Effects of histamine on light responses of amacrine cells in tiger salamander retina.
Using immunofluorescence, we showed that histamine receptor 1 is expressed by horizontal cell axons and a subset of amacrine cells in the tiger salamander retina. The effects of histamine on light responses of amacrine cells were studied in slice preparations. Histamine modulated the light responses of many salamander amacrine cells, depending upon the morphological type. The most pronounced effects of histamine were decreases in the light responses of broadly stratified amacrine cells, particularly those having medium-sized dendritic field diameters. To determine whether the effects of histamine were direct, Co(++) was substituted for Ca(++) in the extracellular medium to block synaptic transmission. Histamine still affected broadly stratified amacrine cells, but not narrowly stratified amacrine cells under these conditions. Taken together, these findings suggest that inhibitory interactions between strata of the IPL and within the classical receptive fields of the ganglion cells would be particularly sensitive to histamine released from retinopetal axons.
Yu
Yongchun
Y
Department of Neurobiology and Anatomy, University of Texas Medical School at Houston, Houston, TX 77225, USA.
Satoh
Hiromasa
H
Vila
Alejandro
A
Wu
Samuel M
SM
Marshak
David W
DW
D025042:Amacrine Cells / Q000502:physiology*; D000818:Animals; D005455:Fluorescent Antibody Technique; D006632:Histamine / Q000502:physiology*; D008027:Light; D008564:Membrane Potentials; D012160:Retina / Q000166:cytology / Q000502:physiology*; D014562:Caudata
D006632:Histamine