A preliminary study on the activation and antigen presentation of hepatitis B virus core protein virus-like particle-pulsed bone marrow-derived dendritic cells.
Hepatitis B virus core protein virus-like particles (HBc-VLPs) act as a strong immunogen and are suitable for uptake by dendritic cells (DCs), in which they directly promote DC maturation and migration. To illustrate the utility of global proteomic analysis techniques in elucidating the molecular events that are altered in HBc-VLP-pulsed bone marrow-derived DCs (BMDCs) and to gain a better understanding of the molecular mechanisms of capture and processing of HBc-VLP-pulsed BMDCs, an antigen (Ag) delivery system based on HBc-VLP-pulsed BMDCs was developed. Two-dimensional electrophoresis (2-DE) and tandem mass spectrometry (MS/MS) analyses were utilized to analyze the differential protein expression patterns between HBc-VLP-pulsed and untreated BMDCs. Protein spots with significantly altered expression levels were detected, identified and validated. The results showed that exogenous HBc-VLPs were phagocytosed efficiently by BMDCs and enhanced the efficacy of BMDC maturation and Ag presentation, VLPs also induced high levels of Ag-specific CD8(+) T cells that displayed high cytotoxic T lymphocyte (CTL) activity in vivo. Several differentially expressed proteins, including growth factor receptor bound protein 2 (Grb2) and annexin A2 (AnxA2), were detected by proteomic analysis, identified by mass spectrometry and validated by western blot.
Ding
Fei-Xiang
FX
Department of Medical Genetics, The Second Military Medical University, Shanghai 200433, P R China. wfsjz70@hotmail.com
Xian
Xian
X
Guo
Ying-Jun
YJ
Liu
Yin
Y
Wang
Yue
Y
Yang
Fu
F
Wang
Yu-Zhao
YZ
Song
Shu-Xia
SX
Wang
Fang
F
Sun
Shu-Han
SH
D000818:Animals; D017951:Antigen Presentation / Q000276:immunology*; D015153:Blotting, Western; D001854:Bone Marrow Cells / Q000166:cytology*; D016207:Cytokines / Q000378:metabolism; D003602:Cytotoxicity, Immunologic; D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology*; D015180:Electrophoresis, Gel, Two-Dimensional; D000939:Epitopes; D006515:Hepatitis B virus / Q000276:immunology*; D013058:Mass Spectrometry; D051379:Mice; D008810:Mice, Inbred C57BL; D010587:Phagocytosis; D010641:Phenotype; D040901:Proteomics; D014758:Viral Core Proteins / Q000276:immunology*; D014771:Virion / Q000276:immunology*
D016207:Cytokines; D000939:Epitopes; D014758:Viral Core Proteins