title: Quantification of histidine, 1-methylhistidine and 3-methylhistidine in plasma and urine by capillary electrophoresis UV-detection.
abstract: We describe a new CE method with UV-detection for the quantification of histidine (His) and its methylated forms 1-methylhistidine and 3-methylhistidine, both in plasma and urine. Analytes were basically resolved using a 60 mmol/L Tris-phosphate run buffer pH 2.2 in less than 12 min. The use of a mixture of ACN/ammonia (80:20) for protein precipitation allows the quantitative recovery of all His from plasma. The optimization of the sample volume injection permits to reach an LOD of 20 nmol/L, thus improving the sensitivity of about hundred times in comparison to the previous described assays. Moreover, the opportunity to also measure creatinine in the same run makes it possible to evaluate the renal function contemporarily, thus avoiding further dosages with significant time saving. The application method has been proved by measuring His, 1-methylhistidine and 3-methylhistidine in 44 healthy subjects. In conclusion, our new method seems to be an inexpensive, fast and specific tool to assess large numbers of patients for routine analysis both in clinical and research laboratories.
authors.affiliation: Department of Biomedical Sciences and Centre of Excellence for Biotechnology Development and Biodiversity Research, University of Sassari, Sassari, Italy. angelozinellu@libero.it
authors.forename: Angelo
authors.initials: A
authors.lastname: Zinellu
authors.forename: Salvatore
authors.initials: S
authors.lastname: Sotgia
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Pisanu
authors.forename: Bastianina
authors.initials: B
authors.lastname: Scanu
authors.forename: Manuela
authors.initials: M
authors.lastname: Sanna
authors.forename: Luca
authors.initials: L
authors.lastname: Deiana
authors.forename: Ciriaco
authors.initials: C
authors.lastname: Carru
mesh_terms: D000328:Adult; D002138:Calibration; D019075:Electrophoresis, Capillary / Q000379:methods*; D005260:Female; D006639:Histidine / Q000032:analysis* / Q000097:blood / Q000652:urine; D006801:Humans; D057230:Limit of Detection; D008297:Male; D008762:Methylhistidines / Q000032:analysis* / Q000097:blood / Q000652:urine; D008875:Middle Aged; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
chemical_list: D008762:Methylhistidines; C028120:1-methylhistidine; D006639:Histidine; C028118:3-methylhistidine