title: Development of an up-converting phosphor technology-based 10-channel lateral flow assay for profiling antibodies against Yersinia pestis.
abstract: In this study, a 10-channel up-converting phosphor technology-based lateral flow (TC-UPT-LF) assay was developed to profile antibodies against Yersinia pestis. Ten expressed Y. pestis proteins were covalently conjugated with an up-converting phosphor particle to develop double-antigen sandwich immunochromatographic strips to detect corresponding antibodies. After optimization one by one, each strip was integrated into a TC-UPT-LF disc for simultaneously detection of different antibodies. A scanning biosensor was also developed to acquire the results. The performance of the TC-UPT-LF assay was evaluated by using standard samples and plague monkey serum samples. Fifty-one patient serum samples were detected by the TC-UPT-LF assay. The TC-UPT-LF disc could be stable for 10 days at 37°C with an average CV of 10.3%. Its sensitivity and qualitative results are comparable to those of ELISA. Its linearity fitting coefficient of determination (R2) for different antibody detection is between 0.93 and 0.99. Besides F1 antibody, the LcrV and YopD antibodies also showed higher positive ratio than the other seven antibodies, as 100% (13/13) and 92% (12/13) in monkey sera and 86.3% (44/51) and 66.7% (34/51) in patient sera, respectively. It is suggested that the TC-UPT-LF assay has been successfully developed for multi-detection and LcrV and YopD can be the potential diagnostic markers of the plague.
authors.affiliation: Laboratory of Analytical Microbiology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, No. 20, Dongdajie, Fengtai District, Beijing 100071, People's Republic of China.
authors.forename: Wenyan
authors.initials: W
authors.lastname: Hong
authors.forename: Lihua
authors.initials: L
authors.lastname: Huang
authors.forename: Haoran
authors.initials: H
authors.lastname: Wang
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Qu
authors.forename: Zhaobiao
authors.initials: Z
authors.lastname: Guo
authors.forename: Chengke
authors.initials: C
authors.lastname: Xie
authors.forename: Ziwen
authors.initials: Z
authors.lastname: Zhu
authors.forename: Youbao
authors.initials: Y
authors.lastname: Zhang
authors.forename: Zongmin
authors.initials: Z
authors.lastname: Du
authors.forename: Yanfeng
authors.initials: Y
authors.lastname: Yan
authors.forename: Yan
authors.initials: Y
authors.lastname: Zheng
authors.forename: Huijie
authors.initials: H
authors.lastname: Huang
authors.forename: Ruifu
authors.initials: R
authors.lastname: Yang
authors.forename: Lei
authors.initials: L
authors.lastname: Zhou
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis* / Q000097:blood*; D000942:Antigens, Bacterial; D015374:Biosensing Techniques / Q000379:methods*; D000882:Haplorhini; D006801:Humans; D007118:Immunoassay / Q000379:methods; D010930:Plague / Q000175:diagnosis*; D012680:Sensitivity and Specificity; D015010:Yersinia pestis / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial