Polymorphisms in MTHFR and MTRR genes associated with blood plasma homocysteine concentration and sperm counts.
OBJECTIVE
To investigate the relationship between MTHFR and MTRR genetic variants with respect to both blood plasma homocysteine concentration and sperm counts.


METHODS
Polymerase chain reaction followed by specific enzymatic digestion to determine the genotype of the individuals and blood plasma homocysteine quantification by high-performance liquid chromatography.


METHODS
Research laboratory.


METHODS
Two hundred sixty-eight men seeking infertility counseling and 254 partners of infertile women.


METHODS
We studied three MTHFR (c.1286A → C, c.665C → T and c.203G → A) and two MTRR (c.66A → G and c.524C → T) single-nucleotide polymorphisms and characterized sperm parameters in both oligozoospermic and normospermic men. A cohort of 522 men was examined for this study. A subgroup of 103 men was constituted for quantification of Hcy levels.


METHODS
Semen samples were collected for determinations of sperm concentration, motility, and morphology according to World Health Organization guidelines as well as for DNA isolation. Blood samples of the corresponding individuals were obtained to quantify plasma homocysteine levels.


RESULTS
We did not observe a relationship between homocysteinemia and sperm counts. The MTHFR c.665C → T variant is associated with mild hyperhomocysteinemia in blood plasma in the TT homozygous state.


CONCLUSIONS
No association was found between MTHFR/MTRR genetic variants and sperm counts. Although no association was observed with reduced sperm counts, the MTHFR 665TT genotype is associated with a significant increase in blood plasma homocysteine levels.
Montjean
Debbie
D
Advanced Technology Laboratory, La verrière, Paris, France. debbie.montjean@pasteur.fr
Benkhalifa
Moncef
M
Dessolle
Lionel
L
Cohen-Bacrie
Paul
P
Belloc
Stéphanie
S
Siffroi
Jean-Pierre
JP
Ravel
Célia
C
Bashamboo
Anu
A
McElreavey
Kenneth
K
D000328:Adult; D015331:Cohort Studies; D005260:Female; D005287:Ferredoxin-NADP Reductase / Q000235:genetics*; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006710:Homocysteine / Q000032:analysis / Q000097:blood*; D006801:Humans; D007248:Infertility, Male / Q000097:blood / Q000235:genetics* / Q000503:physiopathology; D008297:Male; D042965:Methylenetetrahydrofolate Reductase (NADPH2) / Q000235:genetics*; D009994:Osmolar Concentration; D020641:Polymorphism, Single Nucleotide / Q000502:physiology; D055101:Semen Analysis; D013076:Sperm Count
D006710:Homocysteine; C111241:methionine synthase reductase; D005287:Ferredoxin-NADP Reductase; D042965:Methylenetetrahydrofolate Reductase (NADPH2)