Bone marrow stromal cells cultured on poly (lactide-co-glycolide)/nano-hydroxyapatite composites with chemical immobilization of Arg-Gly-Asp peptide and preliminary bone regeneration of mandibular defect thereof.
Polyethyleneimine (PEI) was used to create active groups on the poly (lactide-co-glycolide)/nano-hydroxyapatite (PLGA/NHA) surface and Arg-Gly-Asp (RGD) was grafted on the active groups and novel PLGA/NHA 2-D membranes and 3D scaffolds modified with RGD were obtained. X-ray photoelectron spectrum (XPS) results show that sulfur displays only on the modified surface. The RGD-modified PLGA/NHA materials also have much lower static water contact angle and much higher water-absorption ability, which shows that after chemical treatment, the modified materials show better hydrophilic properties. Atomic force microscope (AFM) shows that after surface modification, the surface morphology of PLGA is greatly changed. All these results indicate that RGD peptide has successfully grafted on the surface of PLGA. Rabbit bone marrow stromal cells (MSCs) were seeded in the 2D membranes and 3D scaffolds materials. The influences of the RGD on the cell attachment, growth and differentiation, and proliferation on the different materials were studied. The modified scaffolds were implanted into rabbits to observe preliminary application in regeneration of mandibular defect. The PLGA/NHA-RGD presents better results in bone regeneration in rabbit mandibular defect.
Huang
Yanxia
Y
Institute of Nano- and Biopolymeric Materials, School of Material Science and Engineering, Tongji University, Shanghai, People's Republic of China.
Ren
Jie
J
Ren
Tianbin
T
Gu
Shuying
S
Tan
Qinggang
Q
Zhang
Lihong
L
Lv
Kaige
K
Pan
Kefeng
K
Jiang
Xinquan
X
D000042:Absorption / Q000187:drug effects; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology; D001854:Bone Marrow Cells / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure; D001861:Bone Regeneration / Q000187:drug effects*; D002448:Cell Adhesion / Q000187:drug effects; D002452:Cell Count; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002478:Cells, Cultured; D017886:Durapatite / Q000494:pharmacology*; D055767:Immobilized Proteins / Q000494:pharmacology; D019344:Lactic Acid / Q000494:pharmacology*; D008334:Mandible / Q000000981:diagnostic imaging / Q000187:drug effects / Q000473:pathology*; D018625:Microscopy, Atomic Force; D049329:Nanostructures / Q000737:chemistry*; D009842:Oligopeptides / Q000494:pharmacology*; D056951:Photoelectron Spectroscopy; D011100:Polyglycolic Acid / Q000494:pharmacology*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011817:Rabbits; D011859:Radiography; D017154:Stromal Cells / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure; D013997:Time Factors; D054457:Tissue Scaffolds / Q000737:chemistry; D014867:Water
D001672:Biocompatible Materials; D055767:Immobilized Proteins; D009842:Oligopeptides; D014867:Water; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; C047981:arginyl-glycyl-aspartic acid; D017886:Durapatite; D000469:Alkaline Phosphatase