title: IL6 -174 genotype associated with Aggregatibacter actinomycetemcomitans in Indians.
abstract: OBJECTIVE
  Genetic factors have recently been associated with presence of Aggregatibacter actinomycetemcomitans subgingivally in populations living in industrialized countries. The aim of this study was to analyse associations between Interleukin-6 (IL6) single nucleotide polymorphisms and presence and levels of A. actinomycetemcomitans and other subgingival microbes in a rural Indian population.
  
  
  METHODS
  A total of 251 individuals from a rural village in India with a periodontal phenotype ranging from healthy to severe periodontitis were included. Checkerboard DNA-DNA analysis was performed to detect 40 periodontal taxa in subgingival plaque samples. Genomic DNA was extracted to genotype five polymorphisms in the IL6 promoter region.
  
  
  RESULTS
  The IL6-74 GG genotype was associated with high (above median) counts of A. actinomycetemcomitans (both in all subjects and in periodontally healthy only) and with presence and counts of Capnocytophaga sputigena. Differences in detection of several other bacteria were noted between periodontitis and healthy subjects.
  
  
  CONCLUSIONS
  These findings support the influence of genetic factors on the subgingival microbiota.
authors.affiliation: Periodontology Unit and Division of Clinical Research, Eastman Dental Hospital, University College London (UCL), London, UK. l.nibali@eastman.ucl.ac.uk
authors.forename: L
authors.initials: L
authors.lastname: Nibali
authors.forename: I
authors.initials: I
authors.lastname: Madden
authors.forename: F
authors.initials: F
authors.lastname: Franch Chillida
authors.forename: Lja
authors.initials: L
authors.lastname: Heitz-Mayfield
authors.forename: Pm
authors.initials: P
authors.lastname: Brett
authors.forename: N
authors.initials: N
authors.lastname: Donos
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D016976:Aggregatibacter actinomycetemcomitans / Q000302:isolation & purification*; D058491:Bacterial Load; D001439:Bacteroides / Q000302:isolation & purification; D044883:Campylobacter rectus / Q000302:isolation & purification; D002206:Capnocytophaga / Q000302:isolation & purification; D003596:Cytosine; D003773:Dental Plaque / Q000382:microbiology; D014644:Genetic Variation / Q000235:genetics; D005838:Genotype; D005881:Gingiva / Q000382:microbiology*; D006147:Guanine; D006801:Humans; D007194:India; D015850:Interleukin-6 / Q000235:genetics*; D008875:Middle Aged; D017622:Periodontal Attachment Loss / Q000145:classification / Q000382:microbiology; D010514:Periodontal Pocket / Q000145:classification / Q000382:microbiology; D010518:Periodontitis / Q000235:genetics / Q000382:microbiology; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D016966:Porphyromonas gingivalis / Q000302:isolation & purification; D018713:Prevotella intermedia / Q000302:isolation & purification; D011401:Promoter Regions, Genetic / Q000235:genetics; D012423:Rural Health; D048373:Treponema denticola / Q000302:isolation & purification; D055815:Young Adult
chemical_list: D015850:Interleukin-6; D006147:Guanine; D003596:Cytosine