title: PNA-FISH on human sperm.
abstract: Peptide nucleic acids (PNAs) constitute a remarkable new class of synthetic nucleic acids analogs, based on peptide-like backbone. This structure gives PNAs the capacity to hybridize with high affinity and specificity to complementary RNA and DNA sequences. Over the last few years, the use of PNAs has proven its efficacy in cytogenetics for the rapid in situ identification of human chromosomes. Multicolour PNA-FISH protocols have been described and their adaptation to human spermatozoa has allowed the development of a new and fast procedure, which can advantageously be used for the assessment of aneuploidy in male gametes.
authors.affiliation: Service de Biologie de la Reproduction, Hôpital Arnaud de Villeneuve, CHRU Montpellier, Montpellier, France. f-pellestor@chu-montpellier.fr
authors.forename: Franck
authors.initials: F
authors.lastname: Pellestor
authors.forename: Cécile
authors.initials: C
authors.lastname: Monzo
authors.forename: Samir
authors.initials: S
authors.lastname: Hamamah
mesh_terms: D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism; D002875:Chromosomes / Q000235:genetics; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008297:Male; D008856:Microscopy, Fluorescence; D020135:Peptide Nucleic Acids / Q000235:genetics* / Q000378:metabolism; D013094:Spermatozoa / Q000166:cytology* / Q000378:metabolism
chemical_list: D020135:Peptide Nucleic Acids