Structural studies of MAP Kinase cascade components.
MAPK cascade components have been the subject of structural analysis, advancing our understanding of how these enzymes are activated and how they interact. A surprising finding has been that unique inactive conformers are adopted by many of these kinases. These inactive conformers are interesting and often require experimental phases to determine their crystal structures because molecular replacement techniques are not successful. Here, we describe the preparation of MAP2K MEK6 and MAP3K TAO2 substituted with selenomethionine (SeMet) for de novo phasing. TAO2 and SeMet TAO2 were expressed in insect cells.
Goldsmith
Elizabeth J
EJ
Department of Biochemistry, The University of Texas Southwestern Medical Center at Dallas, Dallas, TX, USA. Elizabeth.Goldsmith@UTSouthwestern.edu
Min
Xiaoshan
X
He
Haixia
H
Zhou
Tianjun
T
D000818:Animals; D002460:Cell Line; D003460:Crystallization; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D048669:MAP Kinase Kinase 6 / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D020930:MAP Kinase Kinase Kinases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D020935:MAP Kinase Signaling System; D051379:Mice; D010766:Phosphorylation; D051381:Rats; D012645:Selenomethionine / Q000378:metabolism
D012645:Selenomethionine; D020930:MAP Kinase Kinase Kinases; D048669:MAP Kinase Kinase 6