title: Cyclen-hybrid compound captures copper to protect INS-1 cells from islet amyloid polypeptide cytotoxicity by inhibiting and lysing effects.
abstract: Human islet amyloid polypeptide (hIAPP) deposit is the hallmark of type 2 diabetes pathology. Here, we report that apo-cyclen, attached to a specific hIAPP recognition motif (NYGAIL), captured copper ions and became proteolytically active. This cyclen-NYGAIL-copper complex was able to interfere with hIAPP aggregation and cleave hIAPP. These activities rescued INS-1 cells from hIAPP induced cytotoxicity.
authors.affiliation: Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology (Ministry of Education), Department of Chemistry, Tsinghua University, Beijing 100084, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Hu
authors.forename: Ye-Ping
authors.initials: YP
authors.lastname: Yu
authors.forename: Wei
authors.initials: W
authors.lastname: Cui
authors.forename: Chuan-Lin
authors.initials: CL
authors.lastname: Fang
authors.forename: Wei-Hui
authors.initials: WH
authors.lastname: Wu
authors.forename: Yu-Fen
authors.initials: YF
authors.lastname: Zhao
authors.forename: Yan-Mei
authors.initials: YM
authors.lastname: Li
mesh_terms: D002460:Cell Line; D003300:Copper / Q000737:chemistry*; D000080027:Cyclams; D006571:Heterocyclic Compounds / Q000737:chemistry*; D006801:Humans; D058228:Islet Amyloid Polypeptide / Q000633:toxicity*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D000080027:Cyclams; D006571:Heterocyclic Compounds; D058228:Islet Amyloid Polypeptide; D003300:Copper; C038072:cyclen