Catalytic biomineralization of fluorescent calcite by the thermophilic bacterium Geobacillus thermoglucosidasius.
The thermophilic Geobacillus bacterium catalyzed the formation of 100-μm hexagonal crystals at 60°C in a hydrogel containing sodium acetate, calcium chloride, and magnesium sulfate. Under fluorescence microscopy, crystals fluoresced upon excitation at 365 ± 5, 480 ± 20, or 545 ± 15 nm. X-ray diffraction indicated that the crystals were magnesium-calcite in calcite-type calcium carbonate.
Yoshida
Naoto
N
Department of Biochemistry and Applied Biosciences, University of Miyazaki, 1-1 Gakuen Kibanadai-Nishi, Miyazaki 889-2192, Japan. a04109u@cc.miyazaki-u.ac.jp
Higashimura
Eiji
E
Saeki
Yuichi
Y
D002113:Calcification, Physiologic; D002119:Calcium Carbonate / Q000378:metabolism*; D002122:Calcium Chloride / Q000378:metabolism; D002384:Catalysis; D003460:Crystallization; D056506:Geobacillus / Q000378:metabolism*; D008278:Magnesium Sulfate / Q000378:metabolism; D008856:Microscopy, Fluorescence; D019346:Sodium Acetate / Q000378:metabolism; D014961:X-Ray Diffraction
D019346:Sodium Acetate; D008278:Magnesium Sulfate; D002119:Calcium Carbonate; D002122:Calcium Chloride