title: Highly sensitive protein detection based on aptamer probe and isothermal nicking enzyme assisted fluorescence signal amplification.
abstract: An isothermal and sensitive fluorescence assay for protein detection using aptamer-protein-aptamer conjugates based on nicking enzyme amplification has been developed, which was combined with magnetic microparticles separation, making this assay suitable for protein detection in biological samples.
authors.affiliation: MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, 510631 Guangzhou, China.
authors.forename: Liyun
authors.initials: L
authors.lastname: Xue
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Zhou
authors.forename: Da
authors.initials: D
authors.lastname: Xing
mesh_terms: D052157:Aptamers, Nucleotide; D001483:Base Sequence; D005456:Fluorescent Dyes; D057230:Limit of Detection; D011506:Proteins / Q000032:analysis*; D013050:Spectrometry, Fluorescence
chemical_list: D052157:Aptamers, Nucleotide; D005456:Fluorescent Dyes; D011506:Proteins