title: Cytotoxic 3,4-seco-Atisane Diterpenoids from Croton barorum and Croton goudotii.
abstract: In a screening program directed to the discovery of new anticancer agents from Madagascan plants, ethyl acetate extracts of Croton barorum and C. goudotii showed strong cytotoxic activity, with 100% inhibition at 10 μg/mL in a primary screen using the murine lymphocytic leukemia P388 cell line. Bioassay-guided fractionation led to the isolation of two new 3,4-seco-atisane diterpenoids, crotobarin (1) and crotogoudin (2). Their structures were elucidated by spectroscopic data interpretation. Compounds 1 and 2 produced a net progression in the number of cells arrested at the G2/M growth stage in the cell cycle of the K562 human leukemia cell line at 4 μM.
authors.affiliation: Laboratoire de Biothérapeutique, Institut Malgache des Recherches Appliquées, BP 3833, 101 Antananarivo, Madagascar.
authors.forename: Olga L
authors.initials: OL
authors.lastname: Rakotonandrasana
authors.forename: Fanja H
authors.initials: FH
authors.lastname: Raharinjato
authors.forename: Mialy
authors.initials: M
authors.lastname: Rajaonarivelo
authors.forename: Vincent
authors.initials: V
authors.lastname: Dumontet
authors.forename: Marie-Thérèse
authors.initials: MT
authors.lastname: Martin
authors.forename: Jérôme
authors.initials: J
authors.lastname: Bignon
authors.forename: Philippe
authors.initials: P
authors.lastname: Rasoanaivo
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D029802:Croton / Q000737:chemistry* / Q000235:genetics; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D020014:K562 Cells; D008270:Madagascar; D051379:Mice; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D004224:Diterpenes; C000600506:crotobarin; C000600507:crotogoudin