Dynamic organization of chromatin assembly and transcription factories in living cells.
The interphase nucleus is an active organelle involved in processing genetic information. In higher order eukaryotes, information control is compartmentalized - for example at the scale of inter-chromosome territories and nuclear bodies. Regulatory proteins, nuclear bodies and chromatin assembly are found to be highly dynamic within the nucleus of primary cells and through cellular differentiation programs. In this chapter we describe live-cell fluorescence based techniques and single particle tracking analysis, to probe the spatio-temporal dimension in nuclear function.
Sinha
Bidisha
B
National Centre for Biological Sciences, Tata Institute of Fundamental Research, Bellary Road, Bangalore 560065, India.
Bhattacharya
Dipanjan
D
Sinha
Deepak Kumar
DK
Talwar
Shefali
S
Maharana
Shovamayee
S
Gupta
Soumya
S
Shivashankar
G V
GV
D000818:Animals; D018929:Cell Culture Techniques; D002468:Cell Physiological Phenomena / Q000235:genetics*; D002477:Cells / Q000378:metabolism*; D002478:Cells, Cultured; D042002:Chromatin Assembly and Disassembly / Q000502:physiology*; D036681:Fluorescence Recovery After Photobleaching / Q000379:methods; D006801:Humans; D007700:Kinetics; D008962:Models, Theoretical; D013050:Spectrometry, Fluorescence / Q000379:methods; D014158:Transcription, Genetic / Q000502:physiology*