An LC-MS method for simultaneous determination of nine ginsenosides in rat plasma and its application in pharmacokinetic study.
A sensitive and reliable LC-ESI-MS method for simultaneous determination of nine ginsenosides (Rh(1), Rg(2), Rg(1), Rf, Re, Rd, Rc, Rb(2) and Rb(1)) in rat plasma was developed and validated using saikosaponin A as an internal standard. The samples were extracted by solid-phase extraction. Chromatographic separation was carried out on a Hypersil Gold C(18) column (100 × 2.1 mm, 5 µm) by stepwise gradient elution with water (0.1% formic acid, v/v) and acetonitrile as the mobile phase. Detection was determined by selective ion monitoring mode using electrospray ionization in the negative ion mode. Good linearity over the investigated concentration ranges was observed with the values of r higher than 0.9900. The intra- and inter-day precisions were all no more than 15% and the average recoveries varied from 71.8 to 91.7%. This quantitative measurement was successfully applied to a pharmacokinetic study of Yi-Qi-Fu-Mai injection.
Zhou
Dandan
D
Key Laboratory of Standardization of Chinese Medicines of Ministry of Education, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, People's Republic of China.
Tong
Ling
L
Wan
Meixu
M
Wang
Gangli
G
Ye
Zhengliang
Z
Wang
Zhengtao
Z
Lin
Ruichao
R
D000818:Animals; D019540:Area Under Curve; D002853:Chromatography, Liquid / Q000379:methods*; D004355:Drug Stability; D004365:Drugs, Chinese Herbal / Q000032:analysis; D036145:Ginsenosides / Q000097:blood* / Q000493:pharmacokinetics; D016014:Linear Models; D008297:Male; D009828:Oleanolic Acid / Q000031:analogs & derivatives / Q000097:blood; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012503:Saponins / Q000097:blood; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D004365:Drugs, Chinese Herbal; D036145:Ginsenosides; D012503:Saponins; D009828:Oleanolic Acid; C025759:saikosaponin D