title: Xyn10A, a thermostable endoxylanase from Acidothermus cellulolyticus 11B.
abstract: We cloned and purified the major family 10 xylanase (Xyn10A) from Acidothermus cellulolyticus 11B. Xyn10A was active on oat spelt and birchwood xylans between 60°C and 100°C and between pH 4 and pH 8. The optimal activity was at 90°C and pH 6; specific activity and K(m) for oat spelt xylan were 350 μmol xylose produced min⁻¹ mg of protein⁻¹ and 0.53 mg ml⁻¹, respectively. Based on xylan cleavage patterns, Xyn10A is an endoxylanase, and its half-life at 90°C was approximately 1.5 h in the presence of xylan.
authors.affiliation: Department of Plant Sciences, University of California, Davis, CA 95616, USA.
authors.forename: Ravi D
authors.initials: RD
authors.lastname: Barabote
authors.forename: Juanito V
authors.initials: JV
authors.lastname: Parales
authors.forename: Ying-Yi
authors.initials: YY
authors.lastname: Guo
authors.forename: John M
authors.initials: JM
authors.lastname: Labavitch
authors.forename: Rebecca E
authors.initials: RE
authors.lastname: Parales
authors.forename: Alison M
authors.initials: AM
authors.lastname: Berry
mesh_terms: D000192:Actinomycetales / Q000201:enzymology* / Q000235:genetics; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D043364:Endo-1,4-beta Xylanases / Q000378:metabolism*; D005798:Genes, Bacterial / Q000235:genetics; D006207:Half-Life; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D014990:Xylans / Q000378:metabolism
chemical_list: D004269:DNA, Bacterial; D014990:Xylans; D043364:Endo-1,4-beta Xylanases