Effect of different concentrations of sevoflurane pretreatment on acute lung injury induced by endotoxin in rats.
OBJECTIVE
To investigate the effect of 3 concentrations of sevoflurane pretreatment on acute lung injury induced by endotoxin in rats.


METHODS
Thirty-six male SD rats were randomly divided into 6 groups:a control group(Group A), a sevoflurane group(Group B), a 3.6% sevoflurane pretreatment group(Group C), a 2.4% Sevoflurane pretreatment group(Group D), a 1.2% sevo sevoflurane pretreatment group(Group E), and lipopolysaccharide (Group F).The rats were killed 6 h after lipopolysaccharide (LPS) or saline injection.Histological examinations of the lung tissues were performed with light microscope. Lung wet/dry weight (W/D) ratio, myeloperoxidase (MPO) activity, and intercellular adhesion molecule-1 (ICAM-1) mRNA expression were assayed.


RESULTS
Introvenous LPS significantly aggravated lung tissue damage,increased lung W/D ratio, MPO activity, and lung ICAM-1 mRNA expression compared with the control group(P<0.05). Precondition with 2.4% sevoflurane significantly attenuated the above-mentioned changes induced by LPS (P<0.05). The 3.6% LPS (Group C) significantly attenuated lung tissue damage and decreased MPO activity,lung ICAM-1 mRNA expression compared with the Group F (P<0.05),but no significant change in lung W/D ratio was seen (P>0.05). MPO activity was significantly decreased in Group E (P<0.05), but lung W/D ratio and lung ICAM-1 mRNA expression had no significant changes (P>0.05).


CONCLUSIONS
Precondition with 2.4% sevoflurane can reduce LPS-induced lung injury in rats. Decreased expression of ICAM-1 and less accumulation of neutrophils were participated in its mechanism.
Zhao
Shuangping
S
Intensive Care Unit, Xiangya Hospital, Central South University, Changsha, China. zsp7411@yahoo.com.cn
Wu
Jiao
J
Guo
Qulian
Q
Zhang
Zhong
Z
Ye
Zhi
Z
D055371:Acute Lung Injury / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D018685:Anesthetics, Inhalation; D000818:Animals; D004305:Dose-Response Relationship, Drug; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics / Q000378:metabolism; D008070:Lipopolysaccharides; D008297:Male; D008738:Methyl Ethers / Q000494:pharmacology / Q000627:therapeutic use*; D020011:Protective Agents / Q000494:pharmacology / Q000627:therapeutic use; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D000077149:Sevoflurane
D018685:Anesthetics, Inhalation; D008070:Lipopolysaccharides; D008738:Methyl Ethers; D020011:Protective Agents; D012333:RNA, Messenger; D018799:Intercellular Adhesion Molecule-1; D000077149:Sevoflurane