LC-DAD-ESI/MS(n) determination of direct condensation flavanol-anthocyanin adducts in pressure extracted pomegranate (Punica granatum L.) juice.
Pomegranate (Punica granatum L.) juice, obtained by pressure extraction of the whole fruit, has been analyzed for its flavanol-anthocyanin adduct content using reversed-phase liquid chromatography with diode array detection, coupled to mass spectrometry (ion trap) with electrospray ionization (HPLC-DAD-ESI/MS(n)), operating in positive ion mode. A total of 35 dimers have been detected, consisting of mono- and disubstituted hexoside derivatives of the adducts between the flavan-3-ols (epi)gallocatechin, (epi)catechin and (epi)afzelechin and the anthocyanidins delphinidin, cyanidin and pelargonidin. In addition, evidence is given for the presence of additional anthocyanin-flavanol adducts in this juice.
Sentandreu
Enrique
E
Instituto de Agroquímica y Tecnología de Alimentos-CSIC, Avd Agustín Escardino, 7-Parque Científico, 46980 Paterna, Valencia, Spain. jsendra@iata.csic.es
Navarro
Jose L
JL
Sendra
Jose M
JM
D000872:Anthocyanins / Q000032:analysis*; D001628:Beverages / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D005419:Flavonoids / Q000032:analysis*; D005511:Food Handling / Q000379:methods; D005638:Fruit / Q000737:chemistry*; D029561:Lythraceae / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization
D000872:Anthocyanins; D005419:Flavonoids; C404987:flavan-3-ol