title: A sperm viability test using SYBR-14/propidium iodide flow cytometry as a tool for rapid screening of primary ciliary dyskinesia patients and for choosing sperm sources for intracytoplasmic sperm injection.
abstract: Spermatozoa viability tests based on dual-color flow cytometry after staining with Sybr-14/propidium iodide were performed on 44 men with complete asthenospermia for primary ciliary dyskinesia (PCD) screening, and seven were identified with PCD by electron microscopy of ultrastructural ciliary defects. Six PCD patients underwent eight intracytoplasmic sperm injection therapy cycles using ejaculated sperm or testicular sperm, obtaining a mean fertilization rate of 46.6%, with three healthy babies born and one in utero at the time of writing.
authors.affiliation: Department of Gynecology and Obstetrics, Medical School of Fudan University, and Reproductive Medical Center, Ruijin Hospital Affiliated to Medical School of Shanghai Jiaotong University, Shanghai, People's Republic of China.
authors.forename: Zhi-hong
authors.initials: ZH
authors.lastname: Niu
authors.forename: Xue-feng
authors.initials: XF
authors.lastname: Huang
authors.forename: Xiao-feng
authors.initials: XF
authors.lastname: Jia
authors.forename: Jufen
authors.initials: J
authors.lastname: Zheng
authors.forename: Yao
authors.initials: Y
authors.lastname: Yuan
authors.forename: Ting-yan
authors.initials: TY
authors.lastname: Shi
authors.forename: Hua
authors.initials: H
authors.lastname: Diao
authors.forename: He-guo
authors.initials: HG
authors.lastname: Yu
authors.forename: Fei
authors.initials: F
authors.lastname: Sun
authors.forename: Hui-qing
authors.initials: HQ
authors.lastname: Zhang
authors.forename: Hui-juan
authors.initials: HJ
authors.lastname: Shi
authors.forename: Yun
authors.initials: Y
authors.lastname: Feng
mesh_terms: D000328:Adult; D053627:Asthenozoospermia / Q000175:diagnosis* / Q000209:etiology / Q000628:therapy*; D002470:Cell Survival; D002925:Ciliary Motility Disorders / Q000150:complications / Q000175:diagnosis*; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D008297:Male; D008403:Mass Screening / Q000379:methods; D009930:Organic Chemicals; D011419:Propidium; D020554:Sperm Injections, Intracytoplasmic; D013094:Spermatozoa / Q000166:cytology*
chemical_list: D009930:Organic Chemicals; C093207:SYBR-14; D011419:Propidium