title: [Improvement of method of Coxiella burnetii detection in biological material based on groEL gene amplification].
abstract: OBJECTIVE
  Improvement of PCR for detection of Coxiella burnetii in field material by development of set of primers for amplification of groEL gene fragment.
  
  
  METHODS
  C. burnetii strains, samples from organs of wild rodents and laboratory animals, blood of healthy donors and laboratory animals. Methods of DNA isolation, PCR, and electrophoresis in agarose gel were used.
  
  
  RESULTS
  Nucleotide sequences of primers amplifying groEL gene fragment, which could increase specificity of PCR during work with field material were proposed.
  
  
  CONCLUSIONS
  Obtained data open perspective for using this method for detection of C. burnetii in environment.
authors.forename: O A
authors.initials: OA
authors.lastname: Freĭlikhman
authors.forename: Iu A
authors.initials: IuA
authors.lastname: Panferova
authors.forename: N K
authors.initials: NK
authors.lastname: Tokarevich
mesh_terms: D000818:Animals; D018834:Chaperonin 60 / Q000235:genetics*; D016997:Coxiella burnetii / Q000235:genetics / Q000302:isolation & purification*; D017931:DNA Primers; D004197:Disease Reservoirs; D005798:Genes, Bacterial / Q000235:genetics; D006801:Humans; D007668:Kidney / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D011778:Q Fever / Q000175:diagnosis* / Q000382:microbiology; D012377:Rodentia / Q000382:microbiology; D012426:Russia; D012680:Sensitivity and Specificity; D013154:Spleen / Q000382:microbiology
chemical_list: D018834:Chaperonin 60; D017931:DNA Primers