Development of a real-time qPCR method for detection and enumeration of Mycobacterium spp. in surface water.
A real-time quantitative PCR method was developed for the detection and enumeration of Mycobacterium spp. from environmental samples and was compared to two other methods already described. The results showed that our method, targeting 16S rRNA, was more specific than the two previously published real-time quantitative PCR methods targeting another 16S rRNA locus and the hsp65 gene (100% versus 44% and 91%, respectively).
Radomski
Nicolas
N
Université Paris-Est, Laboratoire Eau Environnement Systèmes Urbains UMR MA 102-AgroParisTech, 6-8 avenue Blaise Pascal Cité Descartes, FR 77455 Champs sur Marne, France. nicolas.radomski@leesu.enpc.fr
Lucas
Françoise S
FS
Moilleron
Régis
R
Cambau
Emmanuelle
E
Haenn
Sophie
S
Moulin
Laurent
L
D058491:Bacterial Load / Q000379:methods; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics; D008969:Molecular Sequence Data; D009161:Mycobacterium / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012680:Sensitivity and Specificity; D014871:Water Microbiology
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S