Effect of docetaxel on expression of eIF3a in human lung cancer A549 cell line.
OBJECTIVE
To explore the dose-dependent and time-dependent effect of docetaxel on the expression of mammalian eukaryotic initiation factor 3 subunit A (eIF3a) in lung cancer cell line.


METHODS
The human lung cancer cell line A549 was treated with gradient concentrations of docetaxel for different time. Real-time PCR and Western blot were used to detect mRNA and protein expression levels of eIF3a and alpha-tubulin, respectively.


RESULTS
Docetaxel did not affect alpha-tubulin expression at either mRNA level or protein level. When A549 cells were treated with high concentration of docetaxel (30 μg/L), the expression level of eIF3a mRNA tended to increase in a time-dependent manner. Protein expression level of alpha-tubulin was not associated with eIF3a expression significantly in cells treated by docetaxel.


CONCLUSIONS
Docetaxel could slightly increase the expression of eIF3a mRNA, and eIF3a does not regulate the expression of alpha-tubulin in A549 cells treated by docetaxel.
Gong
Zhicheng
Z
Institute of Clinical Pharmacology, Hunan Key Laboratory of Pharmacogenetics, Central South University, Changsha 410078, China.
Xu
Xiaojing
X
Hou
Ruiying
R
Guo
Yue
Y
Sheng
Feifeng
F
Ouyang
Dongsheng
D
Zhou
Honghao
H
Liu
Zhaoqian
Z
D000970:Antineoplastic Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D000077143:Docetaxel; D039621:Eukaryotic Initiation Factor-3 / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D043823:Taxoids / Q000494:pharmacology*; D014404:Tubulin / Q000235:genetics / Q000378:metabolism
D000970:Antineoplastic Agents; C105988:EIF3A protein, human; D039621:Eukaryotic Initiation Factor-3; D012333:RNA, Messenger; D043823:Taxoids; D014404:Tubulin; D000077143:Docetaxel