Apoptogenic peptides from Tityus discrepans scorpion venom acting against the SKBR3 breast cancer cell line.
Two novel peptides named neopladine 1 and neopladine 2 were purified from Tityus discrepans scorpion venom and found to be active on human breast carcinoma SKBR3 cells. Mass spectrometry molecular masses of neopladine 1 and 2 were 29918 and 30388 Da, respectively. Their N-terminal sequences were determined by Edman degradation. The peptides induced apoptosis of SKBR3 cells but had a negligible effect on non-malignant MA104 monkey kidney cells. Neopladine 1 and 2 induced 6.3 and 4.1% of SKBR3 apoptosis, respectively, in 5 h of exposure; the effect was larger with more prolonged exposures. Inmunohistochemistry showed that neopladines bind to SKBR3 cell surface inducing FasL and BcL-2 expression.
D'Suze
Gina
G
Laboratory on Cellular Neuropharmacology, Biophysics and Biochemistry Center, Instituto Venezolano de Investigaciones Cientificas (IVIC) Caracas, Venezuela. gina.dsuze@gmail.com
Rosales
Arnaldo
A
Salazar
Víctor
V
Sevcik
Carlos
C
D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000633:toxicity*; D017209:Apoptosis; D001943:Breast Neoplasms / Q000473:pathology; D045744:Cell Line, Tumor; D005591:Chemical Fractionation; D053222:Fas Ligand Protein / Q000378:metabolism; D005260:Female; D000882:Haplorhini; D006801:Humans; D007150:Immunohistochemistry; D010455:Peptides / Q000737:chemistry / Q000633:toxicity*; D012604:Scorpion Venoms / Q000737:chemistry* / Q000494:pharmacology; D020539:Sequence Analysis, Protein; D015398:Signal Transduction; D019014:fas Receptor / Q000378:metabolism
D000970:Antineoplastic Agents; D053222:Fas Ligand Protein; D010455:Peptides; D012604:Scorpion Venoms; D019014:fas Receptor