title: Y3IP1, a nucleus-encoded thylakoid protein, cooperates with the plastid-encoded Ycf3 protein in photosystem I assembly of tobacco and Arabidopsis.
abstract: The intricate assembly of photosystem I (PSI), a large multiprotein complex in the thylakoid membrane, depends on auxiliary protein factors. One of the essential assembly factors for PSI is encoded by ycf3 (hypothetical chloroplast reading frame number 3) in the chloroplast genome of algae and higher plants. To identify novel factors involved in PSI assembly, we constructed an epitope-tagged version of ycf3 from tobacco (Nicotiana tabacum) and introduced it into the tobacco chloroplast genome by genetic transformation. Immunoaffinity purification of Ycf3 complexes from the transplastomic plants identified a novel nucleus-encoded thylakoid protein, Y3IP1 (for Ycf3-interacting protein 1), that specifically interacts with the Ycf3 protein. Subsequent reverse genetics analysis of Y3IP1 function in tobacco and Arabidopsis thaliana revealed that knockdown of Y3IP1 leads to a specific deficiency in PSI but does not result in loss of Ycf3. Our data indicate that Y3IP1 represents a novel factor for PSI biogenesis that cooperates with the plastid genome-encoded Ycf3 in the assembly of stable PSI units in the thylakoid membrane.
authors.affiliation: Max-Planck-Institut für Molekulare Pflanzenphysiologie, 14476 Potsdam-Golm, Germany.
authors.forename: Christin A
authors.initials: CA
authors.lastname: Albus
authors.forename: Stephanie
authors.initials: S
authors.lastname: Ruf
authors.forename: Mark Aurel
authors.initials: MA
authors.lastname: Schöttler
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Lein
authors.forename: Julia
authors.initials: J
authors.lastname: Kehr
authors.forename: Ralph
authors.initials: R
authors.lastname: Bock
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D002467:Cell Nucleus / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D055785:Gene Knockdown Techniques; D054628:Genome, Chloroplast; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D045331:Photosystem I Protein Complex / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D034622:RNA Interference; D016415:Sequence Alignment; D020524:Thylakoids / Q000378:metabolism*; D014026:Nicotiana / Q000235:genetics* / Q000378:metabolism
chemical_list: D018744:DNA, Plant; D008565:Membrane Proteins; D045331:Photosystem I Protein Complex; D010940:Plant Proteins
references.citation: EMBO J. 1994 Oct 3;13(19):4623-8
references.pmid: 7925303
references.citation: Plant Cell. 2008 Apr;20(4):856-74
references.pmid: 18441214
references.citation: Plant Cell. 2004 Nov;16(11):3084-97
references.pmid: 15494558
references.citation: Biochim Biophys Acta. 1999 Apr 21;1411(1):21-85
references.pmid: 10216153
references.citation: EMBO J. 1996 Sep 16;15(18):5052-9
references.pmid: 8890178
references.citation: RNA. 2009 Jul;15(7):1251-7
references.pmid: 19460869
references.citation: Plant J. 2001 Sep;27(6):581-90
references.pmid: 11576441
references.citation: Biochemistry. 2002 Apr 16;41(15):4872-82
references.pmid: 11939782
references.citation: Structure. 2009 May 13;17(5):637-50
references.pmid: 19446520
references.citation: Plant Cell. 2001 Dec;13(12):2731-45
references.pmid: 11752384
references.citation: Nat Rev Mol Cell Biol. 2004 Dec;5(12):971-82
references.pmid: 15573135
references.citation: Nature. 1970 Aug 15;227(5259):680-5
references.pmid: 5432063
references.citation: J Biol Chem. 2010 Jan 29;285(5):3478-86
references.pmid: 19923216
references.citation: Plant Mol Biol. 1992 Dec;20(6):1203-7
references.pmid: 1463857
references.citation: Plant J. 1998 Dec;16(6):735-43
references.pmid: 10069079
references.citation: J Biol Chem. 2005 Feb 25;280(8):6648-54
references.pmid: 15611066
references.citation: J Chromatogr B Analyt Technol Biomed Life Sci. 2003 Mar 25;786(1-2):45-59
references.pmid: 12651001
references.citation: Photosynth Res. 2004 Feb;79(2):209
references.pmid: 16228395
references.citation: Plant Physiol. 1993 Aug;102(4):1259-67
references.pmid: 8278548
references.citation: Nature. 2001 Jun 21;411(6840):909-17
references.pmid: 11418848
references.citation: Anal Biochem. 1984 Apr;138(1):141-3
references.pmid: 6731838
references.citation: J Biol Chem. 1999 Apr 9;274(15):10466-73
references.pmid: 10187837
references.citation: Biochim Biophys Acta. 1998 Oct 5;1367(1-3):1-62
references.pmid: 9784589
references.citation: Plant Cell. 2004 Apr;16(4):993-1007
references.pmid: 15031412
references.citation: Biochem J. 2001 Jul 1;357(Pt 1):211-6
references.pmid: 11415451
references.citation: Plant Physiol Biochem. 2008 Mar;46(3):228-37
references.pmid: 18272382
references.citation: J Biol Chem. 2002 Jun 7;277(23):20355-66
references.pmid: 11914374
references.citation: Methods Mol Biol. 2009;528:61-70
references.pmid: 19153684
references.citation: Biochimie. 2000 Jun-Jul;82(6-7):615-34
references.pmid: 10946111
references.citation: J Cell Biol. 1997 Oct 6;139(1):95-102
references.pmid: 9314531
references.citation: J Biol Chem. 2002 Apr 19;277(16):14031-9
references.pmid: 11827973
references.citation: Biochem J. 2009 Dec 14;425(1):207-14
references.pmid: 19817716
references.citation: Plant Biotechnol J. 2008 Apr;6(3):246-63
references.pmid: 18086234
references.citation: Nature. 2007 May 3;447(7140):58-63
references.pmid: 17476261
references.citation: EMBO J. 1991 Aug;10(8):2033-40
references.pmid: 1712288
references.citation: Nat Struct Mol Biol. 2008 Feb;15(2):192-8
references.pmid: 18193063
references.citation: Biochem J. 2007 Apr 15;403(2):251-60
references.pmid: 17209805
references.citation: EMBO J. 1987 May;6(5):1155-9
references.pmid: 16453760
references.citation: Proc Natl Acad Sci U S A. 1990 Nov;87(21):8526-30
references.pmid: 11607112
references.citation: J Biol Chem. 2006 Dec 29;281(52):40461-72
references.pmid: 17082184
references.citation: EMBO J. 1997 Oct 15;16(20):6095-104
references.pmid: 9321389
references.citation: Plant Cell. 2009 Aug;21(8):2424-42
references.pmid: 19700633
references.citation: Curr Genet. 1988 Oct;14(4):395-400
references.pmid: 2850871
references.citation: Biochimie. 2000 Jun-Jul;82(6-7):635-45
references.pmid: 10946112
references.citation: J Mol Biol. 2001 Sep 21;312(3):425-38
references.pmid: 11563907
references.citation: J Biol Chem. 2007 Apr 13;282(15):10915-21
references.pmid: 17303568
references.citation: J Biol Chem. 2002 Jun 7;277(23):20343-54
references.pmid: 11914373
references.citation: Plant Physiol. 2006 Jul;141(3):870-8
references.pmid: 16679416
references.citation: Nature. 2003 Dec 11;426(6967):630-5
references.pmid: 14668855
references.citation: Plant J. 2002 Jul;31(2):189-97
references.pmid: 12121448
references.citation: Plant J. 2006 Oct;48(2):296-306
references.pmid: 16984403
references.citation: J Biol Chem. 1997 Mar 7;272(10):6382-7
references.pmid: 9045660
references.citation: Anal Biochem. 1987 Nov 1;166(2):368-79
references.pmid: 2449095
references.citation: Proc Natl Acad Sci U S A. 1984 Apr;81(7):1991-5
references.pmid: 6326095
references.citation: Proc Natl Acad Sci U S A. 1993 Feb 1;90(3):913-7
references.pmid: 8381537
references.citation: Genome Res. 2004 Oct;14(10B):2176-89
references.pmid: 15489341
references.citation: Nat Biotechnol. 1999 Oct;17(10):1030-2
references.pmid: 10504710
references.citation: J Biol Chem. 2004 Jun 4;279(23):24212-7
references.pmid: 15169790
references.citation: J Biol Chem. 2007 Jan 12;282(2):976-85
references.pmid: 17114182
references.citation: Mol Genet Genomics. 2002 Sep;268(1):19-27
references.pmid: 12242495
references.citation: Protist. 2002 Sep;153(3):197-220
references.pmid: 12389811
references.citation: Plant Physiol. 2004 Dec;136(4):4265-74
references.pmid: 15563617