Canine leishmaniasis in Brazil: serological follow-up of a dog population in an endemic area of american visceral leishmaniasis.
We performed a serological, clinical, and parasitological follow-up of a dog population in an endemic area of American Visceral Leishmaniasis estimated by indirect immunofluorescent assay (IFA) and western blot (WB). After twelve months, the results obtained from IFA demonstrated that 50% were seropositive and two serological profiles were observed: the first one ranging from 1/40 to 1/80 and the second >/=1/160. By WB, it was observed that the same percentage and sera from positive dogs presented the recognition of the peptides of 29 and 32 kDa up to 8 months before IFA serum conversion. Among the positive dogs, all the sera from symptomatic ones with tissue parasitism recognized the peptide of 68.5 kDa. Our results suggest the need of modifications in the control measures regarding the elimination of the dogs. They also corroborate the high sensitivity and specificity of western blot in the diagnosis of canine leishmaniasis, suggesting the possibility of its association with IFA.
da Silva
Alba Valéria Machado
AV
Departamento de Protozoologia, FIOCRUZ, Rio de Janeiro, CEP 21040-360, Brazil.
de Paula
Adelzon Assis
AA
de Pita Pereira
Daniela
D
Brazil
Reginaldo Peçanha
RP
Carreira
João Carlos Araujo
JC
Acta Trop. 2008 Jan;105(1):92-4
17953938
Vet Parasitol. 2004 Aug 6;122(4):245-52
15262002
Rev Inst Med Trop Sao Paulo. 2003 Mar-Apr;45(2):79-83
12754572
Vet J. 2001 Sep;162(2):108-20
11531395
Am J Trop Med Hyg. 1998 Feb;58(2):154-9
9502597
Trends Parasitol. 2006 Dec;22(12):552-7
17023215
Vet Parasitol. 2005 Aug 10;131(3-4):213-20
15975718
Vet Parasitol. 1999 Jul;84(1-2):33-47
10435789
Am J Trop Med Hyg. 1992 Dec;47(6):772-7
1471734
Clin Infect Dis. 1997 Nov;25(5):1240-2
9402389
Parasite Immunol. 1992 Sep;14(5):531-9
1437241
Exp Parasitol. 1996 Mar;82(2):139-46
8617340
Infect Immun. 1994 Jan;62(1):229-35
8262632
J Clin Microbiol. 2006 Apr;44(4):1318-22
16597857
J Infect Dis. 2002 Nov 1;186(9):1314-20
12402201
Am J Trop Med Hyg. 2001 Nov;65(5):510-7
11716106
J Parasitol. 1991 Aug;77(4):557-61
1865262
Rev Inst Med Trop Sao Paulo. 1966 Sep-Oct;8(5):227-35
4967348
Epidemiol Infect. 1993 Jun;110(3):647-56
8519330
Vet Parasitol. 2006 Apr 30;137(3-4):214-21
16473467
Clin Vaccine Immunol. 2006 Feb;13(2):271-6
16467337
Vet Immunol Immunopathol. 2003 Feb 10;91(3-4):161-8
12586479
Vet Immunol Immunopathol. 1999 Feb 1;67(2):117-30
10077418
Am J Trop Med Hyg. 1998 Jul;59(1):53-7
9684628
J Parasitol. 2003 Aug;89(4):832-6
14533699
Mem Inst Oswaldo Cruz. 1996 Jan-Feb;91(1):19-20
8734945