A metabonomic approach to analyze the dexamethasone-induced cleft palate in mice.
Mice models are an important way to understand the relation between the fetus with cleft palate and changes of maternal biofluid. This paper aims to develop a metabonomics approach to analyze dexamethasone-induced cleft palate in pregnant C57BL/6J mice and to study the relationship between the change of endogenous small molecular metabolites in maternal plasma and the incidence of cleft palate. To do so, pregnant mice were randomly divided into two groups. The one group was injected with dexamethasone. On E17.5th day, the incident rates of cleft palate from embryos in two groups were calculated. The (1)H-NMR spectra from the metabolites in plasma in two groups was collected at same time. Then the data were analyzed using metabonomics methods (PCA and SIMCA). The results showed that the data from the two groups displayed distinctive characters, and the incidence of cleft palate were significantly different (P < .005). To conclude, this study demonstrates that the metabonomics approach is a powerful and effective method in detecting the abnormal metabolites from mother in the earlier period of embryos, and supports the idea that a change from dexamethasone induced in maternal metabolites plays an important role in the incidence of cleft palate.
Zhou
Jinglin
J
State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, China.
Xu
Bin
B
Shi
Bing
B
Huang
Jing
J
He
Wei
W
Lu
Shengjun
S
Lu
Junjun
J
Xiao
Liying
L
Li
Wei
W
D000704:Analysis of Variance; D000818:Animals; D015415:Biomarkers / Q000097:blood; D016009:Chi-Square Distribution; D002972:Cleft Palate / Q000097:blood / Q000139:chemically induced* / Q000378:metabolism / Q000473:pathology; D003907:Dexamethasone / Q000633:toxicity*; D005260:Female; D005333:Fetus / Q000473:pathology; D006651:Histocytochemistry; D008297:Male; D055432:Metabolomics / Q000379:methods*; D051379:Mice; D008810:Mice, Inbred C57BL; D011247:Pregnancy; D011296:Prenatal Diagnosis; D025341:Principal Component Analysis
D015415:Biomarkers; D003907:Dexamethasone
NMR Biomed. 2005 May;18(3):143-62
15627238
Am J Obstet Gynecol. 2007 Dec;197(6):585.e1-7; discussion 683-4, e1-7
18060943
Pediatr Res. 2002 Nov;52(5):706-12
12409517
Anal Biochem. 2004 Feb 15;325(2):260-72
14751261
Immunogenetics. 1981;13(5):443-50
7197664
Toxicol Appl Pharmacol. 2005 Apr 15;204(2):135-51
15808519
NMR Biomed. 1998 Jun-Aug;11(4-5):235-44
9719578
AAPS J. 2007 Jul 20;9(3):E284-97
17915830
Arch Oral Biol. 2003 Aug;48(8):581-7
12828987
J Nutr. 1985 Oct;115(10):1291-9
2413189
J Nutr. 2004 Nov;134(11):3106-13
15514283
Anal Biochem. 2001 Jun 1;293(1):16-21
11373073
Bioinformatics. 2002;18 Suppl 2:S241-8
12386008
Chem Res Toxicol. 2000 Jun;13(6):471-8
10858320
Cleft Palate Craniofac J. 1997 Jan;34(1):11-6
9003906
Obstet Gynecol. 2007 Aug;110(2 Pt 1):378-84
17666614
Nat Rev Drug Discov. 2002 Feb;1(2):153-61
12120097
Am J Obstet Gynecol. 2003 Oct;189(4):1155-60
14586370
Epidemiology. 2006 May;17(3):285-91
16570024
Analyst. 2002 Dec;127(12):1549-57
12537357
Anal Biochem. 2001 Aug 15;295(2):194-202
11488622
Teratology. 2002 Nov;66(5):205-8
12397627
Nat Med. 2002 Dec;8(12):1439-44
12447357
Teratology. 1984 Aug;30(1):71-81
6484854
Comput Biomed Res. 1984 Feb;17(1):1-14
6365429
Am J Med Genet C Semin Med Genet. 2004 Feb 15;125C(1):12-21
14755429
Curr Opin Genet Dev. 2005 Jun;15(3):270-8
15917202
J Biol Chem. 2004 Jul 16;279(29):30189-94
15145931
Lancet. 1995 Aug 12;346(8972):393-6
7623568
Cleft Palate J. 1990 Oct;27(4):388-91; discussion 392
2253386
Plast Reconstr Surg. 2000 Feb;105(2):485-91
10697150
Toxicol Appl Pharmacol. 2004 Jul 15;198(2):75-85
15236946
Teratology. 1980 Feb;21(1):39-51
7385055
Biochem Pharmacol. 2002 Jul 1;64(1):67-77
12106607
Semin Orthod. 2008 Jun;14(2):103-114
19492008
Bull World Health Organ. 2004 Mar;82(3):213-8
15112010
Xenobiotica. 1999 Nov;29(11):1181-9
10598751
Plant Mol Biol. 2002 Jan;48(1-2):155-71
11860207
Plast Reconstr Surg. 2005 Apr;115(4):1208-10
15793472
Clin Immunol Immunopathol. 1983 Mar;26(3):335-49
6223748
Science. 1965 Jan 22;147(3656):402-3
14221487
Teratology. 1975 Feb;11(1):47-55
166456
J Pharm Biomed Anal. 2001 Oct;26(3):463-76
11489392