Enzymatic method to measure β-1,3-β-1,6-glucan content in extracts and formulated products (GEM assay).
An enzymatic method to measure β-glucan content (GEM assay) is applicable in a variety of matrices. The method is composed of swelling the sample with KOH and initial digestion with a lyticase, which is followed by treatment with a mixture of exo-1,3-β-d-glucanase and β-glucosidase that converts the β-glucan to glucose. The glucose generated by the enzymatic hydrolysis is measured by another enzymatic method. The method is shown to be accurate and precise. The method is selective and applicable to both highly branched and unbranched β-1,3-glucans.
Danielson
Michael E
ME
Biothera, 3388 Mike Collins Drive, Eagan, Minnesota 55121, USA.
Dauth
Rosmarie
R
Elmasry
Natalie A
NA
Langeslay
Ryan R
RR
Magee
Andrew S
AS
Will
Paul M
PM
D043326:Glucan 1,3-beta-Glucosidase / Q000378:metabolism; D004693:Glucan Endo-1,3-beta-D-Glucosidase / Q000378:metabolism; D005936:Glucans / Q000032:analysis* / Q000378:metabolism; D005947:Glucose / Q000032:analysis; D005949:Glucose Oxidase; D006878:Hydroxides; D007202:Indicators and Reagents; D009097:Multienzyme Complexes / Q000378:metabolism; D010447:Peptide Hydrolases / Q000378:metabolism; D010544:Peroxidases; D017680:Potassium Compounds; D001617:beta-Glucosidase / Q000378:metabolism
D005936:Glucans; D006878:Hydroxides; D007202:Indicators and Reagents; D009097:Multienzyme Complexes; D017680:Potassium Compounds; C033671:epiglucan; C025310:lyticase; D005949:Glucose Oxidase; D010544:Peroxidases; C023057:glucose peroxidase; D001617:beta-Glucosidase; D004693:Glucan Endo-1,3-beta-D-Glucosidase; D043326:Glucan 1,3-beta-Glucosidase; C117648:beta-1,3-exoglucanase; D010447:Peptide Hydrolases; D005947:Glucose; C029943:potassium hydroxide