Metal binding to a zinc-finger peptide: a comparison between solution and the gas phase.
Solution-phase spectroscopy and mass spectrometry are used to probe interactions between divalent metal ions and a synthetic Cys(2)His(2) zinc-finger peptide (vCP1). Both methods provide the same order of binding affinity, zinc ≥ cobalt ≫ copper ≫ calcium. Collision-cross-section measurements show that both apo and holo forms are compact. This is corroborated by molecular-dynamics simulations.
Berezovskaya
Yana
Y
School of Chemistry, University of Edinburgh, Edinburgh, UK.
Armstrong
Craig T
CT
Boyle
Aimee L
AL
Porrini
Massimiliano
M
Woolfson
Derek N
DN
Barran
Perdita E
PE
D002118:Calcium / Q000737:chemistry*; D003035:Cobalt / Q000737:chemistry*; D003300:Copper / Q000737:chemistry*; D003545:Cysteine / Q000737:chemistry; D005740:Gases / Q000737:chemistry; D006639:Histidine / Q000737:chemistry; D008958:Models, Molecular; D056004:Molecular Dynamics Simulation; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry*; D012996:Solutions; D015032:Zinc / Q000737:chemistry*; D016335:Zinc Fingers
D005740:Gases; D010455:Peptides; D012996:Solutions; D003035:Cobalt; D006639:Histidine; D003300:Copper; D015032:Zinc; D003545:Cysteine; D002118:Calcium