title: Effect of enzyme modification by well-defined multi-armed poly(ethylene glycol) synthesized using polyamidoamine dendron.
abstract: Egg white lysozyme was chemically modified by PEGylated PAMAM 1st, 2nd and 3rd generation dendrons, which had 2, 4 and 8 PEG arms, respectively. The number of PEG chains introduced to the lysozyme molecule drastically increased with an increase in dendron generation, although the number of PEGylated PAMAM dendrons introduced decreased due to steric repulsion. The lytic activity of lysozyme to Micrococcus luteus cells was effectively inhibited by conjugating PEGylated PAMAM dendron to the lysozyme, indicating steric stabilization of PEG chains at the surface of lysozyme molecule. In addition, the enzymatic reaction of the lysozyme with oligosaccharide substrate was apparently accelerated by a substrate condensation effect due to the multi-armed structure of PEG.
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Osaka Prefecture University, Gakuen-cho, Naka-ku, Sakai, Japan. harada@chem.osakafu-u.ac.jp
authors.forename: Atsushi
authors.initials: A
authors.lastname: Harada
authors.forename: Takeshi
authors.initials: T
authors.lastname: Kato
authors.forename: Akifumi
authors.initials: A
authors.lastname: Kawamura
authors.forename: Chie
authors.initials: C
authors.lastname: Kojima
authors.forename: Kenji
authors.initials: K
authors.lastname: Kono
mesh_terms: D050091:Dendrimers / Q000737:chemistry*; D004527:Egg Proteins / Q000378:metabolism; D016982:Micrococcus luteus / Q000166:cytology / Q000378:metabolism; D008970:Molecular Weight; D009113:Muramidase / Q000378:metabolism*; D011092:Polyethylene Glycols / Q000138:chemical synthesis* / Q000737:chemistry* / Q000378:metabolism
chemical_list: D050091:Dendrimers; D004527:Egg Proteins; C104700:PAMAM Starburst; D011092:Polyethylene Glycols; D009113:Muramidase