title: Antibody signatures defined by high-content peptide microarray analysis.
abstract: Circulating antibodies are highly selective binding reagents directed to a vast repertoire of antigens. Candidate antigens displayed as overlapping peptides on microarrays can be used to screen for recognition by serum antibodies from clinically well-defined patient populations. The methodology is robust and enables unbiased visualization of antigen-specific B-cell responses. Additionally, autoantibody signatures of diagnostic value could be detected using microarrays displaying thousands of human peptides.
authors.affiliation: JPT Peptide Technologies GmbH, Berlin, Germany.
authors.forename: Antonia
authors.initials: A
authors.lastname: Masch
authors.forename: Johannes
authors.initials: J
authors.lastname: Zerweck
authors.forename: Ulf
authors.initials: U
authors.lastname: Reimer
authors.forename: Holger
authors.initials: H
authors.lastname: Wenschuh
authors.forename: Mike
authors.initials: M
authors.lastname: Schutkowski
mesh_terms: D000818:Animals; D000906:Antibodies / Q000097:blood / Q000276:immunology*; D005189:False Positive Reactions; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007136:Immunoglobulins / Q000276:immunology; D010455:Peptides / Q000032:analysis* / Q000276:immunology; D040081:Protein Array Analysis / Q000379:methods*
chemical_list: D000906:Antibodies; D007136:Immunoglobulins; D010455:Peptides