High-throughput profiling in the hematopoietic system.
The expression profile of microRNAs significantly varies in physiological and pathological conditions. Increasing evidence from the literature shows that abnormalities of the miRNome (defined as the full spectrum of miRNAs expressed in a genome) occur in almost all human diseases and have important pathogenetic, prognostic, and therapeutic implications. The study of the aberrancies of the miRNome has become possible by developing high-throughput profiling techniques that allow the simultaneous detection of differences in miRNA expression between normal and pathologic tissues or simply tissues at different stages of differentiation. These techniques provide the basis for further investigations focused on the miRNAs, which are most frequently and widely differentially expressed under the different investigated conditions.
Fabbri
Muller
M
Department of Molecular Virology, Immunology and Medical Genetics, Ohio State University, Columbus, OH, USA. muller.fabbri@osumc.edu
Spizzo
Riccardo
R
Calin
George A
GA
D000818:Animals; D020869:Gene Expression Profiling / Q000295:instrumentation / Q000379:methods; D006413:Hematopoietic System / Q000502:physiology*; D059014:High-Throughput Nucleotide Sequencing / Q000295:instrumentation / Q000379:methods; D006801:Humans; D035683:MicroRNAs / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D009369:Neoplasms / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation / Q000379:methods; D025341:Principal Component Analysis
D035683:MicroRNAs