Using the residue of spirit production and bio-ethanol for protein production by yeasts.
The residue (vinasse) formed during the distillation of bio-ethanol and cachaça, a traditional rum-type spirit produced from sugar-cane in Brazil, is highly harmful if discharged into the environment due to high values of COD and BOD. One possibility for minimizing the impact of vinasse in soils and waters is to use the residue in the production of microbial biomass for use as an animal feed supplement that will provide high levels on nitrogen (>9% d.m.) and low content of nucleic (≤ 10% d.m.) This paper reports the production and quality of biomass produced from fermentation of Saccharomyces cerevisiae and Candida parapsilosis in culture media under 12 different culture conditions and the respective effects of each variable (glucose, yeast extract, peptone, potassium phosphate, vinasse, pH and temperature). Of the S. cerevisiae isolates tested, two (VR1 and PE2) originating from fuel alcohol-producing plants were identified as offering the best potential for the industrial production of single cell protein from vinasse due to highest biomass productivity. Our results showed a potential viable and economic use of vinasse.
Silva
Cristina F
CF
Departamento de Biologia, Universidade Federal de Lavras, 37200-000 Lavras, MG, Brazil. cristinafsb@dbi.ufla.br
Arcuri
Silvio L
SL
Campos
Cássia R
CR
Vilela
Danielle M
DM
Alves
José G L F
JG
Schwan
Rosane F
RF
D000434:Alcoholic Beverages; D000821:Animal Feed; D002175:Candida / Q000254:growth & development / Q000378:metabolism / Q000648:ultrastructure; D004044:Dietary Proteins / Q000378:metabolism*; D057168:Distillation; D000431:Ethanol / Q000737:chemistry / Q000378:metabolism*; D005656:Fungal Proteins / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D009584:Nitrogen / Q000032:analysis / Q000378:metabolism; D015596:Nutrition Assessment; D012441:Saccharomyces cerevisiae / Q000254:growth & development / Q000378:metabolism / Q000648:ultrastructure; D013696:Temperature; D018505:Waste Management / Q000379:methods*; D015003:Yeasts / Q000378:metabolism*
D004044:Dietary Proteins; D005656:Fungal Proteins; C012551:single cell proteins; D000431:Ethanol; D009584:Nitrogen