title: Hippocampal M1 receptor function associated with spatial learning and memory in aged female rhesus macaques.
abstract: Of the acetylcholine muscarinic receptors, the type 1 (M1) and type 2 (M2) receptors are expressed at the highest levels in the prefrontal cortex (PFC) and hippocampus, brain regions important for cognition. As equivocal findings of age-related changes of M1 and M2 in the nonhuman primate brain have been reported, we first assessed age-related changes in M1 and M2 in the PFC and hippocampus using saturation binding assays. Maximum M1 receptor binding, but not affinity of M1 receptor binding, decreased with age. In contrast, the affinity of M2 receptor binding, but not maximum M2 receptor binding, increased with age. To determine if in the elderly cognitive performance is associated with M1 or M2 function, we assessed muscarinic function in elderly female rhesus macaques in vivo using a scopolamine challenge pharmacological magnetic resonance imaging and in vitro using saturation binding assays. Based on their performance in a spatial maze, the animals were classified as good spatial performers (GSP) or poor spatial performers (PSP). In the hippocampus, but not PFC, the GSP group showed a greater change in T(2)*-weighted signal intensity after scopolamine challenge than the PSP group. The maximum M1 receptor binding and receptor binding affinity was greater in the GSP than the PSP group, but no group difference was found in M2 receptor binding. Parameters of circadian activity positively correlated with the difference in T(2)*-weighted signal intensity before and after the challenge, the maximum M1 receptor binding, and the M1 receptor binding affinity. Thus, while in rhesus macaques, there are age-related decreases in M1 and M2 receptor binding, in aged females, hippocampal M1, but not M2, receptor function is associated with spatial learning and memory and circadian activity.
authors.affiliation: Division of Neuroscience, Oregon National Primate Research Center, Beaverton, OR 97006, USA.
authors.forename: Gwendolen E
authors.initials: GE
authors.lastname: Haley
authors.forename: Chris
authors.initials: C
authors.lastname: Kroenke
authors.forename: Daniel
authors.initials: D
authors.lastname: Schwartz
authors.forename: Steven G
authors.initials: SG
authors.lastname: Kohama
authors.forename: Henryk F
authors.initials: HF
authors.lastname: Urbanski
authors.forename: Jacob
authors.initials: J
authors.lastname: Raber
mesh_terms: D000818:Animals; D002940:Circadian Rhythm / Q000502:physiology*; D005260:Female; D006624:Hippocampus / Q000378:metabolism*; D008253:Macaca mulatta; D008279:Magnetic Resonance Imaging; D008297:Male; D018782:Maze Learning / Q000502:physiology; D008568:Memory / Q000502:physiology*; D043643:Receptor, Muscarinic M1 / Q000378:metabolism*; D043585:Receptor, Muscarinic M2 / Q000378:metabolism; D013037:Spatial Behavior / Q000502:physiology
chemical_list: D043643:Receptor, Muscarinic M1; D043585:Receptor, Muscarinic M2