Characterization of chromosomal rearrangements using multicolor-banding (MCB/m-band).
Molecular cytogenetics and especially fluorescence in situ hybridization (FISH) banding approaches are nowadays standard for the exact characterization of simple, complex, and cryptic chromosomal aberrations within the human genome. FISH-banding techniques are any kind of FISH techniques, which provide the possibility to characterize simultaneously several chromosomal subregions smaller than a chromosome arm. FISH banding methods fitting that definition may have quite different characteristics, but share the ability to produce a DNA-specific chromosomal banding. While the standard techniques such as G-bands by Trypsin using Giemsa banding lead to a protein-related black and white banding pattern, FISH-banding techniques are DNA-specific, more colorful, and thus, more informative. At present, the most frequently applied FISH banding technique is the multicolor banding (MCB/m-band) approach. MCB/m-band is based on region-specific microdissection libraries, producing changing fluorescence intensity ratios along the chromosomes. Here we describe the FISH-banding technique MCB/m-band and illustrate how to apply it for characterization of chromosomal breakpoints with a minimal number of FISH experiments.
Liehr
Thomas
T
Institut für Humangenetik und Anthropologie, University of Jena, Jena, Germany. i8lith@mti.uni-jena.de
Weise
Anja
A
Hinreiner
Sophie
S
Mkrtchyan
Hasmik
H
Mrasek
Kristin
K
Kosyakova
Nadezda
N
D002875:Chromosomes / Q000235:genetics*; D003116:Color; D004247:DNA / Q000235:genetics / Q000378:metabolism; D005456:Fluorescent Dyes / Q000378:metabolism; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D009691:Nucleic Acid Denaturation
D005456:Fluorescent Dyes; D004247:DNA