Interaction of n-octyl β,D-glucopyranoside with giant magnetic-fluid-loaded phosphatidylcholine vesicles: direct visualization of membrane curvature fluctuations as a function of surfactant partitioning between water and lipid bilayer.
The present study deals with the morphological modifications of giant dioleoyl phosphatidylcholine vesicles (DOPC GUVs) induced by the nonionic surfactant n-octyl β,D-glucopyranoside at sublytic levels, i.e., in the first steps of the vesicle-to-micelle transition process, when surfactant inserts into the vesicle bilayer without disruption. Experimental conditions were perfected to exactly control the surfactant bilayer composition of the vesicles, in line with former work focused on the mechanical properties of the membrane of magnetic-fluid-loaded DOPC GUVs submitted to a magnetic field. The purpose here was to systematically examine, in the absence of any external mechanical constraint, the dynamics of giant vesicle shape and membrane deformations as a function of surfactant partitioning between the aqueous phase and the lipid membrane, beforehand established by turbidity measurements from small unilamellar vesicles.
Ménager
Christine
C
UPMC Univ Paris 06, UMR 7195 PECSA, Physicochimie des Electrolytes, Colloïdes, Sciences Analytiques, F-75005 Paris France.
Guemghar
Dihya
D
Cabuil
Valérie
V
Lesieur
Sylviane
S
D005960:Glucosides / Q000737:chemistry*; D008051:Lipid Bilayers; D008280:Magnetics; D046529:Microscopy, Electron, Transmission; D010713:Phosphatidylcholines / Q000737:chemistry*; D012995:Solubility; D013501:Surface-Active Agents / Q000737:chemistry*
D005960:Glucosides; D008051:Lipid Bilayers; D010713:Phosphatidylcholines; D013501:Surface-Active Agents; C018619:octyl-beta-D-glucoside