Adherence of Staphylococcus epidermidis to human endothelial cells is associated with a polysaccharidic component of its extracellular mucous layer.
Bacterial adherence to eukaryotic cells is highly contributing to microbial pathogenesis. Bacterial adhesins, macromolecules, and glycosaminoglycan chains of the endothelial cell surface have been implicated in staphylococcal attachment. Our research group has isolated an antigenic polysaccharidic component of Staphylococcus epidermidis extracellular layer, known as 20-kDa PS (PS), and showed that antibodies against this polysaccharide protect from infections. Therefore, the role of PS in S. epidermidis adherence to endothelial cells was studied. For this purpose we examined the impact of PS on the ability of two S. epidermidis strains (a PS-producing and a non-PS-producing strain) to adhere to human endothelial cells in the presence or absence of specific antibodies to this polysaccharide. Hence, it is established that exogenous chondroitin sulfate (CS) decreases, in part, the S. epidermidis' attachment to endothelial cells and the antagonistic binding effect of CS and PS was also studied. The results obtained demonstrate that PS facilitates the adherence of S. epidermidis to both strains. CS abolished the PS-induced adherence in PS-producing strain and partially in the non-PS-producing one. Conclusively, the adherence of S. epidermidis to human endothelial cells is associated with its extracellular PS component and it is suggested that the bacterial binding via glycosaminoglycan chains is an important mechanism underlining the PS-induced binding to endothelial cells.
Krevvata
Maria I
MI
Laboratory of Microbiology, School of Medicine, University of Patras, Greece.
Spiliopoulou
Anastasia
A
Anastassiou
Evangelos D
ED
Karamanos
Nikos
N
Kolonitsiou
Fevronia
F
D001422:Bacterial Adhesion / Q000187:drug effects*; D001710:Biotin / Q000378:metabolism; D002809:Chondroitin Sulfates / Q000494:pharmacology; D015169:Colony Count, Microbial; D042783:Endothelial Cells / Q000187:drug effects* / Q000382:microbiology*; D005110:Extracellular Space / Q000737:chemistry* / Q000187:drug effects; D006801:Humans; D009093:Mucus / Q000737:chemistry* / Q000187:drug effects; D011135:Polysaccharides, Bacterial / Q000494:pharmacology*; D012015:Reference Standards; D013194:Staining and Labeling; D013212:Staphylococcus epidermidis / Q000166:cytology* / Q000187:drug effects / Q000302:isolation & purification
D011135:Polysaccharides, Bacterial; D001710:Biotin; D002809:Chondroitin Sulfates