title: Immunoliposomal systems targeting primary effusion lymphoma: in vitro study.
abstract: OBJECTIVE
  To develop an appropriate liposomal formulation for gene delivery against primary effusion lymphoma (PEL), a herpesvirus HHV8-associated B-cell lymphoma.
  
  
  METHODS
  Cationic, cationic PEGylated and cationic PEGylated anti-CD138 liposomes (ILp) linking a monoclonal antibody expressed on PEL cells were prepared by a thin layer evaporation method, followed by extrusion technique. The formulations were mixed with a model oligonucleotide to form the lipoplexes and tested on a BCBL-1 cell (a PEL cell line). The transfection efficiency was evaluated by flow cytometry and confocal laser scanning microscopy analysis.
  
  
  RESULTS
  Based on antigen-antibody interaction, ILp mediated a specific gene delivery as shown by a significant increase in the transfection rate and a localized internalization of the oligonucleotide, in comparison with cationic liposomes and cationic PEGylated liposomes.
  
  
  CONCLUSIONS
  ILp could be proposed as effective carriers for oligonucleotide transfer in BCBL-1 cells. In vitro experimental results encourage us to further test the in vivo therapeutic potential of ILp for specific delivery of antitumoral agents.
authors.affiliation: Department of Pharmaceutical Sciences, University of Modena & Reggio Emilia, Via Campi 183, Modena, Italy. barbara.ruozi@unimo.it
authors.forename: Barbara
authors.initials: B
authors.lastname: Ruozi
authors.forename: Giovanni
authors.initials: G
authors.lastname: Riva
authors.forename: Daniela
authors.initials: D
authors.lastname: Belletti
authors.forename: Giovanni
authors.initials: G
authors.lastname: Tosi
authors.forename: Patrizia
authors.initials: P
authors.lastname: Barozzi
authors.forename: Mario
authors.initials: M
authors.lastname: Luppi
authors.forename: Flavio
authors.initials: F
authors.lastname: Forni
authors.forename: Maria Angela
authors.initials: MA
authors.lastname: Vandelli
mesh_terms: D001483:Base Sequence; D045744:Cell Line, Tumor; D004587:Electrophoresis, Agar Gel; D005434:Flow Cytometry; D016650:Fluorescein-5-isothiocyanate; D006801:Humans; D066298:In Vitro Techniques; D008081:Liposomes; D054685:Lymphoma, Primary Effusion / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D018625:Microscopy, Atomic Force; D018613:Microscopy, Confocal
chemical_list: D008081:Liposomes; D016650:Fluorescein-5-isothiocyanate