Tissue-specific regulation of mouse microRNA genes in endoderm-derived tissues.
MicroRNAs fine-tune the activity of hundreds of protein-coding genes. The identification of tissue-specific microRNAs and their promoters has been constrained by the limited sensitivity of prior microRNA quantification methods. Here, we determine the entire microRNAome of three endoderm-derived tissues, liver, jejunum and pancreas, using ultra-high throughput sequencing. Although many microRNA genes are expressed at comparable levels, 162 microRNAs exhibited striking tissue-specificity. After mapping the putative promoters for these microRNA genes using H3K4me3 histone occupancy, we analyzed the regulatory modules of 63 microRNAs differentially expressed between liver and jejunum or pancreas. We determined that the same transcriptional regulatory mechanisms govern tissue-specific gene expression of both mRNA and microRNA encoding genes in mammals.
Gao
Yan
Y
Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA 19104-6145, USA.
Schug
Jonathan
J
McKenna
Lindsay B
LB
Le Lay
John
J
Kaestner
Klaus H
KH
Greenbaum
Linda E
LE
D000818:Animals; D001665:Binding Sites; D004707:Endoderm / Q000378:metabolism; D005786:Gene Expression Regulation; D007583:Jejunum / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D010179:Pancreas / Q000378:metabolism; D011401:Promoter Regions, Genetic; D014157:Transcription Factors / Q000378:metabolism; D024363:Transcription Initiation Site
D035683:MicroRNAs; D014157:Transcription Factors