In vivo discrimination of type 3 secretion system-positive and -negative Pseudomonas aeruginosa via a caspase-1-dependent pathway.
Microbe-associated molecular patterns are recognized by Toll-like receptors of the innate immune system. This recognition enables a rapid response to potential pathogens but does not clearly provide a way for the innate immune system to discriminate between virulent and avirulent microbes. We find that pulmonary infection of mice with type 3 translocation-competent Pseudomonas aeruginosa triggers a rapid inflammatory response, while infection with isogenic translocation-deficient mutants does not. Discrimination between translocon-positive and -negative bacteria requires caspase-1 activity in bone marrow-derived cells and interleukin-1 receptor signaling. Thus, the activation of caspase-1 by bacteria expressing type 3 secretion systems allows for rapid recognition of bacteria expressing conserved functions associated with virulence.
Wangdi
Tamding
T
Department of Internal Medicine (Infectious Diseases), Yale University School of Medicine, New Haven, CT 06520, USA.
Mijares
Lilia A
LA
Kazmierczak
Barbara I
BI
D000818:Animals; D001426:Bacterial Proteins / Q000276:immunology / Q000378:metabolism*; D001854:Bone Marrow Cells / Q000276:immunology; D020170:Caspase 1 / Q000378:metabolism*; D004847:Epithelial Cells / Q000382:microbiology; D007113:Immunity, Innate; D008168:Lung / Q000276:immunology / Q000382:microbiology; D016676:Macrophages, Alveolar / Q000276:immunology / Q000382:microbiology; D051379:Mice; D008810:Mice, Inbred C57BL; D020556:Neutrophil Infiltration; D011552:Pseudomonas Infections / Q000201:enzymology / Q000276:immunology* / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000276:immunology / Q000378:metabolism / Q000472:pathogenicity*; D017472:Receptors, Interleukin-1 / Q000378:metabolism; D015398:Signal Transduction; D014132:Trachea / Q000166:cytology / Q000382:microbiology; D014774:Virulence
D001426:Bacterial Proteins; D017472:Receptors, Interleukin-1; D020170:Caspase 1
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