title: A conserved acetyl esterase domain targets diverse bacteriophages to the Vi capsular receptor of Salmonella enterica serovar Typhi.
abstract: A number of bacteriophages have been identified that target the Vi capsular antigen of Salmonella enterica serovar Typhi. Here we show that these Vi phages represent a remarkably diverse set of phages belonging to three phage families, including Podoviridae and Myoviridae. Genome analysis facilitated the further classification of these phages and highlighted aspects of their independent evolution. Significantly, a conserved protein domain carrying an acetyl esterase was found to be associated with at least one tail fiber gene for all Vi phages, and the presence of this domain was confirmed in representative phage particles by mass spectrometric analysis. Thus, we provide a simple explanation and paradigm of how a diverse group of phages target a single key virulence antigen associated with this important human-restricted pathogen.
authors.affiliation: Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Sulston Building, Hinxton, Cambridge CB10 1SA, United Kingdom. djp@sanger.ac.uk
authors.forename: Derek
authors.initials: D
authors.lastname: Pickard
authors.forename: Ana Luisa
authors.initials: AL
authors.lastname: Toribio
authors.forename: Nicola K
authors.initials: NK
authors.lastname: Petty
authors.forename: Andries
authors.initials: A
authors.lastname: van Tonder
authors.forename: Lu
authors.initials: L
authors.lastname: Yu
authors.forename: David
authors.initials: D
authors.lastname: Goulding
authors.forename: Bart
authors.initials: B
authors.lastname: Barrell
authors.forename: Richard
authors.initials: R
authors.lastname: Rance
authors.forename: David
authors.initials: D
authors.lastname: Harris
authors.forename: Michael
authors.initials: M
authors.lastname: Wetter
authors.forename: John
authors.initials: J
authors.lastname: Wain
authors.forename: Jyoti
authors.initials: J
authors.lastname: Choudhary
authors.forename: Nicholas
authors.initials: N
authors.lastname: Thomson
authors.forename: Gordon
authors.initials: G
authors.lastname: Dougan
mesh_terms: D000115:Acetylesterase / Q000235:genetics / Q000378:metabolism*; D015967:Gene Expression Regulation, Viral / Q000502:physiology*; D016679:Genome, Viral; D008969:Molecular Sequence Data; D011135:Polysaccharides, Bacterial / Q000502:physiology*; D017434:Protein Structure, Tertiary; D012483:Salmonella Phages / Q000235:genetics / Q000378:metabolism*; D012485:Salmonella typhi / Q000378:metabolism*; D026801:Synteny; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D011135:Polysaccharides, Bacterial; D014764:Viral Proteins; C054154:capsular polysaccharide, Salmonella; D000115:Acetylesterase
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