Microbial treatment of high-strength perchlorate wastewater.
To treat wastewater containing high concentrations of perchlorate, a perchlorate reducing-bacterial consortium was obtained by enrichment culture grown on high-strength perchlorate (1200 mg L(-1)) feed medium, and was characterized in a sequence batch reactor (SBR) over a long-time operation. The consortium removed perchlorate in the SBR with high reduction rates (35-90 mg L(-1)h(-1)) and stable removal efficiency over 200-day operations. The maximum specific perchlorate reduction rate (qmax), half saturation constant (Ks), and optimal pH range were 0.67 mg-perchlorate mg-dry cell weight(-1) h(-1), 193.8 mg-perchlorate L(-1), and pH 7-9, respectively. The perchlorate reduction yield was 0.48 mol-perchloratemol-acetate(-1). A clone library prepared using the amplicons of cld gene encoding chlorate dismutase showed that the dominant (per)chlorate reducing bacteria in the consortium were Dechlorosoma sp. (53%), Ideonella sp. (28%), and Dechloromonas sp. (19%).
Nor
Seong Jin
SJ
Department of Chemical Engineering, Soongsil University, Seoul 156-743, South Korea.
Lee
Sang Hyon
SH
Cho
Kyung-Suk
KS
Cha
Daniel K
DK
Lee
Kang In
KI
Ryu
Hee Wook
HW
D000085:Acetates / Q000378:metabolism; D001419:Bacteria / Q000235:genetics / Q000378:metabolism*; D001673:Biodegradation, Environmental; D004583:Electrons; D005798:Genes, Bacterial / Q000235:genetics; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010472:Perchlorates / Q000302:isolation & purification* / Q000378:metabolism; D013431:Sulfates / Q000378:metabolism; D013997:Time Factors; D014865:Waste Disposal, Fluid / Q000379:methods*; D018508:Water Purification / Q000379:methods*
D000085:Acetates; D010472:Perchlorates; D013431:Sulfates; C494474:perchlorate