title: The effects of pre-pubertal gonadectomy and binge-like ethanol exposure during adolescence on ethanol drinking in adult male and female rats.
abstract: The pubertal surge in gonadal hormones that occurs during adolescence may impact the long-term effects of early alcohol exposure and sex differences in drinking behavior in adulthood. We investigated this hypothesis by performing sham or gonadectomy surgeries in Long-Evans rats around post-natal day (P) 20. From P35-45, males and females were given saline or 3.0 g/kg ethanol using a binge-like model of exposure (8 injections total). As adults (P100), they were trained to self-administer ethanol via a sucrose-fading procedure and then given access to different unsweetened concentrations (5-20%, w/v) for 5 days/concentration. We found that during adolescence, ethanol-induced intoxication was similar in males and females that underwent sham surgery. In gonadectomized males and females, however, the level of intoxication was greater following the last injection compared to the first. During adulthood, females drank more sucrose per body weight than males and binge-like exposure to ethanol reduced sucrose consumption in both sexes. These effects were not seen in gonadectomized rats. Ethanol consumption was higher in saline-exposed females compared to males, with gonadectomy reversing this sex difference by increasing consumption in males and decreasing it in females. Exposure to ethanol during adolescence augmented ethanol consumption in both sexes, but this effect was statistically significant only in gonadectomized females. Together, these results support a role for gonadal hormones during puberty in the short- and long-term effects of ethanol on behavior and in the development of sex differences in consummatory behavior during adulthood.
authors.affiliation: Department of Psychology, University of Illinois at Urbana-Champaign 603 E. Daniel St., Champaign, IL 61820, USA.
authors.forename: Luke K
authors.initials: LK
authors.lastname: Sherrill
authors.forename: Wendy A
authors.initials: WA
authors.lastname: Koss
authors.forename: Emily S
authors.initials: ES
authors.lastname: Foreman
authors.forename: Joshua M
authors.initials: JM
authors.lastname: Gulley
mesh_terms: D000367:Age Factors; D000704:Analysis of Variance; D000818:Animals; D002492:Central Nervous System Depressants / Q000008:administration & dosage / Q000506:poisoning*; D003423:Critical Period, Psychological; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004327:Drinking Behavior / Q000187:drug effects* / Q000502:physiology; D000431:Ethanol / Q000008:administration & dosage / Q000506:poisoning*; D005260:Female; D005518:Food Preferences / Q000187:drug effects* / Q000502:physiology; D012739:Gonadal Steroid Hormones / Q000502:physiology*; D008297:Male; D011627:Puberty / Q000187:drug effects / Q000502:physiology; D051381:Rats; D020318:Rats, Long-Evans; D012737:Sex Factors; D013395:Sucrose / Q000008:administration & dosage; D013549:Sweetening Agents / Q000008:administration & dosage
chemical_list: D002492:Central Nervous System Depressants; D012739:Gonadal Steroid Hormones; D013549:Sweetening Agents; D000431:Ethanol; D013395:Sucrose
references.citation: Alcohol. 2008 Dec;42(8):649-56
references.pmid: 19038696
references.citation: Life Sci. 1985 Oct 7;37(14):1293-300
references.pmid: 4046734
references.citation: Neuroscience. 2007 Feb 9;144(3):961-8
references.pmid: 17137726
references.citation: Alcohol Clin Exp Res. 2003 Oct;27(10):1606-12
references.pmid: 14574231
references.citation: Neurotoxicol Teratol. 2007 Jan-Feb;29(1):81-95
references.pmid: 17174531
references.citation: Alcohol. 2002 Jan;26(1):23-30
references.pmid: 11958943
references.citation: Alcohol Clin Exp Res. 2002 Feb;26(2):165-72
references.pmid: 11964555
references.citation: Alcohol Clin Exp Res. 2008 Dec;32(12):2100-6
references.pmid: 18828803
references.citation: Int J Dev Neurosci. 2009 Dec;27(8):805-15
references.pmid: 19712739
references.citation: Alcohol Clin Exp Res. 2005 Oct;29(10):1796-808
references.pmid: 16269909
references.citation: Alcohol Clin Exp Res. 2008 Sep;32(9):1574-82
references.pmid: 18616665
references.citation: Alcohol Clin Exp Res. 2001 Nov;25(11):1613-20
references.pmid: 11707636
references.citation: Horm Behav. 2010 Jun;58(1):82-90
references.pmid: 19854195
references.citation: Physiol Behav. 1975 Mar;14(3):353-61
references.pmid: 1169787
references.citation: Brain Res. 1985 Oct;354(2):301-4
references.pmid: 4052820
references.citation: J Child Neurol. 1998 Nov;13(11):550-6
references.pmid: 9853648
references.citation: J Comp Neurol. 2002 Nov 11;453(2):116-30
references.pmid: 12373778
references.citation: Pharmacol Biochem Behav. 1995 Sep;52(1):217-23
references.pmid: 7501668
references.citation: Neuroscience. 2003;120(2):523-33
references.pmid: 12890521
references.citation: Am J Physiol Endocrinol Metab. 2010 Feb;298(2):E320-8
references.pmid: 19952347
references.citation: Alcohol. 1998 May;15(4):311-4
references.pmid: 9590516
references.citation: Dev Psychol. 2000 Mar;36(2):180-9
references.pmid: 10749075
references.citation: Neurotoxicol Teratol. 2007 Jul-Aug;29(4):458-65
references.pmid: 17433619
references.citation: Alcohol Clin Exp Res. 2004 Dec;28(12):1907-15
references.pmid: 15608608
references.citation: Alcohol Clin Exp Res. 2007 Nov;31(11):1846-57
references.pmid: 17850218
references.citation: J Comp Neurol. 2009 Feb 20;512(6):717-25
references.pmid: 19065620
references.citation: Alcohol. 1993 Jan-Feb;10(1):37-43
references.pmid: 8447965
references.citation: Alcohol. 2004 Oct-Nov;34(2-3):177-85
references.pmid: 15902911
references.citation: Brain Res. 2008 Jun 18;1215:40-52
references.pmid: 18474362
references.citation: J Child Psychol Psychiatry. 2007 Mar-Apr;48(3-4):224-44
references.pmid: 17355397
references.citation: Ann N Y Acad Sci. 2004 Jun;1021:110-23
references.pmid: 15251880
references.citation: Alcohol Clin Exp Res. 2007 Jul;31(7):1159-68
references.pmid: 17511750
references.citation: Alcohol. 2002 Feb;26(2):83-93
references.pmid: 12007583
references.citation: Alcohol Clin Exp Res. 2001 Sep;25(9):1301-8
references.pmid: 11584149
references.citation: Alcohol Clin Exp Res. 2002 Nov;26(11):1632-41
references.pmid: 12436051
references.citation: Pharmacol Biochem Behav. 2007 Apr;86(4):637-42
references.pmid: 17395255
references.citation: Brain Res Dev Brain Res. 1994 Aug 12;81(1):113-20
references.pmid: 7805276
references.citation: Alcohol. 2005 May;36(1):55-61
references.pmid: 16257354
references.citation: Alcohol. 2003 Aug-Oct;31(1-2):87-92
references.pmid: 14615015
references.citation: Behav Brain Funct. 2009 Jun 04;5:23
references.pmid: 19497110
references.citation: Ann N Y Acad Sci. 2004 Jun;1021:431-5
references.pmid: 15251923
references.citation: Ann N Y Acad Sci. 2004 Jun;1021:422-6
references.pmid: 15251921
references.citation: Nat Neurosci. 1999 Oct;2(10):861-3
references.pmid: 10491603
references.citation: Brain Res Dev Brain Res. 2001 Aug 23;129(2):125-33
references.pmid: 11506857
references.citation: J Stud Alcohol. 1999 Jan;60(1):78-89
references.pmid: 10096312
references.citation: Pediatrics. 2004 Sep;114(3):e300-6
references.pmid: 15342890
references.citation: Alcohol Alcohol. 2009 Nov-Dec;44(6):547-54
references.pmid: 19767625
references.citation: Am J Physiol. 1998 Mar;274(3):R718-24
references.pmid: 9530238
references.citation: Alcohol Clin Exp Res. 2000 Apr;24(4):421
references.pmid: 10798574
references.citation: Alcohol Clin Exp Res. 2005 Jul;29(7):1139-45
references.pmid: 16046868
references.citation: Brain Res. 2006 Jul 12;1099(1):73-81
references.pmid: 16781682
references.citation: Brain Cogn. 2010 Feb;72(1):46-55
references.pmid: 19913969
references.citation: Neuroimage. 2010 Jan 1;49(1):63-70
references.pmid: 19683586
references.citation: Alcohol. 2003 Aug-Oct;31(1-2):49-61
references.pmid: 14615011
references.citation: Health Psychol. 1994 Jul;13(4):326-33
references.pmid: 7957011
references.citation: Bull Narc. 1980;32(2):41-8
references.pmid: 6907025
references.citation: Alcohol Clin Exp Res. 2001 Apr;25(4):594-9
references.pmid: 11329501
references.citation: Life Sci. 2000 Jul 7;67(7):765-73
references.pmid: 10968406
references.citation: Alcohol Clin Exp Res. 2007 May;31(5):755-65
references.pmid: 17386073
references.citation: Neuroscience. 2010 Mar 10;166(1):5-14
references.pmid: 20004707
references.citation: Arch Pediatr Adolesc Med. 1994 Aug;148(8):789-95
references.pmid: 8044254
references.citation: Synapse. 2000 Aug;37(2):167-9
references.pmid: 10881038
references.citation: Alcohol. 2002 Feb;26(2):103-13
references.pmid: 12007585
references.citation: Int J Addict. 1989 Jan;24(1):19-28
references.pmid: 2759762
references.citation: Behav Pharmacol. 2006 Nov;17(7):605-14
references.pmid: 17021393
references.citation: Alcohol Clin Exp Res. 2010 Apr;34(4):734-42
references.pmid: 20102570
references.citation: Am J Physiol Endocrinol Metab. 2007 Aug;293(2):E531-7
references.pmid: 17488809
references.citation: Alcohol Clin Exp Res. 1986 Aug;10(4):436-42
references.pmid: 3530023
references.citation: Pharmacol Biochem Behav. 2008 Apr;89(2):137-44
references.pmid: 18207224
references.citation: Psychopharmacology (Berl). 2009 Oct;206(2):303-12
references.pmid: 19629450
references.citation: Endocrinology. 1975 Oct;97(4):898-907
references.pmid: 1193012
references.citation: Dev Psychobiol. 1986 Sep;19(5):427-38
references.pmid: 3758488
references.citation: Alcohol. 2008 Dec;42(8):617-21
references.pmid: 19038695
references.citation: Brain Res. 1988 Mar 22;444(2):340-5
references.pmid: 2834024
references.citation: Cereb Cortex. 2000 Oct;10(10):1014-27
references.pmid: 11007552
references.citation: J Clin Invest. 1998 Jun 15;101(12):2677-85
references.pmid: 9637701
references.citation: Brain Res Dev Brain Res. 1997 Mar 17;99(1):38-52
references.pmid: 9088564
references.citation: J Neurochem. 2009 Feb;108(4):920-31
references.pmid: 19077056
references.citation: Arch Gen Psychiatry. 2007 Jul;64(7):830-42
references.pmid: 17606817
references.citation: J Subst Abuse. 1997;9:103-10
references.pmid: 9494942
references.citation: Neuroscience. 2008 Jul 17;154(4):1440-9
references.pmid: 18554817
references.citation: J Comp Neurol. 1996 Mar 04;366(2):223-30
references.pmid: 8698883
references.citation: Alcohol Clin Exp Res. 2007 Dec;31(12):2020-7
references.pmid: 18034694
references.citation: Proc Natl Acad Sci U S A. 1994 Jan 4;91(1):395-9
references.pmid: 8278400
references.citation: Alcohol Clin Exp Res. 1999 Aug;23(8):1331-5
references.pmid: 10470975
references.citation: Alcohol Clin Exp Res. 2007 May;31(5):895-900
references.pmid: 17391340
references.citation: Alcohol. 2010 Feb;44(1):99-110
references.pmid: 20113878
references.citation: Alcohol Clin Exp Res. 1996 Sep;20(6):1043-9
references.pmid: 8892526
references.citation: Brain Res Bull. 1997;43(4):411-6
references.pmid: 9241444
references.citation: Psychopharmacologia. 1975 Sep 17;43(3):245-54
references.pmid: 1237914