[Cloning and expression of prenyl transferase gene of Metarhizium ansopliae].
OBJECTIVE
To clone and identify prenyl transferase gene from Metarhizium anisopliae and to understand the gene structure and expression.


METHODS
Using Switching Mechanism At 5' end of RNA Transcript (SMART) method, we isolated the full length cDNA sequence and DNA sequence. Then we used quantitative RT-PCR analysis of the gene expression levels at different stages of colonization of host hemolymph by M. anisopliae.


RESULTS
The Mpt gene had two exons and one intron and the CDS was 1026 bp which encoded a protein with 341 amino acid residues; qRT-PCR analysis showed that the gene expression levels were significantly different, especially highly up-regulated at the late stages.


CONCLUSIONS
The Mpt gene was successfully cloned from M. anisopliae for the first time and the gene had the characteristic of high expression levels at the late stages.
Yin
Congsong
C
College of Bioengineering of Chongqing University, Genetic Engineering Research Centre of Chongqing University, Key Laboratory of Gene Function and Regulation at Chongqing, Chongqing 400030, China. yincongsong@yahoo.com.cn
Jin
Kai
K
Xia
Yuxian
Y
D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D052981:Metarhizium / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics; D008969:Molecular Sequence Data; D009387:Neoprene / Q000378:metabolism*; D016415:Sequence Alignment; D014166:Transferases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
D005656:Fungal Proteins; C016077:prenyl; D009387:Neoprene; D014166:Transferases