title: Expression of inflammation-related cytokines following intratracheal instillation of nickel oxide nanoparticles.
abstract: The objective of this study was to examine what kinds of cytokines are related to lung disorder by well-dispersed nanoparticles. The mass median diameter of nickel oxide in distilled water was 26 nm. Rats intratracheally received 0.2 mg of nickel oxide suspended in distilled water, and were sacrificed from three days to six months. The concentrations of 21 cytokines including inflammation, fibrosis and allergy-related ones were measured in the lung. Infiltration of alveolar macrophages was observed persistently in the nickel oxide-exposed group. Expression of macrophage inflammatory protein-1alpha showed a continued increase in lung tissue and broncho-alveolar lavage fluid (BALF) while interleukin-1alpha (IL-1alpha), IL-1beta in lung tissue and monocyte chemotactic protein-1 in BALF showed transient increases. Taken together, it was suggested that nano-agglomerates of nickel oxide nanoparticles have a persistent inflammatory effect, and the transient increase in cytokine expression and persistent increases in CC chemokine were involved in the persistent pulmonary inflammation.
authors.affiliation: Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, Kitakyushu, Fukuoka, Japan. yasuom@med.uoeh-u.ac.jp
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Morimoto
authors.forename: Akira
authors.initials: A
authors.lastname: Ogami
authors.forename: Motoi
authors.initials: M
authors.lastname: Todoroki
authors.forename: Makoto
authors.initials: M
authors.lastname: Yamamoto
authors.forename: Masahiro
authors.initials: M
authors.lastname: Murakami
authors.forename: Masami
authors.initials: M
authors.lastname: Hirohashi
authors.forename: Takako
authors.initials: T
authors.lastname: Oyabu
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Myojo
authors.forename: Ken-Ichiro
authors.initials: K
authors.lastname: Nishi
authors.forename: Chikara
authors.initials: C
authors.lastname: Kadoya
authors.forename: Sayumi
authors.initials: S
authors.lastname: Yamasaki
authors.forename: Hiroko
authors.initials: H
authors.lastname: Nagatomo
authors.forename: Katsuhide
authors.initials: K
authors.lastname: Fujita
authors.forename: Shigehisa
authors.initials: S
authors.lastname: Endoh
authors.forename: Kunio
authors.initials: K
authors.lastname: Uchida
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Yamamoto
authors.forename: Norihiro
authors.initials: N
authors.lastname: Kobayashi
authors.forename: Junko
authors.initials: J
authors.lastname: Nakanishi
authors.forename: Isamu
authors.initials: I
authors.lastname: Tanaka
mesh_terms: D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology / Q000276:immunology; D016207:Cytokines / Q000096:biosynthesis* / Q000276:immunology; D004195:Disease Models, Animal; D007322:Instillation, Drug; D007442:Intubation, Intratracheal; D008168:Lung / Q000187:drug effects* / Q000276:immunology / Q000648:ultrastructure; D016676:Macrophages, Alveolar / Q000166:cytology / Q000276:immunology; D008297:Male; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000633:toxicity*; D009532:Nickel / Q000633:toxicity*; D010316:Particle Size; D011014:Pneumonia / Q000209:etiology* / Q000276:immunology / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar
chemical_list: D016207:Cytokines; D009532:Nickel; C028007:nickel monoxide