title: DD genotype of ace gene I/D polymorphism is associated in a Turkish study population with osteoarthritis.
abstract: This study was conducted in Turkish osteoarthritis patients to determine the frequency of I/D polymorphism genotypes of angiotensin converting enzyme gene, and to examine the role of this polymorphism in osteoarthritis development. Genomic DNA obtained from 200 persons (135 patients with osteoarthritis and 65 healthy controls) was used in the study. DNA was multiplied by polymerase chain reaction using I and D allele-specific primers. Polymerase chain reaction products were assessed with CCD camera by being exposed to 2% agarose gel electrophoresis. There was statistically significant difference between the groups with respect to genotype distribution (P < 0.001). The D allele frequency was indicated as 69% and I allele was as 31% in the patients, whereas it was 55-45% in the control group. Consequently, in this study, we may assert that ACE gene I/D polymorphism DD genotype determination is significant criteria for identifying patients who are likely to develop osteoarthritis in east population of Turkey.
authors.affiliation: Department of Biology, Faculty of Arts and Science, Muş Alparslan University, İstasyon Street, 49100 Muş, Turkey. bayrambanu@hotmail.com
authors.forename: Banu
authors.initials: B
authors.lastname: Bayram
authors.forename: Emrah
authors.initials: E
authors.lastname: Sayın
authors.forename: Hasan Veysi
authors.initials: HV
authors.lastname: Güneş
authors.forename: Irfan
authors.initials: I
authors.lastname: Değirmenci
authors.forename: Züleyha
authors.initials: Z
authors.lastname: Türkoğlu
authors.forename: Fulya
authors.initials: F
authors.lastname: Doganer
authors.forename: Didem Turgut
authors.initials: DT
authors.lastname: Coşan
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006801:Humans; D054643:INDEL Mutation / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D010003:Osteoarthritis / Q000201:enzymology* / Q000235:genetics*; D007703:Peptidyl-Dipeptidase A / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D014421:Turkey
chemical_list: C488034:ACE protein, human; D007703:Peptidyl-Dipeptidase A