title: A survey of porcine picornaviruses and adenoviruses in fecal samples in Spain.
abstract: In the course of an epidemiologic surveillance program for swine diseases carried out in Spain, 206 cytopathic viruses were isolated from 600 porcine fecal samples between 2004 and 2005. The virus isolates were examined using reverse transcription polymerase chain reaction (RT-PCR) methods specific for different types of porcine picornaviruses, including members of the Teschovirus, Enterovirus, and Sapelovirus genera, and PCR for porcine adenoviruses. Of the 206 isolates, 97 (47%) were identified as teschoviruses, 18 (9%) as sapeloviruses, and 7 (3%) as porcine adenoviruses. Neither Porcine enterovirus B nor Swine vesicular disease virus was found among the isolates. The present study confirms that teschoviruses are highly prevalent in porcine fecal samples, at least in Spain. It also reveals that these viruses commonly circulate among apparently healthy pigs.
authors.affiliation: Laboratorio Central de Veterinaria, Algete, Spain.
authors.forename: Dolores
authors.initials: D
authors.lastname: Buitrago
authors.forename: Cristina
authors.initials: C
authors.lastname: Cano-Gómez
authors.forename: Montserrat
authors.initials: M
authors.lastname: Agüero
authors.forename: Paloma
authors.initials: P
authors.lastname: Fernandez-Pacheco
authors.forename: Concepción
authors.initials: C
authors.lastname: Gómez-Tejedor
authors.forename: Miguel Angel
authors.initials: MA
authors.lastname: Jiménez-Clavero
mesh_terms: D000256:Adenoviridae / Q000145:classification / Q000302:isolation & purification*; D000818:Animals; D002460:Cell Line; D004770:Enterovirus / Q000235:genetics / Q000302:isolation & purification; D005243:Feces / Q000821:virology*; D006801:Humans; D007668:Kidney / Q000821:virology; D010849:Picornaviridae / Q000145:classification / Q000302:isolation & purification*; D012016:Reference Values; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012703:Serotyping; D013030:Spain; D013552:Swine / Q000821:virology*; D013553:Swine Diseases / Q000145:classification / Q000821:virology*; D052700:Teschovirus / Q000145:classification / Q000302:isolation & purification