title: Renal ischemia regulates marinobufagenin release in humans.
abstract: Cardiotonic steroids, including marinobufagenin, are a group of new steroid hormones found in plasma and urine of patients with congestive heart failure, myocardial infarction, and chronic renal failure. In animal studies, partial nephrectomy induces marinobufagenin elevation, cardiac hypertrophy, and fibrosis. The objective of this study is to test the effect of renal ischemia on marinobufagenin levels in humans with renal artery stenosis (RAS). To test this, plasma marinobufagenin levels were measured in patients with RAS of the Prospective Randomized Study Comparing Renal Artery Stenting With or Without Distal Protection, non-RAS patient controls who were scheduled for coronary angiography, and normal healthy individuals. Marinobufagenin levels were significantly higher in patients with RAS compared with those of the other 2 groups. Multivariate analysis shows that occurrence of RAS is independently related to marinobufagenin levels. In addition, renal artery revascularization by stenting partially reversed marinobufagenin levels in the patients with RAS (0.77±0.06 nmol/L at baseline; 0.66±0.06 nmol/L at 24 hours; and 0.61±0.05 nmol/L at 1 month). In conclusion, we have found that marinobufagenin levels are increased in patients with RAS, whereas reversal of renal ischemia by stenting treatment reduces marinobufagenin levels. These results suggest that RAS-induced renal ischemia may be a major cause of marinobufagenin release.
authors.affiliation: Department of Medicine Cardiovascular Division, University of Toledo, Toledo, OH 43614, USA. Jiang.tian@utoledo.edu
authors.forename: Jiang
authors.initials: J
authors.lastname: Tian
authors.forename: Steven
authors.initials: S
authors.lastname: Haller
authors.forename: Sankaridrug
authors.initials: S
authors.lastname: Periyasamy
authors.forename: Pamela
authors.initials: P
authors.lastname: Brewster
authors.forename: Haifeng
authors.initials: H
authors.lastname: Zhang
authors.forename: Satjit
authors.initials: S
authors.lastname: Adlakha
authors.forename: Olga V
authors.initials: OV
authors.lastname: Fedorova
authors.forename: Zi-Jian
authors.initials: ZJ
authors.lastname: Xie
authors.forename: Alexei Y
authors.initials: AY
authors.lastname: Bagrov
authors.forename: Joseph I
authors.initials: JI
authors.lastname: Shapiro
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Cooper
mesh_terms: D000368:Aged; D000704:Analysis of Variance; D002018:Bufanolides / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005919:Glomerular Filtration Rate; D006801:Humans; D006973:Hypertension / Q000097:blood* / Q000150:complications; D007511:Ischemia / Q000097:blood*; D007668:Kidney / Q000098:blood supply*; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D018579:Patient Selection; D011446:Prospective Studies; D012078:Renal Artery Obstruction / Q000097:blood* / Q000628:therapy; D015607:Stents
chemical_list: D002018:Bufanolides; C093896:marinobufagenin
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