Efficient photoactivation of a Diels-Alderase ribozyme.
Here we report the first example of a photoactivatable ribozyme which catalyzes a bimolecular reaction of two small organic molecules with multiple turnover, under control of a photo-cleavable protecting group by exploiting the structural significance of a single hydrogen bond.
Nierth
Alexander
A
Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, Im Neuenheimer Feld 364, Heidelberg 69120, Germany.
Singer
Marco
M
Jäschke
Andres
A
D004789:Enzyme Activation; D031541:Fluorescence Resonance Energy Transfer; D006860:Hydrogen Bonding; D007700:Kinetics; D008958:Models, Molecular; D010777:Photochemistry; D016337:RNA, Catalytic / Q000378:metabolism*
D016337:RNA, Catalytic