Robust light emission from cyclic alkylaminoluciferin substrates for firefly luciferase.
Firefly luciferase utilizes the chemical energy of ATP and oxygen to convert its substrate, D-luciferin, into an excited-state oxyluciferin molecule. Relaxation of this molecule to the ground state is responsible for the yellow-green light emission. Synthetic cyclic alkylaminoluciferins that allow robust red-shifted light emission with the modified luciferase Ultra-Glo are described. Overall light emission is higher than that of acyclic alkylaminoluciferins, aminoluciferin, and the native substrate D-luciferin.
Reddy
Gadarla Randheer
GR
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation Street, Worcester, Massachusetts 01605, USA.
Thompson
Walter C
WC
Miller
Stephen C
SC
D000255:Adenosine Triphosphate / Q000737:chemistry; D000818:Animals; D052160:Benzothiazoles / Q000737:chemistry; D048888:Fireflies / Q000201:enzymology*; D049454:Firefly Luciferin / Q000138:chemical synthesis / Q000737:chemistry*; D008027:Light; D049409:Luciferases, Firefly / Q000737:chemistry / Q000378:metabolism*; D049408:Luminescent Agents / Q000138:chemical synthesis / Q000737:chemistry*; D015394:Molecular Structure; D010100:Oxygen / Q000737:chemistry; D013237:Stereoisomerism; D013379:Substrate Specificity
D052160:Benzothiazoles; C532924:D-luciferin; D049408:Luminescent Agents; D049454:Firefly Luciferin; D000255:Adenosine Triphosphate; D049409:Luciferases, Firefly; D010100:Oxygen