title: Erythrocyte-protective effect of sarpogrelate hydrochloride (Anplag ®), a selective 5-HT2 receptor antagonist: an in vitro study.
abstract: Sarpogrelate hydrochloride (Anplag(®); Mitsubishi Tanabe Pharma Corporation, Osaka, Japan) is a selective S(2)-serotonergic receptor antagonist and is used as an antiplatelet agent. Sarpogrelate has been reported to increase erythrocyte deformability and suppress shear stress-induced hemolysis. In this study, we tested the protective effect of sarpogrelate on erythrocytes in an in vitro model. We compared the normalized index of hemolysis (NIH) between the group that was treated with sarpogrelate (n = 5) and the control group (n = 5) in a mock circulation under a shear force generated by a centrifugal pump. In the former group, 300 mg/day sarpogrelate was orally administered to a human volunteer for 2 days before blood collection. The NIH was significantly lower in the sarpogrelate group than it was in the control group (0.0012 ± 0.0009 vs. 0.0027 ± 0.0005 g/100 l, respectively; p = 0.016). Sarpogrelate suppressed shear stress-induced hemolysis in an in vitro model suggesting that sarpogrelate has a protective effect on erythrocytes under mechanical shear stress.
authors.affiliation: Department of Cardiac Surgery, Jikei University Kashiwa Hospital, 163-1 Kashiwa-shita, Kashiwa, Chiba, 277-8567, Japan. kenkentakota@yahoo.co.jp
authors.forename: Ken
authors.initials: K
authors.lastname: Nakamura
authors.forename: Koji
authors.initials: K
authors.lastname: Kawahito
mesh_terms: D002478:Cells, Cultured; D004912:Erythrocytes / Q000187:drug effects*; D006461:Hemolysis / Q000187:drug effects*; D006801:Humans; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology; D012702:Serotonin Antagonists / Q000494:pharmacology; D033081:Shear Strength / Q000187:drug effects; D013314:Stress, Mechanical; D013386:Succinates / Q000494:pharmacology*
chemical_list: D010975:Platelet Aggregation Inhibitors; D012702:Serotonin Antagonists; D013386:Succinates; C064294:sarpogrelate
references.citation: J Thorac Cardiovasc Surg. 1967 Jul;54(1):22-9
references.pmid: 6027856
references.citation: Thromb Haemost. 1991 Oct 1;66(4):484-8
references.pmid: 1796400
references.citation: Jpn J Thorac Cardiovasc Surg. 2000 Dec;48(12):769-74
references.pmid: 11197820
references.citation: J Heart Valve Dis. 2001 Jul;10(4):431-5
references.pmid: 11499585
references.citation: J Thorac Cardiovasc Surg. 2002 Mar;123(3):550-6
references.pmid: 11882830
references.citation: Artif Organs. 1997 Apr;21(4):323-6
references.pmid: 9096806
references.citation: Thromb Haemost. 1991 Apr 8;65(4):415-20
references.pmid: 2057925
references.citation: J Lab Clin Med. 1966 Jan;67(1):116-26
references.pmid: 5900720
references.citation: Artif Organs. 1996 Sep;20(9):1042-9
references.pmid: 8864026
references.citation: Am J Hematol. 2006 Mar;81(3):175-7
references.pmid: 16493616
references.citation: J Heart Valve Dis. 2008 Jan;17(1):24-30
references.pmid: 18365565
references.citation: Am J Cardiol. 1993 Mar 1;71(7):587-91
references.pmid: 8438746