Surface-modified microprojection arrays for intradermal biomarker capture, with low non-specific protein binding.
Minimally invasive biosensors are of great interest for rapid detection of disease biomarkers for diagnostic screening at the point-of-care. Here we introduce a device which extracts disease-specific biomarkers directly from the upper dermis, without the needle and syringe or resource-intensive blood processing. Using antigen-specific antibodies raised in mice as a model system, we confirm the analytical specificity and sensitivity of the antibody capture and extraction in comparison to the conventional methods based on needle/syringe blood draw followed by processing and antigen-specific ELISAs.
Corrie
Simon R
SR
The University of Queensland, Australian Institute for Bioengineering and Nanotechnology, St Lucia, QLD 4072, Australia. s.corrie1@uq.edu.au
Fernando
Germain J P
GJ
Crichton
Michael L
ML
Brunck
Marion E G
ME
Anderson
Chris D
CD
Kendall
Mark A F
MA
D000818:Animals; D015415:Biomarkers / Q000032:analysis*; D015374:Biosensing Techniques / Q000295:instrumentation*; D001800:Blood Specimen Collection / Q000295:instrumentation*; D002197:Capillary Action; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007118:Immunoassay / Q000295:instrumentation*; D051379:Mice; D008810:Mice, Inbred C57BL; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D040081:Protein Array Analysis / Q000295:instrumentation*; D012867:Skin / Q000378:metabolism*
D015415:Biomarkers