Pulmonary thrombosis in the mouse following intravenous administration of quantum dot-labeled mesenchymal cells.
Quantum dots (QDs) are emerging as novel diagnostic agents. Yet, only a few studies have examined the possible deleterious effects of QD-labeled stem cells. We assessed the potential toxic effects of QD-labeled human embryonic palatal mesenchymal (QD-HEPM) cells in male NOD/SCID mice for six months, following the administration of a single intravenous injection. Control animals were administered with non-labeled HEPM cells. No treatment-related clinical signs, hematological, or biochemical parameters were found in the QD-HEPM animals in comparison to control animals. Histologically, multifocal organizing thrombi were noted in the pulmonary arteries of all QD-HEPM animals from the one-week study group and in one animal from the one-month group. Additionally, increased severity of perivascular inflammation was noted at the injection sites of QD-HEPM animals from the one-week group. This is the first study reporting histopathological evidence for pro-thrombotic adverse effects mediated by QD labeling.
Ramot
Yuval
Y
Hadassah-Hebrew University Medical Center, Jerusalem, Israel.
Steiner
Michal
M
Morad
Vered
V
Leibovitch
Sigalit
S
Amouyal
Netanel
N
Cesta
Mark F
MF
Nyska
Abraham
A
D000818:Animals; D017690:Cell Transplantation / Q000009:adverse effects*; D006801:Humans; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D011651:Pulmonary Artery / Q000473:pathology; D011655:Pulmonary Embolism / Q000209:etiology* / Q000473:pathology; D045663:Quantum Dots