Hippocampal neurons respond uniquely to topographies of various sizes and shapes.
A number of studies have investigated the behavior of neurons on microfabricated topography for the purpose of developing interfaces for use in neural engineering applications. However, there have been few studies simultaneously exploring the effects of topographies having various feature sizes and shapes on axon growth and polarization in the first 24 h. Accordingly, here we investigated the effects of arrays of lines (ridge grooves) and holes of microscale (approximately 2 microm) and nanoscale (approximately 300 nm) dimensions, patterned in quartz (SiO2), on the (1) adhesion, (2) axon establishment (polarization), (3) axon length, (4) axon alignment and (5) cell morphology of rat embryonic hippocampal neurons, to study the response of the neurons to feature dimension and geometry. Neurons were analyzed using optical and scanning electron microscopy. The topographies were found to have a negligible effect on cell attachment but to cause a marked increase in axon polarization, occurring more frequently on sub-microscale features than on microscale features. Neurons were observed to form longer axons on lines than on holes and smooth surfaces; axons were either aligned parallel or perpendicular to the line features. An analysis of cell morphology indicated that the surface features impacted the morphologies of the soma, axon and growth cone. The results suggest that incorporating microscale and sub-microscale topographies on biomaterial surfaces may enhance the biomaterials' ability to modulate nerve development and regeneration.
Fozdar
David Y
DY
Department of Mechanical Engineering, The University of Texas at Austin, 1 University Station, C2200, Austin, TX 78712, USA.
Lee
Jae Young
JY
Schmidt
Christine E
CE
Chen
Shaochen
S
D000818:Animals; D001369:Axons / Q000502:physiology; D002448:Cell Adhesion; D048430:Cell Shape; D002478:Cells, Cultured; D006624:Hippocampus / Q000166:cytology*; D008853:Microscopy; D008855:Microscopy, Electron, Scanning; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D051381:Rats; D013499:Surface Properties; D023822:Tissue Engineering / Q000295:instrumentation* / Q000379:methods*
J Cell Sci. 1997 Dec;110 ( Pt 23):2915-24
9359874
Nature. 1986 Oct 23-29;323(6090):712-5
3773996
J R Soc Interface. 2007 Apr 22;4(13):223-33
17251152
Biomaterials. 2006 Jan;27(3):460-72
16115675
Biomaterials. 2007 Jan;28(2):271-84
16919328
Nanomedicine (Lond). 2008 Apr;3(2):183-99
18373425
J Hematother Stem Cell Res. 2003 Dec;12(6):689-99
14977478
Biomaterials. 2004 Nov;25(26):5781-8
15147824
Nat Rev Mol Cell Biol. 2000 Nov;1(2):110-9
11253363
Development. 1993 Jan;117(1):401-8
8223260
Tissue Eng Part B Rev. 2008 Mar;14(1):33-51
18454633
Ann Biomed Eng. 2006 Jan;34(1):89-101
16525765
J Biomed Mater Res A. 2010 Mar 15;92(4):1398-406
19353562
J Cell Sci. 2003 May 15;116(Pt 10):1881-92
12692189
J Cell Sci. 1997 Dec;110 ( Pt 23):2905-13
9359873
Biomaterials. 2006 Mar;27(8):1251-8
16143385
Biomaterials. 1997 Dec;18(24):1573-83
9613804
Med Eng Phys. 2005 Nov;27(9):730-42
15921949
Biomaterials. 2006 Feb;27(6):842-6
16098578
Exp Cell Res. 2003 Apr 1;284(2):274-82
12651159
Nanotechnology. 2005 Oct;16(10):2420-6
20818029
Development. 1991 Jun;112(2):581-90
1794326
J Neurosci. 1988 Apr;8(4):1454-68
3282038