In vitro cellular responses in the RTG-2 cell line to complex mixtures of dioxins and dioxin-like PCDDs, PCDFs and PCBs.
High-resolution gas chromatography/mass spectrometry (HRGC/MS) is the standard method for analysing dioxin, furan and polybrominated retardants in hazardous waste. Determination of dioxin-like compounds using in vitro bioassays such as ethoxyresorufin-O-deethylase (EROD) is an important tool to evaluate their Ah receptor-mediated toxic effects, because it detects all arylhydrocarbon receptor ligands in a variety of sample matrices. In the present work, we compared RTG-2 cell line EROD bioassay with HRGC/MS for assessing waste samples (liquid and solid) contaminated with polychlorinated dibenzo-p-dioxins and dibenzofurans, polychlorinated biphenyls (dioxin-like PCBs) and other xenobiotics. For liquid samples, HRGC/MS-toxic equivalent (HRGC/MS-TEQ) values ranged from 273.26 to 5.84 ng TEQ l(-1) and correlated well (correlation coefficient 0.99) with values obtained by EROD-TEQ, which ranged from 128 to 2.5 ng TEQ l(-1). For solid samples, HRGC/MS-TEQ values ranged from 3.44 to 0.49 ng TEQ g(-1) and correlated less well than liquid samples (correlation coefficient 0.64) with values obtained by EROD-TEQ ranging from 2.27 to 0.93 ng TEQ g(-1). The overestimation of RTG-2 EROD-TEQ (1.2 +/- 0.92 of values established by HRGC/MS) and the absence of false-negative results may limit analytical costs by eliminating the need for follow-up GC/MS analysis on the negative samples. We suggest that RTG-2 EROD bioassay is an inexpensive means for preliminary dioxin and furan positive screenings of waste samples.
Babín
María del Mar
Mdel M
INIA, Department of Environment, Crta de La Coruña Km 7, 28040 Madrid, Spain. babin@inia.es
Sanz
Paloma
P
Concejero
Miguel Angel
MA
Martínez
María Angeles
MA
Tarazona
José Vicente
JV
D000818:Animals; D001572:Benzofurans / Q000032:analysis*; D001681:Biological Assay / Q000379:methods; D018929:Cell Culture Techniques; D002460:Cell Line; D045424:Complex Mixtures / Q000032:analysis; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism*; D004147:Dioxins / Q000032:analysis*; D004305:Dose-Response Relationship, Drug; D004790:Enzyme Induction; D008401:Gas Chromatography-Mass Spectrometry; D006256:Hazardous Waste / Q000032:analysis*; D017686:Oncorhynchus mykiss; D011078:Polychlorinated Biphenyls / Q000032:analysis*; D000072317:Polychlorinated Dibenzodioxins / Q000031:analogs & derivatives / Q000032:analysis; D011108:Polymers / Q000032:analysis*
D001572:Benzofurans; D045424:Complex Mixtures; D004147:Dioxins; D006256:Hazardous Waste; D000072317:Polychlorinated Dibenzodioxins; D011108:Polymers; C016068:polychlorodibenzofuran; D011078:Polychlorinated Biphenyls; D019363:Cytochrome P-450 CYP1A1