A fluorescent, paramagnetic and PEGylated gold/silica nanoparticle for MRI, CT and fluorescence imaging.
An important challenge in medical diagnostics is to design all-in-one contrast agents that can be detected with multiple techniques such as magnetic resonance imaging (MRI), X-ray computed tomography (CT), positron emission tomography (PET), single photon emission tomography (SPECT) or fluorescence imaging (FI). Although many dual labeled agents have been proposed, mainly for combined MRI/FI, constructs for three imaging modalities are scarce. Here gold/silica nanoparticles with a poly(ethylene glycol), paramagnetic and fluorescent lipid coating were synthesized, characterized and applied as trimodal contrast agents to allow for nanoparticle-enhanced imaging of macrophage cells in vitro via MRI, CT and FI, and mice livers in vivo via MRI and CT. This agent can be a useful tool in a multitude of applications, including cell tracking and target-specific molecular imaging, and is a step in the direction of truly multi-modal imaging.
van Schooneveld
Matti M
MM
Condensed Matter and Interfaces, Utrecht University, P.O. Box 80000, 3508 TA Utrecht, The Netherlands.
Cormode
David P
DP
Koole
Rolf
R
van Wijngaarden
J Timon
JT
Calcagno
Claudia
C
Skajaa
Torjus
T
Hilhorst
Jan
J
't Hart
Dannis C
DC
Fayad
Zahi A
ZA
Mulder
Willem J M
WJ
Meijerink
Andries
A
D000818:Animals; D003287:Contrast Media / Q000737:chemistry*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D006046:Gold / Q000737:chemistry; D008279:Magnetic Resonance Imaging / Q000379:methods*; D051379:Mice; D008810:Mice, Inbred C57BL; D053758:Nanoparticles / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry; D014057:Tomography, X-Ray Computed / Q000379:methods*
D003287:Contrast Media; D005456:Fluorescent Dyes; D011092:Polyethylene Glycols; D006046:Gold; D012822:Silicon Dioxide
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