Quantitative influence of endogenous salicylic acid level on taxuyunnanine C biosynthesis in suspension cultures of Taxus chinensis.
Intracellular signals are critical to secondary metabolite biosynthesis by plant cells, but their quantitative effects are not yet well understood in plant cell cultures. Using Taxus chinensis suspension culture, which has the potential to provide a sustainable supply of highly useful bioactive taxoids, this work investigated the impact of endogenous salicylic acid (SA) level on taxuyunnanine C (Tc) biosynthesis. It was observed that the Tc accumulation was strongly dependent on the endogenous SA level. Under elicitation with an endogenous SA of 97.1 ± 11.8 µg/g dry weight, a maximal Tc content of 10.3 ± 0.52 mg/g dry weight was obtained. The transcription levels of Tc biosynthetic genes of geranylgeranyl diphosphate synthase and taxadiene synthase were also analyzed, and they were increased with increase of internal SA level. The results demonstrated that manipulation of endogenous SA level could be an efficient strategy for improving secondary metabolite biosynthesis in plant cell culture.
Zhou
Xin
X
State Key Laboratory of Bioreactor Engineering, School of Bioengineering, East China University of Science and Technology, Shanghai, China.
Zhong
Jian-Jiang
JJ
D000970:Antineoplastic Agents / Q000378:metabolism*; D001688:Biological Products / Q000378:metabolism; D053898:Biosynthetic Pathways / Q000235:genetics; D002478:Cells, Cultured; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D017343:Genes, Plant; D020156:Salicylic Acid / Q000378:metabolism*; D043823:Taxoids / Q000378:metabolism*; D020946:Taxus / Q000378:metabolism*
D000970:Antineoplastic Agents; D001688:Biological Products; D043823:Taxoids; C494600:taxuyunnanine C; D020156:Salicylic Acid