title: The effect of actinoquinol with hyaluronic acid in eye drops on the optical properties and oxidative damage of the rabbit cornea irradiated with UVB rays.
abstract: Irradiation of the cornea with UVB rays leads to its oxidative damage, swelling and increased light absorption. We investigated changes in the corneal optics (evaluated by changes of corneal hydration and light absorption) and microscopical disturbances of corneas irradiated with UVB rays as influenced by eye drops containing actinoquinol with hyaluronic acid. Rabbit corneas were irradiated with a daily dose of 0.5 or 1.01 J cm(-2) of UVB rays (312 nm) for 4 days. During irradiation, the eye drops were applied on the right eye and buffered saline (or hyaluronic acid) on the left eye. On day 5 the rabbits were sacrificed and the corneas examined spectrophotometrically for light absorption. The corneal thickness (hydration) was measured using a pachymeter. Corneas of some other rabbits were examined immunohistochemically. After buffered saline treatment UVB rays evoked changes in the corneal optics and induced oxidative damage of the corneas. After actinoquinol-hyaluronic acid application, these changes were diminished. Hyaluronic acid alone was less effective. In conclusion, actinoquinol-hyaluronic acid eye drops decreased changes in corneal optics and suppressed oxidative damage in the UVB-irradiated cornea. However, the effective corneal protection by these eye drops was limited to the lower UVB dose.
authors.affiliation: Laboratory of Eye Histochemistry and Pharmacology, Institute of Experimental Medicine, Center of Excellence, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
authors.forename: Čestmír
authors.initials: Č
authors.lastname: Čejka
authors.forename: Jacques
authors.initials: J
authors.lastname: Luyckx
authors.forename: Taras
authors.initials: T
authors.lastname: Ardan
authors.forename: Jan
authors.initials: J
authors.lastname: Pláteník
authors.forename: Jakub
authors.initials: J
authors.lastname: Širc
authors.forename: Jiří
authors.initials: J
authors.lastname: Michálek
authors.forename: Jitka
authors.initials: J
authors.lastname: Čejková
mesh_terms: D000818:Animals; D003315:Cornea / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects*; D003318:Corneal Opacity / Q000517:prevention & control; D006820:Hyaluronic Acid / Q000008:administration & dosage*; D009883:Ophthalmic Solutions; D018384:Oxidative Stress; D011804:Quinolines / Q000008:administration & dosage*; D011817:Rabbits; D011837:Radiation-Protective Agents / Q000008:administration & dosage; D014466:Ultraviolet Rays / Q000009:adverse effects*
chemical_list: D009883:Ophthalmic Solutions; D011804:Quinolines; D011837:Radiation-Protective Agents; C556636:actinoquinol; D006820:Hyaluronic Acid