Multivalent ligands for siglecs.
Siglecs have emerged as an important family of immunomodulatory glycan-binding proteins that can bind sialoside ligands both on the same cell surface, in cis, and on other cells, in trans. Expression of siglecs varies among a variety of immune cells, and tools to probe siglecs on these cells are crucial to understanding their function. In designing synthetic ligands, competition by cis ligands requires the use of multivalency to achieve sufficient avidity to stably bind siglecs on native cells. This chapter describes the use of multivalent ligands to probe cell surfaces, as well as to investigate ligand binding to recombinant siglecs.
O'Reilly
Mary K
MK
Department of Chemical Physiology, The Scripps Research Institute, La Jolla, California, USA.
Paulson
James C
JC
D000818:Animals; D005434:Flow Cytometry; D006801:Humans; D037102:Lectins / Q000235:genetics / Q000378:metabolism*; D008024:Ligands; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000378:metabolism; D018448:Models, Immunological; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D063265:Sialic Acid Binding Immunoglobulin-like Lectins
D037102:Lectins; D008024:Ligands; D008562:Membrane Glycoproteins; D011994:Recombinant Proteins; D063265:Sialic Acid Binding Immunoglobulin-like Lectins
Eur J Immunol. 2002 Jul;32(7):1924-32
12115612
Microbiology (Reading). 2007 Sep;153(Pt 9):2817-2822
17768226
Trends Pharmacol Sci. 2009 May;30(5):240-8
19359050
Mol Cell Biol. 2007 Aug;27(16):5699-710
17562860
Biol Chem. 2001 Feb;382(2):291-7
11308027
J Exp Med. 2010 Jan 18;207(1):173-87
20038598
Nat Chem Biol. 2005 Jul;1(2):93-7
16408005
Carbohydr Res. 2002 Oct 8;337(18):1605-13
12423961
J Biol Chem. 1996 Apr 19;271(16):9267-72
8621587
J Biol Chem. 1995 Nov 3;270(44):26184-91
7592823
J Pharmacol Exp Ther. 2009 Aug;330(2):608-12
19458105
Biochem Soc Trans. 2008 Dec;36(Pt 6):1467-71
19021577
Blood. 2010 Jun 10;115(23):4778-86
20181615
Ann N Y Acad Sci. 2008 Nov;1143:61-82
19076345
Infect Immun. 2006 Jul;74(7):4133-41
16790787
Mol Microbiol. 2003 Sep;49(5):1213-25
12940982
FEBS Lett. 2010 Feb 5;584(3):555-61
19945458
Mol Divers. 2006 Nov;10(4):555-66
16972014
Proc Natl Acad Sci U S A. 1998 Jun 23;95(13):7469-74
9636173
J Immunol. 2006 Sep 1;177(5):2994-3003
16920935
J Biol Chem. 2005 Feb 11;280(6):4307-12
15563466
PLoS One. 2008 Apr 16;3(4):e1967
18414664
J Exp Med. 2002 May 6;195(9):1207-13
11994426
J Cell Biol. 1989 Feb;108(2):389-400
2563728
Methods Mol Biol. 2010;600:67-78
19882121
Proc Natl Acad Sci U S A. 2004 Dec 7;101(49):17033-8
15563589
J Biol Chem. 1996 Apr 19;271(16):9273-80
8621588
J Exp Med. 2009 Jan 16;206(1):125-38
19103880
J Exp Med. 2009 Aug 3;206(8):1691-9
19596804
Blood. 2009 Apr 2;113(14):3333-6
19196661
Biochemistry (Mosc). 2006 May;71(5):496-504
16732727
Proc Natl Acad Sci U S A. 2004 Apr 20;101(16):6104-9
15079087
J Am Chem Soc. 2008 Apr 9;130(14):4578-9
18341338
J Biol Chem. 2007 Nov 2;282(44):32200-7
17728258
PLoS One. 2007 Feb 28;2(2):e257
17330143
Org Biomol Chem. 2007 Mar 21;5(6):865-72
17340000
Biochem J. 2001 Apr 15;355(Pt 2):489-97
11284738
Mol Cell Proteomics. 2010 Jun;9(6):1339-51
20172905
ACS Chem Biol. 2008 Sep 19;3(9):567-76
18729452
Nat Rev Immunol. 2007 Apr;7(4):255-66
17380156
Proc Natl Acad Sci U S A. 2009 Feb 24;106(8):2500-5
19202057
J Am Chem Soc. 2008 May 28;130(21):6680-1
18452295