Evaluation of insecticidal activity of a bacterial strain, Serratia sp. EML-SE1 against diamondback moth.
To identify novel bioinsecticidal agents, a bacterial strain, Serratia sp. EML-SE1, was isolated from a dead larva of the lepidopteran diamondback moth (Plutella xylostella) collected from a cabbage field in Korea. In this study, the insecticidal activity of liquid cultures in Luria-Bertani broth (LBB) and nutrient broth (NB) of a bacterial strain, Serratia sp. EML-SE1 against thirty 3rd and 4th instar larvae of the diamondback moth was investigated on a Chinese cabbage leaf housed in a round plastic cage (Ø 10 x 6 cm). 72 h after spraying the cabbage leaf with LBB and NB cultures containing the bacterial strain, the mortalities of the larvae were determined to be 91.7% and 88.3%, respectively. In addition, the insecticidal activity on potted cabbage containing 14 leaves in a growth cage (165 x 83 x 124 cm) was found to be similar to that of the plastic cage experiment. The results of this study provided valuable information on the insecticidal activity of the liquid culture of a Serratia species against the diamondback moth.
Jeong
Hyung Uk
HU
College of Agriculture & Life Sciences, Chonnam National University, Gwangju, Republic of Korea.
Mun
Hye Yeon
HY
Oh
Hyung Keun
HK
Kim
Seung Bum
SB
Yang
Kwang Yeol
KY
Kim
Iksoo
I
Lee
Hyang Burm
HB
D000818:Animals; D001937:Brassica / Q000254:growth & development / Q000382:microbiology / Q000469:parasitology; D007814:Larva / Q000382:microbiology; D008969:Molecular Sequence Data; D009036:Moths / Q000382:microbiology*; D010572:Pest Control, Biological / Q000379:methods*; D010802:Phylogeny; D010935:Plant Diseases / Q000469:parasitology; D012705:Serratia / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*
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