title: Genetic diversity of Cercospora kikuchii isolates from soybean cultured in Argentina as revealed by molecular markers and cercosporin production.
abstract: Leaf blight and purple seed, caused by the fungal pathogen Cercospora kikuchii (Matsumoto & Tomoyasu) M. W. Gardner are very important diseases of soybean (Glycine max L. Merr.) in Argentina. The aims of this work were: (a) to confirm and to assess the genetic variability among C. kikuchii isolates collected from different soybean growing areas in Santa Fe province using inter simple sequence repeats (ISSR) markers and sequence information from the internal transcribed spacer (ITS) region of rDNA and (b) to analyze the cercosporin production of the regional C. kikuchi isolates in order to assess whether there was any relationship between the molecular profiles and the toxin production. Isolates from different regions in Santa Fe province were studied. The sequence of the ITS regions showed high similarity (99-100%) to the GenBank sequences of C. kikuchii BRCK179 (accession number AY633838). The ISSR markers clustered all the isolates into many groups and cercosporin content was highly variable among isolates. No relationship was observed between ITS region, ISSR groups and origin or cercosporin content. The high degree of genetic variability and cercosporin production among isolates compared in this study characterizes a diverse population of C. kikuchii in the region.
authors.affiliation: Cátedra de Microbiología General, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe, Argentina. mclura@fbcb.unl.edu.ar
authors.forename: María Cristina
authors.initials: MC
authors.lastname: Lurá
authors.forename: María Gabriela
authors.initials: MG
authors.lastname: Latorre Rapela
authors.forename: María Celia
authors.initials: MC
authors.lastname: Vaccari
authors.forename: Roxana
authors.initials: R
authors.lastname: Maumary
authors.forename: Anabel
authors.initials: A
authors.lastname: Soldano
authors.forename: Mónica
authors.initials: M
authors.lastname: Mattio
authors.forename: Ana María
authors.initials: AM
authors.lastname: González
mesh_terms: D001118:Argentina; D001203:Ascomycota / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D001483:Base Sequence; D014644:Genetic Variation; D008969:Molecular Sequence Data; D010569:Perylene / Q000031:analogs & derivatives* / Q000378:metabolism; D010802:Phylogeny; D013025:Glycine max / Q000254:growth & development / Q000382:microbiology*
chemical_list: D010569:Perylene; C026363:cercosporin