Detection of early and single infections of Schistosoma japonicum in the intermediate host snail, Oncomelania hupensis, by PCR and loop-mediated isothermal amplification (LAMP) assay.
Polymerase chain reaction (PCR) with the specific primer set amplifying 28S ribosomal DNA (rDNA) of Schistosoma japonicum was able to detect genomic DNA of S. japonicum, but not S. mansoni, at 100 fg. This procedure enabled us to detect the DNA from a single miracidium and a snail infected with one miracidium at just 1 day after infection. We compared these results with those from loop-mediated isothermal amplification (LAMP) targeting 28S rDNA and found similar results. The LAMP could amplify the specific DNA from a group of 100 normal snails mixed with one infected snail A PCR screening of infected snails from endemic regions in Anhui Province revealed schistosomal DNA even in snails found negative by microscopy. PCR and LAMP show promise for monitoring the early infection rate in snails, and they may be useful for predicting the risk of infection in the endemic places.
Kumagai
Takashi
T
Section of Environmental Parasitology, Department of International Health Development, Division of Public Health, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan. tkuma.vip@tmd.ac.jp
Furushima-Shimogawara
Rieko
R
Ohmae
Hiroshi
H
Wang
Tian-Ping
TP
Lu
Shaohong
S
Chen
Rui
R
Wen
Liyong
L
Ohta
Nobuo
N
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D004275:DNA, Ribosomal / Q000235:genetics; D051379:Mice; D008813:Mice, Inbred ICR; D016133:Polymerase Chain Reaction / Q000379:methods*; D012549:Schistosoma japonicum / Q000235:genetics / Q000302:isolation & purification*; D012908:Snails / Q000469:parasitology*
D017931:DNA Primers; D004275:DNA, Ribosomal
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