title: Effect of acidic pH on the invasion efficiency and the type III secretion system of Burkholderia thailandensis.
abstract: Burkholderia thailandensis is a close relative of Burkholderia pseudomallei. These organisms are very similar, but B. thailandensis is far less virulent than B. pseudomallei. Nucleotide sequencing and analysis of 14 B. thailandensis isolates revealed variation in the regions coding for the type III secreted BipD protein. The degree of B. thailandensis BipD sequence variation was greater than that found in B. pseudomallei. Western blot analysis indicated that, unlike B. pseudomallei, B. thailandensis type III secreted proteins including BipD and BopE could not be detected in the supernatant of culture medium unless induced by acidic conditions. In addition, culturing B. thailandensis under acidic growth conditions (pH 4.5) can induce the ability of this bacterium to invade human respiratory epithelial cells A549. The identification of an environmental stimulus that increases the invasion capability of B. thailandensis invasion is of value for those who would like to use this bacterium as a model to study B. pseudomallei virulence.
authors.affiliation: Department of Immunology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
authors.forename: Siroj
authors.initials: S
authors.lastname: Jitprasutwit
authors.forename: Wisansanee
authors.initials: W
authors.lastname: Thaewpia
authors.forename: Veerachat
authors.initials: V
authors.lastname: Muangsombut
authors.forename: Aroonlug
authors.initials: A
authors.lastname: Lulitanond
authors.forename: Chanvit
authors.initials: C
authors.lastname: Leelayuwat
authors.forename: Ganjana
authors.initials: G
authors.lastname: Lertmemongkolchai
authors.forename: Sunee
authors.initials: S
authors.lastname: Korbsrisate
mesh_terms: D000143:Acids / Q000494:pharmacology*; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D019117:Burkholderia / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity*; D019121:Burkholderia Infections / Q000382:microbiology*; D004847:Epithelial Cells / Q000382:microbiology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D014774:Virulence
chemical_list: D000143:Acids; D001426:Bacterial Proteins
references.citation: Infect Immun. 2008 Nov;76(11):5402-11
references.pmid: 18779342
references.citation: Microbiology (Reading). 2004 Aug;150(Pt 8):2669-2676
references.pmid: 15289563
references.citation: Infect Immun. 2005 Mar;73(3):1432-40
references.pmid: 15731041
references.citation: J Mol Biol. 2006 Oct 13;363(1):125-36
references.pmid: 16950399
references.citation: J Med Microbiol. 1996 Dec;45(6):408-12
references.pmid: 8958243
references.citation: Mol Microbiol. 1999 Aug;33(4):806-16
references.pmid: 10447889
references.citation: Mol Microbiol. 2002 Nov;46(3):649-59
references.pmid: 12410823
references.citation: Adv Exp Med Biol. 2007;603:211-6
references.pmid: 17966417
references.citation: J Clin Microbiol. 1999 Jun;37(6):1906-12
references.pmid: 10325345
references.citation: Microb Pathog. 2004 May;36(5):287-92
references.pmid: 15043863
references.citation: J Med Microbiol. 2002 May;51(5):374-384
references.pmid: 11990489
references.citation: J Bacteriol. 1995 Jul;177(14):3965-71
references.pmid: 7608068
references.citation: Clin Infect Dis. 1999 Apr;28(4):927-8
references.pmid: 10825075
references.citation: Epidemiol Infect. 1997 Apr;118(2):137-48
references.pmid: 9129590
references.citation: BMC Immunol. 2009 Apr 27;10:20
references.pmid: 19397822
references.citation: Microb Pathog. 2001 May;30(5):253-69
references.pmid: 11373120
references.citation: Arch Microbiol. 2008 Dec;190(6):623-31
references.pmid: 18654761
references.citation: BMC Microbiol. 2006 May 26;6:46
references.pmid: 16725056
references.citation: Bioinformatics. 2009 May 1;25(9):1189-91
references.pmid: 19151095
references.citation: Infect Immun. 1997 Oct;65(10):4319-21
references.pmid: 9317042