title: Functionality of sortase A in Lactococcus lactis.
abstract: Lactococcus lactis IL1403 harbors a putative sortase A (SrtA) and 11 putative sortase substrates that carry the canonical LPXTG signature of such substrates. We report here on the functionality of SrtA to anchor five LPXTG substrates to the cell wall, thus suggesting that SrtA is the housekeeping sortase in L. lactis IL1403.
authors.affiliation: INRA, UMR1319 Micalis, Domaine de Vilvert, F-78352 Jouy-en-Josas, France.
authors.forename: Yakhya
authors.initials: Y
authors.lastname: Dieye
authors.forename: Virginie
authors.initials: V
authors.lastname: Oxaran
authors.forename: Florence
authors.initials: F
authors.lastname: Ledue-Clier
authors.forename: Walid
authors.initials: W
authors.lastname: Alkhalaf
authors.forename: Girbe
authors.initials: G
authors.lastname: Buist
authors.forename: Vincent
authors.initials: V
authors.lastname: Juillard
authors.forename: Chang Won
authors.initials: CW
authors.lastname: Lee
authors.forename: Jean-Christophe
authors.initials: JC
authors.lastname: Piard
mesh_terms: D019881:Aminoacyltransferases / Q000378:metabolism / Q000502:physiology*; D001422:Bacterial Adhesion / Q000235:genetics / Q000502:physiology; D001426:Bacterial Proteins / Q000378:metabolism / Q000502:physiology*; D002473:Cell Wall / Q000378:metabolism / Q000502:physiology; D003546:Cysteine Endopeptidases / Q000378:metabolism / Q000502:physiology*; D005798:Genes, Bacterial / Q000235:genetics; D013294:Lactococcus lactis / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D016297:Mutagenesis, Site-Directed; D021381:Protein Transport / Q000235:genetics; D020543:Proteome / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D020543:Proteome; D011993:Recombinant Fusion Proteins; D019881:Aminoacyltransferases; C120542:sortase A; D003546:Cysteine Endopeptidases