title: Neutralizing antibodies to human and simian adenoviruses in humans and New-World monkeys.
abstract: Vaccines based on adenovirus (Ad) vectors are currently in development against several pathogens. However, neutralizing antibodies (NAb) to human adenovirus type 5 (AdHu5), the best-studied vector, are highly prevalent in humans worldwide. Less-prevalent adenoviruses, including human and simian serotypes, provide alternative vaccine platforms. In this study, sera from 200 Brazilian human subjects and New-World monkeys were tested for NAb titers to human serotypes AdHu5 and AdHu26 and chimpanzee-origin Ad viruses of serotype 6 (AdC6) and serotype 68 (AdC68). Seroprevalence rates of NAb in humans were 69.5% for AdHu5, 44% for AdHu26, 21% for AdC6 and 23.5% for AdC68. In addition, NAb titers to human Ad were consistently higher than those found to simian serotypes. Surprisingly, sera from some New-World monkey species were able to neutralize AdC6 and/or AdC68. A possible explanation for these findings and the implications for the development of Ad-vector vaccines are discussed in detail.
authors.affiliation: Departamento de Microbiologia, Imunologia e Parasitologia, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, 88040-900, Florianópolis, SC, Brazil.
authors.forename: Jonatan
authors.initials: J
authors.lastname: Ersching
authors.forename: Malva I M
authors.initials: MI
authors.lastname: Hernandez
authors.forename: Fabrizzio S
authors.initials: FS
authors.lastname: Cezarotto
authors.forename: Jovino D S
authors.initials: JD
authors.lastname: Ferreira
authors.forename: Amely B
authors.initials: AB
authors.lastname: Martins
authors.forename: William M
authors.initials: WM
authors.lastname: Switzer
authors.forename: Zhiquan
authors.initials: Z
authors.lastname: Xiang
authors.forename: Hildegund C J
authors.initials: HC
authors.lastname: Ertl
authors.forename: Carlos R
authors.initials: CR
authors.lastname: Zanetti
authors.forename: Aguinaldo R
authors.initials: AR
authors.lastname: Pinto
mesh_terms: D000257:Adenoviridae Infections / Q000276:immunology* / Q000662:veterinary*; D000260:Adenoviruses, Human / Q000276:immunology*; D000261:Adenoviruses, Simian / Q000276:immunology*; D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood*; D000914:Antibodies, Viral / Q000097:blood*; D001938:Brazil; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D051078:Platyrrhini; D018419:Primate Diseases / Q000276:immunology* / Q000821:virology; D016036:Seroepidemiologic Studies; D055815:Young Adult
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral