Bartonella genotypes in fleas (insecta: siphonaptera) collected from rodents in the negev desert, Israel.
Fleas collected from rodents in the Negev Desert in southern Israel were molecularly screened for Bartonella species. A total of 1,148 fleas, collected from 122 rodents belonging to six species, were pooled in 245 pools based on flea species, sex, and rodent host species. Two Bartonella gene fragments, corresponding to RNA polymerase B (rpoB) and citrate synthase (gltA), were targeted, and 94 and 74 flea pools were found positive by PCR, respectively. The Bartonella 16S-23S internal transcribed spacer (ITS) region was also targeted, and 66 flea pools were found to be positive by PCR. Sixteen different Bartonella gltA genotypes were detected in 94 positive flea pools collected from 5 different rodent species, indicating that fleas collected from each rodent species can harbor several Bartonella genotypes. Based on gltA analysis, identified Bartonella genotypes were highly similar or identical to strains previously detected in rodent species from different parts of the world. A gltA fragment 100% similar to Bartonella henselae was detected in one flea pool. Another 2 flea pools contained gltA fragments that were closely related to B. henselae (98% similarity). The high sequence similarities to the zoonotic pathogen B. henselae warrant further investigation.
Morick
Danny
D
Koret School of Veterinary Medicine, The Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel.
Krasnov
Boris R
BR
Khokhlova
Irina S
IS
Shenbrot
Georgy I
GI
Kosoy
Michael Y
MY
Harrus
Shimon
S
D000818:Animals; D001473:Bartonella / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D002950:Citrate (si)-Synthase / Q000235:genetics; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D005838:Genotype; D007557:Israel; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012377:Rodentia / Q000469:parasitology*; D017422:Sequence Analysis, DNA; D005423:Siphonaptera / Q000382:microbiology*
D004269:DNA, Bacterial; D021903:DNA, Ribosomal Spacer; D002950:Citrate (si)-Synthase; D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit
Med Vet Entomol. 2008 Mar;22(1):1-15
18380649
Vector Borne Zoonotic Dis. 2008 Aug;8(4):467-74
18399782
N Engl J Med. 1990 Dec 6;323(23):1573-80
2233945
Parasitology. 1997 Feb;114 ( Pt 2):159-73
9051922
J Clin Microbiol. 1999 Aug;37(8):2598-601
10405408
Vet Microbiol. 2009 Nov 18;139(3-4):293-7
19595521
Parasitology. 2008 Aug;135(9):1133-41
18561869
J Clin Microbiol. 2003 Feb;41(2):645-50
12574261
J Infect Dis. 1999 Jul;180(1):220-4
10353885
J Clin Microbiol. 2005 Mar;43(3):1171-6
15750079
J Med Microbiol. 2008 Dec;57(Pt 12):1496-1501
19018019
Epidemiol Infect. 2001 Apr;126(2):323-9
11349984
Appl Environ Microbiol. 2007 Oct;73(19):6045-52
17693558
Int J Syst Evol Microbiol. 2004 Nov;54(Pt 6):1959-1967
15545418
Vet Microbiol. 2010 Jan 27;140(3-4):347-59
20018462
APMIS. 2004 Jun;112(6):336-41
15511270
Am J Trop Med Hyg. 2009 Jul;81(1):55-8
19556567
Comp Immunol Microbiol Infect Dis. 2000 Oct;23(4):221-38
11038125
Am J Trop Med Hyg. 1997 Nov;57(5):578-88
9392599
ISME J. 2010 Jul;4(7):955-8
20220787
EXS. 2000;88:215-31
10803381
Vector Borne Zoonotic Dis. 2006 Winter;6(4):395-403
17187575
J Clin Microbiol. 2009 Jul;47(7):2332-5
19439538
Emerg Infect Dis. 2009 Apr;15(4):526-32
19331727
J Clin Microbiol. 1999 Dec;37(12):4034-8
10565926
J Clin Microbiol. 1995 Jul;33(7):1797-803
7545181
Nucleic Acids Res. 1994 Nov 11;22(22):4673-80
7984417