The exon junction complex differentially marks spliced junctions.
The exon junction complex (EJC), which is deposited onto mRNAs as a consequence of splicing, is involved in multiple post-transcriptional events in metazoa. Here, using Drosophila melanogaster cells, we show that only some introns trigger EJC-dependent nonsense-mediated mRNA decay and that EJC association with particular spliced junctions depends on RNA cis-acting sequences. This study provides the first evidence to our knowledge that EJC deposition is not constitutive but instead is a regulated process.
Saulière
Jérôme
J
Institut de Biologie de l'Ecole Normale Supérieure, Centre National de la Recherche Scientifique/ UMR 8197, Paris, France.
Haque
Nazmul
N
Harms
Scot
S
Barbosa
Isabelle
I
Blanchette
Marco
M
Le Hir
Hervé
H
D020413:3' Untranslated Regions; D000818:Animals; D002460:Cell Line; D018388:Codon, Terminator; D029721:Drosophila Proteins / Q000378:metabolism; D004331:Drosophila melanogaster / Q000166:cytology / Q000235:genetics*; D039601:Eukaryotic Initiation Factor-4A / Q000378:metabolism; D005091:Exons / Q000235:genetics*; D017930:Genes, Reporter; D007438:Introns / Q000235:genetics; D049409:Luciferases, Firefly / Q000235:genetics; D049410:Luciferases, Renilla / Q000235:genetics; D008957:Models, Genetic; D046912:Multiprotein Complexes; D009687:Nuclear Proteins / Q000378:metabolism; D022821:RNA Splice Sites; D012326:RNA Splicing; D020871:RNA Stability; D012333:RNA, Messenger / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000378:metabolism
D020413:3' Untranslated Regions; D018388:Codon, Terminator; D029721:Drosophila Proteins; C107372:Mago protein, Drosophila; D046912:Multiprotein Complexes; D009687:Nuclear Proteins; D022821:RNA Splice Sites; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; C440016:tsu protein, Drosophila; D049410:Luciferases, Renilla; D049409:Luciferases, Firefly; D039601:Eukaryotic Initiation Factor-4A
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