Cytotoxicity of Vibrio cholerae on Madin Darby Bovine Kidney cells.
The cytotoxicity of cell-free culture filtrates of 31 isolates of Vibrio cholerae O1 and O139, 5 reference strains and 26 clinical isolates, was tested on Madin Darby Bovine Kidney (MDBK) cells and Vero cells. The 3-[4,5-dimethylthiazol-2-y]-2, 5-diphenyltetrazolium bromide (MTT) test was used to detect the effect of the filtrates on the proliferation and viability of cultured cell populations. The filtrates were prepared from serial ten-fold dilutions of inoculated AKI and APW broth media with and without the addition of polymyxin B. The APW culture filtrates of both V. cholerae O1 and O139 with and without added polymyxin B showed greater toxicity to MDBK cells as compared to AKI filtrates. The cytotoxicity of AKI-grown V. cholerae O139 to MDBK cells was greater than that of V. cholerae O1 grown in the same medium. The cytotoxicity of APW filtrates on Vero cells was low and only noted when polymyxin was added to the medium.
Haque
Quazi Manjurul
QM
Department of Basic Medical Sciences, International Islamic University Malaysia, Jalan Istana, Pahang Darul Makmur, Malaysia.
Mohamad
Najmiyatul Fadilah
NF
Helaluddin
A B M
AB
Saeed
Mohammad
M
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology*; D002417:Cattle; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002522:Chlorocebus aethiops; D004396:Coloring Agents; D003470:Culture Media / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay; D007668:Kidney / Q000187:drug effects*; D011112:Polymyxin B / Q000494:pharmacology; D013778:Tetrazolium Salts; D013844:Thiazoles; D014709:Vero Cells; D014734:Vibrio cholerae / Q000737:chemistry* / Q000187:drug effects
D000900:Anti-Bacterial Agents; D000970:Antineoplastic Agents; D004396:Coloring Agents; D003470:Culture Media; D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue; D011112:Polymyxin B