title: A rapid immunochromatographic test strip for detecting rabies virus antibody.
abstract: An immunochromatographic test strip (ICTS) for detecting antibodies to rabies virus was developed, using colloidal gold particles labeled with rabies virus glycoprotein as the tracer. The assay was evaluated using sera from dogs immunized with various commercial rabies vaccines, or from dogs in the clinics and sera from dogs immunized with vaccines against pathogens other than rabies virus, and negative sera from a wide variety of animal sources, including dogs, mice, and cats which had never been vaccinated. The ICTS was found to be highly specific for antibodies against rabies virus, with a detection limit of 0.5IU/ml as measured by the fluorescent antibody virus neutralization (FAVN) test. Compared with the FAVN test, the specificity and sensitivity of ICTS were 98.2% and 90.4%, respectively. There was an excellent agreement between results obtained by the ICTS and FAVN tests (kappa=0.888). Strips stored at 4°C in a plastic bag with a desiccant retained their specificity and sensitivity for at least 15 months, and strips stored at ambient temperature remained stable for 12 months. The immunochromatographic test strip may therefore be useful for clinical laboratories lacking specialized equipment and for diagnosis in the field for rapid detection of rabies virus-specific antibodies.
authors.affiliation: College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi An Road, Changchun, Jilin, China.
authors.forename: Hualei
authors.initials: H
authors.lastname: Wang
authors.forename: Na
authors.initials: N
authors.lastname: Feng
authors.forename: Songtao
authors.initials: S
authors.lastname: Yang
authors.forename: Chengyu
authors.initials: C
authors.lastname: Wang
authors.forename: Tiecheng
authors.initials: T
authors.lastname: Wang
authors.forename: Yuwei
authors.initials: Y
authors.lastname: Gao
authors.forename: Jianqing
authors.initials: J
authors.lastname: Su
authors.forename: Xuexing
authors.initials: X
authors.lastname: Zheng
authors.forename: Xiaoqiang
authors.initials: X
authors.lastname: Hou
authors.forename: Hainan
authors.initials: H
authors.lastname: Huang
authors.forename: Ruimei
authors.initials: R
authors.lastname: Yang
authors.forename: Xiaohuan
authors.initials: X
authors.lastname: Zou
authors.forename: Geng
authors.initials: G
authors.lastname: Huang
authors.forename: Xianzhu
authors.initials: X
authors.lastname: Xia
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D015153:Blotting, Western; D002415:Cats; D004285:Dogs; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D006023:Glycoproteins / Q000276:immunology; D017979:Gold Colloid; D007118:Immunoassay / Q000379:methods*; D051379:Mice; D009500:Neutralization Tests; D011818:Rabies / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D011819:Rabies Vaccines / Q000276:immunology; D011820:Rabies virus / Q000276:immunology*; D011934:Reagent Strips; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014764:Viral Proteins / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D006023:Glycoproteins; D017979:Gold Colloid; D011819:Rabies Vaccines; D011934:Reagent Strips; D014764:Viral Proteins