title: A molecular approach for identification of paramphistomes from Africa and Asia.
abstract: Paramphistomiasis is a disease of domestic and wild ruminants caused by some digenetic trematodes of the superfamily Paramphistomoidea. Human infections with certain species of paramphistomes have been reported. Given their ubiquity and their abundance within hosts, it seems likely that the importance of these worms is underestimated globally. Based on the reported difficulties of morphological identification of paramphistomes, the development of alternative approaches to better delineate species is needed. Paramphistome samples examined during this study were cercariae, rediae from snail hosts and adult flukes obtained from slaughterhouses from Kenya, Tanzania, Egypt and Nepal. The results confirm that ITS2 is a good molecular marker for paramphistome identification and can be used to determine the affiliation of samples among the different taxa of Paramphistomoidea. Our 22 samples include eight species of paramphistomes. Ten samples of paramphistome larvae (cercariae or rediae) yielded seven species and the 12 samples of adult flukes from bovines yielded four, only one of which was not also represented among the larvae. We found three cases in which the sequences for adult worms and larvae matched, thus revealing the hosts involved locally in those particular life cycles. We interpret our specimens to include five species from Kenya, two from Nepal, two from Tanzania and one from Egypt. Bulinus forskalii was found infected with three species of paramphistomes. The results of the present study will help construct a framework to facilitate identification and eventually control of this underestimated cosmopolitan group of digeneans.
authors.affiliation: Parasitology Department, Medical Research Institute, Alexandria University, 165 El-Horreya Avenue, El-Hadara, Alexandria 21561, Egypt. waelotfy@alex-mri.edu.eg
authors.forename: Wael M
authors.initials: WM
authors.lastname: Lotfy
authors.forename: Sara V
authors.initials: SV
authors.lastname: Brant
authors.forename: Karam I
authors.initials: KI
authors.lastname: Ashmawy
authors.forename: Ramesh
authors.initials: R
authors.lastname: Devkota
authors.forename: Gerald M
authors.initials: GM
authors.lastname: Mkoji
authors.forename: Eric S
authors.initials: ES
authors.lastname: Loker
mesh_terms: D000349:Africa; D000818:Animals; D001208:Asia; D018509:DNA, Helminth / Q000235:genetics; D021901:DNA, Intergenic / Q000235:genetics; D010250:Paramphistomatidae / Q000145:classification*; D058974:Phylogeography
chemical_list: D018509:DNA, Helminth; D021901:DNA, Intergenic