Genipin-crosslinked silk fibroin/hydroxybutyl chitosan nanofibrous scaffolds for tissue-engineering application.
To improve water-resistant ability and mechanical properties of silk fibroin (SF)/hydroxybutyl chitosan (HBC) nanofibrous scaffolds for tissue-engineering applications, genipin, glutaraldehyde (GTA), and ethanol were used to crosslink electrospun nanofibers, respectively. The mechanical properties of nanofibrous scaffolds were obviously improved after 24 h of crosslinking with genipin and were superior to those crosslinked with GTA and ethanol for 24 h. SEM indicated that crosslinked nanofibers with genipin and GTA vapor had good water-resistant ability. Characterization of the microstructure (porosity and pore structure) demonstrated crosslinked nanofibrous scaffolds with genipin and GTA vapor had lager porosities and mean diameters than those with ethanol. Characterization of FTIR-ATR and (13)C NMR clarified both genipin and GTA acted as crosslinking agents for SF and HBC. Furthermore, genipin could induce SF conformation from random coil or α-helix to β-sheet. Although GTA could also successfully crosslink SF/HBC nanofibrous scaffolds, in long run, genipin maybe a better method due to lower cytotoxicity than GTA. Cell viability studies and wound-healing test in rats clarified that the genipin-crosslinked SF/HBC nanofibrous scaffolds had a good biocompatibility both in vitro and in vivo. These results suggested that genipin-crosslinked SF/HBC nanofibrous scaffolds might be potential candidates for wound dressing and tissue-engineering scaffolds.
Zhang
Kuihua
K
Key Laboratory of Textile Science and Technology Ministry of Education, Donghua University, Shanghai 201620, People's Republic of China.
Qian
Yongfang
Y
Wang
Hongsheng
H
Fan
Linpeng
L
Huang
Chen
C
Yin
Anlin
A
Mo
Xiumei
X
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry*; D002756:Cholagogues and Choleretics / Q000737:chemistry; D003432:Cross-Linking Reagents / Q000737:chemistry; D042783:Endothelial Cells / Q000166:cytology / Q000502:physiology; D005349:Fibroins / Q000737:chemistry*; D057889:Iridoid Glycosides / Q000737:chemistry*; D039823:Iridoids; D008422:Materials Testing; D015394:Molecular Structure; D057139:Nanofibers / Q000737:chemistry*; D016062:Porosity; D011487:Protein Conformation; D051381:Rats; D013552:Swine; D013718:Tensile Strength; D023822:Tissue Engineering / Q000295:instrumentation / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*; D014945:Wound Healing
D001672:Biocompatible Materials; D002756:Cholagogues and Choleretics; D003432:Cross-Linking Reagents; D057889:Iridoid Glycosides; D039823:Iridoids; D005349:Fibroins; D048271:Chitosan; C007834:genipin