title: Effect of doxycycline on postoperative scarring after trabeculectomy in an experimental rabbit model.
abstract: OBJECTIVE
  To investigate effectiveness of doxycycline after trabeculectomy in rabbits by evaluating bleb appearance, intraocular pressure, and levels of matrix metalloproteinase-1, -2, -3, and -9 and tissue inhibitors of metalloproteinase (TIMP)-1 and -2 in the subconjunctival (sc) area.
  
  
  METHODS
  Twenty-nine New Zealand White rabbits were assigned into 1 of 6 groups as follows: topical doxycycline (0.1%), postoperative sc injection of doxycycline (100 mg/2 mL), and intraoperative mitomycin-C (MMC) (0.2 mg/mL) and their respective control groups.
  
  
  RESULTS
  There was significant difference between intraocular pressure in the case groups, but there was no significant difference in topical doxycycline and MMC groups during the follow up. In the topical doxycycline group, levels of TIMP-1 and perifericTIMP-1 were higher and levels of perifericMMP-2 and inflammation were lower than their controls. In the sc doxycycline group, peripheral inflammation was higher than in the corresponding control. Only peripheral inflammation was significantly different between case groups, with the highest level in sc and the lowest level in MMC groups. Further, topical doxycycline group showed no significant difference in bleb appearance or peripheral inflammation compared with MMC group. Conjunctival burn and corneal vascularization were detected only in the sc doxycycline group.
  
  
  CONCLUSIONS
  Topical doxycycline is more effective than sc doxycycline but is similar to MMC, and it can be an alternative to MMC in trabeculectomy in rabbits.
authors.affiliation: Ulucanlar Eye Education and Research Hospital , Ankara, Turkey. eminesentr@yahoo.com
authors.forename: Emine
authors.initials: E
authors.lastname: Sen
authors.forename: Melike
authors.initials: M
authors.lastname: Balikoglu-Yilmaz
authors.forename: Sibel
authors.initials: S
authors.lastname: Bozdag-Pehlivan
authors.forename: Nuran
authors.initials: N
authors.lastname: Sungu
authors.forename: Fatma Nur
authors.initials: FN
authors.lastname: Aksakal
authors.forename: Ayse
authors.initials: A
authors.lastname: Altinok
authors.forename: Tulay
authors.initials: T
authors.lastname: Tuna
authors.forename: Nursen
authors.initials: N
authors.lastname: Unlu
authors.forename: Huseyin
authors.initials: H
authors.lastname: Ustun
authors.forename: Gultekin
authors.initials: G
authors.lastname: Koklu
authors.forename: Faruk
authors.initials: F
authors.lastname: Öztürk
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology; D001768:Blister / Q000473:pathology / Q000601:surgery; D002921:Cicatrix / Q000150:complications* / Q000473:pathology; D003228:Conjunctiva / Q000187:drug effects / Q000473:pathology / Q000601:surgery; D004318:Doxycycline / Q000494:pharmacology*; D005260:Female; D005901:Glaucoma / Q000188:drug therapy / Q000473:pathology / Q000601:surgery; D007249:Inflammation / Q000378:metabolism; D007429:Intraocular Pressure / Q000187:drug effects; D007430:Intraoperative Care; D045726:Metalloproteases / Q000494:pharmacology; D016685:Mitomycin / Q000494:pharmacology; D023421:Models, Animal; D011184:Postoperative Period; D011817:Rabbits; D019714:Tissue Inhibitor of Metalloproteinases / Q000494:pharmacology; D014130:Trabeculectomy; D014945:Wound Healing / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D000903:Antibiotics, Antineoplastic; D019714:Tissue Inhibitor of Metalloproteinases; D016685:Mitomycin; D045726:Metalloproteases; D004318:Doxycycline