In vivo study of photosensitizer pharmacokinetics by fluorescence transillumination imaging.
The possibility of in vivo investigation of the pharmacokinetics of photosensitizers by means of fluorescence transillumination imaging is demonstrated. An animal is scanned in the transilluminative configuration by a single source and detector pair. Transillumination is chosen as an alternative approach to reflection imaging. In comparison with the traditional back-reflection technique, transillumination is preferable for photosensitizer detection due to its higher sensitivity to deep-seated fluorophores. The experiments are performed on transplantable mouse cervical carcinomas using three drugs: photosens, alasens, and fotoditazin. For quantitative evaluation of the photosensitizer concentration in tumor tissue the fluorescence signal is calibrated using tissue phantoms. We show that the kinetics of photosensitizer tumor uptake obtained by transillumination imaging in vivo agree with data of standard ex vivo methods. The described approach enables rapid and cost-effective study of newly developed photosensitizers in small animals.
Shirmanova
Marina
M
Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russia. shirmanovam@mail.ru
Zagaynova
Elena
E
Sirotkina
Marina
M
Snopova
Ludmila
L
Balalaeva
Irina
I
Krutova
Irina
I
Lekanova
Nataliya
N
Turchin
Ilya
I
Orlova
Anna
A
Kleshnin
Michail
M
D000818:Animals; D045744:Cell Line, Tumor; D005240:Feasibility Studies; D005260:Female; D008657:Metabolic Clearance Rate; D051379:Mice; D017319:Photosensitizing Agents / Q000493:pharmacokinetics*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D014018:Tissue Distribution; D002583:Uterine Cervical Neoplasms / Q000188:drug therapy* / Q000378:metabolism*
D017319:Photosensitizing Agents