Activity assays for extracellular signal-regulated kinase 5.
Extracellular signal-regulated kinase 5 (ERK5) is also known as big MAPK (BMK1) or MAPK7. ERK5 is 115 kDa in mass and therefore larger than the other MAPKs such as ERK1/2, JNK, and p38. Like other MAPKs, ERK5 is ubiquitously expressed in mammalian cells and is part of a three kinase cascade involving a MAPK kinase (MEK5) and MAPK kinase kinase (primarily MEKK2 and MEKK3). ERK5 is important for proliferative responses to growth factors like epidermal growth factor and stress responses such as hyperosmolarity. Upon stimulation, ERK5 rapidly translocates to the nucleus for the control of transcription. ERK5 is also critical for maintenance of vascular integrity and endothelial cell survival. In this chapter, we define methods used to measure the activation of ERK5 using different biochemical and cell-based assays.
Nakamura
Kazuhiro
K
Department of Pharmacology and Lineberger Comprehensive Cancer Center, School of Medicine, University of North Carolina, Chapel Hill, NC, USA.
Johnson
Gary L
GL
D021581:Active Transport, Cell Nucleus; D000818:Animals; D000906:Antibodies / Q000276:immunology; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D042783:Endothelial Cells / Q000378:metabolism; D057075:Enzyme Assays / Q000379:methods*; D006801:Humans; D008156:Luciferases / Q000235:genetics; D026161:MADS Domain Proteins / Q000378:metabolism; D048730:MAP Kinase Kinase Kinase 2 / Q000378:metabolism; D048748:MAP Kinase Kinase Kinase 3 / Q000378:metabolism; D064326:MEF2 Transcription Factors; D051379:Mice; D048054:Mitogen-Activated Protein Kinase 7 / Q000276:immunology / Q000378:metabolism*; D004676:Myelin Basic Protein / Q000378:metabolism; D018006:Myogenic Regulatory Factors / Q000378:metabolism; D010750:Phosphoproteins / Q000276:immunology
D000906:Antibodies; D026161:MADS Domain Proteins; D064326:MEF2 Transcription Factors; C578040:MEF2C protein, human; D004676:Myelin Basic Protein; D018006:Myogenic Regulatory Factors; D010750:Phosphoproteins; D008156:Luciferases; D048054:Mitogen-Activated Protein Kinase 7; D048730:MAP Kinase Kinase Kinase 2; D048748:MAP Kinase Kinase Kinase 3