title: Analysis of Amaryllidaceae alkaloids from Zephyranthes robusta by GC-MS and their cholinesterase activity.
abstract: From the bulbs of Zephyranthes robusta Baker (Amaryllidaceae), seven known compounds, belonging to four structural types of Amaryllidaceae alkaloids, were identified and quantified by GC-MS. The alkaloid extract from the bulbs showed promising acetylcholinesterase and butyrylcholinesterase inhibitory activities against HuAChE (IC50 = 35.9 +/- 3.5 microg/mL) and HuBuChE (IC50 = 190.9 +/- 8.2 microg/mL).
authors.affiliation: Department of Pharmaceutical Botany and Ecology, Faculty of Pharmacy, Charles University, Heyrovského 1203, 500 05 Hradec Králové, Czech Republic. cahlikova@faf.cuni.cz
authors.forename: Lucie
authors.initials: L
authors.lastname: Cahlíková
authors.forename: Andrea
authors.initials: A
authors.lastname: Kulhánková
authors.forename: Klára
authors.initials: K
authors.lastname: Urbanová
authors.forename: Irena
authors.initials: I
authors.lastname: Valterová
authors.forename: Katerina
authors.initials: K
authors.lastname: Macáková
authors.forename: Jirí
authors.initials: J
authors.lastname: Kunes
mesh_terms: D047151:Amaryllidaceae Alkaloids / Q000032:analysis* / Q000494:pharmacology; D002091:Butyrylcholinesterase / Q000378:metabolism; D002800:Cholinesterase Inhibitors / Q000494:pharmacology*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D019668:Liliaceae / Q000737:chemistry*
chemical_list: D047151:Amaryllidaceae Alkaloids; D002800:Cholinesterase Inhibitors; D002091:Butyrylcholinesterase