title: qPCR assays to quantify genes and gene expression associated with microbial perchlorate reduction.
abstract: Quantitative PCR (qPCR) assays targeting cld (developed in this work) and pcrA (previously described) were used to quantify these perchlorate-related genes in a perchlorate-reducing enrichment culture. Transcript copies were quantified in perchlorate-reducing Rhodocyclaceae strain JDS4. Oxygen and nitrate inhibited expression of cld and pcrA.
authors.affiliation: The University of Texas at Austin, Department of Civil, Architectural, and Environmental Engineering, 1 University Station C1786, Austin, TX 78712, United States. Susan.De_Long@colostate.edu
authors.forename: Susan K
authors.initials: SK
authors.lastname: De Long
authors.forename: Kerry A
authors.initials: KA
authors.lastname: Kinney
authors.forename: Mary Jo
authors.initials: MJ
authors.lastname: Kirisits
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics; D004265:DNA Helicases / Q000096:biosynthesis / Q000235:genetics; D020869:Gene Expression Profiling / Q000379:methods*; D009566:Nitrates / Q000378:metabolism; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism; D010472:Perchlorates / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000379:methods*; D043362:Rhodocyclaceae / Q000235:genetics* / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D009566:Nitrates; D010472:Perchlorates; C084217:pcrA protein, Bacteria; C075969:rol protein, Bacteria; D004265:DNA Helicases; D010100:Oxygen; C494474:perchlorate