title: Inhibitors of nitric oxide production from Stemona javanica.
abstract: In our screening program for bioactive natural products from our library of tropical plants, the extract prepared from the roots of Stemona javanica inhibited NO production in mouse macrophage-like cell line J774.1 stimulated by lipopolysaccharide (LPS). Bioassay-guided fractionation of the extract from S. javanica led to the isolation of two active compounds, stemofoline (1) and stemanthrene C (2). The inhibition mechanism of 1 was proposed to suppress iNOS expression in J774.1 cells stimulated by LPS, whereas that of 2 was due to potent radical scavenging activity resulting in NO inhibitory activity.
authors.affiliation: Faculty of Pharmaceutical Sciences, Hoshi University, Shinagawa, Tokyo, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Hosoya
authors.forename: Fumie
authors.initials: F
authors.lastname: Yamasaki
authors.forename: Asami
authors.initials: A
authors.lastname: Nakata
authors.forename: Abdul
authors.initials: A
authors.lastname: Rahman
authors.forename: Idha
authors.initials: I
authors.lastname: Kusumawati
authors.forename: Noor Cholies
authors.initials: NC
authors.lastname: Zaini
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Morita
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D002460:Cell Line; D006576:Heterocyclic Compounds, 4 or More Rings / Q000302:isolation & purification / Q000494:pharmacology*; D008070:Lipopolysaccharides; D008264:Macrophages / Q000187:drug effects*; D051379:Mice; D009569:Nitric Oxide / Q000096:biosynthesis*; D052247:Nitric Oxide Synthase Type II / Q000037:antagonists & inhibitors*; D010616:Phenanthrenes / Q000302:isolation & purification / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018517:Plant Roots; D041901:Stemonaceae / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D006576:Heterocyclic Compounds, 4 or More Rings; D008070:Lipopolysaccharides; D010616:Phenanthrenes; D010936:Plant Extracts; C561211:stemanthrene C; C430220:stemofoline; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II