title: The role of Pvs28 in sporozoite development in Anopheles sinensis and its longevity in BALB/c mice.
abstract: To develop a vivax malaria vaccine for blocking malarial transmission, the ookinete surface protein Pvs28 was cloned from Korean malaria patients using polymerase chain reaction. The Pvs28 gene consists of 726bp and encodes 241 amino acids. It was subcloned into the expression vector pQE30 and expressed in Escherichia coli. The expressed recombinant protein, rPvs28, has a molecular weight of about 28 kDa in SDS-PAGE analysis. A monoclonal antibody against rPvs28 was produced using BALB/c mice. It inhibited sporozoite development in Anopheles sinensis mosquitoes (n = 81) which is one of the malaria vectors in Korea, with relatively high antibody titer against rPv28 persisting for more than 6 months. These results indicate that rPvs28 induces an immune response in mice that effectively blocks sporozoite development in mosquitoes. Therefore it could be a vaccine candidate for preventing vivax malaria in Korea.
authors.affiliation: Department of Parasitology, College of Medicine, Inha University, Incheon 405-751, Republic of Korea.
authors.forename: Tong-Soo
authors.initials: TS
authors.lastname: Kim
authors.forename: Hyung-Hwan
authors.initials: HH
authors.lastname: Kim
authors.forename: Sung-Ung
authors.initials: SU
authors.lastname: Moon
authors.forename: Sun-Sim
authors.initials: SS
authors.lastname: Lee
authors.forename: E-Hyun
authors.initials: EH
authors.lastname: Shin
authors.forename: Chang-Mi
authors.initials: CM
authors.lastname: Oh
authors.forename: Yoon-Joong
authors.initials: YJ
authors.lastname: Kang
authors.forename: Do-Kyung
authors.initials: DK
authors.lastname: Kim
authors.forename: Youngjoo
authors.initials: Y
authors.lastname: Sohn
authors.forename: Hyuck
authors.initials: H
authors.lastname: Kim
authors.forename: Hyeong-Woo
authors.initials: HW
authors.lastname: Lee
mesh_terms: D000818:Animals; D000852:Anopheles / Q000469:parasitology*; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology; D000913:Antibodies, Protozoan / Q000096:biosynthesis / Q000097:blood; D000953:Antigens, Protozoan / Q000235:genetics / Q000276:immunology*; D000954:Antigens, Surface / Q000235:genetics / Q000276:immunology*; D015153:Blotting, Western; D003001:Cloning, Molecular; D016207:Cytokines / Q000096:biosynthesis; D016054:DNA, Protozoan / Q000737:chemistry; D005260:Female; D006801:Humans; D007303:Insect Vectors / Q000469:parasitology*; D017780:Malaria Vaccines / Q000235:genetics / Q000276:immunology*; D016780:Malaria, Vivax / Q000517:prevention & control / Q000635:transmission; D051379:Mice; D008807:Mice, Inbred BALB C; D010966:Plasmodium vivax / Q000235:genetics / Q000254:growth & development / Q000276:immunology*; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D000954:Antigens, Surface; D016207:Cytokines; D016054:DNA, Protozoan; D017780:Malaria Vaccines; C121474:Pvs28 protein, P vivax; D011994:Recombinant Proteins