title: Transgenic rice plants expressing a fused protein of Cry1Ab/Vip3H has resistance to rice stem borers under laboratory and field conditions.
abstract: Six transgenic rice, Oryza sativa L., lines (G6H1, G6H2, G6H3, G6H4, G6H5, and G6H6) expressing a fused Cry1Ab/Vip3H protein, were evaluated for resistance against the Asiatic rice borer, Chilo suppressalis (Walker) (Lepidoptera: Crambidae), and the stem borer Sesamia inferens (Walker) (Lepidoptera: Noctuidae) in the laboratory and field. The bioassay results indicated that the mortality of Asiatic rice borer and S. inferens neonate larvae on six transgenic lines from seedling to filling stage was up to 100% at 168 h after infestation. The cumulative feeding area by Asiatic rice borer neonate larvae on all transgenic lines was significantly reduced compared with the untransformed parental 'Xiushui 110' rice. A 2-yr field evaluation showed that damage during the vegetative stage (deadheart) or during the reproductive stage (whitehead) caused by Asiatic rice borer and S. inferens for transgenic lines was much lower than the control. For three lines (G6H1, G6H2, and G6H6), no damage was found during the entire growing period. Estimation of fused Cry1Ab/Vip3H protein concentrations using PathoScreen kit for Bt-Cry1Ab/1Ac protein indicated that the expression levels of Cry1Ab protein both in main stems (within the average range of 0.006-0.073% of total soluble protein) and their flag leaves (within the average range of 0.001-0.038% of total soluble protein) were significantly different among six transgenic lines at different developmental stages. Both laboratory and field researches suggested that the transgenic rice lines have considerable potential for protecting rice from attack by both stem borers.
authors.affiliation: State Key Laboratory of Rice Biology & Ministry of Agriculture, Institute of Insect Sciences, Zhejiang University, Hangzhou 310029, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Chen
authors.forename: Jun-Ce
authors.initials: JC
authors.lastname: Tian
authors.forename: Zhi-Chen
authors.initials: ZC
authors.lastname: Shen
authors.forename: Yu-Fa
authors.initials: YF
authors.lastname: Peng
authors.forename: Cui
authors.initials: C
authors.lastname: Hu
authors.forename: Yu-Yuan
authors.initials: YY
authors.lastname: Guo
authors.forename: Gong-Yin
authors.initials: GY
authors.lastname: Ye
mesh_terms: D000818:Animals; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000235:genetics* / Q000494:pharmacology*; D004731:Endotoxins / Q000235:genetics* / Q000494:pharmacology*; D006460:Hemolysin Proteins / Q000235:genetics* / Q000494:pharmacology*; D009036:Moths / Q000187:drug effects*; D012275:Oryza / Q000235:genetics* / Q000378:metabolism* / Q000469:parasitology; D030821:Plants, Genetically Modified
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis