title: Molecular cloning of IKKβ from the mandarin fish Siniperca chuatsi and its up-regulation in cells by ISKNV infection.
abstract: The IκB kinase β (IKKβ) plays crucial roles in regulating activation of nuclear factor-kappa B (NF-κB) in response to proinflammatory factors and microbial and viral infections. Here, we report the cloning of an IKKβ cDNA (named SicIKKβ) from the mandarin fish Siniperca chuatsi. The full-length cDNA is 4052bp and contains an ORF that encodes a predicted protein of 743-amino acid residues. The deduced amino acid sequence of SicIKKβ has the same domain organization as human IKKβ, which consists of a serine/threonine kinase domain, a leucine zipper motif and a putative helix-loop-helix motif. Quantitative RT-PCR showed that SicIKKβ was ubiquitously expressed in tissues of mandarin fish, and its expression in mandarin fish fry (MFF-1) cells was up-regulated during the course of ISKNV infection.
authors.affiliation: State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, PR China.
authors.forename: Wei-Jian
authors.initials: WJ
authors.lastname: Chen
authors.forename: Chang-Jun
authors.initials: CJ
authors.lastname: Guo
authors.forename: Zhi-Cheng
authors.initials: ZC
authors.lastname: Zhou
authors.forename: Li-Qun
authors.initials: LQ
authors.lastname: Yuan
authors.forename: Zhi-Ming
authors.initials: ZM
authors.lastname: Xiang
authors.forename: Shao-Ping
authors.initials: SP
authors.lastname: Weng
authors.forename: Ying-Fen
authors.initials: YF
authors.lastname: Zhang
authors.forename: Xiao-Qing
authors.initials: XQ
authors.lastname: Yu
authors.forename: Jian-Guo
authors.initials: JG
authors.lastname: He
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D004266:DNA Virus Infections / Q000201:enzymology / Q000235:genetics / Q000276:immunology / Q000662:veterinary*; D005393:Fish Diseases / Q000201:enzymology* / Q000235:genetics / Q000276:immunology; D051550:I-kappa B Kinase / Q000235:genetics*; D007497:Iridoviridae / Q000276:immunology; D016328:NF-kappa B / Q000378:metabolism; D010473:Perciformes / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D017422:Sequence Analysis, DNA / Q000662:veterinary; D015854:Up-Regulation
chemical_list: D016328:NF-kappa B; D051550:I-kappa B Kinase