title: Comparative analysis of pistil transcriptomes reveals conserved and novel genes expressed in dry, wet, and semidry stigmas.
abstract: Fertilization in angiosperms depends on a complex cellular "courtship" between haploid pollen and diploid pistil. These pollen-pistil interactions are regulated by a diversity of molecules, many of which remain to be identified and characterized. Thus, it is unclear to what extent these processes are conserved among angiosperms, a fact confounded by limited sampling across taxa. Here, we report the analysis of pistil-expressed genes in Senecio squalidus (Asteraceae), a species from euasterid II, a major clade for which there are currently no data on pistil-expressed genes. Species from the Asteraceae characteristically have a "semidry stigma," intermediate between the "wet" and "dry" stigmas typical of the majority of angiosperms. Construction of pistil-enriched cDNA libraries for S. squalidus allowed us to address two hypotheses: (1) stigmas of S. squalidus will express genes common to wet and dry stigmas and genes specific to the semidry stigma characteristic of the Asteraceae; and (2) genes potentially essential for pistil function will be conserved between diverse angiosperm groups and therefore common to all currently available pistil transcriptome data sets, including S. squalidus. Our data support both these hypotheses. The S. squalidus pistil transcriptome contains novel genes and genes previously identified in pistils of species with dry stigmas and wet stigmas. Comparative analysis of the five pistil transcriptomes currently available (Oryza sativa, Crocus sativus, Arabidopsis thaliana, Nicotiana tabacum, and S. squalidus), representing four major angiosperm clades and the three stigma states, identified novel genes and conserved genes potentially regulating pollen-pistil interaction pathways common to monocots and eudicots.
authors.affiliation: School of Biological Sciences, University of Bristol, Bristol, United Kingdom.
authors.forename: Alexandra M
authors.initials: AM
authors.lastname: Allen
authors.forename: Christian
authors.initials: C
authors.lastname: Lexer
authors.forename: Simon J
authors.initials: SJ
authors.lastname: Hiscock
mesh_terms: D017360:Arabidopsis / Q000235:genetics; D055028:Comparative Genomic Hybridization; D027622:Crocus / Q000235:genetics; D035264:Flowers / Q000378:metabolism*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D012275:Oryza / Q000235:genetics; D018749:RNA, Plant / Q000235:genetics; D012674:Senecio / Q000235:genetics* / Q000378:metabolism; D016415:Sequence Alignment; D014026:Nicotiana / Q000235:genetics
chemical_list: D018749:RNA, Plant
references.citation: New Phytol. 2008 Jul;179(2):286-317
references.pmid: 19086285
references.citation: Plant J. 2000 Dec;24(6):725-34
references.pmid: 11135107
references.citation: Annu Rev Plant Biol. 2005;56:467-89
references.pmid: 15862104
references.citation: Plant Physiol. 2008 Jun;147(2):852-63
references.pmid: 18434608
references.citation: Cell. 1995 Aug 11;82(3):395-403
references.pmid: 7634329
references.citation: Plant Physiol. 2009 Mar;149(3):1211-30
references.pmid: 19052150
references.citation: Plant Cell. 2004;16 Suppl:S84-97
references.pmid: 15075396
references.citation: J Exp Bot. 2003 Jan;54(380):47-54
references.pmid: 12456754
references.citation: Trends Plant Sci. 2004 Jul;9(7):320-4
references.pmid: 15231276
references.citation: New Phytol. 2006;172(2):221-8
references.pmid: 16995910
references.citation: J Exp Bot. 2006;57(8):1835-46
references.pmid: 16698818
references.citation: Plant Physiol. 2005 May;138(1):153-60
references.pmid: 15821148
references.citation: Plant Physiol. 2007 Aug;144(4):1797-812
references.pmid: 17556504
references.citation: Plant Mol Biol. 2005 Mar;57(5):659-77
references.pmid: 15988562
references.citation: Plant Physiol. 2005 Nov;139(3):1389-400
references.pmid: 16244157
references.citation: Protoplasma. 2006 Aug;228(1-3):21-30
references.pmid: 16937051
references.citation: Nat Rev Genet. 2002 Feb;3(2):137-44
references.pmid: 11836507
references.citation: BMC Plant Biol. 2007 Oct 09;7:53
references.pmid: 17925031
references.citation: Biochem Biophys Res Commun. 2005 Mar 4;328(1):144-9
references.pmid: 15670762
references.citation: Plant J. 1993 Jan;3(1):151-60
references.pmid: 8401601
references.citation: Plant Cell. 1998 Mar;10(3):319-30
references.pmid: 9501107
references.citation: Plant Cell. 2002 Sep;14(9):2277-87
references.pmid: 12215520
references.citation: J Exp Bot. 2005 Feb;56(412):605-11
references.pmid: 15582929
references.citation: Trends Plant Sci. 2003 Mar;8(3):97-9
references.pmid: 12663216
references.citation: Plant Mol Biol. 2001 Jan;45(1):51-62
references.pmid: 11247606
references.citation: Heredity (Edinb). 2000 Jul;85 ( Pt 1):10-9
references.pmid: 10971686
references.citation: Cell Mol Life Sci. 2001 Sep;58(10):1418-29
references.pmid: 11693523
references.citation: J Exp Bot. 2003 Jan;54(380):169-74
references.pmid: 12456767
references.citation: Plant Physiol. 2005 Jun;138(2):977-89
references.pmid: 15894741
references.citation: Plant Cell. 1998 Oct;10(10):1623-36
references.pmid: 9761790
references.citation: Plant J. 2004 Aug;39(3):343-53
references.pmid: 15255864
references.citation: Plant Cell. 2005 Jul;17(7):2009-19
references.pmid: 15937228
references.citation: Plant Cell. 1994 Jan;6(1):11-23
references.pmid: 12244218
references.citation: Plant J. 2005 Sep;43(5):716-23
references.pmid: 16115068
references.citation: Plant Physiol. 2005 Jul;138(3):1334-46
references.pmid: 15951488
references.citation: J Biol Chem. 2007 Nov 16;282(46):33845-33858
references.pmid: 17878166
references.citation: Proc Natl Acad Sci U S A. 2000 Mar 28;97(7):3765-70
references.pmid: 10716697
references.citation: Theor Appl Genet. 2006 Feb;112(3):455-61
references.pmid: 16328230
references.citation: Plant Physiol. 2006 Dec;142(4):1397-411
references.pmid: 16998086
references.citation: J Exp Bot. 2004 Aug;55(403):1643-54
references.pmid: 15258165
references.citation: Annu Rev Genet. 2004;38:793-818
references.pmid: 15568994
references.citation: Plant Cell. 1998 Aug;10(8):1333-47
references.pmid: 9707533
references.citation: J Exp Bot. 2003 Jan;54(380):55-63
references.pmid: 12456755
references.citation: Heredity (Edinb). 2011 Jan;106(1):113-23
references.pmid: 20372180
references.citation: Plant Cell. 2004;16 Suppl:S98-106
references.pmid: 15010514
references.citation: Planta. 2006 Mar;223(4):736-45
references.pmid: 16208487
references.citation: Proc Natl Acad Sci U S A. 2006 Jul 5;103(27):10503-10508
references.pmid: 16798873
references.citation: Plant Mol Biol. 2003 Jan;51(2):183-9
references.pmid: 12602877
references.citation: Plant Physiol. 2003 Oct;133(2):713-25
references.pmid: 14500793