title: Use of anchorchip-time-of-flight spectrometry technology to screen tumor biomarker proteins in serum for small cell lung cancer.
abstract: BACKGROUND
  The purpose of this study is to discover potential biomarkers in serum for the detection of small cell lung cancer (SCLC).
  
  
  METHODS
  74 serum samples including 30 from SCLC patients and 44 from healthy controls were analyzed using ClinProt system combined with matrix-assisted laser desorption/ionization time-of-flight masss spectrometry (MALDI-TOF-MS). ClinProt software and genetic algorithm analysis selected a panel of serum markers that most efficiently predicted which patients had SCLC.
  
  
  RESULTS
  The diagnostic pattern combined with 5 potential biomarkers could differentiate SCLC patients from healthy persons, with a sensitivity of 90%, specificity of 97.73%. Remarkably, 88.89% of stage I/II patients were accurately assigned to SCLC.
  
  
  CONCLUSIONS
  Anchorchip-time-of-flight spectrometry technology will provide a highly accurate approach for discovering new biomarkers for the detection of SCLC.
authors.affiliation: Department of Respiratory Medicine, Second Affiliated Hospital of Medical School, Xi'an Jiaotong University, N-710000 Xi'an, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Du
authors.forename: Shuanying
authors.initials: S
authors.lastname: Yang
authors.forename: Xiuli
authors.initials: X
authors.lastname: Lin
authors.forename: Lina
authors.initials: L
authors.lastname: Bu
authors.forename: Yandong
authors.initials: Y
authors.lastname: Nan
authors.forename: Shufen
authors.initials: S
authors.lastname: Huo
authors.forename: Wenli
authors.initials: W
authors.lastname: Shang
mesh_terms: D000328:Adult; D000368:Aged; D000465:Algorithms; D014408:Biomarkers, Tumor / Q000097:blood*; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000097:blood* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000097:blood*; D009367:Neoplasm Staging; D011237:Predictive Value of Tests; D040901:Proteomics / Q000379:methods*; D012680:Sensitivity and Specificity; D055752:Small Cell Lung Carcinoma / Q000097:blood* / Q000473:pathology; D012984:Software; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins