α,α'-Dihydroxyketone formation using aromatic and heteroaromatic aldehydes with evolved transketolase enzymes.
Transketolase mutants have been identified that accept aromatic acceptors with good stereoselectivities, in particular benzaldehyde for which the wild type enzyme showed no activity.
Galman
James L
JL
Department of Chemistry, University College London, 20 Gordon Street, London WC1H 0AJ, UK.
Steadman
David
D
Bacon
Sarah
S
Morris
Phattaraporn
P
Smith
Mark E B
ME
Ward
John M
JM
Dalby
Paul A
PA
Hailes
Helen C
HC
D000447:Aldehydes / Q000378:metabolism; D001547:Benzaldehydes / Q000378:metabolism; D002384:Catalysis; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D007659:Ketones / Q000378:metabolism*; D009154:Mutation; D013237:Stereoisomerism; D013379:Substrate Specificity; D014174:Transketolase / Q000235:genetics / Q000378:metabolism*
D000447:Aldehydes; D001547:Benzaldehydes; D007659:Ketones; D014174:Transketolase; C032175:benzaldehyde