title: Using the Velvet de novo assembler for short-read sequencing technologies.
abstract: The Velvet de novo assembler was designed to build contigs and eventually scaffolds from short-read sequencing data. This protocol describes how to use Velvet, interpret its output, and tune its parameters for optimal results. It also covers practical issues such as configuration, using the VelvetOptimiser routine, and processing colorspace data.
authors.affiliation: Center for Biomolecular Science and Engineering, Santa Cruz, California, USA.
authors.forename: Daniel R
authors.initials: DR
authors.lastname: Zerbino
mesh_terms: D001483:Base Sequence; D017422:Sequence Analysis, DNA / Q000379:methods*; D012984:Software