title: Overexpression of LYRM1 induces mitochondrial impairment in 3T3-L1 adipocytes.
abstract: Homo sapiens LYR motif containing 1 (LYRM1) is a recently discovered gene involved in adipose tissue homeostasis and obesity-associated insulin resistance. The exact mechanism by which LYRM1 induces insulin resistance has not yet been fully elucidated. In this study, we demonstrated that the overexpression of LYRM1 in 3T3-L1 adipocytes resulted in reduced insulin-stimulated glucose uptake, an abnormal mitochondrial morphology, and a decrease in intracellular ATP synthesis and mitochondrial membrane potential. In addition, LYRM1 overexpression led to excessive production of intracellular of reactive oxygen species. Collectively, our results indicated that the overexpression of LYRM1 caused mitochondrial dysfunction in adipocytes, which might be responsible for the development of LYRM1-induced insulin resistance.
authors.affiliation: Nanjing Maternal and Child Health Hospital of Nanjing Medical University, Nanjing 210004, China.
authors.forename: Xin-Guo
authors.initials: XG
authors.lastname: Cao
authors.forename: Chun-Zhao
authors.initials: CZ
authors.lastname: Kou
authors.forename: Ya-Ping
authors.initials: YP
authors.lastname: Zhao
authors.forename: Chun-Lin
authors.initials: CL
authors.lastname: Gao
authors.forename: Chun
authors.initials: C
authors.lastname: Zhu
authors.forename: Chun-Mei
authors.initials: CM
authors.lastname: Zhang
authors.forename: Chen-Bo
authors.initials: CB
authors.lastname: Ji
authors.forename: Da-Ni
authors.initials: DN
authors.lastname: Qin
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.forename: Xi-Rong
authors.initials: XR
authors.lastname: Guo
mesh_terms: D041721:3T3-L1 Cells; D000255:Adenosine Triphosphate / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D000818:Animals; D051017:Apoptosis Regulatory Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D002478:Cells, Cultured; D004272:DNA, Mitochondrial / Q000235:genetics / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006706:Homeostasis / Q000187:drug effects; D007328:Insulin / Q000378:metabolism / Q000494:pharmacology; D007333:Insulin Resistance; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D051379:Mice; D008928:Mitochondria / Q000378:metabolism* / Q000473:pathology; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D051017:Apoptosis Regulatory Proteins; D004272:DNA, Mitochondrial; D007328:Insulin; C538836:LYRM1 protein, human; D017382:Reactive Oxygen Species; D000255:Adenosine Triphosphate; D005947:Glucose