Diazirine-containing RNA photocrosslinking probes for the study of siRNA-protein interactions.
We here report the synthesis and characterization of small interfering RNAs with aryl trifluoromethyl diazirine moieties in the 3'-overhang regions, which allow sensitive detection of interacting proteins during assembly of the effector ribonucleoprotein complex by irradiation with minimally destructive long-wavelength ultraviolet light.
Kuboe
Satoru
S
Department of Biomolecular Science, Faculty of Engineering, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
Yoda
Mayuko
M
Ogata
Aya
A
Kitade
Yukio
Y
Tomari
Yukihide
Y
Ueno
Yoshihito
Y
D001389:Azirines / Q000737:chemistry*; D001483:Base Sequence; D010777:Photochemistry; D012313:RNA / Q000737:chemistry*; D034741:RNA, Small Interfering; D012689:Sequence Homology, Nucleic Acid; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D001389:Azirines; D034741:RNA, Small Interfering; D012313:RNA