Synthesis, characterization and cytotoxicity studies of chitosan-coated tea polyphenols nanoparticles.
Chitosan nanoparticles (CS-NPs) were prepared by ionic gelation method using carboxymethyl chitosan and chitosan hydrochloride as carriers of tea polyphenols. The characteristics of chitosan-coated tea polyphenols nanoparticles (CS-TP NPs) were determined by using transmission electron microscopy (TEM) and FT-IR spectroscopy. It was found that the synthesized CS-TP NPs were non-spherical in shape with an average size of 407±50nm. Meanwhile, the drug content and encapsulation rate of the nanoparticles was 8-16% and 44-83%, respectively. These CS-TP NPs also demonstrated sustained release of tea polyphenols in PBS. The antitumor of CS-TP NPs towards HepG2 cancer cells was investigated. The result showed that CS-TP NPs retained significant antitumor activities.
Liang
Jin
J
College of Food Science and Technology, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China.
Li
Feng
F
Fang
Yong
Y
Yang
Wenjian
W
An
Xinxin
X
Zhao
Liyan
L
Xin
Zhihong
Z
Cao
Lin
L
Hu
Qiuhui
Q
D048271:Chitosan / Q000031:analogs & derivatives / Q000737:chemistry*; D002712:Chlorides / Q000737:chemistry; D005419:Flavonoids / Q000737:chemistry*; D056945:Hep G2 Cells; D006801:Humans; D007477:Ions; D008027:Light; D046529:Microscopy, Electron, Transmission / Q000379:methods; D053758:Nanoparticles / Q000737:chemistry; D036103:Nanotechnology / Q000379:methods; D010636:Phenols / Q000737:chemistry*; D059808:Polyphenols; D012542:Scattering, Radiation; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D013662:Tea; D013778:Tetrazolium Salts / Q000494:pharmacology; D013844:Thiazoles / Q000494:pharmacology
D002712:Chlorides; D005419:Flavonoids; D007477:Ions; D010636:Phenols; D059808:Polyphenols; D013662:Tea; D013778:Tetrazolium Salts; D013844:Thiazoles; C514968:carboxymethyl-chitosan; D048271:Chitosan; C022616:thiazolyl blue