Protein resistant oligo(ethylene glycol) terminated self-assembled monolayers of thiols on gold by vapor deposition in vacuum.
Protein resistant oligo(ethylene glycol) (OEG) terminated self-assembled monolayers (SAMs) of thiols on gold are commonly used for suppression of nonspecific protein adsorption in biology and biotechnology. The standard preparation for these SAMs is the solution method (SM) that involves immersion of the gold surface in an OEG solution. Here the authors present the preparation of 11-(mercaptoundecyl)-triethylene glycol [HS(CH(2))(11)(OCH(2)CH(2))(3)OH] SAMs on gold surface by vapor deposition (VD) in vacuum. They compare the properties of SAMs prepared by VD and SM using x-ray photoelectron spectroscopy (XPS), polarization modulation infrared reflection absorption spectroscopy, and surface plasmon resonance measurements. VD and SM SAMs exhibit similar packing density and show a similar resistance to the nonspecific adsorption of various proteins (bovine serum albumin, trypsin, and myoglobin) under physiological conditions. A very high sensitivity of the OEG SAMs to x-ray radiation is found, which allows tuning their protein resistance. These results show a new path to in situ engineering, analysis, and patterning of protein resistant OEG SAMs by high vacuum and ultrahigh vacuum techniques.
Kankate
Laxman
L
Department of Physics, Physics of Supramolecular Systems and Surfaces, University of Bielefeld, 33615 Bielefeld, Germany.
Werner
Udo
U
Turchanin
Andrey
A
Gölzhäuser
Armin
A
Grossmann
Helge
H
Tampé
Robert
R
D000327:Adsorption; D000818:Animals; D001709:Biotechnology / Q000379:methods; D002417:Cattle; D019855:Ethylene Glycol / Q000138:chemical synthesis / Q000378:metabolism*; D006046:Gold; D006801:Humans; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D013438:Sulfhydryl Compounds / Q000138:chemical synthesis / Q000378:metabolism*; D014618:Vacuum; D014835:Volatilization
D011506:Proteins; D013438:Sulfhydryl Compounds; D006046:Gold; D019855:Ethylene Glycol