title: Origin and molecular organization of supernumerary chromosomes of Prochilodus lineatus (characiformes, prochilodontidae) obtained by DNA probes.
abstract: In Prochilodus lineatus B-chromosomes are visualized as reduced size extra elements identified as microchromosomes and are variable in morphology and number. We describe the specific total probe (B-chromosome probe) in P. lineatus obtained by chromosome microdissection and a whole genomic probe (genomic probe) from an individual without B-chromosome. The specific B-chromosome was scraped and processed to obtain DNA with amplification by DOP-PCR, and so did the genomic probe DNA. Fluorescence in situ hybridization using the B-chromosome probe labeled with dUTP-Tetramethyl-rhodamine and the genomic probe labeled with digoxigenin-FITC permitted to establish that in this species supernumerary chromosomes with varying number and morphology had different structure of chromatin when compared to that of the regular chromosomes or A complement, since only these extra elements were labeled in the metaphases. The present findings suggest that modifications in the chromatin structure of B-chromosomes to differentiate them from the A chromosomes could occur along their dispersion in the individuals of the population.
authors.affiliation: Laboratório de Genética de Peixes, Departamento Ciências Biológicas, Faculdade de Ciências, Universidade Estadual Paulista (UNESP), Campus de Bauru, Av. Engenheiro Luiz Edmundo C. Coube, s/n, 17033-360 Bauru, SP, Brazil.
authors.forename: Tatiana Aparecida
authors.initials: TA
authors.lastname: Voltolin
authors.forename: Alejandro
authors.initials: A
authors.lastname: Laudicina
authors.forename: José Augusto
authors.initials: JA
authors.lastname: Senhorini
authors.forename: Jehud
authors.initials: J
authors.lastname: Bortolozzi
authors.forename: Cláudio
authors.initials: C
authors.lastname: Oliveira
authors.forename: Fausto
authors.initials: F
authors.lastname: Foresti
authors.forename: Fábio
authors.initials: F
authors.lastname: Porto-Foresti
mesh_terms: D000818:Animals; D002843:Chromatin; D002875:Chromosomes; D020732:Cytogenetic Analysis; D015342:DNA Probes / Q000235:genetics*; D019143:Evolution, Molecular; D005260:Female; D005399:Fishes / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D008297:Male
chemical_list: D002843:Chromatin; D015342:DNA Probes