title: Enzymatic synthesis of L-tryptophan from hair acid hydrolysis industries wastewater with tryptophan synthase.
abstract: An effective method for production of L-tryptophan from hair acid hydrolysis wastewater (HHW) containing L-serine was developed by recombinant tryptophan synthase. This study provides us with an alternative HHW utilization strategy. Tryptophan synthase could efficiently convert L-serine contained in HHW to L-tryptophan at pH 8.0, 40°C and Tween-80 of 0.04%. The enzyme also showed high tolerance to ammonium chloride, a component in HHW. In a scale up study, L-serine conversion rate reach 95.1% with a final L-tryptophan concentration of 33.2 g l(-1).
authors.affiliation: State Key Laboratory of Pharmaceutical Biotechnology, School of Life Science, Nanjing University, Nanjing, People's Republic of China.
authors.forename: Genhai
authors.initials: G
authors.lastname: Zhao
authors.forename: Junzhong
authors.initials: J
authors.lastname: Liu
authors.forename: Kai
authors.initials: K
authors.lastname: Dong
authors.forename: Fei
authors.initials: F
authors.lastname: Zhang
authors.forename: Hongjuan
authors.initials: H
authors.lastname: Zhang
authors.forename: Qian
authors.initials: Q
authors.lastname: Liu
authors.forename: Qingcai
authors.initials: Q
authors.lastname: Jiao
mesh_terms: D000143:Acids / Q000737:chemistry*; D006197:Hair / Q000737:chemistry*; D006868:Hydrolysis; D007220:Industrial Waste / Q000517:prevention & control*; D012694:Serine / Q000737:chemistry*; D014364:Tryptophan / Q000138:chemical synthesis*; D014367:Tryptophan Synthase / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000737:chemistry*
chemical_list: D000143:Acids; D007220:Industrial Waste; D014874:Water Pollutants, Chemical; D012694:Serine; D014364:Tryptophan; D014367:Tryptophan Synthase