Extraction and in vitro antioxidant activity of intracellular polysaccharide by Pholiota adiposa SX-02.
Response surface methodology (RSM) was used to optimize the extraction parameters for Pholiota adiposa SX-02 intracellular polysaccharide (IPS) produced during submerged culture. The optimum conditions of IPS extraction were predicted to be, ultrasonic power at 564.93 W, precipitation time 30.34 h and pH 8.28, and IPS yield was estimated at 19.75%. The actual value of IPS under these conditions was 20.51%. The in vitro antioxidant results showed that the inhibition effects of IPS at a dosage of 250 mg/l on superoxide anion, hydroxyl and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical were 74.66 ± 5.31%, 69.20 ± 5.13%, and 75.20 ± 6.73%, respectively, which were 27.51 ± 2.23%, 16.58 ± 1.33%, and 9.46 ± 0.72% higher than that of butylated hydroxytoluene (BHT), respectively. The reducing power of IPS was 0.32 ± 0.02 (absorbance at 700 nm), 39.13 ± 3.47% higher than that of BHT. The results provide a reference for large-scale extraction of IPS by P. adiposa SX-02 in industrial fermentation and the IPS can be used as a potential antioxidant which enhances adaptive immune responses.
Deng
Peng
P
College of Life Science, Shandong Agricultural University, Daizong Street 61, Taian, Shandong 271018, PR China.
Zhang
Guquan
G
Zhou
Bo
B
Lin
Rongshan
R
Jia
Le
L
Fan
Keming
K
Liu
Xiaonan
X
Wang
Guoyi
G
Wang
Li
L
Zhang
Jianjun
J
D001713:Biphenyl Compounds / Q000378:metabolism; D003470:Culture Media; D016166:Free Radical Scavengers / Q000302:isolation & purification* / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D017665:Hydroxyl Radical / Q000378:metabolism; D055368:Pholiota / Q000378:metabolism*; D010851:Picrates / Q000378:metabolism; D011134:Polysaccharides / Q000302:isolation & purification* / Q000494:pharmacology
D001713:Biphenyl Compounds; D003470:Culture Media; D016166:Free Radical Scavengers; D010851:Picrates; D011134:Polysaccharides; D017665:Hydroxyl Radical; C004931:1,1-diphenyl-2-picrylhydrazyl