A quantitative proteomic analysis of biofilm adaptation by the periodontal pathogen Tannerella forsythia.
Tannerella forsythia is a Gram-negative anaerobe that is one of the most prominent inhabitants of the sub-gingival plaque biofilm, which is crucial for causing periodontitis. We have used iTRAQ proteomics to identify and quantify alterations in global protein expression of T. forsythia during growth in a biofilm. This is the first proteomic study concentrating on biofilm growth in this key periodontal pathogen, and this study has identified several changes in protein expression. Moreover, we introduce a rigorous statistical method utilising peptide-level intensities of iTRAQ reporters to determine which proteins are significantly regulated. In total, 348 proteins were identified and quantified with the expression of 44 proteins being significantly altered between biofilm and planktonic cells. We identified proteins from all cell compartments, and highlighted a marked upregulation in the relative abundances of predicted outer membrane proteins in biofilm cells. These included putative transport systems and the T. forsythia S-layer proteins. These data and our finding that the butyrate production pathway is markedly downregulated in biofilms indicate possible alterations in host interaction capability. We also identified upregulation of putative oxidative stress response proteins, and showed that biofilm cells are 10 to 20 fold more resistant to oxidative stress. This may represent an important adaptation of this organism to prolonged persistence and immune evasion in the oral cavity.
Pham
Trong Khoa
TK
Biological and Environmental Systems Group, ChELSI Institute, Department of Chemical and Process Engineering, The University of Sheffield, Sheffield, UK.
Roy
Sumita
S
Noirel
Josselin
J
Douglas
Ian
I
Wright
Phillip C
PC
Stafford
Graham P
GP
D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins / Q000032:analysis* / Q000145:classification / Q000378:metabolism; D041963:Bacteroidetes / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D018441:Biofilms / Q000254:growth & development*; D002087:Butyrates; D057225:Data Mining; D030562:Databases, Protein; D007553:Isotope Labeling; D053858:Metabolic Networks and Pathways; D019158:N-Acetylneuraminic Acid; D020543:Proteome / Q000032:analysis / Q000378:metabolism; D040901:Proteomics / Q000379:methods*
D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D002087:Butyrates; D020543:Proteome; D019158:N-Acetylneuraminic Acid