title: Cytotoxicity of denture adhesives.
abstract: Ten commercially available denture adhesives, nine soluble formulations (six creams, three powders) and one insoluble product (pad), were analyzed regarding the cytotoxicity profile in direct and indirect assays using L929 fibroblast cells. In the direct assay, fibroblasts were seeded over the surface of a thick adhesive gel (5%, creams; 2.5%, powders and pad). In the indirect assay, cells were cultured in the presence of adhesive extracts prepared in static and dynamic conditions (0.5-2%, creams; 0.25-1%, powders and pad). Cell toxicity was assessed for cell viability/proliferation (MTT assay) and cell morphology (observation of the F-actin cytoskeleton organization by confocal laser scanning microscopy). Direct contact of the L929 fibroblasts with the thick adhesive gels caused no, or only a slight, decrease in cell viability/proliferation. The adhesive extracts (especially those prepared in dynamic conditions) caused significantly higher growth inhibition of fibroblasts and, in addition, caused dose- and time-dependent effects, throughout the 6-72 h exposure time. Also, dose-dependent effects on cell morphology, with evident disruption of the F-actin cytoskeleton organization, were seen in the presence of most adhesives. In conclusion, the adhesives possessed different degrees of cytotoxicity, but similar dose- and time-dependent biological profiles.
authors.affiliation: Laboratório de Farmacologia e Biocompatibilidade Celular, Faculdade de Medicina Dentária, Universidade do Porto, Rua Dr. Manuel Pereira da Silva, 4200-393 Porto, Portugal.
authors.forename: Pedro Sousa
authors.initials: PS
authors.lastname: de Gomes
authors.forename: Maria Helena
authors.initials: MH
authors.lastname: Figueiral
authors.forename: Maria Helena R
authors.initials: MH
authors.lastname: Fernandes
authors.forename: Crispian
authors.initials: C
authors.lastname: Scully
mesh_terms: D000199:Actins / Q000187:drug effects; D000269:Adhesives / Q000633:toxicity*; D000818:Animals; D001672:Biocompatible Materials / Q000633:toxicity*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004396:Coloring Agents; D003470:Culture Media; D003599:Cytoskeleton / Q000187:drug effects; D003738:Dental Cements / Q000737:chemistry; D003781:Denture Retention; D004304:Dosage Forms; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000187:drug effects*; D005456:Fluorescent Dyes; D006863:Hydrogen-Ion Concentration; D008298:Maleates / Q000737:chemistry; D008422:Materials Testing; D051379:Mice; D018613:Microscopy, Confocal; D011095:Polyethylenes / Q000737:chemistry; D013696:Temperature; D013778:Tetrazolium Salts; D013844:Thiazoles; D013997:Time Factors
chemical_list: D000199:Actins; D000269:Adhesives; D001672:Biocompatible Materials; D004396:Coloring Agents; D003470:Culture Media; D003738:Dental Cements; D004304:Dosage Forms; D005456:Fluorescent Dyes; D008298:Maleates; D011095:Polyethylenes; C073208:Super Corega; D013778:Tetrazolium Salts; D013844:Thiazoles; C043105:poly(methyl vinyl ether-co-maleic anhydride); C022616:thiazolyl blue