title: Prevalence and diversity of carbapenemases among imipenem-nonsusceptible Acinetobacter isolates in Korea: emergence of a novel OXA-182.
abstract: Increase in multidrug-resistant Acinetobacter poses a serious problem in Korea. In this study, 190 imipenem (IPM)-nonsusceptible (NS) Acinetobacter isolates from 12 Korean hospitals in 2007 were used to determine species, prevalence, and antimicrobial susceptibility of OXA carbapenemase- and metallo-β-lactamase (MBL)-producing isolates. bla(OXA)-₂₃-like and ISAba1-asssociated bla(OXA)-₅₁-like genes were detected in 80% and 12% of 178 IPM-NS Acinetobacter baumannii isolates, respectively. A novel bla(OXA)-₁₈₂ was detected in 12 IPM-NS A. baumannii isolates. Twelve out of 14 MBL-producing isolates were non-baumanniiAcinetobacter. A. baumannii isolates with OXA carbapenemase were more often resistant to aminoglycosides, ciprofloxacin, and tigecycline than non-baumannii Acinetobacter isolates with MBL. Identical pulsed- field gel electrophoresis patterns were observed in 89% of A. baumannii isolates with bla(OXA)-₂₃-like gene. In conclusion, extremely rapid increase of IPM-NS A. baumannii in previous Korean studies was mainly due to clonal spread of OXA-23-producing A. baumannii isolates. A novel OXA-182 emerged in Korea.
authors.affiliation: Korean Institute of Tuberculosis, Seoul 137-900, South Korea.
authors.forename: Chang-Ki
authors.initials: CK
authors.lastname: Kim
authors.forename: Yangsoon
authors.initials: Y
authors.lastname: Lee
authors.forename: Hyukmin
authors.initials: H
authors.lastname: Lee
authors.forename: Gun-Jo
authors.initials: GJ
authors.lastname: Woo
authors.forename: Wonkeun
authors.initials: W
authors.lastname: Song
authors.forename: Mi-Na
authors.initials: MN
authors.lastname: Kim
authors.forename: Wee-Gyo
authors.initials: WG
authors.lastname: Lee
authors.forename: Seok Hoon
authors.initials: SH
authors.lastname: Jeong
authors.forename: Kyungwon
authors.initials: K
authors.lastname: Lee
authors.forename: Yunsop
authors.initials: Y
authors.lastname: Chong
mesh_terms: D000150:Acinetobacter / Q000145:classification / Q000187:drug effects / Q000201:enzymology* / Q000302:isolation & purification; D000151:Acinetobacter Infections / Q000453:epidemiology* / Q000382:microbiology; D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D014644:Genetic Variation; D006801:Humans; D015378:Imipenem / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D015995:Prevalence; D056910:Republic of Korea / Q000453:epidemiology; D013045:Species Specificity; D001618:beta-Lactamases / Q000096:biosynthesis / Q000737:chemistry / Q000145:classification / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D015378:Imipenem; D001618:beta-Lactamases; C063912:carbapenemase