Isolation and characterization of novel protein with anti-fungal and anti-inflammatory properties from Aloe vera leaf gel.
The Aloe protein of 14 kDa from the Aloe vera leaf gel was isolated by an ion exchange chromatography using DEAE-cellulose and CM-cellulose column. The purified Aloe protein exhibited a potent anti-fungal activity against Candida paraprilosis, Candida krusei and Candida albicans. In addition, the purified Aloe protein also showed an anti-inflammatory property against pure lipoxygenase and cyclooxygenase-2 with 84% and 73% inhibition, respectively, and was verified by binding with these proteins by real time method by the phenomenon of surface plasmon resonance. This Aloe protein is a novel protein possessing antifungal and anti-inflammatory properties and thus sets a platform to be used as a medicinal plant product.
Das
Swagata
S
Department of Biophysics, All India Institute of Medical Sciences, Ansari Nagar, New Delhi 110029, India.
Mishra
Biswajit
B
Gill
Kamaldeep
K
Ashraf
Md Saquib
MS
Singh
Abhay Kumar
AK
Sinha
Mou
M
Sharma
Sujata
S
Xess
Immaculata
I
Dalal
Krishna
K
Singh
Tej Pal
TP
Dey
Sharmistha
S
D000504:Aloe / Q000737:chemistry*; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D000935:Antifungal Agents / Q000494:pharmacology*; D002175:Candida / Q000187:drug effects; D051546:Cyclooxygenase 2 / Q000378:metabolism; D003636:DEAE-Cellulose; D057075:Enzyme Assays; D005782:Gels; D006384:Hemagglutination / Q000187:drug effects; D006801:Humans; D008084:Lipoxygenase / Q000378:metabolism; D008826:Microbial Sensitivity Tests; D010447:Peptide Hydrolases / Q000378:metabolism; D018515:Plant Leaves / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D011480:Protease Inhibitors / Q000494:pharmacology; D020539:Sequence Analysis, Protein; D017386:Sequence Homology, Amino Acid; D013053:Spectrophotometry
D000893:Anti-Inflammatory Agents; D000935:Antifungal Agents; D005782:Gels; D010940:Plant Proteins; D011480:Protease Inhibitors; D003636:DEAE-Cellulose; D008084:Lipoxygenase; D051546:Cyclooxygenase 2; D010447:Peptide Hydrolases