title: Amniotic membrane transplantation for the treatment of feline corneal sequestrum: pilot study.
abstract: OBJECTIVE
  To describe and evaluate the use of equine amniotic membrane trans-plantation after lamellar keratectomy for the treatment of corneal sequestrum in cats.
  
  
  METHODS
  Six cats (seven eyes) of various breed and ages with corneal sequestra were treated surgically with lamellar keratectomy and amniotic membrane transplantation. All the sequestra and a small piece of the amniotic membranes used for each surgery were submitted for histopathologic examination.
  
  
  RESULTS
  Five of the seven eyes showed minimal level of scarring in the cornea and good transparency. No recurrences of the sequestra have been noted during the follow-up period (3-9 months). One eye had necrosis of the amniotic membrane 2 weeks after the surgery. The sequestrum of this eye showed a high level of bacterial contamination on histopathology. Three months later the same cat developed a descemetocele in the area where the necrotic amniotic membrane was rejected. A second eye developed a perforation under the amniotic membrane two weeks after the surgery. The sequestrum of this eye was deep and without vascularization.
  
  
  CONCLUSIONS
  Amniotic membrane transplantation after lamellar keratectomy was a valid procedure for surgical treatment of corneal sequestrum in cats. The procedure resulted in excellent cosmesis and functional vision in five of seven eyes; although case selection is important, particularly to exclude the very deep and non-vascularized sequestra.
authors.affiliation: Dipartimento di Scienze Cliniche Veterinarie, Università degli Studi di Milano, Milano, Italy. laura.barachetti@unimi.it
authors.forename: Laura
authors.initials: L
authors.lastname: Barachetti
authors.forename: Chiara
authors.initials: C
authors.lastname: Giudice
authors.forename: Carlo M
authors.initials: CM
authors.lastname: Mortellaro
mesh_terms: D000818:Animals; D001684:Biological Dressings / Q000662:veterinary*; D002415:Cats; D003316:Corneal Diseases / Q000628:therapy*; D005260:Female; D008297:Male; D013508:Ophthalmologic Surgical Procedures; D010865:Pilot Projects