Interferon-γ induces upregulation and activation of the interleukin-31 receptor in human dermal microvascular endothelial cells.
Interleukin-31 (IL-31), a recently discovered cytokine derived from T helper cells, is involved in chronic dermatitis and pruritus. This study demonstrates for the first time that the IL-31 receptor complex for IL-31 is substantially upregulated in human dermal microvascular endothelial cells after stimulation with interferon-γ (IFN-γ). Activation of the IL-31 receptor complex results in the induction of the intracellular ERK1/2 signaling pathway and downregulation of IFN-γ-induced monokine induced by IFN-γ expression. Inhibitor studies revealed that the IFN-γ-induced IL-31RA upregulation is processed via JNK and PI3 kinase activation. In sum, our study points toward an interaction between the T(H) 1-derived cytokine IFN-γ and the T(H) 2-derived cytokine IL-31 on endothelial cells.
Feld
Micha
M
Shpacovitch
Victoria M
VM
Fastrich
Michaela
M
Cevikbas
Ferda
F
Steinhoff
Martin
M
D002478:Cells, Cultured; D020405:Dermis / Q000098:blood supply* / Q000276:immunology* / Q000378:metabolism; D042783:Endothelial Cells / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D015870:Gene Expression / Q000276:immunology; D006801:Humans; D007371:Interferon-gamma / Q000276:immunology* / Q000494:pharmacology; D018123:Receptors, Interleukin / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D015854:Up-Regulation / Q000276:immunology
C502188:IL31RA protein, human; D018123:Receptors, Interleukin; D007371:Interferon-gamma