[Screening of candidate proteins interacting with Foxp3δ2 by yeast two-hybrid system].
OBJECTIVE
To screen Foxp3Delta2-interacting proteins by yeast two-hybria.


METHODS
pGBKT7-Foxp3Delta2 bait plasmid was constructed. Its toxicity and transcriptional activation in yeast cell AH109 was tested by observation of phenotypes and color.


RESULTS
The bait plasmid pGBKT7-Foxp3Delta2 was constructed successfully and there was no self-activation or toxicity in AH109. Forty positive clones were obtained. After sequence comparison and analysis, nine candidate proteins were finally selected for further confirmation.


CONCLUSIONS
Yeast two hybrid system could be used for screening Foxp3Delta2-interacting proteins. These results provide essential clues for further investigation of Foxp3Delta2 function.
Xu
Yu-jin
YJ
Biotechnology Center, Pharmacy Department, Fourth Military Medical University, Xi'an, China.
Jiang
Chang-li
CL
Zhang
Cun
C
Qin
Xin
X
Li
Meng
M
Hao
Qiang
Q
Li
Wei-na
WN
Zhang
Wei
W
Zhang
Ying-qi
YQ
D051858:Forkhead Transcription Factors / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008297:Male; D011485:Protein Binding; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D020798:Two-Hybrid System Techniques
C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors