title: Proteomic signatures uncover hydrogen peroxide and nitric oxide cross-talk signaling network in citrus plants.
abstract: Hydrogen peroxide (H(2)O(2)) and nitric oxide ((•)NO) elicit numerous processes in plants. However, our knowledge of H(2)O(2) and (•)NO-responsive proteins is limited. The present study aimed to identify proteins whose accumulation levels were regulated by these signaling molecules in citrus leaves. To address this question, hydroponically grown citrus plants were treated by incubating their roots in the presence of H(2)O(2) or the (•)NO donor, sodium nitroprusside (SNP). Both treatments induced H(2)O(2) and (•)NO production in leaves, indicating occurrence of oxidative and nitrosative stress conditions. However, treated plants maintained their normal physiological status. The vascular system was shown to be involved in the H(2)O(2) and (•)NO systemic signaling as evidenced by real-time labeling of the two molecules. Comparative proteomic analysis identified a number of proteins whose accumulation levels were altered by treatments. They were mainly involved in photosynthesis, defense and energy. More than half of them were commonly modulated by both treatments, indicating a strong overlap between H(2)O(2) and (•)NO responses. Using a redox proteomic approach, several proteins were also identified as being carbonylation targets of H(2)O(2) and SNP. The analysis reveals an interlinked H(2)O(2) and (•)NO proteins network allowing a deeper understanding of oxidative and nitrosative signaling in plants.
authors.affiliation: Aristotle University of Thessaloniki, School of Agriculture, University Campus, 54124 Thessaloniki, Greece. gtanou@agro.auth.gr
authors.forename: Georgia
authors.initials: G
authors.lastname: Tanou
authors.forename: Claudette
authors.initials: C
authors.lastname: Job
authors.forename: Maya
authors.initials: M
authors.lastname: Belghazi
authors.forename: Athanassios
authors.initials: A
authors.lastname: Molassiotis
authors.forename: Grigorios
authors.initials: G
authors.lastname: Diamantidis
authors.forename: Dominique
authors.initials: D
authors.lastname: Job
mesh_terms: D002957:Citrus / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism*; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D018515:Plant Leaves / Q000378:metabolism; D010940:Plant Proteins / Q000032:analysis*; D040901:Proteomics / Q000379:methods; D020239:Receptor Cross-Talk; D015398:Signal Transduction
chemical_list: D010940:Plant Proteins; D009569:Nitric Oxide; D006861:Hydrogen Peroxide