Whole-mount in situ hybridization for analysis of gene expression during Aedes aegypti development.
Blood-feeding mosquitoes, including the dengue and yellow fever vector Aedes aegypti, transmit many of the world's deadliest diseases. Such diseases have resurged in developing countries and pose clear threats for epidemic outbreaks in developed countries. Recent mosquito genome projects have stimulated interest in the potential for arthropod-borne disease control by genetic manipulation of vector insects, and genes that regulate development are of particular interest. This protocol for whole-mount in situ hybridization can be used to analyze gene expression in Ae. aegypti embryos and larvae, a critical aspect of understanding developmental gene function in this vector mosquito.
Haugen
Morgan
M
Department of Medical and Molecular Genetics, Indiana University School of Medicine, South Bend, IN 46617, USA.
Tomchaney
Michael
M
Kast
Kristopher
K
Flannery
Ellen
E
Clemons
Anthony
A
Jacowski
Caitlin
C
Simanton Holland
Wendy
W
Le
Christy
C
Severson
David
D
Duman-Scheel
Molly
M
D000330:Aedes / Q000235:genetics* / Q000254:growth & development*; D000818:Animals; D046508:Culture Techniques / Q000379:methods; D018507:Gene Expression Regulation, Developmental; D017403:In Situ Hybridization / Q000379:methods*; D007303:Insect Vectors / Q000235:genetics* / Q000254:growth & development*