Stereoselective analysis of ritodrine diastereomers in human serum using HPLC.
A sensitive and selective method has been developed for the determination of ritodrine diastereomers in human serum using high-performance liquid chromatography with a chiral stationary phase column and a fluorescence detector. No interfering peaks from endogenous substances were observed. The method showed good reproducibility and accuracy, and the standard curve was linear up to 100 ng/mL with a correlation coefficient of 0.999. Limit of detection (signal-to-noise = 3) and quantitation (signal-to-noise = 10) were found to be 2 and 5 ng/mL, respectively. This method is suitable for chiral pharmacological and pharmacokinetic studies as well as the therapeutic drug monitoring of ritodrine diastereomers for which no information currently exists.
Konda
Ainari
A
Department of Pharmacy, Tenshi Hospital, Sapporo, Japan.
Soma
Mayuko
M
Ito
Taku
T
Yakeuchi
Yuriko
Y
Toda
Takaki
T
Hayakawa
Toru
T
Inotsume
Nobuo
N
D000328:Adult; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D016014:Linear Models; D011247:Pregnancy; D015203:Reproducibility of Results; D012312:Ritodrine / Q000008:administration & dosage / Q000097:blood* / Q000737:chemistry; D012680:Sensitivity and Specificity; D013237:Stereoisomerism; D014427:Twins
D012312:Ritodrine