Characterization of methane, benzene and toluene-oxidizing consortia enriched from landfill and riparian wetland soils.
The microbial oxidations of methane (M) and volatile organic compounds (VOCs) were compared with those of M and VOCs alone after enriching soil samples with M and/or VOCs. Landfill cover and riparian wetland soils from which M and VOCs were simultaneously emitted were selected as representative samples. Benzene (B) and toluene (T) were employed as the model VOCs. With the landfill soil consortia, the rate of M oxidation decreased from 4.15-5.56 to 2.26-3.42 μmol g-dry soil(-1)h(-1) in the presence of both B and T, but with the wetland soil consortia the rate of M oxidation (3.09 μmol g-dry soil(-1)h(-1)) in the mixture of M as well as both B and T was similar to that of M alone (3.04 μmol g-dry soil(-1)h(-1)). Compared with the methanotrophic community with M alone, the portion of type II methanotrophs was greater in the landfill consortia; whereas, the proportion in wetland consortia was less in the presence of both B and T. The oxidations of B and T were stimulated by the presence of M with both the landfill and wetland consortia. There were no correlations between the oxidation rate of M and those of B and T with the gene copy numbers of pmoA and tmoA responsible for the oxidations.
Lee
Eun-Hee
EH
Department of Environmental Science and Engineering, Ewha Womans University, 11-1 Daehyun-dong, Seodaemun-gu, Seoul 120-750, Republic of Korea.
Park
Hyunjung
H
Department of Environmental Science and Engineering, Ewha Womans University, 11-1 Daehyun-dong, Seodaemun-gu, Seoul 120-750, Republic of Korea.
Cho
Kyung-Suk
KS
Department of Environmental Science and Engineering, Ewha Womans University, 11-1 Daehyun-dong, Seodaemun-gu, Seoul 120-750, Republic of Korea. Electronic address: kscho@ewha.ac.kr.
D001483:Base Sequence; D001554:Benzene / Q000378:metabolism*; D017931:DNA Primers; D008697:Methane / Q000378:metabolism*; D010084:Oxidation-Reduction; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012989:Soil Pollutants / Q000378:metabolism*; D014050:Toluene / Q000378:metabolism*; D053833:Wetlands
D017931:DNA Primers; D012989:Soil Pollutants; D014050:Toluene; D001554:Benzene; D008697:Methane