[The role of protein kinase C in the process of HL-60 cells induced into dendritic cells by calcium ionophore A23187].
OBJECTIVE
To Investigate the role of protein kinase C (PKC) signal transduction in the process of HL-60 leukemic cells induced into dendritic cells (DCs) by calcium ionophore A23187.


METHODS
HL-60 cells were pretreated with protein kinase C (PKC) inhibitor Bis-1 for 24 hours followed by cultured with A23187 for another 36 hours, and the morphology, immunophenotype and function of stimulating proliferation of allogeneic T cells were compared with the cells only treated with A23187.


RESULTS
The morphological changes, surface marker expressions and ability to stimulating the proliferation of allogeneic T cells were inhibited in DCs derived from HL-60 cells treated with PKC inhibitor Bis-1. The percentage of CD83, CD80, CD86 expressing on DCs induced by A23187 significantly decreased to (28.97 +/- 4.05)% vs. (13.23 +/- 2.15)%, (19.10 +/- 5.46)% vs. (9.70 +/- 1.69)%, (41.03 +/- 6.43)% vs. (23.37 +/- 7.50)%, respectively (P < 0.05).


CONCLUSIONS
The induction of HL-60 cells induced into DCs by A23187 can be inhibited by PKC inhibitor Bis-1, this suggested that PKC signal transduction pathway might be involved in the process of HL-60 leukemic cells differentiated into DCs by A23187 induction.
Yang
Kun
K
Department of Pediatrics, Teaching Hospital of Chengdu University of TCM, Chengdu 610075, China.
Li
Qiang
Q
D000001:Calcimycin / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects*; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D003713:Dendritic Cells / Q000166:cytology*; D018922:HL-60 Cells; D006801:Humans; D007476:Ionophores / Q000494:pharmacology; D011493:Protein Kinase C / Q000378:metabolism* / Q000502:physiology
D007476:Ionophores; D000001:Calcimycin; D011493:Protein Kinase C; D002118:Calcium