[Activation of TLR3 pathway in the pathogenesis of nephrotic syndrome induced by respiratory syncytial virus in rat model].
OBJECTIVE
To explore the activation of toll-like receptor 3 (TLR3) pathway on the process of respiratory syncytial virus (RSV) induced nephropathy.


METHODS
SD rats were inoculated intranasally and intraperitoneally with 6 X 10(6) plaque forming unit(PFU) RSV and sacrificed on days 4, 14, 30 postinoculation (RSV4, RSV14 and RSV30). The normal rats without intervention were set as control. Renal tissues were obtained, and the morphological changes were studied. The expressions of TLR3, NF-kappaB and IL-13 in the rats' kidney were measured with real-time quantitative RT-PCR, and indirect IF staining.


RESULTS
After the inoculation of RSV, the rats had proteinuria and the fusion of foot processes of glomerular epithelial cells, resembling human minimal changed nephrotic syndrome (MCNS). The expressions of TLR3, NF-kappaB and IL-13 in renal tissues increased obviously at Day 4 and Day 14 postinoculation, the differences were significant when compared with normal control rats (P < 0.05). The expressions of these three factors decreased at Day 30, which were not significantly different to those of normal control group (P > 0.05).


CONCLUSIONS
TLR3 signal pathway may play an important role in early stage of RSV nephropathy.
Wu
Jin
J
Department of Pediatrics, West China Second Hospital, Sichuan University, Chengdu 610041, China.
Wang
Zheng
Z
Guo
Yan-Nan
YN
Dong
Li-Qun
LQ
D000818:Animals; D008297:Male; D009404:Nephrotic Syndrome / Q000209:etiology / Q000378:metabolism* / Q000821:virology*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D018357:Respiratory Syncytial Virus Infections / Q000378:metabolism / Q000821:virology*; D012136:Respiratory Syncytial Viruses / Q000502:physiology*; D015398:Signal Transduction; D051196:Toll-Like Receptor 3 / Q000235:genetics / Q000378:metabolism*
C530472:TLR3 protein, rat; D051196:Toll-Like Receptor 3