title: Studies on absorption and metabolism of palatinose (isomaltulose) in rats.
abstract: We evaluated the absorption and metabolism of palatinose in rats by the carbohydrate load test and the 13C- and H2-breath tests. We compared the results of these tests with those of sucrose, since sucrose is an isomer of palatinose and generally known to be degraded and absorbed from the small intestine. In the carbohydrate load test, blood glucose and plasma insulin levels after oral administration of palatinose rose more gradually and reached a maximum that was lower than that after sucrose administration. In the 13C-breath test, rats were orally administrated [1-13C]sucrose or [1-13C]palatinose and housed in a chamber. The expired air in the chamber was collected, and the level of 13CO2 in the expired air was measured at appropriate intervals for 360 min. The value of time taken to reach the maximum concentration for expired 13CO2 from [1-13Cglucose] ([1-13Cglc]) and [1-13Cfructose] ([1-13Cfru]) palatinose was significantly longer than that from [1-13Cglc] and [1-13Cfru]sucrose, respectively. The value of area under the curve (AUC) for [1-13Cglc]palatinose was larger than that for [1-13Cglc]sucrose, but AUC for [1-13Cfru] showed no difference between palatinose and sucrose. In the H2-breath test, the concentration of H2 in the expired air was measured for 420 min. H2 was hardly detected with both palatinose and sucrose and no significant difference was observed between the two groups. These results suggest that palatinose is utilised in vivo at a rate equal to that of sucrose.
authors.affiliation: Division of Research and Development, Food Science Institute, Meiji Dairies Corporation, 540 Naruda, Odawara, Kanagawa 250 0862, Japan. hidekazu_tonouchi@meiji-milk.com
authors.forename: Hidekazu
authors.initials: H
authors.lastname: Tonouchi
authors.forename: Taketo
authors.initials: T
authors.lastname: Yamaji
authors.forename: Masayuki
authors.initials: M
authors.lastname: Uchida
authors.forename: Megumi
authors.initials: M
authors.lastname: Koganei
authors.forename: Akina
authors.initials: A
authors.lastname: Sasayama
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Kaneko
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Urita
authors.forename: Masahiro
authors.initials: M
authors.lastname: Okuno
authors.forename: Kouji
authors.initials: K
authors.lastname: Suzuki
authors.forename: Jun
authors.initials: J
authors.lastname: Kashimura
authors.forename: Hajime
authors.initials: H
authors.lastname: Sasaki
mesh_terms: D000284:Administration, Oral; D000818:Animals; D019540:Area Under Curve; D001786:Blood Glucose / Q000378:metabolism*; D001944:Breath Tests / Q000379:methods; D002245:Carbon Dioxide / Q000378:metabolism; D002247:Carbon Isotopes; D019422:Dietary Sucrose / Q000378:metabolism / Q000493:pharmacokinetics*; D006859:Hydrogen / Q000378:metabolism; D007328:Insulin / Q000097:blood*; D007408:Intestinal Absorption; D007421:Intestine, Small / Q000378:metabolism*; D007534:Isomaltose / Q000031:analogs & derivatives* / Q000378:metabolism / Q000493:pharmacokinetics; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013395:Sucrose / Q000378:metabolism / Q000493:pharmacokinetics
chemical_list: D001786:Blood Glucose; D002247:Carbon Isotopes; D019422:Dietary Sucrose; D007328:Insulin; D002245:Carbon Dioxide; D013395:Sucrose; D007534:Isomaltose; D006859:Hydrogen; C008189:isomaltulose