title: Comparison of polymerase chain reaction tests for diagnosis of feline herpesvirus, Chlamydophila felis, and Mycoplasma spp. infection in cats with ocular disease in Canada.
abstract: This study assessed the value of polymerase chain reaction (PCR) for making a diagnosis of feline herpesvirus (FHV-1) infection, and for differentiating this from Chlamydophila felis and Mycoplasma spp. infection in a clinical setting in Canada. We compared the frequency of positive FHV-1 PCR test results from 48 clinical cases of ocular disease in cats suspected to be due to FHV-1 between 1 research and 2 commercial laboratories in Canada. We also compared PCR results for Chlamydophila felis and Mycoplasma spp. between the 2 commercial laboratories. The prevalence of FHV-1 infection in the cats ranged from 4% to 21%. The prevalence of Chlamydophila felis was 2% and 17% and the prevalence of Mycoplasma spp. was 11% and 27%. Agreement between FHV-1 culture and PCR results at the research laboratory was substantial (kappa = 0.76). There was slight agreement (kappa < 0.20) between the 3 laboratories for FHV-1 PCR and between the 2 commercial laboratories for both Chlamydophila felis (kappa = 0.2) and Mycoplasma spp. (kappa = 0.07) PCR.
authors.affiliation: Departments of Small Animal, University of Saskatchewan, Saskatoon, Canada. lss114@mail.usask.ca
authors.forename: Lynne S
authors.initials: LS
authors.lastname: Sandmeyer
authors.forename: Cheryl L
authors.initials: CL
authors.lastname: Waldner
authors.forename: Bianca S
authors.initials: BS
authors.lastname: Bauer
authors.forename: Xin
authors.initials: X
authors.lastname: Wen
authors.forename: Dorothee
authors.initials: D
authors.lastname: Bienzle
mesh_terms: D000818:Animals; D002170:Canada; D002371:Cat Diseases / Q000175:diagnosis* / Q000382:microbiology / Q000821:virology; D002415:Cats; D023501:Chlamydophila / Q000302:isolation & purification; D023521:Chlamydophila Infections / Q000175:diagnosis / Q000662:veterinary; D003937:Diagnosis, Differential; D015817:Eye Infections / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary* / Q000821:virology; D005260:Female; D006564:Herpesviridae / Q000302:isolation & purification; D006566:Herpesviridae Infections / Q000175:diagnosis / Q000662:veterinary; D008297:Male; D009174:Mycoplasma / Q000302:isolation & purification; D009175:Mycoplasma Infections / Q000175:diagnosis / Q000662:veterinary; D016133:Polymerase Chain Reaction / Q000662:veterinary*
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