Assessment of contamination of the Svitava and Svratka rivers in the Czech Republic using selected biochemical markers.
The aim of the present study is to assess aquatic ecosystem contamination using selected biochemical markers: cytochrome P450, ethoxyresorufin-O-deethylase (EROD), glutathione S-transferase (GST), tripeptide glutathione, vitellogenin, and 11-ketotestosterone in chub (Leuciscus cephalus L.). Seven locations on the Svitava and Svratka rivers (in the Brno conurbation, Czech Republic) were assessed. The results were compared with the levels of the most important inductors of these biomarkers: organic pollutants hexachlorocyclohexane (HCH), hexachlorobenzene (HCB), DDT and its metabolites, polychlorinated biphenyls, and polycyclic aromatic hydrocarbons in bottom sediment, fish muscle, and semipermeable membrane devices (SPMDs) and metals in sediment. The highest levels of pollutants were observed at sites situated downstream from Brno, especially at Modrice and Rajhradice. Significant positive correlations (p < 0.05) were found between EROD activity and HCH concentration in SPMDs, and also between GST and EROD activity with HCB concentration in muscle, after adjusting for age.
Blahová
Jana
J
Department of Veterinary Public Health and Toxicology, University of Veterinary and Pharmaceutical Sciences Brno, Palackého 1-3, Brno, Czech Republic. blahovaj@vfu.cz
Havelková
Marcela
M
Kruzíková
Kamila
K
Hilscherová
Klára
K
Halouzka
Roman
R
Modrá
Helena
H
Grabic
Roman
R
Halírová
Jarmila
J
Jurcíková
Jana
J
Ocelka
Tomás
T
Harustiaková
Danka
D
Svobodová
Zdenka
Z
D000818:Animals; D015415:Biomarkers; D003530:Cyprinidae / Q000378:metabolism*; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism; D018153:Czech Republic; D052244:Endocrine Disruptors / Q000032:analysis; D004784:Environmental Monitoring; D005982:Glutathione Transferase / Q000378:metabolism; D008297:Male; D009930:Organic Chemicals / Q000032:analysis; D045483:Rivers / Q000737:chemistry*; D013739:Testosterone / Q000031:analogs & derivatives / Q000097:blood; D014819:Vitellogenins / Q000097:blood; D014874:Water Pollutants, Chemical / Q000032:analysis*
D015415:Biomarkers; D052244:Endocrine Disruptors; D009930:Organic Chemicals; D014819:Vitellogenins; D014874:Water Pollutants, Chemical; D013739:Testosterone; D019363:Cytochrome P-450 CYP1A1; D005982:Glutathione Transferase; C003600:11-ketotestosterone