Chondroitin sulfate N-acetylgalactosaminyltransferase-1 is required for normal cartilage development.
CS (chondroitin sulfate) is a glycosaminoglycan species that is widely distributed in the extracellular matrix. To understand the physiological roles of enzymes involved in CS synthesis, we produced CSGalNAcT1 (CS N-acetylgalactosaminyltransferase 1)-null mice. CS production was reduced by approximately half in CSGalNAcT1-null mice, and the amount of short-chain CS was also reduced. Moreover, the cartilage of the null mice was significantly smaller than that of wild-type mice. Additionally, type-II collagen fibres in developing cartilage were abnormally aggregated and disarranged in the homozygous mutant mice. These results suggest that CSGalNAcT1 is required for normal CS production in developing cartilage.
Watanabe
Yumi
Y
Niigata University, Chuo-ku, Japan.
Takeuchi
Kosei
K
Higa Onaga
Susumu
S
Sato
Michiko
M
Tsujita
Mika
M
Abe
Manabu
M
Natsume
Rie
R
Li
Minqi
M
Furuichi
Tatsuya
T
Saeki
Mika
M
Izumikawa
Tomomi
T
Hasegawa
Ayumi
A
Yokoyama
Minesuke
M
Ikegawa
Shiro
S
Sakimura
Kenji
K
Amizuka
Norio
N
Kitagawa
Hiroshi
H
Igarashi
Michihiro
M
D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D049109:Cell Proliferation; D019902:Chondrocytes / Q000166:cytology / Q000201:enzymology / Q000378:metabolism; D020219:Chondrogenesis; D002809:Chondroitin Sulfates / Q000096:biosynthesis*; D024043:Collagen Type II / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D006132:Growth Plate / Q000196:embryology / Q000201:enzymology* / Q000378:metabolism; D007150:Immunohistochemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D017350:N-Acetylgalactosaminyltransferases / Q000235:genetics / Q000378:metabolism*; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
D024043:Collagen Type II; D018809:Proliferating Cell Nuclear Antigen; D002809:Chondroitin Sulfates; D017350:N-Acetylgalactosaminyltransferases; C569943:chondroitin N-acetylgalactosaminyltransferase-1, mouse; C472559:chondroitin sulfate N-acetylgalactosaminyltransferase-1
J Biol Chem. 2007 Feb 9;282(6):4152-61
17145758
Bone. 2004 Jan;34(1):13-25
14751559
Folia Histochem Cytobiol. 2009;47(1):5-16
19419931
J Biol Chem. 2008 Apr 25;283(17):11396-406
18316376
J Biol Chem. 2003 Jan 31;278(5):3072-8
12433924
Biochem Biophys Res Commun. 2002 May 10;293(3):953-7
12051751
J Biochem. 1998 Jun;123(6):1000-9
9603985
J Biol Chem. 2003 Nov 28;278(48):47534-44
12966086
Annu Rev Biochem. 2008;77:521-55
18518825
J Biol Chem. 2006 Jul 7;281(27):18444-50
16679517
Development. 2009 May;136(10):1697-706
19369399
Biochem Biophys Res Commun. 2004 Jun 4;318(3):665-72
15144889
Nat Med. 2007 Nov;13(11):1363-7
17952091
J Biol Chem. 2002 Mar 15;277(11):8841-6
11788602
Biochem J. 2007 May 1;403(3):545-52
17253960
J Biol Chem. 2002 Oct 11;277(41):38189-96
12163485
Tissue Eng Part B Rev. 2009 Mar;15(1):29-41
19063663
Biochem J. 2009 Jun 26;421(2):157-62
19473117
Eur J Neurosci. 2001 Jan;13(1):179-89
11135016
Anal Biochem. 1999 May 1;269(2):367-78
10222012
Glycobiology. 2002 Apr;12(4):57R-68R
12042245
J Biol Chem. 2003 Jun 27;278(26):23666-71
12716890
J Neurosci. 1999 Dec 1;19(23):10318-23
10575029
Chem Biol Drug Des. 2008 Dec;72(6):455-82
19090915
Trends Biochem Sci. 2005 Jan;30(1):53-62
15653326
Biochem Biophys Res Commun. 2001 Mar;281(5):1134-40
11243853
Biochem J. 2001 Feb 15;354(Pt 1):199-207
11171095
Curr Opin Struct Biol. 2003 Oct;13(5):612-20
14568617
Neurosci Res. 2007 Jun;58(2):105-12
17298852
J Biol Chem. 2001 Oct 19;276(42):38721-6
11514575