title: Ultrasound-controlled cell aggregation in a multi-well chip.
abstract: We demonstrate a microplate platform for parallelized manipulation of particles or cells by frequency-modulated ultrasound. The device, consisting of a silicon-glass microchip and a single ultrasonic transducer, enables aggregation, positioning and high-resolution microscopy of cells distributed in an array of 100 microwells centered on the microchip. We characterize the system in terms of temperature control, aggregation and positioning efficiency, and cell viability. We use time-lapse imaging to show that cells continuously exposed to ultrasound are able to divide and remain viable for at least 12 hours inside the device. Thus, the device can be used to induce and maintain aggregation in a parallelized fashion, facilitating long-term microscopy studies of, e.g., cell-cell interactions.
authors.affiliation: Department of Applied Physics, Royal Institute of Technology, AlbaNova University Center, SE-106 91, Stockholm, Sweden.
authors.forename: Bruno
authors.initials: B
authors.lastname: Vanherberghen
authors.forename: Otto
authors.initials: O
authors.lastname: Manneberg
authors.forename: Athanasia
authors.initials: A
authors.lastname: Christakou
authors.forename: Thomas
authors.initials: T
authors.lastname: Frisk
authors.forename: Mathias
authors.initials: M
authors.lastname: Ohlin
authors.forename: Hans M
authors.initials: HM
authors.lastname: Hertz
authors.forename: Björn
authors.initials: B
authors.lastname: Önfelt
authors.forename: Martin
authors.initials: M
authors.lastname: Wiklund
mesh_terms: D000818:Animals; D002449:Cell Aggregation / Q000502:physiology*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005434:Flow Cytometry / Q000295:instrumentation*; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D008846:Micromanipulation / Q000295:instrumentation*; D014465:Ultrasonics / Q000295:instrumentation*