title: Simultaneous detection of multiple single-nucleotide polymorphisms by a simple membrane chip.
abstract: Technologies that screen multiple single-nucleotide polymorphisms (SNPs) could be very valuable in predicting patients' susceptibilities to diseases or responses to therapeutic interventions. In this study, we developed a chip that can accurately detect four SNPs at same time. This chip is cost-effective and user-friendly because it uses a detection protocol analogous to dot blotting and does not require sophisticated instruments. To establish this chip, we designed and blotted onto a nylon membrane SNP-specific oligonucleotide probes for human angiotensinogen, cholesteryl ester transfer protein, and apolipoprotein E. This chip detected the corresponding SNPs harbored within the angiotensinogen, cholesteryl ester transfer protein, and apolipoprotein E sequences from 20 donors. Importantly, the SNPs detected by our chip matched exactly with the direct sequencing results, thereby highlighting the accuracy of this chip. In conclusion, our chip is a robust tool for multiple SNP screening and holds the potential to future refinement in detecting diseases-associating genes in patients.
authors.affiliation: Graduate Institute of Medical Genetics, Kaohsiung Medical University, Kaohsiung, Taiwan.
authors.forename: Yu-Hsiang
authors.initials: YH
authors.lastname: Yang
authors.forename: Ruei-Nian
authors.initials: RN
authors.lastname: Li
authors.forename: Shey-Cherng
authors.initials: SC
authors.lastname: Tzou
authors.forename: Jaw-Yuan
authors.initials: JY
authors.lastname: Wang
authors.forename: Hsin-Pai
authors.initials: HP
authors.lastname: Lee
authors.forename: Hsien-Chung
authors.initials: HC
authors.lastname: Wang
authors.forename: Fang-Ming
authors.initials: FM
authors.lastname: Chen
authors.forename: Yung-Hsin
authors.initials: YH
authors.lastname: Wang
authors.forename: Ming-Chia
authors.initials: MC
authors.lastname: Hsieh
authors.forename: Ming-Yii
authors.initials: MY
authors.lastname: Huang
authors.forename: Wei-Lung
authors.initials: WL
authors.lastname: Tseng
authors.forename: Shiu-Ru
authors.initials: SR
authors.lastname: Lin
authors.forename: Tian-Lu
authors.initials: TL
authors.lastname: Cheng
mesh_terms: D000808:Angiotensinogen / Q000235:genetics; D001057:Apolipoproteins E / Q000235:genetics; D053480:Cholesterol Ester Transfer Proteins / Q000235:genetics; D003362:Cost-Benefit Analysis; D015342:DNA Probes; D005820:Genetic Testing / Q000379:methods; D005838:Genotype; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000191:economics / Q000379:methods; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA
chemical_list: D001057:Apolipoproteins E; C506642:CETP protein, human; D053480:Cholesterol Ester Transfer Proteins; D015342:DNA Probes; D000808:Angiotensinogen