title: Rhizobium herbae sp. nov. and Rhizobium giardinii-related bacteria, minor microsymbionts of various wild legumes in China.
abstract: Seven Rhizobium strains associated with various legume species grown in different geographical regions of China were defined into four genomic groups related to Rhizobium giardinii, based upon ribosomal intergenic spacer RFLP, phylogenies of 16S rRNA and housekeeping (atpD, recA and glnII) genes, and DNA relatedness. Three strains in group I were classified as R. giardinii, as they showed high gene sequence similarities (>97 %) and DNA relatedness (64.3-67.5 %) to R. giardinii H152(T). Groups II, III and IV differed from all defined Rhizobium species based upon the consensus of all analyses. As group II contained two strains that originated from two distinct populations, we propose this group as a novel species, Rhizobium herbae sp. nov., with strain CCBAU 83011(T) ( = LMG 25718(T) = HAMBI 3117(T)) as the type strain.
authors.affiliation: State Key Laboratories for Agrobiotechnology/College of Biological Sciences, China Agricultural University, Beijing 100193, PR China.
authors.forename: Da Wei
authors.initials: DW
authors.lastname: Ren
authors.affiliation: Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, 11340 México D. F., Mexico.|State Key Laboratories for Agrobiotechnology/College of Biological Sciences, China Agricultural University, Beijing 100193, PR China.
authors.forename: En Tao
authors.initials: ET
authors.lastname: Wang
authors.affiliation: State Key Laboratories for Agrobiotechnology/College of Biological Sciences, China Agricultural University, Beijing 100193, PR China.
authors.forename: Wen Feng
authors.initials: WF
authors.lastname: Chen
authors.affiliation: State Key Laboratories for Agrobiotechnology/College of Biological Sciences, China Agricultural University, Beijing 100193, PR China.
authors.forename: Xin Hua
authors.initials: XH
authors.lastname: Sui
authors.affiliation: Agricultural Culture Collection of China, Institute of Agricultural Resource and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 10081, PR China.
authors.forename: Xiao Xia
authors.initials: XX
authors.lastname: Zhang
authors.affiliation: Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Hong Can
authors.initials: HC
authors.lastname: Liu
authors.affiliation: State Key Laboratories for Agrobiotechnology/College of Biological Sciences, China Agricultural University, Beijing 100193, PR China.
authors.forename: Wen Xin
authors.initials: WX
authors.lastname: Chen
mesh_terms: D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D007887:Fabaceae / Q000382:microbiology* / Q000502:physiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012231:Rhizobium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D013559:Symbiosis
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S