Conserved RNaseII domain protein functions in cytoplasmic mRNA decay and suppresses Arabidopsis decapping mutant phenotypes.
Both transcription and RNA decay are critical for normal gene regulation. Arabidopsis mutants with defects in VARICOSE (VCS), a decapping complex scaffold protein, lack mRNA decapping and 5'-to-3' decay. These mutants show either severe or suppressed phenotypes, depending on the Arabidopsis accession. Here, we show that the molecular basis for this variation is the SUPPRESSOR OF VARICOSE (SOV), a locus that encodes a conserved, cytoplasmically localized RRP44-like RNaseII-domain protein. In vivo RNA decay assays suggest that active forms of this protein carry out decay on mRNA substrates that overlap with those of the decapping complex. Members of this conserved gene family encode proteins lacking the PIN domain, suggesting that SOV is not a functional component of the RNA exosome.
Zhang
Weiping
W
Department of Biology, University of Utah, Salt Lake City, 84112-6517, USA.
Murphy
Caroline
C
Sieburth
Leslie E
LE
D017360:Arabidopsis / Q000235:genetics* / Q000502:physiology; D017124:Conserved Sequence; D003593:Cytoplasm / Q000201:enzymology* / Q000378:metabolism; D019143:Evolution, Molecular; D005095:Exoribonucleases / Q000737:chemistry / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics*
D012333:RNA, Messenger; D005095:Exoribonucleases; C022535:exoribonuclease II
Proc Natl Acad Sci U S A. 2000 Dec 5;97(25):13985-90
11106401
Plant Cell. 2007 May;19(5):1549-64
17513503
Nature. 2009 Sep 10;461(7261):225-9
19701183
Trends Genet. 2004 Oct;20(10):491-7
15363903
Plant J. 2006 Feb;45(4):616-29
16441352
Mol Cell. 2008 Mar 28;29(6):717-28
18374646
Plant J. 1996 Oct;10(4):733-6
8893548
Proc Natl Acad Sci U S A. 2002 Apr 30;99(9):5860-5
11972065
Science. 2007 Jul 20;317(5836):338-42
17641193
J Cell Biol. 2006 Mar 13;172(6):803-8
16520386
Curr Opin Plant Biol. 2009 Feb;12(1):96-102
18990607
Plant Cell. 2008 Oct;20(10):2571-85
18952771
Cell. 2006 Dec 15;127(6):1223-37
17174896
Cell. 2007 Dec 28;131(7):1340-53
18160042
Nat Struct Mol Biol. 2010 Feb;17(2):244-7
20118937
Plant J. 1998 Dec;16(6):735-43
10069079
Plant J. 2007 Jul;51(2):281-92
17559508
Nat Struct Mol Biol. 2007 Jan;14(1):15-22
17173052
Plant Cell. 2006 Dec;18(12):3386-98
17158604
J Cell Biol. 2004 Apr;165(1):31-40
15067023
Proc Natl Acad Sci U S A. 2007 Oct 23;104(43):16844-9
17942686
Nat Rev Mol Cell Biol. 2007 Feb;8(2):113-26
17245413
Proc Natl Acad Sci U S A. 2002 Aug 20;99(17):11513-8
12167669
Proc Natl Acad Sci U S A. 1997 May 27;94(11):5628-33
9159123
Science. 2004 Nov 5;306(5698):1046-8
15528448
Mol Cell Biol. 2010 Jan;30(2):460-9
19901075
Nat Struct Mol Biol. 2009 Jun;16(6):616-23
19430462
Development. 2003 Dec;130(26):6577-88
14660546
Nucleic Acids Res. 2008 Jul 1;36(Web Server issue):W465-9
18424797
Science. 2003 Aug 1;301(5633):653-7
12893945
Genes Dev. 1993 Aug;7(8):1632-43
8393418
Science. 2003 May 2;300(5620):805-8
12730603
Nucleic Acids Res. 2009 Mar;37(4):1127-40
19129231
Mol Cell Biol. 1993 Aug;13(8):4826-35
8336719
Mol Cell. 2005 Dec 22;20(6):905-15
16364915
Genome Res. 2008 Jun;18(6):918-29
18323538
Science. 2008 May 30;320(5880):1185-90
18483398
RNA. 2005 Dec;11(12):1795-802
16314453
Plant Cell. 2007 Nov;19(11):3418-36
18024567
Nature. 2006 Sep 7;443(7107):110-4
16957732
Nat Struct Mol Biol. 2009 Jan;16(1):56-62
19060898
Mol Cell Biol. 2007 Jun;27(11):3970-81
17403906