title: Cellulase activities in biomass conversion: measurement methods and comparison.
abstract: Cellulose, the major constituent of all plant materials and the most abundant organic molecule on the Earth, is a linear biopolymer of glucose molecules, connected by β-1,4-glycosidic bonds. Enzymatic hydrolysis of cellulose requires mixtures of hydrolytic enzymes including endoglucanases, exoglucanases (cellobiohydrolases), and β-glucosidases acting in a synergistic manner. In biopolymer hydrolysis studies, enzyme assay is an indispensable part. The most commonly used assays for the individual enzymes as well as total cellulase activity measurements, including their advantages and limitations, are summarized in this review article. In addition, some novel approaches recently used for enzyme assays are summarized.
authors.affiliation: Biorefining Research Initiative, Lakehead University, Thunder Bay, Ontario, Canada.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Dashtban
authors.forename: Miranda
authors.initials: M
authors.lastname: Maki
authors.forename: Kam Tin
authors.initials: KT
authors.lastname: Leung
authors.forename: Canquan
authors.initials: C
authors.lastname: Mao
authors.forename: Wensheng
authors.initials: W
authors.lastname: Qin
mesh_terms: D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D002480:Cellulase / Q000378:metabolism*; D057075:Enzyme Assays / Q000379:methods*; D001617:beta-Glucosidase / Q000378:metabolism
chemical_list: D001617:beta-Glucosidase; D002480:Cellulase