Epigenetic alteration of the Wnt inhibitory factor-1 promoter is common and occurs in advanced stage of Tunisian nasopharyngeal carcinoma.
Activation of the wingless-type (Wnt) signaling pathway is common in cancers. The Wnt inhibitory factor-1 (WIF-1) is a secreted antagonist that acts by binding to Wnt ligands. We examined by methylation-specific PCR (MSP), whether WIF-1 is inactivated in 68 nasopharyngeal carcinomas (NPC), and 10 normal mucosa. We showed that the WIF-1 promoter was methylated in 89.7% of tumors, whereas all normal mucosa were unmethylated. The WIF-1 methylation was associated with the tumor, node, and metastasis (TNM) (p = .003) and the age (p = .014). The Wnt-5a mRNA was higher in tumors and correlated with TNM (p = .012). The methylation of WIF-1 contributes to the activation of the Wnt pathway in NPC.
Fendri
Ali
A
Centre de Biotechnologie de Sfax, Tunisia.
Khabir
Abdelmajid
A
Hadri-Guiga
Boutheina
B
Sellami-Boudawara
Tahia
T
Daoud
Jamel
J
Frikha
Mounir
M
Ghorbel
Abdelmonem
A
Gargouri
Ali
A
Mokdad-Gargouri
Raja
R
D024506:5' Flanking Region / Q000235:genetics; D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics*; D000328:Adult; D001483:Base Sequence; D019175:DNA Methylation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D015999:Multivariate Analysis; D009297:Nasal Mucosa / Q000378:metabolism; D009303:Nasopharyngeal Neoplasms / Q000235:genetics* / Q000473:pathology; D009305:Nasopharynx / Q000378:metabolism; D009367:Neoplasm Staging; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011518:Proto-Oncogene Proteins / Q000235:genetics; D012097:Repressor Proteins / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014416:Tunisia; D051153:Wnt Proteins / Q000235:genetics; D000071818:Wnt-5a Protein; D055815:Young Adult
D048868:Adaptor Proteins, Signal Transducing; D011518:Proto-Oncogene Proteins; D012097:Repressor Proteins; C485569:WIF1 protein, human; C085389:WNT5A protein, human; D051153:Wnt Proteins; D000071818:Wnt-5a Protein