Porous-CLEAs of papain: application to enzymatic hydrolysis of macromolecules.
Porous cross-linked enzyme aggregates (p-CLEAs) were prepared by adding starch as a pore-making agent, which facilitates CLEAs in cases where the substrates of enzyme are macromolecules. This novel strategy for preparation of p-CLEAs involves co-precipitation, cross-linking and removal of starch by α-amylase. The resulting papain p-CLEAs were characterized by scanning electron microscope (SEM) images, showing a porous structure. The 95.9% and 90.4% increased catalytic efficiencies of p-CLEAs than conventional CLEAs on bovine serum albumin (BSA) and ovalbumin verified the feasibility of this protocol.
Wang
Mengfan
M
State Key Laboratory of Chemical Engineering, Chemical Engineering Research Center, School of Chemical Engineering and Technology, Tianjin University, Tianjin, PR China.
Jia
Chenxi
C
Qi
Wei
W
Yu
Qingxin
Q
Peng
Xin
X
Su
Rongxin
R
He
Zhimin
Z
D004789:Enzyme Activation; D004800:Enzymes, Immobilized / Q000737:chemistry; D006868:Hydrolysis; D046911:Macromolecular Substances / Q000737:chemistry*; D010206:Papain / Q000737:chemistry*; D016062:Porosity; D013213:Starch / Q000737:chemistry*
D004800:Enzymes, Immobilized; D046911:Macromolecular Substances; D013213:Starch; D010206:Papain