Engineered lactic acid bacterium Lactococcus lactis capable of binding antibodies and tumor necrosis factor alpha.
We have optimized the display of the B domain of staphylococcal protein A on the surface of Lactococcus lactis. The maximum binding capacity was estimated at 0.146 μg of antibody per 10⁸ cells and was sustained at 86% after treatment with simulated gastric juice. A tumor necrosis factor alpha (TNF-α)-binding affibody was also displayed and bound TNF-α, which could be useful in the treatment of inflammatory bowel disease.
Ravnikar
Matjaz
M
Department of Biotechnology, JoŽef Stefan Institute, Jamova 39, SI-1000 Ljubljana, Slovenia. ales.berlec@ijs.si.
Strukelj
Borut
B
Obermajer
Natasa
N
Lunder
Mojca
M
Berlec
Ales
A
D000906:Antibodies / Q000378:metabolism*; D013294:Lactococcus lactis / Q000235:genetics* / Q000378:metabolism*; D011485:Protein Binding; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013205:Staphylococcal Protein A / Q000235:genetics* / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D000906:Antibodies; D011994:Recombinant Proteins; D013205:Staphylococcal Protein A; D014409:Tumor Necrosis Factor-alpha
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