title: CLE peptides can negatively regulate protoxylem vessel formation via cytokinin signaling.
abstract: Cell-cell communication is critical for tissue and organ development. In plants, secretory CLAVATA3/EMBRYO SURROUNDING REGION-related (CLE) peptides function as intercellular signaling molecules in various aspects of tissue development including vascular development. However, little is known about intracellular signaling pathways functioning in vascular development downstream of the CLE ligands. We show that CLE peptides including CLE10, which is preferentially expressed in the root vascular system, inhibit protoxylem vessel formation in Arabidopsis roots. GeneChip analysis displayed that CLE10 peptides repressed specifically the expression of two type-A Arabidopsis Response Regulators (ARRs), ARR5 and ARR6, whose products act as negative regulators of cytokinin signaling. The arr5 arr6 roots exhibited defective protoxylem vessel formation. These results indicate that CLE10 inhibits protoxylem vessel formation by suppressing the expression of type-A ARR genes including ARR5 and ARR6. This was supported by the finding that CLE10 did not suppress protoxylem vessel formation in a background of arr10 arr12, a double mutant of type-B ARR genes. Thus, our results revealed cross-talk between CLE signaling and cytokinin signaling in protoxylem vessel formation in roots. Taken together with the indication that cytokinin signaling functions downstream of the CLV3/WUS signaling pathway in the shoot apical meristem, the cross-talk between CLE and cytokinin signaling pathways may be a common feature in plant development.
authors.affiliation: Department of Biological Sciences, Graduate School of Science, University of Tokyo, Tokyo, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Kondo
authors.forename: Yuki
authors.initials: Y
authors.lastname: Hirakawa
authors.forename: Joseph J
authors.initials: JJ
authors.lastname: Kieber
authors.forename: Hiroo
authors.initials: H
authors.lastname: Fukuda
mesh_terms: D003583:Cytokinins / Q000502:physiology*; D010446:Peptide Fragments / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry / Q000502:physiology*; D015398:Signal Transduction / Q000502:physiology*; D052584:Xylem / Q000502:physiology*
chemical_list: D003583:Cytokinins; D010446:Peptide Fragments; D010940:Plant Proteins
references.citation: Plant Cell. 2008 Apr;20(4):934-46
references.pmid: 18381924
references.citation: Genes Dev. 2000 Dec 1;14(23):2938-43
references.pmid: 11114883
references.citation: Annu Rev Plant Physiol Plant Mol Biol. 2001 Jun;52:89-118
references.pmid: 11337393
references.citation: Genes Dev. 2005 Aug 15;19(16):1855-60
references.pmid: 16103214
references.citation: Plant Mol Biol. 2003 Sep;53(1-2):247-59
references.pmid: 14756321
references.citation: Plant Cell. 2008 Jun;20(6):1494-503
references.pmid: 18523061
references.citation: Science. 2006 Aug 11;313(5788):842-5
references.pmid: 16902140
references.citation: Plant Physiol. 2002 Jun;129(2):565-75
references.pmid: 12068101
references.citation: Plant Cell Physiol. 2001 Sep;42(9):1017-23
references.pmid: 11577198
references.citation: Proc Natl Acad Sci U S A. 2008 Dec 16;105(50):20027-31
references.pmid: 19074290
references.citation: Cell. 2000 Mar 17;100(6):635-44
references.pmid: 10761929
references.citation: Plant Cell Physiol. 2003 Aug;44(8):868-74
references.pmid: 12941880
references.citation: Plant Cell Physiol. 2001 Jul;42(7):677-85
references.pmid: 11479373
references.citation: Trends Plant Sci. 2009 May;14(5):255-63
references.pmid: 19362511
references.citation: Plant Cell. 2007 Dec;19(12):3901-14
references.pmid: 18065689
references.citation: Plant Physiol. 2010 Jan;152(1):166-76
references.pmid: 19933383
references.citation: Plant Cell. 2009 Jul;21(7):2008-21
references.pmid: 19622803
references.citation: Curr Biol. 2007 Jun 19;17(12):1061-6
references.pmid: 17570668
references.citation: Proc Natl Acad Sci U S A. 2008 Sep 30;105(39):15208-13
references.pmid: 18812507
references.citation: Science. 2006 Jan 6;311(5757):94-8
references.pmid: 16400151
references.citation: Nat Rev Mol Cell Biol. 2004 May;5(5):379-91
references.pmid: 15122351
references.citation: Plant Physiol. 2003 Oct;133(2):462-9
references.pmid: 14555774
references.citation: Plant Cell Physiol. 2007 Jan;48(1):84-96
references.pmid: 17132632
references.citation: Nature. 2010 May 20;465(7296):316-21
references.pmid: 20410882
references.citation: Plant J. 2008 Aug;55(4):652-64
references.pmid: 18445131
references.citation: Nat Chem Biol. 2009 Aug;5(8):578-80
references.pmid: 19525968
references.citation: Plant Cell. 2005 Nov;17(11):3007-18
references.pmid: 16227453
references.citation: Proc Natl Acad Sci U S A. 2008 Dec 16;105(50):20032-7
references.pmid: 19064928
references.citation: Plant Cell Physiol. 2007 Dec;48(12):1821-5
references.pmid: 17991631
references.citation: Plant Cell Physiol. 2006 Oct;47(10):1394-405
references.pmid: 16980704
references.citation: Cell Res. 2009 Oct;19(10):1178-90
references.pmid: 19621034
references.citation: Plant Cell. 2004 Mar;16(3):658-71
references.pmid: 14973166
references.citation: Plant Cell. 2002;14 Suppl:S47-59
references.pmid: 12045269
references.citation: Nature. 2001 Feb 22;409(6823):1060-3
references.pmid: 11234017
references.citation: Plant Physiol. 2004 Jun;135(2):927-37
references.pmid: 15173562
references.citation: Gene. 2004 Feb 18;327(1):37-49
references.pmid: 14960359
references.citation: Plant Cell. 2008 Aug;20(8):2102-16
references.pmid: 18723577
references.citation: Nature. 2005 Dec 22;438(7071):1172-5
references.pmid: 16372013
references.citation: Plant J. 2010 Jan;61(2):223-33
references.pmid: 19843317
references.citation: Plant Cell Physiol. 2001 Jul;42(7):751-5
references.pmid: 11479382
references.citation: Plant Cell Physiol. 2001 Feb;42(2):107-13
references.pmid: 11230563
references.citation: Plant Cell Physiol. 2008 Nov;49(11):1752-7
references.pmid: 18854335
references.citation: J Biol Chem. 2001 Jul 13;276(28):26405-10
references.pmid: 11313355
references.citation: Proc Natl Acad Sci U S A. 2008 Nov 25;105(47):18625-30
references.pmid: 19011104
references.citation: Plant Cell. 2009 Mar;21(3):736-48
references.pmid: 19258439
references.citation: Plant Cell Physiol. 2008 Jan;49(1):47-57
references.pmid: 18037673
references.citation: Cell Mol Life Sci. 2008 Mar;65(5):743-55
references.pmid: 18034320