The expression and function of β-1,4-galactosyltransferase-I in dendritic cells.
β-1,4-Galactosyltransferase-I (GalTI) is unusual among the galactosyltransferase family, which has two isoforms that differ only in the length of their cytoplasmic domains [1]. In this study, we found that both the long and short isoforms of GalTI were expressed in human monocyte-derived dendritic cells (MoDCs), and localized in the cytoplasm near nucleus and cytomembrane. The expression level of GalTI and cellular adhesion ability was increased when DCs continued to mature. We also demonstrated that the cellular adhesion ability of DCs was inhibited by α-lactalbumin (α-LA) via interference with cell surface GalTI function, suggesting that the adhesion ability was positively correlated with the expression of cell surface long GalTI. α-LA also could inhibit DC-T clustering and CD4(+) T cell proliferation. Collectively, the data suggests that GalTI might act as a key adhesion molecular participating in T cells-DCs contacts.
Cheng
Xiang
X
Department of Microbiology and Immunology, Medical School of Nantong University, Jiangsu, PR China.
Wang
Xiaoying
X
Han
Yu
Y
Wu
Yuanyuan
Y
D000943:Antigens, Differentiation / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology; D002448:Cell Adhesion / Q000187:drug effects / Q000276:immunology*; D002454:Cell Differentiation / Q000187:drug effects; D002462:Cell Membrane / Q000201:enzymology; D049109:Cell Proliferation / Q000187:drug effects; D003593:Cytoplasm / Q000201:enzymology; D003713:Dendritic Cells / Q000166:cytology / Q000201:enzymology* / Q000276:immunology; D005700:Galactosyltransferases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression / Q000235:genetics; D006801:Humans; D054992:Immunological Synapses / Q000201:enzymology; D007527:Isoenzymes / Q000235:genetics; D007768:Lactalbumin / Q000494:pharmacology; D007797:Laminin / Q000378:metabolism; D008213:Lymphocyte Activation / Q000187:drug effects / Q000276:immunology; D009000:Monocytes / Q000166:cytology / Q000187:drug effects
D000943:Antigens, Differentiation; D007527:Isoenzymes; D007797:Laminin; D007768:Lactalbumin; D005700:Galactosyltransferases; C119701:beta-1,4-galactosyltransferase I