[Interleukin-1beta induces MUC2/MUC5B gene expression in human nasal epithelial cells].
OBJECTIVE
To demonstrate the effects of IL-1beta on MUC2/MUC5B gene expression in cultured human nasal epithelial cells.


METHODS
In passage-2 cultured human nasal epithelial cells, the mRNA levels of MUC2/MUC5B gene expression induced by IL-1 beta were determined by fluorescent quantitative RT-PCR.


RESULTS
MUC2/MUC5B mRNAs were detected after 24 h of exposure to IL-1beta. MUC2 mRNA levels in IL-1 beta treatment [(39.26 +/- 6.10) x 10(4) copy/microg] were significantly higher than control [(5.70 +/- 4.16) x 10(4) copy/microg] (P < 0.01). MUC5BmRNA levels in IL-1beta treatment [(5.7 +/- 2.06) x 10(5) copy/microg] were significantly higher than control [(1.11 +/- 0.72) 10(5) copy/microg] (P < 0.05).


CONCLUSIONS
IL-1 beta increased MUC2/MUC5B mRNA levels in human nasal epithelial cells. These results suggest that IL-1beta may enhance mucin gene expression in cultured human nasal epithelial cells.
Huang
Xuekun
X
Department of Otorhinolaryngology, the Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, 510630, China.
Li
Yuan
Y
Wang
Tao
T
Li
Peng
P
Liu
Xian
X
Liu
Hong
H
Xu
Wiejie
W
D002478:Cells, Cultured; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D053583:Interleukin-1beta / Q000494:pharmacology*; D055262:Mucin-2 / Q000235:genetics / Q000378:metabolism*; D055257:Mucin-5B / Q000235:genetics / Q000378:metabolism*; D009297:Nasal Mucosa / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D015854:Up-Regulation
D053583:Interleukin-1beta; C524625:MUC2 protein, human; C524578:MUC5B protein, human; D055262:Mucin-2; D055257:Mucin-5B; D012333:RNA, Messenger