Optical genetic mapping defines regions of chromosomal variation in serovars of S. enterica subsp. enterica of concern for human and animal health.
Infections involving Salmonella enterica subsp. enterica serovars have serious animal and human health implications; causing gastroenteritis in humans and clinical symptoms, such as diarrhoea and abortion, in livestock. In this study an optical genetic mapping technique was used to screen 20 field isolate strains from four serovars implicated in disease outbreaks. The technique was able to distinguish between the serovars and the available sequenced strains and group them in agreement with similar data from microarrays and PFGE. The optical maps revealed variation in genome maps associated with antimicrobial resistance and prophage content in S. Typhimurium, and separated the S. Newport strains into two clear geographical lineages defined by the presence of prophage sequences. The technique was also able to detect novel insertions that may have had effects on the central metabolism of some strains. Overall optical mapping allowed a greater level of differentiation of genomic content and spatial information than more traditional typing methods.
Saunders
M P
MP
Department of Food and Environmental Safety (FES), Veterinary Laboratories Agency (Weybridge), Addlestone, Surrey, UK.
Wu
G
G
Department of Food and Environmental Safety (FES), Veterinary Laboratories Agency (Weybridge), Addlestone, Surrey, UK.
Abuoun
M
M
Department of Food and Environmental Safety (FES), Veterinary Laboratories Agency (Weybridge), Addlestone, Surrey, UK.
Pan
Z
Z
Department of Food and Environmental Safety (FES), Veterinary Laboratories Agency (Weybridge), Addlestone, Surrey, UK.
Anjum
M
M
Department of Food and Environmental Safety (FES), Veterinary Laboratories Agency (Weybridge), Addlestone, Surrey, UK.
Woodward
M J
MJ
Department of Food and Environmental Safety (FES), Veterinary Laboratories Agency (Weybridge), Addlestone, Surrey, UK.
D000818:Animals; D002874:Chromosome Mapping / Q000379:methods; D002876:Chromosomes, Bacterial / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field; D014644:Genetic Variation / Q000235:genetics; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D012480:Salmonella Infections / Q000382:microbiology; D012481:Salmonella Infections, Animal / Q000382:microbiology; D019779:Salmonella enterica / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity; D012703:Serotyping / Q000379:methods