title: Crystallization and preliminary X-ray analysis of a glucansucrase from the dental caries pathogen Streptococcus mutans.
abstract: Glucansucrases encoded by Streptococcus mutans play essential roles in the synthesis of sticky dental plaques. Based on amino-acid sequence similarity, glucansucrases are classified as members of glycoside hydrolase family 70 (GH 70). Data on the crystal structure of GH 70 glucansucrases have yet to be reported. Here, the GH 70 glucansucrase GTF-SI from S. mutans was overexpressed in Escherichia coli strain BL21 (DE3), purified to homogeneity and crystallized using the hanging-drop vapour-diffusion method. Orthorhombic GTF-SI crystals belonging to space group P2(1)2(1)2 were obtained. A diffraction data set was collected to 2.1 A resolution.
authors.affiliation: Department of Food and Nutritional Sciences, Graduate School of Nutritional and Environmental Sciences, University of Shizuoka, Yada 52-1, Suruga-ku, Shizuoka 422-8526, Japan.
authors.forename: Keisuke
authors.initials: K
authors.lastname: Ito
authors.forename: Sohei
authors.initials: S
authors.lastname: Ito
authors.forename: Tatsuro
authors.initials: T
authors.lastname: Shimamura
authors.forename: Yasuaki
authors.initials: Y
authors.lastname: Kawarasaki
authors.forename: Keiko
authors.initials: K
authors.lastname: Abe
authors.forename: Takumi
authors.initials: T
authors.lastname: Misaka
authors.forename: Takuya
authors.initials: T
authors.lastname: Kobayashi
authors.forename: So
authors.initials: S
authors.lastname: Iwata
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D016695:Glycosyltransferases / Q000737:chemistry*; D013295:Streptococcus mutans / Q000201:enzymology*
chemical_list: D016695:Glycosyltransferases; C033837:alternansucrase
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