An enzyme-free highly glucose-specific assay using self-assembled aminobenzene boronic acid upon polyelectrolytes electrospun nanofibers-mat.
A highly selective enzyme-free amperometric glucose sensor based on electrostatic self-assembling of 3-aminobenzene boronic acid (ABBA) onto a poly(styrene-co-acrylamide)/polystyrene sulfonic acid (PSA/PSSA) electrospun nanofibers-mat was investigated. Emerging ability of phenylboronic acid to bind with the diols of sugars has been extended for rapid response of glucose with a pH-sensitive redox mediator, hematein natural dye. ABBA was adsorbed on the PSA/PSSA nanofibers-mat/Pt-disc electrode that resulted in an ABBA/PSA/PSSA glucose active electrode. The interaction of ABBA onto the PSA/PSSA nanofibers-mat/Pt-disc electrode was characterized with Fourier transform infrared spectroscopy (FT-IR), ζ-potential, scanning electron microscopy (SEM), contact angle and cyclic voltammetry (CV) measurements. The prepared enzyme-free sensor exhibited a fast amperometric response, i.e., about 4s and linearity ranging from 0.75 to 14mM to glucose with a sensitivity of 0.987μAmM(-1)cm(-2). Compared to other types of glucose biosensors viz. use glucose oxidase as sensing elements, present glucose sensor offers basic advantages including ease of fabrication, high affinity-selectivity to the glucose upon the electrode surface and quick response.
Tiwari
Ashutosh
A
Biomaterials Center, National Institute for Materials Science, 1-2-1 Sengen, Tsukuba, Ibaraki 350047, Japan. tiwari.ashutosh@nims.go.jp
Terada
Dohiko
D
Yoshikawa
Chiaki
C
Kobayashi
Hisatoshi
H
D000180:Acrylic Resins / Q000138:chemical synthesis / Q000737:chemistry*; D001786:Blood Glucose / Q000032:analysis; D001897:Boronic Acids / Q000737:chemistry*; D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods*; D004573:Electrolytes / Q000138:chemical synthesis / Q000737:chemistry*; D005947:Glucose / Q000032:analysis*; D015394:Molecular Structure; D057139:Nanofibers / Q000737:chemistry*; D011137:Polystyrenes / Q000138:chemical synthesis / Q000737:chemistry*; D012680:Sensitivity and Specificity; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D013501:Surface-Active Agents / Q000138:chemical synthesis / Q000737:chemistry; D013997:Time Factors
D000180:Acrylic Resins; D001786:Blood Glucose; D001897:Boronic Acids; D004573:Electrolytes; D011137:Polystyrenes; D013501:Surface-Active Agents; C555360:poly(styrene-co-acrylamide); C028592:3-aminobenzeneboronic acid; C003321:polystyrene sulfonic acid; D005947:Glucose