title: Podoplanin-expressing cells derived from bone marrow play a crucial role in postnatal lymphatic neovascularization.
abstract: BACKGROUND
  Emerging evidence has suggested a contribution of bone marrow (BM) cells to lymphatic vessel formation; however, the exact phenotype of the cells with lymphatic endothelial progenitor cell function has yet to be identified. Here, we investigate the identity of BM-derived lymphatic endothelial progenitor cells and their role in lymphatic neovascularization.
  
  
  RESULTS
  Culture of BM-mononuclear cells in the presence of vascular endothelial growth factors A and C and endothelial growth factor resulted in expression of lymphatic endothelial cell markers. Among these cells, podoplanin(+) cells were isolated by magnetic-activated cell sorting and characterized by fluorescence-activated cell sorter analysis and immunocytochemistry. These podoplanin(+) cells highly express markers for lymphatic endothelial cells, hematopoietic lineages, and stem/progenitor cells; on further cultivation, they generate lymphatic endothelial cells. We further confirmed that podoplanin(+) cells exist in small numbers in BM and peripheral blood of normal mice but are significantly (15-fold) augmented on lymphangiogenic stimuli such as tumor implantation. Next, to evaluate the potential of podoplanin(+) cells for the formation of new lymphatic vessels in vivo, we injected culture-isolated or freshly isolated BM-derived podoplanin(+) cells into wound and tumor models. Immunohistochemistry demonstrated that the injected cells were incorporated into the lymphatic vasculature, displayed lymphatic endothelial cell phenotypes, and increased lymphatic vascular density in tissues, suggesting lymphvasculogenesis. Podoplanin(+) cells also expressed high levels of lymphangiogenic cytokines and increased proliferation of lymphatic endothelial cells during coculture, suggesting a lymphangiogenic or paracrine role.
  
  
  CONCLUSIONS
  Our results provide compelling evidence that BM-derived podoplanin(+) cells, a previously unrecognized cell type, function as lymphatic endothelial progenitor cells and participate in postnatal lymphatic neovascularization through both lymphvasculogenesis and lymphangiogenesis.
authors.affiliation: Division of Cardiology, Department of Medicine, Emory University School of Medicine, 1639 Pierce Drive, Atlanta, GA 30322, USA. yyoon5@emory.edu
authors.forename: Ji Yoon
authors.initials: JY
authors.lastname: Lee
authors.forename: Changwon
authors.initials: C
authors.lastname: Park
authors.forename: Yong Pil
authors.initials: YP
authors.lastname: Cho
authors.forename: Eugine
authors.initials: E
authors.lastname: Lee
authors.forename: Hyongbum
authors.initials: H
authors.lastname: Kim
authors.forename: Pilhan
authors.initials: P
authors.lastname: Kim
authors.forename: Seok H
authors.initials: SH
authors.lastname: Yun
authors.forename: Young-sup
authors.initials: YS
authors.lastname: Yoon
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000502:physiology; D018929:Cell Culture Techniques; D002455:Cell Division; D003315:Cornea / Q000166:cytology; D042783:Endothelial Cells / Q000166:cytology; D004815:Epidermal Growth Factor / Q000235:genetics; D006410:Hematopoiesis; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology; D042583:Lymphangiogenesis; D042601:Lymphatic Vessels / Q000166:cytology / Q000473:pathology*; D008562:Membrane Glycoproteins / Q000235:genetics*; D051379:Mice; D009389:Neovascularization, Pathologic / Q000473:pathology*; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics; D000098853:Podoplanin
chemical_list: D004815:Epidermal Growth Factor; D008562:Membrane Glycoproteins; D042461:Vascular Endothelial Growth Factor A; C490441:Pdpn protein, mouse; D000098853:Podoplanin