Volatile anesthetics modulate gene expression in breast and brain tumor cells.
Gene expression is increasingly used for diagnostic, prognostic, and therapeutic purposes in clinical practice. We tested the hypothesis that volatile anesthetics (VA) affect gene expression of tumor cells. Cells from the neuronal cell line SH-SY5Y and from the breast cell line MCF-7 were exposed ex vivo to enflurane, isoflurane, desflurane, halothane, sevoflurane, or nitrous oxide. Microarray gene expression profiles were studied. We observed significant differences in gene expression levels of cell cultures and response in time when exposed to different VA. Some genes used for predictive genetic fingerprints for breast cancer were affected by VA. Our findings suggest that VA modulate gene expression in breast and brain tumor cell cultures in a unique and time-dependent manner.
Huitink
Johannes M
JM
Department of Anesthesiology, VU University Medical Center Amsterdam, De Boelelaan 1117, 1081 HV Amsterdam, The Netherlands. j.huitink@vumc.nl
Heimerikxs
Mike
M
Nieuwland
Marja
M
Loer
Stephan A
SA
Brugman
Wim
W
Velds
Arno
A
Sie
Daoud
D
Kerkhoven
Ron M
RM
D018685:Anesthetics, Inhalation / Q000494:pharmacology*; D001932:Brain Neoplasms / Q000235:genetics*; D001943:Breast Neoplasms / Q000235:genetics*; D045744:Cell Line, Tumor; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D013997:Time Factors
D018685:Anesthetics, Inhalation