Golgi partitioning controls mitotic entry through Aurora-A kinase.
At the onset of mitosis, the Golgi complex undergoes a multistep fragmentation process that is required for its correct partitioning into the daughter cells. Inhibition of this Golgi fragmentation results in cell cycle arrest at the G2 stage, suggesting that correct inheritance of the Golgi complex is monitored by a "Golgi mitotic checkpoint." However, the molecular basis of this G2 block is not known. Here, we show that the G2-specific Golgi fragmentation stage is concomitant with centrosome recruitment and activation of the mitotic kinase Aurora-A, an essential regulator for entry into mitosis. We show that a block of Golgi partitioning impairs centrosome recruitment and activation of Aurora-A, which results in the G2 block of cell cycle progression. Overexpression of Aurora-A overrides this cell cycle block, indicating that Aurora-A is a major effector of the Golgi checkpoint. Our findings provide the basis for further understanding of the signaling pathways that coordinate organelle inheritance and cell duplication.
Persico
Angela
A
Department of Cell Biology and Oncology, Consorzio Mario Negri Sud, 66030 Santa Maria Imbaro, Chieti, Italy.
Cervigni
Romina Ines
RI
Barretta
Maria Luisa
ML
Corda
Daniela
D
Colanzi
Antonino
A
D000818:Animals; D064096:Aurora Kinase A; D064127:Aurora Kinases; D002478:Cells, Cultured; D018385:Centrosome / Q000201:enzymology; D004789:Enzyme Activation; D016195:G2 Phase / Q000502:physiology*; D006056:Golgi Apparatus / Q000201:enzymology*; D006367:HeLa Cells; D006801:Humans; D007668:Kidney / Q000166:cytology; D008938:Mitosis / Q000502:physiology*; D058990:Molecular Targeted Therapy / Q000379:methods; D017346:Protein Serine-Threonine Kinases / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D051381:Rats
C576920:Aurka protein, rat; D064096:Aurora Kinase A; D064127:Aurora Kinases; D017346:Protein Serine-Threonine Kinases