Fast quantification of the exhaled breath condensate of oxidative stress 8-iso-prostaglandin F2alpha using on-line solid-phase extraction coupled with liquid chromatography/electrospray ionization mass spectrometry.
A method using automated on-line solid-phase extraction (SPE) liquid chromatography-electrospray tandem mass spectrometry (LC-ESI-MS/MS) for the determination of 8-iso-PGF2alpha in human exhaled breath condensate (EBC) was developed and validated. A C18 SPE column with an affinity sorbent was used for on-line extraction. A C18 column was employed for LC separation and ESI-MS/MS was utilized for detection. 8-iso-PGF2alpha-d(4) was used as an internal standard for quantitative determination. The extraction, cleanup and analysis procedures were controlled by a fully automated six-port switch valve. Identification and quantification were based on the following transitions: m/z 353-->193 for 8-iso-PGF2alpha and m/z 357-->197 for 8-iso-PGF2alpha-d(4), respectively. Good recoveries from 98.94 to 99.86% were measured and satisfactory linear ranges for these analytical compounds were determined. Intra-day and inter-day precision showed that coefficients of variance (CV) ranged from 6.5 to 8.0% and 5.2 to 6.3%, respectively. The applicability of this newly developed method was demonstrated by analyzing human EBC samples for an evaluation of the future risk of human exposure to nanoparticles.
Wang
Chien-Jen
CJ
Division of Environmental Health and Occupational Medicine, National Health Research Institutes, Zhunan, Miaoli County, Taiwan, ROC.
Yang
Ning-Hsiang
NH
Liou
Saou-Hsing
SH
Lee
Hui-Ling
HL
D001944:Breath Tests; D002138:Calibration; D002853:Chromatography, Liquid / Q000379:methods*; D015237:Dinoprost / Q000032:analysis*; D057230:Limit of Detection; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D015237:Dinoprost