[Localization of enamelin in developing rat incisor by immunofluorescence].
OBJECTIVE
To investigate the expression of enamelin in developing rat incisor.


METHODS
Immunofluorescence staining for the specimens of developing rat incisor was used to localize the expression of enamelin.


RESULTS
Enamelin was not detected at the proliferation phase, and early differentiation phase in preameloblasts. At the advanced differentiation phase, enamelin expressed weakly in ameloblasts and odotoblasts. At the early maturation phase enamelin was detected in ameloblasts and odotoblasts. Enamelin expressed strongly in ameloblasts and enamel matrix but strongly in odotoblasts at the intermediate maturation phase and weakly at the advanced maturation phase. Enamelin was not found in the other stages and position.


CONCLUSIONS
Enamelin,involved in enamel and dentin matrix formation, may play an important role in signal transduction of matrix formation. Supported by Project of the "Tenth-Five Year" Science Support Plans and Project "211" of Tongji University (Grant No.1504102011).
Liu
Tian-lin
TL
Department of Stomatology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200120, Hospital of Stomatology, Tongji University, Shanghai 200072, China. tanlionone@yahoo.com.cn
Wang
Qiang
Q
D000565:Ameloblasts; D000818:Animals; D002454:Cell Differentiation; D003746:Dental Enamel Proteins; D005455:Fluorescent Antibody Technique; D007180:Incisor; D051381:Rats
D003746:Dental Enamel Proteins; C038456:tuftelin