title: Variability of the polymerase gene (NS5B) in hepatitis C virus-infected women.
abstract: There are limited data on diversity within the hepatitis C virus polymerase (NS5B). In concordance with its key functional role during the life cycle, NS5B intrapatient variability was low. Moreover, differences between NS5B nonsynonymous (dN) and synonymous (dS) mutation rates (dN - dS) were positively correlated with CD4 cell count, while nonsynonymous mutations were strongly correlated with reduced replication in vivo.
authors.affiliation: Department of Internal Medicine, Division of Digestive Diseases, University of Cincinnati College of Medicine, Cincinnati, Ohio 45267, USA. jason.blackard@uc.edu
authors.forename: Jason T
authors.initials: JT
authors.lastname: Blackard
authors.forename: Gang
authors.initials: G
authors.lastname: Ma
authors.forename: Berkeley N
authors.initials: BN
authors.lastname: Limketkai
authors.forename: Jeffrey A
authors.initials: JA
authors.lastname: Welge
authors.forename: Peter D
authors.initials: PD
authors.lastname: Dryer
authors.forename: Christina M
authors.initials: CM
authors.lastname: Martin
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Hiasa
authors.forename: Lynn E
authors.initials: LE
authors.lastname: Taylor
authors.forename: Kenneth H
authors.initials: KH
authors.lastname: Mayer
authors.forename: Denise J
authors.initials: DJ
authors.lastname: Jamieson
authors.forename: Kenneth E
authors.initials: KE
authors.lastname: Sherman
mesh_terms: D000595:Amino Acid Sequence; D018791:CD4 Lymphocyte Count; D005260:Female; D014644:Genetic Variation; D016174:Hepacivirus / Q000145:classification* / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D006526:Hepatitis C / Q000276:immunology / Q000821:virology*; D006801:Humans; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D017361:Viral Nonstructural Proteins / Q000235:genetics*; D012324:RNA-Dependent RNA Polymerase
chemical_list: D017361:Viral Nonstructural Proteins; C087036:NS-5 protein, hepatitis C virus; D012324:RNA-Dependent RNA Polymerase
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