title: Peptidylarginine deiminase type 2 is over expressed in the glaucomatous optic nerve.
abstract: OBJECTIVE
  To determine levels of Peptidyl arginine deiminase 2 (PAD2) and its product protein-bound citrulline in cadaver eyes that suffered from normal tension glaucoma (NTG) compared to primary open angle glaucoma (POAG), and controls.
  
  
  METHODS
  Western analysis, ELISA, and immunohistochemical analysis were performed with human tissues.
  
  
  RESULTS
  We report over expression of PAD2 and higher levels of its product protein-bound citrulline in the optic nerve of normal tension glaucoma patients (NTG).
  
  
  CONCLUSIONS
  This is the first report demonstrating that like in POAG, NTG also possesses elevated levels of both PAD2 and protein-bound citrulline.
authors.affiliation: CIBICI, Faculty of Chemistry, National University of Córdoba, Argentina.
authors.forename: Thamara A
authors.initials: TA
authors.lastname: Cafaro
authors.forename: Stefanía
authors.initials: S
authors.lastname: Santo
authors.forename: Lucena A
authors.initials: LA
authors.lastname: Robles
authors.forename: Nicolás
authors.initials: N
authors.lastname: Crim
authors.forename: Julio A
authors.initials: JA
authors.lastname: Urrets-Zavalia
authors.forename: Horacio M
authors.initials: HM
authors.lastname: Serra
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D002956:Citrulline / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005901:Glaucoma / Q000201:enzymology* / Q000473:pathology*; D006801:Humans; D006867:Hydrolases / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009900:Optic Nerve / Q000201:enzymology* / Q000473:pathology*; D000080064:Protein-Arginine Deiminase Type 2; D000076342:Protein-Arginine Deiminases; D014019:Tissue Donors
chemical_list: D002956:Citrulline; D006867:Hydrolases; C000620352:PADI2 protein, human; D000080064:Protein-Arginine Deiminase Type 2; D000076342:Protein-Arginine Deiminases
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