Vascular effects of a sulfated polysaccharide from the red marine alga Solieria filiformis.
Anticoagulant and antithrombotic properties of sulfated-polysaccharides (SP) from marine algae are extensively exploited. However, reports on the vascular effects of SP from red algae are rare in the literature. The polysaccharide from Solieria filiformis (Sf-SP) was isolated by ion exchange chromatography, analyzed by agarose gel electrophoresis and tested in male Wistar rats. The inflammation studies were performed using the paw-edema model and the relaxant activity in isolated aorta pre-contracted with phenylephrine. The anticoagulant effect was evaluated by the test of partial thromboplastin activation time. The SP (1 mg/kg) was not anti-inflammatory, but induced acute edema with maximal activity at 30 min (0.35 +/- 0.04 mL) compared to controls (0.05 +/- 0.03 mL). Cumulative addition of Sf-SP in phenylephrine-contracted tissues produced relaxation with maximal inhibition of 69% (IC50 29.3 +/- 9.0 microg/mL) at 300 microg/mL in comparison to controls (0.51 +/- 0.09 g). Sf-SP also extended human plasma coagulation time by 2.1 times. These substances could be used as important tools for the study of vascular alterations.
Assreuy
Ana Maria S
AM
Instituto Superior de Ciências Biomédicas, Universidade Estadual do Ceará, Av. Paranjana 1700, 60.740-000, Fortaleza-CE-Brazil. anassreuy@gmail.com
Pontes
Grazielle C
GC
Rodrigues
Natália V F C
NV
Gomes
Daniel M
DM
Xavier
Paulo A
PA
Araujo
Glacio S
GS
Sampaio
Alexandre H
AH
Cavada
Benildo S
BS
Pereira
Maria G
MG
Farias
Wladimir R L
WR
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D000925:Anticoagulants / Q000494:pharmacology*; D008297:Male; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D000461:Rhodophyta / Q000737:chemistry*; D013431:Sulfates / Q000494:pharmacology; D014664:Vasodilation / Q000187:drug effects
D000893:Anti-Inflammatory Agents; D000925:Anticoagulants; D011134:Polysaccharides; D013431:Sulfates