Time and space resolved uptake study of silica nanoparticles by human cells.
A spatio-temporal mapping of the uptake of silica (SiO(2)) nanoparticles of different sizes by lung epithelial cells has been obtained. Based on high control of nanoparticle dispersion in cell media and cell exposure, one obtains reproducible and quantitative time-resolved data using a combination of flow cytometry, fluorescence and electron microscopies. We are thereby able to give a rather detailed account of the journey of SiO(2) nanoparticles from the early events of uptake to their final sub-cellular localization.
Shapero
Kayle
K
Centre for BioNano Interactions, School of Chemistry and Chemical Biology, UCD Conway Institute for Biomolecular and Biomedical Research, University College Dublin, Belfield, Dublin 4, Ireland.
Fenaroli
Federico
F
Lynch
Iseult
I
Cottell
David C
DC
Salvati
Anna
A
Dawson
Kenneth A
KA
D006801:Humans; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D053758:Nanoparticles; D015203:Reproducibility of Results; D012822:Silicon Dioxide / Q000378:metabolism*
D012822:Silicon Dioxide