Multinucleated giant cells with an osteoclast phenotype derived from caprine peripheral blood mononuclear cells.
Formation of multinucleated giant cells (MGCs) by macrophage fusion is a typical cytopathic effect of lentiviral replication in caprine monocytes and MGC formation from cultured caprine peripheral blood mononuclear cells (PBMCs) has been considered to be diagnostic for small ruminant lentivirus (SRLV) infection. In this study, formation of MGCs was observed after 7-14 days when PBMCs were cultured from healthy goats free from SRLV infection. These MGCs expressed tartrate-resistant acid phosphatase, calcitonin receptor, integrin αVβ3, cathepsin K and matrix metalloproteinase 9 and were able to resorb bone in vitro in the absence of RANKL and macrophage colony stimulating factor, consistent with an osteoclast phenotype.
Pisoni
Giuliano
G
Università degli Studi di Milano, Dipartimento di Patologia Animale, Igiene e Sanità Pubblica Veterinaria, via Celoria 10, 20133 Milano, Italy. giuliano.pisoni@unimi.it
D'Amelio
Patrizia
P
Sassi
Francesca
F
Manarolla
Giovanni
G
Scaccabarozzi
Licia
L
Locatelli
Clara
C
Mazzocchi
Nausicaa
N
Baruscotti
Mirko
M
DiFrancesco
Dario
D
Moroni
Paolo
P
D000135:Acid Phosphatase / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001842:Bone and Bones / Q000378:metabolism; D002478:Cells, Cultured; D005786:Gene Expression Regulation; D015726:Giant Cells / Q000473:pathology*; D006041:Goats; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D016173:Macrophage Colony-Stimulating Factor / Q000235:genetics / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D009000:Monocytes / Q000378:metabolism*; D010010:Osteoclasts / Q000166:cytology* / Q000378:metabolism / Q000473:pathology; D010447:Peptide Hydrolases / Q000235:genetics / Q000378:metabolism; D010641:Phenotype; D053245:RANK Ligand / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D000071681:Tartrate-Resistant Acid Phosphatase
D007527:Isoenzymes; D008565:Membrane Proteins; D053245:RANK Ligand; D016173:Macrophage Colony-Stimulating Factor; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase; D010447:Peptide Hydrolases