Mucosal adenovirus-vectored vaccine for measles.
Several problems associated with the available anti-measles vaccine emphasize the need for a single shot anti-measles vaccine which is efficacious by mucosal route of administration and functional in the presence of anti-measles neutralizing antibodies. To achieve these goals, we constructed two recombinant human adenoviruses (collectively designated Ad-F/H) carrying genes for measles virus (MV) fusion (F) and haemagglutinin (H) proteins. Single intranasal or intramuscular vaccination of mice and cotton rats with Ad-F/H elicited high MV-specific serum neutralizing-antibody titers. Furthermore, bronchoalveolar lavage samples from mice vaccinated intranasally with Ad-F/H showed a 100-fold increase in MV-specific IgA titers compared with intramuscularly vaccinated mice. Moreover, Ad-F/H vaccine administered intranasally, but not intramuscularly, completely protected challenged cotton rats from MV replication in the lungs.
Lobanova
Liubov M
LM
Vaccine and Infectious Disease Organization, University of Saskatchewan, 120 Veterinary Road, Saskatoon, SK, S7N 5E3 Canada.
Baig
Tayyba T
TT
Tikoo
Suresh K
SK
Zakhartchouk
Alexander N
AN
D000256:Adenoviridae / Q000235:genetics*; D000281:Administration, Intranasal; D058956:Administration, Mucosal; D000818:Animals; D057134:Antibodies, Neutralizing / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology; D002522:Chlorocebus aethiops; D005260:Female; D005822:Genetic Vectors; D057809:HEK293 Cells; D006389:Hemagglutinins, Viral / Q000276:immunology; D006801:Humans; D018928:Immunity, Mucosal; D007070:Immunoglobulin A / Q000276:immunology; D007273:Injections, Intramuscular; D008168:Lung / Q000276:immunology / Q000382:microbiology; D008457:Measles / Q000276:immunology / Q000517:prevention & control*; D008458:Measles Vaccine / Q000235:genetics / Q000276:immunology*; D008459:Measles virus / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D009500:Neutralization Tests; D051381:Rats; D014709:Vero Cells; D014760:Viral Fusion Proteins / Q000276:immunology
D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D006389:Hemagglutinins, Viral; D007070:Immunoglobulin A; D008458:Measles Vaccine; D014760:Viral Fusion Proteins; C032397:hemagglutinin protein G, measles virus