In vivo bioprinting for computer- and robotic-assisted medical intervention: preliminary study in mice.
We present the first attempt to apply bioprinting technologies in the perspective of computer-assisted medical interventions. A workstation dedicated to high-throughput biological laser printing has been designed. Nano-hydroxyapatite (n-HA) was printed in the mouse calvaria defect model in vivo. Critical size bone defects were performed in OF-1 male mice calvaria with a 4 mm diameter trephine. Prior to laser printing experiments, the absence of inflammation due to laser irradiation onto mice dura mater was shown by means of magnetic resonance imaging. Procedures for in vivo bioprinting and results obtained using decalcified sections and x-ray microtomography are discussed. Although heterogeneous, these preliminary results demonstrate that in vivo bioprinting is possible. Bioprinting may prove to be helpful in the future for medical robotics and computer-assisted medical interventions.
Keriquel
Virginie
V
INSERM, U577, Bordeaux, F-33076 France and Université Victor Segalen Bordeaux 2, UMR-S577 Bordeaux, F-33076 France.
Guillemot
Fabien
F
Arnault
Isabelle
I
Guillotin
Bertrand
B
Miraux
Sylvain
S
Amédée
Joëlle
J
Fricain
Jean-Christophe
JC
Catros
Sylvain
S
D000818:Animals; D001709:Biotechnology / Q000379:methods*; D018786:Bone Substitutes / Q000627:therapeutic use; D001921:Brain / Q000528:radiation effects; D017076:Computer-Aided Design; D017886:Durapatite / Q000627:therapeutic use; D006651:Histocytochemistry; D008297:Male; D051379:Mice; D053758:Nanoparticles / Q000627:therapeutic use; D012371:Robotics / Q000379:methods*; D012886:Skull / Q000000981:diagnostic imaging / Q000293:injuries; D012887:Skull Fractures / Q000628:therapy*; D013813:Therapy, Computer-Assisted / Q000379:methods*; D023822:Tissue Engineering / Q000379:methods*; D055114:X-Ray Microtomography
D018786:Bone Substitutes; D017886:Durapatite