Insights into enzyme kinetics of chloroethane biodegradation using compound specific stable isotopes.
While compound specific isotope analysis (CSIA) has been used extensively to investigate remediation of chlorinated ethenes, to date considerably less information is available on its applicability to chlorinated ethanes. In this study, biodegradation of 1,1,1-trichloroethane (1,1,1-TCA) and 1,1-dichloroethane (1,1-DCA) was carried out by a Dehalobacter-containing mixed culture. Carbon isotope fractionation factors (ε) measured during whole cell degradation demonstrated that values for 1,1,1-TCA and 1,1-DCA (-1.8‰ and -10.5‰, respectively) were significantly smaller than values reported for abiotic reductive dechlorination of these same compounds. Similar results were found in experiments degrading these two priority pollutants by cell free extracts (CFE) where values of -0.8‰ and -7.9‰, respectively, were observed. For 1,1,1-TCA in particular, the large kinetic isotope effect expected for cleavage of a C-Cl bond was almost completely masked during biodegradation by both whole cells and CFE. Comparison to previous studies demonstrates that these patterns of isotopic fractionation are not attributable to transport effects across the cell membrane, as had been seen for other compounds such as PCE. In contrast these results reflect significant differences in the kinetics of the enzymes catalyzing chlorinated ethane degradation.
Lollar
Barbara Sherwood
BS
Department of Geology, University of Toronto, Toronto, Ontario, Canada. bslollar@chem.utoronto.ca
Hirschorn
Sarah
S
Mundle
Scott O C
SO
Grostern
Ariel
A
Edwards
Elizabeth A
EA
Lacrampe-Couloume
Georges
G
D001673:Biodegradation, Environmental; D007554:Isotopes; D007700:Kinetics; D008737:Methyl Chloride / Q000378:metabolism*; D010459:Peptococcaceae / Q000378:metabolism*; D015203:Reproducibility of Results
D007554:Isotopes; D008737:Methyl Chloride