title: Insulin-like growth factor-binding protein-2 and -3 in gingival crevicular fluid.
abstract: OBJECTIVE
  Insulin-like growth factor-binding proteins (IGFBPs) are crucial regulators of insulin-like growth factor (IGF). They enhance or inhibit IGF functions, but also exhibit IGF-independent effects. In a previous study, we detected, qualitatively, IGFBP-2 and -3 in gingival crevicular fluid using a cytokine antibody array. Here we extended these results using an ELISA to determine the concentrations of IGFBP-2 and -3 in gingival crevicular fluid. In addition, we explored whether the expression of IGFBP-2 and IGFBP-3 correlates with periodontal disease severity.
  
  
  METHODS
  Gingival crevicular fluid samples from 92 sites of 12 patients affected with periodontal disease and from 100 sites of 19 healthy volunteers, were collected, divided into two groups and analyzed by ELISA for IGFBP-2 and -3 expression. The potential correlation among probing depth, gingival index and the concentrations of IGFBP-2 and -3 was analyzed.
  
  
  RESULTS
  Positive correlations were observed between the concentration of IGFBP-2 and probing depth and gingival index, but not for IGFBP-3. The IGFBP-2 concentrations at bleeding on probing-positive sites and at sites with a probing depth of ≥ 4 mm were higher than at bleeding on probing-negative sites and at sites with a probing depth of ≤ 3 mm.
  
  
  CONCLUSIONS
  These results indicate that IGFBP-2 is a potential novel marker for periodontal disease progression. As IGFBP-2 modulates bone metabolism and cell migration, IGFBP-2 in the gingival crevicular fluid may reflect periodontal disease activity.
authors.affiliation: Department of Periodontology, Nihon University School of Dentistry, Tokyo, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Takenouchi
authors.forename: M
authors.initials: M
authors.lastname: Ohshima
authors.forename: Y
authors.initials: Y
authors.lastname: Yamaguchi
authors.forename: T
authors.initials: T
authors.lastname: Nishida
authors.forename: N
authors.initials: N
authors.lastname: Senda
authors.forename: M
authors.initials: M
authors.lastname: Idesawa
authors.forename: K
authors.initials: K
authors.lastname: Otsuka
authors.forename: K
authors.initials: K
authors.lastname: Ito
mesh_terms: D000328:Adult; D000368:Aged; D015415:Biomarkers; D016022:Case-Control Studies; D018450:Disease Progression; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015870:Gene Expression; D005883:Gingival Crevicular Fluid / Q000737:chemistry*; D006801:Humans; D018971:Insulin-Like Growth Factor Binding Protein 2 / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D018972:Insulin-Like Growth Factor Binding Protein 3 / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D008297:Male; D008875:Middle Aged; D010512:Periodontal Index; D010518:Periodontitis / Q000378:metabolism*; D055815:Young Adult
chemical_list: D015415:Biomarkers; D018971:Insulin-Like Growth Factor Binding Protein 2; D018972:Insulin-Like Growth Factor Binding Protein 3