title: Amyloid formation and disaggregation of α-synuclein and its tandem repeat (α-TR).
abstract: The aggregation of α-synuclein is clearly related to the pathogenesis of Parkinson's disease. Therefore, detailed understanding of the mechanism of fibril formation is highly valuable for the development of clinical treatment and also of the diagnostic tools. Here, we have investigated the interaction of α-synuclein with ionic liquids by using several biochemical techniques including Thioflavin T assays and transmission electron microscopy (TEM). Our data shows a rapid formation of α-synuclein amyloid fibrils was stimulated by 1-butyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide [BIMbF(3)Im], and these fibrils could be disaggregated by polyphenols such as epigallocatechin gallate (EGCG) and baicalein. Furthermore, the effect of [BIMbF(3)Im] on the α-synuclein tandem repeat (α-TR) in the aggregation process was studied.
authors.affiliation: Department of Molecular Science and Technology, Graduate School of Interdisciplinary Programs, Ajou University, Suwon 443-749, South Korea.
authors.forename: Song Yi
authors.initials: SY
authors.lastname: Bae
authors.forename: Seulgi
authors.initials: S
authors.lastname: Kim
authors.forename: Heejin
authors.initials: H
authors.lastname: Hwang
authors.forename: Hyun-Kyung
authors.initials: HK
authors.lastname: Kim
authors.forename: Hyun C
authors.initials: HC
authors.lastname: Yoon
authors.forename: Jae Ho
authors.initials: JH
authors.lastname: Kim
authors.forename: Sangyoon
authors.initials: S
authors.lastname: Lee
authors.forename: T Doohun
authors.initials: TD
authors.lastname: Kim
mesh_terms: D000682:Amyloid / Q000737:chemistry / Q000378:metabolism*; D052160:Benzothiazoles; D002392:Catechin / Q000031:analogs & derivatives / Q000494:pharmacology; D044950:Flavanones / Q000494:pharmacology; D006801:Humans; D007093:Imidazoles / Q000494:pharmacology*; D007094:Imides / Q000494:pharmacology*; D052578:Ionic Liquids / Q000737:chemistry; D046529:Microscopy, Electron, Transmission; D010300:Parkinson Disease / Q000378:metabolism*; D013449:Sulfonamides; D020080:Tandem Repeat Sequences; D013844:Thiazoles / Q000737:chemistry; D051844:alpha-Synuclein / Q000737:chemistry / Q000378:metabolism*
chemical_list: C493485:1-butyl-3-methylimidazolium bis((trifluoromethyl)sulfonyl)amide; D000682:Amyloid; D052160:Benzothiazoles; D044950:Flavanones; D007093:Imidazoles; D007094:Imides; D052578:Ionic Liquids; D013449:Sulfonamides; D013844:Thiazoles; D051844:alpha-Synuclein; C009462:thioflavin T; C006680:baicalein; D002392:Catechin; C045651:epigallocatechin gallate