Undetectable HIV-1 RNA load with the Cobas TaqMan v1.0 in a patient diagnosed at the time of primary HIV-1 infection.
Diagnosis of primary HIV-1 infection is challenging due to the presence of a serological window; thus, HIV-1-RNA quantitation and/or measurement of p24 antigenemia are recommended in such cases. A patient was diagnosed at the time of primary HIV-1 infection, he harbored a CFR02_AG subtype virus; quantitation of plasma HIV-1-RNA yielded an undetectable result according to one commercial assay, while HIV-1-RNA was detectable when measured with three other assays.
Ghosn
Jade
J
Laboratoire de Virologie, Université Paris Descartes, CHU Necker-Enfants Malades, 149, Rue de Sèvres, 75015 Paris, France. jade.ghosn@bct.aphp.fr
Galimand
Julie
J
Meyer
Laurence
L
Descamps
Diane
D
Vabret
Astrid
A
Deveau
Christiane
C
Yéni
Patrick
P
Goujard
Cécile
C
Rouzioux
Christine
C
Chaix
Marie-Laure
ML
D015658:HIV Infections / Q000175:diagnosis* / Q000821:virology; D015497:HIV-1 / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D012367:RNA, Viral / Q000097:blood*; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D019914:Taq Polymerase; D019562:Viral Load
D012367:RNA, Viral; D011933:Reagent Kits, Diagnostic; D019914:Taq Polymerase