Defining the structural requirements for ribose 5-phosphate-binding and intersubunit cross-talk of the malarial pyridoxal 5-phosphate synthase.
Most organisms synthesise the B(6) vitamer pyridoxal 5-phosphate (PLP) via the glutamine amidotransferase PLP synthase, a large enzyme complex of 12 Pdx1 synthase subunits with up to 12 Pdx2 glutaminase subunits attached. Deletion analysis revealed that the C-terminus has four distinct functionalities: assembly of the Pdx1 monomers, binding of the pentose substrate (ribose 5-phosphate), formation of the reaction intermediate I(320), and finally PLP synthesis. Deletions of distinct C-terminal regions distinguish between these individual functions. PLP formation is the only function that is conferred to the enzyme by the C-terminus acting in trans, explaining the cooperative nature of the complex.
Derrer
Bianca
B
University Hospital Heidelberg, Department of Infectious Diseases, Parasitology, Heidelberg, Germany.
Windeisen
Volker
V
Guédez Rodríguez
Gabriela
G
Seidler
Joerg
J
Gengenbacher
Martin
M
Lehmann
Wolf D
WD
Rippe
Karsten
K
Sinning
Irmgard
I
Tews
Ivo
I
Kappes
Barbara
B
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016054:DNA, Protozoan / Q000235:genetics; D005972:Glutaminase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D007700:Kinetics; D008958:Models, Molecular; D008969:Molecular Sequence Data; D046912:Multiprotein Complexes; D010963:Plasmodium falciparum / Q000201:enzymology* / Q000235:genetics; D021122:Protein Subunits; D015800:Protozoan Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D011732:Pyridoxal Phosphate / Q000096:biosynthesis*; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012267:Ribosemonophosphates / Q000378:metabolism*; D017384:Sequence Deletion
D016054:DNA, Protozoan; D046912:Multiprotein Complexes; D021122:Protein Subunits; D015800:Protozoan Proteins; D011994:Recombinant Proteins; D012267:Ribosemonophosphates; C031626:ribose-5-phosphate; D011732:Pyridoxal Phosphate; D005972:Glutaminase