title: Anti-inflammatory study on crude methanol extract and different fractions of Eremostachys laciniata.
abstract: BACKGROUND
  Eremostachys laciniata (L.) Bunge (Lamiaceae), which has been reported as a rich source of flavonoids, is one of the rarely explored species of the genus Eremostachys.
  
  
  OBJECTIVE
  In this study, the crude methanol extract and different fractions of E. laciniata were investigated for in vivo anti-inflammatory properties.
  
  
  METHODS
  Shade-dried leaves of E. laciniata were exhaustively extracted by percolation with methanol (80%) to obtain 250 g of crude methanol extract (El), followed by fractionation with different organic solvents to get the n-hexane (Elh), chloroform (Elc), ethyl acetate (Ele), butanol (Elb), and water (Elw) fractions. An in vivo anti-inflammatory study of the crude extract and sub-crude fractions was carried out in rats using the carrageenan model.
  
  
  RESULTS
  The Ele fraction was found to be the most potent inhibitor of edema formation by inducing a maximum inhibitory effect of 74.2% at the 300 mg/kg dose, during 3 h post carrageenan injection. The El extract and Elc fraction also showed good anti-inflammatory properties at the same dose.
  
  
  CONCLUSIONS
  The demonstration of excellent anti-inflammatory activity by the plant chiefly concentrating in the Ele fraction and the appearance of peak activity in the latter phase of the experiment suggested the presence of relatively low-polar substances with arachidonic acid metabolite inhibition property.
  
  
  CONCLUSIONS
  The plant may be an excellent source in the future for activity-guided isolation of important anti-inflammatory substances.
authors.affiliation: Department of Pharmacy, University of Peshawar, Peshawar, Pakistan. salim41us@yahoo.com
authors.forename: Salimullah
authors.initials: S
authors.lastname: Khan
authors.forename: Muhammad
authors.initials: M
authors.lastname: Nisar
authors.forename: Wajid
authors.initials: W
authors.lastname: Rehman
authors.forename: Razaullah
authors.initials: R
authors.lastname: Khan
authors.forename: Fazli
authors.initials: F
authors.lastname: Nasir
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology / Q000627:therapeutic use*; D002351:Carrageenan; D005591:Chemical Fractionation; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy*; D005260:Female; D019686:Lamiaceae / Q000737:chemistry*; D008297:Male; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D018515:Plant Leaves / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000893:Anti-Inflammatory Agents; D010936:Plant Extracts; D002351:Carrageenan