High-speed RNA microextraction technology using magnetic oligo-dT beads and lateral magnetophoresis.
This paper presents a high-speed RNA microextractor for the direct isolation of RNA from peripheral blood lysate using magnetic oligo-dT beads. The extraction is achieved through lateral magnetophoresis, generated by a ferromagnetic wire array inlaid on a glass substrate. This RNA microextractor separated more than 80% of magnetic beads with a flow rate up to 20 ml h(-1), and the overall extraction procedure was completed within 1 min. The absorbance ratio of RNA to protein (A(260)/A(280)) was >1.7, indicating that the extraction technology yielded nearly pure RNA. The feasibility of this technique was evaluated further for its applicability to reverse transcription polymerase chain reaction (RT-PCR) procedures by performing cDNA synthesis and PCR. The analysis verified that the RNA microextractor is a practical method for easy, rapid, and high-precision RT-PCR using minimal reagent volumes without requiring highly trained personnel. In addition, it can be readily incorporated into genetic analysis procedures for realizing automated on-chip genetic platforms in a micro format.
Lee
Hwanyong
H
School of Nano Engineering, Inje University, 607 Obang-dong, Gimhae, GyongNam, 621-749, Republic of Korea.
Jung
Jinhee
J
Han
Song-I
SI
Han
Ki-Ho
KH
D001709:Biotechnology / Q000295:instrumentation*; D001774:Blood Chemical Analysis / Q000295:instrumentation*; D004586:Electrophoresis / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D008280:Magnetics / Q000295:instrumentation*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D008846:Micromanipulation / Q000295:instrumentation*; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D012313:RNA / Q000097:blood* / Q000737:chemistry
D009838:Oligodeoxyribonucleotides; C027903:oligo (dT); D012313:RNA