title: Gene expression study of monocytes/macrophages during early foreign body reaction and identification of potential precursors of myofibroblasts.
abstract: Foreign body reaction (FBR), initiated by adherence of macrophages to biomaterials, is associated with several complications. Searching for mechanisms potentially useful to overcome these complications, we have established the signaling role of monocytes/macrophages in the development of FBR and the presence of CD34(+) cells that potentially differentiate into myofibroblasts. Therefore, CD68(+) cells were in vitro activated with fibrinogen and also purified from the FBR after 3 days of implantation in rats. Gene expression profiles showed a switch from monocytes and macrophages attracted by fibrinogen to activated macrophages and eventually wound-healing macrophages. The immature FBR also contained a subpopulation of CD34(+) cells, which could be differentiated into myofibroblasts. This study showed that macrophages are the clear driving force of FBR, dependent on milieu, and myofibroblast deposition and differentiation.
authors.affiliation: Laboratory of Experimental Cardiac Surgery, Department of Cardiovascular Diseases, KULeuven, Leuven, Belgium.
authors.forename: Lindsay
authors.initials: L
authors.lastname: Mesure
authors.forename: Geofrey
authors.initials: G
authors.lastname: De Visscher
authors.forename: Ilse
authors.initials: I
authors.lastname: Vranken
authors.forename: An
authors.initials: A
authors.lastname: Lebacq
authors.forename: Willem
authors.initials: W
authors.lastname: Flameng
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D004195:Disease Models, Animal; D005340:Fibrinogen / Q000276:immunology; D005549:Foreign-Body Reaction / Q000235:genetics* / Q000276:immunology / Q000503:physiopathology; D015870:Gene Expression; D020869:Gene Expression Profiling; D006801:Humans; D008264:Macrophages / Q000166:cytology / Q000276:immunology*; D008297:Male; D009000:Monocytes / Q000166:cytology / Q000276:immunology*; D058628:Myofibroblasts / Q000166:cytology* / Q000276:immunology; D051381:Rats; D017208:Rats, Wistar
chemical_list: D005340:Fibrinogen