Quantitative analysis of sesquiterpene lactones in extract of Arnica montana L. by 1H NMR spectroscopy.
(1)H NMR spectroscopy was used as a method for quantitative analysis of sesquiterpene lactones present in a crude lactone fraction isolated from Arnica montana. Eight main components - tigloyl-, methacryloyl-, isobutyryl- and 2-methylbutyryl-esters of helenalin (H) and 11α,13-dihydrohelenalin (DH) were identified in the studied sample. The method allows the determination of the total amount of sesquiterpene lactones and the quantity of both type helenalin and 11α,13-dihydrohelenalin esters separately. Furthermore, 6-O-tigloylhelenalin (HT, 1), 6-O-methacryloylhelenalin (HM, 2), 6-O-tigloyl-11α,13-dihydrohelenalin (DHT, 5), and 6-O-methacryloyl-11α,13-dihydrohelenalin (DHM, 6) were quantified as individual components.
Staneva
Jordanka
J
Institute of Organic Chemistry with Center of Phytochemistry, Bulgarian Academy of Sciences, Acad G. Bonchev Str. Bl. 9, 1113 Sofia, Bulgaria. dani staneva@abv.bg
Denkova
Pavletta
P
Todorova
Milka
M
Evstatieva
Ljuba
L
D018650:Arnica / Q000378:metabolism*; D002623:Chemistry Techniques, Analytical; D002626:Chemistry, Pharmaceutical / Q000379:methods; D015195:Drug Design; D035264:Flowers / Q000737:chemistry; D007783:Lactones / Q000032:analysis* / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008956:Models, Chemical; D010936:Plant Extracts / Q000378:metabolism*; D012717:Sesquiterpenes / Q000032:analysis / Q000737:chemistry*; D045789:Sesquiterpenes, Guaiane
D007783:Lactones; D010936:Plant Extracts; D012717:Sesquiterpenes; D045789:Sesquiterpenes, Guaiane; C026745:plenolin; C001329:helenalin