title: An inter-laboratory validation of methods of lipid peroxidation measurement in UVA-treated human plasma samples.
abstract: Lipid peroxidation products like malondialdehyde, 4-hydroxynonenal and F(2)-isoprostanes are widely used as markers of oxidative stress in vitro and in vivo. This study reports the results of a multi-laboratory validation study by COST Action B35 to assess inter-laboratory and intra-laboratory variation in the measurement of lipid peroxidation. Human plasma samples were exposed to UVA irradiation at different doses (0, 15 J, 20 J), encoded and shipped to 15 laboratories, where analyses of malondialdehyde, 4-hydroxynonenal and isoprostanes were conducted. The results demonstrate a low within-day-variation and a good correlation of results observed on two different days. However, high coefficients of variation were observed between the laboratories. Malondialdehyde determined by HPLC was found to be the most sensitive and reproducible lipid peroxidation product in plasma upon UVA treatment. It is concluded that measurement of malondialdehyde by HPLC has good analytical validity for inter-laboratory studies on lipid peroxidation in human EDTA-plasma samples, although it is acknowledged that this may not translate to biological validity.
authors.affiliation: Institute of Biological Chemistry and Nutrition, University of Hohenheim, Stuttgart, Germany.
authors.forename: Nicolle
authors.initials: N
authors.lastname: Breusing
authors.forename: Tilman
authors.initials: T
authors.lastname: Grune
authors.forename: Luka
authors.initials: L
authors.lastname: Andrisic
authors.forename: Mustafa
authors.initials: M
authors.lastname: Atalay
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Bartosz
authors.forename: Fiorella
authors.initials: F
authors.lastname: Biasi
authors.forename: Suzana
authors.initials: S
authors.lastname: Borovic
authors.forename: Laura
authors.initials: L
authors.lastname: Bravo
authors.forename: Isidre
authors.initials: I
authors.lastname: Casals
authors.forename: Rosario
authors.initials: R
authors.lastname: Casillas
authors.forename: Anca
authors.initials: A
authors.lastname: Dinischiotu
authors.forename: Joanna
authors.initials: J
authors.lastname: Drzewinska
authors.forename: Heidemarie
authors.initials: H
authors.lastname: Faber
authors.forename: Norsyahida Mohd
authors.initials: NM
authors.lastname: Fauzi
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Gajewska
authors.forename: Juan
authors.initials: J
authors.lastname: Gambini
authors.forename: Daniela
authors.initials: D
authors.lastname: Gradinaru
authors.forename: Tarja
authors.initials: T
authors.lastname: Kokkola
authors.forename: Antonin
authors.initials: A
authors.lastname: Lojek
authors.forename: Wojciech
authors.initials: W
authors.lastname: Luczaj
authors.forename: Denisa
authors.initials: D
authors.lastname: Margina
authors.forename: Cinzia
authors.initials: C
authors.lastname: Mascia
authors.forename: Raquel
authors.initials: R
authors.lastname: Mateos
authors.forename: Andreas
authors.initials: A
authors.lastname: Meinitzer
authors.forename: María Teresa
authors.initials: MT
authors.lastname: Mitjavila
authors.forename: Lidija
authors.initials: L
authors.lastname: Mrakovcic
authors.forename: Maria Cristina
authors.initials: MC
authors.lastname: Munteanu
authors.forename: Martina
authors.initials: M
authors.lastname: Podborska
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Poli
authors.forename: Paulina
authors.initials: P
authors.lastname: Sicinska
authors.forename: Elzbieta
authors.initials: E
authors.lastname: Skrzydlewska
authors.forename: Jose
authors.initials: J
authors.lastname: Vina
authors.forename: Ingrid
authors.initials: I
authors.lastname: Wiswedel
authors.forename: Neven
authors.initials: N
authors.lastname: Zarkovic
authors.forename: Sieglinde
authors.initials: S
authors.lastname: Zelzer
authors.forename: Corinne M
authors.initials: CM
authors.lastname: Spickett
mesh_terms: D000447:Aldehydes / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods / Q000592:standards; D002853:Chromatography, Liquid / Q000379:methods / Q000592:standards; D019963:Clinical Chemistry Tests / Q000379:methods / Q000592:standards*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000592:standards; D006801:Humans; D028421:Isoprostanes / Q000032:analysis*; D015227:Lipid Peroxidation / Q000502:physiology*; D008315:Malondialdehyde / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods / Q000592:standards; D010949:Plasma / Q000737:chemistry / Q000528:radiation effects*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014466:Ultraviolet Rays
chemical_list: D000447:Aldehydes; D028421:Isoprostanes; D008315:Malondialdehyde; C027576:4-hydroxy-2-nonenal