Intrinsic disorder and oligomerization of the hepatitis delta virus antigen.
The 195 amino acid basic protein (δAg) of hepatitis delta virus (HDV) is essential for replication of the HDV RNA genome. Numerous properties have been mapped to full-length δAg and attempts made to link these to secondary, tertiary and quaternary structures. Here, for the full-size δAg, extensive intrinsic disorder was predicted using PONDR-FIT, a meta-predictor of intrinsic disorder, and evidenced by circular dichroism measurements. Most δAg amino acids are in disordered configurations with no more than 30% adopting an α-helical structure. In addition, dynamic light scattering studies indicated that purified δAg assembled into structures of as large as dodecamers. Cross-linking followed by denaturing polyacrylamide gel electrophoresis revealed hexamers to octamers for this purified δAg and at least this size for δAg found in virus-like particles. Oligomers of purified δAg were resistant to elevated NaCl and urea concentrations, and bound without specificity to RNA and single- and double-stranded DNAs.
Alves
Carolina
C
Fox Chase Cancer Center, Philadelphia, PA 19111, USA. carolinamantero@gmail.com
Cheng
Hong
H
Roder
Heinrich
H
Taylor
John
J
D000595:Amino Acid Sequence; D000818:Animals; D002942:Circular Dichroism; D004279:DNA, Viral / Q000235:genetics / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D003698:Hepatitis Delta Virus / Q000737:chemistry / Q000378:metabolism; D035923:Hepatitis delta Antigens / Q000737:chemistry* / Q000378:metabolism*; D008969:Molecular Sequence Data; D055503:Protein Multimerization; D020836:Protein Structure, Quaternary; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism; D012984:Software
D004279:DNA, Viral; D035923:Hepatitis delta Antigens; D012367:RNA, Viral