Utilization of deoiled Jatropha curcas seed cake for production of xylanase from thermophilic Scytalidium thermophilum.
Jatropha curcas is a major biodiesel crop. Large amount of deoiled cake is generated as by-product during biodiesel production from its seeds. Deoiled J. curcas seed cake was assessed as substrate for the production of xylanase from thermophilic fungus Scytalidium thermophilum by solid-state fermentation. The seed cake was efficiently utilized by S. thermophilum for its growth during which it produced good amount of heat stable extracellular xylanase. The solid-state fermentation conditions were optimized for maximum xylanase production. Under the optimized conditions viz. deoiled seed cake supplemented with 1% oat-spelt xylan, adjusted to pH 9.0, moisture content 1:3 w/v, inoculated with 1×10(6) spores per 5 g cake and incubated at 45 °C, 1455 U xylanase/g deoiled seed cake was obtained. The xylanase was useful in biobleaching of paper pulp. Solid-state fermentation of deoiled cake appears a potentially viable approach for its effective utilization.
Joshi
Chetna
C
Enzyme and Microbial Biochemistry Lab, Department of Chemistry, Indian Institute of Technology, Hauz Khas, New Delhi 110 016, India.
Khare
S K
SK
D001203:Ascomycota / Q000187:drug effects / Q000201:enzymology*; D002244:Carbon / Q000494:pharmacology; D002482:Cellulose / Q000737:chemistry; D043364:Endo-1,4-beta Xylanases / Q000096:biosynthesis*; D005285:Fermentation / Q000187:drug effects; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D031286:Jatropha / Q000737:chemistry* / Q000187:drug effects; D009584:Nitrogen / Q000494:pharmacology; D010209:Paper; D010938:Plant Oils / Q000302:isolation & purification*; D012639:Seeds / Q000737:chemistry* / Q000187:drug effects; D013696:Temperature; D014866:Waste Products / Q000032:analysis*; D014867:Water
D010938:Plant Oils; D014866:Waste Products; D014867:Water; D002244:Carbon; D002482:Cellulose; C027433:bagasse; D043364:Endo-1,4-beta Xylanases; D009584:Nitrogen