[Effects of 650 nm laser and moxibustion pretreatment on enteric nervous system and medullary visceral zone in rats with visceral traction pain].
OBJECTIVE
To explore effects of 650 nm laser and moxibustion pretreatment on visceral traction pain (VTP) and its mechanism.


METHODS
Forty male SD rats were randomly devided into a sham operation group (group A), a VTP group (group B), a 650 nm laser pretreatment group (group C), a moxibustion pretreatment group (group D). Rats in group A and group B were not treated except sham operation or VTP model. In group C and D, the VTP models were produced immediate after 650 nm laser irradiation or moxibustion at "Zusanli" (ST 36), respectively. The changes of pain score and systolic pressure were investigated and the activity of AChE, the content of SP and leu-enkephaline (LEK), and the positive index of c-Fos protein and glial fibrillary acidic protein (GFAP) were detected by biochemistry, radio-immunity method and immunohistochemistry, respectively.


RESULTS
Compared with group A, the pain score, systolic pressure, the activity of AChE, the content of SP, and the positive index of c-Fos protein and GFAP of group B increased significantly (all P < 0.05); compared with group B, the pain score, AChE activity, the content of SP and the positive index of c-Fos protein and GFAP of both group C and group D decreased significantly (all P < 0.05); compared with group B, the content of LEK increased and systolic pressure decreased significantly in group C (both P < 0.05).


CONCLUSIONS
Both 650 nm laser and moxibustion pretreatment can inhibit VTP and the mechanism may be related to reducing the activity of AChE and the content of SP, and increasing the activity of LEK and decreasing the expression of c-Fos protein and GFAP.
Yang
Hua-Yuan
HY
Acupuncture and Massage College, Shanghai University of TCM, Shanghai 201203, China. yhyabcd@sina.com
Guo
Ting-Ting
TT
Ma
Yi-Nan
YN
Liu
Tang-Yi
TY
Gao
Ming
M
D000818:Animals; D003131:Combined Modality Therapy; D017615:Enteric Nervous System / Q000503:physiopathology*; D006801:Humans; D007418:Intestinal Pseudo-Obstruction / Q000503:physiopathology / Q000628:therapy*; D053685:Laser Therapy; D008297:Male; D009071:Moxibustion; D010146:Pain / Q000503:physiopathology; D059408:Pain Management; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley