On the use of 2,1,3-benzothiadiazole derivatives as selective live cell fluorescence imaging probes.
Newly designed 2,1,3-benzothiadiazole-containing fluorescent probes with four excited state intramolecular proton transfer (ESIPT) sites were successfully tested in live cell-imaging assays using a confluent monolayer of human stem-cells (tissue). All tested dyes were compared with the commercially available DAPI and gave far better results.
Oliveira
Felipe F D
FF
Laboratory of Medicinal and Technological Chemistry, University of Brasília (IQ-UnB), Brasília, DF, Brazil.
Santos
Diego C B D
DC
Lapis
Alexandre A M
AA
Corrêa
José R
JR
Gomes
Alexandre F
AF
Gozzo
Fabio C
FC
Moreira
Paulo F
PF
de Oliveira
Virgínia C
VC
Quina
Frank H
FH
Neto
Brenno A D
BA
D002470:Cell Survival; D002478:Cells, Cultured; D004277:DNA, Single-Stranded / Q000032:analysis; D005456:Fluorescent Dyes / Q000032:analysis* / Q000737:chemistry; D006801:Humans; D013050:Spectrometry, Fluorescence; D013234:Stem Cells / Q000166:cytology*; D013830:Thiadiazoles / Q000032:analysis* / Q000737:chemistry
D004277:DNA, Single-Stranded; D005456:Fluorescent Dyes; D013830:Thiadiazoles; C015700:benzo-1,2,3-thiadiazole