Fine-tuning DNA/albumin polyelectrolyte interactions to produce the efficient transfection agent cBSA-147.
We present the preparation and isolation of different chemically modified BSA species with varying numbers of primary amino groups at the surface. Highly cationic albumin proteins with increased numbers of amino groups were achieved and complex formation with plasmid DNA was carefully investigated. We compare the transfection results, polyelectrolyte complexes morphologies with their impact on complex stabilities, cytotoxicities and DNA accessibility. This knowledge-driven approach led to the identification of the efficient non-viral DNA delivery agent cBSA-147, which showed high transfection efficacies and stability.
Eisele
Klaus
K
Max Planck Institute for Polymer Research, Ackermannweg 10, 55128 Mainz, Germany.
Gropeanu
Radu A
RA
Zehendner
Christoph M
CM
Rouhanipour
Ali
A
Ramanathan
Arvind
A
Mihov
George
G
Koynov
Kalojan
K
Kuhlmann
Christoph R W
CR
Vasudevan
Subhash G
SG
Luhmann
Heiko J
HJ
Weil
Tanja
T
D000818:Animals; D016923:Cell Death / Q000187:drug effects; D002966:Clathrin / Q000378:metabolism; D004247:DNA / Q000378:metabolism*; D004573:Electrolytes / Q000378:metabolism*; D011992:Endosomes / Q000187:drug effects / Q000378:metabolism; D004996:Ethidium / Q000737:chemistry; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight; D010316:Particle Size; D010957:Plasmids / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000378:metabolism* / Q000633:toxicity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D055672:Static Electricity; D034421:Sus scrofa; D013816:Thermodynamics; D014162:Transfection / Q000379:methods*
D002966:Clathrin; D004573:Electrolytes; D049452:Green Fluorescent Proteins; D012710:Serum Albumin, Bovine; D004247:DNA; D004996:Ethidium