title: [Detection of bovine, goat, pig and chicken derived ingredients in animal products with universal PCR-microarray method].
abstract: We analyzed the sequence of vertebrate molecular marker genes, then we selected the mitochondrial DNA (mtDNA) 16S rRNA gene as marker gene. In order to detect four kinds of animal-derived ingredients, which including bovine, goat, pig and chicken. We utilized a pair of universal primers, designed four sets of species-specific microarray probes and two pairs of quality control probes. We optimized the PCR amplifications and hybridization conditions, therefore these four kinds of animal-derived ingredients could be rapid and accurate detected by this approach. The detection limits were all reaches 1 pg. We established the detection platform of these four kinds of animal-derived ingredients. This universal PCR-microarray assay provides a new method for the identification of animal-derived ingredients in the import-export field.
authors.affiliation: Faculty of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China.
authors.forename: Fengyun
authors.initials: F
authors.lastname: Shi
authors.forename: Jiankun
authors.initials: J
authors.lastname: Miao
authors.forename: Liping
authors.initials: L
authors.lastname: Zhang
authors.forename: Hong
authors.initials: H
authors.lastname: Tao
authors.forename: Jianqiang
authors.initials: J
authors.lastname: Lü
authors.forename: Zhouxi
authors.initials: Z
authors.lastname: Ruan
authors.forename: Hui
authors.initials: H
authors.lastname: Zong
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D002417:Cattle; D002645:Chickens; D004272:DNA, Mitochondrial / Q000235:genetics*; D005506:Food Contamination / Q000032:analysis*; D006041:Goats; D008460:Meat / Q000032:analysis*; D046228:Microarray Analysis / Q000379:methods*; D008931:Mitochondria, Muscle / Q000201:enzymology / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012680:Sensitivity and Specificity; D013552:Swine
chemical_list: D004272:DNA, Mitochondrial; D012336:RNA, Ribosomal, 16S