title: Targeting the reversibly oxidized protein tyrosine phosphatase superfamily.
abstract: Controlled production of reactive oxygen species leads to reversible oxidation of protein tyrosine phosphatases (PTPs) and has emerged as an important tier of regulation over phosphorylation-dependent signal transduction. We present a modified cysteinyl-labeling assay that detects reversible oxidation of members of each of the different PTP subclasses. Here, we describe the methods for enriching reversibly oxidized PTPs from complex protein extracts, illustrating the procedure in IMR90 fibroblasts.
authors.affiliation: Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA.
authors.forename: Benoit
authors.initials: B
authors.lastname: Boivin
authors.forename: Ming
authors.initials: M
authors.lastname: Yang
authors.forename: Nicholas K
authors.initials: NK
authors.lastname: Tonks
mesh_terms: D000818:Animals; D006801:Humans; D010084:Oxidation-Reduction; D010766:Phosphorylation; D017027:Protein Tyrosine Phosphatases / Q000032:analysis* / Q000737:chemistry* / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000737:chemistry* / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology
chemical_list: D017382:Reactive Oxygen Species; D017027:Protein Tyrosine Phosphatases
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