title: Is a single nucleotide polymorphism a risk factor for lung cancer in the matrix metalloproteinase-2 promoter?
abstract: We aimed to investigate the association of polymorphism frequencies of MMP-2 C1306T and MMP-2 plasma enzyme activity in lung cancer patients. In this study 300 genomic DNA (200 lung cancer patients + 100 no lung cancer) were analyzed. Polymorphisms were determined by using polymerase chain reaction-restriction fragment length polymorphism (RFLP) and electrophoresis. Plasma MMP-2 enzyme activity levels were measured by using ELISA. Sex, asbestos expose and smoking might be risk factors for lung cancer. The frequencies of C1306T genotypes in controls CC 65%, CT 32%, TT 3% and in patients CC 61%, CT 37%, TT2 % were found. It was determined that CC genotype frequency increase significantly in patients and controls. Plasma MMP-2 enzyme activity levels were increased in lung cancer patients according to controls. Finally, we claimed that determining of MMP-2 enzyme level can be used as a marker in lung cancer.
authors.affiliation: Faculty of Medicine, Department of Medical Biology, Eskisehir Osmangazi University, 26480 Eskisehir, Turkey. atbayramoglu@yahoo.com
authors.forename: Bayramoglu
authors.initials: B
authors.lastname: Ayşegül
authors.forename: Gunes Hasan
authors.initials: GH
authors.lastname: Veysi
authors.forename: Metintas
authors.initials: M
authors.lastname: Muzaffer
authors.forename: Degirmenci
authors.initials: D
authors.lastname: Irfan
authors.forename: Akın
authors.initials: A
authors.lastname: Azra
authors.forename: Kurt
authors.initials: K
authors.lastname: Hulyam
mesh_terms: D016022:Case-Control Studies; D005787:Gene Frequency / Q000235:genetics; D020022:Genetic Predisposition to Disease; D006801:Humans; D008175:Lung Neoplasms / Q000201:enzymology* / Q000235:genetics*; D020778:Matrix Metalloproteinase 2 / Q000097:blood / Q000235:genetics*; D009154:Mutation / Q000235:genetics; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012372:ROC Curve; D012307:Risk Factors
chemical_list: C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2