Temperature-dependent requirement for catalase in aerobic growth of Listeria monocytogenes F2365.
Listeria monocytogenes is a Gram-positive, psychrotrophic, facultative intracellular food-borne pathogen responsible for severe illness (listeriosis). The bacteria can grow in a wide range of temperatures (1 to 45°C), and low-temperature growth contributes to the food safety hazards associated with contamination of ready-to-eat foods with this pathogen. To assess the impact of oxidative stress responses on the ability of L. monocytogenes to grow at low temperatures and to tolerate repeated freeze-thaw stress (cryotolerance), we generated and characterized a catalase-deficient mutant of L. monocytogenes F2365 harboring a mariner-based transposon insertion in the catalase gene (kat). When grown aerobically on blood-free solid medium, the kat mutant exhibited impaired growth, with the extent of impairment increasing with decreasing temperature, and no growth was detected at 4°C. Aerobic growth in liquid was impaired at 4°C, especially under aeration, but not at higher temperatures (10, 25, or 37°C). Genetic complementation of the mutant with the intact kat restored normal growth, confirming that inactivation of this gene was responsible for the growth impairment. In spite of the expected impact of oxidative stress responses on cryotolerance, cryotolerance of the kat mutant was not affected.
Azizoglu
Reha Onur
RO
Department of Food, Bioprocessing and Nutrition Sciences, North Carolina State University, Schaub Hall, Raleigh, NC 27695-7624, USA.
Kathariou
Sophia
S
D000332:Aerobiosis / Q000502:physiology; D000818:Animals; D002374:Catalase / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D002642:Chick Embryo / Q000382:microbiology; D003470:Culture Media; D005516:Food Microbiology; D005615:Freezing; D008089:Listeria monocytogenes / Q000201:enzymology / Q000235:genetics / Q000254:growth & development* / Q000502:physiology; D009154:Mutation; D013696:Temperature
D003470:Culture Media; D002374:Catalase
PLoS Pathog. 2009 May;5(5):e1000449
19478867
J Dairy Sci. 1980 Jul;63(7):1199-204
6999043
Infect Immun. 2006 Feb;74(2):1323-38
16428782
Appl Environ Microbiol. 2005 Dec;71(12):8314-22
16332818
Appl Environ Microbiol. 2009 Aug;75(16):5315-20
19542335
Microbiology (Reading). 2004 Feb;150(Pt 2):497-512
14766928
J Clin Microbiol. 1998 Sep;36(9):2530-4
9705387
J Bacteriol. 1999 Aug;181(16):5024-32
10438775
Bioinformatics. 2005 Aug 15;21(16):3422-3
15976072
J Food Prot. 2002 Nov;65(11):1811-29
12430709
J Bacteriol. 2002 Aug;184(15):4177-86
12107135
Clin Infect Dis. 2000 Sep;31(3):770-5
11017828
Scand J Immunol. 1993 Apr;37(4):443-6
8469926
Nucleic Acids Res. 2004 Apr 28;32(8):2386-95
15115801
Curr Microbiol. 2009 Feb;58(2):134-8
18956226
Microbiology (Reading). 2002 Jun;148(Pt 6):1855-1862
12055305
J Clin Microbiol. 2006 May;44(5):1917-8
16672441
ILAR J. 2000;41(4):187-96
11123179
Appl Environ Microbiol. 2000 Jul;66(7):3110-2
10877815
Mol Microbiol. 2002 Jul;45(1):9-15
12100544
Eur J Clin Microbiol Infect Dis. 1997 Nov;16(11):839-42
9447907
Infect Immun. 2004 Feb;72(2):1072-83
14742555
Am J Clin Pathol. 1991 Jul;96(1):130-3
1906240
Microbiol Rev. 1991 Sep;55(3):476-511
1943998
Clin Microbiol Rev. 2001 Jul;14(3):584-640
11432815
J Clin Microbiol. 1997 Jan;35(1):179-83
8968903
Infect Immun. 1989 Aug;57(8):2569-73
2545629
Microbes Infect. 2007 Aug;9(10):1236-43
17720602
J Food Prot. 2010 Aug;73(8):1474-9
20819357
Eur J Clin Microbiol Infect Dis. 1996 Dec;15(12):965-7
9031886
J Clin Microbiol. 1978 May;7(5):474-8
350896
Appl Environ Microbiol. 2007 Apr;73(8):2758-61
17308180
Infect Immun. 1979 Mar;23(3):863-72
110685
J Bacteriol. 1991 Aug;173(16):5159-67
1860824
Appl Environ Microbiol. 2008 Mar;74(5):1653-5
18192405