title: Phylogeny and systematics of the genus Calonectria.
abstract: Species of Calonectria are important plant pathogens, several of which have a worldwide distribution. Contemporary taxonomic studies on these fungi have chiefly relied on DNA sequence comparisons of the beta-tubulin gene region. Despite many new species being described, there has been no phylogenetic synthesis for the group since the last monographic study almost a decade ago. In the present study, the identity of a large collection of Calonectria isolates from various geographic regions was determined using morphological and DNA sequence comparisons. This resulted in the discovery of seven new species; Ca. densa, Ca. eucalypti, Ca. humicola, Ca. orientalis, Ca. pini, Ca. pseudoscoparia and Ca. sulawesiensis, bringing the total number of currently accepted Calonectria species to 68. A multigene phylogeny was subsequently constructed for all available Calonectria spp., employing seven gene regions, namely actin, beta-tubulin, calmodulin, histone H3, the internal transcribed spacer regions 1 and 2 and the 5.8S gene of the ribosomal RNA, 28S large subunit RNA gene and translation elongation 1-alpha. Based on these data 13 phylogenetic groups could be distinguished within the genus Calonectria that correlated with morphological features. Dichotomous and synoptic keys to all Calonectria spp. currently recognised are also provided.
authors.affiliation: Department of Microbiology and Plant Pathology, Tree Protection Co-operative Programme, Forestry and Agricultural Biotechnology Institute, University of Pretoria, Pretoria 0002, South Africa.
authors.forename: L
authors.initials: L
authors.lastname: Lombard
authors.forename: P W
authors.initials: PW
authors.lastname: Crous
authors.forename: B D
authors.initials: BD
authors.lastname: Wingfield
authors.forename: M J
authors.initials: MJ
authors.lastname: Wingfield
references.citation: Mycol Res. 2007 May;111(Pt 5):509-47
references.pmid: 17572334
references.citation: Syst Biol. 2009 Apr;58(2):224-39
references.pmid: 20525580
references.citation: Curr Microbiol. 1996 Oct;33(4):249-55
references.pmid: 8824171
references.citation: Mol Biol Evol. 1997 Jul;14(7):733-40
references.pmid: 9214746
references.citation: Persoonia. 2009 Dec;23:41-7
references.pmid: 20198160
references.citation: Evolution. 2003 Dec;57(12):2703-20
references.pmid: 14761051
references.citation: Mycoses. 1998 May-Jun;41(5-6):183-9
references.pmid: 9715630
references.citation: Bioinformatics. 1998;14(9):817-8
references.pmid: 9918953
references.citation: Bioinformatics. 2003 Aug 12;19(12):1572-4
references.pmid: 12912839
references.citation: Plant Dis. 2001 Sep;85(9):941-946
references.pmid: 30823106
references.citation: Stud Mycol. 2010;66:15-30
references.pmid: 20806004
references.citation: Mol Phylogenet Evol. 1997 Feb;7(1):103-16
references.pmid: 9007025
references.citation: Syst Appl Microbiol. 2001 Jul;24(2):206-17
references.pmid: 11518323
references.citation: Bioinformatics. 2009 Jun 1;25(11):1451-2
references.pmid: 19346325
references.citation: Persoonia. 2010 Jun;24:1-11
references.pmid: 20664755
references.citation: Plant Dis. 2007 Aug;91(8):1052
references.pmid: 30780444
references.citation: Stud Mycol. 2010;66:1-13
references.pmid: 20806003
references.citation: Proc Natl Acad Sci U S A. 1998 Mar 3;95(5):2044-9
references.pmid: 9482835
references.citation: J Bacteriol. 1990 Aug;172(8):4238-46
references.pmid: 2376561
references.citation: Mycol Res. 2007 Oct;111(Pt 10):1145-68
references.pmid: 17981450
references.citation: FEMS Microbiol Lett. 2003 Dec 5;229(1):127-32
references.pmid: 14659552
references.citation: Stud Mycol. 2006;55:213-26
references.pmid: 18490981
references.citation: Fungal Genet Biol. 2000 Oct;31(1):21-32
references.pmid: 11118132
references.citation: Mycologia. 2002 Nov-Dec;94(6):980-97
references.pmid: 21156571
references.citation: Stud Mycol. 2006;55:147-61
references.pmid: 18490976
references.citation: Stud Mycol. 2006;55:227-34
references.pmid: 18490982
references.citation: J Mol Biol. 1990 Oct 5;215(3):403-10
references.pmid: 2231712
references.citation: Nucleic Acids Res. 2005 Jan 20;33(2):511-8
references.pmid: 15661851