Expression and localization of prominin-1 in isoproterenol-treated rat parotid gland.
Prominin-1 (CD133) is a pentaspan cholesterol-binding membrane glycoprotein. Chronic treatment with isoproterenol, a beta-receptor agonist, induces several dramatic effects on salivary glands, such as enhanced DNA synthesis and proliferation of salivary acinar cells. In addition, the biosynthetic pathways of membrane lipids may be altered by the isoproterenol stimulation. The purpose of this study was to investigate the effect of isoproterenol administration on prominin-1 expression profiles in rat parotid gland by means of PCR and immunohistochemistry. Rats were chronically treated with the beta-adrenergic agonist for 1, 3, and 7 days. Our results showed that isoproterenol-treatment caused a down-regulation of prominin-1 on day 3 and 7 of treatment, as well as a differential immunostaining distribution pattern. This study suggests that isoproterenol-treatment may represent a useful tool to explore the molecular mechanisms involved in the synthesis and release of prominin-1. Such efforts could contribute to the development of diagnostic tools based on the detection of prominin-1 in biological fluids, such as saliva.
D'Amico
Fabio
F
Department of Biomedical Sciences, University of Catania, Catania, Italy. f.damico@unict.it
Skarmoutsou
Evangelia
E
Mangano
Katia
K
Malaponte
Grazia
G
Stivala
Franca
F
Mazzarino
Maria Clorinda
MC
D000071916:AC133 Antigen; D000818:Animals; D015703:Antigens, CD / Q000032:analysis* / Q000235:genetics*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D006023:Glycoproteins / Q000032:analysis* / Q000235:genetics*; D007545:Isoproterenol / Q000008:administration & dosage / Q000494:pharmacology*; D010306:Parotid Gland / Q000187:drug effects* / Q000648:ultrastructure; D010455:Peptides / Q000032:analysis* / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar
D000071916:AC133 Antigen; D015703:Antigens, CD; D006023:Glycoproteins; D010455:Peptides; C000606025:Prom1 protein, rat; D012333:RNA, Messenger; D007545:Isoproterenol