A practical solution-phase synthesis of an antagonistic peptide of TNF-α based on hydrophobic tag strategy.
A simple acid-resistant hydrophobic tag, which can be removed rapidly in a single-step procedure after overall peptide synthesis, has been developed to accomplish practical solution-phase synthesis of a 15-mer antagonistic peptide of TNF-α (A-TNF-α). Hydrophobically tagged peptides can be separated as precipitates at each step by addition of a polar organic solvent.
Tana
Gegen
G
Laboratory of Bio-organic Chemistry, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu, Tokyo 183-8509, Japan.
Kitada
Shingo
S
Fujita
Shuji
S
Okada
Yohei
Y
Kim
Shokaku
S
Chiba
Kazuhiro
K
D000595:Amino Acid Sequence; D057927:Hydrophobic and Hydrophilic Interactions; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry; D012996:Solutions / Q000737:chemistry; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors* / Q000378:metabolism
D010455:Peptides; D012996:Solutions; D014409:Tumor Necrosis Factor-alpha