title: IL-10 polymorphism associated with decreased risk for mortality after burn injury.
abstract: OBJECTIVE
  Evaluation of single nucleotide polymorphisms (SNPs) in the interleukin-10 promoter (-592 and -819) on risk for death after burn injury.
  
  
  METHODS
  Association between the IL-10 SNPs and outcome after burn injury was evaluated in a cohort of 265 patients from Parkland Hospital, Dallas, TX with ≥ 15% TBSA burns without non-burn trauma (ISS ≤ 16), traumatic or anoxic brain injury or spinal cord injury, who survived >48 h under an IRB-approved protocol. Clinical data were collected prospectively and genotyping was conducted by TaqMan assay. Whole blood from 31 healthy volunteers was stimulated with LPS (100 ng/mL) to determine the level of IL-10 expression for each allele by enzyme-linked immunosorbent assay (ELISA).
  
  
  RESULTS
  After adjustment for percent total body surface area (TBSA) burned, inhalation injury, age, gender, and race/ethnicity, carriage of ‑592A and/or ‑819T was significantly associated (P = 0.014) with a decreased risk for death (adjusted odds ratio: 0.404; 95% CI: 0.197-0.829). As the candidate SNPs were in complete linkage disequilibrium, it was not possible to distinguish which allele was associated with decreased mortality risk. Age, inhalation injury, and full-thickness burn size were significantly associated with increased risk for death. In the LPS stimulated blood of healthy controls, carriage of the -592A and/or -819T allele demonstrated a trend for decreased levels of IL-10 (P = 0.079).
  
  
  CONCLUSIONS
  Carriage of the ‑592A and/or ‑819T allele in the IL-10 promoter appears to reduce the risk for death after burn injury.
authors.affiliation: Department of Surgery, The University of Texas Southwestern Medical Center, Dallas, Texas, USA.
authors.forename: Ryan M
authors.initials: RM
authors.lastname: Huebinger
authors.forename: Fernando
authors.initials: F
authors.lastname: Rivera-Chavez
authors.forename: Ling-Yu
authors.initials: LY
authors.lastname: Chang
authors.forename: Ming-Mei
authors.initials: MM
authors.lastname: Liu
authors.forename: Joseph P
authors.initials: JP
authors.lastname: Minei
authors.forename: Gary F
authors.initials: GF
authors.lastname: Purdue
authors.forename: John L
authors.initials: JL
authors.lastname: Hunt
authors.forename: Brett D
authors.initials: BD
authors.lastname: Arnoldo
authors.forename: Robert C
authors.initials: RC
authors.lastname: Barber
mesh_terms: D000328:Adult; D002056:Burns / Q000235:genetics* / Q000401:mortality*; D015331:Cohort Studies; D005260:Female; D005838:Genotype; D006801:Humans; D002534:Hypoxia, Brain / Q000401:mortality; D016753:Interleukin-10 / Q000235:genetics*; D015810:Linkage Disequilibrium; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic / Q000235:genetics; D012307:Risk Factors; D013119:Spinal Cord Injuries / Q000401:mortality; D055815:Young Adult
chemical_list: C508609:IL10 protein, human; D016753:Interleukin-10