title: Subcutaneous tissue reaction to castor oil bean and calcium hydroxide in rats.
abstract: Castor oil bean cement (COB) is a new material that has been used as an endodontic sealer, and is a candidate material for direct pulp capping.
  
  
  OBJECTIVE
  The purpose of this study was to evaluate the biocompatibility of a new formulation of COB compared to calcium hydroxide cement (CH) and a control group without any material, in the subcutaneous tissue of rats.
  
  
  METHODS
  The materials were prepared, packed into polyethylene tubes, and implanted in the rat dorsal subcutaneous tissue. Animals were sacrificed at the 7th and 50th days after implantation. A quantitative analysis of inflammatory cells was performed and data were subjected to ANOVA and Tukey's tests at 5% significance level.
  
  
  RESULTS
  Comparing the mean number of inflammatory cells between the two experimental groups (COB and CH) and the control group, statistically significant difference (p=0.0001) was observed at 7 and 50 days. There were no significant differences (p=0.111) between tissue reaction to CH (382 inflammatory cells) and COB (330 inflammatory cells) after 7 days. After 50 days, significantly more inflammatory cells (p=0.02) were observed in the CH group (404 inflammatory cells) than in the COB group (177 inflammatory cells).
  
  
  CONCLUSIONS
  These results demonstrate that the COB cement induces less inflammatory response within long periods.
authors.affiliation: Department of Bioscience and Oral Diagnosis, São José dos Campos Dental School, São Paulo State University, São José dos Campos, SP, Brazil.
authors.forename: Samira Esteves Afonso
authors.initials: SE
authors.lastname: Camargo
authors.forename: Sigmar de Mello
authors.initials: Sde M
authors.lastname: Rode
authors.forename: Renata Falchete
authors.initials: RF
authors.lastname: do Prado
authors.forename: Yasmin Rodarte
authors.initials: YR
authors.lastname: Carvalho
authors.forename: Carlos Henrique Ribeiro
authors.initials: CH
authors.lastname: Camargo
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D002126:Calcium Hydroxide / Q000494:pharmacology*; D002368:Castor Oil / Q000494:pharmacology*; D002452:Cell Count; D003238:Connective Tissue / Q000473:pathology; D004804:Eosinophils / Q000473:pathology; D015726:Giant Cells / Q000473:pathology; D007249:Inflammation / Q000473:pathology; D008214:Lymphocytes / Q000473:pathology; D008264:Macrophages / Q000473:pathology; D008297:Male; D008903:Minerals / Q000494:pharmacology; D018919:Neovascularization, Physiologic / Q000187:drug effects; D009504:Neutrophils / Q000473:pathology; D010950:Plasma Cells / Q000473:pathology; D057872:Pulp Capping and Pulpectomy Agents / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012116:Resins, Plant / Q000494:pharmacology; D016037:Single-Blind Method; D040521:Subcutaneous Tissue / Q000187:drug effects* / Q000473:pathology; D013997:Time Factors
chemical_list: D001672:Biocompatible Materials; D008903:Minerals; D057872:Pulp Capping and Pulpectomy Agents; D012116:Resins, Plant; C509865:poliquil; C025702:Dycal; D002368:Castor Oil; D002126:Calcium Hydroxide