Zinc finger-zinc finger interaction between the transcription factors, GATA-1 and Sp1.
In contrast to the extensive understanding of the zinc finger-DNA interactions, less is known about zinc finger-zinc finger interactions. GATA-1 and Sp1 are transcription factors with zinc finger domains for DNA binding. The interaction between the GATA-1 and Sp1 zinc finger domains is important for synergistic transcriptional effects in erythroid genes. Despite the biological importance of the GATA-1 and Sp1 interaction, the molecular mechanism of the interaction remains unclear. We constructed a series of deletion mutants of the zinc finger domains of GATA-1 and Sp1 to identify the regions within the GATA-1 and Sp1 zinc finger domains that interact. The zinc finger-zinc finger interaction modes were also estimated from calorimetric measurements. This revealed that the interaction between the Sp1 and GATA-1 zinc finger domains was primarily electrostatic, and that the linker region of the Sp1 zinc fingers is important for the association with the GATA-1 zinc finger domains. We propose a new molecular mechanism for zinc finger-zinc finger interactions that should contribute to our understanding of the bio-functional role of the interaction between GATA-1 and Sp1.
Imanishi
Miki
M
Institute for Chemical Research, Kyoto University, Uji, Kyoto 611-0011, Japan. imiki@scl.kyoto-u.ac.jp
Imamura
Chika
C
Higashi
Chika
C
Yan
Wei
W
Negi
Shigeru
S
Futaki
Shiroh
S
Sugiura
Yukio
Y
D000595:Amino Acid Sequence; D050982:GATA1 Transcription Factor / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D054730:Protein Interaction Domains and Motifs; D025941:Protein Interaction Mapping; D016329:Sp1 Transcription Factor / Q000235:genetics / Q000378:metabolism*; D055672:Static Electricity; D016335:Zinc Fingers
D050982:GATA1 Transcription Factor; C494688:GATA1 protein, human; D016329:Sp1 Transcription Factor