Isolation of genomic DNA from medicinal plants without liquid nitrogen.
Genomic DNA was extracted from eight medicinal plants using the present DNA extraction protocols (CTAB extraction method) with some modifications. Leaves were fixed in different fixing solutions containing absolute alcohol (99.99%), chloroform and EDTA, but without liquid nitrogen. DNA quality and quantity obtained were comparable to those isolated with liquid nitrogen, as the lambda260/lambda280 ratio with liquid nitrogen was in range 1.3-1.7 and with other fixing solutions it was 1.1-1.5. Absolute alcohol showed best results as fixing solution. Good quality of DNA was isolated without using liquid nitrogen from different medicinal plant species. DNA isolated by this method was suitable for various molecular biology applications.
Sharma
Pratibha
P
Department of Bioscience & Biotechnology, Banasthali University, Banasthali 304 022, India.
Joshi
Neha
N
Sharma
Anubhuti
A
D018744:DNA, Plant / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D005615:Freezing; D018745:Genome, Plant; D018515:Plant Leaves / Q000737:chemistry / Q000235:genetics*; D010946:Plants, Medicinal / Q000737:chemistry / Q000235:genetics*; D016133:Polymerase Chain Reaction
D018744:DNA, Plant