title: Cardiac oxidative stress and inflammation are similar in SAMP8 and SAMR1 mice and unaltered by curcumin and Ginkgo biloba extract intake.
abstract: Chronic inflammation and oxidative stress increase with advancing age and appear to be involved in the pathogenesis of coronary heart disease, the leading cause of death worldwide. There is a need for animal models that reflect the increases in pro-inflammatory cytokines and oxidative damage observed during aging in humans. We therefore aimed to investigate the suitability of the fast-aging senescence-accelerated mouse-prone 8 (SAMP8) strain and its normally aging control senescence-accelerated mouse-resistant 1 (SAMR1) to study the age-dependent changes in cytokines, oxidative damage and antioxidants in the heart. To this end, 2-months-old male SAMR1 and SAMP8 mice were fed a Western type diet (control groups) for 5 months. Two groups of SAMP8 mice were simultaneously fed identical diets fortified with 0.5 g curcumin or 1.0 g Ginkgo biloba extract EGb 761(®) per kg diet. Heart tissue homogenates were analysed for protein carbonyls, glutathione, glutathione disulfide, methionine, cysteine and uric acid as well as the cytokines tumor-necrosis factor-α, interleukin-1β, interleukin-6, and monocyte chemoattractant protein 1. Neither the strain (SAMR1 or SAMP8) nor antioxidant intake (curcumin or EGb 761(®)) affected the concentrations of the measured parameters. In conclusion, our data do not support the suitability of the SAMP8 and SAMR1 strains as a model to study age-related changes in pro-inflammatory cytokines and oxidative stress parameters in the heart.
authors.affiliation: Institute of Human Nutrition and Food Science, University of Kiel, Hermann-Rodewald-Str. 6, 24118 Kiel, Germany.
authors.forename: Christina
authors.initials: C
authors.lastname: Schiborr
authors.forename: Gunter P
authors.initials: GP
authors.lastname: Eckert
authors.forename: Jakob
authors.initials: J
authors.lastname: Weissenberger
authors.forename: Walter E
authors.initials: WE
authors.lastname: Müller
authors.forename: Dorothea
authors.initials: D
authors.lastname: Schwamm
authors.forename: Tilman
authors.initials: T
authors.lastname: Grune
authors.forename: Gerald
authors.initials: G
authors.lastname: Rimbach
authors.forename: Jan
authors.initials: J
authors.lastname: Frank
mesh_terms: D000367:Age Factors; D000375:Aging; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D050197:Atherosclerosis / Q000188:drug therapy* / Q000503:physiopathology; D003327:Coronary Disease / Q000188:drug therapy* / Q000503:physiopathology; D003474:Curcumin / Q000494:pharmacology*; D016207:Cytokines / Q000378:metabolism; D005260:Female; D020441:Ginkgo biloba; D006321:Heart / Q000187:drug effects; D006801:Humans; D007249:Inflammation / Q000188:drug therapy*; D053583:Interleukin-1beta / Q000378:metabolism; D015850:Interleukin-6; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D023421:Models, Animal; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D050050:Protein Carbonylation / Q000187:drug effects; D000096983:Ginkgo Extract
chemical_list: D000975:Antioxidants; D003474:Curcumin; D016207:Cytokines; D053583:Interleukin-1beta; D015850:Interleukin-6; D010936:Plant Extracts; C063170:Ginkgo biloba extract; D000096983:Ginkgo Extract