Development of a high-performance liquid chromatography method to monitor the residues of benzimidazoles in bovine milk.
A reversed-phase high-performance liquid chromatography with ultraviolet (UV) detection was developed that can determine 11 benzimidazole (BZDs) and 10 metabolites of albendazole, fenbendazole and mebendazole in bovine milk. Samples were extracted with acetonitrile and purified by mixed-mode cation exchange (MCX) solid phase extraction cartridges. LC separations were performed on a C(18) column with gradient elution using acetonitrile and ammonium acetate solution. The UV-detection was set at 292nm. The method is very sensitive to each analyte with limits of quantification (LOQs) of lower than 10μgkg(-1). The recoveries of the BZDs and their metabolites spiked in milk were more than 78% with between-day relative standard deviation values less than 16% at the concentration of 10, 50 and 100μgkg(-1). The method developed has been successfully applied to monitoring real samples containing BZDs, which demonstrated that it is a simple, fast and robust method, and could be used as a regulatory toll to determine the residues of BZDs in milk.
Chen
Dongmei
D
MAO Key Laboratory of Food Safety Evaluation/National Reference Laboratory of Veterinary Drug Residues (HZAU), Huazhong Agricultural University, Wuhan, Hubei 430070, PR China.
Tao
Yanfei
Y
Liu
Zhaoying
Z
Liu
Zhenli
Z
Huang
Lingli
L
Wang
Yulian
Y
Pan
Yuanhu
Y
Peng
Dapeng
D
Dai
Menghong
M
Yuan
Zonghui
Z
D000085:Acetates; D000097:Acetonitriles; D000818:Animals; D000871:Anthelmintics / Q000032:analysis; D001562:Benzimidazoles / Q000032:analysis* / Q000737:chemistry; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008892:Milk / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000085:Acetates; D000097:Acetonitriles; D000871:Anthelmintics; D001562:Benzimidazoles; C018824:ammonium acetate; C032159:acetonitrile