title: Fractionation, partial characterization and bioactivity of water-soluble polysaccharides and polysaccharide-protein complexes from Pleurotus geesteranus.
abstract: Fractionation and purification of mushroom polysaccharides is a critical process for mushroom clinical application. After a hot-water treatment, the crude Pleurotus geesteranus (PG) was further fractionated into four fractions (PG-1, -2, -3, -4) using gradient precipitation with water and ammonia sulphate. By controlling the initial polymer concentration and ratio of solvents, this process produced PG fractions with high chemical uniformity and narrow Mw distribution without free proteins. Structurally, PG-1 and PG-2 are pure homopolysaccharide mainly composed of glucose; and PG-3 and PG-4 are heteropolysaccharide-protein complexes. PG-2, a high M(w) fraction mainly composed of glucose presented significant cytotoxicity at the concentration of 200 and 100 μg/ml to human breast cancer cells. Here, we report a new mushroom polysaccharides extraction and fractionation method, with which we produced four fractions of PG with PG-2 appearing effective anti-tumour activity.
authors.affiliation: Guelph Food Research Centre, Agriculture and Agri-Food Canada, 93 Stone Road West, Guelph, Ontario, Canada N1G5C9.
authors.forename: Mei
authors.initials: M
authors.lastname: Zhang
authors.forename: Lin
authors.initials: L
authors.lastname: Zhu
authors.forename: Steve W
authors.initials: SW
authors.lastname: Cui
authors.forename: Qi
authors.initials: Q
authors.lastname: Wang
authors.forename: Ting
authors.initials: T
authors.lastname: Zhou
authors.forename: Hengsheng
authors.initials: H
authors.lastname: Shen
mesh_terms: D045744:Cell Line, Tumor; D005591:Chemical Fractionation / Q000379:methods*; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D045424:Complex Mixtures / Q000737:chemistry; D004354:Drug Screening Assays, Antitumor; D005656:Fungal Proteins / Q000494:pharmacology*; D006801:Humans; D006868:Hydrolysis / Q000187:drug effects; D008970:Molecular Weight; D008517:Phytotherapy; D020076:Pleurotus / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D012031:Refractometry; D012995:Solubility / Q000187:drug effects; D014867:Water / Q000737:chemistry*
chemical_list: D045424:Complex Mixtures; D005656:Fungal Proteins; D011134:Polysaccharides; D014867:Water