Further diversity of the Staphylococcus intermedius group and heterogeneity in the MboI restriction site used for Staphylococcus pseudintermedius species identification.
Species identification of 200 beta-hemolysin-producing canine staphylococcal isolates was performed using a recently described polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method (based on MboI restriction of a pta gene fragment), supplemented with biochemical testing and sequencing of housekeeping genes. The PCR-RFLP method misclassified a small fraction (approximately 1%) of the Staphylococcus pseudintermedius population as a result of heterogeneity in the MboI restriction site. A potentially novel species within the Staphylococcus intermedius group (SIG) was found, having closest similarity to S. intermedius based on sequence comparison to the genes sodA, pta, hsp60, tuf, and full-length 16S ribosomal DNA, thus demonstrating further species diversity within the SIG.
Slettemeås
Jannice Schau
JS
Section of Bacteriology, National Veterinary Institute, Oslo, Norway. jannice.schau-slettemeas@vetinst.no
Mikalsen
Jarle
J
Sunde
Marianne
M
D000818:Animals; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D004283:Dog Diseases / Q000382:microbiology; D004285:Dogs; D006801:Humans; D016106:Methicillin Resistance; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D015183:Restriction Mapping / Q000379:methods; D016415:Sequence Alignment; D013203:Staphylococcal Infections / Q000453:epidemiology / Q000635:transmission / Q000662:veterinary; D013210:Staphylococcus / Q000145:classification / Q000235:genetics*; D013211:Staphylococcus aureus / Q000235:genetics
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S