title: Parenteral antibiotics reduce bifidobacteria colonization and diversity in neonates.
abstract: We investigated the impact of parenteral antibiotic treatment in the early neonatal period on the evolution of bifidobacteria in the newborn. Nine babies treated with intravenous ampicillin/gentamicin in the first week of life and nine controls (no antibiotic treatment) were studied. Denaturing gradient gel electrophoresis was used to investigate the composition of Bifidobacterium in stool samples taken at four and eight weeks. Bifidobacteria were detected in all control infants at both four and eight weeks, while only six of nine antibiotic-treated infants had detectable bifidobacteria at four weeks and eight of nine at eight weeks. Moreover, stool samples of controls showed greater diversity of Bifidobacterium spp. compared with antibiotic-treated infants. In conclusion, short-term parenteral antibiotic treatment of neonates causes a disturbance in the expected colonization pattern of bifidobacteria in the first months of life. Further studies are required to probiotic determine if supplementation is necessary in this patient group.
authors.affiliation: Department of Paediatrics and Child Health, University College Cork, Cork, Ireland.
authors.forename: Séamus
authors.initials: S
authors.lastname: Hussey
authors.forename: Rebecca
authors.initials: R
authors.lastname: Wall
authors.forename: Emma
authors.initials: E
authors.lastname: Gruffman
authors.forename: Lisa
authors.initials: L
authors.lastname: O'Sullivan
authors.forename: C Anthony
authors.initials: CA
authors.lastname: Ryan
authors.forename: Brendan
authors.initials: B
authors.lastname: Murphy
authors.forename: Gerald
authors.initials: G
authors.lastname: Fitzgerald
authors.forename: Catherine
authors.initials: C
authors.lastname: Stanton
authors.forename: R Paul
authors.initials: RP
authors.lastname: Ross
references.citation: Nutr Clin Care. 2004 Apr-Jun;7(2):56-68
references.pmid: 15481739
references.citation: JAMA. 2008 Jul 2;300(1):60-70
references.pmid: 18594041
references.citation: Syst Appl Microbiol. 2002 Aug;25(2):249-58
references.pmid: 12353880
references.citation: Arch Dis Child Fetal Neonatal Ed. 1999 May;80(3):F167-73
references.pmid: 10212075
references.citation: Anaerobe. 2003 Oct;9(5):219-29
references.pmid: 16887708
references.citation: Dig Dis Sci. 2007 Sep;52(9):2069-77
references.pmid: 17420934
references.citation: Syst Appl Microbiol. 2002 Dec;25(4):536-43
references.pmid: 12583714
references.citation: J Allergy Clin Immunol. 2001 Oct;108(4):516-20
references.pmid: 11590374
references.citation: J Allergy Clin Immunol. 2003 Mar;111(3):587-91
references.pmid: 12642841
references.citation: Appl Environ Microbiol. 2001 Feb;67(2):504-13
references.pmid: 11157210
references.citation: Am J Clin Nutr. 1999 May;69(5):1035S-1045S
references.pmid: 10232646
references.citation: Pediatrics. 2006 Aug;118(2):511-21
references.pmid: 16882802
references.citation: Ir J Med Sci. 1992 Jan;161(1):3-4
references.pmid: 1506161
references.citation: Best Pract Res Clin Gastroenterol. 2003 Oct;17(5):725-40
references.pmid: 14507584
references.citation: Gene. 1987;57(2-3):267-72
references.pmid: 3319781
references.citation: J Allergy Clin Immunol. 2001 Jan;107(1):129-34
references.pmid: 11150002
references.citation: Am J Clin Nutr. 2008 Mar;87(3):534-8
references.pmid: 18326589
references.citation: Appl Environ Microbiol. 1999 Oct;65(10):4506-12
references.pmid: 10508082
references.citation: J Perinatol. 2005 Apr;25(4):265-9
references.pmid: 15616610
references.citation: J Pediatr Gastroenterol Nutr. 2000 Jan;30(1):61-7
references.pmid: 10630441
references.citation: Curr Opin Microbiol. 1999 Jun;2(3):317-22
references.pmid: 10383868
references.citation: Adv Pediatr. 2005;52:77-113
references.pmid: 16124337
references.citation: Clin Exp Allergy. 2007 Dec;37(12):1764-72
references.pmid: 17941914
references.citation: Br J Nutr. 2002 May;87(5):405-20
references.pmid: 12010580
references.citation: Appl Environ Microbiol. 1997 Apr;63(4):1268-73
references.pmid: 9097423
references.citation: Appl Environ Microbiol. 2003 Nov;69(11):6380-5
references.pmid: 14602589
references.citation: Cell Mol Life Sci. 2005 Jun;62(12):1339-48
references.pmid: 15971109
references.citation: J Microbiol Methods. 2005 Mar;60(3):365-73
references.pmid: 15649538
references.citation: Infection. 2002 Jun;30(3):158-60
references.pmid: 12120942