Phosphoenolpyruvate provision to plastids is essential for gametophyte and sporophyte development in Arabidopsis thaliana.
Restriction of phosphoenolpyruvate (PEP) supply to plastids causes lethality of female and male gametophytes in Arabidopsis thaliana defective in both a phosphoenolpyruvate/phosphate translocator (PPT) of the inner envelope membrane and the plastid-localized enolase (ENO1) involved in glycolytic PEP provision. Homozygous double mutants of cue1 (defective in PPT1) and eno1 could not be obtained, and homozygous cue1 heterozygous eno1 mutants [cue1/eno1(+/-)] exhibited retarded vegetative growth, disturbed flower development, and up to 80% seed abortion. The phenotypes of diminished oil in seeds, reduced flavonoids and aromatic amino acids in flowers, compromised lignin biosynthesis in stems, and aberrant exine formation in pollen indicate that cue1/eno1(+/-) disrupts multiple pathways. While diminished fatty acid biosynthesis from PEP via plastidial pyruvate kinase appears to affect seed abortion, a restriction in the shikimate pathway affects formation of sporopollonin in the tapetum and lignin in the stem. Vegetative parts of cue1/eno1(+/-) contained increased free amino acids and jasmonic acid but had normal wax biosynthesis. ENO1 overexpression in cue1 rescued the leaf and root phenotypes, restored photosynthetic capacity, and improved seed yield and oil contents. In chloroplasts, ENO1 might be the only enzyme missing for a complete plastidic glycolysis.
Prabhakar
Veena
V
Universität zu Köln, Biozentrum, Botanisches Institut II, D-50674 Cologne, Germany.
Löttgert
Tanja
T
Geimer
Stefan
S
Dörmann
Peter
P
Krüger
Stephan
S
Vijayakumar
Vinod
V
Schreiber
Lukas
L
Göbel
Cornelia
C
Feussner
Kirstin
K
Feussner
Ivo
I
Marin
Kay
K
Staehr
Pia
P
Bell
Kirsten
K
Flügge
Ulf-Ingo
UI
Häusler
Rainer E
RE
D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D003433:Crosses, Genetic; D018506:Gene Expression Regulation, Plant; D055786:Gene Knockout Techniques; D055993:Germ Cells, Plant / Q000254:growth & development*; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D009154:Mutation; D010641:Phenotype; D010728:Phosphoenolpyruvate / Q000378:metabolism*; D010751:Phosphopyruvate Hydratase / Q000235:genetics / Q000378:metabolism; D018087:Plastids / Q000235:genetics / Q000378:metabolism*; D011058:Pollen / Q000648:ultrastructure
D029681:Arabidopsis Proteins; C491608:CUE1 protein, Arabidopsis; D026901:Membrane Transport Proteins; C481826:phosphate translocator 1, Arabidopsis; D010728:Phosphoenolpyruvate; D010751:Phosphopyruvate Hydratase
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