title: Photophysical evaluation of mTHPC-loaded HSA nanoparticles as novel PDT delivery systems.
abstract: Controlled drug release is one of the main goals of recent developments in drug carrier systems. In this work human serum albumin (HSA) nanoparticles as carriers for 5-, 10-, 15-, 20-Tetrakis (3-hydroxyphenyl)-chlorin (mTHPC) were investigated. The photophysical properties of mTHPC-HSA nanoparticles in dependence of loading ratio and level of HSA cross-linking were determined. Further the drug release after uptake by Jurkat cells and in vitro singlet oxygen kinetics were examined. The loading ratio of the mTHPC-HSA nanoparticles turned out to be of major importance for the PDT relevant electronic parameters in solution. Therefore, only HSA nanoparticles with low mTHPC-loading ratio generate singlet oxygen in D(2)O. However, after cellular uptake all mTHPC-HSA samples generate singlet oxygen in Jurkat cells, but the decomposition rate depends on the level of HSA cross-linking.
authors.affiliation: Institute of Physics, Humboldt Universität zu Berlin, Newtonstraße 15, 12489 Berlin, Germany.
authors.forename: Kuan
authors.initials: K
authors.lastname: Chen
authors.forename: Matthias
authors.initials: M
authors.lastname: Wacker
authors.forename: Steffen
authors.initials: S
authors.lastname: Hackbarth
authors.forename: Carmen
authors.initials: C
authors.lastname: Ludwig
authors.forename: Klaus
authors.initials: K
authors.lastname: Langer
authors.forename: Beate
authors.initials: B
authors.lastname: Röder
mesh_terms: D004337:Drug Carriers / Q000737:chemistry; D006801:Humans; D019169:Jurkat Cells; D008652:Mesoporphyrins / Q000008:administration & dosage*; D053758:Nanoparticles / Q000737:chemistry*; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000008:administration & dosage*; D012709:Serum Albumin / Q000737:chemistry*; D026082:Singlet Oxygen / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet
chemical_list: D004337:Drug Carriers; D008652:Mesoporphyrins; D017319:Photosensitizing Agents; D012709:Serum Albumin; D026082:Singlet Oxygen; C072269:temoporfin