Identification of carbohydrate-binding proteins by carbohydrate mimicry peptides.
Peptide-displaying phage technology has numerous applications. Using a specificity-defined monoclonal anticarbohydrate antibody, we can identify a series of short peptides that mimic the binding specificity of a specific carbohydrate. Identified peptides constitute alternatives to the use of carbohydrate ligands in affinity chromatography to isolate a carbohydrate-binding protein. In this chapter, we introduce methods for carbohydrate mimicry peptide affinity chromatographies and discuss their potential use in identifying yet undiscovered carbohydrate-binding proteins in normal and pathological conditions.
Fukuda
Michiko N
MN
Glycobiology Unit, Tumor Microenvironment Program, Cancer Center, Sanford-Burnham Medical Research Institute, La Jolla, California, USA.
Yoneyama
Tohru
T
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism*; D002241:Carbohydrates / Q000737:chemistry*; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism; D051379:Mice; D018716:Molecular Mimicry; D008969:Molecular Sequence Data; D019151:Peptide Library; D011485:Protein Binding
D000911:Antibodies, Monoclonal; D002241:Carbohydrates; D008565:Membrane Proteins; D019151:Peptide Library