Plantlet regeneration from callus cultures of selected genotype of Aloe vera L.--an ancient plant for modern herbal industries.
Aloe vera L., a member of Liliaceae, is a medicinal plant and has a number of curative properties. We describe here the development of tissue culture method for high-frequency plantlet regeneration from inflorescence axis-derived callus cultures of sweet aloe genotype. Competent callus cultures were established on 0.8% agar-gelled Murashige and Skoog's (MS) basal medium supplemented with 6.0 mg l⁻¹ of 2,4-dichlorophenoxyacetic acid (2,4-D) and 100.0 mg l⁻¹ of activated charcoal and additives (100 mg l⁻¹ of ascorbic acid, 50.0 mg l⁻¹ each of citric acid and polyvinylpyrrolidone, and 25.0 mg l⁻¹ each of L-arginine and adenine sulfate). The callus cultures were cultured on MS medium containing 1.5 mg l⁻¹ of 2,4-D, 0.25 mg l⁻¹ of Kinetin (Kin), and additives with 4% carbohydrate source for multiplication and long-term maintenance of regenerative callus cultures. Callus cultures organized, differentiated, and produced globular embryogenic structures on MS medium with 1.0 mg l⁻¹ of 2,4-D, 0.25 mg l⁻¹ of Kin, and additives (50.0 mg l⁻¹ of ascorbic acid and 25.0 mg l⁻¹ each of citric acid, L-arginine, and adenine sulfate). These globular structures subsequently produced shoot buds and then complete plantlets on MS medium containing 1.0 mg l⁻¹ of 6-benzylaminopurine and additives. A hundred percent regenerated plantlets were hardened in the greenhouse and stored under an agro-net house/nursery. The regeneration system defined could be a useful tool not only for mass-scale propagation of selected genotype of A. vera, but also for genetic improvement of plant species through genetic transformation.
Rathore
Mangal S
MS
Biotechnology Center, Department of Botany, Jai Narain Vyas University, Jodhpur, Rajasthan 342005, India. mangalrathore@gmail.com
Chikara
J
J
Shekhawat
N S
NS
D015084:2,4-Dichlorophenoxyacetic Acid; D000225:Adenine / Q000378:metabolism; D000504:Aloe / Q000502:physiology; D001120:Arginine / Q000378:metabolism; D001205:Ascorbic Acid / Q000378:metabolism; D001709:Biotechnology / Q000379:methods; D019343:Citric Acid / Q000378:metabolism; D046508:Culture Techniques / Q000379:methods*; D005838:Genotype; D007701:Kinetin; D010937:Plant Growth Regulators; D018520:Plant Shoots / Q000235:genetics / Q000254:growth & development; D058439:Plant Somatic Embryogenesis Techniques / Q000379:methods*; D010946:Plants, Medicinal / Q000235:genetics / Q000254:growth & development; D011205:Povidone / Q000737:chemistry; D012038:Regeneration
D010937:Plant Growth Regulators; D015084:2,4-Dichlorophenoxyacetic Acid; D019343:Citric Acid; D001120:Arginine; D011205:Povidone; D000225:Adenine; D007701:Kinetin; D001205:Ascorbic Acid