Cadmium toxicity on the freshwater microalga Chlamydomonas moewusii Gerloff: Biosynthesis of thiol compounds.
Cadmium (Cd) toxicity and production of different thiols (phytochelatins, glutathione, gamma-Glu-Cys and cysteine) were studied in the microalga Chlamydomonas moewusii exposed to different concentrations of this metal (1, 2, 4, 6, 8, and 10 mg/L) for 96 h. The inhibitory effect of Cd on growth was demonstrated. The value of EC50 (metal concentration which reduces the population growth to 50% of the control) obtained for this microalga was estimated at 4.1 +/- 0.8 mg/L of Cd after 96 h of exposure. The amount of thiol compounds synthesized by C. moewusii changed with Cd concentration. Cysteine concentrations were significantly higher compared to those of gamma-Glu-Cys and glutathione in all the Cd concentrations assayed. The amino acid cysteine reached its higher levels in those cultures in which a decrease in the concentration of phytochelatins (PCs) was observed. Both cysteine and glutathione concentrations showed significant differences along the Cd concentrations assayed, while the amount of gamma-Glu-Cys detected remained stable. The PCs detected were of two, three, and four subunits. The level of PC(2) was higher than that of PC(3) and PC(4). PC(4) was detected only in the cultures exposed to the Cd concentrations of 1 and 2 mg/L, in which the synthesis of phytochelatins was higher. A rapid increase in the production of PC(2) and PC(3) was observed up to a Cd concentration of 2 mg/L, after which their levels began to decrease. Phytochelatins were not detected in cultures without Cd (controls) and in those exposed to the maximum Cd concentration (10 mg/L), in which cell growth was completely inhibited.
Suárez
Cristina
C
Laboratorio de Microbiología, Facultad de Ciencias, Universidade da Coruña, Alejandro de La Sota, No. 1. 15008. La Coruña, Spain.
Torres
Enrique
E
Pérez-Rama
Mónica
M
Herrero
Concepción
C
Abalde
Julio
J
D053898:Biosynthetic Pathways / Q000187:drug effects; D002104:Cadmium / Q000633:toxicity*; D002696:Chlamydomonas / Q000187:drug effects* / Q000378:metabolism; D003545:Cysteine / Q000096:biosynthesis; D005978:Glutathione / Q000096:biosynthesis; D054811:Phytochelatins / Q000096:biosynthesis; D013438:Sulfhydryl Compounds / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D013438:Sulfhydryl Compounds; D014874:Water Pollutants, Chemical; D002104:Cadmium; D054811:Phytochelatins; D005978:Glutathione; D003545:Cysteine