title: Comprehensive proteomics.
abstract: Extensive proteome discovery projects using a variety of mass spectrometric techniques have identified proteins matching to 50-70% of the predicted gene models of various species. Comprehensive proteome coverage is desirable for the unbiased comparison of protein quantities between different biological states and for the meaningful comparison of data from multiple samples. Here we discuss the feasibility of this goal in the light of recent technological developments.
authors.affiliation: European Molecular Biology Laboratory, Meyerhofstrasse 1, Heidelberg, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Beck
authors.forename: Manfred
authors.initials: M
authors.lastname: Claassen
authors.forename: Ruedi
authors.initials: R
authors.lastname: Aebersold
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods; D015870:Gene Expression; D006801:Humans; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods; D008432:Mathematical Computing; D008954:Models, Biological; D010446:Peptide Fragments / Q000032:analysis*; D011506:Proteins / Q000737:chemistry / Q000235:genetics; D020543:Proteome / Q000032:analysis* / Q000737:chemistry; D040901:Proteomics / Q000379:methods
chemical_list: D010446:Peptide Fragments; D011506:Proteins; D020543:Proteome