[Improving infection efficiency of adult cynomolgus monkey mesenchymal stem cells with lentiviral vectors].
Mesenchymal stem cells (MSCs) have received considerable attention for various therapeutic approaches in recent years. MSCs are also easy to genetically modify to express therapeutic genes by using lentiviral vectors. Because of the similarities in genetics, physiology and metabolism between non-human primates (NHPs) and humans, NHPs models are invaluable for researching human disorders and for developing therapeutic strategies. Therefore, MSCs derived from NHPs could be a powerful tool for cell therapy and genetic engineering. Studies from captive and free-ranging adult NHPs show that up to 100% were infected with simian foamy virus (SFV). In this study, we found that all cultured MSCs derived from adult cynomolgus monkey were infected with SFV by RT-PCR. Therefore, antiviral drugs must be added in MSCs culture. However, because of SFV infection and additive antiviral drugs, the infection efficiency of the lentiviral vectors reduced significantly. In this study, we improved the infection efficiency by disabled antiviral drugs before lentiviral infection. It might be provide technical assistance for the culture of adult cynomolgus monkey MSCs as genetically engineered cells applied to clinical and experimental research.
Ren
Zhenhua
Z
Cell Therapy Center, Xuanwu Hospital, Capital Medical University, Beijing 100053, China.
Wang
Shuyan
S
Zhang
Ying
Y
Zou
Chunlin
C
Zhang
Y Alex
YA
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D002454:Cell Differentiation; D005822:Genetic Vectors / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000235:genetics; D016086:Lentivirus / Q000235:genetics*; D008252:Macaca fascicularis; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000821:virology; D053150:Simian foamy virus / Q000502:physiology; D014161:Transduction, Genetic
D049452:Green Fluorescent Proteins