title: A combined A431 cell membrane chromatography and online high performance liquid chromatography/mass spectrometry method for screening compounds from total alkaloid of Radix Caulophylli acting on the human EGFR.
abstract: We have developed an online analytical method that combines A431 cell membrane chromatography (A431/CMC) with high performance liquid chromatography and mass spectrometry (LC/MS) for identifying active components from Radix Caulophylli acting on human EGFR. Retention fractions on A431/CMC model were captured onto an enrichment column and the components were directly analyzed by combining a 10-port column switcher with an LC/MS system for separation and preliminary identification. Using Sorafenib tosylate as a positive control, taspine and caulophine from Radix Caulophylli were identified as the active molecules which could act on the EGFR. This A431/CMC-online-LC/MS method can be applied for screening active components acting on EGFR from traditional Chinese medicines exemplified by Radix Caulophylli and will be of great utility in drug discovery using natural medicinal herbs as a source of novel compounds.
authors.affiliation: Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Sun
authors.forename: Jing
authors.initials: J
authors.lastname: Ren
authors.forename: Hui
authors.initials: H
authors.lastname: Du
authors.forename: Yanmin
authors.initials: Y
authors.lastname: Zhang
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.forename: Sicen
authors.initials: S
authors.lastname: Wang
authors.forename: Langchong
authors.initials: L
authors.lastname: He
mesh_terms: D000470:Alkaloids / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D028145:Caulophyllum / Q000737:chemistry*; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D066246:ErbB Receptors / Q000819:agonists / Q000037:antagonists & inhibitors / Q000378:metabolism*; D005449:Fluorenes / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D006801:Humans; D006860:Hydrogen Bonding; D013058:Mass Spectrometry / Q000379:methods*; D018517:Plant Roots / Q000737:chemistry; D011485:Protein Binding
chemical_list: D000470:Alkaloids; D004365:Drugs, Chinese Herbal; D005449:Fluorenes; C554063:caulophine; D066246:ErbB Receptors; C018394:taspine