title: Scraping and stapling of end-grafted DNA chains by a bioadhesive spreading vesicle to reveal chain internal friction and topological complexity.
abstract: Stained end-grafted DNA molecules about 20 μm long are scraped away and stretched out by the spreading front of a bioadhesive vesicle. Tethered biotin ligands bind the vesicle bilayer to a streptavidin substrate, stapling the DNAs into frozen confinement paths. Image analysis of the stapled DNA gives access, within optical resolution, to the local stretching values of individual DNA molecules swept by the spreading front, and provides evidence of self-entanglements.
authors.affiliation: Institute of Fundamental Physics, Department of Physics, Sejong University, Seoul 143-743, Korea.
authors.forename: Gimoon
authors.initials: G
authors.lastname: Nam
authors.forename: Marie Laure
authors.initials: ML
authors.lastname: Hisette
authors.forename: Yuting Liang
authors.initials: YL
authors.lastname: Sun
authors.forename: Thomas
authors.initials: T
authors.lastname: Gisler
authors.forename: Albert
authors.initials: A
authors.lastname: Johner
authors.forename: Fabrice
authors.initials: F
authors.lastname: Thalmann
authors.forename: André Pierre
authors.initials: AP
authors.lastname: Schröder
authors.forename: Carlos Manuel
authors.initials: CM
authors.lastname: Marques
authors.forename: Nam-Kyung
authors.initials: NK
authors.lastname: Lee
mesh_terms: D000269:Adhesives / Q000737:chemistry*; D000818:Animals; D040761:Biomimetic Materials / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D017276:Friction; D021621:Imaging, Three-Dimensional; D009690:Nucleic Acid Conformation; D053835:Unilamellar Liposomes / Q000737:chemistry*
chemical_list: D000269:Adhesives; D053835:Unilamellar Liposomes; D004247:DNA