Organellar protein complexes of Caco-2 human cells analyzed by two-dimensional blue native/SDS-PAGE and mass spectrometry.
The complexome is essential for a better understanding of protein functions. In order to study protein complexes, an approach allowing the extraction and the analysis in native conditions is needed. Two-dimensional blue native/SDS-PAGE (2D BN/SDS-PAGE) technology is thus an interesting and powerful approach for this purpose. This report deals with the analysis and the identification of the organellar protein complexes of Caco-2 human cells using 2D BN/SDS-PAGE and HPLC-chip-MS. We identified 58 protein complexes (26 heteromultimeric and 32 homomultimeric complexes) and 4 monomeric proteins. Among them, 32 protein complexes were pointed out, providing insights into the function of previously uncharacterized human proteins.
Lasserre
Jean-Paul
JP
Laboratoire de Biochimie des Protéines et Protéomique, Université Paris 13, UMR CNRS 7033, 74 rue Marcel Cachin, Bobigny cedex, France. lasserre-jean-paul@orange.fr
Sylvius
Loïk
L
Joubert-Caron
Raymonde
R
Caron
Michel
M
Hardouin
Julie
J
D018938:Caco-2 Cells; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D046912:Multiprotein Complexes / Q000032:analysis*; D015388:Organelles / Q000378:metabolism; D011485:Protein Binding; D055503:Protein Multimerization; D020543:Proteome / Q000032:analysis / Q000737:chemistry; D040901:Proteomics / Q000379:methods*
D046912:Multiprotein Complexes; D020543:Proteome