[Genetic characteristics of Haemophilus influenzae type b isolated from children with bacterial meningitis in Moscow in 2007-2009].
OBJECTIVE
Genetic characterization of 37 strains and CSF samples containing DNA of Haemophilus influenzae type b isolated in Moscow during 2007-2009.


METHODS
Multilocus sequence-typing method was used and also variant of method for capsule type determination was approbated.


RESULTS
Ten sequence types, of which 7 were described in previous studies and 3 were revealed for the first time during this work, were detected in studied sample. ST-6 and ST-92 were the most frequently detected--9 strains (24%) of Hib belonging to each sequence type were revealed. All detected sequence types, except one, belong to clonal complex "ST-6" ("A1/A2"). Obtained data were compared with results of typing of Hib strains isolated in Moscow in 1999-2001. Genetic changes in studied population of Hib are characterized by decreased proportion of Hib belonging to ST-6 (from 54% to 24%) and increased number of sequence types belonging to clonal complex "ST-6" differing from ST-6 on more than one locus of allelic profile (from 2 types [2 strains, 5.4%] to 5 types [9 strains, 24%]).


CONCLUSIONS
In 2007-2009, number of Hib strains with sequence type ST-95 (7 strains, 19%), which is typical for strains circulating in Russia, is markedly increased. Capsule type I was detected in 32 (86.5%) of studied strains, whereas capsule type II--in 5 (13.5%) of studied strains. Capsule type II was detected only in Hib strains with ST-80 sequence type.
Mironov
K O
KO
Tagachenkova
T A
TA
Platonov
A E
AE
Iakovenko
M L
ML
Koroleva
I S
IS
Shipulin
G A
GA
D000483:Alleles; D016667:Bacterial Capsules / Q000145:classification; D002648:Child; D002675:Child, Preschool; D004269:DNA, Bacterial / Q000235:genetics; D019741:Haemophilus influenzae type b / Q000235:genetics*; D006801:Humans; D007223:Infant; D008583:Meningitis, Haemophilus / Q000453:epidemiology* / Q000382:microbiology*; D009031:Moscow / Q000453:epidemiology; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial