Burkholderia vietnamiensis isolated from root tissues of Nipa Palm (Nypa fruticans) in Sarawak, Malaysia, proved to be its major endophytic nitrogen-fixing bacterium.
Root-associating bacteria of the nipa palm (Nypa fruticans), preferring brackish-water affected mud in Sarawak, Malaysia, were investigated. In a comparison of rhizobacterial microbiota between the nipa and the sago (Metroxylon sagu) palm, it was found that the nipa palm possessed a group of Burkholderia vietnamiensis as its main active nitrogen-fixing endophytic bacterium. Acetylene reduction by the various isolates of B. vietnamiensis was constant (44 to 68 nmol h(-1) in ethylene production rate) in soft gel medium containing 0.2% sucrose as sole carbon source, and the bacterium also showed motility and biofilm-forming capacity. This is the first report of endophytic nitrogen-fixing bacteria from nipa palm.
Tang
Sui-Yan
SY
Graduate School and Research Faculty of Agriculture, Hokkaido University, Sapporo, Japan.
Hara
Shintaro
S
Melling
Lulie
L
Goh
Kah-Joo
KJ
Hashidoko
Yasuyuki
Y
D000114:Acetylene / Q000378:metabolism; D028023:Arecaceae / Q000382:microbiology; D018441:Biofilms; D019117:Burkholderia / Q000166:cytology / Q000302:isolation & purification* / Q000378:metabolism; D007700:Kinetics; D008296:Malaysia; D009586:Nitrogen Fixation; D018517:Plant Roots / Q000382:microbiology*
D000114:Acetylene