A novel reactive ester derivative of biotin with reduced membrane permeability for in vivo biotinylation experiments.
The in vivo perfusion of rodent models of disease with biotin derivatives and the subsequent comparative proteomic analysis of healthy and diseased tissues represent a promising methodology for the identification of vascular accessible biomarkers. A novel, triply charged biotinylation reagent, NHS-β-Ala-(L-Asp)(3)-biotin, was synthesized and validated in terms of its applicability for in vivo protein biotinylation. Compared to sulfo-NHS-LC-biotin, NHS-β-Ala-(L-Asp)(3)-biotin exhibited a reduced membrane permeability and a preferential labeling of proteins localized in compartments readily accessible in vivo from the vasculature.
Strassberger
Verena
V
Department of Chemistry and Applied Biosciences, ETH Zurich, Zurich, Switzerland.
Trüssel
Sabrina
S
Fugmann
Tim
T
Neri
Dario
D
Roesli
Christoph
C
D000818:Animals; D001710:Biotin / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry; D019921:Biotinylation / Q000379:methods*; D002463:Cell Membrane Permeability; D007668:Kidney / Q000737:chemistry; D008099:Liver / Q000737:chemistry; D051379:Mice; D011506:Proteins / Q000032:analysis*; D013388:Succinimides / Q000737:chemistry
D011506:Proteins; D013388:Succinimides; C061443:sulfosuccinimidyl 6-(biotinamido)hexanoate; D001710:Biotin