Crystallization and preliminary X-ray studies of a galactose-specific lectin from the seeds of bitter gourd (Momordica charantia).
A galactose-specific lectin from the seeds of bitter gourd (Momordica charantia) is a four-chain type II ribosome-inactivating protein (RIP) resulting from covalent association through a disulfide bridge between two identical copies of a two-chain unit. The available structural information on such four-chain RIPs is meagre. The bitter gourd lectin was therefore crystallized for structural investigation and the crystals have been characterized. It is anticipated that the structure of the orthorhombic crystals will be analysed using molecular replacement by taking advantage of its sequence, and presumably structural, homology to normal two-chain type II RIPs.
Chandran
Thyageshwar
T
Molecular Biophysics Unit, Indian Institute of Science, Bangalore 560 012, India.
Sharma
Alok
A
Vijayan
M
M
D000595:Amino Acid Sequence; D003460:Crystallization; D018360:Crystallography, X-Ray; D005690:Galactose / Q000737:chemistry*; D037102:Lectins / Q000737:chemistry*; D008969:Molecular Sequence Data; D029777:Momordica charantia / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry; D016415:Sequence Alignment; D013379:Substrate Specificity
D037102:Lectins; D005690:Galactose
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