Protein immobilization on liposomes and lipid-coated nanoparticles by protein trans-splicing.
A plethora of methods exist to link proteins to surfaces in order to generate functionalized materials. However, general tools that lead to functional immobilization of recombinantly expressed proteins on membranes such as liposomes or lipid-coated nanoparticles are rare. Here we present an approach that takes advantage of a double-palmitoylated peptide that mediates stable membrane anchoring in combination with protein trans-splicing for efficient immobilization of recombinant proteins fused to split intein segments. Two different DnaE split inteins from Synechocystis and Nostoc punctiforme are tested and compared to immobilization via direct native chemical ligation using a protein thioester. Protein trans-splicing proceeds at low protein concentrations and leads to functionalized vesicles and membrane-coated silica nanoparticles.
Chu
Nam Ky
NK
Center for Integrated Protein Science Munich and Technische Universität München, Fakultät Chemie, Garching, Germany.
Olschewski
Diana
D
Seidel
Ralf
R
Winklhofer
Konstanze F
KF
Tatzelt
Jörg
J
Engelhard
Martin
M
Becker
Christian F W
CF
D055767:Immobilized Proteins / Q000737:chemistry / Q000378:metabolism; D047668:Inteins / Q000235:genetics; D008055:Lipids / Q000737:chemistry*; D008081:Liposomes / Q000378:metabolism*; D008958:Models, Molecular; D053758:Nanoparticles / Q000737:chemistry*; D046937:Nostoc / Q000235:genetics; D019154:Protein Splicing; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D046939:Synechocystis / Q000235:genetics; D020040:Trans-Splicing
D055767:Immobilized Proteins; D008055:Lipids; D008081:Liposomes; D011993:Recombinant Fusion Proteins