[Structural characterization of a glucan isolated from Gastrodia elata].
OBJECTIVE
To study a glucan (GB II) isolated from Gastrodia elata.


METHODS
The glucan was obtained with water extraction, alcohol precipitate, DEAE-Sepharose Fast Flow column and Sepharose CL-6B column chromatography; sugar composition analysis, IR and NMR were used to determine the structural feature.


RESULTS
The molecular weight of the glucan was 4 300 dalton estimated by HPGPC; it contained 27 glucose residues, which mainly composed of alpha-D-(1-->4)-glucose with little glucuronic acid and branch O-6 points.


CONCLUSIONS
The glucan was a new glucan for the first report from Gastrodia elata.
Hong
Qi-ming
QM
Shanghai University of Traditional Chinese Medicine, Institute of Chinese Materia Medica, Shanghai 201210, China.
Shi
Song-shan
SS
Wang
Cai
C
Wang
Shun-chun
SC
Li
Xi-lin
XL
Zhou
Xiu-jia
XJ
D008401:Gas Chromatography-Mass Spectrometry; D031668:Gastrodia / Q000737:chemistry*; D005936:Glucans / Q000737:chemistry* / Q000302:isolation & purification; D020723:Glucuronic Acid / Q000032:analysis* / Q000302:isolation & purification; D008745:Methylation; D015394:Molecular Structure; D008970:Molecular Weight; D009005:Monosaccharides / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D035281:Plant Tubers / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D013055:Spectrophotometry, Infrared
D005936:Glucans; D009005:Monosaccharides; D020723:Glucuronic Acid