title: Modern determination of complement activation.
abstract: Modern, that is, accurate determination of complement activation in vivo requires the simultaneous determination of both naive and activated complement proteins. This is best achieved by the use of native-restricted and neoepitope-specific monoclonal antibodies in sensitive enzyme-linked immunosorbent assays. Several confounding factors also have to be taken into account to allow a correct interpretation of the results.
authors.affiliation: Abteilung Zelluläre und Molekulare Immunologie, Zentrum für Hygiene und Humangenetik, Humboldtallee 34, Göttingen, Germany. mopperm@gwdg.de
authors.forename: Martin
authors.initials: M
authors.lastname: Oppermann
authors.forename: Reinhard
authors.initials: R
authors.lastname: Würzner
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D003167:Complement Activation / Q000276:immunology*; D015941:Complement Hemolytic Activity Assay / Q000379:methods; D003171:Complement Pathway, Classical / Q000276:immunology*; D003165:Complement System Proteins / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D011485:Protein Binding; D011951:Receptors, Complement / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D011951:Receptors, Complement; D003165:Complement System Proteins