Short-term storage and transport at cold temperatures of 2-cell mouse embryos produced by cryopreserved sperm.
At refrigerated temperatures, mouse embryos can maintain developmental ability for short periods. Previously, we succeeded in transporting vitrified and warmed 2-cell mouse embryos while maintaining developmental ability at refrigerated temperatures for 50 h. Transport of nonfrozen embryos is an easier and more useful means of exchanging genetically engineered mice between laboratories than is transport of cryopreserved embryos. Here we examined the developmental ability of transported 2-cell embryos that were produced through in vitro fertilization using cryopreserved sperm. Results show that 2-cell embryos produced by cryopreserved sperm can develop into blastocysts after cold storage for 24, 48, and 72 h. Transported 2-cell embryos produced by cryopreserved sperm yielded a favorable number of pups in all of the receiving laboratories after transport lasting 48 to 52 h. In summary, cold storage and transport of 2-cell embryos derived from cryopreserved sperm at refrigerated temperatures provides a novel means of transporting genetically engineered mice as an alternative to the transport of cryopreserved embryos and sperm.
Takeo
Toru
T
Division of Reproductive Engineering, Center for Animal Resources and Development (CARD), Kumamoto University, Kumamoto, Japan.
Kondo
Tomoko
T
Haruguchi
Yukie
Y
Fukumoto
Kiyoko
K
Nakagawa
Yoshiko
Y
Takeshita
Yumi
Y
Nakamuta
Yuko
Y
Tsuchiyama
Shuuji
S
Shimizu
Norihiko
N
Hasegawa
Takanori
T
Goto
Motohito
M
Miyachi
Hitoshi
H
Anzai
Masayuki
M
Fujikawa
Rie
R
Nomaru
Koji
K
Kaneko
Takehito
T
Itagaki
Yoshiaki
Y
Nakagata
Naomi
N
D000818:Animals; D003080:Cold Temperature; D015925:Cryopreservation; D003470:Culture Media; D046149:Embryo Culture Techniques; D004624:Embryo Transfer; D047108:Embryonic Development; D005260:Female; D005307:Fertilization in Vitro; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR; D013094:Spermatozoa; D014186:Transportation
D003470:Culture Media
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