Simultaneous detection of attomolar pathogen DNAs by Bio-MassCode mass spectrometry.
A general method is described to analyze pathogen DNAs in homogenous solution by a novel Bio-MassCode probe, using a small organic (namely disulfide) "reporter mass" that self-assembled in oligonucleotide-modified gold nanoparticles. With conventional MALDI TOF MS, the assay simultaneously detected DNAs for HIV, HBV, HCV, and TP with extraordinarily high sensitivity in the low attomolar (10(-18) M) range without any other amplification.
Yang
Bing
B
Institute of Molecular Medicine and School of Medicine, Nanjing University, Nanjing 210093, China.
Gu
Kai
K
Sun
Xiaoru
X
Huang
Huan
H
Ding
Yibing
Y
Wang
Fuqiang
F
Zhou
Guohua
G
Huang
Lequn Lee
LL
D004279:DNA, Viral / Q000032:analysis*; D006046:Gold / Q000737:chemistry; D006678:HIV / Q000235:genetics; D016174:Hepacivirus / Q000235:genetics; D006515:Hepatitis B virus / Q000235:genetics; D053768:Metal Nanoparticles / Q000737:chemistry; D009841:Oligonucleotides / Q000737:chemistry; D012367:RNA, Viral / Q000032:analysis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D004279:DNA, Viral; D009841:Oligonucleotides; D012367:RNA, Viral; D006046:Gold