title: Simultaneous detection of attomolar pathogen DNAs by Bio-MassCode mass spectrometry.
abstract: A general method is described to analyze pathogen DNAs in homogenous solution by a novel Bio-MassCode probe, using a small organic (namely disulfide) "reporter mass" that self-assembled in oligonucleotide-modified gold nanoparticles. With conventional MALDI TOF MS, the assay simultaneously detected DNAs for HIV, HBV, HCV, and TP with extraordinarily high sensitivity in the low attomolar (10(-18) M) range without any other amplification.
authors.affiliation: Institute of Molecular Medicine and School of Medicine, Nanjing University, Nanjing 210093, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Yang
authors.forename: Kai
authors.initials: K
authors.lastname: Gu
authors.forename: Xiaoru
authors.initials: X
authors.lastname: Sun
authors.forename: Huan
authors.initials: H
authors.lastname: Huang
authors.forename: Yibing
authors.initials: Y
authors.lastname: Ding
authors.forename: Fuqiang
authors.initials: F
authors.lastname: Wang
authors.forename: Guohua
authors.initials: G
authors.lastname: Zhou
authors.forename: Lequn Lee
authors.initials: LL
authors.lastname: Huang
mesh_terms: D004279:DNA, Viral / Q000032:analysis*; D006046:Gold / Q000737:chemistry; D006678:HIV / Q000235:genetics; D016174:Hepacivirus / Q000235:genetics; D006515:Hepatitis B virus / Q000235:genetics; D053768:Metal Nanoparticles / Q000737:chemistry; D009841:Oligonucleotides / Q000737:chemistry; D012367:RNA, Viral / Q000032:analysis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D004279:DNA, Viral; D009841:Oligonucleotides; D012367:RNA, Viral; D006046:Gold