title: Cell behavior on extracellular matrix mimic materials based on mussel adhesive protein fused with functional peptides.
abstract: Adhesion of cells to surfaces is a basic and important requirement in cell culture and tissue engineering. Here, we designed artificial extracellular matrix (ECM) mimics for efficient cellular attachment, based on mussel adhesive protein (MAP) fusion with biofunctional peptides originating from ECM materials, including fibronectin, laminin, and collagen. Cellular behaviors, including attachment, proliferation, spreading, viability, and differentiation, were investigated with the artificial ECM material-coated surfaces, using three mammalian cell lines (pre-osteoblast, chondrocyte, and pre-adipocyte). All cell lines examined displayed superior attachment, proliferation, spreading, and survival properties on the MAP-based ECM mimics, compared to other commercially available cell adhesion materials, such as poly-L-lysine and the naturally extracted MAP mixture. Additionally, the degree of differentiation of pre-osteoblast cells on MAP-based ECM mimics was increased. These results collectively demonstrate that the artificial ECM mimics developed in the present work are effective cell adhesion materials. Moreover, we expect that the MAP peptide fusion approach can be extended to other functional tissue-specific motifs.
authors.affiliation: Department of Chemical Engineering, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea.
authors.forename: Bong-Hyuk
authors.initials: BH
authors.lastname: Choi
authors.forename: Yoo Seong
authors.initials: YS
authors.lastname: Choi
authors.forename: Dong Gyun
authors.initials: DG
authors.lastname: Kang
authors.forename: Bum Jin
authors.initials: BJ
authors.lastname: Kim
authors.forename: Young Hoon
authors.initials: YH
authors.lastname: Song
authors.forename: Hyung Joon
authors.initials: HJ
authors.lastname: Cha
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D040761:Biomimetic Materials / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D005109:Extracellular Matrix / Q000737:chemistry*; D051379:Mice; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects; D010455:Peptides / Q000302:isolation & purification / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism*; D013499:Surface Properties / Q000187:drug effects
chemical_list: D010455:Peptides; D011506:Proteins; D011993:Recombinant Fusion Proteins; C037403:adhesive protein, mussel