Canine reference intervals for coagulation markers using the STA Satellite(R) and the STA-R Evolution(R) analyzers.
The aim of the current study was to determine canine reference intervals for prothrombin time (PT), activated partial thromboplastin time (APTT), fibrinogen, and antithrombin (AT) according to international recommendations. The STA Satellite(R) coefficients of variation of within-laboratory imprecision were 3.9%, 1.3%, 6.9%, and 5.1% for PT, APTT, fibrinogen, and AT, respectively. At 4 degrees C, citrated specimens were stable up to 8 hr for whole blood and 36 hr for plasma, except for APTT, which increased slightly (<1 sec). Nonparametric reference intervals determined in citrated plasma from 139 healthy fasting purebred dogs were 6.9-8.8 sec, 13.1-17.2 sec, 1.24-4.30 g/l, and 104-188% for PT, APTT, fibrinogen, and AT, respectively. Based on Passing-Bablok comparison between STA Satellite and STA-R Evolution(R) using 60 frozen specimens from a canine plasma bank, the corresponding reference intervals were transferred to the STA-R Evolution: 7.1-9.2 sec, 12.9-17.3 sec, 1.20-4.43 g/l, and 94-159% for PT, APTT, fibrinogen, and AT, respectively.
Geffré
Anne
A
Département des Sciences Cliniques, Ecole Nationale Vétérinaire de Toulouse, Toulouse, France. a.geffre@envt.fr
Grollier
Sophie
S
Hanot
Camille
C
Vergez
François
F
Trumel
Catherine
C
Braun
Jean-Pierre
JP
D000375:Aging / Q000502:physiology; D000818:Animals; D000991:Antithrombins / Q000032:analysis*; D001777:Blood Coagulation; D019411:Clinical Laboratory Techniques; D004285:Dogs / Q000254:growth & development / Q000502:physiology; D005260:Female; D005340:Fibrinogen / Q000032:analysis*; D007753:Laboratories; D008297:Male; D010052:Ovariectomy; D010314:Partial Thromboplastin Time / Q000662:veterinary; D012016:Reference Values; D017215:Satellite Communications; D013045:Species Specificity
D000991:Antithrombins; D005340:Fibrinogen