title: Expression of genes involved in rat liver angiogenesis after ischaemia and reperfusion: effects of ischaemic pre- and post-conditioning.
abstract: BACKGROUND
  During surgery, ischaemic pre- (IPC) and post-conditioning (IPO) protects the liver against ischaemia/reperfusion injuries (I/R-injuries). The impact of ischaemic conditioning on liver regeneration has been less well studied. Angiogenesis is an important part of liver regeneration after hepatectomy. The aim of the present study was to investigate the effect of ischaemia/reperfusion and ischaemic conditioning on the expression of genes with angiogenic potential in a model of rat liver ischaemia.
  
  
  METHODS
  A model of total liver ischaemia (30 min) and reperfusion (30 min) was employed using Wistar rats. Rats were randomized into five groups: (C) control (IRI) ischaemic, IPC, IPO and IPC + IPO. Liver enzymes were sampled at the end of reperfusion. Liver biopsies were analysed using cDNA microarrays.
  
  
  RESULTS
  Alanine aminotransferase (ALT) increased significantly in all the ischaemic groups compared with controls (P= 0.000). Searching databases 99 genes involved in rat liver angiogenesis were identified. Compared with group (C) the number of genes significantly up-regulated was as follows: IRI (n= 5), IPC (n= 24), IPO (n= 33) and IPC + IPO (n= 18). No genes were down-regulated in the four groups compared with controls.
  
  
  CONCLUSIONS
  Ischaemic conditioning, as demonstrated in the present study, seems to be potent activators of angiogenic genes. This might be favourable to the regenerating liver.
authors.affiliation: Department of Surgical Gastroenterology L, Aarhus University Hospital, Aarhus C, Denmark. auknudsen@gmail.com
authors.forename: Anders R
authors.initials: AR
authors.lastname: Knudsen
authors.forename: Anne-Sofie
authors.initials: AS
authors.lastname: Kannerup
authors.forename: Rune
authors.initials: R
authors.lastname: Dich
authors.forename: Mogens
authors.initials: M
authors.lastname: Kruhøffer
authors.forename: Peter
authors.initials: P
authors.lastname: Funch-Jensen
authors.forename: Henning
authors.initials: H
authors.lastname: Grønbaek
authors.forename: Frank V
authors.initials: FV
authors.lastname: Mortensen
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001706:Biopsy; D016000:Cluster Analysis; D030541:Databases, Genetic; D004195:Disease Models, Animal; D020869:Gene Expression Profiling / Q000379:methods; D005786:Gene Expression Regulation; D057775:Ischemic Postconditioning; D019194:Ischemic Preconditioning; D008099:Liver / Q000098:blood supply* / Q000201:enzymology; D008297:Male; D018919:Neovascularization, Physiologic / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000235:genetics / Q000503:physiopathology / Q000517:prevention & control*; D013997:Time Factors
chemical_list: D000410:Alanine Transaminase