title: [Construction and identification of shRNA expressing vectors targeting RhoA gene].
abstract: OBJECTIVE
  To construct and identify shRNA interference vector targeting RhoA gene.
  
  
  METHODS
  Two pairs of single stranded oligonucleotides encoding RhoA siRNA sequence were designed and synthesized. After annealing, its were inserted into vector pGPU6/GFP/Neo, constructed recombinant vectors and then were identified by restrictive digestion and DNA sequencing. LoVo cells were transfected with the recombinant DNA samples by the liposome complex method, and then fluorescence photographs were taken.
  
  
  RESULTS
  Enzyme digestion and DNA sequencing showed that the oligonucleotide fragments were correctly inserted into pGPU6/GFP/Neo vector.
  
  
  CONCLUSIONS
  The RhoA gene-targeted siRNA and its vector are successfully constructed.
authors.affiliation: Department of General Surgery, Third Xiangya Hospital, Center South University, Changsha, China. soskinghero@126.com
authors.forename: Kai-yan
authors.initials: KY
authors.lastname: Yang
authors.forename: Dao-jin
authors.initials: DJ
authors.lastname: Chen
authors.forename: Xiao-rong
authors.initials: XR
authors.lastname: Li
authors.forename: Yan-guang
authors.initials: YG
authors.lastname: Wen
mesh_terms: D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000235:genetics / Q000378:metabolism / Q000628:therapy; D018390:Gene Targeting / Q000295:instrumentation* / Q000379:methods; D005822:Genetic Vectors / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics* / Q000378:metabolism; D014162:Transfection; D020742:rhoA GTP-Binding Protein / Q000235:genetics* / Q000378:metabolism
chemical_list: D034741:RNA, Small Interfering; D020742:rhoA GTP-Binding Protein