title: The porcine MUC20 gene: molecular characterization and its association with susceptibility to enterotoxigenic Escherichia coli F4ab/ac.
abstract: Enterotoxigenic Eschoerchia coli (ETEC) F4 is a major cause of diarrhea in neonatal and young pigs. The locus encoding intestinal receptor for ETEC F4ab/ac has been mapped to pig chromosome (SSC) 13q41. We previously assigned mucin 20 (MUC20) to SSC13q41 and considered it a candidate for the ETEC F4ab/ac receptor. In this study, the full-length mRNA sequence of the porcine MUC20 gene was determined, revealing two MUC20 alternative transcripts differing in an 18-bp deletion in the open reading frame (ORF). The longer isoform is 1,927-bp long and contains a 1,572-bp ORF encoding a protein of 523 amino acids. RT-PCR analysis showed that MUC20 mRNA was expressed most highly in the kidney, prostate, epididymis and bladder. Seven single nucleotide polymorphisms in MUC20 were identified in founder animals of a White Duroc × Erhualian cross, and the entire cross population (n = 842) was genotyped for two MUC20 polymorphisms: g.191C>T in intron 5 and c.1600C>T in exon 6. The transmission disequilibrium test (TDT) results revealed that both polymorphisms were significantly associated with in vitro ETEC F4ab/ac adhesion phenotypes in the intercross population, and the most significant association was found between g.191C>T and F4ac adhesion phenotype (P = 1.02 × 10(-9)).
authors.affiliation: Key Laboratory for Animal Biotechnology of Jiangxi Province and the Ministry of Agriculture of China, Jiangxi Agricultural University, 330045 Nanchang, China.
authors.forename: Huayuan
authors.initials: H
authors.lastname: Ji
authors.forename: Jun
authors.initials: J
authors.lastname: Ren
authors.forename: Xueming
authors.initials: X
authors.lastname: Yan
authors.forename: Xiang
authors.initials: X
authors.lastname: Huang
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
authors.forename: Zhiyan
authors.initials: Z
authors.lastname: Zhang
authors.forename: Lusheng
authors.initials: L
authors.lastname: Huang
mesh_terms: D000483:Alleles; D017398:Alternative Splicing / Q000235:genetics; D000818:Animals; D003433:Crosses, Genetic; D018076:DNA, Complementary / Q000235:genetics; D054307:Enterotoxigenic Escherichia coli / Q000502:physiology*; D004927:Escherichia coli Infections / Q000235:genetics / Q000662:veterinary*; D005260:Female; D020869:Gene Expression Profiling; D020022:Genetic Predisposition to Disease; D006239:Haplotypes / Q000235:genetics; D015810:Linkage Disequilibrium / Q000235:genetics; D008297:Male; D008969:Molecular Sequence Data; D009077:Mucins / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D034421:Sus scrofa / Q000235:genetics*; D013552:Swine; D013553:Swine Diseases / Q000235:genetics* / Q000382:microbiology*
chemical_list: D018076:DNA, Complementary; D009077:Mucins; D012333:RNA, Messenger