title: Mitigation of arsenic-mediated renal oxidative stress in rat by Pleurotus florida lectin.
abstract: Oyster mushroom, Pleurotus florida is regarded as one of the popular food with biopharmaceutical properties. Here, the study aimed to investigate the antioxidative effects of mushroom (Pleurotus florida) lectin against arsenic-induced nephrotoxicity in rats. Animals were divided into four groups; Group 1 was control. Groups 2, 3 and 4 were exposed to arsenic (20 parts per million [ppm] in drinking water), arsenic plus oral supplementation of ascorbic acid (25 mg/kg body weight) and arsenic plus oral supplementation of mushroom lectin (150 mg/kg body weight) respectively. Both ascorbic acid and mushroom lectin prevented the arsenic-mediated growth retardation and normalized the elevated kidney weight. Disrupted activities of superoxide dismutase (SOD) and catalase (CAT) and enhanced lipid peroxidation (LPO), protein carbonyl (PC) and nitric oxides (NO) production in kidney caused by arsenic could also be maintained towards normalcy by supplementation of mushroom lectin and ascorbic acid. These antioxidative effects were exhibited in a time-dependant manner. Further, arsenic-mediated down-regulation of messenger RNA (mRNA) expression of superoxide dismutase 2 (SOD(2)) gene was obstructed by these agents. Thus it was found that mushroom lectin reversed the effect of arsenic-mediated oxidative stress in a time-dependent manner.
authors.affiliation: Indian Veterinary Research Institute, Eastern Regional Station, Kolkata, India. asitmed2000@yahoo.com
authors.forename: Asit Kumar
authors.initials: AK
authors.lastname: Bera
authors.forename: Tanmoy
authors.initials: T
authors.lastname: Rana
authors.forename: Subhashree
authors.initials: S
authors.lastname: Das
authors.forename: Debasis
authors.initials: D
authors.lastname: Bhattacharya
authors.forename: Diganta
authors.initials: D
authors.lastname: Pan
authors.forename: Subhasish
authors.initials: S
authors.lastname: Bandyopadhyay
authors.forename: Subrata Kumar
authors.initials: SK
authors.lastname: Das
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000302:isolation & purification / Q000627:therapeutic use*; D020261:Arsenic Poisoning / Q000188:drug therapy* / Q000201:enzymology / Q000378:metabolism / Q000473:pathology; D001835:Body Weight / Q000187:drug effects; D002374:Catalase / Q000378:metabolism; D015536:Down-Regulation; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D007668:Kidney / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism / Q000473:pathology; D037102:Lectins / Q000302:isolation & purification / Q000627:therapeutic use*; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism; D009929:Organ Size / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D020076:Pleurotus / Q000737:chemistry*; D050050:Protein Carbonylation; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D013997:Time Factors; D000099156:Superoxide Dismutase 2
chemical_list: D000975:Antioxidants; D002374:Catalase; D037102:Lectins; D009569:Nitric Oxide; D013482:Superoxide Dismutase; D000099156:Superoxide Dismutase 2