title: FBI-1 functions as a novel AR co-repressor in prostate cancer cells.
abstract: The pro-oncogene FBI-1, encoded by Zbtb7a, is a transcriptional repressor that belongs to the POK (POZ/BTB and Krüppel) protein family. In this study, we investigated a potential interaction between androgen receptor (AR) signaling and FBI-1 and demonstrated that overexpression of FBI-1 inhibited ligand-dependent AR activation. A protein-protein interaction was identified between FBI-1 and AR in a ligand-dependent manner. Furthermore, FBI-1, AR and SMRT formed a ternary complex and FBI-1 enhanced the recruitment of NCoR and SMRT to endogenous PSA upstream sequences. Our data also indicated that the FBI-1-mediated inhibition of AR transcriptional activity is partially dependent on HDAC. Interestingly, FBI-1 plays distinct roles in regulating LNCaP (androgen-dependent) and PC-3 cell (androgen-independent) proliferation.
authors.affiliation: Institute of Disease Control and Prevention, Chinese Academy of Military Medical Sciences, Beijing, People's Republic of China.
authors.forename: Jiajun
authors.initials: J
authors.lastname: Cui
authors.forename: Yutao
authors.initials: Y
authors.lastname: Yang
authors.forename: Chuanfu
authors.initials: C
authors.lastname: Zhang
authors.forename: Pinliang
authors.initials: P
authors.lastname: Hu
authors.forename: Wei
authors.initials: W
authors.lastname: Kan
authors.forename: Xianhong
authors.initials: X
authors.lastname: Bai
authors.forename: Xuelin
authors.initials: X
authors.lastname: Liu
authors.forename: Hongbin
authors.initials: H
authors.lastname: Song
mesh_terms: D045744:Cell Line, Tumor; D047369:Chromatin Immunoprecipitation; D017931:DNA Primers / Q000235:genetics; D004268:DNA-Binding Proteins / Q000378:metabolism*; D006655:Histone Deacetylases / Q000378:metabolism; D006801:Humans; D047468:Immunoprecipitation; D008156:Luciferases; D008297:Male; D046912:Multiprotein Complexes / Q000378:metabolism*; D056985:Nuclear Receptor Co-Repressor 2 / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics; D011471:Prostatic Neoplasms / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000235:genetics; D011944:Receptors, Androgen / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000502:physiology*; D014157:Transcription Factors / Q000378:metabolism*; D014162:Transfection
chemical_list: D017931:DNA Primers; D004268:DNA-Binding Proteins; D046912:Multiprotein Complexes; C539130:NCOR2 protein, human; D056985:Nuclear Receptor Co-Repressor 2; D034741:RNA, Small Interfering; D011944:Receptors, Androgen; D014157:Transcription Factors; C485514:ZBTB7A protein, human; D008156:Luciferases; D006655:Histone Deacetylases
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