Characterization of phenolic content, in vitro biological activity, and pesticide loads of extracts from white grape skins from organic and conventional cultivars.
Grape skin extracts of Riesling Vitis vinifera L. grapes from conventionally or organically managed cultivars were compared on the basis of their phenolic content, antioxidant capacity, antimicrobial and antimutagenic properties and pesticide loads. Promising results on their biological properties suggest that those extracts would be valuable as food preservatives. The antioxidant capacity of conventional extracts was significantly higher, according to the higher content in catechin, epicatechin and procyanidin B. Pesticide loads did not affect the antimutagenic or antimicrobial properties of the extracts. Both extracts inhibited the growth of Gram-positive foodborne pathogens such as Staphylococcus aureus, Enterococcus faecalis and Enterococcus faecium to similar extents. Possibly as a result of higher amounts of quercetin and its derivatives, higher antimicrobial effects against Listeria monocytogenes and Salmonella typhimurium were observed for the organic white grape skin extracts. Conventional or organic extracts did not show remarkable antimutagenic effects when tested against the mutagen IQ by means of the Ames test. Due to the presence of fungicides, the conidial germination of Penicillium expansum, Penicillium chrysogenum and Aspergillus niger, were inhibited by 95% by conventional GSE, while negligible effects were observed with organic grape extracts. The latter, however, showed inhibitory effects against Trichoderma viridie and Aspergillus versicolor.
Corrales
Margarita
M
Max Rubner-Institute, Department of Safety and Quality of Fruit and Vegetables, Karlsruhe, Germany. margarita.corrales@ec.europa.eu
Fernandez
Avelina
A
Vizoso Pinto
Maria G
MG
Butz
Peter
P
Franz
Charles M A P
CM
Schuele
Eberhard
E
Tauscher
Bernhard
B
D000975:Antioxidants / Q000032:analysis / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D002241:Carbohydrates / Q000032:analysis; D002839:Chromans / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D005419:Flavonoids / Q000032:analysis; D058870:Food, Organic / Q000032:analysis*; D005638:Fruit / Q000737:chemistry; D005658:Fungi / Q000187:drug effects; D007202:Indicators and Reagents; D000432:Methanol; D009584:Nitrogen / Q000032:analysis; D010573:Pesticide Residues / Q000032:analysis*; D010636:Phenols / Q000032:analysis* / Q000494:pharmacology*; D010936:Plant Extracts / Q000032:analysis; D011188:Potassium / Q000032:analysis; D012997:Solvents; D021241:Spectrometry, Mass, Electrospray Ionization; D014466:Ultraviolet Rays; D027843:Vitis / Q000737:chemistry* / Q000382:microbiology
D000975:Antioxidants; D002241:Carbohydrates; D002839:Chromans; D005419:Flavonoids; D007202:Indicators and Reagents; D010573:Pesticide Residues; D010636:Phenols; D010936:Plant Extracts; D012997:Solvents; D009584:Nitrogen; D011188:Potassium; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid; D000432:Methanol