title: Complex surface concentration gradients by stenciled "electro click chemistry".
abstract: Complex one- or two-dimensional concentration gradients of alkynated molecules are produced on azidized conducting polymer substrates by stenciled "electro click chemistry". The latter describes the local electrochemical generation of catalytically active Cu(I) required to complete a "click reaction" between alkynes and azides at room temperature. A stencil on the counter electrode defines the shape and multiplicity of the gradient(s) on the conducting polymer substrate, while the specific reaction conditions control gradient steepness and the maximum concentration deposited. Biologically active ligands including cell binding peptides are patterned in gradients by this method without losing their biological function or the conductivity of the polymer.
authors.affiliation: Department of Micro- and Nanotechnology, Technical University of Denmark, DTU Nanotech, Frederiksborgvej 399, 4000 Roskilde, Denmark.
authors.forename: Thomas S
authors.initials: TS
authors.lastname: Hansen
authors.forename: Johan U
authors.initials: JU
authors.lastname: Lind
authors.forename: Anders E
authors.initials: AE
authors.lastname: Daugaard
authors.forename: Søren
authors.initials: S
authors.lastname: Hvilsted
authors.forename: Thomas L
authors.initials: TL
authors.lastname: Andresen
authors.forename: Niels B
authors.initials: NB
authors.lastname: Larsen
mesh_terms: D000480:Alkynes / Q000737:chemistry; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000737:chemistry; D057930:Click Chemistry; D003300:Copper / Q000737:chemistry; D004553:Electric Conductivity; D004566:Electrodes; D055767:Immobilized Proteins / Q000737:chemistry; D009532:Nickel / Q000737:chemistry; D009571:Nitrilotriacetic Acid / Q000737:chemistry; D009842:Oligopeptides / Q000737:chemistry; D056951:Photoelectron Spectroscopy; D011108:Polymers / Q000737:chemistry; D013499:Surface Properties
chemical_list: D000480:Alkynes; D019086:Bridged Bicyclo Compounds, Heterocyclic; D055767:Immobilized Proteins; D009842:Oligopeptides; D011108:Polymers; C121383:poly(3,4-ethylene dioxythiophene); D003300:Copper; C047981:arginyl-glycyl-aspartic acid; D009532:Nickel; D009571:Nitrilotriacetic Acid