Mixtures of Uncaria and Tabebuia extracts are potentially chemopreventive in CBA/Ca mice: a long-term experiment.
A long-term experimental animal model was developed by our research group for the evaluation of potential chemopreventive effects. The inhibitory effects of agents on carcinogen (7,12-dimethylbenz[a]anthracene (DMBA) induced molecular epidemiological biomarkers, in this case the expression of key onco/suppressor genes were investigated. The expression pattern of c-myc, Ha-ras, Bcl-2, K-ras protooncogene and p53 tumour suppressor gene were studied to elucidate early carcinogenic and potential chemopreventive effects. The consumption of so-called Claw of Dragon tea (CoD™ tea) containing the bark of Uncaria guianensis, Cat's Claw (Uncaria sp. U. tomentosa) and Palmer trumpet-tree (Tabebuia sp. T. avellanedae) was able to decrease the DMBA-induced onco/suppressor gene overexpression in a short-term animal experiment. In a following study CBA/Ca mice were treated with 20 mg/kg bw DMBA intraperitoneally (i.p.) and the expression patterns of onco/suppressor genes were examined at several time intervals. According to the examined gene expression patterns in this long-term experiment the chemopreventive effect of CoD™ tea consumption could be confirmed.
Budán
Ferenc
F
Institute of Public Health, Medical School, University of Pécs, Szigeti str. 12, H-7643 Pécs, Hungary. budfer2@gmail.com
Szabó
István
I
Varjas
Tímea
T
Nowrasteh
Ghodratollah
G
Dávid
Tamás
T
Gergely
Péter
P
Varga
Zsuzsa
Z
Molnár
Kornélia
K
Kádár
Balázs
B
Orsós
Zsuzsa
Z
Kiss
István
I
Ember
István
I
D015127:9,10-Dimethyl-1,2-benzanthracene / Q000037:antagonists & inhibitors / Q000633:toxicity; D000818:Animals; D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D005260:Female; D005786:Gene Expression Regulation; D016147:Genes, Tumor Suppressor; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D010936:Plant Extracts / Q000494:pharmacology*; D029663:Tabebuia / Q000737:chemistry*; D032070:Uncaria / Q000737:chemistry*
D016588:Anticarcinogenic Agents; D010936:Plant Extracts; D015127:9,10-Dimethyl-1,2-benzanthracene