title: Simian immunodeficiency virus-specific CD4+ T cells from successful vaccinees target the SIV Gag capsid.
abstract: We recently demonstrated that vaccinated rhesus macaques controlled viral replication of a heterologous SIV challenge. Here, we analyzed anamnestic SIV-specific CD4+ T-cell responses expanding immediately after challenge and show that successful vaccinees consistently targeted a short region of the Gag-p27 Capsid (amino acids 249-291). We have also defined the major histocompatibility complex class II (MHC-II) restricting alleles for several of these responses and show that DQ-restricted CD4+ T-cells depend on unique combinations of both the DQA and DQB alleles. Analysis of SIV-specific CD4+ T-cell responses elicited by a successful vaccine may have important implications in the understanding of vaccine design.
authors.affiliation: Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, WI 53711, USA.
authors.forename: Juan P
authors.initials: JP
authors.lastname: Giraldo-Vela
authors.forename: Alex T
authors.initials: AT
authors.lastname: Bean
authors.forename: Richard
authors.initials: R
authors.lastname: Rudersdorf
authors.forename: Lyle T
authors.initials: LT
authors.lastname: Wallace
authors.forename: John T
authors.initials: JT
authors.lastname: Loffredo
authors.forename: Priscilla
authors.initials: P
authors.lastname: Erickson
authors.forename: Nancy A
authors.initials: NA
authors.lastname: Wilson
authors.forename: David I
authors.initials: DI
authors.lastname: Watkins
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D000939:Epitopes / Q000276:immunology; D015683:Gene Products, gag / Q000276:immunology*; D005802:Genes, MHC Class II; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D007156:Immunologic Memory; D008253:Macaca mulatta / Q000235:genetics / Q000276:immunology*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000276:immunology; D012191:Retroviridae Proteins / Q000276:immunology; D018449:SAIDS Vaccines / Q000276:immunology*; D016097:Simian Acquired Immunodeficiency Syndrome / Q000276:immunology / Q000517:prevention & control; D015302:Simian Immunodeficiency Virus / Q000276:immunology* / Q000302:isolation & purification / Q000502:physiology; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D014611:Vaccination; D019562:Viral Load; D014766:Viremia / Q000276:immunology / Q000517:prevention & control; D014779:Virus Replication
chemical_list: D000939:Epitopes; C066594:Gag protein p27, Simian immunodeficiency virus; D015683:Gene Products, gag; D000949:Histocompatibility Antigens Class II; D010446:Peptide Fragments; D012191:Retroviridae Proteins; D018449:SAIDS Vaccines
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