MicroRNAs: tiny targets for engineering CHO cell phenotypes?
The ability of microRNAs to influence gene expression is now recognized as a fundamental layer of regulation within the cell. MicroRNAs have a major impact on most biological processes and have generated considerable interest as potential biomarkers as well as therapeutic or engineering targets. In this review we provide a brief overview of their biogenesis, genomic organization and mode of action, followed by a description of the methods and approaches to studying their expression. We go on to consider some of the approaches to utilizing them as tools and their potential application in the bioprocessing area, with particular emphasis on Chinese hamster ovary cell engineering.
Barron
Niall
N
National Institute for Cellular Biotechnology, Dublin City University, Dublin 9, Ireland. niall.barron@dcu.ie
Sanchez
Noelia
N
Kelly
Paul
P
Clynes
Martin
M
D000818:Animals; D016466:CHO Cells; D018929:Cell Culture Techniques; D006224:Cricetinae; D003412:Cricetulus; D005786:Gene Expression Regulation; D018390:Gene Targeting / Q000379:methods*; D005818:Genetic Engineering / Q000379:methods*; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism*; D010641:Phenotype; D011994:Recombinant Proteins / Q000096:biosynthesis
D035683:MicroRNAs; D011994:Recombinant Proteins