A novel electrochemical method to detect cell surface carbohydrates and target cells.
Glycosylation of cell surfaces is a critical factor in many biological processes; however, the lack of effective analytical tools for the detection of cell surface carbohydrates has been the bottleneck for probing into the processes. In this paper, a novel electrochemical method is presented for the analysis of cell surface carbohydrates, which can be also used to detect the target cells. Firstly, 5-hydroxy-3-hexanedithiol-1,4-naphthoquinone (JUG(thio)), the electrochemical reporter, and anti-selectin aptamer are successively modified onto the surface of a gold electrode. Different concentrations of intestinal human colon adenocarcinoma (LS180) cells are employed as the target cells for this study. Consequently, the specific carbohydrates on the surfaces of LS180 cells and anti-selectin aptamers will compete for combination with selectin in the system. As a result, the oxidation signal of JUG(thio) is changed and the detection of the cell surface carbohydrates can be achieved easily and sensitively. Furthermore, the proposed method can be used to specifically detect LS180 cells in a wide concentration range, from 10(3) to 10(7) cells/mL, with a good linear relationship and low detection limit, which might be promising for the diagnosis of cancer and some other diseases in the future.
Shao
Zhenyu
Z
Laboratory of Biosensing Technology, School of Life Science, Shanghai University, Shanghai 200444, China.
Li
Yun
Y
Yang
Qianlu
Q
Wang
Jing
J
Li
Genxi
G
D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D045744:Cell Line, Tumor; D055664:Electrochemical Techniques / Q000379:methods*; D006801:Humans; D019041:L-Selectin / Q000032:analysis*; D009285:Naphthoquinones / Q000737:chemistry*
D052157:Aptamers, Nucleotide; D009285:Naphthoquinones; D019041:L-Selectin; C035342:1,4-naphthoquinone