title: Effect of simvastatin treatment on rat livers subjected to ischemia/reperfusion.
abstract: We evaluated the effect of simvastatin (SV) on the oxido-redox state in rat livers submitted to ischemia-reperfusion (I/R). Rats received SV (groups: S, S-IR) or saline solution (groups: C, C-IR) intragastrically (25 mg/kg) for 21 days. Before homogenization, rat livers (C-IR, S-IR) underwent ischemia (40 min) and reperfusion (60 min). Activities of such antioxidative enzymes as superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) as well as lipid peroxides (LPO) level as indicator of oxidative stress were then estimated in the homogenates. All these parameters were measured spectrophotometrically. Additionally, alanine and asparagine aminotransferase (ALT, AST) levels were estimated in the blood before and after I/R. In groups C and S all examined parameters were similar regardless of SV-treatment. I/R produced significant increases in GPx and CAT activities only in the C-IR group. Conversely, GPx activity was significantly decreased and ALT and AST increased significantly in the S-IR group. SV did not evoke any noticeable protective changes in rat livers after 3 weeks of treatment. After I/R, some of the observed properties could suggest that SV may have even made liver function and the oxidative state worse.
authors.affiliation: Department of Pharmacology, Wroclaw Medical University, Mikulicza Radeckiego 2, PL 50-345 Wrocław, Poland. malgorzata.trocha@gmail.com
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Trocha
authors.forename: Anna
authors.initials: A
authors.lastname: Merwid-Ląd
authors.forename: Ewa
authors.initials: E
authors.lastname: Chlebda
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Pieśniewska
authors.forename: Tomasz
authors.initials: T
authors.lastname: Sozański
authors.forename: Adam
authors.initials: A
authors.lastname: Szeląg
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001219:Aspartate Aminotransferases / Q000097:blood; D002374:Catalase / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors / Q000494:pharmacology* / Q000633:toxicity; D008099:Liver / Q000187:drug effects* / Q000473:pathology; D008111:Liver Function Tests; D008297:Male; D010084:Oxidation-Reduction / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000150:complications*; D019821:Simvastatin / Q000494:pharmacology* / Q000633:toxicity
chemical_list: D000975:Antioxidants; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D019821:Simvastatin; D002374:Catalase; D005979:Glutathione Peroxidase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase