The L55M polymorphism of paraoxonase-1 is a risk factor for rheumatoid arthritis.
Paraoxonase-1 (PON1) is a high-density lipoprotein-associated enzyme that exhibits antioxidant and antiatherogenic activities. We examined a possible association between T172A (L55M) and T(-107)C polymorphisms and rheumatoid arthritis. These polymorphisms were determined in 88 rheumatoid arthritis patients and 78 healthy subjects, using the tetra-amplification refractory mutation system-PCR method. The prevalence of the PON1 55MM genotype was significantly greater among rheumatoid arthritis patients (17%) when compared to control subjects (5.2%) (odds ratio (OR) = 3.75; 95% confidence interval (CI) = 1.87-11.8, P = 0.025). In addition, the M allele was more frequent in rheumatoid arthritis patients (40%) than in healthy subjects (24.7%) (OR = 1.997; 95%CI = 1.243-3.210, P = 0.005). There were no significant differences in the -107C/T polymorphism in the promoter sequence of PON1 between rheumatoid arthritis and normal subjects (chi(2) = 0.861, P = 0.650). In conclusion, the PON1 55MM genotype is a risk factor for rheumatoid arthritis.
Hashemi
M
M
Department of Clinical Biochemistry, School of Medicine, Zaedan University of Medical Sciences, Zahedan, Iran. mhd.hashemi@gmail.com
Moazeni-Roodi
A K
AK
Fazaeli
A
A
Sandoughi
M
M
Taheri
M
M
Bardestani
G R
GR
Zakeri
Z
Z
Kordi-Tamandani
D M
DM
Ghavami
S
S
D000328:Adult; D001172:Arthritis, Rheumatoid / Q000235:genetics*; D043303:Aryldialkylphosphatase / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*
D043303:Aryldialkylphosphatase