title: Portable optoelectronic biosensing platform for identification of mycobacteria from the Mycobacterium tuberculosis complex.
abstract: In this paper we report on the fabrication and performance of a portable and low cost optoelectronic platform integrating a double color tuned light emitting diode as light source, an amorphous/nanocrystalline silicon photodetector with a flat spectral response in the wavelength range from 520 nm to 630 nm and integrated electronic for signal acquisition and conditioning constituted by current to voltage converter, a filter and an amplification stage, followed by an analog to digital converter, with appropriate software for full automation to minimize human error. Incorporation of the double color tuned light emitting diode provides for a simple yet innovative solution to signal acquisition independently from the light intensity and/or solution concentration, while considerably decreasing production costs. Detection based on Au-nanoprobes constitutes the biorecognition step and allowed identification of specific sequences of Mycobacterium tuberculosis complex, namely Mycobacterium bovis and M. tuberculosis in biological samples.
authors.affiliation: CENIMAT/I3N, Departamento de Ciência dos Materiais, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa and CEMOP-UNINOVA, 2829-516 Caparica, Portugal.
authors.forename: Leonardo Bione
authors.initials: LB
authors.lastname: Silva
authors.forename: Bruno
authors.initials: B
authors.lastname: Veigas
authors.forename: Gonçalo
authors.initials: G
authors.lastname: Doria
authors.forename: Pedro
authors.initials: P
authors.lastname: Costa
authors.forename: João
authors.initials: J
authors.lastname: Inácio
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Martins
authors.forename: Elvira
authors.initials: E
authors.lastname: Fortunato
authors.forename: Pedro V
authors.initials: PV
authors.lastname: Baptista
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D003124:Colorimetry / Q000295:instrumentation*; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics*; D004582:Electronics, Medical / Q000295:instrumentation; D004867:Equipment Design; D019544:Equipment Failure Analysis; D008029:Lighting / Q000295:instrumentation; D008904:Miniaturization; D009169:Mycobacterium tuberculosis / Q000235:genetics* / Q000302:isolation & purification*; D010783:Photometry / Q000295:instrumentation*
chemical_list: D004269:DNA, Bacterial