Biosynthesis of plant-specific flavones and flavonols in Streptomyces venezuelae.
Recently, recombinant Streptomyces venezuelae has been established as a heterologous host for microbial production of flavanones and stilbenes, a class of plant-specific polyketides. In the present work, we expanded the applicability of the S. venezuelae system to the production of more diverse plant polyketides including flavones and flavonols. A plasmid with the synthetic codon-optimized flavone synthase I gene from Petroselium crispum was introduced to S. venezuelae DHS2001 bearing a deletion of the native pikromycin polyketide synthase gene, and the resulting strain generated flavones from exogenously fed flavanones. In addition, a recombinant S. venezuelae mutant expressing a codon-optimized flavanone 3beta-hydroxylase gene from Citrus siensis and a flavonol synthase gene from Citrus unshius also successfully produced flavonols.
Park
Sung Ryeol
SR
Department of Chemistry and Nano Science, Ewha Womans University, Seoul 120-750, Korea.
Paik
Ji Hye
JH
Ahn
Mi Sun
MS
Park
Je Won
JW
Yoon
Yeo Joon
YJ
D019661:Apiaceae / Q000201:enzymology / Q000235:genetics; D002957:Citrus / Q000201:enzymology; D047309:Flavones / Q000096:biosynthesis*; D044948:Flavonols / Q000096:biosynthesis*; D015870:Gene Expression; D017343:Genes, Plant / Q000235:genetics; D005818:Genetic Engineering; D007218:Industrial Microbiology / Q000379:methods*; D006899:Mixed Function Oxygenases / Q000235:genetics; D013302:Streptomyces / Q000235:genetics / Q000378:metabolism*
D047309:Flavones; D044948:Flavonols; D006899:Mixed Function Oxygenases; C065998:flavone synthase I; C048797:flavanone 3-dioxygenase