Resveratrol reduces lipid peroxidation and increases sirtuin 1 expression in adult animals programmed by neonatal protein restriction.
Resveratrol (Res) has been associated with protective effects against oxidative stress. This study evaluated the effect of Res over lipid peroxidation, antioxidant defense, hepatic sirtuin 1 (SIRT1), which up-regulates antioxidant enzymes, and copper/zinc superoxide dismutase (Cu/Zn SOD) in adult offspring whose mothers were protein restricted during lactation. Lactating Wistar rats were divided into control (C) group, which were fed a normal diet (23% protein), and low-protein and high-carbohydrate (LPHC) group, which were fed a diet containing 8% protein. After weaning (21 days), C and LPHC offspring were fed a normal diet until they were 180 days old. At the 160th day, animals were separated into four groups as follows: control, control+Res, LPHC, and LPHC+Res. Resveratrol was given for 20 days (30  mg/kg per day by gavage). LPHC animals showed a higher total antioxidant capacity (TAC) without change in lipid peroxidation and SIRT1 expression. The treatment with Res increased TAC only in the control group without effect on lipid peroxidation and SIRT1. LPHC animals treated with Res had lower lipid peroxidation and higher protein and mRNA expression of SIRT1 without any further increase in TAC. No significant difference in liver Cu/Zn SOD expression was observed among the groups. In conclusion, maternal protein restriction during lactation programs the offspring for a higher antioxidant capacity, and these animals seem to respond to Res treatment with a lower lipid peroxidation and higher hepatic SIRT1 expression that we did not observe in the Res-treated controls. It is probable that the protective effect can be attributed to Res activating SIRT1, only in the LPHC-programmed group.
Franco
Juliana Gastão
JG
Department of Physiological Sciences, Roberto Alcântara Gomes Biology Institute, State University of Rio de Janeiro, Rio de Janeiro 20550-030, RJ, Brazil.
de Moura
Egberto Gaspar
EG
Koury
Josely Correa
JC
Trotta
Paula Affonso
PA
Cordeiro
Aline
A
Souza
Luana Lopes
LL
Almeida
Norma Aparecida dos Santos
NA
Lima
Natália da Silva
Nda S
Pazos-Moura
Carmen Cabanelas
CC
Lisboa
Patrícia Cristina
PC
Passos
Magna Cottini Fonseca
MC
D000818:Animals; D000831:Animals, Newborn; D000975:Antioxidants / Q000032:analysis / Q000494:pharmacology*; D001786:Blood Glucose / Q000187:drug effects; D018753:Diet, Protein-Restricted; D005260:Female; D007328:Insulin / Q000097:blood; D007333:Insulin Resistance / Q000502:physiology; D015227:Lipid Peroxidation / Q000187:drug effects*; D008055:Lipids / Q000097:blood; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D000077185:Resveratrol; D056564:Sirtuin 1 / Q000032:analysis / Q000378:metabolism*; D013267:Stilbenes / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000032:analysis
D000975:Antioxidants; D001786:Blood Glucose; D007328:Insulin; D008055:Lipids; D013267:Stilbenes; D013482:Superoxide Dismutase; D056564:Sirtuin 1; D000077185:Resveratrol