title: A family of plasmodesmal proteins with receptor-like properties for plant viral movement proteins.
abstract: Plasmodesmata (PD) are essential but poorly understood structures in plant cell walls that provide symplastic continuity and intercellular communication pathways between adjacent cells and thus play fundamental roles in development and pathogenesis. Viruses encode movement proteins (MPs) that modify these tightly regulated pores to facilitate their spread from cell to cell. The most striking of these modifications is observed for groups of viruses whose MPs form tubules that assemble in PDs and through which virions are transported to neighbouring cells. The nature of the molecular interactions between viral MPs and PD components and their role in viral movement has remained essentially unknown. Here, we show that the family of PD-located proteins (PDLPs) promotes the movement of viruses that use tubule-guided movement by interacting redundantly with tubule-forming MPs within PDs. Genetic disruption of this interaction leads to reduced tubule formation, delayed infection and attenuated symptoms. Our results implicate PDLPs as PD proteins with receptor-like properties involved the assembly of viral MPs into tubules to promote viral movement.
authors.affiliation: Institut de Biologie Moléculaire des Plantes du CNRS, Université de Strasbourg, Strasbourg, France.
authors.forename: Khalid
authors.initials: K
authors.lastname: Amari
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Boutant
authors.forename: Christina
authors.initials: C
authors.lastname: Hofmann
authors.forename: Corinne
authors.initials: C
authors.lastname: Schmitt-Keichinger
authors.forename: Lourdes
authors.initials: L
authors.lastname: Fernandez-Calvino
authors.forename: Pascal
authors.initials: P
authors.lastname: Didier
authors.forename: Alexander
authors.initials: A
authors.lastname: Lerich
authors.forename: Jérome
authors.initials: J
authors.lastname: Mutterer
authors.forename: Carole L
authors.initials: CL
authors.lastname: Thomas
authors.forename: Manfred
authors.initials: M
authors.lastname: Heinlein
authors.forename: Yves
authors.initials: Y
authors.lastname: Mély
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Maule
authors.forename: Christophe
authors.initials: C
authors.lastname: Ritzenthaler
mesh_terms: D017360:Arabidopsis / Q000254:growth & development / Q000378:metabolism / Q000821:virology; D002450:Cell Communication; D002473:Cell Wall / Q000378:metabolism; D027465:Chenopodium quinoa / Q000254:growth & development / Q000378:metabolism / Q000821:virology; D015151:Immunoblotting; D010935:Plant Diseases / Q000821:virology*; D018515:Plant Leaves / Q000254:growth & development / Q000378:metabolism / Q000821:virology; D053830:Plant Viral Movement Proteins / Q000378:metabolism*; D010942:Plant Viruses / Q000502:physiology*; D031425:Plasmodesmata / Q000378:metabolism* / Q000821:virology*; D021381:Protein Transport; D012367:RNA, Viral / Q000235:genetics; D011956:Receptors, Cell Surface / Q000378:metabolism*; D014026:Nicotiana / Q000254:growth & development / Q000378:metabolism / Q000821:virology
chemical_list: D053830:Plant Viral Movement Proteins; D012367:RNA, Viral; D011956:Receptors, Cell Surface