Adrenomedullin attenuates aortic cross-clamping-induced myocardial injury in rats.
BACKGROUND
In this study we investigate the effects of adrenomedullin on myocardial injury after ischemia-reperfusion (I/R) after abdominal aortic surgery.


METHODS
Thirty-two Wistar rats were randomized into 4 groups (n = 8) as follows: control group (sham laparotomy), the aortic I/R group, aortic I/R plus adrenomedullin group (underwent aortic I/R periods, and received a bolus intravenous injection of .05 μg/kg/min adrenomedullin), and the control plus adrenomedullin group.


RESULTS
Biochemical analysis showed that aortic I/R significantly increased (P < .05) the plasma levels of troponin-I and tumor necrosis factor-α, and the myocardial tissue levels of malondialdehyde, superoxide dismutase, catalase, and angiotensin II, whereas aortic I/R plus adrenomedullin significantly decreased these same factors (P < .05). Aortic I/R significantly increased (P < .05) myocardial tissue levels of nitric oxide whereas aortic I/R plus adrenomedullin significantly increased the same factor (P < .05).


CONCLUSIONS
These results indicate that adrenomedullin has protective effects against myocardial injury induced by abdominal aortic I/R in rats.
Oyar
Eser Öz
EÖ
Department of Physiology, Gazi University, Ankara, Turkey. eserozoyar@yahoo.com
Kiriş
Ilker
I
Gülmen
Senol
S
Ceyhan
Betül Mermi
BM
Cüre
Medine Cumhur
MC
Sütcü
Recep
R
Lortlar
Neşe
N
Okutan
Hüseyin
H
D053607:Adrenomedullin / Q000097:blood / Q000378:metabolism*; D000804:Angiotensin II / Q000378:metabolism; D000818:Animals; D001011:Aorta; D017209:Apoptosis; D015415:Biomarkers / Q000378:metabolism; D053148:Caspase 3 / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D003250:Constriction; D019332:Endothelin-1 / Q000378:metabolism; D007150:Immunohistochemistry; D007249:Inflammation / Q000378:metabolism / Q000517:prevention & control; D015227:Lipid Peroxidation; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D015428:Myocardial Reperfusion Injury / Q000378:metabolism* / Q000473:pathology; D032383:Myocytes, Cardiac / Q000378:metabolism* / Q000473:pathology; D009569:Nitric Oxide / Q000378:metabolism; D018384:Oxidative Stress; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism; D019210:Troponin I / Q000097:blood; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
D015415:Biomarkers; D019332:Endothelin-1; D019210:Troponin I; D014409:Tumor Necrosis Factor-alpha; D000804:Angiotensin II; D053607:Adrenomedullin; D009569:Nitric Oxide; D008315:Malondialdehyde; D002374:Catalase; D013482:Superoxide Dismutase; D053148:Caspase 3