A rapid immunochromatographic test strip for detecting rabies virus antibody.
An immunochromatographic test strip (ICTS) for detecting antibodies to rabies virus was developed, using colloidal gold particles labeled with rabies virus glycoprotein as the tracer. The assay was evaluated using sera from dogs immunized with various commercial rabies vaccines, or from dogs in the clinics and sera from dogs immunized with vaccines against pathogens other than rabies virus, and negative sera from a wide variety of animal sources, including dogs, mice, and cats which had never been vaccinated. The ICTS was found to be highly specific for antibodies against rabies virus, with a detection limit of 0.5IU/ml as measured by the fluorescent antibody virus neutralization (FAVN) test. Compared with the FAVN test, the specificity and sensitivity of ICTS were 98.2% and 90.4%, respectively. There was an excellent agreement between results obtained by the ICTS and FAVN tests (kappa=0.888). Strips stored at 4°C in a plastic bag with a desiccant retained their specificity and sensitivity for at least 15 months, and strips stored at ambient temperature remained stable for 12 months. The immunochromatographic test strip may therefore be useful for clinical laboratories lacking specialized equipment and for diagnosis in the field for rapid detection of rabies virus-specific antibodies.
Wang
Hualei
H
College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi An Road, Changchun, Jilin, China.
Feng
Na
N
Yang
Songtao
S
Wang
Chengyu
C
Wang
Tiecheng
T
Gao
Yuwei
Y
Su
Jianqing
J
Zheng
Xuexing
X
Hou
Xiaoqiang
X
Huang
Hainan
H
Yang
Ruimei
R
Zou
Xiaohuan
X
Huang
Geng
G
Xia
Xianzhu
X
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D015153:Blotting, Western; D002415:Cats; D004285:Dogs; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D006023:Glycoproteins / Q000276:immunology; D017979:Gold Colloid; D007118:Immunoassay / Q000379:methods*; D051379:Mice; D009500:Neutralization Tests; D011818:Rabies / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D011819:Rabies Vaccines / Q000276:immunology; D011820:Rabies virus / Q000276:immunology*; D011934:Reagent Strips; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014764:Viral Proteins / Q000276:immunology
D000914:Antibodies, Viral; D006023:Glycoproteins; D017979:Gold Colloid; D011819:Rabies Vaccines; D011934:Reagent Strips; D014764:Viral Proteins