title: Emerging complex pathways of the actomyosin powerstroke.
abstract: Actomyosin powers muscle contraction and various cellular activities, including cell division, differentiation, intracellular transport and sensory functions. Despite their crucial roles, key aspects of force generation have remained elusive. To perform efficient force generation, the powerstroke must occur while myosin is bound to actin. Paradoxically, this process must be initiated when myosin is in a very low actin-affinity state. Recent results shed light on a kinetic pathway selection mechanism whereby the actin-induced activation of the swing of myosin's lever enables efficient mechanical functioning. Structural elements and biochemical principles involved in this mechanism are conserved among various NTPase-effector (e.g. kinesin-microtubule, G protein exchange factor and kinase-scaffold protein) systems that perform chemomechanical or signal transduction.
authors.affiliation: Department of Biochemistry, Eötvös University, Pázmány P. stny. 1/C, H-1117 Budapest, Hungary. malna@elte.hu
authors.forename: András
authors.initials: A
authors.lastname: Málnási-Csizmadia
authors.forename: Mihály
authors.initials: M
authors.lastname: Kovács
mesh_terms: D000205:Actomyosin / Q000737:chemistry / Q000378:metabolism*; D000818:Animals; D006801:Humans; D009119:Muscle Contraction; D009711:Nucleotides / Q000378:metabolism
chemical_list: D009711:Nucleotides; D000205:Actomyosin
references.citation: Biochemistry. 1971 Dec 7;10(25):4617-24
references.pmid: 4258719
references.citation: EMBO J. 2007 Jan 10;26(1):265-74
references.pmid: 17213877
references.citation: Structure. 2005 Jan;13(1):131-41
references.pmid: 15642268
references.citation: Philos Trans R Soc Lond B Biol Sci. 2004 Dec 29;359(1452):1867-77
references.pmid: 15647162
references.citation: J Physiol. 2005 Mar 15;563(Pt 3):671-87
references.pmid: 15611023
references.citation: J Biol Chem. 2002 Jul 5;277(27):24114-9
references.pmid: 11959853
references.citation: J Biol Chem. 2008 Mar 28;283(13):8153-63
references.pmid: 18211892
references.citation: Nature. 1990 Sep 6;347(6288):37-44
references.pmid: 2395459
references.citation: Genome Biol. 2007;8(9):R196
references.pmid: 17877792
references.citation: Philos Trans R Soc Lond B Biol Sci. 2004 Dec 29;359(1452):1819-28
references.pmid: 15647158
references.citation: Annu Rev Biophys. 2010;39:539-57
references.pmid: 20192767
references.citation: Curr Opin Cell Biol. 2004 Feb;16(1):61-7
references.pmid: 15037306
references.citation: Science. 1993 Jul 2;261(5117):50-8
references.pmid: 8316857
references.citation: Nature. 1954 May 22;173(4412):971-3
references.pmid: 13165697
references.citation: Nature. 1954 May 22;173(4412):973-6
references.pmid: 13165698
references.citation: Biophys J. 1991 Feb;59(2):329-42
references.pmid: 2009356
references.citation: J Cell Biol. 1996 Oct;135(2):291-302
references.pmid: 8896589
references.citation: Trends Biochem Sci. 2000 Dec;25(12):607-11
references.pmid: 11116187
references.citation: Proc Natl Acad Sci U S A. 2010 Apr 13;107(15):6799-804
references.pmid: 20351242
references.citation: Proc Natl Acad Sci U S A. 1995 Nov 7;92(23):10482-6
references.pmid: 7479824
references.citation: J Biol Chem. 2000 Dec 8;275(49):38494-9
references.pmid: 10954715
references.citation: Mol Cell. 2007 Jan 12;25(1):141-9
references.pmid: 17218277
references.citation: Proc Natl Acad Sci U S A. 2007 Jun 12;104(24):9994-9
references.pmid: 17548820
references.citation: Structure. 2007 May;15(5):553-64
references.pmid: 17502101
references.citation: Adv Protein Chem. 2005;71:161-93
references.pmid: 16230112
references.citation: Proc Natl Acad Sci U S A. 2000 Oct 10;97(21):11238-43
references.pmid: 11016966
references.citation: Science. 2006 Feb 10;311(5762):822-6
references.pmid: 16424299
references.citation: Biochemistry. 1995 Jul 18;34(28):8960-72
references.pmid: 7619795
references.citation: J Biol Chem. 2008 Dec 5;283(49):34121-8
references.pmid: 18854311
references.citation: Nat Cell Biol. 2003 Nov;5(11):980-6
references.pmid: 14578909
references.citation: Biophys J. 2005 Nov;89(5):L34-6
references.pmid: 16169988
references.citation: Nature. 2003 Sep 25;425(6956):419-23
references.pmid: 14508494
references.citation: Biochem J. 1974 Aug;141(2):331-49
references.pmid: 4281653
references.citation: J Muscle Res Cell Motil. 1998 Nov;19(8):877-86
references.pmid: 10047987
references.citation: Biochem J. 1974 Aug;141(2):351-64
references.pmid: 4281654
references.citation: EMBO J. 2004 Nov 24;23(23):4527-37
references.pmid: 15510214
references.citation: Proc Natl Acad Sci U S A. 2009 Apr 14;106(15):6140-5
references.pmid: 19325123
references.citation: Biophys J. 2009 May 6;96(9):3673-81
references.pmid: 19413972
references.citation: Proc Natl Acad Sci U S A. 2005 May 10;102(19):6873-8
references.pmid: 15863618
references.citation: Biophys J. 2008 Dec 15;95(12):5798-808
references.pmid: 18835889
references.citation: J Physiol. 1992;451:247-78
references.pmid: 1403812
references.citation: Ann Biomed Eng. 2008 Oct;36(10):1624-40
references.pmid: 18642081
references.citation: Nat Cell Biol. 2005 Sep;7(9):861-9
references.pmid: 16100513
references.citation: Nat Struct Biol. 2003 Oct;10(10):831-5
references.pmid: 14502269
references.citation: Proc Natl Acad Sci U S A. 1996 Apr 30;93(9):4459-64
references.pmid: 8633089
references.citation: Biochemistry. 2004 Dec 14;43(49):15404-17
references.pmid: 15581352
references.citation: Eur Biophys J. 2002 Jul;31(4):268-74
references.pmid: 12122473
references.citation: Cell. 2004 Mar 5;116(5):737-49
references.pmid: 15006355
references.citation: Biophys J. 2008 Oct;95(7):3322-9
references.pmid: 18599626
references.citation: Hung Acta Physiol. 1949;2(1-4):120-33
references.pmid: 15397948
references.citation: Nature. 2003 Sep 25;425(6956):423-7
references.pmid: 14508495