title: [Microbiological investigation of endoscopes at Brest Hospital over a period from 2007 to 2009].
abstract: OBJECTIVE
  The aim of this study is to evaluate the quality of disinfection of endoscopes at Brest hospital over a period from 2007 to 2009.
  
  
  METHODS
  Retrospective study of microbiological investigations of endoscopes done at Brest hospital from 2007 to 2009. The interpretation of the microbiological investigations is based on the recommendations of the Comité technique national des infections nosocomiales et infections liées aux soins (CTINILS) of 2007.
  
  
  RESULTS
  Most of the controls realized over the period deal with gastroenterological endoscopes (63.4 %) and bronchial endoscopes (21.8 %). Most of the controls (66.8 %) are conformed to the target level. Only 26.7 % of the controls get the level of action. Globally, the rate of level of action significantly increases (p=0.004) from 2007 (21.2 %) to 2009 (35.6 %). This increase is relatively important in gastroenterology endoscopy (46.8 % in 2009 versus 24.1 % in 2007) whereas the rate decreases in bronchial endoscopy (14.8 % in 2009 versus 25.9 % in 2007). In gastroenterological endoscopy, rates vary with the type of endoscopes and the context of controls, but there is no significant difference between manual disinfection and automated disinfection. The most frequent germ found in gastroenterological and bronchial endoscopies is Pseudomonas aeruginosa.
  
  
  CONCLUSIONS
  Our results show that it is very difficult to insure a perfect disinfection of endoscopes. Difficulties met are certainly related with the complexity of the endoscopes and of the techniques of disinfection. Infections of patients are very infrequent in endoscopy, which takes the question of the pertinence of the threshold used for microbiological investigations.
authors.affiliation: Service de santé publique, évaluation et hygiène hospitalière, CHRU Morvan, 2, avenue Foch, 29609 Brest cedex, France. philippe.saliou@chu-brest.fr
authors.forename: P
authors.initials: P
authors.lastname: Saliou
authors.forename: R
authors.initials: R
authors.lastname: Garlantézec
authors.forename: R
authors.initials: R
authors.lastname: Baron
authors.forename: J-J
authors.initials: JJ
authors.lastname: Quiot
authors.forename: F
authors.initials: F
authors.lastname: Cholet
authors.forename: M-F
authors.initials: MF
authors.lastname: Le Floch
authors.forename: B
authors.initials: B
authors.lastname: Lejeune
mesh_terms: D001331:Automation; D058491:Bacterial Load; D020680:Bronchoscopes / Q000382:microbiology; D004203:Disinfection / Q000379:methods / Q000592:standards; D019723:Endoscopes / Q000382:microbiology*; D020776:Endoscopes, Gastrointestinal / Q000382:microbiology; D004755:Enterobacteriaceae / Q000302:isolation & purification; D004866:Equipment Contamination / Q000517:prevention & control / Q000706:statistics & numerical data*; D005602:France; D019983:Guideline Adherence; D006785:Hospitals, University / Q000706:statistics & numerical data*; D017410:Practice Guidelines as Topic; D011550:Pseudomonas aeruginosa / Q000302:isolation & purification; D012189:Retrospective Studies; D012494:Sampling Studies