Origin and molecular organization of supernumerary chromosomes of Prochilodus lineatus (characiformes, prochilodontidae) obtained by DNA probes.
In Prochilodus lineatus B-chromosomes are visualized as reduced size extra elements identified as microchromosomes and are variable in morphology and number. We describe the specific total probe (B-chromosome probe) in P. lineatus obtained by chromosome microdissection and a whole genomic probe (genomic probe) from an individual without B-chromosome. The specific B-chromosome was scraped and processed to obtain DNA with amplification by DOP-PCR, and so did the genomic probe DNA. Fluorescence in situ hybridization using the B-chromosome probe labeled with dUTP-Tetramethyl-rhodamine and the genomic probe labeled with digoxigenin-FITC permitted to establish that in this species supernumerary chromosomes with varying number and morphology had different structure of chromatin when compared to that of the regular chromosomes or A complement, since only these extra elements were labeled in the metaphases. The present findings suggest that modifications in the chromatin structure of B-chromosomes to differentiate them from the A chromosomes could occur along their dispersion in the individuals of the population.
Voltolin
Tatiana Aparecida
TA
Laboratório de Genética de Peixes, Departamento Ciências Biológicas, Faculdade de Ciências, Universidade Estadual Paulista (UNESP), Campus de Bauru, Av. Engenheiro Luiz Edmundo C. Coube, s/n, 17033-360 Bauru, SP, Brazil.
Laudicina
Alejandro
A
Senhorini
José Augusto
JA
Bortolozzi
Jehud
J
Oliveira
Cláudio
C
Foresti
Fausto
F
Porto-Foresti
Fábio
F
D000818:Animals; D002843:Chromatin; D002875:Chromosomes; D020732:Cytogenetic Analysis; D015342:DNA Probes / Q000235:genetics*; D019143:Evolution, Molecular; D005260:Female; D005399:Fishes / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D008297:Male
D002843:Chromatin; D015342:DNA Probes