title: [Improvement of solubility of puerarin through deoxycholate/phospholipid mixed micelle preparing technology].
abstract: OBJECTIVE
  To prepare puerarin deoxycholate/phospholipid mixed micelle to increase the solubility of puerarin.
  
  
  METHODS
  Sodium deoxycholate and soybean phospholipids were used to prepare puerarin mixed micelle through orthogonal design experiments. With the solubility, shape and particle size as the response indexes, the preparing process of puerarin mixed micelle was optimized.
  
  
  RESULTS
  The optimized process for the puerarin deoxycholate/phospholipid mixed micelle was that the puerarin, soya phosphatidylcholine and sodium deoxycholate with the mole ratio of 3:2:4 should be dissolved in methanol-chloroform (1:1), and the solvents should be evaporated rotatively at 30 degrees C. The particle diameter of the mixed micelle was (64.8 +/- 13) nm (volume-weighted particle size distribution), and the solubility was 0.811 1 g x L(-1) in water at the room temperature, which was 22.3 times as that of the raw puerarin (0.036 4 g x L-1).
  
  
  CONCLUSIONS
  The puerarin deoxycholate/phospholipid mixed micelle can improve the solubility of puerarin significantly.
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Bejing 100102, China. hedianhong_2006@yahoo.com.cn
authors.forename: Dianhong
authors.initials: D
authors.lastname: He
authors.forename: Liwei
authors.initials: L
authors.lastname: Han
authors.forename: Yuan
authors.initials: Y
authors.lastname: Wang
authors.forename: Jian
authors.initials: J
authors.lastname: Ni
authors.forename: Shouying
authors.initials: S
authors.lastname: Du
mesh_terms: D003840:Deoxycholic Acid / Q000737:chemistry*; D007529:Isoflavones / Q000737:chemistry*; D008823:Micelles; D010316:Particle Size; D010743:Phospholipids / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D012995:Solubility
chemical_list: D007529:Isoflavones; D008823:Micelles; D010743:Phospholipids; D010936:Plant Extracts; D003840:Deoxycholic Acid; C033607:puerarin