Nonomuraea rhizophila sp. nov., an actinomycete isolated from rhizosphere soil.
A novel actinomycete, designated strain YIM 67092(T), was isolated from rhizosphere soil of the perennial vine Tripterygium wilfordii Hook. f. collected from Yunnan province, South-west China. The strain formed well differentiated aerial and substrate mycelia and grew in the presence of up to 7 % (w/v) NaCl. Phylogenetic analysis based on the 16S rRNA gene showed that strain YIM 67092(T) belonged to the genus Nonomuraea, with highest sequence similarity to Nonomuraea rosea GW 12687(T) (99.0 %). Sequence similarities between strain YIM 67092(T) and other species of the genus Nonomuraea ranged from 97.8 % (Nonomuraea dietziae DSM 44320(T)) to 93.8 % (Nonomuraea kuesteri GW 14-1925(T)). Key morphological, physiological and chemotaxonomic characteristics of strain YIM 67092(T) were congruent with the description of the genus Nonomuraea. The G+C content of the genomic DNA was 69.3 mol%. Based on comparative analysis of physiological, biochemical and chemotaxonomic data, including low DNA-DNA hybridization results, strain YIM 67092(T) represents a novel species of the genus Nonomuraea, for which the name Nonomuraea rhizophila sp. nov. is proposed. The type strain is YIM 67092(T) ( = CCTCC AA 209044(T)  = DSM 45382(T)).
Zhao
Guo-Zhen
GZ
Key Laboratory for Microbial Diversity in Southwest China, Ministry of Education and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
Li
Jie
J
Key Laboratory of marine Bio-resources Sustainable Utilization CAS, RNAM Center for Marine Microbiology, Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, PR China.|Key Laboratory for Microbial Diversity in Southwest China, Ministry of Education and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
Huang
Hai-Yu
HY
Key Laboratory for Microbial Diversity in Southwest China, Ministry of Education and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
Zhu
Wen-Yong
WY
Key Laboratory for Microbial Diversity in Southwest China, Ministry of Education and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
Xu
Li-Hua
LH
Key Laboratory for Microbial Diversity in Southwest China, Ministry of Education and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
Li
Wen-Jun
WJ
Key Laboratory of marine Bio-resources Sustainable Utilization CAS, RNAM Center for Marine Microbiology, Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, PR China.|Key Laboratory for Microbial Diversity in Southwest China, Ministry of Education and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D058441:Rhizosphere; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism; D012988:Soil Microbiology; D029757:Tripterygium / Q000382:microbiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride