Olfactory receptor related to class A, type 2 (V1r-like Ora2) genes are conserved between distantly related rockfishes (genus Sebastes).
V1r-like Ora genes express putative chemoreceptors that may function as pheromone receptors in fishes. We used a candidate gene approach to test whether V1r-like Ora2 genes show evidence of positive selection that could suggest a role in mate recognition and the avoidance of hybridization between closely related rockfishes. We amplified a 492-bp fragment of a single V1r-like Ora2 gene from each of 5 species of rockfish. Despite separation of up to 7.8 My, the sequence of V1r-like Ora2 is highly conserved. Genetic distances are small, and all our study species shared at least one sequence with another species. Sequence comparisons suggested that, although most amino acids were subject to purifying selection, 9 amino acids showed evidence of positive selection. Because many of these amino acids were not associated with the areas of the protein suggested to be involved in ligand binding based on structural similarity to other olfactory receptors, this signal may reflect an echo of the relaxation of selection associated with the speciation events that separate these species. Strong sequence conservation suggests that this gene is of functional significance. However, because of shared alleles among species, the V1r-like Ora2 gene, in isolation, would be unlikely to differentiate species during mating season.
Johansson
Mattias L
ML
Coastal Oregon Marine Experiment Station, Hatfield Marine Science Center, Oregon State University, 2030 Southeast Marine Science Drive, Newport, OR 97365, USA. mattias.johansson@oregonstate.edu
Banks
Michael A
MA
D000595:Amino Acid Sequence; D000818:Animals; D002628:Chemoreceptor Cells; D017124:Conserved Sequence; D019143:Evolution, Molecular; D005399:Fishes / Q000145:classification* / Q000235:genetics*; D015870:Gene Expression; D008969:Molecular Sequence Data; D018034:Olfactory Receptor Neurons / Q000378:metabolism*; D010802:Phylogeny; D043563:Receptors, Pheromone / Q000235:genetics*; D020539:Sequence Analysis, Protein; D013045:Species Specificity; D019147:Vomeronasal Organ / Q000502:physiology
D043563:Receptors, Pheromone