title: Potential role of bone marrow-derived cells in the turnover of mesothelium.
abstract: BACKGROUND
  Bone marrow cell has been proposed as a source of new mesothelium, but supporting evidence is rare. This study examines the validity of this hypothesis by using green fluorescent protein (GFP) and Y-chromosome trackers to identify the presence of donor marrow cells in peritoneal membrane of bone marrow transplant recipient mice.
  
  
  METHODS
  Cross-gender and GFP-mismatched bone marrow transplantation was undertaken in 20 FVB mice. Five recipients were killed 2, 4, and 6 weeks and 6 months later. Peritoneal tissues were obtained for the detection of GFP and Y chromosome by immunohistochemical staining (IHC) and chromogenic in situ hybridization (CISH).
  
  
  RESULTS
  GFP+ cells could be found in the peritoneal membrane of bone marrow transplant recipients. However, the level of engraftment was low, accounting for 0.9%, 0.8%, 0.7%, and 2.2% of the total counted mesothelial cells in intestinal serosa at 2, 4, and 6 weeks and 6 months post-transplantation, respectively. The presence of donor marrow cells within mesothelium was again confirmed by the detection of Y-chromosome-containing cells. Moreover, Y-chromosome+ cells incorporated within the mesothelium were positively stained by anticytokeratin antibody.
  
  
  CONCLUSIONS
  Donor marrow cells could attach to mesothelium and exhibit mesothelial marker cytokeratin in bone marrow transplant recipients. This finding suggests that bone marrow-derived cells might participate in the turnover of mesothelium.
authors.affiliation: Division of Nephrology, Chang Gung Memorial Hospital, Keelung, Taiwan. cksdavid@adm.cgmh.org.tw
authors.forename: Kuo-Su
authors.initials: KS
authors.lastname: Chen
authors.forename: Chao-Hung
authors.initials: CH
authors.lastname: Wang
authors.forename: Tzung-Hai
authors.initials: TH
authors.lastname: Yen
authors.forename: Jim-Ray
authors.initials: JR
authors.lastname: Chen
authors.forename: Ming-Jui
authors.initials: MJ
authors.lastname: Hung
authors.forename: Ching-Yuang
authors.initials: CY
authors.lastname: Lin
mesh_terms: D000818:Animals; D016026:Bone Marrow Transplantation; D004848:Epithelium / Q000502:physiology*; D005260:Female; D049452:Green Fluorescent Proteins; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007633:Keratins / Q000032:analysis; D051379:Mice; D010537:Peritoneum / Q000166:cytology* / Q000502:physiology; D014998:Y Chromosome
chemical_list: D049452:Green Fluorescent Proteins; D007633:Keratins