title: High embryonic recovery rates with in vivo and ex vivo techniques in the bitch.
abstract: The embryonic collection techniques in dogs present a vast methodological variation and low recovery rates. The objectives were to compare and describe two techniques as to the recovery of canine embryos, on the 12th day after the first mating or artificial insemination. Embryos were recovered through uterine horn flushing in vivo, before performing the ovariohysterectomy (OHE) (Group 1; n = 9) or ex vivo, immediately after the OHE (Group 2; n = 9). In total, 43 and 47 embryonic structures were recovered in Groups 1 and 2, respectively. There was no significant difference (p > 0.05) between groups on recovery rates (72.8% and 81.0%, respectively). We inferred that both in vivo and ex vivo techniques allow a high rate of embryonic recovery; in the collection technique prior to the OHE, it is essential to carefully handle the reproductive system during the trans-surgical period and that the 12th day (D12) after the first mating/artificial insemination is an efficient option for the high recovery rate of morulae and blastocysts.
authors.affiliation: Laboratory of Animal Reproduction, Federal University of Espírito Santo (UFES), Alegre, Espírito Santo, Brazil. marceloluz@cca.ufes.br
authors.forename: M R
authors.initials: MR
authors.lastname: Luz
authors.forename: C C
authors.initials: CC
authors.lastname: de Holanda
authors.forename: J J
authors.initials: JJ
authors.lastname: Pereira
authors.forename: P M C
authors.initials: PM
authors.lastname: Freitas
authors.forename: A E P
authors.initials: AE
authors.lastname: Salgado
authors.forename: J Di Giorgio
authors.initials: JD
authors.lastname: Giannotti
authors.forename: S B
authors.initials: SB
authors.lastname: de Oliveira
authors.forename: N S
authors.initials: NS
authors.lastname: Teixeira
authors.forename: C R de Freitas
authors.initials: CR
authors.lastname: Guaitolini
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000502:physiology*; D004285:Dogs / Q000196:embryology*; D004624:Embryo Transfer / Q000662:veterinary*; D005260:Female; D007044:Hysterectomy; D009028:Morula / Q000502:physiology; D010052:Ovariectomy; D011247:Pregnancy