title: Reinke's edema: investigations on the role of MIB-1 and hepatocyte growth factor.
abstract: Reinke's edema is a benign disease of the human vocal fold, which mainly affects the sub-epithelial layer of the vocal fold. Microscopic observations show a strongly oedematous epithelium with loosened intercellular junctions, a disruption of the extracellular connections between mucosal epithelium and connective tissue, closely adherent to the thyroarytenoid muscle. Thickening of the basal layer of epithelium, known as Reinke's space, high deposition of fibronectin and chronic inflammatory infiltration it is also visible. We analyzed, together with the hepatocyte growth factor (HGF), the expression level of MIB-1 in samples harvested from patients affected by Reinke's edema, in order to define its biological role and consider it as a possible prognostic factor in the follow-up after surgical treatment. We observed a moderate expression of HGF in the lamina propria of the human vocal fold and in the basal membrane of the mucosal epithelium. Our finding suggests that this growth factor acts as an antifibrotic agent in Reinke's space and affects the fibronectin deposition in the lamina propria. MIB-1, on the contrary, showed a weak expression in the basement membrane of the mucosal epithelium and a total absence in the lamina propria deep layer, thus suggesting that only the superficial layer is actively involved in the reparatory process with a high regenerative capacity, together with a high deposition of fibronectin. The latter is necessary for the cellular connections reconstruction, after the inflammatory infiltration.
authors.affiliation: Department of Otorhinolaringology,Audiology and Phoniatry G. Ferreri, University of Rome La Sapienza, piazzale Aldo Moro 5, Rome, Italy. marco.artico@uniroma1.it
authors.forename: M
authors.initials: M
authors.lastname: Artico
authors.forename: E
authors.initials: E
authors.lastname: Bronzetti
authors.forename: B
authors.initials: B
authors.lastname: Ionta
authors.forename: M
authors.initials: M
authors.lastname: Bruno
authors.forename: A
authors.initials: A
authors.lastname: Greco
authors.forename: G
authors.initials: G
authors.lastname: Ruoppolo
authors.forename: A
authors.initials: A
authors.lastname: De Virgilio
authors.forename: L
authors.initials: L
authors.lastname: Longo
authors.forename: M
authors.initials: M
authors.lastname: De Vincentiis
mesh_terms: D002478:Cells, Cultured; D005786:Gene Expression Regulation; D017228:Hepatocyte Growth Factor / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000378:metabolism*; D007819:Laryngeal Edema / Q000378:metabolism* / Q000473:pathology*; D014827:Vocal Cords / Q000378:metabolism* / Q000473:pathology
chemical_list: D019394:Ki-67 Antigen; D017228:Hepatocyte Growth Factor
references.citation: Ann Otol Rhinol Laryngol. 1998 Dec;107(12):1023-8
references.pmid: 9865632
references.citation: HNO. 2007 Jul;55(7):583-98
references.pmid: 17431565
references.citation: Singapore Med J. 2009 Feb;50(2):201-7
references.pmid: 19296037
references.citation: Pol J Pathol. 2003;54(1):61-4
references.pmid: 12817882
references.citation: Laryngoscope. 1999 Oct;109(10):1684-9
references.pmid: 10522943
references.citation: Ann Otol Rhinol Laryngol. 1999 Nov;108(11 Pt 1):1068-72
references.pmid: 10579234
references.citation: Ann Otol Rhinol Laryngol. 1995 Jan;104(1):13-8
references.pmid: 7832537
references.citation: Ann Otol Rhinol Laryngol. 2003 Dec;112(12):1026-33
references.pmid: 14703105
references.citation: Laryngoscope. 2008 Aug;118(8):1500-3
references.pmid: 18528307
references.citation: J Otolaryngol. 2002 Dec;31(6):376-80
references.pmid: 12593551
references.citation: Ann Otol Rhinol Laryngol. 1995 Sep;104(9 Pt 1):698-703
references.pmid: 7661518
references.citation: Acta Otorhinolaryngol Belg. 1996;50(4):253-64
references.pmid: 9001635
references.citation: Mol Med Rep. 2008 May-Jun;1(3):347-53
references.pmid: 21479416
references.citation: Curr Opin Otolaryngol Head Neck Surg. 2005 Jun;13(3):148-51
references.pmid: 15908811
references.citation: Anat Embryol (Berl). 1992 Sep;186(4):341-6
references.pmid: 1416083
references.citation: EXS. 1993;65:131-65
references.pmid: 8380735
references.citation: Acta Otolaryngol. 1999 Mar;119(2):271-6
references.pmid: 10320090
references.citation: Acta Otolaryngol. 2004 May;124(4):505-8
references.pmid: 15224884
references.citation: Laryngoscope. 2003 Dec;113(12):2187-91
references.pmid: 14660925
references.citation: Laryngoscope. 2004 Dec;114(12):2161-7
references.pmid: 15564837
references.citation: Acta Otolaryngol. 1993 Jan;113(1):98-101
references.pmid: 8442430