title: Effect of acidification on elemental and isotopic compositions of sediment organic matter and macro-invertebrate muscle tissues in food web research.
abstract: Stable isotope techniques in food web studies often focus on organic carbon in food sources which are subsequently assimilated in the tissue of consumer organisms through diet. The presence of non-dietary carbonates in bulk samples can affect their δ(13)C values, altering how their results are interpreted. Acidification of samples is a common practice to eliminate any inorganic carbon present prior to analysis. We examined the effects of pre-analysis acidification on two size fractions of sediment organic matter (SOM) from marine and freshwater wetlands and pure muscle tissue of a common freshwater invertebrate (Cherax destructor). The elemental content and isotopic ratios of carbon and nitrogen were compared between paired samples of acidified and control treatments. Our results showed that acidification does not affect the elemental or isotopic values of freshwater SOM. In the marine environment acidification depleted the δ(13)C and δ(15)N values of the fine fraction of saltmarsh and δ(15)N values of mangrove fine SOM. Whilst acidification did not change the elemental content of invertebrate muscle tissue, the δ(13)C and δ(15)N values were affected. We recommend to researchers considering using acidification techniques on material prepared for stable isotope analysis that a formal assessment of the effect of acidification on their particular sample type should be undertaken. Further detailed investigation to understand the impact of acidification on elemental and isotopic values of organic matter and muscular tissues is required.
authors.affiliation: Australian Nuclear Science and Technology Organisation, Locked Bag 2001 Kirrawee DC, NSW 2232, Australia. debashish.mazumder@ansto.gov.au
authors.forename: Debashish
authors.initials: D
authors.lastname: Mazumder
authors.forename: Jordan
authors.initials: J
authors.lastname: Iles
authors.forename: Jeff
authors.initials: J
authors.lastname: Kelleway
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Kobayashi
authors.forename: Lisa
authors.initials: L
authors.lastname: Knowles
authors.forename: Neil
authors.initials: N
authors.lastname: Saintilan
authors.forename: Suzanne
authors.initials: S
authors.lastname: Hollins
mesh_terms: D000818:Animals; D002247:Carbon Isotopes / Q000737:chemistry; D002254:Carbonates / Q000737:chemistry; D033364:Decapoda; D020387:Food Chain; D056145:Freshwater Biology; D019015:Geologic Sediments / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008386:Marine Biology; D013058:Mass Spectrometry; D009132:Muscles / Q000737:chemistry*; D009587:Nitrogen Isotopes / Q000737:chemistry
chemical_list: D002247:Carbon Isotopes; D002254:Carbonates; D009587:Nitrogen Isotopes