Difference and variation of the sef14 operon gene clusters in Salmonella pullorum.
SEF14 fimbriae are only found in some strains of serogroup-D Salmonella such as S. enteritidis, suggesting that SEF14 fimbriae may affect serovar-specific virulence traits. In this study, we found that prevalence of sefA, sefD and sefR genes in S. dublin and S. enteritidis was 100%. In 18 isolates of S. pullorum, the prevalence of sefA gene was 100%, while the prevalence of sefD and sefR genes was 38.9% (7/18), and 11 strains isolated after 1980s did not contain any gene sefD or sefR. Interestingly, among the 7 strains of S. pullorum before 1980s, the sefD sequence has a missing base pair at position 196 and caused open reading frame (ORF) shift, resulting in a stop codon (TAG) at position 71 amino acid residual (Leu of TTA at position 214-216 shift into stop codon of TAG at position 215-217). Unlike S. pullorum, all S. enteritidis and S. dublin tested could express SEF14 fimbriae in vitro.
Zhu
C H
CH
College of Veterinary Medicine, Yangzhou University, Yangzhou, China.
Wu
J
J
Chen
W W
WW
Hassan
H Musa
HM
Zhu
G Q
GQ
D001426:Bacterial Proteins / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D036781:Fimbriae Proteins / Q000235:genetics*; D005798:Genes, Bacterial; D005810:Multigene Family; D016366:Open Reading Frames; D009876:Operon; D012475:Salmonella / Q000235:genetics*
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D036781:Fimbriae Proteins