Osteoinductive silk-silica composite biomaterials for bone regeneration.
Osteoinductive and biodegradable composite biomaterials for bone regeneration were prepared by combining silk fibroin with silica particles. The influence of these composite systems on osteogenesis was evaluated with human mesenchymal stem cells (hMSCs) subjected to osteogenic differentiation. hMSCs adhered, proliferated, and differentiated towards osteogenic lineages on silk/silica films. The addition of the silica to the silk films influenced gene expression leading to upregulation of bone sialoprotein (BSP) and collagen type 1 (Col 1) osteogenic markers. Evidence for early bone formation in the form of collagen fibers and apatite nodules was obtained on the silk/silica films. Collagen fibers were closely associated with apatite deposits and overall collagen content was higher for the silica containing samples. Also, smaller sized silica particles (24 nm-2 μm) with large surface area facilitated silica biodegradation in vitro through particle dissolution, leading to ∼5-fold decrease in silica content over 10 weeks. These results indicate the suitability of silk/silica composite system towards bone regeneration, where degradation/remodeling rates of the organic and inorganic components can be controlled.
Mieszawska
Aneta J
AJ
Department of Biomedical Engineering, 4 Colby Street, Tufts University, Medford, MA 02155, USA.
Fourligas
Nikolaos
N
Georgakoudi
Irene
I
Ouhib
Nadia M
NM
Belton
David J
DJ
Perry
Carole C
CC
Kaplan
David L
DL
D001672:Biocompatible Materials / Q000494:pharmacology*; D001861:Bone Regeneration / Q000187:drug effects*; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003094:Collagen / Q000378:metabolism; D005109:Extracellular Matrix / Q000187:drug effects / Q000378:metabolism; D020869:Gene Expression Profiling; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D010012:Osteogenesis / Q000187:drug effects* / Q000235:genetics; D012822:Silicon Dioxide / Q000494:pharmacology*; D047011:Silk / Q000494:pharmacology*
D001672:Biocompatible Materials; D047011:Silk; D012822:Silicon Dioxide; D003094:Collagen
Biomaterials. 1990 Aug;11(6):430-4
2207234
Biomacromolecules. 2005 Nov-Dec;6(6):3100-6
16283733
Biomaterials. 2006 Sep;27(25):4434-42
16677707
Gravit Space Biol Bull. 1999 May;12(2):15-26
11541779
J R Soc Interface. 2009 Jun 6;6 Suppl 3:S349-60
19158015
Trends Mol Med. 2009 Sep;15(9):417-29
19740701
Biomacromolecules. 2004 May-Jun;5(3):786-92
15132662
Biomaterials. 2006 Dec;27(36):6064-82
16890988
Biomaterials. 2003 Aug;24(18):3079-85
12895580
Biomacromolecules. 2002 Nov-Dec;3(6):1233-9
12425660
Prog Polym Sci. 2007;32(8-9):991-1007
19543442
Biomaterials. 2008 Jul;29(19):2829-38
18417206
Calcif Tissue Int. 2006 Nov;79(5):340-53
17115241
Biomaterials. 2003 Jun;24(13):2161-75
12699652
Biomaterials. 2003 Feb;24(3):401-16
12423595
J Biomed Mater Res A. 2008 Sep;86(3):678-84
18022802
J Biomed Mater Res A. 2008 Aug;86(2):448-58
17975813
Anat Rec. 1989 Jun;224(2):139-53
2672881
Biomaterials. 2006 Feb;27(6):875-84
16102817
Tissue Eng. 2002 Apr;8(2):321-31
12031120
Eur Cell Mater. 2008 Apr 01;15:53-76
18382990
Soft Matter. 2007 Jun 19;3(7):877-882
32900081
J Biomed Mater Res. 2001 Jan;54(1):139-48
11077413
J Biomed Mater Res A. 2006 Dec 1;79(3):698-705
16850456
J Biomed Mater Res A. 2009 Feb;88(2):401-8
18306281
J Struct Biol. 1996 Jul-Aug;117(1):24-35
8776885
Med Res Rev. 2004 Sep;24(5):621-38
15224383
J Biomed Mater Res B Appl Biomater. 2006 May;77(2):431-46
16333845
J Appl Phys. 2009 May 15;105(10):102042
24803683
J Phys Chem B. 2006 Nov 2;110(43):21630-8
17064118
Int J Nanomedicine. 2010 Apr 07;5:231-7
20463939
Biomed Mater Eng. 2005;15(1-2):43-50
15623929
Biomaterials. 2006 May;27(15):2934-43
16436294
Biomaterials. 2008 Aug-Sep;29(24-25):3415-28
18502501
Biomaterials. 2007 Dec;28(35):5271-9
17727944
Biomaterials. 2005 Jun;26(17):3385-93
15621227
Clin Biochem. 1996 Jun;29(3):225-9
8740508
Biomaterials. 2005 Jun;26(18):3873-9
15626435
Biomaterials. 2003 Jan;24(2):357-65
12419638
Biochemistry. 2000 Oct 17;39(41):12739-46
11027155
Acta Biomater. 2009 Jan;5(1):115-23
18835230