title: Antineutrophil cytoplasmic antibodies against myeloperoxidase, proteinase 3, elastase, cathepsin G and lactoferrin in Japanese patients with rheumatoid arthritis.
abstract: Antineutrophil cytoplasmic antibodies (ANCAs) against myeloperoxidase (MPO), proteinase 3 (PR-3), lactoferrin (LF), cathepsin G (CG) and elastase (EL) were determined to investigate whether the presence of ANCAs is closely related to extra-articular manifestations in Japanese patients with rheumatoid arthritis (RA). Antibodies against MPO, PR-3, LF, CG and EL were determined in sera from 125 patients with RA and 83 sera from patients with other rheumatic diseases by enzyme-linked immunosorbent assay. Clinical manifestations and laboratory parameters of the patients were studied from medical records. Thirty of the 125 (24.0%) RA patients were positive for ANCAs for at least one of these 5 ANCA antigens. Among the 5 ANCAs, anti-LF antibody (anti-LF) (16.8%) was most commonly observed in patients with RA. A higher joint score (JS) and an elevated ESR were demonstrated in ANCA-positive RA patients compared to those of ANCA-negative patients (40.8 ± 43.3, 24.3 ± 26.2, p < 0.05, 44.4 ± 22.4, 28.9 ± 23.6, p < 0.05, respectively). No statistical differences in the presence of interstitial pneumonia, cutaneous vasculitis, rheumatoid nodules and mononeuropathy multiplex were observed between ANCA-positive and ANCA-negative patients. The presence of anti-LF is expected to be of pathological relevance, as the action of anti-LF towards LF results in the inhibition of the anti-inflammatory activity of LF.
authors.affiliation: Department of Internal Medicine, Juntendo Koshigaya Hospital, 560 Fukuroyama, Koshigaya, Saitama, 343-0032, Japan. kida@juntendo.ac.jp
authors.forename: Issei
authors.initials: I
authors.lastname: Kida
authors.forename: Shigeto
authors.initials: S
authors.lastname: Kobayashi
authors.forename: Ken
authors.initials: K
authors.lastname: Takeuchi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Tsuda
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hashimoto
authors.forename: Yoshinari
authors.initials: Y
authors.lastname: Takasaki
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D019268:Antibodies, Antineutrophil Cytoplasmic / Q000097:blood*; D001172:Arthritis, Rheumatoid / Q000097:blood / Q000175:diagnosis / Q000276:immunology*; D001175:Arthrography; D015415:Biomarkers / Q000032:analysis; D056649:Cathepsin G / Q000276:immunology; D004798:Enzymes / Q000276:immunology*; D005260:Female; D006801:Humans; D007596:Joints / Q000473:pathology; D007781:Lactoferrin / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D053146:Myeloblastin / Q000276:immunology; D010196:Pancreatic Elastase / Q000276:immunology; D009195:Peroxidase / Q000276:immunology; D014657:Vasculitis / Q000097:blood / Q000175:diagnosis / Q000276:immunology; D055815:Young Adult
chemical_list: D019268:Antibodies, Antineutrophil Cytoplasmic; D015415:Biomarkers; D004798:Enzymes; C490098:LTF protein, human; D009195:Peroxidase; D007781:Lactoferrin; D056649:Cathepsin G; D010196:Pancreatic Elastase; D053146:Myeloblastin