Delivery of TFPI-2 using ultrasound with a microbubble agent (SonoVue) inhibits intimal hyperplasia after balloon injury in a rabbit carotid artery model.
Here we report a new, simple and efficient method by using ultrasound and a microbubble agent (SonoVue) for delivering a gene to balloon-injured carotid arteries for restenosis prophylaxis. The tissue factor pathway inhibitor-2 (TFPI-2) has been shown to inhibit the postinjury intimae hyperplasia in atherosclerotic vessels. New Zealand white rabbits were divided into 4 groups with 14 in each, a treatment control for balloon injury, a gene vehicle control, a gene delivery of TFPI-2 without using ultrasound and a gene delivery of TFPI-2 using ultrasound. After four weeks, the injured artery neointimal proliferation was significantly lower in the TFPI-2 group with ultrasound than the control groups (p < 0.01) according to the measurement of the mean luminal diameters by B-mode ultrasonography. The ratio of intimal/media area and the stenosis rate in the gene delivery facilitated by ultrasound were significantly lower than those of the nonultrasound gene delivering method (p < 0.01).
Zhou
Jie
J
Department of Biochemistry and Molecular Biology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Wang
Yuxue
Y
Xiong
Yufang
Y
Wang
Hongxing
H
Feng
Youmei
Y
Chen
Juan
J
D000704:Analysis of Variance; D000800:Angioplasty, Balloon; D000818:Animals; D001161:Arteriosclerosis / Q000628:therapy*; D003287:Contrast Media / Q000494:pharmacology; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods*; D006023:Glycoproteins / Q000378:metabolism*; D006965:Hyperplasia; D007091:Image Processing, Computer-Assisted; D007124:Immunoenzyme Techniques; D010743:Phospholipids / Q000494:pharmacology*; D010957:Plasmids; D011817:Rabbits; D013459:Sulfur Hexafluoride / Q000494:pharmacology*; D017539:Tunica Intima / Q000293:injuries*; D014465:Ultrasonics
D003287:Contrast Media; D006023:Glycoproteins; D010743:Phospholipids; C420843:contrast agent BR1; C021240:tissue-factor-pathway inhibitor 2; D013459:Sulfur Hexafluoride