A simple; efficient and environmentally friendly method for the extraction of pesticides from onion by matrix solid-phase dispersion with liquid chromatography-tandem mass spectrometric detection.
An evaluation of the extraction of pesticides from onion by matrix solid-phase dispersion (MSPD) with the determination by liquid chromatography tandem mass spectrometry using electrospray as the ionization source (LC-ESI-MS/MS) was carried out. The performance of different sorbents, including reused C18 bonded silica, was evaluated. Different parameters affecting the extraction efficiency were evaluated, such as the type and amount of sorbent, the time of interaction after the fortification step, the time of sample dispersion and the elution solvent. The matrix effect regarding the recovery of the pesticides by MSPD was also investigated. The best results were obtained using 0.5 g of sample, 1.0 g reused C18, interaction time of 1 h, dispersion time of 5 min, and acetonitrile as the elution solvent. The method was validated by the fortification of the onion sample, free of pesticides, at different concentration levels (0.01, 0.1 and 1.0 mg kg(-1)). Average recoveries ranged from 78.3 to 120.4% and relative standard deviation below 20% was obtained. Detection and quantification limits ranged from 0.003 to 0.03 mg kg(-1) and from 0.01 to 0.1 mg kg(-1), respectively.
Rodrigues
Sherol Acosta
SA
Post-graduation Program in Technological and Environmental Chemistry, Escola de Química e Alimentos, Universidade Federal do Rio Grande, Av Itália, km 8, s/n, Rio Grande, Rio Grande do Sul State, 96201-900, Brazil.
Caldas
Sergiane Souza
SS
Primel
Ednei Gilberto
EG
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D019697:Onions / Q000737:chemistry*; D010575:Pesticides / Q000032:analysis* / Q000302:isolation & purification; D012822:Silicon Dioxide / Q000737:chemistry; D052616:Solid Phase Extraction / Q000379:methods*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D010575:Pesticides; D012822:Silicon Dioxide