Specific cell ablation in Drosophila using the toxic viral protein M2(H37A).
The expression of toxic viral proteins for the purpose of eliminating distinct populations of cells, while leaving the rest of an organism unaffected, is a valuable method for analyzing development. Using the Gal4-UAS system, we employed the M2(H37A) toxic ion channel of the influenza-A virus to selectively ablate the Drosophila eye-antennal imaginal discs, hemocytes, dorsal vessel and nervous tissue, and comparatively monitored the effects of expressing the apoptosis-promoting protein Reaper in identical cell populations. In this report, we demonstrate the effectiveness of M2(H37A)-mediated ablation as a new means to selectively eliminate cells of interest during Drosophila development.
Lam
Victoria K
VK
Department of Biological Sciences, University of Notre Dame, Notre Dame, Indiana, USA.
Tokusumi
Tsuyoshi
T
Cerabona
Donna
D
Schulz
Robert A
RA
D000818:Animals; D030801:Animals, Genetically Modified / Q000254:growth & development / Q000378:metabolism; D017209:Apoptosis; D004330:Drosophila / Q000166:cytology / Q000235:genetics* / Q000254:growth & development; D005123:Eye / Q000166:cytology; D005818:Genetic Engineering / Q000379:methods*; D006434:Hemocytes / Q000166:cytology / Q000378:metabolism; D007473:Ion Channels / Q000235:genetics*; D007814:Larva / Q000166:cytology / Q000235:genetics / Q000254:growth & development; D058953:Neural Stem Cells / Q000166:cytology / Q000378:metabolism; D009975:Orthomyxoviridae / Q000235:genetics; D010641:Phenotype; D014763:Viral Matrix Proteins / Q000235:genetics*; D000086082:Viroporin Proteins
D007473:Ion Channels; D014763:Viral Matrix Proteins; C488937:M2 protein, Influenza A virus; D000086082:Viroporin Proteins
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