The application of heated detergent dewaxing and rehydration to immunohistochemistry.
Hot commercial dishwashing detergent has been used to deparaffinize and hydrate formalin fixed, paraffin embedded sections for immunohistochemistry. Fifty-five antibodies, used routinely for diagnosis, were used to compare hot detergent dewaxing with the proprietary hydrocarbon-based dewaxing reagent supplied with the Bond Max immunohistochemistry system®. A 2% concentration of commercial dishwashing detergent in distilled water was heated to 90° C and paraffin sections were treated twice for 1 min each. Nearly all antibodies gave equivalent results except CD10 and CD57 (hydrocarbon-based dewaxing better) and CD45 and alpha fetoprotein (detergent dewaxing better); the differences, however, were minimal. There also was a significant cost saving using detergent dewaxing.
Henwood
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Histopathology Department, The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, Australia. anthonyh@chw.edu.au
D000906:Antibodies / Q000032:analysis*; D003902:Detergents / Q000737:chemistry*; D005404:Fixatives; D005557:Formaldehyde; D006361:Heating; D006652:Histological Techniques / Q000379:methods; D007150:Immunohistochemistry / Q000379:methods*; D016612:Paraffin Embedding; D011786:Quality Control; D015203:Reproducibility of Results; D013194:Staining and Labeling / Q000379:methods*
D000906:Antibodies; D003902:Detergents; D005404:Fixatives; D005557:Formaldehyde