Tracking clathrin coated pits with a multiple hypothesis based method.
Cellular processes are crucial for cells to survive and function properly. To study their underlying mechanisms quantitatively with fluorescent live cell microscopy, it is necessary to track a large number of particles involved in these processes. In this paper, we present a method to automatically track particles, called clathrin coated pits (CCPs), which are formed in clathrin mediated endocytosis (CME). The tracking method is developed based on a MAP framework, and it consists of particle detection and trajectory estimation. To detect particles in 2D images and take account of Poisson noise, a Gaussian mixture model is fitted to image data, for which initial parameters are provided by a combination of image filtering and histogram based thresholding methods. A multiple hypothesis based algorithm is developed to estimate the trajectories based on detection data. To use the current knowledge about CCPs, their properties of motion and intensity are considered in our models. The tracking method is evaluated on synthetic data and real data, and experimental results show that it has high accuracy and is in good agreement with manual tracking.
Liang
Liang
L
Yale University, New Haven, CT 06520, USA. liang.liang@yale.edu
Shen
Hongying
H
De Camilli
Pietro
P
Duncan
James S
JS
D000465:Algorithms; D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D022163:Clathrin-Coated Vesicles / Q000648:ultrastructure*; D007089:Image Enhancement / Q000379:methods; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008853:Microscopy; D010363:Pattern Recognition, Automated / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013382:Subtraction Technique