Protective effect of grape seed extract on gentamicin-induced acute kidney injury.
INTRODUCTION. This study was designed to determine the protective effect of red grape seed extract (RGSE) on gentamicin-induced nephrotoxicity in rats. MATERIALS AND METHODS. Thirty male Wistar rats were divided into 3 groups to receive RGSE, for 60 days followed by intraperitoneal injection of saline solution (as placebo) for 8 days (group 1); RGSE followed by gentamicin for 8 days (group 2); and gentamicin without pre-medication of RGSE (group 3). Oral RGSE, 40 mg/kg/d, and intraperitoneal injection of gentamicin, 100 mg/kg/d, were administered in these groups of rats. Blood and urine samples were collected on days 0 and 68 of the study. Then, the kidneys were removed for pathologic examination. RESULTS. On day 68, serum creatinine and blood urea nitrogen concentrations were highest in group 3, which was significantly higher than in group 1 (P = .001 and P = .004, respectively), while slightly higher than in group 2 (P = .30 and P = .50, respectively). Fractional excretion of sodium was not significantly different between the three groups. Histopathological evaluation showed that rats in group 3 had significantly higher degrees of severe acute tubular necrosis and interstitial mononuclear cell infiltration than the rats in groups 1 and 2 (P < .001). CONCLUSIONS. This animal study suggests that pretreatment with RGSE protects against gentamicin-induced acute kidney injury as evident on tissue histology. However, this was not accompanied with significant improvement in biochemical markers of kidney injury.
Safa
Javid
J
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Argani
Hassan
H
Bastani
Bahar
B
Nezami
Nariman
N
Rahimi Ardebili
Babak
B
Ghorbanihaghjo
Amir
A
Kalagheichi
Hassan
H
Amirfirouzi
Akbar
A
Mesgari
Mehran
M
Soleimany Rad
Jafar
J
D058186:Acute Kidney Injury / Q000139:chemically induced* / Q000473:pathology / Q000517:prevention & control*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000009:adverse effects*; D000975:Antioxidants / Q000627:therapeutic use*; D001806:Blood Urea Nitrogen; D003404:Creatinine / Q000097:blood; D005839:Gentamicins / Q000009:adverse effects*; D056604:Grape Seed Extract / Q000627:therapeutic use*; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D000900:Anti-Bacterial Agents; D000975:Antioxidants; D005839:Gentamicins; D056604:Grape Seed Extract; D003404:Creatinine