Analytical ultracentrifugation of colloids.
In this contribution the use of Analytical Ultracentrifugation (AUC) for the modern analysis of colloids is reviewed. Since AUC is a fractionation technique, distributions of the sedimentation coefficient, particle size and shape, molar mass and density can be obtained for particle sizes spanning the entire colloidal range. The Ångström resolution and the reliable statistics with which particle size distributions can be obtained from analytical ultracentrifugation makes this a high resolution analysis technique for the characterization of nanoparticles in solution or suspension. Several examples showing successful applications of AUC to complex problems in colloid science are given to illustrate the broad range and versatility of questions that can be answered by AUC experiments.
Planken
Karel L
KL
Max-Planck-Institute of Colloids and Interfaces, Colloid Chemistry, Research Campus Golm, Am Mühlenberg, D-14424 Potsdam, Germany. karel.planken@mpikg.mpg.de
Cölfen
Helmut
H
D001703:Biophysics / Q000379:methods; D002623:Chemistry Techniques, Analytical; D002845:Chromatography / Q000379:methods; D003102:Colloids / Q000737:chemistry*; D003201:Computers; D004247:DNA / Q000737:chemistry; D046911:Macromolecular Substances / Q000737:chemistry; D015233:Models, Statistical; D055095:Optics and Photonics; D010316:Particle Size; D012984:Software; D013997:Time Factors; D014461:Ultracentrifugation
D003102:Colloids; D046911:Macromolecular Substances; D004247:DNA