title: Optimization of enantioselective synthesis of methyl (R)-2-chloromandelate by whole cells of Saccharomyces cerevisiae.
abstract: Methyl (R)-2-chloromandelate, a key intermediate in the synthesis of clopidogrel, was obtained by the reduction of methyl-2-chlorobenzoylformate using whole cells of Saccharomyces cerevisiae. A 100% conversion and 96.1% of enantiomeric excess (ee) value was obtained when 17 methyl-2-chlorobenzoylformate/l was reacted with 8 g S. cerevisiae/l and 83 g glucose/l at pH 7.
authors.affiliation: School of Chemical Engineering and Bioengineering, University of Ulsan, 29 Mugeo-dong Nam-gu, Ulsan, 680-749, Republic of Korea.
authors.forename: Min
authors.initials: M
authors.lastname: Jeong
authors.forename: Yoon Mi
authors.initials: YM
authors.lastname: Lee
authors.forename: Soon Ho
authors.initials: SH
authors.lastname: Hong
authors.forename: Sung Young
authors.initials: SY
authors.lastname: Park
authors.forename: Ik-keun
authors.initials: IK
authors.lastname: Yoo
authors.forename: Mee Jung
authors.initials: MJ
authors.lastname: Han
mesh_terms: D001711:Biotransformation; D005947:Glucose / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008333:Mandelic Acids / Q000737:chemistry / Q000378:metabolism*; D010648:Phenylacetates / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D013237:Stereoisomerism
chemical_list: D008333:Mandelic Acids; D010648:Phenylacetates; C522563:methyl 2-chlorobenozylformate; C522562:methyl 2-chloromandelate; D005947:Glucose