title: Region-specific changes in ascorbate concentration during rat brain development quantified by in vivo (1)H NMR spectroscopy.
abstract: Ascorbate (vitamin C, Asc) was quantified in vivo using short-TE (1)H NMR spectra from a previously published study on regional and developmental changes in the neurochemical profile of the rat brain (Tkac I, Rao R, Georgieff MK, Gruetter R. Magn Reson Med. 2003; 50: 24-32). Asc concentration was quantified on postnatal days P7-P28 from three regions that are of interest in the study of neurocognitive development, i.e. the hippocampus, striatum and cerebral cortex. The previously measured (1)H NMR spectra were re-analyzed using LCModel with the Asc spectrum included in the basis set. The Asc concentration was consistently quantified from all 110 re-analyzed spectra with an estimated fitting error of 7% (i.e. the average Cramer-Rao lower bound). The sensitivity of Asc quantification was sufficiently high to detect regional and developmental changes in Asc concentration. The concentration of Asc was highest on P7, and decreased with age in all three brain regions (p < 0.001) in agreement with previous in vitro studies. At P10 and older postnatal ages, an inhomogeneous distribution of Asc among brain regions was detected. In addition to facilitating the quantification of this important antioxidant concentration, the inclusion of the Asc spectrum in the LCModel basis set improved the quantification accuracy of other brain metabolite concentrations in the neurochemical profile.
authors.affiliation: Radiology and Center for Magnetic Resonance Research, University of Minnesota, Minneapolis, MN, USA. melissa@cmrr.umn.edu
authors.forename: Melissa
authors.initials: M
authors.lastname: Terpstra
authors.forename: Raghavendra
authors.initials: R
authors.lastname: Rao
authors.forename: Ivan
authors.initials: I
authors.lastname: Tkac
mesh_terms: D000375:Aging / Q000502:physiology; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D001205:Ascorbic Acid / Q000378:metabolism*; D001921:Brain / Q000033:anatomy & histology / Q000254:growth & development* / Q000378:metabolism*; D001923:Brain Chemistry; D006801:Humans; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D051381:Rats
chemical_list: D000975:Antioxidants; D001205:Ascorbic Acid
references.citation: Magn Reson Med. 2004 Feb;51(2):225-9
references.pmid: 14755644
references.citation: J Neurochem. 2007 Jun;101(6):1505-15
references.pmid: 17437538
references.citation: Magn Reson Med. 2004 Sep;52(3):478-84
references.pmid: 15334565
references.citation: NMR Biomed. 2001 Jun;14(4):260-4
references.pmid: 11410943
references.citation: Indian J Biochem. 1971 Dec;8(4):295-9
references.pmid: 4268990
references.citation: J Neurochem. 2007 Mar;100(5):1397-406
references.pmid: 17217418
references.citation: Brain Res. 2008 Aug 11;1224:119-26
references.pmid: 18582442
references.citation: Comp Biochem Physiol C Toxicol Pharmacol. 2002 Dec;133(4):515-25
references.pmid: 12458180
references.citation: Pediatr Res. 2009 Dec;66(6):642-7
references.pmid: 19687776
references.citation: J Cereb Blood Flow Metab. 2001 Jun;21(6):653-63
references.pmid: 11488534
references.citation: Magn Reson Med. 2006 May;55(5):979-83
references.pmid: 16586452
references.citation: Magn Reson Med. 2008 Sep;60(3):727-31
references.pmid: 18727088
references.citation: J Cereb Blood Flow Metab. 2009 Apr;29(4):811-9
references.pmid: 19223915
references.citation: Neuroscience. 1998 Feb;82(4):1213-23
references.pmid: 9466441
references.citation: Magn Reson Med. 1993 Dec;30(6):672-9
references.pmid: 8139448
references.citation: NMR Biomed. 2010 Apr;23(3):227-32
references.pmid: 19655342
references.citation: Magn Reson Med. 2003 Jul;50(1):24-32
references.pmid: 12815675
references.citation: Nat Med. 2002 May;8(5):445-6
references.pmid: 11984580
references.citation: Environ Health Perspect. 2000 Jun;108 Suppl 3:511-33
references.pmid: 10852851
references.citation: J Magn Reson. 1999 Nov;141(1):104-20
references.pmid: 10527748
references.citation: Neurosci Biobehav Rev. 1997 Mar;21(2):167-74
references.pmid: 9062939
references.citation: J Neurochem. 1967 Feb;14(2):161-7
references.pmid: 6020705