Putative innate immunity of antiatherogenic paraoxanase-2 via STAT5 signal transduction in HIV-1 infection of hematopoietic TF-1 cells and in SCID-hu mice.
Paraoxanase-2 (PON2) activity was increased upon HIV-1 infection of the CD34+CD4+ hematopoietic cell line TF-1. Thymocytes derived from the human fetal conjoint thymus/liver hematopoietic organ of SCID-hu mice also exhibited an increase in PON2 activity. Additionally, a remarkable increase of PON2 mRNA expression was also observed in both TF-1 and thymocytes following HIV-1 infection. The phosphorylation of STAT5 was decreased in TF-1 cells upon HIV-1 infection. Interestingly, phosphorylation of STAT5 does not occur in GM-CSF "starved" TF-1 cells; however, PON2 protein, activity and mRNA expression are increased under these conditions, similar to HIV-1 infection. We conclude that PON2 is induced in HIV-1 infection through a mechanism that may involve STAT5 inactivation.
Yuan
Jinyun
J
Laboratory of Stem Cell Biology, Torrey Pines Institute for Molecular Studies, 3550 General Atomics Court, San Diego, California 92121, USA.
Devarajan
Asokan
A
Moya-Castro
Rosita
R
Zhang
Menghua
M
Evans
Stella
S
Bourquard
Noam
N
Dias
Peter
P
Lacout
Catherine
C
Vainchenker
William
W
Reddy
Srinivasa T
ST
Koka
Prasad S
PS
D000818:Animals; D018952:Antigens, CD34 / Q000378:metabolism; D043303:Aryldialkylphosphatase / Q000235:genetics / Q000378:metabolism*; D050197:Atherosclerosis / Q000517:prevention & control; D015153:Blotting, Western; D002316:Cardiotonic Agents / Q000378:metabolism; D002478:Cells, Cultured; D005333:Fetus / Q000166:cytology; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D015658:HIV Infections / Q000276:immunology* / Q000378:metabolism / Q000821:virology; D015497:HIV-1 / Q000502:physiology*; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D006801:Humans; D007113:Immunity, Innate; D008099:Liver / Q000276:immunology / Q000821:virology; D051379:Mice; D016513:Mice, SCID; D010766:Phosphorylation; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D050799:STAT5 Transcription Factor / Q000037:antagonists & inhibitors / Q000378:metabolism*; D015398:Signal Transduction; D013950:Thymus Gland / Q000276:immunology / Q000821:virology
D018952:Antigens, CD34; D002316:Cardiotonic Agents; D012333:RNA, Messenger; D050799:STAT5 Transcription Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D043303:Aryldialkylphosphatase