Anti-inflammatory effect of hydrogen-rich saline in a rat model of regional myocardial ischemia and reperfusion.
BACKGROUND
To study the possible anti-inflammatory effect of hydrogen-rich saline (H(2) saline) on rat hearts with regional myocardial ischemia and reperfusion (I/R).


METHODS
Sixty-six rats were equally randomized to three groups: sham-operated group, I/R group (control group) and I/R plus H(2) saline treatment group. Myocardial I/R was established by occlusion of the left anterior descending (LAD) coronary artery for 30 min and reperfusion for 24 h.


RESULTS
H(2) saline treatment attenuated I/R-induced cardiac cell apoptosis, presenting as significant improvement of heart function parameters 24h after reperfusion, including left ventricular systolic pressure (LVSP), left ventricular diastolic pressure (LVEDP), +(dP/dt)max and -(dP/dt)max. It also decreased neutrophil infiltration, 3-nitrotyrosine level, expression of intercellular adhesion molecule 1(ICAM-1) and myeloperoxidase (MPO) activity in the area at risk zones (AAR) of rat hearts subjected to regional myocardial I/R, and attenuated the increase of I/R induced proinflammatory cytokine tumor necrosis factor-α (TNF-a) and interleukin-1β (IL-1b) levels in the AAR.


CONCLUSIONS
H(2) saline has an anti-inflammatory effect on rat hearts with regional myocardial I/R.
Zhang
Yufeng
Y
Department of Cardiothoracic Surgery, Changhai Hospital, Second Military Medical University, Shanghai 200433, PR China.
Sun
Qiang
Q
He
Bin
B
Xiao
Jian
J
Wang
Zhinong
Z
Sun
Xuejun
X
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage*; D002316:Cardiotonic Agents / Q000008:administration & dosage; D004195:Disease Models, Animal; D006859:Hydrogen / Q000008:administration & dosage*; D008297:Male; D017202:Myocardial Ischemia / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology*; D015425:Myocardial Reperfusion / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012965:Sodium Chloride / Q000008:administration & dosage*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D002316:Cardiotonic Agents; D012965:Sodium Chloride; D006859:Hydrogen