title: Crystallographic study of wild-type carbonic anhydrase alpha CA1 from Chlamydomonas reinhardtii.
abstract: Carbonic anhydrases (CAs) are ubiquitously distributed and are grouped into three structurally independent classes (alphaCA, betaCA and gammaCA). Most alphaCA enzymes are monomeric, but alphaCA1 from Chlamydomonas reinhardtii is a dimer that is uniquely stabilized by disulfide bonds. In addition, during maturation an internal peptide of 35 residues is removed and three asparagine residues are glycosylated. In order to obtain insight into the effects of these structural features on CA function, wild-type C. reinhardtii alphaCA1 has been crystallized in space group P6(5), with unit-cell parameters a=b=134.3, c=120.2 A. The crystal diffracted to 1.88 A resolution and a preliminary solution of its crystal structure has been obtained by the MAD method.
authors.affiliation: College of Science and Engineering, Iwaki-Meisei University, Chuodai-iino, Iwaki, Fukushima 970-8551, Japan.
authors.forename: Kaoru
authors.initials: K
authors.lastname: Suzuki
authors.forename: Satoru
authors.initials: S
authors.lastname: Shimizu
authors.forename: Ella Czarina Magat
authors.initials: EC
authors.lastname: Juan
authors.forename: Takahiro
authors.initials: T
authors.lastname: Miyamoto
authors.forename: Zhang
authors.initials: Z
authors.lastname: Fang
authors.forename: Md Mominul
authors.initials: MM
authors.lastname: Hoque
authors.forename: Yoshiteru
authors.initials: Y
authors.lastname: Sato
authors.forename: Masaru
authors.initials: M
authors.lastname: Tsunoda
authors.forename: Takeshi
authors.initials: T
authors.lastname: Sekiguchi
authors.forename: Akio
authors.initials: A
authors.lastname: Takénaka
authors.forename: Shi Yuan
authors.initials: SY
authors.lastname: Yang
mesh_terms: D002256:Carbonic Anhydrases / Q000737:chemistry*; D016825:Chlamydomonas reinhardtii / Q000201:enzymology*; D018360:Crystallography, X-Ray
chemical_list: D002256:Carbonic Anhydrases
references.citation: Eur J Biochem. 1993 May 15;214(1):9-16
references.pmid: 8508810
references.citation: Proc Natl Acad Sci U S A. 1975 Jan;72(1):51-5
references.pmid: 804171
references.citation: J Bacteriol. 2004 Feb;186(3):623-30
references.pmid: 14729686
references.citation: Proc Natl Acad Sci U S A. 2001 Aug 14;98(17):9545-50
references.pmid: 11493685
references.citation: Methods Enzymol. 1997;276:307-26
references.pmid: 27754618
references.citation: Planta. 2001 Feb;212(3):454-9
references.pmid: 11289611
references.citation: J Physiol. 1933 Dec 5;80(2):113-42
references.pmid: 16994489
references.citation: Mol Phylogenet Evol. 1996 Feb;5(1):50-77
references.pmid: 8673298
references.citation: Proc Natl Acad Sci U S A. 1990 Dec;87(24):9779-83
references.pmid: 2124702
references.citation: EMBO J. 1998 Aug 10;17(5):1208-16
references.pmid: 9482718
references.citation: J Biol Chem. 2000 Feb 25;275(8):5521-6
references.pmid: 10681531
references.citation: Microbiology (Reading). 2000 Nov;146 ( Pt 11):2957-2966
references.pmid: 11065374
references.citation: Annu Rev Genet. 2008;42:619-45
references.pmid: 18983264
references.citation: EMBO J. 2002 Apr 15;21(8):1930-8
references.pmid: 11953312
references.citation: J Biol Chem. 2004 Feb 20;279(8):7223-8
references.pmid: 14660577
references.citation: EMBO J. 1996 May 15;15(10):2323-30
references.pmid: 8665839
references.citation: J Biol Chem. 2001 Mar 30;276(13):10299-305
references.pmid: 11096105
references.citation: Proc Natl Acad Sci U S A. 2009 Sep 22;106(38):16233-8
references.pmid: 19805286
references.citation: Proc Natl Acad Sci U S A. 1984 Oct;81(19):6049-53
references.pmid: 16593518
references.citation: Methods Mol Biol. 2007;364:215-30
references.pmid: 17172768
references.citation: J Biol Chem. 2001 Dec 28;276(52):48615-8
references.pmid: 11696553
references.citation: J Mol Biol. 1968 Apr 28;33(2):491-7
references.pmid: 5700707
references.citation: EMBO J. 2000 Apr 03;19(7):1407-18
references.pmid: 10747009
references.citation: Eur J Biochem. 1990 Sep 11;192(2):557-62
references.pmid: 2120056
references.citation: Proc Natl Acad Sci U S A. 1990 Jun;87(11):4383-7
references.pmid: 2112252
references.citation: J Bacteriol. 1999 Oct;181(20):6247-53
references.pmid: 10515911
references.citation: Nat Struct Biol. 1999 May;6(5):458-63
references.pmid: 10331874