title: Anti-leishmanial effects of purified compounds from aerial parts of Baccharis uncinella C. DC. (Asteraceae).
abstract: Species of Baccharis exhibit antibiotic, antiseptic, wound-healing, and anti-protozoal properties, and have been used in the traditional medicine of South America for the treatment of several diseases. In the present work, the fractionation of EtOH extract from aerial parts of Baccharis uncinella indicated that the isolated compounds caffeic acid and pectolinaringenin showed inhibitory activity against Leishmania (L.) amazonensis and Leishmania (V.) braziliensis promastigotes, respectively. Moreover, amastigote forms of both species were highly sensible to the fraction composed by oleanolic + ursolic acids and pectolinaringenin. Caffeic acid also inhibited amastigote forms of L. (L.) amazonensis, but this effect was weak in L. (V.) braziliensis amastigotes. The treatment of infected macrophages with these compounds did not alter the levels of nitrates, indicating a direct effect of the compounds on amastigote stages. The results presented herein suggest that the active components from B. uncinella can be important to the design of new drugs against American tegumentar leishmaniases.
authors.affiliation: Laboratório de Patologia de Moléstias Infecciosas, Departamento de Patologia, Faculdade de Medicina, Universidade de São Paulo, 01246-903 São Paulo, Brazil. felipepassero@usp.br
authors.forename: Luiz Felipe D
authors.initials: LF
authors.lastname: Passero
authors.forename: Alexis
authors.initials: A
authors.lastname: Bonfim-Melo
authors.forename: Carlos Eduardo P
authors.initials: CE
authors.lastname: Corbett
authors.forename: Márcia D
authors.initials: MD
authors.lastname: Laurenti
authors.forename: Marcos H
authors.initials: MH
authors.lastname: Toyama
authors.forename: Daniela O
authors.initials: DO
authors.lastname: de Toyama
authors.forename: Paulete
authors.initials: P
authors.lastname: Romoff
authors.forename: Oriana A
authors.initials: OA
authors.lastname: Fávero
authors.forename: Simone S
authors.initials: SS
authors.lastname: dos Grecco
authors.forename: Cynthia A
authors.initials: CA
authors.lastname: Zalewsky
authors.forename: João Henrique G
authors.initials: JH
authors.lastname: Lago
mesh_terms: D000818:Animals; D031175:Baccharis / Q000737:chemistry*; D002109:Caffeic Acids / Q000494:pharmacology; D002460:Cell Line; D002867:Chromones / Q000494:pharmacology; D007892:Leishmania braziliensis / Q000187:drug effects* / Q000254:growth & development; D007894:Leishmania mexicana / Q000187:drug effects* / Q000254:growth & development; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism / Q000469:parasitology; D009682:Magnetic Resonance Spectroscopy; D008519:Medicine, Traditional; D051379:Mice; D009566:Nitrates / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D009828:Oleanolic Acid / Q000494:pharmacology; D021261:Parasitic Sensitivity Tests; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry; D014315:Triterpenes / Q000494:pharmacology; D000097245:Ursolic Acid
chemical_list: D002109:Caffeic Acids; D002867:Chromones; D009566:Nitrates; D009569:Nitric Oxide; D009828:Oleanolic Acid; D010936:Plant Extracts; D014315:Triterpenes; D000097245:Ursolic Acid; C528671:pectolinarigenin; C040048:caffeic acid