title: [Construction of recombinant adenovirus containing TK gene and its effect against human liver cancer cells].
abstract: OBJECTIVE
  To construct a replication-defective adenovirus containing TK gene and investigate the killing effects of TK gene against human liver cancer cells SMMC-7721.
  
  
  METHODS
  The recombinant adenovirus ADV-TK was constructed using homologous recombination in the cells. SMMC-7721 cells transfected with recombined adenovirus were exposed to GCV, and the cell viability was measured by MTT assays.
  
  
  RESULTS
  The recombinant adenovirus containing TK gene was successfully constructed. Transfection by the recombinant adenovirus ADV-TK and GCV exposure significantly suppressed the growth of SMMC-7721 cells.
  
  
  CONCLUSIONS
  A replication-defective adenovirus containing TK gene has been successfully constructed, and in combination with GCV, the recombinant adenovirus produces significant killing effect against SMMC-7721 cells in vitro.
authors.affiliation: Third Department of Surgery, Second Affiliated Hospital, Shantou University Medical College, Shantou 515041, China. chengengzhen@tom.com
authors.forename: Geng-zhen
authors.initials: GZ
authors.lastname: Chen
authors.forename: Hui
authors.initials: H
authors.lastname: Hu
authors.forename: Ming-yan
authors.initials: MY
authors.lastname: Xu
authors.forename: Xiao-ling
authors.initials: XL
authors.lastname: Deng
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D045744:Cell Line, Tumor; D015316:Genetic Therapy; D005822:Genetic Vectors; D006801:Humans; D008113:Liver Neoplasms / Q000628:therapy*; D013937:Thymidine Kinase / Q000235:genetics
chemical_list: D013937:Thymidine Kinase