title: Isolation of aggresomes and other large aggregates.
abstract: Upon permanent stresses and in various diseases, small protein aggregates may accumulate in cells and cause toxicity. A recently discovered protective system transports these aggregates to the centrosome location via microtubules, to form a large agglomerate of aggregates called the aggresomes. Here, we describe a newly developed method for isolating aggresomes. This principle can also be used for purification of other large structures and even organelles.
authors.affiliation: Department of Biochemistry, Boston University School of Medicine, Boston, Massachusetts, USA.
authors.forename: Anatoli B
authors.initials: AB
authors.lastname: Meriin
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Michael Y
authors.initials: MY
authors.lastname: Sherman
mesh_terms: D000818:Animals; D001671:Biochemistry / Q000379:methods*; D018385:Centrosome / Q000378:metabolism*; D006801:Humans; D008869:Microtubule-Associated Proteins / Q000302:isolation & purification / Q000378:metabolism*; D046912:Multiprotein Complexes / Q000302:isolation & purification / Q000378:metabolism*; D055503:Protein Multimerization; D021381:Protein Transport
chemical_list: D008869:Microtubule-Associated Proteins; D046912:Multiprotein Complexes