title: Purification of polar compounds from Radix isatidis using conventional C18 column coupled with polar-copolymerized C18 column.
abstract: Regarding hydrophilic interaction chromatography and normal phase liquid chromatography, RPLC is another choice used to separate polar compounds with the improvement of polar-modified C18 stationary phase. In this study, a method using conventional C18 column coupled with polar-copolymerized C18 column was successfully developed for the separation and purification of polar compounds from Radix isatidis, which is one of the most commonly used traditional Chinese medicines (TCMs). An XTerra MS C18 column was used to fractionate the extract of R. isatidis and a homemade polar-copolymerized C18 column was utilized for the final purification due to its good separation selectivity and high resolution for polar compounds. The established purification system demonstrated good orthogonality for the polar compounds. As a result, ten compounds were purified and three of them were identified as 3-methyl-5-vinyloxazolidin-2-one (compound A), 5-hydroxymethyl-2-furaldehyde (compound B) and 3-methylfuran-2-carboxylic acid (compound G) based on the MS, IR and extensive NMR data, respectively. It was demonstrated to be a feasible and powerful technique for the purification of polar compounds under RPLC mode and more chemical information of TCMs will be obtained to interpret the efficiency of TCMs.
authors.affiliation: Key Lab of Separation Science for Analytical Chemistry, Key Lab of Natural Medicine, Liaoning Province, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zeng
authors.forename: Zhimou
authors.initials: Z
authors.lastname: Guo
authors.forename: Yuansheng
authors.initials: Y
authors.lastname: Xiao
authors.forename: Chaoran
authors.initials: C
authors.lastname: Wang
authors.forename: Xiuli
authors.initials: X
authors.lastname: Zhang
authors.forename: Xinmiao
authors.initials: X
authors.lastname: Liang
mesh_terms: D002264:Carboxylic Acids / Q000302:isolation & purification*; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005662:Furaldehyde / Q000031:analogs & derivatives* / Q000302:isolation & purification; D005663:Furans / Q000302:isolation & purification; D008516:Medicine, Chinese Traditional; D015394:Molecular Structure; D018517:Plant Roots / Q000737:chemistry; D014753:Vinyl Compounds / Q000302:isolation & purification*
chemical_list: D002264:Carboxylic Acids; D004365:Drugs, Chinese Herbal; D005663:Furans; D014753:Vinyl Compounds; C039851:3-methylfuran; C008046:5-hydroxymethylfurfural; D005662:Furaldehyde