title: Complete genome sequence of Crohn's disease-associated adherent-invasive E. coli strain LF82.
abstract: BACKGROUND
  Ileal lesions of Crohn's disease (CD) patients are abnormally colonized by pathogenic adherent-invasive Escherichia coli (AIEC) able to invade and to replicate within intestinal epithelial cells and macrophages.
  
  
  RESULTS
  We report here the complete genome sequence of E. coli LF82, the reference strain of adherent-invasive E. coli associated with ileal Crohn's disease. The LF82 genome of 4,881,487 bp total size contains a circular chromosome with a size of 4,773,108 bp and a plasmid of 108,379 bp. The analysis of predicted coding sequences (CDSs) within the LF82 flexible genome indicated that this genome is close to the avian pathogenic strain APEC_01, meningitis-associated strain S88 and urinary-isolated strain UTI89 with regards to flexible genome and single nucleotide polymorphisms in various virulence factors. Interestingly, we observed that strains LF82 and UTI89 adhered at a similar level to Intestine-407 cells and that like LF82, APEC_01 and UTI89 were highly invasive. However, A1EC strain LF82 had an intermediate killer phenotype compared to APEC-01 and UTI89 and the LF82 genome does not harbour most of specific virulence genes from ExPEC. LF82 genome has evolved from those of ExPEC B2 strains by the acquisition of Salmonella and Yersinia isolated or clustered genes or CDSs located on pLF82 plasmid and at various loci on the chromosome.
  
  
  CONCLUSIONS
  LF82 genome analysis indicated that a number of genes, gene clusters and pathoadaptative mutations which have been acquired may play a role in virulence of AIEC strain LF82.
authors.affiliation: Clermont Université, Université d'Auvergne, JE2526, INRA, USC-2018, Clermont-Ferrand, France.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Miquel
authors.forename: Eric
authors.initials: E
authors.lastname: Peyretaillade
authors.forename: Laurent
authors.initials: L
authors.lastname: Claret
authors.forename: Amélie
authors.initials: A
authors.lastname: de Vallée
authors.forename: Carole
authors.initials: C
authors.lastname: Dossat
authors.forename: Benoit
authors.initials: B
authors.lastname: Vacherie
authors.forename: El Hajji
authors.initials: el H
authors.lastname: Zineb
authors.forename: Beatrice
authors.initials: B
authors.lastname: Segurens
authors.forename: Valerie
authors.initials: V
authors.lastname: Barbe
authors.forename: Pierre
authors.initials: P
authors.lastname: Sauvanet
authors.forename: Christel
authors.initials: C
authors.lastname: Neut
authors.forename: Jean-Frédéric
authors.initials: JF
authors.lastname: Colombel
authors.forename: Claudine
authors.initials: C
authors.lastname: Medigue
authors.forename: Francisco J M
authors.initials: FJ
authors.lastname: Mojica
authors.forename: Pierre
authors.initials: P
authors.lastname: Peyret
authors.forename: Richard
authors.initials: R
authors.lastname: Bonnet
authors.forename: Arlette
authors.initials: A
authors.lastname: Darfeuille-Michaud
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion; D002460:Cell Line; D003424:Crohn Disease / Q000382:microbiology*; D004926:Escherichia coli / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity* / Q000502:physiology; D004927:Escherichia coli Infections / Q000382:microbiology*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D016680:Genome, Bacterial; D006801:Humans; D007413:Intestinal Mucosa / Q000382:microbiology; D008264:Macrophages / Q000382:microbiology; D051379:Mice; D008969:Molecular Sequence Data; D010802:Phylogeny; D014774:Virulence
chemical_list: D029968:Escherichia coli Proteins