Spectroscopic investigation on assisted sonocatalytic damage of bovine serum albumin (BSA) by metronidazole (MTZ) under ultrasonic irradiation combined with nano-sized ZnO.
The previous work proved that the bovine serum albumin (BSA) could be damaged under the combined action of ultrasonic irradiation and ZnO. In this work, the assisted sonocatalytic damage of BSA using metronidazole (MTZ) as a sensitizer was further investigated by means of UV-vis and fluorescence spectra. The results indicated that the adding of MTZ could obviously promote the sonocatalytic damage of BSA under ultrasonic irradiation in the presence of nano-sized ZnO powder. Furthermore, it was found that the damage degree of BSA was aggravated by some influencing factors except ionic kind and strength. In addition, the damage site of BSA was also studied with synchronous fluorescence technology. It was found that the damage site was mainly at tryptophan (Trp) residue.
Gao
Jingqun
J
Department of Chemistry, Liaoning University, Shenyang 110036, PR China.
Liu
Bin
B
Wang
Jun
J
Jin
Xudong
X
Jiang
Renzheng
R
Liu
Lijun
L
Wang
Baoxin
B
Xu
Yongnan
Y
D000818:Animals; D002417:Cattle; D008795:Metronidazole / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry; D011838:Radiation-Sensitizing Agents; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000528:radiation effects*; D013057:Spectrum Analysis; D014364:Tryptophan; D014465:Ultrasonics; D015034:Zinc Oxide / Q000737:chemistry*
D011838:Radiation-Sensitizing Agents; D008795:Metronidazole; D012710:Serum Albumin, Bovine; D014364:Tryptophan; D015034:Zinc Oxide