title: [Investigation of metallo-beta-lactamase-producing Acinetobacter sp and Pseudomonas aeruginosa at an emergency hospital in Porto Alegre, State of Rio Grande do Sul, Brazil].
abstract: BACKGROUND
  The appearance of metallo-beta-lactamase (MBL)-producing Pseudomonas aeruginosa and Acinetobacter sp. is a challenge for hospitals.
  
  
  METHODS
  The production of MBL in clinical isolates of Pseudomonas aeruginosa and Acinetobacter sp. From an emergency hospital in Porto Alegre was investigated using the disk approximation test and MBL E-test. The bla genes were determined using PCR.
  
  
  RESULTS
  Two strains of Pseudomonas aeruginosa and eight of Acinetobacter sp were shown to be MBL phenotypes. Amplification of the blaSPM-1 gene confirmed the presence of the enzyme in P. aeruginosa.
  
  
  CONCLUSIONS
  Caution is needed in evaluating phenotype tests used for routine detection of metallo-beta-lactamases.
authors.affiliation: Instituto de Ciências da Saúde, Universidade Feevale, Novo Hamburgo, RS.
authors.forename: Vani Dos Santos
authors.initials: Vdos S
authors.lastname: Laranjeira
authors.forename: Desiree Padilha
authors.initials: DP
authors.lastname: Marchetti
authors.forename: Juçara Rodrigues
authors.initials: JR
authors.lastname: Steyer
authors.forename: Gertrudes
authors.initials: G
authors.lastname: Corção
authors.forename: Simone Ulrich
authors.initials: SU
authors.lastname: Picoli
mesh_terms: D000150:Acinetobacter / Q000187:drug effects / Q000201:enzymology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001938:Brazil; D005798:Genes, Bacterial / Q000235:genetics*; D006801:Humans; D008826:Microbial Sensitivity Tests; D010641:Phenotype; D016133:Polymerase Chain Reaction; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000201:enzymology*; D001618:beta-Lactamases / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D001618:beta-Lactamases