Rhizobium herbae sp. nov. and Rhizobium giardinii-related bacteria, minor microsymbionts of various wild legumes in China.
Seven Rhizobium strains associated with various legume species grown in different geographical regions of China were defined into four genomic groups related to Rhizobium giardinii, based upon ribosomal intergenic spacer RFLP, phylogenies of 16S rRNA and housekeeping (atpD, recA and glnII) genes, and DNA relatedness. Three strains in group I were classified as R. giardinii, as they showed high gene sequence similarities (>97 %) and DNA relatedness (64.3-67.5 %) to R. giardinii H152(T). Groups II, III and IV differed from all defined Rhizobium species based upon the consensus of all analyses. As group II contained two strains that originated from two distinct populations, we propose this group as a novel species, Rhizobium herbae sp. nov., with strain CCBAU 83011(T) ( = LMG 25718(T) = HAMBI 3117(T)) as the type strain.
Ren
Da Wei
DW
State Key Laboratories for Agrobiotechnology/College of Biological Sciences, China Agricultural University, Beijing 100193, PR China.
Wang
En Tao
ET
Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, 11340 México D. F., Mexico.|State Key Laboratories for Agrobiotechnology/College of Biological Sciences, China Agricultural University, Beijing 100193, PR China.
Chen
Wen Feng
WF
State Key Laboratories for Agrobiotechnology/College of Biological Sciences, China Agricultural University, Beijing 100193, PR China.
Sui
Xin Hua
XH
State Key Laboratories for Agrobiotechnology/College of Biological Sciences, China Agricultural University, Beijing 100193, PR China.
Zhang
Xiao Xia
XX
Agricultural Culture Collection of China, Institute of Agricultural Resource and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 10081, PR China.
Liu
Hong Can
HC
Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
Chen
Wen Xin
WX
State Key Laboratories for Agrobiotechnology/College of Biological Sciences, China Agricultural University, Beijing 100193, PR China.
D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D007887:Fabaceae / Q000382:microbiology* / Q000502:physiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012231:Rhizobium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D013559:Symbiosis
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S