Cellodextrin transport in yeast for improved biofuel production.
Fungal degradation of plant biomass may provide insights for improving cellulosic biofuel production. We show that the model cellulolytic fungus Neurospora crassa relies on a high-affinity cellodextrin transport system for rapid growth on cellulose. Reconstitution of the N. crassa cellodextrin transport system in Saccharomyces cerevisiae promotes efficient growth of this yeast on cellodextrins. In simultaneous saccharification and fermentation experiments, the engineered yeast strains more rapidly convert cellulose to ethanol when compared with yeast lacking this system.
Galazka
Jonathan M
JM
Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, CA 94720, USA.
Tian
Chaoguang
C
Beeson
William T
WT
Martinez
Bruno
B
Glass
N Louise
NL
Cate
Jamie H D
JH
D056804:Biofuels; D001692:Biological Transport; D018533:Biomass; D002475:Cellobiose / Q000378:metabolism; D002480:Cellulase / Q000378:metabolism; D002482:Cellulose / Q000031:analogs & derivatives* / Q000378:metabolism*; D003912:Dextrins / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism; D005285:Fermentation; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D005818:Genetic Engineering; D007700:Kinetics; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D009492:Neurospora crassa / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D001617:beta-Glucosidase / Q000378:metabolism
D056804:Biofuels; D003912:Dextrins; D005656:Fungal Proteins; D026901:Membrane Transport Proteins; D029701:Saccharomyces cerevisiae Proteins; D002475:Cellobiose; D000431:Ethanol; D002482:Cellulose; C068994:cellodextrin; D001617:beta-Glucosidase; D002480:Cellulase