title: Early diagnosis of Dengue infection using a commercial Dengue Duo rapid test kit for the detection of NS1, IGM, and IGG.
abstract: A commercial Dengue Duo rapid test kit was evaluated for early dengue diagnosis by detection of dengue virus NS1 antigen and immunoglobulin M (IgM)/IgG antibodies. A total of 420 patient serum samples were subjected to real-time reverse transcription-polymerase chain reaction (RT-PCR), in-house IgM capture enzyme-linked immunosorbent assay (ELISA), hemagglutination inhibition assay, and the SD Dengue Duo rapid test. Of the 320 dengue acute and convalescent sera, dengue infection was detected by either serology or RT-PCR in 300 samples (93.75%), as compared with 289 samples (90.31%) in the combined SD Duo NS1/IgM. The NS1 detection rate is inversely proportional, whereas the IgM detection rate is directly proportional to the presence of IgG antibodies. The sensitivity and specificity in diagnosing acute dengue infection in the SD Duo NS1/IgM were 88.65% and 98.75%, respectively. The assay is sensitive and highly specific. Detection of both NS1 and IgM by SD Duo gave comparable detection rate by either serology or RT-PCR.
authors.affiliation: Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia. seokmuiwang@yahoo.com
authors.forename: Seok Mui
authors.initials: SM
authors.lastname: Wang
authors.forename: Shamala Devi
authors.initials: SD
authors.lastname: Sekaran
mesh_terms: D003715:Dengue / Q000175:diagnosis*; D042241:Early Diagnosis; D004797:Enzyme-Linked Immunosorbent Assay; D006386:Hemagglutination Tests; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D011933:Reagent Kits, Diagnostic; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011933:Reagent Kits, Diagnostic
references.citation: PLoS Negl Trop Dis. 2008 Aug 20;2(8):e280
references.pmid: 18714359
references.citation: Am J Trop Med Hyg. 1977 May;26(3):533-7
references.pmid: 326071
references.citation: J Virol Methods. 2007 Mar;140(1-2):75-9
references.pmid: 17140671
references.citation: Am J Trop Med Hyg. 1984 Jan;33(1):158-65
references.pmid: 6364855
references.citation: Adv Virus Res. 1999;53:35-70
references.pmid: 10582094
references.citation: Diagn Microbiol Infect Dis. 2008 Jan;60(1):43-9
references.pmid: 17889487
references.citation: Am J Trop Med Hyg. 1958 Sep;7(5):561-73
references.pmid: 13571577
references.citation: Clin Diagn Lab Immunol. 2004 Jul;11(4):642-50
references.pmid: 15242935
references.citation: Diagn Microbiol Infect Dis. 2009 May;64(1):31-6
references.pmid: 19232865
references.citation: JAMA. 2000 Dec 20;284(23):3043-5
references.pmid: 11122593
references.citation: Southeast Asian J Trop Med Public Health. 2001 Sep;32(3):472-5
references.pmid: 11944701
references.citation: J Clin Microbiol. 2000 Mar;38(3):1053-7
references.pmid: 10698995
references.citation: Diagn Microbiol Infect Dis. 2008 Apr;60(4):387-91
references.pmid: 18191361
references.citation: Clin Vaccine Immunol. 2006 Nov;13(11):1185-9
references.pmid: 16988003
references.citation: Singapore Med J. 2007 Jul;48(7):662-8
references.pmid: 17609830
references.citation: J Clin Microbiol. 2002 Feb;40(2):376-81
references.pmid: 11825945
references.citation: Southeast Asian J Trop Med Public Health. 1987 Dec;18(4):532-8
references.pmid: 3329413