[Effect of anti-glaucoma drugs on inflammatory cytokine production by human and murine peripheral blood mononuclear cells].
OBJECTIVE
We investigated both human and murine peripheral blood mononuclear cells to find which of the anti-glaucoma drugs used to lower intraocular pressure drug also suppresses inflammation.


METHODS
Peripheral blood mononuclear cells (PBMCs) prepared from healthy men and from BALB/c mice were suspended in RPMI 1640 culture medium containing 10% FBS. Each test drug was added to the cells, and they were cultured in a CO2 incubator (set to 37 degrees C and 5% CO2) for 30 minutes. Lipopolysaccharide (LPS) was added to the same cells and they were cultured again for a given period of time, after which either tumor necrosis factor-alpha (TNF-alpha) or interleukin-6 (IL-6) levels in the culture medium were determined by ELISA.


RESULTS
Carteolol inhibited production of both TNF-alpha and IL-6 from PBMCs. The other test agents had no inhibitory effect. The carbonic anhydrase inhibitor (CAI) dorzolamide, isopropyl unoprostone and latanoprost had almost no effect on the production of inflammatory cytokines.


CONCLUSIONS
We demonstrated that carteolol exhibits an inhibitory action on the production of inflammatory cytokines from PBMCs because of its intrinsic sympathomimetic action. We also showed that CAI and PG drugs had almost no effect on inflammatory cytokine production.
Kawai
Kenji
K
Department of Ophthalmology, Tokai University School of Medicine, Japan. k-kawai@is.icc.u-tokai.ac.jp
Ohashi
Hideki
H
Suzuki
Takahiro
T
Kitagaki
Hideki
H
Fujisawa
Shigeki
S
D000319:Adrenergic beta-Antagonists / Q000494:pharmacology*; D000328:Adult; D000818:Animals; D002354:Carteolol / Q000494:pharmacology*; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D005901:Glaucoma / Q000188:drug therapy; D006801:Humans; D015850:Interleukin-6 / Q000096:biosynthesis*; D007429:Intraocular Pressure / Q000187:drug effects*; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
D000319:Adrenergic beta-Antagonists; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D002354:Carteolol