The Corynebacterium glutamicum aconitase repressor: scratching around for crystals.
Imperfections on the surfaces of crystallization containers are known to influence crystal formation and are thought to do so by helping to overcome the nucleation barrier. The intentional creation of imperfections has been widely applied to induce crystallization of small molecules, but has not been reported for protein crystallization. Here, the crystallization and preliminary X-ray analysis of the TetR-type aconitase repressor are reported. This regulator was the first transcription factor to be identified in the regulation of the tricarboxylic acid cycle in Corynebacterium glutamicum, an organism that is of special industrial interest and is an emerging model organism for Corynebacterineae. Successful crystallization involved introducing manual scratches on the surface of standard commercial plates, which led to a substantial improvement in crystal nucleation and quality.
García-Nafría
Javier
J
Structural Biology Laboratory, Department of Chemistry, University of York, York YO10 5DD, England.
Baumgart
Meike
M
Bott
Michael
M
Wilkinson
Anthony J
AJ
Wilson
Keith S
KS
D000154:Aconitate Hydratase / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D048230:Corynebacterium glutamicum / Q000737:chemistry* / Q000378:metabolism; D003460:Crystallization
D001426:Bacterial Proteins; D000154:Aconitate Hydratase
J Biol Chem. 2005 Jan 7;280(1):585-95
15494411
Nat Methods. 2008 Feb;5(2):147-53
18235435
Trends Biotechnol. 2009 Feb;27(2):99-106
19110330
Microbiol Mol Biol Rev. 2005 Jun;69(2):326-56
15944459
Acta Crystallogr D Biol Crystallogr. 2002 Oct;58(Pt 10 Pt 1):1649-52
12351879
Science. 1988 Jan 22;239(4838):385-7
17836869
Trends Microbiol. 2007 Sep;15(9):417-25
17764950
J Biotechnol. 2003 Sep 4;104(1-3):155-72
12948636
Methods Enzymol. 1997;276:307-26
27754618
J Struct Biol. 2003 Apr;142(1):218-31
12718933
J Cell Biochem. 2002;85(2):243-51
11948680
Acta Crystallogr D Biol Crystallogr. 1999 Oct;55(Pt 10):1696-702
10531519
PLoS One. 2009;4(1):e4198
19145241
J Phys Chem B. 2007 Dec 20;111(50):13971-8
18044862