Successful transfer of chemical-induced asthma by adoptive transfer of low amounts of lymphocytes in a mouse model.
BACKGROUND
We optimized an adoptive transfer protocol in our mouse model of TDI-induced asthma in order to investigate the mechanisms of this type of occupational asthma.


METHODS
On days 1 and 8, BALB/c mice were dermally sensitized with 0.3% TDI or vehicle (acetone/olive oil), and on day 15, they were sacrificed and a cell suspension was made from auricular lymph nodes. First, 0.1 x 10⁶, 0.5 x 10⁶, 1 x 10⁶ or 5 x 10⁶ cells were injected intravenously into naïve mice and three days later these mice received an oropharyngeal challenge with 0.01% TDI or vehicle. Second, mice were challenged with 0.01% TDI 1, 3, 5 or 7 days after transferring 0.5 x 10⁶ cells. The following endpoints were measured one day after challenge: methacholine reactivity; differential cell counts in bronchoalveolar lavage (BAL) and total serum IgE.


RESULTS
Naïve mice receiving 0.5 x 10⁶, 1 x 10⁶ or 5 x 10⁶ cells showed significant increases in airway reactivity one day after TDI challenge; BAL neutrophils were increased after transferring 0.5 x 10⁶ and 1 x 10⁶ cells. A TDI challenge 3 days after transferring 0.5 x 10⁶ cells gave a 3-fold increase in airway resistance and a pronounced airway inflammation, whereas challenging at other time points gave no differences.


CONCLUSIONS
We were able to passively sensitize naïve mice using lymph node cells from TDI-sensitized mice, resulting in an asthma-like response after an airway challenge. In comparison to other adoptive transfer protocols we used substantially lower number of cells to obtain the desired response.
De Vooght
Vanessa
V
Research Unit of Lung Toxicology, Katholieke Universiteit Leuven, Herestraat 49 bus 706, 3000 Leuven, Belgium. vanessa.devooght@med.kuleuven.be
Haenen
Steven
S
Verbeken
Erik
E
Nemery
Benoit
B
Hoet
Peter H M
PH
Vanoirbeek
Jeroen A J
JA
D019264:Adoptive Transfer / Q000379:methods*; D000818:Animals; D001249:Asthma / Q000276:immunology*; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology; D016085:Bronchoconstrictor Agents / Q000494:pharmacology; D004195:Disease Models, Animal; D007073:Immunoglobulin E / Q000097:blood; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D016210:Methacholine Chloride / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D009504:Neutrophils / Q000276:immunology; D013997:Time Factors; D014051:Toluene 2,4-Diisocyanate / Q000633:toxicity*
D016085:Bronchoconstrictor Agents; D016210:Methacholine Chloride; D014051:Toluene 2,4-Diisocyanate; D007073:Immunoglobulin E