Non-contact fluorescent bleaching-independent method for temperature measurement in microfluidic systems based on DNA melting curves.
This report introduces a bleaching-independent temperature measurement method based on the analysis of the fluorescence emitted during the melting of DNA molecules with the SYBR-Green I intercalator, in a microvolume where the strong non-linearity of the signal is used to eliminate the photobleaching effect as well as to determine the heat transfer rate between a heater and the sample and the temperature non-uniformity within the sample.
Neuzil
Pavel
P
Institute of Microelectronics, 11 Science Park Road, Singapore. pavel@kist-europe.de
Cheng
Fang
F
Soon
Jeffrey Bo Woon
JB
Qian
Lan Liu
LL
Reboud
Julien
J
D004247:DNA / Q000737:chemistry*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D008856:Microscopy, Fluorescence / Q000295:instrumentation*; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation*; D013821:Thermometers; D044366:Transition Temperature
D004247:DNA