title: Two Japanese Corynebacterium ulcerans isolates from the same hospital: ribotype, toxigenicity and serum antitoxin titre.
abstract: Two toxigenic Corynebacterium ulcerans isolates recovered from pharyngeal swabs of two patients from the same hospital in Japan during 2001-2002 were characterized by PFGE and ribotyping. Toxin production in different culture media was examined and serological analysis of patient sera was performed. The two isolates could not be distinguished by PFGE; however, their ribotypes were distinguishable. One of the isolates could represent a novel ribotype. Analysis of toxin production in different culture media demonstrated that the two isolates produced varying amounts of the diphtheria toxin. Serological analysis showed a greater than sevenfold increase in the serum antitoxin titre during the course of infection in one patient.
authors.affiliation: Department of Bacteriology II, National Institute of Infectious Diseases, Tokyo, Japan.
authors.forename: Takako
authors.initials: T
authors.lastname: Komiya
authors.affiliation: Infectious Diseases Control, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Osaka, Japan.
authors.forename: Yukiji
authors.initials: Y
authors.lastname: Seto
authors.affiliation: Streptococcus and Diphtheria Reference Unit, Respiratory and Systemic Infections Department, Health Protection Agency Centre for Infections, London, UK.
authors.forename: Aruni
authors.initials: A
authors.lastname: De Zoysa
authors.affiliation: Department of Bacteriology II, National Institute of Infectious Diseases, Tokyo, Japan.
authors.forename: Masaaki
authors.initials: M
authors.lastname: Iwaki
authors.affiliation: Asahi General Hospital, Chiba, Japan.
authors.forename: Akio
authors.initials: A
authors.lastname: Hatanaka
authors.affiliation: Asahi General Hospital, Chiba, Japan.
authors.forename: Atsunobu
authors.initials: A
authors.lastname: Tsunoda
authors.affiliation: Department of Bacteriology II, National Institute of Infectious Diseases, Tokyo, Japan.
authors.forename: Yoshichika
authors.initials: Y
authors.lastname: Arakawa
authors.affiliation: Infectious Diseases Control, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Osaka, Japan.
authors.forename: Shunji
authors.initials: S
authors.lastname: Kozaki
authors.affiliation: Department of Bacteriology II, National Institute of Infectious Diseases, Tokyo, Japan.
authors.forename: Motohide
authors.initials: M
authors.lastname: Takahashi
mesh_terms: D000992:Antitoxins / Q000097:blood*; D001427:Bacterial Toxins; D003352:Corynebacterium / Q000145:classification* / Q000302:isolation & purification*; D003354:Corynebacterium Infections / Q000453:epidemiology / Q000382:microbiology*; D005260:Female; D005838:Genotype; D006801:Humans; D007564:Japan / Q000453:epidemiology; D008297:Male; D008875:Middle Aged; D021521:Ribotyping
chemical_list: D000992:Antitoxins; D001427:Bacterial Toxins