Stable producer cell lines for adeno-associated virus (AAV) assembly.
Stable producer cell lines containing both the rep and cap genes and recombinant adeno-associated virus (rAAV) vectors can be infected with a helper virus to provide reliable and efficient production of rAAV stocks. However, the development of these cell lines is time-consuming. The procedure described here is therefore recommended only for studies requiring the production of high amounts of rAAV, such as preclinical studies performed in large animals.
Chadeuf
Gilliane
G
Salvetti
Anna
A
D000818:Animals; D002460:Cell Line; D000229:Dependovirus / Q000235:genetics / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D011994:Recombinant Proteins / Q000235:genetics; D014779:Virus Replication
D011994:Recombinant Proteins