title: Galectin-1 and HIV-1 Infection.
abstract: Initial binding of human immunodeficiency virus-1 (HIV-1) to its susceptible CD4(+) cells is the limiting step for the establishment of infection as the avidity of viral envelope gp120 for CD4 is not high and the number of viral envelope spikes on the surface is found to be low compared to highly infectious viruses. Several host factors, such as C-type lectins, are listed as being able to enforce or facilitate the crucial interaction of HIV-1 to the susceptible cell. Recent works suggest that a host soluble beta-galactoside-binding lectin, galectin-1, also facilitates both virion binding and the infection of target cells in a manner dependent on lactose but not mannose, suggesting that this soluble galectin can be considered as a host factor that influences HIV-1 pathogenesis. In this chapter, we describe methods used to investigate the potential role of the galectin family in HIV-1-mediated disease progression.
authors.affiliation: Glycobiology and Bioimaging Laboratory, Research Centre for Infectious Diseases, Faculty of Medicine, Laval University, Quebec, Canada.
authors.forename: Christian
authors.initials: C
authors.lastname: St-Pierre
authors.forename: Michel
authors.initials: M
authors.lastname: Ouellet
authors.forename: Michel J
authors.initials: MJ
authors.lastname: Tremblay
authors.forename: Sachiko
authors.initials: S
authors.lastname: Sato
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D002460:Cell Line; D002623:Chemistry Techniques, Analytical / Q000379:methods; D037483:Galectin 1 / Q000302:isolation & purification / Q000378:metabolism / Q000502:physiology*; D015658:HIV Infections / Q000209:etiology* / Q000378:metabolism / Q000821:virology; D015497:HIV-1 / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D012449:Safety; D014773:Virology / Q000379:methods; D053585:Virus Attachment
chemical_list: D037483:Galectin 1