title: Chromosome abnormality rates in human embryos obtained from in-vitro maturation and IVF treatment cycles.
abstract: The aim of this retrospective study was to compare the incidence of chromosomal abnormality in embryos from in-vitro maturation (IVM) and IVF cycles. The copy numbers of chromosomes 13, 15, 16, 18, 21, 22, X and Y were assessed with fluorescence in-situ hybridization (FISH) in single blastomeres biopsied from cleavage stage embryos. Spare embryos that were not transferred or cryopreserved were also analysed in full. IVM and IVF groups comprised six and 30 couples, with mean ± SD embryos with FISH result of 8.0 ± 4.4 and 11.7 ± 3.8, respectively. The incidence of chromosomal abnormality per FISH result was similar in IVM and IVF embryos (58.7% versus 57.4%, respectively). When embryos were categorized based on maturation time of oocytes in IVM cycles, embryos derived from oocytes that matured 48 h after collection had a higher chromosomal abnormality rate compared with embryos derived from in-vivo matured oocytes and to embryos derived from oocytes that matured in the first 24 h after collection.
authors.affiliation: McGill Reproductive Centre, Department of Obstetrics and Gynecology, Royal Victoria Hospital, McGill University Health Center, Montreal, Quebec, Canada.
authors.forename: Xiao Yun
authors.initials: XY
authors.lastname: Zhang
authors.forename: Baris
authors.initials: B
authors.lastname: Ata
authors.forename: Weon-Young
authors.initials: WY
authors.lastname: Son
authors.forename: William M
authors.initials: WM
authors.lastname: Buckett
authors.forename: Seang-Lin
authors.initials: SL
authors.lastname: Tan
authors.forename: Asangla
authors.initials: A
authors.lastname: Ao
mesh_terms: D000328:Adult; D000782:Aneuploidy; D001757:Blastomeres / Q000648:ultrastructure; D002869:Chromosome Aberrations / Q000706:statistics & numerical data*; D046149:Embryo Culture Techniques; D047108:Embryonic Development; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D009865:Oocytes; D019836:Preimplantation Diagnosis; D012189:Retrospective Studies