title: Multinucleated giant cells with an osteoclast phenotype derived from caprine peripheral blood mononuclear cells.
abstract: Formation of multinucleated giant cells (MGCs) by macrophage fusion is a typical cytopathic effect of lentiviral replication in caprine monocytes and MGC formation from cultured caprine peripheral blood mononuclear cells (PBMCs) has been considered to be diagnostic for small ruminant lentivirus (SRLV) infection. In this study, formation of MGCs was observed after 7-14 days when PBMCs were cultured from healthy goats free from SRLV infection. These MGCs expressed tartrate-resistant acid phosphatase, calcitonin receptor, integrin αVβ3, cathepsin K and matrix metalloproteinase 9 and were able to resorb bone in vitro in the absence of RANKL and macrophage colony stimulating factor, consistent with an osteoclast phenotype.
authors.affiliation: Università degli Studi di Milano, Dipartimento di Patologia Animale, Igiene e Sanità Pubblica Veterinaria, via Celoria 10, 20133 Milano, Italy. giuliano.pisoni@unimi.it
authors.forename: Giuliano
authors.initials: G
authors.lastname: Pisoni
authors.forename: Patrizia
authors.initials: P
authors.lastname: D'Amelio
authors.forename: Francesca
authors.initials: F
authors.lastname: Sassi
authors.forename: Giovanni
authors.initials: G
authors.lastname: Manarolla
authors.forename: Licia
authors.initials: L
authors.lastname: Scaccabarozzi
authors.forename: Clara
authors.initials: C
authors.lastname: Locatelli
authors.forename: Nausicaa
authors.initials: N
authors.lastname: Mazzocchi
authors.forename: Mirko
authors.initials: M
authors.lastname: Baruscotti
authors.forename: Dario
authors.initials: D
authors.lastname: DiFrancesco
authors.forename: Paolo
authors.initials: P
authors.lastname: Moroni
mesh_terms: D000135:Acid Phosphatase / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001842:Bone and Bones / Q000378:metabolism; D002478:Cells, Cultured; D005786:Gene Expression Regulation; D015726:Giant Cells / Q000473:pathology*; D006041:Goats; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D016173:Macrophage Colony-Stimulating Factor / Q000235:genetics / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D009000:Monocytes / Q000378:metabolism*; D010010:Osteoclasts / Q000166:cytology* / Q000378:metabolism / Q000473:pathology; D010447:Peptide Hydrolases / Q000235:genetics / Q000378:metabolism; D010641:Phenotype; D053245:RANK Ligand / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D000071681:Tartrate-Resistant Acid Phosphatase
chemical_list: D007527:Isoenzymes; D008565:Membrane Proteins; D053245:RANK Ligand; D016173:Macrophage Colony-Stimulating Factor; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase; D010447:Peptide Hydrolases