[Sequence the complete sequence of bocavirus I with SISPA-PCR].
OBJECTIVE
To sequence the complete sequence of bocavirus I with sequence independent single primer amplification (SISPA-PCR).


METHODS
To exclude the co-effection samples, all clinical samples of diarrhea cases were screened with special primers of rotavirus, astrovirus, adenovirus, calicivirus and bocavirus I. The virus were enriched through ultracentrifugation. Other nucleic acids, such as human and bacteria genomes, were degradated by DNase I and RNase. DNA of bocavirus was Amplificated with SISPA-PCR, then purificated, cloned and sequenced. The sequences were alighmented in nr with blastn and assembled with DNAstar.


RESULTS
A 4834bp sequence of bocavirus I were assembled.


CONCLUSIONS
SISPA-PCR is an economical and efficient technique for sequence a virus complete genome.
Li
Jin-Song
JS
Chinese Center for Disease Control and Prevention, Beijing 100052, China.
Cheng
Wei-Xia
WX
Yao
Dong-Ping
DP
Lan
Bei
B
Yu
Jie-Mei
JM
Liu
Yan
Y
Li
Yong-Qing
YQ
Zhang
Qing
Q
Jin
Miao
M
Xu
Zi-Qian
ZQ
Li
Dan-Di
DD
Duan
Zhao-Jun
ZJ
D001483:Base Sequence; D052660:Bocavirus / Q000235:genetics* / Q000302:isolation & purification; D017931:DNA Primers / Q000235:genetics*; D003967:Diarrhea / Q000821:virology*; D016679:Genome, Viral; D006801:Humans; D010322:Parvoviridae Infections / Q000821:virology*; D016133:Polymerase Chain Reaction
D017931:DNA Primers