title: Prestressed nuclear organization in living cells.
abstract: The nucleus is maintained in a prestressed state within eukaryotic cells, stabilized mechanically by chromatin structure and other nuclear components on its inside, and cytoskeletal components on its outside. Nuclear architecture is emerging to be critical to the governance of chromatin assembly, regulation of genome function and cellular homeostasis. Elucidating the prestressed organization of the nucleus is thus important to understand how the nuclear architecture impinges on its function. In this chapter, various chemical and mechanical methods have been described to probe the prestressed organization of the nucleus.
authors.affiliation: National Centre for Biological Sciences, Tata Institute of Fundamental Research, Bellary Road, Bangalore 560065, India.
authors.forename: Aprotim
authors.initials: A
authors.lastname: Mazumder
authors.forename: T
authors.initials: T
authors.lastname: Roopa
authors.forename: Abhishek
authors.initials: A
authors.lastname: Kumar
authors.forename: K Venkatesan
authors.initials: KV
authors.lastname: Iyer
authors.forename: Nisha M
authors.initials: NM
authors.lastname: Ramdas
authors.forename: G V
authors.initials: GV
authors.lastname: Shivashankar
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena / Q000187:drug effects / Q000235:genetics / Q000502:physiology / Q000528:radiation effects; D002467:Cell Nucleus / Q000235:genetics* / Q000502:physiology* / Q000648:ultrastructure; D002477:Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D042002:Chromatin Assembly and Disassembly / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D003599:Cytoskeleton / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006801:Humans; D007834:Lasers; D008954:Models, Biological; D038761:Photobleaching; D013314:Stress, Mechanical
chemical_list: D004791:Enzyme Inhibitors