title: Analysis and difference of voltage-dependent anion channel mRNA in ejaculated spermatozoa from normozoospermic fertile donors and infertile patients with idiopathic asthenozoospermia.
abstract: OBJECTIVE
  to analyze the abundance and difference of voltage-dependent anion channel (VDAC) mRNA in ejaculated spermatozoa from normozoospermic fertile donors and infertile patients with idiopathic asthenozoospermia.
  
  
  METHODS
  high motile and low motile spermatozoa were separated respectively from ejaculates of 36 donors and 40 patients using a discontinuous Percoll gradient centrifugation. Real-Time PCR was performed to detect mRNA abundance and difference of three VDAC subtypes between two groups with different sperm motility.
  
  
  RESULTS
  real-Time PCR demonstrated that three VDAC mRNAs were present in mature spermatozoa. The VDAC2 mRNA level in ejaculated spermatozoa of patients was significantly higher than that of donors. No significant differences of VDAC1 and VDAC3 mRNA levels were found between two groups.
  
  
  CONCLUSIONS
  the high abundance of VDAC2 mRNA seemed to have a positive correlation with low sperm motility. The abnormal expression of VDAC might be related to male infertility with idiopathic asthenozoospermia.
authors.affiliation: Laboratory of Reproductive Medicine, Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
authors.forename: Bianjiang
authors.initials: B
authors.lastname: Liu
authors.forename: Peng
authors.initials: P
authors.lastname: Wang
authors.forename: Zengjun
authors.initials: Z
authors.lastname: Wang
authors.forename: Yuejun
authors.initials: Y
authors.lastname: Jia
authors.forename: Xiaobing
authors.initials: X
authors.lastname: Niu
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
mesh_terms: D000328:Adult; D053627:Asthenozoospermia / Q000235:genetics*; D016022:Case-Control Studies; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism; D013081:Sperm Motility; D013094:Spermatozoa / Q000378:metabolism*; D050996:Voltage-Dependent Anion Channel 2 / Q000378:metabolism*; D055815:Young Adult
chemical_list: D012333:RNA, Messenger; C494765:VDAC2 protein, human; D050996:Voltage-Dependent Anion Channel 2
references.citation: Biochem Biophys Res Commun. 2006 Aug 4;346(3):665-70
references.pmid: 16774740
references.citation: Int J Dev Biol. 2008;52(5-6):463-72
references.pmid: 18649259
references.citation: FEBS Lett. 1996 May 20;386(2-3):205-10
references.pmid: 8647283
references.citation: Biol Reprod. 2005 Jan;72(1):22-32
references.pmid: 15342355
references.citation: Proc Natl Acad Sci U S A. 2004 Nov 23;101(47):16501-6
references.pmid: 15546993
references.citation: Cell Motil Cytoskeleton. 2006 Feb;63(2):66-76
references.pmid: 16374831
references.citation: J Biol Chem. 2003 Jul 18;278(29):27312-8
references.pmid: 12736244
references.citation: Mamm Genome. 1999 Oct;10(10):1041-2
references.pmid: 10501981
references.citation: Biosci Rep. 2009 Jul 22;29(6):351-62
references.pmid: 18976238
references.citation: Pflugers Arch. 2008 Jan;455(4):677-86
references.pmid: 17647012
references.citation: J Membr Biol. 1976 Dec 28;30(2):99-120
references.pmid: 1011248
references.citation: J Mol Endocrinol. 2004 Feb;32(1):279-89
references.pmid: 14766008
references.citation: Biol Reprod. 2003 May;68(5):1590-6
references.pmid: 12606347
references.citation: Biochimie. 2002 Feb-Mar;84(2-3):187-93
references.pmid: 12022949
references.citation: Nature. 2004 May 13;429(6988):154
references.pmid: 15141202
references.citation: Arch Androl. 2003 Sep-Oct;49(5):343-9
references.pmid: 12893510
references.citation: J Mol Med (Berl). 2004 May;82(5):317-24
references.pmid: 14985855
references.citation: Lancet. 2002 Sep 7;360(9335):772-7
references.pmid: 12241836
references.citation: Biochim Biophys Acta. 2001 Apr 16;1518(3):329-33
references.pmid: 11311949
references.citation: J Membr Biol. 1997 Jun 1;157(3):271-9
references.pmid: 9178614
references.citation: Mol Hum Reprod. 2004 Jul;10(7):535-41
references.pmid: 15100385
references.citation: Biochem Biophys Res Commun. 2009 Jan 16;378(3):366-70
references.pmid: 19013129
references.citation: J Biol Chem. 2006 Jan 27;281(4):1897-904
references.pmid: 16291750
references.citation: Biochem Biophys Res Commun. 2010 Jul 9;397(4):633-6
references.pmid: 20541530
references.citation: BMC Evol Biol. 2007 Feb 28;7:31
references.pmid: 17328803
references.citation: Science. 2003 Jul 25;301(5632):513-7
references.pmid: 12881569
references.citation: Biophys J. 1998 May;74(5):2365-73
references.pmid: 9591663
references.citation: Biochim Biophys Acta. 2005 Dec 20;1710(2-3):96-102
references.pmid: 16293222
references.citation: Mol Genet Metab. 2000 May;70(1):69-74
references.pmid: 10833333
references.citation: J Assist Reprod Genet. 2010 Feb;27(2-3):111-20
references.pmid: 20127162
references.citation: Mol Aspects Med. 2010 Jun;31(3):227-85
references.pmid: 20346371
references.citation: J Mol Biol. 2010 Feb 26;396(3):580-92
references.pmid: 20005234
references.citation: Biochim Biophys Acta. 1994 Jun 29;1197(2):167-96
references.pmid: 8031826
references.citation: Biochem Biophys Res Commun. 1999 Feb 24;255(3):707-10
references.pmid: 10049775
references.citation: Mol Hum Reprod. 2003 Mar;9(3):117-24
references.pmid: 12606587
references.citation: J Biol Chem. 2001 Oct 19;276(42):39206-12
references.pmid: 11507092
references.citation: Biol Chem Hoppe Seyler. 1994 Aug;375(8):513-20
references.pmid: 7529026
references.citation: J Biol Chem. 2004 Apr 9;279(15):15281-8
references.pmid: 14739283