title: Strategies to improve pregnancy per insemination using sex-sorted semen in dairy heifers detected in estrus.
abstract: The objective was to improve pregnancy per artificial insemination (P/AI; 35-42 d after AI) in virgin Jersey heifers bred by AI of sex-sorted semen after being detected in estrus. Giving 100 μg of GnRH at first detection of estrus, with AI 12 h later, did not affect P/AI in Experiment I [GnRH = 47.2% (100/212) vs. No GnRH = 51.7% (104/201); P = 0.38] or Experiment II [GnRH = 53.1% (137/258) vs. No GnRH = 48.6% (122/251); P = 0.43]. In these two experiments, estrus detection was done with tail-head chalk or a HeatWatch(®) system, respectively. In Experiment III, a single insemination dose (2.1 × 10⁶ sperm) 12 h after estrus detection (n = 193), a double dose at 12 h (n = 193), or a double dose involving insemination 12 and 24 h after estrus detection (n = 190) did not affect P/AI (87/193 = 45.1%, 85/193 = 44.0%, and 94/190 = 49.5%, respectively; P = 0.51). However, P/AI was influenced by the number of AI service (First, 115/208 = 55.3%(a); Second, 94/204 = 46.1%(a); and Third, 57/165 = 34.8%(b); P = 0.004). In Experiment IV, the P/AI of heifers inseminated from 12 to 16 h after the onset of estrus (40/106 = 37.7%) was less (P = 0.03) than those inseminated from 16.1 to 20 h (85/164 = 51.8%), and 20.1 to 24 h (130/234 = 55.6%). However, the P/AI for heifers inseminated from 24.1 to 30 h (61/134 = 45.5%) did not differ from that of any other interval. In conclusion, in Jersey heifers inseminated with sex-sorted semen, P/AI was not significantly affected by giving GnRH at detection of estrus or a double insemination dose, but it was higher with AI 16.1 to 24 h vs. 12 to 16 h after the onset of estrus.
authors.affiliation: Departamento de Reprodução Animal, FMVZ-USP, CEP 05.508-000, São Paulo, SP, Brasil.
authors.forename: M F
authors.initials: MF
authors.lastname: Sá Filho
authors.forename: H
authors.initials: H
authors.lastname: Ayres
authors.forename: R M
authors.initials: RM
authors.lastname: Ferreira
authors.forename: M
authors.initials: M
authors.lastname: Nichi
authors.forename: M
authors.initials: M
authors.lastname: Fosado
authors.forename: E P
authors.initials: EP
authors.lastname: Campos Filho
authors.forename: P S
authors.initials: PS
authors.lastname: Baruselli
mesh_terms: D000818:Animals; D002417:Cattle; D004971:Estrus; D004972:Estrus Detection / Q000379:methods; D005260:Female; D005434:Flow Cytometry; D007987:Gonadotropin-Releasing Hormone / Q000494:pharmacology; D007315:Insemination, Artificial / Q000379:methods / Q000662:veterinary*; D011247:Pregnancy; D018873:Pregnancy Rate; D012661:Semen / Q000166:cytology*; D012732:Sex Determination Analysis; D013997:Time Factors
chemical_list: D007987:Gonadotropin-Releasing Hormone