title: [Study on the function of plasmid in pyridine-degrading bacteria].
abstract: In order to identify the characteristics of the plasmids of degrading bacterial strains and the relationship between the plasmids' function and biodegradation, plasmids were isolated from two bacterial strains (Paracoccus sp. BW001 and Shinella zoogloeoides BC026) and pulse-field gel electrophoresis was used to identify the distribution of plasmids and their molecular size. Two large plasmids with 190-245 kb and one small plasmid with 4.5-5.0 kb were found in the BW001, and at least 3 large plasmids over 200 kb were harbored in the BC026. The plasmid curing was conducted by high temperature-SDS method and the results indicated the biodegradation genes might locate in the plasmids of two bacterial strains. After transforming the plasmids of BW001 into E. coli 5alpha by electroporation, the new bacterial strain could tolerate pyridine.
authors.affiliation: College of Environmental Sciences and Engineering,Peking University, Beijing 100871, China. baiyh@pku.edu.cn
authors.forename: Yao-hui
authors.initials: YH
authors.lastname: Bai
authors.forename: Qing-hua
authors.initials: QH
authors.lastname: Sun
authors.forename: Cui
authors.initials: C
authors.lastname: Zhao
authors.forename: Dong-hui
authors.initials: DH
authors.lastname: Wen
authors.forename: Xiao-yan
authors.initials: XY
authors.lastname: Tang
mesh_terms: D001673:Biodegradation, Environmental; D018274:Electroporation; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D010230:Paracoccus / Q000235:genetics* / Q000502:physiology; D010957:Plasmids / Q000235:genetics / Q000502:physiology*; D011725:Pyridines / Q000302:isolation & purification* / Q000378:metabolism; D012230:Rhizobiaceae / Q000235:genetics* / Q000502:physiology; D014865:Waste Disposal, Fluid / Q000379:methods; D014874:Water Pollutants, Chemical / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D011725:Pyridines; D014874:Water Pollutants, Chemical; C023666:pyridine