Anti-inflammatory and gastroprotective properties of Hypericum richeri oil extracts.
Oil extracts of flowering tops of Hypericum richeri Vill. prepared in three different ways were evaluated for chemical composition, and anti-inflammatory and gastroprotective activities. An HPLC method was developed for determination of two dominant flavonoids, quercetin and I3,II8-biapigenin. The carrageenan-induced rat paw edema test was used for screening the anti-inflammatory activity, while indomethacin-induced rat gastric mucosa damage test was used for evaluation of gastroprotective activity. The oil extract prepared by maceration with 96% ethanol, followed by extraction with sunflower oil by heating on a water bath, exhibited the highest anti-inflammatory (38.4%) and gastroprotective activities (gastric damage score of 0.9). The same oil extract had the highest content of quercetin (49 microg/mL) and I3,II8-biapigenin (60 microg/mL). These results approve the usage of oil extracts of H. richeri as an anti-inflammatory and gastroprotective agent.
Zdunić
Gordana
G
Institute for Medicinal Plant Research "Dr Josif Pancić", Tadeusa Kosćuska 1, 11000 Belgrade, Serbia. gzdunic@mocbilja.rs
Godevac
Dejan
D
Milenković
Marina
M
Savikin
Katarina
K
Menković
Nebojsa
N
Petrović
Silvana
S
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D005419:Flavonoids / Q000032:analysis; D005753:Gastric Mucosa / Q000187:drug effects*; D020902:Hypericum / Q000737:chemistry*; D007213:Indomethacin / Q000633:toxicity; D008297:Male; D010936:Plant Extracts / Q000494:pharmacology*; D020011:Protective Agents / Q000494:pharmacology; D011794:Quercetin / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar
D000893:Anti-Inflammatory Agents; D005419:Flavonoids; D010936:Plant Extracts; D020011:Protective Agents; D011794:Quercetin; D007213:Indomethacin