title: Single laser source for multimodal coherent anti-Stokes Raman scattering microscopy.
abstract: Short laser pulse technology has significantly contributed to biomedical research, especially via nonlinear optical microscopy. Coherent anti-Stokes Raman scattering (CARS) microscopy is a label-free, chemical-selective method that is growing in importance as improved methods and light sources develop. Here we discuss different approaches to laser source development for CARS microscopy and highlight the advantages of a multimodal CARS microscope, illustrated by selected applications in biomedical research.
authors.affiliation: Department of Physics, Queen's University, Kingston, Ontario K7L 3N6, Canada. adrian.pegoraro@nrc-cnrc.gc.ca
authors.forename: Adrian F
authors.initials: AF
authors.lastname: Pegoraro
authors.forename: Aaron D
authors.initials: AD
authors.lastname: Slepkov
authors.forename: Andrew
authors.initials: A
authors.lastname: Ridsdale
authors.forename: John Paul
authors.initials: JP
authors.lastname: Pezacki
authors.forename: Albert
authors.initials: A
authors.lastname: Stolow
mesh_terms: D000818:Animals; D002460:Cell Line; D002477:Cells / Q000648:ultrastructure*; D006801:Humans; D007089:Image Enhancement; D007834:Lasers; D051379:Mice; D008853:Microscopy / Q000295:instrumentation / Q000379:methods*; D013059:Spectrum Analysis, Raman / Q000295:instrumentation / Q000379:methods*