title: Enzyme immobilisation on self-organised nanopatterned electrode surfaces.
abstract: A new method is described for immobilisation of enzymes on polymer-coated Pt islands. These islands are deposited on top of a SAM-covered Au(111) electrode by a combination of electroless and electrochemical deposition, which allows for a variation of island size and distance between the islands. Here we describe the immobilisation of pyranose-2-oxidase (P2Ox) and the catalytic response to D-glucose on such a nanopatterned surface, which provides optimum access to the active centres of the enzyme.
authors.affiliation: Physical Chemistry, Saarland University, 66123 Saarbrücken, Germany.
authors.forename: Janine
authors.initials: J
authors.lastname: Gajdzik
authors.forename: Jennifer
authors.initials: J
authors.lastname: Lenz
authors.forename: Harald
authors.initials: H
authors.lastname: Natter
authors.forename: Rolf
authors.initials: R
authors.lastname: Hempelmann
authors.forename: Gert-Wieland
authors.initials: GW
authors.lastname: Kohring
authors.forename: Friedrich
authors.initials: F
authors.lastname: Giffhorn
authors.forename: Mila
authors.initials: M
authors.lastname: Manolova
authors.forename: Dieter M
authors.initials: DM
authors.lastname: Kolb
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D002237:Carbohydrate Dehydrogenases / Q000737:chemistry / Q000378:metabolism*; D004563:Electrochemistry / Q000379:methods; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D049329:Nanostructures / Q000737:chemistry / Q000648:ultrastructure; D010984:Platinum / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*
chemical_list: D004800:Enzymes, Immobilized; D011108:Polymers; D010984:Platinum; D002237:Carbohydrate Dehydrogenases; C022489:pyranose oxidase; D005947:Glucose