title: Thermoseed hyperthermia treatment of mammary orthotopic transplantation tumors in rats and impact on immune function.
abstract: To evaluate the effect of thermoseed inductive heating on mammary orthotopic transplantation tumors and immunologic function in rats. Walker-256 tumor cells were inoculated subcutaneously into the mammary glands of Wistar rats. Rats were allocated to five treatment groups as follows: i) C group (control group); ii) M group (magnetic field group); iii) T group (thermoseed control group); iv) H1 group (hyperthermia treatment, 45 degrees C for 30 min); v) H2 group (hyperthermia treatment, 50-55 degrees C for 10 min). Immediately, 12 and 24 h after hyperthermia, two rats were sacrificed in each group for pathological and immunohistochemical examination of the expression of PCNA and HSP70. Tumor volume was measured and long-term survival was observed. The T lymphocyte subgroup IL-2 and IFN-gamma levels were measured in C, H1 and H2 groups. Both types of hyperthermia induced necrosis and apoptosis in the tumor tissue, decreased tumor volume (P<0.05), and increased survival time (P<0.01). The expression of PCNA and HSP70 in hyperthermia group was significantly different compared to the C, M and T groups (P<0.05), Hyperthermia increased CD4+ T lymphocytes and the levels of IL-2 and IFN-gamma (P<0.05). Both types of hyperthermia can suppress the growth of mammary tumors and improve immunological function of rats.
authors.affiliation: Department of Oncology, Xiangya Hospital, Central South University, Changsha 410078, P.R. China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Ouyang
authors.forename: Fuping
authors.initials: F
authors.lastname: Gao
authors.forename: Lufang
authors.initials: L
authors.lastname: Wang
authors.forename: Xiaoxue
authors.initials: X
authors.lastname: Xie
authors.forename: Fenglin
authors.initials: F
authors.lastname: Lei
authors.forename: Jumei
authors.initials: J
authors.lastname: Zhou
authors.forename: Yuping
authors.initials: Y
authors.lastname: Liao
authors.forename: Meizuo
authors.initials: M
authors.lastname: Zhong
authors.forename: Jintian
authors.initials: J
authors.lastname: Tang
mesh_terms: D000818:Animals; D002469:Cell Separation; D003300:Copper / Q000627:therapeutic use; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005434:Flow Cytometry; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis; D006979:Hyperthermia, Induced / Q000379:methods*; D007150:Immunohistochemistry; D020007:Implants, Experimental; D007371:Interferon-gamma / Q000097:blood; D007376:Interleukin-2 / Q000097:blood; D055909:Magnetic Field Therapy / Q000379:methods*; D008325:Mammary Neoplasms, Experimental / Q000097:blood / Q000276:immunology* / Q000628:therapy*; D009532:Nickel / Q000627:therapeutic use; D018809:Proliferating Cell Nuclear Antigen / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D018840:HSP70 Heat-Shock Proteins; D007376:Interleukin-2; D018809:Proliferating Cell Nuclear Antigen; C062136:nickel copper alloy; D003300:Copper; D009532:Nickel; D007371:Interferon-gamma