title: Expression of active and inactive recombinant soluble trehalase using baculovirus-silkworm expression system and their glycan structures.
abstract: Silkworm soluble trehalase was expressed as a fusion protein with an N-terminal or C-terminal hexahistidine tag in a baculovirus-silkworm expression system and assayed for enzymatic activity. Only N-terminally tagged trehalase showed a high activity. This study is the first to report in vitro functional expression of recombinant insect soluble trehalase.
authors.affiliation: Laboratory of Biological Chemistry, Department of Applied Biological Chemistry, Faculty of Agriculture, Meijo University, Tempaku-ku, Nagoya 468-8502, Japan. ujita@meijo-u.ac.jp
authors.forename: Minoru
authors.initials: M
authors.lastname: Ujita
authors.forename: Mayumi
authors.initials: M
authors.lastname: Yamanaka
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Maeno
authors.forename: Koki
authors.initials: K
authors.lastname: Yoshida
authors.forename: Wakana
authors.initials: W
authors.lastname: Ohshio
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Ueno
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Banno
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Fujii
authors.forename: Hiroki
authors.initials: H
authors.lastname: Okumura
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D012831:Bombyx / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D003001:Cloning, Molecular; D015870:Gene Expression; D005822:Genetic Vectors; D006639:Histidine; D009842:Oligopeptides; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D014198:Trehalase / Q000096:biosynthesis* / Q000235:genetics
chemical_list: C471213:His-His-His-His-His-His; D009842:Oligopeptides; D011993:Recombinant Fusion Proteins; D006639:Histidine; D014198:Trehalase