title: In vitro evaluation of cytotoxicity of silver-containing borate bioactive glass.
abstract: The cytotoxicity of silver-containing borate bioactive glass was evaluated in vitro from the response of osteoblastic and fibroblastic cells in media containing the dissolution products of the glass. Glass frits containing 0-2 weight percent (wt %) Ag were prepared by a conventional melting and quenching process. The amount of Ag dissolved from the glass into a simulated body fluid (SBF), measured using atomic emission spectroscopy, increased rapidly within the first 48 h, but slowed considerably at longer times. Structural and microchemical analysis showed that the formation of a hydroxyapatite-like layer on the glass surface within 14 days of immersion in the SBF. The response of MC3T3-E1 and L929 cells to the dissolution products of the glass was evaluated using SEM observation of cell morphology, and assays of MTT hydrolysis, lactate dehydrogenase release, and alkaline phosphatase activity after incubation for up to 48 h. Cytotoxic effects were found for the borate glass containing 2 wt % Ag, but not for 0.75 and 1 wt % Ag. This borate glass containing up to ∼1 wt % Ag could provide a coating material for bacterial inhibition and enhanced bioactivity of orthopaedic implant materials such as titanium.
authors.affiliation: Department of Orthopaedic Surgery, Shanghai Sixth People's Hospital, Jiaotong University, Shanghai 200233, China.
authors.forename: Shi-Hua
authors.initials: SH
authors.lastname: Luo
authors.forename: Wei
authors.initials: W
authors.lastname: Xiao
authors.forename: Xiao-Juan
authors.initials: XJ
authors.lastname: Wei
authors.forename: Wei-Tao
authors.initials: WT
authors.lastname: Jia
authors.forename: Chang-Qing
authors.initials: CQ
authors.lastname: Zhang
authors.forename: Wen-Hai
authors.initials: WH
authors.lastname: Huang
authors.forename: Dong-Xu
authors.initials: DX
authors.lastname: Jin
authors.forename: Mohamed N
authors.initials: MN
authors.lastname: Rahaman
authors.forename: Delbert E
authors.initials: DE
authors.lastname: Day
mesh_terms: D016475:3T3 Cells; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001888:Boric Acids; D002478:Cells, Cultured; D005898:Glass; D066298:In Vitro Techniques; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D051379:Mice; D008855:Microscopy, Electron, Scanning; D012834:Silver
chemical_list: D001888:Boric Acids; D012834:Silver; D007770:L-Lactate Dehydrogenase; D000469:Alkaline Phosphatase; C032688:boric acid