title: MicroRNA-24 targeting RNA-binding protein DND1 in tongue squamous cell carcinoma.
abstract: Deregulations of microRNA have been frequently observed in tongue squamous cell carcinoma (TSCC), but their roles in tumorigenesis are not entirely clear. Here, we reported the up-regulation of miR-24 in TSCC. MiR-24 up-regulation reduced the expression of RNA-binding protein dead end 1 (DND1). Knockdown of miR-24 led to enhanced expression of DND1. The direct targeting of miR-24 to the DND1 mRNA was predicted bioinformatically and confirmed by luciferase reporter gene assays. Furthermore, the miR-24-mediated change in DND1 expression suppressed the expression of cyclin-dependent kinase inhibitor 1B (CDKN1B), and also led to enhanced proliferation and reduced apoptosis in TSCC cells.
authors.affiliation: Center for Molecular Biology of Oral Diseases, College of Dentistry, University of Illinois at Chicago, Chicago, IL 60612, USA.
authors.forename: Xiqiang
authors.initials: X
authors.lastname: Liu
authors.forename: Anxun
authors.initials: A
authors.lastname: Wang
authors.forename: Caroline E
authors.initials: CE
authors.lastname: Heidbreder
authors.forename: Lu
authors.initials: L
authors.lastname: Jiang
authors.forename: Jinsheng
authors.initials: J
authors.lastname: Yu
authors.forename: Antonia
authors.initials: A
authors.lastname: Kolokythas
authors.forename: Lei
authors.initials: L
authors.lastname: Huang
authors.forename: Yang
authors.initials: Y
authors.lastname: Dai
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Zhou
mesh_terms: D017209:Apoptosis / Q000235:genetics; D001483:Base Sequence; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D049109:Cell Proliferation; D019295:Computational Biology; D050760:Cyclin-Dependent Kinase Inhibitor p27; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000037:antagonists & inhibitors / Q000235:genetics; D035683:MicroRNAs / Q000378:metabolism*; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D017423:Sequence Analysis, RNA; D014062:Tongue Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D015854:Up-Regulation
chemical_list: C436291:CDKN1B protein, human; C528453:Dnd1 protein, human; D047908:Intracellular Signaling Peptides and Proteins; C543845:MIRN24 microRNA, human; D035683:MicroRNAs; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D050760:Cyclin-Dependent Kinase Inhibitor p27
references.citation: Cell. 2007 Dec 28;131(7):1273-86
references.pmid: 18155131
references.citation: Genome Res. 2009 Jan;19(1):92-105
references.pmid: 18955434
references.citation: Mol Cancer. 2007 Jan 14;6:5
references.pmid: 17222355
references.citation: CA Cancer J Clin. 2009 Jul-Aug;59(4):225-49
references.pmid: 19474385
references.citation: Cancer Res. 2008 Apr 1;68(7):2094-105
references.pmid: 18381414
references.citation: Nucleic Acids Res. 2008 Jan;36(Database issue):D154-8
references.pmid: 17991681
references.citation: Cell. 2006 Jun 16;125(6):1111-24
references.pmid: 16777601
references.citation: PLoS One. 2008 Jul 02;3(7):e2557
references.pmid: 18596939
references.citation: Carcinogenesis. 1991 Sep;12(9):1627-31
references.pmid: 1654226
references.citation: PLoS One. 2008 Mar 26;3(3):e1864
references.pmid: 18365017
references.citation: CA Cancer J Clin. 2005 Jan-Feb;55(1):10-30
references.pmid: 15661684
references.citation: Cancer Genomics Proteomics. 2009 May-Jun;6(3):131-9
references.pmid: 19487542
references.citation: Nat Rev Genet. 2009 Oct;10(10):704-14
references.pmid: 19763153
references.citation: Biochem Biophys Res Commun. 2007 Jun 22;358(1):12-7
references.pmid: 17475218
references.citation: Nat Genet. 2005 May;37(5):495-500
references.pmid: 15806104
references.citation: Nature. 2005 May 19;435(7040):360-4
references.pmid: 15902260
references.citation: Proc Natl Acad Sci U S A. 2006 Feb 14;103(7):2257-61
references.pmid: 16461460
references.citation: Cell Mol Life Sci. 2007 Jun;64(11):1317-22
references.pmid: 17464447
references.citation: Oral Oncol. 2010 Mar;46(3):204-8
references.pmid: 20138800
references.citation: Methods. 2001 Dec;25(4):402-8
references.pmid: 11846609
references.citation: Cancer Lett. 2009 Dec 28;286(2):217-22
references.pmid: 19540661
references.citation: Clin Cancer Res. 2008 May 1;14(9):2588-92
references.pmid: 18451220
references.citation: Oncol Res. 2010;18(8):377-85
references.pmid: 20441052