title: Development of an immunoassay and a sol-gel-based immunoaffinity cleanup method for coplanar polychlorinated biphenyls from soil and sediment samples.
abstract: Two polychlorinated biphenyls (PCB) enzyme linked immunosorbent assays (ELISAs) were developed using goat PCB purified immunoglobulin (IgG) antibodies (Abs). The IgGs exhibited the highest affinity toward PCB-77 (24 ng mL(-1)) with sensitivities in the range of 6-11 ng mL(-1). The Abs cross-reacted with PCB-126 and the heptachlorodibenzofuran 1,2,3,4,6,7,8-HpCDF but not with PCB-169, PCB-118, Aroclor 1232, 1248, 1260 or the hexachlorodibenzofuran 2,3,4,6,7,8-HxCDF. The IgGs were also used to develop a sol-gel-based immunoaffinity purification (IAP) method for cleanup of PCB-126. Recovery efficiencies depended on the sol-gel formats; a 1:12 format resulted in the highest binding capacity. Net binding capacity ranged from 112 to 257 ng, and 90% of the analyte could be eluted with only 2 mL of ethanol. The method was also very efficient in purifying PCB-126 from spiked soil and sediment samples from contaminated sites; and eliminating matrix interferences to a degree that enabled analysis of the purified samples by ELISA. The approaches developed in the course of the study form a basis for the development of additional IAP methods for other PCBs, and their implementation in high-throughput screening programs for PCB in food, soil, and other environmental and biological samples.
authors.affiliation: Department of Entomology, the Volcani Center, Bet Dagan 50250, Israel. vinnie2@agri.gov.il
authors.forename: Miriam
authors.initials: M
authors.lastname: Altstein
authors.forename: Orna
authors.initials: O
authors.lastname: Ben Aziz
authors.forename: Nir
authors.initials: N
authors.lastname: Skalka
authors.forename: Alisa
authors.initials: A
authors.lastname: Bronshtein
authors.forename: Jane C
authors.initials: JC
authors.lastname: Chuang
authors.forename: Jeanette M
authors.initials: JM
authors.lastname: Van Emon
mesh_terms: D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006041:Goats; D007074:Immunoglobulin G / Q000276:immunology*; D044367:Phase Transition; D011078:Polychlorinated Biphenyls / Q000032:analysis* / Q000276:immunology* / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D012987:Soil / Q000032:analysis*
chemical_list: D007074:Immunoglobulin G; D012987:Soil; D011078:Polychlorinated Biphenyls; C023035:3,4,5,3',4'-pentachlorobiphenyl