title: Crystal structure of a dimeric archaeal cleavage and polyadenylation specificity factor.
abstract: Proteins of the metallo-β-lactamase (MβL) fold form a large superfamily of metallo-hydrolase/oxidoreductases. Members of this family are found in all three domains of life and are involved in a variety of biological functions related to hydrolysis, redox processes, DNA repair and uptake, and RNA processing. We classified the archaeal homologs of this superfamily based on sequence similarity and characterized a subfamily of the Cleavage and Polyadenylation Specificity Factor (CPSF) with an uncommon domain composition: in addition to an extended MβL domain, which accommodates the active site for RNA cleavage, this group has two N-terminal KH domains. Here, we present the crystal structure of a member of this group from Methanosarcina mazei. It reveals a dimerization mode of the MβL domain that has not been observed before and suggests that RNA is bound across the dimer interface, recognized by the KH domains of one monomer, and cleaved at the active site of the other.
authors.affiliation: Department of Protein Evolution, Max-Planck-Institute for Developmental Biology, D-72076 Tübingen, Germany.
authors.forename: Bijàn
authors.initials: B
authors.lastname: Mir-Montazeri
authors.forename: Moritz
authors.initials: M
authors.lastname: Ammelburg
authors.forename: Dara
authors.initials: D
authors.lastname: Forouzan
authors.forename: Andrei N
authors.initials: AN
authors.lastname: Lupas
authors.forename: Marcus D
authors.initials: MD
authors.lastname: Hartmann
mesh_terms: D039223:Cleavage And Polyadenylation Specificity Factor / Q000235:genetics* / Q000648:ultrastructure*; D019295:Computational Biology; D003460:Crystallization; D017931:DNA Primers / Q000235:genetics; D019281:Dimerization; D017020:Methanosarcina / Q000201:enzymology*; D008958:Models, Molecular; D012313:RNA / Q000378:metabolism*; D001618:beta-Lactamases / Q000302:isolation & purification* / Q000648:ultrastructure*
chemical_list: D039223:Cleavage And Polyadenylation Specificity Factor; D017931:DNA Primers; D012313:RNA; D001618:beta-Lactamases