title: The effects of T4 and A3/R phage preparations on whole-blood monocyte and neutrophil respiratory burst.
abstract: Bacteriophages (viruses of bacteria) are currently considered a promising means of treating antibiotic-resistant infections. The main objective of this study was to evaluate the intensity of the whole-blood monocyte and neutrophil respiratory burst induced by purified preparations and lysates of the bacteriophages T4 and A3/R. While A3/R phage preparations did not induce a significant respiratory burst, T4 phage preparations increased the production of reactive oxygen species in a dose-dependent manner. However, the intensity of the phage-induced respiratory burst was much lower than that triggered by heat-inactivated Staphylococcus aureus cells. These results suggest that phage preparations are not likely to induce oxidative stress following their administration to patients.
authors.affiliation: Department of Clinical Immunology, Transplantation Institute, Warsaw Medical University, Warsaw, Poland. jborysowski@interia.pl
authors.forename: Jan
authors.initials: J
authors.lastname: Borysowski
authors.forename: Piotr
authors.initials: P
authors.lastname: Wierzbicki
authors.forename: Danuta
authors.initials: D
authors.lastname: Kłosowska
authors.forename: Grazyna
authors.initials: G
authors.lastname: Korczak-Kowalska
authors.forename: Beata
authors.initials: B
authors.lastname: Weber-Dabrowska
authors.forename: Andrzej
authors.initials: A
authors.lastname: Górski
mesh_terms: D001435:Bacteriophages / Q000276:immunology*; D006801:Humans; D009000:Monocytes / Q000276:immunology*; D009504:Neutrophils / Q000276:immunology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D016897:Respiratory Burst; D013211:Staphylococcus aureus / Q000276:immunology
chemical_list: D017382:Reactive Oxygen Species