[Expression of uncoupling protein 2 on macrophages and its relation to interleukin-10/interferon ratio in patients with unexplained recurrent spontaneous abortion].
OBJECTIVE
To investigate the relationship between the expression of uncoupling protein 2 (UCP2) and ratio of interleukin-10/interferon-gamma (IL10/IFNgamma) in the macrophages from patients with unexplained recurrent spontaneous abortion (URSA).


METHODS
Twelve women undergoing selective termination of normal early pregnancy (control) and 11 having URSA were included in this study. Magnetic cell sorting (MACS) was used to isolate the macrophages in the decidua, and the expression of UCP2 was detected with flow cytometry. Cytokine (IL10 and IFNgamma) secretion by the macrophages was detected by enzyme-linked immunosorbent spot-forming (ELISPOT) cell assay.


RESULTS
Compared to the control group, the women with URSA showed significantly decreased expression of UCP2 on decidual macrophage (136-/+25 vs 201-/+31, P<0.01), and the expression of UCP2 was positively correlated to the ratio of IL10/IFNgamma(r=0.73, P<0.01).


CONCLUSIONS
UCP2 may play an important role in the regulation of macrophage activity and cytokine secretion to contribute to spontaneous abortion.
Fu
Zhi-hong
ZH
Department of Reproductive Healthcare, Shenzhen Maternity and Child Healthcare Hospital, Shenzhen 518000, China. fzhlxylxw@yahoo.com.cn
Zhu
Wen-jie
WJ
Chen
Xiu-min
XM
Li
Xue-mei
XM
Tan
Zhen
Z
Zhou
Yong-hong
YH
D000026:Abortion, Habitual / Q000378:metabolism*; D000328:Adult; D016207:Cytokines / Q000378:metabolism; D003656:Decidua / Q000166:cytology / Q000378:metabolism*; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D016753:Interleukin-10 / Q000378:metabolism; D007473:Ion Channels / Q000378:metabolism*; D008262:Macrophage Activation; D024101:Mitochondrial Proteins / Q000378:metabolism*; D011247:Pregnancy; D000071246:Uncoupling Protein 2
D016207:Cytokines; C508609:IL10 protein, human; D007473:Ion Channels; D024101:Mitochondrial Proteins; C000604389:UCP2 protein, human; D000071246:Uncoupling Protein 2; D016753:Interleukin-10; D007371:Interferon-gamma