Ferricytochrome c protects mitochondrial cytochrome c oxidase against hydrogen peroxide-induced oxidative damage.
An excess of ferricytochrome c protects purified mitochondrial cytochrome c oxidase and bound cardiolipin from hydrogen peroxide-induced oxidative modification. All of the peroxide-induced changes within cytochrome c oxidase, such as oxidation of Trp(19,IV) and Trp(48,VIIc), partial dissociation of subunits VIa and VIIa, and generation of cardiolipin hydroperoxide, no longer take place in the presence of ferricytochrome c. Furthermore, ferricytochrome c suppresses the yield of H(2)O(2)-induced free radical detectable by electron paramagnetic resonance spectroscopy within cytochrome c oxidase. These protective effects are based on two mechanisms. The first involves the peroxidase/catalase-like activity of ferricytochrome c, which results in the decomposition of H(2)O(2), with the apparent bimolecular rate constant of 5.1±1.0M(-1)s(-1). Although this value is lower than the rate constant of a specialized peroxidase, the activity is sufficient to eliminate H(2)O(2)-induced damage to cytochrome c oxidase in the presence of an excess of ferricytochrome c. The second mechanism involves ferricytochrome c-induced quenching of free radicals generated within cytochrome c oxidase. These results suggest that ferricytochrome c may have an important role in protection of cytochrome c oxidase and consequently the mitochondrion against oxidative damage.
Sedlák
Erik
E
Department of Biochemistry, The University of Texas Health Science Center, San Antonio, TX 78229-3900, USA.
Fabian
Marian
M
Robinson
Neal C
NC
Musatov
Andrej
A
D000818:Animals; D002308:Cardiolipins / Q000378:metabolism; D002417:Cattle; D045304:Cytochromes c / Q000737:chemistry* / Q000494:pharmacology; D004578:Electron Spin Resonance Spectroscopy; D003576:Electron Transport Complex IV / Q000737:chemistry* / Q000378:metabolism; D005609:Free Radicals / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000737:chemistry* / Q000378:metabolism; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D009206:Myocardium / Q000201:enzymology; D010084:Oxidation-Reduction
D002308:Cardiolipins; D005609:Free Radicals; D045304:Cytochromes c; D006861:Hydrogen Peroxide; D003576:Electron Transport Complex IV
Can J Biochem Physiol. 1959 Aug;37(8):911-7
13671378
Biochim Biophys Acta. 2002 Jul 1;1554(3):137-46
12160986
Biochem J. 1980 Nov 1;191(2):421-7
6263247
Biochemistry. 2006 Apr 18;45(15):4998-5009
16605268
Biochim Biophys Acta. 1991 Mar 8;1077(1):86-90
1849014
Biochemistry. 2002 Jun 25;41(25):8212-20
12069614
Arch Biochem Biophys. 2003 Dec 15;420(2):287-97
14654068
FEBS Lett. 1992 Feb 3;297(1-2):63-6
1312951
Methods Enzymol. 1978;52:302-10
672633
Nat Chem Biol. 2005 Sep;1(4):223-32
16408039
J Biol Chem. 1990 Sep 25;265(27):16330-6
2168888
Mech Ageing Dev. 2006 Mar;127(3):298-306
16405961
J Biol Chem. 1987 Jan 15;262(2):595-604
3027057
Cell. 1996 Jul 12;86(1):147-57
8689682
Biochemistry. 1995 Oct 24;34(42):13802-10
7577973
Biochemistry. 1999 Dec 7;38(49):16236-45
10587446
Biochem Biophys Res Commun. 2004 May 14;317(4):980-7
15094365
J Am Soc Mass Spectrom. 2006 Nov;17(11):1518-25
16872833
Arch Biochem Biophys. 1985 Mar;237(2):408-14
2983613
Proc Natl Acad Sci U S A. 2006 Jun 13;103(24):8965-70
16757556
Biochem Soc Trans. 2003 Dec;31(Pt 6):1312-5
14641051
J Biol Chem. 1998 May 22;273(21):12863-9
9582316
Ann N Y Acad Sci. 2000;899:112-20
10863533
J Bioenerg Biomembr. 1986 Oct;18(5):331-68
3021714
Biochemistry. 1985 Oct 22;24(22):6298-304
3002436
Science. 2000 Mar 3;287(5458):1615-22
10698731
Biochem J. 1997 Aug 15;326 ( Pt 1):109-15
9337857
J Biol Inorg Chem. 2008 Jun;13(5):713-21
18317818
Eur J Biochem. 2001 Aug;268(15):4207-16
11488914
J Biol Chem. 2001 Jun 29;276(26):23357-61
11283020
J Biol Chem. 1995 May 26;270(21):12709-16
7759524
Adv Enzymol Relat Subj Biochem. 1956;17:65-134
13313307
Eur J Biochem. 1995 Jan 15;227(1-2):588-95
7851442
Biochem J. 1952 Feb;50(4):440-8
14925115
Biochemistry. 2004 Feb 3;43(4):1003-9
14744145
J Biol Chem. 1972 Nov 10;247(21):6932-6
4343163
Int J Biochem Cell Biol. 1998 Apr;30(4):457-63
9675879
J Biol Chem. 1992 May 25;267(15):10266-73
1316895
J Biol Chem. 1997 Feb 7;272(6):3520-6
9013599
FEBS Lett. 1998 Jan 23;422(1):1-4
9475157
Biochem J. 1982 May 1;203(2):483-92
6288002
Mol Cell. 2002 Feb;9(2):423-32
11864614
FEBS Lett. 1988 Oct 10;238(2):295-9
2844603
Biochemistry. 1964 Aug;3:1045-9
14220663
Biophys Chem. 2009 Sep;144(1-2):21-6
19545938
Biochemistry. 1993 Oct 12;32(40):10912-22
8399241
J Biol Chem. 2003 Jan 24;278(4):2356-60
12435729
Methods Enzymol. 1999;300:58-62
9919509
Biochem J. 1959 Mar;71(3):570-2
13638266
Biochim Biophys Acta. 1998 May 8;1380(3):336-44
9555085
Arch Biochem Biophys. 1995 Dec 1;324(1):135-42
7503548
Biochemistry. 1990 Feb 13;29(6):1661-8
2159343
Biochemistry. 1969 Jan;8(1):149-53
5777317
Biochemistry. 1999 Nov 9;38(45):14966-72
10555978
Biochem J. 1989 Jun 1;260(2):359-64
2764876
Anal Biochem. 2006 Dec 15;359(2):262-4
17049476
Free Radic Biol Med. 2007 Nov 15;43(10):1423-38
17936188
FEBS Lett. 2007 Feb 6;581(3):437-42
17239857