title: Phylogenetic relationships among Saccharum clones in Pakistan revealed by RAPD markers.
abstract: Forty sugarcane genotypes (clones), including elite lines, commercial cultivars of Saccharum officinarum and S. barberi clones, were fingerprinted with 30 RAPD markers, using a PCR-based marker assay. The genetic distance for RAPD data was determined according to Nei, and relationships between accessions were graphed in a dendrogram. Genetic distance values ranging from 16.2 to 86.3% were observed among the 40 sugarcane accessions. The lowest genetic distance was found between genotypes US-406 and US-186. These two genotypes differed from each other in only 25 bands with 15 different primers. Genotypes Col-54 and CP-72-2086 were the second most similar group, with a genetic distance of 19.46%. The most dissimilar of all the accessions were CP-77-400 and US-133, with a genetic distance of 86.3%. RAPD fingerprints help sugarcane breeders clarify the genetic pedigree of commercial sugarcane varieties and can be used to evaluate the efficiency of conventional breeding methods.
authors.affiliation: Department of Plant Breeding and Genetics, University of Agriculture, Faisalabad, Pakistan.
authors.forename: S
authors.initials: S
authors.lastname: Nawaz
authors.forename: F A
authors.initials: FA
authors.lastname: Khan
authors.forename: S
authors.initials: S
authors.lastname: Tabasum
authors.forename: M
authors.initials: M
authors.lastname: Zakria
authors.forename: A
authors.initials: A
authors.lastname: Saeed
authors.forename: M Z
authors.initials: MZ
authors.lastname: Iqbal
mesh_terms: D018744:DNA, Plant / Q000235:genetics; D014644:Genetic Variation / Q000235:genetics; D005838:Genotype; D010154:Pakistan; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*; D031786:Saccharum / Q000145:classification / Q000235:genetics*
chemical_list: D018744:DNA, Plant