title: Gene manipulation of chick embryos in vitro, early chick culture, and long survival in transplanted eggs.
abstract: We introduce a revolutionary gene transfer system in chick: transfect chick embryos at early developmental stage by electroporation in vitro, Early Chick (EC) culture, and transplant to the egg to let the embryo survive until E5.5. Referring to the fate map, we could target the tissues of transfection, or transfect large areas of the embryo. We could get tissue-specific expression of a transgene by tissue-specific promoter. This method is very convenient and rapid, but allows us to get stable expression of the transgene in combination with transposon system.
authors.affiliation: Department of Molecular Neurobiology, Graduate School of Life Sciences and Institute of Development, Aging and Cancer, Tohoku University, Aoba-ku, Sendai 980-8575, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Tanaka
authors.forename: Hidekiyo
authors.initials: H
authors.lastname: Harada
authors.forename: Kodai
authors.initials: K
authors.lastname: Ito
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Ogura
authors.forename: Harukazu
authors.initials: H
authors.lastname: Nakamura
mesh_terms: D000818:Animals; D002642:Chick Embryo / Q000196:embryology* / Q000378:metabolism / Q000637:transplantation*; D004251:DNA Transposable Elements / Q000235:genetics; D018274:Electroporation; D046149:Embryo Culture Techniques / Q000379:methods*; D009928:Organ Specificity; D010063:Ovum / Q000502:physiology*; D016019:Survival Analysis; D013997:Time Factors; D014162:Transfection / Q000379:methods*; D019076:Transgenes / Q000235:genetics
chemical_list: D004251:DNA Transposable Elements