Small ncRNA expression and regulation under hypoxia in neural progenitor cells.
Small non-coding RNA (ncRNA) plays critical roles in a large number of cellular processes, including neural development, cell survival and cell determination. Our previous work showed that low oxygen promoted the survival and proliferation of neural progenitor cells (NPCs) in vitro. In this study, we examine the expression and regulation of small ncRNAs in the hypoxia-driven proliferation of NPCs. The expression profiles of ncRNAs in NPCs under hypoxia were detected using microarray analysis. Results of significance analysis of microarrays (SAM) revealed that 15 small RNAs were up-regulated at least threefold and 11 were down-regulated under hypoxic conditions. The differentially expressed small ncRNAs were confirmed by quantitative RT-PCR, and miR-210 was observed to be highly expressed in NPCs under hypoxic conditions. Further study showed that hypoxia-inducible factor (HIF)-1α had a direct impact on the putative promoter regions of miR-210. From these results, we conclude that some small ncRNAs participate in the regulation of the proliferation of NPCs under hypoxia and that miR-210 is directly regulated by HIF-1α.
Liu
Zhao-hui
ZH
Department of Brain Protection and Plasticity, Institute of Basic Medical Sciences, Beijing, People's Republic of China.
Yang
Guang
G
Zhao
Tong
T
Cao
Guo-jun
GJ
Xiong
Lei
L
Xia
Wei
W
Huang
Xin
X
Wu
Li-ying
LY
Wu
Kuiwu
K
Fan
Ming
M
Shao
Ning-sheng
NS
Zhu
Ling-ling
LL
D000818:Animals; D015687:Cell Hypoxia / Q000235:genetics; D002478:Cells, Cultured; D004622:Embryo, Mammalian; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D046228:Microarray Analysis; D058953:Neural Stem Cells / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology*; D009474:Neurons / Q000378:metabolism / Q000502:physiology; D010100:Oxygen / Q000494:pharmacology; D058727:RNA, Small Untranslated / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D013234:Stem Cells / Q000378:metabolism / Q000502:physiology; D054928:Validation Studies as Topic
D058727:RNA, Small Untranslated; D010100:Oxygen