An immunohistochemical analysis of a rat model of proliferative vitreoretinopathy and a comparison of the expression of TGF-β and PDGF among the induction methods.
Proliferative vitreoretinopathy (PVR) is a serious complication of retinal detachment surgery or ocular trauma. Our previous study indicated that intravitreal co-injection of retinal pigmented epithelial (RPE)-J cells and platelet-rich plasma (PRP) (not RPE-J cells or PRP alone) in Wistar rat eyes can successfully induce a model of PVR. But which cells are involved in this process and why different induction methods, intravitreal injection of RPE-J cells or/and PRP, induced a different situation remain to be unknown. In this study, immunohistochemistry was performed to identify the main cell types involved in this process. The expression levels of transforming growth factor (TGF)-β2, platelet-derived growth factor (PDGF)-AA and PDGF-BB were tested using enzyme-linked immunosorbent assay (ELISA). The results showed that RPE cells, glial cells, fibroblasts and macrophages took part in the pathogenesis of this model. The expression levels and durations of TGF-β2 and PDGF-BB partially explained the different results induced by the different induction methods. This provides an experimental proof for attenuation of the experimental PVR by targeting at a specific cells or growth factor.
Zheng
Xiao-Zhi
XZ
Department of Ultrasound, The Fourth Affiliated Hospital of Nantong University, The First People's Hospital of Yancheng, Yancheng 224006, Jiangsu Province, P.R.China.
Du
Lian-Fang
LF
Wang
Hui-Ping
HP
D000818:Animals; D000077214:Becaplermin; D001792:Blood Platelets / Q000502:physiology; D017690:Cell Transplantation; D002478:Cells, Cultured; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D010982:Platelet-Derived Growth Factor / Q000096:biosynthesis*; D020574:Proto-Oncogene Proteins c-sis; D051381:Rats; D017208:Rats, Wistar; D012160:Retina / Q000473:pathology; D016212:Transforming Growth Factor beta / Q000096:biosynthesis*; D018630:Vitreoretinopathy, Proliferative / Q000139:chemically induced / Q000473:pathology*; D014822:Vitreous Body / Q000473:pathology / Q000502:physiology
D005904:Glial Fibrillary Acidic Protein; D010982:Platelet-Derived Growth Factor; D020574:Proto-Oncogene Proteins c-sis; D016212:Transforming Growth Factor beta; C075278:platelet-derived growth factor A; D000077214:Becaplermin