Effect of acidic pH on the invasion efficiency and the type III secretion system of Burkholderia thailandensis.
Burkholderia thailandensis is a close relative of Burkholderia pseudomallei. These organisms are very similar, but B. thailandensis is far less virulent than B. pseudomallei. Nucleotide sequencing and analysis of 14 B. thailandensis isolates revealed variation in the regions coding for the type III secreted BipD protein. The degree of B. thailandensis BipD sequence variation was greater than that found in B. pseudomallei. Western blot analysis indicated that, unlike B. pseudomallei, B. thailandensis type III secreted proteins including BipD and BopE could not be detected in the supernatant of culture medium unless induced by acidic conditions. In addition, culturing B. thailandensis under acidic growth conditions (pH 4.5) can induce the ability of this bacterium to invade human respiratory epithelial cells A549. The identification of an environmental stimulus that increases the invasion capability of B. thailandensis invasion is of value for those who would like to use this bacterium as a model to study B. pseudomallei virulence.
Jitprasutwit
Siroj
S
Department of Immunology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
Thaewpia
Wisansanee
W
Muangsombut
Veerachat
V
Lulitanond
Aroonlug
A
Leelayuwat
Chanvit
C
Lertmemongkolchai
Ganjana
G
Korbsrisate
Sunee
S
D000143:Acids / Q000494:pharmacology*; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D019117:Burkholderia / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity*; D019121:Burkholderia Infections / Q000382:microbiology*; D004847:Epithelial Cells / Q000382:microbiology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D014774:Virulence
D000143:Acids; D001426:Bacterial Proteins
Infect Immun. 2008 Nov;76(11):5402-11
18779342
Microbiology (Reading). 2004 Aug;150(Pt 8):2669-2676
15289563
Infect Immun. 2005 Mar;73(3):1432-40
15731041
J Mol Biol. 2006 Oct 13;363(1):125-36
16950399
J Med Microbiol. 1996 Dec;45(6):408-12
8958243
Mol Microbiol. 1999 Aug;33(4):806-16
10447889
Mol Microbiol. 2002 Nov;46(3):649-59
12410823
Adv Exp Med Biol. 2007;603:211-6
17966417
J Clin Microbiol. 1999 Jun;37(6):1906-12
10325345
Microb Pathog. 2004 May;36(5):287-92
15043863
J Med Microbiol. 2002 May;51(5):374-384
11990489
J Bacteriol. 1995 Jul;177(14):3965-71
7608068
Clin Infect Dis. 1999 Apr;28(4):927-8
10825075
Epidemiol Infect. 1997 Apr;118(2):137-48
9129590
BMC Immunol. 2009 Apr 27;10:20
19397822
Microb Pathog. 2001 May;30(5):253-69
11373120
Arch Microbiol. 2008 Dec;190(6):623-31
18654761
BMC Microbiol. 2006 May 26;6:46
16725056
Bioinformatics. 2009 May 1;25(9):1189-91
19151095
Infect Immun. 1997 Oct;65(10):4319-21
9317042