title: Endothelial NOS-derived nitric oxide prevents injury resulting from reoxygenation in the hypoxic lung.
abstract: To date, the role that NO derived from endothelial NO synthase (eNOS) plays in the development of the injuries occurring under hypoxia/reoxygenation (H/R) in the lung remains unknown and thus constitutes the subject of the present work. A follow-up study was conducted in Wistar rats submitted to H/R (hypoxia for 30 min; reoxygenation of 0 h, 48 h and 5 days), with or without prior treatment using the eNOS inhibitor L-NIO (20 mg/kg). Lipid peroxidation, apoptosis, protein nitration and NO production (NOx) were analysed. The results showed that L-NIO administration lowered NOx levels in all the experimental groups. Contrarily, the lipid peroxidation level and the percentage of apoptotic cells rose, implying that eNOS-derived NO may have a protective effect against the injuries occurring during H/R in the lung. These findings could open the possibility of future studies to design new therapies for this type of hypoxia based on NO-pharmacology.
authors.affiliation: Department of Experimental Biology, University of Jaén, Spain.
authors.forename: Alma
authors.initials: A
authors.lastname: Rus
authors.forename: Francisco
authors.initials: F
authors.lastname: Molina
authors.forename: Ma Angeles
authors.initials: MA
authors.lastname: Peinado
authors.forename: Ma Luisa
authors.initials: ML
authors.lastname: del Moral
mesh_terms: D000818:Animals; D019610:Cytoprotection / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D000860:Hypoxia / Q000150:complications / Q000378:metabolism*; D020287:In Situ Nick-End Labeling; D008168:Lung / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism / Q000494:pharmacology / Q000502:physiology*; D052250:Nitric Oxide Synthase Type III / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000502:physiology*; D009952:Ornithine / Q000031:analogs & derivatives / Q000494:pharmacology; D010100:Oxygen / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000209:etiology / Q000517:prevention & control*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014443:Tyrosine / Q000031:analogs & derivatives / Q000378:metabolism
chemical_list: D004791:Enzyme Inhibitors; D017392:Thiobarbituric Acid Reactive Substances; D009569:Nitric Oxide; C002744:3-nitrotyrosine; C065027:N(G)-iminoethylornithine; D014443:Tyrosine; D009952:Ornithine; D052250:Nitric Oxide Synthase Type III; C496316:Nos3 protein, rat; D010100:Oxygen