[Transient expression and identification of gene P and NP of NDV LaSota strain in two different cells].
OBJECTIVE
To Construction of P and NP genes eukaryotic expression vectors of Newcastle Disease Virus LaSota strain,study its reverse genetics and functional genome of NDV.


METHODS
P, NP genes were amplified and cloned into pGEM-T easy vector and then subcloned into pcDNA3.1 (+) expression vector respectively, the recombinant plasmids were named pcDNA3.1 (+)-P and pcDNA3.1 (+)-NP, Recombinant plasmids were transfected into 293 and BHK-21 cells respectively and were detected using IE and Western blot analysis.


RESULTS
Expression of P, NP genes were detected and confirmed by the IE and WB analysis.


CONCLUSIONS
The recombinant eukaryotic plasmids pcDNA3. 1(+)-P, pcDNA3.1 (+)-NP were expressed in 293 and BHK-21 cells successfully. This research may be helpful for further study of reverse genetics and functional genome of NDV.
Zhou
Yuan
Y
The College of Life Science and Bio-engineering, Beijing University of Technology, Beijing 100124, China.
Jia
Run-Qing
RQ
Teng
Zhi-Ping
ZP
Zhang
Xiao-Mei
XM
Zeng
Yi
Y
D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D006801:Humans; D009522:Newcastle disease virus / Q000235:genetics* / Q000378:metabolism; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins / Q000235:genetics* / Q000378:metabolism; D010750:Phosphoproteins / Q000235:genetics* / Q000378:metabolism; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism
D019590:Nucleocapsid Proteins; D009698:Nucleoproteins; C055691:P phosphoprotein, Newcastle disease virus; D010750:Phosphoproteins; D014764:Viral Proteins; C409209:nucleoprotein, Newcastle disease virus