title: Selective and sensitive detection and quantification of arylamine N-acetyltransferase 2 by a ratiometric fluorescence probe.
abstract: A novel NAT2 specific probe has been developed for sensitive detection of the activity of NAT2. Upon NAT2 catalyzed acetylation of the molecule to N-acetyl-amonafide, the ratiometric response of fluorescence with significant changes in wavelength and intensity is observed. Its significant application potential has been successfully demonstrated in cellular systems.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of Chemical Biology, East China University of Science and Technology, Shanghai 200237, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Cui
authors.forename: Ye
authors.initials: Y
authors.lastname: Zhong
authors.forename: Weiping
authors.initials: W
authors.lastname: Zhu
authors.forename: Yufang
authors.initials: Y
authors.lastname: Xu
authors.forename: Xuhong
authors.initials: X
authors.lastname: Qian
mesh_terms: D000107:Acetylation; D000225:Adenine; D001191:Arylamine N-Acetyltransferase / Q000032:analysis / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism*; D056945:Hep G2 Cells; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods; D053644:Naphthalimides / Q000737:chemistry / Q000378:metabolism*; D063065:Organophosphonates; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes; D053644:Naphthalimides; D063065:Organophosphonates; C037020:amonafide; D001191:Arylamine N-Acetyltransferase; C478900:NAT2 protein, human; D000225:Adenine