Nanoscale biomolecular structures on self-assembled monolayers generated from modular pegylated disulfides.
A solid-phase synthetic strategy was developed that uses modular building blocks to prepare symmetric oligo(ethylene glycol)-terminated disulfides with a variety of lengths and terminal functionalities. The modular disulfides, composed of alkyl amino groups linked by an amide group to oligoethylene chains were used to generate self-assembled monolayers (SAMs), which were characterised to determine their applicability for biomolecular applications. X-ray photoelectron spectroscopy (XPS) of the SAMs obtained from these molecules demonstrated improved stability towards displacement by 16-hexadecanethiol, while surface plasmon resonance (SPR) analyses of SAMs prepared with the hydroxy-terminated oligoethylene disulfide showed equal resistance to non-specific protein adsorption in comparison to 11-mercaptoundecyl tri(ethylene glycol). SAMs made from these adsorbates were amenable to nanoscale patterning by scanning near-field photolithography (SNP), facilitating the fabrication of nanopatterned, protein-functionalised surfaces. Such SAMs may be further developed for bionanotechnology applications such as the fabrication of nanoscale biological arrays and sensor devices.
Wong
Lu Shin
LS
School of Chemistry & Manchester Interdisciplinary Biocentre, University of Manchester, 131 Princess Street, Manchester M1 7DN, UK.
Janusz
Stefan J
SJ
Sun
Shuqing
S
Leggett
Graham J
GJ
Micklefield
Jason
J
D004220:Disulfides / Q000737:chemistry*; D019855:Ethylene Glycol / Q000737:chemistry*; D015394:Molecular Structure; D036103:Nanotechnology; D056951:Photoelectron Spectroscopy; D011092:Polyethylene Glycols / Q000737:chemistry*; D013499:Surface Properties
D004220:Disulfides; D011092:Polyethylene Glycols; D019855:Ethylene Glycol