title: [Studies on the chemical constituents of Pachysandra terminalis and their antioxidant activity].
abstract: OBJECTIVE
  To study the chemical constituents from Pachysandra terminalis and their antioxidant activity.
  
  
  METHODS
  Chemical constituents were isolated by repeated column chromatography (silica gel, Toyopearl HW-40C and preparative HPLC). The structures of isolated compounds were elucidated on the basis of spectral data analysis. DPPH method was used to evaluate the free radical scavenging activity of the isolated compounds.
  
  
  RESULTS
  Six compounds were isolated and their structures were identified as follow: 2-Phenylethyl-beta-D-glucopyranoside (1), (+)Pinoresinol-4'-O-beta-D-glucopyranoside (2), Pinoresinol (3), cis-Syringin (4), 4-hydroxy-4-(3-oxo-l-butenyl)-3,5,5-trimethylcyclohex-2-en-l-one (5), 3alpha-hydroxy-5,6-epoxy-7-megastigmen-9-one (6). Compounds 2, 4, 5 showed obviously antioxidant activity, their DPPH free radical scavenging rates were 82.50%, 87.36%, and 84.56% on the concentration of 50 micromol/L, respectively.
  
  
  CONCLUSIONS
  Compounds 1-6 are isolated from this genus for the first time. Compounds 2, 4, and 5 showed significant antioxidant activities.
authors.affiliation: School of Pharmaceutical Sciences, Research Center of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, China. licy0609@126.com
authors.forename: Chen-yang
authors.initials: CY
authors.lastname: Li
authors.forename: Hui-yuan
authors.initials: HY
authors.lastname: Zhai
authors.forename: Sheng-an
authors.initials: SA
authors.lastname: Tang
authors.forename: Hong-quan
authors.initials: HQ
authors.lastname: Duan
mesh_terms: D000975:Antioxidants / Q000032:analysis* / Q000302:isolation & purification; D001713:Biphenyl Compounds / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D016166:Free Radical Scavengers; D005663:Furans / Q000032:analysis* / Q000302:isolation & purification; D005960:Glucosides / Q000032:analysis / Q000302:isolation & purification; D006027:Glycosides / Q000032:analysis* / Q000302:isolation & purification; D017705:Lignans / Q000032:analysis* / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D031236:Pachysandra / Q000737:chemistry*; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives* / Q000032:analysis / Q000302:isolation & purification; D010666:Phenylpropionates / Q000032:analysis / Q000302:isolation & purification; D010851:Picrates / Q000378:metabolism; D010936:Plant Extracts / Q000032:analysis / Q000302:isolation & purification; D010946:Plants, Medicinal / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D005663:Furans; D005960:Glucosides; D006027:Glycosides; D017705:Lignans; D010666:Phenylpropionates; D010851:Picrates; D010936:Plant Extracts; C530440:pinoresinol 4-O-glucopyranoside; C069905:phenethyl alcohol xylopyranosyl-(1-6)-glucopyranoside; C004931:1,1-diphenyl-2-picrylhydrazyl; C028305:syringin; D010626:Phenylethyl Alcohol; C103298:pinoresinol