Motile and non-motile sperm diagnostic manipulation using optoelectronic tweezers.
Optoelectronic tweezers was used to manipulate human spermatozoa to determine whether their response to OET predicts sperm viability among non-motile sperm. We review the electro-physical basis for how live and dead human spermatozoa respond to OET. The maximal velocity that non-motile spermatozoa could be induced to move by attraction or repulsion to a moving OET field was measured. Viable sperm are attracted to OET fields and can be induced to move at an average maximal velocity of 8.8 ± 4.2 µm s(-1), while non-viable sperm are repelled to OET, and are induced to move at an average maximal velocity of -0.8 ± 1.0 µm s(-1). Manipulation of the sperm using OET does not appear to result in increased DNA fragmentation, making this a potential method by which to identify viable non-motile sperm for assisted reproductive technologies.
Ohta
Aaron T
AT
Department of Electrical Engineering, University of Hawaii at Manoa, Honolulu, HI 96822, USA. aohta@hawaii.edu
Garcia
Maurice
M
Valley
Justin K
JK
Banie
Lia
L
Hsu
Hsan-Yin
HY
Jamshidi
Arash
A
Neale
Steven L
SL
Lue
Tom
T
Wu
Ming C
MC
D015925:Cryopreservation; D053938:DNA Fragmentation; D004867:Equipment Design; D006801:Humans; D008297:Male; D052898:Optical Tweezers; D027724:Reproductive Techniques, Assisted; D013076:Sperm Count; D020554:Sperm Injections, Intracytoplasmic / Q000379:methods; D013081:Sperm Motility; D053625:Sperm Retrieval; D013094:Spermatozoa / Q000473:pathology / Q000502:physiology