title: Large-scale extraction of recombinant proteins from bacteria.
abstract: Bacteria are particularly convenient for producing recombinant proteins for purification purposes. Suitable extraction methods for bacterial cells include sonication, glass bead milling, grinding with alumina or sand, high-pressure shearing using the French pressure cell (French Press), and lysozyme treatment. These procedures are applicable for preparing extracts from a variety of gram-negative bacteria such as Escherichia coli and Klebsiella pneumoniae, and gram-positive bacteria such as Bacillus subtilis. Disruption of bacterial cells by enzymatic means is commonly used because a relatively uniform treatment is obtained when cells are in suspension. This protocol describes the enzymatic disruption of E. coli.
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Simpson
mesh_terms: D001433:Bacteriolysis; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D009113:Muramidase / Q000378:metabolism; D015202:Protein Engineering / Q000379:methods; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D011994:Recombinant Proteins; D009113:Muramidase