title: Propamidine decreas mitochondrial complex III activity of Botrytis cinerea.
abstract: Propamidine, an aromatic diamidine compound, is widely used as an antimicrobial agent. To uncover its mechanism on pathogenetic fungi, Botrytis cinerea as an object was used to investigate effects of propamidine in this paper. The transmission electron microscope results showed that the mitochondrial membranes were collapsed after propamidine treatment, followed that mitochondria were disrupted. Inhibition of whole-cell and mitochondrial respiration by propamidine suggested that Propamidine is most likely an inhibitor of electron transport within Botrytis cinerea mitochondria. Furthermore, the mitochondrial complex III activity were inhibited by propamidine.
authors.affiliation: Center of Biopesticide Engineering and Technology, Northwest A & F University, Yangling, Shaanxi, China.
authors.forename: Fangli
authors.initials: F
authors.lastname: Wu
authors.forename: Weibo
authors.initials: W
authors.lastname: Jin
authors.forename: Juntao
authors.initials: J
authors.lastname: Feng
authors.forename: Anliang
authors.initials: A
authors.lastname: Chen
authors.forename: Zhiqing
authors.initials: Z
authors.lastname: Ma
authors.forename: Xing
authors.initials: X
authors.lastname: Zhang
mesh_terms: D001550:Benzamidines / Q000494:pharmacology*; D020171:Botrytis / Q000187:drug effects* / Q000378:metabolism* / Q000648:ultrastructure; D004579:Electron Transport / Q000187:drug effects; D014450:Electron Transport Complex III / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D051336:Mitochondrial Membranes / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D001550:Benzamidines; D014450:Electron Transport Complex III; C005555:propamidine