title: Interferon-gamma +874 T/A and interleukin-10 -1082 A/G single nucleotide polymorphism in Egyptian children with tuberculosis.
abstract: The aim was to investigate the association of interferon-gamma (IFN-γ) +874 T/A and interleukin-10 (IL-10)-1082 A/G single nucleotide polymorphisms with tuberculous infection and post-BCG lymphadenitis in Egyptian children. IFN-γ +874 T/A and IL-10 -1082 A/G polymorphism detection by amplification refractory mutation system technique was carried out for 110 patients with TB, 40 patients with post-BCG lymphadenitis and 118 healthy controls. IFN-γ +874 A allele was higher in TB and post-BCG patients than those in healthy controls (Pc=0.006 and 0.002, respectively). IFN-γ +874 genotype AA was significantly higher in patients with TB than that in control (Pc=0.015), in extrapulmonary than patients with pulmonary TB (PTB) (Pc=0.009), and young children with TB below 5 years (Pc=0.024). No statistically significant differences were observed between patients with TB and controls for the frequency of IL-10(-1082) alleles or genotypes (P>0.05); however, a statistically significant difference in the frequency of IL-10 (-1082) GG genotype was found between patients with pulmonary and extrapulmonary TB (Pc=0.003). Low producer IFN-γ +874 A/A genotype is associated with post-BCG lymphadenitis and TB disease especially in younger children below 5 years. IL-10-1082 G/G genotype did not exhibit significant association except for increased GG frequency in PTB. Both cytokine polymorphisms have no relation to tuberculin reaction in patients with TB.
authors.affiliation: Clinical Immunology Unit, Clinical Pathology Department, Mansoura Faculty of Medicine, Mansoura, Egypt. youssefmosaad@yahoo.com
authors.forename: Y M
authors.initials: YM
authors.lastname: Mosaad
authors.forename: O E
authors.initials: OE
authors.lastname: Soliman
authors.forename: Z E
authors.initials: ZE
authors.lastname: Tawhid
authors.forename: D M
authors.initials: DM
authors.lastname: Sherif
mesh_terms: D000293:Adolescent; D001500:BCG Vaccine / Q000008:administration & dosage / Q000009:adverse effects / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D004534:Egypt; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D007223:Infant; D007371:Interferon-gamma / Q000235:genetics*; D016753:Interleukin-10 / Q000235:genetics*; D008199:Lymphadenitis / Q000139:chemically induced / Q000276:immunology; D008297:Male; D016017:Odds Ratio; D020641:Polymorphism, Single Nucleotide; D014376:Tuberculosis / Q000235:genetics* / Q000276:immunology / Q000517:prevention & control; D014397:Tuberculosis, Pulmonary / Q000235:genetics / Q000276:immunology / Q000517:prevention & control
chemical_list: D001500:BCG Vaccine; D016753:Interleukin-10; D007371:Interferon-gamma