title: Microwave-assisted Kochetkov amination followed by permanent charge derivatization: a facile strategy for glycomics.
abstract: We report a simple and rapid microwave-assisted method for the preparation of oligosaccharide-glycosylamines, followed by labelling with tris(2,4,6-trimethoxyphenyl)phosphonium acetic acid N-hydroxysuccinimide ester. The facile strategy introduced a permanent charge at the reducing end of the oligosaccharide. In combination of MALDI-MS, the detection limit for maltoheptaose was as low as 2 fmol μL(-1).
authors.affiliation: Department of Systems Biology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.forename: Guisen
authors.initials: G
authors.lastname: Zhang
authors.forename: Kenneth
authors.initials: K
authors.lastname: Chan
authors.forename: Jianjun
authors.initials: J
authors.lastname: Li
mesh_terms: D000586:Amination; D054794:Glycomics; D008872:Microwaves; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization