[Study on antioxidant effect of Herba Lycopi and screening of active fractions].
OBJECTIVE
To Study the antioxidant activity of Herba Lycopi, and screen the main active fractions.


METHODS
The different polar fractions were isolated from the ethanol extract of Herba Lycopi. The scavenging activity to DPPH and superoxide anions, reducing effect on Fe3+, and inhibiting activity of lipid peroxidation of the fractions were studied in vitro by high-throughput screening technique.


RESULTS
The ethanol extract, ethyl acetate fraction (LLE-A. E, MCCM2921) and n-buthanol fraction (LLE-A. B, MCCM2922) of Herba Lycopi showed the scavenging activity to DPPH and superoxide anions, reducing effect on Fe3+, and inhibiting activity of lipid peroxidation.


CONCLUSIONS
The ethanol extract and different polar fractions showed antioxidant capacity in vitro. That may be explain that the effect of activating blood and dissolving stasis and anti-fibrosis of Herba Lycopi in traditional application. The results may be helpful for further development of Herba Lycopi as a low-toxic antioxidant drug.
Nie
Bo
B
Key Laboratory of Chinese Internal Medicine Ministry of Education and Beijing City, Dongzhimen Hospital Affiliated to Bejing University of Chinese Medicine, Beijing 100700, China. nieboww_1977@163.com
Huang
Feng
F
Liu
Yong
Y
Du
Guanhua
G
Liang
Xinmiao
X
Xiao
Peigen
P
D000818:Animals; D000975:Antioxidants / Q000032:analysis* / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects; D031333:Lycopus / Q000737:chemistry*; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism; D010936:Plant Extracts / Q000032:analysis* / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
D000975:Antioxidants; D010936:Plant Extracts