title: Secretion of cytokines by villous cytotrophoblast and extravillous trophoblast in the first trimester of human pregnancy.
abstract: Cytokines are proposed to play roles in regulation of trophoblast invasion, spiral artery remodeling and immunoregulation during early pregnancy. Secretion of 12 cytokines (interleukin (IL)-1β, IL-2, IL-4, IL-5, IL-8, IL-10, IL-12p70, IL-13, IFNγ, GM-CSF, MCP-1 and RANTES) by first trimester extravillous trophoblast and villous cytotrophoblast cells was examined using multiplex cytokine array technology. Seven (IL-1β, IL-8, IL-12p70, IL-13, GM-CSF, MCP-1 and RANTES) of the 12 cytokines examined were detectable in the samples studied (n=10 each group). Villous cytotrophoblast production of IL-1β and IL-8 increased with gestational age. Extravillous trophoblast production of IL-8, IL-13 and RANTES increased with gestational age. At 12-14 weeks gestation extravillous trophoblast cells secreted higher levels of IL-8, IL-13 and RANTES than villous cytotrophoblast cells.
authors.affiliation: Reproduction and Vascular Biology Group, Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, UK.
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Naruse
authors.forename: Barbara A
authors.initials: BA
authors.lastname: Innes
authors.forename: Judith N
authors.initials: JN
authors.lastname: Bulmer
authors.forename: Stephen C
authors.initials: SC
authors.lastname: Robson
authors.forename: Roger F
authors.initials: RF
authors.lastname: Searle
authors.forename: Gendie E
authors.initials: GE
authors.lastname: Lash
mesh_terms: D000328:Adult; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism*; D005260:Female; D006801:Humans; D011247:Pregnancy; D011261:Pregnancy Trimester, First / Q000378:metabolism*; D014327:Trophoblasts / Q000166:cytology / Q000378:metabolism*
chemical_list: D016207:Cytokines