title: Photoreceptor cells constitutively express functional TLR4.
abstract: Toll-like receptor 4 (TLR4) is expressed on a number of cells including neurons in the brain. However, it has yet to be determined if TLR4 is expressed on photoreceptor cells in the retina. In this report, we examined primary photoreceptor cells and an established photoreceptor cell line (661W). We found that functional TLR4 is constitutively expressed on photoreceptor cells, and can be activated by LPS. We conclude that TLR4 on photoreceptor cells could directly contribute to retinal inflammatory diseases and photoreceptor cell survival.
authors.affiliation: Department of Pathology, Case Western Reserve University, Cleveland 44106, USA.
authors.forename: Zhidan
authors.initials: Z
authors.lastname: Tu
authors.forename: Jose-Andres C
authors.initials: JA
authors.lastname: Portillo
authors.forename: Scott
authors.initials: S
authors.lastname: Howell
authors.forename: Hong
authors.initials: H
authors.lastname: Bu
authors.forename: Carlos S
authors.initials: CS
authors.lastname: Subauste
authors.forename: Muayyad R
authors.initials: MR
authors.lastname: Al-Ubaidi
authors.forename: Eric
authors.initials: E
authors.lastname: Pearlman
authors.forename: Feng
authors.initials: F
authors.lastname: Lin
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology; D002469:Cell Separation; D054360:Chemokine CXCL1 / Q000096:biosynthesis / Q000276:immunology; D005434:Flow Cytometry; D007249:Inflammation / Q000276:immunology / Q000378:metabolism; D015850:Interleukin-6 / Q000096:biosynthesis / Q000276:immunology; D007834:Lasers; D008070:Lipopolysaccharides / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D042282:Microdissection; D020419:Photoreceptor Cells, Vertebrate / Q000276:immunology* / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051197:Toll-Like Receptor 4 / Q000096:biosynthesis* / Q000276:immunology*
chemical_list: D054360:Chemokine CXCL1; D015850:Interleukin-6; D008070:Lipopolysaccharides; C493487:Tlr4 protein, mouse; D051197:Toll-Like Receptor 4
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