Action of propolis and medications against Escherichia coli and endotoxin in root canals.
This study evaluated the action of propolis and intracanal medications against Escherichia coli and endotoxin. Forty-eight dental roots were contaminated with E. coli. The root canals were instrumented with propolis and divided into groups according to the type of intracanal medication: Ca(OH)(2), polymyxin B, or Ca(OH)(2) + 2% chlorhexidine gel. In the control group, saline solution was used without application of intracanal medication. Counts of colony-forming units were carried out and the endotoxin was quantified by the chromogenic Limulus amobocyte lysate assay. The results were evaluated by analysis of variance and the Dunn test (5%). Root canal irrigation with propolis was effective to completely eliminate E. coli and reduce the amount of endotoxins. All intracanal medications contributed to the significant decrease in endotoxins. Only intracanal medications may reduce the amount of endotoxins in the root canals. The greatest efficacy was observed for medications containing Ca(OH)(2).
Valera
Marcia Carneiro
MC
Department of Restorative Dentistry, School of Dentistry of São José dos Campos, São Paulo State University, São José dos Campos, Brazil. marcia@fosjc.unesp.br
da Rosa
Jucely Aparecida
JA
Maekawa
Lilian Eiko
LE
de Oliveira
Luciane Dias
LD
Carvalho
Cláudio Antonio Talge
CA
Koga-Ito
Cristiane Yumi
CY
Jorge
Antonio Olavo Cardoso
AO
D000704:Analysis of Variance; D000890:Anti-Infective Agents / Q000494:pharmacology*; D002126:Calcium Hydroxide / Q000494:pharmacology; D002710:Chlorhexidine / Q000494:pharmacology; D015169:Colony Count, Microbial; D003786:Dental Pulp Cavity / Q000382:microbiology*; D004338:Drug Combinations; D004731:Endotoxins / Q000032:analysis / Q000037:antagonists & inhibitors; D004926:Escherichia coli / Q000187:drug effects*; D006801:Humans; D008033:Limulus Test; D011112:Polymyxin B / Q000494:pharmacology; D011429:Propolis / Q000494:pharmacology*; D012388:Root Canal Irrigants / Q000494:pharmacology*
D000890:Anti-Infective Agents; D004338:Drug Combinations; D004731:Endotoxins; D012388:Root Canal Irrigants; D011429:Propolis; D011112:Polymyxin B; D002126:Calcium Hydroxide; D002710:Chlorhexidine