Distinct immune responses of recombinant plasmid DNA replicon vaccines expressing two types of antigens with or without signal sequences.
Here, DNA replicon vaccines encoding the Hc domain of botulinum neurotoxin serotype A (AHc) or the receptor binding domain of anthrax protective antigen (PA4) with or without signal sequences were evaluated in mice. Strong antibody and protective responses were elicited only from AHc DNA vaccines with an Ig κ signal sequence or tissue plasminogen activator signal sequence. Meanwhile, there were no differences in total antibody responses or isotypes, lymphocyte proliferative responses, cytokine profiles and protective immune responses with the PA4 DNA vaccines with or without a signal sequence. Therefore, use of targeting sequences in designing DNA replicon vaccines depends on the specific antigen.
Yu
Yun-Zhou
YZ
Beijing Institute of Biotechnology, 20 Dong Dajie Street, Fengtai District, Beijing 100071, China. yunzhouyu@163.com
Li
Na
N
Wang
Wen-Bin
WB
Wang
Shuang
S
Ma
Yao
Y
Yu
Wei-Yuan
WY
Sun
Zhi-Wei
ZW
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000276:immunology*; D001427:Bacterial Toxins / Q000276:immunology*; D001428:Bacterial Vaccines / Q000235:genetics / Q000276:immunology*; D019274:Botulinum Toxins, Type A / Q000276:immunology*; D049109:Cell Proliferation; D016207:Cytokines / Q000097:blood; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids; D021382:Protein Sorting Signals; D012093:Replicon; D013047:Specific Pathogen-Free Organisms; D013154:Spleen / Q000166:cytology / Q000276:immunology; D019444:Vaccines, DNA / Q000235:genetics / Q000276:immunology*
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001427:Bacterial Toxins; D001428:Bacterial Vaccines; D016207:Cytokines; D021382:Protein Sorting Signals; D019444:Vaccines, DNA; C030325:anthrax toxin; D019274:Botulinum Toxins, Type A