title: Optical coherence tomography allows for the reliable identification of laryngeal epithelial dysplasia and for precise biopsy: a clinicopathological study of 61 patients undergoing microlaryngoscopy.
abstract: OBJECTIVE
  A newly developed microscope-based spectral-domain optical coherence tomography (SD-OCT) device and an endoscope-based time-domain OCT (TD-OCT) were used to assess the inter-rater reliability, sensitivity, specificity, and accuracy of benign and dysplastic laryngeal epithelial lesions.
  
  
  METHODS
  Prospective study.
  
  
  METHODS
  OCT during microlaryngoscopy was done on 35 patients with an endoscope-based TD-OCT, and on 26 patients by an SD-OCT system integrated into an operating microscope. Biopsies were taken from microscopically suspicious lesions allowing comparative study of OCT images and histology.
  
  
  RESULTS
  Thickness of the epithelium was seen to be the main criterion for degree of dysplasia. The inter-rater reliability for two observers was found to be kappa = 0.74 (P <.001) for OCT. OCT provided test outcomes for differentiation between benign laryngeal lesions and dysplasia/CIS with sensitivity of 88%, specificity of 89%, PPV of 85%, NPV of 91%, and predictive accuracy of 88%. However, because of the limited penetration depth of the laser light primarily in hyperkeratotic lesions (thickness above 1.5 mm), the basal cell layer was no longer visible, precluding reliable assessment of such lesions.
  
  
  CONCLUSIONS
  OCT allows for a fairly accurate assessment of benign and dysplastic laryngeal epithelial lesion and greatly facilitates the taking of precise biopsies. Laryngoscope, 2010.
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, University of Rostock, Rostock, Germany. tino.just@med.unirostock.de
authors.forename: Tino
authors.initials: T
authors.lastname: Just
authors.forename: Eva
authors.initials: E
authors.lastname: Lankenau
authors.forename: Friedrich
authors.initials: F
authors.lastname: Prall
authors.forename: Gereon
authors.initials: G
authors.lastname: Hüttmann
authors.forename: Hans Wilhelm
authors.initials: HW
authors.lastname: Pau
authors.forename: Konrad
authors.initials: K
authors.lastname: Sommer
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001706:Biopsy; D005260:Female; D006801:Humans; D007818:Laryngeal Diseases / Q000473:pathology*; D007828:Laryngoscopy / Q000379:methods*; D008297:Male; D008875:Middle Aged; D011230:Precancerous Conditions / Q000473:pathology*; D011446:Prospective Studies; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D041623:Tomography, Optical Coherence