Oxidative stress: a potential recipe for anxiety, hypertension and insulin resistance.
We recently reported involvement of oxidative stress in anxiety-like behavior of rats. Others in separate studies have demonstrated a link between oxidative stress and hypertension as well as with type 2 diabetes/insulin resistance. In the present study, we have tested a putative role of oxidative stress in anxiety-like behavior, hypertension and insulin resistance using a rat model of oxidative stress. Oxidative stress in rats was produced by xanthine (0.1%; drinking water) and xanthine oxidase (5 U/kg; i.p.). X+XO-treated rats had increased plasma and urinary 8-isoprostane levels (a marker of oxidative stress) and increased malondialdehyde (MDA) levels in the hippocampus and amygdala as compared to control rats. Serum corticosterone (a systemic marker of stress and anxiety) levels also increased with X+XO treatment. Moreover, anxiety-like behavior measured via open-field and light-dark exploration behavior tests significantly increased in X+XO-treated rats. Mean arterial blood pressure measured in anesthetized rats increased in X+XO-treated compared to control rats. Furthermore, plasma insulin but not glucose levels together with homeostasis model assessment (HOMA), an index of insulin resistance, were higher in X+XO-treated rats. Our studies suggest that oxidative stress is a common factor that link anxiety-like behavior, hypertension and insulin resistance in rats.
Salim
Samina
S
Department of Pharmacological and Pharmaceutical Sciences, University of Houston, TX, USA. ssalim@uh.edu
Asghar
Mohammad
M
Chugh
Gaurav
G
Taneja
Manish
M
Xia
Zhilian
Z
Saha
Kaustav
K
D000818:Animals; D001007:Anxiety / Q000503:physiopathology*; D006973:Hypertension / Q000503:physiopathology*; D007333:Insulin Resistance / Q000502:physiology*; D008297:Male; D018384:Oxidative Stress / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D019820:Xanthine / Q000633:toxicity; D014969:Xanthine Oxidase / Q000633:toxicity
D019820:Xanthine; D014969:Xanthine Oxidase
Am J Physiol Renal Physiol. 2009 Dec;297(6):F1543-9
19794106
J Altern Complement Med. 2008 Apr;14(3):303-7
18399759
Hypertension. 2007 Mar;49(3):664-71
17200436
Neurotoxicology. 2007 Nov;28(6):1191-9
17727954
Physiol Behav. 2009 Mar 2;96(3):421-7
19061907
Mol Cell Biochem. 2007 Oct;304(1-2):93-9
17503004
Pharmacol Biochem Behav. 1980 Aug;13(2):167-70
6106204
Discov Med. 2010 Feb;9(45):112-8
20193636
Behav Brain Res. 2010 Apr 2;208(2):545-52
20064565
Eur J Pharmacol. 2007 Jun 14;564(1-3):146-9
17395178
Anal Biochem. 1978 May;86(1):271-8
655387
Methods Enzymol. 2004;390:83-99
15488172
Gen Hosp Psychiatry. 2008 May-Jun;30(3):208-25
18433653
Curr Med Chem. 2009;16(23):2965-76
19689277
Learn Mem. 1998 Sep-Oct;5(4-5):302-16
10454356
J Clin Biochem Nutr. 2009 Sep;45(2):207-13
19794930
Am J Physiol Renal Physiol. 2008 Sep;295(3):F698-706
18614617
Behav Brain Res. 2008 Nov 21;193(2):277-88
18590774
Arch Gen Psychiatry. 1995 Oct;52(10):850-6
7575105
J Neurosci Methods. 1980 Dec;3(2):129-49
6110810
Hypertension. 2008 Feb;51(2):367-75
18158345
J Postgrad Med. 2002 Oct-Dec;48(4):317-21
12571395
Nature. 2005 Dec 1;438(7068):662-6
16244648
Free Radic Biol Med. 1994 Nov;17(5):429-37
7835749
Nat Med. 2005 Dec;11(12):1281-2
16333265
Diabet Med. 2010 Feb;27(2):217-24
20546267
Nutrition. 2001 Oct;17(10):888-95
11684397
J Psychosom Res. 2007 Jan;62(1):1-8
17188114
Life Sci. 2007 Jun 27;81(3):198-203
17574275
Anal Biochem. 1985 Oct;150(1):76-85
3843705
Brain Behav Immun. 2006 Jan;20(1):57-63
16364817
Eur J Clin Invest. 2003 Mar;33(3):231-8
12641541
Curr Hypertens Rep. 2010 Aug;12(4):243-51
20582734
Hypertension. 2008 Dec;52(6):1099-105
18955661
Diabetes. 2005 Jul;54(7):2219-26
15983225
Br J Pharmacol. 1999 Dec;128(7):1570-6
10602338
J Pharmacol Exp Ther. 2008 Aug;326(2):369-79
18456873
Diabetes Care. 2003 Aug;26(8):2311-7
12882854
Genes Brain Behav. 2009 Jun;8(4):398-406
19220484
J Affect Disord. 2010 Dec;127(1-3):321-5
20547423