title: Antibiotic-resistant Pseudomonas aeruginosa from hospital wastewater and superficial water: are they genetically related?
abstract: Many studies have reported the presence of antibiotic-resistant Pseudomonas aeruginosa in environmental samples such as hospital wastewater and surface water. The present study evaluated the contribution of untreated hospital wastewater to the dissemination of resistant P. aeruginosa strains in aquatic environments, through the analysis of their antibiotic susceptibility profile and genetic similarity. Wastewater samples were collected from two hospitals located in Rio Grande do Sul, RS, Brazil. Superficial water samples were collected from water bodies that received this wastewater discharge. The antibiotic susceptibility profiles of the strains were determined using the disk-diffusion technique and their genotyping was done by amplification of the Enterobacterial Repetitive Intergenic Consensus sequences (ERIC-PCR). The antibiotic resistance was higher among the hospital wastewater strains and the multiresistant phenotype was also observed only among these strains. The ERIC-PCR profiles did not reveal any genetic similarity among the P. aeruginosa strains from the wastewater and superficial water samples. On the contrary, they showed that genetically distinct populations were established in these different environments and probably that some other contamination source could be contributing to the presence of resistant strains in these water bodies.
authors.affiliation: Departamento de Microbiologia, Imunologia e Parasitologia, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Rua Sarmento Leite, 500 Cidade Baixa, Porto Alegre, RS, CEP 90050-170, Brazil.
authors.forename: Daiane Bopp
authors.initials: DB
authors.lastname: Fuentefria
authors.forename: Alessandra Einsfeld
authors.initials: AE
authors.lastname: Ferreira
authors.forename: Gertrudes
authors.initials: G
authors.lastname: Corção
mesh_terms: D001938:Brazil; D052978:Disk Diffusion Antimicrobial Tests; D004352:Drug Resistance, Microbial; D005838:Genotype; D006761:Hospitals; D008506:Medical Waste; D016133:Polymerase Chain Reaction; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D014865:Waste Disposal, Fluid
chemical_list: D008506:Medical Waste