title: Fluorescent nanoparticles for observing primo vascular system along sciatic nerve.
abstract: The primo vascular system was found in the epineurium along the rat sciatic nerve following subcutaneous injection of fluorescent nanoparticles at the Zusanli acupoint (ST-36). Nanoparticles were injected into the primo-vessel near ST-36 and flowed along the sciatic nerve. Fluorescence revealed a structure in the epineurium that was hardly detectable. Images of the isolated sample stained with 4',6-diamidino-2-phenylindole were captured using confocal microscopy. These images showed the distinctive nuclei distribution and multi-lumen structure of primo-vessels that differentiate them from lymphatic vessels, blood capillaries and nerves. This study demonstrates a new use for nanoparticles in fluorescence reflectance imaging techniques during in vivo imaging of primo-vessels.
authors.affiliation: Biomedical Physics Laboratory, Department of Physics and Astronomy, Seoul National University, 599 Gwanak, Gwanak-gu, Seoul, Korea.
authors.forename: Zhao-Feng
authors.initials: ZF
authors.lastname: Jia
authors.forename: Byung-Cheon
authors.initials: BC
authors.lastname: Lee
authors.forename: Ki-Hoon
authors.initials: KH
authors.lastname: Eom
authors.forename: Jin-Myung
authors.initials: JM
authors.lastname: Cha
authors.forename: Jin-Kyu
authors.initials: JK
authors.lastname: Lee
authors.forename: Zhen-Dong
authors.initials: ZD
authors.lastname: Su
authors.forename: Wen-Hui
authors.initials: WH
authors.lastname: Yu
authors.forename: Pan Dong
authors.initials: PD
authors.lastname: Ryu
authors.forename: Kwang-Sup
authors.initials: KS
authors.lastname: Soh
mesh_terms: D000818:Animals; D005260:Female; D005456:Fluorescent Dyes / Q000032:analysis; D008297:Male; D016740:Meridians; D018613:Microscopy, Confocal; D053758:Nanoparticles / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D012584:Sciatic Nerve / Q000737:chemistry*; D013194:Staining and Labeling
chemical_list: D005456:Fluorescent Dyes