title: Blastocysts from patients with polycystic ovaries exhibit altered transcriptome and secretome.
abstract: Polycystic ovaries (PCO) is a common phenotype of women presenting for infertility treatment. This study investigated whether blastocysts derived from women with PCO have an altered molecular signature which could be a causative factor contributing to reproductive failure. Morphologically similar blastocysts derived from women with PCO and donor oocyte cycles were analysed for transcription and protein secretion. Unsupervised hierarchical clustering demonstrated that the transcriptome profiles of blastocysts derived from PCO patients and control blastocysts were markedly different with complete branch separation. Statistical analysis revealed 829 genes with significantly different expression: 784 decreased (94.6%) and 45 increased (5.4%) in blastocysts derived from women with PCO compared with controls (P<0.05). Functional annotation of these genes revealed predominant gene ontology biological processes including protein metabolism (30%), transcription (22%), signal transduction (15%), biosynthesis (15%) and cell cycle (14%). Proteomic profiling identified 12 biomarkers that displayed significant decrease in expression in blastocysts derived from women with PCO compared with controls (P<0.05). These data indicate molecular alterations in human blastocysts derived from PCO patients, potentially demonstrating for the first time a link between patient aetiology/phenotype and subsequent embryo development, which in part may explain the observed reduction in reproductive capacity.
authors.affiliation: Colorado Center for Reproductive Medicine, Lone Tree, CO 80124, USA. mkatz-jaffe@colocrm.com
authors.forename: M G
authors.initials: MG
authors.lastname: Katz-Jaffe
authors.forename: B R
authors.initials: BR
authors.lastname: McCallie
authors.forename: A
authors.initials: A
authors.lastname: Janesch
authors.forename: J A
authors.initials: JA
authors.lastname: Filipovits
authors.forename: W B
authors.initials: WB
authors.lastname: Schoolcraft
authors.forename: D K
authors.initials: DK
authors.lastname: Gardner
mesh_terms: D001755:Blastocyst / Q000378:metabolism*; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D011085:Polycystic Ovary Syndrome / Q000378:metabolism*; D020543:Proteome / Q000032:analysis*
chemical_list: D020543:Proteome