title: A retro-inverso TAT-like peptide designed to deliver cysteamine to cells.
abstract: A retro-inverso, TAT-like peptide wherein lysine residues are replaced with cysteine residues bearing a disulfide-linked cysteamine group is found to engage in thiol-disulfide exchange with cysteine. These peptides are transported into cells and localize to lysosomes. Cellular uptake is enhanced in peptides bearing two cysteamine groups over those with one or none, by factors of approximately 1.5 and 12, respectively.
authors.affiliation: Department of Chemistry Texas A&M University, College Station, TX 77843, USA.
authors.forename: Jongdoo
authors.initials: J
authors.lastname: Lim
authors.forename: Jean-Philippe
authors.initials: JP
authors.lastname: Pellois
authors.forename: Eric E
authors.initials: EE
authors.lastname: Simanek
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003543:Cysteamine / Q000008:administration & dosage*; D003554:Cystinosis; D004220:Disulfides / Q000378:metabolism; D016503:Drug Delivery Systems; D015195:Drug Design; D015696:Gene Products, tat / Q000737:chemistry* / Q000378:metabolism*; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism*; D012190:Retroviridae / Q000235:genetics; D013050:Spectrometry, Fluorescence; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013438:Sulfhydryl Compounds / Q000378:metabolism
chemical_list: D004220:Disulfides; D015696:Gene Products, tat; D010455:Peptides; D013438:Sulfhydryl Compounds; D003543:Cysteamine