Expression of the C-terminal ORF2 protein of duck astrovirus for application in a serological test.
Duck astrovirus (DAstV) is an important pathogen causing duck viral hepatitis (DVH), a highly contagious and fatal disease in young ducklings. To provide an antigen for a diagnostic serum test, the C-terminus of DAstV ORF2 protein was expressed in Escherichia coli. Four positive and 30 negative sera were used to validate the purified ORF2 protein by developing an indirect enzyme-linked immunosorbent assay (ELISA). No cross-reactions were found against other duck pathogens, including duck hepatitis A virus, duck plague herpesvirus, duck reovirus, Newcastle disease virus, and Riemerella anatipestifer 12/19 (63.2%) and 26/51 (51%) sera samples from two flocks of ducks that survived DAstV infections in commercial duck farms were positive for DAstV by this method, respectively. Interestingly, DAstV-specific antibodies were also detected in 12 (28.6%) of 42 sera samples from a different flock without DVH, indicating a wide distribution of subclinical infections caused by DAstV.
Wang
Xiaoyan
X
Key Laboratory of Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China.
Wang
Yuzhou
Y
Xie
Xiaoyu
X
Zhang
Bing
B
Zhang
Dabing
D
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral / Q000235:genetics; D019200:Astroviridae / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D019350:Astroviridae Infections / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D004372:Ducks; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D004926:Escherichia coli / Q000235:genetics; D011201:Poultry Diseases / Q000175:diagnosis* / Q000821:virology*; D011994:Recombinant Proteins / Q000235:genetics; D012680:Sensitivity and Specificity; D014773:Virology / Q000379:methods*
D000914:Antibodies, Viral; D000956:Antigens, Viral; D011994:Recombinant Proteins