Diagnosis of thalassemia on dried blood spot samples by high performance liquid chromatography.
High performance liquid chromatography (HPLC) on fresh lysates is the standard test for identification of thalassemia. Samples in the form of dried blood spot(s) (DBS) mailed to reference laboratories where HPLC is available could be an alternative. Hemoglobin (Hb) on DBS at day 1, 7, 15 and 30 were analyzed by HPLC and compared to those analyzed from fresh liquid whole blood at day 0. A 100% consistent interpretation of β-thalassemia (β-thal) trait and β-thal/Hb E disease between liquid whole blood and DBS was observed when analyzing Hb A(2) on DBS at a level of 2.7-9.9% in conjunction with a lower MCV (<80 fL) and MCH (<27 pg) and analyzing β-thal/Hb E by using Hb E and Hb F at a level of 30-48% and >10%, respectively. Therefore, our results show that detection of thalassemia carriers using DBS is possible and is the alternative of choice in a low resource setting.
Pornprasert
Sakorn
S
Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang-Mai University, Chiang-Mai, Thailand. sakornmi001@yahoo.com
Kasemrad
Chutharat
C
Sukunthamala
Kanyakan
K
D001800:Blood Specimen Collection / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005319:Fetal Hemoglobin / Q000032:analysis; D005838:Genotype; D006443:Hemoglobin A2 / Q000032:analysis; D006446:Hemoglobin E / Q000032:analysis / Q000235:genetics; D006454:Hemoglobins / Q000032:analysis* / Q000235:genetics; D006461:Hemolysis; D006801:Humans; D015203:Reproducibility of Results; D013789:Thalassemia / Q000097:blood* / Q000175:diagnosis*; D013997:Time Factors
D006454:Hemoglobins; D006443:Hemoglobin A2; D006446:Hemoglobin E; D005319:Fetal Hemoglobin