title: αB-crystallin is elevated in highly infiltrative apoptosis-resistant glioblastoma cells.
abstract: We have previously established two distinct glioma phenotypes by serial xenotransplantation of human glioblastoma (GBM) biopsies in nude rats. These tumors undergo a gradual transition from a highly invasive nonangiogenic to a less-invasive angiogenic phenotype. In a protein screen to identify molecular markers associated with the infiltrative phenotype, we identified α-basic-crystallin (αBc), a small heat-shock protein with cytoprotective properties. Its increased expression in the infiltrative phenotype was validated by immunohistochemistry and Western blots, confirming its identity to be tumor-derived and not from the host. Stereotactic human GBM biopsies taken from MRI-defined areas verified stronger αBc expression in the infiltrative edge compared to the tumor core. Cell migration assays and immunofluorescence staining showed αBc to be expressed by migrating cells in vitro. To determine αBc function, we altered its expression levels. αBc siRNA depletion caused a loss of migrating tumor cells from biopsy spheroids and delayed monolayer wound closure. In contrast, glioma cell migration in a Boyden chamber assay was unaffected by either αBc knockdown or overexpression, indicating that αBc is not functionally linked to the cell migration machinery. However, after siRNA αBc depletion, a significant sensitization of cells to various apoptotic inducers was observed (actinomycin, tumor necrosis factor α, and TNF-related apoptosis-inducing ligand [TRAIL]). In conclusion, αBc is overexpressed by highly migratory glioma cells where it plays a functional role in apoptosis resistance.
authors.affiliation: Department of Oncology and Medical Physics, Haukeland University Hospital, Jonas Lies vei 91, 5009 Bergen, Norway. dorota@goplen.net
authors.forename: Dorota
authors.initials: D
authors.lastname: Goplen
authors.forename: Sébastien
authors.initials: S
authors.lastname: Bougnaud
authors.forename: Uros
authors.initials: U
authors.lastname: Rajcevic
authors.forename: Stig O
authors.initials: SO
authors.lastname: Bøe
authors.forename: Kai O
authors.initials: KO
authors.lastname: Skaftnesmo
authors.forename: Juergen
authors.initials: J
authors.lastname: Voges
authors.forename: Per Ø
authors.initials: PØ
authors.lastname: Enger
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.forename: Berit B
authors.initials: BB
authors.lastname: Tysnes
authors.forename: Ole D
authors.initials: OD
authors.lastname: Laerum
authors.forename: Simone
authors.initials: S
authors.lastname: Niclou
authors.forename: Rolf
authors.initials: R
authors.lastname: Bjerkvig
mesh_terms: D000818:Animals; D017209:Apoptosis; D015153:Blotting, Western; D001921:Brain / Q000378:metabolism / Q000473:pathology; D002448:Cell Adhesion; D002465:Cell Movement; D049109:Cell Proliferation; D015180:Electrophoresis, Gel, Two-Dimensional; D005455:Fluorescent Antibody Technique; D015972:Gene Expression Regulation, Neoplastic; D005909:Glioblastoma / Q000378:metabolism* / Q000473:pathology*; D006801:Humans; D007124:Immunoenzyme Techniques; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics; D051381:Rats; D011923:Rats, Nude; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D018874:Spheroids, Cellular / Q000378:metabolism / Q000473:pathology; D053221:TNF-Related Apoptosis-Inducing Ligand / Q000378:metabolism; D046888:Tissue Array Analysis; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured; D038203:alpha-Crystallin B Chain / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D034741:RNA, Small Interfering; D053221:TNF-Related Apoptosis-Inducing Ligand; D038203:alpha-Crystallin B Chain
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