Cytokine gene polymorphisms in irritable bowel syndrome in Indian population--a pilot case control study.
OBJECTIVE
This study aimed at evaluating the single nucleotide polymorphisms (SNPs) in five cytokine genes regulating inflammation at altogether 8 different loci and compared their frequencies in patients with Irritable bowel syndrome (IBS) versus healthy age and sex matched controls.


METHODS
Peripheral blood was collected for DNA cytokine analysis from 23 patients with lBS and 20 healthy controls. The cytokine SNPs studied include TNF-alpha (-308G/A), TGF-alpha1 (codon10T/C, codon25G/C), IL-6 promoter (-1082A/G; -819T/C; -592A/C), IL-6 promoter (-174G/C), and IFN-alpha (+874T/A).


RESULTS
There was a significant difference between a SNP in IL-b (-592A/C) among cases and controls. There was also a trend to significance as regards to IL-6 promoter (-174G/C). Frequencies of other SNPs were not significantly different between the two groups.


CONCLUSIONS
This pilot study shows that there are polymorphism differences in cytokine genes between patients with lBS and healthy controls from India.
Santhosh
S
S
Department of Gastrointestinal, Christian Medical College, Vellore 632 004, Tamil Nadu. sunsanthosh@gmail.com
Dutta
A K
AK
Samuel
P
P
Joseph
A J
AJ
Ashok Kumar
J
J
Kurian
George
G
D000483:Alleles; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D005260:Female; D005838:Genotype; D006801:Humans; D007194:India; D016898:Interferon-alpha / Q000235:genetics; D016753:Interleukin-10 / Q000235:genetics*; D015850:Interleukin-6 / Q000235:genetics*; D043183:Irritable Bowel Syndrome / Q000453:epidemiology / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D010865:Pilot Projects; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D018709:Statistics, Nonparametric; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
D016898:Interferon-alpha; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10