title: Visualizing the functional diversification of CD8+ T cell responses in lymph nodes.
abstract: CD8(+) T cell responses generate effector cells endowed with distinct functional potentials but the contribution of early events in this process is unclear. Here, we have imaged T cells expressing a fluorescent reporter for the activation of the interferon-γ (IFN-γ) locus during priming in lymph nodes. We have demonstrated marked differences in the efficiency of gene activation during stable T cell-dentritic cell (DC) contacts, influenced in part by signal strength. Imaging the first cell division, we have demonstrated that heterogeneity in T cell functional potential was largely apparent as T cells initiated clonal expansion. Moreover, by analyzing the fate of single activated T cells ex vivo, we have provided evidence that these early differences resulted in clonal progenies with distinct functional properties. Thus, the early set of T cell-DC interactions in lymph nodes largely contribute to the heterogeneity of T cell responses through the generation of functionally divergent clonal progenies.
authors.affiliation: Institut Pasteur, Unité des Dynamiques des Réponses Immunes, Département d'Immunologie, Paris, France.
authors.forename: Hélène
authors.initials: H
authors.lastname: Beuneu
authors.forename: Fabrice
authors.initials: F
authors.lastname: Lemaître
authors.forename: Jacques
authors.initials: J
authors.lastname: Deguine
authors.forename: Hélène D
authors.initials: HD
authors.lastname: Moreau
authors.forename: Isabelle
authors.initials: I
authors.lastname: Bouvier
authors.forename: Zacarias
authors.initials: Z
authors.lastname: Garcia
authors.forename: Matthew L
authors.initials: ML
authors.lastname: Albert
authors.forename: Philippe
authors.initials: P
authors.lastname: Bousso
mesh_terms: D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002450:Cell Communication; D003713:Dendritic Cells / Q000276:immunology; D006412:Hematopoietic Stem Cells / Q000502:physiology; D007371:Interferon-gamma / Q000235:genetics; D008198:Lymph Nodes / Q000276:immunology*; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D007371:Interferon-gamma