Effects of T-2 toxin on platelet-activating-factor-dependent phosphoinositide turnover in the bovine platelet.
The effect of T-2 toxin on phospholipid turnover in the bovine platelet was studied using washed platelets prelabelled with [1-(14)C]arachidonic acid. In the resting, non-stimulated platelet, T-2 toxin induced a significant increase in the incorporation of radioactivity into phosphatidic acid (PA). When the platelets were stimulated to aggregate with the agonist, platelet activating factor (PAF), a decrease in labelled phosphatidylinositol as well as a rise in radioactive PA and free fatty acid (FFA) were observed. In the presence of T-2 toxin, the PAF-dependent formation of the polyphosphoinositides and release of FFA were inhibited whereas PA accumulation was unaffected. These results may be of importance in understanding the mode of action of T-2 toxin at the level of membrane phospholipid turnover.
Bondy
G S
GS
Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada N1G 2W1.
Holub
B J
BJ
Gentry
P A
PA