A large indel mutation of the bovine ADD1/SREBP1c gene and its effects on growth traits in some native cattle breeds from China.
Adipocyte determination and differentiation-dependent factor 1/sterol regulatory element-binding protein-1c (ADD1/SREBP1c) is a major determinant of tissue differential lipogenic capacity in mammalian and avian species. The objectives of the present study were to focus on insertion-deletion polymorphism (indel) in the bovine ADD1/SREBP1c gene, and analyzing its effect on growth traits in a sample of 1035 cattle belonging to four Chinese cattle breeds. PCR-SSCP, DNA sequencing and agarose electrophoresis methods were used. The 778 bp PCR products of ADD1/SREBP1c gene exhibited three genotypes and two alleles were revealed: W and D. Frequencies of the W allele varied from 0.8651 to 1.000. The associations of the 84 bp indel mutation of ADD1/SREBP1c gene with growth traits of 265 Nanyang cows were analyzed. The animals with genotype WD had significantly higher birth weight, body weight, average daily gain than those with genotype WW at birth, 6-, 12-, 18-, and 24-month old (P < 0.05 or P < 0.01). These results suggest that the indel mutation of bovine ADD1/SREBP1c gene may influence the growth traits in cattle.
Huang
Yong-Zhen
YZ
College of Animal Science and Technology, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A&F University, No 22 Xinong Road, Yangling, 712100 Shaanxi, China. huangyongzhen2009@yahoo.com.cn
Zhang
En-Ping
EP
Wang
Jing
J
Huai
Yong-Tao
YT
Ma
Liang
L
Chen
Fu-Ying
FY
Lan
Xian-Yong
XY
Lei
Chu-Zhao
CZ
Fang
Xing-Tang
XT
Wang
Ju-Qiang
JQ
Chen
Hong
H
D000818:Animals; D020029:Base Pairing / Q000235:genetics; D001483:Base Sequence; D001947:Breeding; D002417:Cattle / Q000235:genetics* / Q000254:growth & development*; D002681:China; D004252:DNA Mutational Analysis; D004587:Electrophoresis, Agar Gel; D056726:Genetic Association Studies; D005828:Genetics, Population; D005838:Genotype; D054643:INDEL Mutation / Q000235:genetics*; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D019655:Quantitative Trait, Heritable; D051780:Sterol Regulatory Element Binding Protein 1 / Q000235:genetics*
D051780:Sterol Regulatory Element Binding Protein 1