title: Pyramidal neurons are generated from oligodendroglial progenitor cells in adult piriform cortex.
abstract: Previous studies have shown that oligodendroglial progenitor cells (OPCs) can give rise to neurons in vitro and in perinatal cerebral cortex in vivo. We now report that OPCs in adult murine piriform cortex express low levels of doublecortin, a marker for migratory and immature neurons. Additionally, these OPCs express Sox2, a neural stem cell marker, and Pax6, a transcription factor characteristic of progenitors for cortical glutamatergic neurons. Genetic fate-mapping by means of an inducible Cre-LoxP recombination system proved that these OPCs differentiate into pyramidal glutamatergic neurons in piriform cortex. Several lines of evidence indicated that these newly formed neurons became functionally integrated into the cortical neuronal network. Our data suggest that NG2(+)/PDGFRα(+) proteolipid protein promoter-expressing progenitors generate pyramidal glutamatergic neurons within normal adult piriform cortex.
authors.affiliation: Institute for Pediatric Regenerative Medicine, University of California, Davis School of Medicine, Sacramento, California 95817, USA.
authors.forename: Fuzheng
authors.initials: F
authors.lastname: Guo
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Maeda
authors.forename: Joyce
authors.initials: J
authors.lastname: Ma
authors.forename: Jie
authors.initials: J
authors.lastname: Xu
authors.forename: Makoto
authors.initials: M
authors.lastname: Horiuchi
authors.forename: Laird
authors.initials: L
authors.lastname: Miers
authors.forename: Flora
authors.initials: F
authors.lastname: Vaccarino
authors.forename: David
authors.initials: D
authors.lastname: Pleasure
mesh_terms: D053687:Adult Stem Cells / Q000187:drug effects / Q000502:physiology*; D000818:Animals; D000941:Antigens / Q000378:metabolism; D018931:Antineoplastic Agents, Hormonal / Q000494:pharmacology; D001973:Bromodeoxyuridine / Q000378:metabolism; D002452:Cell Count / Q000379:methods; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D002540:Cerebral Cortex / Q000166:cytology*; D000089622:Doublecortin Domain Proteins; D004334:Drug Administration Schedule; D005136:Eye Proteins / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000235:genetics; D018398:Homeodomain Proteins / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D018991:Myelin Proteolipid Protein / Q000235:genetics; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009474:Neurons / Q000502:physiology; D009479:Neuropeptides / Q000378:metabolism; D009836:Oligodendroglia / Q000502:physiology*; D000071841:PAX6 Transcription Factor; D051761:Paired Box Transcription Factors / Q000378:metabolism; D011509:Proteoglycans / Q000378:metabolism; D017966:Pyramidal Cells / Q000502:physiology*; D020796:Receptor, Platelet-Derived Growth Factor alpha / Q000378:metabolism; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism; D012097:Repressor Proteins / Q000378:metabolism; D055748:SOXB1 Transcription Factors / Q000378:metabolism; D013629:Tamoxifen / Q000494:pharmacology; D013997:Time Factors; D000099216:Chondroitin Sulfate Proteoglycan 4
chemical_list: D000941:Antigens; D018931:Antineoplastic Agents, Hormonal; D001973:Bromodeoxyuridine; D000089622:Doublecortin Domain Proteins; D005136:Eye Proteins; D049452:Green Fluorescent Proteins; D018398:Homeodomain Proteins; D008869:Microtubule-Associated Proteins; D018991:Myelin Proteolipid Protein; D009419:Nerve Tissue Proteins; D009479:Neuropeptides; D000071841:PAX6 Transcription Factor; D051761:Paired Box Transcription Factors; D011509:Proteoglycans; D020796:Receptor, Platelet-Derived Growth Factor alpha; D016194:Receptors, N-Methyl-D-Aspartate; D012097:Repressor Proteins; D055748:SOXB1 Transcription Factors; D013629:Tamoxifen; D000099216:Chondroitin Sulfate Proteoglycan 4; C409466:NR1 NMDA receptor; C000605732:Pax6 protein, mouse; C047516:Plp1 protein, mouse; C527783:Sox2 protein, mouse; C485184:enhanced green fluorescent protein
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