Control of cell function on a phospholipid polymer having phenylboronic acid moiety.
We synthesized a water-insoluble phospholipid polymer bearing a phenylboronic acid moiety (PMBV), which induces cell adhesion through a specific interaction with the glycoprotein, fibronectin. Surface plasmon resonance analysis revealed that fibronectin was adsorbed on the PMBV surface. When fibroblasts were cultured on the PMBV surface, the cells adhered and proliferated normally while showing a spherical morphology. In addition, the adherent cells were able to detach after the addition of sugar molecules, which bound to phenylboronic acid through an exchange reaction. The cell cycle of adherent cells was evaluated with the embedded HeLa-Fucci cells by using a fluorescent ubiquitination-based cell cycle indicator. The cell-cycle analysis by fluorescence microscopy indicated that the adherent HeLa-Fucci cells tended to converge to the G1 phase. The differentiation of mesenchymal stem cells to chondrocytes was accelerated on PMBV in the presence of bone morphogenetic protein-2. We concluded that PMBV is a useful surface in experiments for assessing cellular function and differentiation.
Saito
Aya
A
Department of Materials Engineering, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
Konno
Tomohiro
T
Ikake
Hiroki
H
Kurita
Kimio
K
Ishihara
Kazuhiko
K
D000327:Adsorption; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D055396:Bone Morphogenetic Protein 2 / Q000494:pharmacology; D001897:Boronic Acids / Q000737:chemistry*; D002448:Cell Adhesion / Q000502:physiology; D002454:Cell Differentiation; D002468:Cell Physiological Phenomena; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology; D005347:Fibroblasts / Q000166:cytology; D005353:Fibronectins / Q000737:chemistry / Q000378:metabolism; D016193:G1 Phase; D006367:HeLa Cells; D006801:Humans; D007739:L Cells; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology; D051379:Mice; D015394:Molecular Structure; D010743:Phospholipids / Q000737:chemistry*; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D020349:Surface Plasmon Resonance; D013499:Surface Properties
D001672:Biocompatible Materials; D055396:Bone Morphogenetic Protein 2; D001897:Boronic Acids; D005353:Fibronectins; D010743:Phospholipids; D011108:Polymers; C010686:benzeneboronic acid