Determination of cefazedone in human plasma by high performance liquid chromatography-tandem mass spectrometry: Application to a pharmacokinetic study on Chinese volunteers.
A rapid, sensitive and simple high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was developed for determination of cefazedone in human plasma using metronidazole as internal standard (IS). The chromatographic separation was achieved on an Ultimate XB-CN column (2.1mm×150mm, 5μm) with an isocratic mobile phase of acetonitrile and 20mM ammonium acetate in 0.1% formic acid in water (15:85, v/v). Detection was performed using electrospray ionization in positive ion multiple reaction-monitoring mode (SRM), monitoring the transitions m/z 548.2→344.1 for cefazedone and m/z 172.2→128.1 for IS. Calibration curves were linear over a wide range of 0.20-401.12μg/mL for cefazedone in plasma. The lower limit of quantification (LLOQ) was 0.20μg/mL. The intra- and inter-day precisions were less than 7.2%. The average recovery of cefazedone was 90.8-91.0%. The validated method was successfully applied to the pharmacokinetic study of cefazedone in Chinese healthy volunteers following intravenous (IV) administration of 500, 1000 and 2000mg cefazedone injection.
Wu
Dan
D
Department of Pharmacy, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Qian
Zhen-Yu
ZY
Guo
Tingting
T
Tang
Wenyan
W
Xiang
Yi
Y
Zheng
Heng
H
D000085:Acetates; D000097:Acetonitriles; D002437:Cefazolin / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics; D002681:China; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D005260:Female; D006801:Humans; D007275:Injections, Intravenous; D008297:Male; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
D000085:Acetates; D000097:Acetonitriles; C021341:cefazedone; D002437:Cefazolin; C018824:ammonium acetate; C032159:acetonitrile