title: Morton lentil extract attenuated angiotensin II-induced cardiomyocyte hypertrophy via inhibition of intracellular reactive oxygen species levels in vitro.
abstract: The objective was to investigate whether a lentil (Morton) extract had any protective effect on cardiac hypertrophy, which is one of the most significant sequelae of cardiovascular diseases. High phenolic compounds (43.4 mg of GAE/g), including thirteen phenolic acid and two flavonoids, were detected in the acetone/water/acetic acid lentil extract. The extract showed strong antioxidant ability (105 μmol of TE/g). The effect of lentil extract on angiotensin (Ang) II-induced cardiac hypertrophy was examined. Results showed that pretreatment with lentil extract (25, 50, 100 μg/mL) significantly attenuated Ang II (0.1 μM)-induced hypertrophy by 18, 28, and 36% in rat cardiomycytes, respectively; lentil extract (12.5, 25, 50 μg/mL) attenuated Ang II (0.1 μM)-induced hypertrophy by 9, 17, and 25% in human cardiomycytes, respectively. Intracellular reactive oxygen species (ROS) levels were enhanced by Ang II treatment, and this stimulatory action was significantly attenuated (33% inhibition) by lentil extract (100 μg/mL) in rat cardiomyocytes and attenuated by 22% by 50 μg/mL lentil extract in human cardiomyocytes. In conclusion, Morton lentil extracts attenuated Ang II-induced rat and human cardiomyocytes hypertrophy via decreasing intracellular ROS levels.
authors.affiliation: Department of Food and Cereal Sciences, North Dakota State University, 1320 Albrecht Boulevard, Fargo, North Dakota 58105, USA.
authors.forename: Fan-Rong
authors.initials: FR
authors.lastname: Yao
authors.forename: Cheng-Wen
authors.initials: CW
authors.lastname: Sun
authors.forename: Sam K C
authors.initials: SK
authors.lastname: Chang
mesh_terms: D000804:Angiotensin II / Q000494:pharmacology*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D002312:Cardiomyopathy, Hypertrophic / Q000139:chemically induced / Q000517:prevention & control*; D002478:Cells, Cultured; D006801:Humans; D027806:Lens Plant / Q000737:chemistry*; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000473:pathology; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors*
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D000804:Angiotensin II