[Comparative protein analysis of K562 cell apoptosis induced by 6-gingerol].
OBJECTIVE
To establish two-dimensional electrophoresis (2-DE) pattern for proteome analysis on K562 cells before and after treatment with 6-gingerol, and mass-spectrometry was applied to identify and analyze the differentially expressed proteins.


METHODS
K562 cells were treated with 6-gingerol. Cell proliferation was analyzed by CCK-8 assay. Total protein of K562 cells was extracted by 2D-DIGE and then imaged by SDS gel scanning. The differentially expressed proteins were identified using Imagine Master 2D Platinum 6.0 software and functionally classified by MALDI and MALDI-TOF MS.


RESULTS
6-gingerol could significantly inhibit K562 cells proliferation and the efficacy was concentration and dose-dependent. After being treated for 24, 48, 72 h, IC50 was 22.86, 15.75, 11.18 microg/mL, respectively. 42 differentially expressed proteins were identified, including 19 up-regulated expressed proteins and 10 down-regulated expressed proteins.


CONCLUSIONS
Proteomic technique can be used to screen multiple proteins associate with the anti-leukemia effect of 6-gingerol, involving some important proteins related to oxidative stress, cell cycle regulation, apoptosis signal transduction, biosynthesis and glycometabolism.
Zeng
Hui-lan
HL
Department of Hematology, the First Affiliated Hospital, Jinan University, Guangzhou 510632, China.
Han
Xin-ai
XA
Gu
Chen
C
Huang
Xue-song
XS
Gu
Jian-quan
JQ
Zhong
Qi
Q
Ming
Wei-jie
WJ
Cai
Xian-ni
XN
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002396:Catechols / Q000008:administration & dosage / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D005233:Fatty Alcohols / Q000008:administration & dosage / Q000494:pharmacology*; D020939:Zingiber officinale / Q000737:chemistry; D006801:Humans; D020014:K562 Cells; D025941:Protein Interaction Mapping; D011506:Proteins / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism*; D020543:Proteome / Q000032:analysis / Q000378:metabolism; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D057825:Two-Dimensional Difference Gel Electrophoresis
D000972:Antineoplastic Agents, Phytogenic; D002396:Catechols; D005233:Fatty Alcohols; D011506:Proteins; D020543:Proteome; C007845:gingerol