title: Effects of hypophyseal or thymic allograft on thymus development in partially decerebrate chicken embryos: expression of PCNA and CD3 markers.
abstract: Changes in chicken embryo thymus after partial decerebration (including the hypophysis) and after hypophyseal or thymic allograft were investigated. Chicken embryos were partially decerebrated at 36-40 hr of incubation and on day 12 received a hypophysis or a thymus allograft from 18-day-old donor embryos. The thymuses of normal, sham-operated and partially decerebrate embryos were collected on day 12 and 18. The thymuses of the grafted embryos were collected on day 18. The samples were examined with histological method and tested for the anti-PCNA and anti-CD3 immune-reactions. After partial decerebration, the thymic cortical and medullary compartments diminished markedly in size. Anti-PCNA and anti-CD3 revealed a reduced immune-reaction, verified also by statistical analysis. In hypophyseal or grafted embryos, the thymic morphological compartments improved, the anti-PCNA and anti-CD3 immune-reactions recovered much better after the thymic graft, probably due to the thymic growth factors and also by an emigration of thymocytes from the same grafted thymus.
authors.affiliation: Department of Physiology and Pharmacology Vittorio Erspamer, Faculty of Medicine, University La Sapienza, Piazzale A. Moro 5, Rome, Italy. mariangela.aita@uniroma1.it
authors.forename: M
authors.initials: M
authors.lastname: Aita
authors.forename: F
authors.initials: F
authors.lastname: Benedetti
authors.forename: E
authors.initials: E
authors.lastname: Carafelli
authors.forename: E
authors.initials: E
authors.lastname: Caccia
authors.forename: N
authors.initials: N
authors.lastname: Romano
mesh_terms: D000818:Animals; D017252:CD3 Complex / Q000378:metabolism*; D002642:Chick Embryo; D018507:Gene Expression Regulation, Developmental; D007030:Hypothalamo-Hypophyseal System / Q000502:physiology; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism*; D013194:Staining and Labeling; D013601:T-Lymphocytes / Q000276:immunology*; D013950:Thymus Gland / Q000033:anatomy & histology / Q000254:growth & development* / Q000637:transplantation; D014184:Transplantation, Homologous
chemical_list: D017252:CD3 Complex; D018809:Proliferating Cell Nuclear Antigen
references.citation: J Vet Med Sci. 1997 Jul;59(7):601-3
references.pmid: 9271458
references.citation: J Cell Biol. 1987 Oct;105(4):1549-54
references.pmid: 2889739
references.citation: Clin Immunol Immunopathol. 1981 Jan;18(1):108-20
references.pmid: 7460394
references.citation: Science. 1990 Jun 15;248(4961):1335-41
references.pmid: 1972592
references.citation: Thymus. 1991 May;17(3):155-65
references.pmid: 1712130
references.citation: Eur J Histochem. 2001;45(2):191-202
references.pmid: 11512640
references.citation: Cell Mol Biol. 1989;35(2):137-45
references.pmid: 2731191
references.citation: Semin Immunol. 2000 Oct;12(5):429-33
references.pmid: 11085175
references.citation: J Neuroimmunol. 1997 Jan;72(1):67-74
references.pmid: 9003246
references.citation: Gen Comp Endocrinol. 1967 Oct;9(2):172-86
references.pmid: 6069718
references.citation: Anat Embryol (Berl). 1993 Oct;188(4):339-44
references.pmid: 8279698
references.citation: Endocrinology. 2009 Sep;150(9):3977-9
references.pmid: 19700607
references.citation: Biochemistry. 1986 Jan 14;25(1):26-36
references.pmid: 3954990
references.citation: Exp Cell Res. 1981 Dec;136(2):311-9
references.pmid: 7308310
references.citation: Eur J Histochem. 2006 Jan-Mar;50(1):69-78
references.pmid: 16584987
references.citation: Histochem J. 1998 Feb;30(2):61-7
references.pmid: 10192545
references.citation: Endocr Rev. 1996 Feb;17(1):64-102
references.pmid: 8641224
references.citation: Cell Tissue Res. 1996 Oct;286(1):69-80
references.pmid: 8781214
references.citation: Am J Anat. 1991 May;191(1):57-66
references.pmid: 1648305
references.citation: Anat Rec A Discov Mol Cell Evol Biol. 2005 Feb;282(2):106-9
references.pmid: 15635673
references.citation: J Exp Med. 1994 Jun 1;179(6):1835-46
references.pmid: 8195711
references.citation: J Neuroendocrinol. 1999 Sep;11(9):685-92
references.pmid: 10447807
references.citation: Histochemistry. 1984;80(3):207-11
references.pmid: 6373680
references.citation: Physiol Rev. 1989 Jan;69(1):1-32
references.pmid: 2536183
references.citation: Curr Top Pathol. 1989;79:59-99
references.pmid: 2644087
references.citation: J Immunol. 1989 Jan 1;142(1):57-66
references.pmid: 2783327
references.citation: Basic Appl Histochem. 1986;30(1):53-9
references.pmid: 3521577
references.citation: J Immunol. 1987 Jun 15;138(12):4114-20
references.pmid: 2884260
references.citation: Endocr Rev. 2000 Aug;21(4):412-43
references.pmid: 10950159
references.citation: Mol Cell Neurosci. 1990 Aug;1(1):10-9
references.pmid: 19912750
references.citation: FEBS Lett. 1984 Apr 24;169(2):185-8
references.pmid: 6143692
references.citation: Endocrinology. 1989 Aug;125(2):1025-36
references.pmid: 2546732
references.citation: Dev Immunol. 1995;4(3):211-26
references.pmid: 8770560
references.citation: Dev Immunol. 1992;2(1):19-27
references.pmid: 1387828
references.citation: Adv Immunol. 1991;50:87-117
references.pmid: 1835268
references.citation: Immunol Today. 1995 Aug;16(8):365-70
references.pmid: 7546190
references.citation: Histochem J. 1992 Mar;24(3):121-31
references.pmid: 1349881
references.citation: J Endocrinol. 2006 Apr;189(1):45-55
references.pmid: 16614380
references.citation: J Immunol. 1988 Apr 1;140(7):2133-8
references.pmid: 3258329
references.citation: J Biol Chem. 1986 Sep 15;261(26):12310-6
references.pmid: 3745189
references.citation: Exp Cell Res. 1985 Feb;156(2):450-4
references.pmid: 2857125
references.citation: Am J Anat. 1984 Jul;170(3):261-99
references.pmid: 6383002
references.citation: Curr Top Microbiol Immunol. 1996;212:79-93
references.pmid: 8934812
references.citation: Eur J Histochem. 1995;39(4):289-300
references.pmid: 8835183
references.citation: Anat Embryol (Berl). 1996 Jun;193(6):593-600
references.pmid: 8737815
references.citation: Immunology. 1988 Apr;63(4):721-7
references.pmid: 3284819
references.citation: Nature. 1984 May 24-30;309(5966):374-6
references.pmid: 6145097
references.citation: Acta Anat (Basel). 1994;150(2):96-103
references.pmid: 7976198
references.citation: Rev Physiol Biochem Pharmacol. 1982;92:115-91
references.pmid: 7038822
references.citation: Poult Sci. 1994 Jul;73(7):979-83
references.pmid: 7937486
references.citation: J Immunol. 1993 Apr 1;150(7):2746-52
references.pmid: 8095955
references.citation: Biochimie. 1990 Sep;72(9):653-60
references.pmid: 2126205
references.citation: Thymus. 1991 Mar;17(2):115-22
references.pmid: 2028505
references.citation: Eur J Histochem. 2007 Oct-Dec;51(4):241-50
references.pmid: 18162453
references.citation: Dev Immunol. 1992;2(1):51-66
references.pmid: 1387829
references.citation: Arch Histol Cytol. 1996 May;59(2):197-203
references.pmid: 8790865
references.citation: Dev Comp Immunol. 1998 May-Jun;22(3):279-87
references.pmid: 9700458
references.citation: Exp Cell Res. 1986 Apr;163(2):287-93
references.pmid: 2869964
references.citation: Integr Comp Biol. 2006 Dec;46(6):1060-71
references.pmid: 21672808
references.citation: Int Immunol. 2001 Jun;13(6):769-76
references.pmid: 11369704
references.citation: Science. 1989 May 26;244(4907):943-50
references.pmid: 2658058
references.citation: Nature. 1987 Apr 2-8;326(6112):515-7
references.pmid: 2882423
references.citation: Arch Int Physiol Biochim. 1988 Aug;96(3):A2-26
references.pmid: 2463798
references.citation: Neuroimmunomodulation. 1999 Jan-Apr;6(1-2):137-42
references.pmid: 9876244
references.citation: Eur J Histochem. 2009 Sep 23;53(3):167-76
references.pmid: 19864211
references.citation: J Cell Sci. 1990 May;96 ( Pt 1):121-9
references.pmid: 1695635
references.citation: Dev Comp Immunol. 1980 Spring;4(2):191-209
references.pmid: 6995188