title: Interference of Mycoplasma infection in a gene expression study: it was the environment and not the gene.
abstract: We show that short-term exposure to doxycycline, as used in tetracycline-inducible gene expression models, protects cells from stress-induced death in cultures infected with Mycoplasma arginini. Coinciding with the expected maximum level of gene activity, antimicrobial effects of tetracyclines might be mistaken for antiapoptotic properties of the expressed gene product.
authors.affiliation: Division of Medical and Chemical Laboratory Diagnosis, Department of Laboratory Medicine, Medical University of Vienna, Währinger Gürtel 18-20, A-1090 Vienna, Austria.
authors.forename: Gregor
authors.initials: G
authors.lastname: Hoermann
authors.forename: Joachim
authors.initials: J
authors.lastname: Spergser
authors.forename: Elisa
authors.initials: E
authors.lastname: Einwallner
authors.forename: Athanasios
authors.initials: A
authors.lastname: Makristathis
authors.forename: Andrea
authors.initials: A
authors.lastname: Perne
authors.forename: Birgit
authors.initials: B
authors.lastname: Willinger
authors.forename: Ilse
authors.initials: I
authors.lastname: Schwarzinger
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002460:Cell Line; D015870:Gene Expression; D051379:Mice; D009174:Mycoplasma / Q000187:drug effects* / Q000472:pathogenicity*; D013752:Tetracycline / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D013752:Tetracycline