Determination and pharmacokinetics of periplocin in rat plasma by LC-MS.
A simple and sensitive LC-MS method for the determination of periplocin in rat plasma was developed and validated. The chromatographic separation was carried out using a reverse-phase Kromasil C(18) column(150 × 4.6 mm, i.d., 5 µm) with a mobile phase composed of methanol-water (76:24, v/v). The flow rate of mobile phase was 0.8 mL/min. The calibration curve was linear within the concentration range 1-1000 ng/mL. The intra- and inter-day precisions across three validation days over the entire concentration range was lower than 9.2% in terms of relative standard deviation. Accuracy determined at three quality control concentrations ranged from -2.0 to 6.0% in terms of relative error. The validated method was applied to the pharmacokinetic study of periplocin in rat plasma after intravenous and intramuscular administration.
Yi
Lixin
L
School of Pharmacy, Shenyang Pharmaceutical University, Wenhua Road 103, Shenyang, Liaoning 110016, People's Republic of China.
Bi
Kaishun
K
Chen
Xinyang
X
Zhang
Qiufeng
Q
Che
Shuang
S
Liu
Wentao
W
Lu
Dan
D
Chen
Xiaohui
X
D000704:Analysis of Variance; D000818:Animals; D019540:Area Under Curve; D002853:Chromatography, Liquid / Q000379:methods*; D004077:Digoxin / Q000032:analysis / Q000737:chemistry; D004355:Drug Stability; D016014:Linear Models; D008297:Male; D000432:Methanol; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012503:Saponins / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D012503:Saponins; C502381:periplocin; D004077:Digoxin; D000432:Methanol