title: Morphologies of mouse retinal ganglion cells expressing transcription factors Brn3a, Brn3b, and Brn3c: analysis of wild type and mutant cells using genetically-directed sparse labeling.
abstract: The mammalian retina contains more than 50 distinct neuronal types, which are broadly classified into several major classes: photoreceptor, bipolar, horizontal, amacrine, and ganglion cells. Although some of the developmental mechanisms involved in the differentiation of retinal ganglion cells (RGCs) are beginning to be understood, there is little information regarding the genetic and molecular determinants of the distinct morphologies of the 15-20 mammalian RGC cell types. Previous work has shown that the transcription factor Brn3b/Pou4f2 plays a major role in the development and survival of many RGCs. The roles of the closely related family members, Brn3a/Pou4f1 and Brn3c/Pou4f3 in RGC development are less clear. Using a genetically-directed method for sparse cell labeling and sparse conditional gene ablation in mice, we describe here the sets of RGC types in which each of the three Brn3/Pou4f transcription factors are expressed and the consequences of ablating these factors on the development of RGC morphologies.
authors.affiliation: Department of Molecular Biology and Genetics, Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA. tbadea@mail.jhmi.edu
authors.forename: Tudor Constantin
authors.initials: TC
authors.lastname: Badea
authors.forename: Jeremy
authors.initials: J
authors.lastname: Nathans
mesh_terms: D000818:Animals; D005838:Genotype; D051379:Mice; D012165:Retinal Ganglion Cells / Q000166:cytology* / Q000502:physiology*; D050819:Transcription Factor Brn-3A / Q000235:genetics / Q000378:metabolism*; D050820:Transcription Factor Brn-3B / Q000235:genetics / Q000378:metabolism*; D050821:Transcription Factor Brn-3C / Q000235:genetics / Q000378:metabolism*
chemical_list: D050819:Transcription Factor Brn-3A; D050820:Transcription Factor Brn-3B; D050821:Transcription Factor Brn-3C
references.citation: Nat Rev Neurosci. 2004 Oct;5(10):747-57
references.pmid: 15378035
references.citation: Neuron. 2010 Jul 15;67(1):49-60
references.pmid: 20624591
references.citation: Nature. 2008 May 1;453(7191):102-5
references.pmid: 18432195
references.citation: Cell. 2002 Sep 6;110(5):649-60
references.pmid: 12230981
references.citation: J Comp Neurol. 2005 Sep 12;490(1):29-39
references.pmid: 16041717
references.citation: Nat Neurosci. 2009 Sep;12(9):1197-204
references.pmid: 19648912
references.citation: J Neurosci. 2003 Jul 9;23(14):6063-73
references.pmid: 12853425
references.citation: Eur J Neurosci. 2007 Jul;26(2):367-80
references.pmid: 17650112
references.citation: Prog Retin Eye Res. 2002 May;21(3):263-302
references.pmid: 12052385
references.citation: PLoS One. 2008 Feb 06;3(2):e1533
references.pmid: 18253481
references.citation: J Physiol. 1974 Jul;240(2):421-56
references.pmid: 4421622
references.citation: J Comp Neurol. 2004 Dec 20;480(4):331-51
references.pmid: 15558785
references.citation: Nature. 2008 Mar 27;452(7186):478-82
references.pmid: 18368118
references.citation: J Comp Neurol. 2005 May 9;485(3):191-201
references.pmid: 15791644
references.citation: Proc Natl Acad Sci U S A. 1996 Apr 30;93(9):3920-5
references.pmid: 8632990
references.citation: Science. 1976 Oct 8;194(4261):193-5
references.pmid: 959847
references.citation: Annu Rev Neurosci. 1984;7:13-41
references.pmid: 6370078
references.citation: J Physiol. 1974 Jul;240(2):457-92
references.pmid: 4420300
references.citation: Science. 2002 Feb 8;295(5557):1065-70
references.pmid: 11834834
references.citation: Neuron. 2000 Dec;28(3):779-92
references.pmid: 11163266
references.citation: J Comp Neurol. 1990 Dec 15;302(3):675-93
references.pmid: 1702124
references.citation: Nature. 1994 Feb 24;367(6465):731-5
references.pmid: 8107868
references.citation: PLoS One. 2009 Nov 16;4(11):e7859
references.pmid: 19924248
references.citation: J Neurosci. 2009 Jul 1;29(26):8372-87
references.pmid: 19571128
references.citation: Brain Res. 1984 Apr 23;298(1):187-90
references.pmid: 6722555
references.citation: Nature. 2008 Jan 24;451(7177):470-4
references.pmid: 18216855
references.citation: Curr Biol. 2007 Aug 7;17(15):R577-82
references.pmid: 17686423
references.citation: Cell. 2010 Apr 30;141(3):536-48
references.pmid: 20434990
references.citation: Mol Cell Neurosci. 2000 Aug;16(2):141-56
references.pmid: 10924257
references.citation: Nat Neurosci. 2006 Jan;9(1):85-92
references.pmid: 16341211
references.citation: Neuron. 2003 Jan 9;37(1):15-27
references.pmid: 12526769
references.citation: Proc Natl Acad Sci U S A. 1997 Aug 19;94(17):9445-50
references.pmid: 9256502
references.citation: Vis Neurosci. 2009 Mar-Apr;26(2):195-213
references.pmid: 19272195
references.citation: J Physiol. 1964 Oct;173:377-407
references.pmid: 14220259
references.citation: Neuron. 2000 Sep;27(3):513-23
references.pmid: 11055434
references.citation: Science. 2002 Feb 8;295(5557):1070-3
references.pmid: 11834835
references.citation: J Neurosci. 2008 Mar 26;28(13):3392-403
references.pmid: 18367606
references.citation: J Comp Neurol. 1990 Dec 15;302(3):657-74
references.pmid: 1702123
references.citation: J Physiol. 1974 Jul;240(2):397-419
references.pmid: 4422168
references.citation: J Comp Neurol. 2000 Aug 14;424(1):1-23
references.pmid: 10888735
references.citation: J Comp Neurol. 2009 Feb 10;512(5):664-87
references.pmid: 19051243
references.citation: Nature. 2008 Jan 24;451(7177):465-9
references.pmid: 18216854
references.citation: J Neurophysiol. 2003 Sep;90(3):1704-13
references.pmid: 12966177
references.citation: J Comp Neurol. 2005 Aug 29;489(3):293-310
references.pmid: 16025455
references.citation: Neuroscience. 2006 Jun 19;140(1):123-36
references.pmid: 16626866
references.citation: Nat Neurosci. 2009 Oct;12(10):1308-16
references.pmid: 19734895
references.citation: Nature. 1996 Jun 13;381(6583):603-6
references.pmid: 8637595
references.citation: J Comp Neurol. 2002 Sep 16;451(2):115-26
references.pmid: 12209831
references.citation: J Neurosci. 2002 May 1;22(9):3831-43
references.pmid: 11978858
references.citation: Neuron. 2009 Mar 26;61(6):852-64
references.pmid: 19323995
references.citation: J Comp Neurol. 2004 Jan 26;469(1):70-82
references.pmid: 14689473
references.citation: Curr Opin Neurobiol. 2001 Aug;11(4):431-6
references.pmid: 11502388
references.citation: J Neurosci. 2009 Mar 4;29(9):2706-24
references.pmid: 19261865
references.citation: Nature. 2009 Jan 15;457(7227):281-7
references.pmid: 19118382
references.citation: Nature. 1975 Apr 24;254(5502):659-62
references.pmid: 47614
references.citation: Development. 2002 Jan;129(2):467-77
references.pmid: 11807038
references.citation: J Physiol. 2006 Oct 1;576(Pt 1):197-202
references.pmid: 16901944
references.citation: Neuron. 2009 Nov 25;64(4):484-97
references.pmid: 19945391
references.citation: J Neurosci. 1996 May 1;16(9):2934-44
references.pmid: 8622124
references.citation: Neuron. 2008 Aug 14;59(3):425-38
references.pmid: 18701068
references.citation: J Neurosci. 2003 Mar 15;23(6):2314-22
references.pmid: 12657690
references.citation: J Physiol. 1967 Feb;188(3):285-307
references.pmid: 6032202
references.citation: J Neurosci. 1995 Jul;15(7 Pt 1):4762-85
references.pmid: 7623109
references.citation: J Physiol. 2005 Feb 1;562(Pt 3):915-23
references.pmid: 15564281
references.citation: PLoS One. 2009;4(1):e4320
references.pmid: 19177171
references.citation: J Comp Neurol. 1993 Dec 8;338(2):289-303
references.pmid: 8308173
references.citation: Nature. 2001 Mar 29;410(6828):583-7
references.pmid: 11279496
references.citation: J Comp Neurol. 2010 Jul 1;518(13):2405-22
references.pmid: 20503419
references.citation: Proc Natl Acad Sci U S A. 1996 Oct 15;93(21):11950-5
references.pmid: 8876243
references.citation: J Neurosci. 2010 Jan 27;30(4):1452-62
references.pmid: 20107072
references.citation: Neuron. 2009 May 14;62(3):327-34
references.pmid: 19447089