Golgi phosphoprotein 2 (GOLPH2/GP73/GOLM1) interacts with secretory clusterin.
Golgi phosphoprotein 2 (GOLPH2/GP73/GOLM1), a type-II Golgi transmembrane protein of unknown function, is up-regulated in many cancers. Its Golgi luminal domain is potentially the major functional domain. The goal of this study is to identify the proteins interacting with GOLPH2. Using secretory GOLPH2 (sGOLPH2, amino acid residues 55-401) as bait, secretory clusterin (sCLU) was identified as one interacting candidate by yeast two-hybrid screening, and the coiled-coil domain of GOLPH2 was found to be sufficient for interaction with sCLU. The interaction between GOLPH2 and sCLU was confirmed intracellularly and extracellularly. The intracellular co-localization of GOLPH2 and sCLU in Golgi was also shown. These results can help in understanding the biological and pathological significance of GOLPH2.
Zhou
Yan
Y
State Key Laboratory of Respiratory Diseases, Laboratory of Viral Immunology, Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, People's Republic of China.
Li
Leike
L
Hu
Longbo
L
Peng
Tao
T
D002460:Cell Line; D051152:Clusterin / Q000378:metabolism*; D005110:Extracellular Space / Q000378:metabolism; D006056:Golgi Apparatus / Q000378:metabolism; D006801:Humans; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D021381:Protein Transport; D013329:Structure-Activity Relationship; D013347:Subcellular Fractions / Q000378:metabolism; D020798:Two-Hybrid System Techniques
C495242:CLU protein, human; D051152:Clusterin; C410824:GOLM1 protein, human; D008565:Membrane Proteins