title: Crystallization and preliminary crystallographic characterization of the iron-regulated outer membrane lipoprotein FrpD from Neisseria meningitidis.
abstract: Fe-regulated protein D (FrpD) is a Neisseria meningitidis outer membrane lipoprotein that may be involved in the anchoring of the secreted repeat in toxins (RTX) protein FrpC to the outer bacterial membrane. However, the function and biological roles of the FrpD and FrpC proteins remain unknown. Native and selenomethionine-substituted variants of recombinant FrpD43-271 protein were crystallized using the sitting-drop vapour-diffusion method. Diffraction data were collected to a resolution of 2.25 A for native FrpD43-271 protein and to a resolution of 2.00 A for selenomethionine-substituted FrpD43-271 (SeMet FrpD43-271) protein. The crystals of native FrpD43-271 protein belonged to the hexagonal space group P6(2) or P6(4), while the crystals of SeMet FrpD43-271 protein belonged to the primitive orthorhombic space group P2(1)2(1)2(1).
authors.affiliation: Institute of Physical Biology, University of South Bohemia Ceske Budejovice, Zamek 136, 373 33 Nove Hrady, Czech Republic.
authors.forename: Ekaterina
authors.initials: E
authors.lastname: Sviridova
authors.forename: Ladislav
authors.initials: L
authors.lastname: Bumba
authors.forename: Pavlina
authors.initials: P
authors.lastname: Rezacova
authors.forename: Katerina
authors.initials: K
authors.lastname: Prochazkova
authors.forename: Daniel
authors.initials: D
authors.lastname: Kavan
authors.forename: Karel
authors.initials: K
authors.lastname: Bezouska
authors.forename: Michal
authors.initials: M
authors.lastname: Kuty
authors.forename: Peter
authors.initials: P
authors.lastname: Sebo
authors.forename: Ivana
authors.initials: I
authors.lastname: Kuta Smatanova
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D008074:Lipoproteins / Q000737:chemistry*; D009345:Neisseria meningitidis / Q000737:chemistry*
chemical_list: D001425:Bacterial Outer Membrane Proteins; D008074:Lipoproteins
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