title: [Study of bone marrow stroma stem cells cell-sheet and endothelial progenitor cells cultured in vitro on allogenic demineralized bone matrix in mongrel].
abstract: OBJECTIVE
  To investigate the growth situation of bone marrow stroma stem cells(BMSCs) cell-sheet and endothelial progenitor cells (EPCs) on demineralized bone matrix (DBM) and explore the effect of DBM/BMSCs cell-sheet and DBM/EPCs complex on the construction of functional and vascularized tissue-engineered bone in mongrel.
  
  
  METHODS
  BMSC cell-sheet was prepared with cell-sheet engineering approach, EPCs were isolated from canine bone marrow and DBM was prepared from homologous bone. BMSCs cell-sheet and EPCs were inoculated on DBM respectively and observed by inverted phase contrast microscope and scanning electron microscope. The porosity of DBM was measured.
  
  
  RESULTS
  The DBM/BMSCs cell-sheet and DBM/EPCs complex were successfully constructed. The BMSCs cell-sheet and EPCs adhered to DBM well and grew rapidly.
  
  
  CONCLUSIONS
  The BMSCs cell-sheet and EPCs have good biocompatibility with DBM. The complex of DBM/ BMSCs cell-sheet, DBM/EPCs could provide good conditions to acquire functional and vascularized tissue-engineered bone. Supported by National Natural Science Foundation of China (Grant No.30872896) and Natural Science Foundation of Shandong Province (Grant No.Y2008C77).
authors.affiliation: Department of Oral and Maxillofacial Surgery, Affiliated Hospital of Medical College, Qingdao University, Qingdao 266003, Shandong Province, China. tan_shuai@hotmail.com
authors.forename: Shuai
authors.initials: S
authors.lastname: Tan
authors.forename: Ning-yi
authors.initials: NY
authors.lastname: Li
authors.forename: Heng
authors.initials: H
authors.lastname: Jing
authors.forename: Zhen-hua
authors.initials: ZH
authors.lastname: Gao
authors.forename: Li-qiang
authors.initials: LQ
authors.lastname: Chen
mesh_terms: D001853:Bone Marrow; D001854:Bone Marrow Cells; D001857:Bone Matrix; D001842:Bone and Bones; D066026:Endothelial Progenitor Cells; D006801:Humans; D066298:In Vitro Techniques; D059630:Mesenchymal Stem Cells; D010012:Osteogenesis; D023822:Tissue Engineering; D054457:Tissue Scaffolds