Changes of cytosolic Ca(2+) fluorescence intensity and plasma membrane calcium channels of maize root tip cells under osmotic stress.
The changes of cytosolic Ca(2+) fluorescence intensity and the activities of calcium channel of primary maize root tip cells induced by PEG6000 or abscisic acid (ABA) were studied by both confocal techniques and the whole-cell patch clamping in this study. The Ca(2+) fluorescence intensity increased while treated with PEG or ABA within 10 min, illuminating that Ca(2+) participated in the process of ABA signal transduction. For further proving the mechanism and origin of cytosolic Ca(2+) increase induced by PEG treatments, N,N,N',N'-tetraacetic acid (EGTA), Verapamil (VP) and Trifluoperazine (TFP) were added to the PEG solution in the experiments separately. The results showed that Ca(2+) fluorescence intensity induced by PEG was suppressed by both EGTA and VP obviously in the root tip cells. The Ca(2+) fluorescence intensity of plants changed after the addition of CaM inhibitor TFP while subjected to osmotic stress, which seemed to show that CaM participated in the process of signal transduction of osmotic stress too. The mechanism about it is unknown today. Further, a hyperpolarization-activated calcium permeable channel was recorded in plasma membrane of maize root tip cells. The Ca(2+) current (I(Ca)) intensity increased remarkably after PEG treatment, and the open voltage of the calcium conductance increased. Similar changes could be observed after ABA treatment, but the channel opened earlier and the current intensity was stronger than that of PEG treatment. The activation of calcium channel initiated by PEG strongly was inhibited by EGTA, VP or TFP respectively. The results revealed that Ca(2+) participated in the signals transduction process of osmotic stress, and the cytosolic free Ca(2+) increase by osmotic stress mainly came from the extracellular, and some came from the release of cytoplasmic calcium pool.
Liu
Zihui
Z
Plant Genetic Engineering Center of Hebei Province, Institute of Genetics and Physiology, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang, China.
Ma
Zhenyu
Z
Guo
Xiulin
X
Shao
Hongbo
H
Cui
Qiuhua
Q
Song
Weiyi
W
D000040:Abscisic Acid / Q000378:metabolism / Q000494:pharmacology; D000222:Adaptation, Physiological / Q000502:physiology*; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000187:drug effects / Q000378:metabolism*; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism; D004533:Egtazic Acid / Q000494:pharmacology; D005453:Fluorescence; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D009995:Osmosis; D018517:Plant Roots / Q000187:drug effects; D011092:Polyethylene Glycols / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects; D013312:Stress, Physiological; D014268:Trifluoperazine / Q000494:pharmacology; D014700:Verapamil / Q000494:pharmacology; D003313:Zea mays / Q000187:drug effects / Q000378:metabolism*
D015220:Calcium Channels; D014268:Trifluoperazine; D011092:Polyethylene Glycols; D004533:Egtazic Acid; D000040:Abscisic Acid; D014700:Verapamil; D002118:Calcium