title: Tetrahydropalmatine inhibits pro-inflammatory mediators in lipopolysaccharide-stimulated THP-1 cells.
abstract: For centuries, Corydalis Rhizoma has been used in Korean traditional medicine. Tetrahydropalmatine is an alkaloid compound and a prominent anti-inflammatory agent found in plants, including Corydalis yanhusuo. However, the therapeutic mechanisms of tetrahydropalmatine are still not well understood. To provide insight into the biological effects of tetrahydropalmatine, we examined its influence on lipopolysaccharide (LPS)-induced interleukin (IL)-8 production in the human monocytic cell line THP-1. In the present study, IL-8 production was measured using enzyme-linked immunosorbent assay, reverse transcription-polymerase chain reaction, and mitogen-activated protein kinase (MAPK) activation, as determined by western blot analysis. Tetrahydropalmatine inhibited LPS-induced IL-8 production in a dose-dependent manner. Furthermore, tetrahydropalmatine inhibited extracellular signal-regulated kinase and p38 MAPK phosphorylation, which suggests that tetrahydropalmatine inhibits IL-8 secretion by blocking MAPK phosphorylation. Taken together, these findings may help elucidate the mechanism by which tetrahydropalmatine modulates THP-1 cell activation under inflammatory conditions.
authors.affiliation: Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University Wonkwang Oriental Medicines Research Institute, Iksan, Republic of Korea.
authors.forename: You-Chang
authors.initials: YC
authors.lastname: Oh
authors.forename: Jang-Gi
authors.initials: JG
authors.lastname: Choi
authors.forename: Young-Seob
authors.initials: YS
authors.lastname: Lee
authors.forename: Obiang-Obounou
authors.initials: OO
authors.lastname: Brice
authors.forename: Soo-Chan
authors.initials: SC
authors.lastname: Lee
authors.forename: Hwa-Sun
authors.initials: HS
authors.lastname: Kwak
authors.forename: Young-Ho
authors.initials: YH
authors.lastname: Byun
authors.forename: Ok-Hwa
authors.initials: OH
authors.lastname: Kang
authors.forename: Jung-Rae
authors.initials: JR
authors.lastname: Rho
authors.forename: Dong-Won
authors.initials: DW
authors.lastname: Shin
authors.forename: Dong-Yeul
authors.initials: DY
authors.lastname: Kwon
mesh_terms: D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D001600:Berberine Alkaloids / Q000494:pharmacology*; D002460:Cell Line; D031311:Corydalis / Q000737:chemistry; D048049:Extracellular Signal-Regulated MAP Kinases / Q000037:antagonists & inhibitors; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D020672:I-kappa B Proteins / Q000187:drug effects / Q000378:metabolism; D016209:Interleukin-8 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology*; D009000:Monocytes / Q000187:drug effects* / Q000378:metabolism*; D000072000:NF-KappaB Inhibitor alpha; D016328:NF-kappa B / Q000037:antagonists & inhibitors; D010766:Phosphorylation / Q000187:drug effects; D012333:RNA, Messenger / Q000032:analysis; D048051:p38 Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors
chemical_list: D000893:Anti-Inflammatory Agents; D001600:Berberine Alkaloids; D020672:I-kappa B Proteins; D016209:Interleukin-8; D008070:Lipopolysaccharides; D016328:NF-kappa B; C000606204:NFKBIA protein, human; D012333:RNA, Messenger; D000072000:NF-KappaB Inhibitor alpha; C014215:tetrahydropalmatine; D048049:Extracellular Signal-Regulated MAP Kinases; D048051:p38 Mitogen-Activated Protein Kinases