title: Chiral separation of the β2-sympathomimetic fenoterol by HPLC and capillary zone electrophoresis for pharmacokinetic studies.
abstract: The development of methods for the separation of the enantiomers of fenoterol by chiral HPLC and capillary zone electrophoresis (CZE) is described. For the HPLC separation precolumn fluorescence derivatization with naphthyl isocyanate was applied. The resulting urea derivatives were resolved on a cellulose tris(3,5-dimethylphenylcarbamate)-coated silica gel column employing a column switching procedure. Detection was carried out fluorimetrically with a detection limit in the low ng/mL range. The method was adapted to the determination of fenoterol enantiomers in rat heart perfusates using liquid-liquid extraction. As an alternative a CE method was used for the direct separation of fenoterol enantiomers comparing different cyclodextrin derivatives as chiral selectors.
authors.affiliation: Faculty of Biosciences, Institute of Biochemistry and Biotechnology, Martin Luther University Halle-Wittenberg, Kurt-Mothes-Str. 3, D-06120 Halle (Saale), Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Ullrich
authors.forename: Dirk
authors.initials: D
authors.lastname: Wesenberg
authors.forename: Corinna
authors.initials: C
authors.lastname: Bleuel
authors.forename: Gerd-Joachim
authors.initials: GJ
authors.lastname: Krauss
authors.forename: Martin G
authors.initials: MG
authors.lastname: Schmid
authors.forename: Michael
authors.initials: M
authors.lastname: Weiss
authors.forename: Gerald
authors.initials: G
authors.lastname: Gübitz
mesh_terms: D058666:Adrenergic beta-2 Receptor Agonists / Q000737:chemistry / Q000302:isolation & purification; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000379:methods*; D005280:Fenoterol / Q000737:chemistry* / Q000302:isolation & purification; D009206:Myocardium / Q000737:chemistry; D051381:Rats; D013237:Stereoisomerism; D013566:Sympathomimetics / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D058666:Adrenergic beta-2 Receptor Agonists; D013566:Sympathomimetics; D005280:Fenoterol