Identifying respiratory viruses in nasal mucus from children.
Large amounts of respiratory viruses are shed in nasal secretions by children. Nasal mucus was compared with nasopharyngeal swabs as a source for respiratory virus testing. Multiplex reverse transcription-polymerase chain reaction detected virus in nasal mucus specimens in 73% (11/15) of positive cases, demonstrating the potential utility of less invasive specimens when a highly sensitive method is used for respiratory virus detection.
She
Rosemary C
RC
Department of Pathology, University of Utah School of Medicine, Salt Lake City, UT, USA. rosemary.she-bender@hsc.utah.edu
Taggart
Edward W
EW
Ruegner
Roland
R
Hymas
Weston C
WC
Bender
Jeffrey M
JM
Weir
Peter
P
Petti
Cathy A
CA
D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D009093:Mucus / Q000821:virology*; D009297:Nasal Mucosa / Q000821:virology*; D009305:Nasopharynx / Q000821:virology; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D014773:Virology / Q000379:methods*; D017201:Virus Shedding; D014780:Viruses / Q000145:classification* / Q000302:isolation & purification*