title: A mechanistic view of the role of E3 in sumoylation.
abstract: Sumoylation, the covalent attachment of SUMO (Small Ubiquitin-Like Modifier) to proteins, differs from other Ubl (Ubiquitin-like) pathways. In sumoylation, E2 ligase Ubc9 can function without E3 enzymes, albeit with lower reaction efficiency. Here, we study the mechanism through which E3 ligase RanBP2 triggers target recognition and catalysis by E2 Ubc9. Two mechanisms were proposed for sumoylation. While in both the first step involves Ubc9 conjugation to SUMO, the subsequent sequence of events differs: in the first E2-SUMO forms a complex with the target and E3, followed by SUMO transfer to the target. In the second, Ubc9-SUMO binds to the target and facilitates SUMO transfer without E3. Using dynamic correlations obtained from explicit solvent molecular dynamic simulations we illustrate the key roles played by allostery in both mechanisms. Pre-existence of conformational states explains the experimental observations that sumoylation can occur without E3, even though at a reduced rate. Furthermore, we propose a mechanism for enhancement of sumoylation by E3. Analysis of the conformational ensembles of the complex of E2 conjugated to SUMO illustrates that the E2 enzyme is already largely pre-organized for target binding and catalysis; E3 binding shifts the equilibrium and enhances these pre-existing populations. We further observe that E3 binding regulates allosterically the key residues in E2, Ubc9 Asp100/Lys101 E2, for the target recognition.
authors.affiliation: Polymer Research Center & Chemical Engineering Department, Bogazici University, Istanbul, Turkey.
authors.forename: Melda
authors.initials: M
authors.lastname: Tozluoğlu
authors.forename: Ezgi
authors.initials: E
authors.lastname: Karaca
authors.forename: Ruth
authors.initials: R
authors.lastname: Nussinov
authors.forename: Türkan
authors.initials: T
authors.lastname: Haliloğlu
mesh_terms: D000494:Allosteric Regulation; D001665:Binding Sites; D006801:Humans; D018832:Molecular Chaperones / Q000737:chemistry; D008968:Molecular Conformation; D056004:Molecular Dynamics Simulation; D028861:Nuclear Pore Complex Proteins / Q000737:chemistry; D025841:Small Ubiquitin-Related Modifier Proteins / Q000737:chemistry; D058207:Sumoylation; D044763:Ubiquitin-Conjugating Enzymes / Q000502:physiology; D044767:Ubiquitin-Protein Ligases / Q000502:physiology*; D000098843:Ubiquitin-Conjugating Enzyme UBC9
chemical_list: D018832:Molecular Chaperones; D028861:Nuclear Pore Complex Proteins; D025841:Small Ubiquitin-Related Modifier Proteins; C094493:ran-binding protein 2; D044763:Ubiquitin-Conjugating Enzymes; D044767:Ubiquitin-Protein Ligases; D000098843:Ubiquitin-Conjugating Enzyme UBC9