Effects of electrodeposited poly(ethylene glycol) on biofilm adherence to titanium.
Protein-resistant coatings have been studied for inhibiting biofilm formation on implant devices. In this study, titanium (Ti) surfaces were biofunctionalized with poly(ethylene glycol) (PEG) by electrodeposition and were evaluated as biofilm substrates under an oral simulated environment. Streptococcus gordonii, an early colonizer of oral biofilms, was inoculated on Ti and PEG-electrodeposited Ti (PEG-Ti) surfaces and was analyzed quantitatively and topographically. Streptococcus mutans supplemented with sucrose, a late colonizer mainly found in dental plaque, was also used to form biofilms on the surfaces of Ti and PEG-Ti for 20 h followed by sonication as a means of detaching the biofilms. The results indicated that the attachment of S. gordonii on PEG-Ti surfaces was inhibited compared with Ti, and the S. mutans biofilm was easier to be detached from the surface of PEG-Ti than that of Ti. Moreover, the presence of PEG electrodeposited on Ti surface inhibited salivary protein adsorption. The degree of detachment of biofilms from PEG-Ti was associated with the inhibition of the salivary protein adsorption, suggesting weak basal attachment of the biofilms to the electrodeposited surfaces. Therefore, controlling protein adsorption at the initial stage of biofilm formation may be an effective strategy to protect metal surfaces from bacterial contamination not only in dental manipulations but also in orthopedic applications.
Tanaka
Yuta
Y
Department of Metals, Tokyo Medical and Dental University, Tokyo, Japan.
Matin
Khairul
K
Gyo
Mariko
M
Okada
Ayako
A
Tsutsumi
Yusuke
Y
Doi
Hisashi
H
Nomura
Naoyuki
N
Tagami
Junji
J
Hanawa
Takao
T
D000327:Adsorption / Q000187:drug effects; D001422:Bacterial Adhesion / Q000187:drug effects*; D018441:Biofilms / Q000187:drug effects*; D019149:Bioreactors / Q000382:microbiology; D004595:Electroplating / Q000379:methods*; D005936:Glucans / Q000032:analysis; D006801:Humans; D011092:Polyethylene Glycols / Q000494:pharmacology*; D012463:Saliva / Q000187:drug effects / Q000378:metabolism; D012471:Salivary Proteins and Peptides / Q000302:isolation & purification; D012995:Solubility / Q000187:drug effects; D054773:Streptococcus gordonii / Q000187:drug effects / Q000502:physiology; D013295:Streptococcus mutans / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure; D013499:Surface Properties / Q000187:drug effects; D014025:Titanium / Q000494:pharmacology*; D014867:Water / Q000737:chemistry
D005936:Glucans; D012471:Salivary Proteins and Peptides; D014867:Water; D011092:Polyethylene Glycols; D014025:Titanium