Evaluation of the interaction between β-cyclodextrin and psychotropic drugs by surface plasmon resonance assay with a Biacore ® system.
Phase-solubility studies have been used to evaluate the solubilizing effects of cyclodextrins (CDs) on lipophilic, water-insoluble drugs. However, large amounts of CDs and drugs are required to measure solubility by phase-solubility studies. Thus, more efficient approaches to evaluate the interaction of CDs with drugs are needed. Herein we introduce a method that evaluates the interaction between immobilized β-cyclodextrin and psychotropic drugs by surface plasmon resonance assay with a Biacore(®) system. Association constants and stoichiometries observed were generally consistent with values calculated by traditional methods, such as phase-solubility and continuous variation methods. Results showed that the analytical method using Biacore(®) was suitable to evaluate CD-drug interactions.
Kobayashi
Hiroaki
H
Department of Physical Chemistry, Hoshi University, 4-41, Ebara 2-chome, Shinagawa-ku, Tokyo 142-8501, Japan.
Endo
Tomohiro
T
Ogawa
Noriko
N
Nagase
Hiromasa
H
Iwata
Mariko
M
Ueda
Haruhisa
H
D000657:Amoxapine / Q000737:chemistry; D002623:Chemistry Techniques, Analytical; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D006801:Humans; D007700:Kinetics; D008956:Models, Chemical; D011485:Protein Binding; D011619:Psychotropic Drugs / Q000737:chemistry*; D015203:Reproducibility of Results; D012995:Solubility; D020349:Surface Plasmon Resonance / Q000295:instrumentation / Q000379:methods*; D014196:Trazodone / Q000737:chemistry; D047392:beta-Cyclodextrins / Q000737:chemistry*
D011619:Psychotropic Drugs; D047392:beta-Cyclodextrins; C031215:betadex; D000657:Amoxapine; D014196:Trazodone