title: Bacterial contamination of autogenous bone collected by rongeur compared with that collected by bone filter during implant surgery.
abstract: The aim was to compare the degree of microbial contamination of autogenous bone collected by a bone filter with that of autogenous bone harvested by a rongeur during implant surgery. Thirty healthy patients had dental implants inserted. A strict aspiration protocol was used during the operation to collect particulate bone with minimal risk of contamination by oral flora. A fragment of bone (mainly from the tuberosity) was also harvested with a rongeur. Samples from both groups were sent to the laboratory for the microbes to be counted. All samples yielded viable micro-organisms. There was no significant difference between the number of aerobes in the bone filter and those in the bone fragment group (p=0.9). However, there were significantly more anaerobes in the bone filter group than in the bone fragment group. There were significantly more micro-organisms (both aerobes and anaerobes) in the bone filter group than the bone fragment group (p=0.0001). Even with the use of a stringent aspiration protocol the degree of bacterial contamination was significantly higher in collected bone debris than in bone harvested by rongeur during implant surgery.
authors.affiliation: Shariati Hospital, Tehran University of Medical Sciences, Kargar St., Tehran, Iran. hamid5212@yahoo.com
authors.forename: Hamid Mahmood
authors.initials: HM
authors.lastname: Hashemi
authors.forename: Majid
authors.initials: M
authors.lastname: Beshkar
mesh_terms: D000540:Alveolar Ridge Augmentation / Q000379:methods; D001419:Bacteria / Q000302:isolation & purification*; D001420:Bacteria, Aerobic / Q000302:isolation & purification; D001421:Bacteria, Anaerobic / Q000302:isolation & purification; D058491:Bacterial Load; D016025:Bone Transplantation / Q000295:instrumentation*; D001842:Bone and Bones / Q000382:microbiology*; D003758:Dental Implantation, Endosseous / Q000382:microbiology*; D005374:Filtration / Q000295:instrumentation; D006801:Humans; D010027:Osteotomy / Q000295:instrumentation; D013396:Suction / Q000295:instrumentation; D020858:Tissue and Organ Harvesting / Q000295:instrumentation*