title: The conserved RNA trafficking proteins HPR1 and TEX1 are involved in the production of endogenous and exogenous small interfering RNA in Arabidopsis.
abstract: We previously identified Arabidopsis thaliana mutants defective in sense transgene posttranscriptional gene silencing (S-PTGS) that defined six loci; here, we describe mutants that define nine additional loci, including HYPER RECOMBINATION1 (HPR1), SILENCING DEFECTIVE3 (SDE3), and SDE5. Our analyses extend previous findings by showing that the requirement for the putative RNA helicase SDE3 is inversely proportional to the strength of the PTGS inducer and that the putative RNA trafficking protein SDE5 is an essential component of the trans-acting small interfering RNA (tasiRNA) pathway and is required for S-PTGS but not inverted repeat transgene-mediated PTGS (IR-PTGS). Our screen also identified HPR1 as a PTGS actor. We show that hpr1 mutations negatively impact S-PTGS, IR-PTGS, and tasiRNA pathways, resulting in increased accumulation of siRNA precursors and decreased accumulation of mature siRNA. In animals, HPR1/THO1 is a member of the conserved RNA trafficking THO/TREX complex, which also includes TEX1/THO3. We show that tex1 mutants, like hpr1 mutants, impact TAS precursor and mature tasiRNA levels, suggesting that a THO/TREX complex exists in plants and that this complex is important for the integrity of the tasiRNA pathway. We propose that both HPR1 and TEX1 participate in the trafficking of siRNA precursors to the ARGONAUTE catalytic center.
authors.affiliation: Institut Jean-Pierre Bourgin, Institut National de la Recherche Agronomique, 78026 Versailles Cedex, France.
authors.forename: Vincent
authors.initials: V
authors.lastname: Jauvion
authors.forename: Taline
authors.initials: T
authors.lastname: Elmayan
authors.forename: Hervé
authors.initials: H
authors.lastname: Vaucheret
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D018744:DNA, Plant / Q000235:genetics; D016254:Mutagenesis, Insertional; D009154:Mutation; D021381:Protein Transport; D020365:RNA Helicases / Q000378:metabolism; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D018744:DNA, Plant; C552050:EMU protein, Arabidopsis; D034741:RNA, Small Interfering; C522029:SDE5 protein, Arabidopsis; C426019:SDE3 protein, Arabidopsis; D020365:RNA Helicases
references.citation: Curr Biol. 2005 Aug 23;15(16):1501-7
references.pmid: 16111943
references.citation: Curr Biol. 2006 May 9;16(9):927-32
references.pmid: 16682354
references.citation: Curr Biol. 2002 Apr 16;12(8):684-8
references.pmid: 11967158
references.citation: Science. 2005 Apr 1;308(5718):118-20
references.pmid: 15692015
references.citation: Science. 2006 Jul 7;313(5783):68-71
references.pmid: 16741077
references.citation: Proc Natl Acad Sci U S A. 2005 Aug 16;102(33):11928-33
references.pmid: 16081530
references.citation: Proc Natl Acad Sci U S A. 2010 Aug 3;107(31):13948-53
references.pmid: 20634427
references.citation: Plant Cell. 2002 Mar;14(3):629-39
references.pmid: 11910010
references.citation: Mol Cell. 2004 Jul 23;15(2):173-83
references.pmid: 15260969
references.citation: Nucleic Acids Res. 2010 Sep;38(17):5844-50
references.pmid: 20448024
references.citation: Trends Genet. 2008 Apr;24(4):151-4
references.pmid: 18325623
references.citation: Science. 2010 May 14;328(5980):872-5
references.pmid: 20413459
references.citation: Cell. 2009 Feb 20;136(4):669-87
references.pmid: 19239888
references.citation: Science. 2010 May 14;328(5980):912-6
references.pmid: 20413458
references.citation: Nucleic Acids Res. 2002 Jan 1;30(1):94-7
references.pmid: 11752264
references.citation: Science. 2005 Feb 11;307(5711):932-5
references.pmid: 15705854
references.citation: Proc Natl Acad Sci U S A. 2006 Oct 10;103(41):14994-5001
references.pmid: 17008405
references.citation: EMBO J. 1997 Aug 1;16(15):4738-45
references.pmid: 9303318
references.citation: Proc Natl Acad Sci U S A. 2000 Oct 10;97(21):11650-4
references.pmid: 11016954
references.citation: Plant Cell. 2007 May;19(5):1507-21
references.pmid: 17526749
references.citation: Science. 2004 Nov 5;306(5698):1046-8
references.pmid: 15528448
references.citation: Proc Natl Acad Sci U S A. 2007 Sep 11;104(37):14741-6
references.pmid: 17785412
references.citation: Dev Cell. 2008 Jun;14(6):854-66
references.pmid: 18486559
references.citation: Cell. 2000 May 26;101(5):533-42
references.pmid: 10850495
references.citation: Science. 2003 Aug 1;301(5633):653-7
references.pmid: 12893945
references.citation: Nucleic Acids Res. 2006;34(21):6233-46
references.pmid: 17090584
references.citation: EMBO Rep. 2009 May;10(5):521-6
references.pmid: 19343050
references.citation: EMBO Rep. 2006 Nov;7(11):1168-75
references.pmid: 17039251
references.citation: Genes Dev. 2004 Oct 1;18(19):2368-79
references.pmid: 15466488
references.citation: EMBO J. 2006 Jul 26;25(14):3347-56
references.pmid: 16810317
references.citation: Proc Natl Acad Sci U S A. 2010 Jan 5;107(1):466-71
references.pmid: 20018656
references.citation: Proc Natl Acad Sci U S A. 2009 May 26;106(21):8772-7
references.pmid: 19439664
references.citation: Curr Biol. 2000 Dec 14-28;10(24):1591-4
references.pmid: 11137011
references.citation: Nat Genet. 2006 Jun;38 Suppl:S31-6
references.pmid: 16736022
references.citation: Proc Natl Acad Sci U S A. 2008 Jun 24;105(25):8795-800
references.pmid: 18550839
references.citation: EMBO J. 2010 May 19;29(10):1699-712
references.pmid: 20414198
references.citation: Mol Cell. 2004 Oct 8;16(1):69-79
references.pmid: 15469823
references.citation: Genes Dev. 2005 Sep 15;19(18):2164-75
references.pmid: 16131612
references.citation: Cell. 1998 Oct 16;95(2):177-87
references.pmid: 9790525
references.citation: Cell. 2005 Apr 22;121(2):207-21
references.pmid: 15851028
references.citation: PLoS One. 2008 Mar 12;3(3):e1755
references.pmid: 18335032
references.citation: PLoS Genet. 2009 Sep;5(9):e1000646
references.pmid: 19763164
references.citation: Nat Genet. 2005 Dec;37(12):1356-60
references.pmid: 16273107
references.citation: Trends Genet. 2008 Oct;24(10):511-7
references.pmid: 18778867
references.citation: Plant Mol Biol. 2005 Jan;57(2):173-88
references.pmid: 15821876
references.citation: Science. 2008 May 30;320(5880):1185-90
references.pmid: 18483398
references.citation: Nat Genet. 2007 Jul;39(7):848-56
references.pmid: 17558406
references.citation: Plant Cell. 1998 Oct;10(10):1747-58
references.pmid: 9761800
references.citation: Science. 2007 Oct 5;318(5847):109-12
references.pmid: 17916737
references.citation: Curr Biol. 2003 May 13;13(10):843-8
references.pmid: 12747833
references.citation: Proc Natl Acad Sci U S A. 2008 Jan 8;105(1):157-61
references.pmid: 18165314
references.citation: Plant Cell. 2007 Mar;19(3):943-58
references.pmid: 17384170
references.citation: Plant J. 2007 Apr;50(1):140-8
references.pmid: 17397509
references.citation: Cell. 2007 Aug 10;130(3):413-26
references.pmid: 17693253
references.citation: Cell. 2000 May 26;101(5):543-53
references.pmid: 10850496
references.citation: EMBO J. 2001 Apr 17;20(8):2069-78
references.pmid: 11296239
references.citation: FEBS J. 2009 Feb;276(3):835-44
references.pmid: 19143842
references.citation: Curr Opin Cell Biol. 2005 Jun;17(3):269-73
references.pmid: 15901496
references.citation: Nature. 2000 Sep 21;407(6802):319-20
references.pmid: 11014180
references.citation: Nucleic Acids Res. 2004 Mar 19;32(5):1792-7
references.pmid: 15034147
references.citation: FEBS Lett. 2009 Apr 17;583(8):1261-6
references.pmid: 19332064
references.citation: Plant Cell. 2007 Nov;19(11):3451-61
references.pmid: 17993620