The expression profile and function of Satb2 in zebrafish embryonic development.
The present study shows the expression profile and function of the homeobox gene, satb2 during zebrafish embryonic development. Satb2 was ubiquitously expressed from the 1 cell stage to the 10-somite stage in zebrafish embryos. Satb2 showed stage-specific expression profiles such as in the pronephric duct at 24 hpf, the branchial arches at 36 hpf, and the ganglion cell layer of the retina and fins at 48 hpf. Additionally, satb2 knockdown embryos were arrested at 50-60% epiboly, and transplantation experiments with satb2 knockdown cells showed migration defects. Interestingly, satb2 knockdown cells also exhibited down-regulation of dynamin II and VAMP4, which are involved in exocytosis and endocytosis, respectively. Furthermore, satb2 knockdown cells have a disorganized actin distribution and an underdeveloped external yolk syncytial layer, both of which are involved in epiboly. These results suggest that satb2 has a functional role in epiboly. This role may potentially be the regulation of endo-exocytic vesicle transport-dependent cell migration and/or the regulation of the development of the yolk syncytial layer.
Ahn
Hyun-Jong
HJ
Department of Microbiology, Kyung Hee University Medical School, Seoul, 130-701, Korea.
Park
Yoojin
Y
Kim
Suhyun
S
Park
Hae-chul
HC
Seo
Su-Kyoung
SK
Yeo
Sang-Yeob
SY
Geum
Dongho
D
D000818:Animals; D001934:Branchial Region / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D002465:Cell Movement / Q000235:genetics; D015536:Down-Regulation; D034285:Dynamin II / Q000032:analysis; D004625:Embryo, Nonmammalian / Q000196:embryology / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D055785:Gene Knockdown Techniques; D005801:Genes, Homeobox; D017403:In Situ Hybridization; D036961:Matrix Attachment Region Binding Proteins / Q000235:genetics* / Q000378:metabolism*; D051379:Mice; D012165:Retinal Ganglion Cells / Q000166:cytology / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism*; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics* / Q000378:metabolism*; D029961:Zebrafish Proteins / Q000235:genetics* / Q000378:metabolism*
D036961:Matrix Attachment Region Binding Proteins; C555948:Satb2 protein, zebrafish; D014157:Transcription Factors; D029961:Zebrafish Proteins; D034285:Dynamin II