Methylantcinate A induces tumor specific growth inhibition in oral cancer cells via Bax-mediated mitochondrial apoptotic pathway.
An ergostane type triterpenoid methylantcinate A (MAA) isolated from the fruiting bodies of Antrodia camphorata inhibited the growth of oral cancer cell lines OEC-M1 and OC-2 in a dose-dependent manner, without cytotoxic to normal oral gingival fibroblast cells. The major mechanism of growth inhibition was apoptosis induction, as shown by flow cytometric analysis of annexin V-FITC and propidium iodide staining, caspase-3 activation and DNA fragmentation. The increased expression of pro-apoptotic Bax, poly-(ADP-ribose) polymerase cleavage, and activated caspase-3 and decreased expression of anti-apoptotic Bcl-2 and Bcl-xL were also observed. These results provide the first evidence that the anti-oral cancer effects of MAA may involve a mechanism through the mitochondrial dependent pathway. Thus, results reported here may offer further impulse to the development of MAA analogues as potential chemotherapeutic targets for oral cancer complications.
Tsai
Wan-Chi
WC
School of Medical Laboratory and Biotechnology, Chung Shan Medical University, Taichung, Taiwan, ROC.
Rao
Yerra Koteswara
YK
Lin
Shih-Shen
SS
Chou
Ming-Yung
MY
Shen
Yi-Ting
YT
Wu
Chih-Hao
CH
Geethangili
Madamanchi
M
Yang
Chi-Chiang
CC
Tzeng
Yew-Min
YM
D000970:Antineoplastic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D055491:Antrodia / Q000737:chemistry*; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D002460:Cell Line; D045744:Cell Line, Tumor; D005347:Fibroblasts / Q000187:drug effects; D048690:Fruiting Bodies, Fungal / Q000737:chemistry; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D008928:Mitochondria / Q000187:drug effects; D009062:Mouth Neoplasms / Q000188:drug therapy*; D014315:Triterpenes / Q000302:isolation & purification / Q000494:pharmacology*; D015854:Up-Regulation / Q000187:drug effects; D051028:bcl-2-Associated X Protein / Q000235:genetics / Q000378:metabolism*
D000970:Antineoplastic Agents; D014315:Triterpenes; D051028:bcl-2-Associated X Protein; C553655:methyl antcinate A; D053148:Caspase 3