Cellular compatibility of RGD-modified chitosan nanofibers with aligned or random orientation.
Aligned and randomly oriented chitosan nanofibers were prepared by electrospinning. The fibers were modified with the RGD cell-adhesive peptide through a heterobifunctional crosslinker containing a segment of poly(ethylene glycol) (PEG). PEG rendered the surface hydrophilic and provided flexible spacers, allowing the preservation of the bioactivity of further captured RGD peptides. NIH 3T3 cells were used to test the cellular compatibility of these chitosan nanofibrous scaffolds. Cell morphology and viability were investigated by SEM, fluorescent staining and cell counting. The results indicate that RGD-modified surfaces significantly improve the cellular compatibility of chitosan nanofibers and suggest a good candidate as a scaffold employed in tissue engineering.
Wang
Yan-Yan
YY
State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, People's Republic of China.
Lü
Lan-Xin
LX
Feng
Zhang-Qi
ZQ
Xiao
Zhong-Dang
ZD
Huang
Ning-Ping
NP
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002448:Cell Adhesion; D002452:Cell Count; D002470:Cell Survival; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry*; D005347:Fibroblasts / Q000648:ultrastructure; D051379:Mice; D015394:Molecular Structure; D008970:Molecular Weight; D041681:NIH 3T3 Cells; D057139:Nanofibers / Q000737:chemistry*; D009842:Oligopeptides / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000138:chemical synthesis / Q000737:chemistry; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry
D001672:Biocompatible Materials; D009842:Oligopeptides; D011092:Polyethylene Glycols; C047981:arginyl-glycyl-aspartic acid; D048271:Chitosan