[Study of bone marrow stroma stem cells cell-sheet and endothelial progenitor cells cultured in vitro on allogenic demineralized bone matrix in mongrel].
OBJECTIVE
To investigate the growth situation of bone marrow stroma stem cells(BMSCs) cell-sheet and endothelial progenitor cells (EPCs) on demineralized bone matrix (DBM) and explore the effect of DBM/BMSCs cell-sheet and DBM/EPCs complex on the construction of functional and vascularized tissue-engineered bone in mongrel.


METHODS
BMSC cell-sheet was prepared with cell-sheet engineering approach, EPCs were isolated from canine bone marrow and DBM was prepared from homologous bone. BMSCs cell-sheet and EPCs were inoculated on DBM respectively and observed by inverted phase contrast microscope and scanning electron microscope. The porosity of DBM was measured.


RESULTS
The DBM/BMSCs cell-sheet and DBM/EPCs complex were successfully constructed. The BMSCs cell-sheet and EPCs adhered to DBM well and grew rapidly.


CONCLUSIONS
The BMSCs cell-sheet and EPCs have good biocompatibility with DBM. The complex of DBM/ BMSCs cell-sheet, DBM/EPCs could provide good conditions to acquire functional and vascularized tissue-engineered bone. Supported by National Natural Science Foundation of China (Grant No.30872896) and Natural Science Foundation of Shandong Province (Grant No.Y2008C77).
Tan
Shuai
S
Department of Oral and Maxillofacial Surgery, Affiliated Hospital of Medical College, Qingdao University, Qingdao 266003, Shandong Province, China. tan_shuai@hotmail.com
Li
Ning-yi
NY
Jing
Heng
H
Gao
Zhen-hua
ZH
Chen
Li-qiang
LQ
D001853:Bone Marrow; D001854:Bone Marrow Cells; D001857:Bone Matrix; D001842:Bone and Bones; D066026:Endothelial Progenitor Cells; D006801:Humans; D066298:In Vitro Techniques; D059630:Mesenchymal Stem Cells; D010012:Osteogenesis; D023822:Tissue Engineering; D054457:Tissue Scaffolds