Encapsulation and regeneration of in vitro derived Zephyranthes grandiflora: an effective way for exchange of germplasm.
An attempt was made to develop a protocol for the preparation of synthetic seeds using in vitro regenerated, genetically identical bulbs of Zephyranthes grandiflora. Encapsulation was standardized with 4% sodium alginate and 1% sucrose for uniform bulb size with high conversion potential. An optimum storage temperature was found to be 4 degrees C. Synthetic seeds were germinated in MS medium supplemented with benzyladenine (2 mg dm(-3)) and rooted in the presence of indole-3-butyric acid (1 mg dm(-3)) in MS medium. Well-rooted plants were transferred to the experimental field with 80% survival after hardening. This study elucidated an efficient technique for exchange of germplasm and ex situ conservation method.
Gangopadhyay
Moumita
M
Medicinal Plant Laboratory, Department of Botany, Bose Institute, 93/1 APC Road, Kolkata 700009, India. moumita_gangopadhyay@yahoo.co.uk
Dewanjee
Saikat
S
Chakraborty
Dipjyoti
D
Bhattacharya
Sabita
S
D003470:Culture Media; D018525:Germination; D019668:Liliaceae / Q000254:growth & development*; D012038:Regeneration; D012639:Seeds / Q000502:physiology
D003470:Culture Media