title: Hypermethylation of SOX2 Promoter in Endometrial Carcinogenesis.
abstract: This paper aimed at investigating the expression and methylation profiles of SOX2, a gene coding for the stem cell-related transcription factor SOX2, in endometrial carcinomas. By methylation-specific polymerase chain reaction (MS-PCR), the methylation status of SOX2 promoter region in 72 endometrial carcinomas and 12 normal endometrial samples was examined. Methylated allele was found in 37.5% (27/72) of endometrial carcinomas but only in 8.3% (1/12) of normal endometrial, significantly more frequent in cancers (P = .0472). SOX2 mRNA level was significantly reduced in endometrial carcinoma compared with nonneoplastic endometrium (P = .045). A significant correlation between SOX2 mRNA expression and hypermethylation of SOX2 was found (P = .024). Hypermethylation of SOX2 tended to be more frequently found in type II serous or clear cell adenocarcinoma. SOX2 methylation was also significantly correlated with shorter survival of patients (P = .046). In conclusion, epigenetic mechanisms may play a crucial role on the transcriptional regulation of SOX2 and loss of SOX2 expression may be related to endometrial carcinogenesis.
authors.affiliation: Department of Pathology, Queen Mary Hospital, The University of Hong Kong, Pokfulam Road, Hong Kong.
authors.forename: Oscar Gee-Wan
authors.initials: OG
authors.lastname: Wong
authors.forename: Zhen
authors.initials: Z
authors.lastname: Huo
authors.forename: Michelle Kwan-Yee
authors.initials: MK
authors.lastname: Siu
authors.forename: Huijuan
authors.initials: H
authors.lastname: Zhang
authors.forename: Lili
authors.initials: L
authors.lastname: Jiang
authors.forename: Ester Shuk-Ying
authors.initials: ES
authors.lastname: Wong
authors.forename: Annie Nga-Yin
authors.initials: AN
authors.lastname: Cheung
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