Evanescent wave fluorescence biosensor combined with DNA bio-barcode assay for platelet genotyping.
An evanescent wave fluorescence biosensor was combined with a DNA bio-barcode assay to resolve problems met in detection of poor biologic samples. Human platelet antigen (HPA) genotyping was used as a demonstrator. Our bio-barcode assay was based on magnetic carboxylatex particles and non-magnetic carboxylatex particles, both functionalized with oligonucleotides. It was assessed for detecting 84mer synthetic oligonucleotides as targets. The assay allows to specifically detect single nucleotide polymorphism with a detection limit of 2 pM of target nucleic acids. The fluorescence detection is achieved in 150 s.
Trévisan
Marie
M
Université de Lyon, Institut des Nanotechnologies de Lyon, UMR 5270, site Ecole Centrale de Lyon, 36 avenue Guy de Collongue, 69134 Ecully Cedex, France.
Schawaller
Manfred
M
Quapil
Gerald
G
Souteyrand
Eliane
E
Mérieux
Yves
Y
Cloarec
Jean-Pierre
JP
D016824:Antigens, Human Platelet / Q000235:genetics*; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D001792:Blood Platelets / Q000276:immunology*; D058893:DNA Barcoding, Taxonomic / Q000295:instrumentation / Q000379:methods*; D005453:Fluorescence; D005838:Genotype; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation / Q000379:methods; D015345:Oligonucleotide Probes; D020641:Polymorphism, Single Nucleotide
D016824:Antigens, Human Platelet; D015345:Oligonucleotide Probes