Gene copy number and polyploidy on products formation in yeast.
Yeast, such as Saccharomyces cerevisiae or Kluyveromyces lactis is appropriate strain for ethanol production or some useful compounds production. Cellulases expressing yeast can ferment ethanol from cellulosic materials; however, the productivity should be increase more and more. To improve and engineer the productivity, the target gene(s) were introduced into yeast genome. Generally, using genetic engineering, increasing integrated gene numbers are increased, the expressed protein ability such as enzymatic activities are also increased. In this mini-review, we focused on the effect of integrated gene copy number and the polyploidy on the productivity such as enzymatic activity and/or product yield.
Yamada
Ryosuke
R
Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe, Hyogo 657-8501, Japan.
Tanaka
Tsutomu
T
Ogino
Chiaki
C
Kondo
Akihiko
A
D044602:Cellulases / Q000235:genetics / Q000378:metabolism; D004171:Diploidy; D004789:Enzyme Activation; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation / Q000235:genetics; D018628:Gene Dosage; D005818:Genetic Engineering; D016681:Genome, Fungal; D006238:Haploidy; D007716:Kluyveromyces / Q000235:genetics* / Q000378:metabolism; D011123:Polyploidy; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism
D000431:Ethanol; D044602:Cellulases