title: Citrus fruit extracts reduce advanced glycation end products (AGEs)- and H₂O₂-induced oxidative stress in human adipocytes.
abstract: Diabetes is a reactive oxygen species (ROS)-mediated pathology, with a worldwide prevalence estimated to double by 2030. A major effort has been launched to find therapeutic means to improve health conditions of diabetic patients. Recent data show that supplemental natural antioxidants represent a potential strategy as adjunct therapy. Despite the major role of adipocytes in the etiology of diabetes, little is known about the effect of natural antioxidants on adipocyte response to oxidative stress. Using a diabetes-like oxidative stress model, the potential protective effect of antioxidative flavedo, albedo, and pulp extracts of (1) tangor Elendale (Citrus reticulata × Citrus sinensis) and (2) tangelo Minneola (C. reticulata × Citrus paradisis) was investigated on human adipocytes. Besides the retardation of free-radical-induced hemolysis of human erythrocytes, non-cytotoxic concentrations of tangelo and tangor flavedo extracts significantly reduced the levels of protein carbonyls in response to advanced glycation end products (AGEs) generated by albumin glycation in SW872 cells. Flavedo extracts lowered carbonyl accumulation in H2O2-treated adipocytes, while tangelo and tangor flavedo, albedo, and pulp extracts suppressed ROS production in SW872 cells with or without the addition of H2O2. Our results clearly show that Mauritian Citrus fruit extracts represent an important source of antioxidants, with a novel antioxidative role at the adipose tissue level.
authors.affiliation: Department of Agricultural and Food Science, Faculty of Agriculture, University of Mauritius, Réduit, Republic of Mauritius.
authors.forename: Deena
authors.initials: D
authors.lastname: Ramful
authors.forename: Evelyne
authors.initials: E
authors.lastname: Tarnus
authors.forename: Philippe
authors.initials: P
authors.lastname: Rondeau
authors.forename: Christine Robert
authors.initials: CR
authors.lastname: Da Silva
authors.forename: Theeshan
authors.initials: T
authors.lastname: Bahorun
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Bourdon
mesh_terms: D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism*; D000975:Antioxidants / Q000494:pharmacology; D002957:Citrus / Q000737:chemistry*; D015536:Down-Regulation / Q000187:drug effects; D005638:Fruit / Q000737:chemistry*; D017127:Glycation End Products, Advanced / Q000378:metabolism*; D006801:Humans; D006861:Hydrogen Peroxide / Q000009:adverse effects; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D017127:Glycation End Products, Advanced; D010936:Plant Extracts; D006861:Hydrogen Peroxide