title: The TCT motif, a key component of an RNA polymerase II transcription system for the translational machinery.
abstract: The TCT motif (polypyrimidine initiator) encompasses the transcription start site of nearly all ribosomal protein genes in Drosophila and mammals. The TCT motif is required for transcription of ribosomal protein gene promoters. The TCT element resembles the Inr (initiator), but is not recognized by TFIID and cannot function in lieu of an Inr. However, a single T-to-A substitution converts the TCT element into a functionally active Inr. Thus, the TCT motif is a novel transcriptional element that is distinct from the Inr. These findings reveal a specialized TCT-based transcription system that is directed toward the synthesis of ribosomal proteins.
authors.affiliation: Section of Molecular Biology, University of California at San Diego, La Jolla, California 92093, USA.
authors.forename: Trevor J
authors.initials: TJ
authors.lastname: Parry
authors.forename: Joshua W M
authors.initials: JW
authors.lastname: Theisen
authors.forename: Jer-Yuan
authors.initials: JY
authors.lastname: Hsu
authors.forename: Yuan-Liang
authors.initials: YL
authors.lastname: Wang
authors.forename: David L
authors.initials: DL
authors.lastname: Corcoran
authors.forename: Moriah
authors.initials: M
authors.lastname: Eustice
authors.forename: Uwe
authors.initials: U
authors.lastname: Ohler
authors.forename: James T
authors.initials: JT
authors.lastname: Kadonaga
mesh_terms: D001483:Base Sequence; D008969:Molecular Sequence Data; D012319:RNA Polymerase II / Q000378:metabolism*; D012269:Ribosomal Proteins / Q000096:biosynthesis / Q000235:genetics*; D016385:TATA Box; D035362:Transcription Factor TFIID / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000737:chemistry / Q000378:metabolism; D024363:Transcription Initiation Site; D014158:Transcription, Genetic
chemical_list: D012269:Ribosomal Proteins; D035362:Transcription Factor TFIID; D014157:Transcription Factors; D012319:RNA Polymerase II
references.citation: Dev Biol. 2010 Mar 15;339(2):225-9
references.pmid: 19682982
references.citation: Biochem Soc Trans. 2006 Feb;34(Pt 1):12-6
references.pmid: 16246169
references.citation: EMBO J. 1999 Sep 1;18(17):4835-45
references.pmid: 10469661
references.citation: Genes Dev. 2004 Jul 1;18(13):1606-17
references.pmid: 15231738
references.citation: Genome Biol. 2002;3(12):RESEARCH0087
references.pmid: 12537576
references.citation: Nucleic Acids Res. 1990 Oct 25;18(20):6097-100
references.pmid: 2172928
references.citation: Mol Cell Biol. 2010 Jul;30(14):3471-9
references.pmid: 20457814
references.citation: Proc Natl Acad Sci U S A. 1990 Feb;87(4):1526-30
references.pmid: 2304915
references.citation: Eur J Biochem. 2000 Nov;267(21):6321-30
references.pmid: 11029573
references.citation: Genes Dev. 2008 Sep 1;22(17):2353-8
references.pmid: 18703680
references.citation: Nat Methods. 2010 Jul;7(7):521-7
references.pmid: 20495556
references.citation: Genes Dev. 1997 Nov 15;11(22):3007-19
references.pmid: 9367983
references.citation: Mol Cell. 2008 Oct 10;32(1):96-105
references.pmid: 18851836
references.citation: Genes Dev. 2008 Oct 15;22(20):2823-30
references.pmid: 18923080
references.citation: EMBO J. 1994 Jul 1;13(13):3115-26
references.pmid: 7518774
references.citation: Genes Dev. 1994 Apr 1;8(7):830-42
references.pmid: 7926771
references.citation: Cell. 1995 Jun 30;81(7):1115-25
references.pmid: 7600579
references.citation: Gene. 2006 Jan 3;365:48-56
references.pmid: 16343812
references.citation: Genes Dev. 1988 Jan;2(1):68-81
references.pmid: 3356339
references.citation: Mol Cell. 2005 May 13;18(4):471-81
references.pmid: 15893730
references.citation: Nucleic Acids Res. 2004 Jan 1;32(Database issue):D168-70
references.pmid: 14681386
references.citation: Science. 2000 Nov 3;290(5493):982-5
references.pmid: 11062130
references.citation: Gene. 1991 Apr;100:173-80
references.pmid: 2055468
references.citation: Genes Dev. 1996 Mar 15;10(6):711-24
references.pmid: 8598298
references.citation: BMC Evol Biol. 2005 Feb 13;5:15
references.pmid: 15707503
references.citation: Nucleic Acids Res. 2009 Jan;37(Database issue):D49-53
references.pmid: 18842623
references.citation: J Biol Chem. 1990 Dec 5;265(34):21223-31
references.pmid: 2250021
references.citation: Genes Dev. 2007 Sep 1;21(17):2137-49
references.pmid: 17704303
references.citation: Genes Dev. 1994 Apr 1;8(7):821-9
references.pmid: 7926770
references.citation: Genome Res. 2004 Jun;14(6):1188-90
references.pmid: 15173120
references.citation: Annu Rev Biochem. 2003;72:449-79
references.pmid: 12651739
references.citation: Mol Cell. 2009 Dec 25;36(6):924-31
references.pmid: 20064459
references.citation: Development. 2010 Jan;137(1):15-26
references.pmid: 20023156