title: Prognostic significance of nestin expression in resected non-small cell lung cancer.
abstract: BACKGROUND
  Nestin is a class 6 intermediate filament protein expressed in stem/progenitor cells during CNS development. Nestin expression has been detected in many kinds of tumors and was reported in a recent small-scale study in non-small cell lung cancer (NSCLC). We investigated the relationships between nestin expression and clinicopathologic parameters and determined its prognostic significance concerning survival in patients with resected NSCLC.
  
  
  METHODS
  Nestin expression in tumor cells was studied immunohistochemically in 171 consecutive patients with NSCLC, and associations with clinicopathologic parameters were evaluated. Kaplan-Meier survival analysis and Cox proportional hazards models were used to estimate the effect of nestin expression on survival.
  
  
  RESULTS
  Nestin expression was observed in tumor cell samples in 27 of the 171 patients with NSCLC (15.8%). Nestin had only cytoplasmic expression. Clinicopathologically, nestin expression was significantly associated with squamous cell carcinoma (P = .001), poorer differentiation (P = .007), lymph node metastasis (P = .008), intratumoral vascular invasion (P = .003), intratumoral lymphatic invasion (P = .008), pleural invasion (P = .039), and poorer prognosis (P < .001). Multivariable analysis confirmed that nestin expression increased the hazard of death after adjusting for other clinicopathologic factors (hazard ratio, 2.75; 95% CI, 1.39-5.46).
  
  
  CONCLUSIONS
  The present study suggests that nestin expression is a prognostic indicator of poorer survival probability for patients with resected NSCLC and may be used as a potential marker for select patients who should receive adjuvant chemotherapy.
authors.affiliation: Department of Respiratory Medicine, Kitasato University, Kanagawa, Japan.
authors.forename: Shinichiro
authors.initials: S
authors.lastname: Ryuge
authors.affiliation: Department of Molecular Diagnostics, School of Allied Health Sciences, Kitasato University, Kanagawa, Japan. Electronic address: yuichi@med.kitasato-u.ac.jp.
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Sato
authors.affiliation: Kitasato Clinical Research Center, Kitasato University, Kanagawa, Japan.
authors.forename: Guo Qin
authors.initials: GQ
authors.lastname: Wang
authors.affiliation: Department of Cellular and Histo-Pathology, Graduate School of Medical Sciences, Kitasato University, Kanagawa, Japan.
authors.forename: Toshihide
authors.initials: T
authors.lastname: Matsumoto
authors.affiliation: Department of Pathology, Kitasato University, Kanagawa, Japan.
authors.forename: Shi Xu
authors.initials: SX
authors.lastname: Jiang
authors.affiliation: Department of Respiratory Medicine, Kitasato University, Kanagawa, Japan.
authors.forename: Ken
authors.initials: K
authors.lastname: Katono
authors.affiliation: Department of Thoracic Surgery, National Hospital Organization Sagamihara National Hospital, Kanagawa, Japan.
authors.forename: Hayato
authors.initials: H
authors.lastname: Inoue
authors.affiliation: Department of Thoracic and Cardiovascular Surgery, School of Medicine, Kitasato University, Kanagawa, Japan.
authors.forename: Yukitoshi
authors.initials: Y
authors.lastname: Satoh
authors.affiliation: Department of Respiratory Medicine, Kitasato University, Kanagawa, Japan.
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Masuda
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002289:Carcinoma, Non-Small-Cell Lung / Q000378:metabolism* / Q000401:mortality / Q000601:surgery; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007150:Immunohistochemistry; D007381:Intermediate Filament Proteins / Q000096:biosynthesis*; D007564:Japan / Q000453:epidemiology; D008175:Lung Neoplasms / Q000378:metabolism* / Q000401:mortality / Q000601:surgery; D008297:Male; D008875:Middle Aged; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D064231:Nestin; D011013:Pneumonectomy / Q000401:mortality*; D011379:Prognosis; D012189:Retrospective Studies; D012307:Risk Factors
chemical_list: D007381:Intermediate Filament Proteins; C577484:NES protein, human; D009419:Nerve Tissue Proteins; D064231:Nestin