title: [Determination of arbutin in apple juice concentrate by ultra performance liquid chromatography with electrospray ionization tandem mass spectrometry].
abstract: An ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/ MS) method was developed for the determination of arbutin in apple juice concentrate. Samples were diluted with water, then cleaned-up with a PS-DVB column. Quantitation was carried out using an external standard method. UPLC was performed on an Eclipse Plus C, column (100 mm x 2.1 mm, 1.8 microm) using a gradient solvent system (methanol-water). MS/MS was performed with multiple reaction monitoring (MRM) mode. The detection limit of arbutin was 0.02 mg/L. The method showed good linear relationship at the range of 0.04-2.0 mg/L. The recoveries ranged from 75.2% to 102.7% with relative standard deviations (RSDs) less than 8.9%. The method is simple, fast and sensitive. It's suitable for quantitative and qualitative analysis of arbutin in apple juice concentrate.
authors.affiliation: Shaanxi Normal University, Xi' an 710062, China. kong-xianghong@163.com
authors.forename: Xianghong
authors.initials: X
authors.lastname: Kong
authors.forename: Qiang
authors.initials: Q
authors.lastname: He
authors.forename: Aishan
authors.initials: A
authors.lastname: Yue
authors.forename: Shuangmin
authors.initials: S
authors.lastname: Wu
authors.forename: Jianhua
authors.initials: J
authors.lastname: Li