A GFP-tagged muscleblind C protein isoform reporter construct.
Drosophila muscleblind (mbl), the ortholog of human Muscleblind-like 1 (MBNL1) gene involved in Myotonic Dystrophy (DM), gives raise to protein isoforms MblA to G. The specific functions and subcellular distribution of isoforms are still largely unknown. To overcome the lack of isoform-specific antibodies we generated transgenic flies that express a GFP:MblC fusion protein under the control of the Gal4/UAS system. The reporter fusion protein was able to functionally complement mbl loss of function mutations, demonstrating activity, and accumulated predominantly in adult muscle nuclei. The fluorescent nature of the reporter makes it appropriate for live imaging detection of MblC protein isoform.
Pascual
Maya
M
Department of Genetics, University of Valencia, Burjasot, Valencia, Spain.
Monferrer
Lidon
L
Fernandez Costa
Juan M
JM
Bargiela
Ariadna
A
Artero
Ruben
R
Llamusi
Beatriz
B
D000818:Animals; D030801:Animals, Genetically Modified; D002467:Cell Nucleus / Q000378:metabolism; D004330:Drosophila / Q000235:genetics*; D029721:Drosophila Proteins / Q000032:analysis* / Q000235:genetics* / Q000502:physiology; D017930:Genes, Reporter; D005818:Genetic Engineering / Q000379:methods; D049452:Green Fluorescent Proteins / Q000032:analysis; D009132:Muscles / Q000378:metabolism; D009687:Nuclear Proteins / Q000032:analysis* / Q000235:genetics* / Q000502:physiology; D011993:Recombinant Fusion Proteins / Q000032:analysis
D029721:Drosophila Proteins; D009687:Nuclear Proteins; D011993:Recombinant Fusion Proteins; C109271:mbl protein, Drosophila; D049452:Green Fluorescent Proteins