Alteration of cross-linking selectivity with the 2'-OMe analogue of 2-amino-6-vinylpurine and evaluation of antisense effects.
We previously reported that oligodeoxynucleotides containing 2-amino-6-vinylpurine (2-AVP: 1) exhibit efficient selective cross-linking to cytosine. In this study, the 2'-OMe nucleoside analogue (2) of 2-AVP was designed in order to increase its affinity to RNA and enhance metabolic stability. It has been demonstrated that 2'-OMe oligonucleotides bearing 2 achieve highly selective cross-linking to the thymine base in DNA and show higher antisense effect on luciferase production in cell lysate.
Imoto
Shuhei
S
Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, Aoba-ku, Sendai-shi, Miyagi, Japan.
Hori
Tsuneaki
T
Hagihara
Shinya
S
Taniguchi
Yosuke
Y
Sasaki
Shigeki
S
Nagatsugi
Fumi
F
D000818:Animals; D001483:Base Sequence; D003432:Cross-Linking Reagents / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D049409:Luciferases, Firefly / Q000235:genetics; D016376:Oligonucleotides, Antisense / Q000737:chemistry* / Q000235:genetics; D011687:Purines / Q000737:chemistry*; D012333:RNA, Messenger / Q000235:genetics; D013941:Thymine / Q000737:chemistry*; D014753:Vinyl Compounds / Q000737:chemistry*
C432203:2-amino-6-vinylpurine; D003432:Cross-Linking Reagents; D016376:Oligonucleotides, Antisense; D011687:Purines; D012333:RNA, Messenger; D014753:Vinyl Compounds; D004247:DNA; D049409:Luciferases, Firefly; D013941:Thymine