title: Localization of butyrylcholinesterase at the neuromuscular junction of normal and acetylcholinesterase knockout mice.
abstract: At the mouse neuromuscular junction (NMJ), there are two distinct cholinesterases (ChE): acetylcholinesterase (AChE) and butyrylcholinesterase (BChE). Until now, it has been difficult to determine the precise localization of BChE at the NMJ. In this study, we use a modification of Koelle's method to stain AChE and BChE activity. This method does not interfere with fluorescent co-staining, which allows precise co-localization of ChE and other synaptic molecules at the NMJ. We demonstrate that AChE and BChE exhibit different localization patterns at the mouse NMJ. AChE activity is present both in the primary cleft and in the secondary folds, whereas BChE activity appears to be almost absent in the primary cleft and to be concentrated in subsynaptic folds. The same localization for BChE is observed in the AChE-knockout (KO) mouse NMJ. Collagenase treatment removed AChE from the primary cleft, but not from secondary folds in the wild-type mouse, whereas in the AChE-KO mouse, BChE remains in the secondary folds. After peripheral nerve injury and regeneration, BChE localization is not modified in either normal or KO mice. In conclusion, specific localization of BChE in the secondary folds of the NMJ suggests that this enzyme is not a strict surrogate of AChE and that the two enzymes have two different roles.
authors.affiliation: Laboratoire CRRET, Université Paris-Est, EAC CNRS 7149, 61 avenue du Général de Gaulle, 94010 Créteil, France. blondet@univ-paris12.fr
authors.forename: Brigitte
authors.initials: B
authors.lastname: Blondet
authors.forename: Gilles
authors.initials: G
authors.lastname: Carpentier
authors.forename: Arnaud
authors.initials: A
authors.lastname: Ferry
authors.forename: Arnaud
authors.initials: A
authors.lastname: Chatonnet
authors.forename: José
authors.initials: J
authors.lastname: Courty
mesh_terms: D000110:Acetylcholinesterase / Q000172:deficiency* / Q000235:genetics; D000818:Animals; D002091:Butyrylcholinesterase / Q000032:analysis* / Q000378:metabolism; D005260:Female; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D009469:Neuromuscular Junction / Q000201:enzymology*; D010525:Peripheral Nerves / Q000378:metabolism / Q000473:pathology
chemical_list: D000110:Acetylcholinesterase; D002091:Butyrylcholinesterase
references.citation: J Neurosci Res. 1985;14(1):95-103
references.pmid: 4020901
references.citation: Neurosci Res. 2006 Aug;55(4):389-96
references.pmid: 16766072
references.citation: Trends Pharmacol Sci. 1989 Aug;10(8):325-9
references.pmid: 2686130
references.citation: Mol Biol Cell. 2007 Aug;18(8):2904-11
references.pmid: 17538015
references.citation: J Pharmacol Exp Ther. 2000 Jun;293(3):896-902
references.pmid: 10869390
references.citation: Biochim Biophys Acta. 2007 Jun;1770(6):919-26
references.pmid: 17368730
references.citation: Neurochem Res. 2006 Jul;31(7):907-13
references.pmid: 16804756
references.citation: J Histochem Cytochem. 1954 Mar;2(2):115-21
references.pmid: 13143194
references.citation: Brain Res Brain Res Rev. 2004 Dec;47(1-3):96-104
references.pmid: 15572165
references.citation: Brain Res. 2002 Mar 15;930(1-2):53-7
references.pmid: 11879795
references.citation: C R Hebd Seances Acad Sci. 1952 Aug 11;235(6):434-6
references.pmid: 13009934
references.citation: J Cell Biol. 1969 Jul;42(1):122-34
references.pmid: 5786978
references.citation: Toxicology. 2007 Apr 20;233(1-3):60-9
references.pmid: 17194517
references.citation: Brain Res Dev Brain Res. 2002 Jul 30;137(1):43-54
references.pmid: 12128253
references.citation: Eur J Neurosci. 2003 Sep;18(6):1419-27
references.pmid: 14511322
references.citation: J Biol Chem. 1983 Jul 25;258(14):8794-8
references.pmid: 6863310
references.citation: Annu Rev Neurosci. 1982;5:57-106
references.pmid: 6176173
references.citation: Curr Opin Pharmacol. 2009 Jun;9(3):316-25
references.pmid: 19423392
references.citation: J Biol Chem. 2003 Apr 11;278(15):12601-4
references.pmid: 12556454
references.citation: J Cell Biol. 1979 Nov;83(2 Pt 1):357-70
references.pmid: 91619
references.citation: Dev Biol. 1986 Sep;117(1):13-23
references.pmid: 3743893
references.citation: Muscle Nerve. 2004 Sep;30(3):317-27
references.pmid: 15318343
references.citation: J Neurocytol. 2003 Jun-Sep;32(5-8):883-903
references.pmid: 15034274
references.citation: Br J Pharmacol. 2003 Jan;138(1):177-87
references.pmid: 12522088
references.citation: J Cell Biol. 1994 Apr;125(1):183-96
references.pmid: 8138570
references.citation: J Physiol Paris. 1998 Jun-Aug;92(3-4):195-8
references.pmid: 9789807
references.citation: J Cell Biol. 1999 Mar 22;144(6):1349-60
references.pmid: 10087275
references.citation: Nature. 1978 Jan 12;271(5641):172-4
references.pmid: 622157
references.citation: Chem Biol Interact. 2008 Sep 25;175(1-3):26-9
references.pmid: 18599029
references.citation: J Neurosci Methods. 1998 Jun 1;81(1-2):63-71
references.pmid: 9696311
references.citation: Exp Brain Res. 1999 Apr;125(4):426-34
references.pmid: 10323288
references.citation: J Neurochem. 2000 Sep;75(3):1320-31
references.pmid: 10936216
references.citation: Neurochem Int. 1991;18(3):425-33
references.pmid: 20504721
references.citation: Proc Soc Exp Biol Med. 1949 Apr;70(4):617-22
references.pmid: 18149447
references.citation: Life Sci. 2007 May 30;80(24-25):2380-5
references.pmid: 17467011