[Construction of the genetically attenuated bacteria Bordetella pertussis devoid of dermonecrotic toxin activity and producing modified nontoxic pertussis toxin form].
The recombinant modified (attenuated) bacteria A. pertussis were constructed. These bacteria contained knockout mutation of the dnt gene and produced nontoxic pertussis toxin derivative. The immunological properties of the mutant bacteria B. pertussis strain KS were studied. The recombinant bacteria B. pertussis strain KS were found to be devoid of dermonecrotic toxin activity, conserved the structure of the mutant dnt gene in condition of cultivation on selective growth media, and long-term survival in laboratory animal organism. Intranasal immunization of mice with living bacteria B. pertussis, attenuated strain KS provided protection of animals from virulent strains of the pertussis. The efficiency of the protection was comparable with protection efficiency provided by standard corpuscular pertussis vaccine OSO-3.
Siniashina
L N
LN
Siniashina
L S
LS
Semin
E G
EG
Amelina
I P
IP
Karataev
G I
GI
D000281:Administration, Intranasal; D000818:Animals; D001886:Bordetella pertussis / Q000235:genetics* / Q000276:immunology; D005612:Freeze Drying; D020868:Gene Silencing; D006168:Guinea Pigs; D007928:Lethal Dose 50; D051379:Mice; D009154:Mutation; D010567:Pertussis Vaccine / Q000008:administration & dosage / Q000235:genetics* / Q000276:immunology; D011503:Transglutaminases / Q000235:genetics*; D014613:Vaccines, Attenuated / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D010566:Virulence Factors, Bordetella / Q000235:genetics*; D014917:Whooping Cough / Q000517:prevention & control*
D010567:Pertussis Vaccine; D014613:Vaccines, Attenuated; D014614:Vaccines, Synthetic; D010566:Virulence Factors, Bordetella; C060969:dermonecrotic toxin, Bordetella; D011503:Transglutaminases