title: Scanning electron microscopy (SEM) and X-ray dispersive spectrometry evaluation of direct laser metal sintering surface and human bone interface: a case series.
abstract: Recent studies have shown that direct laser metal sintering (DLMS) produces structures with complex geometry and consequently that allow better osteoconductive properties. The aim of this patient report was to evaluate the early bone response to DLMS implant surface retrieved from human jaws. Four experimental DLMS implants were inserted in the posterior mandible of four patients during conventional dental implant surgery. After 8 weeks, the micro-implants and the surrounding tissue were removed and prepared for scanning electron microscopy (SEM) and histomorphometric analysis to evaluate the bone-implant interface. The SEM and EDX evaluations showed a newly formed tissue composed of calcium and phosphorus. The bone-to-implant contact presented a mean of 60.5 ± 11.6%. Within the limits of this patient report, data suggest that the DLMS surfaces presented a close contact with the human bone after a healing period of 8 weeks.
authors.affiliation: Department of Biomaterials, University of Insubria-Varese, Varese, Italy.
authors.forename: Carlo
authors.initials: C
authors.lastname: Mangano
authors.forename: Adriano
authors.initials: A
authors.lastname: Piattelli
authors.forename: Mario
authors.initials: M
authors.lastname: Raspanti
authors.forename: Francesco
authors.initials: F
authors.lastname: Mangano
authors.forename: Alessandra
authors.initials: A
authors.lastname: Cassoni
authors.forename: Giovanna
authors.initials: G
authors.lastname: Iezzi
authors.forename: Jamil Awad
authors.initials: JA
authors.lastname: Shibli
mesh_terms: D000328:Adult; D000497:Alloys; D015921:Dental Implants; D005260:Female; D006801:Humans; D053844:Lasers, Solid-State; D008297:Male; D008334:Mandible / Q000473:pathology / Q000601:surgery; D008855:Microscopy, Electron, Scanning; D008875:Middle Aged; D016348:Osseointegration; D013052:Spectrometry, X-Ray Emission; D013499:Surface Properties; D013997:Time Factors; D014025:Titanium
chemical_list: D000497:Alloys; D015921:Dental Implants; C031462:titanium alloy (TiAl6V4); D014025:Titanium
references.citation: Biomaterials. 1996 Jan;17(1):31-5
references.pmid: 8962945
references.citation: Clin Oral Implants Res. 2001 Aug;12(4):350-7
references.pmid: 11488864
references.citation: J Cell Mol Med. 2004 Apr-Jun;8(2):169-80
references.pmid: 15256065
references.citation: J Mater Sci Mater Med. 2007 Jul;18(7):1263-77
references.pmid: 17443395
references.citation: J Periodontol. 2006 Oct;77(10):1736-43
references.pmid: 17032118
references.citation: J Biomed Mater Res B Appl Biomater. 2009 May;89(2):325-334
references.pmid: 18837456
references.citation: Int J Prosthodont. 1998 Sep-Oct;11(5):391-401
references.pmid: 9922731
references.citation: J Biomed Mater Res B Appl Biomater. 2005 Jul;74(1):636-42
references.pmid: 15803487
references.citation: Biomaterials. 2001 Oct;22(19):2671-82
references.pmid: 11519787
references.citation: Dent Mater. 2008 Nov;24(11):1525-33
references.pmid: 18502498
references.citation: FASEB J. 1990 Oct;4(13):3111-23
references.pmid: 2210157
references.citation: Int J Oral Maxillofac Implants. 2003 May-Jun;18(3):341-8
references.pmid: 12814308
references.citation: Clin Implant Dent Relat Res. 2010 Dec;12(4):281-8
references.pmid: 19438956
references.citation: J Biomed Mater Res A. 2009 Mar 1;88(3):823-31
references.pmid: 18481810
references.citation: Clin Oral Implants Res. 2001 Apr;12(2):128-34
references.pmid: 11251662
references.citation: J Biomed Mater Res. 1999 May;45(2):75-83
references.pmid: 10397960
references.citation: Adv Dent Res. 1999 Jun;13:38-48
references.pmid: 11276745
references.citation: J Biomed Mater Res A. 2008 Jun 1;85(3):664-73
references.pmid: 17876801
references.citation: J Biomed Mater Res B Appl Biomater. 2007 Feb;80(2):377-85
references.pmid: 16850464
references.citation: J Biomed Mater Res. 1995 Mar;29(3):389-401
references.pmid: 7542245
references.citation: Biomaterials. 1997 Apr;18(7):577-9
references.pmid: 9105598