[Features of interaction of monoclonal antibody B2 with polycyclicaromatic hydrocarbons and peptide-mimotope of benzo[a]pyrene].
To define of possible mechanism of monoclonal antibody B2 interaction with haptens and peptide-mimotope of benzo[a]pyrene Fab-fragment structure was modeled. Antibody active centre structure was defined using docking with a number of different polyclic aromatic hydrocarbons. It was shown that active center of monoclonal antibody B2 composed of two binding pockets. Correlation was revealed between experimental and calculated data on relative free energies of monoclonal antibody B2 binding with different ligands. We found that synthetic peptide-mimotope of benzo[a]pyrene weakly competeswith benzo[a]pyrene conjugate in monoclonal antibody B2 binding. Immunization of mice with peptide-mimotope conjugate did not revealed antibodies to benzo[a]pyrene. To define structural features of peptide-mimotope of benzo[a]pyrene preliminary model of peptide-mimotope in the frame of pIII protein was calculated. It was shown that tryptophan located inside of peptide can be exhibited on a surface and accessible to antibody. Obtained modeling results could be applied for following optimizations ofbenzo[a]pyrene peptide-mimotope and active center of monoclonal antibody B2.
Glushko
A N
AN
Apal'ko
S V
SV
Bakulina
A Iu
AIu
Matveeva
V A
VA
Khrapov
E A
EA
Kostianko
M V
MV
Sil'nikov
V N
VN
Filipenko
M L
ML
D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000276:immunology; D001564:Benzo(a)pyrene / Q000737:chemistry*; D001666:Binding Sites, Antibody / Q000276:immunology; D006241:Haptens / Q000737:chemistry* / Q000276:immunology; D007140:Immunoglobulin Fab Fragments / Q000737:chemistry* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008958:Models, Molecular; D010455:Peptides / Q000737:chemistry* / Q000276:immunology
D000911:Antibodies, Monoclonal; D006241:Haptens; D007140:Immunoglobulin Fab Fragments; D010455:Peptides; D001564:Benzo(a)pyrene