[Cloning of keratinocyte growth factor 2 gene (KGF2) and its transformation to Brassica napus L].
Recently, more research about the plant bioreactor expressing genes encoding human proteins was reported. In the present study, the cDNA of the human gene keratinocyte growth factor 2 (KGF2) was replaced with plant preferred codons by PCR, and the modified full-length cDNA was cloned into the plant expression vector pCAMBIA-YO containing the oil-body promoter. The fusion construct pCAMBIA-YO-KGF2 was transformed into Brassica napus by Agrobacterium tumefacien-mediated cotyledon transformation method. The transgenic seedlings were identified by PCR, Southern and western blot analysis all showed that KGF2 gene was successfully expressed in in transgenic Brassica napus.
Pan
Guoqing
G
Engineering Research Center of Bioreactor and Pharmaceutical Development, Ministry of Education, Jilin Agricultural University, Changchun 130118, China.
Zhang
Shuang
S
Liu
Xiuming
X
Li
Ying
Y
Zhang
Yaofang
Y
Li
Hongzhi
H
Li
Haiyan
H
Li
Xiaokun
X
D029688:Brassica napus / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D051523:Fibroblast Growth Factor 7 / Q000096:biosynthesis / Q000235:genetics*; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D030821:Plants, Genetically Modified / Q000235:genetics*; D012231:Rhizobium / Q000235:genetics; D014170:Transformation, Genetic
D018076:DNA, Complementary; C496453:FGF7 protein, human; D051523:Fibroblast Growth Factor 7