title: Markers of Blood Cell Activation and Complement Activation in Factor VIII and von Willebrand Factor Concentrates.
abstract: BACKGROUND: Preparations of commercially available clotting factor VIII are complex protein mixtures. Most of them contain either von Willebrand factor or human serum albumin as stabilizers. The aim of the study was to quantify further proteins in twelve concentrates either of recombinant origin or derived from human plasma. METHODS: Proteins were separated by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). Some proteins were quantified by ELISA. RESULTS: Recombinant clotting factor preparations showed fewer protein spots in the 2D-PAGE, than plasma-derived preparations. Proteins identified in some of the plasma-derived concentrates included up to 90 ng/IU of the anaphylatoxin C3a, up to 40 ng/IU of the platelet a-granule protein thrombospondin-1, up to 0.85 ng/IU of the platelet a-granule protein platelet factor 4, 3.5 ng/IU myeloperoxidase secreted by leukocytes and up to 0.05 ng/IU of the leukocyte-secreted protein a-defensin. The protein content differed between concentrates from different manufacturers. CONCLUSIONS: The origin of the plasma used to prepare the factor concentrates might influence the protein impurities in these products. It is unknown whether the impurities observed have long-term consequences for chronic inflammatory conditions.
authors.affiliation: Experimental und Clinical Hemostasis, Department of Anesthesiology and Intensive Care, University Hospital Muenster, Germany.
authors.forename: Martin F
authors.initials: MF
authors.lastname: Brodde
authors.forename: Beate E
authors.initials: BE
authors.lastname: Kehrel
references.citation: Cancer Metastasis Rev. 1992 Nov;11(3-4):313-24
references.pmid: 1384998
references.citation: FASEB J. 2003 Aug;17(11):1490-2
references.pmid: 12824298
references.citation: Inflamm Allergy Drug Targets. 2009 Jul;8(3):236-46
references.pmid: 19601884
references.citation: Br J Haematol. 1990 Sep;76(1):80-7
references.pmid: 2121265
references.citation: Dis Markers. 2008;25(2):123-9
references.pmid: 18957723
references.citation: Proc Natl Acad Sci U S A. 1990 Sep;87(17):6624-8
references.pmid: 1697685
references.citation: Atherosclerosis. 2007 Oct;194(2):452-7
references.pmid: 16989837
references.citation: Proc Natl Acad Sci U S A. 2008 Nov 25;105(47):18584-9
references.pmid: 19011099
references.citation: Br J Pharmacol. 2007 Nov;152(6):838-54
references.pmid: 17592500
references.citation: Nat Med. 2009 Jan;15(1):97-103
references.pmid: 19122657
references.citation: Immunol Rev. 2004 Apr;198:169-84
references.pmid: 15199962
references.citation: Blood Coagul Fibrinolysis. 1996 Mar;7(2):202-5
references.pmid: 8735819
references.citation: Circulation. 2006 Apr 18;113(15):1871-8
references.pmid: 16606792
references.citation: Am J Physiol Heart Circ Physiol. 2008 Nov;295(5):H1817-24
references.pmid: 18805897
references.citation: Electrophoresis. 2000 Apr;21(6):1037-53
references.pmid: 10786879
references.citation: Microvasc Res. 1997 Jul;54(1):13-26
references.pmid: 9245640
references.citation: N Engl J Med. 2007 Aug 9;357(6):535-44
references.pmid: 17687129
references.citation: Curr Vasc Pharmacol. 2010 May;8(3):338-43
references.pmid: 19485899
references.citation: Vox Sang. 1999;77 Suppl 1:90-9
references.pmid: 10529698
references.citation: Blood. 2006 Nov 15;108(10):3580-9
references.pmid: 16882710
references.citation: J Chromatogr A. 1999 Aug 6;852(1):175-88
references.pmid: 10480242
references.citation: Thromb Haemost. 2004 Feb;91(2):334-44
references.pmid: 14961162
references.citation: Br J Haematol. 2001 Jul;114(1):233-40
references.pmid: 11472374
references.citation: Mol Cell Proteomics. 2008 Jul;7(7):1204-13
references.pmid: 18349140
references.citation: Immunol Res. 2007;37(3):161-75
references.pmid: 17873401
references.citation: J Invest Dermatol. 1994 May;102(5):803-6
references.pmid: 7513741
references.citation: Transfus Med. 2004 Feb;14(1):45-52
references.pmid: 15043593
references.citation: J Thromb Haemost. 2009 May;7(5):780-6
references.pmid: 19220727
references.citation: J Immunol. 2001 Feb 1;166(3):2025-32
references.pmid: 11160252
references.citation: Electrophoresis. 2009 Oct;30(20):3636-46
references.pmid: 19768705
references.citation: Blood. 1988 Apr;71(4):1074-8
references.pmid: 3355888
references.citation: J Lipid Res. 2009 Apr;50 Suppl:S346-51
references.pmid: 19091698
references.citation: Int J Mol Med. 2003 Dec;12(6):995-1001
references.pmid: 14612980
references.citation: Nat Rev Cancer. 2009 Mar;9(3):182-94
references.pmid: 19194382
references.citation: Clin Sci (Lond). 2009 May;116(9):681-95
references.pmid: 19323651
references.citation: Acta Med Scand Suppl. 1962;379:61-110
references.pmid: 14479888
references.citation: Nature. 2000 Aug 31;406(6799):998-1001
references.pmid: 10984054
references.citation: Arterioscler Thromb Vasc Biol. 1997 Oct;17(10):2107-14
references.pmid: 9351379
references.citation: Transfus Med. 2002 Oct;12(5):317-24
references.pmid: 12383338
references.citation: J Leukoc Biol. 2008 Apr;83(4):936-45
references.pmid: 18203873
references.citation: Nat Med. 2007 Oct;13(10):1176-84
references.pmid: 17828273
references.citation: Curr Top Microbiol Immunol. 2006;306:27-66
references.pmid: 16909917
references.citation: J Clin Invest. 2008 Oct;118(10):3491-502
references.pmid: 18787642
references.citation: Crit Rev Immunol. 2008;28(3):185-200
references.pmid: 19024344
references.citation: Transfusion. 2005 Mar;45(3):427-32
references.pmid: 15752162
references.citation: Arterioscler Thromb Vasc Biol. 2008 Nov;28(11):1920-7
references.pmid: 18723831
references.citation: J Exp Med. 2001 Jun 18;193(12):1341-9
references.pmid: 11413189
references.citation: Haemophilia. 2005 Jul;11(4):319-25
references.pmid: 16011582
references.citation: J Clin Invest. 1979 Sep;64(3):804-11
references.pmid: 89118
references.citation: Vox Sang. 2005 Jan;88(1):17-21
references.pmid: 15663718
references.citation: Ann Hematol. 1991 Sep;63(3):145-51
references.pmid: 1932290
references.citation: Diabetes. 2009 Sep;58(9):2006-17
references.pmid: 19581423
references.citation: Transfus Apher Sci. 2008 Aug;39(1):69-74
references.pmid: 18583192
references.citation: Proc Natl Acad Sci U S A. 2005 Sep 13;102(37):13141-6
references.pmid: 16150726