Release kinetics and in vitro bioactivity of basic fibroblast growth factor: effect of the thickness of fibrous matrices.
In this study, we fabricated non-woven matrices using blends of polycaprolactone and gelatin with various spinning volumes to control the immobilized heparin content, which was ultimately intended to increase the immobilization efficiency of bFGF. The amount of bFGF on the heparin conjugated fibrous matrices depended on the thicknesses of the swollen matrices ranging from 35.4 ± 6.5 to 162.3 ± 14.0 ng and ≈90% of the bFGF was gradually released over a period of up to 56 d. The released bFGF enhanced the proliferation of human umbilical vein endothelial cells and human mesenchymal stem cells. In conclusion, our heparin-conjugated fibrous matrices have the potential to be used as a growth factor delivery system in tissue engineering applications.
Kim
Min Sup
MS
Department of Biomedical Engineering, Hanyang University, 17 Haengdang-dong, Seongdong-gu, Seoul 133-791, Korea.
Shin
Young Min
YM
Lee
Ji-Hye
JH
Kim
Sun I
SI
Nam
Young Soo
YS
Shin
Choongsoo S
CS
Shin
Heungsoo
H
D049109:Cell Proliferation; D002478:Cells, Cultured; D016503:Drug Delivery Systems; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects; D016222:Fibroblast Growth Factor 2 / Q000737:chemistry / Q000494:pharmacology*; D005780:Gelatin; D006493:Heparin / Q000737:chemistry; D006801:Humans; D055767:Immobilized Proteins / Q000737:chemistry / Q000494:pharmacology; D007700:Kinetics; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects; D057139:Nanofibers; D011091:Polyesters; D014471:Umbilical Veins / Q000166:cytology
D055767:Immobilized Proteins; D011091:Polyesters; D016222:Fibroblast Growth Factor 2; C016240:polycaprolactone; D005780:Gelatin; D006493:Heparin