Role of substance P (SP) and calcitonin gene-related peptide (CGRP) in gibbon-ape-leukemia virus (GALV) transduction of CD34+ cells.
OBJECTIVE
Optimization of transduction condition is an important goal to improve gene transduction. Therefore, we aimed to assess the effect of SP and/or CGRP as novel growth/transducing factors on the efficacy of CD34(+) transduction.


METHODS
CD34(+) cells were transduced with Gibbon-Ape-Leukemia virus (GALV), containing Neomycin gene. CD34(+) cells were transduced with GALV presence of SP and/or CGRP. Real Time PCR and colony formation assay (CFU-C) was performed.


RESULTS
Viral vectors titration on Hela cells indicated transduction efficiency of 1×10(6)CFU/ml. Real Time PCR of Neo and CFU-C showed stimulatory role of SP on gene transfer 5.9% and 14.84% compared to 3.6% and 12.58% in control group, while opposite role observed for CGRP 0.89% and 7.86%. Both SP and CGRP showed no significant effect in these assays.


CONCLUSIONS
This study showed including of SP in growth factor cocktail is beneficial for CD34(+) transduction, which could be applied to genetic modification procedures.
Shahrokhi
Somayeh
S
Department of Immunology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Alimoghaddam
Kamran
K
Ghavamzadeh
Ardeshir
A
D018952:Antigens, CD34 / Q000378:metabolism*; D001773:Blood Cells / Q000187:drug effects* / Q000502:physiology*; D015740:Calcitonin Gene-Related Peptide / Q000494:pharmacology*; D002478:Cells, Cultured; D005312:Fetal Blood / Q000166:cytology; D018014:Gene Transfer Techniques; D006367:HeLa Cells; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000494:pharmacology; D017989:Leukemia Virus, Gibbon Ape / Q000235:genetics*; D013373:Substance P / Q000494:pharmacology*; D014161:Transduction, Genetic
D018952:Antigens, CD34; D036341:Intercellular Signaling Peptides and Proteins; D013373:Substance P; D015740:Calcitonin Gene-Related Peptide