title: Testing susceptibility of multidrug-resistant Mycobacterium tuberculosis to second-line drugs by use of blood agar.
abstract: In this study, the susceptibilities of 35 multidrug-resistant (MDR) Mycobacterium tuberculosis clinical isolates to second-line drugs, including kanamycin (KM), rifabutin (RBU), ofloxacin (OFX), p-aminosalicylic acid (PAS), capreomycin (CAP), clofazimine (CFM), and ethionamide (ETH), were investigated on blood agar according to CLSI recommendations. Compared with the results of the Bactec 460 TB system, agreement was 100, 100, 97, 100, 100, 100, and 86% for KM, RBU, OFX, PAS, CAP, CFM, and ETH, respectively. Compared with the results of the proportion method, agreement was 100, 100, 97, 100, 97, 100, and 77% for KM, RBU, OFX, PAS, CAP, CFM, and ETH, respectively.
authors.affiliation: Istanbul University, Istanbul Medical School, Department of Microbiology and Clinical Microbiology, Istanbul, Turkey.
authors.forename: Dilek
authors.initials: D
authors.lastname: Satana
authors.forename: Ahmet Yilmaz
authors.initials: AY
authors.lastname: Coban
authors.forename: Meltem
authors.initials: M
authors.lastname: Uzun
mesh_terms: D000362:Agar; D000995:Antitubercular Agents / Q000494:pharmacology*; D001769:Blood; D003470:Culture Media / Q000737:chemistry*; D024901:Drug Resistance, Multiple, Bacterial; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000302:isolation & purification; D018088:Tuberculosis, Multidrug-Resistant / Q000382:microbiology*
chemical_list: D000995:Antitubercular Agents; D003470:Culture Media; D000362:Agar
references.citation: Dan Med Bull. 2009 Nov;56(4):208-10
references.pmid: 19939338
references.citation: J Clin Microbiol. 2003 Apr;41(4):1710-1
references.pmid: 12682165
references.citation: J Clin Microbiol. 1999 Oct;37(10):3179-86
references.pmid: 10488174
references.citation: PLoS Negl Trop Dis. 2007 Nov 28;1(2):e83
references.pmid: 18060087
references.citation: J Clin Microbiol. 2009 Nov;47(11):3630-4
references.pmid: 19741086
references.citation: J Clin Microbiol. 1981 May;13(5):908-12
references.pmid: 6787076
references.citation: Annu Rev Med. 2009;60:307-20
references.pmid: 19630575
references.citation: J Chemother. 2008 Jun;20(3):388-90
references.pmid: 18606599
references.citation: Saudi Med J. 2007 Jun;28(6):975-7
references.pmid: 17530127
references.citation: J Antimicrob Chemother. 2004 Jul;54(1):130-3
references.pmid: 15190018
references.citation: J Clin Microbiol. 2008 Dec;46(12):4095-8
references.pmid: 18945843
references.citation: J Chemother. 2004 Aug;16(4):334-6
references.pmid: 15332706
references.citation: J Clin Microbiol. 2005 Apr;43(4):1930-1
references.pmid: 15815022
references.citation: Int J Tuberc Lung Dis. 2006 Apr;10(4):450-3
references.pmid: 16602412