The effect of magnetic nanoparticles of Fe(3)O(4) on immune function in normal ICR mice.
We investigated the effect of magnetic nanoparticles of Fe(3)O(4) (Fe(3)O(4)-MNPs) on the mice immune system. Imprinting control region (ICR) mice were assigned randomly into four groups and treated with normal saline or low, medium, or high doses of Fe(3)O(4)-MNPs, respectively. After intravenous administration of Fe(3)O(4)-MNPs for 72 hours, the peripheral T cells and the induction of primary immune responses in mice were investigated by flow cytometry and determined using enzyme-linked immunosorbent assay, respectively. The results showed that the ratio of spleen to body weight was not different between the experimental groups and control group (P > 0.05). The lymphocyte transformation rates in the suspension of spleen were higher in low-dose group than those in the control group (P < 0.05), while the proliferation of splenocytes was low in the medium and high groups when compared to the control group (P < 0.05). In peripheral blood, both the proportions of subset CD4(+) and CD8(+) T lymphocytes in the low-dose group were higher than those in the control group, whereas there was no difference in the number of CD4(+) T cells between the medium- and low-dose groups. Interestingly, the Fe(3)O(4)-MNPs enhanced the production of interleukin-2 (IL-2), interferon-γ, and IL-10 but did not affect the production of IL-4 in peripheral blood. It is concluded that Fe(3)O(4)-MNPs could influence immune functions of normal ICR mice in a dose-dependent manner.
Chen
Bao-An
BA
Department of Hematology, Zhongda Hospital, Clinical Medical School, Southeast University, Nanjing, People's Republic of China. cba8888@hotmail.com
Jin
Nan
N
Wang
Jun
J
Ding
Jiahua
J
Gao
Chong
C
Cheng
Jian
J
Xia
Guohua
G
Gao
Feng
F
Zhou
Yin
Y
Chen
Yue
Y
Zhou
Guina
G
Li
Xiaomao
X
Zhang
Yu
Y
Tang
Men
M
Wang
Xuemei
X
D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000187:drug effects / Q000276:immunology; D016207:Cytokines / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D005260:Female; D007109:Immunity / Q000187:drug effects*; D008213:Lymphocyte Activation / Q000187:drug effects; D058185:Magnetite Nanoparticles / Q000008:administration & dosage* / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D046529:Microscopy, Electron, Transmission; D050997:Nanomedicine; D010316:Particle Size; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000276:immunology
D016207:Cytokines; D058185:Magnetite Nanoparticles