[Experimental model of canine oleic acid-induced acute lung injury: establishment of a working model].
BACKGROUND
There are several experimental model of acute lung injury induced by oleic acid (OA); however, there are few studies that show how this injury develops.


OBJECTIVE
This study seeks to detail the x-ray, hemodynamic, gasometrical, gravimetrical, macroscopic and microscopic alterations developed in an experimental model of canine OA-induced acute lung injury (ALI).


METHODS
Twelve dogs were divided in 2 study groups: Group I (n=6): Control group without ALI. Group II (n=6); OA-induced ALI. All dogs were submitted to X-ray, hemodynamic and gasometric evaluation before ALI induction, and later every 15 minutes during 150 minutes. At the end of the study, the animals were euthanatized and were evaluated the changes gravimetric, macroscopic and microscopic in injured lungs.


RESULTS
All the animals survived through the study. In group II, 100% of the animals developed x-ray (p < 0.003 Wilcoxon), hemodynamic, gasometrical and gravimetric (p < 0.5 ANOVA, Tukey), macroscopically and microscopically (p < 0.001 Wilcoxon) ALI.


CONCLUSIONS
The OA-induced ALI is a model in which dogs develop X-ray, hemodynamic, gasometrical, gravimetrical, macroscopically and microscopically injuries of the exudative phase that lung with ALI injury presents.
Hernández-Jiménez
Claudia
C
Departamento de Cirugía Experimental, Instituto Nacional de Enfermedades Respiratorias, Ismael Cosio-Villegas, DF México. claudia_herjim@yahoo.com
Jasso-Victoria
Rogelio
R
Olmos-Zúñiga
Raúl
R
Villalba-Caloca
Jaime
J
Gaxiola-Gaxiola
Miguel
M
Sotres-Vega
Avelina
A
Baltazares-Lipp
Matilde
M
Villa-Godoy
Alejandro
A
D055371:Acute Lung Injury / Q000139:chemically induced; D000818:Animals; D004195:Disease Models, Animal; D004285:Dogs; D019301:Oleic Acid / Q000008:administration & dosage
D019301:Oleic Acid