Naïve and memory B cells in the rhesus macaque can be differentiated by surface expression of CD27 and have differential responses to CD40 ligation.
The rhesus macaque (RM) model has the potential to be an invaluable tool for studying B cell populations during pathogenic infections, however, to date, there has been no definitive delineation of naïve and memory B cell populations in the RM. This has precluded a rigorous analysis of the generation, persistence and resolution of a pathogen-specific memory B cell response. The present study utilized multiple analyses to demonstrate that CD27 expression on B cells is consistent with a memory phenotype. Compared to CD20+CD27- B cells, CD20+CD27+ B cells were larger in size, and preferentially accumulated at effector sites. Direct sequence analysis revealed that CD20+CD27+ B cells had an increased frequency of point mutations that were consistent with somatic hypermutation and at a functional level, CD40 ligation improved CD20+CD27- but not CD20+CD27+ B cell survival in vitro. These data provide definitive evidence that the naïve and memory B cell populations of the RM can be differentiated using surface expression of CD27.
Kuhrt
David
D
Department of Immunology, University of Pittsburgh, Pittsburgh PA 15261, USA.
Faith
Seth
S
Hattemer
Angela
A
Leone
Amanda
A
Sodora
Donald
D
Picker
Louis
L
Borghesi
Lisa
L
Cole
Kelly Stefano
KS
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology*; D001483:Base Sequence; D015415:Biomarkers / Q000097:blood; D019013:CD40 Antigens / Q000276:immunology; D023201:CD40 Ligand / Q000276:immunology*; D005434:Flow Cytometry / Q000662:veterinary; D007156:Immunologic Memory / Q000276:immunology*; D016130:Immunophenotyping / Q000662:veterinary; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000276:immunology; D008253:Macaca mulatta / Q000097:blood / Q000276:immunology*; D008969:Molecular Sequence Data; D012313:RNA / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D027041:Somatic Hypermutation, Immunoglobulin / Q000276:immunology; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7 / Q000096:biosynthesis* / Q000097:blood / Q000276:immunology
D015415:Biomarkers; D019013:CD40 Antigens; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7; D023201:CD40 Ligand; D012313:RNA