FBI-1 functions as a novel AR co-repressor in prostate cancer cells.
The pro-oncogene FBI-1, encoded by Zbtb7a, is a transcriptional repressor that belongs to the POK (POZ/BTB and Krüppel) protein family. In this study, we investigated a potential interaction between androgen receptor (AR) signaling and FBI-1 and demonstrated that overexpression of FBI-1 inhibited ligand-dependent AR activation. A protein-protein interaction was identified between FBI-1 and AR in a ligand-dependent manner. Furthermore, FBI-1, AR and SMRT formed a ternary complex and FBI-1 enhanced the recruitment of NCoR and SMRT to endogenous PSA upstream sequences. Our data also indicated that the FBI-1-mediated inhibition of AR transcriptional activity is partially dependent on HDAC. Interestingly, FBI-1 plays distinct roles in regulating LNCaP (androgen-dependent) and PC-3 cell (androgen-independent) proliferation.
Cui
Jiajun
J
Institute of Disease Control and Prevention, Chinese Academy of Military Medical Sciences, Beijing, People's Republic of China.
Yang
Yutao
Y
Zhang
Chuanfu
C
Hu
Pinliang
P
Kan
Wei
W
Bai
Xianhong
X
Liu
Xuelin
X
Song
Hongbin
H
D045744:Cell Line, Tumor; D047369:Chromatin Immunoprecipitation; D017931:DNA Primers / Q000235:genetics; D004268:DNA-Binding Proteins / Q000378:metabolism*; D006655:Histone Deacetylases / Q000378:metabolism; D006801:Humans; D047468:Immunoprecipitation; D008156:Luciferases; D008297:Male; D046912:Multiprotein Complexes / Q000378:metabolism*; D056985:Nuclear Receptor Co-Repressor 2 / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics; D011471:Prostatic Neoplasms / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000235:genetics; D011944:Receptors, Androgen / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000502:physiology*; D014157:Transcription Factors / Q000378:metabolism*; D014162:Transfection
D017931:DNA Primers; D004268:DNA-Binding Proteins; D046912:Multiprotein Complexes; C539130:NCOR2 protein, human; D056985:Nuclear Receptor Co-Repressor 2; D034741:RNA, Small Interfering; D011944:Receptors, Androgen; D014157:Transcription Factors; C485514:ZBTB7A protein, human; D008156:Luciferases; D006655:Histone Deacetylases
J Clin Oncol. 2002 Jul 1;20(13):3001-15
12089231
Biochem Soc Trans. 2006 Dec;34(Pt 6):1054-7
17073749
Clin Cancer Res. 2006 Mar 15;12(6):1665-71
16551847
Proc Natl Acad Sci U S A. 2003 Mar 4;100(5):2226-30
12589022
J Biol Chem. 2005 Feb 25;280(8):6511-9
15598662
Mol Endocrinol. 2006 May;20(5):1048-60
16373395
J Biol Chem. 2005 Apr 8;280(14):13600-5
15691842
Annu Rev Biochem. 1994;63:451-86
7979245
Nature. 2005 Jan 20;433(7023):278-85
15662416
Endocr Rev. 2007 Dec;28(7):778-808
17940184
J Biol Chem. 2002 Jul 26;277(30):26761-8
12004059
Oncogene. 2005 Jan 13;24(3):431-44
15558026
Proc Natl Acad Sci U S A. 2005 Apr 5;102(14):5156-61
15790678
BMC Genomics. 2008 Oct 11;9:476
18847501
Prostate. 2004 Jun 1;59(4):426-35
15065091
Science. 2007 May 11;316(5826):860-6
17495164
J Biol Chem. 2005 Oct 28;280(43):36355-63
16120611
J Biol Chem. 2002 Jan 18;277(3):2207-15
11714699
Oncogene. 2006 Nov 16;25(54):7212-23
16732317
J Biol Chem. 2008 Nov 28;283(48):33199-210
18801742
Oncogene. 2004 Sep 30;23(45):7561-70
15334066
Oncogene. 1998 Nov 12;17(19):2473-84
9824158
Endocr Relat Cancer. 2006 Dec;13(4):979-94
17158750
J Mol Med (Berl). 2008 May;86(5):597-608
18368381
Cancer Res. 2005 Oct 1;65(19):8575-8
16204018
J Mol Biol. 2009 Jan 16;385(2):446-56
19013180
Mol Endocrinol. 1997 Feb;11(2):148-61
9013762
J Biol Chem. 2007 Mar 9;282(10):7329-38
17202144
Virology. 2007 Jul 5;363(2):454-61
17335866
Mol Endocrinol. 2009 Feb;23(2):213-25
19074551
J Biol Chem. 2008 Oct 24;283(43):29341-54
18682402
Mol Cell Biol. 2001 Sep;21(18):6091-101
11509652
J Biol Chem. 2009 May 8;284(19):12633-44
19244234
Mol Cell. 2002 Mar;9(3):601-10
11931767
J Biol Chem. 2009 Jul 24;284(30):19856-66
19491398
Endocr Rev. 2002 Apr;23(2):175-200
11943742
J Cell Biol. 2000 Aug 21;150(4):873-80
10953010
Nat Rev Cancer. 2001 Oct;1(1):34-45
11900250