title: Binucleine 2, an isoform-specific inhibitor of Drosophila Aurora B kinase, provides insights into the mechanism of cytokinesis.
abstract: Aurora kinases are key regulators of cell division and important targets for cancer therapy. We report that Binucleine 2 is a highly isoform-specific inhibitor of Drosophila Aurora B kinase, and we identify a single residue within the kinase active site that confers specificity for Aurora B. Using Binucleine 2, we show that Aurora B kinase activity is not required during contractile ring ingression, providing insight into the mechanism of cytokinesis.
authors.forename: Yegor
authors.initials: Y
authors.lastname: Smurnyy
authors.forename: Angela V
authors.initials: AV
authors.lastname: Toms
authors.forename: Gilles R
authors.initials: GR
authors.lastname: Hickson
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Eck
authors.forename: Ulrike S
authors.initials: US
authors.lastname: Eggert
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D064107:Aurora Kinase B; D064127:Aurora Kinases; D018797:Cell Cycle Proteins / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000502:physiology*; D048749:Cytokinesis / Q000187:drug effects / Q000502:physiology*; D029721:Drosophila Proteins / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000502:physiology*; D004331:Drosophila melanogaster / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology*; D007527:Isoenzymes / Q000037:antagonists & inhibitors; D007532:Isoleucine / Q000737:chemistry; D008969:Molecular Sequence Data; D047428:Protein Kinase Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D017346:Protein Serine-Threonine Kinases / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000502:physiology*; D011720:Pyrazoles / Q000737:chemistry* / Q000494:pharmacology; D034622:RNA Interference; D012545:Schiff Bases / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D018797:Cell Cycle Proteins; D029721:Drosophila Proteins; D007527:Isoenzymes; D047428:Protein Kinase Inhibitors; D011720:Pyrazoles; D012545:Schiff Bases; C559086:binucleine 2; D007532:Isoleucine; C576957:AurB protein, Drosophila; D064107:Aurora Kinase B; D064127:Aurora Kinases; D017346:Protein Serine-Threonine Kinases
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