title: Reinforcement of colonic anastomoses with a collagenous double-layer matrix extracted from porcine dermis.
abstract: BACKGROUND
  Anastomotic leakage is a major factor for morbidity in colorectal surgery. Anastomotic reinforcement with biological or synthetic materials has been claimed to be useful in preventing anastomotic leakage.
  
  
  METHODS
  We evaluated a non-cross-linked collagenous matrix Bio-Gide (BG) for sealing colonic anastomoses in a rodent model. The animals were investigated for 4, 30 and 90 days. Macroscopic examination, histological examination and measurement of bursting pressure were performed. The anastomotic stricture rate was evaluated by radiographic contrast enema.
  
  
  RESULTS
  Microscopically anastomoses sealed by BG showed impaired anastomotic healing. Blood vessel ingrowth and collagen deposition were decreased without reaching significance after 4 days. The anastomotic bursting pressure was significantly decreased (p = 0.0454) in the early phase of healing. Anastomotic neovascularization was significantly decreased compared to the control group after 30 (p = 0.0058) and 90 days (p = 0.0275). Although no difference in anastomotic stricture rate was evident, the rate of intra-abdominal adhesions was significantly increased after 30 (p = 0.0124) and 90 days (p = 0.0281).
  
  
  CONCLUSIONS
  BG failed to improve colonic anastomotic healing. Early anastomotic healing was impaired if anastomoses were reinforced with BG. BG did not affect the anastomotic stricture rate for up to 3 months; nevertheless, intra-abdominal adhesions were increased.
authors.affiliation: Department of General and Digestive Surgery, University of Freiburg, Freiburg, Germany. jens.hoeppner @ uniklinik-freiburg.de
authors.forename: J
authors.initials: J
authors.lastname: Hoeppner
authors.forename: K
authors.initials: K
authors.lastname: Willa
authors.forename: S
authors.initials: S
authors.lastname: Timme
authors.forename: D
authors.initials: D
authors.lastname: Tittelbach-Helmrich
authors.forename: U T
authors.initials: UT
authors.lastname: Hopt
authors.forename: T
authors.initials: T
authors.lastname: Keck
authors.forename: G
authors.initials: G
authors.lastname: Marjanovic
mesh_terms: D000714:Anastomosis, Surgical / Q000009:adverse effects / Q000379:methods*; D057868:Anastomotic Leak / Q000209:etiology / Q000517:prevention & control; D000818:Animals; D003094:Collagen / Q000009:adverse effects; D003106:Colon / Q000098:blood supply / Q000473:pathology / Q000601:surgery*; D020405:Dermis / Q000737:chemistry; D008297:Male; D008422:Materials Testing; D008567:Membranes, Artificial; D018919:Neovascularization, Physiologic; D051381:Rats; D017208:Rats, Wistar; D013552:Swine; D000267:Tissue Adhesions / Q000209:etiology / Q000517:prevention & control; D014945:Wound Healing
chemical_list: C106416:Bio-Gide; D008567:Membranes, Artificial; D003094:Collagen