title: Fluorinated diaminocyclopentanes as chiral sensitive NMR probes of RNA structure.
abstract: The supramolecular chiral recognition between rac-2a and several structured RNA leads to a distinct (19)F NMR signal splitting. The (19)F NMR analysis of the diastereomeric pairs formed upon binding of this racemic probe delivers a topological footprint of the RNA. This phenomenon can be exploited to investigate dynamic events involving structural equilibria, as demonstrated in a melting experiment. This work provides a proof of concept that small fluorinated moderate binders can act as external probes of RNA structures.
authors.affiliation: UMR8638 and UMR8015, CNRS-Paris Descartes University, Faculté de Pharmacie, 4 av. de l'Observatoire, 75006 Paris, France.
authors.forename: Roba
authors.initials: R
authors.lastname: Moumné
authors.forename: Morgane
authors.initials: M
authors.lastname: Pasco
authors.forename: Elise
authors.initials: E
authors.lastname: Prost
authors.forename: Thomas
authors.initials: T
authors.lastname: Lecourt
authors.forename: Laurent
authors.initials: L
authors.lastname: Micouin
authors.forename: Carine
authors.initials: C
authors.lastname: Tisné
mesh_terms: D001483:Base Sequence; D003517:Cyclopentanes / Q000737:chemistry*; D005461:Fluorine / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D015335:Molecular Probes; D009690:Nucleic Acid Conformation; D012313:RNA / Q000737:chemistry*
chemical_list: D003517:Cyclopentanes; D015335:Molecular Probes; D005461:Fluorine; D012313:RNA