Inhibitory effect of the essential oil from Chamaecyparis obtusa on the growth of food-borne pathogens.
In this study, the antibacterial activity of essential oil from Chamaecyparis obtusa (Sieb. et Zucc) leaves and twigs was investigated. The test strains were Klebsiella pneumoniae, Listeria monocytogenes, Salmonella typhimurium, Staphylococcus aureus, Escherichia coli, Legionella pneumophila, and Methicilline-resistant Staphylococcus aureus. Antibacterial activity was estimated by measuring bacterial growth inhibition. Histopathological examination was also performed. C. obtusa oil distinctly inhibited the growth of all test strains and exhibited the strongest antibacterial activity against L. monocytogenes. It was chromatographically divided into several fractions. The fractions were further tested against antibacterial activity and their chemical compositions were analyzed. The fraction containing terpinen-4-ol (TA) showed high antibacterial activity toward all strains tested. Tests with authentic samples showed that TA played a major role in the antibacterial activity of C. obtusa oil, and in a mice test, the oil actively minimized inflammation by S. aureus.
Park
Mi-Jin
MJ
Division of Green Business Management, Department of Forest Resources Utilization, Korea Forest Research Institute, Seoul, Republic of Korea.
Choi
Won-Sil
WS
Kang
Ha-Young
HY
Gwak
Ki-Seob
KS
Lee
Geun-Shik
GS
Jeung
Eui-Bae
EB
Choi
In-Gyu
IG
D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects / Q000254:growth & development*; D029780:Chamaecyparis / Q000737:chemistry*; D005260:Female; D005516:Food Microbiology; D005517:Foodborne Diseases / Q000188:drug therapy / Q000382:microbiology*; D006131:Growth Inhibitors / Q000494:pharmacology*; D006801:Humans; D051379:Mice; D008813:Mice, Inbred ICR; D009822:Oils, Volatile / Q000032:analysis / Q000494:pharmacology*; D010938:Plant Oils / Q000032:analysis / Q000494:pharmacology*; D013729:Terpenes / Q000032:analysis / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D006131:Growth Inhibitors; D009822:Oils, Volatile; D010938:Plant Oils; D013729:Terpenes; C034019:terpinenol-4
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