title: Experimental design as a tool to evaluate chlorogenic and caffeic acids extracted from Cecropia glaziovii Sneth.
abstract: The effects of different parameters, including ethanol concentration, time of drug:solvent contact, temperature and the presence of a preservative, on chlorogenic acid (CGA) and caffeic acid (CFA) yields in Cecropia glaziovii Sneth extracts were investigated using an experimental design. In order to quantify the phenolic acids in these extracts a high-performance liquid chromatography-diode array detection (HPLC-DAD) method was developed and validated. Extracts with 80% ethanol presented a higher CGA content, but low amounts of CFA. Extracts with 20% ethanol showed a higher CFA concentration, but a sharp reduction in CGA extraction yield. The presence of a preservative, under the same extraction conditions, resulted in a slight difference or no difference in the CGA and CFA extraction yields. When the temperature was controlled at refrigerator or room temperature, a slight alteration in the concentrations of the phenolics studied was observed. The present approach can be applied in order to determine the optimum conditions for the preparation of C. glaziovii Sneth extracts based on CGA or CFA extraction yield as a chemical marker.
authors.affiliation: Laboratório de Farmacotécnica, Departamento de Ciências Farmacêuticas, Centro de Ciências da Saúde, Universidade Federal de Santa Catarina (UFSC), Florianópolis, Brazil.
authors.forename: Daniela P
authors.initials: DP
authors.lastname: Arend
authors.forename: Talitha Caldas
authors.initials: TC
authors.lastname: dos Santos
authors.forename: Diva
authors.initials: D
authors.lastname: Sonaglio
authors.forename: Ana Lúcia Gomes
authors.initials: AL
authors.lastname: Dos Santos
authors.forename: Flávio H
authors.initials: FH
authors.lastname: Reginatto
authors.forename: Angela M
authors.initials: AM
authors.lastname: de Campos
mesh_terms: D002109:Caffeic Acids / Q000737:chemistry*; D002138:Calibration; D035702:Cecropia Plant / Q000378:metabolism*; D002623:Chemistry Techniques, Analytical; D002626:Chemistry, Pharmaceutical / Q000379:methods; D002726:Chlorogenic Acid / Q000737:chemistry*; D002845:Chromatography / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D000431:Ethanol / Q000737:chemistry; D010316:Particle Size; D015203:Reproducibility of Results; D013696:Temperature; D013997:Time Factors; D014466:Ultraviolet Rays
chemical_list: D002109:Caffeic Acids; D002726:Chlorogenic Acid; D000431:Ethanol