title: Naïve and memory B cells in the rhesus macaque can be differentiated by surface expression of CD27 and have differential responses to CD40 ligation.
abstract: The rhesus macaque (RM) model has the potential to be an invaluable tool for studying B cell populations during pathogenic infections, however, to date, there has been no definitive delineation of naïve and memory B cell populations in the RM. This has precluded a rigorous analysis of the generation, persistence and resolution of a pathogen-specific memory B cell response. The present study utilized multiple analyses to demonstrate that CD27 expression on B cells is consistent with a memory phenotype. Compared to CD20+CD27- B cells, CD20+CD27+ B cells were larger in size, and preferentially accumulated at effector sites. Direct sequence analysis revealed that CD20+CD27+ B cells had an increased frequency of point mutations that were consistent with somatic hypermutation and at a functional level, CD40 ligation improved CD20+CD27- but not CD20+CD27+ B cell survival in vitro. These data provide definitive evidence that the naïve and memory B cell populations of the RM can be differentiated using surface expression of CD27.
authors.affiliation: Department of Immunology, University of Pittsburgh, Pittsburgh PA 15261, USA.
authors.forename: David
authors.initials: D
authors.lastname: Kuhrt
authors.forename: Seth
authors.initials: S
authors.lastname: Faith
authors.forename: Angela
authors.initials: A
authors.lastname: Hattemer
authors.forename: Amanda
authors.initials: A
authors.lastname: Leone
authors.forename: Donald
authors.initials: D
authors.lastname: Sodora
authors.forename: Louis
authors.initials: L
authors.lastname: Picker
authors.forename: Lisa
authors.initials: L
authors.lastname: Borghesi
authors.forename: Kelly Stefano
authors.initials: KS
authors.lastname: Cole
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology*; D001483:Base Sequence; D015415:Biomarkers / Q000097:blood; D019013:CD40 Antigens / Q000276:immunology; D023201:CD40 Ligand / Q000276:immunology*; D005434:Flow Cytometry / Q000662:veterinary; D007156:Immunologic Memory / Q000276:immunology*; D016130:Immunophenotyping / Q000662:veterinary; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000276:immunology; D008253:Macaca mulatta / Q000097:blood / Q000276:immunology*; D008969:Molecular Sequence Data; D012313:RNA / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D027041:Somatic Hypermutation, Immunoglobulin / Q000276:immunology; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7 / Q000096:biosynthesis* / Q000097:blood / Q000276:immunology
chemical_list: D015415:Biomarkers; D019013:CD40 Antigens; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7; D023201:CD40 Ligand; D012313:RNA