Effect of hydrophobic/hydrophilic characteristics of magnetic microspheres on the immobilization of BSA.
Core-shell magnetic poly(styrene-acrylamide-acrylic acid) microspheres with carboxyl groups were successfully synthesized via dispersion copolymerization in the presence of nano-particle of Fe(3)O(4). The microspheres were characterized by FTIR spectra. They were used as carrier to immobilize bovine serum albumin (BSA). To investigate the effect of the microsphere surface properties on the immobilization of BSA, a series of microspheres with different hydrophobic/hydrophilic surface characteristics were prepared by adjusting molar percentages of monomers. The results showed that microspheres with different hydrophobicities/hydrophilicities had different immobilized ratios of BSA. In comparison with microspheres having hydrophilic characteristics those with hydrophobic characteristics had a much higher immobilized ratio. The possible reasons for these observations are discussed. In addition, ester activation and coupling times were optimized with respect to immobilized ratio.
Zhang
Dong-Hao
DH
College of Pharmacy, Hebei University, Baoding 071002, China. zhangdonghao1@gmail.com
Zhang
Ya-Fang
YF
Zhi
Gao-Ying
GY
Xie
Yu-Lei
YL
D000179:Acrylates / Q000737:chemistry; D000818:Animals; D002417:Cattle; D004952:Esters; D052203:Ferrosoferric Oxide / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D008280:Magnetics; D046529:Microscopy, Electron, Transmission / Q000379:methods; D008863:Microspheres; D011108:Polymers / Q000737:chemistry; D011137:Polystyrenes / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D013499:Surface Properties
D000179:Acrylates; D004952:Esters; D011108:Polymers; D011137:Polystyrenes; D012710:Serum Albumin, Bovine; C036658:acrylic acid; D052203:Ferrosoferric Oxide