lacZ reporter system for use in Borrelia burgdorferi.
Regulation of gene expression is critical for the ability of Borrelia burgdorferi to adapt to different environments during its natural infectious cycle. Reporter genes have been used successfully to study gene regulation in multiple organisms. We have introduced a lacZ gene into B. burgdorferi, and we show that B. burgdorferi produces a protein with detectable β-galactosidase activity in both liquid and solid media when lacZ is expressed from a constitutive promoter. Furthermore, when lacZ is expressed from the ospC promoter, β-galactosidase activity is detected only in B. burgdorferi clones that express ospC, and it accurately monitors endogenous gene expression. The addition of lacZ to the repertoire of genetic tools available for use in B. burgdorferi should contribute to a better understanding of how B. burgdorferi gene expression is regulated during the infectious cycle.
Hayes
Beth M
BM
Laboratory of Zoonotic Pathogens, Rocky Mountain Laboratories, Hamilton, MT 59840, USA. hayesb@niaid.nih.gov
Jewett
Mollie W
MW
Rosa
Patricia A
PA
D025065:Borrelia burgdorferi / Q000235:genetics* / Q000502:physiology; D015964:Gene Expression Regulation, Bacterial; D017930:Genes, Reporter; D005827:Genetics, Microbial / Q000379:methods*; D011401:Promoter Regions, Genetic; D001616:beta-Galactosidase / Q000235:genetics* / Q000378:metabolism*
D001616:beta-Galactosidase