Insulator base pairs for lighting-up perylenediimide in a DNA duplex.
Quenched: Perylenediimide (PDI) is highly quenched by nucleobases, which greatly restricts its application as a fluorescent probe. Here, we propose "insulator base pairs" tethering cyclohexane ring through D-threoninol. When "insulator base pairs" were inserted between PDI and nucleobases, the quantum yield of PDI drastically increased several thousand-fold. The "insulator base pairs" reported here also have the potential to increase the quantum yields of other fluorophores.
Kashida
Hiromu
H
Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.
Sekiguchi
Koji
K
Asanuma
Hiroyuki
H
D020029:Base Pairing; D003510:Cyclohexanes / Q000737:chemistry; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D005456:Fluorescent Dyes; D007094:Imides / Q000737:chemistry*; D010569:Perylene / Q000031:analogs & derivatives* / Q000737:chemistry; D011789:Quantum Theory; D044366:Transition Temperature
D003510:Cyclohexanes; D005456:Fluorescent Dyes; D007094:Imides; C521332:perylenediimide; C506365:Cyclohexane; D010569:Perylene; D004247:DNA