Enhancement of targeted homologous recombination in Mycoplasma mycoides subsp. capri by inclusion of heterologous recA.
A suicide plasmid, pExp1-ctpA::tetM-recAec, employing recA from Escherichia coli and tetM as a selection marker, was used to generate ctpA knockout mutants in Mycoplasma mycoides subsp. capri through targeted gene disruption. Inclusion of E. coli recA greatly enhanced both the consistency and the recovery of mutants generated by homologous recombination.
Allam
Ayman B
AB
Department of Infectious Diseases and Pathology, College of Veterinary Medicine, University of Florida, Gainesville, FL, USA.
Reyes
Leticia
L
Assad-Garcia
Nacyra
N
Glass
John I
JI
Brown
Mary B
MB
D020418:Algal Proteins; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002268:Carboxypeptidases / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D055786:Gene Knockout Techniques; D018390:Gene Targeting / Q000379:methods*; D005822:Genetic Vectors; D009176:Mycoplasma mycoides / Q000235:genetics*; D010957:Plasmids; D043484:Proprotein Convertases / Q000235:genetics; D011938:Rec A Recombinases / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D011995:Recombination, Genetic; D012641:Selection, Genetic; D013753:Tetracycline Resistance
D020418:Algal Proteins; D001426:Bacterial Proteins; D011994:Recombinant Proteins; C083138:Tet M resistance protein, Bacteria; D011938:Rec A Recombinases; D002268:Carboxypeptidases; D043484:Proprotein Convertases; C073491:photosystem II, D1 polypeptide carboxy-terminal processing protease
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