Whole body radioprotective activity of an acetone-water extract from the seedpod of Nelumbo nucifera Gaertn. seedpod.
Procyanidins extracted with acetone-water from lotus (Nelumbo nucifera Gaertn.) seedpod (LSPCs) were evaluated for in vivo radioprotective activity against whole body gamma irradiation in Swiss albino mice. Pretreated with LSPCs 200 mg/kg by intragastric (i.g.) for 15 days was found to be the most effective dose in preventing radiation sickness, reducing radiation-induced mortality, increasing mean survival time and elevating radiation median lethal dose (LD(50)) from 8.9 to 10.5 Gy, indicating a dose modifying factor (DMF) of 1.18. Further, administered LSPCs at a dose of 200 mg/kg could effectively maintain spleen index close to normal, stimulate endogenous spleen colony forming units, promote the levels of red blood cells (RBC), white blood cells (WBC), platelets and hemoglobin in peripheral blood, and prevent spleen and skin damage in irradiated mice, reduce the level of radiation-induced micronucleated polychromatic erythrocytes in bone marrow, maintain the polychromatic erythrocytes (PCE) and normochromatic erythrocytes (NCE) ratio (P/N ratio) and significantly decrease bone marrow chromosomal damage. Alternatively, pretreated with LSPCs (200 mg/kg) significantly decreased the lipid peroxidation (LPO) level, and elevated the activities of endogenous antioxidant enzymes in liver after irradiation. Thus LSPCs possess a strong whole body radioprotective activity, and it may be used as a radioprotector.
Duan
Y
Y
School of Food and Biological Engineering, Jiangsu University, Zhenjiang, China. dyq101@ujs.edu.cn
Zhang
H
H
Xie
B
B
Yan
Y
Y
Li
J
J
Xu
F
F
Qin
Y
Y
D000096:Acetone; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001853:Bone Marrow / Q000187:drug effects / Q000648:ultrastructure; D002875:Chromosomes / Q000187:drug effects / Q000648:ultrastructure; D005720:Gamma Rays; D006397:Hematinics / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D051379:Mice; D015162:Micronucleus Tests; D031653:Nelumbo / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology; D011832:Radiation Injuries / Q000473:pathology / Q000517:prevention & control; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D012639:Seeds / Q000737:chemistry; D012997:Solvents; D013154:Spleen / Q000473:pathology / Q000528:radiation effects; D016019:Survival Analysis; D014867:Water; D014916:Whole-Body Irradiation
D000975:Antioxidants; D006397:Hematinics; D010936:Plant Extracts; D011837:Radiation-Protective Agents; D012997:Solvents; D014867:Water; D000096:Acetone