title: Purification, characterization and comparison of phycoerythrins from three different marine cyanobacterial cultures.
abstract: The present study is focused on purification, characterization and comparison of phycoerythrins from three different marine cyanobacterial cultures--hormidium sp. A27 DM, Lyngbya sp. A09 DM and Halomicronema sp. A32 DM. 'Phycoerythrin' was successfully purified and characterized. On SDS-PAGE, the PE purified from all three young cultures showed four bands--corresponding to α and β subunits of each of PE-I and PE-II. However, phycoerythrin purified after prolonged growth of Phormidium sp. A27 DM and Halomicronema sp. A32DM showed only one band corresponding to 14 kDa whereas Lyngbya sp. A09 DM continued to produce uncleaved phycoerythrin. The absorption spectra of purified PEs from all the three young and old cultures showed variations however the fluorescence studies of the purified PEs in all cases gave the emission spectra at around 580 nm. The described work is of great importance to understand the role of phycoerythrin in adapting cyanobacteria to stress conditions.
authors.affiliation: BRD School of Biosciences, Sardar Patel Maidan, Vadtal Road, Satellite Campus, Post Box No. 39, Sardar Patel University, Vallabh Vidyanagar 388 120, Anand, Gujarat, India. parmar.asha@gmail.com
authors.forename: Asha
authors.initials: A
authors.lastname: Parmar
authors.forename: Niraj Kumar
authors.initials: NK
authors.lastname: Singh
authors.forename: Avani
authors.initials: A
authors.lastname: Kaushal
authors.forename: Sagar
authors.initials: S
authors.lastname: Sonawala
authors.forename: Datta
authors.initials: D
authors.lastname: Madamwar
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D000458:Cyanobacteria / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D010799:Phycoerythrin / Q000302:isolation & purification* / Q000378:metabolism*; D012623:Seawater / Q000382:microbiology*; D016622:Silver Staining; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
chemical_list: D001426:Bacterial Proteins; D010799:Phycoerythrin