title: Proteomic analysis of differentially expressed proteins in bovine endometrium with endometritis.
abstract: Endometritis is one of the primary reasons for reproductive failure. In order to investigate endometritis-associated marker proteins, proteomic analysis was performed on bovine endometrium with endometritis. In bovine endometritis, desmin, α-actin-2, heat-shock protein (HSP) 27, peroxiredoxin-6, luteinizing hormone receptor isoform 1, collectin-43 precursor, deoxyribonuclease-I (DNase-I), and MHC class I heavy chain (MHC-Ih) were up-regulated. In contrast, transferrin, interleukin-2 precursor, hemoglobin β subunit, and potassium channel tetramerisation domain-containing 11 (KCTD11) were down-regulated in comparison to normal endometrium. The proteomic results were validated by semiquantitative-PCR and immunoblot analysis. The mRNA levels of desmin, transferrin, α-actin-2, HSP27, KCTD11, and MHC-Ih were up-regulated by over 1.5-fold, and showed a pattern similar to their proteomic profiles. Desmin and α-actin-2 protein showed positive correlations between proteomic analysis and immunoblot analysis. These results suggest that desmin and α-actin-2 may play important roles in endometritis-related function, and could be useful markers for the diagnosis of bovine endometritis.
authors.affiliation: Animal Genetic Resources Station, National Institute of Animal Science, RDA, Namwon 590-832, Korea.
authors.forename: Changyong
authors.initials: C
authors.lastname: Choe
authors.forename: Jeong-Won
authors.initials: JW
authors.lastname: Park
authors.forename: Eun-Suk
authors.initials: ES
authors.lastname: Kim
authors.forename: Sung-Gyu
authors.initials: SG
authors.lastname: Lee
authors.forename: Sun-Young
authors.initials: SY
authors.lastname: Park
authors.forename: Jeong-Soon
authors.initials: JS
authors.lastname: Lee
authors.forename: Myung-Je
authors.initials: MJ
authors.lastname: Cho
authors.forename: Kee Ryeon
authors.initials: KR
authors.lastname: Kang
authors.forename: Jaehee
authors.initials: J
authors.lastname: Han
authors.forename: Dawon
authors.initials: D
authors.lastname: Kang
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