title: Expression and proliferation-promoting role of Diversin in the neuronally committed precursor cells migrating in the adult mouse brain.
abstract: The subventricular zone (SVZ) is the largest neurogenic region in the adult rodent brain. In the adult SVZ, unlike in the embryonic brain, neuronally committed precursor cells (neuroblasts) maintain their proliferative activity while migrating toward the olfactory bulb (OB), suggesting that they are inhibited from exiting the cell cycle. Little is known about the mechanisms underlying the unique ability of adult neuroblasts to proliferate during migration. Here, we studied the expression and function of Diversin, a component of the Wnt signaling pathways. In the neonatal and adult mouse brain, Diversin expression was observed in neuroblasts and mature neurons in the SVZ and hippocampus. Retrovirus-mediated overexpression of Diversin promoted the proliferation of neuroblasts and increased the number of neuroblasts that reached the OB. Conversely, the knockdown of Diversin decreased the proliferation of neuroblasts. Our results indicate that Diversin plays an important role in the proliferation of neuroblasts in the SVZ of the adult brain.
authors.affiliation: Department of Developmental and Regenerative Biology, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan.
authors.forename: Makiko
authors.initials: M
authors.lastname: Ikeda
authors.forename: Yuki
authors.initials: Y
authors.lastname: Hirota
authors.forename: Masanori
authors.initials: M
authors.lastname: Sakaguchi
authors.forename: Osamu
authors.initials: O
authors.lastname: Yamada
authors.forename: Yasuyuki S
authors.initials: YS
authors.lastname: Kida
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Ogura
authors.forename: Takanobu
authors.initials: T
authors.lastname: Otsuka
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Okano
authors.forename: Kazunobu
authors.initials: K
authors.lastname: Sawamoto
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D015153:Blotting, Western; D001921:Brain / Q000166:cytology* / Q000378:metabolism*; D002465:Cell Movement / Q000235:genetics / Q000502:physiology; D049109:Cell Proliferation; D003598:Cytoskeletal Proteins / Q000235:genetics / Q000378:metabolism*; D005260:Female; D005822:Genetic Vectors / Q000235:genetics; D007150:Immunohistochemistry; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008813:Mice, Inbred ICR; D009474:Neurons / Q000166:cytology* / Q000378:metabolism*; D012190:Retroviridae / Q000235:genetics; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism*
chemical_list: C464903:Ankrd6 protein, mouse; D003598:Cytoskeletal Proteins; D047908:Intracellular Signaling Peptides and Proteins