title: Inflammatory dendritic cells--not basophils--are necessary and sufficient for induction of Th2 immunity to inhaled house dust mite allergen.
abstract: It is unclear how Th2 immunity is induced in response to allergens like house dust mite (HDM). Here, we show that HDM inhalation leads to the TLR4/MyD88-dependent recruitment of IL-4 competent basophils and eosinophils, and of inflammatory DCs to the draining mediastinal nodes. Depletion of basophils only partially reduced Th2 immunity, and depletion of eosinophils had no effect on the Th2 response. Basophils did not take up inhaled antigen, present it to T cells, or express antigen presentation machinery, whereas a population of FceRI(+) DCs readily did. Inflammatory DCs were necessary and sufficient for induction of Th2 immunity and features of asthma, whereas basophils were not required. We favor a model whereby DCs initiate and basophils amplify Th2 immunity to HDM allergen.
authors.affiliation: Laboratory of Immunoregulation and Mucosal Immunology, Department of Respiratory Diseases, University of Ghent, 9000 Ghent, Belgium. hamida.hammad@ugent.be
authors.forename: Hamida
authors.initials: H
authors.lastname: Hammad
authors.forename: Maud
authors.initials: M
authors.lastname: Plantinga
authors.forename: Kim
authors.initials: K
authors.lastname: Deswarte
authors.forename: Philippe
authors.initials: P
authors.lastname: Pouliot
authors.forename: Monique A M
authors.initials: MA
authors.lastname: Willart
authors.forename: Mirjam
authors.initials: M
authors.lastname: Kool
authors.forename: Femke
authors.initials: F
authors.lastname: Muskens
authors.forename: Bart N
authors.initials: BN
authors.lastname: Lambrecht
mesh_terms: D000818:Animals; D017951:Antigen Presentation; D039741:Antigens, Dermatophagoides / Q000276:immunology*; D001249:Asthma / Q000209:etiology / Q000276:immunology; D001491:Basophils / Q000276:immunology*; D003713:Dendritic Cells / Q000276:immunology*; D004804:Eosinophils / Q000276:immunology; D007113:Immunity, Innate; D001239:Inhalation; D015847:Interleukin-4 / Q000276:immunology; D007958:Leukocyte Count; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D039981:Pyroglyphidae / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology*
chemical_list: D039741:Antigens, Dermatophagoides; D015847:Interleukin-4
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