title: CX3CL1 expression induced by Candida albicans in oral fibroblasts.
abstract: Oral fibroblasts as well as keratinocytes are thought to influence host inflammatory responses against Candida albicans. However, little is known about chemokine expressions in oral fibroblasts against C. albicans infection. We therefore examined whether C. albicans induced several chemokines including fractalkine/CX3CL1 (CX3CL1), a unique chemokine that has properties of both chemoattractants and adhesion molecules, in fibroblasts and keratinocytes. The addition of C. albicans live cells to human immortalized oral keratinocytes (RT7) resulted in increases in the mRNA levels of multiple chemokines, but not of CX3CL1. In contrast, live and heat-killed C. albicans caused an increase in CX3CL1 mRNA and protein expression in human immortalized oral fibroblasts (GT1). CX3CL1 mRNA expression in GT1 cells was also enhanced by stimulation with a nonalbicans species of Candida. Further, the CX3CL1 chemokine domain showed antifungal activity against C. albicans. CX3CL1 secreted by oral fibroblasts appears to play an important role in the oral immune response to C. albicans infection.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Division of Cervico-Gnathostmatology, Programs for Applied Biomedicine, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan. otkouji@hiroshima-u.ac.jp
authors.forename: Kouji
authors.initials: K
authors.lastname: Ohta
authors.forename: Hiromi
authors.initials: H
authors.lastname: Nishi
authors.forename: Akiko
authors.initials: A
authors.lastname: Fukui
authors.forename: Hideo
authors.initials: H
authors.lastname: Shigeishi
authors.forename: Masaaki
authors.initials: M
authors.lastname: Takechi
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Kamata
mesh_terms: D000935:Antifungal Agents / Q000276:immunology / Q000494:pharmacology; D002176:Candida albicans / Q000276:immunology* / Q000378:metabolism; D041221:Candida glabrata / Q000276:immunology; D041022:Candida tropicalis / Q000276:immunology; D002180:Candidiasis, Oral / Q000276:immunology; D002460:Cell Line; D054428:Chemokine CX3CL1 / Q000276:immunology* / Q000378:metabolism* / Q000494:pharmacology; D018925:Chemokines / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D016207:Cytokines / Q000096:biosynthesis* / Q000378:metabolism; D004926:Escherichia coli / Q000276:immunology; D005347:Fibroblasts / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology; D006801:Humans; D007249:Inflammation / Q000276:immunology; D015603:Keratinocytes / Q000276:immunology / Q000378:metabolism; D009061:Mouth Mucosa / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology; D012333:RNA, Messenger / Q000235:genetics; D013211:Staphylococcus aureus / Q000276:immunology
chemical_list: D000935:Antifungal Agents; D054428:Chemokine CX3CL1; D018925:Chemokines; D016207:Cytokines; D012333:RNA, Messenger