Preliminary crystallographic analysis of the Escherichia coli antitoxin MqsA (YgiT/b3021) in complex with mqsRA promoter DNA.
The Escherichia coli proteins MqsR and MqsA comprise a novel toxin-antitoxin (TA) system. MqsA, the antitoxin, defines a new family of antitoxins because unlike other antitoxins MqsA is structured throughout its entire sequence, binds zinc and coordinates DNA via its C-terminal and not its N-terminal domain. In order to understand how bacterial antitoxins, and MqsA in particular, regulate transcription, the MqsA protein was cocrystallized with a 26-mer duplex DNA corresponding to the palindromic region of the mqsRA promoter. The merohedrally twinned crystal belonged to space group P4(1), with unit-cell parameters a=60.99, b=60.99, c=148.60 A. A complete data set was collected to a resolution of 2.1 A. The solvent content of the crystal was consistent with the presence of two MqsA molecules bound to the duplex DNA in the asymmetric unit.
Brown
Breann L
BL
Department of Molecular Pharmacology, Physiology and Biotechnology, Brown University, Box G-E3, Providence, RI 02912, USA.
Page
Rebecca
R
D018360:Crystallography, X-Ray; D004269:DNA, Bacterial / Q000737:chemistry* / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000737:chemistry*; D004926:Escherichia coli / Q000737:chemistry*; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011485:Protein Binding
D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; C555039:MqsA protein, E coli; C508571:MqsR protein, E coli
Science. 2004 Sep 10;305(5690):1622-5
15308767
J Bacteriol. 1986 Apr;166(1):100-4
3007430
Proc Natl Acad Sci U S A. 1995 Apr 11;92(8):3274-7
7724551
Methods Enzymol. 1997;276:307-26
27754618
Mol Cell. 2005 Aug 19;19(4):497-509
16109374
Mol Cell. 2003 Apr;11(4):875-84
12718874
Protein Sci. 2003 Sep;12(9):1865-71
12930986
J Bacteriol. 2007 Sep;189(17):6089-92
17616596
Science. 2009 Jan 16;323(5912):396-401
19150849
J Bacteriol. 2004 Dec;186(24):8172-80
15576765
J Mol Biol. 1968 Apr 28;33(2):491-7
5700707
Protein Expr Purif. 2007 Jan;51(1):1-10
16904906
Acta Crystallogr D Biol Crystallogr. 2001 Jun;57(Pt 6):854-6
11375506
J Biol Chem. 2009 Oct 16;284(42):28746-53
19690171
J Mol Biol. 2008 Nov 14;383(3):482-93
18793646
Acta Crystallogr D Biol Crystallogr. 2006 Jan;62(Pt 1):72-82
16369096
J Mol Biol. 2003 Sep 26;332(4):809-19
12972253
PLoS Pathog. 2009 Dec;5(12):e1000706
20041169
Nat Struct Mol Biol. 2005 Apr;12(4):327-31
15768033