Advanced glycation end products in diabetic and non-diabetic human subjects suffering from cataract.
Advanced glycation end products (AGEs) play a pivotal role in loss of lens transparency, i.e., cataract. AGEs formation occurs as a result of sequential glycation and oxidation reaction between reducing sugars and protein. AGEs production takes place throughout the normal aging process but its accumulation is found to be more rapid in diabetic patients. In this study, we quantified AGEs and N-(carboxyethyl) lysine (CEL) in human cataractous lenses from non-diabetic (n=50) and diabetic patients (n=50) using ELISA. We observed significantly higher (p<0.001) levels of lens AGEs and CEL in diabetic patients with cataract as compared with their respective controls. The presence of AGEs and CEL was also determined by western blotting and immuno-histochemical analysis. Furthermore, isolated β-crystallin from cataractous lenses of non-diabetic and diabetic patients was incubated with different sugars to evaluate the extent of glycation in a time dependent manner. Our data indicated more pronounced glycation in patients suffering from diabetes as compared to non-diabetics subjects demonstrating the need to focus on developing normoglycemic approaches. Such studies may provide an insight in developing therapeutic strategies and may have clinical implications.
Hashim
Zehra
Z
National Center for Proteomics, University of Karachi, Karachi, Pakistan.
Zarina
Shamshad
S
D000368:Aged; D002386:Cataract / Q000209:etiology / Q000378:metabolism*; D003924:Diabetes Mellitus, Type 2 / Q000150:complications / Q000378:metabolism*; D005260:Female; D017127:Glycation End Products, Advanced / Q000009:adverse effects* / Q000032:analysis / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D007908:Lens, Crystalline / Q000737:chemistry; D008239:Lysine / Q000009:adverse effects / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D038204:beta-Crystallins / Q000378:metabolism*
D017127:Glycation End Products, Advanced; D038204:beta-Crystallins; C054688:N(6)-(1-carboxyethyl)lysine; D008239:Lysine