Membrane perturbation induced by papiliocin peptide, derived from Papilio xuthus, in Candida albicans.
Previously, papiliocin was isolated from the swallowtail butterfly Papilio xuthus and its antimicrobial activity was suggested. In this study, the antifungal mechanism of papiliocin was investigated against Candida albicans. Confocal laser scanning microscopy (CLSM) and 1,6-diphenyl-1,3,5-hexatriene (DPH) fluorescence analysis indicated that papiliocin disturbed the fungal plasma membrane. Moreover, the assessment of the release of FITC-dextran (FD) from liposome further demonstrated that the antifungal mechanism of papiliocin could have originated from the pore-forming action and that the radius of the pores was presumed to be anywhere from 2.3 nm and 3.3 nm.
Lee
Juneyoung
J
School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University, Daegu, Korea.
Hwang
Jae-Sam
JS
Hwang
Bomi
B
Kim
Jin-Kyoung
JK
Kim
Seong Ryul
SR
Kim
Yangmee
Y
Lee
Dong Gun
DG
D000818:Animals; D000935:Antifungal Agents / Q000378:metabolism / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides / Q000378:metabolism / Q000494:pharmacology*; D002080:Butterflies / Q000737:chemistry* / Q000378:metabolism; D002176:Candida albicans / Q000737:chemistry / Q000187:drug effects*; D002462:Cell Membrane / Q000737:chemistry / Q000187:drug effects*
D000935:Antifungal Agents; D023181:Antimicrobial Cationic Peptides; C551727:papiliocin protein, Papilio xuthus