Effects of protocatechuic acid on trans fat induced hepatic steatosis in mice.
The effects of protocatechuic acid (PCA) on hepatic activity and/or mRNA expression of lipogenic enzymes and sterol regulatory element-binding proteins (SREBPs) in mice fed a trans fatty acid (TFA)-rich diet were examined. PCA at 1, 2, or 4% was provided for 10 weeks. Results showed that TFA diet significantly enhanced hepatic activity and mRNA expression of fatty acid synthase, 3-hydroxy-3-methylglutaryl coenzyme A reductase, stearoyl-CoA desaturase-1, and SREBP-1c (P<0.05); however, the intake of PCA significantly diminished the activity and mRNA expression of these lipogenic factors and decreased hepatic lipid accumulation (P<0.05). TFA diet significantly increased hepatic levels of TFA and pro-inflammatory cytokines (P<0.05). However, PCA intake significantly lowered hepatic content of 18:1 trans and 18:2 trans, as well as reduced the level of test cytokines (P<0.05). These results indicate that PCA is a potent agent for attenuating TFA-induced hepatic steatosis.
Liu
Wen-Hu
WH
Radiation Safety Office, Chung Shan Medical University, Taichung City, Taiwan.
Lin
Chun-Che
CC
Wang
Zhi-Hong
ZH
Mong
Mei-Chin
MC
Yin
Mei-Chin
MC
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000627:therapeutic use*; D004305:Dose-Response Relationship, Drug; D005234:Fatty Liver / Q000097:blood / Q000378:metabolism / Q000517:prevention & control*; D062385:Hydroxybenzoates / Q000627:therapeutic use*; D006949:Hyperlipidemias / Q000097:blood / Q000517:prevention & control; D000960:Hypolipidemic Agents / Q000627:therapeutic use*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D044242:Trans Fatty Acids / Q000633:toxicity*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D062385:Hydroxybenzoates; D000960:Hypolipidemic Agents; D044242:Trans Fatty Acids; C009091:protocatechuic acid