title: CCL20 is overexpressed in Mycobacterium tuberculosis-infected monocytes and inhibits the production of reactive oxygen species (ROS).
abstract: CCL20 is a chemokine that attracts immature dendritic cells. We show that monocytes, cells characteristic of the innate immune response, infected with Mycobacterium tuberculosis express the CCL20 gene at a much higher level than the same cells infected with non-tuberculous mycobacteria. Interferon (IFN)-γ, a fundamental cytokine in the immune response to tuberculosis, strongly inhibits both the transcription and the translation of CCL20. We have also confirmed that dendritic cells are a suitable host for mycobacteria proliferation, although CCL20 does not seem to influence their intracellular multiplication rate. The chemokine, however, down-regulates the characteristic production of reactive oxygen species (ROS) induced by M. tuberculosis in monocytes, which may affect the activity of the cells. Apoptosis mediated by the mycobacteria, possibly ROS-dependent, was also inhibited by CCL20.
authors.affiliation: Fundación Instituto de Estudios de Ciencias de la Salud de Castilla y León, Hospital de León, Spain. omrivero@iecscyl.com
authors.forename: O M
authors.initials: OM
authors.lastname: Rivero-Lezcano
authors.forename: C
authors.initials: C
authors.lastname: González-Cortés
authors.forename: D
authors.initials: D
authors.lastname: Reyes-Ruvalcaba
authors.forename: C
authors.initials: C
authors.lastname: Diez-Tascón
mesh_terms: D000906:Antibodies / Q000276:immunology / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects / Q000276:immunology; D018932:Chemokine CCL2 / Q000276:immunology / Q000378:metabolism; D054418:Chemokine CCL20 / Q000235:genetics / Q000276:immunology / Q000378:metabolism* / Q000494:pharmacology; D019742:Chemokines, CC / Q000235:genetics; D002633:Chemotaxis / Q000187:drug effects / Q000276:immunology; D015169:Colony Count, Microbial; D017077:Culture Media, Conditioned / Q000494:pharmacology; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology / Q000382:microbiology; D015870:Gene Expression / Q000235:genetics; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D016952:Legionella pneumophila / Q000166:cytology / Q000276:immunology; D008264:Macrophages / Q000187:drug effects / Q000276:immunology / Q000382:microbiology; D009000:Monocytes / Q000187:drug effects / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology*; D009162:Mycobacterium avium / Q000166:cytology / Q000276:immunology; D019909:Mycobacterium kansasii / Q000166:cytology / Q000276:immunology; D009169:Mycobacterium tuberculosis / Q000166:cytology / Q000276:immunology*; D017382:Reactive Oxygen Species / Q000378:metabolism*
chemical_list: D000906:Antibodies; C111835:CCL2 protein, human; C108355:CCL20 protein, human; D018932:Chemokine CCL2; D054418:Chemokine CCL20; D019742:Chemokines, CC; D017077:Culture Media, Conditioned; D017382:Reactive Oxygen Species; D007371:Interferon-gamma
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