title: [Everted intestinal sac method for quick finding absorption ingredients of Wuzhuyu decoction].
abstract: OBJECTIVE
  To establish a method for quick finding the absorption ingredients of Wuzhuyu decoction in order to select the index to control its quality.
  
  
  METHODS
  The absorption of three concentration of Wuzhuyu decotion was investigated with the in vitro-everted intestinal sac model. The intestinal bag fluid of jejunum and ileum were collected in different time and the eight ingredients, which were evodiamine (Ev), rutaecarpine (Ru), limonin (Li), ginsenoside-Rb1, -Rg1, -Re (Rb1, Rg1, Re), isorhamnetin-3-O-beta-D-glucosyl(6''-->1'")-alpha-L-rhamnoside (Irs)and 6-gingerol (6-Gi), were detected by HPLC as the represent constituents in samples.
  
  
  RESULTS
  Eight ingredients except Ru in samples could be detected, but Ev could not be detected in high concentration samples. The ratios between absorption ingredients were different from in Wuzhuyu decotion.
  
  
  CONCLUSIONS
  The in vitro-everted intestinal sac canc absorb the ingredients of Wuzhuyu decotion selectivity. Compare with the ileum, the jejunum can provide the more absorption information and faster, the best test time is 60-90 min.
authors.affiliation: School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China.
authors.forename: Muxin
authors.initials: M
authors.lastname: Gong
authors.forename: Yaxun
authors.initials: Y
authors.lastname: Wang
authors.forename: Yafang
authors.initials: Y
authors.lastname: Song
authors.forename: Zhimin
authors.initials: Z
authors.lastname: Wang
authors.forename: Qiwei
authors.initials: Q
authors.lastname: Zhang
authors.forename: Weihao
authors.initials: W
authors.lastname: Wang
authors.forename: Jingjing
authors.initials: J
authors.lastname: Zhu
mesh_terms: D000818:Animals; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000493:pharmacokinetics*; D045604:Extracellular Fluid / Q000737:chemistry* / Q000187:drug effects; D007082:Ileum / Q000737:chemistry / Q000187:drug effects; D007408:Intestinal Absorption; D007583:Jejunum / Q000737:chemistry / Q000187:drug effects; D008297:Male; D051381:Rats; D017208:Rats, Wistar
chemical_list: D004365:Drugs, Chinese Herbal