title: Mineralization of isoproturon, mecoprop and acetochlor in a deep unsaturated limestone and sandy aquifer.
abstract: Isoproturon (N,N-dimethyl-N'-[4-(1-methylethyl)phenyl]urea), mecoprop (MCPP) (2-(4-chloro-2-methylphenoxy)propanoic acid) and acetochlor (2-chloro-N-(2-ethoxymethyl)-N-(2-ethyl-6-methylphenyl)acetamide) are agricultural pesticides that may leach through the vadose zone down to groundwater. Sediment samples were collected from intact sediment cores from 0 to 59 m below surface, including soil, unsaturated limestone and aquifer sand. In the unsaturated limestone, the initial pesticide concentrations (0.5-100 μg kg(-1)) did not systematically affect the proportion of mineralized pesticides or the kinetics. However, in the aquifer, mecoprop and to some degree isoproturon mineralization was found to increase with increasing initial concentration (0.5-100 μg L(-1) equivalent to 1-220 μg kg(-1)) demonstrating the importance of using environmentally relevant concentrations when predicting pesticide fate. The mineralization of isoproturon, mecoprop and acetochlor was studied in 40 samples at low concentrations (1-3 μg L(-1)) and specific pesticide-mineralizing bacteria were enumerated using 14C-MPN. Presence of the mineralizers documented a degradation potential of the pesticides within the catchment. The number of mineralizers varied from <0.18 to >16000 g(-1) and was not found to correlate with depth. Mecoprop, isoproturon and acetochlor were substantially mineralized in the soils (19-44% after 8months incubation at 1 μg kg(-1)), in sub-surface unsaturated limestone samples (≤2% for acetochlor, ≤21% for isoproturon and ≤31% for mecoprop) and in aquifer samples (4-28% for mecoprop, ≤4.7% for isoproturon and ≤5.6% for acetochlor). The finding of isoproturon and acetochlor mineralization in deep aquifers is novel and important for the evaluation of the fate of these pesticides, as even low mineralization rates can be important in aquifers exhibiting long residence times.
authors.affiliation: DTU Environment, Department of Environmental Engineering, Technical University of Denmark, Miljoevej, Building 113, DK-2800 Kgs. Lyngby, Denmark. gsja@env.dtu.dk
authors.forename: G S
authors.initials: GS
authors.lastname: Janniche
authors.forename: E
authors.initials: E
authors.lastname: Lindberg
authors.forename: C
authors.initials: C
authors.lastname: Mouvet
authors.forename: H-J
authors.initials: HJ
authors.lastname: Albrechtsen
mesh_terms: D008456:2-Methyl-4-chlorophenoxyacetic Acid / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry; D002119:Calcium Carbonate / Q000737:chemistry; D004784:Environmental Monitoring; D005618:Fresh Water / Q000737:chemistry; D019015:Geologic Sediments / Q000737:chemistry; D006540:Herbicides / Q000032:analysis / Q000737:chemistry*; D007700:Kinetics; D008903:Minerals / Q000737:chemistry; D010671:Phenylurea Compounds / Q000032:analysis / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry; D014052:Toluidines / Q000032:analysis / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000737:chemistry*
chemical_list: D006540:Herbicides; D008903:Minerals; D010671:Phenylurea Compounds; D014052:Toluidines; D014874:Water Pollutants, Chemical; C028904:isoproturon; C008340:mecoprop; D012822:Silicon Dioxide; C043377:acetochlor; D008456:2-Methyl-4-chlorophenoxyacetic Acid; D002119:Calcium Carbonate