Two antifungal components isolated from Fructus Psoraleae and Folium Eucalypti Globuli by bioassay-guided purification.
Fructus Psoraleae and Folium Eucalypti Globuli have long been used as Chinese medicines to treat various ailments such as asthma, eczema and dermatomycosis. In previous studies, their antifungal activities were demonstrated. The aim of the present study was to isolate active antidermatophytic compounds from their ethanolic extracts by means of bioassay-guided purification. Guided by the inhibitory activities on Trichophyton mentagrophytes, Trichophyton rubrum and Paecilomyces variotii, bakuchiol was isolated from the n-hexane fraction of Fructus Psoraleae whilst macrocarpal C was isolated from the n-hexane fraction of Folium Eucalypti Globuli. Both pure compounds could effectively inhibit the growth of dermatophytes in vitro. This is the first paper to report the isolation and identification of active antidermatophytic compounds from Fructus Psoraleae and Folium Eucalypti Globuli by the bioassay-guided purification.
Lau
Kit-Man
KM
Institute of Chinese Medicine, The Chinese University of Hong Kong, Hong Kong.
Fu
Lai-Hong
LH
Cheng
Ling
L
Wong
Chun-Wai
CW
Wong
Yin-Lai
YL
Lau
Ching-Po
CP
Han
Simon Quan-Bin
SQ
Chan
Paul Kay-Sheung
PK
Fung
Kwok-Pui
KP
Lau
Clara Bik-San
CB
Hui
Mamie
M
Leung
Ping-Chung
PC
D000935:Antifungal Agents / Q000302:isolation & purification / Q000494:pharmacology*; D003883:Arthrodermataceae / Q000187:drug effects*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology*; D005052:Eucalyptus / Q000737:chemistry*; D005638:Fruit; D010143:Paecilomyces / Q000187:drug effects; D010636:Phenols / Q000302:isolation & purification / Q000494:pharmacology*; D010696:Phloroglucinol / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000494:pharmacology; D018515:Plant Leaves; D029907:Psoralea / Q000737:chemistry*; D012717:Sesquiterpenes / Q000302:isolation & purification / Q000494:pharmacology*; D014249:Trichophyton / Q000187:drug effects
D000935:Antifungal Agents; D004365:Drugs, Chinese Herbal; D010636:Phenols; D012717:Sesquiterpenes; C518773:macrocarpal C; D010696:Phloroglucinol; C012765:bakuchiol