Methods to assess stem cell lineage, fate and function.
Stem cell therapy has the potential to regenerate injured tissue. For stem cells to achieve their full therapeutic potential, stem cells must differentiate into the target cell, reach the site of injury, survive, and engraft. To fully characterize these cells, evaluation of cell morphology, lineage specific markers, cell specific function, and gene expression must be performed. To monitor survival and engraftment, cell fate imaging is vital. Only then can organ specific function be evaluated to determine the effectiveness of therapy. In this review, we will discuss methods for evaluating the function of transplanted cells for restoring the heart, nervous system, and pancreas. We will also highlight the specific challenges facing these potential therapeutic areas.
Nguyen
Patricia K
PK
Department of Medicine, Stanford University School of Medicine, CA 94305, USA.
Nag
Divya
D
Wu
Joseph C
JC
D000818:Animals; D002454:Cell Differentiation; D019070:Cell Lineage; D006331:Heart Diseases / Q000378:metabolism / Q000628:therapy; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D006801:Humans; D009422:Nervous System Diseases / Q000378:metabolism / Q000628:therapy; D010182:Pancreatic Diseases / Q000378:metabolism / Q000628:therapy; D012038:Regeneration; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000502:physiology*
Nat Protoc. 2006;1(6):3069-75
17406570
Circulation. 2006 Mar 14;113(10):1287-94
16520413
Gene Ther. 1997 Oct;4(10):1013-22
9415306
Physiol Genomics. 2006 Mar 13;25(1):29-38
16390873
PLoS One. 2010 Feb 01;5(2):e8975
20126639
NMR Biomed. 2004 Nov;17(7):513-7
15526348
J Magn Reson Imaging. 2007 Sep;26(3):720-7
17729351
Nat Biotechnol. 2007 Sep;25(9):1015-24
17721512
Bioconjug Chem. 2009 Apr;20(4):702-9
19331388
Mol Imaging Biol. 2009 May-Jun;11(3):178-87
19034584
Circ Cardiovasc Imaging. 2008 Sep;1(2):94-103
19808526
Circ Res. 2002 Aug 9;91(3):189-201
12169644
Nat Biotechnol. 2006 Nov;24(11):1392-401
17053790
Diabetes. 2004 Oct;53(10):2603-9
15448090
Genes Dev. 2003 Mar 1;17(5):545-80
12629038
J Nucl Cardiol. 2004 Jul-Aug;11(4):491-505
15295418
J Neurosci. 2006 Nov 29;26(48):12497-511
17135412
Proc Natl Acad Sci U S A. 2002 Dec 10;99(25):16267-72
12444255
Nature. 2006 Jun 29;441(7097):1094-6
16810245
Nat Biotechnol. 2000 Apr;18(4):410-4
10748521
Thromb Haemost. 2010 Jul;104(1):13-22
20458434
Nucl Med Biol. 2003 Nov;30(8):889-95
14698793
J Nucl Med. 2004 Mar;45(3):512-8
15001696
Neuropathology. 2006 Apr;26(2):129-40
16708545
JAMA. 2009 Apr 15;301(15):1573-9
19366777
Curr Biol. 2001 Apr 3;11(7):514-8
11413002
Proc Natl Acad Sci U S A. 2006 Jul 25;103(30):11294-9
16868090
Nature. 2004 Apr 8;428(6983):668-73
15034594
Circulation. 2008 Sep 30;118(14 Suppl):S121-9
18824743
Nat Protoc. 2009;4(8):1192-201
19617890
Proc Natl Acad Sci U S A. 2007 Jul 17;104(29):12175-80
17586681
J Neuropathol Exp Neurol. 2001 Aug;60(8):741-52
11487048
Phys Med Biol. 2003 Jun 7;48(11):1519-37
12817935
Nature. 2002 Jul 4;418(6893):50-6
12077607
J Am Coll Cardiol. 2007 Mar 20;49(11):1137-48
17367656
Circulation. 2006 Feb 21;113(7):1005-14
16476845
Science. 2001 May 18;292(5520):1389-94
11326082
Nat Med. 2004 May;10(5):494-501
15107841
FASEB J. 2009 Dec;23(12):4168-80
19703934
Am J Cardiol. 2004 Jul 1;94(1):92-5
15219514
J Nucl Cardiol. 2008 May-Jun;15(3):308-10
18513636
Circ Res. 2009 Feb 13;104(3):398-402
19096027
Circulation. 2005 Aug 30;112(9 Suppl):I178-83
16159812
Nat Protoc. 2006;1(2):495-507
17406275
J Clin Endocrinol Metab. 2010 Mar;95(3):1034-43
20061422
Neuroscience. 2007 Oct 12;149(1):38-52
17870247
Mol Cells. 2007 Apr 30;23(2):132-7
17464188
PLoS One. 2008;3(10):e3474
18941512
Cell Mol Biol Lett. 2009;14(1):100-12
18839068
Proc Natl Acad Sci U S A. 2010 Mar 2;107(9):4335-40
20160098
Circulation. 2003 Aug 19;108(7):863-8
12900340
Stroke. 2007 Mar;38(3):1031-5
17272763
Eur J Nucl Med Mol Imaging. 2006 Oct;33(10):1171-7
16763813
Science. 2003 Jan 17;299(5605):363
12532008
Nature. 2008 Feb 21;451(7181):937-42
18288183
Circ Res. 2009 Feb 27;104(4):e30-41
19213953
Stem Cells. 2008 Apr;26(4):864-73
18218820
J Nucl Med Technol. 2004 Jun;32(2):47-57; quiz 58-9
15175400
Stem Cells. 2007 Oct;25(10):2677-84
17628019
Circulation. 2003 Sep 16;108(11):1302-5
12963637
Nat Clin Pract Cardiovasc Med. 2006 Oct;3(10):554-62
16990841
J Neurosci. 2004 Jun 30;24(26):5982-6002
15229246
Circulation. 2007 Sep 11;116(11 Suppl):I46-54
17846325
N Engl J Med. 2006 Sep 21;355(12):1222-32
16990385
Stem Cells. 2008 Jul;26(7):1831-40
18436862
PLoS One. 2007 Dec 05;2(12):e1272
18060066
Nature. 2003 Oct 30;425(6961):968-73
14555960
PLoS One. 2007 Jan 17;2(1):e156
17225860
Cancer Res. 2003 Apr 15;63(8):1936-42
12702586
Proteomics. 2006 Dec;6(23):6234-49
17080479
Magn Reson Med. 2004 Nov;52(5):1214-9
15508153
Proc Natl Acad Sci U S A. 2003 Mar 4;100(5):2397-402
12606728
Nature. 2010 Sep 16;467(7313):285-90
20644535
Stem Cells. 2007 May;25(5):1204-12
17218400
Am Heart J. 2006 Oct;152(4):684.e11-6
16996834
Mol Ther. 2009 Jul;17(7):1282-91
19436269
Diabetes. 2006 Sep;55(9):2419-28
16936189
J Nucl Med. 2007 Dec;48(12):1916-9
18056330
Nature. 2010 Feb 25;463(7284):1035-41
20107439
Proc Natl Acad Sci U S A. 2002 Dec 10;99(25):16105-10
12441403
Circulation. 2003 Apr 29;107(16):2134-9
12695305
Curr Opin Chem Biol. 2010 Feb;14(1):71-9
19879798