Comparing cooling systems for the COBE 2991 cell separator used in the purification of human pancreatic islets of Langerhans.
Two different approaches of controlled cooling of the COBE 2991 cell-separator for islet purification were evaluated. The first method is the new Geneva COBE cooling system (GCCS), which consists of an electronically controlled liquid nitrogen injection system. The second is the University of Illinois at Chicago cooling system (UICCS), which consists of a specially designed "Cold Room" maintained at 1-8 C. For the GCCS, the mean temperatures of the gradient solutions were measured at the beginning and end of centrifugation were found to be 7 +/-0.7 C and 6.8 +/-0.6 C respectively. For the UICCS, the mean temperature of the gradients at the beginning and end of centrifugation were 4.7 +/-0.53 C and 7.03 C+/-0.91 C respectively. The presented COBE cooling systems can easily be adapted to a COBE 2991 cell-separator and are efficient in maintaining gradient solutions at a defined low temperature during centrifugation.
Adewola
A
A
Department of Transplant/Surgery, University of Illinois, Chicago, IL, USA.
Mage
R
R
Hansen
M
M
Barbaro
B
B
Mendoza-Elias
J
J
Harvat
T
T
Morel
P H
PH
Oberholzer
J
J
Wang
Y
Y
D002469:Cell Separation / Q000295:instrumentation* / Q000592:standards; D002498:Centrifugation / Q000295:instrumentation* / Q000592:standards; D004867:Equipment Design; D006801:Humans; D007515:Islets of Langerhans / Q000166:cytology*; D009584:Nitrogen; D012034:Refrigeration / Q000295:instrumentation* / Q000592:standards; D015203:Reproducibility of Results
D009584:Nitrogen