title: The prevalence and distribution of Chlamydia trachomatis genotypes among sexually transmitted disease clinic patients in Guangzhou, China, 2005-2008.
abstract: This study was designed to determine the prevalence and distribution of Chlamydia trachomatis genotypes from clinical specimens in Guangzhou, China, obtained in the period 2005-2008. One hundred and ninety-four urogenital C. trachomatis samples were collected from sexually transmitted disease clinic patients, and the VS1-VS2 of OmpA gene was amplified by nested PCR and sequenced using an ABI-prism 3730 sequencer. Clinical C. trachomatis strains were genotyped and analyzed for a mutation with respect to the reference VS1-VS2 sequence. VS1-VS2 fragments with 453 bp were amplified from 194 clinical samples. Upon alignment with the sequences of the reference strains, 189 strains with discernible sequences were typed into 9 genotypes, while 5 with ambiguous sequences were considered to be mixed-serovar samples. The most prevalent genotypes were E (50, 26%), F (46, 24%), J (35, 19%), and D (24, 13%). There was no significant difference in the distribution of any of the genotypes detected during the study period, except for genotype K (P<0.01). A total of 16 (8%, 16/189) genetic variants of the OmpA VS1-VS2 of the reference strains were identified. Mutations occurred frequently for genotypes D (2/24, 8%), E (6/50, 12%), F (2/46, 4%), G (1/8, 13%), H (1/12, 8%), and K (4/11, 36%), with most of these being sense mutations that may result in amino acid substitution. Sequencing the OmpA VS1-VS2 enabled the genotype and sequence variations within each genotype to be analyzed. Genotypes E, F, J, and D continued to dominate among urogenital C. trachomatis, whereas genotype K increased significantly in Guangzhou between 2005 and 2008.
authors.affiliation: Guangdong Provincial Center for Skin Diseases and STI Control and Prevention, Guangzhou, China. bin_yanggz@yahoo.com.cn
authors.forename: Bin
authors.initials: B
authors.lastname: Yang
authors.forename: He-Ping
authors.initials: HP
authors.lastname: Zheng
authors.forename: Zhan-Qin
authors.initials: ZQ
authors.lastname: Feng
authors.forename: Yao-Hua
authors.initials: YH
authors.lastname: Xue
authors.forename: Xing-Zhong
authors.initials: XZ
authors.lastname: Wu
authors.forename: Jin-Mei
authors.initials: JM
authors.lastname: Huang
authors.forename: Xiu-Juan
authors.initials: XJ
authors.lastname: Xue
authors.forename: Han-Ning
authors.initials: HN
authors.lastname: Jiang
mesh_terms: D000554:Ambulatory Care Facilities; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D016009:Chi-Square Distribution; D002681:China / Q000453:epidemiology; D002690:Chlamydia Infections / Q000453:epidemiology* / Q000382:microbiology*; D002692:Chlamydia trachomatis / Q000235:genetics*; D003430:Cross-Sectional Studies; D004252:DNA Mutational Analysis / Q000379:methods; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D009154:Mutation; D016133:Polymerase Chain Reaction; D015231:Sexually Transmitted Diseases, Bacterial / Q000453:epidemiology* / Q000382:microbiology*
chemical_list: D001425:Bacterial Outer Membrane Proteins; C078167:OMPA outer membrane proteins