title: CAPN1 markers in three Argentinean cattle breeds: report of a new InDel polymorphism within intron 17.
abstract: In this study, the genotype distribution and allelic frequencies of CAPN1 (Calcium activated neutral protease) single nucleotide polymorphisms (SNPs) were analyzed taking advantage of the different genetic backgrounds provided by Hereford, Brahman and Braford cattle. We report a new insertion/deletion (InDel) polymorphism, consisting of a change of seven nucleotides for only one nucleotide (TCTGGGT → C) within intron 17 of the CAPN1 gene. The segregation pattern of this polymorphism was analyzed together with the markers CAPN316, CAPN530 and CAPN4751 already described. The allele distribution of CAPN1 markers in the Braford crossbreed (3/8 Brahman 5/8 Hereford) is described for the first time. Four assays of allelic discrimination were designed: the tetra primer ARMS-PCR technique for genotyping the new InDel and the CAPN4751 marker, and a PCR-RFLP method for genotyping the markers CAPN316 and CAPN530. The genotypic and minor allele frequencies (MAFs) obtained showed that the InDel polymorphism does not provide redundant information to that already provided by the other CAPN1 markers and segregates differently between breeds, being a common SNP (MAF ≥ 0.05) in the herds with a high percentage of Bos indicus background. The high percentage of heterozygous individuals found in the Braford crossbreed for the markers assessed reveals enough genetic variation that could help to solve the tenderness problem of tropical-adapted cattle.
authors.affiliation: Instituto de Genética Ewald A Favret, CICVyA-INTA, CC 25 (1712), Castelar, Buenos Aires, Argentina. patricia.p.iglesias@gmail.com
authors.forename: P P
authors.initials: PP
authors.lastname: Iglesias
authors.forename: M E
authors.initials: ME
authors.lastname: Caffaro
authors.forename: A F
authors.initials: AF
authors.lastname: Amadio
authors.forename: A
authors.initials: A
authors.lastname: Arias Mañotti
authors.forename: M A
authors.initials: MA
authors.lastname: Poli
mesh_terms: D000818:Animals; D001118:Argentina; D001947:Breeding; D002154:Calpain / Q000235:genetics*; D002417:Cattle / Q000235:genetics*; D005787:Gene Frequency / Q000235:genetics; D005819:Genetic Markers; D054643:INDEL Mutation / Q000235:genetics*; D007438:Introns / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D005819:Genetic Markers; D002154:Calpain; C484807:CAPN1 protein, Bos taurus