Label-free imaging, detection, and mass measurement of single viruses by surface plasmon resonance.
We report on label-free imaging, detection, and mass/size measurement of single viral particles in solution by high-resolution surface plasmon resonance microscopy. Diffraction of propagating plasmon waves along a metal surface by the viral particles creates images of the individual particles, which allow us to detect the binding of the viral particles to surfaces functionalized with and without antibodies. We show that the intensity of the particle image is related to the mass of the particle, from which we determine the mass and mass distribution of influenza viral particles with a mass detection limit of approximately 1 ag (or 0.2 fg/mm(2)). This work demonstrates a multiplexed method to measure the masses of individual viral particles and to study the binding activity of the viral particles.
Wang
Shaopeng
S
Center for Bioelectronics and Biosensors, Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA.
Shan
Xiaonan
X
Patel
Urmez
U
Huang
Xinping
X
Lu
Jin
J
Li
Jinghong
J
Tao
Nongjian
N
D053118:Influenza A Virus, H1N1 Subtype / Q000737:chemistry* / Q000302:isolation & purification; D053758:Nanoparticles / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry; D020349:Surface Plasmon Resonance / Q000295:instrumentation / Q000379:methods*; D013499:Surface Properties; D014771:Virion / Q000737:chemistry
D012822:Silicon Dioxide
Phys Med Biol. 2006 Oct 21;51(20):N371-9
17019025
Science. 2007 Aug 10;317(5839):783-7
17615303
Nano Lett. 2006 Apr;6(4):583-6
16608248
Arch Virol. 1981;67(3):203-15
6261719
Biomaterials. 2007 May;28(15):2380-92
17337300
Proc Natl Acad Sci U S A. 2008 Dec 30;105(52):20701-4
19075225
Nano Lett. 2005 Apr;5(4):803-7
15826132
Nat Nanotechnol. 2009 Jul;4(7):445-50
19581898
Anal Chem. 2010 Mar 15;82(6):2480-7
20184350
Anal Chem. 2007 Apr 1;79(7):2979-83
17309232
Chem Rev. 2008 Feb;108(2):462-93
18229953
Proc Natl Acad Sci U S A. 2004 Sep 28;101(39):14017-22
15365183
PLoS Biol. 2007 Jul;5(7):e183
17622193
Angew Chem Int Ed Engl. 2008;47(27):5013-7
18512212
Biophys J. 2004 Jul;87(1):553-61
15240488
Science. 1966 Jun 3;152(3727):1379-81
5937131