DNA-nanoparticle micelles as supramolecular fluorogenic substrates enabling catalytic signal amplification and detection by DNAzyme probes.
Catalytic DNA molecules have tremendous potential in propagating detection events via nucleic acid sequence selective signal amplification. However, they suffer from product inhibition limiting their widespread utility. Herein, this limitation is overcome utilizing a novel fluorogenic substrate design consisting of cooperatively assembled DNA-nanoparticle micelles.
Chien
Miao-Ping
MP
Department of Chemistry & Biochemistry, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA.
Thompson
Matthew P
MP
Gianneschi
Nathan C
NC
D001665:Binding Sites; D055162:Biocatalysis; D021881:DNA, Catalytic / Q000737:chemistry* / Q000378:metabolism*; D004277:DNA, Single-Stranded / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D046911:Macromolecular Substances / Q000737:chemistry; D008823:Micelles; D015394:Molecular Structure; D053758:Nanoparticles / Q000737:chemistry*; D021141:Nucleic Acid Amplification Techniques; D010316:Particle Size; D013379:Substrate Specificity
D021881:DNA, Catalytic; D004277:DNA, Single-Stranded; D005456:Fluorescent Dyes; D046911:Macromolecular Substances; D008823:Micelles