title: ADC response to radiation therapy correlates with induced changes in radiosensitivity.
abstract: OBJECTIVE
  Magnetic resonance imaging was used to compare the responses of human glioma tumor xenografts to a single fraction of radiation, where a change in radiosensitivity was induced by use of a suture-based ligature.
  
  
  METHODS
  Ischemia was induced by use of a suture-based ligature. Six mice were treated with 800 cGy of 200 kVp x rays while the ligature was applied. An additional six mice had the ligature applied for the same length of time but were not irradiated. Quantitative maps of each tumor were produced of water apparent diffusion coefficient (ADC) and transverse relaxation time (T2). Mice were imaged before and at multiple points after treatment. Volumetric, ADC, and T2 responses of the ligated groups were compared to previously measured responses of the same tumor model to the same radiation treatment, as well as those from an untreated control group.
  
  
  RESULTS
  Application of the ligature without irradiation did not affect tumor ADC values, but did produce a temporary decrease in tumor T2 values. Average tumor T2 was reduced by 6.2% 24 h after the ligature was applied. Average tumor ADC increased by 9.6% 7 days after irradiation with a ligature applied. This response was significantly less than that observed in the same tumor model when no ligature is present (21.8% at 7 days after irradiation).
  
  
  CONCLUSIONS
  These observations indicate that the response of ADC to radiation therapy is not determined entirely by physical dose deposition, but at least in part by radiosensitivity and resultant biological response.
authors.affiliation: Department of Medical Physics, Cross Cancer Institute, 11560 University Avenue, Edmonton, Alberta T6G 1Z2, Canada.
authors.forename: Matthew P
authors.initials: MP
authors.lastname: Larocque
authors.forename: Alasdair
authors.initials: A
authors.lastname: Syme
authors.forename: Joan
authors.initials: J
authors.lastname: Allalunis-Turner
authors.forename: B Gino
authors.initials: BG
authors.lastname: Fallone
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D004058:Diffusion; D006801:Humans; D051379:Mice; D009369:Neoplasms / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology / Q000532:radiotherapy; D010100:Oxygen / Q000378:metabolism; D011836:Radiation Tolerance / Q000528:radiation effects*; D011878:Radiotherapy; D011879:Radiotherapy Dosage; D013997:Time Factors
chemical_list: D010100:Oxygen