Chemo-resistant protein expression pattern of glioblastoma cells (A172) to perillyl alcohol.
Glioblastoma multiform (GBM) is by far the most malignant glioma. We have introduced a new treatment for GBMs that comprises the inhalation of a naturally occurring terpene with chemotherapeutic properties known as perillyl alcohol (POH). Clinical trial results on recurrent GBM patients showed that POH extends the average life by more than eight months, temporarily slows tumor growth, and in some cases even decreases tumor size. After approximately seven months, the tumor continues to grow and leads to a dismal prognosis. To investigate how these tumors become resistant to POH, we generated an A172 human glioblastoma cell culture tolerant to 0.06 mM of POH (A172r). We used Multidimensional Protein Identification Technology (MudPIT) to compare the protein expression profile of A172r cells to the established glioblastoma A172 cell line. Our results include a list of identified proteins unique to either the resistant or the nonresistant cell line. These proteins are related to cellular growth, negative apoptosis regulation, Ras pathway, and other key cellular functions that could be connected to the underlying mechanisms of resistance.
de Saldanha da Gama Fischer
Juliana
J
Center for Technological Development in Health - Oswaldo Cruz Foundation, Ministry of Health, Brazil.
Costa Carvalho
Paulo
P
da Fonseca
Clovis Orlando
CO
Liao
Lujian
L
Degrave
Wim M
WM
da Gloria da Costa Carvalho
Maria
M
Yates
John R
JR
Domont
Gilberto B
GB
D000970:Antineoplastic Agents / Q000494:pharmacology*; D015153:Blotting, Western; D001921:Brain / Q000473:pathology; D045744:Cell Line, Tumor; D002986:Clinical Trials as Topic; D019008:Drug Resistance, Neoplasm / Q000187:drug effects*; D015180:Electrophoresis, Gel, Two-Dimensional; D005909:Glioblastoma / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D008279:Magnetic Resonance Imaging; D039821:Monoterpenes / Q000494:pharmacology*; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D020543:Proteome / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D015203:Reproducibility of Results; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D000970:Antineoplastic Agents; D039821:Monoterpenes; D010446:Peptide Fragments; D020543:Proteome; C032208:perillyl alcohol
Proteomics. 2003 Aug;3(8):1620-32
12923786
Clin Cancer Res. 2004 Nov 15;10(22):7583-91
15569989
J Am Soc Mass Spectrom. 1994 Nov;5(11):976-89
24226387
BMC Bioinformatics. 2008 Jul 21;9:316
18644148
J Natl Cancer Inst. 2000 Oct 4;92(19):1564-72
11018092
Nat Biotechnol. 2001 Mar;19(3):242-7
11231557
J Proteome Res. 2005 Nov-Dec;4(6):2148-53
16335961
Proc Natl Acad Sci U S A. 2000 Jul 5;97(14):7871-6
10884417
J Proteome Res. 2002 Jan-Feb;1(1):21-6
12643522
Prostate. 2003 Sep 15;57(1):14-23
12886519
Oncology. 2006;70(1):13-8
16439861
Oncogene. 1999 Jun 17;18(24):3648-51
10380887
Genet Mol Res. 2008 Apr 15;7(2):342-56
18551400
Stem Cells. 2010 Jan;28(1):17-28
19921751
Trends Cell Biol. 2001 Jan;11(1):6-10
11146277
Biochim Biophys Acta. 2004 Jan 20;1688(1):43-60
14732480
Glia. 2000 Nov;32(2):146-54
11008214
Cancer Lett. 2007 Nov 18;257(2):216-26
17888568
Biochim Biophys Acta. 2003 Aug 21;1650(1-2):73-91
12922171
Cancer Lett. 2006 May 18;236(2):222-8
16029925
Int J Gastrointest Cancer. 2002;32(2-3):125-8
12794248
BMC Cancer. 2006 Apr 12;6:92
16608533
J Biol Chem. 2000 Jun 30;275(26):19521-8
10751413
BMC Cancer. 2009 Jun 26;9:206
19558675
Cancer Res. 1995 Mar 1;55(5):979-83
7867007
Oncol Rep. 2005 May;13(5):943-7
15809762
Gynecol Oncol. 2002 Jun;85(3):464-8
12051875
Cancer Res. 2008 Jan 1;68(1):132-42
18172305
Cancer Lett. 2002 Jul 26;181(2):187-94
12175534
Nutr Cancer. 1998;32(1):1-7
9824849
J Exp Ther Oncol. 2008;7(4):285-90
19227008
J Proteomics. 2010 Mar 10;73(5):1018-27
20083244
Breast Cancer Res Treat. 2004 Apr;84(3):251-60
15026623
J Pharmacol Exp Ther. 2004 Nov;311(2):568-75
15210838
Biochem Pharmacol. 1999 Apr 1;57(7):801-9
10075086
Anal Chem. 2004 Jul 15;76(14):4193-201
15253663
J Exp Med. 1993 Jan 1;177(1):231-6
8418204
Biochem Pharmacol. 1994 Apr 20;47(8):1405-15
8185648
Rapid Commun Mass Spectrom. 2004;18(18):2162-8
15317041
J Neurosurg. 2000 Mar;92(3):428-34
10701529
Surg Neurol. 2008 Sep;70(3):259-66; discussion 266-7
18295834
Proteome Sci. 2009 Feb 24;7:6
19239707
Clin Cancer Res. 2003 Oct 1;9(12):4494-504
14555523
Clin Exp Metastasis. 1988 Jul-Aug;6(4):257-69
3359710
Mol Cancer Ther. 2006 Sep;5(9):2182-92
16985051
Int J Mol Med. 2002 Dec;10(6):785-8
12430008
Proteomics. 2004 Jul;4(7):1985-8
15221759
J Proteome Res. 2003 Jan-Feb;2(1):43-50
12643542
Genomics. 1992 Jan;12(1):74-9
1346391