title: On-line concentration and determination of tobacco-specific N-nitrosamines by cation-selective exhaustive injection-sweeping-micellar electrokinetic chromatography.
abstract: In this paper, a micellar electrokinetic chromatography (MEKC) method combined with cation-selective exhaustive injection (CSEI) and sweeping was developed to separate and concentrate four tobacco-specific N-nitrosamines (TSNAs) including N'-nitrosoanabasine (NAB), 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK), 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL) and 4-(methylnitrosamino)-4-(3-pyridyl)-1-butanol (iso-NNAL). Experimental parameters affecting separation efficiency and enhancement factors were investigated in detail. Under the optimum MEKC condition, NAB, NNK, NNAL and iso-NNAL were baseline separated with high separation efficiencies and good peak shapes. Furthermore, with the preconcentration by CSEI-sweeping-MEKC, the sensitivity enhancement factors for NAB, NNK, NNAL and iso-NNAL in terms of peak areas ranged from 6.0×10(3) to 1.5×10(4), and the detection limits (LOD, S/N=3) of four TSNAs were in the range of 0.004-0.016μg/mL. In addition, this method had fairly good repeatability, and the RSDs of retention time and peak area were less than 1% and 5%, respectively. Finally, this method showed promising capabilities in the application of detecting and analyzing TSNAs in human urine samples.
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Institute of Analytical Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.
authors.forename: Youyou
authors.initials: Y
authors.lastname: Yang
authors.forename: Honggang
authors.initials: H
authors.lastname: Nie
authors.forename: Chenchen
authors.initials: C
authors.lastname: Li
authors.forename: Yu
authors.initials: Y
authors.lastname: Bai
authors.forename: Ning
authors.initials: N
authors.lastname: Li
authors.forename: Jie
authors.initials: J
authors.lastname: Liao
authors.forename: Huwei
authors.initials: H
authors.lastname: Liu
mesh_terms: D002273:Carcinogens / Q000032:analysis* / Q000737:chemistry; D002412:Cations / Q000737:chemistry; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D066298:In Vitro Techniques; D015394:Molecular Structure; D009602:Nitrosamines / Q000032:analysis* / Q000737:chemistry / Q000652:urine; D012680:Sensitivity and Specificity; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D013997:Time Factors; D014026:Nicotiana / Q000737:chemistry*
chemical_list: D002273:Carcinogens; D002412:Cations; D009602:Nitrosamines; D012967:Sodium Dodecyl Sulfate