[Expression of iNOS and HIF-1α with angiogenesis in affected skin biopsies from patients with psoriasis].
OBJECTIVE
To investigate the expression of inducible nitric oxide synthase (iNOS) and hypoxia-inducible factor 1(HIF-1) α in psoriatic lesions,and to explore their relationship with angiogenesis in psoriasis.


METHODS
HIF-1α and iNOS protein were detected by immunohistochemistry and Western blot in 32 cases of psoriasis and 20 healthy controls,and CD34 marking vascular endothelial cells were used to measure the microvascular density (MVD).


RESULTS
The expressions of HIF-1α and iNOS protein were very weak in the control skin, but very strong in psoriatic lesions, which showed significant difference in the expressions of iNOS and HIF-1α between the psoriasis and the control group(P<0.05). Expression of HIF-1α (r=0.743, P<0.01) and iNOS (r=0.639,P<0.01) had positive correlation with MVD in psoriasis respectively. There was a positive correlation between iNOS and HIF-1α expression in psoriasis (r=0.717, P<0.01).


CONCLUSIONS
Both iNOS and HIF-1α have high expression in psoriasis and might play an important role in the genesis and development of psoriasis.
Li
Yongjian
Y
Department of Dermatology, The First Affiliated Hospital of Nanhua University, Hengyang Hunan, China.
Zhang
Guiying
G
Xiao
Rong
R
Chen
Huan
H
Wen
Haiquan
H
D000293:Adolescent; D000328:Adult; D001706:Biopsy; D002648:Child; D005260:Female; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009389:Neovascularization, Pathologic; D052247:Nitric Oxide Synthase Type II / Q000235:genetics / Q000378:metabolism*; D011565:Psoriasis / Q000378:metabolism* / Q000473:pathology; D012867:Skin / Q000098:blood supply* / Q000378:metabolism / Q000473:pathology; D055815:Young Adult
C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; C496319:NOS2 protein, human; D052247:Nitric Oxide Synthase Type II