A DNA microarray for identification of selected Korean birds based on mitochondrial cytochrome c oxidase I gene sequences.
DNA barcoding with the gene encoding cytochrome c oxidase I (COI) in the mitochondrial genome has been proposed as a standard marker to identify and discover animal species. Some migratory wild birds are suspected of transmitting avian influenza and pose a threat to aircraft safety because of bird strikes. We have previously reported the COI gene sequences of 92 Korean bird species. In the present study, we developed a DNA microarray to identify 17 selected bird species on the basis of nucleotide diversity. We designed and synthesized 19 specific oligonucleotide probes; these probes were arrayed on a silylated glass slide. The length of the probes was 19-24 bps. The COI sequences amplified from the tissues of the selected birds were labeled with a fluorescent probe for microarray hybridization, and unique hybridization patterns were detected for each selected species. These patterns may be considered diagnostic patterns for species identification. This microarray system will provide a sensitive and a high-throughput method for identification of Korean birds.
Chung
In-Hyuk
IH
Department of Biochemistry, Hanyang University and GenoCheck Co. Ltd., Ansan, 426-791, Korea.
Yoo
Hye Sook
HS
Eah
Jae-Yong
JY
Yoon
Hyun-Kyu
HK
Jung
Jin-Wook
JW
Hwang
Seung Yong
SY
Kim
Chang-Bae
CB
D000818:Animals; D001483:Base Sequence; D001717:Birds / Q000145:classification / Q000235:genetics; D058893:DNA Barcoding, Taxonomic / Q000379:methods; D015342:DNA Probes / Q000138:chemical synthesis / Q000235:genetics; D004272:DNA, Mitochondrial / Q000737:chemistry / Q000235:genetics*; D003576:Electron Transport Complex IV / Q000737:chemistry / Q000235:genetics*; D050259:Genes, Mitochondrial; D014644:Genetic Variation; D005585:Influenza in Birds / Q000145:classification / Q000635:transmission; D007723:Korea; D008928:Mitochondria / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D015345:Oligonucleotide Probes / Q000138:chemical synthesis / Q000235:genetics; D013045:Species Specificity
D015342:DNA Probes; D004272:DNA, Mitochondrial; D015345:Oligonucleotide Probes; D003576:Electron Transport Complex IV