title: Stable, site-specific fluorescent tagging constructs optimized for burkholderia species.
abstract: Several vectors that facilitate stable fluorescent labeling of Burkholderia pseudomallei and Burkholderia thailandensis were constructed. These vectors combined the effectiveness of the mini-Tn7 site-specific transposition system with fluorescent proteins optimized for Burkholderia spp., enabling bacterial tracking during cellular infection.
authors.affiliation: Department of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa, Honolulu, HI 96822, USA.
authors.forename: Michael H
authors.initials: MH
authors.lastname: Norris
authors.forename: Yun
authors.initials: Y
authors.lastname: Kang
authors.forename: Bruce
authors.initials: B
authors.lastname: Wilcox
authors.forename: Tung T
authors.initials: TT
authors.lastname: Hoang
mesh_terms: D019117:Burkholderia / Q000235:genetics*; D003001:Cloning, Molecular / Q000379:methods; D004251:DNA Transposable Elements; D005453:Fluorescence; D005822:Genetic Vectors; D005827:Genetics, Microbial / Q000379:methods; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008967:Molecular Biology / Q000379:methods; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D004251:DNA Transposable Elements; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins