Paeonol inhibits tumor growth in gastric cancer in vitro and in vivo.
OBJECTIVE
To investigate the anti-tumor effects of paeonol in gastric cancer cell proliferation and apoptosis in vitro and in vivo.


METHODS
Murine gastric cancer cell line mouse forestomach carcinoma (MFC) or human gastric cancer cell line SGC-7901 was cultured in the presence or absence of paeonol. Cell proliferation was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, and cell cycle and apoptosis by flow cytometry and TUNEL staining. Tumor growth after subcutaneous implantation of MFC cells in mice was monitored, and the effects of treatment with paeonol were determined.


RESULTS
In vitro, paeonol caused dose-dependent inhibition on cell proliferation and induced apoptosis. Cell cycle analysis revealed a decreased proportion of cells in G0/G1 phase, with arrest at S. Paeonol treatment in gastric cancer cell line MFC and SGC-790 cells significantly reduced the expression of Bcl-2 and increased the expression of Bax in a concentration-related manner. Administration of paeonol to MFC tumor-bearing mice significantly lowered the tumor growth and caused tumor regression.


CONCLUSIONS
Paeonol has significantly growth-inhibitory and apoptosis-inducing effects in gastric cancer cells both in vitro and in vivo.
Li
Na
N
Department of Oncology, the First Affiliated Hospital of Anhui Medical University, Hefei 230022, Anhui Province, China.
Fan
Lu-Lu
LL
Sun
Guo-Ping
GP
Wan
Xin-An
XA
Wang
Zhang-Gui
ZG
Wu
Qiang
Q
Wang
Hua
H
D000098:Acetophenones / Q000494:pharmacology / Q000627:therapeutic use*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008297:Male; D051379:Mice; D013274:Stomach Neoplasms / Q000188:drug therapy / Q000473:pathology; D051028:bcl-2-Associated X Protein / Q000378:metabolism
D000098:Acetophenones; D051028:bcl-2-Associated X Protein; C013638:paeonol