title: Separation and characterization of forced degradation products of abacavir sulphate by LC-MS/MS.
abstract: Abacavir sulphate was subjected to forced degradation under the conditions of hydrolysis (acid, alkali and neutral), oxidation, photolysis and thermal stress as prescribed by ICH. Eight degradation products were formed and their separation was accomplished on Waters XTerra C₁₈ (250 mm x 4.6 mm, 5 μm) column using 20 mM ammonium acetate:acetonitrile as a mobile phase in gradient elution mode by LC. The degradation products were characterized by LC-MS/MS and its fragmentation pathways were proposed. No previous reports were found in the literature regarding the degradation behavior of abacavir sulphate.
authors.affiliation: Analytical Chemistry Division, D215, Discovery Laboratory, Indian Institute of Chemical Technology, Tarnaka, Hyderabad 500607, India. rnrao55@yahoo.com
authors.forename: R Nageswara
authors.initials: RN
authors.lastname: Rao
authors.forename: R Mastan
authors.initials: RM
authors.lastname: Vali
authors.forename: Bondigalla
authors.initials: B
authors.lastname: Ramachandra
authors.forename: S Satyanarayana
authors.initials: SS
authors.lastname: Raju
mesh_terms: D002853:Chromatography, Liquid; D015224:Dideoxynucleosides / Q000737:chemistry*; D017408:Guidelines as Topic / Q000592:standards; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007391:International Cooperation; D007700:Kinetics; D015394:Molecular Structure; D010084:Oxidation-Reduction; D010782:Photolysis; D015203:Reproducibility of Results; D018894:Reverse Transcriptase Inhibitors / Q000737:chemistry*; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*; D000099227:Abacavir
chemical_list: D015224:Dideoxynucleosides; D018894:Reverse Transcriptase Inhibitors; D000099227:Abacavir