[An HGPRT defective mouse lung cancer cell line and its biological characteristics.].
BACKGROUND
To provide antigenic cells suitable for cell fusion to preparing lung cancer vaccine.


METHODS
An HGPRT (hypoxanthine guanidine phosphoribosyl transferase)-defective cell line , ALA9702 , was gradually induced by 8-azaguanine (8-AG) from mouse lung cancer cell line , LA795 . The biological characteristics and carcinogenicity of ALA9702 cells have been appraised both in vivo and in vitro.


RESULTS
ALA9702 cells have been cultured over one year in medium containing 20mug/ml 8-AG, and maintained their biologic characteristics and carcinogenicity of the original cells , LA795 cells.


CONCLUSIONS
ALA9702 cell line presents as stabile HGPRT-defective mouse lung cancer cell. It will provide useful antigenic cells for establishment of effective specific lung cancer vaccine therapy.
Lin
P
P
Cancer Center and Cancer Institute of The First University Hospital , West China University of Medical Sciences , Chengdu 610041 , P. R. China.
Lu
Y
Y
Zhou
H
H
Zhang
J
J
Huang
X
X