Cognitive impairment and changes of neuronal plasticity in rats of chronic cerebral hypoperfusion associated with cerebral arteriovenous malformations.
A new rat model associated with cerebral arteriovenous malformations (AVMs) was developed to study the effect of chronic cerebral hypoperfusion on cognitive function and neuronal plasticity in rats. Aged-matched animals comprised a control group. Three months after surgery, Morris water waze test was performed to evaluate the cognitive function in rats. Neuronal plasticity was assessed by measuring the protein expression of MAP-2, GAP-43 and synaptophysin in the hippocampal regions of rats with immunohistochemistry and western blotting. The average time of escape latency was significantly longer in the model rats than that in the control rats, and both the time spent in the platform quadrant and the frequency of original platform crossing during space probe trials were less than those in the control animals. The expression levels of MAP-2 and synaptophysin protein in hippocampal areas in the model rats were less than those in the control rats. However there was no difference on the GAP-43 expression between the two groups. These data suggest that chronic cerebral hypoperfusion associated with AVMs could lead to cognitive impairment in rats, which may be partially explained by reduced expression of MAP-2 and synaptophysin at the protein level in the hippocampal area.
Hai
Jian
J
Department of Neurosurgery, Tongji Hospital, Tongji University, Shanghai 200065, China. haijiandoc@yahoo.com.cn
Su
Sao-Hua
SH
Lin
Qi
Q
Zhang
Lin
L
Wan
Jue-Feng
JF
Li
Hui
H
Chen
Yu-Ying
YY
Lu
Yang
Y
D000818:Animals; D002545:Brain Ischemia / Q000150:complications*; D002560:Cerebrovascular Circulation; D002908:Chronic Disease; D003072:Cognition Disorders / Q000209:etiology*; D004195:Disease Models, Animal; D007839:Functional Laterality; D019922:GAP-43 Protein / Q000378:metabolism; D005786:Gene Expression Regulation / Q000502:physiology; D006624:Hippocampus / Q000378:metabolism; D002538:Intracranial Arteriovenous Malformations / Q000150:complications*; D008026:Ligation / Q000379:methods; D018782:Maze Learning / Q000502:physiology; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D009473:Neuronal Plasticity / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D016708:Synaptophysin / Q000378:metabolism; D013997:Time Factors
D019922:GAP-43 Protein; C489787:MAP2 protein, rat; D008869:Microtubule-Associated Proteins; D016708:Synaptophysin