Applications of loop-mediated isothermal DNA amplification.
During the last 10 years, with the development of loop-mediated isothermal amplification (LAMP) method, it has been widely applied in nucleic acid analysis because of its simplicity, rapidity, high efficiency, and outstanding specificity. This method employs a DNA polymerase and a set of four specially designed primers that recognize a total of six distinct sequences on the target DNA. Expensive equipment are not necessary to acquire a high level of precision, and there are fewer preparation steps compared to conventional PCR and real-time PCR assays. This paper briefly summarized the applications of LAMP method in pathogenic microorganisms, genetically modified ingredients, tumor detection, and embryo sex identification.
Fu
Shijun
S
Key Laboratory of Preventive Veterinary Medicine and Animal Biotechnology, Binzhou Animal Husbandry and Veterinary Research Academy, Binzhou 256600, People's Republic of China. fsj729@yahoo.com.cn
Qu
Guanggang
G
Guo
Shijin
S
Ma
Lin
L
Zhang
Na
N
Zhang
Songlin
S
Gao
Sanyang
S
Shen
Zhiqiang
Z
D000818:Animals; D004247:DNA / Q000032:analysis* / Q000235:genetics; D017931:DNA Primers / Q000737:chemistry / Q000235:genetics*; D004259:DNA-Directed DNA Polymerase / Q000235:genetics / Q000378:metabolism*; D042241:Early Diagnosis; D005260:Female; D005333:Fetus; D030841:Food, Genetically Modified; D006801:Humans; D058889:Molecular Typing / Q000379:methods; D009369:Neoplasms / Q000175:diagnosis; D021141:Nucleic Acid Amplification Techniques / Q000379:methods; D012680:Sensitivity and Specificity; D012732:Sex Determination Analysis
D017931:DNA Primers; D004247:DNA; D004259:DNA-Directed DNA Polymerase