Calcium flux between the endoplasmic reticulum and mitochondrion contributes to poliovirus-induced apoptosis.
We show that poliovirus (PV) infection induces an increase in cytosolic calcium (Ca(2+)) concentration in neuroblastoma IMR5 cells, at least partly through Ca(2+) release from the endoplasmic reticulum lumen via the inositol 1,4,5-triphosphate receptor (IP(3)R) and ryanodine receptor (RyR) channels. This leads to Ca(2+) accumulation in mitochondria through the mitochondrial Ca(2+) uniporter and the voltage-dependent anion channel (VDAC). This increase in mitochondrial Ca(2+) concentration in PV-infected cells leads to mitochondrial dysfunction and apoptosis.
Brisac
Cynthia
C
Institut Pasteur, Unité de Biologie des Virus Entériques, 25 rue du Dr Roux, Paris, France.
Téoulé
François
F
Autret
Arnaud
A
Pelletier
Isabelle
I
Colbère-Garapin
Florence
F
Brenner
Catherine
C
Lemaire
Christophe
C
Blondel
Bruno
B
D017209:Apoptosis / Q000502:physiology*; D015153:Blotting, Western; D002118:Calcium / Q000378:metabolism*; D002458:Cell Fractionation; D045744:Cell Line, Tumor; D003600:Cytosol / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D005434:Flow Cytometry; D006801:Humans; D053496:Inositol 1,4,5-Trisphosphate Receptors / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism*; D028361:Mitochondrial Diseases / Q000209:etiology*; D011051:Poliomyelitis / Q000150:complications* / Q000378:metabolism; D017955:Poliovirus; D019837:Ryanodine Receptor Calcium Release Channel / Q000378:metabolism
D053496:Inositol 1,4,5-Trisphosphate Receptors; D019837:Ryanodine Receptor Calcium Release Channel; D002118:Calcium