title: Characterization of Lactococcus lactis phage 949 and comparison with other lactococcal phages.
abstract: The virulent Lactococcus lactis phage 949 was isolated in 1975 from cheese whey in New Zealand. This phage is a member of the Siphoviridae family and of a rare lactococcal phage group that bears its name (949 group). It has an icosahedral capsid (79-nm diameter) and a very long noncontractile tail (length, 500 nm; width, 12 nm). It infected 7 of 59 tested L. lactis strains, a somewhat expanded host range for a rare lactococcal phage. The abortive phage infection defense mechanisms AbiQ and AbiT strongly inhibited the multiplication of phage 949, but AbiK and AbiV did not. Its double-stranded DNA (dsDNA) genome of 114,768 bp is, to date, the largest among lactococcal phages. Its GC content was calculated at 32.7%, which is the lowest reported for a lactococcal phage. Its 154 open reading frames (ORFs) share limited identity with database sequences. In addition, terminal redundancy was observed as well as the presence of six tRNAs, one group I intron, and putative recombinases. SDS-PAGE coupled with mass spectrometry identified 13 structural proteins. The genomes of the members of the 10 currently known L. lactis phage groups were used to construct a proteomic tree. Each L. lactis phage group separated into distinct genetic clusters, validating the current classification scheme. Of note, members of the polythetic P335 groups were clearly separated into subgroups.
authors.affiliation: Groupe de Recherche en Écologie Buccale, Faculté de Médecine Dentaire, Université Laval, Quebec City, Quebec G1V0A6, Canada.
authors.forename: Julie E
authors.initials: JE
authors.lastname: Samson
authors.forename: Sylvain
authors.initials: S
authors.lastname: Moineau
mesh_terms: D000818:Animals; D001435:Bacteriophages / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000648:ultrastructure*; D001482:Base Composition; D002611:Cheese; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D023061:Gene Order; D058507:Host Specificity; D013294:Lactococcus lactis / Q000821:virology*; D013058:Mass Spectrometry; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D009520:New Zealand; D016366:Open Reading Frames; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D017906:Siphoviridae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000648:ultrastructure; D014764:Viral Proteins / Q000032:analysis; D014771:Virion / Q000648:ultrastructure
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins; D004247:DNA
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