Prohibitin as an oxidative stress biomarker in the eye.
Identification of biomarker proteins in the retina and retinal pigment epithelium (RPE) under oxidative stress may imply new insights into signaling mechanisms of retinal degeneration at the molecular level. Proteomic data from an in vivo mice model in constant light and an in vitro oxidative stress model are compared to controls under normal conditions. Our proteomic study shows that prohibitin is involved in oxidative stress signaling in the retina and RPE. The identity of prohibitin in the retina and RPE was studied using 2D electrophoresis, immunohistochemistry, western blot, and mass spectrometry analysis. Comparison of expression levels with apoptotic markers as well as translocation between mitochondria and the nucleus imply that the regulation of prohibitin is an early signaling event in the RPE and retina under oxidative stress. Immunohistochemical analysis of murine aged and diabetic eyes further suggests that the regulation of prohibitin in the RPE/retina is related to aging- and diabetes-induced oxidative stress. Our proteomic approach implies that prohibitin in the RPE and the retina could be a new biomarker protein of oxidative stress in aging and diabetes.
Lee
Hyunju
H
Department of Ophthalmology, University of South Carolina, Columbia, SC 29208, USA.
Arnouk
Hilal
H
Sripathi
Srinivas
S
Chen
Ping
P
Zhang
Ruonan
R
Bartoli
Manuela
M
Hunt
Richard C
RC
Hrushesky
William J M
WJ
Chung
Hyewon
H
Lee
Sung Haeng
SH
Jahng
Wan Jin
WJ
D000375:Aging / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D015415:Biomarkers / Q000378:metabolism; D002417:Cattle; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D002470:Cell Survival / Q000187:drug effects; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005123:Eye / Q000166:cytology / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008027:Light; D013058:Mass Spectrometry; D051379:Mice; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D018384:Oxidative Stress / Q000187:drug effects / Q000528:radiation effects; D000091402:Prohibitins; D021381:Protein Transport / Q000187:drug effects / Q000528:radiation effects; D040901:Proteomics; D051381:Rats; D017207:Rats, Sprague-Dawley; D012097:Repressor Proteins / Q000378:metabolism*; D055213:Retinal Pigment Epithelium / Q000166:cytology / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D013347:Subcellular Fractions / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects
D015415:Biomarkers; D000091402:Prohibitins; D012097:Repressor Proteins; D006861:Hydrogen Peroxide