title: Tissue-engineered conduit using urine-derived stem cells seeded bacterial cellulose polymer in urinary reconstruction and diversion.
abstract: The objective of this study was to generate bacterial cellulose (BC) scaffolds seeded with human urine-derived stem cells (USC) to form a tissue-engineered conduit for use in urinary diversion. Microporous BC scaffolds were synthesized and USC were induced to differentiate into urothelial and smooth muscle cells (SMC). Induced USC (10(6) cells/cm(2)) were seeded onto BC under static and 3D dynamic (10 or 40 RPM) conditions and cultured for 2 weeks. The urothelial cells and SMC derived from USC formed multilayers on the BC scaffold surface, and some cells infiltrated into the scaffold. The urothelium derived from USC differentiation expressed urothelial markers (uroplakin Ia and AE1/AE3) and the SMC expressed SMC markers (α-smooth muscle actin and desmin). In addition, USC/BC scaffold constructs were implanted into athymic mice, and the cells were tracked using immunohistochemical staining for human nuclear antigen. In vivo, the cells appeared to differentiate and express urothelial and SMC markers. In conclusion, porous BC scaffolds allow 3 dimensional growth of USC, leading to formation of a multilayered urothelium and cell-matrix infiltration. Thus, cell-seeded BC scaffolds hold promise for use in tissue-engineered urinary conduits for urinary reconstruction.
authors.affiliation: Chalmers University of Technology, BBV laboratory, Sweden.
authors.forename: Aase
authors.initials: A
authors.lastname: Bodin
authors.forename: Shantaram
authors.initials: S
authors.lastname: Bharadwaj
authors.forename: Shaofeng
authors.initials: S
authors.lastname: Wu
authors.forename: Paul
authors.initials: P
authors.lastname: Gatenholm
authors.forename: Anthony
authors.initials: A
authors.lastname: Atala
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000091:Acetobacter / Q000737:chemistry; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002478:Cells, Cultured; D002482:Cellulose / Q000494:pharmacology* / Q000648:ultrastructure; D018920:Coculture Techniques; D055119:Elastic Modulus / Q000187:drug effects; D004731:Endotoxins / Q000378:metabolism; D005109:Extracellular Matrix / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects; D051379:Mice; D020286:Pericytes / Q000166:cytology / Q000187:drug effects; D016062:Porosity / Q000187:drug effects; D019919:Prosthesis Implantation; D019651:Plastic Surgery Procedures / Q000379:methods*; D013718:Tensile Strength / Q000187:drug effects; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*; D014547:Urinary Diversion / Q000379:methods*; D014556:Urine / Q000166:cytology*
chemical_list: D015415:Biomarkers; D004731:Endotoxins; D002482:Cellulose