Analysis of human cytomegalovirus glycoprotein N genotypes in Chinese hematopoietic stem cell transplant recipients.
Human cytomegalovirus (HCMV) genomic polymorphisms have been used to investigate correlations between virus variants and clinical characteristics. We explored the distribution of HCMV glycoprotein N (gN) genotypes and their roles relative to clinical features in a population of Chinese hematopoietic stem cell transplant (HSCT) recipients. This prospective analysis involved HCMV clinical isolates obtained from 102 HSCT patients. Real-time quantitative PCR and PCR-based restriction fragment length polymorphism analysis were applied for the determination of viral loads and gN genotypes. The distribution of HCMV gN genotypes was as follows: gN1, 6/102 (5.9%); gN2, 10/102 (9.8%); gN3a, 17/102 (16.7%); gN3b, 5/102 (4.9%); gN4a, 12/102 (11.7%); gN4b, 9/102 (8.8%); gN4d, 2/102 (2.0%); and mixtures, 41/102 (40.2%). No particular HCMV gN genotype was significantly associated with specific clinical characteristics. The HCMV gN3a genotype was the most prevalent among Chinese HSCT recipients, but HCMV gN genotypes may have no correlation with clinical features in HSCT patients.
Xia
Chang-sheng
CS
Department of Clinical Laboratory, Peking University People's Hospital, Xicheng District, Beijing, China.
Zhang
Zheng
Z
D000998:Antiviral Agents / Q000627:therapeutic use; D002681:China; D003587:Cytomegalovirus / Q000235:genetics* / Q000378:metabolism; D003586:Cytomegalovirus Infections / Q000209:etiology / Q000821:virology*; D004279:DNA, Viral / Q000302:isolation & purification; D005260:Female; D017245:Foscarnet / Q000627:therapeutic use; D015774:Ganciclovir / Q000627:therapeutic use; D014644:Genetic Variation; D005838:Genotype; D018380:Hematopoietic Stem Cell Transplantation / Q000009:adverse effects*; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D014759:Viral Envelope Proteins / Q000235:genetics* / Q000378:metabolism; D019562:Viral Load
D000998:Antiviral Agents; D004279:DNA, Viral; D014759:Viral Envelope Proteins; C417883:glycoprotein N, Human cytomegalovirus; D017245:Foscarnet; D015774:Ganciclovir