Promotion of cutaneous wound healing by local application of mesenchymal stem cells derived from human umbilical cord blood.
This study aimed to determine whether mesenchymal stem cells (MSCs) derived from umbilical cord blood (UCB) would promote cutaneous wound healing. MSCs from human UCB were isolated and identified. The characteristics of the isolated MSCs' growth and proliferation were assayed in vitro. The MSCs labeled with 5-bromodeoxyuridine (BrdU) were applied on fresh cutaneous mice wounds. The healing rates were surveyed. The distribution and the differentiation into keratinocytes of the labeled MSCs in the wound tissue were checked by immunohistochemistry staining. The isolated MSCs could grow and proliferate well in vitro. The isolated MSCs from UCB could be labeled by 5-bromodeoxyuridine successfully. The MSCs derived from UCB could enhance the healing of mice skin defect wounds, and it was found that the implanted MSCs could differentiate into keratinocyte in the wound tissue. It was demonstrated that MSCs from UCB can be isolated and proliferated successfully. The local administration of MSCs derived from UCB improves skin defect wound healing in mice.
Luo
Gaoxing
G
State Key Laboratory of Trauma, Burn and Combined Injury, Institute of Burn Research, Southwestern Hospital, Third Military Medical University, Chongqing, China. logxw@hotmail.com
Cheng
Wenguang
W
He
Weifeng
W
Wang
Xiaojuan
X
Tan
Jiangling
J
Fitzgerald
Mark
M
Li
Xianchang
X
Wu
Jun
J
D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D036101:Cord Blood Stem Cell Transplantation / Q000379:methods*; D004195:Disease Models, Animal; D004817:Epidermis / Q000473:pathology; D005260:Female; D005434:Flow Cytometry; D005500:Follow-Up Studies; D006801:Humans; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D051379:Mice; D008807:Mice, Inbred BALB C; D012867:Skin / Q000293:injuries* / Q000473:pathology; D016896:Treatment Outcome; D014945:Wound Healing; D014947:Wounds and Injuries / Q000473:pathology / Q000601:surgery*