Quantification of cystatin C in cerebrospinal fluid from various neurological disorders and correlation with G73A polymorphism in CST3.
Cystatin C (CC) is a cysteine protease inhibitor abundantly expressed in the central nervous system. Bunina bodies, small eosinophilic intraneuronal inclusions, are stain positive for CC and are the most specific histological hallmark of amyotrophic lateral sclerosis (ALS). In this study, employing a latex turbidimetric immunoassay, levels of CC in cerebrospinal fluid (CSF) were quantified in 130 age-matched individuals with either a neurological disorder [ALS, Alzheimer's disease (AD), Parkinson's disease (PD), tauopathy (TP), multiple system atrophy (MSA), chronic inflammatory demyelinating polyneuropathy (CIDP)] or no known neurological condition (normal control, NC). The CC level in CSF was found to be correlated with the age during the investigation but not the protein concentration. There was no difference in CC levels between NC and ALS or CIDP cases, whereas CC levels were significantly lower in MSA compared with NC. Of the 130 cases, 96 were genotyped, and G/A or A/A polymorphism at +73 within the CST3 gene was found in 28 individuals. The CC level was significantly lower in the combined group of G/A and A/A genotypes compared with G/G. The present data demonstrate that the level of CC in CSF should not be considered as a biomarker of ALS, but there is a correlation between CC levels and the CST3 genotype.
Yamamoto-Watanabe
Yukiko
Y
Department of Neurology, Hirosaki University Graduate School of Medicine, Hirosaki, Aomori 036-8562, Japan. miwata36@cc.hirosaki-u.ac.jp
Watanabe
Mitsunori
M
Jackson
Mandy
M
Akimoto
Hiroyuki
H
Sugimoto
Kazuhiro
K
Yasujima
Minoru
M
Wakasaya
Yasuhito
Y
Matsubara
Etsuro
E
Kawarabayashi
Takeshi
T
Harigaya
Yasuo
Y
Lyndon
Alastair R
AR
Shoji
Mikio
M
D000368:Aged; D000369:Aged, 80 and over; D000409:Alanine; D000544:Alzheimer Disease / Q000134:cerebrospinal fluid / Q000235:genetics; D000690:Amyotrophic Lateral Sclerosis / Q000134:cerebrospinal fluid / Q000235:genetics; D015415:Biomarkers; D016022:Case-Control Studies; D055316:Cystatin C / Q000097:blood / Q000134:cerebrospinal fluid* / Q000235:genetics; D005260:Female; D005998:Glycine; D006801:Humans; D007118:Immunoassay / Q000379:methods; D007840:Latex; D008297:Male; D008875:Middle Aged; D019578:Multiple System Atrophy / Q000134:cerebrospinal fluid / Q000235:genetics; D009391:Nephelometry and Turbidimetry / Q000379:methods; D009422:Nervous System Diseases / Q000097:blood / Q000134:cerebrospinal fluid* / Q000235:genetics*; D010300:Parkinson Disease / Q000134:cerebrospinal fluid / Q000235:genetics; D020641:Polymorphism, Single Nucleotide; D020277:Polyradiculoneuropathy, Chronic Inflammatory Demyelinating / Q000134:cerebrospinal fluid / Q000235:genetics; D024801:Tauopathies / Q000134:cerebrospinal fluid / Q000235:genetics
D015415:Biomarkers; C525030:CST3 protein, human; D055316:Cystatin C; D007840:Latex; D000409:Alanine; D005998:Glycine