MbtH-like proteins as integral components of bacterial nonribosomal peptide synthetases.
The biosynthesis of many natural products of clinical interest involves large, multidomain enzymes called nonribosomal peptide synthetases (NRPSs). In bacteria, many of the gene clusters coding for NRPSs also code for a member of the MbtH-like protein superfamily, which are small proteins of unknown function. Using MbtH-like proteins from three separate NRPS systems, we show that these proteins copurify with the NRPSs and influence amino acid activation. As a consequence, MbtH-like proteins are integral components of NRPSs.
Felnagle
Elizabeth A
EA
Department of Bacteriology, University of Wisconsin-Madison, Madison, WI 53706, USA.
Barkei
John J
JJ
Park
Hyunjun
H
Podevels
Angela M
AM
McMahon
Matthew D
MD
Drott
Donald W
DW
Thomas
Michael G
MG
D001419:Bacteria / Q000201:enzymology* / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002207:Capreomycin / Q000096:biosynthesis*; D005810:Multigene Family / Q000502:physiology*; D010453:Peptide Synthases / Q000235:genetics / Q000378:metabolism*; D014756:Viomycin / Q000096:biosynthesis*
D001426:Bacterial Proteins; D002207:Capreomycin; D010453:Peptide Synthases; D014756:Viomycin