title: Characterization of Bacillus phage-K2 isolated from chungkookjang, a fermented soybean foodstuff.
abstract: An investigation of a virulent Bacillus phage-K2 (named Bp-K2) isolated from chungkookjang (a fermented soybean foodstuff) was made. Bp-K2 differed in infectivity against a number of Bacillus subtilis strains including starter strains of chungkookjang and natto, being more infectious to Bacillus strains isolated from the chungkookjang, but much less active against a natto strain. Bp-K2 is a small DNA phage whose genome size is about 21 kb. Bp-K2 is a tailed bacteriophage with an isometric icosahedral head (50 nm long on the lateral side, 80 nm wide), a long contractile sheath (85-90 nm × 28 nm), a thin tail fiber (80-85 nm long, 10 nm wide), and a basal plate (29 nm long, 47 nm wide) with a number of spikes, but no collar. The details of the structures of Bp-K2 differ from natto phage ϕBN100 as well as other known Bacillus phages such as SPO1-like or ϕ 29-like viruses. These data suggest that Bp-K2 would be a new member of the Myoviridae family of Bacillus bacteriophages.
authors.affiliation: Department of Microbiology, Chungbuk National University, Cheongju, Chungbuk, 361-763, Korea.
authors.forename: Eun Ju
authors.initials: EJ
authors.lastname: Kim
authors.forename: Jeong Won
authors.initials: JW
authors.lastname: Hong
authors.forename: Na-Rae
authors.initials: NR
authors.lastname: Yun
authors.forename: Young Nam
authors.initials: YN
authors.lastname: Lee
mesh_terms: D017103:Bacillus Phages / Q000145:classification / Q000302:isolation & purification* / Q000648:ultrastructure; D001412:Bacillus subtilis / Q000821:virology; D005285:Fermentation; D005516:Food Microbiology; D046529:Microscopy, Electron, Transmission; D017900:Myoviridae / Q000145:classification / Q000302:isolation & purification* / Q000648:ultrastructure; D013025:Glycine max / Q000821:virology*
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