title: Laser capture microdissection for gene expression analysis of inner cell mass and trophectoderm from blastocysts.
abstract: Isolation of pure inner cell mass (ICM) and trophectoderm (TE) samples from a single blastocyst is necessary to obtain accurate information on the transcriptomes of these cells. Laser capture microdissection (LCM) provides the possibility to isolate small tissue fractions from heterogeneous tissue sections without contamination by the surrounding tissue and without changing the gene expression pattern of the cells. However, the small size of blastocysts hampers tissue processing prior to LCM. This article describes a protocol for the application of LCM to isolate homogeneous ICM and TE cell samples from single bovine blastocysts for downstream gene expression analysis.
authors.affiliation: Department of Reproduction, Obstetrics, and Herd Health, Ghent University, Merelbeke, Belgium. muriel.filliers@ugent.be
authors.forename: Muriel
authors.initials: M
authors.lastname: Filliers
authors.forename: Ward
authors.initials: W
authors.lastname: De Spiegelaere
authors.forename: Luc
authors.initials: L
authors.lastname: Peelman
authors.forename: Karen
authors.initials: K
authors.lastname: Goossens
authors.forename: Christian
authors.initials: C
authors.lastname: Burvenich
authors.forename: Leen
authors.initials: L
authors.lastname: Vandaele
authors.forename: Pieter
authors.initials: P
authors.lastname: Cornillie
authors.forename: Ann
authors.initials: A
authors.lastname: Van Soom
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000378:metabolism*; D053624:Blastocyst Inner Cell Mass / Q000378:metabolism*; D002417:Cattle; D004587:Electrophoresis, Agar Gel / Q000379:methods; D020869:Gene Expression Profiling; D007834:Lasers; D042282:Microdissection / Q000379:methods*; D012337:RNA, Ribosomal, 18S / Q000235:genetics / Q000378:metabolism
chemical_list: D012337:RNA, Ribosomal, 18S