[The protective effect of keratinocyte growth factor on lipopolysaccharide-induced acute lung injury in rat].
OBJECTIVE
To investigate the protective effect of keratinocyte growth factor (KGF) on lipopolysaccharide (LPS)-induced acute lung injury (ALI) and its potential mechanism in rats.


METHODS
Thirty-six Sprague-Dawley (SD) rats were randomly divided into three groups, each group with 12 rats. LPS (5 mg/kg) was injected intravenously to induce ALI in model group, and same amount of normal saline was injected in control group and KGF group. The rats in KGF group were treated with KGF (5 mg/kg) intratracheally after injection of LPS. The rats were sacrificed after 8 hours, histologic assessments, wet/dry weight (W/D) ratio, pulmonary vascular permeability, lung epithelial cell permeability, the proliferation and repair capacity of type II alveolar epithelial cells (ATII) were analyzed.


RESULTS
Under optical microscope, it was found that KGF could reduce injury to lung tissue induced by LPS. Compared with the model group, KGF could decrease pulmonary vascular permeability [(0.026+/-0.049)% vs. (0.087+/-0.027)%], lung epithelial cell permeability [(0.692+/-0.017)% vs. (0.931+/-0.029)%] and W/D ratio (4.778+/-0.243 vs. 6.869+/-0.153, P<0.05 or P<0.01), enhance the proliferation and repair capacity of ATII cells (ATII cells: 6.083+/-1.781 vs. 4.666+/-1.923, injury area (mm2): 2.946+/-0.453 vs. 6.181+/-0.975, P<0.05 and P<0.01).


CONCLUSIONS
KGF could reduce the injury to the lung in LPS-induced ALI, and it plays a protective role through enhancing the proliferation and repair capacity of ATII cells.
Hu
Xiao-wei
XW
Department of Pediatric Intensive Care Unit, Shanghai Children's Medical Center, Shanghai Jiaotong University School of Medicine, Shanghai 200127, China.
Zhang
Xiao-ping
XP
Li
Bi-ru
BR
Zhang
Jian
J
Wang
Ying
Y
D055371:Acute Lung Injury / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D000818:Animals; D004195:Disease Models, Animal; D051523:Fibroblast Growth Factor 7 / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000633:toxicity*; D008168:Lung / Q000187:drug effects / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley
D008070:Lipopolysaccharides; D051523:Fibroblast Growth Factor 7