Gilgamesh is required for rutabaga-independent olfactory learning in Drosophila.
Cyclic AMP signaling in Drosophila mushroom body neurons, anchored by the adenylyl cyclase encoded by the rutabaga gene, is indispensable for olfactory memory formation. From a screen for new memory mutants, we identified alleles of the gilgamesh (gish) gene, which encodes a casein kinase Iγ homolog that is preferentially expressed in the mushroom body neurons. The gish-encoded kinase participates in the physiology of these neurons underlying memory formation since the mutant memory deficit was rescued with expression of a gish cDNA in these neurons only during adulthood. A cellular memory trace, detected as increased calcium influx into the α'/β' neuron processes in response to the odor used for conditioning, was disrupted in gish mutants. Epistasis experiments indicated a lack of genetic interactions between gish and rutabaga. Therefore, gish participates in a rutabaga-independent pathway for memory formation and accounts for some of the residual learning that occurs in rutabaga mutants.
Tan
Ying
Y
Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX 77030, USA. yingt@bcm.edu
Yu
Dinghui
D
Pletting
Jennifer
J
Davis
Ronald L
RL
D000262:Adenylyl Cyclases / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D030801:Animals, Genetically Modified; D001362:Avoidance Learning / Q000502:physiology*; D001522:Behavior, Animal; D047389:Casein Kinase I / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D003214:Conditioning, Classical / Q000502:physiology; D004330:Drosophila; D029721:Drosophila Proteins / Q000172:deficiency / Q000235:genetics / Q000378:metabolism* / Q000502:physiology*; D005786:Gene Expression Regulation / Q000235:genetics; D008569:Memory Disorders / Q000235:genetics; D024521:Mushroom Bodies / Q000254:growth & development / Q000378:metabolism; D009154:Mutation / Q000235:genetics; D009812:Odorants; D012903:Smell / Q000502:physiology*
D029721:Drosophila Proteins; D047389:Casein Kinase I; C479663:gish protein, Drosophila; D000262:Adenylyl Cyclases; C479926:Rut protein, Drosophila
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