[Association between interferon regulatory factor 6 gene polymorphism and non-syndromic oral clefting].
OBJECTIVE
To investigate the association between interferon regulatory factor 6 (IRF6) gene polymorphism and non-syndromic oral clefting (NSOC).


METHODS
Experimental group consisted of 186 Ningxia NSOC patients, their parents (183 fathers and 174 mothers), 172 core families (patient+parents), and control group consisted of 200 normal children. DNA was extracted and PCR-restriction fragment length polymorphism (PCR-RFLP) was used to identify the genotypes of the samples, case-control analyses and transmission-disequilibrium test (TDT) were carried out.


RESULTS
Compared with control group, there were significant differences in both rs642961's and rs4844880's AA genotype and A allele among NSOC patients (P < 0.05), but no difference in cleft palate (P = 0.15, P = 0.967, respectively). In TDT analysis, the A allele of rs642961 had a strong over-transmission in NSOC (P < 0.05), so did the rs4844880'A allele (P < 0.05), but neither of them had significant difference in cleft palate (P = 0.91, P = 0.95, respectively).


CONCLUSIONS
IRF6 gene polymorphism is associated with NSOC.
Ren
Hong-wang
HW
Dept. of Oral and Maxillofacial Surgery, College of Stomatology, Ningxia Medical University, Yinchuan 750004, China.
Zhao
Gui-zhi
GZ
Huang
Yong-qing
YQ
Ma
Min
M
Ma
Jian
J
Sun
Yan-bo
YB
Gao
Jun
J
Shi
Bing
B
D016022:Case-Control Studies; D002972:Cleft Palate / Q000235:genetics*; D006801:Humans; D050835:Interferon Regulatory Factors / Q000235:genetics*; D011110:Polymorphism, Genetic
C109803:IRF6 protein, human; D050835:Interferon Regulatory Factors