title: Emergence of oseltamivir-resistant pandemic (H1N1) 2009 virus within 48 hours.
abstract: An oseltamivir-resistant influenza A pandemic (H1N1) 2009 virus evolved and emerged from zero to 52% of detectable virus within 48 hours of a patient's exposure to oseltamivir. Phylogenetic analysis and data gathered by pyrosequencing and cloning directly on clinical samples suggest that the mutant emerged de novo.
authors.affiliation: Agency for Science, Research and Technology, Singapore.
authors.forename: Masafumi
authors.initials: M
authors.lastname: Inoue
authors.forename: Timothy
authors.initials: T
authors.lastname: Barkham
authors.forename: Yee Sin
authors.initials: YS
authors.lastname: Leo
authors.forename: Kwai Peng
authors.initials: KP
authors.lastname: Chan
authors.forename: Angela
authors.initials: A
authors.lastname: Chow
authors.forename: Christopher W
authors.initials: CW
authors.lastname: Wong
authors.forename: Raphael
authors.initials: R
authors.lastname: Tze Chuen Lee
authors.forename: Sebastian
authors.initials: S
authors.lastname: Maurer-Stroh
authors.forename: Raymond
authors.initials: R
authors.lastname: Lin
authors.forename: Cui
authors.initials: C
authors.lastname: Lin
mesh_terms: D000328:Adult; D000998:Antiviral Agents / Q000494:pharmacology*; D024882:Drug Resistance, Viral / Q000235:genetics*; D019143:Evolution, Molecular; D005260:Female; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity; D007251:Influenza, Human / Q000188:drug therapy* / Q000453:epidemiology / Q000821:virology; D009154:Mutation; D053139:Oseltamivir / Q000494:pharmacology*; D058873:Pandemics; D010802:Phylogeny; D017422:Sequence Analysis, DNA / Q000379:methods; D013997:Time Factors
chemical_list: D000998:Antiviral Agents; D053139:Oseltamivir