title: Headspace analyses of ²H labeling of acetone: enabling studies of fatty acid oxidation in vivo.
abstract: We demonstrate that one can measure low levels of ²H labeling (e.g., <0.025% excess ²H) by exchanging hydrogen (deuterium) in water with acetone and subjecting samples to gas chromatography-pyrolysis-isotope ratio mass spectrometry. This analytical method circumvents the need to use typical off-line reduction methods that convert water to hydrogen gas prior to isotope ratio mass spectrometry or the need to purchase extra peripheral devices that would permit the direct analysis of water labeling. This method enables routine measurements of fatty acid oxidation in rodents; that is, one administers a ²H-labeled fatty acid(s) and then quantifies the production of ²H-labeled water.
authors.affiliation: Exploratory Biomarkers-Atherosclerosis, Merck Research Laboratories, Rahway, NJ 07065, USA.
authors.forename: Ablatt
authors.initials: A
authors.lastname: Mahsut
authors.forename: Sheng-Ping
authors.initials: SP
authors.lastname: Wang
authors.forename: David G
authors.initials: DG
authors.lastname: McLaren
authors.forename: Gowri
authors.initials: G
authors.lastname: Bhat
authors.forename: Kithsiri
authors.initials: K
authors.lastname: Herath
authors.forename: Paul L
authors.initials: PL
authors.lastname: Miller
authors.forename: Brian K
authors.initials: BK
authors.lastname: Hubbard
authors.forename: Douglas G
authors.initials: DG
authors.lastname: Johns
authors.forename: Stephen F
authors.initials: SF
authors.lastname: Previs
authors.forename: Thomas P
authors.initials: TP
authors.lastname: Roddy
mesh_terms: D000096:Acetone / Q000032:analysis*; D000818:Animals; D003903:Deuterium / Q000737:chemistry; D041961:Deuterium Exchange Measurement; D005227:Fatty Acids / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D007553:Isotope Labeling; D051379:Mice; D010084:Oxidation-Reduction; D014867:Water / Q000737:chemistry
chemical_list: D005227:Fatty Acids; D014867:Water; D000096:Acetone; D003903:Deuterium