[Comparison of efficacy of tests for differentiation of typical and atypical strains of Yersinia pestis and Yersinia pseudotuberculosis].
OBJECTIVE
To characterize species specificity of officially recommended tests for differentiation of Yersiniapestis and Yersinia pseudotuberculosis and propose additional tests allowing for more accurate identification.


METHODS
Natural, laboratory and typical strains oftwo Yersinia species were studied using microbiological, molecular and biochemical methods. For PCR species-specific primers complementary to certain fragments of chromosomal DNA of each species as well as to several plasmid genes of Y. pestis were used.


RESULTS
It was shown that such attributes of Y. pestis as form of colonies, fermentation ofrhamnose, melibiose and urea, susceptibility to diagnostic phages, nutritional requirements could be lost in pestis bacterial species or detected in pseudotuberculosis species. Such attribute as mobility as well as positive result of CoA-reaction on fraction V antigen are more reliable.


CONCLUSIONS
Guaranteed differentiation of typical and changed according to differential tests strains is provided only by PCR-analysis with primers vlml2for/ISrev216 and JS respectively, which are homologous to certain chromosome fragments of one of two Yersinia species.
Arsen'eva
T E
TE
Lebedeva
S A
SA
Trukhachev
A L
AL
Vasil'eva
E A
EA
Ivanova
V S
VS
Bozhko
N V
NV
D002876:Chromosomes, Bacterial / Q000235:genetics; D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics; D003937:Diagnosis, Differential; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D006801:Humans; D008553:Melibiose / Q000378:metabolism; D010930:Plague / Q000175:diagnosis*; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D012210:Rhamnose / Q000378:metabolism; D012680:Sensitivity and Specificity; D014508:Urea / Q000378:metabolism; D015010:Yersinia pestis / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D015011:Yersinia pseudotuberculosis / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D015012:Yersinia pseudotuberculosis Infections / Q000175:diagnosis*
D017931:DNA Primers; D004269:DNA, Bacterial; D014508:Urea; D008553:Melibiose; D012210:Rhamnose