Single-cell bacteria growth monitoring by automated DEP-facilitated image analysis.
Growth monitoring is the method of choice in many assays measuring the presence or properties of pathogens, e.g. in diagnostics and food quality. Established methods, relying on culturing large numbers of bacteria, are rather time-consuming, while in healthcare time often is crucial. Several new approaches have been published, mostly aiming at assaying growth or other properties of a small number of bacteria. However, no method so far readily achieves single-cell resolution with a convenient and easy to handle setup that offers the possibility for automation and high throughput. We demonstrate these benefits in this study by employing dielectrophoretic capturing of bacteria in microfluidic electrode structures, optical detection and automated bacteria identification and counting with image analysis algorithms. For a proof-of-principle experiment we chose an antibiotic susceptibility test with Escherichia coli and polymyxin B. Growth monitoring is demonstrated on single cells and the impact of the antibiotic on the growth rate is shown. The minimum inhibitory concentration as a standard diagnostic parameter is derived from a dose-response plot. This report is the basis for further integration of image analysis code into device control. Ultimately, an automated and parallelized setup may be created, using an optical microscanner and many of the electrode structures simultaneously. Sufficient data for a sound statistical evaluation and a confirmation of the initial findings can then be generated in a single experiment.
Peitz
Ingmar
I
Philips Research Europe, High Tech Campus, Prof. Holstlaan 4, 5656AE, Eindhoven, The Netherlands. IngmarPeitz@web.de
van Leeuwen
Rien
R
D000465:Algorithms; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001331:Automation; D004566:Electrodes; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development*; D008826:Microbial Sensitivity Tests; D044085:Microfluidics; D011112:Polymyxin B / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D011112:Polymyxin B