Photocatalytic formation of I-I bonds using DNA which enables detection of single nucleotide polymorphisms.
By decreasing the HOMO energy gap between the base-pairs to increase the charge conductivity of DNA, the positive charge photochemically generated in DNA can be made to migrate along the π-way of DNA over long distances to form a long-lived charge-separated state. By fine-tuning the kinetics of the charge-transfer reactions, we designed a functionalized DNA system in which absorbed photon energy is converted into chemical energy to form I-I covalent bonds, where more than 100 I(2) molecules were produced per functionalized DNA. Utilizing the fact that charge-transfer kinetics through DNA is sensitive to the presence of a single mismatch that causes the perturbation of the π-stacks, single nucleotide polymorphisms (SNPs) in genomic sequences were detected by measuring the photon energy conversion efficiency in DNA by a conventional starch iodine method.
Kawai
Kiyohiko
K
The Institute of Scientific and Industrial Research (SANKEN), Osaka University, Mihogaoka 8-1, Ibaraki, Osaka 567-0047, Japan. kiyohiko@sanken.osaka-u.ac.jp
Kodera
Haruka
H
Majima
Tetsuro
T
D001483:Base Sequence; D002384:Catalysis; D004247:DNA / Q000737:chemistry*; D017931:DNA Primers; D007700:Kinetics; D010777:Photochemistry; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide
D017931:DNA Primers; D004247:DNA