title: Platelet-rich plasma on calcium phosphate granules promotes metaphyseal bone healing in mini-pigs.
abstract: The role of platelet-rich plasma (PRP) as a promoter of bone healing remains controversial. The aim of this study was to investigate the effect of PRP in combination with calcium phosphate granules (CPG) on bone defect healing in a metaphyseal long bone defect. A metaphyseal bone defect at the proximal tibia of 16 mini-pigs was filled with CPG combined with autologous PRP or CPG solely (control group). The PRP showed 4.4-fold more platelets compared to peripheral blood. Six weeks after surgery the radiological and histomorphometrical evaluations showed significantly more bone formation in the PRP group in the central area of the defect zone (p < 0.01) as well as the cortical defect zone (p < 0.04). Furthermore, the resorption rate of CPG was increased in animals who received PRP. Nevertheless there were only isolated instances of complete osseous bridging of the bone defects even in the PRP group. This study demonstrates that a PRP-CPG composit promotes bone regeneration but does not lead to a solid fusion of a tibial defect in mini-pigs.
authors.affiliation: Department of Trauma and Handsurgery, Heinrich Heine University Hospital Duesseldorf, Moorenstr 5, 40225 Duesseldorf, Germany.
authors.forename: Pascal
authors.initials: P
authors.lastname: Jungbluth
authors.forename: Michael
authors.initials: M
authors.lastname: Wild
authors.forename: Jan-Peter
authors.initials: JP
authors.lastname: Grassmann
authors.forename: Ebru
authors.initials: E
authors.lastname: Ar
authors.forename: Martin
authors.initials: M
authors.lastname: Sager
authors.forename: Monika
authors.initials: M
authors.lastname: Herten
authors.forename: Marcus
authors.initials: M
authors.lastname: Jäger
authors.forename: Juergen
authors.initials: J
authors.lastname: Becker
authors.forename: Joachim
authors.initials: J
authors.lastname: Windolf
authors.forename: Mohssen
authors.initials: M
authors.lastname: Hakimi
mesh_terms: D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects*; D002130:Calcium Phosphates / Q000494:pharmacology*; D005260:Female; D010012:Osteogenesis / Q000187:drug effects; D053657:Platelet-Rich Plasma / Q000502:physiology*; D013552:Swine; D013556:Swine, Miniature
chemical_list: D002130:Calcium Phosphates; C020243:calcium phosphate