Staining proteins: a simple method to increase the sensitivity of ellipsometric measurements in adsorption studies.
This communication describes a simple way to improve the sensitivity of spectroscopic ellipsometry, when applied to monitor the adsorption of proteins to solid surfaces. The method described herein is based on the reaction of a commercially available dye (Coomassie brilliant blue G) with the adsorbed proteins and the subsequent analysis by spectroscopic ellipsometry. In order to demonstrate the potential advantages of this method, the adsorption of bovine serum albumin to an antifouling coating was also investigated. According to our results, the modification with the dye significantly affects the optical properties of the adsorbed protein layer, which can be represented using a simple optical model (Lorentz). In general, the proposed modification increases the sensitivity of the detection by 2.5 ± 0.4-fold and enables the analysis of thin layers of adsorbed protein not obtainable by conventional methods. These results particularly reveal the importance of the proposed modification for the evaluation of low adsorbing substrates and antifouling coatings.
Nejadnik
M Reza
MR
Department of Chemistry, The University of Texas at San Antonio, One UTSA Circle, San Antonio, TX 78249, USA.
Garcia
Carlos D
CD
D000327:Adsorption; D000818:Animals; D002417:Cattle; D008954:Models, Biological; D055095:Optics and Photonics / Q000379:methods*; D011108:Polymers / Q000737:chemistry; D011506:Proteins / Q000032:analysis*; D012710:Serum Albumin, Bovine / Q000032:analysis; D013057:Spectrum Analysis; D013194:Staining and Labeling / Q000379:methods*; D013379:Substrate Specificity; D013997:Time Factors
D011108:Polymers; D011506:Proteins; D012710:Serum Albumin, Bovine