Identification of ptpro as a novel target gene of Wnt signaling and its potential role as a receptor for Wnt.
Wnt/β-catenin signaling plays critical roles in embryonic development and tissue homeostasis in adults by controlling the expression of target genes. We found that expression of ptpro, which encodes a protein tyrosine phosphatase receptor type O (PTPRO), was induced by Wnt/β-catenin signaling in a T cell factor/lymphoid enhancer factor dependent manner. Biochemical assays found that PTPRO interacted with Wnt via its extracellular domain. In addition, ectopic expression of this extracellular domain inhibited Wnt-mediated reporter activity. These results suggest that ptpro is a target gene of Wnt/β-catenin signaling and that PTPRO may function as a novel receptor for Wnt.
Kim
Minseong
M
Department of Life Science, The University of Seoul, Seoul, Republic of Korea.
Kim
Hanjun
H
Jho
Eek-hoon
EH
D000818:Animals; D002460:Cell Line; D045744:Cell Line, Tumor; D005786:Gene Expression Regulation; D006801:Humans; D051821:Lymphoid Enhancer-Binding Factor 1 / Q000378:metabolism; D051379:Mice; D054631:Receptor-Like Protein Tyrosine Phosphatases, Class 3 / Q000235:genetics* / Q000378:metabolism*; D015398:Signal Transduction; D051820:TCF Transcription Factors / Q000378:metabolism; D051153:Wnt Proteins / Q000378:metabolism*; D051176:beta Catenin / Q000378:metabolism
C497574:Lef1 protein, mouse; D051821:Lymphoid Enhancer-Binding Factor 1; D051820:TCF Transcription Factors; D051153:Wnt Proteins; D051176:beta Catenin; C516890:Ptpro protein, mouse; D054631:Receptor-Like Protein Tyrosine Phosphatases, Class 3