Immmunological clearance of Batrachochytrium dendrobatidis infection at a pathogen-optimal temperature in the hylid frog Hypsiboas crepitans.
Amphibian declines worldwide have been linked to the fungal disease chytridiomycosis. Its causative agent (Batrachochytrium dendrobatidis, hereafter Bd), however, also infects many nondeclining species. Experimental infections have shown species-specific and temperature-dependent frog responses to Bd infection. Although Bd infection may be eliminated by housing amphibians at temperatures above those tolerated by the fungus, the question of whether frogs can eliminate infection under more favorable conditions remains unanswered. Repeated diagnostics using real-time polymerase chain reaction (rt-PCR) assays of postmetamorphic individuals at 28, 38, 45, 53, and 62 days after exposure demonstrated that Hypsiboas crepitans is able to clear infection within a few weeks at 23°C. Thus, we demonstrate a temperature-independent and likely immunological mechanism for the clearance of Bd in a resistant amphibian species. Future studies are needed to determine the generality of this mechanism among amphibians and to describe the immune factors affecting different outcomes of Bd exposure including resistance to infection, tolerance of infection, and clearance of infection.
Márquez
M
M
Centro de Ecología, Instituto Venezolano de Investigaciones Científicas, Caracas, Venezuela.
Nava-González
F
F
Sánchez
D
D
Calcagno
M
M
Lampo
M
M
D000818:Animals; D001001:Anura / Q000276:immunology* / Q000382:microbiology*; D008411:Chytridiomycota / Q000276:immunology* / Q000302:isolation & purification; D009181:Mycoses / Q000175:diagnosis / Q000662:veterinary*; D016133:Polymerase Chain Reaction; D012867:Skin / Q000382:microbiology; D013048:Specimen Handling; D013696:Temperature; D013997:Time Factors; D014687:Venezuela