title: Detection of Mycobacterium avium subsp. paratuberculosis in drinking water and biofilms by quantitative PCR.
abstract: It has been suggested that Mycobacterium avium subspecies paratuberculosis has a role in Crohn's disease. The organism may be acquired but is difficult to culture from the environment. We describe a quantitative PCR (qPCR) method to detect M. avium subsp. paratuberculosis in drinking water and the results of its application to drinking water and faucet biofilm samples collected in the United States.
authors.affiliation: University of Cincinnati, Raymond Walters College, Blue Ash, Ohio 45236, USA.
authors.forename: Amy
authors.initials: A
authors.lastname: Beumer
authors.forename: Dawn
authors.initials: D
authors.lastname: King
authors.forename: Maura
authors.initials: M
authors.lastname: Donohue
authors.forename: Jatin
authors.initials: J
authors.lastname: Mistry
authors.forename: Terry
authors.initials: T
authors.lastname: Covert
authors.forename: Stacy
authors.initials: S
authors.lastname: Pfaller
mesh_terms: D018441:Biofilms; D004269:DNA, Bacterial / Q000235:genetics; D016927:Mycobacterium avium subsp. paratuberculosis / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D014481:United States; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial
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