The development of persistent duck hepatitis B virus infection can be prevented using antiviral therapy combined with DNA or recombinant fowlpoxvirus vaccines.
We recently reported the development of a successful post-exposure combination antiviral and "prime-boost" vaccination strategy using the duck hepatitis B virus (DHBV) model of human hepatitis B virus infection. The current study aimed to simplify the vaccination strategy and to test the post-exposure efficacy of combination therapy with the Bristol-Myers Squibb antiviral drug, entecavir (ETV) and either a single dose of DHBV DNA vaccines on day 0 post-infection (p.i.) or a single dose of recombinant fowlpoxvirus (rFPV-DHBV) vaccines on day 7 p.i. Whilst untreated control ducks infected with an equal dose of DHBV all developed persistent and wide spread DHBV infection of the liver, ducks treated with ETV combined with either the DHBV DNA vaccines on day 0 p.i. or the rFPV-DHBV vaccines on day 7 p.i. had no detectable DHBV-infected hepatocytes by day 14 p.i. and were protected from the development of persistent DHBV infection.
Feng
Feng
F
School of Molecular and Biomedical Science, University of Adelaide, Adelaide, SA 5005, Australia.
Teoh
Chee Quin
CQ
Qiao
Qiao
Q
Boyle
David
D
Jilbert
Allison R
AR
D000818:Animals; D004279:DNA, Viral / Q000097:blood / Q000302:isolation & purification; D004195:Disease Models, Animal; D004372:Ducks / Q000276:immunology; D005587:Fowlpox virus / Q000276:immunology; D018347:Hepadnaviridae Infections / Q000276:immunology / Q000517:prevention & control*; D006508:Hepatitis Antibodies / Q000097:blood / Q000276:immunology; D015611:Hepatitis B Virus, Duck / Q000276:immunology*; D006524:Hepatitis, Viral, Animal / Q000276:immunology / Q000517:prevention & control*; D022781:Hepatocytes / Q000276:immunology / Q000821:virology; D007117:Immunization, Secondary; D019444:Vaccines, DNA / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000276:immunology; D014759:Viral Envelope Proteins / Q000276:immunology; D014761:Viral Hepatitis Vaccines / Q000276:immunology*
D004279:DNA, Viral; D006508:Hepatitis Antibodies; C077433:Pre-S protein, Duck hepatitis B virus; D019444:Vaccines, DNA; D014614:Vaccines, Synthetic; D014759:Viral Envelope Proteins; D014761:Viral Hepatitis Vaccines