Successful transplant of mesenchymal stem cells in induced osteonecrosis of the ovine femoral head: preliminary results.
OBJECTIVE
Evaluate the bone tissue recovery following transplantation of ovine mesenchymal stem cells (MSC) from bone marrow and human immature dental-pulp stem cells (hIDPSC) in ovine model of induced osteonecrosis of femoral head (ONFH).


METHODS
Eight sheep were divided in three experimental groups. First group was composed by four animals with ONFH induced by ethanol through central decompression (CD), for control group without any treatment. The second and third group were compose by two animals, six weeks after ONFH induction received transplantation of heterologous ovine MSC (CD + oMSC), and hIDPSC (CD + hIDPSC), respectively. In both experiments the cells were transplanted without application of any type of immunosupression protocol.


RESULTS
Our data indicate that both cell types used in experiments were able to proliferate within injured site providing bone tissue recovery. The histological results obtained from CD+hIDPSC suggested that the bone regeneration in such animals was better than that observed in CD animals.


CONCLUSIONS
Mesenchymal stem cell transplant in induced ovine osteonecrosis of femoral head by central decompression technique is safe, and apparently favors bone regeneration of damaged tissues.
Feitosa
Matheus Levi Tajra
ML
Department of Surgery, Faculty of Veterinary Medicine and Animal Science, USP, Sao Paulo, Brazil. mtajra@gmail.com
Fadel
Leandro
L
Beltrão-Braga
Patrícia Cristina Baleeiro
PC
Wenceslau
Cristiane Valverde
CV
Kerkis
Irina
I
Kerkis
Alexandre
A
Birgel Júnior
Eduardo Harry
EH
Martins
João Flávio Panattoni
JF
Martins
Daniele dos Santos
Ddos S
Miglino
Maria Angélica
MA
Ambrósio
Carlos Eduardo
CE
D000818:Animals; D001672:Biocompatible Materials / Q000009:adverse effects; D003782:Dental Pulp / Q000637:transplantation*; D004195:Disease Models, Animal; D005271:Femur Head Necrosis / Q000601:surgery*; D006801:Humans; D045164:Mesenchymal Stem Cell Transplantation / Q000009:adverse effects; D011897:Random Allocation; D012756:Sheep; D014183:Transplantation, Heterologous
D001672:Biocompatible Materials