Role of epicellular molecules in the selection of particles by the blue mussel, Mytilus edulis.
This study provides evidence that the suspension-feeding blue mussel, Mytilus edulis, uses biochemical cues to recognize its food. We identified lectins in mucus from the gills and labial palps, two pallial organs involved in the feeding process. These compounds were able to agglutinate rabbit and horse erythrocytes (RBC) and several species of marine microalgae representing different families. Additionally, the agglutination of RBC and microalgae was inhibited by several carbohydrates (fetuin, lipopolysaccharide (LPS), and mannose-related residues), suggesting that a suite of lectins may be present in mucus from the gills and labial palps. Results from feeding experiments, using microspheres with tailored surfaces, demonstrated that mussels preferentially ingested microspheres coated with the neoglycoproteins glucosamide-BSA and mannopyranosylphenyl-BSA but rejected in pseudofeces microspheres coated with BSA alone. The positive selection for neoglycoprotein-coated microspheres was inhibited when mussels were pre-incubated in seawater containing a solution of the same neoglycoprotein. Two surface properties of the microspheres, charge and wettability, had little effect on the observed selection process. Our results, along with our previous findings for oysters, suggest a new concept for the mechanism of particle selection in bivalves and perhaps other suspension-feeding organisms. Specifically, the selection of particles involves interactions between epiparticulate carbohydrates and lectins in the mucus produced by feeding organs.
Espinosa
Emmanuelle Pales
EP
School of Marine and Atmospheric Sciences, State University of New York, Stony Brook, New York 11794, USA. Emmanuelle.Palesespinosa@stonybrook.edu
Hassan
Dahlia
D
Ward
J Evan
JE
Shumway
Sandra E
SE
Allam
Bassem
B
D000818:Animals; D050260:Carbohydrate Metabolism; D044785:Cryptophyta; D017377:Diatoms; D056890:Eukaryota; D005247:Feeding Behavior; D037102:Lectins / Q000378:metabolism / Q000502:physiology*; D009093:Mucus / Q000737:chemistry / Q000502:physiology*; D049880:Mytilus edulis / Q000737:chemistry / Q000502:physiology*; D052638:Particulate Matter; D011485:Protein Binding
D037102:Lectins; D052638:Particulate Matter