title: Construction of Neospora caninum stably expressing TgSAG1 and evaluation of its protective effects against Toxoplasma gondii infection in mice.
abstract: Toxoplasma gondii and Neospora caninum are closely related apicomplexan parasites. The surface antigen 1 of T. gondii (TgSAG1) is a major immunodominant antigen and, therefore, is considered to be a good candidate for the development of an effective recombinant vaccine against toxoplasmosis. In this study, N. caninum stably expressing the TgSAG1 gene (Nc/TgSAG1) was constructed using pyrimethamine-resistant DHFR-TS and GFP genes as double-selection markers. The expression level, molecular weight, and antigenic property of recombinant TgSAG1 expressed by the Nc/TgSAG1 were similar to those of the native TgSAG1. The mice immunized with Nc/TgSAG1 induced TgSAG1-specific Th1-dominant immune responses and protected the mice from a lethal challenge infection with T. gondii. These results indicate that N. caninum may provide a new tool for the production of a live recombinant vector vaccine against toxoplasmosis in animals. To our knowledge, this is the first report to evaluate the usefulness of N. caninum-based live vaccine.
authors.affiliation: National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan.
authors.forename: Guohong
authors.initials: G
authors.lastname: Zhang
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Huang
authors.forename: Damdinsuren
authors.initials: D
authors.lastname: Boldbaatar
authors.forename: Banzragch
authors.initials: B
authors.lastname: Battur
authors.forename: Badgar
authors.initials: B
authors.lastname: Battsetseg
authors.forename: Houshuang
authors.initials: H
authors.lastname: Zhang
authors.forename: Longzheng
authors.initials: L
authors.lastname: Yu
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Yuzi
authors.initials: Y
authors.lastname: Luo
authors.forename: Shinuo
authors.initials: S
authors.lastname: Cao
authors.forename: Youn-Kyong
authors.initials: YK
authors.lastname: Goo
authors.forename: Junya
authors.initials: J
authors.lastname: Yamagishi
authors.forename: Jinlin
authors.initials: J
authors.lastname: Zhou
authors.forename: Shoufa
authors.initials: S
authors.lastname: Zhang
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Suzuki
authors.forename: Ikuo
authors.initials: I
authors.lastname: Igarashi
authors.forename: Takeshi
authors.initials: T
authors.lastname: Mikami
authors.forename: Yoshifumi
authors.initials: Y
authors.lastname: Nishikawa
authors.forename: Xuenan
authors.initials: X
authors.lastname: Xuan
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D000953:Antigens, Protozoan / Q000235:genetics / Q000276:immunology*; D005260:Female; D056724:Immunity, Humoral; D016056:Immunodominant Epitopes / Q000235:genetics / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D018688:Neospora / Q000235:genetics*; D015800:Protozoan Proteins / Q000235:genetics / Q000276:immunology*; D016052:Protozoan Vaccines / Q000276:immunology*; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology; D014122:Toxoplasma / Q000276:immunology; D014124:Toxoplasmosis, Animal / Q000276:immunology / Q000517:prevention & control*; D014162:Transfection
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D016056:Immunodominant Epitopes; D015800:Protozoan Proteins; D016052:Protozoan Vaccines; D011994:Recombinant Proteins; C047168:SAG1 antigen, Toxoplasma