ApoB-100, ApoE and CYP7A1 gene polymorphisms in Mexican patients with cholesterol gallstone disease.
OBJECTIVE
To determine the possible association of the ApoB-100 (XbaI), ApoE (HhaI) and CYP7A1 (BsaI) gene polymorphisms, with the development of cholesterol gallstone disease (GD) in a Mexican population.


METHODS
The polymorphisms were analyzed by polymerase chain reaction followed by restriction fragment length polymorphism, in two groups matched by ethnicity, age and sex: patients with GD (n = 101) and stone-free control subjects (n = 101).


RESULTS
Allelic frequencies in patients and controls were: 34.16% vs 41.58% (P = 0.124) for X+ of ApoB-100; 4.46% vs 5.94% (P = 0.501) for E2, 85.64% vs 78.22% (P = 0.052) for E3, 9.90% vs 15.84% (P = 0.075) for E4 of ApoE; and 25.74% vs 27.72% (P = 0.653) for C of CYP7A1. Differences in genotypic frequencies between the studied groups were not significant (P < 0.05).


CONCLUSIONS
These results demonstrated that no association exists between the studied polymorphisms and cholelithiasis in this high prevalent population.
Sánchez-Cuén
Jaime
J
División de Gastroenterología, Hospital Regional ISSSTE, Culiacán, Sinaloa 80230, México.
Aguilar-Medina
Maribel
M
Arámbula-Meraz
Eliakym
E
Romero-Navarro
José
J
Granados
Julio
J
Sicairos-Medina
Laura
L
Ramos-Payán
Rosalío
R
D000328:Adult; D000368:Aged; D053299:Apolipoprotein B-100 / Q000235:genetics*; D001057:Apolipoproteins E / Q000235:genetics*; D002784:Cholesterol / Q000378:metabolism*; D002790:Cholesterol 7-alpha-Hydroxylase / Q000235:genetics*; D005260:Female; D042882:Gallstones / Q000737:chemistry / Q000453:epidemiology / Q000235:genetics; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D008800:Mexico / Q000453:epidemiology; D008875:Middle Aged; D011110:Polymorphism, Genetic
D053299:Apolipoprotein B-100; D001057:Apolipoproteins E; D002784:Cholesterol; C553219:CYP7A1 protein, human; D002790:Cholesterol 7-alpha-Hydroxylase