title: Total chemical synthesis and X-ray structure of kaliotoxin by racemic protein crystallography.
abstract: Here we report the total synthesis of kaliotoxin by 'one pot' native chemical ligation of three synthetic peptides. A racemic mixture of D- and L-kaliotoxin synthetic protein molecules gave crystals in the centrosymmetric space group P1 that diffracted to atomic-resolution (0.95 Å), enabling the X-ray structure of kaliotoxin to be determined by direct methods.
authors.affiliation: Department of Chemistry, Institute for Biophysical Dynamics, The University of Chicago, Chicago, Illinois 60637, USA.
authors.forename: Brad L
authors.initials: BL
authors.lastname: Pentelute
authors.forename: Kalyaneswar
authors.initials: K
authors.lastname: Mandal
authors.forename: Zachary P
authors.initials: ZP
authors.lastname: Gates
authors.forename: Michael R
authors.initials: MR
authors.lastname: Sawaya
authors.forename: Todd O
authors.initials: TO
authors.lastname: Yeates
authors.forename: Stephen B H
authors.initials: SB
authors.lastname: Kent
mesh_terms: D000595:Amino Acid Sequence; D018360:Crystallography, X-Ray; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry; D017434:Protein Structure, Tertiary; D012604:Scorpion Venoms / Q000138:chemical synthesis* / Q000737:chemistry; D013237:Stereoisomerism
chemical_list: D010455:Peptides; D012604:Scorpion Venoms; C072276:kaliotoxin