Influence of Moringa oleifera on pharmacokinetic disposition of rifampicin using HPLC-PDA method: a pre-clinical study.
The influence of active fraction isolated from pods of an indigenous plant, Moringa oleifera (MoAF) was studied on the pharmacokinetic profile of the orally administered frontline anti-tuberculosis drug rifampicin (20 mg/kg b.w.) in Swiss albino mice. The antibiotic rifampicin alone and in combination with MoAF (0.1 mg/kg b.w.) was administered orally and heparanized blood samples were collected from the orbital plexus of mice for plasma separation at 0, 1, 2, 3, 4 and 5 h, post treatment. Plasma rifampicin concentration, pharmacokinetic parameters and drug metabolizing enzyme (cytochrome P-450) activity were determined. The pharmacokinetic data revealed that MoAF-treated animals had significantly increased rifampicin plasma concentration, C(max), K(el), t(½(a)), t(½(el)), K(a) and AUC as well as inhibited rifampicin-induced cytochrome P-450 activity. In conclusion, the result of this study suggested that the bioavailability-enhancing property of MoAF may help to lower the dosage level and shorten the treatment course of rifampicin.
Pal
Anirban
A
Division of Molecular Bioprospection, Central Institute of Medicinal and Aromatic Plants, CSIR, Lucknow, India. a.pal@cimap.res.in
Bawankule
Dnyaneshwar Umrao
DU
Darokar
Mahendra Pandurang
MP
Gupta
Subhash Chandra
SC
Arya
Jai Shankar
JS
Shanker
Karuna
K
Gupta
Madan Mohan
MM
Yadav
Narayan Prasad
NP
Singh Khanuja
Suman Preet
SP
D000818:Animals; D019540:Area Under Curve; D001682:Biological Availability; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D004353:Drug Evaluation, Preclinical; D004357:Drug Synergism; D005260:Female; D008297:Male; D051379:Mice; D008862:Microsomes, Liver / Q000737:chemistry / Q000201:enzymology; D029588:Moringa oleifera / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*; D012293:Rifampin / Q000097:blood / Q000493:pharmacokinetics*
D010936:Plant Extracts; D003577:Cytochrome P-450 Enzyme System; D012293:Rifampin