Design of a standalone-type beryllium vessel for high-pressure protein crystallography.
A standalone-type beryllium (Be) high-pressure crystallography vessel has been developed. Using a coupler-joint unit and a pressure valve, we could keep the pressure in the vessel constant at 100+/-1 MPa for more than 24 h without connecting to a pressure-generating apparatus. Diffraction spots of a glucose isomerase (GI) crystal under 100 MPa were collected using the vessel and a rotating copper-anode in-house x-ray generator (0.8 kW). We successfully collected a 2.0 A resolution data set of a 0.5 mm size GI crystal in an aqueous solution at 100 MPa.
Suzuki
Yoshihisa
Y
Department of Life System, Institute of Technology and Science, The University of Tokushima, 2-1 Minamijosanjima, Tokushima 770-8506, Japan. suzuki@chem.tokushima-u.ac.jp
Tsukamoto
Masayuki
M
Sakuraba
Haruhiko
H
Matsumoto
Masamitsu
M
Nagasawa
Makoto
M
Tamura
Katsuhiro
K
D001608:Beryllium; D003461:Crystallography / Q000295:instrumentation*; D018360:Crystallography, X-Ray; D004867:Equipment Design / Q000379:methods*; D011312:Pressure; D011506:Proteins / Q000737:chemistry*
D011506:Proteins; D001608:Beryllium