A proteome map of axoglial specializations isolated and purified from human central nervous system.
Compact myelin, the paranode, and the juxtaparanode are discrete domains that are formed on myelinated axons. In humans, neurological disorders associated with loss of myelin, including Multiple Sclerosis, often also result in disassembly of the node of Ranvier. Despite the importance of these domains in the proper functioning of the CNS, their molecular composition and assembly mechanism remains largely unknown. We therefore performed a large-scale proteomics MudPIT screen for the identification of proteins in human myelin and axogliasomal fractions. We identified over 1,000 proteins in these fractions. Since even minor perturbations in neuron-glial interactions can uncouple the glial support of axons, the proteome map presented here can be used as a reference library for "myelin health" and disease states, including white matter disorders such as leukodystrophies and multiple sclerosis.
Dhaunchak
Ajit S
AS
The Montreal Neurological Institute and Hospital, McGill University, Department of Neurology and Neurosurgery, Montreal H3A2B4, Canada. david.colman@mcgill.ca
Huang
Jeffrey K
JK
De Faria Junior
Omar
O
Roth
Alejandro D
AD
Pedraza
Liliana
L
Antel
Jack P
JP
Bar-Or
Amit
A
Colman
David R
DR
D000328:Adult; D002458:Cell Fractionation; D002490:Central Nervous System / Q000378:metabolism* / Q000473:pathology / Q000648:ultrastructure; D006801:Humans; D056784:Leukoencephalopathies / Q000378:metabolism / Q000473:pathology; D008854:Microscopy, Electron; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000378:metabolism* / Q000473:pathology; D009419:Nerve Tissue Proteins / Q000302:isolation & purification / Q000378:metabolism*; D009836:Oligodendroglia / Q000378:metabolism* / Q000473:pathology / Q000648:ultrastructure; D040901:Proteomics; D011901:Ranvier's Nodes / Q000378:metabolism* / Q000473:pathology / Q000648:ultrastructure; D055815:Young Adult
D009419:Nerve Tissue Proteins