title: Identification and characterization of an unusual class I myosin involved in vesicle traffic in Trypanosoma brucei.
abstract: Myosins are a multimember family of motor proteins with diverse functions in eukaryotic cells. African trypanosomes possess only two candidate myosins and thus represent a useful system for functional analysis of these motors. One of these candidates is an unusual class I myosin (TbMyo1) that is expressed at similar levels but organized differently during the life cycle of Trypanosoma brucei. This myosin localizes to the polarized endocytic pathway in bloodstream forms of the parasite. This organization is actin dependent. Knock down of TbMyo1 results in a significant reduction in endocytic activity, a cessation in cell division and eventually cell death. A striking morphological feature in these cells is an enlargement of the flagellar pocket, which is consistent with an imbalance in traffic to and from the surface. In contrast TbMyo1 is distributed throughout procyclic forms of the tsetse vector and a loss of approximately 90% of the protein has no obvious effects on growth or morphology. These results reveal a life cycle stage specific requirement for this myosin in essential endocytic traffic and represent the first description of the involvement of a motor protein in vesicle traffic in these parasites.
authors.affiliation: Molecular Parasitology Group, School of Biochemistry and Immunology, Trinity College Dublin, Dublin, Ireland.
authors.forename: Diana
authors.initials: D
authors.lastname: Spitznagel
authors.forename: John F
authors.initials: JF
authors.lastname: O'Rourke
authors.forename: Neal
authors.initials: N
authors.lastname: Leddy
authors.forename: Orla
authors.initials: O
authors.lastname: Hanrahan
authors.forename: Derek P
authors.initials: DP
authors.lastname: Nolan
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D005786:Gene Expression Regulation; D008969:Molecular Sequence Data; D024461:Myosin Type I / Q000097:blood / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D021381:Protein Transport; D051381:Rats; D022161:Transport Vesicles / Q000378:metabolism*; D014346:Trypanosoma brucei brucei / Q000166:cytology* / Q000201:enzymology* / Q000378:metabolism
chemical_list: D024461:Myosin Type I
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