[GABAergic neurons in the central nucleus of amygdala modulate sodium appetite in rats].
OBJECTIVE
To determine whether the GABA-containing neurons in rat central nucleus of amygdala (CeA) can be activated by acute sodium deprivation.


METHODS
Acute sodium depletion was induced by subcutaneous injection of furosemide in rats followed by 24 h of dietary sodium deprivation. The rats underwent 0.3 mol/L NaCl/distilled water two bottle choice test, and the activated neurons were labeled and identified with GABA/Fos-double labeling immunohistochemistry.


RESULTS
The rats with acute sodium depletion exhibited significantly more numerous c-fos-positive neurons and GABA/Fos double-labeled neurons in the CeA than the control group (P<0.01, P<0.05). Consumption of 0.3 mol/L NaCl significantly increased the number of c-fos and GABA/Fos double labeled neurons compared to the distilled water group (P<0.001, P<0.01).


CONCLUSIONS
GABAergic neurons in the CeA may play an inhibitory role in the regulation of sodium intake in rats with acute sodium depletion.
Wang
Qian
Q
Department of Physiology and Pathophysiology, Xi'an Jiaotong University College of Medicine, Xi'an 710061, China.
Yan
Jian-qun
JQ
Li
Jin-rong
JR
Chen
Ke
K
Sun
Bo
B
Zhao
Shi-ru
SR
D000679:Amygdala / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D059330:GABAergic Neurons / Q000378:metabolism*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D017673:Sodium Chloride, Dietary / Q000378:metabolism*; D012982:Sodium, Dietary
D017673:Sodium Chloride, Dietary; D012982:Sodium, Dietary