[Expression of microRNA-146a in lipopolysaccharide challenged alveolar macrophages in vitro].
OBJECTIVE
To investigate the expression of microRNA-146a (miRNA-146a) in NR8383 alveolar macrophages treated with lipopolysaccharides (LPS).


METHODS
NR8383 alveolar macrophages were divided into two groups: LPS treated group and phosphate buffer saline (PBS) control group, and they cultured for 6 hours. The production of tumor necrosis factor-α (TNF-α) in the supernatant of cells was determined with enzyme-linked immunosorbent assay (ELISA), and the expression of miRNA-146a of cells was detected by real-time polymerase chain reaction (PCR).


RESULTS
Compared with PBS control group, the TNF-α content (ng/L) in LPS treated group was significantly increased (650.26±40.53 vs. 6.23±1.76, P<0.01), and miRNA-146a in LPS treated group increased by about (5.33±0.81) folds (P<0.01).


CONCLUSIONS
The expression of miRNA-146a was increased in LPS treated NR8383 cells, and miRNA-146a may be involved in the modulation inflammatory response of the NR8383 alveolar macrophage.
Liu
Fen
F
Intensive Care Unit, the First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi, China.
Zeng
Zhen-Guo
ZG
Ding
Cheng-Zhi
CZ
Shao
Qiang
Q
Li
Yong
Y
Qian
Ke-Jian
KJ
D000818:Animals; D002460:Cell Line; D007249:Inflammation; D008070:Lipopolysaccharides / Q000009:adverse effects; D016676:Macrophages, Alveolar / Q000187:drug effects / Q000378:metabolism*; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D008070:Lipopolysaccharides; C554180:MIRN146 microRNA, rat; D035683:MicroRNAs; D014409:Tumor Necrosis Factor-alpha