title: Life extension by diet restriction and N-acetyl-L-cysteine in genetically heterogeneous mice.
abstract: We used a heterogeneous stock of mice-UM-HET3, the first generation offspring of CByB6F1/J and C3D2F1/J parents-to test effects of six antiaging treatments on life span. In the first report of diet restriction in a structured, segregating heterogeneous population, we observed essentially the same increases in mean and maximum life span as found in CByB6F1/J hybrid positive controls. We also report results of treatment with N-acetyl-L-cysteine started at 7 months, and aspirin, nitroflurbiprofen, 4-hydroxy phenyl N-tert-butyl nitrone, and nordihydroguaiaretic acid, all started at 16-18 months. Only male UM-HET3 mice receiving N-acetyl-L-cysteine had significantly increased life span, and this may have been due to treatment-related inadvertent diet restriction. The other agents had no significant effects on life span. The use of UM-HET3 mice helps assure that these results are not the result of unresponsiveness of a single genotype but that they more broadly represent laboratory mice.
authors.affiliation: The Jackson Laboratory, 600 Main Street, Bar Harbor, ME 04609, USA.
authors.forename: Kevin
authors.initials: K
authors.lastname: Flurkey
authors.forename: Clinton M
authors.initials: CM
authors.lastname: Astle
authors.forename: David E
authors.initials: DE
authors.lastname: Harrison
mesh_terms: D000111:Acetylcysteine / Q000494:pharmacology*; D000818:Animals; D001241:Aspirin / Q000494:pharmacology; D003433:Crosses, Genetic; D004032:Diet; D005260:Female; D005480:Flurbiprofen / Q000031:analogs & derivatives / Q000494:pharmacology; D007097:Imines / Q000494:pharmacology; D008017:Life Expectancy; D008136:Longevity / Q000187:drug effects; D008297:Male; D009637:Masoprocol / Q000494:pharmacology; D051379:Mice; D008815:Mice, Inbred Strains / Q000235:genetics*; D010636:Phenols / Q000494:pharmacology; D012737:Sex Factors
chemical_list: C554675:4-hydroxyphenyl tert-butylnitrone; D007097:Imines; D010636:Phenols; C095631:nitroflurbiprofen; D005480:Flurbiprofen; D009637:Masoprocol; D001241:Aspirin; D000111:Acetylcysteine
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