Yeast elicitor-induced stomatal closure and peroxidase-mediated ROS production in Arabidopsis.
Yeast elicitor (YEL) induces stomatal closure. We investigated reactive oxygen species (ROS) production, nitric oxide (NO) production and [Ca(2+)](cyt) oscillations to clarify YEL signaling in Arabidopsis guard cells. YEL induced ROS accumulation in guard cells. A peroxidase inhibitor [salicylhydroxamic acid (SHAM)] inhibited the stomatal closure and the ROS accumulation, but neither the atrbohD atrbohF mutation nor an NADPH oxidase inhibitor [diphenylene iodonium chloride (DPI)] had any effect. An NO scavenger [2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO)] inhibited the YEL-induced stomatal closure and SHAM abolished NO production. YEL-elicited [Ca(2+)](cyt) oscillations were inhibited by SHAM but not by the atrbohD atrbohF mutation. These results indicate that YEL induces stomatal closure accompanied by ROS production mediated by peroxidases and NO production.
Khokon
Md Atiqur Rahman
MA
Graduate School of Natural Science and Technology, Division of Bioscience, Okayama University, 1-1-1 Tsushima-Naka, Okayama, 700-8530 Japan.
Hossain
Mohammad Anowar
MA
Munemasa
Shintaro
S
Uraji
Misugi
M
Nakamura
Yoshimasa
Y
Mori
Izumi C
IC
Murata
Yoshiyuki
Y
D017360:Arabidopsis / Q000201:enzymology / Q000378:metabolism* / Q000502:physiology; D002118:Calcium / Q000378:metabolism; D010544:Peroxidases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D015398:Signal Transduction; D015003:Yeasts / Q000378:metabolism*
D017382:Reactive Oxygen Species; D010544:Peroxidases; D002118:Calcium