Influence of ionic interactions on essential oil and phenolic diterpene composition of Dalmatian sage (Salvia officinalis L.).
The potential of four essential cations (K(+), Ca(2+), Mg(2+) and Fe(2+)) to alleviate salt toxicity was studied in sage (Salvia officinalis L.) plants grown in pots. Two concentrations of the following chloride salts: KCl, CaCl₂, MgCl₂ and FeCl₃, were used together with 100mM NaCl to study the effects of these nutrients on plant growth, leaf essential oils (EOs) and phenolic diterpenes composition. The sage plants accumulated Na(+) in their leaves (includers); this has affected secondary metabolites' biosynthesis. Treatment with 100mM NaCl slightly decreased borneol and viridiflorol, while increased manool concentrations. Addition of KCl, CaCl₂ and MgCl₂ increased considerably in a dose-dependent manner the oxygen-containing monoterpenes (1.8-cineole, camphor, β-thujone and borneol) in 100mM NaCl-treated sage. Whereas, the contents of viridiflorol decreased further with the addition of KCl in 100mM NaCl-treated sage. Our results suggest that the changes in EOs composition were more related to K(+) and Ca(2+) availability than to Na(+) toxicity. Furthermore, treatment with NaCl decreased by 50% carnosic acid (CA), a potent antioxidant, content in the leaves. K(+) and Ca(2+) promoted the accumulation of CA and its methoxylated form (MCA) in the leaves. The concentration of CA was positively correlated with leaf K(+) (r=0.56, P=0.01) and Ca(2+) (r=0.44, P=0.05) contents. It appears that different salt applications in combination with NaCl treatments had a profound effect on EOs and phenolic diterpene composition in sage. Therefore, ionic interactions may be carefully considered in the cultivation of this species to get the desired concentrations of these secondary metabolites in leaf extracts.
Tounekti
Taieb
T
Laboratory of Biotechnology Applied to Crop Improvement, Faculty of Sciences of Gabes, Unversity of Gabès, Cité Erriadh, Zrig 6072 Gabès, Tunisia.
Munné-Bosch
Sergi
S
Vadel
A M
AM
Chtara
Chaker
C
Khemira
Habib
H
D045784:Abietanes / Q000378:metabolism*; D000975:Antioxidants / Q000378:metabolism; D002118:Calcium / Q000378:metabolism / Q000494:pharmacology; D002712:Chlorides / Q000378:metabolism / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D009822:Oils, Volatile / Q000378:metabolism*; D010636:Phenols / Q000378:metabolism*; D010936:Plant Extracts / Q000378:metabolism*; D018515:Plant Leaves / Q000187:drug effects / Q000378:metabolism; D011188:Potassium / Q000378:metabolism / Q000494:pharmacology; D055049:Salt Tolerance / Q000187:drug effects / Q000502:physiology*; D012492:Salts / Q000378:metabolism / Q000494:pharmacology*; D027543:Salvia officinalis / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D012964:Sodium / Q000378:metabolism / Q000633:toxicity; D012965:Sodium Chloride / Q000378:metabolism / Q000633:toxicity*; D013312:Stress, Physiological; D014131:Trace Elements / Q000378:metabolism / Q000494:pharmacology
D045784:Abietanes; D000975:Antioxidants; D002712:Chlorides; D009822:Oils, Volatile; D010636:Phenols; D010936:Plant Extracts; D012492:Salts; D014131:Trace Elements; D012965:Sodium Chloride; D012964:Sodium; C018381:salvin; D011188:Potassium; D002118:Calcium