Shewanella indica sp. nov., isolated from sediment of the Arabian Sea.
A Gram-negative, facultatively anaerobic, rod-shaped, catalase- and oxidase-positive bacterium, motile by means of a single polar flagellum and designated strain KJW27(T), was isolated from the marine sediment of Karwar jetty, west coast of India. The strain was β-haemolytic and grew with 0-10 % (w/v) NaCl, at 10-45 °C and at pH 6.5-10, with optimum growth with 2 % (w/v) NaCl, at 37 °C and at pH 7.5. The major fatty acids were iso-C₁₅:₀ (22.2 %), C₁₇:₁ω8c (21 %), summed feature 3 (comprising C₁₆:₁ω7c and/or C₁₆:₁ω6c; 10.2 %), C₁₆:₀ (7.1 %), iso-C₁₃:₀ (5.6 %) and C₁₇:₀ (4.4 %). The DNA G+C content was 51.2 mol%. Phylogenetic analysis based on 16S rRNA and gyrB gene sequences showed that strain KJW27(T) forms a lineage within the genus Shewanella and is closely related to Shewanella algae ATCC 51192(T) (98.8 %), Shewanella haliotis DW01(T) (98.8 %) and Shewanella chilikensis JC5(T) (98.2 %). Sequence identity with other members of this genus ranges from 92.2 to 96.4 %. The DNA-DNA relatedness of strain KJW27(T) with S. algae ATCC 51192(T), S. haliotis DW01(T) and S. chilikensis JC5(T) was 52, 44 and 33 %, respectively. The phenotypic, genotypic and DNA-DNA relatedness data indicate that strain KJW27(T) should be distinguished from S. algae ATCC 51192(T), S. haliotis DW01(T) and S. chilikensis JC5(T). On the basis of the data presented in this study, strain KJW27(T) represents a novel species, for which the name Shewanella indica sp. nov. is proposed. The type strain is KJW27(T) ( = KCTC 23171(T)  = BCC 41031(T)  = NCIM 5388(T)).
Verma
Pankaj
P
Molecular Biology Unit, National Centre for Cell Science, University of Pune, Pune 411 007, Maharashtra, India.
Pandey
Prashant Kumar
PK
Molecular Biology Unit, National Centre for Cell Science, University of Pune, Pune 411 007, Maharashtra, India.
Gupta
Arvind Kumar
AK
Molecular Biology Unit, National Centre for Cell Science, University of Pune, Pune 411 007, Maharashtra, India.
Kim
Ho Jun
HJ
Department of Biology, College of Life Science and Natural Resources, Sunchon National University, Suncheon 540-742, Republic of Korea.
Baik
Keun Sik
KS
Department of Biology, College of Life Science and Natural Resources, Sunchon National University, Suncheon 540-742, Republic of Korea.
Seong
Chi Nam
CN
Department of Biology, College of Life Science and Natural Resources, Sunchon National University, Suncheon 540-742, Republic of Korea.
Patole
Milind Shivaji
MS
Molecular Biology Unit, National Centre for Cell Science, University of Pune, Pune 411 007, Maharashtra, India.
Shouche
Yogesh Shreepad
YS
Microbial Culture Collection, Hindustan Antibiotics Ltd. Complex, Pimpri, Pune 411 018, Maharashtra, India.|Molecular Biology Unit, National Centre for Cell Science, University of Pune, Pune 411 007, Maharashtra, India.
D000332:Aerobiosis; D000693:Anaerobiosis; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002374:Catalase / Q000378:metabolism; D016000:Cluster Analysis; D027081:DNA Gyrase / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005407:Flagella / Q000502:physiology; D006460:Hemolysin Proteins / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007194:India; D008124:Locomotion; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010088:Oxidoreductases / Q000378:metabolism; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D020592:Shewanella / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D012965:Sodium Chloride / Q000378:metabolism; D013696:Temperature
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D006460:Hemolysin Proteins; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride; D010088:Oxidoreductases; D002374:Catalase; D027081:DNA Gyrase