Autoassembly protein arrays for analyzing antibody cross-reactivity.
We report an autoassembly protein array capable of rapidly screening for aberrant antibody-antigen binding events. Our technique combines magnetic nanoparticle technology with proximity-based, magnetically responsive nanosensors for rapid (under 15 min) and high-density screening of antibody cross-reactivity at sensitivities down to 50 fM in a homogeneous assay. This method will enable the identification of the precise cause of aberrant or cross-reactive binding events in an easy-to-use, rapid, and high-throughput manner.
Gaster
Richard S
RS
Department of Bioengineering, School of Medicine, Stanford University, Stanford, California 94305, United States.
Hall
Drew A
DA
Wang
Shan X
SX
D000906:Antibodies / Q000032:analysis* / Q000276:immunology*; D000937:Antigen-Antibody Reactions; D003429:Cross Reactions / Q000276:immunology*; D057166:High-Throughput Screening Assays; D007118:Immunoassay; D008280:Magnetics; D036103:Nanotechnology; D040081:Protein Array Analysis
D000906:Antibodies
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