title: Induction of SENP1 in endothelial cells contributes to hypoxia-driven VEGF expression and angiogenesis.
abstract: SENP1 (SUMO-specific protease 1) has been shown to be essential for the stability and activity of hypoxia-inducible factor 1 (HIF-1α) under hypoxia conditions. However, it is unknown how SENP1 activation and hypoxia signaling are coordinated in the cellular response to hypoxia. Here, we report the essential role of SENP1 in endothelial cells as a positive regulator of hypoxia-driven VEGF production and angiogenesis. SENP1 expression is increased in endothelial cells following exposure to hypoxia. Silencing of HIF-1α blocks SENP1 expression in cell response to hypoxia. Mutation of the hypoxia response element (HRE) on the Senp1 promoter abolishes its transactivation in response to hypoxia. Moreover, silencing of SENP1 expression decreases VEGF production and abrogates the angiogenic functions of endothelial cell. We also find that the elongated endothelial cells in embryonic brain section and vascular endothelial cells in embryonic renal glomeruli in Senp1(-/-) mice are markedly reduced than those in wild-type. Thus, these results show that hypoxia implies a positive feedback loop mediated by SENP1. This feedback loop is important in VEGF production, which is essential for angiogenesis in endothelial cells.
authors.affiliation: Department of Biochemistry and Molecular Cell Biology, Key Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of Education, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Xu
authors.forename: Yong
authors.initials: Y
authors.lastname: Zuo
authors.forename: Haizeng
authors.initials: H
authors.lastname: Zhang
authors.forename: Xunlei
authors.initials: X
authors.lastname: Kang
authors.forename: Fei
authors.initials: F
authors.lastname: Yue
authors.forename: Zhengfang
authors.initials: Z
authors.lastname: Yi
authors.forename: Mingyao
authors.initials: M
authors.lastname: Liu
authors.forename: Edward T H
authors.initials: ET
authors.lastname: Yeh
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Chen
authors.forename: Jinke
authors.initials: J
authors.lastname: Cheng
mesh_terms: D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000098:blood supply / Q000166:cytology / Q000378:metabolism; D047369:Chromatin Immunoprecipitation; D003546:Cysteine Endopeptidases; D004622:Embryo, Mammalian / Q000166:cytology / Q000378:metabolism; D010450:Endopeptidases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism* / Q000502:physiology*; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D000860:Hypoxia / Q000503:physiopathology*; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism; D007124:Immunoenzyme Techniques; D007678:Kidney Glomerulus / Q000098:blood supply / Q000166:cytology / Q000378:metabolism; D051379:Mice; D018919:Neovascularization, Physiologic; D011401:Promoter Regions, Genetic / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics; D020218:Response Elements; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014471:Umbilical Veins / Q000166:cytology / Q000378:metabolism; D042442:Vascular Endothelial Growth Factors / Q000378:metabolism*
chemical_list: D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D042442:Vascular Endothelial Growth Factors; D010450:Endopeptidases; C404512:SENP1 protein, human; D003546:Cysteine Endopeptidases; C501974:Senp1 protein, mouse