title: O-GlcNAc transferase regulates mitotic chromatin dynamics.
abstract: Mitosis must faithfully divide the genome such that each progeny inherits the same genetic material. DNA condensation is crucial in ensuring that chromosomes are correctly attached to the mitotic spindle for segregation, preventing DNA breaks or constrictions from the contractile ring. Histones form an octameric complex of basic proteins important in regulating DNA organization and accessibility. Histone post-translational modifications are altered during mitosis, although the roles of these post-translational modifications remain poorly characterized. Here, we report that N-acetylglucosamine (O-GlcNAc) transferase (OGT), the enzyme catalyzing the addition of O-GlcNAc moieties to nuclear and cytoplasmic proteins at serine and threonine residues, regulates some aspects of mitotic chromatin dynamics. OGT protein amounts decrease during M phase. Modest overexpression of OGT alters mitotic histone post-translational modifications at Lys-9, Ser-10, Arg-17, and Lys-27 of histone H3. Overexpression of OGT also prevents mitotic phosphorylation of coactivator-associated arginine methyltransferase 1 (CARM1) and prevents its correct cellular localization during mitosis. Moreover, OGT overexpression results in an increase in abnormal chromosomal bridge formation. Together, these results show that regulating the amount of OGT during mitosis is important in ensuring correct chromosomal segregation during mitosis.
authors.affiliation: Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.
authors.forename: Kaoru
authors.initials: K
authors.lastname: Sakabe
authors.forename: Gerald W
authors.initials: GW
authors.lastname: Hart
mesh_terms: D002843:Chromatin / Q000201:enzymology* / Q000235:genetics; D042002:Chromatin Assembly and Disassembly / Q000502:physiology*; D020090:Chromosome Segregation / Q000502:physiology*; D002877:Chromosomes, Human / Q000235:genetics / Q000378:metabolism; D053960:DNA Breaks; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology; D015894:Genome, Human / Q000502:physiology; D006367:HeLa Cells; D006657:Histones / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008938:Mitosis / Q000502:physiology*; D017351:N-Acetylglucosaminyltransferases / Q000235:genetics / Q000378:metabolism*; D011499:Protein Processing, Post-Translational / Q000502:physiology*; D021381:Protein Transport / Q000502:physiology; D011484:Protein-Arginine N-Methyltransferases; D008941:Spindle Apparatus / Q000201:enzymology / Q000235:genetics
chemical_list: D002843:Chromatin; D006657:Histones; D011484:Protein-Arginine N-Methyltransferases; C119966:coactivator-associated arginine methyltransferase 1; D017351:N-Acetylglucosaminyltransferases
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