[Direct cytopathic effects of particular hepatitis B virus genotypes in immunosuppressive condition].
Little is known about the direct cytopathic effect of hepatitis B virus (HBV) and its association with particular viral genotype or genetic mutation. In some immunosuppressed chronic HBV patients who had liver transplantation or HIV-coinfection, high viremia and liver fibrosis may occur. These findings suggest that hepatic injuries could arise in the absence of a mature immune system and the difference of genotype and/or specific mutation would affect cytopathic potential of the virus. We investigate HBV genotype-related differences in viral replication, antigens expression and histopathology using in vitro replication model or uPA/SCID mice harboring human hepatocytes, demonstrating that different HBV genotypes and even particular mutation are associated with different virological and histopathological characteristics. Infection with HBV/C2 as well as PC mutant of the HBV/B1 in immunosuppressive conditions can induce direct cytopathic effect in "humanized" part of the murine liver.
Tanaka
Yasuhito
Y
Department of Virology & Liver Unit, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan. ytanaka@med.nagoya-cu.ac.jp
Sugiyama
Masaya
M
Mizokami
Masashi
M
D000818:Animals; D002478:Cells, Cultured; D005838:Genotype; D006515:Hepatitis B virus / Q000235:genetics* / Q000472:pathogenicity* / Q000502:physiology; D022781:Hepatocytes / Q000473:pathology* / Q000821:virology*; D006801:Humans; D007108:Immune Tolerance; D016031:Liver Transplantation / Q000276:immunology*; D051379:Mice; D009154:Mutation; D014779:Virus Replication / Q000235:genetics