Yeast extract, brewer's yeast and spirulina in diets for Labeo rohita fingerlings affect haemato-immunological responses and survival following Aeromonas hydrophila challenge.
A feeding trial was conducted for 60 days to study the immunomodulatory role of three different immunostimulants yeast extract (YE), brewer's yeast (BY) and spirulina (SP) in Labeo rohita fingerlings. Four hundred and fifty fingerlings (avg. wt 3.35±0.15 g) were randomly distributed in ten treatments and fed with either of ten iso-nitrogenous and iso-caloric semi-purified diets, prepared with three incremental levels (1%, 2% and 4%) of different immunostimulants except the control. Growth parameters did not vary significantly (p>0.05) among the experimental groups. Haematology and serum parameters was performed before Aeromonas hydrophila challenge whereas respiratory burst activity was analysed following challenge. The respiratory burst activity, total leucocyte count, serum total protein and globulin was significantly higher (p<0.05) in YE 1% supplemented group. The survival (%) after challenging with A. hydrophila was also highest in the YE fed groups. The results indicate that among the different sources and levels of immunostimulants, YE at lower inclusion level is more effective in promoting the immune status of L. rohita fingerlings.
Andrews
Simi Rose
SR
Department of Aquaculture, Central Institute of Fisheries Education, Versova, Mumbai 400061, India.
Sahu
N P
NP
Department of Fish Nutrition and Biochemistry, Central Institute of Fisheries Education, Versova, Mumbai 400061, India.
Pal
A K
AK
Department of Fish Nutrition and Biochemistry, Central Institute of Fisheries Education, Versova, Mumbai 400061, India.
Mukherjee
S C
SC
Department of Aquatic Animal Health Management, Central Institute of Fisheries Education, Versova, Mumbai 400061, India.
Kumar
Shivendra
S
Department of Fish Nutrition and Biochemistry, Central Institute of Fisheries Education, Versova, Mumbai 400061, India; Krishi Vigyan Kendra, Rajendra Agricultural University, Madhopur 845454, Bihar, India. Electronic address: shivdholi@rediffmail.com.
D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000494:pharmacology*; D016980:Aeromonas hydrophila; D000418:Albumins / Q000032:analysis; D000821:Animal Feed; D000818:Animals; D003530:Cyprinidae / Q000097:blood / Q000254:growth & development / Q000276:immunology*; D019587:Dietary Supplements; D004305:Dose-Response Relationship, Drug; D004906:Erythrocyte Count / Q000662:veterinary; D005916:Globulins / Q000032:analysis; D006454:Hemoglobins / Q000032:analysis; D055633:Immune System Phenomena / Q000187:drug effects*; D007958:Leukocyte Count / Q000662:veterinary; D011897:Random Allocation; D016897:Respiratory Burst / Q000187:drug effects; D053545:Spirulina; D016019:Survival Analysis; D015002:Yeast, Dried / Q000008:administration & dosage / Q000494:pharmacology*
D000276:Adjuvants, Immunologic; D000418:Albumins; D005916:Globulins; D006454:Hemoglobins