title: The intrinsically disordered late embryogenesis abundant protein LEA18 from Arabidopsis thaliana modulates membrane stability through binding and folding.
abstract: Intrinsically disordered proteins (IDPs) constitute a substantial part of cellular proteomes. Late embryogenesis abundant (LEA) proteins are mostly predicted to be IDPs associated with dehydration tolerance in many plant, animal and bacterial species. Their functions, however, are largely unexplored and also their structure and interactions with potential target molecules have only recently been experimentally investigated in a small number of proteins. Here, we report on the structure and interactions with membranes of the Pfam LEA_1 protein LEA18 from the higher plant Arabidopsis thaliana. This functionally uncharacterized positively charged protein specifically aggregated and destabilized negatively charged liposomes. Isothermal titration calorimetry showed binding of the protein to both charged and uncharged membranes. LEA18 alone was largely unstructured in solution. While uncharged membranes had no influence on the secondary structure of LEA18, the protein partially folded into β-sheet structure in the presence of negatively charged liposomes. These data suggest that LEA18 does not function as a membrane stabilizing protein, as suggested for other LEA proteins. Instead, a possible function of LEA18 could be the composition-dependent modulation of membrane stability, e.g., during signaling or vesicle-mediated transport.
authors.affiliation: Max-Planck-Institut für Molekulare Pflanzenphysiologie, Am Mühlenberg 1, D-14476 Potsdam, Germany.
authors.forename: Michaela
authors.initials: M
authors.lastname: Hundertmark
authors.forename: Rumiana
authors.initials: R
authors.lastname: Dimova
authors.forename: Jan
authors.initials: J
authors.lastname: Lengefeld
authors.forename: Robert
authors.initials: R
authors.lastname: Seckler
authors.forename: Dirk K
authors.initials: DK
authors.lastname: Hincha
mesh_terms: D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D001703:Biophysics / Q000379:methods; D002151:Calorimetry / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D008081:Liposomes / Q000737:chemistry; D010316:Particle Size; D018521:Plant Physiological Phenomena; D011485:Protein Binding; D011487:Protein Conformation; D017510:Protein Folding; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000737:chemistry; D013997:Time Factors
chemical_list: D029681:Arabidopsis Proteins; C556259:LEA18 protein, Arabidopsis; D008081:Liposomes; D011994:Recombinant Proteins