NHANES monitoring of serum 25-hydroxyvitamin D: a roundtable summary.
A roundtable to discuss monitoring of serum 25-hydroxyvitamin D [25(OH)D] in the NHANES was held in late July 2009. Topics included the following: 1) options for dealing with assay fluctuations in serum 25(OH)D in the NHANES conducted between 1988 and 2006; 2) approaches for transitioning between the RIA used in the NHANES between 1988 and 2006 to the liquid chromatography tandem MS (LC-MS/MS) measurement procedure to be used in NHANES 2007 and later; 3) approaches for integrating the recently available standard reference material for vitamin D in human serum (SRM 972) from the National Institute of Standards and Technology (NIST) into the NHANES; 4) questions regarding whether the C-3 epimer of 25-hydroxyvitamin D3 [3-epi-25(OH)D3] should be measured in NHANES 2007 and later; and 5) identification of research and educational needs. The roundtable experts agreed that the NHANES data needed to be adjusted to control for assay fluctuations and offered several options for addressing this issue. The experts suggested that the LC-MS/MS measurement procedure developed by NIST could serve as a higher order reference measurement procedure. They noted the need for a commutability study for the recently released NIST SRM 972 across a range of measurement procedures. They suggested that federal agencies and professional organizations work with manufacturers to improve the quality and comparability of measurement procedures across all laboratories. The experts noted the preliminary nature of the evidence of the 3-epi-25(OH)D3 but felt that it should be measured in 2007 NHANES and later.
Yetley
Elizabeth A
EA
Office of Dietary Supplements, National Institutes of Health, Bethesda, MD 20892, USA. beth@yetley.com
Pfeiffer
Christine M
CM
Schleicher
Rosemary L
RL
Phinney
Karen W
KW
Lacher
David A
DA
Christakos
Sylvia
S
Eckfeldt
John H
JH
Fleet
James C
JC
Howard
George
G
Hoofnagle
Andrew N
AN
Hui
Siu L
SL
Lensmeyer
Gary L
GL
Massaro
Joseph
J
Peacock
Munro
M
Rosner
Bernard
B
Wiebe
Donald
D
Bailey
Regan L
RL
Coates
Paul M
PM
Looker
Anne C
AC
Sempos
Christopher
C
Johnson
Clifford L
CL
Picciano
Mary Frances
MF
D015652:25-Hydroxyvitamin D 2 / Q000031:analogs & derivatives / Q000097:blood* / Q000737:chemistry / Q000592:standards; D002112:Calcifediol / Q000097:blood* / Q000737:chemistry / Q000592:standards; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D009749:Nutrition Surveys; D012015:Reference Standards; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry
D015652:25-Hydroxyvitamin D 2; D002112:Calcifediol