title: Endothelial integrity of radial artery grafts harvested by minimally invasive surgery--immunohistochemical studies of CD31 and endothelial nitric oxide synthase expressions: a randomized controlled trial.
abstract: OBJECTIVE
  To compare the endothelial integrity of radial artery grafts harvested by minimally invasive surgery and arteries harvested conventionally for coronary artery bypass surgery (CABG) in 200 participants, who were assigned to interventions by using random allocation.
  
  
  METHODS
  An immunohistochemical procedure with monoclonal antibodies was employed to estimate CD31 antigen and endothelial nitric oxide synthase (eNOS) expressions - markers defining endothelial integrity.
  
  
  RESULTS
  The CD31 immunostaining revealed that the endothelial cell integrity of the minimally invasive harvested arteries was preserved in 76.1±7.4% of the circumference of luminal endothelium, which was similar to results obtained in conventionally harvested grafts (77.2±9.8%; not significant). On the other hand, eNOS immunostaining indicated that the endothelial integrity of the minimally invasive harvested grafts was preserved in 75.4±10.5% while in conventionally harvested grafts it was reduced to 42.4±14.5% of the total luminal endothelium circumference (P<0.05).
  
  
  CONCLUSIONS
  The endothelial integrity of radial artery grafts harvested by minimally invasive surgery is better preserved than in the grafts obtained by the conventional manner. This could play an important role in improving graft patency and might represent a preliminary condition of stable functioning in coronary arterial bypasses.
authors.affiliation: Regional Specialized Hospital in Wroclaw, Research and Development Centre, Wroclaw, Poland. mnowicki@ump.edu.pl
authors.forename: Michal
authors.initials: M
authors.lastname: Nowicki
authors.forename: Marcin
authors.initials: M
authors.lastname: Misterski
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Malinska
authors.forename: Bartlomiej
authors.initials: B
authors.lastname: Perek
authors.forename: Danuta
authors.initials: D
authors.lastname: Ostalska-Nowicka
authors.forename: Marek
authors.initials: M
authors.lastname: Jemielity
authors.forename: Wojciech
authors.initials: W
authors.lastname: Witkiewicz
authors.forename: Maciej
authors.initials: M
authors.lastname: Zabel
mesh_terms: D000368:Aged; D004730:Endothelium, Vascular / Q000378:metabolism / Q000637:transplantation*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D019060:Minimally Invasive Surgical Procedures; D009131:Muscle, Smooth, Vascular / Q000378:metabolism; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism*; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism*; D011446:Prospective Studies; D017534:Radial Artery / Q000378:metabolism / Q000637:transplantation*; D012501:Saphenous Vein / Q000378:metabolism / Q000637:transplantation; D020858:Tissue and Organ Harvesting / Q000379:methods*; D016896:Treatment Outcome
chemical_list: D019408:Platelet Endothelial Cell Adhesion Molecule-1; D052250:Nitric Oxide Synthase Type III