Successful live birth from vitrified oocytes after 5 years of cryopreservation.
OBJECTIVE
To report a birth of a healthy boy after long-term cryopreservation of oocytes by vitrification.


METHODS
Clinical application.


METHODS
IVF Center.


METHODS
A 17 year-old female with secondary pulmonary hypertension caused by transposition of great vessels visited our center in 2002, and she wished oocytes cryopreservation to avoid possible sterility after the following category X medication treatment.


METHODS
Vitrified oocytes on Electron Microscope (EM) grids were warmed after 5 years of storage. Surviving MII oocytes were microinjected for fertilization and two embryos were transferred into a gestational carrier day 5 after microinjection.


METHODS
Survival, fertilization, cleavage, clinical pregnancy and delivery.


RESULTS
Eleven out of fourteen oocytes (78.6%) survived warming. Eight Metaphase II (MII) oocytes and 3 in vitro matured oocytes were microinjected; all 11 oocytes (100%) fertilized and 2 embryos were transferred on day 5. A healthy baby boy weighing 3,600 g was delivered at 38 weeks of gestation. Live-birth rates per warmed oocyte and per injected oocyte were 7.1% and 9.1% respectively.


CONCLUSIONS
Cryopreservation after vitrification with EM grids maintained the developmental competence of oocytes after long-term storage and resulted in a successful live birth.
Kim
Thomas J
TJ
Reproductive Medicine Associates of New Jersey, Morristown, NJ, 07960, USA.
Hong
Seung Wook
SW
D000293:Adolescent; D015925:Cryopreservation; D004624:Embryo Transfer; D047108:Embryonic Development; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D007223:Infant; D050498:Live Birth; D008297:Male; D008677:Metaphase; D009865:Oocytes / Q000254:growth & development*; D011247:Pregnancy; D013997:Time Factors; D016896:Treatment Outcome; D058989:Vitrification; D055815:Young Adult