A controlled release system of titanocene dichloride by electrospun fiber and its antitumor activity in vitro.
In order to improve both safety and efficacy of cancer chemotherapy of titanocene dichloride and overcome the shortcomings such as instability and short half-life in the human body, we report a controlled release system of titanocene dichloride by electrospun fiber and its in vitro antitumor activity against human lung tumor spca-1 cells. The system was developed by electrospinning. The release profiles of titanocene dichloride in PBS were researched by UV-Vis spectrophotometer. In vitro antitumor activities of the fibers were examined by MTT method. Titanocene dichloride was well incorporated in biodegradable poly(L-lactic acid) fibers. XRD results suggest that titanocene dichloride exists in the amorphous form in the fibers. The controlled release of titanocene dichloride can be gained for long time. MTT showed actual titanocene dichloride content 40, 80, 160 and 240 mg/L from the fibers mat, cell growth inhibition rates of 11.2%, 22.1%, 44.2% and 68.2% were achieved, respectively. The titanocene dichloride released has obvious inhibition effect against lung tumor cells. The system has an effect of controlled release of titanocene dichloride and may be used as an implantable anticancer drug in clinical applications in the future.
Chen
Ping
P
Department of Chemistry, Tongji University, Shanghai, PR China.
Wu
Qing-Sheng
QS
Ding
Ya-Ping
YP
Chu
Maoquan
M
Huang
Zheng-Ming
ZM
Hu
Wen
W
D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000494:pharmacology; D045744:Cell Line, Tumor; D003692:Delayed-Action Preparations; D016503:Drug Delivery Systems; D006207:Half-Life; D006801:Humans; D019344:Lactic Acid; D009942:Organometallic Compounds / Q000008:administration & dosage* / Q000494:pharmacology; D011091:Polyesters; D011108:Polymers
D000970:Antineoplastic Agents; D003692:Delayed-Action Preparations; D009942:Organometallic Compounds; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide); C020528:titanocene dichloride