Evaluation of MGIT 960 and the colorimetric-based method for tuberculosis drug susceptibility testing.
METHODS
Dr Cetrángolo Hospital, Buenos Aires Province, Argentina.


OBJECTIVE
Evaluation of the BACTEC Mycobacteria Growth Indicator Tube (MGIT) 960 system and the colorimetric-based method (CMM) for first- and second-line drug susceptibility testing (FL-DST, SL-DST) against Mycobacterium tuberculosis.


METHODS
FL-DST was studied using SIRE MGIT 960. Minimal inhibitory concentrations (MICs) for isoniazid (INH), streptomycin, rifampicin (RMP), ethambutol (EMB) and levofloxacin (LVX) were also determined by CMM using 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT). MICs for amikacin (AMK), kanamycin (KM), capreomycin (CPM), ethionamide (ETH), cycloserine, ofloxacin (OFX), linezolide (LZ) and moxifloxacin (MFX) were determined on 94 multidrug-resistant M. tuberculosis isolates by MGIT 960 and CMM. Statistical methods were applied to define drug-susceptible and drug-resistant isolates on the basis of the comparison between results obtained by gold standards.


RESULTS
A total of 1626 clinical isolates were studied. Critical drug concentrations could be defined in less than 10 days for both CMM and MGIT 960. CMM was cheaper but more laborious than MGIT 960. The highest performances of both methods were achieved for AMK, RMP, OFX, LZ and MFX, followed by INH, ETH, KM, CPM and LVX (tested only by CMM).


CONCLUSIONS
Both methods could be implemented as rapid diagnostic tools to detect drug-resistant isolates in clinical practice.
Morcillo
N
N
Buenos Aires Province Tuberculosis Control Program Reference Laboratory, Dr Cetrángolo Hospital, Buenos Aires, Argentina. nora_morcillo@yahoo.com.ar
Imperiale
B
B
Di Giulio
B
B
D000995:Antitubercular Agents / Q000494:pharmacology*; D001118:Argentina; D001431:Bacteriological Techniques; D003124:Colorimetry / Q000379:methods*; D024901:Drug Resistance, Multiple, Bacterial; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods; D009169:Mycobacterium tuberculosis / Q000187:drug effects*; D013997:Time Factors
D000995:Antitubercular Agents