Interleukin-18 deficiency reduces neuropeptide gene expressions in the mouse amygdala related with behavioral change.
In this study, we examined the effects of IL-18 deficiency on behaviors and gene expression profiles in 6 brain regions. IL-18(-/-) mice reduced depressive-like behavior and changed gene expressions predominantly in the amygdala compared with wild-type mice. Pathway analysis of the differentially expressed genes ranked behavior as the top-scored biological function. Of note, the absence of IL-18 decreased Avp, Hcrt, Oxt, and Pmch mRNA levels and the number of arginine vasopressin- and oxytocin-positive cells in the amygdala, but not in the hypothalamus. Our results suggest that IL-18-dependent vasopressinergic and oxytocinergic circuitry in the amygdala may regulate depressive-like behaviors in mice.
Yamamoto
Yuta
Y
Departments of Stress Science, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima, Japan.
Tanahashi
Toshihito
T
Katsuura
Sakurako
S
Kurokawa
Ken
K
Nishida
Kensei
K
Kuwano
Yuki
Y
Kawai
Tomoko
T
Teshima-Kondo
Shigetada
S
Chikahisa
Sachiko
S
Tsuruo
Yoshihiro
Y
Sei
Hiroyoshi
H
Rokutan
Kazuhito
K
D000222:Adaptation, Physiological / Q000235:genetics; D054366:Agouti Signaling Protein / Q000235:genetics / Q000378:metabolism; D000679:Amygdala / Q000378:metabolism*; D000704:Analysis of Variance; D000818:Animals; D001522:Behavior, Animal; D019295:Computational Biology / Q000379:methods; D005106:Exploratory Behavior / Q000502:physiology; D020869:Gene Expression Profiling / Q000379:methods; D005786:Gene Expression Regulation / Q000235:genetics*; D007028:Hypothalamic Hormones / Q000235:genetics / Q000378:metabolism; D020382:Interleukin-18 / Q000172:deficiency*; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism; D008297:Male; D018782:Maze Learning / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009479:Neuropeptides / Q000235:genetics / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D000068797:Orexins; D010121:Oxytocin / Q000235:genetics / Q000378:metabolism; D011498:Protein Precursors / Q000235:genetics / Q000378:metabolism; D013550:Swimming / Q000523:psychology; D014667:Vasopressins / Q000235:genetics / Q000378:metabolism
D054366:Agouti Signaling Protein; D007028:Hypothalamic Hormones; D020382:Interleukin-18; D047908:Intracellular Signaling Peptides and Proteins; D009479:Neuropeptides; D000068797:Orexins; D011498:Protein Precursors; C515183:a protein, mouse; D014667:Vasopressins; C059991:melanin-concentrating hormone precursors; D010121:Oxytocin