title: [Construction and identification of a vector inserted with complete genome of poliovirus strain Sabin I].
abstract: OBJECTIVE
  To develop a vector inserted with complete genome of poliovirus strain Sabin I.
  
  
  METHODS
  The 3 fragments of the complete genome of Sabin I was amplified and cloned to pEASY-T3 by molecular biological technology. These cloned pEASY-T3 were then digested by Restriction enzymes and ligated to pWSK29 step by step and identified.
  
  
  RESULTS
  The complete genome of poliovirus strain Sabin I was successfully cloned into vector pWSK29 with 9 nucleotide mutations.
  
  
  CONCLUSIONS
  The complete genome plasmid was constructed and it provided a basis for further research of the function of Sabin I.
authors.affiliation: College of Life sciences, Jilin University, Changchun 130012, China.
authors.forename: Hong-Hui
authors.initials: HH
authors.lastname: Shen
authors.forename: Jun
authors.initials: J
authors.lastname: Hou
authors.forename: Yan
authors.initials: Y
authors.lastname: Hu
authors.forename: Bing-Ke
authors.initials: BK
authors.lastname: Bai
authors.forename: Zhi-Jie
authors.initials: ZJ
authors.lastname: Wang
authors.forename: Xiang-Hui
authors.initials: XH
authors.lastname: Yu
authors.forename: Wei
authors.initials: W
authors.lastname: Kong
authors.forename: Ze
authors.initials: Z
authors.lastname: Liu
authors.forename: Pan-Yong
authors.initials: PY
authors.lastname: Mao
mesh_terms: D003001:Cloning, Molecular; D005822:Genetic Vectors / Q000235:genetics*; D016679:Genome, Viral; D009154:Mutation; D017955:Poliovirus / Q000235:genetics*