[Investigation of metallo-beta-lactamase-producing Acinetobacter sp and Pseudomonas aeruginosa at an emergency hospital in Porto Alegre, State of Rio Grande do Sul, Brazil].
BACKGROUND
The appearance of metallo-beta-lactamase (MBL)-producing Pseudomonas aeruginosa and Acinetobacter sp. is a challenge for hospitals.


METHODS
The production of MBL in clinical isolates of Pseudomonas aeruginosa and Acinetobacter sp. From an emergency hospital in Porto Alegre was investigated using the disk approximation test and MBL E-test. The bla genes were determined using PCR.


RESULTS
Two strains of Pseudomonas aeruginosa and eight of Acinetobacter sp were shown to be MBL phenotypes. Amplification of the blaSPM-1 gene confirmed the presence of the enzyme in P. aeruginosa.


CONCLUSIONS
Caution is needed in evaluating phenotype tests used for routine detection of metallo-beta-lactamases.
Laranjeira
Vani Dos Santos
Vdos S
Instituto de Ciências da Saúde, Universidade Feevale, Novo Hamburgo, RS.
Marchetti
Desiree Padilha
DP
Steyer
Juçara Rodrigues
JR
Corção
Gertrudes
G
Picoli
Simone Ulrich
SU
D000150:Acinetobacter / Q000187:drug effects / Q000201:enzymology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001938:Brazil; D005798:Genes, Bacterial / Q000235:genetics*; D006801:Humans; D008826:Microbial Sensitivity Tests; D010641:Phenotype; D016133:Polymerase Chain Reaction; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000201:enzymology*; D001618:beta-Lactamases / Q000096:biosynthesis* / Q000235:genetics
D000900:Anti-Bacterial Agents; D001618:beta-Lactamases