title: Importin β3 mediates the nuclear import of human ribosomal protein L7 through its interaction with the multifaceted basic clusters of L7.
abstract: We show that importin β3 is essential for the nuclear import of L7. The import is mediated via the multifaceted basic amino acid clusters present in the NH(2)-region of L7, and is RanGTP-dependent. Using a (EGFP)(3) reporter system and a FRAP assay, the role the individual clusters play as a functional NLS has been characterized, and each cluster was found to exhibit a different rate of real time nuclear uptake. We assume that having such a multiple NLS may provide L7 with preferential nuclear uptake.
authors.affiliation: Institute of Genome Sciences, School of Life Sciences, National Yang-Ming University, Shih-Pai, Taipei, Taiwan, ROC.
authors.forename: Chang-Wei
authors.initials: CW
authors.lastname: Chou
authors.forename: Lin-Ru
authors.initials: LR
authors.lastname: Tai
authors.forename: Ralph
authors.initials: R
authors.lastname: Kirby
authors.forename: I-Fang
authors.initials: IF
authors.lastname: Lee
authors.forename: Alan
authors.initials: A
authors.lastname: Lin
mesh_terms: D021581:Active Transport, Cell Nucleus; D000595:Amino Acid Sequence; D001665:Binding Sites; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D055785:Gene Knockdown Techniques; D049452:Green Fluorescent Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D050505:Mutant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D011485:Protein Binding; D054730:Protein Interaction Domains and Motifs; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012269:Ribosomal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D028961:beta Karyopherins / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020931:ran GTP-Binding Protein / Q000378:metabolism
chemical_list: D002352:Carrier Proteins; C107940:IPO5 protein, human; D050505:Mutant Proteins; C053634:RPL7 protein, human; D011994:Recombinant Proteins; D012269:Ribosomal Proteins; D028961:beta Karyopherins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D020931:ran GTP-Binding Protein