title: The Bombyx mori nucleopolyhedrovirus (BmNPV) ODV-E56 envelope protein is also a per os infectivity factor.
abstract: The Bombyx mori nucleopolyhedrovirus (BmNPV) odv-e56 gene is a late gene and encodes an occlusion-derived virus (ODV)-specific envelope protein, ODV-E56. To determine its role in the BmNPV life cycle, an odv-e56 null virus, BmE56D, was constructed through homologous recombination. A repaired virus was also constructed, named BmE56DR. The production of budded virion (BV) and polyhedra, the replication of viral DNA, and the morphological of infected BmN cells were analyzed, revealing no significant difference among the BmE56D, the wild-type (WT), and the BmE56DR virus. Larval bioassays demonstrated that injection of BmE56D BV into the hemocoel could kill B. mori larvae as efficiently as repaired and WT viruses, however BmE56D was unable to infect the B. mori larvae when inoculated per os. Thus, these results indicated that ODV-E56 envelope protein of BmNPV is also a per os infectivity factor (PIF), but is not essential for virus replication.
authors.affiliation: College of Animal Sciences, Zhejiang University, Huajiachi Campus, Hangzhou 310029, PR China.
authors.forename: Xingwei
authors.initials: X
authors.lastname: Xiang
authors.forename: Lin
authors.initials: L
authors.lastname: Chen
authors.forename: Aiqin
authors.initials: A
authors.lastname: Guo
authors.forename: Shaofang
authors.initials: S
authors.lastname: Yu
authors.forename: Rui
authors.initials: R
authors.lastname: Yang
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Wu
mesh_terms: D000818:Animals; D012831:Bombyx / Q000821:virology*; D005816:Genetic Complementation Test; D007814:Larva / Q000821:virology; D017924:Nucleopolyhedroviruses / Q000235:genetics / Q000472:pathogenicity*; D011995:Recombination, Genetic; D016019:Survival Analysis; D014759:Viral Envelope Proteins / Q000172:deficiency / Q000378:metabolism*; D014774:Virulence; D037521:Virulence Factors / Q000172:deficiency / Q000378:metabolism*; D014779:Virus Replication
chemical_list: D014759:Viral Envelope Proteins; D037521:Virulence Factors