title: Comparison of hypercrosslinked polystyrene columns for the separation of nitrogen group-types in petroleum using High Performance Liquid Chromatography.
abstract: High performance liquid chromatography in a quasi-normal phase mode (QNP) is used to separate the nitrogen group-types (pyrrole and pyridine) that are found in petroleum. A new type of stationary phase, hypercrosslinked polystyrene, is used to achieve this separation. Three different hypercrosslinked polystyrene stationary phases are compared under quasi-normal phase mode; a commercial 5-HGN packing, and two hypercrosslinked phases on silica particles. The utility of the columns for petroleum-based separations was explored with the use of 21 analytical standards. Partial elucidation of adsorption retention mechanisms for the columns are shown, as well as a comparison of retention characteristics for the three columns. The silica particle column derived with toluene (HC-Tol) was found to have the best selectivity for nitrogen group-types and polycyclic aromatic hydrocarbons (PAHs), attaining a separation under gradient conditions in less than 30 min.
authors.affiliation: Department of Chemistry, University of Alberta, Gunning/Lemieux Chemistry Centre, Edmonton, Alberta T6G 2G2, Canada.
authors.forename: Nicole E
authors.initials: NE
authors.lastname: Oro
authors.forename: Charles A
authors.initials: CA
authors.lastname: Lucy
mesh_terms: D000327:Adsorption; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D016014:Linear Models; D009584:Nitrogen / Q000737:chemistry*; D010578:Petroleum / Q000032:analysis*; D011084:Polycyclic Aromatic Hydrocarbons / Q000737:chemistry / Q000302:isolation & purification; D011137:Polystyrenes / Q000737:chemistry*; D011725:Pyridines / Q000737:chemistry / Q000302:isolation & purification*; D011758:Pyrroles / Q000737:chemistry / Q000302:isolation & purification*; D012822:Silicon Dioxide / Q000737:chemistry
chemical_list: D010578:Petroleum; D011084:Polycyclic Aromatic Hydrocarbons; D011137:Polystyrenes; D011725:Pyridines; D011758:Pyrroles; D012822:Silicon Dioxide; D009584:Nitrogen