title: Investigation of platelet responses and clotting characteristics of in situ albumin binding surfaces.
abstract: The response of biomaterial surfaces when exposed to blood is in part dependent upon the nature and composition of the adsorbed layer of proteins. Surfaces passivated with albumin have been shown to reduce platelet adhesion and activation. In an attempt to develop surfaces that can selectively and specifically bind albumin, silicon-based surfaces were functionalized with linear peptides and chemical ligands that displayed an affinity for albumin. Peptide functionalized surfaces were observed to preferentially bind albumin when compared to human immunoglobulin and human fibrinogen, which possess low densities of surface adsorbed platelets. The platelet morphology was noted to be discoid on the peptide modified surface. Both the unmodified control and SCL functionalized surfaces had high densities of surface adhered platelets with spread out morphology. The peptide and SCL functionalized surfaces were noted to have no impact on PTT and PT clotting times, indicating that the extrinsic and intrinsic pathways were unperturbed by the surfaces generated.
authors.affiliation: Chemical and Biomolecular Engineering, University of Nebraska Othmer Hall, Lincoln, NE 68588, USA.
authors.forename: Sanjukta
authors.initials: S
authors.lastname: Guha Thakurta
authors.forename: Robert
authors.initials: R
authors.lastname: Miller
authors.forename: Anuradha
authors.initials: A
authors.lastname: Subramanian
mesh_terms: D000595:Amino Acid Sequence; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism*; D001780:Blood Coagulation Tests; D001792:Blood Platelets / Q000166:cytology* / Q000201:enzymology; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000378:metabolism*; D010973:Platelet Adhesiveness; D011485:Protein Binding; D012709:Serum Albumin / Q000378:metabolism*; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D010455:Peptides; D012709:Serum Albumin; D007770:L-Lactate Dehydrogenase