CCL20 is overexpressed in Mycobacterium tuberculosis-infected monocytes and inhibits the production of reactive oxygen species (ROS).
CCL20 is a chemokine that attracts immature dendritic cells. We show that monocytes, cells characteristic of the innate immune response, infected with Mycobacterium tuberculosis express the CCL20 gene at a much higher level than the same cells infected with non-tuberculous mycobacteria. Interferon (IFN)-γ, a fundamental cytokine in the immune response to tuberculosis, strongly inhibits both the transcription and the translation of CCL20. We have also confirmed that dendritic cells are a suitable host for mycobacteria proliferation, although CCL20 does not seem to influence their intracellular multiplication rate. The chemokine, however, down-regulates the characteristic production of reactive oxygen species (ROS) induced by M. tuberculosis in monocytes, which may affect the activity of the cells. Apoptosis mediated by the mycobacteria, possibly ROS-dependent, was also inhibited by CCL20.
Rivero-Lezcano
O M
OM
Fundación Instituto de Estudios de Ciencias de la Salud de Castilla y León, Hospital de León, Spain. omrivero@iecscyl.com
González-Cortés
C
C
Reyes-Ruvalcaba
D
D
Diez-Tascón
C
C
D000906:Antibodies / Q000276:immunology / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects / Q000276:immunology; D018932:Chemokine CCL2 / Q000276:immunology / Q000378:metabolism; D054418:Chemokine CCL20 / Q000235:genetics / Q000276:immunology / Q000378:metabolism* / Q000494:pharmacology; D019742:Chemokines, CC / Q000235:genetics; D002633:Chemotaxis / Q000187:drug effects / Q000276:immunology; D015169:Colony Count, Microbial; D017077:Culture Media, Conditioned / Q000494:pharmacology; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology / Q000382:microbiology; D015870:Gene Expression / Q000235:genetics; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D016952:Legionella pneumophila / Q000166:cytology / Q000276:immunology; D008264:Macrophages / Q000187:drug effects / Q000276:immunology / Q000382:microbiology; D009000:Monocytes / Q000187:drug effects / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology*; D009162:Mycobacterium avium / Q000166:cytology / Q000276:immunology; D019909:Mycobacterium kansasii / Q000166:cytology / Q000276:immunology; D009169:Mycobacterium tuberculosis / Q000166:cytology / Q000276:immunology*; D017382:Reactive Oxygen Species / Q000378:metabolism*
D000906:Antibodies; C111835:CCL2 protein, human; C108355:CCL20 protein, human; D018932:Chemokine CCL2; D054418:Chemokine CCL20; D019742:Chemokines, CC; D017077:Culture Media, Conditioned; D017382:Reactive Oxygen Species; D007371:Interferon-gamma
World J Gastroenterol. 2008 Sep 28;14(36):5570-83; discussion 5581-2
18810777
Infect Immun. 2007 Nov;75(11):5223-32
17724069
Curr Opin Pulm Med. 2001 May;7(3):133-41
11371768
PLoS Pathog. 2008 Dec;4(12):e1000229
19057661
J Exp Med. 2008 May 12;205(5):1121-32
18426984
Cell Microbiol. 2009 Aug;11(8):1170-8
19438516
Cytokine Growth Factor Rev. 2003 Dec;14(6):467-77
14563349
Clin Exp Immunol. 2005 Nov;142(2):285-91
16232215
J Leukoc Biol. 1994 Jun;55(6):756-62
7515094
Yonsei Med J. 2009 Feb 28;50(1):1-11
19259342
Tuberculosis (Edinb). 2005 Jan-Mar;85(1-2):53-64
15687028
Annu Rev Immunol. 2000;18:217-42
10837058
Microbes Infect. 2006 Apr;8(5):1339-46
16697232
Cytokine Growth Factor Rev. 2003 Oct;14(5):409-26
12948524
Clin Immunol. 2002 Dec;105(3):296-303
12498811
J Immunol. 2003 Feb 15;170(4):1939-48
12574362
J Exp Med. 2005 Dec 19;202(12):1649-58
16352737
Infect Immun. 2008 Apr;76(4):1719-27
18212080
J Invest Dermatol. 2005 Oct;125(4):615-28
16185259
J Immunol. 2005 Apr 1;174(7):4203-9
15778382
Infect Immun. 2000 Oct;68(10):5824-9
10992491
Scand J Immunol. 2008 Jan;67(1):77-85
18052967
J Immunol. 2005 Jul 15;175(2):874-83
16002685
J Clin Immunol. 2007 Jul;27(4):347-62
17364232
J Immunol. 2000 Jul 15;165(2):978-87
10878374
J Immunol. 2004 Aug 1;173(3):2060-7
15265941
Biochem Biophys Res Commun. 1998 Jun 18;247(2):436-42
9642146
Nucleic Acids Res. 2001 May 1;29(9):e45
11328886
Chin Med J (Engl). 2000 Dec;113(12):1124-8
11776150
J Immunol. 2002 Jun 15;168(12):6358-65
12055253
IUBMB Life. 2001 Jun;51(6):365-71
11758804
J Immunol. 2007 Aug 15;179(4):2509-19
17675513
FEMS Microbiol Immunol. 1992 Dec;5(5-6):355-62
1466909
Apoptosis. 2000 Nov;5(5):415-8
11256882
Infect Immun. 2005 Jun;73(6):3330-41
15908359
J Exp Med. 2001 Oct 15;194(8):1123-40
11602641
J Biosci. 2008 Mar;33(1):137-44
18376078