[The basic properties of mesenchymal stromal cells from the donor bone marrow: superficial markers].
OBJECTIVE
To characterize a superficial phenotype and to make a cytogenetic analysis of bone marrow (BM) mesenchymal stromal cells (MSC) from donors.


METHODS
The study analyzed BM samples from 11 healthy donors. The phenotype of obtained MSC was analyzed using cytofluorometry. Chromosomal analysis was carried out at the first-second passage.


RESULTS
The superficial phenotype of MSC was steady-state during 8 passages and conformed to the worldwide standard for this cell population. The marker NGFR+ was detectable only during the first 2 passages and the count of CD146+ cells was decreased to 50% as consecutive passages were carried out, which confirms that MSCs have lost their neural and endothelial differentiation capacity. MSCs are stably able to differentiate only into the mesenchymal lineage. The detection of chromosomal rearrangements in MSCs at different stages of cultivation revealed no clonal rearrangements in any case. However, chromosomal aberrations were found 3-10% of metaphases at the first and second passages, which may be associated with chromosome instability in primary cultures.


CONCLUSIONS
The pooled data suggest that the analyzed MSCs meet the conventional worldwide standards.
Svinareva
D A
DA
Shipunova
I N
IN
Ol'shanskaia
Iu V
IuV
Momotiuk
K S
KS
Drize
N I
NI
Savchenko
V G
VG
D015415:Biomarkers / Q000032:analysis; D001854:Bone Marrow Cells / Q000166:cytology* / Q000276:immunology / Q000473:pathology; D051929:CD146 Antigen / Q000032:analysis; D002454:Cell Differentiation / Q000235:genetics / Q000276:immunology; D043171:Chromosomal Instability; D005434:Flow Cytometry; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000276:immunology / Q000473:pathology; D008677:Metaphase / Q000235:genetics / Q000276:immunology; D009419:Nerve Tissue Proteins / Q000032:analysis*; D017475:Receptors, Nerve Growth Factor / Q000032:analysis*; D017154:Stromal Cells / Q000166:cytology / Q000276:immunology / Q000473:pathology; D014019:Tissue Donors
D015415:Biomarkers; D051929:CD146 Antigen; C497902:MCAM protein, human; C484065:NGFR protein, human; D009419:Nerve Tissue Proteins; D017475:Receptors, Nerve Growth Factor