Enzymatic synthesis of L-tryptophan from hair acid hydrolysis industries wastewater with tryptophan synthase.
An effective method for production of L-tryptophan from hair acid hydrolysis wastewater (HHW) containing L-serine was developed by recombinant tryptophan synthase. This study provides us with an alternative HHW utilization strategy. Tryptophan synthase could efficiently convert L-serine contained in HHW to L-tryptophan at pH 8.0, 40°C and Tween-80 of 0.04%. The enzyme also showed high tolerance to ammonium chloride, a component in HHW. In a scale up study, L-serine conversion rate reach 95.1% with a final L-tryptophan concentration of 33.2 g l(-1).
Zhao
Genhai
G
State Key Laboratory of Pharmaceutical Biotechnology, School of Life Science, Nanjing University, Nanjing, People's Republic of China.
Liu
Junzhong
J
Dong
Kai
K
Zhang
Fei
F
Zhang
Hongjuan
H
Liu
Qian
Q
Jiao
Qingcai
Q
D000143:Acids / Q000737:chemistry*; D006197:Hair / Q000737:chemistry*; D006868:Hydrolysis; D007220:Industrial Waste / Q000517:prevention & control*; D012694:Serine / Q000737:chemistry*; D014364:Tryptophan / Q000138:chemical synthesis*; D014367:Tryptophan Synthase / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000737:chemistry*
D000143:Acids; D007220:Industrial Waste; D014874:Water Pollutants, Chemical; D012694:Serine; D014364:Tryptophan; D014367:Tryptophan Synthase