Assessment of genetic diversity by simple sequence repeat markers among forty elite varieties in the germplasm for malting barley breeding.
The genetic diversity and relationship among 40 elite barley varieties were analyzed based on simple sequence repeat (SSR) genotyping data. The amplified fragments from SSR primers were highly polymorphic in the barley accessions investigated. A total of 85 alleles were detected at 35 SSR loci, and allelic variations existed at 29 SSR loci. The allele number per locus ranged from 1 to 5 with an average of 2.4 alleles per locus detected from the 40 barley accessions. A cluster analysis based on the genetic similarity coefficients was conducted and the 40 varieties were classified into two groups. Seven malting barley varieties from China fell into the same subgroup. It was found that the genetic diversity within the Chinese malting barley varieties was narrower than that in other barley germplasm sources, suggesting the importance and feasibility of introducing elite genotypes from different origins for malting barley breeding in China.
Wang
Jun-mei
JM
Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
Yang
Jian-ming
JM
Zhu
Jing-huan
JH
Jia
Qiao-jun
QJ
Tao
Yue-zhi
YZ
D001947:Breeding; D005819:Genetic Markers; D014644:Genetic Variation; D001467:Hordeum / Q000235:genetics*; D005810:Multigene Family; D012091:Repetitive Sequences, Nucleic Acid
D005819:Genetic Markers