Evidence of the E*-E equilibrium from rapid kinetics of Na+ binding to activated protein C and factor Xa.
Na(+) binding to thrombin enhances the procoagulant and prothrombotic functions of the enzyme and obeys a mechanism that produces two kinetic phases: one fast (in the microsecond time scale) due to Na(+) binding to the low activity form E to produce the high activity form E:Na(+) and another considerably slower (in the millisecond time scale) that reflects a pre-equilibrium between E and the inactive form E*. In this study, we demonstrate that this mechanism also exists in other Na(+)-activated clotting proteases like factor Xa and activated protein C. These findings, along with recent structural data, suggest that the E*-E equilibrium is a general feature of the trypsin fold.
Vogt
Austin D
AD
Department of Biochemistry and Molecular Biology, Saint Louis University School of Medicine, St. Louis, Missouri 63104, USA.
Bah
Alaji
A
Di Cera
Enrico
E
D015951:Factor Xa / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D011485:Protein Binding; D011486:Protein C / Q000737:chemistry / Q000378:metabolism*; D012964:Sodium / Q000737:chemistry* / Q000378:metabolism; D013816:Thermodynamics
D011486:Protein C; D012964:Sodium; D015951:Factor Xa
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