A matrix solid-phase dispersion method for the extraction of seven pesticides from mango and papaya.
A simple and effective extraction method based on matrix solid-phase dispersion was developed to determine trichlorfon, pyrimethanil, methyl parathion, tetraconazole, thiabendazole, imazalil, and tebuconazole in papaya and mango using gas chromatography-mass spectrometry with selected ion monitoring. Different parameters of the method were evaluated, such as type of solid-phase (silica-gel, neutral alumina, and Florisil), the amount of solid-phase, and eluent [dichloromethane, ethyl acetate-dichloromethane (4:1, 1:4, 1:1, 2:3, v/v)]. The best results were obtained using 2.0 g of mango or papaya, 3.0 g of silica as dispersant sorbent, and ethyl acetate-dichloromethane (1:1, v/v) as eluting solvent. The method was validated using mango and papaya samples fortified with pesticides at different concentration levels (0.05, 0.10, and 1.0 mg/kg). Average recoveries (4 replicates) ranged from 80% to 146%, with relative standard deviations between 1.0% and 28%. Detection and quantification limits for mango and papaya ranged from 0.01 to 0.03 mg/kg and 0.05 to 0.10 mg/kg, respectively. The proposed method was applied to the analysis of these compounds in commercial fruit samples from a local market (Aracaju/SE, Brazil), and residues of the pesticides were not detected on the samples.
Navickiene
Sandro
S
Departamento de Química, Universidade Federal de Sergipe, Av. Marechal Rondon s/n°, Jardim Rosa Elze, 49100-000 São Cristóvão/SE, Brazil. sandnavi@ufs.br
Aquino
Adriano
A
Bezerra
Débora Santos Silva
DS
D029441:Carica / Q000737:chemistry*; D002722:Chlorobenzenes / Q000302:isolation & purification; D005504:Food Analysis / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry; D007093:Imidazoles / Q000302:isolation & purification; D031022:Mangifera / Q000737:chemistry*; D008743:Methyl Parathion / Q000302:isolation & purification; D010573:Pesticide Residues / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D010575:Pesticides / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D011743:Pyrimidines / Q000302:isolation & purification; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction / Q000379:methods*; D013827:Thiabendazole / Q000302:isolation & purification; D014230:Triazoles / Q000302:isolation & purification; D014236:Trichlorfon / Q000302:isolation & purification; D005466:Fluorocarbons
D002722:Chlorobenzenes; D007093:Imidazoles; D008743:Methyl Parathion; D010573:Pesticide Residues; D010575:Pesticides; D011743:Pyrimidines; D013827:Thiabendazole; D014230:Triazoles; D014236:Trichlorfon; C108337:pyrimethanil; C017435:enilconazole; C524826:tetraconazole; D005466:Fluorocarbons