Site-specific biomolecule labeling with gold clusters.
Site-specific labeling of biomolecules in vitro with gold clusters can enhance the information content of electron cryomicroscopy experiments. This chapter provides a practical overview of well-established techniques for forming biomolecule/gold cluster conjugates. Three bioconjugation chemistries are covered: linker-mediated bioconjugation, direct gold-biomolecule bonding, and coordination-mediated bonding of nickel(II) nitrilotriacetic acid (NTA)-derivatized gold clusters to polyhistidine (His)-tagged proteins.
Ackerson
Christopher J
CJ
Colorado State University, Department of Chemistry, Fort Collins, Colorado, USA.
Powell
Richard D
RD
Hainfeld
James F
JF
D020285:Cryoelectron Microscopy / Q000379:methods*; D006046:Gold / Q000737:chemistry*; D006639:Histidine / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry*; D009571:Nitrilotriacetic Acid / Q000737:chemistry; D011506:Proteins / Q000737:chemistry / Q000648:ultrastructure
D011506:Proteins; C033223:polyhistidine; D006639:Histidine; D006046:Gold; D009571:Nitrilotriacetic Acid