Immunoliposomal systems targeting primary effusion lymphoma: in vitro study.
OBJECTIVE
To develop an appropriate liposomal formulation for gene delivery against primary effusion lymphoma (PEL), a herpesvirus HHV8-associated B-cell lymphoma.


METHODS
Cationic, cationic PEGylated and cationic PEGylated anti-CD138 liposomes (ILp) linking a monoclonal antibody expressed on PEL cells were prepared by a thin layer evaporation method, followed by extrusion technique. The formulations were mixed with a model oligonucleotide to form the lipoplexes and tested on a BCBL-1 cell (a PEL cell line). The transfection efficiency was evaluated by flow cytometry and confocal laser scanning microscopy analysis.


RESULTS
Based on antigen-antibody interaction, ILp mediated a specific gene delivery as shown by a significant increase in the transfection rate and a localized internalization of the oligonucleotide, in comparison with cationic liposomes and cationic PEGylated liposomes.


CONCLUSIONS
ILp could be proposed as effective carriers for oligonucleotide transfer in BCBL-1 cells. In vitro experimental results encourage us to further test the in vivo therapeutic potential of ILp for specific delivery of antitumoral agents.
Ruozi
Barbara
B
Department of Pharmaceutical Sciences, University of Modena & Reggio Emilia, Via Campi 183, Modena, Italy. barbara.ruozi@unimo.it
Riva
Giovanni
G
Belletti
Daniela
D
Tosi
Giovanni
G
Barozzi
Patrizia
P
Luppi
Mario
M
Forni
Flavio
F
Vandelli
Maria Angela
MA
D001483:Base Sequence; D045744:Cell Line, Tumor; D004587:Electrophoresis, Agar Gel; D005434:Flow Cytometry; D016650:Fluorescein-5-isothiocyanate; D006801:Humans; D066298:In Vitro Techniques; D008081:Liposomes; D054685:Lymphoma, Primary Effusion / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D018625:Microscopy, Atomic Force; D018613:Microscopy, Confocal
D008081:Liposomes; D016650:Fluorescein-5-isothiocyanate