title: Permanent gold nanoparticle coatings on polyelectrolyte multilayer modified capillaries for open-tubular capillary electrochromatography.
abstract: This paper reports on a new strategy to coat fused silica capillaries through ionic adsorption of gold nanoparticles (AuNPs) on a polyelectrolyte multilayer (PEM) modified capillary wall. The coating was constructed in situ by alternating rinses with positively charged poly(diallydimethylammonium chloride), negatively charged poly(sodium-4-styrenesulfonate), and positively charged AuNPs. After self-assembly of n-octadecanethiol onto the surface of AuNPs, the modified capillary was investigated as a new medium for the separation of neutral analytes and proteins in open-tubular capillary electrochromatography (OT-CEC). The surface coverage of the capillary wall was increased using the high density of AuNPs which were dynamically capped with 4-dimethylaminopyridine (DMAP). The chromatographic performance of the column coated with positively charged AuNPs was remarkably improved compared with a column modified with negatively charged AuNPs. The coating was robust over more than 810 runs in this study and also showed high stability against 0.01 M NaOH, 0.01 M HCl, and electrolyte concentrations up to 70 mM. The run-to-run, day-to-day, and capillary-to-capillary reproducibilities of electroosmotic flow were satisfying with relative standard deviation values of less than 1% in all cases. The AuNP-coated PEM modified capillary column not only showed good performance for neutral analytes but also was suitable for the analysis of both basic and acidic proteins.
authors.affiliation: Jiangsu Key Laboratory of Environmental Materials and Environmental Engineering, College of Chemistry and Chemical Engineering, Yangzhou University, Siwangting Road 180, Yangzhou 225002, Jiangsu, China. quqishu@gmail.com
authors.forename: Qishu
authors.initials: Q
authors.lastname: Qu
authors.forename: Dengping
authors.initials: D
authors.lastname: Liu
authors.forename: Debby
authors.initials: D
authors.lastname: Mangelings
authors.forename: Chun
authors.initials: C
authors.lastname: Yang
authors.forename: Xiaoya
authors.initials: X
authors.lastname: Hu
mesh_terms: D000818:Animals; D053801:Capillary Electrochromatography / Q000295:instrumentation / Q000379:methods*; D002645:Chickens; D004527:Egg Proteins / Q000737:chemistry / Q000302:isolation & purification; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D000432:Methanol / Q000737:chemistry; D018625:Microscopy, Atomic Force; D046529:Microscopy, Electron, Transmission; D011095:Polyethylenes / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D015203:Reproducibility of Results; D012822:Silicon Dioxide; D013451:Sulfonic Acids / Q000737:chemistry
chemical_list: D004527:Egg Proteins; D011095:Polyethylenes; D011108:Polymers; D000644:Quaternary Ammonium Compounds; D013451:Sulfonic Acids; C041004:poly-N,N-dimethyl-N,N-diallylammonium chloride; D006046:Gold; D012822:Silicon Dioxide; D000432:Methanol; C077114:styrenesulfonic acid polymer