Alpha-lipoic acid modulates GFAP, vimentin, nestin, cyclin D1 and MAP-kinase expression in astroglial cell cultures.
In the present study, we evaluated the expression of some proliferation and differentiation markers in 15 DIV astrocyte cultures pretreated or not with 0.5 mM glutamate for 24 h and than maintained under chronic or acute treatment with 50 μM R(+)enantiomer or raceme alpha-lipoic acid (ALA). GFAP expression significantly increased after (R+)enantiomer acute-treatment and also in glutamate-pretreated ones. Vimentin expression increased after R(+)enantiomer acute-treatment, but it decreased after raceme acute-treatment. Nestin expression drastically increased after acute raceme-treatment in glutamate-pretreated or not cultures, but significantly decreased after (R+)enantiomer acute and chronic-treatments. Cyclin D1 expression increased in raceme acute-treated cultures pretreated with glutamate. MAP-kinase expression slightly increased after (R+)enantiomer acute treatment in glutamate-pretreated or unpretreated ones. These preliminary findings may better clarify antioxidant and metabolic role played by ALA in proliferating and differentiating astrocyte cultures suggesting an interactive cross-talk between glial and neuronal cells, after brain lesions or damages.
Bramanti
V
V
Dept of Chemical Sciences, Section of Biochemistry and Molecular biology, University of Catania, Viale A Doria 6, 95125 Catania, Italy.
Tomassoni
D
D
Bronzi
D
D
Grasso
S
S
Currò
M
M
Avitabile
M
M
Li Volsi
G
G
Renis
M
M
Ientile
R
R
Amenta
F
F
Avola
R
R
D000818:Animals; D001253:Astrocytes / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D015153:Blotting, Western; D002478:Cells, Cultured; D019938:Cyclin D1 / Q000378:metabolism*; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism*; D007381:Intermediate Filament Proteins / Q000378:metabolism*; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D064231:Nestin; D051381:Rats; D017208:Rats, Wistar; D008063:Thioctic Acid / Q000494:pharmacology*; D014746:Vimentin / Q000378:metabolism*
D005904:Glial Fibrillary Acidic Protein; D007381:Intermediate Filament Proteins; D009419:Nerve Tissue Proteins; C577486:Nes protein, rat; D064231:Nestin; D014746:Vimentin; D019938:Cyclin D1; D008063:Thioctic Acid; D020928:Mitogen-Activated Protein Kinases
Neurochem Res. 2008 Dec;33(12):2593-600
18612815
Am J Physiol. 1999 May;276(5):R1327-33
10233023
J Med Chem. 2007 Mar 8;50(5):951-61
17328523
Free Radic Biol Med. 2002 Jul 1;33(1):83-93
12086686
Biochem Pharmacol. 1995 Jun 29;50(1):123-6
7605337
Proc Natl Acad Sci U S A. 2004 Mar 9;101(10):3381-6
14985508
Diabetes. 1996 Dec;45(12):1798-804
8922368
Biochem Biophys Res Commun. 1995 Jan 17;206(2):724-30
7826393
Glia. 1999 Apr;26(2):97-108
10384875
Neurochem Res. 2007 Jan;32(1):49-56
17151914
J Cereb Blood Flow Metab. 1995 Jul;15(4):624-30
7790411
Diabetes Technol Ther. 2000 Autumn;2(3):401-13
11467343
Front Biosci (Schol Ed). 2010 Jan 01;2:558-70
20036968
J Clin Pharmacol. 2003 Nov;43(11):1257-67
14551180
Curr Med Chem. 2004 May;11(9):1135-46
15134511
Free Radic Biol Med. 1997;22(1-2):359-78
8958163
Endocr Metab Immune Disord Drug Targets. 2008 Jun;8(2):132-42
18537699
J Biol Chem. 2001 Oct 19;276(42):38329-36
11477096