title: The paradox of multiplex DNA melting on a surface.
abstract: Under equilibrium conditions, there are two regimes of target capture on a surface--target limited and probe limited. In the probe limited regime, the melting curve from multiplex target dissociation from the surface exhibits a single transition due to a reverse displacement mechanism of the low affinity species. The melting curve cannot be used in analytical methods to resolve heteroduplexes; only with the simplex system can proper thermodynamics be obtained.
authors.affiliation: Department of Electrical and Computer Engineering, University of Utah, USA.
authors.forename: Layne
authors.initials: L
authors.lastname: Williams
authors.forename: Steve
authors.initials: S
authors.lastname: Blair
authors.forename: Alexander
authors.initials: A
authors.lastname: Chagovetz
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Fish
authors.forename: Albert S
authors.initials: AS
authors.lastname: Benight
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D009691:Nucleic Acid Denaturation; D013816:Thermodynamics; D044366:Transition Temperature
chemical_list: D004247:DNA