title: Use of a multiplex PCR/RT-PCR approach to assess the viral causes of influenza-like illnesses in Cambodia during three consecutive dry seasons.
abstract: Acute respiratory infections are a major cause of mortality and morbidity worldwide. Using multiplex PCR/RT-PCR methods for the detection of 18 respiratory viruses, the circulation of those viruses during 3 consecutive dry seasons in Cambodia was described. Among 234 patients who presented with influenza-like illness, 35.5% were positive for at least one virus. Rhinoviruses (43.4%), parainfluenza (31.3%) viruses and coronaviruses (21.7%) were the most frequently detected viruses. Influenza A virus, parainfluenza virus 4 and SARS-coronavirus were not detected during the study period. Ninety apparently healthy individuals were included as controls and 10% of these samples tested positive for one or more respiratory viruses. No significant differences were observed in frequency and in virus copy numbers for rhinovirus detection between symptomatic and asymptomatic groups. This study raises questions about the significance of the detection of some respiratory viruses, especially using highly sensitive methods, given their presence in apparently healthy individuals. The link between the presence of the virus and the origin of the illness is therefore unclear.
authors.affiliation: Institut Pasteur in Cambodia, Phnom Penh, Cambodia.
authors.forename: Carole
authors.initials: C
authors.lastname: Buecher
authors.forename: Sek
authors.initials: S
authors.lastname: Mardy
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Veasna
authors.initials: V
authors.lastname: Duong
authors.forename: Sirenda
authors.initials: S
authors.lastname: Vong
authors.forename: Monica
authors.initials: M
authors.lastname: Naughtin
authors.forename: Astrid
authors.initials: A
authors.lastname: Vabret
authors.forename: François
authors.initials: F
authors.lastname: Freymuth
authors.forename: Vincent
authors.initials: V
authors.lastname: Deubel
authors.forename: Philippe
authors.initials: P
authors.lastname: Buchy
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002160:Cambodia / Q000453:epidemiology; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D015995:Prevalence; D012140:Respiratory Tract Diseases / Q000453:epidemiology* / Q000821:virology*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D014773:Virology / Q000379:methods*; D014777:Virus Diseases / Q000453:epidemiology* / Q000821:virology*; D014780:Viruses / Q000145:classification* / Q000302:isolation & purification; D055815:Young Adult
references.citation: Clin Infect Dis. 2005 Jun 15;40(12):1721-9
references.pmid: 15909257
references.citation: Proc Natl Acad Sci U S A. 2005 Sep 6;102(36):12891-6
references.pmid: 16118271
references.citation: J Virol Methods. 2005 Jun;126(1-2):53-63
references.pmid: 15847919
references.citation: Emerg Infect Dis. 2005 Aug;11(8):1225-9
references.pmid: 16102311
references.citation: J Clin Microbiol. 2006 Jun;44(6):2063-71
references.pmid: 16757599
references.citation: Indian Pediatr. 2008 Dec;45(12):971-5
references.pmid: 19129564
references.citation: Vaccine. 2005 Dec 30;23(50):5705-7
references.pmid: 16182415
references.citation: J Clin Microbiol. 2000 Mar;38(3):1191-5
references.pmid: 10699020
references.citation: Trop Med Int Health. 1998 Apr;3(4):268-80
references.pmid: 9623927
references.citation: Clin Infect Dis. 1998 Jun;26(6):1397-402
references.pmid: 9636869
references.citation: J Paediatr Child Health. 2008 Apr;44(4):176-81
references.pmid: 17999671
references.citation: PLoS Med. 2006 Feb;3(2):e76
references.pmid: 16401173
references.citation: Rev Infect Dis. 1990 Nov-Dec;12 Suppl 8:S870-88
references.pmid: 2270410
references.citation: J Infect Dis. 2007 Apr 1;195(7):1038-45
references.pmid: 17330795
references.citation: N Engl J Med. 2003 May 15;348(20):1953-66
references.pmid: 12690092
references.citation: J Clin Virol. 2006 Apr;35(4):388-93
references.pmid: 16458589
references.citation: J Med Virol. 2007 Aug;79(8):1238-43
references.pmid: 17597481
references.citation: Pediatr Infect Dis J. 2002 Feb;21(2):148-55
references.pmid: 11840083
references.citation: Emerg Infect Dis. 2007 Jan;13(1):165-8
references.pmid: 17370538
references.citation: N Engl J Med. 2003 May 15;348(20):1967-76
references.pmid: 12690091
references.citation: J Clin Microbiol. 2003 Sep;41(9):4298-303
references.pmid: 12958260
references.citation: J Infect Dis. 2008 Feb 1;197(3):382-9
references.pmid: 18248302
references.citation: J Clin Microbiol. 1998 May;36(5):1388-91
references.pmid: 9574711
references.citation: J Virol Methods. 2001 Sep;97(1-2):59-66
references.pmid: 11483217
references.citation: Clin Infect Dis. 2004 Sep 1;39(5):681-6
references.pmid: 15356783
references.citation: J Clin Virol. 2010 Feb;47(2):148-55
references.pmid: 20022295
references.citation: J Virol Methods. 2001 Apr;92(2):113-20
references.pmid: 11226558
references.citation: J Med Virol. 2004 Mar;72(3):484-95
references.pmid: 14748074
references.citation: Pediatr Infect Dis J. 2008 Apr;27(4):358-60
references.pmid: 18316984
references.citation: J Med Virol. 2006 Sep;78(9):1232-40
references.pmid: 16847968
references.citation: J Clin Epidemiol. 2003 Dec;56(12):1218-23
references.pmid: 14680673
references.citation: Emerg Infect Dis. 2007 Sep;13(9):1396-8
references.pmid: 18252120
references.citation: J Clin Microbiol. 2004 Oct;42(10):4632-5
references.pmid: 15472321
references.citation: J Clin Microbiol. 1999 Jan;37(1):1-7
references.pmid: 9854054
references.citation: Emerg Infect Dis. 2006 Aug;12(8):1254-6
references.pmid: 16965708
references.citation: J Clin Microbiol. 1994 Feb;32(2):484-8
references.pmid: 8150961
references.citation: J Virol Methods. 1991 Jun;33(1-2):165-89
references.pmid: 1939505
references.citation: Pediatr Allergy Immunol. 2003 Oct;14(5):363-70
references.pmid: 14641606
references.citation: Nat Med. 2001 Jun;7(6):719-24
references.pmid: 11385510
references.citation: Nat Med. 2004 Apr;10(4):368-73
references.pmid: 15034574
references.citation: J Virol. 2005 Jan;79(2):884-95
references.pmid: 15613317
references.citation: J Clin Microbiol. 2006 Mar;44(3):1132-4
references.pmid: 16517912
references.citation: Korean J Lab Med. 2007 Dec;27(6):420-7
references.pmid: 18160832
references.citation: J Infect Dis. 2004 Feb 15;189(4):706-10
references.pmid: 14767825
references.citation: Emerg Infect Dis. 2003 Jun;9(6):628-33
references.pmid: 12781000
references.citation: N Engl J Med. 2005 Apr 28;352(17):1749-59
references.pmid: 15858184
references.citation: J Med Virol. 2006 Nov;78(11):1498-504
references.pmid: 16998894
references.citation: BMC Infect Dis. 2009 Oct 15;9:168
references.pmid: 19828051
references.citation: J Med Virol. 2009 Apr;81(4):750-6
references.pmid: 19235872
references.citation: J Clin Microbiol. 2005 Dec;43(12):6176-82
references.pmid: 16333124
references.citation: Virus Res. 2002 Apr 23;85(1):41-6
references.pmid: 11955637
references.citation: J Pediatr (Rio J). 2007 Sep-Oct;83(5):422-8
references.pmid: 17940688
references.citation: Pediatr Infect Dis J. 2008 Dec;27(12):1103-7
references.pmid: 18978518