Downregulation of miR-125b in metastatic cutaneous malignant melanoma.
This study aimed to identify microRNA species involved in the earliest metastatic event in cutaneous malignant melanoma (MM). Samples from 28 patients with MM [stage T2 (tumor), M0 (distant metastasis)] were grouped by the presence of micrometastasis in the sentinel lymph nodes (N0/N1). Melanoma cells were harvested from primary, cutaneous MM tumors by laser-capture microdissection, and microRNA expression profiles were obtained by the microarray technique. Results were validated by quantitative reverse transcription PCR. We found that miR-125b was downregulated in the primary cutaneous melanomas that produced early metastases (T2, N1, M0) compared with the sentinel lymph node-negative (T2, N0, M0) melanomas. MiR-125b has earlier been found to be downregulated in other tumor types and in atypic naevi compared with the common acquired naevi. In conclusion, miR-125b may be involved in an early progression of cutaneous MM.
Glud
Martin
M
Department of Plastic Surgery and Burn Unit, Epigenomisk Laboratory, Rigshospitalet, University of Copenhagen, Denmark. glud.martin@gmail.com
Rossing
Maria
M
Hother
Christoffer
C
Holst
Line
L
Hastrup
Nina
N
Nielsen
Finn C
FC
Gniadecki
Robert
R
Drzewiecki
Krzysztof T
KT
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015536:Down-Regulation; D005260:Female; D006801:Humans; D008297:Male; D008545:Melanoma / Q000235:genetics* / Q000601:surgery; D035683:MicroRNAs / Q000235:genetics*; D046228:Microarray Analysis; D008875:Middle Aged; D009362:Neoplasm Metastasis; D012878:Skin Neoplasms / Q000235:genetics* / Q000601:surgery
C531888:MIRN125 microRNA, human; D035683:MicroRNAs