title: MKP1 regulates the induction of MCP1 by Streptococcus pneumoniae pneumolysin in human epithelial cells.
abstract: Epithelial cells act as the first line of host defense against microbes by producing a range of different molecules for clearance. Chemokines facilitate the clearance of invaders through the recruitment of leukocytes. Thus, upregulation of chemokine expression represents an important innate host defense response against invading microbes such as Streptococcus pneumoniae. In this study, we report that the expression of Monocyte Chemotactic Protein 1 (MCP1) was highly induced in response to S. pneumoniae in vitro and in vivo. Among numerous virulence factors, pneumococcal pneumolysin was found to be the major factor responsible for this induction. Furthermore, MCP1 induction was mediated by the p38 mitogen-activated protein kinase (MAPK) whose activation was controlled by MAPK phosphatase 1 (MKP1). Therefore, this study reveals novel roles of pneumolysin in mediating MKP1 expression for the regulation of MCP1 expression in human epithelial cells.
authors.affiliation: Department of Biotechnology and Bioinformatics, Korea University, Yeongi, 339-700, Korea.
authors.forename: Hee-Sung
authors.initials: HS
authors.lastname: Shin
authors.forename: In-Hwa
authors.initials: IH
authors.lastname: Yoo
authors.forename: Yong-Jae
authors.initials: YJ
authors.lastname: Kim
authors.forename: Ji-Yun
authors.initials: JY
authors.lastname: Lee
authors.forename: Hyong-Bai
authors.initials: HB
authors.lastname: Kim
authors.forename: Shouguang
authors.initials: S
authors.lastname: Jin
authors.forename: Un-Hwan
authors.initials: UH
authors.lastname: Ha
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism; D002460:Cell Line; D018932:Chemokine CCL2 / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D054638:Dual Specificity Phosphatase 1 / Q000378:metabolism*; D004847:Epithelial Cells / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology; D005786:Gene Expression Regulation; D006801:Humans; D007113:Immunity, Innate; D051379:Mice; D008810:Mice, Inbred C57BL; D034741:RNA, Small Interfering / Q000235:genetics; D013290:Streptococcal Infections / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D013296:Streptococcus pneumoniae / Q000276:immunology* / Q000472:pathogenicity; D013301:Streptolysins / Q000378:metabolism; D014774:Virulence; D048051:p38 Mitogen-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; C111835:CCL2 protein, human; D018932:Chemokine CCL2; D034741:RNA, Small Interfering; D013301:Streptolysins; C032290:plY protein, Streptococcus pneumoniae; D048051:p38 Mitogen-Activated Protein Kinases; D054638:Dual Specificity Phosphatase 1