Quantitative, label-free and site-specific monitoring of molecular recognition: a multivariate resonance Raman approach.
A site-specific and quantitative approach for label-free monitoring of molecular recognition is presented. Specifically, the binding site of an artificial receptor is probed selectively by UVRR spectroscopy. The ligand binding constant can be determined by non-negative matrix factorization.
Niebling
Stephan
S
University of Osnabrück, Department of Physics, Barbarastr. 7, 49069 Osnabrück, Germany.
Kuchelmeister
Hannes Y
HY
Schmuck
Carsten
C
Schlücker
Sebastian
S
D001665:Binding Sites; D002264:Carboxylic Acids / Q000737:chemistry*; D008024:Ligands; D009842:Oligopeptides / Q000737:chemistry*; D018000:Receptors, Peptide / Q000737:chemistry*; D013059:Spectrum Analysis, Raman
D002264:Carboxylic Acids; D008024:Ligands; D009842:Oligopeptides; D018000:Receptors, Peptide