title: Expression of RAD51, BRCA1 and P53 does not correlate with cellular radiosensitivity of normal human fibroblasts.
abstract: OBJECTIVE
  To evaluate the potential role of key DNA repair proteins in the sensitivity of normal human fibroblasts to ionising radiations.
  
  
  METHODS
  Radiosensitivity of six human fibroblast strains established from skin biopsies of women who had undergone conservative breast surgery and received a curative breast conserving radiotherapy was measured by colony-formation assay. The expression level of RAD51, BRCA1 and p53 proteins were studied using western blot analysis.
  
  
  RESULTS
  The six fibroblast strains represent a typical spectrum of normal human radiosensitivity with the surviving fraction measured for a dose of 3.5 Gy (SF3.5) ranging from 0.21 to 0.40. We found that these differences in cell survival did not correlate with the expression of RAD51, BRCA1 nor p53 in the tested normal human fibroblast strains.
  
  
  CONCLUSIONS
  We conclude that measurement of protein expression of the three tested genes (RAD51, BRCA1 and p53) did not reflect sensitivity of normal fibroblasts to IR.
authors.affiliation: Biochemistry and Molecular Biology Unit, Cancer Biology Department, National Cancer Institute, Cairo University, Cairo, Egypt. ekramsaleh@hotmail.com
authors.forename: E M Y
authors.initials: EM
authors.lastname: Saleh
authors.forename: R A E
authors.initials: RA
authors.lastname: El-Awady
mesh_terms: D019313:BRCA1 Protein / Q000378:metabolism*; D015153:Blotting, Western; D002470:Cell Survival; D004260:DNA Repair / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005347:Fibroblasts / Q000378:metabolism*; D016158:Genes, p53 / Q000502:physiology*; D006801:Humans; D015151:Immunoblotting; D010766:Phosphorylation; D051135:Rad51 Recombinase / Q000378:metabolism*; D011836:Radiation Tolerance
chemical_list: D019313:BRCA1 Protein; D051135:Rad51 Recombinase
references.citation: Int J Radiat Biol. 2005 Jul;81(7):501-8
references.pmid: 16263653
references.citation: Biochem Soc Trans. 2001 May;29(Pt 2):196-201
references.pmid: 11356153
references.citation: Br J Cancer. 2004 Jun 14;90(12):2356-63
references.pmid: 15150571
references.citation: EMBO J. 1998 Jan 15;17(2):598-608
references.pmid: 9430650
references.citation: Br J Cancer. 2005 Mar 28;92(6):1089-97
references.pmid: 15785736
references.citation: Nat Cell Biol. 2000 Oct;2(10):757-61
references.pmid: 11025669
references.citation: Int J Radiat Oncol Biol Phys. 2000 Nov 1;48(4):959-65
references.pmid: 11072151
references.citation: Nature. 1992 Jul 2;358(6381):15-6
references.pmid: 1614522
references.citation: Eur J Biochem. 2001 May;268(10):2764-72
references.pmid: 11358490
references.citation: Br J Cancer. 2003 Apr 22;88(8):1251-5
references.pmid: 12698192
references.citation: Carcinogenesis. 2002 May;23(5):687-96
references.pmid: 12016139
references.citation: Genes Dev. 1997 May 15;11(10):1242-52
references.pmid: 9171369
references.citation: Proc Natl Acad Sci U S A. 2001 Jul 17;98(15):8388-94
references.pmid: 11459980
references.citation: Nat Rev Cancer. 2003 Feb;3(2):117-29
references.pmid: 12563311
references.citation: Br J Cancer. 2003 Aug 4;89(3):593-601
references.pmid: 12888835
references.citation: Int J Radiat Biol. 2004 Jan;80(1):53-63
references.pmid: 14761850
references.citation: Nat Genet. 2001 Jul;28(3):266-71
references.pmid: 11431698
references.citation: Cell. 2002 Jan 25;108(2):171-82
references.pmid: 11832208
references.citation: J Clin Oncol. 2000 Oct 1;18(19):3360-9
references.pmid: 11013276
references.citation: Cancer Res. 2004 Oct 1;64(19):7065-72
references.pmid: 15466201
references.citation: Int J Radiat Biol. 1997 Feb;71(2):225-9
references.pmid: 9120358
references.citation: Mol Cell Biol. 2000 Sep;20(17):6476-82
references.pmid: 10938124
references.citation: DNA Repair (Amst). 2003 Sep 18;2(9):1015-28
references.pmid: 12967658
references.citation: DNA Repair (Amst). 2004 Aug-Sep;3(8-9):1237-43
references.pmid: 15279812
references.citation: Oncogene. 2002 Dec 16;21(58):8981-93
references.pmid: 12483514
references.citation: Nat Genet. 1997 Dec;17(4):423-30
references.pmid: 9398843
references.citation: Oncogene. 1997 Mar 6;14(9):1117-22
references.pmid: 9070661
references.citation: Cancer Res. 2001 Jun 15;61(12):4842-50
references.pmid: 11406561
references.citation: Mol Cell Biol. 1998 Sep;18(9):5332-42
references.pmid: 9710617
references.citation: Oncogene. 2000 Feb 3;19(5):632-9
references.pmid: 10698508
references.citation: Int J Radiat Oncol Biol Phys. 1996 Dec 1;36(5):1065-75
references.pmid: 8985028
references.citation: Nature. 2000 Jul 13;406(6792):210-5
references.pmid: 10910365
references.citation: Mol Cell Biol. 2001 Apr;21(8):2858-66
references.pmid: 11283264
references.citation: Genes Dev. 2000 Apr 15;14(8):927-39
references.pmid: 10783165
references.citation: Br J Cancer. 1999 Mar;79(7-8):1037-41
references.pmid: 10098733
references.citation: Oncogene. 1997 Apr 17;14(15):1847-57
references.pmid: 9150391
references.citation: Cell. 1996 Jun 28;85(7):1009-23
references.pmid: 8674108
references.citation: Mol Cell. 1998 Feb;1(3):347-57
references.pmid: 9660919
references.citation: J Cell Sci. 2002 Jan 1;115(Pt 1):153-64
references.pmid: 11801733
references.citation: Int J Radiat Oncol Biol Phys. 2001 Aug 1;50(5):1323-31
references.pmid: 11483345
references.citation: Semin Radiat Oncol. 1994 Apr;4(2):68-80
references.pmid: 10717093
references.citation: Mol Cell Biol. 1994 Sep;14(9):6264-77
references.pmid: 8065358
references.citation: Nature. 2000 Nov 16;408(6810):307-10
references.pmid: 11099028
references.citation: Cancer Res. 2003 Jul 15;63(14):4074-81
references.pmid: 12874009
references.citation: Int J Cancer. 2000 Mar 15;85(6):845-9
references.pmid: 10709106