Fluorinase mediated chemoenzymatic synthesis of [(18)F]-fluoroacetate.
A novel preparation of sodium [(18)F]-fluoroacetate is described where 5'-[(18)F]-fluoro-5'-deoxyadenosine is generated by a fluorinase catalysed reaction of S-adenosyl-l-methionine (SAM) with no carrier added [(18)F]-fluoride and then oxidation to [(18)F]-fluoroacetate by a Kuhn-Roth oxidative degradation.
Li
Xiang-Guo
XG
University of St Andrews, School of Chemistry and Centre for Biomolecular Sciences, North Haugh, St Andrews, Fife, KY16 9ST, UK. xiali@utu.fi
Domarkas
Juozas
J
O'Hagan
David
D
D001426:Bacterial Proteins / Q000378:metabolism*; D001711:Biotransformation; D005463:Fluoroacetates / Q000138:chemical synthesis* / Q000378:metabolism*; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000378:metabolism*; D013302:Streptomyces / Q000201:enzymology; D013696:Temperature
D001426:Bacterial Proteins; D005463:Fluoroacetates; C014815:fluoroacetic acid; D010088:Oxidoreductases; C495766:fluorinase