Construction of small-insert and large-insert metagenomic libraries.
The vast majority of the Earth's biological diversity is hidden in uncultured and yet uncharacterized microbial genomes. The construction of metagenomic libraries is a cultivation-independent molecular approach to assess this unexplored genetic reservoir. In the last few years, a high number of novel biocatalysts have been identified by function-based or sequence-based screening of metagenomic libraries. Here, we describe detailed protocols for the construction of metagenomic small-insert and large-insert libraries in plasmids and fosmids, respectively, from environmental DNA.
Simon
Carola
C
Institute of Microbiology and Genetics, Georg August University Göttingen, Göttingen, Germany.
Daniel
Rolf
R
D044822:Biodiversity; D004247:DNA / Q000032:analysis / Q000235:genetics; D015723:Gene Library; D016680:Genome, Bacterial; D054892:Metagenome / Q000235:genetics*; D056186:Metagenomics / Q000379:methods*
D004247:DNA