title: MPC polymer regulates fibrous tissue formation by modulating cell adhesion to the biomaterial surface.
abstract: The aim of this study was to analyze the effects of 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer on fibrous tissue formation and cell adhesion plaque (CAP)-forming reactions. Silastic elastomer (SE) plates coated (experimental group) and uncoated (control group) with MPC polymer were prepared for in vivo and in vitro experiments. For the in vivo animal experiments, SE plates were implanted subcutaneously in the rat dorsal region. At 4, 8, and 12 weeks, thicknesses of the fibrous tissue capsules in the experimental group were lower than in the control group. Likewise, the amount of collagen in the experimental group was lower than that of the control group. For the in vitro cell culture experiments, KMST-6 fibroblast cells in the experimental group demonstrated enhanced cell migration, accompanied with a weaker expression of vinculin and a larger amount of filopodia. Furthermore, weaker expressions of paxillin, talin, and ROCK1, but stronger expression of cofilin, were observed in the experimental group. Taken together, these results suggested that MPC polymer regulated fibrous tissue formation by modulating cell adhesion through changes in local CAPs and downstream signaling.
authors.affiliation: Department of Physical Medicine and Rehabilitation, Graduate School of Biomedical Engineering, Tohoku University, 4-1 Seiryo-machi, Aoba-ku, Sendai 980-8575, Japan. zhangye@m.tains.tohoku.ac.jp
authors.forename: Ye
authors.initials: Y
authors.lastname: Zhang
authors.forename: Hiroyasu
authors.initials: H
authors.lastname: Kanetaka
authors.forename: Yuya
authors.initials: Y
authors.lastname: Sano
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Kano
authors.forename: Tada-aki
authors.initials: TA
authors.lastname: Kudo
authors.forename: Yoshinaka
authors.initials: Y
authors.lastname: Shimizu
mesh_terms: D051339:Actin Depolymerizing Factors / Q000032:analysis; D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000494:pharmacology; D003094:Collagen / Q000032:analysis; D004577:Electron Probe Microanalysis; D005347:Fibroblasts / Q000187:drug effects; D005353:Fibronectins / Q000032:analysis; D051416:Focal Adhesion Protein-Tyrosine Kinases / Q000032:analysis; D006801:Humans; D008297:Male; D008422:Materials Testing; D008689:Methacrylates / Q000494:pharmacology*; D051419:Paxillin / Q000032:analysis; D010767:Phosphorylcholine / Q000031:analogs & derivatives* / Q000494:pharmacology; D011108:Polymers / Q000494:pharmacology; D011554:Pseudopodia / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D012826:Silicone Elastomers / Q000737:chemistry; D013052:Spectrometry, X-Ray Emission; D040521:Subcutaneous Tissue / Q000187:drug effects* / Q000473:pathology; D016608:Talin / Q000032:analysis; D013997:Time Factors; D016596:Vinculin / Q000032:analysis; D054460:rho-Associated Kinases / Q000032:analysis
chemical_list: D051339:Actin Depolymerizing Factors; D001672:Biocompatible Materials; D020099:Coated Materials, Biocompatible; D005353:Fibronectins; D008689:Methacrylates; C496061:PXN protein, human; D051419:Paxillin; D011108:Polymers; D012826:Silicone Elastomers; D016608:Talin; C078082:silastic 4-4210; D010767:Phosphorylcholine; D016596:Vinculin; C070638:2-methacryloyloxyethyl phosphorylcholine; D003094:Collagen; D051416:Focal Adhesion Protein-Tyrosine Kinases; C515851:ROCK1 protein, human; D054460:rho-Associated Kinases