Neurocytoprotective effects of the bioactive constituents of Pueraria thomsonii in 6-hydroxydopamine (6-OHDA)-treated nerve growth factor (NGF)-differentiated PC12 cells.
Chronic neurodegenerative disorders are having an increasing impact on public health as human longevity increases. Parkinson's disease (PD) is a degenerative disorder of the central nervous system and is characterized by motor system disorders resulting in loss of dopamine-producing brain cells. Pueraria thomsonii Benth. (Fabaceae) is an herbal medicine that has traditionally been used as an antipyretic agent. In the present study, the active constituents, daidzein and genistein, were isolated from P. thomsonii. Both compounds exhibited neurocytoprotective effects against 6-hydroxydopamine (6-OHDA)-induced cytotoxicity in nerve growth factor (NGF)-differentiated PC12 cells. Neither daidzein nor genistein affected 6-OHDA-induced cellular reactive oxygen species (ROS) generation according to flow cytometric analysis. Rather, they inhibited caspase-8 and partially inhibited caspase-3 activation, providing a protective mechanism against 6-OHDA-induced cytotoxicity in NGF-differentiated PC12 cells. The present results imply that daidzein and genistein may be useful in the development of future strategies for the treatment of PD.
Lin
Chien-Min
CM
Graduate Institute of Clinical Medicine, Taipei Medical University, Taipei 110, Taiwan.
Lin
Rong-Dih
RD
Chen
Shui-Tein
ST
Lin
Yi-Pei
YP
Chiu
Wen-Ta
WT
Lin
Jia-Wei
JW
Hsu
Feng-Lin
FL
Lee
Mei-Hsien
MH
D000818:Animals; D061945:Caspase Inhibitors; D005434:Flow Cytometry; D019833:Genistein / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D007529:Isoflavones / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D015394:Molecular Structure; D020932:Nerve Growth Factor / Q000187:drug effects / Q000378:metabolism*; D016627:Oxidopamine / Q000494:pharmacology*; D016716:PC12 Cells; D029908:Pueraria / Q000737:chemistry*; D051381:Rats; D013624:Taiwan
D061945:Caspase Inhibitors; D007529:Isoflavones; C004742:daidzein; D016627:Oxidopamine; D020932:Nerve Growth Factor; D019833:Genistein