In vitro and in vivo interactions of aluminum on NTPDase and AChE activities in lymphocytes of rats.
Al adjuvants are used in vaccines to increase the immune response. NTPDase and AChE play a pivotal role and act in the regulation of the immune system. The effect of Al exposure in vitro and in vivo on NTPDase and AChE activities in the lymphocytes of rats was determined. In vitro, ATP hydrolysis was decreased by 20.4% and 17.3% and ADP hydrolysis was decreased by 36.5% and 34.8%, in groups D and E, respectively, when compared to the control. AChE activity was increased by 157.3%, 152.5%, 74.7% and 90.8% in groups B, C, D, and E, respectively, when compared to the control. In vivo, ATP hydrolysis was increased by 85% and 86% and ADP hydrolysis was increased by 104.2% and 74%, in Al plus citrate and Al groups, respectively, when compared to the control. AChE activity was increased by 50.7% in Al plus citrate and by 28.6% in Al groups, when compared to the control. Our results show that Al exposure both in vitro and in vivo altered NTPDase and AChE activities in lymphocytes. These results may demonstrate the ability of Al to elicit the immune system, where NTPDase and AChE activities can act as purinergic and cholinergic markers in lymphocytes.
Kaizer
Rosilene R
RR
Programa de Pós-Graduação em Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Av. Roraima, 97105-900 Santa Maria, RS, Brazil. rosikaizer@gmail.com
Gutierres
Jessié M
JM
Schmatz
Roberta
R
Spanevello
Rosélia M
RM
Morsch
Vera M
VM
Schetinger
Maria R C
MR
Rocha
João B T
JB
D000110:Acetylcholinesterase / Q000378:metabolism*; D000251:Adenosine Triphosphatases / Q000378:metabolism; D000535:Aluminum / Q000494:pharmacology*; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism*; D001081:Apyrase / Q000378:metabolism*; D002478:Cells, Cultured; D004789:Enzyme Activation / Q000187:drug effects; D058851:GPI-Linked Proteins; D008213:Lymphocyte Activation / Q000187:drug effects*; D008297:Male; D011485:Protein Binding; D051381:Rats; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism*
D015703:Antigens, CD; D058851:GPI-Linked Proteins; D000535:Aluminum; D000110:Acetylcholinesterase; C548246:Ache protein, rat; D000251:Adenosine Triphosphatases; D001081:Apyrase; C067869:CD39 antigen