Overview of quantitative LC-MS techniques for proteomics and activitomics.
LC-MS is a useful technique for protein and peptide quantification. In addition, as a powerful tool for systems biology research, LC-MS can also be used to quantify post-translational modifications and metabolites that reflect biochemical pathway activity. This review discusses the different analytical techniques that use LC-MS for the quantification of proteins, their modifications and activities in a multiplex manner.
Timms
John F
JF
Cancer Proteomics Laboratory, EGA Institute for Women's Health, University College London, London, UK.
Cutillas
Pedro R
PR
D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D011499:Protein Processing, Post-Translational; D011506:Proteins / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D013194:Staining and Labeling
D011506:Proteins