[Effects of saline-alkali and drought stress on seed germination and TvNHX1 expression of Tripolium vulgare].
By applying 40-400 mmol x L(-1) NaCl, 20-200 mmol x L(-1) NaHCO3, and 5%-30% PEG, this paper studied the effects of saline-alkali and drought stress on the seed germination and Na+/H+ antiporter gene (TvNHX1) expression of Tripolium vulgare. 40-160 mmol x L(-1) NaCl, 20 mmol x L(-1) NaHCO3, and 5%-10% PEG-6000 had less effects on the seed germination; while > or = 240 mmol x L(-1) NaCl decreased the seed germination rate, root length, and shoot length (P < 0.05), > or = 50 mmol x L(-1) NaHCO3 decreased the seed germination rate (P < 0.05), 130 mmol x L(-1) NaHCO3 decreased the seed germination potential, root length, and shoot length (P < 0.05), and > or = 15% PEG delayed the seed germination. TvNHX1 transcripts were basically constitutive at germination stage, but their expression increased distinctly at 160 mmol x L(-1) NaCl, 100 mmol x L(-1) NaHCO3, and 10% PEG. Under salt and drought stress, the expression of TvNHX1 changed in-phase with the phenotype change of germinated seeds, suggesting that TvNHX1 play important roles in the tolerance of T. vulgare against adversity stress.
Wei
Jia-Li
JL
College of Life Sciences and Technology, Harbin Normal University, Harbin 150025, China. weijiali19830320@163.com
Cui
Ji-Zhe
JZ
Zhao
He-Xiang
HX
Mi
Xiao-Ju
XJ
D000468:Alkalies / Q000633:toxicity*; D019659:Asteraceae / Q000187:drug effects* / Q000235:genetics; D055864:Droughts; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D018525:Germination / Q000187:drug effects*; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics; D012965:Sodium Chloride / Q000633:toxicity*; D013312:Stress, Physiological / Q000502:physiology
D000468:Alkalies; D010940:Plant Proteins; D012965:Sodium Chloride