title: Zirconia layer coated mesoporous silica microspheres used for highly specific phosphopeptide enrichment.
abstract: Zirconia layer coated mesoporous silica microspheres with mesostructured cellular foams (MCFs) were prepared by NH(3)/water vapor-induced internal hydrolysis method. Zirconia layer coated MCF microspheres were characterized by SEM, XRD, N(2) sorption, UV, and chromatographic analysis, and explored for enrichment of phosphopeptides. ZrO(2)/MCF microspheres in solid-phase extraction (SPE) mode demonstrated much higher selectivity and higher efficiency towards phosphopeptide enrichment than bulk ZrO(2) particles. In particular, the selectivities of ZrO(2)/MCF microspheres towards multi-phosphopeptides are even higher than that of the widely used commercial TiO(2) microparticles. The ZrO(2)/MCF microspheres were also applied to enrich endogenous phosphopeptides from human serum, and twelve endogenous phosphorylated peptides could be specifically enriched.
authors.affiliation: Key Lab of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian, China.
authors.forename: Huihui
authors.initials: H
authors.lastname: Wan
authors.forename: Jingyu
authors.initials: J
authors.lastname: Yan
authors.forename: Long
authors.initials: L
authors.lastname: Yu
authors.forename: Xiuli
authors.initials: X
authors.lastname: Zhang
authors.forename: Xingya
authors.initials: X
authors.lastname: Xue
authors.forename: Xiuling
authors.initials: X
authors.lastname: Li
authors.forename: Xinmiao
authors.initials: X
authors.lastname: Liang
mesh_terms: D006801:Humans; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D010748:Phosphopeptides / Q000097:blood / Q000302:isolation & purification*; D010766:Phosphorylation; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry*; D052616:Solid Phase Extraction; D021241:Spectrometry, Mass, Electrospray Ionization; D013499:Surface Properties; D014961:X-Ray Diffraction; D015040:Zirconium / Q000737:chemistry*
chemical_list: D010748:Phosphopeptides; D012822:Silicon Dioxide; D015040:Zirconium; C028541:zirconium oxide