title: The analysis of café espresso using two-dimensional reversed phase-reversed phase high performance liquid chromatography with UV-absorbance and chemiluminescence detection.
abstract: In this study, an activity based screening technique combining two-dimensional liquid chromatography (2DHPLC) with UV-absorbance and chemiluminescence detection was applied to study "Ristretto", "Decaffeinatto" and "Volluto" espresso coffees. This technique, which coupled the separation power of 2DHPLC with the sensitivity and selectivity of the chemiluminescence detection, offers great potential for screening complex samples for antioxidant compounds. Detailed information regarding the complexity of the sample, and the variation between these three coffees could be obtained using this multidimensional-hyphenated method of analysis.
authors.affiliation: Australian Centre for Research on Separation Science, Nanoscale Organisation and Dynamics Group, and the School of Natural Sciences, University of Western Sydney, Parramatta, NSW 2050, Australia.
authors.forename: Mariam
authors.initials: M
authors.lastname: Mnatsakanyan
authors.forename: Paul G
authors.initials: PG
authors.lastname: Stevenson
authors.forename: Xavier A
authors.initials: XA
authors.lastname: Conlan
authors.forename: Paul S
authors.initials: PS
authors.lastname: Francis
authors.forename: Tiffany A
authors.initials: TA
authors.lastname: Goodie
authors.forename: Geoffrey P
authors.initials: GP
authors.lastname: McDermott
authors.forename: Neil W
authors.initials: NW
authors.lastname: Barnett
authors.forename: R Andrew
authors.initials: RA
authors.lastname: Shalliker
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D057230:Limit of Detection; D049449:Luminescence; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*