title: Lanthanide-based luminescent probes for selective time-gated detection of hydrogen peroxide in water and in living cells.
abstract: Lanthanide-based luminescent probes TPR1 and TPR2 were developed for the detection of hydrogen peroxide (H(2)O(2)) in living systems. The chemoselective reaction of these boronate-protected probes with H(2)O(2) resulted in an enhanced lanthanide sensitization and a 6-fold increase in luminescent intensity. TPR2 was utilized to measure the endogenous production of H(2)O(2) in RAW 264.7 macrophages using time-gated luminescent spectroscopy.
authors.affiliation: Department of Chemistry and the Howard Hughes Medical Institute, University of California, Berkeley, CA 94720, USA.
authors.forename: Alexander R
authors.initials: AR
authors.lastname: Lippert
authors.forename: Tina
authors.initials: T
authors.lastname: Gschneidtner
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Chang
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods; D002460:Cell Line; D006861:Hydrogen Peroxide / Q000032:analysis / Q000378:metabolism*; D028581:Lanthanoid Series Elements / Q000737:chemistry*; D049408:Luminescent Agents / Q000737:chemistry*; D008163:Luminescent Measurements / Q000379:methods*; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D014867:Water / Q000737:chemistry
chemical_list: D028581:Lanthanoid Series Elements; D049408:Luminescent Agents; D014867:Water; D006861:Hydrogen Peroxide