title: Detection of oseltamivir resistance during treatment of 2009 H1N1 influenza virus infection in immunocompromised patients: utility of cycle threshold values of qualitative real-time reverse transcriptase PCR.
abstract: Two immunocompromised patients with 2009 H1N1 influenza pneumonia had viral shedding for over 5 weeks despite therapy with oseltamivir. Declining or persistently low cycle threshold values noted on serial qualitative real-time reverse transcriptase PCR (rRT-PCR) of respiratory specimens implied increasing viral load and probable drug resistance. Oseltamivir resistance was later confirmed by pyrosequencing.
authors.affiliation: Division of Infectious Diseases, Beaumont Hospital, Detroit, Michigan 48201, USA.
authors.forename: Tejal
authors.initials: T
authors.lastname: Mehta
authors.forename: Eric
authors.initials: E
authors.lastname: McGrath
authors.forename: Suchitha
authors.initials: S
authors.lastname: Bheemreddy
authors.forename: Hossein
authors.initials: H
authors.lastname: Salimnia
authors.forename: Nahed
authors.initials: N
authors.lastname: Abdel-Haq
authors.forename: Jocelyn Y
authors.initials: JY
authors.lastname: Ang
authors.forename: Lawrence
authors.initials: L
authors.lastname: Lum
authors.forename: Pranatharthi
authors.initials: P
authors.lastname: Chandrasekar
authors.forename: George J
authors.initials: GJ
authors.lastname: Alangaden
mesh_terms: D000998:Antiviral Agents / Q000008:administration & dosage / Q000494:pharmacology*; D024882:Drug Resistance, Viral; D006801:Humans; D016867:Immunocompromised Host; D053118:Influenza A Virus, H1N1 Subtype / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D007251:Influenza, Human / Q000188:drug therapy* / Q000821:virology; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D053139:Oseltamivir / Q000008:administration & dosage / Q000494:pharmacology*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA; D013183:Sputum / Q000821:virology; D013997:Time Factors; D019562:Viral Load; D017201:Virus Shedding
chemical_list: D000998:Antiviral Agents; D053139:Oseltamivir