title: Randomized clinical trial of the influence of mechanical bowel preparation on faecal microflora in patients undergoing colonic cancer resection.
abstract: BACKGROUND
  This study investigated the influence of mechanical bowel preparation (MBP) on faecal microflora, using rRNA-targeted reverse transcription-quantitative polymerase chain reaction in patients undergoing colonic cancer resection.
  
  
  METHODS
  Forty-two patients undergoing elective colonic surgery were randomized into MBP or no-MBP groups (21 in each group). The main outcome was the bacterial microflora and faecal organic acid content of faecal material obtained at operation.
  
  
  RESULTS
  Clinical characteristics were similar in the two groups. Bowel content in the resected specimens did not differ significantly. The count of bacterial microflora, such as Bifidobacterium and total Lactobacillus, in both intraoperative faecal material and first material after surgery was significantly lower in the MBP group than the no-MBP group (P < 0·050). Levels of faecal organic acids, such as acetic acid, propionic acid and butyric acid, in intraoperative faecal material were significantly lower, and levels of lactic acid were significantly higher, in the MBP group than in the no-MBP group (P < 0·050). The succinic acid level was significantly higher after surgery than before operation in the MBP group (P = 0·008).
  
  
  CONCLUSIONS
  Preoperative MBP caused an imbalance in the bowel microflora, suggesting that it offers no advantages in terms of enterobacterial microflora for patients undergoing colonic cancer resection.
  
  
  BACKGROUND
  UMIN000003153 (http://www.umin.ac.jp/ctr/index.htm).
authors.affiliation: Department of Gastroenterological and General Surgery, Showa University School of Medicine, Tokyo, Japan. FZA05334@nifty.com
authors.forename: M
authors.initials: M
authors.lastname: Watanabe
authors.forename: M
authors.initials: M
authors.lastname: Murakami
authors.forename: K
authors.initials: K
authors.lastname: Nakao
authors.forename: T
authors.initials: T
authors.lastname: Asahara
authors.forename: K
authors.initials: K
authors.lastname: Nomoto
authors.forename: A
authors.initials: A
authors.lastname: Tsunoda
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D019072:Antibiotic Prophylaxis; D002400:Cathartics / Q000627:therapeutic use*; D019343:Citric Acid / Q000627:therapeutic use*; D003110:Colonic Neoplasms / Q000601:surgery*; D004733:Enema; D005243:Feces / Q000382:microbiology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009942:Organometallic Compounds / Q000627:therapeutic use*; D011300:Preoperative Care / Q000379:methods*; D020127:Recovery of Function; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016896:Treatment Outcome
chemical_list: D002400:Cathartics; D009942:Organometallic Compounds; D019343:Citric Acid; C110422:magnesium citrate