title: Interactions between bacterial isolates from modified-atmosphere-packaged artisan-type cooked ham in view of the development of a bioprotective culture.
abstract: Growth and metabolite production of three dominant bacterial isolates (Carnobacterium divergens 3M14, Leuconostoc carnosum 3M42, and Brochothrix thermosphacta RMS6) from modified-atmosphere-packaged (MAP), artisan-type cooked ham were assessed for their interactions in view of the development of a bioprotective culture. During monoculture experiments in cooked ham simulation medium, Leuc. carnosum 3M42 converted the available glucose into lactic acid and ethanol, whereas the two other strains produced additional metabolites such as acetic acid and 3-methyl-1-butanol. When grown in co-culture, Leuc. carnosum 3M42 suppressed the growth and metabolism of B. thermosphacta RMS6. In contrast, a co-culture of the latter bacterium with C. divergens 3M14 led to a variety of spoilage-related metabolic compounds. Subsequently, experiments with a commercial cooked ham product indicated that Leuc. carnosum 3M42 dominated the meat matrix and improved acceptability of the product over time, hence acting as a bioprotective culture for MAP, artisan-type cooked ham.
authors.affiliation: Research Group of Industrial Microbiology and Food Biotechnology (IMDO), Faculty of Sciences and Bio-engineering Sciences, Vrije Universiteit Brussel, Brussels, Belgium.
authors.forename: Charalampos
authors.initials: C
authors.lastname: Vasilopoulos
authors.forename: Eveline
authors.initials: E
authors.lastname: De Mey
authors.forename: Lore
authors.initials: L
authors.lastname: Dewulf
authors.forename: Hubert
authors.initials: H
authors.lastname: Paelinck
authors.forename: Anny
authors.initials: A
authors.lastname: De Smedt
authors.forename: Frank
authors.initials: F
authors.lastname: Vandendriessche
authors.forename: Luc
authors.initials: L
authors.lastname: De Vuyst
authors.forename: Frédéric
authors.initials: F
authors.lastname: Leroy
mesh_terms: D000818:Animals; D058640:Brochothrix / Q000254:growth & development* / Q000302:isolation & purification / Q000378:metabolism; D056573:Carnobacterium / Q000254:growth & development* / Q000302:isolation & purification / Q000378:metabolism; D002417:Cattle; D005285:Fermentation; D018857:Food Packaging; D006801:Humans; D007934:Leuconostoc / Q000254:growth & development* / Q000302:isolation & purification / Q000378:metabolism; D008461:Meat Products / Q000032:analysis / Q000382:microbiology*; D013649:Taste