Crossbridge and non-crossbridge contributions to force in shortening and lengthening muscle.
Analysis of tension responses to ramp length changes in muscle can provide important information about the crossbridge cycle. During a ramp length change, the force response of an active muscle shows an early change in slope (the P₁ transition) followed by a later, gradual change in slope (the P₂ transition). Modeling shows that the first transition reflects the tension change associated with the crossbridge power stroke in shortening and with its reversal in lengthening; the reduction in slope at the second transition occurs when most of the crossbridges (myosin heads) that were attached at the start of the ramp become detached; the steady tension during shortening is borne mainly by post-stroke heads whereas tension during lengthening is borne mostly by pre-stroke heads. After the P₂ transition, the tension reaches a steady level in the model whereas in the experiments the tension continues to increase during lengthening or to decrease during shortening; this tension change is seen at a wide range of sarcomere lengths and even when active force is reduced by a myosin inhibitor. It appears that some non-crossbridge components in muscle fibers stiffen upon activation and contribute to the continued tension rise during lengthening; release of such tension leads to tension decline during shortening. Thus, non-crossbridge visco-elasticity in sarcomeres may also contribute to energy storage and release during in situ muscle function.
Ranatunga
K W
KW
Muscle Contraction Group, Department of Physiology and Pharmacology, School of Medical Sciences, University of Bristol, Bristol, UK. k.w.ranatunga@bris.ac.uk
Roots
H
H
Pinniger
G J
GJ
Offer
G W
GW
D000244:Adenosine Diphosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D008297:Male; D008954:Models, Biological; D009119:Muscle Contraction / Q000502:physiology*; D009126:Muscle Relaxation / Q000502:physiology*; D018482:Muscle, Skeletal / Q000502:physiology*; D009218:Myosins / Q000502:physiology; D051381:Rats; D012518:Sarcomeres / Q000502:physiology; D013314:Stress, Mechanical
D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D009218:Myosins