title: [Construction of full-length cDNA library of erythrocytic stage Plasmodium vivax].
abstract: Blood samples were collected from vivax malaria patients without antimalarial drug therapy. After filtration through Plasmodipur filter to remove white blood cells, Plasmodium vivax-infected RBCs were enriched by Percoll. Total P. vivax in red blood cells was isolated. A full-length cDNA library of erythrocytic stage P. vivax was constructed by the SMART cDNA library construction kit. The volume and recombinant rate of the library were evaluated. The inserted fragments were identified by PCR amplification. The titer of cDNA library was 1.14 x 10(6). The length of inserted fragment ranged from 900 to 2 500 bp, and the recombination efficiency accounted for 97.2%.
authors.affiliation: Department of Microbiology and Parasitology, Bengbu Medical College; Anhui Key Laboratory of Infection and Immunity, Bengbu 233030, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Fang
authors.forename: Hui
authors.initials: H
authors.lastname: Xia
authors.forename: Yuan-Ying
authors.initials: YY
authors.lastname: Yuan
authors.forename: Jun
authors.initials: J
authors.lastname: Cao
authors.forename: Xue-Mei
authors.initials: XM
authors.lastname: Wang
authors.forename: Wen-Juan
authors.initials: WJ
authors.lastname: Qi
authors.forename: Qi
authors.initials: Q
authors.lastname: Gao
mesh_terms: D003001:Cloning, Molecular; D004912:Erythrocytes / Q000469:parasitology*; D015723:Gene Library; D006801:Humans; D016780:Malaria, Vivax / Q000097:blood / Q000469:parasitology; D010966:Plasmodium vivax / Q000235:genetics* / Q000302:isolation & purification