title: [Functional analysis of the HERV-K LTR residing in the KIAA1245/NBPF subfamily].
abstract: Long terminal repeats (LTRs) of human endogenous retrovihuses (HERs) might affect transcription regulation of neighboring genes. In our previous study, we showed that the solitary LTR residing in the KIAA1245/NBPF gene subfamily displayed high enhancer activity in a transformed embryonal carcinoma cell line Tera1. In this study, we performed a functional dissection of the LTR and studied its deletion series. Using transient transfection assay, we confirmed the ability of the LTR to drive the expression of the luciferase reporter gene in Teral cells. At the same time, in two other transformed cell lines tested, NGP and NT2/D1, the full-size LTR and its fragments showed no or low enhancer activity, thus demonstrating cell type specificity of the LTR enhancer activity. The functional dissection of the LTRrevealed a specific region within the U3 part appeared to be responsible for the enhancer properties. We showed that the identified enhancer was able to work in a highly cell type specific manner. The data obtained are in line with the hypothesis suggesting that KIAA1245/NBPF LTR may affect the regulation of the KIAA1245/NBPF subfamily genes transcription.
authors.affiliation: Shemy-akin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997, Russia. natalya_abrarova@mail.ru
authors.forename: N D
authors.initials: ND
authors.lastname: Abrarova
authors.forename: E A
authors.initials: EA
authors.lastname: Stukacheva
authors.forename: V V
authors.initials: VV
authors.lastname: Pleshkan
authors.forename: T V
authors.initials: TV
authors.lastname: Vinogradova
authors.forename: E D
authors.initials: ED
authors.lastname: Sverdlov
mesh_terms: D002352:Carrier Proteins / Q000096:biosynthesis* / Q000235:genetics; D045744:Cell Line, Tumor; D020077:Endogenous Retroviruses / Q000235:genetics / Q000378:metabolism*; D004742:Enhancer Elements, Genetic / Q000502:physiology*; D006801:Humans; D005810:Multigene Family / Q000502:physiology*; D020079:Terminal Repeat Sequences / Q000502:physiology*; D014158:Transcription, Genetic / Q000502:physiology*
chemical_list: D002352:Carrier Proteins