title: Observed and predicted reproduction of Ceriodaphnia dubia exposed to chloride, sulfate, and bicarbonate.
abstract: Chronic toxicities of Cl(-), SO(4) (2-), and HCO(3) (-) to Ceriodaphnia dubia were evaluated in low- and moderate-hardness waters using a three-brood reproduction test method. Toxicity tests of anion mixtures were used to determine interaction effects and to produce models predicting C. dubia reproduction. Effluents diluted with low- and moderate-hardness waters were tested with animals acclimated to low- and moderate-hardness conditions to evaluate the models and to assess the effects of hardness and acclimation. Sulfate was significantly less toxic than Cl(-) and HCO(3) (-) in both types of water. Chloride and HCO(3) (-) toxicities were similar in low-hardness water, but HCO(3) (-) was the most toxic in moderate-hardness water. Low acute-to-chronic ratios indicate that toxicities of these anions will decrease quickly with dilution. Hardness significantly reduced Cl(-) and SO(4) (2-) toxicity but had little effect on HCO(3) (-). Chloride toxicity decreased with an increase in Na(+) concentration, and HCO(3) (-) toxicity may have been reduced by the dissolved organic carbon in effluent. Multivariate models using measured anion concentrations in effluents with low to moderate hardness levels provided fairly accurate predictions of reproduction. Determinations of toxicity for several effluents differed significantly depending on the hardness of the dilution water and the hardness of the water used to culture test animals. These results can be used to predict the contribution of elevated anion concentrations to the chronic toxicity of effluents; to identify effluents that are toxic due to contaminants other than Cl(-), SO(4) (2-), and HCO(3) (-); and to provide a basis for chemical substitutions in manufacturing processes.
authors.affiliation: U.S. Geological Survey, Patuxent Wildlife Research Center, Warnell School of Forestry and Natural Resources, University of Georgia, Athens, Georgia 30602, USA.
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Lasier
authors.affiliation: Textile Science Program, College of Family and Consumer Sciences, University of Georgia, Athens, Georgia 30602, USA.
authors.forename: Ian R
authors.initials: IR
authors.lastname: Hardin
mesh_terms: D000818:Animals; D001639:Bicarbonates / Q000633:toxicity*; D002712:Chlorides / Q000633:toxicity*; D033361:Cladocera / Q000187:drug effects* / Q000502:physiology; D012098:Reproduction / Q000187:drug effects; D013431:Sulfates / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D001639:Bicarbonates; D002712:Chlorides; D013431:Sulfates; D014874:Water Pollutants, Chemical
references.citation: Goodfellow WL, Ausley LW, Burton DT, Denton DL, Dorn PB, Grothe DR, Heber MA, Norberg-King TJ, Rodgers JH Jr. 2000. Major ion toxicity in effluents: a review with permitting recommendations. Environ Toxicol Chem 19: 175-182.
references.citation: McCulloch WL, Goodfellow WL, Black JA. 1993. Characterization, identification and confirmation of total dissolved solids as effluent toxicants. In Gorsuch JW, Dwyer FJ, Ingersoll CG, La Point TW, eds, Environmental Toxicology and Risk Assessment, Vol 2. STP 1216. American Society for Testing and Materials, Philadelphia, PA, pp 213-227.
references.citation: Chapman PM, Bailey H, Canaria E. 2000. Toxicity of total dissolved solids associated with two mine effluents to chironomid larvae and early life stages of rainbow trout. Environ Toxicol Chem 19: 210-214.
references.citation: Mount DR, Gulley DD, Hockett JR, Garrison TD, Evans JM. 1997. Statistical models to predict the toxicity of major ions to Ceriodaphnia dubia, Daphnia magna and Pimephales promelas (fathead minnows). Environ Toxicol Chem 16: 2009-2019.
references.citation: Mensing TM, Faure G. 1998. Effect of wastewater on the concentrations of Mo, Zn, Cu, and Ni in Mill Creek at Marysville, Ohio. Ohio J Sci 98: 80-83.
references.citation: Anderson CB, Lewis GP, Sargent KA. 2004. Influence of wastewater-treatment effluent on concentrations and fluxes of solutes in the Bush River, South Carolina, during extreme drought conditions. Environ Geosci 11: 28-41.
references.citation: Lewis Gp, Mitchell JD, Anderson CB, Haney DC, Liao M, Sargent KA. 2007. Urban influences on stream chemistry and biology in the Big Brushy Creek watershed, South Carolina. Water Air Soil Pollut 182: 303-323.
references.citation: Soucek DJ, Kennedy AJ. 2005. Effects of hardness, chloride, and acclimation on the acute toxicity of sulfate to freshwater invertebrates. Environ Toxicol Chem 24: 1204-1210.
references.citation: DeGraeve GM, Cooney JD, Marsh BH, Pollock TL, Reichenbach NG. 1992. Variability in the performance of the 7-d Ceriodaphnia dubia survival and reproduction test: an intra- and interlaboratory study. Environ Toxicol Chem 11: 851-866.
references.citation: Aragao MA, Pereira EV. 2003. Sensitivity of Ceriodaphnia dubia of different ages to sodium chloride. Bull Environ Contam Toxicol 70: 1247-1250.
references.citation: Harmon SM, Specht WL, Chandler GT. 2003. A comparison of the daphnids Ceriodaphnia dubia and Daphnia ambigua for their utilization in routine toxicity testing in the Southeastern United States. Arch Environ Contam Toxicol 45: 79-85.
references.citation: Lasier PJ, Winger PV, Hardin IR. 2006. Effects of hardness and alkalinity in culture and test waters on reproduction of Ceriodaphnia dubia. Environ Toxicol Chem 25: 2781-2786.
references.citation: U.S. Environmental Protection Agency. 2002. Short-term methods for estimating the chronic toxicity of effluents and receiving waters to freshwater organisms, 4th ed. EPA/821/R-02-013. Washington, DC.
references.citation: Kennedy AJ, Cherry DS, Zipper CE. 2005. Evaluation of ionic contribution to the toxicity of a coal-mine effluent using Ceriodaphnia dubia. Arch Environ Contam Toxicol 49: 155-162.
references.citation: Goto-Doshida S, Saito M, Nagayama M. 2003. Effects of calcium present in fabric and in washing liquor on detergency. J Oleo Sci 52: 273-282.
references.citation: Greenleaf J, Sengupta A. 2006. Environmentally benign hardness removal using ion exchange fibers and snowmelt. Environ Sci Technol 40: 370-376.
references.citation: Hou M, Baughman GL. 1992. Predicting the precipitation of acid and direct dyes in natural waters. Dyes Pigments 18: 35-46.
references.citation: Hem JD. 1985. Study and interpretation of the chemical characteristics of natural water. Water-Supply Paper 2254. U.S. Geological Survey, Washington, DC.
references.citation: American Public Health Association, American Water Works Association, and Water Environment Federation. 1992. Standard Methods for the Examination of Water and Wastewater, 18th ed. Washington, DC.
references.citation: Stumm W, Morgan JJ. 1981. Aquatic Chemistry, 2nd ed. John Wiley & Sons, New York, NY, USA.
references.citation: Norberg-King TJ. 1993. A linear interpolation method for sublethal toxicity: the inhibition concentration (ICp) approach. National Effluent Toxicity Assessment Center Technical Report 600/4-89-001 and 001A. U.S. Environmental Protection Agency, Duluth, MN.
references.citation: Steel RGD, Torrie JH. 1980. Principles and Procedures of Statistics: A Biometrical Approach, 2nd ed. McGraw-Hill, New York, NY, USA.
references.citation: Akaike H. 1974. A new look at the statistical model identification. IEEE Transact Autom Control 19: 716-723.
references.citation: Rand GM, Wells PG, McCarty LS. 1995. Introduction to aquatic toxicology. In Rand GM, ed, Fundamentals of Aquatic Toxicology, 2nd ed. Taylor & Francis, Washington, DC, pp 3-70.
references.citation: Bailey HC, Krassoi R, Elphick JR, Mulhall AM, Hunt P, Tedmanson L, Lovell A. 2000. Application of Ceriodaphnia dubia for whole effluent toxicity tests in the Hawkesbury Nepean watershed, New South Wales, Australia: Method development and validation. Environ Toxicol Chem 19: 88-93.
references.citation: Camargo JA, Alonso A, Salamanca A. 2005. Nitrate toxicity to aquatic animals: a review with new data for freshwater invertebrates. Chemosphere 58: 1255-1267.
references.citation: Soucek DJ. 2007. Bioenergetic effects of sodium sulfate on the freshwater crustacean. Ceriodaphnia dubia. Ecotoxicology 16: 317-325.
references.citation: Soucek DJ. 2007. Comparison of hardness- and chloride-regulated acute effects of sodium sulfate on two freshwater crustaceans. Environ Toxicol Chem 26: 773-779.
references.citation: U.S. Environmental Protection Agency. 1991. Technical support document for water quality-based toxics control. EPA/505/2-90-001. Washington, DC.
references.citation: Ahearn GA, Duerr JM, Zhuang Z, Brown RJ, Aslamkhan A, Killebrew DA. 1999. Ion transport processes of crustacean epithelial cells. Physiol Biochem Zool 72: 1-18.
references.citation: Perry SF, Shahsavarani A, Georgalis T, Bayaa M, Furimsky M, Thomas SLY. 2003. Channels, pumps, and exchangers in the gill and kidney of freshwater fishes: their role in ionic and acid-base regulation. J Exp Zool 300A: 53-62.
references.citation: Freidig AP, Garicano EA, Busser FJM, Hermens JLM. 1998. Estimating impact of humic acid on bioavailability and bioaccumulation of hydrophobic chemicals in guppies using kinetic solid-phase extraction. Environ Toxicol Chem 17: 998-1004.
references.citation: Kim SD, Ma H, Allen HE, Cha DK. 1999. Influence of dissolved organic matter on the toxicity of copper to Ceriodaphnia dubia: effect of complexation kinetics. Environ Toxicol Chem 18: 2433-2437.
references.citation: Akkanen J, Kukkonen JVK. 2003. Measuring the bioavailability of two hydrophobic organic compounds in the presence of dissolved organic matter. Environ Toxicol Chem 22: 518-524.
references.citation: Environment Canada. 2007. Biological test method: Test of reproduction and survival using the cladoceran Ceriodaphnia dubia, 2nd ed. EPS 1/RM/21. Environmental Science and Technology Centre, Ottawa, ON.