title: A genome scan for quantitative trait loci affecting milk somatic cell score in Israeli and Italian Holstein cows by means of selective DNA pooling with single- and multiple-marker mapping.
abstract: Mastitis is an important and common dairy cattle disease affecting milk yield, quality, and consumer safety as well as cheese yields and quality. Animal welfare and residues of the antibiotics used to treat mastitis cause public concern. Considerable genetic variation may allow selection for increased resistance to mastitis. Because of high genetic correlation to milk somatic cell score (SCS), SCS can serve as a surrogate trait for mastitis resistance. The present study intended to identify quantitative trait loci (QTL) affecting SCS in Israeli and Italian Holstein dairy cattle (IsH and ItH, respectively), using selective DNA pooling with single and multiple marker mapping. Milk samples of 4,788 daughters of 6 IsH and 7 ItH sires were used to construct sire-family high- and low-tail pools, which were genotyped at 123 (IsH) and 133 (ItH) microsatellite markers. Shadow correction was used to obtain pool allele frequency estimates. Frequency difference between the tails and empirical standard error of D, SE(D), were used to obtain P-values. All markers significant by single marker mapping were also significant by multiple marker mapping, but not vice versa. Combining both populations, 22 QTL on 21 chromosomes were identified; all corresponded to previous reports in the literature. Confidence intervals were set by chi-squared drop method. Heterozygosity of QTL was estimated at 44.2%. Allele substitution effects ranged from 1,782 to 4,930 cells/mL in estimated breeding value somatic cell count units. Most (80%) of the observed variation in estimated breeding value somatic cell score could be explained by the QTL identified under the stringent criteria. The results found here can be used as a basis for further genome-wide association studies for the same trait.
authors.affiliation: Department of Genetics, The Hebrew University of Jerusalem, 91904 Jerusalem, Israel.
authors.forename: R
authors.initials: R
authors.lastname: Tal-Stein
authors.forename: L
authors.initials: L
authors.lastname: Fontanesi
authors.forename: M
authors.initials: M
authors.lastname: Dolezal
authors.forename: E
authors.initials: E
authors.lastname: Scotti
authors.forename: A
authors.initials: A
authors.lastname: Bagnato
authors.forename: V
authors.initials: V
authors.lastname: Russo
authors.forename: F
authors.initials: F
authors.lastname: Canavesi
authors.forename: A
authors.initials: A
authors.lastname: Friedmann
authors.forename: M
authors.initials: M
authors.lastname: Soller
authors.forename: E
authors.initials: E
authors.lastname: Lipkin
mesh_terms: D000818:Animals; D002417:Cattle / Q000235:genetics*; D002452:Cell Count / Q000662:veterinary*; D002874:Chromosome Mapping / Q000662:veterinary; D004247:DNA / Q000032:analysis*; D005260:Female; D005819:Genetic Markers; D007557:Israel; D007558:Italy; D008297:Male; D008892:Milk / Q000166:cytology*; D040641:Quantitative Trait Loci
chemical_list: D005819:Genetic Markers; D004247:DNA