Torque-induced slip of the rotary motor F1-ATPase.
F(1)-ATPase plays an essential role in cellular metabolism by linking rotational motion to ATP hydrolysis/synthesis. We measure the torque profile of F(1) in both ATP hydrolysis and synthesis directions using a novel magnetic nanorod assay. F(1) is found to decouple ATP synthesis from rotary motion at a surprisingly low torque. This low-torque slip mechanism protects the enzyme from excessive load and may play a broader biological role by reducing the production of reactive oxygen species.
Palanisami
Akilan
A
Department of Physics, Faculty of Science and Engineering, Waseda University, Okubo 3-4-1, Shinjuku-ku, Tokyo 169-8555, Japan. apalanisami@uh.edu
Okamoto
Tetsuaki
T
D000255:Adenosine Triphosphate / Q000378:metabolism*; D001671:Biochemistry / Q000379:methods; D006868:Hydrolysis; D008280:Magnetics / Q000379:methods*; D043942:Nanotubes / Q000737:chemistry*; D006180:Proton-Translocating ATPases / Q000737:chemistry / Q000378:metabolism*; D019415:Torque
D000255:Adenosine Triphosphate; D006180:Proton-Translocating ATPases