title: Identification of a plant gene encoding glutamate/aspartate-prephenate aminotransferase: the last homeless enzyme of aromatic amino acids biosynthesis.
abstract: In all organisms synthesising phenylalanine and/or tyrosine via arogenate, a prephenate aminotransferase is required for the transamination of prephenate into arogenate. The identity of the gene encoding this enzyme in the organisms where this activity occurs is still unknown. Glutamate/aspartate-prephenate aminotransferase (PAT) is thus the last homeless enzyme in the aromatic amino acids pathway. We report on the purification, mass spectrometry identification and biochemical characterization of Arabidopsis thaliana prephenate aminotransferase. Our data revealed that this activity is housed by the prokaryotic-type plastidic aspartate aminotransferase (At2g22250). This represents the first identification of a gene encoding PAT.
authors.affiliation: Université Grenoble 1, Grenoble, France.
authors.forename: Matthieu
authors.initials: M
authors.lastname: Graindorge
authors.forename: Cécile
authors.initials: C
authors.lastname: Giustini
authors.forename: Anne Claire
authors.initials: AC
authors.lastname: Jacomin
authors.forename: Alexandra
authors.initials: A
authors.lastname: Kraut
authors.forename: Gilles
authors.initials: G
authors.lastname: Curien
authors.forename: Michel
authors.initials: M
authors.lastname: Matringe
mesh_terms: D024322:Amino Acids, Aromatic / Q000378:metabolism*; D017360:Arabidopsis / Q000166:cytology / Q000201:enzymology / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D001219:Aspartate Aminotransferases / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D018698:Glutamic Acid / Q000378:metabolism; D007700:Kinetics; D013058:Mass Spectrometry; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism; D000637:Transaminases / Q000235:genetics / Q000378:metabolism*
chemical_list: D024322:Amino Acids, Aromatic; D029681:Arabidopsis Proteins; D011994:Recombinant Proteins; D018698:Glutamic Acid; D000637:Transaminases; C044954:prephenate aminotransferase; D001219:Aspartate Aminotransferases