CYP 1A1 polymorphism and organochlorine pesticides levels in the etiology of prostate cancer.
Organochlorine pesticides (OCPs) and polymorphisms of xenobiotic metabolizing enzymes are reported to be associated with the possible risk of prostate cancer. OCPs are endocrine disruptors (EDs) which may act by disrupting the physiologic function of endogenous hormones and therefore possibly increase prostate cancer risk. CYP1A1 metabolizes several carcinogens and estrogens, etc. and hence polymorphism of this gene has been reported to be associated with prostate cancer risk. We studied 70 newly diagnosed prostate cancer patients and 61 age-matched healthy male controls. OCP levels in blood were determined by using gas chromatography-mass spectrometry (GC-MS) and CYP1A1 polymorphisms were analyzed by allele-specific PCR and RFLP-PCR methods. Significantly higher levels of β-HCH, γ-HCH and p,p'-DDE were found in cases as compared to controls (p-values=0.04, 0.008, and 0.01, respectively). Higher levels of γ-HCH were observed in advanced stages of prostate cancer cases (<or=T(2) vs. >or=T(3)), (p-value=0.04). Dieldrin was found significantly higher in cases with initial stages (p-value=0.03). We did not observe any correlation between prostate cancer and CYP1A1 polymorphisms. Hence, higher level of OCPs, especially β-HCH, γ-HCH and p,p'-DDE might be associated with prostate cancer risk.
Kumar
Vivek
V
Environmental Biochemistry & Molecular Biology Laboratory, Department of Biochemistry, University College of Medical Sciences & GTB Hospital, University of Delhi, Dilshad Garden, Delhi 110 095, India.
Yadav
Chandra Shekhar
CS
Singh
Satyender
S
Goel
Sanjay
S
Ahmed
Rafat Sultana
RS
Gupta
Sanjay
S
Grover
Rajesh Kumar
RK
Banerjee
Basu Dev
BD
D000368:Aged; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics* / Q000378:metabolism; D052244:Endocrine Disruptors / Q000097:blood / Q000633:toxicity; D004785:Environmental Pollutants / Q000097:blood / Q000633:toxicity; D020022:Genetic Predisposition to Disease; D006801:Humans; D006843:Hydrocarbons, Chlorinated / Q000097:blood / Q000633:toxicity*; D008297:Male; D008875:Middle Aged; D010575:Pesticides / Q000097:blood / Q000633:toxicity*; D020641:Polymorphism, Single Nucleotide; D011471:Prostatic Neoplasms / Q000097:blood / Q000209:etiology* / Q000235:genetics; D012307:Risk Factors
D052244:Endocrine Disruptors; D004785:Environmental Pollutants; D006843:Hydrocarbons, Chlorinated; D010575:Pesticides; D019363:Cytochrome P-450 CYP1A1