title: Accelerating glycolytic flux of Torulopsis glabrata CCTCC M202019 at high oxidoreduction potential created using potassium ferricyanide.
abstract: This study aimed to increase the glycolytic flux of the multivitamin auxotrophic yeast Torulopsis glabrata by redirecting NADH oxidation from oxidative phosphorylation to membrane-bound ferric reductase. We added potassium ferricyanide as electron acceptor to T. glabrata culture broth at 20% dissolved oxygen (DO) concentration, which resulted in: (1) decreases in the NADH content, NADH/NAD(+) ratio, and ATP level of 45.3%, 60.3%, and 15.2%, respectively; (2) high activities of the key glycolytic enzymes hexokinase, phosphofructokinase, and pyruvate kinase, as well as high expression levels of the genes encoding these enzymes; and (3) increases in the specific glucose consumption rate and pyruvate yield of T. glabrata was by 45.5% and 23.1%, respectively. Our results showed that membrane-bound ferric reductase offers an alternative and efficient NADH oxidation pathway at lower DO concentration, which increases the glycolytic flux of T. glabrata.
authors.affiliation: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Qin
authors.forename: Li-Ming
authors.initials: LM
authors.lastname: Liu
authors.forename: Chang-Hao
authors.initials: CH
authors.lastname: Li
authors.forename: Sha
authors.initials: S
authors.lastname: Xu
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
mesh_terms: D041221:Candida glabrata / Q000378:metabolism*; D038181:FMN Reductase / Q000737:chemistry / Q000378:metabolism; D005292:Ferricyanides / Q000737:chemistry*; D006019:Glycolysis; D009243:NAD / Q000737:chemistry / Q000378:metabolism; D010084:Oxidation-Reduction; D010100:Oxygen / Q000737:chemistry / Q000378:metabolism; D010766:Phosphorylation
chemical_list: D005292:Ferricyanides; D009243:NAD; D038181:FMN Reductase; C023903:ferric citrate iron reductase; D010100:Oxygen; C028033:potassium ferricyanide