Potential of the hepatic transcriptome expression profile of the striped seabream (Lithognathus mormyrus) as an environmental biomarker.
The potential of the hepatic transcriptome expression profile evaluated in a sentinel feral fish to serve as an environmental biomarker was examined. Expression profiles of Lithognathus mormyrus individuals were exhibited using cDNA microarray and were related to the set of exposure conditions at their sites and dates of collection. Expression profiles of individual fish were reasonably clustered according to the fish samples. In addition, several sample-specific gene clusters were determined, designated sample gene signatures. The selection procedure for future optimal reference RNA is discussed. The relationship between transcriptome expression and fish samples indicated a potential for using the former as an environmental biomarker.
Yudkovski
Yana
Y
Israel Oceanographic and Limnological Research, Haifa, Israel.
Ramšak
Andreja
A
Ausland
Meirav
M
Tom
Moshe
M
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D004784:Environmental Monitoring / Q000379:methods*; D020869:Gene Expression Profiling; D008099:Liver / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D021541:Sea Bream
D017931:DNA Primers