title: Studying the regulation of MAP Kinase by MAP Kinase phosphatases in vitro and in cell systems.
abstract: Signaling through MAPK pathways involves a network of activating kinases and inactivating phosphatases. While single MAPK kinases account for specific activation of the distinct MAPKs, inactivation of MAPKs by phosphatases involves a wider spectrum of enzymes, with phosphatases from distinct families displaying specificity toward MAPKs. The dual-specificity family of MAPK phosphatases, MKPs, constitutes the major group of MAPK inactivating phosphatases. MKPs are widely expressed, in a tissue- and development-regulated manner, and the control of their expression and function is crucial for the regulation of MAPK signaling. Here, we present three methods to analyze the regulation of MAPKs by MKPs, using transient and stable-inducible MKP overexpression cell systems and in vitro phosphatase experiments.
authors.affiliation: Centro de Investigación Príncipe Felipe, Valencia, Spain.
authors.forename: Céline
authors.initials: C
authors.lastname: Tárrega
authors.forename: Caroline
authors.initials: C
authors.lastname: Nunes-Xavier
authors.forename: Rocío
authors.initials: R
authors.lastname: Cejudo-Marín
authors.forename: Jorge
authors.initials: J
authors.lastname: Martín-Pérez
authors.forename: Rafael
authors.initials: R
authors.lastname: Pulido
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D057075:Enzyme Assays / Q000379:methods*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D006801:Humans; D054639:Mitogen-Activated Protein Kinase Phosphatases / Q000378:metabolism*; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D013379:Substrate Specificity; D013752:Tetracycline / Q000494:pharmacology
chemical_list: D020928:Mitogen-Activated Protein Kinases; D054639:Mitogen-Activated Protein Kinase Phosphatases; D013752:Tetracycline