Role of the RNA/DNA kinase Grc3 in transcription termination by RNA polymerase I.
Transcription termination by RNA polymerase I in Saccharomyces cerevisiae is mediated by a 'torpedo' mechanism: co-transcriptional RNA cleavage by Rnt1 at the ribosomal DNA 3'-region generates a 5'-end that is recognized by the 5'-3' exonuclease Rat1; this degrades the downstream transcript and eventually causes termination. In this study, we identify Grc3 as a new factor involved in this process. We demonstrate that GRC3, an essential gene of previously unknown function, encodes a polynucleotide kinase that is required for efficient termination by RNA polymerase I. We propose that it controls the phosphorylation status of the downstream Rnt1 cleavage product and thereby regulates its accessibility to the torpedo Rat1.
Braglia
Priscilla
P
Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK.
Heindl
Katrin
K
Schleiffer
Alexander
A
Martinez
Javier
J
Proudfoot
Nick J
NJ
D004256:DNA Polymerase I / Q000235:genetics / Q000378:metabolism; D004275:DNA, Ribosomal / Q000235:genetics / Q000378:metabolism; D010766:Phosphorylation; D011116:Polynucleotide 5'-Hydroxyl-Kinase / Q000235:genetics / Q000378:metabolism*; D012318:RNA Polymerase I / Q000378:metabolism*; D012323:RNA Processing, Post-Transcriptional; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D013728:Terminator Regions, Genetic; D014158:Transcription, Genetic
D004275:DNA, Ribosomal; D029701:Saccharomyces cerevisiae Proteins; D011116:Polynucleotide 5'-Hydroxyl-Kinase; D012318:RNA Polymerase I; D004256:DNA Polymerase I
RNA. 2009 May;15(5):923-31
19299550
Genes Dev. 2008 Apr 15;22(8):1082-92
18413718
Nucleic Acids Res. 2007;35(1):87-99
17151076
Nat Struct Mol Biol. 2007 Feb;14(2):123-30
17259992
Proc Natl Acad Sci U S A. 1997 Jul 22;94(15):7897-902
9223284
Mol Cell Biol. 2003 Mar;23(6):2042-54
12612077
Nature. 2004 Nov 25;432(7016):522-5
15565158
Mol Cell Biol. 2000 Jun;20(11):4006-15
10805743
Mol Microbiol. 2008 Jan;67(1):143-54
18047582
Cell. 2003 Jun 27;113(7):919-33
12837249
Mol Cell Biol. 1993 Jan;13(1):341-50
8417335
Yeast. 2000 Feb;16(3):277-88
10649456
Nucleic Acids Res. 1997 Sep 1;25(17):3389-402
9254694
RNA. 2008 Sep;14(9):1737-45
18648070
EMBO J. 2000 Nov 1;19(21):5895-904
11060040
Nature. 2007 May 10;447(7141):222-6
17495927
Cell. 1994 Nov 4;79(3):527-34
7954818
Genes Dev. 2008 Apr 15;22(8):1069-81
18413717
J Biol Chem. 1980 Apr 10;255(7):3080-5
6244307
RNA. 1999 Jul;5(7):909-17
10411134
Nature. 2009 Apr 9;458(7239):784-8
19194460
BMC Bioinformatics. 2004 Aug 19;5:113
15318951
Nature. 2004 Nov 25;432(7016):517-22
15565157