title: Optogenetic control of heart muscle in vitro and in vivo.
abstract: Electrical stimulation is the standard technique for exploring electrical behavior of heart muscle, but this approach has considerable technical limitations. Here we report expression of the light-activated cation channel channelrhodopsin-2 for light-induced stimulation of heart muscle in vitro and in mice. This method enabled precise localized stimulation and constant prolonged depolarization of cardiomyocytes and cardiac tissue resulting in alterations of pacemaking, Ca(2+) homeostasis, electrical coupling and arrhythmogenic spontaneous extrabeats.
authors.affiliation: Institute of Physiology I, Life and Brain Center, University of Bonn, Bonn, Germany.
authors.forename: Tobias
authors.initials: T
authors.lastname: Bruegmann
authors.forename: Daniela
authors.initials: D
authors.lastname: Malan
authors.forename: Michael
authors.initials: M
authors.lastname: Hesse
authors.forename: Thomas
authors.initials: T
authors.lastname: Beiert
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Fuegemann
authors.forename: Bernd K
authors.initials: BK
authors.lastname: Fleischmann
authors.forename: Philipp
authors.initials: P
authors.lastname: Sasse
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D000075402:Channelrhodopsins; D004558:Electric Stimulation; D004562:Electrocardiography; D051379:Mice; D032383:Myocytes, Cardiac / Q000502:physiology*; D010138:Pacemaker, Artificial
chemical_list: D000075402:Channelrhodopsins; D002118:Calcium