title: Autotransplantation of cryopreserved ovarian tissue in 12 women with chemotherapy-induced premature ovarian failure: the Danish experience.
abstract: OBJECTIVE
  To describe a cohort of 12 Danish women who received autotransplantation of frozen-thawed cryopreserved ovarian tissue because of premature ovarian failure after cancer treatment.
  
  
  METHODS
  Retrospective study.
  
  
  METHODS
  University hospitals.
  
  
  METHODS
  Twelve women with autotransplanted frozen-thawed ovarian tissue.
  
  
  METHODS
  Monitoring of hormonal parameters and results of 56 IVF cycles in 10 women.
  
  
  METHODS
  Levels of gonadotropins and sex steroids, functional life span of the grafts, and results of IVF.
  
  
  RESULTS
  All 12 women regained ovarian function between 8 and 26 weeks (mean 19 weeks) after transplantation. Ten women underwent a total of 56 IVF cycles, 76 follicles developed, 49 oocytes were aspirated, 18 were fertilized, and 16 embryos were transferred resulting in six pregnancies: two biochemical, one clinical that miscarried in week 7, and two ongoing resulting in the delivery of two healthy infants born at term to two women. One of these women subsequently conceived spontaneously and delivered another healthy infant. The life span of the transplanted tissue has been between 6 months and still functioning after 54 months.
  
  
  CONCLUSIONS
  Autotransplantation consistently leads to recovery of ovarian function after treatment-induced ovarian failure. Four women became pregnant, after IVF or spontaneously, resulting in the delivery of three healthy infants.
authors.affiliation: The Fertility Clinic, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark. kirsten.tryde.schmidt@rh.regionh.dk
authors.forename: Kirsten Tryde
authors.initials: KT
authors.lastname: Schmidt
authors.forename: Mikkel
authors.initials: M
authors.lastname: Rosendahl
authors.forename: Erik
authors.initials: E
authors.lastname: Ernst
authors.forename: Anne
authors.initials: A
authors.lastname: Loft
authors.forename: Anders Nyboe
authors.initials: AN
authors.lastname: Andersen
authors.forename: Margit
authors.initials: M
authors.lastname: Dueholm
authors.forename: Christian
authors.initials: C
authors.lastname: Ottosen
authors.forename: Claus Yding
authors.initials: CY
authors.lastname: Andersen
mesh_terms: D000328:Adult; D000970:Antineoplastic Agents / Q000009:adverse effects* / Q000627:therapeutic use; D015925:Cryopreservation; D003718:Denmark; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D007247:Infertility, Female / Q000139:chemically induced / Q000534:rehabilitation / Q000628:therapy; D009369:Neoplasms / Q000188:drug therapy / Q000534:rehabilitation; D010053:Ovary / Q000502:physiology / Q000637:transplantation*; D011247:Pregnancy; D016649:Primary Ovarian Insufficiency / Q000139:chemically induced* / Q000534:rehabilitation / Q000628:therapy*; D020127:Recovery of Function; D012189:Retrospective Studies; D014182:Transplantation, Autologous; D055815:Young Adult
chemical_list: D000970:Antineoplastic Agents