title: Hypopigmenting activity of bisabolangelone isolated from Angelica koreana Maxim. in α-melanocyte stimulating hormone-activated B16 or melan-a cells.
abstract: Tyrosinase is a key enzyme in the biosynthetic pathway of melanin pigments. Abnormal accumulation of melanin pigments causes melasma, freckles, and senile lentigo, which can be substantially ameliorated by treatment with arbutin or other tyrosinase inhibitors. In this study, roots of Angelica koreana Maxim. (Umbelliferae) inhibited melanin production in α-melanocyte stimulating hormone ( α-MSH)-activated B16 melanoma cells or melan-a melanocytes. To elucidate the hypopigmenting principle of A. koreana, the plant extracts were subjected to bioassay-guided phytochemical analysis, resulting in the identification of bisabolangelone. Bisabolangelone dose-dependently inhibited α-MSH-induced melanin production in B16 or melan-a cells with IC(15) values of 9-17 µM. The positive control arbutin also inhibited melanin production in B16 cells with an IC(50) value of 317 µM. Bisabolangelone suppressed α-MSH-inducible protein levels of tyrosinase in B16 cells but could not significantly inhibit the catalytic activity of cell-free tyrosinase. Taken together, this study indicates that bisabolangelone is the primary hypopigmenting principle of A. koreana and may have pharmacological potential in the melanin-associated hyperpigmentation disorders.
authors.affiliation: College of Pharmacy, Chungbuk National University, Cheongju, Republic of Korea.
authors.forename: Eunmiri
authors.initials: E
authors.lastname: Roh
authors.forename: Cheong-Yong
authors.initials: CY
authors.lastname: Yun
authors.forename: Jin Woo
authors.initials: JW
authors.lastname: Lee
authors.forename: Chul
authors.initials: C
authors.lastname: Lee
authors.forename: Bang Yeon
authors.initials: BY
authors.lastname: Hwang
authors.forename: Sun Tack
authors.initials: ST
authors.lastname: Oh
authors.forename: Sang-Hun
authors.initials: SH
authors.lastname: Jung
authors.forename: Sang-Bae
authors.initials: SB
authors.lastname: Han
authors.forename: Youngsoo
authors.initials: Y
authors.lastname: Kim
mesh_terms: D029969:Angelica / Q000737:chemistry*; D000818:Animals; D002460:Cell Line; D045744:Cell Line, Tumor; D003879:Dermatologic Agents / Q000494:pharmacology / Q000627:therapeutic use; D004305:Dose-Response Relationship, Drug; D017495:Hyperpigmentation / Q000188:drug therapy* / Q000378:metabolism; D020128:Inhibitory Concentration 50; D058965:MART-1 Antigen / Q000378:metabolism; D008543:Melanins / Q000096:biosynthesis*; D008544:Melanocytes / Q000187:drug effects; D008546:Melanoma, Experimental / Q000188:drug therapy; D014442:Monophenol Monooxygenase / Q000378:metabolism*; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D018517:Plant Roots; D012717:Sesquiterpenes / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D012867:Skin / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D000521:alpha-MSH / Q000378:metabolism
chemical_list: D003879:Dermatologic Agents; D058965:MART-1 Antigen; D008543:Melanins; D010936:Plant Extracts; D012717:Sesquiterpenes; C455067:bisabolangelone; D000521:alpha-MSH; D014442:Monophenol Monooxygenase