title: Adding value to a toxic residue from the biodiesel industry: production of two distinct pool of lipases from Penicillium simplicissimum in castor bean waste.
abstract: In countries with a strong agricultural base, such as Brazil, the generation of solid residues is very high. In some cases, these wastes present no utility due to their toxic and allergenic compounds, and so are an environmental concern. The castor bean (Ricinus communis) is a promising candidate for biodiesel production. From the biodiesel production process developed in the Petrobras Research Center using castor bean seeds, a toxic and alkaline waste is produced. The use of agroindustrial wastes in solid-state fermentation (SSF) is a very interesting alternative for obtaining enzymes at low cost. Therefore, in this work, castor bean waste was used, without any treatment, as a culture medium for fungal growth and lipase production. The fungus Penicillium simplicissimum was able to grow and produce an enzyme in this waste. In order to maximize the enzyme production, two sequential designs-Plackett-Burman (variable screening) followed by central composite rotatable design (CCRD)-were carried out, attaining a considerable increase in lipase production, reaching an activity of 155.0 U/g after 96 h of fermentation. The use of experimental design strategy was efficient, leading to an increase of 340% in the lipase production. Zymography showed the presence of different lipases in the crude extract. The partial characterization of such extract showed the occurrence of two lipase pools with distinct characteristics of pH and temperature of action: one group with optimal action at pH 6.5 and 45°C and another one at pH 9.0 and 25°C. These results demonstrate how to add value to a toxic and worthless residue through the production of lipases with distinct characteristics. This pool of enzymes, produced through a low cost methodology, can be applied in different areas of biotechnology.
authors.affiliation: Departamento de Bioquímica, Laboratório de Biotecnologia Microbiana - 549-1 e 2, Instituto de Química, Centro de Tecnologia, Universidade Federal do Rio de Janeiro, Av. Athos da Silveira Ramos, 149, Bloco A, Rio de Janeiro, RJ, CEP 21941-909, Brazil. godoy@iq.ufrj.br
authors.forename: Mateus G
authors.initials: MG
authors.lastname: Godoy
authors.forename: Melissa L E
authors.initials: ML
authors.lastname: Gutarra
authors.forename: Aline M
authors.initials: AM
authors.lastname: Castro
authors.forename: Olga L T
authors.initials: OL
authors.lastname: Machado
authors.forename: Denise M G
authors.initials: DM
authors.lastname: Freire
mesh_terms: D056804:Biofuels / Q000191:economics / Q000382:microbiology*; D001709:Biotechnology; D001938:Brazil; D002367:Ricinus communis / Q000378:metabolism*; D002617:Chemical Industry; D003470:Culture Media / Q000737:chemistry; D005285:Fermentation; D015386:Hazardous Substances / Q000378:metabolism* / Q000633:toxicity; D006863:Hydrogen-Ion Concentration; D008049:Lipase / Q000378:metabolism*; D010407:Penicillium / Q000201:enzymology* / Q000254:growth & development; D012037:Refuse Disposal / Q000379:methods; D013696:Temperature; D014866:Waste Products
chemical_list: D056804:Biofuels; D003470:Culture Media; D015386:Hazardous Substances; D014866:Waste Products; D008049:Lipase