Engineered superparamagnetic Mn0.5Zn0.5Fe2O4 nanoparticles as a heat shock protein induction agent for ocular neuroprotection in glaucoma.
Ocular neuroprotection induced by localized heat shock proteins (HSPs) has been paid considerable attention as an efficacious treatment modality for glaucoma. However, the current clinical approaches to induce HSPs in the retinal ganglion cells (RGCs) are limited due to undesirable side effects. Here, we present that the induction of HSPs by local magnetic hyperthermia using engineered superparamagnetic Mn(0.5)Zn(0.5)Fe(2)O(4) nanoparticle agents (EMZF-SPNPAs) with a 5.5 nm mean particle size is promisingly feasible for a physiologically tolerable ocular neuroprotection modality. The sufficiently high specific absorption rate (SAR) (∼256.4 W/g in an agar solution) achieved at the biologically safe range of applied AC magnetic field and frequency as well as the superior biocompatibility of EMZF-SPNPA, which were confirmed from both in-vitro and in-vivo animal pilot studies, allowing it to be considered as a potential localized HSPs agent. Furthermore, the successful demonstration of a newly designed infusion technique, which diffuses the EMZF-SPNPAs through the vitreous body to the retina in a rat eye, more strongly verified the promises of this biotechnical approach to the ocular neuroprotection modality in glaucoma clinics.
Jeun
Minhong
M
Department of Electrical and Computer Engineering, National University of Singapore, Singapore.
Jeoung
Jin Wook
JW
Moon
Seungje
S
Kim
Yu Jeong
YJ
Lee
Sanghoon
S
Paek
Sun Ha
SH
Chung
Kyung-Won
KW
Park
Ki Ho
KH
Bae
Seongtae
S
D000818:Animals; D001672:Biocompatible Materials / Q000009:adverse effects / Q000737:chemistry* / Q000627:therapeutic use*; D002460:Cell Line; D005901:Glaucoma / Q000188:drug therapy* / Q000378:metabolism*; D006360:Heat-Shock Proteins / Q000378:metabolism*; D008280:Magnetics; D008297:Male; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000009:adverse effects / Q000737:chemistry* / Q000627:therapeutic use* / Q000648:ultrastructure; D051381:Rats; D017207:Rats, Sprague-Dawley; D012165:Retinal Ganglion Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D014822:Vitreous Body / Q000378:metabolism
D001672:Biocompatible Materials; D006360:Heat-Shock Proteins