title: Latency of herpes simplex virus type-1 in human geniculate and vestibular ganglia is associated with infiltration of CD8+ T cells.
abstract: Herpes simplex virus type-1 latency and CD8+ T-cell occurrence were investigated in the trigeminal, geniculate, and vestibular ganglia from seven deceased humans. The HSV-1 "latency-associated transcript" was assessed by in situ hybridization and quantitative RT-PCR. Infiltration of CD8+ T cell was detected by immunohistochemistry and quantitative RT-PCR. The data show that HSV-1 latency and CD8+ T-cell infiltration are not solely confined to the trigeminal ganglia but can also occur in other cranial ganglia along the neuroaxis. However, the HSV-1 latency transcripts in the geniculate and vestibular ganglia were expressed at a very low level. The difference in CD8 transcript levels among HSV-1 latently infected trigeminal ganglia, geniculate, and vestibular ganglia was less conspicuous. Colocalization of latent HSV-1 and CD8+ T cells in geniculate and vestibular ganglia supports further the hypothesis that HSV-1 reactivation is possible in these ganglia and is the cause of Bell's palsy and vestibular neuritis.
authors.affiliation: Clinic of Neurology am Marienplatz, Munich, Germany.
authors.forename: Viktor
authors.initials: V
authors.lastname: Arbusow
authors.forename: Tobias
authors.initials: T
authors.lastname: Derfuss
authors.forename: Kathrin
authors.initials: K
authors.lastname: Held
authors.forename: Susanne
authors.initials: S
authors.lastname: Himmelein
authors.forename: Michael
authors.initials: M
authors.lastname: Strupp
authors.forename: Robert
authors.initials: R
authors.lastname: Gurkov
authors.forename: Thomas
authors.initials: T
authors.lastname: Brandt
authors.forename: Diethilde
authors.initials: D
authors.lastname: Theil
mesh_terms: D000328:Adult; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D005830:Geniculate Ganglion / Q000276:immunology / Q000821:virology*; D018259:Herpesvirus 1, Human / Q000276:immunology / Q000502:physiology*; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007223:Infant; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014725:Vestibular Nerve / Q000276:immunology / Q000821:virology*; D017735:Virus Latency / Q000235:genetics / Q000502:physiology
chemical_list: D035683:MicroRNAs; C477253:latency associated transcript, herpes simplex virus-1