Azirine ligation: fast and selective protein conjugation via photoinduced azirine-alkene cycloaddition.
We report a new bioorthogonal ligation reaction between p-nitrodiphenylazirine and dimethyl fumarate. This photoinduced azirine-alkene cycloaddition provides a rapid (~2 min) and highly selective route to protein conjugation at neutral pH and room temperature in biological medium.
Lim
Reyna K V
RK
Department of Chemistry, State University of New York at Buffalo, Buffalo, New York 14260, USA.
Lin
Qing
Q
D000475:Alkenes / Q000737:chemistry*; D001389:Azirines / Q000737:chemistry*; D003500:Cyclization; D010777:Photochemistry; D011506:Proteins / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization
D000475:Alkenes; D001389:Azirines; D011506:Proteins