Elaboration of spin-coated cellulose-xyloglucan multilayered thin films.
In the context of developing a biomimetic model of the primary cell wall, our aim was to produce multilayered thin films composed of cellulose nanocrystals (CN) and xyloglucan (XG). We investigated the effect of XG concentrations ranging from 0.5 g/L to 10 g/L. The choice of concentration was based on rheological investigation of the XG solutions which indicated that the two lower concentrations (0.5 and 1 g/L) correspond to a semidilute regime where the polymer chains are not entangled, whereas they are entangled at the highest concentrations (5 and 10 g/L). Several processes of film preparation were tested (dipping or spin-coating, with or without a rinsing step). The film growth profiles obtained for different XG concentrations by mechanical profilometry showed that spin-coating without rinsing was the most efficient process. Results showed that at high XG concentrations (XG = 5 g/L and XG = 10 g/L) plateau values were reached after the formation of 3 or 4 bilayers, whereas growth of the multilayer structure was linear at the lower XG concentrations (XG = 0.5 g/L and XG = 1 g/L). The thickness of one CN/XG bilayer corresponded to a single layer of CN covered by a thin XG layer, despite the absence of a rinsing step between successive coatings. The importance of the XG concentration was confirmed by determining by neutron reflectivity the film architecture obtained from four XG solutions after eight successive paired coatings. The results are discussed in relation to the role of XG in the plant cell wall.
Cerclier
Carole
C
UR1268 Biopolymères Interactions Assemblages, INRA, F-44316 Nantes, France.
Cousin
Fabrice
F
Bizot
Hervé
H
Moreau
Céline
C
Cathala
Bernard
B
D000327:Adsorption; D040761:Biomimetic Materials / Q000737:chemistry*; D002482:Cellulose / Q000737:chemistry*; D005936:Glucans / Q000737:chemistry*; D007700:Kinetics; D033363:Neutron Diffraction; D029861:Tamarindus / Q000737:chemistry; D014783:Viscosity; D014990:Xylans / Q000737:chemistry*
D005936:Glucans; D014990:Xylans; C029353:xyloglucan; D002482:Cellulose