title: Immunohistochemical characterization of 13 canine renal cell carcinomas.
abstract: Canine renal cell carcinomas (RCCs) are uncommon aggressive tumors that occur mainly in middle-aged male dogs. Their histologic classification bears no relationship with prognosis, and little information is available concerning their immunohistochemical properties. In this retrospective study, formalin-fixed, paraffin-embedded tissues from 13 canine RCCs were retrieved from the archives, classified histologically, and evaluated immunohistochemically. The dogs were 7 males and 6 females (1 spayed) of 10 different breeds, averaging 8 years in age. The tumors were classified as papillary, tubulopapillary, papillary-cystic, solid, or sarcomatoid. All 13 tumors were immunohistochemically positive for uromodulin, 12 for c-KIT, 11 for vimentin, 9 for wide-spectrum-screening cytokeratins, 7 for cytokeratins AE1/AE3 and carcinoembryonic antigen, 4 for cytokeratins CAM 5.2, and 3 for CD10. All 3 solid RCCs expressed vimentin, c-KIT, and carcinoembryonic antigen and were negative for cytokeratins. All 7 papillary and tubulopapillary tumors expressed vimentin; 6 (86%), cytokeratins; and 6 (86%), c-KIT. Both papillary-cystic RCCs were positive for cytokeratins and c-KIT and negative for vimentin. These results indicate that the different histologic types of RCC have characteristic immunohistochemical profiles and that c-KIT may be involved in the pathogenesis of canine RCC.
authors.affiliation: Dept Patologia e Imunologia Molecular, Instituto de Ciências Biomédicas de Abel Salazar, Porto, Portugal.
authors.forename: R M
authors.initials: RM
authors.lastname: Gil da Costa
authors.forename: J P
authors.initials: JP
authors.lastname: Oliveira
authors.forename: A L
authors.initials: AL
authors.lastname: Saraiva
authors.forename: F
authors.initials: F
authors.lastname: Seixas
authors.forename: F
authors.initials: F
authors.lastname: Faria
authors.forename: F
authors.initials: F
authors.lastname: Gärtner
authors.forename: M A
authors.initials: MA
authors.lastname: Pires
authors.forename: C
authors.initials: C
authors.lastname: Lopes
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000378:metabolism; D002292:Carcinoma, Renal Cell / Q000145:classification / Q000473:pathology / Q000662:veterinary*; D004283:Dog Diseases / Q000473:pathology*; D004285:Dogs; D005260:Female; D007150:Immunohistochemistry / Q000662:veterinary; D007680:Kidney Neoplasms / Q000145:classification / Q000473:pathology / Q000662:veterinary*; D008297:Male; D019009:Proto-Oncogene Proteins c-kit / Q000378:metabolism; D012189:Retrospective Studies
chemical_list: D014408:Biomarkers, Tumor; D019009:Proto-Oncogene Proteins c-kit