Peptidylarginine deiminase type 2 is over expressed in the glaucomatous optic nerve.
OBJECTIVE
To determine levels of Peptidyl arginine deiminase 2 (PAD2) and its product protein-bound citrulline in cadaver eyes that suffered from normal tension glaucoma (NTG) compared to primary open angle glaucoma (POAG), and controls.


METHODS
Western analysis, ELISA, and immunohistochemical analysis were performed with human tissues.


RESULTS
We report over expression of PAD2 and higher levels of its product protein-bound citrulline in the optic nerve of normal tension glaucoma patients (NTG).


CONCLUSIONS
This is the first report demonstrating that like in POAG, NTG also possesses elevated levels of both PAD2 and protein-bound citrulline.
Cafaro
Thamara A
TA
CIBICI, Faculty of Chemistry, National University of Córdoba, Argentina.
Santo
Stefanía
S
Robles
Lucena A
LA
Crim
Nicolás
N
Urrets-Zavalia
Julio A
JA
Serra
Horacio M
HM
D000368:Aged; D000369:Aged, 80 and over; D002956:Citrulline / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005901:Glaucoma / Q000201:enzymology* / Q000473:pathology*; D006801:Humans; D006867:Hydrolases / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009900:Optic Nerve / Q000201:enzymology* / Q000473:pathology*; D000080064:Protein-Arginine Deiminase Type 2; D000076342:Protein-Arginine Deiminases; D014019:Tissue Donors
D002956:Citrulline; D006867:Hydrolases; C000620352:PADI2 protein, human; D000080064:Protein-Arginine Deiminase Type 2; D000076342:Protein-Arginine Deiminases
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