Characterization and preparation of core-shell type nanoparticle for encapsulation of anticancer drug.
The aim of this study is to prepare delivery vehicles of paclitaxel using low molecular weight water-soluble chitosan (LMWSC) and evaluate them as an anticancer drug delivery system. LMWSC was modified with methoxy polyethylene glycol (LMWSC-MPEG, ChitoPEG), and then it was conjugated with cholesterol (LMWSC-MPEG-Chol). Core-shell type LMWSC-MPEG-Chol nanoparticles (LMWSC-NPs) were prepared by the dialysis method, and the core-shell structure was confirmed by 1H NMR analysis. To this polymer, paclitaxel was encapsulated and core-shell type nanoparticles were prepared. The release tests indicated that release of paclitaxel from the core-shell type nanoparticles and its transport across the dialysis membrane was slower than dialysis of free paclitaxel. In a cytotoxicity study using CT26 cell, the paclitaxel-encapsulated core-shell type nanoparticles (LMWSC-NPs) showed a toxicity against tumor cells similar to paclitaxel itself. The results of a tumor inhibition test with CT26 implanted upon mouse tumor models in vivo indicated that the application of a dose of 10 mg/kg of LMWSC-NPT showed a superior survival rate, and a slower tumor growth than when paclitaxel alone was administered, although the tumor growth and survival rate were not significantly changed at a dose of 2 mg/kg. The LMWSC-NPT dose above 10mg/kg showed a superior antitumor activity.
Jang
Mi-Kyeong
MK
Department of Polymer Science & Engineering, Sunchon National University, 315 Maegok, Suncheon, Jeonnam 540-742, Republic of Korea.
Jeong
Young-Il
YI
Nah
Jae-Woon
JW
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry; D002784:Cholesterol / Q000737:chemistry; D004195:Disease Models, Animal; D004337:Drug Carriers / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D016503:Drug Delivery Systems; D015195:Drug Design; D004354:Drug Screening Assays, Antitumor; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D008968:Molecular Conformation; D008970:Molecular Weight; D053758:Nanoparticles / Q000737:chemistry*; D009368:Neoplasm Transplantation; D017239:Paclitaxel / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D010316:Particle Size; D011092:Polyethylene Glycols / Q000737:chemistry; D012995:Solubility; D013499:Surface Properties
D000972:Antineoplastic Agents, Phytogenic; D004337:Drug Carriers; D011092:Polyethylene Glycols; C028210:monomethoxypolyethylene glycol; D048271:Chitosan; D002784:Cholesterol; D017239:Paclitaxel