title: Genetic variation in monoamine oxidase A and analysis of association with behaviour traits in beef cattle.
abstract: Husbandry of beef cattle requires animals that do not behave aggressively or timidly. The enzyme monoamine oxidase A and the coding gene (MAOA) play an important role in the complex regulation of behaviour. The complete coding region and a part of the non-coding sequence of the bovine MAOA gene have been analysed in 20 German Angus and 20 German Simmental bulls and cows with the aim of detecting genetic variability. These two cattle breeds are known to differ regarding their behaviour during handling. Five single nucleotide polymorphisms (SNPs) were identified, three of which were found in the coding region of the gene (exons III and XV). One of the SNPs located in exon XV (NC_007331.3:g.80340C>T) was found to be a non-synonymous mutation. The minor allele frequency of this resulting amino acid substitution was significantly different between 543 German Angus and 417 German Simmental calves (0.39 and 0.49, respectively). The potential functional impact of this polymorphism has been tested by in silico analysis, as well as by association analysis using behaviour scores of the genotyped calves for three behaviour tests that assessed the animals' temperament during tethering, weighing or social separation. In silico analysis did not deliver consistent results arguing for or against a functional impact of the studied amino acid substitution on the function of the biological protein. No significant association was found between this MAOA polymorphism and the behaviour-related scores analysed in the study.
authors.affiliation: Department of Animal Breeding and Genetics, Justus-Liebig University of Giessen, Giessen, Germany.
authors.forename: G
authors.initials: G
authors.lastname: Lühken
authors.forename: K
authors.initials: K
authors.lastname: Glenske
authors.forename: H
authors.initials: H
authors.lastname: Brandt
authors.forename: G
authors.initials: G
authors.lastname: Erhardt
mesh_terms: D000822:Animal Husbandry; D000818:Animals; D001522:Behavior, Animal; D002417:Cattle / Q000235:genetics* / Q000502:physiology; D005260:Female; D005787:Gene Frequency; D008297:Male; D008995:Monoamine Oxidase / Q000235:genetics*; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA
chemical_list: D008995:Monoamine Oxidase