title: Casein kinase 2 and microtubules control axon initial segment formation.
abstract: The axon initial segment (AIS) is a unique axonal subdomain responsible for the generation of the neuronal action potential and the maintenance of the axon-dendritic functional polarity. Despite its importance, the mechanisms controlling AIS development and maintenance remain largely unknown. Here we show that the AIS microtubule cytoskeleton is composed of a pool of more stable, detergent resistant, microtubules. This AIS specific characteristic is conferred by the presence of CK2, an important regulator of microtubule stability, in the AIS during its development and maturation. We show that CK2α and CK2α' subunits concentrate at the AIS from its initial development, at the same time as pIκBα and ankyrinG. CK2 pharmacological inhibition or suppression of CK2α expression with nucleofected interference RNAs modifies microtubule characteristics throughout the neuron, changes KIF5C distribution, and impairs its own concentration at the AIS, as well as that of ankyrinG, ankyrinG-GFP, pIκBα and voltage gated sodium channels. Moreover, CK2α concentration at the AIS depends on IκBα phosphorylation by IKK and ankyrinG. In conclusion, our results demonstrate a mutual dependence of CK2, ankyrinG and pIκBα for their concentration at the axon initial segment, which is related to the specific characteristics of microtubules at the AIS.
authors.affiliation: Department of Cellular, Molecular and Developmental Neurobiology, Instituto Cajal, CSIC, 28002 Madrid, Spain.
authors.forename: Diana
authors.initials: D
authors.lastname: Sanchez-Ponce
authors.forename: Alberto
authors.initials: A
authors.lastname: Muñoz
authors.forename: Juan José
authors.initials: JJ
authors.lastname: Garrido
mesh_terms: D000200:Action Potentials / Q000502:physiology; D000818:Animals; D017487:Ankyrins / Q000235:genetics / Q000378:metabolism; D001369:Axons / Q000502:physiology* / Q000648:ultrastructure*; D047390:Casein Kinase II / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D006624:Hippocampus / Q000166:cytology; D020672:I-kappa B Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008870:Microtubules / Q000378:metabolism*; D000072000:NF-KappaB Inhibitor alpha; D021122:Protein Subunits / Q000378:metabolism; D034622:RNA Interference; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: C094681:Ank3 protein, mouse; D017487:Ankyrins; D020672:I-kappa B Proteins; C000606205:Nfkbia protein, mouse; D021122:Protein Subunits; D011993:Recombinant Fusion Proteins; D000072000:NF-KappaB Inhibitor alpha; D047390:Casein Kinase II