Enantiomeric separation by capillary electrochromatography on a sulfated poly β-cyclodextrin modified silica-based monolith.
A sulfated poly β-cyclodextrin (SPCD) modified silica-based monolithic column was prepared for enantiomeric separation. First, 2-hydroxy-3-allyloxy-propyl-β-cyclodextrin (allyl-β-CD) was bonded onto a bifunctional reagent 3-(methacryloxy)propyltriethoxysilane (γ-MAPS) modified silica-based monolith through radical polymerization; the column was then sulfated with chlorosulfonic acid. The SPCD chiral stationary phase resolved the boring problem associated with desalting when sulfated CDs were synthesized to act as chiral additives. The inorganic salt in the column introduced during the sulfating process could be easily removed by washing the column with water for some time. Chiral compounds investigated were successfully resolved into their enantiomers on the SPCD modified monolith in the capillary electrochromatography (CEC) mode. Due to the existence of the -SO(3)H group, electrosmotic flow (EOF) was obviously increased, and all of the separations could be carried out in 20 min with only a minor loss in the column efficiency and resolution.
Yuan
Ruijuan
R
School of Chinese Pharmacy, Beijing University of Chinese Medicine, PR China. rjyuance@yahoo.com.cn
Wang
Yan
Y
Ding
Guosheng
G
D053801:Capillary Electrochromatography; D003505:Cyclodextrins / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D008689:Methacrylates; D058105:Polymerization; D012821:Silanes; D012822:Silicon Dioxide / Q000737:chemistry / Q000378:metabolism; D013237:Stereoisomerism; D013431:Sulfates / Q000737:chemistry / Q000378:metabolism; D047392:beta-Cyclodextrins / Q000737:chemistry / Q000378:metabolism
D003505:Cyclodextrins; D007202:Indicators and Reagents; D008689:Methacrylates; D012821:Silanes; D013431:Sulfates; D047392:beta-Cyclodextrins; C017492:methacryloxypropyltrimethoxysilane; D012822:Silicon Dioxide; C031215:betadex