title: Antihypertensive effect of insect cells: in vitro and in vivo evaluation.
abstract: In this study, we investigated the in vitro ACE inhibitory and in vivo antihypertensive effect of insect cell extracts. The IC(50) of three insect cell lines from different type and insect species origin: S2 (embryo, Drosophila melanogaster), Sf21 (ovary, Spodoptera frugiperda) and Bm5 (ovary, Bombyx mori), were evaluated. Most interesting results were that the IC(50) values ranged between 0.4 and 0.9 mg/ml, and that an extra hydrolysis with gastrointestinal enzymes did not increase the ACE inhibitory activity conspicuously. Finally, a single oral administration with a gavage of 150 mg cell extract/kg BW to spontaneous hypertensive rats (SHR) significantly decreased (p<0.05) their systolic blood pressure (SBP) with 5-6% (9-12 mmHg) compared to the controls at 6 h post-administration. Here the undigested and digested insect S2 cell extracts were equal in activity to lower the SBP. To the best of our knowledge, this is the first report of in vivo antihypertensive activity of insect cell extracts and this without an extra digestion requirement.
authors.affiliation: Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, B-9000 Ghent, Belgium.
authors.forename: Dorien
authors.initials: D
authors.lastname: Staljanssens
authors.forename: John
authors.initials: J
authors.lastname: Van Camp
authors.forename: Griet
authors.initials: G
authors.lastname: Herregods
authors.forename: Maarten
authors.initials: M
authors.lastname: Dhaenens
authors.forename: Dieter
authors.initials: D
authors.lastname: Deforce
authors.forename: Johan
authors.initials: J
authors.lastname: Van de Voorde
authors.forename: Guy
authors.initials: G
authors.lastname: Smagghe
mesh_terms: D000818:Animals; D000959:Antihypertensive Agents / Q000737:chemistry / Q000627:therapeutic use*; D001794:Blood Pressure; D012831:Bombyx / Q000737:chemistry; D002460:Cell Line; D002850:Chromatography, Gel; D004331:Drosophila melanogaster / Q000737:chemistry; D005260:Female; D006973:Hypertension / Q000188:drug therapy*; D020128:Inhibitory Concentration 50; D008297:Male; D051379:Mice; D051381:Rats; D011918:Rats, Inbred SHR; D018411:Spodoptera / Q000737:chemistry
chemical_list: D000959:Antihypertensive Agents