title: Gene expression profiling reveals novel biomarkers in nonsmall cell lung cancer.
abstract: The development of reliable gene expression profiling technology is having an increasing impact on our understanding of lung cancer biology. Our study aimed to determine any correlation between the phenotypic heterogeneity and genetic diversity of lung cancer. Microarray analysis was performed on a set of 46 tumor samples and 45 paired nontumor samples of nonsmall cell lung cancer (NSCLC) samples to establish gene signatures in primary adenocarcinomas and squamous-cell carcinomas, determine differentially expressed gene sequences at different stages of the disease and identify sequences with biological significance for tumor progression. After the microarray analysis, the expression level of 92 selected genes was validated by qPCR and the robust Bonferroni test in an independent set of 70 samples composed of 48 tumor samples and 22 nontumor samples. Gene sequences were differentially expressed as a function of tumor type, stage and differentiation grade. High upregulation was observed for KRT15 and PKP1, which may be good markers to distinguish squamous-cell carcinoma samples. High downregulation was observed for DSG3 in stage IA adenocarcinomas.
authors.affiliation: Department of Thoracic Surgery, Virgen de las Nieves University Hospital, Granada, Spain.
authors.forename: Abel
authors.initials: A
authors.lastname: Sanchez-Palencia
authors.forename: Mercedes
authors.initials: M
authors.lastname: Gomez-Morales
authors.forename: Jose Antonio
authors.initials: JA
authors.lastname: Gomez-Capilla
authors.forename: Vicente
authors.initials: V
authors.lastname: Pedraza
authors.forename: Laura
authors.initials: L
authors.lastname: Boyero
authors.forename: Rafael
authors.initials: R
authors.lastname: Rosell
authors.forename: M Esther
authors.initials: ME
authors.lastname: Fárez-Vidal
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D014408:Biomarkers, Tumor / Q000235:genetics; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000473:pathology; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D016022:Case-Control Studies; D020869:Gene Expression Profiling; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D009367:Neoplasm Staging; D015203:Reproducibility of Results
chemical_list: D014408:Biomarkers, Tumor