A neuroprotective polysaccharide from Hyriopsis cumingii.
A water-soluble polysaccharide from Hyriopsis cumingii (HCp) was isolated by hot-water extraction and ethanol precipitation. We investigated the preliminary neuroprotective activity of HCp on cerebral ischemia/reperfusion (I/R)-induced rat brain tissue injury using the middle cerebral artery occlusion (MCAO) model. The structural analysis showed that the major component of HCp (HP-I) is an α-(1→4)-d-glucan branched with a single α-d-glucose at C-6 every five residues on average and has a weight-average molecular weight of about 2.65 × 10(5). Pharmacological studies revealed that HCp improves and restores nerve function impairment, significantly increases brain tissue total superoxide dismutase activity, lowers malondialdehyde content effectively, and reduces brain levels of caspase-3 mRNA expression in a dose-effect manner, indicating that H. cumingii polysaccharide possesses significant neuroprotective effects.
Hu
Changfeng
C
Department of Chemistry, Zhejiang University, Hangzhou 310027, People's Republic of China.
Li
Jinhui
J
Yang
Deyu
D
Pan
Yuanjiang
Y
Wan
Haitong
H
D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000503:physiopathology; D001930:Brain Injuries; D002545:Brain Ischemia / Q000188:drug therapy*; D053148:Caspase 3 / Q000032:analysis / Q000235:genetics; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D008315:Malondialdehyde / Q000032:analysis; D018696:Neuroprotective Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D012995:Solubility; D013482:Superoxide Dismutase / Q000032:analysis; D049876:Unionidae / Q000737:chemistry*; D014867:Water
C552906:HP-I polysaccharide; D018696:Neuroprotective Agents; D011134:Polysaccharides; D012333:RNA, Messenger; D014867:Water; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D053148:Caspase 3