title: Yeast elicitor-induced stomatal closure and peroxidase-mediated ROS production in Arabidopsis.
abstract: Yeast elicitor (YEL) induces stomatal closure. We investigated reactive oxygen species (ROS) production, nitric oxide (NO) production and [Ca(2+)](cyt) oscillations to clarify YEL signaling in Arabidopsis guard cells. YEL induced ROS accumulation in guard cells. A peroxidase inhibitor [salicylhydroxamic acid (SHAM)] inhibited the stomatal closure and the ROS accumulation, but neither the atrbohD atrbohF mutation nor an NADPH oxidase inhibitor [diphenylene iodonium chloride (DPI)] had any effect. An NO scavenger [2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO)] inhibited the YEL-induced stomatal closure and SHAM abolished NO production. YEL-elicited [Ca(2+)](cyt) oscillations were inhibited by SHAM but not by the atrbohD atrbohF mutation. These results indicate that YEL induces stomatal closure accompanied by ROS production mediated by peroxidases and NO production.
authors.affiliation: Graduate School of Natural Science and Technology, Division of Bioscience, Okayama University, 1-1-1 Tsushima-Naka, Okayama, 700-8530 Japan.
authors.forename: Md Atiqur Rahman
authors.initials: MA
authors.lastname: Khokon
authors.forename: Mohammad Anowar
authors.initials: MA
authors.lastname: Hossain
authors.forename: Shintaro
authors.initials: S
authors.lastname: Munemasa
authors.forename: Misugi
authors.initials: M
authors.lastname: Uraji
authors.forename: Yoshimasa
authors.initials: Y
authors.lastname: Nakamura
authors.forename: Izumi C
authors.initials: IC
authors.lastname: Mori
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Murata
mesh_terms: D017360:Arabidopsis / Q000201:enzymology / Q000378:metabolism* / Q000502:physiology; D002118:Calcium / Q000378:metabolism; D010544:Peroxidases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D015398:Signal Transduction; D015003:Yeasts / Q000378:metabolism*
chemical_list: D017382:Reactive Oxygen Species; D010544:Peroxidases; D002118:Calcium