Metabolic engineering of Escherichia coli for the production of cadaverine: a five carbon diamine.
A five carbon linear chain diamine, cadaverine (1,5-diaminopentane), is an important platform chemical having many applications in chemical industry. Bio-based production of cadaverine from renewable feedstock is a promising and sustainable alternative to the petroleum-based chemical synthesis. Here, we report development of a metabolically engineered strain of Escherichia coli that overproduces cadaverine in glucose mineral salts medium. First, cadaverine degradation and utilization pathways were inactivated. Next, L-lysine decarboxylase, which converts L-lysine directly to cadaverine, was amplified by plasmid-based overexpression of the cadA gene under the strong tac promoter. Furthermore, the L-lysine biosynthetic pool was increased by the overexpression of the dapA gene encoding dihydrodipicolinate synthase through the replacement of the native promoter with the strong trc promoter in the genome. The final engineered strain was able to produce 9.61 g L(-1) of cadaverine with a productivity of 0.32 g L(-1) h(-1) by fed-batch cultivation. The strategy reported here should be useful for the bio-based production of cadaverine from renewable resources.
Qian
Zhi-Gang
ZG
Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical & Biomolecular Engineering (BK21 Program), BioProcess Engineering Research Center, Center for Systems and Synthetic Biotechnology, and Institute for BioCentury, KAIST, 335 Gwahangno, Yuseong-gu, Daejeon 305-701, Republic of Korea.
Xia
Xiao-Xia
XX
Lee
Sang Yup
SY
D053898:Biosynthetic Pathways / Q000235:genetics*; D001709:Biotechnology / Q000379:methods*; D002103:Cadaverine / Q000096:biosynthesis*; D002262:Carboxy-Lyases / Q000235:genetics; D003001:Cloning, Molecular; D003470:Culture Media / Q000737:chemistry; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D005285:Fermentation; D017353:Gene Deletion; D015870:Gene Expression; D005818:Genetic Engineering; D005947:Glucose / Q000378:metabolism; D006836:Hydro-Lyases / Q000096:biosynthesis; D008239:Lysine / Q000096:biosynthesis; D010957:Plasmids; D011401:Promoter Regions, Genetic
D003470:Culture Media; D002262:Carboxy-Lyases; C021520:lysine decarboxylase; D006836:Hydro-Lyases; C020040:4-hydroxy-tetrahydrodipicolinate synthase; D005947:Glucose; D008239:Lysine; D002103:Cadaverine