Urinary excretion of nephrin in patients with severe preeclampsia. Urinary nephrin in preeclampsia.
OBJECTIVE
The objective of this study was to examine whether nephrin is present in the urine of patients with severe preeclampsia.


METHODS
A total of 45 women were recruited for this study, and 25 of these patients had severe preeclampsia. Twenty gestational age-matched normotensive women without proteinuria served as a control group. Urine samples were collected close to delivery, typically ≤24 h before delivery. Western blot analysis was performed to assess the excretion of nephrin in urine.


RESULTS
Nephrin was detected in all urine samples from all the women with severe preeclampsia but not in urine from normotensive controls.


CONCLUSIONS
In pregnancy complicated by severe preeclampsia, urinary nephrin shedding, reflecting the damage in the glomerular slit diaphragm, was observed.
Son
Ga Hyun
GH
Division of Maternal-Fetal Medicine, Department of Obstetrics and Gynecology, Yonsei University Health System, Seoul, Korea.
Kim
Jeong Hyeon
JH
Hwang
Jung Hae
JH
Kim
Young Han
YH
Park
Yong Won
YW
Kwon
Ja Young
JY
D000328:Adult; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D008565:Membrane Proteins / Q000652:urine*; D011225:Pre-Eclampsia / Q000652:urine*; D011247:Pregnancy; D011296:Prenatal Diagnosis; D012720:Severity of Illness Index
D008565:Membrane Proteins; C113267:nephrin