title: Mitochondrial DNA diversity and PCR-based sex determination of Irrawaddy dolphin (Orcaella brevirostris) from Chilika Lagoon, India.
abstract: Of the only known two Lagoon populations of Irrawaddy dolphins (Orcaella) in the world, one is residing in the Chilika Lagoon in Orissa state, India. In addition to accidental deaths in gill net fishery and mechanized boat operations, there has been exploitation of the species for their oil. Extreme patchy distribution and vulnerability to becoming entangled in fishing gear has made it a focus of conservation concern. Information on genetic diversity of populations has considerable potential for informing conservation plans. The present paper reports the first genetic study of O. brevirostris from Chilika Lagoon based on mtDNA sequencing and PCR-based sex identification from 11 individuals. Control region sequence comparison showed two haplotypes and cytochrome b a single haplotype in the Chilika population of the species. Phylogenetic analysis indicated distinct clades within the Asian samples, with the Indian population showing closest genetic proximity to the haplotypes from Thailand. Sex of the animal was determined by PCR-based method. It is important to continue to examine the population discreteness and genetic variation of Irrawaddy dolphin in Chilika Lagoon vis-à-vis its global geographic distribution for formulating the conservation plans of the species.
authors.affiliation: Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar, 751 002 Orissa, India. jayasankarp@vsnl.com
authors.forename: P
authors.initials: P
authors.lastname: Jayasankar
authors.forename: A
authors.initials: A
authors.lastname: Patel
authors.forename: M
authors.initials: M
authors.lastname: Khan
authors.forename: P
authors.initials: P
authors.lastname: Das
authors.forename: S
authors.initials: S
authors.lastname: Panda
mesh_terms: D000818:Animals; D020029:Base Pairing / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics*; D004289:Dolphins / Q000235:genetics*; D005260:Female; D005618:Fresh Water; D014644:Genetic Variation; D006239:Haplotypes / Q000235:genetics; D007194:India; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D012732:Sex Determination Analysis / Q000379:methods*
chemical_list: D004272:DNA, Mitochondrial