title: Influence of the albumin concentration and temperature on the lysis of human erythrocytes by sodium dodecyl sulfate.
abstract: The stability of human erythrocytes to sodium dodecyl sulfate (SDS) was assessed spectrophotometrically in the presence of different concentrations of bovine serum albumin (BSA) and at different temperatures (27-45 °C). The absorbance at 540 nm (A₅₄₀) was correlated with the SDS concentration by sigmoidal regression based on the Boltzmann equation. Erythrocyte stability was characterized on the basis of the SDS concentration that induces hemolysis in 50% of the cells (D₅₀). Progressive increases in the albumin concentration led to increases in the D₅₀ value. The protective effect of BSA against SDS-induced hemolysis was attributed to the binding of the surfactant to the hydrophobic binding sites of this protein. The D₅₀ values decreased sigmoidally with an increase in the temperature. This trend, which could not be explained by changes in the spectral properties of hemoglobin, maybe due to heterogeneity in the erythrocyte population.
authors.affiliation: Institute of Genetics and Biochemistry, Federal University of Uberlândia, Uberlândia, MG 38400-902, Brazil.
authors.forename: L C
authors.initials: LC
authors.lastname: Fonseca
authors.forename: L R
authors.initials: LR
authors.lastname: Arvelos
authors.forename: R C M
authors.initials: RC
authors.lastname: Netto
authors.forename: A B
authors.initials: AB
authors.lastname: Lins
authors.forename: M S
authors.initials: MS
authors.lastname: Garrote-Filho
authors.forename: N
authors.initials: N
authors.lastname: Penha-Silva
mesh_terms: D000818:Animals; D002417:Cattle; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism*; D006461:Hemolysis; D006801:Humans; D012044:Regression Analysis; D012710:Serum Albumin, Bovine / Q000494:pharmacology*; D012967:Sodium Dodecyl Sulfate / Q000378:metabolism / Q000633:toxicity*; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature
chemical_list: D012710:Serum Albumin, Bovine; D012967:Sodium Dodecyl Sulfate