Investigations on the interactions of aurintricarboxylic acid with bovine serum albumin: Steady state/time resolved spectroscopic and docking studies.
In this paper, the nature of the interactions between bovine serum albumin (BSA) and aurintricarboxylic acid (ATA) has been investigated by measuring steady state and time-resolved fluorescence, circular dichroism (CD), FT-IR and fluorescence anisotropy in protein environment under physiological conditions. From the analysis of the steady state and time-resolved fluorescence quenching of BSA in aqueous solution in presence of ATA it has been inferred that the nature of the quenching originates from the combined effect of static and dynamic modes. From the determination of the thermodynamic parameters obtained from temperature-dependent changes in K(b) (binding constant) it was apparent that the combined effect of hydrophobic association and electrostatic attraction is responsible for the interaction of ATA with BSA. The effect of ATA on the conformation of BSA has been examined by analyzing CD spectrum. Though the observed results demonstrate some conformational changes in BSA in presence of ATA but the secondary structure of BSA, predominantly of α-helix, is found to retain its identity. Molecular docking of ATA with BSA also indicates that ATA docks through hydrophobic interaction.
Bardhan
Munmun
M
Department of Spectroscopy, Indian Association for the Cultivation of Science, Jadavpur, Kolkata 700 032, West Bengal, India.
Chowdhury
Joydeep
J
Ganguly
Tapan
T
D000818:Animals; D001312:Aurintricarboxylic Acid / Q000378:metabolism* / Q000494:pharmacology; D001665:Binding Sites; D002417:Cattle; D031541:Fluorescence Resonance Energy Transfer; D008958:Models, Molecular; D011485:Protein Binding / Q000187:drug effects; D011487:Protein Conformation / Q000187:drug effects; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism*; D013057:Spectrum Analysis; D013816:Thermodynamics; D013997:Time Factors; D014508:Urea / Q000494:pharmacology
D012710:Serum Albumin, Bovine; D001312:Aurintricarboxylic Acid; D014508:Urea