Elr-type proteins protect Xenopus Dead end mRNA from miR-18-mediated clearance in the soma.
Segregation of the future germ line defines a crucial cell fate decision during animal development. In Xenopus, germ cells are specified by inheritance of vegetally localized maternal determinants, including a group of specific mRNAs. Here, we show that the vegetal localization elements (LE) of Xenopus Dead end (XDE) and of several other germ-line-specific, vegetally localized transcripts mediate germ cell-specific stabilization and somatic clearance of microinjected reporter mRNA in Xenopus embryos. The part of XDE-LE critical for somatic RNA clearance exhibits homology to zebrafish nanos1 and appears to be targeted by Xenopus miR-18 for somatic mRNA clearance. Xenopus Elr-type proteins of the vegetal localization complex can alleviate somatic RNA clearance of microinjected XDE-LE and endogenous XDE mRNA. ElrB1 synergizes with Xenopus Dead end protein in the stabilization of XDE-LE mRNA. Taken together, our findings unveil a functional link of vegetal mRNA localization and the protection of germ-line mRNAs from somatic clearance.
Koebernick
Katja
K
Department of Developmental Biochemistry, Göttingen Center for Molecular Biosciences, University of Göttingen, D-37077 Göttingen, Germany.
Loeber
Jana
J
Arthur
Patrick Kobina
PK
Tarbashevich
Katsiaryna
K
Pieler
Tomas
T
D000818:Animals; D001483:Base Sequence; D000067777:ELAV-Like Protein 2; D004625:Embryo, Nonmammalian / Q000378:metabolism; D005260:Female; D018507:Gene Expression Regulation, Developmental; D017930:Genes, Reporter; D005854:Germ Cells / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D012261:Ribonucleoproteins / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic; D029867:Xenopus Proteins / Q000235:genetics* / Q000378:metabolism; D014982:Xenopus laevis / Q000196:embryology* / Q000235:genetics / Q000378:metabolism*
C000624414:Dnd1 protein, Xenopus; D000067777:ELAV-Like Protein 2; C480172:ELAVL1 protein, Xenopus; D035683:MicroRNAs; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D012261:Ribonucleoproteins; D029867:Xenopus Proteins; C000593999:elavl2 protein, Xenopus
Nature. 2005 May 19;435(7040):360-4
15902260
Biol Cell. 2005 Jan;97(1):19-33
15601255
Cell. 2007 Dec 28;131(7):1273-86
18155131
Methods Mol Biol. 2006;342:129-38
16957372
Dev Biol. 2000 Jan 15;217(2):221-9
10625548
Mech Dev. 2006 Oct;123(10):746-60
16945508
Science. 2006 Apr 7;312(5770):75-9
16484454
Curr Biol. 2002 Oct 15;12(20):1756-61
12401170
Development. 1995 Feb;121(2):287-97
7539356
Mech Dev. 1998 May;73(2):159-68
9622619
Curr Biol. 2006 Nov 7;16(21):2135-42
17084698
Development. 1996 Dec;122(12):4119-29
9012531
Cell. 1985 Oct;42(3):769-77
2414011
Mech Dev. 1999 Jun;84(1-2):139-42
10473128
Curr Biol. 2003 Aug 19;13(16):1429-34
12932328
Genes Dev. 2002 Dec 1;16(23):3087-99
12464637
FEBS Lett. 2006 Sep 4;580(20):4947-52
16930598
Dev Biol. 2006 Mar 1;291(1):1-11
16448642
Development. 1998 Jan;125(2):171-80
9486791
Dev Cell. 2009 Apr;16(4):517-27
19386261
Curr Biol. 2008 Apr 8;18(7):501-6
18394895
Curr Top Dev Biol. 2000;50:155-81
10948454
Cell. 2006 Jun 16;125(6):1111-24
16777601
J Biol Chem. 2009 Jul 24;284(30):19982-92
19458392
Dev Biol. 2005 Nov 15;287(2):289-300
16216237
FEBS Lett. 2005 Jan 17;579(2):318-24
15642338
Int Rev Cytol. 2001;203:63-91
11131528
J Biol Chem. 2002 Nov 1;277(44):41539-46
12196519
PLoS One. 2009 Oct 19;4(10):e7513
19838299
Science. 2005 May 6;308(5723):833-8
15774722
Genome Biol. 2003;5(1):R1
14709173
RNA. 2009 Dec;15(12):2351-63
19854872