Crystallization and preliminary X-ray analysis of a glucansucrase from the dental caries pathogen Streptococcus mutans.
Glucansucrases encoded by Streptococcus mutans play essential roles in the synthesis of sticky dental plaques. Based on amino-acid sequence similarity, glucansucrases are classified as members of glycoside hydrolase family 70 (GH 70). Data on the crystal structure of GH 70 glucansucrases have yet to be reported. Here, the GH 70 glucansucrase GTF-SI from S. mutans was overexpressed in Escherichia coli strain BL21 (DE3), purified to homogeneity and crystallized using the hanging-drop vapour-diffusion method. Orthorhombic GTF-SI crystals belonging to space group P2(1)2(1)2 were obtained. A diffraction data set was collected to 2.1 A resolution.
Ito
Keisuke
K
Department of Food and Nutritional Sciences, Graduate School of Nutritional and Environmental Sciences, University of Shizuoka, Yada 52-1, Suruga-ku, Shizuoka 422-8526, Japan.
Ito
Sohei
S
Shimamura
Tatsuro
T
Kawarasaki
Yasuaki
Y
Abe
Keiko
K
Misaka
Takumi
T
Kobayashi
Takuya
T
Iwata
So
S
D003460:Crystallization; D018360:Crystallography, X-Ray; D016695:Glycosyltransferases / Q000737:chemistry*; D013295:Streptococcus mutans / Q000201:enzymology*
D016695:Glycosyltransferases; C033837:alternansucrase
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