Photoelectronic characterization of IgG antibody molecule-quantum dot hybrid as biosensing probe.
Quantum dot (QD)-based biomolecule hybrids have recently attracted much attention in specifically identifying and labeling target proteins. In this study, QD encapsulated with immunoglobulin antibodies, as a labeling building block in biosensors, was investigated to clarify the most efficient configuration and photoluminescence behavior. Both the biological recognition capacity and photoluminescence emitting signal of the antibody-coupled nanocrystal were validated through a photoelectrical characterization procedure. Derivation of the optimum number of antibody molecules to be packed onto the QD surface yielded the highest binding capacity for the target antigen. During formation of the bioactive layer, the intrinsic photoluminescence response of the QDs significantly decreased due to photoinduced hole transfer according to their rearranged electronic structure. The thorough study of this assembly provides a validation approach for the careful titration of biosensor probes for optimal reaction kinetics. Furthermore, it contributes to the development of an effective tool for the application and interpretation of QD-based labeling techniques.
Yu
Hye-Weon
HW
School of Environmental Science and Engineering, Gwangju Institute of Science and Technology (GIST), Buk-gu, Gwangju, Republic of Korea.
Lee
Jinwook
J
Kim
Sungyoun
S
Kim
Sung-Jo
SJ
Kim
In S
IS
D000941:Antigens / Q000276:immunology; D001427:Bacterial Toxins / Q000276:immunology; D015374:Biosensing Techniques / Q000379:methods*; D000458:Cyanobacteria / Q000276:immunology; D007074:Immunoglobulin G / Q000737:chemistry* / Q000276:immunology*; D049449:Luminescence; D052998:Microcystins / Q000276:immunology; D053758:Nanoparticles / Q000737:chemistry; D010777:Photochemistry; D045663:Quantum Dots
D000941:Antigens; D001427:Bacterial Toxins; D007074:Immunoglobulin G; D052998:Microcystins; C078588:microcystin