title: Effective formation of the segregation-competent complex determines successful partitioning of the bovine papillomavirus genome during cell division.
abstract: Effective segregation of the bovine papillomavirus type 1 (BPV1), Epstein-Barr virus (EBV), and Kaposi's sarcoma-associated human herpesvirus type 8 (KSHV) genomes into daughter cells is mediated by a single viral protein that tethers viral genomes to host mitotic chromosomes. The linker proteins that mediate BPV1, EBV, and KSHV segregation are E2, LANA1, and EBNA1, respectively. The N-terminal transactivation domain of BPV1 E2 is responsible for chromatin attachment and subsequent viral genome segregation. Because E2 transcriptional activation and chromatin attachment functions are not mutually exclusive, we aimed to determine the requirement of these activities during segregation by analyzing chimeric E2 proteins. This approach allowed us to separate the two activities. Our data showed that attachment of the segregation protein to chromatin is not sufficient for proper segregation. Rather, formation of a segregation-competent complex which carries multiple copies of the segregation protein is required. Complementation studies of E2 functional domains indicated that chromatin attachment and transactivation functions must act in concert to ensure proper plasmid segregation. These data indicate that there are specific interactions between linker molecules and transcription factors/complexes that greatly increase segregation-competent complex formation. We also showed, using hybrid E2 molecules, that restored segregation function does not involve interactions with Brd4.
authors.affiliation: Department of Biomedical Technology, Institute of Technology, University of Tartu, Nooruse 1, 50411 Tartu, Estonia.
authors.forename: Toomas
authors.initials: T
authors.lastname: Silla
authors.forename: Andres
authors.initials: A
authors.lastname: Männik
authors.forename: Mart
authors.initials: M
authors.lastname: Ustav
mesh_terms: D000818:Animals; D052688:Bovine papillomavirus 1 / Q000235:genetics*; D002417:Cattle; D018797:Cell Cycle Proteins; D002455:Cell Division; D002843:Chromatin / Q000378:metabolism; D020090:Chromosome Segregation; D004268:DNA-Binding Proteins / Q000235:genetics; D016679:Genome, Viral; D019288:Herpesvirus 8, Human / Q000235:genetics; D054884:Host-Pathogen Interactions; D006801:Humans; D008938:Mitosis; D009687:Nuclear Proteins; D014157:Transcription Factors; D014764:Viral Proteins / Q000235:genetics; D000092468:Bromodomain Containing Proteins
chemical_list: D018797:Cell Cycle Proteins; D002843:Chromatin; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D014157:Transcription Factors; D014764:Viral Proteins; C441289:BRD4 protein, human; D000092468:Bromodomain Containing Proteins; C051550:E2 protein, Bovine papillomavirus
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