Smad4 mediates malignant behaviors of human ovarian carcinoma cell through the effect on expressions of E-cadherin, plasminogen activator inhibitor-1 and VEGF.
Smad4 is involved in cancer progression and metastasis. Using a pair of human syngeneic epithelial ovarian cancer cells with low (HO-8910) and high (HO-8910PM) metastatic abilities, we aimed to reveal the role of Smad4 in ovarian cancer metastasis in vitro. Smad4 was down-regulated in HO-8910PM cell line relative to HO-8910 by implicating Smad4 was probably a potential tumor suppressor gene for ovarian cancer. Re-expression of Smad4 decreased the migration ability and inhibited the invasion capacity of HO-8910PM, while promoted the cell adhesion capacity for HO-8910PM. The stable expression of Smad4 increased the expression of E-cadherin, reduced the expression of plasminogen activator inhibitor-1 (PAI-1) and slightly down-regulated the expression of VEGF. Smad4 suppresses human ovarian cancer cell metastasis potential through its effect on the expressions of PAI-1, E-cadherin and VEGF. Results from current work implicate Smad4 might suppress the invasion and metastasis of human ovarian tumor cells through a TGF-Beta/Smad-mediated pathway.
Chen
Chen
C
Key Laboratory for Proteomics of Liaoning Province, Dalian Medical University, Dalian 116044, China.
Sun
Ming-Zhong
MZ
Liu
Shuqing
S
Yeh
Dongmei
D
Yu
Lijun
L
Song
Yang
Y
Gong
Linlin
L
Hao
Lihong
L
Hu
Jun
J
Shao
Shujuan
S
D015820:Cadherins / Q000235:genetics / Q000378:metabolism*; D002277:Carcinoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D002448:Cell Adhesion; D015536:Down-Regulation; D005260:Female; D006801:Humans; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D010051:Ovarian Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D017395:Plasminogen Activator Inhibitor 1 / Q000235:genetics / Q000378:metabolism*; D051901:Smad4 Protein / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D042442:Vascular Endothelial Growth Factors / Q000235:genetics / Q000378:metabolism*
D015820:Cadherins; D017395:Plasminogen Activator Inhibitor 1; C497796:SMAD4 protein, human; D051901:Smad4 Protein; D042442:Vascular Endothelial Growth Factors