title: Ensifer sojae sp. nov., isolated from root nodules of Glycine max grown in saline-alkaline soils.
abstract: Thirteen bacterial isolates from root nodules of soybean grown in saline-alkaline soils in the Chinese province of Hebei were identified as a unique group in the genus Ensifer based upon BOX-PCR patterns, sequencing analyses of 16S rRNA and housekeeping genes and DNA-DNA hybridization. Phenotypically, positive tests for acid production and negative results for reduction in litmus milk and sensitivity to 50 µg ampicillin ml(-1), as well as some other features, could differentiate the novel group from defined species of the Ensifer-Sinorhizobium group. The novel group had symbiotic gene sequences (nodC and nifH) that were identical or very similar to those of Ensifer (Sinorhizobium) fredii, and formed effective nodules with Glycine max (soybean), Vigna unguiculata and Glycine soja. Based upon the consensus of these analyses, a novel species, Ensifer sojae sp. nov., is proposed, with CCBAU 05684(T) ( = LMG 25493(T)  = HAMBI 3098(T)) as the type strain. The DNA G+C content of strain CCBAU 05684(T) was 60.9 mol% (T(m)).
authors.affiliation: State Key Laboratory of Agrobiotechnology and Department of Microbiology and Immunology, College of Biological Sciences, China Agricultural University, Beijing 100193, PR China.
authors.forename: Qin Qin
authors.initials: QQ
authors.lastname: Li
authors.affiliation: Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, 11340 México DF, Mexico.|State Key Laboratory of Agrobiotechnology and Department of Microbiology and Immunology, College of Biological Sciences, China Agricultural University, Beijing 100193, PR China.
authors.forename: En Tao
authors.initials: ET
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Agrobiotechnology and Department of Microbiology and Immunology, College of Biological Sciences, China Agricultural University, Beijing 100193, PR China.
authors.forename: Yue Li
authors.initials: YL
authors.lastname: Chang
authors.affiliation: State Key Laboratory of Agrobiotechnology and Department of Microbiology and Immunology, College of Biological Sciences, China Agricultural University, Beijing 100193, PR China.
authors.forename: Yun Zeng
authors.initials: YZ
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Agrobiotechnology and Department of Microbiology and Immunology, College of Biological Sciences, China Agricultural University, Beijing 100193, PR China.
authors.forename: Yan Ming
authors.initials: YM
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Agrobiotechnology and Department of Microbiology and Immunology, College of Biological Sciences, China Agricultural University, Beijing 100193, PR China.
authors.forename: Xin Hua
authors.initials: XH
authors.lastname: Sui
authors.affiliation: State Key Laboratory of Agrobiotechnology and Department of Microbiology and Immunology, College of Biological Sciences, China Agricultural University, Beijing 100193, PR China.
authors.forename: Wen Feng
authors.initials: WF
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Agrobiotechnology and Department of Microbiology and Immunology, College of Biological Sciences, China Agricultural University, Beijing 100193, PR China.
authors.forename: Wen Xin
authors.initials: WX
authors.lastname: Chen
mesh_terms: D000468:Alkalies / Q000032:analysis; D001426:Bacterial Proteins / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012230:Rhizobiaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D053204:Root Nodules, Plant / Q000382:microbiology; D012965:Sodium Chloride / Q000032:analysis; D012987:Soil / Q000032:analysis; D012988:Soil Microbiology; D013025:Glycine max / Q000254:growth & development / Q000382:microbiology*
chemical_list: D000468:Alkalies; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012987:Soil; D012965:Sodium Chloride