title: [Cloning of keratinocyte growth factor 2 gene (KGF2) and its transformation to Brassica napus L].
abstract: Recently, more research about the plant bioreactor expressing genes encoding human proteins was reported. In the present study, the cDNA of the human gene keratinocyte growth factor 2 (KGF2) was replaced with plant preferred codons by PCR, and the modified full-length cDNA was cloned into the plant expression vector pCAMBIA-YO containing the oil-body promoter. The fusion construct pCAMBIA-YO-KGF2 was transformed into Brassica napus by Agrobacterium tumefacien-mediated cotyledon transformation method. The transgenic seedlings were identified by PCR, Southern and western blot analysis all showed that KGF2 gene was successfully expressed in in transgenic Brassica napus.
authors.affiliation: Engineering Research Center of Bioreactor and Pharmaceutical Development, Ministry of Education, Jilin Agricultural University, Changchun 130118, China.
authors.forename: Guoqing
authors.initials: G
authors.lastname: Pan
authors.forename: Shuang
authors.initials: S
authors.lastname: Zhang
authors.forename: Xiuming
authors.initials: X
authors.lastname: Liu
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.forename: Yaofang
authors.initials: Y
authors.lastname: Zhang
authors.forename: Hongzhi
authors.initials: H
authors.lastname: Li
authors.forename: Haiyan
authors.initials: H
authors.lastname: Li
authors.forename: Xiaokun
authors.initials: X
authors.lastname: Li
mesh_terms: D029688:Brassica napus / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D051523:Fibroblast Growth Factor 7 / Q000096:biosynthesis / Q000235:genetics*; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D030821:Plants, Genetically Modified / Q000235:genetics*; D012231:Rhizobium / Q000235:genetics; D014170:Transformation, Genetic
chemical_list: D018076:DNA, Complementary; C496453:FGF7 protein, human; D051523:Fibroblast Growth Factor 7