title: Chilled storage of ram semen improves with the addition of egg yolk and glycerol to milk-based extenders.
abstract: This study aimed at comparing in vitro, ultra-heat-treated (UHT) skim milk and INRA-96(®) -based extenders supplemented or not with 5% egg yolk and/or 2% glycerol on sperm quality parameters along 72 h of preservation at 5 °C, using a factorial design. Semen from six healthy mature Merino rams was pooled and extended in each medium using a split sample procedure (six replicates) and chilled. Subjective motility (SM) (%), membrane integrity (MI) (%) and uncapacitated spermatozoa (US) (× 10(6) spermatozoa/AI dose) were used to assess the semen quality at 0, 12, 24, 48 and 72 h of preservation. UHT-based extenders yielded better (p<0.05) SM and MI than INRA-96(®) -based extenders (59.7% vs 57.9%; 60.2% vs 55.8%, respectively) but similar numbers of US (64.2% vs 62.3 × 10(6) sperm/AI dose, respectively) along the preservation time. Egg yolk-glycerol or just egg yolk as additives improved (p<0.05) the results compared with the base extenders without additives or just with glycerol. The sperm parameters assessed decline slowly from 0 to 48 h, with a sharp decline (p<0.05) at 72 h of preservation. In conclusion, UHT and INRA-96(®) were similar as base extenders, and the addition of 5% egg yolk plus 2% glycerol or just 5% egg yolk improved the quality of ram semen preserved at 5 °C, at least for 48 h. The combination of egg yolk-glycerol might provide extra protection in case of fluctuation of temperatures below 5 °C, commonly seen under field conditions.
authors.affiliation: Departamento de Salud en los Sistemas Pecuarios, Facultad de Veterinaria, Estación Experimental 'M. A. Cassinoni', Paysandú, Uruguay. jujogil@gmail.com
authors.forename: J
authors.initials: J
authors.lastname: Gil
authors.forename: S
authors.initials: S
authors.lastname: Fierro
authors.forename: O
authors.initials: O
authors.lastname: Bentancur
authors.forename: J
authors.initials: J
authors.lastname: Olivera-Muzante
mesh_terms: D000818:Animals; D003080:Cold Temperature; D003451:Cryoprotective Agents; D004530:Egg Yolk; D005990:Glycerol; D007315:Insemination, Artificial / Q000662:veterinary; D008297:Male; D008892:Milk; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D012756:Sheep; D013081:Sperm Motility; D013094:Spermatozoa / Q000502:physiology
chemical_list: D003451:Cryoprotective Agents; D005990:Glycerol