title: Two antifungal components isolated from Fructus Psoraleae and Folium Eucalypti Globuli by bioassay-guided purification.
abstract: Fructus Psoraleae and Folium Eucalypti Globuli have long been used as Chinese medicines to treat various ailments such as asthma, eczema and dermatomycosis. In previous studies, their antifungal activities were demonstrated. The aim of the present study was to isolate active antidermatophytic compounds from their ethanolic extracts by means of bioassay-guided purification. Guided by the inhibitory activities on Trichophyton mentagrophytes, Trichophyton rubrum and Paecilomyces variotii, bakuchiol was isolated from the n-hexane fraction of Fructus Psoraleae whilst macrocarpal C was isolated from the n-hexane fraction of Folium Eucalypti Globuli. Both pure compounds could effectively inhibit the growth of dermatophytes in vitro. This is the first paper to report the isolation and identification of active antidermatophytic compounds from Fructus Psoraleae and Folium Eucalypti Globuli by the bioassay-guided purification.
authors.affiliation: Institute of Chinese Medicine, The Chinese University of Hong Kong, Hong Kong.
authors.forename: Kit-Man
authors.initials: KM
authors.lastname: Lau
authors.forename: Lai-Hong
authors.initials: LH
authors.lastname: Fu
authors.forename: Ling
authors.initials: L
authors.lastname: Cheng
authors.forename: Chun-Wai
authors.initials: CW
authors.lastname: Wong
authors.forename: Yin-Lai
authors.initials: YL
authors.lastname: Wong
authors.forename: Ching-Po
authors.initials: CP
authors.lastname: Lau
authors.forename: Simon Quan-Bin
authors.initials: SQ
authors.lastname: Han
authors.forename: Paul Kay-Sheung
authors.initials: PK
authors.lastname: Chan
authors.forename: Kwok-Pui
authors.initials: KP
authors.lastname: Fung
authors.forename: Clara Bik-San
authors.initials: CB
authors.lastname: Lau
authors.forename: Mamie
authors.initials: M
authors.lastname: Hui
authors.forename: Ping-Chung
authors.initials: PC
authors.lastname: Leung
mesh_terms: D000935:Antifungal Agents / Q000302:isolation & purification / Q000494:pharmacology*; D003883:Arthrodermataceae / Q000187:drug effects*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology*; D005052:Eucalyptus / Q000737:chemistry*; D005638:Fruit; D010143:Paecilomyces / Q000187:drug effects; D010636:Phenols / Q000302:isolation & purification / Q000494:pharmacology*; D010696:Phloroglucinol / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000494:pharmacology; D018515:Plant Leaves; D029907:Psoralea / Q000737:chemistry*; D012717:Sesquiterpenes / Q000302:isolation & purification / Q000494:pharmacology*; D014249:Trichophyton / Q000187:drug effects
chemical_list: D000935:Antifungal Agents; D004365:Drugs, Chinese Herbal; D010636:Phenols; D012717:Sesquiterpenes; C518773:macrocarpal C; D010696:Phloroglucinol; C012765:bakuchiol