title: Expression of TLR4-MyD88 and NF-κB in the iris during endotoxin-induced uveitis.
abstract: OBJECTIVE
  To observe the expression of Toll-like receptor-4 (TLR4), myeloid differentiation factor 88 (MyD88), and nuclear factor kappa B p65 (NF-κB p65) in iris tissue during endotoxin-induced uveitis (EIU) and evaluate the significance of these factors in uveitis.
  
  
  METHODS
  Wistar rats were randomly divided into 5 groups (0  h, 12  h, 24  h, 48  h, and 72  h, n = 10/group). Animal model of acute anterior uveitis was established by a hind footpad injection of 200  μg Cholera vibrio LPS. Expression of TLR4, MyD88, and NF-κB p65 in iris ciliary body tissue was detected through immunohistochemical staining.
  
  
  RESULTS
  Expression of TLR4 was not detected in normal iris-ciliary body complex, TLR4 positive cells with round morphology appeared in the iris stroma 12 hours after injection, significantly increased (P < .001) 48 hours after injection, and decreased gradually 72 hours after injection. Expression of MyD88 and NF-κB p65 is consistent with the change of the TLR4.
  
  
  CONCLUSIONS
  The increased expression of TLR4 and its downstream signal transduction moleculesMyD88, NF-κB p65 indicate the potential role of pathway in the pathogenesis of acute anterior uveitis (AAU).
authors.affiliation: Departmnet of Ophthalmology, Beijing Chaoyang Hospital, Capital Medical University, No. 8 Baijiazhuang Road, Chaoyang District, Beijing 100020, China.
authors.forename: Shang
authors.initials: S
authors.lastname: Li
authors.forename: Hong
authors.initials: H
authors.lastname: Lu
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Hu
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.forename: Yingzhi
authors.initials: Y
authors.lastname: Xu
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
mesh_terms: D000818:Animals; D004731:Endotoxins / Q000633:toxicity*; D007150:Immunohistochemistry; D007498:Iris / Q000378:metabolism*; D008297:Male; D053594:Myeloid Differentiation Factor 88 / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D051197:Toll-Like Receptor 4 / Q000378:metabolism*; D014605:Uveitis / Q000139:chemically induced* / Q000378:metabolism*
chemical_list: D004731:Endotoxins; D053594:Myeloid Differentiation Factor 88; D016328:NF-kappa B; D051197:Toll-Like Receptor 4