Determination of the major constituents in fruit of Arctium lappa L. by matrix solid-phase dispersion extraction coupled with HPLC separation and fluorescence detection.
The arctiin and arctigenin in the fruit of Arctium lappa L. were extracted by matrix solid-phase dispersion (MSPD) and determined by high-performance liquid chromatography (HPLC) with fluorescence detection. The experimental conditions for the MSPD were optimized. Silica gel was selected as dispersion adsorbent and methanol as elution solvent. The calibration curve showed good relationship (r>0.9998) in the concentration range of 0.010-5.0μgmL(-1) for arctiin and 0.025-7.5μgmL(-1) for arctigenin. The recoveries were between 74.4% and 100%. The proposed method consumed less sample, time and solvent compared with conventional methods, including ultrasonic and Soxhlet extraction.
Liu
He
H
College of Chemistry, Jilin University, Changchun 130012, PR China.
Zhang
Yupu
Y
Sun
Yantao
Y
Wang
Xue
X
Zhai
Yujuan
Y
Sun
Ye
Y
Sun
Shuo
S
Yu
Aimin
A
Zhang
Hanqi
H
Wang
Yinghua
Y
D000327:Adsorption; D031170:Arctium / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry*; D005663:Furans / Q000032:analysis* / Q000737:chemistry; D005960:Glucosides / Q000032:analysis* / Q000737:chemistry; D017705:Lignans / Q000032:analysis* / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction / Q000379:methods*; D013050:Spectrometry, Fluorescence
D004365:Drugs, Chinese Herbal; D005663:Furans; D005960:Glucosides; D017705:Lignans; C077992:arctiin; C071942:arctigenin