title: [Effect of vaccination against GnRH (Bopriva®) in the male pubertal calf].
abstract: The objective of this experiment was to investigate the effectiveness of a newly developed bovine anti-GnRH vaccine (Bopriva®, Pfizer Animal Health, Australia). A total of 12 peripubertal bull calves aged between 6 and 8 months were used, 2 randomly selected animals served as controls. Animals were vaccinated twice at an interval of 4 weeks with 1ml of Bopriva® (400 μg GnRH-protein-conjugate) subcutaneously in the neck and observed for a total of 36 weeks. Scrotal circumference was measured every week and blood samples were also taken weekly for the determination of testosterone and GnRH antibodies. Three months after the second injection (booster), 5 animals were slaughtered and their testes histologically examined. GnRH antibody titers rapidly began to rise after the second vaccination and reached peak values 3 weeks later. Testosterone concentrations decreased to values below 0.5 ng/ml serum 1 week after the booster and remained at this low level for at least 10 weeks. The following increase of testosterone occurred individually within 11 and 23 weeks after the booster injection. Histological examination of testes in vaccinated animals showed an incomplete spermatogenesis with impaired or no production of spermatids and a reduced diameter of seminiferous tubules. From our results we conclude that in the peripubertal bull two injections with the new bovine anti-GnRH vaccine 4 weeks apart is effective in suppressing testicular growth and testosterone secretion during at least 10 weeks after the booster injection.
authors.affiliation: Klinik für Fortpflanzungsmedizin der Universität Zürich.
authors.forename: G
authors.initials: G
authors.lastname: Theubet
authors.forename: R
authors.initials: R
authors.lastname: Thun
authors.forename: M
authors.initials: M
authors.lastname: Hilbe
authors.forename: F
authors.initials: F
authors.lastname: Janett
mesh_terms: D000818:Animals; D002417:Cattle; D007987:Gonadotropin-Releasing Hormone / Q000276:immunology*; D006801:Humans; D007117:Immunization, Secondary / Q000379:methods / Q000662:veterinary; D008297:Male; D013087:Spermatids / Q000187:drug effects; D013091:Spermatogenesis / Q000187:drug effects; D013737:Testis / Q000187:drug effects / Q000254:growth & development; D013739:Testosterone / Q000097:blood; D014611:Vaccination / Q000379:methods / Q000662:veterinary*
chemical_list: D007987:Gonadotropin-Releasing Hormone; D013739:Testosterone