title: Metabolic changes reveal the development of schistosomiasis in mice.
abstract: Schistosomiasis is a parasitic zoonosis caused by small trematode worms called schistosomes, amongst which Schistosoma japonicum (S. japonicum) is endemic in Asia. In order to understand the schistosome-induced changes in the host metabolism so as to facilitate early diagnosis of schistosomiasis, we systematically investigated the dynamic metabolic responses of mice biofluids and liver tissues to S. japonicum infection for five weeks using (1)H NMR spectroscopy in conjunction with multivariate data analysis. We were able to detect schistosomiasis at the third week post-infection, which was one week earlier than "gold standard" methods. We found that S. japonicum infection caused significant elevation of urinary 3-ureidopropionate, a uracil catabolic product, and disturbance of lipid metabolism, stimulation of glycolysis, depression of tricarboxylic acid cycle and disruption of gut microbiota regulations. We further found that the changes of 3-ureidopropionate and overall metabolic changes in both urinary and plasma samples were closely correlated with the time-course of disease progression. Furthermore, such changes together with liver tissue metabonome were clearly associated with the worm-burdens. These findings provided more insightful understandings of host biological responses to the infection and demonstrated that metabonomic analysis is potentially useful for early detection of schistosomiasis and comprehension of the mechanistic aspects of disease progression.
authors.affiliation: State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Center for Magnetic Resonance, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan, People's Republic of China.
authors.forename: Junfang
authors.initials: J
authors.lastname: Wu
authors.forename: Wenxin
authors.initials: W
authors.lastname: Xu
authors.forename: Zhenping
authors.initials: Z
authors.lastname: Ming
authors.forename: Huifen
authors.initials: H
authors.lastname: Dong
authors.forename: Huiru
authors.initials: H
authors.lastname: Tang
authors.forename: Yulan
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D001826:Body Fluids / Q000737:chemistry*; D002952:Citric Acid Cycle; D005260:Female; D006019:Glycolysis; D050356:Lipid Metabolism; D008099:Liver / Q000737:chemistry* / Q000473:pathology; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008807:Mice, Inbred BALB C; D012547:Schistosoma / Q000472:pathogenicity*; D012552:Schistosomiasis / Q000503:physiopathology*; D014508:Urea / Q000031:analogs & derivatives / Q000652:urine
chemical_list: D014508:Urea
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