title: Non-contact fluorescent bleaching-independent method for temperature measurement in microfluidic systems based on DNA melting curves.
abstract: This report introduces a bleaching-independent temperature measurement method based on the analysis of the fluorescence emitted during the melting of DNA molecules with the SYBR-Green I intercalator, in a microvolume where the strong non-linearity of the signal is used to eliminate the photobleaching effect as well as to determine the heat transfer rate between a heater and the sample and the temperature non-uniformity within the sample.
authors.affiliation: Institute of Microelectronics, 11 Science Park Road, Singapore. pavel@kist-europe.de
authors.forename: Pavel
authors.initials: P
authors.lastname: Neuzil
authors.forename: Fang
authors.initials: F
authors.lastname: Cheng
authors.forename: Jeffrey Bo Woon
authors.initials: JB
authors.lastname: Soon
authors.forename: Lan Liu
authors.initials: LL
authors.lastname: Qian
authors.forename: Julien
authors.initials: J
authors.lastname: Reboud
mesh_terms: D004247:DNA / Q000737:chemistry*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D008856:Microscopy, Fluorescence / Q000295:instrumentation*; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation*; D013821:Thermometers; D044366:Transition Temperature
chemical_list: D004247:DNA