Antibody signatures defined by high-content peptide microarray analysis.
Circulating antibodies are highly selective binding reagents directed to a vast repertoire of antigens. Candidate antigens displayed as overlapping peptides on microarrays can be used to screen for recognition by serum antibodies from clinically well-defined patient populations. The methodology is robust and enables unbiased visualization of antigen-specific B-cell responses. Additionally, autoantibody signatures of diagnostic value could be detected using microarrays displaying thousands of human peptides.
Masch
Antonia
A
JPT Peptide Technologies GmbH, Berlin, Germany.
Zerweck
Johannes
J
Reimer
Ulf
U
Wenschuh
Holger
H
Schutkowski
Mike
M
D000818:Animals; D000906:Antibodies / Q000097:blood / Q000276:immunology*; D005189:False Positive Reactions; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007136:Immunoglobulins / Q000276:immunology; D010455:Peptides / Q000032:analysis* / Q000276:immunology; D040081:Protein Array Analysis / Q000379:methods*
D000906:Antibodies; D007136:Immunoglobulins; D010455:Peptides