Radiation desiccation response motif-like sequences are involved in transcriptional activation of the Deinococcal ssb gene by ionizing radiation but not by desiccation.
Single-stranded-DNA binding protein (SSB) levels during poststress recovery of Deinococcus radiodurans were significantly enhanced by (60)Co gamma rays or mitomycin C treatment but not by exposure to UV rays, hydrogen peroxide (H₂O₂), or desiccation. Addition of rifampin prior to postirradiation recovery blocked such induction. In silico analysis of the ssb promoter region revealed a 17-bp palindromic radiation/desiccation response motif (RDRM1) at bp -114 to -98 and a somewhat similar sequence (RDRM2) at bp -213 to -197, upstream of the ssb open reading frame. Involvement of these cis elements in radiation-responsive ssb gene expression was assessed by constructing transcriptional fusions of edited versions of the ssb promoter region with a nonspecific acid phosphatase encoding reporter gene, phoN. Recombinant D. radiodurans strains carrying such constructs clearly revealed (i) transcriptional induction of the ssb promoter upon irradiation and mitomycin C treatment but not upon UV or H₂O₂ treatment and (ii) involvement of both RDRM-like sequences in such activation of SSB expression, in an additive manner.
Ujaoney
Aman Kumar
AK
Molecular Biology Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400 085, India.
Potnis
Akhilesh A
AA
Kane
Pratiksha
P
Mukhopadhyaya
Rita
R
Apte
Shree Kumar
SK
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D004249:DNA Damage; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D034301:Deinococcus / Q000235:genetics* / Q000378:metabolism* / Q000528:radiation effects; D003890:Desiccation; D005720:Gamma Rays; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects / Q000528:radiation effects*; D006861:Hydrogen Peroxide; D008969:Molecular Sequence Data; D014158:Transcription, Genetic; D014466:Ultraviolet Rays
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D006861:Hydrogen Peroxide
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