[Allele-specific chromosome 3p25 deletion detected by PCR-LOH analysis in non-small cell lung cancer.].
BACKGROUND
To ascertain whether the loss of heterozygosity (LOH) of allele on chromosome 3p25 region is associated with non-small cell lung cancer (NSCLC) .


METHODS
3p25 LOH was analysed in patients with NSCLC by polymerase chain reaction-based assays for dinucleatide repeat polymorphisms.


RESULTS
Eighty out of 158 (50. 6 %) cancer tissues of NSCLC were found to have 3p25 LOH , but no 3p25 LOH was observed in 5 normal fetal lung tissues and 4 tissues of the benign lung neoplasms. The deletion was detected in 62.0% of adenocarcinoma and 43.2% of the squamous carcinoma , indicating that the former was significantly higher than the latter ( P < 0. 05) . Loss frequences for TNM stages were further observed in 52.2% , 45.0% and 52.8% of the cases for stage I, II and III respectively , and these data showed no significant relationship between the loss frequence and TNM staging of NSCLC ( P > 0. 05) .


CONCLUSIONS
Above findings display that 3p25 LOH appear to be a common structural alteration occurred in NSCLC and there may be suppressor genes relevant to the carcinogenesis of the lung in 3p25 region.
Guan
S
S
Shanghai Cancer Institute, Shanghai 200032, P.R.China.
Xu
K
K
Liao
M
M
Chen
F
F