Integrated set-up of sampling, sample treatment and assay of an intracellular protein during fermentation.
A complete system for on-line sampling, sample handling and analysis of fermentation processes has been set up. The system is designed such that a sample is taken from the fermentor, the metabolism of microbial cells is instantaneously arrested, the cells are lysed in order to release intracellular compounds and in a subsequent binding assay the content of target molecules is monitored. The system is constructed based on the flow-injection analysis concept. Good agreement between on-line analysis and off-line analysis is reported.
Mazlomi
Mohammadali
M
Biotechnology Department, Lund University, Sweden.
Hedström
Martin
M
Mattiasson
Bo
B
D019149:Bioreactors; D001709:Biotechnology / Q000379:methods*; D004926:Escherichia coli / Q000254:growth & development / Q000378:metabolism; D005285:Fermentation; D017022:Flow Injection Analysis / Q000379:methods*; D042541:Intracellular Space; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000378:metabolism; D013696:Temperature; D001617:beta-Glucosidase / Q000096:biosynthesis / Q000378:metabolism
D011993:Recombinant Fusion Proteins; D001617:beta-Glucosidase