Spatial-domain low-coherence quantitative phase microscopy for cancer diagnosis.
A microscopy technique, spatial-domain low-coherence quantitative phase microscopy (SL-QPM) is proposed for speckle-free, quantitative phase imaging of subcellular structures with subnanometer sensitivity. We quantified, for the first time to our knowledge, the refractive index of the cell nuclei on original unmodified histology specimens. We demonstrate that the refractive index of cell nucleus is highly sensitive in detecting cancer, especially in histologically normal-appearing cells from cancer patients. Because this technique is sensitive and does not require special sample processing, it can be disseminated to all clinical settings.
Wang
Pin
P
Department of Medicine, Division of Gastroenterology, Hepatology and Nutrition, University of Pittsburgh, Pittsburgh, Pennsylvania 15232, USA.
Bista
Rajan
R
Bhargava
Rohit
R
Brand
Randall E
RE
Liu
Yang
Y
D006801:Humans; D042541:Intracellular Space / Q000378:metabolism; D008853:Microscopy / Q000379:methods*; D036103:Nanotechnology; D009369:Neoplasms / Q000175:diagnosis* / Q000473:pathology; D055620:Optical Phenomena; D015203:Reproducibility of Results; D012815:Signal Processing, Computer-Assisted
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