title: Evaluation of the interaction between β-cyclodextrin and psychotropic drugs by surface plasmon resonance assay with a Biacore ® system.
abstract: Phase-solubility studies have been used to evaluate the solubilizing effects of cyclodextrins (CDs) on lipophilic, water-insoluble drugs. However, large amounts of CDs and drugs are required to measure solubility by phase-solubility studies. Thus, more efficient approaches to evaluate the interaction of CDs with drugs are needed. Herein we introduce a method that evaluates the interaction between immobilized β-cyclodextrin and psychotropic drugs by surface plasmon resonance assay with a Biacore(®) system. Association constants and stoichiometries observed were generally consistent with values calculated by traditional methods, such as phase-solubility and continuous variation methods. Results showed that the analytical method using Biacore(®) was suitable to evaluate CD-drug interactions.
authors.affiliation: Department of Physical Chemistry, Hoshi University, 4-41, Ebara 2-chome, Shinagawa-ku, Tokyo 142-8501, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Kobayashi
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Endo
authors.forename: Noriko
authors.initials: N
authors.lastname: Ogawa
authors.forename: Hiromasa
authors.initials: H
authors.lastname: Nagase
authors.forename: Mariko
authors.initials: M
authors.lastname: Iwata
authors.forename: Haruhisa
authors.initials: H
authors.lastname: Ueda
mesh_terms: D000657:Amoxapine / Q000737:chemistry; D002623:Chemistry Techniques, Analytical; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D006801:Humans; D007700:Kinetics; D008956:Models, Chemical; D011485:Protein Binding; D011619:Psychotropic Drugs / Q000737:chemistry*; D015203:Reproducibility of Results; D012995:Solubility; D020349:Surface Plasmon Resonance / Q000295:instrumentation / Q000379:methods*; D014196:Trazodone / Q000737:chemistry; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D011619:Psychotropic Drugs; D047392:beta-Cyclodextrins; C031215:betadex; D000657:Amoxapine; D014196:Trazodone