Protein kinase A in the pedunculopontine tegmental nucleus of rat contributes to regulation of rapid eye movement sleep.
Intracellular signaling mechanisms within the pedunculopontine tegmental (PPT) nucleus for the regulation of recovery rapid eye movement (REM) sleep following REM sleep deprivation remain unknown. This study was designed to determine the role of PPT intracellular cAMP-dependent protein kinase A (cAMP-PKA) in the regulation of recovery REM sleep in freely moving rats. The results show that a brief period (3 h) of selective REM sleep deprivation caused REM sleep rebound associated with increased PKA activity and expression of the PKA catalytic subunit protein (PKA-CU) in the PPT. Local application of a cAMP-PKA-activation-selective inhibitor, RpCAMPS (0.55, 1.1, and 2.2 nmol/100 nl; n = 8 rats/group), bilaterally into the PPT, reduced PKA activity and PKA-CU expression in the PPT, and suppressed the recovery REM sleep, in a dose-dependent manner. Regression analyses revealed significant positive relationships between: PPT levels of PKA activity and the total percentages of REM sleep recovery (Rsqr = 0.944; n = 40 rats); PPT levels of PKA-CU expression and the total percentages of REM sleep recovery (Rsqr = 0.937; n = 40 rats); PPT levels of PKA-CU expression and PKA activity (Rsqr = 0.945; n = 40 rats). Collectively, these results provide evidence that activation of intracellular PKA in the PPT contributes to REM sleep recovery following REM sleep deprivation.
Datta
Subimal
S
Laboratory of Sleep and Cognitive Neuroscience, Department of Psychiatry, Boston University School of Medicine, Boston, Massachusetts 02118, USA. Subimal@bu.edu
Desarnaud
Frank
F
D000818:Animals; D054751:Cyclic AMP-Dependent Protein Kinase Catalytic Subunits / Q000037:antagonists & inhibitors / Q000502:physiology*; D004789:Enzyme Activation / Q000502:physiology; D007424:Intracellular Fluid / Q000201:enzymology; D008297:Male; D009474:Neurons / Q000201:enzymology; D045042:Pedunculopontine Tegmental Nucleus / Q000166:cytology / Q000201:enzymology*; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D021122:Protein Subunits / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D020127:Recovery of Function / Q000502:physiology; D012892:Sleep Deprivation / Q000201:enzymology* / Q000503:physiopathology; D012895:Sleep, REM / Q000502:physiology*
D047428:Protein Kinase Inhibitors; D021122:Protein Subunits; D054751:Cyclic AMP-Dependent Protein Kinase Catalytic Subunits; C517759:Prkaca protein, rat