title: Structural and biochemical basis for the firm chemo- and regioselectivity of the nitro-forming N-oxygenase AurF.
abstract: Site-directed mutagenesis based on the crystal structure of AurF, a nitro group forming monooxygenase from Streptomyces thioluteus, revealed that AurF variants are capable of selectively transforming guanidyl- and amidinyl-substituted anilines into the corresponding nitro compounds. Our results provide new insights into the biochemical basis of regioselective N-oxygenation.
authors.affiliation: Leibniz Institute for Natural Product Research, HKI, Beutenbergstr. 11a, 07745 Jena, Germany.
authors.forename: Alexander
authors.initials: A
authors.lastname: Fries
authors.forename: Robert
authors.initials: R
authors.lastname: Winkler
authors.forename: Christian
authors.initials: C
authors.lastname: Hertweck
mesh_terms: D010129:4-Aminobenzoic Acid / Q000737:chemistry / Q000378:metabolism; D020134:Catalytic Domain; D006899:Mixed Function Oxygenases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D009154:Mutation; D009584:Nitrogen / Q000378:metabolism; D010084:Oxidation-Reduction; D013237:Stereoisomerism; D013302:Streptomyces / Q000201:enzymology; D013379:Substrate Specificity
chemical_list: D006899:Mixed Function Oxygenases; D009584:Nitrogen; D010129:4-Aminobenzoic Acid