title: Isolation of a novel bacteriophage specific for the periodontal pathogen Fusobacterium nucleatum.
abstract: Fusobacterium nucleatum is a periodontal pathogen that has been directly associated with the development and progression of periodontal disease, a widespread pathology that affects the support tissues of the tooth. We isolated a new bacteriophage (FnpΦ02) that specifically infects this bacterium. Transmission electron microscopy showed that the virion is composed of an icosahedral head and a segmented tail. The size of the phage genome was estimated to be approximately 59 kbp of double-stranded DNA. The morphological features and the genetic characteristics suggest that FnpΦ02 is part of the Siphoviridae family. Using one-step growth and adsorption experiments, the latent period, burst size, and adsorption rate were estimated to be 15 h, 100 infectious units per cell, and 7.5 × 10⁻¹⁰ ml min⁻¹, respectively. A small fragment of phage DNA was cloned and sequenced, showing 93% nucleotide identity with the phage PA6 of Propionibacterium acnes and amino acid identity with fragments of two proteins (Gp3 and Gp4) of this phage. To our knowledge, FnpΦ02 is the first phage described to infect Fusobacterium nucleatum and provides the base for future exploration of phages in the control of periodontal disease.
authors.affiliation: Laboratorio de Microbiologia y Biotecnologia Oral, Departamento de Ciencias Biologicas, Facultad de Ciencias Biologicas, Universidad Andres Bello, Echaurren 237, Santiago, Chile.
authors.forename: Pamela
authors.initials: P
authors.lastname: Machuca
authors.forename: Leslie
authors.initials: L
authors.lastname: Daille
authors.forename: Enrique
authors.initials: E
authors.lastname: Vinés
authors.forename: Liliana
authors.initials: L
authors.lastname: Berrocal
authors.forename: Mauricio
authors.initials: M
authors.lastname: Bittner
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D005674:Fusobacterium Infections / Q000821:virology*; D016967:Fusobacterium nucleatum / Q000821:virology*; D006801:Humans; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D010510:Periodontal Diseases / Q000382:microbiology* / Q000821:virology; D017906:Siphoviridae / Q000235:genetics / Q000302:isolation & purification* / Q000648:ultrastructure
chemical_list: D004269:DNA, Bacterial