Serotonin receptor 5-HT5A in rat hippocampus decrease by leptin treatment.
5-Hydroxytryptamine (5-HT) is involved in a variety of different physiological processes and behaviors through the activation of equally diverse receptors subtypes. In this work we studied the changes on the expression of 5-HT(5A) receptors in rat hippocampus induced by leptin, an adipocyte-derived hormone that has been reported to participate in the modulation of food intake and in adult hippocampal neurogenesis. To study the effect of leptin on the 5-HT(5A) receptor gene expression a qRT-PCR was used and the distribution of those receptors in the hippocampus was visualized by immunohistochemistry. Rats were separated in four groups: control (untreated rats), leptin-treated, serotonin-treated and leptin+serotonin treated. The results showed that even though the 5-HT(5A) gene expression did not change in the hippocampus of any of the treated groups, in the rats treated with leptin and serotonin, the specific immunostaining for the 5-HT(5A) serotonin receptor decreased significantly in the dentate gyrus.
García-Alcocer
Guadalupe
G
Facultad de Química, Universidad Autónoma de Querétaro, Centro Universitario, Querétaro 76010, Mexico. leguga@email.com
Rodríguez
Angelina
A
Moreno-Layseca
Paulina
P
Berumen
Laura C
LC
Escobar
Jesica
J
Miledi
Ricardo
R
D000818:Animals; D018891:Dentate Gyrus / Q000378:metabolism; D015536:Down-Regulation / Q000502:physiology*; D005786:Gene Expression Regulation / Q000502:physiology; D006624:Hippocampus / Q000378:metabolism*; D007274:Injections, Intraperitoneal; D020738:Leptin / Q000008:administration & dosage / Q000502:physiology*; D008297:Male; D055495:Neurogenesis / Q000235:genetics / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D011985:Receptors, Serotonin / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D012701:Serotonin / Q000008:administration & dosage / Q000502:physiology*; D009435:Synaptic Transmission / Q000502:physiology
D020738:Leptin; D011985:Receptors, Serotonin; C078730:serotonin 5 receptor; D012701:Serotonin