Effect of time delay after necropsy on analysis of simian varicella-zoster virus expression in latently infected ganglia of rhesus macaques.
Studies of varicella-zoster virus gene expression during latency require the acquisition of human ganglia at autopsy. Concerns have been raised that the virus might reactivate immediately after death. Because features of varicella-zoster virus latency are similar in primate and human ganglia, we examined virus gene expression in tissues either processed immediately or kept at 4°C for 30 h before necropsy of two monkeys inoculated with simian varicella-zoster virus and euthanized 117 days later. Virus transcription and the detection of open reading frame (ORF) 63 protein in the cytoplasm of neurons were comparable. Thus, a 30-h delay after death did not affect varicella-zoster virus expression in latently infected ganglia.
Mahalingam
Ravi
R
Department of Neurology, University of Colorado School of Medicine, Aurora, Colorado 80045, USA. ravi.mahalingam@ucdenver.edu
Traina-Dorge
Vicki
V
Wellish
Mary
M
Deharo
Eileen
E
Golive
Anjani
A
Messaoudi
Ilhem
I
Gilden
Don
D
D000818:Animals; D002644:Chickenpox / Q000378:metabolism / Q000503:physiopathology*; D005724:Ganglia / Q000378:metabolism*; D015967:Gene Expression Regulation, Viral / Q000502:physiology*; D014645:Herpesvirus 3, Human / Q000378:metabolism / Q000502:physiology*; D017874:Immediate-Early Proteins / Q000378:metabolism*; D007150:Immunohistochemistry; D008253:Macaca mulatta; D009474:Neurons / Q000821:virology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D014759:Viral Envelope Proteins / Q000378:metabolism*; D014766:Viremia / Q000097:blood; D017735:Virus Latency / Q000502:physiology*
D017874:Immediate-Early Proteins; D014759:Viral Envelope Proteins; C074551:immediate early protein 63, Human herpesvirus 3