Sequence discrimination of the Zα domain of human ADAR1 during B-Z transition of DNA duplexes.
The Zα domain of human ADAR1 (Zα(ADAR1)) preferentially binds Z-DNA rather than B-DNA with high binding affinity. Zα(ADAR1) binds to the Z-conformation of both non-CG-repeat DNA duplexes and a d(CGCGCG)(2) duplex similarly. We performed NMR experiments on complexes between the Zα(ADAR1) and non-CG-repeat DNA duplexes, d(CACGTG)(2) or d(CGTACG)(2), with a variety of protein-DNA molar ratios. Comparison of these results with those from the analysis of d(CGCGCG)(2) in the previous study suggests that Zα(ADAR1) exhibits the sequence preference of d(CGCGCG)(2)≫d(CACGTG)(2)>d(CGTACG)(2) through multiple sequence discrimination steps during the B-Z transition.
Seo
Yeo-Jin
YJ
Department of Chemistry, RINS, Gyeongsang National University, Jinju, Gyengnam, Republic of Korea.
Ahn
Hee-Chul
HC
Lee
Eun-Hae
EH
Bang
Jongchul
J
Kang
Young-Min
YM
Kim
Hee-Eun
HE
Lee
Yeon-Mi
YM
Kim
Kyungmin
K
Choi
Byong-Seok
BS
Lee
Joon-Hwa
JH
D000243:Adenosine Deaminase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D000465:Algorithms; D001483:Base Sequence; D001665:Binding Sites; D001667:Binding, Competitive; D002151:Calorimetry / Q000379:methods; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D043542:DNA, Z-Form / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008956:Models, Chemical; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000737:chemistry / Q000378:metabolism; D016601:RNA-Binding Proteins
D043542:DNA, Z-Form; D009841:Oligonucleotides; D016601:RNA-Binding Proteins; D004247:DNA; C567955:ADARB1 protein, human; D000243:Adenosine Deaminase