Amyloid formation and disaggregation of α-synuclein and its tandem repeat (α-TR).
The aggregation of α-synuclein is clearly related to the pathogenesis of Parkinson's disease. Therefore, detailed understanding of the mechanism of fibril formation is highly valuable for the development of clinical treatment and also of the diagnostic tools. Here, we have investigated the interaction of α-synuclein with ionic liquids by using several biochemical techniques including Thioflavin T assays and transmission electron microscopy (TEM). Our data shows a rapid formation of α-synuclein amyloid fibrils was stimulated by 1-butyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide [BIMbF(3)Im], and these fibrils could be disaggregated by polyphenols such as epigallocatechin gallate (EGCG) and baicalein. Furthermore, the effect of [BIMbF(3)Im] on the α-synuclein tandem repeat (α-TR) in the aggregation process was studied.
Bae
Song Yi
SY
Department of Molecular Science and Technology, Graduate School of Interdisciplinary Programs, Ajou University, Suwon 443-749, South Korea.
Kim
Seulgi
S
Hwang
Heejin
H
Kim
Hyun-Kyung
HK
Yoon
Hyun C
HC
Kim
Jae Ho
JH
Lee
Sangyoon
S
Kim
T Doohun
TD
D000682:Amyloid / Q000737:chemistry / Q000378:metabolism*; D052160:Benzothiazoles; D002392:Catechin / Q000031:analogs & derivatives / Q000494:pharmacology; D044950:Flavanones / Q000494:pharmacology; D006801:Humans; D007093:Imidazoles / Q000494:pharmacology*; D007094:Imides / Q000494:pharmacology*; D052578:Ionic Liquids / Q000737:chemistry; D046529:Microscopy, Electron, Transmission; D010300:Parkinson Disease / Q000378:metabolism*; D013449:Sulfonamides; D020080:Tandem Repeat Sequences; D013844:Thiazoles / Q000737:chemistry; D051844:alpha-Synuclein / Q000737:chemistry / Q000378:metabolism*
C493485:1-butyl-3-methylimidazolium bis((trifluoromethyl)sulfonyl)amide; D000682:Amyloid; D052160:Benzothiazoles; D044950:Flavanones; D007093:Imidazoles; D007094:Imides; D052578:Ionic Liquids; D013449:Sulfonamides; D013844:Thiazoles; D051844:alpha-Synuclein; C009462:thioflavin T; C006680:baicalein; D002392:Catechin; C045651:epigallocatechin gallate