title: The dependence of the incorporation of methamphetamine into rat hair on dose, frequency of administration and hair pigmentation.
abstract: In this paper, the incorporation of methamphetamine (MA) into rat hair was studied. The main purpose of this study was to investigate whether MA can be detected or positive hair results can be obtained in hair of rats administered a single dose of MA. The relationship between dose and frequency of administration and the concentrations of MA and its metabolite, amphetamine (AP), in rat hair were evaluated and the MA and AP concentrations in white and pigmented hair were compared. MA was administered to rats as follows: low dose (0.5mg/kg/day), medium dose (2mg/kg/day) and high dose (10mg/kg/day). The frequency of administration was one time per day for 1, 2, 3, 4, 5, 15 and 30 days. Hair and urine samples were collected from rats and analyzed by gas chromatography/mass spectrometry (GC/MS). MA could be identified in pigmented rat hair when MA was administered for 4 or more days at low daily dose and on day 1 following administration of medium and high daily doses. Positive results for MA were obtained from pigmented rat hair when MA was administered for 30 days at low daily dose, for 4 or more days at medium daily dose, or for 2 or more days at high daily dose. The concentrations of MA and AP found in rat hair were proportional to the dose and frequency of administration. The concentrations of MA and AP in pigmented rat hair were 2-10 times higher than those in white rat hair. The results of this study on the incorporation of MA into rat hair can serve as a model to better understand the incorporation of MA into human hair even though there are differences between animal models and human hair.
authors.affiliation: National Institute of Scientific Investigation, 331-1 Shinwol 7-Dong, Yang-Chun Gu, Seoul, South Korea.
authors.forename: Eunyoung
authors.initials: E
authors.lastname: Han
authors.forename: Yonghoon
authors.initials: Y
authors.lastname: Park
authors.forename: Eunmi
authors.initials: E
authors.lastname: Kim
authors.forename: Sooyeun
authors.initials: S
authors.lastname: Lee
authors.forename: Hwakyung
authors.initials: H
authors.lastname: Choi
authors.forename: Heesun
authors.initials: H
authors.lastname: Chung
authors.forename: Joon Myong
authors.initials: JM
authors.lastname: Song
mesh_terms: D000661:Amphetamine / Q000032:analysis / Q000652:urine; D000818:Animals; D004305:Dose-Response Relationship, Drug; D008401:Gas Chromatography-Mass Spectrometry; D006197:Hair / Q000737:chemistry / Q000378:metabolism*; D016014:Linear Models; D008297:Male; D008694:Methamphetamine / Q000008:administration & dosage* / Q000032:analysis / Q000493:pharmacokinetics* / Q000652:urine; D023421:Models, Animal; D010858:Pigmentation; D051381:Rats; D011924:Rats, Zucker; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D008694:Methamphetamine; D000661:Amphetamine