Optimization of enantioselective synthesis of methyl (R)-2-chloromandelate by whole cells of Saccharomyces cerevisiae.
Methyl (R)-2-chloromandelate, a key intermediate in the synthesis of clopidogrel, was obtained by the reduction of methyl-2-chlorobenzoylformate using whole cells of Saccharomyces cerevisiae. A 100% conversion and 96.1% of enantiomeric excess (ee) value was obtained when 17 methyl-2-chlorobenzoylformate/l was reacted with 8 g S. cerevisiae/l and 83 g glucose/l at pH 7.
Jeong
Min
M
School of Chemical Engineering and Bioengineering, University of Ulsan, 29 Mugeo-dong Nam-gu, Ulsan, 680-749, Republic of Korea.
Lee
Yoon Mi
YM
Hong
Soon Ho
SH
Park
Sung Young
SY
Yoo
Ik-keun
IK
Han
Mee Jung
MJ
D001711:Biotransformation; D005947:Glucose / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008333:Mandelic Acids / Q000737:chemistry / Q000378:metabolism*; D010648:Phenylacetates / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D013237:Stereoisomerism
D008333:Mandelic Acids; D010648:Phenylacetates; C522563:methyl 2-chlorobenozylformate; C522562:methyl 2-chloromandelate; D005947:Glucose