[An extracellular pH changes registration by confocal microscopy in higher plant at the excitation potentials generation].
Confocal microscopy technique was applied for registration of apoplast pH changes in the Cucurbita pepo seedling stem during generation of action potential and variation potential. Fluorescent dye FITC-dextran was used for pH-changes registration. Analysis of fluorescence images and fluorescence spectra showed, that FITC-dextran was localized in cell walls. Propagation of action potential and variation potential was accompanied by transient increase of fluorescence intensity, which indicated alkalization of cell walls. This transient alkalization is proposed to be caused by a temporary inactivation of the H(+)-pump in the plasma membrane.
Vodeneev
V A
VA
Akinchits
E K
EK
Orlova
L A
LA
Sukhov
V S
VS
Balalaeva
I V
IV
D000200:Action Potentials; D028464:Cucurbita / Q000502:physiology*; D005110:Extracellular Space / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D018613:Microscopy, Confocal / Q000379:methods; D013050:Spectrometry, Fluorescence