Molecular characterization, structural analysis and determination of host range of a novel bacteriophage LSB-1.
BACKGROUND
Bacteriophages (phages) are widespread in the environment and play a crucial role in the evolution of their bacterial hosts and the emergence of new pathogens.


RESULTS
LSB-1, a reference coliphage strain, was classified as a member of the Podoviridae family with a cystic form (50 ± 5 nm diameter) and short tail (60 ± 5 nm long). The double stranded DNA was about 30 kilobase pairs in length. We identified its host range and determined the gp17 sequences and protein structure using shotgun analysis and bioinformatics technology.


CONCLUSIONS
Coliphage LSB-1 possesses a tailspike protein with endosialidase activity which is probably responsible for its specific enteroinvasive E.coli host range within the laboratory.
Chai
Yaming
Y
Department of Epidemiology, Faculty of Preventive Medicine, Third Military Medical University, Chongqing 400038, China.
Xiong
Hongyan
H
Ma
Xiangyu
X
Cheng
Liqing
L
Huang
Guorong
G
Zhang
Zhonglin Rao Lin
ZR
D000595:Amino Acid Sequence; D003090:Coliphages / Q000145:classification / Q000235:genetics* / Q000502:physiology* / Q000648:ultrastructure; D019295:Computational Biology; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D004926:Escherichia coli / Q000821:virology; D058507:Host Specificity; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010802:Phylogeny; D017902:Podoviridae / Q000145:classification / Q000235:genetics* / Q000502:physiology* / Q000648:ultrastructure; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000235:genetics; D014771:Virion / Q000648:ultrastructure
D004279:DNA, Viral; D014764:Viral Proteins