Analysis of human synovial and bone marrow mesenchymal stem cells in relation to heat-inactivation of autologous and fetal bovine serums.
BACKGROUND
Though sera are essential for Mesenchymal stem cells (MSCs), the effect of heat-inactivation remains unknown. Autologous human serum is recommended for clinical use; however, it is unclear whether differentiation potentials are maintained. To examine whether heat-inactivation of serum affected the proliferation and whether autologous human serum influenced their multipotentiality.


METHODS
After whole blood collection, human synovium and bone marrow were harvested. Nucleated cells were expanded with autologous human serum and FBS.


RESULTS
Heat-inactivation of autologous human serum enhanced proliferation of synovial MSCs. Heat-inactivation of each types of serum didn't affect calcification of synovial MSCs. The induction of calcification increased ALP activity, with the exception of bone marrow MSCs with autologous human serum. For adipogenesis of synovial MSCs, the Oil Red-O positive colony forming efficiency with autologous human serum was similar to or less than that with FBS.


CONCLUSIONS
These clarified the processing of human autologous serum and the influence of different sera for differentiation of synovial and bone marrow MSCs.
Nimura
Akimoto
A
Section of Cartilage Regeneration, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan.
Muneta
Takeshi
T
Otabe
Koji
K
Koga
Hideyuki
H
Ju
Young-Jin
YJ
Mochizuki
Tomoyuki
T
Suzuki
Koji
K
Sekiya
Ichiro
I
D000328:Adult; D000818:Animals; D001798:Blood Proteins / Q000737:chemistry / Q000502:physiology*; D001854:Bone Marrow Cells / Q000166:cytology / Q000502:physiology*; D002417:Cattle; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation / Q000502:physiology; D049109:Cell Proliferation; D002478:Cells, Cultured; D005260:Female; D005333:Fetus; D006358:Hot Temperature / Q000009:adverse effects*; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology*; D039902:Multipotent Stem Cells / Q000166:cytology / Q000502:physiology; D013583:Synovial Membrane / Q000166:cytology / Q000502:physiology*; D055815:Young Adult
D001798:Blood Proteins