Determination of pethidine in human plasma by LC-MS/MS.
A sensitive and selective liquid chromatography-tandem mass spectrometry method for the determination of pethidine in human plasma was developed and validated over the concentration range of 4-2000 ng/mL. After addition of ketamine as internal standard, liquid-liquid extraction was used to produce a protein-free extract. Chromatographic separation was achieved on a 100 × 2.1 mm, 5 µm particle, Allure™ PFP propyl column, with 45:40:15 (v/v/v) acetonitrile-methanol-water containing 0.2% formic acid as mobile phase. The MS data acquisition was accomplished by multiple reactions monitoring mode with positive electrospray ionization interface. The lower limit of quantification was 4 ng/mL; for inter-day and intra-day tests, the precision (RSD) for the entire validation was less than 7%, and the accuracy was within 95.9-106.5%. The method is sensitive and simple, and was successfully applied to analysis of samples of clinical intoxication.
Wang
Xianqin
X
Analytical and Testing Center, Wenzhou Medical College, Wenzhou 325035, China. lankywang@yahoo.cn
Xiang
Zheng
Z
Cai
Xiaojun
X
Wu
Haiya
H
Wang
Xuebao
X
Li
Junwei
J
Zhang
Meiling
M
D000328:Adult; D005591:Chemical Fractionation; D002853:Chromatography, Liquid / Q000379:methods*; D004355:Drug Stability; D005260:Female; D006801:Humans; D007223:Infant; D016014:Linear Models; D008297:Male; D008614:Meperidine / Q000097:blood*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
D008614:Meperidine