title: Impact of microcystin containing diets on physiological performance of Nile tilapia (Oreochromis niloticus) concerning stress and growth.
abstract: Diets containing Microcystis with considerable amounts of the cyanotoxin microcystin-LR (MC-LR) were fed to determine their impact on the physiological performance of the omnivorous Nile tilapia (Oreochromis niloticus) with regard to stress and growth performance. Four different diets were prepared based on a commercial diet (control, MC-5% [containing 5% dried Microcystis biomass], MC-20% [containing 20% dried Microcystis biomass], and Arthrospira-20% [containing 20% dried Arthrospira sp. biomass without toxin]) and fed to female Nile tilapia. Blood and tissue samples were taken after 1, 7, and 28 d, and MC-LR was quantified in gills, muscle, and liver by using high-performance liquid chromatography (HPLC). Only in the liver were moderate concentrations of MC-LR detected. The stress hormone cortisol and glucose were analyzed from plasma, suggesting that all modified diets caused only minor to moderate stress, which was confirmed by analyses of hepatic glycogen. In addition, the effects of the different diets on growth performance were investigated by determining gene expression of hypophyseal growth hormone (GH) and hepatic insulin-like growth factor-I (IGF-I). For all diets, quantitative reverse transcription-polymerase chain reaction (RT-qPCR) demonstrated no significant effect on gene expression of the major endocrine hormones of the growth axis, whereas classical growth data, including growth and feed conversion ratio, displayed slight inhibitory effects of all modified diets independent of their MC-LR content. However, no significant change was found in condition or hepatosomatic index among the various diets, so it seems feasible that dried cyanobacterial biomass might be even used as a component in fish diet for Nile tilapia, which requires further research in more detail.
authors.affiliation: Department of Fisheries and Hydrobiology, Mendel University of Agriculture and Forestry, Zemedelská 1, Brno, Czech Republic. andrea.zikova@seznam.cz
authors.forename: Andrea
authors.initials: A
authors.lastname: Ziková
authors.forename: Achim
authors.initials: A
authors.lastname: Trubiroha
authors.forename: Claudia
authors.initials: C
authors.lastname: Wiegand
authors.forename: Sven
authors.initials: S
authors.lastname: Wuertz
authors.forename: Bernhard
authors.initials: B
authors.lastname: Rennert
authors.forename: Stephan
authors.initials: S
authors.lastname: Pflugmacher
authors.forename: Radovan
authors.initials: R
authors.lastname: Kopp
authors.forename: Jan
authors.initials: J
authors.lastname: Mares
authors.forename: Werner
authors.initials: W
authors.lastname: Kloas
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D023681:Cichlids / Q000254:growth & development* / Q000378:metabolism; D004032:Diet; D005260:Female; D013006:Growth Hormone / Q000235:genetics; D006854:Hydrocortisone / Q000097:blood; D008112:Liver Glycogen / Q000032:analysis; D008387:Marine Toxins; D052998:Microcystins / Q000633:toxicity*; D012333:RNA, Messenger / Q000032:analysis; D014871:Water Microbiology
chemical_list: D001786:Blood Glucose; D008112:Liver Glycogen; D008387:Marine Toxins; D052998:Microcystins; D012333:RNA, Messenger; D013006:Growth Hormone; C057862:cyanoginosin LR; D006854:Hydrocortisone