Comprehensive proteomics.
Extensive proteome discovery projects using a variety of mass spectrometric techniques have identified proteins matching to 50-70% of the predicted gene models of various species. Comprehensive proteome coverage is desirable for the unbiased comparison of protein quantities between different biological states and for the meaningful comparison of data from multiple samples. Here we discuss the feasibility of this goal in the light of recent technological developments.
Beck
Martin
M
European Molecular Biology Laboratory, Meyerhofstrasse 1, Heidelberg, Germany.
Claassen
Manfred
M
Aebersold
Ruedi
R
D000818:Animals; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods; D015870:Gene Expression; D006801:Humans; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods; D008432:Mathematical Computing; D008954:Models, Biological; D010446:Peptide Fragments / Q000032:analysis*; D011506:Proteins / Q000737:chemistry / Q000235:genetics; D020543:Proteome / Q000032:analysis* / Q000737:chemistry; D040901:Proteomics / Q000379:methods
D010446:Peptide Fragments; D011506:Proteins; D020543:Proteome