title: Comparison of the effect of oligonol, a new lychee fruit-derived low molecular form of polyphenol, and epigallocatechin-3-gallate on lipolysis in rat primary adipocytes.
abstract: Several polyphenols have been shown to be beneficial in preventing the accumulation of body fat in mammals. This paper reports that adding oligonol, a lychee fruit-derived low-molecular form of polyphenol mixture, has a greater effect on lipolysis in primary adipocytes compared with tea (-)-epigallocatechin-3-gallate (EGCG) alone, accompanied by a significant increase in activation of extracellular signalling-related kinase 1/2 (ERK1/2). However, neither phosphorylation of mitogen-activated protein kinase 1/2 (MEK1/2), a molecule upstream of ERK1/2, nor the level of heme oxygenase-1 (HO-1), a molecule downstream of ERK1/2 was significantly changed between oligonol and EGCG, although the addition of oligonol and EGCG significantly increased the levels of phosphorylated MEK1/2 and HO-1 compared with the non-treated control cells. These results suggest that the coordinated direct effect of mixed polyphenol, which comprises oligonol, on ERK1/2 plays a key role in a greater lipolytic response to oligonol than EGCG alone.
authors.affiliation: Department of Molecular Predictive Medicine and Sport Science, Kyorin University, School of Medicine, Mitaka, Tokyo 181-8611, Japan. junetsu@ks.kyorin-u.ac.jp
authors.forename: Junetsu
authors.initials: J
authors.lastname: Ogasawara
authors.forename: Kentaro
authors.initials: K
authors.lastname: Kitadate
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nishioka
authors.forename: Hajime
authors.initials: H
authors.lastname: Fujii
authors.forename: Takuya
authors.initials: T
authors.lastname: Sakurai
authors.forename: Takako
authors.initials: T
authors.lastname: Kizaki
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Izawa
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Ishida
authors.forename: Hideki
authors.initials: H
authors.lastname: Ohno
mesh_terms: D017667:Adipocytes / Q000187:drug effects*; D000818:Animals; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D002478:Cells, Cultured; D005638:Fruit / Q000737:chemistry; D051547:Heme Oxygenase-1 / Q000378:metabolism; D008066:Lipolysis / Q000187:drug effects*; D032125:Litchi / Q000737:chemistry*; D008297:Male; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D010636:Phenols / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D010636:Phenols; C514283:oligonol; D002392:Catechin; C045651:epigallocatechin gallate; D051547:Heme Oxygenase-1; D020928:Mitogen-Activated Protein Kinases