Ethanol induces mouse spermatogenic cell apoptosis in vivo through over-expression of Fas/Fas-L, p53, and caspase-3 along with cytochrome c translocation and glutathione depletion.
Although it has been well established that spermatogenic cells undergo apoptosis when treated with ethanol, the molecular mechanisms behind it remain to be investigated. Adult male mice were given intra-peritoneal injection (IP) of ethanol at a dose of 3 g (15%, v/v) per kg body weight per day during the period of 14 days. Testicular androgenesis and apoptotic germ cell death, along with different interrelated proteins expression, were evaluated. Ethanol treatment induced apoptotic spermatogenic cell death with a decrease in the plasma and intra-testicular testosterone concentration. Western blot analysis revealed that repeated ethanol treatment decreased the expression of steroidogenic acute regulatory protein (StAR), 3 beta-hydroxysteroid dehydrogenase (3beta-HSD) and 17 beta-hydroxysteroid dehydrogenase (17beta-HSD); increased the expression of active caspase-3, p53, Fas and Fas-L; and led to up-regulation of Bax/Bcl-2 ratio and translocation of cytochrome c from mitochondria to cytosol in testis. It has also been shown in our study that repeated ethanol treatment led to up-regulation of caspase-3, p53, Fas, Fas-L transcripts; increase in caspase-3 and caspase-8 activities; diminution of 3beta-HSD, 17beta-HSD, and GPx activities; decrease in the mitochondrial membrane potential along with ROS generation and depletion of glutathione pool in the testicular tissue. The present study has indicated that the ethanol treatment induced apoptosis in the mouse testis through the increased expression of Fas/Fas-L and p53, up-regulation of Bax/Bcl-2 ratio, cytosolic translocation of cytochrome c along with caspase-3 activation and glutathione depletion.
Jana
Kuladip
K
Division of Molecular Medicine, Bose Institute, Kolkata, West Bengal, India. kuladip@bic.boseinst.ernet.in
Jana
Narayan
N
De
Dipak Kumar
DK
Guha
Sujoy Kumar
SK
D015067:17-Hydroxysteroid Dehydrogenases / Q000032:analysis; D015096:3-Hydroxysteroid Dehydrogenases / Q000032:analysis; D000818:Animals; D017209:Apoptosis; D053148:Caspase 3 / Q000032:analysis; D045304:Cytochromes c / Q000032:analysis; D015536:Down-Regulation; D000431:Ethanol / Q000494:pharmacology*; D053222:Fas Ligand Protein / Q000032:analysis; D005978:Glutathione / Q000032:analysis; D008297:Male; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D051379:Mice; D010750:Phosphoproteins / Q000032:analysis; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis; D017382:Reactive Oxygen Species / Q000032:analysis; D013091:Spermatogenesis / Q000187:drug effects*; D013094:Spermatozoa / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D013737:Testis / Q000187:drug effects* / Q000378:metabolism; D013739:Testosterone / Q000096:biosynthesis / Q000097:blood; D016159:Tumor Suppressor Protein p53 / Q000032:analysis; D015854:Up-Regulation; D051028:bcl-2-Associated X Protein / Q000032:analysis; D000098682:Steroidogenic Acute Regulatory Protein
D053222:Fas Ligand Protein; D010750:Phosphoproteins; D019253:Proto-Oncogene Proteins c-bcl-2; D017382:Reactive Oxygen Species; D016159:Tumor Suppressor Protein p53; D051028:bcl-2-Associated X Protein; D000098682:Steroidogenic Acute Regulatory Protein; D000431:Ethanol; D013739:Testosterone; D045304:Cytochromes c; D015067:17-Hydroxysteroid Dehydrogenases; D015096:3-Hydroxysteroid Dehydrogenases; D053148:Caspase 3; D005978:Glutathione