title: Identification of a red-emitting fluorescent ligand for in vitro visualization of human serotonin 5-HT(1A) receptors.
abstract: The 5-HT(1A) receptor subtype is the most thoroughly studied serotonin receptor subtype. We report here the design, synthesis and characterization of two new fluorescent ligands for the 5-HT(1A) receptor. The new 1-arylpiperazine-based red-emitting fluorescent compound 6 displayed good binding affinity at the 5-HT(1A) receptor (K(i)=35 nM) and was able to label specifically the human 5-HT(1A) receptor stably expressed in CHO cells visualized using confocal laser scanning microscopy.
authors.affiliation: Università degli Studi di Bari A. Moro, Dipartimento Farmaco-Chimico, via Orabona, 4, 70125 Bari, Italy.
authors.forename: Enza
authors.initials: E
authors.lastname: Lacivita
authors.forename: Anna Carmela
authors.initials: AC
authors.lastname: Masotti
authors.forename: Md
authors.initials: M
authors.lastname: Jafurulla
authors.forename: Roopali
authors.initials: R
authors.lastname: Saxena
authors.forename: Nandini
authors.initials: N
authors.lastname: Rangaraj
authors.forename: Amitabha
authors.initials: A
authors.lastname: Chattopadhyay
authors.forename: Nicola A
authors.initials: NA
authors.lastname: Colabufo
authors.forename: Francesco
authors.initials: F
authors.lastname: Berardi
authors.forename: Roberto
authors.initials: R
authors.lastname: Perrone
authors.forename: Marcello
authors.initials: M
authors.lastname: Leopoldo
mesh_terms: D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D008024:Ligands; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D044282:Receptor, Serotonin, 5-HT1A / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D008024:Ligands; D044282:Receptor, Serotonin, 5-HT1A