title: Sequencing of multiple clostridial genomes related to biomass conversion and biofuel production.
abstract: Modern methods to develop microbe-based biomass conversion processes require a system-level understanding of the microbes involved. Clostridium species have long been recognized as ideal candidates for processes involving biomass conversion and production of various biofuels and other industrial products. To expand the knowledge base for clostridial species relevant to current biofuel production efforts, we have sequenced the genomes of 20 species spanning multiple genera. The majority of species sequenced fall within the class III cellulosome-encoding Clostridium and the class V saccharolytic Thermoanaerobacteraceae. Species were chosen based on representation in the experimental literature as model organisms, ability to degrade cellulosic biomass either by free enzymes or by cellulosomes, ability to rapidly ferment hexose and pentose sugars to ethanol, and ability to ferment synthesis gas to ethanol. The sequenced strains significantly increase the number of noncommensal/nonpathogenic clostridial species and provide a key foundation for future studies of biomass conversion, cellulosome composition, and clostridial systems biology.
authors.affiliation: Department of Botany and Microbiology, University of Oklahoma, Norman, Oklahoma 73019, USA.
authors.forename: Christopher L
authors.initials: CL
authors.lastname: Hemme
authors.forename: Housna
authors.initials: H
authors.lastname: Mouttaki
authors.forename: Yong-Jin
authors.initials: YJ
authors.lastname: Lee
authors.forename: Gengxin
authors.initials: G
authors.lastname: Zhang
authors.forename: Lynne
authors.initials: L
authors.lastname: Goodwin
authors.forename: Susan
authors.initials: S
authors.lastname: Lucas
authors.forename: Alex
authors.initials: A
authors.lastname: Copeland
authors.forename: Alla
authors.initials: A
authors.lastname: Lapidus
authors.forename: Tijana
authors.initials: T
authors.lastname: Glavina del Rio
authors.forename: Hope
authors.initials: H
authors.lastname: Tice
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Saunders
authors.forename: Thomas
authors.initials: T
authors.lastname: Brettin
authors.forename: John C
authors.initials: JC
authors.lastname: Detter
authors.forename: Cliff S
authors.initials: CS
authors.lastname: Han
authors.forename: Sam
authors.initials: S
authors.lastname: Pitluck
authors.forename: Miriam L
authors.initials: ML
authors.lastname: Land
authors.forename: Loren J
authors.initials: LJ
authors.lastname: Hauser
authors.forename: Nikos
authors.initials: N
authors.lastname: Kyrpides
authors.forename: Natalia
authors.initials: N
authors.lastname: Mikhailova
authors.forename: Zhili
authors.initials: Z
authors.lastname: He
authors.forename: Liyou
authors.initials: L
authors.lastname: Wu
authors.forename: Joy D
authors.initials: JD
authors.lastname: Van Nostrand
authors.forename: Bernard
authors.initials: B
authors.lastname: Henrissat
authors.forename: Qiang
authors.initials: Q
authors.lastname: He
authors.forename: Paul A
authors.initials: PA
authors.lastname: Lawson
authors.forename: Ralph S
authors.initials: RS
authors.lastname: Tanner
authors.forename: Lee R
authors.initials: LR
authors.lastname: Lynd
authors.forename: Juergen
authors.initials: J
authors.lastname: Wiegel
authors.forename: Matthew W
authors.initials: MW
authors.lastname: Fields
authors.forename: Adam P
authors.initials: AP
authors.lastname: Arkin
authors.forename: Christopher W
authors.initials: CW
authors.lastname: Schadt
authors.forename: Bradley S
authors.initials: BS
authors.lastname: Stevenson
authors.forename: Michael J
authors.initials: MJ
authors.lastname: McInerney
authors.forename: Yunfeng
authors.initials: Y
authors.lastname: Yang
authors.forename: Hailiang
authors.initials: H
authors.lastname: Dong
authors.forename: Defeng
authors.initials: D
authors.lastname: Xing
authors.forename: Nanqi
authors.initials: N
authors.lastname: Ren
authors.forename: Aijie
authors.initials: A
authors.lastname: Wang
authors.forename: Raymond L
authors.initials: RL
authors.lastname: Huhnke
authors.forename: Jonathan R
authors.initials: JR
authors.lastname: Mielenz
authors.forename: Shi-You
authors.initials: SY
authors.lastname: Ding
authors.forename: Michael E
authors.initials: ME
authors.lastname: Himmel
authors.forename: Safiyh
authors.initials: S
authors.lastname: Taghavi
authors.forename: Daniël
authors.initials: D
authors.lastname: van der Lelie
authors.forename: Edward M
authors.initials: EM
authors.lastname: Rubin
authors.forename: Jizhong
authors.initials: J
authors.lastname: Zhou
mesh_terms: D056804:Biofuels; D018533:Biomass; D003013:Clostridium / Q000235:genetics* / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D016680:Genome, Bacterial; D008969:Molecular Sequence Data; D046968:Thermoanaerobacter / Q000235:genetics*
chemical_list: D056804:Biofuels