title: Research resource: T-antigen transformation of pituitary cells captures three novel cell lines in the Pit-1 lineage.
abstract: We report the establishment of three distinct pituitary-derived murine cell lines generated by targeted T-antigen-induced transformation. The Pit1/0 line expresses pituitary-specific transcription factor-1 (Pit-1) but lacks expression of GH, prolactin (Prl), or TSH, and the Pit1/Prl line is selectively positive for Pit-1 and Prl. The third line, Pit1/Triple, expresses Pit-1 and all three of the Pit-1-dependent hormones: GH, Prl, and TSHβ/glycoprotein hormone α-subunit. The three corresponding transformation events appear to have captured pituitary cells representing: 1) an initial step in the Pit-1(+) lineage, 2) a cell line that corresponds to the differentiated lactotrope, and 3) a novel tri-hormone intermediate that may represent a pivotal step in Pit-1(+) cell lineage differentiation. The documented dependence of the tri-hormone expression in the Pit-1/Triple line on Pit-1 activity supports its potential role in the pathway of pituitary cell differentiation. The presence of a 123-kb human transgene encompassing the hGH locus (hGH/bacterial artificial chromosome) in two of these lines, Pit1/0 and Pit1/Prl, further expands their potential utility to the analysis of gene activation within the hGH gene cluster.
authors.affiliation: Room 560A Clinical Research Building, 415 Curie Boulevard, Philadelphia, Pennsylvania 19104, USA.
authors.forename: Daria
authors.initials: D
authors.lastname: Sizova
authors.forename: Yugong
authors.initials: Y
authors.lastname: Ho
authors.forename: Nancy E
authors.initials: NE
authors.lastname: Cooke
authors.forename: Stephen A
authors.initials: SA
authors.lastname: Liebhaber
mesh_terms: D000818:Animals; D000957:Antigens, Viral, Tumor / Q000378:metabolism*; D002461:Cell Line, Transformed / Q000166:cytology* / Q000473:pathology*; D019070:Cell Lineage; D002471:Cell Transformation, Neoplastic / Q000473:pathology*; D005455:Fluorescent Antibody Technique; D015972:Gene Expression Regulation, Neoplastic; D051379:Mice; D008822:Mice, Transgenic; D008954:Models, Biological; D010902:Pituitary Gland / Q000378:metabolism / Q000473:pathology*; D010911:Pituitary Neoplasms / Q000235:genetics / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D050817:Transcription Factor Pit-1 / Q000235:genetics / Q000378:metabolism*; D019076:Transgenes / Q000235:genetics
chemical_list: D000957:Antigens, Viral, Tumor; D012333:RNA, Messenger; D050817:Transcription Factor Pit-1