Identification of a plant gene encoding glutamate/aspartate-prephenate aminotransferase: the last homeless enzyme of aromatic amino acids biosynthesis.
In all organisms synthesising phenylalanine and/or tyrosine via arogenate, a prephenate aminotransferase is required for the transamination of prephenate into arogenate. The identity of the gene encoding this enzyme in the organisms where this activity occurs is still unknown. Glutamate/aspartate-prephenate aminotransferase (PAT) is thus the last homeless enzyme in the aromatic amino acids pathway. We report on the purification, mass spectrometry identification and biochemical characterization of Arabidopsis thaliana prephenate aminotransferase. Our data revealed that this activity is housed by the prokaryotic-type plastidic aspartate aminotransferase (At2g22250). This represents the first identification of a gene encoding PAT.
Graindorge
Matthieu
M
Université Grenoble 1, Grenoble, France.
Giustini
Cécile
C
Jacomin
Anne Claire
AC
Kraut
Alexandra
A
Curien
Gilles
G
Matringe
Michel
M
D024322:Amino Acids, Aromatic / Q000378:metabolism*; D017360:Arabidopsis / Q000166:cytology / Q000201:enzymology / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D001219:Aspartate Aminotransferases / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D018698:Glutamic Acid / Q000378:metabolism; D007700:Kinetics; D013058:Mass Spectrometry; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism; D000637:Transaminases / Q000235:genetics / Q000378:metabolism*
D024322:Amino Acids, Aromatic; D029681:Arabidopsis Proteins; D011994:Recombinant Proteins; D018698:Glutamic Acid; D000637:Transaminases; C044954:prephenate aminotransferase; D001219:Aspartate Aminotransferases