Domain altering SNPs in the human proteome and their impact on signaling pathways.
Single nucleotide polymorphisms (SNPs) constitute an important mode of genetic variations observed in the human genome. A small fraction of SNPs, about four thousand out of the ten million, has been associated with genetic disorders and complex diseases. The present study focuses on SNPs that fall on protein domains, 3D structures that facilitate connectivity of proteins in cell signaling and metabolic pathways. We scanned the human proteome using the PROSITE web tool and identified proteins with SNP containing domains. We showed that SNPs that fall on protein domains are highly statistically enriched among SNPs linked to hereditary disorders and complex diseases. Proteins whose domains are dramatically altered by the presence of an SNP are even more likely to be present among proteins linked to hereditary disorders. Proteins with domain-altering SNPs comprise highly connected nodes in cellular pathways such as the focal adhesion, the axon guidance pathway and the autoimmune disease pathways. Statistical enrichment of domain/motif signatures in interacting protein pairs indicates extensive loss of connectivity of cell signaling pathways due to domain-altering SNPs, potentially leading to hereditary disorders.
Liu
Yichuan
Y
Center for Integrated Bioinformatics, Drexel University, Philadelphia, Pennsylvania, United States of America.
Tozeren
Aydin
A
D030562:Databases, Protein; D030342:Genetic Diseases, Inborn / Q000235:genetics / Q000378:metabolism; D015894:Genome, Human; D006801:Humans; D020641:Polymorphism, Single Nucleotide; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry* / Q000235:genetics*; D020543:Proteome / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction
D011506:Proteins; D020543:Proteome