title: Functional polymorphism of the MMP-1 promoter (-1607 1G/2G) in potentially malignant and malignant head and neck lesions in an Indian population.
abstract: Matrix metalloproteinases (MMP) are a family of zinc-dependent proteases that degrade the entire component of the extracellular matrix. Our study explores the association of the MMP1 gene promoter (-1607 1G/2G) polymorphisms in oral submucous fibrosis (OSMF) and head and neck squamous cell carcinoma (HNSCC) in an Indian population. The MMP1 single-nucleotide polymorphism (SNP) was genotyped by polymerase chain reaction-restriction fragment length polymorphism analysis in 412 patients with OSMF, 422 with HNSCC and 426 controls. Our results showed that the frequency of 1G/2G or 2G/2G promoter genotypes having the 2G allele is associated with higher enzymatic activity and significantly increases in OSMF (p<0.001) and HNSCC cases (p<0.00). In this study, results concluded that SNPs in the MMP1 promoter region may be associated with susceptibility to OSMF as well as HNSCC in an Indian population and addiction habits such as areca nut chewing and alcohol abuse may enhance the expression of the 2G allele of MMP1 genes in OSMF and HNSCC cases.
authors.affiliation: Centre for Biotechnology, University of Allahabad, India.
authors.forename: Ajay Kumar
authors.initials: AK
authors.lastname: Chaudhary
authors.forename: Shruti
authors.initials: S
authors.lastname: Pandya
authors.forename: Ravi
authors.initials: R
authors.lastname: Mehrotra
authors.forename: Alok C
authors.initials: AC
authors.lastname: Bharti
authors.forename: Sachin
authors.initials: S
authors.lastname: Jain
authors.forename: Mamta
authors.initials: M
authors.lastname: Singh
mesh_terms: D000328:Adult; D001483:Base Sequence; D014408:Biomarkers, Tumor / Q000235:genetics*; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D016022:Case-Control Studies; D017931:DNA Primers; D005260:Female; D006258:Head and Neck Neoplasms / Q000235:genetics*; D006801:Humans; D007194:India; D008297:Male; D020781:Matrix Metalloproteinase 1 / Q000235:genetics*; D008875:Middle Aged; D009914:Oral Submucous Fibrosis / Q000235:genetics*; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic
chemical_list: D014408:Biomarkers, Tumor; D017931:DNA Primers; D020781:Matrix Metalloproteinase 1