Functionality of sortase A in Lactococcus lactis.
Lactococcus lactis IL1403 harbors a putative sortase A (SrtA) and 11 putative sortase substrates that carry the canonical LPXTG signature of such substrates. We report here on the functionality of SrtA to anchor five LPXTG substrates to the cell wall, thus suggesting that SrtA is the housekeeping sortase in L. lactis IL1403.
Dieye
Yakhya
Y
INRA, UMR1319 Micalis, Domaine de Vilvert, F-78352 Jouy-en-Josas, France.
Oxaran
Virginie
V
Ledue-Clier
Florence
F
Alkhalaf
Walid
W
Buist
Girbe
G
Juillard
Vincent
V
Lee
Chang Won
CW
Piard
Jean-Christophe
JC
D019881:Aminoacyltransferases / Q000378:metabolism / Q000502:physiology*; D001422:Bacterial Adhesion / Q000235:genetics / Q000502:physiology; D001426:Bacterial Proteins / Q000378:metabolism / Q000502:physiology*; D002473:Cell Wall / Q000378:metabolism / Q000502:physiology; D003546:Cysteine Endopeptidases / Q000378:metabolism / Q000502:physiology*; D005798:Genes, Bacterial / Q000235:genetics; D013294:Lactococcus lactis / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D016297:Mutagenesis, Site-Directed; D021381:Protein Transport / Q000235:genetics; D020543:Proteome / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics
D001426:Bacterial Proteins; D020543:Proteome; D011993:Recombinant Fusion Proteins; D019881:Aminoacyltransferases; C120542:sortase A; D003546:Cysteine Endopeptidases