Predicting three-dimensional structure of protein fragments from dihedral angle propensities and molecular dynamics.
Incorporating the existing knowledge of protein structural preferences, e.g., amino acid angle frequencies, in structure prediction have proven to be less successful with smaller peptides. In this work, we compare the effectiveness of backbone angle propensity libraries derived from two protein data sets: one consisting of proteins of unrestricted lengths; the second containing proteins ranging in size from 40 to 75 residues. Model structures for 29 target peptides are predicted using a threading algorithm and their stability evaluated using in vacuo molecular dynamics simulations. Structures derived from the data set consisting of smaller proteins outperformed those developed from that unrestricted by protein length.
Blayney
Jaine K
JK
Faculty of Computing and Engineering, School of Computing and Mathematics, University of Ulster, Newtownabbey, Northern Ireland, BT37 0QB, UK. j.blayney@qub.ac.uk
Ojha
Piyush C
PC
Shapcott
Mary
M
D000465:Algorithms; D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry; D019295:Computational Biology / Q000379:methods*; D010455:Peptides / Q000737:chemistry; D055550:Protein Stability; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry*
D000596:Amino Acids; D010455:Peptides; D011506:Proteins