title: Surface-modified microprojection arrays for intradermal biomarker capture, with low non-specific protein binding.
abstract: Minimally invasive biosensors are of great interest for rapid detection of disease biomarkers for diagnostic screening at the point-of-care. Here we introduce a device which extracts disease-specific biomarkers directly from the upper dermis, without the needle and syringe or resource-intensive blood processing. Using antigen-specific antibodies raised in mice as a model system, we confirm the analytical specificity and sensitivity of the antibody capture and extraction in comparison to the conventional methods based on needle/syringe blood draw followed by processing and antigen-specific ELISAs.
authors.affiliation: The University of Queensland, Australian Institute for Bioengineering and Nanotechnology, St Lucia, QLD 4072, Australia. s.corrie1@uq.edu.au
authors.forename: Simon R
authors.initials: SR
authors.lastname: Corrie
authors.forename: Germain J P
authors.initials: GJ
authors.lastname: Fernando
authors.forename: Michael L
authors.initials: ML
authors.lastname: Crichton
authors.forename: Marion E G
authors.initials: ME
authors.lastname: Brunck
authors.forename: Chris D
authors.initials: CD
authors.lastname: Anderson
authors.forename: Mark A F
authors.initials: MA
authors.lastname: Kendall
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis*; D015374:Biosensing Techniques / Q000295:instrumentation*; D001800:Blood Specimen Collection / Q000295:instrumentation*; D002197:Capillary Action; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007118:Immunoassay / Q000295:instrumentation*; D051379:Mice; D008810:Mice, Inbred C57BL; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D040081:Protein Array Analysis / Q000295:instrumentation*; D012867:Skin / Q000378:metabolism*
chemical_list: D015415:Biomarkers