title: Nuclear magnetic resonance (NMR) study of the effect of cisplatin on the metabolic profile of MG-63 osteosarcoma cells.
abstract: In the present study, (1)H HRMAS NMR spectroscopy was used to assess the changes in the intracellular metabolic profile of MG-63 human osteosarcoma (OS) cells induced by the chemotherapy agent cisplatin (CDDP) at different times of exposure. Multivariate analysis was applied to the cells spectra, enabling consistent variation patterns to be detected and drug-specific metabolic effects to be identified. Statistical recoupling of variables (SRV) analysis and spectral integration enabled the most relevant spectral changes to be evaluated, revealing significant time-dependent alterations in lipids, choline-containing compounds, some amino acids, polyalcohols, and nitrogenated bases. The metabolic relevance of these compounds in the response of MG-63 cells to CDDP treatment is discussed.
authors.affiliation: CICECO-Departmento de Química, Campus Universitário de Santiago, Universidade de Aveiro, 3810-193 Aveiro, Portugal.
authors.forename: Iola F
authors.initials: IF
authors.lastname: Duarte
authors.forename: Ines
authors.initials: I
authors.lastname: Lamego
authors.forename: Joana
authors.initials: J
authors.lastname: Marques
authors.forename: M Paula M
authors.initials: MP
authors.lastname: Marques
authors.forename: Benjamin J
authors.initials: BJ
authors.lastname: Blaise
authors.forename: Ana M
authors.initials: AM
authors.lastname: Gil
mesh_terms: D000438:Alcohols; D000596:Amino Acids; D002794:Choline; D002945:Cisplatin / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics; D008055:Lipids; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D055442:Metabolome / Q000187:drug effects*; D012516:Osteosarcoma / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology
chemical_list: D000438:Alcohols; D000596:Amino Acids; D008055:Lipids; D002794:Choline; D002945:Cisplatin