Mycobacterium massiliense BRA100 strain recovered from postsurgical infections: resistance to high concentrations of glutaraldehyde and alternative solutions for high level disinfection.
OBJECTIVE
To evaluate the minimum inhibitory concentration (MIC) of GTA against these microorganisms and alternative disinfectants for high-level disinfection (HLD).


METHODS
Reference mycobacteria and clinical M. massiliense strains were included in this study. Active cultures were submitted to susceptibility qualitative tests with GTA dilutions (ranging from 1.5% to 8%), and commercial orthophthaldehyde (OPA) and peracetic acid (PA)-based solutions, during the period of exposure as recommended by National Agency of Sanitary Surveillance for HLD.


RESULTS
All reference and M. massiliense non-BRA100 strains, recovered from sputum, were susceptible to any GTA concentration, OPA and PA solutions. M. massiliense BRA100 strains presented MIC of 8% GTA and were susceptible to OPA and PA.


CONCLUSIONS
M. massiliense BRA100 strain is resistant to high GTA concentrations (up to 7%), which proves that this product is non-effective against specific rapidly growing mycobacteria and should be substituted by OPA or PA-based solutions for HLD.
Lorena
Nádia Suely de Oliveira
NS
Medical Sciences Faculty, Rio de Janeiro State University, Rio de Janeiro, Brazil.
Pitombo
Marcos Bettini
MB
Côrtes
Patrícia Barbur
PB
Maya
Maria Cristina Araújo
MC
Silva
Marlei Gomes da
MG
Carvalho
Ana Carolina da Silva
AC
Coelho
Fábrice Santana
FS
Miyazaki
Neide Hiromi Tokumaru
NH
Marques
Elizabeth Andrade
EA
Chebabo
Alberto
A
Freitas
Andréa D'Avila
AD
Lupi
Otília
O
Duarte
Rafael Silva
RS
D000447:Aldehydes / Q000494:pharmacology*; D004202:Disinfectants / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial / Q000187:drug effects*; D005976:Glutaral / Q000008:administration & dosage / Q000494:pharmacology*; D006801:Humans; D008826:Microbial Sensitivity Tests; D009161:Mycobacterium / Q000145:classification / Q000187:drug effects* / Q000302:isolation & purification; D010463:Peracetic Acid / Q000494:pharmacology*; D011183:Postoperative Complications / Q000382:microbiology
D000447:Aldehydes; D004202:Disinfectants; D010463:Peracetic Acid; D005976:Glutaral