Crystallization and calcium/sulfur SAD phasing of the human EF-hand protein S100A2.
Human S100A2 is an EF-hand protein and acts as a major tumour suppressor, binding and activating p53 in a Ca2+-dependent manner. Ca2+-bound S100A2 was crystallized and its structure was determined based on the anomalous scattering provided by six S atoms from methionine residues and four calcium ions present in the asymmetric unit. Although the diffraction data were recorded at a wavelength of 0.90 A, which is usually not assumed to be suitable for calcium/sulfur SAD, the anomalous signal was satisfactory. A nine-atom substructure was determined at 1.8 A resolution using SHELXD, and SHELXE was used for density modification and phase extension to 1.3 A resolution. The electron-density map obtained was well interpretable and could be used for automated model building by ARP/wARP.
Koch
Michael
M
Department of Biology, University of Konstanz, Postfach M665, Universitätsstrasse 10, 78457 Konstanz, Germany.
Diez
Joachim
J
Wagner
Armin
A
Fritz
Günter
G
D002118:Calcium / Q000737:chemistry*; D002630:Chemotactic Factors / Q000737:chemistry*; D018360:Crystallography, X-Ray; D020832:EF Hand Motifs; D006801:Humans; D008958:Models, Molecular; D017434:Protein Structure, Tertiary; D009418:S100 Proteins / Q000737:chemistry*; D013455:Sulfur / Q000737:chemistry*
D002630:Chemotactic Factors; D009418:S100 Proteins; C098997:S100A2 protein, human; D013455:Sulfur; D002118:Calcium
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