Reactive oxygen species (ROS) reduce the expression of BRAK/CXCL14 in human head and neck squamous cell carcinoma cells.
The present study investigated the effects of oxidative stress induced by reactive oxygen species (ROS), such as hydrogen peroxide (H(2)O(2)) and hydroxyl radical (HO(*)), on the expression of both BRAK , which is also known as non-ELR motif angiostatic CXC chemokine ligand 14 (CXCL14), in head and neck squamous cell carcinoma (HNSCC) cells. When HNSCC cells were cultured in the presence of ROS, the expression of BRAK was significantly decreased whereas that of IL-8 was increased. Interestingly, the effects on the expression of both genes in HNSCC cells were much greater with HO(blacksquare, square, filled) than with H(2)O(2). The effects of ROS on both BRAK and IL-8 expression were attenuated by pre-treatment with N-acetyl-L-cysteine (NAC), epidermal growth factor receptor (EGFR), and mitogen-activated protein kinase (MAPK) inhibitors. These results indicate that oxidative stress induced by H(2)O(2) or HO(*) stimulates angiogenesis and tumuor progression by altering the gene expression of BRAK and IL-8 via the EGFR/MEK/ERK pathway in human HNSCC cells.
Maehata
Yojiro
Y
Department of Clinical Care Medicine Division of Pharmacology and ESR Laboratories, Kanagawa Dental College, Yokosuka, 238-8580, Japan.
Ozawa
Shigeyuki
S
Kobayashi
Kyo
K
Kato
Yasumasa
Y
Yoshino
Fumihiko
F
Miyamoto
Chihiro
C
Izukuri
Kazuhito
K
Kubota
Eiro
E
Hata
Ryu-Ichiro
R
Lee
Masaichi-Chang-Il
MC
D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D002470:Cell Survival / Q000187:drug effects; D019743:Chemokines, CXC / Q000235:genetics* / Q000378:metabolism; D005296:Ferrous Compounds / Q000494:pharmacology; D006258:Head and Neck Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism / Q000494:pharmacology; D017665:Hydroxyl Radical / Q000032:analysis / Q000378:metabolism; D016209:Interleukin-8 / Q000096:biosynthesis; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D014407:Tumor Cells, Cultured
C118056:CXCL14 protein, human; D019743:Chemokines, CXC; D005296:Ferrous Compounds; D016209:Interleukin-8; D017382:Reactive Oxygen Species; D017665:Hydroxyl Radical; C020748:ferrous sulfate; D006861:Hydrogen Peroxide