title: [Aminoglycoside resistance in Enterobacteriaceae originated in a gynecology-obstetrical hospital: a survey for 16s rRNA methylases: ArmA, RmtB and RmtC].
abstract: We examined the resistance of 2359 clinical isolates of Enterobacteriaceae to gentamicin, amikacin, netilmicin and neomycin by the disc-diffusion assay. The isolates originated from female-patients and newborn infants in a gynecology-obstetrical hospital in Warsaw, Poland. Isolates from adults predominated. Most of the isolated bacteria were considered non-pathogenic, colonization flora. The majority (above 95%) of the isolates were sensitive to all of the tested aminoglycosides. Forty five (1,90%) isolates were resistant to one or more agents. In this group, E. coli was prevalent (73,33%). As little as 14 isolates had no growth inhibition around the gentamicin disc (10 mcg) (contact-growth). MICs of seven isolates ranged from 256 to 1024 (mcg/ml) of the tested agents. One isolate had MIC 1024 for amikacin and kanamycin. All the contact-growth isolates were examined for genes encoding for TEM, SHV and CTX-M beta-lactamases, and genes armA, rmtB and rmtC for 16S rRNA methylases reported in Europe. All of them produced TEM enzyme while SHV and CTX-M was expressed by one and two isolates respectively. None of the tested 16S rRNA methylases was detected. Our results show the low carriage of the aminoglycoside-resistant Enterobacteriaceae in healthy pregnant-women and most probably their sexual partners. Our findings may argue that production of the 16S rRNA methylases is still limited to patients with a long-term antibiotic-therapy in regular hospitals rather than to non-hospitalized population in Poland.
authors.affiliation: Zakład Bakteriologii Narodowego Instytutu Zdrowia Publicznego--Państwowego Zakładu Higieny.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Zacharczuk
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Piekarska
authors.forename: Jolanta
authors.initials: J
authors.lastname: Szych
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Sulik
authors.forename: Ewa
authors.initials: E
authors.lastname: Roszko
authors.forename: Joanna
authors.initials: J
authors.lastname: Łata
authors.forename: Marek
authors.initials: M
authors.lastname: Jagielski
authors.forename: Rafal
authors.initials: R
authors.lastname: Gierczyński
mesh_terms: D000328:Adult; D000617:Aminoglycosides / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial; D004755:Enterobacteriaceae / Q000145:classification / Q000187:drug effects* / Q000201:enzymology* / Q000302:isolation & purification; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D008780:Methyltransferases / Q000032:analysis* / Q000235:genetics; D009775:Obstetrics and Gynecology Department, Hospital; D011044:Poland; D011247:Pregnancy; D013045:Species Specificity
chemical_list: D000617:Aminoglycosides; D008780:Methyltransferases; C047346:16S rRNA (adenine(1518)-N(6)-adenine(1519)-N(6))-dimethyltransferase