In Vitro Study of Nitric Oxide Metabolites Effects on Human Hydatid of Echinococcus granulosus.
Hydatidosis is characterized by the long-term coexistence of larva Echinococcus granulosus and its host without effective rejection. Previous studies demonstrated nitric oxide (NO) production (in vivo and in vitro) during hydatidosis. In this study, we investigated the direct in vitro effects of NO species: nitrite (NO(2) (-)), nitrate (NO(3) (-)) and peroxynitrite (ONOO(-)) on protoscolices (PSCs) viability and hydatid cyst layers integrity for 24 hours and 48 hours. Our results showed protoscolicidal activity of NO(2) (-) and ONOO(-) 24 hours and 3 hours after treatment with 320 muM and 80 muM respectively. Degenerative effects were observed on germinal and laminated layers. The comparison of the in vitro effects of NO species on the PSCs viability indicated that ONOO(-) is more cytotoxic than NO(2) (-). In contrast, NO(3) (-) has no effect. These results suggest possible involvement of NO(2) (-) and ONOO(-) in antihydatic action and point the efficacy of these metabolites as scolicidal agents.
Zeghir-Bouteldja
Razika
R
Laboratoire de Biologie Cellulaire et Moléculaire, FSB-USTHB, Université Bab-Ezzouar, Bp 32, 16111 Algiers, Algeria.
Amri
Manel
M
Aitaissa
Saliha
S
Bouaziz
Samia
S
Mezioug
Dalila
D
Touil-Boukoffa
Chafia
C
Methods Enzymol. 1996;269:296-302
8791658
Recent Pat Antiinfect Drug Discov. 2009 Jan;4(1):29-36
19149694
Am J Physiol. 1996 Nov;271(5 Pt 1):C1424-37
8944624
Cell Mol Biol (Noisy-le-grand). 2006 May 15;52(1):65-70
16914097
Pathol Biol (Paris). 1997 Feb;45(2):110-4
9247032
Parasite Immunol. 2001 Aug;23(8):411-7
11489164
Trans R Soc Trop Med Hyg. 2000 May-Jun;94(3):319-22
10975011
Biochimie. 1998 Aug-Sep;80(8-9):739-44
9865496
Parasitology. 1996 Mar;112 ( Pt 3):315-22
8728995
Clin Exp Dermatol. 1997 Sep;22(5):244-5
9536549
Res Immunol. 1995 Nov-Dec;146(9):689-92
8852612
Free Radic Biol Med. 2001 Jun 1;30(11):1234-42
11368921
Parasite Immunol. 1993 Nov;15(11):619-23
7877838
Arch Biochem Biophys. 1991 Aug 1;288(2):481-7
1654835
Int J Parasitol. 2000 Nov;30(12-13):1283-94
11113255
J Interferon Cytokine Res. 2007 Sep;27(9):781-7
17892399
Proc Natl Acad Sci U S A. 1990 Feb;87(4):1620-4
2154753
Biol Chem. 2000 Jul;381(7):623-7
10987371
Biochem Biophys Res Commun. 1995 Oct 24;215(3):1111-8
7488038
AJR Am J Roentgenol. 2005 Sep;185(3):727-34
16120926
J Immunol. 1990 Jan 1;144(1):278-83
2104889
Adv Parasitol. 1996;37:1-56
8881597
Curr Top Microbiol Immunol. 1995;196:57-73
7634825
Gastroenterol Clin North Am. 1996 Sep;25(3):655-89
8863045
Exp Parasitol. 2007 Jul;116(3):257-65
17336295
N Engl J Med. 1997 Sep 25;337(13):881-7
9302302
Eur Surg Res. 1996;28(6):466-70
8954324
Int Immunopharmacol. 2001 Aug;1(8):1457-67
11515811
Eur Cytokine Netw. 2009 Jun;20(2):63-8
19541591
Int J Mol Med. 2002 Feb;9(2):131-4
11786922
Arch Biochem Biophys. 2000 May 1;377(1):146-52
10775454