Novel polyhydroxylated fullerene suppresses intracellular oxidative stress together with repression of intracellular lipid accumulation during the differentiation of OP9 preadipocytes into adipocytes.
Along with differentiation of mouse stromal preadipocytes OP9 into adipocytes, intracellular ROS, especially superoxide anion radicals detected by NBT reduction assay, were found to appreciably increase, mainly in cytoplasmic area, parallelling with increases in intracellular lipid-droplet accumulation, whereas undifferentiated OP9 cells kept lower levels of ROS and lipid-droplets. beta-Carotene bleaching assay showed that super-highly hydroxylated fullerene (SHH-F; C(60) (OH)(44)) exerted higher antioxidant ability than highly hydroxylated fullerene (HH-F; C(60) (OH)(32-34)) or lowly hydroxylated fullerene (LH-F; C(60) (OH)(6-12)). Differentiation-dependent lipid-droplet accumulation was suppressed by SHH-F or HH-F more efficiently than LH-F. Furthermore, SHH-F significantly repressed intracellular ROS generation accompanied by adipocyte differentiation. Thus, lipid-droplet accumulation was shown to positively correlate with ROS upon the differentiation of OP9 preadipocytes into adipocytes and SHH-F significantly suppressed intracellular ROS together with repression of intracellular lipid accumulation.
Saitoh
Yasukazu
Y
Prefectural University of Hiroshima, Shobara, Hiroshima, Japan.
Xiao
Li
L
Mizuno
Hiromi
H
Kato
Shinya
S
Aoshima
Hisae
H
Taira
Hikaru
H
Kokubo
Ken
K
Miwa
Nobuhiko
N
D017667:Adipocytes / Q000187:drug effects* / Q000378:metabolism; D050156:Adipogenesis / Q000187:drug effects; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D015536:Down-Regulation / Q000187:drug effects; D004353:Drug Evaluation, Preclinical; D037741:Fullerenes / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D017665:Hydroxyl Radical / Q000737:chemistry / Q000378:metabolism; D042541:Intracellular Space / Q000187:drug effects / Q000378:metabolism; D050356:Lipid Metabolism / Q000187:drug effects*; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism; D013997:Time Factors
D037741:Fullerenes; D017382:Reactive Oxygen Species; D013481:Superoxides; D017665:Hydroxyl Radical