title: Protection of sheep against Rift Valley fever virus and sheep poxvirus with a recombinant capripoxvirus vaccine.
abstract: Rift Valley fever (RVF) is an epizootic viral disease of sheep that can be transmitted from sheep to humans, particularly by contact with aborted fetuses. A capripoxvirus (CPV) recombinant virus (rKS1/RVFV) was developed, which expressed the Rift Valley fever virus (RVFV) Gn and Gc glycoproteins. These expressed glycoproteins had the correct size and reacted with monoclonal antibodies (MAb) to native glycoproteins. Mice vaccinated with rKS1/RVFV were protected against RVFV challenge. Sheep vaccinated with rKS1/RVFV twice developed neutralizing antibodies and were significantly protected against RVFV and sheep poxvirus challenge. These findings further document the value of CPV recombinants as ruminant vaccine vectors and support the inclusion of RVFV genes encoding glycoproteins in multivalent recombinant vaccines to be used where RVF occurs.
authors.affiliation: Kenya Agricultural Research Institute, P.O. Box 57811-00200, Nairobi, Kenya.
authors.forename: Reuben K
authors.initials: RK
authors.lastname: Soi
authors.forename: Fred R
authors.initials: FR
authors.lastname: Rurangirwa
authors.forename: Travis C
authors.initials: TC
authors.lastname: McGuire
authors.forename: Paul M
authors.initials: PM
authors.lastname: Rwambo
authors.forename: James C
authors.initials: JC
authors.lastname: DeMartini
authors.forename: Timothy B
authors.initials: TB
authors.lastname: Crawford
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood; D018151:Capripoxvirus / Q000235:genetics / Q000276:immunology*; D004195:Disease Models, Animal; D005260:Female; D008297:Male; D051379:Mice; D011213:Poxviridae Infections / Q000517:prevention & control / Q000662:veterinary*; D012295:Rift Valley Fever / Q000517:prevention & control / Q000662:veterinary*; D012296:Rift Valley fever virus / Q000235:genetics / Q000276:immunology*; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology / Q000517:prevention & control*; D014614:Vaccines, Synthetic / Q000235:genetics / Q000276:immunology; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology*
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D014614:Vaccines, Synthetic; D014765:Viral Vaccines