Partial purification and characterization of the mode of action of enterocin S37: a bacteriocin produced by Enterococcus faecalis S37 isolated from poultry feces.
The aim of this research was to purify and characterize the mode of action of enterocin S37, a bacteriocin produced by Enterococcus faecalis S37, a strain recently isolated from the chicken feces. Enterocin S37 has a molecular weight comprised between 4 and 5 kDa. It remained active after 1 h at 80(o)C and at pH values ranging from 4.0 to 9.0. Furthermore, cell-free supernatant of Enterococcus faecalis S37 and purified enterocin S37 were active against Gram-positive bacteria including Listeria monocytogenes EGDe, L. innocua F, Enterococcus faecalis JH2-2, and Lactobacillus brevis F145. The purification of enterocin S37 was performed by ammonium sulfate precipitation followed up by hydrophobic-interaction chromatography procedures. Treatment of enterocin S37 with proteinase K, alpha-chymotrypsin, and papain confirmed its proteinaceous nature, while its treatment with lysozyme and lipase resulted in no alteration of activity. Enterocin S37 is hydrophobic, anti-Listeria and likely acting by depletion of intracellular K(+) ions upon action on K(ATP) channels. This study contributed to gain more insights into the mode of action of enterocins.
Belguesmia
Y
Y
ONIRIS. Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique, Rue de la Géraudière, BP 82225, 44322 Nantes Cedex 3, France.
Choiset
Y
Y
Prévost
H
H
Dalgalarrondo
M
M
Chobert
J-M
JM
Drider
D
D
D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D001430:Bacteriocins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D002645:Chickens / Q000382:microbiology; D024881:Drug Resistance, Bacterial; D013293:Enterococcus faecalis / Q000302:isolation & purification / Q000378:metabolism*; D005243:Feces / Q000382:microbiology; D006094:Gram-Positive Bacteria / Q000187:drug effects
D000900:Anti-Bacterial Agents; D001430:Bacteriocins; C571347:enterocin S37, Enterococcus faecalis
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