title: Toll-like Receptor3-mediated Induction of Chemokines in Salivary Epithelial Cells.
abstract: Toll-like receptors (TLRs) functionally expressed in salivary epithelial cells, but their roles remain elusive. Among TLRs family, TLR3 is activated by dsRNA, a byproduct of viral infection. The aim of this study was to investigate the role of TLR3 in the inflammatory immune responses using HSG cells. Reverse transcriptase-polymerase chain reaction (RT-PCR), real-time PCR and ELISA were performed to identify expression of TLRs and TLR3-mediated chemokine inductions. The chemotaxis assay of activated T lymphocytes was also performed. Treatment of HSG cells with polyinosinic: polycytidylic acid (poly(I:C)) significantly increased interferon-γ-inducible protein 10 (IP-10), interferoninducible T-cell α chemoattractant (I-TAC), and regulated on activation, normal T-cells expressed and secreted (RANTES) gene expressions in a concentration-dependent manner. Anti-TLR3 antibody blocked the increases of IP-10 and I-TAC genes. Poly(I:C)-induced increases of IP-10 and I-TAC were also confirmed at protein levels from cell lysates, but their release into extracellular medium was detected only in IP-10. We found that the culture media from HSG cells stimulated with poly(I:C) significantly increases T lymphocyte migration. Our results suggest that TLR3 plays an important role in chemokine induction, particularly IP-10, in salivary epithelial cells.
authors.affiliation: Department of Physiology, School of Dentistry, Seoul National University and Dental Research Institute, Seoul 110-749, Korea.
authors.forename: Jingchao
authors.initials: J
authors.lastname: Li
authors.forename: Mi Young
authors.initials: MY
authors.lastname: Jeong
authors.forename: Ji Hyun
authors.initials: JH
authors.lastname: Bae
authors.forename: Yong Hwan
authors.initials: YH
authors.lastname: Shin
authors.forename: Meihong
authors.initials: M
authors.lastname: Jin
authors.forename: Sung Min
authors.initials: SM
authors.lastname: Hang
authors.forename: Jeong Chai
authors.initials: JC
authors.lastname: Lee
authors.forename: Sung Joong
authors.initials: SJ
authors.lastname: Lee
authors.forename: Kyungpyo
authors.initials: K
authors.lastname: Park
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