Detection of Mycobacterium avium subsp. paratuberculosis in drinking water and biofilms by quantitative PCR.
It has been suggested that Mycobacterium avium subspecies paratuberculosis has a role in Crohn's disease. The organism may be acquired but is difficult to culture from the environment. We describe a quantitative PCR (qPCR) method to detect M. avium subsp. paratuberculosis in drinking water and the results of its application to drinking water and faucet biofilm samples collected in the United States.
Beumer
Amy
A
University of Cincinnati, Raymond Walters College, Blue Ash, Ohio 45236, USA.
King
Dawn
D
Donohue
Maura
M
Mistry
Jatin
J
Covert
Terry
T
Pfaller
Stacy
S
D018441:Biofilms; D004269:DNA, Bacterial / Q000235:genetics; D016927:Mycobacterium avium subsp. paratuberculosis / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D014481:United States; D014871:Water Microbiology
D004269:DNA, Bacterial
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