title: Nanoencapsulation and characterization of Albizia chinensis isolated antioxidant quercitrin on PLA nanoparticles.
abstract: The plant isolated antioxidant quercitrin has been encapsulated on poly-d,l-lactide (PLA) nanoparticles by solvent evaporation method to improve the solubility, permeability and stability of this molecule. The size of quercitrin-PLA nanoparticles is 250±68nm whereas that PLA nanoparticles is 195 ± 55nm. The encapsulation efficiency of nanoencapsulated quercitrin evaluated by HPLC and antioxidant assay is 40%. The in vitro release kinetics of quercitrin under physiological condition reveals initial burst release followed by sustained release. Less fluorescence quenching is observed with equimolar concentration of PLA encapsulated quercitrin than free quercitrin. The presence of quercitrin specific peaks on FTIR of five times washed quercitrin loaded PLA nanoparticles provides an extra evidence for the encapsulation of quercitrin into PLA nanoparticles. These properties of quercitrin nanomedicine provide a new potential for the use of such less useful highly active antioxidant molecule towards the development of better therapeutic for intestinal anti-inflammatory effect and nutraceutical compounds.
authors.affiliation: Nanobiology Lab, Biotechnology Division, Institute of Himalayan Bioresource Technology, CSIR, Palampur 176061, HP, India.
authors.forename: Avnesh
authors.initials: A
authors.lastname: Kumari
authors.forename: Sudesh Kumar
authors.initials: SK
authors.lastname: Yadav
authors.forename: Yogesh B
authors.initials: YB
authors.lastname: Pakade
authors.forename: Vineet
authors.initials: V
authors.lastname: Kumar
authors.forename: Bikram
authors.initials: B
authors.lastname: Singh
authors.forename: Abha
authors.initials: A
authors.lastname: Chaudhary
authors.forename: Subhash Chandra
authors.initials: SC
authors.lastname: Yadav
mesh_terms: D031289:Albizzia / Q000737:chemistry*; D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification*; D001713:Biphenyl Compounds / Q000737:chemistry; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D016166:Free Radical Scavengers / Q000494:pharmacology; D018625:Microscopy, Atomic Force; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D010851:Picrates / Q000737:chemistry; D011091:Polyesters / Q000737:chemistry*; D011794:Quercetin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification; D012710:Serum Albumin, Bovine / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D010851:Picrates; D011091:Polyesters; D012710:Serum Albumin, Bovine; C012526:quercitrin; C033616:poly(lactide); D011794:Quercetin; C004931:1,1-diphenyl-2-picrylhydrazyl