Spermatotoxicity, biochemical changes and histological alteration induced by gossypol in testicular and hepatic tissues of male rats.
Gossypol acetic acid (GAA) displays anti-fertility and antioxidant behavior. The efficacies of different doses of gossypol acetic acid were investigated in male albino rats. Rats were allocated into four groups: control group and three GAA-treated groups (2-4), that were injected with GAA (5, 10, 20mg/kg BW, respectively), through inrtaperitonial injection. Treatment of GAA was found to elicit a significant decrease in sperm counting, sperm motility, serum levels of testosterone, luteinizing hormone and follicle-stimulating hormone, whereas, the activities of testicular 17β-hydroxysteroid dehydrogenase and 17-ketosteroid reductase were increased. The activities of serum transaminases and alkaline phosphatase and hepatic glutathione peroxidase; glutathione reductase, superoxide dismutase and glutathione S-transferase and the level of hepatic glutathione were elevated. While, the lipid peroxidation end product; malondialdehyde, nitric oxide, and lipid profile and the activity of hepatic cytochrome P450 were decreased in GAA-treated rats. The histological analysis of liver and testicular tissues showed sever hepatocyte damage in addition to abnormal localization of hepatocytic nuclei. Also, the testicular pathology of GAA-treated rats showed depressed spermatogensis, sertoli cell toxicity and degeneration of seminiferous tubules.
El-Sharaky
A S
AS
Department of Biochemistry, Faculty of Science, Alexandria University, Alexandria, Egypt. aselsharaky@ymail.com
Newairy
A A
AA
Elguindy
N M
NM
Elwafa
A A
AA
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D056486:Chemical and Drug Induced Liver Injury / Q000201:enzymology / Q000378:metabolism / Q000473:pathology*; D005640:Follicle Stimulating Hormone / Q000378:metabolism; D006072:Gossypol / Q000494:pharmacology / Q000633:toxicity*; D022781:Hepatocytes / Q000187:drug effects / Q000473:pathology; D007248:Infertility, Male / Q000139:chemically induced / Q000473:pathology; D050356:Lipid Metabolism / Q000187:drug effects; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000201:enzymology / Q000473:pathology*; D007986:Luteinizing Hormone / Q000378:metabolism; D008297:Male; D016877:Oxidants / Q000633:toxicity; D051381:Rats; D017207:Rats, Sprague-Dawley; D012661:Semen / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects*; D013733:Testicular Diseases / Q000139:chemically induced* / Q000378:metabolism / Q000473:pathology*; D013737:Testis / Q000473:pathology*; D013739:Testosterone / Q000378:metabolism
D000975:Antioxidants; D016877:Oxidants; D013739:Testosterone; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D006072:Gossypol