title: HSPA1A is upregulated in periodontal ligament at early stage of tooth movement in rats.
abstract: Heat shock proteins (HSPs) are molecular chaperones that maintain intracellular protein homeostasis and ensure survival of cells. Continuous orthodontic force on the tooth is considered to be a type of physical stress loaded to the periodontal ligament (PDL). However, little is known about the role of HSPs during tooth movement. This study was performed to examine the expression of HSPs in the PDL during tooth movement using laser microdissection, microarray analysis, real-time RT-PCR and immunohistochemistry. Gene expression of HSPA1A in the pressure zone of the PDL was higher during 6 h of tooth movement than in the control group. Expression of HSPA1A decreased with time. HSPA1A was also detected in the pressure zone of the PDL at the protein level 24 h after the initial tissue change. These results strongly suggest that expression of HSPA1A in the PDL during early stages of tooth movement is a critical factor for tissue reaction.
authors.affiliation: Department of Orthodontics, Tsurumi University School of Dental Medicine, Tsurumi University, 2-1-3 Tsurumi, Tsurumi-ku, Yokohama, Kanagawa 230-8501, Japan. arai-chihiro@tsurumi-u.ac.jp
authors.forename: Chihiro
authors.initials: C
authors.lastname: Arai
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Nomura
authors.forename: Misao
authors.initials: M
authors.lastname: Ishikawa
authors.forename: Koji
authors.initials: K
authors.lastname: Noda
authors.forename: Jae-Won
authors.initials: JW
authors.lastname: Choi
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Yashiro
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Hanada
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Nakamura
mesh_terms: D000818:Animals; D016207:Cytokines / Q000378:metabolism; D003799:Dental Stress Analysis; D015870:Gene Expression; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics* / Q000378:metabolism*; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D007249:Inflammation / Q000378:metabolism; D046228:Microarray Analysis; D009967:Orthodontic Appliances; D010513:Periodontal Ligament / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013314:Stress, Mechanical; D014087:Tooth Movement Techniques
chemical_list: D016207:Cytokines; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins; C097210:Hspa1a protein, rat; D012333:RNA, Messenger
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