title: Redetermination of the X-ray structure of nitroxylcobalamin: base-on nitroxylcobalamin exhibits a remarkably long Co-N(dimethylbenzimidazole) bond distance.
abstract: The X-ray structures of three new crystals of nitroxylcobalamin (NOCbl) have been determined. Unlike our earlier reported structure in which NOCbl was partially oxidized (L. Hannibal, C. A. Smith, D. W. Jacobsen and N. E. Brasch, Angew. Chem., Int. Ed. 2007, 46, 5140), the O atom of the nitroxyl ligand is located in a single position with a N=O bond distance of 1.12-1.14 Å, consistent with a double bond. The Co-N-O angle is in the 118.9-120.3 Å range. The α-axial Co-N(dimethylbenzimidazole) (Co-NB3) bond distance is a remarkable 2.32-2.35 Å in length, ~0.1 Å longer than that reported for all other cobalamin structures. The change in the Gibbs free energy for the base-on/base-off equilibrium now correlates extremely well with the Co-NB3 bond distance, as observed for other cobalamins.
authors.affiliation: Department of Chemistry, Kent State University, Kent, OH 44242, USA.
authors.forename: Hanaa A
authors.initials: HA
authors.lastname: Hassanin
authors.forename: Mohamed F
authors.initials: MF
authors.lastname: El-Shahat
authors.forename: Serena
authors.initials: S
authors.lastname: DeBeer
authors.forename: Clyde A
authors.initials: CA
authors.lastname: Smith
authors.forename: Nicola E
authors.initials: NE
authors.lastname: Brasch
mesh_terms: D001562:Benzimidazoles / Q000737:chemistry*; D003035:Cobalt / Q000737:chemistry*; D009584:Nitrogen / Q000737:chemistry*; D009589:Nitrogen Oxides / Q000737:chemistry*; D009942:Organometallic Compounds / Q000737:chemistry*; D053838:Scattering, Small Angle; D014805:Vitamin B 12 / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D001562:Benzimidazoles; D009589:Nitrogen Oxides; D009942:Organometallic Compounds; D003035:Cobalt; C015158:5,6-dimethylbenzimidazole; C039900:nitroxyl; D009584:Nitrogen; D014805:Vitamin B 12