Assessment of isorhamnetin 3-O-neohesperidoside from Acacia salicina: protective effects toward oxidation damage and genotoxicity induced by aflatoxin B1 and nifuroxazide.
Antioxidant activity of isorhamnetin 3-O-neohesperidoside, isolated from the leaves of Acacia salicina, was determined by the ability of this compound to inhibit xanthine oxidase activity and to scavenge the free radical 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS(.-)) diammonium salt. Antigenotoxic activity was assessed using the SOS chromotest assay. This compound has the ability to scavenge the ABTS(.+) radical by a hydrogen donating mechanism. We also envisaged the study of the antioxidant effect of this compound by the enzymatic xanthine/xanthine oxidase (X/XOD) assay. Results indicated that isorhamnetin 3-O-neohesperidoside was a potent inhibitor of xanthine oxidase and superoxide anion scavengers. Moreover, this compound induced an inhibitory activity against nifuroxazide and aflatoxine B1 (AFB1) induced genotoxicity. Taken together, these observations provide evidence that isorhamnetin 3-O-neohesperidoside isolated from the leaves of A. salicina is able to protect cells against the consequences of oxidative stress.
Bouhlel
Ines
I
Unité de Pharmacognosie/Biologie Moléculaire 99/UR/07-03. Faculté de Pharmacie de Monastir, Rue Avicenne 5000 Monastir, Tunisia.
Limem
Ilef
I
Skandrani
Ines
I
Nefatti
Aicha
A
Ghedira
Kamel
K
Dijoux-Franca
Marie-Genevieve
MG
Leila
Chekir-Ghedira
CG
D000045:Acacia / Q000737:chemistry*; D016604:Aflatoxin B1 / Q000633:toxicity*; D000818:Animals; D016587:Antimutagenic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D045643:DNA Repair Enzymes / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D044948:Flavonols / Q000302:isolation & purification / Q000494:pharmacology; D005609:Free Radicals / Q000737:chemistry; D062385:Hydroxybenzoates / Q000633:toxicity*; D008862:Microsomes, Liver / Q000187:drug effects; D009152:Mutagenicity Tests; D009581:Nitrofurans / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D051381:Rats; D014969:Xanthine Oxidase / Q000037:antagonists & inhibitors
D016587:Antimutagenic Agents; D000975:Antioxidants; D044948:Flavonols; D005609:Free Radicals; D062385:Hydroxybenzoates; D009581:Nitrofurans; D010936:Plant Extracts; C469309:isorhamnetin-3-O-neohesperidoside; D016604:Aflatoxin B1; D014969:Xanthine Oxidase; D045643:DNA Repair Enzymes; C013150:nifuroxazide