Inherited adaptation of genome-rewired cells in response to a challenging environment.
Despite their evolutionary significance, little is known about the adaptation dynamics of genomically rewired cells in evolution. We have confronted yeast cells carrying a rewired regulatory circuit with a severe and unforeseen challenge. The essential HIS3 gene from the histidine biosynthesis pathway was placed under the exclusive regulation of the galactose utilization system. Glucose containing medium strongly represses the GAL genes including HIS3 and these rewired cells are required to operate this essential gene. We show here that although there were no adapted cells prior to the encounter with glucose, a large fraction of cells adapted to grow in this medium and this adaptation was stably inherited. The adaptation relied on individual cells that switched into an adapted state and, thus, the adaptation was due to a response of many individual cells to the change in environment and not due to selection of rare advantageous phenotypes. The adaptation of numerous individual cells by heritable phenotypic switching in response to a challenge extends the common evolutionary framework and attests to the adaptive potential of regulatory circuits.
David
Lior
L
Stolovicki
Elad
E
Haziz
Efrat
E
Braun
Erez
E
Nature. 1983 Apr 7;302(5908):495-500
6339947
Mol Gen Genet. 1998 Jun;258(5):546-52
9669337
Science. 1975 Apr 11;188(4184):107-16
1090005
Nature. 1998 Nov 26;396(6709):336-42
9845070
Genetics. 1992 Sep;132(1):9-21
1398066
Evolution. 2008 Sep;62(9):2155-77
18616572
Proc Natl Acad Sci U S A. 1994 Jul 19;91(15):6808-14
8041701
Genetics. 1991 Aug;128(4):695-701
1916241
Nature. 2002 Jun 6;417(6889):618-24
12050657
Crit Rev Biochem Mol Biol. 2007 Jul-Aug;42(4):285-311
17687670
Genetics. 1998 Apr;148(4):1667-86
9560386
Genome Res. 2005 Oct;15(10):1365-72
16204189
PLoS Biol. 2008 Feb;6(2):e38
18303948
Science. 2008 Mar 28;319(5871):1797-9
18369141
Science. 2004 Sep 10;305(5690):1622-5
15308767
Mol Syst Biol. 2007;3:106
17453047
Nat Rev Genet. 2007 Mar;8(3):206-16
17304246
Nature. 1988 Sep 8;335(6186):142-5
3045565
Nat Rev Genet. 2009 Feb;10(2):141-8
19139764
Annu Rev Microbiol. 2006;60:477-501
16761951
Trends Genet. 2007 Aug;23(8):396-402
17566592
Science. 2007 Aug 10;317(5839):813-5
17690297
Nat Rev Microbiol. 2006 Jul;4(7):556-62
16778840
Nat Rev Genet. 2003 Jun;4(6):457-69
12776215
Nat Rev Genet. 2001 Jul;2(7):504-15
11433357
Genetics. 1943 Nov;28(6):491-511
17247100
Genetics. 2006 May;173(1):75-85
16510783
J Theor Biol. 1996 Jul 7;181(1):1-9
8796186
PLoS Biol. 2007 Apr;5(4):e81
17373856
Curr Opin Microbiol. 2001 Oct;4(5):570-81
11587935
Q Rev Biol. 2009 Jun;84(2):131-76
19606595
Genetica. 1998;102-103(1-6):109-25
9720275
Proc Natl Acad Sci U S A. 1950 Dec;36(12):708-19
14808160
Nat Rev Genet. 2005 Sep;6(9):709-15
16094311
Science. 2006 Jul 28;313(5786):514-7
16825532
PLoS Biol. 2005 Jul;3(7):e245
16000021
Curr Genet. 1999 Mar;35(2):77-81
10079325
Mol Biol Evol. 1998 Jan;15(1):1-5
9491599