Transmission electron microscopy of cartilage and bone.
This review of cartilage microscopy is restricted to a brief description of the major molecular components in cartilage and bone and an in-depth discussion of methods for the preparation of these tissues for examination by transmission electron microscopy. Included within are simple methods for the isolation of cartilage-specific macromolecules, methods for immunoidentification of tissue components, bone decalcification protocols, and fixative recipes designed to stabilize cells and proteoglycan within the cartilage matrix. A discussion of cartilage prepared by high-pressure freezing (HPF)/freeze substitution (FS) is also presented.
Keene
Douglas R
DR
Shriners Hospitals for Children, Micro-Imaging Center, Portland, Oregon 97239, USA.
Tufa
Sara F
SF
D000818:Animals; D001842:Bone and Bones / Q000737:chemistry / Q000648:ultrastructure*; D002356:Cartilage / Q000737:chemistry / Q000648:ultrastructure*; D004396:Coloring Agents / Q000737:chemistry; D015925:Cryopreservation / Q000379:methods; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D046529:Microscopy, Electron, Transmission / Q000295:instrumentation / Q000379:methods*; D011509:Proteoglycans / Q000032:analysis; D016707:Tissue Fixation / Q000379:methods
D004396:Coloring Agents; D011509:Proteoglycans