Overexpression of LYRM1 induces mitochondrial impairment in 3T3-L1 adipocytes.
Homo sapiens LYR motif containing 1 (LYRM1) is a recently discovered gene involved in adipose tissue homeostasis and obesity-associated insulin resistance. The exact mechanism by which LYRM1 induces insulin resistance has not yet been fully elucidated. In this study, we demonstrated that the overexpression of LYRM1 in 3T3-L1 adipocytes resulted in reduced insulin-stimulated glucose uptake, an abnormal mitochondrial morphology, and a decrease in intracellular ATP synthesis and mitochondrial membrane potential. In addition, LYRM1 overexpression led to excessive production of intracellular of reactive oxygen species. Collectively, our results indicated that the overexpression of LYRM1 caused mitochondrial dysfunction in adipocytes, which might be responsible for the development of LYRM1-induced insulin resistance.
Cao
Xin-Guo
XG
Nanjing Maternal and Child Health Hospital of Nanjing Medical University, Nanjing 210004, China.
Kou
Chun-Zhao
CZ
Zhao
Ya-Ping
YP
Gao
Chun-Lin
CL
Zhu
Chun
C
Zhang
Chun-Mei
CM
Ji
Chen-Bo
CB
Qin
Da-Ni
DN
Zhang
Min
M
Guo
Xi-Rong
XR
D041721:3T3-L1 Cells; D000255:Adenosine Triphosphate / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D000818:Animals; D051017:Apoptosis Regulatory Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D002478:Cells, Cultured; D004272:DNA, Mitochondrial / Q000235:genetics / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006706:Homeostasis / Q000187:drug effects; D007328:Insulin / Q000378:metabolism / Q000494:pharmacology; D007333:Insulin Resistance; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D051379:Mice; D008928:Mitochondria / Q000378:metabolism* / Q000473:pathology; D017382:Reactive Oxygen Species / Q000378:metabolism
D051017:Apoptosis Regulatory Proteins; D004272:DNA, Mitochondrial; D007328:Insulin; C538836:LYRM1 protein, human; D017382:Reactive Oxygen Species; D000255:Adenosine Triphosphate; D005947:Glucose