Lack of association of C599T polymorphism in the glutathione peroxidase (GPX1) gene with delayed graft function, acute kidney graft rejection and chronic allograft nephropathy.
BACKGROUND
The glutathione peroxidase/glutathione system is a major defense in oxidative stress. Glutathione peroxidase (GPx) is a selenium-containing antioxidant enzyme that effectively reduces hydrogen peroxide and lipid peroxides to water and lipid alcohols, respectively, and in turn oxidizes glutathione to glutathione disulfide. Previous studies have shown that the activity of glutathione peroxidase is genetically determined and is associated with polymorphisms in GPX1 gene. The aim of the present study was to examine the association between the C599T polymorphism in the glutathione peroxidase (GPX1) gene and delayed graft function of kidney allografts, acute rejection and chronic allograft nephropathy.


METHODS
One hundred eighty-seven recipients of first cadaveric renal transplants from the Department of Nephrology, Transplantology and Internal Medicine of Pomeranian Medical University were included in this retrospective study. Genotyping of C599T polymorphism in the GPX1 gene was performed using PCR-RFLP method.


RESULTS
There were no significant associations between this polymorphism and delayed graft function, acute rejection and chronic allograft nephropathy.


CONCLUSIONS
The present results suggest that GPX1 C599T polymorphism has no influence on the graft function in the first phase after transplantation, as well as on the acute kidney graft rejection and chronic allograft nephropathy.
Dutkiewicz
Grazyna
G
Department of Nephrology, Transplantology and Internal Medicine of Pomeranian Medical University, Szczecin, Poland.
Binczak-Kuleta
Agnieszka
A
Pawlik
Andrzej
A
Safranow
Krzysztof
K
Wisniewska
Magda
M
Ciechanowicz
Andrzej
A
Dziedziejko
Violetta
V
Ciechanowski
Kazimierz
K
Domanski
Leszek
L
D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D000368:Aged; D000975:Antioxidants / Q000378:metabolism; D002908:Chronic Disease; D051799:Delayed Graft Function / Q000235:genetics*; D005260:Female; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000235:genetics* / Q000378:metabolism; D006084:Graft Rejection / Q000235:genetics*; D006801:Humans; D007668:Kidney / Q000378:metabolism; D007674:Kidney Diseases / Q000473:pathology; D016030:Kidney Transplantation / Q000009:adverse effects; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress / Q000235:genetics; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D012189:Retrospective Studies; D012643:Selenium / Q000378:metabolism; D055815:Young Adult
D000975:Antioxidants; D005979:Glutathione Peroxidase; D005978:Glutathione; D012643:Selenium