title: [Screening and characterization of peptides specifically binding to the schistosomulum tegument of Schistosoma japonicum].
abstract: OBJECTIVE
  To screen and analyze the peptides in 12 phage-display peptide library specifically binding to the schistosomulum tegument of Schistosoma japonicum.
  
  
  METHODS
  A 12 phage-display peptide library was screened with the S. japonicum schistosomula as the target cells for biopanning by degrees, positive clones picked randomly were deduced by DNA sequencing. According the sequence seeing result, immunohistochemical staining was performed to determine the specificity of the phages to the tegument. To test their targeting efficacy, the interested phage clones were infused back to the mice infected with S. japonicum, mice were sacrificed 2.5 hours later, and the phage distribution in the liver and the tegument of schistosomula was appraised, respectively.
  
  
  RESULTS
  After 3 rounds of biopanning, the phage recovery rate increased from 0.77 x 10(-8) to 0.75 x 10(-5), indicating that the phage library was successfully enriched in the tegument of schistosomula. Seventy-five percent (15/20) of the analyzed sequences were identical with a sequence of QHPRIRKOOOOO. The immunohistochemical stainings showed this sequence specifically binding to the tegument. In vivo titering displayed that this sequence selectively targeted the tegument.
  
  
  CONCLUSIONS
  The peptide of QHPRIRKOOOOO specifically binds to the schistosomulum tegument.
authors.affiliation: Department of Immunology, Xiangya Medical School, the Central South University, Changsha 410013, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Wei
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.forename: Qing-Ren
authors.initials: QR
authors.lastname: Zeng
authors.forename: Yu-Xiao
authors.initials: YX
authors.lastname: Chen
authors.forename: Jun
authors.initials: J
authors.lastname: Zhou
authors.forename: Bi-Yuan
authors.initials: BY
authors.lastname: Liu
authors.forename: Yan
authors.initials: Y
authors.lastname: Zou
authors.forename: Sheng-Hui
authors.initials: SH
authors.lastname: Yang
authors.forename: Li-Ting
authors.initials: LT
authors.lastname: Cai
authors.forename: Li-Xin
authors.initials: LX
authors.lastname: Li
authors.forename: Ling-Mei
authors.initials: LM
authors.lastname: Lan
mesh_terms: D000818:Animals; D007814:Larva; D051379:Mice; D008815:Mice, Inbred Strains; D019151:Peptide Library; D010455:Peptides / Q000235:genetics / Q000302:isolation & purification*; D011817:Rabbits; D012549:Schistosoma japonicum / Q000302:isolation & purification*; D017422:Sequence Analysis, DNA
chemical_list: D019151:Peptide Library; D010455:Peptides