Total chemical synthesis of human proinsulin.
A convergent synthetic strategy based on modern chemical ligation methods was used to make human proinsulin. The synthetic protein was characterized by LCMS, CD spectroscopy, and by 1D- and 2D-NMR spectroscopy. Synthetic human proinsulin had full biochemical activity in a receptor-binding assay.
Luisier
Samuel
S
Department of Chemistry, Institute for Biophysical Dynamics, The University of Chicago, Chicago, Illinois 60637, USA.
Avital-Shmilovici
Michal
M
Weiss
Michael A
MA
Kent
Stephen B H
SB
D000595:Amino Acid Sequence; D002942:Circular Dichroism; D006801:Humans; D008969:Molecular Sequence Data; D011384:Proinsulin / Q000138:chemical synthesis* / Q000737:chemistry; D013237:Stereoisomerism
D011384:Proinsulin