title: Succinic acid production by Actinobacillus succinogenes using hydrolysates of spent yeast cells and corn fiber.
abstract: The enzymatic hydrolysate of spent yeast cells was evaluated as a nitrogen source for succinic acid production by Actinobacillus succinogenes NJ113, using corn fiber hydrolysate as a carbon source. When spent yeast cell hydrolysate was used directly as a nitrogen source, a maximum succinic acid concentration of 35.5 g/l was obtained from a glucose concentration of 50 g/l, with a glucose utilization of 95.2%. Supplementation with individual vitamins showed that biotin was the most likely factor to be limiting for succinic acid production with spent yeast cell hydrolysate. After supplementing spent yeast cell hydrolysate and 90 g/l of glucose with 150 μg/l of biotin, cell growth increased 32.5%, glucose utilization increased 37.6%, and succinic acid concentration was enhanced 49.0%. As a result, when biotin-supplemented spent yeast cell hydrolysate was used with corn fiber hydrolysate, a succinic acid yield of 67.7% was obtained from 70.3 g/l of total sugar concentration, with a productivity of 0.63 g/(l h). Our results suggest that biotin-supplemented spent yeast cell hydrolysate may be an alternative nitrogen source for the efficient production of succinic acid by A. succinogenes NJ113, using renewable resources.
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, People's Republic of China.
authors.forename: Ke-Quan
authors.initials: KQ
authors.lastname: Chen
authors.forename: Jian
authors.initials: J
authors.lastname: Li
authors.forename: Jiang-Feng
authors.initials: JF
authors.lastname: Ma
authors.forename: Min
authors.initials: M
authors.lastname: Jiang
authors.forename: Ping
authors.initials: P
authors.lastname: Wei
authors.forename: Zhong-Min
authors.initials: ZM
authors.lastname: Liu
authors.forename: Han-Jie
authors.initials: HJ
authors.lastname: Ying
mesh_terms: D000188:Actinobacillus / Q000187:drug effects / Q000378:metabolism*; D001710:Biotin / Q000494:pharmacology; D002244:Carbon / Q000494:pharmacology; D004043:Dietary Fiber / Q000378:metabolism*; D005285:Fermentation / Q000187:drug effects; D005947:Glucose / Q000494:pharmacology; D006868:Hydrolysis / Q000187:drug effects; D009584:Nitrogen / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D019802:Succinic Acid / Q000138:chemical synthesis*; D014815:Vitamins / Q000494:pharmacology; D014866:Waste Products / Q000032:analysis*; D003313:Zea mays / Q000187:drug effects / Q000378:metabolism*
chemical_list: D004043:Dietary Fiber; D014815:Vitamins; D014866:Waste Products; D001710:Biotin; D002244:Carbon; D019802:Succinic Acid; D005947:Glucose; D009584:Nitrogen