Effect of 60 Hz magnetic fields on the activation of hsp70 promoter in cultured INER-37 and RMA E7 cells.
It has been reported that 50-60 Hz magnetic fields (MF) with flux densities ranging from microtesla to millitesla are able to induce heat shock factor or heat shock proteins in various cells. In this study, we investigated the effect of 60 Hz sinusoidal MF at 8 and 80 μT on the expression of the luciferase gene contained in a plasmid labeled as electromagnetic field-plasmid (pEMF). This gene construct contains the specific sequences previously described for the induction of hsp70 expression by MF, as well as the reporter for the luciferase gene. The pEMF vector was transfected into INER-37 and RMA E7 cell lines that were later exposed to either MF or thermal shock (TS). Cells that received the MF or TS treatments and their controls were processed according to the luciferase assay system for evaluate luciferase activity. An increased luciferase gene expression was observed in INER-37 cells exposed to MF and TS compared with controls (p < 0.05), but MF exposure had no effect on the RMA E7 cell line.
Heredia-Rojas
J Antonio
JA
Departamento de Ciencias Exactas y Desarrollo Humano, Facultad de Ciencias Biológicas, UANL, Serafín Peña #909 Norte, Monterrey, Nuevo León C.P. 64000, Mexico. jherediarojas@gmail.com
Rodríguez de la Fuente
Abraham Octavio
AO
Alcocer González
Juan Manuel
JM
Rodríguez-Flores
Laura E
LE
Rodríguez-Padilla
Cristina
C
Santoyo-Stephano
Martha A
MA
Castañeda-Garza
Esperanza
E
Taméz-Guerra
Reyes S
RS
D000818:Animals; D045744:Cell Line, Tumor; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*; D006801:Humans; D008156:Luciferases / Q000235:genetics*; D008280:Magnetics / Q000379:methods*; D051379:Mice; D011401:Promoter Regions, Genetic / Q000235:genetics / Q000502:physiology*; D015533:Transcriptional Activation; D014162:Transfection
D018840:HSP70 Heat-Shock Proteins; D008156:Luciferases