title: Metal binding to a zinc-finger peptide: a comparison between solution and the gas phase.
abstract: Solution-phase spectroscopy and mass spectrometry are used to probe interactions between divalent metal ions and a synthetic Cys(2)His(2) zinc-finger peptide (vCP1). Both methods provide the same order of binding affinity, zinc ≥ cobalt ≫ copper ≫ calcium. Collision-cross-section measurements show that both apo and holo forms are compact. This is corroborated by molecular-dynamics simulations.
authors.affiliation: School of Chemistry, University of Edinburgh, Edinburgh, UK.
authors.forename: Yana
authors.initials: Y
authors.lastname: Berezovskaya
authors.forename: Craig T
authors.initials: CT
authors.lastname: Armstrong
authors.forename: Aimee L
authors.initials: AL
authors.lastname: Boyle
authors.forename: Massimiliano
authors.initials: M
authors.lastname: Porrini
authors.forename: Derek N
authors.initials: DN
authors.lastname: Woolfson
authors.forename: Perdita E
authors.initials: PE
authors.lastname: Barran
mesh_terms: D002118:Calcium / Q000737:chemistry*; D003035:Cobalt / Q000737:chemistry*; D003300:Copper / Q000737:chemistry*; D003545:Cysteine / Q000737:chemistry; D005740:Gases / Q000737:chemistry; D006639:Histidine / Q000737:chemistry; D008958:Models, Molecular; D056004:Molecular Dynamics Simulation; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry*; D012996:Solutions; D015032:Zinc / Q000737:chemistry*; D016335:Zinc Fingers
chemical_list: D005740:Gases; D010455:Peptides; D012996:Solutions; D003035:Cobalt; D006639:Histidine; D003300:Copper; D015032:Zinc; D003545:Cysteine; D002118:Calcium