Praeruptorin A inhibits lipopolysaccharide-induced inflammatory response in murine macrophages through inhibition of NF-κB pathway activation.
Praeruptorin A (PA) is a pyranocoumarin compound isolated from the dried root of Peucedanum praeruptorum Dunn (Umbelliferae). However, the antiinflammatory effect of PA has not been reported. The present study investigated the antiinflammatory effect of PA in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophage cells. PA significantly inhibited the LPS-induced production of nitric oxide (NO), interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α). The mRNA and protein expressions of inducible nitric oxide synthase (iNOS), IL-1β and TNF-α were also suppressed by this compound. Further study showed that PA decreased the cytoplasmic loss of inhibitor κB-α (IκB-α) protein and inhibited the translocation of NF-κB from cytoplasm to nucleus. Taken together, the results suggest that PA may exert antiinflammatory effects in vitro in LPS-stimulated RAW 264.7 macrophages through inhibition of NF-κB signal pathway activation.
Yu
Peng-Jiu
PJ
School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, China.
Ci
Wei
W
Wang
Guang-Fa
GF
Zhang
Jun-Yan
JY
Wu
Shao-Yu
SY
Xu
Wei
W
Jin
Hong
H
Zhu
Zheng-Guang
ZG
Zhang
Jia-Jie
JJ
Pang
Jian-Xin
JX
Wu
Shu-Guang
SG
D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D002460:Cell Line; D003374:Coumarins / Q000494:pharmacology*; D017931:DNA Primers; D005455:Fluorescent Antibody Technique; D007249:Inflammation / Q000378:metabolism / Q000517:prevention & control*; D007375:Interleukin-1 / Q000235:genetics / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D051379:Mice; D016328:NF-kappa B / Q000037:antagonists & inhibitors* / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000235:genetics / Q000378:metabolism; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism
D003374:Coumarins; D017931:DNA Primers; D007375:Interleukin-1; D008070:Lipopolysaccharides; D016328:NF-kappa B; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; C441070:praeruptorin A; D052247:Nitric Oxide Synthase Type II