[Effect of GM6001 on the model that cervical squamous carcinoma cell line HCE1 multicellular spheroids invade live human umbilical vein endothelium cell monolayers].
OBJECTIVE
To investigate the relationship between matrix matrix metalloproteinases (MMP)-9 expression in HCE1/multicellular spheroids (MCS) and invasion of cervical carcinoma, and to explore the effect of MMPs inhibitor GM6001 on the model that HCE1/MCS invade live human umbilical vein endothelium cell (HUVEC).


METHODS
We established the invasion model by coculturing HCE1/MCS and HUVEC, and detected the MMP-9 expression in HCE1 monolayer cells (HCE1/MC), HCE1/MCS and HUVEC using immunohistochemistry and treated the HCE1/MCS invasion model with GM6001.


RESULTS
A cervical squamous carcinoma cell HCE1 infiltrating model was established. Compared with that in HCE1/MC, MMP-9 expression in the HCE1/MCS was significantly higher (P<0.05). Compared with the control group, the invasion of HCE1/MCS was inhibited by 26.09% and 92.95% (P<0.05) in the GM6001 2.5 μmol/L and 12.5 μmol/L group. MMP-9 expression in HCE1/MCS in the GM6001 12.5 μmol/L group was obviously lower than that in the control group (P<0.05). With the increase of the concentration of GM6001, the inhibition increased.


CONCLUSIONS
The enhancement of HCE1/MCS invasion may be related to the up-regulation of MMP-9 expression in HCE1/MCS. GM6001 can down regulate the MMP-9 expression in HCE1/MCS and partially block the HCE1/MCS invasion to HUVEC.
Lan
Jing
J
Department of Gynecology and Obstetrics, Second Xiangya Hospital, Central South University, Changsha 410011, China.
Wu
Yilin
Y
D002294:Carcinoma, Squamous Cell / Q000473:pathology; D002460:Cell Line; D045744:Cell Line, Tumor; D004151:Dipeptides / Q000494:pharmacology*; D042783:Endothelial Cells / Q000473:pathology*; D005260:Female; D006801:Humans; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D018874:Spheroids, Cellular / Q000378:metabolism* / Q000473:pathology; D014471:Umbilical Veins / Q000166:cytology; D002583:Uterine Cervical Neoplasms / Q000473:pathology*
D004151:Dipeptides; C078131:N-(2(R)-2-(hydroxamidocarbonylmethyl)-4-methylpentanoyl)-L-tryptophan methylamide; D020780:Matrix Metalloproteinase 9