title: Therapeutic efficacies of Coriandrum sativum aqueous extract against metronidazole-induced genotoxicity in Channa punctatus peripheral erythrocytes.
abstract: Metronidazole (MTZ), a nitroimidazole drug, is primarily used as an anti-protozoan or an anti-bacterial agent in humans, although its genotoxic and carcinogenic effects have been widely reported, particularly in aquatic organisms. MTZ may induce DNA damages through single-strand breaks, modification of bases, DNA-DNA and DNA-protein cross-links, ultimately leading to apoptosis or necrosis. Here, we have assessed the genotoxicity of MTZ in the peripheral erythrocytes of Channa punctatus, using micronucleation (MN) and binucleation (BN) as genotoxicity markers. The therapeutic potential of aqueous extract of Coriandrum sativum against MTZ-induced genotoxicity has also been examined. The results show significant (P<0.05) increase in both MN and BN formation due to MTZ treatment. Such aberrations were higher in smaller fish samples for a particular dosage of MTZ, as established by correlation analysis between fish body weight and MN/BN count at P<0.05. However, such degenerative damages were found to be alleviated by a great extent due to treatment with C. sativum leaf extract. Hence, we establish that MTZ can produce considerable degrees of micronucleus and binucleus formation in peripheral erythrocytes of C. punctatus, and such deleterious effect of MTZ treatment can be mitigated by aqueous extract of C. sativum leaves.
authors.affiliation: Department of Environmental Science, University of Calcutta, Kolkata, West Bengal, India.
authors.forename: Soumendra Nath
authors.initials: SN
authors.lastname: Talapatra
authors.forename: Subham
authors.initials: S
authors.lastname: Dasgupta
authors.forename: Gunjan
authors.initials: G
authors.lastname: Guha
authors.forename: Moumita
authors.initials: M
authors.lastname: Auddy
authors.forename: Aniruddha
authors.initials: A
authors.lastname: Mukhopadhyay
mesh_terms: D000818:Animals; D016587:Antimutagenic Agents / Q000494:pharmacology*; D000994:Antitrichomonal Agents / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D001835:Body Weight / Q000187:drug effects; D002467:Cell Nucleus / Q000187:drug effects; D028526:Coriandrum / Q000737:chemistry*; D004912:Erythrocytes / Q000187:drug effects*; D007202:Indicators and Reagents; D008795:Metronidazole / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D015162:Micronucleus Tests; D010473:Perciformes / Q000502:physiology*; D010936:Plant Extracts / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry
chemical_list: D016587:Antimutagenic Agents; D000994:Antitrichomonal Agents; D007202:Indicators and Reagents; D010936:Plant Extracts; D008795:Metronidazole