Identification and characterization of two novel primate-specific histone H3 variants, H3.X and H3.Y.
Nucleosomal incorporation of specialized histone variants is an important mechanism to generate different functional chromatin states. Here, we describe the identification and characterization of two novel primate-specific histone H3 variants, H3.X and H3.Y. Their messenger RNAs are found in certain human cell lines, in addition to several normal and malignant human tissues. In keeping with their primate specificity, H3.X and H3.Y are detected in different brain regions. Transgenic H3.X and H3.Y proteins are stably incorporated into chromatin in a similar fashion to the known H3 variants. Importantly, we demonstrate biochemically and by mass spectrometry that endogenous H3.Y protein exists in vivo, and that stress stimuli, such as starvation and cellular density, increase the abundance of H3.Y-expressing cells. Global transcriptome analysis revealed that knockdown of H3.Y affects cell growth and leads to changes in the expression of many genes involved in cell cycle control. Thus, H3.Y is a novel histone variant involved in the regulation of cellular responses to outside stimuli.
Wiedemann
Sonja M
SM
Adolf-Butenandt-Institute, Department of Molecular Biology, Ludwig Maximilians University of Munich, 80336 Munich, Germany.
Mildner
Silke N
SN
Bönisch
Clemens
C
Israel
Lars
L
Maiser
Andreas
A
Matheisl
Sarah
S
Straub
Tobias
T
Merkl
Rainer
R
Leonhardt
Heinrich
H
Kremmer
Elisabeth
E
Schermelleh
Lothar
L
Hake
Sandra B
SB
D000818:Animals; D002453:Cell Cycle / Q000235:genetics / Q000502:physiology; D045744:Cell Line, Tumor; D002843:Chromatin; D004926:Escherichia coli / Q000235:genetics; D014644:Genetic Variation; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006367:HeLa Cells; D006657:Histones / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D013058:Mass Spectrometry; D051379:Mice; D041681:NIH 3T3 Cells; D009447:Neuroblastoma / Q000473:pathology; D009707:Nucleosomes; D011323:Primates / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D014162:Transfection
D002843:Chromatin; D006657:Histones; D009707:Nucleosomes; D012333:RNA, Messenger; D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins
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