Chemical characterization of Lycium barbarum polysaccharides and their reducing myocardial injury in ischemia/reperfusion of rat heart.
Polysaccharides were extracted from Lycium barbarum fruits in this work. Fourier transform infrared spectroscopy (FT-IR) and high-performance liquid chromatography (HPLC) have been employed to characterize this polysaccharides in the present study. The results of chemical composition indicated that the L. barbarum polysaccharides were composed of two kinds of monosaccharides, namely glucose and fructose in molar ratios of 1:2.1. The results indicated that the glucose and fructose were the predominant monosaccharides. IR spectrum of L. barbarum polysaccharides revealed a typical peaks of polysaccharides. The results still showed that L. barbarum polysaccharides significantly decreased the myocardium LD level, increased Na(+)-K(+)-ATPase and Ca(2+)-ATPase activities in heart ischemia reperfusion (IR) rats. In addition, L. barbarum polysaccharides still markedly decreased myocardium Bax positive rate and myocardial cell apoptosis and increased Bcl-2 positive rate in a dose-dependent manner. It may be concluded that administration of L. barbarum polysaccharides can prevented the development of cardiovascular diseases.
Lu
Shao-Ping
SP
Department of Cardiology, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, PR China. xjtulushaoping@yahoo.com.cn
Zhao
Pin-Ting
PT
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000494:pharmacology / Q000627:therapeutic use*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D015428:Myocardial Reperfusion Injury / Q000188:drug therapy* / Q000201:enzymology; D009206:Myocardium / Q000201:enzymology / Q000473:pathology; D009569:Nitric Oxide / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared; D051028:bcl-2-Associated X Protein / Q000378:metabolism
D004365:Drugs, Chinese Herbal; D051028:bcl-2-Associated X Protein; C500223:lycium barbarum polysaccharide; D009569:Nitric Oxide; D007770:L-Lactate Dehydrogenase; D000252:Calcium-Transporting ATPases; D000254:Sodium-Potassium-Exchanging ATPase