title: Gene expression profiling of myelodysplastic CD34+ hematopoietic stem cells treated in vitro with decitabine.
abstract: Abnormal gene promoter methylation contributes to deregulate gene expression of hematopoietic progenitors in myelodysplastic syndromes (MDS). We analyzed the gene expression profile of myelodysplastic and normal CD34+ hematopoietic stem cells (HSCs) treated in vitro with decitabine. We identified a list of candidate tumor suppressor genes, expressed at low levels in MDS HSCs and induced by hypomethylating treatment only in MDS, but not in normal HSCs. Real-time RT-PCR confirmed reduced CD9 expression in MDS CD34+ and bone marrow mononuclear cells, compared to normal controls. CD9 was specifically up-regulated by decitabine treatment in myelodysplastic CD34+ cells.
authors.affiliation: Institute of Hematology, Università Cattolica del Sacro Cuore, Rome, Italy.
authors.forename: Manuela
authors.initials: M
authors.lastname: Giachelia
authors.forename: Francesco
authors.initials: F
authors.lastname: D'Alò
authors.forename: Emiliano
authors.initials: E
authors.lastname: Fabiani
authors.forename: Nathalie
authors.initials: N
authors.lastname: Saulnier
authors.forename: Annalisa
authors.initials: A
authors.lastname: Di Ruscio
authors.forename: Francesco
authors.initials: F
authors.lastname: Guidi
authors.forename: Stefan
authors.initials: S
authors.lastname: Hohaus
authors.forename: Maria Teresa
authors.initials: MT
authors.lastname: Voso
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Leone
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D018952:Antigens, CD34 / Q000097:blood; D000964:Antimetabolites, Antineoplastic / Q000494:pharmacology; D001374:Azacitidine / Q000031:analogs & derivatives* / Q000494:pharmacology; D016000:Cluster Analysis; D019175:DNA Methylation; D000077209:Decitabine; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D020869:Gene Expression Profiling; D006412:Hematopoietic Stem Cells / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D019012:Integrin beta1 / Q000235:genetics; D008297:Male; D008875:Middle Aged; D009190:Myelodysplastic Syndromes / Q000097:blood / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D011401:Promoter Regions, Genetic / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055815:Young Adult
chemical_list: D018952:Antigens, CD34; D000964:Antimetabolites, Antineoplastic; D019012:Integrin beta1; D000077209:Decitabine; D001374:Azacitidine