title: In vitro hemocompatibility of thin film nitinol in stenotic flow conditions.
abstract: Because of its low profile and biologically inert behavior, thin film nitinol (TFN) is ideally suited for use in construction of endovascular devices. We have developed a surface treatment for TFN designed to minimize platelet adhesion by creating a superhydrophilic surface. The hemocompatibility of expanded polytetrafluorethylene (ePTFE), untreated thin film nitinol (UTFN), and a surface treated superhydrophilic thin film nitinol (STFN) was compared using an in vitro circulation model with whole blood under flow conditions simulating a moderate arterial stenosis. Scanning electron microscopy analysis showed increased thrombus on ePTFE as compared to UTFN or STFN. Total blood product deposition was 6.3 ± 0.8 mg/cm(2) for ePTFE, 4.5 ± 2.3 mg/cm(2) for UTFN, and 2.9 ± 0.4 mg/cm(2) for STFN (n = 12, p < 0.01). ELISA assay for fibrin showed 326 ± 42 μg/cm(2) for ePTFE, 45.6 ± 7.4 μg/cm(2) for UTFN, and 194 ± 25 μg/cm(2) for STFN (n = 12, p < 0.01). Platelet deposition measured by fluorescent intensity was 79,000 20,000 AU/mm(2) for ePTFE, 810 ± 190 AU/mm(2) for UTFN, and 1600 ± 25 AU/mm(2) for STFN (n = 10, p < 0.01). Mass spectrometry demonstrated a larger number of proteins on ePTFE as compared to either thin film. UTFN and STFN appear to attract significantly less thrombus than ePTFE. Given TFN's low profile and our previously demonstrated ability to place TFN covered stents in vivo, it is an excellent candidate for use in next-generation endovascular stents grafts.
authors.affiliation: Department of Surgery, Division of Vascular Surgery, David Geffen School of Medicine, University of California, Los Angeles, CA, USA. ckealey@mednet.ucla.edu
authors.forename: C P
authors.initials: CP
authors.lastname: Kealey
authors.forename: S A
authors.initials: SA
authors.lastname: Whelan
authors.forename: Y J
authors.initials: YJ
authors.lastname: Chun
authors.forename: C H
authors.initials: CH
authors.lastname: Soojung
authors.forename: A W
authors.initials: AW
authors.lastname: Tulloch
authors.forename: K P
authors.initials: KP
authors.lastname: Mohanchandra
authors.forename: D
authors.initials: D
authors.lastname: Di Carlo
authors.forename: D S
authors.initials: DS
authors.lastname: Levi
authors.forename: G P
authors.initials: GP
authors.lastname: Carman
authors.forename: D A
authors.initials: DA
authors.lastname: Rigberg
mesh_terms: D000497:Alloys / Q000494:pharmacology*; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism; D001798:Blood Proteins / Q000737:chemistry / Q000378:metabolism; D023921:Coronary Stenosis / Q000503:physiopathology*; D005337:Fibrin / Q000378:metabolism; D018056:Hemorheology / Q000187:drug effects*; D006801:Humans; D013058:Mass Spectrometry; D008422:Materials Testing / Q000379:methods*; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D013927:Thrombosis / Q000473:pathology
chemical_list: D000497:Alloys; D001798:Blood Proteins; C013616:nitinol; D005337:Fibrin
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