Lack of protection of pre-immunization with saliva of long-term colonized Phlebotomus papatasi against experimental challenge with Leishmania major and saliva of wild-caught P. papatasi.
Immunity to saliva of Phlebotomus papatasi protects against Leishmania major infection as determined by co-inoculation of parasites with salivary gland homogenates (SGHs) of this vector. These results were obtained with long-term colonized female P. papatasi. We investigated the effect of pre-immunization with SGH of long-term colonized P. papatasi against L. major infection co-inoculated with SGH of wild-caught P. papatasi. Our results showed that pre-exposure to SGH of long-term, colonized P. papatasi do not confer protection against infection with L. major co-inoculated with SGH of wild-caught P. papatasi. These preliminary results strongly suggest that the effectiveness of a vector saliva-based vaccine derived from colonized sand fly populations may be affected by inconsistent immune response after natural exposure.
Ahmed
Sami Ben Hadj
SB
Laboratory of Vector Ecology, Laboratory of Epidemiology and Ecology of Parasites, and Laboratory of Immuno-Pathology, Vaccinology, and Molecular Genetics, Institut Pasteur de Tunis, Tunis, Tunisia.
Kaabi
Belhassen
B
Chelbi
Ifhem
I
Derbali
Mohamed
M
Cherni
Saifedine
S
Laouini
Dhafer
D
Zhioua
Elyes
E
D000818:Animals; D005260:Female; D018320:Leishmania major / Q000276:immunology*; D010691:Phlebotomus / Q000276:immunology*; D012463:Saliva
J Med Entomol. 2010 Jan;47(1):74-9
20180311
Am J Trop Med Hyg. 1984 Jul;33(4):690-4
6476217
J Med Entomol. 1993 Mar;30(2):407-13
8459418
Parasitol Int. 2009 Sep;58(3):220-6
19454323
Proc Natl Acad Sci U S A. 2008 Jun 3;105(22):7845-50
18509051
Scand J Immunol. 2009 Oct;70(4):389-95
19751274
Insect Mol Biol. 1999 May;8(2):267-75
10380110
Science. 2000 Nov 17;290(5495):1351-4
11082061
Am J Trop Med Hyg. 1997 Aug;57(2):216-21
9288819
PLoS Negl Trop Dis. 2008 Apr 16;2(4):e226
18414648
J Exp Med. 2001 Aug 6;194(3):331-42
11489952
Biometrics. 1982 Dec;38(4):963-74
7168798
J Immunol. 2001 Nov 1;167(9):5226-30
11673536
Insect Mol Biol. 2005 Apr;14(2):145-50
15796747
Am J Trop Med Hyg. 2004 Mar;70(3):286-93
15031518
J Exp Med. 1998 Nov 16;188(10):1941-53
9815271
J Med Entomol. 2007 Jul;44(4):597-600
17695013
J Med Entomol. 2007 Mar;44(2):385-8
17427714