title: A commensal gone bad: complete genome sequence of the prototypical enterotoxigenic Escherichia coli strain H10407.
abstract: In most cases, Escherichia coli exists as a harmless commensal organism, but it may on occasion cause intestinal and/or extraintestinal disease. Enterotoxigenic E. coli (ETEC) is the predominant cause of E. coli-mediated diarrhea in the developing world and is responsible for a significant portion of pediatric deaths. In this study, we determined the complete genomic sequence of E. coli H10407, a prototypical strain of enterotoxigenic E. coli, which reproducibly elicits diarrhea in human volunteer studies. We performed genomic and phylogenetic comparisons with other E. coli strains, revealing that the chromosome is closely related to that of the nonpathogenic commensal strain E. coli HS and to those of the laboratory strains E. coli K-12 and C. Furthermore, these analyses demonstrated that there were no chromosomally encoded factors unique to any sequenced ETEC strains. Comparison of the E. coli H10407 plasmids with those from several ETEC strains revealed that the plasmids had a mosaic structure but that several loci were conserved among ETEC strains. This study provides a genetic context for the vast amount of experimental and epidemiological data that have been published.
authors.affiliation: The Wellcome Trust Sanger Institute, Genome Campus, Hinxton, Cambridge, United Kingdom.
authors.forename: Lisa C
authors.initials: LC
authors.lastname: Crossman
authors.forename: Roy R
authors.initials: RR
authors.lastname: Chaudhuri
authors.forename: Scott A
authors.initials: SA
authors.lastname: Beatson
authors.forename: Timothy J
authors.initials: TJ
authors.lastname: Wells
authors.forename: Mickael
authors.initials: M
authors.lastname: Desvaux
authors.forename: Adam F
authors.initials: AF
authors.lastname: Cunningham
authors.forename: Nicola K
authors.initials: NK
authors.lastname: Petty
authors.forename: Vivienne
authors.initials: V
authors.lastname: Mahon
authors.forename: Carl
authors.initials: C
authors.lastname: Brinkley
authors.forename: Jon L
authors.initials: JL
authors.lastname: Hobman
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Savarino
authors.forename: Susan M
authors.initials: SM
authors.lastname: Turner
authors.forename: Mark J
authors.initials: MJ
authors.lastname: Pallen
authors.forename: Charles W
authors.initials: CW
authors.lastname: Penn
authors.forename: Julian
authors.initials: J
authors.lastname: Parkhill
authors.forename: A Keith
authors.initials: AK
authors.lastname: Turner
authors.forename: Timothy J
authors.initials: TJ
authors.lastname: Johnson
authors.forename: Nicholas R
authors.initials: NR
authors.lastname: Thomson
authors.forename: Stephen G J
authors.initials: SG
authors.lastname: Smith
authors.forename: Ian R
authors.initials: IR
authors.lastname: Henderson
mesh_terms: D000595:Amino Acid Sequence; D002876:Chromosomes, Bacterial; D054307:Enterotoxigenic Escherichia coli / Q000145:classification* / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D036781:Fimbriae Proteins; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D016680:Genome, Bacterial; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics
chemical_list: D029968:Escherichia coli Proteins; D036781:Fimbriae Proteins
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