Differential effects of taurine treatment and taurine deficiency on the outcome of renal ischemia reperfusion injury.
Taurine possesses membrane stabilization, osmoregulatory and antioxidant properties, aspects of relevance to ischemic injury. We tested the hypothesis that body taurine status is a determinant of renal ischemic injury. Accordingly, renal function and structure were examined in control (C), taurine-treated (TT) and taurine deficient (TD) rats that were subjected to bilateral renal ischemia (60 min) followed by reperfusion (IR); sham operated rats served as controls. Baseline urine osmolality was greater in the TD group than in the control and the TT groups, an effect associated with increased renal aquaporin 2 level. The IR insult reduced urine osmolality (i.e., day-1 post insult); the TD/IR group displayed a more marked recovery in urine osmolality by day-6 post insult than the other two groups. Fluid and sodium excretions were lower in the TD/IR group, suggesting propensity to retention. Histopathological examination revealed the presence of tubular necrotic foci in the C/IR group than sham controls. While renal architecture of the TD/IR group showed features resembling sham controls, the TT/IR group showed dilated tubules, which lacked immunostaining for aquaporin 2, but not 1, suggestive of proximal tubule origin. Finally, assessment of cell proliferation and apoptosis revealed lower proliferation but higher apoptotic foci in the TT/IR group than other IR groups. Collectively, the results indicate that body taurine status is a major determinant of renal IR injury.
Mozaffari
Mahmood S
MS
Department of Oral Biology, Medical College of Georgia School of Dentistry, Augusta, Georgia 30912, USA. Mmozaffa@mail.mcg.edu
Abdelsayed
Rafik
R
Patel
Champa
C
Wimborne
Hereward
H
Liu
Jun Yao
JY
Schaffer
Stephen W
SW
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D051399:Aquaporin 2 / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D007668:Kidney / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D007674:Kidney Diseases / Q000188:drug therapy* / Q000503:physiopathology; D007677:Kidney Function Tests; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000503:physiopathology; D013654:Taurine / Q000172:deficiency* / Q000627:therapeutic use*; D016896:Treatment Outcome
D051399:Aquaporin 2; D013654:Taurine
Kidney Int. 2002 Jun;61(6):2218-27
12028463
J Cardiovasc Pharmacol. 2001 Jun;37(6):742-50
11392471
Crit Care Med. 2003 Aug;31(8 Suppl):S572-81
12907887
Am J Transplant. 2006 Apr;6(4):652-8
16539620
Postgrad Med J. 2006 Feb;82(964):106-16
16461473
J Surg Res. 2008 May 1;146(1):127-34
18061615
Am J Physiol. 1992 Jan;262(1 Pt 2):F117-23
1733287
J Immunol. 2006 Jun 1;176(11):7015-20
16709863
Nephrol Ther. 2005 Dec;1(6):325-34
16895703
Am J Physiol. 1997 Oct;273(4):H1956-61
9362266
Amino Acids. 2007;32(3):405-11
17006602
J Clin Invest. 2004 Jul;114(1):5-14
15232604
Kidney Int. 2004 Aug;66(2):683-95
15253723
J Am Soc Nephrol. 2003 Aug;14(8):2199-210
12874476
Pflugers Arch. 2000 May;440(1):93-9
10864002
J Membr Biol. 2005 Jun;205(3):147-57
16362503
Am J Hypertens. 2003 Aug;16(8):673-80
12878374
Am J Physiol Renal Physiol. 2004 Apr;286(4):F760-6
14644754
Mol Cell Biochem. 2005 Jan;269(1-2):59-67
15786717
Adv Exp Med Biol. 2003;526:307-21
12908615
Am J Physiol Renal Physiol. 2000 Jun;278(6):F925-39
10836980
J Dent Res. 2004 Nov;83(11):823-31
15505230
Physiol Rev. 2007 Oct;87(4):1441-74
17928589
Curr Opin Pediatr. 2005 Apr;17(2):193-9
15800411
J Intern Med. 2007 Jan;261(1):53-64
17222168
J Am Soc Nephrol. 2005 Jul;16(7):2034-43
15901765
Biochimie. 2009 Feb;91(2):261-70
18957317
J Mol Recognit. 2004 Sep-Oct;17(5):473-80
15362107
Nutr Metab (Lond). 2009 Dec 10;6:51
20003253
Am J Physiol Renal Physiol. 2007 Jul;293(1):F269-78
17507599
Curr Opin Clin Nutr Metab Care. 2006 Nov;9(6):728-33
17053427
Am J Physiol. 1995 Sep;269(3 Pt 2):F429-38
7573492
Neurochem Res. 2004 Jan;29(1):117-26
14992270
Kidney Int. 2002 May;61(5):1750-9
11967024
Adv Exp Med Biol. 1994;359:149-57
7887256
Am J Physiol Renal Physiol. 2001 Nov;281(5):F887-99
11592947
Pediatr Res. 2006 Apr;59(4 Pt 2):74R-8R
16549552
Biochem Biophys Res Commun. 2004 Apr 2;316(2):484-9
15020243
J Clin Invest. 2005 Jul;115(7):1705-8
16007248
Am J Physiol Cell Physiol. 2004 Oct;287(4):C949-53
15253891
Kidney Int. 2006 Jul;70(2):329-37
16760912
Acta Physiol (Oxf). 2006 May-Jun;187(1-2):61-73
16734743
Am J Transplant. 2005 Jun;5(6):1276-85
15888031