Overexpression of AEG-1 in renal cell carcinoma and its correlation with tumor nuclear grade and progression.
The study was aimed at detecting the expression of a newly found oncogene, astrocyte elevated gene-1 (AEG-1), in renal cell carcinoma (RCC) and its correlation with histopathologic features and the survival of patients. Real-time reverse transcription-PCR and Western blot showed markedly higher expression of AEG-1 in 8 cases of RCC tissue compared with the paired normal tissue from the same patient. The expression level of AEG-1 was also increased in four RCC cell lines in contrast with normal tubular epithelial human kidney cells 2 (HK-2) at both mRNA and protein levels. Furthermore, immunohistochemistry analysis showed highly expressed AEG-1 in 96 of 102 (94.1%) cases of paraffin-embedded archival RCC tissue. Statistical analysis showed a significant correlation of AEG-1 expression with tumor grade (P <0.001), clinical staging (P = 0.003), T classification (P = 0.003) as well as metastasis classification (P=0.032). The means for survival time of low AEG-1 expression group was 76.98m<br />while high AEG-1expression group was 60.94m. Our results suggest that AEG-1 protein is overexpressed in RCC and plays an important role in tumor differentiation and progression. <br />High AEG-1 expression is closely associated with poor prognosis.


BACKGROUND
Astrocyte elevated gene-1, AEG-1, renal cell carcinoma, prognosis.
Chen
W
W
Department of Pathology, Sun Yat-sen University, Guangzhou, China.
Ke
Z
Z
Shi
H
H
Yang
S
S
Wang
L
L
D002292:Carcinoma, Renal Cell / Q000737:chemistry* / Q000473:pathology; D015815:Cell Adhesion Molecules / Q000032:analysis* / Q000235:genetics / Q000502:physiology; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000473:pathology*; D018450:Disease Progression; D006801:Humans; D007150:Immunohistochemistry; D007680:Kidney Neoplasms / Q000737:chemistry* / Q000473:pathology; D008565:Membrane Proteins; D009367:Neoplasm Staging; D016601:RNA-Binding Proteins
D015815:Cell Adhesion Molecules; C483039:MTDH protein, human; D008565:Membrane Proteins; D016601:RNA-Binding Proteins