title: A synthetic biology approach allows inducible retrotransposition in whole plants.
abstract: Retrotransposons are mobile genetic elements that transpose by reverse transcription of element RNA, followed by insertion of the cDNA into new positions of the host genome. Although they are major constituents of eukaryotic genomes, many facets of their biology remain to be understood. Transposition is generally rare, suggesting that it is subject to tight regulation. However, only the first regulatory step (transcriptional induction) is currently amenable to investigation in higher eukaryotes. To investigate the complete life cycle of a long terminal repeat (LTR) retrotransposon in plants, we established a synthetic biology program on tobacco retrotransposon Tto1, and achieved transposition in whole plants triggered by an inducible promoter. The engineered element, iTto (inducible Tto1), is a novel tool for analysis of retrotransposition in plants. In addition, it allows to explore the potential of an inducible retrotransposon for insertional mutagenesis.
  
  
  BACKGROUND
  The online version of this article (doi:10.1007/s11693-010-9053-4) contains supplementary material, which is available to authorized users.
authors.forename: Gudrun
authors.initials: G
authors.lastname: Böhmdorfer
authors.forename: Andrea
authors.initials: A
authors.lastname: Tramontano
authors.forename: Kerstin
authors.initials: K
authors.lastname: Luxa
authors.forename: Andreas
authors.initials: A
authors.lastname: Bachmair
references.citation: Nature. 2009 Sep 17;461(7262):423-6
references.pmid: 19734880
references.citation: Plant J. 1998 Dec;16(6):735-43
references.pmid: 10069079
references.citation: Plant J. 2000 Jul;23(2):291-304
references.pmid: 10929123
references.citation: Nat Rev Genet. 2005 Feb;6(2):128-36
references.pmid: 15640810
references.citation: Mol Plant Microbe Interact. 2003 Dec;16(12):1069-76
references.pmid: 14651340
references.citation: Plant Mol Biol. 1998 Feb;36(3):365-76
references.pmid: 9484477
references.citation: Nat Rev Genet. 2002 May;3(5):329-41
references.pmid: 11988759
references.citation: Nature. 2009 Sep 17;461(7262):427-30
references.pmid: 19734882
references.citation: Annu Rev Genet. 2002;36:489-519
references.pmid: 12429701
references.citation: Plant J. 1999 Oct;20(1):127-33
references.pmid: 10571872
references.citation: Curr Opin Genet Dev. 2005 Dec;15(6):621-7
references.pmid: 16219458
references.citation: Planta. 2001 Jul;213(3):370-8
references.pmid: 11506359
references.citation: Plant J. 1997 Mar;11(3):605-12
references.pmid: 9107046
references.citation: Nature. 2001 May 10;411(6834):212-4
references.pmid: 11346800
references.citation: Nat Rev Genet. 2005 Jan;6(1):24-35
references.pmid: 15630419
references.citation: Plant Cell. 2007 Jan;19(1):9-22
references.pmid: 17259261
references.citation: RNA. 2005 Aug;11(8):1181-91
references.pmid: 16043504
references.citation: EMBO J. 1993 Jun;12(6):2521-8
references.pmid: 8389699
references.citation: Nat Genet. 1999 Feb;21(2):209-12
references.pmid: 9988275
references.citation: Plant J. 2000 Oct;24(2):265-73
references.pmid: 11069700
references.citation: Virology. 2008 Apr 10;373(2):437-46
references.pmid: 18191436
references.citation: Plant Physiol. 2008 Jul;147(3):1264-78
references.pmid: 18467467
references.citation: Plant Cell. 1996 Apr;8(4):725-34
references.pmid: 8624443
references.citation: Plant Mol Biol. 1997 Jan;33(2):257-66
references.pmid: 9037144
references.citation: Mol Genet Genomics. 2006 Oct;276(4):378-90
references.pmid: 16821043