title: Rapid detection of lactate dehydrogenase and genotyping of Plasmodium falciparum in saliva of children with acute uncomplicated malaria.
abstract: The diagnosis of malaria in biological fluids other than blood using non-invasive, rapid diagnostic techniques provides a valuable approach in case management and epidemiological studies of malaria. Rapid detection of Plasmodium falciparum lactate dehydrogenase (pLDH) in saliva samples from 130 of 144 children with microscopically confirmed P. falciparum infection was evaluated using Optimal-IT dipsticks. Genotyping of parasites was also performed in saliva and blood samples from a cohort of patients by polymerase chain reaction (PCR). The sensitivity of the dipstick in whole-blood, whole-saliva, or supernatant of spun saliva samples was 97.2%, 77.9%, and 48.4%, respectively. The sensitivity of the dipstick in whole-saliva samples was significantly higher than in supernatant of spun saliva samples (P < 0.0005). Mutant T76 allele was detectable in 60% and 57% of blood and saliva samples, respectively. This finding shows rapid detection of pLDH in patient saliva.
authors.affiliation: Department of Pharmacology and Therapeutics, College of Medicine, University of Ibadan, Ibadan, Nigeria. solagbotosho@yahoo.co.uk
authors.forename: Grace O
authors.initials: GO
authors.lastname: Gbotosho
authors.forename: Christian T
authors.initials: CT
authors.lastname: Happi
authors.forename: Onikepe
authors.initials: O
authors.lastname: Folarin
authors.forename: Ochuko
authors.initials: O
authors.lastname: Keyamo
authors.forename: Akintunde
authors.initials: A
authors.lastname: Sowunmi
authors.forename: Ayoade M J
authors.initials: AM
authors.lastname: Oduola
mesh_terms: D000818:Animals; D002648:Child; D002675:Child, Preschool; D005838:Genotype; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000032:analysis*; D010963:Plasmodium falciparum / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D012463:Saliva / Q000469:parasitology*
chemical_list: D007770:L-Lactate Dehydrogenase
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