VIVID interacts with the WHITE COLLAR complex and FREQUENCY-interacting RNA helicase to alter light and clock responses in Neurospora.
The photoreceptor and PAS/LOV protein VIVID (VVD) modulates blue-light signaling and influences light and temperature responses of the circadian clock in Neurospora crassa. One of the main actions of VVD on the circadian clock is to influence circadian clock phase by regulating levels of the transcripts encoded by the central clock gene frequency (frq). How this regulation is achieved is unknown. Here we show that VVD interacts with complexes central for circadian clock and blue-light signaling, namely the WHITE-COLLAR complex (WCC) and FREQUENCY-interacting RNA helicase (FRH), a component that complexes with FRQ to mediate negative feedback control in Neurospora. VVD interacts with FRH in the absence of WCC and FRQ but does not seem to control the exosome-mediated negative feedback loop. Instead, VVD acts to modulate the transcriptional activity of the WCC.
Hunt
Suzanne M
SM
Faculty of Life Sciences, University of Manchester, Manchester M13 9PT, United Kingdom.
Thompson
Seona
S
Elvin
Mark
M
Heintzen
Christian
C
D001483:Base Sequence; D002467:Cell Nucleus / Q000378:metabolism; D002940:Circadian Rhythm / Q000235:genetics / Q000502:physiology*; D003593:Cytoplasm / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000502:physiology*; D025461:Feedback, Physiological; D005656:Fungal Proteins / Q000235:genetics / Q000502:physiology*; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D008027:Light; D008954:Models, Biological; D009492:Neurospora crassa / Q000235:genetics / Q000502:physiology* / Q000528:radiation effects*; D020283:Photoreceptors, Microbial / Q000235:genetics / Q000502:physiology; D020365:RNA Helicases / Q000235:genetics / Q000502:physiology*; D012331:RNA, Fungal / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology*
D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D020283:Photoreceptors, Microbial; D012331:RNA, Fungal; D012333:RNA, Messenger; D014157:Transcription Factors; C423283:VVD protein, Neurospora crassa; C105589:wc-1 protein, Neurospora crassa; C103772:white collar 2 protein, Neurospora; D020365:RNA Helicases
Cell. 2005 Jul 29;122(2):235-46
16051148
Proc Natl Acad Sci U S A. 2003 May 13;100(10):5914-9
12714686
Fungal Genet Biol. 2004 Oct;41(10):897-910
15341912
Biochemistry. 2008 Jul 8;47(27):7012-9
18553928
EMBO J. 2003 Sep 15;22(18):4846-55
12970196
PLoS Biol. 2005 Apr;3(4):e95
15760278
Fungal Genet Biol. 2001 Apr;32(3):169-81
11343403
Genes Dev. 2005 Nov 1;19(21):2593-605
16264193
Cell. 2001 Feb 9;104(3):453-64
11239402
J Am Chem Soc. 2008 Sep 17;130(37):12226-7
18715002
Annu Rev Genet. 1996;30:579-601
8982466
J Mol Evol. 2003;57 Suppl 1:S286-9
15008426
Eur J Biochem. 2000 Jan;267(2):414-22
10632711
J Biol Chem. 2005 Apr 29;280(17):17526-32
15731099
EMBO J. 1996 Apr 1;15(7):1650-7
8612589
Science. 2007 May 18;316(5827):1054-7
17510367
Cell. 2009 Sep 18;138(6):1236-46
19747717
Science. 1997 May 2;276(5313):763-9
9115195
Eukaryot Cell. 2003 Apr;2(2):341-50
12684383
Genetics. 2010 Feb;184(2):351-61
19948888
Chronobiol Int. 2003 Sep;20(5):741-74
14535352
Genes Dev. 2007 Aug 1;21(15):1964-74
17671094
Nat Rev Genet. 2005 Jul;6(7):544-56
15951747
EMBO J. 2009 Apr 22;28(8):1029-42
19262566
Science. 2002 Aug 2;297(5582):815-9
12098706
Genes Dev. 2005 Dec 1;19(23):2888-99
16287715
Genes Dev. 2005 Jan 15;19(2):234-41
15625191
Science. 2002 Aug 2;297(5582):840-3
12098705
Proc Natl Acad Sci U S A. 2003 May 13;100(10):5938-43
12719523
EMBO J. 2001 Jan 15;20(1-2):109-17
11226161
Cell. 1995 Jun 30;81(7):1003-12
7600569
Mol Microbiol. 2001 Feb;39(4):1080-7
11251826
Biochem Soc Trans. 2005 Nov;33(Pt 5):949-52
16246018
Adv Genet. 2007;58:25-66
17452245
Science. 1994 Mar 18;263(5153):1578-84
8128244
Cold Spring Harb Symp Quant Biol. 2007;72:57-68
18522516