The analysis of café espresso using two-dimensional reversed phase-reversed phase high performance liquid chromatography with UV-absorbance and chemiluminescence detection.
In this study, an activity based screening technique combining two-dimensional liquid chromatography (2DHPLC) with UV-absorbance and chemiluminescence detection was applied to study "Ristretto", "Decaffeinatto" and "Volluto" espresso coffees. This technique, which coupled the separation power of 2DHPLC with the sensitivity and selectivity of the chemiluminescence detection, offers great potential for screening complex samples for antioxidant compounds. Detailed information regarding the complexity of the sample, and the variation between these three coffees could be obtained using this multidimensional-hyphenated method of analysis.
Mnatsakanyan
Mariam
M
Australian Centre for Research on Separation Science, Nanoscale Organisation and Dynamics Group, and the School of Natural Sciences, University of Western Sydney, Parramatta, NSW 2050, Australia.
Stevenson
Paul G
PG
Conlan
Xavier A
XA
Francis
Paul S
PS
Goodie
Tiffany A
TA
McDermott
Geoffrey P
GP
Barnett
Neil W
NW
Shalliker
R Andrew
RA
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D057230:Limit of Detection; D049449:Luminescence; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*