Identification of novel immunogenic proteins in pathogenic Haemophilus parasuis based on genome sequence analysis.
Haemophilus parasuis causes contagious porcine Glässer's disease, which is occurring worldwide and leads to severe losses in the pig industry. To identify novel antigen candidates against this disease, 22 surface-exposed or secreted proteins were selected from the annotated H. parasuis genome by reverse vaccinology strategy. Expression of these proteins in Escherichia coli was attempted. Immunogenicity of the expressed candidates was assessed using Western blot analysis with mouse-derived antiserum prepared with whole bacteria of H. parasuis serovar 4 or 5. Three ABC-type transporters (OppA, YfeA and PlpA) and 1 curli protein assembly (CsgG) were identified as potent immunogenic proteins. The proteins show cross-reactions when tested with sera raised against serovars 4 and 5 of H. parasuis.
Hong
Minhee
M
Biotechnology Process Engineering Center, KRIBB, Daejeon 305-600, Republic of Korea.
Ahn
Jungoh
J
Yoo
Sungsik
S
Hong
Jiyeon
J
Lee
Eungyu
E
Yoon
Injoong
I
Jung
Joon-ki
JK
Lee
Hongweon
H
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics* / Q000276:immunology; D003429:Cross Reactions; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D044137:Haemophilus parasuis / Q000235:genetics* / Q000276:immunology; D007106:Immune Sera / Q000276:immunology; D051379:Mice
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D007106:Immune Sera