title: Temperature modulation of integrin-mediated cell adhesion.
abstract: In response to external stimuli, cells modulate their adhesive state by regulating the number and intrinsic affinity of receptor/ligand bonds. A number of studies have shown that cell adhesion is dramatically reduced at room or lower temperatures as compared with physiological temperature. However, the underlying mechanism that modulates adhesion is still unclear. Here, we investigated the adhesion of the monocytic cell line THP-1 to a surface coated with intercellular adhesion molecule-1 (ICAM-1) as a function of temperature. THP-1 cells express the integrin lymphocyte function-associated antigen-1 (LFA-1), a receptor for ICAM-1. Direct force measurements of cell adhesion and cell elasticity were carried out by atomic force microscopy. Force measurements revealed an increase of the work of de-adhesion with temperature that was coupled to a gradual decrease in cellular stiffness. Of interest, single-molecule measurements revealed that the rupture force of the LFA-1/ICAM-1 complex decreased with temperature. A detailed analysis of the force curves indicated that temperature-modulated cell adhesion was mainly due to the enhanced ability of cells to deform and to form a greater number of longer membrane tethers at physiological temperatures. Together, these results emphasize the importance of cell mechanics and membrane-cytoskeleton interaction on the modulation of cell adhesion.
authors.affiliation: Department of Physiology and Biophysics, University of Miami, Miller School of Medicine, Miami, Florida, USA. felix.rico@curie.fr
authors.forename: Félix
authors.initials: F
authors.lastname: Rico
authors.forename: Calvin
authors.initials: C
authors.lastname: Chu
authors.forename: Midhat H
authors.initials: MH
authors.lastname: Abdulreda
authors.forename: Yujing
authors.initials: Y
authors.lastname: Qin
authors.forename: Vincent T
authors.initials: VT
authors.lastname: Moy
mesh_terms: D000199:Actins / Q000378:metabolism; D002448:Cell Adhesion; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D002470:Cell Survival; D003599:Cytoskeleton / Q000378:metabolism; D004548:Elasticity; D006801:Humans; D016023:Integrins / Q000378:metabolism*; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D016169:Lymphocyte Function-Associated Antigen-1 / Q000378:metabolism; D018625:Microscopy, Atomic Force; D013696:Temperature
chemical_list: D000199:Actins; D016023:Integrins; D016169:Lymphocyte Function-Associated Antigen-1; D018799:Intercellular Adhesion Molecule-1
references.citation: Soft Matter. 2008 Jun 20;4(7):1373-1387
references.pmid: 32907100
references.citation: Proc Natl Acad Sci U S A. 2008 Oct 14;105(41):15755-60
references.pmid: 18852468
references.citation: Nat Rev Drug Discov. 2003 Sep;2(9):703-16
references.pmid: 12951577
references.citation: J Biol Chem. 2001 Apr 20;276(16):13283-8
references.pmid: 11278674
references.citation: Cell. 1987 Dec 4;51(5):813-9
references.pmid: 3315233
references.citation: J Cell Biol. 1993 Jan;120(2):545-56
references.pmid: 7678422
references.citation: Cell. 2002 Sep 20;110(6):673-87
references.pmid: 12297042
references.citation: Biophys J. 2007 Oct 15;93(8):2923-33
references.pmid: 17586566
references.citation: Am J Physiol Cell Physiol. 2002 Mar;282(3):C606-16
references.pmid: 11832346
references.citation: Proc Natl Acad Sci U S A. 2005 Nov 15;102(46):16638-43
references.pmid: 16269547
references.citation: Biorheology. 2003;40(5):567-76
references.pmid: 12897422
references.citation: Science. 2009 Jan 30;323(5914):642-4
references.pmid: 19179533
references.citation: J Cell Physiol. 1977 Aug;92(2):209-20
references.pmid: 881433
references.citation: J Cell Biol. 1976 Nov;71(2):670-4
references.pmid: 993265
references.citation: Phys Biol. 2009 Jul 01;6(2):025009
references.pmid: 19571363
references.citation: J Mol Recognit. 2007 Nov-Dec;20(6):495-501
references.pmid: 17902095
references.citation: Curr Biol. 2007 Apr 17;17(8):694-9
references.pmid: 17379524
references.citation: Biophys J. 2008 Dec;95(11):5439-48
references.pmid: 18757559
references.citation: Pflugers Arch. 1996 Sep;432(5):753-9
references.pmid: 8772123
references.citation: J Cell Sci. 2007 Jul 1;120(Pt 13):2223-31
references.pmid: 17550968
references.citation: Proc Natl Acad Sci U S A. 2006 May 16;103(20):7660-3
references.pmid: 16679410
references.citation: J Cell Sci. 2005 Sep 15;118(Pt 18):4199-206
references.pmid: 16155253
references.citation: Nature. 1990 Aug 2;346(6283):425-34
references.pmid: 1974032
references.citation: Biophys J. 2002 Oct;83(4):2270-9
references.pmid: 12324444
references.citation: Proc Natl Acad Sci U S A. 2001 May 22;98(11):6009-14
references.pmid: 11353828
references.citation: Proc Natl Acad Sci U S A. 1998 Jun 9;95(12):6797-802
references.pmid: 9618492
references.citation: J Cell Biol. 1990 Jun;110(6):2157-65
references.pmid: 1693625
references.citation: Methods Cell Biol. 2007;83:373-96
references.pmid: 17613317
references.citation: Biophys J. 2006 Nov 1;91(9):3508-18
references.pmid: 16891365
references.citation: Biophys J. 1979 Apr;26(1):115-31
references.pmid: 262408
references.citation: Curr Opin Cell Biol. 2003 Oct;15(5):547-56
references.pmid: 14519389
references.citation: J Mol Biol. 2005 Nov 25;354(2):497-503
references.pmid: 16246362
references.citation: J Cell Biol. 1994 Dec;127(5):1485-95
references.pmid: 7525609
references.citation: Methods Cell Biol. 2007;83:347-72
references.pmid: 17613316
references.citation: Biophys J. 2001 Feb;80(2):668-82
references.pmid: 11159435
references.citation: J Cell Physiol. 1979 Mar;98(3):483-9
references.pmid: 556177
references.citation: Biophys J. 2007 Dec 1;93(11):4041-52
references.pmid: 17704170
references.citation: Nat Methods. 2009 May;6(5):383-7
references.pmid: 19363493
references.citation: Annu Rev Biophys Biomol Struct. 2006;35:417-34
references.pmid: 16689643
references.citation: Nat Cell Biol. 2008 Apr;10(4):429-36
references.pmid: 18364700
references.citation: Proc Natl Acad Sci U S A. 1982 Sep;79(17):5327-31
references.pmid: 6957866
references.citation: Biophys J. 2006 Apr 15;90(8):2994-3003
references.pmid: 16443660
references.citation: Science. 2009 Jan 30;323(5914):638-41
references.pmid: 19179532
references.citation: Biophys J. 1995 Mar;68(3):988-96
references.pmid: 7756561
references.citation: Proc Natl Acad Sci U S A. 1999 Jan 19;96(2):471-6
references.pmid: 9892657
references.citation: Biophys J. 2005 Mar;88(3):2288-98
references.pmid: 15653718
references.citation: J Mol Recognit. 2007 Nov-Dec;20(6):459-66
references.pmid: 17891755
references.citation: Biophys J. 2005 Jul;89(1):187-200
references.pmid: 15879471
references.citation: Blood. 1975 Oct;46(4):611-24
references.pmid: 1174695
references.citation: Biomacromolecules. 2006 Nov;7(11):3188-95
references.pmid: 17096550
references.citation: Biophys J. 2004 Feb;86(2):1223-33
references.pmid: 14747356
references.citation: J Lipid Res. 2009 May;50(5):957-65
references.pmid: 19114730
references.citation: J Cell Sci. 2003 Jun 15;116(Pt 12):2531-9
references.pmid: 12734401
references.citation: Colloids Surf B Biointerfaces. 2004 Mar 15;34(2):141-6
references.pmid: 15261083