title: Rapid ultraperformance liquid chromatography-tandem mass spectrometry method for quantification of oxcarbazepine and its metabolite in human plasma.
abstract: A rapid and sensitive ultraperformance liquid chromatography tandem mass spectrometry assay was developed for the simultaneous analysis of oxcarbazepine and its main metabolite in human plasma. The assay involves a simple solid-phase extraction procedure of 0.3 mL of human plasma and analysis was performed on a triple-quadrupole tandem mass spectrometer in multiple reaction monitoring mode via electrospray ionization. Separation was achieved on an Acquity UPLC™ BEH C₁₈ column (50 × 2.1 mm, i.d., 1.7 µm) with isocratic elution at a flow-rate of 0.25 mL/min and imipramine was used as the internal standard. The standard calibration curve was linear over the range 9.580-5070.205 ng/mL for oxcarbazepine (OXC) and 19.444-10290.800 ng/mL for 10,11-dihydro-10-hydroxycarbamazepine (MHD), expressed by the linear correlation coefficient r², which was better than 0.995 for OXC and MHD. The intra- and inter-day precision and accuracy of the quality control samples were within 10.0%. The recoveries were 81.0, 89.6 and 66.6% for OXC, MHD and imipramine, respectively. The total run time was 1.5 min only for each sample, which makes it possible to analyze more than 350 samples per day.
authors.affiliation: Synchron Research Services Private Limited, The Chambers, 5th Floor, S-G Highway, Ahmedabad, India. mitu_070980@yahoo.co.in
authors.forename: Mitesh
authors.initials: M
authors.lastname: Bhatt
authors.forename: Sanjay
authors.initials: S
authors.lastname: Shah
authors.lastname: Shivprakash
mesh_terms: D002220:Carbamazepine / Q000031:analogs & derivatives* / Q000097:blood; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D006801:Humans; D016018:Least-Squares Analysis; D000078330:Oxcarbazepine; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013997:Time Factors
chemical_list: C039775:10,11-dihydro-10-hydroxycarbamazepine; D002220:Carbamazepine; D000078330:Oxcarbazepine