Activation of PKC-alpha is required for migration of C6 glioma cells.
The PKC signaling pathway has been implicated in diverse cellular functions. Here, we sought to investigate the role of PKC-alpha÷beta in C6 glioma cell migration. We found that both PKC-alpha and PKC-beta were expressed by C6 glioma cells, but only PKC-alpha was markedly activated in serum-treated C6 cells. Go6976, a PKC-alpha÷beta specific inhibitor, was found to cause a dose-dependent reduction of PKC-alpha activation and cell migration induced by serum in C6 cells. These results collectively indicated that the PKC-alpha signaling pathway is necessary for glioma cell migration. Our findings may provide an insight into a better understanding to the malignant progression of gliomas.
Hu
Jian-Guo
JG
Anhui Key Laboratory of Tissue Transplantation, Bengbu Medical College, Bengbu, China. jghu.hzlv@yahoo.com.
Wang
Xiao-Fei
XF
Zhou
Jian-Sheng
JS
Wang
Feng-Chao
FC
Li
Xing-Wu
XW
Lü
He-Zuo
HZ
D000818:Animals; D000963:Antimetabolites; D015153:Blotting, Western; D001932:Brain Neoplasms / Q000378:metabolism / Q000473:pathology; D001973:Bromodeoxyuridine; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology*; D004789:Enzyme Activation; D005910:Glioma / Q000378:metabolism / Q000473:pathology; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D051571:Protein Kinase C-alpha / Q000037:antagonists & inhibitors / Q000378:metabolism*; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D051381:Rats
D000963:Antimetabolites; D047428:Protein Kinase Inhibitors; D051571:Protein Kinase C-alpha; D001973:Bromodeoxyuridine