title: Assessment of using laponite cross-linked poly(ethylene oxide) for controlled cell adhesion and mineralization.
abstract: The in vitro cytocompatibility of silicate (Laponite clay) cross-linked poly(ethylene oxide) (PEO) nanocomposite films using MC3T3-E1 mouse preosteoblast cells was investigated while cell adhesion, spreading, proliferation and mineralization were assessed as a function of film composition. By combining the advantageous characteristics of PEO polymer (hydrophilic, prevents protein and cell adhesion) with those of a synthetic and layered silicate (charged, degradable and potentially bioactive) some of the physical and chemical properties of the resulting polymer nanocomposites could be controlled. Hydration, dissolution and mechanical properties were examined and related to cell adhesion. Overall, this feasibility study demonstrates the ability of using model Laponite cross-linked PEO nanocomposites to create bioactive scaffolds.
authors.affiliation: Purdue University, Weldon School of Biomedical Engineering, West Lafayette, IN, USA.
authors.forename: Akhilesh K
authors.initials: AK
authors.lastname: Gaharwar
authors.forename: Patrick J
authors.initials: PJ
authors.lastname: Schexnailder
authors.forename: Benjamin P
authors.initials: BP
authors.lastname: Kline
authors.forename: Gudrun
authors.initials: G
authors.lastname: Schmidt
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002113:Calcification, Physiologic / Q000187:drug effects*; D002448:Cell Adhesion / Q000187:drug effects; D016923:Cell Death / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003432:Cross-Linking Reagents / Q000494:pharmacology*; D008422:Materials Testing; D055595:Mechanical Phenomena / Q000187:drug effects; D051379:Mice; D053761:Nanocomposites / Q000737:chemistry; D053758:Nanoparticles / Q000633:toxicity; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D011092:Polyethylene Glycols / Q000494:pharmacology*; D017640:Silicates / Q000494:pharmacology*; D013499:Surface Properties / Q000187:drug effects
chemical_list: D003432:Cross-Linking Reagents; D017640:Silicates; D011092:Polyethylene Glycols; C524813:laponite; D000469:Alkaline Phosphatase