[Development of the PCR-test system for detection primate betaherpesvirinae].
A PCR-test system for detection of primate Betaherpesvirinae viruses was developed. Using alignment of complete genome of human, chimpanzee, and macaque rhesus cytomegalovirus conserve regions of viral genes were found. The oligonucleotide primers for consensus conserve regions of CMV UL56 gene were developed. The conditions of PCR were optimized and primer specificity for cytomegaloviruses of different primate species was confirmed.
Agumava
A A
AA
Chikobava
M G
MG
Lapin
B A
BA
D000818:Animals; D001483:Base Sequence; D003587:Cytomegalovirus / Q000235:genetics / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000175:diagnosis / Q000662:veterinary*; D017931:DNA Primers / Q000235:genetics; D005260:Female; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D018419:Primate Diseases / Q000175:diagnosis* / Q000821:virology*; D012680:Sensitivity and Specificity; D016415:Sequence Alignment; D015678:Viral Structural Proteins / Q000235:genetics
D017931:DNA Primers; C090238:UL56 protein, cytomegalovirus; D015678:Viral Structural Proteins