title: Chemoselective attachment of small molecule effector functionality to human adenoviruses facilitates gene delivery to cancer cells.
abstract: We demonstrate here a novel two-step "click" labeling process in which adenoviral particles are first metabolically labeled during production with unnatural azido sugars. Subsequent chemoselective modification allows access to viruses decorated with a broad array of effector functionality. Adenoviruses modified with folate, a known cancer-targeting motif, demonstrated a marked increase in gene delivery to a murine cancer cell line.
authors.affiliation: Department of Chemistry, State University of New York Stony Brook, New York 11790, USA.
authors.forename: Partha Sarathi
authors.initials: PS
authors.lastname: Banerjee
authors.forename: Philomena
authors.initials: P
authors.lastname: Ostapchuk
authors.forename: Patrick
authors.initials: P
authors.lastname: Hearing
authors.forename: Isaac
authors.initials: I
authors.lastname: Carrico
mesh_terms: D000256:Adenoviridae / Q000737:chemistry / Q000235:genetics*; D000818:Animals; D001386:Azides / Q000737:chemistry*; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D002470:Cell Survival; D005260:Female; D018014:Gene Transfer Techniques; D006801:Humans; D051379:Mice; D015394:Molecular Structure
chemical_list: D001386:Azides