QTL mapping of internal heat necrosis in tetraploid potato.
Internal heat necrosis (IHN) is a physiological disorder of potato tubers. We developed a linkage map of tetraploid potato using AFLP and SSR markers, and mapped QTL for mean severity and percent incidence of IHN. Phenotypic data indicated that the distribution of IHN is skewed toward resistance. Late foliage maturity was slightly but significantly correlated with increased IHN symptoms. The linkage map for 'Atlantic', the IHN-susceptible parent, covered 1034.4 cM and included 13 linkage groups, and the map for B1829-5, the IHN-resistant parent, covered 940.2 cM and contained 14 linkage groups. QTL for increased resistance to IHN were located on chromosomes IV, V, and groups VII and X of 'Atlantic', and on group VII of B1829-5 in at least 2 of 3 years. The QTL explained between 4.5 and 29.4% of the variation for mean severity, and from 3.7 to 14.5% of the variation for percent incidence. Most QTL detected were dominant, and associated with decreased IHN symptoms. One SSR and 13 AFLP markers that were linked to IHN were tested in a second population. One AFLP marker was associated with decreased symptoms in both populations. The SSR marker was not associated with IHN in the second population, but was closely linked in repulsion to another marker that was associated with IHN, and had the same (negative) effect on the trait as the SSR marker did in the first population. The correlation between maturity and IHN may be partially explained by the presence of markers on chromosome V that are linked to both traits. This research represents the first molecular genetic research of IHN in potato.
McCord
P H
PH
Vegetable and Forage Crop Research Unit, USDA, ARS, 24106 N. Bunn Road, Prosser, WA 99350, USA.
Sosinski
B R
BR
Haynes
K G
KG
Clough
M E
ME
Yencho
G C
GC
D000383:Agriculture; D002874:Chromosome Mapping / Q000379:methods*; D008040:Genetic Linkage; D005819:Genetic Markers; D006358:Hot Temperature; D009336:Necrosis; D010935:Plant Diseases / Q000235:genetics*; D040641:Quantitative Trait Loci / Q000235:genetics*; D019655:Quantitative Trait, Heritable; D011198:Solanum tuberosum / Q000235:genetics*; D057891:Tetraploidy
D005819:Genetic Markers
Nucleic Acids Res. 1995 Nov 11;23(21):4407-14
7501463
BMC Plant Biol. 2003 Dec 19;3:9
14683527
Genetics. 2006 Jun;173(2):1075-87
16582432
Theor Appl Genet. 2004 Aug;109(4):733-9
15146319
Theor Appl Genet. 2003 Apr;106(6):1107-15
12671760
Mol Genet Genomics. 2001 May;265(3):497-507
11405633
Theor Appl Genet. 2008 Jan;116(2):193-211
17938877
Nucleic Acids Res. 1994 Sep;22(17):3640-59
7937074
Theor Appl Genet. 2004 Feb;108(4):603-11
14614564
J Hered. 2002 Jan-Feb;93(1):77-8
12011185
J Hered. 2007 Nov-Dec;98(7):727-9
17965198
Nat Biotechnol. 2000 Feb;18(2):233-4
10657137
Theor Appl Genet. 2006 Dec;114(1):131-42
17082950
Theor Appl Genet. 2005 Jul;111(2):291-312
15926074
J Agric Res. 1945 Dec 1;71:487-505
21004241
Genetics. 2004 Oct;168(2):983-95
15514069
Proc Natl Acad Sci U S A. 2004 May 4;101(18):7040-5
15100415
Bioinformatics. 2008 Dec 1;24(23):2788-9
18940826
Proc Natl Acad Sci U S A. 1995 Aug 15;92(17):7719-23
7644483
Genetics. 1994 Nov;138(3):963-71
7851788
Genetics. 2001 Dec;159(4):1819-32
11779817
Theor Appl Genet. 2005 Aug;111(3):456-66
15942755