The effects of polymer phase ratio and feeding strategy on solid-liquid TPPBs for the production of L-phenylacetylcarbinol from benzaldehyde using Candida utilis.
To increase the bioproduction of L-phenylacetylcarbinol (PAC), a precursor molecule in the synthesis of the decongestants ephedrine and pseudoephedrine and which suffers from substrate, product, and by-product inhibition, by ensuring that the delivery of the substrate, benzaldehyde, is maintained within a strict concentration window. Beads of the commercial polymer, Hytrel G3548L, can act as a sequestering phase to reduce inhibitory effects to cells of Candida utilis while creating a reservoir for high concentrations of products. In this work we varied the polymer phase volume ratio (from 3 to 15%), and modified the benzaldehyde feeding strategy to further improve on system performance, resulting in greater than 100% increase in the PAC productivity relative to a single phase control, as well as robust operation of the two-phase bioreactor with minimal operator intervention.
Khan
Tanya R
TR
Department of Chemical Engineering, Queen's University, Kingston, ON, K7L 3N6, Canada.
Daugulis
Andrew J
AJ
D000096:Acetone / Q000031:analogs & derivatives* / Q000378:metabolism; D001547:Benzaldehydes / Q000378:metabolism*; D001709:Biotechnology / Q000379:methods*; D002175:Candida / Q000378:metabolism*; D008863:Microspheres; D011091:Polyesters / Q000378:metabolism*
D001547:Benzaldehydes; C059046:Hytrel; D011091:Polyesters; D000096:Acetone; C035293:1-hydroxy-1-phenyl-2-propanone; C032175:benzaldehyde