Chitosan/siRNA nanoparticles biofunctionalize nerve implants and enable neurite outgrowth.
Microstructured 20 μm thick polymer filaments used as nerve implants were loaded with chitosan/siRNA nanoparticles to promote nerve regeneration and ensure local delivery of nanotherapeutics. The stable nanoparticles were rapidly internalized by cells and did not affect cell viability. Target mRNA was successfully reduced by 65-75% and neurite outgrowth was enhanced even in an inhibitory environment. This work, thus, supports the application of nanobiofunctionalized implants as a novel approach for spinal cord and nerve repair.
Mittnacht
Ursula
U
NMI Natural and Medical Sciences Institute at the University of Tübingen, Markwiesenstrasse 55, 72770 Reutlingen, Germany.
Hartmann
Hanna
H
Hein
San
S
Oliveira
Hugo
H
Dong
Mingdong
M
Pêgo
Ana P
AP
Kjems
Jørgen
J
Howard
Kenneth A
KA
Schlosshauer
Burkhard
B
D000818:Animals; D002460:Cell Line; D048271:Chitosan / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D009416:Nerve Regeneration; D016501:Neurites / Q000378:metabolism; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D019736:Prostheses and Implants / Q000648:ultrastructure; D034741:RNA, Small Interfering / Q000008:administration & dosage* / Q000235:genetics; D014162:Transfection; D020742:rhoA GTP-Binding Protein / Q000235:genetics
D034741:RNA, Small Interfering; D048271:Chitosan; D020742:rhoA GTP-Binding Protein