Microwave-assisted Kochetkov amination followed by permanent charge derivatization: a facile strategy for glycomics.
We report a simple and rapid microwave-assisted method for the preparation of oligosaccharide-glycosylamines, followed by labelling with tris(2,4,6-trimethoxyphenyl)phosphonium acetic acid N-hydroxysuccinimide ester. The facile strategy introduced a permanent charge at the reducing end of the oligosaccharide. In combination of MALDI-MS, the detection limit for maltoheptaose was as low as 2 fmol μL(-1).
Liu
Xin
X
Department of Systems Biology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, China.
Zhang
Guisen
G
Chan
Kenneth
K
Li
Jianjun
J
D000586:Amination; D054794:Glycomics; D008872:Microwaves; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization