title: [Preparation and verification of antibodies for five rice receptor-like kinases].
abstract: Receptor-like kinase involves self-incompatibility, male sterility, stress responses, and disease resistance. To better understand the physiological function and biological characteristics of rice receptor-like kinase, we cloned five predicted epitope fragments of rice receptor-like kinase. The purified fusion protein was used as antigen to immunize rabbit to get specific polyclonal antibodies. Western blotting analysis shows that the five receptor-like kinases were expressed in rice leaves.
authors.affiliation: College of Life Sciences, Agricultural University of Hebei, Baoding 071001, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Wang
authors.forename: Liyun
authors.initials: L
authors.lastname: Li
authors.forename: Yinghao
authors.initials: Y
authors.lastname: Cao
authors.forename: Ziguang
authors.initials: Z
authors.lastname: Liu
authors.forename: Guozhen
authors.initials: G
authors.lastname: Liu
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology; D003001:Cloning, Molecular; D000939:Epitopes / Q000276:immunology; D007114:Immunization; D012275:Oryza / Q000201:enzymology* / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics / Q000276:immunology*; D046549:Plantibodies / Q000276:immunology* / Q000378:metabolism; D011494:Protein Kinases / Q000235:genetics / Q000276:immunology*; D011817:Rabbits
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D010940:Plant Proteins; D046549:Plantibodies; D011494:Protein Kinases