title: Increase of MT1-MMP, TIMP-2 and Ki-67 proteins in the odontogenic region of the rat incisor post-shortening procedure.
abstract: MT1-MMP and TIMP-2 are well known for their roles in remodelling of extracellular matrix components. However, reports are emerging on the involvement of these molecules in cell kinetics. In the rat incisor tooth, a shortening treatment increases the eruption and cell proliferation rates. However, the role of MT1-MMP and TIMP-2 proteins in these processes is still to be evaluated. Male Wistar rats were divided in two groups. In the normofunctional group (NF) the lower teeth of the rats remained in a normal eruption process. In the hypofunctional group (HP) rats their lower left incisor tooth was shortened every 2 days during 12 days. The eruption rate was estimated during the shortening period and MT1-MMP, TIMP-2 and Ki-67 protein expression from the odontogenic region was measured after the treatment. In HP groups an increase in eruption rate, and in MT1-MMP/TIMP-2 and Ki-67 expression were observed. We conclude that there is a relationship between the increase in eruption rate, and in levels of MT1-MMP, TIMP-2 and Ki-67 in the HP group. This suggests that MT1-MMP and TIMP-2 may have some role in cell proliferation during the eruption of the rat incisor tooth.
authors.affiliation: Departamento de Biologia Estrutural Molecular e Genética, UEPG, Carlos Cavalcanti Avenue, 4748 Ponta Grossa, PR 84030-900, Brazil. osteoblasto@ig.com.br
authors.forename: Jose Rosa
authors.initials: JR
authors.lastname: Gomes
authors.forename: Nádia Fayez
authors.initials: NF
authors.lastname: Omar
authors.forename: Juliana
authors.initials: J
authors.lastname: Dos Santos Neves
authors.forename: Eliene Aparecida Orsini
authors.initials: EA
authors.lastname: Narvaes
authors.forename: Pedro Duarte
authors.initials: PD
authors.lastname: Novaes
mesh_terms: D000818:Animals; D015153:Blotting, Western; D049109:Cell Proliferation; D005786:Gene Expression Regulation; D007150:Immunohistochemistry; D007180:Incisor; D019394:Ki-67 Antigen / Q000378:metabolism*; D008297:Male; D053511:Matrix Metalloproteinase 14 / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000378:metabolism*
chemical_list: D019394:Ki-67 Antigen; D019716:Tissue Inhibitor of Metalloproteinase-2; D053511:Matrix Metalloproteinase 14