title: Significance of serum microRNAs in pre-diabetes and newly diagnosed type 2 diabetes: a clinical study.
abstract: To explore the clinical significance of seven diabetes-related serum microRNAs (miR-9, miR-29a, miR-30d, miR34a, miR-124a, miR146a and miR375) during the pathogenesis of type 2 diabetes (T2D), 56 subjects were recruited to this study: 18 cases of newly diagnosed T2D (n-T2D) patients, 19 cases of pre-diabetes individuals (impaired glucose tolerance [IGT] and/or impaired fasting glucose [IFG]) and 19 cases of T2D-susceptible individuals with normal glucose tolerance (s-NGT). Serum miRNAs were determined by real-time RT-PCR. Expression levels of single miRNAs and the expression signatures of miRNAs as a panel were analysed among the three groups. In n-T2D, all 7 miRNAs were significantly up-regulated compared with s-NGT and five were significantly up-regulated compared with pre-diabetes, while miRNA expression was not significantly different between s-NGT and pre-diabetes. By Canonical discriminant analysis, 70.6% of n-T2D subjects (12/17) were recognized by canonical discriminant function, while s-NGT and pre-diabetes subjects could not be discriminated from each other. Similar results were found in Hierarchical Clustering analysis based on the expression levels of all seven miRNAs. In different statistical analysis, miR-34a always showed the most significant differences. We conclude that the expression levels of seven diabetes-related miRNAs in serum were significantly elevated in n-T2D compared with pre-diabetes and/or s-NGT, and the latter two groups featured similar expression patterns of these miRNAs, suggesting that during the pathogenesis of T2D, the peripheral diabetes-related miRNAs have not changed significantly from s-NGT at pre-diabetic stage.
authors.affiliation: Department of Endocrinology, Provincial Hospital/Shandong University, 324 Jingwu Road, Jinan, People's Republic of China.
authors.forename: Lei
authors.initials: L
authors.lastname: Kong
authors.forename: Junjie
authors.initials: J
authors.lastname: Zhu
authors.forename: Wenxia
authors.initials: W
authors.lastname: Han
authors.forename: Xiuyun
authors.initials: X
authors.lastname: Jiang
authors.forename: Min
authors.initials: M
authors.lastname: Xu
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhao
authors.forename: Qiongzhu
authors.initials: Q
authors.lastname: Dong
authors.forename: Zengfen
authors.initials: Z
authors.lastname: Pang
authors.forename: Qingbo
authors.initials: Q
authors.lastname: Guan
authors.forename: Ling
authors.initials: L
authors.lastname: Gao
authors.forename: Jiajun
authors.initials: J
authors.lastname: Zhao
authors.forename: Lei
authors.initials: L
authors.lastname: Zhao
mesh_terms: D000328:Adult; D001786:Blood Glucose / Q000032:analysis; D003924:Diabetes Mellitus, Type 2 / Q000097:blood* / Q000175:diagnosis / Q000235:genetics* / Q000378:metabolism; D005260:Female; D005951:Glucose Tolerance Test; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood* / Q000235:genetics / Q000378:metabolism; D008875:Middle Aged; D011236:Prediabetic State / Q000097:blood* / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation
chemical_list: D001786:Blood Glucose; D035683:MicroRNAs