title: Regulation of muscle mass by follistatin and activins.
abstract: Myostatin is a TGF-β family member that normally acts to limit skeletal muscle mass. Follistatin is a myostatin-binding protein that can inhibit myostatin activity in vitro and promote muscle growth in vivo. Mice homozygous for a mutation in the Fst gene have been shown to die immediately after birth but have a reduced amount of muscle tissue, consistent with a role for follistatin in regulating myogenesis. Here, we show that Fst mutant mice exhibit haploinsufficiency, with muscles of Fst heterozygotes having significantly reduced size, a shift toward more oxidative fiber types, an impairment of muscle remodeling in response to cardiotoxin-induced injury, and a reduction in tetanic force production yet a maintenance of specific force. We show that the effect of heterozygous loss of Fst is at least partially retained in a Mstn-null background, implying that follistatin normally acts to inhibit other TGF-β family members in addition to myostatin to regulate muscle size. Finally, we present genetic evidence suggesting that activin A may be one of the ligands that is regulated by follistatin and that functions with myostatin to limit muscle mass. These findings potentially have important implications with respect to the development of therapeutics targeting this signaling pathway to preserve muscle mass and prevent muscle atrophy in a variety of inherited and acquired forms of muscle degeneration.
authors.affiliation: Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA. sjlee@jhmi.edu
authors.forename: Se-Jin
authors.initials: SJ
authors.lastname: Lee
authors.forename: Yun-Sil
authors.initials: YS
authors.lastname: Lee
authors.forename: Teresa A
authors.initials: TA
authors.lastname: Zimmers
authors.forename: Arshia
authors.initials: A
authors.lastname: Soleimani
authors.forename: Martin M
authors.initials: MM
authors.lastname: Matzuk
authors.forename: Kunihiro
authors.initials: K
authors.lastname: Tsuchida
authors.forename: Ronald D
authors.initials: RD
authors.lastname: Cohn
authors.forename: Elisabeth R
authors.initials: ER
authors.lastname: Barton
mesh_terms: D028341:Activins / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D038681:Follistatin / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009119:Muscle Contraction / Q000502:physiology; D024510:Muscle Development / Q000502:physiology; D018482:Muscle, Skeletal / Q000033:anatomy & histology* / Q000502:physiology; D055435:Myostatin / Q000235:genetics / Q000378:metabolism; D009929:Organ Size
chemical_list: D038681:Follistatin; D055435:Myostatin; D028341:Activins
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