title: Interactive proteomics research technologies: recent applications and advances.
abstract: Proteins rarely exert their function alone. They normally function in multiprotein complexes that play central roles in all biological functions. Thus, it is not surprising that the investigation of protein-protein interactions on a global scale, of so-called interactomes, has become crucial to modern molecular biology. Dissecting partners in protein complexes gives insight into their molecular function and can help in understanding disease-related mechanisms, ultimately resulting in better drug target definition. A variety of methods exist to unravel protein interaction circuitries and recently, significant progress has been made in adapting these tools for the generation of large-scale interaction datasets. Here, we present an overview of the latest advances and applications of interactive proteomics research technologies.
authors.affiliation: Department of Biochemistry & Department of Molecular Genetics, Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 160 College Street, Toronto, Ontario, Canada.
authors.forename: Julia
authors.initials: J
authors.lastname: Petschnigg
authors.forename: Jamie
authors.initials: J
authors.lastname: Snider
authors.forename: Igor
authors.initials: I
authors.lastname: Stagljar
mesh_terms: D000818:Animals; D015870:Gene Expression; D057166:High-Throughput Screening Assays; D006801:Humans; D058990:Molecular Targeted Therapy; D046912:Multiprotein Complexes / Q000378:metabolism*; D010446:Peptide Fragments / Q000378:metabolism; D019151:Peptide Library; D040081:Protein Array Analysis; D011485:Protein Binding; D025941:Protein Interaction Mapping; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D020543:Proteome / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D020798:Two-Hybrid System Techniques
chemical_list: D046912:Multiprotein Complexes; D010446:Peptide Fragments; D019151:Peptide Library; D011506:Proteins; D020543:Proteome