Crystallization and preliminary X-ray diffraction analysis of rat autotaxin.
Rat autotaxin has been cloned, expressed, purified to homogeneity and crystallized via hanging-drop vapour diffusion using PEG 3350 as precipitant and ammonium iodide and sodium thiocyanate as salts. The crystals diffracted to a maximum resolution of 2.05 A and belonged to space group P1, with unit-cell parameters a=53.8, b=63.3, c=70.5 A, alpha=98.8, beta=106.2, gamma=99.8 degrees. Preliminary X-ray diffraction analysis indicated the presence of one molecule per asymmetric unit, with a solvent content of 47%.
Day
Jacqueline E
JE
Pfizer Global Research and Development, St Louis Laboratories, 700 Chesterfield Parkway West, Chesterfield, MO 63017, USA.
Hall
Troii
T
Pegg
Lyle E
LE
Benson
Timothy E
TE
Hausmann
Jens
J
Kamtekar
Satwik
S
D000818:Animals; D003460:Crystallization; D018360:Crystallography, X-Ray; D010727:Phosphoric Diester Hydrolases / Q000737:chemistry*; D011755:Pyrophosphatases / Q000737:chemistry*; D051381:Rats; D000099189:Lysophospholipase D
D010727:Phosphoric Diester Hydrolases; D011755:Pyrophosphatases; D000099189:Lysophospholipase D
Methods Enzymol. 1997;276:307-26
27754618
Bioorg Med Chem. 2008 Feb 15;16(4):1784-95
18036821
J Biol Chem. 2007 Apr 13;282(15):11084-91
17307740
Bioorg Med Chem. 2008 Mar 1;16(5):2212-25
18082408
Biochim Biophys Acta. 2008 Sep;1781(9):525-30
18485925
Clin Exp Immunol. 2001 Jan;123(1):147-54
11168012
Cancer Lett. 2008 Aug 8;266(2):203-8
18378389
J Pharmacol Exp Ther. 2008 Dec;327(3):809-19
18755937
Mol Cancer Ther. 2008 Oct;7(10):3352-62
18852138
Nat Immunol. 2008 Apr;9(4):415-23
18327261
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Sep 1;66(Pt 9):1130-5
20823545
Biochim Biophys Acta. 2008 Sep;1781(9):588-94
18454946
Biochemistry. 2006 Aug 15;45(32):9788-803
16893180
J Appl Crystallogr. 2007 Aug 1;40(Pt 4):658-674
19461840
J Mol Biol. 1968 Apr 28;33(2):491-7
5700707
Mol Pain. 2008 Feb 08;4:6
18261210
Exp Cell Res. 2008 Feb 1;314(3):530-42
18054784
J Cell Biol. 2002 Jul 22;158(2):227-33
12119361