title: [Study on the deletion and mutation of p16 gene in human non-small cell lung cancer.].
abstract: BACKGROUND
  To study the relationship between the deletion and mutation of p16 gene and clinicopathologic manifestations of human non2small cell lung cancer (NSCLC) .
  
  
  METHODS
  Silver staining PCR-SSCP method of control amount of template DNA was used to detect exon 2 of p16 gene in human NSCLC tissue specimens.
  
  
  RESULTS
  The deletion of p16 gene was identified in 13 out of 40 specimens. Of the 40 specimens , 3 showed a variant band indicative of the mutation. There was significant correlation between the frequency of the deletion and mutation (40 %) and clinicopathologic stage ( P < 0. 05) .
  
  
  CONCLUSIONS
  The deletion and mutation of p16 gene may play an important role in carcinogenesis ,development and metastasis of human NSCLC.
authors.affiliation: Department of Thoracic Surgery , The First Affiliated Hospital , Chongqing Medical University , Chongqing 400016 , P. R. China.
authors.forename: M
authors.initials: M
authors.lastname: Du
authors.forename: L
authors.initials: L
authors.lastname: Li
authors.forename: Z
authors.initials: Z
authors.lastname: Zeng
authors.forename: X
authors.initials: X
authors.lastname: Zhang