title: Improving image segmentation performance and quantitative analysis via a computer-aided grading methodology for optical coherence tomography retinal image analysis.
abstract: We demonstrate quantitative analysis and error correction of optical coherence tomography (OCT) retinal images by using a custom-built, computer-aided grading methodology. A total of 60 Stratus OCT (Carl Zeiss Meditec, Dublin, California) B-scans collected from ten normal healthy eyes are analyzed by two independent graders. The average retinal thickness per macular region is compared with the automated Stratus OCT results. Intergrader and intragrader reproducibility is calculated by Bland-Altman plots of the mean difference between both gradings and by Pearson correlation coefficients. In addition, the correlation between Stratus OCT and our methodology-derived thickness is also presented. The mean thickness difference between Stratus OCT and our methodology is 6.53 microm and 26.71 microm when using the inner segment/outer segment (IS/OS) junction and outer segment/retinal pigment epithelium (OS/RPE) junction as the outer retinal border, respectively. Overall, the median of the thickness differences as a percentage of the mean thickness is less than 1% and 2% for the intragrader and intergrader reproducibility test, respectively. The measurement accuracy range of the OCT retinal image analysis (OCTRIMA) algorithm is between 0.27 and 1.47 microm and 0.6 and 1.76 microm for the intragrader and intergrader reproducibility tests, respectively. Pearson correlation coefficients demonstrate R(2)>0.98 for all Early Treatment Diabetic Retinopathy Study (ETDRS) regions. Our methodology facilitates a more robust and localized quantification of the retinal structure in normal healthy controls and patients with clinically significant intraretinal features.
authors.affiliation: University of Miami, Miller School of Medicine, Bascom Palmer Eye Institute, Miami, Florida 33136, USA. dcabrera2@med.miami.edu
authors.forename: Delia Cabrera
authors.initials: DC
authors.lastname: Debuc
authors.forename: Harry M
authors.initials: HM
authors.lastname: Salinas
authors.forename: Sudarshan
authors.initials: S
authors.lastname: Ranganathan
authors.forename: Erika
authors.initials: E
authors.lastname: Tátrai
authors.forename: Wei
authors.initials: W
authors.lastname: Gao
authors.forename: Meixiao
authors.initials: M
authors.lastname: Shen
authors.forename: Jianhua
authors.initials: J
authors.lastname: Wang
authors.forename: Gábor M
authors.initials: GM
authors.lastname: Somfai
authors.forename: Carmen A
authors.initials: CA
authors.lastname: Puliafito
mesh_terms: D000465:Algorithms; D001185:Artificial Intelligence; D006801:Humans; D007089:Image Enhancement / Q000379:methods*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D010363:Pattern Recognition, Automated / Q000379:methods*; D015203:Reproducibility of Results; D012160:Retina / Q000033:anatomy & histology*; D042262:Retinoscopy / Q000379:methods*; D012680:Sensitivity and Specificity; D041623:Tomography, Optical Coherence / Q000379:methods*
references.citation: Lancet. 1986 Feb 8;1(8476):307-10
references.pmid: 2868172
references.citation: Invest Ophthalmol Vis Sci. 2009 Jul;50(7):3432-7
references.pmid: 19234346
references.citation: Invest Ophthalmol Vis Sci. 2007 Feb;48(2):839-48
references.pmid: 17251486
references.citation: Opt Express. 2005 Dec 12;13(25):10200-16
references.pmid: 19503235
references.citation: J Biomed Opt. 2009 Nov-Dec;14(6):064023
references.pmid: 20059261
references.citation: Opt Express. 2005 Nov 14;13(23):9480-91
references.pmid: 19503151
references.citation: IEEE Trans Med Imaging. 2001 Sep;20(9):900-16
references.pmid: 11585207
references.citation: Med Eng Phys. 2007 May;29(4):432-41
references.pmid: 16860587
references.citation: Am J Ophthalmol. 2009 Feb;147(2):364-373.e2
references.pmid: 18848317
references.citation: Am J Ophthalmol. 2005 Jun;139(6):1056-61
references.pmid: 15953436
references.citation: Invest Ophthalmol Vis Sci. 2005 Jun;46(6):2012-7
references.pmid: 15914617
references.citation: Invest Ophthalmol Vis Sci. 2007 Sep;48(9):4300-7
references.pmid: 17724220
references.citation: J Biomed Opt. 2007 Jul-Aug;12(4):041207
references.pmid: 17867796
references.citation: IEEE Trans Med Imaging. 2007 Jun;26(6):761-71
references.pmid: 17679327
references.citation: Arch Ophthalmol. 2005 Jul;123(7):939-43
references.pmid: 16009835
references.citation: Opt Express. 2009 Aug 31;17(18):15659-69
references.pmid: 19724565
references.citation: IEEE Trans Med Imaging. 2005 Aug;24(8):929-45
references.pmid: 16092326
references.citation: Opt Express. 2008 Oct 13;16(21):16410-22
references.pmid: 18852747
references.citation: Arch Ophthalmol. 1995 May;113(5):655-60
references.pmid: 7748138
references.citation: Science. 1991 Nov 22;254(5035):1178-81
references.pmid: 1957169
references.citation: Ophthalmology. 1991 May;98(5 Suppl):741-56
references.pmid: 2062510
references.citation: IEEE Trans Vis Comput Graph. 2007 Nov-Dec;13(6):1719-26
references.pmid: 17968130
references.citation: Br J Ophthalmol. 2008 Feb;92(2):197-203
references.pmid: 17965102
references.citation: Invest Ophthalmol Vis Sci. 2010 Apr;51(4):1988-93
references.pmid: 19933178
references.citation: IEEE Trans Med Imaging. 2008 Oct;27(10):1495-505
references.pmid: 18815101
references.citation: Prog Retin Eye Res. 2006 May;25(3):325-53
references.pmid: 16716639