title: Aldolase A-HBsAg interaction and its effect on ultraviolet radiation induced apoptosis in 293FT cells.
abstract: OBJECTIVE
  Hepatitis B virus (HBV) infection poses great challenges to humans, claiming one million lives annually worldwide. Solid data have related HBV to hepatocellular carcinoma.
  
  
  METHODS
  In the present research, we verified the interaction between surface protein (HBs) encoded by HBV and aldolase A (ALDA) using yeast two-hybrid, mammalian two-hybrid, co-immunoprecipitation, GST pull-down and laser scanning confocal.
  
  
  RESULTS
  Anti-ALDA antibody precipitated Gal4-HBs fusion protein in the presence of HBs. Anti-HBs antibody precipitated p65ΔN-ALDA only in the presence of ALDA. Small HBs could be pulled down by GST-ALDA. Cells transfected with pCMV-AD-ALDA showed a protection from ultraviolet radiation-induced apoptosis (21.3% ± 1.3% for ALDA, 35.4% ± 2.1% for control, P < 0.05).
  
  
  CONCLUSIONS
  An interaction does exist between ALDA and HBs. The S region within HBs is sufficient for binding ALDA. In addition, ALDA conferred protection to ultraviolet radiation-induced apoptosis, and this effect was enhanced by the interaction between HBs and ALDA.
authors.affiliation: Division of Gastroenterology, Zhongshan Hospital Xiamen University, and Gastroenterology Institute of Xiamen University, and Gastroenterology Center of Xiamen, Xiamen, Fujian Province, China.
authors.forename: Jin-Shui
authors.initials: JS
authors.lastname: Pan
authors.forename: Fei
authors.initials: F
authors.lastname: Zhou
authors.forename: Chen-Xi
authors.initials: CX
authors.lastname: Xie
authors.forename: Jia-Yan
authors.initials: JY
authors.lastname: Cai
authors.forename: Jian-Min
authors.initials: JM
authors.lastname: Chen
authors.forename: Zhi-Ping
authors.initials: ZP
authors.lastname: Zhang
authors.forename: Jing
authors.initials: J
authors.lastname: Dong
authors.forename: Hong-Zhi
authors.initials: HZ
authors.lastname: Xu
authors.forename: Hua-Xiu
authors.initials: HX
authors.lastname: Shi
authors.forename: Jian-Lin
authors.initials: JL
authors.lastname: Ren
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000276:immunology / Q000528:radiation effects*; D015153:Blotting, Western; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D002460:Cell Line; D006224:Cricetinae; D004247:DNA / Q000032:analysis; D004273:DNA, Neoplasm / Q000235:genetics; D005434:Flow Cytometry; D005634:Fructose-Bisphosphate Aldolase / Q000276:immunology*; D006514:Hepatitis B Surface Antigens / Q000276:immunology*; D019694:Hepatitis B, Chronic / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D018613:Microscopy, Confocal; D016133:Polymerase Chain Reaction; D014466:Ultraviolet Rays
chemical_list: D004273:DNA, Neoplasm; D006514:Hepatitis B Surface Antigens; D004247:DNA; D005634:Fructose-Bisphosphate Aldolase