Time of sampling is crucial for measurement of cell-free plasma DNA following acute aseptic inflammation induced by exercise.
OBJECTIVE
To determine the time-course changes of cell-free plasma DNA (cfDNA) following heavy exercise.


METHODS
cfDNA concentration, C-reactive protein levels (hs-CRP), uric acid concentration (UA), creatine kinase activity (CK) were measured before and post-exercise (immediately post, 0.5h, 1h, 2h, 3h, 4h, 5h, 6h, 8h, 10h, 24h).


RESULTS
cfDNA increased (15-fold) 30-min post-exercise and normalized thereafter. hs-CRP increased (56%, p<0.001) 1h post-exercise, remained elevated throughout recovery (52-142%, p<0.0001), and peaked (200% rise, p<0.0001) at 24h post-exercise. UA and CK increased (p<0.05), immediately post-exercise, remained elevated throughout recovery (p<0.0001), and peaked (p<0.0001) at 24h of post-exercise recovery.


CONCLUSIONS
cfDNA sampling timing is crucial and a potential source of error following aseptic inflammation.
Fatouros
Ioannis G
IG
Department of Physical Education and Sports Science, Democritus University of Thrace, Komotini 69100, Greece. ifatouro@phyed.duth.gr
Jamurtas
Athanasios Z
AZ
Nikolaidis
Michalis G
MG
Destouni
Aspasia
A
Michailidis
Yiannis
Y
Vrettou
Christina
C
Douroudos
Ioannis I
II
Avloniti
Alexandra
A
Chatzinikolaou
Athanasios
A
Taxildaris
Kiriakos
K
Kanavakis
Emmanouel
E
Papassotiriou
Ioannis
I
Kouretas
Dimitrios
D
D000208:Acute Disease; D001207:Asepsis; D002097:C-Reactive Protein / Q000378:metabolism; D002474:Cell-Free System; D003402:Creatine Kinase / Q000097:blood; D004247:DNA / Q000097:blood*; D015444:Exercise / Q000502:physiology*; D006801:Humans; D007249:Inflammation / Q000097:blood* / Q000503:physiopathology*; D008297:Male; D012146:Rest / Q000502:physiology; D013048:Specimen Handling / Q000379:methods*; D013997:Time Factors; D014527:Uric Acid / Q000097:blood; D055815:Young Adult
D014527:Uric Acid; D002097:C-Reactive Protein; D004247:DNA; D003402:Creatine Kinase