title: Sugarcane bagasse enzymatic hydrolysis: rheological data as criteria for impeller selection.
abstract: The aim of this work was to select an efficient impeller to be used in a stirred reactor for the enzymatic hydrolysis of sugar cane bagasse. All experiments utilized 100 g (dry weight)/l of steam-pretreated bagasse, which is utilized in Brazil for cattle feed. The process was studied with respect to the rheological behavior of the biomass hydrolysate and the enzymatic conversion of the bagasse polysaccharides. These parameters were applied to model the power required for an impeller to operate at pilot scale (100 l) using empirical correlations according to Nagata [16]. Hydrolysis experiments were carried out using a blend of cellulases, β-glucosidase, and xylanases produced in our laboratory by Trichoderma reesei RUT C30 and Aspergillus awamori. Hydrolyses were performed with an enzyme load of 10 FPU/g (dry weight) of bagasse over 36 h with periodic sampling for the measurement of viscosity and the concentration of glucose and reducing sugars. The mixture presented pseudoplastic behavior. This rheological model allowed for a performance comparison to be made between flat-blade disk (Rushton turbine) and pitched-blade (45°) impellers. The simulation showed that the pitched blade consumed tenfold less energy than the flat-blade disk turbine. The resulting sugar syrups contained 22 g/l of glucose, which corresponded to 45% cellulose conversion.
authors.affiliation: Chemistry Institute, Federal University of Rio de Janeiro (UFRJ), Avenida Athos da Silveira Ramos 149, Bloco A, Cidade Universitária, Rio de Janeiro, RJ, CEP 21941-909, Brazil.
authors.forename: Leonardo Tupi Caldas
authors.initials: LT
authors.lastname: Pereira
authors.forename: Lucas Tupi Caldas
authors.initials: LT
authors.lastname: Pereira
authors.forename: Ricardo Sposina Sobral
authors.initials: RS
authors.lastname: Teixeira
authors.forename: Elba Pinto da Silva
authors.initials: EP
authors.lastname: Bon
authors.forename: Suely Pereira
authors.initials: SP
authors.lastname: Freitas
mesh_terms: D001673:Biodegradation, Environmental; D018533:Biomass; D019149:Bioreactors / Q000382:microbiology; D001938:Brazil; D044602:Cellulases / Q000378:metabolism; D002482:Cellulose / Q000378:metabolism*; D006868:Hydrolysis; D011134:Polysaccharides / Q000378:metabolism; D012212:Rheology; D031786:Saccharum / Q000378:metabolism*; D013227:Steam; D014242:Trichoderma / Q000201:enzymology / Q000378:metabolism; D014783:Viscosity; D018505:Waste Management / Q000295:instrumentation* / Q000379:methods; D001617:beta-Glucosidase / Q000378:metabolism
chemical_list: D011134:Polysaccharides; D013227:Steam; D002482:Cellulose; C027433:bagasse; D044602:Cellulases; D001617:beta-Glucosidase