title: Suppressive effects of intravenous immunoglobulin (IVIG) on human umbilical cord blood immune cells.
abstract: We investigated in vitro immunomodulatory effects of intravenous immunoglobulin (IVIG) on cord blood mononuclear cells (CBMNC), macrophages, dendritic cells and CD3(+) T cells were isolated from umbilical venous blood. Cell proliferation used (3)H-TdR incorporation, culture supernatants were assayed for cytokines using ELISA, and surface marker expressions were determined by flow cytometry. IVIG suppressed CBMNCs and CD3(+) T-cells proliferation, secretions of IL-10, INF-γ and TGF-β(1), but not IL-4, and PHA-induced expressions of surface molecules (CD25, CD45RA and CD45RO), with more pronounced effects for CBMNCs. IVIG decreased cord blood (CB) macrophage phagocytosis and CD14, HLA-DR and CD86 expressions. IVIG increased CD14 expression and decreased MCH II expression for differentiation-stage CB dendritic cells (DCs) and increased CD14 expression and decreased CD80 and CD83 expressions of mature DCs, suggesting that IVIG intervention inhibited DC differentiation and maturation. In addition to T cells, IVIG immunomodulatory effects on CBMNCs involve a variety of cells and molecules. CB macrophages and CBMNC-DCs are targets of IVIG.
authors.affiliation: Department of Pediatrics, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Jihong
authors.initials: J
authors.lastname: Qian
authors.forename: Jianxing
authors.initials: J
authors.lastname: Zhu
authors.forename: Meihong
authors.initials: M
authors.lastname: Wang
authors.forename: Shengmei
authors.initials: S
authors.lastname: Wu
authors.forename: Tongxin
authors.initials: T
authors.lastname: Chen
mesh_terms: D015703:Antigens, CD; D018122:B7-1 Antigen; D017252:CD3 Complex; D002454:Cell Differentiation / Q000276:immunology; D002478:Cells, Cultured; D016207:Cytokines / Q000276:immunology / Q000378:metabolism; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism*; D005312:Fetal Blood / Q000166:cytology / Q000276:immunology; D005434:Flow Cytometry; D006801:Humans; D007136:Immunoglobulins; D016756:Immunoglobulins, Intravenous / Q000276:immunology* / Q000494:pharmacology*; D007231:Infant, Newborn; D017493:Leukocyte Common Antigens; D018950:Lipopolysaccharide Receptors; D008264:Macrophages / Q000276:immunology / Q000378:metabolism*; D008562:Membrane Glycoproteins; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D000097802:CD83 Antigen
chemical_list: D015703:Antigens, CD; D018122:B7-1 Antigen; D017252:CD3 Complex; D016207:Cytokines; D007136:Immunoglobulins; D016756:Immunoglobulins, Intravenous; D017493:Leukocyte Common Antigens; D018950:Lipopolysaccharide Receptors; D008562:Membrane Glycoproteins; D000097802:CD83 Antigen; C516769:PTPRC protein, human