[Sequence analysis of the matrix protein and fusion protein genes of a field peste des petits ruminants virus strain from Tibet, China].
The nucleotide sequences of M and F genes from a field strain of peste des petits ruminants virus (PPRV) ("China/Tib/Gej/07-30") was firstly determined. The M gene was 1 483 nucleotides in length with a single open reading frame (ORF), encoding a protein of 335 amino acids. The F gene was 2411 nucleotides in length, encoding a protein of 546 amino acids. The resulting nucleotide sequence and the deduced amino acid sequences were compared with the homologous regions of other PPRV isolates. The nucleotide sequences of M and F genes of the "China/Tib/Gej/07-30" was 92.4%-97.7% and 85.5%-96.1% identical to other PPRV isolates, respectively, while a homology of 97.0%-98.2% and 94.3%-98.2% could be observed at the amino acids level respectively. Several sequence motifs in the M and F genes had been identified on the basis of conservation in the PPRVs and the morbilliviruses. The 3' untranslated region of M gene was 443 nucleotides in length with 82.4%-93.5% identical to other PPRV isolates. The 5' untranslated region of F gene was 634 nucleotides in length with 76.2%-91.7% identical to other PPRV isolates.
Bao
Jing-Yue
JY
National Diagnostic Center for Exotic Animal Diseases, China Animal Health and Epidemiology Center, Qingdao 266032, China.
Zhao
Wen-Ji
WJ
Wang
Zhi-Liang
ZL
Li
Lin
L
Wu
Guo-Zhen
GZ
Wu
Xiao-Dong
XD
Liu
Chun-Ju
CJ
Wang
Jun-Wei
JW
Liu
Yu-Tian
YT
Li
Jin-Ming
JM
Wang
Ying-Li
YL
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D008969:Molecular Sequence Data; D029021:Peste-des-Petits-Ruminants / Q000662:veterinary* / Q000821:virology; D018107:Peste-des-petits-ruminants virus / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid; D012756:Sheep; D012757:Sheep Diseases / Q000821:virology*; D018609:Tibet; D014760:Viral Fusion Proteins / Q000737:chemistry / Q000235:genetics*; D014763:Viral Matrix Proteins / Q000737:chemistry / Q000235:genetics*
D014760:Viral Fusion Proteins; D014763:Viral Matrix Proteins