title: Environmental factors shape sediment anammox bacterial communities in hypernutrified Jiaozhou Bay, China.
abstract: Bacterial anaerobic ammonium oxidation (anammox) is an important process in the marine nitrogen cycle. Because ongoing eutrophication of coastal bays contributes significantly to the formation of low-oxygen zones, monitoring of the anammox bacterial community offers a unique opportunity for assessment of anthropogenic perturbations in these environments. The current study used targeting of 16S rRNA and hzo genes to characterize the composition and structure of the anammox bacterial community in the sediments of the eutrophic Jiaozhou Bay, thereby unraveling their diversity, abundance, and distribution. Abundance and distribution of hzo genes revealed a greater taxonomic diversity in Jiaozhou Bay, including several novel clades of anammox bacteria. In contrast, the targeting of 16S rRNA genes verified the presence of only "Candidatus Scalindua," albeit with a high microdiversity. The genus "Ca. Scalindua" comprised the apparent majority of active sediment anammox bacteria. Multivariate statistical analyses indicated a heterogeneous distribution of the anammox bacterial assemblages in Jiaozhou Bay. Of all environmental parameters investigated, sediment organic C/organic N (OrgC/OrgN), nitrite concentration, and sediment median grain size were found to impact the composition, structure, and distribution of the sediment anammox bacterial community. Analysis of Pearson correlations between environmental factors and abundance of 16S rRNA and hzo genes as determined by fluorescent real-time PCR suggests that the local nitrite concentration is the key regulator of the abundance of anammox bacteria in Jiaozhou Bay sediments.
authors.affiliation: State Key Laboratory of Heavy Oil Processing and Centre for Bioengineering and Biotechnology, China University of Petroleum (East China), 66 West Changjiang Rd., Qingdao 266555, China. danghy@upc.edu.cn
authors.forename: Hongyue
authors.initials: H
authors.lastname: Dang
authors.forename: Ruipeng
authors.initials: R
authors.lastname: Chen
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
authors.forename: Lizhong
authors.initials: L
authors.lastname: Guo
authors.forename: Pingping
authors.initials: P
authors.lastname: Chen
authors.forename: Zuwang
authors.initials: Z
authors.lastname: Tang
authors.forename: Fang
authors.initials: F
authors.lastname: Tian
authors.forename: Shaozheng
authors.initials: S
authors.lastname: Li
authors.forename: Martin G
authors.initials: MG
authors.lastname: Klotz
mesh_terms: D001421:Bacteria, Anaerobic / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D004777:Environment; D005068:Eutrophication; D005798:Genes, Bacterial / Q000235:genetics; D019015:Geologic Sediments / Q000382:microbiology*; D008969:Molecular Sequence Data; D009792:Oceans and Seas; D010084:Oxidation-Reduction; D010802:Phylogeny; D000644:Quaternary Ammonium Compounds / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D000644:Quaternary Ammonium Compounds; D012336:RNA, Ribosomal, 16S