title: Quantitative scintigraphic analysis of pulp revascularization in autotransplanted teeth in dogs.
abstract: OBJECTIVE
  The purpose of this study was to evaluate the pulpal changes associated with autogenous single-rooted immature tooth transplantation in dogs, using either one, or two-stage surgical techniques.
  
  
  METHODS
  Teeth from 3 beagle dogs, 5 months old, were extracted and transplanted to mechanically prepared recipient sockets. Group (A), where the teeth were transplanted using a one-stage method to recipient beds prepared immediately before transplantation. Group (B), where the teeth were transplanted using a two-stage method in which the recipient beds were prepared and left to heal for 7 days before transplantation. Clinical examinations were done every week during 9 weeks. After 9 weeks, the animals were injected with ⁹⁹(m)Technetium hydroxylmethylene diphosphonate (⁹⁹(m)Tc-HMDP) and 3h after injection, a whole body scintigraphic acquisition was performed. After scintigraphic acquisition the animals were euthanized and the teeth extracted and its radioactivity counted in a well counter calibrated to ⁹⁹(m)Tc. With the data obtained, the percentage of activity injected was calculated for each tooth. The data for each group of teeth were evaluated and analyzed using the Mann-Whitney test (p=0.05).
  
  
  RESULTS
  All the transplanted teeth in both groups survived. No statistically significant difference was found in the absorption of the ⁹⁹(m)Tc-HMDP, between the treatment groups (p=0.464) and between them and the control group (Group A vs. control p=0.713 and Group B vs. control p=0.157).
  
  
  CONCLUSIONS
  This study demonstrated that there was no difference between the two surgical techniques in terms of the pulp revascularization in transplanted teeth.
authors.affiliation: Department of Dentistry, University Hospital of Coimbra, Portugal. m.mferreira@netcabo.pt
authors.forename: Manuel Marques
authors.initials: MM
authors.lastname: Ferreira
authors.forename: Maria Filomena
authors.initials: MF
authors.lastname: Botelho
authors.forename: Margarida
authors.initials: M
authors.lastname: Abrantes
authors.forename: Barbara
authors.initials: B
authors.lastname: Oliveiros
authors.forename: Eunice Virgínia
authors.initials: EV
authors.lastname: Carrilho
mesh_terms: D000818:Animals; D003782:Dental Pulp / Q000098:blood supply* / Q000000981:diagnostic imaging; D004285:Dogs; D015902:Gamma Cameras; D007180:Incisor / Q000000981:diagnostic imaging / Q000637:transplantation*; D018919:Neovascularization, Physiologic / Q000502:physiology*; D011877:Radionuclide Imaging; D019275:Radiopharmaceuticals / Q000493:pharmacokinetics; D018709:Statistics, Nonparametric; D013669:Technetium Tc 99m Medronate / Q000493:pharmacokinetics; D014090:Tooth Replantation; D014182:Transplantation, Autologous; D051598:Whole Body Imaging
chemical_list: D019275:Radiopharmaceuticals; D013669:Technetium Tc 99m Medronate