High-throughput screening for bioactive components from traditional Chinese medicine.
Throughout the centuries, traditional Chinese medicine has been a rich resource in the development of new drugs. Modern drug discovery, which relies increasingly on automated high throughput screening and quick hit-to-lead development, however, is confronted with the challenges of the chemical complexity associated with natural products. New technologies for biological screening as well as library building are in great demand in order to meet the requirements. Here we review the developments in these techniques under the perspective of their applicability in natural product drug discovery. Methods in library building, component characterizing, biological evaluation, and other screening methods including NMR and X-ray diffraction are discussed.
Zhu
Yanhui
Y
The National Center for Drug Screening the State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
Zhang
Zhiyun
Z
Zhang
Meng
M
Mais
Dale E
DE
Wang
Ming-Wei
MW
D000818:Animals; D001688:Biological Products / Q000737:chemistry* / Q000494:pharmacology*; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology; D057166:High-Throughput Screening Assays / Q000379:methods*; D006801:Humans; D008516:Medicine, Chinese Traditional; D010946:Plants, Medicinal / Q000737:chemistry
D001688:Biological Products; D004365:Drugs, Chinese Herbal