title: Nucleotide polymorphisms and the 5'-UTR transcriptional analysis of the bovine growth hormone secretagogue receptor 1a (GHSR1a) gene.
abstract: Growth hormone secretagogue receptor 1a (GHSR1a) mediates the different actions of its endogenous ligand, ghrelin. Ghrelin-GHSR is involved in many important functions that include growth hormone secretion and food intake. We evaluated the haplotype variety and characterized the microsatellite ((TG)(n) , 5'-UTR) and nucleotide polymorphisms of the bovine GHSR1a gene. The nucleotide sequencing of this gene (∼6 kb) revealed 47 single nucleotide polymorphisms (SNPs), four indels and the microsatellite ((GTTT)(n) , Intron 1). The 19 haplotypes were constructed from all nucleotide viability patterns and were divided into three major groups. Four SNPs (L24V, nt456(G>A), D191N and nt667(C>T)) and DelR242 in Exon 1 and a haplotype block of approximately 2.2 kb (nt667(C>T) ∼ nt2884 (A>G)) were found in Bos taurus breeds. Breed differences in allele frequencies of the two microsatellites, nt-7(C>A), L24V, and DelR242 loci were found (P < 0.005). A DelR242 was found in the Japanese Shorthorn (frequency: ∼ 0.44), Japanese Brown, five European cattle breeds, the Philippine native cattle, but none detected in the Japanese Black or the Mishima island cattle. Additionally, 5'-rapid amplification of cDNA ends and RT-PCR analyses revealed that there were two different kinds of transcripts: spliced, without a microsatellite within 5'-UTR (GHSR1a); and non-spliced, with the microsatellite (GHSR1b).
authors.affiliation: National Institute of Livestock and Grassland Science National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, Japan. mkomatsu@affrc.go.jp
authors.forename: Masanori
authors.initials: M
authors.lastname: Komatsu
authors.forename: Yuki
authors.initials: Y
authors.lastname: Fujimori
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Sato
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Okamura
authors.forename: Seiki
authors.initials: S
authors.lastname: Sasaki
authors.forename: Tomohito
authors.initials: T
authors.lastname: Itoh
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Morita
authors.forename: Ryoichi
authors.initials: R
authors.lastname: Nakamura
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Oe
authors.forename: Masako
authors.initials: M
authors.lastname: Furuta
authors.forename: Junpei
authors.initials: J
authors.lastname: Yasuda
authors.forename: Takatoshi
authors.initials: T
authors.lastname: Kojima
authors.forename: Toshio
authors.initials: T
authors.lastname: Watanabe
authors.forename: Takeshi
authors.initials: T
authors.lastname: Hayashi
authors.forename: Aduli E O
authors.initials: AE
authors.lastname: Malau-Aduli
authors.forename: Hideaki
authors.initials: H
authors.lastname: Takahashi
mesh_terms: D020121:5' Untranslated Regions; D000818:Animals; D002417:Cattle / Q000235:genetics*; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide; D054440:Receptors, Ghrelin / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D020121:5' Untranslated Regions; D054440:Receptors, Ghrelin