title: Comparison of transcriptional and translational changes caused by long-term menadione exposure in Aspergillus nidulans.
abstract: Under long-term oxidative stress caused by menadione sodium bisulfite, genome-wide transcriptional and proteome-wide translational changes were compared in Aspergillus nidulans vegetative cells. The comparison of proteomic and DNA microarray expression data demonstrated that global gene expression changes recorded with either flip-flop or dendrimer cDNA labeling techniques supported proteome changes moderately with 40% and 34% coincidence coefficients, respectively. Enzyme levels in the glycolytic pathway were alternating, which was a direct consequence of fluctuating gene expression patterns. Surprisingly, enzymes in the vitamin B2 and B6 biosynthetic pathways were repressed concomitantly with the repression of some protein folding chaperones and nuclear transport elements. Under long-term oxidative stress, the peroxide-detoxifying peroxiredoxins and cytochrome c peroxidase were replaced by thioredoxin reductase, a nitroreductase and a flavohemoprotein, and protein degradation became predominant to eliminate damaged proteins.
authors.affiliation: Department of Microbial Biotechnology and Cell Biology, Faculty of Science and Technology, University of Debrecen, Egyetem tér 1, H-4032 Debrecen, Hungary. pusztahelyi@yahoo.com
authors.forename: Tünde
authors.initials: T
authors.lastname: Pusztahelyi
authors.forename: Eva
authors.initials: E
authors.lastname: Klement
authors.forename: Emilia
authors.initials: E
authors.lastname: Szajli
authors.forename: József
authors.initials: J
authors.lastname: Klem
authors.forename: Márton
authors.initials: M
authors.lastname: Miskei
authors.forename: Zsolt
authors.initials: Z
authors.lastname: Karányi
authors.forename: Tamás
authors.initials: T
authors.lastname: Emri
authors.forename: Szilvia
authors.initials: S
authors.lastname: Kovács
authors.forename: Gyula
authors.initials: G
authors.lastname: Orosz
authors.forename: Kornél L
authors.initials: KL
authors.lastname: Kovács
authors.forename: Katalin F
authors.initials: KF
authors.lastname: Medzihradszky
authors.forename: Rolf A
authors.initials: RA
authors.lastname: Prade
authors.forename: István
authors.initials: I
authors.lastname: Pócsi
mesh_terms: D001233:Aspergillus nidulans / Q000187:drug effects*; D020869:Gene Expression Profiling; D053858:Metabolic Networks and Pathways / Q000235:genetics; D046228:Microarray Analysis; D016877:Oxidants / Q000378:metabolism*; D014176:Protein Biosynthesis / Q000187:drug effects*; D020543:Proteome / Q000032:analysis; D014158:Transcription, Genetic / Q000187:drug effects*; D024483:Vitamin K 3 / Q000378:metabolism*
chemical_list: D016877:Oxidants; D020543:Proteome; D024483:Vitamin K 3