Injury-induced CRMP4 expression in adult sensory neurons; a possible target gene for ciliary neurotrophic factor.
Neurotrophic cytokines, such as ciliary neurotrophic factor (CNTF) play an important role in the development and regeneration of the nervous system. In the present study, we screened gene expression induced by CNTF in adult dorsal root ganglion (DRG) neurons using the Illumina microarray. We found that the expression of both short and long forms of collapsin response-mediator protein 4 (CRMP4) was increased in cultured primary sensory neurons by CNTF. In addition, sciatic nerve injury induced the expression of CRMP4 mRNA and protein in DRG neurons. Finally, the increased CRMP4 protein was transported into peripheral axons following nerve injury. These findings indicate that CRMP4 may be a target gene for CNTF in the regenerative axon growth of DRG neurons after injury.
Jang
So Young
SY
Department of Physiology, Mitochondria Hub Regulation Center, College of Medicine, Dong-A University, South Korea.
Shin
Yoon Kyung
YK
Jung
Junyang
J
Lee
Sang Hwa
SH
Seo
Su-Yeong
SY
Suh
Duk Joon
DJ
Park
Hwan Tae
HT
D000818:Animals; D001369:Axons / Q000378:metabolism; D002478:Cells, Cultured; D020934:Ciliary Neurotrophic Factor / Q000494:pharmacology / Q000502:physiology*; D005727:Ganglia, Spinal / Q000166:cytology / Q000378:metabolism*; D020869:Gene Expression Profiling; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D021381:Protein Transport; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000293:injuries / Q000378:metabolism; D011984:Sensory Receptor Cells / Q000378:metabolism*
D020934:Ciliary Neurotrophic Factor; C449760:Dpysl3 protein, rat; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger