title: Dishevelled, a Wnt signalling component, is involved in mitotic progression in cooperation with Plk1.
abstract: Wnt signalling is known to promote G1/S progression through the stimulation of gene expression, but whether this signalling regulates mitotic progression is not clear. Here, the function of dishevelled 2 (Dvl2), which transmits the Wnt signal, in mitosis was examined. Dvl2 localized to the spindles and spindle poles during mitosis. When cells were treated with nocodazole, Dvl2 was observed at the kinetochores (KTs). Dvl2 bound to and was phosphorylated at Thr206 by a mitotic kinase, Polo-like kinase 1 (Plk1), and this phosphorylation was required for spindle orientation and stable microtubule (MT)-KT attachment. Dvl2 was also found to be involved in the activation of a spindle assembly checkpoint (SAC) kinase, Mps1, and the recruitment of other SAC components, Bub1 and BubR1, to the KTs. However, the phosphorylation of Dvl2 by Plk1 was dispensable for SAC. Furthermore, Wnt receptors were involved in spindle orientation, but not in MT-KT attachment or SAC. These results suggested that Dvl2 is involved in mitotic progression by regulating the dynamics of MT plus-ends and the SAC in Plk1-dependent and -independent manners.
authors.affiliation: Department of Molecular Biology and Biochemistry, Graduate School of Medicine, Osaka University, Suita, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Kikuchi
authors.forename: Yohei
authors.initials: Y
authors.lastname: Niikura
authors.forename: Katsumi
authors.initials: K
authors.lastname: Kitagawa
authors.forename: Akira
authors.initials: A
authors.lastname: Kikuchi
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001324:Autoantigens / Q000235:genetics / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D000076247:Centromere Protein A; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics / Q000378:metabolism; D000072261:Dishevelled Proteins; D051157:Frizzled Receptors / Q000235:genetics / Q000378:metabolism; D006801:Humans; D018386:Kinetochores / Q000378:metabolism; D060488:Low Density Lipoprotein Receptor-Related Protein-6; D008938:Mitosis / Q000502:physiology*; D015739:Nocodazole / Q000378:metabolism; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000378:metabolism; D011973:Receptors, LDL / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology*; D008941:Spindle Apparatus / Q000378:metabolism; D050257:Tubulin Modulators / Q000378:metabolism; D051153:Wnt Proteins / Q000235:genetics / Q000378:metabolism*; D000097869:Polo-Like Kinase 1
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D001324:Autoantigens; D018797:Cell Cycle Proteins; D000076247:Centromere Protein A; D002868:Chromosomal Proteins, Non-Histone; D000072261:Dishevelled Proteins; D051157:Frizzled Receptors; D060488:Low Density Lipoprotein Receptor-Related Protein-6; D015739:Nocodazole; D010750:Phosphoproteins; D017346:Protein Serine-Threonine Kinases; D011518:Proto-Oncogene Proteins; D034741:RNA, Small Interfering; D043562:Receptors, G-Protein-Coupled; D011973:Receptors, LDL; D011993:Recombinant Fusion Proteins; D050257:Tubulin Modulators; D051153:Wnt Proteins; D000097869:Polo-Like Kinase 1; C000606581:DVL2 protein, human; C489742:FZD2 protein, human; C555475:LRP6 protein, human
references.citation: Dev Cell. 2004 Dec;7(6):831-41
references.pmid: 15572126
references.citation: Chromosoma. 2006 Aug;115(4):320-9
references.pmid: 16601978
references.citation: J Biol Chem. 2008 May 9;283(19):12981-91
references.pmid: 18316369
references.citation: Dev Cell. 2008 Jul;15(1):37-48
references.pmid: 18606139
references.citation: Cell. 2008 Jan 25;132(2):233-46
references.pmid: 18243099
references.citation: Annu Rev Cell Dev Biol. 2004;20:781-810
references.pmid: 15473860
references.citation: Dev Cell. 2007 Nov;13(5):663-676
references.pmid: 17981135
references.citation: Curr Opin Cell Biol. 2008 Apr;20(2):186-93
references.pmid: 18359618
references.citation: Cell. 2003 Feb 21;112(4):407-21
references.pmid: 12600307
references.citation: J Cell Biol. 1996 Dec;135(6 Pt 2):1701-13
references.pmid: 8991084
references.citation: Proc Natl Acad Sci U S A. 2006 Jul 11;103(28):10747-52
references.pmid: 16815967
references.citation: Mol Biol Cell. 2007 Oct;18(10):4024-36
references.pmid: 17671160
references.citation: Genes Dev. 2007 Sep 1;21(17):2205-19
references.pmid: 17785528
references.citation: Cell. 1997 May 30;89(5):727-35
references.pmid: 9182760
references.citation: Dev Cell. 2009 Dec;17(6):788-99
references.pmid: 20059949
references.citation: Trends Cell Biol. 2009 Mar;19(3):119-29
references.pmid: 19208479
references.citation: J Biol Chem. 2009 Oct 9;284(41):28367-28381
references.pmid: 19696028
references.citation: Dev Cell. 2009 Jul;17(1):9-26
references.pmid: 19619488
references.citation: PLoS One. 2008 Jun 11;3(6):e2415
references.pmid: 18545697
references.citation: Mol Biol Cell. 2003 Apr;14(4):1638-51
references.pmid: 12686615
references.citation: Curr Opin Cell Biol. 2005 Feb;17(1):47-54
references.pmid: 15661518
references.citation: Chromosoma. 1998 Dec;107(6-7):424-9
references.pmid: 9914374
references.citation: Nature. 2005 Dec 8;438(7069):867-72
references.pmid: 16341016
references.citation: EMBO J. 2010 Jan 6;29(1):41-54
references.pmid: 19910923
references.citation: Nat Cell Biol. 2006 Oct;8(10):1095-101
references.pmid: 16980960
references.citation: Nat Cell Biol. 2009 Aug;11(8):994-1001
references.pmid: 19620968
references.citation: J Cell Sci. 2010 Feb 1;123(Pt 3):360-8
references.pmid: 20053636
references.citation: J Cell Biol. 2004 Jan 19;164(2):243-53
references.pmid: 14734535
references.citation: Curr Biol. 2006 Apr 18;16(8):731-42
references.pmid: 16631580
references.citation: Nucleic Acids Res. 2005 Jul 1;33(Web Server issue):W184-7
references.pmid: 15980451
references.citation: Curr Biol. 2004 Oct 5;14(19):1712-22
references.pmid: 15458642
references.citation: Nature. 2004 Apr 15;428(6984):767-71
references.pmid: 15085137
references.citation: EMBO J. 2010 Apr 7;29(7):1192-204
references.pmid: 20224554
references.citation: Proc Natl Acad Sci U S A. 2007 Dec 18;104(51):20232-7
references.pmid: 18083840
references.citation: EMBO J. 2005 Apr 20;24(8):1621-33
references.pmid: 15933723
references.citation: Cancer Res. 2006 Nov 1;66(21):10439-48
references.pmid: 17079465
references.citation: J Biol Chem. 2007 May 18;282(20):15217-27
references.pmid: 17376779
references.citation: J Neurosci. 2008 Aug 20;28(34):8644-54
references.pmid: 18716223
references.citation: Nature. 1999 Apr 1;398(6726):422-6
references.pmid: 10201372
references.citation: Mol Biol Cell. 2004 Oct;15(10):4584-96
references.pmid: 15269280
references.citation: J Cell Biol. 2009 Feb 9;184(3):383-90
references.pmid: 19188492
references.citation: Mol Cell. 2007 Jul 6;27(1):3-16
references.pmid: 17612486
references.citation: Trends Cell Biol. 2008 Nov;18(11):536-44
references.pmid: 18848778
references.citation: Proc Natl Acad Sci U S A. 2004 Feb 10;101(6):1584-8
references.pmid: 14747658
references.citation: Dev Biol. 2003 Jan 1;253(1):1-17
references.pmid: 12490194
references.citation: Genes Dev. 2008 Jan 1;22(1):91-105
references.pmid: 18086858
references.citation: Cell. 2001 Jul 13;106(1):83-93
references.pmid: 11461704
references.citation: Oncogene. 2008 Jul 24;27(32):4478-87
references.pmid: 18391981
references.citation: EMBO J. 2002 Apr 2;21(7):1723-32
references.pmid: 11927556
references.citation: Dev Cell. 2003 Jul;5(1):113-25
references.pmid: 12852856
references.citation: Oncogene. 2006 Dec 4;25(57):7469-81
references.pmid: 17143291
references.citation: Proc Natl Acad Sci U S A. 2008 Aug 5;105(31):10762-7
references.pmid: 18669648
references.citation: Science. 2003 Feb 21;299(5610):1228-31
references.pmid: 12595692
references.citation: EMBO J. 2007 Mar 21;26(6):1487-98
references.pmid: 17318179
references.citation: Nat Cell Biol. 2001 Apr;3(4):429-32
references.pmid: 11283619
references.citation: Curr Biol. 2007 Feb 20;17(4):304-15
references.pmid: 17291761
references.citation: J Cell Sci. 2003 Jul 1;116(Pt 13):2627-34
references.pmid: 12775774
references.citation: Dev Cell. 2008 May;14(5):646-59
references.pmid: 18477449
references.citation: Nat Rev Mol Cell Biol. 2005 Dec;6(12):929-42
references.pmid: 16341079
references.citation: Trends Cell Biol. 2000 Jul;10(7):261-7
references.pmid: 10856928
references.citation: Nat Cell Biol. 2005 Jan;7(1):93-8
references.pmid: 15592459
references.citation: Mol Cell. 2004 Aug 13;15(3):317-27
references.pmid: 15304213
references.citation: Nat Rev Mol Cell Biol. 2007 May;8(5):379-93
references.pmid: 17426725
references.citation: Cell Signal. 2008 Feb;20(2):443-52
references.pmid: 18093802
references.citation: J Biol Chem. 2004 Aug 27;279(35):36841-54
references.pmid: 15210710
references.citation: Oncogene. 2006 Jul 13;25(30):4133-46
references.pmid: 16501598
references.citation: EMBO J. 2006 Jun 21;25(12):2814-27
references.pmid: 16763565