title: Atypical cadherins Dachsous and Fat control dynamics of noncentrosomal microtubules in planar cell polarity.
abstract: How global organ asymmetry and individual cell polarity are connected to each other is a central question in studying planar cell polarity (PCP). In the Drosophila wing, which develops PCP along its proximal-distal (P-D) axis, we previously proposed that the core PCP mediator Frizzled redistributes distally in a microtubule (MT)-dependent manner. Here, we performed organ-wide analysis of MT dynamics by introducing quantitative in vivo imaging. We observed MTs aligning along the P-D axis at the onset of redistribution and a small but significant excess of + ends-distal MTs in the proximal region of the wing. This characteristic alignment and asymmetry of MT growth was controlled by atypical cadherins Dachsous (Ds) and Fat (Ft). Furthermore, the action of Ft was mediated in part by PAR-1. All these data support the idea that the active reorientation of MT growth adjusts cell polarity along the organ axis.
authors.affiliation: Graduate School of Biostudies, Kyoto University, Yoshida Konoe-cho, Sakyo-ku, Kyoto 606-8507, Japan.
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Harumoto
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Ito
authors.forename: Yuko
authors.initials: Y
authors.lastname: Shimada
authors.forename: Tetsuya J
authors.initials: TJ
authors.lastname: Kobayashi
authors.forename: Hiroki R
authors.initials: HR
authors.lastname: Ueda
authors.forename: Bingwei
authors.initials: B
authors.lastname: Lu
authors.forename: Tadashi
authors.initials: T
authors.lastname: Uemura
mesh_terms: D000818:Animals; D015820:Cadherins / Q000502:physiology*; D015815:Cell Adhesion Molecules / Q000502:physiology*; D016764:Cell Polarity; D029721:Drosophila Proteins / Q000502:physiology*; D004331:Drosophila melanogaster / Q000196:embryology* / Q000235:genetics; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D038362:Glycogen Synthase Kinase 3; D008870:Microtubules / Q000378:metabolism*; D009024:Morphogenesis; D017346:Protein Serine-Threonine Kinases / Q000502:physiology; D015398:Signal Transduction; D013347:Subcellular Fractions; D014921:Wings, Animal / Q000196:embryology / Q000378:metabolism
chemical_list: D015820:Cadherins; D015815:Cell Adhesion Molecules; D029721:Drosophila Proteins; C094449:ds protein, Drosophila; C472307:ft protein, Drosophila; D017346:Protein Serine-Threonine Kinases; D038362:Glycogen Synthase Kinase 3; C472496:Par-1 protein, Drosophila
references.citation: Cell. 2001 Apr 20;105(2):209-19
references.pmid: 11336671
references.citation: Cell. 1997 Apr 18;89(2):297-308
references.pmid: 9108484
references.citation: Cell. 2008 Sep 5;134(5):843-53
references.pmid: 18775316
references.citation: J Cell Sci. 2006 Mar 15;119(Pt 6):979-87
references.pmid: 16525119
references.citation: J Cell Sci. 2006 Feb 15;119(Pt 4):711-21
references.pmid: 16449319
references.citation: Curr Biol. 2004 Apr 20;14(8):736-41
references.pmid: 15084291
references.citation: Trends Cell Biol. 2009 Jul;19(7):295-305
references.pmid: 19560358
references.citation: Nat Genet. 2008 Aug;40(8):1010-5
references.pmid: 18604206
references.citation: Mech Dev. 2000 Jun;94(1-2):95-109
references.pmid: 10842062
references.citation: Science. 2008 Jul 18;321(5887):401-4
references.pmid: 18635802
references.citation: Curr Biol. 2005 Sep 20;15(18):1640-4
references.pmid: 16169485
references.citation: Dev Cell. 2008 Sep;15(3):462-469
references.pmid: 18804440
references.citation: EMBO J. 2004 Jan 14;23(1):111-9
references.pmid: 14685271
references.citation: Dev Cell. 2002 Dec;3(6):851-63
references.pmid: 12479810
references.citation: J Exp Biol. 1966 Jun;44(3):507-22
references.pmid: 5962706
references.citation: Development. 2004 Aug;131(15):3785-94
references.pmid: 15240556
references.citation: Semin Cell Dev Biol. 2009 Oct;20(8):964-71
references.pmid: 19665570
references.citation: J Cell Biol. 1996 Dec;135(5):1277-89
references.pmid: 8947551
references.citation: Nature. 2003 Jan 30;421(6922):543-7
references.pmid: 12540853
references.citation: Cell. 2007 Jun 15;129(6):1051-63
references.pmid: 17574020
references.citation: EMBO J. 2004 Oct 27;23(21):4190-201
references.pmid: 15343271
references.citation: Cell. 1991 Aug 9;66(3):451-64
references.pmid: 1651169
references.citation: Cell. 2000 May 12;101(4):377-88
references.pmid: 10830165
references.citation: Dev Cell. 2005 Dec;9(6):805-17
references.pmid: 16326392
references.citation: Proc Natl Acad Sci U S A. 2008 Sep 30;105(39):14897-902
references.pmid: 18809931
references.citation: Proc Natl Acad Sci U S A. 2008 Dec 2;105(48):18800-5
references.pmid: 19022903
references.citation: Development. 2004 Feb;131(4):755-65
references.pmid: 14724126
references.citation: Cell. 1999 Sep 3;98(5):585-95
references.pmid: 10490098
references.citation: Proc Natl Acad Sci U S A. 2009 Jul 21;106(29):11989-94
references.pmid: 19574458
references.citation: Development. 2003 Sep;130(17):3965-75
references.pmid: 12874119
references.citation: PLoS One. 2008 Feb 13;3(2):e1600
references.pmid: 18270587
references.citation: Dev Biol. 1988 Oct;129(2):541-54
references.pmid: 3417051
references.citation: Dev Cell. 2007 Nov;13(5):609-622
references.pmid: 17981131
references.citation: Mol Cell. 2001 Feb;7(2):367-75
references.pmid: 11239465
references.citation: Development. 2006 Jun;133(12):2315-24
references.pmid: 16687445
references.citation: Development. 1999 Dec;126(23):5421-9
references.pmid: 10556066
references.citation: Dev Cell. 2006 Sep;11(3):375-85
references.pmid: 16908221
references.citation: Nature. 1989 Mar 16;338(6212):263-4
references.pmid: 2493583
references.citation: Development. 2004 Dec;131(24):6175-84
references.pmid: 15548581
references.citation: Curr Biol. 2010 May 11;20(9):811-7
references.pmid: 20434335
references.citation: Science. 2008 Dec 12;322(5908):1650-5
references.pmid: 19074340
references.citation: Cell. 2008 Jun 13;133(6):1093-105
references.pmid: 18555784
references.citation: Genes Dev. 1995 Jun 15;9(12):1530-42
references.pmid: 7601355
references.citation: Cell. 2002 May 3;109(3):371-81
references.pmid: 12015986
references.citation: Curr Biol. 2010 May 11;20(9):803-10
references.pmid: 20434337
references.citation: J Cell Sci. 2009 Jul 15;122(Pt 14):2351-9
references.pmid: 19531584
references.citation: Nat Rev Genet. 2007 Jul;8(7):555-63
references.pmid: 17563758
references.citation: Curr Biol. 2001 Jan 23;11(2):75-87
references.pmid: 11231123
references.citation: Cell. 2002 Mar 8;108(5):675-88
references.pmid: 11893338
references.citation: Curr Biol. 2004 Aug 24;14(16):1425-35
references.pmid: 15324659
references.citation: Curr Biol. 2008 Oct 28;18(20):1555-64
references.pmid: 18804371
references.citation: Curr Biol. 2008 Oct 14;18(19):R898-902
references.pmid: 18957230
references.citation: Dev Biol. 2007 Jun 15;306(2):624-35
references.pmid: 17475233
references.citation: Dev Cell. 2009 Jun;16(6):901-8
references.pmid: 19531360
references.citation: Semin Cell Dev Biol. 2009 Oct;20(8):957-63
references.pmid: 19751618
references.citation: Mech Dev. 2003 Nov;120(11):1257-64
references.pmid: 14623436
references.citation: Development. 2007 Feb;134(4):647-58
references.pmid: 17259302
references.citation: Curr Biol. 2009 Jul 14;19(13):1112-7
references.pmid: 19540118
references.citation: Dev Cell. 2006 Feb;10(2):209-22
references.pmid: 16459300
references.citation: Dev Biol. 1975 Mar;43(1):1-23
references.pmid: 807489
references.citation: Dev Cell. 2008 Aug;15(2):309-21
references.pmid: 18694569
references.citation: Mech Dev. 1998 Jan;70(1-2):181-92
references.pmid: 9510034
references.citation: Development. 1998 Mar;125(5):959-68
references.pmid: 9449678
references.citation: Science. 2002 Dec 6;298(5600):1955-9
references.pmid: 12471248
references.citation: Development. 2002 Jul;129(14):3493-503
references.pmid: 12091318
references.citation: Dev Cell. 2002 May;2(5):525-35
references.pmid: 12015961