Collagenase-3 expression by tumor cells and gelatinase B expression by stromal fibroblast-like cells are associated with biochemical recurrence after radical prostatectomy in patients with prostate cancer.
OBJECTIVE
To investigate the possible clinical value of the expression of MMPs and their tissue inhibitors (TIMPs) by the different cellular types of the tumor scenario to predict biochemical recurrence in patients undergoing radical prostatectomy due clinically localized prostate cancer.


METHODS
An immunohistochemical study was performed using tissue arrays and specific antibodies against MMPs-1, 2, 7, 9, 11, 13 and 14 and TIMPs-1, 2 and 3 on cancer specimens from 133 patients with clinical localized prostate carcinoma.


RESULTS
Immunostaining for all the proteins studied was localized predominantly in tumor cells, but also in stromal cells in a significant percentage of prostate carcinomas, ranged from 20 to 50% for several proteins in fibroblast-like cells and in mononuclear inflammatory cells. Multivariate analysis according to a Cox model demonstrated that tumor stage (P < 0.0001) and Gleason grading (grades 7-10: 2.08 (1.1-3.9); P < 0.05) were significantly and independently associated with biochemical recurrence. Additionally, the expression of MMP-9 by fibroblast-like cells (P < 0.01) and MMP-13 by tumor cells (P < 0.05) were also variables significantly and independently associated with biochemical recurrence.


CONCLUSIONS
MMP-13 expression by tumor cells and MMP-9 by stromal fibroblast-like cells were independent factors of biochemical recurrence in prostate cancer.
Escaff
Safwan
S
Servicio Urología, Hospital de Jove, Gijón, Spain.
Fernández
Jesús M
JM
González
Luis O
LO
Suárez
Aurelio
A
González-Reyes
Salomé
S
González
José M
JM
Vizoso
Francisco J
FJ
D000368:Aged; D006801:Humans; D008297:Male; D053509:Matrix Metalloproteinase 13 / Q000096:biosynthesis*; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis*; D020782:Matrix Metalloproteinases / Q000096:biosynthesis; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local / Q000378:metabolism*; D017430:Prostate-Specific Antigen / Q000097:blood; D011468:Prostatectomy; D011471:Prostatic Neoplasms / Q000097:blood / Q000378:metabolism* / Q000601:surgery*; D017154:Stromal Cells / Q000378:metabolism*; D019714:Tissue Inhibitor of Metalloproteinases / Q000096:biosynthesis
D019714:Tissue Inhibitor of Metalloproteinases; D017430:Prostate-Specific Antigen; D053509:Matrix Metalloproteinase 13; D020782:Matrix Metalloproteinases; D020780:Matrix Metalloproteinase 9