A rapid microtiter plate assay for measuring the effect of compounds on Staphylococcus aureus membrane potential.
We developed a homogenous microtiter based assay using the cationic dye 3, 3'-Diethyloxacarbocyanine iodide, DiOC2(3), to measure the effect of compounds on membrane potential in Staphylococcus aureus. In a screen of 372 compounds from a synthetic compound collection with anti-Escherichia coli activity due to unknown modes of action at least 17% demonstrated potent membrane activity, enabling rapid discrimination of nuisance compounds.
Gentry
Daniel R
DR
Antibacterial Discovery Performance Unit, Infectious Diseases Center of Excellence in Drug Discovery, GlaxoSmithKline Pharmaceuticals, Collegeville, PA 19426, United States. dan.r.gentry@gsk.com
Wilding
Imogen
I
Johnson
John M
JM
Chen
Dongzhao
D
Remlinger
Katja
K
Richards
Cindy
C
Neill
Susan
S
Zalacain
Magdalena
M
Rittenhouse
Stephen F
SF
Gwynn
Michael N
MN
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001431:Bacteriological Techniques / Q000379:methods*; D002232:Carbocyanines / Q000378:metabolism; D004926:Escherichia coli / Q000187:drug effects; D057166:High-Throughput Screening Assays / Q000379:methods*; D020128:Inhibitory Concentration 50; D008564:Membrane Potentials / Q000187:drug effects*; D013211:Staphylococcus aureus / Q000187:drug effects*
D000900:Anti-Bacterial Agents; D002232:Carbocyanines; C081554:3,3'-diethyloxacarbocyanine