Development of a porcine epidemic diarrhea virus M protein-based ELISA for virus detection.
A membrane (M), protein-based ELISA was developed to detect porcine epidemic diarrhea virus (PEDV). The M gene of PEDV was expressed in Escherichia coli. The purified recombinant M protein was used to immunize rabbits to generate a polyclonal antibody. Immunofluorescence analysis indicated that the anti-PEDV-M antibody reacted with PEDV-infected cells. The antibody was utilized to develop an indirect ELISA to detect PEDV. Other viruses, porcine transmissible gastroenteritis coronavirus, avian infectious bronchitis coronavirus, porcine reproductive and respiratory syndrome virus, classic swine fever virus and porcine pseudorabies virus, were unreactive.
Ren
Xiaofeng
X
Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai Street, Xiangfang District, Harbin 150030, China. rxfemail@yahoo.com.cn
Suo
Siqingaowa
S
Jang
Yong-Suk
YS
D000818:Animals; D000914:Antibodies, Viral / Q000302:isolation & purification; D018352:Coronavirus Infections / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D000086862:Coronavirus M Proteins; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D053485:Porcine epidemic diarrhea virus / Q000276:immunology / Q000302:isolation & purification*; D011817:Rabbits; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D012680:Sensitivity and Specificity; D013553:Swine Diseases / Q000175:diagnosis* / Q000821:virology; D014730:Veterinary Medicine / Q000379:methods*; D014763:Viral Matrix Proteins / Q000235:genetics / Q000276:immunology*; D014773:Virology / Q000379:methods*
D000914:Antibodies, Viral; D000086862:Coronavirus M Proteins; C000706236:M protein, Porcine epidemic diarrhea virus; D011994:Recombinant Proteins; D014763:Viral Matrix Proteins