Enzyme immobilisation on self-organised nanopatterned electrode surfaces.
A new method is described for immobilisation of enzymes on polymer-coated Pt islands. These islands are deposited on top of a SAM-covered Au(111) electrode by a combination of electroless and electrochemical deposition, which allows for a variation of island size and distance between the islands. Here we describe the immobilisation of pyranose-2-oxidase (P2Ox) and the catalytic response to D-glucose on such a nanopatterned surface, which provides optimum access to the active centres of the enzyme.
Gajdzik
Janine
J
Physical Chemistry, Saarland University, 66123 Saarbrücken, Germany.
Lenz
Jennifer
J
Natter
Harald
H
Hempelmann
Rolf
R
Kohring
Gert-Wieland
GW
Giffhorn
Friedrich
F
Manolova
Mila
M
Kolb
Dieter M
DM
D015374:Biosensing Techniques / Q000379:methods*; D002237:Carbohydrate Dehydrogenases / Q000737:chemistry / Q000378:metabolism*; D004563:Electrochemistry / Q000379:methods; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D049329:Nanostructures / Q000737:chemistry / Q000648:ultrastructure; D010984:Platinum / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*
D004800:Enzymes, Immobilized; D011108:Polymers; D010984:Platinum; D002237:Carbohydrate Dehydrogenases; C022489:pyranose oxidase; D005947:Glucose