[Effect of visfatin on the expression of basic fibroblast growth factor in rat hepatic stellate cells].
OBJECTIVE
To investigate the effect of visfatin on the expression of basic fibroblast growth factor (FGF-2) in rat hepatic stellate cells (HSCs).


METHODS
Culture-activated rat HSCs were incubated with visfatin under different dose (50, 100, 200 ng/mL) for 6, 12, 24 and 36 hours respectively. Total RNA were collected at each time point. FGF-2 mRNA expression was detected by real-time reverse transcriptase polymerase chain reaction.


RESULTS
The mRNA expression of FGF-2 was dose-dependently up-regulated in rat HSCs when incubated with 50, 100 and 200 ng/mL visfatin (1.71 +/- 0.34 vs. 1, 2.25 +/- 0.26 vs. 1, 3.25 +/- 0.29 vs. 1, P < 0.05, respectively). FGF-2 mRNA expression also increased after 100 ng/mL visfatin treatment for 12 hours (1.68 +/- 0.31 vs. 1, P < 0.05), and the mRNA levels were time-dependently elevated from 24 hours to 36 hours (2.25 +/- 0.26 vs. 1, 2.95 +/- 0.32 vs. 1, P < 0.05, respectively).


CONCLUSIONS
Visfatin could induce FGF-2 mRNA expression in rat HSCs in a dose- and time-dependent manner. Visfatin might be involved in the pathogenesis of liver fibrosis.
Yuan
Cong
C
Department of Gastroenterology, West China Hospital, Sichuan University, Chengdu 610041, China.
Liang
Ning-Lin
NL
Liu
Xiao-Jing
XJ
Yang
Li
L
D000818:Animals; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D016222:Fibroblast Growth Factor 2 / Q000235:genetics / Q000378:metabolism*; D055166:Hepatic Stellate Cells / Q000187:drug effects* / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D008297:Male; D054409:Nicotinamide Phosphoribosyltransferase / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
D012333:RNA, Messenger; D016222:Fibroblast Growth Factor 2; D054409:Nicotinamide Phosphoribosyltransferase