Effect of double-stranded DNA on maturation of dendritic cells in vitro.
A preparation of human genomic fragmented double-stranded DNA (dsDNA) was used as maturation stimulus in cultures of human dendritic cells (DCs) generated in compliance with the interferon protocol. Culturing of the DCs in medium with 5μg/ml of the DNA preparation was associated with a decrease in the relative proportion of CD14 + cells and an increase in that of CD83 + cells. These changes are markers of DC maturation. The efficiency with which the DNA preparation was able to elicit DC maturation was commensurate with that of lypopolysaccharide from bacterial cell, the standard inducer of DC maturation. Generated ex vivo, matured in the presence of the human DNA preparation, pulsed with tumor antigens mouse DCs were used as a vaccine in biological tests for its antitumor activity. The experimental results demonstrate that reinfusion of mature pulsed with tumor antigens DCs cause a statistically significant suppression of tumor graft growth.
Alyamkina
Ekaterina A
EA
Novosibirsk State University, Russia.
Leplina
Olga Y
OY
Sakhno
Ludmila V
LV
Chernykh
Elena R
ER
Ostanin
Alexandr A
AA
Efremov
Yaroslav R
YR
Shilov
Alexandr G
AG
Proskurina
Anastasia S
AS
Orishchenko
Konstantin E
KE
Dolgova
Evgenia V
EV
Rogachev
Vladimir A
VA
Nikolin
Valeriy P
VP
Popova
Nelly A
NA
Zagrebelniy
Stanislav N
SN
Bogachev
Sergey S
SS
Shurdov
Mikhail A
MA
D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D000951:Antigens, Neoplasm / Q000276:immunology; D019496:Cancer Vaccines / Q000276:immunology / Q000627:therapeutic use; D002286:Carcinoma, Ehrlich Tumor / Q000276:immunology / Q000517:prevention & control; D002454:Cell Differentiation / Q000187:drug effects*; D004247:DNA / Q000494:pharmacology*; D003713:Dendritic Cells / Q000166:cytology* / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D005260:Female; D006801:Humans; D007136:Immunoglobulins / Q000378:metabolism; D053645:Interleukin-2 Receptor alpha Subunit / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D018950:Lipopolysaccharide Receptors / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D008562:Membrane Glycoproteins / Q000378:metabolism; D051379:Mice; D008808:Mice, Inbred CBA; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D023041:Xenograft Model Antitumor Assays; D000097802:CD83 Antigen
D015703:Antigens, CD; D000951:Antigens, Neoplasm; D019496:Cancer Vaccines; D004247:DNA; D007136:Immunoglobulins; D053645:Interleukin-2 Receptor alpha Subunit; D018950:Lipopolysaccharide Receptors; D008070:Lipopolysaccharides; D008562:Membrane Glycoproteins; D014409:Tumor Necrosis Factor-alpha; D000097802:CD83 Antigen; C508017:IL2RA protein, human