Ethanol fermentation from Jerusalem artichoke powder using Saccharomyces cerevisiae KCCM50549 without pretreatment for inulin hydrolysis.
A strain of Saccharomyces cerevisiae, KCCM50549, was found to efficiently ferment the inulin-containing carbohydrates in Jerusalem artichoke without acidic or enzymatic pretreatment prior to fermentation. S. cerevisiae KCCM50549 could utilize almost completely the fructo-oligosaccharides present in Jerusalem artichoke (up to degree of polymerization (DP) of 15), in contrast to the other S. cerevisiae strain such as NCYC625 that fermented the fructo-oligosaccharides with DP of up to around six. Inulin-fermenting S. cerevisiae KCCM50549 produced c.a. 1.6 times more ethanol from Jerusalem artichoke compared with S. cerevisiae NCYC625. Direct ethanol fermentation of Jerusalem artichoke flour at 180 g/L without any supplements or pretreatments by S. cerevisiae KCCM50549 in a 5 L jar fermentor yielded 36.2 g/L of ethanol within 36 h. The conversion efficiency of inulin-type sugars to ethanol was 70% of the theoretical ethanol yield.
Lim
Seok-Hwan
SH
Industrial Biotechnology Research Center, Daejeon, Republic of Korea.
Ryu
Ji-Myoung
JM
Lee
Hongweon
H
Jeon
Jae Heung
JH
Sok
Dai-Eun
DE
Choi
Eui-Sung
ES
D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D005433:Flour; D006368:Helianthus / Q000378:metabolism*; D006868:Hydrolysis; D007444:Inulin / Q000378:metabolism*; D009844:Oligosaccharides / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism*
D009844:Oligosaccharides; C116580:fructooligosaccharide; D000431:Ethanol; D007444:Inulin