title: Migratory route of Strongyloides venezuelensis in Lewis rats: comparison of histological analyses and PCR.
abstract: Strongyloides venezuelensis is a parasitic nematode that has been used as a model to study human and animal strongyloidiasis. In this study, we compared the sensitivity between traditional methodologies and PCR assay to characterize the dynamics of S. venezuelensis infection and its migration route in Lewis rats subcutaneously infected with 4000 L3. The dynamics of the infection was determined by counting the number of eggs and by detecting parasite deoxyribonucleic acid in faeces samples. Both techniques similarly detected the infection at day 6 after larvae inoculation. However, PCR performed with the genus primer showed higher sensitivity during the recovery phase. Histological analysis and PCR assay were then used to follow parasite tissue migration. S. venezuelensis migration route included the muscular fibers below the skin, the pulmonary alveoli and the small intestine vilosities. The sensitivity of these two techniques to detect parasite's presence in these tissues was statistically similar.
authors.affiliation: Department of Parasitology, Biosciences Institute, Univ Estadual Paulista, Botucatu, São Paulo, Brazil.
authors.forename: Nelson Mendes
authors.initials: NM
authors.lastname: Marra
authors.forename: Fernanda
authors.initials: F
authors.lastname: Chiuso-Minicucci
authors.forename: Gabriel Capella
authors.initials: GC
authors.lastname: Machado
authors.forename: Sofia Fernanda Gonçalves
authors.initials: SF
authors.lastname: Zorzella-Pezavento
authors.forename: Thaís Graziela Donegá
authors.initials: TG
authors.lastname: França
authors.forename: Larissa Lumi Watanabe
authors.initials: LL
authors.lastname: Ishikawa
authors.forename: Alessandro F T
authors.initials: AF
authors.lastname: Amarante
authors.forename: Alexandrina
authors.initials: A
authors.lastname: Sartori
authors.forename: Mônica R V
authors.initials: MR
authors.lastname: Amarante
mesh_terms: D000818:Animals; D001921:Brain / Q000469:parasitology; D018509:DNA, Helminth / Q000032:analysis; D005243:Feces / Q000469:parasitology; D005260:Female; D007421:Intestine, Small / Q000469:parasitology; D008168:Lung / Q000469:parasitology; D008297:Male; D018482:Muscle, Skeletal / Q000469:parasitology; D010270:Parasite Egg Count; D016133:Polymerase Chain Reaction; D051381:Rats; D011917:Rats, Inbred Lew; D012680:Sensitivity and Specificity; D012867:Skin / Q000469:parasitology; D013321:Strongyloides / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D013322:Strongyloidiasis / Q000469:parasitology*; D013997:Time Factors; D014781:Viscera / Q000469:parasitology
chemical_list: D018509:DNA, Helminth