Insects as model systems in cell biology.
For almost 100 years, insects have been favorable "model systems" in biology. Just to mention a few examples: fruit flies in genetics and developmental biology; bugs and caterpillars in hormone research; houseflies, blowflies, and locusts in neurobiology; silk moths in pheromone research; honeybees and crickets in neuroethology. For more than 50 years the electron microscope (EM) has been a valuable tool in analyzing the structure of cells and organs of these creatures. However, progress in specimen preparation was relatively slow compared with mammalian material and, in 1970, it was taken for granted that insects were much more difficult to fix than mammals. Since then, methods have dramatically improved, and satisfactory results can now be obtained routinely with chemical as well as cryofixation. In this chapter we briefly demonstrate what can be achieved with insect material, and help the researcher to find the most appropriate method for her/his systems and scientific questions.
Keil
Thomas A
TA
Department Molecular Structural Biology, Max-Planck-Institute for Biochemistry, D-82152 Martinsried, Germany.
Steinbrecht
R Alexander
RA
D000818:Animals; D016477:Artifacts; D003585:Cell Biology; D015925:Cryopreservation / Q000295:instrumentation / Q000379:methods; D017110:Freeze Substitution / Q000379:methods; D007150:Immunohistochemistry / Q000379:methods; D007313:Insecta / Q000648:ultrastructure*; D008854:Microscopy, Electron / Q000295:instrumentation / Q000379:methods; D008954:Models, Biological; D016707:Tissue Fixation / Q000379:methods*