Effect of crowding on the conformation of interwound DNA strands from neutron scattering measurements and Monte Carlo simulations.
With a view to determining the distance between the two opposing duplexes in supercoiled DNA, we have measured small angle neutron scattering from pHSG298 plasmid (2675 base pairs) dispersed in saline solutions. Experiments were carried out under full and zero average DNA neutron scattering contrast using hydrogenated plasmid and a 1:1 mixture of hydrogenated and perdeuterated plasmid, respectively. In the condition of zero average contrast, the scattering intensity is directly proportional to the single DNA molecule scattering function (form factor), irrespective of the DNA concentration and without complications from intermolecular interference. The form factors are interpreted with Monte Carlo computer simulation. For this purpose, the many body problem of a dense DNA solution was reduced to the one of a single DNA molecule in a congested state by confinement in a cylindrical potential. It was observed that the interduplex distance decreases with increasing concentration of salt as well as plasmid. Therefore, besides ionic strength, DNA crowding is shown to be important in controlling the interwound structure and site juxtaposition of distal segments of supercoiled DNA. This first study exploiting zero average DNA contrast has been made possible by the availability of perdeuterated plasmid.
Zhu
Xiaoying
X
Department of Physics, National University of Singapore, Singapore.
Ng
Siow Yee
SY
Gupta
Amar Nath
AN
Feng
Yuan Ping
YP
Ho
Bow
B
Lapp
Alain
A
Egelhaaf
Stefan U
SU
Forsyth
V Trevor
VT
Haertlein
Michael
M
Moulin
Martine
M
Schweins
Ralf
R
van der Maarel
Johan R C
JR
D001703:Biophysics / Q000379:methods; D003198:Computer Simulation; D004247:DNA / Q000737:chemistry*; D004278:DNA, Superhelical / Q000737:chemistry*; D017666:Deuterium Oxide; D007477:Ions; D009010:Monte Carlo Method; D009502:Neutrons; D009690:Nucleic Acid Conformation; D010957:Plasmids / Q000378:metabolism; D012492:Salts / Q000737:chemistry / Q000494:pharmacology; D012542:Scattering, Radiation; D012997:Solvents / Q000737:chemistry; D014867:Water / Q000737:chemistry
D004278:DNA, Superhelical; D007477:Ions; D012492:Salts; D012997:Solvents; D014867:Water; D004247:DNA; D017666:Deuterium Oxide