Selecting genetic transformants of indica and indica-derived rice cultivars using bispyribac sodium and a mutated ALS gene.
Bispyribac sodium (BS), a pyrimidinyl carboxy herbicide, is a well-known inhibitor of acetolactate synthase (ALS) activity. ALS is an enzyme in the biosynthetic pathway for branched-chain amino acids. A mutant form of rice ALS (OsmALS [W548L/S627I]) that confers resistance to BS can be used as an in vitro selection marker gene for plant transformation. Since indica and indica-derived cultivars are thought to have lower BS sensitivity than japonica rice, the application of BS as a selectable reagent for genetic transformation in indica and indica-derived cultivars is more challenging than for japonica cultivars. In this study, callus and seedlings of eight different rice cultivars (five indica-derived cultivars, two indica cultivars and one japonica cultivar) were tested for BS sensitivity. Our study indicates for the first time that callus shows a higher sensitivity to BS than seedlings in indica and indica-derived cultivars. We used BS with OsmALS [W548L/S627I] to select transformed calli, and transgenic rice plants from indica and indica-derived cultivars were successfully obtained.
Taniguchi
Yojiro
Y
National Institute of Crop Science, Kannondai 2-1-18, Tsukuba, Ibaraki, 305-8518, Japan.
Kawata
Motoshige
M
Ando
Ikuo
I
Shimizu
Tsutomu
T
Ohshima
Masahiro
M
D000095:Acetolactate Synthase / Q000235:genetics* / Q000378:metabolism; D001565:Benzoates / Q000494:pharmacology*; D005822:Genetic Vectors; D006540:Herbicides / Q000494:pharmacology*; D012275:Oryza / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D011743:Pyrimidines / Q000494:pharmacology*; D036226:Seedlings / Q000187:drug effects; D014170:Transformation, Genetic
D001565:Benzoates; D006540:Herbicides; D011743:Pyrimidines; C549011:bispyribac; D000095:Acetolactate Synthase
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