The beneficial effect of n-3 polyunsaturated fatty acids on doxorubicin-induced chronic heart failure in rats.
This study was designed to assess the effects of dietary supplementation with n-3 polyunsaturated fatty acids (PUFA) from fish oil on the response of doxorubicin-induced chronic heart failure in rats. Male Sprague-Dawley rats were treated daily for 8 weeks with normal saline or n-3 PUFA intragastrically after induction of myocardial injury by intraperitoneal injection of doxorubicin 2 mg/kg once weekly for 8 weeks. Cardiac function was assessed by echocardiography. The cytoprotective role of n-3 PUFA against doxorubicin-induced myocardial injury was demonstrated by light microscopy, and serum cytokines (tumour necrosis factor-alpha and interleukin-10) were analysed by enzyme-linked immunosorbent assay. Doxorubicin induced death, alterations in echocardiography parameters and histological damage, all of which are features that characterize heart failure. There were significant differences between the doxorubicin-induced heart failure group and the n-3 PUFA-treated group in terms of echocardiography parameters and cytokine changes. Thus, dietary supplementation with n-3 PUFA attenuated doxorubicin-induced cardiac dysfunction, an effect that might be associated with recovery from an imbalance of the cytokine network.
Teng
L L
LL
Department of Geratology, Shanghai East Hospital, Tongji University, Shanghai, China.
Shao
L
L
Zhao
Y T
YT
Yu
X
X
Zhang
D F
DF
Zhang
H
H
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000633:toxicity*; D002908:Chronic Disease; D004195:Disease Models, Animal; D004317:Doxorubicin / Q000633:toxicity*; D004452:Echocardiography; D015525:Fatty Acids, Omega-3 / Q000494:pharmacology*; D006321:Heart / Q000187:drug effects; D006333:Heart Failure / Q000097:blood / Q000503:physiopathology / Q000517:prevention & control*; D016753:Interleukin-10 / Q000097:blood; D008297:Male; D009206:Myocardium / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha / Q000097:blood; D016276:Ventricular Function / Q000187:drug effects / Q000502:physiology
D000903:Antibiotics, Antineoplastic; D015525:Fatty Acids, Omega-3; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D004317:Doxorubicin