Isothermal detection of RNA with restriction endonucleases.
Herein, we demonstrate how to detect nucleic acids that do not contain restriction endonuclease recognition sites with restriction endonucleases. We show that the topology of DNA probes used in this detection strategy remarkably affects the efficiency of RNA/DNA detection.
Yan
Lei
L
Department of Chemistry and Biochemistry, University of Maryland, College Park, MD 20742, USA.
Nakayama
Shizuka
S
Yitbarek
Saron
S
Greenfield
Isabel
I
Sintim
Herman O
HO
D015342:DNA Probes / Q000737:chemistry; D004262:DNA Restriction Enzymes / Q000737:chemistry* / Q000378:metabolism; D012313:RNA / Q000032:analysis*; D013696:Temperature
D015342:DNA Probes; D012313:RNA; D004262:DNA Restriction Enzymes