title: Benefits of blood group genotyping in multi-transfused patients from the south of Brazil.
abstract: We evaluated the usefulness of blood group genotyping as a supplement to hemagglutination to determine the red blood cell (RBC) antigen profile of polytransfused patients with hematological diseases and renal failure. Seventy-nine patients were selected. They all received more than three units of blood and eight (10%) had already clinical significant alloantibodies occurring alone or in combination against Rh, K, Fya, and Di antigens. DNA was prepared from blood samples and RHCE*E/e, KEL*01/KEL*02, FY*01/FY*02 and JK*01/JK*02 alleles were determined by using PCR-RFLP. RHD*/RHD*Ψ and RHCE*C/c were tested using multiplex PCR. Discrepancies for Rh, Kell, Duffy, and Kidd systems were found between the phenotype and genotype-derived phenotype in 16 of the 38 chronically transfused patients. The genotypes of these patients were confirmed by DNA array analysis (HEA Beadchip(™); Bioarray Solutions, Warren, NJ). Genotyping was very important for the determination of the true blood groups of the polytransfused patients, helped in the identification of suspected alloantibodies and in the selection of antigen-negative RBCs for transfusion.
authors.affiliation: Laboratório de Imunogenética, Departamento de Ciências Básicas da Saúde, Universidade Estadual de Maringá, Maringá, Brazil.
authors.forename: Gláucia Andréia Soares
authors.initials: GA
authors.lastname: Guelsin
authors.forename: Ana Maria
authors.initials: AM
authors.lastname: Sell
authors.forename: Lilian
authors.initials: L
authors.lastname: Castilho
authors.forename: Viviane Lika
authors.initials: VL
authors.lastname: Masaki
authors.forename: Fabiano Cavalcante
authors.initials: FC
authors.lastname: Melo
authors.forename: Margareth Naomi
authors.initials: MN
authors.lastname: Hashimoto
authors.forename: Tatiana Takahashi
authors.initials: TT
authors.lastname: Higa
authors.forename: Loide Souza
authors.initials: LS
authors.lastname: Hirle
authors.forename: Jeane Eliete Laguila
authors.initials: JE
authors.lastname: Visentainer
mesh_terms: D001789:Blood Group Antigens / Q000032:analysis / Q000235:genetics*; D001803:Blood Transfusion; D001938:Brazil; D016022:Case-Control Studies; D004247:DNA / Q000235:genetics*; D004375:Duffy Blood-Group System / Q000097:blood / Q000235:genetics*; D004912:Erythrocytes; D005838:Genotype; D006402:Hematologic Diseases / Q000097:blood / Q000235:genetics / Q000628:therapy; D006801:Humans; D007626:Kell Blood-Group System / Q000097:blood / Q000235:genetics*; D010641:Phenotype; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D011379:Prognosis; D051437:Renal Insufficiency / Q000097:blood / Q000235:genetics / Q000628:therapy; D012204:Rh-Hr Blood-Group System / Q000097:blood / Q000235:genetics*
chemical_list: D001789:Blood Group Antigens; D004375:Duffy Blood-Group System; D007626:Kell Blood-Group System; D012204:Rh-Hr Blood-Group System; D004247:DNA