Analysis and difference of voltage-dependent anion channel mRNA in ejaculated spermatozoa from normozoospermic fertile donors and infertile patients with idiopathic asthenozoospermia.
OBJECTIVE
to analyze the abundance and difference of voltage-dependent anion channel (VDAC) mRNA in ejaculated spermatozoa from normozoospermic fertile donors and infertile patients with idiopathic asthenozoospermia.


METHODS
high motile and low motile spermatozoa were separated respectively from ejaculates of 36 donors and 40 patients using a discontinuous Percoll gradient centrifugation. Real-Time PCR was performed to detect mRNA abundance and difference of three VDAC subtypes between two groups with different sperm motility.


RESULTS
real-Time PCR demonstrated that three VDAC mRNAs were present in mature spermatozoa. The VDAC2 mRNA level in ejaculated spermatozoa of patients was significantly higher than that of donors. No significant differences of VDAC1 and VDAC3 mRNA levels were found between two groups.


CONCLUSIONS
the high abundance of VDAC2 mRNA seemed to have a positive correlation with low sperm motility. The abnormal expression of VDAC might be related to male infertility with idiopathic asthenozoospermia.
Liu
Bianjiang
B
Laboratory of Reproductive Medicine, Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
Wang
Peng
P
Wang
Zengjun
Z
Jia
Yuejun
Y
Niu
Xiaobing
X
Wang
Wei
W
Zhang
Wei
W
D000328:Adult; D053627:Asthenozoospermia / Q000235:genetics*; D016022:Case-Control Studies; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism; D013081:Sperm Motility; D013094:Spermatozoa / Q000378:metabolism*; D050996:Voltage-Dependent Anion Channel 2 / Q000378:metabolism*; D055815:Young Adult
D012333:RNA, Messenger; C494765:VDAC2 protein, human; D050996:Voltage-Dependent Anion Channel 2
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