Metabolic labeling of glycoconjugates with photocrosslinking sugars.
Protein-carbohydrate interactions play essential roles in a variety of biological processes. This class of interactions is particularly important in development, immunology, infection, and carcinogenesis. However, the transient nature of glycan-dependent interactions hampers efforts to detect and characterize these complexes. Photocrosslinking is emerging as a powerful tool to discover and study glycan-dependent complexes. Through the use of photocrosslinking groups, UV irradiation can be employed to introduce a covalent bond between two transiently interacting molecules. Here we describe the use of metabolic oligosaccharide engineering to incorporate a photocrosslinkable sugar into cellular glycoconjugates and the use of this photocrosslinker to covalently capture glycan-mediated interactions.
Yu
Seok-Ho
SH
Division of Translational Research, Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, USA.
Bond
Michelle R
MR
Whitman
Chad M
CM
Kohler
Jennifer J
JJ
D002240:Carbohydrate Sequence; D002478:Cells, Cultured; D003432:Cross-Linking Reagents / Q000737:chemistry*; D006001:Glycoconjugates / Q000737:chemistry*; D006801:Humans; D019169:Jurkat Cells; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D019158:N-Acetylneuraminic Acid / Q000737:chemistry; D019920:Photoaffinity Labels / Q000737:chemistry / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry*; D015202:Protein Engineering / Q000379:methods
D003432:Cross-Linking Reagents; D006001:Glycoconjugates; D008562:Membrane Glycoproteins; D019920:Photoaffinity Labels; D011134:Polysaccharides; D019158:N-Acetylneuraminic Acid