Reduced allele specific annexin A5 mRNA levels in placentas carrying the M2/ANXA5 allele.
We aimed to trace the allele specific expression of ANXA5 mRNA in placentas carrying the M2 haplotype, conferring higher recurrent pregnancy loss risk, in order to verify directly the role of M2 in the relevant organ. The M2 allele in heterozygous placentas results in an average of 42% reduced ANXA5 mRNA levels as compared to the normal allele. Protein levels in these samples show considerable variations, impossible for statistical interpretation. The M2 allele of ANXA5 can be linked to reduced mRNA levels in heterozygous placentas and could result in more confined protein levels (lowered expression dynamics) of annexin A5.
Markoff
A
A
Institut für Medizinische Biochemie, ZMBE, WWU Münster, Von Esmarch Str 56, 48149 Münster, Germany. markoff@uni-muenster.de
Gerdes
S
S
Feldner
S
S
Bogdanova
N
N
Gerke
V
V
Grandone
E
E
D000026:Abortion, Habitual / Q000235:genetics* / Q000378:metabolism; D000483:Alleles; D017304:Annexin A5 / Q000235:genetics* / Q000378:metabolism; D015153:Blotting, Western; D005260:Female; D014644:Genetic Variation; D006801:Humans; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
D017304:Annexin A5; D012333:RNA, Messenger