[Effect of polyethylene glycol as adjuvant on hepatitis B virus DNA vaccine in vitro].
OBJECTIVE
To explore whether polyethylene glycol (PEG) as adjuvant could enhance the humoral and cellular immune responses on hepatitis B virus DNA vaccine.


METHODS
C57BL/6J mice were immunized with PEG and pVAX-S2 or alone pVAX-S2. After these mice were finally immunized for 14 days, the anti-HBs IgG, T cell proliferation, the expression of cytokines and CTL in vivo were detected.


RESULTS
Compared to mice immunized with alone pVAX-S2, PEG as adjuvant could increase the production of anti-HBs IgG and HBsAg specific T cell proliferation. In addition, the expression of IL-4, IFN-gamma in CD4+ T cells and IFN-gamma in CD8+ T cells was higher than control groups. The PEG/ pVAX-S2 groups could elicit significantly in vivo HBsAg specific CTL responses.


CONCLUSIONS
PEG as adjuvant could enhance humoral and cellular immune responses, as well as in vivo CTL activity.
Wang
Junpeng
J
Key Laboratory of Cellular and Molecular Immunology of College of Medicine; 2Institute of Immunology, Henan University, Kaifeng 475001, China. jpwangchina@henu.edu.cn
Lu
Ligan
L
Song
Hongli
H
Yang
Yongjie
Y
Ma
Yuanfang
Y
D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000818:Animals; D005260:Female; D006509:Hepatitis B / Q000276:immunology* / Q000517:prevention & control / Q000821:virology; D006510:Hepatitis B Antibodies / Q000097:blood / Q000276:immunology; D017325:Hepatitis B Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D006515:Hepatitis B virus / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007114:Immunization; D051379:Mice; D008810:Mice, Inbred C57BL; D011092:Polyethylene Glycols / Q000008:administration & dosage*; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology
D000276:Adjuvants, Immunologic; D006510:Hepatitis B Antibodies; D017325:Hepatitis B Vaccines; D019444:Vaccines, DNA; D011092:Polyethylene Glycols