Regulation of replication termination by Reb1 protein-mediated action at a distance.
DNA transactions driven by long-range protein-mediated inter- and intrachromosomal interactions have been reported to influence gene expression. Here, we report that site-specific replication termination in Schizosaccharomyces pombe is modulated by protein-mediated interactions between pairs of Ter sites located either on the same or on different chromosomes. The dimeric Reb1 protein catalyzes termination and mediates interaction between Ter sites. The Reb1-dependent interactions between two antiparallel Ter sites in cis caused looping out of the intervening DNA in vitro and enhancement of fork arrest in vivo. A Ter site on chromosome 2 interacted pairwise with two Ter sites located on chromosome 1 by chromosome kissing. Mutational inactivation of the major interacting Ter site on chromosome 1 significantly reduced fork arrest at the Ter site on chromosome 2, thereby revealing a cooperative mechanism of control of replication termination.
Singh
Samarendra K
SK
Department of Molecular Biology and Biochemistry, Medical University of South Carolina, Charleston, SC 29425, USA.
Sabatinos
Sarah
S
Forsburg
Susan
S
Bastia
Deepak
D
D015825:Chromosomes, Fungal; D004261:DNA Replication; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005090:Exodeoxyribonucleases / Q000378:metabolism; D012045:Regulatory Sequences, Nucleic Acid; D012568:Schizosaccharomyces / Q000378:metabolism*; D029702:Schizosaccharomyces pombe Proteins / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism*
D004268:DNA-Binding Proteins; D029702:Schizosaccharomyces pombe Proteins; D014157:Transcription Factors; C512907:reb1 protein, S pombe; D005090:Exodeoxyribonucleases; C020133:exodeoxyribonuclease III