Detection of Helicobacter rodentium-like DNA in the liver tissue of patients with chronic liver diseases by polymerase chain reaction-denaturing gradient gel electrophoresis and DNA sequence analysis.
Many Helicobacter spp. were isolated from the stomach, intestinal tract, and liver of different animals and humans. The association between Helicobacter spp. and hepatobiliary diseases, including hepatocellular carcinoma, was thoroughly examined, indicating a potential role of the bacteria in the progression toward cancer. In our work, we screened 97 liver biopsies from patients with chronic liver diseases for the presence of Helicobacter spp. DNA. With the use of genus-specific polymerase chain reaction (PCR)-denaturing gradient gel electrophoresis (DGGE) and subsequent sequencing, we found that the majority of Helicobacter spp. DNA detected was similar to Helicobacter rodentium DNA (71%). The DNA of other detected Helicobacter spp. was similar to Helicobacter pylori DNA. This is the first indication of H. rodentium-like DNA presence in human liver tissue. We also conclude that PCR-DGGE is a useful screening method for assigning species designation and heterogeneity.
Nakonieczna
Joanna
J
Laboratory of Molecular Diagnostics, Department of Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Gdansk, Poland. strzala@biotech.ug.gda.pl
Stalke
Piotr
P
Al-Soud
Waleed Abu
WA
Wadström
Torkel
T
Bielawski
Krzysztof Piotr
KP
D000293:Adolescent; D000328:Adult; D000368:Aged; D001431:Bacteriological Techniques; D001706:Biopsy; D002908:Chronic Disease; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification*; D058645:Denaturing Gradient Gel Electrophoresis; D005260:Female; D016998:Helicobacter / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D016481:Helicobacter Infections / Q000175:diagnosis* / Q000382:microbiology; D006801:Humans; D008107:Liver Diseases / Q000382:microbiology*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D055815:Young Adult
D004269:DNA, Bacterial