title: Effect of taurine chloramine on the production of matrix metalloproteinases (MMPs) in adiponectin- or IL-1beta-stimulated fibroblast-like synoviocytes.
abstract: BACKGROUND
  Adiponectin greatly stimulated the expression of matrix metalloproteinases (MMPs) in fibroblast-like synoviocytes (FLSs) as did IL-1beta. We wondered whether taurine chloramine (TauCl) inhibits the production of MMPs stimulated by adiponectin in the same pattern as by IL-1beta stimulation in vitro
  
  
  METHODS
  Synovial cells from rheumatoid arthritis (RA) patients were treated with adiponectin or interleukin (IL)-1beta for 24 hr in the presence or absence of TauCl. The culture supernatant was collected and the levels of MMPs were measured by enzyme-linked immunosorbent assay (ELISA). The IkappaB signaling pathways stimulated by adiponectin were studied and the levels of NF-kappaB in the nuclei of the cells were analyzed by ELISA.
  
  
  RESULTS
  TauCl (600 microM) inhibited MMP-13, but not MMP-1, expression in IL-1beta-stimulated RA FLSs. However, TauCl at the same concentration significantly inhibited the production of both adiponectin-stimulated MMP-1 and MMP-13 expression. TauCl inhibited the degradation of IkappaB-alpha stimulated by adiponectin, but not by IL-1beta. Similarly, the level of NF-kappaB in the nucleus was increased by adiponectin stimulation and was inhibited by 600 microM TauCl. However, the levels of NF-kappaB increased by IL-1beta stimulation were not inhibited by 600 microM TauCl.
  
  
  CONCLUSIONS
  TauCl more effectively inhibited MMPs expression induced by adiponectin than that by IL-1beta in RA FLS, suggesting that TauCl plays an important role in down-regulating the expression of MMPs in arthritic joints.
authors.affiliation: East-West Bone & Joint Research Center, East-West Neo Medical Center, Kyung Hee University, 149 Sangil-dong, Gangdong-gu, Seoul, 137-727, Republic of Korea. labrea46@yahoo.co.kr
authors.forename: Kyoung Soo
authors.initials: KS
authors.lastname: Kim
authors.forename: Hyun-Mi
authors.initials: HM
authors.lastname: Choi
authors.forename: Da Hee
authors.initials: DH
authors.lastname: Oh
authors.forename: Chaekyun
authors.initials: C
authors.lastname: Kim
authors.forename: Jin Seok
authors.initials: JS
authors.lastname: Jeong
authors.forename: Myung Chul
authors.initials: MC
authors.lastname: Yoo
authors.forename: Hyung-In
authors.initials: HI
authors.lastname: Yang
mesh_terms: D052242:Adiponectin / Q000494:pharmacology*; D001172:Arthritis, Rheumatoid / Q000378:metabolism / Q000473:pathology; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D002478:Cells, Cultured / Q000187:drug effects / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D006801:Humans; D020672:I-kappa B Proteins / Q000378:metabolism; D053583:Interleukin-1beta / Q000494:pharmacology*; D007527:Isoenzymes / Q000096:biosynthesis; D020782:Matrix Metalloproteinases / Q000096:biosynthesis*; D000072000:NF-KappaB Inhibitor alpha; D016328:NF-kappa B / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D013583:Synovial Membrane / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D013654:Taurine / Q000031:analogs & derivatives* / Q000494:pharmacology
chemical_list: D052242:Adiponectin; D004791:Enzyme Inhibitors; D020672:I-kappa B Proteins; D053583:Interleukin-1beta; D007527:Isoenzymes; D016328:NF-kappa B; C000606204:NFKBIA protein, human; D000072000:NF-KappaB Inhibitor alpha; D013654:Taurine; C043410:N-chlorotaurine; D020782:Matrix Metalloproteinases
references.citation: Arthritis Res. 2002;4(2):117-25
references.pmid: 11879547
references.citation: Amino Acids. 2002;23(4):415-8
references.pmid: 12436209
references.citation: Arthritis Res. 2002;4 Suppl 3:S39-49
references.pmid: 12110122
references.citation: Nat Clin Pract Rheumatol. 2006 Sep;2(9):500-10
references.pmid: 16951705
references.citation: Arthritis Res Ther. 2009;11(6):R161
references.pmid: 19883500
references.citation: J Biol Chem. 1996 Jan 19;271(3):1544-50
references.pmid: 8576151
references.citation: J Biol Chem. 1997 Mar 21;272(12):7608-16
references.pmid: 9065415
references.citation: Mol Cell Biol. 1999 Jun;19(6):4431-42
references.pmid: 10330183
references.citation: Nat Rev Immunol. 2006 Mar;6(3):173-82
references.pmid: 16498448
references.citation: Inflamm Res. 2006 Oct;55(10):446-55
references.pmid: 17109072
references.citation: Int Immunopharmacol. 2006 Sep;6(9):1431-40
references.pmid: 16846837
references.citation: Endocrinol Metab Clin North Am. 2008 Sep;37(3):753-68, x-xi
references.pmid: 18775362
references.citation: Clin Endocrinol (Oxf). 2006 Apr;64(4):355-65
references.pmid: 16584505
references.citation: Clin Immunol. 2005 May;115(2):118-28
references.pmid: 15885632
references.citation: J Biol Chem. 2007 Apr 13;282(15):11110-21
references.pmid: 17311929
references.citation: Arthritis Res Ther. 2007;9(4):R80
references.pmid: 17697361
references.citation: J Biol Chem. 1985 Mar 25;260(6):3321-9
references.pmid: 3972827
references.citation: Arthritis Res. 2001;3(1):72-6
references.pmid: 11178129
references.citation: Biochem J. 1997 Apr 1;323 ( Pt 1):13-6
references.pmid: 9173871
references.citation: Arthritis Rheum. 2006 Aug;54(8):2471-80
references.pmid: 16868967
references.citation: Immunopharmacology. 1998 Jul;40(1):27-38
references.pmid: 9776476
references.citation: Genomics. 1997 Mar 1;40(2):222-33
references.pmid: 9119388
references.citation: Arthritis Rheum. 1984 Aug;27(8):857-63
references.pmid: 6466394
references.citation: Free Radic Biol Med. 1993 Dec;15(6):637-43
references.pmid: 8138190
references.citation: Ann N Y Acad Sci. 1999 Nov 18;892:146-54
references.pmid: 10842660
references.citation: N Engl J Med. 1990 May 3;322(18):1277-89
references.pmid: 2271017
references.citation: Arthritis Rheum. 2003 Jun;48(6):1551-5
references.pmid: 12794822
references.citation: J Neuroendocrinol. 2008 Jun;20(6):842-9
references.pmid: 18601708
references.citation: Immunopharmacology. 1996 Sep;34(2-3):89-95
references.pmid: 8886852
references.citation: Ann Rheum Dis. 1981 Aug;40(4):396-9
references.pmid: 7259331
references.citation: Immunol Today. 1997 Jul;18(7):320-4
references.pmid: 9238834
references.citation: Neurochem Res. 2004 Jan;29(1):117-26
references.pmid: 14992270
references.citation: J Biol Chem. 1996 Nov 8;271(45):28509-15
references.pmid: 8910479
references.citation: Front Biosci. 2006 Jan 01;11:529-43
references.pmid: 16146751
references.citation: Annu Rev Immunol. 1996;14:397-440
references.pmid: 8717520
references.citation: Arthritis Res. 2002;4(3):157-64
references.pmid: 12010565
references.citation: Antioxid Redox Signal. 2009 Nov;11(11):2899-937
references.pmid: 19622015
references.citation: Methods Enzymol. 1986;132:569-85
references.pmid: 3821526