Hybrid pseudomonads engineered by two-step homologous recombination acquire novel degradation abilities toward aromatics and polychlorinated biphenyls.
Pseudomonas pseudoalcaligenes KF707 possesses a chromosomally encoded bph gene cluster responsible for the catabolism of biphenyl and polychlorinated biphenyls. Previously, we constructed chimeric versions of the bphA1 gene, which encodes a large subunit of biphenyl dioxygenase, by using DNA shuffling between bphA1 genes from P. pseudoalcaligenes KF707 and Burkholderia xenovorans LB400. In this study, we demonstrate replacement of the bphA1 gene with chimeric bphA1 sequence within the chromosomal bph gene cluster by two-step homologous recombination. Notably, some of the hybrid strains acquired enhanced and/or expanded degradation capabilities for specific aromatic compounds, including single aromatic hydrocarbons and polychlorinated biphenyls.
Suenaga
Hikaru
H
Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba 305-8566, Japan. suenaga-hikaru@aist.go.jp
Nonaka
Kazuhiko
K
Fujihara
Hidehiko
H
Goto
Masatoshi
M
Furukawa
Kensuke
K
D001673:Biodegradation, Environmental; D005818:Genetic Engineering; D005810:Multigene Family; D010105:Oxygenases / Q000378:metabolism*; D011078:Polychlorinated Biphenyls / Q000378:metabolism*; D044205:Pseudomonas pseudoalcaligenes / Q000235:genetics* / Q000378:metabolism; D011995:Recombination, Genetic; D013379:Substrate Specificity
D011078:Polychlorinated Biphenyls; D010105:Oxygenases; C092087:biphenyl-1,2-dioxygenase