A new crystal form of Lys48-linked diubiquitin.
Lys48-linked polyubiquitin chains are recognized by the proteasome as a tag for the degradation of the attached substrates. Here, a new crystal form of Lys48-linked diubiquitin (Ub2) was obtained and the crystal structure was refined to 1.6 A resolution. The structure reveals an ordered isopeptide bond in a trans configuration. All three molecules in the asymmetric unit were in the same closed conformation, in which the hydrophobic patches of both the distal and the proximal moieties interact with each other. Despite the different crystallization conditions and different crystal packing, the new crystal structure of Ub2 is similar to the previously published structure of diubiquitin, but differences are observed in the conformation of the flexible isopeptide linkage.
Trempe
Jean François
JF
Department of Biochemistry, McGill University, 3649 Promenade Sir William Osler, Montreal, Québec H3G 0B1, Canada. jean.trempe@mail.mcgill.ca
Brown
Nicholas R
NR
Noble
Martin E M
ME
Endicott
Jane A
JA
D018360:Crystallography, X-Ray; D006801:Humans; D008239:Lysine / Q000737:chemistry; D008958:Models, Molecular; D020836:Protein Structure, Quaternary; D025801:Ubiquitin / Q000737:chemistry*
D025801:Ubiquitin; D008239:Lysine
Nat Cell Biol. 2001 Oct;3(10):939-43
11584278
Nat Rev Mol Cell Biol. 2005 Aug;6(8):610-21
16064137
J Biomol NMR. 2006 Feb;34(2):101-15
16518697
Mol Cell. 2009 Aug 14;35(3):280-90
19683493
Nature. 2008 May 22;453(7194):548-52
18497827
Acta Crystallogr D Biol Crystallogr. 1997 Jul 1;53(Pt 4):448-55
15299911
Nat Struct Biol. 2001 May;8(5):417-22
11323716
Structure. 1996 Dec 15;4(12):1395-400
8994966
Acta Crystallogr D Biol Crystallogr. 2001 Feb;57(Pt 2):341-4
11173499
Proteins. 2006 Jun 1;63(4):787-96
16609980
J Biol Chem. 2002 Jan 4;277(1):516-27
11675391
EMBO J. 2005 Sep 21;24(18):3178-89
16138082
Mol Cell. 2009 Dec 25;36(6):1018-33
20064467
Acta Crystallogr D Biol Crystallogr. 2006 Jan;62(Pt 1):72-82
16369096
Acta Crystallogr D Biol Crystallogr. 1997 May 1;53(Pt 3):240-55
15299926
J Biol Chem. 1992 Aug 15;267(23):16467-71
1322903
J Mol Biol. 1987 Apr 5;194(3):531-44
3041007
Acta Crystallogr D Biol Crystallogr. 1999 Jan;55(Pt 1):191-205
10089410
Mol Cell. 2005 Jun 10;18(6):687-98
15949443
J Mol Biol. 2002 Dec 6;324(4):637-47
12460567
J Biol Chem. 1994 Mar 11;269(10):7059-61
8125911
J Biol Chem. 1990 Dec 15;265(35):21835-42
2174887
Proteins. 2005 Aug 15;60(3):367-81
15937902
Acta Crystallogr D Biol Crystallogr. 2006 Jan;62(Pt 1):48-57
16369093
Acta Crystallogr D Biol Crystallogr. 2004 Dec;60(Pt 12 Pt 1):2126-32
15572765
Nature. 2008 May 22;453(7194):481-8
18497817
J Mol Biol. 1994 Feb 18;236(2):601-9
8107144
J Appl Crystallogr. 2007 Aug 1;40(Pt 4):658-674
19461840
J Biol Chem. 1997 Sep 19;272(38):23712-21
9295315
Annu Rev Biochem. 2009;78:477-513
19489727
Annu Rev Biochem. 1998;67:425-79
9759494