title: Perturbation of indole-3-butyric acid homeostasis by the UDP-glucosyltransferase UGT74E2 modulates Arabidopsis architecture and water stress tolerance.
abstract: Reactive oxygen species and redox signaling undergo synergistic and antagonistic interactions with phytohormones to regulate protective responses of plants against biotic and abiotic stresses. However, molecular insight into the nature of this crosstalk remains scarce. We demonstrate that the hydrogen peroxide-responsive UDP-glucosyltransferase UGT74E2 of Arabidopsis thaliana is involved in the modulation of plant architecture and water stress response through its activity toward the auxin indole-3-butyric acid (IBA). Biochemical characterization of recombinant UGT74E2 demonstrated that it strongly favors IBA as a substrate. Assessment of indole-3-acetic acid (IAA), IBA, and their conjugates in transgenic plants ectopically expressing UGT74E2 indicated that the catalytic specificity was maintained in planta. In these transgenic plants, not only were IBA-Glc concentrations increased, but also free IBA levels were elevated and the conjugated IAA pattern was modified. This perturbed IBA and IAA homeostasis was associated with architectural changes, including increased shoot branching and altered rosette shape, and resulted in significantly improved survival during drought and salt stress treatments. Hence, our results reveal that IBA and IBA-Glc are important regulators of morphological and physiological stress adaptation mechanisms and provide molecular evidence for the interplay between hydrogen peroxide and auxin homeostasis through the action of an IBA UGT.
authors.affiliation: Department of Plant Systems Biology, VIB, Ghent University, 9052 Gent, Belgium.
authors.forename: Vanesa B
authors.initials: VB
authors.lastname: Tognetti
authors.forename: Olivier
authors.initials: O
authors.lastname: Van Aken
authors.forename: Kris
authors.initials: K
authors.lastname: Morreel
authors.forename: Korneel
authors.initials: K
authors.lastname: Vandenbroucke
authors.forename: Brigitte
authors.initials: B
authors.lastname: van de Cotte
authors.forename: Inge
authors.initials: I
authors.lastname: De Clercq
authors.forename: Sheila
authors.initials: S
authors.lastname: Chiwocha
authors.forename: Ricarda
authors.initials: R
authors.lastname: Fenske
authors.forename: Els
authors.initials: E
authors.lastname: Prinsen
authors.forename: Wout
authors.initials: W
authors.lastname: Boerjan
authors.forename: Bernard
authors.initials: B
authors.lastname: Genty
authors.forename: Keith A
authors.initials: KA
authors.lastname: Stubbs
authors.forename: Dirk
authors.initials: D
authors.lastname: Inzé
authors.forename: Frank
authors.initials: F
authors.lastname: Van Breusegem
mesh_terms: D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics / Q000254:growth & development*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D003681:Dehydration; D005964:Glucosyltransferases / Q000235:genetics / Q000378:metabolism*; D006706:Homeostasis; D007210:Indoleacetic Acids / Q000378:metabolism; D007211:Indoles / Q000378:metabolism*; D016254:Mutagenesis, Insertional; D030821:Plants, Genetically Modified / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D013312:Stress, Physiological
chemical_list: D029681:Arabidopsis Proteins; D007210:Indoleacetic Acids; D007211:Indoles; C014612:indolebutyric acid; C030737:indoleacetic acid; D005964:Glucosyltransferases
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