Immunodominant T-cell responses to dengue virus NS3 are associated with DHF.
Dengue infections are increasing at an alarming rate in many tropical and subtropical countries, where epidemics can put health care systems under extreme pressure. The more severe infections lead to dengue hemorrhagic fever (DHF), which can be life threatening. A variety of viral and host factors have been associated with the severity of dengue infections. Because secondary dengue infection is more commonly associated with DHF than primary infections, the acquired immune response to dengue, both B cells and T cells have been implicated. In this study, we set out to study T-cell responses across the entire dengue virus proteome and to see whether these were related to disease severity in a cohort of dengue-infected children from Thailand. Robust responses were observed in most infected individuals against most viral proteins. Responses to NS3 were the most frequent, and there was a very strong association between the magnitude of the response and disease severity. Furthermore, in DHF, cytokine-high CD107a-negative cells predominated.
Duangchinda
Thaneeya
T
Division of Immunology and Inflammation, Department of Medicine, Faculty of Medicine, Hammersmith Hospital, Imperial College London, London W12 0NN, UK.
Dejnirattisai
Wanwisa
W
Vasanawathana
Sirijit
S
Limpitikul
Wannee
W
Tangthawornchaikul
Nattaya
N
Malasit
Prida
P
Mongkolsapaya
Juthathip
J
Screaton
Gavin
G
D003716:Dengue Virus / Q000201:enzymology / Q000276:immunology*; D015234:HLA-A Antigens / Q000276:immunology; D006801:Humans; D020543:Proteome; D012697:Serine Endopeptidases / Q000276:immunology*; D019595:Severe Dengue / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D000085242:Viral Proteases
D015234:HLA-A Antigens; D020543:Proteome; D012697:Serine Endopeptidases; C504449:NS3 protease, dengue virus; D000085242:Viral Proteases