title: Comparison of two methods for blood lead analysis in cattle: graphite-furnace atomic absorption spectrometry and LeadCare(R) II system.
abstract: The current study compared the LeadCare(R) II test kit system with graphite-furnace atomic absorption spectrometry for blood lead (Pb) analysis in 56 cattle accidentally exposed to Pb in the field. Blood Pb concentrations were determined by LeadCare II within 4 hr of collection and after 72 hr of refrigeration. Blood Pb concentrations were determined by atomic absorption spectrometry, and samples that were coagulated (n = 12) were homogenized before analysis. There was strong rank correlation (R(2) = 0.96) between atomic absorption and LeadCare II (within 4 hr of collection), and a conversion formula was determined for values within the observed range (3-91 mcg/dl, although few had values >40 mcg/dl). Median and mean blood pb concentrations for atomic absorption were 7.7 and 15.9 mcg/dl, respectively; for LeadCare II, medians were 5.2 mcg/dl at 4 hr and 4.9 mcg/dl at 72 hr, and means were 12.4 and 11.7, respectively. LeadCare II results at 4 hr strongly correlated with 72 hr results (R(2) = 0.96), but results at 72 hr were lower (P < 0.01). There was no significant difference between coagulated and uncoagulated samples run by atomic absorption. Although there have been several articles that compared LeadCare with other analytical techniques, all were for the original system, not LeadCare II. The present study indicated that LeadCare II results correlated well with atomic absorption over a wide range of blood Pb concentrations and that refrigerating samples for up to 72 hr before LeadCare II analysis was acceptable for clinical purposes.
authors.affiliation: New York State Animal Health Diagnostic Center, Cornell University, Population Medicines and Sciences Health and Diagnostic Services, Ithaca, NY 14853, USA. klb72@cornell.edu
authors.forename: Karyn
authors.initials: K
authors.lastname: Bischoff
authors.forename: Cynthia
authors.initials: C
authors.lastname: Gaskill
authors.forename: Hollis N
authors.initials: HN
authors.lastname: Erb
authors.forename: Joseph G
authors.initials: JG
authors.lastname: Ebel
authors.forename: Joseph
authors.initials: J
authors.lastname: Hillebrandt
mesh_terms: D000818:Animals; D001777:Blood Coagulation; D002417:Cattle / Q000097:blood*; D002418:Cattle Diseases / Q000097:blood; D005069:Evaluation Studies as Topic; D006108:Graphite; D007854:Lead / Q000097:blood*; D007855:Lead Poisoning / Q000097:blood / Q000662:veterinary; D013054:Spectrophotometry, Atomic / Q000379:methods / Q000662:veterinary
chemical_list: D007854:Lead; D006108:Graphite