title: An electrochemical supersandwich assay for sensitive and selective DNA detection in complex matrices.
abstract: In a traditional sandwich assay, a DNA target hybridizes to a single copy of the signal probe. Here we employ a modified signal probe containing a methylene blue (a redox moiety) label and a "sticky end." When a DNA target hybridizes this signal probe, the sticky end remains free to hybridize another target leading to the creation of a supersandwich structure containing multiple labels. This leads to large signal amplification upon monitoring by voltammetry.
authors.affiliation: Center for Polymers and Organic Solids, University of California, Santa Barbara, California 93106, USA.
authors.forename: Fan
authors.initials: F
authors.lastname: Xia
authors.forename: Ryan J
authors.initials: RJ
authors.lastname: White
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Zuo
authors.forename: Adriana
authors.initials: A
authors.lastname: Patterson
authors.forename: Yi
authors.initials: Y
authors.lastname: Xiao
authors.forename: Di
authors.initials: D
authors.lastname: Kang
authors.forename: Xiong
authors.initials: X
authors.lastname: Gong
authors.forename: Kevin W
authors.initials: KW
authors.lastname: Plaxco
authors.forename: Alan J
authors.initials: AJ
authors.lastname: Heeger
mesh_terms: D004247:DNA / Q000032:analysis*; D004563:Electrochemistry / Q000379:methods*; D057230:Limit of Detection; D009693:Nucleic Acid Hybridization
chemical_list: D004247:DNA