title: Genomic characterization of Campylobacter jejuni strain M1.
abstract: Campylobacter jejuni strain M1 (laboratory designation 99/308) is a rarely documented case of direct transmission of C. jejuni from chicken to a person, resulting in enteritis. We have sequenced the genome of C. jejuni strain M1, and compared this to 12 other C. jejuni sequenced genomes currently publicly available. Compared to these, M1 is closest to strain 81116. Based on the 13 genome sequences, we have identified the C. jejuni pan-genome, as well as the core genome, the auxiliary genes, and genes unique between strains M1 and 81116. The pan-genome contains 2,427 gene families, whilst the core genome comprised 1,295 gene families, or about two-thirds of the gene content of the average of the sequenced C. jejuni genomes. Various comparison and visualization tools were applied to the 13 C. jejuni genome sequences, including a species pan- and core genome plot, a BLAST Matrix and a BLAST Atlas. Trees based on 16S rRNA sequences and on the total gene families in each genome are presented. The findings are discussed in the background of the proven virulence potential of M1.
authors.affiliation: Department of Systems Biology, The Technical University of Denmark, Lyngby, Denmark. carsten@cbs.dtu.dk
authors.forename: Carsten
authors.initials: C
authors.lastname: Friis
authors.forename: Trudy M
authors.initials: TM
authors.lastname: Wassenaar
authors.forename: Muhammad A
authors.initials: MA
authors.lastname: Javed
authors.forename: Lars
authors.initials: L
authors.lastname: Snipen
authors.forename: Karin
authors.initials: K
authors.lastname: Lagesen
authors.forename: Peter F
authors.initials: PF
authors.lastname: Hallin
authors.forename: Diane G
authors.initials: DG
authors.lastname: Newell
authors.forename: Monique
authors.initials: M
authors.lastname: Toszeghy
authors.forename: Anne
authors.initials: A
authors.lastname: Ridley
authors.forename: Georgina
authors.initials: G
authors.lastname: Manning
authors.forename: David W
authors.initials: DW
authors.lastname: Ussery
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D002169:Campylobacter Infections / Q000382:microbiology* / Q000635:transmission / Q000662:veterinary*; D016123:Campylobacter jejuni / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D002645:Chickens; D002874:Chromosome Mapping; D016680:Genome, Bacterial; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D011201:Poultry Diseases / Q000382:microbiology*
chemical_list: D001426:Bacterial Proteins