Characterization of the Streptococcus sobrinus acid-stress response by interspecies microarrays and proteomics.
Streptococcus mutans and Streptococcus sobrinus are considered the primary organisms responsible for human dental caries. The ability to generate acids and to adapt to low pH conditions is directly associated with the cariogenic potential of these bacteria. To survive acidic conditions, both species have been shown to mount an acid-tolerance response (ATR). However, previous characterization of the S. sobrinus ATR identified critical differences in the mechanisms of acid adaptation between S. mutans and S. sobrinus. Here, interspecies microarray and proteomic approaches were used to identify novel, previously unrecognized genes and pathways that participate in the S. sobrinus acid-stress response. The results revealed that, among other things, metabolic alterations that enhance energy generation and upregulation of the malolactic fermentation enzyme activity constitute important acid-resistance properties in S. sobrinus. Some of these acid adaptive traits are shared by S. mutans and might be considered optimal targets for therapeutic treatments designed to control dental caries.
Martinez
A R
AR
Center for Oral Biology, University of Rochester Medical Center, Rochester, NY 14642, USA.
Abranches
J
J
Kajfasz
J K
JK
Lemos
J A
JA
D000222:Adaptation, Physiological / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics*; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D006019:Glycolysis / Q000235:genetics*; D006863:Hydrogen-Ion Concentration; D019344:Lactic Acid / Q000378:metabolism; D008291:Malate Dehydrogenase / Q000235:genetics* / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D013045:Species Specificity; D013295:Streptococcus mutans / Q000235:genetics / Q000378:metabolism; D016986:Streptococcus sobrinus / Q000235:genetics* / Q000378:metabolism*; D053719:Tandem Mass Spectrometry
D001426:Bacterial Proteins; D019344:Lactic Acid; C037731:malolactic enzyme; D008291:Malate Dehydrogenase