title: Imaging mass spectrometry of glycolipids.
abstract: Mass spectrometry (MS) is an analytical technique that separates ionized molecules using differences in their mass, and can be used to determine the structure of the molecules. Matrix-assisted laser desorption/ionization (MALDI) is one of the most commonly used ionization methods for this procedure. A new technical method, imaging mass spectrometry (IMS), which is a two-dimensional MS, enables molecular imaging of tissue sections by the use of the MALDI-MS method. In this chapter, we briefly discuss available methods for analyzing glycolipids by IMS. We describe sample detection strategies, and also introduce a representative example of its research application.
authors.affiliation: Department of Molecular Anatomy, Hamamatsu University School of Medicine, Handayama, Higashi-ku, Hamamatsu, Shizuoka, Japan.
authors.forename: Naoko
authors.initials: N
authors.lastname: Goto-Inoue
authors.forename: Takahiro
authors.initials: T
authors.lastname: Hayasaka
authors.forename: Mitsutoshi
authors.initials: M
authors.lastname: Setou
mesh_terms: D000818:Animals; D006017:Glycolipids / Q000737:chemistry*; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D051379:Mice; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D006017:Glycolipids; C009233:seminolipid