title: Crystallization and preliminary crystallographic characterization of the PAS domains of EAG and ELK potassium channels.
abstract: Per-Arnt-Sim (PAS) domains are ubiquitous in nature; they are approximately 130-amino-acid protein domains that adopt a fairly conserved three-dimensional structure despite their low degree of sequence homology. These domains constitute the N-terminus or, less frequently, the C-terminus of a number of proteins, where they exert regulatory functions. PAS-containing proteins generally display two or more copies of this motif. In this work, the crystallization and preliminary analysis of the PAS domains of two eukaryotic potassium channels from the ether-à-go-go (EAG) family are reported.
authors.affiliation: IBMC-Instituto de Biologia Molecular e Celular, Rua do Campo Alegre 823, Porto 4150-180, Portugal.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Adaixo
authors.forename: João Henrique
authors.initials: JH
authors.lastname: Morais-Cabral
mesh_terms: D000818:Animals; D003460:Crystallization; D018360:Crystallography, X-Ray; D029721:Drosophila Proteins / Q000737:chemistry*; D004331:Drosophila melanogaster / Q000737:chemistry*; D051638:Ether-A-Go-Go Potassium Channels / Q000737:chemistry*; D051379:Mice; D015221:Potassium Channels / Q000737:chemistry*; D017434:Protein Structure, Tertiary
chemical_list: D029721:Drosophila Proteins; C555038:Elk protein, Drosophila; D051638:Ether-A-Go-Go Potassium Channels; D015221:Potassium Channels
references.citation: Microbiol Mol Biol Rev. 1999 Jun;63(2):479-506
references.pmid: 10357859
references.citation: Acta Crystallogr D Biol Crystallogr. 1997 May 1;53(Pt 3):240-55
references.pmid: 15299926
references.citation: Cell. 1998 Nov 25;95(5):649-55
references.pmid: 9845367
references.citation: Acta Crystallogr D Biol Crystallogr. 2006 Jan;62(Pt 1):48-57
references.pmid: 16369093
references.citation: Biophys J. 2003 Apr;84(4):2474-82
references.pmid: 12668455
references.citation: Annu Rev Physiol. 2010;72:625-45
references.pmid: 20148691
references.citation: J Appl Crystallogr. 2007 Aug 1;40(Pt 4):658-674
references.pmid: 19461840
references.citation: Nat Struct Mol Biol. 2007 Aug;14(8):779-84
references.pmid: 17632523
references.citation: Protein Expr Purif. 2005 May;41(1):207-34
references.pmid: 15915565
references.citation: J Membr Biol. 2001 Jul 1;182(1):1-15
references.pmid: 11426295