An amphiphilic fluorescent probe for the visualization of histamine in living cells.
A novel fluorescent probe composed of two moieties, Nile Red and an iminodiacetic acid-Ni(2+) complex, for the detection of histamine in living cells is described. The probe was successfully applied to visualizing histamine in RAW264 cells, representing the first demonstration of the imaging of histamine itself in living cells.
Seto
Daisuke
D
Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744, Moto-oka, Nishi-ku, Fukuoka 819-0395, Japan.
Soh
Nobuaki
N
Nakano
Koji
K
Imato
Toshihiko
T
D000818:Animals; D002460:Cell Line; D005456:Fluorescent Dyes / Q000378:metabolism*; D006632:Histamine / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism; D051379:Mice; D008856:Microscopy, Fluorescence
D005456:Fluorescent Dyes; D006632:Histamine