title: [Flow cytometric analysis for detecting mitochondrial permeability transition pore opening].
abstract: OBJECTIVE
  To introduce a new method for detecting mitochondrial permeability transition pore (PTP) opening with flow cytometry using the resveratrol-inducing PTP opening model.
  
  
  METHODS
  Mitochondria were isolated from rat livers and selectively labeled with nonyl acridine orange. The mitochondrial membrane potential was detected using flow cytometry with TMRE (tetramethylrhodamine, ethyl ester) labeling. PTP opening induced by resveratrol was represented by the changes of mitochondrial side-scattering (SSC) detected by flow cytometry.
  
  
  RESULTS
  Flow cytometry was capable of defining the purity of the mitochondria isolated. The fluorescence intensities and SSC of the mitochondria were decreased after resveratrol treatment, indicating that resveratrol could induce PTP opening. Ciclosporin A inhibited resveratrol-induced PTP opening.
  
  
  CONCLUSIONS
  Flow cytometric analysis allows accurate and convenient detection of mitochondrial membrane potential, mitochondrial swelling and PTP opening.
authors.affiliation: Department of Histology and Embryology, Southern Medical University, Guangzhou 510515, China. yanfang@fimmu.com
authors.forename: Fang
authors.initials: F
authors.lastname: Yan
authors.forename: Xiao-dong
authors.initials: XD
authors.lastname: Ma
authors.forename: Xue-mei
authors.initials: XM
authors.lastname: Tian
mesh_terms: D000818:Animals; D017209:Apoptosis; D005434:Flow Cytometry; D053078:Membrane Potential, Mitochondrial / Q000235:genetics*; D008930:Mitochondria, Liver / Q000378:metabolism; D033681:Mitochondrial Membrane Transport Proteins / Q000378:metabolism*; D000083162:Mitochondrial Permeability Transition Pore; D051381:Rats; D012235:Rhodamines
chemical_list: D033681:Mitochondrial Membrane Transport Proteins; D000083162:Mitochondrial Permeability Transition Pore; D012235:Rhodamines; C005358:tetramethylrhodamine