title: Comparisons of DNA marker-based genetic diversity with phenotypic estimates in maize grown in Pakistan.
abstract: We compared DNA-based genetic diversity estimates with conventional estimates by investigating agronomically important traits in maize grown in the northwestern region of Pakistan. RAPD markers were used to characterize 10 commonly cultivated maize genotypes. The same material was tested for phenotypic variation of quantitative traits using replicated field trials. The genetic distances between pairs of genotypes using RAPD data were used to generate a similarity matrix and to construct a phenogram. Statistical analyses were carried out on the data obtained from field trials of all maize genotypes for days to 50% tasseling, days to 50% silking, plant height, ear height, grain yield, grain weight per cob, and ear length. Analysis of variance and single degree of freedom contrasts were performed on morphological data to examine the relationship between molecular-based clusters and agronomic traits. A molecular marker-based phenogram led to the grouping of all genotypes into four major clusters, some of which were distantly related. These clusters contained one to four genotypes. Analysis of variance showed significant variations among all genotypes for agronomic traits. The single degree of freedom contrasts between groups of genotypes indicated significant differences for most traits. Pair-wise comparisons between clusters were also significant. The two types of data correlated well, providing an opportunity for better choices for selection.
authors.affiliation: Biotechnology Program, Department of Environmental Sciences, Commission on Science and Technology for Sustainable Development in the South, Institute of Information Technology, University Road, Abbottabad, Pakistan. mmshah@ciit.net.pk
authors.forename: M M
authors.initials: MM
authors.lastname: Shah
authors.forename: S W
authors.initials: SW
authors.lastname: Hassan
authors.forename: K
authors.initials: K
authors.lastname: Maqbool
authors.forename: I
authors.initials: I
authors.lastname: Shahzadi
authors.forename: A
authors.initials: A
authors.lastname: Pervez
mesh_terms: D005819:Genetic Markers / Q000235:genetics*; D014644:Genetic Variation / Q000235:genetics; D005838:Genotype; D010154:Pakistan; D019105:Random Amplified Polymorphic DNA Technique; D003313:Zea mays / Q000235:genetics*
chemical_list: D005819:Genetic Markers