Overcoming glucose repression in mixed sugar fermentation by co-expressing a cellobiose transporter and a β-glucosidase in Saccharomyces cerevisiae.
Glucose repression is one of the main limitations in mixed lignocellulosic sugar fermentation for cost-effective production of fuels and chemicals. Here we report a novel strategy to overcome glucose repression by co-expressing a cellobiose transporter and a β-glucosidase in an engineered d-xylose-utilizing Saccharomyces cerevisiae strain. The resulting strain can simultaneously utilize cellobiose and d-xylose for ethanol production.
Li
Sijin
S
Energy Biosciences Institute, Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, 600 S. Mathews Ave., Urbana IL 61801, USA.
Du
Jing
J
Sun
Jie
J
Galazka
Jonathan M
JM
Glass
N Louise
NL
Cate
Jamie H D
JH
Yang
Xiaomin
X
Zhao
Huimin
H
D018533:Biomass; D050260:Carbohydrate Metabolism; D002475:Cellobiose / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism; D005285:Fermentation; D005947:Glucose / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000378:metabolism*; D008954:Models, Biological; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000378:metabolism; D014994:Xylose / Q000378:metabolism; D001617:beta-Glucosidase / Q000378:metabolism*
D026901:Membrane Transport Proteins; D002475:Cellobiose; D000431:Ethanol; D014994:Xylose; D001617:beta-Glucosidase; D005947:Glucose