[In vitro study for detecting the effects of Bazhen decoction on proliferation and activation of T lymphocytes].
This study was designed to detect the effects of Bazhen decoction on the proliferation and activation of T lymphocytes. T lymphocytes were isolated. The effects of Bazhen decoction on the prolifertion and activation of T lymphocytes, and on the secretion of IFN-gamma IL-2 from T lymphocytes, were detected by MTT, Flow cytometry and ELISA. Results showed that proliferation of T lymphocytes was promoted significantly by different concentration of Bazhen decoction; and after different time, the relationships of "the longer the time, the higher the concentration, and the more enhanced the proliferation" came to be apparent. After 72h, T lymphocytes were activated with different concentration of Bazhen decoction, the rate of CD69+ T cells increased signficantly, the secretion of IFN-gamma and IL-2 increased signficicantly, and the effect exhibited a dose-dependent manner. The results of this epxeriment indicated that Bazhen decoction could promote the proliferation and activation of T lymphocytes.
Liu
Xiaoxia
X
Medical College of Hebei University of Engineering, Handan 056029, China. givesunny@163.com
Wang
Xueling
X
Li
Zhenhong
Z
Chen
Yumin
Y
Chen
Jianhua
J
D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005434:Flow Cytometry; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects* / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*
D004365:Drugs, Chinese Herbal