Novel dialkoxytriazine-type glycosyl donors for cellulase-catalysed lactosylation.
Novel glycosidic compounds, 4,6-dialkoxy-1,3,5-triazin-2-yl β-lactosides (DAT-β-Lac), have been prepared directly in water from lactose. The reaction was carried out on a laboratory scale without protecting the hydroxy groups of lactose. The resulting triazine derivatives were found to be recognized by endo-β1,4-glucanase III from Trichoderma reesei (EGIII). The EGIII-catalysed transglycosylation of 4,6-dimethoxy-1,3,5-triazine derivative (DMT-β-Lac) with various glycosyl acceptors has successfully been demonstrated, affording the corresponding lactosylated products.
Tanaka
Tomonari
T
Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University, 6-6-11-514 Aoba, Sendai, Miyagi 980-8579, Japan.
Noguchi
Masato
M
Watanabe
Kazuhito
K
Misawa
Takuya
T
Ishihara
Masaki
M
Kobayashi
Atsushi
A
Shoda
Shin-Ichiro
S
D055162:Biocatalysis; D002236:Carbohydrate Conformation; D002480:Cellulase / Q000378:metabolism*; D006027:Glycosides / Q000737:chemistry / Q000378:metabolism*; D006031:Glycosylation; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007785:Lactose / Q000737:chemistry / Q000378:metabolism*; D014227:Triazines / Q000737:chemistry / Q000378:metabolism*; D014242:Trichoderma / Q000201:enzymology*
D006027:Glycosides; D014227:Triazines; D002480:Cellulase; D007785:Lactose