title: [The significance of Goodpasture antigen in hereditary nephritis].
abstract: BACKGROUND
  Two types of hereditary nephritis, nonprogressive and progressive, clinically present as asymptomatic haematuria, sometimes combined with proteinuria. At the onset, in both types, light microscopic changes are minimal, immunofluorescence findings are negative, and diagnosis can be made only upon electron microscopic findings that are considered to be specific.
  
  
  OBJECTIVE
  The aim of this study was to determine the significance of Goodpasture antigen detection in diagnosis of progressive and nonprogressive hereditary nephritis in its early phase.
  
  
  METHODS
  Analysis of renal biopsy specimens was done in patients with hereditary nephritis that were followed from 1990 to 2005. Progression of renal disease was examined in 14 patients with Alport's syndrome, 10 patients with thin basement membrane disease, and 6 patients with unclassified hereditary nephritis diagnosed. For all these cases, indirect immunofluorescence study with serum from a patient with high titer of Goodpasture autoantibodies that recognize the antigenic determinants in human glomerular and tubular basement membrane was performed.
  
  
  RESULTS
  In 11 out of 14 cases diagnosed as Alport's syndrome, there was negative staining with Goodpasture serum, and in 3 additional cases with Alport's syndrome, expression of Goodpasture antigen in glomerular basement membrane and thin basement membrane was highly reduced. In all 10 patients with thin basement membrane disease, immunofluorescence showed intensive, bright linear staining with Goodpasture serum along glomerular and tubular basement membrane. In 2 out of 6 patients with unclassified hereditary nephritis, Goodpasture antigen expression was very strong, in one patient it was very reduced, and in 3 patients it was negative.
  
  
  CONCLUSIONS
  The results of our study show that Goodpasture antigen detection plays a very important role in differential diagnosis of progressive and nonpregressive hereditary nephritis, particularly in early phases of the disease.
authors.forename: Gordana
authors.initials: G
authors.lastname: Basta-Jovanović
authors.forename: Sanja
authors.initials: S
authors.lastname: Radojević-Skodrić
authors.forename: Milena
authors.initials: M
authors.lastname: Jovanović
authors.forename: Ljiljana
authors.initials: L
authors.lastname: Bogdanović
authors.forename: Radovan
authors.initials: R
authors.lastname: Bogdanović
authors.forename: Visnja
authors.initials: V
authors.lastname: Lezaić
authors.forename: Vidosava
authors.initials: V
authors.lastname: Nesić
authors.forename: Steven
authors.initials: S
authors.lastname: Dikman
mesh_terms: D000293:Adolescent; D000328:Adult; D001324:Autoantigens / Q000032:analysis*; D002648:Child; D002675:Child, Preschool; D024141:Collagen Type IV / Q000032:analysis*; D005260:Female; D050533:Glomerular Basement Membrane / Q000276:immunology; D006801:Humans; D008297:Male; D008875:Middle Aged; D009394:Nephritis, Hereditary / Q000175:diagnosis / Q000276:immunology*; D055815:Young Adult
chemical_list: D001324:Autoantigens; D024141:Collagen Type IV; C057112:type IV collagen alpha3 chain