title: Stemness evaluation of mesenchymal stem cells from placentas according to developmental stage: comparison to those from adult bone marrow.
abstract: This study was done to evaluate the stemness of human mesenchymal stem cells (hMSCs) derived from placenta according to the development stage and to compare the results to those from adult bone marrow (BM). Based on the source of hMSCs, three groups were defined: group I included term placentas, group II included first-trimester placentas, and group III included adult BM samples. The stemness was evaluated by the proliferation capacity, immunophenotypic expression, mesoderm differentiation, expression of pluripotency markers including telomerase activity. The cumulative population doubling, indicating the proliferation capacity, was significantly higher in group II (P<0.001, 31.7±5.8 vs. 15.7±6.2 with group I, 9.2±4.9 with group III). The pattern of immunophenotypic expression and mesoderm differentiation into adipocytes and osteocytes were similar in all three groups. The expression of pluripotency markers including ALP, SSEA-4, TRA-1-60, TRA-1-81, Oct-4, and telomerase were strongly positive in group II, but very faint positive in the other groups. In conclusions, hMSCs from placentas have different characteristics according to their developmental stage and express mesenchymal stemness potentials similar to those from adult human BMs.
authors.affiliation: Institute of Stem Cell Research, Korea University, Seoul, Korea.
authors.forename: Hwa Jung
authors.initials: HJ
authors.lastname: Sung
authors.forename: Soon Cheol
authors.initials: SC
authors.lastname: Hong
authors.forename: Ji Hyun
authors.initials: JH
authors.lastname: Yoo
authors.forename: Jee Hyun
authors.initials: JH
authors.lastname: Oh
authors.forename: Hye Jin
authors.initials: HJ
authors.lastname: Shin
authors.forename: In Young
authors.initials: IY
authors.lastname: Choi
authors.forename: Ki Hoon
authors.initials: KH
authors.lastname: Ahn
authors.forename: Sun Haeng
authors.initials: SH
authors.lastname: Kim
authors.forename: Yong
authors.initials: Y
authors.lastname: Park
authors.forename: Byung Soo
authors.initials: BS
authors.lastname: Kim
mesh_terms: D000954:Antigens, Surface / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology* / Q000378:metabolism; D049109:Cell Proliferation; D005260:Female; D006801:Humans; D016130:Immunophenotyping; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D008648:Mesoderm / Q000166:cytology; D050814:Octamer Transcription Factor-3 / Q000378:metabolism; D010920:Placenta / Q000166:cytology*; D010929:Placentation; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D011509:Proteoglycans / Q000378:metabolism; D055746:Stage-Specific Embryonic Antigens / Q000378:metabolism; D019098:Telomerase / Q000378:metabolism
chemical_list: D000954:Antigens, Surface; D050814:Octamer Transcription Factor-3; D011509:Proteoglycans; D055746:Stage-Specific Embryonic Antigens; C107064:TRA-1-60 antigen, human; C519803:TRA-1-81 antigen, human; C066584:stage-specific embryonic antigen-4; D019098:Telomerase