title: A doubly signal-amplified DNA detection method based on pre-complexed [Ru(bpy)3]2+-doped silica nanoparticles.
abstract: Easy detection: The target DNA in a 10-100 aM range can be detected by pre-complexed nanoparticles without additional amplification or target labeling. The [Ru(bpy)(3)](2+)-doped silica nanoparticles are hybridized to form a complex with highly enhanced sensitivity (see scheme). This method will be a significant improvement over conventional microarray/fluorescence readout systems.
authors.affiliation: Department of Chemistry, Seoul National University, Seoul 151-747, Korea.
authors.forename: Se Won
authors.initials: SW
authors.lastname: Bae
authors.forename: Min Sun
authors.initials: MS
authors.lastname: Cho
authors.forename: Seong-Suk
authors.initials: SS
authors.lastname: Hur
authors.forename: Chi-Bom
authors.initials: CB
authors.lastname: Chae
authors.forename: Doo Soo
authors.initials: DS
authors.lastname: Chung
authors.forename: Woon-Seok
authors.initials: WS
authors.lastname: Yeo
authors.forename: Jong-In
authors.initials: JI
authors.lastname: Hong
mesh_terms: D004247:DNA / Q000032:analysis*; D015342:DNA Probes / Q000737:chemistry; D061925:Immobilized Nucleic Acids / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry*; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D009942:Organometallic Compounds / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D015342:DNA Probes; D061925:Immobilized Nucleic Acids; D009942:Organometallic Compounds; C415768:tris(2,2'-bipyridyl)ruthenium(II); D012822:Silicon Dioxide; D004247:DNA