Cardiotoxic effects of Loxosceles intermedia spider venom and the recombinant venom toxin rLiD1.
Loxosceles spider bites cause many human injuries worldwide. Injections in mice of whole Loxosceles (L.) intermedia venom or a recombinant toxin (rLiD1) produce systemic symptoms similar to those detected in envenomed humans. This animal model was used to characterize the effects of Loxosceles intermedia venom in cardiac tissues. L. intermedia antigens were detected by ELISA in kidney, heart, lung and liver of experimentally envenomed mice. In addition, rLiD1 binding to cardiomyocytes was demonstrated by immunofluorescence and confocal microscopy. Furthermore, isolated perfused heart preparations and ventricular cardiomyocytes from envenomed mice showed heart function impairment, and a significant increase of I(Ca,L) density and intracellular Ca(2+) transients, respectively. Thus, L. intermedia spider venom, as shown through the use of the recombinant toxin rLiD1, causes cardiotoxic effects and a protein from the sphingomyelinase D family plays a key role in heart dysfunction. Thus, L. intermedia spider venom and the Loxtox rLiD1 play a key role in heart dysfunction.
Dias-Lopes
Camila
C
Biochemistry Departament, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, CP: 486, CEP: 30161-970, Belo Horizonte, Brazil.
Felicori
Liza
L
Guimarães
Gabriela
G
Gomes
Eneas R M
ER
Roman-Campos
Danilo
D
Duarte
Hugo
H
Damasceno
Denis
D
Martins
Marilia
M
Kalapothakis
Evanguedes
E
Almeida
Alvair P
AP
Granier
Claude
C
Cruz
Jader S
JS
Guatimosim
Silvia
S
Chávez-Olórtegui
Carlos
C
D000818:Animals; D000941:Antigens / Q000032:analysis; D002118:Calcium / Q000378:metabolism; D054715:Cardiotoxins / Q000276:immunology / Q000302:isolation & purification / Q000633:toxicity*; D002478:Cells, Cultured; D003402:Creatine Kinase / Q000097:blood; D052279:Creatine Kinase, MB Form / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006321:Heart / Q000187:drug effects*; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009206:Myocardium / Q000473:pathology*; D032383:Myocytes, Cardiac / Q000187:drug effects; D018408:Patch-Clamp Techniques; D010727:Phosphoric Diester Hydrolases / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology / Q000633:toxicity*; D011993:Recombinant Fusion Proteins; D013111:Spider Venoms / Q000276:immunology / Q000302:isolation & purification / Q000633:toxicity*
D000941:Antigens; D054715:Cardiotoxins; D011993:Recombinant Fusion Proteins; D013111:Spider Venoms; C055161:loxosceles venom; D003402:Creatine Kinase; D052279:Creatine Kinase, MB Form; D010727:Phosphoric Diester Hydrolases; C055160:sphingomyelin phosphodiesterase D; D002118:Calcium