title: Gilgamesh is required for rutabaga-independent olfactory learning in Drosophila.
abstract: Cyclic AMP signaling in Drosophila mushroom body neurons, anchored by the adenylyl cyclase encoded by the rutabaga gene, is indispensable for olfactory memory formation. From a screen for new memory mutants, we identified alleles of the gilgamesh (gish) gene, which encodes a casein kinase Iγ homolog that is preferentially expressed in the mushroom body neurons. The gish-encoded kinase participates in the physiology of these neurons underlying memory formation since the mutant memory deficit was rescued with expression of a gish cDNA in these neurons only during adulthood. A cellular memory trace, detected as increased calcium influx into the α'/β' neuron processes in response to the odor used for conditioning, was disrupted in gish mutants. Epistasis experiments indicated a lack of genetic interactions between gish and rutabaga. Therefore, gish participates in a rutabaga-independent pathway for memory formation and accounts for some of the residual learning that occurs in rutabaga mutants.
authors.affiliation: Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX 77030, USA. yingt@bcm.edu
authors.forename: Ying
authors.initials: Y
authors.lastname: Tan
authors.forename: Dinghui
authors.initials: D
authors.lastname: Yu
authors.forename: Jennifer
authors.initials: J
authors.lastname: Pletting
authors.forename: Ronald L
authors.initials: RL
authors.lastname: Davis
mesh_terms: D000262:Adenylyl Cyclases / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D030801:Animals, Genetically Modified; D001362:Avoidance Learning / Q000502:physiology*; D001522:Behavior, Animal; D047389:Casein Kinase I / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D003214:Conditioning, Classical / Q000502:physiology; D004330:Drosophila; D029721:Drosophila Proteins / Q000172:deficiency / Q000235:genetics / Q000378:metabolism* / Q000502:physiology*; D005786:Gene Expression Regulation / Q000235:genetics; D008569:Memory Disorders / Q000235:genetics; D024521:Mushroom Bodies / Q000254:growth & development / Q000378:metabolism; D009154:Mutation / Q000235:genetics; D009812:Odorants; D012903:Smell / Q000502:physiology*
chemical_list: D029721:Drosophila Proteins; D047389:Casein Kinase I; C479663:gish protein, Drosophila; D000262:Adenylyl Cyclases; C479926:Rut protein, Drosophila
references.citation: J Neurosci. 2003 Nov 19;23(33):10495-502
references.pmid: 14627633
references.citation: Cell Mol Life Sci. 1999 Apr;55(4):534-48
references.pmid: 10357224
references.citation: J Biol Chem. 1990 Aug 25;265(24):14264-9
references.pmid: 2117608
references.citation: Cell. 2001 Jun 15;105(6):757-68
references.pmid: 11440718
references.citation: Physiol Rev. 1996 Apr;76(2):299-317
references.pmid: 8618959
references.citation: Nature. 1998 Jan 29;391(6666):455-60
references.pmid: 9461212
references.citation: Neuron. 1992 Oct;9(4):619-27
references.pmid: 1382471
references.citation: J Neurosci. 1989 Jun;9(6):1883-96
references.pmid: 2542481
references.citation: Neuron. 2006 Dec 7;52(5):845-55
references.pmid: 17145505
references.citation: Dev Dyn. 2000 Jun;218(2):260-79
references.pmid: 10842356
references.citation: Neuron. 1999 Aug;23(4):787-98
references.pmid: 10482244
references.citation: Curr Opin Cell Biol. 1999 Oct;11(5):549-53
references.pmid: 10508654
references.citation: Curr Biol. 2005 Apr 12;15(7):603-15
references.pmid: 15823532
references.citation: Proc Natl Acad Sci U S A. 1984 Jun;81(11):3577-81
references.pmid: 6427773
references.citation: Neuron. 1996 Nov;17(5):931-44
references.pmid: 8938125
references.citation: Curr Opin Neurobiol. 2004 Jun;14(3):311-7
references.pmid: 15194111
references.citation: J Neurobiol. 1996 Sep;31(1):88-102
references.pmid: 9120439
references.citation: Neuron. 2007 Jan 4;53(1):103-15
references.pmid: 17196534
references.citation: Neuron. 2004 May 13;42(3):437-49
references.pmid: 15134640
references.citation: J Neurosci. 2007 Jul 18;27(29):7640-7
references.pmid: 17634358
references.citation: Nat Rev Neurosci. 2002 Mar;3(3):175-90
references.pmid: 11994750
references.citation: J Biol Chem. 1995 May 26;270(21):12717-24
references.pmid: 7759525
references.citation: Cell. 2005 Dec 2;123(5):945-57
references.pmid: 16325586
references.citation: Neuron. 1991 Mar;6(3):455-67
references.pmid: 1848082
references.citation: Proc Natl Acad Sci U S A. 2004 Jan 6;101(1):198-203
references.pmid: 14684832
references.citation: Dev Biol. 2006 Nov 1;299(1):221-37
references.pmid: 16987508
references.citation: Neuron. 2002 Aug 29;35(5):951-60
references.pmid: 12372288
references.citation: J Neurosci. 2008 Apr 23;28(17):4368-76
references.pmid: 18434515
references.citation: Cell Signal. 1998 Nov;10(10):699-711
references.pmid: 9884021
references.citation: Proc Natl Acad Sci U S A. 1986 Dec;83(24):9313-7
references.pmid: 3025834
references.citation: J Comp Physiol A. 1985 Sep;157(2):263-77
references.pmid: 3939242
references.citation: Genes Dev. 1993 Jul;7(7B):1447-58
references.pmid: 7687228
references.citation: J Cell Biol. 1984 Dec;99(6):2082-91
references.pmid: 6501413
references.citation: Neuron. 1993 Aug;11(2):197-208
references.pmid: 8352940
references.citation: Science. 2003 Dec 5;302(5651):1765-8
references.pmid: 14657498
references.citation: Adv Second Messenger Phosphoprotein Res. 1991;23:123-64
references.pmid: 1997039
references.citation: Neuron. 2002 Jan 17;33(2):193-203
references.pmid: 11804568
references.citation: Nature. 1981 Jan 1;289(5793):79-81
references.pmid: 6256649
references.citation: Science. 1976 Dec 10;194(4270):1178-81
references.pmid: 186870
references.citation: Cell. 1992 Feb 7;68(3):479-89
references.pmid: 1739965
references.citation: Biochem Biophys Res Commun. 1992 Feb 14;182(3):1460-5
references.pmid: 1540189