title: Impact of long-term storage on stability of standard DNA for nucleic acid-based methods.
abstract: Real-time PCR is dependent upon a calibration function for quantification. While long-term storage of standards saves cost and time, solutions of DNA are prone to degradation. We present here the benchmark treatment for preservation of DNA standards, involving storage in 50% glycerol-double-distilled water, whereby a deviation of 0.2 threshold cycle (C(T)) values resulted after 100 days of storage.
authors.affiliation: Christian Doppler Laboratory for Molecular Food Analytics, Veterinärplatz 1, 1210 Vienna, Austria.
authors.forename: Barbara
authors.initials: B
authors.lastname: Röder
authors.forename: Karin
authors.initials: K
authors.lastname: Frühwirth
authors.forename: Claus
authors.initials: C
authors.lastname: Vogl
authors.forename: Martin
authors.initials: M
authors.lastname: Wagner
authors.forename: Peter
authors.initials: P
authors.lastname: Rossmanith
mesh_terms: D019411:Clinical Laboratory Techniques / Q000379:methods / Q000592:standards*; D015925:Cryopreservation / Q000379:methods; D004247:DNA / Q000032:analysis* / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods / Q000592:standards*; D011309:Preservation, Biological / Q000379:methods*; D012015:Reference Standards; D013048:Specimen Handling / Q000379:methods*
chemical_list: D004247:DNA
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