title: Multiplexed fluorescence lifetime measurements by frequency-sweeping Fourier spectroscopy.
abstract: We report simultaneous measurements of fluorescence lifetimes at multiple excitation wavelengths with a Fourier transform frequency domain fluorescence lifetime spectrometer. The spectrometer uses a Michelson interferometer with its differential optical path length scanning at a 22,000 Hz scan rate. The scan speed of the optical delay varies linearly during each scan and creates interference modulations that sweep from -150 to 150 MHz in 45.5 micros. The frequency-sweeping modulation allows nanosecond fluorescence lifetime measurements within 45.5 micros. Because the interference modulation frequency is wavelength dependent, under the Fourier multiplexing principle, the spectrometer can perform lifetime measurements on multiple excitation wavelengths simultaneously.
authors.affiliation: College of Optical Sciences, The University of Arizona, 1630 East University Boulevard, Tucson, Arizona 85721-0094, USA.
authors.forename: Ming
authors.initials: M
authors.lastname: Zhao
authors.forename: Leilei
authors.initials: L
authors.lastname: Peng
mesh_terms: D007368:Interferometry; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*; D013997:Time Factors
references.citation: Appl Opt. 2003 Aug 1;42(22):4606-11
references.pmid: 12916629
references.citation: J Opt Soc Am A Opt Image Sci Vis. 2008 Feb;25(2):452-62
references.pmid: 18246179
references.citation: Photochem Photobiol Sci. 2005 Jan;4(1):13-22
references.pmid: 15616687
references.citation: Biophys J. 2005 Apr;88(4):2939-53
references.pmid: 15653725
references.citation: Curr Opin Chem Biol. 2003 Oct;7(5):635-40
references.pmid: 14580569
references.citation: Anal Chem. 2007 Mar 1;79(5):2137-49
references.pmid: 17269654
references.citation: Opt Lett. 2007 Feb 15;32(4):421-3
references.pmid: 17356673
references.citation: Opt Express. 2008 Jul 7;16(14):10493-500
references.pmid: 18607462
references.citation: Histochem Cell Biol. 2008 Nov;130(5):833-43
references.pmid: 18830616
references.citation: Opt Lett. 2007 Dec 1;32(23):3408-10
references.pmid: 18059949