Microfluidic droplets: new integrated workflows for biological experiments.
Miniaturization of the classical test tube to picoliter dimensions is possible in monodisperse water-in-oil droplets that are generated in microfluidic devices. The establishment of standard unit operations for droplet handling and the ability to carry out experiments with DNA, proteins, cells and organisms provides the basis for the design of more complex workflows to address biological challenges. The emerging experimental format makes possible a quantitative readout for large numbers of experiments with a precision comparable to the macroscopic scale. Directed evolution, diagnostics and compound screening are areas in which the first steps are being taken toward the long-term goal of transforming the way we design and carry out experiments.
Kintses
Balint
B
Department of Biochemistry, University of Cambridge, United Kingdom.
van Vliet
Liisa D
LD
Devenish
Sean R A
SR
Hollfelder
Florian
F
D000818:Animals; D001695:Biology / Q000295:instrumentation*; D003933:Diagnosis; D019020:Directed Molecular Evolution; D004353:Drug Evaluation, Preclinical; D057166:High-Throughput Screening Assays; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000379:methods*