Electron microscopy analysis of histone acetylation and DNA strand breaks in mouse elongating spermatids using a dual labelling approach.
Chromatin remodelling steps in mammalian spermatids include posttranslational modifications of histones and DNA fragmentation. Histone H4 hyperacetylation (AcH4) establishes a chromatin state that facilitates DNA repair in somatic cells. So we sought to determine whether a similar link exists in spermatids by combining immunogold labelling with detection of DNA strand breaks, making use of gold particles of different sizes. DNA strand breaks were not detected in the vicinity of AcH4 chromatin, suggesting that this modified histone may not be involved in the aetiology of DNA fragmentation and repair in spermatids. The AcH4 reactivity, however, indicates that chromatin remodelling is distributed throughout the nucleus.
Bikond Nkoma
G
G
Department of Biochemistry, Faculty of Medicine, Université de Sherbrooke, Sherbrooke, Quebec, Canada.
Leduc
F
F
Jaouad
L
L
Boissonneault
Guylain
G
D000107:Acetylation; D000818:Animals; D002843:Chromatin / Q000378:metabolism / Q000648:ultrastructure; D042002:Chromatin Assembly and Disassembly; D053960:DNA Breaks; D004260:DNA Repair; D006657:Histones / Q000737:chemistry / Q000378:metabolism*; D007150:Immunohistochemistry / Q000379:methods*; D008297:Male; D051379:Mice; D008854:Microscopy, Electron / Q000379:methods*; D013087:Spermatids / Q000378:metabolism* / Q000648:ultrastructure*; D013737:Testis / Q000378:metabolism
D002843:Chromatin; D006657:Histones