Seson, a novel zinc finger protein, controls cilia integrity for the LR patterning during zebrafish embryogenesis.
In zebrafish embryos, bilateral symmetry is broken by asymmetric nodal flow generated in Kupffer's vesicle (KV), the transient cilia-rich organ, analogous to the mouse node. Asymmetric nodal flow induces the asymmetric expression of several genes, which are critical for the determination of correct LR body patterning. seson encoding three consecutive C2H2 zinc finger protein is predominantly expressed in the cilia-rich organs including KV. Inhibition of its function by the injection of a seson-specific MO inhibited the left-side biased expression of spaw, and resulted in randomization of the heart, gut looping and brain laterality. Disruption of the LR patterning in seson morphants appeared to be due to severe cilia defects in KV. Seson function was also required for ciliogenesis in other tissues such as the pronephros and olfactory organs. Collectively, our data suggest that Seson has critical roles in ciliogenesis and LR body axis patterning.
Kang
Nami
N
Department of Biology, Chungnam National University, Daejeon 305-764, Republic of Korea.
Ro
Hyunju
H
Park
Yonghoon
Y
Kim
Hyun-taek
HT
Huh
Tae-Lin
TL
Rhee
Myungchull
M
D000818:Animals; D019521:Body Patterning; D001921:Brain / Q000002:abnormalities / Q000196:embryology; D002352:Carrier Proteins / Q000235:genetics / Q000502:physiology*; D002923:Cilia / Q000502:physiology / Q000648:ultrastructure; D041981:Gastrointestinal Tract / Q000002:abnormalities / Q000196:embryology; D018507:Gene Expression Regulation, Developmental; D006321:Heart / Q000196:embryology; D013623:Tail / Q000002:abnormalities / Q000196:embryology; D015027:Zebrafish / Q000002:abnormalities / Q000196:embryology*; D029961:Zebrafish Proteins / Q000235:genetics / Q000502:physiology*; D016335:Zinc Fingers
D002352:Carrier Proteins; D029961:Zebrafish Proteins; C553749:jazf1b protein, zebrafish