The use of cytokine array to examine cytokine profiles of two human cell lines exposed to indoor dust.
Human cytokine array was used to investigate the cytokine profile of U937 and KERTr after exposure to indoor dust or dust extracts. The release of MCP-1 was increased while release of IL-8 and IL-1β on U937 were decreased after exposure to indoor dust. The releases of RANTES, IL-8 and VEGF from KERTr after exposure to dust extract were increased. The results of IL-8 ELISA assay were consistent with the cytokine array. Real-time RT-PCR was performed to analyze relative changes in gene expression. The MCP-1 mRNA levels were increased after U937 exposure to 18 indoor dust samples, whereas, IL-8 and IL-1β mRNA level showed both up-regulation and down-regulation. The dose-related increase and decrease response was observed on MCP-1 and IL-8, respectively. Most indoor dust extracts increased RANTES, IL-8 and VEGF mRNA levels on KERTr. The dose-dependent response was observed on RANTES and IL-8. A significant correlation (r=0.48, p<0.05) was obtained between the total PAHs concentration in dust extracts and the induction of RANTES mRNA.
Kang
Yuan
Y
Croucher Institute for Environmental Sciences, Department of Biology, Hong Kong Baptist University, Kowloon Tong, Hong Kong, PR China.
Cheung
Kwai Chung
KC
Wong
Ming H
MH
D016902:Air Pollution, Indoor / Q000009:adverse effects*; D016207:Cytokines / Q000032:analysis* / Q000235:genetics; D004391:Dust; D004797:Enzyme-Linked Immunosorbent Assay; D005786:Gene Expression Regulation; D006801:Humans; D015603:Keratinocytes / Q000276:immunology; D012333:RNA, Messenger / Q000032:analysis; D013172:Spores, Fungal; D020298:U937 Cells
D016207:Cytokines; D004391:Dust; D012333:RNA, Messenger