title: Production of adenovirus vectors and their use as a delivery system for influenza vaccines.
abstract: BACKGROUND
  With the emergence of highly pathogenic avian influenza H5N1 viruses that have crossed species barriers and are responsible for lethal infections in humans in many countries, there is an urgent need for the development of effective vaccines which can be produced in large quantities at a short notice and confer broad protection against these H5N1 variants. In order to meet the potential global vaccine demand in a pandemic scenario, new vaccine-production strategies must be explored in addition to the currently used egg-based technology for seasonal influenza.
  
  
  METHODS
  Adenovirus (Ad) based influenza vaccines represent an attractive alternative/supplement to the currently licensed egg-based influenza vaccines. Ad-based vaccines are relatively inexpensive to manufacture, and their production process does not require either chicken eggs or labor-intensive and time-consuming processes necessitating enhanced biosafety facilities. Most importantly, in a pandemic situation, this vaccine strategy could offer a stockpiling option to reduce the response time before a strain-matched vaccine could be developed.
  
  
  RESULTS
  This review discusses Ad-vector technology and the current progress in the development of Ad-based influenza vaccines.
  
  
  CONCLUSIONS
  Ad vector-based influenza vaccines for pandemic preparedness are under development to meet global vaccine demand.
authors.affiliation: Purdue University, Bindley Bioscience Center, School of Veterinary Medicine, Department of Comparative Pathobiology, West Lafayette, IN 47907, USA.
authors.forename: Sai V
authors.initials: SV
authors.lastname: Vemula
authors.forename: Suresh K
authors.initials: SK
authors.lastname: Mittal
mesh_terms: D000256:Adenoviridae / Q000235:genetics* / Q000276:immunology; D000818:Animals; D002959:Civil Defense; D005822:Genetic Vectors / Q000096:biosynthesis* / Q000276:immunology; D006801:Humans; D053124:Influenza A Virus, H5N1 Subtype / Q000235:genetics / Q000276:immunology*; D007252:Influenza Vaccines / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology / Q000600:supply & distribution; D007251:Influenza, Human / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D058873:Pandemics / Q000517:prevention & control*; D019444:Vaccines, DNA / Q000096:biosynthesis
chemical_list: D007252:Influenza Vaccines; D019444:Vaccines, DNA
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