Simultaneous determination of disulfiram and bupropion in human plasma of alcohol and nicotine abusers.
An isocratic high-performance liquid-chromatographic method has been developed for the simultaneous determination of disulfiram and bupropion in human plasma samples. Analyses were carried out on a C(8) reversed-phase column using a mobile phase composed of 50% acetonitrile and 50% aqueous phosphate buffer, containing triethylamine. Diode-array detection was used, operating at a wavelength of 250 nm. For the clean-up of plasma samples, a solid phase extraction procedure, based on C(2) cartridges, was implemented. Extraction yields of the analytes were satisfactory, being always higher than 84%. The calibration curve was linear over the 5-500 ng mL(-1) plasma concentration range for both disulfiram and bupropion. The method showed a high sensitivity (limit of detection of 1.5 ng mL(-1)) and satisfactory precision, selectivity and accuracy. The application to human plasma samples obtained from some alcohol and nicotine abusers also gave good results.
Saracino
Maria Addolorata
MA
Department of Pharmaceutical Sciences, University of Bologna, Via Belmeloro 6, 40126 Bologna, Italy.
Marcheselli
Chiara
C
Somaini
Lorenzo
L
Gerra
Gilberto
G
De Stefano
Francesco
F
Pieri
Maria Chiara
MC
Raggi
Maria Augusta
MA
D000437:Alcoholism / Q000097:blood*; D016642:Bupropion / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004221:Disulfiram / Q000097:blood*; D006801:Humans; D015394:Molecular Structure; D014029:Tobacco Use Disorder / Q000097:blood*
D016642:Bupropion; D004221:Disulfiram