title: A role for pectin de-methylesterification in a developmentally regulated growth acceleration in dark-grown Arabidopsis hypocotyls.
abstract: • We focused on a developmentally regulated growth acceleration in the dark-grown Arabidopsis hypocotyl to study the role of changes in cell wall metabolism in the control of cell elongation. • To this end, precise transcriptome analysis on dissected dark-grown hypocotyls, Fourier transform infrared (FT-IR) microspectroscopy and kinematic analysis were used. • Using a cellulose synthesis inhibitor, we showed that the growth acceleration marks a developmental transition during which growth becomes uncoupled from cellulose synthesis. We next investigated the cellular changes that take place during this transition. FT-IR microspectroscopy revealed significant changes in cell wall composition during, but not after, the growth acceleration. Transcriptome analysis suggested a role for cell wall remodeling, in particular pectin modification, in this growth acceleration. This was confirmed by the overexpression of a pectin methylesterase inhibitor, which caused a delay in the growth acceleration. • This study shows that the acceleration of cell elongation marks a developmental transition in dark-grown hypocotyl cells and supports a role for pectin de-methylesterification in the timing of this event.
authors.affiliation: Institut Jean-Pierre Bourgin, UMR1318 INRA-AgroParisTech, INRA Centre de Versailles-Grignon, Versailles, France.
authors.forename: Sandra
authors.initials: S
authors.lastname: Pelletier
authors.forename: Jürgen
authors.initials: J
authors.lastname: Van Orden
authors.forename: Sebastian
authors.initials: S
authors.lastname: Wolf
authors.forename: Kris
authors.initials: K
authors.lastname: Vissenberg
authors.forename: Julien
authors.initials: J
authors.lastname: Delacourt
authors.forename: Yves Assoumou
authors.initials: YA
authors.lastname: Ndong
authors.forename: Jérôme
authors.initials: J
authors.lastname: Pelloux
authors.forename: Volker
authors.initials: V
authors.lastname: Bischoff
authors.forename: Aurélie
authors.initials: A
authors.lastname: Urbain
authors.forename: Grégory
authors.initials: G
authors.lastname: Mouille
authors.forename: Gaëtan
authors.initials: G
authors.lastname: Lemonnier
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Renou
authors.forename: Herman
authors.initials: H
authors.lastname: Höfte
mesh_terms: D017360:Arabidopsis / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism; D002265:Carboxylic Ester Hydrolases / Q000037:antagonists & inhibitors*; D002473:Cell Wall / Q000378:metabolism*; D002482:Cellulose / Q000096:biosynthesis; D003624:Darkness; D004951:Esterification; D015870:Gene Expression; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D018546:Hypocotyl / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D010368:Pectins / Q000378:metabolism*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D010368:Pectins; D002482:Cellulose; D002265:Carboxylic Ester Hydrolases; C022323:pectinesterase