title: Simultaneous extraction of carotenoids and transfructosylating enzyme from Xanthophyllomyces dendrorhous by a bead beater.
abstract: Conditions for the cell disruption of Xanthophyllomyces dendrorhous by using a bead beater have been optimized with respect to maximizing the recovery of carotenoids and transfructosylating enzymes in a one-step process. The optimal conditions for a one-step extraction of carotenoids and transfructosylating enzymes from Xanthophyllomyces dendrorhous were: 30 g dry cells/l being disintegrated with 0.5 mm diam. glass beads at 10°C for 15 min using a bead beater. More than 90% yeast disruption and, when extracted with hexane/ethyl acetate, 28.6 mg carotenoids/l in the organic solvent phase and 440 U transfructosylating enzyme activity/mg protein in the water phase were obtained.
authors.affiliation: Department of Food Science and Biotechnology, National Chung Hsing University, 250 Kuokuang Road, Taichung city, Taiwan, 40227, People's Republic of China.
authors.forename: Shu-Chih
authors.initials: SC
authors.lastname: Lee
authors.forename: Tony J
authors.initials: TJ
authors.lastname: Fang
mesh_terms: D001487:Basidiomycota / Q000737:chemistry* / Q000201:enzymology*; D001709:Biotechnology / Q000379:methods*; D002338:Carotenoids / Q000302:isolation & purification*; D006602:Hexosyltransferases / Q000302:isolation & purification*; D001426:Bacterial Proteins
chemical_list: D002338:Carotenoids; D006602:Hexosyltransferases; C021451:inulosucrase; D001426:Bacterial Proteins