title: Peptide arrays with a chip.
abstract: Today, lithographic methods enable combinatorial synthesis of >50,000 oligonucleotides per cm(2), an advance that has revolutionized the whole field of genomics. A similar development is expected for the field of proteomics, provided that affordable, very high-density peptide arrays are available. However, peptide arrays lag behind oligonucleotide arrays. This is mainly due to the monomer-by-monomer repeated consecutive coupling of 20 different amino acids associated with lithography, which adds up to an excessive number of coupling cycles. A combinatorial synthesis based on electrically charged solid amino acid particles resolves this problem. A computer chip consecutively addresses the different charged particles to a solid support, where, when completed, the whole layer of solid amino acid particles is melted at once. This frees hitherto immobilized amino acids to couple all 20 different amino acids in one single coupling reaction to the support. The method should allow for the translation of entire genomes into a set of overlapping peptides to be used in proteome research.
authors.affiliation: Institute for Microstructure Technology, Karlsruhe Institute of Technology, Eggenstein-Leopoldshafen, Germany.
authors.forename: Alexander
authors.initials: A
authors.lastname: Nesterov
authors.forename: Edgar
authors.initials: E
authors.lastname: Dörsam
authors.forename: Yun-Chien
authors.initials: YC
authors.lastname: Cheng
authors.forename: Christopher
authors.initials: C
authors.lastname: Schirwitz
authors.forename: Frieder
authors.initials: F
authors.lastname: Märkle
authors.forename: Felix
authors.initials: F
authors.lastname: Löffler
authors.forename: Kai
authors.initials: K
authors.lastname: König
authors.forename: Volker
authors.initials: V
authors.lastname: Stadler
authors.forename: Ralf
authors.initials: R
authors.lastname: Bischoff
authors.forename: Frank
authors.initials: F
authors.lastname: Breitling
mesh_terms: D000596:Amino Acids / Q000737:chemistry; D000818:Animals; D002417:Cattle; D020650:Combinatorial Chemistry Techniques; D004566:Electrodes; D010316:Particle Size; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism*; D011092:Polyethylene Glycols / Q000737:chemistry; D040081:Protein Array Analysis / Q000379:methods*; D013194:Staining and Labeling; D013499:Surface Properties
chemical_list: D000596:Amino Acids; D010455:Peptides; D011092:Polyethylene Glycols