title: High-throughput screening of improved protease inhibitors using a yeast cell surface display system and a yeast cell chip.
abstract: Protease-targeted inhibitors have been promising pharmaceuticals. Here, we combined a yeast cell surface display system with a yeast cell chip for the high-throughput screening of protease inhibitors, and succeeded in improving the activity of a protease inhibitor.
authors.affiliation: Japan Society for the Promotion of Science, Sakyo-ku, Kyoto 606-8502, Japan.
authors.forename: Wataru
authors.initials: W
authors.lastname: Aoki
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Yoshino
authors.forename: Hironobu
authors.initials: H
authors.lastname: Morisaka
authors.forename: Keiji
authors.initials: K
authors.lastname: Tsunetomo
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Koyo
authors.forename: Shinji
authors.initials: S
authors.lastname: Kamiya
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Kawata
authors.forename: Kouichi
authors.initials: K
authors.lastname: Kuroda
authors.forename: Mitsuyoshi
authors.initials: M
authors.lastname: Ueda
mesh_terms: D004353:Drug Evaluation, Preclinical; D057166:High-Throughput Screening Assays / Q000379:methods*; D007147:Immunoglobulin Light Chains / Q000737:chemistry; D008666:Metalloendopeptidases / Q000037:antagonists & inhibitors; D011480:Protease Inhibitors / Q000494:pharmacology*; D011993:Recombinant Fusion Proteins / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000378:metabolism
chemical_list: D007147:Immunoglobulin Light Chains; D011480:Protease Inhibitors; D011993:Recombinant Fusion Proteins; D008666:Metalloendopeptidases; C062316:neurolysin