title: S-novel SNP of alpha-lactalbumin gene in chinese dairy goats.
abstract: The alpha-lactalbumin (alpha-LA) plays a key role in lactose synthesis in mammary glands of domestic animals. Mutations in the alpha-LA gene are associated with the milk traits in dairy cattle. In our study, a novel SNP: NO_X06366:g.875 C > T was detected in 708 dairy goat individuals--268 of the Xinong Saanen breed and 440 of Guanzhong breed, which revealed a synonymous mutation in the exon 1 of alpha-LA gene. The Polymerase Chain Reaction-Single Strand Conformation Polymorphism (PCR-SSCP) and sequencing techniques showed that there were three genotypes: CC, CT and TT. Moreover, the chi2-test showed that the genotype frequencies of the two breeds were in good agreement with the Hardy-Weinberg equilibrium (P > 0.05). The relationship of the polymorphism of dairy goat alpha-LA gene with the milk trait and the body size trait was revealed. Individuals with the CC genotype were significantly smaller at chest circumference than those with CT (P < 0.05) in both breeds. But the milk trait and other body size traits of the two dairy goat breeds had no significant association with genotypes studied.
authors.affiliation: Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A & F University, Yangling, Shaanxi 712100, China.
authors.forename: R N
authors.initials: RN
authors.lastname: Ma
authors.forename: C J
authors.initials: CJ
authors.lastname: Deng
authors.forename: X M
authors.initials: XM
authors.lastname: Zhang
authors.forename: X P
authors.initials: XP
authors.lastname: Yue
authors.forename: X Y
authors.initials: XY
authors.lastname: Lan
authors.forename: H
authors.initials: H
authors.lastname: Chen
authors.forename: C Z
authors.initials: CZ
authors.lastname: Lei
mesh_terms: D000818:Animals; D049628:Body Size / Q000235:genetics; D001947:Breeding; D002681:China; D005260:Female; D005838:Genotype; D006041:Goats / Q000235:genetics*; D007768:Lactalbumin / Q000235:genetics*; D008297:Male; D020641:Polymorphism, Single Nucleotide; D018807:Polymorphism, Single-Stranded Conformational; D040641:Quantitative Trait Loci / Q000235:genetics*
chemical_list: D007768:Lactalbumin