title: Does radiotherapy increase oxidative stress? A study with nasopharyngeal cancer patients revealing anomalies in isoprostanes measurements.
abstract: This study aimed to examine if exposure to ionizing radiation during clinical radiotherapy (RT) causes increased oxidative damage. Seven patients with nasopharyngeal cancer (NPC) who underwent RT took part in this controlled-trial study. Blood and urine samples were obtained for F(2)-isoprostanes (F(2)-IsoPs) measurement. Urinary F(2)-IsoPs levels were elevated pre-treatment and remained high (but did not increase) during treatment, but decreased to the normal range after treatment. Plasma F(2)-IsoPs decreased significantly after the start of treatment before rising midway through treatment. Levels decreased significantly to below baseline following treatment. However, the patients were observed to have substantially lower levels of plasma esterified arachidonic acid (AA) residues than controls. The data shows that NPC is associated with elevated F(2)-isoprostanes in urine and in plasma after correction for decreased AA levels. RT did not increase these levels and, indeed, was associated with falls in F(2)-IsoPs. The validity and usefulness of correction of plasma F(2)-IsoPs for lowered AA levels is discussed.
authors.affiliation: Department of Radiation Oncology, National University Cancer Institute, Singapore.
authors.forename: Keith H C
authors.initials: KH
authors.lastname: Lim
authors.forename: Chung-Yung J
authors.initials: CY
authors.lastname: Lee
authors.forename: Arul
authors.initials: A
authors.lastname: Earnest
authors.forename: Raymond C S
authors.initials: RC
authors.lastname: Seet
authors.forename: Barry
authors.initials: B
authors.lastname: Halliwell
mesh_terms: D000328:Adult; D000368:Aged; D002277:Carcinoma / Q000097:blood / Q000532:radiotherapy* / Q000652:urine; D019583:Dose Fractionation, Radiation; D028441:F2-Isoprostanes / Q000032:analysis / Q000097:blood* / Q000652:urine*; D005500:Follow-Up Studies; D006801:Humans; D015994:Incidence; D008297:Male; D008875:Middle Aged; D009303:Nasopharyngeal Neoplasms / Q000097:blood / Q000532:radiotherapy* / Q000652:urine; D018384:Oxidative Stress / Q000528:radiation effects*; D011832:Radiation Injuries / Q000097:blood / Q000453:epidemiology / Q000652:urine; D011878:Radiotherapy / Q000009:adverse effects*; D015854:Up-Regulation / Q000528:radiation effects
chemical_list: D028441:F2-Isoprostanes