title: Radiotelemetric and symptomatic evaluation of pain in the rat after laparotomy: long-term benefits of perioperative ropivacaine care.
abstract: Effective relief of acute and long-term postoperative pain is of utmost importance to patients undergoing surgery. Here, we worked on a controlled procedure of abdominal surgery in the rat inducing persistent postoperative pain symptoms for up to 10 days and tested the efficacy of perioperative care with the local anesthetic ropivacaine. Laparotomy was likewise used to implant radiotelemetric probes by which electrocardiogram, body temperature, and locomotor activity were recorded in freely moving animals. We showed that postoperative pain symptoms (mechanical allodynia) measured in periphery of the scar were associated over time with persistent tachycardia, elevated heart rate variability, and loss of mobility. Furthermore, a single subcutaneous infiltration of the local anesthetic ropivacaine in the periphery of the abdominal incision was sufficient to prevent the appearance of allodynia and the associated cardiac and motor signs of pain, monitored by radiotelemetry. These beneficial effects were observed when the infiltration was performed in the perioperative period, but not later. This study on freely moving animals exhibiting long-lasting postoperative pain symptoms and altered autonomic/motor function illustrates well the importance of the timing of preemptive analgesia care with long-acting local anesthetics. Moreover, it emphasizes the utility of monitoring heart rate variability to quantify spontaneous expression of long-lasting postoperative pain.
  
  
  CONCLUSIONS
  Speeding the recovery time after surgery using perioperative ropivacaine care is of significant clinical relevance because it might limit the risk of chronic pain and postoperative complications. In humans, chronobiological analysis of heart rate variability could also help quantify spontaneous pain expression with minimal emotional bias.
authors.affiliation: Centre National de la Recherche Scientifique, Institut des Neurosciences Cellulaires et Intégratives, Unité Propre de Recherche 3212, Nociception and Pain Department, Strasbourg, France.
authors.forename: Alexandre
authors.initials: A
authors.lastname: Charlet
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Rodeau
authors.forename: Pierrick
authors.initials: P
authors.lastname: Poisbeau
mesh_terms: D000577:Amides / Q000494:pharmacology* / Q000627:therapeutic use; D000779:Anesthetics, Local / Q000494:pharmacology / Q000627:therapeutic use; D000818:Animals; D004195:Disease Models, Animal; D007813:Laparotomy / Q000009:adverse effects*; D008297:Male; D010147:Pain Measurement / Q000379:methods*; D010149:Postoperative Pain / Q000175:diagnosis* / Q000188:drug therapy*; D011182:Postoperative Care / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D000077212:Ropivacaine; D013686:Telemetry / Q000295:instrumentation / Q000379:methods*; D013995:Time
chemical_list: D000577:Amides; D000779:Anesthetics, Local; D000077212:Ropivacaine