In vivo cellular MRI of dendritic cell migration using micrometer-sized iron oxide (MPIO) particles.
OBJECTIVE
This study seeks to assess the use of labeling with micron-sized iron oxide (MPIO) particles for the detection and quantification of the migration of dendritic cells (DCs) using cellular magnetic resonance imaging (MRI).


METHODS
DCs were labeled with red fluorescent MPIO particles for detection by cellular MRI and a green fluorescent membrane dye (PKH67) for histological detection. MPIO-labeled DCs or unlabeled control DCs were injected into mice footpads at two doses (0.1 × 10(6) and 1 × 10(6)). Images were acquired at 3 Tesla before DC injection and 2, 3, and 7 days post-DC injection.


RESULTS
Labeling DCs with MPIO particles did not affect viability, but it did alter markers of DC activation and maturation. MRI and fluorescence microscopy allowed for the detection of MPIO-labeled DCs within the draining popliteal nodes after their injection into the footpad.


CONCLUSIONS
This paper presents the first report of the successful use of fluorescent MPIO particles to label and track DC migration.
Rohani
Roja
R
Department of Medical Biophysics, University of Western Ontario, London, Ontario, Canada.
de Chickera
Sonali N
SN
Willert
Christy
C
Chen
Yuhua
Y
Dekaban
Gregory A
GA
Foster
Paula J
PJ
D000818:Animals; D017209:Apoptosis; D001854:Bone Marrow Cells / Q000166:cytology; D002465:Cell Movement; D002470:Cell Survival; D003713:Dendritic Cells / Q000166:cytology* / Q000378:metabolism; D005290:Ferric Compounds / Q000737:chemistry* / Q000378:metabolism*; D005453:Fluorescence; D008198:Lymph Nodes / Q000473:pathology; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009930:Organic Chemicals / Q000378:metabolism; D010316:Particle Size; D010641:Phenotype; D013194:Staining and Labeling
D005290:Ferric Compounds; D009930:Organic Chemicals; C451241:PKH67; C000499:ferric oxide
Proc Natl Acad Sci U S A. 2004 Jul 27;101(30):10901-6
15256592
Clin Cancer Res. 2003 Nov 1;9(14):5091-100
14613986
Cancer Res. 2003 Jan 1;63(1):12-7
12517769
Magn Reson Med. 2006 Nov;56(5):1001-10
17029229
Contrast Media Mol Imaging. 2007 May-Jun;2(3):147-53
17541955
Proc Natl Acad Sci U S A. 2001 Jul 17;98(15):8750-5
11447263
J Neuroimmunol. 2004 Nov;156(1-2):42-57
15465595
Magn Reson Med. 2003 May;49(5):968-71
12704781
J Magn Reson Imaging. 2007 Jun;25(6):1210-8
17520727
Magn Reson Med. 2005 Feb;53(2):312-20
15678551
Anal Chem. 1989 Sep 1;61(17):1851-7
2802147
Mol Ther. 2008 Dec;16(12):2022-9
18797450
Cancer Immunol Immunother. 2008 Oct;57(10):1559-68
18618110
J Neurotrauma. 2009 Sep;26(9):1509-19
19663686
Trends Immunol. 2001 Dec;22(12):691-700
11739000
Nature. 1998 Mar 19;392(6673):245-52
9521319
J Neurosurg. 2007 Feb;106(2):306-13
17410716
Nature. 2004 Jan 8;427(6970):154-9
14712275
Cancer Invest. 2003;21(6):873-86
14735692
Cancer Immunol Immunother. 2008 Jan;57(1):63-71
17609951
Nat Biotechnol. 2005 Nov;23(11):1407-13
16258544
J Exp Med. 2003 Aug 18;198(4):615-21
12925677
Nat Immunol. 2004 Jun;5(6):583-9
15133508
Eur J Immunol. 2006 Sep;36(9):2544-55
16909432
Magn Reson Med. 2005 Feb;53(2):329-38
15678543
J Exp Med. 1999 Dec 6;190(11):1669-78
10587357
Clin Cancer Res. 1999 Jun;5(6):1289-97
10389911
J Neurotrauma. 2009 May;26(5):753-62
19397403
Blood. 2003 Aug 1;102(3):867-72
12676779
Magn Reson Med. 2003 Jun;49(6):1006-13
12768577
J Immunother. 2009 Apr;32(3):240-51
19242376
Cancer Res. 2009 Apr 1;69(7):3180-7
19276358
Circulation. 2008 Jul 8;118(2):149-56
18591438
Skin Pharmacol Physiol. 2006;19(3):124-31
16612139
Nature. 2007 Sep 27;449(7161):419-26
17898760
Int J Cancer. 2007 Mar 1;120(5):978-84
17163419
Magn Reson Med. 2006 Jan;55(1):23-9
16342157