In vitro capturing of various lipophilic illicit drugs by lipid dispersions. An electrokinetic capillary chromatography and fluorescence polarization study.
Fatal drug overdoses are a cause for concern all over the world. We present here a lipid-based formulation which has a strong affinity for some common illicit street drugs and can be used in vivo as a lipid 'sink'. In this study, the in vitro interactions of nine lipophilic drugs and three lipid dispersions were determined by electrokinetic capillary chromatography and fluorescence polarization. Two lipid dispersions, zwitterionic 1-palmitoyl-2-oleyl-sn-glycero-3-phosphocholine (POPC) and an anionic mixture of POPC and 1-palmitoyl-2-oleyl-sn-glycero-3-[phospho-rac-(1-glycerol)] (POPG) were tested and compared with a commercial lipid dispersion Intralipid(®), which has been successfully used for resuscitation of patients in cases of anesthetic overdoses. The interactions between dispersions and the drugs were quantified by means of retention factors and distribution constants, which makes the results highly comparable to those obtained from any other formulation of lipids. The results demonstrate a stronger interaction between the drugs and an artificial liposome dispersion than with the commercial Intralipid dispersion. The liposome dispersion composed of POPC and POPG functions as a lipid 'sink' for efficient entrapment of various lipophilic drugs.
Lokajová
Jana
J
Laboratory of Analytical Chemistry, Department of Chemistry, 00014 University of Helsinki, Finland.
Pukkila
Jari
J
Holopainen
Juha M
JM
Wiedmer
Susanne K
SK
D002845:Chromatography / Q000379:methods*; D055664:Electrochemical Techniques; D005454:Fluorescence Polarization / Q000379:methods*; D013287:Illicit Drugs / Q000737:chemistry*; D008055:Lipids / Q000737:chemistry*; D008081:Liposomes; D015394:Molecular Structure; D015813:Substance Abuse Detection / Q000379:methods*
D013287:Illicit Drugs; D008055:Lipids; D008081:Liposomes