title: Recombinant domain V of β2-glycoprotein I inhibits the formation of a 7-ketocholesteryl-9-carboxynonanoate and β2-glycoprotein I complex.
abstract: Our prior study has been reported the formation of the oxidized low-density lipoprotein (oxLDL)/β(2)-glycoproteinI (β(2)-GPI)/autoantibody complex facilitated the antiphospholipid syndrome (APS) process. The domain V of β(2)-GPI binds to the negatively charged molecules, e.g. 7-ketochoresteryl-9-caboxynonanoate (oxLig-1) derived from the oxLDL and mediates the interaction between oxLDL and β(2)-GPI. In the present study, the oxLig-1/β(2)-GPI/anti-β(2)-GPI Ab (WB-CAL-1) model was established. The recombinant domain V of β(2)-GPI (rβ(2)-GPI DV) expressed in Escherichia coli competitively inhibits the interaction between β(2)-GPI and oxLig-1 in the enzyme-linked immunoassay. Moreover, the rβ(2)-GPI DV significantly inhibits the formation of the oxLig-1/β(2)-GPI/autoantibody complex in an APS patient. The present work suggests a novel possibility that rβ(2)-GPI DV could be used to inhibit the formation of oxLDL/β(2)-GPI/autoantibody complex, and give us a hint for the development of new therapeutic strategies to prevent the APS process.
authors.affiliation: College of Bioengineering, Key Laboratory of Bio-organic Chemistry, Dalian University, 10-Xuefu Avenue, Dalian Economical and Technological Development Zone, Liaoning 116622, China.
authors.forename: Yingbiao
authors.initials: Y
authors.lastname: Zhang
authors.forename: Wenzhe
authors.initials: W
authors.lastname: Li
authors.forename: Yan
authors.initials: Y
authors.lastname: Chi
authors.forename: Renjun
authors.initials: R
authors.lastname: Wang
authors.forename: Dan
authors.initials: D
authors.lastname: Wang
authors.forename: Fan
authors.initials: F
authors.lastname: Zhang
authors.forename: Zhe
authors.initials: Z
authors.lastname: Liu
authors.forename: Eiji
authors.initials: E
authors.lastname: Matsuura
authors.forename: Qingping
authors.initials: Q
authors.lastname: Liu
mesh_terms: D016736:Antiphospholipid Syndrome / Q000097:blood / Q000276:immunology; D001323:Autoantibodies / Q000737:chemistry; D002308:Cardiolipins / Q000737:chemistry; D002788:Cholesterol Esters / Q000737:chemistry*; D006801:Humans; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry*; D053482:beta 2-Glycoprotein I / Q000737:chemistry* / Q000276:immunology
chemical_list: C510905:7-ketocholesteryl-9-carboxynonanoate; D001323:Autoantibodies; D002308:Cardiolipins; D002788:Cholesterol Esters; D011994:Recombinant Proteins; D053482:beta 2-Glycoprotein I