[Synergistic interaction between MMP-3, VDR gene polymorphisms and occupational risk factors on lumbar disc degeneration].
OBJECTIVE
To explore the synergistic interaction between MMP-3,VDR gene polymorphisms and occupational risk factors on lumbar disc degeneration.


METHODS
A case-control study including 178 cases of lumbar disc degeneration and 284 controls was carried out through questionnaire and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technology. Additive model was used to analyze the synergistic interaction between gene polymorphisms and occupational risk factors.


RESULTS
The non-conditional logistic regression analysis showed that bending/twisting, whole-body vibration, heavy physical workload, alleles 5A of MMP-3 (6A5A/5A5A) and A of VDR-Apa (AA/Aa) were significantly associated with lumbar disc degeneration(OR = 4.06, 8.96, 5.46, 1.96 and 1.70, respectively, P < 0.05). There were synergistic interactions between the mutation genotype 5A of MMP-3 and whole-body vibration exposure, between the mutation genotype 5A of MMP-3 and bending/twisting, and between the mutation genotype A of VDR-Apa and whole-body vibration exposure (SI: 13.27, 2.91 and 2.35 respectively, P < 0.05).


CONCLUSIONS
People with the mutation genotypes 5A of MMP-3 and/or A of VDR-Apa may have the increased risk of developing lumbar disc degeneration if they are exposed to whole-body vibration and/or bending/twisting.
Yuan
Han-Yan
HY
Department of Occupational Health, School of Public Health, Fudan University, Shanghai 200032, China.
Tang
Ying
Y
Lei
Ling
L
Xiao
Guo-Bing
GB
Liang
You-Xin
YX
Wang
Sheng
S
Xia
Zhao-Lin
ZL
D000328:Adult; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D055959:Intervertebral Disc Degeneration / Q000235:genetics* / Q000473:pathology*; D008297:Male; D019278:Matrix Metalloproteinase 3 / Q000235:genetics*; D008875:Middle Aged; D016273:Occupational Exposure; D020641:Polymorphism, Single Nucleotide; D018167:Receptors, Calcitriol / Q000235:genetics*; D012307:Risk Factors
D018167:Receptors, Calcitriol; C511768:MMP3 protein, human; D019278:Matrix Metalloproteinase 3