Biological active metabolite cyclo (L-Trp-L-Phe) produced by South China Sea sponge Holoxea sp. associated fungus Aspergillus versicolor strain TS08.
Sponge-associated fungi represent the single most prolific source of novel natural products from marine fungi. Cyclo (L-Trp-L-Phe) exhibits biological functions such as plant growth regulation, moderate cytotoxicity and thus has the application potential in pharmaceutical and agricultural biotechnologies. In this study, a fungal strain TS08 was isolated from sponge Holoxea sp. in the South China Sea and identified as A. versicolor according to its 18S rRNA gene and morphological, physiological, and biochemical characteristics. Meanwhile, cyclo (L-Trp-L-Phe) was found to be produced by A. versicolor strain TS08 mainly in the exponential growth phase. The highest yield of cyclo (L-Trp-L-Phe), 13.24 mg/g (per crude extract of EtOAc), 2.51% of cell dry weigh, was obtained on the tenth day of the fungal cultivation. It was the first time to find the biological active cyclo (L-Trp-L-Phe) in sponge-associated microorganism.
Chu
Dan
D
Laboratory of Marine Biotechnology, School of Life Sciences and Biotechnology and Key Laboratory of Microbial Metabolism, Ministry of Education, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240, People's Republic of China.
Peng
Chongsheng
C
Ding
Bo
B
Liu
Fang
F
Zhang
Fengli
F
Lin
Houwen
H
Li
Zhiyong
Z
D000818:Animals; D001230:Aspergillus / Q000235:genetics / Q000378:metabolism*; D004151:Dipeptides / Q000096:biosynthesis*; D010456:Peptides, Cyclic / Q000096:biosynthesis*; D011161:Porifera / Q000382:microbiology*; D012337:RNA, Ribosomal, 18S / Q000235:genetics
D004151:Dipeptides; D010456:Peptides, Cyclic; D012337:RNA, Ribosomal, 18S