title: A processed multidomain mycoplasma hyopneumoniae adhesin binds fibronectin, plasminogen, and swine respiratory cilia.
abstract: Porcine enzootic pneumonia is a chronic respiratory disease that affects swine. The etiological agent of the disease, Mycoplasma hyopneumoniae, is a bacterium that adheres to cilia of the swine respiratory tract, resulting in loss of cilia and epithelial cell damage. A M. hyopneumoniae protein P116, encoded by mhp108, was investigated as a potential adhesin. Examination of P116 expression using proteomic analyses observed P116 as a full-length protein and also as fragments, ranging from 17 to 70 kDa in size. A variety of pathogenic bacterial species have been shown to bind the extracellular matrix component fibronectin as an adherence mechanism. M. hyopneumoniae cells were found to bind fibronectin in a dose-dependent and saturable manner. Surface plasmon resonance was used to show that a recombinant C-terminal domain of P116 bound fibronectin at physiologically relevant concentrations (K(D) 24 ± 6 nm). Plasmin(ogen)-binding proteins are also expressed by many bacterial pathogens, facilitating extracellular matrix degradation. M. hyopneumoniae cells were found to also bind plasminogen in a dose-dependent and saturable manner; the C-terminal domain of P116 binds to plasminogen (K(D) 44 ± 5 nm). Plasminogen binding was abolished when the C-terminal lysine of P116 was deleted, implicating this residue as part of the plasminogen binding site. P116 fragments adhere to the PK15 porcine kidney epithelial-like cell line and swine respiratory cilia. Collectively these data suggest that P116 is an important adhesin and virulence factor of M. hyopneumoniae.
authors.affiliation: Schools of Biological Sciences, University of Wollongong, New South Wales 2522, Australia.
authors.forename: Lisa M
authors.initials: LM
authors.lastname: Seymour
authors.forename: Ania T
authors.initials: AT
authors.lastname: Deutscher
authors.forename: Cheryl
authors.initials: C
authors.lastname: Jenkins
authors.forename: Tracey A
authors.initials: TA
authors.lastname: Kuit
authors.forename: Linda
authors.initials: L
authors.lastname: Falconer
authors.forename: F Chris
authors.initials: FC
authors.lastname: Minion
authors.forename: Ben
authors.initials: B
authors.lastname: Crossett
authors.forename: Matthew
authors.initials: M
authors.lastname: Padula
authors.forename: Nicholas E
authors.initials: NE
authors.lastname: Dixon
authors.forename: Steven P
authors.initials: SP
authors.lastname: Djordjevic
authors.forename: Mark J
authors.initials: MJ
authors.lastname: Walker
mesh_terms: D018829:Adhesins, Bacterial / Q000737:chemistry*; D000818:Animals; D002923:Cilia / Q000378:metabolism*; D005353:Fibronectins / Q000737:chemistry*; D008168:Lung / Q000382:microbiology; D008863:Microspheres; D045705:Mycoplasma hyopneumoniae / Q000378:metabolism*; D010957:Plasmids / Q000378:metabolism; D010958:Plasminogen / Q000737:chemistry*; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D040901:Proteomics / Q000379:methods; D013552:Swine
chemical_list: D018829:Adhesins, Bacterial; D005353:Fibronectins; D010958:Plasminogen
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