Introduction to the field of enzyme immobilization and stabilization.
Enzyme stabilization is important for any biomedical or industrial application of enzymes. In many applications, the goal is to provide extended active lifetime at normal environmental conditions with traditional substrates at low concentrations in buffered solutions. However, as enzymes are used for more and more applications, there is a desire to use them in extreme environmental conditions (i.e., high temperatures), in high substrate concentration, and in nontraditional solvent systems. This chapter introduces the topic of enzyme stabilization and the methods used for enzyme stabilization including enzyme immobilization.
Moehlenbrock
Michael J
MJ
Department of Chemistry, Saint Louis University, St Louis, MO, USA.
Minteer
Shelley D
SD
D001709:Biotechnology / Q000379:methods* / Q000639:trends; D003432:Cross-Linking Reagents / Q000737:chemistry; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005976:Glutaral / Q000737:chemistry; D008958:Models, Molecular; D015394:Molecular Structure; D012997:Solvents / Q000737:chemistry*; D013696:Temperature
D003432:Cross-Linking Reagents; D004800:Enzymes, Immobilized; D012997:Solvents; D005976:Glutaral