Prevalence of primary biliary cirrhosis in adults referring hospital for annual health check-up in Southern China.
BACKGROUND
Primary biliary cirrhosis (PBC) is an autoimmune liver disease characterized by the presence of anti-mitocondrial autoantibodies (AMA) which has an essential role also for diagnosis. In addition, also some anti-nuclear antibodies (ANA) have been shown to be highly specific PBC. The purpose of this study was to assess the prevalence of PBC among the adults referring hospital for annual health check-up in Southern China by screening sera for PBC-specific autoantibodies.


METHODS
AMA and ANA were screened in 8,126 adults (mean age 44 ± 15 years, 48% females) by indirect immunofluorenscence (IIF). Positive sera were tested by ELISA/immunoblotting for AMA-M2, anti-sp100 and anti-gp210. A diagnosis of PBC was re-assessed six months after the initial testing.


RESULTS
Out of 8,126 individuals 35 were positive for AMA and 79 positive for ANA. Nineteen, 4, and 3 of the subjects positive for AMA and/or ANA showed reactivity for AMA-M2, anti-sp100 or gp210, respectively, further tested with ELISA/immunoblotting. Fourteen in the 39 individuals positive for AMA at IIF, AMA-M2, anti-gp210, or anti-sp100 had abnormal cholestatic liver functional indices. One definite and 3 probable PBC diagnosis could be made in 4 cases including 3 females and 1 male after half a year.


CONCLUSIONS
We found a point prevalence rate of PBC among Southern Chinese adults attending for yearly health check-up of 492 cases per million (95% CI, 128 to 1,093) and 1,558 cases per million (95% CI, 294 to 3,815) for women over 40, a finding similar to prevalence reported in other geographical areas.
Liu
Haiying
H
Clinical Laboratory, Guangzhou Women and Children’s Medical Center, 9 Jinsui Road, Guangzhou 510623, China.
Liu
Yunfeng
Y
Wang
Luxia
L
Xu
Dexing
D
Lin
Bingliang
B
Zhong
Renqian
R
Gong
Sitang
S
Podda
Mauro
M
Invernizzi
Pietro
P
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000974:Antibodies, Antinuclear / Q000032:analysis*; D001323:Autoantibodies / Q000032:analysis*; D002681:China / Q000453:epidemiology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005500:Follow-Up Studies; D006304:Health Status; D006801:Humans; D015151:Immunoblotting; D008105:Liver Cirrhosis, Biliary / Q000175:diagnosis / Q000453:epidemiology* / Q000276:immunology; D008297:Male; D008403:Mass Screening / Q000379:methods*; D008875:Middle Aged; D008928:Mitochondria / Q000276:immunology; D015995:Prevalence; D055815:Young Adult
D000974:Antibodies, Antinuclear; D001323:Autoantibodies
J Immunol. 1987 Dec 1;139(11):3828-33
2445820
Liver Int. 2008 Nov;28(9):1305-13
18346129
Clin Liver Dis. 2008 May;12(2):289-303; viii
18456181
Am J Gastroenterol. 2002 Sep;97(9):2402-7
12358264
J Clin Gastroenterol. 2009 Aug;43(7):676-9
19247207
Hepatology. 2009 Dec;50(6):1884-92
19821525
J Gastroenterol. 2001 Apr;36(4):248-54
11324728
Semin Liver Dis. 2007 May;27(2):161-72
17520516
Hepatology. 1996 Jul;24(1):97-103
8707289
Am J Gastroenterol. 1997 Jan;92(1):124-6
8995951
J Gastroenterol Hepatol. 2008 Apr;23(4):599-605
17645483
World J Gastroenterol. 2008 Jun 7;14(21):3374-87
18528935
Clin Exp Med. 2008 Mar;8(1):1-7
18385934
J Autoimmun. 2010 May;34(3):J300-6
20036105
World J Gastroenterol. 2003 Sep;9(9):2128-31
12970922
J Autoimmun. 2009 Aug;33(1):12-6
19356902
J Hepatol. 2004 Oct;41(4):677-83
15464251
J Gastroenterol Hepatol. 2008 Apr;23(4):508-11
18397481
Scand J Gastroenterol. 2007 Nov;42(11):1347-53
17918011
World J Gastroenterol. 2005 Jul 14;11(26):4108-10
15996040
Gastroenterology. 2004 Aug;127(2):470-5
15300579
J Gastroenterol Hepatol. 2008 Apr;23(4):592-8
17645470
Hepatology. 2007 Mar;45(3):659-65
17326160
N Engl J Med. 2005 Sep 22;353(12):1261-73
16177252
Semin Liver Dis. 2005 Aug;25(3):298-310
16143945
Hepatology. 1999 Aug;30(2):390-4
10421645
Isr Med Assoc J. 2005 Nov;7(11):717-21
16308995
J Hepatol. 2009 Feb;50(2):227-42
19054588
Zhonghua Gan Zang Bing Za Zhi. 2002 Oct;10(5):325
12392604
Autoimmun Rev. 2008 Sep;7(8):626-30
18603021
Liver. 1996 Dec;16(6):377-9
9021716
Hepatol Res. 2003 Aug;26(4):330-336
12963434
Gastroenterology. 2000 Dec;119(6):1631-6
11113084
Virchows Arch A Pathol Anat Histol. 1978 Aug 22;379(2):103-12
150690
Eur J Gastroenterol Hepatol. 2007 Oct;19(10):859-64
17873609