title: Cutaneous Malassezia microbiota of healthy subjects differ by sex, body part and season.
abstract: Malassezia is a component of normal cutaneous resident microbiota. The aim of this study was to quantitatively clarify the differences in cutaneous Malassezia microbiota in healthy subjects by sex, body part and season. Samples were collected from the forehead, cheek, upper chest and upper back of 20 healthy men and 20 healthy women (average age 32 years) in summer and winter by the swab method. Malassezia DNA was analyzed using a real-time PCR system. As a result, in sex, body parts and season, men, the upper trunk and summer showed the highest total numbers of cutaneous Malassezia species on average. There were also differences depending on the analytical method. The predominant species were M. restricta on the face of men, M. globosa and M. dermatis on the upper trunk of men, and M. globosa and M. sympodialis on the upper trunk of women. This study clarified that the cutaneous Malassezia microbiota of healthy subjects differed by sex, body part and season.
authors.affiliation: Department of Dermatology, Fujita Health University School of Medicine, Toyoake, Aichi, Japan. akaza.narifumi@menard.co.jp
authors.forename: Narifumi
authors.initials: N
authors.lastname: Akaza
authors.forename: Hirohiko
authors.initials: H
authors.lastname: Akamatsu
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Sasaki
authors.forename: Shiori
authors.initials: S
authors.lastname: Takeoka
authors.forename: Masataka
authors.initials: M
authors.lastname: Kishi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Mizutani
authors.forename: Akiyo
authors.initials: A
authors.lastname: Sano
authors.forename: Keiko
authors.initials: K
authors.lastname: Hirokawa
authors.forename: Satoru
authors.initials: S
authors.lastname: Nakata
authors.forename: Kayoko
authors.initials: K
authors.lastname: Matsunaga
mesh_terms: D000328:Adult; D001415:Back / Q000382:microbiology; D002610:Cheek / Q000382:microbiology; D004271:DNA, Fungal / Q000032:analysis; D005145:Face / Q000382:microbiology; D005260:Female; D005546:Forehead / Q000382:microbiology; D006801:Humans; D008290:Malassezia / Q000302:isolation & purification*; D008297:Male; D054892:Metagenome; D012621:Seasons; D012737:Sex Factors; D012867:Skin / Q000382:microbiology*; D013909:Thorax / Q000382:microbiology; D055815:Young Adult
chemical_list: D004271:DNA, Fungal