title: Improved isoflavonoid production in Pueraria candollei hairy root cultures using elicitation.
abstract: The effect of abiotic and biotic elicitors (methyl jasmonate, chitosan, salicylic acid, Agrobacterium, and yeast extract) at various concentrations on total isoflavonoid accumulation was studied in the hairy root cultures of Pueraria candollei. All elicitors stimulated isoflavonoid production. Yeast extract (0.5 mg/ml) was the most efficient giving total isoflavonoids at 60 ± 1 mg/g dry wt, which was 4.5-fold higher than control hairy roots on day 3 of elicitation.
authors.affiliation: Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand.
authors.forename: Latiporn
authors.initials: L
authors.lastname: Udomsuk
authors.forename: Kanokwan
authors.initials: K
authors.lastname: Jarukamjorn
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Tanaka
authors.forename: Waraporn
authors.initials: W
authors.lastname: Putalun
mesh_terms: D001709:Biotechnology / Q000379:methods*; D003470:Culture Media / Q000737:chemistry; D007529:Isoflavones / Q000378:metabolism*; D018517:Plant Roots / Q000378:metabolism*; D029908:Pueraria / Q000378:metabolism*
chemical_list: D003470:Culture Media; D007529:Isoflavones