Hydrophilic interaction liquid chromatography-tandem mass spectrometry for drug development.
Hydrophilic interaction chromatography (HILIC) offers a difference in selectivity as compared to traditional normal phase and reversed-phase liquid chromatography and therefore has great potential for the separation of a variety of pharmaceuticals. This review is devoted to summarizing HILIC coupled to tandem mass spectrometric (MS/MS) methods for the rapid, sensitive and accurate determinations of small molecules employed for supporting drug discovery. The perspectives and challenges on performing HILIC-MS/MS assays are also presented.
Hsieh
Yunsheng
Y
Department of Drug Metabolism and Pharmacokinetics, Merck Research Laboratories, Kenilworth, NJ 07033, USA. yunsheng.hsieh@spcorp.com
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D055808:Drug Discovery; D057927:Hydrophobic and Hydrophilic Interactions; D004364:Pharmaceutical Preparations / Q000032:analysis*; D044367:Phase Transition; D053719:Tandem Mass Spectrometry / Q000295:instrumentation / Q000379:methods*
D004364:Pharmaceutical Preparations