High-throughput screening of improved protease inhibitors using a yeast cell surface display system and a yeast cell chip.
Protease-targeted inhibitors have been promising pharmaceuticals. Here, we combined a yeast cell surface display system with a yeast cell chip for the high-throughput screening of protease inhibitors, and succeeded in improving the activity of a protease inhibitor.
Aoki
Wataru
W
Japan Society for the Promotion of Science, Sakyo-ku, Kyoto 606-8502, Japan.
Yoshino
Yuichi
Y
Morisaka
Hironobu
H
Tsunetomo
Keiji
K
Koyo
Hirotaka
H
Kamiya
Shinji
S
Kawata
Noriyuki
N
Kuroda
Kouichi
K
Ueda
Mitsuyoshi
M
D004353:Drug Evaluation, Preclinical; D057166:High-Throughput Screening Assays / Q000379:methods*; D007147:Immunoglobulin Light Chains / Q000737:chemistry; D008666:Metalloendopeptidases / Q000037:antagonists & inhibitors; D011480:Protease Inhibitors / Q000494:pharmacology*; D011993:Recombinant Fusion Proteins / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000378:metabolism
D007147:Immunoglobulin Light Chains; D011480:Protease Inhibitors; D011993:Recombinant Fusion Proteins; D008666:Metalloendopeptidases; C062316:neurolysin