title: Smad4 mediates malignant behaviors of human ovarian carcinoma cell through the effect on expressions of E-cadherin, plasminogen activator inhibitor-1 and VEGF.
abstract: Smad4 is involved in cancer progression and metastasis. Using a pair of human syngeneic epithelial ovarian cancer cells with low (HO-8910) and high (HO-8910PM) metastatic abilities, we aimed to reveal the role of Smad4 in ovarian cancer metastasis in vitro. Smad4 was down-regulated in HO-8910PM cell line relative to HO-8910 by implicating Smad4 was probably a potential tumor suppressor gene for ovarian cancer. Re-expression of Smad4 decreased the migration ability and inhibited the invasion capacity of HO-8910PM, while promoted the cell adhesion capacity for HO-8910PM. The stable expression of Smad4 increased the expression of E-cadherin, reduced the expression of plasminogen activator inhibitor-1 (PAI-1) and slightly down-regulated the expression of VEGF. Smad4 suppresses human ovarian cancer cell metastasis potential through its effect on the expressions of PAI-1, E-cadherin and VEGF. Results from current work implicate Smad4 might suppress the invasion and metastasis of human ovarian tumor cells through a TGF-Beta/Smad-mediated pathway.
authors.affiliation: Key Laboratory for Proteomics of Liaoning Province, Dalian Medical University, Dalian 116044, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Chen
authors.forename: Ming-Zhong
authors.initials: MZ
authors.lastname: Sun
authors.forename: Shuqing
authors.initials: S
authors.lastname: Liu
authors.forename: Dongmei
authors.initials: D
authors.lastname: Yeh
authors.forename: Lijun
authors.initials: L
authors.lastname: Yu
authors.forename: Yang
authors.initials: Y
authors.lastname: Song
authors.forename: Linlin
authors.initials: L
authors.lastname: Gong
authors.forename: Lihong
authors.initials: L
authors.lastname: Hao
authors.forename: Jun
authors.initials: J
authors.lastname: Hu
authors.forename: Shujuan
authors.initials: S
authors.lastname: Shao
mesh_terms: D015820:Cadherins / Q000235:genetics / Q000378:metabolism*; D002277:Carcinoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D002448:Cell Adhesion; D015536:Down-Regulation; D005260:Female; D006801:Humans; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D010051:Ovarian Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D017395:Plasminogen Activator Inhibitor 1 / Q000235:genetics / Q000378:metabolism*; D051901:Smad4 Protein / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D042442:Vascular Endothelial Growth Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D015820:Cadherins; D017395:Plasminogen Activator Inhibitor 1; C497796:SMAD4 protein, human; D051901:Smad4 Protein; D042442:Vascular Endothelial Growth Factors