Auxin carriers localization drives auxin accumulation in plant cells infected by Frankia in Casuarina glauca actinorhizal nodules.
Actinorhizal symbioses are mutualistic interactions between plants and the soil bacteria Frankia that lead to the formation of nitrogen-fixing root nodules. Little is known about the signaling mechanisms controlling the different steps of the establishment of the symbiosis. The plant hormone auxin has been suggested to play a role. Here we report that auxin accumulates within Frankia-infected cells in actinorhizal nodules of Casuarina glauca. Using a combination of computational modeling and experimental approaches, we establish that this localized auxin accumulation is driven by the cell-specific expression of auxin transporters and by Frankia auxin biosynthesis in planta. Our results indicate that the plant actively restricts auxin accumulation to Frankia-infected cells during the symbiotic interaction.
Perrine-Walker
Francine
F
UMR DIAPC, Institut de Recherche pour le Développement, Montpellier cedex 5, France.
Doumas
Patrick
P
Lucas
Mikael
M
Vaissayre
Virginie
V
Beauchemin
Nicholas J
NJ
Band
Leah R
LR
Chopard
Jérome
J
Crabos
Amandine
A
Conejero
Geneviève
G
Péret
Benjamin
B
King
John R
JR
Verdeil
Jean-Luc
JL
Hocher
Valérie
V
Franche
Claudine
C
Bennett
Malcolm J
MJ
Tisa
Louis S
LS
Laplaze
Laurent
L
D002352:Carrier Proteins / Q000378:metabolism; D019295:Computational Biology; D040161:Frankia; D020869:Gene Expression Profiling; D007210:Indoleacetic Acids / Q000378:metabolism*; D019684:Magnoliopsida / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology; D008954:Models, Biological; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000378:metabolism; D053204:Root Nodules, Plant / Q000378:metabolism*; D013559:Symbiosis
D002352:Carrier Proteins; D007210:Indoleacetic Acids; D010940:Plant Proteins
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