Fluorinated diaminocyclopentanes as chiral sensitive NMR probes of RNA structure.
The supramolecular chiral recognition between rac-2a and several structured RNA leads to a distinct (19)F NMR signal splitting. The (19)F NMR analysis of the diastereomeric pairs formed upon binding of this racemic probe delivers a topological footprint of the RNA. This phenomenon can be exploited to investigate dynamic events involving structural equilibria, as demonstrated in a melting experiment. This work provides a proof of concept that small fluorinated moderate binders can act as external probes of RNA structures.
Moumné
Roba
R
UMR8638 and UMR8015, CNRS-Paris Descartes University, Faculté de Pharmacie, 4 av. de l'Observatoire, 75006 Paris, France.
Pasco
Morgane
M
Prost
Elise
E
Lecourt
Thomas
T
Micouin
Laurent
L
Tisné
Carine
C
D001483:Base Sequence; D003517:Cyclopentanes / Q000737:chemistry*; D005461:Fluorine / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D015335:Molecular Probes; D009690:Nucleic Acid Conformation; D012313:RNA / Q000737:chemistry*
D003517:Cyclopentanes; D015335:Molecular Probes; D005461:Fluorine; D012313:RNA