title: Appraisal of translocation pathways for displaying ankyrin repeat protein on phage particles.
abstract: Depending on the molecular properties of the proteins of interest (POI), the rate of success in displaying proteins on phage particles is unpredictable. Formation of polypeptide tertiary structure in the cytoplasm occasionally results in low level display on viral particles. Here we assessed the influence of different leader peptides on the display of a premature cytoplasmic folding protein, ankyrin repeat protein (ARP), via the minor coat protein pIII. These peptides include the Sec, SRP and Tat pathways. The results demonstrated that the Sec and SRP pathways were capable of displaying the protein on the viral particle, whereas the Tat pathway failed to do so. Interestingly, the Tat pathway efficiently directed ARP through its translocon without fusing with pIII. Furthermore, the soluble form of ARP was detected in Escherichia coli periplasm.
authors.affiliation: Division of Clinical Immunology, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand. sawitree2727@hotmail.com
authors.forename: Sawitree
authors.initials: S
authors.lastname: Nangola
authors.forename: Philippe
authors.initials: P
authors.lastname: Minard
authors.forename: Chatchai
authors.initials: C
authors.lastname: Tayapiwatana
mesh_terms: D000595:Amino Acid Sequence; D017089:Ankyrin Repeat; D017487:Ankyrins / Q000737:chemistry / Q000378:metabolism*; D001435:Bacteriophages / Q000378:metabolism; D004926:Escherichia coli / Q000166:cytology / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D021381:Protein Transport; D018271:Signal Recognition Particle; D014771:Virion / Q000378:metabolism
chemical_list: D017487:Ankyrins; D018271:Signal Recognition Particle