title: Vascular effects of a sulfated polysaccharide from the red marine alga Solieria filiformis.
abstract: Anticoagulant and antithrombotic properties of sulfated-polysaccharides (SP) from marine algae are extensively exploited. However, reports on the vascular effects of SP from red algae are rare in the literature. The polysaccharide from Solieria filiformis (Sf-SP) was isolated by ion exchange chromatography, analyzed by agarose gel electrophoresis and tested in male Wistar rats. The inflammation studies were performed using the paw-edema model and the relaxant activity in isolated aorta pre-contracted with phenylephrine. The anticoagulant effect was evaluated by the test of partial thromboplastin activation time. The SP (1 mg/kg) was not anti-inflammatory, but induced acute edema with maximal activity at 30 min (0.35 +/- 0.04 mL) compared to controls (0.05 +/- 0.03 mL). Cumulative addition of Sf-SP in phenylephrine-contracted tissues produced relaxation with maximal inhibition of 69% (IC50 29.3 +/- 9.0 microg/mL) at 300 microg/mL in comparison to controls (0.51 +/- 0.09 g). Sf-SP also extended human plasma coagulation time by 2.1 times. These substances could be used as important tools for the study of vascular alterations.
authors.affiliation: Instituto Superior de Ciências Biomédicas, Universidade Estadual do Ceará, Av. Paranjana 1700, 60.740-000, Fortaleza-CE-Brazil. anassreuy@gmail.com
authors.forename: Ana Maria S
authors.initials: AM
authors.lastname: Assreuy
authors.forename: Grazielle C
authors.initials: GC
authors.lastname: Pontes
authors.forename: Natália V F C
authors.initials: NV
authors.lastname: Rodrigues
authors.forename: Daniel M
authors.initials: DM
authors.lastname: Gomes
authors.forename: Paulo A
authors.initials: PA
authors.lastname: Xavier
authors.forename: Glacio S
authors.initials: GS
authors.lastname: Araujo
authors.forename: Alexandre H
authors.initials: AH
authors.lastname: Sampaio
authors.forename: Benildo S
authors.initials: BS
authors.lastname: Cavada
authors.forename: Maria G
authors.initials: MG
authors.lastname: Pereira
authors.forename: Wladimir R L
authors.initials: WR
authors.lastname: Farias
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D000925:Anticoagulants / Q000494:pharmacology*; D008297:Male; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D000461:Rhodophyta / Q000737:chemistry*; D013431:Sulfates / Q000494:pharmacology; D014664:Vasodilation / Q000187:drug effects
chemical_list: D000893:Anti-Inflammatory Agents; D000925:Anticoagulants; D011134:Polysaccharides; D013431:Sulfates