Antitumor activity of quaternized chitosan-based electrospun implants against Graffi myeloid tumor.
Nanofibrous implants containing quaternized chitosan (QCh), poly(L-lactide-co-D,L-lactide) (coPLA), and the antitumor drug doxorubicin (DOX) were fabricated by electrospinning. The surface chemical composition and the morphology of the implants were characterized by XPS and SEM. In vitro cell viability studies demonstrated that QCh- and DOX-based implants exhibited high cytotoxicity against Graffi tumor cells. The implants efficiently inhibited the growth of Graffi tumor in hamsters with minimum weight loss. Insertion of QCh/coPLA/DOX implants in the place of removed tumor led to an increase in the animal survival rate and to a decrease in the percentage of recurrences.
Toshkova
R
R
Institute of Experimental Pathology and Parasitology, Bulgarian Academy of Sciences, Acad. G. Bonchev St. bl. 25, BG-1113 Sofia, Bulgaria.
Manolova
N
N
Gardeva
E
E
Ignatova
M
M
Yossifova
L
L
Rashkov
I
I
Alexandrov
M
M
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000008:administration & dosage / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry*; D006224:Cricetinae; D004317:Doxorubicin / Q000008:administration & dosage / Q000494:pharmacology*; D004337:Drug Carriers / Q000737:chemistry*; D004343:Drug Implants; D007951:Leukemia, Myeloid / Q000188:drug therapy* / Q000473:pathology; D008647:Mesocricetus; D008855:Microscopy, Electron, Scanning; D057139:Nanofibers; D056951:Photoelectron Spectroscopy; D011091:Polyesters / Q000737:chemistry
D000903:Antibiotics, Antineoplastic; D004337:Drug Carriers; D004343:Drug Implants; D011091:Polyesters; C033616:poly(lactide); D004317:Doxorubicin; D048271:Chitosan