Joostella atrarenae sp. nov., a novel member of the Flavobacteriaceae originating from the black sea sand of Jeju Island.
Strain M1-2(T) was isolated from the black sand from the seashore of Jeju Island, Republic of Korea and was classified using a polyphasic taxonomic approach. Strain M1-2(T) appeared as Gram-negative, motile rods that could grow in the presence of 1-10% (w/v) NaCl and at temperatures ranging from 4 to 37°C. This isolate has catalase and oxidase activity and hydrolyses aesculin, DNA and L: -tyrosine. Based on phylogenetic analysis using 16S rRNA gene sequences, strain M1-2(T) belongs to the genus Joostella and is clearly distinct from the other described species of this genus, Joostella marina (type strain En5(T)). The 16S rRNA gene sequence similarity level between M1-2(T) and J. marina En5(T) is 97.2%, and the DNA-DNA relatedness value between the two strains is 23.9%. Strain M1-2(T) contains MK-6 as the major menaquinone and iso-C₁₅:₀, summed feature 3 (C₁₆:₁ ω7c and/or iso-C₁₅:₀ 2OH) and iso-C₁₇:₀ 3OH as major cellular fatty acids. The DNA G + C content is 32.3 mol%. These data suggest that strain M1-2(T) should be classified as a novel species, for which the name Joostella atrarenae sp. nov. is proposed. The type strain for the novel species is M1-2(T) (= KCTC 23194(T) = NCAIM B.002413(T)).
Kim
Byung-Chun
BC
Biological Resource Center, Korea Research Institute of Bioscience & Biotechnology, Daejeon, Korea.
Lee
Kang Hyun
KH
Kim
Mi Na
MN
Jung
Min Young
MY
Chang
Young-Hyo
YH
Lee
Jongtae
J
Shin
Kee-Sun
KS
D015373:Bacterial Typing Techniques; D001482:Base Composition; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D041965:Flavobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D019015:Geologic Sediments / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D012822:Silicon Dioxide
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D012822:Silicon Dioxide
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