title: Molecular cloning and characterization of an achene-seed-specific promoter from motherwort (Leonurus japonicus Houtt).
abstract: LJAMP1 is a small antimicrobial protein purified previously from the seeds of motherwort, and it is expressed preferentially in seeds. A 794-bp upstream sequence of the ATG start codon was isolated using a genome walking method and cloned into the upstream of the β-glucuronidase (GUS) reporter gene to determine the GUS tissue-specific expression pattern. The transgenic tobacco showed that pLJAMP1 promoter derived GUS reporter gene special expression in pollen, achene and seed. The analysis of cis-acting elements also revealed pLJAMP1 promoter contained pollen and seed related transcriptional control elements.
authors.affiliation: Key Laboratory of Eco-Environments in Three Gorges Reservoir Region (Ministry of Education) and the School of Life Science, Southwest University, 400715, Chongqing, China.
authors.forename: Chengjian
authors.initials: C
authors.lastname: Xie
authors.forename: Beibei
authors.initials: B
authors.lastname: Zhang
authors.forename: De
authors.initials: D
authors.lastname: Wang
authors.forename: Fei
authors.initials: F
authors.lastname: Kou
authors.forename: Xupeng
authors.initials: X
authors.lastname: Zhao
authors.forename: Xingyong
authors.initials: X
authors.lastname: Yang
mesh_terms: D019677:Artificial Gene Fusion; D003001:Cloning, Molecular; D017930:Genes, Reporter / Q000235:genetics; D005966:Glucuronidase / Q000096:biosynthesis / Q000235:genetics; D031332:Leonurus / Q000235:genetics*; D011058:Pollen / Q000235:genetics; D011401:Promoter Regions, Genetic; D050436:Regulatory Elements, Transcriptional; D012639:Seeds / Q000235:genetics*; D014026:Nicotiana / Q000235:genetics
chemical_list: D005966:Glucuronidase