title: Enhancement of bone formation by BMP-7 transduced MSCs on biomimetic nano-hydroxyapatite/polyamide composite scaffolds in repair of mandibular defects.
abstract: This study was to evaluate enhanced bone formation by bone morphogenetic protein-7 (BMP-7) transduced MSCs on nano-hydroxyapatite/polyamide (n-HA/PA) composite scaffolds for bone tissue engineering in repair of mandibular defect. n-HA/PA scaffolds were prepared and rabbit MSCs were separated and expanded; and then infected with adenoviral-mediated BMP-7 in vitro. The MSCs-BMP-7 and MSCs were seeded on the porous scaffolds. Scaffold/MSCs-BMP-7 constructs and scaffold/MSCs constructs were implanted in the defects of rabbits' mandible as the experimental groups A (n = 18) and groups B (n = 18), respectively, the pure scaffolds were implanted as controls (group C, n = 18). Six animals were sacrificed at 4-, 8-, and 16-week postimplantation, respectively. Their mandibles were removed and processed for radiographic, biomechanical tests, histological, and histomorphometric analysis. Group A animals showed greater bone formation and earlier mineralization than group B at 4- and 8-week postimplantation and similarly group B more than group C. However, no difference was found among three groups at 16-week postimplantation. The results of this study suggest that BMP-7 transduced MSCs-n-HA/PA composite could significantly accelerate bone formation in the implant at early stage. BMP-7 mediated ex vivo gene transfer based on MSCs as seed cells, combined with porous n-HA/PA as scaffolds for bone tissue engineering might be an alternative or supplemental approach to repair the mandibular defects.
authors.affiliation: State Key Laboratory of Oral Diseases, Department of Oral and Maxillofacial Surgery, Sichuan University West China College of Stomatology, Chengdu, China.
authors.forename: Jihua
authors.initials: J
authors.lastname: Li
authors.forename: Yubao
authors.initials: Y
authors.lastname: Li
authors.forename: Sancheng
authors.initials: S
authors.lastname: Ma
authors.forename: Ying
authors.initials: Y
authors.lastname: Gao
authors.forename: Yi
authors.initials: Y
authors.lastname: Zuo
authors.forename: Jing
authors.initials: J
authors.lastname: Hu
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology; D001696:Biomechanical Phenomena / Q000187:drug effects; D040761:Biomimetic Materials / Q000494:pharmacology; D055419:Bone Morphogenetic Protein 7 / Q000235:genetics / Q000378:metabolism*; D018920:Coculture Techniques; D017886:Durapatite / Q000494:pharmacology*; D006801:Humans; D020007:Implants, Experimental; D008334:Mandible / Q000000981:diagnostic imaging / Q000187:drug effects / Q000473:pathology*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D049329:Nanostructures / Q000737:chemistry / Q000648:ultrastructure; D009757:Nylons / Q000494:pharmacology*; D010012:Osteogenesis / Q000187:drug effects*; D011817:Rabbits; D011859:Radiography; D023822:Tissue Engineering; D054457:Tissue Scaffolds; D014161:Transduction, Genetic; D014162:Transfection; D014945:Wound Healing / Q000187:drug effects
chemical_list: D001672:Biocompatible Materials; D055419:Bone Morphogenetic Protein 7; D009757:Nylons; D017886:Durapatite