title: Site-specific biomolecule labeling with gold clusters.
abstract: Site-specific labeling of biomolecules in vitro with gold clusters can enhance the information content of electron cryomicroscopy experiments. This chapter provides a practical overview of well-established techniques for forming biomolecule/gold cluster conjugates. Three bioconjugation chemistries are covered: linker-mediated bioconjugation, direct gold-biomolecule bonding, and coordination-mediated bonding of nickel(II) nitrilotriacetic acid (NTA)-derivatized gold clusters to polyhistidine (His)-tagged proteins.
authors.affiliation: Colorado State University, Department of Chemistry, Fort Collins, Colorado, USA.
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Ackerson
authors.forename: Richard D
authors.initials: RD
authors.lastname: Powell
authors.forename: James F
authors.initials: JF
authors.lastname: Hainfeld
mesh_terms: D020285:Cryoelectron Microscopy / Q000379:methods*; D006046:Gold / Q000737:chemistry*; D006639:Histidine / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry*; D009571:Nitrilotriacetic Acid / Q000737:chemistry; D011506:Proteins / Q000737:chemistry / Q000648:ultrastructure
chemical_list: D011506:Proteins; C033223:polyhistidine; D006639:Histidine; D006046:Gold; D009571:Nitrilotriacetic Acid