Effect of partial liquid ventilation on oleic acid-induced inflammatory responses in piglets.
BACKGROUND
Pediatric patients are susceptible to lung injury. Acute lung injury (ALI) in children often results in a high mortality. Partial liquid ventilation (PLV) has been shown to markedly improve oxygenation and reduce histologic evidence of injury in a number of lung injury models. This study aimed to examine the hypothesis that PLV would attenuate the production of local and systemic cytokines in an immature piglet model of ALI induced by oleic acid (OA).


METHODS
Twelve Chinese immature piglets were induced to develop ALI by oleic acid. The animals were randomly assigned to two groups (n = 6): (1) conventional mechanical ventilation (MV) group and (2) PLV with FC-77 (10 ml/kg) group.


RESULTS
Compared with MV group, PLV group got better cardiopulmonary variables (P < 0.05). These variables included heart rate, mean blood pressure, blood pH, partial pressure of arterial oxygen (PaO2), PaO2/FiO2 and partial pressure of arterial carbon dioxide (PaCO2). Partial liquid ventilation reduced IL-1beta, IL-6, IL-10 and TNF-alpha both in plasma and tissue concentrations compared with MV group (P < 0.05).


CONCLUSIONS
Partial liquid ventilation provides protective effects against inflammatory responses in the lungs of oleic acid-induced immature piglets.
Zhu
Yao-bin
YB
Department of Pediatric Cardiac Surgery Center, Fu Wai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100037, China.
Wang
Qiang
Q
Liu
Ying-long
YL
Li
Xiao-feng
XF
Li
Jian-an
JA
Lü
Xiao-dong
XD
Ling
Feng
F
Liu
Ai-jun
AJ
Fan
Xiang-ming
XM
D000818:Animals; D005466:Fluorocarbons / Q000627:therapeutic use; D006439:Hemodynamics / Q000187:drug effects; D007249:Inflammation / Q000139:chemically induced* / Q000628:therapy*; D016753:Interleukin-10 / Q000378:metabolism; D053583:Interleukin-1beta / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D021061:Liquid Ventilation / Q000379:methods*; D055370:Lung Injury / Q000276:immunology / Q000628:therapy; D019301:Oleic Acid / Q000633:toxicity*; D011897:Random Allocation; D012121:Respiration, Artificial; D013552:Swine; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D005466:Fluorocarbons; D053583:Interleukin-1beta; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; C429307:fluorocarbon 77; D016753:Interleukin-10; D019301:Oleic Acid