Defects and DNA replication.
We introduce a rate-equation formalism to study DNA replication kinetics in the presence of defects resulting from DNA damage and find a crossover between two regimes: a normal regime, where the influence of defects is local, and an initiation-limited regime. In the latter, defects have a global impact on replication, whose progress is set by the rate at which origins of replication are activated, or initiated. Normal, healthy cells have defect densities in the normal regime. Our model can explain an observed correlation between interorigin separation and rate of DNA replication.
Gauthier
Michel G
MG
Department of Physics, Simon Fraser University, Burnaby, British Columbia, Canada, V5A 1S6.
Herrick
John
J
Bechhoefer
John
J
D000818:Animals; D004247:DNA / Q000096:biosynthesis* / Q000235:genetics*; D004249:DNA Damage; D004260:DNA Repair; D004261:DNA Replication; D006801:Humans; D007700:Kinetics; D008954:Models, Biological
D004247:DNA