[Effects of high mobility group box 1 in activating periodontal ligament fibroblasts to express cytokine].
OBJECTIVE
To investigate the influence of high mobility group box 1 (HMGB1) on the expression of interleukin 6 (IL-6), receptor activator of nuclear factor-kappa B ligand (RANKL) and osteoprotegerin (OPG) on periodontal ligament fibroblasts.


METHODS
Human periodontal ligament fibroblasts were stimulated with HMGB1 at concentrations of 10, 30, and 100 ng x mL(-1) for 24 h. RT-PCR and Western blot analysis were performed to check mRNA and protein expression of IL-6, RANKL and OPG on the cells.


RESULTS
The ratio of RANKL/OPG was increased at both mRNA and protein level after HMGB1 stimulation at 10, 30, 100 ng x mL(-1). Inflammatory cytokine IL-6 was upregulated by HMGB1 at the concentration of 100 ng x mL(-1).


CONCLUSIONS
Increased ratio of RANKL/OPG and IL-6 on periodontal ligament fibroblasts suggests that HMGB1 might play a role in the pathogenesis and progression of periodontal disease.
Sun
Qin-feng
QF
Dept. of Periodontology, School of Stomatology, Shandong University, Jinan 250012, China.
Xu
Yan
Y
Song
Hui
H
Hu
Ying-wei
YW
Yang
Pi-shan
PS
D002478:Cells, Cultured; D005347:Fibroblasts / Q000378:metabolism*; D024243:HMGB1 Protein / Q000378:metabolism*; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism*; D053244:Osteoprotegerin / Q000378:metabolism*; D010513:Periodontal Ligament / Q000166:cytology*; D053245:RANK Ligand / Q000378:metabolism*
D024243:HMGB1 Protein; C585491:HMGB1 protein, human; C508600:IL6 protein, human; D015850:Interleukin-6; D053244:Osteoprotegerin; D053245:RANK Ligand; C506030:TNFRSF11B protein, human; C505688:TNFSF11 protein, human