Vascular tone and neurovascular coupling: considerations toward an improved in vitro model.
Neurovascular research has made significant strides toward understanding how the brain neurovascular unit accomplishes rapid and spatial increases in blood flow following neuronal activation. Among the experimental models used, the in vitro brain slice preparation provides unique information revealing the potential signals and cellular mechanisms involved in functional hyperemia. The most crucial limitation of this model, however, is the lack of intraluminal pressure and flow in the vessels being studied. Moreover, differences in basal vascular tone have led to varied interpretations regarding the polarity of vascular responses following neuron-to-glial stimulation. Given the complexity of astrocyte-induced neurovascular responses, we propose the use of a modified in vitro brain slice preparation, where intraluminal arteriolar pressure and flow are retained. Throughout this review, we discuss the advantages and disadvantages to be considered when using brain slices for neurovascular studies. Potential ways to overcome the current limitations are proposed.
Filosa
Jessica A
JA
Department of Physiology, Medical College of Georgia Augusta, GA, USA.
Nat Neurosci. 1998 Aug;1(4):263-5
10195155
Glia. 1998 May;23(1):1-10
9562180
Am J Physiol Heart Circ Physiol. 2004 Jul;287(1):H331-9
14962837
Nat Rev Neurosci. 2010 Feb;11(2):87-99
20087359
Neurosci Res. 2007 Oct;59(2):160-71
17655958
J Physiol. 2004 Aug 15;559(Pt 1):3-15
15218071
Glia. 1999 Jun;26(4):280-90
10383047
J Neurosci. 2006 Jun 28;26(26):6997-7006
16807329
Clin Exp Pharmacol Physiol. 2008 Sep;35(9):1116-20
18215190
J Neurosci Methods. 1995 Jun;59(1):5-9
7475251
J Neurochem. 2000 Mar;74(3):1263-70
10693960
Stroke. 2009 Apr;40(4):1451-7
19246694
J Neurophysiol. 2007 Aug;98(2):1030-41
17553943
Prog Brain Res. 2004;145:171-8
14650915
Brain Res Rev. 2010 May;63(1-2):138-48
19948187
In Vivo. 2004 May-Jun;18(3):357-66
15341192
Am J Physiol Heart Circ Physiol. 2008 Feb;294(2):H622-32
18055520
Brain Res. 1995 Oct 2;694(1-2):78-84
8974667
Am J Physiol Renal Physiol. 2002 Jun;282(6):F1097-102
11997326
J Neurosci Methods. 2009 Oct 15;183(2):107-13
19524611
J Neurochem. 2004 Dec;91(5):1171-9
15569260
J Appl Physiol (1985). 2006 Jan;100(1):318-27
16357085
J Neurosci. 2003 Oct 8;23(27):9254-62
14534260
Circ Res. 1995 Oct;77(4):832-40
7554130
News Physiol Sci. 2002 Feb;17:27-31
11821533
J Neurophysiol. 2008 May;99(5):2035-47
17913979
Clin Sci (Lond). 2009 Jul 16;117(4):139-55
19601928
J Appl Physiol (1985). 2006 Mar;100(3):1059-64
16467392
Nature. 2004 Sep 9;431(7005):137-8
15356612
Neuroscience. 1996 Oct;74(3):927-34
8884787
Br J Pharmacol. 2009 Feb;156(4):545-62
19187341
J Neurosci. 2004 Oct 13;24(41):8940-9
15483113
J Intern Med. 2010 Feb;267(2):156-71
20175864
Science. 1990 Jan 26;247(4941):470-3
1967852
J Physiol. 2003 Dec 1;553(Pt 2):407-14
14500777
Proc Natl Acad Sci U S A. 2006 Jun 27;103(26):10058-63
16782808
Acta Biochim Pol. 2009;56(3):393-404
19753330
Nat Neurosci. 2006 Feb;9(2):260-7
16388306
Hypertension. 1997 Jan;29(1 Pt 2):320-5
9039122
Free Radic Biol Med. 2010 Apr 15;48(8):1044-50
20100565
Acta Neurol Belg. 2004 Jun;104(2):51-6
15508267
Biophys J. 1991 Nov;60(5):1229-42
1760509
Arterioscler Thromb Vasc Biol. 2010 Apr;30(4):851-8
20093626
Methods Mol Med. 2003;86:413-27
12886785
Expert Opin Ther Targets. 2004 Aug;8(4):321-34
15268627
Science. 1995 Oct 27;270(5236):633-7
7570021
Nature. 2004 Sep 9;431(7005):195-9
15356633
Antioxid Redox Signal. 2009 Feb;11(2):251-66
18831678
Neuroscience. 1997 Aug;79(3):827-36
9219945
Hypertension. 2009 Mar;53(3):532-8
19188524
J Neurosci. 2003 Nov 12;23(32):10302-10
14614089
Am J Physiol. 1984 Mar;246(3 Pt 2):H380-5
6703074
J Neurosci. 1995 Nov;15(11):7427-41
7472495
Nat Neurosci. 1998 Aug;1(4):286-9
10195161
Curr Hypertens Rep. 2007 Jun;9(3):242-7
17519132
J Neurosci. 2003 Mar 1;23(5):1678-87
12629172
Front Biosci. 2008 May 01;13:4198-217
18508506
Nature. 2008 Dec 11;456(7223):745-9
18971930
J Neurosci. 2007 Apr 18;27(16):4452-9
17442830
Front Hum Neurosci. 2009 Feb 24;3:2
19287480
Circ J. 2010 Apr;74(4):608-16
20234102
Nat Neurosci. 2006 Nov;9(11):1397-1403
17013381
J Cereb Blood Flow Metab. 2010 Apr;30(4):757-68
20040927
Exp Physiol. 2005 Jan;90(1):131-40
15466455
Am J Physiol. 1999 Aug;277(2):H799-811
10444508
J Physiol. 1998 Apr 1;508 ( Pt 1):199-209
9490839
Am J Physiol Heart Circ Physiol. 2003 Nov;285(5):H2054-63
12881223
Am J Physiol. 1984 Mar;246(3 Pt 2):F349-58
6703068
Brain Lang. 2007 Aug;102(2):141-52
17010421
Trends Neurosci. 2009 Mar;32(3):160-9
19162338
J Cardiovasc Pharmacol. 2004 Jul;44(1):1-8
15175551
Front Syst Neurosci. 2009 Sep 08;3:9
19826613
J Neurosci. 1994 Aug;14(8):5068-76
8046468
Trends Neurosci. 2005 Apr;28(4):188-95
15808353
Proc Natl Acad Sci U S A. 2010 Feb 23;107(8):3811-6
20133576
Peptides. 2001 Jun;22(6):995-1007
11390030
Am J Physiol. 1999 Dec;277(6):R1760-70
10600924
Nat Neurosci. 2003 Jan;6(1):43-50
12469126
Nat Neurosci. 2007 Nov;10(11):1369-76
17965657
J Neurosci. 2000 Sep 15;20(18):6968-73
10995841
J Neurochem. 2001 Mar;76(5):1404-10
11238725
J Neurochem. 2004 Jan;88(1):246-56
14675168
J Neurosci. 2002 Nov 1;22(21):9430-44
12417668
Circ Res. 2004 Nov 12;95(10):e73-81
15499024
Proc Natl Acad Sci U S A. 2005 Nov 29;102(48):17483-8
16293699
Glia. 2002 Dec;40(3):312-23
12420311
Neurosci Lett. 2000 Nov 10;294(1):9-12
11044574
Eur J Neurosci. 2000 Aug;12(8):2769-80
10971619
Am J Physiol. 1982 Oct;243(4):H598-606
7124967
J Neurosci. 2006 Mar 15;26(11):2862-70
16540563
Prog Neurobiol. 1988;31(1):1-18
3287453
Am J Physiol Heart Circ Physiol. 2008 Jun;294(6):H2855-63
18456724
Neuroscience. 1999;92(1):47-60
10392829