The treatment of propofol induced the TGF-β1 expression in human endothelial cells to suppress endocytosis activities of monocytes.
Propofol anesthesia and sedation are known to downregulate the functions of many hematopoietic cells, such as macrophages and neutrophils, in vivo. However, the effects of propofol on secretion of the regulatory cytokine transforming growth factor β1 (TGF-β1) in vivo are unknown. In this study, the effects of propofol on TGF-β1 expression in human peripheral blood mononuclear cells, umbilical vein endothelial cells (HUVECs), lymphocytes (Jurkat) and monocytes (THP-1) were tested. Moreover, these sera were also tested for regulatory activity on monocyte endocytosis with or without treatment with the TGF-β1 pathway inhibitor SB431542. Propofol raised levels of both total and activated TGF-β1 in propofol-treated patient sera after surgical operations. Furthermore, propofol induced secretion of latent TGF-β1 in HUVEC cells and enhanced TGF-β1 activation in THP-1 and Jurkat cells in vitro. Additionally, sera from propofol-treated patients suppressed monocyte endocytosis ex vivo, an effect that was abrogated by the TGF-β1 pathway inhibitor SB431542.
Li
Chi-Han
CH
Department of Biological Science and Technology, National Chiao Tung University, 75 Bo-Ai Street, Hsinchu, Taiwan, ROC.
Lee
Ru-Ping
RP
Lin
Yu-Ling
YL
Lin
Chen-Si
CS
Hsu
Bang-Gee
BG
Tseng
Feng-Jen
FJ
Chen
Yu-Cheng
YC
Liao
Kuang-Wen
KW
Yang
Fwu-Lin
FL
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D018686:Anesthetics, Intravenous / Q000494:pharmacology*; D002460:Cell Line; D004705:Endocytosis / Q000187:drug effects*; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000166:cytology*; D015742:Propofol / Q000494:pharmacology*; D053773:Transforming Growth Factor beta1 / Q000378:metabolism*
D018686:Anesthetics, Intravenous; D053773:Transforming Growth Factor beta1; D015742:Propofol