title: Peanut-allergic subjects and their peanut-tolerant siblings have large differences in peanut-specific IgG that are independent of HLA class II.
abstract: We enrolled 53 peanut-allergic subjects and 64 peanut-tolerant full siblings, measured peanut-specific IgG and IgE, determined HLA class II at high resolution, and analyzed DRB1 alleles by supertypes. Peanut-specific IgG and IgE were elevated in the peanut-allergic subjects (p<0.0001) but did not stratify with HLA alleles, haplotypes, or supertypes. There were no significant differences in HLA class II between the peanut-allergic and peanut-tolerant siblings but there was an increased frequency of DRB1*0803 in both sets of siblings compared to unrelated controls (p(c)=4.5×10⁻⁹). Furthermore, we identified 14 sibling pairs in which the peanut-allergic and the peanut-tolerant siblings have identical HLA class II and again found an elevation of anti-peanut IgG in the peanut-allergic subjects (p<0.0001). In conclusion, although DRB1*0803 may identify a subset of families with increased risk of peanut allergy, differences in peanut-specific immunoglobulin production between peanut-allergic subjects and their peanut-tolerant siblings are independent of HLA class II.
authors.affiliation: Division of Allergy and Clinical Immunology, Univ. of Colorado Denver, Aurora, CO 80045, USA. stephen.dreskin@ucdenver.edu
authors.forename: Stephen C
authors.initials: SC
authors.lastname: Dreskin
authors.forename: Mark T
authors.initials: MT
authors.lastname: Tripputi
authors.forename: Michael T
authors.initials: MT
authors.lastname: Aubrey
authors.forename: S Shahzad
authors.initials: SS
authors.lastname: Mustafa
authors.forename: Dan
authors.initials: D
authors.lastname: Atkins
authors.forename: Harvey L
authors.initials: HL
authors.lastname: Leo
authors.forename: Benjamin
authors.initials: B
authors.lastname: Song
authors.forename: Darcy
authors.initials: D
authors.lastname: Schlichting
authors.forename: Hanna
authors.initials: H
authors.lastname: Talwar
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.forename: Brian M
authors.initials: BM
authors.lastname: Freed
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D010367:Arachis / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006684:HLA-DR Antigens / Q000276:immunology*; D059811:HLA-DRB1 Chains; D006239:Haplotypes; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D006801:Humans; D007073:Immunoglobulin E / Q000096:biosynthesis / Q000276:immunology*; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D021183:Peanut Hypersensitivity / Q000235:genetics* / Q000276:immunology*; D012720:Severity of Illness Index; D035781:Siblings; D012882:Skin Tests; D044465:White People / Q000235:genetics*; D055815:Young Adult
chemical_list: D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains; D000949:Histocompatibility Antigens Class II; D007074:Immunoglobulin G; D007073:Immunoglobulin E