Condensed E. coli cultures for highly efficient production of proteins containing unnatural amino acids.
Current biosynthetic methods for producing proteins containing site-specifically incorporated unnatural amino acids are inefficient because the majority of the amino acid goes unused. Here we present a universal approach to improve the efficiency of such processes using condensed Escherichia coli cultures.
Liu
Jia
J
Department of Chemistry and Biochemistry, University of Maryland, College Park, MD 20742, USA.
Castañeda
Carlos A
CA
Wilkins
Bryan J
BJ
Fushman
David
D
Cropp
T Ashton
TA
D000596:Amino Acids / Q000378:metabolism*; D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism; D001577:Benzophenones / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism*; D010649:Phenylalanine / Q000031:analogs & derivatives / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000737:chemistry; D025801:Ubiquitin / Q000096:biosynthesis
C488060:4-benzoylphenylalanine; D000596:Amino Acids; D001577:Benzophenones; D011994:Recombinant Proteins; D025801:Ubiquitin; D010649:Phenylalanine; D000604:Amino Acyl-tRNA Synthetases
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