A yeast bioassay for androgenic and anti-androgenic compounds based on the NH2- and COOH-terminal interaction of androgen receptor.
Androgenic compounds induce an interaction between the NH(2)- and COOH-terminal regions (N-C interaction) of androgen receptor (AR). We describe a rapid yeast bioassay for androgenic and anti-androgenic compounds based on androgen-dependent β-catenin-enhanced N-C interaction. The bioassay was also effective at detecting compounds that inhibit the N-C interaction in ways that do not involve binding to the ligand-binding domain.
Ogawa
Masahiro
M
Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Osaka, Japan.
Yamaji
Ryoichi
R
Mitani
Takakazu
T
Murata
Yohei
Y
Nakao
Motoyuki
M
Harada
Naoki
N
Nakano
Yoshihisa
Y
Inui
Hiroshi
H
D000726:Androgen Antagonists / Q000032:analysis*; D000728:Androgens / Q000032:analysis*; D000818:Animals; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D006801:Humans; D011944:Receptors, Androgen / Q000737:chemistry* / Q000378:metabolism; D015003:Yeasts; D051176:beta Catenin
D000726:Androgen Antagonists; D000728:Androgens; D011944:Receptors, Androgen; D051176:beta Catenin