An application of Ag(III) complex chemiluminescence system for the determination of enoxacin in capsule and biological fluid.
Ag(III) complex chemiluminescence (CL) system was applied for the determination of enoxacin (ENX). The CL conditions of [Ag(HIO(6))(2)](5-)-H(2)SO(4)-ENX systems without any luminescence reagent were investigated and optimized. Under the optimized conditions, the CL intensity was proportional to the concentration of ENX in the range from 6.6 × 10(-5) to 3.3 × 10(-3) g/L. The limit of detection (s/n = 3) was 2.0 × 10(-5) g/L. The recovery of ENX from the spiked pharmaceutical preparations was in the range of 82.9-108% with a relative standard deviation of 1.9-3.0%. For spiked serum and urine samples the recovery of ENX was in the range of 83.7-110% with a relative standard deviation of 1.1-2.8%. The proposed method was applied successfully to the determination of the drug in capsule, serum and urine samples.
Chen
Peiyun
P
College of Chemistry and Environmental Science, Hebei University, Key Laboratory of Analytical Science and Technology of Hebei Province, Baoding, 071002, China.
Sun
Hanwen
H
D000890:Anti-Infective Agents / Q000032:analysis*; D001826:Body Fluids / Q000737:chemistry*; D002214:Capsules / Q000737:chemistry*; D015365:Enoxacin / Q000032:analysis*; D006801:Humans; D008163:Luminescent Measurements / Q000295:instrumentation / Q000379:methods*; D018030:Silver Compounds / Q000737:chemistry*
D000890:Anti-Infective Agents; D002214:Capsules; D018030:Silver Compounds; D015365:Enoxacin