Anticholinesterase and antioxidant constituents from Gloiopeltis furcata.
Activity-directed isolation of the ethyl acetate, methylene chloride and n-hexane fractions of Gloiopeltis furcata resulted in the isolation of 18 compounds. Their structures were elucidated as 2-(3-hydroxy-5-oxotetrahydrofuran-3-yl)acetic acid (1), glutaric acid (2), succinic acid (3), nicotinic acid (4), (E)-4-hydroxyhex-2-enoic acid (5), cholesterol (6), 7-hydroxycholesterol (7), uridine (8), glycerol (9), 5-(hydroxymethyl)-2-methoxybenzene-1,3-diol (10), (5E,7E)-9-oxodeca-5,7-dienoic acid (11), (Z)-3-ethylidene-4-methylpyrrolidine-2,5-dione (12), dehydrovomifoliol (13), loliolide (14), cholesteryl stearate (15), palmitic acid (16), cis-5,8,11,14,17-eicosapentaenoic acid (17) and alpha-linolenic acid (18) on the basis of spectroscopic and chemical evidences. Their anticholinesterase and antioxidant activities were evaluated via inhibitory activities on acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) as well as scavenging activities on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and peroxynitrite (ONOO(-)). All isolated compounds (1-18) exhibited moderate AChE inhibitory activities with IC(50) values ranging from 1.14-12.50 microg/ml, whereas 1, 7, 9, 17, and 18 showed mild BChE inhibitory activities with IC(50) values ranging from 5.57-15.89 microg/ml. Although most of the compounds isolated were lacking the scavenging activity on DPPH radical and ONOO(-), 5 and 10 showed good DPPH radical scavenging activity, and 5, 10, and 16 showed potent ONOO(-) scavenging activity.
Fang
Zhe
Z
College of Pharmacy, Catholic University of Daegu, Gyeongbuk 712-702, Republic of Korea.
Jeong
Su Yang
SY
Jung
Hyun Ah
HA
Choi
Jae Sue
JS
Min
Byung Sun
BS
Woo
Mi Hee
MH
D000110:Acetylcholinesterase / Q000378:metabolism; D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000378:metabolism; D002091:Butyrylcholinesterase / Q000378:metabolism; D002800:Cholinesterase Inhibitors / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D005609:Free Radicals / Q000378:metabolism; D015394:Molecular Structure; D010851:Picrates / Q000378:metabolism; D000461:Rhodophyta / Q000737:chemistry*; D013329:Structure-Activity Relationship
D000975:Antioxidants; D001713:Biphenyl Compounds; D002800:Cholinesterase Inhibitors; D005609:Free Radicals; D010851:Picrates; C004931:1,1-diphenyl-2-picrylhydrazyl; D000110:Acetylcholinesterase; D002091:Butyrylcholinesterase