title: Analysis of human synovial and bone marrow mesenchymal stem cells in relation to heat-inactivation of autologous and fetal bovine serums.
abstract: BACKGROUND
  Though sera are essential for Mesenchymal stem cells (MSCs), the effect of heat-inactivation remains unknown. Autologous human serum is recommended for clinical use; however, it is unclear whether differentiation potentials are maintained. To examine whether heat-inactivation of serum affected the proliferation and whether autologous human serum influenced their multipotentiality.
  
  
  METHODS
  After whole blood collection, human synovium and bone marrow were harvested. Nucleated cells were expanded with autologous human serum and FBS.
  
  
  RESULTS
  Heat-inactivation of autologous human serum enhanced proliferation of synovial MSCs. Heat-inactivation of each types of serum didn't affect calcification of synovial MSCs. The induction of calcification increased ALP activity, with the exception of bone marrow MSCs with autologous human serum. For adipogenesis of synovial MSCs, the Oil Red-O positive colony forming efficiency with autologous human serum was similar to or less than that with FBS.
  
  
  CONCLUSIONS
  These clarified the processing of human autologous serum and the influence of different sera for differentiation of synovial and bone marrow MSCs.
authors.affiliation: Section of Cartilage Regeneration, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan.
authors.forename: Akimoto
authors.initials: A
authors.lastname: Nimura
authors.forename: Takeshi
authors.initials: T
authors.lastname: Muneta
authors.forename: Koji
authors.initials: K
authors.lastname: Otabe
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Koga
authors.forename: Young-Jin
authors.initials: YJ
authors.lastname: Ju
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Mochizuki
authors.forename: Koji
authors.initials: K
authors.lastname: Suzuki
authors.forename: Ichiro
authors.initials: I
authors.lastname: Sekiya
mesh_terms: D000328:Adult; D000818:Animals; D001798:Blood Proteins / Q000737:chemistry / Q000502:physiology*; D001854:Bone Marrow Cells / Q000166:cytology / Q000502:physiology*; D002417:Cattle; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation / Q000502:physiology; D049109:Cell Proliferation; D002478:Cells, Cultured; D005260:Female; D005333:Fetus; D006358:Hot Temperature / Q000009:adverse effects*; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology*; D039902:Multipotent Stem Cells / Q000166:cytology / Q000502:physiology; D013583:Synovial Membrane / Q000166:cytology / Q000502:physiology*; D055815:Young Adult
chemical_list: D001798:Blood Proteins