title: HPLC-MS analysis of ethanol extract of Corydalis yanhusuo and simultaneous determination of eight protoberberine quaternary alkaloids by HPLC-DAD.
abstract: An accurate and simple analysis method by high-performance liquid chromatography (HPLC)-electrospray tandem mass spectrometry (ESI-MS-MS) for the ethanol extract of Corydalis yanhusuo was established in this study. In this method, a C(18) column was used, and the mobile phase was water (0.2% acetic acid, 0.1% triethylamine, v/v)-acetonitrile (24:76, v/v). Under the optimal analysis conditions established in this study, eight protoberberine quaternary alkaloids in the ethanol extract of Corydalis yanhusuo were well separated and identified, respectively. Eight regression equations showed a good linear relationship between the peak area of each marker and concentration (r = 0.9990-0.9995). The various other aspects of analysis (i.e., peak purity, precision, recovery, and repeatability) were also validated. For the eight components, the recoveries were found to be 98.9%, 99.2%, 105.0%, 97.7%, 101.4%, 98.6%, 98.5%, and 98.7%, respectively. The limits of detection were 14.6, 42.5, 22.4, 17.9, 25.4, 10.0, 18.7, 3.9 ng/mL, respectively, and the limits of quantitation were 48.6, 141.7, 74.6, 59.6, 84.8, 33.2, 62.3, and 12.9 ng/mL, respectively. The developed method is useful for the simultaneous determination of these alkaloids in a large number of ethanol extracts of Corydalis yanhusuo.
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100094, China.
authors.forename: Xing-Ye
authors.initials: XY
authors.lastname: Cheng
authors.forename: Yue
authors.initials: Y
authors.lastname: Shi
authors.forename: Shun-Liang
authors.initials: SL
authors.lastname: Zhen
authors.forename: Hong
authors.initials: H
authors.lastname: Sun
authors.forename: Wen
authors.initials: W
authors.lastname: Jin
mesh_terms: D000470:Alkaloids / Q000032:analysis* / Q000302:isolation & purification; D001600:Berberine Alkaloids / Q000032:analysis* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D031311:Corydalis / Q000737:chemistry*; D000431:Ethanol; D016014:Linear Models; D010936:Plant Extracts / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000470:Alkaloids; D001600:Berberine Alkaloids; D010936:Plant Extracts; C009090:protoberberine; D000431:Ethanol