Benefits of blood group genotyping in multi-transfused patients from the south of Brazil.
We evaluated the usefulness of blood group genotyping as a supplement to hemagglutination to determine the red blood cell (RBC) antigen profile of polytransfused patients with hematological diseases and renal failure. Seventy-nine patients were selected. They all received more than three units of blood and eight (10%) had already clinical significant alloantibodies occurring alone or in combination against Rh, K, Fya, and Di antigens. DNA was prepared from blood samples and RHCE*E/e, KEL*01/KEL*02, FY*01/FY*02 and JK*01/JK*02 alleles were determined by using PCR-RFLP. RHD*/RHD*Ψ and RHCE*C/c were tested using multiplex PCR. Discrepancies for Rh, Kell, Duffy, and Kidd systems were found between the phenotype and genotype-derived phenotype in 16 of the 38 chronically transfused patients. The genotypes of these patients were confirmed by DNA array analysis (HEA Beadchip(™); Bioarray Solutions, Warren, NJ). Genotyping was very important for the determination of the true blood groups of the polytransfused patients, helped in the identification of suspected alloantibodies and in the selection of antigen-negative RBCs for transfusion.
Guelsin
Gláucia Andréia Soares
GA
Laboratório de Imunogenética, Departamento de Ciências Básicas da Saúde, Universidade Estadual de Maringá, Maringá, Brazil.
Sell
Ana Maria
AM
Castilho
Lilian
L
Masaki
Viviane Lika
VL
Melo
Fabiano Cavalcante
FC
Hashimoto
Margareth Naomi
MN
Higa
Tatiana Takahashi
TT
Hirle
Loide Souza
LS
Visentainer
Jeane Eliete Laguila
JE
D001789:Blood Group Antigens / Q000032:analysis / Q000235:genetics*; D001803:Blood Transfusion; D001938:Brazil; D016022:Case-Control Studies; D004247:DNA / Q000235:genetics*; D004375:Duffy Blood-Group System / Q000097:blood / Q000235:genetics*; D004912:Erythrocytes; D005838:Genotype; D006402:Hematologic Diseases / Q000097:blood / Q000235:genetics / Q000628:therapy; D006801:Humans; D007626:Kell Blood-Group System / Q000097:blood / Q000235:genetics*; D010641:Phenotype; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D011379:Prognosis; D051437:Renal Insufficiency / Q000097:blood / Q000235:genetics / Q000628:therapy; D012204:Rh-Hr Blood-Group System / Q000097:blood / Q000235:genetics*
D001789:Blood Group Antigens; D004375:Duffy Blood-Group System; D007626:Kell Blood-Group System; D012204:Rh-Hr Blood-Group System; D004247:DNA