title: Kinetics of tissue iron in experimental autoimmune encephalomyelitis in rats.
abstract: To elucidate the role of iron in the pathomechanisms of autoimmune CNS disorders, we estimated the tissue concentrations of Fe(2+) in the brain, spinal cord, and liver in the chronic relapsing form of experimental autoimmune encephalomyelitis (EAE). The disease was induced in Dark Agouti (DA) strain of rats, by subcutaneous injection of bovine brain homogenate in complete Freund's adjuvant (CFA). Control rats consisted of unsensitized rats and of rats treated with CFA or saline. The data obtained by clinical assessment and by inductively coupled plasma spectrometry have shown that the attacks of disease (on the 12th and 22nd post-immunization day) were followed by high accumulation of iron in the liver. Additionally, during the second attack of disease, the decreased concentration of Fe(2+) was found in cervical spinal cord. The data point to regulatory effects of iron and hepatic trace elements regulating mechanisms in the pathogenesis of EAE.
authors.affiliation: Department of Chemistry and Biochemistry, Medical Faculty, University of Rijeka, B Branchetta 22, 51 000 Rijeka, Croatia.
authors.forename: Marin
authors.initials: M
authors.lastname: Tota
authors.forename: Hrvoje
authors.initials: H
authors.lastname: Jakovac
authors.forename: Damir
authors.initials: D
authors.lastname: Grebić
authors.forename: Jelena
authors.initials: J
authors.lastname: Marinić
authors.forename: Dalibor
authors.initials: D
authors.lastname: Broznić
authors.forename: Gordana
authors.initials: G
authors.lastname: Čanadi-Jurešić
authors.forename: Cedomila
authors.initials: C
authors.lastname: Milin
authors.forename: Biserka
authors.initials: B
authors.lastname: Radošević-Stašić
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000378:metabolism*; D007501:Iron / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D008297:Male; D051381:Rats; D013116:Spinal Cord / Q000378:metabolism
chemical_list: D007501:Iron