title: iPSC lines that do not silence the expression of the ectopic reprogramming factors may display enhanced propensity to genomic instability.
abstract: Here, we provide data suggesting that the absence of silencing of the ectopic reprogramming factors used to reprogram somatic cells to induced pluripotent stem cells (iPSCs) may predispose iPSCs to genomic instability. We encourage stem cell scientists to undertake an extensive characterization and standardization of much larger cohorts of iPSC lines in order to set up rigorous criteria to define safe and stable bona fide iPSCs.
authors.affiliation: Andalusian Stem Cell Bank, Centro de Investigacion Biomedica, CSJA-UGR, Granada, Spain.
authors.forename: Veronica
authors.initials: V
authors.lastname: Ramos-Mejia
authors.forename: Martin
authors.initials: M
authors.lastname: Muñoz-Lopez
authors.forename: Jose L
authors.initials: JL
authors.lastname: Garcia-Perez
authors.forename: Pablo
authors.initials: P
authors.lastname: Menendez
mesh_terms: D065150:Cellular Reprogramming; D020868:Gene Silencing; D042822:Genomic Instability; D057026:Induced Pluripotent Stem Cells / Q000378:metabolism*; D007621:Karyotyping; D016086:Lentivirus / Q000235:genetics