[Hemocompatibility evaluation in vitro of small-caliber expanded polytetrafluoroethylene vessel with silk fibroin coating sulfonated by low temperature plasma].
OBJECTIVE
To evaluate the hemocompatibility of a small-caliber expanded polytetrafluoroethylene vessel with silk fibroin coating sulfonated by low temperature plasma treatment.


METHODS
The composite blood vessel was prepared by first coating the small-caliber expanded polytetrafluoroethylene vessel with silk fibroin followed by sulfonation by low temperature plasma treatment. After hemolysis test in vitro, dynamic coagulation time test, blood platelet adhesion test, and recalcification time test were performed to evaluate the hemocompatibility of the composite blood vessel.


RESULTS
Scanning electronic microscopy revealed obvious platelets adhesion on the conventional artificial (control) vessel, which seldom occurred on the composite vessel. The curve of absorbance-clotting time of the composite vessel declined more slowly than that of the control vessel. The recalcification time of the composite blood vessel averaged 603 s, significantly longer than that of the control vessel (480 s, P = 0.000).


CONCLUSIONS
The composite blood vessel has good antithrombotic activity and hemocompatibility as a promising vascular prosthesis.
Li
Shao-bin
SB
Department of Cardiothoracic Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China. lsb19821023.student@sina.com
Yan
Yu-sheng
YS
Li
Hui
H
Chen
Kun-tang
KT
Tong
Jian
J
Chen
Qun-qing
QQ
Zhang
Fu-wei
FW
Xiao
Fei
F
D001807:Blood Vessel Prosthesis; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D003080:Cold Temperature; D005349:Fibroins / Q000737:chemistry*; D006801:Humans; D008422:Materials Testing; D058626:Plasma Gases; D011138:Polytetrafluoroethylene / Q000737:chemistry*; D013451:Sulfonic Acids / Q000737:chemistry
D020099:Coated Materials, Biocompatible; D058626:Plasma Gases; D013451:Sulfonic Acids; C482288:fibroin, silkworm; D011138:Polytetrafluoroethylene; D005349:Fibroins