A regulatory toolbox of MiniPromoters to drive selective expression in the brain.
The Pleiades Promoter Project integrates genomewide bioinformatics with large-scale knockin mouse production and histological examination of expression patterns to develop MiniPromoters and related tools designed to study and treat the brain by directed gene expression. Genes with brain expression patterns of interest are subjected to bioinformatic analysis to delineate candidate regulatory regions, which are then incorporated into a panel of compact human MiniPromoters to drive expression to brain regions and cell types of interest. Using single-copy, homologous-recombination "knockins" in embryonic stem cells, each MiniPromoter reporter is integrated immediately 5' of the Hprt locus in the mouse genome. MiniPromoter expression profiles are characterized in differentiation assays of the transgenic cells or in mouse brains following transgenic mouse production. Histological examination of adult brains, eyes, and spinal cords for reporter gene activity is coupled to costaining with cell-type-specific markers to define expression. The publicly available Pleiades MiniPromoter Project is a key resource to facilitate research on brain development and therapies.
Portales-Casamar
Elodie
E
Centre for Molecular Medicine and Therapeutics at the Child and Family Research Institute, University of British Columbia, Vancouver, BC, Canada V5Z4H4.
Swanson
Douglas J
DJ
Liu
Li
L
de Leeuw
Charles N
CN
Banks
Kathleen G
KG
Ho Sui
Shannan J
SJ
Fulton
Debra L
DL
Ali
Johar
J
Amirabbasi
Mahsa
M
Arenillas
David J
DJ
Babyak
Nazar
N
Black
Sonia F
SF
Bonaguro
Russell J
RJ
Brauer
Erich
E
Candido
Tara R
TR
Castellarin
Mauro
M
Chen
Jing
J
Chen
Ying
Y
Cheng
Jason C Y
JC
Chopra
Vik
V
Docking
T Roderick
TR
Dreolini
Lisa
L
D'Souza
Cletus A
CA
Flynn
Erin K
EK
Glenn
Randy
R
Hatakka
Kristi
K
Hearty
Taryn G
TG
Imanian
Behzad
B
Jiang
Steven
S
Khorasan-zadeh
Shadi
S
Komljenovic
Ivana
I
Laprise
Stéphanie
S
Liao
Nancy Y
NY
Lim
Jonathan S
JS
Lithwick
Stuart
S
Liu
Flora
F
Liu
Jun
J
Lu
Meifen
M
McConechy
Melissa
M
McLeod
Andrea J
AJ
Milisavljevic
Marko
M
Mis
Jacek
J
O'Connor
Katie
K
Palma
Betty
B
Palmquist
Diana L
DL
Schmouth
Jean-François
JF
Swanson
Magdalena I
MI
Tam
Bonny
B
Ticoll
Amy
A
Turner
Jenna L
JL
Varhol
Richard
R
Vermeulen
Jenny
J
Watkins
Russell F
RF
Wilson
Gary
G
Wong
Bibiana K Y
BK
Wong
Siaw H
SH
Wong
Tony Y T
TY
Yang
George S
GS
Ypsilanti
Athena R
AR
Jones
Steven J M
SJ
Holt
Robert A
RA
Goldowitz
Daniel
D
Wasserman
Wyeth W
WW
Simpson
Elizabeth M
EM
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002454:Cell Differentiation / Q000235:genetics; D019295:Computational Biology; D030541:Databases, Genetic; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism; D015870:Gene Expression; D020869:Gene Expression Profiling / Q000706:statistics & numerical data; D055879:Gene Knock-In Techniques; D017930:Genes, Reporter; D023281:Genomics; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid
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