Construction of an artificial glutathione peroxidase active site on copolymer vesicles.
To construct an efficient GPx mimic, a novel method for preparing polymer-based vesicles carrying GPx-active sites was developed. A series of block copolymers loaded with recognition and catalytic sites were synthesized based on polystyrene-block-poly[tri(ethylene glycol) methyl ether acrylate]s (PS-PMEO(3) MAs). By altering the molar ratio of the functional copolymers, vesicles with GPx activity were obtained by self-assembly of these functional copolymers through blending. The optimum GPx mimic constructed by the blending process exhibited high catalytic activity and acted as a real catalyst with typical saturation kinetics behavior. The method may be of benefit for designing other enzyme mimics and may cast a light on constructing other biologically related functional nanoparticles.
Yin
Yanzhen
Y
State Key Laboratory of Supramolecular Structure and Materials, Jilin Univerity, Changchun 130012, People's Republic of China.
Huang
Xin
X
Lv
Chunyan
C
Wang
Liang
L
Yu
Shuangjiang
S
Luo
Quan
Q
Xu
Jiayun
J
Liu
Junqiu
J
D020134:Catalytic Domain / Q000235:genetics*; D005979:Glutathione Peroxidase / Q000138:chemical synthesis* / Q000235:genetics*; D007700:Kinetics; D015394:Molecular Structure; D011092:Polyethylene Glycols / Q000138:chemical synthesis / Q000737:chemistry; D011109:Polymethacrylic Acids / Q000138:chemical synthesis / Q000737:chemistry; D011137:Polystyrenes / Q000737:chemistry; D015202:Protein Engineering / Q000379:methods*; D013691:Tellurium; D022161:Transport Vesicles / Q000378:metabolism
D011109:Polymethacrylic Acids; D011137:Polystyrenes; C466018:poly(triethylene glycol monomethacrylate); D011092:Polyethylene Glycols; D005979:Glutathione Peroxidase; D013691:Tellurium