title: Improved method for bacterial cell capture after flow cytometry cell sorting.
abstract: Fixed cells with different nucleic acid contents and scatter properties (low nucleic acid [LNA], high nucleic acid 1 [HNA1], and HNA2) were sorted by flow cytometry (FCM). For each sort, 10,000 cells were efficiently captured on poly-l-lysine-coated microplates, resulting in efficient and reproducible PCR amplification.
authors.affiliation: UPMC Université Paris 06, UMR 7621, LOMIC, Observatoire Océanologique, F-66651 Banyuls/mer, France.
authors.forename: D
authors.initials: D
authors.lastname: Guillebault
authors.forename: M
authors.initials: M
authors.lastname: Laghdass
authors.forename: P
authors.initials: P
authors.lastname: Catala
authors.forename: I
authors.initials: I
authors.lastname: Obernosterer
authors.forename: P
authors.initials: P
authors.lastname: Lebaron
mesh_terms: D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification*; D002469:Cell Separation / Q000379:methods*; D005434:Flow Cytometry / Q000379:methods*; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques / Q000379:methods; D009696:Nucleic Acids / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology
chemical_list: D009696:Nucleic Acids; D012336:RNA, Ribosomal, 16S
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