[Screening of three straw-cellulose degrading microorganism].
OBJECTIVE
The aim of this study was to screen straw-cellulose degrading microorganisms and to investigate their degradation ability of straw-cellulose.


METHODS
The methods used to screen the high effect straw-cellulose degrading microorganism included the traditional isolation methods of straw-cellulose degrading microorganism such as holes observation method on filter paper sheet, disintegration test of filter paper scrip, hydrolysis spot diameter measurement method of CMC-Na, weight lose assay method of straw, measurement method of cellulose decomposition rate, measurement method of extracellular enzyme activity.


RESULTS
We isolated 3 fungi with cellulose degrading ability, of which 98MJ was identified as Penicillium oxalicum, W3 as Trichoderma sp., and W4 as Penicillium expansum. Strain W4 possessed high straw-cellulose degrading ability with straw-cellulose degrading rate of 56.3%, cellulose 59.06%, hemicellulose 78.75% and lignin 33.79% in 10 days.


CONCLUSIONS
Strain W4 was a cellulase-producing strain with broad development potential.
Wang
Hongyuan
H
Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China. wanghy@caas.ac.cn
Fan
Bingquan
B
D001673:Biodegradation, Environmental; D002480:Cellulase / Q000378:metabolism; D002482:Cellulose / Q000378:metabolism*; D004783:Environmental Microbiology; D005656:Fungal Proteins / Q000378:metabolism; D005658:Fungi / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D008969:Molecular Sequence Data; D010407:Penicillium / Q000145:classification / Q000201:enzymology / Q000302:isolation & purification / Q000378:metabolism*; D010802:Phylogeny; D014242:Trichoderma / Q000145:classification / Q000201:enzymology / Q000302:isolation & purification / Q000378:metabolism*
D005656:Fungal Proteins; D002482:Cellulose; D002480:Cellulase