Access to phosphoproteins and glycoproteins through semi-synthesis, Native Chemical Ligation and N→S acyl transfer.
Peptide thioesters are important tools for protein synthesis and semi-synthesis through their use in Native Chemical Ligation (NCL). NCL can be employed to assemble site-specifically modified proteins that can help elucidate the mechanisms of biomolecular processes. In this article we explore the compatibility of phosphopeptide synthesis and glycopeptide synthesis with thioester production through N→S acyl transfer.
Masania
Jinit
J
Department of Chemistry, University College London, 20 Gordon Street, London, UKWC1H 0AJ.
Li
Jiejin
J
Smerdon
Stephen J
SJ
Macmillan
Derek
D
D000215:Acylation; D000595:Amino Acid Sequence; D064447:Checkpoint Kinase 2; D002851:Chromatography, High Pressure Liquid; D018360:Crystallography, X-Ray; D004952:Esters / Q000737:chemistry / Q000302:isolation & purification; D006023:Glycoproteins / Q000138:chemical synthesis* / Q000737:chemistry; D006801:Humans; D008956:Models, Chemical; D008969:Molecular Sequence Data; D009584:Nitrogen / Q000378:metabolism*; D010455:Peptides / Q000737:chemistry; D010750:Phosphoproteins / Q000138:chemical synthesis* / Q000737:chemistry; D055503:Protein Multimerization; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry; D013455:Sulfur / Q000378:metabolism*
D004952:Esters; D006023:Glycoproteins; D010455:Peptides; D010750:Phosphoproteins; D013455:Sulfur; D064447:Checkpoint Kinase 2; C578634:CHEK2 protein, human; D017346:Protein Serine-Threonine Kinases; D009584:Nitrogen