Specificity of DNA sequences recognized by the zinc-finger homeodomain protein, GmZF-HD1 in soybean.
Zinc finger-homeodomain proteins (ZF-HDs) have been identified in many plant species. In soybean (Glycine max), GmZF-HD1 functions as a transcription factor that activates the soybean calmodulin isoform-4 (GmCaM-4) gene in response to pathogens. Recently, we reported specific binding of GmZF-HD1 to a 30-nt A/T-rich cis-element which constitutes two repeats of a conserved homeodomain binding site, ATTA, within -1207 to -1128bp of the GmCaM-4 promoter. Herein, homeodomain sequences of the GmZF-HD1 protein were compared to those of other homeodomain proteins and characterized the specificity of DNA sequences in the interaction of the GmCaM-4 promoter with GmZF-HD1 protein. Considering the conservation of homeodomains in plants, the AG sequence within a 30-nt A/T-rich cis-element is required for binding of the GmZF-HD1 protein. Approximately 25-bp of A/T-rich DNA sequences containing an AG sequence is necessary for effective binding to the GmZF-HD1 protein. Taken together, the results support the notion that the GmZF-HD1 protein specifically functions in plant stress signalling by interacting with the promoter of GmCaM-4.
Park
Hyeong Cheol
HC
Division of Applied Life Science (BK21 program), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju 660-701, Republic of Korea. hcpark@gnu.ac.kr
Kim
Man Lyang
ML
Kim
Ho Soo
HS
Park
Jung Hoon
JH
Jung
Mi Soon
MS
Shen
Mingzhe
M
Kang
Chang Ho
CH
Kim
Min Chul
MC
Lee
Sang Yeol
SY
Cho
Moo Je
MJ
Chung
Woo Sik
WS
Yun
Dae-Jin
DJ
D001483:Base Sequence; D002147:Calmodulin / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D018398:Homeodomain Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D020033:Protein Isoforms; D030262:Soybean Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D013025:Glycine max / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D016335:Zinc Fingers / Q000235:genetics*
D002147:Calmodulin; D004268:DNA-Binding Proteins; D018398:Homeodomain Proteins; D020033:Protein Isoforms; D030262:Soybean Proteins; D014157:Transcription Factors