Droplet-based microfluidic platforms for single T cell secretion analysis of IL-10 cytokine.
Here we present a microfluidic method for the analysis of single cell secretions. The method co-encapsulates cells with microspheres conjugated with capture antibodies and detection fluorescence-labeled antibodies. The secreted substance captured on the microsphere surface and detected via detection antibodies generating a localized fluorescent signal on a microsphere surface. Using this method, CD4+CD25+ regulatory T cells were encapsulated and assayed to detect IL-10 secreting cell in population.
Konry
Tania
T
Center for Engineering in Medicine and Surgical Services, Massachusetts General Hospital, Harvard Medical School, 51 Blossom St., Boston, MA, USA. konry.tania@gmail.com
Dominguez-Villar
Margarita
M
Baecher-Allan
Clare
C
Hafler
David A
DA
Yarmush
Martin L
ML
D002469:Cell Separation / Q000295:instrumentation*; D002478:Cells, Cultured; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D016753:Interleukin-10 / Q000378:metabolism*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D013601:T-Lymphocytes / Q000378:metabolism*
D016753:Interleukin-10