Quantitation of acute phase proteins and protein electrophoresis in monitoring the acute inflammatory process in experimentally and naturally infected mice.
Serologic screening for infectious disease in sentinel mice from rodent colonies is expensive and labor-intensive, often involving multiple assays for several different infectious agents. Previously, we established normal reference ranges for the protein fractions of several laboratory strains of mice by using a commercially available agarose system of protein electrophoresis. In the current study, we address protein fractionation and quantitation of acute phase proteins (APP) in mice experimentally infected with Sendai virus or mouse parvovirus. We further investigate this methodology by using samples from sentinel mice from colonies with endemic infection. All study groups showed significant increases in gamma globulins. Various other protein fractions showed mild variable changes; significant differences were not detected for individual APP. These results contrast the significant changes observed in APP and protein electrophoresis by using the standard methods of inducing inflammatory responses through injection of complete Freund adjuvant or LPS. These present data suggest that although quantitation of individual APP may not be helpful, gamma globulin levels may reflect infection in laboratory mice and provide a possible adjunct to traditional screening methods.
Cray
Carolyn
C
Division of Comparative Pathology, Department of Pathology, University of Miami Miller School of Medicine, Miami, Florida, USA. c.cray@miami.edu
Besselsen
David G
DG
Hart
Jody L
JL
Yoon
David
D
Rodriguez
Marilyn
M
Zaias
Julia
J
Altman
Norman H
NH
D000209:Acute-Phase Proteins / Q000032:analysis* / Q000378:metabolism; D000704:Analysis of Variance; D000818:Animals; D001797:Blood Protein Electrophoresis / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005620:Freund's Adjuvant; D008070:Lipopolysaccharides; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008991:Monitoring, Physiologic / Q000379:methods*; D010322:Parvoviridae Infections / Q000175:diagnosis / Q000378:metabolism*; D010253:Respirovirus Infections / Q000175:diagnosis / Q000378:metabolism*; D005719:gamma-Globulins / Q000032:analysis
D000209:Acute-Phase Proteins; D008070:Lipopolysaccharides; D005719:gamma-Globulins; D005620:Freund's Adjuvant
ILAR J. 2008;49(3):316-25
18506065
ILAR J. 2008;49(3):272-6
18506060
Parasitol Int. 2001 Mar;50(1):15-26
11267928
J Am Assoc Lab Anim Sci. 2009 Jul;48(4):387-90
19653947
Electrophoresis. 2004 Sep;25(17):3055-65
15349948
Shock. 2006 Jun;25(6):581-5
16721265
Res Vet Sci. 1983 Jul;35(1):58-60
6622845
J Microbiol Immunol Infect. 1999 Jun;32(2):73-82
11561579
J Immunol. 1983 Feb;130(2):885-9
6600258
Vet Clin Pathol. 2002;31(3):140-51
12189602
Nature. 1966 Sep 17;211(5055):1298-300
5969813
N Engl J Med. 1974 Aug 8;291(6):287-90
4276105
Vet Res. 2004 Mar-Apr;35(2):163-87
15099494
Cornell Vet. 1982 Oct;72(4):416-26
7140303
Arch Toxicol. 2003 Jul;77(7):392-402
12669191
Int Immunopharmacol. 2003 May;3(5):765-74
12757745
ILAR J. 2000;41(2):99-104
11406703
Acta Histochem. 2006;108(3):229-32
16714050
J Immunol. 1992 Aug 15;149(4):1319-25
1354233
Parasitol Int. 2000 Feb;48(4):297-301
10725693
J Gen Virol. 2006 Jun;87(Pt 6):1543-1556
16690918
Vet Immunol Immunopathol. 1994 Mar;40(3):225-41
8160361
Exp Lung Res. 2005 Nov-Dec;31(9-10):855-71
16684717
J Immunol Methods. 1992 Aug 30;153(1-2):31-40
1517600
Vet Clin Pathol. 2005 Jun;34(2):85-99
15902658
N Engl J Med. 1999 Feb 11;340(6):448-54
9971870
Vet Rec. 1997 Sep 20;141(12):299-303
9330474
Lab Anim Sci. 1996 Aug;46(4):370-80
8872986
Proteomics. 2005 Nov;5(16):4245-53
16196095
Ann N Y Acad Sci. 1989;557:61-85; discussion 85-6
2472096
Comp Med. 2009 Dec;59(6):517-26
20034426
Vet J. 2000 Mar;159(2):154-60
10712803
Vet J. 2004 Jul;168(1):28-40
15158206
Clin Chim Acta. 1974 May 31;53(1):109-15
4135424
Vet Clin Pathol. 2001;30(4):170-176
12024297
Virology. 1995 Feb 20;207(1):287-91
7871740
J Immunol. 1965 Dec;95(6):1023-33
5852196
Vet Clin Pathol. 1986;15(4):20-5
15334365
Virology. 1990 Mar;175(1):211-21
1689918
Res Vet Sci. 2007 Feb;82(1):115-25
16759680
Comp Med. 2007 Feb;57(1):66-73
17348293
Vet Clin Pathol. 2007 Mar;36(1):64-72
17311197
Vet Med Small Anim Clin. 1979 Sep;74(9):1251-8
92848
Vet Immunol Immunopathol. 1998 Feb 27;61(2-4):291-304
9613442
Vet Rec. 2002 Feb 23;150(8):241-4
11916025