The NC1 domain of type XIX collagen inhibits melanoma cell migration.
Type XIX collagen is a minor collagen that localizes to basement membrane zones. We previously demonstrated that the C-terminal NC1 domain of type XIX collagen inhibits tumor growth in vivo. In the present study, we analyzed the effects of the NC1(XIX) collagen domain on migratory behaviour of melanoma B16F10 cells. We found that NC1(XIX) do not inhibit melanoma cell proliferation. On the contrary, NC1(XIX) strongly inhibited the migratory capacities of melanoma cells in the scratch wound model and in Ibidi® devices: cell migration speed was 7.69 ± 1.49 μm/h for the controls vs 6.64 ± 0.82 μm/h for cells incubated with 30 μmol/L NC1(XIX) and 5.72 ± 0.67 μmol/h with 60 μmol/L NC1(XIX). Similar results were obtained with UACC 903 human melanoma cells. Further work will be necessary to elucidate the molecular mechanisms of this migration inhibition. It may, however, explain, at least partially, the inhibition of tumor growth that we observed in vivo.
Toubal
Aida
A
Université de Reims Champagne-Ardenne, 51095 Reims, France.
Ramont
Laurent
L
Terryn
Christine
C
Brassart-Pasco
Sylvie
S
Patigny
Dominique
D
Sapi
Janos
J
Monboisse
Jean-Claude
JC
Maquart
François-Xavier
FX
D000818:Animals; D001485:Basement Membrane / Q000378:metabolism; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation; D003094:Collagen / Q000494:pharmacology*; D005109:Extracellular Matrix / Q000378:metabolism; D008545:Melanoma / Q000473:pathology / Q000517:prevention & control*; D051379:Mice; D008810:Mice, Inbred C57BL; D009389:Neovascularization, Pathologic; D010446:Peptide Fragments / Q000378:metabolism; D012878:Skin Neoplasms / Q000473:pathology / Q000517:prevention & control*
D010446:Peptide Fragments; D003094:Collagen