Genetic regulation of pristane-induced oil granuloma responses.
Oil granuloma (OG) induced by intraperitoneal injection of pristane represents a non-infectious granuloma. Oil granuloma has been characterized, but the regulation of its formation still remains unknown. To address this, we injected pristane into various mice deficient for genes including, linker for activation of T cells (LAT), μMT, LTα, TNFα, IL-6. T cell deficient mice (LAT(-/-) ) responded to pristane by developing serosal granuloma and mesenteric granuloma (MG) as in wild type mice. The absence of B cells blocked serosal granuloma (SG) formation and diminished MG development in response to pristane. However, even when a comparable number of B cells were present in the mesentery, the absence of TNFα resulted in similar defects in OG formation after pristane treatment, demonstrating that both B cells and TNFα are very crucial for pristane-induced OG formation. Interestingly, IL-6(-/-) mice had intact MG formation; however, SG organization was impaired. These studies provide insight into granulomateous pathology induced by non-infectious substances for example, biomedical sutures.
Chen
Huaiyong
H
Department of Immunology, Duke University Medical Center, Durham, NC, USA.
Liao
Dongmei
D
Holl
T Matt
TM
Snowden
Pilar
P
Ueda
Yoshihiro
Y
Kelsoe
Garnett
G
D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics* / Q000276:immunology; D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D005260:Female; D005786:Gene Expression Regulation / Q000276:immunology; D006099:Granuloma / Q000139:chemically induced / Q000235:genetics / Q000276:immunology; D007166:Immunosuppressive Agents / Q000633:toxicity; D015850:Interleukin-6 / Q000235:genetics* / Q000276:immunology; D008221:Lymphoid Tissue / Q000276:immunology; D008233:Lymphotoxin-alpha / Q000235:genetics* / Q000276:immunology; D008297:Male; D008565:Membrane Proteins / Q000235:genetics* / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D010750:Phosphoproteins / Q000235:genetics* / Q000276:immunology; D013729:Terpenes / Q000633:toxicity*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics* / Q000276:immunology
D048868:Adaptor Proteins, Signal Transducing; D007166:Immunosuppressive Agents; D015850:Interleukin-6; C491734:Lat protein, mouse; D008233:Lymphotoxin-alpha; D008565:Membrane Proteins; D010750:Phosphoproteins; D013729:Terpenes; D014409:Tumor Necrosis Factor-alpha; C009042:pristane
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