Electromagnetic induction detector for capillary electrophoresis and its application in pharmaceutical analysis.
A new electromagnetic induction detector for capillary electrophoresis and its application are described. The detector is consisted of an inductor, a resistor, a high-frequency signal generator and a high-frequency millivoltmeter. The conditions affecting the response of the detector, including dimension of the magnetic ring, position of the capillary, number of coil turns, frequency, excitation voltage and value of the resistor were examined and optimized. The feasibility of the proposed detector was evaluated by detection of inorganic ions and separation of amino aids. Its quantification applicability was investigated by determination of aspirin and paracetamol in pharmaceutical preparation (Akafen powder). The primary factors affecting separation efficiency, which include variety of buffer, buffer concentration, injection time and injection height and separation voltage, were researched. Experimental results demonstrated that this new detector showed a well-defined correlation between sample concentrations and responses (r=0.997-0.999), with detection limits of 30 μmol L(-1) for aspirin and 10 μmol L(-1) for paracetamol, as well as good reproducibility and stability. Compared with currently available detection techniques, this new detector has several advantages, such as simple construction, no complicated elements, ease of assembly and operation, and potential for universal applications. It can be an alternative to the traditional methods in the quality control of the pharmaceutical preparations.
Yang
Xiu-Juan
XJ
School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, Guangdong 510006, PR China.
Chen
Zuan-Guang
ZG
Liu
Cui
C
Li
Ou-Lian
OL
D000082:Acetaminophen / Q000032:analysis*; D001241:Aspirin / Q000032:analysis*; D004574:Electromagnetic Fields; D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000379:methods*; D007202:Indicators and Reagents; D057230:Limit of Detection; D004364:Pharmaceutical Preparations / Q000032:analysis* / Q000592:standards; D011786:Quality Control; D012996:Solutions
D007202:Indicators and Reagents; D004364:Pharmaceutical Preparations; D012996:Solutions; D000082:Acetaminophen; D001241:Aspirin