title: MORPHEUS' MOLECULE1 is required to prevent aberrant RNA transcriptional read-through in Arabidopsis.
abstract: Several pathways function to remove aberrant mRNA in eukaryotic cells; however, the exact mechanisms underlying the restriction of aberrant mRNA transcription are poorly understood. In this study, we found that MORPHEUS' MOLECULE1 (MOM1) is a key component of this regulatory machinery. The Arabidopsis (Arabidopsis thaliana) mom1-44 mutation was identified by luciferase imaging in transgenic plants harboring a cauliflower mosaic virus 35S promoter-LUCIFERASE transgene lacking the 3'-untranslated region. In the mom1-44 mutant, transcriptional read-though occurred in genes with an aberrant RNA structure. Analysis of an RNA-dependent RNA polymerase2 mom1 double mutant revealed that the RNA-directed DNA methylation pathway is not involved in this regulatory process. Moreover, the prevention of aberrant mRNA transcriptional read-through by MOM1 is gene locus and transgene copy number independent.
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhou
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.forename: Huixin
authors.initials: H
authors.lastname: Lin
authors.forename: Guangqin
authors.initials: G
authors.lastname: Guo
authors.forename: Yan
authors.initials: Y
authors.lastname: Guo
mesh_terms: D020413:3' Untranslated Regions; D000074183:ATPases Associated with Diverse Cellular Activities; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D019175:DNA Methylation; D018506:Gene Expression Regulation, Plant; D009154:Mutation; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D026723:Polyadenylation; D018749:RNA, Plant / Q000096:biosynthesis; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D020413:3' Untranslated Regions; D029681:Arabidopsis Proteins; D009687:Nuclear Proteins; D018749:RNA, Plant; D014157:Transcription Factors; D000074183:ATPases Associated with Diverse Cellular Activities; C409208:MOM protein, Arabidopsis
references.citation: EMBO Rep. 2006 Dec;7(12):1273-8
references.pmid: 17082821
references.citation: Nature. 2000 May 11;405(6783):203-6
references.pmid: 10821279
references.citation: Genes Dev. 1997 Nov 1;11(21):2755-66
references.pmid: 9353246
references.citation: FEMS Microbiol Rev. 1999 Jun;23(3):277-95
references.pmid: 10371034
references.citation: Genes Dev. 2007 Aug 1;21(15):1833-56
references.pmid: 17671086
references.citation: Plant Cell. 2001 Jun;13(6):1383-400
references.pmid: 11402167
references.citation: Trends Biochem Sci. 1989 Mar;14(3):105-10
references.pmid: 2658217
references.citation: Cell. 2002 Feb 22;108(4):501-12
references.pmid: 11909521
references.citation: Nat Protoc. 2006;1(6):3094-100
references.pmid: 17406505
references.citation: Plant Cell. 2000 Jul;12(7):1165-78
references.pmid: 10899982
references.citation: EMBO J. 2010 Jan 20;29(2):352-62
references.pmid: 20010696
references.citation: EMBO Rep. 2002 Oct;3(10):951-5
references.pmid: 12231508
references.citation: FEBS Lett. 2007 Jun 19;581(15):2845-53
references.pmid: 17531985
references.citation: Genome Biol. 2008;9(3):R48
references.pmid: 18315870
references.citation: Curr Biol. 2005 Nov 8;15(21):1919-25
references.pmid: 16271868
references.citation: Microbiol Mol Biol Rev. 1999 Jun;63(2):405-45
references.pmid: 10357856
references.citation: Proc Natl Acad Sci U S A. 2006 Oct 10;103(41):14994-5001
references.pmid: 17008405
references.citation: EMBO J. 1991 Nov;10(11):3513-22
references.pmid: 1833188
references.citation: Mol Cell. 2009 Feb 13;33(3):354-64
references.pmid: 19217409
references.citation: Plant Cell. 2005 Jun;17(6):1658-73
references.pmid: 15849273
references.citation: EMBO Rep. 2006 Dec;7(12):1279-84
references.pmid: 17082818
references.citation: EMBO J. 1986 Nov;5(11):2915-22
references.pmid: 3024968
references.citation: Results Probl Cell Differ. 2001;34:139-55
references.pmid: 11288673
references.citation: EMBO J. 2010 Jan 20;29(2):340-51
references.pmid: 19910926
references.citation: Science. 2003 Aug 1;301(5633):653-7
references.pmid: 12893945
references.citation: Plant Cell. 2009 Jul;21(7):2045-57
references.pmid: 19602621
references.citation: Annu Rev Biochem. 2007;76:51-74
references.pmid: 17352659
references.citation: Curr Opin Cell Biol. 2009 Jun;21(3):367-76
references.pmid: 19243928
references.citation: Plant Biotechnol J. 2010 Sep;8(7):772-82
references.pmid: 20331530
references.citation: Cell. 2009 Feb 20;136(4):688-700
references.pmid: 19239889
references.citation: Plant J. 2003 Feb;33(4):743-9
references.pmid: 12609046
references.citation: Plant Cell. 2007 Mar;19(3):943-58
references.pmid: 17384170
references.citation: Mol Cell Biol. 1996 Apr;16(4):1534-42
references.pmid: 8657127