title: Evaluation of clumping factor A binding region A in a subunit vaccine against Staphylococcus aureus-induced mastitis in mice.
abstract: The present study evaluated the potential of recombinant binding region A of clumping factor A (rClfA-A) to be an effective component of a vaccine against mastitis induced by Staphylococcus aureus in the mouse. rClfA-A and inactivated S. aureus were each emulsified in Freund's adjuvant, mineral oil adjuvant, and Seppic adjuvant; phosphate-buffered saline was used as a control. Seven groups of 12 mice each were immunized intraperitoneally three times at 2-week intervals. The titers of IgG and subtypes thereof (IgG1 and IgG2a) in the rClfA-A-immunized group were more than 1,000-fold higher than those in the killed-bacteria-immunized group (P < 0.01). Of the three adjuvants used, mineral oil adjuvant induced the highest antibody levels for both antigens (P < 0.001). Furthermore, the anti-rClfA-A antibody capacities for bacterial adhesion and opsonizing phagocytosis were significantly greater in the rClfA-A-immunized group than in the killed-bacteria-immunized group (P < 0.05). Lactating mice immunized with either rClfA-A or inactivated vaccine were challenged with S. aureus via the intramammary route. The numbers of bacteria recovered from the murine mammary glands 24 h after inoculation were significantly lower in the rClfA-A group than in the killed-bacteria-immunized group (P < 0.001). Histologic examination of the mammary glands showed that rClfA-A immunization effectively preserved tissue integrity. Thus, rClfA-A emulsified in an oil adjuvant provides strong immune protection against S. aureus-induced mastitis in the mouse.
authors.affiliation: National Key Laboratory of Agricultural Microbiology, Wuhan 430070, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Gong
authors.forename: Changmin
authors.initials: C
authors.lastname: Hu
authors.forename: Haiyang
authors.initials: H
authors.lastname: Xu
authors.forename: Aizhen
authors.initials: A
authors.lastname: Guo
authors.forename: Huanchun
authors.initials: H
authors.lastname: Chen
authors.forename: Guangzhi
authors.initials: G
authors.lastname: Zhang
authors.forename: Lei
authors.initials: L
authors.lastname: Shi
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001422:Bacterial Adhesion / Q000276:immunology; D058491:Bacterial Load; D003030:Coagulase / Q000008:administration & dosage / Q000276:immunology*; D004195:Disease Models, Animal; D005260:Female; D005620:Freund's Adjuvant / Q000008:administration & dosage; D006651:Histocytochemistry; D007074:Immunoglobulin G / Q000097:blood; D007274:Injections, Intraperitoneal; D008297:Male; D008321:Mammary Glands, Animal / Q000382:microbiology / Q000473:pathology; D008413:Mastitis / Q000276:immunology / Q000517:prevention & control*; D051379:Mice; D008807:Mice, Inbred BALB C; D008853:Microscopy; D009895:Opsonin Proteins / Q000097:blood; D010587:Phagocytosis / Q000276:immunology; D013209:Staphylococcal Vaccines / Q000008:administration & dosage / Q000276:immunology*; D022223:Vaccines, Subunit / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; C515944:ClfA protein, Staphylococcus aureus; D003030:Coagulase; D007074:Immunoglobulin G; D009895:Opsonin Proteins; D013209:Staphylococcal Vaccines; D022223:Vaccines, Subunit; D005620:Freund's Adjuvant
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