[Simultaneous determination of five lignans in Schisandra chinensis by HPLC].
OBJECTIVE
To develop a HPLC quantitative method for determination of schisandrin, schisandrol B, schisantherin A, deoxyschisandrin and gamma-schisandrin in Fructus Schisandrae.


METHODS
A symmetry C18 column (4.6 mm x 250 mm, 5 microm) was used with methanol (A) and water (B) as mobile phases, in gradient elution. The gradient program was as follows: 0-36 min, changed from 60% A to 66% A, 36-65 min, to 80% A, 65-70 min, kept for 80% A, 70-75 min, to 100% A. The flow rate was 0.5 mL x min(-1) and detection wavelength was set at 254 nm.


RESULTS
The linearities of schisandrin, schisandrol B, schisantherin A, deoxyschisandrin and gamma-schisandrin were in the ranges of 0.0214. 160 (r = 0.9999), 0.020-4.000 (r = 0.9999), 0.021-4.240 (r = 0.9999), 0.020-3.960 (r = 0.9999) and 0.021-4.200 (r = 0.9999). The average recoveries were 104.8%, 104.2%, 102.7%, 104.6%, 104.5%, respectively.


CONCLUSIONS
The method developed in this study was reliable, and can be used for the quality control of the fruits of S. chinensis.
Ding
Zuo
Z
College of Horticulture, Shenyang Agriculture University, Shenyang 110161, China. dingzuo.1985@163.com
Lu
Weiwei
W
Li
Hongbo
H
Fu
Junfan
J
Piao
Zhongyun
Z
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D017705:Lignans / Q000032:analysis* / Q000302:isolation & purification; D010936:Plant Extracts / Q000032:analysis* / Q000302:isolation & purification; D032141:Schisandra / Q000737:chemistry*
D017705:Lignans; D010936:Plant Extracts