Wnt/beta-catenin is involved in the proliferation of hippocampal neural stem cells induced by hypoxia.
OBJECTIVE
Beta-catenin, as a major effector molecule in the canonical Wnt signaling pathway, could regulate adult neurogenesis. Here, the role of Wnt/β-catenin signaling pathway in the proliferation of hippocampal neural stem cells (NSCs) induced by hypoxia was investigated.


METHODS
The hippocampal NSCs of neonatal green fluorescent protein transgenic mice on day 0 were cultured in hypoxia (5% O(2)) and traditional O(2) (20% O(2)). The expression of β-catenin, p-GSK-3β, and cyclinD1 in NSCs was measured under hypoxia or traditional O(2) by western blotting. NSCs were electroporated with pTOPFLASH reporter in different conditions and the LEF/TCF-dependent luciferase activity was assayed.


RESULTS
Hypoxia increased the proliferation and reduced the apoptosis of hippocampal NSCs. NSCs proliferation was inhibited by transfecting with pAxin, whereas promoted by transfecting with pβ-catenin.


CONCLUSIONS
Hypoxia could enhance the proliferation of hippocampal NSCs and β-catenin contributed to this action.
Cui
X-P
XP
Department of Anatomy, Third Military Medical University, Chongqing, China.
Xing
Y
Y
Chen
J-M
JM
Dong
S-W
SW
Ying
D-J
DJ
Yew
D T
DT
D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism; D000818:Animals; D060466:Axin Protein; D049109:Cell Proliferation; D051157:Frizzled Receptors / Q000378:metabolism; D038362:Glycogen Synthase Kinase 3 / Q000378:metabolism; D000071679:Glycogen Synthase Kinase 3 beta; D006624:Hippocampus; D000860:Hypoxia; D051821:Lymphoid Enhancer-Binding Factor 1 / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D058953:Neural Stem Cells / Q000378:metabolism / Q000502:physiology*; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism; D012097:Repressor Proteins / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology*; D051176:beta Catenin / Q000378:metabolism / Q000502:physiology*
D048868:Adaptor Proteins, Signal Transducing; D060466:Axin Protein; D051157:Frizzled Receptors; C489739:Fzd1 protein, mouse; C497574:Lef1 protein, mouse; D051821:Lymphoid Enhancer-Binding Factor 1; D043562:Receptors, G-Protein-Coupled; D012097:Repressor Proteins; D051176:beta Catenin; D000071679:Glycogen Synthase Kinase 3 beta; C000605507:Gsk3b protein, mouse; D038362:Glycogen Synthase Kinase 3
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