In vitro and in vivo effects of the Mongolian drug Amu-ru 7 on Helicobacter pylori growth and viability.
Amu-ru 7, a Mongolian folk medicine, is used to treat digestive diseases such as gastritis and gastric and duodenal ulcers. We examined the effect of Amu-ru 7 on the growth and viability of Helicobacter pylori in vivo and in vitro. By the agar dilution method, the MIC of Amu-ru 7 for H. pylori strains was shown to be 100-200 μg/mL with a MIC(90) of 200 μg/mL. Two hundred micrograms per milliliter of Amu-ru 7 exhibited potent bactericidal activity against H. pylori in the stationary phase of growth 6 hr after treatment. Amu-ru 7 inhibited the growth of both AMPC-resistant and CAM-resistant strains, and also had a combined effect with AMPC on AMPC-resistant strain 403. The Amu-ru 7 inhibited biofilm formation by H. pylori and induced morphological changes, such as bleb-like formation and shortening of the cell. Although colonization of the stomach of the Mongolian gerbil by H. pylori was not cured by treatment with Amu-ru 7, both the mean number of H. pylori colonized and the colonization rate were decreased in Amu-ru 7 treated gerbils. These results suggest the effectiveness Amu-ru 7 as an adjunct therapy for eradication therapies consisting of a PPI combined with antibiotics.
Bai
Cui Lan
CL
United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-shi, Tokyo 183-8509, Japan.
Osaki
Takako
T
Yonezawa
Hideo
H
Hanawa
Tomoko
T
Zaman
Cynthia
C
Kurata
Satoshi
S
Kamiya
Shigeru
S
Tanaka
Hideyuki
H
D000818:Animals; D018441:Biofilms / Q000187:drug effects; D005849:Gerbillinae; D016480:Helicobacter pylori / Q000187:drug effects* / Q000254:growth & development / Q000648:ultrastructure; D008297:Male; D056785:Medicine, Mongolian Traditional; D051379:Mice; D008826:Microbial Sensitivity Tests