Parental relationships among three grape varieties studied by MALDI of grape seed protein profiles.
Two Raboso cultivars, i.e. Raboso Veronese and Raboso Piave, are two black Vitis vinifera varieties. A genetic study suggested that Raboso Veronese is the progeny of a spontaneous cross between Raboso Piave and Marzemina Bianca cultivars. Parental relationships are usually investigated by genetic studies, which are effective to establish genetic links among different cultivars. Considering that proteome is the genome expression, in this article we evaluated the power of seed protein profiles obtained by matrix-assisted laser desorption/ionization (MALDI)/MS for parentage investigation. The three cultivars lead to very similar spectra with differences in the relative intensity of the most abundant species and the presence of very weak specific ions. In order to evaluate the analytical significance of these aspects, the variability due to instrumental factors and due to different harvesting areas and years of the same cultivars have been considered and measured by the calculation of discrepancy factor values. On one hand, the results obtained can be considered a valid confirmation of the genomic findings, whereas on the other hand, the results provide evidence for the ability of MALDI/MS to individuate minor differences in protein profiles of complex protein mixtures.
Bertazzo
Antonella
A
Dipartimento di Scienze Farmaceutiche, Universita' di Padova, Via Francesco Marzolo 5, 35131 Padova, Italy. antonella.bertazzo@unipd.it
De Rosso
Mirko
M
Dalla Vedova
Antonio
A
Agnolin
Fabio
F
Flamini
Riccardo
R
Traldi
Pietro
P
D015203:Reproducibility of Results; D055314:Seed Storage Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D012639:Seeds / Q000737:chemistry; D013045:Species Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D027843:Vitis / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
D055314:Seed Storage Proteins