Alleviation of copper-induced oxidative damage in Chlamydomonas reinhardtii by carbon monoxide.
Carbon monoxide (CO) is an endogenous gaseous molecule in plants and animals. Recent studies have shown that it is one of the most essential cellular components regulating many aspects of plant growth and development. However, whether CO regulates the green algae adaptive response to heavy metal toxicity is unknown. The present study investigated the role of CO in regulating Cu-induced oxidative stress in eukaryotic algae Chlamydomonas reinhardtii. Cells pretreated with 5 μM CO for 30 min and followed by exposure to 5 μM Cu(II) for 4 days showed attenuated toxicity. The CO-improved growth of algae was correlated with reduced lipid peroxidation and increased chlorophyll accumulation. The beneficial effect of CO was confirmed by histochemical staining with reactive oxygen species. Further, treatment with 5 μM CO increased the activity of catalase with Cu. However, a reduced superoxide dismutase activity was observed in the CO + Cu-treated algae compared to the control (activity of Cu treatment alone). Under the same condition, the activity of ascorbate peroxidase was not significantly changed. These results suggest that CO can play an important role in regulating the response of algae to Cu stress.
Zheng
Qi
Q
Department of Biochemistry and Molecular Biology, College of Life Science, Nanjing Agricultural University, Nanjing, China.
Meng
Qian
Q
Wei
Yuan Yuan
YY
Yang
Zhi Min
ZM
D002248:Carbon Monoxide / Q000378:metabolism*; D016825:Chlamydomonas reinhardtii / Q000187:drug effects* / Q000378:metabolism; D002734:Chlorophyll / Q000378:metabolism; D003300:Copper / Q000633:toxicity*; D004591:Electrophoresis, Polyacrylamide Gel; D015227:Lipid Peroxidation; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism
D017382:Reactive Oxygen Species; D002734:Chlorophyll; D003300:Copper; D002248:Carbon Monoxide; D013482:Superoxide Dismutase