The TCT motif, a key component of an RNA polymerase II transcription system for the translational machinery.
The TCT motif (polypyrimidine initiator) encompasses the transcription start site of nearly all ribosomal protein genes in Drosophila and mammals. The TCT motif is required for transcription of ribosomal protein gene promoters. The TCT element resembles the Inr (initiator), but is not recognized by TFIID and cannot function in lieu of an Inr. However, a single T-to-A substitution converts the TCT element into a functionally active Inr. Thus, the TCT motif is a novel transcriptional element that is distinct from the Inr. These findings reveal a specialized TCT-based transcription system that is directed toward the synthesis of ribosomal proteins.
Parry
Trevor J
TJ
Section of Molecular Biology, University of California at San Diego, La Jolla, California 92093, USA.
Theisen
Joshua W M
JW
Hsu
Jer-Yuan
JY
Wang
Yuan-Liang
YL
Corcoran
David L
DL
Eustice
Moriah
M
Ohler
Uwe
U
Kadonaga
James T
JT
D001483:Base Sequence; D008969:Molecular Sequence Data; D012319:RNA Polymerase II / Q000378:metabolism*; D012269:Ribosomal Proteins / Q000096:biosynthesis / Q000235:genetics*; D016385:TATA Box; D035362:Transcription Factor TFIID / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000737:chemistry / Q000378:metabolism; D024363:Transcription Initiation Site; D014158:Transcription, Genetic
D012269:Ribosomal Proteins; D035362:Transcription Factor TFIID; D014157:Transcription Factors; D012319:RNA Polymerase II
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