High-performance liquid chromatography analysis of by-products and intermediates arising during the synthesis of the acetylcholinesterase reactivator HI-6.
An high-performance liquid chromatography (HPLC) method for identification of quaternary and non-quaternary compounds (parent compounds, intermediates, by-products, and products) within the synthesis of the acetylcholinesterase reactivator HI-6, the most promising antidote of nerve agent poisonings, is described. This HPLC method could be of high interest as a quick purity control for those who are interested in development of new acetylcholinesterase reactivators as well as for those who are interested in the synthesis of HI-6 in laboratory or in large-scale production. An HPLC method for quaternary compounds without using common ion-pairing reagents was developed, too.
Jun
Daniel
D
Center of Advanced Studies, Faculty of Military Health Sciences, University of Defence, Hradec Kralove, Czech Republic.
Stodulka
Petr
P
Kuca
Kamil
K
Dolezal
Bohuslav
B
D002384:Catalysis; D002801:Cholinesterase Reactivators / Q000138:chemical synthesis / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D010091:Oximes / Q000138:chemical synthesis / Q000737:chemistry; D011726:Pyridinium Compounds / Q000138:chemical synthesis / Q000737:chemistry
D002801:Cholinesterase Reactivators; D010091:Oximes; D011726:Pyridinium Compounds; C022870:asoxime chloride