Automating a 96-well microtiter plate assay for identification of AGEs inhibitors or inducers: application to the screening of a small natural compounds library.
Advanced glycation end-products (AGEs) are involved in the pathogenesis of numerous affections such as diabetes and neurological diseases. AGEs are also implied in various changes in tissues and organs. Therefore, compounds able to break them or inhibit their formation may be considered as potential drugs, dietary supplements, or bioactive additives. In this study, we have developed a rapid and reliable (Z' factor calculation) anti-AGEs activity screening based on the overall fluorescence of AGEs. This method was successfully evaluated on known AGEs inhibitors and on a small library of natural compounds, yielding coherent results when compared with literature data.
Derbré
Séverine
S
Laboratoire des Substances d'Origine Naturelle et Analogues Structuraux, UPRES-EA 921, IFR 149 QUASAV, UFR des Sciences pharmaceutiques et ingénierie de la santé, Université d'Angers, 16 Bd Daviers, 49045 Angers Cedex, France. severine.derbre@univ-angers.fr
Gatto
Julia
J
Pelleray
Aude
A
Coulon
Laurie
L
Séraphin
Denis
D
Richomme
Pascal
P
D001331:Automation; D004353:Drug Evaluation, Preclinical / Q000295:instrumentation / Q000379:methods*; D005453:Fluorescence; D017127:Glycation End Products, Advanced / Q000819:agonists* / Q000037:antagonists & inhibitors* / Q000737:chemistry; D054852:Small Molecule Libraries / Q000737:chemistry*
D017127:Glycation End Products, Advanced; D054852:Small Molecule Libraries