Photodynamic activity of aloe-emodin induces resensitization of lung cancer cells to anoikis.
Aloe-emodin was found to be a photosensitizer and possess anti-tumor activity. However, the detailed mechanism underlying the biological effects of aloe-emodin remains unknown. In this study, we explored the mechanisms of photocytotoxicity induced by aloe-emodin in lung cancer H460 cells. According to the results of the photoactivated aloe-emodin-induced disruption of cytoskeleton, we verify that aloe-emodin with irradiation induces anoikis of H460 cells. Photosensitized aloe-emodin-induced anoikis is associated with the protein expression of α-actinin and mitogen-activated protein (MAP) kinase members. In this study, a rapid opening of the mitochondrial permeability transition pore and the change in apoptosis-related protein expression were involved in photoactivated aloe-emodin-induced cell death. We also demonstrated that anoikis induced by aloe-emodin with irradiation is mediated through the intrinsic and extrinsic death pathways in a caspase-dependent manner in H460 cells.
Lee
Hong-Zin
HZ
School of Pharmacy, China Medical University, Taichung, Taiwan. hong@mail.cmu.edu.tw
Yang
Wen-Hui
WH
Hour
Mann-Jen
MJ
Wu
Chi-Yu
CY
Peng
Wen-Huang
WH
Bao
Bo-Ying
BY
Han
Ping-Hsiu
PH
Bau
Da-Tian
DT
D008841:Actin Cytoskeleton / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D000185:Actinin / Q000378:metabolism; D023102:Anoikis / Q000187:drug effects* / Q000528:radiation effects; D000880:Anthraquinones / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D020169:Caspases / Q000378:metabolism; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects / Q000528:radiation effects; D055551:HSP27 Heat-Shock Proteins / Q000378:metabolism; D006801:Humans; D008027:Light; D008175:Lung Neoplasms / Q000473:pathology*; D008870:Microtubules / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000494:pharmacology*; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
D000880:Anthraquinones; D000970:Antineoplastic Agents; D055551:HSP27 Heat-Shock Proteins; D017319:Photosensitizing Agents; D000185:Actinin; C518327:aloe emodin; D048051:p38 Mitogen-Activated Protein Kinases; D020169:Caspases