title: Bovine tuberculosis at the wildlife-livestock-human interface in Hamer Woreda, South Omo, Southern Ethiopia.
abstract: Bovine tuberculosis (BTB) is endemic in cattle in the Ethiopian Highlands but no studies have been done so far in pastoralists in South Omo. This study assessed the prevalence of bovine tuberculosis (BTB) at an intensive interface of livestock, wildlife and pastoralists in Hamer Woreda (South Omo), Ethiopia. A cross-sectional survey including a comparative intradermal skin testing (CIDT) was conducted in 499 zebu cattle and 186 goats in 12 settlements. Sputum samples from 26 symptomatic livestock owners were cultured for TB. Fifty-one wildlife samples from 13 different species were also collected in the same area and tested with serological (lateral flow assay) and bacteriological (culture of lymph nodes) techniques. Individual BTB prevalence in cattle was 0.8% (CI: 0.3%-2%) with the >4 mm cut-off and 3.4% (CI: 2.1%-5.4%) with the >2 mm cut-off. Herd prevalence was 33.3% and 83% when using the >4 and the >2 mm cut-off respectively. There was no correlation between age, sex, body condition and positive reactors upon univariate analysis. None of the goats were reactors for BTB. Acid fast bacilli (AFB) were detected in 50% of the wildlife cultures, 79.2% of which were identified as Mycobacterium terrae complex. No M. bovis was detected. Twenty-seven percent of tested wildlife were sero-positive. Four sputum cultures (15.4%) yielded AFB positive colonies among which one was M. tuberculosis and 3 non-tuberculous mycobacteria (NTM). The prevalence of M. avium-complex (MAC) was 4.2% in wildlife, 2.5% in cattle and 0.5% in goats. In conclusion, individual BTB prevalence was low, but herd prevalence high in cattle and BTB was not detected in goats, wildlife and humans despite an intensive contact interface. On the contrary, NTMs were highly prevalent and some Mycobacterium spp were more prevalent in specific species. The role of NTMs in livestock and co-infection with BTB need further research.
authors.affiliation: Swiss Tropical and Public Health Institute, Basel, Switzerland. rea.tschopp@unibas.ch
authors.forename: Rea
authors.initials: R
authors.lastname: Tschopp
authors.forename: Abraham
authors.initials: A
authors.lastname: Aseffa
authors.forename: Esther
authors.initials: E
authors.lastname: Schelling
authors.forename: Stefan
authors.initials: S
authors.lastname: Berg
authors.forename: Elena
authors.initials: E
authors.lastname: Hailu
authors.forename: Endalamaw
authors.initials: E
authors.lastname: Gadisa
authors.forename: Meseret
authors.initials: M
authors.lastname: Habtamu
authors.forename: Kifle
authors.initials: K
authors.lastname: Argaw
authors.forename: Jakob
authors.initials: J
authors.lastname: Zinsstag
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D000835:Animals, Wild / Q000382:microbiology*; D002417:Cattle; D002648:Child; D002675:Child, Preschool; D005002:Ethiopia / Q000453:epidemiology; D005260:Female; D006041:Goats / Q000382:microbiology; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008875:Middle Aged; D015269:Mycobacterium avium Complex / Q000502:physiology; D014380:Tuberculosis, Bovine / Q000453:epidemiology*; D055815:Young Adult
references.citation: Prev Vet Med. 2009 May 1;89(1-2):81-9
references.pmid: 19269049
references.citation: J Bacteriol. 2005 Sep;187(18):6386-95
references.pmid: 16159772
references.citation: J Clin Microbiol. 1997 Apr;35(4):907-14
references.pmid: 9157152
references.citation: Trop Anim Health Prod. 2009 Apr;41(4):617-22
references.pmid: 18821086
references.citation: Epidemiol Infect. 2007 May;135(4):529-40
references.pmid: 16959052
references.citation: Am J Clin Pathol. 2002 Nov;118(5):796-801
references.pmid: 12428802
references.citation: J Hyg (Lond). 1977 Aug;79(1):155-60
references.pmid: 328766
references.citation: Vet Microbiol. 1998 Aug 15;62(4):281-90
references.pmid: 9791874
references.citation: Clin Vaccine Immunol. 2008 Aug;15(8):1272-6
references.pmid: 18495847
references.citation: J Wildl Dis. 2010 Jul;46(3):753-62
references.pmid: 20688681
references.citation: Trop Anim Health Prod. 2009 Feb;41(2):273-7
references.pmid: 18607767
references.citation: Mol Microbiol. 1999 May;32(3):643-55
references.pmid: 10320585
references.citation: PLoS One. 2009;4(4):e5068
references.pmid: 19352493
references.citation: BMC Vet Res. 2007 Sep 27;3:23
references.pmid: 17900356
references.citation: Vet Microbiol. 2008 Dec 10;132(3-4):283-92
references.pmid: 18602770
references.citation: J Clin Microbiol. 1995 Nov;33(11):2953-6
references.pmid: 8576352
references.citation: Prev Vet Med. 2005 Mar 15;67(4):237-66
references.pmid: 15748755
references.citation: Int J Tuberc Lung Dis. 2004 Aug;8(8):924-37
references.pmid: 15305473
references.citation: Tuberculosis (Edinb). 2001;81(3):191-202
references.pmid: 11466031
references.citation: Aust Vet J. 1998 Jan;76(1):50-3
references.pmid: 9578768
references.citation: Trop Anim Health Prod. 2009 Jun;41(5):755-65
references.pmid: 19058024
references.citation: Vet Microbiol. 2008 Apr 1;128(1-2):72-80
references.pmid: 17954015
references.citation: J Wildl Dis. 2005 Apr;41(2):446-53
references.pmid: 16107683
references.citation: PCR Methods Appl. 1992 May;1(4):269-73
references.pmid: 1282431
references.citation: Vet Rec. 2010 Aug 14;167(7):250-6
references.pmid: 20710033
references.citation: Prev Vet Med. 2009 Jun 1;89(3-4):205-11
references.pmid: 19339066
references.citation: Acta Trop. 2010 Jan;113(1):88-91
references.pmid: 19732736
references.citation: Clin Vaccine Immunol. 2007 Oct;14(10):1356-61
references.pmid: 17761523