title: Multiple induced mutagenesis for improvement of ethanol production by Kluyveromyces marxianus.
abstract: Kluyveromyces marxianus GX-15 was mutated multiple times by alternately treatment with UV irradiation and NTG for two cycles. Four mutant strains with improved ethanol yield were obtained. The maximum ethanol concentration, ethanol yield coefficient and theoretical ethanol yield of the best mutant strain, GX-UN120, was 69 g/l, 0.46 g/g and 91%, respectively, when fermenting 150 g glucose/l at 40°C. The corresponding values for GX-15 were 58 g/l, 0.39 g/g and 76%, respectively. GX-UN120 grew well in 11% (v/v) of ethanol, while GX-15 could not grow when ethanol was greater than 8% (v/v).
authors.affiliation: College of Life Science and Technology, Guangxi University, Nanning, Guangxi, 530005, China.
authors.forename: Zong-wen
authors.initials: ZW
authors.lastname: Pang
authors.forename: Jing-juan
authors.initials: JJ
authors.lastname: Liang
authors.forename: Xiao-juan
authors.initials: XJ
authors.lastname: Qin
authors.forename: Ji-rui
authors.initials: JR
authors.lastname: Wang
authors.forename: Jia-xun
authors.initials: JX
authors.lastname: Feng
authors.forename: Ri-bo
authors.initials: RB
authors.lastname: Huang
mesh_terms: D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D007716:Kluyveromyces / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism* / Q000528:radiation effects; D053858:Metabolic Networks and Pathways / Q000235:genetics*; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009153:Mutagens / Q000378:metabolism; D009604:Nitrosoguanidines / Q000378:metabolism; D017422:Sequence Analysis, DNA; D014466:Ultraviolet Rays
chemical_list: D009153:Mutagens; D009604:Nitrosoguanidines; D000431:Ethanol; D005947:Glucose