Exercise training enhances elastin, fibrillin and nitric oxide in the aorta wall of spontaneously hypertensive rats.
This work aimed to analyze the effect of low-intensity exercise training on ultrastructural and molecular aortic remodeling. Male Wistar-Kyoto rats (WKY) and spontaneously hypertensive rats (SHR) were allocated into four groups: sedentary WKY (SED-WKY), exercised WKY (EX-WKY, 1 h/day, 5 days/week treadmill exercise training), sedentary SHR (SED-SHR), and exercised SHR (EX-SHR). EX-SHR showed blood pressure reduction of 26% in comparison to SED-SHR after 1 month of exercise (P<0.05). At the 20th week, BP level was not different between EX-SHRs and WKYs. Circumferential wall tension (CWT) was higher by 77% in SED-SHRs than in SED-WKYs (P<0.001). Exercise training reduced CWT by 30% in EX- vs. SED-SHR (P<0.001). In SED-SHRs, endothelial cells showed large and numerous cytoplasmatic vacuoles, fragmented inner elastic lamina and scarce elastin and fibrillin, while exercise training ameliorated it in EX-SHR group. The highest eNOS immunodensity was observed in EX-SHR, which was 50% higher than EX-WKY (P<0.01) and 120% higher than SED-SHR (P<0.0001). In conclusion, present findings indicate beneficial effects of exercise training in hypertensive rats since it increased elastin, fibrillin and eNOS content in the aortic wall.
Moraes-Teixeira
Jessica de Andrade
Jde A
Laboratory of Cellular Ultrastructure and Tissue Biology, Biomedical Center, Institute of Biology, State University of Rio de Janeiro, Rio de Janeiro, Brazil.
Félix
Alyne
A
Fernandes-Santos
Caroline
C
Moura
Anibal Sanchez
AS
Mandarim-de-Lacerda
Carlos Alberto
CA
de Carvalho
Jorge Jose
JJ
D000818:Animals; D001011:Aorta / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D001794:Blood Pressure / Q000502:physiology; D004549:Elastin / Q000378:metabolism* / Q000648:ultrastructure; D000071837:Fibrillins; D006973:Hypertension / Q000378:metabolism* / Q000534:rehabilitation; D015151:Immunoblotting; D007150:Immunohistochemistry; D008297:Male; D008840:Microfilament Proteins / Q000378:metabolism* / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D009569:Nitric Oxide / Q000378:metabolism*; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism; D010805:Physical Conditioning, Animal / Q000502:physiology*; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D013718:Tensile Strength
D000071837:Fibrillins; D008840:Microfilament Proteins; D009569:Nitric Oxide; D004549:Elastin; D052250:Nitric Oxide Synthase Type III; C496316:Nos3 protein, rat