Characterization of Bacillus phage-K2 isolated from chungkookjang, a fermented soybean foodstuff.
An investigation of a virulent Bacillus phage-K2 (named Bp-K2) isolated from chungkookjang (a fermented soybean foodstuff) was made. Bp-K2 differed in infectivity against a number of Bacillus subtilis strains including starter strains of chungkookjang and natto, being more infectious to Bacillus strains isolated from the chungkookjang, but much less active against a natto strain. Bp-K2 is a small DNA phage whose genome size is about 21 kb. Bp-K2 is a tailed bacteriophage with an isometric icosahedral head (50 nm long on the lateral side, 80 nm wide), a long contractile sheath (85-90 nm × 28 nm), a thin tail fiber (80-85 nm long, 10 nm wide), and a basal plate (29 nm long, 47 nm wide) with a number of spikes, but no collar. The details of the structures of Bp-K2 differ from natto phage ϕBN100 as well as other known Bacillus phages such as SPO1-like or ϕ 29-like viruses. These data suggest that Bp-K2 would be a new member of the Myoviridae family of Bacillus bacteriophages.
Kim
Eun Ju
EJ
Department of Microbiology, Chungbuk National University, Cheongju, Chungbuk, 361-763, Korea.
Hong
Jeong Won
JW
Yun
Na-Rae
NR
Lee
Young Nam
YN
D017103:Bacillus Phages / Q000145:classification / Q000302:isolation & purification* / Q000648:ultrastructure; D001412:Bacillus subtilis / Q000821:virology; D005285:Fermentation; D005516:Food Microbiology; D046529:Microscopy, Electron, Transmission; D017900:Myoviridae / Q000145:classification / Q000302:isolation & purification* / Q000648:ultrastructure; D013025:Glycine max / Q000821:virology*
Appl Environ Microbiol. 1997 Oct;63(10):4087-9
16535718
Arch Virol. 1994;135(3-4):333-44
7979971
J Virol. 1983 Apr;46(1):239-49
6402605
J Nutr. 2006 May;136(5):1323-8
16614424
Microbiol Mol Biol Rev. 2001 Jun;65(2):261-87 ; second page, table of contents
11381102
J Virol. 1983 Apr;46(1):250-9
6402606
Vet Microbiol. 2008 Dec 10;132(3-4):340-7
18676101
Antonie Van Leeuwenhoek. 2000 Aug;78(2):123-7
11204764
Appl Environ Microbiol. 2008 Jul;74(13):4070-8
18469131
J Nutr Sci Vitaminol (Tokyo). 2005 Aug;51(4):254-9
16261997
Appl Environ Microbiol. 1996 Jul;62(7):2482-8
8779587
Appl Environ Microbiol. 2003 May;69(5):2491-7
12732513