title: Inhibition of Vibrio cholerae biofilm by AiiA enzyme produced from Bacillus spp.
abstract: Vibrio cholerae is the causative agent of water-borne diarrheal disease, cholera. The formation of biofilm favors survival and persistence of V. cholerae in the aquatic environment and also inside the host. AHL lactonase (AiiA), a metallo-beta-lactamase produced by Bacillus spp., blocks quorum sensing in Gram-negative bacteria by hydrolyzing N-acyl-homoserine lactones (AHLs). In the present investigation, AiiA-mediated inhibition of V. cholerae biofilm was studied. Two novel alleles of aiiA-encoding genes from Bacillus spp. were expressed in E. coli, and the results demonstrated that AiiA enzyme is a potent inhibitor of V. cholerae biofilm.
authors.affiliation: Cholera and Environmental Microbiology Lab, Department of Molecular Microbiology, Rajiv Gandhi Centre for Biotechnology, Trivandrum, Kerala 695014, India.
authors.forename: Nimmy
authors.initials: N
authors.lastname: Augustine
authors.forename: Praveen
authors.initials: P
authors.lastname: Kumar
authors.forename: Sabu
authors.initials: S
authors.lastname: Thomas
mesh_terms: D000898:Antibiosis; D001407:Bacillus / Q000201:enzymology* / Q000235:genetics; D018441:Biofilms / Q000187:drug effects / Q000254:growth & development*; D002265:Carboxylic Ester Hydrolases / Q000494:pharmacology*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D014734:Vibrio cholerae / Q000187:drug effects* / Q000254:growth & development
chemical_list: D002265:Carboxylic Ester Hydrolases; C432003:N-acyl homoserine lactonase