title: Comparison of dog and rabbit plasmas in the tube coagulase test for Staphylococcus aureus.
abstract: The tube coagulase test, an invaluable laboratory tool for identifying Staphylococcus aureus, is most often done using rabbit plasma. However, there is evidence that depending on the origin of the isolates, other plasmas may be superior. The current study sought to compare the utility of dog and rabbit plasma in the coagulase test for S. aureus isolated from canine (n = 28), bovine (n = 29), and human (n = 30) hosts. Overall, coagulation times were significantly faster for dog (2.38 hr) than rabbit (3.19 hr) plasma. When coagulation times were compared by isolate origin, no significant differences were found for rabbit plasma, whereas bovine isolates clotted dog plasma significantly faster (1.86 hr) than canine (2.79 hr) or human (2.38 hr) isolates. Investigators should be aware that rabbit plasma may not be the ideal coagulase-testing medium for S. aureus from all sources.
authors.affiliation: Department of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Canada. joe.rubin@usask.ca
authors.forename: Joseph Elliot
authors.initials: JE
authors.lastname: Rubin
authors.forename: Melanie Kirsten
authors.initials: MK
authors.lastname: Bayly
authors.forename: Manuel
authors.initials: M
authors.lastname: Chirino-Trejo
mesh_terms: D000818:Animals; D001777:Blood Coagulation; D002417:Cattle; D003030:Coagulase / Q000097:blood*; D004285:Dogs / Q000097:blood*; D006801:Humans; D016106:Methicillin Resistance; D011817:Rabbits / Q000097:blood*; D013045:Species Specificity; D013203:Staphylococcal Infections / Q000097:blood / Q000453:epidemiology / Q000662:veterinary*; D013211:Staphylococcus aureus / Q000145:classification / Q000201:enzymology / Q000302:isolation & purification*
chemical_list: D003030:Coagulase