title: Detection of metallo-β-lactamase-encoding genes among clinical isolates of Pseudomonas aeruginosa in northwest of Iran.
abstract: The prevalence of metallo-β-lactamase (MBL) production among 104 clinical isolates of Pseudomonas aeruginosa from northwest of Iran was investigated by phenotypic and polymerase chain reaction (PCR) methods. Thirty-nine (37.50%) of isolates were MBL positive by double-disk synergy test. Results of PCR revealed that 18 (17.31%) and 6 (5.77%) imipenem nonsusceptible isolates of P. aeruginosa carried bla(VIM) and bla(IMP) genes respectively, while 92.4% (62/67) of isolates contained class 1 integron gene. This is the first report of MBL-producing P. aeruginosa from northwest of Iran.
authors.affiliation: Biotechnology Research Center, Tabriz University of Medical Science, Tabriz, Iran.
authors.forename: Saber
authors.initials: S
authors.lastname: Yousefi
authors.forename: Safar
authors.initials: S
authors.lastname: Farajnia
authors.forename: Mohammd Reza
authors.initials: MR
authors.lastname: Nahaei
authors.forename: Mohammad Taghi
authors.initials: MT
authors.lastname: Akhi
authors.forename: Reza
authors.initials: R
authors.lastname: Ghotaslou
authors.forename: Mohammad Hossein
authors.initials: MH
authors.lastname: Soroush
authors.forename: Behrooz
authors.initials: B
authors.lastname: Naghili
authors.forename: Nima Hosseini
authors.initials: NH
authors.lastname: Jazani
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D004269:DNA, Bacterial / Q000235:genetics; D006801:Humans; D032023:Integrons; D007492:Iran; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction; D011552:Pseudomonas Infections / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000235:genetics*; D047090:beta-Lactams / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D047090:beta-Lactams; D001618:beta-Lactamases