title: Whole-mount in situ hybridization for analysis of gene expression during Aedes aegypti development.
abstract: Blood-feeding mosquitoes, including the dengue and yellow fever vector Aedes aegypti, transmit many of the world's deadliest diseases. Such diseases have resurged in developing countries and pose clear threats for epidemic outbreaks in developed countries. Recent mosquito genome projects have stimulated interest in the potential for arthropod-borne disease control by genetic manipulation of vector insects, and genes that regulate development are of particular interest. This protocol for whole-mount in situ hybridization can be used to analyze gene expression in Ae. aegypti embryos and larvae, a critical aspect of understanding developmental gene function in this vector mosquito.
authors.affiliation: Department of Medical and Molecular Genetics, Indiana University School of Medicine, South Bend, IN 46617, USA.
authors.forename: Morgan
authors.initials: M
authors.lastname: Haugen
authors.forename: Michael
authors.initials: M
authors.lastname: Tomchaney
authors.forename: Kristopher
authors.initials: K
authors.lastname: Kast
authors.forename: Ellen
authors.initials: E
authors.lastname: Flannery
authors.forename: Anthony
authors.initials: A
authors.lastname: Clemons
authors.forename: Caitlin
authors.initials: C
authors.lastname: Jacowski
authors.forename: Wendy
authors.initials: W
authors.lastname: Simanton Holland
authors.forename: Christy
authors.initials: C
authors.lastname: Le
authors.forename: David
authors.initials: D
authors.lastname: Severson
authors.forename: Molly
authors.initials: M
authors.lastname: Duman-Scheel
mesh_terms: D000330:Aedes / Q000235:genetics* / Q000254:growth & development*; D000818:Animals; D046508:Culture Techniques / Q000379:methods; D018507:Gene Expression Regulation, Developmental; D017403:In Situ Hybridization / Q000379:methods*; D007303:Insect Vectors / Q000235:genetics* / Q000254:growth & development*