Biocompatibility evaluation of HDPE-UHMWPE reinforced β-TCP nanocomposites using highly purified human osteoblast cells.
Biocompatibility of β-TCP/HDPE-UHMWPE nanocomposite as a new bone substitute material was evaluated by using highly purified human osteoblast cells. Human osteoblast cells were isolated from bone tissue and characterized by immunofluorescence Staining before and after purification using magnetic bead system. Moreover, proliferation, alkaline phosphatase production, cell attachment, calcium deposition, gene expression, and morphology of osteoblast cells on β-TCP/HDPE-UHMWPE nanocomposites were evaluated. The results have shown that the human osteoblast cells were successfully purified and were suitable for subsequent cell culturing process. The high proliferation rate of osteoblast cells on β-TCP/HDPE-UHMWPE nanocomposite confirmed the great biocompatibility of the scaffold. Expression of bone-specific genes was taken place after the cells were incubated in composite extract solutions. Furthermore, osteoblast cells were able to mineralize the matrix next to composite samples. Scanning electron microscopy demonstrated that cells had normal morphology on the scaffold. Thus, these results indicated that the nanosized β-TCP/HDPE-UHMWPE blend composites could be potential scaffold, which is used in bone tissue engineering.
Shokrgozar
M A
MA
National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran. mashokrgozar@pasteur.ac.ir
Farokhi
M
M
Rajaei
F
F
Bagheri
M H A
MH
Azari
Sh
Sh
Ghasemi
I
I
Mottaghitalab
F
F
Azadmanesh
K
K
Radfar
J
J
D000469:Alkaline Phosphatase / Q000378:metabolism; D001672:Biocompatible Materials / Q000494:pharmacology*; D002130:Calcium Phosphates / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D002469:Cell Separation; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D008422:Materials Testing / Q000379:methods*; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology / Q000648:ultrastructure; D010316:Particle Size; D020959:Polyethylene / Q000494:pharmacology*; D011095:Polyethylenes / Q000494:pharmacology*; D011208:Powders; D013194:Staining and Labeling; D014961:X-Ray Diffraction
D001672:Biocompatible Materials; D002130:Calcium Phosphates; D011095:Polyethylenes; D011208:Powders; C485817:beta-tricalcium phosphate; C111601:ultra-high molecular weight polyethylene; D020959:Polyethylene; D000469:Alkaline Phosphatase