ABP1 mediates auxin inhibition of clathrin-dependent endocytosis in Arabidopsis.
Spatial distribution of the plant hormone auxin regulates multiple aspects of plant development. These self-regulating auxin gradients are established by the action of PIN auxin transporters, whose activity is regulated by their constitutive cycling between the plasma membrane and endosomes. Here, we show that auxin signaling by the auxin receptor AUXIN-BINDING PROTEIN 1 (ABP1) inhibits the clathrin-mediated internalization of PIN proteins. ABP1 acts as a positive factor in clathrin recruitment to the plasma membrane, thereby promoting endocytosis. Auxin binding to ABP1 interferes with this action and leads to the inhibition of clathrin-mediated endocytosis. Our study demonstrates that ABP1 mediates a nontranscriptional auxin signaling that regulates the evolutionarily conserved process of clathrin-mediated endocytosis and suggests that this signaling may be essential for the developmentally important feedback of auxin on its own transport.
Robert
Stéphanie
S
Department of Plant Systems Biology, VIB, 9052 Gent, Belgium.
Kleine-Vehn
Jürgen
J
Barbez
Elke
E
Sauer
Michael
M
Paciorek
Tomasz
T
Baster
Pawel
P
Vanneste
Steffen
S
Zhang
Jing
J
Simon
Sibu
S
Čovanová
Milada
M
Hayashi
Kenichiro
K
Dhonukshe
Pankaj
P
Yang
Zhenbiao
Z
Bednarek
Sebastian Y
SY
Jones
Alan M
AM
Luschnig
Christian
C
Aniento
Fernando
F
Zažímalová
Eva
E
Friml
Jiří
J
D017360:Arabidopsis / Q000166:cytology* / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D002966:Clathrin / Q000378:metabolism*; D004705:Endocytosis; D007210:Indoleacetic Acids / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism*
D029681:Arabidopsis Proteins; D002966:Clathrin; D007210:Indoleacetic Acids; D026901:Membrane Transport Proteins; D010940:Plant Proteins; D011956:Receptors, Cell Surface; C083846:auxin-binding protein 1