Molecular characterization of Ramie mosaic virus isolates detected in Jiangsu and Zhejiang provinces, China.
Virus isolates were obtained from three ramie samples (Boehmeria nivea L.) showing yellow mosaic symptoms collected in Jiangsu and Zhejiang provinces, China. Comparison of partial DNA-A fragments amplified with begomovirus universal primers PA/PB revealed that these viral isolates shared a high sequence identity. The complete DNA-A sequences of two isolates J4 and Z1 were determined to be 2736 and 2737 nts, respectively, sharing 94.7% nucleotide sequence identity with each other. Also, the DNA-B components were identified for J4 and Z1 isolates and comprised 2717 and 2719 nts, respectively, sharing 88.6% nucleotide sequence identity with each other. Furthermore, sequence alignment and phylogenetic analysis showed that J4 and Z1 isolates had the highest sequence identities (93.6-94.7%) with isolates of Ramie mosaic virus (RamMV) for DNA-A. These molecular data suggested that J4 and Z1 may be two different isolates of RamMV. This is the first report about the occurrence of a bipartite begomovirus in these regions of China.
Li
J
J
Institute of Biotechnology, Zhejiang University, Hangzhou, P.R. China.
Zhang
X Y
XY
Qian
Y J
YJ
D053839:Begomovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D032402:Boehmeria / Q000821:virology*; D002681:China; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*