title: [Genome sequencing and genetic analysis of a natural reassortant H5N1 subtype avian influenza virus possessing H9N2 internal genes].
abstract: Abstract:One H5N1 subtype avian influenza virus, A/duck/Shandong/009/2008 (Dk/SD/009/08), was isolated from apparently healthy domestic ducks in some live bird market in East China during our epidemiological surveillance. To investigate the genetic composition, Dk/SD/009/08 was subjected to genome sequencing. The amino acid motif of cleavage site was "PLRERRRK-R/GL", which was consistent with the characterization of the HPAIV. According to the newest unified nomenclature system of H5N1, Dk/SD/ 009/08 was classified into Clade 2.3.4. The BLAST results showed that four gene segments (HA, NA, NP and NS) had the highest nucleotide identities with H5N1 subtype AIVs whereas the remaining four (PB2, PB1, PA and M) displayed the closest relationship with H9N2 subtype. Therefore, Dk/SD/009/08 might be a natural reassortant virus. The phylogenetic analysis further indicated that G1-like H9N2 subtype AIVs which was prevalent mainly in quails of Southern China might provide the internal genes for Dk/ SD/009/08.
authors.affiliation: Key Laboratory of Animal Infectious Diseases, Yang Zhou University, Yang Zhou 225009, China. lucygu083@yahoo.com.cn
authors.forename: Min
authors.initials: M
authors.lastname: Gu
authors.forename: Wen-Bo
authors.initials: WB
authors.lastname: Liu
authors.forename: Jun-Ping
authors.initials: JP
authors.lastname: Cao
authors.forename: Yong-Zhong
authors.initials: YZ
authors.lastname: Cao
authors.forename: Xiao-Rong
authors.initials: XR
authors.lastname: Zhang
authors.forename: Da-Xin
authors.initials: DX
authors.lastname: Peng
authors.forename: Xiu-Fan
authors.initials: XF
authors.lastname: Liu
mesh_terms: D000818:Animals; D002642:Chick Embryo; D016679:Genome, Viral; D006801:Humans; D053124:Influenza A Virus, H5N1 Subtype / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D053127:Influenza A Virus, H9N2 Subtype / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D007251:Influenza, Human / Q000821:virology; D008969:Molecular Sequence Data; D010802:Phylogeny; D016865:Reassortant Viruses / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D011995:Recombination, Genetic; D014764:Viral Proteins / Q000235:genetics
chemical_list: D014764:Viral Proteins