Medium optimization based on the metabolic-flux spectrum of recombinant Escherichia coli for high expression of human-like collagen II.
Recombinant Escherichia coli BL21 was used to produce human-like collagen II in fed-batch cultivation. By performing MFA (metabolic-flux analysis), the carbon/nitrogen molar ratios in both the batch and feeding media were optimized for high-level production of human-like collagen II. Three carbon/nitrogen molar ratios in both the batch and feeding media were used in the present study, and the MFA results showed that the optimal carbon/nitrogen molar ratios for the batch and feeding media were 2.36:1 and 5.12:1 respectively, yielding the highest dry-cell density (67.2 g/l dry cell weight) and human-like collagen production (10.8 g/l).
Guo
Jiaqing
J
School of Chemical Engineering, Northwest University, Taibai North Road 229, Xi'an 710069, People's Republic of China.
Luo
Yan'e
Y
Fan
Daidi
D
Yang
Bo
B
Gao
Pengfei
P
Ma
Xiaoxuan
X
Zhu
Chenhui
C
D001709:Biotechnology / Q000379:methods*; D003094:Collagen / Q000096:biosynthesis* / Q000235:genetics; D003470:Culture Media / Q000737:chemistry* / Q000494:pharmacology*; D004734:Energy Metabolism / Q000187:drug effects*; D004926:Escherichia coli / Q000166:cytology / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D006801:Humans
D003470:Culture Media; D003094:Collagen