title: Chondroitin sulfate N-acetylgalactosaminyltransferase-1 is required for normal cartilage development.
abstract: CS (chondroitin sulfate) is a glycosaminoglycan species that is widely distributed in the extracellular matrix. To understand the physiological roles of enzymes involved in CS synthesis, we produced CSGalNAcT1 (CS N-acetylgalactosaminyltransferase 1)-null mice. CS production was reduced by approximately half in CSGalNAcT1-null mice, and the amount of short-chain CS was also reduced. Moreover, the cartilage of the null mice was significantly smaller than that of wild-type mice. Additionally, type-II collagen fibres in developing cartilage were abnormally aggregated and disarranged in the homozygous mutant mice. These results suggest that CSGalNAcT1 is required for normal CS production in developing cartilage.
authors.affiliation: Niigata University, Chuo-ku, Japan.
authors.forename: Yumi
authors.initials: Y
authors.lastname: Watanabe
authors.forename: Kosei
authors.initials: K
authors.lastname: Takeuchi
authors.forename: Susumu
authors.initials: S
authors.lastname: Higa Onaga
authors.forename: Michiko
authors.initials: M
authors.lastname: Sato
authors.forename: Mika
authors.initials: M
authors.lastname: Tsujita
authors.forename: Manabu
authors.initials: M
authors.lastname: Abe
authors.forename: Rie
authors.initials: R
authors.lastname: Natsume
authors.forename: Minqi
authors.initials: M
authors.lastname: Li
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Furuichi
authors.forename: Mika
authors.initials: M
authors.lastname: Saeki
authors.forename: Tomomi
authors.initials: T
authors.lastname: Izumikawa
authors.forename: Ayumi
authors.initials: A
authors.lastname: Hasegawa
authors.forename: Minesuke
authors.initials: M
authors.lastname: Yokoyama
authors.forename: Shiro
authors.initials: S
authors.lastname: Ikegawa
authors.forename: Kenji
authors.initials: K
authors.lastname: Sakimura
authors.forename: Norio
authors.initials: N
authors.lastname: Amizuka
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kitagawa
authors.forename: Michihiro
authors.initials: M
authors.lastname: Igarashi
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D049109:Cell Proliferation; D019902:Chondrocytes / Q000166:cytology / Q000201:enzymology / Q000378:metabolism; D020219:Chondrogenesis; D002809:Chondroitin Sulfates / Q000096:biosynthesis*; D024043:Collagen Type II / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D006132:Growth Plate / Q000196:embryology / Q000201:enzymology* / Q000378:metabolism; D007150:Immunohistochemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D017350:N-Acetylgalactosaminyltransferases / Q000235:genetics / Q000378:metabolism*; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D024043:Collagen Type II; D018809:Proliferating Cell Nuclear Antigen; D002809:Chondroitin Sulfates; D017350:N-Acetylgalactosaminyltransferases; C569943:chondroitin N-acetylgalactosaminyltransferase-1, mouse; C472559:chondroitin sulfate N-acetylgalactosaminyltransferase-1
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