RGS6/Gβ5 complex accelerates IKACh gating kinetics in atrial myocytes and modulates parasympathetic regulation of heart rate.
BACKGROUND
The parasympathetic reduction in heart rate involves the sequential activation of m2 muscarinic cholinergic receptors (m(2)Rs), pertussis toxin-sensitive (Gi/o) heterotrimeric G proteins, and the atrial potassium channel I(KACh). Molecular mechanisms regulating this critical signal transduction pathway are not fully understood.


OBJECTIVE
To determine whether the G protein signaling regulator Rgs6/Gβ5 modulates m(2)R-I(KACh) signaling and cardiac physiology.


RESULTS
Cardiac expression of Rgs6, and its interaction with Gβ5, was demonstrated by immunoblotting and immunoprecipitation. Rgs6(-/-) mice were generated by gene targeting, and the cardiac effects of Rgs6 ablation were analyzed by whole-cell recordings in isolated cardiomyocytes and ECG telemetry. Loss of Rgs6 yielded profound delays in m(2)R-I(KACh) deactivation kinetics in both neonatal atrial myocytes and adult sinoatrial nodal cells. Rgs6(-/-) mice exhibited mild resting bradycardia and altered heart rate responses to pharmacological manipulations that were consistent with enhanced m(2)R-I(KACh) signaling.


CONCLUSIONS
The cardiac Rgs6/Gβ5 complex modulates the timing of parasympathetic influence on atrial myocytes and heart rate in mice.
Posokhova
Ekaterina
E
Department of Pharmacology, University of Minnesota, Minneapolis, MN 55455, USA.
Wydeven
Nicole
N
Allen
Kevin L
KL
Wickman
Kevin
K
Martemyanov
Kirill A
KA
D000818:Animals; D015536:Down-Regulation / Q000235:genetics / Q000502:physiology; D051676:G Protein-Coupled Inwardly-Rectifying Potassium Channels / Q000502:physiology; D044387:GTP-Binding Protein beta Subunits / Q000502:physiology*; D057809:HEK293 Cells; D006325:Heart Atria / Q000166:cytology / Q000503:physiopathology; D006339:Heart Rate / Q000235:genetics / Q000502:physiology*; D006801:Humans; D015640:Ion Channel Gating / Q000235:genetics / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D032383:Myocytes, Cardiac / Q000502:physiology*; D017777:Parasympathetic Fibers, Postganglionic / Q000502:physiology*; D024642:Potassium Channels, Voltage-Gated / Q000037:antagonists & inhibitors / Q000502:physiology*; D020710:RGS Proteins / Q000502:physiology*; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology; D015854:Up-Regulation / Q000235:genetics / Q000502:physiology*
D051676:G Protein-Coupled Inwardly-Rectifying Potassium Channels; D044387:GTP-Binding Protein beta Subunits; C094668:Gnb5 protein, mouse; D024642:Potassium Channels, Voltage-Gated; D020710:RGS Proteins; C485692:Rgs6 protein, mouse; C059983:potassium channel protein I(sk)