[Effect of early high fat diet on pancreatic β cellularity and insulin sensibility in young rats].
OBJECTIVE
To study the effects of early high fat diet on sugar metaboliam, insulin sensibility and pancreatic β cellularity in young rats.


METHODS
Sixty male weaned young rats were randomly fed with high fat diet (high fat group) and normal diet (control group). The body weight, viscus fattiness and fasting plasma glucose (FPG) were measured after 3, 6 and 9 weeks. Serum insulin level was measured with radioimmunoassay. The ultrastructure of pancreas was observed under an electricmicroscope.


RESULTS
The high fat group had significantly higher body weight and visceral fat weight than the control group after 3 weeks. There were no significant differences in the FPG level between the two groups at all time points. The levels of fasting insulin and HOMAIR in the high fat group were significantly higher than those in the control group after 3, 6 and 9 weeks (P<0.01). Dilation of rough endoplasmic reticulum and mild swelling of mitochondria of islet β-cells were observed in the high fat group after 6 weeks.


CONCLUSIONS
Early high fat diet may induce a reduction in insulin sensitivity and produce insulin resistance in young rats. Endoplasmic reticulum expansion in β-cells may be an early sign of β-cell damage due to obesity.
Xie
Kun-Xia
KX
Department of Pediatrics, Xi'an Jiaotong Univesity, Xi'an 710004, China.
Xiao
Yan-Feng
YF
Xu
Er-Di
ED
Yin
Chun-Yan
CY
Yi
Xiao-Qing
XQ
Chang
Ming
M
D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D004041:Dietary Fats / Q000009:adverse effects*; D007328:Insulin; D007333:Insulin Resistance; D050417:Insulin-Secreting Cells / Q000473:pathology* / Q000648:ultrastructure; D050152:Intra-Abdominal Fat / Q000473:pathology; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
D001786:Blood Glucose; D004041:Dietary Fats; D007328:Insulin