title: Development of an in vitro model to study the impact of BMP-2 on metastasis to bone.
abstract: Prostate cancer cases and deaths have increased for years, yet the mechanisms involved in prostate cancer metastasis to bone remain poorly understood. To address this need, an effective and relevant in vitro model for the study of prostate cancer bone metastases would be useful. Therefore, a 3D in vitro tissue system was established using prostate cancer cells (PC3), suitable culture conditions and a 3D silk scaffold biomaterial to provide mechanically robust and slow degrading matrices to support the tissues for extended time frames. The role of BMP-2 on the progression of prostate cancer was investigated using this 3D tissue system. The results suggest that BMP-2 stimulates the migration of PC3 cells, suggesting insight into mechanisms involved in this critical step in the disease. The data support the conclusion that this in vitro system mimics aspects of prostate cancer metastasis in the presence of BMP-2, thus the system can be utilized as a starting point as an in vitro model for further studies of prostate cancer development and metastasis, as well as in the screening of new therapeutic treatments.
authors.affiliation: Department of Biomedical Engineering, Tufts University, Medford, MA 02155, USA.
authors.forename: Heenam
authors.initials: H
authors.lastname: Kwon
authors.forename: Hyeon Joo
authors.initials: HJ
authors.lastname: Kim
authors.forename: William L
authors.initials: WL
authors.lastname: Rice
authors.forename: Balajikarthick
authors.initials: B
authors.lastname: Subramanian
authors.forename: Sang-Hyug
authors.initials: SH
authors.lastname: Park
authors.forename: Irene
authors.initials: I
authors.lastname: Georgakoudi
authors.forename: David L
authors.initials: DL
authors.lastname: Kaplan
mesh_terms: D055396:Bone Morphogenetic Protein 2 / Q000378:metabolism*; D001859:Bone Neoplasms / Q000378:metabolism* / Q000473:pathology / Q000556:secondary*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D004797:Enzyme-Linked Immunosorbent Assay; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D008297:Male; D018613:Microscopy, Confocal; D008954:Models, Biological; D047011:Silk / Q000494:pharmacology; D054457:Tissue Scaffolds
chemical_list: D055396:Bone Morphogenetic Protein 2; D047011:Silk