Ligand dimerization programmed by hybridization to study multimeric ligand-receptor interactions.
Oligomerization of receptors induced or stabilized by polyvalent ligands is a fundamental mechanism in cellular recognition and signal transduction. Herein we report a general approach to encode complex peptide macrocycles with peptide nucleic acid (PNA) tags and program their oligomerization through hybridization as exemplified with a ligand binding to oligomeric DR5, a receptor of TRAIL cytokine.
Gorska
Katarzyna
K
Institut de Science et Ingenierie Supramoleculaires (ISIS), Université de Strasbourg-CNRS (UMR 7006), 8 allee Gaspard Monge, 67000 Strasbourg, France.
Beyrath
Julien
J
Fournel
Sylvie
S
Guichard
Gilles
G
Winssinger
Nicolas
N
D000595:Amino Acid Sequence; D019281:Dimerization; D008024:Ligands; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D020135:Peptide Nucleic Acids / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D055503:Protein Multimerization; D020836:Protein Structure, Quaternary; D053220:Receptors, TNF-Related Apoptosis-Inducing Ligand / Q000737:chemistry* / Q000378:metabolism*; D020349:Surface Plasmon Resonance
D008024:Ligands; D020135:Peptide Nucleic Acids; D053220:Receptors, TNF-Related Apoptosis-Inducing Ligand