title: The changing hydrodynamic performance of the decellularized intact porcine aortic root: considerations on in-vitro testing.
abstract: OBJECTIVE
  The most effective method for decellularization of the intact porcine aortic root remains controversial. Additionally, the hydrodynamic effect that such treatment may have on aortic roots has never been previously investigated. The study aim was to compare the in-vitro hydrodynamic performances of intact porcine aortic roots, both before and after decellularization treatment.
  
  
  METHODS
  Fifteen fresh porcine aortic roots were tested in the aortic chamber of the Sheffield pulse duplicator (SPD). For study purposes, the roots were first sutured to a silicone aortic root and then hydrodynamically tested. After in-vitro testing, the fresh porcine aortic roots, while still fixed within the silicone root, were decellularized according to various protocols (TRI-COL, TRI-DOC, sodium dodecyl sulfate (SDS) 0.03%, and SDS 0.1%). After decellularization, the valve roots were re-tested, adopting identical testing conditions. Forward flow pressure drop, closing leakage volumes, effective orifice area (EOA), and stroke work loss were each monitored. Three roots, used as a control group, were tested in identical fashion before and after storage (without decellularization) for comparative purposes.
  
  
  RESULTS
  The TRI-COL- and TRI-DOC-treated porcine aortic roots showed significantly lower transvalvular gradients, lower stroke work loss, lower valve resistance, and higher EOA than fresh intact porcine roots. In contrast, SDS 0.1%-treated porcine aortic roots showed opposing results, with the transvalvular gradients, stroke work loss and valve resistance each higher, and the EOA lower, than pre-treatment values. SDS 0.03% treatment had no significant effect on the hydrodynamic performance. After decellularization in all treatment groups, the diastolic parameters, total regurgitant volume and valve closing volume were each non-significantly increased. The aortic roots used as a control group showed similar results before and after storage.
  
  
  CONCLUSIONS
  Based on these results using the SPD, all treatments except for SDS 0.03% modified the systolic and diastolic functions of intact porcine aortic roots.
authors.affiliation: Cardiovascular Institute, University of Padova, Italy. tbottio@gmail.com
authors.forename: Tomaso
authors.initials: T
authors.lastname: Bottio
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Tarzia
authors.forename: Carlo
authors.initials: C
authors.lastname: Dal Lin
authors.forename: Edward
authors.initials: E
authors.lastname: Buratto
authors.forename: Giulio
authors.initials: G
authors.lastname: Rizzoli
authors.forename: Michele
authors.initials: M
authors.lastname: Spina
authors.forename: Alessandro
authors.initials: A
authors.lastname: Gandaglia
authors.forename: Filippo
authors.initials: F
authors.lastname: Naso
authors.forename: Gino
authors.initials: G
authors.lastname: Gerosa
mesh_terms: D000818:Animals; D001021:Aortic Valve / Q000166:cytology / Q000187:drug effects*; D001705:Bioprosthesis; D003840:Deoxycholic Acid / Q000494:pharmacology; D003902:Detergents / Q000494:pharmacology*; D006350:Heart Valve Prosthesis; D006439:Hemodynamics / Q000187:drug effects; D008422:Materials Testing; D017830:Octoxynol / Q000494:pharmacology; D011474:Prosthesis Design; D011475:Prosthesis Failure; D020358:Sodium Cholate / Q000494:pharmacology; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D013552:Swine; D054457:Tissue Scaffolds
chemical_list: D003902:Detergents; D003840:Deoxycholic Acid; D012967:Sodium Dodecyl Sulfate; D017830:Octoxynol; D020358:Sodium Cholate