title: BRCC36A is epistatic to BRCA1 in DNA crosslink repair and homologous recombination in Arabidopsis thaliana.
abstract: BRCA1 is a well-known tumor suppressor protein in mammals, involved in multiple cellular processes such as DNA repair, chromosome segregation and chromatin remodeling. Interestingly, homologs of BRCA1 and several of its complex partners are also found in plants. As the respective mutants are viable, in contrast to mammalian mutants, detailed analyses of their biological role is possible. Here we demonstrate that the model plant Arabidopsis thaliana harbors two homologs of the mammalian BRCA1 interaction partner BRCC36, AtBRCC36A and AtBRCC36B. Mutants of both genes as well as the double mutants are fully fertile and show no defects in development. We were able to show that mutation of one of the homologs, AtBRCC36A, leads to a severe defect in intra- and interchromosomal homologous recombination (HR). A HR defect is also apparent in Atbrca1 mutants. As the Atbrcc36a/Atbrca1 double mutant behaves like the single mutants of AtBRCA1 and AtBRCC36A both proteins seem to be involved in a common pathway in the regulation of HR. AtBRCC36 is also epistatic to AtBRCA1 in DNA crosslink repair. Upon genotoxic stress, AtBRCC36A is transferred into the nucleus.
authors.affiliation: Karlsruhe Institute of Technology, Botanical Institute II, Karlsruhe, Germany.
authors.forename: Astrid S
authors.initials: AS
authors.lastname: Block-Schmidt
authors.forename: Stefanie
authors.initials: S
authors.lastname: Dukowic-Schulze
authors.forename: Kristina
authors.initials: K
authors.lastname: Wanieck
authors.forename: Wim
authors.initials: W
authors.lastname: Reidt
authors.forename: Holger
authors.initials: H
authors.lastname: Puchta
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D019313:BRCA1 Protein / Q000235:genetics / Q000502:physiology*; D001761:Bleomycin / Q000633:toxicity; D004260:DNA Repair; D004843:Epistasis, Genetic; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000032:analysis; D011995:Recombination, Genetic; D017386:Sequence Homology, Amino Acid
chemical_list: D029681:Arabidopsis Proteins; D019313:BRCA1 Protein; C555733:BRCC36A protein, Arabidopsis; C555734:BRCC36B protein, Arabidopsis; D009687:Nuclear Proteins; D001761:Bleomycin
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