Reichenbachiella faecimaris sp. nov., isolated from a tidal flat, and emended descriptions of the genus Reichenbachiella and Reichenbachiella agariperforans.
A taxonomic study was carried out on two bacterial strains, PCP11(T) and PCP104, isolated from a tidal flat of the Yellow Sea, Korea. Comparative 16S rRNA gene sequence studies showed that these strains belonged to the family Cytophagaceae, phylum Bacteroidetes. Strains PCP11(T) and PCP104 shared 99.4 % sequence similarity and were related most closely to Reichenbachiella agariperforans KMM 3525(T) (95.8 and 96.0 % sequence similarity, respectively). Members of the genera Fulvivirga, Roseivirga, Fabibacter and Marinoscillum were the next closest relatives of the new isolates, with sequence similarities ≤ 91 %. The two isolates were Gram-staining-negative, strictly aerobic, gliding bacteria. They grew in the presence of 1-5 % NaCl, at pH 5.5-8.5 and at 4-35 °C. Strains PCP11(T) and PCP104 shared a number of physiological and biochemical properties with Reichenbachiella agariperforans KMM 3525(T), but they differed from this strain in the hydrolysis of biopolymers and in the production of carotenoid and flexirubin-type pigments. Both strains possessed iso-C(15 : 0), summed feature 4 (C(16 : 1)ω7c and/or iso-C(15 : 0) 2-OH) and C(15 : 0) as major cellular fatty acids. The major respiratory quinone was menaquinone 7 (MK-7). The G+C contents of the genomic DNA of strains PCP11(T) and PCP104 were 39.6 and 41.9 mol%, respectively. On the basis of phenotypic data and phylogenetic inference, it is proposed that the two isolates represent a novel species, Reichenbachiella faecimaris sp. nov., with strain PCP11(T) ( = KACC 14523(T)  = JCM 16588(T)) as the type strain. Emended descriptions of the genus Reichenbachiella and Reichenbachiella agariperforans are also proposed.
Cha
In-Tae
IT
Department of Microbiology and Biotechnology Research Institute, Chungbuk National University, Cheongju 361-763, Chungbuk, Republic of Korea.
Oh
Yong-Sik
YS
Department of Microbiology and Biotechnology Research Institute, Chungbuk National University, Cheongju 361-763, Chungbuk, Republic of Korea.
Park
Soo-Je
SJ
Department of Microbiology and Biotechnology Research Institute, Chungbuk National University, Cheongju 361-763, Chungbuk, Republic of Korea.
Park
Byoung-Joon
BJ
Department of Microbiology and Biotechnology Research Institute, Chungbuk National University, Cheongju 361-763, Chungbuk, Republic of Korea.
Lee
Je-Kwan
JK
Department of Microbiology and Biotechnology Research Institute, Chungbuk National University, Cheongju 361-763, Chungbuk, Republic of Korea.
Lim
Chae-Sung
CS
Department of Microbiology and Biotechnology Research Institute, Chungbuk National University, Cheongju 361-763, Chungbuk, Republic of Korea.
Park
A-Rum
AR
Department of Microbiology and Biotechnology Research Institute, Chungbuk National University, Cheongju 361-763, Chungbuk, Republic of Korea.
Yoo
Jae-Soo
JS
School of Electrical and Computer Engineering, Chungbuk National University, Cheongju 361-763, Chungbuk, Republic of Korea.
Lee
Dong-Hun
DH
Department of Microbiology and Biotechnology Research Institute, Chungbuk National University, Cheongju 361-763, Chungbuk, Republic of Korea.
Rhee
Sung-Keun
SK
Department of Microbiology and Biotechnology Research Institute, Chungbuk National University, Cheongju 361-763, Chungbuk, Republic of Korea.
Roh
Dong-Hyun
DH
Department of Microbiology and Biotechnology Research Institute, Chungbuk National University, Cheongju 361-763, Chungbuk, Republic of Korea.
D041964:Cytophagaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005227:Fatty Acids / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S