title: Proteome analysis of pod and seed development in the model legume Lotus japonicus.
abstract: Legume pods serve important functions during seed development and are themselves sources of food and feed. Compared to seeds, the metabolism and development of pods are not well-defined. The present characterization of pods from the model legume Lotus japonicus, together with the detailed analyses of the pod and seed proteomes in five developmental stages, paves the way for comparative pathway analysis and provides new metabolic information. Proteins were analyzed by two-dimensional gel electrophoresis and tandem-mass spectrometry. These analyses lead to the identification of 604 pod proteins and 965 seed proteins, including 263 proteins distinguishing the pod. The complete data set is publicly available at http://www.cbs.dtu.dk/cgi-bin/lotus/db.cgi , where spots in a reference map are linked to experimental data, such as matched peptides, quantification values, and gene accessions. Identified pod proteins represented enzymes from 85 different metabolic pathways, including storage globulins and a late embryogenesis abundant protein. In contrast to seed maturation, pod maturation was associated with decreasing total protein content, especially proteins involved in protein biosynthesis and photosynthesis. Proteins detected only in pods included three enzymes participating in the urea cycle and four in nitrogen and amino group metabolism, highlighting the importance of nitrogen metabolism during pod development. Additionally, five legume seed proteins previously unassigned in the glutamate metabolism pathway were identified.
authors.affiliation: Department of Molecular Biology, University of Aarhus, DK-8000 Aarhus C, Denmark.
authors.forename: Gitte
authors.initials: G
authors.lastname: Nautrup-Pedersen
authors.forename: Svend
authors.initials: S
authors.lastname: Dam
authors.forename: Brian S
authors.initials: BS
authors.lastname: Laursen
authors.forename: Astrid L
authors.initials: AL
authors.lastname: Siegumfeldt
authors.forename: Kasper
authors.initials: K
authors.lastname: Nielsen
authors.forename: Nicolas
authors.initials: N
authors.lastname: Goffard
authors.forename: Hans Henrik
authors.initials: HH
authors.lastname: Stærfeldt
authors.forename: Carsten
authors.initials: C
authors.lastname: Friis
authors.forename: Shusei
authors.initials: S
authors.lastname: Sato
authors.forename: Satoshi
authors.initials: S
authors.lastname: Tabata
authors.forename: Andrea
authors.initials: A
authors.lastname: Lorentzen
authors.forename: Peter
authors.initials: P
authors.lastname: Roepstorff
authors.forename: Jens
authors.initials: J
authors.lastname: Stougaard
mesh_terms: D007887:Fabaceae; D005638:Fruit / Q000737:chemistry* / Q000254:growth & development; D000070116:Lotus / Q000737:chemistry* / Q000254:growth & development; D053858:Metabolic Networks and Pathways; D010940:Plant Proteins / Q000032:analysis*; D020543:Proteome / Q000032:analysis*; D012639:Seeds / Q000737:chemistry* / Q000254:growth & development; D053719:Tandem Mass Spectrometry
chemical_list: D010940:Plant Proteins; D020543:Proteome