Gene silencing mediated by magnetic lipospheres tagged with small interfering RNA.
Lipospheres made from soy bean oil and a combination of the cationic lipid Metafectene and the helper lipid dioleoylphosphatidyl-ethanolamine were functionalized with magnetic nanoparticles (NPs) and small interfering RNA (siRNA). The resulting magnetic lipospheres loaded with siRNA are proven here as efficient nonviral vectors for gene silencing. Embedding magnetic NPs in the shell of lipospheres allows for magnetic force-assisted transfection (magnetofection) as well as magnetic targeting in both static and fluidic conditions mimicking the bloodstream.
del Pino
Pablo
P
Fachbereich Physik und Wissenschaftliches Zentrum für Materialwissenschaften, Philipps Universität Marburg, Renthof 7, 35037 Marburg, Germany.
Munoz-Javier
Almudena
A
Vlaskou
Dialechti
D
Rivera Gil
Pilar
P
Plank
Christian
C
Parak
Wolfgang J
WJ
D000818:Animals; D020868:Gene Silencing; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D008055:Lipids / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry*; D008280:Magnetics; D051379:Mice; D041681:NIH 3T3 Cells; D053758:Nanoparticles / Q000737:chemistry*; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010938:Plant Oils / Q000737:chemistry; D034741:RNA, Small Interfering / Q000008:administration & dosage*; D013025:Glycine max / Q000737:chemistry
D008055:Lipids; D008081:Liposomes; D010714:Phosphatidylethanolamines; D010938:Plant Oils; D034741:RNA, Small Interfering; C500466:metafectene; D049452:Green Fluorescent Proteins; C094877:dioleoyl phosphatidylethanolamine