5-S-GAD attenuates Fe²+-induced lipid peroxidation and cell death in a neuronal cell model.
Iron accumulation in brain is associated with a number of common neurodegenerative disorders. N-β-alanyl-5-S-glutathionyl-3,4-dihydroxyphenylalanine (5-S-GAD), a novel catechol derivative, was isolated from adult flesh fly as a defense substance. We examined the effect of 5-S-GAD on Fe²+-induced lipid peroxidation and cell death in PC12 cells. Treatment of PC12 cells with Fe²+ (1-20 μM) for 24 h induced lipid peroxidation and cell death in a dose-dependent manner. Butylated hydroxyanisole and α-tocopherol inhibited Fe²+-induced lipid peroxidation and cell death. 5-S-GAD inhibited Fe²+-induced lipid peroxidation and cell death. 5-S-GAD protected PC12 cells from Fe²+-induced cell death possibly by blocking lipid peroxidation.
Takadera
Tsuneo
T
Center of Development for Education, Hokuriku University, Kanazawa, 920-1181, Japan. t-takadera@hokuriku-u.ac.jp
Koriyama
Yoshiki
Y
Kimura
Takahito
T
Kato
Satoru
S
D000818:Animals; D002083:Butylated Hydroxyanisole / Q000494:pharmacology; D016923:Cell Death / Q000187:drug effects* / Q000502:physiology; D004295:Dihydroxyphenylalanine / Q000031:analogs & derivatives* / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005296:Ferrous Compounds / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D005978:Glutathione / Q000031:analogs & derivatives* / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects*; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D016716:PC12 Cells; D051381:Rats; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D024505:Tocopherols / Q000494:pharmacology
D005296:Ferrous Compounds; C100496:N-beta-alanyl-5-S-glutathionyl-3,4-dihydroxyphenylalanine; D017392:Thiobarbituric Acid Reactive Substances; D002083:Butylated Hydroxyanisole; C020748:ferrous sulfate; D004295:Dihydroxyphenylalanine; D005978:Glutathione; D024505:Tocopherols
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