Negative regulation of neuronal cell differentiation by INHAT subunit SET/TAF-Iβ.
Epigenetic modification plays an important role in transcriptional regulation. As a subunit of the INHAT (inhibitor of histone acetyltransferases) complex, SET/TAF-Iβ evidences transcriptional repression activity. In this study, we demonstrate that SET/TAF-Iβ is abundantly expressed in neuronal tissues of Drosophila embryos. It is expressed at high levels prior to and in early stages of neuronal development, and gradually reduced as differentiation proceeds. SET/TAF-Iβ binds to the promoters of a subset of neuronal development markers and negatively regulates the transcription of these genes. The results of this study show that the knockdown of SET/TAF-Iβ by si-RNA induces neuronal cell differentiation, thus implicating SET/TAF-Iβ as a negative regulator of neuronal development.
Kim
Dong-Wook
DW
Department of Life Science, College of Natural Sciences, Chung-Ang University, Seoul 156-756, South Korea.
Kim
Kee-Beom
KB
Kim
Ji-Young
JY
Lee
Kyu-Sun
KS
Seo
Sang-Beom
SB
D000818:Animals; D045744:Cell Line, Tumor; D004268:DNA-Binding Proteins; D004331:Drosophila melanogaster / Q000166:cytology / Q000235:genetics / Q000254:growth & development; D018507:Gene Expression Regulation, Developmental; D055785:Gene Knockdown Techniques; D056488:Histone Chaperones / Q000378:metabolism*; D006801:Humans; D047468:Immunoprecipitation; D055495:Neurogenesis / Q000235:genetics*; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D034741:RNA, Small Interfering / Q000235:genetics; D014157:Transcription Factors / Q000378:metabolism*; D014158:Transcription, Genetic
D004268:DNA-Binding Proteins; D056488:Histone Chaperones; D034741:RNA, Small Interfering; C513473:SET protein, human; D014157:Transcription Factors