title: [Rat kidney mitochondrial damage induced by acute cadmium administration].
abstract: OBJECTIVE
  To evaluate effect of acute cadmium administration on mitochondria from rat kidney.
  
  
  METHODS
  24 male Sprague-Dawley (SD) rats were randomly divided into four groups. Four groups of rats were injected with saline, 0.6, 1.2, and 1.8 mg/kg weight subcutaneously, once daily, for 5 days, respectively. Ultrastructural change of rat kidney mitochondria was observed, and respiration function, membrane potential, mitochondria swelling, and superoxide level were determined.
  
  
  RESULTS
  Ultrastructural changes included matrix vacuolation, swelling and condensation of mitochondria. In group of 1.8 mg/kg body weight, the oxygen consumption rate during state 3 respiration [(6.25 +/- 0.61) nmol/L O2 x min(-1) x mg(-1)] and RCR value (2.45 +/- 0.23) were significantly lower than those of control group [(9.66 +/- 1.16) nmol/L O2 x min(-1) x mg(-1)] (P < 0.05), indicating respiration inhibition. The membrane potential and superoxide level of the same group were 85.89% +/- 3.82% and 116.33% +/- 3.06% of control values (P < 0.05), respectively.
  
  
  CONCLUSIONS
  Acute cadmium administration can cause rat kidney mitochondrial damage in a dose-effect manner, including inhibition of respiration, dissipation of membrane potential, swelling of mitochondria matrix. Such damage might be related to the increase of mitochondrial free radical.
authors.affiliation: School of Public Health/National Key Lab for Public Health Safety of MOE, Fudan University, Shanghai 200032, China.
authors.forename: Xiao-Hai
authors.initials: XH
authors.lastname: Pan
authors.forename: Kai
authors.initials: K
authors.lastname: Sun
authors.forename: Jia-Hu
authors.initials: JH
authors.lastname: Pan
authors.forename: Tai-Yi
authors.initials: TY
authors.lastname: Jin
mesh_terms: D000818:Animals; D002104:Cadmium / Q000633:toxicity*; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D008297:Male; D053078:Membrane Potential, Mitochondrial; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology*; D010100:Oxygen / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism; D023382:Toxicity Tests, Acute
chemical_list: D017382:Reactive Oxygen Species; D002104:Cadmium; D010100:Oxygen