title: Investigating the structural biofunctionality of antibodies conjugated to magnetic nanoparticles.
abstract: We present the synthesis of trastuzumab-functionalized pegylated iron oxide nanoparticles and provide an FTIR-based approach to gain a direct evidence of the actual conservation of the native structure of conjugated antibody. Their target-selectivity to specific cancer cell receptors has been also assessed.
authors.affiliation: Dipartimento di Biotecnologie e Bioscienze, Università di Milano-Bicocca, P.za della Scienza 2, 20126, Milano, Italy.
authors.forename: Emanuela
authors.initials: E
authors.lastname: Occhipinti
authors.forename: Paolo
authors.initials: P
authors.lastname: Verderio
authors.forename: Antonino
authors.initials: A
authors.lastname: Natalello
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Galbiati
authors.forename: Miriam
authors.initials: M
authors.lastname: Colombo
authors.forename: Serena
authors.initials: S
authors.lastname: Mazzucchelli
authors.forename: Agnese
authors.initials: A
authors.lastname: Salvadè
authors.forename: Paolo
authors.initials: P
authors.lastname: Tortora
authors.forename: Silvia Maria
authors.initials: SM
authors.lastname: Doglia
authors.forename: Davide
authors.initials: D
authors.lastname: Prosperi
mesh_terms: D000906:Antibodies / Q000737:chemistry*; D000911:Antibodies, Monoclonal / Q000737:chemistry; D061067:Antibodies, Monoclonal, Humanized; D001224:Aspartic Acid / Q000031:analogs & derivatives / Q000737:chemistry; D045744:Cell Line, Tumor; D005290:Ferric Compounds / Q000737:chemistry; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D006801:Humans; D018796:Immunoconjugates / Q000737:chemistry; D047468:Immunoprecipitation; D008280:Magnetics; D053768:Metal Nanoparticles / Q000737:chemistry*; D018613:Microscopy, Confocal; D009025:Morpholines / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D000068878:Trastuzumab
chemical_list: D000906:Antibodies; D000911:Antibodies, Monoclonal; D061067:Antibodies, Monoclonal, Humanized; D005290:Ferric Compounds; D018796:Immunoconjugates; D009025:Morpholines; C499728:poly (3-morpholinopropyl) aspartamide; C000499:ferric oxide; D001224:Aspartic Acid; D016650:Fluorescein-5-isothiocyanate; D000068878:Trastuzumab