title: Resolving postglacial phylogeography using high-throughput sequencing.
abstract: The distinction between model and nonmodel organisms is becoming increasingly blurred. High-throughput, second-generation sequencing approaches are being applied to organisms based on their interesting ecological, physiological, developmental, or evolutionary properties and not on the depth of genetic information available for them. Here, we illustrate this point using a low-cost, efficient technique to determine the fine-scale phylogenetic relationships among recently diverged populations in a species. This application of restriction site-associated DNA tags (RAD tags) reveals previously unresolved genetic structure and direction of evolution in the pitcher plant mosquito, Wyeomyia smithii, from a southern Appalachian Mountain refugium following recession of the Laurentide Ice Sheet at 22,000-19,000 B.P. The RAD tag method can be used to identify detailed patterns of phylogeography in any organism regardless of existing genomic data, and, more broadly, to identify incipient speciation and genome-wide variation in natural populations in general.
authors.affiliation: Center for Ecology and Evolutionary Biology, University of Oregon, Eugene, OR 97403-5289, USA.
authors.forename: Kevin J
authors.initials: KJ
authors.lastname: Emerson
authors.forename: Clayton R
authors.initials: CR
authors.lastname: Merz
authors.forename: Julian M
authors.initials: JM
authors.lastname: Catchen
authors.forename: Paul A
authors.initials: PA
authors.lastname: Hohenlohe
authors.forename: William A
authors.initials: WA
authors.lastname: Cresko
authors.forename: William E
authors.initials: WE
authors.lastname: Bradshaw
authors.forename: Christina M
authors.initials: CM
authors.lastname: Holzapfel
mesh_terms: D000818:Animals; D001061:Appalachian Region; D001483:Base Sequence; D009033:Culicidae / Q000737:chemistry / Q000235:genetics*; D005828:Genetics, Population; D008873:Mid-Atlantic Region; D008969:Molecular Sequence Data; D009511:New England; D010802:Phylogeny; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA / Q000379:methods*
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