title: Disruption of telomere-telomere interactions associated with DNA damage in human spermatozoa.
abstract: Telomeres play a fundamental role in the organization of the sperm nucleus resulting in the looped chromosome configuration and non-random positioning of chromosomes. Telomeres localize in the nuclear periphery and interact dynamically by forming dimers and tetramers. The purpose of this study was to evaluate the relationship of telomere interactions to DNA damage, a factor known to adversely influence male fertility. Telomeres were localized by fluorescence in situ hybridization (FISH) using human chromosome pan-telomeric probe in ten samples with low and ten samples with high sperm DNA damage. The samples with a low DNA fragmentation index (DFI) had a mean number of telomere signals of 21.7±1.9 compared to a mean of 26.5±3.4 signals in the samples with a high DFI (p<.005). The percentage of cells with a typical telomere distribution of ≤23 telomere-telomere dimers was observed in 70.8%±15.6 samples with a low DFI compared to 44.2%±22.4 in samples with a high DFI (p<.05). These results suggest that sperm DNA damage is associated with disruption of the normal telomere-telomere interactions leading to possible loss of the looped chromosome configuration. Improperly packed and organized sperm chromatin might have a high probability of disrupting the extremely structured sequence of sperm chromosome deposition, activation, and processing by the oocyte at the time of fertilization. These results might provide additional information on the nature of sperm DNA damage and the role of such damage on fertilization and development of the zygote.
authors.affiliation: Andrology Laboratory, Department of Pathology and Laboratory Medicine, Mount Sinai Hospital, Toronto, Ontario, Canada. smoskovtsev@mtsinai.on.ca
authors.forename: Sergey I
authors.initials: SI
authors.lastname: Moskovtsev
authors.forename: Jennifer
authors.initials: J
authors.lastname: Willis
authors.forename: John
authors.initials: J
authors.lastname: White
authors.forename: J Brendan M
authors.initials: JB
authors.lastname: Mullen
mesh_terms: D002843:Chromatin / Q000648:ultrastructure; D053938:DNA Fragmentation; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007248:Infertility, Male / Q000235:genetics*; D008297:Male; D013094:Spermatozoa / Q000502:physiology*; D016615:Telomere / Q000502:physiology* / Q000648:ultrastructure
chemical_list: D002843:Chromatin