Growth factor receptor-bound protein 14: a new modulator of photoreceptor-specific cyclic-nucleotide-gated channel.
Growth factor receptor-bound protein 14 (Grb14) is an adaptor protein that is involved in receptor tyrosine kinase signalling. In this study, we report that Grb14 interacts with the rod photoreceptor-specific cyclic-nucleotide-gated channel alpha subunit (CNGA1) and decreases its affinity for cyclic guanosine monophosphate. Channel modulation is controlled by direct binding of the Grb14 Ras-associating domain with the carboxy-terminal region of CNGA1. We observed that the channel remains open in Grb14(-/-) mice that are exposed to light, suggesting that Grb14 is a normal physiological modulator of CNG channel function in vivo.
Gupta
Vivek K
VK
Department of Ophthalmology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73104, USA.
Rajala
Ammaji
A
Daly
Roger J
RJ
Rajala
Raju V S
RV
D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002417:Cattle; D002462:Cell Membrane / Q000378:metabolism; D054815:Cyclic Nucleotide-Gated Cation Channels / Q000378:metabolism*; D003624:Darkness; D057809:HEK293 Cells; D006801:Humans; D007700:Kinetics; D051379:Mice; D009928:Organ Specificity; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D021122:Protein Subunits / Q000378:metabolism; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D051381:Rats; D012374:Rod Cell Outer Segment / Q000378:metabolism*
D048868:Adaptor Proteins, Signal Transducing; D054815:Cyclic Nucleotide-Gated Cation Channels; C490977:Grb14 protein, mouse; C490976:Grb14 protein, rat; D021122:Protein Subunits; D011506:Proteins; D002118:Calcium