Effect of N-acetylcysteine and fructose-1,6-bisphosphate in the treatment of experimental sepsis.
Sepsis is a syndrome caused by uncontrolled systemic inflammatory response of the individual, which represents a serious epidemiological problem worldwide. The aim of this study was to investigate the effect of N-acetylcysteine (NAC) and fructose-1,6-bisphosphate (FBP) in the treatment of experimental sepsis. We used rats that were divided into five experimental groups: normal control (not induced), septic control (induced using a capsule with non sterile fecal content and Escherichia coli), treated with FBP (500 mg/kg i.p.), treated with NAC (150 mg/kg i.p.), and treated with the combination of FBP with NAC. In the group treated with NAC, 16.68% of the mice survived, the FBP reduced the mortality of mice during the acute stage of the disease and increased the animals' survival time in 33.34%, and the combination of drugs had no effect. Our results show that NAC prevented the mortality of animals after septic induction. These data confirm the validity of the use of NAC in the treatment of sepsis. Our data also show that the synergistic action with FBP does not improve the picture.
de Mello
Ricardo Obalski
RO
Laboratório de Pesquisa em Biofísica Celular e Inflamação, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Avenida Ipiranga 6681, prédio 12C, sala 263, CEP 90.619-900, Porto Alegre, Rio Grande do Sul, Brazil.
Lunardelli
Adroaldo
A
Caberlon
Eduardo
E
de Moraes
Cristina Machado Bragança
CM
Christ Vianna Santos
Roberto
R
da Costa
Vinicius Lorini
VL
da Silva
Gabriela Viegas
GV
da Silva Scherer
Patrícia
P
Buaes
Luiz Eduardo Coimbra
LE
da Silva Melo
Denizar Alberto
DA
Donadio
Márcio Vinícius Fagundes
MV
Nunes
Fernanda Bordignon
FB
de Oliveira
Jarbas Rodrigues
JR
D000111:Acetylcysteine / Q000494:pharmacology / Q000627:therapeutic use*; D000818:Animals; D004357:Drug Synergism; D004359:Drug Therapy, Combination; D006597:Fructose-Bisphosphatase / Q000494:pharmacology / Q000627:therapeutic use*; D051381:Rats; D018805:Sepsis / Q000188:drug therapy* / Q000401:mortality; D015996:Survival Rate; D016896:Treatment Outcome
D006597:Fructose-Bisphosphatase; D000111:Acetylcysteine