Patterns of survival and volatile metabolites of selected Lactobacillus strains during long-term incubation in milk.
The focus of this study was to monitor the survival of populations and the volatile compound profiles of selected Lactobacillus strains during long-term incubation in milk. The enumeration of cells was determined by both the Direct Epifluorescent Filter Technique using carboxyfluorescein diacetate (CFDA) staining and the plate method. Volatile compounds were analysed by the gas-chromatography technique. All strains exhibited good survival in cultured milks, but Lactobacillus crispatus L800 was the only strain with comparable growth and viability in milk, assessed by plate and epifluorescence methods. The significant differences in cell numbers between plate and microscopic counts were obtained for L. acidophilus strains. The investigated strains exhibited different metabolic profiles. Depending on the strain used, 3 to 8 compounds were produced. The strains produced significantly higher concentrations of acetic acid, compared to other volatiles. Lactobacillus strains differed from one another in number and contents of the volatile compounds.
Łaniewska-Trokenheim
Łucja
Ł
Industrial and Food Microbiology, Faculty of Food Science, University of Warmia and Mazury, Pl. Cieszyński 1, Olsztyn, Poland.
Olszewska
Magdalena
M
Miks-Krajnik
Marta
M
Zadernowska
Anna
A
D000818:Animals; D002417:Cattle; D005516:Food Microbiology; D007778:Lactobacillus / Q000737:chemistry / Q000502:physiology*; D008892:Milk / Q000737:chemistry / Q000382:microbiology*; D055549:Volatile Organic Compounds / Q000032:analysis / Q000378:metabolism*
D055549:Volatile Organic Compounds
Cryobiology. 1998 Jun;36(4):315-9
9679081
Appl Environ Microbiol. 2001 May;67(5):2326-35
11319119
Appl Environ Microbiol. 1999 Aug;65(8):3681-9
10427066
J Gen Appl Microbiol. 2003 Oct;49(5):301-7
14673754
Int J Food Microbiol. 2006 Oct 1;111(3):197-205
16934355
J Dairy Sci. 1995 Nov;78(11):2326-35
8747323
Appl Environ Microbiol. 2001 Jan;67(1):420-5
11133474
Int J Food Microbiol. 2003 Oct 15;87(1-2):17-27
12927703
J Dairy Sci. 1995 Aug;78(8):1657-64
8786251
Food Microbiol. 2006 Sep;23(6):571-7
16943053
J Appl Microbiol. 1998 Apr;84(4):593-9
9633657
Microbes Infect. 2000 Oct;2(12):1523-35
11099939
Int J Food Microbiol. 2004 Jan 15;90(2):139-59
14698096
Appl Environ Microbiol. 2002 Jun;68(6):2934-42
12039752
Cryobiology. 2007 Aug;55(1):35-43
17577587