[Sequence analysis of rDNA-ITS of Bai Shouwu from different species].
OBJECTIVE
To study the genetic diversity of rDNA ITS sequences in different species of Bai Shouwu, utilize the molecular diversity of ITS sequences to authenticate the different species of Bai Shouwu.


METHODS
Firstly, total DNA was extracted from the different species of Bai Shouwu. Secondly, the ITS sequence was amplified by PCR with universal primer of ITS and sequenced after cloning and purification.


RESULTS
From four species the complete sequence of ITS and 5.8 S rDNA, the partial sequences of 18S rDNA and 26S rDNA were obtained. The rDNA ITS sequences of Cynanchum bungei (sign in No. GU198970 and No. GU479037) were obtained. Ten variable sites among the sequences were found.


CONCLUSIONS
ITS sequence could be used to authenticate the species. The method could be used to identify germplasm resources and authenticate.
Zhang
Ning
N
Faculty of Pharmacy, Shandong University of Trditional Chinese Medicine, Ji'nan 250355, China. zhangning301@163.com
Yan
Bin
B
Xu
Xinghang
X
Xu
Lingchuan
L
D031105:Cynanchum / Q000145:classification / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics*; D008969:Molecular Sequence Data
D018744:DNA, Plant; D021903:DNA, Ribosomal Spacer