[Experimental research on osteogenic abilities of new bone tissue engineering scaffolds by recombinant bone morphogenetic protein].
This research sought to asses the efficacity of a new type of tissue engineering bone developed by PDLLA/ PLA-PEG-PLA and BMP as a kind of bone graft substitute in the rabbit model of mandibular defects; 15 mm x 6 mm bilateral mandibular periosteum bone defects were made surgically in 20 New Zealand adult rabbits. The porous scaffolds impregnated with rhBMP-2 were used for the purpose, and the scaffolds without rhBMP-2 were used as control. The methods adopted in this research were: macroscopy, histomorphologic exam, X-ray exam, SEM micrography, computer-aided analysis and graphics. The experimental group was shown to have an earlier inception of bone forming. New bone formation was seen along the border of the original mandibular bone and in the middle. At 12 weeks after surgery,the defects were almost filled with new bone. In the control group, the defects could not be repaired in its entirety, and there was no new bone in the middle. The porous scaffold is a promising carrier for BMP. This kind of bone graft substitute can serve as an osteoconductive and osteoinductive matrix.
Li
Yi
Y
The First Affiliated Hospital of Guangghou UNI of Traditional Chinese Medicine, Stomatology, Guangzhou 510405, China. liyi0@163.com
Ran
Wei
W
Liu
Manzhen
M
Liu
Yanhui
Y
D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000008:administration & dosage*; D018786:Bone Substitutes / Q000378:metabolism*; D005260:Female; D020007:Implants, Experimental; D019344:Lactic Acid / Q000008:administration & dosage; D008297:Male; D008338:Mandibular Injuries / Q000601:surgery*; D010012:Osteogenesis; D011091:Polyesters / Q000008:administration & dosage; D011092:Polyethylene Glycols / Q000008:administration & dosage; D011108:Polymers / Q000008:administration & dosage; D011817:Rabbits; D011994:Recombinant Proteins / Q000008:administration & dosage; D023822:Tissue Engineering; D054457:Tissue Scaffolds
D055396:Bone Morphogenetic Protein 2; D018786:Bone Substitutes; D011091:Polyesters; D011108:Polymers; D011994:Recombinant Proteins; C121059:poly(lactide-co-trimethylene carbonate); D019344:Lactic Acid; D011092:Polyethylene Glycols; C033616:poly(lactide)