title: Immunohistochemical analysis of protein expression during Aedes aegypti development.
abstract: Blood-feeding mosquitoes, including the dengue and yellow fever vector Aedes aegypti, transmit many of the world's deadliest diseases. Such diseases have resurged in developing countries and pose clear threats for epidemic outbreaks in developed countries. Recent mosquito genome projects have stimulated interest in the potential for arthropod-borne disease control by genetic manipulation of vector insects, and gene products that regulate development are of particular interest. This protocol for immunohistochemical analysis of protein expression can be used to analyze expression of developmental proteins of interest in Ae. aegypti embryos, larvae, and pupae, which will be critical for the development of markers for particular developing tissues.
authors.affiliation: Department of Medical and Molecular Genetics, Indiana University School of Medicine, South Bend, IN 46617, USA.
authors.forename: Anthony
authors.initials: A
authors.lastname: Clemons
authors.forename: Ellen
authors.initials: E
authors.lastname: Flannery
authors.forename: Kristopher
authors.initials: K
authors.lastname: Kast
authors.forename: David
authors.initials: D
authors.lastname: Severson
authors.forename: Molly
authors.initials: M
authors.lastname: Duman-Scheel
mesh_terms: D000330:Aedes / Q000254:growth & development* / Q000378:metabolism*; D000818:Animals; D018507:Gene Expression Regulation, Developmental; D007150:Immunohistochemistry; D007303:Insect Vectors / Q000254:growth & development* / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism*
chemical_list: D011506:Proteins