1,3-Propandiol production by engineered Hansenula polymorpha expressing dha genes from Klebsiella pneumoniae.
Currently, 1,3-propanediol (1,3-PD) is an important chemical widely used in polymer production, but its availability is being restricted owing to its expensive chemical synthesis. A methylotrophic yeast Hansenula polymorpha was engineered by expression of dhaB1, dhaB2, dhaB3, dhaB(RA1) and dhaB(RA2) encoding glycerol dehydratase complex and dhaT encoding 1,3-PD oxidoreductase from Klebsiella pneumoniae under direction of promoter of glyceraldehyde-3 phosphate dehydrogenase (GAPDH). The engineered recombinant yeast strain can produce 1,3-PD from glucose (2.4 g L(-1)) as well as glycerol (0.8 g L(-1)), which might lead to a safe and cost-effective method for industrial production of 1,3-PD from various biomass resources.
Hong
Won-Kyung
WK
Microbe-based Fusion Technology Research Center, Jeonbuk Branch Institute, KRIBB, Jeongeup, Jeonbuk, South Korea.
Kim
Chul-Ho
CH
Heo
Sun-Yeon
SY
Luo
Lian Hua
LH
Oh
Baek-Rock
BR
Rairakhwada
Dina
D
Seo
Jeong-Woo
JW
D018533:Biomass; D005656:Fungal Proteins / Q000096:biosynthesis* / Q000235:genetics; D015870:Gene Expression; D005800:Genes, Fungal; D005818:Genetic Engineering; D007711:Klebsiella pneumoniae / Q000201:enzymology* / Q000235:genetics; D010843:Pichia / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development*; D011409:Propylene Glycols / Q000378:metabolism*; D013401:Sugar Alcohol Dehydrogenases / Q000096:biosynthesis* / Q000235:genetics
D005656:Fungal Proteins; D011409:Propylene Glycols; C041787:1,3-propanediol; D013401:Sugar Alcohol Dehydrogenases; C020672:glycerol dehydrogenase