Increased intramuscular nerve branching and inhibition of programmed cell death of chick embryo motoneurons by immunoglobulins from patients with motoneuron disease.
Massive programmed cell death (PCD) of developing chick embryo motoneurons (MNs) occurs in a well defined temporal and spatial sequence between embryonic day (E) 6 and E10. We have found that, when administered in ovo, either circulating immunoglobulins G (IgGs) or cerebrospinal fluid from patients with MN disease can rescue a significant number of chick embryo MNs from normally occurring PCD. An increase of branching of intramuscular nerves was also observed that may account for the rescuing effects of pathologic IgGs. Proteomic analysis and further analysis by ELISA indicated that these effects may be mediated by the interaction of circulating human immunoglobulins with proteins of the semaphorin family.
Hernández
Sara
S
Universitat de Lleida, Facultat de Medicina, Departament de Medicina Experimental, Lleida, Catalonia, Spain.
Texidó
Laura
L
Calderó
Jordi
J
Ciutat
Dolors
D
Piedrafita
Lídia
L
Casanovas
Anna
A
Blasi
Joan
J
Solsona
Carles
C
Povedano
Mònica
M
Rojas
Ricardo
R
Illa
Isabel
I
Caress
James
J
Prevette
David
D
Oppenheim
Ronald W
RW
Milligan
Carol
C
Esquerda
Josep E
JE
D000704:Analysis of Variance; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002642:Chick Embryo; D002522:Chlorocebus aethiops; D004305:Dose-Response Relationship, Drug; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D005727:Ganglia, Spinal / Q000166:cytology; D020439:Growth Cones / Q000187:drug effects; D006801:Humans; D007136:Immunoglobulins / Q000276:immunology / Q000494:pharmacology*; D066298:In Vitro Techniques; D008297:Male; D016472:Motor Neuron Disease / Q000097:blood / Q000276:immunology*; D009046:Motor Neurons / Q000166:cytology / Q000187:drug effects*; D018482:Muscle, Skeletal / Q000196:embryology / Q000294:innervation*; D009469:Neuromuscular Junction / Q000187:drug effects* / Q000502:physiology; D040901:Proteomics / Q000379:methods; D039961:Semaphorins / Q000378:metabolism; D044967:Serum / Q000737:chemistry / Q000276:immunology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D013223:Statistics as Topic; D018709:Statistics, Nonparametric; D014162:Transfection / Q000379:methods; D014404:Tubulin / Q000378:metabolism
D007136:Immunoglobulins; D039961:Semaphorins; D014404:Tubulin