Detection of metallo-β-lactamase genes in clinical specimens by a commercial multiplex PCR system.
hyplex®-MBL ID Multiplex PCR-ELISA, a novel method for identifying metallo-β-lactamase genes directly in clinical specimens, was evaluated using a consecutive collection of 326 samples from three hospitals in Greece characterized by high prevalence of VIM producers. The method exhibited high sensitivity (98.0%) and specificity (98.6%) and was proven reliable in detecting bla(VIM) genes in blood, urine, pus, and sputum samples that, as confirmed by conventional methods, contained various VIM-producing species. Future multicenter studies should be considered for the thorough evaluation of this method and its potential diagnostic utility.
Avlami
Athina
A
Department of Microbiology, Laikon General Hospital, Athens, Greece.
Bekris
S
S
Ganteris
G
G
Kraniotaki
Eleni
E
Malamou-Lada
Eleni
E
Orfanidou
Maria
M
Paniara
Olga
O
Pantazatou
Angeliki
A
Papagiannitsis
C C
CC
Platsouka
Evangelia
E
Stefanou
Ioanna
I
Tzelepi
Eva
E
Vagiakou
Helen
H
Miriagou
Vivi
V
D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006090:Gram-Negative Bacteria / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology*; D006115:Greece; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D001618:beta-Lactamases / Q000096:biosynthesis / Q000235:genetics*
D001426:Bacterial Proteins; D001618:beta-Lactamases