Tetrazine-trans-cyclooctene ligation for the rapid construction of 18F labeled probes.
A radiolabeling method for bioconjugation based on the Diels-Alder reaction between 3,6-diaryl-s-tetrazines and an (18)F-labeled trans-cyclooctene is described. The reaction proceeds with exceptionally fast rates, making it an effective conjugation method within seconds at low micromolar concentrations.
Li
Zibo
Z
Molecular Imaging Center, Department of Radiology, University of Southern California, Los Angeles 90033, USA. ziboli@usc.edu
Cai
Hancheng
H
Hassink
Matthew
M
Blackman
Melissa L
ML
Brown
Richard C D
RC
Conti
Peter S
PS
Fox
Joseph M
JM
D002851:Chromatography, High Pressure Liquid; D034242:Cyclooctanes / Q000737:chemistry*; D005462:Fluorine Radioisotopes / Q000737:chemistry*; D011874:Radiometry
D034242:Cyclooctanes; D005462:Fluorine Radioisotopes