title: Endocannabinoid signaling directs differentiation of trophoblast cell lineages and placentation.
abstract: In most mammals, placentation is critical for fetal development and pregnancy success. Exposure to marijuana during pregnancy has adverse effects, but whether the placenta is a target of cannabinoid/endocannabinoid signaling is not known. Using mice as a model system, we found that the endocannabinoid system is present in the ectoplacental cone and spongiotrophoblast cells. We also observed that aberrant endocannabinoid signaling confers premature trophoblast stem cell differentiation, and defective trophoblast development and invasion. These defects are reflected in retarded fetal development and compromised pregnancy outcome. Because the endocannabinoid system is conserved in mice and humans, our study suggests that endocannabinoid signaling is critical to placentation and pregnancy success in humans and implicates its potential significance in stem cell biology.
authors.affiliation: Division of Reproductive Sciences, Perinatal Institute, Cincinnati Children's Hospital Medical Center, Cincinnati, OH 45229, USA.
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Sun
authors.forename: Huirong
authors.initials: H
authors.lastname: Xie
authors.forename: Jie
authors.initials: J
authors.lastname: Yang
authors.forename: Haibin
authors.initials: H
authors.lastname: Wang
authors.forename: Heather B
authors.initials: HB
authors.lastname: Bradshaw
authors.forename: Sudhansu K
authors.initials: SK
authors.lastname: Dey
mesh_terms: D000818:Animals; D063385:Cannabinoid Receptor Modulators / Q000235:genetics / Q000378:metabolism*; D002454:Cell Differentiation; D019070:Cell Lineage; D049109:Cell Proliferation; D063388:Endocannabinoids; D005260:Female; D005313:Fetal Death / Q000235:genetics; D051379:Mice; D018345:Mice, Knockout; D010929:Placentation / Q000235:genetics / Q000502:physiology*; D011247:Pregnancy; D043884:Receptor, Cannabinoid, CB1 / Q000235:genetics; D015398:Signal Transduction; D014327:Trophoblasts / Q000166:cytology* / Q000378:metabolism
chemical_list: D063385:Cannabinoid Receptor Modulators; D063388:Endocannabinoids; D043884:Receptor, Cannabinoid, CB1