Response analysis of stimulating efficacy of polihexanide in an in vitro wound model with respiratory ciliary epithelial cells.
In animal wound models, accelerated wound closure has been shown by use of polihexanide applied in antimicrobially effective concentrations. Additionally, an increased ATP production of keratinocytes in vitro induced by polihexanide was demonstrated and interpreted as a stimulatory effect on cell proliferation. Based on these results and the clinical reports on improved wound healing after introduction of polihexanide for preoperative antisepsis in the nasal cavity, polihexanide was tested in a wound model on respiratory ciliary epithelial cells allowing measurement of the healing process after artificial injury. 0.5 μg/ml polihexanide accelerated wound healing in terms of proliferation and migration significantly after an exposure time of 1 and 96 h. At a concentration of 1 μg/ml polihexanide, the stimulation of wound healing was significantly increased only after an exposure time of 96 h. This is the first study to demonstrate acceleration of wound healing in a standardized in vitro model using an epithelial cell line. Considering the present results and previous reports on the impact of polihexanide on wound healing, the conclusion is drawn that the positive effect of polihexanide on wound healing is a separate, dose-dependent effect independent of its antiseptic properties.
Roth
C
C
Department of Otorhinolaryngology, Head and Neck Surgery, Ernst Moritz Arndt University Greifswald, Greifswald, Germany.
Beule
A G
AG
Kramer
A
A
Hosemann
W
W
Kohlmann
T
T
Scharf
C
C
D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D001645:Biguanides / Q000494:pharmacology*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells / Q000502:physiology*; D006801:Humans; D012137:Respiratory System; D014945:Wound Healing / Q000187:drug effects*; D014946:Wound Infection / Q000188:drug therapy*
D000891:Anti-Infective Agents, Local; D001645:Biguanides; C031233:polihexanide