Electrochemical study of spiramycin and its determination in pharmaceutical preparation.
Spiramycin (SPY) is a medium-spectrum antibiotic with high effectiveness against Gram-positive bacteria. The voltammetric behaviour of spiramycin was studied using differential pulse polarography (DPP) and square wave polarography (SWP). The drug in Britton-Robinson buffer (pH 11.5) is reduced at - 1.45 V, giving rise to a well-defined cathodic peak using hanging mercury drop electrode (HMDE) versus Ag/AgCl electrode. This peak is attributed to the reduction of the aldehyde group. The results proved that the reduction of SPY is an irreversible diffusion-controlled process. The diffusion current-concentration relationship was shown to be rectilinear over the range of 20-80 and 0.8-80 µg ml(-1) using DPP and SWP modes, respectively, with detection limit of 8.5 µg ml(-1) (1.01 × 10(-5) M) and 0.46 µg ml(-1) (5.46 × 10(-7) M) for DPP and SWP modes, respectively. A mechanism is postulated for the reduction of SPY. The proposed techniques were successfully applied to the determination of the studied compound either in pure form or in its formulation.
Youssef
Rasha M
RM
Faculty of Pharmacy, Department of Pharmaceutical Analytical Chemistry, University of Alexandria, El-Messalah, Alexandria 21521, Egypt. rmmy1973@yahoo.com
Maher
Hadir M
HM
D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000737:chemistry; D004563:Electrochemistry; D004566:Electrodes; D008628:Mercury / Q000737:chemistry; D011048:Polarography / Q000379:methods*; D018030:Silver Compounds / Q000737:chemistry; D015572:Spiramycin / Q000032:analysis* / Q000737:chemistry
D000900:Anti-Bacterial Agents; D018030:Silver Compounds; D015572:Spiramycin; D008628:Mercury; C037548:silver chloride