title: Biodegradability and toxicity assessment of bleach plant effluents treated anaerobically.
abstract: As part of an experimental project on the treatment of bleach plant effluents the results of biodegradability and toxicity assessment of effluents from a bench-scale horizontal anaerobic immobilized bioreactor (HAIB) are discussed in this paper. The biodegradability of the bleach plant effluents from a Kraft pulp mill treated in the HAIB was evaluated using the modified Zahn-Wellens test. The inoculum came from a pulp mill wastewater treatment plant and the dissolved organic carbon (DOC) was used as the indicator of organic matter removal. The acute and chronic toxicity removal during the anaerobic treatment was estimated using Daphnia similis and Ceriodaphnia silvestrii respectively. Moreover, the evaluation of chromosome aberrations (CA), micronucleus frequencies (MN) and mitotic index (IM) in Allium cepa cells were used as genotoxicity indicators. The results indicate that the effluents from the anaerobic reactor are amenable to aerobic polishing. Acute and chronic toxicity were reduced by 90 and 81%, respectively. The largest CA and MN incidence in the meristematic cells of A. cepa were observed after exposure to the raw bleach plant effluent. The HAIB was able to reduce the acute and chronic toxicity as well as chromosome aberrations and the occurrence of micronucleus.
authors.affiliation: Department of Hydraulic and Sanitary Engineering, Sao Carlos School of Engineering, University of Sao Paulo, Av Trabalhador São-carlense 400, Sao Carlos SP, Brazil. tatianna@sc.usp.br
authors.forename: T R
authors.initials: TR
authors.lastname: Chaparro
authors.forename: C M
authors.initials: CM
authors.lastname: Botta
authors.forename: E C
authors.initials: EC
authors.lastname: Pires
mesh_terms: D000332:Aerobiosis; D000693:Anaerobiosis; D000818:Animals; D001673:Biodegradation, Environmental; D019149:Bioreactors / Q000382:microbiology*; D057886:Bleaching Agents / Q000032:analysis / Q000633:toxicity; D003621:Daphnia / Q000187:drug effects / Q000254:growth & development; D006843:Hydrocarbons, Chlorinated / Q000032:analysis / Q000633:toxicity; D048629:Micronuclei, Chromosome-Defective / Q000139:chemically induced; D019697:Onions / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D012639:Seeds / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D023382:Toxicity Tests, Acute / Q000379:methods; D036401:Toxicity Tests, Chronic / Q000379:methods; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000633:toxicity; D018508:Water Purification / Q000295:instrumentation / Q000379:methods*
chemical_list: D057886:Bleaching Agents; D006843:Hydrocarbons, Chlorinated; D014874:Water Pollutants, Chemical