Insecticidal properties of Sclerotinia sclerotiorum agglutinin and its interaction with insect tissues and cells.
This project studied in detail the insecticidal activity of a fungal lectin from the sclerotes of Sclerotinia sclerotiorum, referred to as S. sclerotiorum agglutinin or SSA. Feeding assays with the pea aphid (Acyrthosiphon pisum) on an artificial diet containing different concentrations of SSA demonstrated a high mortality caused by this fungal lectin with a median insect toxicity value (LC50) of 66 (49-88) μg/ml. In an attempt to unravel the mode of action of SSA the binding and interaction of the lectin with insect tissues and cells were investigated. Histofluorescence studies on sections from aphids fed on an artificial liquid diet containing FITC-labeled SSA, indicated the insect midgut with its brush border zone as the primary target for SSA. In addition, exposure of insect midgut CF-203 cells to 25 μg/ml SSA resulted in a total loss of cell viability, the median cell toxicity value (EC50) being 4.0 (2.4-6.7) μg/ml. Interestingly, cell death was accompanied with DNA fragmentation, but the effect was caspase-3 independent. Analyses using fluorescence confocal microscopy demonstrated that FITC-labeled SSA was not internalized in the insect midgut cells, but bound to the cell surface. Prior incubation of the cells with saponin to achieve a higher cell membrane permeation resulted in an increased internalization of SSA in the insect midgut cells, but no increase in cell toxicity. Furthermore, since the toxicity of SSA for CF-203 cells was significantly reduced when SSA was incubated with GalNAc and asialomucin prior to treatment of the cells, the data of this project provide strong evidence that SSA binds with specific carbohydrate moieties on the cell membrane proteins to start a signaling transduction cascade leading to death of the midgut epithelial cells, which in turn results in insect mortality. The potential use of SSA in insect control is discussed.
Hamshou
Mohamad
M
Laboratory of Agrozoology, Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University, Coupure links 653, B-9000 Ghent, Belgium.
Smagghe
Guy
G
Shahidi-Noghabi
Shahnaz
S
De Geyter
Ellen
E
Lannoo
Nausicaä
N
Van Damme
Els J M
EJ
D000116:Acetylgalactosamine / Q000378:metabolism; D000373:Agglutinins / Q000633:toxicity*; D000818:Animals; D001042:Aphids / Q000166:cytology* / Q000187:drug effects*; D001203:Ascomycota / Q000737:chemistry*; D050260:Carbohydrate Metabolism; D016923:Cell Death; D002460:Cell Line; D053938:DNA Fragmentation / Q000187:drug effects; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism; D005656:Fungal Proteins / Q000633:toxicity*; D041981:Gastrointestinal Tract / Q000166:cytology / Q000187:drug effects; D037102:Lectins / Q000633:toxicity; D015398:Signal Transduction
D000373:Agglutinins; D005656:Fungal Proteins; D037102:Lectins; D000116:Acetylgalactosamine