Surface plasmon resonance biosensor based on Fe3O4/Au nanocomposites.
In this paper, the core/shell Fe3O4/Au nanocomposites modified with 3-mercaptopropionic acid (MPA) were prepared and applied in the surface plasmon resonance (SPR) biosensor. And the detailed investigation of Fe3O4/Au nanocomposites was separately performed by UV-vis spectroscopy and transmission electronic microscopy. As the magnetic property and exceptional optical properties, the Fe3O4/Au nanocomposites were used as the solid support for the goat anti-human IgM, which could be immobilized on the surface of SPR biosensor chip by a magnetic pillar. This novel method of immobilizing goat anti-human IgM simplified experimental procedures and facilitated the regeneration of the sensing membrane. In addition, the different diameter of Fe3O4/Au nanocomposites could be obtained with the different amount of MPA in the solution. And the effect of Fe3O4/Au nanocomposites with different diameters on the sensitivity of SPR biosensor was also explored. As a result, the SPR biosensor exhibits a satisfactory response for human IgM in the concentration range of 0.30-20.00 μg ml(-1) and the increasing nanocomposite diameter is in favor of the sensitivity enhancement of SPR biosensor.
Wang
Jian
J
College of Chemistry, Jilin University, Qianjin Street No. 2699, Changchun 130012, PR China.
Sun
Ying
Y
Wang
Liying
L
Zhu
Xiaonan
X
Zhang
Hanqi
H
Song
Daqian
D
D015097:3-Mercaptopropionic Acid / Q000737:chemistry; D052203:Ferrosoferric Oxide / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D006801:Humans; D007075:Immunoglobulin M / Q000032:analysis*; D008280:Magnetics; D053761:Nanocomposites / Q000737:chemistry*; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D010316:Particle Size; D012680:Sensitivity and Specificity; D020349:Surface Plasmon Resonance / Q000379:methods*; D013499:Surface Properties
D007075:Immunoglobulin M; D009942:Organometallic Compounds; D006046:Gold; D015097:3-Mercaptopropionic Acid; D052203:Ferrosoferric Oxide