title: Gene expression patterns in the outer mantle epithelial cells associated with pearl sac formation.
abstract: For pearl culture, nucleus and mantle grafts are implanted into the gonad of the host oyster. The epithelial cells of the implanted mantle graft elongate and surround the nucleus, and a pearl sac is formed. Shell matrix proteins secreted by the pearl sac play an important role in pearl formation. We studied the gene expression patterns of six shell matrix proteins (msi60, n16, nacrein, msi31, prismalin-14, and aspein) in the epithelial cells associated with pearl sac formation. There were differences in the expression patterns of the six genes in the epithelial cells, and the relative expression levels for msi60 and aspein differed between the mantle graft and pearl sac (48 days after implantation). Therefore, the gene expression patterns of the epithelial cells were genetically undetermined, and changed between before and after pearl sac formation. The gene expression patterns of the epithelial cells of the pearl sac may be regulated by the host oysters.
authors.affiliation: Faculty of Bioresources, Mie University, 1577 Kurimamachiya, Tsu, Mie, 514-0501, Japan. nariaki23@hotmail.com
authors.forename: Nariaki
authors.initials: N
authors.lastname: Inoue
authors.forename: Ryo
authors.initials: R
authors.lastname: Ishibashi
authors.forename: Takashi
authors.initials: T
authors.lastname: Ishikawa
authors.forename: Takashi
authors.initials: T
authors.lastname: Atsumi
authors.forename: Hideo
authors.initials: H
authors.lastname: Aoki
authors.forename: Akira
authors.initials: A
authors.lastname: Komaru
mesh_terms: D060105:Animal Shells / Q000166:cytology*; D000818:Animals; D002256:Carbonic Anhydrases / Q000378:metabolism*; D018076:DNA, Complementary / Q000096:biosynthesis; D004847:Epithelial Cells / Q000378:metabolism*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000502:physiology*; D006066:Gonads / Q000378:metabolism*; D049911:Pinctada / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D018076:DNA, Complementary; C101387:nacrein; D002256:Carbonic Anhydrases