title: Effect of triazophos, fipronil and their mixture on miRNA expression in adult zebrafish.
abstract: MicroRNA (miRNA) plays a crucial role in gene expression regulation. However, no data are available on change of miRNA expression of zebrafish (Danio rerio) after treatment with pesticides. We evaluated the effect of fipronil (5-amino-1-[2, 6-dichloro-4-(trifluoromethyl) phenyl]-4-[(trifluoromethyl) sulfinyl]-1H-pyrazole-3-carbonitrile) and triazophos (3-(O, O-diethyl)-1-phenyl thiophosphoryl-1, 2, 4-triazol) and their mixture on miRNA expression in zebrafish.MiRNA expression profiles in zebrafish were altered after treatment with these chemicals. An association between these chemicals and the expression of 21 miRNAs was found 96 h after treatment. Among them, 14 miRNAs were differentially expressed due to the treatments with fipronil, triazophos and their mixture; 5 miRNAs showed altered expression level after treatment with formulations of these chemicals; miR-29b and miR-738 were differentially expressed after treatment with adjuvants. MiRNAs might present a novel toxicological response that could be used as a toxicological biomarker and have a different direction for future investigations of their association with miRNAs involved in chemical related diseases.
authors.affiliation: Institute of Bioengineering, Zhejiang Sci-Tech University, Hangzhou, Zhejiang, PR China.
authors.forename: Xingxing
authors.initials: X
authors.lastname: Wang
authors.forename: Shengli
authors.initials: S
authors.lastname: Zhou
authors.forename: Xianfeng
authors.initials: X
authors.lastname: Ding
authors.forename: Guonian
authors.initials: G
authors.lastname: Zhu
authors.forename: Jiangfeng
authors.initials: J
authors.lastname: Guo
mesh_terms: D000818:Animals; D004784:Environmental Monitoring; D005786:Gene Expression Regulation / Q000187:drug effects*; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D063086:Organothiophosphates / Q000494:pharmacology*; D010575:Pesticides / Q000494:pharmacology*; D011720:Pyrazoles / Q000494:pharmacology*; D014230:Triazoles / Q000494:pharmacology*; D015027:Zebrafish / Q000235:genetics* / Q000378:metabolism
chemical_list: D035683:MicroRNAs; D063086:Organothiophosphates; D010575:Pesticides; D011720:Pyrazoles; D014230:Triazoles; C012584:triazophos; C082360:fipronil