title: [General retention time formulae for gradient liquid chromatography with any combination of isocratic, linear and stepwise gradients].
abstract: The ideal model of liquid chromatography in gradient elution was solved by using the characteristic approach on the assumption of a linear solvent strength model. By considering the influence of the dwelling time of the chromatographic system on retention time, the retention time formulae suited for any combination of isocratic, linear and stepwise gradients were obtained. It was found that the values of the retention time obtained from these formulae were in well accordance with those computed by using the finite-difference method, which confirms the validity of the formulae. Due to the simplicity and broad scope of applications, these formulae can be easily applied in practice.
authors.affiliation: Changzhou High-Tech Research Institute of Nanjing University, Changzhou 213164, China. hao_w_q@163.com
authors.forename: Weiqiang
authors.initials: W
authors.lastname: Hao
authors.forename: Bin
authors.initials: B
authors.lastname: Di
authors.forename: Yongbing
authors.initials: Y
authors.lastname: Yang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Chen
authors.forename: Junde
authors.initials: J
authors.lastname: Wang
mesh_terms: D000465:Algorithms; D002853:Chromatography, Liquid / Q000379:methods*; D008433:Mathematics; D008962:Models, Theoretical