title: The Corynebacterium glutamicum aconitase repressor: scratching around for crystals.
abstract: Imperfections on the surfaces of crystallization containers are known to influence crystal formation and are thought to do so by helping to overcome the nucleation barrier. The intentional creation of imperfections has been widely applied to induce crystallization of small molecules, but has not been reported for protein crystallization. Here, the crystallization and preliminary X-ray analysis of the TetR-type aconitase repressor are reported. This regulator was the first transcription factor to be identified in the regulation of the tricarboxylic acid cycle in Corynebacterium glutamicum, an organism that is of special industrial interest and is an emerging model organism for Corynebacterineae. Successful crystallization involved introducing manual scratches on the surface of standard commercial plates, which led to a substantial improvement in crystal nucleation and quality.
authors.affiliation: Structural Biology Laboratory, Department of Chemistry, University of York, York YO10 5DD, England.
authors.forename: Javier
authors.initials: J
authors.lastname: García-Nafría
authors.forename: Meike
authors.initials: M
authors.lastname: Baumgart
authors.forename: Michael
authors.initials: M
authors.lastname: Bott
authors.forename: Anthony J
authors.initials: AJ
authors.lastname: Wilkinson
authors.forename: Keith S
authors.initials: KS
authors.lastname: Wilson
mesh_terms: D000154:Aconitate Hydratase / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D048230:Corynebacterium glutamicum / Q000737:chemistry* / Q000378:metabolism; D003460:Crystallization
chemical_list: D001426:Bacterial Proteins; D000154:Aconitate Hydratase
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