Handheld array-based photoacoustic probe for guiding needle biopsy of sentinel lymph nodes.
By modifying a clinical ultrasound array system, we develop a novel handheld photoacoustic probe for image-guided needle biopsy. The integration of optical fiber bundles for pulsed laser light delivery enables photoacoustic image-guided insertion of a needle into rat axillary lymph nodes with accumulated indocyanine green (ICG). Strong photoacoustic contrast of the needle is achieved. After subcutaneous injection of the dye in the left forepaw, sentinel lymph nodes are easily detected, in vivo and in real time, beneath 2-cm-thick chicken breast overlaying the axillary region. ICG uptake in axillary lymph nodes is confirmed with fluorescence imaging both in vivo and ex vivo. These results demonstrate the clinical potential of this handheld photoacoustic system for facile identification and needle biopsy of sentinel lymph nodes for cancer staging and metastasis detection in humans.
Kim
Chulhong
C
Washington University in St. Louis, Department of Biomedical Engineering, St. Louis, Missouri 63130-4899, USA.
Erpelding
Todd N
TN
Maslov
Konstantin
K
Jankovic
Ladislav
L
Akers
Walter J
WJ
Song
Liang
L
Achilefu
Samuel
S
Margenthaler
Julie A
JA
Pashley
Michael D
MD
Wang
Lihong V
LV
D000818:Animals; D001707:Biopsy, Needle / Q000295:instrumentation*; D002645:Chickens; D054459:Elasticity Imaging Techniques / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D008198:Lymph Nodes / Q000000981:diagnostic imaging* / Q000473:pathology*; D008904:Miniaturization; D021701:Sentinel Lymph Node Biopsy / Q000295:instrumentation*; D014159:Transducers; D018084:Ultrasonography, Interventional / Q000295:instrumentation*
J Biomed Opt. 2010 Mar-Apr;15(2):021305
20459227
Chem Rev. 2010 May 12;110(5):2756-82
20210338
Radiology. 1987 Feb;162(2):409-14
3541029
Clin Radiol. 2000 Apr;55(4):281-7
10767187
Eur Urol. 2002 May;41(5):508-14; discussion 514
12074792
Radiology. 2006 Feb;238(2):497-504
16436814
Proc Natl Acad Sci U S A. 2007 Jul 17;104(29):12169-74
17616584
Radiology. 2010 May;255(2):442-50
20413757
J Biomed Opt. 2008 Sep-Oct;13(5):054033
19021413
Nano Lett. 2009 Jan;9(1):183-8
19072058
Radiology. 2010 Jul;256(1):102-10
20574088