LmrS is a multidrug efflux pump of the major facilitator superfamily from Staphylococcus aureus.
A multidrug efflux pump designated LmrS (lincomycin resistance protein of Staphylococcus aureus), belonging to the major facilitator superfamily (MFS) of transporters, was cloned, and the role of LmrS in antimicrobial efflux was evaluated. The highest relative increase in MIC, 16-fold, was observed for linezolid and tetraphenylphosphonium chloride (TPCL), followed by an 8-fold increase for sodium dodecyl sulfate (SDS), trimethoprim, and chloramphenicol. LmrS has 14 predicted membrane-spanning domains and is homologous to putative lincomycin resistance proteins of Bacillus spp., Lactobacillus spp., and Listeria spp.
Floyd
Jody L
JL
Eastern New Mexico University, Department of Biology, Station 33, Portales, NM 88130, USA.
Smith
Kenneth P
KP
Kumar
Sanath H
SH
Floyd
Jared T
JT
Varela
Manuel F
MF
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D002701:Chloramphenicol / Q000494:pharmacology; D024901:Drug Resistance, Multiple, Bacterial; D004996:Ethidium / Q000494:pharmacology; D026901:Membrane Transport Proteins / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D009861:Onium Compounds / Q000494:pharmacology; D009943:Organophosphorus Compounds / Q000494:pharmacology; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D014295:Trimethoprim / Q000494:pharmacology; D014640:Vancomycin / Q000494:pharmacology
D001426:Bacterial Proteins; D026901:Membrane Transport Proteins; D009861:Onium Compounds; D009943:Organophosphorus Compounds; D012967:Sodium Dodecyl Sulfate; D002701:Chloramphenicol; D014640:Vancomycin; D014295:Trimethoprim; D004996:Ethidium; C013289:tetraphenylphosphonium