title: [Application of immunohistochemical labeling in the diagnosis of hepatobiliary intraepithelial neoplasia and/or cancer].
abstract: OBJECTIVE
  To investigate the best combination of monoclonal antibodies for the diagnosis of hepatobiliary intraepithelial neoplasia and/or cancer.
  
  
  METHODS
  CK7, CK20, Villin, CEA, P53 and Ki-67 antigens were detected in the tissues of high-level hepatobiliary intraepithelial neoplasia and cancer by immunohistochemistry and the results were analyzed statistically.
  
  
  RESULTS
  Villin was 100% positive in hepatobiliary intraepithelial neoplasia and/or cancer, while 100% negative in the adjacent normal bile duct epithelium. The expression rate of CEA was significantly lower in high-level hepatobiliary intraepithelial neoplasia tissues than in the cancer tissues (P<0.05). Ki-67 indexes were significantly lower in most of the high-level hepatobiliary intraepithelial neoplasia than in the cancer tissue (P<0.01). P53 indexes were also lower in high-level hepatobiliary intraepithelial neoplasia (P<0.01).
  
  
  CONCLUSIONS
  Detection of multiple antigens (CEA, Villin, Ki-67 and P53) provides specific clues to the diagnosis of high-grade hepatobiliary intraepithelial neoplasia and/or cancer.
authors.affiliation: Department of Pathology, Hunan Provincial People's Hospital, Changsha 410005, China. lychglx @163.com
authors.forename: Yan-chun
authors.initials: YC
authors.lastname: Li
authors.forename: Hong-mei
authors.initials: HM
authors.lastname: Yi
authors.forename: Ren-hua
authors.initials: RH
authors.lastname: Zhong
authors.forename: Zhuo-ri
authors.initials: ZR
authors.lastname: Li
mesh_terms: D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm / Q000032:analysis*; D001650:Bile Duct Neoplasms / Q000175:diagnosis* / Q000473:pathology; D001653:Bile Ducts, Intrahepatic / Q000473:pathology*; D002278:Carcinoma in Situ / Q000175:diagnosis* / Q000473:pathology; D004848:Epithelium / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged
chemical_list: D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm