title: Monitoring of Bordetella isolates circulating in Saint Petersburg, Russia between 2001 and 2009.
abstract: Bordetella isolates in the Saint Petersburg region have been monitored since 1998. Over the past ten years, concomitant with the increase in pertussis whole-cell vaccine coverage, the incidence of whooping cough has decreased. However, this decrease exists only for Bordetella pertussis infections, as the incidence of Bordetella parapertussis confirmed cases has remained stable, suggesting that pertussis-vaccine-induced immunity is not protective against parapertussis, as expected. B. pertussis and B. parapertussis clinical isolates were analyzed using serotyping, immunoblotting, pulsed-field gel electrophoresis of chromosomal DNA (after digestion with XbaI) and sequencing of virulence genes. The bacterial population is now similar to that observed in other European countries.
authors.affiliation: Institut Pasteur Epidemiology and Microbiology-Bacterial Respiratory Infections Laboratory, 14, Mira street, 197101 St. Petersburg, Russia.
authors.forename: Natalia
authors.initials: N
authors.lastname: Kurova
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Njamkepo
authors.forename: Delphine
authors.initials: D
authors.lastname: Brun
authors.forename: Galina
authors.initials: G
authors.lastname: Tseneva
authors.forename: Nicole
authors.initials: N
authors.lastname: Guiso
mesh_terms: D015373:Bacterial Typing Techniques; D001885:Bordetella Infections / Q000453:epidemiology* / Q000382:microbiology*; D042483:Bordetella parapertussis / Q000145:classification* / Q000302:isolation & purification*; D001886:Bordetella pertussis / Q000145:classification* / Q000302:isolation & purification*; D002648:Child; D002675:Child, Preschool; D016000:Cluster Analysis; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D015151:Immunoblotting; D015994:Incidence; D007223:Infant; D017720:Molecular Epidemiology; D058889:Molecular Typing; D012426:Russia / Q000453:epidemiology; D017422:Sequence Analysis, DNA; D012703:Serotyping; D037521:Virulence Factors / Q000235:genetics
chemical_list: D037521:Virulence Factors