title: Selection of naturally occurring autoantibodies to interleukin-18 from phage display library.
abstract: Four unique phage single-chain variable fragments (scFvs) to recombinant human interleukin 18 (IL-18) has been selected from a naïve combinatorial library of human scFvs. Binding of unique phage antibodies with IL-18 was tested by ELISA and Western-blotting. No cross reactivity with tumor necrosis factor α, interferons α and γ was shown for the selected antibodies. The gene segments encoding V(D)J regions of selected antibodies exhibited a high degree of homology to germline genes, therefore we suggest that the selected scFv's belong to repertoire of naïve autoantibodies.
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Sciences, Novosibirsk, Russia.
authors.forename: M A
authors.initials: MA
authors.lastname: Vikhrova
authors.forename: M V
authors.initials: MV
authors.lastname: Shveygert
authors.forename: E A
authors.initials: EA
authors.lastname: Khrapov
authors.forename: M L
authors.initials: ML
authors.lastname: Filipenko
authors.forename: I P
authors.initials: IP
authors.lastname: Gileva
authors.forename: V V
authors.initials: VV
authors.lastname: Morozova
authors.forename: N V
authors.initials: NV
authors.lastname: Tikunova
mesh_terms: D000595:Amino Acid Sequence; D001323:Autoantibodies / Q000235:genetics / Q000276:immunology*; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D007147:Immunoglobulin Light Chains / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D020382:Interleukin-18 / Q000235:genetics / Q000276:immunology*; D008969:Molecular Sequence Data; D019151:Peptide Library; D012150:Polymorphism, Restriction Fragment Length; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology*; D057127:Single-Chain Antibodies / Q000235:genetics / Q000276:immunology*
chemical_list: D001323:Autoantibodies; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D020382:Interleukin-18; D019151:Peptide Library; D011994:Recombinant Proteins; D057127:Single-Chain Antibodies