title: Expression and functional analysis of two genes encoding transcription factors, VpWRKY1 and VpWRKY2, isolated from Chinese wild Vitis pseudoreticulata.
abstract: In this study, two WRKY genes were isolated from Erysiphe necator-resistant Chinese wild Vitis pseudoreticulata W. T. Wang 'Baihe-35-1', and designated as VpWRKY1 (GenBank accession no. GQ884198) and VpWRKY2 (GenBank accession no. GU565706). Nuclear localization of the two proteins was demonstrated in onion epidermal cells, while trans-activation function was confirmed in the leaves of 'Baihe-35-1'. Expression of VpWRKY1 and VpWRKY2 was induced rapidly by salicylic acid treatment in 'Baihe-35-1'. Expression of VpWRKY1 and VpWRKY2 was also induced rapidly by E. necator infection in 11 grapevine genotypes; the maximum induction of VpWRKY1 was greater in E. necator-resistant grapevine genotypes than in susceptible ones post E. necator inoculation. Furthermore, ectopic expression of VpWRKY1 or VpWRKY2 in Arabidopsis enhanced resistance to powdery mildew Erysiphe cichoracearum, and enhanced salt tolerance of transgenic plants. VpWRKY2 also enhanced cold tolerance of transgenic plants. In addition, the two proteins were shown to regulate the expression of some defense marker genes in Arabidopsis and grapevine. The data suggest that VpWRKY1 and VpWRKY2 may underlie the resistance in transgenic grapevine to E. necator and tolerance to salt and cold stresses.
authors.affiliation: College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, People's Republic of China.
authors.forename: Huie
authors.initials: H
authors.lastname: Li
authors.forename: Yan
authors.initials: Y
authors.lastname: Xu
authors.forename: Yu
authors.initials: Y
authors.lastname: Xiao
authors.forename: Ziguo
authors.initials: Z
authors.lastname: Zhu
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Xie
authors.forename: Heqing
authors.initials: H
authors.lastname: Zhao
authors.forename: Yuejin
authors.initials: Y
authors.lastname: Wang
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D030821:Plants, Genetically Modified; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013347:Subcellular Fractions / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D027843:Vitis / Q000235:genetics* / Q000378:metabolism
chemical_list: D017931:DNA Primers; D014157:Transcription Factors
references.citation: BMC Plant Biol. 2008 Dec 22;8:132
references.pmid: 19102779
references.citation: Plant J. 2005 Apr;42(1):95-110
references.pmid: 15773856
references.citation: Cell Res. 2008 Apr;18(4):508-21
references.pmid: 18071364
references.citation: Planta. 2007 Jun;226(1):125-37
references.pmid: 17310369
references.citation: Mol Plant Microbe Interact. 2007 Feb;20(2):120-8
references.pmid: 17313163
references.citation: BMC Plant Biol. 2008 Jun 20;8:68
references.pmid: 18570649
references.citation: Planta. 2009 Nov;230(6):1155-66
references.pmid: 19760263
references.citation: Nucleic Acids Res. 2008 Jan;36(Database issue):D966-9
references.pmid: 17933783
references.citation: Plant Mol Biol. 2004 May;55(3):399-416
references.pmid: 15604689
references.citation: Theor Appl Genet. 2009 Dec;120(1):163-76
references.pmid: 19821064
references.citation: Plant Physiol. 2002 Nov;130(3):1143-51
references.pmid: 12427981
references.citation: Nature. 2007 Sep 27;449(7161):463-7
references.pmid: 17721507
references.citation: Plant J. 1997 Oct;12(4):757-68
references.pmid: 9375391
references.citation: Plant J. 2001 Sep;27(6):581-90
references.pmid: 11576441
references.citation: J Plant Physiol. 2007 Aug;164(8):969-79
references.pmid: 16919842
references.citation: Trends Plant Sci. 2005 Feb;10(2):71-8
references.pmid: 15708344
references.citation: Plant Cell. 2006 Nov;18(11):3289-302
references.pmid: 17114354
references.citation: Plant Mol Biol. 2010 Jan;72(1-2):215-34
references.pmid: 19902151
references.citation: Plant J. 1998 Dec;16(6):735-43
references.pmid: 10069079
references.citation: Curr Opin Plant Biol. 2004 Oct;7(5):491-8
references.pmid: 15337090
references.citation: PLoS One. 2007 Dec 19;2(12):e1326
references.pmid: 18094749
references.citation: Physiol Plant. 2007 Nov;131(3):434-47
references.pmid: 18251882
references.citation: Plant Mol Biol. 2003 Jan;51(1):21-37
references.pmid: 12602888
references.citation: Curr Opin Plant Biol. 2007 Aug;10(4):366-71
references.pmid: 17644023
references.citation: J Exp Bot. 2007;58(8):1999-2010
references.pmid: 17456504
references.citation: Annu Rev Phytopathol. 2005;43:205-27
references.pmid: 16078883
references.citation: Nature. 2002 Aug 8;418(6898):696-9
references.pmid: 12167877
references.citation: Curr Opin Plant Biol. 2002 Oct;5(5):430-6
references.pmid: 12183182
references.citation: J Agric Food Chem. 2008 Jul 23;56(14):5781-7
references.pmid: 18570435
references.citation: Curr Opin Plant Biol. 2004 Aug;7(4):465-71
references.pmid: 15231271
references.citation: Trends Plant Sci. 2000 May;5(5):199-206
references.pmid: 10785665
references.citation: Plant Physiol. 2009 Aug;150(4):1648-55
references.pmid: 19420325
references.citation: Plant Cell. 2004 Feb;16(2):319-31
references.pmid: 14742872
references.citation: Plant Cell. 2006 May;18(5):1310-26
references.pmid: 16603654
references.citation: Biochem Biophys Res Commun. 2007 Jul 27;359(2):234-8
references.pmid: 17533111
references.citation: Protoplasma. 2011 Apr;248(2):415-23
references.pmid: 20512385
references.citation: BMC Plant Biol. 2009 Jul 22;9:96
references.pmid: 19622176
references.citation: Plant Biotechnol J. 2008 Jun;6(5):486-503
references.pmid: 18384508
references.citation: BMC Genet. 2009 Dec 30;10:89
references.pmid: 20042081
references.citation: Plant Physiol. 2008 Jan;146(1):236-49
references.pmid: 17993546
references.citation: Planta. 2010 Jan;231(2):475-87
references.pmid: 19937257
references.citation: Biochim Biophys Acta. 2007 Jul-Aug;1769(7-8):497-505
references.pmid: 17532485
references.citation: Mol Plant Microbe Interact. 2007 May;20(5):492-9
references.pmid: 17506327