title: The potential of synthetic thiourea compound to reduce the cytotoxic and genotoxic effects of paraquat in Hordeum vulgare and cultured human lymphocytes.
abstract: This work evaluated the ability of one synthetic compound 1-(4-fluorophenylthiocarbamoyl)-4-methyl-piperazine (FTMP), thiourea derivative to reduce cytotoxic and genotoxic effects of free radical inducer paraquat (PQ) in two different test systems Hordeum vulgare and human lymphocytes in vitro. The mitotic index was used as a marker for cytotoxicity. To indicate genotoxicity, chromosome aberrations test and micronucleus induction test were used. FTMP manifested a weak genotoxic effect in both test systems. Clear evidence was obtained that conditioning treatment with FTMP (10(-6) , 5 × 10(-6) , and 10(-5) mol/l) could decrease chromosome aberrations and micronuclei induced by PQ in both test systems. "Aberration hot spots" in heterochromatin containing segments were reduced. The present data show that the thiourea synthetic compound FTMP provides genome protection against the harmful action of oxidative stress inductor PQ. Human lymphocytes were found to be more sensitive to the cytotoxic and clastogenic effects of FTMP conditioning treatment than Hordeum vulgare. Revealing the protective action of newly synthesized compounds could contribute to the improvement of our present knowledge of the mechanisms of mutagenesis and antimutagenesis.
authors.affiliation: Department of Bioindication and Risk Assessment, Central Laboratory of General Ecology, Bulgarian Academy of Sciences, 2 Gagarin Street, Sofia 1113, Bulgaria. spetkova2002@yahoo.co.uk
authors.forename: Svetla
authors.initials: S
authors.lastname: Gateva
authors.forename: Gabriele
authors.initials: G
authors.lastname: Jovtchev
authors.forename: Mila
authors.initials: M
authors.lastname: Stergios
authors.forename: Petranka
authors.initials: P
authors.lastname: Yonova
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000494:pharmacology*; D004249:DNA Damage / Q000187:drug effects*; D005260:Female; D001467:Hordeum / Q000166:cytology / Q000187:drug effects* / Q000235:genetics; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D015162:Micronucleus Tests; D008875:Middle Aged; D009153:Mutagens / Q000633:toxicity; D010269:Paraquat / Q000633:toxicity*; D010879:Piperazines / Q000494:pharmacology*; D013890:Thiourea / Q000031:analogs & derivatives* / Q000494:pharmacology*
chemical_list: C574243:1-(4-fluorophenylthiocarbamoyl)-4-methylpiperazine; D000975:Antioxidants; D009153:Mutagens; D010879:Piperazines; D013890:Thiourea; D010269:Paraquat