title: Identification, tissue distribution and evaluation of brain neuropeptide Y gene expression in the Brazilian flounder Paralichthys orbignyanus.
abstract: Neuropeptide Y (NPY) is one of the most potent stimulants of food intake in vertebrates, mammals and fish. However, the present knowledge about feeding behaviour in fish is still limited and based on studies in a few species. The Brazilian flounder Paralichthys orbignyanus is being considered for aquaculture, and it is important to understand the mechanisms regulating feeding in order to improve its performance in captivity. The objectives of this study were to clone NPY cDNA, evaluate the mRNA levels in different tissues of flounder, and also evaluate brain NPY expression to associate food intake with NPY expression levels. A 597 bp NPY cDNA was cloned from Brazilian flounder brain. NPY expression was detected in all the peripheral tissues analysed. No significant differences were observed in brain NPY gene expression over 24 h after food intake at a temperature of 15 +or- 3 degrees C. No correlation was observed among plasma glucose, total protein, cholesterol, triglycerides and NPY expression levels during this 24 h period. On the other hand, mRNA levels were increased after two weeks of fasting at elevated temperatures. Our results suggest that NPY mRNA levels in Brazilian flounder are affected by temperature.
authors.affiliation: Centro de Desenvolvimento Tecnologico, Universidade Federal de Pelotas, CP 354, 96010-900, Pelotas, RS, Brazil. fariascampos@gmail.com
authors.forename: Vinicius F
authors.initials: VF
authors.lastname: Campos
authors.forename: Tiago
authors.initials: T
authors.lastname: Collares
authors.forename: João C
authors.initials: JC
authors.lastname: Deschamps
authors.forename: Fabiana K
authors.initials: FK
authors.lastname: Seixas
authors.forename: Odir A
authors.initials: OA
authors.lastname: Dellagostin
authors.forename: Carlos Frederico C
authors.initials: CF
authors.lastname: Lanes
authors.forename: Juliana
authors.initials: J
authors.lastname: Sandrini
authors.forename: Luis Fernando
authors.initials: LF
authors.lastname: Marins
authors.forename: Marcelo
authors.initials: M
authors.lastname: Okamoto
authors.forename: Luís A
authors.initials: LA
authors.lastname: Sampaio
authors.forename: Ricardo B
authors.initials: RB
authors.lastname: Robaldo
mesh_terms: D000818:Animals; D017756:Aquaculture; D001483:Base Sequence; D001921:Brain / Q000378:metabolism*; D005215:Fasting; D005247:Feeding Behavior / Q000502:physiology*; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism*; D005432:Flounder / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D008969:Molecular Sequence Data; D009478:Neuropeptide Y / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013696:Temperature
chemical_list: D029941:Fish Proteins; D009478:Neuropeptide Y; D012333:RNA, Messenger
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