Calcineurin regulates coelomocyte endocytosis via DYN-1 and CUP-4 in Caenorhabditis elegans.
C. elegans coelomocytes are macrophage-like scavenger cells that provide an excellent in vivo system for the study of clathrin-mediated endocytosis. Using this in vivo system, several genes involved in coelomocyte endocytosis have been identified previously. However, the detailed mechanism of endocytic pathway is still unknown. Here, we report a new function of calcineurin, an evolutionarily conserved Ca(2+)/calmodulin-dependent Ser/Thr protein phosphatase, in coelomocyte endocytosis. We found that calcineurin mutants show defective coelomocyte endocytosis. Genetic analysis suggests that calcineurin and a GTPase, dynamin (DYN-1), may function upstream of an orphan receptor, CUP-4, to regulate endocytosis. Therefore, we propose a model in which calcineurin may regulate coelomocyte endocytosis via DYN-1 and CUP-4 in C. elegans.
Song
Hyun-Ok
HO
Department of Life Science, College of Natural Sciences, Hanyang University, Seoul, 133-791, Korea.
Lee
Jungsoo
J
Ji
Yon Ju
YJ
Dwivedi
Meenakshi
M
Cho
Jeong Hoon
JH
Park
Byung-Jae
BJ
Ahnn
Joohong
J
D000818:Animals; D017173:Caenorhabditis elegans / Q000502:physiology*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism*; D019703:Calcineurin / Q000235:genetics / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D034281:Dynamins / Q000378:metabolism*; D004705:Endocytosis / Q000235:genetics; D009154:Mutation / Q000235:genetics; D015398:Signal Transduction
C504071:CUP-4 protein, C elegans; D029742:Caenorhabditis elegans Proteins; D019703:Calcineurin; C511792:Dyn-1 protein, C elegans; D034281:Dynamins; D002118:Calcium