title: Species-specific control of cellular proliferation and the impact of large animal models for the use of olfactory ensheathing cells and Schwann cells in spinal cord repair.
abstract: Autologous transplantation of olfactory ensheathing cells (OECs) and Schwann cells (SCs) is considered a promising option to promote axonal regrowth and remyelination after spinal cord injury in humans. However, if the experimental data from the rodent model can be directly extrapolated to humans, as widely believed, remains to be established. While limitations of the rodent system have recently been discussed with regard to the distinct organization of the motor systems, the question whether OECs and SCs may display species-specific properties has not been fully addressed. Prompted by recent studies on canine and porcine glia, we performed a detailed analysis of the in vitro and in vivo properties of OECs and SCs and show that rodent but not human, monkey, porcine, and canine glia require mitogens for in vitro expansion, display a complex response to elevated intracellular cAMP, and undergo spontaneous immortalization upon prolonged mitogen stimulation. These data indicate fundamental inter-species differences of the control of cellular proliferation. Whether OECs and SCs from large animals and humans share growth-promoting in vivo properties with their rodent counterpart is not yet clear. Autologous implantation studies in humans did not reveal adverse effects of cell transplantation so far. However, in vivo studies of large animal or human glia and rodent recipients mainly focused on the remyelinating potential of the transplanted cells. Thus, further experimental in vivo studies in large animals are essential to fully define the axonal growth-promoting potential of OECs and SCs. Based on the homology of the in vitro growth control between porcine, canine and human glia, it is concluded that these species may serve as valuable translational models for scaling up human procedures. This article is part of a Special Issue entitled: Understanding olfactory ensheathing glia and their prospect for nervous system repair.
authors.affiliation: Department of Pathology, University of Veterinary Medicine, Hannover, Germany. konstantin.wewetzer@tiho-hannover.de
authors.forename: Konstantin
authors.initials: K
authors.lastname: Wewetzer
authors.forename: Christine
authors.initials: C
authors.lastname: Radtke
authors.forename: Jeffery
authors.initials: J
authors.lastname: Kocsis
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Baumgärtner
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D017690:Cell Transplantation / Q000379:methods; D004195:Disease Models, Animal; D004285:Dogs; D000882:Haplorhini; D006801:Humans; D009830:Olfactory Bulb / Q000166:cytology / Q000637:transplantation*; D012583:Schwann Cells / Q000166:cytology / Q000637:transplantation*; D013045:Species Specificity; D013119:Spinal Cord Injuries / Q000473:pathology / Q000601:surgery*; D058630:Spinal Cord Regeneration / Q000502:physiology; D013552:Swine