Crystallization and preliminary X-ray analysis of formate oxidase, an enzyme of the glucose-methanol-choline oxidoreductase family.
Formate oxidase (FOD), which catalyzes the oxidation of formate to yield carbon dioxide and hydrogen peroxide, belongs to the glucose-methanol-choline oxidoreductase (GMCO) family. FOD from Aspergillus oryzae RIB40, which has a modified FAD as a cofactor, was crystallized at 293 K by the hanging-drop vapour-diffusion method. The crystal was orthorhombic and belonged to space group C222(1). Diffraction data were collected from a single crystal to 2.4 A resolution.
Maeda
Yoshifumi
Y
Department of Applied Chemistry and Biotechnology, Graduate School of Engineering, University of Fukui, 9-1 Bunkyo 3-chome, Fukui-shi 910-8507, Japan.
Doubayashi
Daiju
D
Ootake
Takumi
T
Oki
Masaya
M
Mikami
Bunzo
B
Uchida
Hiroyuki
H
D000595:Amino Acid Sequence; D001236:Aspergillus oryzae / Q000201:enzymology*; D003460:Crystallization; D018360:Crystallography, X-Ray; D005948:Glucose Dehydrogenases / Q000737:chemistry*; D008969:Molecular Sequence Data; D016415:Sequence Alignment
D005948:Glucose Dehydrogenases
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