Constituents with α-glucosidase and advanced glycation end-product formation inhibitory activities from Salvia miltiorrhiza Bge.
The 75% ethanol extract from roots of Salvia miltiorrhiza Bge. (Dan shen) afforded two new compounds, 3-hydroxy-2-(2'-formyloxy-1'-methylethyl)-8-methyl-1,4-phenanthrenedione (1), (8'R)-isosalvianolic acid C methyl ester (2), and 14 known compounds. Their structures were established on the basis of spectral analysis. The ability of the compounds to inhibit α-glucosidase activity and formation of advanced glycation end-products (AGEs) was evaluated. All compounds displayed various degrees of inhibitory effects against α-glucosidase; moreover, compounds 2, 6, 11, 14, and 16 exhibited much more potent inhibition against AGEs than the positive control (aminoguanidine, AG, IC(50) 0.11 μM). This is the first time that compounds from this plant have been reported to have inhibitory activity against α-glucosidase.
Ma
Hai-Ying
HY
Pharmacy Department, The Fourth Affiliated Hospital of China Medical University, No. 4, Chongshan Eastern Road, Yuhong District, Shenyang 110032, China. cmu4h-mhy@126.com
Gao
Hui-Yuan
HY
Sun
Lu
L
Huang
Jian
J
Xu
Xiao-Min
XM
Wu
Li-Jun
LJ
D002851:Chromatography, High Pressure Liquid; D004789:Enzyme Activation / Q000187:drug effects; D017127:Glycation End Products, Advanced / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry; D027561:Salvia miltiorrhiza / Q000737:chemistry*; D000520:alpha-Glucosidases / Q000378:metabolism*
D017127:Glycation End Products, Advanced; D010936:Plant Extracts; D000520:alpha-Glucosidases