Fixation and preparation of developing tissues from Aedes aegypti.
Blood-feeding mosquitoes, including the dengue and yellow fever vector Aedes aegypti, transmit many of the world's deadliest diseases. Such diseases have resurged in developing countries and pose clear threats for epidemic outbreaks in developed countries. Recent mosquito genome projects have stimulated interest in the potential for arthropod-borne disease control by genetic manipulation of vector insects, and genes that regulate development are of particular interest. This protocol describes a method for fixation and dissection of Ae. aegypti embryos, larvae, and pupae. Tissue processed in this manner can be used subsequently for in situ hybridization detection of mRNA or immunohistochemical analysis of protein expression.
Clemons
Anthony
A
Department of Medical and Molecular Genetics, Indiana University School of Medicine, South Bend, Indiana 46617, USA.
Haugen
Morgan
M
Flannery
Ellen
E
Kast
Kristopher
K
Jacowski
Caitlin
C
Severson
David
D
Duman-Scheel
Molly
M
D000330:Aedes / Q000166:cytology* / Q000196:embryology / Q000254:growth & development; D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D007303:Insect Vectors / Q000166:cytology* / Q000196:embryology / Q000254:growth & development; D016707:Tissue Fixation / Q000379:methods*