title: High-resolution ¹H magic angle spinning NMR spectroscopy of intact Arctic char (Salvelinus Alpinus) muscle. Quantitative analysis of n-3 fatty acids, EPA and DHA.
abstract: The lipid and small metabolite profiles from intact muscles of Arctic char were investigated using (1)H high-resolution magic angle spinning ((1)H HR-MAS) NMR spectroscopy. Not only the total n-3 fatty acid content but also the eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) contents of the muscle were obtained from the (1)H HR-MAS NMR spectra without pretreatment of the tissue or lipophilic extraction. A number of small metabolites could also be observed, where creatine/phosphocreatine, anserine and taurine were the most abundant. Thus, the use of (1)H HR-MAS NMR led to simplified analysis techniques that can give direct information on the nutritional value of the fish.
authors.affiliation: Department of Chemistry, Swedish University of Agricultural Sciences, P.O. Box 7015, SE-750 07, Uppsala, Sweden.
authors.forename: Gustav
authors.initials: G
authors.lastname: Nestor
authors.forename: Johan
authors.initials: J
authors.lastname: Bankefors
authors.forename: Christian
authors.initials: C
authors.lastname: Schlechtriem
authors.forename: Eva
authors.initials: E
authors.lastname: Brännäs
authors.forename: Jana
authors.initials: J
authors.lastname: Pickova
authors.forename: Corine
authors.initials: C
authors.lastname: Sandström
mesh_terms: D000818:Animals; D004281:Docosahexaenoic Acids / Q000032:analysis* / Q000378:metabolism; D015118:Eicosapentaenoic Acid / Q000032:analysis* / Q000378:metabolism; D015525:Fatty Acids, Omega-3 / Q000032:analysis* / Q000378:metabolism; D005396:Fish Products / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy / Q000295:instrumentation / Q000379:methods*; D018482:Muscle, Skeletal / Q000737:chemistry* / Q000378:metabolism; D014337:Trout / Q000378:metabolism*
chemical_list: D015525:Fatty Acids, Omega-3; D004281:Docosahexaenoic Acids; D015118:Eicosapentaenoic Acid