New strategy for large-scale preparation of the extracellular domain of tumor-associated antigen HAb18G/CD147 (HAb18GED).
HAb18G/CD147, a cancer-associated biomarker, can promote tumor growth, invasion and metastasis. Here, we established a new strategy to express the extracellular domain of HAb18G/CD147 (HAb18GED) by using the FRT/Flp-In recombinase-based site-directed integration system. Two clones expressing HAb18GED were established, and 600 mg HAb18GED was obtained with more than 95% purity. Our results showed that purified HAb18GED exhibited strong activities to stimulate the secretion of pro-MMP2 and MMP-2 and promote the invasion of HCC cells. Overall, our study effectively overcame the difficulty of large-scale HAb18GED preparation and laid a solid foundation for the further studies on structure and functions of HAb18G/CD147.
Zhang
Zheng
Z
State Key Laboratory of Cancer Biology, Cell Engineering Research Centre & Department of Cell Biology, Fourth Military Medical University, Xi'an 710032, PR China.
Yang
Xiangmin
X
Yang
Hushan
H
Yu
Xiaoling
X
Li
Yu
Y
Xing
Jinliang
J
Chen
Zhinan
Z
D000818:Animals; D000951:Antigens, Neoplasm / Q000096:biosynthesis*; D051926:Basigin / Q000096:biosynthesis*; D014408:Biomarkers, Tumor / Q000096:biosynthesis; D019149:Bioreactors; D016466:CHO Cells; D006528:Carcinoma, Hepatocellular / Q000276:immunology / Q000473:pathology; D045744:Cell Line, Tumor; D006224:Cricetinae; D003412:Cricetulus; D005822:Genetic Vectors; D006801:Humans; D008113:Liver Neoplasms / Q000276:immunology / Q000473:pathology; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D016297:Mutagenesis, Site-Directed; D011994:Recombinant Proteins / Q000096:biosynthesis
D000951:Antigens, Neoplasm; C497886:BSG protein, human; D014408:Biomarkers, Tumor; D011994:Recombinant Proteins; D051926:Basigin; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2