A simple strategy for the construction of combinatorial cyclic peptoid libraries.
Here we describe a simple method that allows for rapid and easy sequence determination of cyclic peptoids. The key idea in this strategy is a post-screening "ring-opening" reaction to convert cyclic peptoids selected from a high-throughput screen into linear peptoids, which can be sequenced by tandem mass spectrometry. Thus, there is no need for encoding.
Lee
Ji Hoon
JH
Department of Biochemistry and Molecular Biology, Indiana University Simon Cancer Center, Indiana University School of Medicine, Indianapolis, Indiana 46202, USA.
Meyer
Amanda M
AM
Lim
Hyun-Suk
HS
D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D020650:Combinatorial Chemistry Techniques / Q000379:methods*; D007700:Kinetics; D008856:Microscopy, Fluorescence; D019151:Peptide Library; D010456:Peptides, Cyclic / Q000138:chemical synthesis* / Q000737:chemistry; D034444:Peptoids / Q000138:chemical synthesis* / Q000737:chemistry; D053719:Tandem Mass Spectrometry
D019151:Peptide Library; D010456:Peptides, Cyclic; D034444:Peptoids