title: Enzymatic degradation of adhesive-dentin interfaces produced by mild self-etch adhesives.
abstract: Endogenous matrix metalloproteinases (MMPs) released by adhesive procedures may degrade collagen in the hybrid layer and so compromise the bonding effectiveness of etch-and-rinse adhesives. In this study, endogenous enzymatic degradation was evaluated for several simplified self-etch adhesives. In addition, primers were modified by adding two MMP inhibitors: chlorhexidine, a commonly used disinfectant, but also a non-specific MMP inhibitor; and SB-3CT, a specific inhibitor of MMP-2 and MMP-9. Gelatin zymography of fresh human dentin powder was used to identify the enzymes released by the adhesives. Micro-tensile bond strength (μTBS) testing was used to assess the mechanical properties of resin-dentin interfaces over time. In none of the experimental groups treated with the mild self-etch adhesives was MMP-2 and/or MMP-9 identified. Also, no difference in the μTBS was measured for the inhibitor-modified and the control inhibitor-free adhesives after 6 months of water storage. It is concluded that in contrast to etch-and-rinse adhesives, the involvement of endogenous MMP-2 and MMP-9 in the bond-degradation process is minimal for mild self-etch adhesives.
authors.affiliation: Department of Conservative Dentistry, Leuven BIOMAT Research Cluster, School of Dentistry, Oral Pathology and Maxillo-facial Surgery, Catholic University of Leuven, Leuven, Belgium. jan.demunck@med.kuleuven.be
authors.forename: Jan
authors.initials: J
authors.lastname: De Munck
authors.forename: Atsushi
authors.initials: A
authors.lastname: Mine
authors.forename: Philippe E
authors.initials: PE
authors.lastname: Van den Steen
authors.forename: Kirsten L
authors.initials: KL
authors.lastname: Van Landuyt
authors.forename: André
authors.initials: A
authors.lastname: Poitevin
authors.forename: Ghislain
authors.initials: G
authors.lastname: Opdenakker
authors.forename: Bart
authors.initials: B
authors.lastname: Van Meerbeek
mesh_terms: D000704:Analysis of Variance; D002710:Chlorhexidine / Q000494:pharmacology; D001840:Dental Bonding; D045662:Dental Etching / Q000379:methods*; D003799:Dental Stress Analysis; D003804:Dentin / Q000378:metabolism*; D017220:Dentin-Bonding Agents; D006573:Heterocyclic Compounds, 1-Ring / Q000494:pharmacology; D006801:Humans; D006868:Hydrolysis; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D061965:Matrix Metalloproteinase Inhibitors; D011480:Protease Inhibitors / Q000494:pharmacology; D019279:Resin Cements; D018709:Statistics, Nonparametric; D013450:Sulfones / Q000494:pharmacology; D013499:Surface Properties; D013718:Tensile Strength; D055815:Young Adult
chemical_list: D017220:Dentin-Bonding Agents; D006573:Heterocyclic Compounds, 1-Ring; D061965:Matrix Metalloproteinase Inhibitors; D011480:Protease Inhibitors; D019279:Resin Cements; C429533:SB 3CT compound; D013450:Sulfones; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; D002710:Chlorhexidine