title: Profiling of phenols in human fecal water after raspberry supplementation.
abstract: The phenolic compositions of fecal water samples from ten free-living human subjects without marked dietary restrictions were monitored before and after intake of raspberry puree (200 g/day, 4 days) using gas chromatography-mass spectrometry. No single phenolic component was increased in all subjects after intake, but a majority of subjects had significant elevations in phenylacetic acid (7/10), 4-hydroxyphenylacetic acid (6/10), 3-hydroxyphenylacetic acid (5/10), 3-phenylpropionic acid and 3-(4-hydroxyphenyl)propionic acid. The levels of 3,4-dihydroxbenzoic acid were elevated in 8/10 subjects, significantly for 6 subjects (p < 0.05), and not significantly reduced in the other 2 subjects. In addition, unlike most other fecal metabolites, the increase was always >2-fold. This metabolite may be representative of the increased colonic dose of cyanidin anthocyanins. The colonic microbiota varied greatly between individuals, and supplementation with raspberries did not produce any statistically significant alterations in the profile of colonic bacteria, nor was a common pattern revealed to account for the interindividual variations observed in the fecal water phenolic profiles.
authors.affiliation: Northern Ireland Centre for Food and Health, Centre for Molecular Biosciences, University of Ulster, Cromore Road, Coleraine, N Ireland BT52 1SA, UK. c.gill@ulster.ac.uk
authors.forename: Chris I R
authors.initials: CI
authors.lastname: Gill
authors.forename: Gordon J
authors.initials: GJ
authors.lastname: McDougall
authors.forename: Sheila
authors.initials: S
authors.lastname: Glidewell
authors.forename: Derek
authors.initials: D
authors.lastname: Stewart
authors.forename: Qing
authors.initials: Q
authors.lastname: Shen
authors.forename: Kieran
authors.initials: K
authors.lastname: Tuohy
authors.forename: Aine
authors.initials: A
authors.lastname: Dobbin
authors.forename: Adele
authors.initials: A
authors.lastname: Boyd
authors.forename: Emma
authors.initials: E
authors.lastname: Brown
authors.forename: Sumanto
authors.initials: S
authors.lastname: Haldar
authors.forename: Ian R
authors.initials: IR
authors.lastname: Rowland
mesh_terms: D000328:Adult; D001834:Body Water / Q000737:chemistry*; D004032:Diet; D005243:Feces / Q000737:chemistry* / Q000382:microbiology; D005419:Flavonoids / Q000032:analysis; D005638:Fruit / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D008297:Male; D008875:Middle Aged; D010636:Phenols / Q000032:analysis*; D010648:Phenylacetates / Q000032:analysis; D059808:Polyphenols; D027824:Rosaceae / Q000737:chemistry*
chemical_list: D005419:Flavonoids; D010636:Phenols; D010648:Phenylacetates; D059808:Polyphenols; C025136:phenylacetic acid