Stemness evaluation of mesenchymal stem cells from placentas according to developmental stage: comparison to those from adult bone marrow.
This study was done to evaluate the stemness of human mesenchymal stem cells (hMSCs) derived from placenta according to the development stage and to compare the results to those from adult bone marrow (BM). Based on the source of hMSCs, three groups were defined: group I included term placentas, group II included first-trimester placentas, and group III included adult BM samples. The stemness was evaluated by the proliferation capacity, immunophenotypic expression, mesoderm differentiation, expression of pluripotency markers including telomerase activity. The cumulative population doubling, indicating the proliferation capacity, was significantly higher in group II (P<0.001, 31.7±5.8 vs. 15.7±6.2 with group I, 9.2±4.9 with group III). The pattern of immunophenotypic expression and mesoderm differentiation into adipocytes and osteocytes were similar in all three groups. The expression of pluripotency markers including ALP, SSEA-4, TRA-1-60, TRA-1-81, Oct-4, and telomerase were strongly positive in group II, but very faint positive in the other groups. In conclusions, hMSCs from placentas have different characteristics according to their developmental stage and express mesenchymal stemness potentials similar to those from adult human BMs.
Sung
Hwa Jung
HJ
Institute of Stem Cell Research, Korea University, Seoul, Korea.
Hong
Soon Cheol
SC
Yoo
Ji Hyun
JH
Oh
Jee Hyun
JH
Shin
Hye Jin
HJ
Choi
In Young
IY
Ahn
Ki Hoon
KH
Kim
Sun Haeng
SH
Park
Yong
Y
Kim
Byung Soo
BS
D000954:Antigens, Surface / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology* / Q000378:metabolism; D049109:Cell Proliferation; D005260:Female; D006801:Humans; D016130:Immunophenotyping; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D008648:Mesoderm / Q000166:cytology; D050814:Octamer Transcription Factor-3 / Q000378:metabolism; D010920:Placenta / Q000166:cytology*; D010929:Placentation; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D011509:Proteoglycans / Q000378:metabolism; D055746:Stage-Specific Embryonic Antigens / Q000378:metabolism; D019098:Telomerase / Q000378:metabolism
D000954:Antigens, Surface; D050814:Octamer Transcription Factor-3; D011509:Proteoglycans; D055746:Stage-Specific Embryonic Antigens; C107064:TRA-1-60 antigen, human; C519803:TRA-1-81 antigen, human; C066584:stage-specific embryonic antigen-4; D019098:Telomerase