title: A chimeric peptide that binds to titanium and mediates MC3T3-E1 cell adhesion.
abstract: Our study provides a promising alternative of biomimetic coating which functionalizes the dental implant with adhesion peptides and may be useful for enhancing the bone remodeling around Ti implants. A chimeric peptide consisting of an Arg-Gly-Asp (RGD) sequence (mediating cell adhesion) and a RKLPDA (minTBP-1) sequence (specifically recognizing and binding to Ti substrate) was designed and synthesized. The chimeric peptide affinity to Ti disks, as well as its role in mediating MC3T3-E1 cell attachment and afterwards spreading on pre-coated Ti disks, was investigated. The chimeric peptide not only showed favorable affinity to Ti surfaces but also facilitated the adhesion of MC3T3-E1 cells.
authors.affiliation: Department of Stomatology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, No. 1095, Jiefang Road, Hankou District, Wuhan, 430030, Hubei, People's Republic of China.
authors.forename: Dan
authors.initials: D
authors.lastname: Wang
authors.forename: Jing
authors.initials: J
authors.lastname: Mao
authors.forename: Bin
authors.initials: B
authors.lastname: Zhou
authors.forename: Xiao-Fu
authors.initials: XF
authors.lastname: Liao
authors.forename: Shi-Qiang
authors.initials: SQ
authors.lastname: Gong
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.forename: Jing-Tao
authors.initials: JT
authors.lastname: Zhang
mesh_terms: D000818:Animals; D040761:Biomimetic Materials / Q000378:metabolism*; D002448:Cell Adhesion; D002460:Cell Line; D020099:Coated Materials, Biocompatible / Q000378:metabolism; D051379:Mice; D010006:Osteoblasts / Q000502:physiology*; D011485:Protein Binding; D011994:Recombinant Proteins / Q000378:metabolism*; D014025:Titanium / Q000378:metabolism*
chemical_list: D020099:Coated Materials, Biocompatible; D011994:Recombinant Proteins; D014025:Titanium