Regulator of G-protein signaling 14 protein modulates Ca²+ influx through Cav1 channels.
Calcium flux through L-type voltage-activated calcium (Cav1) channels is crucial for regulating brain functions including memory formation and behavior. Alterations in Ca²+ homeostasis have been linked to many cognitive disorders, and understanding the regulation of this process is crucial for their remedy. Therefore, here, we have evaluated the effect of a multifunctional protein known to be involved in memory functions called regulator of G-protein signaling 14 (RGS-14) on Cav1 channel activity in neuronal cell lines NG108-15 and SH-SY5Y. RGS-14 protein produced significant reduction in Ca²+ influx in both cell lines and this effect was dependent on nifedipine-sensitive Cav1 channels. Thus, our results provide evidence supporting the idea that RGS-14 may facilitate the cognitive processing by modulating Cav1 channel-mediated intracellular Ca²+ transients.
Martín-Montañez
Elisa
E
Laboratory of Neurobiology at CIMES, University of Malaga, Malaga, Spain.
Acevedo
Maria José
MJ
López-Téllez
Juan Félix
JF
Duncan
Raymond Scott
RS
Mateos
Antonio González
AG
Pavía
José
J
Koulen
Peter
P
Khan
Zafar U
ZU
D002118:Calcium / Q000037:antagonists & inhibitors / Q000378:metabolism*; D020746:Calcium Channels, L-Type / Q000502:physiology*; D020013:Calcium Signaling / Q000502:physiology*; D051242:Caveolin 1 / Q000378:metabolism*; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D003071:Cognition / Q000502:physiology; D006801:Humans; D009474:Neurons / Q000378:metabolism* / Q000502:physiology; D020710:RGS Proteins / Q000235:genetics / Q000502:physiology*
C495540:CAV1 protein, human; D020746:Calcium Channels, L-Type; D051242:Caveolin 1; D020710:RGS Proteins; C491400:RGS14 protein, human; D002118:Calcium