Selected reaction monitoring applied to quantitative proteomics.
Proteomics is gradually shifting from pure qualitative studies (protein identification) to large-scale quantitative experiments, prompted by the growing need to analyze consistently and precisely a large set of proteins in biological samples. The selected reaction monitoring (SRM) technique is increasingly applied to quantitative proteomics because of its selectivity (two levels of mass selection), its sensitivity (non-scanning mode), and its wide dynamic range. This account describes the different steps in the design and the experimental setup of SRM experiments.
Kiyonami
Reiko
R
ThermoFisher Scientific, San Jose, CA, USA.
Domon
Bruno
B
D000595:Amino Acid Sequence; D053000:Analytic Sample Preparation Methods; D002853:Chromatography, Liquid; D006801:Humans; D007554:Isotopes; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D011485:Protein Binding; D040901:Proteomics / Q000379:methods*; D015203:Reproducibility of Results; D013223:Statistics as Topic; D013997:Time Factors
D007554:Isotopes; D010446:Peptide Fragments