Evidence to suggest glutamic acid involvement in Bisphenol A effect at the hypothalamic level in prepubertal male rats.
OBJECTIVE
The aim of present paper was to study the probable role of glutamic acid (GLU) as a mediator of bisphenol A (BPA) effect at the hypothalamic level and its effects on the reproductive axis of prepubertal male rats.


METHODS
Mated Wistar rats were treated with either 0.1% ethanol (control group, n=10) or BPA (BPA group, n=10) in their drinking water until their offspring were weaned at the age of 21 days. The estimated average dose of exposure to dams was approximately 2.5 mg/kg body weight/day of BPA. At the prepubertal stage (35 days of age), the male rats were sacrificed and Gn-RH and glutamic acid (GLU) release, an amino acid involved in Gn-RH secretion, were measured in hypothalamic samples containing medio basal and anterior preoptic area (MBH-APOA), by RIA and HPLC respectively. LH, FSH serum levels were measured by RIA and testosterone by EQLIA.


RESULTS
Gn-RH and GLU release decreased significantly in animals exposed to BPA (p<0.001, p<0.01). LH, FSH and testosterone serum levels were also decreased by treatment (p<0.0001).


CONCLUSIONS
Present results provide evidence that BPA may act at the hypothalamic level to decrease GLU release which in turn may modify Gn-RH secretion altering the normal function of the axis.
Cardoso
Nancy
N
Laboratorio de Endocrinología, Departamento de Fisiología, Facultad de Medicina Universidad de Buenos Aires, Buenos Aires, Argentina.
Pandolfi
Matías
M
Ponzo
Osvaldo
O
Carbone
Silvia
S
Szwarcfarb
Berta
B
Scacchi
Pablo
P
Reynoso
Roxana
R
D000818:Animals; D001559:Benzhydryl Compounds; D002851:Chromatography, High Pressure Liquid; D005260:Female; D005640:Follicle Stimulating Hormone / Q000097:blood; D018698:Glutamic Acid / Q000378:metabolism*; D007987:Gonadotropin-Releasing Hormone / Q000378:metabolism; D007031:Hypothalamus / Q000187:drug effects* / Q000378:metabolism; D007986:Luteinizing Hormone / Q000097:blood; D008297:Male; D010636:Phenols / Q000494:pharmacology*; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects / Q000378:metabolism*; D011863:Radioimmunoassay; D051381:Rats; D017208:Rats, Wistar; D012741:Sexual Maturation / Q000187:drug effects*; D013739:Testosterone / Q000097:blood; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D005640:Follicle Stimulating Hormone; D018698:Glutamic Acid; D007987:Gonadotropin-Releasing Hormone; D007986:Luteinizing Hormone; D010636:Phenols; D013739:Testosterone; C006780:bisphenol A; D000098826:Bisphenol A Compounds