title: Novel use of tryptose sulfite cycloserine egg yolk agar for isolation of Clostridium perfringens during an outbreak of necrotizing enterocolitis in a neonatal unit.
abstract: Clostridium perfringens has been associated with necrotizing enterocolitis (NEC), which is a serious disease of neonates. Our study describes the novel use of selective tryptose sulfite cycloserine with egg yolk agar (TSC-EYA) during a nursery outbreak. This medium provides a rapid, sensitive, and accurate presumptive identification of C. perfringens.
authors.affiliation: Department of Infectious Diseases, Southern Health, Monash Medical Centre, Clayton, Melbourne, Victoria, Australia. despina.kotsanas@southernhealth.org.au
authors.forename: Despina
authors.initials: D
authors.lastname: Kotsanas
authors.forename: Jolene A
authors.initials: JA
authors.lastname: Carson
authors.forename: Milena M
authors.initials: MM
authors.lastname: Awad
authors.forename: Dena
authors.initials: D
authors.lastname: Lyras
authors.forename: Julian I
authors.initials: JI
authors.lastname: Rood
authors.forename: Grant A
authors.initials: GA
authors.lastname: Jenkin
authors.forename: Rhonda L
authors.initials: RL
authors.lastname: Stuart
authors.forename: Tony M
authors.initials: TM
authors.lastname: Korman
mesh_terms: D000362:Agar; D001431:Bacteriological Techniques / Q000379:methods*; D003015:Clostridium Infections / Q000175:diagnosis* / Q000453:epidemiology / Q000382:microbiology; D003016:Clostridium perfringens / Q000302:isolation & purification*; D003470:Culture Media / Q000737:chemistry*; D003523:Cycloserine / Q000378:metabolism; D004196:Disease Outbreaks; D004530:Egg Yolk / Q000378:metabolism; D020345:Enterocolitis, Necrotizing / Q000453:epidemiology* / Q000382:microbiology*; D006801:Humans; D007231:Infant, Newborn; D009930:Organic Chemicals / Q000378:metabolism; D012680:Sensitivity and Specificity; D013447:Sulfites / Q000378:metabolism
chemical_list: D003470:Culture Media; D009930:Organic Chemicals; D013447:Sulfites; C046666:tryptose; D000362:Agar; D003523:Cycloserine
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