Synthesis of biologically active biotinylated muramyl dipeptides.
Muramyl dipeptide (MDP) is believed to interact with an innate immune receptor, Nod2. To identify the cellular receptor for MDP, we have synthesized biotinylated MDP isomers and tested the ability of these compounds to activate Nod2 in a cell-based assay. We found that the modification of MDP does not perturb its ability to activate Nod2. These tagged versions of MDP will be useful to identify the cellular receptor of the immunostimulatory molecules.
Grimes
Catherine Leimkuhler
CL
Harvard University, Department of Molecular and Cellular Biology, Faculty of Arts and Sciences, Cambridge, MA 02138, USA.
Podolsky
Daniel K
DK
O'Shea
Erin K
EK
D000119:Acetylmuramyl-Alanyl-Isoglutamine / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D000276:Adjuvants, Immunologic / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D019921:Biotinylation; D002460:Cell Line; D006801:Humans; D007113:Immunity, Innate / Q000187:drug effects; D007536:Isomerism; D053473:Nod2 Signaling Adaptor Protein / Q000276:immunology*
D000276:Adjuvants, Immunologic; D053473:Nod2 Signaling Adaptor Protein; D000119:Acetylmuramyl-Alanyl-Isoglutamine
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