Proteome analysis of Arabidopsis seedlings exposed to bacterial volatiles.
Plant root-associated bacteria (rhizobacteria) elicit plant basal immunity referred to as induced systemic resistance (ISR) against multiple pathogens. Among multi-bacterial determinants involving such ISR, the induction of ISR and promotion of growth by bacterial volatile compounds was previously reported. To exploit global de novo expression of plant proteins by bacterial volatiles, proteomic analysis was performed after exposure of Arabidopsis plants to the rhizobacterium Bacillus subtilis GB03. Ethylene biosynthesis enzymes were significantly up-regulated. Analysis by quantitative reverse transcriptase polymerase chain reaction confirmed that ethylene biosynthesis-related genes SAM-2, ACS4, ACS12, and ACO2 as well as ethylene response genes, ERF1, GST2, and CHIB were up-regulated by the exposure to bacterial volatiles. More interestingly, the emission of bacterial volatiles significantly up-regulated both key defense mechanisms mediated by jasmonic acid and salicylic acid signaling pathways. In addition, high accumulation of antioxidant proteins also provided evidence of decreased sensitivity to reactive oxygen species during the elicitation of ISR by bacterial volatiles. The present results suggest that the proteomic analysis of plant defense responses in bacterial volatile-mediated ISR can reveal the mechanisms of plant basal defenses orchestrated by endogenous ethylene production pathways and the generation of reactive oxygen species.
Kwon
Young Sang
YS
Department of Applied Biology and Research Institute of Life Science, Gyeongsang National University, Jinju, 660-701, South Korea.
Ryu
Choong-Min
CM
Lee
Soohyun
S
Park
Hyo Bee
HB
Han
Ki Soo
KS
Lee
Jung Han
JH
Lee
Kyunghee
K
Chung
Woo Sik
WS
Jeong
Mi-Jeong
MJ
Kim
Hee Kyu
HK
Bae
Dong-Won
DW
D000975:Antioxidants / Q000378:metabolism; D017360:Arabidopsis / Q000254:growth & development / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D001412:Bacillus subtilis / Q000378:metabolism*; D001483:Base Sequence; D002734:Chlorophyll / Q000378:metabolism; D017931:DNA Primers; D020543:Proteome; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D055549:Volatile Organic Compounds / Q000378:metabolism*
D000975:Antioxidants; D029681:Arabidopsis Proteins; D017931:DNA Primers; D020543:Proteome; D055549:Volatile Organic Compounds; D002734:Chlorophyll
FEMS Microbiol Lett. 2005 Oct 1;251(1):1-7
16099604
Plant Physiol. 2010 Mar;152(3):1562-73
20081042
Trends Plant Sci. 2006 Apr;11(4):184-91
16531096
Proc Natl Acad Sci U S A. 2000 Jul 18;97(15):8711-6
10890883
Mol Plant. 2008 May;1(3):496-509
19825556
New Phytol. 2010 Jan;185(2):554-67
19906091
Proteomics. 2004 Nov;4(11):3343-52
15378737
Curr Opin Plant Biol. 2007 Aug;10(4):372-9
17646123
Plant Physiol. 1986 Nov;82(3):742-7
16665104
Phytopathology. 2004 Nov;94(11):1259-66
18944464
Plant Physiol. 2004 Mar;134(3):1017-26
14976231
Mol Plant Microbe Interact. 2008 Aug;21(8):1067-75
18616403
Biochem J. 1976 Jun 1;155(3):697-700
182136
Plant Physiol. 1994 Jan;104(1):171-178
12232070
Plant J. 2004 Aug;39(3):381-92
15255867
Phytopathology. 2009 May;99(5):506-11
19351246
Annu Rev Phytopathol. 2005;43:545-80
16078895
Plant J. 2009 May;58(4):568-77
19154225
Trends Plant Sci. 2002 Sep;7(9):405-10
12234732
Plant J. 2002 Sep;31(5):589-99
12207649
J Sep Sci. 2008 Jun;31(11):2032-9
18615819
Plant J. 2008 Oct;56(2):264-273
18573192
New Phytol. 2010 Mar;185(4):1062-73
20085621
J Biol Chem. 1990 Aug 15;265(23):13683-6
1696256
Proc Natl Acad Sci U S A. 2003 Apr 15;100(8):4927-32
12684534
Proc Natl Acad Sci U S A. 2007 Dec 11;104(50):20131-6
18056646
Trends Plant Sci. 2009 Jan;14(1):1-4
19056309
Planta. 2007 Sep;226(4):839-51
17497164
Electrophoresis. 1998 Aug;19(11):1853-61
9740045
Proteomics. 2007 Nov;7(21):3943-52
17902191
Proteome Sci. 2009 Dec 24;7:47
20034395
Pest Manag Sci. 2009 May;65(5):497-503
19206090
Mol Plant Microbe Interact. 2006 May;19(5):502-11
16673937
J Biol Chem. 1969 Nov 25;244(22):6056-63
4981789
Science. 2006 Apr 21;312(5772):436-9
16627744
Annu Rev Cell Dev Biol. 2000;16:1-18
11031228