title: Structural studies of MAP Kinase cascade components.
abstract: MAPK cascade components have been the subject of structural analysis, advancing our understanding of how these enzymes are activated and how they interact. A surprising finding has been that unique inactive conformers are adopted by many of these kinases. These inactive conformers are interesting and often require experimental phases to determine their crystal structures because molecular replacement techniques are not successful. Here, we describe the preparation of MAP2K MEK6 and MAP3K TAO2 substituted with selenomethionine (SeMet) for de novo phasing. TAO2 and SeMet TAO2 were expressed in insect cells.
authors.affiliation: Department of Biochemistry, The University of Texas Southwestern Medical Center at Dallas, Dallas, TX, USA. Elizabeth.Goldsmith@UTSouthwestern.edu
authors.forename: Elizabeth J
authors.initials: EJ
authors.lastname: Goldsmith
authors.forename: Xiaoshan
authors.initials: X
authors.lastname: Min
authors.forename: Haixia
authors.initials: H
authors.lastname: He
authors.forename: Tianjun
authors.initials: T
authors.lastname: Zhou
mesh_terms: D000818:Animals; D002460:Cell Line; D003460:Crystallization; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D048669:MAP Kinase Kinase 6 / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D020930:MAP Kinase Kinase Kinases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D020935:MAP Kinase Signaling System; D051379:Mice; D010766:Phosphorylation; D051381:Rats; D012645:Selenomethionine / Q000378:metabolism
chemical_list: D012645:Selenomethionine; D020930:MAP Kinase Kinase Kinases; D048669:MAP Kinase Kinase 6