A serological and molecular study of Leishmania infantum infection in cats from the Island of Ibiza (Spain).
The aim of this study was to evaluate the prevalence of Leishmania infantum infection within a feline population by serologic and molecular methods and to identify associated risk factors. One hundred five cats living outdoors were studied. Sera were tested for IgG antibodies against L. infantum, Toxoplasma gondii, and feline immunodeficiency virus (FIV) and for the detection of feline leukemia virus (FeLV) p27 antigen by enzyme-linked immunosorbent assay (ELISA). L. infantum real-time polymerase chain reaction (PCR) was performed on DNA extracted from blood. L. infantum and T. gondii seroprevalence rates were 13.2% and 55.2%, respectively. The prevalence of L. infantum by PCR was 8.7%. The total rate of L. infantum infection derived from seroreactivity and/or positive PCR was 15.4%. Serology and PCR results were positively associated, and moderate agreement (kappa = 0.489) was found between Leishmania ELISA and PCR. No statistical association was found between positive Leishmania PCR results and gender, clinical status, or T. gondii seropositivity. Six of the 105 cats (5.7%) displayed clinical signs compatible with feline cutaneous leishmaniosis, and 4 out of these 6 cats (66.7%) were found to have Leishmania infection by means of serology and/or PCR. Leishmania seropositivity was associated with clinical signs of feline cutaneous leishmaniosis (p = 0.029). The prevalence of FeLV p27 antigen was 16.2% (17/105) and of FIV antibody was 20.9% (22/105), with coinfection found in 9.5% (10/105) of the cats. Leishmania ELISA seroreactivity and positive PCR results were statistically associated with FeLV infection and with coinfection of both retroviruses but not with a positive FIV status. The high seroprevalence and molecular rates of Leishmania infection observed indicate that cats are frequently infected with L. infantum, and the association with FeLV suggests a potential role for this retrovirus in feline Leishmania infection in endemic areas.
Sherry
Kate
K
Department of Pathology and Infectious Diseases, Royal Veterinary College, University of London, London, United Kingdom.
Miró
Guadalupe
G
Trotta
Michele
M
Miranda
Carmen
C
Montoya
Ana
A
Espinosa
Carmen
C
Ribas
Fernando
F
Furlanello
Tommaso
T
Solano-Gallego
Laia
L
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D002371:Cat Diseases / Q000453:epidemiology* / Q000469:parasitology / Q000821:virology; D002415:Cats; D060085:Coinfection; D016054:DNA, Protozoan / Q000097:blood; D016181:Feline Acquired Immunodeficiency Syndrome / Q000150:complications / Q000453:epidemiology / Q000821:virology; D005260:Female; D006801:Humans; D016089:Immunodeficiency Virus, Feline / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D007074:Immunoglobulin G / Q000097:blood; D062312:Islands / Q000453:epidemiology; D018314:Leishmania infantum / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D007898:Leishmaniasis, Visceral / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D005256:Leukemia Virus, Feline / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D016582:Leukemia, Feline / Q000150:complications / Q000453:epidemiology / Q000821:virology; D008297:Male; D012307:Risk Factors; D016036:Seroepidemiologic Studies; D013030:Spain / Q000453:epidemiology; D014122:Toxoplasma / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D014124:Toxoplasmosis, Animal / Q000150:complications / Q000453:epidemiology / Q000469:parasitology
D000913:Antibodies, Protozoan; D016054:DNA, Protozoan; D007074:Immunoglobulin G