Cell free expression put on the spot: advances in repeatable protein arraying from DNA (DAPA).
We have previously described the 'DNA array to protein array' (DAPA) method for microarraying of proteins expressed by cell-free systems in situ on the array surface. In this technique, a DNA array on one slide acts as the template for generating a protein array on a second slide, mediated by a cell free lysate between the two juxtaposed slides. Here we explore the feature of the repeatability of the technology, in which the same DNA array is reused several times, and use the method to generate a microarray of 116 diverse proteins. The capabilities of DAPA technology in comparison with other protein array methods are discussed.
Stoevesandt
Oda
O
Protein Technology Group, Babraham Bioscience Technologies, Cambridge CB22 3AT, UK. oda.stoevesandt@bbsrc.ac.uk
Vetter
Maike
M
Kastelic
Damjana
D
Palmer
Elizabeth A
EA
He
Mingyue
M
Taussig
Michael J
MJ
D000595:Amino Acid Sequence; D001483:Base Sequence; D002474:Cell-Free System; D004247:DNA / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D040081:Protein Array Analysis / Q000379:methods*; D011506:Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
D011506:Proteins; D004247:DNA