[A possible association of fatal pneumonia with mutations of pandemic influenza A/H1N1 sw1 virus in the receptor-binding site of the HA1 subunit].
The paper gives the results of sequence analysis of 150 positive samples in real-time RT-PCR, including 47 autopsy materials from patients (including 10 pregnant women), who died from fatal pneumonia mainly in November-December 2009, in whom the lifetime etiological diagnosis had not been made and hence no early etiotropic therapy performed. 70% of the primary materials from the deceased patients were found to have pandemic influenza A(H1N1) v mutants in the lung tissue with D222G (15%), D222N (15%), D222E (2%) substitutions, as well as a mixture of mutants (38%). Nasopharyngeal lavages from 3 Chukotka deceased patients exhibited only consensus (nonmutant) D222 virus variants; there was a mixture of consensus and mutant virus variants in the trachea and a mixture of mutant ones in the lung. Preliminary data from the study of the interaction of the hemagglutinin of two strains having D222G and D222N mutations with 9 oligosaccharides imitating the variants of cell receptors for influenza A virus suggest that there is a double receptor specificity for alpha2'-3' and alpha2'-6'-sialosides with a preponderance of alpha2'-3'-specificity. Further spread of the mutants that have acquired a high virulence and preserved their capacity for the respiratory route of human infection may lead to the situation similar to that seen in the 1918-1919 pandemic. Another scenario for evolution of the virus is to preserve its receptor specificity for alpha2'-3'-sialosides and high virulence with losses of alpha2'-6' specificity and capacity for aerosol transmission, by damping the pandemic.
L'vov
D K
DK
Burtseva
E I
EI
Prilipov
A G
AG
Bogdanova
V S
VS
Shchelkanov
M Iu
MIu
Bovin
N V
NV
Samokhvalov
E I
EI
Fediakina
I T
IT
Deriabin
P G
PG
Kolobukhina
L V
LV
Shtyria
Iu A
IuA
Shevchenko
E S
ES
Malyshev
N A
NA
Merkulova
L N
LN
Bazarova
M V
MV
Maslov
A I
AI
Ishchenko
N M
NM
Iskhakova
E A
EA
Al'khovskiĭ
S V
SV
Grebennikova
T V
TV
Sadykova
G K
GK
L'vov
D N
DN
Zhuravleva
M M
MM
Iamnikova
S S
SS
Shliapnikova
O V
OV
Poglazov
A B
AB
Trushakova
S V
SV
Lavrishcheva
V V
VV
Aristova
V A
VA
Proshina
E S
ES
Vereshchagin
N N
NN
Kuz'michev
A G
AG
Iashkulov
K B
KB
Dzhambinov
S D
SD
Bushkieva
B Ts
BTs
Eliseeva
S M
SM
Bystrakov
S I
SI
Sokolova
I A
IA
Dzhaparidzhe
N I
NI
Ledenev
Iu A
IuA
Rosolovskiĭ
A P
AP
Gareev
R V
RV
Boldyreva
V V
VV
Anan'ev
V Iu
VIu
Baranov
N I
NI
Gorelikov
V N
VN
Garbuz
Iu A
IuA
Reznik
V I
VI
Ivanov
L I
LI
Zdanovskaia
N N
NN
Sergeeva
N M
NM
Podolianko
I A
IA
Elovskiĭ
O V
OV
Gromova
M A
MA
Kalaeva
E E
EE
Grigor'ev
S N
SN
Eremeeva
Iu V
IuV
Dovgal'
M V
MV
Fedelesh
I Iu
IIu
Sakharova
E A
EA
Burtnik
V I
VI
Avdoshina
L N
LN
Shapiro
N P
NP
Maslov
D V
DV
Ianovich
V A
VA
Ott
V A
VA
Lebedev
G B
GB
D001665:Binding Sites / Q000235:genetics; D004196:Disease Outbreaks; D005260:Female; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics* / Q000472:pathogenicity; D007251:Influenza, Human / Q000453:epidemiology* / Q000401:mortality / Q000821:virology*; D008168:Lung / Q000821:virology; D008297:Male; D011024:Pneumonia, Viral / Q000453:epidemiology* / Q000401:mortality / Q000821:virology*; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000453:epidemiology* / Q000401:mortality / Q000821:virology*; D021122:Protein Subunits / Q000235:genetics* / Q000378:metabolism; D011991:Receptors, Virus / Q000378:metabolism; D012426:Russia / Q000453:epidemiology; D020539:Sequence Analysis, Protein; D014774:Virulence
D019267:Hemagglutinin Glycoproteins, Influenza Virus; D021122:Protein Subunits; D011991:Receptors, Virus