title: Interference of circulating endogenous antibodies on the Dimension® DGNA digoxin immunoassay: elimination with a heterophilic blocking reagent.
abstract: Only two cases have been previously described about circulating endogenous antibodies interference on the immunochemical determination of digoxin. In an elderly patient with history of social cat handling was observed a moderate positive interference (about 0.8 ng/mL) on the Dimension® DGNA digoxin immunoassay (capture rabbit antibody), which was eliminated treating the serum samples with a heterophilic blocking reagent. The Architect® immunoassay was not affected.
authors.affiliation: Unidad Monitorización Fármacos, Laboratorio Central, Hospital Clínico Universitario, Santiago de Compostela, Spain.
authors.forename: Esperanza F
authors.initials: EF
authors.lastname: Hermida-Cadahía
authors.forename: M Mar
authors.initials: MM
authors.lastname: Calvo
authors.forename: J Carlos
authors.initials: JC
authors.lastname: Tutor
mesh_terms: D000368:Aged; D000818:Animals; D000910:Antibodies, Heterophile / Q000097:blood* / Q000276:immunology; D002415:Cats; D004077:Digoxin / Q000097:blood* / Q000276:immunology; D004285:Dogs; D006801:Humans; D007118:Immunoassay / Q000379:methods* / Q000592:standards*; D008297:Male; D011817:Rabbits
chemical_list: D000910:Antibodies, Heterophile; D004077:Digoxin