Selective and sensitive detection and quantification of arylamine N-acetyltransferase 2 by a ratiometric fluorescence probe.
A novel NAT2 specific probe has been developed for sensitive detection of the activity of NAT2. Upon NAT2 catalyzed acetylation of the molecule to N-acetyl-amonafide, the ratiometric response of fluorescence with significant changes in wavelength and intensity is observed. Its significant application potential has been successfully demonstrated in cellular systems.
Cui
Lei
L
State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of Chemical Biology, East China University of Science and Technology, Shanghai 200237, China.
Zhong
Ye
Y
Zhu
Weiping
W
Xu
Yufang
Y
Qian
Xuhong
X
D000107:Acetylation; D000225:Adenine; D001191:Arylamine N-Acetyltransferase / Q000032:analysis / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism*; D056945:Hep G2 Cells; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods; D053644:Naphthalimides / Q000737:chemistry / Q000378:metabolism*; D063065:Organophosphonates; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*
D005456:Fluorescent Dyes; D053644:Naphthalimides; D063065:Organophosphonates; C037020:amonafide; D001191:Arylamine N-Acetyltransferase; C478900:NAT2 protein, human; D000225:Adenine