title: The crystal structure of a bacterial Sufu-like protein defines a novel group of bacterial proteins that are similar to the N-terminal domain of human Sufu.
abstract: Sufu (Suppressor of Fused), a two-domain protein, plays a critical role in regulating Hedgehog signaling and is conserved from flies to humans. A few bacterial Sufu-like proteins have previously been identified based on sequence similarity to the N-terminal domain of eukaryotic Sufu proteins, but none have been structurally or biochemically characterized and their function in bacteria is unknown. We have determined the crystal structure of a more distantly related Sufu-like homolog, NGO1391 from Neisseria gonorrhoeae, at 1.4 Å resolution, which provides the first biophysical characterization of a bacterial Sufu-like protein. The structure revealed a striking similarity to the N-terminal domain of human Sufu (r.m.s.d. of 2.6 Å over 93% of the NGO1391 protein), despite an extremely low sequence identity of ∼15%. Subsequent sequence analysis revealed that NGO1391 defines a new subset of smaller, Sufu-like proteins that are present in ∼200 bacterial species and has resulted in expansion of the SUFU (PF05076) family in Pfam.
authors.affiliation: Joint Center for Structural Genomics. http://www.jcsg.org
authors.forename: Debanu
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authors.lastname: Das
authors.forename: Robert D
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authors.forename: Polat
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authors.lastname: Abdubek
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authors.lastname: Duan
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authors.lastname: Ellrott
authors.forename: Carol L
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authors.lastname: Farr
authors.forename: Julie
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authors.lastname: Feuerhelm
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authors.forename: Anna
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authors.lastname: Grzechnik
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authors.forename: Lukasz
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authors.lastname: Jaroszewski
authors.forename: Kevin K
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authors.forename: Heath E
authors.initials: HE
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authors.forename: Mark W
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authors.lastname: Knuth
authors.forename: Piotr
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authors.lastname: Kozbial
authors.forename: S Sri
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authors.lastname: Krishna
authors.forename: Abhinav
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authors.forename: Winnie W
authors.initials: WW
authors.lastname: Lam
authors.forename: David
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authors.lastname: Marciano
authors.forename: Mitchell D
authors.initials: MD
authors.lastname: Miller
authors.forename: Andrew T
authors.initials: AT
authors.lastname: Morse
authors.forename: Edward
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authors.lastname: Nigoghossian
authors.forename: Amanda
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authors.lastname: Nopakun
authors.forename: Linda
authors.initials: L
authors.lastname: Okach
authors.forename: Christina
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authors.lastname: Puckett
authors.forename: Ron
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authors.lastname: Reyes
authors.forename: Henry J
authors.initials: HJ
authors.lastname: Tien
authors.forename: Christine B
authors.initials: CB
authors.lastname: Trame
authors.forename: Henry
authors.initials: H
authors.lastname: van den Bedem
authors.forename: Dana
authors.initials: D
authors.lastname: Weekes
authors.forename: Tiffany
authors.initials: T
authors.lastname: Wooten
authors.forename: Qingping
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authors.lastname: Xu
authors.forename: Andrew
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authors.lastname: Yeh
authors.forename: Jiadong
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authors.lastname: Zhou
authors.forename: Keith O
authors.initials: KO
authors.lastname: Hodgson
authors.forename: John
authors.initials: J
authors.lastname: Wooley
authors.forename: Marc-André
authors.initials: MA
authors.lastname: Elsliger
authors.forename: Ashley M
authors.initials: AM
authors.lastname: Deacon
authors.forename: Adam
authors.initials: A
authors.lastname: Godzik
authors.forename: Scott A
authors.initials: SA
authors.lastname: Lesley
authors.forename: Ian A
authors.initials: IA
authors.lastname: Wilson
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry*; D003461:Crystallography; D006801:Humans; D008958:Models, Molecular; D058977:Molecular Sequence Annotation; D008969:Molecular Sequence Data; D009344:Neisseria gonorrhoeae / Q000737:chemistry*; D017434:Protein Structure, Tertiary; D012097:Repressor Proteins / Q000737:chemistry*; D015203:Reproducibility of Results; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein; D017386:Sequence Homology, Amino Acid; D055672:Static Electricity; D040681:Structural Homology, Protein
chemical_list: D001426:Bacterial Proteins; D012097:Repressor Proteins; C490764:SUFU protein, human