Anti-resorptive effect of pilose antler blood (Cervus nippon Temminck) in ovariectomized rats.
Anti-bone resorption activity of pilose antler blood (Cervus nippon Temminck) were evaluated in ovariectomized Wistar rats. The rats were randomly divided into sham operated group (SHAM), ovariectomized group (OVX) and pilose antler blood treated group. The ovariectomized rats were treated with pilose antler blood orally in 4000 microl/kg daily doses for 10 weeks. Compared with SHAM group, serum 17 beta-estradiol level decreased significantly and osteocalcin level increased significantly in OVX group, indicating successful model of osteoporosis. The experiments showed that the bone mineral density of the lumbar spine and left femur in OVX group decreased remarkably compared to SHAM group but normalized by treatment with pilose antler blood. Additionally, serum levels of insulin-like growth factor-land testosterone were lower obviously in OVX group than those in SHAM group but preserved by pilose antler blood treatment. However, no obvious changes in serum levels of calcium, phosphorus, total alkaline phosphatase and osteoprotegerin were observed among three groups. These results suggested that administration of pilose antler blood was effective in alleviating osteoporosis in ovariectomized rats.
Yang
Jian-Hong
JH
School of Life Sciences, University of Science and Technology of China, Hefei 230026, China. yangjh@gucas.ac.cn
Cao
Yi
Y
Wang
Rui-Lin
RL
Fei
Yu-Rong
YR
Zhang
Hui
H
Feng
Pu
P
Liu
Jing
J
D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D000999:Antlers / Q000737:chemistry*; D001835:Body Weight / Q000187:drug effects; D015519:Bone Density / Q000187:drug effects; D001862:Bone Resorption / Q000188:drug therapy*; D002118:Calcium / Q000097:blood; D004958:Estradiol / Q000097:blood; D005260:Female; D008297:Male; D008419:Materia Medica / Q000302:isolation & purification / Q000494:pharmacology*; D015675:Osteocalcin / Q000097:blood; D010024:Osteoporosis / Q000188:drug therapy*; D010052:Ovariectomy; D010758:Phosphorus / Q000097:blood; D051381:Rats; D017208:Rats, Wistar
D008419:Materia Medica; D015675:Osteocalcin; D010758:Phosphorus; D004958:Estradiol; D000469:Alkaline Phosphatase; D002118:Calcium