Combined optical coherence tomography and electroretinography system for in vivo simultaneous morphological and functional imaging of the rodent retina.
A combined ultrahigh resolution optical coherence tomography (UHROCT) and a electroretinography (ERG) system is presented for simultaneous imaging of the retinal structure and physiological response to light stimulation in the rodent eye. The 1060-nm UHROCT system provides approximately 3x5 microm (axialxlateral) resolution in the rat retina and time resolution of 22 micros. A custom-designed light stimulator integrated into the UHROCT imaging probe provides light stimuli with user-selected color, duration, and intensity. The performance of the combined system is demonstrated in vivo in healthy rats, and in a rat model of drug-induced outer retinal degeneration. Experimental results show correlation between the observed structural and physiological changes in the healthy and degenerated retina.
Moayed
Alireza Akhlagh
AA
Hariri
Sepideh
S
Hyun
Chulho
C
Doran
Bruce
B
Kraft
Timothy W
TW
Boyd
Shelley
S
Bizheva
Kostadinka
K
D000818:Animals; D004596:Electroretinography / Q000295:instrumentation* / Q000662:veterinary*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005260:Female; D051381:Rats; D020318:Rats, Long-Evans; D012160:Retina / Q000166:cytology* / Q000502:physiology*; D042261:Retinoscopes / Q000662:veterinary*; D018511:Systems Integration; D041623:Tomography, Optical Coherence / Q000295:instrumentation* / Q000662:veterinary*