Disposable immunosensor for human cardiac troponin T based on streptavidin-microsphere modified screen-printed electrode.
Screen-printed electrodes (SPE) have been widely used in the design of disposable sensors bringing advances in the use of electrochemical immunosensors for in field-clinical analysis. In this work, streptavidin polystyrene microspheres were incorporated to the electrode surface of SPEs in order to increase the analytical response of the cardiac troponin T (cTnT), a specific biomarker for the acute myocardial infarction diagnosis. The precise calculation of the stoichiometric streptavidin-biotin ratio [1:4] allowed the increase of sensitivity and stability of the immunosensor response to the cTnT analyte. The surface of the immunosensor was characterized by scanning electron microscopy and cyclic voltammetry. It was observed that the use of streptavidin microspheres significantly increased the analytical sensitivity of the electrode in 8.5 times, showing a curve with a linear response range between 0.1 and 10 ngmL(-1) of cTnT and a detection limit of 0.2 ngmL(-1). The proposed SPE showed ease preparation and high sensitivity allowing the detection of cTnT in the range of clinical levels. The new device coupled with a portable electrochemical analyzer shows great promise for point-of-care quantitative testing of necrosis cardiac proteins.
Silva
Bárbara V M
BV
Laboratório de Pesquisa em Diagnóstico/LAPED, Pronto Socorro Cardiológico de Pernambuco/PROCAPE, Universidade de Pernambuco, Rua dos Palmares, s/n, 50100-130 Recife-PE, Brazil.
Cavalcanti
Igor T
IT
Mattos
Alessandra B
AB
Moura
Patrícia
P
Sotomayor
Maria Del Pilar T
Mdel P
Dutra
Rosa F
RF
D055910:Antibodies, Immobilized; D000911:Antibodies, Monoclonal; D015415:Biomarkers / Q000097:blood; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods / Q000706:statistics & numerical data; D055664:Electrochemical Techniques; D006801:Humans; D007118:Immunoassay; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D009203:Myocardial Infarction / Q000097:blood / Q000175:diagnosis; D015203:Reproducibility of Results; D019809:Streptavidin; D020107:Troponin T / Q000032:analysis* / Q000097:blood / Q000276:immunology
D055910:Antibodies, Immobilized; D000911:Antibodies, Monoclonal; D015415:Biomarkers; D020107:Troponin T; D019809:Streptavidin