title: Structure of dimeric F1F0-ATP synthase.
abstract: The structure of the dimeric ATP synthase from yeast mitochondria was analyzed by transmission electron microscopy and single particle image analysis. In addition to the previously reported side views of the dimer, top view and intermediate projections served to resolve the arrangement of the rotary c(10) ring and the other stator subunits at the F(0)-F(0) dimeric interface. A three-dimensional reconstruction of the complex was calculated from a data set of 9960 molecular images at a resolution of 27 Å. The structural model of the dimeric ATP synthase shows the two monomers arranged at an angle of ∼45°, consistent with our earlier analysis of the ATP synthase from bovine heart mitochondria (Minauro-Sanmiguel, F., Wilkens, S., and Garcia, J. J. (2005) Proc. Natl. Acad. Sci. U.S.A. 102, 12356-12358). In the ATP synthase dimer, the two peripheral stalks are located near the F(1)-F(1) interface but are turned away from each other so that they are not in contact. Based on the three-dimensional reconstruction, a model of how dimeric ATP synthase assembles to form the higher order oligomeric structures that are required for mitochondrial cristae biogenesis is discussed.
authors.affiliation: Department of Biology, Chemistry Faculty, National Autonomous University of Mexico, Mexico City 04510, Mexico.
authors.forename: Sergio J
authors.initials: SJ
authors.lastname: Couoh-Cardel
authors.forename: Salvador
authors.initials: S
authors.lastname: Uribe-Carvajal
authors.forename: Stephan
authors.initials: S
authors.lastname: Wilkens
authors.forename: José J
authors.initials: JJ
authors.lastname: García-Trejo
mesh_terms: D018360:Crystallography, X-Ray; D019281:Dimerization; D008928:Mitochondria / Q000201:enzymology*; D025261:Mitochondrial Proton-Translocating ATPases / Q000737:chemistry* / Q000378:metabolism*; D008958:Models, Molecular; D011487:Protein Conformation; D012441:Saccharomyces cerevisiae / Q000201:enzymology*
chemical_list: C474451:F1F0-ATP synthase; D025261:Mitochondrial Proton-Translocating ATPases