[Effect of NAD+ against radiation injury and its dose-effect relationship].
OBJECTIVE
To explore the effect of NAD+ against radiation injury and its dose-effect relationship.


METHODS
L02 liver cells cultured in RPMI 1640 medium containing 10% fetal calf serum were exposed to X-ray irradiation followed by immediate application of NAD+. The cellular viability was analyzed by MTT assay and the apoptotic cells were detected by TUNEL methods to observe the damages of L02 liver cells induced by X-ray exposure and analyze the dose-effect relationship of NAD+.


RESULTS
The viability of L02 liver cells was decreased with increasing dose of X-ray irradiation. The most obvious growth inhibition of L02 cells occurred 24 h after the irradiation. NAD+ significantly increased the cell survival rate after irradiation, and this effect was gradually increased within the concentration range of 100-1000 microg/ml; at higher concentrations, the survival rate of the irradiated L02 cells showed no significant increase.


CONCLUSIONS
NAD+ provides partial protection of the liver cells against radiation injury, and the effect is positively correlated to NAD+ concentration within a certain range.
Li
Min-ying
MY
Department of Radiotherapy, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. lmy.69@163.com
Lei
Feng
F
You
Wei
W
Tan
Yu-jing
YJ
Lu
Xiao-jun
XJ
Chen
Long-hua
LH
Zhang
Ji-ren
JR
D017209:Apoptosis / Q000187:drug effects* / Q000528:radiation effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects* / Q000528:radiation effects; D004305:Dose-Response Relationship, Drug; D022781:Hepatocytes / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects; D006801:Humans; D009243:NAD / Q000008:administration & dosage / Q000494:pharmacology*; D011832:Radiation Injuries / Q000517:prevention & control
D009243:NAD