New palytoxin-like molecules in Mediterranean Ostreopsis cf. ovata (dinoflagellates) and in Palythoa tuberculosa detected by liquid chromatography-electrospray ionization time-of-flight mass spectrometry.
A rapid, high resolution liquid chromatography coupled with ElectroSpray Ionization Time-Of-Flight Mass Spectrometry (ESI/TOF/MS) method was developed for the determination of the toxin pattern in cultured cells of Ostreopsis cf. ovata from the Mediterranean Sea. The samples were separated on a Phenomenex Luna 3μ HILIC 200A (150 × 2.00 mm) and analyzed by LC/TOF/MS with electrospray ionization (ESI) interface in positive ion mode. The method developed here provides the capability for a fully automated analysis, which requires relatively easy sample preparation and gives clean and simple chromatograms. The method was successfully applied to the determination of ovatoxin-a, mascarenotoxin-a and four new palytoxins in O. cf. ovata. Another new palytoxin was detected in the standard material from Palythoa tuberculosa provided by Wako Chemicals.
Rossi
Rachele
R
Istituto Zooprofilattico Sperimentale del Mezzogiorno, Via Salute 2, 80055 Portici, Italy.
Castellano
Vincenzo
V
Scalco
Eleonora
E
Serpe
Luigi
L
Zingone
Adriana
A
Soprano
Vittorio
V
D000178:Acrylamides / Q000737:chemistry* / Q000302:isolation & purification; D056893:Alveolata / Q000737:chemistry*; D000818:Animals; D037421:Anthozoa / Q000737:chemistry*; D002478:Cells, Cultured; D002853:Chromatography, Liquid; D003064:Cnidarian Venoms / Q000737:chemistry* / Q000302:isolation & purification; D004141:Dinoflagellida / Q000737:chemistry*; D008522:Mediterranean Sea; D021241:Spectrometry, Mass, Electrospray Ionization; D000095702:Polyether Toxins
D000178:Acrylamides; D003064:Cnidarian Venoms; C010272:palytoxin; D000095702:Polyether Toxins