Hepatoprotective treatment attenuates oxidative damages induced by carbon tetrachloride in rats.
The present study evaluated the hepatoprotective effect of an N-acetyl dl-methionine+choline chloride+caffeine+thiamine hydrochloride+nicotinamide+pyridoxine hydrochloride compound at doses of 0.2, 0.6 and 1.0 mL/kg of b.w., and the assessment was done by the investigation of serum-enzymatic activity, metabolic functions of the liver and histophatological changes in female Wistar rats, which were subjected to experimental intoxication with CCl(4). One hundred and nineteen rats were randomly distributed into 17 groups, performing five different treatments, being evaluated seven animals per treatment in four periods: 2, 4, 6 and 8 days after CCl(4)-induced intoxication. Treated rats with the hepatoprotective medicine (HM) presented a significant reduction in infiltration of inflammatory cells, steatosis, necrosis and liver congestion when compared to non-treated rats (control). Beside these results, the treatment showed a positive effect on circulatory alterations in the intoxicated animals, with reduction of spleen and renal congestion, as well as, promotion of a significant improvement in ALT, AST, LDH, ALP, GGT enzymatic serum activity reduction and in recovering liver function regarding the metabolism of urea, triglycerides and glucose. These findings indicate therapeutic usefulness of the compound when administered at dose 0.6 and 1.0 mL/kg of b.w. in female Wistar rats.
de Andrade Belo
Marco Antonio
MA
College of Veterinary Medicine, Laboratory of Pharmacology and Clinical Pathology, Camilo Castelo Branco University (Unicastelo), Descalvado/SP, Brazil. maabelo@hotmail.com
Soares
Vando Edésio
VE
de Souza
Luciano Melo
LM
da Rosa Sobreira
Márcia Ferreira
MF
Cassol
Daniela Miyasaka Silveira
DM
Toma
Sandra Barioni
SB
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002110:Caffeine / Q000008:administration & dosage*; D002251:Carbon Tetrachloride / Q000633:toxicity*; D056486:Chemical and Drug Induced Liver Injury / Q000473:pathology / Q000517:prevention & control*; D005260:Female; D008715:Methionine / Q000008:administration & dosage*; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D014803:Vitamin B Complex / Q000008:administration & dosage*
D000975:Antioxidants; D014803:Vitamin B Complex; D002110:Caffeine; D008715:Methionine; D002251:Carbon Tetrachloride