title: Applications of heparin and heparan sulfate microarrays.
abstract: Carbohydrate microarrays have become crucial tools for revealing the biological interactions and functions of glycans, primarily because the microarray format enables the investigation of large numbers of carbohydrates at a time. Heparan sulfate (HS) and heparin are the most structurally complex glycosaminoglycans (GAGs). In this chapter, we describe the preparation of a small library of HS/heparin oligosaccharides, and the fabrication of HS/heparin microarrays that have been used to establish HS/heparin-binding profiles. Fibroblast growth factors (FGFs), natural cytotoxicity receptors (NCRs), and chemokines were screened to illuminate the very important biological functions of these glycans.
authors.affiliation: Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, Research Campus Potsdam-Golm, Potsdam, Germany.
authors.forename: Jian
authors.initials: J
authors.lastname: Yin
authors.forename: Peter H
authors.initials: PH
authors.lastname: Seeberger
mesh_terms: D000818:Animals; D002240:Carbohydrate Sequence; D006493:Heparin / Q000737:chemistry*; D006497:Heparan Sulfate / Q000737:chemistry*; D006801:Humans; D046228:Microarray Analysis / Q000295:instrumentation / Q000379:methods; D008969:Molecular Sequence Data
chemical_list: D006493:Heparin; D006497:Heparan Sulfate