[Screening and characterization of peptides specifically binding to the schistosomulum tegument of Schistosoma japonicum].
OBJECTIVE
To screen and analyze the peptides in 12 phage-display peptide library specifically binding to the schistosomulum tegument of Schistosoma japonicum.


METHODS
A 12 phage-display peptide library was screened with the S. japonicum schistosomula as the target cells for biopanning by degrees, positive clones picked randomly were deduced by DNA sequencing. According the sequence seeing result, immunohistochemical staining was performed to determine the specificity of the phages to the tegument. To test their targeting efficacy, the interested phage clones were infused back to the mice infected with S. japonicum, mice were sacrificed 2.5 hours later, and the phage distribution in the liver and the tegument of schistosomula was appraised, respectively.


RESULTS
After 3 rounds of biopanning, the phage recovery rate increased from 0.77 x 10(-8) to 0.75 x 10(-5), indicating that the phage library was successfully enriched in the tegument of schistosomula. Seventy-five percent (15/20) of the analyzed sequences were identical with a sequence of QHPRIRKOOOOO. The immunohistochemical stainings showed this sequence specifically binding to the tegument. In vivo titering displayed that this sequence selectively targeted the tegument.


CONCLUSIONS
The peptide of QHPRIRKOOOOO specifically binds to the schistosomulum tegument.
Wei
Qi
Q
Department of Immunology, Xiangya Medical School, the Central South University, Changsha 410013, China.
Liu
Yan
Y
Zeng
Qing-Ren
QR
Chen
Yu-Xiao
YX
Zhou
Jun
J
Liu
Bi-Yuan
BY
Zou
Yan
Y
Yang
Sheng-Hui
SH
Cai
Li-Ting
LT
Li
Li-Xin
LX
Lan
Ling-Mei
LM
D000818:Animals; D007814:Larva; D051379:Mice; D008815:Mice, Inbred Strains; D019151:Peptide Library; D010455:Peptides / Q000235:genetics / Q000302:isolation & purification*; D011817:Rabbits; D012549:Schistosoma japonicum / Q000302:isolation & purification*; D017422:Sequence Analysis, DNA
D019151:Peptide Library; D010455:Peptides