Characterization and identification of distinct Mycobacterium massiliense extracellular proteins from those of Mycobacterium abscessus.
Mycobacterium massiliense is an emerging pathogen and very similar to Mycobacterium abscessus of rapidly growing mycobacteria in the phenotype and genotype. Pathogenic bacteria secrete a diversity of factors into extracellular medium which contribute to the bacterial pathogenicity. In the present study, we performed the comparative proteome analysis of culture filtrate proteins from a clinical isolate of M. massiliense and M. abscessus strains using two-dimensional gel electrophoresis and liquid chromatography-electrospray ionization mass spectrometry (LC-ESI-MS). Interestingly, 9 proteins of M. massiliense were distinctly expressed from those of M. abscessus. Bioinformatic analysis of the identified proteins revealed that 3 unique proteins corresponded to serine/arginine rich protein, membrane protein from Streptomyces coelicolor, and one hypothetical protein from Corynebacterium efficiens YS-314, respectively. Culture filtrate proteins from M. massiliense induced the release of pro-inflammatory cytokines from macrophages in a dose-dependent manner but not that from M. abscessus. Taken together, the functional study on the identified proteins uniquely produced from M. massiliense may provide not only the clues for the different pathogensis, but also help develop the diagnostic tools for the differentiation between two mycobacterial species.
Shin
A-Rum
AR
Department of Microbiology and Research Institute for Medical Sciences, College of Medicine, Chungnam National University, Daejeon, Republic of Korea.
Sohn
Hosung
H
Won
Choul Jae
CJ
Lee
Byungsoo
B
Kim
Woo Sik
WS
Kang
Hyun Bae
HB
Kim
Hwa-Jung
HJ
Cho
Sang Nae
SN
Shin
Sung Jae
SJ
D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D016207:Cytokines / Q000276:immunology; D015180:Electrophoresis, Gel, Two-Dimensional; D005110:Extracellular Space / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D005260:Female; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D009161:Mycobacterium / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D009164:Mycobacterium Infections / Q000276:immunology / Q000382:microbiology*; D021241:Spectrometry, Mass, Electrospray Ionization
D001426:Bacterial Proteins; D016207:Cytokines
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