title: Mutations in the beta-myosin heavy chain gene in southern Chinese families with hypertrophic cardiomyopathy.
abstract: In this study, 14 unrelated hypertrophic cardiomyopathy (HCM) probands were scanned by polymerase chain reaction-single-strand conformation polymorphism analysis and DNA sequencing. Three mis-sense mutations of the beta-myosin heavy chain gene, MYH7, were found: valine (Val) 606 methionine (Met), arginine (Arg) 694 leucine (Leu), and Arg 723 glycine (Gly). All are reported here for the first time in Chinese subjects. The results showed that: Val606Met is an intermediate malignancy mutation; Arg694Leu is a novel mutation with a benign phenotype; and the Arg723Gly mutation is linked to malignancy - it can lead not only to HCM but also to dilated cardiomyopathy at various ages. The clinical symptoms associated with Arg723Gly emerged early and caused more severe clinical manifestation and poorer prognosis in females than in males. Mis-sense mutations were not detected in the myosin binding protein C, cardiac, cardiac troponin T type 2, or cardiac troponin I type 3 genes. The MYH7 gene may be an HCM mutation hotspot in the Chinese and have unique features in this study population.
authors.affiliation: Department of Cardiology, First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China.
authors.forename: D D
authors.initials: DD
authors.lastname: Zheng
authors.forename: J H
authors.initials: JH
authors.lastname: Yang
authors.forename: Q
authors.initials: Q
authors.lastname: Tao
authors.forename: M
authors.initials: M
authors.lastname: Geng
authors.forename: J
authors.initials: J
authors.lastname: Lin
authors.forename: X J
authors.initials: XJ
authors.lastname: Yang
authors.forename: J P
authors.initials: JP
authors.lastname: Song
authors.forename: H X
authors.initials: HX
authors.lastname: Li
authors.forename: L H
authors.initials: LH
authors.lastname: Han
authors.forename: W P
authors.initials: WP
authors.lastname: Jiang
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D024722:Cardiac Myosins / Q000235:genetics*; D024741:Cardiomyopathy, Hypertrophic, Familial / Q000208:ethnology / Q000473:pathology*; D002681:China / Q000208:ethnology; D017124:Conserved Sequence; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation; D018995:Myosin Heavy Chains / Q000235:genetics*; D010375:Pedigree; D018807:Polymorphism, Single-Stranded Conformational; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D055815:Young Adult
chemical_list: C501641:MYH7 protein, human; D024722:Cardiac Myosins; D018995:Myosin Heavy Chains