[Protein kinase D3 is involved in negative regulation of MMP-7 in prostate cancer cells].
OBJECTIVE
To explore the role of protein kinase D3 (PKD3) in the regulation of matrix metalloproteinases 7 (MMP-7) expression in prostate cancer cells.


METHODS
PC-3 cells were either stimulated with 100 nmol/L PMA to activate PKD3 kinase activity, or transiently transfected with PKD3 siRNA, and the relative expression level of MMP-7 mRNA were analyzed by real-time PCR using 2(-delta delta Ct) method. MMP-7 mRNA levels were also analyzed and quantified in HEK293 cells with over-expression of wild-type PKD3, PKD3 knockdown (using PKD3 siRNA), or over-expression of wild-type PKD3 followed by PKD3 knockdown.


RESULTS
MMP-7 mRNA expression in PC3 cells was significantly decreased after PMA-induced PKD3 kinase activation. In contrast, PKD3 knockdown by siRNA transfection markedly increased MMP-7 mRNA level (P<0.01). MMP-7 mRNA level in HEK293 cells was significantly decreased by PKD3 over-expression, whereas obviously increased by PKD3 knockdown. Down-regulation of MMP-7 mRNA level in HEK293 induced by PKD3 over-expression was rescued by PKD3 knockdown.


CONCLUSIONS
PKD3 may contribute to the malignant progression of prostate cancer cells through negative regulation of MMP-7 expression.
Zou
Zhi-peng
ZP
Department of Cell Biology, Southern Medical University, Guangzhou 510515, China. zzp@fimmu.com
Feng
Li
L
Xu
Wan-fu
WF
Ke
Zhi-yong
ZY
Wang
Q Jane
QJ
Deng
Fan
F
D045744:Cell Line, Tumor; D015536:Down-Regulation; D055785:Gene Knockdown Techniques; D006801:Humans; D008297:Male; D020783:Matrix Metalloproteinase 7 / Q000378:metabolism*; D011471:Prostatic Neoplasms / Q000201:enzymology / Q000378:metabolism*; D011493:Protein Kinase C / Q000378:metabolism*; D015398:Signal Transduction
C119190:protein kinase C nu; D011493:Protein Kinase C; C060932:MMP7 protein, human; D020783:Matrix Metalloproteinase 7