Exercise improves bladder function in diabetic mice.
OBJECTIVE
We determined the effect of exercise on bladder dysfunction and voiding frequency in db/db mice.


METHODS
Diabetic db/db female mice (BKS.Cg-Dock7m +/+ Leprdb/J strain) and their age-matched wild-type controls (WT) were equally divided into sedentary and exercise groups. Mice were exercised for 1 hr everyday for 8 weeks (speed of 5.2 m/min). We performed a voiding pattern test, cystometric analysis and reactivity of isolated bladder strips in WT and db/db mice, both sedentary and exercised.


RESULTS
Diabetes increased the frequency of voiding, bladder capacity, and residual volume. Exercise decreased voiding frequency in db/db mice; voiding frequency was 5.8 ± 0.5 (db/db exercise) versus 10.8 ± 1.1 (db/db control, P < 0.001). In cystometric analysis, the bladder capacity of db/db sedentary mice was 0.27 ± 0.05 ml and was 0.14 ± 0.02 ml in the db/db exercise group (P < 0.05), whereas the residual volume was 0.2 ± 0.03 ml in db/db sedentary mice and 0.06 ± 0.02 ml in db/db Ex mice. Isolated strips of bladder muscle from sedentary db/db mice were more responsive to carbachol than strips from db/db exercise mice. Exercise did not improve the urodynamic properties of WT mice, both sedentary and exercised.


CONCLUSIONS
Exercise improves bladder function in diabetic mice by reducing voiding frequency and improving urodynamic parameters.
Vadhavkar
Manasi
M
Department of Experimental Medicine, Faculty of Medicine, University of British Columbia, Vancouver, Canada.
Golbidi
Saeid
S
Sea
Jason
J
Longpre
Michelle
M
Stothers
Lynn
L
Laher
Ismail
I
D000818:Animals; D003920:Diabetes Mellitus / Q000503:physiopathology* / Q000628:therapy*; D004195:Disease Models, Animal; D005080:Exercise Test; D005081:Exercise Therapy / Q000379:methods*; D005260:Female; D051379:Mice; D016896:Treatment Outcome; D014554:Urination; D014563:Urodynamics