Genetic aberrations of the K-ras proto-oncogene in bladder cancer in Kashmiri population.
OBJECTIVE
To assess the frequency of specific point mutations in the K-ras gene in a group of Kashmiri patients with bladder cancer.


METHODS
We analyzed the incidence of K-ras exon 1 gene mutations in tumors and surgical margins in 60 patients with transitional cell carcinoma of varied clinical stages and histological grades using the polymerase chain reaction-single strand conformation polymorphism and DNA sequencing.


RESULTS
A significant correlation was found between the K-ras, the lymph node status, and tumor recurrence (P < 0.05). Also, smokers and patients with higher tumor grade showed a significantly higher relative risk of developing K-ras mutations than the normal ones.


CONCLUSIONS
K-ras exon 1 gene mutations were found with low frequency in the bladder cancer tumors from Kashmir valley, which suggests that K-ras gene might be involved in a sub-set of bladder tumors, but it needs further investigation on a larger cohort sample to authenticate the current findings.
Nanda
Mahoor S
MS
Department of Biosciences, Jamia Millia Islamia, Jamia Nagar, New Delhi, India.
Sameer
A Syed
AS
Syeed
Nidda
N
Shah
Zaffar A
ZA
Murtaza
Imtiyaz
I
Siddiqi
Mushtaq A
MA
Ali
Arif
A
D002295:Carcinoma, Transitional Cell / Q000453:epidemiology / Q000235:genetics* / Q000378:metabolism; D002869:Chromosome Aberrations; D004273:DNA, Neoplasm / Q000235:genetics*; D005260:Female; D011905:Genes, ras / Q000235:genetics*; D006801:Humans; D007194:India / Q000453:epidemiology; D008297:Male; D008875:Middle Aged; D009154:Mutation; D016133:Polymerase Chain Reaction; D015995:Prevalence; D000090063:Proto-Oncogene Mas; D001749:Urinary Bladder Neoplasms / Q000453:epidemiology / Q000235:genetics* / Q000378:metabolism
D004273:DNA, Neoplasm; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas