title: Duration of exposure to environmental carcinogens affects DNA-adduct level in human lymphocytes.
abstract: OBJECTIVE
  An important issue in human biomonitoring is determining how exposure duration affects the kinetics of molecular biomarkers. In this study we compare the influence of exposure variables on DNA adducts.
  
  
  METHODS
  DNA adducts were analysed by 32P-postlabelling in lympho/monocytes of 677 Caucasian subjects.
  
  
  RESULTS
  After correction for other variables, DNA adducts increased depending on the length of occupational and smoke exposures. Higher DNA adducts were detected in workers with more than 14 years of exposure than in workers with shorter exposures (RR = 1.19, p = 0.049) and in smokers with more than 10 years of exposure than in smokers with shorter exposure (RR = 1.21, p <0.001).
  
  
  CONCLUSIONS
  Exposure length is the primary factor affecting DNA-adduct level in lympho/monocytes both in smokers and in occupationally exposed subjects.
authors.affiliation: Department of Health Sciences, University of Genoa, Italy.
authors.forename: Alberto
authors.initials: A
authors.lastname: Izzotti
authors.forename: Alessandra
authors.initials: A
authors.lastname: Pulliero
authors.forename: Riccardo
authors.initials: R
authors.lastname: Puntoni
authors.forename: Marco
authors.initials: M
authors.lastname: Peluso
authors.forename: Rosangela
authors.initials: R
authors.lastname: Filiberti
authors.forename: Armelle
authors.initials: A
authors.lastname: Munnia
authors.forename: Giorgio
authors.initials: G
authors.lastname: Assennato
authors.forename: Giovanni
authors.initials: G
authors.lastname: Ferri
authors.forename: Domenico Franco
authors.initials: DF
authors.lastname: Merlo
mesh_terms: D000328:Adult; D002274:Carcinogens, Environmental / Q000633:toxicity*; D003430:Cross-Sectional Studies; D018736:DNA Adducts / Q000097:blood*; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D016273:Occupational Exposure; D010761:Phosphorus Radioisotopes
chemical_list: D002274:Carcinogens, Environmental; D018736:DNA Adducts; D010761:Phosphorus Radioisotopes