Elastic properties of induced pluripotent stem cells.
The recent technique of transducing key transcription factors into unipotent cells (fibroblasts) to generate pluripotent stem cells (induced pluripotent stem cells [iPSCs]) has significantly changed the stem cell field. These cells have great promise for many clinical applications, including that of regenerative medicine. Our findings show that iPSCs can be derived from human adipose-derived stromal cells (hASCs), a notable advancement in the clinical applicability of these cells. To investigate differences between two iPS cell lines (fibroblast-iPSC and hASC-iPSC), and also the gold standard human embryonic stem cell, we looked at cell stiffness as a possible indicator of cell differentiation-potential differences. We used atomic force microscopy as a tool to determine stem cell stiffness, and hence differences in material properties between cells. Human fibroblast and hASC stiffness was also ascertained for comparison. Interestingly, cells exhibited a noticeable difference in stiffness. From least to most stiff, the order of cell stiffness was as follows: hASC-iPSC, human embryonic stem cell, fibroblast-iPSC, fibroblasts, and, lastly, as the stiffest cell, hASC. In comparing hASC-iPSCs to their origin cell, the hASC, the reprogrammed cell is significantly less stiff, indicating that greater differentiation potentials may correlate with a lower cellular modulus. The stiffness differences are not dependent on cell culture density; hence, material differences between cells cannot be attributed solely to cell-cell constraints. The change in mechanical properties of the cells in response to reprogramming offers insight into how the cell interacts with its environment and might lend clues to how to efficiently reprogram cell populations as well as how to maintain their pluripotent state.
Hammerick
Kyle E
KE
Rapid Prototyping Laboratory, Department of Mechanical Engineering, School of Engineering, Stanford University, Stanford, California, USA.
Huang
Zubin
Z
Sun
Ning
N
Lam
Mai T
MT
Prinz
Fritz B
FB
Wu
Joseph C
JC
Commons
George W
GW
Longaker
Michael T
MT
D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D055119:Elastic Modulus / Q000502:physiology; D005347:Fibroblasts / Q000166:cytology* / Q000502:physiology*; D006801:Humans; D018625:Microscopy, Atomic Force / Q000379:methods*; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000502:physiology*
Stem Cell Res. 2009 Mar;2(2):113-24
19383417
FASEB J. 2006 May;20(7):811-27
16675838
Proc Natl Acad Sci U S A. 2009 Sep 15;106(37):15720-5
19805220
Eur J Cell Biol. 2009 May;88(5):273-83
19167776
J Biomech Eng. 2009 Jun;131(6):061011
19449965
Cell. 2007 Nov 30;131(5):861-72
18035408
Pediatr Surg Int. 2010 Jan;26(1):3-10
19727766
Nat Mater. 2010 Jan;9(1):82-8
19838182
J Biomech. 2008;41(9):2055-9
18501365
Stem Cells. 2009 Sep;27(9):2092-102
19544411
Cell. 2006 Aug 25;126(4):677-89
16923388
J Craniomaxillofac Surg. 2004 Dec;32(6):370-3
15555520
Nat Biotechnol. 2004 May;22(5):560-7
15077117