Turn-on fluorescence switch involving aggregation and elimination processes for β-lactamase-tag.
The targeted protein of interest is fused with genetically modified β-lactamase enzyme, which reacts with the probe in physiological conditions to break the aggregated interaction between the fluorophore and quencher. This alliance-separation technique is new for protein labeling and is probed in vitro and in live cell imaging studies.
Sadhu
Kalyan K
KK
Division of Advanced Science and Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
Mizukami
Shin
S
Watanabe
Shuji
S
Kikuchi
Kazuya
K
D002460:Cell Line; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D001618:beta-Lactamases / Q000737:chemistry*
D005456:Fluorescent Dyes; D001618:beta-Lactamases