Crystallization and preliminary X-ray crystallographic analysis of a bacterial tyrosinase from Bacillus megaterium.
Tyrosinases are type 3 copper enzymes that are involved in the production of melanin and have two copper ions in the active site. Here, the crystallization and primary analysis of a tyrosinase from Bacillus megaterium is reported. The purified protein was crystallized in the absence or presence of zinc ions and the crystals diffracted to a resolution of 2.0 A. Crystals obtained in the presence of zinc belonged to space group P2(1)2(1)2(1), while crystals grown in the absence of zinc belonged to space group P2(1). In both space groups the asymmetric unit contained a dimer, with minor differences in the crystal density and in packing interactions.
Sendovski
Mor
M
Department of Biotechnology and Food Engineering, Technion-Israel Institute of Technology, Haifa 32000, Israel.
Kanteev
Margarita
M
Shuster Ben-Yosef
Vered
V
Adir
Noam
N
Fishman
Ayelet
A
D001410:Bacillus megaterium / Q000201:enzymology*; D003460:Crystallization; D018360:Crystallography, X-Ray; D008958:Models, Molecular; D014442:Monophenol Monooxygenase / Q000737:chemistry*; D017434:Protein Structure, Tertiary
D014442:Monophenol Monooxygenase
Acc Chem Res. 2002 Mar;35(3):183-91
11900522
Acta Crystallogr D Biol Crystallogr. 1997 May 1;53(Pt 3):240-55
15299926
Protein Sci. 1993 Apr;2(4):597-619
8518732
Lett Appl Microbiol. 2004;39(5):407-12
15482430
J Agric Food Chem. 2010 Jan 27;58(2):1189-201
20039636
FEBS J. 2006 Jan;273(2):257-70
16403014
Gene. 2007 Aug 15;398(1-2):183-91
17566671
J Mol Biol. 1968 Apr 28;33(2):491-7
5700707
Nat Struct Biol. 1998 Dec;5(12):1084-90
9846879
Biotechnol Appl Biochem. 2000 Apr;31(2):113-8
10744956
Syst Appl Microbiol. 2006 Jan;29(1):3-14
16423650
Curr Opin Struct Biol. 2006 Dec;16(6):729-35
17011183
J Mol Microbiol Biotechnol. 2009;17(4):188-200
19672047
Angew Chem Int Ed Engl. 2006 Jul 10;45(28):4546-50
16795103
J Mol Biol. 1998 May 15;278(4):855-70
9614947
Proc Natl Acad Sci U S A. 2009 Oct 6;106(40):17002-6
19805072
Acta Crystallogr D Biol Crystallogr. 2004 Dec;60(Pt 12 Pt 1):2196-201
15572772
Acta Crystallogr D Biol Crystallogr. 2007 Jan;63(Pt 1):32-41
17164524
Trends Biotechnol. 2003 Dec;21(12):543-9
14624863
J Biol Chem. 2006 Mar 31;281(13):8981-90
16436386
Acc Chem Res. 2007 Jul;40(7):592-600
17461541