Neuroprotective effect of honokiol and magnolol, compounds from Magnolia officinalis, on beta-amyloid-induced toxicity in PC12 cells.
Amyloid β peptide (Aβ) induced toxicity is a well-established pathway of neuronal cell death which might play a role in Alzheimer's disease. In this regard, the toxic effect of Aβ on a cultured Aβ-sensitive neuronal cell line was used as a primary screening tool for potential anti-Alzheimer's therapeutic agents. The effects of nine pure compounds (vitamin E, α-asarone, salidroside, baicolin, magnolol, gastrodin, bilobalide, honokiol and β-asarone) from selected Chinese herbs on neuronal cell death induced by Aβ in NGF-differentiated PC12 cells were examined. Only two of the studied compounds, honokiol and magnolol, significantly decreased Aβ-induced cell death. Further experiments indicated that their neuroprotective effects are possibly mediated through reduced ROS production as well as suppression of intracellular calcium elevation and inhibition of caspase-3 activity. The results provide for the first time a scientific rationale for the clinical use of honokiol and magnolol in the treatment of Alzheimer's disease.
Hoi
Chu Peng
CP
School of Pharmacy, The Chinese University of Hong Kong, Shatin, Hong Kong, China.
Ho
Yee Ping
YP
Baum
Larry
L
Chow
Albert H L
AH
D000544:Alzheimer Disease / Q000188:drug therapy; D016229:Amyloid beta-Peptides / Q000009:adverse effects; D000818:Animals; D017209:Apoptosis; D001713:Biphenyl Compounds / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism; D053148:Caspase 3 / Q000378:metabolism; D002470:Cell Survival; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D017705:Lignans / Q000494:pharmacology*; D031566:Magnolia / Q000737:chemistry*; D018696:Neuroprotective Agents / Q000494:pharmacology*; D016716:PC12 Cells; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D000498:Allyl Compounds; D010636:Phenols
D016229:Amyloid beta-Peptides; D001713:Biphenyl Compounds; D002118:Calcium; D053148:Caspase 3; D004365:Drugs, Chinese Herbal; D017705:Lignans; D018696:Neuroprotective Agents; D017382:Reactive Oxygen Species; C005498:magnolol; C005499:honokiol; D000498:Allyl Compounds; D010636:Phenols; C505410:Casp3 protein, rat