Structural characterization of an arabinogalactan from Platycodon grandiflorum roots and antiangiogenic activity of its sulfated derivative.
A water-soluble polysaccharide, PGAW1, with an average molecular mass of 9.2 kDa determined by high performance gel permeation chromatography (HPGPC), was isolated from radix of Platycodon grandiflorum . Monosaccharide composition analysis indicated that PGAW1 contains Ara and Gal in the molar ratio of 1.42:1.0. Using methylation analysis, partial hydrolysis, endo-1,4-β-d-galactanase digestion, NMR and ESI-MS, PGAW1 was determined to possess a backbone consisting of 1,4- and 1,6-linked galactopyranosyl residues, with branches attached to O-3 of 1,6-linked galactose residues. By the chlorosulfonic acid-pyridine method we produced a sulfated derivative of PGAW1, Sul-w1, with a substitution degree of 1.52. The substitution was at O-6 on 1,4-linked Gal residues according to the (13)C NMR spectra. Bioactivity test showed that Sul-w1 could inhibit tube formation by human microvascular endothelial cells (HMEC) in a dose-dependent manner.
Xu
Yuxia
Y
Glycobiology and Glycochemistry Laboratory, Joint Laboratory for The Research of Chinese Herbal Polysaccharides, Shanghai Institute of Materia Medica, Chinese Academy of Sciences and Infinitus, Shanghai, China.
Dong
Qun
Q
Qiu
Hong
H
Cong
Renhuai
R
Ding
Kan
K
D020533:Angiogenesis Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D002236:Carbohydrate Conformation; D002460:Cell Line; D002850:Chromatography, Gel; D004305:Dose-Response Relationship, Drug; D042783:Endothelial Cells / Q000187:drug effects; D005685:Galactans / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008970:Molecular Weight; D018517:Plant Roots / Q000737:chemistry; D031238:Platycodon / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D013431:Sulfates / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology
D020533:Angiogenesis Inhibitors; D005685:Galactans; D013431:Sulfates; C005653:arabinogalactan