title: Protein and lipid oxidative damage in streptozotocin-induced diabetic rats submitted to forced swimming test: the insulin and clonazepam effect.
abstract: Diabetes may modify central nervous system functions and is associated with moderate cognitive deficits and changes in the brain, a condition that may be referred to as diabetic encephalopathy. The prevalence of depression in diabetic patients is higher than in the general population, and clonazepam is being used to treat this complication. Oxidative stress may play a role in the development of diabetes complications. We investigated oxidative stress parameters in streptozotocin-induced diabetic rats submitted to forced swimming test (STZ) and evaluated the effect of insulin (STZ-INS) and/or clonazepam (STZ-CNZ and STZ-INS-CNZ) acute treatment on these animal model. Oxidative damage to proteins measured as carbonyl content in plasma was significantly increased in STZ group compared to STZ treated groups. Malondialdehyde plasma levels were significantly reduced in STZ-INS and STZ-INS-CNZ groups when compared to STZ rats, being significantly reduced in STZ-INS-CNZ than STZ-INS rats. The activities of the antioxidant enzymes catalase, superoxide dismutase and glutathione peroxidase showed no significant differences among all groups of animals. These findings showed that protein and lipid damage occurs in this diabetes/depression animal model and that the associated treatment of insulin and clonazepam is capable to protect against oxidative damage in this experimental model.
authors.affiliation: Programa de Pós-Graduação em Ciências Farmacêuticas, Porto Alegre, RS, Brazil. manowayhs@yahoo.com.br
authors.forename: Carlos Alberto Yasin
authors.initials: CA
authors.lastname: Wayhs
authors.forename: Vanusa
authors.initials: V
authors.lastname: Manfredini
authors.forename: Angela
authors.initials: A
authors.lastname: Sitta
authors.forename: Marion
authors.initials: M
authors.lastname: Deon
authors.forename: Graziela
authors.initials: G
authors.lastname: Ribas
authors.forename: Camila
authors.initials: C
authors.lastname: Vanzin
authors.forename: Giovana
authors.initials: G
authors.lastname: Biancini
authors.forename: Marcelo
authors.initials: M
authors.lastname: Ferri
authors.forename: Maurício
authors.initials: M
authors.lastname: Nin
authors.forename: Helena Maria Tannhauser
authors.initials: HM
authors.lastname: Barros
authors.forename: Carmen Regla
authors.initials: CR
authors.lastname: Vargas
mesh_terms: D000818:Animals; D002998:Clonazepam / Q000494:pharmacology* / Q000627:therapeutic use; D003866:Depressive Disorder / Q000188:drug therapy* / Q000209:etiology / Q000378:metabolism*; D048909:Diabetes Complications / Q000378:metabolism*; D004195:Disease Models, Animal; D018757:GABA Modulators / Q000494:pharmacology / Q000627:therapeutic use; D007004:Hypoglycemic Agents / Q000378:metabolism / Q000494:pharmacology / Q000627:therapeutic use; D007328:Insulin / Q000494:pharmacology* / Q000627:therapeutic use; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D013315:Stress, Psychological / Q000150:complications / Q000378:metabolism; D013550:Swimming / Q000523:psychology
chemical_list: D018757:GABA Modulators; D007004:Hypoglycemic Agents; D007328:Insulin; D002998:Clonazepam