Identification of bovine papilloma virus 10 in teat warts of cattle by DNase-SISPA.
Papilloma viruses are detected and identified by PCR with consensus primers designed from human papilloma virus sequences. These and other primers could not detect papilloma virus in bovine teat wart samples despite repeated attempts. DNase-SISPA, a metagenomic method for identifying viruses, could identify bovine papilloma virus type 10 in bovine teat warts. The sequence comparison between consensus primers and bovine papilloma virus type 10 sequences revealed many differences between consensus primers and BPV-10 sequences. We suggest, DNase-SISPA may be used as an alternate method for papilloma virus diagnosis, in cases where PCR fails to identify papilloma viruses.
Rai
Gaurava K
GK
Biochemistry Division, Indian Veterinary Research Institute, Izatnagar 243122, Bareilly, India.
Saxena
Meeta
M
Singh
Vidya
V
Somvanshi
Ramesh
R
Sharma
Bhaskar
B
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002418:Cattle Diseases / Q000821:virology*; D004279:DNA, Viral / Q000235:genetics; D056186:Metagenomics; D027383:Papillomaviridae / Q000235:genetics*; D030361:Papillomavirus Infections / Q000662:veterinary* / Q000821:virology; D016133:Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D014860:Warts / Q000662:veterinary* / Q000821:virology
D004279:DNA, Viral