Identification of osteoclastic factors in the nuclear envelope of mature, multinucleated osteoclasts.
Multinucleation is indispensable to the bone-resorbing activity of mature osteoclasts. Nevertheless, little is known about the regulatory networks among multi-nuclei in a single mature osteoclast. For this reason, we purified osteoclastic factors from the nuclear envelope by two-dimensional gel electrophoresis. Two annexin family proteins and ferritin light chain 1 protein were identified as osteoclastic candidates.
Youn
Min-Young
MY
Laboratory of Nuclear Signaling, Institute of Molecular and Cellular Biosciences, University of Tokyo, Tokyo, Japan.
Fujiyama-Nakamura
Sally
S
Takada
Ichiro
I
Imai
Yuuki
Y
Kato
Shigeaki
S
D000818:Animals; D017302:Annexins / Q000032:analysis*; D001052:Apoferritins / Q000032:analysis*; D001862:Bone Resorption / Q000209:etiology*; D002352:Carrier Proteins / Q000032:analysis*; D002460:Cell Line; D002478:Cells, Cultured; D015180:Electrophoresis, Gel, Two-Dimensional; D053263:Gene Regulatory Networks; D008264:Macrophages; D051379:Mice; D009685:Nuclear Envelope / Q000737:chemistry*; D010010:Osteoclasts / Q000737:chemistry*
D017302:Annexins; D002352:Carrier Proteins; D001052:Apoferritins