Expression of inflammation-related cytokines following intratracheal instillation of nickel oxide nanoparticles.
The objective of this study was to examine what kinds of cytokines are related to lung disorder by well-dispersed nanoparticles. The mass median diameter of nickel oxide in distilled water was 26 nm. Rats intratracheally received 0.2 mg of nickel oxide suspended in distilled water, and were sacrificed from three days to six months. The concentrations of 21 cytokines including inflammation, fibrosis and allergy-related ones were measured in the lung. Infiltration of alveolar macrophages was observed persistently in the nickel oxide-exposed group. Expression of macrophage inflammatory protein-1alpha showed a continued increase in lung tissue and broncho-alveolar lavage fluid (BALF) while interleukin-1alpha (IL-1alpha), IL-1beta in lung tissue and monocyte chemotactic protein-1 in BALF showed transient increases. Taken together, it was suggested that nano-agglomerates of nickel oxide nanoparticles have a persistent inflammatory effect, and the transient increase in cytokine expression and persistent increases in CC chemokine were involved in the persistent pulmonary inflammation.
Morimoto
Yasuo
Y
Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, Kitakyushu, Fukuoka, Japan. yasuom@med.uoeh-u.ac.jp
Ogami
Akira
A
Todoroki
Motoi
M
Yamamoto
Makoto
M
Murakami
Masahiro
M
Hirohashi
Masami
M
Oyabu
Takako
T
Myojo
Toshihiko
T
Nishi
Ken-Ichiro
K
Kadoya
Chikara
C
Yamasaki
Sayumi
S
Nagatomo
Hiroko
H
Fujita
Katsuhide
K
Endoh
Shigehisa
S
Uchida
Kunio
K
Yamamoto
Kazuhiro
K
Kobayashi
Norihiro
N
Nakanishi
Junko
J
Tanaka
Isamu
I
D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology / Q000276:immunology; D016207:Cytokines / Q000096:biosynthesis* / Q000276:immunology; D004195:Disease Models, Animal; D007322:Instillation, Drug; D007442:Intubation, Intratracheal; D008168:Lung / Q000187:drug effects* / Q000276:immunology / Q000648:ultrastructure; D016676:Macrophages, Alveolar / Q000166:cytology / Q000276:immunology; D008297:Male; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000633:toxicity*; D009532:Nickel / Q000633:toxicity*; D010316:Particle Size; D011014:Pneumonia / Q000209:etiology* / Q000276:immunology / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar
D016207:Cytokines; D009532:Nickel; C028007:nickel monoxide