title: [Changes of Foxp3 and IL-10 and TGF-beta in aging of mice].
abstract: OBJECTIVE
  To compare the expression levels of Foxp3 mRNA in the spleen PBMC (peripheral blood mononuclear cell) in the progress of aging mice and analyse the relevance with serum IL-10 and TGF-beta.
  
  
  METHODS
  Healthy male BALB/c mice were divided into 3 groups at random (8 mice each group): 2m group with 2 months mice (young), 6m group with 6 months mice (middle-aged) and 15m group with 15 months mice (aged). The phenotype of CD4(+);CD25(+);Foxp3(+); T cells derived from normal murine SPL were analyzed by FCM. Real-time fluorescence quantitative RT-PCR was used to determine the expression levels of Foxp3 mRNA in the spleen PBMC. ELISA was used to detect the concentration of IL-10 and TGF-beta.
  
  
  RESULTS
  The subsets of CD4(+);CD25(+);Foxp3(+); T lymphocytes from murine splenocytes were significantly higher in 15m group than that in 2m group(P<0.01). The expression levels of Foxp3 mRNA in the spleen PBMC were significantly higher in 15m group than that in 2m group(P<0.01).The serum concentration of IL-10 and TGF-beta were significantly higher in 15m group than that in 2m group(P<0.01).There was no correlation between serum concentration of IL-10 and TGF-beta and Foxp3 mRNA expression levels in the spleen PBMC in 15m group.
  
  
  CONCLUSIONS
  The level of the CD4(+);CD25(+);Foxp3(+); T cells and Foxp3 mRNA expression levels were up-regulated and IL-10 and TGF-beta contents were increased in the progress of aging mice. Foxp3 and IL-10 and TGF-beta may be play a part in the process of immunosenescence. Quantitative detection of Foxp3 mRNA by the method of SYBR Green I real-time fluorescence quantitative RT-PCR is stable and reliable.
authors.affiliation: Department of Microbiology and Immunology, Guangdong Medical College, Dongguan 523808, China. zhaoyicomnet@yahoo.com.cn
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhao
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Ji-Zhen
authors.initials: JZ
authors.lastname: Liu
authors.forename: Kang-Rong
authors.initials: KR
authors.lastname: Cai
mesh_terms: D000375:Aging / Q000097:blood / Q000378:metabolism* / Q000473:pathology*; D000818:Animals; D002478:Cells, Cultured; D005434:Flow Cytometry; D051858:Forkhead Transcription Factors / Q000378:metabolism*; D016753:Interleukin-10 / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D020133:Reverse Transcriptase Polymerase Chain Reaction; D050378:T-Lymphocytes, Regulatory; D016212:Transforming Growth Factor beta / Q000097:blood / Q000378:metabolism*
chemical_list: D051858:Forkhead Transcription Factors; D016212:Transforming Growth Factor beta; D016753:Interleukin-10