[Rapid biochemical method for the detection of paralytical shellfish poisoning toxins in shellfish from seafood market].
A rapid biochemical method was discussed in order to detect low concentration of paralytic shellfish poisoning (PSP) in sea food. The mice were injected with PSP extract of bivalves (1 and 0.2 microg/kg respectively, as STX equivalents) purchased from seafood market. ACh, AChE, NO and NOS in blood were studied at 15, 60, 120 min respectively. The results showed that at low dose (0.2 microg/kg) and 15 min, only the contents of ACh changed significantly compared with control group (p < 0.05), which was (141.2 +/- 14.8) microg/mg, while the contents of NO and the activities of NOS changed until 120 min, compared to control group (p < 0.05) ,which were (68.7 +/- 3.8) micromol/g and (40.1 +/- 4.9) U/mg respectively. At high dose the contents of ACh changed at all three time point. It can be suggested that the contents of ACh is the only one of four indexes which can response to PSP at low dose in an early stage (15 min) and may be selected as a biochemical index for rapid detection of PSP.
Liu
Yuan-yuan
YY
School of Environmental Science and Engineering, Shanghai Jiaotong University, Shanghai 200240, China. yy_liu@sjtu.edu.cn
Cheng
Jin-ping
JP
Gao
Li-li
LL
Wang
Jin-hui
JH
Song
Yu-ling
YL
Chen
Xue
X
Wang
Wen-hua
WH
D000109:Acetylcholine / Q000097:blood*; D000818:Animals; D001681:Biological Assay / Q000379:methods; D049872:Bivalvia / Q000737:chemistry; D005506:Food Contamination / Q000032:analysis*; D005517:Foodborne Diseases / Q000517:prevention & control; D051379:Mice; D008813:Mice, Inbred ICR; D008978:Mollusk Venoms / Q000032:analysis*; D012680:Sensitivity and Specificity; D012758:Shellfish / Q000032:analysis*; D057096:Shellfish Poisoning / Q000097:blood* / Q000517:prevention & control; D013997:Time Factors
D008978:Mollusk Venoms; D000109:Acetylcholine