[Protective effects of asiaticoside on sepsis-induced acute kidney injury in mice].
OBJECTIVE
The present study is to investigate the protective effects of asiaticoside on sepsis-induced acute kidney injury in mice.


METHODS
With the sepsis induced by cecal ligation and puncture (CLP), forty eight kunming mice were randomly divided into four groups as sham operated, CLP treated, CLP + asiaticoside 15, 45 mg x kg(-1) groups. General conditions and the amount of dead rate of mice were observed. The BUN and Cr levels were observed by the kits. IL-6 in serum was assayed by enzyme-linked immunosorbent assay (ELISA). Kidney tissues were harvested for determination of iNOS expression by Western blotting analysis. The pathologic changes were observed under electron microscope via hematoxylin-eosin (HE) stain.


RESULTS
Compared with CLP group, the death rate, the levels of BUN, Cr, IL-6, and iNOS protein expression of asiaticoside groups were significantly reduced. The pathologic changes in kidney tissues induced by sepsis were significantly attenuated dose-dependently by asiaticoside under electron microscope.


CONCLUSIONS
Asiaticoside has protective effects against sepsis-induced acute kidney injury, which were probably associated with the inhibition of IL-6 in serum and iNOS protein in kidney tissues.
Zheng
Jiajia
J
Chongqing Key of Biochemistry and Molecular Pharmacology, Chongqing Medical University, Chongqing 400016, China.
Zhang
Li'na
L
Wu
Mengjiao
M
Li
Xiaohui
X
Zhang
Li
L
Wan
Jingyuan
J
D058186:Acute Kidney Injury / Q000188:drug therapy / Q000209:etiology / Q000517:prevention & control*; D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D010936:Plant Extracts / Q000008:administration & dosage*; D011897:Random Allocation; D018805:Sepsis / Q000150:complications*; D014315:Triterpenes / Q000008:administration & dosage*
D010936:Plant Extracts; D014315:Triterpenes; C004446:asiaticoside