title: ATP synthases: cellular nanomotors characterized by LILBID mass spectrometry.
abstract: Mass spectrometry of membrane protein complexes is still a methodological challenge due to hydrophobic and hydrophilic parts of the species and the fact that all subunits are bound non-covalently together. The present study with the novel laser induced liquid bead ion desorption mass spectrometry (LILBID-MS) reports on the determination of the subunit composition of the F(1)F(o)-ATP synthase from Bacillus pseudofirmus OF4, that of both bovine heart and, for the first time, of human heart mitochondrial F(1)F(o)-ATP synthases. Under selected buffer conditions the mass of the intact F(1)F(o)-ATP synthase of B. pseudofirmus OF4 could be measured, allowing the analysis of complex subunit stoichiometry. The agreement with theoretical masses derived from sequence databases is very good. A comparison of the ATP synthase subunit composition of 5 different ATPases reveals differences in the complexity of eukaryotic and bacterial ATP synthases. However, whereas the overall construction of eukaryotic enzymes is more complex than the bacterial ones, functionally important subunits are conserved among all ATPases.
authors.affiliation: Institute for Physical and Theoretical Chemistry, Cluster of Excellence Frankfurt Macromolecular Complexes, Centre for Membrane Proteomics, Goethe-Universität, Max-von-Laue Str. 7, 60438 Frankfurt am Main, Germany.
authors.forename: Jan
authors.initials: J
authors.lastname: Hoffmann
authors.forename: Lucie
authors.initials: L
authors.lastname: Sokolova
authors.forename: Laura
authors.initials: L
authors.lastname: Preiss
authors.forename: David B
authors.initials: DB
authors.lastname: Hicks
authors.forename: Terry A
authors.initials: TA
authors.lastname: Krulwich
authors.forename: Nina
authors.initials: N
authors.lastname: Morgner
authors.forename: Ilka
authors.initials: I
authors.lastname: Wittig
authors.forename: Hermann
authors.initials: H
authors.lastname: Schägger
authors.forename: Thomas
authors.initials: T
authors.lastname: Meier
authors.forename: Bernd
authors.initials: B
authors.lastname: Brutschy
mesh_terms: D000818:Animals; D001407:Bacillus / Q000201:enzymology; D002417:Cattle; D006801:Humans; D013058:Mass Spectrometry; D008928:Mitochondria / Q000201:enzymology; D021122:Protein Subunits / Q000737:chemistry; D006180:Proton-Translocating ATPases / Q000737:chemistry*
chemical_list: D021122:Protein Subunits; D006180:Proton-Translocating ATPases
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