Evaluation of tumour necrosis factor-alpha and interleukin-1beta in an experimental pyelonephritis model induced with planktonic and biofilms cells of Pseudomonas aeruginosa.
Urinary tract infections may induce severe inflammation, transient impairment in renal function and scar formation, ranging in severity from acute symptomatic pyelonephritis to chronic pyelonephritis, and have the potential to lead to renal failure and death. In the present study, the relationship between production of tumour necrosis factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta), neutrophil recruitment, bacterial colonization and tissue damage was investigated using a mouse model of acute ascending pyelonephritis induced with planktonic and biofilm cells of Pseudomonas aeruginosa. Neutrophil influx correlated with rise in TNF-alpha and IL-1beta, indicating an association between these cytokines and neutrophil infiltration. However, biofilm cells of P aeruginosa induced higher levels of TNF-alpha and IL-1beta leading to higher neutrophil infiltration causing tissue damage, assessed in terms of malondialdehyde, lactate dehydrogenase and glutathione content, which may have contributed to bacterial persistence compared with their planktonic counterparts. The results of the present investigation suggest that exaggerated cytokine production during P aeruginosa-induced pyelonephritis causes tissue damage operative through neutrophil recruitment leading to bacterial persistence in host tissues. The findings of the present study may be relevant for the better understanding of disease pathophysiology and for the future developments of preventive strategies against pyelonephritis based on anti-inflammatory intervention.
Mittal
Rahul
R
Division of Infectious Diseases, Childrens Hospital Los Angeles, Los Angeles, California, USA;
Sharma
Saroj
S
Chhibber
Sanjay
S
Harjai
Kusum
K
Curr Opin Pharmacol. 2007 Jun;7(3):244-51
17418640
Microbes Infect. 2000 Jul;2(9):1051-60
10967285
Infect Immun. 2005 Jun;73(6):3693-701
15908399
J Infect Dis. 1982 Aug;146(2):220-6
7108272
Arch Dis Child. 1994 Feb;70(2):111-5
8129430
J Immunol. 2000 Jun 15;164(12):6576-82
10843717
Trends Microbiol. 2001 Jan;9(1):34-9
11166241
Biochem J. 1966 Jun;99(3):667-76
5964963
J Appl Microbiol. 2002;92 Suppl:163S-70S
12000625
Clin Microbiol Rev. 1997 Oct;10(4):742-80
9336671
Annu Rev Microbiol. 1995;49:711-45
8561477
Am J Respir Crit Care Med. 1995 Dec;152(6 Pt 1):2111-8
8520783
Nature. 2003 Nov 20;426(6964):306-10
14628055
Comp Immunol Microbiol Infect Dis. 2006 Jan;29(1):12-26
16427697
Annu Rev Microbiol. 2002;56:187-209
12142477
Curr Opin Microbiol. 2008 Dec;11(6):560-6
18935979
Pediatr Nephrol. 2005 Nov;20(11):1599-604
16001281
Curr Opin Pediatr. 2006 Apr;18(2):148-52
16601494
Cell Microbiol. 2009 Jul;11(7):1034-43
19374653
Microbes Infect. 2005 Jan;7(1):132-7
15716072
Nat Rev Microbiol. 2007 Jan;5(1):48-56
17143318
Appl Environ Microbiol. 1999 Aug;65(8):3710-3
10427070
Kidney Int. 2004 Oct;66(4):1334-44
15458426
Clin Microbiol Infect. 2008 Apr;14(4):337-43
18190582
Nature. 2001 Jul 26;412(6845):442-5
11473319
Clin Diagn Lab Immunol. 2001 Nov;8(6):1060-3
11687440
J Med Microbiol. 2007 Nov;56(Pt 11):1549-1557
17965358
J Urol. 1987 Dec;138(6):1451-6
2890772
J Am Soc Nephrol. 2007 Nov;18(11):2810-6
17942949
Curr Opin Microbiol. 2006 Feb;9(1):33-9
16406777
J Bacteriol. 1994 Apr;176(8):2137-42
8157581
Microbes Infect. 2001 Jan;3(1):23-35
11226851
Indian J Med Res. 2006 Jan;123(1):67-72
16567871
Pediatr Nephrol. 2005 Oct;20(10):1445-8
16079986
Infect Immun. 1989 Jul;57(7):2196-202
2543635
Emerg Infect Dis. 2002 Sep;8(9):881-90
12194761
J Biomed Sci. 2008 Nov;15(6):731-41
18688758
Eur Arch Otorhinolaryngol. 1995;252 Suppl 1:S59-60
7537567
PLoS Pathog. 2009 Mar;5(3):e1000354
19325879
Cornea. 2008 Apr;27(3):320-6
18362661
J Antimicrob Chemother. 1992 Apr;29 Suppl A:19-24
1601752
Future Microbiol. 2008 Feb;3(1):97-106
18230038
Nephrology (Carlton). 2007 Oct;12(5):487-93
17803473
Eur J Pharmacol. 2007 Feb 14;557(1):69-75
17173892
Curr Opin Microbiol. 2009 Feb;12(1):61-6
19168385
Am J Respir Crit Care Med. 2005 Jun 1;171(11):1209-23
15695491
Drugs. 2007;67(3):351-68
17335295
Chemotherapy. 1998 May-Jun;44(3):190-7
9612609
Crit Care Med. 2005 Dec;33(12 Suppl):S460-2
16340421
Kidney Int. 2008 Mar;73(6):787-8
18309351
Trends Microbiol. 2005 Jan;13(1):7-10
15639625
Microbes Infect. 2004 Nov;6(14):1326-32
15555540
Microb Pathog. 2008 Nov-Dec;45(5-6):323-30
18771720
Respir Care. 2009 May;54(5):628-40
19393107
Science. 1999 May 21;284(5418):1318-22
10334980
Rev Infect Dis. 1983 Mar-Apr;5(2):279-313
6405475
Clin Microbiol Rev. 2002 Apr;15(2):167-93
11932229
Pediatr Clin North Am. 1997 Oct;44(5):1133-69
9326956
Infect Immun. 1990 Jul;58(7):2383-5
2114367