Efficient receptor-independent intracellular translocation of aptamers mediated by conjugation to carbon nanotubes.
We have covalently grafted aptamers onto carboxylated carbon nanotubes to design a novel vector system that can easily translocate into the cytosol of different cell types independent of receptor-mediated uptake. We propose the use of carbon nanotubes for the efficient intracellular delivery of biologically active aptamers for potential therapeutic applications.
Van den Bossche
Jeroen
J
Nanomedicine Laboratory, Centre for Drug Delivery, The School of Pharmacy, University of London, 29-39 Brunswick Square, London, UK WC1N 1AX.
Al-Jamal
Wafa' T
WT
Tian
Bowen
B
Nunes
Antonio
A
Fabbro
Chiara
C
Bianco
Alberto
A
Prato
Maurizio
M
Kostarelos
Kostas
K
D052157:Aptamers, Nucleotide; D001483:Base Sequence; D001692:Biological Transport; D045744:Cell Line, Tumor; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D046529:Microscopy, Electron, Transmission; D037742:Nanotubes, Carbon; D011956:Receptors, Cell Surface / Q000502:physiology*
D052157:Aptamers, Nucleotide; D017931:DNA Primers; D037742:Nanotubes, Carbon; D011956:Receptors, Cell Surface