title: Plasticity of TRPM1 expression and localization in the wild type and degenerating mouse retina.
abstract: The light response in retinal ON bipolar cells is associated with disinhibition of current flow through cation channels recently identified as type 1 members of the melastatin transient receptor potential (TRPM) family. We determined the developmental expression of Trpm1 in the wild type C57BL/6, DBA/2J, DBA2J-Gpnmb mouse retinas and in Pde6brd1 retinas characterized by degeneration of rod photoreceptors. Trpm1 mRNA in wild type retinas was low at birth but exhibited progressive increases in abundance up to early adulthood at postnatal day 21 (P21). Retinal Trpm1 mRNA content did not decrease following loss of photoreceptors. At P21, TRPM1-immunopositive perikarya migrated into the outer nuclear layer. The TRPM1 protein was trafficked to discrete postsynaptic puncta in wild type retinas whereas in adult Pde6brd1 mouse retinas, TRPM1 translocated to bipolar perikarya and bar-like structures in the distal inner nuclear layer. These findings show that expression and localization of the TRPM1 in the mouse retina is plastic, modulated by use-dependence and availability of sustained excitatory input.
authors.affiliation: Department of Ophthalmology & Visual Sciences, John A. Moran Eye Center, Salt Lake City, UT 84132, United States. david.krizaj@hsc.utah.edu
authors.forename: David
authors.initials: D
authors.lastname: Križaj
authors.forename: Wei
authors.initials: W
authors.lastname: Huang
authors.forename: Takahisa
authors.initials: T
authors.lastname: Furukawa
authors.forename: Claudio
authors.initials: C
authors.lastname: Punzo
authors.forename: Wei
authors.initials: W
authors.lastname: Xing
mesh_terms: D000818:Animals; D051379:Mice; D008807:Mice, Inbred BALB C; D008811:Mice, Inbred DBA; D012333:RNA, Messenger / Q000378:metabolism; D012160:Retina / Q000378:metabolism*; D051245:Retinal Bipolar Cells / Q000378:metabolism* / Q000502:physiology; D012162:Retinal Degeneration / Q000235:genetics / Q000378:metabolism*; D050053:TRPM Cation Channels / Q000378:metabolism*; D014785:Vision, Ocular / Q000502:physiology*
chemical_list: D012333:RNA, Messenger; D050053:TRPM Cation Channels; C116297:Trpm1 protein, mouse
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