The absence of the luxS gene increases swimming motility and flagella synthesis in Escherichia coli K12.
Despite the significant role of S-ribosylhomocysteinase (LuxS) in the activated methyl cycle pathway and quorum sensing, the connectivity between luxS and other cellular functions remains incomplete. Herein, we show that luxS deletion significantly increases swimming motility and flagella synthesis in Escherichia coli K12 using motility, transcriptome, and scanning electron microscopy assays. Further, based on the transcriptome and network component analyses, and known regulatory relations, we propose a conceptual genetic regulatory network underlying the increased flagella synthesis in response to luxS deletion.
Ling
Hua
H
School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore 637459, Singapore.
Kang
Aram
A
Tan
Mui Hua
MH
Qi
Xiaobao
X
Chang
Matthew Wook
MW
D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology*; D013437:Carbon-Sulfur Lyases / Q000235:genetics / Q000502:physiology*; D048168:Escherichia coli K12 / Q000235:genetics / Q000502:physiology*; D005407:Flagella / Q000235:genetics / Q000502:physiology*; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial
D001426:Bacterial Proteins; D013437:Carbon-Sulfur Lyases; C117753:LuxS protein, Bacteria