Apoptosis in diabetic fibrovascular membranes after panretinal photocoagulation.
A more complete understanding of the role of apoptosis in the regression of diabetic neovasculature following laser panretinal photocoagulation (PRP) will both elucidate the treatment's therapeutic mechanism and potentially lead to novel treatments for neovascularization associated with proliferative diabetic retinopathy that target apoptotic pathways. Pars plana vitrectomy with fibrovascular membrane delamination was performed on five patients with proliferative diabetic retinopathy, with four having received previous PRP treatment and one no previous laser treatment. Using in situ terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling, propidium iodide and hematoxylin-eosin staining, apoptotic cells were identified in the excised membranes. The authors found evidence of cells undergoing apoptosis in all of the excised membranes, with increasing amounts of preoperative PRP associated with an increased number of apoptotic cells per millimeter of membrane. The preliminary data suggest that the decrease in ambient mitogen, initiated by PRP treatment, activates apoptosis in diabetic fibrovascular membranes.
Tran
Anh T
AT
University of Massachusetts Medical School, Department of Ophthalmology, Boston, Massachusetts, USA.
Bula
Deisy V
DV
Kovacs
Kyle D
KD
Savageau
Judy
J
Arroyo
Jorge G
JG
D000328:Adult; D017209:Apoptosis; D003930:Diabetic Retinopathy / Q000601:surgery*; D004730:Endothelium, Vascular / Q000473:pathology* / Q000503:physiopathology; D019773:Epiretinal Membrane / Q000209:etiology / Q000473:pathology / Q000503:physiopathology*; D005260:Female; D006801:Humans; D017075:Laser Coagulation / Q000009:adverse effects*; D008297:Male; D008875:Middle Aged; D012160:Retina / Q000601:surgery*; D014821:Vitrectomy / Q000379:methods