title: Expressions of matrix metalloproteinase-7 and matrix metalloproteinase-14 associated with the activation of extracellular signal-regulated kinase1/2 in human brain gliomas of different pathological grades.
abstract: The purpose of this study is to determine whether the expressions of MMP-7 and MMP-14 are associated with the ERK 1/2 signaling pathway in human brain gliomas of different pathological grades. Immunohistochemistry and western blot methods were used to determine the expressions of MMP-7, MMP-14 and the phosphorylation status of ERK1/2 in 73 cases of human brain glioma specimens and two cases of normal brain tissues. Results indicated that the protein expression levels of MMP-7, MMP-14 and ERK1/2 phosphorylation level were all elevated with the increasing pathological grades in brain glioma tissues, and correlation assay indicated that the level of ERK1/2 phosphorylation was positively correlated with protein expression levels of MMP-7 and MMP-14 in gliomas of different pathological grades respectively. Moreover, the impact of ERK1/2 inhibitor U0126 on the expressions of MMP-7 and MMP-14 was examined in human U87 glioma cells by western blot analysis. The expressions of MMP-7 and MMP-14 were significantly decreased in human U87 glioma cells after treatment with ERK1/2 inhibitor U0126. The above results suggest that the expressions of MMP-7 and MMP-14 may be associated with activation of ERK1/2 signaling pathway in human brain gliomas of different pathological grades.
authors.affiliation: Department of Neurobiology, College of Basic Medicine, China Medical University, 110001 Shenyang, Liaoning Province, People's Republic of China.
authors.forename: Hui
authors.initials: H
authors.lastname: Xie
authors.forename: Yi-xue
authors.initials: YX
authors.lastname: Xue
authors.forename: Li-bo
authors.initials: LB
authors.lastname: Liu
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.forename: Yun-hui
authors.initials: YH
authors.lastname: Liu
authors.forename: Hao-qiang
authors.initials: HQ
authors.lastname: Ying
mesh_terms: D000328:Adult; D000368:Aged; D001932:Brain Neoplasms / Q000201:enzymology* / Q000473:pathology; D045744:Cell Line, Tumor; D004789:Enzyme Activation; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D005910:Glioma / Q000201:enzymology* / Q000473:pathology; D006801:Humans; D020935:MAP Kinase Signaling System; D008297:Male; D053511:Matrix Metalloproteinase 14 / Q000378:metabolism*; D020783:Matrix Metalloproteinase 7 / Q000378:metabolism*; D008875:Middle Aged; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism; D060787:Neoplasm Grading; D055815:Young Adult
chemical_list: C535150:MAPK1 protein, human; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; C060932:MMP7 protein, human; D020783:Matrix Metalloproteinase 7; D053511:Matrix Metalloproteinase 14
references.citation: Nihon Rinsho. 2003 Sep;61 Suppl 7:209-14
references.pmid: 14574883
references.citation: Biochem Biophys Res Commun. 2008 Dec 5;377(1):195-9
references.pmid: 18834856
references.citation: J Immunol. 2001 Nov 1;167(9):5150-9
references.pmid: 11673527
references.citation: Neuropathology. 2008 Oct;28(5):507-15
references.pmid: 18410277
references.citation: J Biol Chem. 2000 Apr 14;275(15):11368-78
references.pmid: 10753951
references.citation: Nat Rev Cancer. 2003 Jul;3(7):489-501
references.pmid: 12835669
references.citation: Am J Pathol. 2005 Mar;166(3):877-90
references.pmid: 15743799
references.citation: Cancer Invest. 2007 Oct;25(7):574-83
references.pmid: 18027151
references.citation: Oncol Rep. 2008 Apr;19(4):1007-13
references.pmid: 18357389
references.citation: J Biol Chem. 2004 May 28;279(22):23151-7
references.pmid: 15047712
references.citation: Am J Pathol. 1999 Feb;154(2):417-28
references.pmid: 10027400
references.citation: Semin Cancer Biol. 2000 Dec;10(6):415-33
references.pmid: 11170864
references.citation: Exp Biol Med (Maywood). 2006 Jan;231(1):20-7
references.pmid: 16380641
references.citation: Neuropathology. 2005 Mar;25(1):48-53
references.pmid: 15822818
references.citation: J Gastroenterol. 2007 Feb;42(2):120-8
references.pmid: 17351800
references.citation: Oncol Rep. 2006 Oct;16(4):823-30
references.pmid: 16969501
references.citation: Mol Cell Biol. 1996 Mar;16(3):1027-34
references.pmid: 8622647
references.citation: Int J Mol Med. 2005 Oct;16(4):541-6
references.pmid: 16142384
references.citation: Semin Cancer Biol. 1996 Jun;7(3):147-54
references.pmid: 8773300
references.citation: J Pathol. 1999 Nov;189(3):300-8
references.pmid: 10547590
references.citation: Cancer Metastasis Rev. 2003 Dec;22(4):395-403
references.pmid: 12884914
references.citation: Int J Cancer. 2000 Jan 1;85(1):14-20
references.pmid: 10585576
references.citation: Mol Carcinog. 2007 Jun;46(6):446-52
references.pmid: 17219436
references.citation: Carcinogenesis. 2008 Aug;29(8):1519-27
references.pmid: 18487224
references.citation: Lung Cancer. 2007 Dec;58(3):384-91
references.pmid: 17728005
references.citation: J Oral Pathol Med. 2007 Aug;36(7):415-24
references.pmid: 17617835
references.citation: Gynecol Oncol. 2009 Feb;112(2):319-24
references.pmid: 18976802
references.citation: J Neurooncol. 2001 Jun;53(2):213-35
references.pmid: 11716072
references.citation: Oral Oncol. 2008 Jun;44(6):587-94
references.pmid: 17936676