Enzymatic single-step preparation of multifunctional proteins.
A small glutamine donor substrate was designed for single-step and site-specific protein multifunctionalization catalyzed by microbial transglutaminase.
Abe
Hiroki
H
Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Motooka, Fukuoka 819-0395, Japan.
Goto
Masahiro
M
Kamiya
Noriho
N
D001710:Biotin / Q000737:chemistry; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D005973:Glutamine / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D013302:Streptomyces / Q000201:enzymology*; D013379:Substrate Specificity; D011503:Transglutaminases / Q000378:metabolism*
D005456:Fluorescent Dyes; D011506:Proteins; D011994:Recombinant Proteins; D005973:Glutamine; D001710:Biotin; D011503:Transglutaminases; D016650:Fluorescein-5-isothiocyanate