title: Integration pattern of HIV-1 based lentiviral vector carrying recombinant coagulation factor VIII in Sk-Hep and 293T cells.
abstract: 293T and Sk-Hep-1 cells were transduced with a replication-defective self-inactivating HIV-1 derived vector carrying FVIII cDNA. The genomic DNA was sequenced to reveal LTR/human genome junctions and integration sites. One hundred and thirty-two sequences matched human sequences, with an identity of at least 98%. The integration sites in 293T-FVIIIDB and in Sk-Hep-FVIIIDB cells were preferentially located in gene regions. The integrations in both cell lines were distant from the CpG islands and from the transcription start sites. A comparison between the two cell lines showed that the lentiviral-transduced DNA had the same preferred regions in the two different cell lines.
authors.affiliation: Department of Clinical, Toxicological and Food Science Analysis, Faculty of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil. elisa@fcfrp.usp.br
authors.forename: Elisa Maria de Sousa
authors.initials: EM
authors.lastname: Russo-Carbolante
authors.forename: Virgínia
authors.initials: V
authors.lastname: Picanço-Castro
authors.forename: Daiani Cristina Cilão
authors.initials: DC
authors.lastname: Alves
authors.forename: Andrielle Castilho
authors.initials: AC
authors.lastname: Fernandes
authors.forename: Graça
authors.initials: G
authors.lastname: Almeida-Porada
authors.forename: Torsten
authors.initials: T
authors.lastname: Tonn
authors.forename: Dimas Tadeu
authors.initials: DT
authors.lastname: Covas
mesh_terms: D002460:Cell Line; D005169:Factor VIII / Q000096:biosynthesis* / Q000235:genetics*; D005822:Genetic Vectors; D015497:HIV-1 / Q000235:genetics / Q000502:physiology*; D022781:Hepatocytes / Q000821:virology; D006801:Humans; D007668:Kidney / Q000821:virology; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D017422:Sequence Analysis, DNA; D016662:Virus Integration
chemical_list: D011994:Recombinant Proteins; C078147:F8 protein, human; D005169:Factor VIII