In vitro modulation of caprine monocyte immune functions by ω-3 polyunsaturated fatty acids.
The in vitro effects of the ω-3 polyunsaturated fatty acids (PUFAs) eicosapentanoic acid (EPA) and docosahexanoic acid (DHA) on phagocytosis and the extracellular respiratory burst in caprine monocytes were assessed. Blood monocytes incubated with increasing concentrations of EPA or DHA (25-200 μM) demonstrated increased phagocytosis compared to unexposed monocytes. Generation of reactive oxygen species (ROS) was not markedly affected in the presence of EPA and DHA, except at 200 μM, at which concentrations monocyte viability was also reduced.
Lecchi
Cristina
C
Dipartimento di Patologia Animale, Igiene e Sanità Pubblica Veterinaria, Università di Milano, via Celoria 10, 20133 Milano, Italy.
Invernizzi
Guido
G
Agazzi
Alessandro
A
Ferroni
Mariella
M
Pisani
Laura Francesca
LF
Savoini
Giovanni
G
Ceciliani
Fabrizio
F
D000818:Animals; D004281:Docosahexaenoic Acids / Q000494:pharmacology*; D015118:Eicosapentaenoic Acid / Q000494:pharmacology*; D006041:Goats / Q000276:immunology*; D009000:Monocytes / Q000276:immunology* / Q000378:metabolism; D010587:Phagocytosis; D017382:Reactive Oxygen Species / Q000276:immunology* / Q000378:metabolism; D016897:Respiratory Burst
D017382:Reactive Oxygen Species; D004281:Docosahexaenoic Acids; D015118:Eicosapentaenoic Acid