Ribosomal DNA, tri- and bi-partite pericentromeres in the permanent translocation heterozygote Rhoeo spathacea.
High- and low-stringency FISH and base-specific fluorescence were performed on the permanent translocation heterozygote Rhoeo spathacea (2n = 12). Our results indicate that 45S rDNA arrays, rDNA-related sequences and other GC-rich DNA fraction(s) are located within the pericentromeric regions of all twelve chromosomes, usually colocalizing with the chromomycin A(3)-positive bands. Homogenization of the pericentromeric regions appears to result from the concerted spread of GC-rich sequences, with differential amplification likely. We found new 5S rDNA patterns, which suggest a variability in the breakpoints and in the consequent chromosome reorganizations. It was found that the large 5S rDNA locus residing on each of the 8E and 9E arms consisted of two smaller loci. On each of the two chromosome arms 3b and 4b, in addition to the major subtelomeric 5S rDNA locus, a new minor locus was found interstitially about 40% along the arm length. The arrangement of cytotogenetic landmarks and chromosome arm measurements are discussed with regard to genome repatterning in Rhoeo.
Golczyk
Hieronim
H
Department of Plant Cytology and Embryology, Institute of Botany, Jagiellonian University, Kraków, Poland. h.golczyk@wp.pl
Hasterok
Robert
R
Szklarczyk
Marek
M
D002503:Centromere / Q000235:genetics*; D032461:Chromosomes, Plant; D018744:DNA, Plant / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D034823:Tradescantia / Q000235:genetics*; D014178:Translocation, Genetic
D018744:DNA, Plant; D004275:DNA, Ribosomal