Effects of differently polarized microwave radiation on the microscopic structure of the nuclei in human fibroblasts.
To investigate the influence of microwave radiation on the human fibroblast nuclei, the effects of three variants of electromagnetic wave polarization, linear and left-handed and right-handed elliptically polarized, were examined. Experimental conditions were: frequency (f) 36.65 GHz, power density (P) at the surface of exposed object 1, 10, 30, and 100 µW/cm(2), exposure time 10 s. Human fibroblasts growing in a monolayer on a cover slide were exposed to microwave electromagnetic radiation. The layer of medium that covered cells during microwave exposure was about 1 mm thick. Cells were stained immediately after irradiation by 2% (w/v) orcein solution in 45% (w/v) acetic acid. Experiments were made at room temperature (25 °C), and control cell samples were processed in the same conditions. We assessed heterochromatin granule quantity (HGQ) at 600× magnification. Microwave irradiation at the intensity of 1 µW/cm(2) produced no effect, and irradiation at the intensities of 10 and 100 µW/cm(2) induced an increase in HGQ. More intense irradiation induced more chromatin condensation. The right-handed elliptically polarized radiation revealed more biological activity than the left-handed polarized one.
Shckorbatov
Yuriy G
YG
Kharkiv National University, pl. Svobody 4, Kharkiv 61077, Ukraine. Yury.G.Shckorbatov@univer.kharkov.ua
Pasiuga
Vladimir N
VN
Goncharuk
Elena I
EI
Petrenko
Tatiana Ph
TP
Grabina
Valentin A
VA
Kolchigin
Nicolay N
NN
Ivanchenko
Dmitry D
DD
Bykov
Victor N
VN
Dumin
Oleksandr M
OM
D002467:Cell Nucleus / Q000528:radiation effects* / Q000648:ultrastructure; D002478:Cells, Cultured; D005347:Fibroblasts / Q000528:radiation effects* / Q000648:ultrastructure; D006570:Heterochromatin / Q000528:radiation effects; D006801:Humans; D008872:Microwaves
D006570:Heterochromatin