Expression, purification and crystallization of Chaetomium thermophilum Cu,Zn superoxide dismutase.
Cu,Zn superoxide dismutase (Cu,ZnSOD) from the thermophilic fungus Chaetomium thermophilum was expressed in Pichia pastoris and purified. Crystals were grown in over 120 conditions but only those produced with 1.4 M sodium potassium phosphate pH 8.2 as precipitant were suitable for structural studies. Data were collected to 1.9 A resolution at 100 K from a single crystal using a synchrotron-radiation source. The crystals belonged to space group P6(1)/P6(5), with unit-cell parameters a=90.2, c=314.5 A and eight molecules in the asymmetric unit. Elucidation of the crystal structure will provide insights into the active site of the enzyme and a better understanding of the structure-activity relationship, assembly and thermal stability of Cu,ZnSODs.
Wakadkar
Sachin
S
Turku Centre for Biotechnology, University of Turku and Abo Akademi, Turku 20521, Finland.
Zhang
Li-Qing
LQ
Li
Duo-Chuan
DC
Haikarainen
Teemu
T
Dhavala
Prathusha
P
Papageorgiou
Anastassios C
AC
D002597:Chaetomium / Q000201:enzymology*; D003460:Crystallization; D018360:Crystallography, X-Ray; D015870:Gene Expression; D013482:Superoxide Dismutase / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification
D013482:Superoxide Dismutase
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