title: Activation of PKC-alpha is required for migration of C6 glioma cells.
abstract: The PKC signaling pathway has been implicated in diverse cellular functions. Here, we sought to investigate the role of PKC-alpha÷beta in C6 glioma cell migration. We found that both PKC-alpha and PKC-beta were expressed by C6 glioma cells, but only PKC-alpha was markedly activated in serum-treated C6 cells. Go6976, a PKC-alpha÷beta specific inhibitor, was found to cause a dose-dependent reduction of PKC-alpha activation and cell migration induced by serum in C6 cells. These results collectively indicated that the PKC-alpha signaling pathway is necessary for glioma cell migration. Our findings may provide an insight into a better understanding to the malignant progression of gliomas.
authors.affiliation: Anhui Key Laboratory of Tissue Transplantation, Bengbu Medical College, Bengbu, China. jghu.hzlv@yahoo.com.
authors.forename: Jian-Guo
authors.initials: JG
authors.lastname: Hu
authors.forename: Xiao-Fei
authors.initials: XF
authors.lastname: Wang
authors.forename: Jian-Sheng
authors.initials: JS
authors.lastname: Zhou
authors.forename: Feng-Chao
authors.initials: FC
authors.lastname: Wang
authors.forename: Xing-Wu
authors.initials: XW
authors.lastname: Li
authors.forename: He-Zuo
authors.initials: HZ
authors.lastname: Lü
mesh_terms: D000818:Animals; D000963:Antimetabolites; D015153:Blotting, Western; D001932:Brain Neoplasms / Q000378:metabolism / Q000473:pathology; D001973:Bromodeoxyuridine; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology*; D004789:Enzyme Activation; D005910:Glioma / Q000378:metabolism / Q000473:pathology; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D051571:Protein Kinase C-alpha / Q000037:antagonists & inhibitors / Q000378:metabolism*; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D051381:Rats
chemical_list: D000963:Antimetabolites; D047428:Protein Kinase Inhibitors; D051571:Protein Kinase C-alpha; D001973:Bromodeoxyuridine