title: Upregulation of coronary endothelial P-selectin in a monkey heart ischemia reperfusion model.
abstract: The design of targeted ultrasound contrast agents for molecular imaging of myocardial ischemia-reperfusion (IR) requires the availability of an adequate in vivo model in a species in which cross reactivity with the target occurs. P-selectin (Psel) is an activation-dependent endothelial receptor that supports rapid and reversible cell adhesion in a flowing system. Together with E- and L-selectins it constitutes the selectin family of adhesion molecules. We investigated the myocardial expression of selectins in a closed chest minimally invasive monkey myocardial IR model. Catheter-based occlusion (30-50 min) followed by reperfusion (3 or 5 h) of left anterior descending artery (LAD) was performed in anesthetised monkeys. At the end of each procedure animals were killed, and their hearts were excised. The tissues were analyzed immunohistochemically using an anti-human Psel antibody (AK-6 clone) that cross reacts with rhesus monkey. Histopathological features confirm the presence of IR injuries in myocardial tissues. There was significant increase in the Psel expression in vessels from the IR areas. However, significantly higher Psel immunoreactivity was also seen in areas which are distant from IR injuries.
authors.affiliation: Ernst Felder Laboratories, Discovery Biology, Bracco Research USA, 305 College Road East, Princeton, NJ 08540, USA. Regi_T@yahoo.com
authors.forename: Regi
authors.initials: R
authors.lastname: Thomas
authors.forename: Yulan
authors.initials: Y
authors.lastname: Cheng
authors.forename: Jun
authors.initials: J
authors.lastname: Yan
authors.forename: Thierry
authors.initials: T
authors.lastname: Bettinger
authors.forename: Anne
authors.initials: A
authors.lastname: Broillet
authors.forename: Gilles
authors.initials: G
authors.lastname: Rioufol
authors.forename: Adrian D
authors.initials: AD
authors.lastname: Nunn
mesh_terms: D000818:Animals; D000906:Antibodies; D000918:Antibody Specificity; D001792:Blood Platelets / Q000378:metabolism; D002460:Cell Line; D003429:Cross Reactions; D004195:Disease Models, Animal; D042783:Endothelial Cells / Q000378:metabolism / Q000473:pathology; D004730:Endothelium, Vascular / Q000378:metabolism* / Q000473:pathology*; D000882:Haplorhini; D006439:Hemodynamics; D006801:Humans; D042601:Lymphatic Vessels / Q000378:metabolism; D015428:Myocardial Reperfusion Injury / Q000378:metabolism* / Q000473:pathology* / Q000503:physiopathology; D009206:Myocardium / Q000378:metabolism / Q000473:pathology; D019007:P-Selectin / Q000378:metabolism*; D021381:Protein Transport; D015854:Up-Regulation
chemical_list: D000906:Antibodies; D019007:P-Selectin; C546937:SELP protein, human
references.citation: Cell. 1993 Aug 13;74(3):541-54
references.pmid: 7688665
references.citation: Heart Lung Circ. 2007 Apr;16(2):85-92
references.pmid: 17314070
references.citation: Ann Thorac Surg. 2005 Jan;79(1):204-11
references.pmid: 15620944
references.citation: Cell. 1996 Oct 4;87(1):13-20
references.pmid: 8858144
references.citation: J Cell Biol. 2003 Dec 22;163(6):1385-95
references.pmid: 14676308
references.citation: FASEB J. 1995 Jul;9(10):874-82
references.pmid: 7615157
references.citation: Cardiovasc Res. 1999 Sep;43(4):860-78
references.pmid: 10615413
references.citation: J Leukoc Biol. 1999 Mar;65(3):299-306
references.pmid: 10080531
references.citation: Eur Heart J. 2007 Aug;28(16):2011-7
references.pmid: 17526905
references.citation: Langmuir. 2007 Nov 20;23(24):12261-8
references.pmid: 17949112
references.citation: Am J Respir Cell Mol Biol. 1990 Jun;2(6):487-9
references.pmid: 2189454
references.citation: J Leukoc Biol. 1993 May;53(5):511-7
references.pmid: 8099114
references.citation: Biomed Microdevices. 2007 Apr;9(2):207-14
references.pmid: 17160704
references.citation: Mol Pharm. 2006 Sep-Oct;3(5):516-24
references.pmid: 17009850
references.citation: Am J Physiol. 1997 May;272(5 Pt 1):G1195-200
references.pmid: 9176230
references.citation: Am J Physiol. 1999 May;276(5):H1567-73
references.pmid: 10330240
references.citation: J Cell Biol. 1991 Oct;115(1):223-34
references.pmid: 1717478
references.citation: J Control Release. 2006 Sep 12;114(3):288-99
references.pmid: 16887229
references.citation: Cardiovasc Res. 2010 Jul 15;87(2):291-9
references.pmid: 20299333
references.citation: Biophys J. 1997 Jun;72(6):2820-33
references.pmid: 9168056
references.citation: BMC Evol Biol. 2007 Sep 14;7:166
references.pmid: 17868453
references.citation: Arterioscler Thromb Vasc Biol. 2010 Jan;30(1):54-9
references.pmid: 19834105
references.citation: FASEB J. 1990 Dec;4(15):3355-9
references.pmid: 2253850
references.citation: J Am Coll Cardiol. 2000 Jul;36(1):122-9
references.pmid: 10898423
references.citation: J Clin Invest. 1993 Jun;91(6):2620-9
references.pmid: 7685773
references.citation: Zhonghua Yi Xue Za Zhi. 2009 Jun 23;89(24):1698-701
references.pmid: 19957530
references.citation: J Endourol. 2009 Mar;23(3):373-8
references.pmid: 19245294
references.citation: Semin Vasc Med. 2003 May;3(2):147-62
references.pmid: 15199478
references.citation: J Biol Chem. 1989 May 25;264(15):9053-60
references.pmid: 2470750
references.citation: Circulation. 1995 Mar 1;91(5):1520-4
references.pmid: 7867193
references.citation: JAMA. 1999 Dec 1;282(21):2035-42
references.pmid: 10591386
references.citation: J Leukoc Biol. 1995 Jan;57(1):45-55
references.pmid: 7530283
references.citation: Biochim Biophys Acta. 2000 Jun 2;1497(1):1-10
references.pmid: 10838154
references.citation: J Clin Invest. 1993 Oct;92(4):2042-7
references.pmid: 7691890
references.citation: Thromb Res. 1997 Jan 15;85(2):159-64
references.pmid: 9058490
references.citation: Cardiovasc Res. 2004 Feb 15;61(3):498-511
references.pmid: 14962480