Fluorescence excitation on monolithically integrated all-polymer chips.
All-polymer chips with integrated optical waveguides and microfluidic channels were built as a platform for fluorescence excitation of biological samples. Their functionality has been shown for four cases: (i) fluorescence of labeled phospholipids inside a microfluidic channel, (ii) fluorescence of stained cells inside a microfluidic channel, (iii) fluorescence of labeled phospholipids on top of a polymer waveguide, and (iv) fluorescence of stained cells on top of a polymer waveguide.
Schelb
Mauno
M
Karlsruhe Institute of Technology, Institut fur Mikrostrukturtechnik, Karlsruhe, Germany. mauno.schelb@kit.edu
Vannahme
Christoph
C
Welle
Alexander
A
Lenhert
Steven
S
Ross
Benjamin
B
Mappes
Timo
T
D004867:Equipment Design; D019544:Equipment Failure Analysis; D008029:Lighting / Q000295:instrumentation*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D008856:Microscopy, Fluorescence / Q000295:instrumentation*; D010743:Phospholipids / Q000032:analysis* / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000295:instrumentation*
D010743:Phospholipids