title: Recording electric potentials from single adherent cells with 3D microelectrode arrays after local electroporation.
abstract: This short communication reports on the innovative method of the local micro-invasive needle electroporation (LOMINE) of single adherent cells. The investigation of cellular reactions in living cell cultures represents a fundamental method, e.g. for drug development and environmental monitoring. Existing classical methods for intracellular measurements using, e.g. patch clamp techniques are time-consuming and complex. Present patch-on-chip systems are limited to the investigation of single cells in suspension. Nevertheless, the most part of the cells of the human body is adherently growing. Therefore, we develop a new chip system for the growth of adherent cells with 64 micro-structured needle electrodes as well as 128 dielectrophoretic electrodes, located within a measuring area of 1 mm(2). With this analytical chip, the intracellular investigation of electro-chemical changes and processes in adherently growing cells will become possible in the near future. Here, we present first intracellular measurements with this chip system.
authors.affiliation: University of Rostock, Chair of Biophysics, Gertrudenstrasse 11a, 18057 Rostock, Germany. philipp.koester@uni-rostock.de
authors.forename: Philipp Julian
authors.initials: PJ
authors.lastname: Koester
authors.forename: Carsten
authors.initials: C
authors.lastname: Tautorat
authors.forename: Helmut
authors.initials: H
authors.lastname: Beikirch
authors.forename: Jan
authors.initials: J
authors.lastname: Gimsa
authors.forename: Werner
authors.initials: W
authors.lastname: Baumann
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D018274:Electroporation; D006801:Humans; D056656:Lab-On-A-Chip Devices; D008564:Membrane Potentials; D051379:Mice; D008839:Microelectrodes; D008855:Microscopy, Electron, Scanning; D018408:Patch-Clamp Techniques