title: Gingival crevicular fluid IL-6, tPA, PAI-2, albumin levels following initial periodontal treatment in chronic periodontitis patients with or without type 2 diabetes.
abstract: OBJECTIVE
  To evaluate initial periodontal treatment effects on gingival crevicular fluid (GCF) interleukin-6 (IL-6), tissue-type plasminogen activator (tPA), plasminogen activator inhibitor-2 (PAI-2), albumin levels in type 2 diabetic patients.
  
  
  METHODS
  GCF samples were collected from 20 type 2 diabetic, 22 non-diabetic non-smokers all with chronic periodontitis at baseline, 1-, 3-months following initial periodontal treatment. Biochemical analysis was performed by ELISA. Data were tested by Mann-Whitney U, Wilcoxon tests.
  
  
  RESULTS
  The total amounts of albumin, IL-6, tPA, PAI-2 decreased significantly in diabetics after treatment (1- and 3-months) whereas, only PAI-2 decreased in non-diabetic group at 3-months (p < 0.05). There were statistically significant differences between the diabetics and non-diabetics at all time points for albumin, PAI-2 and at 1-, 3-months for GCF volume (p < 0.050) but only at baseline for IL-6 (p < 0.050).
  
  
  CONCLUSIONS
  Present data suggest clinical improvements are less apparent in diabetic chronic periodontitis patients as reflected by disease markers in GCF and by an increase in concentrations of inflammatory proteins IL-6, tPA, and PAI-2 in GCF of this patient group following initial periodontal treatment.
authors.affiliation: Department of Periodontology, School of Dentistry, Ege University, 35100 Bornova, İzmir, Turkey.
authors.forename: Levent
authors.initials: L
authors.lastname: Kardeşler
authors.forename: Nurcan
authors.initials: N
authors.lastname: Buduneli
authors.forename: Sevki
authors.initials: S
authors.lastname: Çetinkalp
authors.forename: David
authors.initials: D
authors.lastname: Lappin
authors.forename: Denis F
authors.initials: DF
authors.lastname: Kinane
mesh_terms: D000328:Adult; D000418:Albumins / Q000378:metabolism*; D055113:Chronic Periodontitis / Q000378:metabolism / Q000628:therapy*; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005883:Gingival Crevicular Fluid / Q000737:chemistry*; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D017396:Plasminogen Activator Inhibitor 2 / Q000378:metabolism*; D010959:Tissue Plasminogen Activator / Q000378:metabolism*
chemical_list: D000418:Albumins; D015850:Interleukin-6; D017396:Plasminogen Activator Inhibitor 2; D010959:Tissue Plasminogen Activator