Inhibitory effects of zingerone, a pungent component of Zingiber officinale Roscoe, on colonic motility in rats.
Ginger (rhizome of Zingiber officinale Roscoe) is an herbal medicine for the treatment of gastrointestinal disorders including constipation and diarrhea. Zingerone is a likely active constituent responsible for the antidiarrheal activity of ginger. The current study was designed to characterize pharmacological actions of zingerone on colonic motility. To evaluate pharmacological effects of zingerone on colonic motility, we used isolated colonic segments from rats, in which mechanical responses were recorded in the longitudinal direction. In addition, we evaluated the effects on colonic motility in vivo by measuring intraluminal pressure changes and expelled fluid volume from the colon in anesthetized rats. Zingerone was applied to the lumen of the colon to allow the drug to access from the mucosal side. Zingerone inhibited spontaneous contractile movements in the isolated colonic segments in a dose-dependent manner. The inhibitory effects of zingerone on colonic movements were not affected by pretreatment with capsazepine, a typical antagonist of transient receptor potential vanilloid 1. In addition, tetrodotoxin, a blocker of voltage-dependent sodium channels on neurons, did not affect the suppression of colonic movements by zingerone, suggesting that zingerone acts on the smooth muscles directly. Zingerone also attenuated colonic motility in vivo without affecting blood pressure and heart rate. The effects were reversible and reproducible. Our findings suggest that zingerone can inhibit colonic motility via direct action on smooth muscles. Zingerone might exert beneficial therapeutic effects on hypermotility-induced diarrhea by abrogating excessive gastrointestinal motility.
Iwami
Momoe
M
Laboratory of Physiology, Department of Basic Veterinary Science, The United Graduate School of Veterinary Sciences, 1-1 Yanagido, Gifu 501-1193, Japan.
Shiina
Takahiko
T
Hirayama
Haruko
H
Shima
Takeshi
T
Takewaki
Tadashi
T
Shimizu
Yasutake
Y
D000818:Animals; D003106:Colon / Q000187:drug effects*; D005769:Gastrointestinal Motility / Q000187:drug effects*; D020939:Zingiber officinale / Q000737:chemistry*; D006139:Guaiacol / Q000031:analogs & derivatives* / Q000494:pharmacology; D066298:In Vitro Techniques; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar
C013738:zingerone; D006139:Guaiacol
Pharmacol Rev. 1999 Jun;51(2):159-212
10353985
Pharmacology. 1999 Oct;59(4):212-20
10474081
Am J Chin Med. 2001;29(1):111-8
11321468
Biol Pharm Bull. 2004 Jul;27(7):1046-51
15256738
Life Sci. 2001 Apr 13;68(21):2347-60
11350006
Am J Physiol Gastrointest Liver Physiol. 2001 May;280(5):G949-57
11292604
Br J Pharmacol. 2003 Sep;140(2):231-8
12970093
J Ethnopharmacol. 2006 Oct 11;107(3):342-8
16713155
Neurogastroenterol Motil. 2004 Dec;16(6):701-11
15601419
Eur J Pharmacol. 1979 Apr 15;55(2):181-7
456415
J Agric Food Chem. 2007 Oct 17;55(21):8390-7
17880155
Eur J Pharmacol. 2006 Jan 13;530(1-2):144-51
16360146
Am J Physiol. 1986 Jan;250(1 Pt 1):G1-8
2867681
J Ethnopharmacol. 1989 Nov;27(1-2):129-40
2615416
Fitoterapia. 2007 Apr;78(3):171-4
17275209
Physiol Rev. 2002 Jan;82(1):245-89
11773614
Eur J Pharmacol. 1990 Sep 4;186(1):71-7
1704311
Crit Rev Food Sci Nutr. 1982;17(1):1-96
7049579
Food Chem Toxicol. 2008 Feb;46(2):409-20
17950516
J Physiol Paris. 2001 Jan-Dec;95(1-6):181-8
11595435
Gut. 1991 Nov;32(11):1355-9
1752468
Eur J Pharmacol. 2004 Mar 8;487(1-3):175-82
15033390
J Ethnopharmacol. 1996 Sep;53(3):157-63
8887023
Biol Pharm Bull. 2001 Jun;24(6):623-7
11411548
Adv Ther. 1998 Jan-Feb;15(1):25-44
10178636
Eur J Biochem. 1998 Jan 15;251(1-2):262-8
9492292
Curr Neuropharmacol. 2008 Mar;6(1):79-96
19305789
Dig Dis Sci. 2005 Oct;50(10):1889-97
16187193
Food Chem Toxicol. 2007 May;45(5):683-90
17175086
J Neurophysiol. 1996 Sep;76(3):1858-69
8890298
Life Sci. 2004 Apr 23;74(23):2889-96
15050426
Regul Pept. 2009 Jun 5;155(1-3):11-7
19345246
Aliment Pharmacol Ther. 2001 Sep;15(9):1239-52
11552894
Physiol Behav. 2000 Apr 1-15;69(1-2):177-86
10854928
Pharmacol Rev. 2005 Sep;57(3):315-38
16109838
Am J Physiol Gastrointest Liver Physiol. 2009 Aug;297(2):G348-60
19497956