Antiproliferative activity of N6-isopentenyladenosine on MCF-7 breast cancer cells: cell cycle analysis and DNA-binding study.
N(6)-Isopentenyladenosine (iPA) is a member of the cytokinins, a family of plant hormones that regulate plant cell growth and differentiation. iPA is present in mammalian cells in a free form, as a mononucleotide in the cytoplasm, or in a tRNA-bound form. Its antiproliferative activity against cancer cells lines has been reported by several authors. We show that iPA inhibits the growth of human breast cancer MCF-7 cells in a dose-dependent manner with a concentration of 12.2 μM, which causes 50% inhibition of cell viability. The cell cycle analysis by flow cytometry showed that iPA-induced growth arrest could be associated to apoptosis. The interaction of DNA with iPA was studied in aqueous solution at physiological conditions by ultraviolet-vis spectroscopy. A binding constant K(iPA-DNA) = 4.4 × 10(3) M(-1) was calculated and this value, together with the shift of absorbance, suggests iPA-DNA interaction at DNA surface.
Rajabi
Mehdi
M
Laboratory of Biochemistry and Molecular Biology, Department of Medicine, Surgery and Dentistry, Polo Universitario S. Paolo, Università degli Studi di Milano, Milano, Italy.
Signorelli
Paola
P
Gorincioi
Elena
E
Ghidoni
Riccardo
R
Santaniello
Enzo
E
D000042:Absorption; D017209:Apoptosis / Q000187:drug effects; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology*; D002453:Cell Cycle / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004247:DNA / Q000378:metabolism*; D006801:Humans; D007541:Isopentenyladenosine / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*; D013057:Spectrum Analysis; D013237:Stereoisomerism
D007541:Isopentenyladenosine; D004247:DNA