Differences in methylation at GATC sites in genomic DNA of Campylobacter coli from turkeys and swine.
A significant fraction (46/108, 43%) of swine isolates of Campylobacter coli but none of 81 isolates of C. coli from turkeys had genomic DNA that was resistant to digestion by MboI, suggesting methylation of adenines at GATC sites. No consistent association was noted between antimicrobial resistance and MboI resistance. Seven swine-associated multilocus sequence typing-based sequence types (STs) were detected among multiple isolates with MboI-resistant DNA. The data suggest host-associated DNA modification system(s) specific for adenine at GATC sites in C. coli from swine.
Wright
Sandra
S
North Carolina State University, Department of Food Science, 339 Schaub Hall, Raleigh, NC 27695, USA.
Wilson
Simone
S
Miller
William G
WG
Mandrell
Robert E
RE
Siletzky
Robin M
RM
Kathariou
Sophia
S
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002169:Campylobacter Infections / Q000382:microbiology / Q000662:veterinary; D017000:Campylobacter coli / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D019175:DNA Methylation; D004269:DNA, Bacterial / Q000235:genetics* / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D004352:Drug Resistance, Microbial; D011201:Poultry Diseases / Q000382:microbiology; D013552:Swine / Q000382:microbiology; D013553:Swine Diseases / Q000382:microbiology; D014422:Turkeys / Q000382:microbiology
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D015252:Deoxyribonucleases, Type II Site-Specific; C024405:GATC-specific type II deoxyribonucleases