Calcium and iron regulate swarming and type III secretion in Vibrio parahaemolyticus.
Here, we probe the response to calcium during growth on a surface and show that calcium influences the transcriptome and stimulates motility and virulence of Vibrio parahaemolyticus. Swarming (but not swimming) gene expression and motility were enhanced by calcium. Calcium also elevated transcription of one of the organism's two type III secretion systems (T3SS1 but not T3SS2) and heightened cytotoxicity toward host cells in coculture. Calcium stimulation of T3SS gene expression has not been reported before, although low calcium is an inducing signal for the T3SS of many organisms. EGTA was also found to increase T3SS1 gene expression and virulence; however, this was demonstrated to be the consequence of iron rather than calcium chelation. Ectopic expression of exsA, encoding the T3SS1 AraC-type regulator, was used to define the extent of the T3SS1 regulon and verify its coincident induction by calcium and EGTA. To begin to understand the regulatory mechanisms modulating the calcium response, a calcium-repressed, LysR-type transcription factor named CalR was identified and shown to repress swarming and T3SS1 gene expression. Swarming and T3SS1 gene expression were also demonstrated to be linked by LafK, a σ(54)-dependent regulator of swarming, and additionally connected by a negative-feedback loop on the swarming regulon propagated by ExsA. Thus, calcium and iron, two ions pertinent for a marine organism and pathogen, play a signaling role with global consequences on the regulation of gene sets that are relevant for surface colonization and infection.
Gode-Potratz
Cindy J
CJ
Microbiology Department, The University of Iowa, Iowa City, IA 52242, USA.
Chodur
Daniel M
DM
McCarter
Linda L
LL
D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism*; D016466:CHO Cells; D002118:Calcium / Q000378:metabolism*; D002470:Cell Survival; D018920:Coculture Techniques; D006224:Cricetinae; D003412:Cricetulus; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D007501:Iron / Q000378:metabolism*; D008124:Locomotion / Q000187:drug effects*; D026901:Membrane Transport Proteins / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D021381:Protein Transport / Q000187:drug effects; D014158:Transcription, Genetic / Q000187:drug effects; D014736:Vibrio parahaemolyticus / Q000187:drug effects* / Q000378:metabolism / Q000472:pathogenicity / Q000502:physiology*; D014774:Virulence
D001426:Bacterial Proteins; D026901:Membrane Transport Proteins; D007501:Iron; D002118:Calcium