Ibuprofen augments pro-inflammatory cytokine release in a mouse model of Vibrio vulnificus infection.
We evaluated the effects of ibuprofen on cytokine production and mortality in a mouse model of septic shock induced by Vibrio vulnificus, strain Chi Mei Vv05191. Ibuprofen (50 mg/kg) or saline (control) was given to female BALB/cByJ mice for three consecutive days before exposure to the pathogen. For cytokine production, serum and peritoneal fluid were assayed for IL-1β, IL-6, TNF-α, and MIP-2 by ELISA at 3, 6, and 9 hr after intraperitoneal infection of the organism. At 6 hr after infection, serum and peritoneal fluid levels of IL-6, TNF-α, and MIP-2 were significantly higher in the ibuprofen group. For mortality determination, 73 mice (37 ibuprofen, 36 control) were injected intramuscularly with V. vulnificus. Kaplan-Meier survival curves were analyzed. Survival was significantly decreased by ibuprofen only for the lowest inoculum (25 CFU) of V. vulnificus. Administration of ibuprofen before infection may augment the pathogenesis of V. vulnificus by stimulating cytokine production.
Lee
Yuarn-Jang
YJ
Section of Infectious Diseases, Department of Internal Medicine, Taipei Medical University Hospital, 252 Wu Hsing Street, Taipei, Taiwan.
Chuang
Yin-Ching
YC
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D016207:Cytokines / Q000096:biosynthesis*; D004195:Disease Models, Animal; D005260:Female; D007052:Ibuprofen / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D014735:Vibrio Infections / Q000276:immunology* / Q000401:mortality; D041261:Vibrio vulnificus
D000894:Anti-Inflammatory Agents, Non-Steroidal; D016207:Cytokines; D007052:Ibuprofen