Tissue binding patterns and in vitro effects of Campylobacter jejuni DNA-binding protein from starved cells.
Campylobacter jejuni (C. jejuni) is frequently associated with axonal Guillain-Barré syndrome (GBS). We reported that C. jejuni DNA-binding protein from starved cells (C-Dps) binds to and damages myelinated nerves in vivo. We studied the binding patterns of C-Dps to nervous tissues and its in vitro effects on neural cells. Immunohistochemically, C-Dps labeled the nodes of Ranvier, the outermost parts of internodal myelin and the basement membrane in the peripheral nerves, and neurons and myelin in the central nervous tissues. Its binding was blocked by sulfatide. C-Dps bound to the cell surfaces of nerve growth factor (NGF)-treated PC12 cells leading to dose-dependent LDH release, which was inhibited by either heat-denaturation of C-Dps or coincubation with an anti-C-Dps mAb. However, its binding to the surfaces of cultured NSC34 cells, S16 cells, or dorsal root ganglion cells, did not induce cytotoxicity. These findings suggest a possible involvement of C-Dps in C. jejuni-related GBS.
Piao
Hua
H
Department of Neurology, Neurological Institute, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
Minohara
Motozumi
M
Kawamura
Nobutoshi
N
Li
Wei
W
Matsushita
Takuya
T
Yamasaki
Ryo
R
Mizunoe
Yoshimitsu
Y
Kira
Jun-Ichi
J
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002169:Campylobacter Infections / Q000150:complications / Q000473:pathology*; D016123:Campylobacter jejuni / Q000378:metabolism*; D002417:Cattle; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D020275:Guillain-Barre Syndrome / Q000209:etiology / Q000382:microbiology; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D009186:Myelin Sheath / Q000378:metabolism; D009413:Nerve Fibers, Myelinated / Q000378:metabolism* / Q000473:pathology*; D016716:PC12 Cells; D010525:Peripheral Nerves / Q000378:metabolism / Q000473:pathology; D051381:Rats
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D007770:L-Lactate Dehydrogenase