title: Role of substance P (SP) and calcitonin gene-related peptide (CGRP) in gibbon-ape-leukemia virus (GALV) transduction of CD34+ cells.
abstract: OBJECTIVE
  Optimization of transduction condition is an important goal to improve gene transduction. Therefore, we aimed to assess the effect of SP and/or CGRP as novel growth/transducing factors on the efficacy of CD34(+) transduction.
  
  
  METHODS
  CD34(+) cells were transduced with Gibbon-Ape-Leukemia virus (GALV), containing Neomycin gene. CD34(+) cells were transduced with GALV presence of SP and/or CGRP. Real Time PCR and colony formation assay (CFU-C) was performed.
  
  
  RESULTS
  Viral vectors titration on Hela cells indicated transduction efficiency of 1×10(6)CFU/ml. Real Time PCR of Neo and CFU-C showed stimulatory role of SP on gene transfer 5.9% and 14.84% compared to 3.6% and 12.58% in control group, while opposite role observed for CGRP 0.89% and 7.86%. Both SP and CGRP showed no significant effect in these assays.
  
  
  CONCLUSIONS
  This study showed including of SP in growth factor cocktail is beneficial for CD34(+) transduction, which could be applied to genetic modification procedures.
authors.affiliation: Department of Immunology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
authors.forename: Somayeh
authors.initials: S
authors.lastname: Shahrokhi
authors.forename: Kamran
authors.initials: K
authors.lastname: Alimoghaddam
authors.forename: Ardeshir
authors.initials: A
authors.lastname: Ghavamzadeh
mesh_terms: D018952:Antigens, CD34 / Q000378:metabolism*; D001773:Blood Cells / Q000187:drug effects* / Q000502:physiology*; D015740:Calcitonin Gene-Related Peptide / Q000494:pharmacology*; D002478:Cells, Cultured; D005312:Fetal Blood / Q000166:cytology; D018014:Gene Transfer Techniques; D006367:HeLa Cells; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000494:pharmacology; D017989:Leukemia Virus, Gibbon Ape / Q000235:genetics*; D013373:Substance P / Q000494:pharmacology*; D014161:Transduction, Genetic
chemical_list: D018952:Antigens, CD34; D036341:Intercellular Signaling Peptides and Proteins; D013373:Substance P; D015740:Calcitonin Gene-Related Peptide