Development of a polymerase chain reaction-restriction fragment length polymorphism assay for Leishmania major/Leishmania killicki/Leishmania infantum discrimination from clinical samples, application in a Tunisian focus.
Topoisomerase II gene of Leishmania genus was used to develop a molecular tool for detection and species differentiation of Leishmania from clinical samples. Identification was achieved by a polymerase chain reaction followed by digestion with 2 restriction endonucleases BstU1 and Taq1. Despite the relatively low sensitivity, it is able to differentiate between 3 complexes responsible for cutaneous leishmaniasis.
Haouas
Najoua
N
Laboratory of Parasitology-Mycology (99UR/08-05), Department of Clinical Biology B, Faculty of Pharmacy, Monastir, Tunisia. najoua.h@laposte.net
Garrab
Selma
S
Gorcii
Mohamed
M
Khorchani
Hassen
H
Chargui
Najla
N
Ravel
Christophe
C
Mezhoud
Habib
H
Babba
Hamouda
H
D001483:Base Sequence; D004250:DNA Topoisomerases, Type II / Q000235:genetics*; D016054:DNA, Protozoan / Q000032:analysis / Q000235:genetics; D006801:Humans; D007891:Leishmania / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D018314:Leishmania infantum / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D018320:Leishmania major / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D007896:Leishmaniasis / Q000175:diagnosis* / Q000469:parasitology; D016773:Leishmaniasis, Cutaneous / Q000175:diagnosis* / Q000469:parasitology; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D014416:Tunisia
D016054:DNA, Protozoan; D004250:DNA Topoisomerases, Type II