title: Hydrolysates of citrus plants stimulate melanogenesis protecting against UV-induced dermal damage.
abstract: The sun-tanning process occurs as a spontaneous response to ultraviolet (UV) irradiation. UV will induce tanning and DNA damage, processes that can lead to photoaging and skin disorders such as hyperpigmentation and cancer. The pigment melanin protects skin from UV damage; therefore, an efficient melanin-promoting suntan lotion could be highly beneficial. In this study, a process was developed to increase the content of naringenin in citrus extracts and to determine whether a higher naringenin content of citrus would induce melanogenesis. Melanin content and tyrosinase expression in mouse B16 melanoma cells were assayed after treatment with citrus plant extracts and their hydrolysates. The results indicate that hydrolysis increased the naringenin content in citrus extracts and that citrus preparations stimulated cellular melanogenesis and tyrosinase expression. It is suggested that this method is applicable to the industrial production of melanin-promoting suntan lotions with antiphotocarcinogenic properties derived from citrus rind and citrus products.
authors.affiliation: Department of Cosmeceutics, China Medical University, Taichung, Taiwan.
authors.forename: Hsiu-Mei
authors.initials: HM
authors.lastname: Chiang
authors.forename: Jen-Wen
authors.initials: JW
authors.lastname: Lin
authors.forename: Pei-Ling
authors.initials: PL
authors.lastname: Hsiao
authors.forename: Shang-Yuan
authors.initials: SY
authors.lastname: Tsai
authors.forename: Kuo-Ching
authors.initials: KC
authors.lastname: Wen
mesh_terms: D000818:Animals; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002851:Chromatography, High Pressure Liquid; D002957:Citrus / Q000378:metabolism*; D006868:Hydrolysis; D008543:Melanins / Q000096:biosynthesis* / Q000378:metabolism; D008546:Melanoma, Experimental / Q000201:enzymology / Q000378:metabolism / Q000473:pathology; D051379:Mice; D014442:Monophenol Monooxygenase / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D012867:Skin / Q000187:drug effects* / Q000528:radiation effects*; D014466:Ultraviolet Rays
chemical_list: D008543:Melanins; D010936:Plant Extracts; D014442:Monophenol Monooxygenase