title: Genetic transformation and mutagenesis via single-stranded DNA in the unicellular, diazotrophic cyanobacteria of the genus Cyanothece.
abstract: We describe a genetic system for producing specific gene knockouts in Cyanothece sp. strain PCC 7822 using a single-stranded DNA technique (B. Zorin, P. Hegemann, and I. Sizova, Eukaryot. Cell 4:1264-1272, 2005). The first fully segregated mutant was a ΔnifK mutant, and it was unable to grow on medium lacking combined nitrogen and produced virtually no hydrogen.
authors.affiliation: Purdue University, Department of Biological Sciences, West Lafayette, IN 47907, USA.
authors.forename: Hongtao
authors.initials: H
authors.lastname: Min
authors.forename: Louis A
authors.initials: LA
authors.lastname: Sherman
mesh_terms: D003470:Culture Media / Q000737:chemistry; D046889:Cyanothece / Q000235:genetics*; D004277:DNA, Single-Stranded / Q000235:genetics*; D055786:Gene Knockout Techniques / Q000379:methods*; D005798:Genes, Bacterial; D005827:Genetics, Microbial / Q000379:methods*; D006859:Hydrogen / Q000378:metabolism; D016296:Mutagenesis; D014170:Transformation, Genetic
chemical_list: D003470:Culture Media; D004277:DNA, Single-Stranded; D006859:Hydrogen