HEMA enhances IgG1 production by human B-cells in vitro.
We have previously shown that the resin monomer 2-hydroxyethylmethacrylate (HEMA) affects mouse B-lymphocyte activity, leading to increased IgG1 antibody production in vivo. In the present study, we tested, in vitro, the hypothesis that HEMA also affects human B-lymphocyte activity. The in vitro production of IgG1, IgM, and IgA in supernatants from purified human CD19+ B-lymphocyte cultures, containing different concentrations of HEMA, was assayed with ELISA. Proliferation was measured by [methyl-(3)H] thymidine incorporation. Of the different HEMA concentrations used, the lower concentrations caused a significant increase in IgG1 production, but not in IgM or IgA production, in vitro. The lower HEMA concentrations did not significantly change B-cell proliferation. At the highest concentration, HEMA significantly suppressed IgG1 and IgM production, as well as B-cell proliferation, in vitro. In conclusion, HEMA can, at certain concentrations, selectively enhance human B-lymphocyte IgG1 production.


BACKGROUND
2-hydroxyethylmethacrylate (HEMA), Dulbecco's Modified Eagle's Medium supplemented with heat-inactivated fetal bovine serum, gentamycin, penicillin, and streptomycin (D-MEM++++), Enzyme-linked Immuno-sorbent Assay (ELISA), phosphate-buffered saline (PBS), ovalbumin (OVA), pokeweed mitogen (PWM), counts per minute (CPM).
Andersson
J
J
University of Gothenburg, Department of Odontology, Section for Oral Microbiology and Oral Immunology, Medicinaregatan 12G, S41390 Gothenburg, Sweden. jennie.andersson@odontologi.gu.se
Dahlgren
U
U
D018941:Antigens, CD19 / Q000276:immunology; D001402:B-Lymphocytes / Q000145:classification / Q000187:drug effects* / Q000276:immunology; D018929:Cell Culture Techniques; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003764:Dental Materials / Q000494:pharmacology*; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis / Q000187:drug effects; D007074:Immunoglobulin G / Q000032:analysis / Q000187:drug effects*; D007075:Immunoglobulin M / Q000032:analysis / Q000187:drug effects; D008422:Materials Testing; D008689:Methacrylates / Q000494:pharmacology*; D019275:Radiopharmaceuticals; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D018941:Antigens, CD19; D003764:Dental Materials; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D008689:Methacrylates; D019275:Radiopharmaceuticals; D014316:Tritium; C005044:hydroxyethyl methacrylate; D013936:Thymidine