title: The effect of magnetic nanoparticles of Fe(3)O(4) on immune function in normal ICR mice.
abstract: We investigated the effect of magnetic nanoparticles of Fe(3)O(4) (Fe(3)O(4)-MNPs) on the mice immune system. Imprinting control region (ICR) mice were assigned randomly into four groups and treated with normal saline or low, medium, or high doses of Fe(3)O(4)-MNPs, respectively. After intravenous administration of Fe(3)O(4)-MNPs for 72 hours, the peripheral T cells and the induction of primary immune responses in mice were investigated by flow cytometry and determined using enzyme-linked immunosorbent assay, respectively. The results showed that the ratio of spleen to body weight was not different between the experimental groups and control group (P > 0.05). The lymphocyte transformation rates in the suspension of spleen were higher in low-dose group than those in the control group (P < 0.05), while the proliferation of splenocytes was low in the medium and high groups when compared to the control group (P < 0.05). In peripheral blood, both the proportions of subset CD4(+) and CD8(+) T lymphocytes in the low-dose group were higher than those in the control group, whereas there was no difference in the number of CD4(+) T cells between the medium- and low-dose groups. Interestingly, the Fe(3)O(4)-MNPs enhanced the production of interleukin-2 (IL-2), interferon-γ, and IL-10 but did not affect the production of IL-4 in peripheral blood. It is concluded that Fe(3)O(4)-MNPs could influence immune functions of normal ICR mice in a dose-dependent manner.
authors.affiliation: Department of Hematology, Zhongda Hospital, Clinical Medical School, Southeast University, Nanjing, People's Republic of China. cba8888@hotmail.com
authors.forename: Bao-An
authors.initials: BA
authors.lastname: Chen
authors.forename: Nan
authors.initials: N
authors.lastname: Jin
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Jiahua
authors.initials: J
authors.lastname: Ding
authors.forename: Chong
authors.initials: C
authors.lastname: Gao
authors.forename: Jian
authors.initials: J
authors.lastname: Cheng
authors.forename: Guohua
authors.initials: G
authors.lastname: Xia
authors.forename: Feng
authors.initials: F
authors.lastname: Gao
authors.forename: Yin
authors.initials: Y
authors.lastname: Zhou
authors.forename: Yue
authors.initials: Y
authors.lastname: Chen
authors.forename: Guina
authors.initials: G
authors.lastname: Zhou
authors.forename: Xiaomao
authors.initials: X
authors.lastname: Li
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.forename: Men
authors.initials: M
authors.lastname: Tang
authors.forename: Xuemei
authors.initials: X
authors.lastname: Wang
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000187:drug effects / Q000276:immunology; D016207:Cytokines / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D005260:Female; D007109:Immunity / Q000187:drug effects*; D008213:Lymphocyte Activation / Q000187:drug effects; D058185:Magnetite Nanoparticles / Q000008:administration & dosage* / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D046529:Microscopy, Electron, Transmission; D050997:Nanomedicine; D010316:Particle Size; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000276:immunology
chemical_list: D016207:Cytokines; D058185:Magnetite Nanoparticles