title: Catalpol increases brain angiogenesis and up-regulates VEGF and EPO in the rat after permanent middle cerebral artery occlusion.
abstract: To investigate the role and mechanism of catalpol in brain angiogenesis in a rat model of stroke, the effect of catalpol (5 mg/kg; i.p) or vehicle administered 24 hours after permanent middle cerebral artery occlusion (pMCAO) on behavior, angiogenesis, ultra-structural integrity of brain capillary endothelial cells, and expression of EPO and VEGF were assessed. Repeated treatments with Catalpol reduced neurological deficits and significantly improved angiogenesis, while significantly increasing brain levels of EPO and VEGF without worsening BBB edema. These results suggested that catalpol might contribute to infarcted-brain angiogenesis and ameliorate the edema of brain capillary endothelial cells (BCECs) by upregulating VEGF and EPO coordinately.
authors.affiliation: School of Pharmaceutical Sciences & School of Chinese Medicine, Southwest University, Chongqing 400715, China. zhfbsci@yahoo.cn
authors.forename: Hui-Feng
authors.initials: HF
authors.lastname: Zhu
authors.forename: Dong
authors.initials: D
authors.lastname: Wan
authors.forename: Yong
authors.initials: Y
authors.lastname: Luo
authors.forename: Jia-Li
authors.initials: JL
authors.lastname: Zhou
authors.forename: Li
authors.initials: L
authors.lastname: Chen
authors.forename: Xiao-Yu
authors.initials: XY
authors.lastname: Xu
mesh_terms: D000818:Animals; D015153:Blotting, Western; D042783:Endothelial Cells / Q000187:drug effects; D004921:Erythropoietin / Q000235:genetics / Q000378:metabolism*; D006321:Heart / Q000187:drug effects / Q000503:physiopathology*; D007150:Immunohistochemistry; D020244:Infarction, Middle Cerebral Artery / Q000378:metabolism / Q000503:physiopathology*; D057888:Iridoid Glucosides / Q000494:pharmacology*; D008297:Male; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D011597:Psychomotor Performance / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D015854:Up-Regulation; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism*
chemical_list: D057888:Iridoid Glucosides; D042461:Vascular Endothelial Growth Factor A; D004921:Erythropoietin; C078040:catalpol
references.citation: Nat Med. 2006 Jul;12(7):793-800
references.pmid: 16799557
references.citation: J Clin Invest. 2000 Oct;106(7):829-38
references.pmid: 11018070
references.citation: Neuroreport. 2003 Jun 11;14(8):1171-6
references.pmid: 12821803
references.citation: Mol Cell Neurosci. 2005 Aug;29(4):569-79
references.pmid: 15936213
references.citation: J Neurosci Res. 2007 Mar;85(4):740-7
references.pmid: 17243175
references.citation: Stroke. 1994 Sep;25(9):1794-8
references.pmid: 7521076
references.citation: Brain Res. 2006 Oct 18;1115(1):179-85
references.pmid: 16928364
references.citation: J Neurobiol. 2006 Feb 15;66(3):236-42
references.pmid: 16329123
references.citation: J Cereb Blood Flow Metab. 1999 Jun;19(6):643-51
references.pmid: 10366194
references.citation: Exp Neurol. 2007 Mar;204(1):283-7
references.pmid: 17188266
references.citation: JAMA. 2001 Dec 5;286(21):2673-82
references.pmid: 11730442
references.citation: Patol Pol. 1993;44(4):203-9
references.pmid: 7508594
references.citation: Biochem Biophys Res Commun. 2003 Nov 28;311(4):809-14
references.pmid: 14623253
references.citation: Pharmacol Rev. 2005 Jun;57(2):173-85
references.pmid: 15914466
references.citation: Neuron. 2002 Jun 13;34(6):945-60
references.pmid: 12086642
references.citation: Proc Natl Acad Sci U S A. 1990 Aug;87(15):5978-82
references.pmid: 2165612
references.citation: Pediatr Res. 2007 Jun;61(6):671-5
references.pmid: 17426655
references.citation: Brain Res. 2004 Dec 17;1029(2):179-85
references.pmid: 15542072
references.citation: Stroke. 2004 Feb;35(2):354-6
references.pmid: 14757877
references.citation: Int J Biol Sci. 2010 Jul 04;6(4):396-406
references.pmid: 20616880
references.citation: Minerva Chir. 2002 Aug;57(4):437-48
references.pmid: 12145573
references.citation: Neurosci Res. 2004 Oct;50(2):169-77
references.pmid: 15380324
references.citation: J Cereb Blood Flow Metab. 2004 Jun;24(6):693-702
references.pmid: 15181377
references.citation: Biochem Biophys Res Commun. 2003 Oct 24;310(3):804-10
references.pmid: 14550275
references.citation: Stroke. 1986 May-Jun;17(3):472-6
references.pmid: 3715945
references.citation: Biochim Biophys Acta. 2010 Jan;1802(1):80-91
references.pmid: 19751827
references.citation: Nat Rev Neurosci. 2004 May;5(5):347-60
references.pmid: 15100718
references.citation: J Exp Biol. 2004 Aug;207(Pt 18):3233-42
references.pmid: 15299044
references.citation: Biochem Biophys Res Commun. 2005 Apr 29;330(1):46-52
references.pmid: 15781230
references.citation: Brain. 2005 Jan;128(Pt 1):52-63
references.pmid: 15509618
references.citation: Microvasc Res. 2002 Sep;64(2):326-33
references.pmid: 12204656
references.citation: J Appl Physiol (1985). 2006 Jan;100(1):328-35
references.pmid: 16357086
references.citation: Eur J Neurosci. 2003 Nov;18(9):2538-44
references.pmid: 14622154
references.citation: Brain Res. 2006 Dec 6;1123(1):68-79
references.pmid: 17078935
references.citation: Mech Dev. 2001 Oct;108(1-2):45-57
references.pmid: 11578860
references.citation: J Cereb Blood Flow Metab. 2002 Apr;22(4):379-92
references.pmid: 11919509
references.citation: Circ Res. 2007 Mar 16;100(5):662-9
references.pmid: 17293480
references.citation: Eur Neurol. 2006;56(2):106-12
references.pmid: 16960450
references.citation: Pediatr Res. 2005 Apr;57(4):481-7
references.pmid: 15718373
references.citation: Toxicon. 2005 Dec 15;46(8):845-51
references.pmid: 16269165
references.citation: Proc Natl Acad Sci U S A. 2000 Sep 12;97(19):10526-31
references.pmid: 10984541
references.citation: Int Immunopharmacol. 2010 Apr;10(4):493-9
references.pmid: 20123040
references.citation: Oncogene. 2000 Apr 20;19(17):2138-46
references.pmid: 10815805
references.citation: Zhongguo Zhong Yao Za Zhi. 2009 Jul;34(13):1717-9
references.pmid: 19873789
references.citation: Proc Natl Acad Sci U S A. 2002 Jun 25;99(13):9031-6
references.pmid: 12084941
references.citation: Brain Res. 2005 Feb 9;1034(1-2):34-9
references.pmid: 15713257
references.citation: Brain Res. 2003 May 16;972(1-2):31-7
references.pmid: 12711075
references.citation: Brain Res. 2005 Jul 5;1049(1):95-103
references.pmid: 15935998
references.citation: Biochem Biophys Res Commun. 2007 Jan 26;352(4):889-95
references.pmid: 17157815
references.citation: Eur J Neurosci. 2004 Mar;19(6):1494-504
references.pmid: 15066146
references.citation: N Engl J Med. 2008 Sep 25;359(13):1317-29
references.pmid: 18815396