Characterization of nucleosides and nucleobases in fruits of Ziziphus jujuba by UPLC-DAD-MS.
The fruit of Ziziphus jujuba , named dazao in Chinese, has been utilized as food as well as crude drugs in China for thousands of years. To explore the profiles of the nucleosides and nucleobases in this fruit, an ultraperformance liquid chromatograph coupled with a photodiode array detector and electrospray ionization-mass spectrometer method (UPLC-DAD-MS) has been established and validated in this paper. The validated method was successfully applied for the simultaneous characterization and quantitation of 9 nucleosides and nucleobases in 49 dazao samples, which comprised 43 cultivars from 26 cultivation regions. Furthermore, principal component analysis (PCA) was performed to classify the samples on the basis of the contents of the nine analyzed compounds. The results showed that almost all of these dazao samples were rich in nucleosides and nucleobases, although their contents were obviously various, and the proposed method could serve as a prerequisite for quality control of jujube products.
Guo
Sheng
S
Jiangsu Key Laboratory for TCM Formulae Research, Nanjing University of Chinese Medicine, Nanjing 210046, People's Republic of China.
Duan
Jin-Ao
JA
Tang
Yu-Ping
YP
Zhu
Zhen-Hua
ZH
Qian
Ye-Fei
YF
Yang
Nian-Yun
NY
Shang
Er-Xin
EX
Qian
Da-Wei
DW
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D000242:Cyclic AMP / Q000032:analysis; D006152:Cyclic GMP / Q000032:analysis; D003890:Desiccation; D005638:Fruit / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods*; D009705:Nucleosides / Q000032:analysis*; D009711:Nucleotides / Q000032:analysis*; D010936:Plant Extracts / Q000737:chemistry; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry; D031957:Ziziphus / Q000737:chemistry*
D009705:Nucleosides; D009711:Nucleotides; D010936:Plant Extracts; D000242:Cyclic AMP; D006152:Cyclic GMP