Application of bioluminescence imaging to therapeutic intervention of herpes simplex virus type I - Thymidine kinase/ganciclovir in glioma.
Lentiviral vector containing the HSV1-tk and firefly luciferase (fLuc) gene was infected into C6 and C6-TL expressing HSV1-tk and fLuc gene was generated. C6-TL showed higher [(125)I]IVDU uptake than C6. The survival rate of C6-TL decreased more rapidly with increasing GCV dose and was well correlated with fLuc activity. The images of microPET clearly demonstrated higher uptake of [(18)F]FHBG into the C6-TL tumor. Inhibition of tumor growth was observed in C6-TL tumor-bearing mice treated with GCV through tumor size measurement and bioluminescence imaging. The therapeutic effect of HSV1-tk/GCV system can be monitored using bioluminescent imaging and tumor size measurement.
Jang
Su Jin
SJ
Molecular Imaging Research Center, Korea Institute of Radiological and Medical Sciences, Seoul, Republic of Korea.
Kang
Joo Hyun
JH
Kim
Kwang Il
KI
Lee
Tae Sup
TS
Lee
Yong Jin
YJ
Lee
Kyo Chul
KC
Woo
Kwang Sun
KS
Chung
Wee Sup
WS
Kwon
Hee Chung
HC
Ryu
Chun Jeih
CJ
Choi
Tae Hyun
TH
Choi
Chang Woon
CW
Lim
Sang Moo
SM
Cheon
Gi Jeong
GJ
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001932:Brain Neoplasms / Q000201:enzymology / Q000235:genetics / Q000628:therapy*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005260:Female; D015774:Ganciclovir / Q000378:metabolism / Q000494:pharmacology*; D017930:Genes, Reporter; D041722:Genes, Transgenic, Suicide; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics; D005909:Glioblastoma / Q000201:enzymology / Q000235:genetics / Q000628:therapy*; D018259:Herpesvirus 1, Human / Q000201:enzymology* / Q000235:genetics; D007065:Idoxuridine / Q000031:analogs & derivatives / Q000378:metabolism; D007700:Kinetics; D016086:Lentivirus / Q000235:genetics; D049409:Luciferases, Firefly / Q000096:biosynthesis / Q000235:genetics; D008163:Luminescent Measurements; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D049268:Positron-Emission Tomography; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D013937:Thymidine Kinase / Q000096:biosynthesis* / Q000235:genetics; D047368:Tumor Burden / Q000187:drug effects
D000970:Antineoplastic Agents; D011993:Recombinant Fusion Proteins; C030360:5-(2-iodovinyl)-2'-deoxyuridine; D049409:Luciferases, Firefly; D013937:Thymidine Kinase; D007065:Idoxuridine; D015774:Ganciclovir