title: Resveratrol upregulated SIRT1, FOXO1, and adiponectin and downregulated PPARγ1-3 mRNA expression in human visceral adipocytes.
abstract: BACKGROUND
  The SIRT1 enzyme is involved in adipose tissue (AT) lipolysis. FOXO1 is a protein that plays a significant role in regulating metabolism. Adiponectin is an adipokine, secreted by the AT, which has been considered to have an antiobesity function. PPARγ is one of the key actors in adipocytes differentiation. This study was undertaken to investigate whether resveratrol can regulate SIRT1, FOXO1, adiponectin, PPARγ1-3, and PPARβ/δ in human AT.
  
  
  METHODS
  The effects of resveratrol were analyzed in freshly isolated adipocytes prepared from visceral fat tissue samples obtained during bariatric surgery. Genes messenger ribonucleic acid (mRNA) levels were determined by qRT-PCR.
  
  
  RESULTS
  Ours results show that resveratrol modulates the studied genes, increasing SIRT1 (p = 0.021), FOXO1 (p = 0.001), and adiponectin (p = 0.025) mRNA expression and decreasing PPARγ1-3 (p = 0.003) mRNA in human visceral adipocytes.
  
  
  CONCLUSIONS
  Resveratrol, in vitro and at low concentration, modulates genes that are related to lipid metabolism, possibly preventing metabolic disease in human visceral adipose tissue (VAT).
authors.affiliation: Departamento de Bioquímica, ICBS, UFRGS, Rua Ramiro Barcelos 2600 Anexo, 90035-003, Porto Alegre, RS, Brazil.
authors.forename: Cíntia dos Santos
authors.initials: Cdos S
authors.lastname: Costa
authors.forename: Francieli
authors.initials: F
authors.lastname: Rohden
authors.forename: Thais Ortiz
authors.initials: TO
authors.lastname: Hammes
authors.forename: Rogério
authors.initials: R
authors.lastname: Margis
authors.forename: Josiane Woutheres
authors.initials: JW
authors.lastname: Bortolotto
authors.forename: Alexandre Vontobel
authors.initials: AV
authors.lastname: Padoin
authors.forename: Cláudio Cora
authors.initials: CC
authors.lastname: Mottin
authors.forename: Regina Maria
authors.initials: RM
authors.lastname: Guaragna
mesh_terms: D017667:Adipocytes / Q000378:metabolism*; D052242:Adiponectin / Q000235:genetics / Q000378:metabolism*; D000328:Adult; D015536:Down-Regulation / Q000187:drug effects; D000071161:Forkhead Box Protein O1; D051858:Forkhead Transcription Factors / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D050152:Intra-Abdominal Fat / Q000166:cytology; D050356:Lipid Metabolism / Q000187:drug effects*; D057847:Lipid Regulating Agents / Q000494:pharmacology*; D009767:Obesity, Morbid / Q000235:genetics / Q000378:metabolism* / Q000601:surgery; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D000077185:Resveratrol; D020133:Reverse Transcriptase Polymerase Chain Reaction; D056564:Sirtuin 1 / Q000235:genetics / Q000378:metabolism*; D013267:Stilbenes / Q000494:pharmacology*; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D052242:Adiponectin; C085126:FOXO1 protein, human; D000071161:Forkhead Box Protein O1; D051858:Forkhead Transcription Factors; D057847:Lipid Regulating Agents; D047495:PPAR gamma; D012333:RNA, Messenger; D013267:Stilbenes; C447939:SIRT1 protein, human; D056564:Sirtuin 1; D000077185:Resveratrol