title: Phage display as a novel screening tool for primary toxicological targets.
abstract: In the present study the use of phage display as a screening tool to determine primary toxicological targets was investigated. These primary toxicological targets are the targets in the cell with which a chemical compound initially interacts and that are responsible for consecutive (toxic) effects. Nickel was used as model compound for the present study. By selection of Ni-binding peptides out of a 12-mer peptide phage library, it was possible to identify primary toxicological targets of Ni (and other metals). The selected Ni-binding peptides showed similarities to important primary toxicological targets of Ni, such as the hydrogenase nickel incorporation protein (hypB) and the Mg/Ni/Co transporter (corA). This shows that phage display, which is already widely used in other research fields, also has potential in ecotoxicology, as a novel screening tool with which to determine primary toxicological targets of chemical compounds.
authors.affiliation: Department of Biology, Laboratory for Ecophysiology, Biochemistry and Toxicology, University Antwerp, Groenenborgerlaan 171, B-2020 Antwerp, Belgium.|Institute for Agricultural and Fisheries Research (ILVO), Ankerstraat 1, B-8400 Oostende, Belgium.
authors.forename: Bieke
authors.initials: B
authors.lastname: Van Dorst
authors.affiliation: Department of Biology, Laboratory for Ecophysiology, Biochemistry and Toxicology, University Antwerp, Groenenborgerlaan 171, B-2020 Antwerp, Belgium.|European Chemicals Agency (ECHA), Annankatu 18, F-00120 Helsinki, Finland.
authors.forename: Wim
authors.initials: W
authors.lastname: De Coen
authors.affiliation: Department of Biology, Laboratory for Ecophysiology, Biochemistry and Toxicology, University Antwerp, Groenenborgerlaan 171, B-2020 Antwerp, Belgium.
authors.forename: Ronny
authors.initials: R
authors.lastname: Blust
authors.affiliation: Department of Biology, Laboratory for Ecophysiology, Biochemistry and Toxicology, University Antwerp, Groenenborgerlaan 171, B-2020 Antwerp, Belgium.|Institute for Agricultural and Fisheries Research (ILVO), Ankerstraat 1, B-8400 Oostende, Belgium.
authors.forename: Johan
authors.initials: J
authors.lastname: Robbens
mesh_terms: D019295:Computational Biology; D030562:Databases, Protein; D004797:Enzyme-Linked Immunosorbent Assay; D009532:Nickel / Q000378:metabolism / Q000633:toxicity*; D019151:Peptide Library; D011485:Protein Binding; D018675:Toxicity Tests / Q000379:methods*
chemical_list: D019151:Peptide Library; D009532:Nickel
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