Pyramidal neurons are generated from oligodendroglial progenitor cells in adult piriform cortex.
Previous studies have shown that oligodendroglial progenitor cells (OPCs) can give rise to neurons in vitro and in perinatal cerebral cortex in vivo. We now report that OPCs in adult murine piriform cortex express low levels of doublecortin, a marker for migratory and immature neurons. Additionally, these OPCs express Sox2, a neural stem cell marker, and Pax6, a transcription factor characteristic of progenitors for cortical glutamatergic neurons. Genetic fate-mapping by means of an inducible Cre-LoxP recombination system proved that these OPCs differentiate into pyramidal glutamatergic neurons in piriform cortex. Several lines of evidence indicated that these newly formed neurons became functionally integrated into the cortical neuronal network. Our data suggest that NG2(+)/PDGFRα(+) proteolipid protein promoter-expressing progenitors generate pyramidal glutamatergic neurons within normal adult piriform cortex.
Guo
Fuzheng
F
Institute for Pediatric Regenerative Medicine, University of California, Davis School of Medicine, Sacramento, California 95817, USA.
Maeda
Yoshiko
Y
Ma
Joyce
J
Xu
Jie
J
Horiuchi
Makoto
M
Miers
Laird
L
Vaccarino
Flora
F
Pleasure
David
D
D053687:Adult Stem Cells / Q000187:drug effects / Q000502:physiology*; D000818:Animals; D000941:Antigens / Q000378:metabolism; D018931:Antineoplastic Agents, Hormonal / Q000494:pharmacology; D001973:Bromodeoxyuridine / Q000378:metabolism; D002452:Cell Count / Q000379:methods; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D002540:Cerebral Cortex / Q000166:cytology*; D000089622:Doublecortin Domain Proteins; D004334:Drug Administration Schedule; D005136:Eye Proteins / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000235:genetics; D018398:Homeodomain Proteins / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D018991:Myelin Proteolipid Protein / Q000235:genetics; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009474:Neurons / Q000502:physiology; D009479:Neuropeptides / Q000378:metabolism; D009836:Oligodendroglia / Q000502:physiology*; D000071841:PAX6 Transcription Factor; D051761:Paired Box Transcription Factors / Q000378:metabolism; D011509:Proteoglycans / Q000378:metabolism; D017966:Pyramidal Cells / Q000502:physiology*; D020796:Receptor, Platelet-Derived Growth Factor alpha / Q000378:metabolism; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism; D012097:Repressor Proteins / Q000378:metabolism; D055748:SOXB1 Transcription Factors / Q000378:metabolism; D013629:Tamoxifen / Q000494:pharmacology; D013997:Time Factors; D000099216:Chondroitin Sulfate Proteoglycan 4
D000941:Antigens; D018931:Antineoplastic Agents, Hormonal; D001973:Bromodeoxyuridine; D000089622:Doublecortin Domain Proteins; D005136:Eye Proteins; D049452:Green Fluorescent Proteins; D018398:Homeodomain Proteins; D008869:Microtubule-Associated Proteins; D018991:Myelin Proteolipid Protein; D009419:Nerve Tissue Proteins; D009479:Neuropeptides; D000071841:PAX6 Transcription Factor; D051761:Paired Box Transcription Factors; D011509:Proteoglycans; D020796:Receptor, Platelet-Derived Growth Factor alpha; D016194:Receptors, N-Methyl-D-Aspartate; D012097:Repressor Proteins; D055748:SOXB1 Transcription Factors; D013629:Tamoxifen; D000099216:Chondroitin Sulfate Proteoglycan 4; C409466:NR1 NMDA receptor; C000605732:Pax6 protein, mouse; C047516:Plp1 protein, mouse; C527783:Sox2 protein, mouse; C485184:enhanced green fluorescent protein
Proc Natl Acad Sci U S A. 2002 Aug 20;99(17):11464-9
12177450
Nat Neurosci. 2008 Dec;11(12):1392-401
18849983
Genesis. 2003 Jan;35(1):63-72
12481300
Nat Neurosci. 2004 Nov;7(11):1233-41
15494728
J Neurosci. 2006 Aug 16;26(33):8609-21
16914687
Nature. 2000 May 11;405(6783):187-91
10821275
J Cell Biol. 2003 Apr 14;161(1):169-86
12682089
Brain Res. 2004 Nov 5;1026(1):84-94
15476700
J Neurosci. 2005 Nov 16;25(46):10729-39
16291946
J Neurosci. 1996 Apr 15;16(8):2659-70
8786442
Glia. 2010 Jun;58(8):943-53
20155815
Nat Neurosci. 2010 Mar;13(3):287-289
20173746
J Neurosci. 2006 Nov 15;26(46):11938-47
17108168
Cereb Cortex. 2008 Oct;18(10):2229-40
18245040
Aging Cell. 2005 Aug;4(4):197-207
16026334
Eur J Neurosci. 2007 Jun;25(12):3489-98
17610569
Chem Senses. 2001 Jun;26(5):551-76
11418502
Nature. 1983 Jun 2-8;303(5916):390-6
6304520
Cereb Cortex. 2009 May;19(5):1028-41
18832334
Mol Cell Neurosci. 2008 Aug;38(4):616-28
18595732
Brain Struct Funct. 2007 Sep;212(2):133-48
17764016
J Neurosci. 2008 Jul 23;28(30):7610-23
18650338
J Neurosci Res. 2004 May 1;76(3):282-95
15079857
Nature. 2000 Jun 22;405(6789):951-5
10879536
Nat Neurosci. 2010 Feb;13(2):173-9
20037576
J Neurosci. 2008 Jan 2;28(1):264-78
18171944
Development. 1998 Aug;125(15):2901-14
9655812
Nat Neurosci. 2008 Apr;11(4):450-6
18311136
J Neurosci. 2002 Feb 1;22(3):876-85
11826117
Science. 2000 Sep 8;289(5485):1754-7
10976069
Nat Rev Neurosci. 2007 Jun;8(6):481-8
17514200
Science. 1999 Oct 15;286(5439):548-52
10521353
J Physiol. 2004 Nov 15;561(Pt 1):109-22
15358811
Mol Cell Neurosci. 2003 Oct;24(2):476-88
14572468
J Comp Neurol. 2007 Nov 10;505(2):209-20
17853440
Cell Stem Cell. 2007 Nov;1(5):515-28
18371391
J Neurocytol. 1989 Jun;18(3):311-8
2746304
Development. 2008 Jan;135(1):145-57
18045844
J Neurosci. 2009 Jun 3;29(22):7256-70
19494148
J Neurosci. 1997 Oct 1;17(19):7372-84
9295383
Science. 2005 Oct 28;310(5748):679-83
16254185
J Neurosci. 2006 Nov 1;26(44):11413-22
17079670
J Neurosci. 2005 Jan 5;25(1):247-51
15634788
J Neurosci. 2004 Nov 17;24(46):10530-41
15548668
Nat Neurosci. 2009 Dec;12(12):1524-33
19881504
J Neurosci. 2009 Nov 25;29(47):14965-79
19940192
Neurobiol Aging. 2008 Jan;29(1):129-47
17092610
Proc Natl Acad Sci U S A. 2008 Dec 30;105(52):20994-9
19095802
BMC Dev Biol. 2001;1:4
11299042
Science. 2000 Feb 25;287(5457):1433-8
10688783
J Neurosci. 2008 Oct 8;28(41):10434-42
18842903
PLoS Biol. 2008 Nov 11;6(11):e272
18998770
J Neurosci. 2006 Jan 25;26(4):1269-74
16436614
J Neurosci. 2010 Mar 10;30(10):3600-11
20219994
J Cell Biol. 2004 May 24;165(4):575-89
15159421
J Neurosci. 2007 Apr 4;27(14):3734-42
17409237
J Neurosci Res. 2010 Apr;88(5):957-70
19908280
J Neurosci. 2006 Oct 25;26(43):10967-83
17065439
J Neurosci Res. 2009 Oct;87(13):2842-53
19479988
J Cell Biol. 2005 Jan 31;168(3):415-27
15684031
Nat Med. 2002 Sep;8(9):963-70
12161747
Glia. 1999 Mar;26(1):84-91
10088675