Detection of human cytomegalovirus in bronchoalveolar lavage fluid of lung transplant recipients reflects local virus replication and not contamination from the throat.
Whether bronchoalveolar lavage (BAL) fluid may be contaminated with oropharyngeal cytomegalovirus (CMV) has never been investigated. In an analysis of CMV DNA loads in 76 simultaneously obtained BAL fluid and throat wash samples from lung transplant recipients, we show that such contamination is unlikely and that detection of CMV DNA in BAL fluid reflects virus replication in the lung.
Kerschner
Heidrun
H
Department of Virology, Medical University of Vienna, Kinderspitalgasse 15, 1095 Vienna, Austria.
Jaksch
Peter
P
Zweytick
Barbara
B
Puchhammer-Stöckl
Elisabeth
E
D001992:Bronchoalveolar Lavage Fluid / Q000821:virology*; D003587:Cytomegalovirus / Q000302:isolation & purification* / Q000502:physiology; D003586:Cytomegalovirus Infections / Q000175:diagnosis* / Q000821:virology; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D006801:Humans; D016867:Immunocompromised Host; D016040:Lung Transplantation; D008297:Male; D010614:Pharynx / Q000821:virology; D014180:Transplantation; D019562:Viral Load; D014779:Virus Replication
D004279:DNA, Viral
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