[Construction of full-length cDNA library of erythrocytic stage Plasmodium vivax].
Blood samples were collected from vivax malaria patients without antimalarial drug therapy. After filtration through Plasmodipur filter to remove white blood cells, Plasmodium vivax-infected RBCs were enriched by Percoll. Total P. vivax in red blood cells was isolated. A full-length cDNA library of erythrocytic stage P. vivax was constructed by the SMART cDNA library construction kit. The volume and recombinant rate of the library were evaluated. The inserted fragments were identified by PCR amplification. The titer of cDNA library was 1.14 x 10(6). The length of inserted fragment ranged from 900 to 2 500 bp, and the recombination efficiency accounted for 97.2%.
Fang
Qiang
Q
Department of Microbiology and Parasitology, Bengbu Medical College; Anhui Key Laboratory of Infection and Immunity, Bengbu 233030, China.
Xia
Hui
H
Yuan
Yuan-Ying
YY
Cao
Jun
J
Wang
Xue-Mei
XM
Qi
Wen-Juan
WJ
Gao
Qi
Q
D003001:Cloning, Molecular; D004912:Erythrocytes / Q000469:parasitology*; D015723:Gene Library; D006801:Humans; D016780:Malaria, Vivax / Q000097:blood / Q000469:parasitology; D010966:Plasmodium vivax / Q000235:genetics* / Q000302:isolation & purification