Immunization with plant-expressed hemagglutinin protects chickens from lethal highly pathogenic avian influenza virus H5N1 challenge infection.
Highly pathogenic avian influenza (HPAI) is a striking disease in susceptible poultry, which leads to severe economic losses. Inactivated vaccines are the most widely used vaccines in avian influenza virus (AIV) vaccination programs. However, these vaccines interfere with the serological detection of wild-type AIV infections in immunized populations. The use of vaccines that allow differentiation between infected and vaccinated animals (DIVA strategy) would stop current stamping-out policies. Therefore, novel vaccination strategies are needed to allow improved protection of animals and humans against HPAI virus (HPAIV) infection. The presented study analyzed for the first time the immunogenic capacity of plant-expressed full-length hemagglutinin (rHA0) of HPAIV H5N1 in several vaccine formulations within the highly relevant host species chicken. We were able to express plant-expressed rHA0 at high levels and could show that, when administered with potent adjuvants, it is highly immunogenic and can fully protect chicken against lethal challenge infection. Real-time reverse transcription (RT)-PCR and serological tests demonstrated only marginally increased virus replication in animals vaccinated with plant-derived rHA0 compared to animals immunized with an inactivated reference vaccine. In addition, the use of plant-expressed rHA0 also allowed an easy serological differentiation of vaccinated from AIV-infected animals based on antibodies against the influenza virus NP protein.
Kalthoff
Donata
D
Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Südufer 10, 17493 Greifswald-Insel Riems, Germany.
Giritch
Anatoli
A
Geisler
Katharina
K
Bettmann
Ulrike
U
Klimyuk
Victor
V
Hehnen
Hans-Robert
HR
Gleba
Yuri
Y
Beer
Martin
M
D000818:Animals; D002645:Chickens; D015870:Gene Expression; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000008:administration & dosage / Q000235:genetics* / Q000276:immunology*; D007114:Immunization; D053124:Influenza A Virus, H5N1 Subtype / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity / Q000502:physiology; D007252:Influenza Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D005585:Influenza in Birds / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D014026:Nicotiana / Q000235:genetics* / Q000378:metabolism
D019267:Hemagglutinin Glycoproteins, Influenza Virus; D007252:Influenza Vaccines; D011994:Recombinant Proteins
Vaccine. 2009 May 26;27(25-26):3467-70
19200814
Rev Sci Tech. 2009 Apr;28(1):319-32
19618635
Ann Oncol. 2010 Dec;21(12):2420-2427
20494963
Biosci Rep. 2007 Dec;27(6):359-72
17597393
Comp Immunol Microbiol Infect Dis. 2009 Jul;32(4):351-63
18442853
Nat Biotechnol. 2005 Jun;23(6):718-23
15883585
Plant Biotechnol J. 2008 Dec;6(9):930-40
19076615
J Gen Virol. 2007 Feb;88(Pt 2):554-558
17251574
J Vet Med Sci. 2008 Nov;70(11):1147-52
19057130
Vaccine. 1999 May 4;17(18):2265-74
10403594
Nature. 1970 Aug 15;227(5259):680-5
5432063
FEBS Lett. 1994 Aug 15;350(1):5-8
7545946
Proc Natl Acad Sci U S A. 2006 May 23;103(21):8197-202
16717197
PLoS One. 2010 May 14;5(5):e10645
20498717
Anal Biochem. 1993 Jul;212(1):154-9
8368489
Vaccine. 2006 Mar 15;24(12):2176-85
16310896
Avian Pathol. 2004 Aug;33(4):393-404
15370036
Proc Natl Acad Sci U S A. 2004 May 4;101(18):6852-7
15103020
Glycoconj J. 1995 Feb;12(1):84-93
7540902
Vaccine. 2005 Apr 22;23(22):2943-52
15780743
Plant Biotechnol J. 2007 Sep;5(5):570-8
17561926
Vaccine. 2009 Feb 11;27(7):1087-92
19100806
J Clin Microbiol. 2007 Feb;45(2):600-3
17182758
J Biol Stand. 1977;5(3):237-47
408355
Proc Natl Acad Sci U S A. 2006 Oct 3;103(40):14701-6
16973752
Avian Dis. 2004 Sep;48(3):663-8
15529992
Vaccine. 1988 Dec;6(6):504-8
2854339
Rev Sci Tech. 2000 Aug;19(2):463-82
10935274
Vaccine. 2009 Jan 29;27(5):773-85
19041677
Avian Dis. 2009 Sep;53(3):376-81
19848075