title: Bioelectrocatalytic determination of nitrite ions based on polyaniline grafted nanodiamond.
abstract: Polyaniline chains were grafted onto nanodiamond (PANI-g-ND) through electrochemical polymerization of aniline in the presence of amine functionalized ND. A robust and effective composite film comprising PANI-g-ND/gold particles was subsequently prepared. Cytochrome c was successfully immobilized onto PANI-g-ND/Au film. Field emission scanning electron microscope (FESEM) image of PANI-g-ND/Au reveals the presence of fibrous PANI embedded into ND galleries with uniformly distributed Au clusters (∼1 μm). Direct electrochemistry and electrocatalysis of cyt c were investigated. PANI-g-ND/Au film showed an obvious direct electron transfer between cyt c and the underlying electrode. Cyclic voltammograms revealed that PANI-g-ND/Au/cyt c exhibited an excellent electrocatalysis towards the detection of nitrite ions. Differential pulse voltammetry of PANI-g-ND/Au/cyt c revealed a wide linear concentration range (0.5 μM-3 mM) for current responses, sensitivity (88.2 μA/mM) and low detection limit (0.16 μM) towards the electrochemical detection of nitrite ions.
authors.affiliation: Department of Chemistry Education, Kyungpook National University, Daegu, South Korea. algopal 99@yahoo.com
authors.forename: A I
authors.initials: AI
authors.lastname: Gopalan
authors.forename: K-P
authors.initials: KP
authors.lastname: Lee
authors.forename: S
authors.initials: S
authors.lastname: Komathi
mesh_terms: D000814:Aniline Compounds; D015374:Biosensing Techniques / Q000379:methods*; D045304:Cytochromes c; D055664:Electrochemical Techniques; D004579:Electron Transport; D004785:Environmental Pollutants / Q000032:analysis; D006046:Gold; D055767:Immobilized Proteins; D053768:Metal Nanoparticles / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D058612:Nanodiamonds; D009573:Nitrites / Q000032:analysis*; D010084:Oxidation-Reduction
chemical_list: D000814:Aniline Compounds; D004785:Environmental Pollutants; D055767:Immobilized Proteins; D058612:Nanodiamonds; D009573:Nitrites; C416807:polyaniline; D006046:Gold; D045304:Cytochromes c