PCR diagnostics and monitoring of adenoviral infections in hematopoietic stem cell transplantation recipients.
After stem cell transplantation, human patients are prone to life-threatening opportunistic infections with a plethora of microorganisms. We report a retrospective study on 116 patients (98 children, 18 adults) who were transplanted in a pediatric bone marrow transplantation unit. Blood, urine and stool samples were collected and monitored for adenovirus (AdV) DNA using polymerase chain reaction (PCR) and real-time PCR (RT-PCR) on a regular basis. AdV DNA was detected in 52 (44.8%) patients, with mortality reaching 19% in this subgroup. Variables associated with adenovirus infection were transplantations from matched unrelated donors and older age of the recipient. An increased seasonal occurrence of adenoviral infections was observed in autumn and winter. Analysis of immune reconstitution showed a higher incidence of AdV infections during periods of low T-lymphocyte count. This study also showed a strong interaction between co-infections of AdV and BK polyomavirus in patients undergoing hematopoietic stem cell transplantations.
Bil-Lula
Iwona
I
Department of Clinical Chemistry, University of Medicine in Wrocław, ul. Pasteura 2, 50-367 Wrocław, Poland. iwonabil@anmed.am.wroc.pl
Ussowicz
Marek
M
Rybka
Blanka
B
Wendycz-Domalewska
Danuta
D
Ryczan
Renata
R
Gorczyńska
Ewa
E
Kałwak
Krzysztof
K
Woźniak
Mieczysław
M
D000257:Adenoviridae Infections / Q000175:diagnosis* / Q000453:epidemiology* / Q000401:mortality / Q000821:virology; D000260:Adenoviruses, Human / Q000302:isolation & purification*; D000293:Adolescent; D000328:Adult; D001769:Blood / Q000821:virology; D002648:Child; D002675:Child, Preschool; D005243:Feces / Q000821:virology; D005260:Female; D018380:Hematopoietic Stem Cell Transplantation / Q000009:adverse effects*; D006801:Humans; D007223:Infant; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods; D015995:Prevalence; D012189:Retrospective Studies; D012307:Risk Factors; D014556:Urine / Q000821:virology; D014773:Virology / Q000379:methods