title: Novel dialkoxytriazine-type glycosyl donors for cellulase-catalysed lactosylation.
abstract: Novel glycosidic compounds, 4,6-dialkoxy-1,3,5-triazin-2-yl β-lactosides (DAT-β-Lac), have been prepared directly in water from lactose. The reaction was carried out on a laboratory scale without protecting the hydroxy groups of lactose. The resulting triazine derivatives were found to be recognized by endo-β1,4-glucanase III from Trichoderma reesei (EGIII). The EGIII-catalysed transglycosylation of 4,6-dimethoxy-1,3,5-triazine derivative (DMT-β-Lac) with various glycosyl acceptors has successfully been demonstrated, affording the corresponding lactosylated products.
authors.affiliation: Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University, 6-6-11-514 Aoba, Sendai, Miyagi 980-8579, Japan.
authors.forename: Tomonari
authors.initials: T
authors.lastname: Tanaka
authors.forename: Masato
authors.initials: M
authors.lastname: Noguchi
authors.forename: Kazuhito
authors.initials: K
authors.lastname: Watanabe
authors.forename: Takuya
authors.initials: T
authors.lastname: Misawa
authors.forename: Masaki
authors.initials: M
authors.lastname: Ishihara
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kobayashi
authors.forename: Shin-Ichiro
authors.initials: S
authors.lastname: Shoda
mesh_terms: D055162:Biocatalysis; D002236:Carbohydrate Conformation; D002480:Cellulase / Q000378:metabolism*; D006027:Glycosides / Q000737:chemistry / Q000378:metabolism*; D006031:Glycosylation; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007785:Lactose / Q000737:chemistry / Q000378:metabolism*; D014227:Triazines / Q000737:chemistry / Q000378:metabolism*; D014242:Trichoderma / Q000201:enzymology*
chemical_list: D006027:Glycosides; D014227:Triazines; D002480:Cellulase; D007785:Lactose