A turn-on split-luciferase sensor for the direct detection of poly(ADP-ribose) as a marker for DNA repair and cell death.
Designed sensors comprising split-firefly luciferase conjugated to tandem poly(ADP-ribose) binding domains allow for the direct solution phase detection of picogram quantities of PAR and for monitoring temporal changes in poly(ADP-ribosyl)ation events in mammalian cells.
Furman
Jennifer L
JL
Department of Chemistry and Biochemistry, University of Arizona, 1306 East University Blvd, Tucson, AZ 85721, USA.
Mok
Pui-Wing
PW
Shen
Shengyi
S
Stains
Cliff I
CI
Ghosh
Indraneel
I
D014408:Biomarkers, Tumor / Q000032:analysis* / Q000378:metabolism; D015374:Biosensing Techniques / Q000379:methods*; D016923:Cell Death; D045744:Cell Line, Tumor; D004260:DNA Repair; D006367:HeLa Cells; D006801:Humans; D008156:Luciferases / Q000737:chemistry / Q000378:metabolism*; D008968:Molecular Conformation; D011064:Poly Adenosine Diphosphate Ribose / Q000032:analysis* / Q000378:metabolism
D014408:Biomarkers, Tumor; D011064:Poly Adenosine Diphosphate Ribose; D008156:Luciferases