title: The effects of human umbilical cord blood transplantation in rats with experimentally induced spinal cord injury.
abstract: OBJECTIVE
  Even though there have been many efforts to recover neuronal dysfunction following spinal cord injuries, there are limitations to the treatment of these injuries. The purpose of this laboratory investigation was to determine the clinical and neurophysiological effects of human umbilical cord blood (HUCB) transplantation in a rat hemisection model of spinal cord injury.
  
  
  METHODS
  In this study, experimental hemisection of the thoracic spinal cord was performed in rats. The rats were divided into 4 groups (6 rats in each group). One group of rats (Group 1) underwent thoracic laminectomy only. Rats in Group 2 underwent laminectomy and right hemisection of the thoracic spinal cord. Rats in Group 3 underwent right hemisection and implantation of freshly obtained HUCB on Day 0 postinjury. Rats in Group 4 underwent hemisection and implantation of freshly obtained HUCB on Day 4 postinjury. Clinical evaluations of rat motor function included the following: neurological examination, Rotarod performance, and inclined plane tests. Rats also underwent reflex evaluation.
  
  
  RESULTS
  The neurological examinations revealed that the frequency of plegic rats was 70.8% at the beginning of the study across all 4 groups; this value decreased to 20.8% by the end of the study. The percentage of rats with a normal examination increased from 25% to 50%. The results of Rotarod performance and 8-week inclined plane performance tests showed statistical significance (p < 0.05) in an overall group comparison across all time points. At the end of the 8 weeks, a statistically significant difference was found in the inclined plane test results between rats in Groups 1 and 2. There were no statistically significant differences between Groups 1, 3, and 4 (p < 0.05). When the reflex responses of the hemisectioned sides were compared, statistically significant differences were detected between groups (p < 0.05). All groups were significantly different with regard to the right-side reflex response score (p < 0.05). Spinal cord preparations of rats in all groups were examined for histopathological changes.
  
  
  CONCLUSIONS
  Human umbilical cord blood is stem cell rich and easily available, and it carries less risk of inducing a graft-versus-host reaction in the recipient. Human umbilical cord blood serum is also noted to contain stem cell–promoting factors, which is why cell isolation was not used in this study. Freshly obtained cord blood was also used because storage of cord blood has been reported to have some negative effects on stem cells. Transplantation of freshly obtained HUCB into the hemisectioned spinal cord experimental model demonstrated clinical and neurophysiological improvement.
authors.affiliation: Department of Neurosurgery, Pendik State Hospital, Istanbul, Turkey.
authors.forename: Tuncay
authors.initials: T
authors.lastname: Kaner
authors.forename: Tolga
authors.initials: T
authors.lastname: Karadag
authors.forename: Bayram
authors.initials: B
authors.lastname: Cirak
authors.forename: Haydar Ali
authors.initials: HA
authors.lastname: Erken
authors.forename: Aysun
authors.initials: A
authors.lastname: Karabulut
authors.forename: Yilmaz
authors.initials: Y
authors.lastname: Kiroglu
authors.forename: Semih
authors.initials: S
authors.lastname: Akkaya
authors.forename: Feridun
authors.initials: F
authors.lastname: Acar
authors.forename: Erdal
authors.initials: E
authors.lastname: Coskun
authors.forename: Osman
authors.initials: O
authors.lastname: Genc
authors.forename: Nagihan
authors.initials: N
authors.lastname: Colakoglu
mesh_terms: D000818:Animals; D005260:Female; D005312:Fetal Blood / Q000637:transplantation*; D006801:Humans; D009043:Motor Activity; D009460:Neurologic Examination; D051381:Rats; D017208:Rats, Wistar; D012018:Reflex; D044968:Regenerative Medicine / Q000379:methods; D012584:Sciatic Nerve / Q000503:physiopathology; D013116:Spinal Cord / Q000503:physiopathology; D013119:Spinal Cord Injuries / Q000503:physiopathology* / Q000628:therapy*; D013126:Spinal Nerve Roots / Q000503:physiopathology; D013904:Thoracic Vertebrae