Single laser source for multimodal coherent anti-Stokes Raman scattering microscopy.
Short laser pulse technology has significantly contributed to biomedical research, especially via nonlinear optical microscopy. Coherent anti-Stokes Raman scattering (CARS) microscopy is a label-free, chemical-selective method that is growing in importance as improved methods and light sources develop. Here we discuss different approaches to laser source development for CARS microscopy and highlight the advantages of a multimodal CARS microscope, illustrated by selected applications in biomedical research.
Pegoraro
Adrian F
AF
Department of Physics, Queen's University, Kingston, Ontario K7L 3N6, Canada. adrian.pegoraro@nrc-cnrc.gc.ca
Slepkov
Aaron D
AD
Ridsdale
Andrew
A
Pezacki
John Paul
JP
Stolow
Albert
A
D000818:Animals; D002460:Cell Line; D002477:Cells / Q000648:ultrastructure*; D006801:Humans; D007089:Image Enhancement; D007834:Lasers; D051379:Mice; D008853:Microscopy / Q000295:instrumentation / Q000379:methods*; D013059:Spectrum Analysis, Raman / Q000295:instrumentation / Q000379:methods*