title: The DOF transcription factor Dof5.1 influences leaf axial patterning by promoting Revoluta transcription in Arabidopsis.
abstract: Dof proteins are transcription factors that have a conserved single zinc finger DNA-binding domain. In this study, we isolated an activation tagging mutant Dof5.1-D exhibiting an upward-curling leaf phenotype due to enhanced expression of the REV gene that is required for establishing adaxial-abaxial polarity. Dof5.1-D plants also had reduced transcript levels for IAA6 and IAA19 genes, indicating an altered auxin biosynthesis in Dof5.1-D. An electrophoretic mobility shift assay using the Dof5.1 DNA-binding motif and the REV promoter region indicated that the DNA-binding domain of Dof5.1 binds to a TAAAGT motif located in the 5'-distal promoter region of the REV promoter. Further, transient and chromatin immunoprecipitation assays verified binding activity of the Dof5.1 DNA-binding motif with the REV promoter. Consistent with binding assays, constitutive over-expression of the Dof5.1 DNA-binding domain in wild-type plants caused a downward-curling phenotype, whereas crossing Dof5.1-D to a rev mutant reverted the upward-curling phenotype of the Dof5.1-D mutant leaf to the wild-type. These results suggest that the Dof5.1 protein directly binds to the REV promoter and thereby regulates adaxial-abaxial polarity.
authors.affiliation: Division of Bioscience and Bioinformatics, Myongji University, Yongin, Kyunggi-do 449-728, South Korea.
authors.forename: Hyung-Sae
authors.initials: HS
authors.lastname: Kim
authors.forename: Sung Jin
authors.initials: SJ
authors.lastname: Kim
authors.forename: Nazia
authors.initials: N
authors.lastname: Abbasi
authors.forename: Ray A
authors.initials: RA
authors.lastname: Bressan
authors.forename: Dae-Jin
authors.initials: DJ
authors.lastname: Yun
authors.forename: Sang-Dong
authors.initials: SD
authors.lastname: Yoo
authors.forename: Suk-Yun
authors.initials: SY
authors.lastname: Kwon
authors.forename: Sang-Bong
authors.initials: SB
authors.lastname: Choi
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D007210:Indoleacetic Acids / Q000378:metabolism; D016254:Mutagenesis, Insertional; D009154:Mutation; D018515:Plant Leaves / Q000254:growth & development*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D011401:Promoter Regions, Genetic; D054730:Protein Interaction Domains and Motifs; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D029681:Arabidopsis Proteins; D018398:Homeodomain Proteins; C402597:IFL1 protein, Arabidopsis; D007210:Indoleacetic Acids; D014157:Transcription Factors