Nitric oxide-supplemented resuscitation improves early gastrointestinal blood flow in rats subjected to hemorrhagic shock without late consequences.
BACKGROUND
we have shown that hemorrhage/resuscitation altered gastrointestinal blood flow (GI-BF) and that gastric perfusion did not recover after resuscitation. This study aimed to determine the effect of nitric oxide (NO) supplemented resuscitation on the mean arterial blood pressure (MAP), GI-BF, and outcome after hemorrhagic shock.


METHODS
rats were subjected to hemorrhage and resuscitation with/without the NO-donor S-nitroso human serum albumin (S-NO-HSA). GI-BF was determined using colored microspheres.


RESULTS
NO supplementation significantly decreased MAP at the end of resuscitation. At the same time point, the GI-BF has significantly increased in the stomach, duodenum, and colon. Two hours after treatment discontinuation, there was no difference in either MAP or GI-BF between NO-supplemented and control groups. The survival times indicated that S-NO-HSA treatment was noninferior compared with control.


CONCLUSIONS
NO-supplemented resuscitation improves the GI-BF during the early stage of resuscitation without a negative impact on short-/long-term survival despite a transient MAP decrease.
Sobhian
Babak
B
Ludwig Boltzmann Institute of Experimental and Clinical Traumatology and Research Center of the Allgemeine Unfallversicherungsanstalt, Donaueschingenstr 13, A-1200, Vienna, Austria.
Jafarmadar
Mohammad
M
Redl
Heinz
H
Bahrami
Soheyl
S
D000818:Animals; D001794:Blood Pressure / Q000187:drug effects*; D005440:Fluid Therapy; D041981:Gastrointestinal Tract / Q000098:blood supply* / Q000187:drug effects; D008297:Male; D009603:Nitroso Compounds / Q000008:administration & dosage*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012710:Serum Albumin, Bovine / Q000008:administration & dosage*; D012771:Shock, Hemorrhagic / Q000150:complications / Q000401:mortality / Q000628:therapy*; D014665:Vasodilator Agents / Q000008:administration & dosage*
D009603:Nitroso Compounds; C091665:S-nitrosoalbumin; D014665:Vasodilator Agents; D012710:Serum Albumin, Bovine