title: Characterization of nucleosides and nucleobases in fruits of Ziziphus jujuba by UPLC-DAD-MS.
abstract: The fruit of Ziziphus jujuba , named dazao in Chinese, has been utilized as food as well as crude drugs in China for thousands of years. To explore the profiles of the nucleosides and nucleobases in this fruit, an ultraperformance liquid chromatograph coupled with a photodiode array detector and electrospray ionization-mass spectrometer method (UPLC-DAD-MS) has been established and validated in this paper. The validated method was successfully applied for the simultaneous characterization and quantitation of 9 nucleosides and nucleobases in 49 dazao samples, which comprised 43 cultivars from 26 cultivation regions. Furthermore, principal component analysis (PCA) was performed to classify the samples on the basis of the contents of the nine analyzed compounds. The results showed that almost all of these dazao samples were rich in nucleosides and nucleobases, although their contents were obviously various, and the proposed method could serve as a prerequisite for quality control of jujube products.
authors.affiliation: Jiangsu Key Laboratory for TCM Formulae Research, Nanjing University of Chinese Medicine, Nanjing 210046, People's Republic of China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Guo
authors.forename: Jin-Ao
authors.initials: JA
authors.lastname: Duan
authors.forename: Yu-Ping
authors.initials: YP
authors.lastname: Tang
authors.forename: Zhen-Hua
authors.initials: ZH
authors.lastname: Zhu
authors.forename: Ye-Fei
authors.initials: YF
authors.lastname: Qian
authors.forename: Nian-Yun
authors.initials: NY
authors.lastname: Yang
authors.forename: Er-Xin
authors.initials: EX
authors.lastname: Shang
authors.forename: Da-Wei
authors.initials: DW
authors.lastname: Qian
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D000242:Cyclic AMP / Q000032:analysis; D006152:Cyclic GMP / Q000032:analysis; D003890:Desiccation; D005638:Fruit / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods*; D009705:Nucleosides / Q000032:analysis*; D009711:Nucleotides / Q000032:analysis*; D010936:Plant Extracts / Q000737:chemistry; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry; D031957:Ziziphus / Q000737:chemistry*
chemical_list: D009705:Nucleosides; D009711:Nucleotides; D010936:Plant Extracts; D000242:Cyclic AMP; D006152:Cyclic GMP