title: Fluorescent labeling of newborn dentate granule cells in GAD67-GFP transgenic mice: a genetic tool for the study of adult neurogenesis.
abstract: Neurogenesis in the adult hippocampus is an important form of structural plasticity in the brain. Here we report a line of BAC transgenic mice (GAD67-GFP mice) that selectively and transitorily express GFP in newborn dentate granule cells of the adult hippocampus. These GFP(+) cells show a high degree of colocalization with BrdU-labeled nuclei one week after BrdU injection and express the newborn neuron marker doublecortin and PSA-NCAM. Compared to mature dentate granule cells, these newborn neurons show immature morphological features: dendritic beading, fewer dendritic branches and spines. These GFP(+) newborn neurons also show immature electrophysiological properties: higher input resistance, more depolarized resting membrane potentials, small and non-typical action potentials. The bright labeling of newborn neurons with GFP makes it possible to visualize the details of dendrites, which reach the outer edge of the molecular layer, and their axon (mossy fiber) terminals, which project to the CA3 region where they form synaptic boutons. GFP expression covers the whole developmental stage of newborn neurons, beginning within the first week of cell division and disappearing as newborn neurons mature, about 4 weeks postmitotic. Thus, the GAD67-GFP transgenic mice provide a useful genetic tool for studying the development and regulation of newborn dentate granule cells.
authors.affiliation: Department of Neurobiology, Duke University Medical Center, Durham, North Carolina, United States of America.
authors.forename: Shengli
authors.initials: S
authors.lastname: Zhao
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhou
authors.forename: Jimmy
authors.initials: J
authors.lastname: Gross
authors.forename: Pei
authors.initials: P
authors.lastname: Miao
authors.forename: Li
authors.initials: L
authors.lastname: Qiu
authors.forename: Dongqing
authors.initials: D
authors.lastname: Wang
authors.forename: Qian
authors.initials: Q
authors.lastname: Chen
authors.forename: Guoping
authors.initials: G
authors.lastname: Feng
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D018891:Dentate Gyrus / Q000737:chemistry* / Q000166:cytology* / Q000254:growth & development / Q000378:metabolism; D005260:Female; D005968:Glutamate Decarboxylase / Q000235:genetics* / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D055495:Neurogenesis; D009474:Neurons / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D013194:Staining and Labeling
chemical_list: D049452:Green Fluorescent Proteins; D005968:Glutamate Decarboxylase; C402565:glutamate decarboxylase 1
references.citation: Cell. 2004 Oct 15;119(2):257-72
references.pmid: 15479642
references.citation: J Comp Neurol. 2003 Dec 1;467(1):60-79
references.pmid: 14574680
references.citation: J Neurosci. 2006 Apr 12;26(15):4095-103
references.pmid: 16611826
references.citation: Neurosci Lett. 1996 Jul 5;212(1):41-4
references.pmid: 8823758
references.citation: Hippocampus. 2006;16(3):312-20
references.pmid: 16435314
references.citation: Trends Mol Med. 2006 Sep;12(9):400-5
references.pmid: 16890023
references.citation: J Comp Neurol. 2007 Nov 10;505(2):190-208
references.pmid: 17853439
references.citation: J Neurosci. 2004 Mar 31;24(13):3251-9
references.pmid: 15056704
references.citation: Proc Natl Acad Sci U S A. 2006 Feb 7;103(6):1912-7
references.pmid: 16446451
references.citation: Proc Natl Acad Sci U S A. 2006 Nov 14;103(46):17501-6
references.pmid: 17088541
references.citation: J Neurosci. 2003 Jul 2;23(13):5594-8
references.pmid: 12843261
references.citation: Nature. 2001 Mar 15;410(6826):372-6
references.pmid: 11268214
references.citation: Nature. 2004 May 13;429(6988):184-7
references.pmid: 15107864
references.citation: Eur J Neurosci. 2006 Sep;24(6):1535-45
references.pmid: 17004917
references.citation: Nature. 1973 Aug 17;244(5416):442-3
references.pmid: 4582499
references.citation: Nat Rev Neurosci. 2006 Mar;7(3):179-93
references.pmid: 16495940
references.citation: J Neurosci. 2000 May 1;20(9):3354-68
references.pmid: 10777798
references.citation: Prog Neurobiol. 2009 Oct;89(2):162-75
references.pmid: 19615423
references.citation: Neuron. 1994 Jan;12(1):11-24
references.pmid: 8292356
references.citation: Mol Cell Neurosci. 2003 Jul;23(3):373-82
references.pmid: 12837622
references.citation: Nat Neurosci. 2002 May;5(5):438-45
references.pmid: 11953752
references.citation: J Comp Neurol. 1965 Jun;124(3):319-35
references.pmid: 5861717
references.citation: Neurosci Biobehav Rev. 2009 Mar;33(3):232-52
references.pmid: 18786562
references.citation: Curr Biol. 2002 Apr 2;12(7):606-8
references.pmid: 11937032
references.citation: Nature. 2008 Feb 21;451(7181):1004-7
references.pmid: 18235445
references.citation: Brain Res Brain Res Rev. 2005 Feb;48(1):43-56
references.pmid: 15708627
references.citation: J Neurophysiol. 2005 Dec;94(6):4528-32
references.pmid: 16033936
references.citation: Curr Opin Pharmacol. 2006 Feb;6(1):114-9
references.pmid: 16359920
references.citation: J Physiol. 2008 Aug 15;586(16):3783-93
references.pmid: 18565997
references.citation: Genome Res. 2002 Dec;12(12):1992-8
references.pmid: 12466304
references.citation: Nature. 2006 Aug 24;442(7105):929-33
references.pmid: 16906136
references.citation: Development. 2003 Jan;130(2):391-9
references.pmid: 12466205
references.citation: Nature. 2006 Feb 2;439(7076):589-93
references.pmid: 16341203
references.citation: J Comp Neurol. 1996 Sep 30;373(4):593-618
references.pmid: 8889946
references.citation: J Comp Neurol. 2004 Jul 12;475(1):128-41
references.pmid: 15176089
references.citation: Hippocampus. 2003;13(3):413-27
references.pmid: 12722981
references.citation: J Comp Neurol. 1975 Jan 15;159(2):149-75
references.pmid: 1112911
references.citation: Methods Mol Med. 2004;99:255-67
references.pmid: 15131343
references.citation: J Neurosci. 1996 Mar 15;16(6):2027-33
references.pmid: 8604047
references.citation: Nat Neurosci. 2010 Feb;13(2):232-8
references.pmid: 20081852
references.citation: Neuroreport. 2000 Jun 26;11(9):1991-6
references.pmid: 10884058
references.citation: Nat Neurosci. 1999 Mar;2(3):260-5
references.pmid: 10195219
references.citation: Neuroscience. 1995 Dec;69(3):705-9
references.pmid: 8596641
references.citation: Nat Neurosci. 2004 Nov;7(11):1233-41
references.pmid: 15494728
references.citation: Annu Rev Neurosci. 2005;28:223-50
references.pmid: 16022595
references.citation: J Comp Neurol. 1991 Jan 8;303(2):177-92
references.pmid: 1672874
references.citation: J Neurosci. 2006 Feb 22;26(8):2326-34
references.pmid: 16495460
references.citation: Proc Natl Acad Sci U S A. 1999 Apr 27;96(9):5263-7
references.pmid: 10220454
references.citation: Curr Opin Neurobiol. 2004 Apr;14(2):186-91
references.pmid: 15082323
references.citation: Prog Neurobiol. 2003 Dec;71(5):337-58
references.pmid: 14757115
references.citation: J Neurosci. 1998 May 1;18(9):3386-403
references.pmid: 9547246
references.citation: Physiol Rev. 2005 Apr;85(2):523-69
references.pmid: 15788705
references.citation: Nat Med. 1998 Nov;4(11):1313-7
references.pmid: 9809557
references.citation: Nature. 2002 Feb 28;415(6875):1030-4
references.pmid: 11875571
references.citation: PLoS One. 2008 Apr 09;3(4):e1959
references.pmid: 18509506
references.citation: Science. 2003 Aug 8;301(5634):805-9
references.pmid: 12907793
references.citation: Neuron. 2004 Mar 4;41(5):683-6
references.pmid: 15003168
references.citation: Rev Neurosci. 2007;18(2):93-114
references.pmid: 17593874
references.citation: Nat Rev Neurosci. 2000 Oct;1(1):67-73
references.pmid: 11252770
references.citation: J Neurosci. 2006 Jan 4;26(1):3-11
references.pmid: 16399667
references.citation: Nature. 2001 May 24;411(6836):480-4
references.pmid: 11373681
references.citation: Neuroscience. 2005;130(4):843-52
references.pmid: 15652983
references.citation: Neurosci Lett. 2003 Dec 15;353(1):69-71
references.pmid: 14642440
references.citation: J Comp Neurol. 1990 Nov 15;301(3):365-81
references.pmid: 2262596
references.citation: Neuron. 2000 Oct;28(1):41-51
references.pmid: 11086982