title: Autoassembly protein arrays for analyzing antibody cross-reactivity.
abstract: We report an autoassembly protein array capable of rapidly screening for aberrant antibody-antigen binding events. Our technique combines magnetic nanoparticle technology with proximity-based, magnetically responsive nanosensors for rapid (under 15 min) and high-density screening of antibody cross-reactivity at sensitivities down to 50 fM in a homogeneous assay. This method will enable the identification of the precise cause of aberrant or cross-reactive binding events in an easy-to-use, rapid, and high-throughput manner.
authors.affiliation: Department of Bioengineering, School of Medicine, Stanford University, Stanford, California 94305, United States.
authors.forename: Richard S
authors.initials: RS
authors.lastname: Gaster
authors.forename: Drew A
authors.initials: DA
authors.lastname: Hall
authors.forename: Shan X
authors.initials: SX
authors.lastname: Wang
mesh_terms: D000906:Antibodies / Q000032:analysis* / Q000276:immunology*; D000937:Antigen-Antibody Reactions; D003429:Cross Reactions / Q000276:immunology*; D057166:High-Throughput Screening Assays; D007118:Immunoassay; D008280:Magnetics; D036103:Nanotechnology; D040081:Protein Array Analysis
chemical_list: D000906:Antibodies
references.citation: J Virol. 1983 Dec;48(3):709-20
references.pmid: 6313968
references.citation: Nat Genet. 2002 Dec;32 Suppl:526-32
references.pmid: 12454649
references.citation: J Clin Microbiol. 1987 Sep;25(9):1711-6
references.pmid: 3308951
references.citation: Mol Cell Proteomics. 2008 Dec;7(12):2464-74
references.pmid: 18678562
references.citation: Nat Med. 2009 Nov;15(11):1327-32
references.pmid: 19820717
references.citation: J Histochem Cytochem. 1989 Feb;37(2):159-64
references.pmid: 2463300
references.citation: Nat Med. 2004 Dec;10(12):1390-6
references.pmid: 15558056
references.citation: Mod Pathol. 2008 Feb;21(2):186-91
references.pmid: 18084248
references.citation: Science. 1995 Oct 20;270(5235):467-70
references.pmid: 7569999
references.citation: Nat Biotechnol. 1998 Jan;16(1):40-4
references.pmid: 9447591
references.citation: J Autoimmun. 1998 Apr;11(2):163-7
references.pmid: 9650095
references.citation: Hum Antibodies. 2005;14(1-2):7-15
references.pmid: 16424595
references.citation: Biosens Bioelectron. 2010 May 15;25(9):2051-7
references.pmid: 20207130
references.citation: Nat Methods. 2008 Jun;5(6):535-8
references.pmid: 18469824
references.citation: Nat Biotechnol. 2002 Mar;20(3):225-9
references.pmid: 11875416
references.citation: Nat Biotechnol. 2002 Apr;20(4):359-65
references.pmid: 11923841
references.citation: Nature. 2002 Apr 25;416(6883):885-91
references.pmid: 11976691
references.citation: Proc Natl Acad Sci U S A. 2008 Dec 30;105(52):20637-40
references.pmid: 19074273
references.citation: Nat Biotechnol. 2003 Dec;21(12):1509-12
references.pmid: 14608365