title: Development of digestive enzymes in larvae of Mayan cichlid Cichlasoma urophthalmus.
abstract: The development of digestive enzymes during the early ontogeny of the Mayan cichlid (Cichlasoma urophthalmus) was studied using biochemical and electrophoretic techniques. From yolk absorption (6 days after hatching: dah), larvae were fed Artemia nauplii until 15 dah, afterward they were fed with commercial microparticulated trout food (45% protein and 16% lipids) from 16 to 60 dah. Several samples were collected including yolk-sac larvae (considered as day 1 after hatching) and specimens up to 60 dah. Most digestive enzymes were present from yolk absorption (5-6 dah), except for the specific acid proteases activity (pepsin-like), which increase rapidly from 8 dah up to 20 dah. Three alkaline proteases isoforms (24.0, 24.8, 84.5 kDa) were detected at 8 dah using SDS-PAGE zymogram, corresponding to trypsin, chymotrypsin and probably leucine aminopeptidase enzymes, and only one isoform was detected (relative electromobility, Rf = 0.54) for acid proteases (pepsin-like) from 3 dah onwards using PAGE zymogram. We concluded that C. urophthamus is a precocious fish with a great capacity to digest all kinds of food items, including artificial diets provided from 13 dah.
authors.affiliation: Laboratorio de Acuicultura Tropical-DACBIOL, Universidad Juárez Autónoma de Tabasco, Carretera Villahermosa Cárdenas km 0.5, Villahermosa, Tabasco, México.
authors.forename: G
authors.initials: G
authors.lastname: López-Ramírez
authors.forename: C A
authors.initials: CA
authors.lastname: Cuenca-Soria
authors.forename: C A
authors.initials: CA
authors.lastname: Alvarez-González
authors.forename: D
authors.initials: D
authors.lastname: Tovar-Ramírez
authors.forename: J L
authors.initials: JL
authors.lastname: Ortiz-Galindo
authors.forename: N
authors.initials: N
authors.lastname: Perales-García
authors.forename: G
authors.initials: G
authors.lastname: Márquez-Couturier
authors.forename: L
authors.initials: L
authors.lastname: Arias-Rodríguez
authors.forename: J R
authors.initials: JR
authors.lastname: Indy
authors.forename: W M
authors.initials: WM
authors.lastname: Contreras-Sánchez
authors.forename: E
authors.initials: E
authors.lastname: Gisbert
authors.forename: F J
authors.initials: FJ
authors.lastname: Moyano
mesh_terms: D000818:Animals; D023681:Cichlids / Q000254:growth & development*; D004798:Enzymes / Q000378:metabolism*; D007814:Larva / Q000201:enzymology / Q000254:growth & development; D013997:Time Factors
chemical_list: D004798:Enzymes