Characterization of oil bodies in adlay (Coix lachryma-jobi L).
Oil bodies were observed in cells of both embryo and aleurone layers of mature adlay grains (Coix lachryma-jobi L. var. ma-yuen Stapf). Stable oil bodies were successfully isolated from the adlay grains. Thin-layer chromatography revealed that the contents stored in the adlay oil bodies were mainly neutral lipids (>90% triacylglycerols and about 5% diacylglycerols). The integrity of the isolated oil bodies was presumably maintained via electronegative repulsion and steric hindrance provided by their surface proteins. Immunological cross-recognition using antibodies against sesame oil-body proteins indicated that two oleosin isoforms (termed oleosin-H and oleosin-L) and one caleosin were present in the adlay oil bodies. Full-length cDNA fragments encoding these three unique oil-body proteins were obtained by PCR cloning. MALDI-MS analyses confirmed that the three full-length cDNA fragments encoded the two oleosin isoforms and one caleosin observed in the oil bodies isolated from the adlay grains.
Lu
Hsi-Chi
HC
Department of Food Science, Tunghai University, Taichung, Taiwan.
Jiang
Pei-Luen
PL
Hsu
Light R C
LR
Chyan
Chia-Lin
CL
Tzen
Jason T C
JT
D002135:Calcium-Binding Proteins; D002855:Chromatography, Thin Layer; D031742:Coix / Q000737:chemistry / Q000648:ultrastructure*; D018076:DNA, Complementary; D002479:Inclusion Bodies / Q000737:chemistry*; D008055:Lipids / Q000032:analysis; D008969:Molecular Sequence Data; D010938:Plant Oils / Q000032:analysis*; D010940:Plant Proteins; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D002135:Calcium-Binding Proteins; D018076:DNA, Complementary; D008055:Lipids; D010938:Plant Oils; D010940:Plant Proteins; C402836:caleosin protein, Sesamum indicum; C110161:oleosin protein, Sesamum indicum