Cytotoxic 3,4-seco-Atisane Diterpenoids from Croton barorum and Croton goudotii.
In a screening program directed to the discovery of new anticancer agents from Madagascan plants, ethyl acetate extracts of Croton barorum and C. goudotii showed strong cytotoxic activity, with 100% inhibition at 10 μg/mL in a primary screen using the murine lymphocytic leukemia P388 cell line. Bioassay-guided fractionation led to the isolation of two new 3,4-seco-atisane diterpenoids, crotobarin (1) and crotogoudin (2). Their structures were elucidated by spectroscopic data interpretation. Compounds 1 and 2 produced a net progression in the number of cells arrested at the G2/M growth stage in the cell cycle of the K562 human leukemia cell line at 4 μM.
Rakotonandrasana
Olga L
OL
Laboratoire de Biothérapeutique, Institut Malgache des Recherches Appliquées, BP 3833, 101 Antananarivo, Madagascar.
Raharinjato
Fanja H
FH
Rajaonarivelo
Mialy
M
Dumontet
Vincent
V
Martin
Marie-Thérèse
MT
Bignon
Jérôme
J
Rasoanaivo
Philippe
P
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D029802:Croton / Q000737:chemistry* / Q000235:genetics; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D020014:K562 Cells; D008270:Madagascar; D051379:Mice; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular
D000972:Antineoplastic Agents, Phytogenic; D004224:Diterpenes; C000600506:crotobarin; C000600507:crotogoudin