title: Qualitative and quantitative determination of major saponins in Paris and Trillium by HPLC-ELSD and HPLC-MS/MS.
abstract: High-performance liquid chromatographic (HPLC) with evaporative light scattering detection (ELSD) and HPLC with electrospray ionization multistage tandem mass spectrometry (HPLC-ESI-MS(n)) were used to identify and quantify steroid saponins in Paris and Trillium plants. The content of the known saponins such as Paris I, II, III, V, VI, VII, H, gracillin and protodioscin in Paris and Trillium plants was determined simultaneously using the developed HPLC-ELSD method. Furthermore, other 12 steroid saponins were identified by HPLC-ESI(+/-)-MS(n) detection. In the end, a developed analytical procedure was proved to be a reliable and rapid method for the quality control of Paris and Trillium plants. In addition, the alternative resources for Paris yunnanensis used as a traditional Chinese medicine were discovered according to the hierarchical clustering analysis of the saponin fraction of these plants.
authors.affiliation: School of Pharmaceutical Science and Technology, Tianjin University, Weijin Road, Tianjin 300072, China.
authors.forename: Shuli
authors.initials: S
authors.lastname: Man
authors.forename: Wenyuan
authors.initials: W
authors.lastname: Gao
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Zhang
authors.forename: Jieyin
authors.initials: J
authors.lastname: Wang
authors.forename: Wanshun
authors.initials: W
authors.lastname: Zhao
authors.forename: Luqi
authors.initials: L
authors.lastname: Huang
authors.forename: Changxiao
authors.initials: C
authors.lastname: Liu
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D019668:Liliaceae / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D012503:Saponins / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*; D031446:Trillium / Q000737:chemistry*
chemical_list: D010936:Plant Extracts; D012503:Saponins