Resolving the assembly state of herpes simplex virus during axon transport by live-cell imaging.
Neurotropic herpesviruses depend on long-distance axon transport for the initial establishment of latency in peripheral ganglia (retrograde transport) and for viral spread in axons to exposed body surfaces following reactivation (anterograde transport). Images of neurons infected with herpes simplex virus type 1 (HSV-1), acquired using electron microscopy, have led to a debate regarding why different types of viral structures are seen in axons and which of these particles are relevant to the axon transport process. In this study, we applied time-lapse fluorescence microscopy to image HSV-1 virion components actively translocating to distal axons in primary neurons and neuronal cell lines. Key to these findings, only a small fraction of viral particles were engaged in anterograde transport during the egress phase of infection at any given time. By selective analysis of the composition of the subpopulation of actively transporting capsids, a link between transport of fully assembled HSV-1 virions and the neuronal secretory pathway was identified. Last, we have evaluated the seemingly opposing findings made in previous studies of HSV-1 axon transport in fixed cells and demonstrate a limitation to assessing the composition of individual HSV-1 particles using antibody detection methods.
Antinone
Sarah E
SE
Department of Microbiology-Immunology, Northwestern University Feinberg School of Medicine, 303 E. Chicago Ave., Chicago, IL 60611, USA.
Zaichick
Sofia V
SV
Smith
Gregory A
GA
D000818:Animals; D001370:Axonal Transport; D001369:Axons / Q000821:virology*; D015153:Blotting, Western; D002213:Capsid / Q000378:metabolism; D002478:Cells, Cultured; D002642:Chick Embryo; D002522:Chlorocebus aethiops; D005455:Fluorescent Antibody Technique; D006561:Herpes Simplex / Q000821:virology*; D008854:Microscopy, Electron; D009474:Neurons / Q000821:virology*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018139:Simplexvirus / Q000502:physiology*; D014709:Vero Cells; D014759:Viral Envelope Proteins / Q000378:metabolism; D014771:Virion / Q000502:physiology*; D014779:Virus Replication
D012333:RNA, Messenger; D014759:Viral Envelope Proteins
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