title: Enzymatic single-step preparation of multifunctional proteins.
abstract: A small glutamine donor substrate was designed for single-step and site-specific protein multifunctionalization catalyzed by microbial transglutaminase.
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Motooka, Fukuoka 819-0395, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Abe
authors.forename: Masahiro
authors.initials: M
authors.lastname: Goto
authors.forename: Noriho
authors.initials: N
authors.lastname: Kamiya
mesh_terms: D001710:Biotin / Q000737:chemistry; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D005973:Glutamine / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D013302:Streptomyces / Q000201:enzymology*; D013379:Substrate Specificity; D011503:Transglutaminases / Q000378:metabolism*
chemical_list: D005456:Fluorescent Dyes; D011506:Proteins; D011994:Recombinant Proteins; D005973:Glutamine; D001710:Biotin; D011503:Transglutaminases; D016650:Fluorescein-5-isothiocyanate