Expression of aquaporin-1 in nasopharyngeal cancer tissues.
OBJECTIVE
To investigate the expression of aquaporin-1 (AQP1) in nasopharyngeal tumour and nontumour tissues.


METHODS
Thirty nasopharyngeal biopsy samples were collected and examined with both immunohistochemistry and real-time fluorescence quantitative polymerase chain reaction (RT-PCR) to detect the expression level of AQP1 in tissues.


RESULTS
Immunohistochemistry data suggested that AQP1 was expressed in both nasopharyngeal tumour and normal tissues, but tumour tissues were stained a lot stronger than nontumour tissues. RT-PCR analysis further confirmed the overexpression of AQP1 in tumour tissues; migrated tumour tissue had even higher expression.


CONCLUSIONS
The expression level of aquaporin-1 in nasopharyngeal cancer tissue could be involved in tumour migration.
Li
Qi
Q
Department of Otolaryngology-Head and Neck Surgery, and Bao Zhang: Molecular Biology Laboratory, Southern Medical University, Guangzhou, Guangdong, PR China.
Zhang
Bao
B
D000293:Adolescent; D000328:Adult; D000368:Aged; D051398:Aquaporin 1 / Q000096:biosynthesis / Q000235:genetics*; D014408:Biomarkers, Tumor / Q000096:biosynthesis / Q000235:genetics; D004273:DNA, Neoplasm / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D007828:Laryngoscopy; D008297:Male; D008875:Middle Aged; D009303:Nasopharyngeal Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D016133:Polymerase Chain Reaction; D012189:Retrospective Studies; D055815:Young Adult
D014408:Biomarkers, Tumor; D004273:DNA, Neoplasm; D051398:Aquaporin 1