Acquisition of iron by alkaliphilic bacillus species.
The biochemical and molecular mechanisms used by alkaliphilic bacteria to acquire iron are unknown. We demonstrate that alkaliphilic (pH > 9) Bacillus species are sensitive to artificial iron (Fe³+) chelators and produce iron-chelating molecules. These alkaliphilic siderophores contain catechol and hydroxamate moieties, and their synthesis is stimulated by manganese(II) salts and suppressed by FeCl₃ addition. Purification and mass spectrometric characterization of the siderophore produced by Caldalkalibacillus thermarum failed to identify any matches to previously observed fragmentation spectra of known siderophores, suggesting a novel structure.
McMillan
Duncan G G
DG
Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand.
Velasquez
Imelda
I
Nunn
Brook L
BL
Goodlett
David R
DR
Hunter
Keith A
KA
Lamont
Iain
I
Sander
Sylvia G
SG
Cook
Gregory M
GM
D001406:Bacillaceae / Q000378:metabolism*; D002396:Catechols / Q000032:analysis; D002712:Chlorides / Q000378:metabolism; D005290:Ferric Compounds / Q000378:metabolism; D006877:Hydroxamic Acids / Q000032:analysis; D007501:Iron / Q000378:metabolism*; D007502:Iron Chelating Agents / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D008345:Manganese / Q000378:metabolism; D013058:Mass Spectrometry; D017262:Siderophores / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism
D002396:Catechols; D002712:Chlorides; D005290:Ferric Compounds; D006877:Hydroxamic Acids; D007502:Iron Chelating Agents; D017262:Siderophores; D008345:Manganese; D007501:Iron; C034221:catechol; C024555:ferric chloride
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