title: The mitosis-to-interphase transition is coordinated by cross talk between the SIN and MOR pathways in Schizosaccharomyces pombe.
abstract: The mechanisms that regulate cytoskeletal remodeling during the transition between mitosis and interphase are poorly understood. In fission yeast the MOR pathway promotes actin polarization to cell tips in interphase, whereas the SIN signaling pathway drives actomyosin ring assembly and cytokinesis. We show that the SIN inhibits MOR signaling in mitosis by interfering with Nak1 kinase-mediated activation of the most downstream MOR component, the NDR family kinase Orb6. Inactivation of the MOR may be a key function of the SIN because attenuation of MOR signaling rescued the cytokinetic defects of SIN mutants and allowed weak SIN signaling to trigger ectopic cytokinesis. Furthermore, failure to inhibit the MOR is toxic when the cell division apparatus is compromised. Together, our results reveal a mutually antagonistic relationship between the SIN and MOR pathways, which is important for completion of cytokinesis and coordination of cytoskeletal remodeling at the mitosis-to-interphase transition.
authors.affiliation: Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, MA 01605, USA.
authors.forename: Samriddha
authors.initials: S
authors.lastname: Ray
authors.forename: Kazunori
authors.initials: K
authors.lastname: Kume
authors.forename: Sneha
authors.initials: S
authors.lastname: Gupta
authors.forename: Wanzhong
authors.initials: W
authors.lastname: Ge
authors.forename: Mohan
authors.initials: M
authors.lastname: Balasubramanian
authors.forename: Dai
authors.initials: D
authors.lastname: Hirata
authors.forename: Dannel
authors.initials: D
authors.lastname: McCollum
mesh_terms: D000199:Actins / Q000235:genetics / Q000378:metabolism; D002455:Cell Division; D048749:Cytokinesis / Q000235:genetics / Q000502:physiology; D049452:Green Fluorescent Proteins / Q000378:metabolism; D007399:Interphase / Q000235:genetics; D008938:Mitosis; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012568:Schizosaccharomyces / Q000166:cytology / Q000235:genetics / Q000378:metabolism; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D015398:Signal Transduction / Q000235:genetics
chemical_list: D000199:Actins; D011993:Recombinant Fusion Proteins; D029702:Schizosaccharomyces pombe Proteins; D049452:Green Fluorescent Proteins
references.citation: Oncogene. 2005 Mar 17;24(12):2076-86
references.pmid: 15688006
references.citation: J Cell Biol. 1999 Aug 23;146(4):777-90
references.pmid: 10459013
references.citation: J Biol Chem. 2008 Feb 29;283(9):5496-509
references.pmid: 18158288
references.citation: Curr Biol. 2001 Jun 26;11(12):931-40
references.pmid: 11448769
references.citation: Nat Cell Biol. 2000 Nov;2(11):855-8
references.pmid: 11056543
references.citation: Dev Cell. 2009 Apr;16(4):551-62
references.pmid: 19386264
references.citation: Biochem Soc Trans. 2008 Jun;36(Pt 3):411-5
references.pmid: 18481970
references.citation: J Cell Biol. 2008 Dec 15;183(6):979-88
references.pmid: 19075108
references.citation: Eur J Cell Biol. 2005 Dec;84(12):915-26
references.pmid: 16325501
references.citation: Nature. 2006 Sep 14;443(7108):210-3
references.pmid: 16906135
references.citation: Cell. 1995 Aug 11;82(3):435-44
references.pmid: 7634333
references.citation: EMBO J. 1993 Jul;12(7):2697-704
references.pmid: 8334988
references.citation: Curr Biol. 2009 Aug 11;19(15):1314-9
references.pmid: 19646873
references.citation: Yeast. 2006 Feb;23(3):173-83
references.pmid: 16498704
references.citation: J Cell Biol. 2004 Mar 29;164(7):1009-20
references.pmid: 15037601
references.citation: J Cell Biol. 1994 Jun;125(6):1289-301
references.pmid: 8207058
references.citation: Dev Suppl. 1993;:289-99
references.pmid: 8049484
references.citation: Biosci Biotechnol Biochem. 2007 Feb;71(2):615-7
references.pmid: 17317928
references.citation: Methods Enzymol. 1991;194:795-823
references.pmid: 2005825
references.citation: J Cell Biol. 2009 Sep 7;186(5):739-53
references.pmid: 19736319
references.citation: Biochim Biophys Acta. 2008 Jan;1784(1):3-15
references.pmid: 17881309
references.citation: Genetics. 1998 Jul;149(3):1265-75
references.pmid: 9649519
references.citation: Curr Biol. 2005 Aug 9;15(15):1384-9
references.pmid: 16085490
references.citation: Nat Rev Mol Cell Biol. 2006 Apr;7(4):253-64
references.pmid: 16607288
references.citation: Gene. 1998 Oct 9;221(1):59-68
references.pmid: 9852950
references.citation: Cancer Res. 2008 Apr 15;68(8):2789-94
references.pmid: 18413746
references.citation: J Cell Sci. 2003 Nov 1;116(Pt 21):4263-75
references.pmid: 14514882
references.citation: Clin Cancer Res. 2005 Feb 15;11(4):1380-5
references.pmid: 15746036
references.citation: Curr Biol. 2008 Dec 9;18(23):1889-95
references.pmid: 19062280
references.citation: Curr Biol. 2007 Apr 17;17(8):700-5
references.pmid: 17379520
references.citation: Mol Cell Biol. 2005 Sep;25(18):8259-72
references.pmid: 16135814
references.citation: Mol Carcinog. 2007 Oct;46(10):865-71
references.pmid: 17538946
references.citation: Cancer Res. 2005 Aug 1;65(15):6568-75
references.pmid: 16061636
references.citation: J Cell Sci. 2005 Mar 1;118(Pt 5):1033-44
references.pmid: 15731009
references.citation: Genes Genet Syst. 2004 Oct;79(5):283-91
references.pmid: 15599058
references.citation: J Biol Chem. 2003 Jan 10;278(2):991-7
references.pmid: 12427731
references.citation: Genes Dev. 2008 Nov 15;22(22):3082-8
references.pmid: 19056889
references.citation: Trends Cell Biol. 2009 Sep;19(9):447-54
references.pmid: 19713114
references.citation: J Cell Sci. 2000 Dec;113 Pt 23:4157-63
references.pmid: 11069761
references.citation: Methods Enzymol. 1997;283:494-506
references.pmid: 9251043
references.citation: J Bacteriol. 1979 Jan;137(1):440-6
references.pmid: 762020
references.citation: EMBO J. 2002 Sep 16;21(18):4863-74
references.pmid: 12234926
references.citation: Genes Dev. 1997 Jun 15;11(12):1519-34
references.pmid: 9203579
references.citation: Development. 2001 Jul;128(14):2793-802
references.pmid: 11526084
references.citation: EMBO J. 2005 Sep 7;24(17):3012-25
references.pmid: 16096637
references.citation: Proc Natl Acad Sci U S A. 1998 Jun 23;95(13):7526-31
references.pmid: 9636183
references.citation: J Cell Sci. 2004 Aug 1;117(Pt 17):3897-910
references.pmid: 15265986
references.citation: J Cell Sci. 2000 Apr;113 ( Pt 7):1223-30
references.pmid: 10704373
references.citation: EMBO J. 2000 Apr 17;19(8):1803-15
references.pmid: 10775265
references.citation: FEBS Lett. 2003 Jul 3;546(1):73-80
references.pmid: 12829239
references.citation: Genes Dev. 2008 Nov 15;22(22):3205-16
references.pmid: 19056897