title: Small ncRNA expression and regulation under hypoxia in neural progenitor cells.
abstract: Small non-coding RNA (ncRNA) plays critical roles in a large number of cellular processes, including neural development, cell survival and cell determination. Our previous work showed that low oxygen promoted the survival and proliferation of neural progenitor cells (NPCs) in vitro. In this study, we examine the expression and regulation of small ncRNAs in the hypoxia-driven proliferation of NPCs. The expression profiles of ncRNAs in NPCs under hypoxia were detected using microarray analysis. Results of significance analysis of microarrays (SAM) revealed that 15 small RNAs were up-regulated at least threefold and 11 were down-regulated under hypoxic conditions. The differentially expressed small ncRNAs were confirmed by quantitative RT-PCR, and miR-210 was observed to be highly expressed in NPCs under hypoxic conditions. Further study showed that hypoxia-inducible factor (HIF)-1α had a direct impact on the putative promoter regions of miR-210. From these results, we conclude that some small ncRNAs participate in the regulation of the proliferation of NPCs under hypoxia and that miR-210 is directly regulated by HIF-1α.
authors.affiliation: Department of Brain Protection and Plasticity, Institute of Basic Medical Sciences, Beijing, People's Republic of China.
authors.forename: Zhao-hui
authors.initials: ZH
authors.lastname: Liu
authors.forename: Guang
authors.initials: G
authors.lastname: Yang
authors.forename: Tong
authors.initials: T
authors.lastname: Zhao
authors.forename: Guo-jun
authors.initials: GJ
authors.lastname: Cao
authors.forename: Lei
authors.initials: L
authors.lastname: Xiong
authors.forename: Wei
authors.initials: W
authors.lastname: Xia
authors.forename: Xin
authors.initials: X
authors.lastname: Huang
authors.forename: Li-ying
authors.initials: LY
authors.lastname: Wu
authors.forename: Kuiwu
authors.initials: K
authors.lastname: Wu
authors.forename: Ming
authors.initials: M
authors.lastname: Fan
authors.forename: Ning-sheng
authors.initials: NS
authors.lastname: Shao
authors.forename: Ling-ling
authors.initials: LL
authors.lastname: Zhu
mesh_terms: D000818:Animals; D015687:Cell Hypoxia / Q000235:genetics; D002478:Cells, Cultured; D004622:Embryo, Mammalian; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D046228:Microarray Analysis; D058953:Neural Stem Cells / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology*; D009474:Neurons / Q000378:metabolism / Q000502:physiology; D010100:Oxygen / Q000494:pharmacology; D058727:RNA, Small Untranslated / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D013234:Stem Cells / Q000378:metabolism / Q000502:physiology; D054928:Validation Studies as Topic
chemical_list: D058727:RNA, Small Untranslated; D010100:Oxygen