Increase of fecundity by probiotic administration in zebrafish (Danio rerio).
It is well known established that reproduction is sensitive to the state of energy reserves, and that there is a balance between energy homeostasis and fertility. In this view, this study examined the effects of the probiotic Lactobacillus rhamnosus, as a feed additive, on zebrafish (Danio rerio) fecundity. Ten days of probiotic treatment modulate the gene expression of neuropeptide hormones and metabolic signals, such as kiss1, kiss2 and leptin both at the CNS level and at the peripheral level. The increase in fecundity brought about by the probiotic was demonstrated by the higher number of ovulated eggs in vivo and by the higher germinal vesicle breakdown rate obtained with the in vitro maturation assay. The increase in oocyte maturation was associated with increased transcription of genes coding for signals which induce the maturation phase, such as lhcgr, cbr1l, and paqr8 genes, concomitant with a decreased transcription of genes coding for local factors which prevent oocyte maturation, such as tgfb1, gdf9, and bmp15. In conclusion, all these findings highlight the action of L. rhamnosus both on the endocrine system and at the local level by inducing oocyte maturation. The significance of the results herein obtained underlined the importance of diet in the reproductive process, supporting the hypothesis that feed additives can improve fecundity. Considering that the zebrafish has been clearly established as a vertebrate model for biomedical research, these results support the potentiality of feed additives such as probiotics, frequently used in the human diet, as a new technology to improve reproduction in all vertebrates, including humans.
Gioacchini
Giorgia
G
Department of Marine Sciences, Polytechnic University of Marche, via Brecce Bianche, 60131 Ancona, Italy.
Maradonna
Francesca
F
Lombardo
Francesco
F
Bizzaro
Davide
D
Olivotto
Ike
I
Carnevali
Oliana
O
D000818:Animals; D004734:Energy Metabolism / Q000187:drug effects / Q000235:genetics; D005260:Female; D005298:Fertility / Q000187:drug effects* / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects; D052201:Lacticaseibacillus rhamnosus / Q000502:physiology; D008297:Male; D009865:Oocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D009866:Oogenesis / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D019936:Probiotics / Q000008:administration & dosage / Q000494:pharmacology*; D012098:Reproduction / Q000187:drug effects / Q000235:genetics; D015027:Zebrafish / Q000235:genetics / Q000378:metabolism / Q000502:physiology*