Visualization of gold and platinum nanoparticles interacting with Salmonella enteritidis and Listeria monocytogenes.
OBJECTIVE
Rapid development of nanotechnology has recently brought significant attention to the extraordinary biological features of nanomaterials. The objective of the present investigation was to evaluate morphological characteristics of the assembles of gold and platinum nanoparticles (nano-Au and nano-Pt respectively), with Salmonella Enteritidis (Gram-negative) and Listeria monocytogenes (Gram-positive), to reveal possibilities of constructing bacteria-nanoparticle vehicles.


METHODS
Hydrocolloids of nano-Au or nano-Pt were added to two bacteria suspensions in the following order: nano-Au + Salmonella Enteritidis; nano-Au + Listeria monocytogenes; nano-Pt + Salmonella Enteritidis; nano-Pt + Listeria monocytogenes. Samples were inspected by transmission electron microscope.


RESULTS
Visualization of morphological interaction between nano-Au and Salmonella Enteritidis and Listeria monocytogenes, showed that nano-Au were aggregated within flagella or biofilm network and did not penetrate the bacterial cell. The analysis of morphological effects of interaction of nano-Pt with bacteria revealed that nano-Pt entered cells of Listeria monocytogenes and were removed from the cells. In the case of Salmonella Enteritidis, nano-Pt were seen inside bacteria cells, probably bound to DNA and partly left bacterial cells. After washing and centrifugation, some of the nano-Pt-DNA complexes were observed within Salmonella Enteritidis.


CONCLUSIONS
The results indicate that the bacteria could be used as a vehicle to deliver nano-Pt to specific points in the body.
Sawosz
Ewa
E
Division of Biotechnology and Biochemistry of Nutrition, Warsaw University of Life Sciences, Warsaw, Poland.
Chwalibog
André
A
Szeliga
Jacek
J
Sawosz
Filip
F
Grodzik
Marta
M
Rupiewicz
Marlena
M
Niemiec
Tomasz
T
Kacprzyk
Katarzyna
K
D001422:Bacterial Adhesion; D004269:DNA, Bacterial / Q000378:metabolism; D016503:Drug Delivery Systems / Q000379:methods*; D006046:Gold / Q000008:administration & dosage; D008089:Listeria monocytogenes / Q000502:physiology / Q000648:ultrastructure; D053768:Metal Nanoparticles / Q000008:administration & dosage* / Q000382:microbiology* / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D050997:Nanomedicine; D010984:Platinum / Q000008:administration & dosage; D012477:Salmonella enteritidis / Q000502:physiology / Q000648:ultrastructure
D004269:DNA, Bacterial; D010984:Platinum; D006046:Gold