title: Molecular cloning and expression analysis of feline α1-microglobulin.
abstract: Full-length cDNA that encodes feline α₁-microglobulin (Feα₁m)-bikunin was obtained from a feline liver and cloned using an oligo-capping method. The Feα₁m-bikunin cDNA was found to contain 1284 nucleotides, and Feα₁m was found to include an open reading frame encoding a polypeptide of 201 amino acids. The deduced amino acid sequence of Feα₁m showed varying amino acid identity when compared with the published sequences of the related α₁-m of other species, ranging from 71.1 to 82.1%. Feα₁m mRNA expression was confirmed by reverse transcription polymerase chain reaction (RT-PCR) and real-time PCR analysis in the cerebrum, cerebellum, lung, heart, liver, spleen, pancreas, kidney, adrenal gland, and testicle. The highest Feα₁m mRNA level was found in the liver.
authors.affiliation: Department of Small Animal Internal Medicine, School of Veterinary Medicine and Animal Science, Kitasato University, Higashi, Japan.
authors.forename: Junya
authors.initials: J
authors.lastname: Nakata
authors.forename: Aiko
authors.initials: A
authors.lastname: Nakahari
authors.forename: Yohei
authors.initials: Y
authors.lastname: Kato
authors.forename: Nanako
authors.initials: N
authors.lastname: Yamachi
authors.forename: Nami
authors.initials: N
authors.lastname: Sato
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Yonezawa
authors.forename: Chika
authors.initials: C
authors.lastname: Takahashi
authors.forename: Seishiro
authors.initials: S
authors.lastname: Chikazawa
authors.forename: Yasutomo
authors.initials: Y
authors.lastname: Hori
authors.forename: Kazutaka
authors.initials: K
authors.lastname: Kanai
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Ito
authors.forename: Seiichi
authors.initials: S
authors.lastname: Higuchi
authors.forename: Fumio
authors.initials: F
authors.lastname: Hoshi
mesh_terms: D000510:Alpha-Globulins / Q000096:biosynthesis / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002415:Cats / Q000235:genetics; D003001:Cloning, Molecular; D008099:Liver / Q000378:metabolism; D016366:Open Reading Frames / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment
chemical_list: D000510:Alpha-Globulins; D012333:RNA, Messenger; C016001:alpha-1-microglobulin