title: Relation between fluorescence spectra of dilute and turbid samples.
abstract: We present a method to extend rank-annihilation-factor analysis (RAFA) for the analysis of fluorescence from homogeneous turbid samples. The method is based on a fundamental relationship between the fluorescence of a dilute solution and that of a turbid solution. We have derived this relationship, known as the transfer function, for turbid materials using the two-flux Kubelka-Munk theory. The method is tested with spectroscopic data from optically thin and turbid samples of the media of a human aorta. At 450-nm excitation, agreement between the measured and predicted dilute-solution fluorescence spectra is within 5% at all emission wavelengths; at 340-nm excitation, agreement is within 20% at all wavelengths, with some residual Soret-band absorption. The simulations presented indicate that the transfer function is markedly more sensitive to absorption than to scattering properties.
authors.forename: A J
authors.initials: AJ
authors.lastname: Durkin
authors.forename: S
authors.initials: S
authors.lastname: Jaikumar
authors.forename: N
authors.initials: N
authors.lastname: Ramanujam
authors.forename: R
authors.initials: R
authors.lastname: Richards-Kortum