[Biodegradation characteristics of o-chlorophenol with photosynthetic bacteria PSB-1D].
A strain of photosynthetic bacteria named PSB-1D with degradation of o-chlorophenol (2-CP) was isolated and screened from the shallow substrate sludge in downstream side of the sewage outfall of an insecticide factory. The PSB-1D is identified preliminarily as Rhodopseudomonas sp. according to its colony and cell morphological properties, physiological biochemical characteristics and absorption spectrum analysis of living cells. The experiments results of relationship between PSB-1D growth and o-chlorophenol degradation showed that the degradation rate of o-chlorophenol was up to 57.26% after 7 days cultural time. The main environmental factors including way of illumination and oxygen, initial pH, cultural temperature, illumination intensity had distinctly influenced on the o-chlorophenol degradation with PSB-1D. The results showed that the optimum conditions were as following: an anaerobic light, pH 7.0, temperature 30 degrees C, illumination intensity 4000 lx,initial o-chlorophenol concentration 50 mg/L. Under that cultural condition, the degradation rate of o-chlorophenol could reach to 62.08%. The degradation kinetic data fitted the Andrews model well. In addition, the biodegradation process of o-chlorophenol can be well described by enzymatic reaction of high concentration inhibition, with the maximum substrate utilization rate 0.309 d(-1), Michaelis-Menten constant 2.733 mg/L, inhibitory constant 230.15 mg/L respectively.
Hu
Xiao-min
XM
College of Resources and Civil Engineering, Northeastern University, Shenyang 110004, China. hxmin_jj@163.com
Dong
Yi-hu
YH
Li
Liang
L
Lu
Juan
J
He
Ying-dian
YD
Gao
Yang
Y
D001673:Biodegradation, Environmental; D002733:Chlorophenols / Q000302:isolation & purification* / Q000378:metabolism; D012241:Rhodopseudomonas / Q000302:isolation & purification* / Q000378:metabolism*; D012722:Sewage / Q000382:microbiology; D014865:Waste Disposal, Fluid / Q000379:methods; D014874:Water Pollutants, Chemical / Q000302:isolation & purification* / Q000378:metabolism
D002733:Chlorophenols; D012722:Sewage; D014874:Water Pollutants, Chemical; C030683:2-chlorophenol