title: Desulfovibrio legallis sp. nov.: a moderately halophilic, sulfate-reducing bacterium isolated from a wastewater digestor in Tunisia.
abstract: A new moderately halophilic sulfate-reducing bacterium (strain H₁(T) ) was enriched and isolated from a wastewater digestor in Tunisia. Cells were curved, motile rods (2-3 x 0.5 μm). Strain H₁(T) grew at temperatures between 22 and 43°C (optimum 35°C), and at pH between 5.0 and 9.2 (optimum 7.3-7.5). Strain H₁(T) required salt for growth (1-45 g of NaCl/l), with an optimum at 20-30 g/l. Sulfate, sulfite, thiosulfate, and elemental sulfur were used as terminal electron acceptors but not nitrate and nitrite. Strain H₁(T) utilized lactate, pyruvate, succinate, fumarate, ethanol, and hydrogen (in the presence of acetate and CO₂) as electron donors in the presence of sulfate as electron acceptor. The main end-products from lactate oxidation were acetate with H₂ and CO₂. The G + C content of the genomic DNA was 55%. The predominant fatty acids of strain H₁(T) were C(15:0) iso (38.8%), C(16:0) (19%), and C(14:0) iso 3OH (12.2%), and menaquinone MK-6 was the major respiratory quinone. Phylogenetic analysis of the small-subunit (SSU) ribosomal RNA (rRNA) gene sequence indicated that strain H₁(T) was affiliated to the genus Desulfovibrio. On the basis of SSU rRNA gene sequence comparisons and physiological characteristics, strain H₁(T) is proposed to be assigned to a novel species of sulfate reducers of the genus Desulfovibrio, Desulfovibrio legallis sp. nov. (= DSM 19129(T) = CCUG 54389(T)).
authors.affiliation: INSAT, Laboratoire d'Ecologie et de Technologie Microbienne, Centre Urbain Nord, BP 676, 1080, Tunis Cedex, Tunisia.
authors.forename: Olfa Ben Dhia
authors.initials: OB
authors.lastname: Thabet
authors.forename: Terres
authors.initials: T
authors.lastname: Wafa
authors.forename: Khelifi
authors.initials: K
authors.lastname: Eltaief
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Cayol
authors.forename: Moktar
authors.initials: M
authors.lastname: Hamdi
authors.forename: Guy
authors.initials: G
authors.lastname: Fauque
authors.forename: Marie-Laure
authors.initials: ML
authors.lastname: Fardeau
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002264:Carboxylic Acids / Q000378:metabolism; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D003901:Desulfovibrio / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D005227:Fatty Acids / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology*; D012965:Sodium Chloride / Q000378:metabolism*; D013431:Sulfates / Q000378:metabolism*; D013447:Sulfites / Q000378:metabolism; D013455:Sulfur / Q000378:metabolism; D013696:Temperature; D014416:Tunisia
chemical_list: D002264:Carboxylic Acids; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D013431:Sulfates; D013447:Sulfites; D012965:Sodium Chloride; D013455:Sulfur
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