[Rat kidney mitochondrial damage induced by acute cadmium administration].
OBJECTIVE
To evaluate effect of acute cadmium administration on mitochondria from rat kidney.


METHODS
24 male Sprague-Dawley (SD) rats were randomly divided into four groups. Four groups of rats were injected with saline, 0.6, 1.2, and 1.8 mg/kg weight subcutaneously, once daily, for 5 days, respectively. Ultrastructural change of rat kidney mitochondria was observed, and respiration function, membrane potential, mitochondria swelling, and superoxide level were determined.


RESULTS
Ultrastructural changes included matrix vacuolation, swelling and condensation of mitochondria. In group of 1.8 mg/kg body weight, the oxygen consumption rate during state 3 respiration [(6.25 +/- 0.61) nmol/L O2 x min(-1) x mg(-1)] and RCR value (2.45 +/- 0.23) were significantly lower than those of control group [(9.66 +/- 1.16) nmol/L O2 x min(-1) x mg(-1)] (P < 0.05), indicating respiration inhibition. The membrane potential and superoxide level of the same group were 85.89% +/- 3.82% and 116.33% +/- 3.06% of control values (P < 0.05), respectively.


CONCLUSIONS
Acute cadmium administration can cause rat kidney mitochondrial damage in a dose-effect manner, including inhibition of respiration, dissipation of membrane potential, swelling of mitochondria matrix. Such damage might be related to the increase of mitochondrial free radical.
Pan
Xiao-Hai
XH
School of Public Health/National Key Lab for Public Health Safety of MOE, Fudan University, Shanghai 200032, China.
Sun
Kai
K
Pan
Jia-Hu
JH
Jin
Tai-Yi
TY
D000818:Animals; D002104:Cadmium / Q000633:toxicity*; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D008297:Male; D053078:Membrane Potential, Mitochondrial; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology*; D010100:Oxygen / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism; D023382:Toxicity Tests, Acute
D017382:Reactive Oxygen Species; D002104:Cadmium; D010100:Oxygen