title: Inhibitory components from the buds of clove (Syzygium aromaticum) on melanin formation in B16 melanoma cells.
abstract: In the course to find a new whitening agent, we evaluated the methanol extract from bud of clove (Syzygium aromaticum) on melanin formation in B16 melanoma cells. Eugenol and eugenol acetate were isolated as the active compounds and showed melanin inhibition of 60% and 40% in B16 melanoma cell with less cytotoxicity at the concentration of 100 and 200 μg/mL, respectively. Furthermore, an essential oil prepared from the bud of clove, which contain eugenol and eugenol acetate as dominant components, showed melanin inhibition of 50% and 80% in B16 melanoma cells at the concentration of 100 and 200 μg/mL, respectively.
authors.affiliation: Department of Agro-environmental Sciences, Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan.
authors.forename: Enos Tangke
authors.initials: ET
authors.lastname: Arung
authors.forename: Eri
authors.initials: E
authors.lastname: Matsubara
authors.forename: Irawan Wijaya
authors.initials: IW
authors.lastname: Kusuma
authors.forename: Edi
authors.initials: E
authors.lastname: Sukaton
authors.forename: Kuniyoshi
authors.initials: K
authors.lastname: Shimizu
authors.forename: Ryuichiro
authors.initials: R
authors.lastname: Kondo
mesh_terms: D000085:Acetates / Q000302:isolation & purification / Q000494:pharmacology; D000818:Animals; D045744:Cell Line, Tumor; D003879:Dermatologic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D005054:Eugenol / Q000302:isolation & purification / Q000494:pharmacology*; D035264:Flowers; D008543:Melanins / Q000037:antagonists & inhibitors*; D008546:Melanoma, Experimental / Q000378:metabolism*; D051379:Mice; D009822:Oils, Volatile / Q000737:chemistry / Q000494:pharmacology*; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D027842:Syzygium / Q000737:chemistry*
chemical_list: D000085:Acetates; D003879:Dermatologic Agents; D008543:Melanins; D009822:Oils, Volatile; D010936:Plant Extracts; D005054:Eugenol