Involvement of TIP60 acetyltransferase in intracellular Salmonella replication.
BACKGROUND
Salmonella enterica is a facultative intracellular pathogen that replicates within a membrane-bound compartment termed Salmonella containing vacuole (SCV). The biogenesis of SCV requires Salmonella type III protein secretion/translocation system and their effector proteins which are translocated into host cells to exploit the vesicle trafficking pathways. SseF is one of these effectors required for SCV formation and Intracellular Salmonella replication through unknown mechanisms.


RESULTS
In an attempt to identify host proteins that interact with SseF, we conduct a yeast two-hybrid screening of human cell cDNA library using SseF as the bait. We identified that TIP60, an acetyltransferase, interacts with SseF. We showed that the TIP60 acetylation activity was increased in the presence of SseF, and TIP60 was upregulated upon Salmonella infection. In addition, TIP60 is required for efficient intracellular Salmonella replication in macrophages.


CONCLUSIONS
Taken together, our data suggest that Salmonella may use SseF to exploit the host TIP60 acetyltransferase activity to promote efficient Salmonella replication inside host cells.
Wang
Xueqin
X
Department of Molecular Virology, Shanghai Medical College of Fudan University, Shanghai 200032, PR China.
Li
Dongju
D
Qu
Di
D
Zhou
Daoguo
D
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002460:Cell Line; D004261:DNA Replication; D006367:HeLa Cells; D051548:Histone Acetyltransferases / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D000074443:Lysine Acetyltransferase 5; D008264:Macrophages / Q000201:enzymology / Q000382:microbiology; D051379:Mice; D011485:Protein Binding; D012480:Salmonella Infections / Q000201:enzymology* / Q000235:genetics / Q000382:microbiology*; D012486:Salmonella typhimurium / Q000235:genetics* / Q000378:metabolism
D001426:Bacterial Proteins; D051548:Histone Acetyltransferases; C098489:KAT5 protein, human; D000074443:Lysine Acetyltransferase 5
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