Scraping and stapling of end-grafted DNA chains by a bioadhesive spreading vesicle to reveal chain internal friction and topological complexity.
Stained end-grafted DNA molecules about 20 μm long are scraped away and stretched out by the spreading front of a bioadhesive vesicle. Tethered biotin ligands bind the vesicle bilayer to a streptavidin substrate, stapling the DNAs into frozen confinement paths. Image analysis of the stapled DNA gives access, within optical resolution, to the local stretching values of individual DNA molecules swept by the spreading front, and provides evidence of self-entanglements.
Nam
Gimoon
G
Institute of Fundamental Physics, Department of Physics, Sejong University, Seoul 143-743, Korea.
Hisette
Marie Laure
ML
Sun
Yuting Liang
YL
Gisler
Thomas
T
Johner
Albert
A
Thalmann
Fabrice
F
Schröder
André Pierre
AP
Marques
Carlos Manuel
CM
Lee
Nam-Kyung
NK
D000269:Adhesives / Q000737:chemistry*; D000818:Animals; D040761:Biomimetic Materials / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D017276:Friction; D021621:Imaging, Three-Dimensional; D009690:Nucleic Acid Conformation; D053835:Unilamellar Liposomes / Q000737:chemistry*
D000269:Adhesives; D053835:Unilamellar Liposomes; D004247:DNA