Label-free impedimetric sensor for a ribonucleic acid oligomer specific to hepatitis C virus at a self-assembled monolayer-covered electrode.
A ribonucleic acid (RNA) sensor based on hybridization of its peptide nucleic acid (PNA) molecule with a target RNA oligomer of the internal ribosome entry site sequence specific to the hepatitis C virus (HCV) and the electrochemical impedance detection is described. This RNA is one of the most conservative molecules of the whole HCV RNA genome. The ammonium ion terminated PNA molecule was immobilized via its host-guest interactions with the diaza crown ring of 3-thiophene-acetamide-diaza-18-crown-6 synthesized by a simple two-step method, which forms a well-defined self-assembled monolayer (SAM) on gold. Hybridization events of the probe PNA with the target RNA were monitored by measuring charge-transfer resistances for the Fe(CN)(6)(3-/4-) redox probe using Fourier transform electrochemical impedance spectroscopy. The ratio of the resistances of the SAM-covered electrode measured before and after hybridization increased linearly with log[RNA] in the rat liver lysate with a detection limit of about 23 pM.
Park
Jin-Young
JY
Department of Chemical Engineering, Pohang University of Science and Technology, Pohang 790-784, Gyeongbuk, Korea.
Lee
Yoon-suk
YS
Chang
Byoung-Yong
BY
Kim
Byeang Hyean
BH
Jeon
Sangmin
S
Park
Su-Moon
SM
D000818:Animals; D001483:Base Sequence; D015374:Biosensing Techniques / Q000379:methods*; D043844:Crown Ethers / Q000737:chemistry; D058266:Dielectric Spectroscopy / Q000379:methods*; D004566:Electrodes; D005292:Ferricyanides / Q000737:chemistry; D005583:Fourier Analysis; D006046:Gold / Q000737:chemistry; D016174:Hepacivirus / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D010084:Oxidation-Reduction; D020135:Peptide Nucleic Acids / Q000737:chemistry*; D012313:RNA / Q000737:chemistry*; D051381:Rats
D043844:Crown Ethers; D005292:Ferricyanides; D020135:Peptide Nucleic Acids; C007931:hexacyanoferrate III; D012313:RNA; C015762:18-crown-6; D006046:Gold