title: Alteration of cross-linking selectivity with the 2'-OMe analogue of 2-amino-6-vinylpurine and evaluation of antisense effects.
abstract: We previously reported that oligodeoxynucleotides containing 2-amino-6-vinylpurine (2-AVP: 1) exhibit efficient selective cross-linking to cytosine. In this study, the 2'-OMe nucleoside analogue (2) of 2-AVP was designed in order to increase its affinity to RNA and enhance metabolic stability. It has been demonstrated that 2'-OMe oligonucleotides bearing 2 achieve highly selective cross-linking to the thymine base in DNA and show higher antisense effect on luciferase production in cell lysate.
authors.affiliation: Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, Aoba-ku, Sendai-shi, Miyagi, Japan.
authors.forename: Shuhei
authors.initials: S
authors.lastname: Imoto
authors.forename: Tsuneaki
authors.initials: T
authors.lastname: Hori
authors.forename: Shinya
authors.initials: S
authors.lastname: Hagihara
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Taniguchi
authors.forename: Shigeki
authors.initials: S
authors.lastname: Sasaki
authors.forename: Fumi
authors.initials: F
authors.lastname: Nagatsugi
mesh_terms: D000818:Animals; D001483:Base Sequence; D003432:Cross-Linking Reagents / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D049409:Luciferases, Firefly / Q000235:genetics; D016376:Oligonucleotides, Antisense / Q000737:chemistry* / Q000235:genetics; D011687:Purines / Q000737:chemistry*; D012333:RNA, Messenger / Q000235:genetics; D013941:Thymine / Q000737:chemistry*; D014753:Vinyl Compounds / Q000737:chemistry*
chemical_list: C432203:2-amino-6-vinylpurine; D003432:Cross-Linking Reagents; D016376:Oligonucleotides, Antisense; D011687:Purines; D012333:RNA, Messenger; D014753:Vinyl Compounds; D004247:DNA; D049409:Luciferases, Firefly; D013941:Thymine