title: Design and validation of a highly discriminatory 10-locus Y-chromosome STR multiplex system.
abstract: The Y-chromosome STRs (short tandem repeat) markers are routinely utilized in the resolution of forensic casework related to sexual assault. For this, the forensic community has adopted a set of eleven (core) Y-STR that is incorporated in all commercial diagnostic systems. Our previous studies of Y-STR polymorphisms in the South African population identified low levels of diversity and discrimination capacity for many commercial marker sets, determining a limited applicability of these systems to the local population groups. To overcome this shortcoming, we designed a Y-STR 10-plex system that shows higher discriminatory capacity (DC) than available commercial systems. The markers were selected from a population group of 283 individuals with African, European and Asian ancestry genotyped at 45 Y-STRs, applying an optimization based selection procedure to achieve the highest possible DC with the minimal number of markers. The 10-plex was satisfactorily subjected to developmental validation tests following the SWGDAM guidelines and shows potential for its application to genealogical and evolutionary studies.
authors.affiliation: Forensic DNA Lab, University of the Western Cape, Biotechnology Department, Private Bag X17, 7535 Bellville, Cape Town, South Africa. medamato@uwc.ac.za
authors.forename: M E
authors.initials: ME
authors.lastname: D'Amato
authors.forename: V B
authors.initials: VB
authors.lastname: Bajic
authors.forename: S
authors.initials: S
authors.lastname: Davison
mesh_terms: D041322:Chromosomes, Human, Y; D016172:DNA Fingerprinting / Q000379:methods*; D017931:DNA Primers; D005819:Genetic Markers; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D044469:Racial Groups / Q000235:genetics; D013019:South Africa; D020080:Tandem Repeat Sequences
chemical_list: D017931:DNA Primers; D005819:Genetic Markers