Development of SCAR markers and PCR assays for single or simultaneous species-specific detection of Phytophthora nicotianae and Pythium helicoides in ebb-and-flow irrigated kalanchoe.
Phytophthora nicotianae and Pythium helicoides are important water-borne oomycete pathogens of irrigated ornamentals particularly ebb-and-flow irrigated kalanchoe in Japan. We developed novel PCR-based sequence characterized amplified region markers and assays for rapid identification and species-specific detection of both pathogens in separate PCR reactions or simultaneously in a duplex PCR.
Ahonsi
Monday O
MO
River Basin Research Center, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan. mahonsi@igb.uiuc.edu
Ling
Yin
Y
Kageyama
Koji
K
D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D007564:Japan; D031962:Kalanchoe / Q000382:microbiology*; D008969:Molecular Sequence Data; D009172:Mycology / Q000379:methods*; D010838:Phytophthora / Q000235:genetics / Q000302:isolation & purification*; D010935:Plant Diseases / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011775:Pythium / Q000235:genetics / Q000302:isolation & purification*; D017422:Sequence Analysis, DNA
D004271:DNA, Fungal