Preparation of a novel glycidyl methacrylate-based monolith and its application for the determination of m-nisoldipine in human plasma.
A new method has been developed for preparing monolithic materials by polymerisation of the oil-in-water (O/W) emulsions with nonion-surfactant (Pluronic F68). Morphology of monolithic materials is studied by scanning electron microscopy. The properties of the column are investigated, and the column exhibits the ability of low backpressure and fast analysis. Using this monolithic column, on-line sample clean-up and screening of m-nisoldipine in human plasma samples have been investigated. Chromatography is performed by reversed-phase high-performance liquid chromatography (RP-HPLC) on a C(18) column with UV detection at 237 nm. The linear range of m-nisoldipine in human plasma is 2-200 ng/mL (r = 0.9992, n = 7). And the limit of detection is 1.5 ng/mL. The 12-h pharmacokinetic profile of m-nisoldipine in mice after oral administration has been investigated. The results indicate that the method could be used for monitoring of m-nisoldipine and enabled simple and rapid assay of the drugs in human plasma.
Zhang
Qiaoxia
Q
Key Laboratory for Drug Quality Control and Analysis of Hebei Province, College of Pharmacy, Hebei University, Baoding 071002, China.
Yang
Gengliang
G
Liu
Haiyan
H
Yang
Jing
J
Yan
Yongna
Y
Bai
Ligai
L
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D004655:Emulsions; D004852:Epoxy Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D006801:Humans; D016014:Linear Models; D008297:Male; D008689:Methacrylates / Q000138:chemical synthesis / Q000737:chemistry*; D051379:Mice; D008855:Microscopy, Electron, Scanning; D015737:Nisoldipine / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D017550:Spectroscopy, Fourier Transform Infrared
D004655:Emulsions; D004852:Epoxy Compounds; D008689:Methacrylates; D015737:Nisoldipine; C007870:glycidyl methacrylate