Capillary electrophoresis with noncovalently bilayer-coated capillaries for stability study of allergenic proteins in simulated gastrointestinal fluids.
A novel noncovalently bilayer-coated capillary using cationic polymer polybrene (PB) and anionic polymer (sodium 4-styrenesulfonate) (PSS) as coatings was prepared. This PB-PSS coating showed good migration-time reproducibility for proteins and high stability in the range of pH 2-10 and in the presence of 1M NaOH, acetonitrile and methanol. Capillary electrophoresis with PB-PSS coated capillaries was successfully applied to quantitatively investigate the stability of bovine serum albumin, ovomucoid, β-lactoglobulin and lysozyme in simulated gastrointestinal fluids. β-lactoglobulin A and β-lactoglobulin B were both stable in simulated gastric fluid with degradation percentages of 34.3% and 17.2% after 60min of incubation, respectively. Bovine serum albumin, ovomucoid and lysozyme were stable in simulated intestinal fluid with degradation percentages of 17.7%, 23.4% and 22.8% after 60min of incubation, respectively. The superiority of the proposed method over sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and capillary electrophoresis with untreated fused silica capillaries was demonstrated and emphasized.
Zheng
Chang
C
School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang 110016, PR China.
Liu
Youping
Y
Zhou
Qiuhong
Q
Di
Xin
X
D000485:Allergens / Q000737:chemistry* / Q000378:metabolism; D000818:Animals; D001826:Body Fluids / Q000737:chemistry*; D002417:Cattle; D004063:Digestion; D019075:Electrophoresis, Capillary / Q000379:methods*; D005512:Food Hypersensitivity; D041981:Gastrointestinal Tract / Q000737:chemistry*; D006583:Hexadimethrine Bromide / Q000737:chemistry; D007782:Lactoglobulins / Q000737:chemistry / Q000378:metabolism; D008954:Models, Biological; D009113:Muramidase / Q000737:chemistry / Q000378:metabolism; D010059:Ovomucin / Q000737:chemistry / Q000378:metabolism; D011108:Polymers / Q000737:chemistry; D055550:Protein Stability; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism; D015203:Reproducibility of Results; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism; D013451:Sulfonic Acids / Q000737:chemistry
D000485:Allergens; D007782:Lactoglobulins; D011108:Polymers; D011506:Proteins; D013451:Sulfonic Acids; D012710:Serum Albumin, Bovine; D010059:Ovomucin; D006583:Hexadimethrine Bromide; D009113:Muramidase; C077114:styrenesulfonic acid polymer