title: Co-detection: ultra-reliable nanoparticle-based electrical detection of biomolecules in the presence of large background interference.
abstract: An ultra-reliable technique for detecting trace quantities of biomolecules is reported. The technique called "co-detection" exploits the non-linear redundancy amongst synthetically patterned biomolecular logic circuits for deciphering the presence or absence of target biomolecules in a sample. In this paper, we verify the "co-detection" principle on gold-nanoparticle-based conductimetric soft-logic circuits which use a silver-enhancement technique for signal amplification. Using co-detection, we have been able to demonstrate a great improvement in the reliability of detecting mouse IgG at concentration levels that are 10(5) lower than the concentration of rabbit IgG which serves as background interference.
authors.affiliation: Department of Civil and Environmental Engineering, The University of Michigan, USA. yaliu@umich.edu
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Ming
authors.initials: M
authors.lastname: Gu
authors.forename: Evangelyn C
authors.initials: EC
authors.lastname: Alocilja
authors.forename: Shantanu
authors.initials: S
authors.lastname: Chakrabartty
mesh_terms: D000818:Animals; D000888:Antibodies, Anti-Idiotypic; D055910:Antibodies, Immobilized; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods* / Q000706:statistics & numerical data; D055664:Electrochemical Techniques; D006046:Gold; D007074:Immunoglobulin G / Q000032:analysis; D053768:Metal Nanoparticles; D051379:Mice; D011817:Rabbits; D015203:Reproducibility of Results; D012834:Silver
chemical_list: D000888:Antibodies, Anti-Idiotypic; D055910:Antibodies, Immobilized; D007074:Immunoglobulin G; D012834:Silver; D006046:Gold