title: Effect of poly(3-octylthiophene) doping on the attachment and proliferation of osteoblasts.
abstract: The effect of doping P3OT with ferric chloride on the attachment and proliferation of MC3T3-E1 osteoblasts is reported. Cell density and area correlated strongly with doping concentration: cells were larger and exhibited better spreading as doping increased. Cells cultured on undoped P3OT showed a decrease in proliferation between 24 and 48 h followed by a recovery after 72 h. However, this trend diminished with increasing doping concentration, and disappeared completely at the highest dopant level investigated. Analysis of cell-cell spatial distributions suggested that contact inhibition of proliferation occurred similarly on both undoped and doped P3OT. From these results, FeCl(3)-doping had no significant deleterious effect on attachment or proliferation of osteoblasts in vitro.
authors.affiliation: School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA.
authors.forename: Charlene
authors.initials: C
authors.lastname: Rincón
authors.forename: Chien-Chiang
authors.initials: CC
authors.lastname: Chen
authors.forename: J Carson
authors.initials: JC
authors.lastname: Meredith
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects* / Q000502:physiology; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects*; D002712:Chlorides / Q000494:pharmacology*; D003260:Contact Inhibition / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D005290:Ferric Compounds / Q000494:pharmacology*; D051379:Mice; D008856:Microscopy, Fluorescence; D010006:Osteoblasts / Q000502:physiology*; D011108:Polymers / Q000494:pharmacology*; D013876:Thiophenes / Q000494:pharmacology*
chemical_list: D002712:Chlorides; D005290:Ferric Compounds; D011108:Polymers; D013876:Thiophenes; C088351:poly(3-octylthiophene); C024555:ferric chloride