title: α,α'-Dihydroxyketone formation using aromatic and heteroaromatic aldehydes with evolved transketolase enzymes.
abstract: Transketolase mutants have been identified that accept aromatic acceptors with good stereoselectivities, in particular benzaldehyde for which the wild type enzyme showed no activity.
authors.affiliation: Department of Chemistry, University College London, 20 Gordon Street, London WC1H 0AJ, UK.
authors.forename: James L
authors.initials: JL
authors.lastname: Galman
authors.forename: David
authors.initials: D
authors.lastname: Steadman
authors.forename: Sarah
authors.initials: S
authors.lastname: Bacon
authors.forename: Phattaraporn
authors.initials: P
authors.lastname: Morris
authors.forename: Mark E B
authors.initials: ME
authors.lastname: Smith
authors.forename: John M
authors.initials: JM
authors.lastname: Ward
authors.forename: Paul A
authors.initials: PA
authors.lastname: Dalby
authors.forename: Helen C
authors.initials: HC
authors.lastname: Hailes
mesh_terms: D000447:Aldehydes / Q000378:metabolism; D001547:Benzaldehydes / Q000378:metabolism; D002384:Catalysis; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D007659:Ketones / Q000378:metabolism*; D009154:Mutation; D013237:Stereoisomerism; D013379:Substrate Specificity; D014174:Transketolase / Q000235:genetics / Q000378:metabolism*
chemical_list: D000447:Aldehydes; D001547:Benzaldehydes; D007659:Ketones; D014174:Transketolase; C032175:benzaldehyde