Highly sensitive identification of cancer cells by combining the new tetrathiafulvalene derivative with a β-cyclodextrin/multi-walled carbon nanotubes modified GCE.
In this contribution, we have prepared and explored a novel nano-interface based probe for the rapid identification and highly sensitive detection of cancer cells by means of an electrochemical study. The new probe tetrathiafulvalene (TTF) carboxylate salt (TTF-(COONBu(4))(2), ditetrabutylammonium salt for propylenedithio-4',5'-tetrathiafulvalene-4,5-dicarboxylate), which has specific spectral and electrochemical properties, has been synthesized and assembled with carbon nanotubes to form a new type of nanocomposite. A simple method of fabricating the β-CD/MWCNT modified electrodes based on functionalized multi-walled carbon nanotubes (MWCNTs) and β-cyclodextrin (β-CD) has been explored by using glassy carbon electrodes (GCEs), which could remarkably enhance the sensitivity of the biomolecular detection. Our results demonstrate that the combination of the new probe TTF-(COONBu(4))(2) with β-CD/MWCNT modified electrodes could be readily utilized to sensitively detect cancer cells such as liver cancer cells SMMC-7721 and HepG2, drug sensitive leukemia K562/B.W cells and drug resistant leukemia K562/ADM cells, with a detection limit of ~10(3) cells mL(-1). This may provide a novel strategy for the potential and promising application of the new TTF molecular probe in the development of multi-signal responsive biosensors for the early diagnosis of cancers.
Zhao
Juan
J
State Key Laboratory of Bioelectronics (Chien-Shiung Wu Laboratory), Southeast University, Nanjing 210096, People's Republic of China.
Jin
Jianshi
J
Wu
Chunhui
C
Jiang
Hui
H
Zhou
Yanyan
Y
Zuo
Jinglin
J
Wang
Xuemei
X
D002244:Carbon / Q000737:chemistry*; D002478:Cells, Cultured; D004563:Electrochemistry; D004566:Electrodes; D005898:Glass / Q000737:chemistry; D006573:Heterocyclic Compounds, 1-Ring / Q000138:chemical synthesis / Q000737:chemistry*; D006801:Humans; D015394:Molecular Structure; D037742:Nanotubes, Carbon / Q000737:chemistry*; D009369:Neoplasms / Q000175:diagnosis* / Q000473:pathology*; D010316:Particle Size; D012680:Sensitivity and Specificity; D013237:Stereoisomerism; D013499:Surface Properties; D047392:beta-Cyclodextrins / Q000737:chemistry*
D006573:Heterocyclic Compounds, 1-Ring; D037742:Nanotubes, Carbon; D047392:beta-Cyclodextrins; D002244:Carbon; C031215:betadex