title: Protective action of resveratrol in human skin: possible involvement of specific receptor binding sites.
abstract: BACKGROUND
  Resveratrol is a plant-derived polyphenol with purported protecting action on various disorders associated with aging. It has been suggested that resveratrol could exert its protective action by acting on specific plasma membrane polyphenol binding sites (Han Y.S., et al. (2006) J Pharmacol Exp Ther 318:238-245). The purpose of this study was to investigate, in human skin, the possible existence of specific binding sites that mediate the protective action of resveratrol.
  
  
  RESULTS
  Using human skin tissue, we report here the presence of specific [(3)H]-resveratrol binding sites (K(D)  =  180 nM) that are mainly located in the epidermis. Exposure of HaCaT cells to the nitric oxide free radical donor sodium nitroprusside (SNP; 0.3-3 mM) resulted in cell death which was reduced by resveratrol (EC(50)  =  14.7 µM), and to a much lesser extent by the resveratrol analogue piceatannol (EC(50)  =  95 µM) and epigallocatechin gallate (EC(50)  =  200 µM), a green-tea derived polyphenol. The protective action of resveratrol likely relates to its anti-apoptotic effect since at the same range of concentration it was able to reduce both the number of apoptotic cells as well as mitochondrial apoptotic events triggered by SNP.
  
  
  CONCLUSIONS
  Taken together, these findings suggest that resveratrol, by acting on specific polyphenol binding sites in epidermis, may be useful to prevent skin disorders associated with aging.
authors.affiliation: Department of Psychiatry, Douglas Mental Health University Institute, McGill University, Montréal, Quebec, Canada.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Bastianetto
authors.forename: Yvan
authors.initials: Y
authors.lastname: Dumont
authors.forename: Albert
authors.initials: A
authors.lastname: Duranton
authors.forename: Freya
authors.initials: F
authors.lastname: Vercauteren
authors.forename: Lionel
authors.initials: L
authors.lastname: Breton
authors.forename: Rémi
authors.initials: R
authors.lastname: Quirion
mesh_terms: D000368:Aged; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002478:Cells, Cultured; D005260:Female; D005419:Flavonoids / Q000378:metabolism; D006801:Humans; D008875:Middle Aged; D020030:Nitric Oxide Donors / Q000494:pharmacology; D010636:Phenols / Q000378:metabolism; D059808:Polyphenols; D020011:Protective Agents / Q000494:pharmacology*; D011485:Protein Binding / Q000187:drug effects; D011956:Receptors, Cell Surface / Q000378:metabolism; D000077185:Resveratrol; D012867:Skin / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D013267:Stilbenes / Q000494:pharmacology*
chemical_list: D005419:Flavonoids; D020030:Nitric Oxide Donors; D010636:Phenols; D059808:Polyphenols; D020011:Protective Agents; D011956:Receptors, Cell Surface; D013267:Stilbenes; D000077185:Resveratrol