title: Role of Rho-ROCK signaling in MOLT4 cells metastasis induced by CCL25.
abstract: Our previous research has revealed that binding of the chemokine CCL25 to the CCR9 provides chemotactic cues guiding leukemic cells to specific tissues and organs. The RhoA-ROCK pathway might be involved in cancer migration. The purpose of this study was to explore the role of the RhoA-ROCK-MLC axis in leukemic cell migration following exposure to CCL25. The results showed that CCL25 could increase the amount of the GTPase RhoA and activate MLC in MOLT4 cells in a time-dependent manner. C3 exoenzyme and Y-27632 could block MOLT4 cell migration and chemotaxis. Thus, the RhoA-ROCK-MLC axis might play an important role in MOLT4 cell metastasis induced by CCL25.
authors.affiliation: Department of Immunology, College of Basic Medical Science, Wuhan University, 185 Donghu Road, Wuhan, 430071 Hubei, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Beibei
authors.initials: B
authors.lastname: Yu
authors.forename: Meng
authors.initials: M
authors.lastname: Hu
authors.forename: Zhan
authors.initials: Z
authors.lastname: Wang
authors.forename: Dongying
authors.initials: D
authors.lastname: Liu
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Tong
authors.forename: Jun
authors.initials: J
authors.lastname: Leng
authors.forename: Beibei
authors.initials: B
authors.lastname: Zhou
authors.forename: Yi
authors.initials: Y
authors.lastname: Hu
authors.forename: Ranran
authors.initials: R
authors.lastname: Wu
authors.forename: Qianshan
authors.initials: Q
authors.lastname: Ding
authors.forename: Qiuping
authors.initials: Q
authors.lastname: Zhang
mesh_terms: D002460:Cell Line; D019742:Chemokines, CC / Q000502:physiology*; D006801:Humans; D009362:Neoplasm Metastasis; D054460:rho-Associated Kinases / Q000378:metabolism*; D020742:rhoA GTP-Binding Protein / Q000378:metabolism*
chemical_list: C108074:CCL25 protein, human; D019742:Chemokines, CC; C515851:ROCK1 protein, human; D054460:rho-Associated Kinases; D020742:rhoA GTP-Binding Protein