title: Pyruvate carboxylase is expressed in human skeletal muscle.
abstract: Pyruvate carboxylase (PC) is a mitochondrial enzyme that catalyses the carboxylation of pyruvate to oxaloacetate thereby allowing supplementation of citric acid cycle intermediates. The presence of PC in skeletal muscle is controversial. We report here, that PC protein is easily detectable by streptavidin blot and describe the presence of considerable amounts of PC in cultured human myotubes and in human muscle tissue.
authors.affiliation: Department of Endocrinology, Odense University Hospital, Denmark.
authors.forename: Ariane D
authors.initials: AD
authors.lastname: Minet
authors.forename: Michael
authors.initials: M
authors.lastname: Gaster
mesh_terms: D006801:Humans; D008931:Mitochondria, Muscle / Q000201:enzymology; D018485:Muscle Fibers, Skeletal / Q000201:enzymology; D018482:Muscle, Skeletal / Q000201:enzymology*; D011766:Pyruvate Carboxylase / Q000032:analysis / Q000096:biosynthesis*; D019809:Streptavidin / Q000737:chemistry
chemical_list: D019809:Streptavidin; D011766:Pyruvate Carboxylase