A simple chromatographic route for the isolation of meso diaminopimelic acid.
Meso diaminopimelic acid is an important noncoded amino acid found in Gram-negative bacterial peptidoglycan. In spite of its importance, this stereoisomer is not available commercially. A simple, economical procedure was developed for the isolation of pure meso diaminopimelic acid via an high-performance liquid chromatography separation. In our new approach, the underivatized three isomers of diaminopimelic acid were separated on a crown ether-based chiral stationary phase. For the structure identification, (1)H NMR spectroscopy was applied.
Tóth
Gábor K
GK
Department of Medical Chemistry, University of Szeged, Szeged, Hungary.
Hetényi
Anasztázia
A
Ilisz
István
I
Péter
Antal
A
D056148:Chromatography, Reverse-Phase / Q000379:methods*; D043844:Crown Ethers / Q000737:chemistry*; D003960:Diaminopimelic Acid / Q000138:chemical synthesis* / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy; D013237:Stereoisomerism
D043844:Crown Ethers; D003960:Diaminopimelic Acid