title: Comparison of the VITEK 2 system with the E-test for the determination of glycopeptide susceptibility of vanA and vanC positive enterococci.
abstract: The performance of the VITEK 2 System (bioMérieux) version 3.01 software was compared to that of the E-test (AB Biodisk, Solna, Sweden) for antibiotic susceptibility testing of 17 clinical isolates of vancomycin resistant enterococcus (VRE). Antibiotic Susceptibility Testing (AST) by VITEK 2 produced 10 minor (59%) errors, resulting in false phenotypes. Reporting of vancomycin resistance in enterococcal strains has enormous therapeutic and epidemiological consequences. Therefore, at laboratories using automated systems (e.g. VITEK 2) for routine microbiological susceptibility testing data must be confirmed by independent validated methods, e.g. E-test, or microdilution.
authors.affiliation: Debrecen University, Institute of Medical Microbiology, Medical and Health Science Center, Debrecen, Hungary. zsuzsanna.dombradi@gmail.com
authors.forename: Zsuzsanna
authors.initials: Z
authors.lastname: Dombrádi
authors.forename: Z
authors.initials: Z
authors.lastname: Bihari
authors.forename: Katalin Illésné
authors.initials: KI
authors.lastname: Horváth
authors.forename: Judit
authors.initials: J
authors.lastname: Szabó
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D015373:Bacterial Typing Techniques / Q000295:instrumentation / Q000379:methods*; D019729:Carbon-Oxygen Ligases / Q000235:genetics / Q000378:metabolism*; D016983:Enterococcus / Q000145:classification / Q000187:drug effects / Q000201:enzymology / Q000302:isolation & purification*; D005838:Genotype; D006020:Glycopeptides / Q000494:pharmacology*; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000295:instrumentation / Q000379:methods*; D010453:Peptide Synthases / Q000235:genetics / Q000378:metabolism*; D012984:Software; D020713:Vancomycin Resistance
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D006020:Glycopeptides; C071437:VanA ligase, Bacteria; D019729:Carbon-Oxygen Ligases; D010453:Peptide Synthases; C108395:VanC protein, Enterococcus