title: Modulating malignant epithelial tumor cell adhesion, migration and mechanics with nanorod surfaces.
abstract: The failure of tumor stents used for palliative therapy is due in part to the adhesion of tumor cells to the stent surface. It is therefore desirable to develop approaches to weaken the adhesion of malignant tumor cells to surfaces. We have previously developed SiO₂ coated nanorods that resist the adhesion of normal endothelial cells and fibroblasts. The adhesion mechanisms in malignant tumor cells are significantly altered from normal cells; therefore, it is unclear if nanorods can similarly resist tumor cell adhesion. In this study, we show that the morphology of tumor epithelial cells cultured on nanorods is rounded compared to flat surfaces and associated with decreased cellular stiffness and non-muscle myosin II phosphorylation. Tumor cell viability and proliferation was unchanged on nanorods. Adherent cell numbers were significantly decreased while single tumor cell motility was increased on nanorods compared to flat surfaces. Together, these results suggest that nanorods can be used to weaken malignant tumor cell adhesion, and therefore potentially improve tumor stent performance.
authors.affiliation: Department of Chemical Engineering, University of Florida, Gainesville, FL 32611, USA.
authors.forename: Jiyeon
authors.initials: J
authors.lastname: Lee
authors.forename: Byung Hwan
authors.initials: BH
authors.lastname: Chu
authors.forename: Shamik
authors.initials: S
authors.lastname: Sen
authors.forename: Anand
authors.initials: A
authors.lastname: Gupte
authors.forename: T J
authors.initials: TJ
authors.lastname: Chancellor
authors.forename: Chih-Yang
authors.initials: CY
authors.lastname: Chang
authors.forename: Fan
authors.initials: F
authors.lastname: Ren
authors.forename: Sanjay
authors.initials: S
authors.lastname: Kumar
authors.forename: Tanmay P
authors.initials: TP
authors.lastname: Lele
mesh_terms: D001696:Biomechanical Phenomena; D002277:Carcinoma / Q000378:metabolism / Q000473:pathology* / Q000628:therapy; D002448:Cell Adhesion; D002452:Cell Count; D045744:Cell Line, Tumor; D002465:Cell Movement; D006801:Humans; D055595:Mechanical Phenomena; D024462:Myosin Type II / Q000378:metabolism; D043942:Nanotubes; D015607:Stents; D013499:Surface Properties
chemical_list: D024462:Myosin Type II