title: Application of bioluminescence imaging to therapeutic intervention of herpes simplex virus type I - Thymidine kinase/ganciclovir in glioma.
abstract: Lentiviral vector containing the HSV1-tk and firefly luciferase (fLuc) gene was infected into C6 and C6-TL expressing HSV1-tk and fLuc gene was generated. C6-TL showed higher [(125)I]IVDU uptake than C6. The survival rate of C6-TL decreased more rapidly with increasing GCV dose and was well correlated with fLuc activity. The images of microPET clearly demonstrated higher uptake of [(18)F]FHBG into the C6-TL tumor. Inhibition of tumor growth was observed in C6-TL tumor-bearing mice treated with GCV through tumor size measurement and bioluminescence imaging. The therapeutic effect of HSV1-tk/GCV system can be monitored using bioluminescent imaging and tumor size measurement.
authors.affiliation: Molecular Imaging Research Center, Korea Institute of Radiological and Medical Sciences, Seoul, Republic of Korea.
authors.forename: Su Jin
authors.initials: SJ
authors.lastname: Jang
authors.forename: Joo Hyun
authors.initials: JH
authors.lastname: Kang
authors.forename: Kwang Il
authors.initials: KI
authors.lastname: Kim
authors.forename: Tae Sup
authors.initials: TS
authors.lastname: Lee
authors.forename: Yong Jin
authors.initials: YJ
authors.lastname: Lee
authors.forename: Kyo Chul
authors.initials: KC
authors.lastname: Lee
authors.forename: Kwang Sun
authors.initials: KS
authors.lastname: Woo
authors.forename: Wee Sup
authors.initials: WS
authors.lastname: Chung
authors.forename: Hee Chung
authors.initials: HC
authors.lastname: Kwon
authors.forename: Chun Jeih
authors.initials: CJ
authors.lastname: Ryu
authors.forename: Tae Hyun
authors.initials: TH
authors.lastname: Choi
authors.forename: Chang Woon
authors.initials: CW
authors.lastname: Choi
authors.forename: Sang Moo
authors.initials: SM
authors.lastname: Lim
authors.forename: Gi Jeong
authors.initials: GJ
authors.lastname: Cheon
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001932:Brain Neoplasms / Q000201:enzymology / Q000235:genetics / Q000628:therapy*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005260:Female; D015774:Ganciclovir / Q000378:metabolism / Q000494:pharmacology*; D017930:Genes, Reporter; D041722:Genes, Transgenic, Suicide; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics; D005909:Glioblastoma / Q000201:enzymology / Q000235:genetics / Q000628:therapy*; D018259:Herpesvirus 1, Human / Q000201:enzymology* / Q000235:genetics; D007065:Idoxuridine / Q000031:analogs & derivatives / Q000378:metabolism; D007700:Kinetics; D016086:Lentivirus / Q000235:genetics; D049409:Luciferases, Firefly / Q000096:biosynthesis / Q000235:genetics; D008163:Luminescent Measurements; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D049268:Positron-Emission Tomography; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D013937:Thymidine Kinase / Q000096:biosynthesis* / Q000235:genetics; D047368:Tumor Burden / Q000187:drug effects
chemical_list: D000970:Antineoplastic Agents; D011993:Recombinant Fusion Proteins; C030360:5-(2-iodovinyl)-2'-deoxyuridine; D049409:Luciferases, Firefly; D013937:Thymidine Kinase; D007065:Idoxuridine; D015774:Ganciclovir