title: Structure-based investigation on the binding interaction of hydroxylated polybrominated diphenyl ethers with thyroxine transport proteins.
abstract: Polybrominated diphenyl ethers (PBDEs) have been shown to alter thyroid hormone level in experimental animals. One of the possible mechanisms for hormone disruption is the competitive binding of hydroxylated PBDEs (OH-PBDEs) with hormone transport proteins. In this study, binding interaction of 14 diversely structured OH-PBDEs with two thyroxine transport proteins was investigated by fluorescence displacement assay, circular dichroism, and molecular docking. Binding affinity of the 14 OH-PBDEs with transthyretin (TTR) and thyroxine-binding globulin (TBG) was measured by competitive fluorescence displacement assay. The binding constant was found to fall in the range of 1.4 x 10⁷ M and 6.9 x 10⁸ M⁻¹ for TTR, and between 6.5 x 10⁶ M⁻¹ and 2.2 x 10⁸ M⁻¹ for TBG. Binding affinity increased significantly with bromination number from 1 to 4, whereas 5- and 6-brominated diphenyl ethers did not show any further increase. Protein secondary structural change of TTR and TBG upon binding with 5-OH-BDE-047 was investigated by circular dichroism. The spectral change displayed a pattern similar to the one with thyroxine, suggesting that the environmental chemical binds to the two proteins at the same sites as the hormone. In molecular docking analysis, a ligand-binding channel in TTR was revealed for OH-PBDEs binding, which appeared to be mostly hydrophobic inside but guarded by positively charged residue Lys15 at the entrance. Binding affinity of the 14 OH-PBDEs with TTR could be rationalized reasonably well by their pocket binding mode and hydrophobic characteristics. Based on the binding constant obtained in this work, possibility of in vitro competitive displacement of thyroid hormones from the transport proteins by OH-PBDEs was evaluated.
authors.affiliation: State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-environmental Sciences, Chinese Academy of Sciences, P.O. Box 2871, Beijing 100085, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Cao
authors.forename: Yuan
authors.initials: Y
authors.lastname: Lin
authors.forename: Liang-Hong
authors.initials: LH
authors.lastname: Guo
authors.forename: Ai-Qian
authors.initials: AQ
authors.lastname: Zhang
authors.forename: Yin
authors.initials: Y
authors.lastname: Wei
authors.forename: Yu
authors.initials: Y
authors.lastname: Yang
mesh_terms: D001667:Binding, Competitive / Q000502:physiology; D018360:Crystallography, X-Ray; D055768:Halogenated Diphenyl Ethers / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D006900:Hydroxylation / Q000502:physiology; D008958:Models, Molecular; D011485:Protein Binding / Q000187:drug effects / Q000502:physiology; D013050:Spectrometry, Fluorescence; D013329:Structure-Activity Relationship; D013963:Thyroid Hormones / Q000378:metabolism; D013975:Thyroxine-Binding Proteins / Q000378:metabolism*
chemical_list: D055768:Halogenated Diphenyl Ethers; D013963:Thyroid Hormones; D013975:Thyroxine-Binding Proteins