Metabonomic study on women of reproductive age treated with nutritional intervention: screening potential biomarkers related to neural tube defects occurrence.
Nutritional intervention is effective in reducing the risk of neural tube defects (NTDs). To determine the effects of nutritional supplementation on human metabolism, a metabonomic study was carried out on 96 women of reproductive age. Subjects with nutritional intervention were given fortified wheat flour (containing folic acid, vitamin B₁, vitamin B₂, ferric sodium edetate and zinc oxide) for 8 months. Serum metabolic fingerprinting was detected via ultraperformance liquid chromatography in tandem with time-of-flight mass spectrometry (UPLC-TOF MS), and data acquired was processed by multivariate statistical analysis. The result revealed a significant difference between the control and intervention group. Twenty potential biomarkers, including fructose 6-phosphate, sphingosine 1-phosphate, docosahexaenoic acid and hexadecanoic acid, were located and identified by the accurate mass measurement of TOF MS. These compounds are believed to be functionally related to anti-oxidative competence in vivo. In conclusion, metabonomics study is a valuable approach in exploring the effect mechanism of nutritional intervention on NTD prevention.
Jiang
Zhiting
Z
School of Pharmacy, East-China University of Science and Technology, Shanghai 200237, People's Republic of China.
Liang
Qionglin
Q
Wang
Yong
Y
Zheng
Xiaoying
X
Pei
Lijun
L
Zhang
Ting
T
Wang
Yiming
Y
Luo
Guoan
G
D000328:Adult; D015415:Biomarkers / Q000097:blood; D019587:Dietary Supplements; D005227:Fatty Acids / Q000097:blood; D005260:Female; D005433:Flour; D005527:Food, Fortified; D005636:Fructosephosphates / Q000097:blood; D006801:Humans; D016018:Least-Squares Analysis; D008246:Lysophospholipids / Q000097:blood; D055442:Metabolome; D055432:Metabolomics / Q000379:methods*; D015999:Multivariate Analysis; D009436:Neural Tube Defects / Q000378:metabolism* / Q000517:prevention & control; D015203:Reproducibility of Results; D013110:Sphingosine / Q000031:analogs & derivatives / Q000097:blood
D015415:Biomarkers; D005227:Fatty Acids; D005636:Fructosephosphates; D008246:Lysophospholipids; C060506:sphingosine 1-phosphate; C027618:fructose-6-phosphate; D013110:Sphingosine