Discrepancy of biologic behavior influenced by bone marrow derived cells in lung cancer.
Disseminated cancer cells may initially require local nutrients and growth factors to thrive and survive in bone marrow. However, data on the influence of bone marrow derived cells (BMDC, also called bone stromal cells in some publications) on lung cancer cells is largely unexplored. This study explored the mechanism of how bone stromal factors contribute to the bone tropism in lung cancer. The difference among lung cancer cell lines in their abilities to metastasize to bone was found using the SCID animal model. Supernatant of bone marrow aspiration (BM) and condition medium from human bone stromal cells (BSC) were used to study the activity of bone stromal factors. We found bone stromal factors significantly increased the proliferation, invasion, adhesion and expression of angiogenosis-related factors, and inhibited the apoptosis for high bone metastasis H460 lung cancer cells. These biologic effects were not seen in SPC-A1 or A549 cells, which are low bone metastasis lung cancer cells. Adhesion of H460 cells to surface coated with bone stromal cells can activate some signal transduction pathways, and alter the expression of adhesion associated factors, including integrin β 3 and ADAMTS-1, two potential targets related with bone metastasis. We concluded that bone marrow derived cells had a profound effect on biological behavior of lung cancers, therefore favoring the growth of lung cancer cells in bone.
Zhang
Jie
J
Department of Thoracic Surgery, Shanghai Cancer Hospital, Fudan University, Shanghai 200032, PR China.
Niu
Xiao-Min
XM
Liao
Mei-Lin
ML
Liu
Yun
Y
Sha
Hui-Fang
HF
Zhao
Yi
Y
Yu
Yong-Feng
YF
Tan
Qiang
Q
Xiang
Jia-Qing
JQ
Fang
Jing
J
Lv
Dan-Dan
DD
Li
Xue-Bing
XB
Lu
Shun
S
Chen
Hai-Quan
HQ
D000818:Animals; D001854:Bone Marrow Cells / Q000378:metabolism / Q000473:pathology*; D001859:Bone Neoplasms / Q000378:metabolism / Q000473:pathology* / Q000556:secondary; D045744:Cell Line, Tumor; D017077:Culture Media, Conditioned; D006801:Humans; D008175:Lung Neoplasms / Q000098:blood supply / Q000378:metabolism / Q000473:pathology* / Q000556:secondary; D051379:Mice; D016513:Mice, SCID; D009389:Neovascularization, Pathologic / Q000378:metabolism / Q000473:pathology; D017154:Stromal Cells / Q000378:metabolism / Q000473:pathology; D014407:Tumor Cells, Cultured
D017077:Culture Media, Conditioned