title: Design of a standalone-type beryllium vessel for high-pressure protein crystallography.
abstract: A standalone-type beryllium (Be) high-pressure crystallography vessel has been developed. Using a coupler-joint unit and a pressure valve, we could keep the pressure in the vessel constant at 100+/-1 MPa for more than 24 h without connecting to a pressure-generating apparatus. Diffraction spots of a glucose isomerase (GI) crystal under 100 MPa were collected using the vessel and a rotating copper-anode in-house x-ray generator (0.8 kW). We successfully collected a 2.0 A resolution data set of a 0.5 mm size GI crystal in an aqueous solution at 100 MPa.
authors.affiliation: Department of Life System, Institute of Technology and Science, The University of Tokushima, 2-1 Minamijosanjima, Tokushima 770-8506, Japan. suzuki@chem.tokushima-u.ac.jp
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Suzuki
authors.forename: Masayuki
authors.initials: M
authors.lastname: Tsukamoto
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Sakuraba
authors.forename: Masamitsu
authors.initials: M
authors.lastname: Matsumoto
authors.forename: Makoto
authors.initials: M
authors.lastname: Nagasawa
authors.forename: Katsuhiro
authors.initials: K
authors.lastname: Tamura
mesh_terms: D001608:Beryllium; D003461:Crystallography / Q000295:instrumentation*; D018360:Crystallography, X-Ray; D004867:Equipment Design / Q000379:methods*; D011312:Pressure; D011506:Proteins / Q000737:chemistry*
chemical_list: D011506:Proteins; D001608:Beryllium