The role of Pvs28 in sporozoite development in Anopheles sinensis and its longevity in BALB/c mice.
To develop a vivax malaria vaccine for blocking malarial transmission, the ookinete surface protein Pvs28 was cloned from Korean malaria patients using polymerase chain reaction. The Pvs28 gene consists of 726bp and encodes 241 amino acids. It was subcloned into the expression vector pQE30 and expressed in Escherichia coli. The expressed recombinant protein, rPvs28, has a molecular weight of about 28 kDa in SDS-PAGE analysis. A monoclonal antibody against rPvs28 was produced using BALB/c mice. It inhibited sporozoite development in Anopheles sinensis mosquitoes (n = 81) which is one of the malaria vectors in Korea, with relatively high antibody titer against rPv28 persisting for more than 6 months. These results indicate that rPvs28 induces an immune response in mice that effectively blocks sporozoite development in mosquitoes. Therefore it could be a vaccine candidate for preventing vivax malaria in Korea.
Kim
Tong-Soo
TS
Department of Parasitology, College of Medicine, Inha University, Incheon 405-751, Republic of Korea.
Kim
Hyung-Hwan
HH
Moon
Sung-Ung
SU
Lee
Sun-Sim
SS
Shin
E-Hyun
EH
Oh
Chang-Mi
CM
Kang
Yoon-Joong
YJ
Kim
Do-Kyung
DK
Sohn
Youngjoo
Y
Kim
Hyuck
H
Lee
Hyeong-Woo
HW
D000818:Animals; D000852:Anopheles / Q000469:parasitology*; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology; D000913:Antibodies, Protozoan / Q000096:biosynthesis / Q000097:blood; D000953:Antigens, Protozoan / Q000235:genetics / Q000276:immunology*; D000954:Antigens, Surface / Q000235:genetics / Q000276:immunology*; D015153:Blotting, Western; D003001:Cloning, Molecular; D016207:Cytokines / Q000096:biosynthesis; D016054:DNA, Protozoan / Q000737:chemistry; D005260:Female; D006801:Humans; D007303:Insect Vectors / Q000469:parasitology*; D017780:Malaria Vaccines / Q000235:genetics / Q000276:immunology*; D016780:Malaria, Vivax / Q000517:prevention & control / Q000635:transmission; D051379:Mice; D008807:Mice, Inbred BALB C; D010966:Plasmodium vivax / Q000235:genetics / Q000254:growth & development / Q000276:immunology*; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology
D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D000954:Antigens, Surface; D016207:Cytokines; D016054:DNA, Protozoan; D017780:Malaria Vaccines; C121474:Pvs28 protein, P vivax; D011994:Recombinant Proteins