Biological monitoring of exposure to di(2-ethylhexyl) phthalate in six French factories: a field study.
OBJECTIVE
The aim of this study was to assess, by biological monitoring, workers' exposure to di(2-ethylhexyl) phthalate (DEHP) in the flexible-PVC industry in France to provide additional occupational exposure data, which are particularly scarce.


METHODS
Over 5 days of pre-and post-shift sampling, three urinary metabolites of DEHP, mono (2-ethylhexyl) phthalate (MEHP), mono (5-carboxy-2-ethylpentyl) phthalate (5cx-MEPP) and 2-ethylhexanoic acid (2-EHA) were quantified in 62 workers and 29 controls from six factories. Analyses were performed by high-performance liquid chromatography with tandem mass spectrometry (HPLC-MS/MS) after on-line extraction.


RESULTS
Median concentrations of the pre- and post-shift urinary samples in the exposed workers were 12.6 and 28.7 μg/l for MEHP, 38.6 and 84.4 μg/l for 5cx-MEPP and 20.4 and 70.6 μg/l for 2-EHA, respectively. In the controls, the corresponding values were 4.8 and 4.7 μg/l for MEHP, 15.1 and 12.4 μg/l for 5cx-MEPP and 21.8 and 20.5 μg/l for 2-EHA, respectively. There was a significant increase (Mann-Whitney U-test, P < 0.05) of post-shift excretion in the exposed workers versus the unexposed controls and in the post-shift versus pre-shift concentrations only in the exposed workers. Values of 250 and 500 μg/l (100 and 280 μ/g creatinine) for MEHP and 5cx-MEPP, respectively, are proposed as guidance values.


CONCLUSIONS
There is clear evidence of occupational exposure of workers in these factories. The guideline values proposed should prevent high exposures in the soft PVC industry, particularly in factories where DEHP compounds or plastisols are employed. An epidemiological survey is needed to complete the DEHP risk assessment.
Gaudin
R
R
Département Polluants et Santé, Institut National de Recherche et de Sécurité, CS 60027, 54519 Vandoeuvre Cedex, France. rene.gaudin@inrs.fr
Marsan
P
P
Ndaw
S
S
Robert
A
A
Ducos
P
P
D002208:Caproates / Q000652:urine; D002617:Chemical Industry; D004051:Diethylhexyl Phthalate / Q000031:analogs & derivatives / Q000652:urine*; D004784:Environmental Monitoring / Q000379:methods*; D005260:Female; D005602:France; D006801:Humans; D008297:Male; D016273:Occupational Exposure / Q000032:analysis* / Q000517:prevention & control; D010795:Phthalic Acids / Q000652:urine; D010968:Plasticizers / Q000032:analysis*
D002208:Caproates; D010795:Phthalic Acids; D010968:Plasticizers; C479069:mono(2-ethyl-5-hydroxyhexyl) phthalate; C040668:2-ethylhexanoic acid; D004051:Diethylhexyl Phthalate; C016599:mono-(2-ethylhexyl)phthalate
Environ Health Perspect. 1997 Sep;105(9):972-8
9374082
Int J Hyg Environ Health. 2007 Oct;210(5):623-34
17889607
J Chromatogr B Biomed Sci Appl. 2001 Jul 15;758(2):213-9
11486831
Int J Androl. 2008 Apr;31(2):131-8
18070048
Xenobiotica. 1985 Mar;15(3):251-6
4024660
Environ Health Perspect. 2009 Apr;117(4):515-21
19440488
Arch Toxicol. 2005 Jul;79(7):367-76
15700144
Zentralbl Arbeitsmed Arbeitsschutz Prophyl. 1978 Dec;28(12):351-5
742241
Drug Metab Dispos. 1981 May-Jun;9(3):223-5
6113930
Food Addit Contam. 2001 Dec;18(12):1068-74
11761117
Environ Health Perspect. 2000 Oct;108(10):A440-2
11097556
Environ Health Perspect. 2007 Jul;115(7):1029-34
17637918
Bull Environ Contam Toxicol. 2004 Jun;72(6):1226-31
15362453
Environ Health Perspect. 1973 Jan;3:175-8
4704567
Environ Health Perspect. 2009 Jan;117(1):32-7
19165384
J Chromatogr B Analyt Technol Biomed Life Sci. 2005 Feb 25;816(1-2):269-80
15664359
IARC Monogr Eval Carcinog Risks Hum. 2000;77:41-148
11100399
J Chromatogr B Analyt Technol Biomed Life Sci. 2003 Jan 25;784(1):169-82
12504195
Environ Health Perspect. 2004 Mar;112(3):327-30
14998748
Int J Hyg Environ Health. 2007 May;210(3-4):319-33
17400024
Arch Toxicol. 2004 Mar;78(3):123-30
14576974
Environ Health Perspect. 2000 Oct;108(10):A440
11097555
Clin Chim Acta. 2005 Nov;361(1-2):20-9
16004980
J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Jun 5;805(1):49-56
15113539
Environ Health Perspect. 2004 Mar;112(3):331-8
14998749
Environ Health Perspect. 2008 Mar;116(3):334-9
18335100
Environ Health Perspect. 2006 Nov;114(11):1643-8
17107847
Ann Occup Hyg. 2009 Jan;53(1):1-17
18948546
Environ Health Perspect. 2001 May;109(5):437-42
11401753
Environ Health Perspect. 1982 Nov;45:11-7
7140682
Int Arch Occup Environ Health. 1993;64(8):555-60
8314613
Environ Health Perspect. 2000 Oct;108(10):979-82
11049818
Environ Health Perspect. 2004 Oct;112(14):1393-7
15471731
Biomarkers. 2006 Jan-Feb;11(1):1-13
16484133
Int J Hyg Environ Health. 2009 Sep;212(5):492-8
19423389
Environ Res. 2003 Oct;93(2):177-85
12963402
Environ Health Perspect. 2003 Jul;111(9):1148-51
12842765
Int Arch Occup Environ Health. 2005 Sep;78(8):663-9
16041606
J Chromatogr B Analyt Technol Biomed Life Sci. 2003 Jun 15;789(2):393-404
12742130
Anal Bioanal Chem. 2005 Jun;382(4):1084-92
15933851
Am Ind Hyg Assoc J. 1985 Nov;46(11):643-7
4072908
Occup Environ Med. 2005 Nov;62(11):806-18
16234408
Int Arch Occup Environ Health. 2008 Aug;81(8):959-66
18097680
Lancet. 1990 Mar 24;335(8691):725
1969080
Regul Toxicol Pharmacol. 2009 Dec;55(3):249-58
19751789
Environ Health Perspect. 2006 Feb;114(2):270-6
16451866