Immunogenicity of recombinant maltose-binding protein (MBP)-gonadotropin releasing hormone I (GnRH-I).
A recombinant fusion protein, maltose-binding protein (MBP)--gonadotropin releasing hormone I (GnRH-I), was produced by cloning a gene fragment encoding a tandem repeated GnRH-I hexamer peptide (GnRH-I6) into the pMAL-c4x vector which was subsequently expressed in E. coli TB1. MBP-GnRH-I6 was affinity purified. MBP-GnRH-I6 was verified by SDS-PAGE and Western blot and immunogenicity tested in boars. Injection of the recombinant fusion protein into boars yielded a high-titer antibody specific for GnRH-I. This was followed by serum testosterone and the degeneration of spermatogenesis. These results showed that MBP-GnRH-I6 acted as a strong immunogen and could be a candidate for an immune antifertility vaccine.
Fang
Fugui
F
Department of Animal Medicine, College of Animal Science and Technology, Anhui Agricultural University, No. 130 Changjiang West Road, Hefei, Anhui, P. R. China.
Liu
Ya
Y
Pu
Yong
Y
Wang
Lin
L
Wang
Suolu
S
Zhang
Xiaorong
X
D000818:Animals; D004926:Escherichia coli / Q000378:metabolism; D007987:Gonadotropin-Releasing Hormone / Q000276:immunology*; D007114:Immunization; D008297:Male; D058619:Maltose-Binding Proteins; D011993:Recombinant Fusion Proteins / Q000276:immunology*; D055101:Semen Analysis; D013091:Spermatogenesis / Q000187:drug effects; D013552:Swine; D013737:Testis / Q000033:anatomy & histology / Q000187:drug effects; D013739:Testosterone / Q000097:blood; D022642:Vaccines, Contraceptive
D058619:Maltose-Binding Proteins; D011993:Recombinant Fusion Proteins; D022642:Vaccines, Contraceptive; C556149:maltose-binding protein-gonadotropin releasing hormone I fusion protein; D007987:Gonadotropin-Releasing Hormone; D013739:Testosterone