title: Tumour visualization in a murine model by time-delayed fluorescence of sulphonated aluminium phthalocyanine.
abstract: Mice bearing the MS-2 fibrosarcoma were administered 0.25, 0.5 or 1 mg kg(-1) body weight (b.w.) of sulphonated aluminium phthalocyanine (AlS(2)Pc) (with average degree of sulphonation of 2.1), and time-gated fluorescence images were acquired up to 6 h after the injection. Different excitation wavelengths (610, 650 and 670 nm) were tested. Red light excitation and 3 ns delayed detection allow one to minimize natural fluorescence and scattered laser light, respectively. The best conditions for tumour detection are reached under either 650 or 670 nm Excitation, 2-4 h after the administration of either 0.5 or 1 mg kg(-1) b.w. of AlS(2)Pc. In these situations, the average fluorescence contrast between tumour area and surrounding healthy tissue is > 2, providing a clear identification of the pathological region. However, tumour localization is possible even after the injection of 0.25 mg kg(-1) b.w. of sensitizer. In conclusion, under low power excitation (< 100MuW cm(-2)), the technique allows real time detection of an intradermal tumour with good contrast.
authors.affiliation: C.E.Q.S.E.-C.N.R., Politecnico di Milano, Piazza L. da Vinci 32, 20133, Milano, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Cubeddu
authors.forename: G
authors.initials: G
authors.lastname: Canti
authors.forename: P
authors.initials: P
authors.lastname: Taroni
authors.forename: G
authors.initials: G
authors.lastname: Valentini
references.citation: Appl Opt. 1994 Dec 1;33(34):8022-9
references.pmid: 20963019
references.citation: Photochem Photobiol. 1993 Mar;57(3):480-5
references.pmid: 8475182
references.citation: Photochem Photobiol. 1987 Nov;46(5):887-91
references.pmid: 2964668
references.citation: J Photochem Photobiol B. 1996 Jul;34(2-3):229-35
references.pmid: 8810541
references.citation: Lasers Surg Med. 1984;4(1):49-58
references.pmid: 6235415
references.citation: J Photochem Photobiol B. 1995 Aug;29(2-3):171-8
references.pmid: 7472811
references.citation: J Photochem Photobiol B. 1991 Dec;11(3-4):319-28
references.pmid: 1816367
references.citation: Photochem Photobiol. 1992 Jan;55(1):145-57
references.pmid: 1603846
references.citation: J Photochem Photobiol B. 1990 Jun;6(1-2):111-9
references.pmid: 2121928
references.citation: Photochem Photobiol. 1993 Jun;57(6):978-83
references.pmid: 8367537
references.citation: Photochem Photobiol. 1987 Nov;46(5):759-63
references.pmid: 2964662
references.citation: IEEE Trans Biomed Eng. 1989 Dec;36(12):1146-54
references.pmid: 2606488
references.citation: Photochem Photobiol. 1987 Jun;45(6):879-89
references.pmid: 2957705
references.citation: Photochem Photobiol. 1986 Jun;43(6):691-9
references.pmid: 3092251
references.citation: Lasers Surg Med. 1994;15(4):351-7
references.pmid: 7885168
references.citation: Photochem Photobiol. 1991 Jun;53(6):859-70
references.pmid: 1886943
references.citation: Med Phys. 1987 Jul-Aug;14(4):637-9
references.pmid: 2957581
references.citation: J Photochem Photobiol B. 1994 Feb;22(2):105-17
references.pmid: 8176544