title: Ivermectin impairs sexual behavior in sexually naïve, but not sexually experienced male rats.
abstract: Ivermectin (IVM) is an antiparasitic drug, widely used in domestic animals. In mammals, IVM act as a GABA agonist. This neurotransmitter has an important role in the regulation of sexual behavior. Thus, this study sought to investigate the effects of various medically relevant doses IVM on the sexual behavior of male rats. In particular, we also wished to examine if previous sexual experience modulated responses to IVM. In the first experiment, the sexual behavior of inexperienced male rats was analyzed after they received 0.2, 0.6, 1.0 or 2.0 mg/kg IVM, 15 min prior to behavioral testing. In the second experiment, the effects of four previous sexual experiences on IVM treated rats (1.0 or 2.0 mg/kg, 15 min prior to the 5th session) were assessed. The standard therapeutic dose (0.2 mg/kg) did not impair the sexual behavior of inexperienced male rats. At a more concentrated dose (0.6 mg/kg), which is still within the therapeutic range, the appetitive phase of sexual behavior of inexperienced male rats was impaired. Likewise, 1.0 mg/kg impaired the appetitive phase. Previous sexual experience blocked almost entirely this sexual impairment, suggesting that previous sexual experience exerts a positive effect in attenuating the sexual impairment produced by IVM treatment. Therefore, the standard therapeutic dose of IVM can be used without producing side effects on sexual behavior. Use of more concentrated therapeutic doses is not recommended during reproductive periods, unless the animals have had previous sexual experience.
authors.affiliation: Health Science Institute, Paulista University, São Paulo, Brazil; Department of Pathology, School of Veterinary Medicine, University of São Paulo, São Paulo, Brazil. Electronic address: marthabernardi@gmail.com.
authors.forename: M M
authors.initials: MM
authors.lastname: Bernardi
authors.affiliation: Department of Pathology, School of Veterinary Medicine, University of São Paulo, São Paulo, Brazil.
authors.forename: T B
authors.initials: TB
authors.lastname: Kirsten
authors.affiliation: Department of Pathology, School of Veterinary Medicine, University of São Paulo, São Paulo, Brazil.
authors.forename: H S
authors.initials: HS
authors.lastname: Spinosa
authors.affiliation: Health Science Institute, Paulista University, São Paulo, Brazil.
authors.forename: H
authors.initials: H
authors.lastname: Manzano
mesh_terms: D000818:Animals; D000977:Antiparasitic Agents / Q000009:adverse effects*; D004305:Dose-Response Relationship, Drug; D005260:Female; D018755:GABA Agonists / Q000009:adverse effects*; D007559:Ivermectin / Q000009:adverse effects*; D008297:Male; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012726:Sexual Behavior, Animal / Q000187:drug effects*
chemical_list: D000977:Antiparasitic Agents; D018755:GABA Agonists; D007559:Ivermectin