title: Protein analysis of laser capture micro-dissected tissues revealed cell-type specific biological functions in developing barley grains.
abstract: Both the nucellar projection (NP) and endosperm transfer cells (ETC) of the developing barley grain (harvested 8 days after flowering) were isolated by laser capture micro-dissection combined with pressure catapulting. Protein extracts were analyzed by nanoUPLC separation combined with ESI-Q-TOF mass spectrometry. The majority of the ~160 proteins identified were involved in translation, protein synthesis, or protein destination. The NP proteome was enriched for stress defense molecules, while proteins involved in assimilate transport and the mobilization of nutrients were common to both the NP and the ETC. The combined qualitative and quantitative protein profiling allowed for the identification of several proteins showing tissue specificity in their expression, which underlines the distinct biological functions of these two tissues within the developing barley grain.
authors.affiliation: Applied Biochemistry Group, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstrasse 3, 06466 Gatersleben, Germany.
authors.forename: Stephanie
authors.initials: S
authors.lastname: Kaspar
authors.forename: Diana
authors.initials: D
authors.lastname: Weier
authors.forename: Winfriede
authors.initials: W
authors.lastname: Weschke
authors.forename: Hans-Peter
authors.initials: HP
authors.lastname: Mock
authors.forename: Andrea
authors.initials: A
authors.lastname: Matros
mesh_terms: D001467:Hordeum / Q000737:chemistry* / Q000378:metabolism; D042282:Microdissection / Q000379:methods*; D010940:Plant Proteins / Q000032:analysis* / Q000302:isolation & purification; D012639:Seeds / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D010940:Plant Proteins