title: Biochemical characterization and sperm motility parameters of ostrich (Struthio camelus) semen.
abstract: The aim of the study was to obtain baseline values for biochemical parameters of ostrich seminal plasma and sperm motility parameters measured by CASA. Biochemical characteristics of ostrich semen included a high protein concentration (29.3 ± 9.1g/l) and high amidase (280.6 ± 130.8 U/l) and LDH activity (1880.0 ± 983.6 U/l). On the other hand antioxidant, superoxide dismutase, anti-proteinase and acid phosphatase activity were low. Biochemical parameters of semen were variable. Motility of ostrich sperm was characterized by low linearity (23.0 ± 6.2%). The quality of undiluted semen stored at room temperature deteriorated within an hour due to agglutination and gelation. On the other hand, ostrich semen could be stored up to 4h at 5°C without loss of motility after which loss of motility occurred but could be partially mitigated using semen extenders (EK and Ovodyl).
authors.affiliation: Department of Semen Biology and Gametes, Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, 10-747 Olsztyn, Poland. a.ciereszko@pan.olsztyn.pl
authors.forename: A
authors.initials: A
authors.lastname: Ciereszko
authors.forename: P K
authors.initials: PK
authors.lastname: Rybnik
authors.forename: J O
authors.initials: JO
authors.lastname: Horbańczuk
authors.forename: G J
authors.initials: GJ
authors.lastname: Dietrich
authors.forename: A
authors.initials: A
authors.lastname: Deas
authors.forename: M
authors.initials: M
authors.lastname: Słowińska
authors.forename: E
authors.initials: E
authors.lastname: Liszewska
authors.forename: I A
authors.initials: IA
authors.lastname: Malecki
mesh_terms: D000135:Acid Phosphatase / Q000032:analysis; D000581:Amidohydrolases / Q000032:analysis*; D000818:Animals; D000975:Antioxidants / Q000032:analysis; D003080:Cold Temperature; D007770:L-Lactate Dehydrogenase / Q000032:analysis*; D008297:Male; D011480:Protease Inhibitors / Q000032:analysis; D011506:Proteins / Q000032:analysis*; D012661:Semen / Q000737:chemistry*; D055101:Semen Analysis / Q000662:veterinary; D012662:Semen Preservation / Q000662:veterinary; D013081:Sperm Motility; D020310:Struthioniformes; D013482:Superoxide Dismutase / Q000032:analysis; D013997:Time Factors
chemical_list: D000975:Antioxidants; D011480:Protease Inhibitors; D011506:Proteins; D007770:L-Lactate Dehydrogenase; D013482:Superoxide Dismutase; D000135:Acid Phosphatase; D000581:Amidohydrolases; C019665:amidase