title: Granulocyte-colony stimulating factor ameliorates irradiation-induced suppression of hippocampal neurogenesis in adult mice.
abstract: Granulocyte-colony stimulating factor (G-csf) is a member of the hematopoietic growth factor family and demonstrates neuroprotective functions in neurodegenerative diseases. This study evaluated the radioprotective effects of G-csf in the suppression of hippocampal neurogenesis in adult mice undergoing irradiation. The radioprotective effects were assessed using the terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling assay and immunohistochemical markers of neurogenesis, including the proliferating cell marker Ki-67 and the immature progenitor neuron marker doublecortin (DCX). Acute exposure to cranial irradiation (5Gy γ-rays) induced neural apoptosis and inhibited neurogenesis in the dentate gyrus (DG) of the adult mouse hippocampus. Pretreatment with G-csf (100μg/kg every 12h subcutaneously on three consecutive days) attenuated neural apoptosis and decreased the number of Ki-67- and DCX-positive cells in the DG of the irradiated mouse hippocampus. Therefore, G-csf inhibited the detrimental effects of irradiation on hippocampal neurogenesis, suggesting that G-csf administration has potential therapeutic utility in brain irradiation.
authors.affiliation: Department of Veterinary Anatomy, College of Veterinary Medicine, Chonnam National University, Gwangju 500-757, South Korea.
authors.forename: Joong-Sun
authors.initials: JS
authors.lastname: Kim
authors.forename: Miyoung
authors.initials: M
authors.lastname: Yang
authors.forename: Hyosun
authors.initials: H
authors.lastname: Jang
authors.forename: Heejin
authors.initials: H
authors.lastname: Oui
authors.forename: Sung-Ho
authors.initials: SH
authors.lastname: Kim
authors.forename: Taekyun
authors.initials: T
authors.lastname: Shin
authors.forename: Won-Suk
authors.initials: WS
authors.lastname: Jang
authors.forename: Seung-Sook
authors.initials: SS
authors.lastname: Lee
authors.forename: Changjong
authors.initials: C
authors.lastname: Moon
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000528:radiation effects; D016371:Cranial Irradiation / Q000009:adverse effects*; D018891:Dentate Gyrus / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D000089622:Doublecortin Domain Proteins; D000089662:Doublecortin Protein; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects*; D019394:Ki-67 Antigen / Q000378:metabolism; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D055495:Neurogenesis / Q000187:drug effects*; D009479:Neuropeptides / Q000378:metabolism; D018696:Neuroprotective Agents / Q000494:pharmacology*; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D011994:Recombinant Proteins
chemical_list: C000714847:Dcx protein, mouse; D000089622:Doublecortin Domain Proteins; D000089662:Doublecortin Protein; D019394:Ki-67 Antigen; D008869:Microtubule-Associated Proteins; D009479:Neuropeptides; D018696:Neuroprotective Agents; D011837:Radiation-Protective Agents; D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor