A thiophen-thiooxorhodamine conjugate fluorescent probe for detecting mercury in aqueous media and living cells.
A rhodamine-based sensor RB-S2 was designed and synthesized by combination of the thiospirolacton chromophore and the thiophen ring block with high affinity to Hg(2+). Probe RB-S2 exhibits high selectivity and excellent sensitivity in both absorbance and fluorescence detection of Hg(2+) in aqueous solution. In addition, fluorescent imaging of Hg(2+) in MCF-7 cells is also successfully demonstrated.
Zhou
Yi
Y
College of Science and State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing University of Technology, Nanjing 210009, P. R. China.
You
Xue-Yan
XY
Fang
Yuan
Y
Li
Ju-Ying
JY
Liu
Ke
K
Yao
Cheng
C
D045744:Cell Line, Tumor; D002470:Cell Survival; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D008628:Mercury / Q000032:analysis*; D008856:Microscopy, Fluorescence / Q000379:methods; D012235:Rhodamines / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods; D013876:Thiophenes / Q000737:chemistry*
D005456:Fluorescent Dyes; D012235:Rhodamines; D013876:Thiophenes; D008628:Mercury