title: Detection of early and single infections of Schistosoma japonicum in the intermediate host snail, Oncomelania hupensis, by PCR and loop-mediated isothermal amplification (LAMP) assay.
abstract: Polymerase chain reaction (PCR) with the specific primer set amplifying 28S ribosomal DNA (rDNA) of Schistosoma japonicum was able to detect genomic DNA of S. japonicum, but not S. mansoni, at 100 fg. This procedure enabled us to detect the DNA from a single miracidium and a snail infected with one miracidium at just 1 day after infection. We compared these results with those from loop-mediated isothermal amplification (LAMP) targeting 28S rDNA and found similar results. The LAMP could amplify the specific DNA from a group of 100 normal snails mixed with one infected snail A PCR screening of infected snails from endemic regions in Anhui Province revealed schistosomal DNA even in snails found negative by microscopy. PCR and LAMP show promise for monitoring the early infection rate in snails, and they may be useful for predicting the risk of infection in the endemic places.
authors.affiliation: Section of Environmental Parasitology, Department of International Health Development, Division of Public Health, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan. tkuma.vip@tmd.ac.jp
authors.forename: Takashi
authors.initials: T
authors.lastname: Kumagai
authors.forename: Rieko
authors.initials: R
authors.lastname: Furushima-Shimogawara
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ohmae
authors.forename: Tian-Ping
authors.initials: TP
authors.lastname: Wang
authors.forename: Shaohong
authors.initials: S
authors.lastname: Lu
authors.forename: Rui
authors.initials: R
authors.lastname: Chen
authors.forename: Liyong
authors.initials: L
authors.lastname: Wen
authors.forename: Nobuo
authors.initials: N
authors.lastname: Ohta
mesh_terms: D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D004275:DNA, Ribosomal / Q000235:genetics; D051379:Mice; D008813:Mice, Inbred ICR; D016133:Polymerase Chain Reaction / Q000379:methods*; D012549:Schistosoma japonicum / Q000235:genetics / Q000302:isolation & purification*; D012908:Snails / Q000469:parasitology*
chemical_list: D017931:DNA Primers; D004275:DNA, Ribosomal
references.citation: Nucleic Acids Res. 2000 Jun 15;28(12):E63
references.pmid: 10871386
references.citation: Mol Cell Probes. 1995 Jun;9(3):161-5
references.pmid: 7477008
references.citation: N Engl J Med. 2002 Apr 18;346(16):1212-20
references.pmid: 11961151
references.citation: Theriogenology. 2004 Sep 1;62(5):887-96
references.pmid: 15251240
references.citation: Parasitology. 2006 Nov;133(Pt 5):581-7
references.pmid: 16834820
references.citation: Acta Trop. 2005 Nov-Dec;96(2-3):69-96
references.pmid: 16312039
references.citation: Exp Parasitol. 2009 Feb;121(2):175-9
references.pmid: 19027005
references.citation: Mol Biol Evol. 2005 Nov;22(11):2157-65
references.pmid: 16014872
references.citation: Parasitology. 2005 Oct;131(Pt 4):497-500
references.pmid: 16174414
references.citation: Chin Med J (Engl). 2009 May 5;122(9):1009-14
references.pmid: 19493433
references.citation: Int J Parasitol. 2010 Mar 1;40(3):327-31
references.pmid: 19735662
references.citation: Int J Parasitol. 2002 Aug;32(9):1163-74
references.pmid: 12117499
references.citation: Acta Trop. 2005 Nov-Dec;96(2-3):255-65
references.pmid: 16154104
references.citation: Am J Trop Med Hyg. 2001 Dec;65(6):907-11
references.pmid: 11791997
references.citation: N Engl J Med. 2009 Jan 8;360(2):121-8
references.pmid: 19129526
references.citation: Emerg Infect Dis. 2007 Oct;13(10):1470-6
references.pmid: 18257989
references.citation: Exp Parasitol. 2006 Oct;114(2):84-8
references.pmid: 16571353
references.citation: Acta Trop. 2007 Jun;102(3):182-9
references.pmid: 17574198
references.citation: Parasitology. 1995 Aug;111 ( Pt 2):167-75
references.pmid: 7675531
references.citation: Acta Trop. 2005 Nov-Dec;96(2-3):198-204
references.pmid: 16188215
references.citation: Parasit Vectors. 2009 Mar 15;2(1):15
references.pmid: 19284882
references.citation: Am J Trop Med Hyg. 2009 Oct;81(4):578-82
references.pmid: 19815869
references.citation: J Parasitol. 2006 Oct;92(5):1088-91
references.pmid: 17152955
references.citation: Appl Environ Microbiol. 2007 Sep;73(17):5660-2
references.pmid: 17616628
references.citation: Trop Med Int Health. 2009 Dec;14(12):1475-83
references.pmid: 19793080
references.citation: Bull World Health Organ. 2006 Feb;84(2):139-44
references.pmid: 16501732
references.citation: Parasitol Int. 2006 Sep;55(3):213-8
references.pmid: 16822708
references.citation: Acta Trop. 2005 Nov-Dec;96(2-3):97-105
references.pmid: 16125655
references.citation: Mol Biochem Parasitol. 1985 May;15(2):215-30
references.pmid: 4010707
references.citation: Diagn Microbiol Infect Dis. 2003 Sep;47(1):349-58
references.pmid: 12967749
references.citation: Biochem Biophys Res Commun. 2008 Nov 28;376(4):671-6
references.pmid: 18809384
references.citation: J Clin Microbiol. 2007 Aug;45(8):2521-8
references.pmid: 17567794
references.citation: Am J Trop Med Hyg. 2002 Feb;66(2):157-62
references.pmid: 12135287
references.citation: PLoS Negl Trop Dis. 2008;2(11):e337
references.pmid: 19015722
references.citation: J Clin Microbiol. 2009 Jan;47(1):168-74
references.pmid: 19005142
references.citation: Clin Microbiol Rev. 2001 Apr;14(2):270-95
references.pmid: 11292639
references.citation: Acta Trop. 2004 Jun;91(1):43-6
references.pmid: 15158687
references.citation: Exp Parasitol. 2009 May;122(1):47-50
references.pmid: 19545521
references.citation: Acta Trop. 2008 Jan;105(1):74-80
references.pmid: 18036505