title: Selection and characterization of a multivalent Salmonella phage and its production in a nonpathogenic Escherichia coli strain.
abstract: We report the selection and amplification of the broad-host-range Salmonella phage phi PVP-SE1 in an alternative nonpathogenic host. The lytic spectrum and the phage DNA restriction profile were not modified upon replication in Escherichia coli Bl21, suggesting the possibility of producing this phage in a nonpathogenic host, contributing to the safety and easier approval of a product based on this Salmonella biocontrol agent.
authors.affiliation: Institute for Biotechnology and Bioengineering, Center of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4700-057 Braga, Portugal.
authors.forename: S B
authors.initials: SB
authors.lastname: Santos
authors.forename: E
authors.initials: E
authors.lastname: Fernandes
authors.forename: C M
authors.initials: CM
authors.lastname: Carvalho
authors.forename: S
authors.initials: S
authors.lastname: Sillankorva
authors.forename: V N
authors.initials: VN
authors.lastname: Krylov
authors.forename: E A
authors.initials: EA
authors.lastname: Pleteneva
authors.forename: O V
authors.initials: OV
authors.lastname: Shaburova
authors.forename: A
authors.initials: A
authors.lastname: Nicolau
authors.forename: E C
authors.initials: EC
authors.lastname: Ferreira
authors.forename: J
authors.initials: J
authors.lastname: Azeredo
mesh_terms: D000818:Animals; D004926:Escherichia coli / Q000821:virology*; D006801:Humans; D046529:Microscopy, Electron, Transmission; D010641:Phenotype; D012150:Polymorphism, Restriction Fragment Length / Q000235:genetics; D012480:Salmonella Infections / Q000517:prevention & control; D012481:Salmonella Infections, Animal / Q000517:prevention & control; D012483:Salmonella Phages / Q000235:genetics / Q000254:growth & development / Q000472:pathogenicity* / Q000502:physiology / Q000648:ultrastructure; D019779:Salmonella enterica / Q000821:virology
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