Preparation and evaluation of silica-based ionic liquid-modified stationary phase for HPLC.
A new high-performance liquid chromatography (HPLC) stationary phase was firstly synthesized based on the ionic liquid 2-ethyl-4-methylimidazole chloride. And the performances of the resulting stationary phase were determined by Fourier transform infrared (FT-IR), thermogravimetric analysis (TGA), and (13)C nuclear magnetic resonance (NMR) spectroscopy. The retention characteristics of the resulting stationary phase were evaluated by normal phase HPLC conditions to separate tanshinone I and tanshinone IIA in Danshen herb. The results demonstrated that the synthesized ionic liquid was bond-linked to the silica surface successfully and that IL-modified silica is more effective than unmodified silica when separating tanshinone I and tanshinone IIA in Danshen herb.
Wang
Ye
Y
Center for Advanced Bioseparation Technology, Department of Chemical Engineering, Inha University, Incheon, Korea.
Zhu
Tao
T
Row
Kyung Ho
KH
D045784:Abietanes; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D052578:Ionic Liquids / Q000138:chemical synthesis* / Q000737:chemistry; D019906:Nuclear Magnetic Resonance, Biomolecular; D010616:Phenanthrenes / Q000737:chemistry / Q000302:isolation & purification; D017550:Spectroscopy, Fourier Transform Infrared; D013818:Thermogravimetry
D045784:Abietanes; D052578:Ionic Liquids; D010616:Phenanthrenes; C021751:tanshinone