title: Advances in methods for the determination of biologically relevant lipid peroxidation products.
abstract: Lipid peroxidation is recognized to be an important contributor to many chronic diseases, especially those of an inflammatory pathology. In addition to their value as markers of oxidative damage, lipid peroxidation products have also been shown to have a wide variety of biological and cell signalling effects. In view of this, accurate and sensitive methods for the measurement of lipid peroxidation products are essential. Although some assays have been described for many years, improvements in protocols are continually being reported and, with recent advances in instrumentation and technology, highly specialized and informative techniques are increasingly used. This article gives an overview of the most currently used methods and then addresses the recent advances in some specific approaches. The focus is on analysis of oxysterols, F(2)-isoprostanes and oxidized phospholipids by gas chromatography or liquid chromatography mass spectrometry techniques and immunoassays for the detection of 4-hydroxynonenal.
authors.affiliation: Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, UK. c.m.spickett@strath.ac.uk
authors.forename: Corinne M
authors.initials: CM
authors.lastname: Spickett
authors.forename: Ingrid
authors.initials: I
authors.lastname: Wiswedel
authors.forename: Werner
authors.initials: W
authors.lastname: Siems
authors.forename: Kamelija
authors.initials: K
authors.lastname: Zarkovic
authors.forename: Neven
authors.initials: N
authors.lastname: Zarkovic
mesh_terms: D000447:Aldehydes / Q000032:analysis*; D000818:Animals; D019963:Clinical Chemistry Tests / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay; D028441:F2-Isoprostanes / Q000032:analysis*; D006801:Humans; D007150:Immunohistochemistry; D015227:Lipid Peroxidation; D013058:Mass Spectrometry; D018384:Oxidative Stress / Q000502:physiology*
chemical_list: D000447:Aldehydes; D028441:F2-Isoprostanes; C027576:4-hydroxy-2-nonenal