Evaluation of Escherichia coli cell response to antibiotic treatment by use of Raman spectroscopy with laser tweezers.
Laser tweezers Raman spectroscopy was used to detect the cellular response of Escherichia coli cells to penicillin G-streptomycin and cefazolin. Time-dependent intensity changes of several Raman peaks at 729, 1,245, and 1,660 cm(-1) enabled untreated cells and cells treated with the different antibiotic drugs to be distinguished.
Moritz
Tobias J
TJ
Biophysics Graduate Group, University of California, Davis, Davis, California 95616, USA.
Polage
Christopher R
CR
Taylor
Douglas S
DS
Krol
Denise M
DM
Lane
Stephen M
SM
Chan
James W
JW
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001431:Bacteriological Techniques / Q000379:methods*; D002437:Cefazolin / Q000494:pharmacology; D004926:Escherichia coli / Q000737:chemistry* / Q000187:drug effects* / Q000378:metabolism; D052898:Optical Tweezers; D010400:Penicillin G / Q000494:pharmacology; D013059:Spectrum Analysis, Raman / Q000379:methods*; D013307:Streptomycin / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D002437:Cefazolin; D010400:Penicillin G; D013307:Streptomycin