title: Temperature-dependent requirement for catalase in aerobic growth of Listeria monocytogenes F2365.
abstract: Listeria monocytogenes is a Gram-positive, psychrotrophic, facultative intracellular food-borne pathogen responsible for severe illness (listeriosis). The bacteria can grow in a wide range of temperatures (1 to 45°C), and low-temperature growth contributes to the food safety hazards associated with contamination of ready-to-eat foods with this pathogen. To assess the impact of oxidative stress responses on the ability of L. monocytogenes to grow at low temperatures and to tolerate repeated freeze-thaw stress (cryotolerance), we generated and characterized a catalase-deficient mutant of L. monocytogenes F2365 harboring a mariner-based transposon insertion in the catalase gene (kat). When grown aerobically on blood-free solid medium, the kat mutant exhibited impaired growth, with the extent of impairment increasing with decreasing temperature, and no growth was detected at 4°C. Aerobic growth in liquid was impaired at 4°C, especially under aeration, but not at higher temperatures (10, 25, or 37°C). Genetic complementation of the mutant with the intact kat restored normal growth, confirming that inactivation of this gene was responsible for the growth impairment. In spite of the expected impact of oxidative stress responses on cryotolerance, cryotolerance of the kat mutant was not affected.
authors.affiliation: Department of Food, Bioprocessing and Nutrition Sciences, North Carolina State University, Schaub Hall, Raleigh, NC 27695-7624, USA.
authors.forename: Reha Onur
authors.initials: RO
authors.lastname: Azizoglu
authors.forename: Sophia
authors.initials: S
authors.lastname: Kathariou
mesh_terms: D000332:Aerobiosis / Q000502:physiology; D000818:Animals; D002374:Catalase / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D002642:Chick Embryo / Q000382:microbiology; D003470:Culture Media; D005516:Food Microbiology; D005615:Freezing; D008089:Listeria monocytogenes / Q000201:enzymology / Q000235:genetics / Q000254:growth & development* / Q000502:physiology; D009154:Mutation; D013696:Temperature
chemical_list: D003470:Culture Media; D002374:Catalase
references.citation: PLoS Pathog. 2009 May;5(5):e1000449
references.pmid: 19478867
references.citation: J Dairy Sci. 1980 Jul;63(7):1199-204
references.pmid: 6999043
references.citation: Infect Immun. 2006 Feb;74(2):1323-38
references.pmid: 16428782
references.citation: Appl Environ Microbiol. 2005 Dec;71(12):8314-22
references.pmid: 16332818
references.citation: Appl Environ Microbiol. 2009 Aug;75(16):5315-20
references.pmid: 19542335
references.citation: Microbiology (Reading). 2004 Feb;150(Pt 2):497-512
references.pmid: 14766928
references.citation: J Clin Microbiol. 1998 Sep;36(9):2530-4
references.pmid: 9705387
references.citation: J Bacteriol. 1999 Aug;181(16):5024-32
references.pmid: 10438775
references.citation: Bioinformatics. 2005 Aug 15;21(16):3422-3
references.pmid: 15976072
references.citation: J Food Prot. 2002 Nov;65(11):1811-29
references.pmid: 12430709
references.citation: J Bacteriol. 2002 Aug;184(15):4177-86
references.pmid: 12107135
references.citation: Clin Infect Dis. 2000 Sep;31(3):770-5
references.pmid: 11017828
references.citation: Scand J Immunol. 1993 Apr;37(4):443-6
references.pmid: 8469926
references.citation: Nucleic Acids Res. 2004 Apr 28;32(8):2386-95
references.pmid: 15115801
references.citation: Curr Microbiol. 2009 Feb;58(2):134-8
references.pmid: 18956226
references.citation: Microbiology (Reading). 2002 Jun;148(Pt 6):1855-1862
references.pmid: 12055305
references.citation: J Clin Microbiol. 2006 May;44(5):1917-8
references.pmid: 16672441
references.citation: ILAR J. 2000;41(4):187-96
references.pmid: 11123179
references.citation: Appl Environ Microbiol. 2000 Jul;66(7):3110-2
references.pmid: 10877815
references.citation: Mol Microbiol. 2002 Jul;45(1):9-15
references.pmid: 12100544
references.citation: Eur J Clin Microbiol Infect Dis. 1997 Nov;16(11):839-42
references.pmid: 9447907
references.citation: Infect Immun. 2004 Feb;72(2):1072-83
references.pmid: 14742555
references.citation: Am J Clin Pathol. 1991 Jul;96(1):130-3
references.pmid: 1906240
references.citation: Microbiol Rev. 1991 Sep;55(3):476-511
references.pmid: 1943998
references.citation: Clin Microbiol Rev. 2001 Jul;14(3):584-640
references.pmid: 11432815
references.citation: J Clin Microbiol. 1997 Jan;35(1):179-83
references.pmid: 8968903
references.citation: Infect Immun. 1989 Aug;57(8):2569-73
references.pmid: 2545629
references.citation: Microbes Infect. 2007 Aug;9(10):1236-43
references.pmid: 17720602
references.citation: J Food Prot. 2010 Aug;73(8):1474-9
references.pmid: 20819357
references.citation: Eur J Clin Microbiol Infect Dis. 1996 Dec;15(12):965-7
references.pmid: 9031886
references.citation: J Clin Microbiol. 1978 May;7(5):474-8
references.pmid: 350896
references.citation: Appl Environ Microbiol. 2007 Apr;73(8):2758-61
references.pmid: 17308180
references.citation: Infect Immun. 1979 Mar;23(3):863-72
references.pmid: 110685
references.citation: J Bacteriol. 1991 Aug;173(16):5159-67
references.pmid: 1860824
references.citation: Appl Environ Microbiol. 2008 Mar;74(5):1653-5
references.pmid: 18192405