title: Molecular characterization of the full-length coding sequence of the caprine laminin receptor gene (RPSA).
abstract: Scrapie is a prion disease in sheep and goats. Ribosomal protein SA (RPSA), also called 37 kDa laminin receptor precursor/67 kDa laminin receptor has been demonstrated to be a putative cell surface receptor for prion. To investigate the caprine RPSA, we cloned the full-length coding sequence of the gene of goat and submitted it to GenBank. The length of the open reading frame is 888 bp, encoding 295 amino acids. The putative amino acid sequence is highly similar to that of other mammals. The caprine amino acid sequence of RPSA is shown to be identical to the sequence of species susceptible to scrapie at positions 241, 272, and 291. The phylogenetic tree analysis revealed that the genetic distance between sheep and goat is the smallest. Moreover, RT-PCR results of 11 tissues indicated that RPSA mRNA is expressed in all selected caprine tissues.
authors.affiliation: Agricultural University of Hebei, Baoding, China.
authors.forename: Rongyan
authors.initials: R
authors.lastname: Zhou
authors.forename: Xianglong
authors.initials: X
authors.lastname: Li
authors.forename: Huiqin
authors.initials: H
authors.lastname: Zheng
authors.forename: Lanhui
authors.initials: L
authors.lastname: Li
authors.forename: Weiyao
authors.initials: W
authors.lastname: Quan
authors.forename: Zunan
authors.initials: Z
authors.lastname: Shen
authors.forename: Jun
authors.initials: J
authors.lastname: Tong
authors.forename: Qingfang
authors.initials: Q
authors.lastname: Yang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Fu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015511:Goat Diseases / Q000235:genetics / Q000821:virology; D006041:Goats / Q000235:genetics / Q000821:virology; D006801:Humans; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D010802:Phylogeny; D011110:Polymorphism, Genetic; D011328:Prions / Q000235:genetics / Q000378:metabolism; D054730:Protein Interaction Domains and Motifs / Q000235:genetics; D011498:Protein Precursors / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism*; D017474:Receptors, Laminin / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism*; D012269:Ribosomal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012608:Scrapie / Q000235:genetics / Q000378:metabolism / Q000821:virology; D016415:Sequence Alignment; D012756:Sheep / Q000235:genetics / Q000821:virology
chemical_list: D011328:Prions; D011498:Protein Precursors; C510624:RPSA protein, human; D017474:Receptors, Laminin; D012269:Ribosomal Proteins