Insight into the mechanism of human herpesvirus 7 U21-mediated diversion of class I MHC molecules to lysosomes.
The U21 open reading frame from human herpesvirus-7 encodes a membrane protein that associates with and redirects class I MHC molecules to the lysosomal compartment. The mechanism by which U21 accomplishes this trafficking excursion is unknown. Here we have examined the contribution of localization, glycosylation, domain structure, and the absence of substrate class I MHC molecules on the ability of U21 to traffic to lysosomes. Our results suggest the existence of a cellular protein necessary for U21-mediated rerouting of class I MHC molecules.
Glosson
Nicole L
NL
Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA.
Gonyo
Patrick
P
May
Nathan A
NA
Schneider
Christine L
CL
Ristow
Laura C
LC
Wang
Qiuhong
Q
Hudson
Amy W
AW
D015153:Blotting, Western; D002352:Carrier Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D002454:Cell Differentiation; D003593:Cytoplasm / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000378:metabolism; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D005909:Glioblastoma / Q000378:metabolism*; D006031:Glycosylation; D015789:HLA-A2 Antigen / Q000235:genetics / Q000378:metabolism*; D016199:Herpesvirus 7, Human / Q000378:metabolism*; D006801:Humans; D047468:Immunoprecipitation; D008247:Lysosomes / Q000378:metabolism*; D010446:Peptide Fragments / Q000378:metabolism; D021381:Protein Transport; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured; D014764:Viral Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D001613:beta 2-Microglobulin / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism
D002352:Carrier Proteins; D015789:HLA-A2 Antigen; D010446:Peptide Fragments; D012333:RNA, Messenger; D034741:RNA, Small Interfering; C488324:U21 glycoprotein, human herpesvirus 7; D014764:Viral Proteins; D001613:beta 2-Microglobulin