AFM characterization of spin-coated multilayered dry lipid films prepared from aqueous vesicle suspensions.
We present a detailed AFM study on multilayered dry lipid films prepared from aqueous vesicle suspensions. Different preparation techniques were applied in order to optimize the preparation of homogeneous lipid films of various film thicknesses. Suspensions of preformed DOPC/DPPC vesicles were adsorbed onto indium tin oxide-coated glass coverslips, a substrate also commonly employed for the formation of giant liposomes. We found that the homogeneity of the lipid films could substantially be improved when applying a spin-coating step during the film preparation. These films were much more homogeneous than those prepared by conventional drop-casting and in addition the film thickness could be controlled. When using a combination of vesicle adsorption and spin-coating the quality and thickness of the films depended crucially on the lipid concentration of the vesicle suspension, the adsorption temperature and the adsorption time. For lipid films prepared by direct spin-coating the lipid concentration and the applied spin-coating sequence were critical parameters for the quality and thickness of the deposited lipid films.
Krapf
Lisa
L
Lehrstuhl für Experimentalphysik IV and Research Centre for BioMacromolecules, Universität Bayreuth, Universtitätsstrasse 30, D-95440 Bayreuth, Germany. krapf@bmo.uni-luebeck.de
Dezi
Manuela
M
Reichstein
Werner
W
Köhler
Jürgen
J
Oellerich
Silke
S
D008055:Lipids / Q000737:chemistry*; D008422:Materials Testing / Q000379:methods*; D018625:Microscopy, Atomic Force; D010713:Phosphatidylcholines / Q000138:chemical synthesis* / Q000737:chemistry; D012996:Solutions; D013535:Suspensions; D017971:Tin Compounds / Q000737:chemistry; D053835:Unilamellar Liposomes / Q000737:chemistry*; D014867:Water / Q000737:chemistry*
D008055:Lipids; D010713:Phosphatidylcholines; D012996:Solutions; D013535:Suspensions; D017971:Tin Compounds; D053835:Unilamellar Liposomes; D014867:Water; C109984:indium tin oxide; C017251:1,2-oleoylphosphatidylcholine