A method for immediate comparative assessment of microinjected mammalian oocytes.
Manipulation of mammalian oocytes at the molecular level is hampered by low transcriptional activity and the presence of large stores of mRNA and protein. Microinjection of interfering macromolecules has become an important tool in studying oocyte maturation, although injection success, final concentrations of injected substances and viability after injection remain difficult to assess with current techniques. To address these problems, we developed an epifluorescence microscopy based technique to evaluate oocytes directly after (co-)injection of green fluorescent protein (GFP).
Hölzenspies
J J
JJ
Department of Farm Animal Health, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 104, 3584 CM Utrecht, the Netherlands.
Stoorvogel
W
W
Colenbrander
B
B
Roelen
B A J
BA
van Haeften
T
T
D000818:Animals; D002478:Cells, Cultured; D003584:Cytological Techniques / Q000379:methods; D005453:Fluorescence; D049452:Green Fluorescent Proteins / Q000008:administration & dosage / Q000494:pharmacology; D008322:Mammals; D008845:Microinjections / Q000009:adverse effects / Q000379:methods; D008856:Microscopy, Fluorescence; D009865:Oocytes / Q000166:cytology*; D030781:Organisms, Genetically Modified
D049452:Green Fluorescent Proteins