Stable and efficient immobilization technique of aldolase under consecutive microwave irradiation at low temperature.
To establish a stable and efficient immobilization technique under microwave irradiation, a focused microwave reaction system was used, where the temperature was set appropriately in the microwave system and cooling module to produce consecutive microwave irradiation. 2-Deoxy-D-ribose-5-phosphate aldolase (DERA) was rapidly and efficiently immobilized in mesocellular siliceous foams (MCFs) under microwave irradiation. When the output power in the microwave system was set to 30 W, after 3 min, 88.4% of the enzyme protein was coupled to the wall of the support pores and the specific activity of the immobilized enzyme was 2.24 U mg(-1), 149.2% higher than that of the free enzyme and 157.0% higher than that of the non-microwave-assisted immobilized enzyme. In catalysis, microwave-assisted immobilized DERA tolerated a wider range of both pH and temperature than other DERA preparations. The thermal and storage stabilities were also significantly improved. This focused; microwave-assisted immobilization technique has proven to be simple, stable and highly efficient. This technique could also be applied to other enzyme immobilizations.
Wang
Anming
A
Research Center for Biomedicine and Health, Hangzhou Normal University, No. 222, Wenyi Road, Hangzhou 310012, PR China. anmwang@hotmail.com
Wang
Meizhang
M
Wang
Qiuyan
Q
Chen
Feifei
F
Zhang
Fangkai
F
Li
Haifeng
H
Zeng
Zhaowu
Z
Xie
Tian
T
D016227:Benzoquinones / Q000494:pharmacology; D001709:Biotechnology / Q000379:methods*; D003080:Cold Temperature; D003432:Cross-Linking Reagents / Q000494:pharmacology; D004795:Enzyme Stability / Q000187:drug effects; D004800:Enzymes, Immobilized / Q000378:metabolism*; D005634:Fructose-Bisphosphate Aldolase / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D007700:Kinetics; D008872:Microwaves; D016062:Porosity / Q000187:drug effects; D012822:Silicon Dioxide / Q000737:chemistry; D013997:Time Factors
D016227:Benzoquinones; D003432:Cross-Linking Reagents; D004800:Enzymes, Immobilized; D012822:Silicon Dioxide; D005634:Fructose-Bisphosphate Aldolase