β-cyclodextrin cross-linked polymer as solid-phase extraction material coupled with the spectrophotometric method for the analysis of serum albumin.
The β-cyclodextrin cross-linked polymer (β-CDCP) was synthesized and used as solid-phase extraction material to preconcentrate/analysis bovine serum albumin (BSA) coupled with UV-vis spectroscopy. The method based on the complex (BSA-phenylfluorone (PF)) adsorbed on β-CDCP. Adsorption kinetics and various factors of the formation procedure of BSA-PF and its retention on β-CDCP were investigated. The linear range and detection limit (DL) was 20.0-200.0 and 0.03 mg/L, respectively. Moreover, the β-CDCP could be used repeatedly. The proposed method has been applied to analysis serum albumin with satisfactory results.
Wu
Min
M
Department of Chemistry, Yangzhou University, Yangzhou 225002, PR China.
Zhu
Xiashi
X
D000327:Adsorption; D002482:Cellulose / Q000138:chemical synthesis / Q000737:chemistry* / Q000493:pharmacokinetics / Q000494:pharmacology; D003432:Cross-Linking Reagents / Q000494:pharmacology; D003505:Cyclodextrins / Q000138:chemical synthesis / Q000737:chemistry* / Q000493:pharmacokinetics / Q000494:pharmacology; D000431:Ethanol / Q000494:pharmacology; D005452:Fluoresceins / Q000737:chemistry; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry; D015203:Reproducibility of Results; D012709:Serum Albumin / Q000032:analysis* / Q000737:chemistry; D012965:Sodium Chloride / Q000494:pharmacology; D052616:Solid Phase Extraction / Q000379:methods*; D013053:Spectrophotometry / Q000379:methods; D013696:Temperature; D047392:beta-Cyclodextrins / Q000737:chemistry* / Q000493:pharmacokinetics
D003432:Cross-Linking Reagents; D003505:Cyclodextrins; D005452:Fluoresceins; D011108:Polymers; D012709:Serum Albumin; D047392:beta-Cyclodextrins; C041953:cyclodextrin polymer; D000431:Ethanol; D012965:Sodium Chloride; C010159:fluorone Black; D002482:Cellulose; C031215:betadex