title: Electrical field stimulation promotes anastomotic healing in poorly perfused rat colon.
abstract: BACKGROUND
  Hypoperfusion of the bowel is a risk factor for anastomotic failure. Electrical field stimulation has been shown to improve repair in ischemic tissue, but its influence in hypoperfused colon has not been investigated. The hypothesis of this experimental animal study was that electrical field stimulation improves anastomotic healing in ischemic bowel.
  
  
  METHODS
  Thirty rats were divided evenly into three groups: control, ischemia/placebo, and ischemia/test group. Ischemia was induced by ligation of the arterial supply to the proximal colon. The watershed area was identified and transected. Field stimulation was achieved by application of negatively charged diethylaminoethyl Sephadex beads in methylcellulose gel to the colonic epithelium prior to anastomosis. The placebo group had methylcellulose gel only applied and control animals had anastomosis only. Anastomotic strength was measured using anastomotic bursting pressure and hydroxyproline content. Systemic effect was investigated via interleukin-6 and vascular endothelial growth factor assay.
  
  
  RESULTS
  The ischemia/electrical field stimulation (EFS) group had significantly increased bursting pressure and hydroxyproline content in comparison with the placebo group (P < 0.001). Serum cytokine levels were unaffected.
  
  
  CONCLUSIONS
  Negatively charged EFS improves anastomotic healing in hypoperfused colon without induction of systemic cytokines and has potential as a local treatment in high-risk bowel anastomosis.
authors.affiliation: Institute for Clinical Outcomes, Research and Education, St Vincent's University Hospital, Dublin 00004, Ireland. kennellyrory@yahoo.ie
authors.forename: Rory
authors.initials: R
authors.lastname: Kennelly
authors.forename: John B
authors.initials: JB
authors.lastname: Conneely
authors.forename: David J
authors.initials: DJ
authors.lastname: Bouchier-Hayes
authors.forename: Desmond C
authors.initials: DC
authors.lastname: Winter
mesh_terms: D000714:Anastomosis, Surgical; D000792:Angiography; D000818:Animals; D003106:Colon / Q000098:blood supply* / Q000000981:diagnostic imaging / Q000601:surgery*; D004599:Electric Stimulation Therapy; D006909:Hydroxyproline / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008297:Male; D010477:Perfusion; D011312:Pressure; D051381:Rats; D017207:Rats, Sprague-Dawley; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism; D014945:Wound Healing
chemical_list: D015850:Interleukin-6; D042461:Vascular Endothelial Growth Factor A; D006909:Hydroxyproline
references.citation: Am J Physiol Gastrointest Liver Physiol. 2004 Mar;286(3):G452-7
references.pmid: 14563675
references.citation: Colorectal Dis. 2003 Sep;5(5):478-82
references.pmid: 12925084
references.citation: J Clin Immunol. 1996 May;16(3):159-64
references.pmid: 8734359
references.citation: Ann Surg. 2007 Feb;245(2):254-8
references.pmid: 17245179
references.citation: Nature. 2006 Jul 27;442(7101):457-60
references.pmid: 16871217
references.citation: FASEB J. 2004 Aug;18(11):1231-3
references.pmid: 15208265
references.citation: J Invest Dermatol. 1964 Nov;43:441-2
references.pmid: 14216525
references.citation: Ann Surg. 2004 Aug;240(2):255-9
references.pmid: 15273549
references.citation: Eur J Surg Oncol. 2009 Apr;35(4):444-6
references.pmid: 18417314
references.citation: Clin Biochem. 1996 Jun;29(3):225-9
references.pmid: 8740508
references.citation: ANZ J Surg. 2006 Jul;76(7):579-85
references.pmid: 16813622
references.citation: Electromagn Biol Med. 2006;25(4):307-23
references.pmid: 17178589
references.citation: Chir Ital. 2007 Sep-Oct;59(5):599-609
references.pmid: 18019632
references.citation: J Altern Complement Med. 2007 Jun;13(5):485-90
references.pmid: 17604552
references.citation: Br J Surg. 2005 Sep;92(9):1150-4
references.pmid: 16035134
references.citation: Connect Tissue Res. 2007;48(4):188-97
references.pmid: 17653975
references.citation: Arch Surg. 2002 Jul;137(7):813-7
references.pmid: 12093338
references.citation: J Surg Oncol. 2008 Feb 1;97(2):180-5
references.pmid: 18095268
references.citation: Tissue Eng. 2004 Sep-Oct;10(9-10):1558-65
references.pmid: 15588415
references.citation: Hepatogastroenterology. 1992 Aug;39(4):304-8
references.pmid: 1427571
references.citation: Bioelectromagnetics. 1998;19(2):68-74
references.pmid: 9492161
references.citation: Br J Dermatol. 1983 Nov;109(5):515-22
references.pmid: 6639877
references.citation: J Cell Sci. 2004 Jan 26;117(Pt 3):397-405
references.pmid: 14679307
references.citation: Br J Surg. 2004 Dec;91(12):1608-12
references.pmid: 15549779
references.citation: J Bone Miner Res. 1988 Apr;3(2):165-71
references.pmid: 2463737
references.citation: J Gastrointest Surg. 2007 Jan;11(1):8-15
references.pmid: 17390180
references.citation: Wound Repair Regen. 1998 Nov-Dec;6(6):531-42
references.pmid: 9893173
references.citation: Bioelectromagnetics. 2005 Apr;26(3):207-14
references.pmid: 15768429
references.citation: Prog Clin Biol Res. 1986;210:231-8
references.pmid: 3960912