title: Diazirine-containing RNA photocrosslinking probes for the study of siRNA-protein interactions.
abstract: We here report the synthesis and characterization of small interfering RNAs with aryl trifluoromethyl diazirine moieties in the 3'-overhang regions, which allow sensitive detection of interacting proteins during assembly of the effector ribonucleoprotein complex by irradiation with minimally destructive long-wavelength ultraviolet light.
authors.affiliation: Department of Biomolecular Science, Faculty of Engineering, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
authors.forename: Satoru
authors.initials: S
authors.lastname: Kuboe
authors.forename: Mayuko
authors.initials: M
authors.lastname: Yoda
authors.forename: Aya
authors.initials: A
authors.lastname: Ogata
authors.forename: Yukio
authors.initials: Y
authors.lastname: Kitade
authors.forename: Yukihide
authors.initials: Y
authors.lastname: Tomari
authors.forename: Yoshihito
authors.initials: Y
authors.lastname: Ueno
mesh_terms: D001389:Azirines / Q000737:chemistry*; D001483:Base Sequence; D010777:Photochemistry; D012313:RNA / Q000737:chemistry*; D034741:RNA, Small Interfering; D012689:Sequence Homology, Nucleic Acid; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D001389:Azirines; D034741:RNA, Small Interfering; D012313:RNA