title: Isolation, pure culture and characterization of Serratia symbiotica sp. nov., the R-type of secondary endosymbiont of the black bean aphid Aphis fabae.
abstract: An intracellular symbiotic bacterium was isolated from the flora of a natural clone of the black bean aphid Aphis fabae. The strain was able to grow freely in aerobic conditions on a rich medium containing 1 % of each of the following substrates: glucose, yeast extract and casein peptone. Pure culture was achieved through the use of solid-phase culture on the same medium and the strain was designated CWBI-2.3(T). 16S rRNA gene sequence analysis revealed that strain CWBI-2.3(T) was a member of the class Gammaproteobacteria, having high sequence similarity (>99 %) with 'Candidatus Serratia symbiotica', the R-type of secondary endosymbiont that is found in several aphid species. As strain CWBI-2.3(T) ( = LMG 25624(T) = DSM 23270(T)) was the first R-type symbiont to be isolated and characterized, it was designated as the type strain of Serratia symbiotica sp. nov.
authors.affiliation: CWBI, University of Liege B40, 4000 Liege, Belgium.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Sabri
authors.affiliation: Department of Functional and Evolutionary Entomology, Gembloux Agro-Bio Tech, 5030 Gembloux, Belgium.
authors.forename: Pascal
authors.initials: P
authors.lastname: Leroy
authors.affiliation: Department of Functional and Evolutionary Entomology, Gembloux Agro-Bio Tech, 5030 Gembloux, Belgium.
authors.forename: Eric
authors.initials: E
authors.lastname: Haubruge
authors.affiliation: Unité d'Ecologie et de Biogéographie, 1348 Louvain-la-Neuve, Belgium.
authors.forename: Thierry
authors.initials: T
authors.lastname: Hance
authors.affiliation: Unité d'Ecologie et de Biogéographie, 1348 Louvain-la-Neuve, Belgium.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Frère
authors.affiliation: CWBI, University of Liege B40, 4000 Liege, Belgium.
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Destain
authors.affiliation: CWBI, University of Liege B40, 4000 Liege, Belgium.
authors.forename: Philippe
authors.initials: P
authors.lastname: Thonart
mesh_terms: D000332:Aerobiosis; D000818:Animals; D001042:Aphids / Q000382:microbiology*; D015373:Bacterial Typing Techniques; D016000:Cluster Analysis; D003470:Culture Media / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012705:Serratia / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D013559:Symbiosis
chemical_list: D003470:Culture Media; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S