title: New approach for the study of mite reproduction: The first transcriptome analysis of a mite, Phytoseiulus persimilis (Acari: Phytoseiidae).
abstract: Many species of mites and ticks are of agricultural and medical importance. Much can be learned from the study of transcriptomes of acarines which can generate DNA-sequence information of potential target genes for the control of acarine pests. High throughput transcriptome sequencing can also yield sequences of genes critical during physiological processes poorly understood in acarines, i.e., the regulation of female reproduction in mites. The predatory mite, Phytoseiulus persimilis, was selected to conduct a transcriptome analysis using 454 pyrosequencing. The objective of this project was to obtain DNA-sequence information of expressed genes from P. persimilis with special interest in sequences corresponding to vitellogenin (Vg) and the vitellogenin receptor (VgR). These genes are critical to the understanding of vitellogenesis, and they will facilitate the study of the regulation of mite female reproduction. A total of 12,556 contiguous sequences (contigs) were assembled with an average size of 935bp. From these sequences, the putative translated peptides of 11 contigs were similar in amino acid sequences to other arthropod Vgs, while 6 were similar to VgRs. We selected some of these sequences to conduct stage-specific expression studies to further determine their function.
authors.affiliation: Department of Entomology, North Carolina State University, Raleigh, NC 27695-7647, United States.
authors.forename: Ana R
authors.initials: AR
authors.lastname: Cabrera
authors.forename: Kevin V
authors.initials: KV
authors.lastname: Donohue
authors.forename: Sayed M S
authors.initials: SM
authors.lastname: Khalil
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Scholl
authors.forename: Charles
authors.initials: C
authors.lastname: Opperman
authors.forename: Daniel E
authors.initials: DE
authors.lastname: Sonenshine
authors.forename: R Michael
authors.initials: RM
authors.lastname: Roe
mesh_terms: D000818:Animals; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods*; D005786:Gene Expression Regulation; D008297:Male; D008925:Mites / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D011506:Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D011506:Proteins