Chemoselective attachment of small molecule effector functionality to human adenoviruses facilitates gene delivery to cancer cells.
We demonstrate here a novel two-step "click" labeling process in which adenoviral particles are first metabolically labeled during production with unnatural azido sugars. Subsequent chemoselective modification allows access to viruses decorated with a broad array of effector functionality. Adenoviruses modified with folate, a known cancer-targeting motif, demonstrated a marked increase in gene delivery to a murine cancer cell line.
Banerjee
Partha Sarathi
PS
Department of Chemistry, State University of New York Stony Brook, New York 11790, USA.
Ostapchuk
Philomena
P
Hearing
Patrick
P
Carrico
Isaac
I
D000256:Adenoviridae / Q000737:chemistry / Q000235:genetics*; D000818:Animals; D001386:Azides / Q000737:chemistry*; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D002470:Cell Survival; D005260:Female; D018014:Gene Transfer Techniques; D006801:Humans; D051379:Mice; D015394:Molecular Structure
D001386:Azides