Nuclear magnetic resonance (NMR) study of the effect of cisplatin on the metabolic profile of MG-63 osteosarcoma cells.
In the present study, (1)H HRMAS NMR spectroscopy was used to assess the changes in the intracellular metabolic profile of MG-63 human osteosarcoma (OS) cells induced by the chemotherapy agent cisplatin (CDDP) at different times of exposure. Multivariate analysis was applied to the cells spectra, enabling consistent variation patterns to be detected and drug-specific metabolic effects to be identified. Statistical recoupling of variables (SRV) analysis and spectral integration enabled the most relevant spectral changes to be evaluated, revealing significant time-dependent alterations in lipids, choline-containing compounds, some amino acids, polyalcohols, and nitrogenated bases. The metabolic relevance of these compounds in the response of MG-63 cells to CDDP treatment is discussed.
Duarte
Iola F
IF
CICECO-Departmento de Química, Campus Universitário de Santiago, Universidade de Aveiro, 3810-193 Aveiro, Portugal.
Lamego
Ines
I
Marques
Joana
J
Marques
M Paula M
MP
Blaise
Benjamin J
BJ
Gil
Ana M
AM
D000438:Alcohols; D000596:Amino Acids; D002794:Choline; D002945:Cisplatin / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics; D008055:Lipids; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D055442:Metabolome / Q000187:drug effects*; D012516:Osteosarcoma / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology
D000438:Alcohols; D000596:Amino Acids; D008055:Lipids; D002794:Choline; D002945:Cisplatin