title: Platelets enhance lymphocyte adhesion and infiltration into arterial thrombus.
abstract: Lymphocytes are present in atherosclerotic lesion. We hypothesise that platelets may facilitate lymphocyte infiltration into the arterial wall. Reconstituted human blood or whole blood was perfused through a collagen-coated parallel-plate flow chamber at different shear rates. Adhered platelets markedly enhanced lymphocyte adhesion that increased lymphocyte deposition from 10 ± 3 cells/mm2 of platelet-depleted blood to 38 ± 11 cells/mm2 of platelet-containing blood at the arterial shear rate of 500 s-1. Platelet-dependent lymphocyte adhesion was inhibited by P-selectin, CD40L, and GPIIb/IIIa-blocking agents, suggesting the involvement of multiple adhesion molecules in this heterotypic interaction. Lymphocyte deposition was more marked among T cells, and seen in both small and large cells. B and natural killer cell adhesion was, however, mainly seen in small cells. Platelet-conjugation facilitated lymphocyte adhesion, as suggested by the selective deposition of platelet-conjugated lymphocytes. In a mouse model of arterial thrombosis, FeCl3-induced thrombus formation markedly enhanced lymphocyte adhesion and infiltration into platelet thrombi, which was abolished by GPIIb/IIIa inhibition. In conclusion, platelets support lymphocyte adhesion under arterial flow conditions, which is selective among T cells and involves multiple adhesion molecules. Our data imply that platelets may facilitate the recruitment of circulating lymphocytes at the arterial injured sites.
authors.affiliation: Department of Pathology and Pathophysiology, Zhejiang University School of Medicine, Hangzhou, China.
authors.forename: Hu
authors.initials: H
authors.lastname: Hu
authors.forename: Linjing
authors.initials: L
authors.lastname: Zhu
authors.forename: Zhangsen
authors.initials: Z
authors.lastname: Huang
authors.forename: Qiushang
authors.initials: Q
authors.lastname: Ji
authors.forename: Madhumita
authors.initials: M
authors.lastname: Chatterjee
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Nailin
authors.initials: N
authors.lastname: Li
mesh_terms: D000328:Adult; D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D001160:Arterioles / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology; D001792:Blood Platelets / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D023201:CD40 Ligand / Q000037:antagonists & inhibitors / Q000378:metabolism; D002712:Chlorides; D003094:Collagen / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D005290:Ferric Compounds; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology; D036904:Leukocyte Rolling / Q000187:drug effects; D008297:Male; D008643:Mesentery / Q000098:blood supply*; D051379:Mice; D008810:Mice, Inbred C57BL; D008875:Middle Aged; D019007:P-Selectin / Q000037:antagonists & inhibitors / Q000378:metabolism; D010973:Platelet Adhesiveness / Q000187:drug effects; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology; D019039:Platelet Glycoprotein GPIIb-IIIa Complex / Q000037:antagonists & inhibitors / Q000378:metabolism; D013314:Stress, Mechanical; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D013927:Thrombosis / Q000097:blood / Q000139:chemically induced / Q000276:immunology*; D013997:Time Factors; D055815:Young Adult
chemical_list: D000911:Antibodies, Monoclonal; D002712:Chlorides; D005290:Ferric Compounds; D019007:P-Selectin; D010975:Platelet Aggregation Inhibitors; D019039:Platelet Glycoprotein GPIIb-IIIa Complex; D023201:CD40 Ligand; D003094:Collagen; C024555:ferric chloride