title: Uptake of gold nanoparticles in murine macrophage cells without cytotoxicity or production of pro-inflammatory mediators.
abstract: More information characterizing the biological responses to nanoparticles is needed to allow the U.S. Food and Drug Administration to evaluate the safety and effectiveness of products with nano-scale components. The potential cytotoxicity and inflammatory responses of Au NPs (60 nm, NIST standard reference materials) were investigated in murine macrophages. Cytotoxicity was evaluated by MTT and LDH assays. Cytokines (IL-6, TNF-α), nitric oxide, and ROS were assayed to assess inflammatory responses. Morphological appearance and localization of particles were examined by high resolution illumination microscopy, transmission electron microscopy (TEM), and scanning TEM coupled with EDX spectroscopy. Results showed no cytotoxicity and no elevated production of proinflammatory mediators; however, imaging analyses demonstrated cellular uptake of Au NPs and localization within intracellular vacuoles. These results suggest that 60 nm Au NPs, under the exposure conditions tested, are not cytotoxic, nor elicit pro-inflammatory responses. The localization of Au NPs in intracellular vacuoles suggests endosomal containment and an uptake mechanism involving endocytosis.
authors.affiliation: Center for Devices and Radiological Health, Food and Drug Administration, Silver Spring , MD 20993, USA.
authors.forename: Qin
authors.initials: Q
authors.lastname: Zhang
authors.forename: Victoria M
authors.initials: VM
authors.lastname: Hitchins
authors.forename: Amanda M
authors.initials: AM
authors.lastname: Schrand
authors.forename: Saber M
authors.initials: SM
authors.lastname: Hussain
authors.forename: Peter L
authors.initials: PL
authors.lastname: Goering
mesh_terms: D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D006046:Gold / Q000737:chemistry*; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism*; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000633:toxicity*; D051379:Mice
chemical_list: D006046:Gold