[Establishment of transformation system in mulberry and biosynthesis of quercetin].
OBJECTIVE
To establish the transformation system of mulberry, and test its ability of quercetin biosynthesis.


METHODS
Hairy roots of mulberry were obtained through infecting etiolated seedlings with Agrobacterium tumefaciens strain C58C1. The culture condition of hairy roots was optimized. The transformation of T-DNA was examined by PCR assay and quercetin content was determined by HPLC.


RESULTS
When infecting stem cutting of etiolated seedlings via C58C1 strain, the optimal transformation conditions were as follows: 10 minutes' infection, two-days pre-culture and co-culture, additional hydroxylacetosyringone (As) 100 mg x L(-1). The PCR examination result showed that rolB and rolC genes could be inserted into the hairy roots of mulberry. Hairy roots appeared in 10 days after infecting, the frequency of stems explants was up to 92% after 30 days culturing. After 50 days culturing in 1/2MS + 0.05 mg x L(-1) IBA liquid medium, the content of quercetin increased by 8. 5-fold.


CONCLUSIONS
Hairy root culture system of Moraceae plants was established successfully for the first time. In addition, it also provides a foundation for further industrial production of active compounds such as quercetin.
Li
Xiangyun
X
School of Life Science, Southwest University, Chongqing 400715, China. yun731@swu.edu.cn
Zhu
Hong
H
Sun
Yiming
Y
Sun
Min
M
D016960:Agrobacterium tumefaciens / Q000235:genetics / Q000378:metabolism; D002478:Cells, Cultured; D004269:DNA, Bacterial / Q000235:genetics; D018390:Gene Targeting / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D031625:Morus / Q000235:genetics* / Q000378:metabolism* / Q000382:microbiology; D011794:Quercetin / Q000096:biosynthesis*; D014170:Transformation, Genetic
D004269:DNA, Bacterial; C036483:T-DNA; D011794:Quercetin