A nanoparticle dispersion method for in vitro and in vivo nanotoxicity study.
The dispersion in air of nanoparticles of different sizes, materials and morphologies with controlled agglomeration involving aerosol delivery for in vivo and in vitro studies is one of the most difficult challenges in the field of nanoparticle toxicology. We describe here a nanoparticle dispersion system using an electrospray method to deliver airborne nanoparticles (approximately 10-100 nm) with spatial uniformity and controllable particle concentration for in vitro and in vivo studies. With the dispersion method, single nanoparticles (polystyrene latex particles, TiO(2), Au, Mn, quantum dots, and carbon nanotubes) can be delivered to cells and animals via the air. The degree of agglomeration can be controlled by changing the suspension feeding rate to simulate realistic conditions for exposure studies.
Kim
Seong C
SC
Department of Mechanical Engineering, University of Minnesota, 111 Church Street SE, Minneapolis, MN 55455, USA.
Chen
Da-Ren
DR
Qi
Chaolong
C
Gelein
Robert M
RM
Finkelstein
Jacob N
JN
Elder
Alison
A
Bentley
Karen
K
Oberdörster
Günter
G
Pui
David Y H
DY
D000336:Aerosols / Q000737:chemistry / Q000633:toxicity*; D000818:Animals; D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods; D006801:Humans; D019570:Inhalation Exposure; D053758:Nanoparticles / Q000633:toxicity*; D037742:Nanotubes, Carbon / Q000633:toxicity*; D010316:Particle Size; D051381:Rats
D000336:Aerosols; D037742:Nanotubes, Carbon