Characterization of proteins from kernel of different soybean varieties.
BACKGROUND
Total soybean proteins, storage proteins, glycinin (11S) and β-conglycinin (7S) fractions and their respective subunits in seven soybean varieties were analyzed. In this work we also present the correlation between concentration and activity of bioactive proteins, lipoxygenase and proteinase inhibitors.


RESULTS
Glycinin and β-conglycinin comprise about 750 g kg(-1) of the bean storage protein and as such account for both quantity and quality of the kernel protein. The 11S concentration of the varieties studied ranged from 503.4 to 602.9 g kg(-1) and those of 7S varied from 178.2 to 230.6 g kg(-1) of total extractable proteins. The ratio of 11S/7S proteins varied from 2.43 to 3.29 among the varieties. A very strong positive correlation was found between the concentration of Kunitz trypsin inhibitor and activity of total trypsin inhibitor (r = 0.96). However, lipoxygenase concentration did not show a strong correlation with lipoxygenase activity.


CONCLUSIONS
It appears that among the seven ZP soybean genotypes there are genotypes with different amounts of subunits that should be bred in the future for a desired level of protein components.
Zilić
Sladana M
SM
Maize Research Institute, Zemun Polje, Department of Technology, Belgrade-Zemun, Serbia. szilic@mrizp.rs
Barać
Miroljub B
MB
Pesić
Mirjana B
MB
Mladenović Drinić
Snezana D
SD
Ignjatović-Micić
Dragana D
DD
Srebrić
Mirjana B
MB
D005916:Globulins / Q000032:analysis*; D008084:Lipoxygenase / Q000378:metabolism; D010940:Plant Proteins / Q000032:analysis*; D021122:Protein Subunits / Q000032:analysis; D012639:Seeds / Q000737:chemistry*; D030262:Soybean Proteins / Q000737:chemistry*; D013025:Glycine max / Q000737:chemistry* / Q000145:classification / Q000378:metabolism; D013045:Species Specificity; D014361:Trypsin Inhibitors / Q000378:metabolism*
D005916:Globulins; D010940:Plant Proteins; D021122:Protein Subunits; D030262:Soybean Proteins; D014361:Trypsin Inhibitors; D008084:Lipoxygenase