title: Preparation and characterization of iron-containing liposomes: their effect on soluble iron uptake by Caco-2 cells.
abstract: The aim of this work was to study the iron uptake of Caco-2 cells incubated with five different formulations of liposomes containing iron. The vesicles were also characterized before, during, and after in vitro digestion. Caco-2 cells were incubated with digested and nondigested liposomes, and soluble iron uptake was determined. Nondigested liposomes made with chitosan (CHI) or the cationic lipid, DC-Cholesterol (DC-CHOL), generated the highest iron uptake. However, these two formulations were highly unstable under in vitro digestion, resulting in nonmeasurable iron uptake. Digested conventional liposomes composed of soybean phosphatidylcholine (SPC), hydrogentated phosphatidylcholine (HSPC), or HSPC and cholesterol (CHOL) presented the highest iron-uptake values. These liposomal formulations protected iron from oxidation and improved iron uptake from intestinal cells, compared to an aqueous solution of ferrous sulphate.
authors.affiliation: Center of Research and Development in Chemistry, National Institute of Industrial Technology, Buenos Aires, Argentina.
authors.forename: Laura G
authors.initials: LG
authors.lastname: Hermida
authors.forename: Anna
authors.initials: A
authors.lastname: Roig
authors.forename: Carlos
authors.initials: C
authors.lastname: Bregni
authors.forename: Manuel
authors.initials: M
authors.lastname: Sabés-Xamaní
authors.forename: Ramon
authors.initials: R
authors.lastname: Barnadas-Rodríguez
mesh_terms: D018938:Caco-2 Cells / Q000378:metabolism*; D048271:Chitosan / Q000737:chemistry; D002784:Cholesterol / Q000737:chemistry; D006801:Humans; D007501:Iron / Q000737:chemistry* / Q000378:metabolism*; D019266:Iron, Dietary / Q000378:metabolism; D008081:Liposomes / Q000737:chemistry*; D010316:Particle Size
chemical_list: D019266:Iron, Dietary; D008081:Liposomes; D048271:Chitosan; D002784:Cholesterol; D007501:Iron