title: Lipopolysaccharide from Burkholderia thailandensis E264 provides protection in a murine model of melioidosis.
abstract: Burkholderia thailandensis is a less virulent close relative of Burkholderia pseudomallei, a CDC category B biothreat agent. We have previously shown that lipopolysaccharide (LPS) extracted from B. pseudomallei can provide protection against a lethal challenge of B. pseudomallei in a mouse model of melioidosis. Sugar analysis on LPS from B. thailandensis strain E264 confirmed that this polysaccharide has a similar structure to LPS from B. pseudomallei. Mice were immunised with LPS from B. thailandensis or B. pseudomallei and challenged with a lethal dose of B. pseudomallei strain K96243. Similar protection levels were observed when either LPS was used as the immunogen. This data suggests that B. thailandensis LPS has the potential to be used as part of a subunit based vaccine against pathogenic B. pseudomallei.
authors.affiliation: Dstl Porton Down, Salisbury, Wiltshire SP4 0JQ, UK.
authors.forename: Sarah A
authors.initials: SA
authors.lastname: Ngugi
authors.forename: Valeria V
authors.initials: VV
authors.lastname: Ventura
authors.forename: Omar
authors.initials: O
authors.lastname: Qazi
authors.forename: Sarah V
authors.initials: SV
authors.lastname: Harding
authors.forename: G Barrie
authors.initials: GB
authors.lastname: Kitto
authors.forename: D Mark
authors.initials: DM
authors.lastname: Estes
authors.forename: Anne
authors.initials: A
authors.lastname: Dell
authors.forename: Richard W
authors.initials: RW
authors.lastname: Titball
authors.forename: Timothy P
authors.initials: TP
authors.lastname: Atkins
authors.forename: Katherine A
authors.initials: KA
authors.lastname: Brown
authors.forename: Paul G
authors.initials: PG
authors.lastname: Hitchen
authors.forename: Joann L
authors.initials: JL
authors.lastname: Prior
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000276:immunology / Q000302:isolation & purification; D001428:Bacterial Vaccines / Q000276:immunology; D019117:Burkholderia / Q000276:immunology / Q000472:pathogenicity*; D016957:Burkholderia pseudomallei / Q000276:immunology / Q000472:pathogenicity*; D004195:Disease Models, Animal; D005260:Female; D008070:Lipopolysaccharides / Q000276:immunology* / Q000302:isolation & purification; D008554:Melioidosis / Q000276:immunology / Q000517:prevention & control*; D051379:Mice; D008807:Mice, Inbred BALB C
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001428:Bacterial Vaccines; D008070:Lipopolysaccharides