title: Detection of metallo-β-lactamase genes in clinical specimens by a commercial multiplex PCR system.
abstract: hyplex®-MBL ID Multiplex PCR-ELISA, a novel method for identifying metallo-β-lactamase genes directly in clinical specimens, was evaluated using a consecutive collection of 326 samples from three hospitals in Greece characterized by high prevalence of VIM producers. The method exhibited high sensitivity (98.0%) and specificity (98.6%) and was proven reliable in detecting bla(VIM) genes in blood, urine, pus, and sputum samples that, as confirmed by conventional methods, contained various VIM-producing species. Future multicenter studies should be considered for the thorough evaluation of this method and its potential diagnostic utility.
authors.affiliation: Department of Microbiology, Laikon General Hospital, Athens, Greece.
authors.forename: Athina
authors.initials: A
authors.lastname: Avlami
authors.forename: S
authors.initials: S
authors.lastname: Bekris
authors.forename: G
authors.initials: G
authors.lastname: Ganteris
authors.forename: Eleni
authors.initials: E
authors.lastname: Kraniotaki
authors.forename: Eleni
authors.initials: E
authors.lastname: Malamou-Lada
authors.forename: Maria
authors.initials: M
authors.lastname: Orfanidou
authors.forename: Olga
authors.initials: O
authors.lastname: Paniara
authors.forename: Angeliki
authors.initials: A
authors.lastname: Pantazatou
authors.forename: C C
authors.initials: CC
authors.lastname: Papagiannitsis
authors.forename: Evangelia
authors.initials: E
authors.lastname: Platsouka
authors.forename: Ioanna
authors.initials: I
authors.lastname: Stefanou
authors.forename: Eva
authors.initials: E
authors.lastname: Tzelepi
authors.forename: Helen
authors.initials: H
authors.lastname: Vagiakou
authors.forename: Vivi
authors.initials: V
authors.lastname: Miriagou
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006090:Gram-Negative Bacteria / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology*; D006115:Greece; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D001618:beta-Lactamases / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D001618:beta-Lactamases