[Study on 5-carboxyfluorescein N-succinimidyl ester in detecting the degree of cross-linked allogenic tissue].
This study was designed to creat a new method for detecting the degree of cross-linked allogenic tissue based on fluorescent technique. The thoracic aorta of New Zealand rabbits were divided randomly into four groups according to the concentration of Glutaraldehyde (GA), which were group A (control group-with no GA), group B (cross-linked with GA of 0.625%), group C (1.25%), and group D (2.5%). Each group was cross-linked with GA and reacted with 5-FAMSE, and then the fluorescence intensity was observed via fluorescence microscopy (analyzed with Image-Pro Plus 6.0, a professional image analysis software). The differences between groups in order of fluorescence intensity were found to be: group A > roup B > group C > group D (P < 0.01). Meanwhile, the tissue proteins extracted from aorta in each group were submitted to conventional polyacrylamide gel electrophoresis (PGE) after being cross-linked with GA; the result from this method was compared with that from the method of 5-FAMSE. In group A, the tissue proteins extracted from the aorta cross-linked with GA were obviously less than those not cross-linked with GA. However, this phenomenom was not clearly seen among the B, C and D groups. Nevertheless, 5-FAMSE can detect the degree of cross-linkage more conveniently and directly.
Zahng
Jun
J
Department of Cardiac Surgery, Qianfoshan Hospital, Shandong University, Ji'nan 250012, China.
Liu
Wenyi
W
Liu
Luqi
L
D000818:Animals; D001013:Aorta, Thoracic / Q000187:drug effects* / Q000637:transplantation; D001705:Bioprosthesis; D001807:Blood Vessel Prosthesis; D003432:Cross-Linking Reagents / Q000494:pharmacology*; D004952:Esters; D005260:Female; D005452:Fluoresceins; D005453:Fluorescence; D005976:Glutaral / Q000494:pharmacology*; D008297:Male; D008856:Microscopy, Fluorescence; D011817:Rabbits
D003432:Cross-Linking Reagents; D004952:Esters; D005452:Fluoresceins; C045132:4-carboxyfluorescein; D005976:Glutaral