title: [Effects of ingredients from Chinese herbs with nature of cold or hot on expression of TRPV1 and TRPM8].
abstract: OBJECTIVE
  To study the effects of the ingredients from Chinese herbs with the nature of cold or hot on the expression of TRPV1 and TRPM8.
  
  
  METHODS
  The effects of ingredients from herbs on primary culture DRG neurons are observed in vitro. The expression quantity of gene is detected by the method of real time PCR. the 2 (-deltadeltaCT) method is applied to analyze the data.
  
  
  RESULTS
  Ingredients from herbs with the nature of cold up-regulate the expression level of TRPV1 and down-regulate that of TRPM8, especially under the temperature condition of 39 degrees C; while ingredients from herbs with the nature of hot up-regulate the expression level of TRPM8 and down-regulated that of TRPV1, which is more significant under the temperature condition of 19 degrees C.
  
  
  CONCLUSIONS
  The regulatory changes of TRPV1 and TRPM8 mRNA expression induced by the chemical ingredients might be related to the cold and hot natures of the herbs from which the ingredients are extracted. And this could be one of the therapeutic mechanisms for the treatment of Chinese herbal medicines to cold- and heat-related diseases.
authors.affiliation: Tang Center for Herbal Medicine Research, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Sui
authors.forename: Na
authors.initials: N
authors.lastname: Yang
authors.forename: Changbin
authors.initials: C
authors.lastname: Zhang
authors.forename: Xinliang
authors.initials: X
authors.lastname: Du
authors.forename: Lanfang
authors.initials: L
authors.lastname: Li
authors.forename: Xiaogang
authors.initials: X
authors.lastname: Weng
authors.forename: Shuying
authors.initials: S
authors.lastname: Guo
authors.forename: Hairu
authors.initials: H
authors.lastname: Huo
authors.forename: Tingliang
authors.initials: T
authors.lastname: Jiang
mesh_terms: D000818:Animals; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage* / Q000032:analysis; D015870:Gene Expression / Q000187:drug effects*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D050053:TRPM Cation Channels / Q000235:genetics* / Q000378:metabolism; D050916:TRPV Cation Channels / Q000235:genetics* / Q000378:metabolism
chemical_list: D004365:Drugs, Chinese Herbal; D050053:TRPM Cation Channels; D050916:TRPV Cation Channels; C498947:Trpm8 protein, rat; C493922:Trpv1 protein, rat