title: [Isolation, identification, and degrading effect of a pyrene-degrading strain SE12].
abstract: Using pyrene as the sole carbon and energy source, and by the method of plate sublimation, a strain SE12 was isolated from a contaminated soil near Woniushan Coking Plant in Xuzhou, China. According to the morphological, biochemical, and 16S rDNA analyses, this strain was identified as Mycobacterium sp., with 98% of homology to the rapid-growth nonpathogenic strain M. austroafricanum ATCC 33464. The optimum pH and temperature for the degradation of pyrene by SE12 were pH 9 and 30 degrees C. When the soil samples were added with 100 and 200 mg x kg(-1) of pyrene and inoculated with 10(7) CFU x g(-1) of SE12, the degradation rates of pyrene reached to 97% and 99%, respectively after 28 days incubation at 30 degrees C. By using primer-pairs nidAF/nidAR and nidBF/nidBR for amplification of ring-hydroxylating dioxygenase genes, it was shown that SE12 had the fragments of encoded large and small subunits of dioxygenase genes. Sequence analysis showed that these fragments were highly homologous to the known dioxygenase genes from pyrene-degrading Mycobacteria sp.
authors.affiliation: Jiangsu Key Laboratory for Solid Organic Waste Utilization, Nanjing Agricultural University, Nanjing 210095, China. huijingzi448@163.com
authors.forename: Qiao-qiao
authors.initials: QQ
authors.lastname: Zhang
authors.forename: Ye-jun
authors.initials: YJ
authors.lastname: Zhao
authors.forename: Chao-guang
authors.initials: CG
authors.lastname: Yang
authors.forename: Fen-wu
authors.initials: FW
authors.lastname: Liu
authors.forename: Jian
authors.initials: J
authors.lastname: He
authors.forename: Biao
authors.initials: B
authors.lastname: Shen
authors.forename: Wei
authors.initials: W
authors.lastname: Ran
mesh_terms: D001673:Biodegradation, Environmental; D009161:Mycobacterium / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D011721:Pyrenes / Q000378:metabolism*; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*
chemical_list: D011721:Pyrenes; D012989:Soil Pollutants; C030984:pyrene