title: In vitro research of the alteration of neurons in vagal core in medulla oblongata at asphyxic deaths.
abstract: The aim of this study was to research the morphological changes of neurons in the vagus nerve nuclei in medulla oblongata in asphyxia related death cases. Morphological changes that were investigated were mainly in the dorsal motor respiratory center (DMRC), nucleus tractus solitarius (nTS) and nucleus ambigus (nA) in the medulla oblongata. In our research, the autopsy material from asphyxia related death cases was used from various etiologies: monoxide carbon (CO), liquid drowning, strangulation, electricity, clinical-pathological death, firing weapon, explosive weapon, sharp and blunt objects and death cases due to accident. The material selected for research was taken from medulla oblongata and lungs from all lobes. The material from the medulla oblongata and lungs was fixed in a 10% solution of buffered formalin. Special histochemical methods for central nervous system (CNS) were employed like: Cresyl echt violet, toluidin blue, Sevier-Munger modification and Grimelius. For stereometrical analysis of the quantitative density of the neurons the universal testing system Weibel M42 was used. The acquired results show that in sudden asphyxia related death cases, there are alterations in the nuclei of vagal nerve in form of: central chromatolysis, axonal retraction, axonal fragmentation, intranuclear vacuolization, cytoplasmic vacuolization, edema, condensation and dispersion of substance of Nissl, proliferation of oligodendrocytes, astrocytes and microglia. The altered population of vagus nerve neurons does not show an important statistical significance compared to the overall quantity of the neurons in the nuclei of the vagus nerve (p<0.05).
authors.affiliation: Department of Forensic Medicine, Faculty of Medicine, University of Prishtina, Clinical Centre N.N. 10000, Prishtina, Kosovo.
authors.forename: Naim
authors.initials: N
authors.lastname: Haliti
authors.forename: Hilmi
authors.initials: H
authors.lastname: Islami
authors.forename: Nevzat
authors.initials: N
authors.lastname: Elezi
authors.forename: Ragip
authors.initials: R
authors.lastname: Shabani
authors.forename: Bedri
authors.initials: B
authors.lastname: Abdullahu
authors.forename: Gani
authors.initials: G
authors.lastname: Dragusha
mesh_terms: D000059:Accidents; D001237:Asphyxia / Q000473:pathology*; D002249:Carbon Monoxide Poisoning / Q000473:pathology; D002452:Cell Count; D004332:Drowning / Q000473:pathology; D006801:Humans; D008168:Lung / Q000473:pathology; D008526:Medulla Oblongata / Q000473:pathology*; D009474:Neurons / Q000473:pathology*; D013405:Suicide; D014630:Vagus Nerve / Q000473:pathology*; D014754:Violence; D054041:Weapons