Improved isoflavonoid production in Pueraria candollei hairy root cultures using elicitation.
The effect of abiotic and biotic elicitors (methyl jasmonate, chitosan, salicylic acid, Agrobacterium, and yeast extract) at various concentrations on total isoflavonoid accumulation was studied in the hairy root cultures of Pueraria candollei. All elicitors stimulated isoflavonoid production. Yeast extract (0.5 mg/ml) was the most efficient giving total isoflavonoids at 60 ± 1 mg/g dry wt, which was 4.5-fold higher than control hairy roots on day 3 of elicitation.
Udomsuk
Latiporn
L
Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand.
Jarukamjorn
Kanokwan
K
Tanaka
Hiroyuki
H
Putalun
Waraporn
W
D001709:Biotechnology / Q000379:methods*; D003470:Culture Media / Q000737:chemistry; D007529:Isoflavones / Q000378:metabolism*; D018517:Plant Roots / Q000378:metabolism*; D029908:Pueraria / Q000378:metabolism*
D003470:Culture Media; D007529:Isoflavones