[Optimization of the extraction technology of total flavonoids from Mulgedium tataricum using response surface analysis].
OBJECTIVE
Response surface analysis methodology (RSM) was used to optimize the extraction technology of total flavonoids from Mulgedium tataricum.


METHODS
Ethanol concentration, solvent-solid ratio, extraction temperature and extraction time were selected as influencing factors during extraction. The experiment mathematical model was arranged according to Box-Behnken central composite experiment design.


RESULTS
The optimal extraction conditions were as following: ethanol concentration 63.78%, solvent-solid ratio 40: 1, extraction temperature 90 degrees C, and extraction time 1.07 h, the predicted value of extraction rate of total flavonoids was 14.04% under the optimum conditions, and the experimental value was 13.79%.


CONCLUSIONS
The combination of Box-Behnken design and response surface analysis can well optimize the extraction technology of total flavonoids from Mulgedium tataricum.
Gao
Yi-Xia
YX
College of Life Science and Chemistry, Tian shui Normal University, Tianshui 741001, China. egaoxy@126.com
Jing
Hong-Yan
HY
Jiang
Zu-Jun
ZJ
Zhang
Ji
J
Yang
Sheng
S
D000704:Analysis of Variance; D019659:Asteraceae / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification; D000431:Ethanol / Q000737:chemistry; D005419:Flavonoids / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D008962:Models, Theoretical; D012997:Solvents; D013678:Technology, Pharmaceutical / Q000379:methods*; D013696:Temperature; D013997:Time Factors
D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D012997:Solvents; D000431:Ethanol