Sequence analysis of chromatin immunoprecipitation data for transcription factors.
Chromatin immunoprecipitation (ChIP) experiments allow the location of transcription factors to be determined across the genome. Subsequent analysis of the sequences of the identified regions allows binding to be localized at a higher resolution than can be achieved by current high-throughput experiments without sequence analysis and may provide important insight into the regulatory programs enacted by the protein of interest. In this chapter we review the tools, workflow, and common pitfalls of such analyses and recommend strategies for effective motif discovery from these data.
Macisaac
Kenzie D
KD
Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA. kenzie_macisaac@merck.edu
Fraenkel
Ernest
E
D001665:Binding Sites; D047369:Chromatin Immunoprecipitation / Q000379:methods*; D002874:Chromosome Mapping; D004247:DNA / Q000235:genetics / Q000378:metabolism; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000379:methods*; D013379:Substrate Specificity; D014157:Transcription Factors / Q000378:metabolism*
D014157:Transcription Factors; D004247:DNA
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