title: The effect of PEG-coated gold nanoparticles on the anti-proliferative potential of Specific Nutrient Synergy.
abstract: The role of PEG-coated gold nanoparticles (Au NPs) on the anti-proliferative effect of Specific Nutrient Synergy (SNS) on HTLV-1 infected (C91-PL and HuT-102) and non-infected (CEM and Jurkat) malignant T-lymphocytes cells, was investigated. When PEG-coated Au NPs (of different molecular weights) were added alone, there was no effect on either viability or proliferation of the leukemic cell lines studied. Treatment of cells with SNS and PEG (5 or 10 kDa) coated Au NP reduced significantly the proliferation in all cell lines tested; this reached more than 50% reduction as compared to the control for cells treated for 96 h. Data showed that the best anti-proliferative effect was obtained using SNS and Au NP coated with PEG of molecular weights of 5 and 10 kDa with almost no effect of PEG of lower molecular weights (0.75 and 2 kDa) or higher ones (20 kDa). This was true as well for HTLV-1 infected as for non-infected malignant T-lymphocytes. Electron microscopy results showed uptake of the gold particles to Jurkat cells. All described effects are specific to leukemia cell lines, and no effects were observed with freshly activated human mononuclear lymphocytes as control.
authors.affiliation: Dr. Rath Research Institute, Santa Clara, CA, USA.
authors.forename: Steve
authors.initials: S
authors.lastname: Harakeh
authors.forename: Roula M
authors.initials: RM
authors.lastname: Abdel-Massih
authors.forename: Pilar Rivera
authors.initials: PR
authors.lastname: Gil
authors.forename: Ralph A
authors.initials: RA
authors.lastname: Sperling
authors.forename: Andreas
authors.initials: A
authors.lastname: Meinhardt
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Niedwiecki
authors.forename: Matthias
authors.initials: M
authors.lastname: Rath
authors.forename: Wolfgang J
authors.initials: WJ
authors.lastname: Parak
authors.forename: Elias
authors.initials: E
authors.lastname: Baydoun
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival; D019587:Dietary Supplements; D004357:Drug Synergism; D006046:Gold / Q000737:chemistry / Q000494:pharmacology*; D015368:Human T-lymphotropic virus 1 / Q000254:growth & development; D006801:Humans; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry*; D013601:T-Lymphocytes / Q000187:drug effects / Q000648:ultrastructure / Q000821:virology
chemical_list: D011092:Polyethylene Glycols; D006046:Gold