[Feasibility of antibiotic selective inhibition method applied for measuring bacterial and fungal activities distribution in activated sludge].
The study evaluated the selective inhibition method for measuring the fungal and bacterial distribution in the activated sludge, using glucose as sole carbon source, and cycloheximide and streptomycin as bactericide and fungicide, respectively, substrate-induced oxygen uptake rate (OUR) as bioactivity index. Three samples were examined, i.e., aerobic sludge in a wastewater treatment plant, soils and anaerobic sludge. As selective microbial biocides were applied, the OUR of aerobic and soils sludge reduced, whereas the OUR of anaerobic sludge was stimulated. When 1 mg/g cycloheximide and 2 mg/g streptomycin were applied, the inhibition index D of aerobic sludge was approximately 1; however, the values of D for soils were greater than 1 at all the dosages of bactericide or fungicide applied in this study. These results suggested that the antibiotics used in this study could selectively inhibit aerobic sludge and partially inhibit the soils. It was found that the selective inhibition method was suitable for aerobic sludge analysis, but encountered problems for the other samples, e.g., failure inhibition effect of antibiotic, partial inhibition and low accuracy. Further studies are therefore needed in the selection of antibiotic and microbial activity parameter.
Li
Zhi-hua
ZH
Key Laboratory of Northwest Water Resources, Environment and Ecolog, Ministry of Education, School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China. lizhihua@gmail.com
Liu
Fang
F
Guo
Qiang
Q
Tan
Zhou-quan
ZQ
Wu
Jie
J
Zhang
Ting
T
Wang
Xiao-chang
XC
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects / Q000254:growth & development*; D001673:Biodegradation, Environmental; D015169:Colony Count, Microbial; D003513:Cycloheximide / Q000494:pharmacology; D005240:Feasibility Studies; D005658:Fungi / Q000187:drug effects / Q000254:growth & development*; D005947:Glucose / Q000378:metabolism; D010101:Oxygen Consumption / Q000187:drug effects; D012722:Sewage / Q000382:microbiology*; D013307:Streptomycin / Q000494:pharmacology; D014865:Waste Disposal, Fluid / Q000379:methods*
D000900:Anti-Bacterial Agents; D012722:Sewage; D003513:Cycloheximide; D005947:Glucose; D013307:Streptomycin