title: Antimutagenic potential of glucosinolate-rich seed extracts of broccoli (Brassica oleracea L var italica Plenck).
abstract: In the current study, isolation of glucosinolate degradation products was done in 4 different incubation solutions with different pHs based on the fact that distinct hydrolytic products are formed at different pHs. All the extracts were tested against direct-acting mutagens (4 nitro-o-phenylenediamine [NPD]), sodium azide, and indirect-acting mutagen (2-aminofluorene [2AF]). It was observed that extracts inhibited mutagenesis induced by the S9-dependent mutagen (2AF) more significantly than direct-acting mutagens. Two different modes of experimentation (pre-incubation and co-incubation) were used, and it was observed that the extracts showed better results in the pre-incubation mode of experimentation. Out of the 4 extracts tested, 0.1 mol/L of HCl extract was found to be the most effective in inhibiting mutagenesis with both TA 98 and TA 100 strains of Salmonella typhimurium. All other extracts also showed pronounced antimutagenic potential. The results of this study indicate the presence of potent antigenotoxic factors in broccoli, which are being explored further for their mechanism of action.
authors.affiliation: Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar-143005, Punjab, India.
authors.forename: Geetanjali
authors.initials: G
authors.lastname: Rampal
authors.forename: Tarunpreet Singh
authors.initials: TS
authors.lastname: Thind
authors.forename: Adarsh Pal
authors.initials: AP
authors.lastname: Vig
authors.forename: Saroj
authors.initials: S
authors.lastname: Arora
mesh_terms: D000818:Animals; D016587:Antimutagenic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D001937:Brassica / Q000737:chemistry*; D005449:Fluorenes / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000633:toxicity; D016368:Frameshift Mutation; D005961:Glucosinolates / Q000302:isolation & purification / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D008099:Liver / Q000378:metabolism; D008297:Male; D016296:Mutagenesis / Q000187:drug effects; D009152:Mutagenicity Tests / Q000379:methods; D009153:Mutagens / Q000378:metabolism / Q000633:toxicity; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D017354:Point Mutation; D051381:Rats; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics; D012639:Seeds / Q000737:chemistry*
chemical_list: D016587:Antimutagenic Agents; D005449:Fluorenes; D005961:Glucosinolates; D009153:Mutagens; D010936:Plant Extracts; C012177:2-aminofluorene