[The study of the sensitive and specificity on HBV DNA replication by HBV-lP detecting].
OBJECTIVE
To detect HBV-LP and HBV DNA of the patients with hepatitis B, and to study the sensitive and specificity of HBV-LP detecting and its evaluate on for clinical application.


METHODS
The ELISA was used for HBV-LP detecting and RT-PCR for HBV DNA detecting.


RESULTS
The sensitive and specificity of HBV-LP and HBV DNA were 64.89%, 99.68%, 60.63% and 100% respectively (P > 0.05); +LR, -LR were 202.78, 60.63, 0.3522 and 0.3937, and there were significance between +LR of the detecting (P < 0.005).


CONCLUSIONS
The sensitive and specificity of HBV-LP and HBV DNA detecting are considerable, +LR of HBV-LP are higher comparing HBV DNA. The HBV-LP is better serology index for detecting replication of HBV DNA.
Shan
Ping-Nan
PN
The Laboratory Center of the Center Hospital of Shaoxing County, Zhejiang Shaoxing 312030, China.
Lu
Zhi-Yong
ZY
Xuan
Guo-Hong
GH
Weng
Li-Min
LM
Ru
Jin-Cheng
JC
Tan
Su-Hong
SH
Xu
De-Shun
DS
D000293:Adolescent; D000328:Adult; D004261:DNA Replication; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006509:Hepatitis B / Q000097:blood / Q000175:diagnosis* / Q000821:virology; D006515:Hepatitis B virus / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D008875:Middle Aged; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D014759:Viral Envelope Proteins / Q000097:blood*; D055815:Young Adult
C096362:L protein, hepatitis B virus; D014759:Viral Envelope Proteins