title: A reliable method to concentrate circulating DNA.
abstract: Concentration of circulating DNA probes is required to increase the amount of DNA involved in subsequent study (by polymerase chain reaction, sequencing, and microarray). This work was dedicated to the comparison of five different methods used for concentration of DNA circulating in blood. Precipitation of circulating DNA with acetone in the presence of triethylamine provides minimal DNA loss, high reproducibility, and at least three times higher DNA yield in comparison with the standard ethanol protocol.
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine, Novosibirsk 630090, Russia. olga.bryzgunova@niboch.nsc.ru
authors.forename: Olga
authors.initials: O
authors.lastname: Bryzgunova
authors.forename: Anna
authors.initials: A
authors.lastname: Bondar
authors.forename: Evgeniy
authors.initials: E
authors.lastname: Morozkin
authors.forename: Vladislav
authors.initials: V
authors.lastname: Mileyko
authors.forename: Valentin
authors.initials: V
authors.lastname: Vlassov
authors.forename: Pavel
authors.initials: P
authors.lastname: Laktionov
mesh_terms: D000096:Acetone / Q000737:chemistry; D004270:DNA, Circular / Q000097:blood / Q000737:chemistry / Q000302:isolation & purification*; D005021:Ethylamines / Q000737:chemistry; D005590:Fractional Precipitation / Q000379:methods*; D006801:Humans; D008297:Male; D011471:Prostatic Neoplasms / Q000097:blood / Q000235:genetics; D019346:Sodium Acetate / Q000737:chemistry
chemical_list: D004270:DNA, Circular; D005021:Ethylamines; D000096:Acetone; D019346:Sodium Acetate; C016162:triethylamine