The cardiac copper chaperone proteins Sco1 and CCS are up-regulated, but Cox 1 and Cox4 are down-regulated, by copper deficiency.
Copper is ferried in a cell complexed to chaperone proteins, and in the heart much copper is required for cytochrome c oxidase (Cox). It is not completely understood how copper status affects the levels of these proteins. Here we determined if dietary copper deficiency could up- or down-regulate select copper chaperone proteins and Cox subunits 1 and 4 in cardiac tissue of rats. Sixteen weanling male Long-Evans rats were randomized into treatment groups, one group receiving a copper-deficient diet (<1 mg Cu/kg diet) and one group receiving a diet containing adequate copper (6 mg Cu/kg diet) for 5 weeks. Hearts were removed, weighed, and non-myofibrillar proteins separated to analyze for levels of CCS, Sco1, Ctr1, Cox17, Cox1, and Cox4 by SDS-PAGE and Western blotting. No changes were observed in the concentrations of CTR1 and Cox17 between copper-adequate and copper-deficient rats. CCS and Sco1 were up-regulated and Cox1 and Cox4 were both down-regulated as a result of copper deficiency. These data suggest that select chaperone proteins and may be up-regulated, and Cox1 and 4 down-regulated, by a dietary copper deficiency, whereas others appear not to be affected by copper status.
Getz
Jean
J
Department of Human Nutrition, Kansas State University, 213 Justin Hall, Manhattan, KS 66506, USA. jeangetz@gmail.com
Lin
Dingbo
D
Medeiros
Denis M
DM
D000818:Animals; D006332:Cardiomegaly / Q000378:metabolism; D003300:Copper / Q000172:deficiency* / Q000378:metabolism*; D051545:Cyclooxygenase 1 / Q000378:metabolism*; D003576:Electron Transport Complex IV / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism*; D018832:Molecular Chaperones / Q000378:metabolism*; D051381:Rats; D020318:Rats, Long-Evans
C491961:Ccs protein, rat; D008565:Membrane Proteins; D018832:Molecular Chaperones; D003300:Copper; D051545:Cyclooxygenase 1; C496513:Ptgs1 protein, rat; D003576:Electron Transport Complex IV