title: Total chemical synthesis of human proinsulin.
abstract: A convergent synthetic strategy based on modern chemical ligation methods was used to make human proinsulin. The synthetic protein was characterized by LCMS, CD spectroscopy, and by 1D- and 2D-NMR spectroscopy. Synthetic human proinsulin had full biochemical activity in a receptor-binding assay.
authors.affiliation: Department of Chemistry, Institute for Biophysical Dynamics, The University of Chicago, Chicago, Illinois 60637, USA.
authors.forename: Samuel
authors.initials: S
authors.lastname: Luisier
authors.forename: Michal
authors.initials: M
authors.lastname: Avital-Shmilovici
authors.forename: Michael A
authors.initials: MA
authors.lastname: Weiss
authors.forename: Stephen B H
authors.initials: SB
authors.lastname: Kent
mesh_terms: D000595:Amino Acid Sequence; D002942:Circular Dichroism; D006801:Humans; D008969:Molecular Sequence Data; D011384:Proinsulin / Q000138:chemical synthesis* / Q000737:chemistry; D013237:Stereoisomerism
chemical_list: D011384:Proinsulin