Neurogenesis in subclasses of vomeronasal sensory neurons in adult mice.
The vomeronasal sensory epithelium contains two distinct populations of vomeronasal sensory neurons. Apical neurons express G(i) (2) (α) -linked V1R vomeronasal receptors and project to the anterior portion of the accessory olfactory bulb, while basal neurons express G(o) (α) -linked V2R receptors and project to the posterior portion. Sensory neurons expressing V1R and V2R vomeronasal receptors are sensitive to different stimuli. Neurons in the vomeronasal system undergo continuous cell turnover during adulthood. To analyze over time neurogenesis of the different sensory cell populations, adult mice were injected with bromodeoxyuridine (BrdU) and sacrificed at postinjection days 1, 3, 5, 7, and 11. Newborn vomeronasal neurons were revealed by antibodies against BrdU while subclasses of vomeronasal neurons were identified using antibodies against G(o) (α) or G(i) (2) (α) proteins. To ascertain whether G proteins are early expressed during neurogenesis, multiple labeling experiments using PSA-NCAM and doublecortin were performed. Distribution of BrdU-labeled cells was analyzed in angular segments from the margin of the sensory epithelium. No sexual differences were found. Within survival groups, BrdU-G(o) (α) labeled cells were found more marginally when compared with BrdU-G(i) (2) (α) labeled cells. The number of BrdU-positive cells decreased from day 1 to day 3 to remain constant afterwards. The relative proportions of BrdU-G(i) (2) (α) and BrdU-G(o) (α) labeled cells remained similar and constant from postinjection day 1 onwards. This rate was also comparable with BrdU-positive cells starting day 3. These results indicate an early, constant, and similar rate of neurogenesis in the two major subclasses of vomeronasal neurons, which suggests that both cell populations maturate independently.
de la Rosa-Prieto
C
C
Departamento de Ciencias Médicas, Universidad de Castilla-La Mancha, 13071 Ciudad Real, Spain.
Saiz-Sanchez
D
D
Ubeda-Bañon
I
I
Argandoña-Palacios
L
L
Garcia-Muñozguren
S
S
Martinez-Marcos
A
A
D000704:Analysis of Variance; D000818:Animals; D005260:Female; D005455:Fluorescent Antibody Technique; D008297:Male; D051379:Mice; D018613:Microscopy, Confocal; D055495:Neurogenesis / Q000502:physiology*; D011984:Sensory Receptor Cells / Q000166:cytology / Q000502:physiology*; D019147:Vomeronasal Organ / Q000166:cytology / Q000502:physiology*