Modestobacter marinus sp. nov., a psychrotolerant actinobacterium from deep-sea sediment, and emended description of the genus Modestobacter.
The taxonomic status of an actinobacterium that changed colour during growth, strain 42H12-1(T), isolated from deep-sea sediment collected from the Atlantic Ocean, was established using a combination of genotypic and phenotypic data. Strain 42H12-1(T) formed a distinct branch in the 16S rRNA gene phylogenetic tree together with the type strains in the genus Modestobacter. The highest sequence similarity by blast analysis was to Modestobacter versicolor CP153-2(T) (98.5 %) and the second-highest sequence similarity was to Modestobacter multiseptatus AA-826(T) (97.5 %). DNA-DNA relatedness of only 12 % (sd 1.82 %) between strain 42H12-1(T) and M. versicolor DSM 16678(T) differentiated them as members of separate genomic species. Colonies of strain 42H12-1(T) were black on oligotrophic medium, but orange to red, turning black, on copiotrophic medium. The peptidoglycan contained meso-diaminopimelic acid. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and an unknown aminophospholipid. The predominant menaquinone was MK-9(H(4)). The major fatty acids were iso-C(16 : 0) and C(17 : 1)ω8c. The DNA G+C content was 72.3±1 mol%. Strain 42H12-1(T) ( = DSM 45201(T)  = CGMCC 4.5581(T)) is assigned as the type strain of a novel species of the genus Modestobacter, for which the name Modestobacter marinus sp. nov. is proposed.
Xiao
Jing
J
Division of Integrative Environmental Sciences, Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan.|Key Laboratory of Marine Biogenetic Resources, The Third Institute of Oceanography SOA, Xiamen, Fujian, 361005, PR China.
Luo
Yingxue
Y
Key Laboratory of Marine Biogenetic Resources, The Third Institute of Oceanography SOA, Xiamen, Fujian, 361005, PR China.
Xu
Jing
J
Key Laboratory of Marine Biogenetic Resources, The Third Institute of Oceanography SOA, Xiamen, Fujian, 361005, PR China.
Xie
Shujie
S
Key Laboratory of Marine Biogenetic Resources, The Third Institute of Oceanography SOA, Xiamen, Fujian, 361005, PR China.
Xu
Jun
J
State Key Laboratory of Ocean Engineering, Shanghai Jiaotong University, Shanghai 200240, PR China.|College of Life Science & Tech., Shanghai Jiaotong University, Shanghai 200240, PR China.
D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001267:Atlantic Ocean; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D019015:Geologic Sediments / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C073355:menaquinone 9; D003960:Diaminopimelic Acid