title: Effect of milrinone analogues on intracellular calcium increase in single living H9C2 cardiac cells.
abstract: The synthesis of milrinone analogues where the 4-pyridyl moiety was replaced by an ester or amide group is reported. Only amide derivatives are able to support intracellular calcium influx following chemical depolarization with 60 mM KCl in a percentage varying from 20 to 45% of differentiated H9C2 cardiomyocytes. Those cells were differentiated after chronic exposure to 10 nM retinoic acid which induces the expression of voltage-gated calcium channels. Analogues of milrinone containing an ester function did not show significant activity.
authors.affiliation: Dipartimento di Scienze Mediche di Base e Applicate, Università degli Studi G. d'Annunzio, via dei Vestini 31, 66100 Chieti, Italy.
authors.forename: Tiziana
authors.initials: T
authors.lastname: Pietrangelo
authors.forename: Letizia
authors.initials: L
authors.lastname: Giampietro
authors.forename: Barbara
authors.initials: B
authors.lastname: De Filippis
authors.forename: Rita
authors.initials: R
authors.lastname: La Rovere
authors.forename: Stefania
authors.initials: S
authors.lastname: Fulle
authors.forename: Rosa
authors.initials: R
authors.lastname: Amoroso
mesh_terms: D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000378:metabolism; D002316:Cardiotonic Agents / Q000494:pharmacology* / Q000633:toxicity; D002460:Cell Line; D006321:Heart / Q000187:drug effects*; D006801:Humans; D015640:Ion Channel Gating; D020105:Milrinone / Q000494:pharmacology* / Q000633:toxicity; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*
chemical_list: D015220:Calcium Channels; D002316:Cardiotonic Agents; D020105:Milrinone; D002118:Calcium