[Biotransformation of artemisinic acid by cell suspension cultures of Cephalotaxus fortunei and Artemisia annua].
OBJECTIVE
To investigate the biotransformation of artemisinic acid by cell suspension cultures of Cephalotaxus fortunei and Artemisia annua.


METHODS
Artemisinic acid was added into to the media of the suspension cells of Cephalotaxus fortunei and Artemisia annua in their logarithmic growth phase. The biotransfromed product was detected with HPLC and isolated by silica gel column, Sephadex LH20 and ODS chromatography methods. The chemical structure of biotransformed product was elucidated on the basis of physical-chemical properties and spectroscopic data. Otherwise, the influence of co-cultured time on conversion ratio was investigated with HPLC.


RESULTS
One biotransformed product, 3-alpha-hydroxyartemisinic acid, was obtained after two days of artemisinic acid administration to the suspension cells of Cephalotaxus fortunei and Artemisia annua. The optimal co-cultured time in suspension cells of Cephalotaxus fortunei was 2 days with the highest biotransformation rate of 8.42%, and in the case of Artemisia annua, it was 3 days and 3.95% respectively.


CONCLUSIONS
It was the first time for the biotransformation of artemisinic acid to 3-alpha-hydroxyartemisinic acid by using cell suspension cultures of Cephalotaxus fortunei and Artemisia annua.
Hu
Yan-shan
YS
College of Pharmacy, Jinan University, Guangzhou 510632, China.
Zhu
Jian-hua
JH
Jiang
Bo
B
Yu
Rong-min
RM
D031172:Artemisia annua / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D037621:Artemisinins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D001711:Biotransformation; D002478:Cells, Cultured; D032483:Cephalotaxus / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media; D015394:Molecular Structure
C556266:3-alpha-hydroxyartemisinic acid; D037621:Artemisinins; D003470:Culture Media; C047721:artemisic acid