[Construction and identification of blood type B antigen mimetic polypeptide-Mip3beta double expression recombinant plasmid].
OBJECTIVE
To construct and identify blood type B antigen mimetic polypeptide-macrophage inflammatory protein 3beta (Mip3beta) double expression recombinant plasmid.


METHODS
The positive phage clone P1 was obtained using phage random 12-mer peptide library. Specific primers were designed to amplify the phage DNA of P1 and transmembrane domain and inner segment of PBluscript-Fas gene. The products of the amplification were linked into Mip3betav21 to construct blood type B antigen mimetic polypeptide-Mip3beta double expression recombinant plasmid. The recombinant plasmid was transfected into human melanoma cell line B16 to identify its expression.


CONCLUSIONS
Blood type B antigen mimetic polypeptide-Mip3beta double expression recombinant plasmid is successfully obtained and expressed in human melanoma cell line B16.
Luo
Min
M
Oncology Center, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China. coffee911@126.com
Li
Xu-feng
XF
Cen
Dong-zhi
DZ
Zou
Jian-jun
JJ
Zhang
Ji-ren
JR
D001789:Blood Group Antigens / Q000235:genetics*; D045744:Cell Line, Tumor; D015870:Gene Expression; D006801:Humans; D008264:Macrophages; D010455:Peptides / Q000235:genetics*; D010957:Plasmids; D011993:Recombinant Fusion Proteins / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics*
D001789:Blood Group Antigens; D010455:Peptides; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins