Analysis of MAP kinases by hydrogen exchange mass spectrometry.
Hydrogen exchange mass spectrometry (HX-MS) is an experimental technique that can be used to -examine solvent accessibility and conformational mobility in biological macromolecules. This chapter summarizes studies using HX-MS to examine the regulation of conformation, protein mobility, and ligand binding to MAP kinases. We describe the planning and design of HX-MS experiments, strategies for data analysis and interpretation, and available software.
Sours
Kevin M
KM
Department of Chemistry and Biochemistry, University of Colorado, Boulder, CO, USA.
Ahn
Natalie G
NG
D016477:Artifacts; D041961:Deuterium Exchange Measurement / Q000379:methods*; D004789:Enzyme Activation; D057075:Enzyme Assays / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D012984:Software
D019950:Mitogen-Activated Protein Kinase 1; D020928:Mitogen-Activated Protein Kinases
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