Erythrocyte survival time in Greyhounds as assessed by use of in vivo biotinylation.
OBJECTIVE
To determine erythrocyte survival time in Greyhounds.


METHODS
6 Greyhounds used as blood donors and 3 privately owned non-Greyhound dogs.


METHODS
In vivo biotinylation of erythrocytes was performed by infusion of biotin-N-hydroxysuccinimide into each dog via a jugular vein catheter. Blood samples were collected 12 hours later and then at weekly intervals and were used to determine the percentage of biotin-labeled erythrocytes at each time point. Erythrocytes were washed, incubated with avidin-fluorescein isothiocyanate, and washed again before the percentage of biotinylated erythrocytes was measured by use of flow cytometry. Survival curves for the percentage of biotinylated erythrocytes were generated, and erythrocyte survival time was defined as the x-intercept of a least squares best-fit line for the linear portion of each curve.


RESULTS
The R2 for survival curves ranged from 0.93 to 0.99 during the first 10 weeks after infusion of erythrocytes. Erythrocyte survival time for the 3 non-Greyhound dogs was 94, 98, and 116 days, respectively, which was consistent with previously reported values. Erythrocyte survival time for the 6 Greyhounds ranged from 83 to 110 days (mean, 93 days; median, 88 days). As determined by use of in vivo biotinylation, erythrocyte survival times in Greyhounds were similar to those determined for non-Greyhound dogs and did not differ significantly from erythrocyte survival times reported previously for non-Greyhound dogs.


CONCLUSIONS
Erythrocyte survival time was similar in Greyhounds and non-Greyhound dogs. Greyhounds can be used as erythrocyte donors without concerns about inherently shorter erythrocyte survival time.
Garon
Catherine L
CL
Department of Veterinary Medicine and Surgery, College of Veterinary Medicine, University of Missouri, Columbia, MO 65211, USA.
Cohn
Leah A
LA
Scott
Michael A
MA
D000818:Animals; D001710:Biotin / Q000031:analogs & derivatives* / Q000097:blood / Q000494:pharmacology; D019921:Biotinylation / Q000662:veterinary*; D001782:Blood Donors; D001831:Body Temperature / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000166:cytology* / Q000187:drug effects; D005260:Female; D005434:Flow Cytometry; D007700:Kinetics; D008297:Male; D012016:Reference Values; D012881:Skin Temperature / Q000187:drug effects; D013388:Succinimides / Q000097:blood / Q000494:pharmacology*
D013388:Succinimides; C053827:biotinyl-N-hydroxysulfosuccinimide ester; D001710:Biotin