Rubricoccus marinus gen. nov., sp. nov., of the family 'Rhodothermaceae', isolated from seawater.
A coccoid- and amorphous-shaped, non-gliding, reddish bacterium, designated SG-29(T), was isolated from seawater in the western North Pacific Ocean near Japan. The strain was Gram-stain-negative, obligately aerobic, heterotrophic and catalase-positive. Nitrate was reduced to nitrogen and acid was produced from aesculin, turanose, 2-keto-gluconate and arabinose. Growth occurred with 1-5 % NaCl (optimum 2 % NaCl) and at 5-37 °C (optimum 20-30 °C). The G+C content of genomic DNA was 68.9 mol% (HPLC). The predominant isoprenoid quinone was MK-7 and the major cellular fatty acids (>10 %) were iso-C₁₇:₁ω9c, C₁₇:₁ω8c and iso-C₁₇:₀. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SG-29(T) belonged to the phylum Bacteroidetes. The most closely related strain was Rhodothermus obamensis JCM 9785(T), with which the isolate exhibited 86.1 % 16S rRNA gene sequence similarity. On the basis of phenotypic and phylogenetic features, strain SG-29(T) is classified in a novel genus and species within the family 'Rhodothermaceae', for which the name Rubricoccus marinus gen. nov., sp. nov. ( = NBRC 107124(T)  = KCTC 23197(T)) is proposed.
Park
Sanghwa
S
Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-Ku, Tokyo 113-0032, Japan.
Yoshizawa
Susumu
S
Atmosphere and Ocean Research Institute, The University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8564, Japan.
Kogure
Kazuhiro
K
Atmosphere and Ocean Research Institute, The University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8564, Japan.
Yokota
Akira
A
Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-Ku, Tokyo 113-0032, Japan.
D000332:Aerobiosis; D041963:Bacteroidetes / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D001482:Base Composition; D002244:Carbon / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D052836:Heterotrophic Processes; D007564:Japan; D008969:Molecular Sequence Data; D009584:Nitrogen / Q000378:metabolism; D010140:Pacific Ocean; D010802:Phylogeny; D010860:Pigments, Biological / Q000378:metabolism; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism; D013696:Temperature
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010860:Pigments, Biological; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride; D002244:Carbon; D002374:Catalase; D009584:Nitrogen