title: Localization microscopy reveals expression-dependent parameters of chromatin nanostructure.
abstract: A combined approach of 2D high-resolution localization light microscopy and statistical methods is presented to infer structural features and density fluctuations at the nuclear nanoscale. Hallmarks of nuclear nanostructure are found on the scale below 100 nm for both human fibroblast and HeLa cells. Mechanical measures were extracted as a quantitative tool from the histone density fluctuations inside the cell to obtain structural fluctuations on the scale of several micrometers. Results show that different mechanisms of expression of the same nuclear protein type lead to significantly different patterns on the nanoscale and to pronounced differences in the detected compressibility of chromatin. The observed fluctuations, including the experimental evidence for dynamic looping, are consistent with a recently proposed chromatin model.
authors.affiliation: Institute for Theoretical Physics, Heidelberg University, Heidelberg, Germany. bohn@tphys.uni-heidelberg.de
authors.forename: Manfred
authors.initials: M
authors.lastname: Bohn
authors.forename: Philipp
authors.initials: P
authors.lastname: Diesinger
authors.forename: Rainer
authors.initials: R
authors.lastname: Kaufmann
authors.forename: Yanina
authors.initials: Y
authors.lastname: Weiland
authors.forename: Patrick
authors.initials: P
authors.lastname: Müller
authors.forename: Manuel
authors.initials: M
authors.lastname: Gunkel
authors.forename: Alexa
authors.initials: A
authors.lastname: von Ketteler
authors.forename: Paul
authors.initials: P
authors.lastname: Lemmer
authors.forename: Michael
authors.initials: M
authors.lastname: Hausmann
authors.forename: Dieter W
authors.initials: DW
authors.lastname: Heermann
authors.forename: Christoph
authors.initials: C
authors.lastname: Cremer
mesh_terms: D002467:Cell Nucleus / Q000378:metabolism; D002843:Chromatin / Q000737:chemistry* / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000378:metabolism; D005786:Gene Expression Regulation; D006367:HeLa Cells; D006657:Histones / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008027:Light; D008853:Microscopy / Q000379:methods* / Q000706:statistics & numerical data; D049329:Nanostructures; D021381:Protein Transport
chemical_list: D002843:Chromatin; D005456:Fluorescent Dyes; D006657:Histones
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