Molecular classification of an elasmobranch angiotensin receptor: quantification of angiotensin receptor and natriuretic peptide receptor mRNAs in saltwater and freshwater populations of the Atlantic stingray.
Among the most conserved osmoregulatory hormone systems in vertebrates are the renin-angiotensin system (RAS) and the natriuretic peptides (NPs). We examined the RAS and NP system in the euryhaline Atlantic stingray, Dasyatis sabina (Lesueur). To determine the relative sensitivity of target organs to these hormonal systems, we isolated cDNA sequences encoding the D. sabina angiotensin receptor (AT) and natriuretic peptide type-B receptor (NPR-B). We then determined the tissue-specific expression of their mRNAs in saltwater D. sabina from local Texas waters and an isolated freshwater population in Lake Monroe, Florida. AT mRNA was most abundant in interrenal tissue from both populations. NPR-B mRNA was most abundant in rectal gland tissue from both populations, and also highly abundant in the kidney of saltwater D. sabina. This study is the first to report the sequence of an elasmobranch angiotensin receptor, and phylogenetic analysis indicates that the D. sabina receptor is more similar to AT(1) vs. AT(2) proteins. This classification is further supported by molecular analysis of AT(1) and AT(2) proteins demonstrating conservation of AT(1)-specific amino acid residues and motifs in D. sabina AT. Molecular classification of the elasmobranch angiotensin receptor as an AT(1)-like protein provides fundamental insight into the evolution of the vertebrate RAS.
Evans
Andrew N
AN
The University of Texas Marine Science Institute, Port Aransas, Texas 78373, USA. evansan@mail.nih.gov
Henning
Toni
T
Gelsleichter
James
J
Nunez
B Scott
BS
D000595:Amino Acid Sequence; D000818:Animals; D018076:DNA, Complementary / Q000737:chemistry; D029941:Fish Proteins / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D005618:Fresh Water; D008969:Molecular Sequence Data; D010802:Phylogeny; D012333:RNA, Messenger / Q000378:metabolism; D011945:Receptors, Angiotensin / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D017461:Receptors, Atrial Natriuretic Factor / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D054712:Salinity; D016415:Sequence Alignment; D012860:Skates, Fish / Q000235:genetics* / Q000378:metabolism; D000099242:Atrial Natriuretic Factor Receptor B
D018076:DNA, Complementary; D029941:Fish Proteins; D012333:RNA, Messenger; D011945:Receptors, Angiotensin; D017461:Receptors, Atrial Natriuretic Factor; D000099242:Atrial Natriuretic Factor Receptor B