Relationship between proteome changes of Longissimus muscle and intramuscular fat content in finishing pigs fed conjugated linoleic acid.
The present experiment was conducted to determine proteome changes in Longissimus muscle of finishing pigs fed conjugated linoleic acid (CLA), in association with alteration of intramuscular fat content. Previously, seventy-two Duroc × Landrace × Large White gilts (approximately 60 kg) had been fed maize–soyabean meal-based diets with 0, 12·5 and 25 g CLA/kg diet. The CLA contained 369·1 mg/g cis-9, trans-11 CLA, 374·6 mg/g trans-10, cis-12 CLA and 53·7 mg/g other isomers. Six pigs per treatment were slaughtered when they reached a body weight of approximately 100 kg. Data published from a previous experiment demonstrated that supplementation with 12·5 or 25 g CLA/kg diet increased intramuscular fat content (P < 0·05). The present study investigated the proteome changes in Longissimus muscle of control and pigs supplemented with 25 g CLA/kg diet. CLA significantly influenced the abundance of proteins related to energy metabolism, fatty acid oxidation and synthesis, amino acid metabolism, defence, transport and other miscellaneous processes (P < 0·05). The increase in intramuscular fat content was positively correlated with the increased abundance of carbonic anhydrase 3 and aspartate aminotransferase (P < 0·05). We suggest that the proteome changes in Longissimus muscle contributed to greater intramuscular lipid content in CLA-supplemented pigs.
Zhong
Weijing
W
Ministry of Agriculture Key Laboratory of Animal Nutrition and Feed Science (South China), State Key Laboratory of Livestock and Poultry Breeding, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, People's Republic of China.
Jiang
Zongyong
Z
Zheng
Chuntian
C
Lin
Yingcai
Y
Yang
Lin
L
Zou
Shutong
S
D000273:Adipose Tissue / Q000378:metabolism*; D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism; D002256:Carbonic Anhydrases / Q000378:metabolism; D004041:Dietary Fats / Q000008:administration & dosage / Q000378:metabolism*; D019587:Dietary Supplements; D004734:Energy Metabolism; D005227:Fatty Acids / Q000378:metabolism; D005260:Female; D044243:Linoleic Acids, Conjugated / Q000378:metabolism / Q000494:pharmacology*; D050356:Lipid Metabolism; D015227:Lipid Peroxidation; D008460:Meat; D009124:Muscle Proteins / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000378:metabolism*; D013552:Swine / Q000378:metabolism
D000596:Amino Acids; D004041:Dietary Fats; D005227:Fatty Acids; D044243:Linoleic Acids, Conjugated; D009124:Muscle Proteins; D001219:Aspartate Aminotransferases; D002256:Carbonic Anhydrases