title: Action of propolis and medications against Escherichia coli and endotoxin in root canals.
abstract: This study evaluated the action of propolis and intracanal medications against Escherichia coli and endotoxin. Forty-eight dental roots were contaminated with E. coli. The root canals were instrumented with propolis and divided into groups according to the type of intracanal medication: Ca(OH)(2), polymyxin B, or Ca(OH)(2) + 2% chlorhexidine gel. In the control group, saline solution was used without application of intracanal medication. Counts of colony-forming units were carried out and the endotoxin was quantified by the chromogenic Limulus amobocyte lysate assay. The results were evaluated by analysis of variance and the Dunn test (5%). Root canal irrigation with propolis was effective to completely eliminate E. coli and reduce the amount of endotoxins. All intracanal medications contributed to the significant decrease in endotoxins. Only intracanal medications may reduce the amount of endotoxins in the root canals. The greatest efficacy was observed for medications containing Ca(OH)(2).
authors.affiliation: Department of Restorative Dentistry, School of Dentistry of São José dos Campos, São Paulo State University, São José dos Campos, Brazil. marcia@fosjc.unesp.br
authors.forename: Marcia Carneiro
authors.initials: MC
authors.lastname: Valera
authors.forename: Jucely Aparecida
authors.initials: JA
authors.lastname: da Rosa
authors.forename: Lilian Eiko
authors.initials: LE
authors.lastname: Maekawa
authors.forename: Luciane Dias
authors.initials: LD
authors.lastname: de Oliveira
authors.forename: Cláudio Antonio Talge
authors.initials: CA
authors.lastname: Carvalho
authors.forename: Cristiane Yumi
authors.initials: CY
authors.lastname: Koga-Ito
authors.forename: Antonio Olavo Cardoso
authors.initials: AO
authors.lastname: Jorge
mesh_terms: D000704:Analysis of Variance; D000890:Anti-Infective Agents / Q000494:pharmacology*; D002126:Calcium Hydroxide / Q000494:pharmacology; D002710:Chlorhexidine / Q000494:pharmacology; D015169:Colony Count, Microbial; D003786:Dental Pulp Cavity / Q000382:microbiology*; D004338:Drug Combinations; D004731:Endotoxins / Q000032:analysis / Q000037:antagonists & inhibitors; D004926:Escherichia coli / Q000187:drug effects*; D006801:Humans; D008033:Limulus Test; D011112:Polymyxin B / Q000494:pharmacology; D011429:Propolis / Q000494:pharmacology*; D012388:Root Canal Irrigants / Q000494:pharmacology*
chemical_list: D000890:Anti-Infective Agents; D004338:Drug Combinations; D004731:Endotoxins; D012388:Root Canal Irrigants; D011429:Propolis; D011112:Polymyxin B; D002126:Calcium Hydroxide; D002710:Chlorhexidine