title: Double-stranded RNA viral infection of Trichomonas vaginalis and correlation with genetic polymorphism of isolates.
abstract: Trichomonas vaginalis can be infected with double-stranded RNA (dsRNA) viruses known as T. vaginalis virus (TVV). This viral infection may have important implications for trichomonal virulence and disease pathogenesis. The objective of this study was to determine the possible correlation between the T. vaginalis genetic polymorphism and the isolate infection with TVV. The Random Amplified Polymorphic DNA (RAPD) technique was used to determine genetic differences among 37 isolates of T. vaginalis using a panel of 30 random primers and these genetic data were correlated with the infection of isolates with TVV. The trees drawn based on RAPD data showed significantly association with the presence of TVV (P = 0.028) demonstrating the existence of concordance between the genetic relatedness and the presence of TVV in T. vaginalis isolates. This result could point to a predisposition of T. vaginalis for the viral enters and/or survival.
authors.affiliation: Departamento de Parasitologia, Instituto de Medicina Tropical Pedro Kourí, Apartado Postal 601, Marianao 13, Ciudad de La Habana, Cuba. fraga@ipk.sld.cu
authors.forename: Jorge
authors.initials: J
authors.lastname: Fraga
authors.forename: Lazara
authors.initials: L
authors.lastname: Rojas
authors.forename: Idalia
authors.initials: I
authors.lastname: Sariego
authors.forename: Ayme
authors.initials: A
authors.lastname: Fernández-Calienes
mesh_terms: D000293:Adolescent; D005260:Female; D006801:Humans; D010802:Phylogeny; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique; D019196:Totiviridae / Q000502:physiology*; D014247:Trichomonas Vaginitis / Q000469:parasitology; D014246:Trichomonas vaginalis / Q000145:classification / Q000235:genetics* / Q000821:virology*