title: Movement within and movement beyond: synaptotagmin-mediated vesicle fusion during chemotaxis.
abstract: Leukocyte chemotaxis plays an essential role in generating and delivering immune responses and is a critical component of inflammation. In order to identify novel genes and pathways important for regulating chemotaxis, we performed an RNAi-based screen and identified several genes involved with vesicle movement and fusion as mediators of chemotaxis. Our recently published data show that during chemotaxis vesicle trafficking proteins are required for lysosome fusion, uropod release and efficient directed cell migration.
authors.affiliation: Division of Rheumatology, Allergy and Immunology, Massachusetts General Hospital, Boston, MA, USA.
authors.forename: Richard A
authors.initials: RA
authors.lastname: Colvin
authors.forename: Andrew D
authors.initials: AD
authors.lastname: Luster
mesh_terms: D001692:Biological Transport; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology*; D002634:Chemotaxis, Leukocyte / Q000502:physiology*; D015723:Gene Library; D006801:Humans; D052118:Lysosomal-Associated Membrane Protein 1 / Q000378:metabolism; D008561:Membrane Fusion; D034622:RNA Interference; D054387:Receptors, CXCR / Q000378:metabolism; D050857:Synaptotagmins / Q000235:genetics* / Q000378:metabolism*; D020691:rab GTP-Binding Proteins / Q000378:metabolism
chemical_list: D052118:Lysosomal-Associated Membrane Protein 1; D054387:Receptors, CXCR; D050857:Synaptotagmins; D020691:rab GTP-Binding Proteins
references.citation: Exp Cell Res. 1970 Mar;59(3):393-8
references.pmid: 4907703
references.citation: Exp Cell Res. 1970 Jun;60(3):437-44
references.pmid: 5463639
references.citation: Exp Cell Res. 1970 Oct;62(2):389-98
references.pmid: 5531377
references.citation: J Biol Chem. 2004 May 7;279(19):20471-9
references.pmid: 14993220
references.citation: J Cell Sci. 2007 Jul 15;120(Pt 14):2318-27
references.pmid: 17606987
references.citation: Science. 1990 Mar 9;247(4947):1229-33
references.pmid: 2315695
references.citation: Nat Immunol. 2010 Jun;11(6):495-502
references.pmid: 20473299
references.citation: J Immunol. 1974 Jun;112(6):2047-54
references.pmid: 4596695
references.citation: J Immunol. 1974 Jun;112(6):2055-62
references.pmid: 4825785
references.citation: Biochem Biophys Res Commun. 2002 May 10;293(3):899-906
references.pmid: 12051743
references.citation: Dev Cell. 2005 Nov;9(5):663-73
references.pmid: 16256741
references.citation: Am J Pathol. 1976 Nov;85(2):385-94
references.pmid: 793409
references.citation: J Clin Invest. 1979 Jul;64(1):8-16
references.pmid: 447862
references.citation: Annu Rev Pharmacol Toxicol. 2008;48:171-97
references.pmid: 17883327
references.citation: J Cell Biol. 1994 Jun;125(6):1265-74
references.pmid: 7515888
references.citation: Biol Cell. 2007 May;99(5):261-71
references.pmid: 17288539
references.citation: J Cell Sci. 2007 Jan 15;120(Pt 2):219-28
references.pmid: 17179206
references.citation: Cold Spring Harb Symp Quant Biol. 1962;27:351-65
references.pmid: 13932922
references.citation: J Exp Med. 1999 May 17;189(10):1649-58
references.pmid: 10330444