[Curcumin inhibits hypoxia-induced proliferation of MRC-5 and collagen I synthesis].
OBJECTIVE
To investigate the effect of curcumin on MRC-5 in human embryo fibroblast (HEF) under hypoxia and possible underlying mechanism.


METHODS
The MRC-5 were cultured in vivo under 5 different conditions, including normal control, SB203580 (10 micromol/L), LY-294002 (10 micromol/L) and curcumin (5,10 and 20 micromol/L, respectively). The proliferation of MRC-5 was assessed by MTT assay and the expressions of pro-collal, p-p38 and p-AKT were detected by western blot.


RESULTS
Compared with the nomoxia control, hypoxia enhanced the proliferation of MRC-5 and the expressions of pro-collal, p-p38 and p-AKT (P < 0.05). Curcumin inhibited the hypoxia-induced proliferation of MRC and the expression of pro-collal and p-p38 (P < 0.05), but not the expression of p-AKT (P > 0.05).


CONCLUSIONS
Hypoxia might enhance the proliferation of MRC-5 cells and the expression of collagen I through PI3K/AKT and p38MAPK. Curcumin blocks the hypoxia influence through is impact on p38MAPK.
He
Zheng-Ping
ZP
Department of Respiratory, West China Hospital, Sichuan University, Chengdu 610041, China.
Xie
Min
M
D015687:Cell Hypoxia / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects*; D024042:Collagen Type I / Q000096:biosynthesis*; D003474:Curcumin / Q000494:pharmacology*; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D008168:Lung / Q000166:cytology*; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
D024042:Collagen Type I; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; D048051:p38 Mitogen-Activated Protein Kinases; D003474:Curcumin