title: Limited effect of dietary saturated fat on plasma saturated fat in the context of a low carbohydrate diet.
abstract: We recently showed that a hypocaloric carbohydrate restricted diet (CRD) had two striking effects: (1) a reduction in plasma saturated fatty acids (SFA) despite higher intake than a low fat diet, and (2) a decrease in inflammation despite a significant increase in arachidonic acid (ARA). Here we extend these findings in 8 weight stable men who were fed two 6-week CRD (12%en carbohydrate) varying in quality of fat. One CRD emphasized SFA (CRD-SFA, 86 g/d SFA) and the other, unsaturated fat (CRD-UFA, 47 g SFA/d). All foods were provided to subjects. Both CRD decreased serum triacylglycerol (TAG) and insulin, and increased LDL-C particle size. The CRD-UFA significantly decreased plasma TAG SFA (27.48 ± 2.89 mol%) compared to baseline (31.06 ± 4.26 mol%). Plasma TAG SFA, however, remained unchanged in the CRD-SFA (33.14 ± 3.49 mol%) despite a doubling in SFA intake. Both CRD significantly reduced plasma palmitoleic acid (16:1n-7) indicating decreased de novo lipogenesis. CRD-SFA significantly increased plasma phospholipid ARA content, while CRD-UFA significantly increased EPA and DHA. Urine 8-iso PGF(2α), a free radical-catalyzed product of ARA, was significantly lower than baseline following CRD-UFA (-32%). There was a significant inverse correlation between changes in urine 8-iso PGF(2α) and PL ARA on both CRD (r = -0.82 CRD-SFA; r = -0.62 CRD-UFA). These findings are consistent with the concept that dietary saturated fat is efficiently metabolized in the presence of low carbohydrate, and that a CRD results in better preservation of plasma ARA.
authors.affiliation: Department of Kinesiology, University of Connecticut, Unit 1110, Storrs, CT 06269-1110, USA.
authors.forename: Cassandra E
authors.initials: CE
authors.lastname: Forsythe
authors.forename: Stephen D
authors.initials: SD
authors.lastname: Phinney
authors.forename: Richard D
authors.initials: RD
authors.lastname: Feinman
authors.forename: Brittanie M
authors.initials: BM
authors.lastname: Volk
authors.forename: Daniel
authors.initials: D
authors.lastname: Freidenreich
authors.forename: Erin
authors.initials: E
authors.lastname: Quann
authors.forename: Kevin
authors.initials: K
authors.lastname: Ballard
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Puglisi
authors.forename: Carl M
authors.initials: CM
authors.lastname: Maresh
authors.forename: William J
authors.initials: WJ
authors.lastname: Kraemer
authors.forename: Douglas M
authors.initials: DM
authors.lastname: Bibus
authors.forename: Maria Luz
authors.initials: ML
authors.lastname: Fernandez
authors.forename: Jeff S
authors.initials: JS
authors.lastname: Volek
mesh_terms: D000328:Adult; D016718:Arachidonic Acid / Q000097:blood; D001835:Body Weight; D004032:Diet; D050528:Diet, Carbohydrate-Restricted; D004040:Dietary Carbohydrates / Q000008:administration & dosage* / Q000097:blood / Q000652:urine; D004041:Dietary Fats / Q000008:administration & dosage* / Q000097:blood* / Q000378:metabolism; D005227:Fatty Acids / Q000008:administration & dosage* / Q000097:blood* / Q000494:pharmacology; D006801:Humans; D008297:Male; D008875:Middle Aged
chemical_list: D004040:Dietary Carbohydrates; D004041:Dietary Fats; D005227:Fatty Acids; D016718:Arachidonic Acid
references.citation: Gastroenterology. 2009 May;136(5):1552-60
references.pmid: 19208352
references.citation: Lipids. 1997 Jul;32(7):697-705
references.pmid: 9252957
references.citation: Prog Lipid Res. 2008 Sep;47(5):307-18
references.pmid: 18396172
references.citation: Lipids. 2002 Jan;37(1):27-32
references.pmid: 11876260
references.citation: Am J Clin Nutr. 2009 May;89(5):1425-32
references.pmid: 19211817
references.citation: J Nutr. 2001 Feb;131(2):231-4
references.pmid: 11160538
references.citation: Diabetologia. 2005 Oct;48(10):1999-2005
references.pmid: 16132958
references.citation: Br Med J (Clin Res Ed). 1982 Oct 9;285(6347):993-6
references.pmid: 6812744
references.citation: J Nutr. 2006 May;136(5):1222-8
references.pmid: 16614408
references.citation: J Nutr. 2002 Jul;132(7):1879-85
references.pmid: 12097663
references.citation: Lipids. 1998 Feb;33(2):125-30
references.pmid: 9507233
references.citation: Eur J Clin Nutr. 2002 Feb;56(2):105-13
references.pmid: 11857043
references.citation: Am J Clin Nutr. 2010 Mar;91(3):535-46
references.pmid: 20071648
references.citation: Prog Lipid Res. 2008 Mar;47(2):77-106
references.pmid: 18177743
references.citation: J Cardiovasc Med (Hagerstown). 2007 Sep;8 Suppl 1:S46-9
references.pmid: 17876200
references.citation: J Lipid Res. 1998 Jun;39(6):1280-6
references.pmid: 9643360
references.citation: Nutr Metab Cardiovasc Dis. 2008 Jul;18(6):436-40
references.pmid: 18068341
references.citation: J Clin Endocrinol Metab. 2006 Feb;91(2):439-46
references.pmid: 16234304
references.citation: J Physiol Pharmacol. 2006 Dec;57(4):505-28
references.pmid: 17229978
references.citation: Nutr J. 2004 Jul 28;3:9
references.pmid: 15282028
references.citation: Am J Physiol. 1992 May;262(5 Pt 1):E631-6
references.pmid: 1590373
references.citation: Prog Lipid Res. 1997 Mar;36(1):1-21
references.pmid: 9373618
references.citation: Biochem Biophys Res Commun. 2000 Nov 30;278(3):797-9
references.pmid: 11095986
references.citation: Diabetologia. 2001 Mar;44(3):312-9
references.pmid: 11317662
references.citation: J Am Coll Nutr. 2000 Jun;19(3):383-91
references.pmid: 10872901
references.citation: Atherosclerosis. 2007 Jul;193(1):1-10
references.pmid: 17507020
references.citation: Lipids. 1997 Apr;32(4):449-56
references.pmid: 9113635
references.citation: JAMA. 2006 Feb 8;295(6):655-66
references.pmid: 16467234
references.citation: Biochim Biophys Acta. 1992 Dec 10;1180(2):147-62
references.pmid: 1463766
references.citation: Am J Clin Nutr. 2006 Feb;83(2):227-36
references.pmid: 16469979
references.citation: Lipids. 1997 Apr;32(4):421-5
references.pmid: 9113631
references.citation: Lipids. 2005 Dec;40(12):1207-13
references.pmid: 16477804
references.citation: Am Heart J. 2008 Dec;156(6):1117-23
references.pmid: 19033007
references.citation: Exp Clin Endocrinol Diabetes. 2006 May;114(5):249-56
references.pmid: 16804799
references.citation: Nutr Metab (Lond). 2007 Oct 31;4:23
references.pmid: 17974023
references.citation: J Nutr. 2005 Sep;135(9):2271-3
references.pmid: 16140910
references.citation: Circulation. 1990 Aug;82(2):495-506
references.pmid: 2372896
references.citation: Nutr Metab (Lond). 2005 Nov 16;2:31
references.pmid: 16288655
references.citation: Am J Clin Nutr. 1995 Mar;61(3 Suppl):721S-725S
references.pmid: 7879743
references.citation: Am J Cardiol. 2005 Aug 1;96(3):399-404
references.pmid: 16054467
references.citation: Am J Clin Nutr. 2006 May;83(5):1055-61
references.pmid: 16685046
references.citation: Am J Clin Nutr. 2007 Dec;86(6):1621-5
references.pmid: 18065578
references.citation: Atherosclerosis. 1998 Apr;137(2):367-76
references.pmid: 9622280
references.citation: Diabetes Care. 2008 Jan;31 Suppl 1:S61-78
references.pmid: 18165339
references.citation: Am J Clin Nutr. 2006 May;83(5):1025-31; quiz 1205
references.pmid: 16685042
references.citation: Am J Clin Nutr. 2005 Dec;82(6):1178-84
references.pmid: 16332649
references.citation: Lipids. 2009 Apr;44(4):297-309
references.pmid: 19082851
references.citation: J Nutr. 2003 Sep;133(9):2756-61
references.pmid: 12949361
references.citation: Lipids. 1997 Apr;32(4):427-33
references.pmid: 9113632
references.citation: Br J Nutr. 2007 Sep;98(3):451-3
references.pmid: 17705889
references.citation: Diabetologia. 2009 Apr;52(4):684-90
references.pmid: 19214471
references.citation: Am J Clin Nutr. 2010 Mar;91(3):502-9
references.pmid: 20089734
references.citation: Nutr Metab Cardiovasc Dis. 2003 Oct;13(5):256-66
references.pmid: 14717057
references.citation: Lipids. 2008 Jan;43(1):65-77
references.pmid: 18046594
references.citation: Metabolism. 1999 Nov;48(11):1402-8
references.pmid: 10582548