Isolation and identification of two steroid compounds from Oviductus Ranae.
In this study, cholest-5-en-3β,7β-diol and 3β-hydroxy-cholest-5-en-7-one were isolated from Oviductus Ranae by column chromatographies on Sephadex LH-20, octadecylsilyl (ODS) and pre high-performance liquid chromatography (HPLC). Their structures were elucidated on the basis of chemical and spectral analyses, including electrospray ionisation-mass spectrometry (ESI-MS) and nuclear magnetic resonance (NMR). The above two compounds were obtained from Oviductus Ranae for the first time.
Wang
Yongsheng
Y
College of Pharmacy, Jilin University, Changchun, P.R. China.
Wang
Lili
L
Hu
Yan
Y
Zhang
Lantong
L
Wang
Zhihan
Z
D000818:Animals; D002783:Cholestenones / Q000737:chemistry / Q000302:isolation & purification*; D005260:Female; D006888:Hydroxycholesterols / Q000737:chemistry / Q000302:isolation & purification*; D008419:Materia Medica / Q000737:chemistry*; D008516:Medicine, Chinese Traditional; D015394:Molecular Structure; D011896:Rana temporaria; D013256:Steroids / Q000737:chemistry / Q000302:isolation & purification*
C554703:3beta-hydroxy-cholest-5-en-7-one; D002783:Cholestenones; D006888:Hydroxycholesterols; D008419:Materia Medica; C539466:Oviductus Ranae; D013256:Steroids; C011724:cholest-5-en-3 beta,7 alpha-diol