Establishment of fruit bat cells (Rousettus aegyptiacus) as a model system for the investigation of filoviral infection.
BACKGROUND
The fruit bat species Rousettus aegyptiacus was identified as a potential reservoir for the highly pathogenic filovirus Marburg virus. To establish a basis for a molecular understanding of the biology of filoviruses in the reservoir host, we have adapted a set of molecular tools for investigation of filovirus replication in a recently developed cell line, R06E, derived from the species Rousettus aegyptiacus.


RESULTS
Upon infection with Ebola or Marburg viruses, R06E cells produced viral titers comparable to VeroE6 cells, as shown by TCID(50) analysis. Electron microscopic analysis of infected cells revealed morphological signs of filovirus infection as described for human- and monkey-derived cell lines. Using R06E cells, we detected an unusually high amount of intracellular viral proteins, which correlated with the accumulation of high numbers of filoviral nucleocapsids in the cytoplasm. We established protocols to produce Marburg infectious virus-like particles from R06E cells, which were then used to infect naïve target cells to investigate primary transcription. This was not possible with other cell lines previously tested. Moreover, we established protocols to reliably rescue recombinant Marburg viruses from R06E cells.


CONCLUSIONS
These data indicated that R06E cells are highly suitable to investigate the biology of filoviruses in cells derived from their presumed reservoir.
Krähling
Verena
V
Institut für Virologie, Philipps-Universität Marburg, Marburg, Germany.
Dolnik
Olga
O
Kolesnikova
Larissa
L
Schmidt-Chanasit
Jonas
J
Jordan
Ingo
I
Sandig
Volker
V
Günther
Stephan
S
Becker
Stephan
S
D000818:Animals; D002460:Cell Line; D002685:Chiroptera; D002522:Chlorocebus aethiops; D029043:Ebolavirus / Q000254:growth & development* / Q000472:pathogenicity; D029024:Marburgvirus / Q000254:growth & development* / Q000472:pathogenicity; D019562:Viral Load; D014773:Virology / Q000379:methods*; D014776:Virus Cultivation / Q000379:methods; D014779:Virus Replication
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