title: A preliminary study to understand the effect of Fasciola hepatica tegument on naïve macrophages and humoral responses in an ovine model.
abstract: Fasciola hepatica, the liver fluke, is a highly evolved endo-parasite that uses various mechanisms to evade the host immune system. The immunosuppressive capabilities of the parasite's excretory/secretory products have been well demonstrated by previous independent studies. However, the role of the parasite's tegument in the immune responses remains to be investigated. In this study, the effect of the tegument and other fractions of adult F. hepatica (excretory/secretory, liver fluke homogenate and liver fluke homogenate without tegument) in the activation of naïve macrophages in vitro was investigated using an ovine model. In addition, an immunoproteomic approach was used to investigate the characteristics of humoral antibody responses developed in sheep against the tegument fraction. The results indicated significantly increased arginase expression in macrophages incubated with the tegument and excretory/secretory fractions. Two dimensional gel electrophoresis of the tegument demonstrated approximately 100 protein spots, with only four of these spots were highly reactive with the positive serum as determined by 2-DE immunoblotting. These results give a preliminary indication that the liver fluke tegument may play role in avoiding hosts' protective immune responses against itself.
authors.affiliation: Veterinary Sciences Centre, School of Agriculture, Food Science and Veterinary Medicine, College of Life Sciences, University College Dublin, Belfield, Dublin 4, Ireland. orcun.hacariz@mam.gov.tr
authors.forename: O
authors.initials: O
authors.lastname: Haçarız
authors.forename: G
authors.initials: G
authors.lastname: Sayers
authors.forename: G
authors.initials: G
authors.lastname: Mulcahy
mesh_terms: D000818:Animals; D005210:Fasciola hepatica / Q000276:immunology*; D005211:Fascioliasis / Q000276:immunology / Q000662:veterinary*; D056724:Immunity, Humoral; D034582:Integumentary System / Q000502:physiology*; D008264:Macrophages / Q000502:physiology*; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology* / Q000469:parasitology