title: Essential genes for astroglial development and axon pathfinding during zebrafish embryogenesis.
abstract: The formation of the central nervous system depends on the coordinated development of neural and glial cell types that arise from a common precursor. Using an existing group of zebrafish mutants generated by viral insertion, we performed a "shelf-screen" to identify genes necessary for astroglial development and axon scaffold formation. We screened 274 of 315 viral insertion lines using antibodies that label axons (anti-Acetylated Tubulin) and astroglia (anti-Gfap) and identified 25 mutants with defects in gliogenesis, glial patterning, neurogenesis, and axon guidance. We also identified a novel class of mutants affecting radial glial cell numbers. Defects in astroglial patterning were always associated with axon defects, supporting an important role for axon-glial interactions during axon scaffold development. The genes disrupted in these viral lines have all been identified, providing a powerful new resource for the study of axon guidance, glio- and neurogenesis, and neuron-glial interactions during development of the vertebrate CNS.
authors.affiliation: Biological Sciences, Smith College, Northampton, Massachusetts 01063, USA. mbarresi@email.smith.edu
authors.forename: Michael J F
authors.initials: MJ
authors.lastname: Barresi
authors.forename: Sean
authors.initials: S
authors.lastname: Burton
authors.forename: Kristina
authors.initials: K
authors.lastname: Dipietrantonio
authors.forename: Adam
authors.initials: A
authors.lastname: Amsterdam
authors.forename: Nancy
authors.initials: N
authors.lastname: Hopkins
authors.forename: Rolf O
authors.initials: RO
authors.lastname: Karlstrom
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001253:Astrocytes / Q000166:cytology* / Q000378:metabolism*; D001369:Axons / Q000378:metabolism*; D047108:Embryonic Development / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics*; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D029961:Zebrafish Proteins
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