Total chemical synthesis and X-ray structure of kaliotoxin by racemic protein crystallography.
Here we report the total synthesis of kaliotoxin by 'one pot' native chemical ligation of three synthetic peptides. A racemic mixture of D- and L-kaliotoxin synthetic protein molecules gave crystals in the centrosymmetric space group P1 that diffracted to atomic-resolution (0.95 Å), enabling the X-ray structure of kaliotoxin to be determined by direct methods.
Pentelute
Brad L
BL
Department of Chemistry, Institute for Biophysical Dynamics, The University of Chicago, Chicago, Illinois 60637, USA.
Mandal
Kalyaneswar
K
Gates
Zachary P
ZP
Sawaya
Michael R
MR
Yeates
Todd O
TO
Kent
Stephen B H
SB
D000595:Amino Acid Sequence; D018360:Crystallography, X-Ray; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry; D017434:Protein Structure, Tertiary; D012604:Scorpion Venoms / Q000138:chemical synthesis* / Q000737:chemistry; D013237:Stereoisomerism
D010455:Peptides; D012604:Scorpion Venoms; C072276:kaliotoxin