title: IgG subclasses and DNA detection of HHV-6 and HHV-7 in healthy individuals.
abstract: Human herpesvirus 6 (HHV-6) and 7 (HHV-7) are common opportunistic agents in immunocompromised hosts, although infection with HHV-6 and HHV-7 can also be observed in immunocompetent hosts. Despite similar biology and epidemiology, this study evaluated differences in the IgG subclass distribution associated with HHV-6 and HHV-7 in seropositive, healthy persons. The identified subclasses were also compared with the detection of HHV-6 and HHV-7 DNA. For these assays, sera, plasma, and saliva samples were obtained from 40 healthy blood donors in Argentina who were seropositive for both HHV-6 and HHV-7. HHV-6 and HHV-7 DNA were detected in saliva and plasma samples using nested PCR, and specific IgG subclasses were determined using immunofluorescent assays of sera samples. HHV-7 DNA was detected in 90% of all plasma samples and in 100% of saliva samples. In contrast, HHV-6 DNA was not detected in any of the plasma samples, and it was detected in only 6 of 40 saliva samples. Determination of IgG subclass distributions showed that HHV-6 was restricted to IgG1, whereas HHV-7 IgG subclasses included two groups, one restricted only to IgG1 and the other to IgG1 and IgG3. These results demonstrate the differences between HHV-6 and HHV-7 DNA range detection in saliva and plasma samples, as well as the IgG subclass patterns for each virus type, in healthy persons in Argentina.
authors.affiliation: School of Medical Sciences. National University of Cordoba, Cordoba, Argentina.
authors.forename: Patricia
authors.initials: P
authors.lastname: Biganzoli
authors.forename: Leonardo
authors.initials: L
authors.lastname: Ferreyra
authors.forename: Paola
authors.initials: P
authors.lastname: Sicilia
authors.forename: Claudia
authors.initials: C
authors.lastname: Carabajal
authors.forename: Susana
authors.initials: S
authors.lastname: Frattari
authors.forename: Ana
authors.initials: A
authors.lastname: Littvik
authors.forename: Silvia
authors.initials: S
authors.lastname: Nates
authors.forename: Jorge
authors.initials: J
authors.lastname: Pavan
mesh_terms: D000293:Adolescent; D000328:Adult; D000914:Antibodies, Viral / Q000097:blood; D001118:Argentina / Q000453:epidemiology; D002353:Carrier State / Q000453:epidemiology* / Q000821:virology; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification*; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect / Q000379:methods; D015654:Herpesvirus 6, Human / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D016199:Herpesvirus 7, Human / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D008297:Male; D008875:Middle Aged; D010949:Plasma / Q000276:immunology / Q000821:virology; D016133:Polymerase Chain Reaction / Q000379:methods; D019349:Roseolovirus Infections / Q000453:epidemiology* / Q000821:virology; D012463:Saliva / Q000276:immunology / Q000821:virology; D044967:Serum / Q000276:immunology / Q000821:virology; D014773:Virology / Q000379:methods; D055815:Young Adult
chemical_list: D000914:Antibodies, Viral; D004279:DNA, Viral; D007074:Immunoglobulin G