Proteomic analysis of haptoglobin and amyloid A protein levels in patients with vivax malaria.
Advancements in the field of proteomics have provided great opportunities for the development of diagnostic and therapeutic tools against human diseases. In this study, we analyzed haptoglobin and amyloid A protein levels of vivax malaria patients with combinations of depletion of the abundant plasma proteins, 2-dimensional gel electrophoresis (2-DE), image analysis, and mass spectrometry in the plasma between normal healthy donors and vivax malaria patients. The results showed that the expression level of haptoglobin had become significantly lower or undetectable in the plasma of vivax malaria patients due to proteolytic cleavage when compared to healthy donors on 2-DE gels. Meanwhile, serum amyloid A protein was significantly increased in vivax malaria patient's plasma with high statistical values. These 2 proteins are common acute phase reactants and further large scale evaluation with a larger number of patient's will be necessary to establish the possible clinical meaning of the existential changes of these proteins in vivax malaria patients. However, our proteomic analysis suggests the feasible values of some plasma proteins, such as haptoglobin and serum amyloid A, as associating factor candidates for vivax malaria.
Bahk
Young Yil
YY
Department of Integrated OMICS for Biomedical Sciences, Graduate School, Yonsei University, Seoul 120-749, Korea.
Na
Byoung-Kuk
BK
Cho
Shin-Hyeong
SH
Kim
Jung-Yeon
JY
Lim
Kook-Jin
KJ
Kim
Tong-Soo
TS
D001798:Blood Proteins / Q000032:analysis; D015180:Electrophoresis, Gel, Two-Dimensional; D006242:Haptoglobins / Q000032:analysis / Q000378:metabolism*; D006801:Humans; D016780:Malaria, Vivax / Q000175:diagnosis / Q000378:metabolism* / Q000469:parasitology; D010966:Plasmodium vivax / Q000502:physiology; D040901:Proteomics / Q000379:methods*; D000685:Serum Amyloid A Protein / Q000032:analysis / Q000378:metabolism*
D001798:Blood Proteins; D006242:Haptoglobins; D000685:Serum Amyloid A Protein