Direct attachment of DNA to semiconducting surfaces for biosensor applications.
In this work we propose a novel method of immobilizing nucleic acids for field effect or high electron mobility transistor-based biosensors. The naturally occurring 5' terminal phosphate group on nucleic acids was used to coordinate with semiconductor and metal oxide surfaces. We demonstrate that DNA can be directly immobilized onto ZrO(2), AlGaN, GaN, and HfO(2) while retaining its ability to hybridize to target sequences with high specificity. By directly immobilizing the probe molecule to the sensor surface, as opposed to conventional crosslinking strategies, the number of steps in device fabrication is reduced. Furthermore, hybridization to target strands occurs closer to the sensor surface, which has the potential to increase device sensitivity by reducing the impact of the Debye screening length.
Fahrenkopf
Nicholas M
NM
College of Nanoscale Science and Engineering, University at Albany, 257 Fuller Road, Albany, NY, USA.
Shahedipour-Sandvik
Fatemeh
F
Tokranova
Natalya
N
Bergkvist
Magnus
M
Cady
Nathaniel C
NC
D017607:Aluminum Compounds / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D004277:DNA, Single-Stranded / Q000737:chemistry* / Q000378:metabolism; D005708:Gallium / Q000737:chemistry; D006195:Hafnium / Q000737:chemistry; D008856:Microscopy, Fluorescence; D036103:Nanotechnology; D009693:Nucleic Acid Hybridization / Q000379:methods*; D010087:Oxides / Q000737:chemistry; D012666:Semiconductors; D015040:Zirconium / Q000737:chemistry
D017607:Aluminum Compounds; D004277:DNA, Single-Stranded; D010087:Oxides; C513700:aluminum gallium nitride; C473348:gallium nitride; C545179:hafnium oxide; D015040:Zirconium; D005708:Gallium; C028541:zirconium oxide; D006195:Hafnium