AICD Overexpression in Neuro 2A Cells Regulates Expression of PTCH1 and TRPC5.
Amyloid precursor protein (APP), implicated in Alzheimer's disease, is a transmembrane protein of undetermined function. APP is cleaved by gamma-secretase that releases the APP intracellular domain (AICD) in the cytoplasm. In vitro and in vivo studies have implicated the role of AICD in cell signaling and transcriptional regulation of Gsk3β, KAI1, BACE1, EGFR, and other proteins. In this study, by overexpressing AICD in mouse neuroblastoma cell lines, we have demonstrated the alteration in the expressions of two proteins, patched homolog 1 (PTCH1), a receptor for sonic hedgehog signaling, and transient receptor potential cation channel subfamily C member 5 (TRPC5), a component of receptor-activated nonselective calcium permeant cation channel. Our results indicate the possibility of regulation by AICD in developmental processes as well as in the maintenance of calcium homeostasis at the transcription level.
Raychaudhuri
Mithu
M
Structural Genomics Section, Saha Institute of Nuclear Physics, 1/AF Bidhan Nagar, WB, Kolkata 700 064, India.
Mukhopadhyay
Debashis
D
J Neurosci. 2000 Nov 1;20(21):7951-63
11050115
Nat Neurosci. 2003 Aug;6(8):837-45
12858178
Neuron. 2001 Mar;29(3):645-55
11301024
Ann N Y Acad Sci. 1993 May 28;679:1-21
8512177
Neuron. 2005 May 19;46(4):541-54
15944124
Curr Alzheimer Res. 2006 Apr;3(2):109-21
16611011
Ann N Y Acad Sci. 1993 Sep 24;695:158-64
8239276
J Cell Sci. 2004 Sep 1;117(Pt 19):4435-48
15331662
Am J Hum Genet. 2000 Nov;67(5):1047-54
11001584
FASEB J. 2003 Oct;17(13):1951-3
12923068
Cell. 1996 Jun 14;85(6):841-51
8681379
Nature. 1996 Nov 14;384(6605):129-34
8906787
Biochem Biophys Res Commun. 2006 Mar 3;341(1):1-5
16406235
J Cell Sci. 2000 Jun;113 ( Pt 11):1857-70
10806097
Science. 2001 Jul 6;293(5527):115-20
11441186
Traffic. 2002 Nov;3(11):763-70
12383342
J Alzheimers Dis. 2007 Jun;11(3):343-58
17851185
Cell. 2006 Oct 6;127(1):185-97
17018285
Perspect Dev Neurobiol. 1998;5(4):337-52
10533524
Proc Natl Acad Sci U S A. 2007 Jun 19;104(25):10613-8
17556541