Simultaneous detection of multiple single-nucleotide polymorphisms by a simple membrane chip.
Technologies that screen multiple single-nucleotide polymorphisms (SNPs) could be very valuable in predicting patients' susceptibilities to diseases or responses to therapeutic interventions. In this study, we developed a chip that can accurately detect four SNPs at same time. This chip is cost-effective and user-friendly because it uses a detection protocol analogous to dot blotting and does not require sophisticated instruments. To establish this chip, we designed and blotted onto a nylon membrane SNP-specific oligonucleotide probes for human angiotensinogen, cholesteryl ester transfer protein, and apolipoprotein E. This chip detected the corresponding SNPs harbored within the angiotensinogen, cholesteryl ester transfer protein, and apolipoprotein E sequences from 20 donors. Importantly, the SNPs detected by our chip matched exactly with the direct sequencing results, thereby highlighting the accuracy of this chip. In conclusion, our chip is a robust tool for multiple SNP screening and holds the potential to future refinement in detecting diseases-associating genes in patients.
Yang
Yu-Hsiang
YH
Graduate Institute of Medical Genetics, Kaohsiung Medical University, Kaohsiung, Taiwan.
Li
Ruei-Nian
RN
Tzou
Shey-Cherng
SC
Wang
Jaw-Yuan
JY
Lee
Hsin-Pai
HP
Wang
Hsien-Chung
HC
Chen
Fang-Ming
FM
Wang
Yung-Hsin
YH
Hsieh
Ming-Chia
MC
Huang
Ming-Yii
MY
Tseng
Wei-Lung
WL
Lin
Shiu-Ru
SR
Cheng
Tian-Lu
TL
D000808:Angiotensinogen / Q000235:genetics; D001057:Apolipoproteins E / Q000235:genetics; D053480:Cholesterol Ester Transfer Proteins / Q000235:genetics; D003362:Cost-Benefit Analysis; D015342:DNA Probes; D005820:Genetic Testing / Q000379:methods; D005838:Genotype; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000191:economics / Q000379:methods; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA
D001057:Apolipoproteins E; C506642:CETP protein, human; D053480:Cholesterol Ester Transfer Proteins; D015342:DNA Probes; D000808:Angiotensinogen