Modulation of potassium channels via the α1B-adrenergic receptor in human osteoblasts.
Recent studies have demonstrated the involvement of the sympathetic nerve system in bone metabolism. We have previously demonstrated the expression of adrenergic receptors in the human osteoblast SaM-1 cell line. The aim of this study was to reveal the function of these receptors in osteoblasts using electrophysiological methods. During whole-cell patch clamp recording, the application of noradrenaline reduced the currents induced by a voltage ramp, and the effect was larger in the positive potential range. Pretreatment with the selective α(1B)-adrenergic receptor antagonist chloroethylclonidine eliminated the inhibitory effect of noradrenaline. Using Cs-based pipette solution to block potassium channels, the inhibitory effect of noradrenaline disappeared. In contrast, the effect was unaffected in the presence of tetraethylammonium (TEA), a potassium channel blocker. These results suggest that noradrenaline suppresses Cs-sensitive and TEA-insensitive potassium channels via the α(1B)-adrenergic receptor in human osteoblasts.
Kodama
Daisuke
D
Department of Pharmacology, School of Dentistry, Aichi-Gakuin University, 1-100 Kusumoto-cho, Chikusa-ku, Nagoya 464-8650, Japan.
Togari
Akifumi
A
D000317:Adrenergic alpha-Antagonists / Q000494:pharmacology; D002460:Cell Line; D003000:Clonidine / Q000031:analogs & derivatives / Q000494:pharmacology; D006801:Humans; D008297:Male; D008564:Membrane Potentials / Q000502:physiology*; D009638:Norepinephrine / Q000378:metabolism / Q000502:physiology; D010006:Osteoblasts / Q000187:drug effects / Q000378:metabolism*; D018408:Patch-Clamp Techniques / Q000379:methods; D026902:Potassium Channel Blockers / Q000494:pharmacology; D015221:Potassium Channels / Q000378:metabolism*; D018340:Receptors, Adrenergic, alpha-1 / Q000502:physiology*; D055815:Young Adult
D000317:Adrenergic alpha-Antagonists; D026902:Potassium Channel Blockers; D015221:Potassium Channels; D018340:Receptors, Adrenergic, alpha-1; C051731:chlorethylclonidine; D003000:Clonidine; D009638:Norepinephrine