title: Oct4 regulates the miR-302 cluster in P19 mouse embryonic carcinoma cells.
abstract: Oct4 is a transcription factor that is required for pluripotency during early embryogenesis and the maintenance of embryonic stem (ES) cell and pluripotent cell identity. miR-302, a cluster of eight microRNAs (miRNAs) that are expressed specifically in ES cells and pluripotent cells, is crucial for normal pluripotent cell self-renewal and pluripotency. But, the mechanism by which miR-302 participates in the core regulatory circuitry that controls self-renewal and pluripotency in P19 embryonic carcinoma cells has not been established. Here, we show that Oct4 is required for the expression and transcriptional activation of miR-302 and that Oct4 binds to the putative promoter of miR-302, suggesting that Oct4 activates the primary miR-302 transcript in P19 cells. This study proposes that the miR-302 cluster acts downstream of the Oct4 regulation network in P19 cells.
authors.affiliation: Department of Reproductive Medicine, First Affiliated Hospital of Harbin Medical University, Harbin, 150001 Heilongjiang, People's Republic of China.
authors.forename: Haiying
authors.initials: H
authors.lastname: Liu
authors.forename: Suo
authors.initials: S
authors.lastname: Deng
authors.forename: Zihan
authors.initials: Z
authors.lastname: Zhao
authors.forename: Huiya
authors.initials: H
authors.lastname: Zhang
authors.forename: Juxia
authors.initials: J
authors.lastname: Xiao
authors.forename: Wei
authors.initials: W
authors.lastname: Song
authors.forename: Fei
authors.initials: F
authors.lastname: Gao
authors.forename: Yongmei
authors.initials: Y
authors.lastname: Guan
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D047369:Chromatin Immunoprecipitation; D054278:Embryonal Carcinoma Stem Cells / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D051379:Mice; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D005810:Multigene Family; D050814:Octamer Transcription Factor-3 / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding; D014158:Transcription, Genetic
chemical_list: C558265:MIRN302 microRNA, mouse; D035683:MicroRNAs; D050814:Octamer Transcription Factor-3; C494174:Pou5f1 protein, mouse