[Norcantharidin inhibits DNA replication initiation protein Cdc6 in cancer cells].
OBJECTIVE
To explore the inhibitory effect of norcantharidin (NCTD) on the expression of DNA replication initiation protein Cdc6 in cancer cells.


METHODS
MTT assay was performed to detect the inhibitory effect on different cancer cell lines, including HeLa, HepG2, Jurkat and Ramos cells. The effect of NCTD on Cdc6 protein level was detected by Western blotting, and BrdU incorporation assay was used to evaluate the DNA replication of the cells.


RESULTS
NCTD significantly inhibited the proliferation of the cells and caused degradation of Cdc6 protein to result in the inhibition of the DNA replication of the cells shown by BrdU incorporation assay.


CONCLUSIONS
NCTD can induce the degradation of Cdc6 in cancer cells to produce an anti-cancer effect.
Li
Jin-long
JL
Institute of Biotherapy, School of Biotechnology, Southern Medical University, Guangzhou 510515, China. lijinlong75@sina.com
Cai
Yu-chen
YC
Hu
Zhi-ming
ZM
Gao
Ji-min
JM
D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000494:pharmacology*; D018797:Cell Cycle Proteins / Q000378:metabolism*; D045744:Cell Line, Tumor; D004261:DNA Replication / Q000187:drug effects*; D006801:Humans; D009687:Nuclear Proteins / Q000378:metabolism*
D019086:Bridged Bicyclo Compounds, Heterocyclic; C103706:CDC6 protein, human; D018797:Cell Cycle Proteins; D009687:Nuclear Proteins; C069741:norcantharidin