title: A novel technique to center the donor punch for descemet stripping endothelial keratoplasty.
abstract: OBJECTIVE
  This report describes a method to improve the accuracy of centration of the donor trephination for Descemet stripping endothelial keratoplasty.
  
  
  METHODS
  Using trypan blue, the donor corneal cap is outlined. The donor punch is centered on the actual cap rim rather than on a centration mark made either by the surgeon or by the eye bank preparing the precut tissue.
  
  
  RESULTS
  Using the described technique, eccentric donor trephination was reduced from 7.7% to 2.4%.
  
  
  CONCLUSIONS
  We have described a method to improve the centration of donor trephination and reduce the incidence of eccentric donor punching.
authors.affiliation: Baylor College of Medicine, Department of Ophthalmology, Baylor College of Medicine, Houston, TX 77030, USA. mhamill@bcm.tmc.edu
authors.forename: Marshall Bowes
authors.initials: MB
authors.lastname: Hamill
authors.forename: Kevin Shuo
authors.initials: KS
authors.lastname: Zhang
mesh_terms: D003316:Corneal Diseases / Q000601:surgery; D057111:Descemet Stripping Endothelial Keratoplasty / Q000379:methods*; D004728:Endothelium, Corneal / Q000637:transplantation*; D006801:Humans; D015203:Reproducibility of Results; D014019:Tissue Donors; D014343:Trypan Blue
chemical_list: D014343:Trypan Blue