title: A liquid chromatography-tandem mass spectrometry method for the quantification of PAC-1 in rat plasma.
abstract: A sensitive and specific liquid chromatography coupled with tandem mass spectrometry (LC/MS/MS) method was developed and validated for the determination of PAC-1 in rat plasma. After extraction with ethyl acetate, the chromatographic separation was carried out on an ACQUITY UPLC™ BEH C(18) column, with acetonitrile and water (39:61 (v/v) both containing 0.1% formic acid) as mobile phase at a flow rate of 0.20 mL/min. The detection was performed on a triple quadrupole tandem mass spectrometer by multiple reaction monitoring (MRM) mode via electrospray ionization (ESI) source. The calibration curve was linear over the range of 10-1500 ng/mL (r>0.99). The LOQ was evaluated to be 0.3 ng/mL. The method described herein is sensitive, selective and faster than other existing method, and was successfully applied to the pharmacokinetic study and gender difference investigation of PAC-1 after oral administration in rats.
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Wenhua Road 103, Shenyang 110016, PR China.
authors.forename: Li-Na
authors.initials: LN
authors.lastname: Fang
authors.forename: Xiao-Hui
authors.initials: XH
authors.lastname: Chen
authors.forename: Qiu-Di
authors.initials: QD
authors.lastname: Wang
authors.forename: Di
authors.initials: D
authors.lastname: Zhang
authors.forename: Juan-Juan
authors.initials: JJ
authors.lastname: Zhao
authors.forename: Zhi-Min
authors.initials: ZM
authors.lastname: Long
authors.forename: Ping
authors.initials: P
authors.lastname: Gong
authors.forename: Kai-Shun
authors.initials: KS
authors.lastname: Bi
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002138:Calibration; D020169:Caspases / Q000097:blood*; D002623:Chemistry Techniques, Analytical; D002626:Chemistry, Pharmaceutical / Q000379:methods; D002853:Chromatography, Liquid / Q000379:methods*; D005260:Female; D006835:Hydrazones / Q000032:analysis* / Q000737:chemistry; D007700:Kinetics; D008297:Male; D008956:Models, Chemical; D010879:Piperazines / Q000032:analysis* / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: C514579:(4-benzylpiperazin-1-yl)acetic acid (3-allyl-2-hydroxybenzylidene)hydrazine; D006835:Hydrazones; D010879:Piperazines; D020169:Caspases