title: CG base pair recognition within DNA triple helices by modified N-methylpyrrolo-dC nucleosides.
abstract: 3-Aminophenyl-modified analogues of the bicyclic nucleoside N-methyl-3H-pyrrolo[2,3-d]pyrimidin-2(7H)-one were synthesised and incorporated directly into triplex-forming oligonucleotides in order to utilise their extended hydrogen bonding motif for recognition of the CG base pair. All analogues demonstrated strong binding affinity and very good selectivity for CG from pH 6.2 to 7.0; a marked improvement on previous modifications.
authors.affiliation: School of Chemistry, University of Southampton, Highfield, Southampton, SO17 1BJ, UK.
authors.forename: Simon R
authors.initials: SR
authors.lastname: Gerrard
authors.forename: Mastoura M
authors.initials: MM
authors.lastname: Edrees
authors.forename: Imenne
authors.initials: I
authors.lastname: Bouamaied
authors.forename: Keith R
authors.initials: KR
authors.lastname: Fox
authors.forename: Tom
authors.initials: T
authors.lastname: Brown
mesh_terms: D020029:Base Pairing / Q000235:genetics*; D004247:DNA / Q000737:chemistry*; D005453:Fluorescence; D006863:Hydrogen-Ion Concentration / Q000528:radiation effects; D008958:Models, Molecular; D009690:Nucleic Acid Conformation / Q000528:radiation effects; D009691:Nucleic Acid Denaturation / Q000528:radiation effects; D009705:Nucleosides / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry*; D014466:Ultraviolet Rays
chemical_list: D009705:Nucleosides; D011758:Pyrroles; D004247:DNA