title: Improved glucose electrochemical biosensor by appropriate immobilization of nano-ZnO.
abstract: We constructed the transferred ZnO biosensor and the grown ZnO biosensor by two different nano-ZnO immobilization approaches. And the influence of different assembly processes on the biosensor performance has been systematically investigated and compared. An enhanced sensitivity of the grown ZnO biosensor is found to be 52% higher than that of the transferred ZnO biosensor. Correspondingly, the other properties are also better in the grown ZnO biosensor, including the response time, the detection limit and the linear range. These results are well consistent with the fact that more glucose oxidase is immobilized on the well-aligned ZnO arrays, which have higher specific surface area and more direct electron communication path, in the grown sensor than the randomly distributed and stacked ZnO nanorods in the transferred sensor. The nano-ZnO grown directly has been demonstrated more desirable for enzymatic immobilization and signal transduction in the high performance biosensors.
authors.affiliation: State Key Laboratory for Advanced Metals and Materials, School of Materials Science & Engineering, University of Science & Technology Beijing 100083, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Lei
authors.forename: Xiaoqin
authors.initials: X
authors.lastname: Yan
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
authors.forename: Xi
authors.initials: X
authors.lastname: Liu
authors.forename: Yu
authors.initials: Y
authors.lastname: Song
authors.forename: Ning
authors.initials: N
authors.lastname: Luo
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D002138:Calibration; D004563:Electrochemistry / Q000295:instrumentation*; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000378:metabolism; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000378:metabolism; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D043942:Nanotubes / Q000737:chemistry; D012996:Solutions; D013499:Surface Properties; D015034:Zinc Oxide / Q000737:chemistry*
chemical_list: D004800:Enzymes, Immobilized; D012996:Solutions; D005949:Glucose Oxidase; D005947:Glucose; D015034:Zinc Oxide