Prior use of the neutral red assay and reduction of total protein determination in 96-well plate assays.
The sequential use of the neutral red and protein assays in 96-well plates leads to approximately a 50% underestimation of the amount of protein. In absolute terms, more protein is lost from wells having more protein, but within a cell type the reduction appears to be proportional to the amount of protein. This should be taken into account when interpreting data obtained in this way.
Arranz
M J
MJ
Medical Research Council Toxicology Unit, Carshalton, Surrey, UK.
Festing
M F
MF