A cell-to-cell macromolecular transport assay in Planta utilizing biolistic bombardment.
Here, we present a simple and rapid protocol to detect and assess the extent of cell-to-cell macromolecular transport in planta. In this protocol, a fluorescently tagged-protein of interest is transiently expressed in plant tissue following biolistic delivery of its encoding DNA construct. The intra- and intercellular distribution of the tagged protein is then analyzed by confocal microscopy. We describe this technology in detail, providing step-by-step protocols to assay and evaluate the extent of symplastic protein transport in three plant species, Arabidopsis thaliana, Nicotiana benthamiana and N. tabacum (tobacco).
Ueki
Shoko
S
Department of Biochemistry and Cell Biology, SUNY At Stony Brook, USA. sueki@ms.cc.sunysb.edu
Meyers
Benjamin L
BL
Yasmin
Farzana
F
Citovsky
Vitaly
V
D017360:Arabidopsis / Q000166:cytology / Q000378:metabolism; D019470:Biolistics / Q000379:methods*; D002450:Cell Communication / Q000502:physiology*; D018613:Microscopy, Confocal; D046912:Multiprotein Complexes / Q000378:metabolism*; D059828:Plant Cells; D010944:Plants / Q000378:metabolism*; D014026:Nicotiana / Q000166:cytology / Q000378:metabolism
D046912:Multiprotein Complexes