The effects of rapamycin on lens epithelial cell proliferation, migration, and matrix formation: an in vitro study.
OBJECTIVE
The objective of the present study was to investigate the efficacy of rapamycin on rabbit lens epithelial cell proliferation, migration, and secretion of extracellular matrix fibronectin (Fn).


METHODS
Rabbit lens epithelium cells (rLECs) were isolated from 1 month old rabbit. rLECs were either cultured for 24, 48, or 72 h with different doses of rapamycin (0.1, 1, and 10 ng/ml). The proliferation kinetics, proliferating cell nuclear antigen (PCNA) expression, and basic fibroblast growth factor (bFGF)-induced migration of rLEC was determined by methyl thiazol tetrazolium (MTT) assay, western blotting and transwell chamber assay, respectively. The effect of rapamycin on the synthesis of Fn was examined via immunofluorescence.


RESULTS
Rapamycin significantly inhibited rLEC proliferation and PCNA protein expression when administered doses and time periods except for 0.1 ng/ml for 24 h. bFGF-induced migration rLECs was inhibited by pretreatment with rapamycin for 48 h. Extracellular matrix Fn formation of rLECs was also reduced by rapamycin.


CONCLUSIONS
In our study, rapamycin strongly inhibited rLEC proliferation, bFGF-induced migration, and extracellular matrix Fn formation. Thus, rapamycin may have a potential inhibition of posterior capsule opacification (PCO) and needs further study.
Liu
Hongling
H
Department of Ophthalmology, The 1st Affiliated Hospital, Harbin Medical University, Harbin, PR China.
Feng
Guangzhong
G
Wu
Lan
L
Fu
Shaoying
S
Liu
Ping
P
Yang
Wei
W
Zhang
Xiaomei
X
D000818:Animals; D015153:Blotting, Western; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D004847:Epithelial Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D005109:Extracellular Matrix / Q000187:drug effects* / Q000378:metabolism*; D005353:Fibronectins / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D007908:Lens, Crystalline / Q000166:cytology*; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D011817:Rabbits; D020123:Sirolimus / Q000494:pharmacology*
D005353:Fibronectins; D018809:Proliferating Cell Nuclear Antigen; D020123:Sirolimus
J Clin Invest. 1996 Nov 15;98(10):2277-83
8941644
Ophthalmologe. 1994 Feb;91(1):98-102
8173260
J Immunol Methods. 1983 Dec 16;65(1-2):55-63
6606682
Exp Mol Med. 2006 Apr 30;38(2):173-9
16672771
Tissue Cell. 1986;18(1):83-97
3515629
Circulation. 2001 Sep 4;104(10):1188-93
11535578
J Cell Biol. 1988 Apr;106(4):1365-73
3360855
Graefes Arch Clin Exp Ophthalmol. 2009 Jun;247(6):801-7
19066932
N Engl J Med. 2003 Oct 2;349(14):1315-23
14523139
Graefes Arch Clin Exp Ophthalmol. 2003 Apr;241(4):309-13
12719992
Exp Eye Res. 1992 Mar;54(3):393-401
1521568
Int J Dev Biol. 2004;48(8-9):857-65
15558477
J Cell Physiol. 1982 Nov;113(2):273-80
7174730
Br J Ophthalmol. 2000 Mar;84(3):332-6
10684849
J Fr Ophtalmol. 1997;20(9):664-70
9587577
J Cell Biol. 1986 Jan;102(1):179-88
3941152
Invest Ophthalmol Vis Sci. 1995 Oct;36(11):2304-12
7558725
Mol Vis. 2006 Oct 26;12:1233-42
17110906
Circulation. 2003 Feb 11;107(5):777-84
12578884
J Cell Physiol. 1985 Dec;125(3):393-402
3905828
Differentiation. 1984;28(1):53-61
6394411
Surv Ophthalmol. 1992 Sep-Oct;37(2):73-116
1455302
J Cataract Refract Surg. 1996;22 Suppl 1:835-40
9279681
Ophthalmology. 1983 Dec;90(12):1548-53
6677855