title: The role of hyaluronic acid binding assay in choosing the fertilization method for patients undergoing IVF for unexplained infertility.
abstract: OBJECTIVE
  Patients with unexplained infertility may have fertilization problems. Split fertilization (ICSI and conventional IVF on sibling oocytes) is often used to avoid poor fertilization. Our aim was to assess the ability of hyaluronic acid binding (HA-binding) assay to predict spontaneous fertilization during IVF.
  
  
  METHODS
  Prospective, blinded, controlled trial. Patients undergoing their first IVF cycle for unexplained infertility were eligible. Split fertilization was used. IVF and ICSI fertilization rates and embryo development based on 3 HA-binding cut-offs (< 60%; 60-80%; >80%) were compared.
  
  
  RESULTS
  ICSI fertilization was higher than IVF, but none of the HA-binding cut-off levels predicted those cases where IVF was less effective, therefore ICSI only would have lead to improved outcome. Embryo development and morphology were similar in all cut-off groups.
  
  
  CONCLUSIONS
  HA-binding did not predict spontaneous fertilization in patients with unexplained infertility undergoing IVF treatment. When it was used for "screening" it did not help to select the method of fertilization.
authors.affiliation: IVF Center, Kaali Institute, Budapest, Hungary. peterkovacs1970@hotmail.com
authors.forename: Peter
authors.initials: P
authors.lastname: Kovacs
authors.forename: Timea
authors.initials: T
authors.lastname: Kovats
authors.forename: Attila
authors.initials: A
authors.lastname: Sajgo
authors.forename: Janos
authors.initials: J
authors.lastname: Szollosi
authors.forename: Szabolcs
authors.initials: S
authors.lastname: Matyas
authors.forename: Steven G
authors.initials: SG
authors.lastname: Kaali
mesh_terms: D000328:Adult; D005260:Female; D005306:Fertilization; D005307:Fertilization in Vitro / Q000379:methods*; D006801:Humans; D006820:Hyaluronic Acid; D007248:Infertility, Male / Q000628:therapy; D008297:Male; D011247:Pregnancy; D018873:Pregnancy Rate; D011446:Prospective Studies; D016037:Single-Blind Method; D020554:Sperm Injections, Intracytoplasmic / Q000379:methods*; D013094:Spermatozoa / Q000378:metabolism
chemical_list: D006820:Hyaluronic Acid