An expanding negative detection method for the visualization of DNA in polyacrylamide gels by eosin Y.
A sensitive and easy technique has been developed for the negative detection of DNA following PAGE using eosin Y. After electrophoresis, gels are fixed and stained within 40 min to provide a detection limit of 0.1-0.2 ng of single DNA band, which appears as transparent and colorless under the opaque gel matrix background. The sensitivity of the new stain is fourfold better than zinc-imidazole negative and ethidium bromide stains. Furthermore, the newly developed staining method has been successfully applied to RNA visualization in polyacrylamide gels. In addition, the inclusion of inorganic salts in staining solution was also investigated, which has great effect on the staining efficiency.
Zhu
Zhong-Xin
ZX
Zhejiang Provincial Key Laboratory of Biopharmaceuticals, Wenzhou Medical College, Wenzhou, Zhejiang, PR China.
Cong
Wei-Tao
WT
Zhang
Peng-Hui
PH
Ma
Wei-De
WD
Min
Liu
L
He
Hong-Zhang
HZ
Choi
Jung-Kap
JK
Jin
Li-Tai
LT
Li
Xiao-Kun
XK
D004247:DNA / Q000737:chemistry / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D004801:Eosine Yellowish-(YS) / Q000737:chemistry* / Q000494:pharmacology; D004996:Ethidium / Q000737:chemistry / Q000494:pharmacology; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000494:pharmacology; D007093:Imidazoles / Q000737:chemistry / Q000494:pharmacology; D016624:Negative Staining / Q000379:methods*; D012313:RNA / Q000737:chemistry / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D014170:Transformation, Genetic / Q000187:drug effects; D017967:Zinc Compounds / Q000737:chemistry / Q000494:pharmacology
D005456:Fluorescent Dyes; D007093:Imidazoles; D017967:Zinc Compounds; D012313:RNA; C029899:imidazole; D004247:DNA; D004996:Ethidium; D004801:Eosine Yellowish-(YS)