Proteomic analysis of germinating urediniospores of Phakopsora pachyrhizi, causal agent of Asian soybean rust.
Phakopsora pachyrhizi is an obligate pathogen that causes Asian soybean rust. Asian soybean rust has an unusually broad host range and infects by direct penetration through the leaf cuticle. In order to understand the early events in the infection process, it is important to identify and characterize proteins in P. pachyrhizi. Germination of the urediniospore is the first stage in the infection process and represents a critical life stage applicable to studies with this obligate pathogen. We have applied a 2-DE and MS approach to identify 117 proteins from the National Center of Biotechnology Information nonredundant protein database and a custom database of Basidiomycota EST sequences. Proteins with roles in primary metabolism, energy transduction, stress, cellular regulation and signaling were identified in this study. This data set is accessible at http://world-2dpage.expasy.org/repository/database=0018.
Luster
Douglas G
DG
Foreign Disease-Weed Science Research Unit, US Department of Agriculture, Agricultural Research Service, MD 21702, USA. Doug.Luster@ars.usda.gov
McMahon
Michael B
MB
Carter
Melissa L
ML
Fortis
Laurie L
LL
Nuñez
Alberto
A
D000595:Amino Acid Sequence; D001487:Basidiomycota / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D005656:Fungal Proteins / Q000378:metabolism*; D018525:Germination; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000382:microbiology*; D020543:Proteome / Q000378:metabolism*; D013025:Glycine max / Q000382:microbiology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
D005656:Fungal Proteins; D020543:Proteome