A sperm viability test using SYBR-14/propidium iodide flow cytometry as a tool for rapid screening of primary ciliary dyskinesia patients and for choosing sperm sources for intracytoplasmic sperm injection.
Spermatozoa viability tests based on dual-color flow cytometry after staining with Sybr-14/propidium iodide were performed on 44 men with complete asthenospermia for primary ciliary dyskinesia (PCD) screening, and seven were identified with PCD by electron microscopy of ultrastructural ciliary defects. Six PCD patients underwent eight intracytoplasmic sperm injection therapy cycles using ejaculated sperm or testicular sperm, obtaining a mean fertilization rate of 46.6%, with three healthy babies born and one in utero at the time of writing.
Niu
Zhi-hong
ZH
Department of Gynecology and Obstetrics, Medical School of Fudan University, and Reproductive Medical Center, Ruijin Hospital Affiliated to Medical School of Shanghai Jiaotong University, Shanghai, People's Republic of China.
Huang
Xue-feng
XF
Jia
Xiao-feng
XF
Zheng
Jufen
J
Yuan
Yao
Y
Shi
Ting-yan
TY
Diao
Hua
H
Yu
He-guo
HG
Sun
Fei
F
Zhang
Hui-qing
HQ
Shi
Hui-juan
HJ
Feng
Yun
Y
D000328:Adult; D053627:Asthenozoospermia / Q000175:diagnosis* / Q000209:etiology / Q000628:therapy*; D002470:Cell Survival; D002925:Ciliary Motility Disorders / Q000150:complications / Q000175:diagnosis*; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D008297:Male; D008403:Mass Screening / Q000379:methods; D009930:Organic Chemicals; D011419:Propidium; D020554:Sperm Injections, Intracytoplasmic; D013094:Spermatozoa / Q000166:cytology*
D009930:Organic Chemicals; C093207:SYBR-14; D011419:Propidium