Castration- and aging-induced changes in the expression of zinc transporter and metallothionein in rat prostate.
Prostate tissue contains high concentrations of zinc. Zinc content in the prostate gland changes in prostatic disease, such as benign prostate hyperplasia and prostate cancer, which occur more frequently with increasing age. Prostate zinc content is also known to decrease after castration in animal models. It is not clear how prostate zinc content is regulated; therefore, to clarify the mechanisms underlying zinc homeostasis, we examined zinc content and the expression of zinc transporters and metallothioneins in the prostates of aged or castrated rats. Zinc concentration was measured by flame atomic absorption spectrometry. The mRNA expression of zinc transporters and metallothioneins was determined by real-time reverse transcriptase polymerase chain reaction analysis. The expression of the zinc transporter Slc30a2 (Znt2) in ventral prostate (VP) of aged rats (21 months) was approximately 21-fold higher than that in VP of young rats (4 months), and zinc levels in VP of young rats increased significantly compared with that in aged rats. Zinc content in lateral prostate (LP) and dorsal prostate did not differ between young and aged rats. Decreased metallothionein-3 (Mt3) expression was observed in LP of castrated rats, and this reduction was prevented by testosterone replacement. Zinc content and Mt3 expression levels correlated significantly in rat LP. Our findings suggest that Mt3 could play a critical role in zinc homeostasis in rat LP.
Iguchi
Kazuhiro
K
Laboratory of Pharmaceutics, Gifu Pharmaceutical University, 1-25-4 Daigaku-nishi, Gifu 501-1196, Japan.
Morihara
Naoaki
N
Usui
Shigeyuki
S
Hayama
Minoru
M
Sugimura
Yoshiki
Y
Hirano
Kazuyuki
K
D000375:Aging / Q000502:physiology*; D000818:Animals; D027682:Cation Transport Proteins / Q000096:biosynthesis*; D008297:Male; D008668:Metallothionein / Q000096:biosynthesis*; D000090023:Metallothionein 3; D009419:Nerve Tissue Proteins / Q000096:biosynthesis; D009919:Orchiectomy; D011467:Prostate / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015032:Zinc / Q000378:metabolism*
D027682:Cation Transport Proteins; D000090023:Metallothionein 3; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; C099189:Slc30a2 protein, rat; D008668:Metallothionein; D015032:Zinc