Development of novel nanoparticles shelled with heparin for berberine delivery to treat Helicobacter pylori.
Various approaches have been proposed to overcome the unpleasant side-effects associated with antibiotic treatment for Helicobacter pylori. The limited effectiveness of such approaches has forced researchers to consider alternative strategies to eliminate H. pylori infection. The plant alkaloid berberine is known to significantly reduce proliferation of H. pylori. To localize berberine to the site of H. pylori infection, this study developed a novel nanoparticle berberine carrier with a heparin shell. Analysis of a simulated gastrointestinal medium indicated that the proposed in vitro drug carrier system effectively controlled the release of berberine, which interacted specifically with the intercellular space at the site of H. pylori infection. Furthermore, the prepared nanoparticles significantly increased the suppressive effect of berberine on H. pylori growth while efficiently reducing cytotoxic effects in H. pylori-infected cells.
Chang
Chiung-Hung
CH
School of Chinese Medicine, China Medical University and Department of Traditional Chinese Medicine, Taichung Veterans General Hospital, Taichung, Taiwan, ROC.
Huang
Wen-Ying
WY
Lai
Chih-Ho
CH
Hsu
Yuan-Man
YM
Yao
Yi-Hsing
YH
Chen
Ting-Yu
TY
Wu
Jing-Yan
JY
Peng
Shu-Fen
SF
Lin
Yu-Hsin
YH
D001599:Berberine / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D016503:Drug Delivery Systems; D005453:Fluorescence; D016480:Helicobacter pylori / Q000166:cytology / Q000187:drug effects* / Q000254:growth & development; D006493:Heparin / Q000494:pharmacology*; D006801:Humans; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D018613:Microscopy, Confocal; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D012996:Solutions; D021241:Spectrometry, Mass, Electrospray Ionization; D017550:Spectroscopy, Fourier Transform Infrared; D055672:Static Electricity
D012996:Solutions; D001599:Berberine; D006493:Heparin; D048271:Chitosan