title: Diagnostic and vaccine chapter.
abstract: The first report in this chapter describes the development of a killed composite vaccine. This killed vaccine is non-infectious to humans, other animals, and the environment. The vaccine has low reactivity, is non-abortive, and does not induce pathomorphological alterations to the organs of vaccinated animals. The second report of this chapter describes the diagnostic value of a competitive enzyme-linked immunosorbent assay for detecting Brucella-specific antibodies and its ability to discriminate vaccinated cattle from infected cattle. The results indicated that the competitive enzyme-linked immunosorbent assay is more sensitive than traditional tests for detecting antibodies to Brucella abortus in naturally and experimentally infected cattle.
authors.affiliation: CRDF Technical Consultant, Arlington, VA, USA. mgoldshmidt@crdf.org
authors.forename: J H
authors.initials: JH
authors.lastname: Wolfram
authors.forename: S K
authors.initials: SK
authors.lastname: Kokanov
authors.forename: O A
authors.initials: OA
authors.lastname: Verkhovsky
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D002004:Brucella Vaccine / Q000276:immunology*; D002003:Brucella abortus / Q000145:classification; D002007:Brucellosis, Bovine / Q000175:diagnosis* / Q000276:immunology / Q000517:prevention & control*; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D058028:Research Report; D012680:Sensitivity and Specificity
chemical_list: D000907:Antibodies, Bacterial; D002004:Brucella Vaccine