title: The Friedreich's ataxia protein frataxin modulates DNA base excision repair in prokaryotes and mammals.
abstract: DNA-repair mechanisms enable cells to maintain their genetic information by protecting it from mutations that may cause malignant growth. Recent evidence suggests that specific DNA-repair enzymes contain ISCs (iron-sulfur clusters). The nuclearencoded protein frataxin is essential for the mitochondrial biosynthesis of ISCs. Frataxin deficiency causes a neurodegenerative disorder named Friedreich's ataxia in humans. Various types of cancer occurring at young age are associated with this disease, and hence with frataxin deficiency. Mice carrying a hepatocyte-specific disruption of the frataxin gene develop multiple liver tumours for unresolved reasons. In the present study, we show that frataxin deficiency in murine liver is associated with increased basal levels of oxidative DNA base damage. Accordingly, eukaryotic V79 fibroblasts overexpressing human frataxin show decreased basal levels of these modifications, while prokaryotic Salmonella enterica serotype Typhimurium TA104 strains transformed with human frataxin show decreased mutation rates. The repair rates of oxidative DNA base modifications in V79 cells overexpressing frataxin were significantly higher than in control cells. Lastly, cleavage activity related to the ISC-independent repair enzyme 8-oxoguanine glycosylase was found to be unaltered by frataxin overexpression. These findings indicate that frataxin modulates DNA-repair mechanisms probably due to its impact on ISC-dependent repair proteins, linking mitochondrial dysfunction to DNA repair and tumour initiation.
authors.affiliation: Department of Food Toxicology and Replacement/Complementary Methods to Animal Testing, University of Veterinary Medicine Hannover, Germany. rene.thierbach@uni-jena.de
authors.forename: René
authors.initials: R
authors.lastname: Thierbach
authors.forename: Gunnar
authors.initials: G
authors.lastname: Drewes
authors.forename: Markus
authors.initials: M
authors.lastname: Fusser
authors.forename: Anja
authors.initials: A
authors.lastname: Voigt
authors.forename: Doreen
authors.initials: D
authors.lastname: Kuhlow
authors.forename: Urte
authors.initials: U
authors.lastname: Blume
authors.forename: Tim J
authors.initials: TJ
authors.lastname: Schulz
authors.forename: Carina
authors.initials: C
authors.lastname: Reiche
authors.forename: Hansruedi
authors.initials: H
authors.lastname: Glatt
authors.forename: Bernd
authors.initials: B
authors.lastname: Epe
authors.forename: Pablo
authors.initials: P
authors.lastname: Steinberg
authors.forename: Michael
authors.initials: M
authors.lastname: Ristow
mesh_terms: D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D004249:DNA Damage; D045647:DNA Glycosylases / Q000378:metabolism; D004260:DNA Repair / Q000235:genetics*; D005347:Fibroblasts / Q000378:metabolism / Q000473:pathology; D005621:Friedreich Ataxia / Q000235:genetics* / Q000378:metabolism; D022781:Hepatocytes / Q000378:metabolism / Q000473:pathology; D006801:Humans; D033862:Iron-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D007506:Iron-Sulfur Proteins / Q000378:metabolism; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D008322:Mammals / Q000235:genetics / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D009154:Mutation; D018384:Oxidative Stress; D011387:Prokaryotic Cells / Q000378:metabolism; D019779:Salmonella enterica / Q000235:genetics; D014162:Transfection; D000096923:Frataxin
chemical_list: D045647:DNA Glycosylases; D033862:Iron-Binding Proteins; D007506:Iron-Sulfur Proteins; D000096923:Frataxin; C479058:oxoguanine glycosylase 1, human
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