Production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by Cupriavidus necator from waste rapeseed oil using propanol as a precursor of 3-hydroxyvalerate.
Waste rapeseed oil is a useful substrate for polyhydroxyalkanoates (PHA) production employing Cupriavidus necator H16. In fed-batch mode, we obtained biomass and PHA yields of 138 and 105 g l(-1), respectively. Yield coefficient and volumetric productivity were 0.83 g PHA per g oil and 1.46 g l(-1) h(-1), respectively. Propanol at 1% (v/v) enhanced both PHA and biomass formation significantly and, furthermore, resulted in incorporation of 3-hydroxyvalerate units into PHA structure. Thus, propanol can be used as an effective precursor of 3-hydroxyvalarete for production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolymer. During the fed-batch cultivation, propanol concentration was maintained at 1% which resulted in 8% content of 3-hydroxyvalerate in copolymer.
Obruca
Stanislav
S
Faculty of Chemistry, Department of Food Chemistry and Biotechnology, Brno University of Technology, Purkynova 118, 612 00, Brno, Czech Republic. Stana.O@seznam.cz
Marova
Ivana
I
Snajdar
Ondrej
O
Mravcova
Ludmila
L
Svoboda
Zdenek
Z
D000433:1-Propanol / Q000378:metabolism*; D018533:Biomass; D020118:Cupriavidus necator / Q000254:growth & development / Q000378:metabolism*; D005229:Fatty Acids, Monounsaturated; D007220:Industrial Waste; D010421:Pentanoic Acids / Q000378:metabolism*; D010938:Plant Oils / Q000378:metabolism*; D011091:Polyesters / Q000378:metabolism*; D000074262:Rapeseed Oil; D000094122:Polyhydroxybutyrates
D000433:1-Propanol; D005229:Fatty Acids, Monounsaturated; D007220:Industrial Waste; D010421:Pentanoic Acids; D010938:Plant Oils; D011091:Polyesters; D000074262:Rapeseed Oil; C052620:poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate); D000094122:Polyhydroxybutyrates; C013056:beta-hydroxyvaleric acid