An optimized immunoaffinity fluorescent method for natural product target elucidation.
Understanding the mode of action of small molecules is an integral facet of drug discovery. We report an optimized immunoaffinity fluorescent method that allows one to conduct parallel studies at both the cellular and molecular level using a single probe construct. Viability of the method has been evaluated analytically and applied using glycyrrhetic acid as a model.
Yu
Wei-Luen
WL
Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093-0358, USA.
Guizzunti
Gianni
G
Foley
Timothy L
TL
Burkart
Michael D
MD
La Clair
James J
JJ
D001688:Biological Products / Q000737:chemistry*; D055808:Drug Discovery; D006034:Glycyrrhetinic Acid / Q000737:chemistry*; D006035:Glycyrrhiza / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D007118:Immunoassay / Q000379:methods; D008954:Models, Biological; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D013050:Spectrometry, Fluorescence / Q000379:methods*
D001688:Biological Products; D006034:Glycyrrhetinic Acid