title: Effect of pancreatic tissue sampling on serum pancreatic enzyme levels in clinically healthy dogs.
abstract: Little is known about the potential consequences of pancreatic tissue sampling in dogs. The goal of the present study was to evaluate changes in serum trypsin-like immunoreactivity and canine-specific pancreatic lipase after pancreatic fine-needle aspiration and surgical biopsy in 27 clinically healthy dogs. Presurgical, ultrasound-guided aspiration of the pancreas was performed with the dogs under sedation. Subsequently, all the dogs underwent intraoperative pancreatic fine-needle aspiration and clamshell biopsy. After euthanasia, pancreata were sectioned for histopathologic evaluation. Serum pancreatic enzyme levels were measured at 3 time points: baseline, after ultrasound-guided aspiration, and after intraoperative aspiration and biopsy. No significant differences were detected among mean serum pancreatic lipase values at any point (P > 0.05). Serum trypsin-like immunoreactivity did not change from baseline (18.2 +/- 2.1 microg/dl; mean +/- standard error) after ultrasound-guided aspiration (13.6 +/- 2.2 microg/dl) but increased significantly after intraoperative sampling (44.8 +/- 1.9 microg/dl; P < 0.0005). After surgical biopsy, the 20 dogs that had both ultrasound-guided and intraoperative sampling had a higher mean (SE) serum trypsin-like immunoreactivity (44.8 +/- 1.9 microg/dl) than the 7 dogs that had only intraoperative samples taken (36.4 +/- 4.1 microg/dl; P < 0.05). All 27 pancreata were grossly normal before intraoperative sampling. Pancreatic sampling was associated with increased serum trypsin-like immunoreactivity and mild, peracute necrosis, inflammation, hemorrhage, and fibrin deposition. Tissue damage from sampling was not sufficient to cause an elevation in canine-specific pancreatic lipase in the time frame evaluated. Further studies are needed to determine longer-term effects of pancreatic sampling on enzyme levels and clinical outcome.
authors.affiliation: Veterinary Clinical Sciences, College of Veterinary Medicine, University of Minnesota, Saint Paul, MN 55108, USA.
authors.forename: Amy P
authors.initials: AP
authors.lastname: Cordner
authors.forename: P Jane
authors.initials: PJ
authors.lastname: Armstrong
authors.forename: Shelley J
authors.initials: SJ
authors.lastname: Newman
authors.forename: Roberto
authors.initials: R
authors.lastname: Novo
authors.forename: Leslie C
authors.initials: LC
authors.lastname: Sharkey
authors.forename: Carl
authors.initials: C
authors.lastname: Jessen Emeritus
mesh_terms: D000818:Animals; D001706:Biopsy / Q000662:veterinary; D044963:Biopsy, Fine-Needle; D001772:Blood Cell Count / Q000662:veterinary; D004283:Dog Diseases / Q000097:blood / Q000201:enzymology; D004285:Dogs / Q000097:blood*; D019160:Endosonography; D004798:Enzymes / Q000097:blood*; D008049:Lipase / Q000097:blood / Q000378:metabolism; D010179:Pancreas / Q000166:cytology / Q000201:enzymology* / Q000473:pathology; D010195:Pancreatitis / Q000201:enzymology / Q000662:veterinary; D012016:Reference Values; D014357:Trypsin / Q000097:blood / Q000378:metabolism
chemical_list: D004798:Enzymes; D008049:Lipase; D014357:Trypsin