title: Identification of novel Cryptosporidium species in aquarium fish.
abstract: Little is known about the prevalence and genotypes of Cryptosporidium in fish. The present study investigated the prevalence of Cryptosporidium species in 200 aquarium fish of 39 different species in Western Australia by PCR amplification at the 18S rRNA locus. A total of 21 positives were detected by PCR (10.5% prevalence) from 13 different species of fish. Nineteen of these isolates were successfully sequenced. Of these, 12 were similar or identical to previously described species/genotypes of Cryptosporidium, while the remaining seven isolates appeared to represent three novel species.
authors.affiliation: Department of Fisheries, Faculty of Marine Natural Resources, Khoramshahr Marine Science and Technology, Khoramshahr, Khouzestan, Iran.
authors.forename: N
authors.initials: N
authors.lastname: Zanguee
authors.forename: J A
authors.initials: JA
authors.lastname: Lymbery
authors.forename: J
authors.initials: J
authors.lastname: Lau
authors.forename: A
authors.initials: A
authors.lastname: Suzuki
authors.forename: R
authors.initials: R
authors.lastname: Yang
authors.forename: J
authors.initials: J
authors.lastname: Ng
authors.forename: U
authors.initials: U
authors.lastname: Ryan
mesh_terms: D000818:Animals; D003457:Cryptosporidiosis / Q000453:epidemiology / Q000662:veterinary*; D003458:Cryptosporidium / Q000145:classification* / Q000235:genetics; D005393:Fish Diseases / Q000469:parasitology*; D005399:Fishes; D005838:Genotype; D008969:Molecular Sequence Data; D010802:Phylogeny; D015995:Prevalence; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D014904:Western Australia
chemical_list: D012337:RNA, Ribosomal, 18S