title: Pma1, a P-type proton ATPase, is a determinant of chronological life span in fission yeast.
abstract: Chronological life span is defined by how long a cell can survive in a non-dividing state. In yeast, it is measured by viability after entry into stationary phase. To date, some factors affecting chronological life span have been identified; however, the molecular details of how these factors regulate chronological life span have not yet been elucidated clearly. Because life span is a complicated phenomenon and is supposedly regulated by many factors, it is necessary to identify new factors affecting chronological life span to understand life span regulation. To this end, we have screened for long-lived mutants and identified Pma1, an essential P-type proton ATPase, as one of the determinants of chronological life span. We show that partial loss of Pma1 activity not only by mutations but also by treatment with the Pma1 inhibitory chemical vanadate resulted in the long-lived phenotype in Schizosaccharomyces pombe. These findings suggest a novel way to manipulate chronological life span by modulating Pma1 as a molecular target.
authors.affiliation: Laboratory of Molecular Microbiology, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa-ku, Nagoya 464-8601, Japan.
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Ito
authors.forename: Tomoko
authors.initials: T
authors.lastname: Oshiro
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Fujita
authors.forename: Sachiko
authors.initials: S
authors.lastname: Kubota
authors.forename: Chikako
authors.initials: C
authors.lastname: Naito
authors.forename: Hokuto
authors.initials: H
authors.lastname: Ohtsuka
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Murakami
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Aiba
mesh_terms: D004791:Enzyme Inhibitors / Q000494:pharmacology; D009154:Mutation; D006180:Proton-Translocating ATPases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D012568:Schizosaccharomyces / Q000201:enzymology* / Q000235:genetics; D029702:Schizosaccharomyces pombe Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D014638:Vanadates / Q000494:pharmacology
chemical_list: D004791:Enzyme Inhibitors; D029702:Schizosaccharomyces pombe Proteins; D014638:Vanadates; D006180:Proton-Translocating ATPases