title: Genetic manipulation of Mycobacterium abscessus.
abstract: This unit covers genetic manipulation protocols for M. abscessus, including nucleic acid extraction (plasmid DNA, genomic DNA, and RNA), transformation, and a recombineering mutagenesis method. M. abscessus is a Biosafety Level 2 (BSL-2) bacterium, and working considerations are also discussed.
authors.affiliation: Université Paris Descartes, Faculté de Médecine Paris Descartes, Paris, France.
authors.forename: Halima
authors.initials: H
authors.lastname: Medjahed
authors.forename: Anil Kumar
authors.initials: AK
authors.lastname: Singh
mesh_terms: D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D005818:Genetic Engineering / Q000379:methods*; D005827:Genetics, Microbial / Q000379:methods*; D016296:Mutagenesis; D009161:Mycobacterium / Q000235:genetics*; D010957:Plasmids; D012329:RNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D011995:Recombination, Genetic; D014169:Transformation, Bacterial
chemical_list: D004269:DNA, Bacterial; D012329:RNA, Bacterial