Expression of lumican in the articular disc of the human temporomandibular joint.
Lumican belongs to the small leucine-rich repeat proteoglycan (SLRP) gene family and has been reported to exist in the cornea, intervertebral disc and tendon. Lumican plays a significant role in the assembly and regulation of collagen fibres. The human temporomandibular joint (TMJ) disc is made up of fibrocartilage with an extracellular matrix (ECM) composed of collagen and proteoglycans. The existence and behaviour of lumican has not been studied in the human TMJ disc. Therefore, we used immunohistochemical methods to detect lumican, CD34 and vascular endothelial growth factor (VEGF) and histochemical staining with toluidine blue in 13 human TMJ specimens (10 surgically removed and 3 obtained from autopsy). In both the normal and the deformed discs we observed staining with toluidine blue. We found that the area of metachromasia inside the deformed disc was uneven and expression of lumican was strong in the areas negative for metachromasia. Staining of VEGF and CD34 inside the deformed disc was seen. We confirmed the expression of lumican in the human TMJ disc and showed that a large number of fibroblast-like cells existed in the area of strong lumican expression. These new findings about the behaviour of lumican suggest that it may play a key role in the generation of a new collagen network by fibroblast-like cells.
Kiga
N
N
Department of Oral and Maxillofacial Surgery, Wakayama Medical University, 811-1 Kimiidera, Wakayama City, Japan. kibun@wakayama-med.ac.jp
Tojyo
I
I
Matsumoto
T
T
Hiraishi
Y
Y
Shinohara
Y
Y
Fujita
S
S
D000328:Adult; D000368:Aged; D018952:Antigens, CD34 / Q000378:metabolism; D011508:Chondroitin Sulfate Proteoglycans / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D007150:Immunohistochemistry; D007632:Keratan Sulfate / Q000378:metabolism*; D000071234:Lumican; D008297:Male; D008875:Middle Aged; D019224:Temporomandibular Joint Disc / Q000378:metabolism* / Q000473:pathology; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
D018952:Antigens, CD34; D011508:Chondroitin Sulfate Proteoglycans; C000604306:LUM protein, human; D000071234:Lumican; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D007632:Keratan Sulfate
J Biol Chem. 2008 Aug 1;283(31):21305-9
18463092
Arch Oral Biol. 2003 Jan;48(1):55-62
12615142
IUBMB Life. 2008 Dec;60(12):818-23
18949819
J Cell Biol. 1998 Jun 1;141(5):1277-86
9606218
J Cell Biol. 2000 Nov 13;151(4):779-88
11076963
J Biol Chem. 1999 Apr 2;274(14):9636-47
10092650
J Biol Chem. 2001 Apr 13;276(15):12212-21
11152695
Invest Ophthalmol Vis Sci. 2003 May;44(5):2094-102
12714648
Invest Ophthalmol Vis Sci. 1997 May;38(6):1159-67
9152235
Invest Ophthalmol Vis Sci. 2004 Nov;45(11):3849-56
15505028
J Oral Rehabil. 1999 Jul;26(7):600-7
10445480
Blood. 1993 Feb 15;81(4):1001-8
7679004
J Craniofac Surg. 2007 Nov;18(6):1429-33
17993895
FASEB J. 1992 Jun;6(9):2639-45
1612287
Spine (Phila Pa 1976). 1999 Sep 1;24(17):1765-71
10488504
J Biol Chem. 1998 Apr 17;273(16):9615-21
9545293
Osteoarthritis Cartilage. 2002 Oct;10(10):751-7
12359160
Matrix Biol. 2002 Jun;21(4):361-7
12128073
J Rheumatol. 2002 Jan;29(1):34-8
11824968
J Oral Pathol Med. 1999 Aug;28(7):289-92
10432193
J Oral Pathol Med. 2010 Apr;39(4):356-60
19903245
Matrix Biol. 2008 Jul;27(6):526-34
18534835
Tissue Eng. 2007 Jan;13(1):101-10
17518584
J Rheumatol. 1995 Sep;22(9):1624-30
8523334
Biochem J. 1988 Jun 1;252(2):313-23
3046606
Am J Pathol. 2004 Feb;164(2):651-63
14742269
J Biol Chem. 1993 Jun 5;268(16):11874-80
8099356
J Oral Rehabil. 2002 Mar;29(3):282-6
11896846
Dev Dyn. 2006 Sep;235(9):2493-506
16786597
Matrix Biol. 1999 Aug;18(4):381-90
10517185
J Biol Chem. 2000 Jan 28;275(4):2607-12
10644720
J Biol Chem. 1999 Jul 2;274(27):18843-6
10383378
J Biol Chem. 1992 Jan 5;267(1):347-52
1370446
Exp Eye Res. 2004 May;78(5):957-71
15051477
Arch Oral Biol. 2003 Mar;48(3):185-92
12648555
Matrix. 1993 May;13(3):243-54
7687000
Scanning Microsc. 1994;8(2):207-13
7701296
J Biol Chem. 1991 Dec 25;266(36):24773-7
1761572
Int Rev Cytol. 2004;233:1-45
15037361