N-Acetyltransferase-2 (NAT2) genotype frequency among Jordanian volunteers.
OBJECTIVE
To determine the frequency of major NAT2 alleles and genotypes among the Jordanian population.


METHODS
DNA samples from 150 healthy Jordanian volunteers were analyzed by polymerase chain reaction followed by restriction fragment length polymorphism assays (PCR-RFLP) to determine the frequency of four major alleles: NAT2*4, NAT2*5, NAT2*6 and NAT2*7.


RESULTS
The frequency (95% confidence interval) of NAT2*4, NAT2*5, NAT2*6 and NAT2*7 alleles was 0.247 (0.198 - 0.296), 0.373 (0.318 - 0.428), 0.347. 0.293 - 0.401) and 0.033 (0.013 - 0.053), respectively. The most prevalent genotypes are those which encode slow acetylation phenotype. Around 58.7%, 33.3% and 8% of volunteers carried the slow, the intermediate and the fast-encoding genotypes, respectively.


CONCLUSIONS
NAT2 genotype profile among Jordanians is similar to Caucasians. However, NAT2*6 allele is slightly high in comparison with Arab Middle Eastern populations.
Jarrar
Y B
YB
Departments of Pharmacology, University of Jordan, Amman, Jordan.
Ismail
S
S
Irshaid
Y M
YM
D000328:Adult; D000483:Alleles; D001191:Arylamine N-Acetyltransferase / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D007597:Jordan; D008297:Male; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D044465:White People
D001191:Arylamine N-Acetyltransferase; C478900:NAT2 protein, human