title: Anti-inflammatory and gastroprotective properties of Hypericum richeri oil extracts.
abstract: Oil extracts of flowering tops of Hypericum richeri Vill. prepared in three different ways were evaluated for chemical composition, and anti-inflammatory and gastroprotective activities. An HPLC method was developed for determination of two dominant flavonoids, quercetin and I3,II8-biapigenin. The carrageenan-induced rat paw edema test was used for screening the anti-inflammatory activity, while indomethacin-induced rat gastric mucosa damage test was used for evaluation of gastroprotective activity. The oil extract prepared by maceration with 96% ethanol, followed by extraction with sunflower oil by heating on a water bath, exhibited the highest anti-inflammatory (38.4%) and gastroprotective activities (gastric damage score of 0.9). The same oil extract had the highest content of quercetin (49 microg/mL) and I3,II8-biapigenin (60 microg/mL). These results approve the usage of oil extracts of H. richeri as an anti-inflammatory and gastroprotective agent.
authors.affiliation: Institute for Medicinal Plant Research "Dr Josif Pancić", Tadeusa Kosćuska 1, 11000 Belgrade, Serbia. gzdunic@mocbilja.rs
authors.forename: Gordana
authors.initials: G
authors.lastname: Zdunić
authors.forename: Dejan
authors.initials: D
authors.lastname: Godevac
authors.forename: Marina
authors.initials: M
authors.lastname: Milenković
authors.forename: Katarina
authors.initials: K
authors.lastname: Savikin
authors.forename: Nebojsa
authors.initials: N
authors.lastname: Menković
authors.forename: Silvana
authors.initials: S
authors.lastname: Petrović
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D005419:Flavonoids / Q000032:analysis; D005753:Gastric Mucosa / Q000187:drug effects*; D020902:Hypericum / Q000737:chemistry*; D007213:Indomethacin / Q000633:toxicity; D008297:Male; D010936:Plant Extracts / Q000494:pharmacology*; D020011:Protective Agents / Q000494:pharmacology; D011794:Quercetin / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000893:Anti-Inflammatory Agents; D005419:Flavonoids; D010936:Plant Extracts; D020011:Protective Agents; D011794:Quercetin; D007213:Indomethacin