title: Transcription factor Stb5p is essential for acetaldehyde tolerance in Saccharomyces cerevisiae.
abstract: Transcription factor Stb5p, previously known as one of the multidrug resistance gene regulators in Saccharomyces cerevisiae, was shown here to play an essential role in acetaldehyde tolerance. A mutant strain, Δstb5 exhibited increased acetaldehyde sensitivity, and failed to induce genes such as GND1, TKL1 and TAL1 involved in the pentose phosphate pathway (PPP) upon acetaldehyde stress. Using this strain it was revealed that Stb5p acts as a repressor for PGI1 encoding glucose-6-phosphate isomerase under acetaldehyde stress. In reverse, over-expression of Stb5p reinforced acetaldehyde tolerance to the yeast. Furthermore, various deletion mutants of the genes involved in glycolysis showed increased acetaldehyde tolerance compared to the wild-type strain. From these results, it was suggested that Stb5p participates in acetaldehyde tolerance by regulating expression of the PPP genes and PGI1, and that down-regulation of glycolytic pathway may lead to vitalization of PPP and to increased acetaldehyde tolerance.
authors.affiliation: Department of Food Science and Technology, Faculty of Bioindustry, Tokyo University of Agriculture, Abashiri, Hokkaido, Japan.
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Matsufuji
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Nakagawa
authors.forename: Shuki
authors.initials: S
authors.lastname: Fujimura
authors.forename: Akio
authors.initials: A
authors.lastname: Tani
authors.forename: Junichi
authors.initials: J
authors.lastname: Nakagawa
mesh_terms: D000079:Acetaldehyde / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D005956:Glucose-6-Phosphate Isomerase / Q000378:metabolism; D010427:Pentose Phosphate Pathway; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D029701:Saccharomyces cerevisiae Proteins; C488251:Stb5 protein, S cerevisiae; D014157:Transcription Factors; D005956:Glucose-6-Phosphate Isomerase; D000079:Acetaldehyde