title: Conversion of viable but nonculturable Vibrio cholerae to the culturable state by co-culture with eukaryotic cells.
abstract: VBNC Vibrio cholerae O139 VC-280 obtained by incubation in 1% solution of artificial sea water IO at 4°C for 74 days converted to the culturable state when co-cultured with CHO cells. Other eukaryotic cell lines, including HT-29, Caco-2, T84, HeLa, and Intestine 407, also supported conversion of VBNC cells to the culturable state. Conversion of VBNC V. cholerae O1 N16961 and V. cholerae O139 VC-280/pG13 to the culturable state, under the same conditions, was also confirmed. When VBNC V. cholerae O139 VC-280 was incubated in 1% IO at 4°C for up to 91 days, the number of cells converted by co-culture with CHO cells declined with each additional day of incubation and after 91 days conversion was not observed.
authors.affiliation: Collaborative Research Center of Okayama University for Infectious Diseases in India, Kolkata, India.
authors.forename: Mitsutoshi
authors.initials: M
authors.lastname: Senoh
authors.forename: Jayeeta
authors.initials: J
authors.lastname: Ghosh-Banerjee
authors.forename: Thandavarayan
authors.initials: T
authors.lastname: Ramamurthy
authors.forename: Takashi
authors.initials: T
authors.lastname: Hamabata
authors.forename: Takashi
authors.initials: T
authors.lastname: Kurakawa
authors.forename: Makoto
authors.initials: M
authors.lastname: Takeda
authors.forename: Rita R
authors.initials: RR
authors.lastname: Colwell
authors.forename: G Balakrish
authors.initials: GB
authors.lastname: Nair
authors.forename: Yoshifumi
authors.initials: Y
authors.lastname: Takeda
mesh_terms: D000818:Animals; D002460:Cell Line; D018920:Coculture Techniques; D006224:Cricetinae; D003412:Cricetulus; D017077:Culture Media, Conditioned; D006801:Humans; D014734:Vibrio cholerae / Q000166:cytology / Q000254:growth & development*
chemical_list: D017077:Culture Media, Conditioned