title: Whole body hypothermia and oxidative stress in babies with hypoxic-ischemic brain injury.
abstract: According to increasing evidence, hypothermia can significantly improve outcomes in term neonates manifesting asphyxic insult and hypoxic-ischemic encephalopathy. Oxidative stress plays a key role in hypoxic-ischemic and inflammatory brain injuries. We investigated the impact of hypothermia on oxidative stress in babies with hypoxic-ischemic encephalopathy. Term infants were randomly selected for treatment with moderate whole body hypothermia or standard care on normothermia, after perinatal asphyxia. Total hydroperoxides as biochemical markers of oxidative stress, and C-reactive protein as a marker of inflammation, were assayed in blood samples drown at 6, 12, 24, 48, and 72 postnatal hours. In both hypothermic and normothermic groups, total hydroperoxides and C-reactive protein exhibited a continuous increase in the first days after birth. Nevertheless, a tendency was evident for slower and smaller elevations of total hydroperoxides and C-reactive protein in hypothermic compared with normothermic infants. A significant correlation was observed between total hydroperoxides and C-reactive protein in all patients, indicating an association between inflammation and oxidative stress during asphyxia. The slower increase and lower peaks of total hydroperoxides in the hypothermic group support the hypothesis that postasphyxic oxidative stress may be reduced by hypothermia.
authors.affiliation: Department of Pediatrics, Obstetrics, and Reproductive Medicine, University of Siena, Siena, Italy.
authors.forename: Serafina
authors.initials: S
authors.lastname: Perrone
authors.forename: Miklós
authors.initials: M
authors.lastname: Szabó
authors.forename: Carlo Valerio
authors.initials: CV
authors.lastname: Bellieni
authors.forename: Mariangela
authors.initials: M
authors.lastname: Longini
authors.forename: Márta
authors.initials: M
authors.lastname: Bangó
authors.forename: Dorottya
authors.initials: D
authors.lastname: Kelen
authors.forename: András
authors.initials: A
authors.lastname: Treszl
authors.forename: Simona
authors.initials: S
authors.lastname: Negro
authors.forename: Maria Luisa
authors.initials: ML
authors.lastname: Tataranno
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Buonocore
mesh_terms: D002097:C-Reactive Protein / Q000378:metabolism; D005260:Female; D006801:Humans; D007036:Hypothermia, Induced; D020925:Hypoxia-Ischemia, Brain / Q000378:metabolism / Q000628:therapy*; D007231:Infant, Newborn; D007249:Inflammation / Q000378:metabolism / Q000628:therapy; D008297:Male; D018384:Oxidative Stress; D018709:Statistics, Nonparametric; D016896:Treatment Outcome
chemical_list: D002097:C-Reactive Protein