Bioactive supramolecular hydrogel with controlled dual drug release characteristics.
Heparin, a powerful anticoagulant used for the prophylaxis of both surgical and medical thrombosis, was covalently incorporated into a supramolecular hydrogel network. For this attempt, heparin was first conjugated with amino-terminated poly(ethylene glycol) methyl ether by carbodiimide chemistry and then used to interact with α-cyclodextrin in aqueous solution. The rheological measurements and X-ray diffraction analyses were used to characterize the hydrogel formation. It was found that the gelation kinetics and hydrogel properties could be modulated by changing the amount of conjugated heparin or α-cyclodextrin. By circular dichroism analyses and in vitro release experiments, resultant hydrogel material was found to have a great potential as an injectable matrix for the encapsulation and sustained release of model protein drug (bovine serum albumin). By in vitro release, blood clotting, and hemolysis experiments, such a supramolecular hydrogel was also confirmed to have a controlled release profile for conjugated heparin and shows good anticoagulant and blood-compatible properties.
Ma
Dong
D
DSAPM Lab and PCFM Lab, Institute of Polymer Science, School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, China.
Tu
Kai
K
Zhang
Li-Ming
LM
D000818:Animals; D000925:Anticoagulants / Q000737:chemistry / Q000494:pharmacology; D001777:Blood Coagulation / Q000187:drug effects; D002417:Cattle; D002942:Circular Dichroism; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D016503:Drug Delivery Systems; D006461:Hemolysis / Q000187:drug effects; D006493:Heparin / Q000008:administration & dosage / Q000737:chemistry* / Q000494:pharmacology*; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D011092:Polyethylene Glycols / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000378:metabolism; D014961:X-Ray Diffraction; D047391:alpha-Cyclodextrins / Q000737:chemistry*
D000925:Anticoagulants; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D047391:alpha-Cyclodextrins; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols; D006493:Heparin; C032613:alpha-cyclodextrin