title: The use of cytokine array to examine cytokine profiles of two human cell lines exposed to indoor dust.
abstract: Human cytokine array was used to investigate the cytokine profile of U937 and KERTr after exposure to indoor dust or dust extracts. The release of MCP-1 was increased while release of IL-8 and IL-1β on U937 were decreased after exposure to indoor dust. The releases of RANTES, IL-8 and VEGF from KERTr after exposure to dust extract were increased. The results of IL-8 ELISA assay were consistent with the cytokine array. Real-time RT-PCR was performed to analyze relative changes in gene expression. The MCP-1 mRNA levels were increased after U937 exposure to 18 indoor dust samples, whereas, IL-8 and IL-1β mRNA level showed both up-regulation and down-regulation. The dose-related increase and decrease response was observed on MCP-1 and IL-8, respectively. Most indoor dust extracts increased RANTES, IL-8 and VEGF mRNA levels on KERTr. The dose-dependent response was observed on RANTES and IL-8. A significant correlation (r=0.48, p<0.05) was obtained between the total PAHs concentration in dust extracts and the induction of RANTES mRNA.
authors.affiliation: Croucher Institute for Environmental Sciences, Department of Biology, Hong Kong Baptist University, Kowloon Tong, Hong Kong, PR China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Kang
authors.forename: Kwai Chung
authors.initials: KC
authors.lastname: Cheung
authors.forename: Ming H
authors.initials: MH
authors.lastname: Wong
mesh_terms: D016902:Air Pollution, Indoor / Q000009:adverse effects*; D016207:Cytokines / Q000032:analysis* / Q000235:genetics; D004391:Dust; D004797:Enzyme-Linked Immunosorbent Assay; D005786:Gene Expression Regulation; D006801:Humans; D015603:Keratinocytes / Q000276:immunology; D012333:RNA, Messenger / Q000032:analysis; D013172:Spores, Fungal; D020298:U937 Cells
chemical_list: D016207:Cytokines; D004391:Dust; D012333:RNA, Messenger