title: MicroRNAs: tiny targets for engineering CHO cell phenotypes?
abstract: The ability of microRNAs to influence gene expression is now recognized as a fundamental layer of regulation within the cell. MicroRNAs have a major impact on most biological processes and have generated considerable interest as potential biomarkers as well as therapeutic or engineering targets. In this review we provide a brief overview of their biogenesis, genomic organization and mode of action, followed by a description of the methods and approaches to studying their expression. We go on to consider some of the approaches to utilizing them as tools and their potential application in the bioprocessing area, with particular emphasis on Chinese hamster ovary cell engineering.
authors.affiliation: National Institute for Cellular Biotechnology, Dublin City University, Dublin 9, Ireland. niall.barron@dcu.ie
authors.forename: Niall
authors.initials: N
authors.lastname: Barron
authors.forename: Noelia
authors.initials: N
authors.lastname: Sanchez
authors.forename: Paul
authors.initials: P
authors.lastname: Kelly
authors.forename: Martin
authors.initials: M
authors.lastname: Clynes
mesh_terms: D000818:Animals; D016466:CHO Cells; D018929:Cell Culture Techniques; D006224:Cricetinae; D003412:Cricetulus; D005786:Gene Expression Regulation; D018390:Gene Targeting / Q000379:methods*; D005818:Genetic Engineering / Q000379:methods*; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism*; D010641:Phenotype; D011994:Recombinant Proteins / Q000096:biosynthesis
chemical_list: D035683:MicroRNAs; D011994:Recombinant Proteins