title: Molecular cloning of cyanobacterial pteridine glycosyltransferases that catalyze the transfer of either glucose or xylose to tetrahydrobiopterin.
abstract: Here, we report cloning of cyanobacterial genes encoding pteridine glycosyltransferases that catalyze glucosyl or xylosyl transfer from UDP-sugars to tetrahydrobiopterin. The genes were cloned by PCR amplification from genomic DNA which was isolated from culture and environmental samples and overexpressed in Escherichia coli for an in vitro activity assay.
authors.affiliation: School of Biological Sciences, Inje University, Kimhae 621-749, South Korea.
authors.forename: Yeol Gyun
authors.initials: YG
authors.lastname: Lee
authors.forename: Ae Hyun
authors.initials: AH
authors.lastname: Kim
authors.forename: Mi Bi
authors.initials: MB
authors.lastname: Park
authors.forename: Hye-Lim
authors.initials: HL
authors.lastname: Kim
authors.forename: Kon Ho
authors.initials: KH
authors.lastname: Lee
authors.forename: Young Shik
authors.initials: YS
authors.lastname: Park
mesh_terms: D000595:Amino Acid Sequence; D001708:Biopterins / Q000031:analogs & derivatives* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D003001:Cloning, Molecular; D016000:Cluster Analysis; D000458:Cyanobacteria / Q000201:enzymology* / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D004783:Environmental Microbiology; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D005947:Glucose / Q000378:metabolism*; D016695:Glycosyltransferases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014994:Xylose / Q000378:metabolism*
chemical_list: D004269:DNA, Bacterial; D001708:Biopterins; D014994:Xylose; D016695:Glycosyltransferases; C003402:sapropterin; D005947:Glucose