Comparison of tuberculin activity using the interferon-gamma assay for the diagnosis of bovine tuberculosis.
In this study, interferon-gamma (IFN-gamma) responses in whole blood cultures stimulated with tuberculins from different sources were compared with regard to their diagnostic reliability in cattle experimentally and naturally infected with Mycobacterium bovis. The IFN-gamma responses to different concentrations of purified protein derivatives (PPDs) from M bovis and Mycobacterium avium were quantified. Significant differences (P<0.05) between sources and concentrations of PPDs used for stimulation were detected, indicating a need for standardisation of PPDs used in the IFN-gamma assay. Additionally, a tool named'relative potency 30' that allows rapid comparison of batches and sources of PPDs was defined.
Schiller
I
I
Prionics, Wagistrasse 27a, 8952 Schlieren-Zurich, Switzerland.
Vordermeier
H M
HM
Waters
W R
WR
Kyburz
A
A
Cagiola
M
M
Whelan
A
A
Palmer
M V
MV
Thacker
T C
TC
Meijlis
J
J
Carter
C
C
Gordon
S
S
Egnuni
T
T
Hardegger
R
R
Marg-Haufe
B
B
Raeber
A
A
Oesch
B
B
D000818:Animals; D015415:Biomarkers / Q000097:blood; D002417:Cattle; D046508:Culture Techniques / Q000662:veterinary; D007202:Indicators and Reagents; D007371:Interferon-gamma / Q000096:biosynthesis / Q000097:blood*; D008297:Male; D009162:Mycobacterium avium / Q000276:immunology; D009163:Mycobacterium bovis / Q000276:immunology; D012680:Sensitivity and Specificity; D014373:Tuberculin; D014380:Tuberculosis, Bovine / Q000097:blood / Q000175:diagnosis*
D015415:Biomarkers; D007202:Indicators and Reagents; D014373:Tuberculin; D007371:Interferon-gamma