[Scl1 of M41-type group A streptococcus binds low-density lipoprotein].
OBJECTIVE
To study the interaction of streptococcal collagen-like protein 1 (Scl1) of M41-type group A streptococcus (GAS) ATCC12373 with low-density lipoprotein (LDL).


METHODS
We cloned, expressed and purified the recombinant proteins rScl1 and its V region rScl1-V, designated as C176 and C176V, derived from Scl1. 41 of M41-type GAS. The binding of rScl1 to LDL was detected with affinity chromatography-binding assay, Western blot analysis and enzyme-linked immunosorbent assay (ELISA), and whole cell binding assays were used to detect the interactions of whole GAS cells with LDL.


RESULTS
The results demonstrated that C176 and C176V could specifically bind purified LDL, and M41-type GAS cells expressing native Sell could bind LDL whereas M6-type GAS could not.


CONCLUSIONS
The Sell of M41-type GAS specifically binds LDL.
Liang
Chunwei
C
College of Life Sciences, Inner Mongolia Agricultural University, Hohhot 010018, China. chunweiliang@yahoo.cn
Zhao
Ruidong
R
Gao
Yuming
Y
Han
Runlin
R
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D003094:Collagen / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008969:Molecular Sequence Data; D011485:Protein Binding; D016415:Sequence Alignment; D013290:Streptococcal Infections / Q000378:metabolism*; D013297:Streptococcus pyogenes / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
D001426:Bacterial Proteins; D008077:Lipoproteins, LDL; C423454:Scl1 protein, Streptococcus; D003094:Collagen