An extracellular loop of the mannose phosphotransferase system component IIC is responsible for specific targeting by class IIa bacteriocins.
Class IIa bacteriocins target a phylogenetically defined subgroup of mannose-phosphotransferase systems (man-PTS) on sensitive cells. By the use of man-PTS genes of the sensitive Listeria monocytogenes (mpt) and the nonsensitive Lactococcus lactis (ptn) species to rationally design a series of man-PTS chimeras and site-directed mutations, we identified an extracellular loop of the membrane-located protein MptC that was responsible for specific target recognition by the class IIa bacteriocins.
Kjos
Morten
M
Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, As, Norway.
Salehian
Zhian
Z
Nes
Ingolf F
IF
Diep
Dzung B
DB
D000595:Amino Acid Sequence; D000898:Antibiosis; D001426:Bacterial Proteins / Q000037:antagonists & inhibitors*; D001430:Bacteriocins / Q000378:metabolism / Q000494:pharmacology*; D013294:Lactococcus lactis / Q000187:drug effects / Q000201:enzymology*; D008089:Listeria monocytogenes / Q000187:drug effects / Q000201:enzymology*; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D050505:Mutant Proteins / Q000235:genetics / Q000378:metabolism; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000037:antagonists & inhibitors*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D011995:Recombination, Genetic; D016415:Sequence Alignment
D001426:Bacterial Proteins; D001430:Bacteriocins; D050505:Mutant Proteins; D011994:Recombinant Proteins; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; C037992:phosphoenolpyruvate-mannose phosphotransferase