Highly sensitive protein detection based on aptamer probe and isothermal nicking enzyme assisted fluorescence signal amplification.
An isothermal and sensitive fluorescence assay for protein detection using aptamer-protein-aptamer conjugates based on nicking enzyme amplification has been developed, which was combined with magnetic microparticles separation, making this assay suitable for protein detection in biological samples.
Xue
Liyun
L
MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, 510631 Guangzhou, China.
Zhou
Xiaoming
X
Xing
Da
D
D052157:Aptamers, Nucleotide; D001483:Base Sequence; D005456:Fluorescent Dyes; D057230:Limit of Detection; D011506:Proteins / Q000032:analysis*; D013050:Spectrometry, Fluorescence
D052157:Aptamers, Nucleotide; D005456:Fluorescent Dyes; D011506:Proteins