Salt concentration effects on equilibrium melting curves from DNA microarrays.
DNA microarrays find applications in an increasing number of domains where more quantitative results are required. DNA being a charged polymer, the repulsive interactions between the surface of the microarray and the targets in solution are increasing upon hybridization. Such electrostatic penalty is generally reduced by increasing the salt concentration. In this article, we present equilibrium-melting curves obtained from dedicated physicochemical experiments on DNA microarrays in order to get a better understanding of the electrostatic penalty incurred during the hybridization reaction at the surface. Various salt concentrations have been considered and deviations from the commonly used Langmuir adsorption model are experimentally quantified for the first time in agreement with theoretical predictions.
Fuchs
J
J
Structure et Propriétés d'Architectures Moleculaires UMR 5819, Commissariat à l'Energie Atomique et aux Energies Alternatives, Centre National de la Recherche Scientifique, Université Joseph Fourier, and Institute for Nanoscience and Cryogenics, Grenoble, France.
Fiche
J-B
JB
Buhot
A
A
Calemczuk
R
R
Livache
T
T
D001483:Base Sequence; D055598:Chemical Phenomena / Q000187:drug effects; D004247:DNA / Q000737:chemistry* / Q000187:drug effects / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D006046:Gold / Q000737:chemistry; D009691:Nucleic Acid Denaturation / Q000187:drug effects; D009693:Nucleic Acid Hybridization / Q000187:drug effects; D020411:Oligonucleotide Array Sequence Analysis; D015345:Oligonucleotide Probes / Q000737:chemistry / Q000235:genetics; D058105:Polymerization / Q000187:drug effects; D011758:Pyrroles / Q000737:chemistry; D012492:Salts / Q000494:pharmacology*; D055672:Static Electricity; D013455:Sulfur / Q000737:chemistry; D013816:Thermodynamics
D015345:Oligonucleotide Probes; D011758:Pyrroles; D012492:Salts; D013455:Sulfur; D006046:Gold; D004247:DNA