Identification of antibiotics using small molecule variable ligand display on gold nanoparticles.
Here we describe the use of simple 1-pot thiol exchange reactions to generate a library of mixed ligand-coated gold nanoparticles that was screened for antibiotic activity. A library of 120 nanoparticle conjugates was assembled and antibiotic activity toward E. coli was determined and found to depend upon the combination of thiols assembled onto the nanoparticles. The most active conjugate displayed 99.9% growth inhibition at 0.5 μM.
Bresee
Jamee
J
Department of Chemistry and Biochemistry, University of Colorado, Boulder, CO 80309, USA.
Maier
Keith E
KE
Melander
Christian
C
Feldheim
Daniel L
DL
D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development; D004927:Escherichia coli Infections / Q000188:drug therapy; D006046:Gold / Q000737:chemistry*; D008024:Ligands; D053768:Metal Nanoparticles / Q000737:chemistry*; D054852:Small Molecule Libraries / Q000737:chemistry* / Q000494:pharmacology*; D013438:Sulfhydryl Compounds / Q000737:chemistry
D000900:Anti-Bacterial Agents; D008024:Ligands; D054852:Small Molecule Libraries; D013438:Sulfhydryl Compounds; D006046:Gold