title: Ovis aries Papillomavirus 3: a prototype of a novel genus in the family Papillomaviridae associated with ovine squamous cell carcinoma.
abstract: Papillomaviruses play an important role in human cancer development, and have been isolated from a number of animal malignancies. However, the association of papillomaviruses with tumors has been poorly investigated in sheep. In this study, a novel ovine Papillomavirus, OaPV3, was cloned from sheep squamous cell carcinoma. Unlike the already known ovine papillomaviruses, belonging to the Delta genus, OaPV3 lacks the E5 open reading frame and maintains the conserved retinoblastoma motif in the E7 gene. OaPV3 infects exclusively epithelial cells, and was found in skin of healthy sheep of geographically separated flocks located in Sardinia (Italy). This new virus is transcriptionally active in tumors and shares low homology with all the other papillomaviruses, establishing a new genus. Taken together, the co-occurrence of OaPV3 and tumors, its cell and tissue tropism, and its gene repertoire, suggests a role for this virus in development of sheep squamous cell carcinoma.
authors.affiliation: Dipartimento di Patologia e Clinica Veterinaria, Università degli Studi di Sassari, via Vienna, 2, 07100 Sassari, Italy. alberti@uniss.it
authors.forename: Alberto
authors.initials: A
authors.lastname: Alberti
authors.forename: Salvatore
authors.initials: S
authors.lastname: Pirino
authors.forename: Francesca
authors.initials: F
authors.lastname: Pintore
authors.forename: Maria Filippa
authors.initials: MF
authors.lastname: Addis
authors.forename: Bernardo
authors.initials: B
authors.lastname: Chessa
authors.forename: Carla
authors.initials: C
authors.lastname: Cacciotto
authors.forename: Tiziana
authors.initials: T
authors.lastname: Cubeddu
authors.forename: Antonio
authors.initials: A
authors.lastname: Anfossi
authors.forename: Gavino
authors.initials: G
authors.lastname: Benenati
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Coradduzza
authors.forename: Roberta
authors.initials: R
authors.lastname: Lecis
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Antuofermo
authors.forename: Laura
authors.initials: L
authors.lastname: Carcangiu
authors.forename: Marco
authors.initials: M
authors.lastname: Pittau
mesh_terms: D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000032:analysis / Q000302:isolation & purification; D004847:Epithelial Cells / Q000473:pathology / Q000821:virology; D008969:Molecular Sequence Data; D027383:Papillomaviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D030361:Papillomavirus Infections / Q000150:complications / Q000662:veterinary* / Q000821:virology; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D012756:Sheep; D012757:Sheep Diseases / Q000473:pathology / Q000821:virology*; D034561:Sheep, Domestic; D012878:Skin Neoplasms / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D013045:Species Specificity
chemical_list: D004279:DNA, Viral