title: Differential effect of CD4+Foxp3+ T-regulatory cells on the B and T helper cell responses to influenza virus vaccination.
abstract: The T-regulatory (T-reg) cells restrict the T-cell functions in various viral infections including influenza infection. However little is known about the effect of T-regs in influenza vaccination. Herein, we found that immunization of BALB/c mice with a prototype of UV-inactivated influenza PR8/A/34 virus vaccine expanded the CD4(+)Foxp3(+) T-reg pool and fostered the development of virus-specific CD4(+)Foxp3(+) T-reg cells. Increasing the size of Foxp3(+) T-reg pool did not alter the primary PR8-specific B-cell response, but it did suppress the primary and memory PR8-specific T helper responses induced by vaccination. In contrast, the vaccination-induced T helper cell response was augmented in the absence of CD4(+)Foxp3(+) T-reg cells. Since CD4 T helper cells contribute to anti-influenza protection, therapeutic "quenching" of T-reg function prior to vaccination may enhance the efficacy of influenza vaccination.
authors.affiliation: Department of Medicine, Division of Immunology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA.
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Surls
authors.forename: Cristina
authors.initials: C
authors.lastname: Nazarov-Stoica
authors.forename: Margaret
authors.initials: M
authors.lastname: Kehl
authors.forename: Sofia
authors.initials: S
authors.lastname: Casares
authors.forename: Teodor-D
authors.initials: TD
authors.lastname: Brumeanu
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D051858:Forkhead Transcription Factors / Q000276:immunology; D007111:Immunity, Cellular; D007156:Immunologic Memory; D009980:Influenza A virus / Q000276:immunology; D007252:Influenza Vaccines / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D018345:Mice, Knockout; D009976:Orthomyxoviridae Infections / Q000276:immunology* / Q000517:prevention & control; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D015164:Vaccines, Inactivated / Q000276:immunology
chemical_list: D051858:Forkhead Transcription Factors; C498833:Foxp3 protein, mouse; D007252:Influenza Vaccines; D015164:Vaccines, Inactivated