Uniaxial cyclic tensile stretch inhibits osteogenic and odontogenic differentiation of human dental pulp stem cells.
As the most important organs of occlusion, teeth are subjected to a variety of mechanical stresses. These stresses are transmitted into the dental pulp tissue and affect the dental pulp stem cells. In this study, human dental pulp stem cells were isolated from human impacted third molars and their multilineage differentiation abilities were tested. Human dental pulp stem cells were then exposed to cyclic tensile stretch. The results showed that the cyclic tensile stretch inhibited the expression of osteogenic marker genes and proteins such as BMP-2, OCN and ALP. Simultaneously, odontogenic marker genes and proteins such as DSPP, DSP and BSP were also inhibited by the mechanical stress. It was concluded that cyclic tensile stretch inhibits the osteogenic and odontogenic differentiation of dental pulp stem cells.
Cai
Xiaoxiao
X
State Key Laboratory of Oral Diseases, West China College of Stomatology, Sichuan University, Chengdu, People's Republic of China.
Zhang
Yi
Y
Yang
Xingmei
X
Grottkau
Brian E
BE
Lin
Yunfeng
Y
D017667:Adipocytes / Q000166:cytology; D000293:Adolescent; D000328:Adult; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D019070:Cell Lineage; D003470:Culture Media; D003782:Dental Pulp / Q000166:cytology*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods; D009805:Odontogenesis; D010012:Osteogenesis; D013234:Stem Cells / Q000166:cytology*; D013314:Stress, Mechanical; D013718:Tensile Strength; D023822:Tissue Engineering
D003470:Culture Media