title: Fragmentation of the Golgi apparatus provides replication membranes for human rhinovirus 1A.
abstract: All viruses with a positive-stranded RNA genome replicate their genomic RNA in association with membranes from the host cell. Here we demonstrate a novel organelle source of replication membranes for human rhinovirus 1A (HRV-1A). HRV-1A infection induces fragmentation of the Golgi apparatus, and Golgi membranes are rearranged into vesicles of approximately 250-500 nm diameter. The newly distributed Golgi membranes co-localize with viral RNA replication templates, strongly suggesting that the observed vesicles are the sites of viral RNA replication. Expression of the HRV-1A 3A protein induces alterations in the Golgi staining pattern similar to those seen during viral infection, and expressed 3A localizes to the Golgi-derived membranes. Taken together, these data show that in HRV-1A infection, the 3A protein plays a role in fragmenting the Golgi complex and generating vesicles that are used as the site of viral RNA replication.
authors.affiliation: Department of Microbiology and Molecular Genetics and Center for Infectious Disease Research, Medical College of Wisconsin, Milwaukee, WI, USA.
authors.forename: Claire A
authors.initials: CA
authors.lastname: Quiner
authors.forename: William T
authors.initials: WT
authors.lastname: Jackson
mesh_terms: D002460:Cell Line; D006056:Golgi Apparatus / Q000378:metabolism* / Q000648:ultrastructure / Q000821:virology; D006367:HeLa Cells; D006801:Humans; D007425:Intracellular Membranes / Q000821:virology*; D012367:RNA, Viral / Q000378:metabolism; D012229:Rhinovirus / Q000502:physiology*; D014764:Viral Proteins / Q000378:metabolism*; D014779:Virus Replication
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins
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