title: Evaluation of serum autoantibody levels in the diagnosis of ovarian endometrioma.
abstract: OBJECTIVE
  We analyzed autoantibodies against tumor-associated antigens (TAAs) in the serum of patients with endometrioma and healthy controls to determine whether autoantibodies can be accurate biomarkers for the diagnosis of ovarian endometrioma.
  
  
  METHODS
  Serum samples were obtained from 56 patients with endometriosis and 66 healthy women who served as normal controls. The titers of antibodies against a panel of eight TAAs were analyzed using enzyme-linked immunosorbent assay.
  
  
  RESULTS
  We found that the serum IGFII mRNA-binding protein 1 (IMP1) autoantibody and cyclin B1 autoantibody could discriminate between healthy controls and endometriosis patients (AUC-ROC 0.777; 95% confidence interval [CI] 0.694-0.860, P<0.0005, and AUC-ROC 0.614; 95%confidence interval [CI] 0.513-0.714, P=0.031, respectively). Using 0.073 and 0.007 as the cutoff values for IMP1 and Cyclin B1 autoantibody, respectively, the sensitivity and specificity of IMP1 were 85.7 and 63.6%, respectively. When cylcin B1 was combined with IMP1, the specificity increased to 72.7% and the sensitivity slightly decreased to 83.9%.
  
  
  CONCLUSIONS
  Our data suggest that IMP1 alone or combined with cyclin B1 seems to fulfill the requirements of sensitivity and specificity to become a useful clinical biomarker of endometrioma. However, further studies will be required to establish the predictive value and to support the clinical use of IMP1/cyclin B1 in the diagnosis and/or screening of endometriosis.
authors.affiliation: Department of Obstetrics and Gynecology, Taichung Veterans General Hospital, Taichung, Taiwan.
authors.forename: Yu-Chiao
authors.initials: YC
authors.lastname: Yi
authors.forename: Shih-Chi
authors.initials: SC
authors.lastname: Wang
authors.forename: Chun-Chin
authors.initials: CC
authors.lastname: Chao
authors.forename: Chia-Ling
authors.initials: CL
authors.lastname: Su
authors.forename: Yao-Ling
authors.initials: YL
authors.lastname: Lee
authors.forename: Ling-Yun
authors.initials: LY
authors.lastname: Chen
mesh_terms: D000293:Adolescent; D000328:Adult; D001323:Autoantibodies / Q000097:blood*; D014408:Biomarkers, Tumor / Q000276:immunology*; D016022:Case-Control Studies; D056744:Cyclin B1 / Q000276:immunology*; D004715:Endometriosis / Q000175:diagnosis*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008875:Middle Aged; D010051:Ovarian Neoplasms / Q000175:diagnosis*; D011379:Prognosis; D016601:RNA-Binding Proteins / Q000276:immunology*; D012680:Sensitivity and Specificity; D055815:Young Adult
chemical_list: D001323:Autoantibodies; D014408:Biomarkers, Tumor; C534699:CCNB1 protein, human; D056744:Cyclin B1; C406144:IGF2BP1 protein, human; D016601:RNA-Binding Proteins