title: [Protein kinase D3 is involved in negative regulation of MMP-7 in prostate cancer cells].
abstract: OBJECTIVE
  To explore the role of protein kinase D3 (PKD3) in the regulation of matrix metalloproteinases 7 (MMP-7) expression in prostate cancer cells.
  
  
  METHODS
  PC-3 cells were either stimulated with 100 nmol/L PMA to activate PKD3 kinase activity, or transiently transfected with PKD3 siRNA, and the relative expression level of MMP-7 mRNA were analyzed by real-time PCR using 2(-delta delta Ct) method. MMP-7 mRNA levels were also analyzed and quantified in HEK293 cells with over-expression of wild-type PKD3, PKD3 knockdown (using PKD3 siRNA), or over-expression of wild-type PKD3 followed by PKD3 knockdown.
  
  
  RESULTS
  MMP-7 mRNA expression in PC3 cells was significantly decreased after PMA-induced PKD3 kinase activation. In contrast, PKD3 knockdown by siRNA transfection markedly increased MMP-7 mRNA level (P<0.01). MMP-7 mRNA level in HEK293 cells was significantly decreased by PKD3 over-expression, whereas obviously increased by PKD3 knockdown. Down-regulation of MMP-7 mRNA level in HEK293 induced by PKD3 over-expression was rescued by PKD3 knockdown.
  
  
  CONCLUSIONS
  PKD3 may contribute to the malignant progression of prostate cancer cells through negative regulation of MMP-7 expression.
authors.affiliation: Department of Cell Biology, Southern Medical University, Guangzhou 510515, China. zzp@fimmu.com
authors.forename: Zhi-peng
authors.initials: ZP
authors.lastname: Zou
authors.forename: Li
authors.initials: L
authors.lastname: Feng
authors.forename: Wan-fu
authors.initials: WF
authors.lastname: Xu
authors.forename: Zhi-yong
authors.initials: ZY
authors.lastname: Ke
authors.forename: Q Jane
authors.initials: QJ
authors.lastname: Wang
authors.forename: Fan
authors.initials: F
authors.lastname: Deng
mesh_terms: D045744:Cell Line, Tumor; D015536:Down-Regulation; D055785:Gene Knockdown Techniques; D006801:Humans; D008297:Male; D020783:Matrix Metalloproteinase 7 / Q000378:metabolism*; D011471:Prostatic Neoplasms / Q000201:enzymology / Q000378:metabolism*; D011493:Protein Kinase C / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: C119190:protein kinase C nu; D011493:Protein Kinase C; C060932:MMP7 protein, human; D020783:Matrix Metalloproteinase 7