title: Simultaneous determination of disulfiram and bupropion in human plasma of alcohol and nicotine abusers.
abstract: An isocratic high-performance liquid-chromatographic method has been developed for the simultaneous determination of disulfiram and bupropion in human plasma samples. Analyses were carried out on a C(8) reversed-phase column using a mobile phase composed of 50% acetonitrile and 50% aqueous phosphate buffer, containing triethylamine. Diode-array detection was used, operating at a wavelength of 250 nm. For the clean-up of plasma samples, a solid phase extraction procedure, based on C(2) cartridges, was implemented. Extraction yields of the analytes were satisfactory, being always higher than 84%. The calibration curve was linear over the 5-500 ng mL(-1) plasma concentration range for both disulfiram and bupropion. The method showed a high sensitivity (limit of detection of 1.5 ng mL(-1)) and satisfactory precision, selectivity and accuracy. The application to human plasma samples obtained from some alcohol and nicotine abusers also gave good results.
authors.affiliation: Department of Pharmaceutical Sciences, University of Bologna, Via Belmeloro 6, 40126 Bologna, Italy.
authors.forename: Maria Addolorata
authors.initials: MA
authors.lastname: Saracino
authors.forename: Chiara
authors.initials: C
authors.lastname: Marcheselli
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Somaini
authors.forename: Gilberto
authors.initials: G
authors.lastname: Gerra
authors.forename: Francesco
authors.initials: F
authors.lastname: De Stefano
authors.forename: Maria Chiara
authors.initials: MC
authors.lastname: Pieri
authors.forename: Maria Augusta
authors.initials: MA
authors.lastname: Raggi
mesh_terms: D000437:Alcoholism / Q000097:blood*; D016642:Bupropion / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004221:Disulfiram / Q000097:blood*; D006801:Humans; D015394:Molecular Structure; D014029:Tobacco Use Disorder / Q000097:blood*
chemical_list: D016642:Bupropion; D004221:Disulfiram