Hydrothermal synthesis of α-Fe(2)O(3)@SnO(2) core-shell nanotubes for highly selective enrichment of phosphopeptides for mass spectrometry analysis.
In this work, we synthesized α-Fe(2)O(3)@SnO(2) core-shell structure nanotubes by a facile two-step hydrothermal method for selective enrichment of phosphopeptides. The core-shell structure nanotubes were found to have outer diameters of 90-110 nm, thicknesses of 30 nm, and lengths of 260-280 nm. SnO(2) nanoparticles with an average crystallite size of around 5 nm form a layer of about 10 nm on both walls of the α-Fe(2)O(3) nanotubes. To demonstrate their ability for selective enrichment of phosphopeptides, we applied α-Fe(2)O(3)@SnO(2) nanotubes to the isolation and enrichment of the phosphopeptides from standard protein digestion and real samples. The enriched peptides were analyzed by MALDI-MS and LC-ESI MS. Experiment results demonstrate that SnO(2) coated α-Fe(2)O(3) nanotubes show excellent potential for selective enrichment of phosphopeptides.
Lu
Jin
J
Department of Chemistry & Institutes of Biomedical Sciences, Fudan University, Shanghai 200433, China.
Qi
Dawei
D
Deng
Chunhui
C
Zhang
Xiangmin
X
Yang
Pengyuan
P
D000818:Animals; D002417:Cattle; D002853:Chromatography, Liquid / Q000379:methods; D000431:Ethanol / Q000737:chemistry; D005290:Ferric Compounds / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods*; D036103:Nanotechnology / Q000379:methods; D043942:Nanotubes / Q000737:chemistry; D010455:Peptides / Q000737:chemistry; D010748:Phosphopeptides / Q000737:chemistry*; D051381:Rats; D012709:Serum Albumin / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D017971:Tin Compounds / Q000737:chemistry*; D014357:Trypsin / Q000737:chemistry
D005290:Ferric Compounds; D010455:Peptides; D010748:Phosphopeptides; D012709:Serum Albumin; D017971:Tin Compounds; C000499:ferric oxide; D000431:Ethanol; D014357:Trypsin; C045358:stannic oxide