Cytotoxicity of 9,11-dehydroergosterol peroxide isolated from Ganoderma lucidum and its target-related proteins.
The cytotoxicty of 9,11-dehydroergosterol peroxide (DHEP) isolated from the fruiting bodies of Ganoderma lucidum on HeLa cells was studied. DHEP treatment for 48 h inhibited the proliferation of HeLa human cervical carcinoma cells with an IC50-value of 8.58 +/- 0.98 microM. Morphological changes of DHEP-treated cells indicated that DHEP induced apoptosis in HeLa cells. To identify the cellular targets of DHEP, two-dimensional electrophoresis analysis was performed to compare the protein expression profiles of DHEP-treated cells with that of control cells. Proteins altered in expressional level after DHEP exposure were identified by MALDI-TOF MS/MS. The cytotoxic effect of DHEP was associated with regulated expression of 6 proteins. Stathmin 1 might be an important target-related protein of DHEP. The regulation of stathmin 1 by DHEP treatment was also confirmed by Western blotting.
Cui
Ya-Jun
YJ
Shanghai University of Chinese Traditional Medicine, Shanghai 201203, PR China.
Guan
Shu-Hong
SH
Feng
Li-Xing
LX
Song
Xiao-Yi
XY
Ma
Chao
C
Cheng
Chun-Ru
CR
Wang
Wen-Bo
WB
Wu
Wan-Ying
WY
Yue
Qing-Xi
QX
Liu
Xuan
X
Guo
De-An
DA
D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D004875:Ergosterol / Q000031:analogs & derivatives* / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D040901:Proteomics; D020940:Reishi / Q000737:chemistry*; D016914:Ribonuclease H / Q000032:analysis; D050777:Stathmin / Q000032:analysis
C543735:9,11-dehydroergosterol peroxide; D000970:Antineoplastic Agents; C493995:STMN1 protein, human; D050777:Stathmin; C091139:ribonuclease HII; D016914:Ribonuclease H; D004875:Ergosterol