title: DNA variation in the gene ELTD1 is associated with tick burden in cattle.
abstract: Ticks and tick-born diseases are major constraints on cattle production in tropical and subtropical regions in the world. Previously, we identified single nucleotide polymorphisms (SNPs) associated with tick resistance on bovine chromosome 3 at approximately 70 Mb. In this study, we genotyped a dairy (n = 1133) and a beef (n = 774) sample to confirm the association of the intronic SNP rs29019303 and its gene (ELTD1) with tick burden. We genotyped 18 additional SNPs in a region of 181 kb and found that rs29019303 was significantly (P < 0.05) associated with tick burden in both samples with the same favourable allele. A second SNP in this same genomic region was also significantly associated with tick burden in each sample. The associations using haplotypes were stronger than for single markers, including a haplotype of nine tag SNPs that was highly significantly (P = 0.0008) associated with tick counts in the dairy animals. This haplotype and two others were significant after Bonferroni correction for multiple testing. The estimated size of the effects was close to 0.9% of the residual variance in both samples tested.
authors.affiliation: Cooperative Research Centre for Beef Genetic Technologies, CSIRO Livestock Industries, Queensland Bioscience Precinct, St Lucia, QLD 4067, Australia.
authors.forename: L R
authors.initials: LR
authors.lastname: Porto Neto
authors.forename: R J
authors.initials: RJ
authors.lastname: Bunch
authors.forename: B E
authors.initials: BE
authors.lastname: Harrison
authors.forename: W
authors.initials: W
authors.lastname: Barendse
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000235:genetics* / Q000469:parasitology; D020022:Genetic Predisposition to Disease; D020641:Polymorphism, Single Nucleotide; D013984:Tick Infestations / Q000235:genetics / Q000469:parasitology / Q000662:veterinary*; D013987:Ticks