Biodegradation of PAHs in soil: Influence of chemical structure, concentration and multiple amendment.
The influence of PAH chemical structure and concentration, added in either single (75 or 300 mg kg(-1)) or multiple (2 × 75, 2 × 150 or 4 × 75 mg kg(-1)) applications as single- or multiple-contaminant systems, on the development of PAH biodegradation in a pristine soil was investigated. Development in microbial catabolic ability was assessed at 0, 28, 56 and 84 d by monitoring (14)C-naphthalene, (14)C-phenanthrene and (14)C-pyrene mineralisation over 14 d in respirometric assays. The presence of other contaminants influenced the ability of the indigenous microflora to mineralise structurally different contaminants over time. (14)C-Naphthalene mineralisation was inhibited by the presence of other contaminants; whereas the presence of naphthalene significantly enhanced rates of mineralisation in multiple-contaminant systems containing (14)C-phenanthrene and (14)C-pyrene. Generally, increasing the number of contaminant applications has implications for catabolic activity of soil microbes. It is suggested the toxic nature of PAHs retarded mineralisation at increased contaminant concentrations.
Couling
Natalie R
NR
Lancaster Environment Centre, Lancaster University, United Kingdom.
Towell
Marcie G
MG
Semple
Kirk T
KT
D001673:Biodegradation, Environmental; D008903:Minerals / Q000737:chemistry; D009281:Naphthalenes / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D010616:Phenanthrenes / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*
D008903:Minerals; D009281:Naphthalenes; D010616:Phenanthrenes; D011084:Polycyclic Aromatic Hydrocarbons; D012989:Soil Pollutants; C031721:naphthalene; C031181:phenanthrene