The phrenic motor nucleus in the adult mouse.
The present study was performed to establish an anatomical context for studies of phrenic motor function in mouse models of central nervous system trauma and disease. Application of cholera toxin β-subunit to the diaphragm of adult C57BL/6 mice revealed a columnar organization of phrenic motoneurons (PhMNs) which extended from rostral C3 to C6. Injection of Miniruby into the ventrolateral medulla revealed decussating, anterogradely labeled axons in the cervical spinal cord. In addition, application of the transneuronal tracer pseudorabies virus (PRV) to the right hemidiaphragm demonstrated a population of putative pre-phrenic interneurons at the level of the infected PhMN pool. These neuroanatomical features of the mouse phrenic nucleus are consistent with those described in other species and provide a foundation for studies of neuroplasticity and repair in relation to a functionally and anatomically identified spinal network.
Qiu
K
K
University of Florida, College of Public Health and Health Professions, McKnight Brain Institute, Department of Physical Therapy, PO Box 100154, 100 Newell Dr, Gainesville, FL 32610, USA.
Lane
M A
MA
Lee
K Z
KZ
Reier
P J
PJ
Fuller
D D
DD
D000818:Animals; D001710:Biotin / Q000031:analogs & derivatives; D002772:Cholera Toxin / Q000633:toxicity; D003911:Dextrans; D005456:Fluorescent Dyes; D007150:Immunohistochemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D009046:Motor Neurons / Q000502:physiology*; D009415:Nerve Net / Q000166:cytology / Q000502:physiology; D009473:Neuronal Plasticity / Q000502:physiology; D010791:Phrenic Nerve / Q000166:cytology / Q000502:physiology*; D012235:Rhodamines; D013116:Spinal Cord / Q000473:pathology
D003911:Dextrans; D005456:Fluorescent Dyes; D012235:Rhodamines; C081276:Miniruby; D001710:Biotin; D002772:Cholera Toxin
Respir Physiol Neurobiol. 2009 Nov 30;169(2):141-9
19651246
J Comp Neurol. 1991 Jun 8;308(2):169-79
1716267
Mol Cell Neurosci. 2008 Jan;37(1):143-52
18042398
Anat Anz. 1980;148(1):49-54
7212282
Exp Neurol. 2006 Aug;200(2):486-95
16631169
J Comp Neurol. 2008 Dec 10;511(5):692-709
18924146
Respir Physiol Neurobiol. 2009 Nov 30;169(2):94-101
19560562
Neurosci Lett. 2007 May 1;417(2):206-11
17412505
Exp Brain Res. 1979 May 2;35(3):395-406
456448
J Anat. 1985 May;140 ( Pt 3):491-8
4066485
J Comp Neurol. 1956 Oct;105(3):553-85
13385384
J Appl Physiol (1985). 2003 Feb;94(2):795-810
12531916
J Neurocytol. 1984 Feb;13(1):85-109
6707715
J Comp Neurol. 1981 Sep 20;201(3):441-56
7276259
J Neurosci Methods. 2009 Sep 15;182(2):244-9
19559048
Exp Neurol. 1984 Dec;86(3):559-75
6209160
Exp Neurol. 2005 Aug;194(2):530-40
16022876
Respir Physiol Neurobiol. 2009 Nov 30;169(2):123-32
19698805
Exp Neurol. 1987 Jun;96(3):621-34
3446220
Exp Neurol. 1988 Aug;101(2):208-20
3396639
Exp Brain Res. 1996 Nov;112(1):35-40
8951404
Respir Physiol Neurobiol. 2007 Nov 15;159(2):115-26
17660051
J Physiol. 2007 Mar 15;579(Pt 3):863-76
17204503
Curr Opin Neurobiol. 2009 Dec;19(6):572-86
19896834
Trends Neurosci. 2008 Oct;31(10):538-47
18775573
Exp Brain Res. 1996 Jul;110(2):196-204
8836684
J Appl Physiol (1985). 2010 Jul;109(1):87-94
20395542
J Anat. 1978 Feb;125(Pt 2):377-86
624684
Brain Res Bull. 2002 Feb-Mar 1;57(3-4):335-9
11922984
J Comp Neurol. 1994 Sep 1;347(1):64-86
7798382
J Comp Neurol. 1997 Sep 15;386(1):2-7
9303520
J Appl Physiol (1985). 2000 Aug;89(2):563-72
10926639
Proc Natl Acad Sci U S A. 2009 Jun 9;106(23):9419-24
19474295