Immunohistochemical localization of oestrogen receptor alpha in the various cell categories of chick embryo ovary.
The immunohistochemical (IHC) localization of oestrogen receptor alpha (ERα) was studied in the developing left ovary of 14.5-day-old chick embryos. The study was focused in particular on distinguishing in cortex and medulla the different cell categories that proved positive to the reaction, in order to gain further understanding of gonadal cell interactions during ovarian development. Immunostained cells were observed in both the cortex and medulla, but the reactivity for ERα was discontinuous, probably due to variable cell requirements. In the cortex, positivity was observed in cells of the ovarian surface epithelium, in germ cells and in prefollicular cells. In the medulla, positivity was found in the following cell categories: interstitial cells, poorly differentiated somatic cord cells, including those delimiting lacunae, germ cells and their accompanying cells of epithelial origin. Furthermore, the IHC results showed that the intracellular localization of the antigen was cytoplasmic, nuclear, or both. The significance of ERα presence and intracellular localization was discussed in relation and as supplementary to previous research by various Authors. In particular, as regards the unusual cytoplasmic immunoreactivity, a gradual shift of ERα localization from cytoplasmic to nuclear during the embryonic period is suggested.
Civinini
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A
Department of Biology and Biotechnologies, Charles Darwin University of Rome, Viale dell'Università 32, 00185 Rome, Italy.
Chimenti
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C
Gallo
V P
VP
D000818:Animals; D000911:Antibodies, Monoclonal; D002642:Chick Embryo / Q000737:chemistry* / Q000196:embryology; D004847:Epithelial Cells / Q000737:chemistry / Q000166:cytology / Q000648:ultrastructure; D047628:Estrogen Receptor alpha / Q000032:analysis*; D005260:Female; D005854:Germ Cells / Q000737:chemistry / Q000166:cytology / Q000648:ultrastructure; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D010053:Ovary / Q000737:chemistry* / Q000166:cytology / Q000196:embryology* / Q000648:ultrastructure; D013799:Theca Cells / Q000166:cytology / Q000648:ultrastructure
D000911:Antibodies, Monoclonal; D047628:Estrogen Receptor alpha