Determination of glutation peroxidase and superoxide dismutase activities in canine seminal plasma and its relation with sperm quality and lipid peroxidation post thaw.
Lipid peroxidation (LPO) of dog spermatozoa was assessed in fresh semen and in samples of the same ejaculates after freezing and thawing. Particular attention was paid to individual differences in the susceptibility to LPO and its possible relationship with freezeability. Innate levels of LPO were low in fresh spermatozoa but increased after thawing in one of the dogs included in our study. The level of lipid peroxidation in fresh spermatozoa was not correlated with that of thawed spermatozoa. Negative correlations were detected between the activity in seminal plasma of GPx and sperm velocities post thaw (P < 0.01), however SOD activity was positively correlated with the percentage of linear motile sperm post thaw (P < 0.05).
Neagu
V R
VR
Veterinary Teaching Hospital, University of Extremadura, Cáceres, Spain.
García
B Macías
BM
Rodríguez
A Morillo
AM
Ferrusola
C Ortega
CO
Bolaños
J M Gallardo
JM
Fernández
L González
LG
Tapia
J A
JA
Peña
F J
FJ
D000818:Animals; D002470:Cell Survival; D015925:Cryopreservation / Q000662:veterinary*; D004285:Dogs; D005979:Glutathione Peroxidase / Q000378:metabolism*; D015227:Lipid Peroxidation; D008297:Male; D012661:Semen / Q000201:enzymology*; D013081:Sperm Motility; D013094:Spermatozoa / Q000201:enzymology / Q000502:physiology* / Q000648:ultrastructure; D013482:Superoxide Dismutase / Q000378:metabolism*
D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase