title: Surface enhanced Raman evidence for Ag+ complexes of adenine, deoxyadenosine and 5'-dAMP formed in silver colloids.
abstract: We report the formation of highly scattering silver complexes of adenine, deoxyadenosine and 5'-dAMP under alkaline pH conditions in the colloidal silver solutions which are used for surface-enhanced Raman spectroscopy. These complexes, and other pH-dependent phenomena, help to explain the diversity of previously reported adenine SERS spectra. Using conditions which promote complex formation allows nucleotides to be detected at <1 ppm, even in solutions with high salt concentrations.
authors.affiliation: Innovative Molecular Materials Group, School of Chemistry and Chemical Engineering, Queen's University, Belfast, UK BT9 5AG.
authors.forename: Evanthia
authors.initials: E
authors.lastname: Papadopoulou
authors.forename: Steven E J
authors.initials: SE
authors.lastname: Bell
mesh_terms: D000225:Adenine / Q000737:chemistry*; D000249:Adenosine Monophosphate / Q000737:chemistry*; D003102:Colloids / Q000737:chemistry*; D003839:Deoxyadenosines / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D015394:Molecular Structure; D012492:Salts / Q000737:chemistry; D012834:Silver / Q000737:chemistry*; D018030:Silver Compounds / Q000737:chemistry*; D013059:Spectrum Analysis, Raman / Q000379:methods*; D013499:Surface Properties
chemical_list: D003102:Colloids; D003839:Deoxyadenosines; D012492:Salts; D018030:Silver Compounds; D012834:Silver; D000249:Adenosine Monophosphate; D000225:Adenine