Diphenyl diselenide in its selenol form has dehydroascorbate reductase and glutathione S-transferase-like activity dependent on the glutathione content.
OBJECTIVE
The antioxidant action of diphenyl diselenide ((PhSe)(2)) is attributed to the mechanism by which (PhSe)(2) has pharmacological activity. Although (PhSe)(2) has glutathione peroxidase mimetic activity, the exact mechanism involved in its antioxidant effect has not yet been completely elucidated. In the present study, mechanisms involved in the antioxidant property of (PhSe)(2) (1-50 microM) were investigated.


METHODS
Dehydroascorbate (DHA) reductase- and glutathione S-transferase (GST)-like activity, 2,2'-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radical-scavenging activity and the protection against the oxidation of Fe(2+) were evaluated.


RESULTS
(PhSe)(2) at concentrations equal to, or greater than, 5 microM showed DHA reductase- and GST-like activity. (PhSe)(2) was not a scavenger of DPPH or ABTS radicals and did not protect against the oxidation of Fe(2+).


CONCLUSIONS
These results clearly indicated that DHA reductase- and GST-like activity are the mechanisms involved in the antioxidant effect of (PhSe)(2).
Luchese
Cristiane
C
Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria, Brazil.
Nogueira
Cristina W
CW
D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D001555:Benzene Derivatives / Q000737:chemistry / Q000494:pharmacology*; D005978:Glutathione / Q000032:analysis; D005982:Glutathione Transferase; D007501:Iron / Q000378:metabolism*; D016566:Organoselenium Compounds / Q000737:chemistry / Q000494:pharmacology*; D010088:Oxidoreductases
D000975:Antioxidants; D001555:Benzene Derivatives; D016566:Organoselenium Compounds; C061132:diphenyldiselenide; D007501:Iron; D010088:Oxidoreductases; C020666:glutathione dehydrogenase (ascorbate); D005982:Glutathione Transferase; D005978:Glutathione