Activation of MKK3/6, SAPK, and ATF-2/c-jun in ACL fibroblasts grown in 3 dimension collagen gels in response to application of cyclic strain.
Signal transduction pathways involved in response to cyclic tensile strain and strain deprivation in anterior cruciate ligament (ACL) fibroblasts grown in 3D collagen gels were investigated. Application of cyclic tensile strain resulted in significant activation (phosphorylation) of MKK3/6, SAPK and their downstream target transcription factors, ATF-2 and c-jun, while strain deprivation resulted in a decrease in these kinases and transcription factors. These data suggest that ACL fibroblasts cultured in 3D collagen gels respond to the mechanical environment and provide a useful system for determination of the molecular mechanisms involved in the regulation of proliferation and matrix turnover by mechanical load.
Wright
Vonda
V
Tissue Engineering, Regeneration, and Repair Program, Research Division, Hospital for Special Surgery, 535, E 70th Street, New York, New York 10021, USA.
Attia
Erik
E
Bohnert
Krista
K
Brown
Haydee
H
Bhargava
Madhu
M
Hannafin
Jo A
JA
D051698:Activating Transcription Factor 2 / Q000378:metabolism*; D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D002455:Cell Division / Q000502:physiology; D002478:Cells, Cultured; D003094:Collagen; D005109:Extracellular Matrix / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000201:enzymology*; D005782:Gels; D048031:JNK Mitogen-Activated Protein Kinases / Q000378:metabolism; D008022:Ligaments / Q000166:cytology; D048371:MAP Kinase Kinase 3 / Q000378:metabolism*; D048669:MAP Kinase Kinase 6 / Q000378:metabolism*; D020935:MAP Kinase Signaling System / Q000502:physiology*; D048055:Mitogen-Activated Protein Kinase 8 / Q000378:metabolism*; D011817:Rabbits; D013314:Stress, Mechanical; D016474:Weight-Bearing / Q000502:physiology
D051698:Activating Transcription Factor 2; D005782:Gels; D003094:Collagen; D048031:JNK Mitogen-Activated Protein Kinases; D048055:Mitogen-Activated Protein Kinase 8; D048371:MAP Kinase Kinase 3; D048669:MAP Kinase Kinase 6