The Lrs4-Csm1 monopolin complex associates with kinetochores during anaphase and is required for accurate chromosome segregation.
Lrs4 and Csm1, components of the monopolin complex, localize to the rDNA where they regulate rDNA maintenance and segregation. During meiosis, the complex also associates with kinetochores to bring about sister kinetochore co-orientation, an essential aspect of meiosis I chromosome segregation. We show here that the Lrs4-Csm1 complex associates with kinetochores during mitosis. This kinetochore localization is observed during anaphase and depends on the on the Mitotic Exit Network, a signaling cascade essential for the completion of mitosis. Furthermore, we find that Lrs4 and Csm1 are important for chromosome segregation fidelity. Our results reveal a previously unanticipated function for Lrs4-Csm1 in mitotic chromosome segregation.
Brito
Ilana L
IL
David H. Koch Institute for Integrative Cancer Research and Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA, USA.
Monje-Casas
Fernando
F
Amon
Angelika
A
D000705:Anaphase / Q000235:genetics*; D018797:Cell Cycle Proteins / Q000032:analysis* / Q000378:metabolism; D020090:Chromosome Segregation; D018386:Kinetochores / Q000378:metabolism*; D008938:Mitosis; D009687:Nuclear Proteins / Q000032:analysis* / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000032:analysis* / Q000378:metabolism; D015398:Signal Transduction
C474335:CSM1 protein, S cerevisiae; D018797:Cell Cycle Proteins; C474336:LRS4 protein, S cerevisiae; C112472:NDC80 protein, S cerevisiae; D009687:Nuclear Proteins; D029701:Saccharomyces cerevisiae Proteins
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