RNAi screening for host factors involved in Vaccinia virus infection using Drosophila cells.
Viral pathogens represent a significant public health threat; not only can viruses cause natural epidemics of human disease, but their potential use in bioterrorism is also a concern. A better understanding of the cellular factors that impact infection would facilitate the development of much-needed therapeutics. Recent advances in RNA interference (RNAi) technology coupled with complete genome sequencing of several organisms has led to the optimization of genome-wide, cell-based loss-of-function screens. Drosophila cells are particularly amenable to genome-scale screens because of the ease and efficiency of RNAi in this system (1). Importantly, a wide variety of viruses can infect Drosophila cells, including a number of mammalian viruses of medical and agricultural importance (2,3,4). Previous RNAi screens in Drosophila have identified host factors that are required for various steps in virus infection including entry, translation and RNA replication (5). Moreover, many of the cellular factors required for viral replication in Drosophila cell culture are also limiting in human cells infected with these viruses (4,6,7,8, 9). Therefore, the identification of host factors co-opted during viral infection presents novel targets for antiviral therapeutics. Here we present a generalized protocol for a high-throughput RNAi screen to identify cellular factors involved in viral infection, using vaccinia virus as an example.
Moser
Theresa S
TS
Department of Microbiology, Penn Genome Frontiers Institute, University of Pennsylvania-School of Medicine, USA.
Sabin
Leah R
LR
Cherry
Sara
S
D000818:Animals; D002478:Cells, Cultured; D004330:Drosophila / Q000235:genetics* / Q000821:virology*; D034622:RNA Interference; D014615:Vaccinia / Q000235:genetics*; D014616:Vaccinia virus / Q000472:pathogenicity*