Metabolically engineered male sterility in rapeseed (Brassica napus L.).
Male sterility is of special interest as a mechanism allowing hybrid breeding, especially in important crops such as rapeseed (Brassica napus). Male sterile plants are also suggested to be used as a biological safety method to prevent the spread of transgenes, a risk that is high in the case of rapeseed due to the mode of pollination, out-crossing by wind or insects, and the presence of related, cross-pollinating species in the surrounding ecosystem in Europe. Different natural occurring male sterilities and alloplasmic forms have been tried to be used in rapeseed with more or less success. Due to the difficulties and limitations with these systems, we present a biotechnological alternative: a metabolically engineered male sterility caused by interference with anther-specific cell wall-bound invertase. This is an essential enzyme for carbohydrate supply of the symplastically isolated pollen. The activity of this enzyme is reduced either by antisense interference or by expressing an invertase inhibitor under control of the anther-specific promoter of the invertase with the consequence of a strong decrease of pollen germination ability.
Engelke
Thomas
T
Lehrstuhl für Pharmazeutische Biologie, Julius von Sachs Institut, Universität Würzburg, Julius von Sachs Platz 2, 97082, Würzburg, Germany. thomas.engelke@biozentrum.uni-wuerzburg.de
Hirsche
J
J
Roitsch
T
T
D017360:Arabidopsis / Q000187:drug effects / Q000201:enzymology / Q000235:genetics; D029688:Brassica napus / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant / Q000235:genetics; D017930:Genes, Reporter; D005818:Genetic Engineering / Q000379:methods*; D018525:Germination / Q000187:drug effects; D005966:Glucuronidase / Q000378:metabolism; D005810:Multigene Family / Q000235:genetics; D009928:Organ Specificity / Q000187:drug effects / Q000235:genetics; D010802:Phylogeny; D051479:Plant Infertility / Q000187:drug effects / Q000235:genetics*; D011058:Pollen / Q000187:drug effects / Q000254:growth & development; D011401:Promoter Regions, Genetic / Q000235:genetics; D034622:RNA Interference / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D013045:Species Specificity; D043324:beta-Fructofuranosidase / Q000037:antagonists & inhibitors / Q000235:genetics
D004791:Enzyme Inhibitors; D012333:RNA, Messenger; D043324:beta-Fructofuranosidase; D005966:Glucuronidase
Plant J. 2007 Nov;52(3):570-82
17764500
Plant J. 1999 Dec;20(6):707-11
10652142
J Exp Bot. 2005 Jan;56(409):179-90
15533880
J Mol Evol. 2005 May;60(5):615-34
15983871
Planta. 2007 Apr;225(5):1193-203
17086397
Plant Physiol. 1999 Sep;121(1):1-8
10482654
Gene. 1997 Sep 15;197(1-2):239-51
9332372
Plant Cell Rep. 2003 Feb;21(6):599-604
12789436
Plant J. 2003 Dec;36(5):697-710
14617070
Theor Appl Genet. 2009 Jan;118(2):235-45
18825361
Nucleic Acids Res. 1993 Apr 25;21(8):1903-9
8388101
Plant Mol Biol. 1999 Dec;41(6):741-51
10737139
Bioinformatics. 2007 Nov 1;23(21):2947-8
17846036
Planta. 1998 Dec;207(2):259-65
9951726
Plant J. 2003 Jul;35(2):262-72
12848830
Plant Cell Rep. 2005 Jun;24(4):225-36
15759120
Theor Appl Genet. 2008 Feb;116(3):355-62
18034224
Planta. 2008 Mar;227(4):795-807
18034262
Plant Physiol. 2003 Feb;131(2):603-9
12586884
Trends Plant Sci. 1999 Oct;4(10):401-407
10498964
J Exp Bot. 2003 Jan;54(382):525-31
12508063
Theor Appl Genet. 2006 Dec;114(1):93-9
17036218
Curr Genet. 1997 Apr;31(4):325-35
9108140
Mol Gen Genet. 1992 Nov;235(2-3):340-8
1281515
Proc Natl Acad Sci U S A. 2001 May 22;98(11):6522-7
11371651
Theor Appl Genet. 1983 May;65(3):231-8
24263420
Plant Physiol. 1995 May;108(1):285-94
7784506
Adv Bioinformatics. 2008;2008:420747
19956698
Nucleic Acids Res. 1997 Sep 1;25(17):3389-402
9254694
Proc Natl Acad Sci U S A. 1998 Aug 18;95(17):10032-7
9707595
Plant Physiol. 1997 Sep;115(1):273-82
9306701
Plant Physiol. 2007 Nov;145(3):616-25
17873089
Mol Gen Genet. 1994 Jun 3;243(5):540-7
8208245
Plant Mol Biol. 1995 Apr;28(1):189-94
7787183
Planta. 2003 Apr;216(6):951-60
12687362
Plant Cell. 1991 Dec;3(12):1349-62
1840901
J Exp Bot. 2010 Jun;61(10):2693-706
20427415