title: Adducins regulate remodeling of apical junctions in human epithelial cells.
abstract: Epithelial adherens junctions (AJs) and tight junctions (TJs) are dynamic structures that readily undergo disintegration and reassembly. Remodeling of the AJs and TJs depends on the orchestrated dynamics of the plasma membrane with its underlying F-actin cytoskeleton, and the membrane-cytoskeleton interface may play a key role in junctional regulation. Spectrin-adducin-ankyrin complexes link membranes to the actin cytoskeleton where adducins mediate specrtrin-actin interactions. This study elucidates roles of adducins in the remodeling of epithelial junctions in human SK-CO15 colonic and HPAF-II pancreatic epithelial cell monolayers. These cells expressed the α and γ isoforms of adducin that positively regulated each others protein level and colocalized with E-cadherin and β-catenin at mature, internalized and newly assembled AJs. Small interfering RNA-mediated down-regulation of α- or γ-adducin expression significantly attenuated calcium-dependent AJ and TJ assembly and accelerated junctional disassembly triggered by activation of protein kinase C. Two mechanisms were found to mediate the impaired AJ and TJ assembly in adducin-depleted cells. One mechanism involved diminished expression and junctional recruitment of βII-spectrin, and the other mechanism involved the decrease in the amount of cellular F-actin and impaired assembly of perijunctional actin bundles. These findings suggest novel roles for adducins in stabilization of epithelial junctions and regulation of junctional remodeling.
authors.affiliation: Department of Medicine, University of Rochester, Rochester, NY 14642, USA.
authors.forename: Nayden G
authors.initials: NG
authors.lastname: Naydenov
authors.forename: Andrei I
authors.initials: AI
authors.lastname: Ivanov
mesh_terms: D000199:Actins / Q000378:metabolism; D022005:Adherens Junctions / Q000201:enzymology / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D002148:Calmodulin-Binding Proteins / Q000378:metabolism*; D002460:Cell Line; D016764:Cell Polarity; D003599:Cytoskeleton / Q000378:metabolism; D015536:Down-Regulation; D004789:Enzyme Activation; D004847:Epithelial Cells / Q000166:cytology* / Q000201:enzymology / Q000378:metabolism*; D055785:Gene Knockdown Techniques; D006801:Humans; D007422:Intestines / Q000166:cytology; D010179:Pancreas / Q000166:cytology; D020033:Protein Isoforms / Q000378:metabolism; D011493:Protein Kinase C / Q000378:metabolism; D021381:Protein Transport; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism; D013049:Spectrin / Q000378:metabolism; D019108:Tight Junctions / Q000201:enzymology / Q000378:metabolism*; D000099291:Adducins
chemical_list: D000199:Actins; D002118:Calcium; D002148:Calmodulin-Binding Proteins; D020033:Protein Isoforms; D011493:Protein Kinase C; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D013049:Spectrin; D000099291:Adducins; C102021:ADD3 protein, human; C452439:SPTBN1 protein, human
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