Fluorescent nanoparticles for observing primo vascular system along sciatic nerve.
The primo vascular system was found in the epineurium along the rat sciatic nerve following subcutaneous injection of fluorescent nanoparticles at the Zusanli acupoint (ST-36). Nanoparticles were injected into the primo-vessel near ST-36 and flowed along the sciatic nerve. Fluorescence revealed a structure in the epineurium that was hardly detectable. Images of the isolated sample stained with 4',6-diamidino-2-phenylindole were captured using confocal microscopy. These images showed the distinctive nuclei distribution and multi-lumen structure of primo-vessels that differentiate them from lymphatic vessels, blood capillaries and nerves. This study demonstrates a new use for nanoparticles in fluorescence reflectance imaging techniques during in vivo imaging of primo-vessels.
Jia
Zhao-Feng
ZF
Biomedical Physics Laboratory, Department of Physics and Astronomy, Seoul National University, 599 Gwanak, Gwanak-gu, Seoul, Korea.
Lee
Byung-Cheon
BC
Eom
Ki-Hoon
KH
Cha
Jin-Myung
JM
Lee
Jin-Kyu
JK
Su
Zhen-Dong
ZD
Yu
Wen-Hui
WH
Ryu
Pan Dong
PD
Soh
Kwang-Sup
KS
D000818:Animals; D005260:Female; D005456:Fluorescent Dyes / Q000032:analysis; D008297:Male; D016740:Meridians; D018613:Microscopy, Confocal; D053758:Nanoparticles / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D012584:Sciatic Nerve / Q000737:chemistry*; D013194:Staining and Labeling
D005456:Fluorescent Dyes