Interaction of hantavirus nucleocapsid protein with ribosomal protein S19.
Hantaviruses, members of the Bunyaviridae family, are emerging category A pathogens that initiate the translation of their capped mRNAs by a novel mechanism mediated by viral nucleocapsid protein (N). N specifically binds to the mRNA 5' m7G cap and 40S ribosomal subunit, a complex of 18S rRNA and multiple ribosomal proteins. Here, we show that N specifically interacts with the ribosomal protein S19 (RPS19), located at the head region of the 40S subunit. We suggest that this N-RPS19 interaction facilitates ribosome loading on capped mRNAs during N-mediated translation initiation.
Haque
Absarul
A
Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, Kansas 66103, USA.
Mir
Mohammad A
MA
D015153:Blotting, Western; D017931:DNA Primers / Q000235:genetics; D006476:Orthohantavirus / Q000378:metabolism / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D047468:Immunoprecipitation; D013058:Mass Spectrometry; D019590:Nucleocapsid Proteins / Q000378:metabolism*; D014176:Protein Biosynthesis / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism*; D012269:Ribosomal Proteins / Q000378:metabolism*
D017931:DNA Primers; D019590:Nucleocapsid Proteins; D012333:RNA, Messenger; D012269:Ribosomal Proteins; C040343:ribosomal protein S19