title: Differential effects of taurine treatment and taurine deficiency on the outcome of renal ischemia reperfusion injury.
abstract: Taurine possesses membrane stabilization, osmoregulatory and antioxidant properties, aspects of relevance to ischemic injury. We tested the hypothesis that body taurine status is a determinant of renal ischemic injury. Accordingly, renal function and structure were examined in control (C), taurine-treated (TT) and taurine deficient (TD) rats that were subjected to bilateral renal ischemia (60 min) followed by reperfusion (IR); sham operated rats served as controls. Baseline urine osmolality was greater in the TD group than in the control and the TT groups, an effect associated with increased renal aquaporin 2 level. The IR insult reduced urine osmolality (i.e., day-1 post insult); the TD/IR group displayed a more marked recovery in urine osmolality by day-6 post insult than the other two groups. Fluid and sodium excretions were lower in the TD/IR group, suggesting propensity to retention. Histopathological examination revealed the presence of tubular necrotic foci in the C/IR group than sham controls. While renal architecture of the TD/IR group showed features resembling sham controls, the TT/IR group showed dilated tubules, which lacked immunostaining for aquaporin 2, but not 1, suggestive of proximal tubule origin. Finally, assessment of cell proliferation and apoptosis revealed lower proliferation but higher apoptotic foci in the TT/IR group than other IR groups. Collectively, the results indicate that body taurine status is a major determinant of renal IR injury.
authors.affiliation: Department of Oral Biology, Medical College of Georgia School of Dentistry, Augusta, Georgia 30912, USA. Mmozaffa@mail.mcg.edu
authors.forename: Mahmood S
authors.initials: MS
authors.lastname: Mozaffari
authors.forename: Rafik
authors.initials: R
authors.lastname: Abdelsayed
authors.forename: Champa
authors.initials: C
authors.lastname: Patel
authors.forename: Hereward
authors.initials: H
authors.lastname: Wimborne
authors.forename: Jun Yao
authors.initials: JY
authors.lastname: Liu
authors.forename: Stephen W
authors.initials: SW
authors.lastname: Schaffer
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D051399:Aquaporin 2 / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D007668:Kidney / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D007674:Kidney Diseases / Q000188:drug therapy* / Q000503:physiopathology; D007677:Kidney Function Tests; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000503:physiopathology; D013654:Taurine / Q000172:deficiency* / Q000627:therapeutic use*; D016896:Treatment Outcome
chemical_list: D051399:Aquaporin 2; D013654:Taurine
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