title: Assessment of isorhamnetin 3-O-neohesperidoside from Acacia salicina: protective effects toward oxidation damage and genotoxicity induced by aflatoxin B1 and nifuroxazide.
abstract: Antioxidant activity of isorhamnetin 3-O-neohesperidoside, isolated from the leaves of Acacia salicina, was determined by the ability of this compound to inhibit xanthine oxidase activity and to scavenge the free radical 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS(.-)) diammonium salt. Antigenotoxic activity was assessed using the SOS chromotest assay. This compound has the ability to scavenge the ABTS(.+) radical by a hydrogen donating mechanism. We also envisaged the study of the antioxidant effect of this compound by the enzymatic xanthine/xanthine oxidase (X/XOD) assay. Results indicated that isorhamnetin 3-O-neohesperidoside was a potent inhibitor of xanthine oxidase and superoxide anion scavengers. Moreover, this compound induced an inhibitory activity against nifuroxazide and aflatoxine B1 (AFB1) induced genotoxicity. Taken together, these observations provide evidence that isorhamnetin 3-O-neohesperidoside isolated from the leaves of A. salicina is able to protect cells against the consequences of oxidative stress.
authors.affiliation: Unité de Pharmacognosie/Biologie Moléculaire 99/UR/07-03. Faculté de Pharmacie de Monastir, Rue Avicenne 5000 Monastir, Tunisia.
authors.forename: Ines
authors.initials: I
authors.lastname: Bouhlel
authors.forename: Ilef
authors.initials: I
authors.lastname: Limem
authors.forename: Ines
authors.initials: I
authors.lastname: Skandrani
authors.forename: Aicha
authors.initials: A
authors.lastname: Nefatti
authors.forename: Kamel
authors.initials: K
authors.lastname: Ghedira
authors.forename: Marie-Genevieve
authors.initials: MG
authors.lastname: Dijoux-Franca
authors.forename: Chekir-Ghedira
authors.initials: CG
authors.lastname: Leila
mesh_terms: D000045:Acacia / Q000737:chemistry*; D016604:Aflatoxin B1 / Q000633:toxicity*; D000818:Animals; D016587:Antimutagenic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D045643:DNA Repair Enzymes / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D044948:Flavonols / Q000302:isolation & purification / Q000494:pharmacology; D005609:Free Radicals / Q000737:chemistry; D062385:Hydroxybenzoates / Q000633:toxicity*; D008862:Microsomes, Liver / Q000187:drug effects; D009152:Mutagenicity Tests; D009581:Nitrofurans / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D051381:Rats; D014969:Xanthine Oxidase / Q000037:antagonists & inhibitors
chemical_list: D016587:Antimutagenic Agents; D000975:Antioxidants; D044948:Flavonols; D005609:Free Radicals; D062385:Hydroxybenzoates; D009581:Nitrofurans; D010936:Plant Extracts; C469309:isorhamnetin-3-O-neohesperidoside; D016604:Aflatoxin B1; D014969:Xanthine Oxidase; D045643:DNA Repair Enzymes; C013150:nifuroxazide