[Preparation and verification of antibodies for five rice receptor-like kinases].
Receptor-like kinase involves self-incompatibility, male sterility, stress responses, and disease resistance. To better understand the physiological function and biological characteristics of rice receptor-like kinase, we cloned five predicted epitope fragments of rice receptor-like kinase. The purified fusion protein was used as antigen to immunize rabbit to get specific polyclonal antibodies. Western blotting analysis shows that the five receptor-like kinases were expressed in rice leaves.
Wang
Bo
B
College of Life Sciences, Agricultural University of Hebei, Baoding 071001, China.
Li
Liyun
L
Cao
Yinghao
Y
Liu
Ziguang
Z
Liu
Guozhen
G
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology; D003001:Cloning, Molecular; D000939:Epitopes / Q000276:immunology; D007114:Immunization; D012275:Oryza / Q000201:enzymology* / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics / Q000276:immunology*; D046549:Plantibodies / Q000276:immunology* / Q000378:metabolism; D011494:Protein Kinases / Q000235:genetics / Q000276:immunology*; D011817:Rabbits
D000911:Antibodies, Monoclonal; D000939:Epitopes; D010940:Plant Proteins; D046549:Plantibodies; D011494:Protein Kinases