The complete mitochondrial genome of Radix balthica (Pulmonata, Basommatophora), obtained by low coverage shot gun next generation sequencing.
A 454-FLX low-coverage sequencing approach was used to assemble the mitochondrial genome of Radix balthica. The mtDNA sequence is 13,993 nt long and contains 37 genes (13 protein coding genes, two rRNAs and 22 tRNAs). Four genes, the 12S RNA and seven tRNAs are transcribed in reverse order. The sequence is AT rich (71.3%), similar to other basommatophoran species. Comparison with the most closely related mt genomes available (Biomphalaria glabrata and Biomphalaria tenagophila) revealed identical gene orders except for five tRNAs. Next generation sequencing proved to be a fast and easy method for sequencing an entire mitochondrial genome.
Feldmeyer
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B
Biodiversity and Climate Research Center (BiK-F), Siesmayerstr. 70A, 60323 Frankfurt am Main, Germany. barbara.feldmeyer@senckenberg.de
Hoffmeier
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K
Pfenninger
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D000818:Animals; D055028:Comparative Genomic Hybridization; D004272:DNA, Mitochondrial / Q000235:genetics; D023061:Gene Order; D054629:Genome, Mitochondrial; D012343:RNA, Transfer / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000379:methods*; D012908:Snails / Q000145:classification / Q000235:genetics*
D004272:DNA, Mitochondrial; D012343:RNA, Transfer