title: Molecular characterization of Ramie mosaic virus isolates detected in Jiangsu and Zhejiang provinces, China.
abstract: Virus isolates were obtained from three ramie samples (Boehmeria nivea L.) showing yellow mosaic symptoms collected in Jiangsu and Zhejiang provinces, China. Comparison of partial DNA-A fragments amplified with begomovirus universal primers PA/PB revealed that these viral isolates shared a high sequence identity. The complete DNA-A sequences of two isolates J4 and Z1 were determined to be 2736 and 2737 nts, respectively, sharing 94.7% nucleotide sequence identity with each other. Also, the DNA-B components were identified for J4 and Z1 isolates and comprised 2717 and 2719 nts, respectively, sharing 88.6% nucleotide sequence identity with each other. Furthermore, sequence alignment and phylogenetic analysis showed that J4 and Z1 isolates had the highest sequence identities (93.6-94.7%) with isolates of Ramie mosaic virus (RamMV) for DNA-A. These molecular data suggested that J4 and Z1 may be two different isolates of RamMV. This is the first report about the occurrence of a bipartite begomovirus in these regions of China.
authors.affiliation: Institute of Biotechnology, Zhejiang University, Hangzhou, P.R. China.
authors.forename: J
authors.initials: J
authors.lastname: Li
authors.forename: X Y
authors.initials: XY
authors.lastname: Zhang
authors.forename: Y J
authors.initials: YJ
authors.lastname: Qian
mesh_terms: D053839:Begomovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D032402:Boehmeria / Q000821:virology*; D002681:China; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*