Hyperphosphorylated neurofilament NF-H as a biomarker of the efficacy of minocycline therapy for spinal cord injury.
METHODS
An in vivo study in a rat model of acute spinal cord contusion.


OBJECTIVE
To assess the efficacy of novel therapies for acute spinal cord injury (SCI), methods to evaluate accurately the effects of these therapies should be developed. Although neurological examination is commonly used for this purpose, unstable clinical conditions and the spontaneous recovery of neurological function in the acute and subacute phases after injury make this measurement unreliable. Recent studies have reported that the phosphorylated form of the high-molecular-weight neurofilament subunit NF-H (pNF-H), a new biomarker for axonal degeneration, can be measured in serum samples in experimental SCI animals. Therefore, we aimed to investigate the use of plasma pNF-H as an indicator of the efficacy of minocycline, a neuroprotective drug, for treating SCI.


METHODS
This study was carried out at Saitama, Japan.


METHODS
Spinal cord injured rats received either minocycline or saline intraperitoneally. The plasma pNF-H levels and functional hind limb score were determined after the injury.


RESULTS
Minocycline treatment reduced plasma pNF-H levels at 3 and 4 days post-injury (dpi). Rats with lower plasma pNF-H levels at 3 dpi had higher hind limb motor score at 28 dpi.


CONCLUSIONS
pNF-H levels may serve as a biomarker for evaluating the efficacy of therapies for SCI.
Ueno
T
T
Department of Rehabilitation for the Movement Functions, Research Institute, National Rehabilitation Center for Persons with Disabilities, Tokorozawa, Saitama, Japan.
Ohori
Y
Y
Ito
J
J
Hoshikawa
S
S
Yamamoto
S
S
Nakamura
K
K
Tanaka
S
S
Akai
M
M
Tobimatsu
Y
Y
Ogata
T
T
D000818:Animals; D015415:Biomarkers / Q000097:blood; D004195:Disease Models, Animal; D008911:Minocycline / Q000494:pharmacology / Q000627:therapeutic use*; D016900:Neurofilament Proteins / Q000097:blood*; D018696:Neuroprotective Agents / Q000627:therapeutic use*; D010766:Phosphorylation / Q000187:drug effects / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function / Q000187:drug effects / Q000502:physiology; D013119:Spinal Cord Injuries / Q000188:drug therapy* / Q000378:metabolism / Q000503:physiopathology
D015415:Biomarkers; D016900:Neurofilament Proteins; D018696:Neuroprotective Agents; C051774:neurofilament protein H; D008911:Minocycline