"Isolation, identification, characterization and polymetallic concentrate leaching studies of tryptic soy- and peptone-resistant thermotolerant Acidithiobacillus ferrooxidans SRDSM2".
Acidithiobacillus ferrooxidans strain SRDSM2 was isolated from silica containing soil sample collected at a Rajpardi lignite mine. The strain responded to the addition of 0.5 g/L peptone and 1.0 g/L tryptone soya broth in the ferrous sulphate tryptone soya broth (ITSB) medium with 35.3% and 29.6% increase in iron oxidation rate (IOR), but decrease in the IOR at higher peptone or tryptone soya broth levels. The presence of 4 mM of zinc as zinc sulphate in the medium increased the IOR by 24.4%. Forty percent of the inoculated cells survived even after exposure at 80 °C for 120 min and showed 30% ferrous iron oxidation. The Vmax and Ks for iron oxidation by the isolate were 344.82 mg/L/h and 32.25 g/L respectively. The isolate was able to oxidized ferrous iron even in presence of 4.06 M ionic strength of medium and leached>85% copper and zinc from the polymetallic concentrate. Thus, this isolate can be used for bioextraction of metals from polymetallic concentrate.
Patel
Mitesh J
MJ
Department of Microbiology, School of Sciences, Gujarat University, Ahmedabad 380 009, Gujarat, India.
Tipre
Devayani R
DR
Dave
Shailesh R
SR
D042763:Acidithiobacillus / Q000187:drug effects / Q000302:isolation & purification*; D000143:Acids / Q000378:metabolism; D000222:Adaptation, Physiological / Q000187:drug effects*; D001673:Biodegradation, Environmental / Q000187:drug effects; D002364:Caseins / Q000494:pharmacology*; D003300:Copper / Q000494:pharmacology; D004785:Environmental Pollutants / Q000302:isolation & purification*; D005296:Ferrous Compounds / Q000378:metabolism; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D007194:India; D007501:Iron / Q000378:metabolism; D008670:Metals / Q000302:isolation & purification*; D008906:Mining; D010084:Oxidation-Reduction / Q000187:drug effects; D010461:Peptones / Q000494:pharmacology*; D011143:Polyvinyl Chloride; D011492:Protein Hydrolysates / Q000494:pharmacology*; D013696:Temperature; D015032:Zinc / Q000494:pharmacology
D000143:Acids; D002364:Caseins; D004785:Environmental Pollutants; D005296:Ferrous Compounds; D008670:Metals; D010461:Peptones; D011492:Protein Hydrolysates; C026375:trypticase-soy broth; C020748:ferrous sulfate; D003300:Copper; D011143:Polyvinyl Chloride; D007501:Iron; D015032:Zinc