A chimeric peptide that binds to titanium and mediates MC3T3-E1 cell adhesion.
Our study provides a promising alternative of biomimetic coating which functionalizes the dental implant with adhesion peptides and may be useful for enhancing the bone remodeling around Ti implants. A chimeric peptide consisting of an Arg-Gly-Asp (RGD) sequence (mediating cell adhesion) and a RKLPDA (minTBP-1) sequence (specifically recognizing and binding to Ti substrate) was designed and synthesized. The chimeric peptide affinity to Ti disks, as well as its role in mediating MC3T3-E1 cell attachment and afterwards spreading on pre-coated Ti disks, was investigated. The chimeric peptide not only showed favorable affinity to Ti surfaces but also facilitated the adhesion of MC3T3-E1 cells.
Wang
Dan
D
Department of Stomatology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, No. 1095, Jiefang Road, Hankou District, Wuhan, 430030, Hubei, People's Republic of China.
Mao
Jing
J
Zhou
Bin
B
Liao
Xiao-Fu
XF
Gong
Shi-Qiang
SQ
Liu
Yan
Y
Zhang
Jing-Tao
JT
D000818:Animals; D040761:Biomimetic Materials / Q000378:metabolism*; D002448:Cell Adhesion; D002460:Cell Line; D020099:Coated Materials, Biocompatible / Q000378:metabolism; D051379:Mice; D010006:Osteoblasts / Q000502:physiology*; D011485:Protein Binding; D011994:Recombinant Proteins / Q000378:metabolism*; D014025:Titanium / Q000378:metabolism*
D020099:Coated Materials, Biocompatible; D011994:Recombinant Proteins; D014025:Titanium