Transport of intensity phase imaging in a volume holographic microscope.
We demonstrate a method for single-shot quantitative phase imaging based on the transport of intensity equation (TIE) in a volume holographic microscope (VHM). The VHM system uses a multiplexed volume hologram to laterally separate images from different focal planes. This axial intensity information is then used to solve the TIE and recover object phase quantitatively. Further, we show improved phase recovery by using five multiplexed gratings in one hologram.
Waller
Laura
L
Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA. lwaller@alum.mit.edu
Luo
Yuan
Y
Yang
Se Young
SY
Barbastathis
George
G
D006696:Holography / Q000379:methods*; D007091:Image Processing, Computer-Assisted; D008853:Microscopy / Q000295:instrumentation / Q000379:methods*; D019697:Onions / Q000033:anatomy & histology; D019441:Plant Epidermis