title: In vivo discrimination of type 3 secretion system-positive and -negative Pseudomonas aeruginosa via a caspase-1-dependent pathway.
abstract: Microbe-associated molecular patterns are recognized by Toll-like receptors of the innate immune system. This recognition enables a rapid response to potential pathogens but does not clearly provide a way for the innate immune system to discriminate between virulent and avirulent microbes. We find that pulmonary infection of mice with type 3 translocation-competent Pseudomonas aeruginosa triggers a rapid inflammatory response, while infection with isogenic translocation-deficient mutants does not. Discrimination between translocon-positive and -negative bacteria requires caspase-1 activity in bone marrow-derived cells and interleukin-1 receptor signaling. Thus, the activation of caspase-1 by bacteria expressing type 3 secretion systems allows for rapid recognition of bacteria expressing conserved functions associated with virulence.
authors.affiliation: Department of Internal Medicine (Infectious Diseases), Yale University School of Medicine, New Haven, CT 06520, USA.
authors.forename: Tamding
authors.initials: T
authors.lastname: Wangdi
authors.forename: Lilia A
authors.initials: LA
authors.lastname: Mijares
authors.forename: Barbara I
authors.initials: BI
authors.lastname: Kazmierczak
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000276:immunology / Q000378:metabolism*; D001854:Bone Marrow Cells / Q000276:immunology; D020170:Caspase 1 / Q000378:metabolism*; D004847:Epithelial Cells / Q000382:microbiology; D007113:Immunity, Innate; D008168:Lung / Q000276:immunology / Q000382:microbiology; D016676:Macrophages, Alveolar / Q000276:immunology / Q000382:microbiology; D051379:Mice; D008810:Mice, Inbred C57BL; D020556:Neutrophil Infiltration; D011552:Pseudomonas Infections / Q000201:enzymology / Q000276:immunology* / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000276:immunology / Q000378:metabolism / Q000472:pathogenicity*; D017472:Receptors, Interleukin-1 / Q000378:metabolism; D015398:Signal Transduction; D014132:Trachea / Q000166:cytology / Q000382:microbiology; D014774:Virulence
chemical_list: D001426:Bacterial Proteins; D017472:Receptors, Interleukin-1; D020170:Caspase 1
references.citation: J Infect Dis. 2001 Jun 15;183(12):1767-74
references.pmid: 11372029
references.citation: Infect Immun. 2003 Apr;71(4):1630-4
references.pmid: 12654774
references.citation: Cell Microbiol. 2005 Nov;7(11):1635-46
references.pmid: 16207250
references.citation: Am J Physiol Lung Cell Mol Physiol. 2002 Feb;282(2):L285-90
references.pmid: 11792633
references.citation: EMBO J. 2003 Oct 1;22(19):4957-67
references.pmid: 14517235
references.citation: Infect Immun. 2005 Mar;73(3):1695-705
references.pmid: 15731070
references.citation: PLoS Pathog. 2008 Nov;4(11):e1000220
references.pmid: 19043549
references.citation: J Biol Chem. 2010 Apr 2;285(14):10508-18
references.pmid: 20097760
references.citation: Eur J Immunol. 2007 Nov;37(11):3030-9
references.pmid: 17935074
references.citation: Am J Physiol Lung Cell Mol Physiol. 2002 Dec;283(6):L1315-21
references.pmid: 12388377
references.citation: Microbiology (Reading). 2001 Oct;147(Pt 10):2659-2669
references.pmid: 11577145
references.citation: Annu Rev Immunol. 2002;20:197-216
references.pmid: 11861602
references.citation: Biochemistry. 2006 Jul 4;45(26):8117-23
references.pmid: 16800636
references.citation: PLoS Pathog. 2007 Aug 10;3(8):e111
references.pmid: 17696608
references.citation: Nature. 2001 Apr 26;410(6832):1099-103
references.pmid: 11323673
references.citation: J Infect Dis. 1966 Oct;116(4):481-9
references.pmid: 4959184
references.citation: PLoS Pathog. 2009 Dec;5(12):e1000686
references.pmid: 19997504
references.citation: J Exp Med. 2007 Dec 24;204(13):3235-45
references.pmid: 18070936
references.citation: J Immunol. 1996 Dec 15;157(12):5221-4
references.pmid: 8955165
references.citation: Proc Natl Acad Sci U S A. 2008 Feb 19;105(7):2562-7
references.pmid: 18256184
references.citation: Infect Immun. 2004 Oct;72(10):6176-80
references.pmid: 15385528
references.citation: Microbes Infect. 2000 Jul;2(9):1051-60
references.pmid: 10967285
references.citation: Cell. 2008 Mar 7;132(5):818-31
references.pmid: 18329368
references.citation: Crit Care Med. 2002 Mar;30(3):521-8
references.pmid: 11990909
references.citation: Cell Host Microbe. 2010 May 20;7(5):376-87
references.pmid: 20478539
references.citation: Proc Natl Acad Sci U S A. 2010 Feb 16;107(7):3076-80
references.pmid: 20133635
references.citation: J Clin Microbiol. 2004 Nov;42(11):5229-37
references.pmid: 15528719
references.citation: Infect Immun. 2006 Aug;74(8):4462-73
references.pmid: 16861632
references.citation: Infect Immun. 1984 Aug;45(2):470-4
references.pmid: 6086529
references.citation: Science. 1995 Mar 31;267(5206):2000-3
references.pmid: 7535475
references.citation: Infect Immun. 1996 Dec;64(12):4984-92
references.pmid: 8945536
references.citation: PLoS One. 2009 Oct 06;4(10):e7259
references.pmid: 19806220
references.citation: Cell Host Microbe. 2009 Aug 20;6(2):125-36
references.pmid: 19683679
references.citation: Cell Host Microbe. 2009 Jul 23;6(1):10-21
references.pmid: 19616762
references.citation: Infect Immun. 2004 Aug;72(8):4741-50
references.pmid: 15271936
references.citation: Nat Immunol. 2006 Jun;7(6):569-75
references.pmid: 16648853
references.citation: Infect Immun. 1998 Apr;66(4):1413-20
references.pmid: 9529061
references.citation: Infect Immun. 2000 Dec;68(12):7100-13
references.pmid: 11083836
references.citation: Infect Immun. 2009 May;77(5):1981-91
references.pmid: 19237518
references.citation: Infect Immun. 2009 Dec;77(12):5300-10
references.pmid: 19805527
references.citation: J Med Microbiol. 2010 May;59(Pt 5):511-520
references.pmid: 20093376
references.citation: Cell. 2007 Sep 7;130(5):918-31
references.pmid: 17803913
references.citation: Ann Clin Microbiol Antimicrob. 2005 Dec 23;4:22
references.pmid: 16375761
references.citation: Semin Immunol. 2009 Aug;21(4):199-207
references.pmid: 19539499
references.citation: Annu Rev Immunol. 2009;27:519-50
references.pmid: 19302047
references.citation: J Cell Mol Med. 2008 Sep-Oct;12(5A):1767-76
references.pmid: 18081695