Co-administration of protein drugs with gold nanoparticles to enable percutaneous delivery.
An interesting nanoscale interfacial phenomenon mediated by gold nanoparticles (Au-NPs) was found, in that co-administration with Au-NPs enables percutaneous delivery of protein drugs. The Au-NPs with a mean size of 5 nm were revealed to be skin permeable, presumably due to the nano-bio interaction with skin lipids and the consequent induction of transient and reversible openings on the stratum corneum. Importantly, when simultaneously applied with Au-NPs, the protein drugs were also granted the ability to penetrate the skin barrier and migrate into the deep layers. This indicated that co-administration with the skin-permeable Au-NPs could mediate proteins across the skin barrier. Such co-delivery effect highlights a simple yet effective method for overcoming the skin barrier for percutaneous protein drug delivery. Employing this method, a non-invasive vaccine delivery strategy was developed, and by topically co-administrating antigens with Au-NPs, robust immune responses were elicited in the tested animals. The results provide the promise for achieving a needleless and self-administrable transcutaneous vaccination.
Huang
Yongzhuo
Y
Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 501 Hai-ke Road, Shanghai 201203, China.
Yu
Faquan
F
Park
Yoon-Shin
YS
Wang
Jianxin
J
Shin
Meong-Cheol
MC
Chung
Hee Sun
HS
Yang
Victor C
VC
D000279:Administration, Cutaneous; D000818:Animals; D016923:Cell Death / Q000187:drug effects; D016503:Drug Delivery Systems; D006046:Gold / Q000008:administration & dosage* / Q000494:pharmacology; D006801:Humans; D007114:Immunization; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects; D053768:Metal Nanoparticles / Q000008:administration & dosage* / Q000648:ultrastructure; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000276:immunology; D010316:Particle Size; D011506:Proteins / Q000008:administration & dosage* / Q000494:pharmacology / Q000627:therapeutic use*; D012869:Skin Absorption / Q000187:drug effects
D011506:Proteins; D006046:Gold; D010047:Ovalbumin