title: Comparative proteomic analyses of Streptococcus suis serotype 2 cell wall-associated proteins.
abstract: Streptococcus suis serotype 2 (SS2) is a zoonotic pathogen that is distributed throughout the world. Virulence factors and/or markers of the virulent serotype 2 strains have not been fully identified. In this study a simple, rapid, and non-destructive method was used to extract cell wall-associated proteins from SS2 strains. Two virulent strains were compared with one avirulent strain by 2-dimensional electrophoresis (2DE). When the results of the 2DE analyses were combined with the results of mass spectrometry analyses, a total of 40 unique proteins were identified, including 26 antigens (2DE immunoblotting was used as a preliminary study). In addition to a known virulence factor, muramidase-released protein, two new proteins, catabolite control protein A and leucyl aminopeptidase, and nine potential virulence factors were also identified. The formers may be a potential virulence regulator or drug target, and the latter contains plasminogen-binding proteins and molecular chaperones. Our results complemented previous immunoproteomics studies of SS2 strains.
authors.affiliation: College of Animal Science and Veterinary Medicine, Jilin University, Changchun, People's Republic of China.
authors.forename: Yingchao
authors.initials: Y
authors.lastname: Wang
authors.forename: Yuan
authors.initials: Y
authors.lastname: Dang
authors.forename: Xinglong
authors.initials: X
authors.lastname: Wang
authors.forename: Hao
authors.initials: H
authors.lastname: Lu
authors.forename: Xiuran
authors.initials: X
authors.lastname: Wang
authors.forename: Xulong
authors.initials: X
authors.lastname: Lang
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Li
authors.forename: Shuzhang
authors.initials: S
authors.lastname: Feng
authors.forename: Fuxian
authors.initials: F
authors.lastname: Zhang
authors.forename: Linzhu
authors.initials: L
authors.lastname: Ren
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis*; D002473:Cell Wall / Q000737:chemistry*; D015180:Electrophoresis, Gel, Two-Dimensional; D013058:Mass Spectrometry; D009154:Mutation; D020543:Proteome / Q000032:analysis*; D017011:Streptococcus suis / Q000737:chemistry* / Q000472:pathogenicity; D037521:Virulence Factors / Q000032:analysis
chemical_list: D001426:Bacterial Proteins; D020543:Proteome; D037521:Virulence Factors
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