Separation and characterization of forced degradation products of abacavir sulphate by LC-MS/MS.
Abacavir sulphate was subjected to forced degradation under the conditions of hydrolysis (acid, alkali and neutral), oxidation, photolysis and thermal stress as prescribed by ICH. Eight degradation products were formed and their separation was accomplished on Waters XTerra C₁₈ (250 mm x 4.6 mm, 5 μm) column using 20 mM ammonium acetate:acetonitrile as a mobile phase in gradient elution mode by LC. The degradation products were characterized by LC-MS/MS and its fragmentation pathways were proposed. No previous reports were found in the literature regarding the degradation behavior of abacavir sulphate.
Rao
R Nageswara
RN
Analytical Chemistry Division, D215, Discovery Laboratory, Indian Institute of Chemical Technology, Tarnaka, Hyderabad 500607, India. rnrao55@yahoo.com
Vali
R Mastan
RM
Ramachandra
Bondigalla
B
Raju
S Satyanarayana
SS
D002853:Chromatography, Liquid; D015224:Dideoxynucleosides / Q000737:chemistry*; D017408:Guidelines as Topic / Q000592:standards; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007391:International Cooperation; D007700:Kinetics; D015394:Molecular Structure; D010084:Oxidation-Reduction; D010782:Photolysis; D015203:Reproducibility of Results; D018894:Reverse Transcriptase Inhibitors / Q000737:chemistry*; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*; D000099227:Abacavir
D015224:Dideoxynucleosides; D018894:Reverse Transcriptase Inhibitors; D000099227:Abacavir