[Everted intestinal sac method for quick finding absorption ingredients of Wuzhuyu decoction].
OBJECTIVE
To establish a method for quick finding the absorption ingredients of Wuzhuyu decoction in order to select the index to control its quality.


METHODS
The absorption of three concentration of Wuzhuyu decotion was investigated with the in vitro-everted intestinal sac model. The intestinal bag fluid of jejunum and ileum were collected in different time and the eight ingredients, which were evodiamine (Ev), rutaecarpine (Ru), limonin (Li), ginsenoside-Rb1, -Rg1, -Re (Rb1, Rg1, Re), isorhamnetin-3-O-beta-D-glucosyl(6''-->1'")-alpha-L-rhamnoside (Irs)and 6-gingerol (6-Gi), were detected by HPLC as the represent constituents in samples.


RESULTS
Eight ingredients except Ru in samples could be detected, but Ev could not be detected in high concentration samples. The ratios between absorption ingredients were different from in Wuzhuyu decotion.


CONCLUSIONS
The in vitro-everted intestinal sac canc absorb the ingredients of Wuzhuyu decotion selectivity. Compare with the ileum, the jejunum can provide the more absorption information and faster, the best test time is 60-90 min.
Gong
Muxin
M
School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China.
Wang
Yaxun
Y
Song
Yafang
Y
Wang
Zhimin
Z
Zhang
Qiwei
Q
Wang
Weihao
W
Zhu
Jingjing
J
D000818:Animals; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000493:pharmacokinetics*; D045604:Extracellular Fluid / Q000737:chemistry* / Q000187:drug effects; D007082:Ileum / Q000737:chemistry / Q000187:drug effects; D007408:Intestinal Absorption; D007583:Jejunum / Q000737:chemistry / Q000187:drug effects; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D004365:Drugs, Chinese Herbal