title: [Expression of microRNA-146a in lipopolysaccharide challenged alveolar macrophages in vitro].
abstract: OBJECTIVE
  To investigate the expression of microRNA-146a (miRNA-146a) in NR8383 alveolar macrophages treated with lipopolysaccharides (LPS).
  
  
  METHODS
  NR8383 alveolar macrophages were divided into two groups: LPS treated group and phosphate buffer saline (PBS) control group, and they cultured for 6 hours. The production of tumor necrosis factor-α (TNF-α) in the supernatant of cells was determined with enzyme-linked immunosorbent assay (ELISA), and the expression of miRNA-146a of cells was detected by real-time polymerase chain reaction (PCR).
  
  
  RESULTS
  Compared with PBS control group, the TNF-α content (ng/L) in LPS treated group was significantly increased (650.26±40.53 vs. 6.23±1.76, P<0.01), and miRNA-146a in LPS treated group increased by about (5.33±0.81) folds (P<0.01).
  
  
  CONCLUSIONS
  The expression of miRNA-146a was increased in LPS treated NR8383 cells, and miRNA-146a may be involved in the modulation inflammatory response of the NR8383 alveolar macrophage.
authors.affiliation: Intensive Care Unit, the First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi, China.
authors.forename: Fen
authors.initials: F
authors.lastname: Liu
authors.forename: Zhen-Guo
authors.initials: ZG
authors.lastname: Zeng
authors.forename: Cheng-Zhi
authors.initials: CZ
authors.lastname: Ding
authors.forename: Qiang
authors.initials: Q
authors.lastname: Shao
authors.forename: Yong
authors.initials: Y
authors.lastname: Li
authors.forename: Ke-Jian
authors.initials: KJ
authors.lastname: Qian
mesh_terms: D000818:Animals; D002460:Cell Line; D007249:Inflammation; D008070:Lipopolysaccharides / Q000009:adverse effects; D016676:Macrophages, Alveolar / Q000187:drug effects / Q000378:metabolism*; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D008070:Lipopolysaccharides; C554180:MIRN146 microRNA, rat; D035683:MicroRNAs; D014409:Tumor Necrosis Factor-alpha