[Induction of dendritic cells maturation by staphylococcal antigens].
OBJECTIVE
To study the ability to induce dendritic cells maturation obtained from bone marrow of mice by staphylococcal complex of antigens containing bacterial ligands for Toll-like receptors (lipopeptides, lipoproteins, and peptidoglycans).


METHODS
Commercially available TNF-alpha, inductors ofdendritic cells (DCs) maturation, as well C57/BL line mice were used for the study. Immunophenotype of DCs was assessed by flow cytometry and monoclonal antibodies against cellular antigens. Cytological study using phase-contrast microscopy as well as electron microscopy were performed. Levels of cytokines were measured by ELISA.


RESULTS
Obtained DCs had typical morphologic characteristics of mature cells. However the culture was still heterogenous with presence of macrophages that was evident from immunophenotype of obtained cells: CD34(-/+), CD38+, CD40+, CD80+, CD86+, MHC II+, F4/80(-/+). Number of TLR2-expressing cells was also reliably increased in culture of DCs that confirms the presence of corresponding ligands binding with this type of receptor in the preparation. Application of each studied preparation resulted in synthesis of large amount of TNF-alpha, IL-6, IL-1beta, IL-12 by DCs, and IFN-gamma by several types of them (RP and CB24). Although the intensity of induced cytokine synthesis varied for each preparation, it was many times higher compared with immature DCs.


CONCLUSIONS
High synthesis level and wide spectrum of cytokine production demonstrated that under the influence of bacterial ligands DCs acquired characteristics needed for effective antigen presentation and priming of immune response.
Kuz'menko
O M
OM
Akhmatov
E A
EA
Lebedinskaia
E A
EA
D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology*; D001853:Bone Marrow / Q000276:immunology; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology*; D008024:Ligands; D051379:Mice; D008810:Mice, Inbred C57BL; D013210:Staphylococcus / Q000276:immunology*; D051195:Toll-Like Receptor 2 / Q000096:biosynthesis; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D000942:Antigens, Bacterial; D016207:Cytokines; D008024:Ligands; C493486:Tlr2 protein, mouse; D051195:Toll-Like Receptor 2; D014409:Tumor Necrosis Factor-alpha