title: Electrochemical study of spiramycin and its determination in pharmaceutical preparation.
abstract: Spiramycin (SPY) is a medium-spectrum antibiotic with high effectiveness against Gram-positive bacteria. The voltammetric behaviour of spiramycin was studied using differential pulse polarography (DPP) and square wave polarography (SWP). The drug in Britton-Robinson buffer (pH 11.5) is reduced at - 1.45 V, giving rise to a well-defined cathodic peak using hanging mercury drop electrode (HMDE) versus Ag/AgCl electrode. This peak is attributed to the reduction of the aldehyde group. The results proved that the reduction of SPY is an irreversible diffusion-controlled process. The diffusion current-concentration relationship was shown to be rectilinear over the range of 20-80 and 0.8-80 µg ml(-1) using DPP and SWP modes, respectively, with detection limit of 8.5 µg ml(-1) (1.01 × 10(-5) M) and 0.46 µg ml(-1) (5.46 × 10(-7) M) for DPP and SWP modes, respectively. A mechanism is postulated for the reduction of SPY. The proposed techniques were successfully applied to the determination of the studied compound either in pure form or in its formulation.
authors.affiliation: Faculty of Pharmacy, Department of Pharmaceutical Analytical Chemistry, University of Alexandria, El-Messalah, Alexandria 21521, Egypt. rmmy1973@yahoo.com
authors.forename: Rasha M
authors.initials: RM
authors.lastname: Youssef
authors.forename: Hadir M
authors.initials: HM
authors.lastname: Maher
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000737:chemistry; D004563:Electrochemistry; D004566:Electrodes; D008628:Mercury / Q000737:chemistry; D011048:Polarography / Q000379:methods*; D018030:Silver Compounds / Q000737:chemistry; D015572:Spiramycin / Q000032:analysis* / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D018030:Silver Compounds; D015572:Spiramycin; D008628:Mercury; C037548:silver chloride