Brain tumor stem cells maintain overall phenotype and tumorigenicity after in vitro culturing in serum-free conditions.
Traditional in vitro culturing of tumor cells has been shown to induce changes so that cultures no longer represent the tumor of origin. Serum-free culturing conditions are used in a variety of cancers to propagate stem-like cells in vitro. Limited reports, however, exist on the effects of such propagation. We have compared cells from brain tumor biopsies cultivated under serum-free conditions at passages 2 and 10 to describe the effects of in vitro culturing. We were able to establish cell lines from 7 of 10 biopsies from patients with glioblastoma. The cell lines adapted to conditions and had 2.2 times increased population doubling rate at later passages. Karyotyping and comparative genomic hybridization analysis revealed that all examined cell lines had cytogenetic aberrations commonly found in glioblastomas, and there were only minor differences between tumor and early and late passages in the same culture. Whole-transcriptome analysis shows that tumors had interindividual differences. Changes in the overall expression patterns through passaging were modest, with a significant change in only 14 genes; the variation among cultures was, however, reduced through passages. The ability to differentiate differed among tumors but was maintained throughout passaging. The cells initiated tumors upon transplantation to immunodeficient mice with differing phenotypes, but a given cell culture maintained tumor phenotype after serial cultivation. The cultures established maintained individual characteristics specific to culture identity. Thus, each cell culture reflects an image of the tumor--or a personalized model--from which it was derived and remains representative after moderate expansion.
Vik-Mo
Einar Osland
EO
Institute for Surgical Research, Norwegian National Hospital, Oslo, Norway. e.o.vik-mo@medisin.uio.no
Sandberg
Cecilie
C
Olstorn
Havard
H
Varghese
Mercy
M
Brandal
Petter
P
Ramm-Pettersen
Jon
J
Murrell
Wayne
W
Langmoen
Iver Arne
IA
D000818:Animals; D001254:Astrocytoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism; D001932:Brain Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D018929:Cell Culture Techniques; D002869:Chromosome Aberrations; D055028:Comparative Genomic Hybridization; D016895:Culture Media, Serum-Free; D004273:DNA, Neoplasm / Q000235:genetics; D020869:Gene Expression Profiling; D006801:Humans; D007621:Karyotyping; D051379:Mice; D016513:Mice, SCID; D014411:Neoplastic Stem Cells / Q000473:pathology*; D020411:Oligonucleotide Array Sequence Analysis; D010641:Phenotype; D016133:Polymerase Chain Reaction; D012334:RNA, Neoplasm / Q000235:genetics; D015398:Signal Transduction; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured
D014408:Biomarkers, Tumor; D016895:Culture Media, Serum-Free; D004273:DNA, Neoplasm; D012334:RNA, Neoplasm