Changes of collagen and nicotinamide adenine dinucleotide in human cancerous and normal prostate tissues studied using native fluorescence spectroscopy with selective excitation wavelength.
The fluorescence spectra of human cancerous and normal prostate tissues obtained by the selective excitation wavelength of 340 nm were measured. The contributions of principle biochemical components to tissue fluorescence spectra were investigated using the method of multivariate curve resolution with alternating least squares. The results show that there is a reduced contribution from the emission of collagen and increased contribution from nicotinamide adenine dinucleotide (NADH) in cancerous tissues as compared with normal tissue. This difference is attributed to the changes of relative contents of NADH and collagen during cancer development. This research may present a potential native biomarker for prostate cancer detection.
Pu
Yang
Y
City College of the City University of New York, Institute for Ultrafast Spectroscopy and Lasers, Department of Electrical Engineering, New York, New York 10031, USA.
Wang
Wubao
W
Wang
Wubao B
WB
Tang
Guichen
G
Tang
Guichen C
GC
Alfano
Robert R
RR
D014408:Biomarkers, Tumor / Q000032:analysis*; D003094:Collagen / Q000032:analysis*; D006801:Humans; D008297:Male; D009243:NAD / Q000032:analysis*; D011471:Prostatic Neoplasms / Q000175:diagnosis* / Q000378:metabolism*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*; D014018:Tissue Distribution
D014408:Biomarkers, Tumor; D009243:NAD; D003094:Collagen