title: [Simultaneous determination of five lignans in Schisandra chinensis by HPLC].
abstract: OBJECTIVE
  To develop a HPLC quantitative method for determination of schisandrin, schisandrol B, schisantherin A, deoxyschisandrin and gamma-schisandrin in Fructus Schisandrae.
  
  
  METHODS
  A symmetry C18 column (4.6 mm x 250 mm, 5 microm) was used with methanol (A) and water (B) as mobile phases, in gradient elution. The gradient program was as follows: 0-36 min, changed from 60% A to 66% A, 36-65 min, to 80% A, 65-70 min, kept for 80% A, 70-75 min, to 100% A. The flow rate was 0.5 mL x min(-1) and detection wavelength was set at 254 nm.
  
  
  RESULTS
  The linearities of schisandrin, schisandrol B, schisantherin A, deoxyschisandrin and gamma-schisandrin were in the ranges of 0.0214. 160 (r = 0.9999), 0.020-4.000 (r = 0.9999), 0.021-4.240 (r = 0.9999), 0.020-3.960 (r = 0.9999) and 0.021-4.200 (r = 0.9999). The average recoveries were 104.8%, 104.2%, 102.7%, 104.6%, 104.5%, respectively.
  
  
  CONCLUSIONS
  The method developed in this study was reliable, and can be used for the quality control of the fruits of S. chinensis.
authors.affiliation: College of Horticulture, Shenyang Agriculture University, Shenyang 110161, China. dingzuo.1985@163.com
authors.forename: Zuo
authors.initials: Z
authors.lastname: Ding
authors.forename: Weiwei
authors.initials: W
authors.lastname: Lu
authors.forename: Hongbo
authors.initials: H
authors.lastname: Li
authors.forename: Junfan
authors.initials: J
authors.lastname: Fu
authors.forename: Zhongyun
authors.initials: Z
authors.lastname: Piao
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D017705:Lignans / Q000032:analysis* / Q000302:isolation & purification; D010936:Plant Extracts / Q000032:analysis* / Q000302:isolation & purification; D032141:Schisandra / Q000737:chemistry*
chemical_list: D017705:Lignans; D010936:Plant Extracts