Arginine end-functionalized poly(L-lysine) dendrigrafts for the stabilization and controlled release of insulin.
Second generation biodegradable poly(l-lysine) dendrigrafts functionalized with 12-48 arginine end-groups interact, at physiological pH, with insulin affording dendrigraft/insulin complexes as established by dynamic light scattering, ζ-potential, circular dichroism and isothermal titration calorimetry. Binding occurs in two steps; at low dendrigraft/insulin molar ratios (< or = 0.07) interaction is accompanied with the endothermic dissociation of insulin dimers, while at higher molar ratios, complexation of insulin monomers with dendrigraft derivatives occurs exothermically. High levels of insulin complexation efficiencies (>99%) were determined for all derivatives. Stabilization of complexed insulin against enzymatic degradation by trypsin and α-chymotrypsin is observed especially for the highly arginine end-functionalized dendrigrafts. Insulin release rates in simulated intestinal fluid are being controlled by the number of arginine end-groups and released insulin retains its conformation.
Sideratou
Zili
Z
Institute of Physical Chemistry, NCSR Demokritos, 15310 Aghia Paraskevi, Attiki, Greece.
Sterioti
Nikoletta
N
Tsiourvas
Dimitris
D
Tziveleka
Leto-Aikaterini
LA
Thanassoulas
Angelos
A
Nounesis
George
G
Paleos
Constantinos M
CM
D001120:Arginine / Q000737:chemistry*; D050091:Dendrimers / Q000138:chemical synthesis / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007328:Insulin / Q000737:chemistry*; D015394:Molecular Structure; D011107:Polylysine / Q000138:chemical synthesis / Q000737:chemistry*
D050091:Dendrimers; D007328:Insulin; D011107:Polylysine; D001120:Arginine