title: Dentate gyrus and hilus transection blocks seizure propagation and granule cell dispersion in a mouse model for mesial temporal lobe epilepsy.
abstract: Epilepsy-associated changes of the anatomical organization of the dentate gyrus and hilus may play a critical role in the initiation and propagation of seizures in mesial temporal lobe epilepsy (MTLE). This study evaluated the role of longitudinal projections in the propagation of hippocampal paroxysmal discharges (HPD) in dorsal hippocampus by performing a selective transection in a mouse model for MTLE obtained by a single unilateral intrahippocampal injection of kainic acid (KA). Full transections of the dentate gyrus and hilus were performed in the transverse axis at 22 days after KA injection when spontaneous HPD were fully developed. They: (i) significantly reduced the occurrence of HPD; (ii) increased their duration at the KA injection site; (iii) abolished their spread along the longitudinal axis of the hippocampal formation and; (iv) limited granule cell dispersion (GCD) of the dentate gyrus posterior to the transection. These data suggest that: (i) longitudinal projections through the dentate gyrus and hilus are involved in HPD spread; (ii) distant hippocampal circuits participate in the generation and cessation of HPD and; (iii) GCD requires continuous HPD to develop, even when seizures are established. Our data reveal a critical role for longitudinal projections in the generation and spread of hippocampal seizures.
authors.affiliation: Service de Neurochirurgie, Centre Hospitalier Sainte-Anne, Paris, France. johanpallud@hotmail.com
authors.forename: Johan
authors.initials: J
authors.lastname: Pallud
authors.forename: Ute
authors.initials: U
authors.lastname: Häussler
authors.forename: Mélanie
authors.initials: M
authors.lastname: Langlois
authors.forename: Sophie
authors.initials: S
authors.lastname: Hamelin
authors.forename: Bertrand
authors.initials: B
authors.lastname: Devaux
authors.forename: Colin
authors.initials: C
authors.lastname: Deransart
authors.forename: Antoine
authors.initials: A
authors.lastname: Depaulis
mesh_terms: D000818:Animals; D018891:Dentate Gyrus / Q000166:cytology / Q000503:physiopathology / Q000601:surgery; D004195:Disease Models, Animal; D004833:Epilepsy, Temporal Lobe / Q000473:pathology / Q000503:physiopathology* / Q000601:surgery*; D007608:Kainic Acid / Q000009:adverse effects; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D019599:Mossy Fibers, Hippocampal / Q000473:pathology / Q000503:physiopathology* / Q000601:surgery*; D009474:Neurons / Q000473:pathology*; D019635:Neurosurgical Procedures; D012640:Seizures / Q000139:chemically induced / Q000601:surgery
chemical_list: D007608:Kainic Acid