title: Diagnostic methods for feline coronavirus: a review.
abstract: Feline coronaviruses (FCoVs) are found throughout the world. Infection with FCoV can result in a diverse range of signs from clinically inapparent infections to a highly fatal disease called feline infectious peritonitis (FIP). FIP is one of the most serious viral diseases of cats. While there is neither an effective vaccine, nor a curative treatment for FIP, a diagnostic protocol for FCoV would greatly assist in the management and control of the virus. Clinical findings in FIP are non-specific and not helpful in making a differential diagnosis. Haematological and biochemical abnormalities in FIP cases are also non-specific. The currently available serological tests have low specificity and sensitivity for detection of active infection and cross-react with FCoV strains of low pathogenicity, the feline enteric coronaviruses (FECV). Reverse transcriptase polymerase chain reaction (RT-PCR) has been used to detect FCoV and is rapid and sensitive, but results must be interpreted in the context of clinical findings. At present, a definitive diagnosis of FIP can be established only by histopathological examination of biopsies. This paper describes and compares diagnostic methods for FCoVs and includes a brief account of the virus biology, epidemiology, and pathogenesis.
authors.affiliation: Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, Serdang, Selangor D. E. 43400, Malaysia.
authors.forename: Saeed
authors.initials: S
authors.lastname: Sharif
authors.forename: Siti Suri
authors.initials: SS
authors.lastname: Arshad
authors.forename: Mohd
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authors.lastname: Hair-Bejo
authors.forename: Abdul Rahman
authors.initials: AR
authors.lastname: Omar
authors.forename: Nazariah Allaudin
authors.initials: NA
authors.lastname: Zeenathul
authors.forename: Amer
authors.initials: A
authors.lastname: Alazawy
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