title: Specific protein content of pools of plasma for fractionation from different sources: impact of frequency of donations.
abstract: OBJECTIVE
  Plasma pools for the production of human plasma medicinal products are distinguished according to the collection method (recovered or apheresis plasma) and the donor remuneration status. National regulations and the physical status of the donor determine the donation frequency and plasma volume per session. Relevant protein contents of different types of pools have not fully been compared.
  
  
  METHODS
  We compared the levels of total protein, 15 main relevant plasma protein markers, and anti-B19 and anti-Streptococcus pneumoniae IgG in single-type pools of donations from different countries (Belgium, Finland, France, the Netherlands, Germany, United States). Both recovered plasma from non-remunerated donors and apheresis plasma from remunerated and non-remunerated donors were studied.
  
  
  RESULTS
  Pools from paid US high-frequency, high-volume plasmapheresis donors showed significantly lower total protein (-9%), albumin (-15%), total IgG (-24%), IgM (-28%), hemopexin (-11%) and retinol-binding protein (-10%) but higher C1-inhibitor, pre-albumin and C-reactive protein contents than pools from unpaid European Union (EU) or US whole-blood or plasmapheresis donors. In contrast to pools from compensated EU plasmapheresis donors, pools from unpaid whole-blood or plasmapheresis donors showed no significant differences, whatever the collection method or country. Reductions in specific protein contents correlated well with protein half-life.
  
  
  CONCLUSIONS
  These results should be taken into account with regard to donor health management and protein recovery.
authors.affiliation: Central Department for Fractionation, Red Cross, Brussels, Belgium. ruth.laub@caf-dcf-redcross.be
authors.forename: R
authors.initials: R
authors.lastname: Laub
authors.forename: S
authors.initials: S
authors.lastname: Baurin
authors.forename: D
authors.initials: D
authors.lastname: Timmerman
authors.forename: T
authors.initials: T
authors.lastname: Branckaert
authors.forename: P
authors.initials: P
authors.lastname: Strengers
mesh_terms: D000907:Antibodies, Bacterial / Q000097:blood / Q000737:chemistry; D000914:Antibodies, Viral / Q000032:analysis / Q000097:blood; D001782:Blood Donors; D001798:Blood Proteins / Q000032:analysis / Q000378:metabolism*; D005060:Europe; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis / Q000097:blood; D016732:Parvovirus B19, Human; D010949:Plasma / Q000737:chemistry / Q000378:metabolism*; D013296:Streptococcus pneumoniae; D014481:United States
chemical_list: D000907:Antibodies, Bacterial; D000914:Antibodies, Viral; D001798:Blood Proteins; D007074:Immunoglobulin G