Association of 5'-CpG island hypermethylation of the FHIT gene with lung cancer in southern-central Chinese population.
The promoter methylation of the FHIT gene has been associated with susceptibility to different cancers, including a role in the early pathogenesis of lung cancer. We investigated the aberrant promoter methylation of FHIT in lung cancer in the Han population of southern-central China. Blood samples from 123 lung cancer patients of different clinical stage and histological grading and 105 healthy control samples were collected. Methylation-specific polymerase chain reaction (MSP ) was performed to analyze the methylation status of FHIT. Aberrant promoter methylation of the FHIT gene was 34.15% (42/123) in cancer patients, but in none of the 105 controls. Significant association was found between the lung cancer cases and controls (OR=2.296; 95% CI=1.95-2.705; p < 0.01). Furthermore, we found that aberrant promoter methylation of the FHIT gene showed a highly significant association with clinical stage I and not with clinical stage IV in lung cancer (p < 0.05). These findings suggest FHIT methylation is associated with a higher susceptibility and has a prognostic significance in early stage lung cancer in the Han population of southern-central China and may represent a marker for progressive disease.
Li
Wen
W
Central South University, ChangSha, Hunan Province, China.
Deng
Jing
J
Jiang
Pei
P
Tang
Jianxin
J
D017766:Acid Anhydride Hydrolases / Q000235:genetics*; D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D044466:Asian People / Q000235:genetics*; D014408:Biomarkers, Tumor / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D016022:Case-Control Studies; D018899:CpG Islands / Q000235:genetics*; D019175:DNA Methylation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D020868:Gene Silencing; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000235:genetics*; D009367:Neoplasm Staging; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic / Q000235:genetics; D018570:Risk Assessment; D000099170:Bis(5'-adenosyl)triphosphatase
D017766:Acid Anhydride Hydrolases; D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins; D000099170:Bis(5'-adenosyl)triphosphatase