title: Porous-CLEAs of papain: application to enzymatic hydrolysis of macromolecules.
abstract: Porous cross-linked enzyme aggregates (p-CLEAs) were prepared by adding starch as a pore-making agent, which facilitates CLEAs in cases where the substrates of enzyme are macromolecules. This novel strategy for preparation of p-CLEAs involves co-precipitation, cross-linking and removal of starch by α-amylase. The resulting papain p-CLEAs were characterized by scanning electron microscope (SEM) images, showing a porous structure. The 95.9% and 90.4% increased catalytic efficiencies of p-CLEAs than conventional CLEAs on bovine serum albumin (BSA) and ovalbumin verified the feasibility of this protocol.
authors.affiliation: State Key Laboratory of Chemical Engineering, Chemical Engineering Research Center, School of Chemical Engineering and Technology, Tianjin University, Tianjin, PR China.
authors.forename: Mengfan
authors.initials: M
authors.lastname: Wang
authors.forename: Chenxi
authors.initials: C
authors.lastname: Jia
authors.forename: Wei
authors.initials: W
authors.lastname: Qi
authors.forename: Qingxin
authors.initials: Q
authors.lastname: Yu
authors.forename: Xin
authors.initials: X
authors.lastname: Peng
authors.forename: Rongxin
authors.initials: R
authors.lastname: Su
authors.forename: Zhimin
authors.initials: Z
authors.lastname: He
mesh_terms: D004789:Enzyme Activation; D004800:Enzymes, Immobilized / Q000737:chemistry; D006868:Hydrolysis; D046911:Macromolecular Substances / Q000737:chemistry*; D010206:Papain / Q000737:chemistry*; D016062:Porosity; D013213:Starch / Q000737:chemistry*
chemical_list: D004800:Enzymes, Immobilized; D046911:Macromolecular Substances; D013213:Starch; D010206:Papain