[Effects of Yinqiao detoxifcation oral liquid on NK cell viability and serum concentration of IFN-gamma in SCID mouse infected by influenza virus FM1].
OBJECTIVE
To explore the dynamic effects of Yinqiao detoxifcation oral liquid (YQD) on NK cell lysis in spleen and serum concentration of IFN-gamma in SCID mouse infected by influenza A virus FM1.


METHODS
The mice were divided into six different groups randomly. Except normal control, other mice were intranasally instilled with 15 TCID of virus. Three dosage groups of YQD (5.0, 10.0, 20.0 g x kg(-1)) were respectively fed with YQD. Positive control group was administrated orally with 0.07 g x kg(-1) of ribavirin. Normal control and model group were fed with physiological saline. After 1, 3, 5 and 7 days' infection, spleens and serum were collected. Then the NK cell lysis was detected by LDH release kit and the concentration of IFN-gamma was examined by ELISA assay.


RESULTS
Contents of IFN-gamma reached to peak value on the 3th day and until the 5th day. Later, the level of IFN-gamma returned to normal level. The variation tendency of activity of NK cell in spleen was according with that of IFN-gamma. But it reached the maximum value until the 5th day. The activity of NK cell lysis in three groups of YQD was well above that i n model group. In addition, therapeutic action of both 10.0 g x kg(-1) and 20.0 g x kg(-1) of YQD treatment groups was better than that of 5.0 g x kg(-1).


CONCLUSIONS
The data showed that serum concentration of IFN-gamma and NK cell lysis were improved by YQD at different time, which was demonstrated YQD could perform well in immune system.
Ma
Rong
R
Hebei North University, Zhangjiakou 075029, China.
Xu
Yang
Y
Bi
Minggang
M
Guo
Peng
P
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage* / Q000494:pharmacology; D006801:Humans; D007251:Influenza, Human / Q000097:blood / Q000188:drug therapy* / Q000276:immunology / Q000503:physiopathology*; D029521:Alphainfluenzavirus / Q000502:physiology*; D007371:Interferon-gamma / Q000097:blood*; D007694:Killer Cells, Natural / Q000187:drug effects* / Q000502:physiology; D051379:Mice; D016513:Mice, SCID; D011897:Random Allocation; D013154:Spleen / Q000276:immunology / Q000503:physiopathology
D004365:Drugs, Chinese Herbal; C530031:yinqiao; D007371:Interferon-gamma