Approaches and applications of quantitative LC-MS for proteomics and activitomics.
LC-MS is a powerful technique in biomolecular research. In addition to its uses as a tool for protein and peptide quantization, LC-MS can also be used to quantify the activity of signalling and metabolic pathways in a multiplex and comprehensive manner, i.e. as an 'activitomic' tool. Taking cancer research as an illustrative example of application, this review discusses the concepts of biochemical pathway analysis using LC-MS-based proteomic and activitomic techniques.
Cutillas
Pedro R
PR
Analytical Signalling Group, Centre for Cell Signalling, Institute of Cancer, Bart's and the London School of Medicine, Queen Mary University of London, London, UK.
Timms
John F
JF
D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D050356:Lipid Metabolism; D013058:Mass Spectrometry / Q000379:methods*; D055432:Metabolomics; D010750:Phosphoproteins / Q000032:analysis / Q000378:metabolism; D040901:Proteomics / Q000379:methods*
D010750:Phosphoproteins