title: Hepatoprotective effect of an immortal human fetal hepatic cell transplantation on CCL(4)-induced acute liver injury in mice.
abstract: Hepatocyte transplantation has been widely confirmed in the animal model experiments as an effective method for treatment of fulminant hepatic failure. However, the lack of donor organs remains a major problem. One solution is the development of transplantable hepatocytes. Herein we have transplanted intraperitoneally an established immortalized human fetal hepatic cell line (HL-7702) into CCl(4)-treated mice with acute liver injury to determine whether they provided life-saving metabolic support. The results showed lower levels of blood ammonia and higher content of liver albumin (P < .05) after HL-7702 transplantation versus nontransplanted controls at days 3 and 7. Histologic examination showed the transplantation group to be less affected at day 7 with no difference at day 14. In conclusion, an established immortal human fetal hepatic cell line may be a promising cell source providing life-saving metabolic support as a bioartificial liver device for the treatment of acute liver injury.
authors.affiliation: Center of Embryo Engineering and Technology, Nanjing Agricultural University, China.
authors.forename: Y B
authors.initials: YB
authors.lastname: Yan
authors.forename: H
authors.initials: H
authors.lastname: Song
authors.forename: B S
authors.initials: BS
authors.lastname: Zhong
authors.forename: Z Y
authors.initials: ZY
authors.lastname: Wang
authors.forename: S J
authors.initials: SJ
authors.lastname: Ying
authors.forename: F
authors.initials: F
authors.lastname: Wang
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D002251:Carbon Tetrachloride / Q000633:toxicity*; D002252:Carbon Tetrachloride Poisoning / Q000601:surgery; D002460:Cell Line; D005333:Fetus; D022781:Hepatocytes / Q000473:pathology / Q000637:transplantation*; D006801:Humans; D008099:Liver / Q000187:drug effects / Q000473:pathology*; D017114:Liver Failure, Acute / Q000601:surgery*; D019164:Liver, Artificial / Q000706:statistics & numerical data; D051379:Mice; D012709:Serum Albumin / Q000378:metabolism
chemical_list: D012709:Serum Albumin; D002251:Carbon Tetrachloride; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase