Polyethylene glycol on stability of chitosan microparticulate carrier for protein.
Stability enhancement of protein-loaded chitosan microparticles under storage was investigated. Chitosan glutamate at 35 kDa and bovine serum albumin as model protein drug were used in this study. The chitosan microparticles were prepared by ionotropic gelation, and polyethylene glycol 200 (PEG 200) was applied after the formation of the particles. All chitosan microparticles were kept at 25°C for 28 days. A comparison was made between those preparations with PEG 200 and without PEG 200. The changes in the physicochemical properties of the microparticles such as size, zeta potential, pH, and percent loading capacity were investigated after 0, 3, 7, 14, and 28 days of storage. It was found that the stability decreased upon storage and the aggregation of microparticles could be observed for both preparations. The reduction in the zeta potential and the increase in the pH, size, and loading capacity were observed when they were kept at a longer period. The significant change of those preparations without PEG 200 was evident after 7 days of storage whereas those with PEG 200 underwent smaller changes with enhanced stability after 28 days of storage. Therefore, this investigation gave valuable information on the stability enhancement of the microparticles. Hence, enhanced stability of chitosan glutamate microparticles for the delivery of protein could be achieved by the application of PEG 200.
Luangtana-Anan
Manee
M
Department of Pharmaceutical Technology, Faculty of Pharmacy, Silpakorn University, Nakhon Pathom, 73000, Thailand. manee@su.ac.th
Limmatvapirat
Sontaya
S
Nunthanid
Jurairat
J
Chalongsuk
Rapeepun
R
Yamamoto
Keiji
K
D002214:Capsules / Q000138:chemical synthesis*; D048271:Chitosan / Q000737:chemistry*; D004058:Diffusion; D004337:Drug Carriers / Q000138:chemical synthesis*; D004339:Drug Compounding / Q000379:methods*; D004355:Drug Stability; D008863:Microspheres; D011092:Polyethylene Glycols / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry*
D002214:Capsules; D004337:Drug Carriers; D011108:Polymers; D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols; D048271:Chitosan
Int J Pharm. 2002 Dec 5;249(1-2):139-47
12433442
Adv Drug Deliv Rev. 2001 Mar 23;47(1):21-37
11251243
Colloids Surf B Biointerfaces. 2007 Sep 1;59(1):24-34
17555948
J Nanosci Nanotechnol. 2006 Sep-Oct;6(9-10):2887-95
17048495
J Pharm Sci. 2002 Jun;91(6):1396-404
12115839
Int J Pharm. 2003 Jan 2;250(1):215-26
12480287
Int J Pharm. 2002 Feb 21;233(1-2):217-25
11897426
Eur J Pharm Biopharm. 2008 May;69(1):31-42
18023160
J Control Release. 2002 Feb 19;79(1-3):29-40
11853916
J Mater Sci Mater Med. 2010 Feb;21(2):597-607
19838772
J Control Release. 2000 May 15;66(2-3):281-92
10742587
J Control Release. 2006 Nov;116(1):1-27
17050027
Int J Pharm. 2006 Oct 31;324(1):67-73
16973314
Int J Pharm. 2007 May 4;336(1):166-73
17145144
J Colloid Interface Sci. 2005 Mar 15;283(2):344-51
15721903
Int J Pharm. 2002 Dec 5;249(1-2):165-74
12433445
Colloids Surf B Biointerfaces. 2005 Aug;44(2-3):65-73
16024239
Pharm Dev Technol. 2005;10(2):189-96
15926667
Int J Pharm. 2005 May 13;295(1-2):235-45
15848008
Biomaterials. 2004 Jun;25(13):2645-55
14751751
Eur J Pharm Biopharm. 2000 Jul;50(1):147-60
10840198
Colloids Surf B Biointerfaces. 2006 Dec 1;53(2):193-202
17049214
Pharm Res. 1998 Sep;15(9):1326-31
9755881
Eur J Pharm Sci. 1998 Apr;6(2):153-63
9795038
J Biol Chem. 1951 Nov;193(1):265-75
14907713