title: Comparison of three methods for the estimation of the pituitary gland volume using magnetic resonance imaging: a stereological study.
abstract: Stereological techniques using point counting and planimetry have been used to estimate pituitary gland volume. However, many studies have estimated pituitary gland volume by the mathematical approach the elliptic formula. The objective of the current study was to determine pituitary gland volume using stereological methods and elliptic formula on magnetic resonance imaging (MRI). In this study, pituitary gland volumes were estimated in a total of 28 subjects (22 females, 6 males,) who were free of any pituitary or neurological symptoms and signs. The mean ± SD pituitary gland volumes for the point counting, planimetry and elliptic formulae groups were 582.14 ± 140.16 mm³, 610.08 ± 133.17 mm³, and 432.82 ± 147.38 mm³, respectively. The mean CE for the pituitary gland volume estimates derived from the point counting technique was 8.07%. No significant difference was found between point counting and planimetric methods for the pituitary gland volume (P > 0.05). In addition, there was a 26.14 and 29.71% underestimation of pituitary volume as measured by the elliptic formula compared to the point counting and planimetric techniques, respectively. From these results, it can be concluded that stereological techniques are unbiased, efficient and reliable methods and are ideally suitable for in vivo examination of MRI data for pituitary gland volume estimation. Hence, we suggest that estimating pituitary gland volume using MRI study and stereology may be clinically relevant for pituitary surgeons for the investigation of the structure and function of the pituitary gland.
authors.affiliation: Department of Anatomy, Erciyes University School of Medicine, Kayseri, Turkey. tertekin@erciyes.edu.tr
authors.forename: Tolga
authors.initials: T
authors.lastname: Ertekin
authors.forename: Niyazi
authors.initials: N
authors.lastname: Acer
authors.forename: Ahmet T
authors.initials: AT
authors.lastname: Turgut
authors.forename: Kenan
authors.initials: K
authors.lastname: Aycan
authors.forename: Ozlem
authors.initials: O
authors.lastname: Ozçelik
authors.forename: Mehmet
authors.initials: M
authors.lastname: Turgut
mesh_terms: D000328:Adult; D000368:Aged; D005260:Female; D006801:Humans; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D008875:Middle Aged; D010902:Pituitary Gland / Q000033:anatomy & histology*
references.citation: Pediatr Radiol. 2004 Jul;34(7):547-51
references.pmid: 15114413
references.citation: Pediatr Radiol. 1991;21(4):247-9
references.pmid: 1870916
references.citation: Biol Psychiatry. 2001 Aug 15;50(4):271-80
references.pmid: 11522262
references.citation: APMIS. 1988 May;96(5):379-94
references.pmid: 3288247
references.citation: J Craniofac Surg. 2009 May;20(3):921-5
references.pmid: 19461332
references.citation: Radiology. 1999 Nov;213(2):383-8
references.pmid: 10551216
references.citation: Pediatr Radiol. 1990;20(4):229-35
references.pmid: 2336284
references.citation: Clin Radiol. 1987 Jul;38(4):435-7
references.pmid: 3621827
references.citation: J Clin Endocrinol Metab. 1991 Feb;72(2):256-9
references.pmid: 1991797
references.citation: Pediatrics. 1994 Mar;93(3):409-16
references.pmid: 8115199
references.citation: Magn Reson Imaging. 2000 Dec;18(10):1187-99
references.pmid: 11167039
references.citation: Acta Radiol. 2010 Feb;51(1):71-7
references.pmid: 20001472
references.citation: Surg Radiol Anat. 2008 Jun;30(4):335-9
references.pmid: 18292960
references.citation: J Pediatr Endocrinol Metab. 2001 May;14(5):517-23
references.pmid: 11393572
references.citation: Br J Radiol. 2000 Jul;73(871):679-97
references.pmid: 11089458
references.citation: Radiology. 1990 Mar;174(3 Pt 1):681-5
references.pmid: 2305049
references.citation: Neuroimage. 2003 Feb;18(2):505-16
references.pmid: 12595203
references.citation: J Microsc. 2000 Sep;199 (Pt 3):224-38
references.pmid: 10971803
references.citation: AJR Am J Roentgenol. 1994 May;162(5):1167-73
references.pmid: 8166005
references.citation: Psychiatry Res. 2009 Apr 30;172(1):55-60
references.pmid: 19239986
references.citation: Radiology. 1989 Jul;172(1):171-7
references.pmid: 2740500
references.citation: Schizophr Res. 2007 Feb;90(1-3):266-73
references.pmid: 17187962
references.citation: J Microsc. 1999 Mar;193(Pt 3):199-211
references.pmid: 10348656
references.citation: Eur Arch Psychiatry Clin Neurosci. 2008 Sep;258(6):357-62
references.pmid: 18437280
references.citation: J Neurosci Methods. 2009 Apr 30;179(1):71-7
references.pmid: 19428514
references.citation: AJNR Am J Neuroradiol. 1985 Sep-Oct;6(5):717-22
references.pmid: 3933294
references.citation: AJNR Am J Neuroradiol. 1998 Sep;19(8):1495-9
references.pmid: 9763384
references.citation: Psychiatry Clin Neurosci. 2009 Aug;63(4):516-20
references.pmid: 19497000
references.citation: J Microsc. 2006 Jun;222(Pt 3):158-65
references.pmid: 16872414
references.citation: J Affect Disord. 2010 Aug;124(3):256-61
references.pmid: 20022640
references.citation: AJNR Am J Neuroradiol. 1983 May-Jun;4(3):411-4
references.pmid: 6410758
references.citation: AJNR Am J Neuroradiol. 1992 Sep-Oct;13(5):1295-9
references.pmid: 1414818
references.citation: Eur J Radiol. 2006 Jan;57(1):96-101
references.pmid: 16112829
references.citation: Brain Res. 2009 Jun 18;1276:77-82
references.pmid: 19393230
references.citation: Int J Gynaecol Obstet. 2003 Jul;82(1):25-30
references.pmid: 12834938
references.citation: Braz J Psychiatry. 2008 Mar;30(1):50-4
references.pmid: 18373019
references.citation: Am J Med. 1988 Aug;85(2):217-20
references.pmid: 3400697
references.citation: Neuropsychopharmacology. 2005 Oct;30(10):1923-31
references.pmid: 15956995
references.citation: Am J Roentgenol Radium Ther Nucl Med. 1962 Jun;87:989-1008
references.pmid: 13885978
references.citation: AJNR Am J Neuroradiol. 2000 Apr;21(4):685-9
references.pmid: 10782778
references.citation: Ann Plast Surg. 2007 Jan;58(1):48-53
references.pmid: 17197941
references.citation: J Clin Endocrinol Metab. 1990 Aug;71(2):505-8
references.pmid: 2380345
references.citation: J Microsc. 1987 Sep;147(Pt 3):229-63
references.pmid: 3430576
references.citation: Endocrinol Metab Clin North Am. 1991 Mar;20(1):165-82
references.pmid: 2029886