OCP3 is an important modulator of NPR1-mediated jasmonic acid-dependent induced defenses in Arabidopsis.
BACKGROUND
Upon appropriate stimulation, plants increase their level of resistance against future pathogen attack. This phenomenon, known as induced resistance, presents an adaptive advantage due to its reduced fitness costs and its systemic and broad-spectrum nature. In Arabidopsis, different types of induced resistance have been defined based on the signaling pathways involved, particularly those dependent on salicylic acid (SA) and/or jasmonic acid (JA).


RESULTS
Here, we have assessed the implication of the transcriptional regulator OCP3 in SA- and JA-dependent induced defenses. Through a series of double mutant analyses, we conclude that SA-dependent defense signaling does not require OCP3. However, we found that ocp3 plants are impaired in a Pseudomonas fluorescens WCS417r-triggered induced systemic resistance (ISR) against both Pseudomonas syrinagae DC3000 and Hyaloperonospora arabidopsidis, and we show that this impairment is not due to a defect in JA-perception. Likewise, exogenous application of JA failed to induce defenses in ocp3 plants. In addition, we provide evidence showing that the over-expression of an engineered cytosolic isoform of the disease resistance regulator NPR1 restores the impaired JA-induced disease resistance in ocp3 plants.


CONCLUSIONS
Our findings point to a model in which OCP3 may modulate the nucleocytosolic function of NPR1 in the regulation of JA-dependent induced defense responses.
Ramírez
Vicente
V
Instituto de Biología Molecular y Celular de Plantas (IBMCP), Universidad Politécnica de Valencia-Consejo Superior de Investigaciones Científicas (CSIC), Camino de Vera s/n, Valencia, Spain.
Van der Ent
Sjoerd
S
García-Andrade
Javier
J
Coego
Alberto
A
Pieterse
Corné M J
CM
Vera
Pablo
P
D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D003517:Cyclopentanes / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D007113:Immunity, Innate; D009154:Mutation; D009868:Oomycetes / Q000472:pathogenicity; D054883:Oxylipins / Q000378:metabolism*; D010935:Plant Diseases / Q000235:genetics*; D011551:Pseudomonas fluorescens / Q000472:pathogenicity; D044224:Pseudomonas syringae / Q000472:pathogenicity; D018749:RNA, Plant / Q000235:genetics; D020156:Salicylic Acid / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
D029681:Arabidopsis Proteins; D003517:Cyclopentanes; D018398:Homeodomain Proteins; C105496:NPR1 protein, Arabidopsis; C507065:OCP3 protein, Arabidopsis; D054883:Oxylipins; D018749:RNA, Plant; D014157:Transcription Factors; C011006:jasmonic acid; D020156:Salicylic Acid