Exopolysaccharide activities from probiotic bifidobacterium: Immunomodulatory effects (on J774A.1 macrophages) and antimicrobial properties.
Exopolysaccharides (EPSs) are a metabolite of probiotics which have gained wide interest recently, but little is known about their function. EPS was isolated from Bifidobacterium longum BCRC 14634 and sterilized by 0.22 μm filter. The proliferation of J77A.1 macrophages and their secretion of the anti-inflammatory cytokine interleukin-10 (IL-10) was elevated after treatment with heat-killed B. longum or 5 μg/mL EPS. The endotoxin, lipopolysaccharides (LPS), a potent inducer of pro-inflammatory cytokine tumor necrosis factor-α (TNF-α), significantly suppressed the growth of J77A.1 cells, and induced the secretion of TNF-α from J774A.1 cells. Furthermore, 24h pretreatment with 5 μg/ml EPS suppressed 100 ng/ml LPS-induced cell growth inhibition and release of TNF-α from J774A.1 cells. Additional experiments showed that 80 μg/mL EPS had antimicrobial activity against 7 species of food-spoilage and infection bacteria. Our results suggest that EPS from B. longum might be useful as a mild immune modulator for macrophages, contributing to the capacity of B. longum to fight against gastrointestinal infections, and even some food-spoilage microbe.
Wu
Ming-Hsiu
MH
Department of Nutrition and Health Science, Fooyin University, Kaohsiung County, Taiwan.
Pan
Tzu-Ming
TM
Wu
Yu-Jen
YJ
Chang
Sue-Joan
SJ
Chang
Ming-Song
MS
Hu
Chun-Yi
CY
D000818:Animals; D000890:Anti-Infective Agents / Q000276:immunology / Q000378:metabolism / Q000494:pharmacology*; D001644:Bifidobacterium / Q000737:chemistry* / Q000276:immunology; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects; D007155:Immunologic Factors / Q000494:pharmacology*; D016753:Interleukin-10 / Q000276:immunology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology; D051379:Mice; D011135:Polysaccharides, Bacterial / Q000302:isolation & purification / Q000494:pharmacology*; D019936:Probiotics; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology
D000890:Anti-Infective Agents; D007155:Immunologic Factors; D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10