Sevoflurane postconditioning ameliorates oxygen-glucose deprivation-reperfusion injury in the rat hippocampus.
BACKGROUND
Sevoflurane is well known to exert a neuroprotective effect through anesthetic preconditioning. However, its effects on postconditioning, a neuroprotective phenomenon following an insult, have not been well studied.


OBJECTIVE
In this study, we examined the ability of sevoflurane to induce postconditioning in rat hippocampal slices, in vitro.


RESULTS
2%, 4%, and 6% sevoflurane reduced neurophysiologic and morphologic neuronal injury following oxygen-glucose deprivation (OGD) and reperfusion. The quantity of damaged neurons was significantly reduced on immunofluorescence staining; excitatory amino acids (Asp, Glu) increased and inhibitory amino acids (GABA) decreased significantly. The effect was concentration-dependent.


CONCLUSIONS
Postconditioning with sevoflurane reduces neuronal damage after OGD-reperfusion injury in the CA1 area of rat hippocampus, in vitro.
Peng
Sheng
S
Department of Anesthesiology, Affiliated No. 4 Hospital of Suzhou University, Wuxi, PR China.
Kalikiri
Pramood
P
Mychaskiw
George
G
Zhang
Dengxin
D
Zhang
Yan
Y
Liu
Gong-Jian
GJ
Wang
Guang-Lei
GL
Shen
Zhi-yun
ZY
D000596:Amino Acids / Q000134:cerebrospinal fluid / Q000378:metabolism; D018685:Anesthetics, Inhalation / Q000494:pharmacology*; D000818:Animals; D004396:Coloring Agents; D055724:Electrophysiological Phenomena; D005071:Evoked Potentials / Q000502:physiology; D005455:Fluorescent Antibody Technique; D005947:Glucose / Q000172:deficiency*; D006624:Hippocampus / Q000187:drug effects / Q000473:pathology*; D002534:Hypoxia, Brain / Q000188:drug therapy* / Q000473:pathology; D008297:Male; D008738:Methyl Ethers / Q000494:pharmacology*; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D009474:Neurons / Q000473:pathology; D018696:Neuroprotective Agents; D018377:Neurotransmitter Agents / Q000134:cerebrospinal fluid / Q000378:metabolism; D011419:Propidium; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000473:pathology; D000077149:Sevoflurane
D000596:Amino Acids; D018685:Anesthetics, Inhalation; D004396:Coloring Agents; D008738:Methyl Ethers; D018696:Neuroprotective Agents; D018377:Neurotransmitter Agents; D011419:Propidium; D000077149:Sevoflurane; D005947:Glucose