title: Auxin carriers localization drives auxin accumulation in plant cells infected by Frankia in Casuarina glauca actinorhizal nodules.
abstract: Actinorhizal symbioses are mutualistic interactions between plants and the soil bacteria Frankia that lead to the formation of nitrogen-fixing root nodules. Little is known about the signaling mechanisms controlling the different steps of the establishment of the symbiosis. The plant hormone auxin has been suggested to play a role. Here we report that auxin accumulates within Frankia-infected cells in actinorhizal nodules of Casuarina glauca. Using a combination of computational modeling and experimental approaches, we establish that this localized auxin accumulation is driven by the cell-specific expression of auxin transporters and by Frankia auxin biosynthesis in planta. Our results indicate that the plant actively restricts auxin accumulation to Frankia-infected cells during the symbiotic interaction.
authors.affiliation: UMR DIAPC, Institut de Recherche pour le Développement, Montpellier cedex 5, France.
authors.forename: Francine
authors.initials: F
authors.lastname: Perrine-Walker
authors.forename: Patrick
authors.initials: P
authors.lastname: Doumas
authors.forename: Mikael
authors.initials: M
authors.lastname: Lucas
authors.forename: Virginie
authors.initials: V
authors.lastname: Vaissayre
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: Beauchemin
authors.forename: Leah R
authors.initials: LR
authors.lastname: Band
authors.forename: Jérome
authors.initials: J
authors.lastname: Chopard
authors.forename: Amandine
authors.initials: A
authors.lastname: Crabos
authors.forename: Geneviève
authors.initials: G
authors.lastname: Conejero
authors.forename: Benjamin
authors.initials: B
authors.lastname: Péret
authors.forename: John R
authors.initials: JR
authors.lastname: King
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Verdeil
authors.forename: Valérie
authors.initials: V
authors.lastname: Hocher
authors.forename: Claudine
authors.initials: C
authors.lastname: Franche
authors.forename: Malcolm J
authors.initials: MJ
authors.lastname: Bennett
authors.forename: Louis S
authors.initials: LS
authors.lastname: Tisa
authors.forename: Laurent
authors.initials: L
authors.lastname: Laplaze
mesh_terms: D002352:Carrier Proteins / Q000378:metabolism; D019295:Computational Biology; D040161:Frankia; D020869:Gene Expression Profiling; D007210:Indoleacetic Acids / Q000378:metabolism*; D019684:Magnoliopsida / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology; D008954:Models, Biological; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000378:metabolism; D053204:Root Nodules, Plant / Q000378:metabolism*; D013559:Symbiosis
chemical_list: D002352:Carrier Proteins; D007210:Indoleacetic Acids; D010940:Plant Proteins
references.citation: J Bacteriol. 2008 Dec;190(23):7864-7
references.pmid: 18820019
references.citation: Can J Microbiol. 1996 Mar;42(3):207-20
references.pmid: 8868227
references.citation: New Phytol. 2006;169(4):681-8
references.pmid: 16441749
references.citation: Genome Res. 2007 Jan;17(1):7-15
references.pmid: 17151343
references.citation: Curr Opin Plant Biol. 2007 Aug;10(4):372-9
references.pmid: 17646123
references.citation: Plant Cell. 2009 Sep;21(9):2553-62
references.pmid: 19789282
references.citation: Annu Rev Plant Biol. 2010;61:65-87
references.pmid: 20192756
references.citation: FEMS Microbiol Rev. 2007 Jul;31(4):425-48
references.pmid: 17509086
references.citation: Plant Physiol. 2007 Aug;144(4):1852-62
references.pmid: 17556507
references.citation: Science. 2009 May 8;324(5928):753-4
references.pmid: 19423817
references.citation: Plant Cell. 1997 Nov;9(11):1963-71
references.pmid: 9401121
references.citation: Plant J. 1998 Apr;14(1):23-34
references.pmid: 15494052
references.citation: Genes Dev. 2001 Oct 15;15(20):2648-53
references.pmid: 11641271
references.citation: Plant Cell. 1996 Oct;8(10):1899-1913
references.pmid: 12239369
references.citation: Plant Mol Biol. 1991 Sep;17(3):567-79
references.pmid: 1884011
references.citation: Microbiol Rev. 1993 Jun;57(2):293-319
references.pmid: 8336669
references.citation: Funct Plant Biol. 2008 Dec;35(10):751-760
references.pmid: 32688829
references.citation: BMC Plant Biol. 2007 May 08;7:21
references.pmid: 17488509
references.citation: Mol Gen Genet. 1998 Oct;260(1):115-9
references.pmid: 9829835
references.citation: Arch Microbiol. 2008 Jul;190(1):67-77
references.pmid: 18415080
references.citation: Funct Plant Biol. 2008 Oct;35(8):651-668
references.pmid: 32688821
references.citation: Trends Plant Sci. 2009 Jul;14(7):373-82
references.pmid: 19559643
references.citation: Plant Mol Biol. 2003 Aug;52(6):1169-80
references.pmid: 14682616
references.citation: Curr Opin Plant Biol. 2006 Dec;9(6):621-30
references.pmid: 17011813
references.citation: Proc Natl Acad Sci U S A. 2006 Jan 31;103(5):1627-32
references.pmid: 16432202
references.citation: Proc Natl Acad Sci U S A. 2007 Dec 11;104(50):20131-6
references.pmid: 18056646
references.citation: Nat Cell Biol. 2008 Aug;10(8):946-54
references.pmid: 18622388
references.citation: Proc Natl Acad Sci U S A. 2008 Mar 25;105(12):4928-32
references.pmid: 18316735
references.citation: Plant Signal Behav. 2008 Jan;3(1):34-5
references.pmid: 19704764
references.citation: Plant Physiol. 2006 Sep;142(1):254-64
references.pmid: 16844839
references.citation: Nucleic Acids Res. 2000 Jan 1;28(1):27-30
references.pmid: 10592173
references.citation: Mol Cells. 2007 Feb 28;23(1):115-21
references.pmid: 17464220
references.citation: Methods Mol Biol. 2000;141:49-65
references.pmid: 10820736
references.citation: Planta. 2003 Mar;216(5):841-53
references.pmid: 12624772