title: Spatial-domain low-coherence quantitative phase microscopy for cancer diagnosis.
abstract: A microscopy technique, spatial-domain low-coherence quantitative phase microscopy (SL-QPM) is proposed for speckle-free, quantitative phase imaging of subcellular structures with subnanometer sensitivity. We quantified, for the first time to our knowledge, the refractive index of the cell nuclei on original unmodified histology specimens. We demonstrate that the refractive index of cell nucleus is highly sensitive in detecting cancer, especially in histologically normal-appearing cells from cancer patients. Because this technique is sensitive and does not require special sample processing, it can be disseminated to all clinical settings.
authors.affiliation: Department of Medicine, Division of Gastroenterology, Hepatology and Nutrition, University of Pittsburgh, Pittsburgh, Pennsylvania 15232, USA.
authors.forename: Pin
authors.initials: P
authors.lastname: Wang
authors.forename: Rajan
authors.initials: R
authors.lastname: Bista
authors.forename: Rohit
authors.initials: R
authors.lastname: Bhargava
authors.forename: Randall E
authors.initials: RE
authors.lastname: Brand
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
mesh_terms: D006801:Humans; D042541:Intracellular Space / Q000378:metabolism; D008853:Microscopy / Q000379:methods*; D036103:Nanotechnology; D009369:Neoplasms / Q000175:diagnosis* / Q000473:pathology; D055620:Optical Phenomena; D015203:Reproducibility of Results; D012815:Signal Processing, Computer-Assisted
references.citation: Opt Lett. 2005 Aug 15;30(16):2131-3
references.pmid: 16127933
references.citation: Opt Lett. 2006 May 15;31(10):1462-4
references.pmid: 16642139
references.citation: Opt Lett. 2005 Sep 15;30(18):2445-7
references.pmid: 16196347
references.citation: J Opt Soc Am A Opt Image Sci Vis. 2001 Feb;18(2):319-25
references.pmid: 11205977
references.citation: Opt Lett. 2003 Jul 15;28(14):1230-2
references.pmid: 12885030
references.citation: Opt Lett. 2004 Sep 1;29(17):2028-30
references.pmid: 15455769
references.citation: Opt Lett. 2008 Sep 15;33(18):2074-6
references.pmid: 18794935