Neuron-specific expression of atp6v0c2 in zebrafish CNS.
Vacuolar ATPase (V-ATPase) is a multi-subunit enzyme that plays an important role in the acidification of a variety of intracellular compartments. ATP6V0C is subunit c of the V(0) domain that forms the proteolipid pore of the enzyme. In the present study, we investigated the neuron-specific expression of atp6v0c2, a novel isoform of the V-ATPase c-subunit, during the development of the zebrafish CNS. Zebrafish atp6v0c2 was isolated from a genome-wide analysis of the zebrafish mib(ta52b) mutant designed to identify genes differentially regulated by Notch signaling. Whole-mount in situ hybridization revealed that atp6v0c2 is expressed in a subset of CNS neurons beginning several hours after the emergence of post-mitotic neurons. The ATP6V0C2 protein is co-localized with the presynaptic vesicle marker, SV2, suggesting that it is involved in neurotransmitter storage and/or secretion in neurons. In addition, the loss-of-function experiment suggests that ATP6V0C2 is involved in the control of neuronal excitability.
Chung
Ah-Young
AY
School of Life Science and Biotechnology, Kyungpook National University, Daegu, Korea.
Kim
Myoung-Jin
MJ
Kim
Dohyun
D
Bang
Sangsu
S
Hwang
Sun Wook
SW
Lim
Chae Seung
CS
Lee
Sanggyu
S
Park
Hae-Chul
HC
Huh
Tae-Lin
TL
D000595:Amino Acid Sequence; D000818:Animals; D002490:Central Nervous System / Q000033:anatomy & histology / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism*; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism*; D051880:Receptors, Notch / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D015398:Signal Transduction / Q000502:physiology; D025262:Vacuolar Proton-Translocating ATPases / Q000235:genetics / Q000378:metabolism*; D015027:Zebrafish / Q000033:anatomy & histology / Q000235:genetics / Q000378:metabolism; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism*
D020033:Protein Isoforms; D021122:Protein Subunits; D051880:Receptors, Notch; D029961:Zebrafish Proteins; D025262:Vacuolar Proton-Translocating ATPases