Visualizing the functional diversification of CD8+ T cell responses in lymph nodes.
CD8(+) T cell responses generate effector cells endowed with distinct functional potentials but the contribution of early events in this process is unclear. Here, we have imaged T cells expressing a fluorescent reporter for the activation of the interferon-γ (IFN-γ) locus during priming in lymph nodes. We have demonstrated marked differences in the efficiency of gene activation during stable T cell-dentritic cell (DC) contacts, influenced in part by signal strength. Imaging the first cell division, we have demonstrated that heterogeneity in T cell functional potential was largely apparent as T cells initiated clonal expansion. Moreover, by analyzing the fate of single activated T cells ex vivo, we have provided evidence that these early differences resulted in clonal progenies with distinct functional properties. Thus, the early set of T cell-DC interactions in lymph nodes largely contribute to the heterogeneity of T cell responses through the generation of functionally divergent clonal progenies.
Beuneu
Hélène
H
Institut Pasteur, Unité des Dynamiques des Réponses Immunes, Département d'Immunologie, Paris, France.
Lemaître
Fabrice
F
Deguine
Jacques
J
Moreau
Hélène D
HD
Bouvier
Isabelle
I
Garcia
Zacarias
Z
Albert
Matthew L
ML
Bousso
Philippe
P
D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002450:Cell Communication; D003713:Dendritic Cells / Q000276:immunology; D006412:Hematopoietic Stem Cells / Q000502:physiology; D007371:Interferon-gamma / Q000235:genetics; D008198:Lymph Nodes / Q000276:immunology*; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D013601:T-Lymphocytes / Q000276:immunology
D007371:Interferon-gamma