Validation of suitable endogenous control genes for expression studies of miRNA in prostate cancer tissues.
When performing quantitative polymerase chain reaction analysis, there is a need for correction of technical variation between experiments. This correction is most commonly performed by using endogenous control genes, which are stably expressed across samples, as reference genes for normal expression in a specific tissue. In microRNA (miRNA) studies, two types of control genes are commonly used: small nuclear RNAs and small nucleolar RNAs. In this study, six different endogenous control genes for miRNA studies were investigated in prostate tissue material from the Swedish Watchful Waiting cohort. The stability of the controls was investigated using two different software applications, NormFinder and BestKeeper. RNU24 was the most suitable endogenous control gene for miRNA studies in prostate tissue materials.
Carlsson
Jessica
J
Systems Biology Research Center-Tumor Biology, Department of Life Sciences, University of Skövde, Skövde, Sweden.
Helenius
Gisela
G
Karlsson
Mats
M
Lubovac
Zelmina
Z
Andrén
Ove
O
Olsson
Björn
B
Klinga-Levan
Karin
K
D020869:Gene Expression Profiling / Q000379:methods*; D015972:Gene Expression Regulation, Neoplastic; D052138:Genes, Neoplasm / Q000235:genetics*; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D011471:Prostatic Neoplasms / Q000235:genetics*; D012015:Reference Standards; D015203:Reproducibility of Results
D035683:MicroRNAs