title: [Effect of electroacupuncture at "Ciliao"(BL 32) on c-fos expression in the sacral segment of spinal cord in rats with detrusor hyperreflexia].
abstract: OBJECTIVE
  To observe the effect of electroacupuncture (EA) at "Ciliao" (BL 32) on detrusor hyperreflexia and c-fos expression in the sacral segment of spinal cord in rats with spinal cord injury (SCI).
  
  
  METHODS
  Thirty-seven adult female Sprague-Dawley rats were randomized into normal control (n=5), SCI model (n=16) and EA (n=16) groups. EA (20 Hz, 3 mA) was applied to bilateral BL 32 for 2 hours, once daily for 14 days. Intravesical pressure was detected by using a pressure transducer and a bioelectric amplifier. The expression of c-fos gene was detected by immunohistochemistry.
  
  
  RESULTS
  In comparison with normal control group, the maximum intravesical pressure (MIVP) raised significantly in model group (P < 0.05), and the compliance of the bladder decreased remarkably (P < 0.05). While compared with model group, MIVP decreased significantly in SCI rats of EA group after EA intervention (P < 0.05), and the vesical compliance increased obviously (P < 0.05). In comparison with normal control group, the mean optical density (OD) value of c-fos immuno-reaction (IR) positive products increased significantly in the sacral cord after SCI in model group (P < 0.05), while compared with model group, the mean OD value of c-fos IR positive products in EA group declined evidently but still being higher than that of normal control group (P < 0.05), displaying a downregulation of c-fos expression after EA.
  
  
  CONCLUSIONS
  Electroacupuncture at "Ciliao" (BL 32) can inhibit the overactivity of bladder in SCI rats and reduce the c-fos expression in the sacral cord, suggesting that the declined C-fibers' activity after EA may be one of its mechanism underlying improving detrusor hyperreflexia in spinal cord injury.
authors.affiliation: Department of Acu-moxibustion, Guanganmen Hospital of China Academy of Chinese Medical Sciences, Beijing 100053, China. ayujinnaa@sina.com
authors.forename: Jin-na
authors.initials: JN
authors.lastname: Yu
authors.forename: Xiao-jing
authors.initials: XJ
authors.lastname: Ma
authors.forename: Zhi-shun
authors.initials: ZS
authors.lastname: Liu
authors.forename: Zhen-yu
authors.initials: ZY
authors.lastname: Wu
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhang
authors.forename: Guang-jun
authors.initials: GJ
authors.lastname: Wu
authors.forename: Yan-hua
authors.initials: YH
authors.lastname: Deng
authors.forename: Bao-guang
authors.initials: BG
authors.lastname: Gu
mesh_terms: D015669:Acupuncture Points; D000818:Animals; D015671:Electroacupuncture; D015870:Gene Expression; D006801:Humans; D008297:Male; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012021:Reflex, Abnormal; D013116:Spinal Cord / Q000378:metabolism*; D053201:Urinary Bladder, Overactive / Q000235:genetics / Q000378:metabolism / Q000628:therapy*
chemical_list: D016760:Proto-Oncogene Proteins c-fos