title: Local hyperthermia could induce antiviral activity by endogenous interferon-dependent pathway in condyloma acuminata.
abstract: Local hyperthermia has been successfully used in the treatment of viral warts by mechanisms that have largely remained unclear. Using an organotypic culture system, we found that hyperthermia at 42 °C and 45 °C could induce a significant increase in the transcriptional expression of interferon (IFN)-α, IFN-β and IFN-γ, in a temperature-dependent manner in condyloma acuminata (CA), but not in normal skin. Accordingly, local hyperthermia could enhance the expression of 2'-5' oligoadenylate synthase and double-stranded RNA (dsRNA)-dependent protein kinase, two antiviral enzymes downstream of the IFN-dependant pathway. Hyperthermia led to an increase in IFN-α/β receptor transcripts, and an increase in the levels in phospho-Stat1 and phospho-Stat2 in CA, though it had no influence on the levels of Jak1, Tyk2, Stat1 and Stat2 transcriptional expression. Local hyperthermia was proved effective in treating human papillomavirus-infected skin. These results suggested that hyperthermia took effect partly by inducing the expression of local endogenous IFN and partly by subsequent IFN-induced antiviral activity via Jak-STATs signalling pathway in CA.
authors.affiliation: State Key Department of Dermatology, No. 1 Hospital of China Medical University, Shenyang 110001, China.
authors.forename: Li-li
authors.initials: LL
authors.lastname: Zhu
authors.forename: Xing-Hua
authors.initials: XH
authors.lastname: Gao
authors.forename: Ruiqun
authors.initials: R
authors.lastname: Qi
authors.forename: Yuxiao
authors.initials: Y
authors.lastname: Hong
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Li
authors.forename: Xiaoqin
authors.initials: X
authors.lastname: Wang
authors.forename: Uwesu Omari
authors.initials: UO
authors.lastname: McHepange
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Huachen
authors.initials: H
authors.lastname: Wei
authors.forename: Hong-Duo
authors.initials: HD
authors.lastname: Chen
mesh_terms: D015088:2',5'-Oligoadenylate Synthetase / Q000235:genetics / Q000378:metabolism; D000998:Antiviral Agents / Q000378:metabolism*; D003218:Condylomata Acuminata / Q000473:pathology / Q000628:therapy* / Q000821:virology; D004268:DNA-Binding Proteins / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D006358:Hot Temperature; D052140:Human papillomavirus 11 / Q000502:physiology; D052163:Human papillomavirus 6 / Q000502:physiology; D006801:Humans; D006979:Hyperthermia, Induced; D016898:Interferon-alpha / Q000235:genetics / Q000378:metabolism; D016899:Interferon-beta / Q000235:genetics / Q000378:metabolism; D007371:Interferon-gamma / Q000235:genetics / Q000378:metabolism; D007372:Interferons / Q000235:genetics / Q000378:metabolism*; D008297:Male; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D050791:STAT Transcription Factors / Q000378:metabolism; D015398:Signal Transduction; D012867:Skin / Q000378:metabolism / Q000473:pathology; D014779:Virus Replication; D019892:eIF-2 Kinase / Q000235:genetics / Q000378:metabolism
chemical_list: D000998:Antiviral Agents; D004268:DNA-Binding Proteins; D016898:Interferon-alpha; D050791:STAT Transcription Factors; D016899:Interferon-beta; D007371:Interferon-gamma; D007372:Interferons; D011505:Protein-Tyrosine Kinases; D019892:eIF-2 Kinase; D015088:2',5'-Oligoadenylate Synthetase