title: Auto-mobilized adult hematopoietic stem cells advance neovasculature in diabetic retinopathy of mice.
abstract: BACKGROUND
  Hematopoietic stem cells (HSCs) can be used to deliver functionally active angiostatic molecules to the retinal vasculature by targeting active astrocytes and may be useful in targeting pre-angiogenic retinal lesions. We sought to determine whether HSC mobilization can ameliorate early diabetic retinopathy in mice.
  
  
  METHODS
  Mice were devided into four groups: normal mice control group, normal mice HSC-mobilized group, diabetic mice control group and diabetic mice HSC mobilized group. Murine stem cell growth factor (murine SCF) and recombined human granulocyte colony stimulating factor (rhG-csf) were administered to the mice with diabetes and without diabetes for continuous 5 days to induce autologous HSCs mobilization, and subcutaneous injection of physiological saline was used as control. Immunohistochemical double staining was conducted with anti-mouse rat CD31 monoclonal antibody and anti-BrdU rat antibody.
  
  
  RESULTS
  Marked HSCs clearly increased after SCF plus G-csf-mobilization. Non-mobilized diabetic mice showed more HSCs than normal mice (P=0.032), and peripheral blood significantly increased in both diabetic and normal mice (P=0.000). Diabetic mice showed more CD31 positive capillary vessels (P=0.000) and accelerated endothelial cell regeneration. Only diabetic HSC-mobilized mice expressed both BrdU and CD31 antigens in the endothelial cells of new capillaries.
  
  
  CONCLUSIONS
  Auto-mobilized adult hematopoietic stem cells advance neovasculature in diabetic retinopathy of mice.
authors.affiliation: Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Ophthalmology & Visual Science Key Laboratory, Beijing 100730, China.
authors.forename: Bei
authors.initials: B
authors.lastname: Tian
authors.forename: Xiao-xin
authors.initials: XX
authors.lastname: Li
authors.forename: Li
authors.initials: L
authors.lastname: Shen
authors.forename: Min
authors.initials: M
authors.lastname: Zhao
authors.forename: Wen-zhen
authors.initials: WZ
authors.lastname: Yu
mesh_terms: D000818:Animals; D003930:Diabetic Retinopathy / Q000188:drug therapy*; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000627:therapeutic use; D016298:Hematopoietic Cell Growth Factors / Q000627:therapeutic use; D019650:Hematopoietic Stem Cell Mobilization / Q000379:methods*; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism
chemical_list: D016298:Hematopoietic Cell Growth Factors; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D016179:Granulocyte Colony-Stimulating Factor