Cationic and tissue-specific protein transduction domains identification, characterization, and therapeutic application.
Protein transduction domains (PTDs) are small peptides able to transverse plasma membranes, able to carry proteins, nucleic acid, and viral particles into cells. PTDs can be broadly classified into three types; cationic, hydrophobic, and cell-type specific. The cationic PTDs, comprised of arginines, lysines, and ornithines, and hydrophobic PTDs can efficiently transduce a variety of cell types in culture and in vivo. The tissue-specific transduction domains, identified by screening of peptide display phage libraries for peptides able to confer internalization, have more restricted transduction properties. Here we provide a review of PTDs, focusing on methods for identifying and characterizing both cationic and tissue-specific transduction peptides. In particular, we describe the use of screening peptide phage display libraries to identify tissue-specific transduction peptides.
Zahid
Maliha
M
Department of Microbiology and Molecular Genetics, 450 Technology Drive, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
Lu
Xiaoli
X
Mi
Zhibao
Z
Robbins
Paul D
PD
D000595:Amino Acid Sequence; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D016503:Drug Delivery Systems; D008969:Molecular Sequence Data; D019151:Peptide Library; D010455:Peptides / Q000378:metabolism*; D021381:Protein Transport
D019151:Peptide Library; D010455:Peptides