Antihypertensive effect of insect cells: in vitro and in vivo evaluation.
In this study, we investigated the in vitro ACE inhibitory and in vivo antihypertensive effect of insect cell extracts. The IC(50) of three insect cell lines from different type and insect species origin: S2 (embryo, Drosophila melanogaster), Sf21 (ovary, Spodoptera frugiperda) and Bm5 (ovary, Bombyx mori), were evaluated. Most interesting results were that the IC(50) values ranged between 0.4 and 0.9 mg/ml, and that an extra hydrolysis with gastrointestinal enzymes did not increase the ACE inhibitory activity conspicuously. Finally, a single oral administration with a gavage of 150 mg cell extract/kg BW to spontaneous hypertensive rats (SHR) significantly decreased (p<0.05) their systolic blood pressure (SBP) with 5-6% (9-12 mmHg) compared to the controls at 6 h post-administration. Here the undigested and digested insect S2 cell extracts were equal in activity to lower the SBP. To the best of our knowledge, this is the first report of in vivo antihypertensive activity of insect cell extracts and this without an extra digestion requirement.
Staljanssens
Dorien
D
Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, B-9000 Ghent, Belgium.
Van Camp
John
J
Herregods
Griet
G
Dhaenens
Maarten
M
Deforce
Dieter
D
Van de Voorde
Johan
J
Smagghe
Guy
G
D000818:Animals; D000959:Antihypertensive Agents / Q000737:chemistry / Q000627:therapeutic use*; D001794:Blood Pressure; D012831:Bombyx / Q000737:chemistry; D002460:Cell Line; D002850:Chromatography, Gel; D004331:Drosophila melanogaster / Q000737:chemistry; D005260:Female; D006973:Hypertension / Q000188:drug therapy*; D020128:Inhibitory Concentration 50; D008297:Male; D051379:Mice; D051381:Rats; D011918:Rats, Inbred SHR; D018411:Spodoptera / Q000737:chemistry
D000959:Antihypertensive Agents