title: [Effect of NAD+ against radiation injury and its dose-effect relationship].
abstract: OBJECTIVE
  To explore the effect of NAD+ against radiation injury and its dose-effect relationship.
  
  
  METHODS
  L02 liver cells cultured in RPMI 1640 medium containing 10% fetal calf serum were exposed to X-ray irradiation followed by immediate application of NAD+. The cellular viability was analyzed by MTT assay and the apoptotic cells were detected by TUNEL methods to observe the damages of L02 liver cells induced by X-ray exposure and analyze the dose-effect relationship of NAD+.
  
  
  RESULTS
  The viability of L02 liver cells was decreased with increasing dose of X-ray irradiation. The most obvious growth inhibition of L02 cells occurred 24 h after the irradiation. NAD+ significantly increased the cell survival rate after irradiation, and this effect was gradually increased within the concentration range of 100-1000 microg/ml; at higher concentrations, the survival rate of the irradiated L02 cells showed no significant increase.
  
  
  CONCLUSIONS
  NAD+ provides partial protection of the liver cells against radiation injury, and the effect is positively correlated to NAD+ concentration within a certain range.
authors.affiliation: Department of Radiotherapy, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. lmy.69@163.com
authors.forename: Min-ying
authors.initials: MY
authors.lastname: Li
authors.forename: Feng
authors.initials: F
authors.lastname: Lei
authors.forename: Wei
authors.initials: W
authors.lastname: You
authors.forename: Yu-jing
authors.initials: YJ
authors.lastname: Tan
authors.forename: Xiao-jun
authors.initials: XJ
authors.lastname: Lu
authors.forename: Long-hua
authors.initials: LH
authors.lastname: Chen
authors.forename: Ji-ren
authors.initials: JR
authors.lastname: Zhang
mesh_terms: D017209:Apoptosis / Q000187:drug effects* / Q000528:radiation effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects* / Q000528:radiation effects; D004305:Dose-Response Relationship, Drug; D022781:Hepatocytes / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects; D006801:Humans; D009243:NAD / Q000008:administration & dosage / Q000494:pharmacology*; D011832:Radiation Injuries / Q000517:prevention & control
chemical_list: D009243:NAD