Evaluation of the origin of a sample of Sporothrix schenckii that caused contamination of a researcher in Southern Brazil.
We report a case of a researcher from a laboratory of Mycology in Rio Grande do Sul, Brazil that presented a clinical evidence of sporotrichosis. The researcher had an accident while manipulating the microculture slides of chromoblastomycosis agents and presented a clinical evidence of sporotrichosis. As the laboratory has some cultures of Sporothrix schenckii, it was suggested that it might be a laboratory contamination. In order to test this hypothesis, the genotypic characterization of the samples was performed by means of the random amplified polymorphic DNA (RAPD) analysis method. In addition, we evaluated the in vitro antifungal activity of four antifungal agents against the isolated fungus. The sample obtained from the researcher was not genetically similar to any of the samples kept in the laboratory and showed the minimum inhibitory concentrations of 0.5 μg/mL for itraconazole and ketoconazole, > 64 μg/mL for fluconazole and 0.125 μg/mL for terbinafine. It is suggested that the contamination had an environmental origin.
Landell
Melissa Fontes
MF
Departamento de Microbiologia, ICBS, Universidade Federal do Rio Grande do Sul, Rua Sarmento Leite, 500 Sala 158, Cep: 90.050-170, Porto Alegre, RS, Brazil.
Stopiglia
Cheila Denise Ottonelli
CD
Billodre
Raisa G
RG
Heidrich
Daiane
D
Sorrentino
Julia Medeiros
JM
Vainstein
Marilene H
MH
Scroferneker
Maria Lúcia
ML
Valente
Patricia
P
D001938:Brazil; D015725:Fluconazole / Q000494:pharmacology; D006801:Humans; D007654:Ketoconazole / Q000494:pharmacology; D007753:Laboratories; D008297:Male; D008826:Microbial Sensitivity Tests; D011193:Potassium Iodide / Q000627:therapeutic use; D019105:Random Amplified Polymorphic DNA Technique; D012108:Research Personnel; D013175:Sporothrix / Q000145:classification* / Q000187:drug effects / Q000235:genetics; D013174:Sporotrichosis / Q000175:diagnosis* / Q000188:drug therapy / Q000382:microbiology*
D011193:Potassium Iodide; D015725:Fluconazole; D007654:Ketoconazole