title: Effect of cryopreservation on acetylation patterns of lysine 12 of histone H4 (acH4K12) in mouse oocytes and zygotes.
abstract: OBJECTIVE
  to determine the effect of cryopreservation on acH4K12 in oocytes and their respective zygotes.
  
  
  METHODS
  AcH4K12 in fresh or vitrified-warmed oocytes and their respective zygotes at 70 min-12 h post-fertilization were assessed using fluorescent staining.
  
  
  RESULTS
  1. AcH4K12 levels increased significantly in vitrified oocytes compared to controls. 2. Respective zygotes derived from vitrified oocytes had abnormal chromatin distribution or acH4K12 patterns before and after pronuclear formation.
  
  
  CONCLUSIONS
  Cryopreservation alters AcH4K12 patterns in oocytes, which subsequently affect the chromatin distribution and acH4K12 in fertilized oocytes.
authors.affiliation: College of Animal Science and State Key Laboratories for Agrobiotechnology, China Agricultural University, Beijing 100193, People's Republic of China.
authors.forename: Lun
authors.initials: L
authors.lastname: Suo
authors.forename: Qinggang
authors.initials: Q
authors.lastname: Meng
authors.forename: Yan
authors.initials: Y
authors.lastname: Pei
authors.forename: Xiangwei
authors.initials: X
authors.lastname: Fu
authors.forename: Yanping
authors.initials: Y
authors.lastname: Wang
authors.forename: Thomas D
authors.initials: TD
authors.lastname: Bunch
authors.forename: Shien
authors.initials: S
authors.lastname: Zhu
mesh_terms: D000107:Acetylation; D000818:Animals; D002843:Chromatin / Q000378:metabolism; D015925:Cryopreservation; D005260:Female; D006657:Histones / Q000378:metabolism*; D008239:Lysine / Q000378:metabolism; D051379:Mice; D009865:Oocytes / Q000166:cytology / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D015053:Zygote / Q000166:cytology / Q000378:metabolism*
chemical_list: D002843:Chromatin; D006657:Histones; D008239:Lysine