title: Laser capture microdissection in the tissue biorepository.
abstract: An important need of many cancer research projects is the availability of high-quality, appropriately selected tissue. Tissue biorepositories are organized to collect, process, store, and distribute samples of tumor and normal tissue for further use in fundamental and translational cancer research. This, in turn, provides investigators with an invaluable resource of appropriately examined and characterized tissue specimens and linked patient information. Human tissues, in particular, tumor tissues, are complex structures composed of heterogeneous mixtures of morphologically and functionally distinct cell types. It is essential to analyze specific cell types to identify and define accurately the biologically important processes in pathologic lesions. Laser capture microdissection (LCM) is state-of-the-art technology that provides the scientific community with a rapid and reliable method to isolate a homogeneous population of cells from heterogeneous tissue specimens, thus providing investigators with the ability to analyze DNA, RNA, and protein accurately from pure populations of cells. This is particularly well-suited for tumor cell isolation, which can be captured from complex tissue samples. The combination of LCM and a tissue biorepository offers a comprehensive means by which researchers can use valuable human biospecimens and cutting-edge technology to facilitate basic, translational, and clinical research. This review provides an overview of LCM technology with an emphasis on the applications of LCM in the setting of a tissue biorepository, based on the author's extensive experience in LCM procedures acquired at Fox Chase Cancer Center and Hollings Cancer Center.
authors.affiliation: Tissue Biorepository, Hollings Cancer Center, Medical University of South Carolina, Charleston, South Carolina 29425, USA. liuan@musc.edu
authors.forename: Angen
authors.initials: A
authors.lastname: Liu
mesh_terms: D004247:DNA / Q000032:analysis; D006801:Humans; D007834:Lasers; D042282:Microdissection / Q000379:methods*; D011506:Proteins / Q000032:analysis; D012313:RNA / Q000032:analysis; D013048:Specimen Handling
chemical_list: D011506:Proteins; D012313:RNA; D004247:DNA
references.citation: Anal Chem. 2005 Feb 1;77(3):742-9
references.pmid: 15679339
references.citation: Biol Psychiatry. 2004 Jan 15;55(2):107-11
references.pmid: 14732588
references.citation: Cancer Genet Cytogenet. 2007 May;175(1):19-25
references.pmid: 17498553
references.citation: World J Gastroenterol. 2006 Nov 21;12(43):6949-54
references.pmid: 17109515
references.citation: Croat Med J. 2005 Aug;46(4):549-55
references.pmid: 16100757
references.citation: Methods Mol Biol. 2006;319:231-6
references.pmid: 16719358
references.citation: Methods Mol Biol. 2006;319:213-29
references.pmid: 16719357
references.citation: Pathobiology. 2000;68(4-5):196-201
references.pmid: 11279346
references.citation: J Cutan Pathol. 2003 Apr;30(4):265-70
references.pmid: 12680959
references.citation: Biotechniques. 2002 Jul;33(1):176-9
references.pmid: 12139243
references.citation: Methods Enzymol. 2002;356:334-43
references.pmid: 12418212
references.citation: J Mol Diagn. 2007 Feb;9(1):70-9
references.pmid: 17251338
references.citation: Histochem Cell Biol. 2007 Feb;127(2):215-9
references.pmid: 17093949
references.citation: Anticancer Res. 2006 May-Jun;26(3A):2069-74
references.pmid: 16827146
references.citation: Pathobiology. 2000;68(4-5):209-14
references.pmid: 11279348
references.citation: Cytometry A. 2006 Sep 1;69(9):947-60
references.pmid: 16969815
references.citation: Int J Legal Med. 2009 Mar;123(2):169-75
references.pmid: 18661142
references.citation: Int J Mol Med. 2005 May;15(5):763-9
references.pmid: 15806296
references.citation: Expert Rev Mol Diagn. 2007 Sep;7(5):647-57
references.pmid: 17892370
references.citation: Methods Mol Biol. 2005;293:113-23
references.pmid: 16028415
references.citation: Pathobiology. 2000;68(4-5):232-6
references.pmid: 11279352
references.citation: Hepatology. 2002 Jan;35(1):110-6
references.pmid: 11786966
references.citation: Acta Histochem. 2007;109(3):171-6
references.pmid: 17462720
references.citation: Brain Pathol. 2004 Apr;14(2):175-82
references.pmid: 15193030
references.citation: Forensic Sci Int. 2007 Dec 20;173(2-3):93-6
references.pmid: 17459628
references.citation: Chest. 2005 Oct;128(4):2876-81
references.pmid: 16236965
references.citation: Science. 1996 Nov 8;274(5289):998-1001
references.pmid: 8875945
references.citation: Oncol Rep. 2008 Apr;19(4):999-1005
references.pmid: 18357388
references.citation: J Forensic Sci. 1998 Jan;43(1):114-8
references.pmid: 9456531
references.citation: Forensic Sci Int. 2007 May 3;168(1):42-56
references.pmid: 16899347
references.citation: Forensic Sci Int. 2004 Dec 2;146 Suppl:S151-3
references.pmid: 15639564
references.citation: Adv Exp Med Biol. 2007;593:54-65
references.pmid: 17265716
references.citation: Clin Lymphoma Myeloma. 2009 Jun;9(3):206-16
references.pmid: 19525189
references.citation: Int J Legal Med. 2005 May;119(3):158-63
references.pmid: 15565297
references.citation: J Histochem Cytochem. 2005 Mar;53(3):409-12
references.pmid: 15750030
references.citation: Methods Mol Med. 2003;86:237-55
references.pmid: 12886773
references.citation: Forensic Sci Int. 2002 Oct 9;129(3):158-67
references.pmid: 12372686
references.citation: Ultrastruct Pathol. 2006 May-Jun;30(3):221-8
references.pmid: 16825124
references.citation: Methods Mol Med. 2007;132:11-8
references.pmid: 17876072
references.citation: Exp Cell Res. 1986 Nov;167(1):262-5
references.pmid: 3758206