title: Vaccination with a modified vaccinia virus Ankara (MVA)-vectored HIV-1 immunogen induces modest vector-specific T cell responses in human subjects.
abstract: We investigated whether vaccination of healthy HIV-seronegative and HIV-1-seropositive antiretroviral therapy-treated subjects with recombinant modified vaccinia virus Ankara expressing an HIV-1 immunogen (MVA.HIVA) induced MVA-specific T cell responses. Using IFN-γ Elispot assays, we observed new or increased responses to MVA virus in 52% of HIV-seronegative subjects and 93% HIV-1 seropositive subjects; MVA-specific T cell frequencies were generally low and correlated poorly with T cell responses to the HIV-1 immunogen. In two vaccinees, responses were mapped to CD8+ T cell epitopes present in replication-competent vaccinia virus. These data support further evaluation of MVA as a viral vector for HIV-1 immunogens.
authors.affiliation: MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Oxford OX3 9DS, United Kingdom.
authors.forename: Sarah
authors.initials: S
authors.lastname: Howles
authors.forename: Ana
authors.initials: A
authors.lastname: Guimarães-Walker
authors.forename: Hongbing
authors.initials: H
authors.lastname: Yang
authors.forename: Gemma
authors.initials: G
authors.lastname: Hancock
authors.forename: Katalin
authors.initials: K
authors.lastname: di Gleria
authors.forename: Tony
authors.initials: T
authors.lastname: Tarragona-Fiol
authors.forename: Peter
authors.initials: P
authors.lastname: Hayes
authors.forename: Jill
authors.initials: J
authors.lastname: Gilmour
authors.forename: Anne
authors.initials: A
authors.lastname: Bridgeman
authors.forename: Tomáš
authors.initials: T
authors.lastname: Hanke
authors.forename: Andrew
authors.initials: A
authors.lastname: McMichael
authors.forename: Lucy
authors.initials: L
authors.lastname: Dorrell
mesh_terms: D016915:AIDS Vaccines / Q000276:immunology*; D000293:Adolescent; D000328:Adult; D023241:Antiretroviral Therapy, Highly Active; D018984:Epitopes, T-Lymphocyte / Q000276:immunology; D005822:Genetic Vectors; D015658:HIV Infections / Q000188:drug therapy / Q000276:immunology / Q000517:prevention & control*; D018023:HIV Seronegativity; D015497:HIV-1 / Q000276:immunology; D006801:Humans; D007111:Immunity, Cellular; D007117:Immunization, Secondary; D007371:Interferon-gamma / Q000276:immunology; D008875:Middle Aged; D013601:T-Lymphocytes / Q000276:immunology*; D014616:Vaccinia virus / Q000276:immunology*; D055815:Young Adult
chemical_list: D016915:AIDS Vaccines; D018984:Epitopes, T-Lymphocyte; D007371:Interferon-gamma