title: Strain- and age-associated variation in viral persistence and antibody response to mouse parvovirus 1 in experimentally infected mice.
abstract: The effect of mouse strain and age at infection on viral replication and concurrent antibody response to mouse parvovirus 1 (isolate MPV1f) was evaluated for 305 d after inoculation in 4 strains of mice. The results confirmed previous reports that mouse strain and age at infection are significant factors in viral persistence and antibody development and detection. Randombred Arc:Arc(s) mice originally bred from CD1 stock inoculated as juveniles (4 wk) or adults (8 wk) developed persistent viral infection for 152 d after inoculation and an antibody response that persisted for 295 d. Mice of C57BL/6J background inoculated as juveniles had detectable viral DNA in large intestinal content and tissues for 24 d after inoculation and an antibody response that persisted for 288 d. However, viral DNA was not detected in tissues of C57BL/6J mice inoculated as adults, although an antibody was detected for 111 d after inoculation; these results suggest probable viral replication in adult C57BL/6J mice but at levels below the limits of detection. BALB/cArc mice inoculated as juveniles or adults had detectable virus DNA in tissues for 108 to 242 d after inoculation, but no antibody was detected. Similarly, BALB/c-Foxn1(nu)/Arc mice had detectable levels of viral DNA in tissues for 98 to 131 d but no measurable antibody. The difficulty of detecting antibody in mice with a BALB/c background indicates they are unsuitable for routine surveillance of MPV1f infection.
authors.affiliation: School of Veterinary and Biomedical Sciences, Murdoch University, Australia.
authors.forename: Emilija
authors.initials: E
authors.lastname: Filipovska-Naumovska
authors.forename: Martin J
authors.initials: MJ
authors.lastname: Thompson
authors.forename: Deborah
authors.initials: D
authors.lastname: Hopwood
authors.forename: David A
authors.initials: DA
authors.lastname: Pass
authors.forename: Graham E
authors.initials: GE
authors.lastname: Wilcox
mesh_terms: D000367:Age Factors; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis*; D004279:DNA, Viral / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains / Q000276:immunology* / Q000821:virology; D010322:Parvoviridae Infections / Q000276:immunology / Q000662:veterinary*; D017992:Parvovirus / Q000276:immunology*; D012307:Risk Factors; D012376:Rodent Diseases / Q000276:immunology / Q000821:virology*
chemical_list: D000914:Antibodies, Viral; D004279:DNA, Viral
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