title: Local antibiotic delivery with bovine cancellous chips.
abstract: Infected bone defects and osteomyelitis are encountered frequently in trauma cases. Currently, the standard of care for osteomyelitis cases is prolonged systemic antibiotic therapy and implantation of antibiotic carrier beads. However, this method requires a secondary surgery to remove the beads after the infection has cleared. In the present study a common bone void filler was investigated for its ability to be infused with an antibiotic. This study demonstrates that the xenograft material tested can be loaded with gentamicin and release clinically relevant levels of the drug for at least 14 days in vitro allowing for the inhibition of bacterial growth on the graft. This study also demonstrates that the levels of gentamicin released did not have an adverse effect on primary osteoblast cell proliferation or ability to generate alkaline phosphatase. This bone void filler may represent a viable alternative to current methods of local antibiotic delivery in orthopedic applications.
authors.affiliation: Biotechnology Research Group, RTI Biologics, Inc., 11621 Research Circle, Alachua, FL, USA. clewis@rtix.com
authors.forename: Christine S
authors.initials: CS
authors.lastname: Lewis
authors.forename: Jordan
authors.initials: J
authors.lastname: Katz
authors.forename: Maribel I
authors.initials: MI
authors.lastname: Baker
authors.forename: Peter R
authors.initials: PR
authors.lastname: Supronowicz
authors.forename: Elise
authors.initials: E
authors.lastname: Gill
authors.forename: Ronald R
authors.initials: RR
authors.lastname: Cobb
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000493:pharmacokinetics; D001672:Biocompatible Materials; D016025:Bone Transplantation; D002417:Cattle; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation; D003692:Delayed-Action Preparations; D016503:Drug Delivery Systems; D005839:Gentamicins / Q000008:administration & dosage / Q000493:pharmacokinetics; D066298:In Vitro Techniques; D008422:Materials Testing; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects; D010019:Osteomyelitis / Q000188:drug therapy* / Q000201:enzymology / Q000382:microbiology / Q000473:pathology; D051381:Rats; D013203:Staphylococcal Infections / Q000188:drug therapy; D013211:Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development; D014183:Transplantation, Heterologous
chemical_list: D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D003692:Delayed-Action Preparations; D005839:Gentamicins; D000469:Alkaline Phosphatase