Preparation of late Drosophila embryonic fillets.
Beyond the age of ~15 h (at 25°C), Drosophila embryos become impenetrable to aqueous fixatives and staining methods as a result of cuticle deposition. This problem can be overcome using flat or fillet preparations, as described in this protocol.
Ramachandran
Preethi
P
Budnik
Vivian
V
D000818:Animals; D018929:Cell Culture Techniques; D004330:Drosophila / Q000166:cytology* / Q000196:embryology*; D006652:Histological Techniques / Q000379:methods*; D009867:Oogonia / Q000166:cytology*