title: Time-dependent proteomic iTRAQ analysis of nasal lavage of hairdressers challenged by persulfate.
abstract: Hairdressers are frequently exposed to bleaching powder containing persulfates, a group of compounds that may induce hypersensitivity in the airways. The mechanism causing this reaction is not clear. The aim of this study was to identify changes in the nasal lavage fluid proteome after challenge with potassium persulfate in hairdressers with bleaching powder-associated rhinitis. Furthermore, we aimed to compare their response to that of hairdressers without nasal symptoms, and atopic subjects with pollen-associated nasal symptoms. To study the pathogenesis of persulfate-associated rhinitis, the response in protein expression from the upper airway was assessed by time-dependent proteomic expression analysis of nasal lavage fluids. Samples were prepared by pooling nasal lavage fluids from the groups at different time points after challenge. Samples were depleted of high-abundant proteins, labeled with iTRAQ and analyzed by online 2D-nanoLC-MS/MS. Differences in the protein pattern between the three groups were observed. Most proteins with differentially expressed levels were involved in pathways of lipid transportation and antimicrobial activities. The major finding was increased abundance of apolipoprotein A-1, 20 min postchallenge, detected solely in the group of symptomatic hairdressers. Our results suggest there may be differences between the mechanisms responsible for the rhinitis in the symptomatic and atopic group.
authors.affiliation: Department of Laboratory Medicine, Lund University, SE-221 85 Lund, Sweden.
authors.forename: Monica H
authors.initials: MH
authors.lastname: Kåredal
authors.forename: Harriet
authors.initials: H
authors.lastname: Mortstedt
authors.forename: Marina C
authors.initials: MC
authors.lastname: Jeppsson
authors.forename: Kerstin
authors.initials: K
authors.lastname: Kronholm Diab
authors.forename: Jørn
authors.initials: J
authors.lastname: Nielsen
authors.forename: Bo A G
authors.initials: BA
authors.lastname: Jonsson
authors.forename: Christian H
authors.initials: CH
authors.lastname: Lindh
mesh_terms: D016632:Apolipoprotein A-I / Q000096:biosynthesis; D005260:Female; D015870:Gene Expression / Q000276:immunology; D058206:Hair Bleaching Agents / Q000009:adverse effects*; D006801:Humans; D007700:Kinetics; D017817:Nasal Lavage Fluid / Q000737:chemistry*; D016273:Occupational Exposure / Q000009:adverse effects*; D017680:Potassium Compounds / Q000276:immunology*; D011506:Proteins / Q000032:analysis*; D020543:Proteome / Q000032:analysis / Q000276:immunology*; D012220:Rhinitis / Q000139:chemically induced*; D013431:Sulfates / Q000276:immunology*
chemical_list: D016632:Apolipoprotein A-I; D058206:Hair Bleaching Agents; D017680:Potassium Compounds; D011506:Proteins; D020543:Proteome; D013431:Sulfates; C009007:potassium persulfate