Dietary RRR-α-tocopherol succinate attenuates lipopolysaccharide-induced inflammatory cytokines secretion in broiler chicks.
The anti-inflammatory effects of two esters of a-tocopherol (α-TOH), all-rac-α-TOH acetate (DL-α-TOA) and RRR-α-TOH succinate (D-α-TOS), on broilers repeatedly challenged with lipopolysaccharide (LPS) were investigated. Three hundred and twenty 1-d-old broiler chicks were allotted into four treatment groups and fed on a control diet (30 mg/kg DL-α-TOA) or diets containing 10, 30, 50 mg/kg D-α-TOS. Half of the birds from each treatment group were challenged with 0·9% NaCl solution or LPS (250 μg/kg body weight) at 16, 18 and 20 d of age. The results indicated that the pretreatment of birds with 50 mg/kg D-α-TOS markedly reduced serum PGE₂ secretion and increased the concentrations of serum or hepatic α-TOH. When LPS-challenged birds were pretreated with 30 or 50 mg/kg D-α-TOS, the increases of plasma and splenic concentrations of interferon-γ, IL-1ß, IL-2, IL-6, IL-4 and IL-10 were dramatically attenuated. Also, a significant decrease of hepatic reactive oxygen species (ROS) and hepatic or splenic phosphokinase C (PKC) activities was found in birds pretreated with 30 or 50 mg/kg D-α-TOS. Furthermore, D-α-TOS inhibited the activation of NF-κB by preventing the degradation of inhibitory-κBα. In conclusion, D-α-TOS is able to prevent LPS-induced inflammation response in vivo. The beneficial effect may depend on suppressing the secretion of various plasma and splenic inflammatory mediators through inhibiting NF-κB activation and by blocking ROS signalling, in which PKC may play an assistant role.
Zhang
Xuhui
X
Nanjing Agricultural University, Xuanwu District, People’s Republic of China.
Zhong
Xiang
X
Zhou
Yanmin
Y
Wang
Gaiqin
G
Du
Haiming
H
Wang
Tian
T
D000821:Animal Feed / Q000032:analysis*; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D002645:Chickens / Q000254:growth & development / Q000502:physiology*; D016207:Cytokines / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D004032:Diet / Q000662:veterinary*; D019587:Dietary Supplements; D007249:Inflammation / Q000378:metabolism; D008070:Lipopolysaccharides / Q000633:toxicity; D008099:Liver / Q000378:metabolism; D011201:Poultry Diseases / Q000517:prevention & control; D011493:Protein Kinase C / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D013154:Spleen / Q000378:metabolism; D024505:Tocopherols / Q000737:chemistry* / Q000494:pharmacology*
D016207:Cytokines; D008070:Lipopolysaccharides; D017382:Reactive Oxygen Species; D011493:Protein Kinase C; D024505:Tocopherols