Serodiagnosis of porcine reproductive and respiratory syndrome virus infection with the use of glycoprotein 5 antigens.
Glycoprotein 5 (GP5) of porcine reproductive and respiratory syndrome virus (PRRSV) has been studied extensively as a target for vaccine development. This study evaluated the serodiagnostic application of PRRSV GP5 by enzyme-linked immunosorbent assay (ELISA). Two immunodominant peptides (VR #1 and VR #2) and two neutralizing ectodomain-containing peptides (Ecto #1 and Ecto #2), as well as recombinant GP5 (rGP5) as a control, were prepared. Serum from unvaccinated pigs was screened for the antibodies that bind to these peptide and protein antigens. The results were compared with those from a commercially available diagnostic ELISA kit (HerdChek), which uses the nucleocapsid (N) protein as an antigen. Only VR #1+#2 showed a result statistically similar to that of N protein. Ecto #1 and Ecto #2 had a lower sensitivity than VR #1+#2 and rGP5. The peptides and rGP5 showed significant associations with the N protein (P < 0.05 or 0.01), which suggests that GP5 may also be a candidate serodiagnostic antigen. Since antibodies against GP5 persist much longer than those against the N protein, GP5 itself and some of its fragments are thought to be good targets for serodiagnosis. In addition, the presence of antibodies against the PRRSV structural antigens showed significant antigen-dependent differences.
Pyo
Hyoju
H
Department of Veterinary Medicine, College of Veterinary Medicine, Chonnam National University, Gwangju 500-757, Republic of Korea.
Seo
Jayoung
J
Suh
Gukhyun
G
Kim
Kwanghyun
K
Lee
Jaeil
J
Kim
Taejung
T
D000818:Animals; D000956:Antigens, Viral / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D016056:Immunodominant Epitopes; D019318:Porcine Reproductive and Respiratory Syndrome / Q000175:diagnosis* / Q000276:immunology / Q000821:virology; D019316:Porcine respiratory and reproductive syndrome virus / Q000276:immunology / Q000502:physiology*; D012680:Sensitivity and Specificity; D012698:Serologic Tests; D013552:Swine; D014759:Viral Envelope Proteins / Q000276:immunology*
D000956:Antigens, Viral; D016056:Immunodominant Epitopes; D014759:Viral Envelope Proteins; C114789:glycoprotein 5, PRRSV