Immunologic properties of human dermal fibroblasts.
Human dermal fibroblasts are known not to express human leukocyte antigen (HLA)-DR message or protein in the absence of interferon (IFN)-γ. To use allogeneic dermal fibroblasts for cell therapy, as a revalidation, the cells at passage 12 were analyzed for HLA-DR mRNA and surface protein. Although no significant HLA-DR surface protein was found, HLA-DR mRNA expression was observed, without interferon-γ. At an early passage, although HLA-DR surface protein was not found, prominent expression of HLA-DR mRNA was observed without IFN-γ stimulation, which was not typical of dermal fibroblasts studied so far. Intracytoplasmic HLA-DR protein was also not detected, which suggests that the mRNA was not translated. There was no marked stimulation of T-cell proliferation by the fibroblasts in the absence or presence of IFN-γ. Interestingly, indoleamine dioxygenase, a molecule involved in immunosuppression, was also expressed in dermal fibroblasts in the absence of IFN-γ.
Deshpande
Manisha
M
Reliance Life Sciences Pvt Ltd., Dhirubhai Ambani Life Sciences Centre, Maharashtra, India. manisha_deshpande@relbio.com
Tipnis
Shabari
S
Shetty
Prathibha
P
Ghosh
Deepa
D
Viswanathan
Chandra
C
Majumdar
Anish Sen
AS
D049109:Cell Proliferation; D002478:Cells, Cultured; D020405:Dermis / Q000473:pathology*; D005347:Fibroblasts / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D052816:Foreskin / Q000473:pathology; D006684:HLA-DR Antigens / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D007108:Immune Tolerance; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase / Q000378:metabolism; D007231:Infant, Newborn; D007371:Interferon-gamma / Q000276:immunology / Q000378:metabolism; D008213:Lymphocyte Activation; D008297:Male; D012333:RNA, Messenger / Q000032:analysis*; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism* / Q000473:pathology
D006684:HLA-DR Antigens; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase; D012333:RNA, Messenger; D007371:Interferon-gamma