Periostin associates with Notch1 precursor to maintain Notch1 expression under a stress condition in mouse cells.
BACKGROUND
Matricellular proteins, including periostin, modulate cell-matrix interactions and cell functions by acting outside of cells.


RESULTS
In this study, however, we reported that periostin physically associates with the Notch1 precursor at its EGF repeats in the inside of cells. Moreover, by using the periodontal ligament of molar from periostin-deficient adult mice (Pn-/- molar PDL), which is a constitutively mechanically stressed tissue, we found that periostin maintained the site-1 cleaved 120-kDa transmembrane domain of Notch1 (N1) level without regulating Notch1 mRNA expression. N1 maintenance in vitro was also observed under such a stress condition as heat and H(2)O(2) treatment in periostin overexpressed cells. Furthermore, we found that the expression of a downstream effector of Notch signaling, Bcl-xL was decreased in the Pn-/- molar PDL, and in the molar movement, cell death was enhanced in the pressure side of Pn-/- molar PDL.


CONCLUSIONS
These results suggest the possibility that periostin inhibits cell death through up-regulation of Bcl-xL expression by maintaining the Notch1 protein level under the stress condition, which is caused by its physical association with the Notch1 precursor.
Tanabe
Hideyuki
H
Department of Biological Information, Tokyo Institute of Technology, Yokohama, Japan.
Takayama
Issei
I
Nishiyama
Takashi
T
Shimazaki
Masashi
M
Kii
Isao
I
Li
Minqi
M
Amizuka
Norio
N
Katsube
Ken-ichi
K
Kudo
Akira
A
D000818:Animals; D015815:Cell Adhesion Molecules / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D005786:Gene Expression Regulation; D051379:Mice; D008963:Molar / Q000166:cytology* / Q000378:metabolism; D008970:Molecular Weight; D011485:Protein Binding; D011498:Protein Precursors / Q000737:chemistry / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D051881:Receptor, Notch1 / Q000737:chemistry / Q000378:metabolism*; D020449:Repetitive Sequences, Amino Acid; D015398:Signal Transduction; D013314:Stress, Mechanical; D051020:bcl-X Protein / Q000235:genetics / Q000378:metabolism
D015815:Cell Adhesion Molecules; C082838:Postn protein, mouse; D011498:Protein Precursors; D051881:Receptor, Notch1; D051020:bcl-X Protein
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