[Improvement of method of Coxiella burnetii detection in biological material based on groEL gene amplification].
OBJECTIVE
Improvement of PCR for detection of Coxiella burnetii in field material by development of set of primers for amplification of groEL gene fragment.


METHODS
C. burnetii strains, samples from organs of wild rodents and laboratory animals, blood of healthy donors and laboratory animals. Methods of DNA isolation, PCR, and electrophoresis in agarose gel were used.


RESULTS
Nucleotide sequences of primers amplifying groEL gene fragment, which could increase specificity of PCR during work with field material were proposed.


CONCLUSIONS
Obtained data open perspective for using this method for detection of C. burnetii in environment.
Freĭlikhman
O A
OA
Panferova
Iu A
IuA
Tokarevich
N K
NK
D000818:Animals; D018834:Chaperonin 60 / Q000235:genetics*; D016997:Coxiella burnetii / Q000235:genetics / Q000302:isolation & purification*; D017931:DNA Primers; D004197:Disease Reservoirs; D005798:Genes, Bacterial / Q000235:genetics; D006801:Humans; D007668:Kidney / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D011778:Q Fever / Q000175:diagnosis* / Q000382:microbiology; D012377:Rodentia / Q000382:microbiology; D012426:Russia; D012680:Sensitivity and Specificity; D013154:Spleen / Q000382:microbiology
D018834:Chaperonin 60; D017931:DNA Primers