Comparison of the Sensititre YeastOne® dilution method with the Clinical Laboratory Standards Institute (CLSI) M27-A3 microbroth dilution reference method for determining MIC of eight antifungal agents on 102 yeast strains.
The Clinical Laboratory Standards Institute ([CLSI] formerly NCCLS) reference broth microdilution testing method (protocol M27-A3) was compared with a commercially available methods (Sensititre YeastOne(®)) by testing two quality control strains and 102 isolates of Candida sp. and Cryptococcus sp. against fluconazole, itraconazole, ketoconazole, posaconazole, voriconazole, flucytosin, amphotericin B and caspofungin. Minimal inhibitory concentrations (MIC) endpoints were determined after 24h of incubation for Sensititre YeastOne(®) and after 24 and 48 h for CLSI microdilution method. Essential agreements between methods vary from 70.6 to 92.2%. Categorical agreements vary from 94.1% for 5FC to 72.6% for AMB. Sensititre YeastOne(®) reading appears to be useful for avoiding very major errors and this confirms the interest of this method for evaluating new antifungals activity in vitro.
Bertout
S
S
UMR 145 (IRD/UM1), UFR sciences pharmaceutiques et biologiques, 15, avenue Charles-Flahault, BP 14491, 34093 Montpellier cedex 5, France.
Dunyach
C
C
Drakulovski
P
P
Reynes
J
J
Mallié
M
M
D000935:Antifungal Agents / Q000494:pharmacology*; D002175:Candida / Q000187:drug effects / Q000254:growth & development; D003454:Cryptococcus / Q000187:drug effects / Q000254:growth & development; D003470:Culture Media; D008826:Microbial Sensitivity Tests / Q000379:methods*; D015203:Reproducibility of Results; D015003:Yeasts / Q000187:drug effects* / Q000254:growth & development
D000935:Antifungal Agents; D003470:Culture Media