title: Crystallization and preliminary X-ray diffraction analysis of rat autotaxin.
abstract: Rat autotaxin has been cloned, expressed, purified to homogeneity and crystallized via hanging-drop vapour diffusion using PEG 3350 as precipitant and ammonium iodide and sodium thiocyanate as salts. The crystals diffracted to a maximum resolution of 2.05 A and belonged to space group P1, with unit-cell parameters a=53.8, b=63.3, c=70.5 A, alpha=98.8, beta=106.2, gamma=99.8 degrees. Preliminary X-ray diffraction analysis indicated the presence of one molecule per asymmetric unit, with a solvent content of 47%.
authors.affiliation: Pfizer Global Research and Development, St Louis Laboratories, 700 Chesterfield Parkway West, Chesterfield, MO 63017, USA.
authors.forename: Jacqueline E
authors.initials: JE
authors.lastname: Day
authors.forename: Troii
authors.initials: T
authors.lastname: Hall
authors.forename: Lyle E
authors.initials: LE
authors.lastname: Pegg
authors.forename: Timothy E
authors.initials: TE
authors.lastname: Benson
authors.forename: Jens
authors.initials: J
authors.lastname: Hausmann
authors.forename: Satwik
authors.initials: S
authors.lastname: Kamtekar
mesh_terms: D000818:Animals; D003460:Crystallization; D018360:Crystallography, X-Ray; D010727:Phosphoric Diester Hydrolases / Q000737:chemistry*; D011755:Pyrophosphatases / Q000737:chemistry*; D051381:Rats; D000099189:Lysophospholipase D
chemical_list: D010727:Phosphoric Diester Hydrolases; D011755:Pyrophosphatases; D000099189:Lysophospholipase D
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