title: The effects of rapamycin on lens epithelial cell proliferation, migration, and matrix formation: an in vitro study.
abstract: OBJECTIVE
  The objective of the present study was to investigate the efficacy of rapamycin on rabbit lens epithelial cell proliferation, migration, and secretion of extracellular matrix fibronectin (Fn).
  
  
  METHODS
  Rabbit lens epithelium cells (rLECs) were isolated from 1 month old rabbit. rLECs were either cultured for 24, 48, or 72 h with different doses of rapamycin (0.1, 1, and 10 ng/ml). The proliferation kinetics, proliferating cell nuclear antigen (PCNA) expression, and basic fibroblast growth factor (bFGF)-induced migration of rLEC was determined by methyl thiazol tetrazolium (MTT) assay, western blotting and transwell chamber assay, respectively. The effect of rapamycin on the synthesis of Fn was examined via immunofluorescence.
  
  
  RESULTS
  Rapamycin significantly inhibited rLEC proliferation and PCNA protein expression when administered doses and time periods except for 0.1 ng/ml for 24 h. bFGF-induced migration rLECs was inhibited by pretreatment with rapamycin for 48 h. Extracellular matrix Fn formation of rLECs was also reduced by rapamycin.
  
  
  CONCLUSIONS
  In our study, rapamycin strongly inhibited rLEC proliferation, bFGF-induced migration, and extracellular matrix Fn formation. Thus, rapamycin may have a potential inhibition of posterior capsule opacification (PCO) and needs further study.
authors.affiliation: Department of Ophthalmology, The 1st Affiliated Hospital, Harbin Medical University, Harbin, PR China.
authors.forename: Hongling
authors.initials: H
authors.lastname: Liu
authors.forename: Guangzhong
authors.initials: G
authors.lastname: Feng
authors.forename: Lan
authors.initials: L
authors.lastname: Wu
authors.forename: Shaoying
authors.initials: S
authors.lastname: Fu
authors.forename: Ping
authors.initials: P
authors.lastname: Liu
authors.forename: Wei
authors.initials: W
authors.lastname: Yang
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D004847:Epithelial Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D005109:Extracellular Matrix / Q000187:drug effects* / Q000378:metabolism*; D005353:Fibronectins / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D007908:Lens, Crystalline / Q000166:cytology*; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D011817:Rabbits; D020123:Sirolimus / Q000494:pharmacology*
chemical_list: D005353:Fibronectins; D018809:Proliferating Cell Nuclear Antigen; D020123:Sirolimus
references.citation: J Clin Invest. 1996 Nov 15;98(10):2277-83
references.pmid: 8941644
references.citation: Ophthalmologe. 1994 Feb;91(1):98-102
references.pmid: 8173260
references.citation: J Immunol Methods. 1983 Dec 16;65(1-2):55-63
references.pmid: 6606682
references.citation: Exp Mol Med. 2006 Apr 30;38(2):173-9
references.pmid: 16672771
references.citation: Tissue Cell. 1986;18(1):83-97
references.pmid: 3515629
references.citation: Circulation. 2001 Sep 4;104(10):1188-93
references.pmid: 11535578
references.citation: J Cell Biol. 1988 Apr;106(4):1365-73
references.pmid: 3360855
references.citation: Graefes Arch Clin Exp Ophthalmol. 2009 Jun;247(6):801-7
references.pmid: 19066932
references.citation: N Engl J Med. 2003 Oct 2;349(14):1315-23
references.pmid: 14523139
references.citation: Graefes Arch Clin Exp Ophthalmol. 2003 Apr;241(4):309-13
references.pmid: 12719992
references.citation: Exp Eye Res. 1992 Mar;54(3):393-401
references.pmid: 1521568
references.citation: Int J Dev Biol. 2004;48(8-9):857-65
references.pmid: 15558477
references.citation: J Cell Physiol. 1982 Nov;113(2):273-80
references.pmid: 7174730
references.citation: Br J Ophthalmol. 2000 Mar;84(3):332-6
references.pmid: 10684849
references.citation: J Fr Ophtalmol. 1997;20(9):664-70
references.pmid: 9587577
references.citation: J Cell Biol. 1986 Jan;102(1):179-88
references.pmid: 3941152
references.citation: Invest Ophthalmol Vis Sci. 1995 Oct;36(11):2304-12
references.pmid: 7558725
references.citation: Mol Vis. 2006 Oct 26;12:1233-42
references.pmid: 17110906
references.citation: Circulation. 2003 Feb 11;107(5):777-84
references.pmid: 12578884
references.citation: J Cell Physiol. 1985 Dec;125(3):393-402
references.pmid: 3905828
references.citation: Differentiation. 1984;28(1):53-61
references.pmid: 6394411
references.citation: Surv Ophthalmol. 1992 Sep-Oct;37(2):73-116
references.pmid: 1455302
references.citation: J Cataract Refract Surg. 1996;22 Suppl 1:835-40
references.pmid: 9279681
references.citation: Ophthalmology. 1983 Dec;90(12):1548-53
references.pmid: 6677855