title: Packing of cholesterol molecules in human high-density lipoproteins.
abstract: High-resolution, proton-decoupled (13)C nuclear magnetic resonance spectra (90.55 MHz) of human high-density lipoproteins (HDL) have been employed to investigate the physical state of unesterified cholesterol molecules in such particles. The cholesterol molecules in HDL(2), and HDL(3) were replaced with [4-(13)C]cholesterol by either particle reconstitution or exchange from Celite. Two well-defined resonances from [4-(13)C]cholesterol molecules in HDL (2) and HDL(3) were observed at chemical shifts (delta) of 41.70 and 42.20 ppm, indicating that cholesterol molecules are present in two distinct environments. The signal at delta 41.70 arises from the C-4 atom of cholesterol molecules associated with the phospholipid monolayer at the surface of the particles. The resonance at delta 42.20 is due to the 4-(13)C atom of cholesterol molecules dissolved in the cholesterol ester/triglyceride core. Decomposition of the two [4-(13)C]cholesterol resonances shows that approximately 40% of the signal arises from molecules in the apolar core, with the remainder due to molecules in the surface. Spin-lattice relaxation time and line-width measurements indicate that the cholesterol molecules dissolved in the core are relatively disordered and mobile. The cholesterol molecules located among phospholipid molecules in the surface of the particle undergo relatively restricted, anisotropic motions. The chemical shifts and relaxation enhancements induced by the addition of paramagnetic ions to the aqueous phase indicate that the surface cholesterol molecules in HDL(2), and HDL(3) are exposed to the water and that the 4-(13)C atom of cholesterol is located in the region of the phospholipid acylcarboxyl groups.The NMR data indicate that the residence time for cholesterol molecules in either the surface or the core pools of HDL is greater than or equal to 10 ms. However, more than 90% of the unesterified cholesterol molecules in HDL are in a single kinetic pool for exchange with cholesterol molecules in other lipoprotein particles or cells. It follows that cholesterol molecules in the two microenvironments undergo fast exchange on the biological time scale and can equilibrate between the surface and core of HDL in the time scale 10 ms-ca. 300 s. Neither the surface nor the core microenvironments of human HDL particles are saturated with cholesterol.
authors.forename: S
authors.initials: S
authors.lastname: Lund-Katz
authors.forename: M C
authors.initials: MC
authors.lastname: Phillips
mesh_terms: D002784:Cholesterol / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D008075:Lipoproteins, HDL / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008345:Manganese / Q000737:chemistry; D008958:Models, Molecular
chemical_list: D008075:Lipoproteins, HDL; D008345:Manganese; D002784:Cholesterol