Involvement of gicerin, a cell adhesion molecule, in a dermal autograft chicken model.
Gicerin is a cell adhesion molecule in the immunoglobulin superfamily. This molecule has homophilic and heterophilic adhesive activities, binding to the neurite out-growth factor (NOF). We have previously reported that gicerin plays an important role in the development and regeneration as well as in the metastasis of tumors through its adhesive activities, mediating cell-cell and/or cell-extracellular matrix interactions. In this study, we investigated the involvement of gicerin in a dermal autograft chicken model. Gicerin and NOF were transiently present in the regenerating epithelia after the dermal graft transplantation. The treatment with an anti-gicerin polyclonal antibody, by placing drops onto the wounds, inhibited the adhesiveness of the grafts to the marginal skin. The chimeric protein of gicerin-IgG, gicerin-Fc, and NOF proteins promoted the regeneration of the grafts. These findings suggest the potential function of gicerin in dermal autografts, and gicerin and NOF proteins could help clinical improvement after transplantations.
Tsukamoto
Yasuhiro
Y
Department of Animal Hygiene, Graduate School of Biology and Environmental Sciences, Kyoto Prefecture University, Kyoto 606-8522, Japan. ytsuka@kpu.ac.jp
Kataoka
Bin
B
Adachi
Kazuhide
K
Kato
Hidenori
H
Inai
Marie
M
Tsukamoto
Masaya
M
Handharyani
Ekowati
E
Taira
Eiichi
E
D000818:Animals; D000906:Antibodies / Q000378:metabolism; D030161:Avian Proteins / Q000378:metabolism*; D051929:CD146 Antigen / Q000378:metabolism*; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D002645:Chickens; D020405:Dermis / Q000637:transplantation*; D004848:Epithelium / Q000378:metabolism; D023421:Models, Animal; D009414:Nerve Growth Factors / Q000378:metabolism; D012038:Regeneration; D014182:Transplantation, Autologous
D000906:Antibodies; D030161:Avian Proteins; D051929:CD146 Antigen; D015815:Cell Adhesion Molecules; D009414:Nerve Growth Factors; C086935:gicerin protein, Gallus gallus