title: Chemokines and chemokine receptors coordinate the inflammatory immune response in human cutaneous leishmaniasis.
abstract: Cutaneous leishmaniasis (CL) includes different clinical manifestations displaying diverse intensities of dermal inflammatory infiltrate. Diffuse CL (DCL) cases are hyporesponsive, and lesions show very few lymphocytes and a predominance of macrophages. In contrast, localized CL (LCL) cases are responsive to leishmanial antigen, and lesions exhibit granulocytes and mononuclear cell infiltration in the early phases, changing to a pattern with numerous lymphocytes and macrophages later in the lesion. Therefore, different chemokines may affect the predominance of cell infiltration in distinct clinical manifestations. In lesions from LCL patients, we examined by flow cytometry the presence of different chemokines and their receptors in T cells, and we verified a higher expression of CXCR3 in the early stages of LCL (less than 30 days of infection) and a higher expression of CCR4 in the late stages of disease (more than 60 days of infection). We also observed a higher frequency of T cells producing IL-10 in the late stage of LCL. Using immunohistochemistry, we observed a higher expression of CCL7, CCL17 in lesions from late LCL, as well as CCR4 suggesting a preferential recruitment of regulatory T cells in the late LCL. Comparing lesions from LCL and DCL patients, we observed a higher frequency of CCL7 in DCL lesions. These results point out the importance of the chemokines, defining the different types of cells recruited to the site of the infection, which could be related to the outcome of infection as well as the clinical form observed.
authors.affiliation: Department of Biochemistry and Immunology, School of Medicine of Ribeirão Preto, University of São Paulo, Brazil.
authors.forename: Ana P
authors.initials: AP
authors.lastname: Campanelli
authors.forename: Claudia I
authors.initials: CI
authors.lastname: Brodskyn
authors.forename: Viviane
authors.initials: V
authors.lastname: Boaventura
authors.forename: Claire
authors.initials: C
authors.lastname: Silva
authors.forename: Ana M
authors.initials: AM
authors.lastname: Roselino
authors.forename: Jackson
authors.initials: J
authors.lastname: Costa
authors.forename: Ana Cristina
authors.initials: AC
authors.lastname: Saldanha
authors.forename: Luiz Antônio Rodrigues
authors.initials: LA
authors.lastname: de Freitas
authors.forename: Camila Indiani
authors.initials: CI
authors.lastname: de Oliveira
authors.forename: Manoel
authors.initials: M
authors.lastname: Barral-Netto
authors.forename: João S
authors.initials: JS
authors.lastname: Silva
authors.forename: Aldina
authors.initials: A
authors.lastname: Barral
mesh_terms: D000293:Adolescent; D000328:Adult; D018925:Chemokines / Q000276:immunology* / Q000378:metabolism; D002648:Child; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D007249:Inflammation / Q000276:immunology*; D016773:Leishmaniasis, Cutaneous / Q000276:immunology* / Q000473:pathology*; D016774:Leishmaniasis, Diffuse Cutaneous / Q000276:immunology / Q000473:pathology; D008297:Male; D008875:Middle Aged; D019707:Receptors, Chemokine / Q000276:immunology* / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D055815:Young Adult
chemical_list: D018925:Chemokines; D019707:Receptors, Chemokine