Characterization of a bacterial strain capable of degrading DDT congeners and its use in bioremediation of contaminated soil.
A bacterial strain DDT-6 (D6) capable of utilizing dichlorodiphenyltrichloroethane (DDT), dichlorodiphenyldichloroethane (DDD), and dichlorodiphenyldichloroethylene (DDE) (DDTs) as its sole carbon and energy source was isolated and identified as Sphingobacterium sp. The degradation of DDTs by strain D6 in mineral salt medium and in field soil was investigated. The half-lives of the degradation of DDTs increased with increasing concentration ranging from 1 to 50 mg L(-1). Favorable degradation conditions for DDTs by strain D6 were found to be pH 7.0 and 30°C. The degradation of DDTs by strain D6 was found to be statistically significantly enhanced (p ≤ 0.05) by the addition of glucose. Based on the metabolites detected, a pathway was proposed for DDT degradation in which it undergoes dechlorination, hydrogenation, dioxygenation, decarboxylation, hydroxylation, and phenyl ring-cleavage reactions to complete the mineralization process. The addition of strain D6 into the contaminated soils was found to statistically significantly enhance (p ≤ 0.05) the degradation of DDTs. The results indicate that the isolate D6 can be used successfully for the removal or detoxification of residues of DDTs in contaminated soil.
Fang
Hua
H
Department of Plant Protection, College of Agriculture & Biotechnology, Zhejiang University, Hangzhou 310029, Zhejiang, PR China.
Dong
Bin
B
Department of Plant Protection, College of Agriculture & Biotechnology, Zhejiang University, Hangzhou 310029, Zhejiang, PR China.
Yan
Hu
H
Department of Plant Protection, College of Agriculture & Biotechnology, Zhejiang University, Hangzhou 310029, Zhejiang, PR China.
Tang
Feifan
F
Department of Plant Protection, College of Agriculture & Biotechnology, Zhejiang University, Hangzhou 310029, Zhejiang, PR China.
Yu
Yunlong
Y
Department of Plant Protection, College of Agriculture & Biotechnology, Zhejiang University, Hangzhou 310029, Zhejiang, PR China. Electronic address: ylyu@zju.edu.cn.
D001673:Biodegradation, Environmental; D003634:DDT / Q000378:metabolism*; D052918:Environmental Restoration and Remediation / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D007306:Insecticides / Q000378:metabolism*; D013058:Mass Spectrometry; D012989:Soil Pollutants / Q000378:metabolism*; D013696:Temperature
D007306:Insecticides; D012989:Soil Pollutants; D003634:DDT