title: Membrane perturbation induced by papiliocin peptide, derived from Papilio xuthus, in Candida albicans.
abstract: Previously, papiliocin was isolated from the swallowtail butterfly Papilio xuthus and its antimicrobial activity was suggested. In this study, the antifungal mechanism of papiliocin was investigated against Candida albicans. Confocal laser scanning microscopy (CLSM) and 1,6-diphenyl-1,3,5-hexatriene (DPH) fluorescence analysis indicated that papiliocin disturbed the fungal plasma membrane. Moreover, the assessment of the release of FITC-dextran (FD) from liposome further demonstrated that the antifungal mechanism of papiliocin could have originated from the pore-forming action and that the radius of the pores was presumed to be anywhere from 2.3 nm and 3.3 nm.
authors.affiliation: School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University, Daegu, Korea.
authors.forename: Juneyoung
authors.initials: J
authors.lastname: Lee
authors.forename: Jae-Sam
authors.initials: JS
authors.lastname: Hwang
authors.forename: Bomi
authors.initials: B
authors.lastname: Hwang
authors.forename: Jin-Kyoung
authors.initials: JK
authors.lastname: Kim
authors.forename: Seong Ryul
authors.initials: SR
authors.lastname: Kim
authors.forename: Yangmee
authors.initials: Y
authors.lastname: Kim
authors.forename: Dong Gun
authors.initials: DG
authors.lastname: Lee
mesh_terms: D000818:Animals; D000935:Antifungal Agents / Q000378:metabolism / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides / Q000378:metabolism / Q000494:pharmacology*; D002080:Butterflies / Q000737:chemistry* / Q000378:metabolism; D002176:Candida albicans / Q000737:chemistry / Q000187:drug effects*; D002462:Cell Membrane / Q000737:chemistry / Q000187:drug effects*
chemical_list: D000935:Antifungal Agents; D023181:Antimicrobial Cationic Peptides; C551727:papiliocin protein, Papilio xuthus