title: Elevation of vascular endothelial growth factor in Indonesian advanced stage nasopharyngeal carcinoma.
abstract: Studies on the role of vascular endothelial growth factor (VEGF) as the most potent angiogenic factor in disease progression of nasopharyngeal carcinoma (NPC) remain limited, partly be due to the geographical distribution of the disease. However, it has never been reported from Indonesian population despite its common incidence. Therefore, we aimed to study the possible prognostic value of VEGF in Indonesian advanced stage NPC. A clinical examination, CT scan, and the tumor tissue and plasma collection were performed before a combined therapy, and a local control rate was reassessed every 3 months to determine progression-free survival (PFS). Plasma VEGF-A was measured in 40 patients by ELISA, and VEGF and vWF expressions were examined in 30 patients by immunohistochemistry. Survival curves were plotted based on plasma VEGF-A level, VEGF, and microvessel density (MVD) count indicated by vWF expressions vs PFS. The median follow up was 16 months. Patients with high VEGF-A level (≥ 834pg/ml) presented shorter survival rate, as compared to those of low level (< 834pg/ml) (41.2% vs 82.6%; p=0.009, log rank 6.81). Patients with overexpressed VEGF (≥ 25%) showed shorter survival than those with low expression (<25%) (45.5% vs 79.6%; p=0.05, log rank 3.84). Patients with higher MVD count (≥ 25%) also had shorter survival than those with lower MVD count (53.4% vs 80%; p=0.101, log rank=2.70). In conclusion, an elevated plasma VEGF level predicts shorter survival for Indonesian advanced stage NPC in this study. However, more samples may be required to draw a better conclusion on a possible role of VEGF as a prognostic factor in the disease progression of NPC. This data is also substantial for the development of anti-VEGF as a possible targeted therapy for NPC.
authors.affiliation: Sub-Department of Hematology-Oncology, Department of Internal Medicine, Faculty of Medicine, Gadjah Mada University, Yogyakarta, Indonesia.
authors.forename: Johan
authors.initials: J
authors.lastname: Kurnianda
authors.forename: Mardiah Suci
authors.initials: MS
authors.lastname: Hardianti
authors.lastname: Harijadi
authors.forename: Kartika Widayati
authors.initials: KW
authors.lastname: Taroeno-Hariadi
authors.forename: Ibnu
authors.initials: I
authors.lastname: Purwanto
authors.forename: Sofia Mubarika
authors.initials: SM
authors.lastname: Haryana
authors.forename: Maesadjie Salugu
authors.initials: MS
authors.lastname: Tjokronagoro
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009303:Nasopharyngeal Neoplasms / Q000098:blood supply* / Q000401:mortality / Q000473:pathology; D009367:Neoplasm Staging; D015996:Survival Rate; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis*; D014841:von Willebrand Factor / Q000032:analysis
chemical_list: D042461:Vascular Endothelial Growth Factor A; D014841:von Willebrand Factor