Facile synthesis of metal-chelating magnetic nanoparticles by exploiting organophosphorus coupling.
A new method is described for facile synthesis of metal-chelating magnetic nanoparticles by simply mixing iron oxide nanoparticles with a bifunctional organophosphorus compound, N-(phosphonomethyl)iminodiacetic acid (PM-IDA), in aqueous solution. On charging with nickel ions, the PM-IDA functionalized iron oxide nanoparticles exhibited high His-tag protein binding capacity (0.21 and 0.58 mg/mg for His-tagged green fluorescent protein and chloramphenicol acetyltransferase, respectively) and were successfully used to purify these proteins from bacterial cell extracts to high purity in a single step. Although other synthetic schemes for metal-chelating magnetic nanoparticles have been reported, the method described here is markedly simpler and involves only low-cost reagents.
Yang
Kun
K
Department of Molecular Biosciences and Bioengineering, University of Hawaii, Honolulu, 96822, USA.
Su
Wei Wen
WW
D002614:Chelating Agents / Q000737:chemistry*; D015500:Chloramphenicol O-Acetyltransferase / Q000737:chemistry / Q000302:isolation & purification; D002846:Chromatography, Affinity / Q000379:methods; D005290:Ferric Compounds / Q000737:chemistry; D049452:Green Fluorescent Proteins / Q000737:chemistry / Q000302:isolation & purification; D008280:Magnetics; D053768:Metal Nanoparticles / Q000737:chemistry*; D008670:Metals / Q000737:chemistry*; D009943:Organophosphorus Compounds / Q000737:chemistry*; D011485:Protein Binding
D002614:Chelating Agents; D005290:Ferric Compounds; D008670:Metals; D009943:Organophosphorus Compounds; D049452:Green Fluorescent Proteins; C000499:ferric oxide; D015500:Chloramphenicol O-Acetyltransferase