Evidence for a stem-cell lineage in corneal squamous cell carcinoma using synchrotron-based Fourier-transform infrared microspectroscopy and multivariate analysis.
The cornea is one of the few human tissues where the in situ locations of stem cells (SCs), transient-amplifying (TA) cells and terminally-differentiated (TD) cells have been relatively well localised and characterised. Mid-infrared (IR) (4000-400 cm(-1)) is absorbed by biological molecules and facilitates the acquisition in the biochemical-cell fingerprint region (1800-900 cm(-1)) of spectra representative of structure and function. Human cornea derived from normal or squamous cell carcinoma (SCC) samples were acquired, cryosectioned (10 µm), floated onto BaF(2) windows and interrogated using synchrotron-based radiation (SRS) Fourier-transform IR (FTIR) microspectroscopy. Spectra were analysed using principal component analysis (PCA) with or without linear discriminant analysis (LDA) to allow cluster analysis of the cell categories. A clear cell lineage emanating from SCs to TA cells to TD cells was noted in normal samples. Within the SCC samples, a small sub-population of the cell-derived spectra pointed to a SC-like phenotype with the vast majority pointing to a TA cell-like character; these cells would tend to be the most proliferative within a tissue. Our findings suggest that SRS FTIR microspectroscopy has the potential to identify and characterise cancer SCs.
Kelly
Jemma G
JG
Centre for Biophotonics, Lancaster Environment Centre, Lancaster University, Bailrigg. Lancaster, LA1 4YQ, UK.
Nakamura
Takahiro
T
Kinoshita
Shigeru
S
Fullwood
Nigel J
NJ
Martin
Francis L
FL
D015415:Biomarkers / Q000737:chemistry; D002294:Carcinoma, Squamous Cell / Q000737:chemistry* / Q000473:pathology; D019070:Cell Lineage; D016000:Cluster Analysis; D003316:Corneal Diseases / Q000473:pathology*; D005134:Eye Neoplasms / Q000737:chemistry* / Q000473:pathology; D006801:Humans; D015999:Multivariate Analysis; D025341:Principal Component Analysis; D017550:Spectroscopy, Fourier Transform Infrared / Q000295:instrumentation / Q000379:methods; D013234:Stem Cells / Q000737:chemistry* / Q000166:cytology; D017356:Synchrotrons
D015415:Biomarkers