title: Diagnosis of prosthetic joint infection by beadmill processing of a periprosthetic specimen.
abstract: We report a microbiological process for the documentation of prosthetic joint infection (PJI). Intraoperative periprosthetic tissue samples from 92 consecutive patients undergoing revision surgery for PJI were submitted to mechanized beadmill processing: specimens were aseptically collected in polypropylene vials, filled with sterile water and glass beads and submitted to mechanized agitation with a beadmill. The documentation rate of PJI following culture on solid and liquid media was 83.7% and the contamination rate 8.7%. Final documentation was obtained after overnight culture for 51.9% of cases and with 7 days of broth culture for all documented cases.
authors.affiliation: Université de Versailles Saint-Quentin-en-Yvelines, EA3647, Garches, France.
authors.forename: A-L
authors.initials: AL
authors.lastname: Roux
authors.forename: V
authors.initials: V
authors.lastname: Sivadon-Tardy
authors.forename: T
authors.initials: T
authors.lastname: Bauer
authors.forename: A
authors.initials: A
authors.lastname: Lortat-Jacob
authors.forename: J-L
authors.initials: JL
authors.lastname: Herrmann
authors.forename: J-L
authors.initials: JL
authors.lastname: Gaillard
authors.forename: M
authors.initials: M
authors.lastname: Rottman
mesh_terms: D002177:Candidiasis / Q000175:diagnosis* / Q000209:etiology; D018929:Cell Culture Techniques; D005260:Female; D016905:Gram-Negative Bacterial Infections / Q000175:diagnosis* / Q000209:etiology; D016908:Gram-Positive Bacterial Infections / Q000175:diagnosis* / Q000209:etiology; D006801:Humans; D007595:Joint Prosthesis / Q000009:adverse effects* / Q000382:microbiology; D008297:Male; D008828:Microbiological Techniques; D008875:Middle Aged; D016459:Prosthesis-Related Infections / Q000175:diagnosis* / Q000209:etiology