Pseudomonas seleniipraecipitatus sp. nov.: a selenite reducing γ-proteobacteria isolated from soil.
A Gram-negative, yellow pigmented bacterium designated strain CA5(T) that reduced selenite to elemental red selenium was isolated from soil. 16S rRNA gene sequence alignment identified the isolate as a novel Pseudomonas species with P. argentinensis, P. flavescens, and P. straminea as its closest relatives. Sequence alignments show that the 16S rRNA gene of strain CA5(T) differed from that of P. argentinensis, P. flavescens, and P. straminea by 1.3, 1.5, and 1.7%, respectively. The G+C content was 62.8 mol%, similar to the 62.7-63 mol% reported for P. flavescens but slightly higher than the 62.5-62.6 mol% of P. straminea and significantly higher than the 57.5-58.0 mol% of P. argentinensis. The major cellular fatty acids present in the CA5(T) strain were C₁₈:₁ ω7c (41.1%), C₁₆:₁ ω6c and C₁₆:₁ ω7c (25.7%), C₁₆:₁ (12.0%), C₁₂:₀ (8.0%), C₁₂:₀ 3-OH (4.4%), and C₁₀:₀ 3-OH (2.9%). The cellular fatty acid profile, GC content, phenotypic properties, and biochemical characteristics were consistent with its placement within the genus Pseudomonas. The name P. seleniipraecipitatus is proposed for these bacteria.
Hunter
William J
WJ
USDA-ARS, 2150-D Centre Avenue, Fort Collins, CO, 80526-8119, USA, william.hunter@ars.usda.gov.
Manter
Daniel K
DK
D001482:Base Composition; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010802:Phylogeny; D011549:Pseudomonas / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D018038:Sodium Selenite / Q000378:metabolism*; D012988:Soil Microbiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D018038:Sodium Selenite
Proc Natl Acad Sci U S A. 2004 Jul 27;101(30):11030-5
15258291
Int J Syst Bacteriol. 1994 Jul;44(3):410-5
7520732
FEMS Microbiol Rev. 1997 Jul;20(3-4):503-16
9299717
J Lab Clin Med. 1954 Aug;44(2):301-7
13184240
Appl Environ Microbiol. 1998 Feb;64(2):795-9
9464425
Environ Microbiol. 2000 Feb;2(1):39-50
11243261
Mol Biol Evol. 1987 Jul;4(4):406-25
3447015
Int J Syst Bacteriol. 1997 Oct;47(4):1145-56
9336922
Extremophiles. 2005 Feb;9(1):75-9
15351875
Curr Microbiol. 2009 May;58(5):493-8
19189180
Mol Biol Evol. 2007 Aug;24(8):1596-9
17488738
Int J Syst Evol Microbiol. 2005 May;55(Pt 3):1107-1112
15879241
Int J Syst Evol Microbiol. 2000 Jul;50 Pt 4:1513-1519
10939658
Environ Microbiol. 2002 Nov;4(11):770-3
12460286
J Mol Evol. 1994 Mar;38(3):300-4
8006997
Int J Syst Evol Microbiol. 2006 Nov;56(Pt 11):2593-2595
17082397
Appl Environ Microbiol. 1982 Oct;44(4):992-3
6184019