title: Relationship between nonstructural protein 1 detection and plasma virus load in Dengue patients.
abstract: We report data from a prospective observational study performed in Martinique during a co-epidemic of dengue virus serotype 2 (DENV-2) and serotype 4 (DENV-4). Among 70 serum samples from patients with DENV-2 (n = 21) or DENV-4 (n = 49) infections, 47 (67.1%) were positive for dengue nonstructural protein 1 (NS1). Antigenemia correlated with plasma virus load and was independent of immune status and the time of sampling. Increased viremia 4-6 days after onset of illness was associated with NS1 positivity, secondary infection, and severe disease. Testing for NS1 could help identify the potentially most severely ill patients during the critical phase of dengue.
authors.affiliation: Centre Hospitalier Universitaire, Fort-de-France, Martinique. laurent.thomas@chu-fortdefrance.fr
authors.forename: Laurent
authors.initials: L
authors.lastname: Thomas
authors.forename: Fatiha
authors.initials: F
authors.lastname: Najioullah
authors.forename: Olivier
authors.initials: O
authors.lastname: Verlaeten
authors.forename: Jenny
authors.initials: J
authors.lastname: Martial
authors.forename: Ségolène
authors.initials: S
authors.lastname: Brichler
authors.forename: Stéphane
authors.initials: S
authors.lastname: Kaidomar
authors.forename: Victor
authors.initials: V
authors.lastname: Moravie
authors.forename: André
authors.initials: A
authors.lastname: Cabié
authors.forename: Raymond
authors.initials: R
authors.lastname: Césaire
mesh_terms: D003715:Dengue / Q000378:metabolism* / Q000821:virology; D003716:Dengue Virus / Q000302:isolation & purification*; D006801:Humans; D019562:Viral Load; D017361:Viral Nonstructural Proteins / Q000378:metabolism*
chemical_list: D017361:Viral Nonstructural Proteins
references.citation: PLoS Negl Trop Dis. 2008 Aug 20;2(8):e280
references.pmid: 18714359
references.citation: J Infect Dis. 2006 Apr 15;193(8):1078-88
references.pmid: 16544248
references.citation: PLoS Negl Trop Dis. 2009;3(1):e360
references.pmid: 19156192
references.citation: J Infect Dis. 2000 Jan;181(1):2-9
references.pmid: 10608744
references.citation: Am J Trop Med Hyg. 2008 Jun;78(6):990-8
references.pmid: 18541782
references.citation: Clin Microbiol Rev. 2009 Oct;22(4):564-81
references.pmid: 19822889
references.citation: J Infect Dis. 2002 Oct 15;186(8):1165-8
references.pmid: 12355369
references.citation: J Clin Microbiol. 2003 Sep;41(9):4154-9
references.pmid: 12958240
references.citation: J Clin Microbiol. 1992 Mar;30(3):545-51
references.pmid: 1372617
references.citation: Clin Infect Dis. 2006 Oct 15;43(8):1023-30
references.pmid: 16983615
references.citation: J Clin Microbiol. 2000 Mar;38(3):1053-7
references.pmid: 10698995
references.citation: J Clin Virol. 2007 Jul;39(3):194-8
references.pmid: 17521960
references.citation: Diagn Microbiol Infect Dis. 2008 Apr;60(4):387-91
references.pmid: 18191361
references.citation: Clin Diagn Lab Immunol. 2003 Jul;10(4):622-30
references.pmid: 12853395
references.citation: Arch Virol. 1997;142(5):897-916
references.pmid: 9191856
references.citation: Science. 1988 Jan 29;239(4839):476-81
references.pmid: 3277268
references.citation: J Virol. 1999 Jul;73(7):6104-10
references.pmid: 10364366