title: Antidiabetic and antioxidant effects of extracts from Potentilla discolor Bunge on diabetic rats induced by high fat diet and streptozotocin.
abstract: OBJECTIVE
  Potentilla discolor Bunge, commonly found at the north temperate and boreal zone, has been used for diabetes in China for a long time. Flavonoids and triterpenoids are two major types of compounds in P. discolor. This study was designed primarily to investigate the effects of total flavonoids extract (TFE) and total triterpenoids extract (TTE) of P. discolor Bunge on blood glucose, lipid profiles and antioxidant parameters on diabetic rats induced by high fat diet and streptozotocin.
  
  
  METHODS
  High fat diet-fed and streptozotocin-induced diabetic rats were treated with the TFE and TTE for 15 days, respectively. A range of parameters were tested including fasting blood glucose (FBG), serum insulin (SI), blood lipid profile, malondialdehyde (MDA), superoxide dismutase (SOD), glutathione (GSH), glycosylated serum protein (GSP), and nitric oxide (NO).
  
  
  RESULTS
  Diabetic rats treated with TFE or TTE had decreased concentration of FBG and GSP compared with the control group. Meanwhile, the levels of serum total cholesterol (TC), triglycerides (TG) and low-density lipoprotein cholesterol (LDL-c) in the TFE or TTE treated diabetic rats were lower, and the high-density lipoprotein cholesterol (HDL-c) level was higher than in the control diabetic rats. Furthermore, the extracts treatment decreased the MDA and NO level, while increased SOD and GSH levels in diabetic rats. Histopathologic examination also showed that the extracts have protective effects on β-cells in diabetic rats which are supported by the increase of SI.
  
  
  CONCLUSIONS
  All these experimental results highlighted the hypoglycemic and hypolipidemic properties of the two extracts from Potentilla discolor Bunge on diabetes and its complications, possibly through a strong antioxidant activity and a protective action on β-cells.
authors.affiliation: Department of Chinese Materia Medica Analysis, China Pharmaceutical University, Nanjing 210009, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Jie
authors.initials: J
authors.lastname: Yang
authors.forename: Xiao-qing
authors.initials: XQ
authors.lastname: Chen
authors.forename: Ke
authors.initials: K
authors.lastname: Zan
authors.forename: Xiao-dong
authors.initials: XD
authors.lastname: Wen
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wang
authors.forename: Mao-xiang
authors.initials: MX
authors.lastname: Lai
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001402:B-Lymphocytes / Q000187:drug effects; D001786:Blood Glucose; D001798:Blood Proteins / Q000378:metabolism; D001835:Body Weight / Q000187:drug effects; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000188:drug therapy*; D004041:Dietary Fats / Q000008:administration & dosage; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005419:Flavonoids / Q000494:pharmacology; D006023:Glycoproteins / Q000378:metabolism; D006949:Hyperlipidemias / Q000097:blood / Q000188:drug therapy*; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D000960:Hypolipidemic Agents / Q000494:pharmacology*; D007328:Insulin / Q000097:blood; D008055:Lipids / Q000097:blood; D008297:Male; D008517:Phytotherapy; D031987:Potentilla; D051381:Rats; D017208:Rats, Wistar; D000093422:Glycated Serum Proteins
chemical_list: D000975:Antioxidants; D001786:Blood Glucose; D001798:Blood Proteins; D004041:Dietary Fats; D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D006023:Glycoproteins; D007004:Hypoglycemic Agents; D000960:Hypolipidemic Agents; D007328:Insulin; D008055:Lipids; D000093422:Glycated Serum Proteins