title: Digital subtraction radiographic analysis of the combination of bioabsorbable membrane and bovine morphogenetic protein pool in human periodontal infrabony defects.
abstract: OBJECTIVE
  This study assessed the bone density gain and its relationship with the periodontal clinical parameters in a case series of a regenerative therapy procedure.
  
  
  METHODS
  Using a split-mouth study design, 10 pairs of infrabony defects from 15 patients were treated with a pool of bovine bone morphogenetic proteins associated with collagen membrane (test sites) or collagen membrane only (control sites). The periodontal healing was clinically and radiographically monitored for six months. Standardized pre-surgical and 6-month postoperative radiographs were digitized for digital subtraction analysis, which showed relative bone density gain in both groups of 0.034 ± 0.423 and 0.105 ± 0.423 in the test and control group, respectively (p>0.05).
  
  
  RESULTS
  As regards the area size of bone density change, the influence of the therapy was detected in 2.5 mm² in the test group and 2 mm² in the control group (p>0.05). Additionally, no correlation was observed between the favorable clinical results and the bone density gain measured by digital subtraction radiography (p>0.05).
  
  
  CONCLUSIONS
  The findings of this study suggest that the clinical benefit of the regenerative therapy observed did not come with significant bone density gains. Long-term evaluation may lead to a different conclusions.
authors.affiliation: Periodontics Division, University of Brasilia, Brasília, DF, Brazil. mmgcarmo@gmail.com
authors.forename: Maria do Carmo Machado
authors.initials: Mdo C
authors.lastname: Guimarães
authors.forename: Euloir
authors.initials: E
authors.lastname: Passanezi
authors.forename: Adriana Campos Passanezi
authors.initials: AC
authors.lastname: Sant'Ana
authors.forename: Sebastião Luiz Aguiar
authors.initials: SL
authors.lastname: Grechi
authors.forename: Mario
authors.initials: M
authors.lastname: Taba Junior
mesh_terms: D020341:Absorbable Implants; D000328:Adult; D016301:Alveolar Bone Loss / Q000000981:diagnostic imaging / Q000601:surgery*; D000818:Animals; D015519:Bone Density / Q000187:drug effects; D001857:Bone Matrix / Q000637:transplantation; D019485:Bone Morphogenetic Proteins / Q000627:therapeutic use*; D018786:Bone Substitutes / Q000627:therapeutic use; D002417:Cattle; D003094:Collagen; D017886:Durapatite / Q000627:therapeutic use; D005500:Follow-Up Studies; D016557:Guided Tissue Regeneration, Periodontal / Q000295:instrumentation / Q000379:methods; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008567:Membranes, Artificial; D008875:Middle Aged; D017622:Periodontal Attachment Loss / Q000601:surgery; D010514:Periodontal Pocket / Q000601:surgery; D016300:Radiography, Bitewing; D013382:Subtraction Technique; D013524:Surgical Flaps; D016896:Treatment Outcome
chemical_list: D019485:Bone Morphogenetic Proteins; D018786:Bone Substitutes; D008567:Membranes, Artificial; D003094:Collagen; D017886:Durapatite