title: RT-PCR assays for seven serotypes of epizootic haemorrhagic disease virus & their use to type strains from the Mediterranean region and North America.
abstract: Epizootic haemorrhagic disease virus (EHDV) infects wild ruminants, causing a frequently fatal haemorrhagic disease. However, it can also cause bluetongue-like disease in cattle, involving significant levels of morbidity and mortality, highlighting a need for more rapid and reliable diagnostic assays. EHDV outer-capsid protein VP2 (encoded by genome-segment 2 [Seg-2]) is highly variable and represents the primary target for neutralising antibodies generated by the mammalian host. Consequently VP2 is also the primary determinant of virus "serotype", as identified in virus neutralisation tests (VNT). Although previous reports have indicated eight to ten EHDV serotypes, recent serological comparisons and molecular analyses of Seg-2 indicate only seven EHDV "types". Oligonucleotide primers were developed targeting Seg-2, for use in conventional RT-PCR assays to detect and identify these seven types. These assays, which are more rapid and sensitive, still show complete agreement with VNT and were used to identify recent EHDV isolates from the Mediterranean region and North America.
authors.affiliation: Vector Borne Diseases Programme, Institute for Animal Health, Pirbright Laboratory, Woking, Surrey, UK.
authors.forename: Narender S
authors.initials: NS
authors.lastname: Maan
authors.forename: Sushila
authors.initials: S
authors.lastname: Maan
authors.forename: Kyriaki
authors.initials: K
authors.lastname: Nomikou
authors.forename: Donna J
authors.initials: DJ
authors.lastname: Johnson
authors.forename: Mehdi
authors.initials: M
authors.lastname: El Harrak
authors.forename: Hafsa
authors.initials: H
authors.lastname: Madani
authors.forename: Hagai
authors.initials: H
authors.lastname: Yadin
authors.forename: Serife
authors.initials: S
authors.lastname: Incoglu
authors.forename: Kadir
authors.initials: K
authors.lastname: Yesilbag
authors.forename: Andrew B
authors.initials: AB
authors.lastname: Allison
authors.forename: David E
authors.initials: DE
authors.lastname: Stallknecht
authors.forename: Carrie
authors.initials: C
authors.lastname: Batten
authors.forename: Simon J
authors.initials: SJ
authors.lastname: Anthony
authors.forename: Peter P C
authors.initials: PP
authors.lastname: Mertens
mesh_terms: D000818:Animals; D036022:Capsid Proteins / Q000235:genetics; D002417:Cattle; D002418:Cattle Diseases / Q000821:virology*; D002460:Cell Line; D006224:Cricetinae; D018164:Hemorrhagic Disease Virus, Epizootic / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D019083:Mediterranean Region; D008969:Molecular Sequence Data; D009656:North America; D010802:Phylogeny; D012088:Reoviridae Infections / Q000662:veterinary* / Q000821:virology; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*
chemical_list: D036022:Capsid Proteins