The role of an E-box element: multiple frunctions and interacting partners.
Circadian clocks can be entrained by light-dark or temperature cycles. In the green alga Chlamydomonas reinhardtii, 12h changes in temperature between 18°C and 28°C synchronize its clock. Both subunits of the circadian RNA-binding protein CHLAMY1, named C1 and C3, are able to integrate temperature information. C1 gets hyper-phosphorylated in cells grown at 18°C and the level of C3 is up-regulated at this temperature. In the long period mutant per1, where temperature entrainment is disturbed, the temperature-dependent regulation of C1 and C3 is altered. Up-regulation of C3 at the low temperature is mediated predominantly by an E-box element situated in its promoter region. This cis-acting element is also relevant for circadian expression of c3 as well as of its up-regulation in cells, where C1 is overexpressed. Among the few identified factors interacting with the E-box region, C3 is also present, suggesting that it feedbacks on its own transcription.
Seitz
Stefanie B
SB
Institut für Allgemeine Botanik und Pflanzenphysiologie, Friedrich-Schiller-Universität Jena, Jena, Germany.
Voytsekh
Olga
O
Mohan
Karthik M
KM
Mittag
Maria
M
D016825:Chlamydomonas reinhardtii / Q000378:metabolism / Q000502:physiology*; D002940:Circadian Rhythm / Q000502:physiology*; D024721:E-Box Elements / Q000502:physiology*; D025461:Feedback, Physiological; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D021122:Protein Subunits / Q000235:genetics* / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D013696:Temperature; D015854:Up-Regulation
D010940:Plant Proteins; D021122:Protein Subunits; D016601:RNA-Binding Proteins
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