title: [Kinetics of interaction between apomyoglobin and phospholipid membrane].
abstract: The interaction of apomyoglobin and its mutant forms with phospholipid membranes was studied using tryptophan fluorescence and CD in the far UV-region. It is shown that a negatively charged phospholipid membrane can have a double effect on the structure of protein molecule upon their interaction: it denatures the native structure of the protein to its intermediate state similar to that in solution, acting as a moderately denaturing reagent. On the other hand, it can structure the unfolded protein to the same intermediate state stabilizing its structure. The kinetics of interaction between the protein and its mutant forms and the phospholipid membrane depends on the charge of the membrane surface. Here the rate of this interaction depends on the phospholipids vesicle concentration and the protein molecule stability increasing with a decrease of the latter. The importance of the obtained results for the folding of membrane proteins and the choice of the pathway for target delivery of protein drugs are discussed.
authors.forename: V A
authors.initials: VA
authors.lastname: Balobanov
authors.forename: N B
authors.initials: NB
authors.lastname: Il'ina
authors.forename: N S
authors.initials: NS
authors.lastname: Katina
authors.forename: I A
authors.initials: IA
authors.lastname: Kashparov
authors.forename: D A
authors.initials: DA
authors.lastname: Dolgikh
authors.forename: V E
authors.initials: VE
authors.lastname: Bychkova
mesh_terms: D001059:Apoproteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D002942:Circular Dichroism; D006801:Humans; D007700:Kinetics; D008567:Membranes, Artificial; D009154:Mutation; D009211:Myoglobin / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D010743:Phospholipids / Q000737:chemistry* / Q000378:metabolism; D017510:Protein Folding; D055550:Protein Stability; D013050:Spectrometry, Fluorescence
chemical_list: D001059:Apoproteins; D008567:Membranes, Artificial; D009211:Myoglobin; D010743:Phospholipids; C010857:apomyoglobin