title: Members of the YjgF/YER057c/UK114 family of proteins inhibit phosphoribosylamine synthesis in vitro.
abstract: The YjgF/YER057c/UK114 family of proteins is highly conserved across all three domains of life and currently lacks a consensus biochemical function. Analysis of Salmonella enterica strains lacking yjgF has led to a working model in which YjgF functions to remove potentially toxic secondary products of cellular enzymes. Strains lacking yjgF synthesize the thiamine precursor phosphoribosylamine (PRA) by a TrpD-dependent mechanism that is not present in wild-type strains. Here, PRA synthesis was reconstituted in vitro with anthranilate phosphoribosyltransferase (TrpD), threonine dehydratase (IlvA), threonine, and phosphoribosyl pyrophosphate. TrpD-dependent PRA formation in vitro was inhibited by S. enterica YjgF and the human homolog UK114. Thus, the work herein describes the first biochemical assay for diverse members of the highly conserved YjgF/YER057c/UK114 family of proteins and provides a means to dissect the cellular functions of these proteins.
authors.affiliation: Department of Bacteriology, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.
authors.forename: Jennifer A
authors.initials: JA
authors.lastname: Lambrecht
authors.forename: Beth Ann
authors.initials: BA
authors.lastname: Browne
authors.forename: Diana M
authors.initials: DM
authors.lastname: Downs
mesh_terms: D000877:Anthranilate Phosphoribosyltransferase / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism; D008954:Models, Biological; D010754:Phosphoribosyl Pyrophosphate / Q000235:genetics / Q000378:metabolism; D012260:Ribonucleases / Q000235:genetics / Q000378:metabolism; D012267:Ribosemonophosphates / Q000096:biosynthesis* / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D019779:Salmonella enterica / Q000235:genetics / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D013913:Threonine Dehydratase / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; C465442:HMF1 protein, S cerevisiae; D006360:Heat-Shock Proteins; D024101:Mitochondrial Proteins; D012267:Ribosemonophosphates; D029701:Saccharomyces cerevisiae Proteins; C008891:phosphoribosylamine; D010754:Phosphoribosyl Pyrophosphate; D000877:Anthranilate Phosphoribosyltransferase; D012260:Ribonucleases; C490788:RIDA protein, human; D013913:Threonine Dehydratase
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