title: Acute graft-vs.-host disease correlates with the disparity for the PECAM-1 S536N polymorphism only in the HLA-B44-like positive Tunisian recipients of HSCs.
abstract: GVHD is the major cause of mortality after HLA-identical HSCT. Such complication has been widely linked to donor/recipient disparity for minor histocompatibility antigens (MiHAgs). PECAM-1 is one of potential human MiHAgs but its effect on the GVHD occurrence remains not clear. In order to examine such association in the Tunisian cohort of HSCs recipients, we performed a retrospective study on patients who undergone HLA-identical HSCT between 2000 and 2009. Genotyping of the three selected PECAM-1 polymorphisms (rs668, rs12953 and rs1131012) was performed with SSP-PCR method. Univariate analyses showed that grades II-IV acute GVHD were considerably linked to the non-identity for rs12953 only in HLA-B44-like positive patients (p=0.010, OR=10.000). Multivariate analysis for chronic GVHD showed that this outcome may be affected only by the adulthood and the conditioning regimen. Our findings support the previously reported data suggesting a significant association between the PECAM-1 disparity and the risk of acute GVHD.
authors.affiliation: The "Immunogenetic Applied to Cells Therapy" Research Unit, The Immuno-Haematology Department, National Blood Transfusion Centre of Tunis, Tunisia. sellamimh@laposte.net
authors.forename: Mohamed Hichem
authors.initials: MH
authors.lastname: Sellami
authors.forename: Saloua
authors.initials: S
authors.lastname: Ladeb
authors.forename: Houda
authors.initials: H
authors.lastname: Kaabi
authors.forename: Ghaya
authors.initials: G
authors.lastname: Cherif
authors.forename: Lamia
authors.initials: L
authors.lastname: Torjemane
authors.forename: Tarek Ben
authors.initials: TB
authors.lastname: Othman
authors.forename: Slama
authors.initials: S
authors.lastname: Hmida
mesh_terms: D000208:Acute Disease; D006086:Graft vs Host Disease / Q000235:genetics / Q000276:immunology; D015235:HLA-B Antigens / Q000235:genetics / Q000276:immunology; D059948:HLA-B44 Antigen; D018380:Hematopoietic Stem Cell Transplantation; D006648:Histocompatibility; D006801:Humans; D015778:Minor Histocompatibility Antigens; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000235:genetics* / Q000276:immunology; D011110:Polymorphism, Genetic; D012189:Retrospective Studies; D014416:Tunisia
chemical_list: D015235:HLA-B Antigens; D059948:HLA-B44 Antigen; D015778:Minor Histocompatibility Antigens; D019408:Platelet Endothelial Cell Adhesion Molecule-1