Mass identification of transcriptional units expressed from the Bombyx mori nucleopolyhedrovirus genome.
In order to identify the transcriptional units expressed from an entire nucleopolyhedrovirus (NPV) genome during infection, we constructed a full-length-enriched cDNA library from Bombyx mori NPV (BmNPV)-infected BmN cells. We randomly sequenced 11,520 clones from both ends to obtain a total of 4679 BmNPV-derived transcriptional units. The data revealed a number of novel transcripts, including putative non-coding RNAs, most of which are expressed from recognized baculovirus early or late promoter motifs. These findings provide new insights into the complex transcriptional regulation of an NPV genome and suggest roles for as-yet-uncharacterized transcripts.
Katsuma
Susumu
S
Department of Agricultural and Environmental Biology, University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-8657, Japan. katsuma@ss.ab.a.u-tokyo.ac.jp
Kang
WonKyung
W
Shin-i
Tadasu
T
Ohishi
Kazuko
K
Kadota
Koji
K
Kohara
Yuji
Y
Shimada
Toru
T
D000818:Animals; D012831:Bombyx / Q000821:virology; D002460:Cell Line; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D020869:Gene Expression Profiling / Q000379:methods*; D015967:Gene Expression Regulation, Viral; D015723:Gene Library; D016679:Genome, Viral; D008969:Molecular Sequence Data; D017924:Nucleopolyhedroviruses / Q000235:genetics* / Q000254:growth & development*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D022661:RNA, Untranslated / Q000096:biosynthesis / Q000235:genetics; D012367:RNA, Viral / Q000096:biosynthesis / Q000235:genetics; D017422:Sequence Analysis, DNA
D004279:DNA, Viral; D012333:RNA, Messenger; D022661:RNA, Untranslated; D012367:RNA, Viral