title: A duplex PCR method to identify mosaic penA gene and predict reduced susceptibility to oral cephalosporins in Neisseria gonorrhoeae.
abstract: Neisseria gonorrhoeae with mosaic penicillin-binding protein 2 (PBP2) is associated with reduced susceptibility to third-generation oral cephalosporins. A simple and rapid PCR method using three primers was designed to identify mosaic PBP2, which could help predict reduced susceptibility to expanded-spectrum cephalosporins in N. gonorrhoeae.
authors.affiliation: Research and Diagnostic Center, Centers for Disease Control, Taipei, Taiwan.
authors.forename: Chung-Ter
authors.initials: CT
authors.lastname: Huang
authors.forename: Jennifer
authors.initials: J
authors.lastname: Niu
authors.forename: Mei-Hui
authors.initials: MH
authors.lastname: Liao
authors.forename: Shu-Ying
authors.initials: SY
authors.lastname: Li
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002511:Cephalosporins / Q000494:pharmacology*; D017931:DNA Primers / Q000235:genetics; D005838:Genotype; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods; D009344:Neisseria gonorrhoeae / Q000187:drug effects* / Q000235:genetics*; D046915:Penicillin-Binding Proteins / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; D002511:Cephalosporins; D017931:DNA Primers; D046915:Penicillin-Binding Proteins