title: Differential receptor binding affinities of influenza hemagglutinins on glycan arrays.
abstract: A library of 27 sialosides, including seventeen 2,3-linked and ten 2,6-linked glycans, has been prepared to construct a glycan array and used to profile the binding specificity of different influenza hemagglutinins (HA) subtypes, especially from the 2009 swine-originated H1N1 and seasonal influenza viruses. It was found that the HAs from the 2009 H1N1 and the seasonal Brisbane strain share similar binding profiles yet different binding affinities toward various α2,6 sialosides. Analysis of the binding profiles of different HA subtypes indicate that a minimum set of 5 oligosaccharides can be used to differentiate influenza H1, H3, H5, H7, and H9 subtypes. In addition, the glycan array was used to profile the binding pattern of different influenza viruses. It was found that most binding patterns of viruses and HA proteins are similar and that glycosylation at Asn27 is essential for receptor binding.
authors.affiliation: Genomics Research Center, Academia Sinica, 128 Academia Road, Section 2, Nankang, Taipei 115, Taiwan.
authors.forename: Hsin-Yu
authors.initials: HY
authors.lastname: Liao
authors.forename: Che-Hsiung
authors.initials: CH
authors.lastname: Hsu
authors.forename: Shih-Chi
authors.initials: SC
authors.lastname: Wang
authors.forename: Chi-Hui
authors.initials: CH
authors.lastname: Liang
authors.forename: Hsin-Yung
authors.initials: HY
authors.lastname: Yen
authors.forename: Ching-Yao
authors.initials: CY
authors.lastname: Su
authors.forename: Chien-Hung
authors.initials: CH
authors.lastname: Chen
authors.forename: Jia-Tsrong
authors.initials: JT
authors.lastname: Jan
authors.forename: Chien-Tai
authors.initials: CT
authors.lastname: Ren
authors.forename: Chung-Hsuan
authors.initials: CH
authors.lastname: Chen
authors.forename: Ting-Jen R
authors.initials: TJ
authors.lastname: Cheng
authors.forename: Chung-Yi
authors.initials: CY
authors.lastname: Wu
authors.forename: Chi-Huey
authors.initials: CH
authors.lastname: Wong
mesh_terms: D002240:Carbohydrate Sequence; D002460:Cell Line; D006031:Glycosylation; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000378:metabolism*; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000378:metabolism*; D011134:Polysaccharides / Q000378:metabolism*; D011485:Protein Binding; D011991:Receptors, Virus / Q000378:metabolism*
chemical_list: D019267:Hemagglutinin Glycoproteins, Influenza Virus; D011134:Polysaccharides; D011991:Receptors, Virus