In vitro and in vivo efficacy of Acorus calamus extract against Rhipicephalus (Boophilus) microplus.
To develop a environment friendly control measure against cattle tick, Rhipicephalus (Boophilus) microplus, medicinally important plants were identified and extracts were prepared. Twelve 95% ethanolic, thirteen 50% hydroethanolic and nine hot water extracts were prepared and tested against laboratory reared homogenous colony of R. (B.) microplus. Amongst the 34 extracts, 26 extracts showed no mortality within 72 h of application while 12.0 ± 4.9% to 35.0 ± 9.6% mortality of treated ticks was recorded in other extracts. Of the effective extracts, the extract prepared from rhizome of Acorus calamus proved highly efficacious and 100% final mortality within 14 DPT was recorded. The LC85 value of the extract was determined as 11.26. In vivo experiments confirmed the efficacy of the extract up to 42%, and repeat application was required after 7 DPT. The extract was found safe and no reaction was observed when animals were treated with 50% of the concentration, which was five times of the concentration used for in vivo studies. The possibility of using the extract for the control of cattle tick is discussed.
Ghosh
Srikanta
S
Entomology Laboratory, Parasitology Division, Indian Veterinary Research Institute, Izatnagar, 243122, Bareilly, UP, India. sghoshp@yahoo.co.in
Sharma
Anil Kumar
AK
Kumar
Sachin
S
Tiwari
Shashi Shankar
SS
Rastogi
Subha
S
Srivastava
Sharad
S
Singh
Mahima
M
Kumar
Rinesh
R
Paul
Souvik
S
Ray
D D
DD
Rawat
Ajay Kumar Singh
AK
D056810:Acaricides / Q000494:pharmacology* / Q000633:toxicity; D029023:Acorus / Q000737:chemistry*; D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000188:drug therapy* / Q000469:parasitology; D002851:Chromatography, High Pressure Liquid; D005260:Female; D007814:Larva / Q000187:drug effects; D008136:Longevity / Q000187:drug effects; D008297:Male; D010058:Oviposition / Q000187:drug effects; D021261:Parasitic Sensitivity Tests; D010936:Plant Extracts / Q000494:pharmacology* / Q000633:toxicity; D011817:Rabbits; D048494:Rhipicephalus / Q000187:drug effects*; D012867:Skin / Q000187:drug effects / Q000473:pathology; D013984:Tick Infestations / Q000188:drug therapy / Q000469:parasitology / Q000662:veterinary*; D018675:Toxicity Tests
D056810:Acaricides; D010936:Plant Extracts