title: [Synergistic interaction between MMP-3, VDR gene polymorphisms and occupational risk factors on lumbar disc degeneration].
abstract: OBJECTIVE
  To explore the synergistic interaction between MMP-3,VDR gene polymorphisms and occupational risk factors on lumbar disc degeneration.
  
  
  METHODS
  A case-control study including 178 cases of lumbar disc degeneration and 284 controls was carried out through questionnaire and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technology. Additive model was used to analyze the synergistic interaction between gene polymorphisms and occupational risk factors.
  
  
  RESULTS
  The non-conditional logistic regression analysis showed that bending/twisting, whole-body vibration, heavy physical workload, alleles 5A of MMP-3 (6A5A/5A5A) and A of VDR-Apa (AA/Aa) were significantly associated with lumbar disc degeneration(OR = 4.06, 8.96, 5.46, 1.96 and 1.70, respectively, P < 0.05). There were synergistic interactions between the mutation genotype 5A of MMP-3 and whole-body vibration exposure, between the mutation genotype 5A of MMP-3 and bending/twisting, and between the mutation genotype A of VDR-Apa and whole-body vibration exposure (SI: 13.27, 2.91 and 2.35 respectively, P < 0.05).
  
  
  CONCLUSIONS
  People with the mutation genotypes 5A of MMP-3 and/or A of VDR-Apa may have the increased risk of developing lumbar disc degeneration if they are exposed to whole-body vibration and/or bending/twisting.
authors.affiliation: Department of Occupational Health, School of Public Health, Fudan University, Shanghai 200032, China.
authors.forename: Han-Yan
authors.initials: HY
authors.lastname: Yuan
authors.forename: Ying
authors.initials: Y
authors.lastname: Tang
authors.forename: Ling
authors.initials: L
authors.lastname: Lei
authors.forename: Guo-Bing
authors.initials: GB
authors.lastname: Xiao
authors.forename: You-Xin
authors.initials: YX
authors.lastname: Liang
authors.forename: Sheng
authors.initials: S
authors.lastname: Wang
authors.forename: Zhao-Lin
authors.initials: ZL
authors.lastname: Xia
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D055959:Intervertebral Disc Degeneration / Q000235:genetics* / Q000473:pathology*; D008297:Male; D019278:Matrix Metalloproteinase 3 / Q000235:genetics*; D008875:Middle Aged; D016273:Occupational Exposure; D020641:Polymorphism, Single Nucleotide; D018167:Receptors, Calcitriol / Q000235:genetics*; D012307:Risk Factors
chemical_list: D018167:Receptors, Calcitriol; C511768:MMP3 protein, human; D019278:Matrix Metalloproteinase 3