title: [HCV DF protein inhibits expression of p16 and p21 in HepG₂ cells].
abstract: OBJECTIVE
  To investigate the effect of HCV DF (Double-shift F) protein on the expression p16 and p21 in HepG(2); cells.
  
  
  METHODS
  DF gene was amplificated from the whole HCV 1b genome, and cloned into pCDNA3.0 vecter. The recombinant plasmid (pCDNA3.0/HCV-DF) and empty vector were transfected into HepG(2); cells. Screening was performed with G418. p16 and p21 mRNA were detected by semi-quantitative RT-PCR, and protein by Western blot.
  
  
  RESULTS
  Stable expression of the recombinant plasmid was found in HCV DF protein. The expression of p16 and p21 in HepG();2 cells transfected with pCDNA3.0/HCV-DF were lower than those with blank plasmid.
  
  
  CONCLUSIONS
  HCV DF protein inhibits expression of p16 and p21 in HepG(2); cells. This suggested that HCV DF protein may participate in the progress of hepatocellular carcinoma.
authors.affiliation: Department of Public Health, Nanjing Medical University, Nanjing 210029, China.
authors.forename: Hao-Peng
authors.initials: HP
authors.lastname: Wang
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhang
authors.forename: Jing-Jing
authors.initials: JJ
authors.lastname: Yang
authors.forename: Jie
authors.initials: J
authors.lastname: Wang
authors.forename: Xiao-Zhao
authors.initials: XZ
authors.lastname: Deng
authors.forename: Ke
authors.initials: K
authors.lastname: Xu
mesh_terms: D015153:Blotting, Western; D006528:Carcinoma, Hepatocellular / Q000473:pathology*; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D019942:Genes, p16 / Q000187:drug effects* / Q000502:physiology; D005822:Genetic Vectors; D016174:Hepacivirus / Q000737:chemistry* / Q000235:genetics; D006801:Humans; D010957:Plasmids / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection; D014758:Viral Core Proteins / Q000494:pharmacology*; D020741:rho GTP-Binding Proteins / Q000187:drug effects* / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D014758:Viral Core Proteins; D020741:rho GTP-Binding Proteins