title: Gene expression following induction of regeneration in Drosophila wing imaginal discs. Expression profile of regenerating wing discs.
abstract: BACKGROUND
  Regeneration is the ability of an organism to rebuild a body part that has been damaged or amputated, and can be studied at the molecular level using model organisms. Drosophila imaginal discs, which are the larval primordia of adult cuticular structures, are capable of undergoing regenerative growth after transplantation and in vivo culture into the adult abdomen.
  
  
  RESULTS
  Using expression profile analyses, we studied the regenerative behaviour of wing discs at 0, 24 and 72 hours after fragmentation and implantation into adult females. Based on expression level, we generated a catalogue of genes with putative role in wing disc regeneration, identifying four classes: 1) genes with differential expression within the first 24 hours; 2) genes with differential expression between 24 and 72 hours; 3) genes that changed significantly in expression levels between the two time periods; 4) genes with a sustained increase or decrease in their expression levels throughout regeneration. Among these genes, we identified members of the JNK and Notch signalling pathways and chromatin regulators. Through computational analysis, we recognized putative binding sites for transcription factors downstream of these pathways that are conserved in multiple Drosophilids, indicating a potential relationship between members of the different gene classes. Experimental data from genetic mutants provide evidence of a requirement of selected genes in wing disc regeneration.
  
  
  CONCLUSIONS
  We have been able to distinguish various classes of genes involved in early and late steps of the regeneration process. Our data suggests the integration of signalling pathways in the promoters of regulated genes.
authors.affiliation: Departament de Genètica, and Institut de Biomedicina de la Universitat de Barcelona, Diagonal 645, 08028 Barcelona, Catalonia, Spain.
authors.forename: Enrique
authors.initials: E
authors.lastname: Blanco
authors.forename: Marina
authors.initials: M
authors.lastname: Ruiz-Romero
authors.forename: Sergi
authors.initials: S
authors.lastname: Beltran
authors.forename: Manel
authors.initials: M
authors.lastname: Bosch
authors.forename: Adrià
authors.initials: A
authors.lastname: Punset
authors.forename: Florenci
authors.initials: F
authors.lastname: Serras
authors.forename: Montserrat
authors.initials: M
authors.lastname: Corominas
mesh_terms: D000818:Animals; D042002:Chromatin Assembly and Disassembly; D004330:Drosophila / Q000235:genetics / Q000502:physiology*; D029721:Drosophila Proteins / Q000378:metabolism; D020869:Gene Expression Profiling; D012038:Regeneration; D015398:Signal Transduction; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic; D014921:Wings, Animal / Q000502:physiology
chemical_list: D029721:Drosophila Proteins; D014157:Transcription Factors
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