RIP3 and AtKinesin-13A - a novel interaction linking Rho proteins of plants to microtubules.
RIP3 belongs to a group of recently identified proteins, classified as the ICR/RIP family whose members were described to interact with Rho proteins of plants (ROPs). Our in vivo and in vitro data demonstrate that RIP3 is a true ROP effector, interacting specifically with the active form of ROPs. We found that RIP3 has properties and cellular roles different from the previously described RIP family member ICR1/RIP1. We show that RIP3 is localized at microtubules and interacts with the kinesin-13 family member AtKinesin-13A, suggesting a role for RIP3 in microtubule reorganization and a possible function in ROP-regulated polar growth.
Mucha
Elena
E
Max Planck Institute of Molecular Physiology, Structural Biology Department, Otto Hahn Strasse 11, 44227 Dortmund, Germany.
Hoefle
Caroline
C
Hückelhoven
Ralph
R
Berken
Antje
A
D017360:Arabidopsis / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D002151:Calorimetry; D002850:Chromatography, Gel; D031541:Fluorescence Resonance Energy Transfer; D008870:Microtubules / Q000378:metabolism*; D011485:Protein Binding; D020798:Two-Hybrid System Techniques
D029681:Arabidopsis Proteins