title: Tumor growth and angiogenesis is impaired in CIB1 knockout mice.
abstract: BACKGROUND
  Pathological angiogenesis contributes to various ocular, malignant, and inflammatory disorders, emphasizing the need to understand this process more precisely on a molecular level. Previously we found that CIB1, a 22 kDa regulatory protein, plays a critical role in endothelial cell function, angiogenic growth factor-mediated cellular functions, PAK1 activation, MMP-2 expression, and in vivo ischemia-induced angiogenesis. Since pathological angiogenesis is highly dependent on many of these same processes, we hypothesized that CIB1 may also regulate tumor-induced angiogenesis.
  
  
  METHODS
  To test this hypothesis, we allografted either murine B16 melanoma or Lewis lung carcinoma cells into WT and CIB1-KO mice, and monitored tumor growth, morphology, histology, and intra-tumoral microvessel density.
  
  
  RESULTS
  Allografted melanoma tumors that developed in CIB1-KO mice were smaller in volume, had a distinct necrotic appearance, and had significantly less intra-tumoral microvessel density. Similarly, allografted Lewis lung carcinoma tumors in CIB1-KO mice were smaller in volume and mass, and appeared to have decreased perfusion. Intra-tumoral hemorrhage, necrosis, and perivascular fibrosis were also increased in tumors that developed in CIB1-KO mice.
  
  
  CONCLUSIONS
  These findings suggest that, in addition to its other functions, CIB1 plays a critical role in facilitating tumor growth and tumor-induced angiogenesis.
authors.affiliation: Department of Pharmacology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA. parise@med.unc.edu.
authors.forename: Mohamed A
authors.initials: MA
authors.lastname: Zayed
authors.forename: Weiping
authors.initials: W
authors.lastname: Yuan
authors.forename: Dan
authors.initials: D
authors.lastname: Chalothorn
authors.forename: James E
authors.initials: JE
authors.lastname: Faber
authors.forename: Leslie V
authors.initials: LV
authors.lastname: Parise
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