The expression of PTHLH in human gastric mucosa enterochromaffin-like cells.
BACKGROUND
Gastrectomy may disturb the body's mineral homeostasis, with osteopenia and osteoporosis being among the late outcomes. Parathyroid hormone-like hormone (PTHLH) was detected in rat gastric enterochromaffin-like (ECL) cells in 2005, and some researchers suggested that it was the hypothetical hormone gastrocalcin that is believed to lead to osteoporosis.


OBJECTIVE
Our objective was to learn whether PTHLH is expressed in human gastric ECL cells and to form a basic understanding about the relationship between PTHLH and gastrin.


METHODS
We collected normal human gastric mucosa specimens and serum samples from 28 patients.


RESULTS
RT-PCR and immunohistochemical analysis demonstrated weak expression of PTHLH in ECL cells at the RNA and protein levels. A low level of PTHLH expression was also found in the serum. Serum gastrin did have a significant positive correlation with the relative ratio of PTHLH mRNA to β-actin levels in gastric mucosa (rs=0.569, p=0.002).


CONCLUSIONS
This indicates that PTHLH has a low signal expression in human gastric ECL cells and that serum gastrin levels correlate with PTHLH RNA levels in gastric mucosa. Further work is needed to evaluate the functional role of PTHLH in ECL cells and to determine whether PTHLH is gastrocalcin.
Liu
Cuiping
C
GuangDong Provincial Key Laboratory of Gastroenterology, Department for Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou Road 1838, 510515, Guangzhou, Guangdong Province, China. bullebell@163.com
Chen
Jianting
J
Guo
Yu
Y
Yang
Lisheng
L
Zhao
Chengyi
C
Bai
Lan
L
D000199:Actins / Q000378:metabolism; D000328:Adult; D000368:Aged; D019861:Enterochromaffin-like Cells / Q000378:metabolism*; D005260:Female; D005755:Gastrins / Q000097:blood*; D006801:Humans; D008297:Male; D008875:Middle Aged; D044162:Parathyroid Hormone-Related Protein / Q000096:biosynthesis* / Q000097:blood*; D013272:Stomach Diseases / Q000601:surgery
D000199:Actins; D005755:Gastrins; C470121:PTHLH protein, human; D044162:Parathyroid Hormone-Related Protein