Expression of RAD51, BRCA1 and P53 does not correlate with cellular radiosensitivity of normal human fibroblasts.
OBJECTIVE
To evaluate the potential role of key DNA repair proteins in the sensitivity of normal human fibroblasts to ionising radiations.


METHODS
Radiosensitivity of six human fibroblast strains established from skin biopsies of women who had undergone conservative breast surgery and received a curative breast conserving radiotherapy was measured by colony-formation assay. The expression level of RAD51, BRCA1 and p53 proteins were studied using western blot analysis.


RESULTS
The six fibroblast strains represent a typical spectrum of normal human radiosensitivity with the surviving fraction measured for a dose of 3.5 Gy (SF3.5) ranging from 0.21 to 0.40. We found that these differences in cell survival did not correlate with the expression of RAD51, BRCA1 nor p53 in the tested normal human fibroblast strains.


CONCLUSIONS
We conclude that measurement of protein expression of the three tested genes (RAD51, BRCA1 and p53) did not reflect sensitivity of normal fibroblasts to IR.
Saleh
E M Y
EM
Biochemistry and Molecular Biology Unit, Cancer Biology Department, National Cancer Institute, Cairo University, Cairo, Egypt. ekramsaleh@hotmail.com
El-Awady
R A E
RA
D019313:BRCA1 Protein / Q000378:metabolism*; D015153:Blotting, Western; D002470:Cell Survival; D004260:DNA Repair / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005347:Fibroblasts / Q000378:metabolism*; D016158:Genes, p53 / Q000502:physiology*; D006801:Humans; D015151:Immunoblotting; D010766:Phosphorylation; D051135:Rad51 Recombinase / Q000378:metabolism*; D011836:Radiation Tolerance
D019313:BRCA1 Protein; D051135:Rad51 Recombinase
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