title: Rapid dopaminergic modulation of the fish hypothalamic transcriptome and proteome.
abstract: BACKGROUND
  Dopamine (DA) is a major neurotransmitter playing an important role in the regulation of vertebrate reproduction. We developed a novel method for the comparison of transcriptomic and proteomic data obtained from in vivo experiments designed to study the neuroendocrine actions of DA.
  
  
  RESULTS
  Female goldfish were injected (i.p.) with DA agonists (D1-specific; SKF 38393, or D2-specific; LY 171555) and sacrificed after 5 h. Serum LH levels were reduced by 57% and 75% by SKF 38393 and LY 171555, respectively, indicating that the treatments produced physiologically relevant responses in vivo. Bioinformatic strategies and a ray-finned fish database were established for microarray and iTRAQ proteomic analysis of the hypothalamus, revealing a total of 3088 mRNAs and 42 proteins as being differentially regulated by the treatments. Twenty one proteins and mRNAs corresponding to these proteins appeared on both lists. Many of the mRNAs and proteins affected by the treatments were grouped into the Gene Ontology categorizations of protein complex, signal transduction, response to stimulus, and regulation of cellular processes. There was a 57% and 14% directional agreement between the differentially-regulated mRNAs and proteins for SKF 38393 and LY 171555, respectively.
  
  
  CONCLUSIONS
  The results demonstrate the applicability of advanced high-throughput genomic and proteomic analyses in an amendable well-studied teleost model species whose genome has yet to be sequenced. We demonstrate that DA rapidly regulates multiple hypothalamic pathways and processes that are also known to be involved in pathologies of the central nervous system.
authors.affiliation: Department of Biology, Centre for Advanced Research in Environmental Genomics, University of Ottawa, Ottawa, Ontario, Canada.
authors.forename: Jason T
authors.initials: JT
authors.lastname: Popesku
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Martyniuk
authors.forename: Nancy D
authors.initials: ND
authors.lastname: Denslow
authors.forename: Vance L
authors.initials: VL
authors.lastname: Trudeau
mesh_terms: D000818:Animals; D004298:Dopamine / Q000378:metabolism*; D018491:Dopamine Agonists / Q000494:pharmacology; D004704:Endocrinology; D005260:Female; D020869:Gene Expression Profiling; D006054:Goldfish / Q000235:genetics* / Q000378:metabolism* / Q000502:physiology; D006801:Humans; D007031:Hypothalamus / Q000187:drug effects / Q000378:metabolism*; D007986:Luteinizing Hormone / Q000494:pharmacology; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012098:Reproduction; D013997:Time Factors
chemical_list: D018491:Dopamine Agonists; D020543:Proteome; D012333:RNA, Messenger; D007986:Luteinizing Hormone; D004298:Dopamine
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