title: Antioxidant and hepatoprotective effects of Murdannia bracteata methanol extract.
abstract: Murdannia bracteata (C. B. Clarke) is a local plant that is widely used in Malaysia as a traditional remedy for various diseases of the kidney and liver, including inflammation and cancer. In the present study, we investigated the antioxidant and hepatoprotective activities of M. bracteata methanol extract (MB). 2,2'-diphenyl-1-picrylhydrazyl radical scavenging activity, lipid peroxidation inhibition and trolox equivalent antioxidant capacity of MB were determined. The hepatoprotective activity of MB was studied using a CCl(4)-induced liver toxicity model in rats. The hepatoprotective effect was assessed by monitoring the plasma malondialdehyde level and serum alanine transaminase and aspartate transaminase activities. Histopathological changes of hepatic tissue were also investigated. The results indicated that MB possessed potential antioxidant, lipid peroxidation inhibition and free radical scavenging activities. Pretreatment of rats with MB (500 mg/kg and 1000 mg/kg per os) before induction of CCl(4)-induced hepatotoxicity showed a dose-dependent reduction in the necrotic changes in hepatic tissue. The increases in plasma malondialdehyde level, serum alanine transaminase and aspartate transaminase activities were also significantly inhibited by MB. The total phenolic content of MB determined using Folin-Ciocalteu assay was found to be 10%. The results of the present study indicated that the hepatoprotective effect of MB is most likely due to its antioxidant and free radical scavenging properties.
authors.affiliation: School of Pharmaceutical Sciences, Universiti Sains Malaysia, Pulau Pinang, Malaysia. yammunfei@yahoo.com
authors.forename: Mun Fei
authors.initials: MF
authors.lastname: Yam
authors.forename: Lee Fung
authors.initials: LF
authors.lastname: Ang
authors.forename: Chung Pin
authors.initials: CP
authors.lastname: Lim
authors.forename: Omar Ziad
authors.initials: OZ
authors.lastname: Ameer
authors.forename: Ibrahim Muhammad
authors.initials: IM
authors.lastname: Salman
authors.forename: Mariam
authors.initials: M
authors.lastname: Ahmad
authors.forename: Mahfoudh Al-Musli
authors.initials: MA
authors.lastname: Mohammed
authors.forename: Mohd Zaini
authors.initials: MZ
authors.lastname: Asmawi
authors.forename: Muthanna Fawzy
authors.initials: MF
authors.lastname: Abdulkarim
authors.forename: Ghassan Zuhair
authors.initials: GZ
authors.lastname: Abdullah
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D034821:Commelinaceae / Q000737:chemistry*; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D000432:Methanol / Q000737:chemistry; D010936:Plant Extracts / Q000494:pharmacology*; D020011:Protective Agents / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D020011:Protective Agents; D000432:Methanol