title: The relationship between meiotic spindle imaging and outcome of intracytoplasmic sperm injection: a retrospective study.
abstract: Meiotic spindle analysis with a non-invasive technique, the PolScope, is used to protect the meiotic spindle from damage during microinjection. To evaluate the predictive feature of PolScope, we have designed a retrospective study to analyse the correlation between the meiotic spindle visualisation with regard to spindle location and outcomes of assisted reproductive technologies (ART), including patient age, previous cycles, the number of the collected oocytes, fertilisation rates (FR), pronuclear scoring (PNS) and embryo scoring of the days from two to five. All of the data belonging to 1496 oocytes from 190 patients were statistically analysed. We found that the oocytes having PolScope visualised spindle have higher FR, and also observed that when the spindle located at 0°-30° according to the first polar body, gave the highest FR. PNS gave higher scores in the spindle visualised group, but spindle angle did not affect PNS outcomes. Although a correlation was found between spindle visualisation and developed embryo qualities, particularly at day 2 and 3, spindle angles did not affect embryo quality. We conclude that PolScope microscopy has an efficiency to estimate FR, and cleavage stage embryo development.
authors.affiliation: Gulhane Military Medical Faculty, IVF Centre, Etlik, 06010, Ankara, Turkey. cem_korkmaz_tr@yahoo.com
authors.forename: Cem
authors.initials: C
authors.lastname: Korkmaz
authors.forename: Ozgur
authors.initials: O
authors.lastname: Cinar
authors.forename: Mesut
authors.initials: M
authors.lastname: Akyol
mesh_terms: D000328:Adult; D000367:Age Factors; D001755:Blastocyst / Q000502:physiology / Q000648:ultrastructure; D002467:Cell Nucleus / Q000648:ultrastructure; D004477:Ectogenesis; D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D007247:Infertility, Female / Q000473:pathology* / Q000503:physiopathology* / Q000628:therapy; D008845:Microinjections / Q000009:adverse effects; D009865:Oocytes / Q000502:physiology / Q000648:ultrastructure; D010062:Ovulation Induction; D059705:Polar Bodies / Q000648:ultrastructure; D012189:Retrospective Studies; D020554:Sperm Injections, Intracytoplasmic; D008941:Spindle Apparatus / Q000648:ultrastructure*; D055815:Young Adult; D015053:Zygote / Q000502:physiology* / Q000648:ultrastructure*