Functional protein microarray technology.
Functional protein microarrays are emerging as a promising new tool for large-scale and high-throughput studies. In this article, we review their applications in basic proteomics research, where various types of assays have been developed to probe binding activities to other biomolecules, such as proteins, DNA, RNA, small molecules, and glycans. We also report recent progress of using functional protein microarrays in profiling protein post-translational modifications, including phosphorylation, ubiquitylation, acetylation, and nitrosylation. Finally, we discuss potential of functional protein microarrays in biomarker identification and clinical diagnostics. We strongly believe that functional protein microarrays will soon become an indispensible and invaluable tool in proteomics research and systems biology.
Hu
Shaohui
S
Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Xie
Zhi
Z
Qian
Jiang
J
Blackshaw
Seth
S
Zhu
Heng
H
D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D006801:Humans; D040081:Protein Array Analysis / Q000379:methods* / Q000639:trends; D011499:Protein Processing, Post-Translational; D040901:Proteomics / Q000379:methods* / Q000639:trends; D049490:Systems Biology / Q000379:methods* / Q000639:trends
D015415:Biomarkers