Maternal high-fat intake predisposes nonalcoholic fatty liver disease in C57BL/6 offspring.
OBJECTIVE
This work aimed to verify the hypothesis that maternal intake of high-fat diet in critical periods of pregnancy and/or suckling period predisposes nonalcoholic fatty liver disease in adult C57BL/6 mice offspring.


METHODS
Male pups were divided into 5 groups: (1) SC, from standard chow-fed dams; (2) G, from high-fat chow (HF)-fed dams during the gestation (G) period; (3) L, from HF-fed dams during the lactation (L) period; (4) GL, from HF-fed dams during the gestation and lactation (GL) periods; and (5) GL/HF, from HF-fed dams during GL, maintaining an HF diet from postweaning to adulthood. We analyzed body mass, plasma blood, and liver structure.


RESULTS
The G offspring showed insulin resistance and lower glucose transporter-2 expression. Hepatic steatosis was present in the G, L, GL, and mainly in GL/HF offspring. Sterol regulatory element-binding protein-1c expression was higher in G, GL, and GL/HF offspring.


CONCLUSIONS
Programming by HF chow predisposes hepatic adverse remodeling in the liver of adult offspring.
Gregorio
Bianca M
BM
Institute of Biology, Laboratory of Morphometry and Cardiovascular Morphology, State University of Rio de Janeiro, Biomedical Center, Rio de Janeiro, Brazil.
Souza-Mello
Vanessa
V
Carvalho
Jorge J
JJ
Mandarim-de-Lacerda
Carlos A
CA
Aguila
Marcia B
MB
D000410:Alanine Transaminase / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D000704:Analysis of Variance; D000818:Animals; D001786:Blood Glucose; D015153:Blotting, Western; D001835:Body Weight; D004041:Dietary Fats / Q000378:metabolism*; D004198:Disease Susceptibility / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005234:Fatty Liver / Q000209:etiology* / Q000378:metabolism; D005260:Female; D005951:Glucose Tolerance Test; D007328:Insulin / Q000097:blood; D007333:Insulin Resistance; D008099:Liver / Q000378:metabolism*; D008297:Male; D039382:Maternal Nutritional Physiological Phenomena / Q000502:physiology*; D051379:Mice; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects / Q000378:metabolism*; D011863:Radioimmunoassay; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D001786:Blood Glucose; D004041:Dietary Fats; D007328:Insulin; D014409:Tumor Necrosis Factor-alpha; D000410:Alanine Transaminase; D000469:Alkaline Phosphatase