Limited beneficial effects of perfluorocarbon emulsions on encapsulated cells in culture: experimental and modeling studies.
Due to the high solubility of oxygen in perfluorocarbons (PFCs), these compounds have been explored for improved cell and tissue oxygenation. The goal of this study is to investigate the effects of a PFC emulsion on cellular growth and function in a tissue engineered construct. A perfluorotributylamine (PFTBA) emulsion was co-encapsulated at 10 vol% with mouse βTC-tet insulinoma cells in calcium alginate beads and cultured under normoxic and severely hypoxic conditions. The number of metabolically active cells and the induced insulin secretion rate were measured over time for up to 16 days. Results showed no significant effect of PFTBA relative to the PFTBA-free control. The alginate-PFC-cell system was also modeled mathematically, and simulations tracked the number of viable cells over time under the same conditions used experimentally. Simulations revealed only a small, likely experimentally undetectable difference in cell density between the PFC-containing and PFC-free control beads. It is concluded that PFTBA up to 10 vol% has no significant effect on the growth and function of encapsulated βTC-tet cells under normoxic and hypoxic conditions.
Goh
Fernie
F
School of Chemical & Biomolecular Engineering, Georgia Institute of Technology, 315 Ferst Dr., Atlanta, GA 30332, USA.
Gross
Jeffrey D
JD
Simpson
Nicholas E
NE
Sambanis
Athanassios
A
D000818:Animals; D015687:Cell Hypoxia; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects*; D003198:Computer Simulation; D005466:Fluorocarbons / Q000494:pharmacology*; D007340:Insulinoma; D051379:Mice; D008954:Models, Biological; D010100:Oxygen / Q000378:metabolism*
D005466:Fluorocarbons; C008807:perfluorotributylamine; D010100:Oxygen
Biotechnol Prog. 2008 Mar-Apr;24(2):358-66
18293995
Isr Med Assoc J. 2008 Apr;10(4):273-6
18548980
Cell Transplant. 2000 May-Jun;9(3):415-22
10972340
Transplantation. 2002 Nov 27;74(10):1414-9
12451242
Transplantation. 2006 Nov 27;82(10):1286-90
17130776
Biotechnol Bioeng. 1999;66(4):231-7
10578093
Biotechnol Bioeng. 2007 Sep 1;98(1):261-70
17657780
Biotechnol Bioeng. 2007 Jan 1;96(1):156-66
16917927
Tissue Eng. 2006 Aug;12(8):2077-91
16968150
Cytotechnology. 2006 Jul;51(3):159-70
19002886
Biotechnol Prog. 1995 Mar-Apr;11(2):115-26
7766095
Biochem Biophys Res Commun. 2000 Jul 14;273(3):937-41
10891351
Transplantation. 2007 Oct 15;84(7):864-9
17984839
Cell Transplant. 1997 Jul-Aug;6(4):395-402
9258513
Proc Natl Acad Sci U S A. 1995 Apr 11;92(8):3576-80
7724601
Transplantation. 2002 Dec 27;74(12):1809-11
12499905
Artif Cells Blood Substit Immobil Biotechnol. 2005;33(1):75-82
15768567
Am J Physiol Heart Circ Physiol. 2005 Mar;288(3):H1278-89
15539422
J Theor Biol. 2007 Feb 7;244(3):500-10
17049564
Trends Biotechnol. 1998 Jun;16(6):272-7
9652139
Cell Transplant. 2005;14(7):441-8
16285252
Horm Metab Res. 1991 Jun;23(6):302-3
1916646
Biomaterials. 2003 Dec;24(27):4941-8
14559007
Transplantation. 2004 Jul 15;78(1):96-100
15257045
Transplantation. 2003 May 15;75(9):1524-7
12792508
Biomaterials. 2001 Jun;22(11):1301-10
11336302
Methods Enzymol. 1988;137:575-80
3131633
Transplant Proc. 2005 Oct;37(8):3501-4
16298642
J Comput Assist Tomogr. 1985 Nov-Dec;9(6):1012-9
4056130