title: Stringent programming of DNA methylation in humans.
abstract: We describe a PCR-based method called Amplified Methylation Polymorphism (AMP) for scanning genomes for DNA methylation changes. AMP detects tissue-specific DNA methylation signatures often representing junctions between methylated and unmethylated DNA close to intronexon junctions and/or associated with CpG islands. Identical AMP profiles are detected for healthy, young, monozygotic twins.
authors.affiliation: School of Chemistry and Molecular Biosciences, The University of Queensland, Australia.
authors.forename: Hnin T
authors.initials: HT
authors.lastname: Aung
authors.forename: Dion K
authors.initials: DK
authors.lastname: Harrison
authors.forename: Ian
authors.initials: I
authors.lastname: Findlay
authors.forename: John S
authors.initials: JS
authors.lastname: Mattick
authors.forename: Nicholas G
authors.initials: NG
authors.lastname: Martin
authors.forename: Bernard J
authors.initials: BJ
authors.lastname: Carroll
mesh_terms: D000293:Adolescent; D000818:Animals; D001483:Base Sequence; D018899:CpG Islands; D019175:DNA Methylation / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D005091:Exons; D005819:Genetic Markers; D006801:Humans; D007438:Introns; D051379:Mice; D021141:Nucleic Acid Amplification Techniques / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D014430:Twins, Monozygotic / Q000235:genetics*
chemical_list: D017931:DNA Primers; D005819:Genetic Markers