Feasibility of the T-SPOT.TB assay for the immunodiagnosis of smear-positive pulmonary tuberculosis using induced sputum.
Diagnosis of active tuberculosis (TB) remains a challenge. In this a preliminary proof-of-concept study seeking to determine the feasibility of using cells from induced sputum for the immunodiagnosis of pulmonary TB (PTB) in smear-positive cases, a total of 75 subjects with PTB (n = 31) and healthy controls (n = 44) underwent the blood T-SPOT(R).TB test. T-SPOT.TB in induced sputum samples was performed in 29 of 31 TB and 14/44 healthy subjects. Induced sputum T-SPOT.TB results were indeterminate in 72.6% of TB and 100% of healthy subjects. The T-SPOT.TB test in induced sputum samples does not seem a feasible method for diagnosing PTB and needs improvement.
Dilektasli
A G
AG
Department of Pulmonary Diseases, Baskent University School of Medicine, Ankara, Turkey. asligorekd@gmail.com
Erdem
E
E
Durukan
E
E
Eyüboğlu
F O
FO
D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D006801:Humans; D007118:Immunoassay / Q000379:methods; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000276:immunology / Q000302:isolation & purification*; D013183:Sputum / Q000276:immunology / Q000382:microbiology*; D013601:T-Lymphocytes / Q000276:immunology; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis*; D055815:Young Adult