Quantification of proteins by label-free LC-MS/MS.
Quantitative proteomic profiling is becoming a widely used approach in systems biology and biomarker discovery. There is a growing realization that quantitative studies require high numbers of non-pooled samples for increased statistical power. We present a descriptive protocol for label-free quantitation of proteins by LC-MS/MS that enables to obtain both quantitative and qualitative information in one study without the need to pool samples or label them.
Levin
Yishai
Y
Institute of Biotechnology, University of Cambridge, Cambridge, UK.
Bahn
Sabine
S
D000818:Animals; D002417:Cattle; D002853:Chromatography, Liquid / Q000379:methods*; D030562:Databases, Protein; D036103:Nanotechnology; D011506:Proteins / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D015203:Reproducibility of Results; D013194:Staining and Labeling; D013223:Statistics as Topic; D053719:Tandem Mass Spectrometry / Q000379:methods*
D011506:Proteins