title: Cutting edge: CD49d+ neutrophils induce FcepsilonRI expression on lung dendritic cells in a mouse model of postviral asthma.
abstract: The increasing prevalence of atopy and asthma remains unexplained but may be due to infection with respiratory viruses. In support of this hypothesis, we showed that experimental asthma after viral infection in mice depended on type I IFN-driven upregulation of FcεRI on conventional dendritic cells (cDCs) in the lung. In this article, we demonstrate that FcεRI expression on lung cDCs depends on an unexpected activity of a CD49d(+) subset of polymorphonuclear neutrophils (PMNs) that are found in the lungs of wild-type C57BL6 but not mice deficient in type I IFNR. Expression of FcεRI depends in part on a CD11b-dependent interaction between PMNs and cDCs. This study demonstrates a PMN-cDC interaction in the lung that is necessary for the ability of viral infection to induce atopic disease.
authors.affiliation: Division of Allergy and Clinical Immunology, Department of Pediatrics, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
authors.forename: Dorothy S
authors.initials: DS
authors.lastname: Cheung
authors.forename: Sarah J
authors.initials: SJ
authors.lastname: Ehlenbach
authors.forename: Robert T
authors.initials: RT
authors.lastname: Kitchens
authors.forename: Desiré A
authors.initials: DA
authors.lastname: Riley
authors.forename: Larry L
authors.initials: LL
authors.lastname: Thomas
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Holtzman
authors.forename: Mitchell H
authors.initials: MH
authors.lastname: Grayson
mesh_terms: D000818:Animals; D001249:Asthma / Q000276:immunology* / Q000821:virology; D002469:Cell Separation; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism; D004195:Disease Models, Animal; D005434:Flow Cytometry; D007150:Immunohistochemistry; D039441:Integrin alpha4 / Q000096:biosynthesis / Q000276:immunology*; D008168:Lung / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009504:Neutrophils / Q000276:immunology* / Q000378:metabolism; D053633:Receptor, Interferon alpha-beta / Q000172:deficiency / Q000276:immunology; D017455:Receptors, IgE / Q000096:biosynthesis / Q000276:immunology*; D012141:Respiratory Tract Infections / Q000150:complications / Q000276:immunology; D010253:Respirovirus Infections / Q000150:complications / Q000276:immunology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D029082:Sendai virus
chemical_list: C507964:IFNAR1 protein, human; D017455:Receptors, IgE; D039441:Integrin alpha4; D053633:Receptor, Interferon alpha-beta