Antagonistic functions of two stardust isoforms in Drosophila photoreceptor cells.
Membrane-associated guanylate kinases (MAGUKs) are scaffolding proteins that organize supramolecular protein complexes, thereby partitioning the plasma membrane into spatially and functionally distinct subdomains. Their modular organization is ideally suited to organize protein complexes with cell type- or stage-specific composition, or both. Often more than one MAGUK isoform is expressed by one gene in the same cell, yet very little is known about their individual in vivo functions. Here, we show that two isoforms of Drosophila stardust, Sdt-H (formerly called Sdt-B2) and Sdt-D, which differ in their N terminus, are expressed in adult photoreceptors. Both isoforms associate with Crumbs and PATJ, constituents of the conserved Crumbs-Stardust complex. However, they form distinct complexes, localized at the stalk, a restricted region of the apical plasma membrane. Strikingly, Sdt-H and Sdt-D have antagonistic functions. While Sdt-H overexpression increases stalk membrane length and prevents light-dependent retinal degeneration, Sdt-D overexpression reduces stalk length and enhances light-dependent retinal degeneration. These results suggest that a fine-tuned balance of different Crumbs complexes regulates photoreceptor homeostasis.
Bulgakova
Natalia A
NA
Max-Planck-Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany.
Rentsch
Michaela
M
Knust
Elisabeth
E
D000818:Animals; D030801:Animals, Genetically Modified; D015153:Blotting, Western; D015536:Down-Regulation; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D005123:Eye / Q000254:growth & development / Q000378:metabolism / Q000648:ultrastructure; D005136:Eye Proteins / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D051528:Guanylate Kinases / Q000235:genetics / Q000378:metabolism*; D047468:Immunoprecipitation; D008565:Membrane Proteins / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D046529:Microscopy, Electron, Transmission; D009154:Mutation; D017956:Photoreceptor Cells, Invertebrate / Q000378:metabolism* / Q000648:ultrastructure; D011485:Protein Binding; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D011493:Protein Kinase C / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
D029721:Drosophila Proteins; D005136:Eye Proteins; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; C501446:PATJ protein, Drosophila; D020033:Protein Isoforms; C064360:crb protein, Drosophila; C532584:Par-6 protein, Drosophila; D011493:Protein Kinase C; D051528:Guanylate Kinases; C444479:sdt protein, Drosophila