title: Assembly of filopodia by the formin FRL2 (FMNL3).
abstract: Actin-dependent finger-like protrusions such as filopodia and microvilli are widespread in eukaryotes, but their assembly mechanisms are poorly understood. Filopodia assembly requires at least three biochemical activities on actin: actin filament nucleation, prolonged actin filament elongation, and actin filament bundling. These activities are shared by several mammalian formin proteins, including mDia2, FRL1 (also called FMNL1), and FRL2 (FMNL3). In this paper, we compare the abilities of constructs from these three formins to induce filopodia. FH1-FH2 constructs of both FRL2 and mDia2 stimulate potent filopodia assembly in multiple cell types, and enrich strongly at filopodia tips. In contrast, FRL1 FH1-FH2 lacks this activity, despite possessing similar biochemical activities and being highly homologous to FRL2. Chimeric FH1-FH2 experiments between FRL1 and FRL2 show that, while both an FH1 and an FH2 are needed, either FH1 domain supports filopodia assembly but only FRL2's FH2 domain allows this activity. A mutation that compromises FRL2's barbed end binding ability abolishes filopodia assembly. FRL2's ability to stimulate filopodia assembly is not altered by additional domains (GBD, DID, DAD), but is significantly reduced in the full-length construct, suggesting that FRL2 is subject to inhibitory regulation. The data suggest that the FH2 domain of FRL2 possesses properties not shared by FRL1 that allow it to generate filopodia.
authors.affiliation: Department of Biology, Stanford University, Stanford, California, USA.
authors.forename: Elizabeth S
authors.initials: ES
authors.lastname: Harris
authors.forename: Timothy J
authors.initials: TJ
authors.lastname: Gauvin
authors.forename: Ernest G
authors.initials: EG
authors.lastname: Heimsath
authors.forename: Henry N
authors.initials: HN
authors.lastname: Higgs
mesh_terms: D008841:Actin Cytoskeleton / Q000737:chemistry / Q000378:metabolism*; D000199:Actins / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D000080865:Formins; D006367:HeLa Cells; D006801:Humans; D019169:Jurkat Cells; D051379:Mice; D008840:Microfilament Proteins / Q000737:chemistry / Q000378:metabolism; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D011554:Pseudopodia / Q000378:metabolism*; D041701:Swiss 3T3 Cells; D014162:Transfection
chemical_list: D000199:Actins; C476209:FMNL3 protein, human; D000080865:Formins; D008840:Microfilament Proteins; D011506:Proteins