title: Analytical aspects of cyanobacterial volatile organic compounds for investigation of their production behavior.
abstract: In order to fully understand the role of volatile organic compounds (VOCs) under natural conditions, an adaptable analytical method was developed as the first step. beta-Ionone, beta-cyclocitral, 2-methyl-1-butanol and 3-methyl-1-butanol were simultaneously analyzed in addition to geosmin and 2-MIB using GC/MS with SPME. The slight modification of a known method allowed the simultaneous detection and quantification of these VOCs. The SIM of the 3-methyl-1-butanol was always accompanied by a shoulder peak, suggesting the presence of two compounds. In order to separate both compounds, the GC/MS conditions were optimized, and the additional peak was identified as 2-methyl-1-butanol by direct comparison of the authentic compound, indicating that the Microcystis strain always produces a mixture of 2-methyl-1-butanol and 3-methyl-1-butanol. Furthermore, it was found that 2-methyl-1-butanol and 3-methyl-1-butanol were predominant in the dissolved fractions. beta-Cyclocitral was easily oxidized to provide the oxidation product, 2,6,6-trimethylcyclohexene-1-carboxylic acid, which causes the blue color formation of cyanobacteria as a consequence of acid stress. The intact acid could be satisfactorily analyzed using the usual GC/MS without derivatization.
authors.affiliation: Graduate School of Environmental and Human Science and Faculty of Pharmacy, Meijo University, 150 Yagotoyama, Tempaku, Nagoya 468-8503, Japan. fujise-d@city.kawasaki.jp
authors.forename: Daiki
authors.initials: D
authors.lastname: Fujise
authors.forename: Kiyomi
authors.initials: K
authors.lastname: Tsuji
authors.forename: Naoko
authors.initials: N
authors.lastname: Fukushima
authors.forename: Kohei
authors.initials: K
authors.lastname: Kawai
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Harada
mesh_terms: D000440:Butanols / Q000737:chemistry; D000458:Cyanobacteria / Q000737:chemistry*; D005618:Fresh Water / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D052617:Solid Phase Microextraction / Q000379:methods*; D055549:Volatile Organic Compounds / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D000440:Butanols; D055549:Volatile Organic Compounds