Comparison of the chromatographic properties of IgG1 and IgG2 antibody subclasses.
All therapeutic antibodies that are currently approved or under development are IgGs, with IgG1 and IgG2 being the most prevalent subclasses. The present study investigates the effect of antibody subclass on its chromatographic properties. To this end, two sets of antibodies with identical variable domains and differing subclasses (IgG1 and IgG2) were compared by using purification methods that are most commonly used in industrial antibody manufacturing processes. Each antibody was examined using Protein A chromatography, cation-exchange chromatography, hydrophobic interaction chromatography and salt-induced precipitation studies. The results of these experiments show that the variable region of an antibody has a greater influence on its chromatographic behaviour than antibody subclass.
Ghose
Sanchayita
S
Purification Process Development, Amgen, Seattle, WA 98119, USA. sanchayita.ghose@bms.com
Ladwig
Justin
J
Allen
Martin
M
D000818:Animals; D016466:CHO Cells; D011232:Chemical Precipitation; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D006224:Cricetinae; D003412:Cricetulus; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D007074:Immunoglobulin G / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D012492:Salts / Q000737:chemistry; D013205:Staphylococcal Protein A / Q000378:metabolism
D007074:Immunoglobulin G; D012492:Salts; D013205:Staphylococcal Protein A