NaCl pretreatment alleviates salt stress by enhancement of antioxidant defense system and osmolyte accumulation in mungbean (Vigna radiata L. Wilczek).
Enhancement of salt (NaCl) tolerance by pretreatment with sublethal dose (50 mM) of NaCl was investigated in V. radiata seedlings. NaCl stress caused drastic effects on roots compared to shoots. Accompanying reductions in length, number of root hairs and branches, roots became stout, brittle and brown in color. Salt stress caused gradual reduction in chlorophyll, carotenoid pigment contents and chlorophyll fluorescence intensity also. Superoxide dismutase and catechol peroxidase activities increased under stress in both roots and leaves. But catalase activity showed an increase in roots and decrease in leaves. In these seedlings, the oxidative stress has been observed under salinity stress and the level of proline, H2O2 and malondialdehyde content were increased. But pretreatment with sublethal dose of NaCl was able to overcome the adverse effects of stress imposed by NaCl to variable extents by increasing growth and photosynthetic pigments of the seedlings, modifying the activities of antioxidant enzymes, reducing malondialdehyde and H2O2 content and increasing accumulation of osmolytes like proline. Thus, mungbean plants can acclimate to lethal level of salinity by pretreatment with sublethal level of NaCl, improving their health and production under saline condition.
Saha
Papiya
P
Plant Physiology and Biochemistry Laboratory, Department of Botany, University of Calcutta 35, Ballygunge Circular Road, Kolkata 700 019, India.
Chatterjee
Paramita
P
Biswas
Asok K
AK
D000975:Antioxidants / Q000378:metabolism*; D002338:Carotenoids / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D002734:Chlorophyll / Q000378:metabolism; D007887:Fabaceae / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008315:Malondialdehyde / Q000378:metabolism; D009994:Osmolar Concentration; D018384:Oxidative Stress / Q000187:drug effects*; D010544:Peroxidases / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D018520:Plant Shoots / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D054712:Salinity; D036226:Seedlings / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism
D000975:Antioxidants; D002734:Chlorophyll; D002338:Carotenoids; D012965:Sodium Chloride; D008315:Malondialdehyde; D006861:Hydrogen Peroxide; D010544:Peroxidases; C019850:catechol peroxidase; D002374:Catalase; D013482:Superoxide Dismutase