High-salt diet advances molecular circadian rhythms in mouse peripheral tissues.
Dietary compounds influence the expression of various genes and play a major role in changing physiological and metabolic states. However, little is known about the role of food ingredients in the regulation of circadian gene expression. Here, we show that feeding mice with a high-salt (HS) diet ad libitum for over 2weeks advanced the phase of clock gene expression by about 3h in the liver, kidney, and lung, but did not change circadian feeding, drinking, and locomotor rhythms. Focused DNA microarray analysis showed that the expression phase of many genes related to metabolism in the liver was also advanced. Immediately before phase advancement in peripheral tissues, the mRNA expression of sodium-glucose cotransporter 1 (Sglt1) and glucose transporter 2 (Glut2), that are responsible for glucose absorption, was increased in the jejunum. Furthermore, blood glucose uptake increased more rapidly after consuming the HS diet than the control diet. Moreover, phloridzin, a specific inhibitor of SGLT1, prevented the increased glucose transporter expression in the jejunum and phase advancement in the livers of mice on the HS diet. These results suggest that increased glucose absorption induced by dietary HS alters the food entrainment of peripheral molecular circadian rhythms.
Oike
Hideaki
H
Food Function Division, National Food Research Institute (NFRI), National Agriculture and Food Research Organization (NARO), Tsukuba, Ibaraki 305-8642, Japan. oike@affrc.go.jp
Nagai
Kanji
K
Fukushima
Tatsunobu
T
Ishida
Norio
N
Kobori
Masuko
M
D000818:Animals; D002940:Circadian Rhythm / Q000187:drug effects* / Q000235:genetics; D004032:Diet; D015870:Gene Expression / Q000187:drug effects*; D005947:Glucose / Q000378:metabolism; D007408:Intestinal Absorption / Q000187:drug effects; D007583:Jejunum / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010695:Phlorhizin / Q000494:pharmacology; D012965:Sodium Chloride / Q000008:administration & dosage*; D051269:Sodium-Glucose Transporter 1 / Q000037:antagonists & inhibitors
C495675:Slc5a1 protein, mouse; D051269:Sodium-Glucose Transporter 1; D012965:Sodium Chloride; D010695:Phlorhizin; D005947:Glucose