title: VIVID interacts with the WHITE COLLAR complex and FREQUENCY-interacting RNA helicase to alter light and clock responses in Neurospora.
abstract: The photoreceptor and PAS/LOV protein VIVID (VVD) modulates blue-light signaling and influences light and temperature responses of the circadian clock in Neurospora crassa. One of the main actions of VVD on the circadian clock is to influence circadian clock phase by regulating levels of the transcripts encoded by the central clock gene frequency (frq). How this regulation is achieved is unknown. Here we show that VVD interacts with complexes central for circadian clock and blue-light signaling, namely the WHITE-COLLAR complex (WCC) and FREQUENCY-interacting RNA helicase (FRH), a component that complexes with FRQ to mediate negative feedback control in Neurospora. VVD interacts with FRH in the absence of WCC and FRQ but does not seem to control the exosome-mediated negative feedback loop. Instead, VVD acts to modulate the transcriptional activity of the WCC.
authors.affiliation: Faculty of Life Sciences, University of Manchester, Manchester M13 9PT, United Kingdom.
authors.forename: Suzanne M
authors.initials: SM
authors.lastname: Hunt
authors.forename: Seona
authors.initials: S
authors.lastname: Thompson
authors.forename: Mark
authors.initials: M
authors.lastname: Elvin
authors.forename: Christian
authors.initials: C
authors.lastname: Heintzen
mesh_terms: D001483:Base Sequence; D002467:Cell Nucleus / Q000378:metabolism; D002940:Circadian Rhythm / Q000235:genetics / Q000502:physiology*; D003593:Cytoplasm / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000502:physiology*; D025461:Feedback, Physiological; D005656:Fungal Proteins / Q000235:genetics / Q000502:physiology*; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D008027:Light; D008954:Models, Biological; D009492:Neurospora crassa / Q000235:genetics / Q000502:physiology* / Q000528:radiation effects*; D020283:Photoreceptors, Microbial / Q000235:genetics / Q000502:physiology; D020365:RNA Helicases / Q000235:genetics / Q000502:physiology*; D012331:RNA, Fungal / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology*
chemical_list: D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D020283:Photoreceptors, Microbial; D012331:RNA, Fungal; D012333:RNA, Messenger; D014157:Transcription Factors; C423283:VVD protein, Neurospora crassa; C105589:wc-1 protein, Neurospora crassa; C103772:white collar 2 protein, Neurospora; D020365:RNA Helicases
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