title: Comparison of tuberculin activity using the interferon-gamma assay for the diagnosis of bovine tuberculosis.
abstract: In this study, interferon-gamma (IFN-gamma) responses in whole blood cultures stimulated with tuberculins from different sources were compared with regard to their diagnostic reliability in cattle experimentally and naturally infected with Mycobacterium bovis. The IFN-gamma responses to different concentrations of purified protein derivatives (PPDs) from M bovis and Mycobacterium avium were quantified. Significant differences (P<0.05) between sources and concentrations of PPDs used for stimulation were detected, indicating a need for standardisation of PPDs used in the IFN-gamma assay. Additionally, a tool named'relative potency 30' that allows rapid comparison of batches and sources of PPDs was defined.
authors.affiliation: Prionics, Wagistrasse 27a, 8952 Schlieren-Zurich, Switzerland.
authors.forename: I
authors.initials: I
authors.lastname: Schiller
authors.forename: H M
authors.initials: HM
authors.lastname: Vordermeier
authors.forename: W R
authors.initials: WR
authors.lastname: Waters
authors.forename: A
authors.initials: A
authors.lastname: Kyburz
authors.forename: M
authors.initials: M
authors.lastname: Cagiola
authors.forename: A
authors.initials: A
authors.lastname: Whelan
authors.forename: M V
authors.initials: MV
authors.lastname: Palmer
authors.forename: T C
authors.initials: TC
authors.lastname: Thacker
authors.forename: J
authors.initials: J
authors.lastname: Meijlis
authors.forename: C
authors.initials: C
authors.lastname: Carter
authors.forename: S
authors.initials: S
authors.lastname: Gordon
authors.forename: T
authors.initials: T
authors.lastname: Egnuni
authors.forename: R
authors.initials: R
authors.lastname: Hardegger
authors.forename: B
authors.initials: B
authors.lastname: Marg-Haufe
authors.forename: A
authors.initials: A
authors.lastname: Raeber
authors.forename: B
authors.initials: B
authors.lastname: Oesch
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood; D002417:Cattle; D046508:Culture Techniques / Q000662:veterinary; D007202:Indicators and Reagents; D007371:Interferon-gamma / Q000096:biosynthesis / Q000097:blood*; D008297:Male; D009162:Mycobacterium avium / Q000276:immunology; D009163:Mycobacterium bovis / Q000276:immunology; D012680:Sensitivity and Specificity; D014373:Tuberculin; D014380:Tuberculosis, Bovine / Q000097:blood / Q000175:diagnosis*
chemical_list: D015415:Biomarkers; D007202:Indicators and Reagents; D014373:Tuberculin; D007371:Interferon-gamma