A duplex PCR method to identify mosaic penA gene and predict reduced susceptibility to oral cephalosporins in Neisseria gonorrhoeae.
Neisseria gonorrhoeae with mosaic penicillin-binding protein 2 (PBP2) is associated with reduced susceptibility to third-generation oral cephalosporins. A simple and rapid PCR method using three primers was designed to identify mosaic PBP2, which could help predict reduced susceptibility to expanded-spectrum cephalosporins in N. gonorrhoeae.
Huang
Chung-Ter
CT
Research and Diagnostic Center, Centers for Disease Control, Taipei, Taiwan.
Niu
Jennifer
J
Liao
Mei-Hui
MH
Li
Shu-Ying
SY
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002511:Cephalosporins / Q000494:pharmacology*; D017931:DNA Primers / Q000235:genetics; D005838:Genotype; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods; D009344:Neisseria gonorrhoeae / Q000187:drug effects* / Q000235:genetics*; D046915:Penicillin-Binding Proteins / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D013997:Time Factors
D000900:Anti-Bacterial Agents; D002511:Cephalosporins; D017931:DNA Primers; D046915:Penicillin-Binding Proteins