Cardiac troponin T detection using polymers coated quartz crystal microbalance as a cost-effective immunosensor.
Cardiac troponin T (cTnT) detection has been the focus of increased interest due to its role in myocardial infarction diagnosis. In this study, we report a relatively low coat technique to detect cTnT using a quartz crystal microbalance (QCM) sensor. A sensitive detection is achieved by introducing a QCM surface with a carboxylic polyvinyl chloride immobilization layer. The surface morphologies of this polymer film under varied deposition thickness have been investigated by field emission scanning electron microscopy and atomic force microscopy. A cTnT detection result from a modified QCM surface can be obtained within a short response time by a direct detection of the immunoreaction and a direct conversion of mass accumulation into a frequency shift, representing a measurable electrical signal. The relationship between the cTnT concentration and the response current from a QCM sensor shows detectability at the concentration of cTnT as low as 5 ng/ml.
Wong-ek
Krongkamol
K
Nanoscience and Technology Program, Chulalongkorn University, Bangkok, Thailand. wongek@tu.ac.th
Chailapakul
Orawon
O
Nuntawong
Noppadon
N
Jaruwongrungsee
Kata
K
Tuantranont
Adisorn
A
D015374:Biosensing Techniques / Q000295:instrumentation*; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007118:Immunoassay / Q000295:instrumentation*; D055617:Micro-Electrical-Mechanical Systems / Q000295:instrumentation*; D011108:Polymers / Q000737:chemistry; D011791:Quartz / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D020107:Troponin T / Q000097:blood*
D020099:Coated Materials, Biocompatible; D011108:Polymers; D020107:Troponin T; D011791:Quartz