title: Jeotgalicoccus nanhaiensis sp. nov., isolated from intertidal sediment, and emended description of the genus Jeotgalicoccus.
abstract: A novel non-sporulating, non-motile, catalase- and oxidase-positive, strictly aerobic, Gram-positive-staining coccus, strain JSM 077023(T), was isolated from an intertidal sediment sample collected from Naozhou Island in the South China Sea, China. Growth occurred in the presence of 0.5-25 % (w/v) NaCl [optimum, 2-5 % (w/v) NaCl] and at pH 5.5-10.5 (optimum, pH 7.0-8.0) and at 4-45 °C (optimum, 30-35 °C). The major amino acid constituents of the cell wall were alanine, glycine and lysine. The major cellular fatty acids were anteiso-C₁₅:₀ and iso-C₁₅:₀. The strain contained MK-7 and MK-6 as the predominant respiratory quinones and diphosphatidylglycerol, phosphatidylglycerol and an unidentified phospholipid as the polar lipids. The genomic DNA G+C content of strain JSM 077023(T) was 41.3 mol%. A phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain JSM 077023(T) should be assigned to the genus Jeotgalicoccus and was most closely related to the type strains of Jeotgalicoccus halotolerans (sequence similarity 99.0 %) and Jeotgalicoccus aerolatus (99.0 %), followed by Jeotgalicoccus coquinae (98.6 %) and Jeotgalicoccus psychrophilus (97.4 %). 16S rRNA gene sequence similarities of less than 97 % were observed with other species of the genus Jeotgalicoccus. Levels of DNA-DNA relatedness between strain JSM 077023(T) and the type strains of J. halotolerans, J. aerolatus, J. coquinae and J. psychrophilus ranged from 36.8 to 22.7 %. The combination of phylogenetic analysis, DNA-DNA relatedness values, phenotypic characteristics and chemotaxonomic data supported the suggestion that strain JSM 077023(T) represents a novel species of the genus Jeotgalicoccus, for which the name Jeotgalicoccus nanhaiensis sp. nov. is proposed. The type strain is JSM 077023(T) ( = DSM 23006(T) = KCTC 13714(T)). An emended description of the genus Jeotgalicoccus is also presented.
authors.affiliation: College of Biology and Environmental Sciences, Jishou University, Jishou 416000, PR China.
authors.forename: Zhu-Xiang
authors.initials: ZX
authors.lastname: Liu
authors.affiliation: College of Biology and Environmental Sciences, Jishou University, Jishou 416000, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Chen
authors.affiliation: The Key Laboratory for Microbial Resources of the Ministry of Education, PR China, and Laboratory for Conservation and Utilization Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
authors.forename: Shu-Kun
authors.initials: SK
authors.lastname: Tang
authors.affiliation: Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, PR China.|The Key Laboratory for Microbial Resources of the Ministry of Education, PR China, and Laboratory for Conservation and Utilization Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
authors.forename: Yu-Qin
authors.initials: YQ
authors.lastname: Zhang
authors.affiliation: College of Biology and Environmental Sciences, Jishou University, Jishou 416000, PR China.
authors.forename: Jian-Wu
authors.initials: JW
authors.lastname: He
authors.affiliation: College of Biology and Environmental Sciences, Jishou University, Jishou 416000, PR China.
authors.forename: Qi-Hui
authors.initials: QH
authors.lastname: Chen
authors.affiliation: Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, PR China.|The Key Laboratory for Microbial Resources of the Ministry of Education, PR China, and Laboratory for Conservation and Utilization Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Li
authors.affiliation: The Key Laboratory for Microbial Resources of the Ministry of Education, PR China, and Laboratory for Conservation and Utilization Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.|College of Biology and Environmental Sciences, Jishou University, Jishou 416000, PR China.
authors.forename: Yi-Guang
authors.initials: YG
authors.lastname: Chen
mesh_terms: D000332:Aerobiosis; D000596:Amino Acids / Q000032:analysis; D001482:Base Composition; D002374:Catalase / Q000378:metabolism; D002473:Cell Wall / Q000737:chemistry; D002681:China; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D019015:Geologic Sediments / Q000382:microbiology*; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000378:metabolism; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism; D013171:Spores, Bacterial / Q000166:cytology; D040141:Staphylococcaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D013696:Temperature
chemical_list: D000596:Amino Acids; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010743:Phospholipids; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride; D010088:Oxidoreductases; D002374:Catalase