title: Adiponectin concentrations: a genome-wide association study.
abstract: Adiponectin is associated with obesity and insulin resistance. To date, there has been no genome-wide association study (GWAS) of adiponectin levels in Asians. Here we present a GWAS of a cohort of Korean volunteers. A total of 4,001 subjects were genotyped by using a genome-wide marker panel in a two-stage design (979 subjects initially and 3,022 in a second stage). Another 2,304 subjects were used for follow-up replication studies with selected markers. In the discovery phase, the top SNP associated with mean log adiponectin was rs3865188 in CDH13 on chromosome 16 (p = 1.69 × 10(-15) in the initial sample, p = 6.58 × 10(-39) in the second genome-wide sample, and p = 2.12 × 10(-32) in the replication sample). The meta-analysis p value for rs3865188 in all 6,305 individuals was 2.82 × 10(-83). The association of rs3865188 with high-molecular-weight adiponectin (p = 7.36 × 10(-58)) was even stronger in the third sample. A reporter assay that evaluated the effects of a CDH13 promoter SNP in complete linkage disequilibrium with rs3865188 revealed that the major allele increased expression 2.2-fold. This study clearly shows that genetic variants in CDH13 influence adiponectin levels in Korean adults.
authors.affiliation: Department of Epidemiology and Health Promotion, Institute for Health Promotion, Graduate School of Public Health, Yonsei University, Seoul 102-752, South Korea. jsunha@yuhs.ac
authors.forename: Sun Ha
authors.initials: SH
authors.lastname: Jee
authors.forename: Jae Woong
authors.initials: JW
authors.lastname: Sull
authors.forename: Jong-Eun
authors.initials: JE
authors.lastname: Lee
authors.forename: Chol
authors.initials: C
authors.lastname: Shin
authors.forename: Jongkeun
authors.initials: J
authors.lastname: Park
authors.forename: Heejin
authors.initials: H
authors.lastname: Kimm
authors.forename: Eun-Young
authors.initials: EY
authors.lastname: Cho
authors.forename: Eun-Soon
authors.initials: ES
authors.lastname: Shin
authors.forename: Ji Eun
authors.initials: JE
authors.lastname: Yun
authors.forename: Ji Wan
authors.initials: JW
authors.lastname: Park
authors.forename: Sang Yeun
authors.initials: SY
authors.lastname: Kim
authors.forename: Sun Ju
authors.initials: SJ
authors.lastname: Lee
authors.forename: Eun Jung
authors.initials: EJ
authors.lastname: Jee
authors.forename: Inkyung
authors.initials: I
authors.lastname: Baik
authors.forename: Linda
authors.initials: L
authors.lastname: Kao
authors.forename: Sungjoo Kim
authors.initials: SK
authors.lastname: Yoon
authors.forename: Yangsoo
authors.initials: Y
authors.lastname: Jang
authors.forename: Terri H
authors.initials: TH
authors.lastname: Beaty
mesh_terms: D052242:Adiponectin / Q000097:blood*; D000328:Adult; D044466:Asian People / Q000235:genetics*; D001794:Blood Pressure; D015992:Body Mass Index; D015820:Cadherins / Q000235:genetics*; D002460:Cell Line; D002784:Cholesterol / Q000097:blood; D017931:DNA Primers / Q000235:genetics; D005260:Female; D055106:Genome-Wide Association Study; D005838:Genotype; D006801:Humans; D015810:Linkage Disequilibrium; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D052242:Adiponectin; D015820:Cadherins; D017931:DNA Primers; C099972:H-cadherin; D002784:Cholesterol