Effect of induced oxidative stress and herbal extracts on acid phosphatase activity in lysosomal and microsomal fractions of midgut tissue of the silkworm, Bombyx mori.
Lysosomal and microsomal acid phosphatase activity was estimated in midgut tissue of silkworm larvae, Bombyx mori L. (Lepidoptera: Bombycidae), after induced oxidative stress by D-galactose. The larvae were simultaneously were treated with ethanolic extracts of Bacopa monniera and Lactuca sativa to study their antioxidant properties. Lipid peroxidation and fluorescence was measured to analyze extent of oxidative stress. The ethanolic extract of Lactuca sativa was found to be more effective in protecting membranes against oxidative stress than Bacopa monniera.
Gaikwad
Y B
YB
Department of Zoology, Shivaji University, Kolhapur, Maharashtra State, India. gaikwad347@rediffmail.com
Gaikwad
S M
SM
Bhawane
G P
GP
D000135:Acid Phosphatase / Q000378:metabolism*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D034681:Bacopa; D012831:Bombyx / Q000187:drug effects / Q000201:enzymology*; D004353:Drug Evaluation, Preclinical; D005690:Galactose; D041981:Gastrointestinal Tract / Q000187:drug effects / Q000201:enzymology; D018545:Lactuca; D015227:Lipid Peroxidation / Q000187:drug effects; D008247:Lysosomes / Q000201:enzymology; D008861:Microsomes / Q000201:enzymology; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*
D000975:Antioxidants; D010936:Plant Extracts; D000135:Acid Phosphatase; D005690:Galactose