title: The exon junction complex differentially marks spliced junctions.
abstract: The exon junction complex (EJC), which is deposited onto mRNAs as a consequence of splicing, is involved in multiple post-transcriptional events in metazoa. Here, using Drosophila melanogaster cells, we show that only some introns trigger EJC-dependent nonsense-mediated mRNA decay and that EJC association with particular spliced junctions depends on RNA cis-acting sequences. This study provides the first evidence to our knowledge that EJC deposition is not constitutive but instead is a regulated process.
authors.affiliation: Institut de Biologie de l'Ecole Normale Supérieure, Centre National de la Recherche Scientifique/ UMR 8197, Paris, France.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Saulière
authors.forename: Nazmul
authors.initials: N
authors.lastname: Haque
authors.forename: Scot
authors.initials: S
authors.lastname: Harms
authors.forename: Isabelle
authors.initials: I
authors.lastname: Barbosa
authors.forename: Marco
authors.initials: M
authors.lastname: Blanchette
authors.forename: Hervé
authors.initials: H
authors.lastname: Le Hir
mesh_terms: D020413:3' Untranslated Regions; D000818:Animals; D002460:Cell Line; D018388:Codon, Terminator; D029721:Drosophila Proteins / Q000378:metabolism; D004331:Drosophila melanogaster / Q000166:cytology / Q000235:genetics*; D039601:Eukaryotic Initiation Factor-4A / Q000378:metabolism; D005091:Exons / Q000235:genetics*; D017930:Genes, Reporter; D007438:Introns / Q000235:genetics; D049409:Luciferases, Firefly / Q000235:genetics; D049410:Luciferases, Renilla / Q000235:genetics; D008957:Models, Genetic; D046912:Multiprotein Complexes; D009687:Nuclear Proteins / Q000378:metabolism; D022821:RNA Splice Sites; D012326:RNA Splicing; D020871:RNA Stability; D012333:RNA, Messenger / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000378:metabolism
chemical_list: D020413:3' Untranslated Regions; D018388:Codon, Terminator; D029721:Drosophila Proteins; C107372:Mago protein, Drosophila; D046912:Multiprotein Complexes; D009687:Nuclear Proteins; D022821:RNA Splice Sites; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; C440016:tsu protein, Drosophila; D049410:Luciferases, Renilla; D049409:Luciferases, Firefly; D039601:Eukaryotic Initiation Factor-4A
references.citation: Mol Cell. 2003 Apr;11(4):939-49
references.pmid: 12718880
references.citation: Nat Rev Genet. 2008 Sep;9(9):699-712
references.pmid: 18679436
references.citation: FEBS Lett. 2007 Jun 19;581(15):2845-53
references.pmid: 17531985
references.citation: Curr Opin Cell Biol. 2009 Jun;21(3):394-402
references.pmid: 19359157
references.citation: Mol Cell. 2009 Aug 28;35(4):479-89
references.pmid: 19716792
references.citation: EMBO J. 2000 Dec 15;19(24):6860-9
references.pmid: 11118221
references.citation: Cell. 2009 Feb 20;136(4):688-700
references.pmid: 19239889
references.citation: EMBO J. 2008 Jun 4;27(11):1585-95
references.pmid: 18451801
references.citation: EMBO J. 2003 Aug 1;22(15):3960-70
references.pmid: 12881430
references.citation: Nat Genet. 2004 Oct;36(10):1073-8
references.pmid: 15448691
references.citation: Nature. 2004 Apr 29;428(6986):959-63
references.pmid: 15118729
references.citation: Nat Struct Mol Biol. 2008 Jan;15(1):85-93
references.pmid: 18066079
references.citation: Mol Cell Biol. 1998 Sep;18(9):5272-83
references.pmid: 9710612
references.citation: RNA. 2005 Oct;11(10):1530-44
references.pmid: 16199763
references.citation: Nat Struct Mol Biol. 2009 Feb;16(2):107-13
references.pmid: 19190664
references.citation: Mol Cell Biol. 2009 Jan;29(1):281-301
references.pmid: 18981222
references.citation: Semin Cell Dev Biol. 2007 Apr;18(2):186-93
references.pmid: 17459736
references.citation: Genes Dev. 2006 Feb 1;20(3):355-67
references.pmid: 16452507
references.citation: Mol Cell Biol. 2009 Jul;29(13):3517-28
references.pmid: 19414594
references.citation: Curr Opin Struct Biol. 2008 Feb;18(1):112-9
references.pmid: 18164611