title: Genotoxic and cytotoxic effects induced by aluminum in the lymphocytes of the common carp (Cyprinus carpio).
abstract: Few studies have been made in regard to the effect of aluminum on the molecular and cellular structure and function of aquatic organisms; therefore, in the present report we determined the genotoxic and cytotoxic effects induced by the metal on the lymphocytes of carp (Cyprinus carpio). Three groups of fish were exposed to 0.05, 120, and 239 mg/L of aluminum (Al), respectively, by using Al₂ (SO₄)₃·7H₂O, and another group was included as control. The cells obtained were studied with the comet assay, flow cytometry, and the TUNEL method. With the first method we found a concentration and time dependent, significant increase in the amount of DNA damage induced by Al, and a higher damage when we evaluated the level of oxidized DNA. By applying flow cytometry we established that the metal induced a DNA content increase and ploidy modifications as well as apoptosis and disturbances of the cell cycle progression. With the last method we determined a significant increase in the amount of apoptotic cells, mainly in the 72-96 h period. Our results established that Al caused deleterious DNA and cellular effects in the tested organism, and they suggested the pertinence of evaluating toxicity induced by the metal in organisms living in contaminated water bodies.
authors.affiliation: Laboratorio de Toxicología Acuática, Escuela Nacional de Ciencias Biológicas, IPN, México.
authors.forename: Sandra
authors.initials: S
authors.lastname: García-Medina
authors.forename: Celene
authors.initials: C
authors.lastname: Razo-Estrada
authors.forename: Marcela
authors.initials: M
authors.lastname: Galar-Martinez
authors.forename: Edith
authors.initials: E
authors.lastname: Cortéz-Barberena
authors.forename: Leobardo Manuel
authors.initials: LM
authors.lastname: Gómez-Oliván
authors.forename: Isela
authors.initials: I
authors.lastname: Alvarez-González
authors.forename: Eduardo
authors.initials: E
authors.lastname: Madrigal-Bujaidar
mesh_terms: D000535:Aluminum / Q000633:toxicity*; D000818:Animals; D002347:Carps; D003603:Cytotoxins / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D008214:Lymphocytes / Q000187:drug effects*; D009153:Mutagens / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000633:toxicity
chemical_list: D003603:Cytotoxins; D009153:Mutagens; D014874:Water Pollutants, Chemical; D000535:Aluminum