title: Rapid and reliable detection of LINE-1 hypomethylation using high-resolution melting analysis.
abstract: OBJECTIVE
  The aim of the present study was to evaluate the precision and reproducibility of the LINE-1 high-resolution melting (HRM) assay to detect LINE-1 hypomethylation.
  
  
  METHODS
  We first evaluated a methylated DNA dilution matrix and a panel of human cancer cell lines. We then applied this LINE-1 HRM assay to a set of 37 archival prostate cancer tissue samples.
  
  
  RESULTS
  Our LINE-1 HRM assay revealed small and reproducible run-to-run and bisulfite-to-bisulfite variations. As expected, we found a large variation in methylation levels between different cancer cell lines. All results were confirmed with MethyLight and pyrosequencing as indicated by the high correlation coefficient. Finally, we successfully applied the LINE-1 HRM assay to archival prostate cancer tissues.
  
  
  CONCLUSIONS
  The present LINE-1 HRM assay represents a novel, accurate, and cost-effective method to measure global hypomethylation, which makes it suitable for high- and low-throughput laboratories.
authors.affiliation: Division of Oncology, Department of Internal Medicine, Medical University of Graz, Graz, Austria.
authors.forename: Stefanie
authors.initials: S
authors.lastname: Stanzer
authors.forename: Marija
authors.initials: M
authors.lastname: Balic
authors.forename: Jasmin
authors.initials: J
authors.lastname: Strutz
authors.forename: Ellen
authors.initials: E
authors.lastname: Heitzer
authors.forename: Florian
authors.initials: F
authors.lastname: Obermair
authors.forename: Cornelia
authors.initials: C
authors.lastname: Hauser-Kronberger
authors.forename: Hellmut
authors.initials: H
authors.lastname: Samonigg
authors.forename: Nadia
authors.initials: N
authors.lastname: Dandachi
mesh_terms: D001681:Biological Assay / Q000379:methods*; D045744:Cell Line, Tumor; D019175:DNA Methylation; D004273:DNA, Neoplasm / Q000032:analysis*; D006801:Humans; D020084:Long Interspersed Nucleotide Elements; D009369:Neoplasms / Q000235:genetics / Q000378:metabolism; D009691:Nucleic Acid Denaturation
chemical_list: D004273:DNA, Neoplasm