title: Mechanism of uranium (VI) removal by two anaerobic bacterial communities.
abstract: The mechanism of uranium (VI) removal by two anaerobic bacterial consortia, recovered from an uncontaminated site (consortium A) and other from an uranium mine (consortium U), was investigated. The highest efficiency of U (VI) removal by both consortia (97%) occurred at room temperature and at pH 7.2. Furthermore, it was found that U (VI) removal by consortium A occurred by enzymatic reduction and bioaccumulation, while the enzymatic process was the only mechanism involved in metal removal by consortium U. FTIR analysis suggested that after U (VI) reduction, U (IV) could be bound to carboxyl, phosphate and amide groups of bacterial cells. Phylogenetic analysis of 16S rRNA showed that community A was mainly composed by bacteria closely related to Sporotalea genus and Rhodocyclaceae family, while community U was mainly composed by bacteria related to Clostridium genus and Rhodocyclaceae family.
authors.affiliation: Centro de Ciências do Mar, Universidade do Algarve, FCT-DQF (edifício 8), Campus de Gambelas, 8005-139 Faro, Portugal.
authors.forename: Mónica
authors.initials: M
authors.lastname: Martins
authors.affiliation: IBB - Centro de Biomedicina Molecular e Estrutural, Universidade do Algarve, FCT, Campus de Gambelas, 8005-139 Faro, Portugal.
authors.forename: Maria Leonor
authors.initials: ML
authors.lastname: Faleiro
authors.affiliation: Centro de Investigação em Química do Algarve, Universidade do Algarve, FCT, DQF, Campus de Gambelas, 8005-139 Faro, Portugal.
authors.forename: Ana M Rosa
authors.initials: AMR
authors.lastname: da Costa
authors.affiliation: Universidade de Lisboa, Faculdade de Ciências, Centro de Biodiversidade, Genómica Integrativa e Funcional (BioFIG), Campus de FCUL, Campo Grande, 1749-016 Lisboa, Portugal.
authors.forename: Sandra
authors.initials: S
authors.lastname: Chaves
authors.affiliation: Universidade de Lisboa, Faculdade de Ciências, Centro de Biodiversidade, Genómica Integrativa e Funcional (BioFIG), Campus de FCUL, Campo Grande, 1749-016 Lisboa, Portugal.
authors.forename: Rogério
authors.initials: R
authors.lastname: Tenreiro
authors.affiliation: Serviço de Anatomia Patológica, Hospital Curry Cabral, Lisboa, Portugal.
authors.forename: António Pedro
authors.initials: AP
authors.lastname: Matos
authors.affiliation: Centro de Ciências do Mar, Universidade do Algarve, FCT-DQF (edifício 8), Campus de Gambelas, 8005-139 Faro, Portugal. Electronic address: mcorada@ualg.pt.
authors.forename: Maria Clara
authors.initials: MC
authors.lastname: Costa
mesh_terms: D001421:Bacteria, Anaerobic / Q000145:classification / Q000378:metabolism*; D001483:Base Sequence; D017931:DNA Primers; D006863:Hydrogen-Ion Concentration; D046529:Microscopy, Electron, Transmission; D010084:Oxidation-Reduction; D010802:Phylogeny; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D014501:Uranium / Q000302:isolation & purification* / Q000378:metabolism; D014961:X-Ray Diffraction
chemical_list: D017931:DNA Primers; D014501:Uranium