title: [Sequence the complete sequence of bocavirus I with SISPA-PCR].
abstract: OBJECTIVE
  To sequence the complete sequence of bocavirus I with sequence independent single primer amplification (SISPA-PCR).
  
  
  METHODS
  To exclude the co-effection samples, all clinical samples of diarrhea cases were screened with special primers of rotavirus, astrovirus, adenovirus, calicivirus and bocavirus I. The virus were enriched through ultracentrifugation. Other nucleic acids, such as human and bacteria genomes, were degradated by DNase I and RNase. DNA of bocavirus was Amplificated with SISPA-PCR, then purificated, cloned and sequenced. The sequences were alighmented in nr with blastn and assembled with DNAstar.
  
  
  RESULTS
  A 4834bp sequence of bocavirus I were assembled.
  
  
  CONCLUSIONS
  SISPA-PCR is an economical and efficient technique for sequence a virus complete genome.
authors.affiliation: Chinese Center for Disease Control and Prevention, Beijing 100052, China.
authors.forename: Jin-Song
authors.initials: JS
authors.lastname: Li
authors.forename: Wei-Xia
authors.initials: WX
authors.lastname: Cheng
authors.forename: Dong-Ping
authors.initials: DP
authors.lastname: Yao
authors.forename: Bei
authors.initials: B
authors.lastname: Lan
authors.forename: Jie-Mei
authors.initials: JM
authors.lastname: Yu
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.forename: Yong-Qing
authors.initials: YQ
authors.lastname: Li
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhang
authors.forename: Miao
authors.initials: M
authors.lastname: Jin
authors.forename: Zi-Qian
authors.initials: ZQ
authors.lastname: Xu
authors.forename: Dan-Di
authors.initials: DD
authors.lastname: Li
authors.forename: Zhao-Jun
authors.initials: ZJ
authors.lastname: Duan
mesh_terms: D001483:Base Sequence; D052660:Bocavirus / Q000235:genetics* / Q000302:isolation & purification; D017931:DNA Primers / Q000235:genetics*; D003967:Diarrhea / Q000821:virology*; D016679:Genome, Viral; D006801:Humans; D010322:Parvoviridae Infections / Q000821:virology*; D016133:Polymerase Chain Reaction
chemical_list: D017931:DNA Primers