title: Small-scale extraction of recombinant proteins from bacteria.
abstract: Bacteria are particularly convenient for producing recombinant proteins for purification purposes. To monitor induction as well as the levels of recombinant protein expression, it is important to have a rapid, simple method for estimating bacterial protein expression. This protocol describes the preparation of small-scale bacterial extracts using cell lysis with 0.5% Triton X-100.
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Simpson
mesh_terms: D001419:Bacteria / Q000235:genetics / Q000378:metabolism*; D001433:Bacteriolysis; D015870:Gene Expression; D015202:Protein Engineering / Q000379:methods; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D011994:Recombinant Proteins