Determination of fumonisins in milled corn grains using HPLC-MS.
A new method for determination of fumonisins in corn samples was developed and validated. The mycotoxins were extracted by a mixture of methanol-acetonitrile-water (1:1:2, v/v/v) and determined on a liquid chromatograph with mass spectrometric detection. The separation was performed on Zorbax XDB-C(18) column (150 x 4.6 mm; 5 microm) with a Metaguard ODS-2 precolumn (30 x 4.6 mm; 5 mum) using gradient elution with mobile phase consisting of acetonitrile and 5 mmol/L ammonium acetate (adjusted by acetic acid to pH 3.0). For detection of (M+H(+)) ions, a quadrupole mass spectrometer in single ion monitoring mode was applied. Developed method showed very good linearity in a tested range of concentration. Detection limit is 62.0 microg FB(1)/kg and 58.5 microg FB(2)/kg of maize grains. Because the detection limits lie under the maximum permitted EU levels, the method is suitable for determination of fumonisins in milled corn grains.
Dohnal
Vlastimil
V
Department of Food Technology, Faculty of Agronomy, Mendel University of Agriculture and Forestry in Brno, Brno, Czech Republic.
Jezková
Alena
A
Polisenská
Ivana
I
Kuca
Kamil
K
D000085:Acetates; D005591:Chemical Fractionation; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D037341:Fumonisins / Q000032:analysis* / Q000737:chemistry; D016014:Linear Models; D013058:Mass Spectrometry / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D003313:Zea mays / Q000737:chemistry*
D000085:Acetates; D037341:Fumonisins; C018824:ammonium acetate