Expression of PAT and NPT II proteins during the developmental stages of a genetically modified pepper developed in Korea.
Estimation of the protein levels introduced in a biotechnology-derived product is conducted as part of an overall safety assessment. An enzyme-linked immunosorbent assay (ELISA) was used to analyze phosphinothricin acetyltransferase (PAT) and neomycin phosphotransferase II (NPT II) protein expression in a genetically modified (GM) pepper plant developed in Korea. PAT and NPT II expression levels, based on both dry weight and fresh weight, were variable among different plant generations and plant sections from isolated genetically modified organism (GMO) fields at four developmental stages. PAT expression was highest in leaves at anthesis (11.44 μg/gdw and 2.17 μg/gfw) and lowest in roots (0.12 μg/gdw and 0.01 μg/gfw). NPT II expression was also highest in leaves at anthesis (17.31 μg/gdw and 3.41 μg/gfw) and lowest in red pepper (0.65 μg/gdw and 0.12 μg/gfw). In pollen, PAT expression was 0.59-0.62 μg/gdw, while NPT II was not detected. Both PAT and NPT II showed a general pattern of decreased expression with progression of the growing season. As expected, PAT and NPT II protein expression was not detectable in control pepper plants.
Kim
Hyo Jin
HJ
National Academy of Agricultural Science, Rural Development Administration, Suwon, Gyeonggi-do, 441-707, Korea.
Lee
Si Myung
SM
Kim
Jae Kwang
JK
Ryu
Tae Hun
TH
Suh
Seok Cheol
SC
Cho
Hyun Suk
HS
D000123:Acetyltransferases / Q000235:genetics* / Q000378:metabolism; D002212:Capsicum / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development*; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D019868:Kanamycin Kinase / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development*; D056910:Republic of Korea
D000123:Acetyltransferases; C056243:phosphinothricin N-acetyltransferase; D019868:Kanamycin Kinase