Nucleating the assembly of macromolecular complexes.
Nature constructs intricate complexes containing numerous binding partners in order to direct a variety of cellular processes. Researchers have taken a cue from these events to develop synthetic molecules that can nucleate natural and unnatural interactions for a diverse set of applications. These molecules can be designed to drive protein dimerization or to modulate the interactions between proteins, lipids, DNA, or RNA and thereby alter cellular pathways. A variety of components within the cellular machinery can be recruited with or replaced by synthetic compounds. Directing the formation of multicomponent complexes with new synthetic molecules can allow unprecedented control over the cellular machinery.
Peterson-Kaufman
Kimberly J
KJ
Department of Biochemistry, University of Wisconsin, Madison, WI 53706, USA.
Carlson
Clayton D
CD
Rodríguez-Martínez
José A
JA
Ansari
Aseem Z
AZ
D000818:Animals; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D046912:Multiprotein Complexes / Q000737:chemistry* / Q000378:metabolism; D011487:Protein Conformation; D055550:Protein Stability; D011506:Proteins / Q000737:chemistry / Q000378:metabolism; D012313:RNA / Q000737:chemistry / Q000378:metabolism; D015398:Signal Transduction; D054852:Small Molecule Libraries / Q000737:chemistry* / Q000378:metabolism; D058615:Synthetic Biology / Q000379:methods*
D046912:Multiprotein Complexes; D011506:Proteins; D054852:Small Molecule Libraries; D012313:RNA; D004247:DNA
J Am Chem Soc. 2004 Mar 31;126(12):3748-54
15038727
Science. 1993 Nov 12;262(5136):1019-24
7694365
J Neurosci. 2009 Sep 30;29(39):12079-88
19793966
FEBS Lett. 2009 Jun 5;583(11):1692-8
19303011
Cell. 1991 May 31;65(5):717-9
2040012
Yeast. 2008 Aug;25(8):577-94
18668531
Nat Chem Biol. 2009 Nov;5(11):815-22
19841631
Annu Rev Biochem. 1984;53:389-446
6206781
Physiol Rev. 2003 Jul;83(3):965-1016
12843413
J Am Chem Soc. 2004 Sep 1;126(34):10504-5
15327284
Nature. 2002 Jan 3;415(6867):81-4
11780121
FEBS Lett. 2009 Dec 17;583(24):4006-12
19800332
Curr Opin Chem Biol. 2005 Jun;9(3):317-24
15939335
Chem Biol. 2003 Dec;10(12):1303-11
14700637
Curr Opin Chem Biol. 2008 Aug;12(4):464-71
18706517
Proc Natl Acad Sci U S A. 2002 May 14;99(10):7060-5
11983857
Bioorg Med Chem Lett. 2008 Nov 15;18(22):5904-8
18752944
Cell. 2002 Feb 22;108(4):557-72
11909526
J Biol Chem. 2000 Oct 6;275(40):31318-24
10913130
Nat Rev Drug Discov. 2004 Apr;3(4):301-17
15060526
Cell. 1995 Dec 15;83(6):835-9
8521507
Anal Biochem. 2007 Apr 1;363(1):160-2
17291441
J Am Chem Soc. 2008 Jun 18;130(24):7736-45
18505252
J Am Chem Soc. 2008 Oct 8;130(40):13186-7
18788807
Proc Natl Acad Sci U S A. 2004 Oct 12;101(41):14707-12
15456909
Chembiochem. 2005 Jan;6(1):40-6
15532104
Nat Biotechnol. 2002 Feb;20(2):135-41
11821858
Biochem Soc Trans. 2005 Dec;33(Pt 6):1265-8
16246094
Proc Natl Acad Sci U S A. 1994 Nov 8;91(23):11163-7
7972027
RNA. 2009 Dec;15(12):2083-104
19850913
J Am Chem Soc. 2004 Nov 24;126(46):15051-9
15548001
Science. 1997 Apr 18;276(5311):421-5
9103202
J Biol Chem. 2004 May 14;279(20):21415-20
15039430
Mol Cell. 2008 Sep 26;31(6):925-32
18922474
J Am Chem Soc. 2009 Apr 8;131(13):4774-82
19290630
Annu Rev Genet. 2000;34:77-137
11092823
Science. 2003 Feb 14;299(5609):1061-4
12511654
ACS Chem Biol. 2008 Apr 18;3(4):220-9
18422304
Annu Rev Biophys. 2010;39:515-37
20192780
Chem Biol. 2001 Jun;8(6):583-92
11410377
Curr Opin Biotechnol. 2002 Oct;13(5):459-67
12459338
J Biol Chem. 2010 Apr 9;285(15):11057-60
20147751
Bioorg Med Chem. 1998 Aug;6(8):1293-9
9784870
Curr Opin Struct Biol. 2005 Aug;15(4):441-6
15993577
J Am Chem Soc. 2004 Dec 15;126(49):15940-1
15584709
Curr Opin Chem Biol. 2000 Dec;4(6):696-703
11102876
Nature. 1997 Dec 4;390(6659):477-84
9393998
Comb Chem High Throughput Screen. 2007 Sep;10(8):667-75
18045079
Nature. 1997 Apr 10;386(6625):569-77
9121580
Cell. 1998 Feb 6;92(3):367-80
9476896
J Am Chem Soc. 2003 Nov 5;125(44):13322-3
14583004
Neuron. 2007 Jul 19;55(2):201-15
17640523
Nature. 1989 Jul 20;340(6230):245-6
2547163
Nat Chem Biol. 2005 Jul;1(2):64-6
16407997
Science. 1997 Jan 31;275(5300):657-61
9005850
Chem Biol. 2006 Jan;13(1):11-21
16426967
Proc Natl Acad Sci U S A. 2003 Feb 18;100(4):1603-8
12582206
Chem Biol. 1996 Sep;3(9):731-8
8939689
J Biol Chem. 2010 Jan 22;285(4):2498-505
19889640
Nature. 2004 Mar 25;428(6981):437-41
15042093
Chem Biol. 2005 Feb;12(2):151-61
15734643
ACS Chem Biol. 2008 Nov 21;3(11):677-692
19112665
Microbiol Rev. 1995 Mar;59(1):94-123
7708014
Cell. 1988 Nov 4;55(3):443-6
3180218
Proc Natl Acad Sci U S A. 1996 Aug 6;93(16):8496-501
8710898
Trends Biochem Sci. 1999 Jan;24(1):8-11
10087914
Trends Biochem Sci. 1996 Nov;21(11):418-22
8987395
Nat Methods. 2009 Dec;6(12):917-22
19915560
Nature. 1996 Sep 12;383(6596):178-81
8774884
Biopolymers. 2006;84(6):535-52
17009316
Arch Biochem Biophys. 2006 Aug 1;452(1):55-68
16806044
Proc Natl Acad Sci U S A. 1996 Nov 12;93(23):12817-21
8917502
Biochem Soc Trans. 2010 Feb;38(Pt 1):29-33
20074030
ACS Chem Biol. 2009 May 15;4(5):335-44
19348463
J Am Chem Soc. 2007 Sep 5;129(35):10654-5
17691790
Proc Natl Acad Sci U S A. 1996 May 14;93(10):4604-7
8643450
Angew Chem Int Ed Engl. 2007;46(16):2865-8
17351994
Science. 2008 Mar 14;319(5869):1539-43
18339942
Angew Chem Int Ed Engl. 2006 Apr 3;45(15):2348-68
16557636
Angew Chem Int Ed Engl. 1998 Nov 2;37(20):2754-2794
29711117
Proc Natl Acad Sci U S A. 2005 Apr 5;102(14):5008-13
15781856
Angew Chem Int Ed Engl. 2001 Mar 2;40(5):871-875
29712174
Biochim Biophys Acta. 2008 Nov;1780(11):1325-36
18206122
Curr Opin Chem Biol. 2000 Feb;4(1):60-3
10679376
J Am Chem Soc. 2007 Mar 7;129(9):2660-8
17290996
Proc Natl Acad Sci U S A. 2000 Apr 11;97(8):3930-5
10760265
Chem Rev. 2004 Feb;104(2):789-99
14871141
Proc Natl Acad Sci U S A. 2002 Dec 24;99(26):16537-42
12482929
Proc Natl Acad Sci U S A. 1998 Dec 8;95(25):14628-33
9843940
Annu Rev Biochem. 1979;48:719-54
382996
Trends Biochem Sci. 1988 Nov;13(11):443-7
3075366
Mol Cell. 2009 Dec 25;36(6):924-31
20064459