title: Roles of L5-7 loop in the structure and chaperone function of SsHSP14.1.
abstract: The small heat shock protein SsHSP14.1 from the hyper-thermophilic archeaon, Sulfolobus solfataricus (S. solfataricus) was able to protect proteins from thermal aggregation and prevent enzymes from heat induced inactivation. According to the 3D (dimensional) structural model of SsHSP14.1 developed by us before, the region L5-7 (β5-β7, 68-82 residues) plays an important role for the oligomerization of SsHSP14.1 and its chaperone function. Here, to validate the findings, an in-depth investigation was conducted of both the wild type SsHSP14.1 and its deletion mutant DEL75-79. With E. coli proteins and bromelain as substrate, the deletion mutant DEL75-79 can protect them from thermo-aggregating as effective as the wild protein. Interestingly, unlike the wild protein, DEL75-79 was unable to prevent bromelain and EcoRI from thermo-inactivating. Results of size exclusion HPLC showed that the oligomerization state was changed in mutant protein. This was in accordance with the changed structure and lower hydrophobicity of DEL75-79. These outcomes proved that the L5-7 loop did play a role for the oligomerizing SsHSP14.1, and that the residues 75-79 were indispensable for its function of prevent enzymes from thermo-inactivating.
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, China.
authors.forename: Zhen-Zhen
authors.initials: ZZ
authors.lastname: Wen
authors.forename: Yong-Hua
authors.initials: YH
authors.lastname: Wang
authors.forename: Bo
authors.initials: B
authors.lastname: Yang
authors.forename: Ming-Quan
authors.initials: MQ
authors.lastname: Xie
authors.forename: Kuo-Chen
authors.initials: KC
authors.lastname: Chou
mesh_terms: D019843:Archaeal Proteins / Q000737:chemistry* / Q000378:metabolism*; D001709:Biotechnology; D006360:Heat-Shock Proteins / Q000737:chemistry* / Q000378:metabolism*; D057927:Hydrophobic and Hydrophilic Interactions; D008958:Models, Molecular; D055503:Protein Multimerization; D020836:Protein Structure, Quaternary; D048229:Sulfolobus solfataricus
chemical_list: D019843:Archaeal Proteins; D006360:Heat-Shock Proteins