Aptamer-based biosensor for sensitive PDGF detection using diamond transistor.
The detection of platelet-derived growth factor (PDGF) via a solution-gate field-effect transistor (SGFET) has been demonstrated for the first time using aptamers immobilized on a diamond surface. Upon introduction of PDGF to the immobilized aptamer, a shift of 31.7 mV in the negative direction is observed at a source-drain current of -50 μA. A shift of 32.3 mV in the positive direction is detected after regeneration by SDS solution, indicating that the static measurement returns to its original value. These SGFETs operate stably within the large potential window of diamond (>3.0 V), and hence the surface channel does not need passivating with a thick insulating layer. Thereof, the immobilized aptamer channels have been exposed directly to the electrolyte solution without a gate insulator. Immobilization is achieved via aptamers covalently bonding to amine sites, thereby increasing the sensitivity of the biosensors. Diamond SGFETs have potential for the detection of PDGF and show durability against biological degradation after repeated usage and regeneration.
Ruslinda
A Rahim
AR
School of Science and Engineering, Waseda University, 3-4-1 Okubo, Shinjuku-ku, Tokyo 169-8555, Japan. ruslinda@toki.waseda.jp
Tajima
Shinya
S
Ishii
Yoko
Y
Ishiyama
Yuichiro
Y
Edgington
Robert
R
Kawarada
Hiroshi
H
D052157:Aptamers, Nucleotide / Q000235:genetics; D001483:Base Sequence; D000077214:Becaplermin; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000706:statistics & numerical data; D018130:Diamond; D017743:Equipment Reuse; D010982:Platelet-Derived Growth Factor / Q000032:analysis*; D020574:Proto-Oncogene Proteins c-sis; D012680:Sensitivity and Specificity; D014173:Transistors, Electronic
D052157:Aptamers, Nucleotide; D010982:Platelet-Derived Growth Factor; D020574:Proto-Oncogene Proteins c-sis; C075278:platelet-derived growth factor A; C095477:platelet-derived growth factor AB; D000077214:Becaplermin; D018130:Diamond