title: Comparative study of the chondrogenic potential of human bone marrow stromal cells, neonatal chondrocytes and adult chondrocytes.
abstract: Cartilage tissue engineering is still a major clinical challenge with optimisation of a suitable source of cells for cartilage repair/regeneration not yet fully addressed. The aims of this study were to compare and contrast the differences in chondrogenic behaviour between human bone marrow stromal cells (HBMSCs), human neonatal and adult chondrocytes to further our understanding of chondroinduction relative to cell maturity and to identify factors that promote chondrogenesis and maintain functional homoeostasis. Cells were cultured in monolayer in either chondrogenic or basal medium, recapitulating procedures used in existing clinical procedures for cell-based therapies. Cell doubling time, morphology and alkaline phosphatase specific activity (ALPSA) were determined at different time points. Expression of chondrogenic markers (SOX9, ACAN and COL2A1) was compared via real time polymerase chain reaction. Amongst the three cell types studied, HBMSCs had the highest ALPSA in basal culture and lowest ALPSA in chondrogenic media. Neonatal chondrocytes were the most proliferative and adult chondrocytes had the lowest ALPSA in basal media. Gene expression analysis revealed a difference in the temporal expression of chondrogenic markers which were up regulated in chondrogenic medium compared to levels in basal medium. Of the three cell types studied, adult chondrocytes offer a more promising cell source for cartilage tissue engineering. This comparative study revealed differences between the microenvironment of all three cell types and provides useful information to inform cell-based therapies for cartilage regeneration.
authors.affiliation: Biomaterials and Tissue Engineering Group, Leeds Dental Institute, University of Leeds, LS29LU, UK.
authors.forename: Sushmita
authors.initials: S
authors.lastname: Saha
authors.forename: Jennifer
authors.initials: J
authors.lastname: Kirkham
authors.forename: David
authors.initials: D
authors.lastname: Wood
authors.forename: Stephen
authors.initials: S
authors.lastname: Curran
authors.forename: Xuebin
authors.initials: X
authors.lastname: Yang
mesh_terms: D000328:Adult; D053674:Aggrecans / Q000235:genetics; D001854:Bone Marrow Cells / Q000166:cytology / Q000502:physiology*; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology / Q000502:physiology*; D024043:Collagen Type II / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D055758:SOX9 Transcription Factor / Q000235:genetics; D017154:Stromal Cells / Q000166:cytology / Q000502:physiology; D023822:Tissue Engineering
chemical_list: C508319:ACAN protein, human; D053674:Aggrecans; C499177:COL2A1 protein, human; D024043:Collagen Type II; D055758:SOX9 Transcription Factor; C527932:SOX9 protein, human