Copper tolerance and response of antioxidative enzymes in axenically grown Brassica juncea (L.) plants.
Copper is an essential element for proper functioning of all living organisms including plants, but it can cause toxicity at elevated concentrations. In the present study, two varieties of Brassica juncea L. i.e. Pusa JK and TM 4 grown axenically were compared for Cu tolerance and accumulation ability. For further detailed biochemical studies, var. TM 4 was used because of its fast growth and better Cu accumulation in shoots. Toxic effects of Cu were manifested by a reduction in photosynthetic pigments and an increase in the levels of thiobarbituric acid reactive substances. The activities of antioxidant enzymes such as superoxide dismutase, ascorbate peroxidase, guaiacol peroxidase and catalase showed an increase in a concentration and exposure time dependent manner in roots of B. juncea exposed to copper, indicating that they play an important role in combating copper stress in this species.
Singh
Sudhir
S
Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Mumbai 400085, India.
Singh
Shraddha
S
Ramachandran
V
V
Eapen
Susan
S
D000975:Antioxidants / Q000378:metabolism*; D060387:Ascorbate Peroxidases; D002374:Catalase / Q000378:metabolism; D003300:Copper / Q000633:toxicity*; D004798:Enzymes / Q000378:metabolism*; D015227:Lipid Peroxidation / Q000187:drug effects; D009149:Mustard Plant / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D009195:Peroxidase / Q000378:metabolism; D010544:Peroxidases / Q000378:metabolism; D010788:Photosynthesis; D010860:Pigments, Biological / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D018520:Plant Shoots / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D012989:Soil Pollutants / Q000737:chemistry / Q000633:toxicity*; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D013997:Time Factors
D000975:Antioxidants; D004798:Enzymes; D010860:Pigments, Biological; D012989:Soil Pollutants; D017392:Thiobarbituric Acid Reactive Substances; D003300:Copper; D010544:Peroxidases; C121048:guaiacol peroxidase; D060387:Ascorbate Peroxidases; D002374:Catalase; D009195:Peroxidase; D013482:Superoxide Dismutase