title: Protein quantitation using mass spectrometry.
abstract: Mass spectrometry is a method of choice for quantifying low-abundance proteins and peptides in many biological studies. Here, we describe a range of computational aspects of protein and peptide quantitation, including methods for finding and integrating mass spectrometric peptide peaks, and detecting interference to obtain a robust measure of the amount of proteins present in samples.
authors.affiliation: Kimmel Center for Biology and Medicine at the Skirball Institute and Department of Pharmacology, New York University School of Medicine, New York, NY, USA.
authors.forename: Guoan
authors.initials: G
authors.lastname: Zhang
authors.forename: Beatrix M
authors.initials: BM
authors.lastname: Ueberheide
authors.forename: Sofia
authors.initials: S
authors.lastname: Waldemarson
authors.forename: Sunnie
authors.initials: S
authors.lastname: Myung
authors.forename: Kelly
authors.initials: K
authors.lastname: Molloy
authors.forename: Jan
authors.initials: J
authors.lastname: Eriksson
authors.forename: Brian T
authors.initials: BT
authors.lastname: Chait
authors.forename: Thomas A
authors.initials: TA
authors.lastname: Neubert
authors.forename: David
authors.initials: D
authors.lastname: Fenyö
mesh_terms: D000595:Amino Acid Sequence; D007554:Isotopes; D013058:Mass Spectrometry / Q000379:methods*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010455:Peptides / Q000032:analysis / Q000737:chemistry; D011506:Proteins / Q000032:analysis* / Q000737:chemistry
chemical_list: D007554:Isotopes; D010455:Peptides; D011506:Proteins