Short communication: evidence of HIV type 1 clade C env clones containing low V3 loop charge obtained from an AIDS patient in India that uses CXCR6 and CCR8 for entry in addition to CCR5.
Abstract HIV-1 clade C is the major subtype circulating in India and preferentially uses CCR5 during the entire disease course. We have recently shown that env clones from an Indian patient; NARI-VB105 uses multiple coreceptors for entry and was presented with an unusual V3 loop sequence giving rise to high net V3 loop positive charges. Here we show that env clones belonging to subtype C obtained from an AIDS patient, NARI-VB52, use CXCR6 and CCR8 in addition to CCR5 for entry. However, unlike the NARI-105 patient, the env clones contained a low V3 loop net charge of +3 with a conserved GPGQ motif typical of CCR5 using subtype C strains, indicating that residues outside the V3 loop contributed to extended coreceptor use in this particular patient.
Gharu
Lavina
L
Department of Molecular Virology, National AIDS Research Institute, Bhosari, Pune, India.
Ringe
Rajesh
R
Satyakumar
Anupindi
A
Patil
Ajit
A
Bhattacharya
Jayanta
J
D000163:Acquired Immunodeficiency Syndrome / Q000821:virology*; D015686:Gene Products, env / Q000235:genetics*; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D007194:India; D008969:Molecular Sequence Data; D019713:Receptors, CCR5 / Q000502:physiology*; D054401:Receptors, CCR8 / Q000502:physiology*; D000075744:Receptors, CXCR6; D019707:Receptors, Chemokine / Q000502:physiology*; D011991:Receptors, Virus / Q000502:physiology*
C103330:CCR8 protein, human; C106330:CXCR6 protein, human; D015686:Gene Products, env; D019713:Receptors, CCR5; D054401:Receptors, CCR8; D000075744:Receptors, CXCR6; D019707:Receptors, Chemokine; D011991:Receptors, Virus