Changes in antioxidant systems in soybean as affected by Sclerotinia sclerotiorum (Lib.) de Bary.
Changes in antioxidant systems in soybean [Glycine max (L.) Merr., Fabaceae] genotypes infected with Sclerotinia sclerotiorum were studied 12, 24, 48 and 72h after inoculation. Generation of superoxide and hydroxyl radicals was evaluated together with the production of malonyldialdehyde, main end product of lipid peroxidation. Several enzymatic and non-enzymatic parameters were monitored as well, such as the activity of antioxidant enzymes superoxide dismutase and pyrogallol and guaiacol peroxidases, reduced glutathione, soluble proteins and total carotenoids content. Results showed that genotypes expressed oxidative burst as well as different antioxidant systems in response to biotic stress caused by pathogen invasion. It has been confirmed that, although hypersensitive cell death is efficient against biotrophic pathogens, it does not protect soybean plants against infection by the necrotrophic pathogen such as S. sclerotiorum. Still, some genotypes showed distinctive and combined activity of several biochemical parameters which may point to further directions in exploring host-pathogen relations and lead to selection and production of new genotypes with higher levels of tolerance.
Malenčić
Djordje
D
Faculty of Agriculture, University of Novi Sad, Trg Dositeja Obradovića 8, 21000 Novi Sad, Serbia. malencic@polj.uns.ac.rs
Kiprovski
Biljana
B
Popović
Milan
M
Prvulović
Dejan
D
Miladinović
Jegor
J
Djordjević
Vuk
V
D000222:Adaptation, Physiological / Q000235:genetics / Q000502:physiology; D000975:Antioxidants / Q000378:metabolism*; D001203:Ascomycota; D016923:Cell Death; D005838:Genotype; D054884:Host-Pathogen Interactions / Q000235:genetics; D015227:Lipid Peroxidation; D008315:Malondialdehyde / Q000378:metabolism; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology*; D057865:Plant Immunity / Q000235:genetics / Q000502:physiology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D016897:Respiratory Burst / Q000235:genetics; D013025:Glycine max / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology; D013312:Stress, Physiological / Q000235:genetics
D000975:Antioxidants; D017382:Reactive Oxygen Species; D008315:Malondialdehyde