Different effects of implanting vascular bundles and sensory nerve tracts on the expression of neuropeptide receptors in tissue-engineered bone in vivo.
We investigated whether implantation of vascular bundles or sensory nerves affected the expression of calcitonin gene-related peptide type I receptor (CGRP1R) and neuropeptide Y1 receptor (NPY1R) in tissue-engineered bone. We implanted osteogenically induced bone marrow mesenchymal stem cells (BMSCs) with β-tricalcium phosphate (β-TCP) as the scaffold material either with sensory nerve tracts (group I, n = 18), vascular bundles (group II, n = 18) or alone (group III, n = 18) to repair a 1.2 cm femur defect in the rabbit. Better osteogenesis was observed by x-ray and histology in groups I and II than in group III at 4, 8 and 12 weeks. Within the new bone, the mRNA levels of the two neuropeptide receptors determined by real-time PCR increased through week 8, and then gradually decreased (P < 0.05). Expression of the neuropeptide receptors determined by immunohistochemistry was lowest at 4 weeks (P < 0.05) and was higher in group II than in group I (P < 0.05). Expression was significantly higher in groups I and II than in group III at all time points. We conclude that implanting vascular bundles into tissue-engineered bone can significantly improve the early expression of CGRP1R and NPY1R. In contrast, implantation of sensory nerves did not show the same dramatic effect as implantation of vascular bundles.
Chen
Si-Yuan
SY
Department of Orthopaedic & Trauma, Nanfang Hospital, Southern Medical University, Guangzhou 510515, People's Republic of China.
Qin
Jun-Jun
JJ
Wang
Le
L
Mu
Tian-Wang
TW
Jin
Dan
D
Jiang
Shan
S
Zhao
Pei-Ran
PR
Pei
Guo-Xian
GX
D000818:Animals; D001808:Blood Vessels / Q000637:transplantation*; D001842:Bone and Bones / Q000601:surgery*; D002130:Calcium Phosphates / Q000737:chemistry; D005240:Feasibility Studies; D005269:Femur / Q000000981:diagnostic imaging / Q000293:injuries; D007150:Immunohistochemistry; D009417:Nerve Tissue / Q000637:transplantation*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism; D011817:Rabbits; D011859:Radiography; D018013:Receptors, Neuropeptide / Q000378:metabolism*; D013997:Time Factors; D023822:Tissue Engineering
D002130:Calcium Phosphates; D012333:RNA, Messenger; D018013:Receptors, Neuropeptide; C485817:beta-tricalcium phosphate