Microfluidic monitoring of Pseudomonas aeruginosa chemotaxis under the continuous chemical gradient.
This study presents a microfluidic approach for the rapid analysis of bacterial chemotaxis in response to chemical gradients. The diffusional mixing of laminar flow continuously generates a stable chemical gradient in a microfluidic device. For the proof of concept, we have investigated the effects of the attractant peptone and repellent trichloroethylene (TCE) on chemotactic responses of wild type Pseudomonas aeruginosa PAO1 and chemotactic mutant PC4. The microfluidic method clearly demonstrates that P. aeruginosa PAO1 is attracted to peptone and repelled from TCE, whereas PC4 shows non-chemotactic behavior. In addition, the analysis of PAO1 chemotaxis on 20 amino acids revealed the effective concentration range of each amino acid as a chemoeffector. Not only does the microfluidic approach facilitate the quantitative information of chemotaxis, which gives an insight into understanding the mechanism of P. aeruginosa motility, but it also provides a useful tool for the rapid monitoring of bacterial chemotaxis in a reproducible experimental manner.
Jeong
Heon-Ho
HH
Department of Chemical Engineering, Chungnam National University, Daejeon 305-764, Republic of Korea.
Lee
Sang-Ho
SH
Kim
Jong-Min
JM
Kim
Hye-Eun
HE
Kim
Yun-Gon
YG
Yoo
Jung Yul
JY
Chang
Woo-Suk
WS
Lee
Chang-Soo
CS
D018929:Cell Culture Techniques / Q000295:instrumentation*; D002630:Chemotactic Factors / Q000008:administration & dosage*; D002633:Chemotaxis / Q000187:drug effects / Q000502:physiology*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D017022:Flow Injection Analysis / Q000295:instrumentation*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D011550:Pseudomonas aeruginosa / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*
D002630:Chemotactic Factors