prdm1a Regulates sox10 and islet1 in the development of neural crest and Rohon-Beard sensory neurons.
The PR domain containing 1a, with ZNF domain factor, gene (prdm1a) plays an integral role in the development of a number of different cell types during vertebrate embryogenesis, including neural crest cells, Rohon-Beard (RB) sensory neurons and the cranial neural crest-derived craniofacial skeletal elements. To better understand how Prdm1a regulates the development of various cell types in zebrafish, we performed a microarray analysis comparing wild type and prdm1a mutant embryos and identified a number of genes with altered expression in the absence of prdm1a. Rescue analysis determined that two of these, sox10 and islet1, lie downstream of Prdm1a in the development of neural crest cells and RB neurons, respectively. In addition, we identified a number of other novel downstream targets of Prdm1a that may be important for the development of diverse tissues during zebrafish embryogenesis.
Olesnicky
Eugenia
E
Department of Craniofacial Biology, University of Colorado, Denver School of Dental Medicine, Aurora, Colorado 80045, USA.
Hernandez-Lagunas
Laura
L
Artinger
Kristin Bruk
KB
D000818:Animals; D030801:Animals, Genetically Modified; D019521:Body Patterning / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D004625:Embryo, Nonmammalian; D018507:Gene Expression Regulation, Developmental; D053263:Gene Regulatory Networks / Q000502:physiology; D018398:Homeodomain Proteins / Q000235:genetics* / Q000378:metabolism; D060850:LIM-Homeodomain Proteins; D009432:Neural Crest / Q000196:embryology* / Q000378:metabolism; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D000074462:Positive Regulatory Domain I-Binding Factor 1; D055757:SOXE Transcription Factors / Q000235:genetics* / Q000378:metabolism; D011984:Sensory Receptor Cells / Q000378:metabolism / Q000502:physiology*; D014157:Transcription Factors; D015027:Zebrafish / Q000196:embryology / Q000235:genetics; D029961:Zebrafish Proteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*
D004268:DNA-Binding Proteins; D018398:Homeodomain Proteins; D060850:LIM-Homeodomain Proteins; D009687:Nuclear Proteins; D055757:SOXE Transcription Factors; D014157:Transcription Factors; D029961:Zebrafish Proteins; C063843:insulin gene enhancer binding protein Isl-1; C442229:sox10 protein, zebrafish; D000074462:Positive Regulatory Domain I-Binding Factor 1; C513257:prdm1a protein, zebrafish