Molecular cloning and expression of Chimonanthus praecox farnesyl pyrophosphate synthase gene and its possible involvement in the biosynthesis of floral volatile sesquiterpenoids.
Farnesyl pyrophosphate (FPP) synthase catalyzes the biosynthesis of FPP, which is the precursors of sesquiterpenoids such as floral scent volatiles, from isopentenyl pyrophosphate (IPP) and dimethylallyl pyrophosphate (DMAPP). cDNA encoding wintersweet (Chimonanthus praecox L.) FPP synthase was isolated by the RT-PCR and RACE methods. The deduced amino acid sequence showed a high identity to plant FPP synthases. Expression of the gene in Escherichia coli yielded FPPS activity that catalyzed the synthesis of FPP as a main product. Tissue-specific and developmental analyses of the mRNA levels of CpFPPS and volatile sesquiterpenoids levels in C. praecox flowers revealed that the FPPS may play a regulatory role in floral volatile sesquiterpenoids of wintersweet.
Xiang
Lin
L
Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, PR China.
Zhao
Kaige
K
Chen
Longqing
L
D000595:Amino Acid Sequence; D031237:Calycanthaceae / Q000235:genetics* / Q000378:metabolism; D018076:DNA, Complementary / Q000032:analysis; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D035264:Flowers / Q000378:metabolism*; D015870:Gene Expression; D017343:Genes, Plant; D051228:Geranyltranstransferase / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009822:Oils, Volatile / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012717:Sesquiterpenes / Q000378:metabolism*; D014170:Transformation, Genetic
D018076:DNA, Complementary; D009822:Oils, Volatile; D012333:RNA, Messenger; D012717:Sesquiterpenes; D051228:Geranyltranstransferase