Longitudinal, multimodal functional imaging of microvascular response to photothermal therapy.
Although studies have shown that photothermal therapy can coagulate selectively abnormal vasculature, the ability of this method to achieve consistent, complete removal of the vasculature is questionable. We present the use of multimodal, wide-field functional imaging to study, in greater detail, the biological response to selective laser injury. Specifically, a single-platform instrument capable of coregistered fluorescence imaging and laser speckle imaging was utilized to monitor vascular endothelial growth factor gene expression and blood flow, respectively, in a transgenic rodent model. Collectively, the longitudinal, in vivo data collected with our instrument suggest that the biological response to selective laser injury involves early-stage redistribution of blood flow, followed by increased vascular endothelial growth factor promoter activity to stimulate pro-angiogenic events.
Bui
Albert K
AK
Jefferson Medical College, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA.
Teves
Kathleen M
KM
Indrawan
Elmer
E
Jia
Wangcun
W
Choi
Bernard
B
D000818:Animals; D005786:Gene Expression Regulation / Q000528:radiation effects; D053685:Laser Therapy; D051379:Mice; D055806:Microvessels / Q000378:metabolism / Q000503:physiopathology / Q000528:radiation effects*; D057054:Molecular Imaging / Q000295:instrumentation / Q000379:methods*; D019339:Port-Wine Stain / Q000235:genetics / Q000503:physiopathology / Q000601:surgery; D013997:Time Factors; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics
D042461:Vascular Endothelial Growth Factor A