Osteogenic potential of mandibular vs. long-bone marrow stromal cells.
Although fundamentally similar to other bones, the jaws demonstrate discrete responses to developmental, mechanical, and homeostatic regulatory signals. Here, we hypothesized that rat mandible vs. long-bone marrow-derived cells possess different osteogenic potential. We established a protocol for rat mandible and long-bone marrow stromal cell (BMSC) isolation and culture. Mandible BMSC cultures formed more colonies, suggesting an increased CFU-F population. Both mandible and long-bone BMSCs differentiated into osteoblasts. However, mandible BMSCs demonstrated augmented alkaline phosphatase activity, mineralization, and osteoblast gene expression. Importantly, upon implantation into nude mice, mandible BMSCs formed 70% larger bone nodules containing three-fold more mineralized bone compared with long-bone BMSCs. Analysis of these data demonstrates an increased osteogenic potential and augmented capacity of mandible BMSCs to induce bone formation in vitro and in vivo. Our findings support differences in the mechanisms underlying mandible homeostasis and the pathophysiology of diseases unique to the jaws.
Aghaloo
T L
TL
Division of Diagnostic and Surgical Sciences, UCLA School of Dentistry, 10833 LeConte Ave., CHS Rm. 53-068, Los Angeles, CA 90095-1668, USA.
Chaichanasakul
T
T
Bezouglaia
O
O
Kang
B
B
Franco
R
R
Dry
S M
SM
Atti
E
E
Tetradis
S
S
D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D001854:Bone Marrow Cells / Q000502:physiology*; D016026:Bone Marrow Transplantation; D002113:Calcification, Physiologic / Q000502:physiology; D002356:Cartilage / Q000166:cytology; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology; D002469:Cell Separation; D016326:Extracellular Matrix Proteins / Q000032:analysis; D005781:Gelatin Sponge, Absorbable; D006706:Homeostasis / Q000502:physiology; D021621:Imaging, Three-Dimensional / Q000379:methods; D008334:Mandible / Q000166:cytology*; D051379:Mice; D008819:Mice, Nude; D010006:Osteoblasts / Q000502:physiology; D010011:Osteocytes / Q000166:cytology; D010012:Osteogenesis / Q000502:physiology*; D010750:Phosphoproteins / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D012795:Sialoglycoproteins / Q000032:analysis; D017154:Stromal Cells / Q000502:physiology* / Q000637:transplantation; D040521:Subcutaneous Tissue / Q000473:pathology / Q000601:surgery; D013977:Tibia / Q000166:cytology*; D054457:Tissue Scaffolds; D055114:X-Ray Microtomography / Q000379:methods; D000099311:Dentin Sialophosphoprotein
D000469:Alkaline Phosphatase; D016326:Extracellular Matrix Proteins; D010750:Phosphoproteins; D012795:Sialoglycoproteins; D000099311:Dentin Sialophosphoprotein
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