title: Modulated photophysics of an ESIPT probe 1-hydroxy-2-naphthaldehyde within motionally restricted environments of liposome membranes having varying surface charges.
abstract: The present work demonstrates the modulation of excited state intramolecular proton transfer (ESIPT) emission of 1-hydroxy-2-naphthaldehyde (HN12) upon its interaction with the liposomal vesicles/bilayer of dimyristoyl-l-α-phosphatidylcholine (DMPC) and dimyristoyl-l-α-phosphatidylglycerol (DMPG) and its subsequent implementation as an efficient molecular reporter for probing of microheterogeneous environments of lipid-bilayer system. Modifications on the emission profile of HN12 in terms of remarkable emission intensity enhancement coupled with a hypsochromic shift induced by the presence of DMPC and DMPG membranes have been interpreted to be vivid manifestations of the interactions between the two partners. Steady-state anisotropy, red-edge excitation shift (REES), and time-resolved fluorescence measurements have been fruitfully exploited to complement other experimental findings. Probable binding site of HN12 in the lipid-bilayers has been assessed on the basis of intertwining the results of fluorescence quenching with other experimental results and is further substantiated from docking studies.
authors.affiliation: Department of Chemistry, University of Calcutta, 92 A. P. C. Road, Calcutta-700009, India.
authors.forename: Bijan Kumar
authors.initials: BK
authors.lastname: Paul
authors.forename: Nikhil
authors.initials: N
authors.lastname: Guchhait
mesh_terms: D001665:Binding Sites; D003198:Computer Simulation; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry; D008051:Lipid Bilayers / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry*; D009284:Naphthols / Q000737:chemistry*; D010715:Phosphatidylglycerols / Q000737:chemistry; D011522:Protons; D013050:Spectrometry, Fluorescence
chemical_list: C555036:1-hydroxy-2-naphthaldehyde; D008051:Lipid Bilayers; D008081:Liposomes; D009284:Naphthols; D010715:Phosphatidylglycerols; D011522:Protons; D004134:Dimyristoylphosphatidylcholine