Clinical evaluation of Bio-Rad MRSASelect™ medium for the detection of livestock-associated methicillin-resistant Staphylococcus aureus.
Bio-Rad MRSASelect™ medium was evaluated for its ability to recover methicillin-resistant Staphylococcus aureus (MRSA) from nasal samples of pig farmers and their household members. In total, 257 samples were inoculated on Bio-Rad MRSASelect™ medium with and without broth enrichment and on bioMérieux MRSA ID with broth enrichment. A sample was considered to be positive if at least one of the media grew MRSA. The sensitivity of Bio-Rad MRSASelect™ medium without broth enrichment was 63.9%. With broth enrichment, the sensitivity increased to 98.4%. The specificity was 95.4% both with and without broth enrichment. In conclusion, Bio-Rad MRSASelect™ medium as well as MRSA ID medium are reliable methods to detect MRSA carriage when used in combination with broth enrichment. The directly inoculated MRSASelect™ medium was statistically significantly less sensitive than the two media after broth enrichment.
Verkade
E
E
St. Elisabeth Hospital, Tilburg, The Netherlands. everkade@amphia.nl
Verhulst
C
C
van Cleef
B
B
Kluytmans
J
J
D000383:Agriculture; D000818:Animals; D001431:Bacteriological Techniques / Q000379:methods*; D002353:Carrier State / Q000175:diagnosis* / Q000382:microbiology; D003470:Culture Media / Q000737:chemistry*; D005192:Family Health; D006801:Humans; D058751:Livestock; D055624:Methicillin-Resistant Staphylococcus aureus / Q000302:isolation & purification*; D009297:Nasal Mucosa / Q000382:microbiology; D012680:Sensitivity and Specificity; D013203:Staphylococcal Infections / Q000175:diagnosis* / Q000382:microbiology
D003470:Culture Media
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