title: Analytical performances of the Diazyme ADA assay on the Cobas® 6000 system.
abstract: OBJECTIVE
  To evaluate the analytical performance of the Diazyme ADA assay on the Cobas® 6000 system for pleural fluid samples analysis.
  
  
  METHODS
  Imprecision, linearity, calibration curve stability, interference, and correlation studies were completed.
  
  
  RESULTS
  The Diazyme ADA assay demonstrated excellent precision (CV<4%) over the analytical measurement range (0.5-117 U/L). Bilirubin above 50 μmol/L and haemoglobin above 177 μmol/L interfered with the test, inducing a negative and a positive interference respectively. The Diazyme ADA assay correlated well with the Giusti method (r(2)=0.93) but exhibited a negative bias (~ -30%).
  
  
  CONCLUSIONS
  The Diazyme ADA assay on the Cobas® 6000 system represents a rapid, accurate, precise and reliable method for determination of ADA activity in pleural fluid samples.
authors.affiliation: Hôpital d'Instruction des Armées Bégin, Service de biochimie, Toxicologie, Pharmacologie Cliniques, 69, Saint Mandé, France. h_delacour@yahoo.fr
authors.forename: Hervé
authors.initials: H
authors.lastname: Delacour
authors.forename: Christophe
authors.initials: C
authors.lastname: Sauvanet
authors.forename: Franck
authors.initials: F
authors.lastname: Ceppa
authors.forename: Pascal
authors.initials: P
authors.lastname: Burnat
mesh_terms: D000243:Adenosine Deaminase / Q000032:analysis*; D001681:Biological Assay / Q000295:instrumentation* / Q000379:methods / Q000592:standards; D001826:Body Fluids / Q000201:enzymology*; D002138:Calibration; D006801:Humans; D010996:Pleural Effusion / Q000201:enzymology*
chemical_list: D000243:Adenosine Deaminase