title: Relationship of hemoglobin concentration to packed cell volume in avian blood samples.
abstract: To determine whether a proportional relationship exists between hemoglobin (Hgb) concentration and pack cell volume (PCV) in avian blood samples, Hgb concentration measured by a point-of-care portable hemoglobinometer and corresponding PCV were determined in blood samples collected from 128 birds comprising 13 avian orders. For all birds evaluated and pooled across orders, a significant and consistent relationship was found between Hgb concentration and PCV, expressed as Hgb = 0.304 x PCV + 0.461. To determine whether the relationship of PCV and Hgb differs, 9 avian orders with n > or = 8 samples per order were analyzed by linear regression. Individual slopes for the 9 orders did not differ significantly (P = .44), indicating that a single slope can be used to model the avian Hgb to PCV relationship for these taxonomic orders. A single intercept can also be used, with the exception of order Phoenicopteriformes, which was the only intercept that was significantly different from 0 (P = .01). These results indicate that a simplified relationship of Hgb (g/dL) = 0.30 x PCV provides a reasonable estimate of Hgb concentration from the PCV of birds from the orders Anseriformes, Columbiformes, Falconiformes, Galliformes, Passeriformes, Psittaciformes, Sphenisciformes, and Strigiformes, but a separate relationship of Hgb = 0.217 x PCV + 6.69 might be warranted for the order Phoenicopteriformes.
authors.affiliation: Department of Veterinary Pathobiology, College of Veterinary Medicine, Center for Veterinary Health Sciences , Oklahoma State University, Stillwater, OK 74078, USA.
authors.forename: Karen E
authors.initials: KE
authors.lastname: Velguth
authors.forename: Mark E
authors.initials: ME
authors.lastname: Payton
authors.forename: John P
authors.initials: JP
authors.lastname: Hoover
mesh_terms: D000818:Animals; D001717:Birds / Q000097:blood* / Q000145:classification / Q000235:genetics; D006400:Hematocrit / Q000662:veterinary*; D006452:Hemoglobinometry / Q000662:veterinary; D006454:Hemoglobins / Q000032:analysis*; D013045:Species Specificity
chemical_list: D006454:Hemoglobins