Atomic force microscopy study of DNA conformation in the presence of drugs.
Binding of ligands to DNA gives rise to several relevant biological and biomedical effects. Here, through the use of atomic force microscopy (AFM), we studied the consequences of drug binding on the morphology of single DNA molecules. In particular, we quantitatively analyzed the effects of three different DNA-binding molecules (doxorubicin, ethidium bromide, and netropsin) that exert various pharmacologic and therapeutic effects. The results of this study show the consequences of intercalation and groove molecular binding on DNA conformation. These single-molecule measurements demonstrate morphological features that reflect the specific modes of drug-DNA interaction. This experimental approach may have implications in the design of therapeutically effective agents.
Cassina
Valeria
V
Department of Experimental Medicine, University of Milano-Bicocca, Via Cadore 48, 20052, Monza, Milan, Italy. valeria.cassina@unimib.it
Seruggia
Davide
D
Beretta
Giovanni Luca
GL
Salerno
Domenico
D
Brogioli
Doriano
D
Manzini
Stefano
S
Zunino
Franco
F
Mantegazza
Francesco
F
D004247:DNA / Q000737:chemistry* / Q000187:drug effects / Q000648:ultrastructure; D004317:Doxorubicin / Q000737:chemistry* / Q000494:pharmacology; D004996:Ethidium / Q000737:chemistry* / Q000494:pharmacology; D007364:Intercalating Agents / Q000737:chemistry / Q000494:pharmacology; D008024:Ligands; D018625:Microscopy, Atomic Force / Q000379:methods; D009429:Netropsin / Q000737:chemistry* / Q000494:pharmacology; D009690:Nucleic Acid Conformation / Q000187:drug effects
D007364:Intercalating Agents; D008024:Ligands; D009429:Netropsin; D004317:Doxorubicin; D004247:DNA; D004996:Ethidium