title: Pancreatic beta cell mass PET imaging and quantification with [11C]DTBZ and [18F]FP-(+)-DTBZ in rodent models of diabetes.
abstract: OBJECTIVE
  The aim of this study is to compare the utility of two positron emission tomography (PET) imaging ligands ((+)-[(11)C]dihydrotetrabenazine ([(11)C]DTBZ) and the fluoropropyl analog ([(18)F]FP-(+)-DTBZ)) that target islet β-cell vesicular monoamine transporter type II to measure pancreatic β-cell mass (BCM).
  
  
  METHODS
  [(11)C]DTBZ or [(18)F]FP-(+)-DTBZ was injected, and serial PET images were acquired in rat models of diabetes (streptozotocin-treated and Zucker diabetic fatty) and β-cell compensation (Zucker fatty). Radiotracer standardized uptake values (SUV) were correlated to pancreas insulin content measured biochemically and histomorphometrically.
  
  
  RESULTS
  On a group level, a positive correlation of [(11)C]DTBZ pancreatic SUV with pancreas insulin content and BCM was observed. In the STZ diabetic model, both [(18)F]FP-(+)-DTBZ and [(11)C]DTBZ correlated positively with BCM, although only ∼25% of uptake could be attributed to β-cell uptake. [(18)F]FP-(+)-DTBZ displacement studies indicate that there is a substantial fraction of specific binding that is not to pancreatic islet β cells.
  
  
  CONCLUSIONS
  PET imaging with [(18)F]FP-(+)-DTBZ provides a noninvasive means to quantify insulin-positive BCM and may prove valuable as a diagnostic tool in assessing treatments to maintain or restore BCM.
authors.affiliation: Department of Diagnostic Radiology, Yale University School of Medicine, New Haven, CT, USA.
authors.forename: Tarun
authors.initials: T
authors.lastname: Singhal
authors.forename: Yu-Shin
authors.initials: YS
authors.lastname: Ding
authors.forename: David
authors.initials: D
authors.lastname: Weinzimmer
authors.forename: Marc D
authors.initials: MD
authors.lastname: Normandin
authors.forename: David
authors.initials: D
authors.lastname: Labaree
authors.forename: Jim
authors.initials: J
authors.lastname: Ropchan
authors.forename: Nabeel
authors.initials: N
authors.lastname: Nabulsi
authors.forename: Shu-fei
authors.initials: SF
authors.lastname: Lin
authors.forename: Marc B
authors.initials: MB
authors.lastname: Skaddan
authors.forename: Walter C
authors.initials: WC
authors.lastname: Soeller
authors.forename: Yiyun
authors.initials: Y
authors.lastname: Huang
authors.forename: Richard E
authors.initials: RE
authors.lastname: Carson
authors.forename: Judith L
authors.initials: JL
authors.lastname: Treadway
authors.forename: Gary W
authors.initials: GW
authors.lastname: Cline
mesh_terms: D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000000981:diagnostic imaging* / Q000473:pathology; D007328:Insulin / Q000378:metabolism; D007515:Islets of Langerhans / Q000000981:diagnostic imaging* / Q000378:metabolism / Q000473:pathology; D049268:Positron-Emission Tomography; D051381:Rats; D011924:Rats, Zucker; D013311:Streptozocin
chemical_list: D007328:Insulin; D013311:Streptozocin
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