Rho-associated kinase-dependent contraction of stress fibres and the organization of focal adhesions.
Stress fibres and associated focal adhesions in cells constitute a contractile apparatus that regulates cell motility and contraction. Rho-kinase, an effector molecule of small GTPases, regulates non-muscle cell motility and contractility. Rho-kinase mediates the contraction of stress fibres in a Ca(2+)-independent manner, and is responsible for slower and more finely tuned contraction of stress fibres than that regulated by myosin light chain kinase activity in living cells. The specific inhibition of the Rho-kinase activity causes cells to not only lose their stress fibres and focal adhesions, but also to appear to lose their cytoplasmic tension. Activated Rho-kinase is also involved in the organization of newly formed stress fibres and focal adhesions in living cells.
Katoh
Kazuo
K
Department of Anatomy, Jichi Medical University, 3311-1 Yakushiji, Shimotsuke-city, Tochigi 329-0498, Japan. katoichi@jichi.ac.jp
Kano
Yumiko
Y
Noda
Yasuko
Y
D000818:Animals; D002465:Cell Movement / Q000502:physiology*; D022001:Focal Adhesions / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D022502:Stress Fibers / Q000235:genetics / Q000378:metabolism*; D054460:rho-Associated Kinases / Q000235:genetics / Q000378:metabolism*
D054460:rho-Associated Kinases
J Cell Biol. 1980 Nov;87(2 Pt 1):326-35
7000792
FEBS Lett. 2005 Dec 5;579(29):6611-5
16297917
Cell. 1992 Aug 7;70(3):389-99
1643657
Biochim Biophys Acta. 1998 Sep 16;1404(3):271-81
9739149
J Cell Biol. 2000 Aug 21;150(4):797-806
10953004
J Cell Biol. 1982 Jun;93(3):804-11
6749863
Annu Rev Cell Dev Biol. 1996;12:463-518
8970735
J Cell Sci. 2004 Jul 15;117(Pt 16):3511-8
15226371
Exp Cell Res. 2000 Nov 25;261(1):44-51
11082274
J Cell Biochem. 2008 Mar 1;103(4):1158-70
17661354
Nat Rev Mol Cell Biol. 2003 Jun;4(6):446-56
12778124
Oncogene. 2010 May 6;29(18):2724-38
20208566
FEBS Lett. 1997 Mar 10;404(2-3):118-24
9119047
Exp Cell Res. 1991 Oct;196(2):353-61
1893944
Genes Cells. 2004 Jul;9(7):653-60
15265008
J Cell Physiol. 2007 Oct;213(1):221-8
17476691
J Biol Chem. 1996 Aug 23;271(34):20246-9
8702756
FASEB J. 2010 Jul;24(7):2211-21
20179145
J Cell Biol. 1976 Sep;70(3):541-54
783170
Science. 1996 Jul 12;273(5272):245-8
8662509
EMBO J. 1994 Jun 1;13(11):2600-10
7516876
J Cell Biol. 1999 Nov 29;147(5):1023-38
10579722
Science. 1997 Feb 28;275(5304):1308-11
9036856
J Cell Biol. 1981 Jul;90(1):187-200
7195906
J Cell Biol. 1983 Aug;97(2):416-24
6684121
Genes Cells. 1998 Mar;3(3):177-88
9619630
Science. 1996 Feb 2;271(5249):648-50
8571127
Cancer Res. 2009 May 15;69(10):4167-74
19435897
Annu Rev Cell Biol. 1988;4:487-525
3058164
J Biol Chem. 1995 Dec 8;270(49):29051-4
7493923
J Cell Biol. 1982 Oct;95(1):310-5
6890557
Genes Cells. 2007 May;12(5):623-38
17535253
Mol Cell Biochem. 2004 Apr;259(1-2):197-209
15124925
Methods Enzymol. 2000;325:369-80
11036619
Annu Rev Biochem. 1999;68:459-86
10872457
Mol Biol Cell. 2004 Jul;15(7):3497-508
15133124
Cell Tissue Res. 1976 Feb 27;166(4):427-43
1253242
J Cell Sci. 2010 Mar 1;123(Pt 5):712-22
20144995
Cell. 1980 Nov;22(2 Pt 2):555-61
6893813
J Cell Sci. 2007 Oct 15;120(Pt 20):3491-9
17928305
Nat Rev Mol Cell Biol. 2009 Jan;10(1):21-33
19197329
J Biol Chem. 1999 Dec 24;274(52):37385-90
10601309
Mol Biol Cell. 1998 Jul;9(7):1919-38
9658180
J Biochem. 2009 Feb;145(2):137-50
19008261
Biochem J. 2005 Aug 1;389(Pt 3):763-74
15823093
J Cell Biol. 2004 Feb 2;164(3):427-39
14757754
Science. 2003 Dec 5;302(5651):1704-9
14657486
Biophys J. 2006 May 15;90(10):3762-73
16500961
Proc Natl Acad Sci U S A. 1988 Aug;85(16):5966-70
3413069
Curr Opin Cell Biol. 2005 Oct;17(5):517-23
16099152
J Biol Chem. 1997 May 9;272(19):12257-60
9139666
Cancer Cell. 2005 Sep;8(3):241-54
16169468
J Cell Biol. 2001 Apr 30;153(3):569-84
11331307
Am J Physiol Cell Physiol. 2001 Jun;280(6):C1669-79
11350763
Nature. 1981 Dec 24;294(5843):691-2
7198718
Biochem Biophys Res Commun. 2003 May 30;305(2):223-8
12745062
EMBO J. 1996 May 1;15(9):2208-16
8641286
Cell Struct Funct. 1991 Jun;16(3):231-40
1913854
J Biol Chem. 1997 Oct 3;272(40):25121-7
9312122