Impact of long-term storage on stability of standard DNA for nucleic acid-based methods.
Real-time PCR is dependent upon a calibration function for quantification. While long-term storage of standards saves cost and time, solutions of DNA are prone to degradation. We present here the benchmark treatment for preservation of DNA standards, involving storage in 50% glycerol-double-distilled water, whereby a deviation of 0.2 threshold cycle (C(T)) values resulted after 100 days of storage.
Röder
Barbara
B
Christian Doppler Laboratory for Molecular Food Analytics, Veterinärplatz 1, 1210 Vienna, Austria.
Frühwirth
Karin
K
Vogl
Claus
C
Wagner
Martin
M
Rossmanith
Peter
P
D019411:Clinical Laboratory Techniques / Q000379:methods / Q000592:standards*; D015925:Cryopreservation / Q000379:methods; D004247:DNA / Q000032:analysis* / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods / Q000592:standards*; D011309:Preservation, Biological / Q000379:methods*; D012015:Reference Standards; D013048:Specimen Handling / Q000379:methods*
D004247:DNA
J Hepatol. 1996 Sep;25(3):307-11
8895009
J Virol Methods. 2005 Jan;123(1):49-52
15582698
Anal Chem. 2000 Oct 15;72(20):5092-6
11055733
J Med Genet. 1990 Sep;27(9):569-70
2231649
J Pharm Sci. 2000 Jan;89(1):76-87
10664540
Biophys J. 2000 Sep;79(3):1530-6
10969014
Diagn Mol Pathol. 2007 Sep;16(3):153-7
17721323
Arch Virol. 2002 Mar;147(3):515-8
11958452
Biotechnol Bioeng. 2000 May 5;68(3):339-44
10745202
J Food Prot. 2004 Aug;67(8):1646-55
15330529
Nature. 1965 Jul 31;207(996):529-30
5886144
Res Microbiol. 2006 Oct;157(8):763-71
16814987
J Forensic Sci. 2005 May;50(3):627-9
15932097
Adv Clin Chem. 2005;40:219-59
16355924
J Pharm Sci. 2001 Mar;90(3):253-69
11170019
J Pharm Sci. 2000 Apr;89(4):443-56
10737906
Expert Rev Mol Diagn. 2009 Mar;9(2):187-97
19298142
J Vet Sci. 2003 Aug;4(2):203-4
14610378
J Clin Microbiol. 1989 Aug;27(8):1787-92
2768467
J Clin Microbiol. 1998 Jul;36(7):2117-9
9650979