Defining the phosphite-regulated transcriptome of the plant pathogen Phytophthora cinnamomi.
Phosphite, an analog of phosphate is used to control oomycete diseases on a wide range of horticultural crops and in native ecosystems. In this study, we investigated morphological and transcriptional changes induced in Phytophthora cinnamomi by phosphite. Cytological observations revealed that phosphite caused hyphal distortions and lysis of cell walls and had an adverse effect on hyphal growth. At the molecular level, the expression levels of 43 transcripts were changed. Many of these encoded proteins involved in cell wall synthesis, or cytoskeleton functioning. The results of both the microscopic and molecular investigations are consistent with phosphite inhibiting the function of the cytoskeleton and cell wall synthesis.
King
Michaela
M
Centre for Phytophthora Science and Management, School of Biological Sciences and Biotechnology, Murdoch University, Perth, WA 6150, Australia.
Reeve
Wayne
W
Van der Hoek
Mark B
MB
Williams
Nari
N
McComb
Jen
J
O'Brien
Philip A
PA
Hardy
Giles E St J
GE
D001483:Base Sequence; D002473:Cell Wall / Q000187:drug effects / Q000378:metabolism; D003599:Cytoskeleton / Q000187:drug effects / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects; D020869:Gene Expression Profiling; D020411:Oligonucleotide Array Sequence Analysis; D017905:Phosphites / Q000494:pharmacology*; D010838:Phytophthora / Q000187:drug effects* / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D010935:Plant Diseases / Q000469:parasitology; D020133:Reverse Transcriptase Polymerase Chain Reaction
D017931:DNA Primers; D017905:Phosphites