Analysis of complete genomic sequences of isolates of the Sweet potato feathery mottle virus strains C and EA: molecular evidence for two distinct potyvirus species and two P1 protein domains.
The complete nucleotide sequence of the isolate C1 of Sweet potato feathery mottle virus (SPFMV) strain C and the 5' region of several other strains were determined and analyzed together with the sequences of isolates representing the EA, RC and O strains. This provided molecular evidence for the reclassification of SPFMV strains into two species and the occurrence of a complex recombinant isolate. Analysis also revealed a hypervariable domain in the P1 protein, which separates an N-terminal region unique to SPFMV and members of the ipomovirus species Sweet potato mild mottle virus from the C-terminal protease domain, which is conserved among all potyviruses.
Untiveros
Milton
M
Applied Biotechnology Laboratory, International Potato Center (CIP), Apartado 1558, Lima 12, Peru.
Quispe
Dora
D
Kreuze
Jan
J
D020121:5' Untranslated Regions; D016000:Cluster Analysis; D016679:Genome, Viral; D027723:Ipomoea batatas / Q000821:virology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D017800:Potyvirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D017434:Protein Structure, Tertiary; D012367:RNA, Viral / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014764:Viral Proteins / Q000235:genetics
D020121:5' Untranslated Regions; C409826:P1 protein, potyvirus; D012367:RNA, Viral; D014764:Viral Proteins