title: Molecules and signaling pathways involved in the expression of OC-STAMP during osteoclastogenesis.
abstract: The receptor activator of nuclear factor-κB ligand (RANKL) is a key factor in regulating osteoclastogenesis and in maintaining the survival of mature osteoclasts. We screened differentially expressed genes in RAW264.7 cells in response to RANKL and found osteoclast stimulatory transmembrane protein (OC-STAMP) as one of the RANKL-induced genes of interest. Recently, OC-STAMP has been identified as the RANKL-induced protein that promotes osteoclast differentiation, but the mechanism that regulates its expression is not understood. Therefore, the tissue distribution of OC-STAMP and the signaling pathways that regulate its expression were studied here. Similar to osteoclasts, OC-STAMP was expressed in most tissues, suggesting its involvement in the function of other tissues. Interestingly, OC-STAMP was downregulated by 17β-estradiol at high concentrations, suggesting the potential relationship between OC-STAMP and estrogen. Importantly, the knockdown of OC-STAMP at the transcript level resulted in the inhibition of multinucleated osteoclast formation and the decreased expression of genes including transcription factor (such as c-Jun), receptors (such as RANK and c-Fms), a signaling molecule (such as TRAF6), and a cell fusion-related molecule (such as meltrin-α), suggesting that the osteoclast differentiation needs the coordinated expression of OC-STAMP with several molecules required for transcription, signaling transduction, and cell fusion. Additionally, the treatment of its specific antibody inhibited the formation and bone resorptive activity of mature osteoclasts, suggesting its involvement in the function of mature osteoclasts. Furthermore, studies with pharmacological inhibitors suggested PKCβ or Akt might be the major signaling molecules to regulate the expression of OC-STAMP during osteoclastogenesis.
authors.affiliation: Laboratory of Chemical Genomics, Pharmacology Research Center, Korea Research Institute of Chemical Technology, Yuseong-gu, Daejeon, Korea.
authors.forename: Myung Hee
authors.initials: MH
authors.lastname: Kim
authors.forename: Mikyung
authors.initials: M
authors.lastname: Park
authors.forename: Seung-Hwa
authors.initials: SH
authors.lastname: Baek
authors.forename: Hye Joo
authors.initials: HJ
authors.lastname: Kim
authors.forename: Seong Hwan
authors.initials: SH
authors.lastname: Kim
mesh_terms: D051722:ADAM Proteins / Q000235:genetics; D000072199:ADAM12 Protein; D000818:Animals; D002478:Cells, Cultured; D020869:Gene Expression Profiling; D008565:Membrane Proteins / Q000235:genetics*; D051379:Mice; D010010:Osteoclasts / Q000166:cytology* / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D016755:Proto-Oncogene Proteins c-jun / Q000235:genetics; D053246:Receptor Activator of Nuclear Factor-kappa B; D016186:Receptor, Macrophage Colony-Stimulating Factor / Q000235:genetics; D015398:Signal Transduction / Q000235:genetics; D048029:TNF Receptor-Associated Factor 6 / Q000235:genetics
chemical_list: D008565:Membrane Proteins; C527519:OC-STAMP protein, mouse; D016755:Proto-Oncogene Proteins c-jun; D053246:Receptor Activator of Nuclear Factor-kappa B; D048029:TNF Receptor-Associated Factor 6; C505687:Tnfrsf11a protein, mouse; D016186:Receptor, Macrophage Colony-Stimulating Factor; D051722:ADAM Proteins; D000072199:ADAM12 Protein; C096115:Adam12 protein, mouse