title: Bone morphogenetic protein-7 promotes chondrogenesis in human amniotic epithelial cells.
abstract: Bone morphogenetic proteins (BMPs) play important roles at multiple stages of chondrogenesis. This study was undertaken to investigate the potential role of bone morphogenetic protein-7 (BMP-7) in the differentiation of chondrocytes using tissue engineering techniques. The impact of BMP-7 on human amniotic epithelial cells (hAECs) was tested. The hAECs were treated either with recombinant human BMP-7 cDNA or with transforming growth factor beta 1 (TGF-β1) as a positive control for three weeks in vitro. Cartilaginous differentiation and proliferation were assayed by quantitative RT-PCR, histology, and in situ hybridization. Our results were such that hAECs treated with either BMP-7 or TGF-β1 expressed cartilage markers (aggrecan, Sox9, CEP-68, and type II and X collagens) within three weeks. Compared with a control vector, BMP-7 induced a decrease in type I collagen expression, while the transcription of the cartilage-specific type II collagen remained stable. In induction experiments, BMP-7 transgenic hAECs exhibited the largest amount of matrix synthesis. In conclusion, these data indicate that BMP-7 plays an important role in inducing the production of cartilage by hAECs in vitro. Cartilage differentiation and matrix maturation can be promoted by BMPs in a cartilage engineering paradigm. These properties make BMPs promising tools in the engineering of cartilaginous joint bio-prostheses and as candidate biological agents or genes for cartilage stabilisation.
authors.affiliation: Department of Orthopaedic Surgery, Tongji Hospital of Tongji University, Shanghai 200065, China.
authors.forename: Junjie
authors.initials: J
authors.lastname: Zhou
authors.forename: Guangrong
authors.initials: G
authors.lastname: Yu
authors.forename: Chengfu
authors.initials: C
authors.lastname: Cao
authors.forename: Jinhui
authors.initials: J
authors.lastname: Pang
authors.forename: Xianqi
authors.initials: X
authors.lastname: Chen
mesh_terms: D000650:Amnion / Q000166:cytology*; D000954:Antigens, Surface / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D055419:Bone Morphogenetic Protein 7 / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D019902:Chondrocytes / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D020219:Chondrogenesis / Q000187:drug effects* / Q000502:physiology; D024043:Collagen Type II / Q000235:genetics / Q000378:metabolism; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D051457:Hyaline Cartilage / Q000187:drug effects* / Q000254:growth & development; D011994:Recombinant Proteins; D055758:SOX9 Transcription Factor / Q000235:genetics / Q000378:metabolism; D023822:Tissue Engineering; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology
chemical_list: D000954:Antigens, Surface; D015415:Biomarkers; D055419:Bone Morphogenetic Protein 7; D024043:Collagen Type II; D011994:Recombinant Proteins; D055758:SOX9 Transcription Factor; C527932:SOX9 protein, human; D053773:Transforming Growth Factor beta1
references.citation: Osteoarthritis Cartilage. 2007 May;15(5):493-505
references.pmid: 17140817
references.citation: Tissue Eng. 2007 Sep;13(9):2207-18
references.pmid: 17678413
references.citation: Dev Cell. 2002 Sep;3(3):439-49
references.pmid: 12361605
references.citation: Dev Growth Differ. 2000 Jun;42(3):229-36
references.pmid: 10910129
references.citation: Stem Cells. 2007 Jul;25(7):1610-7
references.pmid: 17395773
references.citation: Stem Cells. 2004;22(7):1338-45
references.pmid: 15579651
references.citation: Stem Cells. 2007 Feb;25(2):319-31
references.pmid: 17053211
references.citation: Growth Factors. 2000;17(3):177-92
references.pmid: 10705576
references.citation: J Clin Invest. 2008 Sep;118(9):3098-108
references.pmid: 18677406
references.citation: J Bone Joint Surg Am. 1996 May;78(5):721-33
references.pmid: 8642029
references.citation: Biol Reprod. 2007 Sep;77(3):577-88
references.pmid: 17494917
references.citation: J Orthop Sci. 2007 Jul;12(4):398-404
references.pmid: 17657563
references.citation: J Biol Chem. 1994 Jan 7;269(1):765-70
references.pmid: 8276880
references.citation: Int Orthop. 2002;26(3):131-6
references.pmid: 12073102
references.citation: Int Orthop. 2007 Dec;31(6):719-20
references.pmid: 17704918
references.citation: Growth Factors. 2001;19(2):101-13
references.pmid: 11769970
references.citation: J Cell Physiol. 2007 Aug;212(2):281-4
references.pmid: 17520697
references.citation: Dev Biol. 2000 Mar 15;219(2):237-49
references.pmid: 10694419
references.citation: Proc Natl Acad Sci U S A. 1999 May 11;96(10):5668-73
references.pmid: 10318942
references.citation: Int Orthop. 2007 Dec;31(6):773-81
references.pmid: 17687553
references.citation: Stem Cells. 2007 Mar;25(3):612-20
references.pmid: 17095707
references.citation: J Orthop Res. 2007 Feb;25(2):152-63
references.pmid: 17072841
references.citation: J Orthop Res. 1996 Nov;14(6):937-45
references.pmid: 8982137
references.citation: J Periodontal Res. 2007 Apr;42(2):131-7
references.pmid: 17305871
references.citation: Shock. 2008 May;29(5):603-11
references.pmid: 18414234