[Determination of prim-O-glucosylcimifugin and 5-O-methylvisammisoide in Saposhnikovia divaricata and HPLC fingerprint analysis].
OBJECTIVE
To develop HPLC methods for the determination of prim-O-glucosylcimifugin and 5-O-methylvisammisoide in Saposhnicovia divaricata and of HPLC fingerprint to compare the wild and culture varieties.


METHODS
Conditions of determination: Shimadzu C18 column (4.6 mm x 150 mm, 5 microm), methanol-water (40:60) as mobile phase with the flow rate of 1 mL x min(-1). The detection wavelength was 254 nm. Conditions of HPLC fingerprint: MG II C18 column (4.6 mm x 250 mm, 5 microm), the mobile phase was acetonitrile-water with the flow rate of 1 mL x min(-1), using linear gradient elution, the column temperature was 30 degrees C.


RESULTS
The average recovery of prim-O-glucosylcimifugin was 99.6% (RSD 0.72%, n=6). The average recovery of 5-O-methylvisammisoide was 102.6% (RSD 0.88%, n=6). The contents of prim-o-glucosylcimifugin in wild and culture varieties were (4.96 +/- 2.59) and (3.61 +/- 1.82) mg x g(-1) respectively. The contents of 5-O-methylvisammisoide were (3.91 +/- 2.09) and (4.37 +/- 2.02) mg x g(-1) respectively. The compositions of S. divaricata were effective separated under the conditions of HPLC fingerprint.


CONCLUSIONS
The HPLC determination method of prim-O-glucosylcimifugin and 5-O-methylvisammisoide is convenient and accurate. The HPLC fingerprint analysis method could be a basis for quality control and classification evaluate of S. divaricata.
Ma
Lijie
L
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China. nmmalj@hotmail.com
Yang
Bin
B
Feng
Xuefeng
X
Yin
Xiaojie
X
Li
Hua
H
Ge
Xiaoguang
X
Zhu
Lige
L
Cao
Jun
J
D019661:Apiaceae / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000302:isolation & purification; D009005:Monosaccharides / Q000032:analysis* / Q000302:isolation & purification; D011786:Quality Control; D014966:Xanthenes / Q000032:analysis* / Q000302:isolation & purification
C552901:5-O-methylvisammisoide; D004365:Drugs, Chinese Herbal; D009005:Monosaccharides; D014966:Xanthenes; C498945:prim-O-glucosylcimifugin