Site-specific incorporation of perylene into an N-terminally modified light-harvesting complex II.
Employing the utility of the native chemical ligation, site-specific attachment of an ultrastable perylene dye to a derivative of the major light-harvesting complex (LHCII) was demonstrated. Biochemical analysis of the conjugate indicated that the structure and function of LHCII remain largely unaffected by the N-terminal modification.
Peneva
Kalina
K
Max Planck Institute for Polymer Research, Ackermannweg 10, 55128, Mainz, Germany.
Gundlach
Kristina
K
Herrmann
Andreas
A
Paulsen
Harald
H
Müllen
Klaus
K
D005456:Fluorescent Dyes / Q000737:chemistry*; D045342:Light-Harvesting Protein Complexes / Q000737:chemistry* / Q000378:metabolism; D008969:Molecular Sequence Data; D010569:Perylene / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry* / Q000378:metabolism; D010944:Plants / Q000737:chemistry / Q000378:metabolism
D005456:Fluorescent Dyes; D045342:Light-Harvesting Protein Complexes; D010940:Plant Proteins; D010569:Perylene