Expression of human apolipoprotein A-I in Nicotiana tabacum.
Several transgenic tobacco lines expressing human apolipoprotein A-I (ApoA-I) were obtained. Western blot analyses indicated the expression of the recombinant protein in plant organs at various stages of development, including senescent leaves. A cell line expressing human ApoA-I was established from a T(1) transgenic plant. Recombinant ApoA-I was isolated either from extracts of transgenic leaves and from the culture medium of transgenic cells using an antibody-based one-step procedure.
Chiaiese
Pasquale
P
Department of Soil, Plant, Environmental and Animal Production Sciences, School of Biotechnological Sciences, University of Naples Federico II, Naples, Italy. chiaiese@unina.it
Minutolo
Maria
M
Arciello
Angela
A
Guglielmi
Fulvio
F
Guglielmini
Fulvio
F
Piccoli
Renata
R
Filippone
Edgardo
E
D016632:Apolipoprotein A-I / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification; D015153:Blotting, Western; D018929:Cell Culture Techniques; D002846:Chromatography, Affinity; D015870:Gene Expression; D006801:Humans; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism*
C505693:APOA1 protein, human; D016632:Apolipoprotein A-I; D011994:Recombinant Proteins