title: Patterning of peptide nucleic acids using reactive microcontact printing.
abstract: PNAs (peptide nucleic acids) have been immobilized onto surfaces in a fast, accurate way by employing reactive microcontact printing. Surfaces have been first modified with aldehyde groups to react with the amino end of the synthesized PNAs. When patterning fluorescein-labeled PNAs by reactive microcontact printing using oxygen-oxidized polydimethylsiloxane stamps, homogeneous arrays were fabricated and characterized using optical methods. PNA-patterned surfaces were hybridized with complementary and mismatched dye-labeled oligonucleotides to test their ability to recognize DNA sequences. The stability and selectivity of the PNA-DNA duplexes on surfaces have been verified by fluorescence microscopy, and the melting curves have been recorded. Finally, the technique has been applied to the fabrication of chips by spotting a PNA microarray onto a flat PDMS stamp and reproducing the same features onto many slides. The chips were finally applied to single nucleotide polymorphism detection on oligonucleotides.
authors.affiliation: Molecular Nanofabrication and Biophysical Engineering, Department of Science and Technology, University of Twente, PEnschede, The Netherlands.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Calabretta
authors.forename: Dorothee
authors.initials: D
authors.lastname: Wasserberg
authors.forename: Geertruida A
authors.initials: GA
authors.lastname: Posthuma-Trumpie
authors.forename: Vinod
authors.initials: V
authors.lastname: Subramaniam
authors.forename: Aart
authors.initials: A
authors.lastname: van Amerongen
authors.forename: Roberto
authors.initials: R
authors.lastname: Corradini
authors.forename: Tullia
authors.initials: T
authors.lastname: Tedeschi
authors.forename: Stefano
authors.initials: S
authors.lastname: Sforza
authors.forename: David N
authors.initials: DN
authors.lastname: Reinhoudt
authors.forename: Rosangela
authors.initials: R
authors.lastname: Marchelli
authors.forename: Jurriaan
authors.initials: J
authors.lastname: Huskens
authors.forename: Pascal
authors.initials: P
authors.lastname: Jonkheijm
mesh_terms: D008856:Microscopy, Fluorescence; D020135:Peptide Nucleic Acids / Q000737:chemistry*
chemical_list: D020135:Peptide Nucleic Acids