title: The conserved CXXC motif of hepatic stimulator substance is essential for its role in mitochondrial protection in H2O2-induced cell apoptosis.
abstract: Hepatic stimulator substance (HSS) protects liver cells from various toxins by alleviating lesions caused in the mitochondria. This paper demonstrates the necessity of the conserved CXXC catalytic motif (C62-C65) for the mitochondria-targeted anti-apoptotic activity of HSS. Mutating the conserved CXXC motif eliminated the protective effects against H(2)O(2)-induced apoptosis and diminished the protection of the mitochondria. However, the mutation of the other disulfide bond C91-C108 mainly preserved the protection of mitochondria by HSS, implying that the conserved CXXC motif and sulfhydryl oxidase (SOX) activity are essential for mitochondrial protection.
authors.affiliation: Department of Cell Biology and Municipal Laboratory for Liver Protection and Regulation of Regeneration, Capital Medical University, Beijing, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Li
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: An
mesh_terms: D020816:Amino Acid Motifs; D017209:Apoptosis; D002384:Catalysis; D045744:Cell Line, Tumor; D017124:Conserved Sequence; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity; D036341:Intercellular Signaling Peptides and Proteins; D008099:Liver / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology; D008930:Mitochondria, Liver / Q000201:enzymology*; D009154:Mutation; D010455:Peptides / Q000235:genetics / Q000378:metabolism*
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; C073456:hepatic stimulator substance; D006861:Hydrogen Peroxide