Decreased chaperone activity of alpha-crystallins in naphthalene-induced cataract possibly results from C-terminal truncation.
Naphthalene-induced cataract has been extensively used to test potential anticataract drugs. Because the morphology as well as the toxic manifestations of naphthalene-induced cataract are reported to be similar to that of age-related cataract, naphthalene cataractogenesis in rats has been used as a valuable animal model to study the aetiology of age-related cataract in humans. This study aimed to determine whether the molecular chaperone activity of the alpha-crystallins was altered in naphthalene-induced cataract, and to clarify the possible mechanism for these changes. The data showed that the chaperone activity of the alpha-crystallins decreased in naphthalene-induced cataract. By mass spectrometry, C-terminal truncation of 16 amino acids and other post-translational modifications such as acetylation, phosphorylation, oxidation and carbamylation of the alpha-crystallins were detected. Furthermore, the results suggested that, at the proteomics level, naphthalene-induced cataract is a valuable animal model for the study of age-related cataract in humans.
Chen
Y
Y
Department of Ophthalmology, Eye and ENT Hospital of Fudan University, Shanghai, China.
Yi
L
L
Yan
G Q
GQ
Jang
Y X
YX
Fang
Y W
YW
Wu
X H
XH
Zhou
X W
XW
Wei
L M
LM
D000818:Animals; D002386:Cataract / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005978:Glutathione / Q000378:metabolism; D007908:Lens, Crystalline / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D018832:Molecular Chaperones / Q000378:metabolism*; D009281:Naphthalenes / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects; D010449:Peptide Mapping; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D038201:alpha-Crystallins / Q000737:chemistry / Q000378:metabolism*
D018832:Molecular Chaperones; D009281:Naphthalenes; D017382:Reactive Oxygen Species; D038201:alpha-Crystallins; C031721:naphthalene; D005978:Glutathione