title: NHANES monitoring of serum 25-hydroxyvitamin D: a roundtable summary.
abstract: A roundtable to discuss monitoring of serum 25-hydroxyvitamin D [25(OH)D] in the NHANES was held in late July 2009. Topics included the following: 1) options for dealing with assay fluctuations in serum 25(OH)D in the NHANES conducted between 1988 and 2006; 2) approaches for transitioning between the RIA used in the NHANES between 1988 and 2006 to the liquid chromatography tandem MS (LC-MS/MS) measurement procedure to be used in NHANES 2007 and later; 3) approaches for integrating the recently available standard reference material for vitamin D in human serum (SRM 972) from the National Institute of Standards and Technology (NIST) into the NHANES; 4) questions regarding whether the C-3 epimer of 25-hydroxyvitamin D3 [3-epi-25(OH)D3] should be measured in NHANES 2007 and later; and 5) identification of research and educational needs. The roundtable experts agreed that the NHANES data needed to be adjusted to control for assay fluctuations and offered several options for addressing this issue. The experts suggested that the LC-MS/MS measurement procedure developed by NIST could serve as a higher order reference measurement procedure. They noted the need for a commutability study for the recently released NIST SRM 972 across a range of measurement procedures. They suggested that federal agencies and professional organizations work with manufacturers to improve the quality and comparability of measurement procedures across all laboratories. The experts noted the preliminary nature of the evidence of the 3-epi-25(OH)D3 but felt that it should be measured in 2007 NHANES and later.
authors.affiliation: Office of Dietary Supplements, National Institutes of Health, Bethesda, MD 20892, USA. beth@yetley.com
authors.forename: Elizabeth A
authors.initials: EA
authors.lastname: Yetley
authors.forename: Christine M
authors.initials: CM
authors.lastname: Pfeiffer
authors.forename: Rosemary L
authors.initials: RL
authors.lastname: Schleicher
authors.forename: Karen W
authors.initials: KW
authors.lastname: Phinney
authors.forename: David A
authors.initials: DA
authors.lastname: Lacher
authors.forename: Sylvia
authors.initials: S
authors.lastname: Christakos
authors.forename: John H
authors.initials: JH
authors.lastname: Eckfeldt
authors.forename: James C
authors.initials: JC
authors.lastname: Fleet
authors.forename: George
authors.initials: G
authors.lastname: Howard
authors.forename: Andrew N
authors.initials: AN
authors.lastname: Hoofnagle
authors.forename: Siu L
authors.initials: SL
authors.lastname: Hui
authors.forename: Gary L
authors.initials: GL
authors.lastname: Lensmeyer
authors.forename: Joseph
authors.initials: J
authors.lastname: Massaro
authors.forename: Munro
authors.initials: M
authors.lastname: Peacock
authors.forename: Bernard
authors.initials: B
authors.lastname: Rosner
authors.forename: Donald
authors.initials: D
authors.lastname: Wiebe
authors.forename: Regan L
authors.initials: RL
authors.lastname: Bailey
authors.forename: Paul M
authors.initials: PM
authors.lastname: Coates
authors.forename: Anne C
authors.initials: AC
authors.lastname: Looker
authors.forename: Christopher
authors.initials: C
authors.lastname: Sempos
authors.forename: Clifford L
authors.initials: CL
authors.lastname: Johnson
authors.forename: Mary Frances
authors.initials: MF
authors.lastname: Picciano
mesh_terms: D015652:25-Hydroxyvitamin D 2 / Q000031:analogs & derivatives / Q000097:blood* / Q000737:chemistry / Q000592:standards; D002112:Calcifediol / Q000097:blood* / Q000737:chemistry / Q000592:standards; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D009749:Nutrition Surveys; D012015:Reference Standards; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry
chemical_list: D015652:25-Hydroxyvitamin D 2; D002112:Calcifediol