[Development of new methods for the diagnosis of nasopharyngeal carcinoma].
OBJECTIVE
Development of new methods, ELISA and immunostrip test, for the diagnosis of nasopharyngeal carcinoma.


METHODS
The engineering purified antigens coat plate or absorb on nitrocellulose filter. The plate and diagnostic strips carrying antigens were used for detection of IgG antibody in the sera from nasopharyngeal carcinoma patients and outpatients patients.


RESULTS
127 cases sera from nasopharyngeal carcinoma patients were parallel detected TK/IgG antibody by ELISA and immunostrips. The TK/IgG antibody are all positive in the 127 cases of nasopharyngeal carcinoma patients. 55 cases show positive by ELISA, 58 cases positive by immunostrips in 247cases sera from outpatient. The antibody positive rate to early antigen p54 lower then to TK. Conclusion ELISA and imuunostrips are sensitive and specific means for detection of the IgG antibody to TK of EBV and the diagnosis of nasopharyngeal carcinoma.
Pi
Guo-Hua
GH
Beijng Mikrogen Biological Technology Co., LTD Beijing 100176, China.
Cheng
Dao-Sheng
DS
Zhou
Yi
Y
Zhang
Wen-Long
WL
D000914:Antibodies, Viral / Q000097:blood*; D002277:Carcinoma / Q000175:diagnosis* / Q000276:immunology / Q000821:virology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D020031:Epstein-Barr Virus Infections / Q000175:diagnosis* / Q000276:immunology / Q000821:virology; D004854:Herpesvirus 4, Human / Q000201:enzymology / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D009303:Nasopharyngeal Neoplasms / Q000175:diagnosis* / Q000276:immunology / Q000821:virology; D011934:Reagent Strips; D013937:Thymidine Kinase / Q000097:blood / Q000276:immunology; D014764:Viral Proteins / Q000097:blood / Q000276:immunology
D000914:Antibodies, Viral; D007074:Immunoglobulin G; D011934:Reagent Strips; D014764:Viral Proteins; D013937:Thymidine Kinase