title: The cumulus cell gene expression profile of oocytes with different nuclear maturity and potential for blastocyst formation.
abstract: OBJECTIVE
  Gene expression in human ART cumulus cell (CC) has been related to oocyte maturity and competence but requires further validation. Expression dynamics were investigated in CC of oocytes at different maturational stages and with different developmental competence in a standard in vivo mouse superovulation model.
  
  
  METHODS
  Quantitative PCR analysis of Has2, Vcan, Sdc4, Alcam, Grem1, Ptgs1 and Ptgs2 in CC collected at regular time intervals from 0 to 24 h post hCG injection.
  
  
  RESULTS
  Three expression patterns were observed each with strong regulation (4-230× differences). Immediately prior to ovulation CC of GVBD oocytes have 5× less Sdc4 and Ptgs1 and 5× more Ptgs2 when compared to the CC of freshly ovulated PB oocytes. When compared to the latter, the post-ovulatory aged PB oocytes had a 2× reduced blastocyst forming capacity and their CC expressed 2× more Sdc4 and 6× less Alcam.
  
  
  CONCLUSIONS
  Morphologically identical cumulus oocyte complexes with different developmental competence can be differentiated by CC gene expression.
authors.affiliation: Follicle Biology Laboratory, Vrije Universiteit Brussel (VUB), Brussels, Belgium. Tom.Adriaenssens@UZBrussel.be
authors.forename: Tom
authors.initials: T
authors.lastname: Adriaenssens
authors.forename: Ingrid
authors.initials: I
authors.lastname: Segers
authors.forename: Sandra
authors.initials: S
authors.lastname: Wathlet
authors.forename: Johan
authors.initials: J
authors.lastname: Smitz
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001755:Blastocyst / Q000166:cytology; D002467:Cell Nucleus; D054885:Cumulus Cells / Q000166:cytology* / Q000378:metabolism*; D046149:Embryo Culture Techniques; D047108:Embryonic Development; D005260:Female; D005307:Fertilization in Vitro; D020869:Gene Expression Profiling; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D023421:Models, Animal; D009865:Oocytes / Q000166:cytology* / Q000378:metabolism; D013480:Superovulation