title: Investigation of MADS domain transcription factor dynamics in the floral meristem.
abstract: To study the importance of intercellular transport for MADS domain transcription factor functioning during floral development, we analyzed the dynamic behavior of fluorescently-tagged MADS domain proteins in transgenic plants by Confocal Laser Scanning Microscopy. These analyses, described in a recent paper in The Plant Journal, provided proof for previous suggestions that the Arabidopsis thaliana C-type protein AGAMOUS has a non-cell-autonomous role in floral meristem integrity. Furthermore, it indicated a possible non-cell-autonomous role for the B-type proteins APETALA3 and PISTILLATA, and the E-type protein SEPALLATA3, through lateral intercellular movement in the floral meristem. In this addendum we compare some of the available fluorescent protein-based technologies for the investigation of transcription factor movements and dynamics.
authors.affiliation: Plant Research International, Droevendaalsesteeg, The Netherlands.
authors.forename: Susan L
authors.initials: SL
authors.lastname: Urbanus
authors.forename: Q D Peter
authors.initials: QD
authors.lastname: Dinh
authors.forename: Gerco C
authors.initials: GC
authors.lastname: Angenent
authors.forename: Richard G H
authors.initials: RG
authors.lastname: Immink
mesh_terms: D017360:Arabidopsis / Q000378:metabolism* / Q000528:radiation effects; D029681:Arabidopsis Proteins / Q000378:metabolism*; D035264:Flowers / Q000378:metabolism* / Q000528:radiation effects; D036681:Fluorescence Recovery After Photobleaching; D005456:Fluorescent Dyes / Q000378:metabolism; D008027:Light; D026161:MADS Domain Proteins / Q000378:metabolism*; D018519:Meristem / Q000378:metabolism* / Q000528:radiation effects
chemical_list: C091535:AP1 protein, Arabidopsis; D029681:Arabidopsis Proteins; D005456:Fluorescent Dyes; D026161:MADS Domain Proteins