title: Chicken model of steroid-induced bone marrow adipogenesis using proteome analysis: a preliminary study.
abstract: BACKGROUND
  Steroid-induced adipogenesis increases fat-cell volume and pressure in bone marrow. This may be a contributing factor in some forms of osteonecrosis. In this observational study, we aimed to determine the protein expression relating to steroid-induced adipogenesis of femoral bone marrow with use of a chicken model. We compared the histologic features of the femoral marrow of eight methylprednisolone (MP)-treated chickens with those of three control chickens and assessed differential proteins with 2-dimensional gel electrophoresis and differential proteins were identified by MALDI-TOF MS.
  
  
  RESULTS
  One MP-induced chicken died of overdose anesthesia. Methylprednisolone-induced proliferation of adipose tissue and new bone formation were found on histologic examination. In our study, 13 proteins in the control and MP-induced groups were differently expressed and nine protein spots showed marked threefold downregulation after 19 weeks of MP treatment. These were serum amyloid P-component precursor, zinc finger protein 28, endothelial zinc finger protein 71, T-box transcription factor 3, cyclin-dependent kinase inhibitor 1, myosin 1D, dimethylaniline monooxygenase, and two uncharacterized proteins.
  
  
  CONCLUSIONS
  Proteomic profiling can be a useful dynamic approach for detecting protein expression in MP-induced adipogenesis of the femur in chickens.
authors.affiliation: Department of Radiology, School of Medicine, Taipei Medical University, Taipei 110, Taiwan, Republic of China. wp.chan@msa.hinet.net.
authors.forename: Sing Chung
authors.initials: SC
authors.lastname: Li
authors.forename: Ching Yu
authors.initials: CY
authors.lastname: Lin
authors.forename: Tzong Fu
authors.initials: TF
authors.lastname: Kuo
authors.forename: Yun Ho
authors.initials: YH
authors.lastname: Lin
authors.forename: Chia Chun
authors.initials: CC
authors.lastname: Chen
authors.forename: Way Neng
authors.initials: WN
authors.lastname: Lin
authors.forename: Wing P
authors.initials: WP
authors.lastname: Chan