title: Development and validation of fixed-time method for the determination of isoxsuprine hydrochloride in commercial dosages forms.
abstract: The main aim of this work was to develop a kinetic spectrophotometric method for the quantitative analysis of isoxsuprine hydrochloride in commercial tablets. The method is based on the reaction of isoxsuprine hydrochloride (ISx) with hydroxylamine hydrochloride and ammonium cerium (IV) nitrate in sulphuric acid medium at room temperature which resulted in the formation of yellow-coloured product peaking at 380 nm. The reaction is followed spectrophotometrically by measuring the absorbance as a function of time. Fixed time method (ΔA = A₄-A₂, where A₂ and A₄ refer to absorbance measurements taken at 2 and 4 min, respectively) was adopted for constructing the calibration curve which was found to be linear over the concentration range of 30-80 µgmL⁻¹ with molar absorptivity of 5.95 × 10³ L mol⁻¹ cm⁻¹. The method has been applied successfully to the determination of isoxsuprine hydrochloride in tablets. Statistical comparison (point and interval hypothesis tests) of the results showed that there is no significant difference between the proposed method and reference method.
authors.affiliation: Department of Chemistry, Aligarh Muslim University, UP, India. cht17nr_amu@yahoo.com
authors.forename: Nafisur
authors.initials: N
authors.lastname: Rahman
authors.forename: Nasheed
authors.initials: N
authors.lastname: Afaq
mesh_terms: D002563:Cerium / Q000737:chemistry; D019811:Hydroxylamine / Q000737:chemistry; D007556:Isoxsuprine / Q000032:analysis*; D013053:Spectrophotometry / Q000379:methods; D013607:Tablets
chemical_list: D013607:Tablets; D019811:Hydroxylamine; D002563:Cerium; D007556:Isoxsuprine