Long-term human pluripotent stem cell self-renewal on synthetic polymer surfaces.
Realization of the full potential of human pluripotent stem cells (hPSCs) in regenerative medicine requires the development of well-defined culture conditions for their long-term growth and directed differentiation. Current practices for maintaining hPSCs generally utilize empirically determined combinations of feeder cells and other animal-based products, which are expensive, difficult to isolate, subject to batch-to-batch variations, and unsuitable for cell-based therapies. Using a high-throughput screening approach, we identified several polymers that can support self-renewal of hPSCs. While most of these polymers provide support for only a short period of time, we identified a synthetic polymer poly(methyl vinyl ether-alt-maleic anhydride) (PMVE-alt-MA) that supported the long-term attachment, proliferation and self-renewal of HUES1, HUES9, and iPSCs. The hPSCs cultured on PMVE-alt-MA maintained their characteristic morphology, expressed high levels of markers of pluripotency, and retained a normal karyotype. Such cost-effective, polymer-based matrices that support long-term self-renewal and proliferation of hPSCs will not only help to accelerate the translational perspectives of hPSCs, but also provide a platform to elucidate the underlying molecular mechanisms that regulate stem cell proliferation and differentiation.
Brafman
David A
DA
Department of Bioengineering, University of California-San Diego, 9500 Gilman Dr, La Jolla, CA 92093-0695, United States.
Chang
Chien W
CW
Fernandez
Antonio
A
Willert
Karl
K
Varghese
Shyni
S
Chien
Shu
S
D019070:Cell Lineage / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D053595:Embryonic Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D016023:Integrins / Q000378:metabolism; D008299:Maleic Anhydrides / Q000494:pharmacology; D008738:Methyl Ethers / Q000494:pharmacology; D046228:Microarray Analysis; D008970:Molecular Weight; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D011108:Polymers / Q000737:chemistry / Q000494:pharmacology*; D011145:Polyvinyls / Q000494:pharmacology; D013499:Surface Properties / Q000187:drug effects; D013997:Time Factors
D016326:Extracellular Matrix Proteins; D016023:Integrins; D008299:Maleic Anhydrides; D008738:Methyl Ethers; D011108:Polymers; D011145:Polyvinyls; C510739:poly(vinyl methyl ether)
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