title: [In vitro study for detecting the effects of Bazhen decoction on proliferation and activation of T lymphocytes].
abstract: This study was designed to detect the effects of Bazhen decoction on the proliferation and activation of T lymphocytes. T lymphocytes were isolated. The effects of Bazhen decoction on the prolifertion and activation of T lymphocytes, and on the secretion of IFN-gamma IL-2 from T lymphocytes, were detected by MTT, Flow cytometry and ELISA. Results showed that proliferation of T lymphocytes was promoted significantly by different concentration of Bazhen decoction; and after different time, the relationships of "the longer the time, the higher the concentration, and the more enhanced the proliferation" came to be apparent. After 72h, T lymphocytes were activated with different concentration of Bazhen decoction, the rate of CD69+ T cells increased signficantly, the secretion of IFN-gamma and IL-2 increased signficicantly, and the effect exhibited a dose-dependent manner. The results of this epxeriment indicated that Bazhen decoction could promote the proliferation and activation of T lymphocytes.
authors.affiliation: Medical College of Hebei University of Engineering, Handan 056029, China. givesunny@163.com
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Liu
authors.forename: Xueling
authors.initials: X
authors.lastname: Wang
authors.forename: Zhenhong
authors.initials: Z
authors.lastname: Li
authors.forename: Yumin
authors.initials: Y
authors.lastname: Chen
authors.forename: Jianhua
authors.initials: J
authors.lastname: Chen
mesh_terms: D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005434:Flow Cytometry; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects* / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*
chemical_list: D004365:Drugs, Chinese Herbal