title: Resistant and multi-resistant Gram-positive severe infections: the GISIG working methodology.
abstract: BACKGROUND
  Five thematic working groups composed of Italian infectious disease experts and a group coordinator, supported by a scientific secretary, identified controversial issues in the field of severe healthcare-associated infections caused by multidrug-resistant organisms. The five group coordinators received training courses on data sources and electronic databases, literature search strategies, the scientific revision methods of quality assessment, and the construction of an evidence matrix.
  The working plan identified the following step: definition of the controversial issues and identification of documents for a systematic literature review. A specific methodology to classify the selected evidence was used that required the evaluation of the quality of review documents and statement documents and evaluation of the quality of original research. An original method to assess review documents and statement documents was proposed. A matrix model to extract and evaluate the evidence from original studies was designed using the CONSORT method to evaluate randomized clinical trials and the Newcastle-Ottawa Quality Assessment Scale for case-control studies, cohorts, and retrospective studies.
  
  
  METHODS
  A modified GRADE working group method was applied for grading quality of evidence and strength of recommendations. The working groups reviewed the available studies and formulated recommendations to be voted on at the national consensus conference held in Rome in June 2009.
authors.affiliation: National Institute for Infectious Diseases L. Spallanzani, Rome, Italy. lauria@inmi.it
authors.forename: Francesco N
authors.initials: FN
authors.lastname: Lauria
authors.forename: Gabriella
authors.initials: G
authors.lastname: De Carli
authors.forename: Emanuele
authors.initials: E
authors.lastname: Nicastri
mesh_terms: D003245:Consensus Statements as Topic; D024881:Drug Resistance, Bacterial; D024901:Drug Resistance, Multiple, Bacterial; D005069:Evaluation Studies as Topic; D006094:Gram-Positive Bacteria / Q000187:drug effects* / Q000472:pathogenicity; D016908:Gram-Positive Bacterial Infections / Q000188:drug therapy* / Q000453:epidemiology / Q000382:microbiology; D006287:Health Planning Guidelines; D006801:Humans; D007558:Italy; D016032:Randomized Controlled Trials as Topic / Q000592:standards; D012720:Severity of Illness Index