Characterization of nanoprobe uptake in single cells: spatial and temporal tracking via SERS labeling and modulation of surface charge.
A critical aspect for use of nanoprobes in biomedical research and clinical applications involves fundamental spatial and temporal characterization of their uptake and distribution in cells. Raman spectroscopy and two-dimensional Raman imaging were used to identify and locate nanoprobes in single cells using surface-enhanced Raman scattering detection. To study the efficiency of cellular uptake, silver nanoparticles functionalized with three different positive-, negative-, and neutrally charged Raman labels were co-incubated with cell cultures and internalized via normal cellular processes. The surface charge on the nanoparticles was observed to modulate uptake efficiency, demonstrating a dual function of the surface modifications as tracking labels and as modulators of cell uptake. These results indicate that the functionalized nanoparticle construct has potential for sensing and delivery in single living cells and that use of surface-enhanced Raman scattering for tracking and detection is a practical and advantageous alternative to traditional fluorescence methods.


BACKGROUND
Cell labeling and tracking methods are commonly required in biomedical research. This paper presents specific functionalized nanoparticle constructs with potential for sensing and delivery in single living cells. The use of surface-enhanced Raman scattering enables tracking and detection of these cells as a practical alternative to traditional fluorescence methods.
Gregas
Molly K
MK
Department of Biomedical Engineering, Duke University, Durham, North Carolina, USA.
Yan
Fei
F
Scaffidi
Jonathan
J
Wang
Hsin-Neng
HN
Vo-Dinh
Tuan
T
D000818:Animals; D002460:Cell Line; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry*; D012834:Silver / Q000737:chemistry*; D013059:Spectrum Analysis, Raman / Q000379:methods*
D012834:Silver
Appl Spectrosc. 2006 Jan;60(1):1-8
16454901
Biophys J. 2003 Jun;84(6):3968-81
12770902
Langmuir. 2006 Sep 12;22(19):8178-85
16952259
Trends Analyt Chem. 2008 May;27(5):394-406
32287533
Anal Chem. 2003 Nov 15;75(22):6196-201
14616001
Phys Rev Lett. 1996 Apr 1;76(14):2444-2447
10060701
Toxicol Appl Pharmacol. 2009 Aug 1;238(3):280-8
19379767
Small. 2006 Mar;2(3):394-400
17193058
J Vet Sci. 2006 Dec;7(4):321-6
17106221
Nano Lett. 2006 Oct;6(10):2225-31
17034088
Exp Cell Res. 2002 Nov 15;281(1):86-100
12441132
J Nanosci Nanotechnol. 2002 Dec;2(6):653-8
12908430
Anal Bioanal Chem. 2009 May;394(1):85-94
19266187
Nano Lett. 2006 Apr;6(4):662-8
16608261
Lancet Oncol. 2006 Aug;7(8):657-67
16887483
Bioconjug Chem. 2004 May-Jun;15(3):482-90
15149175