Stability-indicating spectrophotometric methods for determination of tazarotene in the presence of its alkaline degradation product by derivative spectrophotometric techniques.
The stability of tazarotene (TZ) was investigated and two stability-indicating methods-namely, first derivative and a derivative ratio spectrophotometric method-were used to determine tazarotene in the presence of its alkaline degradation product (HD) using methanol as a solvent. A linear relationship was obtained in the range 1-10 µg ml⁻¹ for both methods. By applying the proposed methods, it was possible to determine tazarotene in its pure powdered from with accuracy 99.35 ± 1.410 (n = 10) for the first derivative method and 99.45 ± 1.053 (n = 10) for the derivative ratio method. First derivative and derivative ratio methods were used for the analysis of laboratory-prepared mixtures containing different ratios of tazarotene and its degradation product and they were valid in the presence of up to 70% and 80% degradation product, respectively. The proposed methods were validated and found to be suitable as stability-indicating assay methods for tazarotene in pharmaceutical formulations.
Badawy
Amr M
AM
Cairo University, Department of Analytical Chemistry, Cairo-Egypt.
El-Alim
Abd El-Aziz B Abd
Ael-A
Saad
Ahmed S
AS
D002138:Calibration; D004355:Drug Stability; D006868:Hydrolysis; D006878:Hydroxides / Q000737:chemistry*; D013058:Mass Spectrometry; D015394:Molecular Structure; D009539:Nicotinic Acids / Q000032:analysis* / Q000737:chemistry; D017680:Potassium Compounds / Q000737:chemistry*; D015203:Reproducibility of Results; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D012176:Retinoids
D006878:Hydroxides; D009539:Nicotinic Acids; D017680:Potassium Compounds; C086827:tazarotene; D012176:Retinoids; C029943:potassium hydroxide