title: Glycosylation and production characteristics of epothilones in alkali-tolerant Sorangium cellulosum strain So0157-2.
abstract: 3-O-alpha-D-ribofuranosyl epothilone A (epothiloneoside A) is a major component of glycosylated epothilones in Sorangium cellulosum strain So0157-2. The production and glycosylation ratios of epothiloneoside A in both solid and liquid culture conditions with various pH values and carbon sources were studied. The results showed that glycosylation occurs whenever epothilones are produced, regardless of changes in pH values, production time curves, and different carbon sources. We suggest that glycosylation is a stable process, paralleling the biosynthesis of epothilones in the So0157-2 strain.
authors.affiliation: State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, Jinan, P. R. China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhao
authors.forename: Peng-fei
authors.initials: PF
authors.lastname: Li
authors.forename: Chun-hua
authors.initials: CH
authors.lastname: Lu
authors.forename: Shu-guang
authors.initials: SG
authors.lastname: Li
authors.forename: Yue-mao
authors.initials: YM
authors.lastname: Shen
authors.forename: Yue-zhong
authors.initials: YZ
authors.lastname: Li
mesh_terms: D000468:Alkalies / Q000378:metabolism*; D045744:Cell Line, Tumor; D034261:Epothilones / Q000378:metabolism* / Q000494:pharmacology; D006031:Glycosylation; D006801:Humans; D008969:Molecular Sequence Data; D009231:Myxococcales / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D010802:Phylogeny; D012988:Soil Microbiology
chemical_list: D000468:Alkalies; D034261:Epothilones
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