Potentiation of EBV-induced B Cell transformation by CXCR4-tropic, but not CCR5-tropic, HIV gp120: implications for HIV-associated lymphomagenesis.
Abstract R5 and X4 HIV strains use CCR5 or CXCR4 chemokine receptors (CKRs), respectively, for entry. Preferential growth of X4 vs. R5 HIV in cell lines reflects constitutive expression of CXCR4, but not CCR5 (in contrast to dual expression on primary T cells), and CXCR4 is the predominant CKR found on most tumors. Non-Hodgkin's B cell lymphomas (NHL) are increased among HIV(+) patients, and interactions between HIV envelope and CKRs may contribute to lymphomagenesis. Despite strong evidence for a CXCR4-SDF-1 oncogenic axis, no in vitro evaluation of CXCR4-mediated normal lymphocyte transformation has been published. Exposure of normal B cells to EBV in the presence of X4 gp120 (but not R5 gp120) increased proliferation and BLCL outgrowth, comparable to anti-CD40 mAb costimulation. This suggests a role for X4 tropic viral envelope signaling via CXCR4 and/or CXCR7 in HIV-associated lymphomagenesis.
Iyengar
Sujatha
S
Department of Molecular Microbiology and Immunology, The Bloomberg School of Public Health, Johns Hopkins University , Baltimore, Maryland.
Schwartz
David H
DH
D000328:Adult; D001402:B-Lymphocytes / Q000502:physiology / Q000821:virology*; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D006678:HIV / Q000472:pathogenicity*; D015699:HIV Envelope Protein gp120 / Q000378:metabolism*; D004854:Herpesvirus 4, Human / Q000472:pathogenicity*; D006801:Humans; D056189:Viral Tropism
D015699:HIV Envelope Protein gp120