Particle properties of sugar maple hemicellulose hydrolysate and its influence on growth and metabolic behavior of Pichia stipitis.
In this study the influence of the insoluble solids in nano-filtrated sugar maple hemicellulosic hydrolysate on the metabolic behavior of Pichia stiptis was investigated. The particle properties of hemicellulosic hydrolysate were analyzed. Phosphoric acid and ammonium (PA) were applied to remove the particles. The metabolic behavior and growth property of P. stipitis in particle--removed hydrolysate was measured. Results demonstrated that the average particle size and zeta potential of the untreated hydrolysate were 2266.9±78.2 nm and -6.09±0.49 mV. Xylose consumption and ethanol production rate were significantly decreased when particle content is greater than 1.63 g/L. Because the majority of particles (34 g/L) were removed from hydrolysates by phosphoric acid and ammonium treatment, the fermentability of the hydrolysate was significantly improved. These results indicated particles play an important role in hydrolysate inhibition effect.
Sun
Zhijie
Z
Biorefinery Research Institute, Department of Paper and Bioprocess Engineering, College of Environment Science and Forestry, State University of Newyork, 1 Forestry Road, Syracuse, NY 13210, USA.
Shupe
Alan
A
Liu
Tingjun
T
Hu
Ruofei
R
Amidon
Thomas E
TE
Liu
Shijie
S
D031002:Acer / Q000737:chemistry*; D003470:Culture Media / Q000494:pharmacology; D005285:Fermentation; D005374:Filtration; D006358:Hot Temperature; D006868:Hydrolysis / Q000187:drug effects; D053758:Nanoparticles / Q000737:chemistry; D010316:Particle Size; D052638:Particulate Matter / Q000737:chemistry*; D010756:Phosphoric Acids / Q000494:pharmacology; D010843:Pichia / Q000166:cytology / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism*; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology*; D055672:Static Electricity; D014867:Water
D003470:Culture Media; D052638:Particulate Matter; D010756:Phosphoric Acids; D011134:Polysaccharides; D014867:Water; C007916:hemicellulose; C030242:phosphoric acid