title: Specific cell ablation in Drosophila using the toxic viral protein M2(H37A).
abstract: The expression of toxic viral proteins for the purpose of eliminating distinct populations of cells, while leaving the rest of an organism unaffected, is a valuable method for analyzing development. Using the Gal4-UAS system, we employed the M2(H37A) toxic ion channel of the influenza-A virus to selectively ablate the Drosophila eye-antennal imaginal discs, hemocytes, dorsal vessel and nervous tissue, and comparatively monitored the effects of expressing the apoptosis-promoting protein Reaper in identical cell populations. In this report, we demonstrate the effectiveness of M2(H37A)-mediated ablation as a new means to selectively eliminate cells of interest during Drosophila development.
authors.affiliation: Department of Biological Sciences, University of Notre Dame, Notre Dame, Indiana, USA.
authors.forename: Victoria K
authors.initials: VK
authors.lastname: Lam
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Tokusumi
authors.forename: Donna
authors.initials: D
authors.lastname: Cerabona
authors.forename: Robert A
authors.initials: RA
authors.lastname: Schulz
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified / Q000254:growth & development / Q000378:metabolism; D017209:Apoptosis; D004330:Drosophila / Q000166:cytology / Q000235:genetics* / Q000254:growth & development; D005123:Eye / Q000166:cytology; D005818:Genetic Engineering / Q000379:methods*; D006434:Hemocytes / Q000166:cytology / Q000378:metabolism; D007473:Ion Channels / Q000235:genetics*; D007814:Larva / Q000166:cytology / Q000235:genetics / Q000254:growth & development; D058953:Neural Stem Cells / Q000166:cytology / Q000378:metabolism; D009975:Orthomyxoviridae / Q000235:genetics; D010641:Phenotype; D014763:Viral Matrix Proteins / Q000235:genetics*; D000086082:Viroporin Proteins
chemical_list: D007473:Ion Channels; D014763:Viral Matrix Proteins; C488937:M2 protein, Influenza A virus; D000086082:Viroporin Proteins
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