title: Surface modification on polyurethanes by using bioactive carboxymethylated fungal glucan from Poria cocos.
abstract: In this work, a water-insoluble β-D-glucan (PCSG), isolated from Poria cocos, was carboxymethylated to create a water-soluble derivative named as CP. After free amino groups have been introduced, CP was covalently immobilized onto PU surface. The hydrophilicity and the concentration of carboxyl group on the modified PU surface were determined. The fibrinogen and albumin adsorption to the surface, in vitro blood compatibility, and antibacterial activity of the surface against Pseudomonas aeruginosa were evaluated. The water contact angle measurement indicated that the hydrophilicity of PU surface increased after modification. The fibrinogen adsorption of the modified PU surface decreased 51.5%, compared with control PU. CP immobilization could prolong the blood coagulation time was suggested by APTT experiment. Antibacterial activity experiments indicated that CP modified surface obviously suppressed the growth of P. aeruginosa. Thereby, CP immobilization improves blood compatibility of PU surface and introduces special antibacterial bioactivity.
authors.affiliation: School of Materials Science and Engineering, Wuhan University of Technology, 122 Luoshi Road, Wuhan 430070, PR China. yifengwang@whut.edu.cn
authors.forename: Yifeng
authors.initials: Y
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Xu
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000327:Adsorption; D000900:Anti-Bacterial Agents / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D001777:Blood Coagulation / Q000187:drug effects; D001780:Blood Coagulation Tests; D005340:Fibrinogen / Q000737:chemistry / Q000378:metabolism; D005936:Glucans / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008826:Microbial Sensitivity Tests; D010316:Particle Size; D011140:Polyurethanes / Q000737:chemistry*; D055441:Poria / Q000737:chemistry*; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D012995:Solubility; D013499:Surface Properties; D014867:Water / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D005936:Glucans; D011140:Polyurethanes; D014867:Water; D005340:Fibrinogen