Coating of hydrophobins on three-dimensional electrospun poly(lactic-co-glycolic acid) scaffolds for cell adhesion.
Surface modification with hydrophobins is very important for cell adhesion in its applications in biosensor fabrication. In this study, we modified the surface of three-dimensional electrospun poly(lactide-co-glycolide) (PLGA) scaffolds with hydrophobin HFBI and collagen, and investigated its applications for cell adhesion. We found that HFBI could not only improve the hydrophilicity of the three-dimensional electrospun PLGA scaffolds but also endow the electrospun PLGA scaffolds with water permeability. This permeability should be attributed to both the hydrophilicity of the modified PLGA surface and the large positive capillary effect induced by the microstructures. Further experiment indicated that HFBI modification could improve collagen immobilization on the electrospun PLGA scaffolds and the HFBI/collagen modified electrospun PLGA scaffolds showed higher efficiency in promoting cell adhesion than the native PLGA scaffolds. This finding should be of potential application in biosensor device fabrication.
Hou
Sen
S
College of Life Science, Nankai University, Weijin Road 94, Tianjin, People's Republic of China.
Li
Xinxin
X
Li
Xiaoyu
X
Feng
Xizeng
X
D002448:Cell Adhesion / Q000187:drug effects*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003094:Collagen / Q000737:chemistry / Q000494:pharmacology; D005656:Fungal Proteins / Q000737:chemistry* / Q000494:pharmacology; D006801:Humans; D055767:Immobilized Proteins / Q000737:chemistry / Q000494:pharmacology; D019344:Lactic Acid / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D013601:T-Lymphocytes; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds; D017155:Wettability / Q000187:drug effects
D005656:Fungal Proteins; D055767:Immobilized Proteins; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D003094:Collagen