Isolation and characterization of a gene encoding a polyethylene glycol-induced cysteine protease in common wheat.
Plant cysteine protease (CP) genes are induced by abiotic stresses such as drought, yet their functions remain largely unknown. We isolated the full-length cDNA encoding a Triticum aestivum CP gene, designated TaCP, from wheat by the rapid amplification of cDNA ends (RACE) method. Sequence analysis revealed that TaCP contains an open reading frame encoding a protein of 362 amino acids, which is 96% identical to barley cysteine protease HvSF42. The TaCP transcript level in wheat seedlings was upregulated during polyethylene glycol (PEG) stress, with a peak appearing around 12 h after treatment. TaCP expression level increased rapidly with NaCl treatment at 48 h. TaCP responded strongly to low temperature (4 degree C) treatment from 1 h post-treatment and reached a peak of about 40-fold at 72 h. However, it showed only a very slight response to abscisic acid (ABA). More than one copy of TaCP was present in each of the three genomes of hexaploid wheat and its diploid donors. TaCP fused with green fluorescent protein (GFP) was located in the plasma membrane of onion epidermis cells. Transgenic Arabidopsis plants overexpressing TaCP showed stronger drought tolerance and higher CP activity under water-stressed conditions than wild-type Arabidopsis plants. The results suggest that TaCP plays a role in tolerance to water deficit.
Zang
Qing-Wei
QW
The National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Crop Germplasm and Biotechnology, Ministry of Agriculture, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Wang
Cai-Xiang
CX
Li
Xu-Yan
XY
Guo
Zhi-Ai
ZA
Jing
Rui-Lian
RL
Zhao
Jun
J
Chang
Xiao-Ping
XP
D000595:Amino Acid Sequence; D017360:Arabidopsis; D003001:Cloning, Molecular; D057056:Cysteine Proteases / Q000235:genetics* / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics; D003681:Dehydration / Q000201:enzymology*; D055864:Droughts; D018628:Gene Dosage; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000378:metabolism; D011092:Polyethylene Glycols; D036226:Seedlings / Q000378:metabolism; D017422:Sequence Analysis, DNA; D014908:Triticum / Q000201:enzymology / Q000235:genetics*
D018076:DNA, Complementary; D010940:Plant Proteins; D011092:Polyethylene Glycols; D057056:Cysteine Proteases
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