Recording electric potentials from single adherent cells with 3D microelectrode arrays after local electroporation.
This short communication reports on the innovative method of the local micro-invasive needle electroporation (LOMINE) of single adherent cells. The investigation of cellular reactions in living cell cultures represents a fundamental method, e.g. for drug development and environmental monitoring. Existing classical methods for intracellular measurements using, e.g. patch clamp techniques are time-consuming and complex. Present patch-on-chip systems are limited to the investigation of single cells in suspension. Nevertheless, the most part of the cells of the human body is adherently growing. Therefore, we develop a new chip system for the growth of adherent cells with 64 micro-structured needle electrodes as well as 128 dielectrophoretic electrodes, located within a measuring area of 1 mm(2). With this analytical chip, the intracellular investigation of electro-chemical changes and processes in adherently growing cells will become possible in the near future. Here, we present first intracellular measurements with this chip system.
Koester
Philipp Julian
PJ
University of Rostock, Chair of Biophysics, Gertrudenstrasse 11a, 18057 Rostock, Germany. philipp.koester@uni-rostock.de
Tautorat
Carsten
C
Beikirch
Helmut
H
Gimsa
Jan
J
Baumann
Werner
W
D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D018274:Electroporation; D006801:Humans; D056656:Lab-On-A-Chip Devices; D008564:Membrane Potentials; D051379:Mice; D008839:Microelectrodes; D008855:Microscopy, Electron, Scanning; D018408:Patch-Clamp Techniques