Inulin prolongs survival of intragastrically administered Lactobacillus plantarum No. 14 in the gut of mice fed a high-fat diet.
We tested whether a high-fat diet (HFD) impairs the survival of probiotics in mice. In Expt. 1, after feeding either a HFD (62.7% energy) or a normal-fat diet (NFD; 11.1% energy) for 2 d, C57BL/6 mice were i.g. administered Lactobacillus plantarum No. 14. Fecal recovery of viable L. plantarum was significantly decreased 99% by the HFD compared with the NFD. Total bile acid concentrations in the small intestine and cecum were significantly higher (1.5- and 2.2-fold of NFD, respectively) in mice fed HFD than in those fed NFD. Cholic acid and deoxycholic acid significantly reduced the viability of L. plantarum No. 14 in culture experiments. In Expt. 2, after feeding HFD for 2 d, simultaneous administration of inulin (10 mg) with L. plantarum No. 14 significantly increased (100-fold of that without inulin) the fecal recovery of viable L. plantarum. Inulin administration did not alter intestinal bile acid concentrations. In Expt. 3, after feeding HFD for 2 d, mice were i.g. administered either inulin (10 mg) or vehicle and, after 6 h, cecal contents were subjected to culture experiments. Growth of L. plantarum No. 14 was significantly higher in the cecal contents of inulin-administered mice than vehicle-administered mice. Inulin supplementation to cecal contents of vehicle-administered mice significantly enhanced the growth of L. plantarum No. 14. We propose that HFD impairs the survival of probiotics in the gut due to increased bile acid stress and that simultaneous administration of inulin prolongs the survival of probiotics in mice fed HFD.
Takemura
Naoki
N
Graduate School of Life Science, Hokkaido University, Sapporo 060-8589, Japan.
Hagio
Masahito
M
Ishizuka
Satoshi
S
Ito
Hiroyuki
H
Morita
Tatsuya
T
Sonoyama
Kei
K
D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis / Q000494:pharmacology; D001647:Bile Acids and Salts / Q000032:analysis; D002432:Cecum / Q000382:microbiology; D019826:Cholic Acid / Q000032:analysis / Q000494:pharmacology; D015169:Colony Count, Microbial; D003840:Deoxycholic Acid / Q000032:analysis / Q000494:pharmacology; D004041:Dietary Fats / Q000009:adverse effects*; D005243:Feces / Q000382:microbiology; D005260:Female; D005766:Gastrointestinal Contents / Q000737:chemistry / Q000382:microbiology; D007421:Intestine, Small / Q000382:microbiology; D007444:Inulin / Q000008:administration & dosage* / Q000378:metabolism; D007778:Lactobacillus / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification; D048191:Lactiplantibacillus plantarum / Q000187:drug effects / Q000254:growth & development* / Q000302:isolation & purification; D051379:Mice; D008810:Mice, Inbred C57BL; D050296:Microbial Viability; D009994:Osmolar Concentration; D019936:Probiotics / Q000008:administration & dosage* / Q000378:metabolism; D013997:Time Factors
D000900:Anti-Bacterial Agents; D001647:Bile Acids and Salts; D004041:Dietary Fats; D003840:Deoxycholic Acid; D007444:Inulin; D019826:Cholic Acid