Association between the -1562 C/T MMP-9 polymorphism and cerebrovascular disease in a Polish population.
OBJECTIVE
Matrix metalloproteinase 9 (MMP-9) is an endopeptidase degrading extracellular matrix. There is growing evidence that changes in extracellular matrix play an important role in vascular pathology, especially in cardiovascular and cerebrovascular disease. Previous studies have demonstrated that MMP-9 activity is controlled by --1562 C/T polymorphism. Genotypes with T allele (CT, TT) have higher enzymatic activity. Thus, this polymorphism could be responsible for the higher risk for cerebrovascular disease and death. The aim of this study was to assess the significance of MMP-9 polymorphism as a risk factor for cerebrovascular disease in a Polish population.


METHODS
A total of 775 consecutive patients with a diagnosis of cerebrovascular disease (ischaemic stroke, intracerebral haemorrhage, subarachnoid haemorrhage) admitted to the Stroke Unit, Jagiellonian University, Krakow, Poland between 2000 and 2004 were studied and compared with 766 matched controls. The polymorphism was studied by polymerase chain reaction (PCR) and restricted enzyme digestion.


RESULTS
Among 418 patients with ischaemic stroke of various aetiologies and among 146 patients with primary intracerebral haemorrhage and 211 patients with subarachnoid haemorrhage due to ruptured intracranial aneurysm, statistical analysis did not show a significant difference between occurrence of CC, CT, TT genotypes or C and T alleles in patients with stroke of various aetiology compared with controls.


CONCLUSIONS
We found no association between the -1562 C/T MMP-9 polymorphism and ischaemic stroke, subarachnoid haemorrhage or spontaneous intracerebral haemorrhage in the studied Polish population.
Szczudlik
Piotr
P
Klinika Neurologii, Warszawski Uniwersytet Medyczny, ul. Banacha 1a, 00-097 Warszawa, Poland. pszczudlik@wum.edu.pl
Borratyńska
Anna
A
D000368:Aged; D000369:Aged, 80 and over; D001161:Arteriosclerosis / Q000235:genetics; D002545:Brain Ischemia / Q000235:genetics; D002340:Carotid Artery Diseases / Q000235:genetics; D002561:Cerebrovascular Disorders / Q000453:epidemiology / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease / Q000453:epidemiology; D006801:Humans; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000235:genetics*; D008875:Middle Aged; D011044:Poland / Q000453:epidemiology; D011110:Polymorphism, Genetic; D011237:Predictive Value of Tests; D018570:Risk Assessment; D012307:Risk Factors
D020780:Matrix Metalloproteinase 9