23Na and 2H magnetic resonance studies of osteoarthritic and osteoporotic articular cartilage.
In this study, the short component of the (23)Na T(2) (T(2f)) and the (23)Na and (2)H quadrupolar interactions (nu(Q)) were measured in bone-cartilage samples of osteoarthritic (OA) and osteoporotic (OP) patients. (23)Na nu(Q) was found to increase in osteoarthritic articular cartilage relative to controls. Similar results were found in bovine cartilage following proteoglycan (PG) depletion, a condition that prevails in osteoarthritis. (23)Na nu(Q) and 1/T(2f) for articular cartilage obtained from osteoporotic patients were significantly larger than for control and osteoarthritic cartilage. Decalcification of both human and bovine articular cartilage resulted in an increase of (23)Na nu(Q) and 1/T(2f), showing the same trend as the osteoporotic samples. Differences in the ratio of the intensity of the large (2)H splitting to that of the small one in the calcified zone were also observed. In osteoporosis, this ratio was twice as large as that obtained for both control and osteoarthritic samples. The (2)H and (23)Na results can be interpreted as due to sodium ions and water molecules filling the void created by the calcium depletion and to calcium ions being located in close association with the collagen fibers. To the best of our knowledge, this is the first study reporting differences of NMR parameters in cartilage of osteoporotic patients.
Keinan-Adamsky
Keren
K
School of Chemistry, Tel Aviv University, Tel Aviv, Israel.
Shinar
Haddasah
H
Shabat
Shay
S
Brin
Yaron S
YS
Nyska
Meir
M
Navon
Gil
G
D000818:Animals; D001704:Biopolymers / Q000032:analysis*; D002358:Cartilage, Articular / Q000737:chemistry*; D002417:Cattle; D003903:Deuterium / Q000032:analysis*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D010003:Osteoarthritis / Q000378:metabolism*; D010024:Osteoporosis / Q000378:metabolism*; D012979:Sodium Radioisotopes / Q000032:analysis*
D001704:Biopolymers; D012979:Sodium Radioisotopes; D003903:Deuterium