title: Protective effects of transduced PEP-1-Frataxin protein on oxidative stress-induced neuronal cell death.
abstract: Reactive oxygen species (ROS) actively contribute to the development of a number of human diseases including ischemia. In response to oxidative stress, frataxin has a significant ability to improve cell survival though its biological function is unclear in relation to ischemia. To explore frataxin's role in protecting against ischemic cell death, we constructed PEP-1-Frataxin cell-permeable fusion protein. In a dose- and time-dependent manner PEP-1-Frataxin rapidly transduced into astrocyte cells and protected them against oxidative stress-induced cell death. Further, using an animal model, immunohistochemical analysis revealed that PEP-1-Frataxin prevented neuronal cell death in the CA1 region of the hippocampus induced by transient forebrain ischemia. These results demonstrate that transduced PEP-1-Frataxin protects against cell death in vitro and in vivo, suggesting that transduction of PEP-1-Frataxin could be useful as a therapeutic agent for various human diseases related to oxidative stress.
authors.affiliation: Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University, Chunchon 200-702, Republic of Korea.
authors.forename: Mi Jin
authors.initials: MJ
authors.lastname: Kim
authors.forename: Dae Won
authors.initials: DW
authors.lastname: Kim
authors.forename: Ki-Yeon
authors.initials: KY
authors.lastname: Yoo
authors.forename: Eun Jeong
authors.initials: EJ
authors.lastname: Sohn
authors.forename: Hoon Jae
authors.initials: HJ
authors.lastname: Jeong
authors.forename: Hye Won
authors.initials: HW
authors.lastname: Kang
authors.forename: Min Jea
authors.initials: MJ
authors.lastname: Shin
authors.forename: Eun Hee
authors.initials: EH
authors.lastname: Ahn
authors.forename: Jae Jin
authors.initials: JJ
authors.lastname: An
authors.forename: Soon Won
authors.initials: SW
authors.lastname: Kwon
authors.forename: Young Nam
authors.initials: YN
authors.lastname: Kim
authors.forename: Moo Ho
authors.initials: MH
authors.lastname: Won
authors.forename: Sung-Woo
authors.initials: SW
authors.lastname: Cho
authors.forename: Jinseu
authors.initials: J
authors.lastname: Park
authors.forename: Won Sik
authors.initials: WS
authors.lastname: Eum
authors.forename: Soo Young
authors.initials: SY
authors.lastname: Choi
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000187:drug effects; D001812:Blood-Brain Barrier / Q000378:metabolism; D056547:CA1 Region, Hippocampal / Q000473:pathology; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D004396:Coloring Agents; D003543:Cysteamine / Q000031:analogs & derivatives* / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005822:Genetic Vectors; D005849:Gerbillinae; D006801:Humans; D033862:Iron-Binding Proteins / Q000096:biosynthesis / Q000494:pharmacology*; D002546:Ischemic Attack, Transient / Q000188:drug therapy / Q000473:pathology; D008856:Microscopy, Fluorescence; D009474:Neurons / Q000187:drug effects*; D018696:Neuroprotective Agents; D018384:Oxidative Stress / Q000187:drug effects*; D010455:Peptides / Q000494:pharmacology*; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000494:pharmacology; D013778:Tetrazolium Salts; D013844:Thiazoles; D014161:Transduction, Genetic; D000096923:Frataxin
chemical_list: D004396:Coloring Agents; D003543:Cysteamine; D033862:Iron-Binding Proteins; D018696:Neuroprotective Agents; D010455:Peptides; D011993:Recombinant Fusion Proteins; D013778:Tetrazolium Salts; D013844:Thiazoles; D000096923:Frataxin; C503924:Pep-1 peptide; C022616:thiazolyl blue