Stringent programming of DNA methylation in humans.
We describe a PCR-based method called Amplified Methylation Polymorphism (AMP) for scanning genomes for DNA methylation changes. AMP detects tissue-specific DNA methylation signatures often representing junctions between methylated and unmethylated DNA close to intronexon junctions and/or associated with CpG islands. Identical AMP profiles are detected for healthy, young, monozygotic twins.
Aung
Hnin T
HT
School of Chemistry and Molecular Biosciences, The University of Queensland, Australia.
Harrison
Dion K
DK
Findlay
Ian
I
Mattick
John S
JS
Martin
Nicholas G
NG
Carroll
Bernard J
BJ
D000293:Adolescent; D000818:Animals; D001483:Base Sequence; D018899:CpG Islands; D019175:DNA Methylation / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D005091:Exons; D005819:Genetic Markers; D006801:Humans; D007438:Introns; D051379:Mice; D021141:Nucleic Acid Amplification Techniques / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D014430:Twins, Monozygotic / Q000235:genetics*
D017931:DNA Primers; D005819:Genetic Markers