[Construction of recombinant adenovirus containing TK gene and its effect against human liver cancer cells].
OBJECTIVE
To construct a replication-defective adenovirus containing TK gene and investigate the killing effects of TK gene against human liver cancer cells SMMC-7721.


METHODS
The recombinant adenovirus ADV-TK was constructed using homologous recombination in the cells. SMMC-7721 cells transfected with recombined adenovirus were exposed to GCV, and the cell viability was measured by MTT assays.


RESULTS
The recombinant adenovirus containing TK gene was successfully constructed. Transfection by the recombinant adenovirus ADV-TK and GCV exposure significantly suppressed the growth of SMMC-7721 cells.


CONCLUSIONS
A replication-defective adenovirus containing TK gene has been successfully constructed, and in combination with GCV, the recombinant adenovirus produces significant killing effect against SMMC-7721 cells in vitro.
Chen
Geng-zhen
GZ
Third Department of Surgery, Second Affiliated Hospital, Shantou University Medical College, Shantou 515041, China. chengengzhen@tom.com
Hu
Hui
H
Xu
Ming-yan
MY
Deng
Xiao-ling
XL
D000256:Adenoviridae / Q000235:genetics*; D045744:Cell Line, Tumor; D015316:Genetic Therapy; D005822:Genetic Vectors; D006801:Humans; D008113:Liver Neoplasms / Q000628:therapy*; D013937:Thymidine Kinase / Q000235:genetics
D013937:Thymidine Kinase