Measuring bacterial growth by refractive index tapered fiber optic biosensor.
A single-mode tapered fiber optic biosensor was utilized for real-time monitoring of the Escherichia coli (E. coli K-12) growth in an aqueous medium. The applied fiber tapers were fabricated using heat-pulling method with waist diameter and length of 6-7μm and 3mm, respectively. The bacteria were immobilized on the tapered surface using Poly-l-Lysine. By providing the proper condition, bacterial population growth on the tapered surface increases the average surface density of the cells and consequently the refractive index (RI) of the tapered region would increase. The adsorption of the cells on the tapered fiber leads to changes in the optical characteristics of the taper. This affects the evanescent field leading to changes in optical throughput. The bacterial growth rate was monitored at room temperature by transmission of a 1558.17nm distributed feedback (DFB) laser through the tapered fiber. At the same condition, after determining the growth rate of E. coli by means of colony counting method, we compared the results with that obtained from the fiber sensor measurements. This novel sensing method, promises new application such as rapid analysis of the presence of bacteria.
Zibaii
Mohammad Ismail
MI
Shahid Beheshti University, Evin, Tehran, Iran.
Kazemi
Alireza
A
Latifi
Hamid
H
Azar
Mahmoud Karimi
MK
Hosseini
Seyed Masoud
SM
Ghezelaiagh
Mohammad Hossein
MH
D015374:Biosensing Techniques / Q000379:methods*; D018914:Cells, Immobilized; D004926:Escherichia coli / Q000254:growth & development* / Q000528:radiation effects; D005336:Fiber Optic Technology / Q000379:methods; D006863:Hydrogen-Ion Concentration; D007834:Lasers; D010100:Oxygen / Q000494:pharmacology; D011107:Polylysine / Q000737:chemistry; D012031:Refractometry / Q000379:methods
D011107:Polylysine; D010100:Oxygen