title: Comparative analysis of FcεRI expression patterns in patients with eosinophilic and reflux esophagitis.
abstract: OBJECTIVE
  Eosinophilic esophagitis (EoE) is an allergic disease of the esophagus. The IgE receptors on immune cells that infiltrate the esophagus are poorly defined. The high-affinity receptor for IgE, FcεRI, may play a role in EoE. The objective of the present study is to identify and compare the IgE receptors in the esophageal epithelium of patients with EoE, reflux esophagitis (RE), and normal controls.
  
  
  METHODS
  A retrospective case-control study of 62 patients (19 EoE, 22 RE, 21 normal controls) was conducted. Biopsies were immunostained for FcεRI, CD23, galectin-3, c-kit, CD1a, and langerin.
  
  
  RESULTS
  FcεRI was the only IgE receptor present in the esophageal epithelium of patients with EoE. The FcεRI-positive cell count varied by diagnosis (proximal biopsies EoE 32.6 ± 19.0 cells/high-power field, RE 26.7 ± 16.6, controls 15.6 ± 8.3, ANOVA P = 0.005; distal biopsies EoE 24.2 ± 16.2, RE 35.7 ± 27.6, controls 15.3 ± 8.4, P = 0.006). In the proximal esophagus, the FcεRI count was higher in EoE than in controls (P = 0.006); in the distal esophagus, the FcεRI count was higher in RE than in controls (P = 0.004). EoE and RE had similar FcεRI-positive cell counts. A subset of FcεRI-positive cells was similar in morphology and distribution to Langerhans cells (CD1a and langerin positive).
  
  
  CONCLUSIONS
  The presence of FcεRI-positive cells in high numbers in the esophageal epithelium implies this receptor must be critical in the IgE-mediated activation of immune cells in the esophagus. Langerhans cells in the esophageal epithelium appear to express FcεRI. The role of Langerhans cells in the pathophysiology of EoE needs to be elucidated.
authors.affiliation: Division of Gastroenterology, Children's Hospital Boston, Boston, MA 02115, USA.
authors.forename: Elizabeth H
authors.initials: EH
authors.lastname: Yen
authors.forename: Jason L
authors.initials: JL
authors.lastname: Hornick
authors.forename: Eleonora
authors.initials: E
authors.lastname: Dehlink
authors.forename: Maarten
authors.initials: M
authors.lastname: Dokter
authors.forename: Alexandra
authors.initials: A
authors.lastname: Baker
authors.forename: Edda
authors.initials: E
authors.lastname: Fiebiger
authors.forename: Samuel
authors.initials: S
authors.lastname: Nurko
mesh_terms: D016022:Case-Control Studies; D002452:Cell Count; D002648:Child; D057765:Eosinophilic Esophagitis / Q000276:immunology*; D004804:Eosinophils / Q000378:metabolism; D004942:Esophagitis, Peptic / Q000276:immunology*; D004947:Esophagus / Q000276:immunology*; D005260:Female; D006801:Humans; D007073:Immunoglobulin E / Q000378:metabolism*; D007801:Langerhans Cells / Q000378:metabolism*; D008297:Male; D009092:Mucous Membrane / Q000276:immunology*; D011961:Receptors, Fc / Q000378:metabolism*; D012189:Retrospective Studies
chemical_list: D011961:Receptors, Fc; D007073:Immunoglobulin E
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