Antitumor activity of satraplatin in cisplatin-resistant oral squamous cell carcinoma cells.
BACKGROUND
The aim of the current study was to identify the antitumor activity of satraplatin in paired cisplatin (CDDP)-resistant oral squamous cell carcinoma (OSCC) cell line and its parental cell line.


METHODS
CDDP-resistant (KB-R) cells and parental cells (KB) pair were used. Viability was assessed using the MTT and clonogenic assay. Real-time polymerase chain reaction (PCR), glutathione (GSH) assay, and flow cytometric analysis were used for further assessment.


RESULTS
KB-R cells did not show cross-resistance to satraplatin. The expression status of almost all transporters was upregulated in the KB-R cells. There was no difference in the GSH levels between the KB and KB-R cells. Flow cytometric analysis indicated that with satraplatin the G2/M phase was arrested in the KB-R cells. KB-R cells contain enriched side population cells.


CONCLUSIONS
These data suggested that satraplatin has antitumor activity against the CDDP-resistant OSCC cells. The mechanism of cross-resistance to platinum agents seems to be multifactorial.
Yamano
Yukio
Y
Department of Clinical Molecular Biology, Graduate School of Medicine, Chiba University, Chiba, Japan.
Shiiba
Masashi
M
Negoro
Kenji
K
Nakatani
Ken
K
Kasamatsu
Atsushi
A
Yamatoji
Masanobu
M
Sakuma
Kentaro
K
Ogoshi
Kenji
K
Iyoda
Manabu
M
Shinozuka
Keiji
K
Yokoe
Hidetaka
H
Wada
Takeshi
T
Fujita
Shigeyuki
S
Iwasawa
Shunichiro
S
Takiguchi
Yuichi
Y
Tanzawa
Hideki
H
Uzawa
Katsuhiro
K
D000970:Antineoplastic Agents / Q000494:pharmacology; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy* / Q000473:pathology; D049109:Cell Proliferation / Q000187:drug effects*; D002945:Cisplatin / Q000494:pharmacology*; D016001:Confidence Intervals; D019008:Drug Resistance, Neoplasm; D005434:Flow Cytometry; D006801:Humans; D009062:Mouth Neoplasms / Q000188:drug therapy* / Q000473:pathology; D009944:Organoplatinum Compounds / Q000494:pharmacology*; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D018709:Statistics, Nonparametric; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000970:Antineoplastic Agents; D009944:Organoplatinum Compounds; D012333:RNA, Messenger; C081294:satraplatin; D002945:Cisplatin