Morin (2',3,4',5,7-pentahydroxyflavone) protected cells against γ-radiation-induced oxidative stress.
Ionizing radiation can induce cell damage by generating reactive oxygen species (ROS). The present study was carried out to investigate the radio-protective effects of a flavonoid compound, morin (2',3,4',5,7-pentahydroxyflavone) and the underlying mechanisms. Morin was found to reduce the intracellular ROS generated by γ-irradiation. Moreover, morin protected cellular components against radiation-induced membrane lipid peroxidation and cellular DNA damage, which are the main targets of radiation-induced cell damage. Morin recovered cell viability damaged by radiation via inhibition of apoptosis. Irradiated cells with morin treatment reduced Bax, phospho Bcl-2, active caspase 9 and caspase 3, which were induced by γ-radiation. Irradiated cells with morin recovered the expression of Bcl-2 reduced by γ-radiation. Morin exerted anti-apoptotic effects via inhibition of mitogen-activated protein kinase kinase-4 (MKK4/SEK1)-c-Jun NH(2) -terminal kinase (JNK)-activator protein 1 (AP-1) cascades induced by γ-radiation. The results suggest that morin protects cells against oxidative stress induced by radiation via reduction of ROS and attenuation of SEK1-JNK-AP-1 pathway.
Zhang
Rui
R
Department of Biochemistry, College of Medicine and Applied Radiological Science Research Institute, Jeju National University, Jeju-si, Korea.
Kang
Kyoung Ah
KA
Kang
Sam Sik
SS
Park
Jae Woo
JW
Hyun
Jin Won
JW
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D006224:Cricetinae; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D005419:Flavonoids / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000494:pharmacology*; D005720:Gamma Rays; D008168:Lung / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D048670:MAP Kinase Kinase 4 / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000633:toxicity; D015398:Signal Transduction / Q000187:drug effects; D018808:Transcription Factor AP-1 / Q000378:metabolism; D047309:Flavones
D005419:Flavonoids; D016166:Free Radical Scavengers; D048670:MAP Kinase Kinase 4; D011837:Radiation-Protective Agents; D017382:Reactive Oxygen Species; D018808:Transcription Factor AP-1; C008548:morin; D047309:Flavones