Immune detection of acetylcholinesterase in subcellular compartments of Trypanosoma evansi.
Trypanosoma evansi is a worldwide distributed hemoparasite with a strong economic impact in veterinary activities. Despite widespread knowledge about the etiology of the disease caused by T. evansi, there are few detailed studies about the metabolism of this parasite. The aim of this study was to determine the presence of Acetylcholinesterase (AChE) in T. evansi through a strategy of subcellular localization and confocal microscopy. The localization of the AChE by differential and isopycnic centrifugation strategy showed that this enzyme has a predominant localization in the glycosome, similar to hexokinase, and it is not present in either the cytosol or the plasma membrane. This study shows novel data that help to understand the non-neuronal role of AChE in the Trypanosomatidae family.
Mijares
Alfredo
A
Centro de Biofísica y Bioquímica, Laboratorio de Fisiología de Parásitos, Instituto Venezolano de Investigaciones Científicas, A.P. 20632, Caracas 1020A, Venezuela.
Concepción
Juan L
JL
Vielma
Jose R
JR
Portillo
Ramon
R
D000110:Acetylcholinesterase / Q000032:analysis*; D002498:Centrifugation; D006801:Humans; D008830:Microbodies / Q000737:chemistry* / Q000201:enzymology*; D018613:Microscopy, Confocal; D014345:Trypanosoma / Q000737:chemistry* / Q000201:enzymology*
D000110:Acetylcholinesterase
Annu Rev Entomol. 1998;43:1-16
9444749
Arch Biochem Biophys. 1998 Apr 1;352(1):114-20
9521823
Biochem Biophys Res Commun. 2001 Feb 23;281(2):300-4
11181045
Comp Biochem Physiol B Biochem Mol Biol. 1999 Feb;122(2):211-22
10327611
Protein Sci. 1999 Dec;8(12):2553-61
10631970
Exp Parasitol. 1987 Feb;63(1):84-97
3026835
Exp Parasitol. 2010 Jun;125(2):100-5
20093112
FEMS Microbiol Lett. 1992 Aug 15;74(2-3):267-70
1326469
FASEB J. 1992 Feb 1;6(3):845-52
1740234
J Biol Chem. 1951 Nov;193(1):265-75
14907713
Exp Parasitol. 2001 Dec;99(4):213-9
11888248
Parasitol Today. 1996 Feb;12(2):61-5
15275256
Vet J. 1997 Jul;154(1):11-34
9265850
Exp Parasitol. 1970 Dec;28(3):521-34
4993889
J Biol Chem. 1992 Mar 25;267(9):6020-6
1556113
Eur J Biochem. 1999 Sep;264(3):672-86
10491113
Cell Mol Biol (Noisy-le-grand). 1996 Mar;42(2):221-5
8696258
Acta Cient Venez. 1993;44(2):57-66
8085408
Parasitol Today. 1994 Mar;10(3):107-10
15275492
Mol Biochem Parasitol. 2001 Nov;118(1):111-21
11704279
Biochemistry. 1999 Jun 1;38(22):7032-9
10353814
Mol Biochem Parasitol. 2002 Mar;120(1):83-91
11849708
Pharmacol Ther. 1998 Jan;77(1):59-79
9500159
Mol Biol Evol. 1998 May;15(5):518-27
9580980
Trends Biochem Sci. 1990 Jun;15(6):213-6
2200163