Exposure to tebuconazol in rice field and laboratory conditions induces oxidative stress in carp (Cyprinus carpio).
Pesticides can have an effect on the biochemical and physiological functions of living organisms. The changes seen in fish and their response to pesticides can be used as an example for vertebrate toxicity. In this study, carp fish (Cyprinus carpio) were exposed to different concentrations of tebuconazol fungicide, by rice field (31.95 μg/L) and laboratory (33.47 and 36.23 μg/L) conditional testing, during a 7 day period. Parameters such thiobarbituric acid-reactive substance levels (TBARS), protein carbonyl, catalase, glutathione S-transferase and acetylcholinesterase activities were studied, using the liver, brain and white muscle of the fish. The field experiment showed that the TBARS levels were increased in all the analyzed tissues. Similarly, the protein carbonyl of the liver and the brain AChE activity increased after 7 days. The laboratory experiment demonstrated that the TBARS levels in the liver were increased in both of the concentration tests. TBARS levels in the muscle increased only by the lowest test concentration. On the other hand, the protein carbonyl was increased only by the highest concentration. The results indicate that the tebuconazol exposure from the field and laboratory conditions directly affected the health of the fish, showing the occurrence of oxidative stress.
Toni
Cândida
C
Adaptive Laboratory of Biochemistry, Post-Graduation Program in Biological Science, Toxicologic Biochemistry, Department of Chemistry, University of Santa Maria (UFSM), Santa Maria, Rio Grande do Sul, Brazil.
Loro
Vania Lucia
VL
Santi
Adriana
A
de Menezes
Charlene Cavalheiro
CC
Cattaneo
Roberta
R
Clasen
Bárbara Estevão
BE
Zanella
Renato
R
D000110:Acetylcholinesterase / Q000378:metabolism; D000383:Agriculture; D000818:Animals; D002347:Carps / Q000378:metabolism / Q000502:physiology*; D002374:Catalase / Q000378:metabolism; D018556:Crops, Agricultural; D005659:Fungicides, Industrial / Q000633:toxicity*; D005982:Glutathione Transferase / Q000378:metabolism; D012275:Oryza; D018384:Oxidative Stress; D014230:Triazoles / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000633:toxicity
D005659:Fungicides, Industrial; D014230:Triazoles; D014874:Water Pollutants, Chemical; C087114:tebuconazole; D002374:Catalase; D005982:Glutathione Transferase; D000110:Acetylcholinesterase