Identification of novel cyclic nucleotide phosphodiesterase gene cDNAs in the brain of guinea pig.
OBJECTIVE
Cyclic nucleotide phosphodiesterase (PDE) is a critical component of the nitric oxide (NO) signaling pathway and plays critical roles in cognition and learning, Parkinson's disease, attention deficit hyperactivity disorder, psychosis and depression. The PDEs in the brain of guinea pig have not yet been reported. The present study aimed to detect the unknown Pde cDNAs in the brain of guinea pig.


METHODS
Reverse transcription polymerase chain reaction (RT-PCR) and sequence comparison analysis were performed to detect the expression of Pde cDNAs and to assess the identity rates of cDNA and amino acid sequences between guinea pig and human or mouse, respectvely. The RT-PCR primers were located on the conserved region of human PDE and mouse Pde cDNAs.


RESULTS
Eleven novel Pde cDNAs were detected in the brain of guinea pig (Cavia porcellus), including CpPde1a, CpPde1b, CpPde2a, CpPde4a, CpPde4d, CpPde5a, CpPde6c, CpPde7b, CpPde8a, CpPde9a, and CpPde10a. The identity rates of the Pde cDNA sequences between guinea pig and human ranged from 83.8% to 94.3%, and those of the amino acid sequences ranged from 91.9% to 100%. The identity rates of Pde cDNA sequences between guinea pig and mouse ranged from 84.6% to 92.1%, and those of amino acid sequences ranged from 91.2% to 99.2%. The average identity rate of the 11 Pde cDNA sequences between guinea pig and human was significantly higher (P < 0.01) than that between guinea pig and mouse. The putative partial amino acid sequences of guinea pig contained at least one of the conserved domains of human and mouse PDE proteins.


CONCLUSIONS
These results indicate that the brain-expressed Pde genes are identified in guinea pig, which lays the foundation for further investigating the physiological roles of PDE proteins in the brain.
Long
Yue-Sheng
YS
Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and Ministry of Education of China, Institute of Neuroscience, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou 510260, China. longys1972@yahoo.com.cn
Huang
Min-Qi
MQ
Liao
Wei-Ping
WP
D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000145:classification / Q000235:genetics*; D020816:Amino Acid Motifs / Q000235:genetics; D000818:Animals; D001921:Brain / Q000201:enzymology*; D001923:Brain Chemistry / Q000235:genetics*; D018076:DNA, Complementary / Q000032:analysis*; D019143:Evolution, Molecular; D006168:Guinea Pigs; D006801:Humans; D007527:Isoenzymes / Q000145:classification / Q000235:genetics; D051379:Mice; D010802:Phylogeny; D017434:Protein Structure, Tertiary / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D012689:Sequence Homology, Nucleic Acid
D018076:DNA, Complementary; D007527:Isoenzymes; D015105:3',5'-Cyclic-AMP Phosphodiesterases