Detergent fraction of heterologous antigen to detect IgA and IgG in strongyloidiasis using saliva and serum paired samples.
Strongyloides stercoralis causes chronic asymptomatic infections in immunocompetent human hosts and systemic invasion in immunocompromised patients, developing into a fatal hyperinfection syndrome. IgA and IgG detection in saliva and serum paired samples were tested using total saline extract from Strongyloides venezuelensis (SE(*)) and its detergent phase (D) extracted with Triton X-114. Saliva and serum paired samples were obtained from: 25 patients with confirmed strongyloidiasis; 25 patients with other parasitoses and 20 from apparently healthy individuals. Sensitivity, specificity, diagnostic efficiency, positive and negative predictive values and likelihood ratio were calculated at the optimum point of reaction. Using D phase sensitivity and specificity to detect IgA in saliva were 76.0% and 88.9% and in serum 80.0% and 86.7%, respectively. To detect IgG, D phase showed sensitivity and specificity of 88.0% and 88.9% in saliva and 88.0% and 84.4% in serum, respectively. D phase proved to be specific and efficient and could be utilized as an alternative antigen for IgA and IgG detection in saliva and serum samples for strongyloidiasis diagnosis.
Ribeiro
Vanessa S
VS
Departamento de Imunologia, Microbiologia e Parasitologia, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Avenida Pará, 1720, CEP 38400-902, Uberlândia, Minas Gerais, Brazil.
Feliciano
Nagilla D
ND
Gonzaga
Henrique T
HT
Costa
Idessânia N
IN
Gonçalves-Pires
Maria R F
MR
Ueta
Marlene T
MT
Costa-Cruz
Julia M
JM
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood / Q000276:immunology; D015478:Antigens, Heterophile / Q000737:chemistry / Q000276:immunology*; D003902:Detergents / Q000737:chemistry; D005243:Feces / Q000469:parasitology; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood / Q000276:immunology*; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology*; D010272:Parasitic Diseases / Q000276:immunology / Q000469:parasitology; D051381:Rats; D012463:Saliva / Q000276:immunology / Q000469:parasitology; D012680:Sensitivity and Specificity; D013321:Strongyloides / Q000276:immunology; D017171:Strongyloides stercoralis / Q000276:immunology; D013322:Strongyloidiasis / Q000097:blood / Q000175:diagnosis / Q000276:immunology*
D000909:Antibodies, Helminth; D015478:Antigens, Heterophile; D003902:Detergents; D007070:Immunoglobulin A; D007074:Immunoglobulin G