title: Elastin-like polypeptides as a purification tag for recombinant proteins.
abstract: This unit presents a recombinant protein purification method that employs an elastin-like polypeptide (ELP) as a purification tag. ELPs undergo a sharp and reversible phase transition when heated above their lower critical solution temperature. ELPs retain this behavior when they are fused to a protein, and thereby provide a simple method to isolate a recombinant ELP fusion protein from cell contaminants by cycling the solution through the insoluble and soluble phase of the ELP fusion protein using a procedure that is termed Inverse Transition Cycling. This method does not require the use of chromatography, so it is cost-effective, easy to scale up, and easy to multiplex.
authors.affiliation: Duke University, Durham, North Carolina.
authors.forename: Wafa
authors.initials: W
authors.lastname: Hassouneh
authors.affiliation: Duke University, Durham, North Carolina.
authors.forename: Trine
authors.initials: T
authors.lastname: Christensen
authors.affiliation: Duke University, Durham, North Carolina.
authors.forename: Ashutosh
authors.initials: A
authors.lastname: Chilkoti
mesh_terms: D002846:Chromatography, Affinity; D004549:Elastin / Q000737:chemistry* / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism; D010316:Particle Size; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000302:isolation & purification*; D013696:Temperature
chemical_list: D010455:Peptides; D011993:Recombinant Fusion Proteins; D004549:Elastin
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