title: Adverse reactions during transfusion of thawed haematopoietic progenitor cells from apheresis are closely related to the number of granulocyte cells in the leukapheresis product.
abstract: OBJECTIVE
  The infusion of thawed haematopoietic progenitor cells from apheresis (HPC-A) is associated with minor but frequent adverse reactions (ARs), which has been mainly attributed to dimethyl sulphoxide (DMSO). Nevertheless, other factors may play a role in the pathogenesis of such toxicity.
  
  
  METHODS
  The ARs on a cohort of 423 cryopreserved HPC-A infusions for 398 patients in HPC transplantation program were analysed.
  
  
  RESULTS
  ARs were reported in 105 graft infusions (24·8%) and most of them were graded as mild to moderate. The most frequently reported ARs were gastrointestinal and respiratory, and three patients presented epileptic seizure. The volume of DMSO/kg (P < 0·001), volume of red-blood-cells/kg (P = 0·02), number of nuclear cells (NCs)/kg (P <0·001) and number of granulocytes/kg (P<0·001) in the infused graft were significant in the univariate analysis for the occurrence of ARs. The amount of granulocytes/kg remained significant in the multivariate analysis (P<0·001). The grade of ARs also correlated with the amount of cryopreserved granulocytes.
  
  
  CONCLUSIONS
  The incidence and grade of ARs during infusion of cryopreserved HPC-A are related to the amount of granulocytes in the graft.
authors.affiliation: Blood and Tissue Bank, Granvia de l'Hospitalet, 201, 08908 l'Hospitalet de Llobregat, Barcelona, Spain. gmartin@bstcat.net
authors.forename: G A
authors.initials: GA
authors.lastname: Martín-Henao
authors.forename: P M
authors.initials: PM
authors.lastname: Resano
authors.forename: J M S
authors.initials: JM
authors.lastname: Villegas
authors.forename: P P
authors.initials: PP
authors.lastname: Manero
authors.forename: J M
authors.initials: JM
authors.lastname: Sánchez
authors.forename: M P
authors.initials: MP
authors.lastname: Bosch
authors.forename: A E
authors.initials: AE
authors.lastname: Codins
authors.forename: M S
authors.initials: MS
authors.lastname: Bruguera
authors.forename: L R
authors.initials: LR
authors.lastname: Infante
authors.forename: A P
authors.initials: AP
authors.lastname: Oyarzabal
authors.forename: R N
authors.initials: RN
authors.lastname: Soldevila
authors.forename: D C
authors.initials: DC
authors.lastname: Caiz
authors.forename: L M
authors.initials: LM
authors.lastname: Bosch
authors.forename: E C
authors.initials: EC
authors.lastname: Barbeta
authors.forename: J R G
authors.initials: JR
authors.lastname: Ronda
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D002675:Child, Preschool; D015925:Cryopreservation; D003451:Cryoprotective Agents / Q000494:pharmacology; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D005260:Female; D005767:Gastrointestinal Diseases / Q000453:epidemiology / Q000209:etiology*; D006098:Granulocytes; D006801:Humans; D007937:Leukapheresis; D007958:Leukocyte Count; D008297:Male; D008875:Middle Aged; D009369:Neoplasms / Q000628:therapy; D036102:Peripheral Blood Stem Cell Transplantation; D012120:Respiration Disorders / Q000453:epidemiology / Q000209:etiology*; D012640:Seizures / Q000453:epidemiology / Q000209:etiology*; D014182:Transplantation, Autologous; D014184:Transplantation, Homologous
chemical_list: D003451:Cryoprotective Agents; D004121:Dimethyl Sulfoxide