Mesenchymal stem cells isolated from human uterine cervix cancer tissues.
In the present study, MSCs (mesenchymal stem cells) were successfully isolated and identified from hUCC (human uterine cervix cancer) tissues. The morphological appearance, immunophenotype, growth curve, cell cycle, cytogenetic features and differentiation potential of these cells were investigated. Results showed that cells isolated from the uterine cervix cancer tissues displayed fibroblast-like morphology and grew into colonies. Immunophenotyping by flow cytometry revealed that the isolated cells were positive for CD13, CD29, CD44, CD105 and HLA-I, while negative for CD10, CD14, CD31, CD34, CD38 and HLA-DR. The cells kept a normal karyotype by chromosome analysis. At the third passage, the percentages of cells in G0-/G1-, 2-/M- and S-phase were 84.94, 8.36 and 6.71%, respectively. Under appropriate induction conditions, these cells can differentiate into osteogenic, adipogenic cells and hepatocytes. Taken together, MSCs were confirmed to exist in hUCC tissues, which may provide a new target for clinical cancer therapy.
Sun
Xiaochun
X
School of Medical Science and Laboratory Medicine, Key Institute of Clinical Laboratory Science, Jiangsu University, Zhenjiang Key Institute of Clinical Laboratory Medicine, 301 Xuefu Road, Zhenjiang City, Jiangsu Province 212013, Peoples Republic of China.
Cai
Hua
H
Qian
Hui
H
Zhu
Wei
W
Yan
Yongmin
Y
Xu
Huaxi
H
Xu
Wenrong
W
D015703:Antigens, CD / Q000032:analysis; D002454:Cell Differentiation; D002455:Cell Division; D002478:Cells, Cultured; D002584:Cervix Uteri / Q000473:pathology*; D005260:Female; D006801:Humans; D016130:Immunophenotyping; D007399:Interphase; D007621:Karyotyping; D059630:Mesenchymal Stem Cells / Q000473:pathology*; D002583:Uterine Cervical Neoplasms / Q000473:pathology*
D015703:Antigens, CD