Fast analysis of isoflavones by high-performance liquid chromatography using a column packed with fused-core particles.
The recent development of fused-core technology in HPLC columns is enabling faster and highly efficient separations. This technology was evaluated for the development of an fast analysis method for the most relevant soy isoflavones. A step-by-step strategy was used to optimize temperature (25-50°C), flow rate (1.2-2.7 mL/min), mobile phase composition and equilibration time (1-5 min). Optimized conditions provided a method for the separation of all isoflavones in less than 5.8 min and total analysis time (sample-to-sample) of 11.5 min. Evaluation of chromatographic performance revealed excellent reproducibility, resolution, selectivity, peak symmetry and low limits of detection and quantification levels. The use of a fused-core column allows highly efficient, sensitive, accurate and reproducible determination of isoflavones with an outstanding sample throughout and resolution. The developed method was validated with different soy samples with a total isoflavone concentration ranging from 1941.53 to 2460.84 μg g(-1) with the predominant isoflavones being isoflavone glucosides and malonyl derivatives.
Manchón
N
N
Centro para la Calidad de los Alimentos, Instituto Nacional de Investigación Y Tecnología Agraria y Alimentaria (INIA), C/José Tudela s/n, 42004 Soria, Spain.
D'Arrigo
M
M
García-Lafuente
A
A
Guillamón
E
E
Villares
A
A
Ramos
A
A
Martínez
J A
JA
Rostagno
M A
MA
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D007529:Isoflavones / Q000032:analysis*; D015203:Reproducibility of Results; D030262:Soybean Proteins / Q000032:analysis; D013025:Glycine max / Q000737:chemistry*; D013696:Temperature; D013997:Time Factors
D007529:Isoflavones; D030262:Soybean Proteins