title: [Structural characterization of a glucan isolated from Gastrodia elata].
abstract: OBJECTIVE
  To study a glucan (GB II) isolated from Gastrodia elata.
  
  
  METHODS
  The glucan was obtained with water extraction, alcohol precipitate, DEAE-Sepharose Fast Flow column and Sepharose CL-6B column chromatography; sugar composition analysis, IR and NMR were used to determine the structural feature.
  
  
  RESULTS
  The molecular weight of the glucan was 4 300 dalton estimated by HPGPC; it contained 27 glucose residues, which mainly composed of alpha-D-(1-->4)-glucose with little glucuronic acid and branch O-6 points.
  
  
  CONCLUSIONS
  The glucan was a new glucan for the first report from Gastrodia elata.
authors.affiliation: Shanghai University of Traditional Chinese Medicine, Institute of Chinese Materia Medica, Shanghai 201210, China.
authors.forename: Qi-ming
authors.initials: QM
authors.lastname: Hong
authors.forename: Song-shan
authors.initials: SS
authors.lastname: Shi
authors.forename: Cai
authors.initials: C
authors.lastname: Wang
authors.forename: Shun-chun
authors.initials: SC
authors.lastname: Wang
authors.forename: Xi-lin
authors.initials: XL
authors.lastname: Li
authors.forename: Xiu-jia
authors.initials: XJ
authors.lastname: Zhou
mesh_terms: D008401:Gas Chromatography-Mass Spectrometry; D031668:Gastrodia / Q000737:chemistry*; D005936:Glucans / Q000737:chemistry* / Q000302:isolation & purification; D020723:Glucuronic Acid / Q000032:analysis* / Q000302:isolation & purification; D008745:Methylation; D015394:Molecular Structure; D008970:Molecular Weight; D009005:Monosaccharides / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D035281:Plant Tubers / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D013055:Spectrophotometry, Infrared
chemical_list: D005936:Glucans; D009005:Monosaccharides; D020723:Glucuronic Acid