Improved performance of the modified Hodge test with MacConkey agar for screening carbapenemase-producing Gram-negative bacilli.
The detection of carbapenemases in Gram-negative bacilli is important for optimal patient treatment and to control spread of the resistance. The modified Hodge test can detect carbapenemase-producing Gram-negative bacilli. In this study, we compared the performance of MacConkey agar and Mueller-Hinton agar for metallo-β-lactamase (MBL) and OXA carbapenemase screening. Overall, the performance of Hodge test was better with MacConkey agar due to enhanced release of β-lactamases from the cells in the presence of bile compounds. Concomitant use of the modified Hodge test could resolve most of the problems with uncertain double-disk synergy tests in MBL detection.
Lee
Kyungwon
K
Department of Laboratory Medicine, Yonsei University College of Medicine, Seoul, South Korea.
Kim
Chang Ki
CK
Yong
Dongeun
D
Jeong
Seok Hoon
SH
Yum
Jong Hwa
JH
Seo
Young Hee
YH
Docquier
Jean-Denis
JD
Chong
Yunsop
Y
D000362:Agar; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000096:biosynthesis*; D003470:Culture Media / Q000737:chemistry; D000077727:Ertapenem; D006090:Gram-Negative Bacteria / Q000187:drug effects / Q000201:enzymology*; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology*; D006801:Humans; D015378:Imipenem / Q000494:pharmacology; D008403:Mass Screening / Q000379:methods*; D008826:Microbial Sensitivity Tests / Q000379:methods; D001618:beta-Lactamases / Q000096:biosynthesis*; D047090:beta-Lactams / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D003470:Culture Media; D047090:beta-Lactams; D015378:Imipenem; D000362:Agar; D001618:beta-Lactamases; C063912:carbapenemase; D000077727:Ertapenem