title: CGGBP1 is a nuclear and midbody protein regulating abscission.
abstract: Abscission marks the completion of cell division and its failure is associated with delayed cytokinesis and even tetraploidization. Aberrant abscission and consequential ploidy changes can underlie various diseases including cancer. Midbody, a transient structure formed in the intercellular bridge during telophase, contains several proteins including Aurora kinase B (AURKB), which participate in abscission. We report here an unexpected expression pattern and function of the transcription repressor protein CGG triplet repeat-binding protein 1 (CGGBP1), in normal human fibroblasts. We show that CGGBP1, a chromatin-associated protein, trans-localizes to spindle midzone and midbodies in a manner similar to that of AURKB. CGGBP1 depletion resulted in a cell cycle block at G2, characterized by failure of cells to undergo mitosis and also reduced entry into S phase. Consistent with its presence in the midbodies, live microscopy showed that CGGBP1 deficiency caused mitotic failure at abscission resulting in tetraploidy, which could be rescued by CGGBP1 overexpression. These results show that CGGBP1 is a bona fide midbody protein required for normal abscission and mitosis in general.
authors.affiliation: Department of Genetics and Pathology, Uppsala University, Uppsala, Sweden. umashankar.singh@genpat.uu.se
authors.forename: Umashankar
authors.initials: U
authors.lastname: Singh
authors.forename: Bengt
authors.initials: B
authors.lastname: Westermark
mesh_terms: D064107:Aurora Kinase B; D064127:Aurora Kinases; D002453:Cell Cycle / Q000502:physiology; D018797:Cell Cycle Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002455:Cell Division / Q000502:physiology; D002467:Cell Nucleus / Q000737:chemistry / Q000378:metabolism*; D002478:Cells, Cultured; D048749:Cytokinesis / Q000502:physiology*; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D006801:Humans; D008297:Male; D008938:Mitosis / Q000502:physiology; D015388:Organelles / Q000737:chemistry / Q000378:metabolism*; D011003:Ploidies; D017346:Protein Serine-Threonine Kinases / Q000502:physiology; D016196:S Phase / Q000502:physiology; D012867:Skin / Q000166:cytology; D008941:Spindle Apparatus / Q000378:metabolism; D013692:Telophase / Q000502:physiology; D057891:Tetraploidy
chemical_list: C106905:CGGBP1 protein, human; D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; C576921:AURKB protein, human; D064107:Aurora Kinase B; D064127:Aurora Kinases; D017346:Protein Serine-Threonine Kinases