Expression of CD66 in non-Hodgkin lymphomas and multiple myeloma.
OBJECTIVE
⁹⁰Y-labeled anti-CD66b monoclonal antibody clone BW 250/183 was developed for treatment of tumors. The aim of the study was the analysis of CD66 expression in lymphoproliferative malignancies to expand the potential of anti-CD66-based therapy.


METHODS
Bone marrow samples from 260 patients were examined for the expression of CD66 on tumor cells in 104 B-chronic lymphocytic leukemias (B-CLL), 28 mantle cell lymphomas (MCL), 22 follicular lymphomas (FCL), 15 marginal zone lymphomas (MZL), 12 lymphoplasmacytic lymphomas (LPL), 13 diffuse large B cell lymphomas (DLBCL), 4 T-non-Hodgkin lymphomas (T-NHL), 3 B-NHL not otherwise specified (B-NHL NOS), 3 B acute lymphoblastic leukemias (B-ALL), and in 56 multiple myelomas (MM) by flow cytometry.


RESULTS
Positive (≥ 20%) expression of CD66abce clone Kat4c was detected in 76% of B-CLL and 76.8% of MM. The highest number of CD66abce-positive samples was in MCL and LPL (96.4% of 28 and 91.7% of 12 patients, respectively). Expression of CD66b clone BW 250/183 was examined in 114 of 260 samples. Positive expression was detected in 23.3% of B-CLL (6/35), 17.1% of MM (7/30), and 21.4% of MCL (3/14) samples.


CONCLUSIONS
The expression of CD66b compared to CD66abce was lower in all measured samples. Use of radiolabeled anti-CD66b antibody for the treatment of lymphoproliferative diseases appears to have limited preclinical substantiation.
Josef
Karban
K
First Medical Department - Clinical Department of Haematooncology, First Faculty of Medicine and General Teaching Hospital, Charles University, Prague, Czech Republic. jos.kar@tiscali.cz
Heidi
Mocikova
M
Robert
Pytlik
P
Pavel
Klener
K
Marek
Trneny
T
D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000627:therapeutic use; D015703:Antigens, CD / Q000096:biosynthesis*; D001853:Bone Marrow / Q000378:metabolism* / Q000473:pathology; D015815:Cell Adhesion Molecules / Q000096:biosynthesis*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008228:Lymphoma, Non-Hodgkin / Q000378:metabolism* / Q000473:pathology / Q000532:radiotherapy; D008297:Male; D009101:Multiple Myeloma / Q000378:metabolism* / Q000473:pathology / Q000532:radiotherapy; D016499:Radioimmunotherapy / Q000379:methods
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; C069847:CD66 antigens; D015815:Cell Adhesion Molecules