title: Full-field time-resolved fluorescence tomography of small animals.
abstract: In this experimental investigation, we explore the feasibility of using wide-field illumination for time-resolved fluorescence molecular tomography. The performance of wide-field patterns with a time-resolved imaging platform is investigated in vitro and in a small animal model. A Monte Carlo-based forward model is employed to reconstruct fluorescence yield based on time-gated datasets. An improvement in resolution and quantification when using the time-gate data type compared to the commonly used cw data type is demonstrated in vitro. Furthermore, the feasibility of wide-field strategies for fluorescence preclinical applications is established by an accurate localization of a fluorescent inclusion implanted in the chest cavity of a murine model.
authors.affiliation: Department of Biomedical Engineering, Rensselaer Polytechnic Institute, Troy, New York 12180, USA.
authors.forename: Vivek
authors.initials: V
authors.lastname: Venugopal
authors.forename: Jin
authors.initials: J
authors.lastname: Chen
authors.forename: Frederic
authors.initials: F
authors.lastname: Lesage
authors.forename: Xavier
authors.initials: X
authors.lastname: Intes
mesh_terms: D000818:Animals; D005240:Feasibility Studies; D021621:Imaging, Three-Dimensional; D051379:Mice; D009010:Monte Carlo Method; D019047:Phantoms, Imaging; D013050:Spectrometry, Fluorescence / Q000379:methods*; D014054:Tomography / Q000379:methods*