MiR-146b-5p suppresses EGFR expression and reduces in vitro migration and invasion of glioma.
Human miR-146b-5p is located on chromosome 10q24.3. Loss of the 10q24-26 region is frequently observed in gliomas. Here, we report that miR-146b-5p suppresses expression of epidermal growth factor receptor (EGFR) in human glioblastoma cell lines. Introduction of miR-146b-5p decreases cell invasion, migration, and phosphorylation of protein kinase B (AKT). MiR-146b-5p suppresses translation of EGFR, and binds to the EGFR 3'-UTR. Furthermore, analysis of U87-MG laser-capture microdissected cells in tumor-bearing mice indicated that expression of miR-146b-5p was inversely correlated with distance from the tumor core. These findings suggest that reconstitution of miR-146b-5p may be useful for the treatment of this invasive tumor.
Katakowski
Mark
M
Department of Neurology, Henry Ford Hospital, Detroit, Michigan 48202, USA.
Zheng
Xuguang
X
Jiang
Feng
F
Rogers
Thomas
T
Szalad
Alexandra
A
Chopp
Michael
M
D000818:Animals; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D066246:ErbB Receptors / Q000096:biosynthesis* / Q000235:genetics; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D005910:Glioma / Q000235:genetics* / Q000473:pathology; D006801:Humans; D007834:Lasers; D051379:Mice; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics*; D042282:Microdissection; D009361:Neoplasm Invasiveness / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
C542542:MIRN146 microRNA, human; D035683:MicroRNAs; C512478:EGFR protein, human; D066246:ErbB Receptors