[Different expression of GluR5 and GluR6 in rat astrocyte induced with coriaria lactone and ATPA].
OBJECTIVE
To investigate the different expression of GluR5 and GluR6 in rat astrocyte induced with Coriaria Lactone (CL) and ATPA and to discuss the possible mechanism of the inducing epileptic model.


METHODS
Astrocyte of neonate rats was treated with CL and ATPA respectively and the astrocyte treated with normal saline as control. The mRNA and protein levels of GluR5 and GluR6 were detected by RT-PCR and western blotting.


RESULTS
Compared with the control, the expression of GluR5 was increased significantly in the astrocyte induced by ATPA (P < 0.05), however, there was no alteration of GluR6 expression. The expression of GluR5 and GluR6 were decreased significantly in CL-treated group (P < 0.05).


CONCLUSIONS
The present study showed ATPA up-regulated mRNA and protein levels of GluR5, but coriaria lactone down-regulated the GluR5 and GluR6 of astrocyte. The results indicate that there are different pathologic mechanisms of GluR5 and GluR6 in different neurotoxicants inducing model of epilepsy. The molecular mechanism need to be studied further in the future.
Peng
Fang
F
Department of Neurology, West China Hospital, Sichuan University, Chengdu 610041, China.
Li
Jin-Mei
JM
Li
Qiong
Q
Yao
Zhi-Ping
ZP
Zhou
Dong
D
D000818:Animals; D000831:Animals, Newborn; D001253:Astrocytes / Q000378:metabolism* / Q000473:pathology; D004827:Epilepsy / Q000139:chemically induced* / Q000378:metabolism* / Q000473:pathology; D007555:Isoxazoles / Q000494:pharmacology; D007783:Lactones / Q000494:pharmacology; D011422:Propionates / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D018092:Kainic Acid Receptors / Q000235:genetics / Q000378:metabolism*; D000097806:GluK2 Kainate Receptor
D007555:Isoxazoles; D007783:Lactones; D011422:Propionates; D012333:RNA, Messenger; D018092:Kainic Acid Receptors; D000097806:GluK2 Kainate Receptor; C081662:alpha-amino-3-hydroxy-5-tert-butyl-4-isoxazolepropionate; C049836:coriaria lactone