title: Expression profiles of genes within a subregion of chicken major histocompatibility complex B in spleen after Marek's disease virus infection.
abstract: Major histocompatibility complex has previously been shown to influence the resistance of chicken to Marek's disease virus (MDV). However, little is known about expression of other genes in the MHC-I and II pathway after MDV infection. This study aimed at investigating 8 immune-related genes in the MHC core region that affects host responses to MDV. Spleens of infected and age-matched uninfected chickens were removed at 4, 7, 14, 21, and 28 d postinfection for gene expression detection using real-time PCR. Different expression patterns of MHC-I and II pathway genes were observed in the spleen. In the MHC-I pathway, the expression of transporter of antigen protein 1 (TAP1), transporter of antigen protein 2 (TAP2), and transporter of antigen protein-binding protein (TAPBP) genes was significantly increased in the spleen of MDV-infected than that of uninfected chickens. It indicated that host antivirus responses were generated to enhance antigen presentation. However, MHC-II pathway genes showed contrary trends. Classical MHC-II β chain major gene (BLB2) and nonclassical class II genes [DM α chain gene (DMA), DM β chain gene-1 (DMB1), and DM β chain gene-2 (DMB2)] had consistent lower transcripts in spleens of MDV-infected than that of uninfected chickens, which reflected that MDV interfered with multiple components of the MHC-II pathway. Overall, expression of most genes in the MHC core region was altered; moreover, the genes in endogenous and exogenous antigen presentation pathways had different expression patterns in the spleen after MDV infection.
authors.affiliation: Department of Animal Genetics and Breeding, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
authors.forename: L
authors.initials: L
authors.lastname: Lian
authors.forename: L J
authors.initials: LJ
authors.lastname: Qu
authors.forename: J X
authors.initials: JX
authors.lastname: Zheng
authors.forename: C J
authors.initials: CJ
authors.lastname: Liu
authors.forename: Y P
authors.initials: YP
authors.lastname: Zhang
authors.forename: Y M
authors.initials: YM
authors.lastname: Chen
authors.forename: G Y
authors.initials: GY
authors.lastname: Xu
authors.forename: N
authors.initials: N
authors.lastname: Yang
mesh_terms: D000818:Animals; D002645:Chickens; D020869:Gene Expression Profiling / Q000662:veterinary*; D005786:Gene Expression Regulation / Q000276:immunology*; D008285:Major Histocompatibility Complex / Q000235:genetics / Q000502:physiology*; D025922:Mardivirus; D008380:Marek Disease / Q000378:metabolism*; D013154:Spleen / Q000378:metabolism*; D013997:Time Factors