title: Single-particle electron microscopy of animal fatty acid synthase describing macromolecular rearrangements that enable catalysis.
abstract: We have used macromolecular electron microscopy (EM) to characterize the conformational flexibility of the animal fatty acid synthase (FAS). Here we describe in detail methods employed for image collection and analysis. We also provide an account of how EM results were interpreted by considering a high-resolution static FAS X-ray structure and functional data to arrive at a molecular understanding of the way in which conformational pliability enables fatty acid synthesis.
authors.affiliation: Department of Cell Biology, The Scripps Research Institute, La Jolla, California, USA.
authors.forename: Edward J
authors.initials: EJ
authors.lastname: Brignole
authors.forename: Francisco
authors.initials: F
authors.lastname: Asturias
mesh_terms: D000818:Animals; D002384:Catalysis; D020134:Catalytic Domain; D020285:Cryoelectron Microscopy / Q000379:methods; D018360:Crystallography, X-Ray; D064429:Fatty Acid Synthases / Q000737:chemistry / Q000378:metabolism*; D008854:Microscopy, Electron; D011487:Protein Conformation
chemical_list: D064429:Fatty Acid Synthases