title: Identification of genes involved in the response of banana to crown rot disease.
abstract: Variations in banana susceptibility to crown rot disease have been observed but the molecular mechanisms underlying these quantitative host-pathogen relationships are still unknown. This study was designed to compare gene expression between crowns of banana fruit showing a high susceptibility (S(+)) and crowns showing a low susceptibility (S(-)) to the disease. Comparisons were performed at two situation times: i) between crowns (S(+) and S(-)) collected 1 h before inoculation and ii) between crowns (S+ and S-) collected 13 days after inoculation. Gene expression comparisons were performed with cDNA-amplified fragment length polymorphism (AFLP) and results were confirmed by real-time reverse-transcription polymerase chain reaction. Among genes identified as differentially expressed between S(+) and S(-) crowns, two were involved in signal transduction, three in proteolytic machinery, two had similarity to pathogenesis-related protein 14, one to a CCR4-associated factor protein, and one to a cellulose synthase. Paradoxically, the overexpression of the cellulose synthase gene was associated with banana showing a high susceptibility in both pre- and post-inoculation situations. Finally, the cDNA-AFLP identified a gene that seems to be associated with the quantitative banana responses to crown rot disease; this gene encodes a dopamine-β-monooxygenase, which is involved in the catecholamine pathway. To our knowledge, this work is the first to address both pre- and post-infection gene expression with the same host-pathogen combination and distinct susceptibility levels.
authors.affiliation: University of Liege, Gembloux Agro-Bio Tech, Plant Pathology Unit. Passage des Déportés 2, B-5030 Gembloux, Belgium. ludivine.lassois@ulg.ac.be
authors.forename: Ludivine
authors.initials: L
authors.lastname: Lassois
authors.forename: Patrick
authors.initials: P
authors.lastname: Frettinger
authors.forename: Luc
authors.initials: L
authors.lastname: de Lapeyre de Bellaire
authors.forename: Philippe
authors.initials: P
authors.lastname: Lepoivre
authors.forename: Haissam
authors.initials: H
authors.lastname: Jijakli
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis; D018076:DNA, Complementary / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D004299:Dopamine beta-Hydroxylase / Q000235:genetics; D005638:Fruit / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D020022:Genetic Predisposition to Disease; D005964:Glucosyltransferases / Q000235:genetics; D028521:Musa / Q000201:enzymology / Q000235:genetics*; D010935:Plant Diseases / Q000235:genetics*; D018515:Plant Leaves / Q000201:enzymology / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods
chemical_list: D018076:DNA, Complementary; D018744:DNA, Plant; D010940:Plant Proteins; D004299:Dopamine beta-Hydroxylase; D005964:Glucosyltransferases; C478648:cellulose synthase