Subcutaneous tissue reaction to castor oil bean and calcium hydroxide in rats.
Castor oil bean cement (COB) is a new material that has been used as an endodontic sealer, and is a candidate material for direct pulp capping.


OBJECTIVE
The purpose of this study was to evaluate the biocompatibility of a new formulation of COB compared to calcium hydroxide cement (CH) and a control group without any material, in the subcutaneous tissue of rats.


METHODS
The materials were prepared, packed into polyethylene tubes, and implanted in the rat dorsal subcutaneous tissue. Animals were sacrificed at the 7th and 50th days after implantation. A quantitative analysis of inflammatory cells was performed and data were subjected to ANOVA and Tukey's tests at 5% significance level.


RESULTS
Comparing the mean number of inflammatory cells between the two experimental groups (COB and CH) and the control group, statistically significant difference (p=0.0001) was observed at 7 and 50 days. There were no significant differences (p=0.111) between tissue reaction to CH (382 inflammatory cells) and COB (330 inflammatory cells) after 7 days. After 50 days, significantly more inflammatory cells (p=0.02) were observed in the CH group (404 inflammatory cells) than in the COB group (177 inflammatory cells).


CONCLUSIONS
These results demonstrate that the COB cement induces less inflammatory response within long periods.
Camargo
Samira Esteves Afonso
SE
Department of Bioscience and Oral Diagnosis, São José dos Campos Dental School, São Paulo State University, São José dos Campos, SP, Brazil.
Rode
Sigmar de Mello
Sde M
do Prado
Renata Falchete
RF
Carvalho
Yasmin Rodarte
YR
Camargo
Carlos Henrique Ribeiro
CH
D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D002126:Calcium Hydroxide / Q000494:pharmacology*; D002368:Castor Oil / Q000494:pharmacology*; D002452:Cell Count; D003238:Connective Tissue / Q000473:pathology; D004804:Eosinophils / Q000473:pathology; D015726:Giant Cells / Q000473:pathology; D007249:Inflammation / Q000473:pathology; D008214:Lymphocytes / Q000473:pathology; D008264:Macrophages / Q000473:pathology; D008297:Male; D008903:Minerals / Q000494:pharmacology; D018919:Neovascularization, Physiologic / Q000187:drug effects; D009504:Neutrophils / Q000473:pathology; D010950:Plasma Cells / Q000473:pathology; D057872:Pulp Capping and Pulpectomy Agents / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012116:Resins, Plant / Q000494:pharmacology; D016037:Single-Blind Method; D040521:Subcutaneous Tissue / Q000187:drug effects* / Q000473:pathology; D013997:Time Factors
D001672:Biocompatible Materials; D008903:Minerals; D057872:Pulp Capping and Pulpectomy Agents; D012116:Resins, Plant; C509865:poliquil; C025702:Dycal; D002368:Castor Oil; D002126:Calcium Hydroxide