[Preparation of magnetic latexes and their use for the immunodetection of microbial antigens].
The possibility of detecting antigens of plague, tularemia, and brucellosis microbes with magnetic latex (ML)-based test systems has been demonstrated. MLs were prepared from latexes (polyacroleine microspheres, 1.2-1.8 +/- 0.1 microm) by exposing the particles to a 25-35%-solution of ferrous sulfate for 0.5 h and then to a 15-25%-aqueous solution of ammonia for 0.5 h in a 100 degrees C water bath and dehydrating after each operation. The possibility of preparing magnetic latex immunosorbents (MLIS) by ligand immobilization on ML and using them in magnetic latex ELISA (ML-ELISA) for the detection of microbial antigens was demonstrated. The detection limit in ML-ELISA equaled 10(2)-10(3) microbial cells in 1 ml (cells/ml). Relative experimental error was not higher than 8%.
Kal'noĭ
S M
SM
Zharnikova
I V
IV
Zaĭtsev
A A
AA
Bondarenko
A I
AI
Borzdova
I Iu
IIu
Ostapovich
V V
VV
Kurilova
A A
AA
D000942:Antigens, Bacterial / Q000032:analysis*; D002002:Brucella / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay; D005296:Ferrous Compounds / Q000737:chemistry*; D005604:Francisella tularensis / Q000302:isolation & purification; D007164:Immunosorbents / Q000737:chemistry*; D007840:Latex / Q000737:chemistry*; D008863:Microspheres; D012680:Sensitivity and Specificity; D015010:Yersinia pestis / Q000302:isolation & purification*
D000942:Antigens, Bacterial; D005296:Ferrous Compounds; D007164:Immunosorbents; D007840:Latex; C020748:ferrous sulfate; C034236:ferrous oxide