[Construction and identification of a vector inserted with complete genome of poliovirus strain Sabin I].
OBJECTIVE
To develop a vector inserted with complete genome of poliovirus strain Sabin I.


METHODS
The 3 fragments of the complete genome of Sabin I was amplified and cloned to pEASY-T3 by molecular biological technology. These cloned pEASY-T3 were then digested by Restriction enzymes and ligated to pWSK29 step by step and identified.


RESULTS
The complete genome of poliovirus strain Sabin I was successfully cloned into vector pWSK29 with 9 nucleotide mutations.


CONCLUSIONS
The complete genome plasmid was constructed and it provided a basis for further research of the function of Sabin I.
Shen
Hong-Hui
HH
College of Life sciences, Jilin University, Changchun 130012, China.
Hou
Jun
J
Hu
Yan
Y
Bai
Bing-Ke
BK
Wang
Zhi-Jie
ZJ
Yu
Xiang-Hui
XH
Kong
Wei
W
Liu
Ze
Z
Mao
Pan-Yong
PY
D003001:Cloning, Molecular; D005822:Genetic Vectors / Q000235:genetics*; D016679:Genome, Viral; D009154:Mutation; D017955:Poliovirus / Q000235:genetics*