title: Characterization of a bacterial strain capable of degrading DDT congeners and its use in bioremediation of contaminated soil.
abstract: A bacterial strain DDT-6 (D6) capable of utilizing dichlorodiphenyltrichloroethane (DDT), dichlorodiphenyldichloroethane (DDD), and dichlorodiphenyldichloroethylene (DDE) (DDTs) as its sole carbon and energy source was isolated and identified as Sphingobacterium sp. The degradation of DDTs by strain D6 in mineral salt medium and in field soil was investigated. The half-lives of the degradation of DDTs increased with increasing concentration ranging from 1 to 50 mg L(-1). Favorable degradation conditions for DDTs by strain D6 were found to be pH 7.0 and 30°C. The degradation of DDTs by strain D6 was found to be statistically significantly enhanced (p ≤ 0.05) by the addition of glucose. Based on the metabolites detected, a pathway was proposed for DDT degradation in which it undergoes dechlorination, hydrogenation, dioxygenation, decarboxylation, hydroxylation, and phenyl ring-cleavage reactions to complete the mineralization process. The addition of strain D6 into the contaminated soils was found to statistically significantly enhance (p ≤ 0.05) the degradation of DDTs. The results indicate that the isolate D6 can be used successfully for the removal or detoxification of residues of DDTs in contaminated soil.
authors.affiliation: Department of Plant Protection, College of Agriculture & Biotechnology, Zhejiang University, Hangzhou 310029, Zhejiang, PR China.
authors.forename: Hua
authors.initials: H
authors.lastname: Fang
authors.affiliation: Department of Plant Protection, College of Agriculture & Biotechnology, Zhejiang University, Hangzhou 310029, Zhejiang, PR China.
authors.forename: Bin
authors.initials: B
authors.lastname: Dong
authors.affiliation: Department of Plant Protection, College of Agriculture & Biotechnology, Zhejiang University, Hangzhou 310029, Zhejiang, PR China.
authors.forename: Hu
authors.initials: H
authors.lastname: Yan
authors.affiliation: Department of Plant Protection, College of Agriculture & Biotechnology, Zhejiang University, Hangzhou 310029, Zhejiang, PR China.
authors.forename: Feifan
authors.initials: F
authors.lastname: Tang
authors.affiliation: Department of Plant Protection, College of Agriculture & Biotechnology, Zhejiang University, Hangzhou 310029, Zhejiang, PR China. Electronic address: ylyu@zju.edu.cn.
authors.forename: Yunlong
authors.initials: Y
authors.lastname: Yu
mesh_terms: D001673:Biodegradation, Environmental; D003634:DDT / Q000378:metabolism*; D052918:Environmental Restoration and Remediation / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D007306:Insecticides / Q000378:metabolism*; D013058:Mass Spectrometry; D012989:Soil Pollutants / Q000378:metabolism*; D013696:Temperature
chemical_list: D007306:Insecticides; D012989:Soil Pollutants; D003634:DDT