Protective effect of deferoxamine on experimental spinal cord injury in rat.
OBJECTIVE
To observe the protective effect of deferoxamine on experimental spinal cord injury (SCI) in rats.


METHODS
Sprague-Dawley rats were randomly divided into the following four groups.


METHODS
rats were performed laminectomy only; SCI group: rats were performed laminectomy with SCI; DFO group: rats were injected intraperitoneally a bolus of 100mg/kg deferoxamine after SCI; vehicle group: rats were injected intraperitoneally 0.9% saline after SCI. The SCI of animal model was made by using a modified Allen's method on T(10). Six rats of each group were sacrificed at 4h after injured, and the levels of free iron and malondialdehyde (MDA) of involved spinal cord segments were measured by bleomycin assay and the thiobarbituric acid (TBA) separately. The recovery of function was assessed by Modified Tarlov's scale and inclined plane method at 7, 14, 21d after SCI. The histologic changes of the damaged spinal cord were also examined at 7d after SCI.


RESULTS
Following SCI, the levels of free iron and MDA were increased significantly and the Modified Tarlov's score and inclined plane angles decreased in SCI group and vehicle group. In DFO group, the levels of free iron and MDA were not increased, but the Modified Tarlov's score and inclined plane angles decreased, the histological findings were improved as well.


CONCLUSIONS
Deferoxamine can reduce the levels of free iron and lipid peroxidation, and improve the hind limb functional status of rats with spinal cord injury.
Liu
Jinbo
J
Department of Orthopedics, The First People's Hospital of ChangZhou, ChangZhou, China. jsczljb@yahoo.com.cn
Tang
Tiansi
T
Yang
Huilin
H
D000818:Animals; D003676:Deferoxamine / Q000008:administration & dosage / Q000627:therapeutic use*; D004195:Disease Models, Animal; D006614:Hindlimb / Q000187:drug effects* / Q000473:pathology / Q000503:physiopathology; D007274:Injections, Intraperitoneal; D007796:Laminectomy; D018384:Oxidative Stress; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function / Q000187:drug effects* / Q000502:physiology; D013119:Spinal Cord Injuries / Q000188:drug therapy* / Q000503:physiopathology
D003676:Deferoxamine