title: Evidence of closely related picobirnavirus strains circulating in humans and pigs in Argentina.
abstract: BACKGROUND
  On the basis of the published literature, it is still difficult to draw conclusions as to whether picobirnavirus (PBV) circulation is influenced by host species restriction.
  
  
  OBJECTIVE
  To provide data regarding the genetic relatedness between porcine and human PBV strains present in Argentina as a means of defining the host range and epidemiology of these viruses.
  
  
  METHODS
  Fecal specimens (n = 74) collected from kidney transplant patients (n = 55) and piglets (n = 19) were analyzed by RT-PCR using primers designed to amplify the porcine PBV genomic segment 2. Amplified sequences were further examined phylogenetically.
  
  
  RESULTS
  By RT-PCR amplification 14 of 74 samples rendered amplicons of the expected 282 base pair size (8 detected from humans and 6 from pigs). Eleven amplicons (5 from humans and 6 from pigs) were selected for sequencing and subjected to phylogenetic analysis. The eleven amplicons revealed similarities between human and porcine viral sequences that ranged between 94.7 and 100% in identity. Phylogenetic analysis identified these 11 strains as PBV genogroup I-related strains and showed that they grouped as a single separate clade distinct from other PBV strains detected in humans and porcine from other countries.
  
  
  CONCLUSIONS
  The present study suggests that closely related PBV strains infect both pigs and humans in Argentina and that the epidemiology of PBVs is not species restricted.
authors.affiliation: Instituto de Virología Dr. J.M. Vanella, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Enfermera Gordillo Gomez s/n, Agencia 4 e Ciudad Universitaria, Córdoba, Argentina.
authors.forename: Miguel O
authors.initials: MO
authors.lastname: Giordano
authors.forename: Laura C
authors.initials: LC
authors.lastname: Martinez
authors.forename: Gisela
authors.initials: G
authors.lastname: Masachessi
authors.forename: Patricia A
authors.initials: PA
authors.lastname: Barril
authors.forename: Leonardo J
authors.initials: LJ
authors.lastname: Ferreyra
authors.forename: María B
authors.initials: MB
authors.lastname: Isa
authors.forename: Mildre C
authors.initials: MC
authors.lastname: Valle
authors.forename: Pablo U
authors.initials: PU
authors.lastname: Massari
authors.forename: Silvia V
authors.initials: SV
authors.lastname: Nates
mesh_terms: D000818:Animals; D001118:Argentina / Q000453:epidemiology; D001483:Base Sequence; D003967:Diarrhea / Q000821:virology; D058507:Host Specificity / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D009710:Nucleotide Mapping; D010802:Phylogeny; D018708:Picobirnavirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D012327:RNA Virus Infections / Q000453:epidemiology* / Q000821:virology*; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017423:Sequence Analysis, RNA; D013552:Swine
chemical_list: D012324:RNA-Dependent RNA Polymerase