Comparison of the VITEK 2 system with the E-test for the determination of glycopeptide susceptibility of vanA and vanC positive enterococci.
The performance of the VITEK 2 System (bioMérieux) version 3.01 software was compared to that of the E-test (AB Biodisk, Solna, Sweden) for antibiotic susceptibility testing of 17 clinical isolates of vancomycin resistant enterococcus (VRE). Antibiotic Susceptibility Testing (AST) by VITEK 2 produced 10 minor (59%) errors, resulting in false phenotypes. Reporting of vancomycin resistance in enterococcal strains has enormous therapeutic and epidemiological consequences. Therefore, at laboratories using automated systems (e.g. VITEK 2) for routine microbiological susceptibility testing data must be confirmed by independent validated methods, e.g. E-test, or microdilution.
Dombrádi
Zsuzsanna
Z
Debrecen University, Institute of Medical Microbiology, Medical and Health Science Center, Debrecen, Hungary. zsuzsanna.dombradi@gmail.com
Bihari
Z
Z
Horváth
Katalin Illésné
KI
Szabó
Judit
J
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D015373:Bacterial Typing Techniques / Q000295:instrumentation / Q000379:methods*; D019729:Carbon-Oxygen Ligases / Q000235:genetics / Q000378:metabolism*; D016983:Enterococcus / Q000145:classification / Q000187:drug effects / Q000201:enzymology / Q000302:isolation & purification*; D005838:Genotype; D006020:Glycopeptides / Q000494:pharmacology*; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000295:instrumentation / Q000379:methods*; D010453:Peptide Synthases / Q000235:genetics / Q000378:metabolism*; D012984:Software; D020713:Vancomycin Resistance
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D006020:Glycopeptides; C071437:VanA ligase, Bacteria; D019729:Carbon-Oxygen Ligases; D010453:Peptide Synthases; C108395:VanC protein, Enterococcus