title: Comparison of indirect and direct quantification of glycidol fatty acid ester in edible oils.
abstract: Two different methods for determining the levels of glycidol fatty acid esters (GEs) in edible oil-the German official indirect method and the direct LC-MS method-are compared. In some cases, the indirect method showed lower GE levels than the direct method. This was investigated using model studies, which revealed two possible causative factors during the acid treatment of the indirect method: (1) incomplete elimination of GE in oil that was high in GEs initially and (2) generation of GEs and/or its relevant compounds in oil that was rich in partial acylglycerol. Both these factors contributed to the subsequent underestimation of GE levels. The above technical limitations of the indirect method found in the present study has led to the inference that the direct method can more precisely determine the GE levels for a wider range of fats and oil products than the indirect method.
authors.affiliation: Tokyo Research Laboratories, Kao Corporation, Tokyo, Japan. shimizu.masao@kao.co.jp
authors.forename: Masao
authors.initials: M
authors.lastname: Shimizu
authors.forename: Naoto
authors.initials: N
authors.lastname: Kudo
authors.forename: Hiroki
authors.initials: H
authors.lastname: Shiro
authors.forename: Koichi
authors.initials: K
authors.lastname: Yasunaga
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Masukawa
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Katsuragi
authors.forename: Takeshi
authors.initials: T
authors.lastname: Yasumasu
mesh_terms: D002853:Chromatography, Liquid; D004852:Epoxy Compounds / Q000032:analysis*; D013058:Mass Spectrometry; D015394:Molecular Structure; D010938:Plant Oils / Q000737:chemistry*; D010945:Plants, Edible / Q000737:chemistry*; D013237:Stereoisomerism
chemical_list: D004852:Epoxy Compounds; D010938:Plant Oils; C020344:glycidyl ethers