Cardiac contractile dysfunction and apoptosis in streptozotocin-induced diabetic rats are ameliorated by garlic oil supplementation.
Previous studies have suggested that garlic oil could protect the cardiovascular system. However, the mechanism by which garlic oil protects diabetes-induced cardiomyopathy is unclear. In this study, streptozotocin (STZ)-induced diabetic rats received garlic oil (0, 10, 50, or 100 mg/kg of body weight) by gastric gavage every 2 days for 16 days. Normal rats without diabetes were used as control. Cardiac contractile dysfunction examined by echocardiography and apoptosis evaluated by terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) assay were observed in diabetic rat hearts. Additionally, a shift in cardiac myosin heavy chain (MHC) gene expression from α- to β-MHC isoform, decreased levels of superoxide dismutase-1 (SOD-1) and cardiac α-actin, and elevated cardiac thiobarbituric acid reactive substances (TBARS) and caspase- and p38-NFκB-leading apoptosis signaling activities were demonstrated in diabetic hearts. However, these diabetes-related cardiac dysfunctions were almost dose-dependently ameliorated by garlic oil administration. In conclusion, garlic oil possesses significant potential for protecting hearts from diabetes-induced cardiomyopathy.
Ou
Hsiu-Chung
HC
Department of Physical Therapy and Graduate Institute of Rehabilitation Science, China Medical University, 91 Hsueh-ShihRoad, Taichung 404, Taiwan.
Tzang
Bor-Show
BS
Chang
Mu-Hsin
MH
Liu
Cheng-Tzu
CT
Liu
Hui Wen
HW
Lii
Chong-Kuei
CK
Bau
Da-Tian
DT
Chao
Pei-Min
PM
Kuo
Wei-Wen
WW
D000498:Allyl Compounds / Q000008:administration & dosage*; D000818:Animals; D000975:Antioxidants / Q000032:analysis; D017209:Apoptosis; D020169:Caspases / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000150:complications* / Q000188:drug therapy; D058065:Diabetic Cardiomyopathies / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology; D004305:Dose-Response Relationship, Drug; D015870:Gene Expression; D020287:In Situ Nick-End Labeling; D008297:Male; D009200:Myocardial Contraction; D009206:Myocardium / Q000737:chemistry / Q000473:pathology; D018995:Myosin Heavy Chains / Q000235:genetics; D008517:Phytotherapy; D051381:Rats; D017208:Rats, Wistar; D013440:Sulfides / Q000008:administration & dosage*
D000498:Allyl Compounds; D000975:Antioxidants; D013440:Sulfides; C038491:allyl sulfide; D020169:Caspases; D018995:Myosin Heavy Chains