title: A nanoparticle dispersion method for in vitro and in vivo nanotoxicity study.
abstract: The dispersion in air of nanoparticles of different sizes, materials and morphologies with controlled agglomeration involving aerosol delivery for in vivo and in vitro studies is one of the most difficult challenges in the field of nanoparticle toxicology. We describe here a nanoparticle dispersion system using an electrospray method to deliver airborne nanoparticles (approximately 10-100 nm) with spatial uniformity and controllable particle concentration for in vitro and in vivo studies. With the dispersion method, single nanoparticles (polystyrene latex particles, TiO(2), Au, Mn, quantum dots, and carbon nanotubes) can be delivered to cells and animals via the air. The degree of agglomeration can be controlled by changing the suspension feeding rate to simulate realistic conditions for exposure studies.
authors.affiliation: Department of Mechanical Engineering, University of Minnesota, 111 Church Street SE, Minneapolis, MN 55455, USA.
authors.forename: Seong C
authors.initials: SC
authors.lastname: Kim
authors.forename: Da-Ren
authors.initials: DR
authors.lastname: Chen
authors.forename: Chaolong
authors.initials: C
authors.lastname: Qi
authors.forename: Robert M
authors.initials: RM
authors.lastname: Gelein
authors.forename: Jacob N
authors.initials: JN
authors.lastname: Finkelstein
authors.forename: Alison
authors.initials: A
authors.lastname: Elder
authors.forename: Karen
authors.initials: K
authors.lastname: Bentley
authors.forename: Günter
authors.initials: G
authors.lastname: Oberdörster
authors.forename: David Y H
authors.initials: DY
authors.lastname: Pui
mesh_terms: D000336:Aerosols / Q000737:chemistry / Q000633:toxicity*; D000818:Animals; D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods; D006801:Humans; D019570:Inhalation Exposure; D053758:Nanoparticles / Q000633:toxicity*; D037742:Nanotubes, Carbon / Q000633:toxicity*; D010316:Particle Size; D051381:Rats
chemical_list: D000336:Aerosols; D037742:Nanotubes, Carbon