title: Relating the physicochemical characteristics and dispersion of multiwalled carbon nanotubes in different suspension media to their oxidative reactivity in vitro and inflammation in vivo.
abstract: Reactive oxygen species (ROS) production is important in the toxicity of pathogenic particles such as fibres. We examined the oxidative potential of straight (50 microm and 10 microm) and tangled carbon nanotubes in a cell free assay, in vitro and in vivo using different dispersants. The cell free oxidative potential of tangled nanotubes was higher than for the straight fibres. In cultured macrophages tangled tubes exhibited significantly more ROS at 30 min, while straight tubes increased ROS at 4 h. ROS was significantly higher in bronchoalveolar lavage cells of animals instilled with tangled and 10 mum straight fibres, whereas the number of neutrophils increased only in animals treated with the long tubes. Addition of dispersants in the suspension media lead to enhanced ROS detection by entangled tubes in the cell-free system. Tangled fibres generated more ROS in a cell-free system and in cultured cells, while straight fibres generated a slower but more prolonged effect in animals.
authors.affiliation: Biomedicine and Sport Science Research Group, School of Life Sciences, Edinburgh Napier University, Edinburgh, Scotland, UK.
authors.forename: Barbara
authors.initials: B
authors.lastname: Rothen-Rutishauser
authors.forename: David M
authors.initials: DM
authors.lastname: Brown
authors.forename: Matthew
authors.initials: M
authors.lastname: Piallier-Boyles
authors.forename: Ian A
authors.initials: IA
authors.lastname: Kinloch
authors.forename: Alan H
authors.initials: AH
authors.lastname: Windle
authors.forename: Peter
authors.initials: P
authors.lastname: Gehr
authors.forename: Vicki
authors.initials: V
authors.lastname: Stone
mesh_terms: D000818:Animals; D018893:Bronchoalveolar Lavage; D002460:Cell Line; D006801:Humans; D007249:Inflammation / Q000139:chemically induced*; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D008297:Male; D051379:Mice; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000633:toxicity; D010084:Oxidation-Reduction; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D037742:Nanotubes, Carbon; D017382:Reactive Oxygen Species