title: Effects of focused ultrasound and microbubbles on the vascular permeability of nanoparticles delivered into mouse tumors.
abstract: Ultrasound sonication with microbubbles (MBs) was evaluated for enhancement of the release of nanoparticles from vasculature to tumor tissues. In this study, tumor-bearing Balb/c mice were insonicated with focused ultrasound (FUS) in the tumors after the injection of MBs (SonoVue) and then lipid-coated quantum dot (LQD) nanoparticles (130 +/- 25 nm) were injected through the tail vein. We studied the effects of the injected MB dose (0-300 microL/kg), sonication duration (0-300 s) and treatment-procedure sequence on the accumulation of nanoparticles in the tumors 24 h after the treatment and the time response of the accumulation (0.5-24 h). After the treatment, the mice were sacrificed and perfused and then the tumor tissues were harvested for quantifying the amount of nanoparticles using graphite furnace atomic absorption spectrometry (GF-AAS). The results showed that pulsed-FUS sonication with MBs can effectively enhance the vascular permeability for LQD nanoparticle delivery into the sonicated tumors. It indicates that this technique is promising for a better nanodrug delivery for tumor chemotherapy.
authors.affiliation: Institute of Biomedical Engineering, National Taiwan University, Taipei, Taiwan.
authors.forename: Chung-Yin
authors.initials: CY
authors.lastname: Lin
authors.forename: Yen-Lin
authors.initials: YL
authors.lastname: Huang
authors.forename: Jia-Rong
authors.initials: JR
authors.lastname: Li
authors.forename: Fu-Hsiung
authors.initials: FH
authors.lastname: Chang
authors.forename: Win-Li
authors.initials: WL
authors.lastname: Lin
mesh_terms: D000230:Adenocarcinoma / Q000188:drug therapy*; D000818:Animals; D019187:Cadmium Compounds / Q000493:pharmacokinetics; D002199:Capillary Permeability; D015179:Colorectal Neoplasms / Q000188:drug therapy*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D045423:Microbubbles / Q000627:therapeutic use*; D045663:Quantum Dots; D018036:Selenium Compounds / Q000493:pharmacokinetics; D013054:Spectrophotometry, Atomic; D013997:Time Factors; D014407:Tumor Cells, Cultured
chemical_list: D019187:Cadmium Compounds; D018036:Selenium Compounds; C058667:cadmium selenide