Migratory route of Strongyloides venezuelensis in Lewis rats: comparison of histological analyses and PCR.
Strongyloides venezuelensis is a parasitic nematode that has been used as a model to study human and animal strongyloidiasis. In this study, we compared the sensitivity between traditional methodologies and PCR assay to characterize the dynamics of S. venezuelensis infection and its migration route in Lewis rats subcutaneously infected with 4000 L3. The dynamics of the infection was determined by counting the number of eggs and by detecting parasite deoxyribonucleic acid in faeces samples. Both techniques similarly detected the infection at day 6 after larvae inoculation. However, PCR performed with the genus primer showed higher sensitivity during the recovery phase. Histological analysis and PCR assay were then used to follow parasite tissue migration. S. venezuelensis migration route included the muscular fibers below the skin, the pulmonary alveoli and the small intestine vilosities. The sensitivity of these two techniques to detect parasite's presence in these tissues was statistically similar.
Marra
Nelson Mendes
NM
Department of Parasitology, Biosciences Institute, Univ Estadual Paulista, Botucatu, São Paulo, Brazil.
Chiuso-Minicucci
Fernanda
F
Machado
Gabriel Capella
GC
Zorzella-Pezavento
Sofia Fernanda Gonçalves
SF
França
Thaís Graziela Donegá
TG
Ishikawa
Larissa Lumi Watanabe
LL
Amarante
Alessandro F T
AF
Sartori
Alexandrina
A
Amarante
Mônica R V
MR
D000818:Animals; D001921:Brain / Q000469:parasitology; D018509:DNA, Helminth / Q000032:analysis; D005243:Feces / Q000469:parasitology; D005260:Female; D007421:Intestine, Small / Q000469:parasitology; D008168:Lung / Q000469:parasitology; D008297:Male; D018482:Muscle, Skeletal / Q000469:parasitology; D010270:Parasite Egg Count; D016133:Polymerase Chain Reaction; D051381:Rats; D011917:Rats, Inbred Lew; D012680:Sensitivity and Specificity; D012867:Skin / Q000469:parasitology; D013321:Strongyloides / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D013322:Strongyloidiasis / Q000469:parasitology*; D013997:Time Factors; D014781:Viscera / Q000469:parasitology
D018509:DNA, Helminth