Highly sensitive and selective odorant sensor using living cells expressing insect olfactory receptors.
This paper describes a highly sensitive and selective chemical sensor using living cells (Xenopus laevis oocytes) within a portable fluidic device. We constructed an odorant sensor whose sensitivity is a few parts per billion in solution and can simultaneously distinguish different types of chemicals that have only a slight difference in double bond isomerism or functional group such as -OH, -CHO and -C(=O)-. We developed a semiautomatic method to install cells to the fluidic device and achieved stable and reproducible odorant sensing. In addition, we found that the sensor worked for multiple-target chemicals and can be integrated with a robotic system without any noise reduction systems. Our developed sensor is compact and easy to replace in the system. We believe that the sensor can potentially be incorporated into a portable system for monitoring environmental and physical conditions.
Misawa
Nobuo
N
Institute of Industrial Science, University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan.
Mitsuno
Hidefumi
H
Kanzaki
Ryohei
R
Takeuchi
Shoji
S
D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D015153:Blotting, Western; D004566:Electrodes; D005260:Female; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D008564:Membrane Potentials / Q000502:physiology; D009865:Oocytes / Q000378:metabolism / Q000502:physiology*; D018035:Receptors, Odorant / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D015203:Reproducibility of Results; D014982:Xenopus laevis
D019476:Insect Proteins; D018035:Receptors, Odorant
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