title: Adhesion of fibroblasts on micro- and nanostructured surfaces prepared by chemical vapor deposition and pulsed laser treatment.
abstract: The development of micro- and nanostructured surfaces which improve the cell-substrate interaction is of great interest in today's implant applications. In this regard, Al/Al2O3 bi-phasic nanowires were synthesized by chemical vapor deposition of the molecular precursor (tBuOAlH2)2. Heat treatment of such bi-phasic nanowires with short laser pulses leads to micro- and nanostructured Al2O3 surfaces. Such surfaces were characterized by scanning electron microscopy (SEM), electron dispersive spectroscopy and x-ray photoelectron spectroscopy. Following the detailed material characterization, the prepared surfaces were tested for their cell compatibility using normal human dermal fibroblasts. While the cells cultivated on Al/Al2O3 bi-phasic nanowires showed an unusual morphology, cells cultivated on nanowires treated with one and two laser pulses exhibited morphologies similar to those observed on the control substrate. The highest cell density was observed on surfaces treated with one laser pulse. The interaction of the cells with the nano- and microstructures was investigated by SEM analysis in detail. Laser treatment of Al/Al2O3 bi-phasic nanowires is a fast and easy method to fabricate nano- and microstructured Al2O3-surfaces for studying cell-surface interactions. It is our goal to develop a biocompatible Al2O3-surface which could be used as a coating material for medical implants exhibiting a cell selective response because of its specific physical landscape and especially because it promotes the adhesion of osteoblasts while minimizing the adhesion of fibroblasts.
authors.affiliation: INM-Leibniz Institute for New Materials, Campus D2 2, 66123 Saarbrücken, Germany. Michael.Veith@inm-gmbh.de
authors.forename: M
authors.initials: M
authors.lastname: Veith
authors.forename: O C
authors.initials: OC
authors.lastname: Aktas
authors.forename: W
authors.initials: W
authors.lastname: Metzger
authors.forename: D
authors.initials: D
authors.lastname: Sossong
authors.forename: H
authors.initials: H
authors.lastname: Ullah Wazir
authors.forename: I
authors.initials: I
authors.lastname: Grobelsek
authors.forename: N
authors.initials: N
authors.lastname: Pütz
authors.forename: G
authors.initials: G
authors.lastname: Wennemuth
authors.forename: T
authors.initials: T
authors.lastname: Pohlemann
authors.forename: M
authors.initials: M
authors.lastname: Oberringer
mesh_terms: D000537:Aluminum Oxide; D000704:Analysis of Variance; D002448:Cell Adhesion / Q000502:physiology*; D002452:Cell Count; D018929:Cell Culture Techniques / Q000379:methods*; D048430:Cell Shape; D002478:Cells, Cultured; D005347:Fibroblasts / Q000502:physiology*; D006801:Humans; D007150:Immunohistochemistry; D007834:Lasers; D008422:Materials Testing / Q000379:methods*; D008855:Microscopy, Electron, Scanning; D053770:Nanowires / Q000737:chemistry*; D056951:Photoelectron Spectroscopy; D010784:Photomicrography; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods
chemical_list: D000537:Aluminum Oxide