Autoinduction of a genetic locus encoding putative acyltransferase in Corynebacterium glutamicum.
A genetic locus, encoding putative acyltransferase, was induced by autoinducers in Corynebacterium glutamicum. The autoinducers were maximally produced by the bacterium after 24 h culture. Those molecules are resistant to proteinase K treatment (300 μg ml(-1)) for 30 min at 37°C or at 121°C for 15 min, and remained stable after extensive storage at 4°C. Autoinducers in the cell-free culture fluids from Corynebacterium ammoniagenes and Pseudomonas aeruginosa also induced the expression of acyltransferase in C. glutamicum, suggesting possible cross-recognition of the autoinducers by C. glutamicum. C. glutamicum thus possesses an autoinduction system which secretes autoinducers during growth, triggering the expression of downstream genes, exemplified by the putative acyltransferase gene.
Shin
Hee-Sung
HS
Department of Biotechnology and Bioinformatics, Korea University, Yeongi, Chungnam, 339-700, Republic of Korea.
Kim
Yong-Jae
YJ
Yoo
In-Hwa
IH
Lee
Heung-Shick
HS
Jin
Shouguang
S
Ha
Un-Hwan
UH
D000217:Acyltransferases / Q000096:biosynthesis* / Q000235:genetics; D003080:Cold Temperature; D048230:Corynebacterium glutamicum / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism*; D019286:Endopeptidase K / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects*; D056426:Genetic Loci; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000378:metabolism
D000217:Acyltransferases; D019286:Endopeptidase K