title: [Monoclonal antibodies against human secretory component: epitope specificity and utility for immunoanalysis].
abstract: OBJECTIVE
  To develop and characterize by immunochemical methods the panel of monoclonal antibodies (MAbs) recognizing different antigenic determinants ofhuman secretory component (SC) molecule.
  
  
  METHODS
  sIgA and SC were obtained from colostrum by combination of ion-exchange chromatography and gel filtration. Recombinant SC was expressed in Escherichia coli cells transformed by construction that contained fragment of gene coding extracellular domain of receptor for polymeric Ig. MAbs were produced and studied using hybridoma technologies and different methods of immunoenzyme analysis respectively.
  
  
  RESULTS
  Panel comprising 10 MAbs against human SC of which 4 types of antibodies recognize cryptic epitopes of free SC and other 6 types recognize epitopes exposed both on SC and sIgA. MAbs panel contains antibodies interacting with conformational and linear epitopes of antigen. Three from obtained MAbs bind to SC epitopes which structure is determined by presence of carbohydrate residues in the molecule of antigen. Immunometric systems were developed which allow to differentially detect free SC and sIgA.
  
  
  CONCLUSIONS
  Developed and characterized MAbs panel recognizing different epitopes of SC molecule opens new opportunities for laboratory and basic research of human secretory immunity.
authors.forename: I V
authors.initials: IV
authors.lastname: Griazeva
authors.forename: M P
authors.initials: MP
authors.lastname: Samoĭlovich
authors.forename: B V
authors.initials: BV
authors.lastname: Klimovich
authors.forename: M S
authors.initials: MS
authors.lastname: Pavlova
authors.forename: N L
authors.initials: NL
authors.lastname: Vartanian
authors.forename: A N
authors.initials: AN
authors.lastname: Kirienko
authors.forename: V B
authors.initials: VB
authors.lastname: Klimovich
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D003126:Colostrum / Q000276:immunology*; D000939:Epitopes / Q000276:immunology*; D006801:Humans; D007071:Immunoglobulin A, Secretory / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011994:Recombinant Proteins / Q000276:immunology; D012635:Secretory Component / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D007071:Immunoglobulin A, Secretory; D011994:Recombinant Proteins; D012635:Secretory Component