title: [Hemocompatibility evaluation in vitro of small-caliber expanded polytetrafluoroethylene vessel with silk fibroin coating sulfonated by low temperature plasma].
abstract: OBJECTIVE
  To evaluate the hemocompatibility of a small-caliber expanded polytetrafluoroethylene vessel with silk fibroin coating sulfonated by low temperature plasma treatment.
  
  
  METHODS
  The composite blood vessel was prepared by first coating the small-caliber expanded polytetrafluoroethylene vessel with silk fibroin followed by sulfonation by low temperature plasma treatment. After hemolysis test in vitro, dynamic coagulation time test, blood platelet adhesion test, and recalcification time test were performed to evaluate the hemocompatibility of the composite blood vessel.
  
  
  RESULTS
  Scanning electronic microscopy revealed obvious platelets adhesion on the conventional artificial (control) vessel, which seldom occurred on the composite vessel. The curve of absorbance-clotting time of the composite vessel declined more slowly than that of the control vessel. The recalcification time of the composite blood vessel averaged 603 s, significantly longer than that of the control vessel (480 s, P = 0.000).
  
  
  CONCLUSIONS
  The composite blood vessel has good antithrombotic activity and hemocompatibility as a promising vascular prosthesis.
authors.affiliation: Department of Cardiothoracic Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China. lsb19821023.student@sina.com
authors.forename: Shao-bin
authors.initials: SB
authors.lastname: Li
authors.forename: Yu-sheng
authors.initials: YS
authors.lastname: Yan
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.forename: Kun-tang
authors.initials: KT
authors.lastname: Chen
authors.forename: Jian
authors.initials: J
authors.lastname: Tong
authors.forename: Qun-qing
authors.initials: QQ
authors.lastname: Chen
authors.forename: Fu-wei
authors.initials: FW
authors.lastname: Zhang
authors.forename: Fei
authors.initials: F
authors.lastname: Xiao
mesh_terms: D001807:Blood Vessel Prosthesis; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D003080:Cold Temperature; D005349:Fibroins / Q000737:chemistry*; D006801:Humans; D008422:Materials Testing; D058626:Plasma Gases; D011138:Polytetrafluoroethylene / Q000737:chemistry*; D013451:Sulfonic Acids / Q000737:chemistry
chemical_list: D020099:Coated Materials, Biocompatible; D058626:Plasma Gases; D013451:Sulfonic Acids; C482288:fibroin, silkworm; D011138:Polytetrafluoroethylene; D005349:Fibroins