Deficiency of 5-hydroxyisourate hydrolase causes hepatomegaly and hepatocellular carcinoma in mice.
With the notable exception of humans, uric acid is degraded to (S)-allantoin in a biochemical pathway catalyzed by urate oxidase, 5-hydroxyisourate (HIU) hydrolase, and 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase in most vertebrate species. A point mutation in the gene encoding mouse HIU hydrolase, Urah, that perturbed uric acid metabolism within the liver was discovered during a mutagenesis screen in mice. The predicted substitution of cysteine for tyrosine in a conserved helical region of the mutant-encoded HIU hydrolase resulted in undetectable protein expression. Mice homozygous for this mutation developed elevated platelet counts secondary to excess thrombopoietin production and hepatomegaly. The majority of homozygous mutant mice also developed hepatocellular carcinoma, and tumor development was accelerated by exposure to radiation. The development of hepatomegaly and liver tumors in mice lacking Urah suggests that uric acid metabolites may be toxic and that urate oxidase activity without HIU hydrolase function may affect liver growth and transformation. The absence of HIU hydrolase in humans predicts slowed metabolism of HIU after clinical administration of exogenous urate oxidase in conditions of uric acid-related pathology. The data suggest that prolonged urate oxidase therapy should be combined with careful assessment of toxicity associated with extrahepatic production of uric acid metabolites.
Stevenson
William S
WS
Department of Haematology, Royal North Shore Hospital, University of Sydney, St. Leonards, New South Wales 2065, Australia.
Hyland
Craig D
CD
Zhang
Jian-Guo
JG
Morgan
Phillip O
PO
Willson
Tracy A
TA
Gill
Anthony
A
Hilton
Adrienne A
AA
Viney
Elizabeth M
EM
Bahlo
Melanie
M
Masters
Seth L
SL
Hennebry
Sarah
S
Richardson
Samantha J
SJ
Nicola
Nicos A
NA
Metcalf
Donald
D
Hilton
Douglas J
DJ
Roberts
Andrew W
AW
Alexander
Warren S
WS
D000581:Amidohydrolases / Q000737:chemistry / Q000172:deficiency* / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D005260:Female; D016147:Genes, Tumor Suppressor; D022781:Hepatocytes / Q000201:enzymology; D006529:Hepatomegaly / Q000201:enzymology* / Q000209:etiology / Q000235:genetics*; D008114:Liver Neoplasms, Experimental / Q000201:enzymology* / Q000209:etiology / Q000235:genetics*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D008822:Mice, Transgenic; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016296:Mutagenesis; D050505:Mutant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D010641:Phenotype; D017354:Point Mutation; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013922:Thrombocytosis / Q000201:enzymology / Q000235:genetics; D013926:Thrombopoietin / Q000096:biosynthesis; D014503:Urate Oxidase / Q000378:metabolism; D014527:Uric Acid / Q000378:metabolism / Q000633:toxicity
D050505:Mutant Proteins; D012333:RNA, Messenger; D011994:Recombinant Proteins; D014527:Uric Acid; D013926:Thrombopoietin; D014503:Urate Oxidase; D000581:Amidohydrolases; C474249:hydroxyisourate hydrolase
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