Diabetes mellitus- and cooling-induced bladder contraction: an in vitro study.
The effects of diabetes mellitus during cooling on ACh- and KCl-induced responses were investigated in rat urinary bladder. Diabetes was induced in the rats by 50 mg/kg streptozotocin via an intraperitoneal injection. Rats' body and bladder weights were measured. The isometric tension to ACh (10(-9) - 3 × 10(-4) M) and KCl (5-100 mM) in strips of urinary detrusor muscle of diabetic and non-diabetic rats, in organ baths at 37 and 28ºC were recorded. The body weights were significantly decreased and the bladder weights increased in STZ-induced diabetic group compared to the non-diabetic group. ACh and KCl caused concentration-dependent contractions of urinary bladders from non-diabetic and STZ-induced diabetic rats. During cooling, the sensitivity and the maximal response were significantly higher than those during 37ºC, both in non-diabetic and diabetic preparations. Cooling of detrusor muscle preparations induces a graded contraction inversely proportional to the temperature in diabetic rats. It may be assumed that the cooling response involves the same mechanisms in the diabetic and non-diabetic animals.
Atalik
Kismet Esra
KE
Department of Pharmacology, Faculty of Meram Medicine, University of Selçuk, Turkey. esraatalik@hotmail.com
Okudan
Nilsel
N
Gokbel
Hakki
H
Kalkan
Serpil
S
Cuce
Gokhan
G
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D002217:Carbachol / Q000494:pharmacology; D003080:Cold Temperature; D003921:Diabetes Mellitus, Experimental / Q000503:physiopathology*; D005260:Female; D066298:In Vitro Techniques; D007274:Injections, Intraperitoneal; D009119:Muscle Contraction; D009130:Muscle, Smooth / Q000473:pathology; D011189:Potassium Chloride / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013311:Streptozocin / Q000737:chemistry; D001743:Urinary Bladder / Q000473:pathology*; D001745:Urinary Bladder Diseases / Q000503:physiopathology*
D013311:Streptozocin; D011189:Potassium Chloride; D002217:Carbachol; D000109:Acetylcholine