title: Dicer is a key player in oocyte maturation.
abstract: OBJECTIVE
  Apply Dicer siRNA to study functions of Dicer and miRNA during oogenesis.
  
  
  METHODS
  Mouse oocytes were injected with Dicer siRNA and negative control siRNA and then matured in vitro. After IVM, oocytes were examined for maturation rates, spindle and chromosomal organization, and various gene expressions.
  
  
  RESULTS
  Dicer siRNA significantly reduced maturation rates, increased abnormal spindle and chromosomal organization, and reduced the transcripts of Dicer miRNAs, spindle formation proteins (plk1 and AURKA) and spindle check points (Bub1, Bublb). Depletion of bulb16 markedly prohibited the first polar body extrusion and increased the incidence of misaligned chromosomes and abnormal meiotic spindle assembly.
  
  
  CONCLUSIONS
  Dicer siRNA triggered a cascade reduction for gene expressions starting from Dicer to miRNAs than to spindle assembly proteins and checkpoints which led to abnormal spindle and chromosomal organization. Thus, Dicer and miRNA appeared to play an important role during oogenesis and were essential for meiotic completion.
authors.affiliation: Department of Reproductive Medicine, Weill Medical College of Cornell University, New York, NY 10021, USA. hcliu@med.cornell.edu
authors.forename: Hung-Ching
authors.initials: HC
authors.lastname: Liu
authors.forename: YaXu
authors.initials: Y
authors.lastname: Tang
authors.forename: Zhiying
authors.initials: Z
authors.lastname: He
authors.forename: Zev
authors.initials: Z
authors.lastname: Rosenwaks
mesh_terms: D000818:Animals; D016009:Chi-Square Distribution; D053487:DEAD-box RNA Helicases / Q000378:metabolism*; D004722:Endoribonucleases / Q000378:metabolism*; D005260:Female; D005455:Fluorescent Antibody Technique; D008540:Meiosis / Q000502:physiology*; D051379:Mice; D008845:Microinjections; D018613:Microscopy, Confocal; D009866:Oogenesis / Q000502:physiology*; D034741:RNA, Small Interfering; D020133:Reverse Transcriptase Polymerase Chain Reaction; D043244:Ribonuclease III
chemical_list: D034741:RNA, Small Interfering; D004722:Endoribonucleases; C509781:Dicer1 protein, mouse; D043244:Ribonuclease III; D053487:DEAD-box RNA Helicases
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