MicroRNA microarrays on archive bone marrow core biopsies of leukemias--method validation.
Due to availability of bone marrow core biopsies (CB) in many pathology laboratories, we evaluated the quality and the biological information of the miRNA profiling using 9 acute lymphoblastic leukemia (ALL) and 9 chronic myeloid leukemia (CML) matched CB and bone marrow aspirates (BA). Technical replicates showed reproducible results across platforms and clustered together in hierarchical clustering analysis; and matched samples showed similar biological content having common differentially expressed miRNAs against the same control samples. We showed, that CBs, which have underwent decalcification in addition to formalin-fixation, are suitable for miRNA profiling.
Borze
Ioana
I
Department of Pathology, Haartman Institute and HUSLAB, University of Helsinki and Helsinki University Central Hospital, Helsinki, Finland.
Guled
Mohamed
M
Musse
Suad
S
Raunio
Anna
A
Elonen
Erkki
E
Saarinen-Pihkala
Ulla
U
Karjalainen-Lindsberg
Marja-Liisa
ML
Lahti
Leo
L
Knuutila
Sakari
S
D001706:Biopsy; D001853:Bone Marrow; D005557:Formaldehyde; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D007938:Leukemia / Q000235:genetics*; D035683:MicroRNAs / Q000302:isolation & purification*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016707:Tissue Fixation
D035683:MicroRNAs; D005557:Formaldehyde