[Genome sequencing and analysis of a peste des petits ruminants virus isolate, China/Tib/07].
Peste des petits ruminants virus is a member of Morbillivirus Paramyxoviridae. The complete genome of a Peste des petits ruminants virus (PPRV) isolate, China/Tib/07 was sequenced and molecular characteristics was analyzed. The internal sequences of the virus genome were amplified by RT-PCR with primers designed according to the published data in GenBank, while the sequences of the 3' and 5' ends of the genome were determined by RACE. Amplification products were directly sequenced,assembled and analyzed with DNAStar4.0. Results showed that China/Tib/07 genome consisted of 15 948 nucleotides in length, encoding six structural proteins and two non-structural proteins just like other known PPRV genomes. Phylogenetically, the virus genome shared homology of 91.6%-98.1% with Southwest Asian isolates among PPRV strains and the highest homology of 64.3% with rinderpest virus among morbillivirus members.
Liu
Wen-Hua
WH
College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China.
Bao
Jing-Yue
JY
Wu
Xiao-Dong
XD
Wang
Zhi-Liang
ZL
D000818:Animals; D001483:Base Sequence; D002681:China; D002522:Chlorocebus aethiops; D016679:Genome, Viral; D008969:Molecular Sequence Data; D029021:Peste-des-Petits-Ruminants / Q000662:veterinary* / Q000821:virology; D018107:Peste-des-petits-ruminants virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012756:Sheep; D012757:Sheep Diseases / Q000821:virology*; D014709:Vero Cells; D014764:Viral Proteins / Q000235:genetics
D014764:Viral Proteins