A high throughput micro-array system of polymer surfaces for the manipulation of primary pancreatic islet cells.
We developed a high throughput micro-arrayed polymer system for the study of polymer surfaces for islet cell culture. A micro-arrayed library with 496 different polymers was synthesized and used to examine attachment and insulin expression of islet cells. While most polymers were not supportive, several related polymers were identified as suitable ("hit's"). The "hit" arrays composed of "hit" polymers with 36 replicates were fabricated to confirm their capacities to support the attachment of islet cells, and these capacities were further validated in large surfaces. Notably, the attachment of islet cells on these synthetic polymeric films has been found to be as supportive as 804G supernatant coated tissue culture polystyrene dishes, one of the most extensively used substrates for the islet cell attachment. Interestingly, the polymeric surfaces optimal for a different cell type, hES derived cells, were distinct, highlighting the utility of these approaches for identifying cell type specific surfaces.
Mei
Ying
Y
Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA.
Hollister-Lock
Jennifer
J
Bogatyrev
Said R
SR
Cho
Seung-Woo
SW
Weir
Gordon C
GC
Langer
Robert
R
Anderson
Daniel G
DG
D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D016326:Extracellular Matrix Proteins / Q000494:pharmacology; D057166:High-Throughput Screening Assays / Q000379:methods*; D006801:Humans; D007515:Islets of Langerhans / Q000166:cytology* / Q000187:drug effects*; D046228:Microarray Analysis / Q000379:methods*; D011108:Polymers / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013499:Surface Properties / Q000187:drug effects
D001672:Biocompatible Materials; D016326:Extracellular Matrix Proteins; D011108:Polymers