Design, construction and characterization of a set of insulated bacterial promoters.
We have generated a series of variable-strength, constitutive, bacterial promoters that act predictably in different sequence contexts, span two orders of magnitude in strength and contain convenient sites for cloning and the introduction of downstream open-reading frames. Importantly, their design insulates these promoters from the stimulatory or repressive effects of many 5'- or 3'-sequence elements. We show that different promoters from our library produce constant relative levels of two different proteins in multiple genetic contexts. This set of promoters should be a useful resource for the synthetic-biology community.
Davis
Joseph H
JH
Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Rubin
Adam J
AJ
Sauer
Robert T
RT
D001419:Bacteria / Q000235:genetics*; D015723:Gene Library; D017930:Genes, Reporter; D005818:Genetic Engineering; D056426:Genetic Loci; D038101:Insulator Elements; D016366:Open Reading Frames; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic