Ottowia pentelensis sp. nov., a floc-forming betaproteobacterium isolated from an activated sludge system treating coke plant effluent.
A Gram-negative-staining, short-rod-shaped, floc-forming bacterium, designated strain RB3-7(T), was isolated from a laboratory-scale activated sludge system treating coke plant effluent. Comparative analysis of the 16S rRNA gene sequence demonstrated that the novel isolate was distantly related (≤ 95.8 % similarity) to Ottowia thiooxydans K11(T) within the family Comamonadaceae. Strain RB3-7(T) was catalase- and oxidase-positive and non-motile. The predominant fatty acids were C₁₆:₀, cyclo C₁₇:₀, C₁₈:₁ω7c and C₁₆:₁ω7c, and the major respiratory quinone was Q-8. The G+C content of the genomic DNA of strain RB3-7(T) was 68.5 mol%. On the basis of phenotypic, chemotaxonomic and molecular data, strain RB3-7(T) is considered to represent a novel species of the genus Ottowia, for which the name Ottowia pentelensis sp. nov. is proposed. The type strain is RB3-7(T) ( = DSM 21699(T) = NCAIM B 02336(T)).
Felföldi
Tamás
T
Department of Microbiology, Eötvös Loránd University, Pázmány Péter stny. 1/c, H-1117 Budapest, Hungary.
Kéki
Zsuzsa
Z
Department of Microbiology, Eötvös Loránd University, Pázmány Péter stny. 1/c, H-1117 Budapest, Hungary.
Sipos
Rita
R
Department of Microbiology, Eötvös Loránd University, Pázmány Péter stny. 1/c, H-1117 Budapest, Hungary.
Márialigeti
Károly
K
Department of Microbiology, Eötvös Loránd University, Pázmány Péter stny. 1/c, H-1117 Budapest, Hungary.
Tindall
Brian J
BJ
DSMZ - German Collection of Microorganisms and Cell Cultures, Inhoffenstrasse 7B, D-38124 Braunschweig, Germany.
Schumann
Peter
P
DSMZ - German Collection of Microorganisms and Cell Cultures, Inhoffenstrasse 7B, D-38124 Braunschweig, Germany.
Tóth
Erika M
EM
Department of Microbiology, Eötvös Loránd University, Pázmány Péter stny. 1/c, H-1117 Budapest, Hungary.
D015373:Bacterial Typing Techniques; D001482:Base Composition; D002374:Catalase / Q000378:metabolism; D016000:Cluster Analysis; D042621:Comamonadaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000378:metabolism; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D010088:Oxidoreductases; D002374:Catalase