Sequencing of multiple clostridial genomes related to biomass conversion and biofuel production.
Modern methods to develop microbe-based biomass conversion processes require a system-level understanding of the microbes involved. Clostridium species have long been recognized as ideal candidates for processes involving biomass conversion and production of various biofuels and other industrial products. To expand the knowledge base for clostridial species relevant to current biofuel production efforts, we have sequenced the genomes of 20 species spanning multiple genera. The majority of species sequenced fall within the class III cellulosome-encoding Clostridium and the class V saccharolytic Thermoanaerobacteraceae. Species were chosen based on representation in the experimental literature as model organisms, ability to degrade cellulosic biomass either by free enzymes or by cellulosomes, ability to rapidly ferment hexose and pentose sugars to ethanol, and ability to ferment synthesis gas to ethanol. The sequenced strains significantly increase the number of noncommensal/nonpathogenic clostridial species and provide a key foundation for future studies of biomass conversion, cellulosome composition, and clostridial systems biology.
Hemme
Christopher L
CL
Department of Botany and Microbiology, University of Oklahoma, Norman, Oklahoma 73019, USA.
Mouttaki
Housna
H
Lee
Yong-Jin
YJ
Zhang
Gengxin
G
Goodwin
Lynne
L
Lucas
Susan
S
Copeland
Alex
A
Lapidus
Alla
A
Glavina del Rio
Tijana
T
Tice
Hope
H
Saunders
Elizabeth
E
Brettin
Thomas
T
Detter
John C
JC
Han
Cliff S
CS
Pitluck
Sam
S
Land
Miriam L
ML
Hauser
Loren J
LJ
Kyrpides
Nikos
N
Mikhailova
Natalia
N
He
Zhili
Z
Wu
Liyou
L
Van Nostrand
Joy D
JD
Henrissat
Bernard
B
He
Qiang
Q
Lawson
Paul A
PA
Tanner
Ralph S
RS
Lynd
Lee R
LR
Wiegel
Juergen
J
Fields
Matthew W
MW
Arkin
Adam P
AP
Schadt
Christopher W
CW
Stevenson
Bradley S
BS
McInerney
Michael J
MJ
Yang
Yunfeng
Y
Dong
Hailiang
H
Xing
Defeng
D
Ren
Nanqi
N
Wang
Aijie
A
Huhnke
Raymond L
RL
Mielenz
Jonathan R
JR
Ding
Shi-You
SY
Himmel
Michael E
ME
Taghavi
Safiyh
S
van der Lelie
Daniël
D
Rubin
Edward M
EM
Zhou
Jizhong
J
D056804:Biofuels; D018533:Biomass; D003013:Clostridium / Q000235:genetics* / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D016680:Genome, Bacterial; D008969:Molecular Sequence Data; D046968:Thermoanaerobacter / Q000235:genetics*
D056804:Biofuels