title: Production of antioxidant and antitumor metabolites by submerged cultures of Inonotus obliquus cocultured with Phellinus punctatus.
abstract: While Inonotus obliquus produces a diverse range of bioactive metabolites in its natural habitats, it accumulates less in its submerged cultures. We show here that coculture of I. obliquus with Phellinus punctatus resulted in less production of mycelial biomass but an increased accumulation of phenolic compounds, melanins, and lanostane-type triterpenoids. Metabolites increased in production by coculture include phelligridin C, phelligridin H, methyl inoscavin A, inoscavin C, inoscavin B, davallialactone, methyl davallialactone, foscoparianol D, 21,24-cyclopentalanosta-3β,21,25-triol-8-en, lanosta-7,9(11),23-triene-3β,22,25-triol, and inotodisaccharide and melanins. Metabolites from coculture also showed an increased potential for scavenging free radicals and inhibiting the proliferation of HeLa 229 cells. Davallialactone, methyl davallialactone, and minor phenolic components are the major contributors for scavenging DPPH and hydroxyl radical in monoculture, and phelligridin C, phelligridin H, methyl inoscavin A, inoscavin C, methyl davallialactone, foscoparianol D, and inotodisaccharide are those for scavenging the tested radicals in coculture. Lanostane-type triterpenoids indicated limited roles in scavenging free radicals. Nearly all the detected metabolites correlate positively with inhibiting proliferation of HeLa 229 cells. Thus, coculture of I. obliquus with other fungi seems to be a cost-effective strategy for upregulating biosynthesis of bioactive metabolites.
authors.affiliation: Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, Xuzhou Normal University, Xuzhou 221116, China. yyzw@xznu.edu.cn
authors.forename: Weifa
authors.initials: W
authors.lastname: Zheng
authors.forename: Yanxia
authors.initials: Y
authors.lastname: Zhao
authors.forename: Xin
authors.initials: X
authors.lastname: Zheng
authors.forename: Yubing
authors.initials: Y
authors.lastname: Liu
authors.forename: Shenyuan
authors.initials: S
authors.lastname: Pan
authors.forename: Yucheng
authors.initials: Y
authors.lastname: Dai
authors.forename: Fuming
authors.initials: F
authors.lastname: Liu
mesh_terms: D000970:Antineoplastic Agents / Q000378:metabolism* / Q000494:pharmacology; D000975:Antioxidants / Q000032:analysis / Q000378:metabolism*; D001487:Basidiomycota / Q000378:metabolism*; D049109:Cell Proliferation / Q000187:drug effects; D018920:Coculture Techniques; D046508:Culture Techniques / Q000379:methods*; D006367:HeLa Cells; D006801:Humans; D010636:Phenols / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D000975:Antioxidants; D010636:Phenols