Laminin-3B11, a novel vascular-type laminin capable of inducing prominent lamellipodial protrusions in microvascular endothelial cells.
The basement membrane (BM) proteins laminins, which consist of α, β, and γ chains, support tissue structures and cellular functions. To date only α4 and α5 types of laminins have been identified in the BMs of blood vessels. Our recent study suggested the presence of novel α3B-containing laminins in vascular BMs. Here we identified and characterized the third member of vascular laminins, laminin-3B11 (Lm3B11). RT-PCR analysis showed that microvascular endothelial (MVE) cells and umbilical vein endothelial cells expressed the messages for the α3B, β1, β2, and γ1 chains. In the culture of MVE cells, α3B was associated with β1 and γ1, producing Lm3B11. Recombinant Lm3B11 was overexpressed by introducing the cDNAs of the three chains into HEK-293 cells and purified to homogeneity. Purified Lm3B11 exhibited relatively weak cell adhesion activity through both α3β1 and α6β1 integrins. Most characteristically, Lm3B11 strongly stimulated MVE cells to extend many lamellipodial protrusions. This pseudopodial branching was blocked by an inhibitor for Src or phosphatidylinositol 3-kinase. Consistently, Lm3B11 stimulated the phosphorylation of Src and Akt more strongly than other laminins, suggesting that the integrin-derived signaling is mediated by these factors. The unique activity of Lm3B11 appears to be favorable to the branching of capillaries and venules.
Mori
Taizo
T
Graduate School of Integrated Sciences, Kihara Institute for Biological Research, Yokohama City University, 641-12 Maioka-cho, Totsuka-ku, Yokohama 244-0813, Japan.
Ono
Kota
K
Kariya
Yoshinobu
Y
Ogawa
Takashi
T
Higashi
Shouichi
S
Miyazaki
Kaoru
K
D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D055806:Microvessels / Q000166:cytology / Q000378:metabolism*; D015688:Oncogene Protein pp60(v-src) / Q000235:genetics / Q000378:metabolism; D010766:Phosphorylation / Q000502:physiology; D051057:Proto-Oncogene Proteins c-akt / Q000235:genetics / Q000378:metabolism; D011554:Pseudopodia / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D000097794:Kalinin
D015815:Cell Adhesion Molecules; D015688:Oncogene Protein pp60(v-src); D051057:Proto-Oncogene Proteins c-akt; D011994:Recombinant Proteins; D000097794:Kalinin
FEBS Lett. 1997 Nov 3;417(1):65-70
9395076
Cancer Sci. 2006 Feb;97(2):91-8
16441418
Proc Natl Acad Sci U S A. 1993 Dec 15;90(24):11767-71
8265624
Biochem J. 2002 Feb 15;362(Pt 1):213-21
11829758
FASEB J. 2008 May;22(5):1530-9
18073332
J Biol Chem. 1992 Sep 5;267(25):17900-6
1517226
J Biol Chem. 1998 Jun 19;273(25):15854-9
9624186
J Cell Biol. 1996 Mar;132(6):1189-98
8601594
Mol Cell Biol. 2002 Feb;22(4):1194-202
11809810
J Biol Chem. 1994 Sep 9;269(36):22779-87
8077230
J Biochem. 1996 Dec;120(6):1196-202
9010770
J Mol Histol. 2008 Aug;39(4):435-46
18670895
Dev Dyn. 2000 Jun;218(2):213-34
10842354
Hum Mol Genet. 1995 May;4(5):959-62
7633458
Exp Cell Res. 2004 Jan 1;292(1):67-77
14720507
Exp Cell Res. 1996 Nov 1;228(2):262-70
8912719
Cancer Res. 2001 Jan 1;61(1):339-47
11196184
Eur J Biochem. 1997 Jun 15;246(3):727-35
9219532
J Biol Chem. 1997 Aug 15;272(33):20502-7
9252362
J Biol Chem. 2002 Dec 20;277(51):49287-95
12379663
J Biol Chem. 2002 Apr 12;277(15):12741-8
11821406
Dev Biol. 1997 Sep 15;189(2):285-300
9299121
Curr Biol. 2009 Feb 10;19(3):260-5
19185493
Mol Biol Cell. 2002 Jul;13(7):2474-85
12134084
FASEB J. 2004 Mar;18(3):457-68
15003991
Biochemistry (Mosc). 2000 Jan;65(1):59-67
10702641
J Biol Chem. 2000 Jul 21;275(29):22495-502
10801807
J Biol Chem. 1995 Sep 15;270(37):21820-6
7665604
Cell. 1989 Sep 8;58(5):933-43
2528412
J Biochem. 2002 Oct;132(4):607-12
12359076
J Biol Chem. 2004 Jun 4;279(23):24774-84
15044476
Nature. 2003 Jul 24;424(6947):452-6
12879075
Matrix Biol. 2005 Aug;24(5):326-32
15979864
Physiol Rev. 2005 Jul;85(3):979-1000
15987800