The genome-wide expression profile of Curcuma longa-treated cisplatin-stimulated HEK293 cells.
OBJECTIVE
The rhizome of turmeric, Curcuma longa (CL), is a herbal medicine used in many traditional prescriptions. It has previously been shown that CL treatment showed greater than 47% recovery from cisplatin-induced cell damage in human kidney HEK 293 cells. This study was conducted to evaluate the recovery mechanisms of CL that occur during cisplatin induced nephrotoxicity by examining the genome wide mRNA expression profiles of HEK 293 -cells.


METHODS
Recovery mechanisms of CL that occur during cisplatin-induced nephrotoxicity were determined by microarray, real-time PCR, immunofluorescent confocal microscopy and Western blot analysis.


RESULTS
The results of microarray analysis and real-time PCR revealed that NFκB pathway-related genes and apoptosis-related genes were down-regulated in CL-treated HEK 293 cells. In addition, immunofluorescent confocal microscopy and Western blot analysis revealed that NFκB p65 nuclear translocation was inhibited in CL-treated HEK 293 cells. Therefore, the mechanism responsible for the effects of CL on HEK 293 cells is closely associated with regulation of the NFκB pathway.


CONCLUSIONS
CL possesses novel therapeutic agents that can be used for the prevention or treatment of cisplatin-induced renal disorders.
Sohn
Sung-Hwa
SH
Department of Physiology, College of Oriental Medicine Department of Oriental Pathology, College of Oriental Medicine, KyungHee University, Seoul 130-701, Republic of Korea.
Ko
Eunjung
E
Chung
Hwan-Suck
HS
Lee
Eun-Young
EY
Kim
Sung-Hoon
SH
Shin
Minkyu
M
Hong
Moochang
M
Bae
Hyunsu
H
D000970:Antineoplastic Agents / Q000633:toxicity*; D002460:Cell Line / Q000378:metabolism*; D002945:Cisplatin / Q000633:toxicity*; D030024:Curcuma / Q000737:chemistry*; D015536:Down-Regulation; D020869:Gene Expression Profiling; D016678:Genome / Q000187:drug effects; D006801:Humans; D007674:Kidney Diseases / Q000139:chemically induced* / Q000235:genetics; D046228:Microarray Analysis; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism*; D010936:Plant Extracts / Q000494:pharmacology*; D016133:Polymerase Chain Reaction / Q000379:methods
D000970:Antineoplastic Agents; D016328:NF-kappa B; D010936:Plant Extracts; D002945:Cisplatin