[Ingredients of Shuanghuanglian injection powder permeation through placental barrier of rat in pregnancy].
OBJECTIVE
Use HPLC to study the permeation of ingredients of Shuanghuanglian injection powder (SHL) through placental barriers of rats at different stages of pregnancy.


METHODS
The pregnant rats were administered SHL for 5 d through caudalis vena at different stages of pregnancy. Plasma and embryonic tissues were obtained 12 h after the final administration of SHL. The componds in biological specimen were identified by HPLC.


RESULTS
Baicalin, luteolin and wogonoside were the main compounds in plasma. Wogonoside retained in first trimester embryonic tissues, and baicalin retained in the embryonic tissues of different pregnant stages.


CONCLUSIONS
Baicalin is the main compound of SHL through placental barriers of rats. Embryotoxicity of baicalin should be considered as the key point to evaluate the safety of SHL.
Song
Dianrong
D
Gynecology of Second Affiliated Hospital Tianjin University of Traditional Chinese Medicine, Tianjin 300150, China. Songdr58@126.com
Guo
Jie
J
Wang
Yuefei
Y
Pan
Guixiang
G
Li
Peilin
P
Zhang
Wei
W
Song
Hongyun
H
D000818:Animals; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000032:analysis / Q000378:metabolism*; D005260:Female; D006801:Humans; D007267:Injections; D008297:Male; D023421:Models, Animal; D010539:Permeability; D010920:Placenta / Q000187:drug effects / Q000378:metabolism*; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar
D004365:Drugs, Chinese Herbal