title: Elucidation of triacylglycerols in cod liver oil by liquid chromatography electrospray tandem ion-trap mass spectrometry.
abstract: Though liquid chromatography electrospray tandem mass spectrometry (LC-ESI-MS(2)) has been widely used in the structural elucidation of triacylglycerols (TAG) in vegetable oils, its potentiality for the identification of TAG molecules in omega-3 rich oils remains unexplored till date. Hence, this article investigates the applicability of LC-ESI-MS(2) for the structural characterization of naturally occurring TAG in cod liver oil without the TAG fractionation during the sample preparation. A computational algorithm was developed to automatically interpret the mass spectra and elucidate the TAG structures respectively. The results were compared against the lipase benchmark method. A principal component analysis study revealed that it is possible to discriminate genuine from adulterated cod liver oil.
authors.affiliation: National Institute of Nutrition and Seafood Research NIFES, PO Box 2029, Nordnes, N-5817 Bergen, Norway.
authors.forename: Ying-Xu
authors.initials: YX
authors.lastname: Zeng
authors.forename: Pedro
authors.initials: P
authors.lastname: Araujo
authors.forename: Zhen-Yu
authors.initials: ZY
authors.lastname: Du
authors.forename: Thu-Thao
authors.initials: TT
authors.lastname: Nguyen
authors.forename: Livar
authors.initials: L
authors.lastname: Frøyland
authors.forename: Bjørn
authors.initials: B
authors.lastname: Grung
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D003060:Cod Liver Oil / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D014280:Triglycerides / Q000032:analysis*
chemical_list: D014280:Triglycerides; D003060:Cod Liver Oil