title: Vascular endothelial growth factor gene polymorphisms and ovarian cancer survival.
abstract: OBJECTIVE
  We sought to evaluate the effect of polymorphisms in the VEGF (Vascular Endothelial Growth Factor) gene on overall survival in ovarian cancer patients.
  
  
  METHODS
  A sample of 319 women diagnosed with primary invasive epithelial ovarian cancer in Australia between 1985 and 1997, recruited as incident cases, were genotyped for four VEGF single nucleotide polymorphisms (three tagSNPs and one functional SNP) using the Sequenom MassARRAY platform. A SNP found to be associated with ovarian cancer survival in this sample set was then evaluated in two independent datasets in an attempt to replicate the association.
  
  
  RESULTS
  VEGF tagSNPs rs3025033 and rs2146323 were not associated with ovarian cancer survival in the Australian sample. Ovarian cancer patients homozygous for tagSNP rs833068 or the functional SNP rs2010963 displayed significantly shortened overall survival in the Australian sample (HR 2.09, 95% CI 1.16-3.78), an effect most apparent in the first 5years after diagnosis. This association was not replicated in two independent datasets.
  
  
  CONCLUSIONS
  Findings from this study provide no evidence that rs3025033 and rs2146323 VEGF polymorphisms are associated with ovarian cancer survival. Although homozygous carriers of the tagSNP rs833068 experienced significantly worse survival in our Australian dataset, we were unable to replicate this in two independent datasets.
authors.affiliation: Molecular Cancer Epidemiology, Queensland Institute of Medical Research, P.O. Royal Brisbane Hospital, Herston, Brisbane, QLD 4029, Australia.
authors.forename: Felicity
authors.initials: F
authors.lastname: Lose
authors.forename: Christina M
authors.initials: CM
authors.lastname: Nagle
authors.forename: Tracy
authors.initials: T
authors.lastname: O'Mara
authors.forename: Jyotsna
authors.initials: J
authors.lastname: Batra
authors.forename: Kelly L
authors.initials: KL
authors.lastname: Bolton
authors.forename: Honglin
authors.initials: H
authors.lastname: Song
authors.forename: Susan J
authors.initials: SJ
authors.lastname: Ramus
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Gentry-Maharaj
authors.forename: Usha
authors.initials: U
authors.lastname: Menon
authors.forename: Simon A
authors.initials: SA
authors.lastname: Gayther
authors.forename: Paul D P
authors.initials: PD
authors.lastname: Pharoah
authors.forename: Mary-Anne
authors.initials: MA
authors.lastname: Kedda
authors.forename: Amanda B
authors.initials: AB
authors.lastname: Spurdle
mesh_terms: D000328:Adult; D000368:Aged; D005260:Female; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D009367:Neoplasm Staging; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000473:pathology; D020641:Polymorphism, Single Nucleotide; D015996:Survival Rate; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics*
chemical_list: C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A