Claudin-6, 7, or 9 overexpression in the human gastric adenocarcinoma cell line AGS increases its invasiveness, migration, and proliferation rate.
Altered claudin expression is related to metastatic potential, poor prognosis, or tumor recurrence. We analyzed if the overexpression of claudin-6, claudin-7, or claudin-9 in AGS cells altered cell motility, invasiveness, or proliferation rate. Claudin-7, claudin-9, and claudin-6 enhanced their invasive potential by 3.4-fold, 1.6-fold, and 2.0-fold, respectively. Claudin-6 and claudin-9 enhanced cell migration, while the proliferation rate of claudin-6-, claudin-7-, and claudin-9-transfected cells increased by 12.7%, 9.0%, and 13.3%, respectively. Claudin-7 and claudin-9 overexpression increased claudin-1 and zonula occludens-1 levels. In summary, individual increased expression of claudin-6, claudin-7, or claudin-9 is sufficient to enhance tumorigenic properties of a gastric adenocarcinoma cell line.
Zavala-Zendejas
Veronica Elizabeth
VE
Depto. Bioquímica, Facultad de Medicina, UNAM, México.
Torres-Martinez
Ana Cristina
AC
Salas-Morales
Berenice
B
Fortoul
Teresa Imelda
TI
Montaño
Luis Felipe
LF
Rendon-Huerta
Erika Patricia
EP
D000199:Actins / Q000378:metabolism; D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D062445:Claudin-1; D057167:Claudins; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D010750:Phosphoproteins / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D022502:Stress Fibers / Q000378:metabolism; D013997:Time Factors; D014162:Transfection; D015854:Up-Regulation; D062826:Zonula Occludens-1 Protein
D000199:Actins; C568108:CLDN1 protein, human; C491875:CLDN2 protein, human; C473255:CLDN7 protein, human; C524757:CLDN9 protein, human; D062445:Claudin-1; D057167:Claudins; D008565:Membrane Proteins; D010750:Phosphoproteins; D012333:RNA, Messenger; C569042:TJP1 protein, human; D062826:Zonula Occludens-1 Protein; C122101:claudin 6