Self-assembled fluorescent magnetic nanoprobes for multimode-biomedical imaging.
We fabricated multimode nanoprobes for acquisition of biological information at different object levels, i.e., in vivo detection and ex vivo validation for characterizing tumor angiogenesis. Fluorescent magnetic nanoprobes (FMNPs) were synthesized by using amphiphilic pyrenyl polyethylene glycol (Py-PEG) and superparamagnetic MnFe(2)O(4) nanocrystals (MNCs). Py-PEG, which is synthesized by conjugation of hydrophilic PEG with hydrophobic and fluorescent 1-pyrenebutyric acid through an esterification process, is capable of self-assembly and maintaining a high UV fluorescent intensity in aqueous phase. Py-PEG can be used as a fluorescent surfactant that simultaneously and efficiently encapsulates MNCs to exhibit fluorescent and magnetic properties as well as maintaining high water-solubility. Consequently, we proved that our biologically non-toxic FMNPs were prominent multimode imaging probes by showing not only excellent MR sensitivity but also high illumination intensity with strong signal strength under short exposure time of UV light from the extensive imaging studies of in vitro/vivo and ex vivo using orthotopic and xenograft mice models.
Lim
Eun-Kyung
EK
Department of Chemical and Biomolecular Engineering, College of Engineering, Yonsei University, Seoul, Republic of Korea.
Yang
Jaemoon
J
Dinney
Colin P N
CP
Suh
Jin-Suck
JS
Huh
Yong-Min
YM
Haam
Seungjoo
S
D000818:Animals; D002460:Cell Line; D045744:Cell Line, Tumor; D005453:Fluorescence; D008279:Magnetic Resonance Imaging / Q000295:instrumentation*; D008280:Magnetics; D008297:Male; D017895:Manganese Compounds / Q000737:chemistry; D051379:Mice; D008856:Microscopy, Fluorescence; D008962:Models, Theoretical; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000295:instrumentation* / Q000379:methods; D011092:Polyethylene Glycols / Q000737:chemistry
D017895:Manganese Compounds; D011092:Polyethylene Glycols