title: [Screening of candidate proteins interacting with Foxp3δ2 by yeast two-hybrid system].
abstract: OBJECTIVE
  To screen Foxp3Delta2-interacting proteins by yeast two-hybria.
  
  
  METHODS
  pGBKT7-Foxp3Delta2 bait plasmid was constructed. Its toxicity and transcriptional activation in yeast cell AH109 was tested by observation of phenotypes and color.
  
  
  RESULTS
  The bait plasmid pGBKT7-Foxp3Delta2 was constructed successfully and there was no self-activation or toxicity in AH109. Forty positive clones were obtained. After sequence comparison and analysis, nine candidate proteins were finally selected for further confirmation.
  
  
  CONCLUSIONS
  Yeast two hybrid system could be used for screening Foxp3Delta2-interacting proteins. These results provide essential clues for further investigation of Foxp3Delta2 function.
authors.affiliation: Biotechnology Center, Pharmacy Department, Fourth Military Medical University, Xi'an, China.
authors.forename: Yu-jin
authors.initials: YJ
authors.lastname: Xu
authors.forename: Chang-li
authors.initials: CL
authors.lastname: Jiang
authors.forename: Cun
authors.initials: C
authors.lastname: Zhang
authors.forename: Xin
authors.initials: X
authors.lastname: Qin
authors.forename: Meng
authors.initials: M
authors.lastname: Li
authors.forename: Qiang
authors.initials: Q
authors.lastname: Hao
authors.forename: Wei-na
authors.initials: WN
authors.lastname: Li
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Ying-qi
authors.initials: YQ
authors.lastname: Zhang
mesh_terms: D051858:Forkhead Transcription Factors / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008297:Male; D011485:Protein Binding; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D020798:Two-Hybrid System Techniques
chemical_list: C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors