Influence of cigarette smoke condensate on cariogenic and candidal biofilm formation on orthodontic materials.
BACKGROUND
An experimental analysis was made to quantify the adherence rates and the biofilm formation capacity of Streptococcus mutans ATCC25175 and Candida albicans SC5314 on orthodontic material surfaces in the presence of cigarette smoke condensate (CSC).


METHODS
Metal brackets, bands, acrylic resin, and polyurethane elastic rings were coated with stimulated saliva and submitted to adhesion and biofilm formation tests with and without CSC in a dynamic system.


RESULTS
The CSC increased the adhesion of S mutans ATCC25175 to the acquired pellicle (P <0.05) for bands (4.08 times), acrylic resin (2.89 times), and brackets (3.37 times) and reduced it in polyurethane elastic (2.66 times; P <0.05). S mutans ATCC25175 biofilm biomass was increased by CSC only on brackets (1.60 times; P <0.05). In the presence of CSC, the adhesion of C albicans SC5314 increased (P <0.05) on bands (1.81 times), brackets (9.61 times), elastics (29,133 times), and acrylic resin (177 times). Greater formation of C albicans SC5314 biofilm caused by CSC (P <0.05) was observed on acrylic resin (2.13 times) and brackets (2.32 times).


CONCLUSIONS
The results indicated that cigarette tobacco smoke can interfere with the adhesion and biofilm formation of these microorganisms to various orthodontic materials.
Baboni
Fernanda Brasil
FB
Postgraduate student, Graduate Program in Dentistry-Orthodontics, Pontifical Catholic University of Paraná, Curitiba, Paraná, Brazil.
Guariza Filho
Odilon
O
Associate professor, Graduate Program in Dentistry-Orthodontics, Pontifical Catholic University of Paraná, Curitiba, Paraná, Brazil.
Moreno
Andréa Novais
AN
Associate professor, Graduate Program in Health Sciences, Pontifical Catholic University of Paraná, Curitiba, Paraná, Brazil.
Rosa
Edvaldo Antonio Ribeiro
EAR
Professor, Graduate Program in Dentistry-Oral Biosciences, Pontifical Catholic University of Paraná, Curitiba, Paraná, Brazil. Electronic address: edvaldo.rosa@pucpr.br.
D000180:Acrylic Resins; D000328:Adult; D001422:Bacterial Adhesion; D018441:Biofilms; D002176:Candida albicans / Q000502:physiology; D015169:Colony Count, Microbial; D020724:Elastomers; D005260:Female; D006801:Humans; D008297:Male; D008422:Materials Testing; D009967:Orthodontic Appliances / Q000382:microbiology*; D012463:Saliva / Q000382:microbiology; D012906:Smoke; D013193:Stainless Steel; D018709:Statistics, Nonparametric; D013295:Streptococcus mutans / Q000502:physiology
D000180:Acrylic Resins; D020724:Elastomers; D012906:Smoke; D013193:Stainless Steel