title: Oxidative stress parameters in paediatric non-alcoholic fatty liver disease.
abstract: We have investigated the presence and the possible clinical implications of oxidative stress in children with non-alcoholic fatty liver disease (NAFLD). The present study was an observational study of oxidative stress parameters in the progression of paediatric NAFLD. We observed the role of oxidative stress in children diagnosed with NAFLD by evaluating: serum protein carbonyls, hepatic expression of 8-hydroxy-2-deoxyguanosine (8-OHG), and circulating antibody against malondialdehyde adducted human serum albumin (MDA-HSA). Forty consecutive children with biopsy-proven NAFLD (27 male; 13 female) referred to Bambino Gesù Children's Hospital, Rome, Italy, from January 2007 to April 2008 were included in the study. Serum variations of protein carbonyls, 8-OHG, and circulating antibody against MDA-HSA were evaluated. Elevated protein carbonyls were evident in 33 subjects (83%) irrespective of obesity and insulin resistance. Moreover, liver biopsies of NAFLD patients positive for circulating protein carbonyls also showed a significant increase in the nuclear staining for 8-OHG (p=0.006; 95% CI 3.1-17.7). Anti-MDA-HSA IgG above control threshold was detected in 25 (63%) children. Although protein carbonyl levels were unrelated with disease severity, patients with elevated anti-MDA-HSA IgG had scores for lobular inflammation significantly higher (p=0.019) than subjects with antibodies within the control range, while steatosis, hepatocyte ballooning and fibrosis were similar. High anti-MDA-HSA reactivity was also associated with a 13-fold increased risk (OR=12.9; 95= CI 1.5-113.8; p=0.013) of a NAFLD activity score (NAS) >or=5. These results demonstrate that oxidative stress has an high prevalence in children with NAFLD and is associated with an increased severity of steatohepatitis.
authors.affiliation: Department of Hepatogastroenterology, Bambino Gesu Children's Hospital, Rome, Italy. nobili66@yahoo.it
authors.forename: Valerio
authors.initials: V
authors.lastname: Nobili
authors.forename: Maurizio
authors.initials: M
authors.lastname: Parola
authors.forename: Anna
authors.initials: A
authors.lastname: Alisi
authors.forename: Fabio
authors.initials: F
authors.lastname: Marra
authors.forename: Fiorella
authors.initials: F
authors.lastname: Piemonte
authors.forename: Christina
authors.initials: C
authors.lastname: Mombello
authors.forename: Salvatore
authors.initials: S
authors.lastname: Sutti
authors.forename: Davide
authors.initials: D
authors.lastname: Povero
authors.forename: Virginia
authors.initials: V
authors.lastname: Maina
authors.forename: Erica
authors.initials: E
authors.lastname: Novo
authors.forename: Emanuele
authors.initials: E
authors.lastname: Albano
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D001798:Blood Proteins / Q000032:analysis / Q000378:metabolism; D002648:Child; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000032:analysis / Q000378:metabolism; D005234:Fatty Liver / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D015227:Lipid Peroxidation; D008099:Liver / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008315:Malondialdehyde / Q000276:immunology; D018384:Oxidative Stress; D012709:Serum Albumin / Q000276:immunology
chemical_list: D001798:Blood Proteins; D007074:Immunoglobulin G; D012709:Serum Albumin; D008315:Malondialdehyde; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine