The detection of Helicobacter pylori cag pathogenicity islands (PAIs) and expression of matrix metalloproteinase-7 (MMP-7) in gastric epithelial dysplasia and intramucosal cancer.
BACKGROUND
The cag pathogenicity island (PAI), a Helicobacter pylori virulence factor, is associated with the pathogenesis of gastric cancer. Matrix metalloproteinase-7(MMP-7) is upregulated in the epithelial cells of gastric cancer. To date, there is limited information available on the role of cag PAI and MMP-7 in precursor lesions. In this study, we aimed to identify virulent H. pylori strains and the expression of MMP-7 in samples of gastric epithelial dysplasia and intramucosal cancer.


METHODS
One hundred and twelve tissues excised by endoscopic mucosal resection, 76 specimens with gastric epithelial dysplasia and 36 with intramucosal cancer, were examined. All tissue samples were paired with surrounding normal epithelial tissue samples. We performed polymerase chain reaction for cagA and cagL in neoplasia and paired normal specimens, and assessed the matrix metalloproteinase (MMP)-7 expression by immunohistochemical staining.


RESULTS
There was a significant difference in the frequencies of cagA or cagL between specimens with gastric dysplasia and those with intramucosal cancer. We confirmed greater expression of MMP-7 immunoreactivity in intramucosal cancers infected with a virulent H. pylori strain.


CONCLUSIONS
Our results suggest that infection with a virulent H. pylori strain was associated with early-stage gastric cancer and that carcinogenesis was associated with cag PAI-dependent MMP-7 upregulation.
Chung
Woo Chul
WC
Department of Internal Medicine, College of Medicine, the Catholic University of Korea, Seoul, Korea.
Jung
Sung Hoon
SH
Lee
Kang-Moon
KM
Paik
Chang Nyol
CN
Kawk
Jea Wuk
JW
Jung
Ji Han
JH
Lee
Min Kyoung
MK
Lee
Yun Kyung
YK
D000942:Antigens, Bacterial / Q000235:genetics / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D005753:Gastric Mucosa / Q000201:enzymology / Q000473:pathology; D005773:Gastroscopy; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D044404:Genomic Islands; D006305:Health Status Indicators; D016481:Helicobacter Infections / Q000201:enzymology* / Q000382:microbiology / Q000473:pathology; D016480:Helicobacter pylori / Q000235:genetics / Q000472:pathogenicity*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D020783:Matrix Metalloproteinase 7 / Q000096:biosynthesis* / Q000235:genetics; D008875:Middle Aged; D012307:Risk Factors; D013274:Stomach Neoplasms / Q000201:enzymology* / Q000382:microbiology / Q000473:pathology; D014774:Virulence
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C080866:cagA protein, Helicobacter pylori; D020783:Matrix Metalloproteinase 7
N Engl J Med. 2001 Sep 13;345(11):784-9
11556297
Hum Pathol. 2008 Dec;39(12):1802-8
18715618
Gastroenterology. 2000 Jul;119(1):7-14
10889149
Diagn Microbiol Infect Dis. 2007 Sep;59(1):7-16
17521839
Br J Surg. 2005 Apr;92(4):454-62
15609380
Gastroenterology. 2006 May;130(6):1754-63
16697739
Cancer Res. 1995 May 15;55(10):2111-5
7743510
Lab Invest. 1995 Dec;73(6):760-70
8558837
J Pathol. 2006 Jan;208(2):233-48
16362989
World J Gastroenterol. 2005 Nov 21;11(43):6815-22
16425389
Infect Immun. 1995 May;63(5):1681-87
7729872
Dig Dis Sci. 1997 Aug;42(8):1652-9
9286230
Am J Surg Pathol. 1996 Oct;20(10):1161-81
8827022
Exp Biol Med (Maywood). 2006 Jan;231(1):20-7
16380641
Int J Biochem Cell Biol. 2008;40(6-7):1362-78
18258475
Gut. 1997 Mar;40(3):297-301
9135515
Gut. 1996 Sep;39(3):444-8
8949652
J Natl Cancer Inst. 1995 Dec 6;87(23):1777-80
7473834
Int J Oncol. 1998 Nov;13(5):1031-5
9772296
JAMA. 2004 Jan 14;291(2):187-94
14722144
Helicobacter. 2005 Dec;10(6):601-8
16302986
Matrix Biol. 2008 Apr;27(3):267-71
18023162
Clin Diagn Lab Immunol. 2001 Jan;8(1):181-6
11139216
Virchows Arch. 2003 Feb;442(2):99-106
12596058
J Formos Med Assoc. 2002 Jan;101(1):18-23
11911033
J Gastroenterol. 2004;39(5):434-40
15175941
Gut. 2003 Oct;52(10):1408-13
12970131
Eur Surg Res. 2007;39(4):208-15
17396032
J Cell Sci. 2003 Jul 15;116(Pt 14):3017-26
12808021
Nature. 2007 Oct 18;449(7164):862-6
17943123
Br J Surg. 1997 Feb;84(2):160-6
9052425
Gastroenterology. 2005 Jun;128(7):1937-52
15940628
Gut. 2004 Sep;53(9):1244-9
15306578
Cancer Sci. 2004 Nov;95(11):872-7
15546504
Int J Cancer. 1998 Apr 17;79(2):187-94
9583735
J Clin Oncol. 2000 Mar;18(5):1135-49
10694567
Nature. 2002 Dec 19-26;420(6917):860-7
12490959
Gastroenterology. 2003 Oct;125(4):1125-36
14517796
Mol Carcinog. 1991;4(6):527-33
1793490
Rev Esp Enferm Dig. 2001 Jul;93(7):471-80
11685943
Am J Surg Pathol. 1995;19 Suppl 1:S37-43
7762738
Mol Biol Cell. 2008 Oct;19(10):4110-21
18653469
J Formos Med Assoc. 2008 Aug;107(8):591-9
18678542
J Surg Oncol. 2006 May 1;93(6):491-7
16615152
J Clin Invest. 2004 Feb;113(3):321-33
14755326
J Clin Pathol. 1994 Oct;47(10):945-50
7962609