MKP1 regulates the induction of inflammatory response by pneumococcal pneumolysin in human epithelial cells.
The expression of proinflammatory cytokines represents an important host innate response during infections. The reduction of cytokine expression thus mediates impaired host defenses. We previously reported that pneumococcal pneumolysin is less potent in inducing inflammatory responses in human epithelial cells at the early stage of treatment. How this might occur in response to pneumolysin is still not clearly understood. Here, we show the expression of tumor necrosis factor-α (TNF-α) was reduced by MAPK phosphatase 1 (MKP1), expression of which was significantly increased in response to pneumolysin at the early stage of treatment. TNF-α expression was mediated in a time-dependent manner by p38 mitogen-activated protein kinase, activation of which is under the control of MKP1. Thus, this study reveals novel roles of pneumolysin in mediating MKP1 expression for the regulation of proinflammatory cytokine expression in a time-dependent manner.
Shin
Hee-Sung
HS
Department of Biotechnology and Bioinformatics, Korea University, Chungnam, Korea.
Yoo
In-Hwa
IH
Kim
Yong-Jae
YJ
Kim
Hyong-Bai
HB
Jin
Shouguang
S
Ha
Un-Hwan
UH
D001426:Bacterial Proteins / Q000276:immunology / Q000378:metabolism; D026481:Blotting, Far-Western; D002460:Cell Line; D045744:Cell Line, Tumor; D016207:Cytokines / Q000378:metabolism; D054638:Dual Specificity Phosphatase 1 / Q000378:metabolism*; D004847:Epithelial Cells / Q000276:immunology* / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D007249:Inflammation / Q000276:immunology*; D011008:Pneumococcal Infections / Q000382:microbiology; D016133:Polymerase Chain Reaction; D015398:Signal Transduction / Q000187:drug effects; D013296:Streptococcus pneumoniae / Q000276:immunology* / Q000472:pathogenicity; D013301:Streptolysins / Q000276:immunology* / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis* / Q000276:immunology; D037521:Virulence Factors / Q000276:immunology / Q000378:metabolism; D048051:p38 Mitogen-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism
D001426:Bacterial Proteins; D016207:Cytokines; D013301:Streptolysins; D014409:Tumor Necrosis Factor-alpha; D037521:Virulence Factors; C032290:plY protein, Streptococcus pneumoniae; D048051:p38 Mitogen-Activated Protein Kinases; C517019:DUSP1 protein, human; D054638:Dual Specificity Phosphatase 1