Enhancement of magnetotactic bacterial yield in a modified MSGM medium without alteration of magnetosomes properties.
Magnetotactic bacteria (MTB), Magnetospirillum magnetotacticum (MS-1) were successfully grown in modified magnetic spirillum growth medium (MSGM) at normal laboratory environment. About five-time increase in the bacterial yield was achieved in the modified MSGM medium without compromising their magnetosomes properties. Transmission electron and scanning electron microscopy (TEM & SEM) were used for morphological study of MTB. Energy dispersive analysis of X-rays (EDAX) and vibrating sample magnetometer (VSM) techniques, respectively, were used to elucidate the phase and magnetization in the bacterially synthesized magnetosomes. These studies were important to cross-check the morphology of magnetosomes, as the formation of magnetosomes was highly sensitive to environmental conditions.
Kundu
Srikanya
S
School of Basic Medical Sciences, Department of Physics, University of Pune, Pune 411 007, India. srikanya19@gmail.com
Kulkarni
Gauri R
GR
D001431:Bacteriological Techniques; D003470:Culture Media / Q000494:pharmacology*; D004577:Electron Probe Microanalysis; D052203:Ferrosoferric Oxide / Q000032:analysis; D008280:Magnetics; D055944:Magnetosomes / Q000502:physiology* / Q000648:ultrastructure; D042201:Magnetospirillum / Q000187:drug effects* / Q000502:physiology / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning
D003470:Culture Media; D052203:Ferrosoferric Oxide