Responses of Mikania micrantha, an invasive weed to elevated CO₂: induction of β-caryophyllene synthase, changes in emission capability and allelopathic potential of β-caryophyllene.
To better understand the effect of predicted elevated levels of carbon dioxide (CO₂) on an invasive weed Mikania micrantha, we constructed a suppressive subtractive hybridization (SSH) library from the leaves of M. micrantha exposed to CO₂ at 350 and 750 ppm for 6 d, and isolated a novel gene named β-caryophyllene synthase. β-Caryophyllene synthase catalyses the conversion of farnesyl diphosphate to β-caryophyllene, a volatile sesquiterpene with allelopathic potential. Real-time PCR analysis revealed that gene expression of β-caryophyllene synthase in M. micrantha leaves was strongly induced in response to elevated CO₂. Gas chromatography-mass spectrometry (GC-MS) and gas chromatography (GC) analyses showed that emission levels of β-caryophyllene from leaves of M. micrantha increased when exposed to 750 ppm CO₂. Bioassays showed that phytotoxicity of β-caryophyllene against Raphanus sativus, Brassica campestris, Lactuca sativa, and M. micrantha was dose-dependent and varied with the receptor plants and concentrations of CO₂. β-Caryophyllene displayed higher phytotoxic effects at 750 ppm than those at 350 ppm CO₂, especially on R. sativus. These results suggest that elevated atmospheric CO₂ levels may enhance biosynthesis and phytotoxicity of allelochemicals in M. micrantha, one of the worst invasive weeds in the world, which in turn might enhance its potential allelopathic effect on neighboring native plants if released in bioactive concentrations. Further investigations are required to determine the adaptive responses of both invasive and native plants to a gradual increase of atmospheric CO₂ to 750 ppm predicted over a 100 year period.
Wang
Rui-Long
RL
State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, East Campus, Guangzhou 510006, People's Republic of China.
Staehelin
Christian
C
Peng
Shao-Lin
SL
Wang
Wen-Tian
WT
Xie
Xi-Mei
XM
Lu
Hui-Ning
HN
D019883:Alkyl and Aryl Transferases / Q000378:metabolism*; D002245:Carbon Dioxide / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D008401:Gas Chromatography-Mass Spectrometry; D035742:Mikania / Q000737:chemistry / Q000187:drug effects* / Q000502:physiology; D018515:Plant Leaves / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D057685:Plant Weeds / Q000187:drug effects* / Q000502:physiology; D000081223:Polycyclic Sesquiterpenes; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012717:Sesquiterpenes / Q000378:metabolism*; D013997:Time Factors
D000081223:Polycyclic Sesquiterpenes; D012717:Sesquiterpenes; D002245:Carbon Dioxide; C024714:caryophyllene; D019883:Alkyl and Aryl Transferases; C108877:terpene synthase
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