title: Basic research on the biology of meta-tetra(hydroxyphenyl) chlorin for photodynamic therapy in gynaecology: Somatic genotoxicity assayed withDrosophila melanogaster.
abstract: The well-established SMART test, a somatic mutation and recombination assay of Drosophila, was applied to assess the possible genotoxicity of sublethal meta-tetra(hydroxyphenyl) chlorin (mTHPC) photodynamic therapy (PDT) to clonogenic cells in situ. The SMART assay monitors the loss of heterozygosity (LOH) at selected cell-marker loci in clonogenic cells of the larval wing primordia. No evidence of genotoxicity was observed under conditions that killed between 38 and 86% of the exposed test larvae. Since the SMART assay is based on the oral uptake of the suspected genotoxic agent, the uptake kinetics of mTHPC by the assay's specific target cells must be known. Therefore, relevant studies are being carried out at present in order to draw final conclusions from this negative test result for genotoxicity.
authors.affiliation: Institute for Medical Radiobiology, University of Zürich and Paul Scherrer Institute, August Forel Str. 7, CH-8029, Zürich, Switzerland.
authors.forename: P M
authors.initials: PM
authors.lastname: Schweizer
authors.forename: H
authors.initials: H
authors.lastname: Walt
authors.forename: M
authors.initials: M
authors.lastname: Fehr
authors.forename: U
authors.initials: U
authors.lastname: Haller
references.citation: Dev Biol. 1971 Jan;24(1):61-87
references.pmid: 5001010
references.citation: Br J Cancer. 1982 Mar;45(3):456-65
references.pmid: 7073939
references.citation: EMBO J. 1987 Oct;6(10):3155-61
references.pmid: 3121306
references.citation: Cancer Res. 1980 Aug;40(8 Pt 1):2915-8
references.pmid: 7388841
references.citation: Cancer Res. 1981 Sep;41(9 Pt 1):3543-5
references.pmid: 7020932
references.citation: Cancer Lett. 1980 Dec;11(2):161-7
references.pmid: 7459844
references.citation: Mutat Res. 1993 May;299(3-4):135-45
references.pmid: 7683082
references.citation: Cancer Res. 1983 Jun;43(6):2622-7
references.pmid: 6850580
references.citation: Annu Rev Genet. 1991;25:281-314
references.pmid: 1687498
references.citation: Mutat Res. 1981 Jun;82(1):23-30
references.pmid: 7022170
references.citation: Arch Gynecol Obstet. 1995;256(4):167-76
references.pmid: 7503588
references.citation: Cancer Res. 1994 Nov 1;54(21):5561-7
references.pmid: 7923197
references.citation: Biochem J. 1987 Jun 15;244(3):711-5
references.pmid: 2965572
references.citation: Int J Radiat Biol. 1995 Mar;67(3):303-13
references.pmid: 7897279
references.citation: Adv Exp Med Biol. 1983;160:151-64
references.pmid: 6220572
references.citation: Mutat Res. 1988 Aug;203(4):297-308
references.pmid: 3136327
references.citation: Environ Mutagen. 1984;6(2):153-88
references.pmid: 6423380
references.citation: Strahlenther Onkol. 1995 Nov;171(11):622-9
references.pmid: 7502225
references.citation: Lasers Surg Med. 1997;20(4):443-50
references.pmid: 9142685
references.citation: Mutagenesis. 1993 Jan;8(1):57-81
references.pmid: 8450769