title: The Staphylococcus aureus autoinducer-2 synthase LuxS is regulated by Ser/Thr phosphorylation.
abstract: The Staphylococcus aureus autoinducer-2 (AI-2) producer protein LuxS is phosphorylated by the Ser/Thr kinase Stk1 at a unique position, Thr14. The enzymatic activity of the phosphorylated isoform of LuxS was abrogated compared to that of nonphosphorylated LuxS, thus providing the first evidence of an AI-2-producing enzyme regulated by phosphorylation and demonstrating that S. aureus possesses an original and specific system for controlling AI-2 synthesis.
authors.affiliation: Institut de Biologie et Chimie des Protéines (IBCP UMR 5086), CNRS, Université Lyon 1, IFR128 BioSciences, Lyon-Gerland, 7 Passage du Vercors, Lyon Cedex 07, France.
authors.forename: Marie-Eve
authors.initials: ME
authors.lastname: Cluzel
authors.forename: Isabelle
authors.initials: I
authors.lastname: Zanella-Cléon
authors.forename: Alain J
authors.initials: AJ
authors.lastname: Cozzone
authors.forename: Klaus
authors.initials: K
authors.lastname: Fütterer
authors.forename: Bertrand
authors.initials: B
authors.lastname: Duclos
authors.forename: Virginie
authors.initials: V
authors.lastname: Molle
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D013437:Carbon-Sulfur Lyases / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D006714:Homoserine / Q000031:analogs & derivatives* / Q000096:biosynthesis; D007783:Lactones; D008954:Models, Biological; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment; D013211:Staphylococcus aureus / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D013912:Threonine / Q000378:metabolism; D037521:Virulence Factors / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D007783:Lactones; C091088:N-octanoylhomoserine lactone; D037521:Virulence Factors; D013912:Threonine; D006714:Homoserine; D017346:Protein Serine-Threonine Kinases; C546823:Stk1 protein, Staphylococcus aureus; D013437:Carbon-Sulfur Lyases; C117753:LuxS protein, Bacteria