Oocyte cohesin expression restricted to predictyate stages provides full fertility and prevents aneuploidy.
To ensure correct meiotic chromosome segregation, sister chromatid cohesion (SCC) needs to be maintained from its establishment in prophase I oocytes before birth until continuation of meiosis into metaphase II upon oocyte maturation in the adult. Aging human oocytes suffer a steep increase in chromosome missegregation and aneuploidy, which may be caused by loss of SCC through slow deterioration of cohesin [1-3]. This hypothesis assumes that cohesin expression in embryonic oocytes is sufficient to provide adequate long-term SCC. With increasing age, mouse oocytes deficient in the meiosis-specific cohesin SMC1β massively lose SCC and chiasmata [3, 4]. To test the deterioration hypothesis, we specifically and highly efficiently inactivated the mouse Smc1β gene at the primordial follicle stage shortly after birth, when oocytes had just entered meiosis I dictyate arrest. In the adult, however, irrespective of oocyte age, chiasma positions and SCC are normal. Frequency and size of litters prove full fertility even in aged females. Thus, SMC1β cohesin needs only be expressed during prophase I prior to the primordial follicle stage to ensure SCC up to advanced age of mice.
Revenkova
Ekaterina
E
Department of Developmental and Regenerative Biology, Mount Sinai School of Medicine, One Gustave L. Levy Place, New York, NY 10029-6574, USA. ekaterina.revenkova@mssm.edu
Herrmann
Kathleen
K
Adelfalk
Caroline
C
Jessberger
Rolf
R
D000782:Aneuploidy; D000818:Animals; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics / Q000378:metabolism*; D005260:Female; D005298:Fertility / Q000235:genetics*; D008297:Male; D008540:Meiosis; D051379:Mice; D009865:Oocytes / Q000166:cytology / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000097722:Cohesins
D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; D000097722:Cohesins; C438001:Smc1l2 protein, mouse
Nat Genet. 2000 Jun;25(2):139-40
10835623
Methods Mol Biol. 2000;132:365-86
10547847
Cell. 2005 Nov 4;123(3):371-3
16269329
Trends Genet. 2008 Feb;24(2):86-93
18192063
Hum Reprod Update. 2008 Mar-Apr;14(2):143-58
18084010
Annu Rev Genet. 2009;43:525-58
19886810
Hum Reprod. 2010 Sep;25(9):2316-27
20634189
Nat Genet. 2005 Dec;37(12):1303-4
16314861
J Cell Sci. 2000 Feb;113 ( Pt 4):673-82
10652260
Nat Rev Genet. 2001 Apr;2(4):280-91
11283700
Nat Genet. 2005 Dec;37(12):1351-5
16258540
Biol Reprod. 2004 Nov;71(5):1469-74
15215191
Dev Biol. 2006 Oct 1;298(1):149-54
16925987
Nat Rev Genet. 2010 Jun;11(6):391-404
20442714
J Cell Biol. 2009 Oct 19;187(2):185-99
19841137
Reprod Biomed Online. 2008 Jan;16(1):103-12
18252055
Nat Rev Genet. 2010 Feb;11(2):124-36
20051984
Mol Cell Biol. 2001 Oct;21(20):6984-98
11564881
Nat Cell Biol. 2004 Jun;6(6):555-62
15146193