title: Engineered lactic acid bacterium Lactococcus lactis capable of binding antibodies and tumor necrosis factor alpha.
abstract: We have optimized the display of the B domain of staphylococcal protein A on the surface of Lactococcus lactis. The maximum binding capacity was estimated at 0.146 μg of antibody per 10⁸ cells and was sustained at 86% after treatment with simulated gastric juice. A tumor necrosis factor alpha (TNF-α)-binding affibody was also displayed and bound TNF-α, which could be useful in the treatment of inflammatory bowel disease.
authors.affiliation: Department of Biotechnology, JoŽef Stefan Institute, Jamova 39, SI-1000 Ljubljana, Slovenia. ales.berlec@ijs.si.
authors.forename: Matjaz
authors.initials: M
authors.lastname: Ravnikar
authors.forename: Borut
authors.initials: B
authors.lastname: Strukelj
authors.forename: Natasa
authors.initials: N
authors.lastname: Obermajer
authors.forename: Mojca
authors.initials: M
authors.lastname: Lunder
authors.forename: Ales
authors.initials: A
authors.lastname: Berlec
mesh_terms: D000906:Antibodies / Q000378:metabolism*; D013294:Lactococcus lactis / Q000235:genetics* / Q000378:metabolism*; D011485:Protein Binding; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013205:Staphylococcal Protein A / Q000235:genetics* / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: D000906:Antibodies; D011994:Recombinant Proteins; D013205:Staphylococcal Protein A; D014409:Tumor Necrosis Factor-alpha
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