Simultaneous HPLC analysis of triamcinolone acetonide and budesonide in microdialysate and rat plasma: application to a pharmacokinetic study.
A specific and reliable HPLC-PDA method for the quantitative determination of triamcinolone acetonide, budesonide and fluticasone propionate (as internal standards) in small volumes of microdialysate and rat plasma was developed. An efficient solid-phase extraction (SPE) procedure for plasma samples yielded extremely clean extracts with overall recovery of 104.3% and 95.7% for triamcinolone acetonide (TA) and fluticasone propionate, respectively. Plasma extracts obtained after SPE and microdialysis samples were directly injected on a C18 column to separation. The method has been validated with good linearity, sensitivity, specificity and high accuracy (RE -5.28% to 9.14%) and precision (CV 0.50% to 6.62%) on both matrices. In stability studies, TA and budesonide were stable during storage and assay procedures. The method was applied to a pharmacokinetic study in rodents using microdialysis to determine protein unbound TA concentrations in blood and muscle.
Vieira
Manuela de L T
Mde L
Department of Pharmaceutics, College of Pharmacy, University of Florida, Gainesville, FL 32610, USA.
Singh
Rajandra P
RP
Derendorf
Hartmut
H
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000097:blood* / Q000493:pharmacokinetics; D019819:Budesonide / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008297:Male; D017551:Microdialysis; D009132:Muscles / Q000737:chemistry; D051381:Rats; D052616:Solid Phase Extraction; D014222:Triamcinolone Acetonide / Q000097:blood* / Q000493:pharmacokinetics
D000893:Anti-Inflammatory Agents; D019819:Budesonide; D014222:Triamcinolone Acetonide