In vitro and in vivo gene delivery with tailor-designed aminofullerenes.
Long-range studies on the chemistry of two strained ring systems, cyclopropanes and fullerenes, converged on a challenge in biology, in vivo gene delivery. The new protocol of gene delivery employing a tailor-designed amino fullerene represents the third protocol after the two using virus and lipid delivery agents, none of which are universally useful. The amino fullerene delivers an EGFP and an insulin-2 gene to mice with efficiency superior to and selectivity distinct from those achieved by the conventional lipid-based delivery agents. The studies demonstrate the power of organic synthesis as driven by scientifically and socially important subjects.
Nakamura
Eiichi
E
Department of Chemistry, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan. nakamura@chem.s.u-tokyo.ac.jp
Isobe
Hiroyuki
H
D000818:Animals; D003521:Cyclopropanes / Q000737:chemistry; D004247:DNA / Q000737:chemistry; D037741:Fullerenes / Q000737:chemistry*; D018014:Gene Transfer Techniques; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D007328:Insulin / Q000235:genetics / Q000378:metabolism; D051379:Mice
D003521:Cyclopropanes; D037741:Fullerenes; D007328:Insulin; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D004247:DNA