Detection of gastric carcinoma-associated antigen MG7-Ag in human sera using surface plasmon resonance sensor.
BACKGROUND
MG7-Ag is a kind of gastric cancer-specific tumor-associated antigen and has been investigated to serve as a marker of gastric cancer for early diagnosis.


METHODS
Surface plasmon resonance (SPR) sensor was used for the detection of MG7-Ag in the sera of gastric cancer patients to develop an innovative, simple and rapid assay method for early diagnosis. The specific monoclonal MG7 antibodies were used as capture and detection receptors which were immobilized on the surface of SPR sensor chips for MG7-Ag identification in the human sera. The measurements include 9 cases of gastric cancer patients and 2 cases of healthy blood donors and a MKN45 cancer cell lysate solution sample for positive control.


RESULTS
The binding of MG7-Ag onto the sensor surface was observed from SPR spectra. The sera of most gastric cancer patients revealed much higher expression level of MG7-Ag than healthy human sera did in SPR measurement.


CONCLUSIONS
The initial results demonstrate that the SPR biosensor has the potential for its application in the early diagnosis of gastric cancer. However, more tests need to be done to confirm the detection limitation and the criterion for cancer risk evaluation in early diagnosis.
Fang
Xiangyi
X
Physics Department, School of Science, Xian Jiaotong University, Xi'an, PR China. xyfang@mail.xjtu.edu.cn
Tie
Jun
J
Xie
Yonghong
Y
Li
Quanjiang
Q
Zhao
Qingchuan
Q
Fan
Daiming
D
D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology; D000951:Antigens, Neoplasm / Q000097:blood* / Q000276:immunology; D014408:Biomarkers, Tumor / Q000097:blood* / Q000276:immunology; D015374:Biosensing Techniques / Q000379:methods; D006801:Humans; D013274:Stomach Neoplasms / Q000097:blood* / Q000276:immunology; D020349:Surface Plasmon Resonance / Q000379:methods*
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; C508753:MG7 antigen, human