title: [Infection of human papillomavirus 16/18 DNA in patients with head and neck squamous cell carcinoma and its relationship with expression of Ki-67 and P53 protein].
abstract: OBJECTIVE
  To detect the infection of human papillomavirus (HPV) 16/18 in patients with head and neck squamous cell carcinoma and explore the relationship between HPV infection and expressions of Ki-67 and P53 proteins in tumor tissue.
  
  
  METHODS
  The level of HPV 16/18 DNA was measured by real time polymerase chain reaction, and Ki-67 and P53 proteins were measured by immunohistochemistry in tissues from head and neck squamous cell carcinoma.
  
  
  RESULTS
  HPV 16/18 DNA was detected in 62.8% of our patients. In each cancer tissue sample, Ki-67 protein was expressed between 2% to 70%. P53 protein was expressed in 46.15% of our patients. No significant relation was found between HPV 16/18 DNA level and sex, smoking, drinking, and tumor clinical stages. However, level of HPV 16/18 DNA was found to have positive relation with tumor pathological grades and negative relation with P53 protein expression. No relation with Ki-67 protein expression was found.
  
  
  CONCLUSIONS
  Head and neck squamous cell carcinoma may be initiated by HPV 16/18 infection and the mechanism in carcinogenesis involves abnormal expression in P53 protein.
authors.affiliation: Department of Medical Oncology, PUMC Hospital, CAMS and PUMC, Beijing 100730, China.
authors.forename: Jie-li
authors.initials: JL
authors.lastname: Zhang
authors.forename: Zhao
authors.initials: Z
authors.lastname: Sun
authors.forename: Zhen
authors.initials: Z
authors.lastname: Huo
authors.forename: Yu-feng
authors.initials: YF
authors.lastname: Luo
authors.forename: Shui-qing
authors.initials: SQ
authors.lastname: Ma
authors.forename: De-tian
authors.initials: DT
authors.lastname: Wang
authors.forename: Jin-ling
authors.initials: JL
authors.lastname: Cao
authors.forename: Ti
authors.initials: T
authors.lastname: Yang
authors.forename: Quan-cai
authors.initials: QC
authors.lastname: Cui
authors.forename: Chun-mei
authors.initials: CM
authors.lastname: Bai
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000821:virology*; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D052162:Human papillomavirus 16 / Q000302:isolation & purification*; D052161:Human papillomavirus 18 / Q000302:isolation & purification*; D006801:Humans; D019394:Ki-67 Antigen / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*; D002583:Uterine Cervical Neoplasms / Q000378:metabolism / Q000821:virology*
chemical_list: D004279:DNA, Viral; D019394:Ki-67 Antigen; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53