Redetermination of the X-ray structure of nitroxylcobalamin: base-on nitroxylcobalamin exhibits a remarkably long Co-N(dimethylbenzimidazole) bond distance.
The X-ray structures of three new crystals of nitroxylcobalamin (NOCbl) have been determined. Unlike our earlier reported structure in which NOCbl was partially oxidized (L. Hannibal, C. A. Smith, D. W. Jacobsen and N. E. Brasch, Angew. Chem., Int. Ed. 2007, 46, 5140), the O atom of the nitroxyl ligand is located in a single position with a N=O bond distance of 1.12-1.14 Å, consistent with a double bond. The Co-N-O angle is in the 118.9-120.3 Å range. The α-axial Co-N(dimethylbenzimidazole) (Co-NB3) bond distance is a remarkable 2.32-2.35 Å in length, ~0.1 Å longer than that reported for all other cobalamin structures. The change in the Gibbs free energy for the base-on/base-off equilibrium now correlates extremely well with the Co-NB3 bond distance, as observed for other cobalamins.
Hassanin
Hanaa A
HA
Department of Chemistry, Kent State University, Kent, OH 44242, USA.
El-Shahat
Mohamed F
MF
DeBeer
Serena
S
Smith
Clyde A
CA
Brasch
Nicola E
NE
D001562:Benzimidazoles / Q000737:chemistry*; D003035:Cobalt / Q000737:chemistry*; D009584:Nitrogen / Q000737:chemistry*; D009589:Nitrogen Oxides / Q000737:chemistry*; D009942:Organometallic Compounds / Q000737:chemistry*; D053838:Scattering, Small Angle; D014805:Vitamin B 12 / Q000737:chemistry*; D014961:X-Ray Diffraction
D001562:Benzimidazoles; D009589:Nitrogen Oxides; D009942:Organometallic Compounds; D003035:Cobalt; C015158:5,6-dimethylbenzimidazole; C039900:nitroxyl; D009584:Nitrogen; D014805:Vitamin B 12