P2X2/3 and P2X3 receptors contribute to the metaboreceptor component of the exercise pressor reflex.
The exercise pressor reflex is due to activation of thin fiber afferents within contracting muscle. These afferents are in part stimulated by ATP activation of purinergic 2X (P2X) receptors during contraction. Which of the P2X receptors contribute to the reflex is unknown; however, P2X2/3 and P2X3 receptor subtypes are good candidates because they are located on thin fiber afferents and are involved in sensory neurotransmission. To determine if P2X2/3 and P2X3 receptors evoke the metabolic component of the exercise pressor reflex, we examined the effect of two P2X2/3 and P2X3 antagonists, A-317491 (10 mg/kg) and RO-3 (10 mg/kg), on the pressor response to injections of α,β-methylene ATP (α,β-MeATP; 50 μg/kg), freely perfused static contraction, contraction of the triceps surae muscles while the circulation was occluded, and postcontraction circulatory occlusion in decerebrate cats. We found that the antagonists reduced the pressor response to α,β-MeATP injection (before Δ 20 ± 3 mmHg; drug Δ 11 ± 3 mmHg; P < 0.05), suggesting the antagonists were effective in blocking P2X2/3 and P2X3 receptors. P2X2/3 and P2X3 receptor blockade reduced the pressor response to freely perfused contraction (before Δ 33 ± 5 mmHg; drug Δ 15 ± 5 mmHg; P < 0.05), contraction with the circulation occluded (before Δ 52 ± 7 mmHg; drug Δ 20 ± 4 mmHg; P < 0.05), and during postcontraction circulatory occlusion (before Δ 15 ± 1 mmHg; drug Δ 5 ± 1 mmHg; P < 0.05). Our findings suggest that P2X2/3 and P2X3 receptors contribute to the metabolic component of the exercise pressor reflex in decerebrate cats.
McCord
Jennifer L
JL
Penn State Heart and Vascular Institute, Pennsylvania State University College of Medicine, Hershey, PA 17033, USA. jmccord@hmc.psu.edu
Tsuchimochi
Hirotsugu
H
Kaufman
Marc P
MP
D000255:Adenosine Triphosphate / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000378:metabolism*; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D002415:Cats; D003655:Decerebrate State; D004558:Electric Stimulation; D005260:Female; D009119:Muscle Contraction; D018482:Muscle, Skeletal / Q000294:innervation*; D009475:Neurons, Afferent / Q000378:metabolism*; D010636:Phenols / Q000008:administration & dosage; D005082:Physical Exertion; D011083:Polycyclic Compounds / Q000008:administration & dosage; D058920:Purinergic P2X Receptor Antagonists / Q000008:administration & dosage; D058476:Receptors, Purinergic P2X2 / Q000187:drug effects / Q000378:metabolism*; D058477:Receptors, Purinergic P2X3 / Q000187:drug effects / Q000378:metabolism*; D012018:Reflex / Q000187:drug effects; D013997:Time Factors
C470346:A-317491; D010636:Phenols; D011083:Polycyclic Compounds; D058920:Purinergic P2X Receptor Antagonists; D058476:Receptors, Purinergic P2X2; D058477:Receptors, Purinergic P2X3; D000255:Adenosine Triphosphate; C002630:alpha,beta-methyleneadenosine 5'-triphosphate
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