title: Detection of DNA fragmentation and meiotic segregation in human with isolated teratozoospermia.
abstract: OBJECTIVE
  To evaluate levels of DNA fragmentation and chromosomal abnormalities in ejaculated sperm of males with isolated teratozoospermia and to determine if specific sperm morphological types occur simultaneously with these nuclear defects.
  
  
  METHODS
  Sperm obtained from isolated teratozoospermic men (n = 70) and fertile men (n = 30) were analysed using fluorescence in situ hybridization and TUNEL assay.
  
  
  RESULTS
  Teratozoospermic men, compared to fertile men, showed significantly higher rates of sex chromosomes disomy, and diploidy. Significant correlations were found between amorphous head, microcephalic head, short tail, and sex chromosomes disomy. Level of sperm DNA fragmentation was significantly higher in teratozoospermic men than in controls and positively correlated to the incidence of macrocephalic heads, amorphous heads, and short flagella.
  
  
  CONCLUSIONS
  Patients with isolated teratozoospermia have increased levels of DNA fragmentation and chromosomal aneuploidy. Some specific morphological abnormalities were shown to be predictive of chromosomal abnormalities and DNA alteration.
authors.affiliation: Department of Cytogenetic and Reproductive biology, Farhat Hached, University Teaching Hospital, Sousse, Tunisia, brahemsonia@yahoo.fr
authors.forename: Sonia
authors.initials: S
authors.lastname: Brahem
authors.forename: Meriem
authors.initials: M
authors.lastname: Mehdi
authors.forename: Hatem
authors.initials: H
authors.lastname: Elghezal
authors.forename: Ali
authors.initials: A
authors.lastname: Saad
mesh_terms: D000328:Adult; D000782:Aneuploidy; D053938:DNA Fragmentation; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D007248:Infertility, Male / Q000235:genetics*; D008297:Male; D008540:Meiosis; D011446:Prospective Studies; D013094:Spermatozoa / Q000002:abnormalities*