title: AtCML8, a calmodulin-like protein, differentially activating CaM-dependent enzymes in Arabidopsis thaliana.
abstract: Plants express many calmodulins (CaMs) and calmodulin-like (CML) proteins that sense and transduce different Ca(2+) signals. Previously, we reported divergent soybean (Glycine max) CaM isoforms (GmCaM4/5) with differential abilities to activate CaM-dependent enzymes. To elucidate biological functions of divergent CaM proteins, we isolated a cDNA encoding a CML protein, AtCML8, from Arabidopsis. AtCML8 shows highest identity with GmCaM4 at the protein sequence level. Expression of AtCML8 was high in roots, leaves, and flowers but low in stems. In addition, the expression of AtCML8 was induced by exposure to salicylic acid or NaCl. AtCML8 showed typical characteristics of CaM such as Ca(2+)-dependent electrophoretic mobility shift and Ca(2+) binding ability. In immunoblot analyses, AtCML8 was recognized only by antiserum against GmCaM4 but not by GmCaM1 antibodies. Interestingly, AtCML8 was able to activate phosphodiesterase (PDE) but did not activate NAD kinase. These results suggest that AtCML8 acts as a CML protein in Arabidopsis with characteristics similar to soybean divergent GmCaM4 at the biochemical levels.
authors.affiliation: Division of Applied Life Science (BK21 Program), Plant Molecular Biology and Biotechnology Research Center, Jinju, Korea. hcpark@gnu.ac.kr
authors.forename: Hyeong Cheol
authors.initials: HC
authors.lastname: Park
authors.forename: Chan Young
authors.initials: CY
authors.lastname: Park
authors.forename: Sung Cheol
authors.initials: SC
authors.lastname: Koo
authors.forename: Mi Sun
authors.initials: MS
authors.lastname: Cheong
authors.forename: Kyung Eun
authors.initials: KE
authors.lastname: Kim
authors.forename: Min Chul
authors.initials: MC
authors.lastname: Kim
authors.forename: Chae Oh
authors.initials: CO
authors.lastname: Lim
authors.forename: Sang Yeol
authors.initials: SY
authors.lastname: Lee
authors.forename: Dae-Jin
authors.initials: DJ
authors.lastname: Yun
authors.forename: Woo Sik
authors.initials: WS
authors.lastname: Chung
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002147:Calmodulin / Q000235:genetics / Q000378:metabolism*; D018076:DNA, Complementary / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D010802:Phylogeny; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D020156:Salicylic Acid / Q000494:pharmacology; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000494:pharmacology
chemical_list: D029681:Arabidopsis Proteins; D002147:Calmodulin; D018076:DNA, Complementary; D018744:DNA, Plant; D020033:Protein Isoforms; D012965:Sodium Chloride; D020156:Salicylic Acid
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