title: Bartonella species, feline herpesvirus-1, and Toxoplasma gondii PCR assay results from blood and aqueous humor samples from 104 cats with naturally occurring endogenous uveitis.
abstract: Toxoplasma gondii, Bartonella henselae and feline herpesvirus-1 (FHV-1) have been implicated as causative agents in feline uveitis. The usefulness of serum and aqueous humor (AH) antibody testing for these agents is limited as antibodies can be detected in both healthy cats and cats with uveitis. Very few studies using polymerase chain reaction (PCR) assays to amplify organism DNA from samples from cats with uveitis have been performed. In this study, assays to detect T gondii antibodies, feline leukemia virus antigen, feline immunodeficiency virus antibody, and Bartonella species antibodies were performed on serum and PCR assays for amplification of T gondii, Bartonella species, and FHV-1 DNA were performed on blood and AH samples from 104 cats with endogenous uveitis and 19 healthy cats. Results suggest the addition of the PCR assay to the diagnostic work-up for cats with uveitis will increase the detection of T gondii and FHV-1; however, the diagnostic usefulness of these additional data is not clear.
authors.affiliation: Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO, USA. ceep@colostate.edu
authors.forename: Cynthia C
authors.initials: CC
authors.lastname: Powell
authors.forename: Carey L
authors.initials: CL
authors.lastname: McInnis
authors.forename: Jennifer P
authors.initials: JP
authors.lastname: Fontenelle
authors.forename: Michael R
authors.initials: MR
authors.lastname: Lappin
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis / Q000097:blood; D000913:Antibodies, Protozoan / Q000032:analysis / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral / Q000097:blood; D001082:Aqueous Humor / Q000276:immunology; D018416:Bartonella henselae / Q000276:immunology / Q000302:isolation & purification*; D002371:Cat Diseases / Q000382:microbiology / Q000469:parasitology / Q000821:virology; D002415:Cats; D004279:DNA, Viral / Q000032:analysis / Q000097:blood; D005260:Female; D006564:Herpesviridae / Q000235:genetics / Q000302:isolation & purification*; D016089:Immunodeficiency Virus, Feline / Q000276:immunology; D005256:Leukemia Virus, Feline / Q000276:immunology; D008297:Male; D016133:Polymerase Chain Reaction / Q000662:veterinary; D014122:Toxoplasma / Q000276:immunology / Q000302:isolation & purification*; D014605:Uveitis / Q000382:microbiology / Q000469:parasitology / Q000662:veterinary* / Q000821:virology
chemical_list: D000907:Antibodies, Bacterial; D000913:Antibodies, Protozoan; D000914:Antibodies, Viral; D000956:Antigens, Viral; D004279:DNA, Viral
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