title: Microarray-based detection of virulence genes in verotoxigenic Escherichia coli O157:H7 strains from Swedish cattle.
abstract: Verotoxigenic Escherichia coli (VTEC) serotype O157:H7 strains from a Swedish cattle prevalence study (n=32), and livestock-derived strains linked to human disease (n=13), were characterized by microarray and PCR detection of virulence genes. The overall aim of the study was to investigate the distribution of known virulence determinants and determine which genes are linked to increased pathogenicity in humans. A core set of 18 genes or gene variants were found in all strains, while seven genes were variably present. This suggests that the majority of VTEC O157:H7 found in Swedish cattle carry a broad repertoire of virulence genes and should be considered potentially harmful to humans. A single virulence gene type was significantly associated with strains linked to human disease cases (P=0.012), but no genetic trait to explain the increased virulence of this genotype could be found.
authors.affiliation: National Veterinary Institute, Uppsala, Sweden.
authors.forename: R
authors.initials: R
authors.lastname: Söderlund
authors.affiliation: National Veterinary Institute, Uppsala, Sweden.
authors.forename: A
authors.initials: A
authors.lastname: Aspán
authors.affiliation: Department for Food and Environmental Safety, Veterinary Laboratories Agency, Addlestone, Surrey, UK.
authors.forename: R M
authors.initials: RM
authors.lastname: La Ragione
authors.affiliation: National Veterinary Institute, Uppsala, Sweden.
authors.forename: E
authors.initials: E
authors.lastname: Eriksson
authors.affiliation: Department of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences, Uppsala, Sweden.
authors.forename: S
authors.initials: S
authors.lastname: Boqvist
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology* / Q000382:microbiology; D016521:Electrophoresis, Gel, Pulsed-Field; D004927:Escherichia coli Infections / Q000382:microbiology / Q000662:veterinary*; D019453:Escherichia coli O157 / Q000235:genetics* / Q000472:pathogenicity; D005798:Genes, Bacterial / Q000235:genetics; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods / Q000662:veterinary*; D016133:Polymerase Chain Reaction; D015995:Prevalence; D013548:Sweden / Q000453:epidemiology; D014774:Virulence / Q000235:genetics