Cholesterol intake modulates plasma triglyceride levels in glycosylphosphatidylinositol HDL-binding protein 1-deficient mice.
OBJECTIVE
To determine whether plasma triglyceride levels in adult Glycosylphosphatidylinositol HDL-binding protein 1 (GPIHBP1)-deficient (Gpihbp1(-/-)) mice would be sensitive to cholesterol intake.


RESULTS
Gpihbp1(-/-) mice were fed a Western diet containing 0.15% cholesterol. After 4 to 8 weeks, their plasma triglyceride levels were 113 to 135 mmol/L. When 0.005% ezetimibe was added to the diet to block cholesterol absorption, Lpl expression in the liver was reduced significantly, and the plasma triglyceride levels were significantly higher (>170 mmol/L). We also assessed plasma triglyceride levels in Gpihbp1(-/-) mice fed Western diets containing either high (1.3%) or low (0.05%) amounts of cholesterol. The high-cholesterol diet significantly increased Lpl expression in the liver and lowered plasma triglyceride levels.


CONCLUSIONS
Treatment of Gpihbp1(-/-) mice with ezetimibe lowers Lpl expression in the liver and increases plasma triglyceride levels. A high-cholesterol diet had the opposite effects. Thus, cholesterol intake modulates plasma triglyceride levels in Gpihbp1(-/-) mice.
Weinstein
Michael M
MM
Department of Medicine, David Geffen School of Medicine, University of California, Los Angeles, USA.
Tu
Yiping
Y
Beigneux
Anne P
AP
Davies
Brandon S J
BS
Gin
Peter
P
Voss
Constance
C
Walzem
Rosemary L
RL
Reue
Karen
K
Tontonoz
Peter
P
Bensadoun
André
A
Fong
Loren G
LG
Young
Stephen G
SG
D000818:Animals; D000924:Anticholesteremic Agents / Q000494:pharmacology*; D001384:Azetidines / Q000494:pharmacology*; D002784:Cholesterol / Q000378:metabolism*; D004041:Dietary Fats / Q000378:metabolism*; D004195:Disease Models, Animal; D000069438:Ezetimibe; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D018110:Receptors, Lipoprotein / Q000172:deficiency*; D014280:Triglycerides / Q000097:blood / Q000378:metabolism*
D000924:Anticholesteremic Agents; D001384:Azetidines; D004041:Dietary Fats; C472779:GPI-HBP1 protein, mouse; D018110:Receptors, Lipoprotein; D014280:Triglycerides; D002784:Cholesterol; D000069438:Ezetimibe
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