title: Cardiotoxic effects of Loxosceles intermedia spider venom and the recombinant venom toxin rLiD1.
abstract: Loxosceles spider bites cause many human injuries worldwide. Injections in mice of whole Loxosceles (L.) intermedia venom or a recombinant toxin (rLiD1) produce systemic symptoms similar to those detected in envenomed humans. This animal model was used to characterize the effects of Loxosceles intermedia venom in cardiac tissues. L. intermedia antigens were detected by ELISA in kidney, heart, lung and liver of experimentally envenomed mice. In addition, rLiD1 binding to cardiomyocytes was demonstrated by immunofluorescence and confocal microscopy. Furthermore, isolated perfused heart preparations and ventricular cardiomyocytes from envenomed mice showed heart function impairment, and a significant increase of I(Ca,L) density and intracellular Ca(2+) transients, respectively. Thus, L. intermedia spider venom, as shown through the use of the recombinant toxin rLiD1, causes cardiotoxic effects and a protein from the sphingomyelinase D family plays a key role in heart dysfunction. Thus, L. intermedia spider venom and the Loxtox rLiD1 play a key role in heart dysfunction.
authors.affiliation: Biochemistry Departament, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, CP: 486, CEP: 30161-970, Belo Horizonte, Brazil.
authors.forename: Camila
authors.initials: C
authors.lastname: Dias-Lopes
authors.forename: Liza
authors.initials: L
authors.lastname: Felicori
authors.forename: Gabriela
authors.initials: G
authors.lastname: Guimarães
authors.forename: Eneas R M
authors.initials: ER
authors.lastname: Gomes
authors.forename: Danilo
authors.initials: D
authors.lastname: Roman-Campos
authors.forename: Hugo
authors.initials: H
authors.lastname: Duarte
authors.forename: Denis
authors.initials: D
authors.lastname: Damasceno
authors.forename: Marilia
authors.initials: M
authors.lastname: Martins
authors.forename: Evanguedes
authors.initials: E
authors.lastname: Kalapothakis
authors.forename: Alvair P
authors.initials: AP
authors.lastname: Almeida
authors.forename: Claude
authors.initials: C
authors.lastname: Granier
authors.forename: Jader S
authors.initials: JS
authors.lastname: Cruz
authors.forename: Silvia
authors.initials: S
authors.lastname: Guatimosim
authors.forename: Carlos
authors.initials: C
authors.lastname: Chávez-Olórtegui
mesh_terms: D000818:Animals; D000941:Antigens / Q000032:analysis; D002118:Calcium / Q000378:metabolism; D054715:Cardiotoxins / Q000276:immunology / Q000302:isolation & purification / Q000633:toxicity*; D002478:Cells, Cultured; D003402:Creatine Kinase / Q000097:blood; D052279:Creatine Kinase, MB Form / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006321:Heart / Q000187:drug effects*; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009206:Myocardium / Q000473:pathology*; D032383:Myocytes, Cardiac / Q000187:drug effects; D018408:Patch-Clamp Techniques; D010727:Phosphoric Diester Hydrolases / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology / Q000633:toxicity*; D011993:Recombinant Fusion Proteins; D013111:Spider Venoms / Q000276:immunology / Q000302:isolation & purification / Q000633:toxicity*
chemical_list: D000941:Antigens; D054715:Cardiotoxins; D011993:Recombinant Fusion Proteins; D013111:Spider Venoms; C055161:loxosceles venom; D003402:Creatine Kinase; D052279:Creatine Kinase, MB Form; D010727:Phosphoric Diester Hydrolases; C055160:sphingomyelin phosphodiesterase D; D002118:Calcium