Serum bisphenol A pharmacokinetics and prostate neoplastic responses following oral and subcutaneous exposures in neonatal Sprague-Dawley rats.
The present study examines BPA pharmacokinetics in neonatal rats following s.c. injection or oral delivery of 10 μg BPA/kg BW and compares susceptibility to estrogen-induced prostate intraepithelial neoplasia (PIN) following either exposure route. Serum BPA in PND3 rats was measured using HPLC-MS-MS. Free and total BPA at C(max) were 1.77 and 2.0 ng/ml, respectively following injection and 0.26 and 1.02 ng/ml, respectively following oral exposure. The AUC(0-2) for free and total BPA was 4.1-fold and 1.8-fold greater, respectively, in s.c. vs. oral delivery. While exposure route affected BPA metabolism, internal dosimetry following s.c. injection of 10 μg BPA/kg BW is similar to BPA levels observed in humans. Prostates from aged rats given s.c. or oral BPA neonatally and T+E implants as adults exhibited nearly identical, heightened susceptibility to PIN incidence and score as compared to neonatal oil-controls. These findings on prostate health are directly relevant to humans at current BPA exposure levels.
Prins
Gail S
GS
Department of Urology, 820 S Wood St, MC 955, University of Illinois at Chicago, Chicago, IL 60612, USA. gprins@uic.edu
Ye
Shu-Hua
SH
Birch
Lynn
L
Ho
Shuk-mei
SM
Kannan
Kurunthachalam
K
D000284:Administration, Oral; D000818:Animals; D000831:Animals, Newborn; D001559:Benzhydryl Compounds; D004968:Estrogens, Non-Steroidal / Q000097:blood / Q000493:pharmacokinetics* / Q000633:toxicity*; D007279:Injections, Subcutaneous; D008297:Male; D010636:Phenols / Q000097:blood / Q000493:pharmacokinetics* / Q000633:toxicity*; D019048:Prostatic Intraepithelial Neoplasia / Q000139:chemically induced* / Q000378:metabolism / Q000473:pathology; D011471:Prostatic Neoplasms / Q000139:chemically induced* / Q000378:metabolism / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D004968:Estrogens, Non-Steroidal; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds