Stable, site-specific fluorescent tagging constructs optimized for burkholderia species.
Several vectors that facilitate stable fluorescent labeling of Burkholderia pseudomallei and Burkholderia thailandensis were constructed. These vectors combined the effectiveness of the mini-Tn7 site-specific transposition system with fluorescent proteins optimized for Burkholderia spp., enabling bacterial tracking during cellular infection.
Norris
Michael H
MH
Department of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa, Honolulu, HI 96822, USA.
Kang
Yun
Y
Wilcox
Bruce
B
Hoang
Tung T
TT
D019117:Burkholderia / Q000235:genetics*; D003001:Cloning, Molecular / Q000379:methods; D004251:DNA Transposable Elements; D005453:Fluorescence; D005822:Genetic Vectors; D005827:Genetics, Microbial / Q000379:methods; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008967:Molecular Biology / Q000379:methods; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D013194:Staining and Labeling / Q000379:methods*
D004251:DNA Transposable Elements; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins