title: Aniline-induced tryptophan production and identification of indole derivatives from three purple bacteria.
abstract: Growth on aniline by three purple non-sulfur bacteria (Rhodospirillum rubrum ATCC 11170, Rhodobacter sphaeroides DSM 158, and Rubrivivax benzoatiliticus JA2) as nitrogen, or carbon source could not be demonstrated. However in its presence, production of indole derivatives was observed with all the strains tested. At least 14 chromatographically (HPLC) distinct peaks were observed at the absorption maxima of 275-280 nm from aniline induced cultures. Five major indoles were identified based on HPLC and LC-MS/MS analysis. While tryptophan was the major common metabolite for all the three aniline induced cultures, production of indole-3-acetic acid was observed with Rvi. benzoatilyticus JA2 alone, while indole-3-aldehyde was identified from Rvi. benzoatilyticus JA2 and Rba. sphaeroides DSM 158. Indole-3-ethanol was identified only from Rsp. rubrum ATCC 1170 and anthranilic acid was identified from Rba. sphaeroides DSM 158.
authors.affiliation: Department of Plant Sciences, School of Life Sciences, University of Hyderabad, P.O. Central University, Hyderabad 500 046, India.
authors.forename: Md
authors.initials: M
authors.lastname: Mujahid
authors.forename: Ch
authors.initials: Ch
authors.lastname: Sasikala
authors.forename: Ch V
authors.initials: ChV
authors.lastname: Ramana
mesh_terms: D000814:Aniline Compounds / Q000378:metabolism*; D020562:Betaproteobacteria / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D007210:Indoleacetic Acids / Q000302:isolation & purification; D007211:Indoles / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism*; D007220:Industrial Waste; D013058:Mass Spectrometry; D053858:Metabolic Networks and Pathways; D012242:Rhodobacter sphaeroides / Q000378:metabolism*; D012247:Rhodospirillum rubrum / Q000378:metabolism*; D053719:Tandem Mass Spectrometry; D014364:Tryptophan / Q000378:metabolism*; D062367:ortho-Aminobenzoates / Q000302:isolation & purification; D000438:Alcohols
chemical_list: D000814:Aniline Compounds; D007210:Indoleacetic Acids; D007211:Indoles; D007220:Industrial Waste; D014364:Tryptophan; D062367:ortho-Aminobenzoates; C031385:anthranilic acid; C005949:tryptophol; D000438:Alcohols; C030737:indoleacetic acid; C012381:indole-3-carbaldehyde; C023650:aniline