Increased susceptibility to liver damage from pneumoperitoneum in a murine model of biliary atresia.
OBJECTIVE
We hypothesized that livers with biliary atresia (BA) are more susceptible to the harmful effects of a high-pressure CO(2) pneumoperitoneum (PP) than healthy livers.


METHODS
A murine model of BA was used in this experiment. Mice were divided into 6 groups: (1) control Balb/c; (2) control Balb/c, CO(2)-PP; (3) control BA; (4) BA-sham; (5) BA, CO(2)-PP; and (6) BA, air-PP. Mice from groups 2, 5, and 6 underwent an 8-mm Hg-PP for 60 minutes. Liver samples were collected for histology, colorimetry, and flow cytometry analysis 18 to 24 hours after the procedure. Markers of apoptosis were investigated as indicators of acute cell damage.


RESULTS
We observed a statistically significant higher rate of apoptosis in livers with BA exposed to a prolonged CO(2)-PP or air-PP compared with control groups. There were no significant differences between groups 1 and 2, or between groups 5 and 6.


CONCLUSIONS
In this animal model, we have shown that livers with BA are more susceptible than healthy livers to injury by a prolonged PP. This injury was caused by both CO(2) and air-PP, implying that it is the direct result of pressure. These results may have implications for the success of minimally invasive Kasai procedures.
Laje
Pablo
P
Department of General Surgery, The Children's Hospital of Philadelphia, Philadelphia, PA, USA.
Clark
Fred H
FH
Friedman
Joshua R
JR
Flake
Alan W
AW
D000818:Animals; D001656:Biliary Atresia / Q000150:complications / Q000601:surgery*; D004195:Disease Models, Animal; D004198:Disease Susceptibility; D017093:Liver Failure / Q000209:etiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011028:Pneumoperitoneum, Artificial / Q000009:adverse effects*