title: Acquisition of iron by alkaliphilic bacillus species.
abstract: The biochemical and molecular mechanisms used by alkaliphilic bacteria to acquire iron are unknown. We demonstrate that alkaliphilic (pH > 9) Bacillus species are sensitive to artificial iron (Fe³+) chelators and produce iron-chelating molecules. These alkaliphilic siderophores contain catechol and hydroxamate moieties, and their synthesis is stimulated by manganese(II) salts and suppressed by FeCl₃ addition. Purification and mass spectrometric characterization of the siderophore produced by Caldalkalibacillus thermarum failed to identify any matches to previously observed fragmentation spectra of known siderophores, suggesting a novel structure.
authors.affiliation: Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand.
authors.forename: Duncan G G
authors.initials: DG
authors.lastname: McMillan
authors.forename: Imelda
authors.initials: I
authors.lastname: Velasquez
authors.forename: Brook L
authors.initials: BL
authors.lastname: Nunn
authors.forename: David R
authors.initials: DR
authors.lastname: Goodlett
authors.forename: Keith A
authors.initials: KA
authors.lastname: Hunter
authors.forename: Iain
authors.initials: I
authors.lastname: Lamont
authors.forename: Sylvia G
authors.initials: SG
authors.lastname: Sander
authors.forename: Gregory M
authors.initials: GM
authors.lastname: Cook
mesh_terms: D001406:Bacillaceae / Q000378:metabolism*; D002396:Catechols / Q000032:analysis; D002712:Chlorides / Q000378:metabolism; D005290:Ferric Compounds / Q000378:metabolism; D006877:Hydroxamic Acids / Q000032:analysis; D007501:Iron / Q000378:metabolism*; D007502:Iron Chelating Agents / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D008345:Manganese / Q000378:metabolism; D013058:Mass Spectrometry; D017262:Siderophores / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D002396:Catechols; D002712:Chlorides; D005290:Ferric Compounds; D006877:Hydroxamic Acids; D007502:Iron Chelating Agents; D017262:Siderophores; D008345:Manganese; D007501:Iron; C034221:catechol; C024555:ferric chloride
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