Oct4 regulates the miR-302 cluster in P19 mouse embryonic carcinoma cells.
Oct4 is a transcription factor that is required for pluripotency during early embryogenesis and the maintenance of embryonic stem (ES) cell and pluripotent cell identity. miR-302, a cluster of eight microRNAs (miRNAs) that are expressed specifically in ES cells and pluripotent cells, is crucial for normal pluripotent cell self-renewal and pluripotency. But, the mechanism by which miR-302 participates in the core regulatory circuitry that controls self-renewal and pluripotency in P19 embryonic carcinoma cells has not been established. Here, we show that Oct4 is required for the expression and transcriptional activation of miR-302 and that Oct4 binds to the putative promoter of miR-302, suggesting that Oct4 activates the primary miR-302 transcript in P19 cells. This study proposes that the miR-302 cluster acts downstream of the Oct4 regulation network in P19 cells.
Liu
Haiying
H
Department of Reproductive Medicine, First Affiliated Hospital of Harbin Medical University, Harbin, 150001 Heilongjiang, People's Republic of China.
Deng
Suo
S
Zhao
Zihan
Z
Zhang
Huiya
H
Xiao
Juxia
J
Song
Wei
W
Gao
Fei
F
Guan
Yongmei
Y
D000818:Animals; D045744:Cell Line, Tumor; D047369:Chromatin Immunoprecipitation; D054278:Embryonal Carcinoma Stem Cells / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D051379:Mice; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D005810:Multigene Family; D050814:Octamer Transcription Factor-3 / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding; D014158:Transcription, Genetic
C558265:MIRN302 microRNA, mouse; D035683:MicroRNAs; D050814:Octamer Transcription Factor-3; C494174:Pou5f1 protein, mouse