Functional connectivity and selective odor responses of excitatory local interneurons in Drosophila antennal lobe.
Local interneurons in the Drosophila antennal lobe are thought to play important roles in shaping odor responses. However, the physiological properties of excitatory local interneurons (eLNs) and their connectivity in the antennal lobe remain unclear. We first characterized the firing patterns of krasavietz-Gal4-labeled eLNs (krasavietz eLNs) in response to depolarizing currents. Paired recordings of krasavietz eLNs and PNs showed reciprocal excitatory connections mediated by dendrodendritic cholinergic synapses and gap junctions. Reciprocal connections were also found between two krasavietz eLNs but were rare between krasavietz eLNs and inhibitory LNs. Analysis of response onset latencies showed that krasavietz eLNs received monosynaptic inputs from ORNs. Furthermore, each eLN responded with distinct patterns to different odors, and each odor elicited distinct responses in different eLNs, with specific temporal patterns of spiking, indicating that eLNs serve specific coding functions in addition to global excitation in Drosophila olfactory processing.
Huang
Ju
J
Institute of Neuroscience, State Key Laboratory of Neuroscience, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
Zhang
Wei
W
Qiao
Wenhui
W
Hu
Aiqun
A
Wang
Zuoren
Z
D000200:Action Potentials / Q000187:drug effects / Q000235:genetics; D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D030801:Animals, Genetically Modified; D002038:Bungarotoxins / Q000494:pharmacology; D018680:Cholinergic Antagonists / Q000494:pharmacology; D004330:Drosophila; D029721:Drosophila Proteins / Q000235:genetics; D004558:Electric Stimulation / Q000379:methods; D040061:Eukaryotic Initiation Factor-5 / Q000235:genetics; D005260:Female; D018756:GABA Antagonists / Q000494:pharmacology; D017629:Gap Junctions / Q000187:drug effects / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects; D049452:Green Fluorescent Proteins / Q000235:genetics; D053444:Inhibitory Postsynaptic Potentials / Q000187:drug effects / Q000235:genetics; D007395:Interneurons / Q000187:drug effects / Q000502:physiology*; D008239:Lysine / Q000031:analogs & derivatives / Q000378:metabolism; D008464:Mecamylamine / Q000494:pharmacology; D009415:Nerve Net / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D018733:Nicotinic Antagonists / Q000494:pharmacology; D009812:Odorants; D009833:Olfactory Pathways / Q000502:physiology; D009943:Organophosphorus Compounds / Q000494:pharmacology; D018408:Patch-Clamp Techniques; D010852:Picrotoxin / Q000494:pharmacology; D018048:Receptors, Purinergic P2 / Q000235:genetics / Q000378:metabolism; D058476:Receptors, Purinergic P2X2; D012679:Sense Organs / Q000166:cytology*; D012903:Smell / Q000502:physiology*
D000255:Adenosine Triphosphate; D002038:Bungarotoxins; D018680:Cholinergic Antagonists; D029721:Drosophila Proteins; D040061:Eukaryotic Initiation Factor-5; D018756:GABA Antagonists; D049452:Green Fluorescent Proteins; D008239:Lysine; D008464:Mecamylamine; D018733:Nicotinic Antagonists; D009943:Organophosphorus Compounds; D010852:Picrotoxin; D018048:Receptors, Purinergic P2; D058476:Receptors, Purinergic P2X2; C520428:Krasavietz protein, Drosophila; C107353:CGP 54626; C013411:biocytin