title: Interactions between the APOA5 -1131T>C and the FEN1 10154G>T polymorphisms on ω6 polyunsaturated fatty acids in serum phospholipids and coronary artery disease.
abstract: We determined the contribution of the combination of FEN1 10154G>T with the most significant association in the analysis of plasma arachidonic acid (AA, 20:4ω6) and the APOA5-1131T>C on phospholipid ω6PUFA and coronary artery disease (CAD). Patients with CAD (n = 807, 27-81 years of age) and healthy controls (n = 1123) were genotyped for FEN1 10154G>T and APOA5-1131T>C. We found a significant interaction between these two genes for CAD risk (P = 0.007) adjusted for confounding factors. APOA5-1131C allele carriers had a higher CAD risk [odds ratio (OR):1.484, 95% confidence interval (CI):1.31-1.96; P = 0.005] compared with APOA5-1131TT individuals in the FEN1 10154GG genotype group but not in the FEN1 10154T allele group (OR:1.096, 95%CI:0.84-1.43; P = 0.504). Significant interactions between these two genes were also observed for the AA proportion (P = 0.04) and the ratio of AA/linoleic acid (LA, 18:2ω6) (P = 0.004) in serum phospholipids of controls. The APOA5-1131C allele was associated with lower AA (P = 0.027) and AA/LA (P = 0.014) only in controls carrying the FEN1 10154T allele. In conclusion, the interaction between these genes suggests that the FEN1 10154T variant allele decreases AA and AA/LA in the serum phospholipids of carriers of the APOA5-1131C allele, but contributes no significant increase in CAD risk for this population subset despite their increased triglylcerides and decreased apoA5.
authors.affiliation: National Research Laboratory of Clinical Nutrigenetics/Nutrigenomics, Department of Food and Nutrition, College of Human Ecology, Yonsei University, Seoul, Korea.
authors.forename: Ju Yeon
authors.initials: JY
authors.lastname: Park
authors.forename: Jean Kyung
authors.initials: JK
authors.lastname: Paik
authors.forename: Oh Yoen
authors.initials: OY
authors.lastname: Kim
authors.forename: Jey Sook
authors.initials: JS
authors.lastname: Chae
authors.forename: Yangsoo
authors.initials: Y
authors.lastname: Jang
authors.forename: Jong Ho
authors.initials: JH
authors.lastname: Lee
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000072040:Apolipoprotein A-V; D001054:Apolipoproteins A / Q000235:genetics*; D001095:Arachidonic Acids / Q000097:blood; D016022:Case-Control Studies; D003324:Coronary Artery Disease / Q000097:blood* / Q000235:genetics* / Q000378:metabolism; D043371:Fatty Acids, Omega-6 / Q000378:metabolism*; D005260:Female; D045585:Flap Endonucleases / Q000235:genetics*; D006801:Humans; D008054:Lipid Peroxides / Q000097:blood; D008077:Lipoproteins, LDL / Q000097:blood / Q000737:chemistry / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D010316:Particle Size; D010743:Phospholipids / Q000097:blood* / Q000737:chemistry / Q000378:metabolism; D020641:Polymorphism, Single Nucleotide; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: C437704:APOA5 protein, human; D000072040:Apolipoprotein A-V; D001054:Apolipoproteins A; D001095:Arachidonic Acids; D043371:Fatty Acids, Omega-6; D008054:Lipid Peroxides; D008077:Lipoproteins, LDL; D010743:Phospholipids; D014409:Tumor Necrosis Factor-alpha; D045585:Flap Endonucleases
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