Sesame oil accelerates kidney healing following gentamicin-induced kidney injury in rats.
OBJECTIVE
We investigated the therapeutic effect of a single dose of sesame oil against gentamicin-induced renal damage in rats.


METHODS
Experimental rats were subcutaneously injected with gentamicin (100 mg/kg/day for 7 days) to induce renal injury. Sesame oil (1, 2 or 4 ml/kg) was given orally 24 h after the last dose of gentamicin. Control rats were treated with saline only. Renal injury, histopathological examination, histochemical staining, osteopontin expression, superoxide anion, nitric oxide, peroxynitrite radical and lipid peroxidation were assessed 24 h after sesame oil administration.


RESULTS
Serum blood urea nitrogen and creatinine as well as renal osteopontin expression, superoxide anion, nitric oxide, peroxynitrite radical and lipid peroxidation levels were higher in gentamicin-treated rats than in control rats. Sesame oil significantly decreased all the tested parameters compared with gentamicin-alone rats. Furthermore, histopathological and histochemical staining showed that renal tubules had recovered and regenerated in the sesame oil-treated rats.


CONCLUSIONS
We hypothesize that a single dose of sesame oil inhibits oxidative stress to shorten the recovery period and allow the regeneration of renal tubules after the onset of gentamicin-induced renal injury in rats.
Periasamy
Srinivasan
S
Department of Environmental and Occupational Health, National Cheng Kung University College of Medicine, Tainan, Taiwan, ROC.
Liu
Chuan-Teng
CT
Hsu
Dur-Zong
DZ
Liu
Ming-Yie
MY
D000818:Animals; D001806:Blood Urea Nitrogen; D049109:Cell Proliferation / Q000187:drug effects; D003404:Creatinine / Q000097:blood; D005839:Gentamicins; D017665:Hydroxyl Radical / Q000378:metabolism; D007668:Kidney / Q000378:metabolism* / Q000473:pathology*; D007674:Kidney Diseases / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D009573:Nitrites / Q000378:metabolism; D053495:Osteopontin / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D012715:Sesame Oil / Q000494:pharmacology / Q000627:therapeutic use*; D013481:Superoxides / Q000378:metabolism
D005839:Gentamicins; D009573:Nitrites; D053495:Osteopontin; D013481:Superoxides; D017665:Hydroxyl Radical; D012715:Sesame Oil; D003404:Creatinine