title: The catecholaminergic nerve plexus of Holothuroidea.
abstract: Catecholamines have been extensively reported to be present in most animal groups, including members of Echinodermata. In this study, we investigated the presence and distribution of catecholaminergic nerves in two members of the Holothuroidea, Holothuria glaberrima (Selenka, 1867) (Aspidochirotida, Holothuroidea) and Holothuria mexicana (Ludwig, 1875) (Aspidochirotida, Holothuroidea), by using induced fluorescence for catecholamines on tissue sections and immunohistochemistry with an antibody that recognizes tyrosine hydroxylase. The presence of a catecholaminergic nerve plexus similar in distribution and extension to those previously reported in other members of Echinodermata was observed. This plexus, composed of cells and fibers, is found in the ectoneural component of the echinoderm nervous system and is continuous with the circumoral nerve ring and the radial nerves, tentacular nerves, and esophageal plexus. In addition, fluorescent nerves in the tube feet are continuous with the catecholaminergic components of the radial nerve cords. This is the first comprehensive report on the presence and distribution of catecholamines in the nervous system of Holothuroidea. The continuity and distribution of the catecholaminergic plexus strengthen the notion that the catecholaminergic cells are interneurons, since these do not form part of the known sensory or motor circuits and the fluorescence is confined to organized nervous tissue.
authors.affiliation: Department of Biology, University of Puerto Rico, Río Piedras Campus, Box 23360, Río Piedras, PR 00931-3360, USA.
authors.forename: Carlos A
authors.initials: CA
authors.lastname: Díaz-Balzac
authors.forename: Wigberto
authors.initials: W
authors.lastname: Mejías
authors.forename: Luis B
authors.initials: LB
authors.lastname: Jiménez
authors.forename: José E
authors.initials: JE
authors.lastname: García-Arrarás
references.citation: Biol Bull. 2007 Aug;213(1):28-42
references.pmid: 17679718
references.citation: J Exp Biol. 2001 Mar;204(Pt 5):865-73
references.pmid: 11171410
references.citation: Br J Pharmacol Chemother. 1967 Jan;29(1):63-9
references.pmid: 19108240
references.citation: Biol Bull. 2009 Jun;216(3):322-34
references.pmid: 19556597
references.citation: Brain Res Dev Brain Res. 1995 Jul 14;87(2):160-71
references.pmid: 7586498
references.citation: Zoolog Sci. 2002 Nov;19(11):1217-22
references.pmid: 12499664
references.citation: Zoomorphology. 2010;129(1):33-43
references.pmid: 20461218
references.citation: Dev Biol. 2006 Apr 1;292(1):205-12
references.pmid: 16442090
references.citation: Cell Tissue Res. 1996 Dec;286(3):385-91
references.pmid: 8929341
references.citation: Neurochem Res. 2005 Jun-Jul;30(6-7):825-37
references.pmid: 16187217
references.citation: J Comp Neurol. 2009 Jun 1;514(4):329-42
references.pmid: 19330814
references.citation: Biol Rev Camb Philos Soc. 1972 Aug;47(3):363-92
references.pmid: 4563848
references.citation: J Histochem Cytochem. 1989 Aug;37(8):1197-205
references.pmid: 2569003
references.citation: J Comp Neurol. 1991 Jan 15;303(3):457-77
references.pmid: 1672535
references.citation: J Comp Neurol. 2008 Jul 20;509(3):271-82
references.pmid: 18473389
references.citation: J Comp Neurol. 2003 Aug 4;462(4):400-14
references.pmid: 12811809
references.citation: Z Zellforsch Mikrosk Anat. 1969;98(3):323-32
references.pmid: 4390792
references.citation: Comp Gen Pharmacol. 1970 Mar;1(1):73-81
references.pmid: 5527546
references.citation: Dev Genes Evol. 2007 Aug;217(8):585-92
references.pmid: 17622554
references.citation: J Comp Neurol. 1975 Sep 15;163(2):215-26
references.pmid: 240872
references.citation: Histochemistry. 1976 Oct 22;49(2):81-93
references.pmid: 825485
references.citation: J Comp Neurol. 1995 Feb 20;352(4):626-40
references.pmid: 7722004
references.citation: Brain Res Bull. 1982 Jul-Dec;9(1-6):27-31
references.pmid: 6756549
references.citation: J Comp Neurol. 1997 Mar 31;380(1):119-35
references.pmid: 9073087
references.citation: Cell Tissue Res. 1994 Oct;278(1):177-85
references.pmid: 7954698