title: The electrochemiluminescence of ruthenium complex/tripropylamine systems at DNA-modified gold electrodes.
abstract: The electrochemiluminescence (ECL) behavior of ruthenium complex/tripropylamine (TPA) systems at DNA-modified gold electrode was studied to understand the possible mechanism and to develop new detection platforms. DNA strand, especially double-stranded DNA (ds-DNA), can preconcentrate TPA and acts as the acceptor of the protons released from TPAH(+), therefore the improved ECL emission and the low potential ECL were observed. The intercalation of Ru(phen)(3)(2+) into ds-DNA was confirmed to be a sensitive and label-free DNA-related detection platform. The above results were validated by the analysis of lysozyme using anti-lysozyme aptamer-modified electrode. This work opens a new field by the use of DNA-modified electrode to develop novel sensing platforms, such as low potential ECL biosensors and Ru(phen)(3)(2+) intercalation-based ECL biosensors.
authors.affiliation: Research Center for Analytical Sciences, College of Chemistry, Nankai University, Tianjin 300071, PR China.
authors.forename: Dong-Yuan
authors.initials: DY
authors.lastname: Liu
authors.forename: You-Ying
authors.initials: YY
authors.lastname: Xin
authors.forename: Xi-Wen
authors.initials: XW
authors.lastname: He
authors.forename: Xue-Bo
authors.initials: XB
authors.lastname: Yin
mesh_terms: D017076:Computer-Aided Design; D004247:DNA / Q000737:chemistry*; D004566:Electrodes; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006046:Gold / Q000737:chemistry*; D008163:Luminescent Measurements / Q000295:instrumentation*; D011437:Propylamines / Q000737:chemistry*; D012428:Ruthenium / Q000032:analysis* / Q000737:chemistry*
chemical_list: D011437:Propylamines; C096226:tripropylamine; D006046:Gold; D012428:Ruthenium; D004247:DNA