title: An alternative standard for Trolox-equivalent antioxidant-capacity estimation based on thiol antioxidants. Comparative 2,2'-azinobis[3-ethylbenzothiazoline-6-sulfonic acid] decolorization and rotational viscometry study regarding hyaluronan degradation.
abstract: Comparison of the effectiveness of antioxidant activity of three thiol compounds, D-penicillamine, reduced L-glutathione, and 1,4-dithioerythritol, expressed as a radical-scavenging capacity based on the two independent methods, namely a decolorization 2,2'-azinobis[3-ethylbenzothiazoline-6-sulfonic acid] assay and a rotational viscometry, is reported. Particular concern was focused on the testing of potential free-radical scavenging effects of thiols against hyaluronan degradation, induced by hydroxyl radicals. A promising, solvent-independent, antioxidative function of 1,4-dithioerythritol, comparable to that of a standard compound, Trolox(®), was confirmed by the 2,2'-azinobis[3-ethylbenzothiazoline-6-sulfonic acid] assay. The new potential antioxidant 1,4-dithioerythritol exhibited very good solubility in a variety of solvents (e.g., H(2)O, EtOH, and DMSO) and could be widely accepted and used as an effective antioxidant standard instead of a routinely used Trolox(®) on 2,2'-azinobis[3-ethylbenzothiazoline-6-sulfonic acid] assay.
authors.affiliation: Department of Glycochemistry, Institute of Chemistry, Slovak Academy of Sciences, Bratislava. eva.hrabarova@savba.sk
authors.forename: Eva
authors.initials: E
authors.lastname: Hrabárová
authors.forename: Katarína
authors.initials: K
authors.lastname: Valachová
authors.forename: Peter
authors.initials: P
authors.lastname: Rapta
authors.forename: Ladislav
authors.initials: L
authors.lastname: Soltés
mesh_terms: D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology; D052160:Benzothiazoles; D002839:Chromans / Q000737:chemistry* / Q000494:pharmacology; D004226:Dithioerythritol / Q000737:chemistry; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D016166:Free Radical Scavengers / Q000737:chemistry; D005978:Glutathione / Q000737:chemistry; D006820:Hyaluronic Acid / Q000737:chemistry* / Q000494:pharmacology; D017665:Hydroxyl Radical / Q000737:chemistry; D010084:Oxidation-Reduction; D010396:Penicillamine / Q000737:chemistry; D012995:Solubility; D012997:Solvents; D013053:Spectrophotometry; D013329:Structure-Activity Relationship; D013438:Sulfhydryl Compounds / Q000737:chemistry* / Q000494:pharmacology; D013451:Sulfonic Acids / Q000737:chemistry*; D013844:Thiazoles / Q000737:chemistry*; D014783:Viscosity
chemical_list: D000975:Antioxidants; D052160:Benzothiazoles; D002839:Chromans; D016166:Free Radical Scavengers; D012997:Solvents; D013438:Sulfhydryl Compounds; D013451:Sulfonic Acids; D013844:Thiazoles; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D017665:Hydroxyl Radical; D004226:Dithioerythritol; D006820:Hyaluronic Acid; D005978:Glutathione; D010396:Penicillamine; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid