The effects of T4 and A3/R phage preparations on whole-blood monocyte and neutrophil respiratory burst.
Bacteriophages (viruses of bacteria) are currently considered a promising means of treating antibiotic-resistant infections. The main objective of this study was to evaluate the intensity of the whole-blood monocyte and neutrophil respiratory burst induced by purified preparations and lysates of the bacteriophages T4 and A3/R. While A3/R phage preparations did not induce a significant respiratory burst, T4 phage preparations increased the production of reactive oxygen species in a dose-dependent manner. However, the intensity of the phage-induced respiratory burst was much lower than that triggered by heat-inactivated Staphylococcus aureus cells. These results suggest that phage preparations are not likely to induce oxidative stress following their administration to patients.
Borysowski
Jan
J
Department of Clinical Immunology, Transplantation Institute, Warsaw Medical University, Warsaw, Poland. jborysowski@interia.pl
Wierzbicki
Piotr
P
Kłosowska
Danuta
D
Korczak-Kowalska
Grazyna
G
Weber-Dabrowska
Beata
B
Górski
Andrzej
A
D001435:Bacteriophages / Q000276:immunology*; D006801:Humans; D009000:Monocytes / Q000276:immunology*; D009504:Neutrophils / Q000276:immunology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D016897:Respiratory Burst; D013211:Staphylococcus aureus / Q000276:immunology
D017382:Reactive Oxygen Species