title: Quantitation of sorafenib and its active metabolite sorafenib N-oxide in human plasma by liquid chromatography-tandem mass spectrometry.
abstract: A simple and rapid method with high performance liquid chromatography/tandem mass spectrometry is described for the quantitation of the kinase inhibitor sorafenib and its active metabolite sorafenib N-oxide in human plasma. A protein precipitation extraction procedure was applied to 50 μL of plasma. Chromatographic separation of the two analytes, and the internal standard [(2)H(3)(13)C]-sorafenib, was achieved on a C(18) analytical column and isocratic flow at 0.3 mL/min for 4 min. Mean within-run and between-run precision for all analytes were <6.9% and accuracy was <5.3%. Calibration curves were linear over the concentration range of 50-10,000 ng/mL for sorafenib and 10-2500 ng/mL for sorafenib N-oxide. This method allows a specific, sensitive, and reliable determination of the kinase inhibitor sorafenib and its active metabolite sorafenib N-oxide in human plasma in a single analytical run.
authors.affiliation: Department of Pharmaceutical Sciences, St. Jude Children's Research Hospital, Memphis, TN 38105, USA.
authors.forename: Lie
authors.initials: L
authors.lastname: Li
authors.forename: Ming
authors.initials: M
authors.lastname: Zhao
authors.forename: Fariba
authors.initials: F
authors.lastname: Navid
authors.forename: Keith
authors.initials: K
authors.lastname: Pratz
authors.forename: B Doug
authors.initials: BD
authors.lastname: Smith
authors.forename: Michelle A
authors.initials: MA
authors.lastname: Rudek
authors.forename: Sharyn D
authors.initials: SD
authors.lastname: Baker
mesh_terms: D001557:Benzenesulfonates / Q000097:blood* / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D009536:Niacinamide / Q000031:analogs & derivatives; D010087:Oxides / Q000097:blood* / Q000378:metabolism; D010671:Phenylurea Compounds; D011725:Pyridines / Q000097:blood* / Q000378:metabolism*; D000077157:Sorafenib; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D001557:Benzenesulfonates; D010087:Oxides; D010671:Phenylurea Compounds; D011725:Pyridines; D009536:Niacinamide; D000077157:Sorafenib