title: Fertilization rate and number of embryos on day 2 after intrauterine and deep intrauterine insemination using frozen-thawed boar semen in multiparous sows.
abstract: The present study determines fertilization rate and number of embryos on Day 2 after intrauterine insemination (IUI) and deep intrauterine insemination (DIUI) using frozen-thawed (FT) boar semen in multiparous sows. Twelve crossbred Landrace × Yorkshire multiparous sows were included. The sows were inseminated at 24 h after oestrus detection and reinseminated every 12 h until ovulation took place. The inseminations were conducted using IUI with 2 × 10(9) FT sperm per dose (n = 6) and DIUI with 1 × 10(9) FT sperm per dose (n = 6). The sows were slaughtered at 45.1 ± 7.2 h after ovulation. Embryos and unfertilized oocytes were flushed from the oviducts. IUI yielded a better fertilization rate than DIUI (66.0% versus 31.0%, P < .001). The number of embryos was 13.5 ± 2.7 and 6.6 ± 3.2 embryos/sow in IUI and DIUI groups, respectively (P = .08). The proportion of sows having unilateral fertilization was higher in the DIUI (3/5) than the IUI group (1/6). In conclusion, IUI with at least 2 × 10(9) total number of FT boar spermatozoa is recommended.
authors.affiliation: Department of Obstetrics, Gynaecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand.
authors.forename: Kakanang
authors.initials: K
authors.lastname: Buranaamnuay
authors.forename: Yodchai
authors.initials: Y
authors.lastname: Panyaboriban
authors.forename: Padet
authors.initials: P
authors.lastname: Tummaruk
authors.forename: Mongkol
authors.initials: M
authors.lastname: Techakumphu