Anti-allergic, anti-inflammatory and anti-lipidperoxidant effects of Cassia occidentalis Linn.
Cassia occidentalis Linn. mast cell degranulation at a dose of 250 mg/kg, showed dose dependent stabilizing activity towards human RBC, with is widely used in traditional medicine of India to treat a number of clinical conditions including allergy and inflammatory manifestations. In the present study anti-allergic, anti-inflammatory and anti-oxidant properties of C. occidentalis whole plant ethanolic extract (CO) was investigated. Effects of CO on rat mast cell degranulation inhibition and human red blood cell (HRBC) membrane stabilization were studied in vitro following standard methods. The anti lipidperoxidant effects of CO were also studied in vitro. Effect of CO on carrageenan-induced mouse paw oedema inhibition was also assessed. CO significantly decreased maximum protection of 80.8% at 15 microg/ml. The extract also caused significant reduction in malondialdehyde (MDA) levels of murine hepatic microsomes at 100 microg/ml (56%) and significantly reduced carrageenan induced inflammation in mice at a dose of 250 mg/kg. Results of the present study indicated that CO inhibited mast cell degranulation, stabilized HRBC membrane thereby alleviating immediate hypersensitivity besides showing anti oxidant activity.
Sreejith
G
G
Malankara Catholic College, Mariagiri, Kaliakkavilai 629 153, India.
Latha
P G
PG
Shine
V J
VJ
Anuja
G I
GI
Suja
S R
SR
Sini
S
S
Shyama
S
S
Pradeep
S
S
Shikha
P
P
Rajasekharan
S
S
D000818:Animals; D018926:Anti-Allergic Agents / Q000302:isolation & purification / Q000627:therapeutic use* / Q000633:toxicity; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000302:isolation & purification / Q000627:therapeutic use* / Q000633:toxicity; D000975:Antioxidants / Q000302:isolation & purification / Q000627:therapeutic use* / Q000633:toxicity; D002351:Carrageenan / Q000633:toxicity; D004353:Drug Evaluation, Preclinical; D004487:Edema / Q000139:chemically induced / Q000517:prevention & control; D004910:Erythrocyte Membrane / Q000187:drug effects; D000431:Ethanol; D006636:Histamine Release / Q000187:drug effects*; D006801:Humans; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy*; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D008407:Mast Cells / Q000187:drug effects; D008515:Medicine, Ayurvedic; D051379:Mice; D008517:Phytotherapy; D010936:Plant Extracts / Q000302:isolation & purification / Q000627:therapeutic use* / Q000633:toxicity; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats; D029846:Senna Plant / Q000737:chemistry*; D012997:Solvents; D014867:Water
D018926:Anti-Allergic Agents; D000894:Anti-Inflammatory Agents, Non-Steroidal; D000975:Antioxidants; D010936:Plant Extracts; D012997:Solvents; D014867:Water; D000431:Ethanol; D002351:Carrageenan