Beta-catenin signaling negatively regulates intermediate progenitor population numbers in the developing cortex.
Intermediate progenitor cells constitute a second proliferative cell type in the developing mammalian cerebral cortex. Little is known about the factors that govern the production of intermediate progenitors. Although persistent expression of stabilized beta-catenin was found to delay the maturation of radial glial progenitors into intermediate progenitors, the relationship between beta-catenin signaling and intermediate progenitors remains poorly understood. Using a transgenic reporter mouse for Axin2, a direct target of Wnt/beta-catenin signaling, we observed that beta-catenin signaling is decreased in intermediate progenitor cells relative to radial glial progenitors. Conditional deletion of beta-catenin from mouse cortical neural progenitors increased intermediate progenitor numbers, while conditional expression of stabilized beta-catenin reduced the intermediate progenitor population. Together, these findings provide evidence that beta-catenin signaling in radial progenitors negatively regulates intermediate progenitor cell number during cortical development.
Mutch
Christopher A
CA
Department of Pathology, Northwestern University Feinberg School of Medicine, Chicago, Illinois, United States of America.
Schulte
Jessica D
JD
Olson
Eric
E
Chenn
Anjen
A
D000818:Animals; D002452:Cell Count; D002540:Cerebral Cortex / Q000166:cytology* / Q000254:growth & development* / Q000378:metabolism; D005786:Gene Expression Regulation; D051379:Mice; D057054:Molecular Imaging; D055550:Protein Stability; D015398:Signal Transduction; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism*; D020825:T-Box Domain Proteins / Q000378:metabolism; D051176:beta Catenin / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*
C491198:Eomes protein, mouse; D020825:T-Box Domain Proteins; D051176:beta Catenin
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