Comparative proteomic analysis of Typha angustifolia leaf under chromium, cadmium and lead stress.
The present study investigated Typha angustifolia leaf proteome in response to Cr, Cd and Pb stress. T. angustifolia of 90 (D90) and 130d (D130) old plants were subjected to 1mM Cr, Cd and Pb and samples were collected 30d after treatment. 2-DE coupled with MS (mass spectrometry) was used to analyze and identify Cr, Cd and Pb-responsive proteins. More than 1600 protein spots were reproducibly detected on each gel, wherein 44, 46, 66 and 33, 26, 62 spots in D90 and D130 samples were differentially expressed by Cr, Cd, Pb over the control, respectively. Of these differentially expressed proteins, 3, 1, 8 overlapped in D90 and D130; while 5, 8, 5 with regulation factors above 3 in one of D90 or D130 samples. Total of 22 and 4 up- and down-regulated proteins were identified using MS and data bank analysis. Cr-induced expression of ATP synthase, RuBisCO small subunit and coproporphyrinogen III oxidase; Cd-induced RuBisCO large subunit; Pb up-regulated carbohydrate metabolic pathway enzymes of fructokinase, and improved RuBisCO activase and large subunit, Mg-protoporphyrin IX chelatase. Contrarily, elF4F was inhibited by Cr/Pb, chloroplast FtsZ-like protein and GF14 omega impeded by Cd and Pb, respectively.
Bah
Alieu Mohamed
AM
Department of Agronomy, College of Agriculture and Biotechnology, Huajiachi Campus, Zhejiang University, Hangzhou 310029, China.
Sun
Hongyan
H
Department of Agronomy, College of Agriculture and Biotechnology, Huajiachi Campus, Zhejiang University, Hangzhou 310029, China.
Chen
Fei
F
Department of Agronomy, College of Agriculture and Biotechnology, Huajiachi Campus, Zhejiang University, Hangzhou 310029, China.
Zhou
Jing
J
Department of Agronomy, College of Agriculture and Biotechnology, Huajiachi Campus, Zhejiang University, Hangzhou 310029, China.
Dai
Huaxin
H
Department of Agronomy, College of Agriculture and Biotechnology, Huajiachi Campus, Zhejiang University, Hangzhou 310029, China.
Zhang
Guoping
G
Department of Agronomy, College of Agriculture and Biotechnology, Huajiachi Campus, Zhejiang University, Hangzhou 310029, China.
Wu
Feibo
F
Department of Agronomy, College of Agriculture and Biotechnology, Huajiachi Campus, Zhejiang University, Hangzhou 310029, China. Electronic address: wufeibo@zju.edu.cn.
D002104:Cadmium / Q000633:toxicity*; D002857:Chromium / Q000633:toxicity*; D015180:Electrophoresis, Gel, Two-Dimensional; D007854:Lead / Q000633:toxicity*; D018515:Plant Leaves / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry*; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013312:Stress, Physiological; D032401:Typhaceae / Q000737:chemistry*
D010940:Plant Proteins; D002104:Cadmium; D002857:Chromium; D007854:Lead