Solid-state NMR characterization of gas vesicle structure.
Gas vesicles are gas-filled buoyancy organelles with walls that consist almost exclusively of gas vesicle protein A (GvpA). Intact, collapsed gas vesicles from the cyanobacterium Anabaena flos-aquae were studied by solid-state NMR spectroscopy, and most of the GvpA sequence was assigned. Chemical shift analysis indicates a coil-α-β-β-α-coil peptide backbone, consistent with secondary-structure-prediction algorithms, and complementary information about mobility and solvent exposure yields a picture of the overall topology of the vesicle subunit that is consistent with its role in stabilizing an air-water interface.
Sivertsen
Astrid C
AC
Department of Chemistry, Brandeis University, Waltham, Massachusetts, USA.
Bayro
Marvin J
MJ
Belenky
Marina
M
Griffin
Robert G
RG
Herzfeld
Judith
J
D017033:Anabaena; D005740:Gases; D057927:Hydrophobic and Hydrophilic Interactions; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D009068:Movement; D015388:Organelles / Q000737:chemistry*; D017433:Protein Structure, Secondary; D011506:Proteins / Q000737:chemistry / Q000378:metabolism; D012997:Solvents / Q000737:chemistry
D005740:Gases; D011506:Proteins; D012997:Solvents; C038524:gas vesicle protein