title: In vivo cellular MRI of dendritic cell migration using micrometer-sized iron oxide (MPIO) particles.
abstract: OBJECTIVE
  This study seeks to assess the use of labeling with micron-sized iron oxide (MPIO) particles for the detection and quantification of the migration of dendritic cells (DCs) using cellular magnetic resonance imaging (MRI).
  
  
  METHODS
  DCs were labeled with red fluorescent MPIO particles for detection by cellular MRI and a green fluorescent membrane dye (PKH67) for histological detection. MPIO-labeled DCs or unlabeled control DCs were injected into mice footpads at two doses (0.1 × 10(6) and 1 × 10(6)). Images were acquired at 3 Tesla before DC injection and 2, 3, and 7 days post-DC injection.
  
  
  RESULTS
  Labeling DCs with MPIO particles did not affect viability, but it did alter markers of DC activation and maturation. MRI and fluorescence microscopy allowed for the detection of MPIO-labeled DCs within the draining popliteal nodes after their injection into the footpad.
  
  
  CONCLUSIONS
  This paper presents the first report of the successful use of fluorescent MPIO particles to label and track DC migration.
authors.affiliation: Department of Medical Biophysics, University of Western Ontario, London, Ontario, Canada.
authors.forename: Roja
authors.initials: R
authors.lastname: Rohani
authors.forename: Sonali N
authors.initials: SN
authors.lastname: de Chickera
authors.forename: Christy
authors.initials: C
authors.lastname: Willert
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Chen
authors.forename: Gregory A
authors.initials: GA
authors.lastname: Dekaban
authors.forename: Paula J
authors.initials: PJ
authors.lastname: Foster
mesh_terms: D000818:Animals; D017209:Apoptosis; D001854:Bone Marrow Cells / Q000166:cytology; D002465:Cell Movement; D002470:Cell Survival; D003713:Dendritic Cells / Q000166:cytology* / Q000378:metabolism; D005290:Ferric Compounds / Q000737:chemistry* / Q000378:metabolism*; D005453:Fluorescence; D008198:Lymph Nodes / Q000473:pathology; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009930:Organic Chemicals / Q000378:metabolism; D010316:Particle Size; D010641:Phenotype; D013194:Staining and Labeling
chemical_list: D005290:Ferric Compounds; D009930:Organic Chemicals; C451241:PKH67; C000499:ferric oxide
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