title: Bioassay as monitoring system for lead phytoremediation through Crinum asiaticum L.
abstract: Toxicity of lead in soil is well documented and established. Phytoremediation has gained attention as a cheap, easily applicable, and eco-friendly clean-up technology. Chemical methods are used to assess exact levels and type of pollutants but heavy metal content in soil can also be evaluated indirectly by estimation of phytotoxicity levels using bioassays. Plant bioassays through fast germinating cereals can indicate not only the level of pollution and its effects on growth and survival but also the progress of phytoremediation process. The performance of barley Hordeum vulgare L. seedlings as bioassay for assessment of changes in the levels of lead (Pb) at three concentrations, i.e., 300 (T(1)), 600 (T(2)), and 1,200 ppm (T(3)) in the soil was evaluated while testing the efficiency of Crinum asiaticum L. as a phytoremedial tool. At the first assessment, i.e., 30 DAT (days after treatment) shoot and root lengths of seedlings decreased with increasing concentrations of Pb. As the study progressed, a decrease in levels of Pb was accompanied by better germinability and growth of barley. At 120 DAT seedling growth in all the treatments were comparable to control. In T(1), T(2), and T(3) soils, 74.5%, 83.7%, and 91.2% reduction in lead content was observed at 120 DAT. Highly significant correlations between decreasing pollutant (Pb) content in the soil, seed germination, and seedling growth of barley H. vulgare were found. The differences in root and shoot length as well as overall growth pattern are indicative of the suitability of barley as a bio-monitoring tool.
authors.affiliation: Department of Botany, St. John's College, Agra, 282002, India. 30mayank@gmail.com
authors.forename: Mayank
authors.initials: M
authors.lastname: Varun
authors.forename: Rohan
authors.initials: R
authors.lastname: D'Souza
authors.forename: Devendra
authors.initials: D
authors.lastname: Kumar
authors.forename: Manoj S
authors.initials: MS
authors.lastname: Paul
mesh_terms: D001673:Biodegradation, Environmental; D001681:Biological Assay; D031407:Crinum / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D004784:Environmental Monitoring / Q000379:methods*; D001467:Hordeum / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D007854:Lead / Q000378:metabolism* / Q000633:toxicity; D012987:Soil / Q000737:chemistry; D012989:Soil Pollutants / Q000378:metabolism* / Q000633:toxicity
chemical_list: D012987:Soil; D012989:Soil Pollutants; D007854:Lead
references.citation: Plant Physiol. 2000 Nov;124(3):1019-26
references.pmid: 11080279
references.citation: Environ Pollut. 2002;120(2):363-70
references.pmid: 12395850
references.citation: Plant Physiol. 1996 Mar;110(3):715-719
references.pmid: 12226213
references.citation: Sci Total Environ. 1991 Mar;100 Spec No:151-76
references.pmid: 2063180
references.citation: Annu Rev Plant Physiol Plant Mol Biol. 1998 Jun;49:643-668
references.pmid: 15012249