title: Experimental studies of OH° radical/pressure dependence of arginase activity using a molecular chromatography approach.
abstract: Arginase is an enzyme which plays a role in pathophysiology such as hypertension. Here we demonstrated for the first time the direct implication of pressure and OH° radical formation on the arginase activity via a novel analytical procedure. Pressure increased arginase activity in the range 12-52bars. Activation by OH° radical showed a hyperbolic response. The OH° radicals produced were significantly inhibited by sulfasalazine (SAZ) and the inhibition of OH° radicals parallels the inhibition of arginase activity.
authors.affiliation: Laboratoire de Chimie Analytique et Physique EA4267, Faculté de Médecine Pharmacie, Université de Franche-Comté, Place Saint Jacques, 25030 Besançon Cedex, France.
authors.forename: Claire
authors.initials: C
authors.lastname: André
authors.forename: Firas
authors.initials: F
authors.lastname: Ibrahim
authors.forename: Tijani
authors.initials: T
authors.lastname: Gharbi
authors.forename: Guillaume
authors.initials: G
authors.lastname: Herlem
authors.forename: Yves Claude
authors.initials: YC
authors.lastname: Guillaume
mesh_terms: D000818:Animals; D001119:Arginase / Q000737:chemistry* / Q000378:metabolism; D019149:Bioreactors; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D017665:Hydroxyl Radical / Q000737:chemistry* / Q000494:pharmacology*; D007700:Kinetics; D011312:Pressure; D012460:Sulfasalazine / Q000494:pharmacology
chemical_list: D004800:Enzymes, Immobilized; D017665:Hydroxyl Radical; D012460:Sulfasalazine; D001119:Arginase