Optimization of culture conditions for mycoepoxydiene production by Phomopsis sp. Hant25.
Culture media and fermentation conditions for cultivation of an endophytic fungus Phomopsis sp. Hant25 were investigated in order to improve the yield of mycoepoxydiene, a novel fungal metabolite having potent cytotoxic activity against many cancer cell lines. Mycoepoxydiene accumulated in the culture broth during the stationary phase of fungal growth. Modified M1D medium was superior to malt Czapek, and Czapek yeast autolysate broths in supporting mycoepoxydiene production. Pellet growth was the morphological form that favored biosynthesis of mycoepoxydiene. This could be achieved by incubating the culture statically for 6 days before shaking at 120 rpm. Incorporation of a cellulose paper disc into the culture flask promoted fungal growth at the liquid surface, which accelerated mycoepoxydiene production and maximized the final yield to a level of 354 mg l⁻¹, though fungal attachment to the solid support was not required. Since the peak concentration of mycoepoxydiene in the culture broth was followed by a steeply declining phase, the harvest time had to be precisely determined for maximum product yield. Understanding the factor(s) involved in rapid degradation of mycoepoxydiene could lead to improved final yields.
Thammajaruk
Narukjaporn
N
Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand.
Sriubolmas
Nongluksna
N
Israngkul
Duangnate
D
Meevootisom
Vithaya
V
Wiyakrutta
Suthep
S
D001203:Ascomycota / Q000378:metabolism*; D001952:Bridged-Ring Compounds / Q000737:chemistry / Q000378:metabolism*; D002482:Cellulose / Q000737:chemistry; D003470:Culture Media; D005285:Fermentation; D011753:Pyrones / Q000737:chemistry / Q000378:metabolism*
D001952:Bridged-Ring Compounds; D003470:Culture Media; D011753:Pyrones; C476085:mycoepoxydiene; D002482:Cellulose
Proc Natl Acad Sci U S A. 1976 Nov;73(11):4007-11
16592362
Microbiology (Reading). 1996 Feb;142 ( Pt 2):435-440
8932715
Org Lett. 2002 Aug 22;4(17):2941-3
12182594
Planta Med. 2007 Oct;73(13):1418-20
17926268
Phytochemistry. 2009 Jan;70(1):121-7
19038408
J Ind Microbiol Biotechnol. 2006 Oct;33(10):815-26
16680458
J Ind Microbiol Biotechnol. 2010 Apr;37(4):335-40
20033470
J Org Chem. 2004 Dec 10;69(25):8789-95
15575759
FEMS Microbiol Lett. 2005 Oct 1;251(1):53-8
16102912
Biotechnol Adv. 2004 Jan;22(3):189-259
14665401
Annu Rev Phytopathol. 2005;43:437-58
16078891