Development of a simultaneous partial nitrification and anaerobic ammonia oxidation process in a single reactor.
Up-flow oxygen-controlled biofilm reactors equipped with a non-woven fabric support were used as a single reactor system for autotrophic nitrogen removal based on a combined partial nitrification and anaerobic ammonium oxidation (anammox) reaction. The up-flow biofilm reactors were initiated as either a partial nitrifying reactor or an anammox reactor, respectively, and simultaneous partial nitrification and anammox was established by careful control of the aeration rate. The combined partial nitrification and anammox reaction was successfully developed in both biofilm reactors without additional biomass inoculation. The reactor initiated as the anammox reactor gave a slightly higher and more stable mean nitrogen removal rate of 0.35 (±0.19) kg-N m(-3) d(-1) than the reactor initiated as the partial nitrifying reactor (0.23 (±0.16) kg-N m(-3) d(-1)). FISH analysis revealed that the biofilm in the reactor started as the anammox reactor were composed of anammox bacteria located in inner anoxic layers that were surrounded by surface aerobic AOB layers, whereas AOB and anammox bacteria were mixed without a distinguishable niche in the biofilm in the reactor started as the partial nitrifying reactor. However, it was difficult to efficiently maintain the stable partial nitrification owing to inefficient aeration in the reactor, which is a key to development of the combined partial nitrification and anammox reaction in a single biofilm reactor.
Cho
Sunja
S
Department of Civil and Environmental Engineering, Pusan National University, Busan 609-735, Korea.
Fujii
Naoki
N
Lee
Taeho
T
Okabe
Satoshi
S
D000641:Ammonia / Q000378:metabolism*; D000693:Anaerobiosis; D052818:Autotrophic Processes / Q000502:physiology; D001419:Bacteria / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D018441:Biofilms / Q000254:growth & development; D019149:Bioreactors / Q000382:microbiology*; D058645:Denaturing Gradient Gel Electrophoresis; D052918:Environmental Restoration and Remediation / Q000295:instrumentation* / Q000379:methods*; D016650:Fluorescein-5-isothiocyanate / Q000378:metabolism; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D058465:Nitrification / Q000502:physiology*; D009584:Nitrogen / Q000302:isolation & purification; D010084:Oxidation-Reduction; D010802:Phylogeny; D025341:Principal Component Analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics
D012336:RNA, Ribosomal, 16S; D000641:Ammonia; D016650:Fluorescein-5-isothiocyanate; D009584:Nitrogen