Protein quantitation using mass spectrometry.
Mass spectrometry is a method of choice for quantifying low-abundance proteins and peptides in many biological studies. Here, we describe a range of computational aspects of protein and peptide quantitation, including methods for finding and integrating mass spectrometric peptide peaks, and detecting interference to obtain a robust measure of the amount of proteins present in samples.
Zhang
Guoan
G
Kimmel Center for Biology and Medicine at the Skirball Institute and Department of Pharmacology, New York University School of Medicine, New York, NY, USA.
Ueberheide
Beatrix M
BM
Waldemarson
Sofia
S
Myung
Sunnie
S
Molloy
Kelly
K
Eriksson
Jan
J
Chait
Brian T
BT
Neubert
Thomas A
TA
Fenyö
David
D
D000595:Amino Acid Sequence; D007554:Isotopes; D013058:Mass Spectrometry / Q000379:methods*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010455:Peptides / Q000032:analysis / Q000737:chemistry; D011506:Proteins / Q000032:analysis* / Q000737:chemistry
D007554:Isotopes; D010455:Peptides; D011506:Proteins