Crystallization and preliminary crystallographic characterization of the iron-regulated outer membrane lipoprotein FrpD from Neisseria meningitidis.
Fe-regulated protein D (FrpD) is a Neisseria meningitidis outer membrane lipoprotein that may be involved in the anchoring of the secreted repeat in toxins (RTX) protein FrpC to the outer bacterial membrane. However, the function and biological roles of the FrpD and FrpC proteins remain unknown. Native and selenomethionine-substituted variants of recombinant FrpD43-271 protein were crystallized using the sitting-drop vapour-diffusion method. Diffraction data were collected to a resolution of 2.25 A for native FrpD43-271 protein and to a resolution of 2.00 A for selenomethionine-substituted FrpD43-271 (SeMet FrpD43-271) protein. The crystals of native FrpD43-271 protein belonged to the hexagonal space group P6(2) or P6(4), while the crystals of SeMet FrpD43-271 protein belonged to the primitive orthorhombic space group P2(1)2(1)2(1).
Sviridova
Ekaterina
E
Institute of Physical Biology, University of South Bohemia Ceske Budejovice, Zamek 136, 373 33 Nove Hrady, Czech Republic.
Bumba
Ladislav
L
Rezacova
Pavlina
P
Prochazkova
Katerina
K
Kavan
Daniel
D
Bezouska
Karel
K
Kuty
Michal
M
Sebo
Peter
P
Kuta Smatanova
Ivana
I
D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D008074:Lipoproteins / Q000737:chemistry*; D009345:Neisseria meningitidis / Q000737:chemistry*
D001425:Bacterial Outer Membrane Proteins; D008074:Lipoproteins
J Biol Chem. 2004 Jun 11;279(24):24944-56
15044436
Infect Immun. 2001 Sep;69(9):5509-19
11500424
Proc Natl Acad Sci U S A. 2003 Aug 5;100(16):9542-7
12883001
N Engl J Med. 2001 May 3;344(18):1378-88
11333996
Acta Crystallogr D Biol Crystallogr. 2006 Aug;62(Pt 8):859-66
16855301
Microbes Infect. 2000 May;2(6):687-700
10884620
J Mol Biol. 1993 Jan 5;229(1):105-24
7678431
J Bacteriol. 1993 Feb;175(3):811-8
8423153
Acta Crystallogr D Biol Crystallogr. 2003 Jul;59(Pt 7):1124-30
12832754
J Mol Biol. 1968 Apr 28;33(2):491-7
5700707
Curr Top Microbiol Immunol. 2001;257:85-111
11417123
J Biol Chem. 2005 Feb 4;280(5):3251-8
15525636