title: Assessment of contamination of the Svitava and Svratka rivers in the Czech Republic using selected biochemical markers.
abstract: The aim of the present study is to assess aquatic ecosystem contamination using selected biochemical markers: cytochrome P450, ethoxyresorufin-O-deethylase (EROD), glutathione S-transferase (GST), tripeptide glutathione, vitellogenin, and 11-ketotestosterone in chub (Leuciscus cephalus L.). Seven locations on the Svitava and Svratka rivers (in the Brno conurbation, Czech Republic) were assessed. The results were compared with the levels of the most important inductors of these biomarkers: organic pollutants hexachlorocyclohexane (HCH), hexachlorobenzene (HCB), DDT and its metabolites, polychlorinated biphenyls, and polycyclic aromatic hydrocarbons in bottom sediment, fish muscle, and semipermeable membrane devices (SPMDs) and metals in sediment. The highest levels of pollutants were observed at sites situated downstream from Brno, especially at Modrice and Rajhradice. Significant positive correlations (p < 0.05) were found between EROD activity and HCH concentration in SPMDs, and also between GST and EROD activity with HCB concentration in muscle, after adjusting for age.
authors.affiliation: Department of Veterinary Public Health and Toxicology, University of Veterinary and Pharmaceutical Sciences Brno, Palackého 1-3, Brno, Czech Republic. blahovaj@vfu.cz
authors.forename: Jana
authors.initials: J
authors.lastname: Blahová
authors.forename: Marcela
authors.initials: M
authors.lastname: Havelková
authors.forename: Kamila
authors.initials: K
authors.lastname: Kruzíková
authors.forename: Klára
authors.initials: K
authors.lastname: Hilscherová
authors.forename: Roman
authors.initials: R
authors.lastname: Halouzka
authors.forename: Helena
authors.initials: H
authors.lastname: Modrá
authors.forename: Roman
authors.initials: R
authors.lastname: Grabic
authors.forename: Jarmila
authors.initials: J
authors.lastname: Halírová
authors.forename: Jana
authors.initials: J
authors.lastname: Jurcíková
authors.forename: Tomás
authors.initials: T
authors.lastname: Ocelka
authors.forename: Danka
authors.initials: D
authors.lastname: Harustiaková
authors.forename: Zdenka
authors.initials: Z
authors.lastname: Svobodová
mesh_terms: D000818:Animals; D015415:Biomarkers; D003530:Cyprinidae / Q000378:metabolism*; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism; D018153:Czech Republic; D052244:Endocrine Disruptors / Q000032:analysis; D004784:Environmental Monitoring; D005982:Glutathione Transferase / Q000378:metabolism; D008297:Male; D009930:Organic Chemicals / Q000032:analysis; D045483:Rivers / Q000737:chemistry*; D013739:Testosterone / Q000031:analogs & derivatives / Q000097:blood; D014819:Vitellogenins / Q000097:blood; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D015415:Biomarkers; D052244:Endocrine Disruptors; D009930:Organic Chemicals; D014819:Vitellogenins; D014874:Water Pollutants, Chemical; D013739:Testosterone; D019363:Cytochrome P-450 CYP1A1; D005982:Glutathione Transferase; C003600:11-ketotestosterone