Investigating the structural biofunctionality of antibodies conjugated to magnetic nanoparticles.
We present the synthesis of trastuzumab-functionalized pegylated iron oxide nanoparticles and provide an FTIR-based approach to gain a direct evidence of the actual conservation of the native structure of conjugated antibody. Their target-selectivity to specific cancer cell receptors has been also assessed.
Occhipinti
Emanuela
E
Dipartimento di Biotecnologie e Bioscienze, Università di Milano-Bicocca, P.za della Scienza 2, 20126, Milano, Italy.
Verderio
Paolo
P
Natalello
Antonino
A
Galbiati
Elisabetta
E
Colombo
Miriam
M
Mazzucchelli
Serena
S
Salvadè
Agnese
A
Tortora
Paolo
P
Doglia
Silvia Maria
SM
Prosperi
Davide
D
D000906:Antibodies / Q000737:chemistry*; D000911:Antibodies, Monoclonal / Q000737:chemistry; D061067:Antibodies, Monoclonal, Humanized; D001224:Aspartic Acid / Q000031:analogs & derivatives / Q000737:chemistry; D045744:Cell Line, Tumor; D005290:Ferric Compounds / Q000737:chemistry; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D006801:Humans; D018796:Immunoconjugates / Q000737:chemistry; D047468:Immunoprecipitation; D008280:Magnetics; D053768:Metal Nanoparticles / Q000737:chemistry*; D018613:Microscopy, Confocal; D009025:Morpholines / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D000068878:Trastuzumab
D000906:Antibodies; D000911:Antibodies, Monoclonal; D061067:Antibodies, Monoclonal, Humanized; D005290:Ferric Compounds; D018796:Immunoconjugates; D009025:Morpholines; C499728:poly (3-morpholinopropyl) aspartamide; C000499:ferric oxide; D001224:Aspartic Acid; D016650:Fluorescein-5-isothiocyanate; D000068878:Trastuzumab