A doubly signal-amplified DNA detection method based on pre-complexed [Ru(bpy)3]2+-doped silica nanoparticles.
Easy detection: The target DNA in a 10-100 aM range can be detected by pre-complexed nanoparticles without additional amplification or target labeling. The [Ru(bpy)(3)](2+)-doped silica nanoparticles are hybridized to form a complex with highly enhanced sensitivity (see scheme). This method will be a significant improvement over conventional microarray/fluorescence readout systems.
Bae
Se Won
SW
Department of Chemistry, Seoul National University, Seoul 151-747, Korea.
Cho
Min Sun
MS
Hur
Seong-Suk
SS
Chae
Chi-Bom
CB
Chung
Doo Soo
DS
Yeo
Woon-Seok
WS
Hong
Jong-In
JI
D004247:DNA / Q000032:analysis*; D015342:DNA Probes / Q000737:chemistry; D061925:Immobilized Nucleic Acids / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry*; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D009942:Organometallic Compounds / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*
D015342:DNA Probes; D061925:Immobilized Nucleic Acids; D009942:Organometallic Compounds; C415768:tris(2,2'-bipyridyl)ruthenium(II); D012822:Silicon Dioxide; D004247:DNA