title: Piezo1 and Piezo2 are essential components of distinct mechanically activated cation channels.
abstract: Mechanical stimuli drive many physiological processes, including touch and pain sensation, hearing, and blood pressure regulation. Mechanically activated (MA) cation channel activities have been recorded in many cells, but the responsible molecules have not been identified. We characterized a rapidly adapting MA current in a mouse neuroblastoma cell line. Expression profiling and RNA interference knockdown of candidate genes identified Piezo1 (Fam38A) to be required for MA currents in these cells. Piezo1 and related Piezo2 (Fam38B) are vertebrate multipass transmembrane proteins with homologs in invertebrates, plants, and protozoa. Overexpression of mouse Piezo1 or Piezo2 induced two kinetically distinct MA currents. Piezos are expressed in several tissues, and knockdown of Piezo2 in dorsal root ganglia neurons specifically reduced rapidly adapting MA currents. We propose that Piezos are components of MA cation channels.
authors.affiliation: Department of Cell Biology, The Scripps Research Institute (TSRI), La Jolla, CA 92037, USA.
authors.forename: Bertrand
authors.initials: B
authors.lastname: Coste
authors.forename: Jayanti
authors.initials: J
authors.lastname: Mathur
authors.forename: Manuela
authors.initials: M
authors.lastname: Schmidt
authors.forename: Taryn J
authors.initials: TJ
authors.lastname: Earley
authors.forename: Sanjeev
authors.initials: S
authors.lastname: Ranade
authors.forename: Matt J
authors.initials: MJ
authors.lastname: Petrus
authors.forename: Adrienne E
authors.initials: AE
authors.lastname: Dubin
authors.forename: Ardem
authors.initials: A
authors.lastname: Patapoutian
mesh_terms: D000818:Animals; D002412:Cations / Q000378:metabolism*; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000737:chemistry; D003001:Cloning, Molecular; D005727:Ganglia, Spinal / Q000166:cytology; D007473:Ion Channels / Q000032:analysis / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D040542:Mechanotransduction, Cellular; D008564:Membrane Potentials; D051379:Mice; D008969:Molecular Sequence Data; D009474:Neurons / Q000378:metabolism*; D018408:Patch-Clamp Techniques; D011312:Pressure; D017434:Protein Structure, Tertiary; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D014162:Transfection
chemical_list: D002412:Cations; D007473:Ion Channels; C552920:Piezo1 protein, mouse; C552921:Piezo2 protein, mouse; D034741:RNA, Small Interfering
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