Injectable hyaluronic acid-tyramine hydrogels for the treatment of rheumatoid arthritis.
Rheumatoid arthritis (RA) is a chronic inflammatory disease caused by inflammation of the synovial membrane, leading in turn to articular cartilage destruction. In this work, injectable tyramine modified hyaluronic acid (HA-Tyr) hydrogels were developed for the treatment of RA. HA-Tyr conjugate was synthesized by amide bond formation between carboxyl groups of HA and amine groups of tyramine. Then, HA-Tyr hydrogels were prepared by radical crosslinking reaction using H(2)O(2) and horse-radish peroxidase. Intra-articular injection of HA-Tyr hydrogels encapsulating dexamethasone (DMT) as a model drug resulted in successful treatment of RA with reduced interlukine-6, prostaglandin E2 and four types of cytokine levels in collagen-induced arthritis animal models. Histological analysis with hematoxylin and eosin (H&E) staining also confirmed the therapeutic effect of injectable HA-Tyr hydrogels with DMT. Taken together, the injectable HA-Tyr hydrogels were thought suitable to be developed as a therapeutically effective drug carrier for the treatment of RA.
Kim
K S
KS
Department of Materials Science and Engineering, Pohang University of Science and Technology, San 31, Hyoja-dong, Nam-gu, Pohang, Kyungbuk 790-784, Republic of Korea.
Park
S J
SJ
Yang
J-A
JA
Jeon
J-H
JH
Bhang
S H
SH
Kim
B-S
BS
Hahn
S K
SK
D000818:Animals; D001169:Arthritis, Experimental / Q000097:blood / Q000188:drug therapy* / Q000473:pathology; D001172:Arthritis, Rheumatoid / Q000097:blood / Q000188:drug therapy* / Q000473:pathology; D015153:Blotting, Western; D002645:Chickens; D003907:Dexamethasone / Q000627:therapeutic use; D015232:Dinoprostone / Q000097:blood; D006820:Hyaluronic Acid / Q000138:chemical synthesis / Q000737:chemistry*; D020100:Hydrogels / Q000138:chemical synthesis / Q000737:chemistry*; D007267:Injections; D015850:Interleukin-6 / Q000097:blood; D007596:Joints / Q000473:pathology; D009682:Magnetic Resonance Spectroscopy; D051381:Rats; D017207:Rats, Sprague-Dawley; D014439:Tyramine / Q000138:chemical synthesis / Q000737:chemistry*
D020100:Hydrogels; D015850:Interleukin-6; D003907:Dexamethasone; D006820:Hyaluronic Acid; D015232:Dinoprostone; D014439:Tyramine