Identification of a red-emitting fluorescent ligand for in vitro visualization of human serotonin 5-HT(1A) receptors.
The 5-HT(1A) receptor subtype is the most thoroughly studied serotonin receptor subtype. We report here the design, synthesis and characterization of two new fluorescent ligands for the 5-HT(1A) receptor. The new 1-arylpiperazine-based red-emitting fluorescent compound 6 displayed good binding affinity at the 5-HT(1A) receptor (K(i)=35 nM) and was able to label specifically the human 5-HT(1A) receptor stably expressed in CHO cells visualized using confocal laser scanning microscopy.
Lacivita
Enza
E
Università degli Studi di Bari A. Moro, Dipartimento Farmaco-Chimico, via Orabona, 4, 70125 Bari, Italy.
Masotti
Anna Carmela
AC
Jafurulla
Md
M
Saxena
Roopali
R
Rangaraj
Nandini
N
Chattopadhyay
Amitabha
A
Colabufo
Nicola A
NA
Berardi
Francesco
F
Perrone
Roberto
R
Leopoldo
Marcello
M
D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D008024:Ligands; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D044282:Receptor, Serotonin, 5-HT1A / Q000737:chemistry*
D005456:Fluorescent Dyes; D008024:Ligands; D044282:Receptor, Serotonin, 5-HT1A