Identification and development of a promising novel mumps vaccine candidate strain.
Mumps epidemics are usually caused by airborne transmission of mumps virus (MuV) and have high morbidity in non-immunized children. Epidemiological studies in many regions of China show that the genotype F viral strain is the most prevalent. However, the genotype A strain is currently used to prepare vaccines. Regional epidemiological MuV data suggest a significant application for the development of live attenuated mumps vaccines targeting specific genotypes. This article reports the isolation and culture of a genotype F MuV candidate strain that could be used to prepare a live attenuated mumps vaccine. This strain is shown to have good immunological efficacy and stability in neurovirulence evaluations. This work should facilitate the implementation of mumps vaccination in mainland China by targeting the most prevalent MuV genotype, genotype F.
Liang
Yan
Y
Institute of Medical Biology, Chinese Academy of Medicine Science, Peking Union Medical College, 379# Jiaoling Road, Kunming 650118, PR China.
Ma
Shaohui
S
Liu
Longding
L
Zhao
Hongling
H
Wang
Lichun
L
Jiang
Li
L
Xie
Zhongping
Z
Dong
Chenghong
C
Li
Qihan
Q
D000220:Adaptation, Biological; D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood; D002648:Child; D002681:China / Q000453:epidemiology; D002522:Chlorocebus aethiops; D004195:Disease Models, Animal; D005838:Genotype; D006801:Humans; D008253:Macaca mulatta; D008297:Male; D008969:Molecular Sequence Data; D009107:Mumps / Q000453:epidemiology* / Q000473:pathology / Q000517:prevention & control; D009108:Mumps Vaccine / Q000235:genetics / Q000276:immunology*; D009109:Mumps virus / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification / Q000472:pathogenicity; D009500:Neutralization Tests; D010802:Phylogeny; D017354:Point Mutation; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA; D012692:Serial Passage; D014613:Vaccines, Attenuated / Q000235:genetics / Q000276:immunology; D014709:Vero Cells
D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D009108:Mumps Vaccine; D012367:RNA, Viral; D014613:Vaccines, Attenuated