title: Interleukin-1β regulates the migratory potential of MDAMB231 breast cancer cells through the hypoxia-inducible factor-1α.
abstract: The casual relationship between inflammation and tumour progression has been widely accepted and the etiology of breast cancer has been associated with inflammatory processes. Interleukin (IL)-1β, besides its central role in inflammation, has also been recognised as a powerful player in tumour progression, angiogenesis and invasiveness. Recently, there has been considerable interest in understanding the non-hypoxic upregulation of the hypoxia-inducible factor (HIF)-1α by IL-1 in neoplastic cells since aberrant expression of HIF-1α correlates with tumour progression. Here, using the highly invasive human breast cancer cell line MDAMB231, we studied the effect of IL-1β on tumour cell migration along with HIF-1α accumulation. We observed that non-hypoxic induction of HIF-1α by IL-1β in MDAMB231 was associated with increased cell migration, paralleled by upregulation of p38 MAPK phosphorylation and CXCL8/CXCR1 expression. Inhibition of HIF-1α by siRNA resulted in a significant reduction of CXCR1 expression and IL-1β-induced cell migration in MDAMB231 cells, thus confirming a role of HIF-1α in the non-hypoxic-IL-1β-dependent induction of migratory potentials. Our observation that IL-1 induces HIF-1α accumulation in MDAMB231 cells was confirmed in tumour cells growing in vivo using an experimental approach, mimicking the endogenous release of IL-1 in mice bearing MDAMB231 xenografts. Our in vivo data, along with the fact that inhibition of HIF-1α resulted in the decrease of IL-1β-promoted cell migration, further support the link between inflammation and cancer. The overall results may have important implications in those therapeutic approaches aimed to inhibit IL-1-mediated activities in tumour cells, specifically in breast cancer.
authors.affiliation: Department of Physiology, University of Siena, Via Aldo Moro, 53100 Siena, Italy. Naldini@Unisi.it
authors.forename: Antonella
authors.initials: A
authors.lastname: Naldini
authors.forename: Irene
authors.initials: I
authors.lastname: Filippi
authors.forename: Diego
authors.initials: D
authors.lastname: Miglietta
authors.forename: Michele
authors.initials: M
authors.lastname: Moschetta
authors.forename: Raffaella
authors.initials: R
authors.lastname: Giavazzi
authors.forename: Fabio
authors.initials: F
authors.lastname: Carraro
mesh_terms: D000818:Animals; D001943:Breast Neoplasms / Q000378:metabolism*; D002450:Cell Communication / Q000502:physiology; D002465:Cell Movement / Q000502:physiology*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism*; D007093:Imidazoles / Q000494:pharmacology; D053583:Interleukin-1beta / Q000494:pharmacology / Q000502:physiology*; D016209:Interleukin-8 / Q000378:metabolism; D051379:Mice; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D009363:Neoplasm Proteins / Q000378:metabolism*; D009368:Neoplasm Transplantation; D011725:Pyridines / Q000494:pharmacology; D014183:Transplantation, Heterologous
chemical_list: D004791:Enzyme Inhibitors; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D007093:Imidazoles; D053583:Interleukin-1beta; D016209:Interleukin-8; D009363:Neoplasm Proteins; D011725:Pyridines; D020928:Mitogen-Activated Protein Kinases; C093642:SB 203580