Electrochemical detection of insertion/deletion mutations based on enhanced flexibility of bulge-containing duplexes on electrodes.
Ferrocene-modified DNA probes formed fully matched duplexes and bulge-containing ones with wild-type and insertion/deletion-type complements of clinical importance, respectively. Cyclic voltammetry measurements revealed that the bulge-containing duplexes showed an increased flexibility compared to the fully matched duplexes. The difference in the bending elasticity could be read out electrochemically by square wave voltammetry.
Chiba
Junya
J
Graduate School of Pharmaceutical Sciences, University of Toyama, Sugitani 2630, Toyama 930-0194, Japan. chiba@pha.u-toyama.ac.jp
Akaishi
Ayumi
A
Ikeda
Reona
R
Inouye
Masahiko
M
D001483:Base Sequence; D004247:DNA / Q000235:genetics*; D015342:DNA Probes / Q000737:chemistry*; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D005296:Ferrous Compounds / Q000737:chemistry*; D000075163:Metallocenes; D016254:Mutagenesis, Insertional; D017384:Sequence Deletion
D015342:DNA Probes; D005296:Ferrous Compounds; D000075163:Metallocenes; D004247:DNA; C004998:ferrocene