title: A mecA-negative strain of methicillin-resistant Staphylococcus aureus with high-level β-lactam resistance contains mutations in three genes.
abstract: We previously generated a ceftobiprole-resistant Staphylococcus aureus strain after high inoculum serial passage of a mecA-negative variant of strain COL (R. Banerjee, M. Gretes, L. Basuino, N. Strynadka, and H. F. Chambers, Antimicrob. Agents Chemother. 52:2089-2096, 2008). Genome resequencing of this strain, CRB, revealed that it differs from its parent by five single-nucleotide polymorphisms in three genes, specifically, those encoding PBP4, a low-molecular-weight penicillin-binding protein, GdpP, a predicted signaling protein, and AcrB, a cation multidrug efflux transporter. CRB displayed resistance to a variety of β-lactams but was hypersusceptible to cefoxitin.
authors.affiliation: Division of Pediatric Infectious Diseases, Mayo Clinic, 200 First St. SW, Rochester, MN 55905, USA. Banerjee.ritu@mayo.edu
authors.forename: Ritu
authors.initials: R
authors.lastname: Banerjee
authors.forename: Michael
authors.initials: M
authors.lastname: Gretes
authors.forename: Christopher
authors.initials: C
authors.lastname: Harlem
authors.forename: Li
authors.initials: L
authors.lastname: Basuino
authors.forename: Henry F
authors.initials: HF
authors.lastname: Chambers
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D009154:Mutation; D046915:Penicillin-Binding Proteins / Q000235:genetics; D018440:beta-Lactam Resistance / Q000235:genetics*; D047090:beta-Lactams / Q000494:pharmacology
chemical_list: D001426:Bacterial Proteins; D046915:Penicillin-Binding Proteins; D047090:beta-Lactams; C487789:mecA protein, Staphylococcus aureus
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