Polymeric nanopillar arrays for cell traction force measurements.
This paper reports the development of a novel force measurement device based on polymeric nanopillar arrays. The device was fabricated by a process combining nanosphere lithography, oxygen plasma treatment, deep etching and nano-molding. Well-ordered polymeric nanopillar arrays with various diameters and aspect ratios were fabricated and used as cell culture substrates. Cell traction forces were measured by the deflection of the nanopillars. Since the location of the nanopillars can be monitored at all times, this device allows for the measurement of the evolution of adhesion forces over time.
Kuo
Chiung-Wen
CW
Research Center for Applied Sciences, Academia Sinica, Taipei, Taiwan.
Shiu
Jau-Ye
JY
Chien
Fan-Ching
FC
Tsai
Shih-Min
SM
Chueh
Di-Yan
DY
Chen
Peilin
P
D000818:Animals; D001696:Biomechanical Phenomena / Q000502:physiology; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D004852:Epoxy Compounds; D022001:Focal Adhesions / Q000502:physiology*; D017276:Friction; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D011108:Polymers; D011137:Polystyrenes / Q000737:chemistry*; D045663:Quantum Dots; D012825:Silicon; D013314:Stress, Mechanical
D004852:Epoxy Compounds; D011108:Polymers; D011137:Polystyrenes; C520895:SU-8 compound; D012825:Silicon