title: In vivo magnetic resonance imaging and optical imaging comparison of viable and nonviable mesenchymal stem cells with a bifunctional label.
abstract: The purpose of this study was to compare viable and nonviable bilabeled mesenchymal stem cells (MSCs) in arthritic joints with magnetic resonance imaging (MRI) and optical imaging (OI). MSCs were labeled with ferucarbotran and DiD. MRI and OI of bilabeled cells were compared with controls. Six rats with arthritis received intra-articular injections of bilabeled viable MSCs into the right knee and nonviable MSCs into the left knee. Animals underwent MRI and OI preinjection and at 4, 24, 48, and 72 hours postinjection. The results were analyzed with a mixed random effects model and Fisher probability. Bilabeled MSCs showed increased MRI and OI signals compared to unlabeled controls (p < .0001). After intra-articular injection, bilabeled MSCs caused significant T2 and T2* effect on MRI and fluorescence on OI up to 72 hours postinjection (p < .05). There was no significant difference between viable and nonviable MSC signal in the knee joints; however, some of the viable cells migrated to an adjacent inflamed ankle joint (p < .05). Immunohistochemistry confirmed viable MSCs in right knee and ankle joints and nonviable MSCs in the left knee. Viable and nonviable cells could not be differentiated with MRI or OI signal intensity but were differentiated based on their ability to migrate in vivo.
authors.affiliation: Department of Radiology, Mount Auburn Hospital, 330 Mount Auburn Street, Cambridge, MA 02138, USA. esutton@mah.harvard.edu
authors.forename: Elizabeth Jane
authors.initials: EJ
authors.lastname: Sutton
authors.forename: Tobias D
authors.initials: TD
authors.lastname: Henning
authors.forename: Sophie
authors.initials: S
authors.lastname: Boddington
authors.forename: Stavros
authors.initials: S
authors.lastname: Demos
authors.forename: Christian
authors.initials: C
authors.lastname: Krug
authors.forename: Reinhardt
authors.initials: R
authors.lastname: Meier
authors.forename: John
authors.initials: J
authors.lastname: Kornak
authors.forename: Shoujun
authors.initials: S
authors.lastname: Zhao
authors.forename: Rick
authors.initials: R
authors.lastname: Baehner
authors.forename: Sheida
authors.initials: S
authors.lastname: Sharifi
authors.forename: Heike
authors.initials: H
authors.lastname: Daldrup-Link
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001168:Arthritis / Q000628:therapy; D005260:Female; D008279:Magnetic Resonance Imaging / Q000379:methods*; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D016685:Mitomycin / Q000494:pharmacology; D051381:Rats
chemical_list: D016685:Mitomycin