A novel method for procuring a large quantity of mature murine eosinophils in vivo.
Eosinophils are rare hematopietic cells that normally constitute only 1~3% of peripheral blood leukocytes. It would be of help for the purpose of research to obtain a large quantity of eosinophils. In this study, we wanted to develop a novel strategy to induce massive expansion of murine eosinophils in vivo, based on the observation showing that treatment of IL-33 induces eosinophilia in mice. We generated an EL-4 lymphoma cell line (herein named EL-4-IL-33) that was engineered to secrete an active form of IL-33. We found that Siglec-F(+) granulocyte numbers increased by 1850-fold in the peritoneal cavity 10 days after inoculation with 1×10⁷ EL-4-IL-33 cells. This number corresponds to 74-fold increase, as compared with the number of Siglec-F(+) granulocytes in mice that received wild-type EL-4 cells. Siglec-F(+) granulocytes expanded by IL-33 had the circular nucleus and expressed eosinophil-specific genes. They also showed some functional characteristics of eosinophils in that they had the ability to respond to IL-5 for survival and eotaxin-1 for chemoattaxis and to produce bioactive eosinophil peroxidase, suggesting that these cells are genuine eosinophils. Our results indicate that sustained secretion of IL-33 by lymphoma cells in the peritoneal cavity is highly effective in increasing peritoneal eosinophil numbers. Therefore, our simple method to obtain eosinphils on a large scale might be of value for eosinophil studies.
Kim
Wonyoung
W
School of Biological Sciences, University of Ulsan, Ulsan, Republic of Korea.
Kim
Juyang
J
Shin
Changshik
C
Kim
Hyunju
H
Doh
Younkyung
Y
Cho
Hong R
HR
Kwon
Byungsuk
B
D000818:Animals; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects / Q000276:immunology; D002470:Cell Survival / Q000187:drug effects / Q000276:immunology; D054413:Chemokine CCL11 / Q000494:pharmacology; D004802:Eosinophilia / Q000139:chemically induced / Q000276:immunology; D004804:Eosinophils / Q000166:cytology* / Q000276:immunology; D000067596:Interleukin-33; D015848:Interleukin-5 / Q000276:immunology / Q000494:pharmacology; D007378:Interleukins / Q000009:adverse effects / Q000096:biosynthesis / Q000276:immunology* / Q000494:pharmacology; D051379:Mice; D010529:Peritoneal Cavity / Q000166:cytology*
D054413:Chemokine CCL11; C506535:Il33 protein, mouse; D000067596:Interleukin-33; D015848:Interleukin-5; D007378:Interleukins