title: Fibroblast growth factor-2 stimulates directed migration of periodontal ligament cells via PI3K/AKT signaling and CD44/hyaluronan interaction.
abstract: Fibroblast growth factor-2 (FGF-2) regulates a variety of functions of the periodontal ligament (PDL) cell, which is a key player during tissue regeneration following periodontal tissue breakdown by periodontal disease. In this study, we investigated the effects of FGF-2 on the cell migration and related signaling pathways of MPDL22, a mouse PDL cell clone. FGF-2 activated the migration of MPDL22 cells and phosphorylation of phosphatidylinositol 3-kinase (PI3K) and akt. The P13K inhibitors, Wortmannin and LY294002, suppressed both cell migration and akt activation in MPDL22, suggesting that the PI3K/akt pathway is involved in FGF-2-stimulated migration of MPDL22 cells. Moreover, in response to FGF-2, MPDL22 showed increased CD44 expression, avidity to hyaluronan (HA) partly via CD44, HA production and mRNA expression of HA synthase (Has)-1, 2, and 3. However, the distribution of HA molecular mass produced by MPDL22 was not altered by FGF-2 stimulation. Treatment of transwell membrane with HA facilitated the migration of MPDL22 cells and an anti-CD44 neutralizing antibody inhibited it. Interestingly, the expression of CD44 was colocalized with HA on the migrating cells when stimulated with FGF-2. Furthermore, an anti-CD44 antibody and small interfering RNA for CD44 significantly decreased the FGF-2-induced migration of MPDL22 cells. Taken together, PI3K/akt and CD44/HA signaling pathways are responsible for FGF-2-mediated cell motility of PDL cells, suggesting that FGF-2 accelerates periodontal regeneration by regulating the cellular functions including migration, proliferation and modulation of extracellular matrix production.
authors.affiliation: Division of Oral Biology and Disease Control, Department of Periodontology, Osaka University Graduate School of Dentistry, Osaka, Japan.
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Shimabukuro
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Terashima
authors.forename: Masahide
authors.initials: M
authors.lastname: Takedachi
authors.forename: Kenichiro
authors.initials: K
authors.lastname: Maeda
authors.forename: Tomomi
authors.initials: T
authors.lastname: Nakamura
authors.forename: Keigo
authors.initials: K
authors.lastname: Sawada
authors.forename: Mariko
authors.initials: M
authors.lastname: Kobashi
authors.forename: Toshihito
authors.initials: T
authors.lastname: Awata
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Oohara
authors.forename: Takanobu
authors.initials: T
authors.lastname: Kawahara
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Iwayama
authors.forename: Tomoko
authors.initials: T
authors.lastname: Hashikawa
authors.forename: Manabu
authors.initials: M
authors.lastname: Yanagita
authors.forename: Satoru
authors.initials: S
authors.lastname: Yamada
authors.forename: Shinya
authors.initials: S
authors.lastname: Murakami
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000187:drug effects*; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D020868:Gene Silencing / Q000187:drug effects; D006801:Humans; D018960:Hyaluronan Receptors / Q000378:metabolism*; D006820:Hyaluronic Acid / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D010513:Periodontal Ligament / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology; D058539:Phosphatidylinositol 3-Kinase / Q000378:metabolism*; D010766:Phosphorylation / Q000187:drug effects; D011485:Protein Binding / Q000187:drug effects; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D011554:Pseudopodia / Q000187:drug effects / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D018960:Hyaluronan Receptors; D034741:RNA, Small Interfering; D016222:Fibroblast Growth Factor 2; D006820:Hyaluronic Acid; D058539:Phosphatidylinositol 3-Kinase; D051057:Proto-Oncogene Proteins c-akt