[Expression and clinical significance of SHP2 in the tumor tissues of smokers with lung cancer].
OBJECTIVE
It has been proved that protein phosphorylation and dephosphorylation were important mechanisms in lung cancer development, and tobacco smoking is an important risk factor of lung cancer. The aim of this study is to investigate the expression and clinical significance of protein tyrosine phosphatase SHP2 in non-small cell lung cancer (NSCLC) and small cell lung cancer (SCLC); the relationship between tobacco smoking and the expression of SHP2 is also studied.


METHODS
Immunohistochemistry (Invision) and fluorescence in situ hybridization (FISH) were used to detect the expression of SHP2 and the augment of SHP2 mRNA in the 53 lung cancer specimens.


RESULTS
The weak positive rate of SHP2 was 80% (which was also the total positive rate) in normal bronchial epithelium. The weak, moderate and strong positive rates were 35.4%, 43.8% and 6.2% (total positive rate was 85.4%) in 48 NSCLC patients, 0%, 80% and 20% (total positive rate was 100%) in 5 SCLC patients, 40.7%, 37.4% and 3.7% (total positive rate was 81.5%) in the tumor tissues of 27 NSCLC patients who didn't smoke and 23.8%, 71.4% and 4.7% (total positive rate was 100%) in the tumor tissues of 21 NSCLC patients whose smoking indexes were ≥ 400. Significant differences of SHP2 expression were observed between tumor tissues and normal bronchial epithelium, NSCLC and SCLC, and between different smoking indexes (P<0.05).


CONCLUSIONS
The enhancement of SHP2 expression in the tumor tissues of NSCLC patients who smoke may be correlated with tobacco smoking; SHP2 may play certain role in the development of lung cancer; SHP2 prospectively provides new ideas for the drug research and development of lung cancer treatment.
Zhan
Xuemei
X
Department of Pathology, Linyi People's Hospital of Shandong Province, Linyi 276003, China.
Dong
Hongyan
H
Sun
Chongwei
C
Liu
Lili
L
Wang
Dan
D
Wei
Zhiyong
Z
D000328:Adult; D000368:Aged; D002289:Carcinoma, Non-Small-Cell Lung / Q000201:enzymology* / Q000209:etiology / Q000235:genetics / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000201:enzymology* / Q000209:etiology / Q000235:genetics / Q000473:pathology; D008297:Male; D008875:Middle Aged; D054592:Protein Tyrosine Phosphatase, Non-Receptor Type 11 / Q000235:genetics* / Q000378:metabolism; D055752:Small Cell Lung Carcinoma / Q000201:enzymology* / Q000209:etiology / Q000235:genetics / Q000473:pathology; D012907:Smoking / Q000009:adverse effects / Q000235:genetics* / Q000473:pathology
C516724:PTPN11 protein, human; D054592:Protein Tyrosine Phosphatase, Non-Receptor Type 11