Isolectin B₄binding in populations of rat trigeminal ganglion cells.
We have recently classified dissociated trigeminal ganglion cells into nine types using electrophysiological current signatures. In the present study, we investigated the relationship between isolectin B(4) (IB(4)) binding and the cell types in rat trigeminal ganglion cells. We found that IB(4) was bound to all type 2 cells and more than 70% of cell types 1 and 13; however, it was bound to less than 20% of cell types 7 and 8 and did not bind at all to cell types 3-5 and 9. Thus, each trigeminal ganglion cell type showed high homogeneity in IB(4) binding. These results correspond to reported IB(4) binding profiles in the matched dorsal root ganglion cell types, except for types 5 and 7.
Ono
Kentaro
K
Department of Biosciences, Kyushu Dental College, 2-6-1 Manazuru, Kokurakitaku, Kitakyushu 803-8580, Japan. ono@kyu-dent.ac.jp
Xu
Shenghong
S
Inenaga
Kiyotoshi
K
D000818:Animals; D001665:Binding Sites / Q000187:drug effects / Q000502:physiology; D002211:Capsaicin / Q000494:pharmacology; D002478:Cells, Cultured; D031295:Griffonia / Q000737:chemistry / Q000378:metabolism*; D008297:Male; D009619:Nociceptors / Q000145:classification / Q000187:drug effects / Q000378:metabolism; D037121:Plant Lectins / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D011984:Sensory Receptor Cells / Q000145:classification / Q000187:drug effects / Q000378:metabolism*; D018689:Sensory System Agents / Q000494:pharmacology; D012668:Trigeminal Ganglion / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*
C045475:Griffonia simplicifolia lectins; D037121:Plant Lectins; D018689:Sensory System Agents; D002211:Capsaicin