title: Studies on MAP Kinase signaling in the immune system.
abstract: The primary function of the immune system is to protect the organism from invading pathogens. In response to pathogen invasion, multiple signaling pathways are activated in immune cells, leading to diverse immune defense mechanisms. Chief among these pathways is the activation of MAPKs, which are crucial for transcriptional and nontranscriptional responses of the immune system. Here we describe protocols to study the roles of MAPKs in T lymphocytes, a cell type central for immune regulation. Specifically, we describe flow cytometry-based assays to analyze the roles of MAPKs in the development, homeostasis, proliferation, and apoptosis of murine T cells. We also describe methods to examine the activation of MAPKs in T cells.
authors.affiliation: Department of Immunology, St Jude Children's Research Hospital, Memphis, TN, USA. hongbo.chi@stjude.org
authors.forename: Hongbo
authors.initials: H
authors.lastname: Chi
authors.forename: Richard A
authors.initials: RA
authors.lastname: Flavell
mesh_terms: D000818:Animals; D017304:Annexin A5 / Q000378:metabolism; D017209:Apoptosis; D015704:CD4 Antigens / Q000378:metabolism; D049109:Cell Proliferation; D002469:Cell Separation; D005434:Flow Cytometry; D005452:Fluoresceins / Q000378:metabolism; D006706:Homeostasis; D007107:Immune System / Q000166:cytology* / Q000201:enzymology; D020935:MAP Kinase Signaling System; D051379:Mice; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D010750:Phosphoproteins / Q000378:metabolism; D013194:Staining and Labeling; D013388:Succinimides / Q000378:metabolism; D013601:T-Lymphocytes / Q000166:cytology / Q000378:metabolism
chemical_list: C087165:5-(6)-carboxyfluorescein diacetate succinimidyl ester; D017304:Annexin A5; D015704:CD4 Antigens; D005452:Fluoresceins; D010750:Phosphoproteins; D013388:Succinimides; D020928:Mitogen-Activated Protein Kinases
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