Identification of piRNAs in Hela cells by massive parallel sequencing.
Piwi proteins and Piwi-interacting RNAs (piRNAs) have been implicated in transposon control in germline from Drosophila to mammals. To examine the profile of small RNA expression in human cancer cells and explore difference in small RNA transcriptome, small RNA libraries prepared from wildtype, HILI overexpressed and HILI knockdowned Hela cells were sequenced using Solexa technology. piRNAs and other repeat- associated small RNAs were observed in Hela cells. By using in situ hybridization, piR-49322 was localized in the nucleolus and around the periphery of nuclear membrane in Hela cells. Following the overexpression of HILI, the retrotransposon elements LINE1 was significantly repressed, while LINE1-associated small RNAs decreased in abundance. The present study demonstrated that HILI along with piRNAs plays a role in LINE1 suppression in Hela cancer cell line.
Lu
Yilu
Y
Department of Medical Genetics, West China Hospital, Sichuan University, Chengdu, China.
Li
Chao
C
Zhang
Kun
K
Sun
Huaqin
H
Tao
Dachang
D
Liu
Yunqiang
Y
Zhang
Sizong
S
Ma
Yongxin
Y
D006367:HeLa Cells; D006801:Humans; D017403:In Situ Hybridization; D020084:Long Interspersed Nucleotide Elements / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017423:Sequence Analysis, RNA
D034741:RNA, Small Interfering