title: The expression analysis of Notch-1 and Jagged-2 during the development of the hindgut in rat embryos with ethylenethiourea induced anorectal malformations.
abstract: BACKGROUND
  This study was designed to investigate the expression of Notch-1 and Jagged-2 in the terminal hindgut in ethylenethiourea (ETU)-exposed rat embryos with anorectal malformations (ARMs) and its potential association with the maldevelopment of the terminal hindgut in ARMs.
  
  
  METHODS
  ETU-exposed ARMs model was introduced to investigate the expression pattern of Notch-1 and Jagged-2 during the hindgut development using immunohistochemical staining, reverse transcriptase polymerase chain reaction (RT-PCR) and Western blot analysis.
  
  
  RESULTS
  Immunostaining revealed that the expression of Notch-1 and Jagged-2 showed changes in the developing terminal hindgut of ARMs. The expression of Notch-1 and Jagged-2 in the terminal hindgut of ARMs rat embryos decreased at both mRNA level and protein level (P < 0.05) compared with normal tissues.
  
  
  CONCLUSIONS
  This study demonstrated that the expression of Notch-1 and Jagged-2 in ARMs of ETU-exposed rat embryos was remarkably reduced, which implied its potential role in the pathogenesis of the terminal hindgut maldevelopment in ARMs.
authors.affiliation: Department of Pediatric Surgery, Shengjing Hospital, China Medical University, Shenyang, P.R. China.
authors.forename: Huimin
authors.initials: H
authors.lastname: Jia
authors.forename: Qingjiang
authors.initials: Q
authors.lastname: Chen
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
authors.forename: Yuzuo
authors.initials: Y
authors.lastname: Bai
authors.forename: Zhengwei
authors.initials: Z
authors.lastname: Yuan
authors.forename: Weilin
authors.initials: W
authors.lastname: Wang
mesh_terms: D000818:Animals; D000071056:Anorectal Malformations; D001006:Anus, Imperforate / Q000139:chemically induced* / Q000378:metabolism*; D005031:Ethylenethiourea / Q000009:adverse effects*; D041981:Gastrointestinal Tract / Q000196:embryology* / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D000072101:Jagged-2 Protein; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism*; D023421:Models, Animal; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D051881:Receptor, Notch1 / Q000378:metabolism*
chemical_list: C490502:Jag2 protein, rat; D000072101:Jagged-2 Protein; D008565:Membrane Proteins; C497763:Notch1 protein, rat; D012333:RNA, Messenger; D051881:Receptor, Notch1; D005031:Ethylenethiourea