Molecular cloning and expression analysis of feline α1-microglobulin.
Full-length cDNA that encodes feline α₁-microglobulin (Feα₁m)-bikunin was obtained from a feline liver and cloned using an oligo-capping method. The Feα₁m-bikunin cDNA was found to contain 1284 nucleotides, and Feα₁m was found to include an open reading frame encoding a polypeptide of 201 amino acids. The deduced amino acid sequence of Feα₁m showed varying amino acid identity when compared with the published sequences of the related α₁-m of other species, ranging from 71.1 to 82.1%. Feα₁m mRNA expression was confirmed by reverse transcription polymerase chain reaction (RT-PCR) and real-time PCR analysis in the cerebrum, cerebellum, lung, heart, liver, spleen, pancreas, kidney, adrenal gland, and testicle. The highest Feα₁m mRNA level was found in the liver.
Nakata
Junya
J
Department of Small Animal Internal Medicine, School of Veterinary Medicine and Animal Science, Kitasato University, Higashi, Japan.
Nakahari
Aiko
A
Kato
Yohei
Y
Yamachi
Nanako
N
Sato
Nami
N
Yonezawa
Tomohiro
T
Takahashi
Chika
C
Chikazawa
Seishiro
S
Hori
Yasutomo
Y
Kanai
Kazutaka
K
Ito
Naoyuki
N
Higuchi
Seiichi
S
Hoshi
Fumio
F
D000510:Alpha-Globulins / Q000096:biosynthesis / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002415:Cats / Q000235:genetics; D003001:Cloning, Molecular; D008099:Liver / Q000378:metabolism; D016366:Open Reading Frames / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment
D000510:Alpha-Globulins; D012333:RNA, Messenger; C016001:alpha-1-microglobulin