Cryptotomography: reconstructing 3D Fourier intensities from randomly oriented single-shot diffraction patterns.
We reconstructed the 3D Fourier intensity distribution of monodisperse prolate nanoparticles using single-shot 2D coherent diffraction patterns collected at DESY's FLASH facility when a bright, coherent, ultrafast x-ray pulse intercepted individual particles of random, unmeasured orientations. This first experimental demonstration of cryptotomography extended the expansion-maximization-compression framework to accommodate unmeasured fluctuations in photon fluence and loss of data due to saturation or background scatter. This work is an important step towards realizing single-shot diffraction imaging of single biomolecules.
Loh
N D
ND
Laboratory of Atomic and Solid State Physics, Cornell High Energy Synchrotron Source (CHESS), Cornell University, Ithaca, New York 14853-2501, USA.
Bogan
M J
MJ
Elser
V
V
Barty
A
A
Boutet
S
S
Bajt
S
S
Hajdu
J
J
Ekeberg
T
T
Maia
F R N C
FR
Schulz
J
J
Seibert
M M
MM
Iwan
B
B
Timneanu
N
N
Marchesini
S
S
Schlichting
I
I
Shoeman
R L
RL
Lomb
L
L
Frank
M
M
Liang
M
M
Chapman
H N
HN
D005240:Feasibility Studies; D005290:Ferric Compounds / Q000737:chemistry; D005583:Fourier Analysis; D021621:Imaging, Three-Dimensional / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry; D012542:Scattering, Radiation; D014054:Tomography / Q000379:methods*
D005290:Ferric Compounds; C000499:ferric oxide