title: Conservation, duplication, and loss of the Tor signaling pathway in the fungal kingdom.
abstract: BACKGROUND
  The nutrient-sensing Tor pathway governs cell growth and is conserved in nearly all eukaryotic organisms from unicellular yeasts to multicellular organisms, including humans. Tor is the target of the immunosuppressive drug rapamycin, which in complex with the prolyl isomerase FKBP12 inhibits Tor functions. Rapamycin is a gold standard drug for organ transplant recipients that was approved by the FDA in 1999 and is finding additional clinical indications as a chemotherapeutic and antiproliferative agent. Capitalizing on the plethora of recently sequenced genomes we have conducted comparative genomic studies to annotate the Tor pathway throughout the fungal kingdom and related unicellular opisthokonts, including Monosiga brevicollis, Salpingoeca rosetta, and Capsaspora owczarzaki.
  
  
  RESULTS
  Interestingly, the Tor signaling cascade is absent in three microsporidian species with available genome sequences, the only known instance of a eukaryotic group lacking this conserved pathway. The microsporidia are obligate intracellular pathogens with highly reduced genomes, and we hypothesize that they lost the Tor pathway as they adapted and streamlined their genomes for intracellular growth in a nutrient-rich environment. Two TOR paralogs are present in several fungal species as a result of either a whole genome duplication or independent gene/segmental duplication events. One such event was identified in the amphibian pathogen Batrachochytrium dendrobatidis, a chytrid responsible for worldwide global amphibian declines and extinctions.
  
  
  CONCLUSIONS
  The repeated independent duplications of the TOR gene in the fungal kingdom might reflect selective pressure acting upon this kinase that populates two proteinaceous complexes with different cellular roles. These comparative genomic analyses illustrate the evolutionary trajectory of a central nutrient-sensing cascade that enables diverse eukaryotic organisms to respond to their natural environments.
authors.affiliation: Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC 27710, USA.
authors.forename: Cecelia A
authors.initials: CA
authors.lastname: Shertz
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Bastidas
authors.forename: Wenjun
authors.initials: W
authors.lastname: Li
authors.forename: Joseph
authors.initials: J
authors.lastname: Heitman
authors.forename: Maria E
authors.initials: ME
authors.lastname: Cardenas
mesh_terms: D000595:Amino Acid Sequence; D017124:Conserved Sequence / Q000235:genetics*; D019143:Evolution, Molecular; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D005658:Fungi / Q000201:enzymology* / Q000235:genetics*; D020440:Gene Duplication / Q000235:genetics*; D016681:Genome, Fungal / Q000235:genetics; D016814:Microsporidia / Q000201:enzymology / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics; D012568:Schizosaccharomyces / Q000201:enzymology / Q000235:genetics; D015398:Signal Transduction / Q000235:genetics*; D026801:Synteny
chemical_list: D005656:Fungal Proteins