title: Accelerated stem cell attachment to ultrafine grained titanium.
abstract: Commercial purity titanium with an average grain size in the low sub-micron range was produced by equal channel angular pressing (ECAP). Attachment of human bone marrow-derived mesenchymal stem cells (hMSCs) to the surface of conventional coarse grained and ECAP-modified titanium was studied. It was demonstrated that the attachment and spreading of hMSCs in the initial stages (up to 24h) of culture was enhanced by grain refinement. Surface characterization by a range of techniques showed that the main factor responsible for the observed acceleration of hMSC attachment and spreading on titanium due to grain refinement in the bulk is the attendant changes in surface topography on the nanoscale. These results indicate that, in addition to its superior mechanical properties, ECAP-modified titanium possesses improved biocompatibility, which makes it to a potent candidate for applications in medical implants.
authors.affiliation: ARC Centre of Excellence for Design in Light Metals, Department of Materials Engineering, Monash University, Clayton, Victoria 3800, Australia. yuri.estrin@monash.edu
authors.forename: Yuri
authors.initials: Y
authors.lastname: Estrin
authors.forename: Elena P
authors.initials: EP
authors.lastname: Ivanova
authors.forename: Anna
authors.initials: A
authors.lastname: Michalska
authors.forename: Vi Khanh
authors.initials: VK
authors.lastname: Truong
authors.forename: Rimma
authors.initials: R
authors.lastname: Lapovok
authors.forename: Richard
authors.initials: R
authors.lastname: Boyd
mesh_terms: D001854:Bone Marrow Cells / Q000166:cytology / Q000648:ultrastructure; D002448:Cell Adhesion / Q000187:drug effects; D048429:Cell Size / Q000187:drug effects; D004602:Elements; D005898:Glass; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects* / Q000648:ultrastructure; D018625:Microscopy, Atomic Force; D018613:Microscopy, Confocal; D010316:Particle Size; D013499:Surface Properties / Q000187:drug effects; D014025:Titanium / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D004602:Elements; D014025:Titanium