B cells help alloreactive T cells differentiate into memory T cells.
B cells are recognized as effector cells in allograft rejection that are dependent upon T cell help to produce alloantibodies causing graft injury. It is not known if B cells can also help T cells differentiate into memory cells in the alloimmune response. We found that in B-cell-deficient hosts, differentiation of alloreactive T cells into effectors was intact whereas their development into memory T cells was impaired. To test if B cell help for T cells was required for their continued differentiation into memory T cells, activated T cells were sorted from alloimmunized mice and transferred either with or without B cells into naïve adoptive hosts. Activated T cells cotransferred with B cells gave rise to more memory T cells than those transferred without B cells and upon recall, mediated accelerated rejection of skin allografts. Cotransfer of B cells led to increased memory T cells by enhancing activated CD4 T-cell proliferation and activated CD8 T-cell survival. These results indicate that B cells help alloreactive T-cell differentiation, proliferation and survival to generate optimal numbers of functional memory T cells.
Ng
Y-H
YH
Department of Medicine, Division of Nephrology, National University Hospital, Singapore.
Oberbarnscheidt
M H
MH
Chandramoorthy
H C K
HC
Hoffman
R
R
Chalasani
G
G
D019264:Adoptive Transfer; D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000502:physiology* / Q000637:transplantation; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology; D002452:Cell Count; D002454:Cell Differentiation / Q000276:immunology; D049109:Cell Proliferation; D002470:Cell Survival / Q000502:physiology; D006084:Graft Rejection / Q000209:etiology / Q000473:pathology; D007156:Immunologic Memory / Q000502:physiology; D007518:Isoantibodies / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D016038:Skin Transplantation; D013601:T-Lymphocytes / Q000166:cytology* / Q000276:immunology* / Q000637:transplantation; D013997:Time Factors; D014184:Transplantation, Homologous
D007518:Isoantibodies