title: Effect of Curcuma comosa and estradiol on the spatial memory and hippocampal estrogen receptor in the post-training ovariectomized rats.
abstract: Curcuma comosa was reported to have uterotrophic effects in ovariectomized (OVX) rats. The present study aims to investigate the plant estrogenic-like effects of C. comosa hexane extract on spatial memory and estrogen receptors (ER) in the hippocampus. Female rats received 5 days' Morris water maze training before the ovariectomy, followed by the daily intraperitoneal administration of C. comosa hexane extract at a dose of 250 and 500 mg/kg body weight for 4 weeks. Estradiol (10 μg/kg body weight) was used as the positive control. OVX significantly increased the latency of the animal to the platform, while estradiol and both doses of C. comosa hexane extract reversed the memory impairments. By using the reverse transcription polymerase chain reaction technique, C. comosa hexane extract was shown to produce a selective increase of the ER alpha subtype but not beta subtype in hippocampus, while estradiol increased both of them. C. comosa did not affect spatial memory, learning ability, and ER mRNA levels in the sham OVX rats. In conclusion, C. comosa hexane extract improved the spatial memory of the OVX rats, which was similar to estrogen. The influence on the memory changes might be selectively related to ER alpha in the hippocampus.
authors.affiliation: Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand.
authors.forename: Jian
authors.initials: J
authors.lastname: Su
authors.forename: Bungorn
authors.initials: B
authors.lastname: Sripanidkulchai
authors.forename: Kittisak
authors.initials: K
authors.lastname: Sripanidkulchai
authors.forename: Pawinee
authors.initials: P
authors.lastname: Piyachaturawat
authors.forename: Nawarat
authors.initials: N
authors.lastname: Wara-Aswapati
mesh_terms: D000818:Animals; D030024:Curcuma / Q000737:chemistry*; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism*; D018782:Maze Learning / Q000187:drug effects; D008568:Memory / Q000187:drug effects*; D010052:Ovariectomy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D011960:Receptors, Estrogen / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D010936:Plant Extracts; D011960:Receptors, Estrogen; D004958:Estradiol
references.citation: J Neurosci. 1999 Aug 1;19(15):6385-93
references.pmid: 10414967
references.citation: Anat Sci Int. 2003 Jun;78(2):85-97
references.pmid: 12828421
references.citation: Brain Res Brain Res Protoc. 2003 Mar;11(1):9-18
references.pmid: 12697258
references.citation: Annu Rev Plant Biol. 2004;55:225-61
references.pmid: 15377220
references.citation: Gend Med. 2005 Mar;2(1):41-56
references.pmid: 16115597
references.citation: Endocrinology. 2004 Jul;145(7):3055-61
references.pmid: 14976146
references.citation: Neurobiol Learn Mem. 2003 Nov;80(3):315-22
references.pmid: 14521873
references.citation: J Steroid Biochem Mol Biol. 1998 Aug;66(3):113-20
references.pmid: 9719445
references.citation: J Neurobiol. 1998 Sep 5;36(3):357-78
references.pmid: 9733072
references.citation: Neurobiol Learn Mem. 2004 Sep;82(2):142-9
references.pmid: 15341799
references.citation: J Natl Cancer Inst. 2004 Dec 15;96(24):1856-65
references.pmid: 15601642
references.citation: Brain Res. 2006 Dec 18;1126(1):176-82
references.pmid: 16934233
references.citation: Endocrinology. 2004 Aug;145(8):3658-70
references.pmid: 15087431
references.citation: Endocrinology. 2006 Jan;147(1):607-14
references.pmid: 16239296
references.citation: Brain Res. 2000 Nov 17;883(2):258-64
references.pmid: 11074057
references.citation: Proc Natl Acad Sci U S A. 2002 Mar 19;99(6):3996-4001
references.pmid: 11891272
references.citation: Endocrinology. 1993 Aug;133(2):431-2
references.pmid: 8344187
references.citation: Brain Res. 2005 Aug 9;1052(2):163-73
references.pmid: 16023091
references.citation: Endocrinology. 2003 May;144(5):2055-67
references.pmid: 12697714
references.citation: Cardiovasc Res. 2005 May 1;66(2):295-306
references.pmid: 15820198
references.citation: Clin Endocrinol (Oxf). 2005 Jul;63(1):50-5
references.pmid: 15963061
references.citation: Neurobiol Learn Mem. 2006 Nov;86(3):336-43
references.pmid: 16979357
references.citation: Mol Aspects Med. 2006 Aug;27(4):299-402
references.pmid: 16914190
references.citation: Arch Intern Med. 2001 May 14;161(9):1161-72
references.pmid: 11343439