A TRAP transporter for pyruvate and other monocarboxylate 2-oxoacids in the cyanobacterium Anabaena sp. strain PCC 7120.
In the cyanobacterium Anabaena sp. strain PCC 7120, open reading frames (ORFs) alr3026, alr3027, and all3028 encode a tripartite ATP-independent periplasmic transporter (TRAP-T). Wild-type filaments showed significant uptake of [(14)C]pyruvate, which was impaired in the alr3027 and all3028 mutants and was inhibited by several monocarboxylate 2-oxoacids, identifying this TRAP-T system as a pyruvate/monocarboxylate 2-oxoacid transporter.
Pernil
Rafael
R
Instituto de Bioquímica Vegetal y Fotosíntesis, Consejo Superior de Investigaciones Científicas, Universidad de Sevilla, Seville, Spain.
Herrero
Antonia
A
Flores
Enrique
E
D017033:Anabaena / Q000378:metabolism*; D002250:Carbon Radioisotopes / Q000378:metabolism; D002264:Carboxylic Acids / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000378:metabolism; D017353:Gene Deletion; D026901:Membrane Transport Proteins / Q000378:metabolism*; D016254:Mutagenesis, Insertional; D019289:Pyruvic Acid / Q000378:metabolism*; D013194:Staining and Labeling
D002250:Carbon Radioisotopes; D002264:Carboxylic Acids; D004791:Enzyme Inhibitors; D026901:Membrane Transport Proteins; D019289:Pyruvic Acid