The use of hybrid phage displaying antigen epitope and recombinant protein in the diagnosis of systemic Candida albicans infection in rabbits and cancer patients.
Hsp90 and Sap2 are 2 immunodominant antigens of Candida albicans. Both of them can induce the production of antibody. In this article, systemically infected rabbits were used to study the Hsp90 and Sap2 antibody production. Also, pET28a-Hsp90 protein, pET28a-Sap2 protein, hybrid phage displaying LKVIRK epitope, and hybrid phage displaying VKYTS epitope were used for diagnosis of the antibody in cancer patients. The results showed that the Sap2 antibody appeared earlier than Hsp90 antibody in systemically infected rabbits. Meanwhile, both of the antibodies can perform protection in rabbits. The conclusion is that Sap2 antibody, which appears at early stage in systemic candidiasis, may be better than Hsp90 antibody for the diagnosis of invasive candidiasis. For 141 sera of cancer patients, 52 sera were detected Sap2 antibody and 57 sera were detected Hsp90 antibody. Only 14 sera contained both the 2 antibodies. Although recombinant protein was slightly more sensitive than hybrid phage, there was no significant difference between them. For its easy preparation, less expensive hybrid phage displaying antigen epitope may be a better agent for diagnosis of candidiasis.
Quanping
Su
S
Institute of Genetics and Cytology, School of Life Sciences, Northeast Normal University, Changchun City, Jilin Province 130024, People's Republic of China.
Yanyan
Huai
H
Yicun
Wang
W
Zhigang
Ju
J
Yuling
Geng
G
Li
Wang
W
D000818:Animals; D000908:Antibodies, Fungal / Q000097:blood; D000946:Antigens, Fungal / Q000235:genetics / Q000276:immunology*; D016282:Aspartic Acid Endopeptidases / Q000235:genetics / Q000276:immunology; D002176:Candida albicans / Q000201:enzymology / Q000235:genetics / Q000276:immunology*; D058365:Candidiasis, Invasive / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology; D004195:Disease Models, Animal; D000939:Epitopes / Q000276:immunology*; D005656:Fungal Proteins / Q000235:genetics / Q000276:immunology; D018841:HSP90 Heat-Shock Proteins / Q000235:genetics / Q000276:immunology; D006801:Humans; D009369:Neoplasms / Q000150:complications / Q000276:immunology / Q000382:microbiology*; D019151:Peptide Library; D011817:Rabbits; D011994:Recombinant Proteins / Q000276:immunology*; D011995:Recombination, Genetic; D012680:Sensitivity and Specificity
D000908:Antibodies, Fungal; D000946:Antigens, Fungal; D000939:Epitopes; D005656:Fungal Proteins; D018841:HSP90 Heat-Shock Proteins; D019151:Peptide Library; D011994:Recombinant Proteins; D016282:Aspartic Acid Endopeptidases; C090753:SAP2 protein, Candida