Aldolase A-HBsAg interaction and its effect on ultraviolet radiation induced apoptosis in 293FT cells.
OBJECTIVE
Hepatitis B virus (HBV) infection poses great challenges to humans, claiming one million lives annually worldwide. Solid data have related HBV to hepatocellular carcinoma.


METHODS
In the present research, we verified the interaction between surface protein (HBs) encoded by HBV and aldolase A (ALDA) using yeast two-hybrid, mammalian two-hybrid, co-immunoprecipitation, GST pull-down and laser scanning confocal.


RESULTS
Anti-ALDA antibody precipitated Gal4-HBs fusion protein in the presence of HBs. Anti-HBs antibody precipitated p65ΔN-ALDA only in the presence of ALDA. Small HBs could be pulled down by GST-ALDA. Cells transfected with pCMV-AD-ALDA showed a protection from ultraviolet radiation-induced apoptosis (21.3% ± 1.3% for ALDA, 35.4% ± 2.1% for control, P < 0.05).


CONCLUSIONS
An interaction does exist between ALDA and HBs. The S region within HBs is sufficient for binding ALDA. In addition, ALDA conferred protection to ultraviolet radiation-induced apoptosis, and this effect was enhanced by the interaction between HBs and ALDA.
Pan
Jin-Shui
JS
Division of Gastroenterology, Zhongshan Hospital Xiamen University, and Gastroenterology Institute of Xiamen University, and Gastroenterology Center of Xiamen, Xiamen, Fujian Province, China.
Zhou
Fei
F
Xie
Chen-Xi
CX
Cai
Jia-Yan
JY
Chen
Jian-Min
JM
Zhang
Zhi-Ping
ZP
Dong
Jing
J
Xu
Hong-Zhi
HZ
Shi
Hua-Xiu
HX
Ren
Jian-Lin
JL
D000818:Animals; D017209:Apoptosis / Q000276:immunology / Q000528:radiation effects*; D015153:Blotting, Western; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D002460:Cell Line; D006224:Cricetinae; D004247:DNA / Q000032:analysis; D004273:DNA, Neoplasm / Q000235:genetics; D005434:Flow Cytometry; D005634:Fructose-Bisphosphate Aldolase / Q000276:immunology*; D006514:Hepatitis B Surface Antigens / Q000276:immunology*; D019694:Hepatitis B, Chronic / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D018613:Microscopy, Confocal; D016133:Polymerase Chain Reaction; D014466:Ultraviolet Rays
D004273:DNA, Neoplasm; D006514:Hepatitis B Surface Antigens; D004247:DNA; D005634:Fructose-Bisphosphate Aldolase