Tissue reaction to silver nanoparticles dispersion as an alternative irrigating solution.
BACKGROUND
Nanomaterials have been used to create new consumer products as well as applications for life sciences and biotechnology. The aim of this study was to evaluate the tissue response to implanted polyethylene tubes filled with fibrin sponge embedded with silver nanoparticles dispersion.


METHODS
Thirty rats received individually 4 polyethylene tubes filled with sponge embedded in 47 ppm, 23 ppm silver nanoparticles dispersion, 2.5% sodium hypochlorite, or with no embedding as control. The observation periods were 7, 15, 30, 60, and 90 days. After each period of time, 6 animals were killed, and the tubes and surrounding tissue were removed, fixed, and prepared to be analyzed in light microscope with glycol methacrylate embedding, 3-μm serial cutting, and hematoxylin-eosin stain. Qualitative and quantitative evaluations of the reactions were performed.


RESULTS
Both materials caused moderate reactions at 7 days. The response was similar to the control on the 15th day with 23 ppm silver nanoparticles dispersion and 2.5% sodium hypochlorite and on the 30th day with 47 ppm silver nanoparticles dispersion.


CONCLUSIONS
It was possible to conclude that silver nanoparticles dispersion was biocompatible especially in a lower concentration.
Gomes-Filho
João Eduardo
JE
Department of Endodontics, Araçatuba School of Dentistry, Univ. Estadual Paulista, Araçatuba, SP, Brazil. joao@foa.unesp.br
Silva
Fernando Oliveira
FO
Watanabe
Simone
S
Cintra
Luciano Tavares Angelo
LT
Tendoro
Karina Vanessa
KV
Dalto
Luana Godoy
LG
Pacanaro
Sara Vieira
SV
Lodi
Carolina Simonetti
CS
de Melo
Fernanda Fragoso Ferreira
FF
D000818:Animals; D002114:Calcinosis / Q000139:chemically induced; D003238:Connective Tissue / Q000187:drug effects*; D004337:Drug Carriers; D005337:Fibrin; D008297:Male; D053758:Nanoparticles / Q000633:toxicity*; D009336:Necrosis / Q000139:chemically induced; D020556:Neutrophil Infiltration; D051381:Rats; D017208:Rats, Wistar; D012388:Root Canal Irrigants / Q000633:toxicity*; D012834:Silver / Q000633:toxicity*; D012973:Sodium Hypochlorite / Q000633:toxicity
D004337:Drug Carriers; D012388:Root Canal Irrigants; D012834:Silver; D005337:Fibrin; D012973:Sodium Hypochlorite