Rapid differentiation of Mycobacterium tuberculosis and M. bovis by high-resolution melt curve analysis.
Identification and characterization of the Mycobacterium tuberculosis strains are important for clinical and therapeutic management of tuberculosis. Real-time PCR with a high-resolution melt assay was found to improve the diagnostic process. The assay includes differentiation between M. tuberculosis and Mycobacterium bovis based on one single-nucleotide polymorphism (SNP) in the narGHJI and oxyR genes and determination of M. bovis based on the region of differences 1 (RD1). This assay correctly identified the 7 tested Mycobacterium reference strains and 52 clinical samples with a sensitivity of 2 pg DNA. This assay will help in prescribing adequate treatment and monitoring disease dynamics.
Ereqat
Suheir
S
Al-Quds Nutrition and Health Research Institute, Faculty of Medicine, Al-Quds University, Abu-Deis, PO Box 201760, West Bank, Palestine. sereqat@med.alquds.edu
Bar-Gal
Gila Kahila
GK
Nasereddin
Abedelmajeed
A
Azmi
Kifaya
K
Qaddomi
Sharif E
SE
Greenblatt
Charles L
CL
Spigelman
Mark
M
Abdeen
Ziad
Z
D001426:Bacterial Proteins / Q000235:genetics; D001431:Bacteriological Techniques / Q000379:methods*; D002648:Child; D002675:Child, Preschool; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics*; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D008969:Molecular Sequence Data; D009163:Mycobacterium bovis / Q000145:classification* / Q000235:genetics; D009169:Mycobacterium tuberculosis / Q000145:classification* / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D020641:Polymorphism, Single Nucleotide; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D044366:Transition Temperature; D014376:Tuberculosis / Q000175:diagnosis* / Q000382:microbiology*
D001426:Bacterial Proteins; D004269:DNA, Bacterial