title: Micronucleus frequency in sheep lymphocytes after in vitro exposure to fungicide tolylfluanid.
abstract: The fungicide tolylfluanid (N -dichlorofluoromethylthio-N', N -dimethyl -N -p -tolylsulfamide), was investigated by cytokinesis-block micronucleus assay. Tolylfluanid at the lowest concentration (1 x 10(- 6)mol L(- 1))did not influence significantly the frequency of micronuclei in sheep lymphocyte cultures in comparison with control (32.33 +/- 3.51/1000 binucleated cells versus 30.33 +/- 2.82/1000 binucleated cells in dimethylsulfoxide (DMSO) control, P = 0.44). Higher tolylfluanid concentrations (1 x 10(- 4) and, 1 x 10(- 5) mol L(- 1)) resulted in a significant dose-dependent increase in the number of micronuclei in comparison with control (74.00 +/- 13.00/1000 binucleated cells and 52.67 +/- 10.12/1000 binucleated cells versus 30.33 +/- 2. 82/1000 binucleated cells in DMSO control, P = 0.005 and 0.02, respectively, ANOVA followed by Tukey test P < 0.05). Many of the treated cells also possessed multiple micronuclei. Tolylfluanid did not affect the nuclear division index at all treatment concentrations. Our in vitro results thus demonstrate that tolylfluanid had a significant genotoxic effect at only the highest concentration tested.
authors.affiliation: Department of Biology, University of Presov, Presov, Slovakia. sutiakova@gmail.com
authors.forename: Irena
authors.initials: I
authors.lastname: Sutiaková
authors.forename: Natália
authors.initials: N
authors.lastname: Kovalkovicová
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Legáth
authors.forename: Václav
authors.initials: V
authors.lastname: Sutiak
mesh_terms: D000814:Aniline Compounds / Q000633:toxicity*; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D005260:Female; D005659:Fungicides, Industrial / Q000633:toxicity*; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects*; D048629:Micronuclei, Chromosome-Defective / Q000187:drug effects*; D015162:Micronucleus Tests; D009153:Mutagens / Q000633:toxicity*; D012756:Sheep; D013449:Sulfonamides / Q000633:toxicity*; D014052:Toluidines
chemical_list: D000814:Aniline Compounds; D005659:Fungicides, Industrial; D009153:Mutagens; D013449:Sulfonamides; D014052:Toluidines; C070021:N-dichlorofluoromethylthio-N',N'-dimethyl-N-p-tolylsulfamide