Leifsonia psychrotolerans sp. nov., a psychrotolerant species of the family Microbacteriaceae from Livingston Island, Antarctica.
A cold-tolerant, yellow-pigmented, Gram-positive, motile, facultatively anaerobic bacterial strain, LI1(T), was isolated from a moss-covered soil from Livingston Island, Antarctica, near the Bulgarian station St. Kliment Ohridski. Comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a clade with the species Leifsonia kafniensis KFC-22(T), Leifsonia pindariensis PON10(T) and Leifsonia antarctica SPC-20(T), with which it showed sequence similarities of 99.0, 97.9 and 97.9 %, respectively. DNA-DNA hybridization revealed a reassociation value of 2.7 % with L. kafniensis LMG 24362(T). The DNA G+C content of strain LI1(T) was 64.5 mol%. The growth temperature range was -6 to 28 °C, with optimum growth at 16 °C. Growth occurred in 0-5 % NaCl and at pH 4.5-9.5, with optimum growth in 1-2 % NaCl and at pH 5.5-6.5. The predominant fatty acids were anteiso-C(15 : 0), C(18 : 0) and iso-C(15 : 0). The polar lipids were phosphatidylglycerol and diphosphatidylglycerol. Physiological and biochemical tests clearly differentiated strain LI1(T) from L. kafniensis. Therefore, a novel cold-tolerant species within the genus Leifsonia is proposed: Leifsonia psychrotolerans sp. nov. (type strain LI1(T) = DSM 22824(T) = NCCB 100313(T)).
Ganzert
Lars
L
Alfred Wegener Institute for Polar and Marine Research, Research Department Potsdam, Telegrafenberg A45, 14473 Potsdam, Germany.
Bajerski
Felizitas
F
Alfred Wegener Institute for Polar and Marine Research, Research Department Potsdam, Telegrafenberg A45, 14473 Potsdam, Germany.
Mangelsdorf
Kai
K
Helmholtz Centre Potsdam GFZ German Research Centre for Geosciences, Section 4.3, Organic Geochemistry, Telegrafenberg B 423, 14473 Potsdam, Germany.
Lipski
André
A
Rheinische Friedrich-Wilhelms-Universität Bonn, Institute of Nutrition and Food Sciences, Department of Food Microbiology and Hygiene, Meckenheimer Allee 168, 53115 Bonn, Germany.
Wagner
Dirk
D
Alfred Wegener Institute for Polar and Marine Research, Research Department Potsdam, Telegrafenberg A45, 14473 Potsdam, Germany.
D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D000864:Antarctic Regions; D003080:Cold Temperature; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005227:Fatty Acids / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S