title: Noninvasive neuron pinning with nanopillar arrays.
abstract: Cell migration in a cultured neuronal network presents an obstacle to selectively measuring the activity of the same neuron over a long period of time. Here we report the use of nanopillar arrays to pin the position of neurons in a noninvasive manner. Vertical nanopillars protruding from the surface serve as geometrically better focal adhesion points for cell attachment than a flat surface. The cell body mobility is significantly reduced from 57.8 μm on a flat surface to 3.9 μm on nanopillars over a 5 day period. Yet, neurons growing on nanopillar arrays show a growth pattern that does not differ in any significant way from that seen on a flat substrate. Notably, while the cell bodies of neurons are efficiently anchored by the nanopillars, the axons and dendrites are free to grow and elongate into the surrounding area to develop a neuronal network, which opens up opportunities for long-term study of the same neurons in connected networks.
authors.affiliation: Department of Material Science and Engineering, Stanford University, Stanford, California 94305, USA.
authors.forename: Chong
authors.initials: C
authors.lastname: Xie
authors.forename: Lindsey
authors.initials: L
authors.lastname: Hanson
authors.forename: Wenjun
authors.initials: W
authors.lastname: Xie
authors.forename: Ziliang
authors.initials: Z
authors.lastname: Lin
authors.forename: Bianxiao
authors.initials: B
authors.lastname: Cui
authors.forename: Yi
authors.initials: Y
authors.lastname: Cui
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000379:methods; D002465:Cell Movement; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D009474:Neurons / Q000166:cytology*; D051381:Rats; D012825:Silicon / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D012822:Silicon Dioxide; D012825:Silicon
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