Local and cardiorenal effects of periodontitis in nitric oxide-deficient hypertensive rats.
OBJECTIVE
in this study we have assessed the renal and cardiac consequences of ligature-induced periodontitis in both normotensive and nitric oxide (NO)-deficient (L-NAME-treated) hypertensive rats.


METHODS
oral L-NAME (or water) treatment was started two weeks prior to induction of periodontitis. Rats were sacrificed 3, 7 or 14 days after ligature placement, and alveolar bone loss was evaluated radiographically. Thiobarbituric reactive species (TBARS; a lipid peroxidation index), protein nitrotyrosine (NT; a marker of protein nitration) and myeloperoxidase activity (MPO; a neutrophil marker) were determined in the heart and kidney.


RESULTS
in NO-deficient hypertensive rats, periodontitis-induced alveolar bone loss was significantly diminished. In addition, periodontitis-induced cardiac NT elevation was completely prevented by L-NAME treatment. On the other hand L-NAME treatment enhanced MPO production in both heart and kidneys of rats with periodontitis. No changes due to periodontitis were observed in cardiac or renal TBARS content.


CONCLUSIONS
in addition to mediating alveolar bone loss, NO contributes to systemic effects of periodontitis in the heart and kidney.
Herrera
Bruno S
BS
Department of Pharmacology, ICB, USP, São Paulo, SP, Brazil.
Martins-Porto
Rodrigo
R
Campi
Paula
P
Holzhausen
Marinella
M
Teixeira
Simone A
SA
Mendes
Gustavo D
GD
Costa
Soraia K P
SK
Gyurko
Robert
R
Van Dyke
Thomas E
TE
Spolidório
Luis C
LC
Muscará
Marcelo N
MN
D016301:Alveolar Bone Loss / Q000000981:diagnostic imaging / Q000378:metabolism; D000818:Animals; D004195:Disease Models, Animal; D004791:Enzyme Inhibitors / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000037:antagonists & inhibitors*; D006321:Heart / Q000187:drug effects; D006973:Hypertension / Q000378:metabolism*; D007668:Kidney / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009569:Nitric Oxide / Q000037:antagonists & inhibitors*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors; D018384:Oxidative Stress / Q000502:physiology; D010518:Periodontitis / Q000000981:diagnostic imaging / Q000378:metabolism*; D009195:Peroxidase / Q000032:analysis; D011859:Radiography; D051381:Rats; D017208:Rats, Wistar; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis; D014443:Tyrosine / Q000031:analogs & derivatives / Q000032:analysis
D004791:Enzyme Inhibitors; D016166:Free Radical Scavengers; D017392:Thiobarbituric Acid Reactive Substances; D009569:Nitric Oxide; C002744:3-nitrotyrosine; D014443:Tyrosine; D009195:Peroxidase; D019001:Nitric Oxide Synthase; D019331:NG-Nitroarginine Methyl Ester