title: Studies on cytotoxic constituents from the skin of the toad Bufo bufo gargarizans.
abstract: To study the chemical composition of the skin of Bufo bufo gargarizans, many kinds of chromatography methods were used in the isolation procedures, while the structures of isolated compounds were determined on the basis of their NMR and MS spectral analysis. As a result, two new compounds were isolated from its ethanolic extract and characterized as cinobufotalin 3-nonanedioylarginine ester (8) and bufotalin 3-pimeloylarginine ester (14). Furthermore, 13 known compounds were obtained. Isolated bufadienolides showed significant inhibition effect against SMMC-7721 cell lines in vitro.
authors.affiliation: Medical College, Xiamen University, Xiamen, China.
authors.forename: Hu-Yi
authors.initials: HY
authors.lastname: Zhao
authors.forename: Fu-Kai
authors.initials: FK
authors.lastname: Wu
authors.forename: Ying-Kun
authors.initials: YK
authors.lastname: Qiu
authors.forename: Zhen
authors.initials: Z
authors.lastname: Wu
authors.forename: Yong-Tao
authors.initials: YT
authors.lastname: Jiang
authors.forename: Ji-Yong
authors.initials: JY
authors.lastname: Chen
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D002018:Bufanolides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D002025:Bufonidae; D002681:China; D004354:Drug Screening Assays, Antitumor; D015394:Molecular Structure; D012867:Skin / Q000737:chemistry
chemical_list: D000970:Antineoplastic Agents; D002018:Bufanolides; C552791:bufotalin 3-pimeloylarginine ester; C552790:cinobufotalin 3-nonanedioylarginine ester; C004600:bufotalin