Measuring the stoichiometry of functional PspA complexes in living bacterial cells by single molecule photobleaching.
We report a general method based on wide-field fluorescence imaging of single molecule photobleaching and the Chung-Kennedy algorithm to measure the stoichiometry of functional protein complexes in living bacterial cells.
Lenn
Tchern
T
Division of Biology, Imperial College London, London, UK SW7 2AZ.
Gkekas
Christos N
CN
Bernard
Laurent
L
Engl
Christoph
C
Jovanovic
Goran
G
Buck
Martin
M
Ying
Liming
L
D000465:Algorithms; D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D004926:Escherichia coli / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry*; D006360:Heat-Shock Proteins / Q000737:chemistry* / Q000378:metabolism; D007089:Image Enhancement; D008856:Microscopy, Fluorescence; D038761:Photobleaching; D013237:Stereoisomerism
D001426:Bacterial Proteins; D005456:Fluorescent Dyes; D006360:Heat-Shock Proteins; C069816:phage shock protein, Bacteria