title: Alpha-lipoic acid modulates GFAP, vimentin, nestin, cyclin D1 and MAP-kinase expression in astroglial cell cultures.
abstract: In the present study, we evaluated the expression of some proliferation and differentiation markers in 15 DIV astrocyte cultures pretreated or not with 0.5 mM glutamate for 24 h and than maintained under chronic or acute treatment with 50 μM R(+)enantiomer or raceme alpha-lipoic acid (ALA). GFAP expression significantly increased after (R+)enantiomer acute-treatment and also in glutamate-pretreated ones. Vimentin expression increased after R(+)enantiomer acute-treatment, but it decreased after raceme acute-treatment. Nestin expression drastically increased after acute raceme-treatment in glutamate-pretreated or not cultures, but significantly decreased after (R+)enantiomer acute and chronic-treatments. Cyclin D1 expression increased in raceme acute-treated cultures pretreated with glutamate. MAP-kinase expression slightly increased after (R+)enantiomer acute treatment in glutamate-pretreated or unpretreated ones. These preliminary findings may better clarify antioxidant and metabolic role played by ALA in proliferating and differentiating astrocyte cultures suggesting an interactive cross-talk between glial and neuronal cells, after brain lesions or damages.
authors.affiliation: Dept of Chemical Sciences, Section of Biochemistry and Molecular biology, University of Catania, Viale A Doria 6, 95125 Catania, Italy.
authors.forename: V
authors.initials: V
authors.lastname: Bramanti
authors.forename: D
authors.initials: D
authors.lastname: Tomassoni
authors.forename: D
authors.initials: D
authors.lastname: Bronzi
authors.forename: S
authors.initials: S
authors.lastname: Grasso
authors.forename: M
authors.initials: M
authors.lastname: Currò
authors.forename: M
authors.initials: M
authors.lastname: Avitabile
authors.forename: G
authors.initials: G
authors.lastname: Li Volsi
authors.forename: M
authors.initials: M
authors.lastname: Renis
authors.forename: R
authors.initials: R
authors.lastname: Ientile
authors.forename: F
authors.initials: F
authors.lastname: Amenta
authors.forename: R
authors.initials: R
authors.lastname: Avola
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D015153:Blotting, Western; D002478:Cells, Cultured; D019938:Cyclin D1 / Q000378:metabolism*; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism*; D007381:Intermediate Filament Proteins / Q000378:metabolism*; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D064231:Nestin; D051381:Rats; D017208:Rats, Wistar; D008063:Thioctic Acid / Q000494:pharmacology*; D014746:Vimentin / Q000378:metabolism*
chemical_list: D005904:Glial Fibrillary Acidic Protein; D007381:Intermediate Filament Proteins; D009419:Nerve Tissue Proteins; C577486:Nes protein, rat; D064231:Nestin; D014746:Vimentin; D019938:Cyclin D1; D008063:Thioctic Acid; D020928:Mitogen-Activated Protein Kinases
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