A processed multidomain mycoplasma hyopneumoniae adhesin binds fibronectin, plasminogen, and swine respiratory cilia.
Porcine enzootic pneumonia is a chronic respiratory disease that affects swine. The etiological agent of the disease, Mycoplasma hyopneumoniae, is a bacterium that adheres to cilia of the swine respiratory tract, resulting in loss of cilia and epithelial cell damage. A M. hyopneumoniae protein P116, encoded by mhp108, was investigated as a potential adhesin. Examination of P116 expression using proteomic analyses observed P116 as a full-length protein and also as fragments, ranging from 17 to 70 kDa in size. A variety of pathogenic bacterial species have been shown to bind the extracellular matrix component fibronectin as an adherence mechanism. M. hyopneumoniae cells were found to bind fibronectin in a dose-dependent and saturable manner. Surface plasmon resonance was used to show that a recombinant C-terminal domain of P116 bound fibronectin at physiologically relevant concentrations (K(D) 24 ± 6 nm). Plasmin(ogen)-binding proteins are also expressed by many bacterial pathogens, facilitating extracellular matrix degradation. M. hyopneumoniae cells were found to also bind plasminogen in a dose-dependent and saturable manner; the C-terminal domain of P116 binds to plasminogen (K(D) 44 ± 5 nm). Plasminogen binding was abolished when the C-terminal lysine of P116 was deleted, implicating this residue as part of the plasminogen binding site. P116 fragments adhere to the PK15 porcine kidney epithelial-like cell line and swine respiratory cilia. Collectively these data suggest that P116 is an important adhesin and virulence factor of M. hyopneumoniae.
Seymour
Lisa M
LM
Schools of Biological Sciences, University of Wollongong, New South Wales 2522, Australia.
Deutscher
Ania T
AT
Jenkins
Cheryl
C
Kuit
Tracey A
TA
Falconer
Linda
L
Minion
F Chris
FC
Crossett
Ben
B
Padula
Matthew
M
Dixon
Nicholas E
NE
Djordjevic
Steven P
SP
Walker
Mark J
MJ
D018829:Adhesins, Bacterial / Q000737:chemistry*; D000818:Animals; D002923:Cilia / Q000378:metabolism*; D005353:Fibronectins / Q000737:chemistry*; D008168:Lung / Q000382:microbiology; D008863:Microspheres; D045705:Mycoplasma hyopneumoniae / Q000378:metabolism*; D010957:Plasmids / Q000378:metabolism; D010958:Plasminogen / Q000737:chemistry*; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D040901:Proteomics / Q000379:methods; D013552:Swine
D018829:Adhesins, Bacterial; D005353:Fibronectins; D010958:Plasminogen
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