title: When one plus one equals more than two--a novel stain for renal biopsies is a combination of two classical stains.
abstract: Histologic evaluation of renal biopsies includes multiple ancillary stains, including Periodic acid-Schiff's (PAS) and Masson's trichrome (Trichrome). Herein we report an innovative double-stain, derived from two standard stains (PAS and Trichrome). This novel stain not only has advantages of both ancestor stains, but became more distinguishable and colorful, when basement membranes stain dark-violet, whereas the interstitial collagen remains blue. This allows the pathologist immediate estimation of the amount of collagen, tubular atrophy and the degree of interstitial fibrosis in one section. Using computer-based analysis, we confirmed that our innovative double-stain highlights interstitial collagen better than Trichrome stain alone. We strongly recommend renal pathologists to try this innovative stain in their practice.
authors.affiliation: Department of Pathology, Renal and Transplant Pathology Laboratory, The Ohio State University, Columbus, OH 43210, USA. sergey.brodsky@osumc.edu
authors.forename: Sergey V
authors.initials: SV
authors.lastname: Brodsky
authors.forename: Alia
authors.initials: A
authors.lastname: Albawardi
authors.forename: Anjali A
authors.initials: AA
authors.lastname: Satoskar
authors.forename: Gyongyi
authors.initials: G
authors.lastname: Nadasdy
authors.forename: Tibor
authors.initials: T
authors.lastname: Nadasdy
mesh_terms: D001391:Azo Compounds; D001706:Biopsy; D004801:Eosine Yellowish-(YS); D005355:Fibrosis; D006801:Humans; D007668:Kidney / Q000473:pathology*; D007674:Kidney Diseases / Q000175:diagnosis*; D008739:Methyl Green; D010503:Periodic Acid-Schiff Reaction; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D001391:Azo Compounds; C017768:trichrome stain; D008739:Methyl Green; D004801:Eosine Yellowish-(YS)