Ratiometric and intensity-based zinc sensors built on rhodol and rhodamine platforms.
A xanthene-forming condensation reaction yields rhodol and rhodamine dyes carrying a zinc-binding ligand that includes the aniline-type nitrogen donor of the fluorophores. Upon zinc coordination in neutral aqueous solution, rhodol RF3 behaves as a ratiometric sensor, and rhodamine RA1 acts as a turn-off intensity-based indicator. Both fluorescent compounds bind the divalent zinc cation with micromolar affinity.
Tomat
Elisa
E
Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
Lippard
Stephen J
SJ
D002264:Carboxylic Acids / Q000737:chemistry; D002623:Chemistry Techniques, Analytical / Q000295:instrumentation*; D000080027:Cyclams; D006571:Heterocyclic Compounds / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008024:Ligands; D009942:Organometallic Compounds / Q000737:chemistry*; D012235:Rhodamines / Q000737:chemistry*; D015032:Zinc / Q000032:analysis* / Q000737:chemistry
D002264:Carboxylic Acids; D000080027:Cyclams; D006571:Heterocyclic Compounds; D008024:Ligands; D009942:Organometallic Compounds; D012235:Rhodamines; C038072:cyclen; D015032:Zinc