title: Molecular characterization, distribution, and dynamics of hepatitis C virus genotypes in blood donors in Colombia.
abstract: Hepatitis C virus (HCV) is a frequent cause of acute and chronic hepatitis and a leading cause for cirrhosis of the liver and hepatocellular carcinoma. HCV is classified in six major genotypes and more than 70 subtypes. In Colombian blood banks, serum samples were tested for anti-HCV antibodies using a third-generation ELISA. The aim of this study was to characterize the viral sequences in plasma of 184 volunteer blood donors who attended the "Banco Nacional de Sangre de la Cruz Roja Colombiana," Bogotá, Colombia. Three different HCV genomic regions were amplified by nested PCR. The first of these was a segment of 180 bp of the 5'UTR region to confirm the previous diagnosis by ELISA. From those that were positive to the 5'UTR region, two further segments were amplified for genotyping and subtyping by phylogenetic analysis: a segment of 380 bp from the NS5B region; and a segment of 391 bp from the E1 region. The distribution of HCV subtypes was: 1b (82.8%), 1a (5.7%), 2a (5.7%), 2b (2.8%), and 3a (2.8%). By applying Bayesian Markov chain Monte Carlo simulation, it was estimated that HCV-1b was introduced into Bogotá around 1950. Also, this subtype spread at an exponential rate between about 1970 to about 1990, after which transmission of HCV was reduced by anti-HCV testing of this population. Among Colombian blood donors, HCV genotype 1b is the most frequent genotype, especially in large urban conglomerates such as Bogotá, as is the case in other South American countries.
authors.affiliation: Laboratory of Gastroenterology and Hepatology, Department of Gastroenterology, School of Medicine, São Paulo Institute of Tropical Medicine, University of São Paulo, São Paulo, Brazil. monica.viviana@usp.br
authors.forename: Mónica Viviana Alvarado
authors.initials: MV
authors.lastname: Mora
authors.forename: Camila Malta
authors.initials: CM
authors.lastname: Romano
authors.forename: Michele Soares
authors.initials: MS
authors.lastname: Gomes-Gouvêa
authors.forename: Maria Fernanda
authors.initials: MF
authors.lastname: Gutiérrez
authors.forename: Flair José
authors.initials: FJ
authors.lastname: Carrilho
authors.forename: João Renato Rebello
authors.initials: JR
authors.lastname: Pinho
mesh_terms: D000328:Adult; D001499:Bayes Theorem; D001782:Blood Donors; D003105:Colombia / Q000453:epidemiology; D005260:Female; D005838:Genotype; D016174:Hepacivirus / Q000145:classification* / Q000235:genetics* / Q000276:immunology; D006526:Hepatitis C / Q000175:diagnosis / Q000453:epidemiology* / Q000276:immunology / Q000821:virology*; D018937:Hepatitis C Antibodies / Q000097:blood; D006801:Humans; D008297:Male; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D015995:Prevalence; D017422:Sequence Analysis, DNA
chemical_list: D018937:Hepatitis C Antibodies