[Study on the relations between hepatitis B virus large surface protein (LHBs) and HBV YMDD mutation].
OBJECTIVE
To explore the Relations between hepatitis B virus large surface protein (LHBs) and HBV YMDD mutation, by detecting the hepatitis B virus large protein (LHBs) in Serum of patients with hepatitis B virus YMDD mutation.


METHODS
Selected patients after lamivudine treatment, to HBV YMDD mutation detection, then detected LHBs and HBV DNA in Serum of all patients. Serum HBV-DNA was quantitively detected by using real-time polymerase chain reaction (RT-PCR), the LHBS was detected by using enzyme linked immunosorbent assay (ELISA).


RESULTS
There was positive correlation between the A value of LHBs and HBV DNA level in 65 cases of mutation patients (r = 0.961). There was positive correlation between the A value of LHBs and HBV DNA level in 75 cases of non-mutation patients (r = 0.954).


CONCLUSIONS
There were positive correlation between the A value of LHBs and HBV DNA level in non-mutation patients and mutation patients, showed that LHBs can partially reflect the level of viral replication in patients, but not the impact of hepatitis B virus YMDD mutation.
Wang
Li-Jun
LJ
Shengsi County People's Hospital, Zhoushan 202450, China. henry_232@yahoo.com.cn
Sun
Yan
Y
Fu
Hai-Ping
HP
D000328:Adult; D000368:Aged; D020816:Amino Acid Motifs; D004259:DNA-Directed DNA Polymerase / Q000737:chemistry / Q000235:genetics*; D005260:Female; D006509:Hepatitis B / Q000097:blood* / Q000821:virology; D006515:Hepatitis B virus / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics / Q000502:physiology; D006801:Humans; D008297:Male; D008875:Middle Aged; D020125:Mutation, Missense; D014759:Viral Envelope Proteins / Q000097:blood*; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics*; D014779:Virus Replication; D055815:Young Adult
C096362:L protein, hepatitis B virus; D014759:Viral Envelope Proteins; D014764:Viral Proteins; D004259:DNA-Directed DNA Polymerase