title: Clinical microfluidics for neutrophil genomics and proteomics.
abstract: Neutrophils have key roles in modulating the immune response. We present a robust methodology for rapidly isolating neutrophils directly from whole blood with 'on-chip' processing for mRNA and protein isolation for genomics and proteomics. We validate this device with an ex vivo stimulation experiment and by comparison with standard bulk isolation methodologies. Last, we implement this tool as part of a near-patient blood processing system within a multi-center clinical study of the immune response to severe trauma and burn injury. The preliminary results from a small cohort of subjects in our study and healthy controls show a unique time-dependent gene expression pattern clearly demonstrating the ability of this tool to discriminate temporal transcriptional events of neutrophils within a clinical setting.
authors.affiliation: Department of Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA. kkotz@partners.org
authors.forename: Kenneth T
authors.initials: KT
authors.lastname: Kotz
authors.forename: Wenzong
authors.initials: W
authors.lastname: Xiao
authors.forename: Carol
authors.initials: C
authors.lastname: Miller-Graziano
authors.forename: Wei-Jun
authors.initials: WJ
authors.lastname: Qian
authors.forename: Aman
authors.initials: A
authors.lastname: Russom
authors.forename: Elizabeth A
authors.initials: EA
authors.lastname: Warner
authors.forename: Lyle L
authors.initials: LL
authors.lastname: Moldawer
authors.forename: Asit
authors.initials: A
authors.lastname: De
authors.forename: Paul E
authors.initials: PE
authors.lastname: Bankey
authors.forename: Brianne O
authors.initials: BO
authors.lastname: Petritis
authors.forename: David G
authors.initials: DG
authors.lastname: Camp
authors.forename: Alan E
authors.initials: AE
authors.lastname: Rosenbach
authors.forename: Jeremy
authors.initials: J
authors.lastname: Goverman
authors.forename: Shawn P
authors.initials: SP
authors.lastname: Fagan
authors.forename: Bernard H
authors.initials: BH
authors.lastname: Brownstein
authors.forename: Daniel
authors.initials: D
authors.lastname: Irimia
authors.forename: Weihong
authors.initials: W
authors.lastname: Xu
authors.forename: Julie
authors.initials: J
authors.lastname: Wilhelmy
authors.forename: Michael N
authors.initials: MN
authors.lastname: Mindrinos
authors.forename: Richard D
authors.initials: RD
authors.lastname: Smith
authors.forename: Ronald W
authors.initials: RW
authors.lastname: Davis
authors.forename: Ronald G
authors.initials: RG
authors.lastname: Tompkins
authors.forename: Mehmet
authors.initials: M
authors.lastname: Toner
mesh_terms: D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000235:genetics / Q000276:immunology; D019921:Biotinylation; D002056:Burns / Q000503:physiopathology*; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000276:immunology; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D058851:GPI-Linked Proteins; D023281:Genomics / Q000379:methods*; D006801:Humans; D044085:Microfluidics / Q000379:methods*; D009504:Neutrophils / Q000166:cytology / Q000502:physiology*; D020411:Oligonucleotide Array Sequence Analysis; D040901:Proteomics / Q000379:methods*; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D014947:Wounds and Injuries / Q000503:physiopathology
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; C504120:CEACAM8 protein, human; D015815:Cell Adhesion Molecules; D058851:GPI-Linked Proteins; D012313:RNA; D004247:DNA
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