title: Induction, structural characterization, and genome sequence of Lv1, a prophage from a human vaginal Lactobacillus jensenii strain.
abstract: The prophage Lv1, harbored by a vaginal Lactobacillus jensenii isolate, was induced by several different anticancer, antimicrobial, and antiseptic agents, suggesting that they contribute to the adverse vaginal effects associated with their therapeutic use. Of special interest with respect to its novelty was the inducing effect of nonoxynol-9, a non-ionic detergent commonly used as a spermicide. The Lv1 genome consists of a 38,934-bp dsDNA molecule with cohesive ends, in which 48 ORFs were recognized, and is organized into functional modules. Lv1 belongs to the family Siphoviridae and, more precisely, to the proposed Sfi21-like genus. The capsid-tail junction of the Lv1 virions is fragile such that most particles become disrupted, suggesting that the virus is defective and thus unable to generate fertile progeny. However, genome analysis did not provide evidence of the defective nature of the prophage, other than the finding that its genome is shorter than those of other, related, phages. Further analysis indicated that prophage Lv1 suffered deletions in its right half to the extent that it no longer fulfill the minimum packaging limits, thereby generating the observed unstable particles.
authors.affiliation: Microbiology Unit, University Institute of Biotechnology, University of Oviedo, Oviedo, Spain.
authors.forename: Rebeca
authors.initials: R
authors.lastname: Martín
authors.forename: Susana
authors.initials: S
authors.lastname: Escobedo
authors.forename: Juan E
authors.initials: JE
authors.lastname: Suárez
mesh_terms: D000891:Anti-Infective Agents, Local / Q000378:metabolism; D000970:Antineoplastic Agents / Q000378:metabolism; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D023061:Gene Order; D016679:Genome, Viral; D006801:Humans; D007778:Lactobacillus / Q000302:isolation & purification / Q000821:virology*; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D017137:Nonoxynol / Q000378:metabolism; D016366:Open Reading Frames; D039002:Prophages / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D017422:Sequence Analysis, DNA; D017906:Siphoviridae / Q000302:isolation & purification / Q000648:ultrastructure; D013089:Spermatocidal Agents / Q000378:metabolism; D026801:Synteny; D014621:Vagina / Q000382:microbiology; D014764:Viral Proteins / Q000302:isolation & purification; D014771:Virion / Q000302:isolation & purification / Q000648:ultrastructure; D014775:Virus Activation / Q000187:drug effects*; D014779:Virus Replication / Q000187:drug effects
chemical_list: D000891:Anti-Infective Agents, Local; D000970:Antineoplastic Agents; D004279:DNA, Viral; D013089:Spermatocidal Agents; D014764:Viral Proteins; D017137:Nonoxynol