[Change of p-CREB after surgical incisional pain in rat spinal cord].
OBJECTIVE
To explore the role of phosphorylation of cAMP response element binding protein (CREB) in the incision-induced pain hypersensitivity.


METHODS
A longitudinal incision was made in one plantar hind paw of isoflurane-anesthetized rats. Spinal cords were removed at various postoperative time after behavior test. Phosphorylation of CREB was determined by immunohistochemistry and double-labeling immunofluorescence. Morphine and gabapentin were intraperitoneally injected before the behavior test and were used to determine the interaction between phosphorylation of CREB and morphine and gabapentin.


RESULTS
After the hind-paw incision, phosphorylation of CREB was enhanced in the ipsilateral lumbar spinal cord (P<0.05). The enhancement of p-CREB was mainly in the neurons in the dorsal horn of the spinal cord. All these were shown by double-labeling technique and p-CREB was mainly in the neurons. Intraperitoneal injection of morphine prevented the increased phosphorylation of CREB in the spinal cord and inhibited the mechanical allodynia induced by the incision (P>0.05). Gabapentin didn't inhibit the phosphorylation of CREB (P<0.05) but partly inhibited the mechanical allodynia.


CONCLUSIONS
Incision induces the phosphorylation of CREB in the spinal cord, and the increase of p-CREB is mainly in the neurons. Phosphorylation of CREB in the spinal cord contributes to the pain hypersensitivity induced by surgical incision.
Cui
Yulong
Y
Department of Anesthesiology, Second Xiangya Hospital, Central South University, Changsha 410011, China.
Xu
Junmei
J
Dai
Ruping
R
Li
Changqi
C
Liu
Dan
D
Yang
Longqiu
L
Wang
Juan
J
D000818:Animals; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism*; D005260:Female; D005528:Foot / Q000601:surgery; D009474:Neurons / Q000378:metabolism; D017288:Pain Threshold; D010149:Postoperative Pain / Q000378:metabolism*; D010766:Phosphorylation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000378:metabolism*
C508137:Creb1 protein, rat; D017362:Cyclic AMP Response Element-Binding Protein