title: Monolayer behavior of binary systems of betulinic acid and cardiolipin: thermodynamic analyses of Langmuir monolayers and AFM study of Langmuir-Blodgett monolayers.
abstract: Betulinic acid (BA, a natural pentacyclic triterpene) can induce mitochondrial membrane damage and trigger the mitochondrial pathway of apoptosis in tumor cells. The monolayer behavior of binary systems of BA and cardiolipin (CL, a unique phospholipid found only in mitochondria membrane in animals) was studied by surface pressure-area (π-A) measurements and analyses and Atomic force microscopy (AFM) observation. The miscibility analysis presents that in mixed monolayers BA takes both tilted and nearly perpendicular orientations at surface pressure below 30 mN/m but only nearly perpendicular orientation at 30 mN/m. The thermodynamic stability analysis indicates that phase separation and repulsion occur in mixed BA/CL monolayers. The compressibility analysis shows that at 30 mN/m, 20% addition of BA does markedly translate the liquid-condensed CL monolayer to mixed BA/CL monolayer with the coexistence of liquid-condensed and liquid-expanded phases. The AFM images of supported monolayers give direct evidence of the conclusions obtained from the analyses of π-A isotherms. These results confirm that at high surface pressure near to real biologic situations, BA orients nearly perpendicularly with hydroxyl group toward water, causes phase separation and changes the permeability of CL film, which correlates with the mitochondrial membrane damage induced by BA.
authors.affiliation: College of Physics and Information Technology, Shaanxi Normal University, Chang-an Street No. 199, Xi'an 710062, China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Chen
authors.forename: Runguang
authors.initials: R
authors.lastname: Sun
authors.forename: Bo
authors.initials: B
authors.lastname: Wang
mesh_terms: D000818:Animals; D002308:Cardiolipins / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D053978:Pentacyclic Triterpenes; D013499:Surface Properties; D013816:Thermodynamics; D014315:Triterpenes / Q000737:chemistry*; D000094062:Betulinic Acid
chemical_list: D002308:Cardiolipins; D053978:Pentacyclic Triterpenes; D014315:Triterpenes; D000094062:Betulinic Acid