A D-galactose-binding lectin purified from coronate moon turban, Turbo (Lunella) coreensis, with a unique amino acid sequence and the ability to recognize lacto-series glycosphingolipids.
A divalent, cation-independent d-galactose-binding lectin was purified from coronate moon turban Turbo (Lunella) coreensis. This lectin recognizes d-galactose and is a 38-kDa dimeric protein consisting disulphide-bonded 22-kDa polypeptides under non-reducing and reducing conditions of sodium dodecyl sulphate-polyacrylamide gel electrophoresis, respectively. Haemagglutination activity was inhibited by D-galactose, N-acetyl D-galactosamine, melibiose, lactose, porcine stomach mucin, asialofetuin and bovine submaxillary mucin. The lectin has tolerance for pH 5-11 and temperature until 50°C for 1h. The lectin strongly aggregated Gram-negative bacteria, such as Vibrio parahaemolyticus and Salmonella O7, but weakly Gram-positive strain as Staphylococcus aureus and Bacillus subtilis. The glycan-binding profile of this lectin was evaluated using frontal affinity chromatography technology and the lectin appeared to recognize oligosaccharides such as lacto-series glycosphingolipids contained in blood type A and H substances in addition to complex-type N-linked glycoproteins. Partial primary structures of 139 amino acid residues of this lectin were determined from N-terminus polypeptides and 8 peptides derived by cleavage with lysyl-endopeptidase. The primary structure was slightly similar to other known sequences of lectin; however, a repeating motif has been included.
Fujii
Yuki
Y
Laboratory of Glycobiology and Marine Biochemistry, Department of Genome System Science, Graduate School of NanoBioscience, Yokohama City University, 22-2 Seto, Kanazawa-ku, Yokohama, 236-0027, Japan.
Kawsar
Sarkar M A
SM
Matsumoto
Ryo
R
Yasumitsu
Hidetaro
H
Ishizaki
Naoto
N
Dogasaki
Chikaku
C
Hosono
Masahiro
M
Nitta
Kazuo
K
Hamako
Jiharu
J
Taei
Matsui
M
Ozeki
Yasuhiro
Y
D000595:Amino Acid Sequence; D000818:Animals; D002846:Chromatography, Affinity; D037161:Galectins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D049851:Gastropoda / Q000737:chemistry*; D006028:Glycosphingolipids / Q000737:chemistry* / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D013379:Substrate Specificity; D013696:Temperature
D037161:Galectins; D006028:Glycosphingolipids