title: DAY NEUTRAL FLOWERING does not act through GIGANTEA and FKF1 to regulate CONSTANS expression and flowering time.
abstract: The regulation of CONSTANS (CO) gene expression and protein levels is the critical factor in determining a plant's response to photoperiod, flowering is induced when high levels of CO protein are present in the light. The regulation of CO transcription is mediated in part by GIGANTEA (GI), FKF1 and the CYCLING DOF FACTORS (CDFs) and factors affecting the levels of these proteins will also affect CO expression. The DAY NEUTRAL FLOWERING (DNF) protein is an E3 ligase involved in repressing CO expression in the early part of the day. In this article we present evidence to support the argument that DNF is not acting through the GI/FKF1/CDF regulatory mechanism to repress CO expression, but that it acts on another transcriptional activator of CO.
authors.affiliation: Warwick HRI, Warwick University, Wellesbourne, Warwick, UK.
authors.forename: Karl
authors.initials: K
authors.lastname: Morris
authors.forename: Stephen P
authors.initials: SP
authors.lastname: Jackson
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D035264:Flowers / Q000254:growth & development*; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D008027:Light; D017440:Photoperiod; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D044767:Ubiquitin-Protein Ligases / Q000235:genetics / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; C092776:CONSTANS protein, Arabidopsis; D004268:DNA-Binding Proteins; C410504:FKF1 protein, Arabidopsis; C121183:GI protein, Arabidopsis; D014157:Transcription Factors; C568224:DNF protein, Arabidopsis; D044767:Ubiquitin-Protein Ligases
references.citation: Science. 2007 Oct 12;318(5848):261-5
references.pmid: 17872410
references.citation: Nature. 2001 Apr 26;410(6832):1116-20
references.pmid: 11323677
references.citation: FEBS Lett. 2006 Feb 20;580(5):1193-7
references.pmid: 16457821
references.citation: Plant Cell. 2005 Aug;17(8):2255-70
references.pmid: 16006578
references.citation: Plant Cell. 2010 Apr;22(4):1118-28
references.pmid: 20435904
references.citation: Nature. 2003 Nov 20;426(6964):302-6
references.pmid: 14628054
references.citation: Dev Cell. 2009 Jul;17(1):75-86
references.pmid: 19619493