title: Far-field autofluorescence nanoscopy.
abstract: We demonstrate far-field optical imaging at the nanoscale with unlabeled samples. Subdiffraction resolution images of autofluorescent samples are obtained by depleting the ground state of natural fluorophores by transferring them to a metastable dark state and simultaneously localizing those fluorophores that are transiently returning. Our approach is based on the insight that nanoscopy methods relying on stochastic single-molecule switching require only a single fluorescence on-off cycle to yield an image, a condition fulfilled by various biomolecules. The method is exemplified by recording label-free nanoscopy images of thylakoid membranes of spinach chloroplasts.
authors.affiliation: Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Am Fassberg 11, 37077 Gttingen, Germany.
authors.forename: Jakob
authors.initials: J
authors.lastname: Bierwagen
authors.forename: Ilaria
authors.initials: I
authors.lastname: Testa
authors.forename: Jonas
authors.initials: J
authors.lastname: Fölling
authors.forename: Dirk
authors.initials: D
authors.lastname: Wenzel
authors.forename: Stefan
authors.initials: S
authors.lastname: Jakobs
authors.forename: Christian
authors.initials: C
authors.lastname: Eggeling
authors.forename: Stefan W
authors.initials: SW
authors.lastname: Hell
mesh_terms: D002734:Chlorophyll / Q000032:analysis*; D005456:Fluorescent Dyes / Q000032:analysis; D008856:Microscopy, Fluorescence / Q000379:methods*; D018724:Spinacia oleracea / Q000648:ultrastructure*; D020524:Thylakoids / Q000648:ultrastructure*
chemical_list: D005456:Fluorescent Dyes; D002734:Chlorophyll