Regulation of T cell activation and anergy by the intensity of the Ca²+ signal in cooperation with other signals.
The results of our previous in vitro study indicated that the intensity of the Ca²+ signal could determine T cell activation and anergy. We show here that the T cell response of mice that had been treated with cyclosporine A during oral tolerance induction was higher than that of control mice, indicating that the Ca²+ signal could also determine T cell activation and tolerization in vivo. However, T cell activation was not apparent at any concentration of ionomycin, although a low dose of anti-CD3 monoclonal antibody (mAb) induced activation, while a high dose induced anergy in vitro. These results indicate that the balance between the Ca²+ signal and other signals which can also be induced by anti-CD3 stimulation, but not the actual intensity of the Ca²+ signal or the presence of co-stimulation, played an important role in regulating T cell activation and anergy.
Adachi
Yu
Y
Department of Applied Biological Science, Tokyo University of Agriculture and Technology, Tokyo, Japan.
Hattori
Makoto
M
Yoshida
Tadashi
T
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D017252:CD3 Complex / Q000276:immunology; D020013:Calcium Signaling / Q000276:immunology*; D017635:Clonal Anergy / Q000276:immunology*; D016572:Cyclosporine / Q000494:pharmacology; D007166:Immunosuppressive Agents; D015759:Ionomycin / Q000494:pharmacology; D007476:Ionophores; D008213:Lymphocyte Activation / Q000276:immunology*; D051379:Mice; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology*
D000911:Antibodies, Monoclonal; D017252:CD3 Complex; D007166:Immunosuppressive Agents; D007476:Ionophores; D015759:Ionomycin; D016572:Cyclosporine