Synthesis of a 2-selenothymidine phosphoramidite and its incorporation into oligodeoxyribonucleotides.
The detailed synthetic protocol for a 2-selenothymidine phosphoramidite and its use in preparing Se-derivatized oligonucleotides are described here. The Se-modified phosphoramidite synthesis was achieved by activating a 2-thiothymidine derivative, followed by introduction of selenium functionality. The coupling reaction yield of the 2-selenothymidine phosphoramidite during solid-phase synthesis is high (>95%), and the oligonucleotides containing the 2-selenothymidine derivatization are stable.
Zhang
Wen
W
Georgia State University, Atlanta, Georgia, USA.
Huang
Zheng
Z
D002851:Chromatography, High Pressure Liquid; D018360:Crystallography, X-Ray; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D009943:Organophosphorus Compounds / Q000138:chemical synthesis* / Q000737:chemistry*; D012643:Selenium / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013936:Thymidine / Q000737:chemistry*
D009838:Oligodeoxyribonucleotides; D009943:Organophosphorus Compounds; C434331:phosphoramidite; D012643:Selenium; D013936:Thymidine