title: Microarray-based approach identifies differentially expressed microRNAs in porcine sexually immature and mature testes.
abstract: BACKGROUND
  MicroRNAs (miRNAs) are short non-coding RNA molecules which are proved to be involved in mammalian spermatogenesis. Their expression and function in the porcine germ cells are not fully understood.
  
  
  METHODS
  We employed a miRNA microarray containing 1260 unique miRNA probes to evaluate the miRNA expression patterns between sexually immature (60-day) and mature (180-day) pig testes. One hundred and twenty nine miRNAs representing 164 reporter miRNAs were expressed differently (p<0.1). Fifty one miRNAs were significantly up-regulated and 78 miRNAs were down-regulated in mature testes. Nine of these differentially expressed miRNAs were validated using quantitative RT-PCR assay. Totally 15,919 putative miRNA-target sites were detected by using RNA22 method to align 445 NCBI pig cDNA sequences with these 129 differentially expressed miRNAs, and seven putative target genes involved in spermatogenesis including DAZL, RNF4 gene were simply confirmed by quantitative RT-PCR.
  
  
  CONCLUSIONS
  Overall, the results of this study indicated specific miRNAs expression in porcine testes and suggested that miRNAs had a role in regulating spermatogenesis.
authors.affiliation: Key Laboratory of Pig Genetics and Breeding of Ministry of Agriculture, Huazhong Agricultural University, Wuhan, People's Republic of China.
authors.forename: Lifan
authors.initials: L
authors.lastname: Luo
authors.forename: Lianzhi
authors.initials: L
authors.lastname: Ye
authors.forename: Gang
authors.initials: G
authors.lastname: Liu
authors.forename: Guochao
authors.initials: G
authors.lastname: Shao
authors.forename: Rong
authors.initials: R
authors.lastname: Zheng
authors.forename: Zhuqing
authors.initials: Z
authors.lastname: Ren
authors.forename: Bo
authors.initials: B
authors.lastname: Zuo
authors.forename: Dequan
authors.initials: D
authors.lastname: Xu
authors.forename: Minggang
authors.initials: M
authors.lastname: Lei
authors.forename: Siwen
authors.initials: S
authors.lastname: Jiang
authors.forename: Changyan
authors.initials: C
authors.lastname: Deng
authors.forename: Yuanzhu
authors.initials: Y
authors.lastname: Xiong
authors.forename: Fenge
authors.initials: F
authors.lastname: Li
mesh_terms: D000818:Animals; D019295:Computational Biology; D020869:Gene Expression Profiling; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D015203:Reproducibility of Results; D012741:Sexual Maturation / Q000235:genetics*; D013091:Spermatogenesis / Q000235:genetics; D013552:Swine / Q000235:genetics* / Q000502:physiology*; D013737:Testis / Q000378:metabolism*
chemical_list: D035683:MicroRNAs
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