Stereoselective determination of drugs and metabolites in body fluids, tissues and microsomal preparations by capillary electrophoresis (2000-2010).
During the past two decades, chiral capillary electrophoresis (CE) emerged as a promising, effective and economic approach for the enantioselective determination of drugs and their metabolites in body fluids, tissues and in vitro preparations. This review discusses the principles and important aspects of CE-based chiral bioassays, provides a survey of the assays developed during the past 10 years and presents an overview of the key achievements encountered in that time period. Applications discussed encompass the pharmacokinetics of drug enantiomers in vivo and in vitro, the elucidation of the stereoselectivity of drug metabolism in vivo and in vitro, and bioanalysis of drug enantiomers of toxicological, forensic and doping interest. Chiral CE was extensively employed for research purposes to investigate the stereoselectivity associated with hydroxylation, dealkylation, carboxylation, sulfoxidation, N-oxidation and ketoreduction of drugs and metabolites. Enantioselective CE played a pivotal role in many biomedical studies, thereby providing new insights into the stereoselective metabolism of drugs in different species which might eventually lead to new strategies for optimization of pharmacotherapy in clinical practice.
Caslavska
Jitka
J
Department of Clinical Pharmacology and Visceral Research, University of Bern, Murtenstrasse 35, CH-3010 Bern, Switzerland.
Thormann
Wolfgang
W
D001826:Body Fluids / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D013287:Illicit Drugs / Q000032:analysis / Q000493:pharmacokinetics; D007649:Ketamine; D004364:Pharmaceutical Preparations / Q000032:analysis*; D010599:Pharmacokinetics; D013237:Stereoisomerism
D013287:Illicit Drugs; D004364:Pharmaceutical Preparations; D007649:Ketamine