Compounds having thiourea moiety as derivatization reagents in liquid chromatography/electrospray ionization-tandem mass spectrometry (LC/ESI-MS/MS): synthesis of derivatization reagents for carboxylic acids.
The derivatization reagents for carboxylic acids, N-(Pyridin-3-yl)hydrazinecarbothioamide, N-[4-(dimethylamino)phenyl]hydrazinecarbothioamide, 1-(2-aminoethyl)-3-(pyridin-3-yl)thiourea, 1-(2-aminoethyl)-3-[4-(dimethylamino)phenyl]thiourea and 4-(2-aminoethyl)-N-phenylpiperazine-1-carbothioamide were synthesized. These reagents reacted with carboxylic acids at 60°C for 45 min in the presence of the condensation reagents. The generated derivatives were favorably separated on the reversed-phase column and sensitively detected by electrospray ionization tandem mass spectrometry. These reagents enhanced the electrospray ionization response of the analyte and generated a particular product ion efficiently by collision-induced dissociation, and thus they were suitable for MS/MS detection.
Inoda
Hirotaka
H
Graduate School of Pharmaceutical Sciences, The University of Tokyo, Hongo, Bunkyo-ku, Japan.
Nishiyama
Taihei
T
Yoshikado
Takashi
T
Suwanai
Yusuke
Y
Santa
Tomofumi
T
D002264:Carboxylic Acids / Q000032:analysis / Q000737:chemistry*; D056148:Chromatography, Reverse-Phase; D007202:Indicators and Reagents / Q000138:chemical synthesis / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013890:Thiourea / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry
D002264:Carboxylic Acids; D007202:Indicators and Reagents; D013890:Thiourea