title: In vivo imaging and therapeutic treatments in an orthotopic mouse model of ovarian cancer.
abstract: Human cancer and response to therapy is better represented in orthotopic animal models. This paper describes the development of an orthotopic mouse model of ovarian cancer, treatment of cancer via oral delivery of drugs, and monitoring of tumor cell behavior in response to drug treatment in real time using in vivo imaging system. In this orthotopic model, ovarian tumor cells expressing luciferase are applied topically by injecting them directly into the mouse bursa where each ovary is enclosed. Upon injection of D-luciferin, a substrate of firefly luciferase, luciferase-expressing cells generate bioluminescence signals. This signal is detected by the in vivo imaging system and allows for a non-invasive means of monitoring tumor growth, distribution, and regression in individual animals. Drug administration via oral gavage allows for a maximum dosing volume of 10 mL/kg body weight to be delivered directly to the stomach and closely resembles delivery of drugs in clinical treatments. Therefore, techniques described here, development of an orthotopic mouse model of ovarian cancer, oral delivery of drugs, and in vivo imaging, are useful for better understanding of human ovarian cancer and treatment and will improve targeting this disease.
authors.affiliation: Department of Medical Oncology, Women's Cancer Program, Fox Chase Cancer Center, USA.
authors.forename: Alexis B
authors.initials: AB
authors.lastname: Cordero
authors.forename: Youngjoo
authors.initials: Y
authors.lastname: Kwon
authors.forename: Xiang
authors.initials: X
authors.lastname: Hua
authors.forename: Andrew K
authors.initials: AK
authors.lastname: Godwin
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D003952:Diagnostic Imaging / Q000379:methods*; D005260:Female; D006801:Humans; D008156:Luciferases / Q000032:analysis / Q000096:biosynthesis; D051379:Mice; D009368:Neoplasm Transplantation; D010051:Ovarian Neoplasms / Q000188:drug therapy / Q000201:enzymology / Q000473:pathology*; D014183:Transplantation, Heterologous; D023041:Xenograft Model Antitumor Assays / Q000379:methods*
chemical_list: D008156:Luciferases
references.citation: Cancer Metastasis Rev. 1998-1999;17(3):279-84
references.pmid: 10352881
references.citation: Proc Am Thorac Soc. 2005;2(6):537-40, 511-2
references.pmid: 16352761
references.citation: Nat Rev Cancer. 2009 Mar;9(3):167-81
references.pmid: 19238149
references.citation: Proc Natl Acad Sci U S A. 2009 Aug 18;106(33):14004-9
references.pmid: 19666490
references.citation: CA Cancer J Clin. 2009 Jul-Aug;59(4):225-49
references.pmid: 19474385
references.citation: Curr Protoc Pharmacol. 2009 Jun 1;45:14.12.1-14.12.26
references.pmid: 20634901
references.citation: Gynecol Oncol. 2008 Feb;108(2):385-94
references.pmid: 18036641
references.citation: Mol Oncol. 2009 Apr;3(2):97-137
references.pmid: 19383374
references.citation: Mol Ther. 2004 Dec;10(6):1032-42
references.pmid: 15564135
references.citation: Cancer Treat Res. 2009;149:353-91
references.pmid: 19763445
references.citation: Eur J Cancer. 2004 Apr;40(6):852-7
references.pmid: 15120041