[Induction of human dental pulp stem cells of premolars into osteoblast-like cells by mineralizing culture medium].
OBJECTIVE
To induce human dental pulp stem cells(HDPSCs) of premolars into osteoblast-like cells by mineralizing culture medium.


METHODS
HDPSCs of premolars were induced by mineralizing culture medium for 28 days.The activity of ALP was examined by enzyme histochemical staining on the 7th and 28th day. The ability of mineralization of HDPSCs was detected by Alizarin-red staining on the 14th, 21st and 28th day. The gene expression of ALP, DSPP, BSP, OCN on day 0, 3, 5, 7, 14, 21 and 28 was evaluated by RT-PCR. The protein expression of BSP, OCN was analyzed by Western blotting and immunocytochemistry.


RESULTS
ALP was expressed and mineralized nodules were observed after induction of HDPSCs. ALP mRNA was detected since the 3rd day, while no expression of DSPP mRNA. The gene of BSP, OCN was expressed from the 5th day and exhibited increment with time. The expression trend of BSP protein was consistent with BSP mRNA. OCN stained positive since the 5th day.


CONCLUSIONS
HDPSCs of premolars could be induced to differentiate into osteoblast-like cells,which suggests that HDPSCs of premolars may be a potential source of cells used for bone-tissue engineering in the future. Supported by National Natural Science Foundation of China (Grant No.30772452).
Zhang
Xiao-yan
XY
Department of Orthodontics, Peking University School and Hospital of Stomatology. Beijing 100081, China.
Li
Xiao-tong
XT
Zeng
Xiang-long
XL
D001641:Bicuspid; D002454:Cell Differentiation; D003782:Dental Pulp; D006801:Humans; D010006:Osteoblasts; D013234:Stem Cells