Platelet-rich fibrin is a Bioscaffold and reservoir of growth factors for tissue regeneration.
The platelet-rich fibrin (PRF) is known as a rich source of autologous cytokines and growth factors and universally used for tissue regeneration in current clinical medicine. However, the microstructure of PRF has not been fully investigated nor have been studied the key molecules that differ PRF from platelet-rich plasma. We fabricated PRF under Choukroun's protocol and produced its extract (PRFe) by freezing at -80°C. The conventional histological, immunohistological staining, and scanning electron microscopy images showed the microstructure of PRF, appearing as two zones, the zone of platelets and the zone of fibrin, which resembled a mesh containing blood cells. The PRFe increased proliferation, migration, and promoted differentiation of the human alveolar bone marrow stem cells (hABMSCs) at 0.5% concentration in vitro. From the results of proteome array, matrix metalloproteinase 9 (MMP9) and Serpin E1 were detected especially in PRFe but not in concentrated platelet-rich plasma. Simultaneous elevation of MMP9, CD44, and transforming growth factor β-1 receptor was shown at 0.5% PRFe treatment to the hABMSC in immunoblot. Mineralization assay showed that MMP9 directly regulated mineralization differentiation of hABMSC. Transplantation of the fresh PRF into the mouse calvarias enhanced regeneration of the critical-sized defect. Our results strongly support the new characteristics of PRF as a bioscaffold and reservoir of growth factors for tissue regeneration.
Kang
Young-Ho
YH
Department of Oral and Maxillofacial Surgery, Dental Research Institute, School of Dentistry, Seoul National University, Seoul, Korea.
Jeon
Soung Hoo
SH
Park
Joo-Young
JY
Chung
Jong-Hoon
JH
Choung
Yun-Hoon
YH
Choung
Han-Wool
HW
Kim
Eun-Suk
ES
Choung
Pill-Hoon
PH
D000042:Absorption; D000818:Animals; D001861:Bone Regeneration / Q000502:physiology*; D002478:Cells, Cultured; D005337:Fibrin / Q000378:metabolism*; D048091:Guided Tissue Regeneration / Q000295:instrumentation*; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D053657:Platelet-Rich Plasma / Q000378:metabolism*; D012887:Skull Fractures / Q000473:pathology / Q000601:surgery*; D054457:Tissue Scaffolds; D016896:Treatment Outcome
D036341:Intercellular Signaling Peptides and Proteins; D005337:Fibrin