Imaging fibroblast cells using atomic force microscopy (AFM).
Atomic force microscopy (AFM) can be used to visualize the three major cytoskeletal components that contribute to the mechanical properties of the cell. These are actin microfilaments, intermediate filaments, and microtubules. In this protocol, rat embryonic fibroblasts expressing actin tagged with green fluorescent protein (GFP) are used to demonstrate this procedure.
Plodinec
Marija
M
Loparic
Marko
M
Aebi
Ueli
U
D000818:Animals; D005347:Fibroblasts / Q000166:cytology*; D018625:Microscopy, Atomic Force; D057054:Molecular Imaging / Q000379:methods*; D051381:Rats