[Association of CTLA4 gene +49G/A polymorphism with HBV infection and HBV-related hepatocellular carcinoma in Hunan Han population].
OBJECTIVE
To investigate the relationship between CTLA4 +49G/A SNP polymorphism and the susceptibility to hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC).


METHODS
CTLA4+49G/A single nucleotide polymorphism (SNP) was analyzed by DNA sequencing in 165 control subjects, 155 patients with chronic hepatitis B (CHB) and 149 HCC patients. Serum HBsAg, HBeAg and AFP levels were measured in all the subjects.


RESULTS
In HCC and CHB groups, the genotype frequency was 40.3% and 50.0% for GG , and 59.7% and 50.0% for AG+AA, respectively, while the genotype frequency was 61.8% for GG and 38.2% for AG+AA in the control group. In HCC group, CHB group and controls, the A allele frequencies was 44.6%, 37.4% and 28.8%, and the G allele frequencies was 55.4%, 62.6% and 71.2%, respectively. Significant differences were found not only in the allele frequencies (P<0.05) but also in AA and combined (AA+AG) genotype frequencies (P<0.05) between the 3 groups.


CONCLUSIONS
+49G/A SNP of the CTLA4 gene can be associated with HBV and HBV-related HCC.
Liu
Gang
G
Institute of Reproduction and Stem Cell Engineering, Central South University, Changsha 410078, China. lg5809cn@yahoo.com.cn
Lu
Guang-xiu
GX
D000328:Adult; D044466:Asian People / Q000235:genetics; D060908:CTLA-4 Antigen / Q000235:genetics*; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000821:virology; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006509:Hepatitis B / Q000235:genetics*; D006514:Hepatitis B Surface Antigens / Q000097:blood; D006513:Hepatitis B e Antigens / Q000097:blood; D006515:Hepatitis B virus; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000821:virology; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide
D060908:CTLA-4 Antigen; C556706:CTLA4 protein, human; D006514:Hepatitis B Surface Antigens; D006513:Hepatitis B e Antigens