[Reactive oxygen species and mitochondrial membrane potential changes in leukemia cells during 6-gingerol induced apoptosis].
OBJECTIVE
To observe the effects of 6-gingerol on reactive oxygen species (ROS) and mitochondrial membrane potential(deltapsim) of chronic myeloid leukemia K562 cells and human acute T lymphoblastic leukemia MOLT4 cells, to investigate the role of mitochondrial pathway in the signal transduction of leukemia cell.


METHODS
With different concentrations of 6-gingerol treatment, using 2,7-dichloro fluoresceinciactate (DCFH-DA) as ROS probe, rhodamine-123 as deltapsim probe, the levels of ROS and deltapsim of K562 cells and MOLT4 cells were tested by flow cytomentry.


RESULTS
After treated with 6-gingerol, the ROS levels of K562 cells were significantly higher than control group (P < 0.01), while the deltapsim were significantly lower than control group (P < 0.01), and the ROS levels of MOLT4 cells were significantly higher than control group (P < 0.05).


CONCLUSIONS
6-gingerol can significantly increase ROS levels of K562 cells and MOLT4 cells, decrease deltapsim of K562 cells,induce apoptosis of leukemia cells by mitochondrial pathway.
Zeng
Hui-Lan
HL
The First Affiliated Hospital, Jinan University, Guangzhou 510632, China. thlz@jnu.edu.cn
Han
Xin-Ai
XA
Gu
Chen
C
Zhu
Hai-Yang
HY
Huang
Xue-Song
XS
Gu
Jian-Quan
JQ
Zhong
Qi
Q
Liu
Guan-Jie
GJ
Ming
Wei-Jie
WJ
Cai
Xin-Ni
XN
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D000975:Antioxidants / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D002396:Catechols / Q000008:administration & dosage / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005233:Fatty Alcohols / Q000008:administration & dosage / Q000494:pharmacology*; D005434:Flow Cytometry; D020939:Zingiber officinale / Q000737:chemistry*; D006801:Humans; D020014:K562 Cells; D007945:Leukemia, Lymphoid / Q000378:metabolism / Q000473:pathology*; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects* / Q000502:physiology; D017382:Reactive Oxygen Species / Q000378:metabolism*
D000972:Antineoplastic Agents, Phytogenic; D000975:Antioxidants; D002396:Catechols; D005233:Fatty Alcohols; D017382:Reactive Oxygen Species; C007845:gingerol