title: Isothermal detection of RNA with restriction endonucleases.
abstract: Herein, we demonstrate how to detect nucleic acids that do not contain restriction endonuclease recognition sites with restriction endonucleases. We show that the topology of DNA probes used in this detection strategy remarkably affects the efficiency of RNA/DNA detection.
authors.affiliation: Department of Chemistry and Biochemistry, University of Maryland, College Park, MD 20742, USA.
authors.forename: Lei
authors.initials: L
authors.lastname: Yan
authors.forename: Shizuka
authors.initials: S
authors.lastname: Nakayama
authors.forename: Saron
authors.initials: S
authors.lastname: Yitbarek
authors.forename: Isabel
authors.initials: I
authors.lastname: Greenfield
authors.forename: Herman O
authors.initials: HO
authors.lastname: Sintim
mesh_terms: D015342:DNA Probes / Q000737:chemistry; D004262:DNA Restriction Enzymes / Q000737:chemistry* / Q000378:metabolism; D012313:RNA / Q000032:analysis*; D013696:Temperature
chemical_list: D015342:DNA Probes; D012313:RNA; D004262:DNA Restriction Enzymes