Patterning of peptide nucleic acids using reactive microcontact printing.
PNAs (peptide nucleic acids) have been immobilized onto surfaces in a fast, accurate way by employing reactive microcontact printing. Surfaces have been first modified with aldehyde groups to react with the amino end of the synthesized PNAs. When patterning fluorescein-labeled PNAs by reactive microcontact printing using oxygen-oxidized polydimethylsiloxane stamps, homogeneous arrays were fabricated and characterized using optical methods. PNA-patterned surfaces were hybridized with complementary and mismatched dye-labeled oligonucleotides to test their ability to recognize DNA sequences. The stability and selectivity of the PNA-DNA duplexes on surfaces have been verified by fluorescence microscopy, and the melting curves have been recorded. Finally, the technique has been applied to the fabrication of chips by spotting a PNA microarray onto a flat PDMS stamp and reproducing the same features onto many slides. The chips were finally applied to single nucleotide polymorphism detection on oligonucleotides.
Calabretta
Alessandro
A
Molecular Nanofabrication and Biophysical Engineering, Department of Science and Technology, University of Twente, PEnschede, The Netherlands.
Wasserberg
Dorothee
D
Posthuma-Trumpie
Geertruida A
GA
Subramaniam
Vinod
V
van Amerongen
Aart
A
Corradini
Roberto
R
Tedeschi
Tullia
T
Sforza
Stefano
S
Reinhoudt
David N
DN
Marchelli
Rosangela
R
Huskens
Jurriaan
J
Jonkheijm
Pascal
P
D008856:Microscopy, Fluorescence; D020135:Peptide Nucleic Acids / Q000737:chemistry*
D020135:Peptide Nucleic Acids