A reliable method to concentrate circulating DNA.
Concentration of circulating DNA probes is required to increase the amount of DNA involved in subsequent study (by polymerase chain reaction, sequencing, and microarray). This work was dedicated to the comparison of five different methods used for concentration of DNA circulating in blood. Precipitation of circulating DNA with acetone in the presence of triethylamine provides minimal DNA loss, high reproducibility, and at least three times higher DNA yield in comparison with the standard ethanol protocol.
Bryzgunova
Olga
O
Institute of Chemical Biology and Fundamental Medicine, Novosibirsk 630090, Russia. olga.bryzgunova@niboch.nsc.ru
Bondar
Anna
A
Morozkin
Evgeniy
E
Mileyko
Vladislav
V
Vlassov
Valentin
V
Laktionov
Pavel
P
D000096:Acetone / Q000737:chemistry; D004270:DNA, Circular / Q000097:blood / Q000737:chemistry / Q000302:isolation & purification*; D005021:Ethylamines / Q000737:chemistry; D005590:Fractional Precipitation / Q000379:methods*; D006801:Humans; D008297:Male; D011471:Prostatic Neoplasms / Q000097:blood / Q000235:genetics; D019346:Sodium Acetate / Q000737:chemistry
D004270:DNA, Circular; D005021:Ethylamines; D000096:Acetone; D019346:Sodium Acetate; C016162:triethylamine