Autologous transplantation of ischemically injured kidneys in pigs.
BACKGROUND
Expansion of the organ donor pool can be obtained through novel interventions attenuating ischemic acute kidney injury, which will enable the use of kidneys that suffered prolonged ischemia. In basic science, new therapeutic targets are identified that should be tested in a relevant large animal model before use in human kidney transplantation.


METHODS
The current paper provides a detailed description of the technique of autologous transplantation of ischemically injured kidneys in pigs with special emphasis on perioperative care.


RESULTS
The animal model was validated by showing that renal function after transplantation was proportional to the duration of warm ischemia before organ recovery. The extent of renal dysfunction was reproducible following kidney transplantations with the same warm ischemia time.


CONCLUSIONS
Our experience may reduce the learning curves of other research groups taking an interest in the model and improve preclinical testing of novel interventions that modulate renal ischemia and reperfusion injury in kidney transplantation.
Snoeijs
Maarten G
MG
Department of Surgery, Maastricht University Medical Center, The Netherlands. m.snoeijs@mumc.nl
Matthijsen
Robert A
RA
Seeldrayers
Saskia
S
Marcus
Marco A
MA
Daemen
Jan-Willem H
JW
Peutz-Kootstra
Carine J
CJ
Buurman
Wim A
WA
Schurink
Geert Willem H
GW
Ernest van Heurn
L W
LW
D000208:Acute Disease; D000818:Animals; D004195:Disease Models, Animal; D006085:Graft Survival / Q000502:physiology*; D007668:Kidney / Q000098:blood supply / Q000502:physiology* / Q000601:surgery; D016030:Kidney Transplantation; D008297:Male; D020127:Recovery of Function / Q000502:physiology*; D012077:Renal Artery / Q000601:surgery; D015427:Reperfusion Injury / Q000473:pathology*; D034421:Sus scrofa; D013696:Temperature; D013997:Time Factors; D014182:Transplantation, Autologous