title: Metabonomic study on women of reproductive age treated with nutritional intervention: screening potential biomarkers related to neural tube defects occurrence.
abstract: Nutritional intervention is effective in reducing the risk of neural tube defects (NTDs). To determine the effects of nutritional supplementation on human metabolism, a metabonomic study was carried out on 96 women of reproductive age. Subjects with nutritional intervention were given fortified wheat flour (containing folic acid, vitamin B₁, vitamin B₂, ferric sodium edetate and zinc oxide) for 8 months. Serum metabolic fingerprinting was detected via ultraperformance liquid chromatography in tandem with time-of-flight mass spectrometry (UPLC-TOF MS), and data acquired was processed by multivariate statistical analysis. The result revealed a significant difference between the control and intervention group. Twenty potential biomarkers, including fructose 6-phosphate, sphingosine 1-phosphate, docosahexaenoic acid and hexadecanoic acid, were located and identified by the accurate mass measurement of TOF MS. These compounds are believed to be functionally related to anti-oxidative competence in vivo. In conclusion, metabonomics study is a valuable approach in exploring the effect mechanism of nutritional intervention on NTD prevention.
authors.affiliation: School of Pharmacy, East-China University of Science and Technology, Shanghai 200237, People's Republic of China.
authors.forename: Zhiting
authors.initials: Z
authors.lastname: Jiang
authors.forename: Qionglin
authors.initials: Q
authors.lastname: Liang
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Zheng
authors.forename: Lijun
authors.initials: L
authors.lastname: Pei
authors.forename: Ting
authors.initials: T
authors.lastname: Zhang
authors.forename: Yiming
authors.initials: Y
authors.lastname: Wang
authors.forename: Guoan
authors.initials: G
authors.lastname: Luo
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood; D019587:Dietary Supplements; D005227:Fatty Acids / Q000097:blood; D005260:Female; D005433:Flour; D005527:Food, Fortified; D005636:Fructosephosphates / Q000097:blood; D006801:Humans; D016018:Least-Squares Analysis; D008246:Lysophospholipids / Q000097:blood; D055442:Metabolome; D055432:Metabolomics / Q000379:methods*; D015999:Multivariate Analysis; D009436:Neural Tube Defects / Q000378:metabolism* / Q000517:prevention & control; D015203:Reproducibility of Results; D013110:Sphingosine / Q000031:analogs & derivatives / Q000097:blood
chemical_list: D015415:Biomarkers; D005227:Fatty Acids; D005636:Fructosephosphates; D008246:Lysophospholipids; C060506:sphingosine 1-phosphate; C027618:fructose-6-phosphate; D013110:Sphingosine