Multiple induced mutagenesis for improvement of ethanol production by Kluyveromyces marxianus.
Kluyveromyces marxianus GX-15 was mutated multiple times by alternately treatment with UV irradiation and NTG for two cycles. Four mutant strains with improved ethanol yield were obtained. The maximum ethanol concentration, ethanol yield coefficient and theoretical ethanol yield of the best mutant strain, GX-UN120, was 69 g/l, 0.46 g/g and 91%, respectively, when fermenting 150 g glucose/l at 40°C. The corresponding values for GX-15 were 58 g/l, 0.39 g/g and 76%, respectively. GX-UN120 grew well in 11% (v/v) of ethanol, while GX-15 could not grow when ethanol was greater than 8% (v/v).
Pang
Zong-wen
ZW
College of Life Science and Technology, Guangxi University, Nanning, Guangxi, 530005, China.
Liang
Jing-juan
JJ
Qin
Xiao-juan
XJ
Wang
Ji-rui
JR
Feng
Jia-xun
JX
Huang
Ri-bo
RB
D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D007716:Kluyveromyces / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism* / Q000528:radiation effects; D053858:Metabolic Networks and Pathways / Q000235:genetics*; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009153:Mutagens / Q000378:metabolism; D009604:Nitrosoguanidines / Q000378:metabolism; D017422:Sequence Analysis, DNA; D014466:Ultraviolet Rays
D009153:Mutagens; D009604:Nitrosoguanidines; D000431:Ethanol; D005947:Glucose