title: Apatite microtopographies instruct signaling tapestries for progenitor-driven new attachment of teeth.
abstract: Dimension and structure of extracellular matrix surfaces have powerful influences on cell shape, adhesion, and gene expression. Here we show that natural tooth root topographies induce integrin-mediated extracellular matrix signaling cascades in tandem with cell elongation and polarization to generate physiological periodontium-like tissues. In this study we replanted surface topography instructed periodontal progenitors into rat alveolar bone sockets for 8 and 16 weeks, resulting in complete reattachment of tooth roots to the surrounding alveolar bone with a periodontal fiber apparatus closely matching physiological controls along the entire root surface. Displacement studies and biochemical analyses confirmed that progenitor-based engineered periodontal tissues were similar to control teeth and uniquely derived from preimplantation green fluorescent protein (GFP)-labeled progenitors. Together, these studies illustrate the capacity of natural extracellular surface topographies to instruct progenitor cell populations to fully regenerate complex cellular and structural morphologies of tissues once lost to disease. We suggest that our strategy could be used for the replantation of teeth lost due to trauma or as a novel approach for tooth replacement using tooth-shaped replicas.
authors.affiliation: Brodie Laboratory for Craniofacial Genetics, University of Illinois at Chicago, Chicago, Illinois, USA.
authors.forename: Smit J
authors.initials: SJ
authors.lastname: Dangaria
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Ito
authors.forename: LeiLei
authors.initials: L
authors.lastname: Yin
authors.forename: Giovanni
authors.initials: G
authors.lastname: Valdré
authors.forename: Xianghong
authors.initials: X
authors.lastname: Luan
authors.forename: Thomas G H
authors.initials: TG
authors.lastname: Diekwisch
mesh_terms: D016475:3T3 Cells; D000818:Animals; D002448:Cell Adhesion / Q000502:physiology; D015921:Dental Implants; D003764:Dental Materials / Q000138:chemical synthesis*; D017886:Durapatite / Q000737:chemistry*; D016557:Guided Tissue Regeneration, Periodontal / Q000295:instrumentation*; D051379:Mice; D016062:Porosity; D033581:Stem Cell Transplantation / Q000295:instrumentation* / Q000379:methods; D013499:Surface Properties; D014070:Tooth / Q000166:cytology / Q000254:growth & development*
chemical_list: D015921:Dental Implants; D003764:Dental Materials; D017886:Durapatite
references.citation: Science. 2005 Nov 18;310(5751):1139-43
references.pmid: 16293750
references.citation: Proc Natl Acad Sci U S A. 2009 Aug 11;106(32):13475-80
references.pmid: 19666587
references.citation: J Cell Biol. 2002 Dec 23;159(6):1071-86
references.pmid: 12486108
references.citation: Dent Mater. 2009 Jul;25(7):877-83
references.pmid: 19217655
references.citation: J Periodontal Res. 2008 Dec;43(6):681-8
references.pmid: 18624947
references.citation: Cell. 1990 Mar 9;60(5):849-59
references.pmid: 2155708
references.citation: Acta Biomater. 2010 Apr;6(4):1649-60
references.pmid: 19861182
references.citation: J Histochem Cytochem. 2007 Feb;55(2):127-40
references.pmid: 17015623
references.citation: Brain Res Brain Res Protoc. 2004 Feb;12(3):180-5
references.pmid: 15013469
references.citation: Biomaterials. 2007 Apr;28(10):1787-97
references.pmid: 17218005
references.citation: Eur J Orthod. 1990 Feb;12(1):77-80
references.pmid: 2180729
references.citation: J Orthod. 2001 Dec;28(4):281-90
references.pmid: 11709593
references.citation: Stem Cells Dev. 2006 Aug;15(4):595-608
references.pmid: 16978062
references.citation: Biochem Biophys Res Commun. 2008 Mar 28;368(1):62-7
references.pmid: 18198129
references.citation: J Periodontal Res. 2002 Oct;37(5):317-23
references.pmid: 12366853
references.citation: Biotechnol Prog. 1999 Jan-Feb;15(1):19-32
references.pmid: 9933510
references.citation: J Dent Res. 2000 Sep;79(9):1712-6
references.pmid: 11023268
references.citation: J Periodontal Res. 1999 Apr;34(3):154-9
references.pmid: 10384403
references.citation: Nanomedicine. 2010 Oct;6(5):619-33
references.pmid: 20138244
references.citation: Science. 1999 Aug 13;285(5430):1028-32
references.pmid: 10446041
references.citation: Nat Rev Mol Cell Biol. 2009 Jan;10(1):21-33
references.pmid: 19197329
references.citation: J Clin Periodontol. 2008 Dec;35(12):1066-72
references.pmid: 19040584
references.citation: Science. 2009 Nov 27;326(5957):1216-9
references.pmid: 19965464
references.citation: J Biomed Mater Res. 1991 Jul;25(7):889-902
references.pmid: 1918105
references.citation: J Clin Periodontol. 1988 Jan;15(1):73-80
references.pmid: 3422248
references.citation: J Periodontol. 1995 Jul;66(7):579-93
references.pmid: 7562350
references.citation: Ann Biomed Eng. 2006 Jan;34(1):89-101
references.pmid: 16525765
references.citation: Calcif Tissue Int. 2002 Dec;71(6):519-29
references.pmid: 12232675
references.citation: Mol Cell Biol. 2005 Dec;25(24):11131-44
references.pmid: 16314533
references.citation: Tissue Eng. 2003 Apr;9(2):255-67
references.pmid: 12740088
references.citation: J R Soc Interface. 2008 Oct 6;5(27):1231-42
references.pmid: 18348958
references.citation: PLoS One. 2006 Dec 20;1:e79
references.pmid: 17183711
references.citation: J Orthop Res. 2006 Apr;24(4):619-27
references.pmid: 16514643
references.citation: Nat Cell Biol. 2005 Apr;7(4):336-7
references.pmid: 15803131
references.citation: Nat Cell Biol. 2005 Apr;7(4):343-52
references.pmid: 15793570
references.citation: Regen Med. 2009 Sep;4(5):747-58
references.pmid: 19761399