[Protein analysis of Trichomonas vaginalis treated by Pulsatilla chinensis in vitro].
Water extract of Pulsatilla chinensis (PWE) (0.625 mg/ml) was added to the medium for the culture of Trichomonas vaginalis. After 2 h and 4 h treatment, the content and component of soluble proteins in the parasites were analyzed by SDS-PAGE. A total of 28 protein bands was demonstrated. After treated with PWE for 2 h, the content of 5 protein bands increased and that of 4 bands decreased significantly. Four hours later, a new protein band appeared in the electrophoretogram, and the content of 5 bands increased and that of other 5 bands decreased.
Yan
Yan
Y
Department of Pathgenic Biology, Baotou Medical College, Baotou 014010, China.
Gao
Xing-Zheng
XZ
D000953:Antigens, Protozoan / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D015800:Protozoan Proteins / Q000378:metabolism*; D031946:Pulsatilla / Q000737:chemistry*; D014246:Trichomonas vaginalis / Q000187:drug effects*
D000953:Antigens, Protozoan; D010936:Plant Extracts; D015800:Protozoan Proteins