title: Premature centromere division of metaphase chromosomes in peripheral blood lymphocytes of Alzheimer's disease patients: relation to gender and age.
abstract: Chromosomal alterations are a feature of both aging and Alzheimer's disease (AD). This study examined if premature centromere division (PCD), a chromosomal instability indicator increased in AD, is correlated with aging or, instead, represents a de novo chromosomal alteration due to accelerating aging in AD. PCD in peripheral blood lymphocytes was determined in sporadic AD patients and gender and age-matched unaffected controls. Metaphase nuclei were analyzed for chromosomes showing PCD, X chromosomes with PCD (PCD,X), and acrocentric chromosomes showing PCD. AD patients, regardless of age, demonstrated increased PCD on any chromosome and PCD on acrocentric chromosomes in both genders, whereas an increase in frequency of PCD,X was expressed only in women. This cytogenetic analysis suggests that PCD is a feature of AD, rather than an epiphenomenon of chronological aging, and may be useful as a physiological biomarker that can be used for disease diagnosis.
authors.affiliation: Institute of Physiology, Department of Biology, Faculty of Pharmacy, University of Belgrade, Belgrade, Serbia. lada@pharmacy.bg.ac.rs
authors.forename: Lada
authors.initials: L
authors.lastname: Zivković
authors.forename: Biljana
authors.initials: B
authors.lastname: Spremo-Potparević
authors.forename: Bosiljka
authors.initials: B
authors.lastname: Plecas-Solarović
authors.forename: Ninoslav
authors.initials: N
authors.lastname: Djelić
authors.forename: Gordana
authors.initials: G
authors.lastname: Ocić
authors.forename: Predrag
authors.initials: P
authors.lastname: Smiljković
authors.forename: Sandra L
authors.initials: SL
authors.lastname: Siedlak
authors.forename: Mark A
authors.initials: MA
authors.lastname: Smith
authors.forename: Vladan
authors.initials: V
authors.lastname: Bajić
mesh_terms: D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000097:blood* / Q000235:genetics* / Q000473:pathology; D002455:Cell Division; D002503:Centromere / Q000473:pathology*; D043171:Chromosomal Instability / Q000235:genetics*; D041321:Chromosomes, Human, X; D020732:Cytogenetic Analysis; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000473:pathology*; D008297:Male; D008677:Metaphase; D008875:Middle Aged; D012737:Sex Factors; D013997:Time Factors
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