Simian immunodeficiency virus-specific CD4+ T cells from successful vaccinees target the SIV Gag capsid.
We recently demonstrated that vaccinated rhesus macaques controlled viral replication of a heterologous SIV challenge. Here, we analyzed anamnestic SIV-specific CD4+ T-cell responses expanding immediately after challenge and show that successful vaccinees consistently targeted a short region of the Gag-p27 Capsid (amino acids 249-291). We have also defined the major histocompatibility complex class II (MHC-II) restricting alleles for several of these responses and show that DQ-restricted CD4+ T-cells depend on unique combinations of both the DQA and DQB alleles. Analysis of SIV-specific CD4+ T-cell responses elicited by a successful vaccine may have important implications in the understanding of vaccine design.
Giraldo-Vela
Juan P
JP
Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, WI 53711, USA.
Bean
Alex T
AT
Rudersdorf
Richard
R
Wallace
Lyle T
LT
Loffredo
John T
JT
Erickson
Priscilla
P
Wilson
Nancy A
NA
Watkins
David I
DI
D000595:Amino Acid Sequence; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D000939:Epitopes / Q000276:immunology; D015683:Gene Products, gag / Q000276:immunology*; D005802:Genes, MHC Class II; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D007156:Immunologic Memory; D008253:Macaca mulatta / Q000235:genetics / Q000276:immunology*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000276:immunology; D012191:Retroviridae Proteins / Q000276:immunology; D018449:SAIDS Vaccines / Q000276:immunology*; D016097:Simian Acquired Immunodeficiency Syndrome / Q000276:immunology / Q000517:prevention & control; D015302:Simian Immunodeficiency Virus / Q000276:immunology* / Q000302:isolation & purification / Q000502:physiology; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D014611:Vaccination; D019562:Viral Load; D014766:Viremia / Q000276:immunology / Q000517:prevention & control; D014779:Virus Replication
D000939:Epitopes; C066594:Gag protein p27, Simian immunodeficiency virus; D015683:Gene Products, gag; D000949:Histocompatibility Antigens Class II; D010446:Peptide Fragments; D012191:Retroviridae Proteins; D018449:SAIDS Vaccines
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