Growth suppression of human laryngeal squamous cell carcinoma by adenoviral-mediated interleukin-12.
This study explored the inhibitory role of the adenoviral-mediated-interleukin (IL)-12 (Ad.mIL-12) gene in the growth of laryngeal squamous cell carcinoma (LSCC). Human epithelial type 2 (Hep-2) cells were transfected with Ad.mIL-12, and IL-12 gene expression of the cells was evaluated. The proliferation and apoptosis of Hep-2 cells in vitro were detected by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and flow cytometry. Experimental tumours in mice were injected intratumourally with the same recombinant adenoviruses and inhibition of tumour growth observed. Apoptosis in Hep-2 xenotransplants was detected using TUNEL (terminal deoxynucleotidyl transferase dUTP nick-end labelling) assay and transmission electron microscopy. The expression of IL-12 in Ad.mIL-12 transfected Hep-2 cells was significantly increased. In vitro, Ad.mIL-12 decreased the viability of and increased apoptosis in Hep-2 cells. Increased apoptosis was also seen in vivo. The mean weight and volume of tumours in Ad.mIL-12 treated mice were significantly lower than in the control group. It is concluded that Ad.mIL-12 can suppress LSCC growth and induce apoptosis.
Tian
L
L
Department of Otorhinolaryngology/Head and Neck Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.
Chen
X
X
Sun
Y
Y
Liu
M
M
Zhu
D
D
Ren
J
J
D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D017209:Apoptosis; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000473:pathology* / Q000628:therapy; D002470:Cell Survival; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors; D056945:Hep G2 Cells / Q000648:ultrastructure / Q000821:virology; D006801:Humans; D018664:Interleukin-12 / Q000235:genetics / Q000378:metabolism; D007822:Laryngeal Neoplasms / Q000378:metabolism / Q000473:pathology* / Q000628:therapy; D051379:Mice; D008807:Mice, Inbred BALB C; D046529:Microscopy, Electron, Transmission; D013778:Tetrazolium Salts / Q000378:metabolism; D013844:Thiazoles / Q000378:metabolism; D014162:Transfection; D023041:Xenograft Model Antitumor Assays
D013778:Tetrazolium Salts; D013844:Thiazoles; D018664:Interleukin-12; C022616:thiazolyl blue