Differential expression profiles of Alternaria alternate genes in response to carbonyl sulfide fumigation.
Carbonyl sulfide (COS) is a new fumigant used in phytosanitary treatments. It was developed as a potential alternative to methyl bromide, which is being phased out because of its ozone-depletion properties. To understand the molecular and cellular mechanisms occurring in fungal pathogens in response to COS fumigation, we cloned 510 cDNA fragments of Alternaria alternata (Fr.) Keissler genes that are differentially expressed; these genes were cloned using suppression subtractive hybridization. Changes in the levels of transcripts of 79 fragments were confirmed by microarray analysis and qRT-PCR. Further homology search revealed that they are highly homologous to 41 genes of other fungi, which were related to general metabolism, growth and division, defense, cellular transport, and signal transduction. These results provide an overview of differential expression profiles of A. alternata genes following COS treatment and some new clues about the mechanism of COS fungitoxicity.
Liu
Tao
T
Chinese Academy of Inspection and Quarantine, NO.241, Huixinxijie, Chaoyang district, Beijing, P. R. China.
Li
Li
L
Wang
Yuejin
Y
Zhan
Guoping
G
Liu
Bo
B
D000528:Alternaria / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D005651:Fumigation; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D005659:Fungicides, Industrial / Q000494:pharmacology*; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects*; D010935:Plant Diseases / Q000382:microbiology; D031989:Pyrus / Q000382:microbiology; D013461:Sulfur Oxides / Q000494:pharmacology*
D005656:Fungal Proteins; D005659:Fungicides, Industrial; D013461:Sulfur Oxides; C010063:carbonyl sulfide
Biochem Pharmacol. 2003 Oct 15;66(8):1499-503
14555227
Toxicology. 2002 Sep 30;179(1-2):1-8
12204537
Plant Cell Physiol. 2005 Oct;46(10):1735-40
16043430
Nat Rev Immunol. 2005 Jan;5(1):43-57
15630428
Methods Enzymol. 1999;303:349-80
10349654
Food Chem Toxicol. 2005 Dec;43(12):1687-701
16139940
Arch Biochem Biophys. 1991 Apr;286(1):107-10
1654776
Annu Rev Plant Biol. 2008;59:143-66
18444899
J Plant Physiol. 2009 Apr 1;166(6):588-601
18950897
Plant Cell. 2002 Oct;14(10):2383-98
12368493
Toxicol Appl Pharmacol. 2005 Sep 1;207(2 Suppl):245-50
16002115
FEBS J. 2008 Mar;275(5):831-47
18298791
Plant Cell. 2001 Dec;13(12):2589-607
11752374
Pol Merkur Lekarski. 2008 Sep;25(147):266-8
19112846
Toxicol Sci. 2007 Jan;95(1):118-35
17079700
Trends Biochem Sci. 1991 May;16(5):173-7
1882418
Toxicol Appl Pharmacol. 2004 Oct 15;200(2):131-45
15476866
Plant Physiol Biochem. 2009 Feb;47(2):116-22
19042136
Annu Rev Entomol. 2002;47:331-59
11729078
Proc Natl Acad Sci U S A. 1991 Dec 1;88(23):10629-33
1961729
Methods Enzymol. 2007;428:209-25
17875419
Clin Toxicol (Phila). 2009 Feb;47(2):89-100
19280425
Exp Eye Res. 2009 Feb;88(2):226-34
19061888
Methods Enzymol. 1999;303:179-205
10349646
Physiol Plant. 2008 Jul;133(3):469-80
18346080
Proc Natl Acad Sci U S A. 2009 May 12;106(19):7695-701
19372371
BMC Plant Biol. 2009 Jun 05;9:69
19497134
Mol Phylogenet Evol. 2003 Dec;29(3):464-89
14615187
J Biol Chem. 1988 Nov 25;263(33):17205-8
3053703