Effect of feeding regimens on polyhydroxybutyrate production from food wastes by Cupriavidus necator.
We investigated the effects of different feeding regimens (1-pulse, stepwise, and continuous) of fermented food-waste liquid on polyhydroxybutyrate (PHB) production. The fermentation liquid was filtered with a membrane filter (pore size, 0.45 μm) to remove anaerobic microorganisms and solids and used as a carbon source for Cupriavidus necator. One-pulse feeding yielded the highest cell concentration of C. necator. However, the PHB concentration was higher in the stepwise- and continuous-feeding regimens. Therefore, the continuous-feeding regimen was used for continuous PHB production. PHB could be produced over 259 h (8 draw-fill cycles) with a maximal PHB content of 87%, but the PHB concentration and content decreased with an increase in the operation time.
Hafuka
Akira
A
Division of Environmental Engineering, Faculty of Engineering, Hokkaido University, Sapporo, Japan.
Sakaida
Kenji
K
Satoh
Hisashi
H
Takahashi
Masahiro
M
Watanabe
Yoshimasa
Y
Okabe
Satoshi
S
D001673:Biodegradation, Environmental; D019149:Bioreactors / Q000382:microbiology*; D049921:Cupriavidus / Q000378:metabolism*; D019649:Food Industry; D005516:Food Microbiology; D006885:Hydroxybutyrates / Q000302:isolation & purification / Q000378:metabolism*; D007220:Industrial Waste / Q000517:prevention & control*; D011091:Polyesters / Q000302:isolation & purification / Q000378:metabolism*; D000094122:Polyhydroxybutyrates
D006885:Hydroxybutyrates; D007220:Industrial Waste; D011091:Polyesters; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates