title: Quantum-dot-decorated robust transductable bioluminescent nanocapsules.
abstract: Bioluminescence, due to its high sensitivity, has been exploited in various analytical and imaging applications. In this work, we report a highly stable, cell-transductable, and wavelength-tunable bioluminescence system achieved with an elegant and simple design. Using aqueous in situ polymerization on a bioluminescent enzyme anchored with polymerizable vinyl groups, we obtained nanosized core-shell nanocapsules with the enzyme as the core and a cross-linked thin polymer net as the shell. These nanocapsules possess greatly enhanced stability, retained bioactivity, and a readily engineered surface. In particular, by incorporating polymerizable amines in the polymerization, we endowed the nanocapsules with efficient cell-transduction and sufficient conjugation sites for follow-up modification. Following in situ polymerization, decorating the polymer shell with fluorescent quantum dots allowed us to access a continuous tunable wavelength, which extends the application of such bioluminescent nanocapsules, especially in deep tissue. In addition, the unique core-shell structure and adequate conjugation sites on surface enabled us to maximize the BRET efficiency by adjusting the QD/enzyme conjugation ratio.
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, California 90095, USA.
authors.forename: Juanjuan
authors.initials: J
authors.lastname: Du
authors.forename: Changming
authors.initials: C
authors.lastname: Yu
authors.forename: Daocheng
authors.initials: D
authors.lastname: Pan
authors.forename: Jianmin
authors.initials: J
authors.lastname: Li
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.forename: Ming
authors.initials: M
authors.lastname: Yan
authors.forename: Tatiana
authors.initials: T
authors.lastname: Segura
authors.forename: Yunfeng
authors.initials: Y
authors.lastname: Lu
mesh_terms: D006735:Horseradish Peroxidase / Q000737:chemistry / Q000378:metabolism; D008164:Luminescent Proteins / Q000737:chemistry* / Q000378:metabolism; D008958:Models, Molecular; D008968:Molecular Conformation; D053769:Nanocapsules / Q000737:chemistry*; D010539:Permeability; D045663:Quantum Dots
chemical_list: D008164:Luminescent Proteins; D053769:Nanocapsules; D006735:Horseradish Peroxidase
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