title: Construction of an artificial glutathione peroxidase active site on copolymer vesicles.
abstract: To construct an efficient GPx mimic, a novel method for preparing polymer-based vesicles carrying GPx-active sites was developed. A series of block copolymers loaded with recognition and catalytic sites were synthesized based on polystyrene-block-poly[tri(ethylene glycol) methyl ether acrylate]s (PS-PMEO(3) MAs). By altering the molar ratio of the functional copolymers, vesicles with GPx activity were obtained by self-assembly of these functional copolymers through blending. The optimum GPx mimic constructed by the blending process exhibited high catalytic activity and acted as a real catalyst with typical saturation kinetics behavior. The method may be of benefit for designing other enzyme mimics and may cast a light on constructing other biologically related functional nanoparticles.
authors.affiliation: State Key Laboratory of Supramolecular Structure and Materials, Jilin Univerity, Changchun 130012, People's Republic of China.
authors.forename: Yanzhen
authors.initials: Y
authors.lastname: Yin
authors.forename: Xin
authors.initials: X
authors.lastname: Huang
authors.forename: Chunyan
authors.initials: C
authors.lastname: Lv
authors.forename: Liang
authors.initials: L
authors.lastname: Wang
authors.forename: Shuangjiang
authors.initials: S
authors.lastname: Yu
authors.forename: Quan
authors.initials: Q
authors.lastname: Luo
authors.forename: Jiayun
authors.initials: J
authors.lastname: Xu
authors.forename: Junqiu
authors.initials: J
authors.lastname: Liu
mesh_terms: D020134:Catalytic Domain / Q000235:genetics*; D005979:Glutathione Peroxidase / Q000138:chemical synthesis* / Q000235:genetics*; D007700:Kinetics; D015394:Molecular Structure; D011092:Polyethylene Glycols / Q000138:chemical synthesis / Q000737:chemistry; D011109:Polymethacrylic Acids / Q000138:chemical synthesis / Q000737:chemistry; D011137:Polystyrenes / Q000737:chemistry; D015202:Protein Engineering / Q000379:methods*; D013691:Tellurium; D022161:Transport Vesicles / Q000378:metabolism
chemical_list: D011109:Polymethacrylic Acids; D011137:Polystyrenes; C466018:poly(triethylene glycol monomethacrylate); D011092:Polyethylene Glycols; D005979:Glutathione Peroxidase; D013691:Tellurium