title: Validation of basophil CD164 upregulation for pollen allergy diagnosis.
abstract: The aim of the study was to evaluate the sensitivity and specificity of allergen-induced basophil CD164 upregulation in patients with seasonal allergic rhinitis caused by allergy to grass pollens. This study was performed in 24 patients with allergy to grass pollens, and in 25 healthy controls. The protocol for allergen-induced basophil CD164 upregulation consisted of whole blood samples processing and staining with anti-CCR3/anti-CD164 antibodies added to a buffer at the beginning of stimulation. We observed dose-dependent allergen-induced basophil CD164 upregulation with 100% of specificity in both used allergen concentrations (12 and 1.2 ng/ml). Higher allergen concentration resulted in 100% and lower concentration in only 70.83% sensitivity. We have observed in the patients statistically significant correlations between anti-IgE stimulation and both allergen concentrations (for 12 ng/ml, r = 0.71, p < 0.0001; and for 1.2 ng/ml, r = 0.64, p < 0.001). We conclude that assessment of allergen-induced basophil CD164 upregulation is a very useful method for in vitro determination of allergy to grass pollens. This method seems to be a very promising tool in laboratory testing of allergies to other allergens.
authors.affiliation: Department of Internal Diseases, Geriatrics and Allergology, Medical University of Wroclaw, Traugutta 57, 50-417 Wroclaw, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Wolanczyk-Medrala
authors.forename: Wojciech
authors.initials: W
authors.lastname: Barg
authors.forename: Jerzy
authors.initials: J
authors.lastname: Liebhart
authors.forename: Bernard
authors.initials: B
authors.lastname: Panaszek
authors.forename: Grazyna
authors.initials: G
authors.lastname: Nadobna
authors.forename: Marta
authors.initials: M
authors.lastname: Litwa
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Gogolewski
authors.forename: Wojciech
authors.initials: W
authors.lastname: Medrala
mesh_terms: D000328:Adult; D001491:Basophils / Q000276:immunology*; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D004306:Dose-Response Relationship, Immunologic; D051930:Endolyn / Q000097:blood; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D006109:Poaceae / Q000276:immunology*; D011058:Pollen / Q000276:immunology*; D011237:Predictive Value of Tests; D015203:Reproducibility of Results; D006255:Rhinitis, Allergic, Seasonal / Q000175:diagnosis* / Q000276:immunology; D012680:Sensitivity and Specificity; D013997:Time Factors; D015854:Up-Regulation; D055815:Young Adult
chemical_list: D015415:Biomarkers; C497898:CD164 protein, human; D051930:Endolyn