Improved method for bacterial cell capture after flow cytometry cell sorting.
Fixed cells with different nucleic acid contents and scatter properties (low nucleic acid [LNA], high nucleic acid 1 [HNA1], and HNA2) were sorted by flow cytometry (FCM). For each sort, 10,000 cells were efficiently captured on poly-l-lysine-coated microplates, resulting in efficient and reproducible PCR amplification.
Guillebault
D
D
UPMC Université Paris 06, UMR 7621, LOMIC, Observatoire Océanologique, F-66651 Banyuls/mer, France.
Laghdass
M
M
Catala
P
P
Obernosterer
I
I
Lebaron
P
P
D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification*; D002469:Cell Separation / Q000379:methods*; D005434:Flow Cytometry / Q000379:methods*; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques / Q000379:methods; D009696:Nucleic Acids / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology
D009696:Nucleic Acids; D012336:RNA, Ribosomal, 16S
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