Molecular cloning of cyanobacterial pteridine glycosyltransferases that catalyze the transfer of either glucose or xylose to tetrahydrobiopterin.
Here, we report cloning of cyanobacterial genes encoding pteridine glycosyltransferases that catalyze glucosyl or xylosyl transfer from UDP-sugars to tetrahydrobiopterin. The genes were cloned by PCR amplification from genomic DNA which was isolated from culture and environmental samples and overexpressed in Escherichia coli for an in vitro activity assay.
Lee
Yeol Gyun
YG
School of Biological Sciences, Inje University, Kimhae 621-749, South Korea.
Kim
Ae Hyun
AH
Park
Mi Bi
MB
Kim
Hye-Lim
HL
Lee
Kon Ho
KH
Park
Young Shik
YS
D000595:Amino Acid Sequence; D001708:Biopterins / Q000031:analogs & derivatives* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D003001:Cloning, Molecular; D016000:Cluster Analysis; D000458:Cyanobacteria / Q000201:enzymology* / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D004783:Environmental Microbiology; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D005947:Glucose / Q000378:metabolism*; D016695:Glycosyltransferases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014994:Xylose / Q000378:metabolism*
D004269:DNA, Bacterial; D001708:Biopterins; D014994:Xylose; D016695:Glycosyltransferases; C003402:sapropterin; D005947:Glucose