Comparative field analyses of rapid analyte measurement platform and reverse transcriptase polymerase chain reaction assays for West Nile virus surveillance.
Rapid detection of West Nile virus (WNV) in mosquito pools is essential for predicting epizootics and epidemics. We compare the efficiency and sensitivity of the Rapid Analyte Measurement Platform (RAMP) to reverse transcriptase polymerase chain reaction (RT-PCR) from 2005 to 2008 from field mosquito populations in Mercer County, NJ. Overall, 316 pools tested negative and 115 pools tested positive for WNV. Eighty-nine pools tested positive using RAMP and all were confirmed by RT-PCR; 26 pools were WNV-negative using RAMP but positive using RT-PCR. False-positives from RAMP were not detected in our four-year study, indicating that RAMP is a reliable tool when used to augment existing RT-PCR-based WNV surveillance programs. Local mosquito control programs using RAMP will benefit from its ease of use, quick results, and lack of false positives but should understand the sensitivity of this test when compared to RT-PCR. Used with standard methods, RAMP will enhance existing mosquito control and WNV surveillance by providing rapid results and improved mosquito management decisions.
Williges
Eric
E
Center for Vector Biology, Department of Entomology, Rutgers University, 180 Jones Avenue, New Brunswick, NJ 08901, USA.
Farajollahi
Ary
A
Nelder
Mark P
MP
Gaugler
Randy
R
D000818:Animals; D009033:Culicidae / Q000821:virology*; D005260:Female; D007124:Immunoenzyme Techniques; D007303:Insect Vectors / Q000821:virology*; D008137:Longitudinal Studies; D009032:Mosquito Control; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014901:West Nile Fever / Q000635:transmission; D014902:West Nile virus / Q000235:genetics / Q000302:isolation & purification*