A new fluorescent imaging of renal inflammation with RCP.
The objective of this study is to design a fluorescent imaging agent with R-Gel, one of the recombinant polymers (RCP), for renal inflammation. The R-Gel based on human type I collagen has multiple Arg-Gly-Asp (RGD) motifs which are ligands for some types of integrin receptors on the cell surface. After intravenous administration of R-Gel labeled by Cy7 of a fluorescent dye to three animal models of nephritis mousse, interstitial nephritis (by using UUO model mice), glomerulonephritis (HIGA mice), and ischemia-reperfusion injured kidney (I/R mice), the extent of fluorescent imaging at the renal inflammation was assessed. The Cy7-labeled R-Gel was accumulated in the inflammation site to a significantly greater extent than in the normal one at 24h after administration. The renal pattern of fluorescent imaging was similar to that of administration anti-Mac1 antibody. Taken together, it is conceivable that the R-Gel was targeted to macrophages infiltrated into the inflammation site of kidney.
Nakamura
Kentaro
K
Life Science Research Laboratories, FUJIFILM Corporation, 577 Ushijima, Kaisei-machi, Ashigarakami-gun, Kanagawa 258-8577, Japan. kentarou_a_nakamura@fujifilm.co.jp
Tabata
Yasuhiko
Y
D000818:Animals; D002232:Carbocyanines / Q000737:chemistry*; D024042:Collagen Type I / Q000737:chemistry; D004847:Epithelial Cells / Q000473:pathology; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D007668:Kidney / Q000473:pathology; D017737:Macrophages, Peritoneal / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009393:Nephritis / Q000175:diagnosis* / Q000473:pathology; D009842:Oligopeptides / Q000737:chemistry; D015427:Reperfusion Injury / Q000473:pathology
D002232:Carbocyanines; D024042:Collagen Type I; D005456:Fluorescent Dyes; D009842:Oligopeptides; C501744:indotricarbocyanine; C047981:arginyl-glycyl-aspartic acid