The Escherichia coli CRISPR system protects from λ lysogenization, lysogens, and prophage induction.
We show that phage lysogenization, lysogens, and prophage induction are all targeted by CRISPR. The results demonstrate that genomic DNA is not immune to the CRISPR system, that the CRISPR system does not require noncytoplasmic elements, and that the system protects from phages entering and exiting the lysogenic cycle.
Edgar
Rotem
R
Department of Clinical Microbiology and Immunology, Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel.
Qimron
Udi
U
D010582:Bacteriophage lambda / Q000254:growth & development / Q000502:physiology*; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000821:virology*; D008242:Lysogeny; D012091:Repetitive Sequences, Nucleic Acid; D014775:Virus Activation; D016662:Virus Integration
D004269:DNA, Bacterial