Highly efficient chemoenzymatic synthesis of β1-3-linked galactosides.
A novel D-galactosyl-β1-3-N-acetyl-D-hexosamine phosphorylase cloned from Bifidobacterium infantis (BiGalHexNAcP) was used with a recombinant E. coli K-12 galactokinase (GalK) for efficient one-pot two-enzyme synthesis of T-antigens, galacto-N-biose (Galβ1-3GalNAc), lacto-N-biose (Galβ1-3GlcNAc), and their derivatives.
Yu
Hai
H
Department of Chemistry, University of California, One Shields Avenue, Davis, California 95616, USA.
Thon
Vireak
V
Lau
Kam
K
Cai
Li
L
Chen
Yi
Y
Mu
Shengmao
S
Li
Yanhong
Y
Wang
Peng George
PG
Chen
Xi
X
D001644:Bifidobacterium / Q000201:enzymology*; D004926:Escherichia coli / Q000201:enzymology*; D005697:Galactosides / Q000737:chemistry / Q000378:metabolism*; D005700:Galactosyltransferases / Q000378:metabolism*; D007218:Industrial Microbiology; D015394:Molecular Structure
D005697:Galactosides; D005700:Galactosyltransferases; C118176:beta-1,3-galactosyl-N-acetylhexosamine phosphorylase