title: Dynamic analysis of CD127 expression on memory CD8 T cells from patients with chronic hepatitis B during telbivudine treatment.
abstract: BACKGROUND
  Accumulating evidence supports the theory that expression of CD127 on CD8 T cells during the process of antiviral immune response indicates a subset of effect CD8 T cells that successfully develop into fully protective memory. CD8 T cells expression of CD127 may be used as a predictor to evaluate disease status in chronic viral infection. The aim of this study was to investigate the CD127 expression level on different subsets of CD8 T cell and explore the relationship between CD127 expression on CD8 memory T cells and serum hepatitis B virus (HBV) DNA and hepatitis B e antigen (HBeAg) levels in patients with chronic hepatitis B (CHB). We also aimed to investigate the CD127 expression pattern on CD8 memory T cells of CHB patients who were treated with Telbivudine.
  
  
  RESULTS
  Twenty HBeAg-positive CHB patients were selected and treated with telbivudine 600 mg/day for 48 weeks. The memory CD8 T cells were characterized by expression of CD45RA and CD27 markers. CD127 expression on the CD8 T-cell surface was measured by four-colour flow cytometry. Our results showed that CD127 expression on memory CD8 T cells was reduced in CHB patients. There was a strong negative correlation between CD127 expression on memory CD8 T cells and serum HBV DNA and HBeAg levels in CHB patients. Moreover, successful antiviral therapy increased CD127 expression on CD8 memory T cells as well as on HBV-specific CD8 T cells in CHB patients.
  
  
  CONCLUSIONS
  These results suggest that diminished CD127 expression on CD8 memory T cells of CHB patients is a potential mechanism explaining cellular immune function impairment in CHB infection, and that CD127 expression on CD8 memory T cells is a useful indicator for evaluating the effects of anti-HBV therapy.
authors.affiliation: State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Department of Infectious Diseases, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, P R of China.
authors.forename: Guocai
authors.initials: G
authors.lastname: Lv
authors.forename: Linjung
authors.initials: L
authors.lastname: Ying
authors.forename: Wen-Jiang
authors.initials: WJ
authors.lastname: Ma
authors.forename: Xi
authors.initials: X
authors.lastname: Jin
authors.forename: Lin
authors.initials: L
authors.lastname: Zheng
authors.forename: Lanjuan
authors.initials: L
authors.lastname: Li
authors.forename: Yida
authors.initials: Y
authors.lastname: Yang
mesh_terms: D000328:Adult; D000998:Antiviral Agents / Q000008:administration & dosage*; D018414:CD8-Positive T-Lymphocytes / Q000737:chemistry* / Q000276:immunology*; D004279:DNA, Viral / Q000097:blood; D005260:Female; D020869:Gene Expression Profiling; D006513:Hepatitis B e Antigens / Q000097:blood; D006515:Hepatitis B virus / Q000302:isolation & purification; D019694:Hepatitis B, Chronic / Q000188:drug therapy / Q000276:immunology*; D006801:Humans; D007156:Immunologic Memory; D053699:Interleukin-7 Receptor alpha Subunit / Q000032:analysis*; D008297:Male; D009705:Nucleosides / Q000008:administration & dosage*; D011744:Pyrimidinones / Q000008:administration & dosage*; D016176:T-Lymphocyte Subsets / Q000737:chemistry / Q000276:immunology; D000077712:Telbivudine; D013936:Thymidine / Q000031:analogs & derivatives
chemical_list: D000998:Antiviral Agents; D004279:DNA, Viral; D006513:Hepatitis B e Antigens; D053699:Interleukin-7 Receptor alpha Subunit; D009705:Nucleosides; D011744:Pyrimidinones; D000077712:Telbivudine; D013936:Thymidine
references.citation: J Acquir Immune Defic Syndr. 2001 Dec 15;28(5):454-7
references.pmid: 11744834
references.citation: PLoS One. 2008;3(12):e3986
references.pmid: 19096522
references.citation: J Virol. 2006 Apr;80(7):3532-40
references.pmid: 16537621
references.citation: Immunity. 2007 Sep;27(3):393-405
references.pmid: 17892848
references.citation: Lancet. 2003 Dec 20;362(9401):2089-94
references.pmid: 14697813
references.citation: J Immunol. 2005 May 15;174(10):5926-30
references.pmid: 15879083
references.citation: Clin Exp Immunol. 2006 Mar;143(3):398-403
references.pmid: 16487237
references.citation: Blood. 2005 Sep 15;106(6):2091-8
references.pmid: 15947093
references.citation: J Hepatol. 2002 Jan;36(1):105-15
references.pmid: 11804672
references.citation: Virology. 2006 Sep 30;353(2):366-73
references.pmid: 16860834
references.citation: Hepatology. 2008 Sep;48(3):759-69
references.pmid: 18697210
references.citation: J Virol. 2008 Oct;82(20):10017-31
references.pmid: 18667516
references.citation: Annu Rev Immunol. 2004;22:745-63
references.pmid: 15032595
references.citation: J Virol. 2004 Jun;78(11):5707-19
references.pmid: 15140968
references.citation: J Immunol. 2005 Mar 1;174(5):2900-9
references.pmid: 15728501
references.citation: Nat Immunol. 2003 Dec;4(12):1191-8
references.pmid: 14625547
references.citation: Nat Immunol. 2003 Sep;4(9):835-42
references.pmid: 12942084
references.citation: Antiviral Res. 2003 Oct;60(2):61-6
references.pmid: 14638399