Artefactual effects of oxygen on cell culture models of cellular senescence and stem cell biology.
In life sciences, modelling of the in vivo conditions using in vitro models is an important tool to generate knowledge. Although aerobic organisms including mammals depend on accurate oxygen tension, mimicking physiological conditions in cell culture experiments is not very common. Due to the need for simple technical and experimental design, the requirement for simulating the in vivo oxygen tension parameters has been neglected over long time. Fortunately, due to increasing knowledge in recent years the attention has shifted towards this scientific demand. In this short review, we summarize data substantiating the necessity to adequately mimic physiological oxygen tension using cell culture models in life science research.
Toussaint
Olivier
O
University of Namur/FUNDP, Research Unit on Cellular Biology (URBC), Namur, Belgium.
Weemaels
Geoffroy
G
Debacq-Chainiaux
Florence
F
Scharffetter-Kochanek
Karin
K
Wlaschek
Meinhard
M
D000818:Animals; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D049109:Cell Proliferation; D002470:Cell Survival; D016922:Cellular Senescence; D006801:Humans; D008954:Models, Biological; D010100:Oxygen / Q000378:metabolism*; D055153:Stem Cell Niche; D013234:Stem Cells / Q000166:cytology / Q000502:physiology*
D010100:Oxygen