title: Successful transplant of mesenchymal stem cells in induced osteonecrosis of the ovine femoral head: preliminary results.
abstract: OBJECTIVE
  Evaluate the bone tissue recovery following transplantation of ovine mesenchymal stem cells (MSC) from bone marrow and human immature dental-pulp stem cells (hIDPSC) in ovine model of induced osteonecrosis of femoral head (ONFH).
  
  
  METHODS
  Eight sheep were divided in three experimental groups. First group was composed by four animals with ONFH induced by ethanol through central decompression (CD), for control group without any treatment. The second and third group were compose by two animals, six weeks after ONFH induction received transplantation of heterologous ovine MSC (CD + oMSC), and hIDPSC (CD + hIDPSC), respectively. In both experiments the cells were transplanted without application of any type of immunosupression protocol.
  
  
  RESULTS
  Our data indicate that both cell types used in experiments were able to proliferate within injured site providing bone tissue recovery. The histological results obtained from CD+hIDPSC suggested that the bone regeneration in such animals was better than that observed in CD animals.
  
  
  CONCLUSIONS
  Mesenchymal stem cell transplant in induced ovine osteonecrosis of femoral head by central decompression technique is safe, and apparently favors bone regeneration of damaged tissues.
authors.affiliation: Department of Surgery, Faculty of Veterinary Medicine and Animal Science, USP, Sao Paulo, Brazil. mtajra@gmail.com
authors.forename: Matheus Levi Tajra
authors.initials: ML
authors.lastname: Feitosa
authors.forename: Leandro
authors.initials: L
authors.lastname: Fadel
authors.forename: Patrícia Cristina Baleeiro
authors.initials: PC
authors.lastname: Beltrão-Braga
authors.forename: Cristiane Valverde
authors.initials: CV
authors.lastname: Wenceslau
authors.forename: Irina
authors.initials: I
authors.lastname: Kerkis
authors.forename: Alexandre
authors.initials: A
authors.lastname: Kerkis
authors.forename: Eduardo Harry
authors.initials: EH
authors.lastname: Birgel Júnior
authors.forename: João Flávio Panattoni
authors.initials: JF
authors.lastname: Martins
authors.forename: Daniele dos Santos
authors.initials: Ddos S
authors.lastname: Martins
authors.forename: Maria Angélica
authors.initials: MA
authors.lastname: Miglino
authors.forename: Carlos Eduardo
authors.initials: CE
authors.lastname: Ambrósio
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000009:adverse effects; D003782:Dental Pulp / Q000637:transplantation*; D004195:Disease Models, Animal; D005271:Femur Head Necrosis / Q000601:surgery*; D006801:Humans; D045164:Mesenchymal Stem Cell Transplantation / Q000009:adverse effects; D011897:Random Allocation; D012756:Sheep; D014183:Transplantation, Heterologous
chemical_list: D001672:Biocompatible Materials