title: Novel technology to provide an enriched therapeutic cell concentrate from bone marrow aspirate.
abstract: Current strategies to repair fractures rely on orthopaedic surgeons harvesting bone from one area of the body, typically pelvis and transferring it to the fracture site. The amount of tissue available is therefore limited, requiring a second surgical procedure and often causing the patient long term pain. An alternative approach is utilise therapeutic cells contained within bone marrow aspirate during the primary procedure. The number of therapeutic cells within a fresh aspirate is insufficient to provide clinically acceptable bone healing in a timescale that is satisfactory to the surgeon and the patient. Therefore methods to efficiently concentrate bone marrow in the clinical setting are required. Centrifugation is the current method of choice but has limitations in that it requires large capital equipment, servicing and there are potential issues of tissue contamination. We have developed a novel, acoustically-assisted filtration device that addresses these limitations, delivering a concentrated bone marrow in a point of care, single use, fully disposable, compact device. An additional advantage is that the level of concentration required can be specified by the end user. The resulting bone marrow concentrate has been characterised in terms of cell number, viability and osteogenic potential using flow cytometry and alkaline phosphatase assay. When compared to recent clinical studies using bone marrow to repair non-union fractures, the findings from our work suggest that the bone marrow concentrate is likely to be highly therapeutic and clinically efficacious as a bone fracture repair strategy. A product concept for use in the clinical setting is presented.
authors.affiliation: Smith & Nephew, Biologics and Spine, York Science Park, Heslington, York YO10 5DF, UK.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Ridgway
authors.forename: Andrew
authors.initials: A
authors.lastname: Butcher
authors.forename: Pei-Sheng
authors.initials: PS
authors.lastname: Chen
authors.forename: Alan
authors.initials: A
authors.lastname: Horner
authors.forename: Stephen
authors.initials: S
authors.lastname: Curran
mesh_terms: D001854:Bone Marrow Cells / Q000166:cytology*; D005374:Filtration / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D008567:Membranes, Artificial; D016062:Porosity; D013499:Surface Properties; D014618:Vacuum
chemical_list: D008567:Membranes, Artificial