Recombinant domain V of β2-glycoprotein I inhibits the formation of a 7-ketocholesteryl-9-carboxynonanoate and β2-glycoprotein I complex.
Our prior study has been reported the formation of the oxidized low-density lipoprotein (oxLDL)/β(2)-glycoproteinI (β(2)-GPI)/autoantibody complex facilitated the antiphospholipid syndrome (APS) process. The domain V of β(2)-GPI binds to the negatively charged molecules, e.g. 7-ketochoresteryl-9-caboxynonanoate (oxLig-1) derived from the oxLDL and mediates the interaction between oxLDL and β(2)-GPI. In the present study, the oxLig-1/β(2)-GPI/anti-β(2)-GPI Ab (WB-CAL-1) model was established. The recombinant domain V of β(2)-GPI (rβ(2)-GPI DV) expressed in Escherichia coli competitively inhibits the interaction between β(2)-GPI and oxLig-1 in the enzyme-linked immunoassay. Moreover, the rβ(2)-GPI DV significantly inhibits the formation of the oxLig-1/β(2)-GPI/autoantibody complex in an APS patient. The present work suggests a novel possibility that rβ(2)-GPI DV could be used to inhibit the formation of oxLDL/β(2)-GPI/autoantibody complex, and give us a hint for the development of new therapeutic strategies to prevent the APS process.
Zhang
Yingbiao
Y
College of Bioengineering, Key Laboratory of Bio-organic Chemistry, Dalian University, 10-Xuefu Avenue, Dalian Economical and Technological Development Zone, Liaoning 116622, China.
Li
Wenzhe
W
Chi
Yan
Y
Wang
Renjun
R
Wang
Dan
D
Zhang
Fan
F
Liu
Zhe
Z
Matsuura
Eiji
E
Liu
Qingping
Q
D016736:Antiphospholipid Syndrome / Q000097:blood / Q000276:immunology; D001323:Autoantibodies / Q000737:chemistry; D002308:Cardiolipins / Q000737:chemistry; D002788:Cholesterol Esters / Q000737:chemistry*; D006801:Humans; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry*; D053482:beta 2-Glycoprotein I / Q000737:chemistry* / Q000276:immunology
C510905:7-ketocholesteryl-9-carboxynonanoate; D001323:Autoantibodies; D002308:Cardiolipins; D002788:Cholesterol Esters; D011994:Recombinant Proteins; D053482:beta 2-Glycoprotein I