Binucleine 2, an isoform-specific inhibitor of Drosophila Aurora B kinase, provides insights into the mechanism of cytokinesis.
Aurora kinases are key regulators of cell division and important targets for cancer therapy. We report that Binucleine 2 is a highly isoform-specific inhibitor of Drosophila Aurora B kinase, and we identify a single residue within the kinase active site that confers specificity for Aurora B. Using Binucleine 2, we show that Aurora B kinase activity is not required during contractile ring ingression, providing insight into the mechanism of cytokinesis.
Smurnyy
Yegor
Y
Toms
Angela V
AV
Hickson
Gilles R
GR
Eck
Michael J
MJ
Eggert
Ulrike S
US
D000595:Amino Acid Sequence; D000818:Animals; D064107:Aurora Kinase B; D064127:Aurora Kinases; D018797:Cell Cycle Proteins / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000502:physiology*; D048749:Cytokinesis / Q000187:drug effects / Q000502:physiology*; D029721:Drosophila Proteins / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000502:physiology*; D004331:Drosophila melanogaster / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology*; D007527:Isoenzymes / Q000037:antagonists & inhibitors; D007532:Isoleucine / Q000737:chemistry; D008969:Molecular Sequence Data; D047428:Protein Kinase Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D017346:Protein Serine-Threonine Kinases / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000502:physiology*; D011720:Pyrazoles / Q000737:chemistry* / Q000494:pharmacology; D034622:RNA Interference; D012545:Schiff Bases / Q000737:chemistry* / Q000494:pharmacology
D018797:Cell Cycle Proteins; D029721:Drosophila Proteins; D007527:Isoenzymes; D047428:Protein Kinase Inhibitors; D011720:Pyrazoles; D012545:Schiff Bases; C559086:binucleine 2; D007532:Isoleucine; C576957:AurB protein, Drosophila; D064107:Aurora Kinase B; D064127:Aurora Kinases; D017346:Protein Serine-Threonine Kinases
Oncogene. 2005 Jul 28;24(32):5005-15
16049526
Mol Biol Cell. 2004 Jun;15(6):2895-906
15064354
Nature. 2008 Jun 19;453(7198):1132-6
18463638
Blood. 2002 May 1;99(9):3472-5
11964322
J Med Chem. 2009 May 14;52(9):2629-51
19320489
Cell. 1995 Apr 7;81(1):95-105
7720077
Nat Med. 2004 Mar;10(3):262-7
14981513
Curr Opin Cell Biol. 2008 Feb;20(1):77-84
18249108
J Cell Biol. 2009 Nov 30;187(5):637-53
19951914
Mol Cell. 2005 Apr 29;18(3):379-91
15866179
Curr Opin Cell Biol. 2009 Dec;21(6):796-805
19836940
Clin Cancer Res. 2007 Jun 15;13(12):3682-8
17575233
Curr Biol. 2005 Apr 26;15(8):778-86
15854913
Nat Rev Mol Cell Biol. 2003 Nov;4(11):842-54
14625535
Cell. 2009 Feb 6;136(3):473-84
19203582
J Med Chem. 2008 Sep 11;51(17):5149-71
18710211
Nat Rev Cancer. 2009 Jan;9(1):28-39
19104514
Annu Rev Biochem. 2006;75:543-66
16756502
Chem Biol. 2008 Jun;15(6):552-62
18559266
J Cell Sci. 1988 Jan;89 ( Pt 1):25-38
3417791
Nat Rev Cancer. 2005 Jan;5(1):42-50
15630414
PLoS Biol. 2004 Dec;2(12):e379
15547975
Proc Natl Acad Sci U S A. 2007 Mar 6;104(10):4106-11
17360485