Effect of some natural mineral waters in nutrient uptake by Caco-2 cells.
We studied the effect of some mineral waters and some of their constituents on the apical uptake of (14)C-butyrate ((14)C-BT) and (3)H-O-methyl-D-glucose ((3)H-OMG) by Caco-2 cells. Uptake of (14)C-BT increased after a 20-minute exposure to 1 % (v/v) distilled water, and, compared to distilled water, it was decreased by Pedras Salgadas((R)) 1 % (v/v) and Melgaço((R)) 5 % (v/v), and increased by Vidago((R)) 5 % (v/v). Moreover, it increased after a 48-hour exposure to Vidago((R)) or Melgaço((R)) waters (5 % (v/v)). Also, uptake of (14)C-BT was reduced after a 20-minute exposure to MgCl(2), MgSO(4), or CaCl(2). Uptake of (3)H-OMG was reduced after a 20-minute exposure to Melgaço((R)) water [1 % (v/v)], when compared to distilled water. Also, a 48-hour exposure to Pedras Salgadas((R)) or Melgaço((R)) water (5 % (v/v)) increased and decreased uptake, respectively. Finally, uptake of (3)H-OMG decreased after a 20-minute exposure to MgSO(4) or NaF. In conclusion, uptake of (14)C-BT and (3)H-OMG by Caco-2 cells is differently modulated by distinct mineral waters.
Gonçalves
Pedro
P
Department of Biochemistry, Faculty of Medicine, University of Porto, Porto, Portugal.
Araújo
João R
JR
Martel
Fátima
F
D019325:3-O-Methylglucose / Q000378:metabolism*; D002087:Butyrates / Q000378:metabolism*; D018938:Caco-2 Cells; D002478:Cells, Cultured; D006801:Humans; D008900:Mineral Waters; D013997:Time Factors
D002087:Butyrates; D008900:Mineral Waters; D019325:3-O-Methylglucose