Sulbutiamine counteracts trophic factor deprivation induced apoptotic cell death in transformed retinal ganglion cells.
Sulbutiamine is a highly lipid soluble synthetic analogue of vitamin B(1) and is used clinically for the treatment of asthenia. The aim of our study was to demonstrate whether sulbutiamine is able to attenuate trophic factor deprivation induced cell death to transformed retinal ganglion cells (RGC-5). Cells were subjected to serum deprivation for defined periods and sulbutiamine at different concentrations was added to the cultures. Various procedures (e.g. cell viability assays, apoptosis assay, reactive oxygen species analysis, Western blot analysis, flow cytometric analysis, glutathione (GSH) and glutathione-S-transferase (GST) measurement) were used to demonstrate the effect of sulbutiamine. Sulbutiamine dose-dependently attenuated apoptotic cell death induced by serum deprivation and stimulated GSH and GST activity. Moreover, sulbutiamine decreased the expression of cleaved caspase-3 and AIF. This study demonstrates for the first time that sulbutiamine is able to attenuate trophic factor deprivation induced apoptotic cell death in neuronal cells in culture.
Kang
Kui Dong
KD
Department of Ophthalmology, Oxford Eye Hospital, University of Oxford, Headington, Oxford, UK.
Majid
Aman Shah Abdul
AS
Kim
Kyung-A
KA
Kang
Kyungsu
K
Ahn
Hong Ryul
HR
Nho
Chu Won
CW
Jung
Sang Hoon
SH
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D051033:Apoptosis Inducing Factor / Q000378:metabolism; D053148:Caspase 3 / Q000378:metabolism; D002461:Cell Line, Transformed; D002470:Cell Survival / Q000187:drug effects; D016895:Culture Media, Serum-Free / Q000494:pharmacology; D005978:Glutathione / Q000378:metabolism; D051379:Mice; D017382:Reactive Oxygen Species / Q000378:metabolism; D012165:Retinal Ganglion Cells / Q000187:drug effects*; D013831:Thiamine / Q000031:analogs & derivatives* / Q000494:pharmacology
D051033:Apoptosis Inducing Factor; D016895:Culture Media, Serum-Free; C494830:AIFM1 protein, mouse; D017382:Reactive Oxygen Species; C031331:sulbutiamine; D053148:Caspase 3; D005978:Glutathione; D013831:Thiamine
Intern Med. 1997 Jul;36(7):532
9240508
Photosynth Res. 2005 Dec;86(3):435-57
16315075
Curr Opin Ophthalmol. 2002 Apr;13(2):61-7
11880717
Am J Physiol Lung Cell Mol Physiol. 2007 Jun;292(6):L1515-25
17337508
Mol Pharmacol. 2004 Nov;66(5):1113-22
15304547
Invest Ophthalmol Vis Sci. 1999 Oct;40(11):2660-7
10509663
Free Radic Biol Med. 2008 Aug 15;45(4):367-76
18489911
Brain Res Mol Brain Res. 2001 Jan 31;86(1-2):1-12
11165366
Am J Manag Care. 2008 Feb;14(1 Suppl):S15-9
18284311
Invest Ophthalmol Vis Sci. 2005 Mar;46(3):877-83
15728543
Prog Retin Eye Res. 2006 Sep;25(5):490-513
16962364
Vision Res. 2007 Apr;47(9):1191-201
17306853
Basic Clin Pharmacol Toxicol. 2008 Nov;103(5):482-6
18715237
J Biol Chem. 1994 May 20;269(20):14379-85
8182042
Neurosci Lett. 2000 Sep 29;292(1):49-53
10996447
Pharmacol Biochem Behav. 1985 Aug;23(2):195-8
4059305
Cell Mol Life Sci. 2003 Jul;60(7):1477-88
12943234
J Immunol Methods. 1983 Dec 16;65(1-2):55-63
6606682
Brain Res. 2007 Jul 16;1158:158-63
17555724
Surv Ophthalmol. 2008 Sep-Oct;53(5):479-505
18929760
Mutat Res. 2006 Mar;612(2):105-14
16413223
World J Biol Psychiatry. 2006;7(3):183-5
16861144
Arch Ophthalmol. 2003 Jan;121(1):48-56
12523884
Brain Res. 1994 Jul 25;652(1):157-60
7953714
Annu Rev Biochem. 1983;52:711-60
6137189
Prog Retin Eye Res. 2004 Jan;23(1):91-147
14766318
Brain Res. 2009 Jan 28;1251:269-75
19056360
Prog Retin Eye Res. 1999 Jan;18(1):39-57
9920498
Invest Ophthalmol Vis Sci. 2005 Apr;46(4):1330-8
15790899
Ann Ophthalmol. 1979 Jul;11(7):1095-1100
485004
Ann Pharmacother. 1995 Dec;29(12):1263-73
8672832
Biochem Pharmacol. 1990 Dec 1;40(11):2557-60
2268373
J Assoc Physicians India. 2003 Sep;51:891-5
14710977
Physiol Rev. 2002 Jan;82(1):47-95
11773609
Exp Eye Res. 2007 Mar;84(3):389-99
17196589
Urologiia. 2005 Jan-Feb;(1):32-5
15776829
Methods Enzymol. 1995;251:203-12
7651198
Invest Ophthalmol Vis Sci. 2010 Jan;51(1):284-94
19710416
Am J Physiol. 1989 Oct;257(4 Pt 1):L163-73
2572174
Brain Res. 2007 Jul 23;1159:40-53
17573045
Invest Ophthalmol Vis Sci. 2009 Sep;50(9):4267-72
19443730
Can J Ophthalmol. 2007 Jun;42(3):425-38
17508040
Encephale. 2007 Mar-Apr;33(2):211-5
17675917
Drugs Today (Barc). 1999 Mar;35(3):187-92
12973384
Cancer Lett. 2003 Dec 30;202(2):125-9
14643441
Proc Natl Acad Sci U S A. 1981 Sep;78(9):5886-9
6272323
Life Sci. 2009 Jan 2;84(1-2):28-32
18992261
Chem Biol Interact. 2007 Jan 30;165(2):146-54
17207784
Curr Opin Ophthalmol. 1999 Apr;10(2):82-92
10537768
Brain Res. 2005 Aug 16;1053(1-2):185-94
16051195
J Neurol Neurosurg Psychiatry. 2008 Jul;79(7):846-7
18559466