title: Telomerase recruitment in Saccharomyces cerevisiae is not dependent on Tel1-mediated phosphorylation of Cdc13.
abstract: In Saccharomyces cerevisiae, association between the Est1 telomerase subunit and the telomere-binding protein Cdc13 is essential for telomerase to be recruited to its site of action. A current model proposes that Tel1 binding to telomeres marks them for elongation, as the result of phosphorylation of a proposed S/TQ cluster in the telomerase recruitment domain of Cdc13. However, three observations presented here argue against one key aspect of this model. First, the pattern of Cdc13 phosphatase-sensitive isoforms is not altered by loss of Tel1 function or by mutations introduced into two conserved serines (S249 and S255) in the Cdc13 recruitment domain. Second, an interaction between Cdc13 and Est1, as monitored by a two-hybrid assay, is dependent on S255 but Tel1-independent. Finally, a derivative of Cdc13, cdc13-(S/TQ)11→(S/TA)11, in which every potential consensus phosphorylation site for Tel1 has been eliminated, confers nearly wild-type telomere length. These results are inconsistent with a model in which the Cdc13-Est1 interaction is regulated by Tel1-mediated phosphorylation of the Cdc13 telomerase recruitment domain. We propose an alternative model for the role of Tel1 in telomere homeostasis, which is based on the assumption that Tel1 performs the same molecular task at double-strand breaks (DSBs) and chromosome termini.
authors.affiliation: Salk Institute for Biological Studies, La Jolla, California 92093-0130, USA.
authors.forename: Hua
authors.initials: H
authors.lastname: Gao
authors.forename: Tasha B
authors.initials: TB
authors.lastname: Toro
authors.forename: Margherita
authors.initials: M
authors.lastname: Paschini
authors.forename: Bari
authors.initials: B
authors.lastname: Braunstein-Ballew
authors.forename: Rachel B
authors.initials: RB
authors.lastname: Cervantes
authors.forename: Victoria
authors.initials: V
authors.lastname: Lundblad
mesh_terms: D006487:Hemostasis; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism* / Q000502:physiology; D010766:Phosphorylation; D011485:Protein Binding; D020033:Protein Isoforms; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism* / Q000502:physiology; D021381:Protein Transport; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism* / Q000502:physiology; D019098:Telomerase / Q000378:metabolism*; D016615:Telomere; D034501:Telomere-Binding Proteins / Q000378:metabolism*
chemical_list: C452219:Cdc13 protein, S cerevisiae; D047908:Intracellular Signaling Peptides and Proteins; D020033:Protein Isoforms; D029701:Saccharomyces cerevisiae Proteins; D034501:Telomere-Binding Proteins; D017346:Protein Serine-Threonine Kinases; C513623:TEL1 protein, S cerevisiae; C099024:EST1 protein, S cerevisiae; D019098:Telomerase