title: Computational Identification of Novel MicroRNAs and Their Targets in Vigna unguiculata.
abstract: MicroRNAs (miRNAs) are a class of endogenous, noncoding, short RNAs directly involved in regulating gene expression at the posttranscriptional level. High conservation of miRNAs in plant provides the foundation for identification of new miRNAs in other plant species through homology alignment. Here, previous known plant miRNAs were BLASTed against the Expressed Sequence Tag (EST) and Genomic Survey Sequence (GSS) databases of Vigna unguiculata, and according to a series of filtering criteria, a total of 47 miRNAs belonging to 13 miRNA families were identified, and 30 potential target genes of them were subsequently predicted, most of which seemed to encode transcription factors or enzymes participating in regulation of development, growth, metabolism, and other physiological processes. Overall, our findings lay the foundation for further researches of miRNAs function in Vigna unguiculata.
authors.affiliation: Biology Department, Qingdao University of Science and Technology, Qingdao 266043, China.
authors.forename: Yongzhong
authors.initials: Y
authors.lastname: Lu
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Yang
references.citation: Plant Cell. 2005 Aug;17(8):2186-203
references.pmid: 15994906
references.citation: FASEB J. 2001 May;15(7):1118-31
references.pmid: 11344080
references.citation: Comput Biol Chem. 2009 Feb;33(1):84-93
references.pmid: 18723398
references.citation: Planta. 2009 Sep;230(4):671-85
references.pmid: 19585144
references.citation: Cell. 2009 Aug 21;138(4):625-7
references.pmid: 19703391
references.citation: Proc Natl Acad Sci U S A. 2009 Oct 27;106(43):18159-64
references.pmid: 19826088
references.citation: Annu Rev Plant Biol. 2009;60:485-510
references.pmid: 19519217
references.citation: Cell Res. 2005 May;15(5):336-60
references.pmid: 15916721
references.citation: Development. 2007 May;134(9):1635-41
references.pmid: 17409118
references.citation: Int J Plant Genomics. 2009;2009:262463
references.pmid: 19834623
references.citation: PLoS Biol. 2009 Jul;7(7):e1000148
references.pmid: 19582143
references.citation: Proc Natl Acad Sci U S A. 2009 Dec 29;106(52):22534-9
references.pmid: 20007771
references.citation: Development. 2010 Jan;137(1):103-12
references.pmid: 20023165
references.citation: Cell Mol Life Sci. 2006 Jan;63(2):246-54
references.pmid: 16395542
references.citation: J Biomed Biotechnol. 2009;2009:803069
references.pmid: 19551154
references.citation: Science. 2004 Mar 26;303(5666):2022-5
references.pmid: 12893888
references.citation: Curr Opin Plant Biol. 2007 Oct;10(5):453-60
references.pmid: 17900969
references.citation: Bioorg Chem. 2004 Oct;32(5):316-25
references.pmid: 15381398
references.citation: Sci China C Life Sci. 2009 Nov;52(11):1091-100
references.pmid: 19937208
references.citation: Gene. 2008 May 15;414(1-2):60-6
references.pmid: 18387754
references.citation: BMC Plant Biol. 2008 Apr 16;8:37
references.pmid: 18416839
references.citation: Methods Mol Biol. 2010;592:51-7
references.pmid: 19802588
references.citation: Arch Biochem Biophys. 1999 Apr 15;364(2):273-9
references.pmid: 10190984
references.citation: Sex Plant Reprod. 2009 Dec;22(4):277-89
references.pmid: 20033449
references.citation: Plant Physiol. 2008 Jan;146(1):97-107
references.pmid: 17981987
references.citation: BMC Microbiol. 2010 Mar 23;10:89
references.pmid: 20331875
references.citation: Biochem Biophys Res Commun. 2009 Jan 23;378(4):799-803
references.pmid: 19084500
references.citation: Methods Mol Biol. 2010;592:149-61
references.pmid: 19802595
references.citation: FEBS Lett. 2007 Apr 3;581(7):1464-74
references.pmid: 17367786
references.citation: BMC Genomics. 2008 May 28;9:248
references.pmid: 18507836
references.citation: Development. 2006 Sep;133(18):3539-47
references.pmid: 16914499
references.citation: Proc Natl Acad Sci U S A. 2004 Aug 3;101(31):11511-6
references.pmid: 15272084
references.citation: Nucleic Acids Res. 2003 Jul 1;31(13):3406-15
references.pmid: 12824337
references.citation: EMBO J. 2009 Jul 22;28(14):2090-9
references.pmid: 19536137
references.citation: Plant J. 2006 Apr;46(2):243-59
references.pmid: 16623887
references.citation: Trends Plant Sci. 2010 Jan;15(1):31-9
references.pmid: 19963426
references.citation: Biochem Soc Trans. 2002 Aug;30(4):584-90
references.pmid: 12196142
references.citation: Trends Plant Sci. 2007 Jul;12(7):301-9
references.pmid: 17573231
references.citation: EMBO J. 2004 Oct 13;23(20):4051-60
references.pmid: 15372072
references.citation: Science. 2003 Jul 18;301(5631):336-8
references.pmid: 12869753
references.citation: Oncogene. 2010 Apr 15;29(15):2161-4
references.pmid: 20190803
references.citation: Cell. 2009 Aug 21;138(4):738-49
references.pmid: 19703399
references.citation: Biochem Biophys Res Commun. 2009 Aug 14;386(1):6-10
references.pmid: 19501049
references.citation: RNA. 2003 Mar;9(3):277-9
references.pmid: 12592000
references.citation: Cell. 2004 Jan 23;116(2):281-97
references.pmid: 14744438