Conversion of viable but nonculturable Vibrio cholerae to the culturable state by co-culture with eukaryotic cells.
VBNC Vibrio cholerae O139 VC-280 obtained by incubation in 1% solution of artificial sea water IO at 4°C for 74 days converted to the culturable state when co-cultured with CHO cells. Other eukaryotic cell lines, including HT-29, Caco-2, T84, HeLa, and Intestine 407, also supported conversion of VBNC cells to the culturable state. Conversion of VBNC V. cholerae O1 N16961 and V. cholerae O139 VC-280/pG13 to the culturable state, under the same conditions, was also confirmed. When VBNC V. cholerae O139 VC-280 was incubated in 1% IO at 4°C for up to 91 days, the number of cells converted by co-culture with CHO cells declined with each additional day of incubation and after 91 days conversion was not observed.
Senoh
Mitsutoshi
M
Collaborative Research Center of Okayama University for Infectious Diseases in India, Kolkata, India.
Ghosh-Banerjee
Jayeeta
J
Ramamurthy
Thandavarayan
T
Hamabata
Takashi
T
Kurakawa
Takashi
T
Takeda
Makoto
M
Colwell
Rita R
RR
Nair
G Balakrish
GB
Takeda
Yoshifumi
Y
D000818:Animals; D002460:Cell Line; D018920:Coculture Techniques; D006224:Cricetinae; D003412:Cricetulus; D017077:Culture Media, Conditioned; D006801:Humans; D014734:Vibrio cholerae / Q000166:cytology / Q000254:growth & development*
D017077:Culture Media, Conditioned