title: Pseudo-outbreak of adenovirus infection in a neonatal intensive care unit due to a false-positive antigen detection test.
abstract: Twenty-eight of 56 infants in a neonatal intensive care unit had stools positive for adenovirus by the Sure-Vue adenovirus test. Virus cultures of conventionally processed and chloroform-extracted stool samples, as well as conventional and real-time PCR tests, were negative for adenovirus. The cause for the 50% false-positive rate with the antigen test was not determined.
authors.affiliation: Department of Pediatrics, Division of Infectious Diseases, State University of New York at Buffalo School of Medicine and Biomedical Sciences, and the Women and Children’s Hospital of Buffalo, Buffalo, New York 14222, USA. hfaden@upa.chob.edu
authors.forename: Howard
authors.initials: H
authors.lastname: Faden
authors.forename: Rama
authors.initials: R
authors.lastname: Ramani
authors.forename: Daryl
authors.initials: D
authors.lastname: Lamson
authors.forename: Kirsten
authors.initials: K
authors.lastname: St George
mesh_terms: D000256:Adenoviridae / Q000302:isolation & purification*; D000258:Adenovirus Infections, Human / Q000175:diagnosis* / Q000453:epidemiology / Q000821:virology; D000956:Antigens, Viral / Q000032:analysis*; D003428:Cross Infection / Q000175:diagnosis* / Q000453:epidemiology / Q000821:virology; D004196:Disease Outbreaks; D005189:False Positive Reactions; D005243:Feces / Q000821:virology; D005260:Female; D006801:Humans; D007118:Immunoassay / Q000379:methods; D007223:Infant; D015931:Intensive Care, Neonatal; D008297:Male; D016133:Polymerase Chain Reaction; D014773:Virology / Q000379:methods*; D014776:Virus Cultivation
chemical_list: D000956:Antigens, Viral
references.citation: Arch Dis Child. 1993 May;68(5 Spec No):610-1
references.pmid: 8323370
references.citation: J Pediatr. 2005 Apr;146(4):523-7
references.pmid: 15812457
references.citation: Pediatr Infect Dis J. 1992 Jun;11(6):460-5
references.pmid: 1608683
references.citation: Infect Control Hosp Epidemiol. 1988 Sep;9(9):398-404
references.pmid: 2844886
references.citation: J Med Virol. 2003 Jun;70(2):228-39
references.pmid: 12696109
references.citation: Arch Virol. 2007 Jan;152(1):1-9
references.pmid: 16957827