title: Comparison of commercial ELISA blood tests for early pregnancy detection in dairy cows.
abstract: The objective of the present study was to compare two commercially available blood-based pregnancy tests, namely BioPRYN, an ELISA for pregnancy-specific protein B (PSPB), and an ELISA for pregnancy-associated glycoprotein (PAG), for early pregnancy diagnosis in dairy cattle using transrectal ultrasonography as a gold standard. Transrectal ultrasonography was conducted 26-58 days after artificial insemination (AI) in 197 cattle from 19 farms. Concurrently, a blood sample was collected for determination of serum PSPB and PAG. Transrectal palpation was performed approximately 120 days after AI to verify that pregnancy was maintained. For PSPB and PAG, there were no significant differences (P>0.05) in sensitivity (98.0 and 97.8%), specificity (97.1 and 91.2%), positive predictive values (99.3 and 97.8%), negative predictive values (91.9 and 91.2%) and accuracy (97.8 and 96.4%). In conclusion, the two blood pregnancy assays were equally efficacious and were highly accurate (based on transrectal ultrasonography as the gold standard).
authors.affiliation: Clinic for Cattle, University of Veterinary Medicine Hannover, Hannover, Germany. marion.piechotta@tiho-hannover.de
authors.forename: Marion
authors.initials: M
authors.lastname: Piechotta
authors.forename: Julia
authors.initials: J
authors.lastname: Bollwein
authors.forename: Morten
authors.initials: M
authors.lastname: Friedrich
authors.forename: Thomas
authors.initials: T
authors.lastname: Heilkenbrinker
authors.forename: Chuck
authors.initials: C
authors.lastname: Passavant
authors.forename: Josh
authors.initials: J
authors.lastname: Branen
authors.forename: Garth
authors.initials: G
authors.lastname: Sasser
authors.forename: Martina
authors.initials: M
authors.lastname: Hoedemaker
authors.forename: Heinrich
authors.initials: H
authors.lastname: Bollwein
mesh_terms: D000818:Animals; D001947:Breeding; D002417:Cattle; D003612:Dairying / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D006023:Glycoproteins / Q000097:blood; D007315:Insemination, Artificial / Q000662:veterinary; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000097:blood; D011259:Pregnancy Tests, Immunologic / Q000662:veterinary*; D011270:Pregnancy, Animal / Q000097:blood*; D011933:Reagent Kits, Diagnostic / Q000662:veterinary*; D012680:Sensitivity and Specificity
chemical_list: D006023:Glycoproteins; D011257:Pregnancy Proteins; D011933:Reagent Kits, Diagnostic