title: Protection effect of taurine on nitrosative stress in the mice brain with chronic exposure to arsenic.
abstract: BACKGROUND
  Arsenic exposure induces overproduction of reactive nitrogen species (RNS) in brain tissue and results in nucleic acid damage to the nerve cells. The 8-nitroguanine is one of the major products formed by the reaction of guanine, and ONOO-, and has been used as a popular biomarker of nucleic acid damage due to RNS attacking. In the present study, we examined whether the administration of taurine can protect against nucleic acid damage of brain neurons by arsenic-induced RNS.
  
  
  METHODS
  Sixty mice (30 male and 30 female) weighing 19.5 +/- 1.5 g were divided into 3 groups: (1) control group, (2) experimental group that received arsenic (As2O3), and (3) antagonistic group that received taurine with arsenic. Arsenic was administered for 60 days. 8-Nitroguanine expressions in brain neurons of mice were examined by the immunohistochemical method. Histopathological changes in brain tissues of mice were observed under light microscope and the immunohistochemistry method was used to investigate 8-nitroguanine expressions in cerebrum and cerebellum of mice.
  
  
  RESULTS
  In the control group, no abnormal histopathological changes were observed in brain tissue of the mice. In brain tissue of the mice exposed to arsenic, histopathological results showed swells, evident vacuolar degeneration in cytoplasm, karyorrhexis and karyolysis. Relatively light pathological changes were observed in brain of the mice co-administered arsenic and taurine. Little or no expression of 8-nitroguanine in brain tissue was observed in controls. However, intensive expression of 8-nitroguanine was found in brain tissue of mice exposed to arsenic and it was mainly distributed in nucleus neighbouring the nuclear membrane, but a little in cytoplasm. A weak expression of 8-nitroguanine was observed in brain cells of mice co-administered arsenic and taurine.
  
  
  CONCLUSIONS
  The brain neurons may be the major target cells of arsenic neurotoxicity. Co-administration of arsenic and taurine can alleviate DNA damage of brain neurons caused by arsenic through the RNS signal pathway.
authors.affiliation: Faculty of Health Science and Institute of Traditional Chinese Medicine, Suzuka University of Medical Science, Suzuka, Mie 510-0293, Japan. maning@suzuka-u.ac.jp
authors.forename: Ning
authors.initials: N
authors.lastname: Ma
authors.forename: Mikio
authors.initials: M
authors.lastname: Sasoh
authors.forename: Shosuke
authors.initials: S
authors.lastname: Kawanishi
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Sugiura
authors.forename: Fengyuan
authors.initials: F
authors.lastname: Piao
mesh_terms: D000818:Animals; D000077237:Arsenic Trioxide; D001152:Arsenicals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D005260:Female; D006147:Guanine / Q000031:analogs & derivatives / Q000378:metabolism; D006801:Humans; D008297:Male; D051379:Mice; D018696:Neuroprotective Agents / Q000494:pharmacology*; D010087:Oxides / Q000633:toxicity*; D026361:Reactive Nitrogen Species / Q000378:metabolism*; D013654:Taurine / Q000494:pharmacology*
chemical_list: C095838:8-nitroguanine; D001152:Arsenicals; D018696:Neuroprotective Agents; D010087:Oxides; D026361:Reactive Nitrogen Species; D013654:Taurine; D006147:Guanine; D000077237:Arsenic Trioxide
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