Genotyping of Toxoplasma gondii isolates with 15 microsatellite markers in a single multiplex PCR assay.
We developed an easy-to-use method for genotyping Toxoplasma gondii isolates in a single multiplex PCR assay with 15 microsatellite markers. This method was validated by testing 26 reference isolates that had been characterized with other sets of markers.
Ajzenberg
Daniel
D
Centre National de Référence (CNR) Toxoplasmose, Laboratoire de Parasitologie et de Mycologie, Centre Hospitalier-Universitaire Dupuytren, Faculté de Médecine, Université de Limoges, 2 avenue Martin Luther King, 87042 Limoges, France. ajz@unilim.fr
Collinet
Frédéric
F
Mercier
Aurélien
A
Vignoles
Philippe
P
Dardé
Marie-Laure
ML
D005838:Genotype; D018895:Microsatellite Repeats; D010274:Parasitology / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D014122:Toxoplasma / Q000145:classification* / Q000235:genetics*