Combined peptides of human enterovirus 71 protect against virus infection in mice.
Human enterovirus 71 (EV71) is a cause of hand, foot and mouth disease (HMFD) in children under 6 years old, and could cause serious neurological complications in some patients. Numerous large outbreaks of EV71 caused HMFD have occurred recently in Asia, especially in China. The cross-reactivity of EV71 with human brain tissue was observed and the cross-reactivity inducing regions were identified in previously study, which suggested that there were two regions in structural proteins of virus should be avoided in the vaccine. Six peptides without cross-reactivity were selected and combined into three vaccine candidates and applied in further evaluation in neonatal mice. The Vac6 comprising the peptides of P(70-159), P(140-249), P(324-443) and P(746-876) of the structural proteins could provide effective protection on pups against virus infection, as shown in viral copies detection and histopathology examination. Immunohistochemical staining results indicated that Vac6 had no cross-reactivity with human brain tissues. Our results suggested that Vac6 could have potential clinical value against EV71 epidemics caused mainly by C4 strains in the mainland of China.
Liu
Jiang Ning
JN
Key Laboratory of Human Diseases Comparative Medicine, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences (CAMS) & Comparative Medicine Centre, Peking Union Medical Collage (PUMC), Beijing 100021, China. ljn zb03038@126.com
Wang
Wei
W
Duo
Jian Ying
JY
Hao
Yi
Y
Ma
Chun Mei
CM
Li
Wan Bo
WB
Lin
Shu Zhu
SZ
Gao
Xue Zhong
XZ
Liu
Xiao Lin
XL
Xu
Yan Feng
YF
Xu
Wen-Bo
WB
Qin
Chuan
C
Zhang
Lian Feng
LF
D000818:Animals; D000831:Animals, Newborn; D057134:Antibodies, Neutralizing / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood; D001921:Brain / Q000276:immunology / Q000821:virology; D003429:Cross Reactions; D004195:Disease Models, Animal; D029821:Enterovirus A, Human / Q000276:immunology*; D004769:Enterovirus Infections / Q000276:immunology / Q000517:prevention & control*; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009500:Neutralization Tests; D010455:Peptides / Q000276:immunology*; D015678:Viral Structural Proteins / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology*
D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D010455:Peptides; D015678:Viral Structural Proteins; D014765:Viral Vaccines