[Immunohistochemical expression of p53 oncoprotein in Wilms tumour in relation to histological components, histological types and preoperative chemotherapy].
BACKGROUND
There have been only few studies of immunoexpression of p53 in Wilms tumour (WT), and their results are somewhat contradictory.


OBJECTIVE
The aim of the study was to determine p53 immunohistochemical expression in WT in relation to its histological components, histological prognostic types classified according to the SIOP Working Classification of Renal Tumours of Childhood (2001), and influence of preoperative chemotherapy.


METHODS
The analyses are based on 79 primary WTs treated in single institution according to SIOP protocols between 1983-2001. For the immunohistochemical detection of p53, the monoclonal p53 antibody (DO-7, DAKO) was used. Semiquantitative grading of nuclear staining was done.


RESULTS
The immunoexpression of p53 was significantly higher in the blastemal and epithelial than in the stromal component (p < 0.001). It was significantly correlated to WT histological prognostic types (p = 0.039).The exensivity of p53 immunoexpression was higher in anaplastic components but a difference between WT type of diffuse anaplasia and all other types was nonsignificant (p = 0.10). Five blastemal type WTs were p53 immunopositive and four immunonegative. There was no difference in p53 immunopositivity between WT treated with the preoperative chemotherapy and primary resected WT (p = 0.88).


CONCLUSIONS
The immunoexpression of p53 in WT was significantly higher in the blastemal and epithelial than in the stromal component. It was in significant correlation with histological types of WT. The anaplastic component had noticeable but statistically not significantly higher p53 immunoexpression than non-anaplastic. The preoperative chemotherapy did not modify p53 immunoexpression of WT which had been found in other similar studies.
Djuricić
Slavisa
S
Djokić
Dragomir
D
Vujić
Dragana
D
Basta-Jovanović
Gordana
G
Todorović
Vera
V
Radojević-Skodrić
Sanja
S
Zdravković
Slavisa
S
Vujanić
Gordan M
GM
D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007223:Infant; D007668:Kidney / Q000473:pathology; D007680:Kidney Neoplasms / Q000378:metabolism*; D008297:Male; D011379:Prognosis; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*; D009396:Wilms Tumor / Q000378:metabolism* / Q000473:pathology
D016159:Tumor Suppressor Protein p53