A role for pectin de-methylesterification in a developmentally regulated growth acceleration in dark-grown Arabidopsis hypocotyls.
• We focused on a developmentally regulated growth acceleration in the dark-grown Arabidopsis hypocotyl to study the role of changes in cell wall metabolism in the control of cell elongation. • To this end, precise transcriptome analysis on dissected dark-grown hypocotyls, Fourier transform infrared (FT-IR) microspectroscopy and kinematic analysis were used. • Using a cellulose synthesis inhibitor, we showed that the growth acceleration marks a developmental transition during which growth becomes uncoupled from cellulose synthesis. We next investigated the cellular changes that take place during this transition. FT-IR microspectroscopy revealed significant changes in cell wall composition during, but not after, the growth acceleration. Transcriptome analysis suggested a role for cell wall remodeling, in particular pectin modification, in this growth acceleration. This was confirmed by the overexpression of a pectin methylesterase inhibitor, which caused a delay in the growth acceleration. • This study shows that the acceleration of cell elongation marks a developmental transition in dark-grown hypocotyl cells and supports a role for pectin de-methylesterification in the timing of this event.
Pelletier
Sandra
S
Institut Jean-Pierre Bourgin, UMR1318 INRA-AgroParisTech, INRA Centre de Versailles-Grignon, Versailles, France.
Van Orden
Jürgen
J
Wolf
Sebastian
S
Vissenberg
Kris
K
Delacourt
Julien
J
Ndong
Yves Assoumou
YA
Pelloux
Jérôme
J
Bischoff
Volker
V
Urbain
Aurélie
A
Mouille
Grégory
G
Lemonnier
Gaëtan
G
Renou
Jean-Pierre
JP
Höfte
Herman
H
D017360:Arabidopsis / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism; D002265:Carboxylic Ester Hydrolases / Q000037:antagonists & inhibitors*; D002473:Cell Wall / Q000378:metabolism*; D002482:Cellulose / Q000096:biosynthesis; D003624:Darkness; D004951:Esterification; D015870:Gene Expression; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D018546:Hypocotyl / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D010368:Pectins / Q000378:metabolism*; D017550:Spectroscopy, Fourier Transform Infrared
D010368:Pectins; D002482:Cellulose; D002265:Carboxylic Ester Hydrolases; C022323:pectinesterase