Preparation of styrene-co-4-vinylpyridine magnetic polymer beads by microwave irradiation for analysis of trace 24-epibrassinolide in plant samples using high performance liquid chromatography.
In the study, a kind of novel styrene-co-4-vinylpyridine (St-co-4-VP) porous magnetic polymer beads was prepared by microwave irradiation using suspension polymerization. Microwave heating preparation greatly reduced the polymerization time to 1h. Physical characteristic tests suggested that these beads were cross-linking and possessed spherical shape, good magnetic response and porous morphologies with a narrow diameter distribution of 70-180 μm. Therefore, these beads displayed the long-term stability after undergoing 100-time extractions. Then, an analytical method for the determination of trace 24-epiBR in plant samples was developed by magnetic polymer bead extraction coupled with high performance liquid chromatography-fluorescence detection. St-co-4-VP magnetic polymer beads demonstrated the higher extraction selectivity for 24-epiBR than other reference compounds. Linear range was 10.00-100.0 μg/L with a relative standard deviation (RSD) of 6.7%, and the detection limit was 6.5 μg/kg. This analytical method was successfully applied to analyze the trace 24-epiBR in cole and breaking-wall rape pollen samples with recoveries of 77.2-90.0% and 72.3-83.4%, respectively, and RSDs were less than 4.1%. The amount of 24-epiBR in real breaking-wall rape pollen samples was found to be 26.2 μg/kg finally. This work proposed a sensitive, rapid, reliable and convenient analytical method for the determination of trace brassinosteroids in complicated plant samples by the use of St-co-4-VP magnetic polymer bead extraction coupled with chromatographic method.
Zhang
Zhuomin
Z
School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, China.
Zhang
Yi
Y
Tan
Wei
W
Li
Gongke
G
Hu
Yuling
Y
D060406:Brassinosteroids; D002777:Cholestanols / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004574:Electromagnetic Fields; D016014:Linear Models; D008863:Microspheres; D008872:Microwaves; D010944:Plants / Q000737:chemistry; D011058:Pollen / Q000737:chemistry*; D058105:Polymerization; D011137:Polystyrenes / Q000737:chemistry*; D011145:Polyvinyls / Q000737:chemistry*; D011725:Pyridines / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013260:Steroids, Heterocyclic / Q000032:analysis*
D060406:Brassinosteroids; D002777:Cholestanols; D011137:Polystyrenes; D011145:Polyvinyls; D011725:Pyridines; D013260:Steroids, Heterocyclic; C509607:polystyrene-block-poly(4-vinylpyridine); C023623:brassinolide