Label-free detection of lectins on carbohydrate-modified boron-doped diamond surfaces.
This paper describes the label-free detection of carbohydrate-lectin interactions. The sensor consists of a boron-doped diamond (BDD) electrode terminated with alkynyl surface groups, which have been functionalized via the CuACC (copper(I)-catalyzed azide-alkyne cycloaddition) "click" reaction with carbohydrate analogues bearing an azido-terminating arm. In this work, electrochemical impedance spectroscopy (EIS) was used as an effective technique to probe the specific interactions of the surface-bound carbohydrates with their complementary lectin partners, and the response was found to be dependent on the relative density of sugar units immobilized on the BDD surface. A BDD interface with 60% surface-bound mannose showed a detection limit of ∼5 ± 0.5 nM for Lens culinaris lectin, with an affinity constant of K(A) = (2.63 ± 0.5) × 10(6) M(-1).
Szunerits
Sabine
S
Institut de Recherche Interdisciplinaire (IRI, USR 3078), Université Lille Nord de France, Parc de la Haute Borne, 50 Avenue de Halley, BP 70478, 59658 Villeneuve d'Ascq, France. sabine.szunerits@iri.univ-lille1.fr
Niedziołka-Jönsson
Joanna
J
Boukherroub
Rabah
R
Woisel
Patrice
P
Baumann
Jean-Sébastien
JS
Siriwardena
Aloysius
A
D001895:Boron / Q000737:chemistry*; D002384:Catalysis; D003300:Copper / Q000737:chemistry; D018130:Diamond / Q000737:chemistry*; D058266:Dielectric Spectroscopy / Q000379:methods*; D055664:Electrochemical Techniques / Q000379:methods*; D004566:Electrodes; D005292:Ferricyanides / Q000737:chemistry; D037102:Lectins / Q000032:analysis*; D027806:Lens Plant / Q000378:metabolism; D008358:Mannose / Q000737:chemistry*; D013499:Surface Properties
D005292:Ferricyanides; D037102:Lectins; C007931:hexacyanoferrate III; D018130:Diamond; D003300:Copper; D001895:Boron; D008358:Mannose