Changes in the two-dimensional electrophoresis pattern of the Parkinson's disease related protein DJ-1 in human SH-SY5Y neuroblastoma cells after dopamine treatment.
DJ-1 is a mitochondrial protein linked to Parkinson's disease. DJ-1 has been suggested to have several possible functions, although it has been mainly associated to oxidative stress defence. Changes in the two-dimensional electrophoresis pattern have been thoroughly described as a consequence of oxidative modification of the Cys106 residue. There is accumulating evidence supporting a specific role of DJ-1 in protecting dopaminergic neurons from dopamine itself. By exposing SH-SY5Y human neuroblastoma catecholaminergic cells to dopamine, we observed a specific increase in the most acidic forms in the DJ-1 two-dimensional electrophoresis pattern together with a significant decrease of the most basic spot. Unlike cells exposed to generic oxidative conditions, no additional shift was observed. The results are corroborated by a meta-analysis of the literature showing that in the absence of dopamine treatment the specific acidic form is underrepresented.
Alberio
Tiziana
T
Department of Structural and Functional Biology, Center of Neuroscience, University of Insubria, Busto Arsizio, VA, Italy.
Colapinto
Monica
M
Natale
Massimo
M
Ravizza
Raffaella
R
Gariboldi
Marzia B
MB
Bucci
Enrico M
EM
Lopiano
Leonardo
L
Fasano
Mauro
M
D015153:Blotting, Western; D045744:Cell Line, Tumor; D004298:Dopamine / Q000494:pharmacology*; D015259:Dopamine Agents / Q000494:pharmacology; D015180:Electrophoresis, Gel, Two-Dimensional; D005434:Flow Cytometry; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism*; D009447:Neuroblastoma / Q000378:metabolism*; D015513:Oncogene Proteins / Q000378:metabolism*; D010300:Parkinson Disease; D000071617:Protein Deglycase DJ-1; D020033:Protein Isoforms / Q000187:drug effects / Q000378:metabolism
D015259:Dopamine Agents; D047908:Intracellular Signaling Peptides and Proteins; D015513:Oncogene Proteins; D020033:Protein Isoforms; C105131:PARK7 protein, human; D000071617:Protein Deglycase DJ-1; D004298:Dopamine