title: Alpha-1 antitrypsin variants in plasma from HIV-infected patients revealed by proteomic and glycoproteomic analysis.
abstract: Novel tools are necessary to explore proteins related to human immunodeficiency virus (HIV) infection. In this work, proteomic and glycoproteomic technology were employed to examine plasma samples from HIV-positive patients. Through comparative proteome analysis of normal and HIV-positive plasma samples, 19 differentially expressed protein spots related to 12 non-redundant proteins were identified by ESI-ion trap MS. Among these, the 130-kDa isoform of α-1-antitrypsin was found to be decreased in HIV-positive patients while another variant with a molecular weight of 40 kDa was increased. SWISS-2-D-PAGE reference gel and protein sequence comparisons of the 40-kDa protein showed homology with α-1-antitrypsin minus the N-terminus, and its identity was further confirmed by 1-D Western blotting and glycoproteomic analysis. In all, our results showed that proteomics and glycoproteomics are powerful tools for discovering proteins related to HIV infection. Furthermore, this 40-kDa variant of α-1-antitrypsin found in the plasma of HIV-positive individuals may prove to be a potentially useful biomarker for anti-HIV research according to bioinformatics analysis.
authors.affiliation: Shanghai Public Health Clinical Center affiliated to Fudan University, Shanghai, PR China.
authors.forename: Lijun
authors.initials: L
authors.lastname: Zhang
authors.forename: Xiaofang
authors.initials: X
authors.lastname: Jia
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Zhang
authors.forename: Jing
authors.initials: J
authors.lastname: Cao
authors.forename: Pengyuan
authors.initials: P
authors.lastname: Yang
authors.forename: Chao
authors.initials: C
authors.lastname: Qiu
authors.forename: Yinzhong
authors.initials: Y
authors.lastname: Shen
authors.forename: Fang
authors.initials: F
authors.lastname: Ma
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.forename: Jianjun
authors.initials: J
authors.lastname: Sun
authors.forename: Fang
authors.initials: F
authors.lastname: Shen
authors.forename: Lin
authors.initials: L
authors.lastname: Yin
authors.forename: Liwen
authors.initials: L
authors.lastname: Liu
authors.forename: Yamin
authors.initials: Y
authors.lastname: Yao
authors.forename: Hongzhou
authors.initials: H
authors.lastname: Lu
mesh_terms: D000328:Adult; D000595:Amino Acid Sequence; D015415:Biomarkers / Q000097:blood / Q000737:chemistry; D015153:Blotting, Western; D016022:Case-Control Studies; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006023:Glycoproteins / Q000097:blood* / Q000737:chemistry; D006031:Glycosylation; D015658:HIV Infections / Q000097:blood*; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D008970:Molecular Weight; D020033:Protein Isoforms; D040901:Proteomics / Q000379:methods*; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization; D000515:alpha 1-Antitrypsin / Q000097:blood* / Q000737:chemistry
chemical_list: D015415:Biomarkers; D006023:Glycoproteins; D020033:Protein Isoforms; D000515:alpha 1-Antitrypsin