title: A monoclonal antibody that specifically binds chitosan in vitro and in situ on fungal cell walls.
abstract: We report the generation of the first monoclonal antibody that specifically binds to the polysaccharide chitosan. Mice were immunized with a mixture of chitosans, and hybridoma clones were screened for specific binders resulting in the isolation of a single clone secreting a chitosan-specific IgM, mAbG7. In ELISAs, the antibody can bind to chitosans of varying composition, but demonstrates the highest affinity for chitosans with lower degrees of acetylation (DA) and very poor binding to chitin. We tested the ability of the antibody to bind to chitosan in situ, using preparations of fungal cell walls. Immunofluorescence microscopy confirmed that the antibody bound strongly to the cell walls of fungi with high levels of chitosan, whereas poor staining was observed in those species with cell walls of predominantly chitin or cellulose. The potential use of this antibody for the detection of fungal contamination and the protection of plants against fungal pathogens is discussed.
authors.affiliation: Fraunhofer Institute for Molecular Biology and Applied Ecology, Forckenbeckstrasse 6, Aachen, Germany.
authors.forename: Max
authors.initials: M
authors.lastname: Schubert
authors.forename: Siham
authors.initials: S
authors.lastname: Agdour
authors.forename: Rainer
authors.initials: R
authors.lastname: Fischer
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Olbrich
authors.forename: Helga
authors.initials: H
authors.lastname: Schinkel
authors.forename: Stefan
authors.initials: S
authors.lastname: Schillberg
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis* / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000032:analysis* / Q000276:immunology; D002473:Cell Wall / Q000737:chemistry* / Q000276:immunology; D048271:Chitosan / Q000032:analysis* / Q000276:immunology; D005658:Fungi / Q000737:chemistry* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D048271:Chitosan