[Detection of bovine, goat, pig and chicken derived ingredients in animal products with universal PCR-microarray method].
We analyzed the sequence of vertebrate molecular marker genes, then we selected the mitochondrial DNA (mtDNA) 16S rRNA gene as marker gene. In order to detect four kinds of animal-derived ingredients, which including bovine, goat, pig and chicken. We utilized a pair of universal primers, designed four sets of species-specific microarray probes and two pairs of quality control probes. We optimized the PCR amplifications and hybridization conditions, therefore these four kinds of animal-derived ingredients could be rapid and accurate detected by this approach. The detection limits were all reaches 1 pg. We established the detection platform of these four kinds of animal-derived ingredients. This universal PCR-microarray assay provides a new method for the identification of animal-derived ingredients in the import-export field.
Shi
Fengyun
F
Faculty of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China.
Miao
Jiankun
J
Zhang
Liping
L
Tao
Hong
H
Lü
Jianqiang
J
Ruan
Zhouxi
Z
Zong
Hui
H
D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D002417:Cattle; D002645:Chickens; D004272:DNA, Mitochondrial / Q000235:genetics*; D005506:Food Contamination / Q000032:analysis*; D006041:Goats; D008460:Meat / Q000032:analysis*; D046228:Microarray Analysis / Q000379:methods*; D008931:Mitochondria, Muscle / Q000201:enzymology / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012680:Sensitivity and Specificity; D013552:Swine
D004272:DNA, Mitochondrial; D012336:RNA, Ribosomal, 16S