Extracts of edible and medicinal plants damage membranes of Vibrio cholerae.
The use of natural compounds from plants can provide an alternative approach against food-borne pathogens. The mechanisms of action of most plant extracts with antimicrobial activity have been poorly studied. In this work, changes in membrane integrity, membrane potential, internal pH (pH(in)), and ATP synthesis were measured in Vibrio cholerae cells after exposure to extracts of edible and medicinal plants. A preliminary screen of methanolic, ethanolic, and aqueous extracts of medicinal and edible plants was performed. Minimal bactericidal concentrations (MBCs) were measured for extracts showing high antimicrobial activity. Our results indicate that methanolic extracts of basil (Ocimum basilicum L.), nopal cactus (Opuntia ficus-indica var. Villanueva L.), sweet acacia (Acacia farnesiana L.), and white sagebrush (Artemisia ludoviciana Nutt.) are the most active against V. cholera, with MBCs ranging from 0.5 to 3.0 mg/ml. Using four fluorogenic techniques, we studied the membrane integrity of V. cholerae cells after exposure to these four extracts. Extracts from these plants were able to disrupt the cell membranes of V. cholerae cells, causing increased membrane permeability, a clear decrease in cytoplasmic pH, cell membrane hyperpolarization, and a decrease in cellular ATP concentration in all strains tested. These four plant extracts could be studied as future alternatives to control V. cholerae contamination in foods and the diseases associated with this microorganism.
Sánchez
Eduardo
E
Departamento de Microbiología e Inmunología, Universidad Autónoma de Nuevo León, Apdo. Postal 124-F, Ciudad Universitaria, San Nicolás de los Garza N.L. 66451, México.
García
Santos
S
Heredia
Norma
N
D000255:Adenosine Triphosphate / Q000096:biosynthesis; D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects*; D003593:Cytoplasm / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008564:Membrane Potentials / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability / Q000187:drug effects; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D010945:Plants, Edible / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D014734:Vibrio cholerae / Q000187:drug effects*
D000900:Anti-Bacterial Agents; D010936:Plant Extracts; D000255:Adenosine Triphosphate
Appl Environ Microbiol. 2003 Dec;69(12):7401-8
14660391
Food Chem. 2008 Jun 1;108(3):986-95
26065762
Anal Sci. 2003 Sep;19(9):1239-42
14516073
Appl Environ Microbiol. 2004 Nov;70(11):6884-6
15528557
J Bacteriol. 2003 Sep;185(18):5527-35
12949105
Lett Appl Microbiol. 1998 May;26(5):355-8
9674165
J Appl Microbiol. 2000 May;88(5):809-16
10792541
Eur Biophys J. 2009 Oct;38(8):1049-59
19579022
Microbiol Rev. 1995 Jun;59(2):201-22
7603409
BMC Complement Altern Med. 2006 Nov 30;6:39
17134518
Pol J Microbiol. 2009;58(1):69-76
19469289
J Food Prot. 2002 Dec;65(12):1909-15
12495009
Appl Environ Microbiol. 1996 Jan;62(1):178-83
16535209
Appl Environ Microbiol. 2007 May;73(10):3283-90
17384309
J Appl Bacteriol. 1995 Mar;78(3):309-15
7537262
J Biomol Screen. 2001 Oct;6(5):305-12
11689130
Appl Environ Microbiol. 1996 Aug;62(8):2701-9
16535371
J Antimicrob Chemother. 2004 Feb;53(2):230-9
14729742
Antimicrob Agents Chemother. 1997 Sep;41(9):2001-5
9303401
J Food Prot. 2008 Jul;71(7):1379-85
18680936
Appl Environ Microbiol. 1996 Sep;62(9):3158-64
8795204
J Appl Microbiol. 2001 Sep;91(3):453-62
11556910
J Food Prot. 2005 Jul;68(7):1346-51
16013369
Nature. 2003 Oct 30;425(6961):980-4
14586471
Mol Microbiol. 1993 Apr;8(1):5-14
8388532
Clin Microbiol Rev. 1999 Oct;12(4):564-82
10515903
J Bacteriol. 1981 Sep;147(3):820-6
6268609
Arch Pharm Res. 2003 Dec;26(12):1018-23
14723334
Appl Environ Microbiol. 1999 Oct;65(10):4606-10
10508096
Appl Environ Microbiol. 2005 Mar;71(3):1662-3
15746375
J Food Prot. 2006 Dec;69(12):2961-9
17186665
Appl Environ Microbiol. 2002 Apr;68(4):1561-8
11916669