Anti-fibrillin-1 autoantibodies in normal pregnancy and recurrent pregnancy loss.
OBJECTIVE
The aim of this study was to investigate anti-fibrillin-1 autoantibody in patients with a history of recurrent pregnancy loss (RPL) and during normal pregnancy.


METHODS
Anti-fibrillin-1 IgG and IgM antibodies were measured by a home made ELISA in serum samples of 48 medically and obstetrically normal pregnant women, classified to three trimester groups, 15 female patients with RPL and 26 healthy non-pregnant women classified to two control subgroups: (a) women who had already had at least one previous successful pregnancy and (b) women who had never been pregnant. Differences in anti-fibrillin-1 autoantibodies between the groups were analyzed for statistical significance (P<0.05) with one-way analysis of variance (ANOVA) and multiple comparison test - Post Hoc test, Least Significant Difference method.


RESULTS
Anti-fibrillin-1 IgM autoantibodies were significantly decreased in the second and third trimester pregnant women compared to the nulligravida controls. RPL patients had significantly increased anti-fibrillin-1 IgM antibody compared to control group (a).


CONCLUSIONS
Fibrillin-1 degradation seems to be decreased during the second and third trimester of normal pregnancy. Increased anti-fibrillin-1 IgM antibodies in RPL patients may be a secondary phenomenon of increased fibrillin-1 degradation and contribute to the pathogenesis of pregnancy losses.
Atanasova
Milena Atanasova
MA
Department of Biology, Medical University of Pleven, 1, Kliment Ohridski Str., Pleven, 5800, Bulgaria.
Konova
Emiliana Ilieva
EI
Aleksovska
Tania Ace
TA
Todorova
Katia Nikolova
KN
Georgieva
Miglena Nikolaeva
MN
Lukanov
Tzvetan Hristoforov
TH
D000026:Abortion, Habitual / Q000097:blood* / Q000276:immunology; D000293:Adolescent; D000328:Adult; D001323:Autoantibodies / Q000276:immunology / Q000378:metabolism*; D005260:Female; D000071838:Fibrillin-1; D000071837:Fibrillins; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood* / Q000276:immunology; D007075:Immunoglobulin M / Q000097:blood* / Q000276:immunology; D008840:Microfilament Proteins / Q000097:blood* / Q000276:immunology; D011247:Pregnancy / Q000097:blood* / Q000276:immunology; D011262:Pregnancy Trimester, Second / Q000097:blood / Q000276:immunology; D011263:Pregnancy Trimester, Third / Q000097:blood / Q000276:immunology; D054392:Adipokines
D001323:Autoantibodies; D000071838:Fibrillin-1; D000071837:Fibrillins; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D008840:Microfilament Proteins; C000605722:FBN1 protein, human; D054392:Adipokines