Detection of swine-origin influenza A (H1N1) viruses using a localized surface plasmon coupled fluorescence fiber-optic biosensor.
Swine-origin influenza A (H1N1) virus (S-OIV) was identified as a new reassortant strain of influenza A virus in April 2009 and led to an influenza pandemic. Accurate and timely diagnoses are crucial for the control of influenza disease. We developed a localized surface plasmon coupled fluorescence fiber-optic biosensor (LSPCF-FOB) which combines a sandwich immunoassay with the LSP technique using antibodies against the hemagglutinin (HA) proteins of S-OIVs. The detection limit of the LSPCF-FOB for recombinant S-OIV H1 protein detection was estimated at 13.9 pg/mL, which is 10(3)-fold better than that of conventional capture ELISA when using the same capture antibodies. For clinical S-OIV isolates measurement, meanwhile, the detection limit of the LSPCF-FOB platform was calculated to be 8.25 × 10(4)copies/mL, compared with 2.06 × 10(6)copies/mL using conventional capture ELISA. Furthermore, in comparison with the influenza A/B rapid test, the detection limit of the LSPCF-FOB for S-OIV was almost 50-fold in PBS solution and 25-fold lower in mimic solution, which used nasal mucosa from healthy donors as the diluent. The findings of this study therefore indicate that the high detection sensitivity and specificity of the LSPCF-FOB make it a potentially effective diagnostic tool for clinical S-OIV infection and this technique has the potential to be applied to the development of other clinical microbe detection platforms.
Chang
Ying-Feng
YF
Institute of Biophotonics, National Yang-Ming University, Taipei 112, Taiwan.
Wang
Sheng-Fan
SF
Huang
Jason C
JC
Su
Li-Chen
LC
Yao
Ling
L
Li
Ying-Chang
YC
Wu
Suh-Chin
SC
Chen
Yi-Ming A
YM
Hsieh
Jo-Ping
JP
Chou
Chien
C
D000818:Animals; D000914:Antibodies, Viral; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D005453:Fluorescence; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000032:analysis / Q000235:genetics / Q000276:immunology; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D007251:Influenza, Human / Q000175:diagnosis / Q000453:epidemiology / Q000821:virology; D055100:Optical Fibers; D058873:Pandemics; D016865:Reassortant Viruses / Q000235:genetics / Q000302:isolation & purification*; D011994:Recombinant Proteins / Q000032:analysis / Q000235:genetics / Q000276:immunology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D020349:Surface Plasmon Resonance / Q000295:instrumentation* / Q000379:methods / Q000706:statistics & numerical data; D013552:Swine / Q000821:virology*
D000914:Antibodies, Viral; D017931:DNA Primers; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D011994:Recombinant Proteins