A prognostic DNA signature for T1T2 node-negative breast cancer patients.
Predicting evolution of small node-negative breast carcinoma is a real challenge in clinical practice. The aim of this study was to search whether qualitative or quantitative DNA changes may help to predict metastasis of small node-negative breast carcinoma. Small invasive ductal carcinomas without axillary lymph node involvement (T1T2N0) from 168 patients with either good (111 patients with no event at 5 years after diagnosis) or poor (57 patients with early metastasis) outcome were analyzed with comparative genomic hybridization (CGH) array. A CGH classifier, identifying low- and high-risk groups of metastatic recurrence, was established in a training set of 78 patients, then validated, and compared with clinicopathological parameters in a distinct set of 90 patients. The genomic status of regions located on 2p22.2, 3p23, and 8q21-24 and the number of segmental alterations were defined in the training set to classify tumors into low- or high-risk groups. In the validation set, in addition to estrogen receptors and grade, this CGH classifier provided significant prognostic information in multivariate analysis (odds ratio, 3.34; 95% confidence interval 1.01-11.02; P = 4.78 × 10(-2), Wald test). This study shows that tumor DNA contains important prognostic information that may help to predict metastasis in T1T2N0 tumors of the breast.
Gravier
Eléonore
E
Department of Translational Research, Institut Curie, 26 rue d’Ulm, Paris, France.
Pierron
Gaëlle
G
Vincent-Salomon
Anne
A
Gruel
Nadège
N
Raynal
Virginie
V
Savignoni
Alexia
A
De Rycke
Yann
Y
Pierga
Jean-Yves
JY
Lucchesi
Carlo
C
Reyal
Fabien
F
Fourquet
Alain
A
Roman-Roman
Sergio
S
Radvanyi
François
F
Sastre-Garau
Xavier
X
Asselain
Bernard
B
Delattre
Olivier
O
D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology*; D018270:Carcinoma, Ductal, Breast / Q000235:genetics* / Q000473:pathology* / Q000556:secondary; D016022:Case-Control Studies; D002889:Chromosomes, Human, Pair 2 / Q000235:genetics; D002893:Chromosomes, Human, Pair 3 / Q000235:genetics; D002898:Chromosomes, Human, Pair 8 / Q000235:genetics; D055028:Comparative Genomic Hybridization; D020732:Cytogenetic Analysis; D004273:DNA, Neoplasm / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D008198:Lymph Nodes / Q000473:pathology*; D008207:Lymphatic Metastasis; D009362:Neoplasm Metastasis; D009364:Neoplasm Recurrence, Local; D017063:Outcome Assessment, Health Care; D011379:Prognosis; D011960:Receptors, Estrogen; D018570:Risk Assessment
D004273:DNA, Neoplasm; D011960:Receptors, Estrogen