title: Claudin-6, 7, or 9 overexpression in the human gastric adenocarcinoma cell line AGS increases its invasiveness, migration, and proliferation rate.
abstract: Altered claudin expression is related to metastatic potential, poor prognosis, or tumor recurrence. We analyzed if the overexpression of claudin-6, claudin-7, or claudin-9 in AGS cells altered cell motility, invasiveness, or proliferation rate. Claudin-7, claudin-9, and claudin-6 enhanced their invasive potential by 3.4-fold, 1.6-fold, and 2.0-fold, respectively. Claudin-6 and claudin-9 enhanced cell migration, while the proliferation rate of claudin-6-, claudin-7-, and claudin-9-transfected cells increased by 12.7%, 9.0%, and 13.3%, respectively. Claudin-7 and claudin-9 overexpression increased claudin-1 and zonula occludens-1 levels. In summary, individual increased expression of claudin-6, claudin-7, or claudin-9 is sufficient to enhance tumorigenic properties of a gastric adenocarcinoma cell line.
authors.affiliation: Depto. Bioquímica, Facultad de Medicina, UNAM, México.
authors.forename: Veronica Elizabeth
authors.initials: VE
authors.lastname: Zavala-Zendejas
authors.forename: Ana Cristina
authors.initials: AC
authors.lastname: Torres-Martinez
authors.forename: Berenice
authors.initials: B
authors.lastname: Salas-Morales
authors.forename: Teresa Imelda
authors.initials: TI
authors.lastname: Fortoul
authors.forename: Luis Felipe
authors.initials: LF
authors.lastname: Montaño
authors.forename: Erika Patricia
authors.initials: EP
authors.lastname: Rendon-Huerta
mesh_terms: D000199:Actins / Q000378:metabolism; D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D062445:Claudin-1; D057167:Claudins; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D010750:Phosphoproteins / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D022502:Stress Fibers / Q000378:metabolism; D013997:Time Factors; D014162:Transfection; D015854:Up-Regulation; D062826:Zonula Occludens-1 Protein
chemical_list: D000199:Actins; C568108:CLDN1 protein, human; C491875:CLDN2 protein, human; C473255:CLDN7 protein, human; C524757:CLDN9 protein, human; D062445:Claudin-1; D057167:Claudins; D008565:Membrane Proteins; D010750:Phosphoproteins; D012333:RNA, Messenger; C569042:TJP1 protein, human; D062826:Zonula Occludens-1 Protein; C122101:claudin 6