title: MicroRNA microarrays on archive bone marrow core biopsies of leukemias--method validation.
abstract: Due to availability of bone marrow core biopsies (CB) in many pathology laboratories, we evaluated the quality and the biological information of the miRNA profiling using 9 acute lymphoblastic leukemia (ALL) and 9 chronic myeloid leukemia (CML) matched CB and bone marrow aspirates (BA). Technical replicates showed reproducible results across platforms and clustered together in hierarchical clustering analysis; and matched samples showed similar biological content having common differentially expressed miRNAs against the same control samples. We showed, that CBs, which have underwent decalcification in addition to formalin-fixation, are suitable for miRNA profiling.
authors.affiliation: Department of Pathology, Haartman Institute and HUSLAB, University of Helsinki and Helsinki University Central Hospital, Helsinki, Finland.
authors.forename: Ioana
authors.initials: I
authors.lastname: Borze
authors.forename: Mohamed
authors.initials: M
authors.lastname: Guled
authors.forename: Suad
authors.initials: S
authors.lastname: Musse
authors.forename: Anna
authors.initials: A
authors.lastname: Raunio
authors.forename: Erkki
authors.initials: E
authors.lastname: Elonen
authors.forename: Ulla
authors.initials: U
authors.lastname: Saarinen-Pihkala
authors.forename: Marja-Liisa
authors.initials: ML
authors.lastname: Karjalainen-Lindsberg
authors.forename: Leo
authors.initials: L
authors.lastname: Lahti
authors.forename: Sakari
authors.initials: S
authors.lastname: Knuutila
mesh_terms: D001706:Biopsy; D001853:Bone Marrow; D005557:Formaldehyde; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D007938:Leukemia / Q000235:genetics*; D035683:MicroRNAs / Q000302:isolation & purification*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016707:Tissue Fixation
chemical_list: D035683:MicroRNAs; D005557:Formaldehyde