Novel portable hypothermic pulsatile perfusion preservation technology: Improved viability and function of rodent and canine kidneys.
BACKGROUND
A reliable, portable, cost effective device for perfusion preservation of donor organs remains elusive. A portable, organ perfusion device design for hypothermic, machine perfusion (HMP) that successfully supported rodent kidneys for 24 hours was evaluated in canine kidneys.


METHODS
Freshly recovered rodent and canine kidneys were subjected to 24 hours of HMP or static storage (SS). Organ perfusion and cell membrane integrity were examined in HMP and SS rodent kidneys. Canine kidney function was evaluated in an isolated organ preparation. Oxygen consumption (OC), renal vascular resistance (RVR), and glomerular filtration rates (GFR) were compared.


RESULTS
Perfusion pressure during HMP averaged 16 mmHg with oxygen delivery roughly 4 fold greater than the canine kidney's metabolic requirements. Following 24 hours of preservation, RVR was significantly elevated while OC and GFR were significantly lower in the SS organs compared to the HMP stored or freshly recovered kidneys.


CONCLUSIONS
This organ preservation technology appears to provide an excellent preservation environment for kidneys such that post-transplant delayed graft function is minimized. Additionally, compared to current machine perfusion systems, the preservation system described in this work is significantly reduced in size, weight, and complexity, such that total portability may be possible.
Tolstykh
Gleb P
GP
Department of Anesthesiology, University of Texas Health Science Center at San Antonio, 78229, USA.
Gelineau
Jerry F
JF
Maier
Lisa M
LM
Bunegin
Leonid
L
D000818:Animals; D050377:Cold Ischemia; D004285:Dogs; D004867:Equipment Design; D005919:Glomerular Filtration Rate / Q000502:physiology; D007668:Kidney / Q000502:physiology* / Q000503:physiopathology; D009926:Organ Preservation / Q000295:instrumentation / Q000379:methods*; D010101:Oxygen Consumption; D010477:Perfusion / Q000295:instrumentation*; D011673:Pulsatile Flow / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D014655:Vascular Resistance / Q000502:physiology