title: Intestinal crypt homeostasis results from neutral competition between symmetrically dividing Lgr5 stem cells.
abstract: Intestinal stem cells, characterized by high Lgr5 expression, reside between Paneth cells at the small intestinal crypt base and divide every day. We have carried out fate mapping of individual stem cells by generating a multicolor Cre-reporter. As a population, Lgr5(hi) stem cells persist life-long, yet crypts drift toward clonality within a period of 1-6 months. We have collected short- and long-term clonal tracing data of individual Lgr5(hi) cells. These reveal that most Lgr5(hi) cell divisions occur symmetrically and do not support a model in which two daughter cells resulting from an Lgr5(hi) cell division adopt divergent fates (i.e., one Lgr5(hi) cell and one transit-amplifying [TA] cell per division). The cellular dynamics are consistent with a model in which the resident stem cells double their numbers each day and stochastically adopt stem or TA fates. Quantitative analysis shows that stem cell turnover follows a pattern of neutral drift dynamics.
authors.affiliation: Hubrecht Institute, KNAW and University Medical Center Utrecht, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands.
authors.forename: Hugo J
authors.initials: HJ
authors.lastname: Snippert
authors.forename: Laurens G
authors.initials: LG
authors.lastname: van der Flier
authors.forename: Toshiro
authors.initials: T
authors.lastname: Sato
authors.forename: Johan H
authors.initials: JH
authors.lastname: van Es
authors.forename: Maaike
authors.initials: M
authors.lastname: van den Born
authors.forename: Carla
authors.initials: C
authors.lastname: Kroon-Veenboer
authors.forename: Nick
authors.initials: N
authors.lastname: Barker
authors.forename: Allon M
authors.initials: AM
authors.lastname: Klein
authors.forename: Jacco
authors.initials: J
authors.lastname: van Rheenen
authors.forename: Benjamin D
authors.initials: BD
authors.lastname: Simons
authors.forename: Hans
authors.initials: H
authors.lastname: Clevers
mesh_terms: D000818:Animals; D019070:Cell Lineage; D002999:Clone Cells; D007421:Intestine, Small / Q000166:cytology*; D051379:Mice; D008954:Models, Biological; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology*
chemical_list: C493597:Lgr5 protein, mouse; D043562:Receptors, G-Protein-Coupled