Proteomics of apoptosis of multiple myeloma cells induced by proteasome inhibitor PS-341.
OBJECTIVE
To compare the proteome difference between multiple myeloma cell line U266 cells treated and untreated with PS-341, to investigate the potential drug targets, and to provide theoretical evidence for clinical therapy of multiple myeloma.


METHODS
Two-dimensional gel electrophoresis (2-DE) was performed to separate proteins from treated and untreated U266 cells with proteasome inhibitor PS-341. ImageMaster 2D Platinum software was used to analyze 2-DE image, and matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) was used to identify the differentially expressed proteins. The expression levels of differential protein BAG-2 in the 2 groups of U266 cells lines were detected by Western blot.


RESULTS
The 2-DE reference pattern of treated and untreated U266 cells with PS-341 was established. A total of 31 differential proteins were identified by MALDI-TOF-MS, 27 of which were down-regulated after PS-341 treatment. The differential expression level of BAG-2 in the 2 groups of U266 cells was confirmed by Western blot.


CONCLUSIONS
Some down-regulated proteins may be the potential drug targets of proteasome inhibitor PS-341.
Jia
Haitao
H
Institute of Life and Health Engineering, Jinan University, Guangzhou 510632, China.
Ge
Feng
F
Lu
Xinpeng
X
Zeng
Huilan
H
Li
Liping
L
Chen
Zhipeng
Z
Lu
Chunhua
C
D017209:Apoptosis / Q000187:drug effects*; D001897:Boronic Acids / Q000494:pharmacology*; D000069286:Bortezomib; D045744:Cell Line, Tumor; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D009101:Multiple Myeloma / Q000235:genetics / Q000473:pathology*; D009363:Neoplasm Proteins / Q000032:analysis; D011480:Protease Inhibitors / Q000494:pharmacology; D061988:Proteasome Inhibitors; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods; D011719:Pyrazines / Q000494:pharmacology*
D001897:Boronic Acids; D009363:Neoplasm Proteins; D011480:Protease Inhibitors; D061988:Proteasome Inhibitors; D020543:Proteome; D011719:Pyrazines; D000069286:Bortezomib