A general scheme for synthesis of substrate-based polyketide labels for acyl carrier proteins.
A general strategy to enzymatically label acyl carrier proteins (ACPs) of polyketide synthases has been developed. Incorporation of a chloromethyl ketone or vinyl ketone moiety into polyketide chain elongation intermediate mimics allows for the synthesis of CoA adducts. These CoA adducts undergo enzymatic reaction with Sfp, a phosphopantetheinyl transferase, to afford labeled CurB carrier proteins.
Leggans
Erick K
EK
Department of Medicinal Chemistry, University of Minnesota, 717 Delaware Street SE, Room 456, Minneapolis, MN 55414-2959, USA.
Akey
David L
DL
Smith
Janet L
JL
Fecik
Robert A
RA
D000213:Acyl Carrier Protein / Q000737:chemistry*; D001426:Bacterial Proteins / Q000378:metabolism; D003065:Coenzyme A / Q000737:chemistry; D007659:Ketones / Q000737:chemistry; D048630:Polyketide Synthases / Q000737:chemistry* / Q000378:metabolism; D017434:Protein Structure, Tertiary; D013379:Substrate Specificity; D017855:Transferases (Other Substituted Phosphate Groups) / Q000378:metabolism
D000213:Acyl Carrier Protein; D001426:Bacterial Proteins; D007659:Ketones; C082662:phosphopantetheinyl transferase; D048630:Polyketide Synthases; D017855:Transferases (Other Substituted Phosphate Groups); D003065:Coenzyme A
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