Early diagnosis of Dengue infection using a commercial Dengue Duo rapid test kit for the detection of NS1, IGM, and IGG.
A commercial Dengue Duo rapid test kit was evaluated for early dengue diagnosis by detection of dengue virus NS1 antigen and immunoglobulin M (IgM)/IgG antibodies. A total of 420 patient serum samples were subjected to real-time reverse transcription-polymerase chain reaction (RT-PCR), in-house IgM capture enzyme-linked immunosorbent assay (ELISA), hemagglutination inhibition assay, and the SD Dengue Duo rapid test. Of the 320 dengue acute and convalescent sera, dengue infection was detected by either serology or RT-PCR in 300 samples (93.75%), as compared with 289 samples (90.31%) in the combined SD Duo NS1/IgM. The NS1 detection rate is inversely proportional, whereas the IgM detection rate is directly proportional to the presence of IgG antibodies. The sensitivity and specificity in diagnosing acute dengue infection in the SD Duo NS1/IgM were 88.65% and 98.75%, respectively. The assay is sensitive and highly specific. Detection of both NS1 and IgM by SD Duo gave comparable detection rate by either serology or RT-PCR.
Wang
Seok Mui
SM
Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia. seokmuiwang@yahoo.com
Sekaran
Shamala Devi
SD
D003715:Dengue / Q000175:diagnosis*; D042241:Early Diagnosis; D004797:Enzyme-Linked Immunosorbent Assay; D006386:Hemagglutination Tests; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D011933:Reagent Kits, Diagnostic; D020133:Reverse Transcriptase Polymerase Chain Reaction
D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011933:Reagent Kits, Diagnostic
PLoS Negl Trop Dis. 2008 Aug 20;2(8):e280
18714359
Am J Trop Med Hyg. 1977 May;26(3):533-7
326071
J Virol Methods. 2007 Mar;140(1-2):75-9
17140671
Am J Trop Med Hyg. 1984 Jan;33(1):158-65
6364855
Adv Virus Res. 1999;53:35-70
10582094
Diagn Microbiol Infect Dis. 2008 Jan;60(1):43-9
17889487
Am J Trop Med Hyg. 1958 Sep;7(5):561-73
13571577
Clin Diagn Lab Immunol. 2004 Jul;11(4):642-50
15242935
Diagn Microbiol Infect Dis. 2009 May;64(1):31-6
19232865
JAMA. 2000 Dec 20;284(23):3043-5
11122593
Southeast Asian J Trop Med Public Health. 2001 Sep;32(3):472-5
11944701
J Clin Microbiol. 2000 Mar;38(3):1053-7
10698995
Diagn Microbiol Infect Dis. 2008 Apr;60(4):387-91
18191361
Clin Vaccine Immunol. 2006 Nov;13(11):1185-9
16988003
Singapore Med J. 2007 Jul;48(7):662-8
17609830
J Clin Microbiol. 2002 Feb;40(2):376-81
11825945
Southeast Asian J Trop Med Public Health. 1987 Dec;18(4):532-8
3329413