title: Identification of dispensable nucleotide sequence in 3' untranslated region of porcine reproductive and respiratory syndrome virus.
abstract: The 3' untranslated region (UTR) of porcine arterivirus genome plays a pivotal role for virus replication, yet the properties of 3' UTR remain largely undefined. We conducted site-directed mutagenesis to the 3' UTR of the type II porcine reproductive and respiratory syndrome virus (PRRSV). Serial deletions of the 3' UTR showed that at least 40 nucleotides immediately following the ORF7 stop codon were dispensable for the viability of PRRSV in cultured cells. We then constructed a chimeric PRRSV cDNA clone using type II PRRSV as the backbone containing the 3' UTR from the type I PRRSV. The chimeric virus was viable and shared similar properties with the parental virus. Our results provided the first description of the 40nt dispensable region in type I PRRSV 3' UTR, and further predicted structure demonstrated that the high-order structure of 3' UTR might play significant roles in its function.
authors.affiliation: Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Sun
authors.forename: Changlong
authors.initials: C
authors.lastname: Liu
authors.forename: Feifei
authors.initials: F
authors.lastname: Tan
authors.forename: Fei
authors.initials: F
authors.lastname: Gao
authors.forename: Ping
authors.initials: P
authors.lastname: Liu
authors.forename: Aijian
authors.initials: A
authors.lastname: Qin
authors.forename: Shishan
authors.initials: S
authors.lastname: Yuan
mesh_terms: D020413:3' Untranslated Regions; D000818:Animals; D002460:Cell Line; D050296:Microbial Viability; D016297:Mutagenesis, Site-Directed; D009690:Nucleic Acid Conformation; D019316:Porcine respiratory and reproductive syndrome virus / Q000235:genetics* / Q000502:physiology; D011995:Recombination, Genetic; D017384:Sequence Deletion; D013552:Swine; D014779:Virus Replication
chemical_list: D020413:3' Untranslated Regions