title: Tetrazine-trans-cyclooctene ligation for the rapid construction of 18F labeled probes.
abstract: A radiolabeling method for bioconjugation based on the Diels-Alder reaction between 3,6-diaryl-s-tetrazines and an (18)F-labeled trans-cyclooctene is described. The reaction proceeds with exceptionally fast rates, making it an effective conjugation method within seconds at low micromolar concentrations.
authors.affiliation: Molecular Imaging Center, Department of Radiology, University of Southern California, Los Angeles 90033, USA. ziboli@usc.edu
authors.forename: Zibo
authors.initials: Z
authors.lastname: Li
authors.forename: Hancheng
authors.initials: H
authors.lastname: Cai
authors.forename: Matthew
authors.initials: M
authors.lastname: Hassink
authors.forename: Melissa L
authors.initials: ML
authors.lastname: Blackman
authors.forename: Richard C D
authors.initials: RC
authors.lastname: Brown
authors.forename: Peter S
authors.initials: PS
authors.lastname: Conti
authors.forename: Joseph M
authors.initials: JM
authors.lastname: Fox
mesh_terms: D002851:Chromatography, High Pressure Liquid; D034242:Cyclooctanes / Q000737:chemistry*; D005462:Fluorine Radioisotopes / Q000737:chemistry*; D011874:Radiometry
chemical_list: D034242:Cyclooctanes; D005462:Fluorine Radioisotopes