title: Measuring the stoichiometry of functional PspA complexes in living bacterial cells by single molecule photobleaching.
abstract: We report a general method based on wide-field fluorescence imaging of single molecule photobleaching and the Chung-Kennedy algorithm to measure the stoichiometry of functional protein complexes in living bacterial cells.
authors.affiliation: Division of Biology, Imperial College London, London, UK SW7 2AZ.
authors.forename: Tchern
authors.initials: T
authors.lastname: Lenn
authors.forename: Christos N
authors.initials: CN
authors.lastname: Gkekas
authors.forename: Laurent
authors.initials: L
authors.lastname: Bernard
authors.forename: Christoph
authors.initials: C
authors.lastname: Engl
authors.forename: Goran
authors.initials: G
authors.lastname: Jovanovic
authors.forename: Martin
authors.initials: M
authors.lastname: Buck
authors.forename: Liming
authors.initials: L
authors.lastname: Ying
mesh_terms: D000465:Algorithms; D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D004926:Escherichia coli / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry*; D006360:Heat-Shock Proteins / Q000737:chemistry* / Q000378:metabolism; D007089:Image Enhancement; D008856:Microscopy, Fluorescence; D038761:Photobleaching; D013237:Stereoisomerism
chemical_list: D001426:Bacterial Proteins; D005456:Fluorescent Dyes; D006360:Heat-Shock Proteins; C069816:phage shock protein, Bacteria