Using light to control signaling cascades in live neurons.
Understanding the complexity of neuronal biology requires the manipulation of cellular processes with high specificity and spatio-temporal precision. The recent development of synthetic photo-activatable proteins designed using the light-oxygen-voltage and phytochrome domains provides a new set of tools for genetically targeted optical control of cell signaling. Their modular design, functional diversity, precisely controlled activity and in vivo applicability offer many advantages for investigating neuronal function. Although designing these proteins is still a considerable challenge, future advances in rational protein design and a deeper understanding of their photoactivation mechanisms will allow the development of the next generation of optogenetic techniques.
Rana
Anshul
A
Graduate Program in Biochemistry, Stanford University, Beckman Center B400, 279 Campus Drive, Stanford, CA 94305, USA.
Dolmetsch
Ricardo E
RE
D000818:Animals; D018390:Gene Targeting / Q000379:methods / Q000639:trends; D006801:Humans; D008164:Luminescent Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D009474:Neurons / Q000737:chemistry / Q000378:metabolism* / Q000502:physiology; D010777:Photochemistry / Q000379:methods* / Q000639:trends; D025604:Rhodopsins, Microbial / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology* / Q000528:radiation effects; D013194:Staining and Labeling / Q000379:methods*
D008164:Luminescent Proteins; D025604:Rhodopsins, Microbial