title: Cryptotomography: reconstructing 3D Fourier intensities from randomly oriented single-shot diffraction patterns.
abstract: We reconstructed the 3D Fourier intensity distribution of monodisperse prolate nanoparticles using single-shot 2D coherent diffraction patterns collected at DESY's FLASH facility when a bright, coherent, ultrafast x-ray pulse intercepted individual particles of random, unmeasured orientations. This first experimental demonstration of cryptotomography extended the expansion-maximization-compression framework to accommodate unmeasured fluctuations in photon fluence and loss of data due to saturation or background scatter. This work is an important step towards realizing single-shot diffraction imaging of single biomolecules.
authors.affiliation: Laboratory of Atomic and Solid State Physics, Cornell High Energy Synchrotron Source (CHESS), Cornell University, Ithaca, New York 14853-2501, USA.
authors.forename: N D
authors.initials: ND
authors.lastname: Loh
authors.forename: M J
authors.initials: MJ
authors.lastname: Bogan
authors.forename: V
authors.initials: V
authors.lastname: Elser
authors.forename: A
authors.initials: A
authors.lastname: Barty
authors.forename: S
authors.initials: S
authors.lastname: Boutet
authors.forename: S
authors.initials: S
authors.lastname: Bajt
authors.forename: J
authors.initials: J
authors.lastname: Hajdu
authors.forename: T
authors.initials: T
authors.lastname: Ekeberg
authors.forename: F R N C
authors.initials: FR
authors.lastname: Maia
authors.forename: J
authors.initials: J
authors.lastname: Schulz
authors.forename: M M
authors.initials: MM
authors.lastname: Seibert
authors.forename: B
authors.initials: B
authors.lastname: Iwan
authors.forename: N
authors.initials: N
authors.lastname: Timneanu
authors.forename: S
authors.initials: S
authors.lastname: Marchesini
authors.forename: I
authors.initials: I
authors.lastname: Schlichting
authors.forename: R L
authors.initials: RL
authors.lastname: Shoeman
authors.forename: L
authors.initials: L
authors.lastname: Lomb
authors.forename: M
authors.initials: M
authors.lastname: Frank
authors.forename: M
authors.initials: M
authors.lastname: Liang
authors.forename: H N
authors.initials: HN
authors.lastname: Chapman
mesh_terms: D005240:Feasibility Studies; D005290:Ferric Compounds / Q000737:chemistry; D005583:Fourier Analysis; D021621:Imaging, Three-Dimensional / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry; D012542:Scattering, Radiation; D014054:Tomography / Q000379:methods*
chemical_list: D005290:Ferric Compounds; C000499:ferric oxide