title: Construction of tissue-engineered corpus cavernosum with muscle-derived stem cells and transplantation in vivo.
abstract: OBJECTIVE
  • To investigate the feasibility of tissue-engineered corpus cavernosum (TECC) with muscle-derived stem cells (MDSCs) as seed cells and determine the growth potential in vivo.
  
  
  METHODS
  • Acellular corporal collagen matrices (ACCMs) were obtained from adult rabbit penis by a cell removal procedure. MDSCs were separated and purified using a digestion method and Preplate technique, then seeded on ACCMs at a concentration of 30 × 10(6) cells/mL to construct TECCs. After 5 days of culture, seeded ACCMs were implanted with albuginea of rabbits. The implants were retrieved at 2, 4 and 6 months after implantation. • Histochemistry, immunohistochemisry and scanning electron microscopy were performed to analyse the morphological characteristics of the TECCs.
  
  
  RESULTS
  • The decellularization process successfully extracted all cellular components while preserving the original collagen fibres. • Histological analyses of the explants at all time points in the experimental group had more cells and better arranged growth than the control group. α-Smooth muscle actin and endothelial nitric oxide synthase-positive cells were more prevalent in the experimental group.
  
  
  CONCLUSIONS
  • Our study showed that MDSCs can be seeded on three-dimensional ACCM scaffolds and develop tissues that are similar to native normal corpus cavernosum.
authors.affiliation: Department of Plastic Surgery, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Zhengzhou, China.
authors.forename: Chenyang
authors.initials: C
authors.lastname: Ji
authors.forename: Fuli
authors.initials: F
authors.lastname: Min
authors.forename: Weiqiang
authors.initials: W
authors.lastname: Liang
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Chen
authors.forename: Shujuan
authors.initials: S
authors.lastname: Pan
authors.forename: Lianjie
authors.initials: L
authors.lastname: Bin
authors.forename: Shihong
authors.initials: S
authors.lastname: Chen
authors.forename: Zhe
authors.initials: Z
authors.lastname: Wei
authors.forename: Tao
authors.initials: T
authors.lastname: He
authors.forename: Yanhui
authors.initials: Y
authors.lastname: Gu
authors.forename: Jinming
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002478:Cells, Cultured; D005109:Extracellular Matrix; D005240:Feasibility Studies; D005434:Flow Cytometry; D007150:Immunohistochemistry; D008297:Male; D032342:Muscle Cells / Q000166:cytology*; D009130:Muscle, Smooth / Q000166:cytology; D010413:Penis / Q000166:cytology* / Q000601:surgery; D011817:Rabbits; D013234:Stem Cells / Q000166:cytology*; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds