title: A new crystal form of Lys48-linked diubiquitin.
abstract: Lys48-linked polyubiquitin chains are recognized by the proteasome as a tag for the degradation of the attached substrates. Here, a new crystal form of Lys48-linked diubiquitin (Ub2) was obtained and the crystal structure was refined to 1.6 A resolution. The structure reveals an ordered isopeptide bond in a trans configuration. All three molecules in the asymmetric unit were in the same closed conformation, in which the hydrophobic patches of both the distal and the proximal moieties interact with each other. Despite the different crystallization conditions and different crystal packing, the new crystal structure of Ub2 is similar to the previously published structure of diubiquitin, but differences are observed in the conformation of the flexible isopeptide linkage.
authors.affiliation: Department of Biochemistry, McGill University, 3649 Promenade Sir William Osler, Montreal, Québec H3G 0B1, Canada. jean.trempe@mail.mcgill.ca
authors.forename: Jean François
authors.initials: JF
authors.lastname: Trempe
authors.forename: Nicholas R
authors.initials: NR
authors.lastname: Brown
authors.forename: Martin E M
authors.initials: ME
authors.lastname: Noble
authors.forename: Jane A
authors.initials: JA
authors.lastname: Endicott
mesh_terms: D018360:Crystallography, X-Ray; D006801:Humans; D008239:Lysine / Q000737:chemistry; D008958:Models, Molecular; D020836:Protein Structure, Quaternary; D025801:Ubiquitin / Q000737:chemistry*
chemical_list: D025801:Ubiquitin; D008239:Lysine
references.citation: Nat Cell Biol. 2001 Oct;3(10):939-43
references.pmid: 11584278
references.citation: Nat Rev Mol Cell Biol. 2005 Aug;6(8):610-21
references.pmid: 16064137
references.citation: J Biomol NMR. 2006 Feb;34(2):101-15
references.pmid: 16518697
references.citation: Mol Cell. 2009 Aug 14;35(3):280-90
references.pmid: 19683493
references.citation: Nature. 2008 May 22;453(7194):548-52
references.pmid: 18497827
references.citation: Acta Crystallogr D Biol Crystallogr. 1997 Jul 1;53(Pt 4):448-55
references.pmid: 15299911
references.citation: Nat Struct Biol. 2001 May;8(5):417-22
references.pmid: 11323716
references.citation: Structure. 1996 Dec 15;4(12):1395-400
references.pmid: 8994966
references.citation: Acta Crystallogr D Biol Crystallogr. 2001 Feb;57(Pt 2):341-4
references.pmid: 11173499
references.citation: Proteins. 2006 Jun 1;63(4):787-96
references.pmid: 16609980
references.citation: J Biol Chem. 2002 Jan 4;277(1):516-27
references.pmid: 11675391
references.citation: EMBO J. 2005 Sep 21;24(18):3178-89
references.pmid: 16138082
references.citation: Mol Cell. 2009 Dec 25;36(6):1018-33
references.pmid: 20064467
references.citation: Acta Crystallogr D Biol Crystallogr. 2006 Jan;62(Pt 1):72-82
references.pmid: 16369096
references.citation: Acta Crystallogr D Biol Crystallogr. 1997 May 1;53(Pt 3):240-55
references.pmid: 15299926
references.citation: J Biol Chem. 1992 Aug 15;267(23):16467-71
references.pmid: 1322903
references.citation: J Mol Biol. 1987 Apr 5;194(3):531-44
references.pmid: 3041007
references.citation: Acta Crystallogr D Biol Crystallogr. 1999 Jan;55(Pt 1):191-205
references.pmid: 10089410
references.citation: Mol Cell. 2005 Jun 10;18(6):687-98
references.pmid: 15949443
references.citation: J Mol Biol. 2002 Dec 6;324(4):637-47
references.pmid: 12460567
references.citation: J Biol Chem. 1994 Mar 11;269(10):7059-61
references.pmid: 8125911
references.citation: J Biol Chem. 1990 Dec 15;265(35):21835-42
references.pmid: 2174887
references.citation: Proteins. 2005 Aug 15;60(3):367-81
references.pmid: 15937902
references.citation: Acta Crystallogr D Biol Crystallogr. 2006 Jan;62(Pt 1):48-57
references.pmid: 16369093
references.citation: Acta Crystallogr D Biol Crystallogr. 2004 Dec;60(Pt 12 Pt 1):2126-32
references.pmid: 15572765
references.citation: Nature. 2008 May 22;453(7194):481-8
references.pmid: 18497817
references.citation: J Mol Biol. 1994 Feb 18;236(2):601-9
references.pmid: 8107144
references.citation: J Appl Crystallogr. 2007 Aug 1;40(Pt 4):658-674
references.pmid: 19461840
references.citation: J Biol Chem. 1997 Sep 19;272(38):23712-21
references.pmid: 9295315
references.citation: Annu Rev Biochem. 2009;78:477-513
references.pmid: 19489727
references.citation: Annu Rev Biochem. 1998;67:425-79
references.pmid: 9759494