title: Stress-induced alterations in mast cell numbers and proteinase-activated receptor-2 expression of the colon: role of corticotrophin-releasing factor.
abstract: This study was performed in order to assess whether acute stress can increase mast cell and enterochromaffin (EC) cell numbers, and proteinase-activated receptor-2 (PAR2) expression in the rat colon. In addition, we aimed to investigate the involvement of corticotrophin-releasing factor in these stress-related alterations. Eighteen adult rats were divided into 3 experimental groups: 1) a saline-pretreated non-stressed group, 2) a saline-pretreated stressed group, and 3) an astressin-pretreated stressed group. The numbers of mast cells, EC cells, and PAR2-positive cells were counted in 6 high power fields. In proximal colonic segments, mast cell numbers of stressed rats tended to be higher than those of non-stressed rats, and their PAR2-positive cell numbers were significantly higher than those of non-stressed rats. In distal colonic segments, mast cell numbers and PAR2-positive cell numbers of stressed rats were significantly higher than those of non-stressed rats. Mast cell and PAR2-positive cell numbers of astressin-pretreated stressed rats were significantly lower than those of saline-pretreated stressed rats. EC cell numbers did not differ among the three experimental groups. Acute stress in rats increases mast cell numbers and mucosal PAR2 expression in the colon. These stress-related alterations seem to be mediated by release of corticotrophin-releasing factor.
authors.affiliation: Department of Gastroenterology, Ajou University School of Medicine, Suwon, Korea. kjleemd@hotmail.com
authors.forename: Dong Hoon
authors.initials: DH
authors.lastname: Kim
authors.forename: Young Ju
authors.initials: YJ
authors.lastname: Cho
authors.forename: Jang Hee
authors.initials: JH
authors.lastname: Kim
authors.forename: Young Bae
authors.initials: YB
authors.lastname: Kim
authors.forename: Kwang Jae
authors.initials: KJ
authors.lastname: Lee
mesh_terms: D000818:Animals; D003106:Colon / Q000378:metabolism*; D003346:Corticotropin-Releasing Hormone / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000494:pharmacology / Q000502:physiology*; D004759:Enterochromaffin Cells / Q000166:cytology; D008297:Male; D008407:Mast Cells / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D010446:Peptide Fragments / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D044464:Receptor, PAR-2 / Q000378:metabolism*; D012149:Restraint, Physical; D013312:Stress, Physiological
chemical_list: D010446:Peptide Fragments; D044464:Receptor, PAR-2; C104416:astressin; D003346:Corticotropin-Releasing Hormone
references.citation: J Gastroenterol Hepatol. 2008 Nov;23(11):1689-94
references.pmid: 19120860
references.citation: J Gastroenterol. 2007 Jan;42 Suppl 17:48-51
references.pmid: 17238026
references.citation: Gastroenterology. 1997 May;112(5):1714-43
references.pmid: 9136853
references.citation: Gastroenterology. 2002 Apr;122(4):1035-47
references.pmid: 11910355
references.citation: Eur J Biochem. 1995 Aug 15;232(1):84-9
references.pmid: 7556175
references.citation: Endocrinology. 1990 Jul;127(1):440-52
references.pmid: 2163323
references.citation: Gut. 2008 May;57(5):591-9
references.pmid: 18194983
references.citation: Scand J Gastroenterol. 1997 Nov;32(11):1107-17
references.pmid: 9399391
references.citation: Am J Gastroenterol. 2003 Jul;98(7):1578-83
references.pmid: 12873581
references.citation: Am J Physiol. 1996 Nov;271(5 Pt 1):G884-92
references.pmid: 8944704
references.citation: Gut. 2005 Jun;54(6):867-74
references.pmid: 15888798
references.citation: J Neuroimmunol. 1989 Aug;23(3):257-62
references.pmid: 2787807
references.citation: Neurogastroenterol Motil. 1997 Dec;9(4):271-9
references.pmid: 9430796
references.citation: J Physiol. 2003 Dec 15;553(Pt 3):959-66
references.pmid: 14555722
references.citation: Gastroenterology. 2004 Mar;126(3):693-702
references.pmid: 14988823
references.citation: Am J Physiol. 1990 Mar;258(3 Pt 1):G329-37
references.pmid: 2316647
references.citation: Gastroenterology. 1988 Mar;94(3):611-21
references.pmid: 2828144
references.citation: J Gastroenterol Hepatol. 2006 Jan;21(1 Pt 1):71-8
references.pmid: 16706815
references.citation: Am J Physiol. 1987 Oct;253(4 Pt 1):G582-6
references.pmid: 2821826
references.citation: Nat Med. 2001 Jul;7(7):821-6
references.pmid: 11433347
references.citation: Urol Clin North Am. 1994 Feb;21(1):41-53
references.pmid: 8284844
references.citation: Gastroenterology. 1993 Mar;104(3):716-23
references.pmid: 8440432
references.citation: Proc Natl Acad Sci U S A. 1999 Dec 7;96(25):14629-34
references.pmid: 10588756
references.citation: Gastroenterology. 2003 Dec;125(6):1651-9
references.pmid: 14724817
references.citation: J Korean Med Sci. 2003 Apr;18(2):204-10
references.pmid: 12692417
references.citation: Aliment Pharmacol Ther. 2004 Dec;20(11-12):1317-22
references.pmid: 15606393
references.citation: Gastroenterology. 1988 Dec;95(6):1510-7
references.pmid: 2846402
references.citation: Gastroenterology. 1993 Jun;104(6):1692-9
references.pmid: 8500728