title: Modulation of viability of live cells by focused ion-beam exposure.
abstract: Introduction of membrane-impermeant substances into living cells is the key method to understand contemporary cellular processes by investigating cellular responses and phenotypes. Here, we performed gold ion beam exposure into live cells by using the focused ion beam implantation method, which was originally developed to precisely control semiconductor device performances. We evaluated the viability of the gold-irradiated cells by measuring the concentration of adenosine triphosphate (ATP), which is an intracellular energy source produced in the mitochondrial membrane. The viability of the irradiated cells was found to be 20% higher than that of the unirradiated control cells. The atoms might promote the energy generating processes within the mitochondrion. Our results suggest that the viability of living cells can be modulated by accurately controlling the dopant atom numbers. Our technique may be considered as a potential tool in life and medical sciences to quantitatively elucidate the dose-dependent effects of dopants.
authors.affiliation: Waseda Institute for Advanced Study, Waseda University, 1-6-1 Nishiwaseda, Shinjuku, Tokyo 169-8050, Japan. shinada@aoni.waseda.jp
authors.forename: Takahiro
authors.initials: T
authors.lastname: Shinada
authors.forename: Takayuki
authors.initials: T
authors.lastname: Akimoto
authors.forename: Yanwei
authors.initials: Y
authors.lastname: Zhu
authors.forename: Hisa
authors.initials: H
authors.lastname: Goke
authors.forename: Iwao
authors.initials: I
authors.lastname: Ohdomari
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis; D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D006046:Gold / Q000378:metabolism*; D007477:Ions / Q000378:metabolism*; D051379:Mice; D032446:Myoblasts / Q000737:chemistry / Q000187:drug effects* / Q000502:physiology*
chemical_list: D007477:Ions; D006046:Gold; D000255:Adenosine Triphosphate