title: Cutaneous C-polymodal fibers lacking TRPV1 are sensitized to heat following inflammation, but fail to drive heat hyperalgesia in the absence of TPV1 containing C-heat fibers.
abstract: BACKGROUND
  Previous studies have shown that the TRPV1 ion channel plays a critical role in the development of heat hyperalgesia after inflammation, as inflamed TRPV1-/- mice develop mechanical allodynia but fail to develop thermal hyperalgesia. In order to further investigate the role of TRPV1, we have used an ex vivo skin/nerve/DRG preparation to examine the effects of CFA-induced-inflammation on the response properties of TRPV1-positive and TRPV1-negative cutaneous nociceptors.
  
  
  RESULTS
  In wildtype mice we found that polymodal C-fibers (CPMs) lacking TRPV1 were sensitized to heat within a day after CFA injection. This sensitization included both a drop in average heat threshold and an increase in firing rate to a heat ramp applied to the skin. No changes were observed in the mechanical response properties of these cells. Conversely, TRPV1-positive mechanically insensitive, heat sensitive fibers (CHs) were not sensitized following inflammation. However, results suggested that some of these fibers may have gained mechanical sensitivity and that some previous silent fibers gained heat sensitivity. In mice lacking TRPV1, inflammation only decreased heat threshold of CPMs but did not sensitize their responses to the heat ramp. No CH-fibers could be identified in naïve nor inflamed TRPV1-/- mice.
  
  
  CONCLUSIONS
  Results obtained here suggest that increased heat sensitivity in TRPV1-negative CPM fibers alone following inflammation is insufficient for the induction of heat hyperalgesia. On the other hand, TRPV1-positive CH fibers appear to play an essential role in this process that may include both afferent and efferent functions.
authors.affiliation: Department of Neurobiology, School of Medicine, University of Pittsburgh, Pittsburgh, PA 15261, USA. rkoerber@pitt.edu
authors.forename: H Richard
authors.initials: HR
authors.lastname: Koerber
authors.forename: Sabrina L
authors.initials: SL
authors.lastname: McIlwrath
authors.forename: Jeffrey J
authors.initials: JJ
authors.lastname: Lawson
authors.forename: Sacha A
authors.initials: SA
authors.lastname: Malin
authors.forename: Collene E
authors.initials: CE
authors.lastname: Anderson
authors.forename: Michael P
authors.initials: MP
authors.lastname: Jankowski
authors.forename: Brian M
authors.initials: BM
authors.lastname: Davis
mesh_terms: D000818:Animals; D006930:Hyperalgesia / Q000378:metabolism* / Q000473:pathology*; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D007249:Inflammation / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D036421:Nerve Fibers, Unmyelinated / Q000378:metabolism* / Q000473:pathology; D009619:Nociceptors / Q000378:metabolism; D012867:Skin / Q000294:innervation* / Q000378:metabolism / Q000473:pathology; D050916:TRPV Cation Channels / Q000172:deficiency* / Q000378:metabolism
chemical_list: D050916:TRPV Cation Channels; C493921:TRPV1 protein, mouse