Integration pattern of HIV-1 based lentiviral vector carrying recombinant coagulation factor VIII in Sk-Hep and 293T cells.
293T and Sk-Hep-1 cells were transduced with a replication-defective self-inactivating HIV-1 derived vector carrying FVIII cDNA. The genomic DNA was sequenced to reveal LTR/human genome junctions and integration sites. One hundred and thirty-two sequences matched human sequences, with an identity of at least 98%. The integration sites in 293T-FVIIIDB and in Sk-Hep-FVIIIDB cells were preferentially located in gene regions. The integrations in both cell lines were distant from the CpG islands and from the transcription start sites. A comparison between the two cell lines showed that the lentiviral-transduced DNA had the same preferred regions in the two different cell lines.
Russo-Carbolante
Elisa Maria de Sousa
EM
Department of Clinical, Toxicological and Food Science Analysis, Faculty of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil. elisa@fcfrp.usp.br
Picanço-Castro
Virgínia
V
Alves
Daiani Cristina Cilão
DC
Fernandes
Andrielle Castilho
AC
Almeida-Porada
Graça
G
Tonn
Torsten
T
Covas
Dimas Tadeu
DT
D002460:Cell Line; D005169:Factor VIII / Q000096:biosynthesis* / Q000235:genetics*; D005822:Genetic Vectors; D015497:HIV-1 / Q000235:genetics / Q000502:physiology*; D022781:Hepatocytes / Q000821:virology; D006801:Humans; D007668:Kidney / Q000821:virology; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D017422:Sequence Analysis, DNA; D016662:Virus Integration
D011994:Recombinant Proteins; C078147:F8 protein, human; D005169:Factor VIII