Influence of high temperature during grain filling on the accumulation of storage proteins and grain quality in rice (Oryza sativa L.).
The present study was performed to understand the effects of high temperature (HT) during filling on the expression of storage proteins and the quality of rice grains. HT (35/30 °C day/night) reduced the weight, amylose content, and flour gel consistency of grains. It increased the accumulation of all classes of storage proteins at early filling stage but decreased the accumulation of prolamins at maturation. For albumins, the expressions of cyclophilin 2, peroxiredoxin, and HSP16.9 were differentially enhanced by HT. For globulins, HT decreased the accumulation of globulin but increased that of glyoxalase I and peroxiredoxin. HT enhanced the transcription of genes for glutelins, prolamins, globulins, and protein disulfide isomerase at early filling stage but decreased the expression of these genes at a later stage. Low amounts of prolamins and globulins, as well as low pH value, were found in sound, immature, and dead kernels grown under HT. The relationships among HT, storage proteins, and grain quality are discussed.
Lin
Chin-Ju
CJ
Department of Agronomy, National Taiwan University, Taipei, Taiwan, Republic of China.
Li
Chia-Yu
CY
Lin
Shao-Kai
SK
Yang
Fan-Hsuan
FH
Huang
Ji-Jwo
JJ
Liu
Yun-Hua
YH
Lur
Huu-Sheng
HS
D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D012275:Oryza / Q000737:chemistry*; D011786:Quality Control; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055314:Seed Storage Proteins / Q000032:analysis* / Q000235:genetics; D012639:Seeds / Q000737:chemistry; D053719:Tandem Mass Spectrometry
D012333:RNA, Messenger; D055314:Seed Storage Proteins