Quantitative analysis of replication and tropisms of Dengue virus type 2 in Aedes albopictus.
Dengue virus serotype 2 (DENV-2) RNA replication profiles and tropisms were studied by using quantitative RT-PCR (q-RTPCR) in intrathoracically infected Aedes albopictus. The virus RNA replication profiles were diverse in mosquito organs. In fat body, brain, salivary gland, and malpighian tubes, it peaked at 8, 23, 23, and 27 days post-infection, respectively, and then, all declined. In midgut, it increased all the time and had no trend of decline. In ovary, it had no apparent increase. Subsequent Western blotting of DENV-2 E protein had similar results. Using ribosomal protein 7 (rpS7) as an internal control, we found that, in salivary gland, brain, fat body, and midgut, the average DENV-2 RNA levels (DENV-2 RNA/rpS7 mRNA) were 1,028, 464, 5.6, and 6.2, respectively; in malpighian tubes, it was 1, and in ovary, it was far less than 1. These results suggest that infection profiles and tropism of DENV-2 RNA in Ae. albopictus organs are significantly different.
Zhang
Meichun
M
Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, People's Republic of China. zhanxm@mail.sysu.edu.cn
Zheng
Xiaoying
X
Wu
Yu
Y
Gan
Ming
M
He
Ai
A
Li
Zhuoya
Z
Liu
Jing
J
Zhan
Ximei
X
D000330:Aedes / Q000821:virology*; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D003716:Dengue Virus / Q000502:physiology*; D014779:Virus Replication
D017931:DNA Primers
Am J Trop Med Hyg. 1979 Nov;28(6):1045-52
507282
Insect Biochem Mol Biol. 2009 Jan;39(1):47-54
18977438
Am J Trop Med Hyg. 1998 Dec;59(6):965-70
9886207
Jpn J Infect Dis. 2007 Sep;60(5):245-9
17881861
J Med Entomol. 1993 May;30(3):524-30
8510112
Am J Trop Med Hyg. 1992 Apr;46(4):489-501
1575297
N Engl J Med. 2005 Sep 1;353(9):924-32
16135837
Genetics. 2000 Oct;156(2):687-98
11014816
Am J Trop Med Hyg. 1976 Mar;25(2):318-25
1259091
BMC Microbiol. 2008 Mar 17;8:47
18366655
Insect Biochem Mol Biol. 2005 Nov;35(11):1293-307
16203210
J Med Entomol. 1979 Nov 7;16(4):343-9
396372
Am J Trop Med Hyg. 1987 Jan;36(1):143-52
3812879
Am J Trop Med Hyg. 1983 May;32(3):590-4
6859404
Am J Trop Med Hyg. 1988 Aug;39(2):218-22
3407842
Insect Mol Biol. 2004 Aug;13(4):387-98
15271211
Proc Natl Acad Sci U S A. 1997 Oct 14;94(21):11508-13
9326640
PLoS Negl Trop Dis. 2009;3(4):e408
19365540
Arch Virol Suppl. 2005;(19):89-100
16355869
J Biol Chem. 2006 Mar 31;281(13):8426-35
16449228
Am J Trop Med Hyg. 1987 Nov;37(3):640-7
3688318
Am J Trop Med Hyg. 2002 Jul;67(1):85-92
12363070
Insect Biochem Mol Biol. 2008 Mar;38(3):331-45
18252247
Am J Trop Med Hyg. 1983 Sep;32(5):1108-19
6625066
J Med Entomol. 2004 May;41(3):467-75
15185952
Arch Med Res. 2002 Jul-Aug;33(4):330-42
12234522
J Biol Chem. 2005 Apr 22;280(16):16499-507
15722339
Am J Trop Med Hyg. 2006 Jan;74(1):132-41
16407358
Acta Virol. 2001 Apr;45(2):67-71
11719984
BMC Microbiol. 2007 Jan 30;7:9
17263893
Am J Trop Med Hyg. 1990 Sep;43(3):308-13
2221225
Curr Opin Immunol. 2001 Feb;13(1):79-88
11154922
Med Vet Entomol. 2004 Sep;18(3):215-27
15347388
Am J Trop Med Hyg. 2004 Jan;70(1):89-97
14971704
Gene. 2000 Jun 13;251(1):9-17
10863091
PLoS Pathog. 2009 Feb;5(2):e1000299
19214215
Am J Trop Med Hyg. 2005 May;72(5):631-7
15891140
Am J Trop Med Hyg. 1977 Nov;26(6 Pt 1):1199-205
596516
Am J Trop Med Hyg. 1997 Aug;57(2):119-25
9288801
J Am Mosq Control Assoc. 1990 Jun;6(2):251-3
2370532
Vector Borne Zoonotic Dis. 2001 Summer;1(2):159-68
12680353
J Virol. 1988 Jun;62(6):2083-90
2896802
PLoS Pathog. 2008 Jul 04;4(7):e1000098
18604274
Am J Trop Med Hyg. 1998 Mar;58(3):283-6
9546404
J Am Mosq Control Assoc. 1990 Jun;6(2):312-4
2196337
J Med Entomol. 1977 Jan 31;13(4-5):469-72
845886
Am J Trop Med Hyg. 1984 Nov;33(6):1248-50
6542323
Lancet Infect Dis. 2002 Jan;2(1):33-42
11892494
J Med Entomol. 1992 Nov;29(6):985-9
1460640
Am J Trop Med Hyg. 2003 Feb;68(2):203-8
12641412
J Med Entomol. 1985 Sep 20;22(5):469-75
4045943
Med Vet Entomol. 1996 Jan;10(1):87-92
8834747
Mech Dev. 2006 Jul;123(7):530-47
16829058
Am J Trop Med Hyg. 2002 Sep;67(3):260-5
12408664
Am J Trop Med Hyg. 2002 Aug;67(2):158-61
12389940
Am J Trop Med Hyg. 2003 May;68(5):539-44
12812340
Am J Trop Med Hyg. 1974 Nov;23(6):1153-60
4429185
Am J Trop Med Hyg. 1999 Feb;60(2):292-9
10072154