title: Partial least squares analysis and mixture design for the study of the influence of composition variables on lipidic nanoparticle characteristics.
abstract: Lipidic nanoparticles (NP), formulated from a phase inversion temperature process, have been studied with chemometric techniques to emphasize the influence of the four major components (Solutol®, Labrasol®, Labrafac®, water) on their average diameter and their distribution in size. Typically, these NP present a monodisperse size lower than 200 nm, as determined by dynamic light scattering measurements. From the application of the partial least squares (PLS) regression technique to the experimental data collected during definition of the feasibility zone, it was established that NP present a core-shell structure where Labrasol® is well encapsulated and contributes to the structuring of the NP. Even if this solubility enhancer is regarded as a pure surfactant in the literature, it appears that the oil moieties of this macrogolglyceride mixture significantly influence its properties. Furthermore, results have shown that PLS technique can be also used for predictions of sizes for given relative proportions of components and it was established that from a mixture design, the quantitative mixture composition to use in order to reach a targeted size and a targeted polydispersity index (PDI) can be easily predicted. Hence, statistical models can be a useful tool to control and optimize the characteristics in size of NP.
authors.affiliation: Centre d'Etudes et de Recherche sur le Médicament de Normandie, UPRES EA 4258, FR CNRS 3038 INC3M, UFR des Sciences Pharmaceutiques, Université de Caen Basse-Normandie, bd Becquerel, 14032 Caen Cedex, France. aurelie.malzert-freon@unicaen.fr
authors.forename: A
authors.initials: A
authors.lastname: Malzert-Fréon
authors.forename: D
authors.initials: D
authors.lastname: Hennequin
authors.forename: S
authors.initials: S
authors.lastname: Rault
mesh_terms: D005989:Glycerides; D016018:Least-Squares Analysis; D008055:Lipids / Q000737:chemistry*; D015233:Models, Statistical; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D009930:Organic Chemicals / Q000737:chemistry; D010316:Particle Size; D012995:Solubility
chemical_list: D005989:Glycerides; D008055:Lipids; D009930:Organic Chemicals; C440220:Labrasol