title: Hematoxylin-stainability of keratohyalin granules is due to the novel component, fibrinogen γ-chain protein.
abstract: Hematoxylin-stainability of keratohyalin granules (KHG) using biochemical and immunohistochemical techniques is due to the presence of a fibrinogen γ-chain protein. A protein with a molecular weight of 100 kDa was stained with anti-Ted-H-1 monoclonal antibody and hematoxylin solution (hematoxylin-stainable protein). Since the amino acid sequence of the hematoxylin-stainable protein was to that of fibrinogen γ-chain protein, a peptide was synthesized and an antibody against the peptide was produced. This antibody reacted with the hematoxylin-stainable protein and fibrinogen γ-chain protein on immunoblot analysis and with KHG on immunohistochemical examination. Furthermore, a commercial anti-fibrinogen γ-chain protein antibody (Ab) also reacted with the hematoxylin-stainable protein as well as fibrinogen. In contrast, anti-fibrinogen β-chain protein Ab did not react with the hematoxylin-stainable protein. The fibrinogen γ-chain protein also stained with hematoxylin. These findings suggested that fibrinogen γ-chain protein may be a novel component protein of KHG and may induce the hematoxylin-stainability of KHG.
authors.affiliation: Institute for Health Sciences, Tokushima Bunri University, Yamashiro, Tokushima, Tokushima, Japan. masaet@tokushima.bunri-u.ac.jp
authors.forename: Masae
authors.initials: M
authors.lastname: Takahashi
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Horiuchi
authors.forename: Tadashi
authors.initials: T
authors.lastname: Tezuka
mesh_terms: D000911:Antibodies, Monoclonal; D015415:Biomarkers / Q000378:metabolism; D003594:Cytoplasmic Granules / Q000378:metabolism* / Q000473:pathology; D003876:Dermatitis, Atopic / Q000175:diagnosis / Q000378:metabolism* / Q000473:pathology; D019281:Dimerization; D005340:Fibrinogen / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D019084:Fluorescent Antibody Technique, Indirect; D006416:Hematoxylin / Q000378:metabolism; D006801:Humans; D007633:Keratins / Q000378:metabolism; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000276:immunology / Q000378:metabolism*; D011485:Protein Binding; D020539:Sequence Analysis, Protein; D012867:Skin / Q000378:metabolism* / Q000473:pathology
chemical_list: D000911:Antibodies, Monoclonal; D015415:Biomarkers; D010446:Peptide Fragments; C027539:fibrinopeptides gamma; C001500:keratohyalin; D007633:Keratins; D005340:Fibrinogen; D006416:Hematoxylin