title: Modifications of a calcium phosphate cement with biomolecules--influence on nanostructure, material, and biological properties.
abstract: Calcium phosphate cements (CPC), forming hydroxyapatite during the setting reaction, are characterized by good biocompatibility and osteoconductivity, however, their remodeling into native bone tissue is slow. One strategy to improve remodeling and bone regeneration is the directed modification of their nanostructure. In this study, a CPC was set in the presence of cocarboxylase, glucuronic acid, tartaric acid, α-glucose-1-phosphate, L-arginine, L-aspartic acid, and L-lysine, respectively, with the aim to influence formation and growth of hydroxyapatite crystals through the functional groups of these biomolecules. Except for glucuronic acid, all these modifications resulted in the formation of smaller and more agglomerated hydroxyapatite particles which had a positive impact on the biological performance indicated by first experiments with the human osteoblast cell line hFOB 1.19. Moreover, adhesion, proliferation, and osteogenic differentiation of human bone marrow-derived mesenchymal stem cells (hBMSC) as well as binding of the growth factors BMP-2 and VEGF was investigated on CPC modified with cocarboxylase, arginine, and aspartic acid. Initial adhesion of hBMSC was improved on these three modifications and proliferation was enhanced on CPC modified with cocarboxylase and arginine whereas osteogenic differentiation remained unaffected. Modification of the CPC with arginine and aspartic acid, but not with cocarboxylase, led to a higher BMP-2 binding.
authors.affiliation: Max Bergmann Center of Biomaterials and Institute for Materials Science, Technische Universität Dresden, Budapester Str 27, D-01069 Dresden, Germany.
authors.forename: Corina
authors.initials: C
authors.lastname: Vater
authors.forename: Anja
authors.initials: A
authors.lastname: Lode
authors.forename: Anne
authors.initials: A
authors.lastname: Bernhardt
authors.forename: Antje
authors.initials: A
authors.lastname: Reinstorf
authors.forename: Berthold
authors.initials: B
authors.lastname: Nies
authors.forename: Michael
authors.initials: M
authors.lastname: Gelinsky
mesh_terms: D001120:Arginine / Q000737:chemistry / Q000378:metabolism; D001224:Aspartic Acid / Q000737:chemistry / Q000378:metabolism; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism; D001843:Bone Cements / Q000737:chemistry*; D055396:Bone Morphogenetic Protein 2 / Q000378:metabolism; D001861:Bone Regeneration / Q000502:physiology; D002130:Calcium Phosphates / Q000737:chemistry*; D002454:Cell Differentiation; D002460:Cell Line; D049109:Cell Proliferation; D006801:Humans; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology; D015394:Molecular Structure; D049329:Nanostructures / Q000737:chemistry*; D010006:Osteoblasts / Q000166:cytology / Q000502:physiology; D013835:Thiamine Pyrophosphate / Q000737:chemistry / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D001672:Biocompatible Materials; D001843:Bone Cements; D055396:Bone Morphogenetic Protein 2; D002130:Calcium Phosphates; D042461:Vascular Endothelial Growth Factor A; D001224:Aspartic Acid; D001120:Arginine; D013835:Thiamine Pyrophosphate