title: Immunohistochemical application of D2-40 as basal cell marker in evaluating atypical small acinar proliferation of initial routine prostatic needle biopsy materials.
abstract: D2-40 has been recently discovered as a lymphatic endothelial cell marker, and some investigators have found that D2-40 is also expressed in myoepithelial cells of salivary gland or breast. In this study, we evaluated D2-40 expression of basal cells and applied D2-40 immunohistochemistry in the combination of P504S, cytokeratin 5, and p63 for ten lesions with atypical small acinar proliferation (ASAP) in initial prostatic needle biopsy. As a result, D2-40 was expressed in basal cells, lymphatic endothelial cells, and some stromal fibroblasts of normal prostatic tissue. Among ten ASAP lesions, the final diagnosis of seven lesions was resolved by combination immunohistochemistry. D2-40 was comparable to cytokeratin 5 and p63 as a basal cell marker, and there were no lesions that failed to provide an accurate final diagnosis using only D2-40 immunohistochemistry without cytokeratin 5 or p63. However, we found some D2-40-positive stromal fibroblasts or D2-40-positive lumen-collapsed lymphatic vessels neighboring atypical glands. Pathologists should pay attention to avoid recognizing these cells as basal cells. In conclusion, the combination of immunohistochemistry of P504S, cytokeratin 5, p63, and D2-40 may contribute to the accurate diagnosis of ASAP in the initial prostatic needle biopsy.
authors.affiliation: Department of Diagnostic Pathology, Kochi Red Cross Hospital, 2-13-51 Shin-honmachi, Kochi 780-8562, Japan. kurochankochi@yahoo.co.jp
authors.forename: Naoto
authors.initials: N
authors.lastname: Kuroda
authors.forename: Kazunobu
authors.initials: K
authors.lastname: Katto
authors.forename: Masato
authors.initials: M
authors.lastname: Tamura
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Shiotsu
authors.forename: Shoichiro
authors.initials: S
authors.lastname: Nakamura
authors.forename: Yuji
authors.initials: Y
authors.lastname: Ohtsuki
authors.forename: Ondrej
authors.initials: O
authors.lastname: Hes
authors.forename: Michal
authors.initials: M
authors.lastname: Michal
authors.forename: Kaori
authors.initials: K
authors.lastname: Inoue
authors.forename: Masahiko
authors.initials: M
authors.lastname: Ohara
authors.forename: Keiko
authors.initials: K
authors.lastname: Mizuno
authors.forename: Gang-Hong
authors.initials: GH
authors.lastname: Lee
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000911:Antibodies, Monoclonal / Q000032:analysis*; D058846:Antibodies, Monoclonal, Murine-Derived; D014408:Biomarkers, Tumor / Q000032:analysis*; D001707:Biopsy, Needle; D018267:Carcinoma, Acinar Cell / Q000737:chemistry / Q000175:diagnosis* / Q000473:pathology; D042241:Early Diagnosis; D006801:Humans; D007150:Immunohistochemistry; D053555:Keratin-5 / Q000302:isolation & purification; D008297:Male; D008565:Membrane Proteins / Q000302:isolation & purification; D008875:Middle Aged; D011467:Prostate / Q000473:pathology; D011471:Prostatic Neoplasms / Q000737:chemistry / Q000175:diagnosis* / Q000473:pathology; D019998:Racemases and Epimerases / Q000302:isolation & purification; D012680:Sensitivity and Specificity
chemical_list: D000911:Antibodies, Monoclonal; D058846:Antibodies, Monoclonal, Murine-Derived; D014408:Biomarkers, Tumor; C081876:CKAP4 protein, human; D053555:Keratin-5; D008565:Membrane Proteins; C500987:monoclonal antibody D2-40; D019998:Racemases and Epimerases; C108352:alpha-methylacyl-CoA racemase