[The expression of toll-like receptor4 and NF-kappaB in ulcerative colitis].
OBJECTIVE
To investigate the expression of TLR4 and NF-kappaB in ulcerative colitis (UC).


METHODS
Colonic biopsy specimens were collected from active UC and controls. The expression of TLR4 and NF-kappaBp 65 were analyzed by immunohistochemistry (IHC) and RT-PCR.


RESULTS
RT-PCR revealed a significant increase of TLR4 and NF-kappaBp 65 antigen expression in colonic mucosa of UC compared with colonic mucosa of controls (TLR4: 143.658+/-33.870, 30.531+/-8.442, t=24.253, P<0.01; NF-kappaBp65: 185.773+/-37.625, 23.810+/-7.038, t=31.664, P<0.01). IHC show that the expression of TLR4 and NF-kappaB was significantly higher in colonic mucosa of UC compared with colonic mucosa of controls.


CONCLUSIONS
The expression of the TLR4 and NF-kappaB was increased in the colonic mucosa of UC compared with colonic mucosa of controls. It may be closely associated with the course of ulcerative colitis.
Yu
Zhen-hai
ZH
Department of Anatomy, Binzhou Medical College, Yantai Yu Huang Ding Hospital, Yantai, China. yt_whitewater@163.com
Chen
Li-dong
LD
Wang
Zhi-qiang
ZQ
Xu
Ning
N
D003093:Colitis, Ulcerative / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism; D051197:Toll-Like Receptor 4 / Q000235:genetics* / Q000378:metabolism; D051996:Transcription Factor RelA / Q000235:genetics* / Q000378:metabolism
C495345:TLR4 protein, human; D051197:Toll-Like Receptor 4; D051996:Transcription Factor RelA