[Comparative serologic proteome analysis of Staphylococcus aureus and S. epidermidis exoproteins in prosthetic joint infections].
OBJECTIVE
Staphylococci such as Staphylococcus aureus and S. epidermidis are the most frequently pathogens associated to prosthesis joint infections (PJI), counting for 75% among the isolated bacteria. In this study, we identified PJI-related antigens using two-dimensional immunoblots of S. aureus and S. epidermidis exoproteins probed with serum samples from patients with confirmed PJIs. We further analysed by ELISA tests the response of patients to the identified proteins.


METHODS
Secreted proteins from Mu50 strain (S. aureus) and RP62A strain (S. epidermidis) were separated by 2D gel electrophoresis and analyzed by western blot with serum samples from patients with confirmed S. aureus and S. epidermidis PJIs. Recombinant proteins corresponding to the identified proteins were expressed and screened with an in-house ELISA to evaluate their interest for the diagnosis of S. aureus and S. epidermidis PJIs.


RESULTS
Fifty-two antigenic exoproteins were identified: 42 belonging to Mu50 strain, and 10 to RP62A strain. Twenty-two proteins were identified as S. aureus specific. Among these proteins, five were most frequently recognized by patients with S. aureus PJI.


CONCLUSIONS
Our results showed that few exoproteins were antigenic by RP62A strain compared to Mu50 strain. We identified five antigenic and S. aureus specific proteins, which may contribute to diagnosis, prevention and treatment of these infections.
Nuyttens
H
H
InGen Biosciences SA, 17, avenue du Parc, 91380 Chilly-Mazarin, France. hnuyttens@ingenbiosciences.com
Thomas
D
D
Rogé
J
J
Mignon
K
K
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000942:Antigens, Bacterial / Q000097:blood*; D001170:Arthritis, Infectious / Q000097:blood* / Q000382:microbiology; D001426:Bacterial Proteins / Q000097:blood*; D001782:Blood Donors; D015153:Blotting, Western; D015180:Electrophoresis, Gel, Two-Dimensional; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D016459:Prosthesis-Related Infections / Q000097:blood* / Q000382:microbiology; D020543:Proteome; D013045:Species Specificity; D013203:Staphylococcal Infections / Q000097:blood* / Q000382:microbiology; D013211:Staphylococcus aureus / Q000378:metabolism*; D013212:Staphylococcus epidermidis / Q000378:metabolism*
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D020543:Proteome