Myoglobin as a new fluorescence probe to sense H2S.
A new, fast, simple and cost-effective sensing device for monitoring H(2)S has been developed. Proof-of-principle results showing that a commercial and cheap Myoglobin (Mb) can be successfully used as a biological probe for a fluorescence biosensor for H(2)S detection are reported. The two different commercial labels Cy3 and Atto620 were selected for this study. A high selectivity for detecting H(2)S against other thiols was found. The applicability of the proposed sensing system was successfully explored not only in solution but also when applied in the form of a solid state device.
Strianese
Maria
M
Department of Chemistry, University of Salerno, Fisciano (Sa), Italy.
De Martino
Franco
F
Pellecchia
Claudio
C
Ruggiero
Giuseppe
G
D'Auria
Sabato
S
D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D002232:Carbocyanines / Q000737:chemistry; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry*; D006862:Hydrogen Sulfide / Q000032:analysis*; D055767:Immobilized Proteins / Q000737:chemistry; D009211:Myoglobin / Q000737:chemistry*; D013696:Temperature
D002232:Carbocyanines; D005456:Fluorescent Dyes; D055767:Immobilized Proteins; D009211:Myoglobin; C089187:cyanine dye 3; D006862:Hydrogen Sulfide