Diagnosis of prosthetic joint infection by beadmill processing of a periprosthetic specimen.
We report a microbiological process for the documentation of prosthetic joint infection (PJI). Intraoperative periprosthetic tissue samples from 92 consecutive patients undergoing revision surgery for PJI were submitted to mechanized beadmill processing: specimens were aseptically collected in polypropylene vials, filled with sterile water and glass beads and submitted to mechanized agitation with a beadmill. The documentation rate of PJI following culture on solid and liquid media was 83.7% and the contamination rate 8.7%. Final documentation was obtained after overnight culture for 51.9% of cases and with 7 days of broth culture for all documented cases.
Roux
A-L
AL
Université de Versailles Saint-Quentin-en-Yvelines, EA3647, Garches, France.
Sivadon-Tardy
V
V
Bauer
T
T
Lortat-Jacob
A
A
Herrmann
J-L
JL
Gaillard
J-L
JL
Rottman
M
M
D002177:Candidiasis / Q000175:diagnosis* / Q000209:etiology; D018929:Cell Culture Techniques; D005260:Female; D016905:Gram-Negative Bacterial Infections / Q000175:diagnosis* / Q000209:etiology; D016908:Gram-Positive Bacterial Infections / Q000175:diagnosis* / Q000209:etiology; D006801:Humans; D007595:Joint Prosthesis / Q000009:adverse effects* / Q000382:microbiology; D008297:Male; D008828:Microbiological Techniques; D008875:Middle Aged; D016459:Prosthesis-Related Infections / Q000175:diagnosis* / Q000209:etiology