title: Metabolically engineered male sterility in rapeseed (Brassica napus L.).
abstract: Male sterility is of special interest as a mechanism allowing hybrid breeding, especially in important crops such as rapeseed (Brassica napus). Male sterile plants are also suggested to be used as a biological safety method to prevent the spread of transgenes, a risk that is high in the case of rapeseed due to the mode of pollination, out-crossing by wind or insects, and the presence of related, cross-pollinating species in the surrounding ecosystem in Europe. Different natural occurring male sterilities and alloplasmic forms have been tried to be used in rapeseed with more or less success. Due to the difficulties and limitations with these systems, we present a biotechnological alternative: a metabolically engineered male sterility caused by interference with anther-specific cell wall-bound invertase. This is an essential enzyme for carbohydrate supply of the symplastically isolated pollen. The activity of this enzyme is reduced either by antisense interference or by expressing an invertase inhibitor under control of the anther-specific promoter of the invertase with the consequence of a strong decrease of pollen germination ability.
authors.affiliation: Lehrstuhl für Pharmazeutische Biologie, Julius von Sachs Institut, Universität Würzburg, Julius von Sachs Platz 2, 97082, Würzburg, Germany. thomas.engelke@biozentrum.uni-wuerzburg.de
authors.forename: Thomas
authors.initials: T
authors.lastname: Engelke
authors.forename: J
authors.initials: J
authors.lastname: Hirsche
authors.forename: T
authors.initials: T
authors.lastname: Roitsch
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000201:enzymology / Q000235:genetics; D029688:Brassica napus / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant / Q000235:genetics; D017930:Genes, Reporter; D005818:Genetic Engineering / Q000379:methods*; D018525:Germination / Q000187:drug effects; D005966:Glucuronidase / Q000378:metabolism; D005810:Multigene Family / Q000235:genetics; D009928:Organ Specificity / Q000187:drug effects / Q000235:genetics; D010802:Phylogeny; D051479:Plant Infertility / Q000187:drug effects / Q000235:genetics*; D011058:Pollen / Q000187:drug effects / Q000254:growth & development; D011401:Promoter Regions, Genetic / Q000235:genetics; D034622:RNA Interference / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D013045:Species Specificity; D043324:beta-Fructofuranosidase / Q000037:antagonists & inhibitors / Q000235:genetics
chemical_list: D004791:Enzyme Inhibitors; D012333:RNA, Messenger; D043324:beta-Fructofuranosidase; D005966:Glucuronidase
references.citation: Plant J. 2007 Nov;52(3):570-82
references.pmid: 17764500
references.citation: Plant J. 1999 Dec;20(6):707-11
references.pmid: 10652142
references.citation: J Exp Bot. 2005 Jan;56(409):179-90
references.pmid: 15533880
references.citation: J Mol Evol. 2005 May;60(5):615-34
references.pmid: 15983871
references.citation: Planta. 2007 Apr;225(5):1193-203
references.pmid: 17086397
references.citation: Plant Physiol. 1999 Sep;121(1):1-8
references.pmid: 10482654
references.citation: Gene. 1997 Sep 15;197(1-2):239-51
references.pmid: 9332372
references.citation: Plant Cell Rep. 2003 Feb;21(6):599-604
references.pmid: 12789436
references.citation: Plant J. 2003 Dec;36(5):697-710
references.pmid: 14617070
references.citation: Theor Appl Genet. 2009 Jan;118(2):235-45
references.pmid: 18825361
references.citation: Nucleic Acids Res. 1993 Apr 25;21(8):1903-9
references.pmid: 8388101
references.citation: Plant Mol Biol. 1999 Dec;41(6):741-51
references.pmid: 10737139
references.citation: Bioinformatics. 2007 Nov 1;23(21):2947-8
references.pmid: 17846036
references.citation: Planta. 1998 Dec;207(2):259-65
references.pmid: 9951726
references.citation: Plant J. 2003 Jul;35(2):262-72
references.pmid: 12848830
references.citation: Plant Cell Rep. 2005 Jun;24(4):225-36
references.pmid: 15759120
references.citation: Theor Appl Genet. 2008 Feb;116(3):355-62
references.pmid: 18034224
references.citation: Planta. 2008 Mar;227(4):795-807
references.pmid: 18034262
references.citation: Plant Physiol. 2003 Feb;131(2):603-9
references.pmid: 12586884
references.citation: Trends Plant Sci. 1999 Oct;4(10):401-407
references.pmid: 10498964
references.citation: J Exp Bot. 2003 Jan;54(382):525-31
references.pmid: 12508063
references.citation: Theor Appl Genet. 2006 Dec;114(1):93-9
references.pmid: 17036218
references.citation: Curr Genet. 1997 Apr;31(4):325-35
references.pmid: 9108140
references.citation: Mol Gen Genet. 1992 Nov;235(2-3):340-8
references.pmid: 1281515
references.citation: Proc Natl Acad Sci U S A. 2001 May 22;98(11):6522-7
references.pmid: 11371651
references.citation: Theor Appl Genet. 1983 May;65(3):231-8
references.pmid: 24263420
references.citation: Plant Physiol. 1995 May;108(1):285-94
references.pmid: 7784506
references.citation: Adv Bioinformatics. 2008;2008:420747
references.pmid: 19956698
references.citation: Nucleic Acids Res. 1997 Sep 1;25(17):3389-402
references.pmid: 9254694
references.citation: Proc Natl Acad Sci U S A. 1998 Aug 18;95(17):10032-7
references.pmid: 9707595
references.citation: Plant Physiol. 1997 Sep;115(1):273-82
references.pmid: 9306701
references.citation: Plant Physiol. 2007 Nov;145(3):616-25
references.pmid: 17873089
references.citation: Mol Gen Genet. 1994 Jun 3;243(5):540-7
references.pmid: 8208245
references.citation: Plant Mol Biol. 1995 Apr;28(1):189-94
references.pmid: 7787183
references.citation: Planta. 2003 Apr;216(6):951-60
references.pmid: 12687362
references.citation: Plant Cell. 1991 Dec;3(12):1349-62
references.pmid: 1840901
references.citation: J Exp Bot. 2010 Jun;61(10):2693-706
references.pmid: 20427415