title: [Studies about the level of CD4+ CD25+ regulatory T cells and relation between expression of Foxp3 and CD127 in peripheral blood of chronic HBV infection].
abstract: OBJECTIVE
  To investigate the level of CD4+ CD25+ Foxp3+ regulatory T cells and observe relation between expression of Foxp3 and CD127 in peripheral blood of chronic HBV infection.
  
  
  METHODS
  CD4+ CD25+ Foxp3+ and CD4+ CD25+ CD127low Treg in peripheral blood from 34 patients of immune tolerance stage, 26 patients of immune clearance stage and 31 patients of non-active status were quantitatively analyzed by flow cytometry.
  
  
  RESULTS
  Immune tolerance group presented a higher fraction of CD4+ CD25+ Foxp3+ and CD4+ CD25+ CD127low Treg than non-active group in chronic HBV infection (Z = -2.693, P = 0.007 and t = 3.251, P = 0.002), and HBV positive group also presented a higher fraction than non-active group (t = 2.266, P = 0.026 and t = 3.208, P = 0.002), But ALT normal group is similar to ALT abnormal group (P > 0.05). In this study, the relation between expression of CD127low and Foxp3+ from CD4+ CD25+ regulatory T cells was observed, and CD4+ CD25+ CD127low Treg presented a higher fraction than CD4+ CD25+ Foxp3+ Treg.
  
  
  CONCLUSIONS
  Peripheral Treg in HBV active replication group is higher than HBV negative group of chronic HBV infection. Expression of CD127low is consistent with Foxp3+ in CD4+ CD25+ regulatory T cells, but the former is significantly higher than the latter.
authors.affiliation: Department of Infectious Diseases, The first Affiliated Hospital of Nanchang University, Nanchang 330006, China.
authors.forename: Min
authors.initials: M
authors.lastname: Deng
authors.forename: Ming-Hui
authors.initials: MH
authors.lastname: Li
authors.forename: Shun-Ai
authors.initials: SA
authors.lastname: Liu
authors.forename: Feng
authors.initials: F
authors.lastname: Liu
authors.forename: Yan
authors.initials: Y
authors.lastname: Sang
authors.forename: Shu-Jing
authors.initials: SJ
authors.lastname: Song
authors.forename: Shu-Feng
authors.initials: SF
authors.lastname: Zang
authors.forename: Xiao-Ping
authors.initials: XP
authors.lastname: Guan
authors.forename: Xie
authors.initials: X
authors.lastname: Yao
authors.forename: Xiao-Ping
authors.initials: XP
authors.lastname: Wu
authors.forename: Jun
authors.initials: J
authors.lastname: Cheng
authors.forename: Dao-Zhen
authors.initials: DZ
authors.lastname: Xu
mesh_terms: D000293:Adolescent; D000328:Adult; D005260:Female; D051858:Forkhead Transcription Factors / Q000097:blood / Q000235:genetics* / Q000276:immunology; D006515:Hepatitis B virus / Q000276:immunology*; D019694:Hepatitis B, Chronic / Q000235:genetics* / Q000276:immunology* / Q000821:virology; D006801:Humans; D053645:Interleukin-2 Receptor alpha Subunit / Q000097:blood / Q000276:immunology*; D053699:Interleukin-7 Receptor alpha Subunit / Q000097:blood / Q000235:genetics* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D055815:Young Adult
chemical_list: C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors; D053645:Interleukin-2 Receptor alpha Subunit; D053699:Interleukin-7 Receptor alpha Subunit