Hypermethylation of SOX2 Promoter in Endometrial Carcinogenesis.
This paper aimed at investigating the expression and methylation profiles of SOX2, a gene coding for the stem cell-related transcription factor SOX2, in endometrial carcinomas. By methylation-specific polymerase chain reaction (MS-PCR), the methylation status of SOX2 promoter region in 72 endometrial carcinomas and 12 normal endometrial samples was examined. Methylated allele was found in 37.5% (27/72) of endometrial carcinomas but only in 8.3% (1/12) of normal endometrial, significantly more frequent in cancers (P = .0472). SOX2 mRNA level was significantly reduced in endometrial carcinoma compared with nonneoplastic endometrium (P = .045). A significant correlation between SOX2 mRNA expression and hypermethylation of SOX2 was found (P = .024). Hypermethylation of SOX2 tended to be more frequently found in type II serous or clear cell adenocarcinoma. SOX2 methylation was also significantly correlated with shorter survival of patients (P = .046). In conclusion, epigenetic mechanisms may play a crucial role on the transcriptional regulation of SOX2 and loss of SOX2 expression may be related to endometrial carcinogenesis.
Wong
Oscar Gee-Wan
OG
Department of Pathology, Queen Mary Hospital, The University of Hong Kong, Pokfulam Road, Hong Kong.
Huo
Zhen
Z
Siu
Michelle Kwan-Yee
MK
Zhang
Huijuan
H
Jiang
Lili
L
Wong
Ester Shuk-Ying
ES
Cheung
Annie Nga-Yin
AN
Mod Pathol. 2007 Apr;20(4):474-81
17334350
BMC Cancer. 2006 Aug 23;6:212
16928264
Br J Cancer. 2007 Apr 23;96(8):1293-301
17375044
J Mol Diagn. 2004 Nov;6(4):326-34
15507671
Biochem Biophys Res Commun. 2009 May 29;383(2):157-62
19268426
Br J Cancer. 2008 Feb 26;98(4):824-31
18268498
J Biol Chem. 2008 Jun 27;283(26):17969-78
18456656
Gynecol Oncol. 2009 Mar;112(3):537-42
19108875
Mod Pathol. 2007 Oct;20(10):1045-54
17673926
Gynecol Oncol. 1998 Jun;69(3):220-5
9648591
Oncol Res. 2006;16(4):205-9
17120618
Science. 2000 Sep 8;289(5485):1751-4
10976068
Dev Biol. 2000 Nov 15;227(2):239-55
11071752
Gynecol Oncol. 2000 Apr;77(1):55-65
10739691
J Cancer Res Clin Oncol. 2004 Mar;130(3):135-45
14655050
Am J Physiol Gastrointest Liver Physiol. 2009 Aug;297(2):G312-22
19520737
Cell. 2006 Aug 25;126(4):663-76
16904174
Cancer. 2001 Mar 1;91(5):1052-60
11251959
Stem Cells. 2009 Jan;27(1):40-8
18948646
Tumour Biol. 2009;30(2):93-9
19420986
Stem Cells. 2008 Aug;26(8):1931-8
18388306
Blood. 2009 May 28;113(22):5476-9
19299331
Dev Cell. 2002 Aug;3(2):167-70
12194848
Trends Neurosci. 2005 Nov;28(11):583-8
16139372
Virchows Arch. 2004 Mar;444(3):213-23
14747944
Cancer Epidemiol Biomarkers Prev. 2008 Mar;17(3):571-7
18349274
EMBO J. 1990 May;9(5):1603-14
2328727
Reprod Sci. 2008 Sep;15(7):735-44
18836133
Proc Natl Acad Sci U S A. 2000 Apr 11;97(8):4198-203
10760287
Int J Cancer. 2008 Jul 15;123(2):296-302
18404674
Maturitas. 2009 Apr 20;62(4):334-7
19231117
Anticancer Res. 2009 Apr;29(4):1233-41
19414369
Nat Rev Cancer. 2006 May;6(5):360-8
16633364
CA Cancer J Clin. 2007 Jan-Feb;57(1):43-66
17237035
Lancet. 2005 Aug 6-12;366(9484):491-505
16084259