Urinary matrix metalloproteinase-7 level is associated with the presence of metastasis in bladder cancer.
OBJECTIVE
• To assess the presence of matrix metalloproteinase (MMP)-7 in urine samples of patients with bladder cancer and to investigate the correlation between MMP-7 urine concentration and clinicopathological variables.


METHODS
• The presence of MMP-7 in the urine of patients with bladder cancer was tested in 32 representative cases using immunoprecipitation followed by western blot analysis. • Urinary MMP-7 concentration levels were analyzed in 132 patients with bladder cancer and 96 controls using an enzyme-linked immunosorbent assay.


RESULTS
• MMP-7 levels did not differ significantly between patients with localized bladder cancer and controls (P= 0.174). On the other hand, we detected a fourfold, significantly elevated MMP-7 concentration in urine samples of patients with bladder cancer with regional or distant metastasis (P= 0.003). • Using a threshold value of 6.88 ng/ml, determined by receiver-operating characteristic curve analysis, a specificity of 82% and a sensitivity of 78% were observed. • Western blot analysis revealed that the 55-kDa tissue inhibitor of metalloproteinase 1 complexed MMP-7 is the dominant form of urinary matrilysin.


CONCLUSIONS
• MMP-7 is present in detectable amounts in the urine of patients with bladder cancer. Its concentrations are significantly elevated in patients with metastatic disease. • Determination of urinary matrilysin level could help to detect bladder cancer metastasis, and may therefore provide a more reliable prognosis and influence therapy decisions.
Szarvas
Tibor
T
Department of Urology, University Hospital of Essen, Essen, Germany. sztibusz@gmail.com
Singer
Bernhard B
BB
Becker
Markus
M
Vom Dorp
Frank
F
Jäger
Tobias
T
Szendroi
Attila
A
Riesz
Péter
P
Romics
Imre
I
Rübben
Herbert
H
Ergün
Süleyman
S
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000097:blood / Q000652:urine*; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D020783:Matrix Metalloproteinase 7 / Q000097:blood / Q000652:urine*; D008875:Middle Aged; D011379:Prognosis; D012680:Sensitivity and Specificity; D001749:Urinary Bladder Neoplasms / Q000201:enzymology* / Q000473:pathology* / Q000652:urine
D014408:Biomarkers, Tumor; D020783:Matrix Metalloproteinase 7