[Identification of Mycoplasma in patients with suspected prostate cancer].
OBJECTIVE
Tests for Mycoplasma hominis, M. genitalium, Ureaplasma urealyticum in males with suspected prostate cancer.


METHODS
Identification of mycoplasms was performed in prostate tissue samples using universal PCR as well as in serum samples of patients with suspected prostate cancer using ELISA for detection of IgG to M. hominis. Two hundred and fifty samples from each lobe of prostate were obtained from 125 patients with suspected prostate cancer by transrectal polyfocal biopsy. Blood samples were drawn from the same patients for ELISA.


RESULTS
Out of 125 patients with suspected prostate cancer, 20.5% were positive for Mycoplasma by PCR. Between studied species, only M. hominis was found in big proportion of analyzed samples. Out of 118 serum samples, 30.5% were positive for IgG to M. hominis in ELISA.


CONCLUSIONS
Fact of presence of Mycoplasma species in tissue of prostate was established in 20.5% pf patients with suspected prostate cancer. Obtained results show that M. hominis is frequently infects prostate tissue and that this infection was more common in patients with high grade prostatic interstitial neoplasia and prostate cancer than in patients with benign changes of prostate tissue or in persons without prostate disease. This allows to suggest that infection with M. hominis could play an important role in development of cancer.
Barykova
Iu A
IuA
Shmarov
M M
MM
Logunov
D Iu
DIu
Verkhovskaia
L V
LV
Aliaev
Iu G
IuG
Fiev
D N
DN
Vinarov
A Z
AZ
Vinarova
N A
NA
Rakovskaia
I V
IV
Naroditskiî
B S
BS
Gudkov
A V
AV
Gintsburg
A L
AL
D000368:Aged; D000369:Aged, 80 and over; D000907:Antibodies, Bacterial / Q000097:blood; D004269:DNA, Bacterial / Q000235:genetics; D006801:Humans; D008297:Male; D008875:Middle Aged; D009175:Mycoplasma Infections / Q000150:complications / Q000175:diagnosis*; D045704:Mycoplasma genitalium / Q000302:isolation & purification; D019535:Mycoplasma hominis / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D011470:Prostatic Hyperplasia / Q000382:microbiology*; D019048:Prostatic Intraepithelial Neoplasia / Q000382:microbiology*; D011471:Prostatic Neoplasms / Q000382:microbiology*; D014509:Ureaplasma / Q000302:isolation & purification; D016869:Ureaplasma Infections / Q000150:complications / Q000175:diagnosis
D000907:Antibodies, Bacterial; D004269:DNA, Bacterial