Antioxidant profile of whole saliva after scaling and root planing in periodontal disease.
OBJECTIVE
This study compared the total antioxidant status (TAS) and superoxide dismutase (SOD) activity in the saliva of periodontally compromised patients before and after scaling and root planing (SRP) to assess their diagnostic utility.


METHODS
Severe chronic periodontitis patient (test group) and subjects with no attachment loss, sites showing a 3 mm or more probing depth and a sulcus bleeding index < 10% (control group) were enrolled in this study. Saliva sampling and clinical examination were performed at one week, one month and 3 months after SRP. The TAS and SOD activity in each patient's saliva was measured for the comparative analysis between the groups.


RESULTS
In the test group, the TAS decreased directly after SRP. With time, it increased slightly and was relatively unchanged compared to the baseline. In the control group, the TAS also decreased immediately after SRP but increased gradually with time until 3 months. The SOD activity in the test and control subjects decreased immediately after SRP until 1 month. At 3 months, the SOD activity had increased. Both groups had a similar profile of SOD activity. However, the SOD activity of the control group was significantly higher than that of the test group at each point in time (P < 0.05).


CONCLUSIONS
There was a significant difference in the total salivary antioxidant level between the periodontitis and healthy or gingivitis (control) group during the experiment period. The total antioxidant level in the saliva was higher in the patients with severe chronic periodontitis than the healthy or gingivitis control before SRP. The SOD activity of the periodontitis patients was lower than the control at each time point. These findings conclusively reveal the possible use of saliva as a diagnostic tool for periodontal health.
Kim
Sang-Chul
SC
Department of Periodontology, Chonnam National University School of Dentistry, Gwangju, Korea.
Kim
Ok-Su
OS
Kim
Ok-Joon
OJ
Kim
Young-Joon
YJ
Chung
Hyun-Ju
HJ
Ann Clin Biochem. 1997 Jul;34 ( Pt 4):412-21
9247675
Lab Invest. 1985 Dec;53(6):656-63
2999509
Clin Oral Implants Res. 2007 Feb;18(1):27-33
17224020
J Clin Periodontol. 2004 Jul;31(7):515-21
15191586
Crit Rev Oral Biol Med. 1999;10(4):458-76
10634583
Clin Sci (Lond). 2003 Aug;105(2):167-72
12650638
Clin Diagn Lab Immunol. 2003 Sep;10(5):897-902
12965924
Clin Oral Investig. 2000 Mar;4(1):21-4
11218511
J Periodontol. 2007 Aug;78(8):1602-11
17668980
Free Radic Res. 1994 Nov-Dec;21(6):417-25
7834056
J Periodontol. 2006 Sep;77(9):1533-8
16945031
J Clin Periodontol. 1997 May;24(5):287-96
9178107
J Periodontal Res. 1984 Jul;19(4):390-400
6205132
Mol Pathol. 2002 Dec;55(6):367-73
12456773
Dig Dis Sci. 1997 Oct;42(10):2175-81
9365155
J Oral Pathol Med. 1998 Mar;27(3):101-5
9563800
J Clin Pediatr Dent. 1998 Summer;22(4):341-5
9796506
Am J Med. 1991 Sep 30;91(3C):14S-22S
1928205
J Periodontal Res. 2005 Oct;40(5):378-84
16105090
J Clin Periodontol. 2005 Mar;32(3):238-43
15766365