Integration of bovine herpesvirus 4 genome into cultured persistently infected host cell genome.
Persistent infection of macrophages with bovine herpesvirus 4 (BoHV-4) has been proposed to play a secondary causal role, along with bacterial infection, in bovine post-partum metritis. Mechanisms of maintenance of BoHV-4 persistent infection are not understood. We previously generated in vitro models of BoHV-4 persistent infection in human rhadomyosarcoma and bovine macrophage cell lines by drug selection of cells infected with BoHV-4 carrying a drug-resistance marker, and demonstrated circular episomal BoHV-4 genomes. In the present study, we used fluorescent in situ hybridization (FISH) to demonstrate BoHV-4 genomes also integrated into the genomes of these persistently infected cells.
Donofrio
Gaetano
G
Dipartimento di Salute Animale, Sezione di Malattie Infettive degli Animali, Università di Parma, Via del Taglio 10, 43100 Parma, Italy. gaetano.donofrio@unipr.it
Capocefalo
Antonio
A
Franceschi
Valentina
V
De Lorenzi
Lisa
L
van Santen
Vicky
V
Parma
Pietro
P
D000818:Animals; D002417:Cattle; D002460:Cell Line; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D026021:Herpesvirus 4, Bovine / Q000235:genetics / Q000502:physiology*; D017404:In Situ Hybridization, Fluorescence; D016662:Virus Integration
D004279:DNA, Viral