Analysis of the effects of androgens and training on myostatin propeptide and follistatin concentrations in blood and skeletal muscle using highly sensitive immuno PCR.
Myostatin propeptide (MYOPRO) and follistatin (FOLLI) are potent myostatin inhibitors. In this study we analysed effects of training and androgens on MYOPRO and FOLLI concentrations in blood and skeletal muscle using Immuno PCR. Young healthy males performed either a 3-month endurance training or a strength training. Blood and biopsy samples were analysed. Training did not significantly affect MYOPRO and FOLLI concentrations in serum and muscle. To investigate whether total skeletal muscle mass may affect circulating MYOPRO and FOLLI levels, blood samples of tetraplegic patients, untrained volunteers and bodybuilders were analysed. MYOPRO was significantly increased exclusively in the bodybuilder group. In orchiectomised rats MYOPRO increased in blood and muscle after treatment with testosterone. In summary our data demonstrate that moderate training does not affect the concentrations of MYOPRO to FOLLI. In contrast androgen treatment results in a significant increase of MYOPRO in skeletal muscle and serum.
Diel
Patrick
P
Centre of Preventive Doping Research, Dept. of Molecular and Cellular Sports Medicine, German Sport University Cologne, Cologne, Germany. diel@dshs-koeln.de
Schiffer
Thorsten
T
Geisler
Stephan
S
Hertrampf
Torsten
T
Mosler
Stephanie
S
Schulz
Sven
S
Wintgens
Karl Florian
KF
Adler
Michael
M
D000728:Androgens / Q000494:pharmacology*; D000818:Animals; D001706:Biopsy; D015153:Blotting, Western; D038681:Follistatin / Q000097:blood* / Q000276:immunology; D006801:Humans; D008297:Male; D018482:Muscle, Skeletal / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D055435:Myostatin / Q000097:blood* / Q000276:immunology; D009919:Orchiectomy; D009929:Organ Size / Q000187:drug effects; D010805:Physical Conditioning, Animal; D010806:Physical Education and Training; D016133:Polymerase Chain Reaction / Q000379:methods*; D011498:Protein Precursors / Q000097:blood* / Q000276:immunology; D051381:Rats; D017208:Rats, Wistar; D013739:Testosterone / Q000494:pharmacology; D055815:Young Adult
D000728:Androgens; D038681:Follistatin; D055435:Myostatin; D011498:Protein Precursors; D013739:Testosterone