Development and validation of a fast RP-HPLC method for the determination of clobetasol propionate in topical nanocapsule suspensions.
A simple and rapid high-performance liquid chromatographic method is validated for the determination of clobetasol propionate in topical nanocapsule suspensions. The method is carried out on an RP-18 column with a mobile phase composed of methanol-water (80:20 v/v) and UV detection at 241 nm. The method validation yields good results with respect to linearity, specificity, precision, accuracy, and robustness. The calibration curve in the range of 5.0-40.0 microg/mL shows a correlation coefficient of 0.9999. Precision (intra-day and inter-day) is demonstrated by a relative standard deviation lower than 1.5%. Accuracy is assessed by the recovery test of clobetasol propionate from sample matrixes (98.33 +/- 0.88%). In conclusion, the method is suitable to be applied to assay clobetasol propionate in topical formulations of polymeric nanocapsules, avoiding the use of a buffer solution in the mobile phase.
Fontana
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MC
Programa de Pós-Graduação em Ciências Farmacêuticas, Departamento de Farmácia Industrial, Av. Roraima, 1000, Universidade Federal de Santa Maria, Santa Maria, RS, 97105-900 Brazil.
Bastos
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MO
Beck
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RC
D000287:Administration, Topical; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D002990:Clobetasol / Q000032:analysis* / Q000737:chemistry; D016014:Linear Models; D053769:Nanocapsules / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D053769:Nanocapsules; D002990:Clobetasol