E-selectin gene polymorphisms are associated with essential hypertension: a case-control pilot study in a Chinese population.
BACKGROUND
Genetic variation is thought to contribute to the etiology of hypertension, and E-selectin is a candidate essential hypertension-associated gene. This study thus sought to investigate possible genetic associations between the T1880C, C602A and T1559C polymorphisms of E-selectin and essential hypertension.


METHODS
Hypertensive patients (n = 490) and healthy normotensive subjects (n = 495) were screened for the genotypes T1880C, C602A and T1559C using real-time quantitative polymerase chain reaction after DNA extraction to identify representative variations in the E-selectin gene. The associations between genotypes and alleles of the three mutations and essential hypertension were then analyzed using a case-control study.


RESULTS
Hypertensive patients and normotensive subjects were significantly different with respect to the genotypes CC, CA and AA (P = 0.005) and the C-allele frequency of C602A (P = 0.001). A comparison of dominant versus recessive models also revealed significant differences between the two groups (P = 0.004 and P = 0.02). When subgrouped by gender, these indexes differed significantly between normotensive and essential hypertensive males, but not in females. The additive model of the T1559C genotype did not differ between essential hypertensive and normotensive groups overall (P = 0.39), but it was different between hypertensive and normotensive males (P = 0.046) and females (P = 0.045). The CC + TC versus TT frequency of T1559C was also different in the recessive model of male hypertensive and normotensive groups (P = 0.02). Further analysis showed that C602A and T1559C were significantly associated with hypertension (C602A: OR = 7.58, 95%CI = 1.53-11.97, P < 0.01; and T1559C: OR = 6.77, 95%CI = 1.07-1.83, P < 0.05). The frequency of the C-C-C haplotype was significantly higher in hypertensive patients than in control individuals as well as in hypertensive and normotensive males (P = 0.008 and 0.01). The frequency of the C-A-T haplotype was higher only in male hypertensives and normotensives (P = 0.015). Furthermore, there was a significant interaction between E-selectin and gender (P = 0.02 for C602A and 0.04 for T1559C).


CONCLUSIONS
C602A and T1559C may be independent risk factors for essential hypertension in the Chinese population, whereas T1880C is not.
Wang
Zuoguang
Z
Department of Hypertension, Beijing Anzhen Hospital, Attached to Capital University of Medical Sciences, Beijing Institute of Heart, Lung, Blood Vessel Diseases, Beijing, 100029, China.
Liu
Ya
Y
Liu
Jieling
J
Liu
Kuo
K
Lou
Yuqin
Y
Wen
Jie
J
Niu
Qiuli
Q
Wen
Shaojun
S
Wu
Zhaosu
Z
D000483:Alleles; D044466:Asian People / Q000235:genetics; D016022:Case-Control Studies; D002681:China; D019040:E-Selectin / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D006973:Hypertension / Q000208:ethnology / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D012737:Sex Factors
D019040:E-Selectin
Clin Exp Hypertens. 2009 Jun;31(4):316-29
19811360
Cerebrovasc Dis. 2009;28(1):26-32
19420919
Eur J Gastroenterol Hepatol. 2009 Feb;21(2):167-75
19212205
J Hum Hypertens. 2007 Apr;21(4):333-6
17273158
Scand Cardiovasc J. 2007 Oct;41(5):313-20
17852781
Thromb Res. 2003;112(1-2):47-50
15013273
Minerva Cardioangiol. 2009 Dec;57(6):687-704
19942842
J Neurol Sci. 2009 Oct 15;285(1-2):137-41
19596363
Nat Genet. 1999 Jul;22(3):239-47
10391210
Scand J Clin Lab Invest. 2006;66(6):523-34
17000560
Hypertension. 2003 Jan;41(1):25-30
12511525
Int J Cardiol. 2005 Jan;98(1):39-44
15676164
J Hypertens. 2004 Feb;22(2):305-11
15076188
Hypertension. 2007 Jan;49(1):96-106
17159089
BMC Med Genet. 2006 Jun 06;7:52
16756647
J Int Med Res. 2009 Jul-Aug;37(4):996-1002
19761681
J Reprod Med. 2007 Sep;52(9):815-8
17939599
J Immunol. 2003 Sep 15;171(6):3216-24
12960351
J Cardiovasc Surg (Torino). 2004 Apr;45(2):143-7
15179350
Hypertension. 2004 Nov;44(5):668-73
15466663
Kidney Blood Press Res. 2009;32(2):106-9
19342863
Circulation. 2006 Jul 4;114(1 Suppl):I275-81
16820586
BMC Med Genet. 2009 Dec 09;10:130
20003179