Effects of anticoagulants on human plasma soluble corin levels measured by ELISA.
BACKGROUND
Recently, soluble corin was detected in human plasma. In patients with heart failure, plasma corin levels were lower than that of normal controls. In this study, we analyzed experimental conditions for measuring plasma or serum corin by an immunoassay.


METHODS
Serum and plasma corin levels were measured by ELISA. Effects of different anticoagulants (EDTA, heparin and sodium citrate) on plasma corin levels were examined.


RESULTS
Corin levels in serum were similar to that in plasma with heparin (950±305 vs. 929±301 pg/ml, n=40, p=0.73), but were significantly higher than those in plasma with sodium citrate (735±237 pg/ml, p<0.01) or EDTA (716±261 pg/ml, p<0.001). Native and recombinant human corin proteins were stable in human plasma with EDTA at 4°C or underwent freezing-and-thawing. In 348 healthy Chinese individuals, plasma corin levels ranged from 216 to 1663 pg/ml. The levels were higher in males than that in females (842±283 vs. 569±192 pg/ml, p<0.001).


CONCLUSIONS
Soluble corin was stable in plasma samples. Plasma soluble corin levels vary depending on anticoagulants used. Samples containing heparin had significantly higher levels of corin than that in samples with EDTA or sodium citrate.
Dong
Ningzheng
N
Cyrus Tang Hematology Center, Jiangsu Institute of Hematology, First Affiliated Hospital, Soochow University, Suzhou, China. ningzheng_dong@yahoo.com.cn
Dong
Jing
J
Liu
Peng
P
Xu
Luyao
L
Shi
Sengseng
S
Wu
Qingyu
Q
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000925:Anticoagulants / Q000494:pharmacology*; D016477:Artifacts; D015415:Biomarkers / Q000097:blood; D001774:Blood Chemical Analysis / Q000379:methods* / Q000592:standards; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000592:standards; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011994:Recombinant Proteins / Q000097:blood; D012016:Reference Values; D012697:Serine Endopeptidases / Q000097:blood*; D012995:Solubility; D055815:Young Adult
D000925:Anticoagulants; D015415:Biomarkers; D011994:Recombinant Proteins; C119812:CORIN protein, human; D012697:Serine Endopeptidases
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