title: Gene signatures in wound tissue as evidenced by molecular profiling in the chick embryo model.
abstract: BACKGROUND
  Modern functional genomic approaches may help to better understand the molecular events involved in tissue morphogenesis and to identify molecular signatures and pathways. We have recently applied transcriptomic profiling to evidence molecular signatures in the development of the normal chicken chorioallantoic membrane (CAM) and in tumor engrafted on the CAM. We have now extended our studies by performing a transcriptome analysis in the "wound model" of the chicken CAM, which is another relevant model of tissue morphogenesis.
  
  
  RESULTS
  To induce granulation tissue (GT) formation, we performed wounding of the chicken CAM and compared gene expression to normal CAM at the same stage of development. Matched control samples from the same individual were used. We observed a total of 282 genes up-regulated and 44 genes down-regulated assuming a false-discovery rate at 5% and a fold change > 2. Furthermore, bioinformatics analysis lead to the identification of several categories that are associated to organismal injury, tissue morphology, cellular movement, inflammatory disease, development and immune system. Endothelial cell data filtering leads to the identification of several new genes with an endothelial cell signature.
  
  
  CONCLUSIONS
  The chick chorioallantoic wound model allows the identification of gene signatures and pathways involved in GT formation and neoangiogenesis. This may constitute a fertile ground for further studies.
authors.affiliation: INSERM, U920, 3340 Talence, France.
authors.forename: Fabienne
authors.initials: F
authors.lastname: Soulet
authors.forename: Witold W
authors.initials: WW
authors.lastname: Kilarski
authors.forename: Philipp
authors.initials: P
authors.lastname: Antczak
authors.forename: John
authors.initials: J
authors.lastname: Herbert
authors.forename: Roy
authors.initials: R
authors.lastname: Bicknell
authors.forename: Francesco
authors.initials: F
authors.lastname: Falciani
authors.forename: Andreas
authors.initials: A
authors.lastname: Bikfalvi
mesh_terms: D000818:Animals; D002642:Chick Embryo; D049033:Chorioallantoic Membrane / Q000378:metabolism* / Q000473:pathology*; D016000:Cluster Analysis; D019295:Computational Biology; D015536:Down-Regulation / Q000235:genetics; D042783:Endothelial Cells / Q000378:metabolism; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D053263:Gene Regulatory Networks / Q000235:genetics; D006097:Granulation Tissue / Q000378:metabolism / Q000473:pathology; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008954:Models, Biological; D015854:Up-Regulation / Q000235:genetics; D014945:Wound Healing / Q000235:genetics*