A highly soluble poly(3,4-ethylenedioxythiophene)-poly(styrene sulfonic acid)/Au nanocomposite for horseradish peroxidase immobilization and biosensing.
A highly soluble poly(3,4-ethylenedioxythiophene)-poly(styrene sulfonic acid)/Au (PEDOT-PSS/Au) nanocomposite was prepared via one-step chemical synthesis and the matrix was characterized by UV-vis spectroscopy (UV-vis), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM) and transmission electron microscope (TEM). Due to the excellent aqueous compatibility and biocompatibility, the PEDOT/PSS-Au nanocomposite can be used as biomaterial for enzymes immobilization. In this system, redox enzyme, horseradish peroxidase (HRP) was integrated with PEDOT/PSS-Au nanocomposite and the direct electron transfer of HRP was observed. Moreover, we find that the HRP/PEDOT-PSS/Au modified electrode shows excellent electrocatalytic ability for H(2)O(2) and the formal Michaelis-Menten constant (K(m)(app)) was 0.78 mmol/L. The response currents have good linear relation with the concentrations of H(2)O(2) with a linear range from 2.0 x 10(-7) to 3.8 x 10(-4)mol/L.
Xu
Jingjing
J
Department of Chemistry, East China Normal University, 3663 Zhongshan N Road, Shanghai 200062, China.
Peng
Ru
R
Ran
Qin
Q
Xian
Yuezhong
Y
Tian
Yuan
Y
Jin
Litong
L
D015374:Biosensing Techniques; D004800:Enzymes, Immobilized / Q000378:metabolism*; D006046:Gold; D006735:Horseradish Peroxidase / Q000378:metabolism*; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D053761:Nanocomposites; D011137:Polystyrenes / Q000737:chemistry*; D012995:Solubility; D013056:Spectrophotometry, Ultraviolet; D013876:Thiophenes / Q000737:chemistry*
D004800:Enzymes, Immobilized; D011137:Polystyrenes; D013876:Thiophenes; C533756:poly(3,4-ethylenedioxythiophene)-poly(styrenesulfonate); D006046:Gold; D006735:Horseradish Peroxidase