Role of Rho-ROCK signaling in MOLT4 cells metastasis induced by CCL25.
Our previous research has revealed that binding of the chemokine CCL25 to the CCR9 provides chemotactic cues guiding leukemic cells to specific tissues and organs. The RhoA-ROCK pathway might be involved in cancer migration. The purpose of this study was to explore the role of the RhoA-ROCK-MLC axis in leukemic cell migration following exposure to CCL25. The results showed that CCL25 could increase the amount of the GTPase RhoA and activate MLC in MOLT4 cells in a time-dependent manner. C3 exoenzyme and Y-27632 could block MOLT4 cell migration and chemotaxis. Thus, the RhoA-ROCK-MLC axis might play an important role in MOLT4 cell metastasis induced by CCL25.
Zhang
Li
L
Department of Immunology, College of Basic Medical Science, Wuhan University, 185 Donghu Road, Wuhan, 430071 Hubei, China.
Yu
Beibei
B
Hu
Meng
M
Wang
Zhan
Z
Liu
Dongying
D
Tong
Xiaoling
X
Leng
Jun
J
Zhou
Beibei
B
Hu
Yi
Y
Wu
Ranran
R
Ding
Qianshan
Q
Zhang
Qiuping
Q
D002460:Cell Line; D019742:Chemokines, CC / Q000502:physiology*; D006801:Humans; D009362:Neoplasm Metastasis; D054460:rho-Associated Kinases / Q000378:metabolism*; D020742:rhoA GTP-Binding Protein / Q000378:metabolism*
C108074:CCL25 protein, human; D019742:Chemokines, CC; C515851:ROCK1 protein, human; D054460:rho-Associated Kinases; D020742:rhoA GTP-Binding Protein