The impact of zero-valent iron nanoparticles on a river water bacterial community.
Zero-valent iron (ZVI) nanoparticles are of interest because of their many potential biomedical and environmental applications. However, these particles have recently been reported to be cytotoxic to bacterial cells. The overall objective of this study was to determine the impact of 100mg/L ZVI nanoparticles on the diversity and structure of an indigenous river water bacterial community. Response during exposure for 36 days was determined by denaturing gel gradient electrophoresis (DGGE) analysis of bacterial 16S rRNA genes, amplified from extracted DNA, and viable and total cell abundances were determined by plate counting and fluorescent microscopy of DAPI-stained cells. Changes in river water chemistry were also monitored. Addition of ZVI nanoparticles led to a rapid decrease in oxidation-reduction potential (ORP) (+196 to -281 mV) and dissolved oxygen (DO) concentration (8.2-0.6 mg/L), both of which stabilized during the experiment. Interestingly, both viable and total bacterial cell abundances increased and pH decreased, characteristic of an active microbial community. Total community structure was visualized using rank-abundance plots fitted with linear regression models. The slopes of the regression models were used as a descriptive statistic of changes in evenness over time. Importantly, despite bacterial growth, addition of ZVI nanoparticles did not influence bacterial community structure.
Barnes
Robert J
RJ
Department of Engineering Science, University of Oxford, Parks Road, OX1 3PJ, UK.
van der Gast
Christopher J
CJ
Centre for Ecology and Hydrology, Mansfield Road, Oxford, OX1 3SR, UK.
Riba
Olga
O
Interface Analysis Centre, University of Bristol, 121 St. Michael's Hill, Bristol BS2 8BS, UK.
Lehtovirta
Laura E
LE
Institute of Biological and Environmental Sciences, University of Aberdeen, St. Machar Drive, AB24 3UU, UK.
Prosser
James I
JI
Institute of Biological and Environmental Sciences, University of Aberdeen, St. Machar Drive, AB24 3UU, UK.
Dobson
Peter J
PJ
Begbroke Directorate, Oxford University Begbroke Science Park, Sandy Lane, OX5 1PF, UK.
Thompson
Ian P
IP
Department of Engineering Science, University of Oxford, Parks Road, OX1 3PJ, UK. Electronic address: Ian.Thompson@eng.ox.ac.uk.
D001419:Bacteria / Q000302:isolation & purification*; D001483:Base Sequence; D015169:Colony Count, Microbial; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D005618:Fresh Water / Q000382:microbiology*; D007501:Iron / Q000737:chemistry*; D053768:Metal Nanoparticles; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence
D017931:DNA Primers; D007501:Iron