Heterologous production of pediocin PA-1 in Lactobacillus reuteri.
The recombinant DNA pLR5cat_PSAB in which pediocin PA-1 structural and immunity genes (pedAB) fused with the promoter and deduced signal sequence of an alpha-amylase gene from a bifidobacterial strain were inserted in pLR5cat, an Escherichia coli-lactobacilli shuttle vector was transferred to Lactobacillus reuteri KCTC 3679 and the transformant presented bacteriocin activity. The recombinant L. reuteri KCTC 3679 transformed with the shortened pLR5cat(S)_PSAB, where non-essential region for the lactobacilli replicon was removed, also showed bacteriocin activity. The molecular mass of the secreted pediocin PA-1 from the recombinant bacteria was the same as that of native pediocin PA-1 (~4.6 kDa) from Pediococcus acidilactici K10 on a sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gel. In co-cultures with Listeria monocytogenes, the recombinant L. reuteri KCTC 3679 effectively reduced the viable cell count of the pathogenic bacterium by a 3 log scale compared with a control where L. monocytogenes was incubated alone.
Eom
Ji-Eun
JE
Department of Biotechnology, Chungju National University, Jeungpyeong, Korea.
Moon
Sung-Kwon
SK
Moon
Gi-Seong
GS
D000900:Anti-Bacterial Agents / Q000378:metabolism* / Q000494:pharmacology; D001430:Bacteriocins / Q000235:genetics* / Q000378:metabolism* / Q000494:pharmacology; D015870:Gene Expression; D052200:Limosilactobacillus reuteri / Q000235:genetics* / Q000378:metabolism; D008089:Listeria monocytogenes / Q000187:drug effects / Q000254:growth & development; D000070017:Pediocins; D015202:Protein Engineering; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D001430:Bacteriocins; D000070017:Pediocins; D011993:Recombinant Fusion Proteins; C074288:pediocin PA-1