title: Osthole regulates inflammatory mediator expression through modulating NF-κB, mitogen-activated protein kinases, protein kinase C, and reactive oxygen species.
abstract: Osthole, a coumarin compound, has been reported to exhibit various biological activities; however the cellular mechanism of its immune modulating activity has not yet been fully addressed. In this study we isolated osthole from the seeds of Cnidium monnieri and demonstrated that osthole inhibited TNF-α, NO and COX-2 expression in LPS-stimulated macrophages, without reducing the expression of IL-6. Furthermore, the phosphorylation of p38, JNK1/2, PKC-α and PKC-ε induced by LPS was inhibited by osthole; however, the phosphorylation of ERK1/2 and PKC-δ was not reduced by osthole. Osthole also inhibited NF-κB activation and ROS release in LPS-stimulated macrophages. Our current results indicated that osthole is the major anti-inflammatory ingredient of Cnidium monnieri seed ethanol extract.
authors.affiliation: Institute of Biotechnology, National Ilan University, Ilan, Taiwan.
authors.forename: Pei-Chun
authors.initials: PC
authors.lastname: Liao
authors.forename: Shih-Chang
authors.initials: SC
authors.lastname: Chien
authors.forename: Chen-Lung
authors.initials: CL
authors.lastname: Ho
authors.forename: Eugene I-Chen
authors.initials: EI
authors.lastname: Wang
authors.forename: Shu-Ching
authors.initials: SC
authors.lastname: Lee
authors.forename: Yueh-Hsiung
authors.initials: YH
authors.lastname: Kuo
authors.forename: Narumon
authors.initials: N
authors.lastname: Jeyashoke
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
authors.forename: Wei-Chih
authors.initials: WC
authors.lastname: Dong
authors.forename: Louis Kuoping
authors.initials: LK
authors.lastname: Chao
authors.forename: Kuo-Feng
authors.initials: KF
authors.lastname: Hua
mesh_terms: D000276:Adjuvants, Immunologic; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002460:Cell Line; D031048:Cnidium / Q000737:chemistry*; D003374:Coumarins / Q000302:isolation & purification / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D051379:Mice; D020928:Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors; D016328:NF-kappa B / Q000037:antagonists & inhibitors*; D009569:Nitric Oxide / Q000096:biosynthesis; D010936:Plant Extracts / Q000737:chemistry; D011493:Protein Kinase C / Q000037:antagonists & inhibitors; D047428:Protein Kinase Inhibitors / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors*; D012639:Seeds / Q000737:chemistry; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
chemical_list: D000276:Adjuvants, Immunologic; D000893:Anti-Inflammatory Agents; D003374:Coumarins; D008070:Lipopolysaccharides; D016328:NF-kappa B; D010936:Plant Extracts; D047428:Protein Kinase Inhibitors; D017382:Reactive Oxygen Species; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; D011493:Protein Kinase C; D020928:Mitogen-Activated Protein Kinases; C046627:osthol