[Study on the function of plasmid in pyridine-degrading bacteria].
In order to identify the characteristics of the plasmids of degrading bacterial strains and the relationship between the plasmids' function and biodegradation, plasmids were isolated from two bacterial strains (Paracoccus sp. BW001 and Shinella zoogloeoides BC026) and pulse-field gel electrophoresis was used to identify the distribution of plasmids and their molecular size. Two large plasmids with 190-245 kb and one small plasmid with 4.5-5.0 kb were found in the BW001, and at least 3 large plasmids over 200 kb were harbored in the BC026. The plasmid curing was conducted by high temperature-SDS method and the results indicated the biodegradation genes might locate in the plasmids of two bacterial strains. After transforming the plasmids of BW001 into E. coli 5alpha by electroporation, the new bacterial strain could tolerate pyridine.
Bai
Yao-hui
YH
College of Environmental Sciences and Engineering,Peking University, Beijing 100871, China. baiyh@pku.edu.cn
Sun
Qing-hua
QH
Zhao
Cui
C
Wen
Dong-hui
DH
Tang
Xiao-yan
XY
D001673:Biodegradation, Environmental; D018274:Electroporation; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D010230:Paracoccus / Q000235:genetics* / Q000502:physiology; D010957:Plasmids / Q000235:genetics / Q000502:physiology*; D011725:Pyridines / Q000302:isolation & purification* / Q000378:metabolism; D012230:Rhizobiaceae / Q000235:genetics* / Q000502:physiology; D014865:Waste Disposal, Fluid / Q000379:methods; D014874:Water Pollutants, Chemical / Q000302:isolation & purification* / Q000378:metabolism
D011725:Pyridines; D014874:Water Pollutants, Chemical; C023666:pyridine