title: Rab11 and its effector Rab coupling protein contribute to the trafficking of beta 1 integrins during axon growth in adult dorsal root ganglion neurons and PC12 cells.
abstract: Integrins play an important part in axon growth, but integrin traffic in neurons is poorly understood. Expression of the tenascin-C-binding integrin alpha9 promotes axon regeneration. We have therefore studied the mechanism by which alpha9 integrin and its partner beta1 are trafficked along axons and at the growth cone using adult DRG neurons and PC12 cells. We have focused on the small GTPase Rab11 and its effector Rab coupling protein (RCP), as they are involved in the long-range trafficking of beta1 integrins in other cells. Rab11 colocalizes with alpha9 and other alpha integrins and with beta1 integrin in growth cones and axons, and immunopurified Rab11 vesicles contain alpha9 and beta1. Endocytosed beta1 integrins traffic via Rab11. However, Rab11 vesicles in axons are generally static, and alpha9 integrins undergo bouts of movement during which they leave the Rab11 compartment. In growth cones, alpha9 and beta1 overlap with RCP, particularly at the growth cone periphery. We show that beta1 integrin trafficking during neurite outgrowth involves Rab11 and RCP, and that manipulation of these molecules alters surface integrin levels and axon growth, and can be used to enhance alpha9 integrin-dependent neurite outgrowth. Our data suggest that manipulation of trafficking via Rab11 and RCP could be a useful strategy for promoting integrin-dependent axonal regeneration.
authors.affiliation: Cambridge Centre for Brain Repair, Department of Clinical Neurosciences, University of Cambridge, Cambridge, United Kingdom.
authors.forename: Richard
authors.initials: R
authors.lastname: Eva
authors.forename: Elisa
authors.initials: E
authors.lastname: Dassie
authors.forename: Patrick T
authors.initials: PT
authors.lastname: Caswell
authors.forename: Gunnar
authors.initials: G
authors.lastname: Dick
authors.forename: Charles
authors.initials: C
authors.lastname: ffrench-Constant
authors.forename: Jim C
authors.initials: JC
authors.lastname: Norman
authors.forename: James W
authors.initials: JW
authors.lastname: Fawcett
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism / Q000502:physiology*; D000367:Age Factors; D000818:Animals; D001369:Axons / Q000502:physiology*; D002478:Cells, Cultured; D005727:Ganglia, Spinal / Q000166:cytology* / Q000378:metabolism / Q000502:physiology*; D020439:Growth Cones / Q000378:metabolism / Q000502:physiology; D006801:Humans; D019012:Integrin beta1 / Q000378:metabolism*; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism / Q000502:physiology*; D009474:Neurons / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D016716:PC12 Cells; D021381:Protein Transport / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D020691:rab GTP-Binding Proteins / Q000378:metabolism / Q000502:physiology*; D000099193:rab11 GTP-Binding Proteins
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D019012:Integrin beta1; D008565:Membrane Proteins; D020691:rab GTP-Binding Proteins; D000099193:rab11 GTP-Binding Proteins; C488966:RAB11FIP1 protein, human
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