title: Magnetic resonance microscopy contribution to interpret high-resolution magic angle spinning metabolomic data of human tumor tissue.
abstract: HRMAS NMR is considered a valuable technique to obtain detailed metabolic profile of unprocessed tissues. To properly interpret the HRMAS metabolomic results, detailed information of the actual state of the sample inside the rotor is needed. MRM (Magnetic Resonance Microscopy) was applied for obtaining structural and spatially localized metabolic information of the samples inside the HRMAS rotors. The tissue was observed stuck to the rotor wall under the effect of HRMAS spinning. MRM spectroscopy showed a transference of metabolites from the tissue to the medium. The sample shape and the metabolite transfer after HRMAS indicated that tissue had undergone alterations and it can not be strictly considered as intact. This must be considered when HRMAS is used for metabolic tissue characterization, and it is expected to be highly dependent on the manipulation of the sample. The localized spectroscopic information of MRM reveals the biochemical compartmentalization on tissue samples hidden in the HRMAS spectrum.
authors.affiliation: Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), ISC-III, C/Doctor Moliner 50, 46100 Burjassot, Valencia, Spain. carmen.martinez-bisbal@uv.es
authors.forename: M Carmen
authors.initials: MC
authors.lastname: Martínez-Bisbal
authors.forename: Vicent
authors.initials: V
authors.lastname: Esteve
authors.forename: Beatriz
authors.initials: B
authors.lastname: Martínez-Granados
authors.forename: Bernardo
authors.initials: B
authors.lastname: Celda
mesh_terms: D001932:Brain Neoplasms / Q000378:metabolism* / Q000473:pathology; D005910:Glioma / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D008279:Magnetic Resonance Imaging / Q000379:methods*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D055442:Metabolome; D020543:Proteome / Q000378:metabolism*; D013113:Spin Labels; D014018:Tissue Distribution; D014407:Tumor Cells, Cultured
chemical_list: D020543:Proteome; D013113:Spin Labels