title: A Validated RP HPLC-PAD Method for the Determination of Hederacoside C in Ivy-Thyme Cough Syrup.
abstract: A simple reversed phase high-performance liquid chromatographic (RP-HPLC) method coupled with a photodiode array detector (PAD) has been developed and validated for the analysis of hederacoside C, the marker of ivy plant, in Ivy-Thyme cough syrup. Separation of hederacoside C was achieved using a Phenomenex-Gemini C18 column isothermally at 40°C. A mobile phase system constituted of solvent A (water: acetonitrile: orthophosphoric acid (85%), 860 : 140 : 2 v/v) and solvent B (acetonitrile: orthophosphoric acid (85%), 998 : 2 v/v) was used, at gradient conditions, at a flow rate of 1.5 mL/min. Analysis was performed using UV-detection (205 nm). The method was linear over the range (0.03-0.15) mg/mL of hederacoside C (r = 0.9992). Repeatability and intermediate precision were acceptable (RSD <2%). Limits of detection (LOD) and quantitation (LOQ) were 0.011 and 0.032 mg/mL, respectively. Percentage recovery was found to lie between 99.69% and 100.90% (RSD <2%). The method was also proved to be specific (peak-purity coefficient = 0.996).
authors.affiliation: Faculty of Pharmacy, Applied Sciences University, Amman 11931, Jordan.
authors.forename: Ayman
authors.initials: A
authors.lastname: Khdair
authors.forename: Mohammad K
authors.initials: MK
authors.lastname: Mohammad
authors.forename: Khaled
authors.initials: K
authors.lastname: Tawaha
authors.forename: Eman
authors.initials: E
authors.lastname: Al-Hamarsheh
authors.forename: Hatim S
authors.initials: HS
authors.lastname: Alkhatib
authors.forename: Bashar
authors.initials: B
authors.lastname: Al-Khalidi
authors.forename: Yasser
authors.initials: Y
authors.lastname: Bustanji
authors.forename: Samer
authors.initials: S
authors.lastname: Najjar
authors.forename: Mohammad
authors.initials: M
authors.lastname: Hudaib