title: Expression, purification and crystallization of Chaetomium thermophilum Cu,Zn superoxide dismutase.
abstract: Cu,Zn superoxide dismutase (Cu,ZnSOD) from the thermophilic fungus Chaetomium thermophilum was expressed in Pichia pastoris and purified. Crystals were grown in over 120 conditions but only those produced with 1.4 M sodium potassium phosphate pH 8.2 as precipitant were suitable for structural studies. Data were collected to 1.9 A resolution at 100 K from a single crystal using a synchrotron-radiation source. The crystals belonged to space group P6(1)/P6(5), with unit-cell parameters a=90.2, c=314.5 A and eight molecules in the asymmetric unit. Elucidation of the crystal structure will provide insights into the active site of the enzyme and a better understanding of the structure-activity relationship, assembly and thermal stability of Cu,ZnSODs.
authors.affiliation: Turku Centre for Biotechnology, University of Turku and Abo Akademi, Turku 20521, Finland.
authors.forename: Sachin
authors.initials: S
authors.lastname: Wakadkar
authors.forename: Li-Qing
authors.initials: LQ
authors.lastname: Zhang
authors.forename: Duo-Chuan
authors.initials: DC
authors.lastname: Li
authors.forename: Teemu
authors.initials: T
authors.lastname: Haikarainen
authors.forename: Prathusha
authors.initials: P
authors.lastname: Dhavala
authors.forename: Anastassios C
authors.initials: AC
authors.lastname: Papageorgiou
mesh_terms: D002597:Chaetomium / Q000201:enzymology*; D003460:Crystallization; D018360:Crystallography, X-Ray; D015870:Gene Expression; D013482:Superoxide Dismutase / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D013482:Superoxide Dismutase
references.citation: Biochim Biophys Acta. 2010 Feb;1804(2):245-62
references.pmid: 19914407
references.citation: Biochim Biophys Acta. 2010 Feb;1804(2):263-74
references.pmid: 19914406
references.citation: Anal Biochem. 1976 May 7;72:248-54
references.pmid: 942051
references.citation: CRC Crit Rev Biochem. 1987;22(2):111-80
references.pmid: 3315461
references.citation: Microbiol Mol Biol Rev. 2000 Sep;64(3):461-88
references.pmid: 10974122
references.citation: Plant Physiol. 1980 Feb;65(2):245-8
references.pmid: 16661168
references.citation: Int J Biochem Cell Biol. 2000 Feb;32(2):157-70
references.pmid: 10687951
references.citation: J Mol Biol. 2003 Sep 19;332(3):601-15
references.pmid: 12963370
references.citation: Acta Crystallogr D Biol Crystallogr. 2010 Feb;66(Pt 2):125-32
references.pmid: 20124692
references.citation: J Aerosol Med Pulm Drug Deliv. 2008 Sep;21(3):281-90
references.pmid: 18578594
references.citation: Annu Rev Biochem. 2008;77:755-76
references.pmid: 18173371
references.citation: J Mol Biol. 2009 Feb 6;385(5):1534-55
references.pmid: 19063897
references.citation: Nat Med. 1998 Jun;4(6):698-704
references.pmid: 9623979
references.citation: Curr Opin Oncol. 1997 Nov;9(6):569-78
references.pmid: 9370079
references.citation: Adv Genet. 1989;26:65-97
references.pmid: 2554697
references.citation: Nat Struct Biol. 1997 Apr;4(4):269-75
references.pmid: 9095194
references.citation: Microbiology (Reading). 2001 Jun;147(Pt 6):1641-1650
references.pmid: 11390695
references.citation: Biosci Biotechnol Biochem. 2007 Apr;71(4):1090-3
references.pmid: 17420576
references.citation: J Mol Biol. 1968 Apr 28;33(2):491-7
references.pmid: 5700707
references.citation: J Biol Chem. 2004 Mar 5;279(10):9248-54
references.pmid: 14672935
references.citation: Annu Rev Biochem. 1995;64:97-112
references.pmid: 7574505