Clerodendron glandulosum.Coleb extract ameliorates high fat diet/fatty acid induced lipotoxicity in experimental models of non-alcoholic steatohepatitis.
This study evaluates the protective role of Clerodendron glandulosum.Coleb (CG) aqueous extract against high fat diet/fatty acid induced lipotoxicity in experimental models of non-alcoholic steatohepatitis (NASH). Supplementation of NASH mice with CG extract (1% and 3% in high fat diet for 16 weeks) prevented high fat diet induced elevation in liver enzymes, plasma and hepatic lipids, mitochondrial oxidative stress and compromised enzymatic and non-enzymatic antioxidant status and histopathological damage to hepatocytes. Furthermore, results from in vitro study indicates, addition of CG extract (20-200 μg/ml for 24h) to HepG2 cells minimizes oleic acid induced lipid accumulation, higher lipid peroxidation, cytotoxicity and reduced cell viability. These in vivo and in vitro studies suggest that CG extract has the potential of preventing high fat/fatty acid induced NASH.
Jadeja
Ravirajsinh N
RN
Division of Phytotherapeutics and Metabolic Endocrinology, Department of Zoology, Faculty of Science, The MS University of Baroda, Vadodara, Gujarat, India.
Thounaojam
Menaka C
MC
Dandekar
Deven S
DS
Devkar
Ranjitsinh V
RV
Ramachandran
A V
AV
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D000975:Antioxidants / Q000032:analysis / Q000494:pharmacology; D001219:Aspartate Aminotransferases / Q000097:blood; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D032409:Clerodendrum / Q000737:chemistry*; D004032:Diet; D004041:Dietary Fats / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D005227:Fatty Acids / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D005234:Fatty Liver / Q000139:chemically induced* / Q000188:drug therapy* / Q000473:pathology; D050356:Lipid Metabolism / Q000187:drug effects; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism
D000975:Antioxidants; D004041:Dietary Fats; D005227:Fatty Acids; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase