Isolation and characterization of Reyranella massiliensis gen. nov., sp. nov. from freshwater samples by using an amoeba co-culture procedure.
The analysis of three water samples from two cooling towers and one river allowed us to isolate three strains of a novel species of the class Alphaproteobacteria which is phylogenetically related to uncultured alphaproteobacteria. Based upon 16S rRNA gene sequence analysis and phenotypic characterization, we propose to name this novel species Reyranella massiliensis gen. nov., sp. nov., type strain 521(T) ( = CSUR P115(T)  = DSM 23428(T)). The most closely related cultivable micro-organism to this novel bacterium is a member of the genus Magnetospirillum.
Pagnier
Isabelle
I
URMITE, CNRS UMR 6236, Faculté de Médecine de Marseille, 13385 Marseille Cedex 05, France.
Raoult
Didier
D
URMITE, CNRS UMR 6236, Faculté de Médecine de Marseille, 13385 Marseille Cedex 05, France.
La Scola
Bernard
B
URMITE, CNRS UMR 6236, Faculté de Médecine de Marseille, 13385 Marseille Cedex 05, France.
D000656:Amoeba / Q000382:microbiology*; D015373:Bacterial Typing Techniques; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005618:Fresh Water / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012245:Rhodospirillales / Q000145:classification / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification*; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S