Is a single nucleotide polymorphism a risk factor for lung cancer in the matrix metalloproteinase-2 promoter?
We aimed to investigate the association of polymorphism frequencies of MMP-2 C1306T and MMP-2 plasma enzyme activity in lung cancer patients. In this study 300 genomic DNA (200 lung cancer patients + 100 no lung cancer) were analyzed. Polymorphisms were determined by using polymerase chain reaction-restriction fragment length polymorphism (RFLP) and electrophoresis. Plasma MMP-2 enzyme activity levels were measured by using ELISA. Sex, asbestos expose and smoking might be risk factors for lung cancer. The frequencies of C1306T genotypes in controls CC 65%, CT 32%, TT 3% and in patients CC 61%, CT 37%, TT2 % were found. It was determined that CC genotype frequency increase significantly in patients and controls. Plasma MMP-2 enzyme activity levels were increased in lung cancer patients according to controls. Finally, we claimed that determining of MMP-2 enzyme level can be used as a marker in lung cancer.
Ayşegül
Bayramoglu
B
Faculty of Medicine, Department of Medical Biology, Eskisehir Osmangazi University, 26480 Eskisehir, Turkey. atbayramoglu@yahoo.com
Veysi
Gunes Hasan
GH
Muzaffer
Metintas
M
Irfan
Degirmenci
D
Azra
Akın
A
Hulyam
Kurt
K
D016022:Case-Control Studies; D005787:Gene Frequency / Q000235:genetics; D020022:Genetic Predisposition to Disease; D006801:Humans; D008175:Lung Neoplasms / Q000201:enzymology* / Q000235:genetics*; D020778:Matrix Metalloproteinase 2 / Q000097:blood / Q000235:genetics*; D009154:Mutation / Q000235:genetics; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012372:ROC Curve; D012307:Risk Factors
C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2