A comparative study of Taxol production in liquid and solid-state fermentation with Nigrospora sp. a fungus isolated from Taxus globosa.
OBJECTIVE
To determine in liquid (LF) and solid-state fermentation (SSF) the effect of medium concentration on growth and Taxol produced by Nigrospora sp., a fungus isolated from the Mexican yew.


RESULTS
Nigrospora sp. was grown at different concentrations of the base culture medium M1D, i.e. two (2×), four (4×), six (6×) and eight times (8×) the base concentration. The titres of Taxol determined by competitive inhibition enzyme immunoassay increased with increasing medium concentration in LF and SSF but were higher in SSF in every medium concentration. The Taxol produced in SSF and LF with 8× medium was 221 and 142 ng l(-1) . The SSF gave also higher biomass, growth and sugar utilization than LF in every medium. The growth and sugar consumption were modelled by the logistic and the Pirt models, respectively. However, the Luedeking-Piret model was unsuitable for Taxol.


CONCLUSIONS
The SSF surpassed LF in terms of Taxol, growth and sugar utilization; thus, it has significant advantages over LF.


CONCLUSIONS
This is the first report on Taxol production by SSF and the first contribution to evaluate the influence of the medium on Taxol production in LF and SSF.
Ruiz-Sanchez
J
J
Cinvestav-IPN, Department of Biotechnology and Bioengineering, Av. Instituto Politécnico Nacional 2508, México, D.F., México.
Flores-Bustamante
Z R
ZR
Dendooven
L
L
Favela-Torres
E
E
Soca-Chafre
G
G
Galindez-Mayer
J
J
Flores-Cotera
L B
LB
D001203:Ascomycota / Q000378:metabolism*; D003470:Culture Media; D005285:Fermentation; D007218:Industrial Microbiology / Q000379:methods; D017239:Paclitaxel / Q000096:biosynthesis*; D020946:Taxus / Q000737:chemistry / Q000382:microbiology*
D003470:Culture Media; D017239:Paclitaxel