Comprehensive embryo analysis of advanced maternal age-related aneuploidies and mosaicism by short comparative genomic hybridization.
The short comparative genomic hybridization (short-CGH) method was used to perform a comprehensive cytogenetic study of isolated blastomeres from advanced maternal age embryos, discarded after fluorescent in situ hybridization (FISH) preimplantation genetic screening (PGS), detecting aneuploidies (38.5% of which corresponded to chromosomes not screened by 9-chromosome FISH), structural aberrations (31.8%), and mosaicism (77.3%). The short-CGH method was subsequently applied in one PGS, achieving a twin pregnancy.
Rius
Mariona
M
Unitat de Biologia Cellular i Genètica Mèdica, Facultat de Medicina, Universitat Autònoma de Barcelona, Bellaterra, Spain. mariona.rius@uab.cat
Daina
Gemma
G
Obradors
Albert
A
Ramos
Laia
L
Velilla
Esther
E
Fernández
Sílvia
S
Martínez-Passarell
Olga
O
Benet
Jordi
J
Navarro
Joaquima
J
D000026:Abortion, Habitual / Q000175:diagnosis; D000328:Adult; D000782:Aneuploidy; D001755:Blastocyst / Q000502:physiology; D002869:Chromosome Aberrations; D055028:Comparative Genomic Hybridization / Q000379:methods*; D005260:Female; D005820:Genetic Testing / Q000379:methods; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008423:Maternal Age; D009030:Mosaicism; D011247:Pregnancy; D011256:Pregnancy Outcome; D019836:Preimplantation Diagnosis / Q000379:methods*