Chiral separation of the β2-sympathomimetic fenoterol by HPLC and capillary zone electrophoresis for pharmacokinetic studies.
The development of methods for the separation of the enantiomers of fenoterol by chiral HPLC and capillary zone electrophoresis (CZE) is described. For the HPLC separation precolumn fluorescence derivatization with naphthyl isocyanate was applied. The resulting urea derivatives were resolved on a cellulose tris(3,5-dimethylphenylcarbamate)-coated silica gel column employing a column switching procedure. Detection was carried out fluorimetrically with a detection limit in the low ng/mL range. The method was adapted to the determination of fenoterol enantiomers in rat heart perfusates using liquid-liquid extraction. As an alternative a CE method was used for the direct separation of fenoterol enantiomers comparing different cyclodextrin derivatives as chiral selectors.
Ullrich
Thomas
T
Faculty of Biosciences, Institute of Biochemistry and Biotechnology, Martin Luther University Halle-Wittenberg, Kurt-Mothes-Str. 3, D-06120 Halle (Saale), Germany.
Wesenberg
Dirk
D
Bleuel
Corinna
C
Krauss
Gerd-Joachim
GJ
Schmid
Martin G
MG
Weiss
Michael
M
Gübitz
Gerald
G
D058666:Adrenergic beta-2 Receptor Agonists / Q000737:chemistry / Q000302:isolation & purification; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000379:methods*; D005280:Fenoterol / Q000737:chemistry* / Q000302:isolation & purification; D009206:Myocardium / Q000737:chemistry; D051381:Rats; D013237:Stereoisomerism; D013566:Sympathomimetics / Q000737:chemistry / Q000302:isolation & purification
D058666:Adrenergic beta-2 Receptor Agonists; D013566:Sympathomimetics; D005280:Fenoterol