title: Bioelectrochemical control of neural cell development on conducting polymers.
abstract: Electrically conducting polymers hold promise for developing advanced neuroprostheses, bionic systems and neural repair devices. Among them, poly(3, 4-ethylenedioxythiophene) doped with polystyrene sulfonate (PEDOT:PSS) exhibits superior physicochemical properties but biocompatibility issues have limited its use. We describe combinations of electrochemical and molecule self-assembling methods to consistently control neural cell development on PEDOT:PSS while maintaining very low interfacial impedance. Electro-adsorbed polylysine enabled long-term neuronal survival and growth on the nanostructured polymer. Neurite extension was strongly inhibited by an additional layer of PSS or heparin, which in turn could be either removed electrically or further coated with spermine to activate cell growth. Binding basic fibroblast growth factor (bFGF) to the heparin layer inhibited neurons but promoted proliferation and migration of precursor cells. This methodology may orchestrate neural cell behavior on electroactive polymers, thus improving cell/electrode communication in prosthetic devices and providing a platform for tissue repair strategies.
authors.affiliation: Neural Repair Laboratory, Hospital Nacional de Parapléjicos, Finca La Peraleda s/n, Toledo, Spain. jcollazos@sescam.org
authors.forename: Jorge E
authors.initials: JE
authors.lastname: Collazos-Castro
authors.forename: José L
authors.initials: JL
authors.lastname: Polo
authors.forename: Gabriel R
authors.initials: GR
authors.lastname: Hernández-Labrado
authors.forename: Vanesa
authors.initials: V
authors.lastname: Padial-Cañete
authors.forename: Concepción
authors.initials: C
authors.lastname: García-Rama
mesh_terms: D000818:Animals; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000737:chemistry / Q000494:pharmacology; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004563:Electrochemistry; D016222:Fibroblast Growth Factor 2 / Q000737:chemistry / Q000494:pharmacology; D006493:Heparin / Q000737:chemistry / Q000494:pharmacology; D008854:Microscopy, Electron; D009474:Neurons / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D011108:Polymers / Q000737:chemistry* / Q000494:pharmacology; D011137:Polystyrenes / Q000737:chemistry / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D013096:Spermine / Q000737:chemistry / Q000494:pharmacology
chemical_list: D019086:Bridged Bicyclo Compounds, Heterocyclic; D011108:Polymers; D011137:Polystyrenes; C121383:poly(3,4-ethylene dioxythiophene); D016222:Fibroblast Growth Factor 2; D013096:Spermine; C003321:polystyrene sulfonic acid; D006493:Heparin