Desulfovibrio legallis sp. nov.: a moderately halophilic, sulfate-reducing bacterium isolated from a wastewater digestor in Tunisia.
A new moderately halophilic sulfate-reducing bacterium (strain H₁(T) ) was enriched and isolated from a wastewater digestor in Tunisia. Cells were curved, motile rods (2-3 x 0.5 μm). Strain H₁(T) grew at temperatures between 22 and 43°C (optimum 35°C), and at pH between 5.0 and 9.2 (optimum 7.3-7.5). Strain H₁(T) required salt for growth (1-45 g of NaCl/l), with an optimum at 20-30 g/l. Sulfate, sulfite, thiosulfate, and elemental sulfur were used as terminal electron acceptors but not nitrate and nitrite. Strain H₁(T) utilized lactate, pyruvate, succinate, fumarate, ethanol, and hydrogen (in the presence of acetate and CO₂) as electron donors in the presence of sulfate as electron acceptor. The main end-products from lactate oxidation were acetate with H₂ and CO₂. The G + C content of the genomic DNA was 55%. The predominant fatty acids of strain H₁(T) were C(15:0) iso (38.8%), C(16:0) (19%), and C(14:0) iso 3OH (12.2%), and menaquinone MK-6 was the major respiratory quinone. Phylogenetic analysis of the small-subunit (SSU) ribosomal RNA (rRNA) gene sequence indicated that strain H₁(T) was affiliated to the genus Desulfovibrio. On the basis of SSU rRNA gene sequence comparisons and physiological characteristics, strain H₁(T) is proposed to be assigned to a novel species of sulfate reducers of the genus Desulfovibrio, Desulfovibrio legallis sp. nov. (= DSM 19129(T) = CCUG 54389(T)).
Thabet
Olfa Ben Dhia
OB
INSAT, Laboratoire d'Ecologie et de Technologie Microbienne, Centre Urbain Nord, BP 676, 1080, Tunis Cedex, Tunisia.
Wafa
Terres
T
Eltaief
Khelifi
K
Cayol
Jean-Luc
JL
Hamdi
Moktar
M
Fauque
Guy
G
Fardeau
Marie-Laure
ML
D015373:Bacterial Typing Techniques; D001482:Base Composition; D002264:Carboxylic Acids / Q000378:metabolism; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D003901:Desulfovibrio / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D005227:Fatty Acids / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology*; D012965:Sodium Chloride / Q000378:metabolism*; D013431:Sulfates / Q000378:metabolism*; D013447:Sulfites / Q000378:metabolism; D013455:Sulfur / Q000378:metabolism; D013696:Temperature; D014416:Tunisia
D002264:Carboxylic Acids; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D013431:Sulfates; D013447:Sulfites; D012965:Sodium Chloride; D013455:Sulfur
Arch Microbiol. 1981 Jul;129(5):395-400
7283636
Adv Appl Microbiol. 2009;68:41-98
19426853
Can J Microbiol. 1999 Feb;45(2):145-52
10380647
Anal Biochem. 1977 Aug;81(2):461-6
907108
Nature. 1959 Feb 14;183(4659):481-2
13632776
J Clin Microbiol. 2005 Aug;43(8):4041-5
16081948
Nucleic Acids Res. 1999 Jan 1;27(1):12-7
9847132
Oral Microbiol Immunol. 2002 Oct;17(5):321-3
12354215
J Bacteriol. 1990 Jul;172(7):3609-19
2361938
J Hazard Mater. 2009 Aug 15;167(1-3):1133-40
19272702
Syst Appl Microbiol. 2008 Mar;31(1):38-42
18221850
Int J Syst Evol Microbiol. 2002 Jul;52(Pt 4):1305-8
12148644
Nucleic Acids Res. 2001 Jan 1;29(1):173-4
11125082
Mol Biol Evol. 1987 Jul;4(4):406-25
3447015
Int J Syst Evol Microbiol. 2009 Dec;59(Pt 12):3100-4
19643880
Int J Syst Bacteriol. 1997 Oct;47(4):1013-9
9336900