title: Inhibitory effects of zingerone, a pungent component of Zingiber officinale Roscoe, on colonic motility in rats.
abstract: Ginger (rhizome of Zingiber officinale Roscoe) is an herbal medicine for the treatment of gastrointestinal disorders including constipation and diarrhea. Zingerone is a likely active constituent responsible for the antidiarrheal activity of ginger. The current study was designed to characterize pharmacological actions of zingerone on colonic motility. To evaluate pharmacological effects of zingerone on colonic motility, we used isolated colonic segments from rats, in which mechanical responses were recorded in the longitudinal direction. In addition, we evaluated the effects on colonic motility in vivo by measuring intraluminal pressure changes and expelled fluid volume from the colon in anesthetized rats. Zingerone was applied to the lumen of the colon to allow the drug to access from the mucosal side. Zingerone inhibited spontaneous contractile movements in the isolated colonic segments in a dose-dependent manner. The inhibitory effects of zingerone on colonic movements were not affected by pretreatment with capsazepine, a typical antagonist of transient receptor potential vanilloid 1. In addition, tetrodotoxin, a blocker of voltage-dependent sodium channels on neurons, did not affect the suppression of colonic movements by zingerone, suggesting that zingerone acts on the smooth muscles directly. Zingerone also attenuated colonic motility in vivo without affecting blood pressure and heart rate. The effects were reversible and reproducible. Our findings suggest that zingerone can inhibit colonic motility via direct action on smooth muscles. Zingerone might exert beneficial therapeutic effects on hypermotility-induced diarrhea by abrogating excessive gastrointestinal motility.
authors.affiliation: Laboratory of Physiology, Department of Basic Veterinary Science, The United Graduate School of Veterinary Sciences, 1-1 Yanagido, Gifu 501-1193, Japan.
authors.forename: Momoe
authors.initials: M
authors.lastname: Iwami
authors.forename: Takahiko
authors.initials: T
authors.lastname: Shiina
authors.forename: Haruko
authors.initials: H
authors.lastname: Hirayama
authors.forename: Takeshi
authors.initials: T
authors.lastname: Shima
authors.forename: Tadashi
authors.initials: T
authors.lastname: Takewaki
authors.forename: Yasutake
authors.initials: Y
authors.lastname: Shimizu
mesh_terms: D000818:Animals; D003106:Colon / Q000187:drug effects*; D005769:Gastrointestinal Motility / Q000187:drug effects*; D020939:Zingiber officinale / Q000737:chemistry*; D006139:Guaiacol / Q000031:analogs & derivatives* / Q000494:pharmacology; D066298:In Vitro Techniques; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar
chemical_list: C013738:zingerone; D006139:Guaiacol
references.citation: Pharmacol Rev. 1999 Jun;51(2):159-212
references.pmid: 10353985
references.citation: Pharmacology. 1999 Oct;59(4):212-20
references.pmid: 10474081
references.citation: Am J Chin Med. 2001;29(1):111-8
references.pmid: 11321468
references.citation: Biol Pharm Bull. 2004 Jul;27(7):1046-51
references.pmid: 15256738
references.citation: Life Sci. 2001 Apr 13;68(21):2347-60
references.pmid: 11350006
references.citation: Am J Physiol Gastrointest Liver Physiol. 2001 May;280(5):G949-57
references.pmid: 11292604
references.citation: Br J Pharmacol. 2003 Sep;140(2):231-8
references.pmid: 12970093
references.citation: J Ethnopharmacol. 2006 Oct 11;107(3):342-8
references.pmid: 16713155
references.citation: Neurogastroenterol Motil. 2004 Dec;16(6):701-11
references.pmid: 15601419
references.citation: Eur J Pharmacol. 1979 Apr 15;55(2):181-7
references.pmid: 456415
references.citation: J Agric Food Chem. 2007 Oct 17;55(21):8390-7
references.pmid: 17880155
references.citation: Eur J Pharmacol. 2006 Jan 13;530(1-2):144-51
references.pmid: 16360146
references.citation: Am J Physiol. 1986 Jan;250(1 Pt 1):G1-8
references.pmid: 2867681
references.citation: J Ethnopharmacol. 1989 Nov;27(1-2):129-40
references.pmid: 2615416
references.citation: Fitoterapia. 2007 Apr;78(3):171-4
references.pmid: 17275209
references.citation: Physiol Rev. 2002 Jan;82(1):245-89
references.pmid: 11773614
references.citation: Eur J Pharmacol. 1990 Sep 4;186(1):71-7
references.pmid: 1704311
references.citation: Crit Rev Food Sci Nutr. 1982;17(1):1-96
references.pmid: 7049579
references.citation: Food Chem Toxicol. 2008 Feb;46(2):409-20
references.pmid: 17950516
references.citation: J Physiol Paris. 2001 Jan-Dec;95(1-6):181-8
references.pmid: 11595435
references.citation: Gut. 1991 Nov;32(11):1355-9
references.pmid: 1752468
references.citation: Eur J Pharmacol. 2004 Mar 8;487(1-3):175-82
references.pmid: 15033390
references.citation: J Ethnopharmacol. 1996 Sep;53(3):157-63
references.pmid: 8887023
references.citation: Biol Pharm Bull. 2001 Jun;24(6):623-7
references.pmid: 11411548
references.citation: Adv Ther. 1998 Jan-Feb;15(1):25-44
references.pmid: 10178636
references.citation: Eur J Biochem. 1998 Jan 15;251(1-2):262-8
references.pmid: 9492292
references.citation: Curr Neuropharmacol. 2008 Mar;6(1):79-96
references.pmid: 19305789
references.citation: Dig Dis Sci. 2005 Oct;50(10):1889-97
references.pmid: 16187193
references.citation: Food Chem Toxicol. 2007 May;45(5):683-90
references.pmid: 17175086
references.citation: J Neurophysiol. 1996 Sep;76(3):1858-69
references.pmid: 8890298
references.citation: Life Sci. 2004 Apr 23;74(23):2889-96
references.pmid: 15050426
references.citation: Regul Pept. 2009 Jun 5;155(1-3):11-7
references.pmid: 19345246
references.citation: Aliment Pharmacol Ther. 2001 Sep;15(9):1239-52
references.pmid: 11552894
references.citation: Physiol Behav. 2000 Apr 1-15;69(1-2):177-86
references.pmid: 10854928
references.citation: Pharmacol Rev. 2005 Sep;57(3):315-38
references.pmid: 16109838
references.citation: Am J Physiol Gastrointest Liver Physiol. 2009 Aug;297(2):G348-60
references.pmid: 19497956