Antimicrobial resistance and investigation of the molecular epidemiology of Listeria monocytogenes in dairy products.
BACKGROUND
Listeria monocytogenes is a ubiquitous microorganism in nature and is responsible for listeriosis, an infectious disease caused by consumption of contaminated food.


METHODS
Molecular characterization was performed on 19 strains of Listeria monocytogenes (serovars 1/2a, 1/2b, 4b and 4c), isolated from dairy products in Rio Grande do Sul, Brazil. The molecular techniques applied were random amplification of polymorphic DNA and restriction enzyme analysis. In addition to the molecular analysis, the antimicrobial resistance profile was determined.


RESULTS
The strains studied showed a low degree of diversity. In relation to the antimicrobial resistance profile of those microorganisms from the samples analyzed, all of them were susceptible to the antimicrobials tested.


CONCLUSIONS
The molecular techniques that were used presented good discriminatory power for the strains studied. Furthermore, all of the samples that were analyzed were susceptible to the antimicrobials tested.
Nes
Fernanda De
FD
Program in Food Science and Technology, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.
Riboldi
Gustavo Pelicioli
GP
Frazzon
Ana Paula Guedes
AP
d'Azevedo
Pedro Alves
PA
Frazzon
Jeverson
J
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001938:Brazil; D004262:DNA Restriction Enzymes; D003611:Dairy Products / Q000382:microbiology*; D005516:Food Microbiology; D008089:Listeria monocytogenes / Q000187:drug effects / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D017720:Molecular Epidemiology; D019105:Random Amplified Polymorphic DNA Technique
D000900:Anti-Bacterial Agents; D004262:DNA Restriction Enzymes