title: Direct high-frequency stimulation of peri-implant rabbit bone: a pilot study.
abstract: OBJECTIVE
  This study aimed to evaluate the effect of direct high-frequency mechanical stimulation on the peri-implant tissue healing.
  
  
  METHODS
  A total of 48 custom-made 2-mm diameter titanium implants were inserted in the tibial epiphyses of 12 rabbits. Half of the implants were stimulated by direct vibration (60±10 Hz) immediately after insertion for 1 and 4 weeks, respectively. The other half served as controls. The samples were collected after the animals were sacrificed and were histologically processed into paraffin sections and stained with haematoxylin and eosin. The bone fraction was measured in an area of 50 and 400 µm around the implant. To rate significant differences a one-way analysis of variance was used with α set at 5%.
  
  
  RESULTS
  No significant difference in bone fraction was found between test and control groups. When the bone fractions of the 50 and 400 µm peri-implant regions were compared, a significantly larger bone fraction was found in the 50 µm peri-implant region for the 4-week stimulated group.
  
  
  CONCLUSIONS
  Histomorphometric analyses could not reveal a pronounced effect of direct immediate high-frequency implant loading.
authors.affiliation: Department of Prosthetic Dentistry, BIOMAT Research Cluster, School of Dentistry, Oral Pathology and Maxillofacial Surgery, Katholieke Universiteit Leuven, Leuven, Belgium.
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Zhang
authors.forename: Ignace
authors.initials: I
authors.lastname: Naert
authors.forename: Dorien
authors.initials: D
authors.lastname: Van Schoonhoven
authors.forename: Joke
authors.initials: J
authors.lastname: Duyck
mesh_terms: D000818:Animals; D002113:Calcification, Physiologic / Q000502:physiology; D015921:Dental Implants; D003764:Dental Materials / Q000737:chemistry; D004838:Epiphyses / Q000503:physiopathology / Q000601:surgery; D005260:Female; D010812:Physical Stimulation; D011817:Rabbits; D013977:Tibia / Q000503:physiopathology* / Q000601:surgery; D013997:Time Factors; D014025:Titanium / Q000737:chemistry; D014732:Vibration; D014945:Wound Healing / Q000502:physiology
chemical_list: D015921:Dental Implants; D003764:Dental Materials; D014025:Titanium