Direct hapten-linked multiplexed immunoassays on polycarbonate surface.
Direct hapten-linked multiplexed immunoassay is developed on the polycarbonate surface of standard Digital Versatile Discs (DVDs) for six compounds of environmental concern, as proof of concept. Carboxylated haptens are directly linked to the aminated polycarbonate surface through carbodiimide/succinimide coupling. The modified DVD surface maintained its physical and optical properties. Multiplexed assay reached detection limits down to 0.1 μg/L for chlorpyrifos, 2,4,5-trichlorophenoxypropionic acid, sulfathiazole and sulfasalazine and down to 1.0 μg/L for fenthion and malathion. This approach presents advantages such as the improvement in sensitivity in comparison to protein-hapten conjugate format for all the studied analytes and the absence of cross-interference effects, allowing high throughput multianalysis on the same surface. Also, a comparison of the performance of two sensing strategies indicated that DVD disc and drive approach turned out in a simpler mode, the assays being more reproducible and with higher signal to noise ratios.
Tamarit-López
Jesús
J
Departamento de Química, Instituto de Reconocimiento Molecular y Desarrollo Tecnológico, Universidad Politécnica de Valencia, Camino de Vera s/n, 46071 Valencia, Spain.
Morais
Sergi
S
Puchades
Rosa
R
Maquieira
Ángel
Á
D016250:Compact Disks; D004784:Environmental Monitoring / Q000295:instrumentation*; D004785:Environmental Pollutants / Q000032:analysis*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006241:Haptens / Q000737:chemistry* / Q000276:immunology; D007118:Immunoassay / Q000295:instrumentation*; D011076:Polycarboxylate Cement / Q000737:chemistry*; D013499:Surface Properties
D004785:Environmental Pollutants; D006241:Haptens; D011076:Polycarboxylate Cement; C005506:polycarbonate