title: TbRGG2 facilitates kinetoplastid RNA editing initiation and progression past intrinsic pause sites.
abstract: TbRGG2 is an essential kinetoplastid RNA editing accessory factor that acts specifically on pan-edited RNAs. To understand the mechanism of TbRGG2 action, we undertook an in-depth analysis of edited RNA populations in TbRGG2 knockdown cells and an in vitro examination of the biochemical activities of the protein. We demonstrate that TbRGG2 down-regulation more severely impacts editing at the 5' ends of pan-edited RNAs than at their 3' ends. The initiation of editing is reduced to some extent in TbRGG2 knockdown cells. In addition, TbRGG2 plays a post-initiation role as editing becomes stalled in TbRGG2-depleted cells, resulting in an overall decrease in the 3' to 5' progression of editing. Detailed analyses of edited RNAs from wild-type and TbRGG2-depleted cells reveal that TbRGG2 facilitates progression of editing past intrinsic pause sites that often correspond to the 3' ends of cognate guide RNAs (gRNAs). In addition, noncanonically edited junction regions are either absent or significantly shortened in TbRGG2-depleted cells, consistent with impaired gRNA transitions. Sequence analysis further suggests that TbRGG2 facilitates complete utilization of certain gRNAs. In vitro RNA annealing and in vivo RNA unwinding assays demonstrate that TbRGG2 can modulate RNA-RNA interactions. Collectively, these data are consistent with a model in which TbRGG2 facilitates initiation and 3' to 5' progression of editing through its ability to affect gRNA utilization, both during the transition between specific gRNAs and during usage of certain gRNAs.
authors.affiliation: Department of Microbiology and Immunology, University at Buffalo School of Medicine, Buffalo, New York 14214, USA.
authors.forename: Michelle L
authors.initials: ML
authors.lastname: Ammerman
authors.forename: Vladimir
authors.initials: V
authors.lastname: Presnyak
authors.forename: John C
authors.initials: JC
authors.lastname: Fisk
authors.forename: Bardees M
authors.initials: BM
authors.lastname: Foda
authors.forename: Laurie K
authors.initials: LK
authors.lastname: Read
mesh_terms: D001483:Base Sequence; D018386:Kinetochores / Q000378:metabolism*; D008969:Molecular Sequence Data; D018087:Plastids / Q000378:metabolism*; D015800:Protozoan Proteins / Q000378:metabolism*; D017393:RNA Editing; D016053:RNA, Protozoan / Q000235:genetics; D014346:Trypanosoma brucei brucei / Q000235:genetics* / Q000378:metabolism*
chemical_list: D015800:Protozoan Proteins; D016053:RNA, Protozoan