Quick assessment of cytotoxins effect on Daphnia magna using in vivo fluorescence microscopy.
A novel approach to contaminant toxicity screening is proposed. The use of fluorescent microscopy with fluorescent dyes allows for assessing intoxication of Daphnia magna tissues, at various stages of exposure, to contaminants present in water. As shown, D. magna may not only be used as a test species in toxicity tests based on its lethality, but due to its translucency and application of fluorescent probes, separate steps of its intoxication and dying can be visualized. Using a variety of fluorescent probes, the present study also contributes to a better understanding of the toxicity mechanisms.
Teplova
Vera V
VV
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Moscow Region, Pushchino, 142290 Russia.
Andreeva-Kovalevskaya
Zhanna I
ZI
Sineva
Elena V
EV
Solonin
Alexander S
AS
D000818:Animals; D016923:Cell Death / Q000187:drug effects; D003621:Daphnia / Q000166:cytology / Q000187:drug effects*; D005456:Fluorescent Dyes / Q000737:chemistry / Q000633:toxicity*; D007928:Lethal Dose 50; D008856:Microscopy, Fluorescence; D013329:Structure-Activity Relationship; D013997:Time Factors; D014874:Water Pollutants, Chemical / Q000737:chemistry / Q000633:toxicity*
D005456:Fluorescent Dyes; D014874:Water Pollutants, Chemical