title: α-Tocopherol adipose tissue stores are depleted after burn injury in pediatric patients.
abstract: BACKGROUND
  We previously showed that thermal injury depletes plasma vitamin E in pediatric burn patients; however, plasma changes may reflect immediate alterations in vitamin E nutriture. Adipose tissue α-tocopherol concentrations are generally accepted to reflect long-term vitamin E status.
  
  
  OBJECTIVE
  To test the hypothesis that thermal injury depletes body stores of vitamin E, α-tocopherol concentrations were measured in adipose tissue samples.
  
  
  METHODS
  Pediatric patients (n = 8) were followed up to 1 y after burn injury. Surgically obtained samples were collected at various intervals and stored at -80°C in a biorepository. α- and γ-Tocopherols, cholesterol, and triglycerides were measured in the same tissue aliquot.
  
  
  RESULTS
  During the first week after injury, adipose tissue α-tocopherol concentrations were within the expected normal range of 199 ± 40 nmol/g adipose tissue but were substantially lower at weeks 2 and 3 (133 ± 13 and 109 ± 8 nmol/g adipose tissue, respectively). Individual rates of decrease, estimated by linear regression, showed that adipose tissue α-tocopherol decreased by an average of 6.1 ± 0.6 nmol/g daily. During the first month after injury, adipose tissue triglyceride concentrations also decreased, whereas no changes in cholesterol concentrations occurred.
  
  
  CONCLUSIONS
  These data emphasize that the burn injury experienced by these pediatric patients altered their metabolism such that vitamin E status diminished during the month after injury. Further studies are needed to evaluate the mechanism and consequences of the observed vitamin E depletion. This trial was registered at clinicaltrials.gov as NCT00675714.
authors.affiliation: Linus Pauling Institute, Oregon State University, Corvallis, OR 97331-6512, USA. maret.traber@oregonstate.edu
authors.forename: Maret G
authors.initials: MG
authors.lastname: Traber
authors.forename: Scott W
authors.initials: SW
authors.lastname: Leonard
authors.forename: Daniel L
authors.initials: DL
authors.lastname: Traber
authors.forename: Lillian D
authors.initials: LD
authors.lastname: Traber
authors.forename: James
authors.initials: J
authors.lastname: Gallagher
authors.forename: Gerd
authors.initials: G
authors.lastname: Bobe
authors.forename: Marc G
authors.initials: MG
authors.lastname: Jeschke
authors.forename: Celeste C
authors.initials: CC
authors.lastname: Finnerty
authors.forename: David
authors.initials: D
authors.lastname: Herndon
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D000293:Adolescent; D002056:Burns / Q000150:complications / Q000378:metabolism*; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D008297:Male; D012016:Reference Values; D012044:Regression Analysis; D014280:Triglycerides / Q000378:metabolism*; D014811:Vitamin E Deficiency / Q000209:etiology* / Q000378:metabolism; D024502:alpha-Tocopherol / Q000378:metabolism*
chemical_list: D014280:Triglycerides; D024502:alpha-Tocopherol