title: Molecular characterization of adenoviruses from children presenting with acute respiratory disease in Uberlândia, Minas Gerais, Brazil, and detection of an isolate genetically related to feline adenovirus.
abstract: Human adenoviruses (HAdV) are a major cause of acute respiratory diseases (ARD), gastroenteritis, conjunctivitis and urinary infections. Between November 2000-April 2007, a total of 468 nasopharyngeal aspirate samples were collected from children with ARD at the Clinics Hospital of Uberlândia. These samples were tested by immunofluorescence assay (IFA) and 3% (14/468) tested positive for the presence of HAdV. By performing polymerase chain reaction (PCR) to detect HAdV DNA in samples that tested negative or inconclusive for all viruses identifiable by IFA (respiratory syncytial virus, parainfluenza viruses 1, 2 and 3, influenza viruses A and B and HAdV), as well as negative for rhinoviruses by reverse transcription-PCR, additional 19 cases were detected, for a total of 33 (7.1%) HAdV-positive samples. Nucleotide sequences of 13 HAdV samples were analyzed, revealing that they belonged to species B, C and E. Further analyses showed that species C (HAdV-2) was the most prevalent among the sequenced samples. To our knowledge, this is the first report describing the presence of HAdV-4 in Brazil. We also detected an isolate that was 100% identical to a part of the feline adenovirus hexon gene sequence.
authors.affiliation: Laboratório de Virologia, Instituto de Ciências Biomédicas, Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, Uberlândia, MG, Brasil.
authors.forename: Lysa Nepomuceno
authors.initials: LN
authors.lastname: Luiz
authors.forename: José Paulo Gagliardi
authors.initials: JP
authors.lastname: Leite
authors.forename: Jonny
authors.initials: J
authors.lastname: Yokosawa
authors.forename: Bruno M
authors.initials: BM
authors.lastname: Carneiro
authors.forename: Edson
authors.initials: E
authors.lastname: Pereira Filho
authors.forename: Thelma Fátima de Mattos
authors.initials: TF
authors.lastname: Oliveira
authors.forename: Guilherme Ramos Oliveira e
authors.initials: GR
authors.lastname: Freitas
authors.forename: Lourenço Faria
authors.initials: LF
authors.lastname: Costa
authors.forename: Nayhanne Tizzo de
authors.initials: NT
authors.lastname: Paula
authors.forename: Hélio Lopes da
authors.initials: HL
authors.lastname: Silveira
authors.forename: Júlio César
authors.initials: JC
authors.lastname: Nepomuceno
authors.forename: Divina Aparecida Oliveira
authors.initials: DA
authors.lastname: Queiróz
mesh_terms: D000258:Adenovirus Infections, Human / Q000821:virology*; D000260:Adenoviruses, Human / Q000235:genetics* / Q000302:isolation & purification; D000818:Animals; D001483:Base Sequence; D001938:Brazil; D002415:Cats; D002675:Child, Preschool; D004279:DNA, Viral / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D009305:Nasopharynx / Q000821:virology*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012141:Respiratory Tract Infections / Q000175:diagnosis / Q000821:virology*; D012621:Seasons
chemical_list: D004279:DNA, Viral