Biological activity of heterologous murine interleukin-10 and preliminary studies on the use of a dextrin nanogel as a delivery system.
Interleukin-10 (IL-10) is an anti-inflammatory cytokine, which active form is a non-covalent homodimer with two intramolecular disulphide bonds essential for its biological activity. A mutated form of murine IL-10 was successfully expressed in E. coli, recovered and purified from inclusion bodies. Its ability to reduce tumor necrosis factor α synthesis and down-regulate class II major histocompatibility complex molecules expression on endotoxin-stimulated bone marrow-derived macrophages was confirmed, and shown to be similar to that of a commercially available IL-10. Given the potential of IL-10 for application in various medical conditions, it is essential to develop systems that can effectively deliver the protein. In this work it is shown that a dextrin nanogel effectively incorporate IL-10, stabilize, and enable the slow release of biologically active IL-10 over time. Altogether, these results demonstrate the suitability of dextrin nanogel to be used as a system for the controlled release of IL-10.
Carvalho
Vera
V
Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710-057, Braga, Portugal.
Castanheira
Pedro
P
Faria
Tiago Q
TQ
Gonçalves
Catarina
C
Madureira
Pedro
P
Faro
Carlos
C
Domingues
Lucília
L
Brito
Rui M M
RM
Vilanova
Manuel
M
Gama
Miguel
M
D000818:Animals; D003692:Delayed-Action Preparations; D003912:Dextrins / Q000737:chemistry*; D015536:Down-Regulation; D004337:Drug Carriers / Q000737:chemistry*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D000949:Histocompatibility Antigens Class II / Q000096:biosynthesis / Q000235:genetics; D016753:Interleukin-10 / Q000008:administration & dosage* / Q000235:genetics / Q000302:isolation & purification; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D000080385:Nanogels; D011092:Polyethylene Glycols / Q000737:chemistry*; D011094:Polyethyleneimine / Q000737:chemistry*; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000235:genetics / Q000302:isolation & purification; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000235:genetics
D003692:Delayed-Action Preparations; D003912:Dextrins; D004337:Drug Carriers; D000949:Histocompatibility Antigens Class II; D000080385:Nanogels; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; C413692:polyethylene glycol polyethyleneimine nanogel; D016753:Interleukin-10; D011092:Polyethylene Glycols; D011094:Polyethyleneimine