PEG-functionalized magnetic nanoparticles for drug delivery and magnetic resonance imaging applications.
OBJECTIVE
Polyethylene glycol (PEG) functionalized magnetic nanoparticles (MNPs) were tested as a drug carrier system, as a magnetic resonance imaging (MRI) agent, and for their ability to conjugate to an antibody.


METHODS
An iron oxide core coated with oleic acid (OA) and then with OA-PEG forms a water-dispersible MNP formulation. Hydrophobic doxorubicin partitions into the OA layer for sustained drug delivery. The T(1) and T(2) MRI contrast properties were determined in vitro and the circulation of the MNPs was measured in mouse carotid arteries. An N-hydroxysuccinimide group (NHS) on the OA-PEG-80 was used to conjugate the amine functional group on antibodies for active targeting in the human MCF-7 breast cancer cell line.


RESULTS
The optimized formulation had a mean hydrodynamic diameter of 184 nm with an ~8 nm iron-oxide core. The MNPs enhance the T(2) MRI contrast and have a long circulation time in vivo with 30% relative concentration 50 min post-injection. Doxorubicin-loaded MNPs showed sustained drug release and dose-dependent antiproliferative effects in vitro; the drug effect was enhanced with transferrin antibody-conjugated MNPs.


CONCLUSIONS
PEG-functionalized MNPs could be developed as a targeted drug delivery system and MRI contrast agent.
Yallapu
Murali Mohan
MM
Department of Biomedical Engineering/ND-20 Lerner Research Institute, Cleveland Clinic, 9500 Euclid Avenue, Cleveland, OH 44195, USA.
Foy
Susan P
SP
Jain
Tapan K
TK
Labhasetwar
Vinod
V
D000818:Animals; D002460:Cell Line; D045744:Cell Line, Tumor; D016503:Drug Delivery Systems; D006801:Humans; D008279:Magnetic Resonance Imaging; D008280:Magnetics; D051379:Mice; D053758:Nanoparticles; D011092:Polyethylene Glycols
D011092:Polyethylene Glycols