title: Effects of differently polarized microwave radiation on the microscopic structure of the nuclei in human fibroblasts.
abstract: To investigate the influence of microwave radiation on the human fibroblast nuclei, the effects of three variants of electromagnetic wave polarization, linear and left-handed and right-handed elliptically polarized, were examined. Experimental conditions were: frequency (f) 36.65 GHz, power density (P) at the surface of exposed object 1, 10, 30, and 100 µW/cm(2), exposure time 10 s. Human fibroblasts growing in a monolayer on a cover slide were exposed to microwave electromagnetic radiation. The layer of medium that covered cells during microwave exposure was about 1 mm thick. Cells were stained immediately after irradiation by 2% (w/v) orcein solution in 45% (w/v) acetic acid. Experiments were made at room temperature (25 °C), and control cell samples were processed in the same conditions. We assessed heterochromatin granule quantity (HGQ) at 600× magnification. Microwave irradiation at the intensity of 1 µW/cm(2) produced no effect, and irradiation at the intensities of 10 and 100 µW/cm(2) induced an increase in HGQ. More intense irradiation induced more chromatin condensation. The right-handed elliptically polarized radiation revealed more biological activity than the left-handed polarized one.
authors.affiliation: Kharkiv National University, pl. Svobody 4, Kharkiv 61077, Ukraine. Yury.G.Shckorbatov@univer.kharkov.ua
authors.forename: Yuriy G
authors.initials: YG
authors.lastname: Shckorbatov
authors.forename: Vladimir N
authors.initials: VN
authors.lastname: Pasiuga
authors.forename: Elena I
authors.initials: EI
authors.lastname: Goncharuk
authors.forename: Tatiana Ph
authors.initials: TP
authors.lastname: Petrenko
authors.forename: Valentin A
authors.initials: VA
authors.lastname: Grabina
authors.forename: Nicolay N
authors.initials: NN
authors.lastname: Kolchigin
authors.forename: Dmitry D
authors.initials: DD
authors.lastname: Ivanchenko
authors.forename: Victor N
authors.initials: VN
authors.lastname: Bykov
authors.forename: Oleksandr M
authors.initials: OM
authors.lastname: Dumin
mesh_terms: D002467:Cell Nucleus / Q000528:radiation effects* / Q000648:ultrastructure; D002478:Cells, Cultured; D005347:Fibroblasts / Q000528:radiation effects* / Q000648:ultrastructure; D006570:Heterochromatin / Q000528:radiation effects; D006801:Humans; D008872:Microwaves
chemical_list: D006570:Heterochromatin