Optimization of medium for enhancement of ε-poly-L-lysine production by Streptomyces sp. M-Z18 with glycerol as carbon source.
The goal of this study was to increase ε-Poly-L-Lysine (ε-PL) production by Streptomyces sp. M-Z18 through optimization of the culture medium. Glycerol, (NH4)2SO4 and K2HPO4 were the key factors in the fermentation according to the results obtained from the Plackett-Burman experimental design. The optimal concentration of the three factors was further investigated according to the central composite design and determined to be 59.48, 4.43 and -0.49 g/L, respectively. Under optimal conditions, a ε-PL concentration of 2.27±0.15 g/L was obtained. Compared with glucose, fermentation of glycerol afforded 2.8-fold and 6.3-fold the ε-PL titer and yield, respectively. Therefore, glycerol may be an efficient carbon source for ε-PL production by Streptomyces sp. M-Z18.
Chen
Xusheng
X
Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, 214122 Wuxi, Jiangsu, China.
Tang
Lei
L
Li
Shu
S
Liao
Lijuan
L
Zhang
Jianhua
J
Mao
Zhonggui
Z
D000704:Analysis of Variance; D019149:Bioreactors / Q000382:microbiology; D001709:Biotechnology / Q000379:methods*; D002244:Carbon / Q000494:pharmacology*; D003470:Culture Media / Q000494:pharmacology*; D005285:Fermentation / Q000187:drug effects; D005990:Glycerol / Q000494:pharmacology*; D011107:Polylysine / Q000096:biosynthesis*; D012044:Regression Analysis; D015203:Reproducibility of Results; D013302:Streptomyces / Q000187:drug effects* / Q000378:metabolism*
D003470:Culture Media; D011107:Polylysine; D002244:Carbon; D005990:Glycerol