A study of association between expression of hOGG1, VDAC1, HK-2 and cervical carcinoma.
BACKGROUND
Human 8-oguanine glycosylase 1(hOGG1), voltage-dependent anion channel 1(VDAC1), hexokinase 2(HK-2), represented the process of oxidative DNA damage, cell apoptosis and glycolysis, respectively. This study aims to explore the association between expression of hOGG1, VDAC1, HK-2 and cervical carcinoma.


METHODS
A case-control study was conducted. 65 cervical biopsy samples consist of 20 control and 45 cases. The expression of hOGG1, VDAC1 and HK-2 were examined with immunohistochemistry(IHC), immunolabeling was evaluated with stereological cell counts.


RESULTS
The data showed that the positive proportion of hOGG1 and HK-2 in the case group was higher than that of the control group (P < 0.05). Further, there was an increasing trend for the positive proportion and expression degree of hOGG1 and HK-2 from Control, Mild cervical carcinoma (MCC), Intermediate cervical carcinoma(ICC) to Severe cervical carcinoma(SCC) in order (P < 0.05). To VDAC1, the significant result was not obtained.


CONCLUSIONS
The results suggested that there was a close association between expression of hOGG1, HK-2 and cervical cancer. hOGG1 and HK-2 might play a key role at the early stage of cervical cancer, and the findings of hOGG1 and HK-2 should be considered as a significant biomarker at the early stage of cervical cancer.
Guo-Qing
Peng
P
Department of O & G, Xiangya Hospital Central-South University, Changsha, China. [corrected]
Yuan
Yang
Y
Cai-Gao
Zhong
Z
Hongling
Yin
Y
Gonghua
Hu
H
Yan
Tian
T
D014408:Biomarkers, Tumor / Q000032:analysis; D001706:Biopsy; D002277:Carcinoma / Q000737:chemistry* / Q000473:pathology; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D002681:China; D045647:DNA Glycosylases / Q000032:analysis*; D005260:Female; D006593:Hexokinase / Q000032:analysis*; D006801:Humans; D007150:Immunohistochemistry; D009367:Neoplasm Staging; D015203:Reproducibility of Results; D002583:Uterine Cervical Neoplasms / Q000737:chemistry* / Q000473:pathology; D050995:Voltage-Dependent Anion Channel 1 / Q000032:analysis*
D014408:Biomarkers, Tumor; C494762:VDAC1 protein, human; D050995:Voltage-Dependent Anion Channel 1; D006593:Hexokinase; D045647:DNA Glycosylases; C479058:oxoguanine glycosylase 1, human