title: Analysis and construction of pathogenicity island regulatory pathways in Salmonella enterica serovar Typhi.
abstract: Signal transduction through protein-protein interactions and protein modifications are the main mechanisms controlling many biological processes. Here we described the implementation of MedScan information extraction technology and Pathway Studio software (Ariadne Genomics Inc.) to create a Salmonella specific molecular interaction database. Using the database, we have constructed several signal transduction pathways in Salmonella enterica serovar Typhi which causes Typhoid Fever, a major health threat especially in developing countries. S. Typhi has several pathogenicity islands that control rapid switching between different phenotypes including adhesion and colonization, invasion, intracellular survival, proliferation, and biofilm formation in response to environmental changes. Understanding of the detailed mechanism for S. Typhi survival in host cells is necessary for development of efficient detection and treatment of this pathogen. The constructed pathways were validated using publically available gene expression microarray data for Salmonella.
authors.affiliation: Centre for Chemical Biology, Universiti Sains Malaysia, 1st Floor, Block B, No 10, Persiaran Bukit Jambul, 11900 Bayan Lepas, Pulau Pinang, Malaysia. moc.liamg@nullbccnaeyus
authors.forename: Su Yean
authors.initials: SY
authors.lastname: Ong
authors.forename: Fui Ling
authors.initials: FL
authors.lastname: Ng
authors.forename: Siti Suriawati
authors.initials: SS
authors.lastname: Badai
authors.forename: Anton
authors.initials: A
authors.lastname: Yuryev
authors.forename: Maqsudul
authors.initials: M
authors.lastname: Alam
mesh_terms: D019295:Computational Biology / Q000379:methods*; D030541:Databases, Genetic; D003906:Developing Countries; D024881:Drug Resistance, Bacterial; D018432:Drug Resistance, Multiple; D044404:Genomic Islands; D006801:Humans; D008264:Macrophages / Q000378:metabolism; D008957:Models, Genetic; D020411:Oligonucleotide Array Sequence Analysis; D010641:Phenotype; D012485:Salmonella typhi / Q000235:genetics*; D015398:Signal Transduction; D014435:Typhoid Fever / Q000235:genetics