The heterogeneous nuclear ribonucleoprotein K is important for Herpes simplex virus-1 propagation.
The heterogeneous nuclear ribonucleoprotein (hnRNP) K is an evolutionarily conserved protein with roles in signal transduction and gene expression. An impact of hnRNP K on the life cycle of a broad range of viral pathogens was reported while functional data for herpesviruses were lacking. In this study we show that hnRNP K is important for Herpes simplex virus 1 egress. In absence of hnRNP K, viral entry, gene expression, viral DNA replication, and maturation of nuclear particles appear normal whereas release of infectious virions to the extracellular space was significantly affected. Our results indicate that hnRNP K has an impact on a late step of herpesviral propagation making it a potential antiviral target.
Schmidt
Tina
T
Universität des Saarlandes, Abteilung für Transplantations- und Infektionsimmunologie, Homburg/Saar, Germany.
Striebinger
Hannah
H
Haas
Jürgen
J
Bailer
Susanne M
SM
D000818:Animals; D015153:Blotting, Western; D002522:Chlorocebus aethiops; D004279:DNA, Viral / Q000235:genetics; D006367:HeLa Cells; D018259:Herpesvirus 1, Human / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D034641:Heterogeneous-Nuclear Ribonucleoprotein K / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008856:Microscopy, Fluorescence; D016133:Polymerase Chain Reaction; D034622:RNA Interference / Q000502:physiology*; D014709:Vero Cells; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism; D014779:Virus Replication / Q000235:genetics / Q000502:physiology*
D004279:DNA, Viral; D034641:Heterogeneous-Nuclear Ribonucleoprotein K; D014764:Viral Proteins