title: Safety assessment of a wheat bran extract containing arabinoxylan-oligosaccharides: mutagenicity, clastogenicity, and 90-day rat-feeding studies.
abstract: Wheat bran extract (WBE) is a food-grade preparation that is highly enriched in arabinoxylan-oligosaccharides. As part of the safety evaluation of WBE, its genotoxic potential was assessed in a bacterial reverse mutagenicity assay (Ames test) and a chromosome aberration assay on Chinese hamster lung fibroblast cells. These in vitro genotoxicity assays showed no evidence of mutagenic or clastogenic activity with WBE. The safety of WBE was furthermore evaluated in a subchronic toxicity study on rats that were fed a semisynthetic diet (AIN 93G) containing 0.3%, 1.5%, or 7.5% WBE for 13 weeks, corresponding to an average intake of 0.2, 0.9, and 4.4 g/kg body weight (bw) per day, with control groups receiving the unsupplemented AIN 93G, AIN 93G with 7.5% inulin, or AIN 93G with 7.5% wheat bran. Based on this rat-feeding study, the no-observed-adverse-effect level (NOAEL) for WBE was determined as 4.4 g/kg (bw)/d, the highest dose tested.
authors.affiliation: Fugeia NV, Gaston Geenslaan 1, B-3001 Leuven, Belgium.
authors.forename: Isabelle E J A
authors.initials: IE
authors.lastname: François
authors.forename: Olivier
authors.initials: O
authors.lastname: Lescroart
authors.forename: Wim S
authors.initials: WS
authors.lastname: Veraverbeke
authors.forename: Raluca
authors.initials: R
authors.lastname: Kubaszky
authors.forename: Judit
authors.initials: J
authors.lastname: Hargitai
authors.forename: David J
authors.initials: DJ
authors.lastname: Esdaile
authors.forename: Erzsébet
authors.initials: E
authors.lastname: Beres
authors.forename: Madhu G
authors.initials: MG
authors.lastname: Soni
authors.forename: Andrew
authors.initials: A
authors.lastname: Cockburn
authors.forename: Willem F
authors.initials: WF
authors.lastname: Broekaert
mesh_terms: D000818:Animals; D001711:Biotransformation; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D004043:Dietary Fiber; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000187:drug effects; D005260:Female; D059022:Food Safety; D008297:Male; D009152:Mutagenicity Tests; D019055:No-Observed-Adverse-Effect Level; D009844:Oligosaccharides / Q000032:analysis*; D010936:Plant Extracts / Q000737:chemistry* / Q000378:metabolism / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D012486:Salmonella typhimurium / Q000187:drug effects; D012639:Seeds / Q000737:chemistry*; D018675:Toxicity Tests; D014908:Triticum / Q000737:chemistry*; D014990:Xylans / Q000032:analysis*
chemical_list: D004043:Dietary Fiber; D009844:Oligosaccharides; D010936:Plant Extracts; D014990:Xylans; C085118:arabinoxylan