Interlaboratory evaluation of different extraction and real-time PCR methods for detection of Coxiella burnetii DNA in serum.
In the Netherlands, there is an ongoing and unparalleled outbreak of Q fever. Rapid and reliable methods to identify patients infected with Coxiella burnetii, the causative agent of Q fever, are urgently needed. We evaluated the performance of different DNA extraction methods and real-time PCR assays that are in use in seven diagnostic or reference laboratories in the Netherlands. A low degree of variation in the sensitivities of most of the developed real-time PCR assays was observed. However, PCR assays amplifying short DNA fragments yielded better results than those producing large DNA fragments. With regard to DNA extraction, the automated MagNA Pure Compact system and the manual QIAamp DNA mini kit consistently yielded better results than either the MagNA Pure LC system and NucliSens EasyMag (both automated) or the High Pure viral nucleic acid kit (manual). The present study shows that multiple combinations of DNA extraction kits and real-time PCR assays offer equivalent solutions to detect C. burnetii DNA in serum samples from patients suspected to have Q fever.
Tilburg
Jeroen J H C
JJ
Department of Medical Microbiology and Infectious Diseases, Canisius Wilhelmina Hospital, Nijmegen, Netherlands.
Melchers
Willem J G
WJ
Pettersson
Annika M
AM
Rossen
John W A
JW
Hermans
Mirjam H A
MH
van Hannen
Erik J
EJ
Nabuurs-Franssen
Marrigje H
MH
de Vries
Maaike C
MC
Horrevorts
Alphons M
AM
Klaassen
Corné H W
CH
D001431:Bacteriological Techniques / Q000379:methods*; D016997:Coxiella burnetii / Q000235:genetics / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000235:genetics* / Q000302:isolation & purification*; D006801:Humans; D009426:Netherlands; D016133:Polymerase Chain Reaction / Q000379:methods*; D011778:Q Fever / Q000175:diagnosis* / Q000382:microbiology; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D044967:Serum / Q000382:microbiology*
D004269:DNA, Bacterial
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