Quantitative screening of EGF receptor-binding peptides by using a peptide library with multiple fluorescent amino acids as fluorescent tags.
EGF receptor-binding peptides could be found by a peptide screening method using fifteen fluorescent amino acids as fluorescent tags. Of 225 peptides, we found an 8-mer peptide containing a dipeptide unit, Y-F, which was the strongest binding peptide to the EGF receptor.
Kitamatsu
Mizuki
M
Department of Medical and Bioengineering, Graduate School of Natural Science and Technology, Okayama University, Kita-ku, Okayama, Japan. kitamatu@cc.okayama-u.ac.jp
Yamamoto
Takahiro
T
Futami
Midori
M
Sisido
Masahiko
M
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry*; D066246:ErbB Receptors / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D015394:Molecular Structure; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding
D000596:Amino Acids; D005456:Fluorescent Dyes; D019151:Peptide Library; D010455:Peptides; D066246:ErbB Receptors