title: Dynamic in vivo mapping of model moisturiser ingress into human skin by GARfield MRI.
abstract: We describe the development of in vivo one-dimensional MRI (profiling) using a GARField (Gradient At Right angles to Field) magnet for the characterisation of side-of-hand human skin. For the first time and in vivo, we report measurements of the NMR longitudinal and transverse relaxation parameters and self-diffusivity of the upper layers of human skin with a nominal spatial resolution better than 10 µm. The results are correlated with in vivo confocal Raman spectroscopy measurements of water concentration and natural moisturiser factors, and discussed in terms of known skin biology and microstructure of the stratum corneum and viable epidermis. The application of model moisturiser solutions to the skin is followed and their dynamics of ingress are characterised using the MRI methodology developed. Selected hydrophilic and lipophilic formulations are studied. The results are corroborated by standard in vivo measurements of transepidermal water loss and hydration status. A further insight into moisturisation mechanisms is gained. The effect of two different penetration enhancers on a commonly used skin care oil is also discussed, and different timescales of oil penetration into the skin are reported depending on the type of enhancer.
authors.affiliation: Unilever Discover, Colworth Science Park, Sharnbrook, Bedford, UK. elisabetta.ciampi@unilever.com
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Ciampi
authors.forename: Michael
authors.initials: M
authors.lastname: van Ginkel
authors.forename: Peter J
authors.initials: PJ
authors.lastname: McDonald
authors.forename: Simon
authors.initials: S
authors.lastname: Pitts
authors.forename: Eleanor Y M
authors.initials: EY
authors.lastname: Bonnist
authors.forename: Scott
authors.initials: S
authors.lastname: Singleton
authors.forename: Ann-Marie
authors.initials: AM
authors.lastname: Williamson
mesh_terms: D000328:Adult; D004058:Diffusion; D006262:Health; D006801:Humans; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D012867:Skin / Q000187:drug effects*; D012879:Skin Physiological Phenomena / Q000187:drug effects*; D013997:Time Factors; D014867:Water / Q000494:pharmacology*
chemical_list: D014867:Water