Improved DNA vaccination by skin-targeted delivery using dry-coated densely-packed microprojection arrays.
HSV-2-gD2 DNA vaccine was precisely delivered to immunologically sensitive regions of the skin epithelia using dry-coated microprojection arrays. These arrays delivered a vaccine payload to the epidermis and the upper dermis of mouse skin. Immunomicroscopy results showed that, in 43 ± 5% of microprojection delivery sites, the DNA vaccine was delivered to contact with professional antigen presenting cells in the epidermal layer. Associated with this efficient delivery of the vaccine into the vicinity of the professional antigen presenting cells, we achieved superior antibody responses and statistically equal protection rate against an HSV-2 virus challenge, when compared with the mice immunized with intramuscular injection using needle and syringe, but with less than 1/10th of the delivered antigen.
Chen
Xianfeng
X
The University of Queensland, Delivery of Drugs and Genes Group, Australian Institute for Bioengineering and Nanotechnology, Brisbane, Queensland 4072, Australia.
Kask
Angela Shaulov
AS
Crichton
Michael L
ML
McNeilly
Celia
C
Yukiko
Sally
S
Dong
Lichun
L
Marshak
Joshua O
JO
Jarrahian
Courtney
C
Fernando
Germain J P
GJ
Chen
Dexiang
D
Koelle
David M
DM
Kendall
Mark A F
MA
D000279:Administration, Cutaneous; D000818:Animals; D000917:Antibody Formation; D016503:Drug Delivery Systems / Q000295:instrumentation*; D005260:Female; D006558:Herpes Genitalis / Q000517:prevention & control*; D018258:Herpesvirus 2, Human / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D012867:Skin / Q000378:metabolism*; D012869:Skin Absorption; D014611:Vaccination / Q000295:instrumentation*; D019444:Vaccines, DNA / Q000008:administration & dosage*
D019444:Vaccines, DNA