Development of nanoparticle probes for multiplex SERS imaging of cell surface proteins.
Multiplexed SERS imaging of receptor proteins on the surface of mammalian cells has been carried out using functionalized silver nanoparticles. Deconvolution of four differently functionalized nanoparticles is readily achieved, and using this approach, receptor co-localization can be probed and protein-protein interactions can be elucidated at the surface of cells.
Kennedy
David C
DC
Steacie Institute for Molecular Sciences, National Research Council Canada, 100 Sussex Drive, Ottawa, K1A 0R6, Canada.
Hoop
Kelly A
KA
Tay
Li-Lin
LL
Pezacki
John Paul
JP
D000818:Animals; D051244:Caveolin 3 / Q000737:chemistry; D008024:Ligands; D008565:Membrane Proteins / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D018613:Microscopy, Confocal; D032383:Myocytes, Cardiac / Q000166:cytology; D051381:Rats; D018343:Receptors, Adrenergic, beta-2 / Q000737:chemistry; D012834:Silver / Q000737:chemistry; D013059:Spectrum Analysis, Raman
D051244:Caveolin 3; D008024:Ligands; D008565:Membrane Proteins; D018343:Receptors, Adrenergic, beta-2; D012834:Silver