PI3 kinase-dependent stimulation of platelet migration by stromal cell-derived factor 1 (SDF-1).
Platelets have been regarded as static cells that do not move once they adhere to a matrix. The present study explored, whether platelets are able to migrate. In contrast to the current opinion, we found that platelets were mobile, able to migrate over a surface, and transmigrate through a transwell membrane and endothelium toward a source of stromal cell-derived factor 1 (SDF-1). Platelet migration was stimulated by SDF-1, which led to the downstream activation and phosphorylation of Wiskott-Aldrich syndrome protein. SDF-1 signaling and subsequent platelet migration could be inhibited by CXCR4-receptor blocker AMD3100, pertussis toxin, inhibition of phosphoinositol 3-kinase (PI3 kinase) with LY294002 or wortmannin, and disruption of actin polymerization with cytochalasin B. The potential of platelets to migrate in an SDF-1-mediated fashion may redefine the role of platelets in the pathophysiology of vascular inflammation, subsequent atherosclerotic degeneration, and vascular regeneration.
Kraemer
Bjoern F
BF
Abteilung III, Kardiologie und Kreislauferkrankungen, Medizinische Klinik, Universitätsklinikum Tübingen, Otfried-Müller-Strasse 10, 72076, Tübingen, Germany.
Borst
Oliver
O
Gehring
Eva-Maria
EM
Schoenberger
Tanja
T
Urban
Benjamin
B
Ninci
Elena
E
Seizer
Peter
P
Schmidt
Christine
C
Bigalke
Boris
B
Koch
Miriam
M
Martinovic
Ivo
I
Daub
Karin
K
Merz
Tobias
T
Schwanitz
Laura
L
Stellos
Konstantinos
K
Fiesel
Fabienne
F
Schaller
Martin
M
Lang
Florian
F
Gawaz
Meinrad
M
Lindemann
Stephan
S
D000818:Animals; D001792:Blood Platelets / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology*; D001808:Blood Vessels / Q000187:drug effects / Q000473:pathology; D002465:Cell Movement / Q000187:drug effects*; D054377:Chemokine CXCL12 / Q000494:pharmacology*; D002633:Chemotaxis / Q000187:drug effects; D003094:Collagen / Q000494:pharmacology; D004727:Endothelium / Q000187:drug effects / Q000378:metabolism; D005340:Fibrinogen / Q000494:pharmacology; D019204:GTP-Binding Proteins / Q000378:metabolism; D006801:Humans; D007511:Ischemia / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D010766:Phosphorylation / Q000187:drug effects; D016062:Porosity / Q000187:drug effects; D019718:Receptors, CXCR4 / Q000378:metabolism; D051316:Wiskott-Aldrich Syndrome Protein / Q000378:metabolism
D054377:Chemokine CXCL12; D019718:Receptors, CXCR4; D051316:Wiskott-Aldrich Syndrome Protein; D005340:Fibrinogen; D003094:Collagen; D019869:Phosphatidylinositol 3-Kinases; D019204:GTP-Binding Proteins