title: A rapid microtiter plate assay for measuring the effect of compounds on Staphylococcus aureus membrane potential.
abstract: We developed a homogenous microtiter based assay using the cationic dye 3, 3'-Diethyloxacarbocyanine iodide, DiOC2(3), to measure the effect of compounds on membrane potential in Staphylococcus aureus. In a screen of 372 compounds from a synthetic compound collection with anti-Escherichia coli activity due to unknown modes of action at least 17% demonstrated potent membrane activity, enabling rapid discrimination of nuisance compounds.
authors.affiliation: Antibacterial Discovery Performance Unit, Infectious Diseases Center of Excellence in Drug Discovery, GlaxoSmithKline Pharmaceuticals, Collegeville, PA 19426, United States. dan.r.gentry@gsk.com
authors.forename: Daniel R
authors.initials: DR
authors.lastname: Gentry
authors.forename: Imogen
authors.initials: I
authors.lastname: Wilding
authors.forename: John M
authors.initials: JM
authors.lastname: Johnson
authors.forename: Dongzhao
authors.initials: D
authors.lastname: Chen
authors.forename: Katja
authors.initials: K
authors.lastname: Remlinger
authors.forename: Cindy
authors.initials: C
authors.lastname: Richards
authors.forename: Susan
authors.initials: S
authors.lastname: Neill
authors.forename: Magdalena
authors.initials: M
authors.lastname: Zalacain
authors.forename: Stephen F
authors.initials: SF
authors.lastname: Rittenhouse
authors.forename: Michael N
authors.initials: MN
authors.lastname: Gwynn
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001431:Bacteriological Techniques / Q000379:methods*; D002232:Carbocyanines / Q000378:metabolism; D004926:Escherichia coli / Q000187:drug effects; D057166:High-Throughput Screening Assays / Q000379:methods*; D020128:Inhibitory Concentration 50; D008564:Membrane Potentials / Q000187:drug effects*; D013211:Staphylococcus aureus / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D002232:Carbocyanines; C081554:3,3'-diethyloxacarbocyanine