Identification of perilipin-2 as a lipid droplet protein regulated in oocytes during maturation.
Lipid droplet proteins regulate the storage and utilisation of intracellular lipids. Evidence is emerging that oocyte lipid utilisation impacts embryo development, but lipid droplet proteins have not been studied in oocytes. The aim of the present study was to characterise the size and localisation of lipid droplets in mouse oocytes during the periovulatory period and to identify lipid droplet proteins as potential biomarkers of oocyte lipid content. Oocyte lipid droplets, visualised using a novel method of staining cumulus-oocyte complexes (COCs) with BODIPY 493/503, were small and diffuse in oocytes of preovulatory COCs, but larger and more centrally located after maturation in response to ovulatory human chorionic gonadotrophin (hCG) in vivo, or FSH + epidermal growth factor in vitro. Lipid droplet proteins perilipin, perilipin-2, cell death-inducing DNA fragmentation factor 45-like effector (CIDE)-A and CIDE-B were detected in the mouse ovary by immunohistochemistry, but only perilipin-2 was associated with lipid droplets in the oocyte. In COCs, perilipin-2 mRNA and protein increased in response to ovulatory hCG. IVM failed to induce perilipin-2 mRNA, yet oocyte lipid content was increased in this context, indicating that perilipin-2 is not necessarily reflective of relative oocyte lipid content. Thus, perilipin-2 is a lipid droplet protein in oocytes and its induction in the COC concurrent with dynamic reorganisation of lipid droplets suggests marked changes in lipid utilisation during oocyte maturation.
Yang
Xing
X
The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong Province, 510080, China.
Dunning
Kylie R
KR
Wu
Linda L-Y
LL
Hickey
Theresa E
TE
Norman
Robert J
RJ
Russell
Darryl L
DL
Liang
Xiaoyan
X
Robker
Rebecca L
RL
D000818:Animals; D001896:Boron Compounds; D002352:Carrier Proteins; D002478:Cells, Cultured; D006063:Chorionic Gonadotropin / Q000008:administration & dosage; D054885:Cumulus Cells / Q000187:drug effects / Q000378:metabolism*; D022083:Cytoplasmic Structures; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005456:Fluorescent Dyes; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D018507:Gene Expression Regulation, Developmental; D007150:Immunohistochemistry; D050356:Lipid Metabolism / Q000187:drug effects; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D018613:Microscopy, Confocal; D009865:Oocytes / Q000187:drug effects / Q000378:metabolism*; D009866:Oogenesis / Q000187:drug effects; D010060:Ovulation / Q000187:drug effects; D010062:Ovulation Induction; D000070778:Perilipin-1; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
C527198:4,4-difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D002352:Carrier Proteins; D006063:Chorionic Gonadotropin; D005456:Fluorescent Dyes; D000070778:Perilipin-1; D010750:Phosphoproteins; D012333:RNA, Messenger; D004815:Epidermal Growth Factor; D005640:Follicle Stimulating Hormone