Investigating possible changes in protein structure during dendrimer-protein binding.
Building on our previous results that revealed a sized based mechanism for dendrimer/protein binding, the mechanism of complexation is further probed using CD spectroscopy; the results demonstrate that dendrimer/protein binding is not accompanied by changes in the protein's structure and that binding takes place on the interfacial area/active site entrance.
Chiba
F
F
Chemistry Department, University of Sheffield, Sheffield, UKS3 7HF.
Mann
G
G
Twyman
L J
LJ
D002918:Chymotrypsin / Q000737:chemistry* / Q000378:metabolism*; D002942:Circular Dichroism; D045304:Cytochromes c / Q000737:chemistry* / Q000378:metabolism*; D050091:Dendrimers / Q000737:chemistry* / Q000378:metabolism*; D011485:Protein Binding
D050091:Dendrimers; D045304:Cytochromes c; D002918:Chymotrypsin