Escherichia coli-based production of a tumor idiotype antibody fragment--tetanus toxin fragment C fusion protein vaccine for B cell lymphoma.
The unique immunoglobulin idiotype expressed on the surface of B lymphoma cells can be used as an effective antigen in tumor-specific vaccines when fused to immunostimulatory proteins and cytokines. A DNA vaccine encoding for an idiotype antibody single chain Fv (scFv) fragment fused to the Tetanus Toxin Fragment C (TTFrC) has been shown to induce protective anti-tumor responses. Protein-based strategies may be more desirable since they provide greater control over dosage, duration of exposure, and in vivo distribution of the vaccine. However, production of fusion protein vaccines containing complex disulfide bonded idiotype antibodies and antibody-derived fragments is challenging. We use an Escherichia coli-based cell-free protein synthesis platform as well as high-level expression of E. coli inclusion bodies followed by refolding for the rapid generation of an antibody fragment - TTFrC fusion protein vaccine. Vaccine proteins produced using both methods were shown to elicit anti-tumor humoral responses as well as protect from tumor challenge in an established B cell lymphoma mouse model. The development of technologies for the rapid production of effective patient-specific tumor idiotype-based fusion protein vaccines provides opportunities for clinical application.
Patel
Kedar G
KG
Department of Chemical Engineering, Stanford University, 381 North-South Mall, Stanford, CA 94305-5025, USA. kpatel@amyris.com
Ng
Patrick P
PP
Levy
Shoshana
S
Levy
Ronald
R
Swartz
James R
JR
D000818:Animals; D019496:Cancer Vaccines / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification / Q000627:therapeutic use; D004926:Escherichia coli / Q000235:genetics*; D005260:Female; D006801:Humans; D007114:Immunization; D007130:Immunoglobulin Idiotypes / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification / Q000627:therapeutic use; D016393:Lymphoma, B-Cell / Q000276:immunology / Q000517:prevention & control*; D051379:Mice; D010446:Peptide Fragments / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification / Q000627:therapeutic use; D017510:Protein Folding; D013744:Tetanus Toxin / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification / Q000627:therapeutic use; D019444:Vaccines, DNA / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification / Q000627:therapeutic use
D019496:Cancer Vaccines; D007130:Immunoglobulin Idiotypes; D010446:Peptide Fragments; D013744:Tetanus Toxin; D019444:Vaccines, DNA; C040635:tetanus toxin fragment C