title: Hepatoprotective treatment attenuates oxidative damages induced by carbon tetrachloride in rats.
abstract: The present study evaluated the hepatoprotective effect of an N-acetyl dl-methionine+choline chloride+caffeine+thiamine hydrochloride+nicotinamide+pyridoxine hydrochloride compound at doses of 0.2, 0.6 and 1.0 mL/kg of b.w., and the assessment was done by the investigation of serum-enzymatic activity, metabolic functions of the liver and histophatological changes in female Wistar rats, which were subjected to experimental intoxication with CCl(4). One hundred and nineteen rats were randomly distributed into 17 groups, performing five different treatments, being evaluated seven animals per treatment in four periods: 2, 4, 6 and 8 days after CCl(4)-induced intoxication. Treated rats with the hepatoprotective medicine (HM) presented a significant reduction in infiltration of inflammatory cells, steatosis, necrosis and liver congestion when compared to non-treated rats (control). Beside these results, the treatment showed a positive effect on circulatory alterations in the intoxicated animals, with reduction of spleen and renal congestion, as well as, promotion of a significant improvement in ALT, AST, LDH, ALP, GGT enzymatic serum activity reduction and in recovering liver function regarding the metabolism of urea, triglycerides and glucose. These findings indicate therapeutic usefulness of the compound when administered at dose 0.6 and 1.0 mL/kg of b.w. in female Wistar rats.
authors.affiliation: College of Veterinary Medicine, Laboratory of Pharmacology and Clinical Pathology, Camilo Castelo Branco University (Unicastelo), Descalvado/SP, Brazil. maabelo@hotmail.com
authors.forename: Marco Antonio
authors.initials: MA
authors.lastname: de Andrade Belo
authors.forename: Vando Edésio
authors.initials: VE
authors.lastname: Soares
authors.forename: Luciano Melo
authors.initials: LM
authors.lastname: de Souza
authors.forename: Márcia Ferreira
authors.initials: MF
authors.lastname: da Rosa Sobreira
authors.forename: Daniela Miyasaka Silveira
authors.initials: DM
authors.lastname: Cassol
authors.forename: Sandra Barioni
authors.initials: SB
authors.lastname: Toma
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002110:Caffeine / Q000008:administration & dosage*; D002251:Carbon Tetrachloride / Q000633:toxicity*; D056486:Chemical and Drug Induced Liver Injury / Q000473:pathology / Q000517:prevention & control*; D005260:Female; D008715:Methionine / Q000008:administration & dosage*; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D014803:Vitamin B Complex / Q000008:administration & dosage*
chemical_list: D000975:Antioxidants; D014803:Vitamin B Complex; D002110:Caffeine; D008715:Methionine; D002251:Carbon Tetrachloride