Daunomycin interaction with DNA: microcalorimetric studies of the thermodynamics and binding mechanism.
Nucleic acids are an important target for many therapeutics. Small molecules that bind to nucleic acids are important in many aspects of medicines, particularly in cancer chemotherapy. In recent years, many studies have utilized polynucleic acids with various sequences to demonstrate the binding mechanism of daunomycin, a potent anticancer drug. This study describes that isothermal titration calorimetry is a useful tool for studying the fundamental binding mechanism systemically. The results suggest that the binding free energy is more favorable when the temperature is increased. The binding entropy contributes to this effect. Furthermore, the amine group on daunomycin contributes electrostatic interaction that induces the binding process. In addition, enthalpy-entropy compensation is also exhibited in the daunomycin-DNA binding mechanism. This study used an easy, convenient method of performing a systemic study in a recognition system. The results from this study provide additional information about microscopic mechanisms for molecular design and molecular recognition.
Lin
Po-Hsun
PH
Institute of Systems Biology and Bioinformatics, National Central University, Jhong-Li, Taiwan.
Kao
Yung-Hsi
YH
Chang
Yung
Y
Cheng
Yu-Che
YC
Chien
Chih-Cheng
CC
Chen
Wen-Yih
WY
D001709:Biotechnology; D002151:Calorimetry / Q000379:methods*; D004247:DNA / Q000737:chemistry*; D003630:Daunorubicin / Q000737:chemistry*; D013696:Temperature; D013816:Thermodynamics
D004247:DNA; D003630:Daunorubicin