Two-component polyethylene glycol surgical sealant influence on intraperitoneal infection in a refined rodent model.
OBJECTIVE
This study determined the influence of a 2-component polyethylene glycol surgical sealant (Coseal) as an adhesion prevention device on sepsis-related mortality and/or systemic bacterial translocation to the spleen.


METHODS
A bacterial inoculum and telemetry probe were implanted in 50 treated and 49 untreated rats. Telemetry probes monitored core-body temperature to determine time of death. Spleens were collected on day 3 for quantitative bacteriology of Escherichia coli and Bacteroides fragilis.


RESULTS
Median survival time and mortality of treated rats (37.0 hours, 54.0%) were noninferior to untreated rats (47.5 hours, 55.1%). Median E coli titers in treated rats (2.24 log colony forming units/spleen) were significantly less than untreated rats (4.32 log colony forming units/spleen). B fragilis titers were not different.


CONCLUSIONS
This study demonstrates intraperitoneal administration of a 2-component polyethylene glycol surgical sealant as an adhesion prevention device does not alter time to death or sepsis-related mortality and/or systemic bacterial translocation to the spleen.
Rodgers
Kathleen E
KE
University of Southern California, Los Angeles, CA, USA.
Burleson
Florence G
FG
Burleson
Gary R
GR
Wolfsegger
Martin J
MJ
Lewis
Kevin M
KM
Redl
Heinz
H
D000818:Animals; D001442:Bacteroides Infections / Q000382:microbiology / Q000517:prevention & control*; D001441:Bacteroides fragilis; D004926:Escherichia coli; D004927:Escherichia coli Infections / Q000382:microbiology / Q000517:prevention & control*; D005260:Female; D053208:Kaplan-Meier Estimate; D010532:Peritoneal Diseases / Q000382:microbiology / Q000517:prevention & control*; D011092:Polyethylene Glycols / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013154:Spleen / Q000382:microbiology*; D013686:Telemetry; D000267:Tissue Adhesions / Q000382:microbiology / Q000517:prevention & control
C513322:CoSeal; D011092:Polyethylene Glycols