Effect of granulocyte-colony stimulating factor on spinal cord tissue after experimental contusion injury.
The purpose of this study was to investigate the early effects of granulocyte-colony stimulating factor (G-CSF) on myeloperoxidase (MPO) activity, lipid peroxidation (LPO) and ultrastructural findings in rats after spinal cord injury (SCI). We also compared the effects of G-CSF and methylprednisolone sodium succinate (MPSS). Wistar rats were divided into four groups: control, SCI alone (50 g/cm weight drop trauma), SCI+MPSS (30 mg/kg), and SCI+G-CSF (50 μg/kg). Administration of G-CSF and MPSS significantly decreased LPO (p < 0.05) and MPO activity (p < 0.05) in the first 24 hours. MPSS was more effective than G-CSF in reducing LPO (p < 0.05) and in minimizing ultrastructure changes. The results of this study indicate that G-CSF exerts a beneficial effect by decreasing MPO activity and LPO and may reduce tissue damage in the first 24 hours after SCI. Our findings do not exclude the possibility that G-CSF has a protective effect on spinal cord ultrastructure after the first 24 hours following SCI.
Sanli
A Metin
AM
Department of Neurosurgery, Dişkapi Yildirım Beyazit Research and Education Hospital, Ankara, Turkey. ahmetmetinsanli@hotmail.com
Serbes
Gökhan
G
Calişkan
Murat
M
Kaptanoğlu
Erkan
E
Sargon
Mustafa F
MF
Kilinç
Kamer
K
Beşalti
Omer
O
Sekerci
Zeki
Z
D000818:Animals; D001369:Axons / Q000187:drug effects / Q000648:ultrastructure; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects*; D008776:Methylprednisolone Hemisuccinate / Q000494:pharmacology; D046529:Microscopy, Electron, Transmission; D008928:Mitochondria / Q000187:drug effects / Q000648:ultrastructure; D018696:Neuroprotective Agents / Q000494:pharmacology*; D009195:Peroxidase / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013119:Spinal Cord Injuries / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology*
D018696:Neuroprotective Agents; D008776:Methylprednisolone Hemisuccinate; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D009195:Peroxidase