title: Redox modulation of the fetal cardiovascular defence to hypoxaemia.
abstract: Episodes of hypoxia in utero present a potentially serious challenge to the fetus, but are counteracted by defence responses including marked redistribution of blood flow from peripheral circulations to the brain. Here, we report the novel observation that the oxidant tone is an important modulator of this cardiovascular defence. Using pregnant Welsh Mountain sheep surgically prepared for long-term recording, we investigated in vivo the effects on the fetal cardiovascular defence to acute hypoxaemia of fetal treatment with the antioxidant vitamin C. The mechanisms via which vitamin C may affect the vascular oxidant tone were investigated by monitoring fetal plasma concentrations of nitrates and nitrites, by determining changes in the activity of superoxide dismutase (SOD) in fetal plasma, and by investigating the effect of vitamin C treatment on the fetal cardiovascular defence to hypoxaemia following nitric oxide (NO) synthase blockade. Fetal treatment with vitamin C markedly depressed the normal femoral constrictor response to acute hypoxaemia in the fetus (5.2 ± 1.0 vs. 1.1 ± 0.3 mmHg (ml min(-1))(-1), mean ± s.e.m.; P < 0.05) an effect which was completely restored following NO synthase blockade (6.2 ± 1.3 mmHg (ml min(-1))(-1)). Compared to saline infusion, fetal treatment with vitamin C during acute hypoxaemia also significantly increased fetal plasma SOD activity from normoxic baseline (-8.9 ± 6.5 vs. 15.0 ± 6.6% inhibition, P < 0.05) and decreased the plasma concentration ratio of nitrate:nitrite from normoxic baseline (ΔNO3(-):NO2(-); 0.15 ± 0.30 vs. -0.29 ± 0.11, P < 0.05). The data provide in vivo evidence of redox modulation of redistribution of blood flow in the fetus, part of the fetal brain sparing during acute hypoxaemic stress.
authors.affiliation: Department of Physiology, Development and Neuroscience, University of Cambridge, Downing Street, Cambridge CB2 3EG, UK.
authors.forename: A S
authors.initials: AS
authors.lastname: Thakor
authors.forename: H G
authors.initials: HG
authors.lastname: Richter
authors.forename: A D
authors.initials: AD
authors.lastname: Kane
authors.forename: C
authors.initials: C
authors.lastname: Dunster
authors.forename: F J
authors.initials: FJ
authors.lastname: Kelly
authors.forename: L
authors.initials: L
authors.lastname: Poston
authors.forename: D A
authors.initials: DA
authors.lastname: Giussani
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use; D001205:Ascorbic Acid / Q000097:blood / Q000627:therapeutic use; D001784:Blood Gas Analysis; D002319:Cardiovascular System / Q000503:physiopathology*; D005260:Female; D005333:Fetus / Q000502:physiology*; D000860:Hypoxia / Q000188:drug therapy / Q000378:metabolism / Q000503:physiopathology*; D023421:Models, Animal; D009566:Nitrates / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D009573:Nitrites / Q000378:metabolism; D010084:Oxidation-Reduction; D011247:Pregnancy; D012756:Sheep; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D009566:Nitrates; D009573:Nitrites; D009569:Nitric Oxide; D013482:Superoxide Dismutase; D001205:Ascorbic Acid
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