Interaction of human T-cell lymphotropic virus type I Rex protein with Dicer suppresses RNAi silencing.
Double-stranded RNAs suppress the expression of homologous genes through an evolutionarily conserved process called RNA interference (RNAi) or post-transcriptional gene silencing. A bidentate nuclease called Dicer has been implicated as the protein responsible for the production of short interfering RNAs (siRNAs). In our experiments, Rex overexpression reduced the efficiency of short hairpin RNA (shRNA)-mediated RNAi. The interaction of Dicer with Rex inhibited the conversion of shRNA to siRNA. These results suggest that the interaction of Dicer with HTLV-I Rex inhibits Dicer activity and thereby reduces the efficiency of the conversion of shRNA to siRNA.
Abe
Makoto
M
Department of Life and Environmental Sciences, Chiba Institute of Technology, Narashino, Chiba, Japan.
Suzuki
Hitoshi
H
Nishitsuji
Hironori
H
Shida
Hisatoshi
H
Takaku
Hiroshi
H
D002460:Cell Line; D053487:DEAD-box RNA Helicases / Q000235:genetics / Q000378:metabolism*; D016370:Gene Products, rex / Q000235:genetics / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D008856:Microscopy, Fluorescence; D011485:Protein Binding; D034622:RNA Interference; D020871:RNA Stability; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism; D043244:Ribonuclease III / Q000235:genetics / Q000378:metabolism*; D014162:Transfection
D016370:Gene Products, rex; D034741:RNA, Small Interfering; D012367:RNA, Viral; C514844:rex Protein, Human T-lymphotropic virus 1; D049452:Green Fluorescent Proteins; D008156:Luciferases; C408087:DICER1 protein, human; D043244:Ribonuclease III; D053487:DEAD-box RNA Helicases