[Effects of ingredients from Chinese herbs with nature of cold or hot on expression of TRPV1 and TRPM8].
OBJECTIVE
To study the effects of the ingredients from Chinese herbs with the nature of cold or hot on the expression of TRPV1 and TRPM8.


METHODS
The effects of ingredients from herbs on primary culture DRG neurons are observed in vitro. The expression quantity of gene is detected by the method of real time PCR. the 2 (-deltadeltaCT) method is applied to analyze the data.


RESULTS
Ingredients from herbs with the nature of cold up-regulate the expression level of TRPV1 and down-regulate that of TRPM8, especially under the temperature condition of 39 degrees C; while ingredients from herbs with the nature of hot up-regulate the expression level of TRPM8 and down-regulated that of TRPV1, which is more significant under the temperature condition of 19 degrees C.


CONCLUSIONS
The regulatory changes of TRPV1 and TRPM8 mRNA expression induced by the chemical ingredients might be related to the cold and hot natures of the herbs from which the ingredients are extracted. And this could be one of the therapeutic mechanisms for the treatment of Chinese herbal medicines to cold- and heat-related diseases.
Sui
Feng
F
Tang Center for Herbal Medicine Research, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Yang
Na
N
Zhang
Changbin
C
Du
Xinliang
X
Li
Lanfang
L
Weng
Xiaogang
X
Guo
Shuying
S
Huo
Hairu
H
Jiang
Tingliang
T
D000818:Animals; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage* / Q000032:analysis; D015870:Gene Expression / Q000187:drug effects*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D050053:TRPM Cation Channels / Q000235:genetics* / Q000378:metabolism; D050916:TRPV Cation Channels / Q000235:genetics* / Q000378:metabolism
D004365:Drugs, Chinese Herbal; D050053:TRPM Cation Channels; D050916:TRPV Cation Channels; C498947:Trpm8 protein, rat; C493922:Trpv1 protein, rat