title: Overexpression of the ethylene-responsive factor gene BrERF4 from Brassica rapa increases tolerance to salt and drought in Arabidopsis plants.
abstract: Ethylene-responsive factors (ERFs), within a subgroup of the AP2/ERF transcription factor family, are involved in diverse plant reactions to biotic or abiotic stresses. Here, we report that overexpression of an ERF gene from Brassica rapa ssp. pekinensis (BrERF4) led to improved tolerance to salt and drought stresses in Arabidopsis. It also significantly affected the growth and development of transgenic plants. We detected that salt-induced expressions of a transcriptional repressor gene, AtERF4, and some Ser/Thr protein phosphatase2C genes, ABI1, ABI2 and AtPP2CA, were suppressed in BrERF4-overexpressing Arabidopsis plants. Furthermore, BrERF4 was induced by treatment with ethylene or methyljasmonate, but not by abscisic acid or NaCl in B. rapa. These results suggest that BrERF4 is activated through a network of different signaling pathways in response to salinity and drought.
authors.affiliation: School of Applied Biosciences, Kyungpook National University, Daegu, 702-701, Korea.
authors.forename: Yean Joo
authors.initials: YJ
authors.lastname: Seo
authors.forename: Jong-Beum
authors.initials: JB
authors.lastname: Park
authors.forename: Yeon-Jeong
authors.initials: YJ
authors.lastname: Cho
authors.forename: Choonkyun
authors.initials: C
authors.lastname: Jung
authors.forename: Hak Soo
authors.initials: HS
authors.lastname: Seo
authors.forename: Soon-Ki
authors.initials: SK
authors.lastname: Park
authors.forename: Baek Hie
authors.initials: BH
authors.lastname: Nahm
authors.forename: Jong Tae
authors.initials: JT
authors.lastname: Song
mesh_terms: D000085:Acetates / Q000494:pharmacology; D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D029741:Brassica rapa / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D048708:Cell Growth Processes / Q000235:genetics; D002478:Cells, Cultured; D003517:Cyclopentanes / Q000494:pharmacology; D005030:Ethylenes / Q000494:pharmacology; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D054883:Oxylipins / Q000494:pharmacology; D010937:Plant Growth Regulators / Q000494:pharmacology; D030821:Plants, Genetically Modified; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D055049:Salt Tolerance / Q000235:genetics; D013312:Stress, Physiological / Q000187:drug effects / Q000235:genetics; D019076:Transgenes / Q000235:genetics
chemical_list: D000085:Acetates; D029681:Arabidopsis Proteins; D003517:Cyclopentanes; C505622:ERF4 protein, Arabidopsis; D005030:Ethylenes; D054883:Oxylipins; D010937:Plant Growth Regulators; D012097:Repressor Proteins; C072239:methyl jasmonate; C036216:ethylene