qPCR assays to quantify genes and gene expression associated with microbial perchlorate reduction.
Quantitative PCR (qPCR) assays targeting cld (developed in this work) and pcrA (previously described) were used to quantify these perchlorate-related genes in a perchlorate-reducing enrichment culture. Transcript copies were quantified in perchlorate-reducing Rhodocyclaceae strain JDS4. Oxygen and nitrate inhibited expression of cld and pcrA.
De Long
Susan K
SK
The University of Texas at Austin, Department of Civil, Architectural, and Environmental Engineering, 1 University Station C1786, Austin, TX 78712, United States. Susan.De_Long@colostate.edu
Kinney
Kerry A
KA
Kirisits
Mary Jo
MJ
D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics; D004265:DNA Helicases / Q000096:biosynthesis / Q000235:genetics; D020869:Gene Expression Profiling / Q000379:methods*; D009566:Nitrates / Q000378:metabolism; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism; D010472:Perchlorates / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000379:methods*; D043362:Rhodocyclaceae / Q000235:genetics* / Q000378:metabolism*
D001426:Bacterial Proteins; D009566:Nitrates; D010472:Perchlorates; C084217:pcrA protein, Bacteria; C075969:rol protein, Bacteria; D004265:DNA Helicases; D010100:Oxygen; C494474:perchlorate