Tobacco microtubule-associated protein, MAP65-1c, bundles and stabilizes microtubules.
Three genes that encode MAP65-1 family proteins have been identified in the Nicotiana tabacum genome. In this study, NtMAP65-1c fusion protein was shown to bind and bundle microtubules (MTs). Further in vitro investigations demonstrated that NtMAP65-1c not only alters MT assembly and nucleation, but also exhibits high MT stabilizing activity against cold or katanin-induced destabilization. Analysis of NtMAP65-1c-GFP expressing BY-2 cells clearly demonstrated that NtMAP65-1c was able to bind to MTs during specific stages of the cell cycle. Furthermore, in vivo, NtMAP65-1c-GFP-bound cortical MTs displayed an increase in resistance against the MT-disrupting drug, propyzamide, as well as against cold temperatures. Taken together, these results strongly suggest that NtMAP65-1c stabilizes MTs and is involved in the regulation of MT organization and cellular dynamics.
Meng
Qiutao
Q
State Key Laboratory of Plant Physiology and Biochemistry, Department of Plant Sciences, College of Biological Sciences, China Agricultural University, Beijing, 100193, China.
Du
Jizhou
J
Li
Jiejie
J
Lü
Xiaomei
X
Zeng
Xian
X
Yuan
Ming
M
Mao
Tonglin
T
D049452:Green Fluorescent Proteins / Q000032:analysis; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D008870:Microtubules / Q000378:metabolism* / Q000648:ultrastructure; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D058105:Polymerization; D055550:Protein Stability; D011993:Recombinant Fusion Proteins / Q000032:analysis; D014026:Nicotiana / Q000378:metabolism* / Q000648:ultrastructure
D008869:Microtubule-Associated Proteins; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins