title: Molecular detection of porcine kobuviruses in pigs in Korea and their association with diarrhea.
abstract: Kobuviruses are small, non-enveloped viruses with a single-stranded, positive-sense genomic RNA, belonging to the family Picornaviridae, a highly diverse family of important pathogens of human and other animals. Porcine kobuvirus has been found recently, and consequently, information about the virus is lacking. In this study, we identified porcine kobuviruses from pigs in Korea by RT-PCR, cloning and sequencing, and we showed the existence of genetic diversity among geographically separated porcine kobuviruses through genetic and phylogenetic analysis. Epidemiological studies of porcine kobuvirus linked to diarrhea indicated that porcine kobuvirus infections are endemic in diarrheic pigs in Korea. Statistical analysis of the porcine kobuvirus positive rate between diarrheic and healthy pigs as well as a survey for other enteric pathogens in diarrheic pigs suggests that porcine kobuvirus may play a role as a causative agent of gastroenteritis in pigs.
authors.affiliation: Department of Veterinary Medicine Virology Lab, Seoul National University, Gwanak-gu, Korea.
authors.forename: Seong-Jun
authors.initials: SJ
authors.lastname: Park
authors.forename: Hye-Kwon
authors.initials: HK
authors.lastname: Kim
authors.forename: Hyoung-Joon
authors.initials: HJ
authors.lastname: Moon
authors.forename: Dae-Sub
authors.initials: DS
authors.lastname: Song
authors.forename: Se-Mi
authors.initials: SM
authors.lastname: Rho
authors.forename: Jae-Yeon
authors.initials: JY
authors.lastname: Han
authors.forename: Van-Giap
authors.initials: VG
authors.lastname: Nguyen
authors.forename: Bong-Kyun
authors.initials: BK
authors.lastname: Park
mesh_terms: D000818:Animals; D003967:Diarrhea / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D052699:Kobuvirus / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D010802:Phylogeny; D010850:Picornaviridae Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D056910:Republic of Korea / Q000453:epidemiology; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology / Q000821:virology*