DLC-1 as a modulator of proliferation, apoptosis and migration in Burkitt's lymphoma cells.
Deleted in liver cancer-1(DLC-1) gene expression is frequently down-regulated or deleted in many types of human cancer. To evaluate whether DLC-1 could be a therapeutic target for non-Hodgkin lymphoma (NHL), we examined the expressions of DLC-1 in Burkitt's lymphoma (BL) cell lines and tested the effects of DLC-1 on cellular growth and migration in BL cells. DLC-1 expression was not detectable in two human BL cell lines, Raji and Daudi, by reverse transcription-PCR. The transfer of DLC-1 into Raji and Daudi cell lines caused a significant inhibition in cell proliferation. This inhibitory effect on cell proliferation in BL cell lines was accompanied by induction of apoptosis. Furthermore, restoration of DLC-1 expression in BL cells had a significant inhibitory effect on migration. Our findings suggest that DLC-1 may play an important role in lymphoma by acting as a bona fide new tumor suppressor gene.
Feng
Minhua
M
Ningbo College of Health Science, Ningbo 315000, China.
Huang
Bo
B
Du
Zunguo
Z
Xu
Xiaoping
X
Chen
Zi
Z
D017209:Apoptosis; D002051:Burkitt Lymphoma / Q000235:genetics / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D003094:Collagen / Q000378:metabolism; D004338:Drug Combinations; D020690:GTPase-Activating Proteins / Q000235:genetics / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007797:Laminin / Q000378:metabolism; D011509:Proteoglycans / Q000378:metabolism; D014162:Transfection; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism*; D051028:bcl-2-Associated X Protein / Q000378:metabolism
C112352:DLC1 protein, human; D004338:Drug Combinations; D020690:GTPase-Activating Proteins; D007797:Laminin; D011509:Proteoglycans; D025521:Tumor Suppressor Proteins; D051028:bcl-2-Associated X Protein; C059485:matrigel; D003094:Collagen