RLK7, a leucine-rich repeat receptor-like kinase, is required for proper germination speed and tolerance to oxidative stress in Arabidopsis thaliana.
The leucine-rich repeat class of receptor-like kinase (LRR-RLKs) encoding genes represents the largest family of putative receptor genes in the Arabidopsis thaliana genome. However, very little is known about the range of biological process that they control. We present in this paper the functional characterization of RLK7 that has all the structural features of a receptor-like kinase of the plant-specific LRR type. To this end, we identified and characterized three independent T-DNA insertion mutants, constructed lines carrying truncated versions of this putative receptor, one lacking the cytoplasmic kinase domain (RLK7Δkin) and the other one lacking 14 LRR repeats (RLK7ΔLRR) and generated RLK7 overexpressing lines. We thus provide evidences that RLK7 is involved in the control of germination speed and the tolerance to oxidant stress. First, consistent with the expression kinetics of the RLK7 gene in the seeds, we found that all three mutants showed a delay in germination, whereas the overexpressors, RLK7Δkin and RLK7ΔLRR lines displayed a phenotype of more precocious germination. Second, a non-hypothesis driven proteomic approach revealed that in the seedlings of the three T-DNA insertion lines, four enzymes directly or indirectly involved in reactive oxygen species detoxification, were significantly less abundant. Consistent with this finding, the three mutants were less tolerant than the wild type to a hydrogen peroxide treatment, whereas the overexpressors, RLK7Δkin and RLK7ΔLRR lines presented the opposite phenotype.
Pitorre
Delphine
D
Laboratoire Génome et Développement des Plantes, UMR 5096 CNRS-UPVD-IRD, Université de Perpignan, 58 Avenue Paul Alduy, 66860, Perpignan Cedex, France. delphine.pitorre@univie.ac.at
Llauro
Christel
C
Jobet
Edouard
E
Guilleminot
Jocelyne
J
Brizard
Jean-Paul
JP
Delseny
Michel
M
Lasserre
Eric
E
D000222:Adaptation, Physiological; D017360:Arabidopsis / Q000201:enzymology / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D018525:Germination; D006861:Hydrogen Peroxide / Q000378:metabolism; D017403:In Situ Hybridization; D007930:Leucine / Q000378:metabolism*; D009154:Mutation; D018384:Oxidative Stress; D011401:Promoter Regions, Genetic; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D029681:Arabidopsis Proteins; D006861:Hydrogen Peroxide; D007930:Leucine
Development. 2001 Jan;128(2):243-52
11124119
Plant Physiol. 2003 Jun;132(2):530-43
12805585
Mol Plant Microbe Interact. 2008 May;21(5):507-17
18393610
Trends Plant Sci. 2003 May;8(5):231-7
12758041
C R Biol. 2008 Oct;331(10):806-14
18926495
Annu Rev Cell Dev Biol. 2002;18:163-92
12142267
Plant J. 1996 Feb;9(2):195-203
8820606
Curr Opin Plant Biol. 2006 Oct;9(5):460-9
16877029
Proteomics. 2007 Jun;7(11):1908-15
17464940
Plant Cell. 2006 Jul;18(7):1667-80
16751349
Development. 2003 Dec;130(24):6065-73
14597573
Plant J. 2008 Apr;54(1):30-42
18088309
Plant J. 2002 May;30(3):373-83
12000684
Nat Cell Biol. 2009 Oct;11(10):1166-73
19794500
Plant J. 1998 Dec;16(6):735-43
10069079
Plant J. 1996 Jan;9(1):101-24
8580968
Sci STKE. 2001 Dec 18;2001(113):re22
11752632
Plant J. 2007 May;50(3):452-65
17376157
Annu Rev Plant Biol. 2009;60:379-406
19400727
Plant Physiol. 2009 Aug;150(4):1855-65
19493972
Plant Cell. 1997 Jul;9(7):1055-1066
12237375
Plant Cell. 1994 Dec;6(12):1731-1745
12244233
Proc Natl Acad Sci U S A. 2008 Oct 7;105(40):15629-34
18809915
Proc Natl Acad Sci U S A. 2001 Apr 10;98(8):4782-7
11287670
Science. 2003 Aug 1;301(5633):653-7
12893945
Plant Cell. 2006 Jul;18(7):1642-51
16731585
Plant Physiol. 2009 May;150(1):494-505
19329562
Plant Physiol. 2000 Feb;122(2):403-14
10677433
Int Rev Cytol. 2004;234:1-46
15066372
Proc Biol Sci. 2009 Oct 22;276(1673):3561-9
19605392
New Phytol. 2006;171(3):501-23
16866955
Plant Cell. 2008 Jul;20(7):1805-17
18660431
Proc Natl Acad Sci U S A. 2001 Sep 11;98(19):10763-8
11526204
Trends Plant Sci. 2005 Sep;10(9):407-9
16081312
Proc Natl Acad Sci U S A. 2002 Jul 9;99(14):9090-2
12093928
Trends Plant Sci. 2009 Oct;14(10):535-41
19748302
Plant Cell. 2005 Dec;17(12):3337-49
16284305
Plant Cell. 2003 May;15(5):1095-110
12724536
Plant Physiol. 2004 Apr;134(4):1598-613
15047896
Anal Biochem. 1999 Mar 15;268(2):412-3
10075836
J Exp Bot. 2000 Jul;51(348):1211-20
10937696
Plant Cell. 2005 Dec;17(12):3350-61
16284306
J Exp Bot. 2009;60(13):3645-54
19628572
Plant Cell. 2004 May;16(5):1220-34
15105442
Plant Cell Physiol. 1994 Apr;35(3):509-13
8055176
Nucleic Acids Res. 1991 Mar 25;19(6):1349
2030957
New Phytol. 2008;179(1):33-54
18422904
Plant Cell. 2006 Aug;18(8):1887-99
16844907
Trends Plant Sci. 2004 Oct;9(10):490-8
15465684
J Exp Bot. 2003 Jan;54(380):157-68
12456766
Plant Physiol. 2004 May;135(1):432-43
15122038
Development. 2004 Jan;131(2):251-61
14701679
Trends Biochem Sci. 1996 Feb;21(2):70-3
8851664