title: Identification of apolipoprotein C-I in rainbow trout seminal plasma.
abstract: Three distinct bands with high electrophoretic migration rates were isolated and purified from rainbow trout seminal plasma. The molecular masses of these bands were determined to be 5158.8, 4065.9 and 4929.0 Da. The N-terminal amino acids sequences were elucidated and were found to have high homology with Atlantic salmon apolipoprotein C-I. It can be concluded that apolipoprotein C-I is a major component of rainbow trout seminal plasma. Further studies are necessary to confirm the protective effects of apolipoprotein C-I on spermatozoa in terms of the stabilisation of the sperm membrane.
authors.affiliation: Department of Gamete and Embryo Biology, Polish Academy of Sciences, Tuwima 10, 10-747 Olsztyn, Poland. nynec@pan.olsztyn.pl
authors.forename: Joanna
authors.initials: J
authors.lastname: Nynca
authors.forename: Mariola A
authors.initials: MA
authors.lastname: Dietrich
authors.forename: Halina
authors.initials: H
authors.lastname: Karol
authors.forename: Andrzej
authors.initials: A
authors.lastname: Ciereszko
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D053302:Apolipoprotein C-I / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D056148:Chromatography, Reverse-Phase; D004591:Electrophoresis, Polyacrylamide Gel; D029941:Fish Proteins / Q000737:chemistry / Q000302:isolation & purification*; D008297:Male; D008969:Molecular Sequence Data; D008970:Molecular Weight; D017686:Oncorhynchus mykiss / Q000378:metabolism*; D012661:Semen / Q000737:chemistry*; D017386:Sequence Homology, Amino Acid
chemical_list: D053302:Apolipoprotein C-I; D029941:Fish Proteins