Co-detection: ultra-reliable nanoparticle-based electrical detection of biomolecules in the presence of large background interference.
An ultra-reliable technique for detecting trace quantities of biomolecules is reported. The technique called "co-detection" exploits the non-linear redundancy amongst synthetically patterned biomolecular logic circuits for deciphering the presence or absence of target biomolecules in a sample. In this paper, we verify the "co-detection" principle on gold-nanoparticle-based conductimetric soft-logic circuits which use a silver-enhancement technique for signal amplification. Using co-detection, we have been able to demonstrate a great improvement in the reliability of detecting mouse IgG at concentration levels that are 10(5) lower than the concentration of rabbit IgG which serves as background interference.
Liu
Yang
Y
Department of Civil and Environmental Engineering, The University of Michigan, USA. yaliu@umich.edu
Gu
Ming
M
Alocilja
Evangelyn C
EC
Chakrabartty
Shantanu
S
D000818:Animals; D000888:Antibodies, Anti-Idiotypic; D055910:Antibodies, Immobilized; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods* / Q000706:statistics & numerical data; D055664:Electrochemical Techniques; D006046:Gold; D007074:Immunoglobulin G / Q000032:analysis; D053768:Metal Nanoparticles; D051379:Mice; D011817:Rabbits; D015203:Reproducibility of Results; D012834:Silver
D000888:Antibodies, Anti-Idiotypic; D055910:Antibodies, Immobilized; D007074:Immunoglobulin G; D012834:Silver; D006046:Gold