title: A yeast bioassay for androgenic and anti-androgenic compounds based on the NH2- and COOH-terminal interaction of androgen receptor.
abstract: Androgenic compounds induce an interaction between the NH(2)- and COOH-terminal regions (N-C interaction) of androgen receptor (AR). We describe a rapid yeast bioassay for androgenic and anti-androgenic compounds based on androgen-dependent β-catenin-enhanced N-C interaction. The bioassay was also effective at detecting compounds that inhibit the N-C interaction in ways that do not involve binding to the ligand-binding domain.
authors.affiliation: Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Osaka, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Ogawa
authors.forename: Ryoichi
authors.initials: R
authors.lastname: Yamaji
authors.forename: Takakazu
authors.initials: T
authors.lastname: Mitani
authors.forename: Yohei
authors.initials: Y
authors.lastname: Murata
authors.forename: Motoyuki
authors.initials: M
authors.lastname: Nakao
authors.forename: Naoki
authors.initials: N
authors.lastname: Harada
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Nakano
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Inui
mesh_terms: D000726:Androgen Antagonists / Q000032:analysis*; D000728:Androgens / Q000032:analysis*; D000818:Animals; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D006801:Humans; D011944:Receptors, Androgen / Q000737:chemistry* / Q000378:metabolism; D015003:Yeasts; D051176:beta Catenin
chemical_list: D000726:Androgen Antagonists; D000728:Androgens; D011944:Receptors, Androgen; D051176:beta Catenin