title: [The expression of CD95 and activated antigens in peripheral blood lymphocyte subsets in patients with hand foot and mouth disease (HFMD)].
abstract: OBJECTIVE
  To investigate the expression of CD95 and special marker for activation of peripheral blood lymphocytes in patients with hand foot and mouth disease (HFMD) and its significance.
  
  
  METHODS
  Immunofluorescent two-color flow cytometry was used to study the expression of CD95 and HLA-DR on lymphocytes in 58 patients with HFMD and 34 normal controls.
  
  
  RESULTS
  Expression of CD3+ T cells was significantly lower in patients (63.82 +/- 7.74)% than that in controls (P < 0.001), meanwhile the expression of CD4+ T cells was (34.29 +/- 7.33)%, significantly lower than that of the controls (P < 0.005). The percentage of lymphocytes expressing HLA-DR in patients was (23.77 +/- 5.78)%, significantly higher than that of the controls (P < 0.005). Significant difference was observed in the expression of HLA- DR on CD8+ T cells in patients (1.34 +/- 1.12)% as compared with controls (P < 0.005). No significant difference in the expression of CD95 on lymphocytes was observed between patients and the controls (P > 0.05).
  
  
  CONCLUSIONS
  The findings support that cellular immunodeficiency exists in patients and that lymphocytes were abnormally activated in the patients. The activation of peripheral blood T lymphocytes in patients mainly involves CD8 subset and it may play an important role in the immune response to antiviral infection.
authors.affiliation: Open laboratory of Hangzhou sixth people's Hospital, Hangzhou 310014, China. mlzhhz@163.com
authors.forename: Ming-Li
authors.initials: ML
authors.lastname: Zhu
authors.forename: Song-Ping
authors.initials: SP
authors.lastname: Zhang
authors.forename: He-Qian
authors.initials: HQ
authors.lastname: Cao
authors.forename: Wei-Feng
authors.initials: WF
authors.lastname: Liang
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Yu
authors.forename: Guo-Qiang
authors.initials: GQ
authors.lastname: Lou
mesh_terms: D000941:Antigens / Q000235:genetics* / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D016022:Case-Control Studies; D002478:Cells, Cultured; D002675:Child, Preschool; D006232:Hand, Foot and Mouth Disease / Q000235:genetics* / Q000276:immunology*; D006801:Humans; D007223:Infant; D018655:Lymphocyte Count; D008297:Male; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D019014:fas Receptor / Q000235:genetics* / Q000276:immunology
chemical_list: D000941:Antigens; D019014:fas Receptor