MiRNA editing.
RNA editing by A-to-I modification is a widespread mechanism in complex organisms that leads to the posttranscriptional alteration of protein coding as well as noncoding sequences. MiRNA transcripts have been recognized as a major target for RNA editing enzymes, and single-nucleotide changes through editing can impact the biogenesis of mature miRNAs, as well as the target specificity of the regulatory RNA. Bona fide A-to-I RNA editing events are validated experimentally through parallel analysis of genomic DNA and transcribed sequences of miRNA genes isolated from the same specimen through gene-specific amplification and sequencing of endogenous transcripts.
Dupuis
Dylan E
DE
Department of Biological Sciences, Lehigh University, Bethlehem, PA, USA.
Maas
Stefan
S
D000243:Adenosine Deaminase / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D008969:Molecular Sequence Data; D017393:RNA Editing; D012322:RNA Precursors / Q000235:genetics / Q000378:metabolism; D012323:RNA Processing, Post-Transcriptional; D016601:RNA-Binding Proteins; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000295:instrumentation / Q000379:methods
D035683:MicroRNAs; D012322:RNA Precursors; D016601:RNA-Binding Proteins; C567955:ADARB1 protein, human; D000243:Adenosine Deaminase