title: Novel single nucleotide polymorphism identification in interleukin-6 gene of Pakistani sheep.
abstract: The present study aimed to identify single-nucleotide polymorphism (SNP) in coding and non-coding regions of interleukin-6 (IL-6) gene of Pakistani sheep. The IL-6 gene of 205 animals from nine sheep breeds were sequenced for screening of SNP. Characterizing the IL-6 gene revealed thirteen SNP sites within the intronic region of IL-6 gene. The novel SNPs found in the present study can serve as genetic marker for association studies with susceptibility/resistance to parasite infection in sheep. This is first report of SNP polymorphism of IL-6 gene of Pakistani sheep.
authors.affiliation: Department of Animal Breeding and Genetics, University of Agriculture Faisalabad, Faisalabad, Pakistan.
authors.forename: Safdar
authors.initials: S
authors.lastname: Ali
authors.forename: Masroor Ellahi
authors.initials: ME
authors.lastname: Babar
authors.forename: A
authors.initials: A
authors.lastname: Hossain Farid
authors.forename: Pervez
authors.initials: P
authors.lastname: Akhtar
authors.forename: Ali Raza
authors.initials: AR
authors.lastname: Awan
mesh_terms: D000818:Animals; D001483:Base Sequence; D017124:Conserved Sequence / Q000235:genetics; D005787:Gene Frequency / Q000235:genetics; D005838:Genotype; D015850:Interleukin-6 / Q000235:genetics*; D008969:Molecular Sequence Data; D050505:Mutant Proteins / Q000235:genetics; D010154:Pakistan; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D016415:Sequence Alignment; D034561:Sheep, Domestic / Q000235:genetics*
chemical_list: D015850:Interleukin-6; D050505:Mutant Proteins