title: Fibronectin and integrin alpha 5 play essential roles in the development of the cardiac neural crest.
abstract: Cardiac neural crest (CNC) plays a requisite role during cardiovascular development and defects in the formation of CNC-derived structures underlie several common forms of human congenital birth defects. Migration of the CNC cells to their destinations as well as expansion and maintenance of these cells are important for the normal development of the cardiac outflow tract and aortic arch arteries; however, molecular mechanisms regulating these processes are not well-understood. Fibronectin (FN) protein is present along neural crest migration paths and neural crest cells migrate when plated on FN in vitro; therefore, we tested the role of FN during the development of the CNC in vivo. Our analysis of the fate of the neural crest shows that CNC cells reach their destinations in the branchial arches and the cardiac outflow tract in the absence of FN or its cellular receptor integrin α5β1. However, we found that FN and integrin α5 modulate CNC proliferation and survival, and are required for the presence of normal numbers of CNC cells at their destinations.
authors.affiliation: Weill Cornell Medical College, Department of Medicine, Division of Cardiology, New York, NY, USA.
authors.forename: Ashok
authors.initials: A
authors.lastname: Mittal
authors.forename: Maria
authors.initials: M
authors.lastname: Pulina
authors.forename: Shuan-Yu
authors.initials: SY
authors.lastname: Hou
authors.forename: Sophie
authors.initials: S
authors.lastname: Astrof
mesh_terms: D000818:Animals; D017209:Apoptosis; D015415:Biomarkers / Q000378:metabolism; D019521:Body Patterning / Q000235:genetics; D001934:Branchial Region / Q000166:cytology; D002452:Cell Count; D019070:Cell Lineage / Q000235:genetics; D002465:Cell Movement; D049109:Cell Proliferation; D002470:Cell Survival; D004622:Embryo, Mammalian / Q000166:cytology / Q000378:metabolism; D005353:Fibronectins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D006321:Heart / Q000196:embryology*; D039482:Integrin alpha5 / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D009206:Myocardium / Q000166:cytology; D009432:Neural Crest / Q000166:cytology / Q000196:embryology* / Q000378:metabolism*; D054259:Neural Tube / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism; D013997:Time Factors; D015854:Up-Regulation / Q000235:genetics
chemical_list: D015415:Biomarkers; D005353:Fibronectins; D039482:Integrin alpha5; D012333:RNA, Messenger
references.citation: Dev Biol. 2006 Jan 1;289(1):218-28
references.pmid: 16316641
references.citation: J Cell Biol. 2000 Jul 24;150(2):F89-96
references.pmid: 10908592
references.citation: Dev Biol. 1982 Oct;93(2):308-23
references.pmid: 7141100
references.citation: Development. 1992 Oct;116(2):297-307
references.pmid: 1283734
references.citation: Dev Biol. 2003 Aug 15;260(2):449-64
references.pmid: 12921745
references.citation: Development. 2006 Jun;133(12):2419-33
references.pmid: 16720879
references.citation: Blood. 1997 Oct 15;90(8):3073-81
references.pmid: 9376588
references.citation: Dev Biol. 2003 Jul 15;259(2):288-302
references.pmid: 12871702
references.citation: Development. 1997 Nov;124(21):4309-19
references.pmid: 9334279
references.citation: Proc Natl Acad Sci U S A. 1995 Jun 20;92(13):6161-5
references.pmid: 7541142
references.citation: Proc Natl Acad Sci U S A. 1999 Mar 16;96(6):2805-10
references.pmid: 10077592
references.citation: Development. 1993 Dec;119(4):1079-91
references.pmid: 8306876
references.citation: Cardiovasc Res. 2000 Aug;47(2):314-28
references.pmid: 10946068
references.citation: J Biol Chem. 2001 Jul 27;276(30):27757-63
references.pmid: 11333270
references.citation: Development. 1987 Nov;101(3):461-78
references.pmid: 3332259
references.citation: Dev Biol. 2008 Jul 15;319(2):336-45
references.pmid: 18538758
references.citation: Development. 1994 Sep;120(9):2687-702
references.pmid: 7525179
references.citation: Dev Biol. 1981 Mar;82(2):267-86
references.pmid: 7014292
references.citation: Cell. 1998 Nov 13;95(4):507-19
references.pmid: 9827803
references.citation: Curr Opin Genet Dev. 2001 Feb;11(1):48-53
references.pmid: 11163150
references.citation: Development. 1993 Aug;118(4):1255-66
references.pmid: 8269852
references.citation: Dev Biol. 2001 May 1;233(1):38-55
references.pmid: 11319856
references.citation: Development. 2005 Dec;132(23):5307-15
references.pmid: 16284121
references.citation: Mol Biol Cell. 1999 Oct;10(10):3197-204
references.pmid: 10512860
references.citation: Annu Rev Cell Dev Biol. 2006;22:267-86
references.pmid: 16803431
references.citation: EMBO J. 1991 May;10(5):1135-47
references.pmid: 2022185
references.citation: Development. 2009 Sep;136(18):3173-83
references.pmid: 19700621
references.citation: Dev Biol. 2006 Dec 1;300(1):252-66
references.pmid: 16950242
references.citation: Dev Biol. 1999 Apr 15;208(2):307-23
references.pmid: 10191047
references.citation: Dev Dyn. 1996 Oct;207(2):145-56
references.pmid: 8906418
references.citation: J Cell Biol. 1983 Feb;96(2):462-73
references.pmid: 6833366
references.citation: Development. 1993 Dec;119(4):1093-105
references.pmid: 7508365
references.citation: Development. 2004 Jul;131(14):3367-79
references.pmid: 15226254
references.citation: J Cell Sci. 2006 Oct 1;119(Pt 19):3901-3
references.pmid: 16988024
references.citation: Cell. 1997 Mar 7;88(5):603-13
references.pmid: 9054500
references.citation: Dev Biol. 2005 Apr 15;280(2):396-406
references.pmid: 15882581
references.citation: Semin Cell Dev Biol. 2007 Feb;18(1):101-10
references.pmid: 17224285
references.citation: J Cell Sci. 1999 Dec;112 ( Pt 24):4715-28
references.pmid: 10574719
references.citation: Development. 2003 Dec;130(25):6361-74
references.pmid: 14623825
references.citation: Nature. 1995 Jun 29;375(6534):787-90
references.pmid: 7596411
references.citation: Nat Genet. 1999 Jan;21(1):70-1
references.pmid: 9916792
references.citation: J Clin Invest. 2009 Jul;119(7):1986-96
references.pmid: 19509466
references.citation: Development. 2004 May;131(9):2205-18
references.pmid: 15073157
references.citation: Nature. 2005 Feb 24;433(7028):884-7
references.pmid: 15729346
references.citation: Development. 2000 Apr;127(8):1607-16
references.pmid: 10725237
references.citation: Development. 2008 Nov;135(21):3599-610
references.pmid: 18832392
references.citation: J Cell Sci. 2001 Jul;114(Pt 14):2553-60
references.pmid: 11683383
references.citation: Development. 2007 Apr;134(8):1593-604
references.pmid: 17344228
references.citation: Cell Adhes Commun. 1996 Aug;4(2):103-25
references.pmid: 8937746
references.citation: Development. 2006 Sep;133(18):3629-39
references.pmid: 16914489
references.citation: Dev Biol. 1999 Nov 15;215(2):264-77
references.pmid: 10545236