title: IL-10 produced by activated human B cells regulates CD4(+) T-cell activation in vitro.
abstract: IL-10-producing regulatory B cells have been identified in mice and shown to downregulate inflammation, making them potentially important for maintenance of tolerance. In this study, we isolated B cells from human blood and spleen, and showed that after a short period of ex vivo stimulation a number of these cells produced IL-10. The IL-10-producing B cells did not fall within a single clearly defined subpopulation, but were enriched in both the memory (CD27(+)) and the transitional (CD38(high)) B-cell compartments. Combined CpG-B+anti-Ig stimulation was the most potent IL-10 stimulus tested. B cells stimulated in this way inhibited CD4(+)CD25(-) T-cell proliferation in vitro by a partially IL-10-dependent mechanism. These findings imply that manipulating IL-10 production by human B cells could be a useful therapeutic strategy for modulating immune responses in humans.
authors.affiliation: INSERM U976, Saint Louis Hospital, Skin Research Center, Paris, France. jean-david.bouaziz@sls.aphp.fr
authors.forename: Jean-David
authors.initials: JD
authors.lastname: Bouaziz
authors.forename: Sebastien
authors.initials: S
authors.lastname: Calbo
authors.forename: Maud
authors.initials: M
authors.lastname: Maho-Vaillant
authors.forename: Anne
authors.initials: A
authors.lastname: Saussine
authors.forename: Martine
authors.initials: M
authors.lastname: Bagot
authors.forename: Armand
authors.initials: A
authors.lastname: Bensussan
authors.forename: Philippe
authors.initials: P
authors.lastname: Musette
mesh_terms: D015551:Autoimmunity / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D049109:Cell Proliferation; D005434:Flow Cytometry; D006801:Humans; D007108:Immune Tolerance / Q000276:immunology; D016753:Interleukin-10 / Q000096:biosynthesis* / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology; D011947:Receptors, Antigen, B-Cell / Q000276:immunology; D051217:Toll-Like Receptor 9 / Q000276:immunology
chemical_list: D011947:Receptors, Antigen, B-Cell; C495371:TLR9 protein, human; D051217:Toll-Like Receptor 9; D016753:Interleukin-10