Genome structures and halophyte-specific gene expression of the extremophile Thellungiella parvula in comparison with Thellungiella salsuginea (Thellungiella halophila) and Arabidopsis.
The genome of Thellungiella parvula, a halophytic relative of Arabidopsis (Arabidopsis thaliana), is being assembled using Roche-454 sequencing. Analyses of a 10-Mb scaffold revealed synteny with Arabidopsis, with recombination and inversion and an uneven distribution of repeat sequences. T. parvula genome structure and DNA sequences were compared with orthologous regions from Arabidopsis and publicly available bacterial artificial chromosome sequences from Thellungiella salsuginea (previously Thellungiella halophila). The three-way comparison of sequences, from one abiotic stress-sensitive species and two tolerant species, revealed extensive sequence conservation and microcolinearity, but grouping Thellungiella species separately from Arabidopsis. However, the T. parvula segments are distinguished from their T. salsuginea counterparts by a pronounced paucity of repeat sequences, resulting in a 30% shorter DNA segment with essentially the same gene content in T. parvula. Among the genes is SALT OVERLY SENSITIVE1 (SOS1), a sodium/proton antiporter, which represents an essential component of plant salinity stress tolerance. Although the SOS1 coding region is highly conserved among all three species, the promoter regions show conservation only between the two Thellungiella species. Comparative transcript analyses revealed higher levels of basal as well as salt-induced SOS1 expression in both Thellungiella species as compared with Arabidopsis. The Thellungiella species and other halophytes share conserved pyrimidine-rich 5' untranslated region proximal regions of SOS1 that are missing in Arabidopsis. Completion of the genome structure of T. parvula is expected to highlight distinctive genetic elements underlying the extremophile lifestyle of this species.
Oh
Dong-Ha
DH
Department of Plant Biology , University of Illinois, Urbana, Illinois 61801, USA. ohdongha@life.illinois.edu
Dassanayake
Maheshi
M
Haas
Jeffrey S
JS
Kropornika
Anna
A
Wright
Chris
C
d'Urzo
Matilde Paino
MP
Hong
Hyewon
H
Ali
Shahjahan
S
Hernandez
Alvaro
A
Lambert
Georgina M
GM
Inan
Gunsu
G
Galbraith
David W
DW
Bressan
Ray A
RA
Yun
Dae-Jin
DJ
Zhu
Jian-Kang
JK
Cheeseman
John M
JM
Bohnert
Hans J
HJ
D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins; D019607:Brassicaceae / Q000235:genetics*; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D018745:Genome, Plant; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D055051:Salt-Tolerant Plants / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017923:Sodium-Hydrogen Exchangers / Q000235:genetics
D029681:Arabidopsis Proteins; D018744:DNA, Plant; C477653:SOS1 protein, Arabidopsis; D017923:Sodium-Hydrogen Exchangers