Novel activity of Rhodobacter sphaeroides spheroidene monooxygenase CrtA expressed in Escherichia coli.
The spheroidene monooxygenase CrtA of Rhodobacter sphaeroides introduces a keto group and/or hydroxy group at the ends of nonnative substrates in Escherichia coli, resulting in the production of novel oxocarotenoids. The heme-containing CrtA is not a P450 enzyme but a new type of oxygenase.
Lee
Pyung Cheon
PC
Department of Molecular Science and Technology, Ajou University, Woncheon-dong, Yeongtong-gu, Suwon 443-749, South Korea. pclee@ajou.ac.kr
Holtzapple
Erik
E
Schmidt-Dannert
Claudia
C
D002338:Carotenoids / Q000302:isolation & purification / Q000378:metabolism; D002855:Chromatography, Thin Layer; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D006899:Mixed Function Oxygenases / Q000235:genetics* / Q000378:metabolism; D030781:Organisms, Genetically Modified / Q000235:genetics / Q000378:metabolism; D012242:Rhodobacter sphaeroides / Q000201:enzymology* / Q000235:genetics
C005637:spheroidene; D002338:Carotenoids; D006899:Mixed Function Oxygenases