title: Scaffold percolative efficiency: in vitro evaluation of the structural criterion for electrospun mats.
abstract: Fibrous scaffolds of engineered structures can be chosen as promising porous environments when an approved criterion validates their applicability for a specific medical purpose. For such biomaterials, this paper sought to investigate various structural characteristics in order to determine whether they are appropriate descriptors. A number of poly(3-hydroxybutyrate) scaffolds were electrospun; each of which possessed a distinguished architecture when their material and processing conditions were altered. Subsequent culture of mouse fibroblast cells (L929) was carried out to evaluate the cells viability on each scaffold after their attachment for 24 h and proliferation for 48 and 72 h. The scaffolds' porosity, pores number, pores size and distribution were quantified and none could establish a relationship with the viability results. Virtual reconstruction of the mats introduced an authentic criterion, "Scaffold Percolative Efficiency" (SPE), with which the above descriptors were addressed collectively. It was hypothesized to be able to quantify the efficacy of fibrous scaffolds by considering the integration of porosity and interconnectivity of the pores. There was a correlation of 80% as a good agreement between the SPE values and the spectrophotometer absorbance of viable cells; a viability of more than 350% in comparison to that of the controls.
authors.affiliation: Department of Textile Engineering, Isfahan University of Technology, 84156-83111 Isfahan, Iran.
authors.forename: Ashkan
authors.initials: A
authors.lastname: Heidarkhan Tehrani
authors.forename: Ali
authors.initials: A
authors.lastname: Zadhoush
authors.forename: Saeed
authors.initials: S
authors.lastname: Karbasi
authors.forename: Hojjat
authors.initials: H
authors.lastname: Sadeghi-Aliabadi
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004526:Efficiency; D004595:Electroplating / Q000295:instrumentation / Q000379:methods; D005347:Fibroblasts / Q000187:drug effects / Q000502:physiology; D005374:Filtration; D008422:Materials Testing; D051379:Mice; D008954:Models, Biological; D011108:Polymers / Q000737:chemistry* / Q000494:pharmacology; D016062:Porosity; D013499:Surface Properties; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D011108:Polymers
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