title: Neuron-specific expression of atp6v0c2 in zebrafish CNS.
abstract: Vacuolar ATPase (V-ATPase) is a multi-subunit enzyme that plays an important role in the acidification of a variety of intracellular compartments. ATP6V0C is subunit c of the V(0) domain that forms the proteolipid pore of the enzyme. In the present study, we investigated the neuron-specific expression of atp6v0c2, a novel isoform of the V-ATPase c-subunit, during the development of the zebrafish CNS. Zebrafish atp6v0c2 was isolated from a genome-wide analysis of the zebrafish mib(ta52b) mutant designed to identify genes differentially regulated by Notch signaling. Whole-mount in situ hybridization revealed that atp6v0c2 is expressed in a subset of CNS neurons beginning several hours after the emergence of post-mitotic neurons. The ATP6V0C2 protein is co-localized with the presynaptic vesicle marker, SV2, suggesting that it is involved in neurotransmitter storage and/or secretion in neurons. In addition, the loss-of-function experiment suggests that ATP6V0C2 is involved in the control of neuronal excitability.
authors.affiliation: School of Life Science and Biotechnology, Kyungpook National University, Daegu, Korea.
authors.forename: Ah-Young
authors.initials: AY
authors.lastname: Chung
authors.forename: Myoung-Jin
authors.initials: MJ
authors.lastname: Kim
authors.forename: Dohyun
authors.initials: D
authors.lastname: Kim
authors.forename: Sangsu
authors.initials: S
authors.lastname: Bang
authors.forename: Sun Wook
authors.initials: SW
authors.lastname: Hwang
authors.forename: Chae Seung
authors.initials: CS
authors.lastname: Lim
authors.forename: Sanggyu
authors.initials: S
authors.lastname: Lee
authors.forename: Hae-Chul
authors.initials: HC
authors.lastname: Park
authors.forename: Tae-Lin
authors.initials: TL
authors.lastname: Huh
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002490:Central Nervous System / Q000033:anatomy & histology / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism*; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism*; D051880:Receptors, Notch / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D015398:Signal Transduction / Q000502:physiology; D025262:Vacuolar Proton-Translocating ATPases / Q000235:genetics / Q000378:metabolism*; D015027:Zebrafish / Q000033:anatomy & histology / Q000235:genetics / Q000378:metabolism; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D020033:Protein Isoforms; D021122:Protein Subunits; D051880:Receptors, Notch; D029961:Zebrafish Proteins; D025262:Vacuolar Proton-Translocating ATPases