title: Mycobacterium massiliense BRA100 strain recovered from postsurgical infections: resistance to high concentrations of glutaraldehyde and alternative solutions for high level disinfection.
abstract: OBJECTIVE
  To evaluate the minimum inhibitory concentration (MIC) of GTA against these microorganisms and alternative disinfectants for high-level disinfection (HLD).
  
  
  METHODS
  Reference mycobacteria and clinical M. massiliense strains were included in this study. Active cultures were submitted to susceptibility qualitative tests with GTA dilutions (ranging from 1.5% to 8%), and commercial orthophthaldehyde (OPA) and peracetic acid (PA)-based solutions, during the period of exposure as recommended by National Agency of Sanitary Surveillance for HLD.
  
  
  RESULTS
  All reference and M. massiliense non-BRA100 strains, recovered from sputum, were susceptible to any GTA concentration, OPA and PA solutions. M. massiliense BRA100 strains presented MIC of 8% GTA and were susceptible to OPA and PA.
  
  
  CONCLUSIONS
  M. massiliense BRA100 strain is resistant to high GTA concentrations (up to 7%), which proves that this product is non-effective against specific rapidly growing mycobacteria and should be substituted by OPA or PA-based solutions for HLD.
authors.affiliation: Medical Sciences Faculty, Rio de Janeiro State University, Rio de Janeiro, Brazil.
authors.forename: Nádia Suely de Oliveira
authors.initials: NS
authors.lastname: Lorena
authors.forename: Marcos Bettini
authors.initials: MB
authors.lastname: Pitombo
authors.forename: Patrícia Barbur
authors.initials: PB
authors.lastname: Côrtes
authors.forename: Maria Cristina Araújo
authors.initials: MC
authors.lastname: Maya
authors.forename: Marlei Gomes da
authors.initials: MG
authors.lastname: Silva
authors.forename: Ana Carolina da Silva
authors.initials: AC
authors.lastname: Carvalho
authors.forename: Fábrice Santana
authors.initials: FS
authors.lastname: Coelho
authors.forename: Neide Hiromi Tokumaru
authors.initials: NH
authors.lastname: Miyazaki
authors.forename: Elizabeth Andrade
authors.initials: EA
authors.lastname: Marques
authors.forename: Alberto
authors.initials: A
authors.lastname: Chebabo
authors.forename: Andréa D'Avila
authors.initials: AD
authors.lastname: Freitas
authors.forename: Otília
authors.initials: O
authors.lastname: Lupi
authors.forename: Rafael Silva
authors.initials: RS
authors.lastname: Duarte
mesh_terms: D000447:Aldehydes / Q000494:pharmacology*; D004202:Disinfectants / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial / Q000187:drug effects*; D005976:Glutaral / Q000008:administration & dosage / Q000494:pharmacology*; D006801:Humans; D008826:Microbial Sensitivity Tests; D009161:Mycobacterium / Q000145:classification / Q000187:drug effects* / Q000302:isolation & purification; D010463:Peracetic Acid / Q000494:pharmacology*; D011183:Postoperative Complications / Q000382:microbiology
chemical_list: D000447:Aldehydes; D004202:Disinfectants; D010463:Peracetic Acid; D005976:Glutaral