Curcumin promotes apoptosis in human lung adenocarcinoma cells through miR-186* signaling pathway.
Lung cancer is one of the leading causes of cancer-related death worldwide. Curcumin has been reported to have an antitumor effect by inducing apoptosis and suppressing growth of tumor cells. However, the mechanism by which curcumin exerts its anti-cancer effect needs further research. The purpose of the present study was to identify a miRNA-mediated mechanism which plays a role in the anti-cancer effects of curcumin. Alterations in miRNA expression were seen in curcumin-treated A549 cells, including significant downregulation of miRNA-186* expression by microarray analysis and real-time PCR. The miRNA-186* functions by overexpression or inhibition were investigated using biological assays in A549 cells. Additionally, caspase-10 was identified as a target of miRNA-186* using dual luciferase reporter assays and western blot analysis. These results demonstrate that curcumin induces A549 cell apoptosis through a miRNA pathway. Also, miRNA-186* could serve as a potential gene therapy target in curcumin treatment. furthermore, caspase-10 was shown to be a target of miR-186* regulation.
Zhang
Jian
J
Department of Respiratory Medicine, Xijing Hospital, The Fourth Military Medical University, Xi'an 710032, PR China. zhangjian197011@yahoo.com
Du
Yongping
Y
Wu
Changgui
C
Ren
Xinling
X
Ti
Xinyu
X
Shi
Jieran
J
Zhao
Feng
F
Yin
Hong
H
D020413:3' Untranslated Regions; D000230:Adenocarcinoma / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects* / Q000235:genetics; D001483:Base Sequence; D053455:Caspase 10 / Q000235:genetics; D048708:Cell Growth Processes / Q000187:drug effects / Q000235:genetics; D045744:Cell Line, Tumor; D003474:Curcumin / Q000494:pharmacology*; D015536:Down-Regulation; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000235:genetics; D046228:Microarray Analysis; D015398:Signal Transduction / Q000187:drug effects
D020413:3' Untranslated Regions; D000970:Antineoplastic Agents; C533096:MIRN186 microRNA, human; D035683:MicroRNAs; D053455:Caspase 10; D003474:Curcumin