Mouse mammary tumor virus-like nucleotide sequences in canine and feline mammary tumors.
Mouse mammary tumor virus (MMTV) has been speculated to be involved in human breast cancer. Companion animals, dogs, and cats with intimate human contacts may contribute to the transmission of MMTV between mouse and human. The aim of this study was to detect MMTV-like nucleotide sequences in canine and feline mammary tumors by nested PCR. Results showed that the presence of MMTV-like env and LTR sequences in canine malignant mammary tumors was 3.49% (3/86) and 18.60% (16/86), respectively. For feline malignant mammary tumors, the presence of both env and LTR sequences was found to be 22.22% (2/9). Nevertheless, the MMTV-like LTR and env sequences also were detected in normal mammary glands of dogs and cats. In comparisons of the MMTV-like DNA sequences of our findings to those of NIH 3T3 (MMTV-positive murine cell line) and human breast cancer cells, the sequence similarities ranged from 94 to 98%. Phylogenetic analysis revealed that intermixing among sequences identified from tissues of different hosts, i.e., mouse, dog, cat, and human, indicated the MMTV-like DNA existing in these hosts. Moreover, the env transcript was detected in 1 of the 19 MMTV-positive samples by reverse transcription-PCR. Taken together, our study provides evidence for the existence and expression of MMTV-like sequences in neoplastic and normal mammary glands of dogs and cats.
Hsu
Wei-Li
WL
Graduate Institute of Microbiology and Public Health, Veterinary Medicine Teaching Hospital, College of Veterinary Medicine, National Chung Hsing University, 250, Kuo-Kuang Road, Taichung 40227, Taiwan.
Lin
Hsing-Yi
HY
Chiou
Shyan-Song
SS
Chang
Chao-Chin
CC
Wang
Szu-Pong
SP
Lin
Kuan-Hsun
KH
Chulakasian
Songkhla
S
Wong
Min-Liang
ML
Chang
Shih-Chieh
SC
D000818:Animals; D001483:Base Sequence; D002371:Cat Diseases / Q000821:virology*; D002415:Cats; D004283:Dog Diseases / Q000821:virology*; D004285:Dogs; D006801:Humans; D008321:Mammary Glands, Animal / Q000821:virology*; D015674:Mammary Neoplasms, Animal / Q000821:virology*; D008324:Mammary Tumor Virus, Mouse / Q000235:genetics / Q000302:isolation & purification*; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification*; D012689:Sequence Homology, Nucleic Acid; D020079:Terminal Repeat Sequences / Q000235:genetics; D014759:Viral Envelope Proteins / Q000235:genetics
D012367:RNA, Viral; D014759:Viral Envelope Proteins