title: Assessment of polyphenolic compounds, in vitro antioxidant and anti-inflammation properties of Securidaca longepedunculata root barks.
abstract: The present study was carried out to evaluate the antioxidant activities of the root bark extract of Securidaca longepedunculata. This plant material is commonly used in folk medicine in several parts in the world. The bark extracts of S. longepedunculata were evaluated for their total phenols, flavonoids, anthocyanins, tannins content and total antioxidant capacity. The compounds were identified and quantified both by RP-HPLC and UV spectrophotometer; the antioxidant capacity was assessed by ABTS and DPPH tests and expressed as IC(50). The total phenolic compounds determinate was 9.86mg gallic acid equivalents/g dw, the total flavonoid contents was 5.85mg catechin equivalents/g dw, the total anthocyanin contents was 0.032mg cyanidin-3-glycosyl equivalents/g dw and the condensed tannins content were 1.03mg catechin equivalents/g dw. The major compound identified using RP-HPLC was quercetin (0.98mg/ml). The IC(50) value reached 5.5microg/ml, revealing that the root barks of S. longepedunculata have a very high antioxidant and anti-inflammation properties.
authors.affiliation: Chemistry Laboratory and methodology for Environment, Paul-Verlaine University, boulevard Arago, Metz, France. franckmuanda@gmail.com
authors.forename: François Nsemi
authors.initials: FN
authors.lastname: Muanda
authors.forename: Amadou
authors.initials: A
authors.lastname: Dicko
authors.forename: Rachid
authors.initials: R
authors.lastname: Soulimani
mesh_terms: D000818:Animals; D000872:Anthocyanins / Q000032:analysis; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000302:isolation & purification / Q000494:pharmacology*; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D052160:Benzothiazoles; D001713:Biphenyl Compounds; D002851:Chromatography, High Pressure Liquid; D056148:Chromatography, Reverse-Phase; D003124:Colorimetry; D004353:Drug Evaluation, Preclinical; D007887:Fabaceae / Q000737:chemistry*; D005419:Flavonoids / Q000032:analysis / Q000302:isolation & purification / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000302:isolation & purification / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D009549:Nigeria; D009569:Nitric Oxide / Q000378:metabolism; D010636:Phenols / Q000032:analysis / Q000302:isolation & purification / Q000494:pharmacology*; D010851:Picrates; D024301:Plant Bark / Q000737:chemistry*; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry*; D059808:Polyphenols; D044945:Proanthocyanidins / Q000032:analysis; D013451:Sulfonic Acids; D013844:Thiazoles
chemical_list: D000872:Anthocyanins; D000894:Anti-Inflammatory Agents, Non-Steroidal; D000975:Antioxidants; D052160:Benzothiazoles; D001713:Biphenyl Compounds; D005419:Flavonoids; D016166:Free Radical Scavengers; D010636:Phenols; D010851:Picrates; D010936:Plant Extracts; D059808:Polyphenols; D044945:Proanthocyanidins; D013451:Sulfonic Acids; D013844:Thiazoles; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D009569:Nitric Oxide; C004931:1,1-diphenyl-2-picrylhydrazyl