title: Antibody response to Haemophilus influenzae outer membrane protein D, P6, and OMP26 after nasopharyngeal colonization and acute otitis media in children.
abstract: Development of natural antibodies to 3 nontypeable Haemophilus influenzae (NTHi) outer membrane proteins (D, P6 and OMP26) was prospectively studied in 130 children 6-30 months of age during NP colonization and acute otitis media (AOM). IgG antibody to protein D, P6 and OMP26 increased with age (p<0.001). Serum IgG responses to NP colonization were different for the 3 proteins: protein D responses occurred at a later age than P6, and OMP26 responses were minimal. For all 3 proteins serum antibody levels in the convalescent phase of AOM infection were not as high as after NP colonization. Antibodies to protein D and P6 but not OMP26 were bactericidal.
authors.affiliation: Rochester General Hospital Research Institue, 1425 Portland Avenue, Rochester, NY 14621, USA. michael.pichichero@rochestergeneral.org
authors.forename: Michael E
authors.initials: ME
authors.lastname: Pichichero
authors.forename: Ravinder
authors.initials: R
authors.lastname: Kaur
authors.forename: Janet R
authors.initials: JR
authors.lastname: Casey
authors.forename: Albert
authors.initials: A
authors.lastname: Sabirov
authors.forename: M Nadeem
authors.initials: MN
authors.lastname: Khan
authors.forename: Anthony
authors.initials: A
authors.lastname: Almudevar
mesh_terms: D000907:Antibodies, Bacterial / Q000097:blood*; D000917:Antibody Formation; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D001426:Bacterial Proteins / Q000276:immunology; D002352:Carrier Proteins / Q000276:immunology; D002675:Child, Preschool; D006192:Haemophilus Infections / Q000276:immunology*; D018073:Haemophilus Vaccines / Q000276:immunology*; D006193:Haemophilus influenzae / Q000145:classification / Q000276:immunology; D006801:Humans; D007072:Immunoglobulin D / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood; D007223:Infant; D008074:Lipoproteins / Q000276:immunology; D058885:Multilocus Sequence Typing; D009305:Nasopharynx / Q000382:microbiology*; D010033:Otitis Media / Q000276:immunology / Q000382:microbiology*; D011446:Prospective Studies
chemical_list: D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D002352:Carrier Proteins; D018073:Haemophilus Vaccines; D007072:Immunoglobulin D; D007074:Immunoglobulin G; D008074:Lipoproteins; C118414:OMP26 protein, Haemophilus influenzae; C069990:P6 outer membrane protein, Haemophilus; C067989:glpQ protein, Haemophilus influenzae
references.citation: J Infect Dis. 1989 Dec;160(6):999-1004
references.pmid: 2584766
references.citation: J Pediatr. 1993 Feb;122(2):212-8
references.pmid: 8429433
references.citation: J Infect Dis. 1995 Jul;172(1):132-5
references.pmid: 7797903
references.citation: Infect Immun. 1980 Dec;30(3):709-17
references.pmid: 6971807
references.citation: J Infect Dis. 1975 Dec;132(6):685-8
references.pmid: 1104722
references.citation: J Clin Invest. 1986 Oct;78(4):1020-7
references.pmid: 2428838
references.citation: Infect Immun. 1989 Nov;57(11):3555-9
references.pmid: 2807536
references.citation: J Pediatr. 1980 Sep;97(3):364-9
references.pmid: 6967963
references.citation: Lancet. 2006 Mar 4;367(9512):740-8
references.pmid: 16517274
references.citation: Infect Immun. 1998 May;66(5):2272-8
references.pmid: 9573117
references.citation: Pediatr Infect Dis J. 2007 Oct;26(10 Suppl):S12-6
references.pmid: 18049375
references.citation: Acta Otolaryngol. 1993 Jul;113(4):524-9
references.pmid: 8379309
references.citation: J Clin Microbiol. 1976 Sep;4(3):306-8
references.pmid: 9424
references.citation: J Infect Dis. 2007 Jan 1;195(1):81-9
references.pmid: 17152011
references.citation: J Biotechnol. 1999 Aug 20;73(2-3):103-8
references.pmid: 10486920
references.citation: Otolaryngol Head Neck Surg. 1991 Sep;105(3):406-10
references.pmid: 1945426
references.citation: Clin Infect Dis. 2008 Mar 1;46(5):726-31
references.pmid: 18230042
references.citation: Infect Immun. 2000 Apr;68(4):2119-28
references.pmid: 10722609
references.citation: Infect Immun. 1996 Nov;64(11):4586-92
references.pmid: 8890211
references.citation: Acta Otolaryngol. 1999;119(6):703-7
references.pmid: 10587005
references.citation: J Infect Dis. 1994 Oct;170(4):862-6
references.pmid: 7930728
references.citation: Pediatr Infect Dis J. 1996 Feb;15(2):128-33
references.pmid: 8822285
references.citation: Pediatr Infect Dis J. 2010 Apr;29(4):304-9
references.pmid: 19935445
references.citation: Infect Immun. 2000 Mar;68(3):1569-73
references.pmid: 10678976
references.citation: Pediatr Res. 2009 Nov;66(5):565-70
references.pmid: 19581824
references.citation: Infect Immun. 1996 Dec;64(12):5187-92
references.pmid: 8945564
references.citation: Infect Immun. 1982 May;36(2):535-40
references.pmid: 6979511
references.citation: J Infect Dis. 1986 Jul;154(1):100-9
references.pmid: 3486923
references.citation: Infect Immun. 2003 Aug;71(8):4691-9
references.pmid: 12874350
references.citation: Acta Otolaryngol. 1998 Nov;118(6):826-32
references.pmid: 9870628
references.citation: J Infect Dis. 1983 May;147(5):838-46
references.pmid: 6601684
references.citation: Infect Immun. 1998 Jun;66(6):2453-9
references.pmid: 9596702
references.citation: Vaccine. 2000 Dec 8;19 Suppl 1:S41-50
references.pmid: 11163462
references.citation: Expert Rev Vaccines. 2006 Aug;5(4):517-34
references.pmid: 16989632
references.citation: Vaccine. 2002 Oct 4;20(29-30):3590-7
references.pmid: 12297406
references.citation: Otolaryngol Head Neck Surg. 1997 Mar;116(3):363-71
references.pmid: 9121792
references.citation: Rev Inst Med Trop Sao Paulo. 1998 Jan-Feb;40(1):7-9
references.pmid: 9713130
references.citation: Lancet. 2000 Dec 16;356(9247):2065-6
references.pmid: 11145494
references.citation: Pediatr Infect Dis J. 2004 Sep;23(9):824-8
references.pmid: 15361720
references.citation: Pediatr Infect Dis J. 2001 May;20(5):482-7
references.pmid: 11368104