title: Electrochromatographic performance of conventional and polar-embedded C16 silica monolithic stationary phases.
abstract: A novel monolithic silica column that has a polar-embedded amide-secondary amine group linking with C16 functionality for RP-CEC is described. The amide-secondary aminealkyloxysilane was synthesized by the reaction of 3-(2-aminoethylamino) propyltrimethoxysilane with hexadecanoyl chloride. Then, the silylant agent was bonded to the silica monolith matrix to produce hexadecanamide-secondary amine bonded silica (HDAIS) monolithic column. The electrochromatographic performance of HDAIS monolithic column for the separation of neutral, basic and polar solutes was studied, which was compared to that using the hexadecyl bonded silica monolithic column. The HDAIS monolithic column displayed reduced hydrophobic retention characteristics in the separation of five hydrophobic n-alkylbenzenes and four polar phenols when compared to the hexadecyl bonded silica monolithic column. A very much reduced silanol activity of HDAIS monolithic column was observed in the separation of test basic mixture including four aromatic amines, atenolol and metoprolol with 10 mM borate buffer (pH 7.5) containing 30% v/v ACN as the mobile phase. The comparison results indicate good performance for both polar and basic mixtures on HDAIS monolithic column in RP-CEC, and also show promising results for further applications.
authors.affiliation: Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education), College of Chemistry and Chemical Engineering, Guangxi Normal University, Guilin, PR China. fangguiye@163.com
authors.forename: Fanggui
authors.initials: F
authors.lastname: Ye
authors.forename: Baojun
authors.initials: B
authors.lastname: Huang
authors.forename: Shun
authors.initials: S
authors.lastname: Wang
authors.forename: Shulin
authors.initials: S
authors.lastname: Zhao
mesh_terms: D000577:Amides / Q000737:chemistry; D002021:Buffers; D053801:Capillary Electrochromatography / Q000295:instrumentation* / Q000379:methods; D006863:Hydrogen-Ion Concentration; D008855:Microscopy, Electron, Scanning; D015394:Molecular Structure; D012821:Silanes / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D000577:Amides; D002021:Buffers; D012821:Silanes; D012822:Silicon Dioxide