title: Evaluation of biological and biochemical quality of whey protein.
abstract: Nutritional and biochemical properties of noncommercial whey protein have been described since 1950. However, comparisons between commercial whey protein for human consumption and casein are rarely found. The aim of this study was to compare biological quality of a commercial whey protein with casein and its effect on biochemical parameters of rats. Thirty-two weanling Fisher rats were divided into three groups and given the following diets: casein group, standard diet (AOAC); whey protein group, modified AOAC diet with whey protein instead of casein; and casein:whey group, modified AOAC diet with 70%:30% casein:whey. A protein-free group was used for determination of endogenous nitrogen losses. Net protein ratio, protein efficiency ratio, and true digestibility were determined, and blood was collected for biochemical analysis. When compared with casein, whey protein showed significant differences for all biological parameters evaluated, as well as for albumin, total protein, total cholesterol, and glucose concentrations. Replacing 30% of casein with whey protein did not affect these parameters. A positive relation among whey protein, high-density lipoprotein-cholesterol, and paraoxonase activity was found. Hepatic or renal dysfunctions were not observed. In conclusion, in comparison with casein, commercial whey protein had higher values of biological parameters, and biochemical evaluation revealed it improved glycemic homeostasis, lipid status, and paraoxonase activity in rats.
authors.affiliation: Research Program in Biological Sciences, Institute of Exact and Biological Sciences, Federal University of Ouro Preto, Ouro Preto, Minas Gerais, Brazil. fabianokenji@gmail.com
authors.forename: Fabiano Kenji
authors.initials: FK
authors.lastname: Haraguchi
authors.forename: Maria Lucia
authors.initials: ML
authors.lastname: Pedrosa
authors.forename: Heberth de
authors.initials: Hd
authors.lastname: Paula
authors.forename: Rinaldo Cardoso dos
authors.initials: RC
authors.lastname: Santos
authors.forename: Marcelo Eustáquio
authors.initials: ME
authors.lastname: Silva
mesh_terms: D000818:Animals; D043303:Aryldialkylphosphatase / Q000097:blood; D001786:Blood Glucose / Q000032:analysis; D001798:Blood Proteins / Q000032:analysis; D002364:Caseins / Q000008:administration & dosage / Q000009:adverse effects / Q000378:metabolism*; D002784:Cholesterol / Q000097:blood; D019587:Dietary Supplements / Q000009:adverse effects; D004063:Digestion; D005243:Feces / Q000737:chemistry; D005527:Food, Fortified / Q000009:adverse effects; D048550:Hepatic Insufficiency / Q000097:blood; D006943:Hyperglycemia / Q000097:blood; D006949:Hyperlipidemias / Q000097:blood; D007408:Intestinal Absorption; D008297:Male; D008894:Milk Proteins / Q000008:administration & dosage / Q000009:adverse effects / Q000378:metabolism*; D009753:Nutritive Value; D051381:Rats; D011916:Rats, Inbred F344; D051437:Renal Insufficiency / Q000097:blood; D014886:Weaning; D015430:Weight Gain; D000067816:Whey Proteins
chemical_list: D001786:Blood Glucose; D001798:Blood Proteins; D002364:Caseins; D008894:Milk Proteins; D000067816:Whey Proteins; D002784:Cholesterol; D043303:Aryldialkylphosphatase