Protein profiling of Guillain-Barrè syndrome cerebrospinal fluid by two-dimensional electrophoresis and mass spectrometry.
Protein profiling of cerebrospinal fluid in Guillain-Barrè syndrome (GBS), an acute and immune-mediated disease affecting the peripheral nervous system, was performed by two-dimensional electrophoresis. Significant modulated spots in GBS patients vs. control groups (a group of multiple sclerosis patients and one of healthy donors) underwent MALDI-TOF/TOF investigation. Inflammation-related proteins, such as vitamin D-binding protein, beta-2 glycoprotein I (ApoH), and a complement component C3 isoform were up-regulated in GBS, whereas transthyretin (the monomer and the dimer forms), apolipoprotein E, albumin and five of its fragments were down-regulated. Then, we used an isoelectric-focusing-dinitrophenylhydrazine-based technique to analyse the extent of carbonylation and, as a result, of oxidative damage of GBS CSF proteome. We observed a major sensitivity to carbonylation for albumin and alpha-glycoprotein in inflammation and a selective increase of reactivity for a glycosylated Fab from an IgM globulin in GBS CSF. Our results add new proteins to candidate CSF features of GBS, and suggest that oxidative stress could contribute to the immunopathological mechanisms in this syndrome.
D'Aguanno
Simona
S
Centro Europeo Ricerca sul Cervello, IRCCS-Fondazione Santa Lucia, Rome, Italy.
Franciotta
Diego
D
Lupisella
Santina
S
Barassi
Alessandra
A
Pieragostino
Damiana
D
Lugaresi
Alessandra
A
Centonze
Diego
D
D'Eril
Gianlodovico Melzi
GM
Bernardini
Sergio
S
Federici
Giorgio
G
Urbani
Andrea
A
D000293:Adolescent; D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000134:cerebrospinal fluid; D002556:Cerebrospinal Fluid Proteins / Q000096:biosynthesis*; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D020869:Gene Expression Profiling; D020275:Guillain-Barre Syndrome / Q000134:cerebrospinal fluid* / Q000276:immunology; D006801:Humans; D007525:Isoelectric Focusing; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D050050:Protein Carbonylation; D020543:Proteome / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D015415:Biomarkers; D002556:Cerebrospinal Fluid Proteins; D020543:Proteome