title: High-speed crystal detection and characterization using a fast-readout detector.
abstract: A novel raster-scanning method combining continuous sample translation with the fast readout of a Pilatus P6M detector has been developed on microfocus beamline I24 at Diamond Light Source. This fast grid-scan tool allows the rapid evaluation of large sample volumes without the need to increase the beam size at the sample through changes in beamline hardware. A slow version is available for slow-readout detectors. Examples of grid-scan use in centring optically invisible samples and in detecting and characterizing numerous microcrystals on a mesh-like holder illustrate the most common applications of the grid scan now in routine use on I24.
authors.affiliation: Diamond Light Source, Harwell Science and Innovation Campus, Didcot, England.
authors.forename: Jun
authors.initials: J
authors.lastname: Aishima
authors.forename: Robin L
authors.initials: RL
authors.lastname: Owen
authors.forename: Danny
authors.initials: D
authors.lastname: Axford
authors.forename: Emma
authors.initials: E
authors.lastname: Shepherd
authors.forename: Graeme
authors.initials: G
authors.lastname: Winter
authors.forename: Karl
authors.initials: K
authors.lastname: Levik
authors.forename: Paul
authors.initials: P
authors.lastname: Gibbons
authors.forename: Alun
authors.initials: A
authors.lastname: Ashton
authors.forename: Gwyndaf
authors.initials: G
authors.lastname: Evans
mesh_terms: D018360:Crystallography, X-Ray / Q000295:instrumentation / Q000379:methods*; D013997:Time Factors
references.citation: Curr Opin Struct Biol. 2005 Oct;15(5):556-62
references.pmid: 16168633
references.citation: Acta Crystallogr D Biol Crystallogr. 2006 Oct;62(Pt 10):1162-9
references.pmid: 17001093
references.citation: Curr Opin Struct Biol. 2000 Aug;10(4):486-97
references.pmid: 10981640
references.citation: J Synchrotron Radiat. 2007 Mar;14(Pt 2):191-5
references.pmid: 17317920
references.citation: J R Soc Interface. 2009 Oct 6;6 Suppl 5:S587-97
references.pmid: 19535414
references.citation: EMBO J. 2010 Jan 20;29(2):505-14
references.pmid: 19959989