Antioxidant and hepatic protective effects of lotus root hot water extract with taurine supplementation in rats fed a high fat diet.
BACKGROUND
Nelumbo nucifera, known as sacred lotus, is a well-known medicinal plant and this lotus root is commonly used as food compared to different parts of this plant. This study was conducted to investigate the antioxidant and hepatic protective effects of lotus root hot water extract with taurine supplementation in high fat diet-induced obese rats.


METHODS
Thirty-two male Sprague-Dawley rats (4-week-old) were randomly divided into four groups (n=8) for 6 weeks (normal diet, N group; high fat diet, HF group; high fat diet + lotus root hot water extract, HFR group; high fat diet + lotus root hot water extract + taurine, HFRT group). Lotus root hot water extract was orally administrated (400 mg/kg/day) to HFR and HFRT groups and the same amount of distilled water was orally administered to N and HF groups. Taurine was supplemented by dissolving in feed water (3% w/v).


RESULTS
The activities of glutamate oxaloacetate transaminase and glutamate pyruvate transaminase in serum were lower in HFR and HFRT groups compared to HF group. Thiobarbituric acid reactive substance contents in all groups fed a high fat diet were higher compared to N group. The activities of hepatic antioxidant enzymes were higher in HFR and HFRT groups compared to HF group.


CONCLUSIONS
These results suggest that lotus root hot water extract with taurine supplementation shows antioxidant and hepatic protective effects in high fat diet-induced obese rats.
Du
Huan
H
Department of Food and Nutrition, Inha University, Incheon, Korea. sderic@gmail.com
Zhao
Xu
X
You
Jeong-Soon
JS
Park
Ji-Yeon
JY
Kim
Sung-Hoon
SH
Chang
Kyung-Ja
KJ
D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology*; D001219:Aspartate Aminotransferases / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D004041:Dietary Fats / Q000009:adverse effects*; D019587:Dietary Supplements; D005979:Glutathione Peroxidase / Q000378:metabolism; D006801:Humans; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D031653:Nelumbo / Q000033:anatomy & histology / Q000737:chemistry*; D009765:Obesity / Q000503:physiopathology; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000378:metabolism; D013654:Taurine / Q000008:administration & dosage / Q000494:pharmacology*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D000975:Antioxidants; D004041:Dietary Fats; D010936:Plant Extracts; D017392:Thiobarbituric Acid Reactive Substances; D013654:Taurine; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D001219:Aspartate Aminotransferases
Clin Biochem. 1999 Nov;32(8):595-603
10638941
Asia Pac J Clin Nutr. 2007;16 Suppl 1:158-63
17392096
Life Sci. 2006 Aug 8;79(11):1100-7
16624332
J Biol Chem. 1972 May 25;247(10):3170-5
4623845
J Basic Clin Physiol Pharmacol. 1995;6(3-4):205-28
8852268
J Clin Invest. 1955 Jan;34(1):126-31
13221663
Methods Enzymol. 1978;52:506-13
672654
Physiol Rev. 1992 Jan;72(1):101-63
1731369
Free Radic Res Commun. 1993;19(5):323-32
8314113
J Nutr. 1977 Jul;107(7):1340-8
874577
Methods Enzymol. 1978;52:302-10
672633
Redox Rep. 2004;9(2):105-10
15231065
Eur J Gastroenterol Hepatol. 2005 Sep;17(9):917-21
16093868
Biosci Biotechnol Biochem. 2002 Aug;66(8):1755-8
12353642
Curr Opin Clin Nutr Metab Care. 2006 Nov;9(6):728-33
17053427
Free Radic Biol Med. 1996;21(6):895-902
8902534
J Pharm Pharmacol. 2009 Apr;61(4):407-22
19298686
Methods Enzymol. 1984;105:198-209
6328179
Physiol Rev. 2004 Oct;84(4):1381-478
15383655
Prog Clin Biol Res. 1985;179:137-47
2997793