Lipopolysaccharide from Burkholderia thailandensis E264 provides protection in a murine model of melioidosis.
Burkholderia thailandensis is a less virulent close relative of Burkholderia pseudomallei, a CDC category B biothreat agent. We have previously shown that lipopolysaccharide (LPS) extracted from B. pseudomallei can provide protection against a lethal challenge of B. pseudomallei in a mouse model of melioidosis. Sugar analysis on LPS from B. thailandensis strain E264 confirmed that this polysaccharide has a similar structure to LPS from B. pseudomallei. Mice were immunised with LPS from B. thailandensis or B. pseudomallei and challenged with a lethal dose of B. pseudomallei strain K96243. Similar protection levels were observed when either LPS was used as the immunogen. This data suggests that B. thailandensis LPS has the potential to be used as part of a subunit based vaccine against pathogenic B. pseudomallei.
Ngugi
Sarah A
SA
Dstl Porton Down, Salisbury, Wiltshire SP4 0JQ, UK.
Ventura
Valeria V
VV
Qazi
Omar
O
Harding
Sarah V
SV
Kitto
G Barrie
GB
Estes
D Mark
DM
Dell
Anne
A
Titball
Richard W
RW
Atkins
Timothy P
TP
Brown
Katherine A
KA
Hitchen
Paul G
PG
Prior
Joann L
JL
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000276:immunology / Q000302:isolation & purification; D001428:Bacterial Vaccines / Q000276:immunology; D019117:Burkholderia / Q000276:immunology / Q000472:pathogenicity*; D016957:Burkholderia pseudomallei / Q000276:immunology / Q000472:pathogenicity*; D004195:Disease Models, Animal; D005260:Female; D008070:Lipopolysaccharides / Q000276:immunology* / Q000302:isolation & purification; D008554:Melioidosis / Q000276:immunology / Q000517:prevention & control*; D051379:Mice; D008807:Mice, Inbred BALB C
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001428:Bacterial Vaccines; D008070:Lipopolysaccharides