Magnetic resonance studies of macromolecular content in engineered cartilage treated with pulsed low-intensity ultrasound.
Noninvasive monitoring of matrix development in tissue-engineered cartilage constructs would permit ongoing assessment with the ability to modify culture conditions during development to optimize tissue characteristics. In this study, chondrocytes seeded in a collagen hydrogel were exposed for 20 min/day to pulsed low-intensity ultrasound (PLIUS) at 30 mWcm(-2) and cultured for up to 5 weeks. Biochemical assays, histology, immunohistochemistry, Fourier transform infrared spectroscopy, and magnetic resonance imaging (MRI) were performed at weeks 3 and 5 after initiation of growth. The noninvasive MRI measurements were correlated with those from the invasive studies. In particular, MRI transverse relaxation time (T2) and magnetization transfer rate (k(m)) correlated with macromolecular content, which was increased by application of PLIUS. This indicates the sensitivity of MR techniques to PLIUS-induced changes in matrix development, and highlights the potential for noninvasive assessment of the efficacy of anabolic interventions for engineered tissue.
Irrechukwu
Onyi N
ON
Magnetic Resonance Imaging and Spectroscopy Section, Gerontology Research Center 4D-06, National Institute on Aging, National Institutes of Health, Baltimore, Maryland, USA.
Lin
Ping-Chang
PC
Fritton
Kate
K
Doty
Steve
S
Pleshko
Nancy
N
Spencer
Richard G
RG
D000818:Animals; D002358:Cartilage, Articular / Q000166:cytology / Q000502:physiology* / Q000528:radiation effects; D002417:Cattle; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology / Q000502:physiology* / Q000528:radiation effects; D020219:Chondrogenesis / Q000502:physiology* / Q000528:radiation effects; D006025:Glycosaminoglycans / Q000378:metabolism*; D046911:Macromolecular Substances / Q000378:metabolism; D008279:Magnetic Resonance Imaging / Q000379:methods*; D013010:Sonication / Q000379:methods*; D014018:Tissue Distribution; D023822:Tissue Engineering / Q000379:methods*
D006025:Glycosaminoglycans; D046911:Macromolecular Substances
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