title: Molecular architecture and assembly of the yeast kinetochore MIND complex.
abstract: The MIND multiprotein complex is a conserved, essential component of eukaryotic kinetochores and is a constituent of the tripartite KMN network that directly attaches the kinetochore to the mitotic spindle. The primary microtubule-binding complex in this network, NDC80, has been extensively characterized, but very little is known about the structure or function of the MIND complex. In this study, we present biochemical, hydrodynamic, electron microscopy, and small-angle x-ray scattering data that provide insight into the overall architecture and assembly of the MIND complex and the physical relationship of the complex with other components of the KMN network. We propose a model for the overall structure of the complex and provide data on the interactions with NDC80, Spc105p, and thus the mitotic spindle.
authors.affiliation: Macromolecular Structure and Function Laboratory, Cancer Research UK London Research Institute, London WC2A 3PX, England, UK.
authors.forename: Daniel P
authors.initials: DP
authors.lastname: Maskell
authors.forename: Xiao-Wen
authors.initials: XW
authors.lastname: Hu
authors.forename: Martin R
authors.initials: MR
authors.lastname: Singleton
mesh_terms: D000595:Amino Acid Sequence; D019281:Dimerization; D004926:Escherichia coli / Q000235:genetics; D056890:Eukaryota; D018386:Kinetochores / Q000378:metabolism*; D008969:Molecular Sequence Data; D046912:Multiprotein Complexes / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure; D010766:Phosphorylation; D011485:Protein Binding / Q000235:genetics; D021122:Protein Subunits / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000302:isolation & purification / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D053838:Scattering, Small Angle; D008941:Spindle Apparatus / Q000235:genetics / Q000378:metabolism; D014961:X-Ray Diffraction; D014965:X-Rays; D015003:Yeasts / Q000235:genetics / Q000378:metabolism
chemical_list: D046912:Multiprotein Complexes; D021122:Protein Subunits; D011993:Recombinant Fusion Proteins
references.citation: J Cell Biol. 2010 Sep 6;190(5):835-52
references.pmid: 20819937
references.citation: Genome Biol. 2006;7(3):R23
references.pmid: 16563186
references.citation: Genes Dev. 2004 Sep 15;18(18):2255-68
references.pmid: 15371340
references.citation: Nat Cell Biol. 2006 Jun;8(6):581-5
references.pmid: 16715078
references.citation: Cell. 2006 Dec 1;127(5):983-97
references.pmid: 17129783
references.citation: Chromosoma. 2001 May;110(2):65-74
references.pmid: 11453556
references.citation: J Mol Biol. 2008 Nov 21;383(4):894-903
references.pmid: 18793650
references.citation: J Biol Chem. 2005 Aug 12;280(32):29088-95
references.pmid: 15961401
references.citation: J Cell Sci. 1997 Jul;110 ( Pt 14):1615-24
references.pmid: 9247195
references.citation: Nat Struct Mol Biol. 2007 Jan;14(1):54-9
references.pmid: 17195848
references.citation: EMBO J. 2003 Dec 15;22(24):6584-97
references.pmid: 14657030
references.citation: Genes Dev. 1999 Jul 1;13(13):1664-77
references.pmid: 10398680
references.citation: EMBO J. 2006 Sep 6;25(17):4033-49
references.pmid: 16932742
references.citation: Dalton Trans. 2004 Apr 21;(8):1189-93
references.pmid: 15252659
references.citation: PLoS One. 2007 May 30;2(5):e478
references.pmid: 17534428
references.citation: Science. 1991 May 24;252(5009):1162-4
references.pmid: 2031185
references.citation: Mol Biol Cell. 2008 Feb;19(2):587-94
references.pmid: 18045986
references.citation: Mol Cell. 2010 May 14;38(3):383-92
references.pmid: 20471944
references.citation: Genes Dev. 2003 Dec 1;17(23):2902-21
references.pmid: 14633972
references.citation: J Cell Biol. 2006 Apr 10;173(1):9-17
references.pmid: 16585270
references.citation: Biophys J. 2000 Mar;78(3):1606-19
references.pmid: 10692345
references.citation: Cell. 2000 Mar 17;100(6):619-33
references.pmid: 10761928
references.citation: Curr Biol. 2009 Apr 28;19(8):694-9
references.pmid: 19345105
references.citation: J Struct Biol. 1999 Dec 1;128(1):82-97
references.pmid: 10600563
references.citation: Cell. 2009 May 15;137(4):672-84
references.pmid: 19450515
references.citation: Cell. 2002 Oct 18;111(2):163-72
references.pmid: 12408861
references.citation: Proc Natl Acad Sci U S A. 2005 Apr 12;102(15):5363-7
references.pmid: 15809444
references.citation: EMBO J. 2009 Sep 2;28(17):2511-31
references.pmid: 19629042
references.citation: Eukaryot Cell. 2002 Apr;1(2):229-40
references.pmid: 12455957
references.citation: J Cell Biol. 2003 Oct 27;163(2):215-22
references.pmid: 14581449
references.citation: Dev Cell. 2007 Nov;13(5):663-676
references.pmid: 17981135
references.citation: Cell. 2008 May 2;133(3):427-39
references.pmid: 18455984
references.citation: Mol Biol Cell. 2005 Feb;16(2):519-31
references.pmid: 15548592
references.citation: Nat Cell Biol. 2006 May;8(5):446-57
references.pmid: 16622420
references.citation: Nat Rev Mol Cell Biol. 2007 May;8(5):379-93
references.pmid: 17426725