Preparation approaches of the coated capillaries with liposomes in capillary electrophoresis.
The use of liposomes as coating materials in capillary electrophoresis has recently emerged as an important and popular research area. There are three preparation methods that are commonly used for coating capillaries with liposomes, namely physical adsorption, avidin-biotin binding and covalent coupling. Herein, the three different coating methods were compared, and the liposome-coated capillaries prepared by these methods were evaluated by studying systematically their EOF characterization and performance (repeatability, reproducibility and lifetime). The amount of immobilized phospholipids and the interactions between liposome or phospholipid membrane and neutral compounds for the liposome-coated capillaries prepared by these methods were also investigated in detail. Finally, the merits and disadvantages for each coating method were reviewed.
Mei
Jie
J
College of Pharmacy, Wuhan University, Wuhan 430071, China.
Tian
Yan-Ping
YP
He
Wen
W
Xiao
Yu-Xiu
YX
Wei
Juan
J
Feng
Yu-Qi
YQ
D000327:Adsorption; D001360:Avidin / Q000737:chemistry / Q000378:metabolism; D001710:Biotin / Q000737:chemistry / Q000378:metabolism; D004355:Drug Stability; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D008081:Liposomes / Q000737:chemistry*; D010743:Phospholipids / Q000737:chemistry / Q000378:metabolism; D015203:Reproducibility of Results; D013256:Steroids / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism
D008081:Liposomes; D010743:Phospholipids; D013256:Steroids; D001360:Avidin; D001710:Biotin