Construction of stress-induced metabolic pathway from glucose to 1,3-propanediol in Escherichia coli.
1,3-Propanediol is an important chemical widely used in polymer production, but its availability is being restricted owing to its expensive synthesis. The aim of this study was to engineer an Escherichia coli strain that can produce 1,3-propanediol directly from glucose. We successfully constructed a stress-induced metabolic pathway from glucose to 1,3-propanediol in recombinant E. coli by the expression of gpd1 and gpp2 genes from Saccharomyces cerevisiae and dha operon from Klebsiella pneumoniae, respectively. Batch cultivation of the recombinant E. coli showed that 12.1 g/L 1,3-propanediol was accumulated in the culture without using any inducer.
Liang
Quanfeng
Q
State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, People's Republic of China.
Zhang
Haojun
H
Li
Shengnan
S
Qi
Qingsheng
Q
D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005818:Genetic Engineering; D005947:Glucose / Q000378:metabolism*; D006836:Hydro-Lyases / Q000235:genetics / Q000378:metabolism; D007711:Klebsiella pneumoniae / Q000201:enzymology / Q000235:genetics; D053858:Metabolic Networks and Pathways; D009876:Operon; D010744:Phosphoric Monoester Hydrolases / Q000235:genetics / Q000378:metabolism; D011409:Propylene Glycols / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics
D011409:Propylene Glycols; C041787:1,3-propanediol; D010744:Phosphoric Monoester Hydrolases; C040842:glycerol-1-phosphatase; D006836:Hydro-Lyases; C020671:glycerol dehydratase; D005947:Glucose