title: Identification of four novel KIR2DL2 alleles and two novel KIR2DL3 alleles in an East African population.
abstract: We report four novel KIR2DL2 alleles and two novel KIR2DL3 alleles identified from an East African population using sequence-based typing. Sequencing and molecular cloning of exon 4 confirmed that the new 2DL2 alleles were identical to 2DL2*003, except for the following nucleotide differences: 2DL2*00601 had a difference at codon 16 (CGC→CCC), resulting in a coding change from arginine to proline; 2DL2*00602 also had this difference at codon 16, as well as a synonymous difference (GAT→GAC) at codon 31; 2DL2*00303 had a synonymous difference (AAA→AAG) at codon 61; and 2DL2*00304 had a synonymous difference (GGG→GGA) at codon 75. 2DL3*017 was identical to 2DL3*005 except at codon 11 (CGG→CTG, arginine→leucine) and exon 9, codons 297 (CAC→CGC, histidine→arginine) and 321 (TGA→AGA, stop codon→arginine). 2DL3*00104 was identical to KIR2DL3*001 except for a synonymous difference (GAG→GAA) at codon 54. Identification of novel killer cell immunoglobulin-like receptor (KIR) alleles is a testament to the genetic diversity in this population.
authors.affiliation: Department of Medical Microbiology, University of Manitoba, Winnipeg, Manitoba, Canada.
authors.forename: Rae-Anne
authors.initials: RA
authors.lastname: Hardie
authors.forename: Chris
authors.initials: C
authors.lastname: Czarnecki
authors.forename: T Blake
authors.initials: TB
authors.lastname: Ball
authors.forename: Francis A
authors.initials: FA
authors.lastname: Plummer
authors.forename: Ma
authors.initials: M
authors.lastname: Luo
mesh_terms: D000351:Africa, Eastern; D000483:Alleles; D044383:Black People / Q000235:genetics*; D003001:Cloning, Molecular; D003062:Codon / Q000235:genetics; D005091:Exons / Q000235:genetics; D005260:Female; D014644:Genetic Variation; D006801:Humans; D054343:Receptors, KIR2DL2 / Q000235:genetics*; D054344:Receptors, KIR2DL3 / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D003062:Codon; C515077:KIR2DL2 protein, human; C515078:KIR2DL3 protein, human; D054343:Receptors, KIR2DL2; D054344:Receptors, KIR2DL3