Molecular cloning of IKKβ from the mandarin fish Siniperca chuatsi and its up-regulation in cells by ISKNV infection.
The IκB kinase β (IKKβ) plays crucial roles in regulating activation of nuclear factor-kappa B (NF-κB) in response to proinflammatory factors and microbial and viral infections. Here, we report the cloning of an IKKβ cDNA (named SicIKKβ) from the mandarin fish Siniperca chuatsi. The full-length cDNA is 4052bp and contains an ORF that encodes a predicted protein of 743-amino acid residues. The deduced amino acid sequence of SicIKKβ has the same domain organization as human IKKβ, which consists of a serine/threonine kinase domain, a leucine zipper motif and a putative helix-loop-helix motif. Quantitative RT-PCR showed that SicIKKβ was ubiquitously expressed in tissues of mandarin fish, and its expression in mandarin fish fry (MFF-1) cells was up-regulated during the course of ISKNV infection.
Chen
Wei-Jian
WJ
State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, PR China.
Guo
Chang-Jun
CJ
Zhou
Zhi-Cheng
ZC
Yuan
Li-Qun
LQ
Xiang
Zhi-Ming
ZM
Weng
Shao-Ping
SP
Zhang
Ying-Fen
YF
Yu
Xiao-Qing
XQ
He
Jian-Guo
JG
D000818:Animals; D003001:Cloning, Molecular; D004266:DNA Virus Infections / Q000201:enzymology / Q000235:genetics / Q000276:immunology / Q000662:veterinary*; D005393:Fish Diseases / Q000201:enzymology* / Q000235:genetics / Q000276:immunology; D051550:I-kappa B Kinase / Q000235:genetics*; D007497:Iridoviridae / Q000276:immunology; D016328:NF-kappa B / Q000378:metabolism; D010473:Perciformes / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D017422:Sequence Analysis, DNA / Q000662:veterinary; D015854:Up-Regulation
D016328:NF-kappa B; D051550:I-kappa B Kinase