Daily bisphenol A excretion and associations with sex hormone concentrations: results from the InCHIANTI adult population study.
BACKGROUND
Bisphenol A (BPA) is a high production volume chemical widely used in packaging for food and beverages. Numerous studies have demonstrated that BPA can alter endocrine function in animals, yet human studies remain limited.


OBJECTIVE
We estimated daily excretion of BPA among adults and examined hypothesized associations with serum estrogen and testosterone concentrations.


METHODS
We conducted cross-sectional analyses using data from the InCHIANTI Study, a prospective population-based study of Italian adults. Our study included 715 adults between 20 and 74 years old. BPA concentrations were measured by liquid chromatography-mass spectrometry in 24-hr urine samples. The main outcome measures were serum concentrations of total testosterone and 17beta-estradiol.


RESULTS
Geometric mean urinary BPA concentration was 3.59 ng/mL [95% confidence interval (CI), 3.42-3.77 ng/mL], and mean excretion was 5.63 microg/day (5th population percentile, 2.1 microg/day; 95th percentile, 16.4 microg/day). We found higher excretion rates among men, younger respondents, and those with increasing waist circumference (p = 0.013) and weight (p = 0.003). Higher daily BPA excretion was associated with higher total testosterone concentrations in men, in models adjusted for age and study site (p = 0.044), and in models additionally adjusted for smoking, measures of obesity, and urinary creatinine concentrations (beta = 0.046; 95% CI, 0.015-0.076; p = 0.004). We found no associations with the other serum measures. We also found no associations with the primary outcomes among women, but we did find an association between BPA and SHBG concentrations in the 60 premenopausal women.


CONCLUSIONS
Higher BPA exposure may be associated with endocrine changes in men. The mechanisms involved in the observed cross-sectional association with total testosterone concentrations need to be clarified.
Galloway
Tamara
T
School of Biosciences, University of Exeter, Exeter, United Kingdom.
Cipelli
Riccardo
R
Guralnik
Jack
J
Ferrucci
Luigi
L
Bandinelli
Stefania
S
Corsi
Anna Maria
AM
Money
Cathryn
C
McCormack
Paul
P
Melzer
David
D
D000328:Adult; D000368:Aged; D001559:Benzhydryl Compounds; D003404:Creatinine / Q000652:urine; D003430:Cross-Sectional Studies; D052244:Endocrine Disruptors / Q000652:urine*; D004781:Environmental Exposure / Q000032:analysis* / Q000706:statistics & numerical data; D004967:Estrogens / Q000097:blood*; D005260:Female; D006801:Humans; D007558:Italy; D008297:Male; D008875:Middle Aged; D010636:Phenols / Q000652:urine*; D011446:Prospective Studies; D013739:Testosterone / Q000097:blood*; D055815:Young Adult; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D003404:Creatinine; D052244:Endocrine Disruptors; D004967:Estrogens; D010636:Phenols; D013739:Testosterone; C006780:bisphenol A; D000098826:Bisphenol A Compounds
Occup Environ Med. 2002 Sep;59(9):625-8
12205237
Environ Health Perspect. 2007 Dec;115 Suppl 1:69-76
18174953
Microsc Res Tech. 2009 Nov;72(11):773-86
19565636
Endocr J. 2004 Apr;51(2):165-9
15118266
Biochem Biophys Res Commun. 2002 Feb 15;291(1):76-8
11829464
J Clin Endocrinol Metab. 2004 Feb;89(2):525-33
14764757
Environ Health Perspect. 2005 Apr;113(4):391-5
15811827
Environ Health Perspect. 2010 Sep;118(9):1286-91
20494855
Environ Res. 2008 Oct;108(2):260-7
18774129
Toxicology. 2008 Apr 18;246(2-3):112-23
18294747
Endocrinology. 2004 Feb;145(2):592-603
14605012
Environ Health Perspect. 2009 May;117(5):784-9
19479022
Environ Health Perspect. 2008 Feb;116(2):173-8
18288314
Toxicol Lett. 2009 Sep 28;189(3):248-52
19539015
Toxicol Sci. 2005 Jun;85(2):823-38
15746009
JAMA. 2008 Sep 17;300(11):1303-10
18799442
J Clin Endocrinol Metab. 2002 Nov;87(11):5185-90
12414890
Environ Health Perspect. 2010 Aug;118(8):1055-70
20338858
J Am Geriatr Soc. 2000 Dec;48(12):1618-25
11129752
Environ Health Perspect. 2008 Jan;116(1):39-44
18197297
Int J Androl. 2008 Apr;31(2):194-200
17971160
Reprod Toxicol. 2007 Aug-Sep;24(2):139-77
17825522
Toxicol Appl Pharmacol. 2008 Apr 1;228(1):114-34
18207480
Reprod Toxicol. 2007 Aug-Sep;24(2):131-8
17768031
PLoS One. 2010 Jan 13;5(1):e8673
20084273
Philos Trans R Soc Lond B Biol Sci. 2009 Jul 27;364(1526):2079-96
19528057
Crit Rev Toxicol. 2009;39(1):1-75
18923974
Environ Health Perspect. 2005 Feb;113(2):192-200
15687057
Toxicol Sci. 2003 Sep;75(1):40-6
12805653
Environ Health Perspect. 2008 Jan;116(1):32-8
18197296
Environ Sci Technol. 2010 Feb 15;44(4):1458-63
20030380
Environ Health Prev Med. 2004 Jan;9(1):22-6
21432334
Endocrinology. 1997 Jul;138(7):2998-3005
9202245
N Engl J Med. 2010 Jul 8;363(2):109-22
20592293
Toxicol Sci. 2004 Nov;82(1):154-63
15282407
Arch Toxicol. 1998 Jun;72(7):387-94
9708877
Reprod Toxicol. 2007 Aug-Sep;24(2):199-224
17683900
Toxicol In Vitro. 2003 Aug;17(4):413-22
12849724
J Clin Endocrinol Metab. 1999 Oct;84(10):3666-72
10523012
Int J Androl. 2008 Apr;31(2):201-8
18315718
Reprod Toxicol. 2010 Apr;29(2):132-46
19931376
Mol Cell Endocrinol. 2008 Feb 13;283(1-2):12-8
18191889