Fiber-optic particle plasmon resonance sensor for detection of interleukin-1β in synovial fluids.
A facile and label-free biosensing method has been developed for determining an osteoarthritis concerned cytokine, interleukin-1β (IL-1β), in synovial fluids. The biosensing technique, fiber-optic particle plasmon resonance (FOPPR), is based on gold nanoparticles-modified optical fiber where the gold nanoparticle surface has been modified by a mixed self-assembled monolayer for further conjugation of anti-IL-1β antibody and minimization of nonspecific adsorption. Upon binding of IL-1β to anti-IL-1β on the gold nanoparticle surface, the absorbance of the gold nanoparticle layer on the optical fiber changes and the signal change is enhanced through multiple total internal reflections along the optical fiber. Results show that the detection of IL-1β in synovial fluid by this sensor agrees quantitatively with the clinically accepted enzyme-linked immunosorbent assay (ELISA) method but a much shorter analysis time is required (<10 min). The sensor response versus log concentration of IL-1β was linear (r=0.9947) over the concentration range of 0.050-10 ng/mL and a limit of detection (LOD) of 21 pg/mL (1.2 pM) was achieved. Such a LOD for IL-1β (17 kDa) represents a major advancement in the field of real-time monitoring of low molecular weight proteins in complex biological fluids.
Chiang
Chang-Yue
CY
Department of Chemistry and Biochemistry, National Chung Cheng University, Chiayi 62102, Taiwan.
Hsieh
Ming-Lung
ML
Huang
Kuo-Wei
KW
Chau
Lai-Kwan
LK
Chang
Chia-Ming
CM
Lyu
Shaw-Ruey
SR
D055910:Antibodies, Immobilized; D004797:Enzyme-Linked Immunosorbent Assay; D006046:Gold; D006801:Humans; D053583:Interleukin-1beta / Q000032:analysis* / Q000276:immunology / Q000592:standards; D053768:Metal Nanoparticles; D055100:Optical Fibers; D010003:Osteoarthritis / Q000276:immunology; D012015:Reference Standards; D020349:Surface Plasmon Resonance / Q000295:instrumentation* / Q000592:standards / Q000706:statistics & numerical data; D013582:Synovial Fluid / Q000737:chemistry* / Q000276:immunology*
D055910:Antibodies, Immobilized; D053583:Interleukin-1beta; D006046:Gold