title: Pro-coagulant activity of phenolic acids isolated from Blumea riparia.
abstract: The effects of extracts of the aerial part of Blumea riparia DC. and their phenolic acids on hemostasis were evaluated. The EtOAc fraction showed significantly reduced blood clotting time (CT) and tail bleeding time of transection (BT) of mice in vivo. This fraction contained vanillic acid (1), syringic acid (2), p-coumaric acid (3), caffeic acid (4), and protocatechuic acid (5). Compound 1 reduced prothrombin time (PT), and strengthened mice uterine contractions. Compound 3 reduced CT and the activated partial thromboplastin time (APTT). Compound 5 reduced CT and increased the frequency of mice uterine contraction in a dose-dependent manner. Compound 2 reduced APTT. Compound 4 remarkably strengthened uterine contraction. Taken together, these data suggest that compounds 1, 3, and 5 possess procoagulant activity which jointly synergize blood coagulation via different mechanisms.
authors.affiliation: Department of Chemistry and Chemical Engineering, Guangxi University, Nanning, Daxue Road, 88, 530004, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Huang
authors.forename: Cuiwu
authors.initials: C
authors.lastname: Lin
authors.forename: Aiyuan
authors.initials: A
authors.lastname: Li
authors.forename: Baoyao
authors.initials: B
authors.lastname: Wei
authors.forename: Jianwen
authors.initials: J
authors.lastname: Teng
authors.forename: Lue
authors.initials: L
authors.lastname: Li
mesh_terms: D000818:Animals; D019659:Asteraceae / Q000737:chemistry*; D003029:Coagulants / Q000494:pharmacology*; D005260:Female; D062385:Hydroxybenzoates / Q000494:pharmacology*; D051379:Mice; D010314:Partial Thromboplastin Time; D014590:Uterine Contraction / Q000187:drug effects; D014914:Whole Blood Coagulation Time
chemical_list: D003029:Coagulants; D062385:Hydroxybenzoates