Full-field time-resolved fluorescence tomography of small animals.
In this experimental investigation, we explore the feasibility of using wide-field illumination for time-resolved fluorescence molecular tomography. The performance of wide-field patterns with a time-resolved imaging platform is investigated in vitro and in a small animal model. A Monte Carlo-based forward model is employed to reconstruct fluorescence yield based on time-gated datasets. An improvement in resolution and quantification when using the time-gate data type compared to the commonly used cw data type is demonstrated in vitro. Furthermore, the feasibility of wide-field strategies for fluorescence preclinical applications is established by an accurate localization of a fluorescent inclusion implanted in the chest cavity of a murine model.
Venugopal
Vivek
V
Department of Biomedical Engineering, Rensselaer Polytechnic Institute, Troy, New York 12180, USA.
Chen
Jin
J
Lesage
Frederic
F
Intes
Xavier
X
D000818:Animals; D005240:Feasibility Studies; D021621:Imaging, Three-Dimensional; D051379:Mice; D009010:Monte Carlo Method; D019047:Phantoms, Imaging; D013050:Spectrometry, Fluorescence / Q000379:methods*; D014054:Tomography / Q000379:methods*