Serine/threonine protein phosphatase assays.
Methods for assaying serine/threonine protein phosphatases are discussed. Three commonly used protocols are presented that employ either colorimetric or radiometric assays. These methods can be used to assay a variety of preparations of serine/threonine phosphatases, from crude lysates to purified proteins. Strategies for the application of a particular protocol for a particular purpose are discussed. The assays can be used in either a high-throughput mode where simple comparison of activities can be done, or in specific assays where kinetic data can be derived.
McAvoy
Thomas
T
The Rockefeller University, New York, New York, USA.
Nairn
Angus C
AC
D003124:Colorimetry / Q000379:methods; D057075:Enzyme Assays / Q000379:methods*; D010749:Phosphoprotein Phosphatases / Q000378:metabolism*; D011874:Radiometry / Q000379:methods; D012694:Serine / Q000378:metabolism; D013912:Threonine / Q000378:metabolism
D013912:Threonine; D012694:Serine; D010749:Phosphoprotein Phosphatases