title: Highly efficient chemoenzymatic synthesis of β1-3-linked galactosides.
abstract: A novel D-galactosyl-β1-3-N-acetyl-D-hexosamine phosphorylase cloned from Bifidobacterium infantis (BiGalHexNAcP) was used with a recombinant E. coli K-12 galactokinase (GalK) for efficient one-pot two-enzyme synthesis of T-antigens, galacto-N-biose (Galβ1-3GalNAc), lacto-N-biose (Galβ1-3GlcNAc), and their derivatives.
authors.affiliation: Department of Chemistry, University of California, One Shields Avenue, Davis, California 95616, USA.
authors.forename: Hai
authors.initials: H
authors.lastname: Yu
authors.forename: Vireak
authors.initials: V
authors.lastname: Thon
authors.forename: Kam
authors.initials: K
authors.lastname: Lau
authors.forename: Li
authors.initials: L
authors.lastname: Cai
authors.forename: Yi
authors.initials: Y
authors.lastname: Chen
authors.forename: Shengmao
authors.initials: S
authors.lastname: Mu
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Li
authors.forename: Peng George
authors.initials: PG
authors.lastname: Wang
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
mesh_terms: D001644:Bifidobacterium / Q000201:enzymology*; D004926:Escherichia coli / Q000201:enzymology*; D005697:Galactosides / Q000737:chemistry / Q000378:metabolism*; D005700:Galactosyltransferases / Q000378:metabolism*; D007218:Industrial Microbiology; D015394:Molecular Structure
chemical_list: D005697:Galactosides; D005700:Galactosyltransferases; C118176:beta-1,3-galactosyl-N-acetylhexosamine phosphorylase