title: RLK7, a leucine-rich repeat receptor-like kinase, is required for proper germination speed and tolerance to oxidative stress in Arabidopsis thaliana.
abstract: The leucine-rich repeat class of receptor-like kinase (LRR-RLKs) encoding genes represents the largest family of putative receptor genes in the Arabidopsis thaliana genome. However, very little is known about the range of biological process that they control. We present in this paper the functional characterization of RLK7 that has all the structural features of a receptor-like kinase of the plant-specific LRR type. To this end, we identified and characterized three independent T-DNA insertion mutants, constructed lines carrying truncated versions of this putative receptor, one lacking the cytoplasmic kinase domain (RLK7Δkin) and the other one lacking 14 LRR repeats (RLK7ΔLRR) and generated RLK7 overexpressing lines. We thus provide evidences that RLK7 is involved in the control of germination speed and the tolerance to oxidant stress. First, consistent with the expression kinetics of the RLK7 gene in the seeds, we found that all three mutants showed a delay in germination, whereas the overexpressors, RLK7Δkin and RLK7ΔLRR lines displayed a phenotype of more precocious germination. Second, a non-hypothesis driven proteomic approach revealed that in the seedlings of the three T-DNA insertion lines, four enzymes directly or indirectly involved in reactive oxygen species detoxification, were significantly less abundant. Consistent with this finding, the three mutants were less tolerant than the wild type to a hydrogen peroxide treatment, whereas the overexpressors, RLK7Δkin and RLK7ΔLRR lines presented the opposite phenotype.
authors.affiliation: Laboratoire Génome et Développement des Plantes, UMR 5096 CNRS-UPVD-IRD, Université de Perpignan, 58 Avenue Paul Alduy, 66860, Perpignan Cedex, France. delphine.pitorre@univie.ac.at
authors.forename: Delphine
authors.initials: D
authors.lastname: Pitorre
authors.forename: Christel
authors.initials: C
authors.lastname: Llauro
authors.forename: Edouard
authors.initials: E
authors.lastname: Jobet
authors.forename: Jocelyne
authors.initials: J
authors.lastname: Guilleminot
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Brizard
authors.forename: Michel
authors.initials: M
authors.lastname: Delseny
authors.forename: Eric
authors.initials: E
authors.lastname: Lasserre
mesh_terms: D000222:Adaptation, Physiological; D017360:Arabidopsis / Q000201:enzymology / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D018525:Germination; D006861:Hydrogen Peroxide / Q000378:metabolism; D017403:In Situ Hybridization; D007930:Leucine / Q000378:metabolism*; D009154:Mutation; D018384:Oxidative Stress; D011401:Promoter Regions, Genetic; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D029681:Arabidopsis Proteins; D006861:Hydrogen Peroxide; D007930:Leucine
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