Dehydroepiandrosterone ameliorates hepatocellular damage in obstructive jaundice.
We aimed to investigate the ameliorating effect of dehydroepiandrosterone (DHEA) on the potential hepatocellular damage in experimental obstructive jaundice. Twenty-four male rabbits in the study were randomly allocated into three groups. In the sham group, the choledochal canal was identified and explored. In the obstructive jaundice and treatment groups, the choledochal canal was ligated. Placebo and DHEA were administered to the obstructive jaundice and treatment groups, respectively. Blood samples were obtained at baseline, and both blood samples and liver tissue samples were obtained by re-laparotomy performed on day 8. Biochemical parameters were measured in blood samples, and liver samples were histopathologically evaluated. Alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma glutamyl transferase (GGT), alkaline phosphatase (ALP) and bilirubin levels were lower in the treatment group than in obstructive jaundice. Mononuclear inflammation in the portal region and hepatocyte degeneration were milder in the treatment group compared to obstructive jaundice group. Fibrosis and necrosis were also recovered by the DHEA treatment.In conclusion, these findings suggested that DHEA may reduce the obstructive jaundice-induced hepatocellular damage.
Gümüş
Metehan
M
Department of General Surgery, Medical Faculty, Dicle University, Diyarbakir, Turkey. metehangumus@yahoo.com
Celebi
Fehmi
F
Böyük
Abdullah
A
Gürsan
Nesrin
N
Akçay
Fatih
F
D000410:Alanine Transaminase / Q000378:metabolism; D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism; D001663:Bilirubin / Q000378:metabolism; D003687:Dehydroepiandrosterone / Q000008:administration & dosage*; D004195:Disease Models, Animal; D006801:Humans; D041781:Jaundice, Obstructive / Q000188:drug therapy* / Q000201:enzymology / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D011817:Rabbits; D011897:Random Allocation; D005723:gamma-Glutamyltransferase / Q000378:metabolism
D003687:Dehydroepiandrosterone; D005723:gamma-Glutamyltransferase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D001663:Bilirubin