title: Combined peptides of human enterovirus 71 protect against virus infection in mice.
abstract: Human enterovirus 71 (EV71) is a cause of hand, foot and mouth disease (HMFD) in children under 6 years old, and could cause serious neurological complications in some patients. Numerous large outbreaks of EV71 caused HMFD have occurred recently in Asia, especially in China. The cross-reactivity of EV71 with human brain tissue was observed and the cross-reactivity inducing regions were identified in previously study, which suggested that there were two regions in structural proteins of virus should be avoided in the vaccine. Six peptides without cross-reactivity were selected and combined into three vaccine candidates and applied in further evaluation in neonatal mice. The Vac6 comprising the peptides of P(70-159), P(140-249), P(324-443) and P(746-876) of the structural proteins could provide effective protection on pups against virus infection, as shown in viral copies detection and histopathology examination. Immunohistochemical staining results indicated that Vac6 had no cross-reactivity with human brain tissues. Our results suggested that Vac6 could have potential clinical value against EV71 epidemics caused mainly by C4 strains in the mainland of China.
authors.affiliation: Key Laboratory of Human Diseases Comparative Medicine, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences (CAMS) & Comparative Medicine Centre, Peking Union Medical Collage (PUMC), Beijing 100021, China. ljn zb03038@126.com
authors.forename: Jiang Ning
authors.initials: JN
authors.lastname: Liu
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Jian Ying
authors.initials: JY
authors.lastname: Duo
authors.forename: Yi
authors.initials: Y
authors.lastname: Hao
authors.forename: Chun Mei
authors.initials: CM
authors.lastname: Ma
authors.forename: Wan Bo
authors.initials: WB
authors.lastname: Li
authors.forename: Shu Zhu
authors.initials: SZ
authors.lastname: Lin
authors.forename: Xue Zhong
authors.initials: XZ
authors.lastname: Gao
authors.forename: Xiao Lin
authors.initials: XL
authors.lastname: Liu
authors.forename: Yan Feng
authors.initials: YF
authors.lastname: Xu
authors.forename: Wen-Bo
authors.initials: WB
authors.lastname: Xu
authors.forename: Chuan
authors.initials: C
authors.lastname: Qin
authors.forename: Lian Feng
authors.initials: LF
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D057134:Antibodies, Neutralizing / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood; D001921:Brain / Q000276:immunology / Q000821:virology; D003429:Cross Reactions; D004195:Disease Models, Animal; D029821:Enterovirus A, Human / Q000276:immunology*; D004769:Enterovirus Infections / Q000276:immunology / Q000517:prevention & control*; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009500:Neutralization Tests; D010455:Peptides / Q000276:immunology*; D015678:Viral Structural Proteins / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D010455:Peptides; D015678:Viral Structural Proteins; D014765:Viral Vaccines