Inhibitory components from the buds of clove (Syzygium aromaticum) on melanin formation in B16 melanoma cells.
In the course to find a new whitening agent, we evaluated the methanol extract from bud of clove (Syzygium aromaticum) on melanin formation in B16 melanoma cells. Eugenol and eugenol acetate were isolated as the active compounds and showed melanin inhibition of 60% and 40% in B16 melanoma cell with less cytotoxicity at the concentration of 100 and 200 μg/mL, respectively. Furthermore, an essential oil prepared from the bud of clove, which contain eugenol and eugenol acetate as dominant components, showed melanin inhibition of 50% and 80% in B16 melanoma cells at the concentration of 100 and 200 μg/mL, respectively.
Arung
Enos Tangke
ET
Department of Agro-environmental Sciences, Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan.
Matsubara
Eri
E
Kusuma
Irawan Wijaya
IW
Sukaton
Edi
E
Shimizu
Kuniyoshi
K
Kondo
Ryuichiro
R
D000085:Acetates / Q000302:isolation & purification / Q000494:pharmacology; D000818:Animals; D045744:Cell Line, Tumor; D003879:Dermatologic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D005054:Eugenol / Q000302:isolation & purification / Q000494:pharmacology*; D035264:Flowers; D008543:Melanins / Q000037:antagonists & inhibitors*; D008546:Melanoma, Experimental / Q000378:metabolism*; D051379:Mice; D009822:Oils, Volatile / Q000737:chemistry / Q000494:pharmacology*; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D027842:Syzygium / Q000737:chemistry*
D000085:Acetates; D003879:Dermatologic Agents; D008543:Melanins; D009822:Oils, Volatile; D010936:Plant Extracts; D005054:Eugenol