Parametric analysis of thermal preference following sleep deprivation in the rat.
A thermal preference task was used to assess the effects of sleep deprivation on nociceptive behavior using hot and cool stimuli. The thermal preference apparatus allowed male rats to move freely from a hot thermal plate (44.7°C) to an adjacent plate at neutral (33.5°C) or cold temperatures (1.3-11°C). Investigators recorded occupancy on the colder side, frequency of movements between the 2 compartments, and first escape latency from the cold side. Parametric analysis of thermal preference indicated that behavioral allocation was related to temperature ranges previously associated with activation of thermal nociceptors. A 50% occupancy rate was determined from a stimulus-response function identifying 1.3°C vs. 44.7°C as optimal temperatures. This temperature combination was then used to test the effects of sleep deprivation for 48h using the pedestal-over-water method on response allocation to the 2 temperature zones. Sleep deprivation decreased time spent on the cooled plate. Cumulative occupancy indicated differential effects for sleep deprivation with the rats preferring to remain on the hot side vs. the cold side, suggesting that sleep deprivation increased the nociceptive properties of the cold stimulus.
Harvey
Mark T
MT
John F. Kennedy Center, Box 40 Peabody College, Vanderbilt University, Nashville, TN 37203, USA.
Kline
Robert H
RH
May
Michael E
ME
Roberts
A Celeste
AC
Valdovinos
Maria G
MG
Wiley
Ronald G
RG
Kennedy
Craig H
CH
D000818:Animals; D003080:Cold Temperature / Q000009:adverse effects; D006358:Hot Temperature / Q000009:adverse effects; D008297:Male; D009413:Nerve Fibers, Myelinated / Q000502:physiology; D036421:Nerve Fibers, Unmyelinated / Q000502:physiology; D009619:Nociceptors / Q000502:physiology; D010146:Pain / Q000503:physiopathology*; D010147:Pain Measurement / Q000379:methods*; D017288:Pain Threshold / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011930:Reaction Time / Q000502:physiology; D012892:Sleep Deprivation / Q000503:physiopathology*; D013697:Thermosensing / Q000502:physiology*