Inhibitory effects of heartwood extracts of Broussonetia kazinoki Sieb on the development of atopic dermatitis in NC/Nga mice.
We investigated the effects of a topically applied extract of the heartwood of Broussonetia kazinoki Sieb (B. kazinoki) on atopic dermatitis (AD)-like skin lesions induced by an extract of the house-dust mite Dermatophagoides farina in NC/Nga mice. We found that topically applied B. kazinoki extract suppressed the histological manifestations of AD-like skin lesions, and decreased the levels of plasma immunoglobulin E (IgE) and interleukin-4 (IL-4) in the mice. Moreover, B. kazinoki inhibited the induction of thymus-and-activation-regulated chemokine (TARC/CCL17), macrophage-derived chemokine (MDC/CCL22), and regulated-on-activation-normal T cell-expressed-and-secreted chemokine (RANTES/CCL5) in HaCaT cells activated by tumor necrosis factor-alpha (TNF-alpha) and interferon-gamma (IFN-gamma). In conclusion, our results suggest that B. kazinoki extract has therapeutic advantages in the treatment of AD.
Lee
Jun-Kyoung
JK
Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine, Daejeon, Republic of Korea.
Ha
Hyekyung
H
Lee
Ho-Young
HY
Park
Sang-Joon
SJ
Jeong
Seung-ll
SL
Choi
Young-Jae
YJ
Shin
Hyeun Kyoo
HK
D000287:Administration, Topical; D000818:Animals; D031623:Broussonetia / Q000737:chemistry*; D054414:Chemokine CCL17 / Q000032:analysis; D054422:Chemokine CCL22 / Q000032:analysis; D018946:Chemokine CCL5 / Q000032:analysis; D003876:Dermatitis, Atopic / Q000188:drug therapy*; D007073:Immunoglobulin E / Q000032:analysis; D007371:Interferon-gamma / Q000494:pharmacology; D015847:Interleukin-4 / Q000032:analysis; D051379:Mice; D008815:Mice, Inbred Strains; D008517:Phytotherapy; D010936:Plant Extracts / Q000627:therapeutic use*; D010946:Plants, Medicinal; D039981:Pyroglyphidae / Q000276:immunology / Q000472:pathogenicity; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
C499538:Ccl17 protein, mouse; C515483:Ccl22 protein, mouse; C503460:Ccl5 protein, mouse; D054414:Chemokine CCL17; D054422:Chemokine CCL22; D018946:Chemokine CCL5; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D015847:Interleukin-4; D007073:Immunoglobulin E; D007371:Interferon-gamma