title: ABP1 mediates auxin inhibition of clathrin-dependent endocytosis in Arabidopsis.
abstract: Spatial distribution of the plant hormone auxin regulates multiple aspects of plant development. These self-regulating auxin gradients are established by the action of PIN auxin transporters, whose activity is regulated by their constitutive cycling between the plasma membrane and endosomes. Here, we show that auxin signaling by the auxin receptor AUXIN-BINDING PROTEIN 1 (ABP1) inhibits the clathrin-mediated internalization of PIN proteins. ABP1 acts as a positive factor in clathrin recruitment to the plasma membrane, thereby promoting endocytosis. Auxin binding to ABP1 interferes with this action and leads to the inhibition of clathrin-mediated endocytosis. Our study demonstrates that ABP1 mediates a nontranscriptional auxin signaling that regulates the evolutionarily conserved process of clathrin-mediated endocytosis and suggests that this signaling may be essential for the developmentally important feedback of auxin on its own transport.
authors.affiliation: Department of Plant Systems Biology, VIB, 9052 Gent, Belgium.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Robert
authors.forename: Jürgen
authors.initials: J
authors.lastname: Kleine-Vehn
authors.forename: Elke
authors.initials: E
authors.lastname: Barbez
authors.forename: Michael
authors.initials: M
authors.lastname: Sauer
authors.forename: Tomasz
authors.initials: T
authors.lastname: Paciorek
authors.forename: Pawel
authors.initials: P
authors.lastname: Baster
authors.forename: Steffen
authors.initials: S
authors.lastname: Vanneste
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Sibu
authors.initials: S
authors.lastname: Simon
authors.forename: Milada
authors.initials: M
authors.lastname: Čovanová
authors.forename: Kenichiro
authors.initials: K
authors.lastname: Hayashi
authors.forename: Pankaj
authors.initials: P
authors.lastname: Dhonukshe
authors.forename: Zhenbiao
authors.initials: Z
authors.lastname: Yang
authors.forename: Sebastian Y
authors.initials: SY
authors.lastname: Bednarek
authors.forename: Alan M
authors.initials: AM
authors.lastname: Jones
authors.forename: Christian
authors.initials: C
authors.lastname: Luschnig
authors.forename: Fernando
authors.initials: F
authors.lastname: Aniento
authors.forename: Eva
authors.initials: E
authors.lastname: Zažímalová
authors.forename: Jiří
authors.initials: J
authors.lastname: Friml
mesh_terms: D017360:Arabidopsis / Q000166:cytology* / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D002966:Clathrin / Q000378:metabolism*; D004705:Endocytosis; D007210:Indoleacetic Acids / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D002966:Clathrin; D007210:Indoleacetic Acids; D026901:Membrane Transport Proteins; D010940:Plant Proteins; D011956:Receptors, Cell Surface; C083846:auxin-binding protein 1