Breeding an amylolytic yeast strain for alcoholic beverage production.
A starch-utilizing, yeast-like fusant was successfully created from fused protoplasts of Schizosaccharomyces pombe and Monascus anka, and the feasibility of using this fusant as a new strain for alcoholic beverage development was reported. The new fusant utilized various carbon sources more efficiently than its parent cells did. Rice koji prepared separately by cultivating the fusant and its parental strains on rice was compared to explore the effect of yeast strain on the production of α-amylase, glucoamylase, and acid protease that are crucial in wine making using cereal grains. It was found that the fusant produced greater levels of the above-mentioned enzymes than its parental strain does. Consequently, the usage of this fusant in the alcoholic fermentation of polished rice was found to reduce approximately 50% consumption of added glucoamylase than when its parental strain was used. Besides, at the end of fermentation, the fusant yeast resulted in a mash with distribution of flavor components very different from that produced by its parental strains. Thus, the fusant can be used as a new yeast strain for creating novel alcoholic beverages.
Cheng
Ming-Chung
MC
Department of Food Science, National Pingtung University of Science and Technology, Neipu, Pingtung, Taiwan, Republic of China. pea007@ms15.hinet.net
Chang
Rei-Chu
RC
Dent
Der-Feng
DF
Hsieh
Pao-Chuan
PC
D000222:Adaptation, Physiological / Q000187:drug effects; D000434:Alcoholic Beverages; D000688:Amylose / Q000378:metabolism*; D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D002244:Carbon / Q000494:pharmacology; D002459:Cell Fusion; D003470:Culture Media / Q000494:pharmacology; D057075:Enzyme Assays; D000431:Ethanol / Q000494:pharmacology; D005285:Fermentation / Q000187:drug effects; D032741:Monascus / Q000166:cytology / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development*; D009584:Nitrogen / Q000494:pharmacology; D011523:Protoplasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D012568:Schizosaccharomyces / Q000166:cytology / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development*; D013649:Taste
D003470:Culture Media; D000431:Ethanol; D002244:Carbon; D000688:Amylose; D009584:Nitrogen