Analytical performances of the Diazyme ADA assay on the Cobas® 6000 system.
OBJECTIVE
To evaluate the analytical performance of the Diazyme ADA assay on the Cobas® 6000 system for pleural fluid samples analysis.


METHODS
Imprecision, linearity, calibration curve stability, interference, and correlation studies were completed.


RESULTS
The Diazyme ADA assay demonstrated excellent precision (CV<4%) over the analytical measurement range (0.5-117 U/L). Bilirubin above 50 μmol/L and haemoglobin above 177 μmol/L interfered with the test, inducing a negative and a positive interference respectively. The Diazyme ADA assay correlated well with the Giusti method (r(2)=0.93) but exhibited a negative bias (~ -30%).


CONCLUSIONS
The Diazyme ADA assay on the Cobas® 6000 system represents a rapid, accurate, precise and reliable method for determination of ADA activity in pleural fluid samples.
Delacour
Hervé
H
Hôpital d'Instruction des Armées Bégin, Service de biochimie, Toxicologie, Pharmacologie Cliniques, 69, Saint Mandé, France. h_delacour@yahoo.fr
Sauvanet
Christophe
C
Ceppa
Franck
F
Burnat
Pascal
P
D000243:Adenosine Deaminase / Q000032:analysis*; D001681:Biological Assay / Q000295:instrumentation* / Q000379:methods / Q000592:standards; D001826:Body Fluids / Q000201:enzymology*; D002138:Calibration; D006801:Humans; D010996:Pleural Effusion / Q000201:enzymology*
D000243:Adenosine Deaminase