Investigation of riboflavin sensitized degradation of purine and pyrimidine derivatives of DNA and RNA under UVA and UVB.
DNA and RNA undergo photodegradation in UVC (200-290nm) due to direct absorption by the purine and pyrimidine bases. Limited effects are observed under UVB (290-320nm) or UVA (320-400nm). We have observed that an endogenous photosensitizer, riboflavin (RF), upon exposure to UVB or UVA can extensively damage the DNA and RNA bases. Guanine, uracil, thymine, adenine and cytosine were degraded by 100%, 82%, 60.4%, 46.3% and 10.3% under UVA (12J) and by 100%, 54.1%, 38.9%, 42.2% and <1.0% under UVB (6J), respectively. Guanosine and deoxyguanosine were degraded by 98±1.0% and 80±1.0% under UVA (4J) and UVB (12J), respectively. With an exception of GMP (53-82%), dGMP (51-88%) and to some extent TMP (3-4%) the remaining nucleosides and nucleotides were resistant to RF-induced photodecomposition. The photodegradation of G derivatives by RF was 2-fold higher than a well known photodynamic agent rose bengal. A comparison of the intensities of UVA and UVB sources used in this study with natural sunlight suggests that exposure with the latter along with an endogenous photosensitizer can have similar effects on DNA and RNA depending upon the duration of exposure.
Joshi
Prakash C
PC
Department of Chemistry and Biochemistry, Russell Sage College, Troy, NY 12180, USA.
Keane
Thomas C
TC
D004247:DNA / Q000378:metabolism / Q000494:pharmacology / Q000528:radiation effects*; D004249:DNA Damage; D003849:Deoxyguanosine / Q000378:metabolism; D006151:Guanosine / Q000378:metabolism; D006801:Humans; D010782:Photolysis; D017319:Photosensitizing Agents / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D011687:Purines / Q000378:metabolism*; D011743:Pyrimidines / Q000378:metabolism*; D012313:RNA / Q000378:metabolism / Q000494:pharmacology / Q000528:radiation effects*; D017382:Reactive Oxygen Species / Q000378:metabolism; D012256:Riboflavin / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D012395:Rose Bengal / Q000494:pharmacology; D014466:Ultraviolet Rays
D017319:Photosensitizing Agents; D011687:Purines; D011743:Pyrimidines; D017382:Reactive Oxygen Species; D006151:Guanosine; D012395:Rose Bengal; D012313:RNA; D004247:DNA; D003849:Deoxyguanosine; C030986:pyrimidine; D012256:Riboflavin; C030985:purine