The potential benefits of a garlic and hydrochlorothiazide combination as antihypertensive and cardioprotective in rats.
The purpose of the present study was to investigate the protective effects of combined therapy of garlic homogenate and hydrochlorothiazide (HCTZ) in animals with hypertension and myocardial damage. Three weeks of high fructose (10% w/v) in fluid to albino rats resulted in hypertension. They were subsequently administered garlic (125, 250 and 500 mg/kg, 3 weeks in separate groups) and HCTZ (10 mg/kg, 6th week) once daily in their respective groups. At the end of 6 weeks, myocardial damage was induced by subcutaneous administration of isoproterenol (175 mg/kg) for 2 consecutive days. The results showed that garlic and HCTZ increase the lactate dehydrogenase, creatinine phosphokinase, superoxide dismutase and catalase activities in heart homogenate when used concurrently or separately. Further, restoration of normal values in fluid/food intake, body weight, systolic blood pressure, cholesterol, triglycerides, glucose and histopathological scores were observed in all treated groups. Furthermore, histological disturbances and hypertension were significantly ameliorated in treated animals. Moreover, moderate dose of garlic was more effective than low dose, while a high dose of garlic was least effective in correcting electrocardiographic changes. Thus it is concluded that garlic in moderate dose (250 mg/kg) with added HCTZ possesses synergistic cardioprotective and antihypertensive properties against fructose- and isoproterenol-induced toxicities.
Asdaq
Syed Mohammed Basheeruddin
SM
Department of Pharmacology, Krupanidhi College of Pharmacy, Varthur Hobli, Chikkabellandur Village, Carmalaram Post, Bangalore 560 035, India. basheer_1@rediffmail.com
Inamdar
Mohammed Naseeruddin
MN
D000818:Animals; D000959:Antihypertensive Agents / Q000737:chemistry / Q000494:pharmacology*; D001794:Blood Pressure / Q000187:drug effects; D004562:Electrocardiography; D005260:Female; D005737:Garlic / Q000737:chemistry*; D006321:Heart / Q000187:drug effects*; D006852:Hydrochlorothiazide / Q000494:pharmacology*; D007545:Isoproterenol / Q000633:toxicity; D009206:Myocardium / Q000473:pathology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
D000959:Antihypertensive Agents; D010936:Plant Extracts; D006852:Hydrochlorothiazide; D007545:Isoproterenol
J Cell Physiol. 1994 Jan;158(1):133-9
8263020
Basic Res Cardiol. 1995 Nov-Dec;90(6):467-74
8773192
J Am Coll Cardiol. 2004 Nov 16;44(10):1996-2002
15542282
Indian J Physiol Pharmacol. 2009 Apr-Jun;53(2):127-36
20112816
Arch Biochem Biophys. 1988 Sep;265(2):362-72
3421711
J Pharmacol Exp Ther. 1998 Jul;286(1):429-38
9655888
Clin Chem. 1983 Mar;29(3):538-42
6825269
Lab Invest. 1981 May;44(5):426-33
7230729
J Ethnopharmacol. 2004 Nov;95(1):57-61
15374607
Nature. 1993 Apr 29;362(6423):801-9
8479518
Drugs. 1994 May;47(5):711-33
7520854
Int J Cardiol. 2007 Feb 14;115(3):326-33
16828181
BMC Pharmacol. 2002 Aug 16;2:16
12182765
J Med Food. 2006 Summer;9(2):205-13
16822206
Carcinogenesis. 1987 Aug;8(8):1155-7
3608098
Eur Heart J. 1993 May;14(5):677-81
7685286
Natl Med J India. 2000 Nov-Dec;13(6):304-10
11209486
Chem Biol Interact. 2009 Oct 30;181(3):472-9
19660444
N Engl J Med. 1997 Apr 17;336(16):1117-24
9099655
J Clin Invest. 1998 Oct 1;102(7):1377-84
9769330
J Clin Invest. 1984 Nov;74(5):1792-802
6501571
N Engl J Med. 2001 Jan 4;344(1):3-10
11136953
N Engl J Med. 1989 May 4;320(18):1177-82
2624617
Fitoterapia. 2001 May;72(4):402-5
11395263
J Trop Pediatr. 2001 Jun;47(3):181-5
11419685
J Ethnopharmacol. 2001 Aug;76(3):215-21
11448541
Am J Cardiol. 1990 Mar 6;65(10):4E-9E; discussion 22E-23E
2178377
J Mol Cell Cardiol. 1985 Apr;17(4):291-306
3894676
J Am Soc Nephrol. 1990 Jul;1(1):43-52
2104250
Clin Chem. 1982 Oct;28(10):2077-80
6812986