title: Thirty-seven transcription factor genes differentially respond to a harpin protein and affect resistance to the green peach aphid in Arabidopsis.
abstract: The harpin protein HrpN Ea induces Arabidopsis resistance to the green peach aphid by activating the ethylene signalling pathway and by recruiting EIN2, an essential regulator of ethylene signalling, for a defence response in the plant. We investigated 37 ethylene-inducible Arabidopsis transcription factor genes for their effects on the activation of ethylene signalling and insect defence. Twenty-eight of the 37 genes responded to both ethylene and HrpN Ea, and showed either increased or inhibited transcription, while 18 genes showed increased transcription not only by ethylene but also by HrpN Ea. In response to HrpN Ea, transcription levels of 22 genes increased, with AtMYB44 being the most inducible, six genes had decreased transcript levels, and nine remained unchanged. When Arabidopsis mutants previously generated by mutagenicity at the 37 genes were surveyed, 24 mutants were similar to the wild type plant while four mutants were more resistant and nine mutants were more susceptible than wild type to aphid infestation. Aphid-susceptible mutants showed a greater susceptibility for atmyb15, atmyb38 and atmyb44, which were generated previously by T-DNA insertion into the exon region of AtMYB15 and the promoter regions of AtMYB38 and AtMYB44. The atmyb44 mutant was the most susceptible to aphid infestation and most compromised in induced resistance. Resistance accompanied the expression of PDF1.2, an ethylene signalling marker gene that requires EIN2 for transcription in wild type but not in atmyb15, atmyb38, and atmyb44, suggesting a disruption of ethylene signalling in the mutants. However, only atmyb44 incurred an abrogation in induced EIN2 expression, suggesting a close relationship between AtMYB44 and EIN2.
authors.affiliation: State Ministry of Agriculture Key Laboratory of Monitoring and Management of Crop Pathogens and Insect Pests, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Ruoxue
authors.initials: R
authors.lastname: Liu
authors.forename: Beibei
authors.initials: B
authors.lastname: Lü
authors.forename: Xiaomeng
authors.initials: X
authors.lastname: Wang
authors.forename: Chunling
authors.initials: C
authors.lastname: Zhang
authors.forename: Shuping
authors.initials: S
authors.lastname: Zhang
authors.forename: Jun
authors.initials: J
authors.lastname: Qian
authors.forename: Lei
authors.initials: L
authors.lastname: Chen
authors.forename: Haojie
authors.initials: H
authors.lastname: Shi
authors.forename: Hansong
authors.initials: H
authors.lastname: Dong
mesh_terms: D000818:Animals; D001042:Aphids / Q000502:physiology*; D017360:Arabidopsis / Q000469:parasitology / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000378:metabolism; D001425:Bacterial Outer Membrane Proteins / Q000502:physiology*; D023082:Defensins / Q000378:metabolism; D005030:Ethylenes / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D006790:Host-Parasite Interactions; D011956:Receptors, Cell Surface / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D001425:Bacterial Outer Membrane Proteins; D023082:Defensins; D005030:Ethylenes; D011956:Receptors, Cell Surface; D014157:Transcription Factors; C119963:EIN2 protein, Arabidopsis; C529221:At5g67300 protein, Arabidopsis; C487542:At5g44420 protein, Arabidopsis; C075462:harpin protein, Erwinia amylovora; C036216:ethylene
references.citation: Curr Opin Plant Biol. 2001 Oct;4(5):447-56
references.pmid: 11597504
references.citation: Phytopathology. 2008 Jul;98(7):781-91
references.pmid: 18943254
references.citation: Plant J. 1999 Oct;20(2):207-15
references.pmid: 10571880
references.citation: Science. 1992 Jul 3;257(5066):85-8
references.pmid: 1621099
references.citation: Phytopathology. 2004 Oct;94(10):1048-55
references.pmid: 18943792
references.citation: Plant Physiol. 2004 Nov;136(3):3628-38
references.pmid: 15516507
references.citation: Phytopathology. 2008 Jul;98(7):792-802
references.pmid: 18943255
references.citation: J Biol Chem. 2006 Dec 8;281(49):37636-45
references.pmid: 17015446
references.citation: Plant Cell. 2007 Aug;19(8):2440-53
references.pmid: 17675404
references.citation: Plant Physiol. 2001 Feb;125(2):1074-85
references.pmid: 11161062
references.citation: Plant Cell Rep. 2007 Jul;26(7):1053-63
references.pmid: 17297615
references.citation: Plant Cell. 2000 Jul;12(7):1117-26
references.pmid: 10899978
references.citation: Curr Opin Plant Biol. 2006 Oct;9(5):544-9
references.pmid: 16877030
references.citation: Plant Mol Biol. 2006 Jan;60(1):107-24
references.pmid: 16463103
references.citation: Phytopathology. 2006 Oct;96(10):1052-9
references.pmid: 18943492
references.citation: Plant Cell. 1990 Jun;2(6):513-23
references.pmid: 2152173
references.citation: Plant Cell. 1998 Dec;10(12):2103-13
references.pmid: 9836748
references.citation: Plant Cell. 2002;14 Suppl:S131-51
references.pmid: 12045274
references.citation: Plant Physiol. 2008 Feb;146(2):623-35
references.pmid: 18162593
references.citation: Plant Physiol. 2002 Jun;129(2):838-53
references.pmid: 12068123
references.citation: Plant Physiol. 2004 Jun;135(2):773-82
references.pmid: 15208423
references.citation: Plant Cell. 2009 Aug;21(8):2527-40
references.pmid: 19717619
references.citation: Plant Cell Physiol. 2001 Apr;42(4):446-9
references.pmid: 11333317
references.citation: Science. 2003 Aug 1;301(5633):653-7
references.pmid: 12893945
references.citation: Science. 1995 Dec 15;270(5243):1809-11
references.pmid: 8525372
references.citation: New Phytol. 2010 Mar;185(4):1062-73
references.pmid: 20085621
references.citation: Plant J. 1998 Oct;16(2):263-76
references.pmid: 9839469
references.citation: Phytopathology. 2010 Jun;100(6):573-81
references.pmid: 20465413
references.citation: Planta. 2005 Jun;221(3):313-27
references.pmid: 15599761
references.citation: J Biol Chem. 1995 May 26;270(21):12526-30
references.pmid: 7759498
references.citation: Plant Mol Biol. 1998 Jul;37(5):819-27
references.pmid: 9678577
references.citation: Methods. 2001 Dec;25(4):402-8
references.pmid: 11846609
references.citation: J Biosci. 2006 Dec;31(5):617-27
references.pmid: 17301500
references.citation: Proc Natl Acad Sci U S A. 2009 Oct 27;106(43):18414-9
references.pmid: 19820165
references.citation: Biochem Biophys Res Commun. 2007 Oct 5;361(4):1048-53
references.pmid: 17686455
references.citation: Mol Cells. 2010 Jan;29(1):71-6
references.pmid: 20016937
references.citation: Science. 1999 Jun 25;284(5423):2148-52
references.pmid: 10381874
references.citation: Curr Opin Plant Biol. 2004 Feb;7(1):40-9
references.pmid: 14732440
references.citation: J Biosci. 2007 Sep;32(6):1119-31
references.pmid: 17954972
references.citation: J Integr Plant Biol. 2009 Feb;51(2):167-74
references.pmid: 19200155
references.citation: Proc Natl Acad Sci U S A. 1997 May 13;94(10):5473-7
references.pmid: 11038546
references.citation: Plant Mol Biol. 2005 Dec;59(6):853-68
references.pmid: 16307362