title: Overexpression and gender-specific differences of SRC-3 (SRC-3/AIB1) immunoreactivity in human non-small cell lung cancer: an in vivo study.
abstract: Steroid receptor coactivator-3 (SRC-3) has been reported to be overexpressed in the development and progression of many tumor types. SRC-3 has been detected in several lung cancer cell lines, but its expression and clinical significance in non-small cell lung cancer (NSCLC) remain unclear. In this study, 48 NSCLC tissues were collected and tissue microarrays were performed. The expression of SRC-3 was examined using nickel-intensified IHC. The results showed that of these 48 cases, 18 (37.5%) exhibited high levels of SRC-3 immunoreactivity, 23 (47.9%) exhibited moderate levels of SRC-3 immunoreactivity, and 7 (14.6%) were negative; thus, the total frequency of SRC-3 overexpression was 85.4% (41/48). This SRC-3 overexpression frequency was similar to the overexpression frequency observed for squamous cell carcinoma and adenocarcinoma (82.1% vs 90%) and for metastasis and non-metastasis patients (84.6% vs 85.7%). Data analysis demonstrated a significantly higher overexpression frequency in male patients compared with that in female patients (88.6% vs 76.9%). However, female patients tended to have higher expression levels of SRC-3, as measured by immunoreactivity, than male patients. These results demonstrate a high frequency of SRC-3 overexpression in NSCLC with a gender difference, suggesting that there is a specific role for SRC-3 in the pathogenesis of NSCLC.
authors.affiliation: Department of Thoracic Surgery, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.
authors.forename: Haidong
authors.initials: H
authors.lastname: Wang
authors.forename: Dongmei
authors.initials: D
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Wu
authors.forename: Jiqiang
authors.initials: J
authors.lastname: Zhang
authors.forename: Deyu
authors.initials: D
authors.lastname: Guo
authors.forename: Qingliang
authors.initials: Q
authors.lastname: Wang
authors.forename: Tao
authors.initials: T
authors.lastname: Jing
authors.forename: Chengping
authors.initials: C
authors.lastname: Xu
authors.forename: Xiuwu
authors.initials: X
authors.lastname: Bian
authors.forename: Kang
authors.initials: K
authors.lastname: Yang
mesh_terms: D000328:Adult; D000368:Aged; D002289:Carcinoma, Non-Small-Cell Lung / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D056921:Nuclear Receptor Coactivator 3 / Q000032:analysis* / Q000096:biosynthesis* / Q000378:metabolism; D012727:Sex Characteristics; D046888:Tissue Array Analysis
chemical_list: C538866:NCOA3 protein, human; D056921:Nuclear Receptor Coactivator 3