title: Multivalent ligands for siglecs.
abstract: Siglecs have emerged as an important family of immunomodulatory glycan-binding proteins that can bind sialoside ligands both on the same cell surface, in cis, and on other cells, in trans. Expression of siglecs varies among a variety of immune cells, and tools to probe siglecs on these cells are crucial to understanding their function. In designing synthetic ligands, competition by cis ligands requires the use of multivalency to achieve sufficient avidity to stably bind siglecs on native cells. This chapter describes the use of multivalent ligands to probe cell surfaces, as well as to investigate ligand binding to recombinant siglecs.
authors.affiliation: Department of Chemical Physiology, The Scripps Research Institute, La Jolla, California, USA.
authors.forename: Mary K
authors.initials: MK
authors.lastname: O'Reilly
authors.forename: James C
authors.initials: JC
authors.lastname: Paulson
mesh_terms: D000818:Animals; D005434:Flow Cytometry; D006801:Humans; D037102:Lectins / Q000235:genetics / Q000378:metabolism*; D008024:Ligands; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000378:metabolism; D018448:Models, Immunological; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D063265:Sialic Acid Binding Immunoglobulin-like Lectins
chemical_list: D037102:Lectins; D008024:Ligands; D008562:Membrane Glycoproteins; D011994:Recombinant Proteins; D063265:Sialic Acid Binding Immunoglobulin-like Lectins
references.citation: Eur J Immunol. 2002 Jul;32(7):1924-32
references.pmid: 12115612
references.citation: Microbiology (Reading). 2007 Sep;153(Pt 9):2817-2822
references.pmid: 17768226
references.citation: Trends Pharmacol Sci. 2009 May;30(5):240-8
references.pmid: 19359050
references.citation: Mol Cell Biol. 2007 Aug;27(16):5699-710
references.pmid: 17562860
references.citation: Biol Chem. 2001 Feb;382(2):291-7
references.pmid: 11308027
references.citation: J Exp Med. 2010 Jan 18;207(1):173-87
references.pmid: 20038598
references.citation: Nat Chem Biol. 2005 Jul;1(2):93-7
references.pmid: 16408005
references.citation: Carbohydr Res. 2002 Oct 8;337(18):1605-13
references.pmid: 12423961
references.citation: J Biol Chem. 1996 Apr 19;271(16):9267-72
references.pmid: 8621587
references.citation: J Biol Chem. 1995 Nov 3;270(44):26184-91
references.pmid: 7592823
references.citation: J Pharmacol Exp Ther. 2009 Aug;330(2):608-12
references.pmid: 19458105
references.citation: Biochem Soc Trans. 2008 Dec;36(Pt 6):1467-71
references.pmid: 19021577
references.citation: Blood. 2010 Jun 10;115(23):4778-86
references.pmid: 20181615
references.citation: Ann N Y Acad Sci. 2008 Nov;1143:61-82
references.pmid: 19076345
references.citation: Infect Immun. 2006 Jul;74(7):4133-41
references.pmid: 16790787
references.citation: Mol Microbiol. 2003 Sep;49(5):1213-25
references.pmid: 12940982
references.citation: FEBS Lett. 2010 Feb 5;584(3):555-61
references.pmid: 19945458
references.citation: Mol Divers. 2006 Nov;10(4):555-66
references.pmid: 16972014
references.citation: Proc Natl Acad Sci U S A. 1998 Jun 23;95(13):7469-74
references.pmid: 9636173
references.citation: J Immunol. 2006 Sep 1;177(5):2994-3003
references.pmid: 16920935
references.citation: J Biol Chem. 2005 Feb 11;280(6):4307-12
references.pmid: 15563466
references.citation: PLoS One. 2008 Apr 16;3(4):e1967
references.pmid: 18414664
references.citation: J Exp Med. 2002 May 6;195(9):1207-13
references.pmid: 11994426
references.citation: J Cell Biol. 1989 Feb;108(2):389-400
references.pmid: 2563728
references.citation: Methods Mol Biol. 2010;600:67-78
references.pmid: 19882121
references.citation: Proc Natl Acad Sci U S A. 2004 Dec 7;101(49):17033-8
references.pmid: 15563589
references.citation: J Biol Chem. 1996 Apr 19;271(16):9273-80
references.pmid: 8621588
references.citation: J Exp Med. 2009 Jan 16;206(1):125-38
references.pmid: 19103880
references.citation: J Exp Med. 2009 Aug 3;206(8):1691-9
references.pmid: 19596804
references.citation: Blood. 2009 Apr 2;113(14):3333-6
references.pmid: 19196661
references.citation: Biochemistry (Mosc). 2006 May;71(5):496-504
references.pmid: 16732727
references.citation: Proc Natl Acad Sci U S A. 2004 Apr 20;101(16):6104-9
references.pmid: 15079087
references.citation: J Am Chem Soc. 2008 Apr 9;130(14):4578-9
references.pmid: 18341338
references.citation: J Biol Chem. 2007 Nov 2;282(44):32200-7
references.pmid: 17728258
references.citation: PLoS One. 2007 Feb 28;2(2):e257
references.pmid: 17330143
references.citation: Org Biomol Chem. 2007 Mar 21;5(6):865-72
references.pmid: 17340000
references.citation: Biochem J. 2001 Apr 15;355(Pt 2):489-97
references.pmid: 11284738
references.citation: Mol Cell Proteomics. 2010 Jun;9(6):1339-51
references.pmid: 20172905
references.citation: ACS Chem Biol. 2008 Sep 19;3(9):567-76
references.pmid: 18729452
references.citation: Nat Rev Immunol. 2007 Apr;7(4):255-66
references.pmid: 17380156
references.citation: Proc Natl Acad Sci U S A. 2009 Feb 24;106(8):2500-5
references.pmid: 19202057
references.citation: J Am Chem Soc. 2008 May 28;130(21):6680-1
references.pmid: 18452295