Diffusion of particles in the extracellular matrix: the effect of repulsive electrostatic interactions.
Diffusive transport of macromolecules and nanoparticles in charged fibrous media is of interest in many biological applications, including drug delivery and separation processes. Experimental findings have shown that diffusion can be significantly hindered by electrostatic interactions between the diffusing particle and charged components of the extracellular matrix. The implications, however, have not been analyzed rigorously. Here, we present a mathematical framework to study the effect of charge on the diffusive transport of macromolecules and nanoparticles in the extracellular matrix of biological tissues. The model takes into account steric, hydrodynamic, and electrostatic interactions. We show that when the fiber size is comparable to the Debye length, electrostatic forces between the fibers and the particles result in slowed diffusion. However, as the fiber diameter increases the repulsive forces become less important. Our results explain the experimental observations that neutral particles diffuse faster than charged particles. Taken together, we conclude that optimal particles for delivery to tumors should be initially cationic to target the tumor vessels and then change to neutral charge after exiting the blood vessels.
Stylianopoulos
Triantafyllos
T
Edwin L. Steele Laboratory, Department of Radiation Oncology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA.
Poh
Ming-Zher
MZ
Insin
Numpon
N
Bawendi
Moungi G
MG
Fukumura
Dai
D
Munn
Lance L
LL
Jain
Rakesh K
RK
D000818:Animals; D003094:Collagen / Q000737:chemistry / Q000378:metabolism; D004058:Diffusion; D016503:Drug Delivery Systems; D005109:Extracellular Matrix / Q000378:metabolism*; D057446:Hydrodynamics; D008954:Models, Biological; D008958:Models, Molecular; D053758:Nanoparticles / Q000737:chemistry; D009369:Neoplasms / Q000378:metabolism / Q000473:pathology; D009994:Osmolar Concentration; D010316:Particle Size; D051381:Rats; D015203:Reproducibility of Results; D055672:Static Electricity
D003094:Collagen
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