Kidins220/ARMS contributes to airway inflammation and hyper-responsiveness in OVA-sensitized mice.
BALB/c mice were sensitized and challenged with ovalbumin. We hypothesized that Kidins220/ARMS influences airway inflammation and hyper-responsiveness during allergic airway challenge, and assessed it by intranasal administration of anti-NGF antibody or anti-ARMS antibody to mice. Airway resistance was measured using a sealed whole-body plethysmograph. Total cell numbers and the percentage of different inflammatory cells in BALF were counted. Expression of IL-1β, IL-4 and TNF-α were determined by ELISA, and NF-κB activation determined by EMSA. Kidins220/ARMS expression was observed in ovalbumin-sensitized mice by immunofluorescence or western blotting. IL-1β, IL-4, and TNF-α were overexpressed and NF-κB activation increased after allergen challenge compared with controls. After treatment with anti-ARMS or anti-NGF, levels of IL-1β, IL-4 and TNF-α and NF-κB activation were reduced in comparison with those of ovalbumin-sensitized mice. These results suggest that NGF-mediated Kidins220/ARMS signaling participates in the pathogenesis of asthma, and contributes to airway inflammation and hyper-responsiveness in ovalbumin-sensitized mice.
Ni
Xiuqin
X
Department of Neurobiology, Institute of Respirotory Diseases, China Medical University, Shenyang 110001, China.
Li
Xing
X
Fang
Xiubin
X
Li
Ning
N
Cui
Wanpeng
W
Zhang
Baohui
B
Liu
Yuli
Y
D000818:Animals; D000906:Antibodies / Q000008:administration & dosage; D004195:Disease Models, Animal; D004789:Enzyme Activation / Q000187:drug effects; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D007249:Inflammation / Q000276:immunology; D053583:Interleukin-1beta / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D008565:Membrane Proteins / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D016328:NF-kappa B / Q000378:metabolism; D020932:Nerve Growth Factor / Q000276:immunology; D010047:Ovalbumin / Q000276:immunology*; D012130:Respiratory Hypersensitivity / Q000276:immunology* / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D000906:Antibodies; D053583:Interleukin-1beta; C544017:Kidins220 protein, mouse; D008565:Membrane Proteins; D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha; D015847:Interleukin-4; D010047:Ovalbumin; D020932:Nerve Growth Factor