title: MIQE précis: Practical implementation of minimum standard guidelines for fluorescence-based quantitative real-time PCR experiments.
abstract: The conclusions of thousands of peer-reviewed publications rely on data obtained using fluorescence-based quantitative real-time PCR technology. However, the inadequate reporting of experimental detail, combined with the frequent use of flawed protocols is leading to the publication of papers that may not be technically appropriate. We take the view that this problem requires the delineation of a more transparent and comprehensive reporting policy from scientific journals. This editorial aims to provide practical guidance for the incorporation of absolute minimum standards encompassing the key assay parameters for accurate design, documentation and reporting of qPCR experiments (MIQE précis) and guidance on the publication of pure 'reference gene' articles.
authors.forename: Stephen A
authors.initials: SA
authors.lastname: Bustin
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Beaulieu
authors.forename: Jim
authors.initials: J
authors.lastname: Huggett
authors.forename: Rolf
authors.initials: R
authors.lastname: Jaggi
authors.forename: Frederick S B
authors.initials: FS
authors.lastname: Kibenge
authors.forename: Pål A
authors.initials: PA
authors.lastname: Olsvik
authors.forename: Louis C
authors.initials: LC
authors.lastname: Penning
authors.forename: Stefan
authors.initials: S
authors.lastname: Toegel
mesh_terms: D005453:Fluorescence; D017712:Peer Review, Research / Q000592:standards; D010506:Periodicals as Topic / Q000592:standards; D016133:Polymerase Chain Reaction / Q000295:instrumentation / Q000379:methods / Q000592:standards; D011643:Publishing / Q000592:standards