Spindle and chromosome configurations of in vitro-matured oocytes from polycystic ovary syndrome and ovulatory infertile women: a pilot study.
OBJECTIVE
To evaluate the meiotic spindle and chromosomal distribution of in vitro-matured oocytes from infertile nonobese women with PCOS and male or tubal causes of infertility (controls), and to compare in vitro maturation (IVM) rates between groups.


METHODS
Seventy four patients (26 with PCOS and 48 controls) undergoing stimulated cycles of oocyte retrieval for ICSI were selected prospectively. Thirteen PCOS patients and 27 controls had immature oocytes retrieved submitted to IVM. After IVM, oocytes showing extrusion of the first polar body were fixed and processed for evaluation of the meiotic spindle and chromosome distribution by immunofluorescence microscopy.


RESULTS
There were no differences between PCOS and control groups with respect to IVM rates (50.0% and 42.9%, respectively) nor the percentage of meiotic abnormalities in metaphase II oocytes (35.3% and 25%, respectively).


CONCLUSIONS
In vitro-matured oocytes obtained from stimulated cycles of nonobese PCOS did not have an increased ratio of meiotic abnormalities.
Vieira
Rodolpho C
RC
Departamento de Ginecologia e Obstetrícia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, São Paulo, Brazil.
Barcelos
Ionara D
ID
Ferreira
Elisa M
EM
Martins
Wellington P
WP
Ferriani
Rui A
RA
Navarro
Paula A
PA
D000328:Adult; D016022:Case-Control Studies; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D005260:Female; D006801:Humans; D007247:Infertility, Female / Q000503:physiopathology*; D008540:Meiosis; D008856:Microscopy, Fluorescence; D054315:Oocyte Retrieval; D009865:Oocytes / Q000166:cytology*; D009866:Oogenesis; D010865:Pilot Projects; D011085:Polycystic Ovary Syndrome / Q000503:physiopathology*; D011446:Prospective Studies; D008941:Spindle Apparatus / Q000648:ultrastructure*
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