title: Urinary matrix metalloproteinase-7 level is associated with the presence of metastasis in bladder cancer.
abstract: OBJECTIVE
  • To assess the presence of matrix metalloproteinase (MMP)-7 in urine samples of patients with bladder cancer and to investigate the correlation between MMP-7 urine concentration and clinicopathological variables.
  
  
  METHODS
  • The presence of MMP-7 in the urine of patients with bladder cancer was tested in 32 representative cases using immunoprecipitation followed by western blot analysis. • Urinary MMP-7 concentration levels were analyzed in 132 patients with bladder cancer and 96 controls using an enzyme-linked immunosorbent assay.
  
  
  RESULTS
  • MMP-7 levels did not differ significantly between patients with localized bladder cancer and controls (P= 0.174). On the other hand, we detected a fourfold, significantly elevated MMP-7 concentration in urine samples of patients with bladder cancer with regional or distant metastasis (P= 0.003). • Using a threshold value of 6.88 ng/ml, determined by receiver-operating characteristic curve analysis, a specificity of 82% and a sensitivity of 78% were observed. • Western blot analysis revealed that the 55-kDa tissue inhibitor of metalloproteinase 1 complexed MMP-7 is the dominant form of urinary matrilysin.
  
  
  CONCLUSIONS
  • MMP-7 is present in detectable amounts in the urine of patients with bladder cancer. Its concentrations are significantly elevated in patients with metastatic disease. • Determination of urinary matrilysin level could help to detect bladder cancer metastasis, and may therefore provide a more reliable prognosis and influence therapy decisions.
authors.affiliation: Department of Urology, University Hospital of Essen, Essen, Germany. sztibusz@gmail.com
authors.forename: Tibor
authors.initials: T
authors.lastname: Szarvas
authors.forename: Bernhard B
authors.initials: BB
authors.lastname: Singer
authors.forename: Markus
authors.initials: M
authors.lastname: Becker
authors.forename: Frank
authors.initials: F
authors.lastname: Vom Dorp
authors.forename: Tobias
authors.initials: T
authors.lastname: Jäger
authors.forename: Attila
authors.initials: A
authors.lastname: Szendroi
authors.forename: Péter
authors.initials: P
authors.lastname: Riesz
authors.forename: Imre
authors.initials: I
authors.lastname: Romics
authors.forename: Herbert
authors.initials: H
authors.lastname: Rübben
authors.forename: Süleyman
authors.initials: S
authors.lastname: Ergün
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000097:blood / Q000652:urine*; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D020783:Matrix Metalloproteinase 7 / Q000097:blood / Q000652:urine*; D008875:Middle Aged; D011379:Prognosis; D012680:Sensitivity and Specificity; D001749:Urinary Bladder Neoplasms / Q000201:enzymology* / Q000473:pathology* / Q000652:urine
chemical_list: D014408:Biomarkers, Tumor; D020783:Matrix Metalloproteinase 7