Studies on MAP Kinase signaling in the immune system.
The primary function of the immune system is to protect the organism from invading pathogens. In response to pathogen invasion, multiple signaling pathways are activated in immune cells, leading to diverse immune defense mechanisms. Chief among these pathways is the activation of MAPKs, which are crucial for transcriptional and nontranscriptional responses of the immune system. Here we describe protocols to study the roles of MAPKs in T lymphocytes, a cell type central for immune regulation. Specifically, we describe flow cytometry-based assays to analyze the roles of MAPKs in the development, homeostasis, proliferation, and apoptosis of murine T cells. We also describe methods to examine the activation of MAPKs in T cells.
Chi
Hongbo
H
Department of Immunology, St Jude Children's Research Hospital, Memphis, TN, USA. hongbo.chi@stjude.org
Flavell
Richard A
RA
D000818:Animals; D017304:Annexin A5 / Q000378:metabolism; D017209:Apoptosis; D015704:CD4 Antigens / Q000378:metabolism; D049109:Cell Proliferation; D002469:Cell Separation; D005434:Flow Cytometry; D005452:Fluoresceins / Q000378:metabolism; D006706:Homeostasis; D007107:Immune System / Q000166:cytology* / Q000201:enzymology; D020935:MAP Kinase Signaling System; D051379:Mice; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D010750:Phosphoproteins / Q000378:metabolism; D013194:Staining and Labeling; D013388:Succinimides / Q000378:metabolism; D013601:T-Lymphocytes / Q000166:cytology / Q000378:metabolism
C087165:5-(6)-carboxyfluorescein diacetate succinimidyl ester; D017304:Annexin A5; D015704:CD4 Antigens; D005452:Fluoresceins; D010750:Phosphoproteins; D013388:Succinimides; D020928:Mitogen-Activated Protein Kinases
Immunity. 2005 Oct;23(4):431-43
16226508
Nat Immunol. 2006 Aug;7(8):851-8
16799562
Physiol Rev. 2001 Apr;81(2):807-69
11274345
Cell. 2006 Feb 24;124(4):783-801
16497588
Annu Rev Immunol. 2002;20:55-72
11861597