Novel apramycin resistance gene apmA in bovine and porcine methicillin-resistant Staphylococcus aureus ST398 isolates.
A novel apramycin resistance gene, apmA, was detected on the ca.-40-kb resistance plasmid pAFS11 from bovine methicillin-resistant Staphylococcus aureus (MRSA) of sequence type 398 (ST398). The apmA gene coded for a protein of 274 amino acids that was related only distantly to acetyltransferases involved in chloramphenicol or streptogramin A resistance. NsiI deletion of apmA resulted in a 16- to 32-fold decrease in the apramycin MICs. An apmA-specific PCR identified this gene in one additional bovine and four porcine MRSA ST398 isolates.
Fessler
Andrea T
AT
Institute of Farm Animal Genetics, Friedrich-Loeffler-Institut, Neustadt-Mariensee, Germany.
Kadlec
Kristina
K
Schwarz
Stefan
S
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics*; D002417:Cattle; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D009328:Nebramycin / Q000031:analogs & derivatives* / Q000494:pharmacology; D016133:Polymerase Chain Reaction; D013552:Swine
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D009328:Nebramycin; C011666:apramycin