Efficient and cost-effective extraction of genomic DNA from formalin-fixed and paraffin-embedded tissues.
Diagnostic and investigative molecular pathology frequently has to resort to extraction of DNA from formalin-fixed and paraffin-embedded tissue samples. Although many different protocols are reported for this type of material, extraction of sufficient amounts of intact DNA is still challenging. Here, the authors report a reproducible, simple, cost-effective, and efficient protocol that yields up to 140 μg of DNA from approximately 10 to 15 mg of formalin-fixed and paraffin-embedded tissue samples and compare it to available protocols. The protocol allows stable amplification of DNA fragments up to 600 bp in length in a wide variety of tissues.
Weiss
A Th A
AT
Department of Veterinary Pathology, Freie Universität Berlin, Robert-von-Ostertag-Str. 15, 14163 Berlin, Germany. weiss.alexander@vetmed.fu-berlin.de
Delcour
N M
NM
Meyer
A
A
Klopfleisch
R
R
D000818:Animals; D002415:Cats / Q000235:genetics*; D003362:Cost-Benefit Analysis; D004247:DNA / Q000235:genetics / Q000302:isolation & purification*; D004285:Dogs / Q000235:genetics*; D004587:Electrophoresis, Agar Gel / Q000191:economics; D005404:Fixatives / Q000737:chemistry; D005557:Formaldehyde / Q000737:chemistry; D016678:Genome / Q000235:genetics; D016612:Paraffin Embedding / Q000191:economics / Q000662:veterinary*; D016133:Polymerase Chain Reaction / Q000191:economics; D016707:Tissue Fixation / Q000191:economics / Q000662:veterinary*
D005404:Fixatives; D005557:Formaldehyde; D004247:DNA