[The preparation of P particle of the norovirus strain SZ9711 from China and its affinity analysis with human histo-blood group antigens in saliva].
OBJECTIVE
To study the binding profile of NV strain SZ9711 (GII-4) with human histo-blood group antigens (HBGAs).


METHODS
The P domain-encoding fragment was amplified by RT-PCR from the stain SZ9711 and cloned into the pGEX-4T-1 vector. The recombinant fusion protein was expressed in E. coli and purified using the column Sepharose 4B. The P protein was released by thrombin cleavage. The binding of P particles of SZ9711 and VA387 with the HBGAs were measured by saliva-based EIA method.


RESULTS
The expression of the recombinant fusion protein was shown by the SDS-PAGE, in which a 38 x 10(3)-P protein was obtained. Saliva-based EIA revealed that the P particle of SZ9711 bound to HBGAs in saliva similar to that of the strain VA387 reported previously. It bound strongly to saliva of type A, B and O(secretor) but did not interact with saliva of type O(non-secretor). Noteworthy, binding ability of SZ9711 P particle to type A saliva was lower than that of the VA387 P particle.


CONCLUSIONS
This is the first time that a P particle was prepared from a norovirus strain isolated in China and the binding ability of the P particle with HBGAs was analyzed. The result indicated the binding profile of the SZ9711 P particle was similar to that of VA387 reported previously. These data may be valuable in studying the relationship between noroviruses and their bindings to HGBA receptors.
Jin
Miao
M
Department of Viral Diarrhea, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.
He
Ya-Qing
YQ
Li
Hui-Ying
HY
Yang
Hong
H
Zhang
Hai-Long
HL
Qi
Rui
R
Yang
Xiao-Ke
XK
Fang
Shi-Song
SS
Tan
Ming
M
Duan
Zhao-Jun
ZJ
D001789:Blood Group Antigens / Q000378:metabolism*; D017250:Caliciviridae Infections / Q000378:metabolism* / Q000821:virology; D002681:China; D006801:Humans; D029322:Norovirus / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D012463:Saliva / Q000737:chemistry / Q000378:metabolism*; D014764:Viral Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*
D001789:Blood Group Antigens; D014764:Viral Proteins