title: In situ detection of urease-positive Helicobacter pylori-like organisms on swine gastric mucosa.
abstract: The objective of this study was to improve the visual localization of urease activity of Helicobacter pylori-like organisms (HPLO) on swine gastric mucosa by in vitro optimization of the urea concentration and pH indicator of a urease test reagent. Five 21-day-old conventional pigs were infected orally with HPLO (3 pigs) or Brucella broth alone (2 pigs). At 17 d after infection the pigs were euthanized and their stomachs excised and tested for HPLO by a modified urease test formulation sprayed onto the gastric mucosa, as well as confirmatory culture and isolation of HPLO from urease-positive sites. This study showed improved detection of HPLO in porcine gastric mucosa with the use of a modified urease test formulation containing 5% urea and the pH indicator bromocresol purple compared with the use of a conventional formulation of 2% urea and phenol red. This test can readily be applied to achieve a presumptive diagnosis of HPLO in cases of gastritis or gastric esophageal ulceration in pigs.
authors.affiliation: Department of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan. kathy.mcintosh@usask.ca
authors.forename: Kathleen A
authors.initials: KA
authors.lastname: McIntosh
authors.forename: Steven
authors.initials: S
authors.lastname: Krakowka
authors.forename: Susan S
authors.initials: SS
authors.lastname: Ringler
authors.forename: John A
authors.initials: JA
authors.lastname: Ellis
mesh_terms: D000818:Animals; D005753:Gastric Mucosa / Q000382:microbiology*; D016998:Helicobacter / Q000145:classification / Q000302:isolation & purification*; D016481:Helicobacter Infections / Q000382:microbiology / Q000662:veterinary*; D013045:Species Specificity; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*; D014510:Urease / Q000378:metabolism*
chemical_list: D014510:Urease