title: Use of a clinical MRI scanner for preclinical research on rats.
abstract: This study evaluated the feasibility of imaging rat brains using a human whole-body 3-T magnetic resonance imaging (MRI) scanner with specially developed transmit-and-receive radiofrequency coils. The T(1)- and T(2)-weighted images obtained showed reasonable contrast. Acquired contrast-free time-of-flight magnetic resonance angiography images clearly showed the cortical middle cerebral artery (MCA) branches, and interhemispheric differences could be observed. Dynamic susceptibility contrast MRI at 1.17 mm(3) voxel resolution, performed three times following administration of gadolinium diethylenetriamine pentaacetic acid (Gd-DTPA, 0.1 mmol/kg), demonstrated that the arterial input function (AIF) can be obtained from the MCA region, yielding cerebral blood flow (CBF), cerebral blood volume, and mean transit time (MTT) maps. The hypothalamus (HT) to parietal cortex (Pt) CBF ratio was 45.11 +/- 2.85%, and the MTT was 1.29 +/- 0.40 s in the Pt region and 2.32 +/- 0.17 s in the HT region. A single dose of Gd-DTPA enabled the assessment of AIF within MCA territory and of quantitative CBF in rats.
authors.affiliation: Department of Investigative Radiology, Advanced Medical Engineering Center, National Cardiovascular Center Research Institute, Suita, Osaka, Japan.
authors.forename: Akihide
authors.initials: A
authors.lastname: Yamamoto
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sato
authors.forename: Jun-ichiro
authors.initials: J
authors.lastname: Enmi
authors.forename: Kenji
authors.initials: K
authors.lastname: Ishida
authors.forename: Takayuki
authors.initials: T
authors.lastname: Ose
authors.forename: Atsuomi
authors.initials: A
authors.lastname: Kimura
authors.forename: Hideaki
authors.initials: H
authors.lastname: Fujiwara
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Watabe
authors.forename: Takuya
authors.initials: T
authors.lastname: Hayashi
authors.forename: Hidehiro
authors.initials: H
authors.lastname: Iida
mesh_terms: D000818:Animals; D001158:Arteries / Q000502:physiology; D001921:Brain / Q000098:blood supply; D003287:Contrast Media; D005240:Feasibility Studies; D019786:Gadolinium DTPA; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D023421:Models, Animal; D051381:Rats; D051598:Whole Body Imaging
chemical_list: D003287:Contrast Media; D019786:Gadolinium DTPA
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