A recombinant AeDNA containing the insect-specific toxin, BmK IT1, displayed an increasing pathogenicity on Aedes albopictus.
The Aedes aegypti densovirus (AeDNV) has previously shown potential in mosquito control. To improve its efficacy as a biopesticide, the gene for an excitatory insect-specific toxin from Buthus martensii Karsch (BmK IT1) was inserted into the AeDNV genome and cloned into pUCA plasmid. The coding sequence for green fluorescent protein was ligated to the C-terminus of the BmK IT1 gene as a screening marker. Recombinant and helper plasmids were cotransfected into C6/36 cells; wild-type viruses were the controls. The recombinant viruses were identified and quantified by real-time polymerase chain reaction and exposed to Ae. albopictus larvae for the evaluation of its bioinsecticidal activity. LT(50) and LD(50) bioassays showed that the recombinant AeDNV had stronger and faster pathogenic effects on Ae. albopictus than the wild-type virus. This is the first report on the recombinant AeDNA containing the insect-specific toxin, BmK IT1, which may be used to develop a novel type of insecticide.
Gu
Jin-Bao
JB
School of Public Health and Tropical Medicine, Southern Medical University, Guangdong, People's Republic of China. gujinbao@fimmu.com
Dong
Yun-Qiao
YQ
Peng
Hong-Juan
HJ
Chen
Xiao-Guang
XG
D000330:Aedes / Q000187:drug effects* / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D017931:DNA Primers; D004274:DNA, Recombinant / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D016133:Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012604:Scorpion Venoms / Q000235:genetics / Q000494:pharmacology*
C471081:BmK Ita1 toxin, Buthus martensii; D017931:DNA Primers; D004274:DNA, Recombinant; D012604:Scorpion Venoms
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