Wheat germ cell-free protein production system for post-genomic research.
Genomic information becomes useful knowledge only when the structures and functions of gene products are understood. In spite of a vast array of analytical tools developed for biological studies in recent years, producing proteins at will is still a bottleneck in post-genomic studies. The cell-free protein production system we developed using wheat embryos has enabled us to produce high quality proteins for genome-wide functional and structural analyses and at the same time circumvent almost all the limitations, such as biohazards and costs, that have hampered conventional cell-free protein synthesis systems. In the present article, we introduce examples of our new wheat germ cell-free protein production system and its application to functional and structural analyses, with the focus on the former.
Madono
Masaki
M
CellFree Sciences Co., Ltd., 75-1, Ono-cho, Leading Venture Plaza 201, Tsurumi-ku, Yokohama 230-0046, Japan.
Sawasaki
Tatsuya
T
Morishita
Ryo
R
Endo
Yaeta
Y
D002474:Cell-Free System / Q000378:metabolism*; D057166:High-Throughput Screening Assays; D006801:Humans; D010447:Peptide Hydrolases / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D011487:Protein Conformation; D014908:Triticum / Q000196:embryology*
D010940:Plant Proteins; D010447:Peptide Hydrolases