Characterization of a two-component regulatory system that regulates succinate-mediated catabolite repression in Sinorhizobium meliloti.
When they are available, Sinorhizobium meliloti utilizes C(4)-dicarboxylic acids as preferred carbon sources for growth while suppressing the utilization of some secondary carbon sources such as α- and β-galactosides. The phenomenon of using succinate as the sole carbon source in the presence of secondary carbon sources is termed succinate-mediated catabolite repression (SMCR). Genetic screening identified the gene sma0113 as needed for strong SMCR when S. meliloti was grown in succinate plus lactose, maltose, or raffinose. sma0113 and the gene immediately downstream, sma0114, encode the proteins Sma0113, an HWE histidine kinase with five PAS domains, and Sma0114, a CheY-like response regulator lacking a DNA-binding domain. sma0113 in-frame deletion mutants show a relief of catabolite repression compared to the wild type. sma0114 in-frame deletion mutants overproduce polyhydroxybutyrate (PHB), and this overproduction requires sma0113. Sma0113 may use its five PAS domains for redox level or energy state monitoring and use that information to regulate catabolite repression and related responses.
Garcia
Preston P
PP
University of Connecticut, Department of Molecular and Cell Biology, 91 N. Eagleville Rd., U-3125, Storrs, CT 06269-3125, USA.
Bringhurst
Ryan M
RM
Arango Pinedo
Catalina
C
Gage
Daniel J
DJ
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002244:Carbon / Q000378:metabolism*; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D007785:Lactose; D000455:Medicago sativa / Q000382:microbiology / Q000502:physiology; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009154:Mutation; D010641:Phenotype; D055170:Plant Root Nodulation / Q000502:physiology; D011487:Protein Conformation; D016962:Sinorhizobium meliloti / Q000235:genetics / Q000378:metabolism*; D013386:Succinates / Q000378:metabolism*; D013559:Symbiosis
D001426:Bacterial Proteins; D013386:Succinates; D002244:Carbon; D007785:Lactose
J Bacteriol. 2007 May;189(9):3445-51
17337584
J Biol Chem. 2005 May 27;280(21):20596-603
15781452
Nature. 1997 Aug 7;388(6642):539-47
9252185
J Bacteriol. 2006 Nov;188(21):7354-63
17050923
Annu Rev Genet. 2007;41:121-45
18076326
Plant Cell. 1996 Oct;8(10):1885-98
8914326
Plant Cell. 1995 Jul;7(7):869-885
12242391
New Phytol. 1992 Oct;122(2):211-237
33873995
J Mol Biol. 1999 Jun 25;289(5):1423-33
10373376
Science. 1992 May 15;256(5059):998-1000
10744524
Microbiology (Reading). 2004 Apr;150(Pt 4):877-883
15073297
J Bacteriol. 2002 Oct;184(19):5385-92
12218025
Microbiol Mol Biol Rev. 1999 Jun;63(2):479-506
10357859
Proc Natl Acad Sci U S A. 1998 Sep 29;95(20):11951-6
9751771
J Biol Chem. 1991 May 5;266(13):8348-54
1902474
Methods Enzymol. 1991;204:398-418
1658566
Annu Rev Microbiol. 1999;53:103-28
10547687
J Bacteriol. 1982 Jan;149(1):114-22
6274841
Mol Microbiol. 1994 Jan;11(1):165-73
8145640
Nucleic Acids Res. 1987 Oct 12;15(19):7921-34
3671068
Mol Microbiol. 1989 Jun;3(6):813-23
2546011
Nature. 1989 Feb 23;337(6209):745-9
2645526
Curr Opin Microbiol. 2009 Apr;12(2):152-60
19246239
Gene. 1983 Dec;26(2-3):197-203
6323260
J Biol Chem. 2002 Oct 18;277(42):39809-14
12167640
Biochemistry. 1993 Nov 9;32(44):11741-53
8218244
J Bacteriol. 2004 Jan;186(2):445-53
14702314
J Bacteriol. 2006 Nov;188(21):7328-30
17050920
J Mol Microbiol Biotechnol. 2009;17(4):201-10
19797916
Gene. 1993 May 15;127(1):15-21
8486283
Gene. 1989 Apr 15;77(1):51-9
2744487
Environ Microbiol. 2004 Jun;6(6):552-67
15142243
Environ Microbiol. 2008 May;10(5):1320-34
18279347
Mol Genet Genomics. 2004 Aug;272(1):1-17
15221452
Arch Microbiol. 1999 Jan;171(2):73-80
9914303
FEMS Microbiol Rev. 2004 Oct;28(4):489-501
15374663
J Biol Chem. 1989 Dec 25;264(36):21770-8
2689446
Annu Rev Biochem. 1996;65:503-35
8811188
J Bacteriol. 1995 Jun;177(11):3058-66
7768801
Biotechniques. 2000 Aug;29(2):240-2, 244-5
10948423
J Bacteriol. 2005 Feb;187(3):1161-72
15659691
Gene. 1989 Dec 21;85(1):135-44
2695394
Annu Rev Phytopathol. 1995;33:345-68
18999965
J Bacteriol. 1998 Nov;180(21):5739-48
9791127
J Bacteriol. 2009 Jan;191(1):298-309
18931135
J Biol Chem. 2005 Jan 14;280(2):1448-56
15522865
Genetics. 1988 Nov;120(3):621-3
2852134
J Bacteriol. 2009 Oct;191(19):5890-900
19633078
Annu Rev Microbiol. 2009;63:133-54
19575571
Cell. 2008 May 2;133(3):452-61
18455986
FEMS Microbiol Ecol. 2008 Sep;65(3):391-9
18631180
J Bacteriol. 1988 Sep;170(9):4257-65
2842308
Proc Natl Acad Sci U S A. 2006 Nov 21;103(47):17933-8
17101990
J Bacteriol. 2004 Feb;186(3):595-600
14729683
Structure. 2009 Oct 14;17(10):1333-44
19836334
Structure. 2009 Oct 14;17(10):1282-94
19836329
Nucleic Acids Res. 2007 Jan;35(Database issue):D386-90
17135192
J Bacteriol. 1996 Dec;178(24):7159-66
8955397
Annu Rev Biochem. 2000;69:183-215
10966457
J Bacteriol. 1978 Dec;136(3):1197-200
214420
Proc Natl Acad Sci U S A. 2000 May 23;97(11):5830-5
10811894
Proc Natl Acad Sci U S A. 1998 May 26;95(11):5857-64
9600884
J Bacteriol. 2008 Apr;190(8):2947-56
18281401
Annu Rev Cell Biol. 1991;7:191-226
1809347
DNA Res. 2004 Feb 29;11(1):57-65
15141946
J Bacteriol. 1996 Mar;178(6):1646-54
8626293