A novel ncRNA gene from mouse chromosome 5 trans-splices with Dmrt1 on chromosome 19.
Dmrt1 (Dsx- and Mab3-related transcription factor-1), a conserved transcription factor in different phyla, is a key regulator in sex determination. Here, we report the novel ncRNA gene Dmr (Dmrt1-related gene), from mouse chromosome 5 that trans-splices with Dmrt1 from chromosome 19 to generate a Dmrt1 protein that lacks the C-terminus. Dmr is mouse and rat specific, and the surrounding genes are also conserved in both species. Dmr is alternatively spliced, and three isoforms, Dmr a, b and c, are detected in the testis. Further, Dmr serves mainly as a 3' UTR, promotes trans-splicing and down-regulates the Dmrt1 protein. These results suggest that Dmr might play a negative regulatory role for Dmrt1 in male sexual development.
Zhang
Lei
L
Department of Genetics and Cell Biology, College of Life Science, Wuhan University, Wuhan 430072, China.
Lu
Heng
H
Xin
Dazhuan
D
Cheng
Hanhua
H
Zhou
Rongjia
R
D020413:3' Untranslated Regions / Q000235:genetics / Q000502:physiology*; D000818:Animals; D002478:Cells, Cultured; D033481:Chromosomes, Mammalian / Q000235:genetics*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D022661:RNA, Untranslated / Q000235:genetics / Q000502:physiology*; D051381:Rats; D012708:Sertoli Cells / Q000378:metabolism; D046468:Sexual Development / Q000235:genetics*; D020040:Trans-Splicing; D014157:Transcription Factors / Q000235:genetics*
D020413:3' Untranslated Regions; C110547:DMRT1 protein; D022661:RNA, Untranslated; D014157:Transcription Factors