Protective effect of a cysteine prodrug and antioxidant, L-2-oxothiazolidine-4-carboxylate, against ethanol-induced gastric lesions in rats.
Earlier studies have suggested an important role of glutathione (GSH) in cytoprotection against free radicals induced oxidative damage. This study reports gastroprotective effects of a cysteine precursor, L-2-oxothiazolidine-4-carboxylate (OTC), in experimental models of gastric secretion and ulceration. Acid secretion studies (volume and acidity) were undertaken in pylorus-ligated rats whereas the gastric lesions were induced by ethanol. Different groups of animals were treated with OTC (0, 100, 200 and 400 mg/kg). The levels of gastric wall mucus, nonprotein sulfhydryls (NP-SH) and myeloperoxidase (MPO) were measured in the glandular stomach of rats following ethanol-induced gastric lesions. Both medium and high doses of OTC significantly reduced the volume and acidity of gastric secretion in pylorus-ligated rats. Pretreatment with OTC significantly and dose-dependently attenuated the formation of ethanol-induced gastric lesion. OTC significantly protected the gastric mucosa against ethanol-induced depletion of gastric wall mucus, NP-SH and MPO. The gastroprotective effects of OTC may be attributed to its ability to inhibit neutrophils activity and replenish GSH demand.
Al Moutaery
Meshal
M
Prince Sultan Cardiac Center, Armed Forces Hospital, Riyadh, Saudi Arabia.
Al Rayes
Hannan
H
Al Swailam
Ramaiz
R
Elfaki
Ibrahim
I
Khan
Haseeb Ahmad
HA
Arshaduddin
Mohammad
M
Tariq
Mohammad
M
D000818:Animals; D000897:Anti-Ulcer Agents; D000975:Antioxidants / Q000494:pharmacology*; D003545:Cysteine / Q000378:metabolism; D004195:Disease Models, Animal; D000431:Ethanol / Q000633:toxicity; D005260:Female; D005753:Gastric Mucosa / Q000187:drug effects*; D008297:Male; D011355:Prodrugs / Q000494:pharmacology*; D011761:Pyrrolidonecarboxylic Acid / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013276:Stomach Ulcer / Q000139:chemically induced / Q000517:prevention & control*; D053778:Thiazolidines / Q000494:pharmacology*
D000897:Anti-Ulcer Agents; D000975:Antioxidants; D011355:Prodrugs; D053778:Thiazolidines; D000431:Ethanol; D003545:Cysteine; D011761:Pyrrolidonecarboxylic Acid; C029536:2-oxothiazolidine-4-carboxylic acid