title: Integration of scale-down experimentation and general rate modelling to predict manufacturing scale chromatographic separations.
abstract: Chromatography is an essential downstream processing step in the production of biopharmaceuticals. Here we present an approach to chromatography scale-up using scale-down experimentation integrated with general rate modelling. This type of modelling takes account all contributions to the mass transfer kinetics providing process understanding. The model is calibrated using a 2.5 cm height, 1 ml column and used to predict chromatograms for 20 cm height columns from 40 ml to 160 L volume. Simulations were found to be in good agreement with experimental results. The envisaged approach could potentially reduce the number of experiments, shorten development time and reduce costs.
authors.affiliation: The Advanced Centre for Biochemical Engineering, Department of Biochemical Engineering, University College London, Torrington Place, London WC1 7JE, UK.
authors.forename: Spyridon
authors.initials: S
authors.lastname: Gerontas
authors.forename: Magnus
authors.initials: M
authors.lastname: Asplund
authors.forename: Rolf
authors.initials: R
authors.lastname: Hjorth
authors.forename: Daniel G
authors.initials: DG
authors.lastname: Bracewell
mesh_terms: D000465:Algorithms; D002852:Chromatography, Ion Exchange / Q000379:methods*; D003198:Computer Simulation; D007475:Ion Exchange Resins / Q000737:chemistry*; D007700:Kinetics; D007781:Lactoferrin / Q000302:isolation & purification; D008962:Models, Theoretical; D015203:Reproducibility of Results; D012710:Serum Albumin, Bovine / Q000302:isolation & purification
chemical_list: D007475:Ion Exchange Resins; D012710:Serum Albumin, Bovine; D007781:Lactoferrin