Detecting protein-small molecule interactions using fluorous small-molecule microarrays.
General binding assays involving microarrays of small molecules can be used to identify small molecule ligands for nearly any protein, even in the absence of knowledge about protein structure or function. Several suitable methods for manufacturing small molecule microarrays (SMMs) exist and different immobilization methods may be more or less preferable for any given application. Here, we describe a protocol for noncovalent and homogenous capture of small molecules using fluorous interactions between small molecules containing fluorocarbon tags and fluorocarbon-coated glass surfaces. These arrays are especially useful for applications that require display of compounds in a specific orientation such as screening biased libraries.
Vegas
Arturo J
AJ
Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.
Koehler
Angela N
AN
D055162:Biocatalysis; D004353:Drug Evaluation, Preclinical; D057809:HEK293 Cells; D056572:Histone Deacetylase Inhibitors / Q000378:metabolism / Q000494:pharmacology; D006655:Histone Deacetylases / Q000302:isolation & purification / Q000378:metabolism; D006657:Histones / Q000378:metabolism; D006801:Humans; D006868:Hydrolysis; D046228:Microarray Analysis / Q000379:methods*; D056985:Nuclear Receptor Co-Repressor 2 / Q000378:metabolism; D011327:Printing; D011485:Protein Binding; D011506:Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D054852:Small Molecule Libraries / Q000378:metabolism* / Q000494:pharmacology
D056572:Histone Deacetylase Inhibitors; D006657:Histones; C539130:NCOR2 protein, human; D056985:Nuclear Receptor Co-Repressor 2; D011506:Proteins; D054852:Small Molecule Libraries; D006655:Histone Deacetylases