title: A genetic system to assess in vivo the functions of histones and histone modifications in higher eukaryotes.
abstract: Despite the fundamental role of canonical histones in nucleosome structure, there is no experimental system for higher eukaryotes in which basic questions about histone function can be directly addressed. We developed a new genetic tool for Drosophila melanogaster in which the canonical histone complement can be replaced with multiple copies of experimentally modified histone transgenes. This new histone-replacement system provides a well-defined and direct cellular assay system for histone function with which to critically test models in chromatin biology dealing with chromatin assembly, variant histone functions and the biological significance of distinct histone modifications in a multicellular organism.
authors.affiliation: Abteilung für molekulare Entwicklungsbiologie, Max-Planck-Institut für biophysikalische Chemie, Am Fassberg 11, Göttingen 37077, Germany.
authors.forename: Ufuk
authors.initials: U
authors.lastname: Günesdogan
authors.forename: Herbert
authors.initials: H
authors.lastname: Jäckle
authors.forename: Alf
authors.initials: A
authors.lastname: Herzig
mesh_terms: D000818:Animals; D042002:Chromatin Assembly and Disassembly; D004331:Drosophila melanogaster / Q000235:genetics* / Q000254:growth & development*; D005260:Female; D017353:Gene Deletion; D018507:Gene Expression Regulation, Developmental; D006657:Histones / Q000235:genetics / Q000378:metabolism*; D008297:Male; D009707:Nucleosomes / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D019076:Transgenes
chemical_list: D006657:Histones; D009707:Nucleosomes
references.citation: Cell. 2007 Feb 23;128(4):693-705
references.pmid: 17320507
references.citation: Curr Opin Genet Dev. 2009 Apr;19(2):150-8
references.pmid: 19345089
references.citation: Cell. 2008 Sep 19;134(6):1066-78
references.pmid: 18805098
references.citation: Annu Rev Cell Dev Biol. 2005;21:133-53
references.pmid: 16212490
references.citation: Cell. 1990 May 4;61(3):535-47
references.pmid: 2139805
references.citation: Genes Dev. 1998 Dec 1;12(23):3741-51
references.pmid: 9851980
references.citation: Mol Cell Biol. 2002 Apr;22(7):2267-82
references.pmid: 11884612
references.citation: Nat Rev Genet. 2008 Nov;9(11):843-54
references.pmid: 18927579
references.citation: Nature. 1997 Sep 18;389(6648):251-60
references.pmid: 9305837
references.citation: Trends Cell Biol. 2009 Jan;19(1):29-41
references.pmid: 19027300
references.citation: J Cell Sci. 2004 Dec 1;117(Pt 25):6043-51
references.pmid: 15546920
references.citation: Science. 2001 Aug 10;293(5532):1074-80
references.pmid: 11498575
references.citation: Methods Mol Biol. 2008;420:289-302
references.pmid: 18641955
references.citation: J Cell Sci. 2002 Feb 15;115(Pt 4):857-66
references.pmid: 11865041
references.citation: Cold Spring Harb Symp Quant Biol. 1991;56:465-75
references.pmid: 1840259
references.citation: Genetics. 2004 Jun;167(2):797-813
references.pmid: 15238529
references.citation: Proc Natl Acad Sci U S A. 2007 Feb 27;104(9):3312-7
references.pmid: 17360644