SOLiD sequencing of four Vibrio vulnificus genomes enables comparative genomic analysis and identification of candidate clade-specific virulence genes.
BACKGROUND
Vibrio vulnificus is the leading cause of reported death from consumption of seafood in the United States. Despite several decades of research on molecular pathogenesis, much remains to be learned about the mechanisms of virulence of this opportunistic bacterial pathogen. The two complete and annotated genomic DNA sequences of V. vulnificus belong to strains of clade 2, which is the predominant clade among clinical strains. Clade 2 strains generally possess higher virulence potential in animal models of disease compared with clade 1, which predominates among environmental strains. SOLiD sequencing of four V. vulnificus strains representing different clades (1 and 2) and biotypes (1 and 2) was used for comparative genomic analysis.


RESULTS
Greater than 4,100,000 bases were sequenced of each strain, yielding approximately 100-fold coverage for each of the four genomes. Although the read lengths of SOLiD genomic sequencing were only 35 nt, we were able to make significant conclusions about the unique and shared sequences among the genomes, including identification of single nucleotide polymorphisms. Comparative analysis of the newly sequenced genomes to the existing reference genomes enabled the identification of 3,459 core V. vulnificus genes shared among all six strains and 80 clade 2-specific genes. We identified 523,161 SNPs among the six genomes.


CONCLUSIONS
We were able to glean much information about the genomic content of each strain using next generation sequencing. Flp pili, GGDEF proteins, and genomic island XII were identified as possible virulence factors because of their presence in virulent sequenced strains. Genomic comparisons also point toward the involvement of sialic acid catabolism in pathogenesis.
Gulig
Paul A
PA
Department of Molecular Genetics and Microbiology, University of Florida, Gainesville, Florida, USA. gulig@ufl.edu
de Crécy-Lagard
Valérie
V
Wright
Anita C
AC
Walts
Brandon
B
Telonis-Scott
Marina
M
McIntyre
Lauren M
LM
D000818:Animals; D001483:Base Sequence; D005798:Genes, Bacterial / Q000235:genetics*; D023281:Genomics / Q000379:methods*; D005838:Genotype; D051379:Mice; D016366:Open Reading Frames / Q000235:genetics; D010641:Phenotype; D010802:Phylogeny; D010957:Plasmids / Q000235:genetics; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D012015:Reference Standards; D017422:Sequence Analysis, DNA / Q000379:methods*; D041261:Vibrio vulnificus / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity*; D014774:Virulence / Q000235:genetics