In vitro anti-rotavirus activity of polyphenol compounds isolated from the roots of Glycyrrhiza uralensis.
We evaluated the ability of six polyphenols isolated from the roots of Glycyrrhiza uralensis to inactivate rotaviruses, specially G5P[7] and G8P[7]. Upon finding that all polyphenols possessed anti-rotavirus activity, we evaluated whether these properties were attributable to direct inhibition of the binding of rotavirus to cells and/or to inhibition of viral replication. Using the virucidal assay, we found that all six compounds directly inhibited rotavirus binding, with activity being dependent on the type of virus. The 50% effective inhibitory concentrations (EC(50)) of the six compounds were 18.7-69.5 μM against G5P[7] and 14.7-88.1 μM against G8P[7], respectively. Five of the six compounds inhibited hemagglutination activity. Moreover, the CPE inhibition assay showed that five compounds inhibited viral replication with EC(50) values of 12.1-24.0 μM against G5P[7] and 12.0-42.0 μM against G8P[7], respectively. RT-PCR showed that the compounds suppressed viral RNA synthesis in TF-104 cells. Interestingly, the anti-rotavirus activities of four compounds were attributable to inhibition of both viral absorption and viral replication. These results suggest that compounds isolated from the roots of G. uralensis may be potent anti-rotavirus agents in vivo, acting by inhibiting both viral absorption and viral replication.
Kwon
Hyung-Jun
HJ
Eco-Friendly Biomaterial Research Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup 580-185, Republic of Korea.
Kim
Ha-Hyun
HH
Ryu
Young Bae
YB
Kim
Jang Hoon
JH
Jeong
Hyung Jae
HJ
Lee
Seung-Woong
SW
Chang
Jong Sun
JS
Cho
Kyoung-Oh
KO
Rho
Mun-Chual
MC
Park
Su-Jin
SJ
Lee
Woo Song
WS
D000818:Animals; D000998:Antiviral Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D002417:Cattle; D002460:Cell Line; D005419:Flavonoids / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D029882:Glycyrrhiza uralensis / Q000737:chemistry*; D006384:Hemagglutination / Q000187:drug effects; D008253:Macaca mulatta; D010636:Phenols / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry; D059808:Polyphenols; D012367:RNA, Viral / Q000378:metabolism; D012401:Rotavirus / Q000187:drug effects*; D014779:Virus Replication / Q000187:drug effects
D000998:Antiviral Agents; D005419:Flavonoids; D010636:Phenols; D059808:Polyphenols; D012367:RNA, Viral