Depletion of TGF-β from fetal bovine serum.
TGF-β is one of the key cytokines controlling immune responses. Measuring TGF-β from culture supernatants in vitro is an important index of immune function. However, fetal bovine serum (FBS) contains a high level of latent TGF-β that often hampers measuring T cell-derived TGF-β in culture using FBS-supplemented medium. In this report, we generated anti-latency associated peptide (LAP) monoclonal antibodies which cross-react with bovine LAP, and developed a protocol to deplete TGF-β from FBS. This provides the ability to reliably quantify TGF-β in vitro without relying on serum-free media which do not support growth of murine T cells.
Oida
Takatoku
T
Center for Neurologic Diseases, Brigham and Women's Hospital, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, United States.
Weiner
Howard L
HL
D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000276:immunology; D002417:Cattle; D002460:Cell Line; D005312:Fetal Blood / Q000737:chemistry; D051379:Mice; D044967:Serum / Q000737:chemistry*; D016212:Transforming Growth Factor beta / Q000737:chemistry* / Q000276:immunology
D000911:Antibodies, Monoclonal; D016212:Transforming Growth Factor beta