title: Inhibitory effect of the essential oil from Chamaecyparis obtusa on the growth of food-borne pathogens.
abstract: In this study, the antibacterial activity of essential oil from Chamaecyparis obtusa (Sieb. et Zucc) leaves and twigs was investigated. The test strains were Klebsiella pneumoniae, Listeria monocytogenes, Salmonella typhimurium, Staphylococcus aureus, Escherichia coli, Legionella pneumophila, and Methicilline-resistant Staphylococcus aureus. Antibacterial activity was estimated by measuring bacterial growth inhibition. Histopathological examination was also performed. C. obtusa oil distinctly inhibited the growth of all test strains and exhibited the strongest antibacterial activity against L. monocytogenes. It was chromatographically divided into several fractions. The fractions were further tested against antibacterial activity and their chemical compositions were analyzed. The fraction containing terpinen-4-ol (TA) showed high antibacterial activity toward all strains tested. Tests with authentic samples showed that TA played a major role in the antibacterial activity of C. obtusa oil, and in a mice test, the oil actively minimized inflammation by S. aureus.
authors.affiliation: Division of Green Business Management, Department of Forest Resources Utilization, Korea Forest Research Institute, Seoul, Republic of Korea.
authors.forename: Mi-Jin
authors.initials: MJ
authors.lastname: Park
authors.forename: Won-Sil
authors.initials: WS
authors.lastname: Choi
authors.forename: Ha-Young
authors.initials: HY
authors.lastname: Kang
authors.forename: Ki-Seob
authors.initials: KS
authors.lastname: Gwak
authors.forename: Geun-Shik
authors.initials: GS
authors.lastname: Lee
authors.forename: Eui-Bae
authors.initials: EB
authors.lastname: Jeung
authors.forename: In-Gyu
authors.initials: IG
authors.lastname: Choi
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects / Q000254:growth & development*; D029780:Chamaecyparis / Q000737:chemistry*; D005260:Female; D005516:Food Microbiology; D005517:Foodborne Diseases / Q000188:drug therapy / Q000382:microbiology*; D006131:Growth Inhibitors / Q000494:pharmacology*; D006801:Humans; D051379:Mice; D008813:Mice, Inbred ICR; D009822:Oils, Volatile / Q000032:analysis / Q000494:pharmacology*; D010938:Plant Oils / Q000032:analysis / Q000494:pharmacology*; D013729:Terpenes / Q000032:analysis / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D006131:Growth Inhibitors; D009822:Oils, Volatile; D010938:Plant Oils; D013729:Terpenes; C034019:terpinenol-4
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