title: An ancient P-loop GTPase in rice is regulated by a higher plant-specific regulatory protein.
abstract: YchF is a subfamily of the Obg family in the TRAFAC class of P-loop GTPases. The wide distribution of YchF homologues in both eukarya and bacteria suggests that they are descendents of an ancient protein, yet their physiological roles remain unclear. Using the OsYchF1-OsGAP1 pair from rice as the prototype, we provide evidence for the regulation of GTPase/ATPase activities and RNA binding capacity of a plant YchF (OsYchF1) by its regulatory protein (OsGAP1). The effects of OsGAP1 on the subcellular localization/cycling and physiological functions of OsYchF1 are also discussed. The finding that OsYchF1 and OsGAP1 are involved in plant defense response might shed light on the functional roles of YchF homologues in plants. This work suggests that during evolution, an ancestral P-loop GTPase/ATPase may acquire new regulation and function(s) by the evolution of a lineage-specific regulatory protein.
authors.affiliation: State Key Laboratory of Agrobiotechnology and School of Life Sciences, Chinese University of Hong Kong, Shatin, Hong Kong.
authors.forename: Ming-Yan
authors.initials: MY
authors.lastname: Cheung
authors.forename: Yan
authors.initials: Y
authors.lastname: Xue
authors.forename: Liang
authors.initials: L
authors.lastname: Zhou
authors.forename: Man-Wah
authors.initials: MW
authors.lastname: Li
authors.forename: Samuel Sai-Ming
authors.initials: SS
authors.lastname: Sun
authors.forename: Hon-Ming
authors.initials: HM
authors.lastname: Lam
mesh_terms: D019143:Evolution, Molecular; D020558:GTP Phosphohydrolases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020690:GTPase-Activating Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D012275:Oryza / Q000737:chemistry / Q000145:classification / Q000201:enzymology* / Q000235:genetics; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D013045:Species Specificity
chemical_list: D020690:GTPase-Activating Proteins; D010940:Plant Proteins; D020558:GTP Phosphohydrolases