title: Isothermal sensitive detection of microRNA using an autonomous DNA machine recycling output as input.
abstract: An autonomous DNA machine recycling the output as the input for isothermal, sensitive, and specific detection of miRNAs has been developed. This machine shows considerably high signal amplification efficiency (∼1000-fold) and thus a low detection limit (∼20 amol). The machine also shows high specificity, discriminating 50 amol of synthetic miRNA from 100-fold larger amounts of its family member and from 100 ng of unrelated total RNAs. Moreover, it is available for practically detecting natural miRNAs in total RNAs.
authors.affiliation: Senior Research Fellow Center, Ehime University, Matsuyama, Ehime, Japan. a-ogawa@ccr.ehime-u.ac.jp
authors.forename: Atsushi
authors.initials: A
authors.lastname: Ogawa
mesh_terms: D000818:Animals; D001483:Base Sequence; D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000378:metabolism; D057230:Limit of Detection; D051379:Mice; D035683:MicroRNAs / Q000032:analysis* / Q000378:metabolism; D021141:Nucleic Acid Amplification Techniques / Q000379:methods; D013696:Temperature
chemical_list: D035683:MicroRNAs; D004247:DNA