title: Detection of reaginic antibodies against Faenia rectivirgula from the serum of horses affected with Recurrent Airway Obstruction by an in vitro bioassay.
abstract: Reaginic antibodies, mainly of the IgE and some IgG subclasses, play an important role in the induction of type I immediate hypersensitivity reactions. These antibodies bind through their Fc fragment to high affinity receptors (FcεRI) present in the membrane of mast cells and basophils. Previously, several studies have investigated the role of reaginic antibodies in the pathogenesis of RAO. However, whereas immunological aspects of RAO have been extensively studied, the precise sequence of events is still not well understood and role of IgE in this disease still remains controversial. Therefore, in this study a bioassay was developed for reaginic antibody determination in serum from RAO-affected horses in order to determine the etiology of disease. The technique involves measuring in vitro calcium mobilization in RBL-2H3 cells following incubation with horse serum from RAO-affected or unaffected horses and one of the RAO antigens (Faenia rectivirgula). Results demonstrated that 15% of samples from the RAO-affected horses reacted positively in this in vitro bioassay, whereas the samples from unaffected horses did not. This bioassay indicates that reaginic antibodies could be involved in the immunological mechanism leading to RAO; and this technique may facilitate future research in other allergic diseases in horses.
authors.affiliation: Department of Pharmacology, Faculty of Veterinary Science, Universidad Austral de Chile, Valdivia, Chile. gmoran@uach.cl
authors.forename: Gabriel
authors.initials: G
authors.lastname: Morán
authors.forename: Hugo
authors.initials: H
authors.lastname: Folch
authors.forename: Oscar
authors.initials: O
authors.lastname: Araya
authors.forename: Rafael
authors.initials: R
authors.lastname: Burgos
authors.forename: Miguel
authors.initials: M
authors.lastname: Barria
mesh_terms: D039903:Actinobacteria / Q000276:immunology*; D000402:Airway Obstruction / Q000097:blood / Q000276:immunology / Q000662:veterinary*; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D001681:Biological Assay / Q000379:methods / Q000662:veterinary*; D045744:Cell Line, Tumor; D006734:Horse Diseases / Q000097:blood / Q000175:diagnosis / Q000276:immunology*; D006736:Horses; D006967:Hypersensitivity / Q000097:blood / Q000276:immunology / Q000662:veterinary*; D011935:Reagins / Q000032:analysis / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D011935:Reagins
references.citation: Clin Immunol. 2001 May;99(2):198-210
references.pmid: 11318592
references.citation: Equine Vet J. 2001 Nov;33(7):714-20
references.pmid: 11770995
references.citation: Acta Vet Scand. 1988;29(3-4):311-4
references.pmid: 3256230
references.citation: Vet Res Commun. 2010 Jan;34(1):91-9
references.pmid: 20012188
references.citation: Zentralbl Veterinarmed B. 1990 Feb;37(1):64-72
references.pmid: 2346072
references.citation: Equine Vet J. 1993 Jul;25(4):273-5
references.pmid: 8354210
references.citation: J Biol Chem. 1985 Mar 25;260(6):3440-50
references.pmid: 3838314
references.citation: Vet Immunol Immunopathol. 2003 Mar 20;92(1-2):45-60
references.pmid: 12628763
references.citation: Genet Sel Evol. 2003;35 Suppl 1:S177-90
references.pmid: 12927090
references.citation: Chem Immunol. 1995;61:72-87
references.pmid: 7662147
references.citation: J Immunol. 1999 Sep 1;163(5):2508-16
references.pmid: 10452987
references.citation: Vet Immunol Immunopathol. 1993 Oct;38(3-4):201-15
references.pmid: 8291200
references.citation: Vet Immunol Immunopathol. 1998 Dec 11;66(3-4):245-56
references.pmid: 9880102
references.citation: J Allergy. 1966 Mar;37(3):169-85
references.pmid: 4160257
references.citation: Vet Immunol Immunopathol. 2009 Nov 15;132(1):21-30
references.pmid: 19819562
references.citation: Equine Vet J. 1993 Jul;25(4):261-7
references.pmid: 8354208
references.citation: Equine Vet J. 1979 Jul;11(3):159-66
references.pmid: 385306
references.citation: Equine Vet J. 2001 Jan;33(1):5-19
references.pmid: 11191611
references.citation: Chem Immunol. 1994;59:173-90
references.pmid: 7945926
references.citation: Equine Vet J. 2000 Sep;32(5):393-400
references.pmid: 11037260
references.citation: Vet Immunol Immunopathol. 2006 Sep 15;113(1-2):99-112
references.pmid: 16797724
references.citation: Am J Vet Res. 1988 Jun;49(6):933-8
references.pmid: 3400931
references.citation: Biochem J. 1994 Dec 1;304 ( Pt 2):431-40
references.pmid: 7998977
references.citation: J Immunol. 1996 Jun 15;156(12):4876-83
references.pmid: 8648137
references.citation: J Appl Physiol (1985). 1985 Feb;58(2):598-604
references.pmid: 3980362
references.citation: Immunol Rev. 1992 Feb;125:37-48
references.pmid: 1532373
references.citation: J Vet Med A Physiol Pathol Clin Med. 2007 Feb;54(1):40-7
references.pmid: 17359454
references.citation: J Biol Chem. 2001 May 4;276(18):14814-20
references.pmid: 11278575
references.citation: Immunochemistry. 1977 Feb;14(2):81-4
references.pmid: 15944
references.citation: J Biol Chem. 1995 Feb 24;270(8):4013-22
references.pmid: 7876149
references.citation: Vet Immunol Immunopathol. 2008 Dec 15;126(3-4):351-61
references.pmid: 18775570
references.citation: Vet Immunol Immunopathol. 2000 Mar 15;73(3-4):241-53
references.pmid: 10713338
references.citation: J Biol Chem. 1997 Dec 12;272(50):31225-9
references.pmid: 9395446
references.citation: Vet Immunol Immunopathol. 2004 Oct;101(3-4):243-50
references.pmid: 15350754
references.citation: Vet Immunol Immunopathol. 2009 Jan 15;127(1-2):85-93
references.pmid: 19027178
references.citation: J Vet Intern Med. 2004 Jan-Feb;18(1):92-7
references.pmid: 14765737