Mechanism of uranium (VI) removal by two anaerobic bacterial communities.
The mechanism of uranium (VI) removal by two anaerobic bacterial consortia, recovered from an uncontaminated site (consortium A) and other from an uranium mine (consortium U), was investigated. The highest efficiency of U (VI) removal by both consortia (97%) occurred at room temperature and at pH 7.2. Furthermore, it was found that U (VI) removal by consortium A occurred by enzymatic reduction and bioaccumulation, while the enzymatic process was the only mechanism involved in metal removal by consortium U. FTIR analysis suggested that after U (VI) reduction, U (IV) could be bound to carboxyl, phosphate and amide groups of bacterial cells. Phylogenetic analysis of 16S rRNA showed that community A was mainly composed by bacteria closely related to Sporotalea genus and Rhodocyclaceae family, while community U was mainly composed by bacteria related to Clostridium genus and Rhodocyclaceae family.
Martins
Mónica
M
Centro de Ciências do Mar, Universidade do Algarve, FCT-DQF (edifício 8), Campus de Gambelas, 8005-139 Faro, Portugal.
Faleiro
Maria Leonor
ML
IBB - Centro de Biomedicina Molecular e Estrutural, Universidade do Algarve, FCT, Campus de Gambelas, 8005-139 Faro, Portugal.
da Costa
Ana M Rosa
AMR
Centro de Investigação em Química do Algarve, Universidade do Algarve, FCT, DQF, Campus de Gambelas, 8005-139 Faro, Portugal.
Chaves
Sandra
S
Universidade de Lisboa, Faculdade de Ciências, Centro de Biodiversidade, Genómica Integrativa e Funcional (BioFIG), Campus de FCUL, Campo Grande, 1749-016 Lisboa, Portugal.
Tenreiro
Rogério
R
Universidade de Lisboa, Faculdade de Ciências, Centro de Biodiversidade, Genómica Integrativa e Funcional (BioFIG), Campus de FCUL, Campo Grande, 1749-016 Lisboa, Portugal.
Matos
António Pedro
AP
Serviço de Anatomia Patológica, Hospital Curry Cabral, Lisboa, Portugal.
Costa
Maria Clara
MC
Centro de Ciências do Mar, Universidade do Algarve, FCT-DQF (edifício 8), Campus de Gambelas, 8005-139 Faro, Portugal. Electronic address: mcorada@ualg.pt.
D001421:Bacteria, Anaerobic / Q000145:classification / Q000378:metabolism*; D001483:Base Sequence; D017931:DNA Primers; D006863:Hydrogen-Ion Concentration; D046529:Microscopy, Electron, Transmission; D010084:Oxidation-Reduction; D010802:Phylogeny; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D014501:Uranium / Q000302:isolation & purification* / Q000378:metabolism; D014961:X-Ray Diffraction
D017931:DNA Primers; D014501:Uranium