title: Determination of anthocyanins in the urine of patients with colorectal liver metastases after administration of bilberry extract.
abstract: Anthocyanins possess cancer chemopreventive properties in preclinical models. Their clinical pharmacology is only poorly understood. In this pilot study, anthocyanins and their metabolites were analysed in the urine of two patients with colorectal liver metastases. They received a single dose of 1.88 g standardized bilberry extract (mirtoselect) via either nasogastric or nasojejunal tube intra-operatively during liver resection. HPLC-MS/MS and HPLC-UV analysis showed there were more anthocyanins and metabolites in the urine of the patient who received mirtoselect via the stomach than via the jejunum. This result is consistent with information obtained in rodents which suggests the stomach is the predominant site for anthocyanin absorption.
authors.affiliation: Cancer Biomarkers and Prevention Group, Department of Cancer Studies and Molecular Medicine, University of Leicester, UK. hc47@le.ac.uk
authors.forename: Hong
authors.initials: H
authors.lastname: Cai
authors.forename: Sarah C
authors.initials: SC
authors.lastname: Thomasset
authors.forename: David
authors.initials: D
authors.lastname: P-Berry
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Garcea
authors.forename: Karen
authors.initials: K
authors.lastname: Brown
authors.forename: William P
authors.initials: WP
authors.lastname: Steward
authors.forename: Andreas J
authors.initials: AJ
authors.lastname: Gescher
mesh_terms: D000368:Aged; D000872:Anthocyanins / Q000737:chemistry / Q000302:isolation & purification / Q000652:urine*; D000970:Antineoplastic Agents / Q000008:administration & dosage; D002851:Chromatography, High Pressure Liquid; D015179:Colorectal Neoplasms / Q000188:drug therapy / Q000473:pathology / Q000601:surgery / Q000652:urine*; D006801:Humans; D007441:Intubation, Gastrointestinal; D008113:Liver Neoplasms / Q000188:drug therapy / Q000556:secondary / Q000601:surgery / Q000652:urine*; D008297:Male; D010865:Pilot Projects; D010936:Plant Extracts / Q000008:administration & dosage*; D053719:Tandem Mass Spectrometry; D029800:Vaccinium myrtillus / Q000737:chemistry*
chemical_list: D000872:Anthocyanins; D000970:Antineoplastic Agents; D010936:Plant Extracts