Isolation of aggresomes and other large aggregates.
Upon permanent stresses and in various diseases, small protein aggregates may accumulate in cells and cause toxicity. A recently discovered protective system transports these aggregates to the centrosome location via microtubules, to form a large agglomerate of aggregates called the aggresomes. Here, we describe a newly developed method for isolating aggresomes. This principle can also be used for purification of other large structures and even organelles.
Meriin
Anatoli B
AB
Department of Biochemistry, Boston University School of Medicine, Boston, Massachusetts, USA.
Wang
Yan
Y
Sherman
Michael Y
MY
D000818:Animals; D001671:Biochemistry / Q000379:methods*; D018385:Centrosome / Q000378:metabolism*; D006801:Humans; D008869:Microtubule-Associated Proteins / Q000302:isolation & purification / Q000378:metabolism*; D046912:Multiprotein Complexes / Q000302:isolation & purification / Q000378:metabolism*; D055503:Protein Multimerization; D021381:Protein Transport
D008869:Microtubule-Associated Proteins; D046912:Multiprotein Complexes