title: Quantification of drugs of abuse in municipal wastewater via SPE and direct injection liquid chromatography mass spectrometry.
abstract: We present an isotopic-dilution direct injection reversed-phase liquid chromatography-tandem mass spectrometry method for the simultaneous determination of 23 drugs of abuse, drug metabolites, and human-use markers in municipal wastewater. The method places particular emphasis on cocaine; it includes 11 of its metabolites to facilitate assessment of routes of administration and to enhance the accuracy of estimates of cocaine consumption. Four opioids (6-acetylmorphine, morphine, hydrocodone, and oxycodone) are also included, along with five phenylamine drugs (amphetamine, methamphetamine, 3,4-methylenedioxy-methamphetamine, methylbenzodioxolyl-butanamine, and 3,4-methylenedioxy-N-ethylamphetamine) and two human-use markers (cotinine and creatinine). The method is sufficiently sensitive to directly quantify (without preconcentration) 18 analytes in wastewater at concentrations less than 50 ng/L. We also present a modified version of this method that incorporates solid-phase extraction to further enhance sensitivity. The method includes a confirmatory LC separation (selected by evaluating 13 unique chromatographic phases) that has been evaluated using National Institute of Standards and Technology Standard Reference Material 1511 Multi-Drugs of Abuse in Freeze-Dried Urine. Seven analytes (ecgonine methyl ester, ecgonine ethyl ester, anhydroecgonine methyl ester, m-hydroxybenzoylecgonine, p-hydroxybenzoyl-ecgonine, ecgonine, and anhydroecgonine) were detected for the first time in a wastewater sample.
authors.affiliation: Department of Geography and Environmental Engineering, Johns Hopkins University, 3400 North Charles Street, Baltimore, MD 21218, USA.
authors.forename: Kevin J
authors.initials: KJ
authors.lastname: Bisceglia
authors.forename: A Lynn
authors.initials: AL
authors.lastname: Roberts
authors.forename: Michele M
authors.initials: MM
authors.lastname: Schantz
authors.forename: Katrice A
authors.initials: KA
authors.lastname: Lippa
mesh_terms: D056148:Chromatography, Reverse-Phase / Q000295:instrumentation / Q000379:methods; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D013287:Illicit Drugs / Q000032:analysis*; D012722:Sewage / Q000032:analysis*; D052616:Solid Phase Extraction; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation / Q000379:methods; D019966:Substance-Related Disorders / Q000453:epidemiology; D053719:Tandem Mass Spectrometry / Q000295:instrumentation / Q000379:methods; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D013287:Illicit Drugs; D012722:Sewage; D014874:Water Pollutants, Chemical