Localization microscopy reveals expression-dependent parameters of chromatin nanostructure.
A combined approach of 2D high-resolution localization light microscopy and statistical methods is presented to infer structural features and density fluctuations at the nuclear nanoscale. Hallmarks of nuclear nanostructure are found on the scale below 100 nm for both human fibroblast and HeLa cells. Mechanical measures were extracted as a quantitative tool from the histone density fluctuations inside the cell to obtain structural fluctuations on the scale of several micrometers. Results show that different mechanisms of expression of the same nuclear protein type lead to significantly different patterns on the nanoscale and to pronounced differences in the detected compressibility of chromatin. The observed fluctuations, including the experimental evidence for dynamic looping, are consistent with a recently proposed chromatin model.
Bohn
Manfred
M
Institute for Theoretical Physics, Heidelberg University, Heidelberg, Germany. bohn@tphys.uni-heidelberg.de
Diesinger
Philipp
P
Kaufmann
Rainer
R
Weiland
Yanina
Y
Müller
Patrick
P
Gunkel
Manuel
M
von Ketteler
Alexa
A
Lemmer
Paul
P
Hausmann
Michael
M
Heermann
Dieter W
DW
Cremer
Christoph
C
D002467:Cell Nucleus / Q000378:metabolism; D002843:Chromatin / Q000737:chemistry* / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000378:metabolism; D005786:Gene Expression Regulation; D006367:HeLa Cells; D006657:Histones / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008027:Light; D008853:Microscopy / Q000379:methods* / Q000706:statistics & numerical data; D049329:Nanostructures; D021381:Protein Transport
D002843:Chromatin; D005456:Fluorescent Dyes; D006657:Histones
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