MIQE précis: Practical implementation of minimum standard guidelines for fluorescence-based quantitative real-time PCR experiments.
The conclusions of thousands of peer-reviewed publications rely on data obtained using fluorescence-based quantitative real-time PCR technology. However, the inadequate reporting of experimental detail, combined with the frequent use of flawed protocols is leading to the publication of papers that may not be technically appropriate. We take the view that this problem requires the delineation of a more transparent and comprehensive reporting policy from scientific journals. This editorial aims to provide practical guidance for the incorporation of absolute minimum standards encompassing the key assay parameters for accurate design, documentation and reporting of qPCR experiments (MIQE précis) and guidance on the publication of pure 'reference gene' articles.
Bustin
Stephen A
SA
Beaulieu
Jean-François
JF
Huggett
Jim
J
Jaggi
Rolf
R
Kibenge
Frederick S B
FS
Olsvik
Pål A
PA
Penning
Louis C
LC
Toegel
Stefan
S
D005453:Fluorescence; D017712:Peer Review, Research / Q000592:standards; D010506:Periodicals as Topic / Q000592:standards; D016133:Polymerase Chain Reaction / Q000295:instrumentation / Q000379:methods / Q000592:standards; D011643:Publishing / Q000592:standards