Molecular cloning and characterization of an achene-seed-specific promoter from motherwort (Leonurus japonicus Houtt).
LJAMP1 is a small antimicrobial protein purified previously from the seeds of motherwort, and it is expressed preferentially in seeds. A 794-bp upstream sequence of the ATG start codon was isolated using a genome walking method and cloned into the upstream of the β-glucuronidase (GUS) reporter gene to determine the GUS tissue-specific expression pattern. The transgenic tobacco showed that pLJAMP1 promoter derived GUS reporter gene special expression in pollen, achene and seed. The analysis of cis-acting elements also revealed pLJAMP1 promoter contained pollen and seed related transcriptional control elements.
Xie
Chengjian
C
Key Laboratory of Eco-Environments in Three Gorges Reservoir Region (Ministry of Education) and the School of Life Science, Southwest University, 400715, Chongqing, China.
Zhang
Beibei
B
Wang
De
D
Kou
Fei
F
Zhao
Xupeng
X
Yang
Xingyong
X
D019677:Artificial Gene Fusion; D003001:Cloning, Molecular; D017930:Genes, Reporter / Q000235:genetics; D005966:Glucuronidase / Q000096:biosynthesis / Q000235:genetics; D031332:Leonurus / Q000235:genetics*; D011058:Pollen / Q000235:genetics; D011401:Promoter Regions, Genetic; D050436:Regulatory Elements, Transcriptional; D012639:Seeds / Q000235:genetics*; D014026:Nicotiana / Q000235:genetics
D005966:Glucuronidase