[Cardioprotection of adenosine treatment in cadaver donor rats with warm ischemia].
OBJECTIVE
To determine the cardioprotection of adenosine treatment in cadaver donor rats with warm ischemia.


METHODS
Rats were randomly divided into 7 groups. Group C was a control group. Group I(5), I(15), and I(30): The donor hearts from the cadaver rats that died of acute hemorrhagic shock and within warm ischemic durations of 5, 15, and 30 min, respectively, and the donor hearts were perfused with STH-1 for 30 min before cervical heterotopic heart transplantation. Group A(5), A(15), and A(30) were adenosine treatment groups and the donor hearts were obtained as mentioned above and perfused with STH-1 plus adenosine (adenosine concentration 1.2 mmol/L) before cervical heterotopic heart transplantation. We detected the change of the grafts including ultrastructure, the level of ATP, SOD, and MDA, NF-kappaB mRNA, TNF-alpha, and IL-6 24 h after the transplantation.


RESULTS
At the same time point, compared with Group I(5), I(15), and I(30), Group A(5), A(15), and A(30) showed increased ATP and SOD in the myocardial tissues, and decreased MDA, NF-kappaB mRNA, TNF-alpha, and IL-6 (P<0.05).


CONCLUSIONS
Adenosine treatment can protect the graft in cadaver donor rats with warm ischemia. The mechanism may improve the myocardial energy metabolism, attenuate lipid peroxidation injury, suppress the expression of NF-kappaB mRNA, and decrease the inflammatory damage of TNF-alpha and IL-6.
Wu
Dongkai
D
Department of Cardiothoracic Surgery, Xiangya Hospital, Central South University, Changsha 410008, China.
Chen
Shengxi
S
Luo
Wanjun
W
Jiang
Haihe
H
D000241:Adenosine / Q000494:pharmacology*; D000818:Animals; D002316:Cardiotonic Agents / Q000494:pharmacology*; D006321:Heart / Q000187:drug effects*; D016027:Heart Transplantation; D008297:Male; D015428:Myocardial Reperfusion Injury / Q000517:prevention & control; D009926:Organ Preservation / Q000379:methods*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D052096:Warm Ischemia / Q000379:methods*
D002316:Cardiotonic Agents; D000241:Adenosine