Characterization of ionic currents and electrophysiological properties of goldfish somatotropes in primary culture.
Growth hormone release in goldfish is partly dependent on voltage-sensitive Ca(2+) channels but somatotrope electrophysiological events affecting such channel activities have not been elucidated in this system. The electrophysiological properties of goldfish somatotropes in primary culture were studied using the whole-cell and amphotericin B-perforated patch-clamp techniques. Intracellular Ca(2+) concentration ([Ca(2+)]i) of identified somatotropes was measured using Fura-2/AM dye. Goldfish somatotropes had an average resting membrane potential of -78.4 ± 4.6 mV and membrane input resistance of 6.2 ± 0.2 GΩ. Voltage steps from a holding potential of -90 mV elicited a non-inactivating outward current and transient inward currents at potentials more positive than 0 and -30 mV, respectively. Isolated current recordings indicate the presence of 4-aminopyridine- and tetraethylammonium (TEA)-sensitive K(+), tetrodotoxin (TTX)-sensitive Na(+), and nifedipine (L-type)- and ω-conotoxin GVIA (N-type)-sensitive Ca(2+) channels. Goldfish somatotropes rarely fire action potentials (APs) spontaneously, but single APs can be induced at the start of a depolarizing current step; this single AP was abolished by TTX and significantly reduced by nifedipine and ω-conotoxin GVIA. TEA increased AP duration and triggered repetitive AP firing resulting in an increase in [Ca(2+)]i, whereas TTX, nifedipine and ω-conotoxin GVIA inhibited TEA-induced [Ca(2+)]i pulses. These results indicate that in goldfish somatotropes, TEA-sensitive K(+) channels regulate excitability while TTX-sensitive Na(+) channels together with N- and L-type Ca channels mediates the depolarization phase of APs. Opening of voltage-sensitive Ca(2+) channels during AP firing leads to increases in [Ca(2+)]i.
Yu
Yi
Y
Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada.
Ali
Declan W
DW
Chang
John P
JP
D015761:4-Aminopyridine / Q000494:pharmacology; D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D001464:Barium / Q000502:physiology; D015220:Calcium Channels / Q000502:physiology; D002478:Cells, Cultured; D006054:Goldfish / Q000502:physiology*; D008564:Membrane Potentials / Q000502:physiology; D009543:Nifedipine; D018408:Patch-Clamp Techniques; D015221:Potassium Channels / Q000502:physiology; D015222:Sodium Channels / Q000502:physiology; D052683:Somatotrophs / Q000502:physiology*; D013757:Tetraethylammonium Compounds / Q000494:pharmacology; D020866:omega-Conotoxin GVIA / Q000494:pharmacology
D015220:Calcium Channels; D015221:Potassium Channels; D015222:Sodium Channels; D013757:Tetraethylammonium Compounds; D001464:Barium; D020866:omega-Conotoxin GVIA; D015761:4-Aminopyridine; D009543:Nifedipine