title: Cellular microparticles as a marker for cerebral vasospasm in spontaneous subarachnoid hemorrhage.
abstract: OBJECTIVE
  Spontaneous subarachnoid hemorrhage (SAH) still has a high risk for poor outcome that is frequently attributable to symptomatic cerebral vasospasm (CVS). We hypothesize that cellular microparticles (MP) play a role in the pathogenesis of CVS and may serve as biomarkers for CVS.
  
  
  METHODS
  In 20 consecutive SAH patients, endothelial, leukocyte, platelet, and erythrocyte MP were measured during 15 days after ictus. CVS was detected by transcranial Doppler sonography. Twenty matched volunteers served as healthy controls.
  
  
  RESULTS
  Endothelial, leukocyte, and erythrocyte MP were elevated in SAH patients compared to healthy controls. CD105(+) and CD62e(+) endothelial MP were significantly higher in SAH patients with Doppler sonographic CVS. CD105(+) endothelial MP were especially increased on the days of Doppler sonographic CVS onset. In patients experiencing cerebral infarction attributable to vasospasm, CD41(+) platelet MP were elevated in addition to endothelial MP. CD41(+) platelet MP were significantly higher in patients with any level of disability (modified Rankin Scale score ≥1) compared to those who made a full recovery (modified Rankin Scale score=0) on discharge from hospital.
  
  
  CONCLUSIONS
  Endothelial MP were elevated in patients with SAH. This elevation coincided with the occurrence of Doppler sonographic CVS and therefore could be a novel biomarker for CVS. Platelet MP might be involved in the pathogenesis of cerebral infarction attributable to vasospasm, resulting in neurological morbidity.
authors.affiliation: Department of Neurology, Innsbruck Medical University, Anichstrasse 35, 6020 Innsbruck, Austria. peter.lackner@i-med.ac.at
authors.forename: Peter
authors.initials: P
authors.lastname: Lackner
authors.forename: Anelia
authors.initials: A
authors.lastname: Dietmann
authors.forename: Ronny
authors.initials: R
authors.lastname: Beer
authors.forename: Marlene
authors.initials: M
authors.lastname: Fischer
authors.forename: Gregor
authors.initials: G
authors.lastname: Broessner
authors.forename: Raimund
authors.initials: R
authors.lastname: Helbok
authors.forename: Johannes
authors.initials: J
authors.lastname: Marxgut
authors.forename: Bettina
authors.initials: B
authors.lastname: Pfausler
authors.forename: Erich
authors.initials: E
authors.lastname: Schmutzhard
mesh_terms: D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000378:metabolism; D055252:Cell-Derived Microparticles / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011237:Predictive Value of Tests; D011446:Prospective Studies; D013345:Subarachnoid Hemorrhage / Q000097:blood* / Q000150:complications; D017585:Ultrasonography, Doppler, Transcranial; D020301:Vasospasm, Intracranial / Q000097:blood* / Q000000981:diagnostic imaging / Q000209:etiology
chemical_list: D015415:Biomarkers