title: Neonatal colonization of mice with Lactobacillus plantarum producing the aeroallergen Bet v 1 biases towards Th1 and T-regulatory responses upon systemic sensitization.
abstract: BACKGROUND
  The use of recombinant lactic acid bacteria (LAB) as vehicles for mucosal delivery of recombinant allergens is an attractive concept for antigen-defined allergy prevention/treatment. Interventions with LAB are of increasing interest early in life when immune programming is initiated. Here, we investigated the effect of neonatal colonization with a recombinant LAB producing the major birch pollen allergen Bet v 1 in a murine model of type I allergy.
  
  
  METHODS
  We constructed a recombinant Lactobacillus (L.) plantarum NCIMB8826 strain constitutively producing Bet v 1 to be used for natural mother-to-offspring mono-colonization of germ-free BALB/c mice. Allergen-specific immunomodulatory effects of the colonization on humoral and cellular immune responses were investigated prior and after sensitization to Bet v 1.
  
  
  RESULTS
  Mono-colonization with the Bet v 1 producing L. plantarum induced a Th1-biased immune response at the cellular level, evident in IFN-γ production of splenocytes upon stimulation with Bet v 1. After sensitization with Bet v 1 these mice displayed suppressed IL-4 and IL-5 production in spleen and mesenteric lymph node cell cultures as well as decreased allergen-specific antibody responses (IgG1, IgG2a, and IgE) in sera. This suppression was associated with a significant up-regulation of the regulatory marker Foxp3 at the mRNA level in the spleen cells.
  
  
  CONCLUSIONS
  Intervention at birth with a live recombinant L. plantarum producing a clinically relevant allergen reduces experimental allergy and might therefore become an effective strategy for early intervention against the onset of allergic diseases.
authors.affiliation: Department of Immunology and Gnotobiology, Institute of Microbiology of the Academy of Sciences of the Czech Republic, v. v. i., Novy Hradek, Czech Republic.
authors.forename: M
authors.initials: M
authors.lastname: Schwarzer
authors.forename: A
authors.initials: A
authors.lastname: Repa
authors.forename: C
authors.initials: C
authors.lastname: Daniel
authors.forename: I
authors.initials: I
authors.lastname: Schabussova
authors.forename: T
authors.initials: T
authors.lastname: Hrncir
authors.forename: B
authors.initials: B
authors.lastname: Pot
authors.forename: R
authors.initials: R
authors.lastname: Stepankova
authors.forename: T
authors.initials: T
authors.lastname: Hudcovic
authors.forename: A
authors.initials: A
authors.lastname: Pollak
authors.forename: H
authors.initials: H
authors.lastname: Tlaskalova-Hogenova
authors.forename: U
authors.initials: U
authors.lastname: Wiedermann
authors.forename: H
authors.initials: H
authors.lastname: Kozakova
mesh_terms: D000485:Allergens / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D000818:Animals; D000831:Animals, Newborn; D052179:Antigens, Plant / Q000235:genetics / Q000276:immunology*; D029662:Betula / Q000235:genetics / Q000276:immunology; D016207:Cytokines / Q000096:biosynthesis / Q000276:immunology; D051858:Forkhead Transcription Factors / Q000235:genetics / Q000378:metabolism; D006969:Hypersensitivity, Immediate; D007114:Immunization; D007132:Immunoglobulin Isotypes / Q000097:blood / Q000276:immunology; D048191:Lactiplantibacillus plantarum / Q000235:genetics* / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011058:Pollen / Q000235:genetics / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D018417:Th1 Cells / Q000276:immunology*; D018418:Th2 Cells / Q000276:immunology
chemical_list: D000485:Allergens; D052179:Antigens, Plant; D016207:Cytokines; D051858:Forkhead Transcription Factors; C498833:Foxp3 protein, mouse; D007132:Immunoglobulin Isotypes