[The expression of CD95 and activated antigens in peripheral blood lymphocyte subsets in patients with hand foot and mouth disease (HFMD)].
OBJECTIVE
To investigate the expression of CD95 and special marker for activation of peripheral blood lymphocytes in patients with hand foot and mouth disease (HFMD) and its significance.


METHODS
Immunofluorescent two-color flow cytometry was used to study the expression of CD95 and HLA-DR on lymphocytes in 58 patients with HFMD and 34 normal controls.


RESULTS
Expression of CD3+ T cells was significantly lower in patients (63.82 +/- 7.74)% than that in controls (P < 0.001), meanwhile the expression of CD4+ T cells was (34.29 +/- 7.33)%, significantly lower than that of the controls (P < 0.005). The percentage of lymphocytes expressing HLA-DR in patients was (23.77 +/- 5.78)%, significantly higher than that of the controls (P < 0.005). Significant difference was observed in the expression of HLA- DR on CD8+ T cells in patients (1.34 +/- 1.12)% as compared with controls (P < 0.005). No significant difference in the expression of CD95 on lymphocytes was observed between patients and the controls (P > 0.05).


CONCLUSIONS
The findings support that cellular immunodeficiency exists in patients and that lymphocytes were abnormally activated in the patients. The activation of peripheral blood T lymphocytes in patients mainly involves CD8 subset and it may play an important role in the immune response to antiviral infection.
Zhu
Ming-Li
ML
Open laboratory of Hangzhou sixth people's Hospital, Hangzhou 310014, China. mlzhhz@163.com
Zhang
Song-Ping
SP
Cao
He-Qian
HQ
Liang
Wei-Feng
WF
Yu
Jian-Hua
JH
Lou
Guo-Qiang
GQ
D000941:Antigens / Q000235:genetics* / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D016022:Case-Control Studies; D002478:Cells, Cultured; D002675:Child, Preschool; D006232:Hand, Foot and Mouth Disease / Q000235:genetics* / Q000276:immunology*; D006801:Humans; D007223:Infant; D018655:Lymphocyte Count; D008297:Male; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D019014:fas Receptor / Q000235:genetics* / Q000276:immunology
D000941:Antigens; D019014:fas Receptor