Contrast-enhanced digital holographic imaging of cellular structures by manipulating the intracellular refractive index.
The understanding of biological reactions and evaluation of the significance for living cells strongly depends on the ability to visualize and quantify these processes. Digital holographic microscopy (DHM) enables quantitative phase contrast imaging for high resolution and minimal invasive live cell analysis without the need of labeling or complex sample preparation. However, due to the rather homogeneous intracellular refractive index, the phase contrast of subcellular structures is limited and often low. We analyze the impact of the specific manipulation of the intracellular refractive index by microinjection on the DHM phase contrast. Glycerol is chosen as osmolyte, which combines high solubility in aqueous solutions and biological compatibility. We show that the intracellular injection of glycerol causes a contrast enhancement that can be explained by a decrease of the cytosolic refractive index due to a water influx. The underlying principle is proven by experiments inducing cell shrinkage and with fixated cells. The integrity of the cell membrane is considered as a prerequisite and allows a reversible cell swelling and shrinking within a certain limit. The presented approach to control the intracellular phase contrast demonstrated for the example of DHM opens prospects for applications with other quantitative phase contrast imaging methods.
Rommel
Christina E
CE
University of Muenster, Department of Medicine B, Gastroenterological Molecular Cell Biology, Muenster, Germany.
Dierker
Christian
C
Schmidt
Lisa
L
Przibilla
Sabine
S
von Bally
Gert
G
Kemper
Björn
B
Schnekenburger
Jürgen
J
D000818:Animals; D003287:Contrast Media; D006696:Holography / Q000379:methods*; D006801:Humans; D007089:Image Enhancement / Q000379:methods*; D008846:Micromanipulation / Q000379:methods*; D012031:Refractometry / Q000379:methods*; D012815:Signal Processing, Computer-Assisted; D013347:Subcellular Fractions / Q000648:ultrastructure*
D003287:Contrast Media