title: Tumour necrosis factor-alpha gene polymorphisms and susceptibility to oral lichen planus.
abstract: OBJECTIVE
  This study is aimed to investigate the association between OLP susceptibility and clinical type in the Thai population and three polymorphisms within the promoter region of the TNF-α at positions -863, -308 and -238 which have putative functional significances.
  
  
  METHODS
  Genomic DNA from 75 Thai patients with OLP and 154 healthy controls were genotyped for TNF-α polymorphisms-- -863(rs1800630), -308(rs1800629), and -238(rs361525)--using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).
  
  
  RESULTS
  We found a higher proportion of TNF-alpha-308 AA genotype (high producer genotype) among OLP patients (5/75; 6.67%) when compared to healthy controls (1/154; 0.65%; OR = 10.93; 95% CI = 1.21-251.9). For other polymorphisms (-863 and -238), we did not find any significant association with OLP development; this was also the case with haplotype analysis (-863/-308/-238).
  
  
  CONCLUSIONS
  TNF-α-308AA may play a relevant role in the susceptibility and severity of OLP in the Thai population. However, further investigation of this study is needed.
authors.affiliation: Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok, Thailand.
authors.forename: I
authors.initials: I
authors.lastname: Kimkong
authors.forename: N
authors.initials: N
authors.lastname: Hirankarn
authors.forename: J
authors.initials: J
authors.lastname: Nakkuntod
authors.forename: N
authors.initials: N
authors.lastname: Kitkumthorn
mesh_terms: D000225:Adenine; D000328:Adult; D003596:Cytosine; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D006147:Guanine; D006239:Haplotypes; D006801:Humans; D017676:Lichen Planus, Oral / Q000145:classification / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length / Q000235:genetics; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D013785:Thailand; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*
chemical_list: D014409:Tumor Necrosis Factor-alpha; D006147:Guanine; D003596:Cytosine; D000225:Adenine