title: Up-regulation of PSF1 promotes the growth of breast cancer cells.
abstract: PSF1 is a subunit of the GINS complex that functions along with the MCM2-7 complex and Cdc45 in eukaryotic DNA replication. Although mammalian PSF1 is predominantly expressed in highly proliferating cells and organs, little is known about the roles of PSF1 in mature cells or cancer cells. We found that PSF1 was expressed at relatively high levels in breast tumor cells, but at low levels in normal breast cells. Knockdown of PSF1 expression using small interfering RNA (siRNA) slowed the growth of breast cancer cell lines by delaying DNA replication but did not affect proliferation of normal human mammary epithelial cells. Reduced PSF1 expression also inhibited anchorage-independent growth in breast cancer cell lines. These results suggest that PSF1 over-expression is specifically involved in breast cancer cell growth. Therefore, PSF1 inhibition might provide new therapeutic approaches for breast cancer.
authors.affiliation: Center for Medical Genomics, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045, Japan.
authors.forename: Izumi
authors.initials: I
authors.lastname: Nakahara
authors.forename: Mamiko
authors.initials: M
authors.lastname: Miyamoto
authors.forename: Tatsuhiro
authors.initials: T
authors.lastname: Shibata
authors.forename: Sadako
authors.initials: S
authors.lastname: Akashi-Tanaka
authors.forename: Takayuki
authors.initials: T
authors.lastname: Kinoshita
authors.forename: Kaoru
authors.initials: K
authors.lastname: Mogushi
authors.forename: Kohtaro
authors.initials: K
authors.lastname: Oda
authors.forename: Masaya
authors.initials: M
authors.lastname: Ueno
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Takakura
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Mizushima
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Tanaka
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Ohta
mesh_terms: D001943:Breast Neoplasms / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D004847:Epithelial Cells / Q000378:metabolism; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D042361:Mammary Glands, Human / Q000166:cytology / Q000378:metabolism / Q000473:pathology; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D004268:DNA-Binding Proteins; C547590:GINS1 protein, human; D034741:RNA, Small Interfering