title: [Modification of mid-point restrictor of heart cut multidimensional gas chromatography-mass spectrometry system and its application in analysis of benzo [a] pyrene in cigarette smoke].
abstract: The mid-point restrictor, known as the key spare part of multidimensional gas chromatography (MDGC), was modified and applied in the determination of benzo[a] pyrene in cigarette smoke. The graphite restrictor was replaced by a 0.25 mm i.d. fused silica open tubular and the two-hole graphite ferrule was replaced by a press-fit glass Y-splitter to improve the stability of retention time on the 1st dimensional column and eliminate the carry-over effects of graphite. The cigarette samples were prepared as follows: The mainstream cigarette smoke collected on Cambridge pad was extracted with cyclohexane, then the extractant was analyzed by heart cut MDGC-mass spectrometry (MS) directly with benzo [ a] pyrene-d12 as internal standard. The method was proved to be simple, sensitive, fast and reliable with good linearity (linear range of 1.47-29.4 microg/L, r2 of 0.9999), reproducibility (relative standard deviation (RSD) of 1.94%), recovery (90.74%-101.86%) and low quantitative and qualitative detection limits. Moreover, the amount of benzo [a] pyrene in the 2R4F Kentucky reference cigarette smoke obtained by this method was close to that reported in literature.
authors.affiliation: Technical Center, Shanghai Tobacco (Group) Corporation, Shanghai 200082, China. shijq@sh.tobacco.com.cn
authors.forename: Jiaqin
authors.initials: J
authors.lastname: Shi
authors.forename: Baizhan
authors.initials: B
authors.lastname: Liu
authors.forename: Wenyan
authors.initials: W
authors.lastname: Xie
mesh_terms: D001564:Benzo(a)pyrene / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D012906:Smoke / Q000032:analysis*; D014026:Nicotiana / Q000737:chemistry*
chemical_list: D012906:Smoke; D001564:Benzo(a)pyrene