Bovine adipose triglyceride lipase is not altered and adipocyte fatty acid-binding protein is increased by dietary flaxseed.
In this paper, we report the full-length coding sequence of bovine ATGL cDNA and analyze its expression in bovine tissues. Similar to human, mouse, and pig ATGL sequences, bovine ATGL has a highly conserved patatin domain that is necessary for lipolytic function in mice and humans. This suggests that ATGL is functionally intact as a triglyceride lipase in cattle. Tissue distribution of ATGL gene expression was highest in fat and muscle (skeletal and cardiac) tissue, while protein expression was solely detectible in the adipose tissue. The effect of 109 days of flaxseed supplementation on ATGL and adipocyte fatty acid-binding protein (FABP4 or A-FABP, E-FABP or FABP5) expression was examined in Angus steers. Supplemented steers had greater triacylglycerol (TAG) content in the muscle compared with unsupplemented ones. Additionally, supplementation increased A-FABP expression and decreased stearoyl-CoA desaturase 1 (SCD-1) expression in muscle, while total ATGL expression was unaffected. In summary, supplementation of cattle rations with flaxseed increased muscle TAG concentrations attributed in part to increased expression of key enzymes involved in lipid trafficking (A-FABP) and metabolism (SCD-1).
Deiuliis
Jeffrey
J
Department of Animal Sciences and The Ohio State University Interdisciplinary Human Nutrition Program, The Ohio State University, Columbus, OH, USA.
Shin
Jonghyun
J
Murphy
Eric
E
Kronberg
Scott L
SL
Eastridge
Maurice L
ML
Suh
Yeunsu
Y
Yoon
Jong-Taek
JT
Lee
Kichoon
K
D000273:Adipose Tissue / Q000201:enzymology / Q000378:metabolism*; D000595:Amino Acid Sequence; D000821:Animal Feed; D000818:Animals; D002417:Cattle; D003001:Cloning, Molecular; D004032:Diet; D050556:Fatty Acid-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D019597:Flax / Q000502:physiology; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D006801:Humans; D008043:Linseed Oil / Q000494:pharmacology; D008049:Lipase / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D010802:Phylogeny; D014018:Tissue Distribution; D015854:Up-Regulation / Q000187:drug effects
D050556:Fatty Acid-Binding Proteins; D008043:Linseed Oil; D008049:Lipase