title: Adhesion through single peptide aptamers.
abstract: Aptamer and antibody mediated adhesion is central to biological function and is valuable in the engineering of "lab on a chip" devices. Single molecule force spectroscopy using optical tweezers enables direct nonequilibrium measurement of these noncovalent interactions for three peptide aptamers selected for glass, polystyrene, and carbon nanotubes. A comprehensive examination of the strong attachment between antifluorescein 4-4-20 and fluorescein was also carried out using the same assay. Bond lifetime, barrier width, and free energy of activation are extracted from unbinding histogram data using three single molecule pulling models. The evaluated aptamers appear to adhere stronger than the fluorescein antibody under no- and low-load conditions, yet weaker than antibodies at loads above ∼25 pN. Comparison to force spectroscopy data of other biological linkages shows the diversity of load dependent binding and provides insight into linkages used in biological processes and those designed for engineered systems.
authors.affiliation: Department of Mechanical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, United States.
authors.forename: Marie-Eve
authors.initials: ME
authors.lastname: Aubin-Tam
authors.forename: David C
authors.initials: DC
authors.lastname: Appleyard
authors.forename: Enrico
authors.initials: E
authors.lastname: Ferrari
authors.forename: Valeria
authors.initials: V
authors.lastname: Garbin
authors.forename: Oluwatimilehin O
authors.initials: OO
authors.lastname: Fadiran
authors.forename: Jacquelyn
authors.initials: J
authors.lastname: Kunkel
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Lang
mesh_terms: D052158:Aptamers, Peptide / Q000737:chemistry*; D001665:Binding Sites; D005898:Glass / Q000737:chemistry; D037742:Nanotubes, Carbon / Q000737:chemistry; D011137:Polystyrenes / Q000737:chemistry
chemical_list: D052158:Aptamers, Peptide; D037742:Nanotubes, Carbon; D011137:Polystyrenes
references.citation: Opt Express. 2008 Dec 8;16(25):20987-1003
references.pmid: 19065239
references.citation: J Chem Phys. 2009 May 7;130(17):171102
references.pmid: 19425762
references.citation: J Mol Biol. 1987 Aug 5;196(3):641-56
references.pmid: 3681970
references.citation: Biophys J. 1997 Apr;72(4):1541-55
references.pmid: 9083660
references.citation: Nanotechnology. 2008 Nov 19;19(46):465101
references.pmid: 21836232
references.citation: Langmuir. 2008 Feb 19;24(4):1194-203
references.pmid: 18198910
references.citation: Science. 1978 May 12;200(4342):618-27
references.pmid: 347575
references.citation: Anal Chem. 2009 Apr 15;81(8):3159-64
references.pmid: 19364143
references.citation: J Phys Chem B. 2007 Mar 1;111(8):1963-74
references.pmid: 17284065
references.citation: Nat Rev Drug Discov. 2006 Feb;5(2):123-32
references.pmid: 16518379
references.citation: Science. 1997 May 16;276(5315):1109-12
references.pmid: 9148804
references.citation: Nano Lett. 2006 Mar;6(3):515-9
references.pmid: 16522054
references.citation: Nature. 1999 Jan 7;397(6714):50-3
references.pmid: 9892352
references.citation: Biophys J. 2003 Jul;85(1):5-15
references.pmid: 12829459
references.citation: Biophys J. 2006 Aug 1;91(3):1069-77
references.pmid: 16648165
references.citation: Nat Methods. 2004 Nov;1(2):133-9
references.pmid: 15782176
references.citation: Nature. 1993 Oct 21;365(6448):721-7
references.pmid: 8413650
references.citation: Biophys J. 2002 Jul;83(1):491-501
references.pmid: 12080136
references.citation: FEBS Lett. 2006 Jan 23;580(2):505-9
references.pmid: 16388805
references.citation: Proc Natl Acad Sci U S A. 2008 Oct 14;105(41):15755-60
references.pmid: 18852468
references.citation: Nat Mater. 2005 Jun;4(6):496-502
references.pmid: 15895095
references.citation: Science. 2005 Sep 23;309(5743):2057-60
references.pmid: 16179479
references.citation: Biochemistry. 2000 Aug 22;39(33):10219-23
references.pmid: 10956011
references.citation: Angew Chem Int Ed Engl. 2009;48(15):2672-89
references.pmid: 19319884
references.citation: Phys Rev Lett. 2008 Apr 4;100(13):138302
references.pmid: 18518003
references.citation: Proc Natl Acad Sci U S A. 2000 Sep 26;97(20):10701-5
references.pmid: 10984501
references.citation: Nature. 2002 Jul 11;418(6894):252-8
references.pmid: 12110902
references.citation: Proc Natl Acad Sci U S A. 2000 Oct 10;97(21):11262-7
references.pmid: 11005837
references.citation: Nat Mater. 2003 Mar;2(3):196-200
references.pmid: 12612679
references.citation: Biotechnol Prog. 2006 Mar-Apr;22(2):401-5
references.pmid: 16599553
references.citation: Science. 2006 Nov 10;314(5801):1001-4
references.pmid: 17095702
references.citation: Proc Natl Acad Sci U S A. 1998 Oct 13;95(21):12283-8
references.pmid: 9770478
references.citation: Proc Natl Acad Sci U S A. 2002 Apr 30;99(9):5977-81
references.pmid: 11959922
references.citation: Phys Rev Lett. 2006 Mar 17;96(10):108101
references.pmid: 16605793
references.citation: Eur Biophys J. 2008 Jul;37(6):927-34
references.pmid: 18379774
references.citation: Proc Natl Acad Sci U S A. 1999 Mar 30;96(7):3694-9
references.pmid: 10097099
references.citation: Proc Natl Acad Sci U S A. 2010 Jan 5;107(1):5-10
references.pmid: 20080797
references.citation: Proc Natl Acad Sci U S A. 2006 Aug 29;103(35):12999-3003
references.pmid: 16920796