title: [Study on antioxidant effect of Herba Lycopi and screening of active fractions].
abstract: OBJECTIVE
  To Study the antioxidant activity of Herba Lycopi, and screen the main active fractions.
  
  
  METHODS
  The different polar fractions were isolated from the ethanol extract of Herba Lycopi. The scavenging activity to DPPH and superoxide anions, reducing effect on Fe3+, and inhibiting activity of lipid peroxidation of the fractions were studied in vitro by high-throughput screening technique.
  
  
  RESULTS
  The ethanol extract, ethyl acetate fraction (LLE-A. E, MCCM2921) and n-buthanol fraction (LLE-A. B, MCCM2922) of Herba Lycopi showed the scavenging activity to DPPH and superoxide anions, reducing effect on Fe3+, and inhibiting activity of lipid peroxidation.
  
  
  CONCLUSIONS
  The ethanol extract and different polar fractions showed antioxidant capacity in vitro. That may be explain that the effect of activating blood and dissolving stasis and anti-fibrosis of Herba Lycopi in traditional application. The results may be helpful for further development of Herba Lycopi as a low-toxic antioxidant drug.
authors.affiliation: Key Laboratory of Chinese Internal Medicine Ministry of Education and Beijing City, Dongzhimen Hospital Affiliated to Bejing University of Chinese Medicine, Beijing 100700, China. nieboww_1977@163.com
authors.forename: Bo
authors.initials: B
authors.lastname: Nie
authors.forename: Feng
authors.initials: F
authors.lastname: Huang
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
authors.forename: Guanhua
authors.initials: G
authors.lastname: Du
authors.forename: Xinmiao
authors.initials: X
authors.lastname: Liang
authors.forename: Peigen
authors.initials: P
authors.lastname: Xiao
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000032:analysis* / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects; D031333:Lycopus / Q000737:chemistry*; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism; D010936:Plant Extracts / Q000032:analysis* / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000975:Antioxidants; D010936:Plant Extracts