title: Particle geometry affects differentially substrate composition and enzyme profiles by Pleurotus ostreatus growing on sugar cane bagasse.
abstract: The growth of Pleurotus ostreatus was analyzed on three particle sizes of sugar cane bagasse: 0.92 mm and 1.68 mm in diameter, in addition to heterogeneous fibers (average 2.9 mm in diameter). Specific growth rate on heterogeneous particles was lower (μ=0.043 h(-1)), although soluble protein production was maximal (809 μg/g dry wt). Higher μ values were reached on the other two particles sizes (0.049-0.05 h(-1)) with less soluble protein (500 μg/g dry wt). Xylanases and laccases were favored in heterogeneous particles; while the highest selectivity for xylanases over cellulases was observed in 1.68 mm particles, corresponding with the maximal hemicellulose breakdown. Lignin and cellulose were preferentially degraded in smallest particles. This study shows that the geometrical ratio, shape and size of sugar cane bagasse fibers strongly influence packing density for SSF substrate, with an impact in the production of extracellular enzymes, growth rates and composition changes in substrate.
authors.affiliation: División de Química y Bioquímica, Tecnológico de Estudios Superiores Ecatepec., Ecatepec 55210, Mexico.
authors.forename: Isabel
authors.initials: I
authors.lastname: Membrillo
authors.forename: Carmen
authors.initials: C
authors.lastname: Sánchez
authors.forename: Marcos
authors.initials: M
authors.lastname: Meneses
authors.forename: Ernesto
authors.initials: E
authors.lastname: Favela
authors.forename: Octavio
authors.initials: O
authors.lastname: Loera
mesh_terms: D018533:Biomass; D002480:Cellulase / Q000378:metabolism; D002482:Cellulose / Q000494:pharmacology*; D043364:Endo-1,4-beta Xylanases / Q000378:metabolism; D005374:Filtration; D042845:Laccase / Q000378:metabolism; D010316:Particle Size; D020076:Pleurotus / Q000201:enzymology* / Q000254:growth & development*; D031786:Saccharum / Q000737:chemistry*; D012995:Solubility / Q000187:drug effects; D013379:Substrate Specificity / Q000187:drug effects
chemical_list: D002482:Cellulose; C027433:bagasse; D042845:Laccase; D002480:Cellulase; D043364:Endo-1,4-beta Xylanases