title: Regeneration and transdetermination: the role of wingless and its regulation.
abstract: Imaginal discs of Drosophila have the remarkable ability to regenerate. After fragmentation wound healing occurs, ectopic wg is induced and a blastema is formed. In some, but not all fragments, the blastema will replace missing structures and a few cells can become more plastic and transdetermine to structures of other discs. A series of systematic cuts through the first leg disc revealed that a cut must transect the dorsal-proximal disc area and that the fragment must also include wg-competent cells. Fragments that fail to both transdetermine and regenerate missing structures will do both when provided with exogenous Wg, demonstrating the necessity of Wg in regenerative processes. In intact leg discs ubiquitously expressed low levels of Wg also leads to blastema formation, regeneration and transdetermination. Two days after exogenous wg induction the endogenous gene is activated, leading to elevated levels of Wg in the dorsal aspect of the leg disc. We identified a wg enhancer that regulates ectopic wg expression. Deletion of this enhancer increases transdetermination, but lowers the amount of ectopic Wg. We speculate that this lessens repression of dpp dorsally, and thus creates a permissive condition under which the balance of ectopic Wg and Dpp is favorable for transdetermination.
authors.affiliation: Department of Biology, Box 351800, University of Washington, Seattle, WA 98105, USA. margrit@u.washington.edu
authors.forename: Margrit
authors.initials: M
authors.lastname: Schubiger
authors.forename: Anne
authors.initials: A
authors.lastname: Sustar
authors.forename: Gerold
authors.initials: G
authors.lastname: Schubiger
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D029721:Drosophila Proteins / Q000235:genetics* / Q000502:physiology*; D004331:Drosophila melanogaster / Q000235:genetics* / Q000254:growth & development / Q000502:physiology*; D004742:Enhancer Elements, Genetic; D005121:Extremities / Q000254:growth & development / Q000502:physiology; D017344:Genes, Insect; D012038:Regeneration / Q000235:genetics* / Q000502:physiology*; D015533:Transcriptional Activation; D051155:Wnt1 Protein / Q000235:genetics* / Q000502:physiology*
chemical_list: D017931:DNA Primers; D029721:Drosophila Proteins; D051155:Wnt1 Protein; C064196:dpp protein, Drosophila; C074622:wg protein, Drosophila
references.citation: Development. 1995 Oct;121(10):3359-69
references.pmid: 7588069
references.citation: Wilhelm Roux Arch Entwickl Mech Org. 1968 Mar;160(1):41-75
references.pmid: 28304493
references.citation: Dev Cell. 2009 Mar;16(3):358-73
references.pmid: 19289082
references.citation: EMBO J. 1993 Dec 15;12(13):5293-302
references.pmid: 8262072
references.citation: Wilhelm Roux Arch Entwickl Mech Org. 1968 Sep;161(3):249-280
references.pmid: 28304471
references.citation: Nature. 2005 Nov 10;438(7065):234-7
references.pmid: 16281037
references.citation: Proc Natl Acad Sci U S A. 2008 Jul 15;105(28):9715-20
references.pmid: 18621688
references.citation: Dev Dyn. 2003 Feb;226(2):202-10
references.pmid: 12557199
references.citation: Development. 1998 Aug;125(15):2771-80
references.pmid: 9655800
references.citation: Nature. 2009 Jul 2;460(7251):60-5
references.pmid: 19571878
references.citation: J Biol. 2004;3(3):11
references.pmid: 15186480
references.citation: Wilhelm Roux Arch Entwickl Mech Org. 1933 Sep;130(3-4):747-770
references.pmid: 28354371
references.citation: Science. 1996 Sep 6;273(5280):1373-7
references.pmid: 8703069
references.citation: Development. 2005 Aug;132(16):3753-65
references.pmid: 16077094
references.citation: Dev Biol. 1976 Feb;48(2):461-5
references.pmid: 815114
references.citation: Science. 2008 Jan 18;319(5861):327-30
references.pmid: 18063755
references.citation: Development. 2009 Apr;136(7):1169-77
references.pmid: 19244279
references.citation: Dev Biol. 2008 Jul 1;319(1):68-77
references.pmid: 18485344
references.citation: Cold Spring Harb Symp Quant Biol. 2008;73:583-92
references.pmid: 19028989
references.citation: Dev Cell. 2009 Jun;16(6):797-809
references.pmid: 19531351
references.citation: Nature. 1994 Dec 1;372(6505):461-4
references.pmid: 7984239
references.citation: PLoS Biol. 2010 Jun 08;8(6):e1000390
references.pmid: 20543996
references.citation: Science. 2005 Dec 23;310(5756):1919-23
references.pmid: 16373567
references.citation: Dev Biol. 1970 Jul;22(3):389-411
references.pmid: 5423309
references.citation: Dev Biol. 1971 Oct;26(2):277-95
references.pmid: 5003476
references.citation: Development. 2007 Feb;134(3):479-89
references.pmid: 17185322
references.citation: Wilhelm Roux Arch Entwickl Mech Org. 1970 Mar;166(1):1-44
references.pmid: 28304536
references.citation: Bioessays. 1995 Apr;17(4):299-309
references.pmid: 7741723
references.citation: Development. 1995 May;121(5):1263-72
references.pmid: 7789260
references.citation: Mech Dev. 2000 Jul;95(1-2):89-99
references.pmid: 10906453
references.citation: Dev Biol. 1993 May;157(1):240-50
references.pmid: 8482414
references.citation: Dev Biol. 1988 May;127(1):197-208
references.pmid: 2452103
references.citation: Proc Natl Acad Sci U S A. 1975 Nov;72(11):4674-8
references.pmid: 812100
references.citation: Development. 1996 Nov;122(11):3519-29
references.pmid: 8951067
references.citation: Nature. 1994 Mar 17;368(6468):208-14
references.pmid: 8145818
references.citation: Wilhelm Roux Arch Entwickl Mech Org. 1968 Mar;160(1):9-40
references.pmid: 28304492
references.citation: Cell. 2005 Feb 11;120(3):383-93
references.pmid: 15707896
references.citation: Dev Biol. 1983 May;97(1):212-21
references.pmid: 6404675
references.citation: Experientia. 1973 May 15;29(5):631-2
references.pmid: 4199754
references.citation: J Embryol Exp Morphol. 1974 Jun;31(3):557-72
references.pmid: 4448938
references.citation: Genes Dev. 2006 Dec 1;20(23):3232-7
references.pmid: 17114576
references.citation: J Exp Zool. 1975 Jul;193(1):49-77
references.pmid: 806653
references.citation: PLoS One. 2010 Jan 07;5(1):e8510
references.pmid: 20062803
references.citation: Wilhelm Roux Arch Entwickl Mech Org. 1935 Jan;133(1-2):156-192
references.pmid: 28354978
references.citation: Dev Biol. 1981 Oct 15;87(1):64-75
references.pmid: 7286422
references.citation: J Embryol Exp Morphol. 1966 Feb;15(1):77-111
references.pmid: 5915174
references.citation: J Exp Zool. 1981 Apr;216(1):45-61
references.pmid: 6793689
references.citation: Science. 2008 Jan 18;319(5861):323-7
references.pmid: 18063757
references.citation: Development. 2010 Apr;137(7):1169-79
references.pmid: 20215351
references.citation: Dev Biol. 2005 Apr 1;280(1):73-86
references.pmid: 15766749
references.citation: Proc Natl Acad Sci U S A. 2009 Mar 17;106(11):4290-5
references.pmid: 19237582
references.citation: Development. 1999 Apr;126(8):1591-9
references.pmid: 10079222
references.citation: Cell. 1993 Feb 26;72(4):527-40
references.pmid: 8440019
references.citation: Wilehm Roux Arch Dev Biol. 1977 Jun;183(2):85-100
references.pmid: 28304897
references.citation: Int J Dev Biol. 2005;49(4):391-9
references.pmid: 15968584