title: A new ultrafast and high-throughput mass spectrometric approach for the therapeutic drug monitoring of the multi-targeted anti-folate pemetrexed in plasma from lung cancer patients.
abstract: An analytical assay has been developed and validated for ultrafast and high-throughput mass spectrometric determination of pemetrexed concentrations in plasma using matrix assisted laser desorption/ionization-triple quadrupole-tandem mass spectrometry. Patient plasma samples spiked with the internal standard methotrexate were measured by multiple reaction monitoring. The detection limit was 0.4 fmol/μL, lower limit of quantification was 0.9 fmol/μL, and upper limit of quantification was 60 fmol/μL, respectively. Overall observed pemetrexed concentrations in patient samples ranged between 8.7 (1.4) and 142.7 (20.3) pmol/μL (SD). The newly developed mass spectrometric assay is applicable for (routine) therapeutic drug monitoring of pemetrexed concentrations in plasma from non-small cell lung cancer patients.
authors.affiliation: Department of Neurology, Laboratory of Neuro-Oncology and Clinical & Cancer Proteomics, University Medical Center Rotterdam (ErasmusMC), Dr. Molewaterplein 50, Room Ee-1981, 3015 GE Rotterdam, The Netherlands. r.meesters@erasmusmc.nl
authors.forename: Roland J W
authors.initials: RJ
authors.lastname: Meesters
authors.forename: Robin
authors.initials: R
authors.lastname: Cornelissen
authors.forename: Rob J
authors.initials: RJ
authors.lastname: van Klaveren
authors.forename: Robert
authors.initials: R
authors.lastname: de Jonge
authors.forename: Ethan
authors.initials: E
authors.lastname: den Boer
authors.forename: Jan
authors.initials: J
authors.lastname: Lindemans
authors.forename: Theo M
authors.initials: TM
authors.lastname: Luider
mesh_terms: D000964:Antimetabolites, Antineoplastic / Q000097:blood* / Q000627:therapeutic use; D002289:Carcinoma, Non-Small-Cell Lung / Q000097:blood* / Q000188:drug therapy; D016903:Drug Monitoring / Q000379:methods; D005971:Glutamates / Q000097:blood* / Q000627:therapeutic use; D006147:Guanine / Q000031:analogs & derivatives* / Q000097:blood / Q000627:therapeutic use; D006801:Humans; D008175:Lung Neoplasms / Q000097:blood* / Q000188:drug therapy; D000068437:Pemetrexed; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000964:Antimetabolites, Antineoplastic; D005971:Glutamates; D000068437:Pemetrexed; D006147:Guanine