title: In vivo acid etching effect on bacteria within caries-affected dentin.
abstract: Acid etching procedures may disrupt residual bacteria and contribute to the success of incomplete caries removal followed by adhesive restoration. This study evaluated the in vivo effect of acid etching on cariogenic bacterial activity within affected dentin after minimally invasive treatment of caries lesions. Twenty-eight carious permanent teeth received standardized selective caries removal and random acid etch treatment (E) or not (NE) prior to adhesive restoration. Baseline and 3-month dentin biopsies were collected. The number of bacteria and activity of total bacterial cells and Streptococcus mutans were determined by quantitative PCR and RT-PCR. No statistically significant differences were observed in total bacterial number and activity between E and NE treatments (p > 0.3008). For NE, however, the residual S. mutans bacterial cells were reduced (p = 0.0027), while the activity per cell was significantly increased (p = 0.0010) after reentry at 3 months after restoration. This effect was not observed in group E. Although no significant differences were found between groups, this study suggests that acid etching of affected dentin prior to adhesive restoration may directly or indirectly have an inhibitive effect on the activity of residual cariogenic bacteria. Further research is required to investigate this potential effect.
authors.affiliation: School of Dentistry, University of Michigan, Ann Arbor, MI 48109, USA.
authors.forename: F
authors.initials: F
authors.lastname: Gu
authors.forename: E
authors.initials: E
authors.lastname: Bresciani
authors.forename: T J
authors.initials: TJ
authors.lastname: Barata
authors.forename: T C
authors.initials: TC
authors.lastname: Fagundes
authors.forename: M F
authors.initials: MF
authors.lastname: Navarro
authors.forename: S H
authors.initials: SH
authors.lastname: Dickens
authors.forename: J C
authors.initials: JC
authors.lastname: Fenno
authors.forename: M C
authors.initials: MC
authors.lastname: Peters
mesh_terms: D000134:Acid Etching, Dental / Q000379:methods*; D000328:Adult; D001419:Bacteria / Q000187:drug effects* / Q000302:isolation & purification; D058491:Bacterial Load; D001706:Biopsy; D003188:Composite Resins / Q000737:chemistry; D004269:DNA, Bacterial / Q000032:analysis; D057087:Dental Atraumatic Restorative Treatment / Q000379:methods; D001840:Dental Bonding; D003731:Dental Caries / Q000382:microbiology* / Q000628:therapy; D003764:Dental Materials / Q000737:chemistry / Q000494:pharmacology*; D003804:Dentin / Q000382:microbiology*; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008297:Male; D010756:Phosphoric Acids / Q000494:pharmacology*; D013295:Streptococcus mutans / Q000187:drug effects / Q000302:isolation & purification
chemical_list: D003188:Composite Resins; D004269:DNA, Bacterial; D003764:Dental Materials; C097956:Epic TMPT; D010756:Phosphoric Acids; C030242:phosphoric acid