Members of the YjgF/YER057c/UK114 family of proteins inhibit phosphoribosylamine synthesis in vitro.
The YjgF/YER057c/UK114 family of proteins is highly conserved across all three domains of life and currently lacks a consensus biochemical function. Analysis of Salmonella enterica strains lacking yjgF has led to a working model in which YjgF functions to remove potentially toxic secondary products of cellular enzymes. Strains lacking yjgF synthesize the thiamine precursor phosphoribosylamine (PRA) by a TrpD-dependent mechanism that is not present in wild-type strains. Here, PRA synthesis was reconstituted in vitro with anthranilate phosphoribosyltransferase (TrpD), threonine dehydratase (IlvA), threonine, and phosphoribosyl pyrophosphate. TrpD-dependent PRA formation in vitro was inhibited by S. enterica YjgF and the human homolog UK114. Thus, the work herein describes the first biochemical assay for diverse members of the highly conserved YjgF/YER057c/UK114 family of proteins and provides a means to dissect the cellular functions of these proteins.
Lambrecht
Jennifer A
JA
Department of Bacteriology, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.
Browne
Beth Ann
BA
Downs
Diana M
DM
D000877:Anthranilate Phosphoribosyltransferase / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism; D008954:Models, Biological; D010754:Phosphoribosyl Pyrophosphate / Q000235:genetics / Q000378:metabolism; D012260:Ribonucleases / Q000235:genetics / Q000378:metabolism; D012267:Ribosemonophosphates / Q000096:biosynthesis* / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D019779:Salmonella enterica / Q000235:genetics / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D013913:Threonine Dehydratase / Q000235:genetics / Q000378:metabolism
D001426:Bacterial Proteins; C465442:HMF1 protein, S cerevisiae; D006360:Heat-Shock Proteins; D024101:Mitochondrial Proteins; D012267:Ribosemonophosphates; D029701:Saccharomyces cerevisiae Proteins; C008891:phosphoribosylamine; D010754:Phosphoribosyl Pyrophosphate; D000877:Anthranilate Phosphoribosyltransferase; D012260:Ribonucleases; C490788:RIDA protein, human; D013913:Threonine Dehydratase
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