title: Plasma miR-216a as a potential marker of pancreatic injury in a rat model of acute pancreatitis.
abstract: OBJECTIVE
  To study the potential value and specificity of plasma miR-216a as a marker for pancreatic injury.
  
  
  METHODS
  Two rat models were applied in this article: L-arginine-induced acute pancreatitis was used as one model to explore the potential value of plasma miR-216a for detection of pancreatic injury; nonlethal sepsis induced in rats by single puncture cecal ligation and puncture (CLP) was used as the other model to evaluate the specificity of plasma miR-216a compared with two commonly used markers (amylase and lipase) for acute pancreatitis. Plasmas were sampled from rats at indicated time points and total RNA was isolated. Real-Time Quantitative reverse transcriptase-polymerase chain reaction was used to quantify miR-216a in plasmas.
  
  
  RESULTS
  In the acute pancreatitis model, among five time points at which plasmas were sampled, miR-216a concentrations were significantly elevated 24 h after arginine administration and remained significantly increased until 48 h after operation (compared with 0 h time point, P < 0.01, Kruskal-Wallis Test). In the CLP model, plasma amylase and lipase, two commonly used biomarkers for acute pancreatitis, were significantly elevated 24 h after operation (compared with 0 h time point, P < 0.01 and 0.05 respectively, Pairwise Bonferroni corrected t-tests), while miR-216a remained undetectable among four tested time points.
  
  
  CONCLUSIONS
  Our article showed for the first time that plasma miR-216a might serve as a candidate marker of pancreatic injury with novel specificity.
authors.affiliation: Department of Gastroenterology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
authors.forename: Xiang-Yu
authors.initials: XY
authors.lastname: Kong
authors.forename: Yi-Qi
authors.initials: YQ
authors.lastname: Du
authors.forename: Lei
authors.initials: L
authors.lastname: Li
authors.forename: Jian-Qiang
authors.initials: JQ
authors.lastname: Liu
authors.forename: Guo-Kun
authors.initials: GK
authors.lastname: Wang
authors.forename: Jia-Qi
authors.initials: JQ
authors.lastname: Zhu
authors.forename: Xiao-Hua
authors.initials: XH
authors.lastname: Man
authors.forename: Yan-Fang
authors.initials: YF
authors.lastname: Gong
authors.forename: Li-Ning
authors.initials: LN
authors.lastname: Xiao
authors.forename: Yong-Zhi
authors.initials: YZ
authors.lastname: Zheng
authors.forename: Shang-Xin
authors.initials: SX
authors.lastname: Deng
authors.forename: Jun-Jun
authors.initials: JJ
authors.lastname: Gu
authors.forename: Zhao-Shen
authors.initials: ZS
authors.lastname: Li
mesh_terms: D000681:Amylases / Q000097:blood; D000818:Animals; D001120:Arginine / Q000494:pharmacology; D015415:Biomarkers / Q000097:blood*; D004195:Disease Models, Animal; D006801:Humans; D008049:Lipase / Q000097:blood; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D010179:Pancreas / Q000187:drug effects / Q000293:injuries*; D010195:Pancreatitis / Q000097:blood* / Q000139:chemically induced / Q000235:genetics*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D015415:Biomarkers; D035683:MicroRNAs; D001120:Arginine; D008049:Lipase; D000681:Amylases