Effects of chitooligosaccharide lactate salt on activity of acetaldehyde dehydrogenase.
Chitooligosaccharides (COS), a kind of oligosaccharide made from chitin or chitosan, have been used a popular remedy for hangovers. In this study we investigated the in vitro effect of COS lactate salt on ethanol-induced cytotoxicity and the in vivo effect of short-term COS lactate salt feeding on ethanol-induced hangover. Pretreatment of HepG2 cells with COS lactate salt significantly reduced ethanol-induced cytotoxicity and suppressed generation of reactive oxygen species. In addition, COS lactate salt dose-dependently increased acetaldehyde dehydrogenase (ALDH) activity in vitro and reversed the ALDH inhibition induced by daidzin. Furthermore, oral administration of COS lactate salt (200 mg/kg) for 5 days significantly decreased the blood levels of alcohol and acetaldehyde in ethanol-treated mice. It was also demonstrated that hepatic mitochondrial ALDH activity was significantly increased in COS lactate salt-treated mice. Taken together, these findings indicate that COS lactate salt may have efficacy for the management of alcoholic hangovers.
Cho
Si Young
SY
Food Research Institute, R&D Center, AmorePacific Corporation, Giheung-gu, Gyeonggi-do, Republic of Korea.
Yun
Jun-Won
JW
Park
Pil Joon
PJ
Sohn
Jong Hee
JH
Seo
Dae-Bang
DB
Lim
Kyung Min
KM
Kim
Wan Gi
WG
Lee
Sang-Jun
SJ
D000079:Acetaldehyde / Q000097:blood; D000426:Alcohol Dehydrogenase / Q000378:metabolism; D000444:Aldehyde Dehydrogenase / Q000235:genetics; D000071396:Aldehyde Dehydrogenase, Mitochondrial; D000445:Aldehyde Oxidoreductases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D000431:Ethanol / Q000097:blood / Q000633:toxicity; D056945:Hep G2 Cells; D006801:Humans; D007529:Isoflavones / Q000494:pharmacology; D007773:Lactates / Q000494:pharmacology*; D008099:Liver / Q000201:enzymology*; D008297:Male; D051379:Mice; D008812:Mice, Hairless; D008930:Mitochondria, Liver / Q000201:enzymology; D009844:Oligosaccharides / Q000494:pharmacology*; D012333:RNA, Messenger / Q000032:analysis; D017382:Reactive Oxygen Species / Q000378:metabolism
D004791:Enzyme Inhibitors; D007529:Isoflavones; D007773:Lactates; D009844:Oligosaccharides; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D000431:Ethanol; C013908:daidzin; D048271:Chitosan; D000426:Alcohol Dehydrogenase; D000445:Aldehyde Oxidoreductases; C515029:ALDH2 protein, mouse; D000444:Aldehyde Dehydrogenase; D000071396:Aldehyde Dehydrogenase, Mitochondrial; C018699:aldehyde dehydrogenase (NAD(P)+); D000079:Acetaldehyde