Targeting the reversibly oxidized protein tyrosine phosphatase superfamily.
Controlled production of reactive oxygen species leads to reversible oxidation of protein tyrosine phosphatases (PTPs) and has emerged as an important tier of regulation over phosphorylation-dependent signal transduction. We present a modified cysteinyl-labeling assay that detects reversible oxidation of members of each of the different PTP subclasses. Here, we describe the methods for enriching reversibly oxidized PTPs from complex protein extracts, illustrating the procedure in IMR90 fibroblasts.
Boivin
Benoit
B
Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA.
Yang
Ming
M
Tonks
Nicholas K
NK
D000818:Animals; D006801:Humans; D010084:Oxidation-Reduction; D010766:Phosphorylation; D017027:Protein Tyrosine Phosphatases / Q000032:analysis* / Q000737:chemistry* / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000737:chemistry* / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology
D017382:Reactive Oxygen Species; D017027:Protein Tyrosine Phosphatases
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