Experimental studies of OH° radical/pressure dependence of arginase activity using a molecular chromatography approach.
Arginase is an enzyme which plays a role in pathophysiology such as hypertension. Here we demonstrated for the first time the direct implication of pressure and OH° radical formation on the arginase activity via a novel analytical procedure. Pressure increased arginase activity in the range 12-52bars. Activation by OH° radical showed a hyperbolic response. The OH° radicals produced were significantly inhibited by sulfasalazine (SAZ) and the inhibition of OH° radicals parallels the inhibition of arginase activity.
André
Claire
C
Laboratoire de Chimie Analytique et Physique EA4267, Faculté de Médecine Pharmacie, Université de Franche-Comté, Place Saint Jacques, 25030 Besançon Cedex, France.
Ibrahim
Firas
F
Gharbi
Tijani
T
Herlem
Guillaume
G
Guillaume
Yves Claude
YC
D000818:Animals; D001119:Arginase / Q000737:chemistry* / Q000378:metabolism; D019149:Bioreactors; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D017665:Hydroxyl Radical / Q000737:chemistry* / Q000494:pharmacology*; D007700:Kinetics; D011312:Pressure; D012460:Sulfasalazine / Q000494:pharmacology
D004800:Enzymes, Immobilized; D017665:Hydroxyl Radical; D012460:Sulfasalazine; D001119:Arginase