Molecular characterization and inhibition analysis of the acetylcholinesterase gene from the silkworm maggot, Exorista sorbillans.
Several organophosphorus (OP) insecticides can selectively kill the silkworm maggot, Exorista sorbillans (Es) (Diptera: Tachinidae), while not obviously affecting the host (Bombyx mori) larvae, but the mechanism is not yet clear. In this study, the cDNA encoding an acetylcholinesterase (AChE) from the field Es was isolated. One point mutation (Gly353Ala) was identified. The Es-353G AChE and Es-353A AChE were expressed in baculovirus- insect cell system, respectively. The inhibition results showed that for eserine and Chlorpyrifos, Es-353A AChE was significantly less sensitive than Es-353G AChE. Meanwhile, comparison of the I(50) values of eserine, dichlorvos, Chlorpyrifos and omethoate of recombinant Es AChEs with its host (Bombyx mori) AChEs indicated that, both Es AChEs are more sensitive than B. mori AChEs. The results give an insight of the mechanism that some OP insecticides can selectively kills Es while without distinct effect on its host, B. mori.
Lang
Guo-Jun
GJ
Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, and Institute of Insect Science, Zhejiang University, Hangzhou 310029, China.
Zhang
Ming-Yan
MY
Li
Bao-Ling
BL
Yu
Lin-Lin
LL
Lu
Xing-Meng
XM
Zhang
Chuan-Xi
CX
D000110:Acetylcholinesterase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000818:Animals; D012831:Bombyx / Q000469:parasitology; D002800:Cholinesterase Inhibitors / Q000494:pharmacology; D004175:Diptera / Q000201:enzymology*; D007306:Insecticides / Q000737:chemistry; D008969:Molecular Sequence Data; D017354:Point Mutation; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment
D002800:Cholinesterase Inhibitors; D007306:Insecticides; D011994:Recombinant Proteins; D000110:Acetylcholinesterase