Brain expression of Cre recombinase driven by pancreas-specific promoters.
Cre-loxP technology enables specific examination of the function and development of individual nuclei in the complex brain network. However, for most brain regions, the utilization of this technique has been hindered by the lack of mouse lines with Cre expression restricted to these regions. Here, we identified brain expressions of three transgenic Cre lines previously thought to be pancreas-specific. Cre expression driven by the rat-insulin promoter (Rip-Cre) was found mainly in the arcuate nucleus, and to a lesser degree in other hypothalamic regions. Cre expression driven by the neurogenin 3 promoter (Ngn3-Cre mice) was found in the ventromedial hypothalamus. Cre expression driven by the pancreas-duodenum homeobox 1 promoter (Pdx1-Cre) was found in several hypothalamic nuclei, the dorsal raphe and inferior olivary nuclei. Interestingly, Pdx1-Cre mediated deletion of vesicular GABA transporter led to postnatal growth retardation while Ngn3-Cre mediated deletion had no effects, suggesting a role for Pdx1-Cre neurons, but not pancreas, in the regulation of postnatal growth. These results demonstrate the potential for these Cre lines to study the function and development of brain neurons.
Song
Junghun
J
Xu
Yuanzhong
Y
Hu
Xiaoxia
X
Choi
Brian
B
Tong
Qingchun
Q
D000818:Animals; D001921:Brain / Q000196:embryology / Q000378:metabolism*; D004622:Embryo, Mammalian; D005260:Female; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D019426:Integrases / Q000235:genetics* / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009928:Organ Specificity / Q000235:genetics; D010179:Pancreas / Q000378:metabolism*; D011247:Pregnancy; D011401:Promoter Regions, Genetic / Q000502:physiology*; D051381:Rats; D019076:Transgenes / Q000235:genetics
C045073:Cre recombinase; D019426:Integrases
Mol Cell Neurosci. 1996;8(4):221-41
9000438
Nat Neurosci. 2010 Jan;13(1):133-40
20023653
J Clin Invest. 2004 Oct;114(7):917-27
15467830
Biochem Biophys Res Commun. 2008 Nov 28;376(4):694-9
18809381
Biol Psychiatry. 2003 Dec 1;54(11):1125-33
14643079
PLoS Genet. 2009 Jul;5(7):e1000575
19629168
Curr Biol. 1998 Dec 3;8(24):1323-6
9843687
Cell Metab. 2006 Oct;4(4):291-302
17011502
Science. 2005 Aug 5;309(5736):943-7
16081739
BMC Dev Biol. 2007 Jan 12;7:4
17222338
Pharmacol Ther. 2007 Mar;113(3):619-34
17289150
Biochim Biophys Acta. 2007 Sep;1773(9):1455-61
17599554
Cell Metab. 2009 Apr;9(4):362-74
19356717
Diabetes. 2004 Dec;53(12):3131-41
15561943
J Clin Invest. 2004 Oct;114(7):908-16
15467829
J Biol Chem. 2000 Jun 23;275(25):19106-14
10751390
J Clin Invest. 2007 Oct;117(10):2860-8
17909627
Cell Metab. 2005 Jan;1(1):63-72
16054045
Nature. 2005 Apr 21;434(7036):1026-31
15846348
Nat Med. 2002 Dec;8(12):1376-82
12426561
Mech Dev. 2003 Jan;120(1):35-43
12490294
Nat Neurosci. 2008 Sep;11(9):998-1000
19160495
Obesity (Silver Spring). 2006 Aug;14 Suppl 5:222S-227S
17021371
Development. 2002 May;129(10):2447-57
11973276
Cell Metab. 2007 May;5(5):383-93
17488640
J Cell Mol Med. 2009 Nov-Dec;13(11-12):4403-8
19040414
Gene. 2001 Sep 5;275(1):47-55
11574151
Genesis. 2000 Nov-Dec;28(3-4):147-55
11105057
Methods Mol Biol. 2009;493:65-85
18839342
Cell Mol Neurobiol. 2003 Feb;23(1):1-25
12701881
J Clin Invest. 2005 Apr;115(4):940-50
15841180
Brain Res. 2007 Mar 2;1135(1):31-40
17196948
Endocrinology. 1999 Aug;140(8):3857-60
10433248
Endocrine. 2002 Dec;19(3):267-78
12624426
J Neurochem. 2007 May;101(3):757-70
17448147
Cerebellum. 2006;5(1):15-22
16527759