Protective effects of platinum nanoparticles against UV-light-induced epidermal inflammation.
Intracellular reactive oxygen species (ROS) and apoptosis play important roles in the ultraviolet (UV)-induced inflammatory responses in the skin. Metal nanoparticles have been developed to increase the catalytic activity of metals, which is because of the large surface area of smaller particles. Platinum nanoparticles (nano-Pt) protected by poly acrylic acid were manufactured by reduction with ethanol. A marked increase in ROS production was observed in UV-treated HaCaT keratinocytes cell lines, while a decrease in ROS production was observed in nano-Pt-treated cells. Pretreatment of the cells with nano-Pt also caused a significant inhibition of UVB- and UVC-induced apoptosis. Furthermore, we found that mice treated with nano-Pt gel prior to UV irradiation showed significant inhibition of UVB-induced inflammation and UVA-induced photoallergy compared to UV-irradiated control mice. These results suggest that nano-Pt effectively protects against UV-induced inflammation by decreasing ROS production and inhibiting apoptosis in keratinocytes.
Yoshihisa
Yoko
Y
Department of Dermatology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Sugitani, Toyama, Japan.
Honda
Ayumi
A
Zhao
Qing-Li
QL
Makino
Teruhiko
T
Abe
Riichiro
R
Matsui
Kotaro
K
Shimizu
Hiroshi
H
Miyamoto
Yusei
Y
Kondo
Takashi
T
Shimizu
Tadamichi
T
D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000528:radiation effects; D053148:Caspase 3 / Q000378:metabolism; D002460:Cell Line; D016207:Cytokines / Q000378:metabolism; D017454:Dermatitis, Photoallergic / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*; D004431:Ear, External / Q000378:metabolism / Q000473:pathology; D024841:Fluoroquinolones / Q000008:administration & dosage / Q000494:pharmacology; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D008297:Male; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects / Q000528:radiation effects; D053768:Metal Nanoparticles / Q000008:administration & dosage / Q000737:chemistry / Q000627:therapeutic use*; D051379:Mice; D008807:Mice, Inbred BALB C; D010984:Platinum / Q000008:administration & dosage / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology / Q000627:therapeutic use*; D011855:Radiodermatitis / Q000473:pathology / Q000517:prevention & control*; D017382:Reactive Oxygen Species / Q000378:metabolism; D014466:Ultraviolet Rays; D051028:bcl-2-Associated X Protein / Q000378:metabolism; D051020:bcl-X Protein / Q000378:metabolism; D019014:fas Receptor / Q000378:metabolism
C494826:BAX protein, human; C494811:BCL2L1 protein, human; D016207:Cytokines; C491901:FAS protein, human; D024841:Fluoroquinolones; D017382:Reactive Oxygen Species; D051028:bcl-2-Associated X Protein; D051020:bcl-X Protein; D019014:fas Receptor; D010984:Platinum; D053148:Caspase 3; C053091:lomefloxacin