Core3 glycan as tumor suppressor.
Recent studies demonstrated that mucin type O-glycans have important roles in tumorigenesis. Although several papers have been reported that increased core2 O-glycan is detected with several cancer progression (Dalziel, et al., 2001; Machida, et al., 2001), very little is known about the function of core3 O-glycan in tumorigenesis. Core3 O-glycan is synthesized by beta1, 3-N-acetylglucosamintraseferase 6 (core3 synthase). To understand the function of core3 O-glycan in cancer, ectopic expression of core3 synthase to prostate cancer cells were used for tumor formation assay. Since core3 expressing prostate cancer cells show decreased tumor formation and metastasis to lymph node through attenuating the maturation and heterodimerization of integrin alpha2beta1, those findings indicate that core3 structure acts as tumor suppressor through regulating the integrin functions. In this chapter, we discuss methods used to reveal the mechanisms how core3 glycan act as tumor suppressor.
Lee
Seung Ho
SH
Tumor Microenvironment Program, Cancer Research Center, Sanford-Burnham Medical Research Institute, La Jolla, California, USA.
Fukuda
Minoru
M
D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D017351:N-Acetylglucosaminyltransferases / Q000378:metabolism; D011134:Polysaccharides / Q000235:genetics / Q000378:metabolism*; D045664:Proprotein Convertase 1 / Q000378:metabolism; D011471:Prostatic Neoplasms / Q000503:physiopathology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism*
D011134:Polysaccharides; D025521:Tumor Suppressor Proteins; D017351:N-Acetylglucosaminyltransferases; D045664:Proprotein Convertase 1