Two Japanese Corynebacterium ulcerans isolates from the same hospital: ribotype, toxigenicity and serum antitoxin titre.
Two toxigenic Corynebacterium ulcerans isolates recovered from pharyngeal swabs of two patients from the same hospital in Japan during 2001-2002 were characterized by PFGE and ribotyping. Toxin production in different culture media was examined and serological analysis of patient sera was performed. The two isolates could not be distinguished by PFGE; however, their ribotypes were distinguishable. One of the isolates could represent a novel ribotype. Analysis of toxin production in different culture media demonstrated that the two isolates produced varying amounts of the diphtheria toxin. Serological analysis showed a greater than sevenfold increase in the serum antitoxin titre during the course of infection in one patient.
Komiya
Takako
T
Department of Bacteriology II, National Institute of Infectious Diseases, Tokyo, Japan.
Seto
Yukiji
Y
Infectious Diseases Control, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Osaka, Japan.
De Zoysa
Aruni
A
Streptococcus and Diphtheria Reference Unit, Respiratory and Systemic Infections Department, Health Protection Agency Centre for Infections, London, UK.
Iwaki
Masaaki
M
Department of Bacteriology II, National Institute of Infectious Diseases, Tokyo, Japan.
Hatanaka
Akio
A
Asahi General Hospital, Chiba, Japan.
Tsunoda
Atsunobu
A
Asahi General Hospital, Chiba, Japan.
Arakawa
Yoshichika
Y
Department of Bacteriology II, National Institute of Infectious Diseases, Tokyo, Japan.
Kozaki
Shunji
S
Infectious Diseases Control, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Osaka, Japan.
Takahashi
Motohide
M
Department of Bacteriology II, National Institute of Infectious Diseases, Tokyo, Japan.
D000992:Antitoxins / Q000097:blood*; D001427:Bacterial Toxins; D003352:Corynebacterium / Q000145:classification* / Q000302:isolation & purification*; D003354:Corynebacterium Infections / Q000453:epidemiology / Q000382:microbiology*; D005260:Female; D005838:Genotype; D006801:Humans; D007564:Japan / Q000453:epidemiology; D008297:Male; D008875:Middle Aged; D021521:Ribotyping
D000992:Antitoxins; D001427:Bacterial Toxins