title: Plasmid-mediated quinolone resistance gene detected in Escherichia coli from cattle.
abstract: Fluoroquinolones resistance in bacteria can be due to chromosomal and plasmid-mediated mechanisms. Of growing concern is the acquisition of genes encoding quinolone resistance in combination with other resistance mechanisms such as extended-spectrum beta-lactamases. In this study we describe the identification of an isolate of Escherichia coli from cattle which carried qnrS1 in combination with a blaCTX-M gene, although they were not co-localised on the same plasmid. In addition, using a DNA array it was possible to identify several other antimicrobial resistance genes in this isolate. This is the first report of a qnr gene in E. coli from cattle in the UK and highlights the need for surveillance of these emerging resistance mechanisms.
authors.affiliation: Veterinary Laboratories Agency, Woodham, Lane, New Haw, Addlestone, Surrey KT15 3NB, United Kingdom. m.kirchner@vla.defra.gsi.gov.uk
authors.forename: Miranda
authors.initials: M
authors.lastname: Kirchner
authors.forename: Heather
authors.initials: H
authors.lastname: Wearing
authors.forename: Christopher
authors.initials: C
authors.lastname: Teale
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002417:Cattle / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D029968:Escherichia coli Proteins / Q000235:genetics*; D005798:Genes, Bacterial; D020411:Oligonucleotide Array Sequence Analysis; D010957:Plasmids / Q000235:genetics*; D015363:Quinolones / Q000494:pharmacology; D006113:United Kingdom; D001618:beta-Lactamases / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; C485384:Qnr protein, E coli; D015363:Quinolones; D001618:beta-Lactamases