title: p16INK4a and low-grade cervical intraepithelial neoplasia. Diagnostic and therapeutic implications.
abstract: The objectives of this study were to evaluate the evolution of a LSIL associated with p16INK4a overexpression and on the basis of this association, identify patients who would benefit from immediate treatment rather than a later follow-up. Two hundred and forty-five cervical biopsies were studied: 199 (81.2%) were classified CIN 1, 18 (7.4%) CIN 2/3 while 28 (11.4%) were not pathological. Immunohistochemistry revealed that 22 of the 217 CIN samples (11%) were positive for the p16INK4a antigen. The results of the PCR-ELISA for the research and typing of the HPV in these 22 cases were: 14 (63.6%) HPV 16; three (13.6%) HPV 31; 2 (9%) HPV 33; one (4.6%) HPV 43; one (4.6%) HPV 45; one (4.6%) HPV 18. Colposcopic and histological tests performed at four- and eight-month follow-ups in these patients revealed worsening of the initial lesion. Hence, we conclude that immediate therapy would be of benefit in these patients.
authors.affiliation: Department of Surgical Sciences, University of L'Aquila, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Di Stefano
authors.forename: V
authors.initials: V
authors.lastname: Accurti
authors.forename: G
authors.initials: G
authors.lastname: Coletti
authors.forename: A
authors.initials: A
authors.lastname: D'Alfonso
authors.forename: G
authors.initials: G
authors.lastname: Carta
mesh_terms: D000328:Adult; D014408:Biomarkers, Tumor / Q000032:analysis; D019941:Cyclin-Dependent Kinase Inhibitor p16; D005260:Female; D006801:Humans; D009363:Neoplasm Proteins / Q000032:analysis*; D027383:Papillomaviridae / Q000302:isolation & purification; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis* / Q000628:therapy / Q000821:virology; D002578:Uterine Cervical Dysplasia / Q000175:diagnosis* / Q000628:therapy / Q000821:virology
chemical_list: D014408:Biomarkers, Tumor; C000614131:CDKN2A protein, human; D019941:Cyclin-Dependent Kinase Inhibitor p16; D009363:Neoplasm Proteins