Biodegradation of carbendazim by a novel actinobacterium Rhodococcus jialingiae djl-6-2.
A novel actinobacterial strain Rhodococcus jialingiae djl-6-2 capable of using carbendazim (MBC) as the sole carbon and nitrogen source for growth was used to investigate its metabolism pathway of MBC. The HPLC and MS/MS analysis showed that MBC was hydrolyzed to 2-aminobenzimidazole (2-AB) and then converted to benzimidazole or 2-hydroxybenzimidazole (2-HB). The conversion of 2-AB was inhibited by NH(4)NO(3). The benzene ring of 2-HB was further opened through meta (extradiol) catechol cleavage. The inoculation of strain djl-6-2 to MBC-contaminated soil resulted in a higher degradation rate than noninoculated soil, which indicated the potential of strain djl-6-2 in bioremediation of MBC polluted soil.
Wang
Zhichun
Z
Key Laboratory for Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China.
Wang
Yangyang
Y
Gong
Fenfen
F
Zhang
Juan
J
Hong
Qing
Q
Li
Shunpeng
S
D001562:Benzimidazoles / Q000737:chemistry / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002219:Carbamates / Q000737:chemistry / Q000378:metabolism*; D005659:Fungicides, Industrial / Q000737:chemistry / Q000378:metabolism*; D012240:Rhodococcus / Q000378:metabolism*; D012989:Soil Pollutants / Q000737:chemistry / Q000378:metabolism*
D001562:Benzimidazoles; D002219:Carbamates; D005659:Fungicides, Industrial; D012989:Soil Pollutants; C006698:carbendazim