[Human amniotic fluid influenced by different sampling methods during 1H NMR-based metabonomics analysis].
To assess the influence of different sampling methods on Human Amniotic Fluid (HAF) during metabonomics analysis, and to establish a metabolite profile database for normal human amniotic fluid, four experimental groups (the group of freeze-drying, of freeze-thawing, of storage at -20 degrees C, and of keeping in room temperature) and a control group were investigated by use of 1H-NMR spectroscopy, respectively; the data of H-NMR spectroscopy was treated by principal components analysis (PCA). The results showed that, by comparison with the control, there were distinct differences in the experimental groups except the group of storage at -20 degrees C. Therefore, It is possible to use 1H-NMR-based metabonomics technique for analysis of HAF; moreover, during the tests, careful treatments of HAF should be institued to minimize the influence on the samples.
Huang
Han
H
State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610041, China.
Wu
Xiaohua
X
Zhou
Jinglin
J
Xu
Bin
B
Kong
Xiangli
X
Huang
Jing
J
Li
Wei
W
D000653:Amniotic Fluid / Q000378:metabolism*; D005260:Female; D006801:Humans; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D055442:Metabolome; D055432:Metabolomics / Q000379:methods*; D011247:Pregnancy; D025341:Principal Component Analysis; D013048:Specimen Handling / Q000379:methods* / Q000592:standards