Tube gel isotachophoresis: a method for quantitative isolation of nucleic acids from diluted solutions.
The technique of isotachophoresis is intended for separation of molecules having different electrophoretic mobilities in a nonhomogeneous electric field. Since the mobility of nucleic acids in water solutions is uniform and does not depend on their size (because of a uniform distribution of negatively charged phosphate groups along the molecule), isotachophoresis will concentrate rather than separate them in the mobile borderline zone between the rapid (Cl(-)) and the slow (β-alanine(-)) anions. This idea served as the basis for elaboration of a novel method for isolation of nucleic acids from diluted solutions. Advantages of the method include quantitative yield (regardless of molecule size), high degree of concentration, and the ability to visually monitor the process. The method may find applications in nucleic acid isolation from highly degraded forensic and clinical samples, from bodily fluids in particular, and thereby promote development of this important direction of diagnostics.
Kondratova
Valentina N
VN
Blokhin Russian Cancer Research Center, Russian Academy of Medical Sciences, Kashirskoe Shosse 24, 115478 Moscow, Russia.
Botezatu
Irina V
IV
Shelepov
Valery P
VP
Lichtenstein
Anatoly V
AV
D000838:Anions / Q000737:chemistry; D004247:DNA / Q000097:blood* / Q000302:isolation & purification; D005470:Fluorometry / Q000379:methods; D053803:Forensic Genetics; D005782:Gels / Q000737:chemistry*; D006801:Humans; D057625:Isotachophoresis / Q000379:methods*; D010710:Phosphates / Q000737:chemistry; D012996:Solutions / Q000737:chemistry
D000838:Anions; D005782:Gels; D010710:Phosphates; D012996:Solutions; D004247:DNA