Image-based calculation of perfusion and oxyhemoglobin saturation in skeletal muscle during submaximal isometric contractions.
The relative oxygen saturation of hemoglobin and the rate of perfusion are important physiological quantities, particularly in organs such as skeletal muscle, in which oxygen delivery and use are tightly coupled. The purpose of this study was to demonstrate the image-based calculation of the relative oxygen saturation of hemoglobin and quantification of perfusion in skeletal muscle during isometric contractions. This was accomplished by establishing an empirical relationship between the rate of radiofrequency-reversible dephasing and near-infrared spectroscopy-observed oxyhemoglobin saturation (relative oxygen saturation of hemoglobin) under conditions of arterial occlusion and constant blood volume. A calibration curve was generated and used to calculate the relative oxygen saturation of hemoglobin from radiofrequency-reversible dephasing changes measured during contraction. Twelve young healthy subjects underwent 300 s of arterial occlusion and performed isometric contractions of the dorsiflexors at 30% of maximal contraction for 120 s. Muscle perfusion was quantified during contraction by arterial spin labeling and measures of muscle T(1). Comparisons between the relative oxygen saturation of hemoglobin values predicted from radiofrequency-reversible dephasing and that measured by near-infrared spectroscopy revealed no differences between methods (P = 0.760). Muscle perfusion reached a value of 34.7 mL 100 g(-1) min(-1) during contraction. These measurements hold future promise in measuring muscle oxygen consumption in healthy and diseased skeletal muscle.
Elder
Christopher P
CP
Institute of Imaging Science, Vanderbilt University, Nashville, Tennessee 37232-2310, USA.
Cook
Ryan N
RN
Chance
Marti A
MA
Copenhaver
Elizabeth A
EA
Damon
Bruce M
BM
D000328:Adult; D000465:Algorithms; D001783:Blood Flow Velocity / Q000502:physiology; D003097:Collateral Circulation / Q000502:physiology*; D005260:Female; D006801:Humans; D007089:Image Enhancement / Q000379:methods; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D007537:Isometric Contraction / Q000502:physiology*; D018810:Magnetic Resonance Angiography / Q000379:methods*; D008297:Male; D018482:Muscle, Skeletal / Q000033:anatomy & histology / Q000098:blood supply / Q000502:physiology*; D010108:Oxyhemoglobins / Q000032:analysis*; D010807:Physical Endurance / Q000502:physiology; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D010108:Oxyhemoglobins
NMR Biomed. 2001 May;14(3):199-203
11357185
J Appl Physiol (1985). 1999 Dec;87(6):2375-80
10601192
NMR Biomed. 1994 Mar;7(1-2):35-44
8068524
Brain Res. 1997 Jan 9;744(2):207-15
9027380
Med Image Anal. 1996 Mar;1(1):35-51
9873920
Magn Reson Med. 1997 Mar;37(3):425-35
9055234
Magn Reson Med. 2004 Sep;52(3):679-82
15334591
NMR Biomed. 2006 Feb;19(1):125-32
16404727
Magn Reson Med. 1998 Oct;40(4):551-8
9771572
Proc Natl Acad Sci U S A. 1998 Feb 17;95(4):1834-9
9465103
NMR Biomed. 2006 Nov;19(7):954-67
17075963
Med Sci Sports Exerc. 1998 Sep;30(9):1374-80
9741606
J Cereb Blood Flow Metab. 2000 Aug;20(8):1225-36
10950383
Magn Reson Med. 2009 Mar;61(3):560-9
19097244
Magn Reson Med. 1998 Dec;40(6):857-64
9840830
J Cereb Blood Flow Metab. 1999 Aug;19(8):853-62
10458592
J Magn Reson Imaging. 1998 Sep-Oct;8(5):1106-13
9786149
Biochim Biophys Acta. 1982 Feb 2;714(2):265-70
6275909
Magn Reson Med. 1994 Jun;31(6):678-81
8057821
J Appl Physiol (1985). 2004 Dec;97(6):2385-94
15298991
NMR Biomed. 2004 Oct;17(6):392-8
15468084
NMR Biomed. 1995 Feb;8(1):41-7
7547184
Med Sci Sports Exerc. 2007 Feb;39(2):257-67
17277589
J Appl Physiol Respir Environ Exerc Physiol. 1984 Feb;56(2):287-95
6706739
Magn Reson Imaging. 2006 Sep;24(7):941-51
16916711
J Appl Physiol (1985). 2002 Jan;92(1):129-34
11744651
Magn Reson Med. 1998 Mar;39(3):474-81
9498604
Magn Reson Med. 2009 Jul;62(1):141-8
19353674
Magn Reson Med. 1993 Dec;30(6):715-23
8139453
Magn Reson Med. 1998 Sep;40(3):432-42
9727947
Magn Reson Med. 2003 Jan;49(1):47-60
12509819
Neurosci Lett. 2000 Dec 22;296(2-3):133-6
11108999
Magn Reson Med. 2002 Jan;47(1):14-23
11754438
Magn Reson Med. 2007 Aug;58(2):335-45
17654591
Am Heart J. 1994 Mar;127(3):674-82
8122618
J Appl Physiol (1985). 2005 Jan;98(1):264-73
15333610
Magn Reson Med. 1992 Oct;27(2):391-7
1461120
Magn Reson Med. 1991 Nov;22(1):159-66
1798390
Magn Reson Med. 1997 Jun;37(6):953-6
9178248