title: Effects of anticoagulants on human plasma soluble corin levels measured by ELISA.
abstract: BACKGROUND
  Recently, soluble corin was detected in human plasma. In patients with heart failure, plasma corin levels were lower than that of normal controls. In this study, we analyzed experimental conditions for measuring plasma or serum corin by an immunoassay.
  
  
  METHODS
  Serum and plasma corin levels were measured by ELISA. Effects of different anticoagulants (EDTA, heparin and sodium citrate) on plasma corin levels were examined.
  
  
  RESULTS
  Corin levels in serum were similar to that in plasma with heparin (950±305 vs. 929±301 pg/ml, n=40, p=0.73), but were significantly higher than those in plasma with sodium citrate (735±237 pg/ml, p<0.01) or EDTA (716±261 pg/ml, p<0.001). Native and recombinant human corin proteins were stable in human plasma with EDTA at 4°C or underwent freezing-and-thawing. In 348 healthy Chinese individuals, plasma corin levels ranged from 216 to 1663 pg/ml. The levels were higher in males than that in females (842±283 vs. 569±192 pg/ml, p<0.001).
  
  
  CONCLUSIONS
  Soluble corin was stable in plasma samples. Plasma soluble corin levels vary depending on anticoagulants used. Samples containing heparin had significantly higher levels of corin than that in samples with EDTA or sodium citrate.
authors.affiliation: Cyrus Tang Hematology Center, Jiangsu Institute of Hematology, First Affiliated Hospital, Soochow University, Suzhou, China. ningzheng_dong@yahoo.com.cn
authors.forename: Ningzheng
authors.initials: N
authors.lastname: Dong
authors.forename: Jing
authors.initials: J
authors.lastname: Dong
authors.forename: Peng
authors.initials: P
authors.lastname: Liu
authors.forename: Luyao
authors.initials: L
authors.lastname: Xu
authors.forename: Sengseng
authors.initials: S
authors.lastname: Shi
authors.forename: Qingyu
authors.initials: Q
authors.lastname: Wu
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000925:Anticoagulants / Q000494:pharmacology*; D016477:Artifacts; D015415:Biomarkers / Q000097:blood; D001774:Blood Chemical Analysis / Q000379:methods* / Q000592:standards; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000592:standards; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011994:Recombinant Proteins / Q000097:blood; D012016:Reference Values; D012697:Serine Endopeptidases / Q000097:blood*; D012995:Solubility; D055815:Young Adult
chemical_list: D000925:Anticoagulants; D015415:Biomarkers; D011994:Recombinant Proteins; C119812:CORIN protein, human; D012697:Serine Endopeptidases
references.citation: Circ Heart Fail. 2010 Mar;3(2):207-11
references.pmid: 20061521
references.citation: Proc Natl Acad Sci U S A. 2000 Jul 18;97(15):8525-9
references.pmid: 10880574
references.citation: Circulation. 2005 Oct 18;112(16):2403-10
references.pmid: 16216958
references.citation: J Am Coll Cardiol. 2007 Dec 18;50(25):2357-68
references.pmid: 18154959
references.citation: J Biol Chem. 1999 May 21;274(21):14926-35
references.pmid: 10329693
references.citation: J Biol Chem. 2004 Aug 13;279(33):34464-71
references.pmid: 15192093
references.citation: J Biol Chem. 2007 Sep 21;282(38):27728-35
references.pmid: 17660514
references.citation: N Engl J Med. 2008 May 15;358(20):2148-59
references.pmid: 18480207
references.citation: Clin Chem. 2008 Mar;54(3):619-21
references.pmid: 18310156
references.citation: J Card Fail. 2010 Aug;16(8):621-7
references.pmid: 20670840
references.citation: J Biol Chem. 1999 Jun 25;274(26):18237-42
references.pmid: 10373425
references.citation: Am J Clin Pathol. 2006 Aug;126(2):174-84
references.pmid: 16891190
references.citation: Circ Heart Fail. 2009 Nov;2(6):541-8
references.pmid: 19919978
references.citation: N Engl J Med. 2002 Jul 18;347(3):161-7
references.pmid: 12124404
references.citation: N Engl J Med. 1998 Jul 30;339(5):321-8
references.pmid: 9682046
references.citation: Front Biosci. 2007 May 01;12:4179-90
references.pmid: 17485366
references.citation: Kidney Int. 2009 Jan;75(2):142-6
references.pmid: 18716601
references.citation: Proc Natl Acad Sci U S A. 2005 Jan 18;102(3):785-90
references.pmid: 15637153
references.citation: Am Heart J. 2006 Nov;152(5):828-34
references.pmid: 17070141
references.citation: J Biol Chem. 2009 Aug 28;284(35):23177-81
references.pmid: 19487698
references.citation: Heart. 2010 Mar;96(6):419-24
references.pmid: 19451138
references.citation: Front Biosci. 2001 Feb 01;6:D192-200
references.pmid: 11171558
references.citation: Am J Pathol. 2008 Dec;173(6):1693-701
references.pmid: 18988802
references.citation: Circ Res. 2008 Aug 29;103(5):502-8
references.pmid: 18669922
references.citation: Hypertension. 2007 Apr;49(4):857-64
references.pmid: 17296875
references.citation: Clin Chim Acta. 2005 Oct;360(1-2):122-7
references.pmid: 16002063
references.citation: Development. 2008 Jan;135(2):217-25
references.pmid: 18057101
references.citation: J Biol Chem. 2003 Dec 26;278(52):52363-70
references.pmid: 14559895
references.citation: Eur J Biochem. 2000 Dec;267(23):6931-7
references.pmid: 11082206
references.citation: Curr Top Dev Biol. 2003;54:167-206
references.pmid: 12696750
references.citation: Clin Chim Acta. 2009 Nov;409(1-2):85-9
references.pmid: 19751717
references.citation: Development. 2007 Sep;134(17):3213-25
references.pmid: 17670789
references.citation: Eur J Heart Fail. 2004 Mar 15;6(3):355-8
references.pmid: 14987588