EMR-3: a potential mediator of invasive phenotypic variation in glioblastoma and novel therapeutic target.
Epidermal growth factor module-containing mucin-like hormone receptor-3 (EMR-3) is a G-protein coupled receptor with unknown ligand and cellular function. Upregulation of EMR-3 in glioblastoma (GBM) multiforme is associated with poor survival. We investigated the expression patterns and functional significance of EMR-3 in GBM using immunohistochemistry, western blot, reverse transcription PCR, and small interfering RNA knockdown in proliferation and invasion assays. EMR-3 is variably expressed in primary human GBM tissues and cell lines. Knocking down EMR-3 has no impact on cellular proliferation, but decreases cellular invasion by greater than 3-fold. EMR-3 is a potential mediator of cellular invasion in GBM. Given the poor survival associated with high levels of EMR-3 expression in glioma patients, our results provide impetus to explore EMR-3 as a potential therapeutic target.
Kane
Ari J
AJ
Department of Neurological Surgery, Division of Neuropathology, University of California at San Francisco, San Francisco, California 94143, USA.
Sughrue
Michael E
ME
Rutkowski
Martin J
MJ
Phillips
Joanna J
JJ
Parsa
Andrew T
AT
D014408:Biomarkers, Tumor / Q000235:genetics; D001932:Brain Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D003937:Diagnosis, Differential; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D005909:Glioblastoma / Q000235:genetics / Q000378:metabolism* / Q000503:physiopathology; D006801:Humans; D009361:Neoplasm Invasiveness / Q000235:genetics / Q000473:pathology*; D010641:Phenotype; D034622:RNA Interference / Q000502:physiology; D043562:Receptors, G-Protein-Coupled / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D018000:Receptors, Peptide / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D015996:Survival Rate / Q000639:trends
C431147:ADGRE3 protein, human; D014408:Biomarkers, Tumor; D043562:Receptors, G-Protein-Coupled; D018000:Receptors, Peptide
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