Elevated c-Src and c-Yes expression in malignant skin cancers.
BACKGROUND
Src family kinases (SFKs) play an important role in cancer proliferation, survival, motility, invasiveness, metastasis, and angiogenesis. Among the SFKs, c-Src and c-Yes are particularly over-expressed or hyper-activated in many human epithelial cancers. However, only a few studies have attempted to define the expression and role of c-Src and c-Yes in cutaneous carcinomas.


OBJECTIVE
To investigate the expression of c-Src and c-Yes in cutaneous carcinomas to include malignant melanoma (MM), squamous cell carcinoma (SCC) and basal cell carcinoma (BCC).


METHODS
We examined 6 normal skin tissues and 18 malignant skin tumor tissues using western blotting for the expression of c-Src and c-Yes. In another set, 16 specimens of MM, 16 SCCs and 16 BCCs were analyzed for the expression of c-Src and c-Yes using immunohistochemical staining.


RESULTS
Western blotting showed that c-Src was expressed in all malignant skin tumors, but not in normal skin, while c-Yes was expressed in MM and SCC, but not in BCC and normal skin. Immunohistochemical staining results of c-Src and c-Yes in MM, SCC, and BCC mirrored those of the western blot analysis.


CONCLUSIONS
c-Src, rather than c-Yes, plays a key role in the proliferation and progression of malignant skin cancers.
Lee
Jang Hyun
JH
Molecular Cancer Research Center, College of Medicine, Soonchunhyang University, Chunan, Korea.
Pyon
Jae-Kyung
JK
Kim
Dong Wook
DW
Lee
Sang Han
SH
Nam
Hae Seon
HS
Kim
Chul Han
CH
Kang
Sang Gue
SG
Lee
Yoon Jin
YJ
Park
Mi Youn
MY
Jeong
Dong Jun
DJ
Cho
Moon Kyun
MK
D000368:Aged; D000369:Aged, 80 and over; D015153:Blotting, Western; D000081247:CSK Tyrosine-Protein Kinase; D002280:Basal Cell Carcinoma / Q000201:enzymology* / Q000473:pathology; D002294:Carcinoma, Squamous Cell / Q000201:enzymology* / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008545:Melanoma / Q000201:enzymology* / Q000473:pathology; D008875:Middle Aged; D011505:Protein-Tyrosine Kinases / Q000032:analysis*; D011518:Proto-Oncogene Proteins / Q000032:analysis*; D051575:Proto-Oncogene Proteins c-yes / Q000032:analysis*; D056910:Republic of Korea; D012878:Skin Neoplasms / Q000201:enzymology* / Q000473:pathology; D019061:src-Family Kinases
D011518:Proto-Oncogene Proteins; D011505:Protein-Tyrosine Kinases; D000081247:CSK Tyrosine-Protein Kinase; D051575:Proto-Oncogene Proteins c-yes; C489834:YES1 protein, human; D019061:src-Family Kinases; C000631346:CSK protein, human
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