title: Characterization of Mauritian cynomolgus macaque major histocompatibility complex class I haplotypes by high-resolution pyrosequencing.
abstract: Major histocompatibility complex (MHC) class I alleles of nonhuman primates have been associated with disease susceptibility, resistance, and resolution. Here, using high-resolution pyrosequencing, we characterized MHC class I transcripts expressed in Mauritian cynomolgus macaques (MCM), a nonhuman primate population with restricted MHC diversity. Using this approach, we identified 67 distinct MHC class I transcripts encoded by the seven most frequent MCM MHC class I haplotypes, 40 (60%) of which span the complete open reading frames. These results double the number of MHC class I sequences previously defined by cloning and Sanger sequencing of cDNA-PCR products and provide a rapid, high-throughput, and economical method for MHC characterization. Overall, this approach significantly expanded our knowledge of MCM haplotypes and will facilitate future studies on disease pathogenesis and protective cellular immunity.
authors.affiliation: Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, WI 53705, USA.
authors.forename: Melisa L
authors.initials: ML
authors.lastname: Budde
authors.forename: Roger W
authors.initials: RW
authors.lastname: Wiseman
authors.forename: Julie A
authors.initials: JA
authors.lastname: Karl
authors.forename: Bozena
authors.initials: B
authors.lastname: Hanczaruk
authors.forename: Birgitte B
authors.initials: BB
authors.lastname: Simen
authors.forename: David H
authors.initials: DH
authors.lastname: O'Connor
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D005805:Genes, MHC Class I; D006239:Haplotypes; D008252:Macaca fascicularis / Q000235:genetics* / Q000276:immunology*; D017422:Sequence Analysis, DNA