title: Droplet-based microfluidic platforms for single T cell secretion analysis of IL-10 cytokine.
abstract: Here we present a microfluidic method for the analysis of single cell secretions. The method co-encapsulates cells with microspheres conjugated with capture antibodies and detection fluorescence-labeled antibodies. The secreted substance captured on the microsphere surface and detected via detection antibodies generating a localized fluorescent signal on a microsphere surface. Using this method, CD4+CD25+ regulatory T cells were encapsulated and assayed to detect IL-10 secreting cell in population.
authors.affiliation: Center for Engineering in Medicine and Surgical Services, Massachusetts General Hospital, Harvard Medical School, 51 Blossom St., Boston, MA, USA. konry.tania@gmail.com
authors.forename: Tania
authors.initials: T
authors.lastname: Konry
authors.forename: Margarita
authors.initials: M
authors.lastname: Dominguez-Villar
authors.forename: Clare
authors.initials: C
authors.lastname: Baecher-Allan
authors.forename: David A
authors.initials: DA
authors.lastname: Hafler
authors.forename: Martin L
authors.initials: ML
authors.lastname: Yarmush
mesh_terms: D002469:Cell Separation / Q000295:instrumentation*; D002478:Cells, Cultured; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D016753:Interleukin-10 / Q000378:metabolism*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D013601:T-Lymphocytes / Q000378:metabolism*
chemical_list: D016753:Interleukin-10