[Content detection of bacterial endotoxin in two kinds of injection by gelatin technique].
OBJECTIVE
To detect content of bacterial endotoxin in Yuxingcao and Qingkailing injections by specific and nonspecific tachypleus amebocyte lysate technique for in order to investigate the feasibility of specific tachypleus amebocyte lysate technique for detecting bacterial endotoxin in traditional Chinese drug injections.


METHODS
Different batches of Yuxingcao and Qingkailing injections were detected by specific and nonspecific tachypleus amebocyte lysate kits.


RESULTS
Yuxingcao injection could be detected by specific and nonspecific tachypleus amebocyte lysate technique, Whereas Qingkailing injection could be detected only by specific tachypleus amebocyte lysate.


CONCLUSIONS
Using specific tachypleus amebocyte lysate as a substitute for nonspecific tachypleus amebocyte lysate is an effective method for detecting content of bacterial endotoxin in Qingkailing injection.
Gao
Shuangrong
S
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Liu
Ting
T
Guo
Jing
J
Yi
Yan
Y
Hao
Ran
R
Li
Chunying
C
Cao
Chunyu
C
Zhao
Yong
Y
Hui
Lianqiang
L
Liang
Aihua
A
D000818:Animals; D004340:Drug Contamination; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D004731:Endotoxins / Q000032:analysis*; D006737:Horseshoe Crabs; D008033:Limulus Test / Q000379:methods*
D004365:Drugs, Chinese Herbal; D004731:Endotoxins