title: Lack of protection of pre-immunization with saliva of long-term colonized Phlebotomus papatasi against experimental challenge with Leishmania major and saliva of wild-caught P. papatasi.
abstract: Immunity to saliva of Phlebotomus papatasi protects against Leishmania major infection as determined by co-inoculation of parasites with salivary gland homogenates (SGHs) of this vector. These results were obtained with long-term colonized female P. papatasi. We investigated the effect of pre-immunization with SGH of long-term colonized P. papatasi against L. major infection co-inoculated with SGH of wild-caught P. papatasi. Our results showed that pre-exposure to SGH of long-term, colonized P. papatasi do not confer protection against infection with L. major co-inoculated with SGH of wild-caught P. papatasi. These preliminary results strongly suggest that the effectiveness of a vector saliva-based vaccine derived from colonized sand fly populations may be affected by inconsistent immune response after natural exposure.
authors.affiliation: Laboratory of Vector Ecology, Laboratory of Epidemiology and Ecology of Parasites, and Laboratory of Immuno-Pathology, Vaccinology, and Molecular Genetics, Institut Pasteur de Tunis, Tunis, Tunisia.
authors.forename: Sami Ben Hadj
authors.initials: SB
authors.lastname: Ahmed
authors.forename: Belhassen
authors.initials: B
authors.lastname: Kaabi
authors.forename: Ifhem
authors.initials: I
authors.lastname: Chelbi
authors.forename: Mohamed
authors.initials: M
authors.lastname: Derbali
authors.forename: Saifedine
authors.initials: S
authors.lastname: Cherni
authors.forename: Dhafer
authors.initials: D
authors.lastname: Laouini
authors.forename: Elyes
authors.initials: E
authors.lastname: Zhioua
mesh_terms: D000818:Animals; D005260:Female; D018320:Leishmania major / Q000276:immunology*; D010691:Phlebotomus / Q000276:immunology*; D012463:Saliva
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