title: Developmental expression of sorting nexin 3 in the mouse central nervous system.
abstract: We previously reported that sorting nexin 3 (SNX3), a protein belonging to the sorting nexin family, regulates neurite outgrowth in mouse N1E-115 neuroblastoma cells. The snx3 gene is disrupted in patients with microcephaly, microphthalmia, ectrodactyly, and prognathism (MMEP) and mental retardation, demonstrating that SNX3 plays an important role in the genesis of these organs during development. The present study was designed to determine the expression pattern of snx3 mRNA, particularly in the mouse central nervous system (CNS), from the embryonic stage to adulthood. Whole mount in situ hybridization of embryonic day (E) 9.5 and 10.5 mouse embryos revealed strong positive signals for snx3 mRNA in the forebrain, pharyngeal arches, eyes, and limb buds. In situ hybridization analyses of embryonic and neonatal brain sections revealed that snx3 mRNA is mainly expressed in the cerebral cortex, hippocampus, piriform cortex, cerebellum, and spinal cord. In adulthood, the expression of snx3 mRNA is observed in the cerebral cortex, hippocampus, piriform cortex, and cerebellar neurons. Thus, snx3 mRNA is expressed during neural development and in adult neural tissues, suggesting that SNX3 may play an important role in the development and function of the CNS.
authors.affiliation: Department of Pharmacology, National Research Institute for Child Health and Development, Setagaya, Tokyo 157-8535, Japan.
authors.forename: Reiko
authors.initials: R
authors.lastname: Mizutani
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Nakamura
authors.forename: Shigetoshi
authors.initials: S
authors.lastname: Yokoyama
authors.forename: Atsushi
authors.initials: A
authors.lastname: Sanbe
authors.forename: Shinji
authors.initials: S
authors.lastname: Kusakawa
authors.forename: Yuki
authors.initials: Y
authors.lastname: Miyamoto
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Torii
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Asahara
authors.forename: Haruo
authors.initials: H
authors.lastname: Okado
authors.forename: Junji
authors.initials: J
authors.lastname: Yamauchi
authors.forename: Akito
authors.initials: A
authors.lastname: Tanoue
mesh_terms: D000818:Animals; D002460:Cell Line; D002490:Central Nervous System / Q000196:embryology* / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D051379:Mice; D058305:Sorting Nexins / Q000235:genetics* / Q000378:metabolism*
chemical_list: C533230:Snx3 protein, mouse; D058305:Sorting Nexins