title: The recruitment of acetylated and unacetylated tropomyosin to distinct actin polymers permits the discrete regulation of specific myosins in fission yeast.
abstract: Tropomyosin (Tm) is a conserved dimeric coiled-coil protein, which forms polymers that curl around actin filaments in order to regulate actomyosin function. Acetylation of the Tm N-terminal methionine strengthens end-to-end bonds, which enhances actin binding as well as the ability of Tm to regulate myosin motor activity in both muscle and non-muscle cells. In this study we explore the function of each Tm form within fission yeast cells. Electron microscopy and live cell imaging revealed that acetylated and unacetylated Tm associate with distinct actin structures within the cell, and that each form has a profound effect upon the shape and integrity of the polymeric actin filament. We show that, whereas Tm acetylation is required to regulate the in vivo motility of class II myosins, acetylated Tm had no effect on the motility of class I and V myosins. These findings illustrate a novel Tm-acetylation-state-dependent mechanism for regulating specific actomyosin cytoskeletal interactions.
authors.affiliation: School of Biosciences, University of Kent, Canterbury, Kent, CT2 7NJ, UK.
authors.forename: Arthur T
authors.initials: AT
authors.lastname: Coulton
authors.forename: Daniel A
authors.initials: DA
authors.lastname: East
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Galinska-Rakoczy
authors.forename: William
authors.initials: W
authors.lastname: Lehman
authors.forename: Daniel P
authors.initials: DP
authors.lastname: Mulvihill
mesh_terms: D000107:Acetylation; D000123:Acetyltransferases / Q000235:genetics; D000199:Actins / Q000378:metabolism*; D002453:Cell Cycle / Q000235:genetics; D018797:Cell Cycle Proteins / Q000737:chemistry / Q000378:metabolism*; D003599:Cytoskeleton / Q000378:metabolism; D009218:Myosins / Q000378:metabolism; D011485:Protein Binding / Q000235:genetics; D012568:Schizosaccharomyces / Q000502:physiology*; D029702:Schizosaccharomyces pombe Proteins / Q000737:chemistry / Q000378:metabolism*; D017384:Sequence Deletion / Q000235:genetics; D014335:Tropomyosin / Q000737:chemistry / Q000378:metabolism*
chemical_list: D000199:Actins; C410737:Cdc8 protein, S pombe; D018797:Cell Cycle Proteins; D029702:Schizosaccharomyces pombe Proteins; D014335:Tropomyosin; D000123:Acetyltransferases; D009218:Myosins
references.citation: Yeast. 1998 Jul;14(10):943-51
references.pmid: 9717240
references.citation: J Cell Sci. 2007 Dec 1;120(Pt 23):4093-8
references.pmid: 18003699
references.citation: Mol Biol Cell. 2005 Jan;16(1):358-71
references.pmid: 15537703
references.citation: J Muscle Res Cell Motil. 2001;22(1):5-49
references.pmid: 11563548
references.citation: J Cell Biol. 2000 Nov 13;151(4):789-800
references.pmid: 11076964
references.citation: J Biol Chem. 1994 Apr 8;269(14):10461-6
references.pmid: 8144630
references.citation: J Cell Sci. 2009 Dec 1;122(Pt 23):4330-40
references.pmid: 19887589
references.citation: J Biol Chem. 1994 Sep 30;269(39):24310-5
references.pmid: 7929088
references.citation: Curr Biol. 2005 Aug 23;15(16):1479-86
references.pmid: 16111942
references.citation: J Cell Sci. 2007 May 1;120(Pt 9):1635-45
references.pmid: 17452625
references.citation: Cell. 1989 Apr 21;57(2):233-42
references.pmid: 2649250
references.citation: J Cell Biol. 1997 Jun 16;137(6):1309-19
references.pmid: 9182664
references.citation: Methods Enzymol. 1991;194:795-823
references.pmid: 2005825
references.citation: Biochem J. 1991 Nov 1;279 ( Pt 3):711-8
references.pmid: 1953663
references.citation: Genetics. 1998 Jul;149(3):1265-75
references.pmid: 9649519
references.citation: J Cell Sci. 2009 Nov 1;122(Pt 21):3862-72
references.pmid: 19808886
references.citation: J Cell Biol. 1992 Jul;118(2):285-99
references.pmid: 1629236
references.citation: J Cell Biol. 2005 Aug 15;170(4):637-48
references.pmid: 16087707
references.citation: J Cell Sci. 2009 Nov 1;122(Pt 21):3856-61
references.pmid: 19808887
references.citation: J Muscle Res Cell Motil. 2008;29(6-8):213-9
references.pmid: 19116763
references.citation: J Mol Biol. 1995 Aug 11;251(2):191-6
references.pmid: 7643394
references.citation: J Cell Biol. 2004 Oct 25;167(2):315-25
references.pmid: 15504913
references.citation: Curr Biol. 2006 Jun 20;16(12):1161-70
references.pmid: 16782006
references.citation: Cell Motil Cytoskeleton. 2003 Mar;54(3):208-16
references.pmid: 12589679
references.citation: Bioessays. 1991 Sep;13(9):429-37
references.pmid: 1796905
references.citation: J Cell Sci. 2001 Jan;114(Pt 1):69-79
references.pmid: 11112691
references.citation: J Cell Biol. 2006 Dec 18;175(6):957-69
references.pmid: 17178912
references.citation: EMBO J. 1999 Feb 15;18(4):854-62
references.pmid: 10022828
references.citation: Nat Rev Mol Cell Biol. 2010 Feb;11(2):149-55
references.pmid: 20094054
references.citation: Genes Cells. 2002 Apr;7(4):375-84
references.pmid: 11952834
references.citation: Trends Cell Biol. 2005 Jun;15(6):333-41
references.pmid: 15953552
references.citation: Nature. 1994 Mar 3;368(6466):65-7
references.pmid: 8107884
references.citation: Traffic. 2008 Jun;9(6):936-50
references.pmid: 18346214
references.citation: Genetics. 2000 Oct;156(2):523-34
references.pmid: 11014803
references.citation: J Mol Biol. 2003 Jan 24;325(4):595-622
references.pmid: 12507466
references.citation: Mol Biol Cell. 2010 Mar 15;21(6):989-1000
references.pmid: 20110347
references.citation: Proc Natl Acad Sci U S A. 2001 Jul 17;98(15):8496-501
references.pmid: 11438684
references.citation: Yeast. 1994 Oct;10(10):1347-54
references.pmid: 7900424
references.citation: J Biol Chem. 1987 Jul 15;262(20):9730-5
references.pmid: 2954961
references.citation: Mol Biol Cell. 1997 Dec;8(12):2693-705
references.pmid: 9398685
references.citation: J Cell Biol. 1992 Aug;118(4):841-58
references.pmid: 1500427
references.citation: Biochemistry. 2006 Oct 24;45(42):12853-8
references.pmid: 17042503
references.citation: Proc Natl Acad Sci U S A. 2003 Jun 24;100(13):7644-9
references.pmid: 12808144
references.citation: J Cell Sci. 1988 Mar;89 ( Pt 3):343-57
references.pmid: 3198697
references.citation: Dev Cell. 2003 Nov;5(5):723-34
references.pmid: 14602073
references.citation: Nat Cell Biol. 2005 Sep;7(9):861-9
references.pmid: 16100513
references.citation: J Cell Biol. 1995 Feb;128(3):383-92
references.pmid: 7844152
references.citation: BMC Proc. 2009 Aug 04;3 Suppl 6:S2
references.pmid: 19660095
references.citation: Nat Cell Biol. 2001 Mar;3(3):235-44
references.pmid: 11231572
references.citation: Biophys J. 1993 Aug;65(2):693-701
references.pmid: 8218897
references.citation: J Biol Chem. 2003 Aug 15;278(33):30686-97
references.pmid: 12783868
references.citation: Nature. 1992 Nov 5;360(6399):84-7
references.pmid: 1436080