The impact of acute strenuous exercise on TLR2, TLR4 and HLA.DR expression on human blood monocytes induced by autologous serum.
Acute exercise alters the surface expression of toll-like receptors (TLRs) and HLA.DR on blood monocytes, which could transiently compromise immunity. As serum factors might be responsible, we examined the effects of autologous post-exercise serum exposure on TLR2, TLR4 and HLA.DR expression on resting blood monocytes and their subtypes. Eight trained cyclists completed an ergometer 60 km time trial. PBMCs and serum were obtained before, immediately after and 1 h after exercise. TLR2, TLR4 or HLA.DR expression (gMFI) was determined on blood monocyte subtypes expressing combinations of CD14 and CD16 by flow cytometry, and on resting monocytes exposed to 50% autologous serum (pre, immediately after or 1 h after exercise) for 18 h in culture. Immediately after exercise, total monocyte expression of TLR2 and TLR4 increased by 41 and 27%, respectively, while HLA.DR expression was 39% lower than baseline. TLR2 and TLR4 was 53 and 84% greater 1 h after exercise, respectively, while HLA.DR was 48% lower. Changes in TLR2 and TLR4 expression occurred on the CD14(++bright)/CD16(+dim) monocyte subtype only, while HLA.DR expression changed on the CD14(+dim)/CD16(++bright) subtype. Serum did not affect monocyte TLR2 or TLR4 expression but 1 h post serum increased expression of HLA.DR on total monocytes and the CD14(+dim)/CD16(++bright) subtype, which was in contrast to the change observed at this time after exercise. We conclude that a bout of strenuous aerobic exercise alters the surface expression of TLR2, TLR4 and HLA.DR on blood monocytes and some of their subtypes, but these changes appear to be unrelated to blood serum factors.
Booth
Stephen
S
Biomedicine and Sports Science Research Group, School of Life Sciences, Edinburgh Napier University, 10 Colinton Road, Edinburgh, EH10 5DT, UK.
Florida-James
Geraint D
GD
McFarlin
Brian K
BK
Spielmann
Guillaume
G
O'Connor
Daniel P
DP
Simpson
Richard J
RJ
D000328:Adult; D000332:Aerobiosis / Q000502:physiology; D002478:Cells, Cultured; D015444:Exercise / Q000502:physiology*; D005260:Female; D005434:Flow Cytometry; D006684:HLA-DR Antigens / Q000378:metabolism*; D006801:Humans; D008297:Male; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism*; D005082:Physical Exertion / Q000502:physiology*; D044967:Serum / Q000502:physiology*; D051195:Toll-Like Receptor 2 / Q000378:metabolism*; D051197:Toll-Like Receptor 4 / Q000378:metabolism*
D006684:HLA-DR Antigens; C495342:TLR2 protein, human; C495345:TLR4 protein, human; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4
Am J Physiol Regul Integr Comp Physiol. 2009 Mar;296(3):R575-86
19158413
Clin Exp Immunol. 2007 Jan;147(1):184-8
17177978
Intensive Care Med. 2006 Aug;32(8):1175-83
16741700
Brain Behav Immun. 2008 May;22(4):590-9
18242048
Cytokine. 2007 Nov;40(2):75-81
17919919
Ann Acad Med Singap. 2008 Apr;37(4):307-14
18461215
Scand J Immunol. 2008 Feb;67(2):152-9
18201370
Clin Immunol. 2006 May;119(2):180-7
16517212
Diagn Pathol. 2009 Apr 16;4:12
19371402
J Physiol. 2005 Mar 15;563(Pt 3):945-55
15661814
Am J Physiol Cell Physiol. 2001 Apr;280(4):C769-74
11245592
Brain Behav Immun. 2009 Feb;23(2):232-9
18930806
Crit Care Med. 2008 Apr;36(4):1267-76
18379254
Nature. 2000 Dec 7;408(6813):740-5
11130078
Clin Hemorheol Microcirc. 2004;31(3):217-26
15322327
Free Radic Biol Med. 2003 Aug 1;35(3):284-91
12885590
Appl Physiol Nutr Metab. 2009 Jun;34(3):454-8
19448714
Eur J Appl Physiol. 2010 May;109(2):251-7
20063104
Clin Exp Immunol. 2004 May;136(2):312-9
15086396
Clin Exp Immunol. 2005 Aug;141(2):270-8
15996191
Brain Behav Immun. 2008 Oct;22(7):1066-1071
18477503
Microbes Infect. 2006 Aug;8(9-10):2492-500
16872859
Blood. 1989 Nov 15;74(7):2527-34
2478233
J Leukoc Biol. 2007 Mar;81(3):584-92
17135573
Exerc Immunol Rev. 2006;12:34-53
17201071
Am J Physiol Cell Physiol. 2000 Sep;279(3):C578-86
10942707
Brain Behav Immun. 2006 Nov;20(6):578-84
16554145