title: Halorussus rarus gen. nov., sp. nov., a new member of the family Halobacteriaceae isolated from a marine solar saltern.
abstract: Two halophilic archaeal strains TBN4(T) and TBN5 were isolated from Taibei marine solar saltern in Jiangsu, China. Both strains showed light red-pigmented colonies and their cells were rod, motile and Gram-stain-negative. They were able to grow at 25-50°C (optimum 37°C), at 1.4-4.3 M NaCl (optimum 2.1 M NaCl), at 0-1.0 M MgCl(2) (optimum 0.005 M MgCl(2)) and at pH 6.0-9.0 (optimum pH 7.0). Their cells lyse in distilled water and minimal NaCl concentration to prevent cell lysis is 8% (w/v). The major polar lipids of the two strains were PG (phosphatidylglycerol), PGP-Me (phosphatidylglycerol phosphate methyl ester), PGS (phosphatidylglycerol sulfate) and five glycolipids chromatographically identical to S-TGD-1 (sulfated galactosyl mannosyl glucosyl diether), S-DGD-1 (sulfated mannosyl glucosyl diether), TGD-1 (galactosyl mannosyl glucosyl diether), DGD-1 (mannosyl glucosyl diether) and DGD-2 (an unknown diglycosyl diether). Phylogenetic analysis revealed that TBN4(T) and strain TBN5 formed a distinct clade with genus Haladaptatus (showing 90.0-90.9% 16S rRNA gene similarities). The DNA G + C content of strain TBN4(T) and strain TBN5 are 66.1 and 65.4 mol%, respectively. The DNA-DNA hybridization value between strain TBN4(T) and strain TBN5 was 94.3%. The phenotypic, chemotaxonomic and phylogenetic properties suggest that strain TBN4(T) and strain TBN5 represent a novel species in a new genus within the family Halobacteriaceae, for which the name Halorussus rarus gen. nov., sp. nov. is proposed. The type strain is TBN4(T) (=CGMCC 1.10122(T) = JCM 16429(T)).
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Jingkou District, Zhenjiang, 212013, People's Republic of China. cuihenglin@sohu.com
authors.forename: Heng-Lin
authors.initials: HL
authors.lastname: Cui
authors.forename: Xia
authors.initials: X
authors.lastname: Gao
authors.forename: Xin
authors.initials: X
authors.lastname: Yang
authors.forename: Xue-Wei
authors.initials: XW
authors.lastname: Xu
mesh_terms: D000818:Animals; D001482:Base Composition / Q000235:genetics; D058448:Biota; D002681:China; D019641:DNA, Archaeal / Q000502:physiology*; D005838:Genotype; D006216:Halobacteriaceae / Q000737:chemistry / Q000302:isolation & purification / Q000502:physiology; D006824:Hybridization, Genetic / Q000502:physiology; D010641:Phenotype; D010802:Phylogeny; D010860:Pigments, Biological / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D055049:Salt Tolerance / Q000235:genetics / Q000502:physiology; D012965:Sodium Chloride / Q000737:chemistry
chemical_list: D019641:DNA, Archaeal; D010860:Pigments, Biological; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride
references.citation: Int J Syst Evol Microbiol. 2007 Apr;57(Pt 4):717-720
references.pmid: 17392193
references.citation: Extremophiles. 2009 Jan;13(1):31-7
references.pmid: 18836684
references.citation: J Bacteriol. 1955 Oct;70(4):484-5
references.pmid: 13263323
references.citation: Int J Syst Evol Microbiol. 2007 Oct;57(Pt 10):2204-2206
references.pmid: 17911283
references.citation: Mol Biol Evol. 1987 Jul;4(4):406-25
references.pmid: 3447015
references.citation: Appl Microbiol. 1972 Sep;24(3):516-7
references.pmid: 4562486
references.citation: Int J Syst Evol Microbiol. 2007 Mar;57(Pt 3):600-604
references.pmid: 17329792
references.citation: Int J Syst Evol Microbiol. 2005 Nov;55(Pt 6):2501-2505
references.pmid: 16280517
references.citation: Int J Syst Evol Microbiol. 2007 Jan;57(Pt 1):19-24
references.pmid: 17220434
references.citation: Int J Syst Evol Microbiol. 2010 May;60(Pt 5):1085-1089
references.pmid: 19666806
references.citation: Int J Syst Evol Microbiol. 2007 Oct;57(Pt 10):2199-2203
references.pmid: 17911282
references.citation: Brief Bioinform. 2008 Jul;9(4):299-306
references.pmid: 18417537
references.citation: Can J Microbiol. 1978 Jun;24(6):710-5
references.pmid: 667737
references.citation: Syst Appl Microbiol. 1983;4(2):184-92
references.pmid: 23194591
references.citation: Int J Syst Evol Microbiol. 2006 Apr;56(Pt 4):765-770
references.pmid: 16585691
references.citation: Int J Syst Evol Microbiol. 2008 Apr;58(Pt 4):856-60
references.pmid: 18398182
references.citation: J Mol Evol. 1981;17(6):368-76
references.pmid: 7288891
references.citation: Int J Syst Evol Microbiol. 2009 Mar;59(Pt 3):637-42
references.pmid: 19244452
references.citation: Environ Microbiol. 2004 Jun;6(6):591-5
references.pmid: 15142247
references.citation: Int J Syst Evol Microbiol. 2000 May;50 Pt 3:1297-1303
references.pmid: 10843075
references.citation: Int J Syst Evol Microbiol. 2002 Mar;52(Pt 2):485-91
references.pmid: 11931160
references.citation: J Bacteriol. 1959 Jan;77(1):60-4
references.pmid: 13620650
references.citation: Int J Syst Evol Microbiol. 2008 Dec;58(Pt 12):2880-4
references.pmid: 19060076
references.citation: Int J Syst Evol Microbiol. 2010 May;60(Pt 5):1011-1012
references.pmid: 20458121
references.citation: Int J Syst Evol Microbiol. 2007 Oct;57(Pt 10):2296-2298
references.pmid: 17911300
references.citation: Eur J Biochem. 1970 Jan;12(1):133-42
references.pmid: 4984993
references.citation: Int J Syst Bacteriol. 1999 Jan;49 Pt 1:261-6
references.pmid: 10028271
references.citation: Int J Syst Evol Microbiol. 2007 Oct;57(Pt 10):2259-2261
references.pmid: 17911292
references.citation: Int J Syst Evol Microbiol. 2008 Jan;58(Pt 1):215-20
references.pmid: 18175711