The pH and mobile phase composition effects ochratoxin A fluorescence at liquid chromatography.
Changes in the fluorescence behavior of fungal toxic metabolite ochratoxin were studied. The influence of ratio aqueous/organic solvent, the ionic composition of solution, and pH were investigated. The chromatographic separation under conditions yielding the highest fluorescence intensity were tested and compared with results obtained under commonly used chromatographic methods. The developed method was applied on analysis of spiked and naturally contaminated cereal samples.
Dohnal
Vlastimil
V
Department of Food Technology, Faculty of Agronomy, Mendel University of Agriculture and Forestry in Brno, Zemedelska 1, 613 00 Brno, Czech Republic.
Pavlíková
Lucie
L
Kuca
Kamil
K
D000097:Acetonitriles / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D007477:Ions / Q000737:chemistry; D009793:Ochratoxins / Q000737:chemistry*; D009994:Osmolar Concentration; D013050:Spectrometry, Fluorescence / Q000379:methods*; D014908:Triticum
D000097:Acetonitriles; D007477:Ions; D009793:Ochratoxins; C025589:ochratoxin A; C032159:acetonitrile