Regeneration and transdetermination: the role of wingless and its regulation.
Imaginal discs of Drosophila have the remarkable ability to regenerate. After fragmentation wound healing occurs, ectopic wg is induced and a blastema is formed. In some, but not all fragments, the blastema will replace missing structures and a few cells can become more plastic and transdetermine to structures of other discs. A series of systematic cuts through the first leg disc revealed that a cut must transect the dorsal-proximal disc area and that the fragment must also include wg-competent cells. Fragments that fail to both transdetermine and regenerate missing structures will do both when provided with exogenous Wg, demonstrating the necessity of Wg in regenerative processes. In intact leg discs ubiquitously expressed low levels of Wg also leads to blastema formation, regeneration and transdetermination. Two days after exogenous wg induction the endogenous gene is activated, leading to elevated levels of Wg in the dorsal aspect of the leg disc. We identified a wg enhancer that regulates ectopic wg expression. Deletion of this enhancer increases transdetermination, but lowers the amount of ectopic Wg. We speculate that this lessens repression of dpp dorsally, and thus creates a permissive condition under which the balance of ectopic Wg and Dpp is favorable for transdetermination.
Schubiger
Margrit
M
Department of Biology, Box 351800, University of Washington, Seattle, WA 98105, USA. margrit@u.washington.edu
Sustar
Anne
A
Schubiger
Gerold
G
D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D029721:Drosophila Proteins / Q000235:genetics* / Q000502:physiology*; D004331:Drosophila melanogaster / Q000235:genetics* / Q000254:growth & development / Q000502:physiology*; D004742:Enhancer Elements, Genetic; D005121:Extremities / Q000254:growth & development / Q000502:physiology; D017344:Genes, Insect; D012038:Regeneration / Q000235:genetics* / Q000502:physiology*; D015533:Transcriptional Activation; D051155:Wnt1 Protein / Q000235:genetics* / Q000502:physiology*
D017931:DNA Primers; D029721:Drosophila Proteins; D051155:Wnt1 Protein; C064196:dpp protein, Drosophila; C074622:wg protein, Drosophila
Development. 1995 Oct;121(10):3359-69
7588069
Wilhelm Roux Arch Entwickl Mech Org. 1968 Mar;160(1):41-75
28304493
Dev Cell. 2009 Mar;16(3):358-73
19289082
EMBO J. 1993 Dec 15;12(13):5293-302
8262072
Wilhelm Roux Arch Entwickl Mech Org. 1968 Sep;161(3):249-280
28304471
Nature. 2005 Nov 10;438(7065):234-7
16281037
Proc Natl Acad Sci U S A. 2008 Jul 15;105(28):9715-20
18621688
Dev Dyn. 2003 Feb;226(2):202-10
12557199
Development. 1998 Aug;125(15):2771-80
9655800
Nature. 2009 Jul 2;460(7251):60-5
19571878
J Biol. 2004;3(3):11
15186480
Wilhelm Roux Arch Entwickl Mech Org. 1933 Sep;130(3-4):747-770
28354371
Science. 1996 Sep 6;273(5280):1373-7
8703069
Development. 2005 Aug;132(16):3753-65
16077094
Dev Biol. 1976 Feb;48(2):461-5
815114
Science. 2008 Jan 18;319(5861):327-30
18063755
Development. 2009 Apr;136(7):1169-77
19244279
Dev Biol. 2008 Jul 1;319(1):68-77
18485344
Cold Spring Harb Symp Quant Biol. 2008;73:583-92
19028989
Dev Cell. 2009 Jun;16(6):797-809
19531351
Nature. 1994 Dec 1;372(6505):461-4
7984239
PLoS Biol. 2010 Jun 08;8(6):e1000390
20543996
Science. 2005 Dec 23;310(5756):1919-23
16373567
Dev Biol. 1970 Jul;22(3):389-411
5423309
Dev Biol. 1971 Oct;26(2):277-95
5003476
Development. 2007 Feb;134(3):479-89
17185322
Wilhelm Roux Arch Entwickl Mech Org. 1970 Mar;166(1):1-44
28304536
Bioessays. 1995 Apr;17(4):299-309
7741723
Development. 1995 May;121(5):1263-72
7789260
Mech Dev. 2000 Jul;95(1-2):89-99
10906453
Dev Biol. 1993 May;157(1):240-50
8482414
Dev Biol. 1988 May;127(1):197-208
2452103
Proc Natl Acad Sci U S A. 1975 Nov;72(11):4674-8
812100
Development. 1996 Nov;122(11):3519-29
8951067
Nature. 1994 Mar 17;368(6468):208-14
8145818
Wilhelm Roux Arch Entwickl Mech Org. 1968 Mar;160(1):9-40
28304492
Cell. 2005 Feb 11;120(3):383-93
15707896
Dev Biol. 1983 May;97(1):212-21
6404675
Experientia. 1973 May 15;29(5):631-2
4199754
J Embryol Exp Morphol. 1974 Jun;31(3):557-72
4448938
Genes Dev. 2006 Dec 1;20(23):3232-7
17114576
J Exp Zool. 1975 Jul;193(1):49-77
806653
PLoS One. 2010 Jan 07;5(1):e8510
20062803
Wilhelm Roux Arch Entwickl Mech Org. 1935 Jan;133(1-2):156-192
28354978
Dev Biol. 1981 Oct 15;87(1):64-75
7286422
J Embryol Exp Morphol. 1966 Feb;15(1):77-111
5915174
J Exp Zool. 1981 Apr;216(1):45-61
6793689
Science. 2008 Jan 18;319(5861):323-7
18063757
Development. 2010 Apr;137(7):1169-79
20215351
Dev Biol. 2005 Apr 1;280(1):73-86
15766749
Proc Natl Acad Sci U S A. 2009 Mar 17;106(11):4290-5
19237582
Development. 1999 Apr;126(8):1591-9
10079222
Cell. 1993 Feb 26;72(4):527-40
8440019
Wilehm Roux Arch Dev Biol. 1977 Jun;183(2):85-100
28304897
Int J Dev Biol. 2005;49(4):391-9
15968584