Studies on cytotoxic constituents from the skin of the toad Bufo bufo gargarizans.
To study the chemical composition of the skin of Bufo bufo gargarizans, many kinds of chromatography methods were used in the isolation procedures, while the structures of isolated compounds were determined on the basis of their NMR and MS spectral analysis. As a result, two new compounds were isolated from its ethanolic extract and characterized as cinobufotalin 3-nonanedioylarginine ester (8) and bufotalin 3-pimeloylarginine ester (14). Furthermore, 13 known compounds were obtained. Isolated bufadienolides showed significant inhibition effect against SMMC-7721 cell lines in vitro.
Zhao
Hu-Yi
HY
Medical College, Xiamen University, Xiamen, China.
Wu
Fu-Kai
FK
Qiu
Ying-Kun
YK
Wu
Zhen
Z
Jiang
Yong-Tao
YT
Chen
Ji-Yong
JY
D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D002018:Bufanolides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D002025:Bufonidae; D002681:China; D004354:Drug Screening Assays, Antitumor; D015394:Molecular Structure; D012867:Skin / Q000737:chemistry
D000970:Antineoplastic Agents; D002018:Bufanolides; C552791:bufotalin 3-pimeloylarginine ester; C552790:cinobufotalin 3-nonanedioylarginine ester; C004600:bufotalin