title: Expression of pro-inflammatory TACE-TNF-α-amphiregulin axis in Sjögren's syndrome salivary glands.
abstract: The tumor-necrosis-factor-converting-enzyme (TACE)-TNF-α-Amphiregulin (AREG) axis plays an important pathogenic role in inflammatory and autoimmune disorders. However, the pathological roles of these proteins in the chronic autoimmune disease Sjögren's syndrome (SS) remain to be elucidated. It is known that the TACE-AREG axis is clearly part of a larger cascade of signals that starts with the activation of Furin, responsible for maturation of TACE that, in turn, determines the production of active TNF-α, directly involved in the up-regulation of AREG expression. This study showed that Furin, TACE, TNF-α, and AREG proteins, detected in acinar and ductal cells of human salivary glands from SS patients, increased remarkably in comparison with biopsies of labial salivary glands from healthy controls. The changes in Furin, TACE, TNF- α, and AREG proteins' level detected in salivary glands biopsies of SS patients could be responsible for pro-inflammatory cytokines overexpression characterizing Sjögren's syndrome.
authors.affiliation: Department of Human Anatomy and Histology, Laboratory of Cell Biology, University of Bari Medical School, Piazza Giulio Cesare 1, 70124 Bari, Italy. m.sisto@anatomia.uniba.it
authors.forename: Margherita
authors.initials: M
authors.lastname: Sisto
authors.forename: Sabrina
authors.initials: S
authors.lastname: Lisi
authors.forename: Dario Domenico
authors.initials: DD
authors.lastname: Lofrumento
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Ingravallo
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Mitolo
authors.forename: Massimo
authors.initials: M
authors.lastname: D'Amore
mesh_terms: D051722:ADAM Proteins / Q000235:genetics* / Q000378:metabolism; D000072198:ADAM17 Protein; D066258:Amphiregulin; D001483:Base Sequence; D015153:Blotting, Western; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D018076:DNA, Complementary; D066255:EGF Family of Proteins; D045683:Furin / Q000235:genetics* / Q000378:metabolism; D006023:Glycoproteins / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D007249:Inflammation; D036341:Intercellular Signaling Peptides and Proteins / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012469:Salivary Glands / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D017421:Sequence Analysis; D012859:Sjogren's Syndrome / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism
chemical_list: C587848:AREG protein, human; D066258:Amphiregulin; D016207:Cytokines; D018076:DNA, Complementary; D066255:EGF Family of Proteins; D006023:Glycoproteins; D036341:Intercellular Signaling Peptides and Proteins; D014409:Tumor Necrosis Factor-alpha; D045683:Furin; D051722:ADAM Proteins; D000072198:ADAM17 Protein; C000606462:ADAM17 protein, human
references.citation: Nature. 1997 Feb 20;385(6618):729-33
references.pmid: 9034190
references.citation: Nature. 1997 Feb 20;385(6618):733-6
references.pmid: 9034191
references.citation: J Immunol. 1994 Jun 1;152(11):5532-9
references.pmid: 8189070
references.citation: Clin Exp Immunol. 1998 Dec;114(3):485-90
references.pmid: 9844061
references.citation: J Biol Chem. 2004 Jun 4;279(23):24179-88
references.pmid: 15066986
references.citation: J Invest Dermatol. 1998 Nov;111(5):715-21
references.pmid: 9804327
references.citation: Proc Natl Acad Sci U S A. 1993 Feb 1;90(3):863-7
references.pmid: 8430098
references.citation: J Cell Biol. 2004 Mar 1;164(5):769-79
references.pmid: 14993236
references.citation: Ann Rheum Dis. 2003 Apr;62(4):359-62
references.pmid: 12634239
references.citation: Reumatismo. 2007 Jul-Sep;59(3):221-6
references.pmid: 17898882
references.citation: Immunol Cell Biol. 2010 Mar-Apr;88(3):297-304
references.pmid: 19949420
references.citation: Int J Immunopathol Pharmacol. 2008 Oct-Dec;21(4):1045-7
references.pmid: 19144293
references.citation: Br J Rheumatol. 1998 Jul;37(7):779-83
references.pmid: 9714357
references.citation: Ann N Y Acad Sci. 2009 Aug;1171:407-14
references.pmid: 19723083
references.citation: Apoptosis. 2006 Dec;11(12):2205-15
references.pmid: 17051336
references.citation: Autoimmunity. 2004 Jun;37(4):305-7
references.pmid: 15518047
references.citation: Int Immunol. 2009 Dec;21(12):1341-9
references.pmid: 19854762
references.citation: J Mammary Gland Biol Neoplasia. 2008 Jun;13(2):181-94
references.pmid: 18470483
references.citation: J Inflamm (Lond). 2008 Apr 27;5:5
references.pmid: 18439312
references.citation: J Lab Clin Med. 2002 Jan;139(1):13-9
references.pmid: 11873240
references.citation: Immunobiology. 2010 Nov;215(11):874-83
references.pmid: 20061048
references.citation: Semin Arthritis Rheum. 2005 Apr;34(5 Suppl1):3-6
references.pmid: 15852247
references.citation: Coll Antropol. 2004;28 Suppl 2:305-9
references.pmid: 15571105
references.citation: J Biol Chem. 2002 Apr 12;277(15):12838-45
references.pmid: 11823465
references.citation: J Biol Chem. 2001 Dec 14;276(50):46722-8
references.pmid: 11592962
references.citation: FEBS Lett. 2003 Nov 20;554(3):275-83
references.pmid: 14623079
references.citation: J Biol Chem. 1998 Jul 3;273(27):17258-68
references.pmid: 9642297
references.citation: J Reprod Dev. 2010 Jun;56(3):315-23
references.pmid: 20168049
references.citation: Clin Exp Rheumatol. 2001 Mar-Apr;19(2):131-7
references.pmid: 11326474
references.citation: Proc Natl Acad Sci U S A. 1995 Mar 28;92(7):2840-4
references.pmid: 7708734
references.citation: Mol Pharmacol. 2001 Oct;60(4):646-55
references.pmid: 11562425
references.citation: Arch Dermatol Res. 2009 Jan;301(1):87-91
references.pmid: 18825399
references.citation: DNA Res. 2005;12(6):429-39
references.pmid: 16769699
references.citation: Ann Rheum Dis. 2002 Jun;61(6):554-8
references.pmid: 12006334
references.citation: Curr Dir Autoimmun. 2010;11:1-26
references.pmid: 20173385
references.citation: Lab Invest. 1985 May;52(5):559-67
references.pmid: 2581066
references.citation: J Inflamm (Lond). 2005 Nov 25;2:15
references.pmid: 16309552
references.citation: Autoimmunity. 1992;12(3):185-91
references.pmid: 1343766
references.citation: Proc Natl Acad Sci U S A. 1988 Sep;85(17):6528-32
references.pmid: 3413110
references.citation: Arch Oral Biol. 2002 Jun;47(6):443-8
references.pmid: 12102760
references.citation: Life Sci. 2006 May 22;78(26):3051-7
references.pmid: 16427093