[The effect of SenQi FuZheng injection on lung cell apoptosis following cardiopulmonary bypass in dogs].
OBJECTIVE
To study the effect and possible mechanism of SenQi FuZheng injection on lung cell apoptosis in dogs with ischemia reperfusion (I/R) following cardiopulmonary bypass (CPB).


METHODS
CPB dog models were established. The 12 mongrel dogs were randomly divided into two groups: CPB I/R group (control) and SenQi FuZheng injection group (therapy). The lung tissue samples of 1.0 cm3 from the same lobes of lung were obtained at 10 min before CPB (T1) and 60 (T2), 120 min (T3) after reperfusion. These samples were fixed and then embedded in paraffin imbedding, and cut into thin slices (5 microm). Streptavidin/peroxidase (SP) immunohistochemical method was employed to detect Caspase -3, Bcl-2, and Bax protein expressions.


RESULTS
The expressions of Caspase-3 and Bax protein in the tissues with SenQi FuZheng therapy were significantly lower at 60 and 120 min after reperfusion than the controls (P < 0.05). The expression of Bcl-2 protein and the Bcl-2/Bax ratio in the tissues with SenQi FuZheng therapy were significantly higher at 60 and 120 min after reperfusion than the controls (P < 0.05).


CONCLUSIONS
Administration of SenQi FuZheng injection in CPB dogs can inhibit apoptosis of alveolar cells by reducing the expressions of Caspase-3 and Bax protein and increasing the expression of Bcl-2 protein.
Li
Hong-Chen
HC
Department of Thoracic and Cardiovascular Surgery, Affiliated Hospital, Zunyi Medical College, Zunyi 563003, China.
Liang
Gui-You
GY
Liu
Da-Xing
DX
Liu
Bin
B
D055371:Acute Lung Injury / Q000209:etiology / Q000517:prevention & control; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002315:Cardiopulmonary Bypass / Q000009:adverse effects*; D053148:Caspase 3 / Q000378:metabolism; D004285:Dogs; D004365:Drugs, Chinese Herbal / Q000494:pharmacology* / Q000627:therapeutic use; D005260:Female; D008168:Lung / Q000473:pathology; D008297:Male; D008517:Phytotherapy; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D011897:Random Allocation; D015427:Reperfusion Injury / Q000517:prevention & control*; D051028:bcl-2-Associated X Protein / Q000378:metabolism
D004365:Drugs, Chinese Herbal; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; C521737:shenqi fuzheng; D053148:Caspase 3