Basic study of retinal stem/progenitor cell separation from mouse iris tissue.
We described the possibility of retinal regeneration using a novel and efficient technique for culturing and separating retinal stem/progenitor cells from iris tissue. Immunohistochemical staining of adult agouti mouse iris tissue revealed the presence of nestin/low-affinity neurotrophin receptor p75 (p75(NTR))-positive cells on the endothelium camerae anterioris side. Cultured mouse iris-derived cells contained little or no melanin and were found to be positive for nestin. Most nestin-positive cells were analyzed for the coexpression of p75(NTR) as a cell membrane protein. When the p75(NTR) was used as a marker to sort the cells, we obtained a dense population of nestin-positive cells. Furthermore, the nestin/p75(NTR)-positive cells were able to differentiate into neural retina cells. Thus, this culture and separation technique is useful for obtaining retinal stem/progenitor cells from adult mouse iris tissue and for the efficient production of neural retina cells.
Yamamoto
Naoki
N
Laboratory of Molecular Biology & Histochemistry, Fujita Health University Joint Research Laboratory, 1-98 Dengakugakubo, Kutsukake-cho, Toyoake, Aichi 470-1192, Japan. naokiy@fujita-hu.ac.jp
Tanikawa
Atsuhiro
A
Horiguchi
Masayuki
M
D000068816:Adapalene; D054366:Agouti Signaling Protein / Q000032:analysis / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D007381:Intermediate Filament Proteins / Q000032:analysis / Q000378:metabolism; D007498:Iris / Q000166:cytology*; D008297:Male; D051379:Mice; D009281:Naphthalenes / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000378:metabolism; D064231:Nestin; D017475:Receptors, Nerve Growth Factor / Q000032:analysis / Q000378:metabolism*; D012038:Regeneration; D012160:Retina / Q000166:cytology / Q000502:physiology*; D013234:Stem Cells / Q000166:cytology / Q000502:physiology*
D054366:Agouti Signaling Protein; D007381:Intermediate Filament Proteins; D009281:Naphthalenes; D009419:Nerve Tissue Proteins; C577485:Nes protein, mouse; D064231:Nestin; D017475:Receptors, Nerve Growth Factor; C517634:Ngfr protein, mouse; C515183:a protein, mouse; D000068816:Adapalene