Mesoporous platinum electrodes for amperometric determination of sugars with anion exchange chromatography.
A simple amperometric detection (AD) method based on mesoporous platinum (Pt) electrodes has been developed for the determination of sugars (glucose, fructose, and sucrose) with high-performance anion-exchange chromatography (HPAEC). The amperometric detection is based on the direct oxidation of sugars on mesoporous Pt films formed on a gold electrode. The mesoporous Pt electrode (roughness factor of 243) sensitively responded to glucose, fructose, and sucrose in 80 mM sodium hydroxide solution as an alkaline mobile-phase for HPAEC. Under the optimum conditions, the limits of detection (S/N = 3) in these sugars were 0.24, 0.29, and 1.8 mM, for glucose, fructose, and sucrose, respectively. The reproducibility (relative standard deviation) of the measurements was less than 3.5%. The present method was applied to the determination of sugars in apple juice. The recoveries for all sugars ranged from 97 to 99%.
Han
Jee Hoon
JH
Department of Chemistry, Yonsei University, Seoul 120-749, Korea.
Choi
Han Nim
HN
Park
Sejin
S
Chung
Taek Dong
TD
Lee
Won-Yong
WY
D000838:Anions / Q000032:analysis; D002138:Calibration; D002241:Carbohydrates / Q000032:analysis* / Q000737:chemistry; D002852:Chromatography, Ion Exchange; D004566:Electrodes; D005632:Fructose / Q000032:analysis; D005947:Glucose / Q000032:analysis; D006046:Gold; D027845:Malus / Q000378:metabolism; D010984:Platinum; D015203:Reproducibility of Results; D013395:Sucrose / Q000032:analysis
D000838:Anions; D002241:Carbohydrates; D005632:Fructose; D010984:Platinum; D013395:Sucrose; D006046:Gold; D005947:Glucose