title: Increased intramuscular nerve branching and inhibition of programmed cell death of chick embryo motoneurons by immunoglobulins from patients with motoneuron disease.
abstract: Massive programmed cell death (PCD) of developing chick embryo motoneurons (MNs) occurs in a well defined temporal and spatial sequence between embryonic day (E) 6 and E10. We have found that, when administered in ovo, either circulating immunoglobulins G (IgGs) or cerebrospinal fluid from patients with MN disease can rescue a significant number of chick embryo MNs from normally occurring PCD. An increase of branching of intramuscular nerves was also observed that may account for the rescuing effects of pathologic IgGs. Proteomic analysis and further analysis by ELISA indicated that these effects may be mediated by the interaction of circulating human immunoglobulins with proteins of the semaphorin family.
authors.affiliation: Universitat de Lleida, Facultat de Medicina, Departament de Medicina Experimental, Lleida, Catalonia, Spain.
authors.forename: Sara
authors.initials: S
authors.lastname: Hernández
authors.forename: Laura
authors.initials: L
authors.lastname: Texidó
authors.forename: Jordi
authors.initials: J
authors.lastname: Calderó
authors.forename: Dolors
authors.initials: D
authors.lastname: Ciutat
authors.forename: Lídia
authors.initials: L
authors.lastname: Piedrafita
authors.forename: Anna
authors.initials: A
authors.lastname: Casanovas
authors.forename: Joan
authors.initials: J
authors.lastname: Blasi
authors.forename: Carles
authors.initials: C
authors.lastname: Solsona
authors.forename: Mònica
authors.initials: M
authors.lastname: Povedano
authors.forename: Ricardo
authors.initials: R
authors.lastname: Rojas
authors.forename: Isabel
authors.initials: I
authors.lastname: Illa
authors.forename: James
authors.initials: J
authors.lastname: Caress
authors.forename: David
authors.initials: D
authors.lastname: Prevette
authors.forename: Ronald W
authors.initials: RW
authors.lastname: Oppenheim
authors.forename: Carol
authors.initials: C
authors.lastname: Milligan
authors.forename: Josep E
authors.initials: JE
authors.lastname: Esquerda
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002642:Chick Embryo; D002522:Chlorocebus aethiops; D004305:Dose-Response Relationship, Drug; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D005727:Ganglia, Spinal / Q000166:cytology; D020439:Growth Cones / Q000187:drug effects; D006801:Humans; D007136:Immunoglobulins / Q000276:immunology / Q000494:pharmacology*; D066298:In Vitro Techniques; D008297:Male; D016472:Motor Neuron Disease / Q000097:blood / Q000276:immunology*; D009046:Motor Neurons / Q000166:cytology / Q000187:drug effects*; D018482:Muscle, Skeletal / Q000196:embryology / Q000294:innervation*; D009469:Neuromuscular Junction / Q000187:drug effects* / Q000502:physiology; D040901:Proteomics / Q000379:methods; D039961:Semaphorins / Q000378:metabolism; D044967:Serum / Q000737:chemistry / Q000276:immunology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D013223:Statistics as Topic; D018709:Statistics, Nonparametric; D014162:Transfection / Q000379:methods; D014404:Tubulin / Q000378:metabolism
chemical_list: D007136:Immunoglobulins; D039961:Semaphorins; D014404:Tubulin