Negative regulation of stress-induced matrix metalloproteinase-9 by Sirt1 in skin tissue.
Solar ultra-violet (UV) radiation and the ensuing photo-damage are adverse factors affecting human skin directly exposed to the sun. Stress responses induced by UV radiation (UVR) elicit premature skin ageing (photoageing), resulting in extensive damage to dermal connective tissue. Disruption of the normal dermal structure of skin connective tissue, primarily collagen, impairs a variety of skin functions and is considered to be the main cause of wrinkle formation. Matrix metalloproteases (MMPs) may be responsible for the degradation of collagen and other extracellular matrix proteins, which are major targets for relieving skin photoageing. Herein, we demonstrated that Sirt1, a putative anti-ageing enzyme, reduced MMP-9 transcriptional expression in skin. The known agonists of Sirt1, resveratrol and metformin, also significantly inhibited MMP-9 expression and appeared to protect collagen from degradation after UVR. These studies suggest that the Sirt1 activator could be used as a novel therapeutic agent to delay skin photoageing.
Lee
Ji-Seon
JS
CUSKIN Cosmeceutical & Biotechnology, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine, Seoul, Korea.
Park
Keung-Young
KY
Min
Hyung-Geun
HG
Lee
Seung Jae
SJ
Kim
Jin-Ju
JJ
Choi
Joon-Seok
JS
Kim
Won-Serk
WS
Cha
Hyuk-Jin
HJ
D000818:Animals; D055162:Biocatalysis / Q000187:drug effects / Q000528:radiation effects; D002478:Cells, Cultured; D024141:Collagen Type IV / Q000378:metabolism; D020405:Dermis / Q000166:cytology; D005260:Female; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D015870:Gene Expression / Q000187:drug effects / Q000235:genetics / Q000528:radiation effects; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism*; D008687:Metformin / Q000494:pharmacology; D051379:Mice; D008812:Mice, Hairless; D000077185:Resveratrol; D056564:Sirtuin 1 / Q000037:antagonists & inhibitors / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D012867:Skin / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D013267:Stilbenes / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014466:Ultraviolet Rays
D024141:Collagen Type IV; D013267:Stilbenes; D008687:Metformin; D020780:Matrix Metalloproteinase 9; C501830:Mmp9 protein, mouse; C447939:SIRT1 protein, human; C470545:Sirt1 protein, mouse; D056564:Sirtuin 1; D013755:Tetradecanoylphorbol Acetate; D000077185:Resveratrol