[Screening of anti-diarrhea effective fractions from guava leaf].
OBJECTIVE
To screen the fractions from guava leaf to ascertain the effective anti-diarrhea fraction and to establish the method of quality control.


METHODS
A series of samples from guava leaf were prepared through extraction and separation by solvents progressively, and their effect on the mice diarrhea models induced by senna decoction were tested individually; and the constituent correlated with the effect of the samples on the models was determined by HPLC.


RESULTS
The effect of the fractions with moderate-polar glucosides from guava leaf was better than that of the other fractions; the effect of the fractions with the glucosides was correlated with the content of the quercetin glucosides in the fractions.


CONCLUSIONS
The fractions with the moderate-polar glucosides is the effective anti-diarrhea fraction of guava leaf; determination of the quercetin glucosides can be used for quality control of guava leaf and its extracts.
Lu
Wen-biao
WB
Guangzhou University of Chinese Medicine, Guangzhou 510405, China. vip.luwbl@gzhtcm.edu.cn
Zhang
Bei-en
BE
Wang
Jing
J
Lu
Rui-xin
RX
Li
Ru-liu
RL
Chen
Wei-wen
WW
D000818:Animals; D000930:Antidiarrheals / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D003967:Diarrhea / Q000139:chemically induced / Q000473:pathology*; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D005260:Female; D008297:Male; D051379:Mice; D018515:Plant Leaves / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry; D031647:Psidium / Q000737:chemistry*; D011786:Quality Control; D011794:Quercetin / Q000032:analysis* / Q000737:chemistry
D000930:Antidiarrheals; D004365:Drugs, Chinese Herbal; D011794:Quercetin