title: Determination of platinum originated from antitumoral drugs in human urine by atomic absorption spectrometric methods.
abstract: Cisplatin and carboplatin are the most common platinum-based drugs used in cancer treatment. Pharmacokinetic investigations, the evaluation of the body burden during the treatment, as well as baseline levels of platinum in humans have attracted great interest. Thus, accurate analytical methods for fast and easy Pt monitoring in clinical samples become necessary. In the present study atomic absorption spectrometric methods for the determination of platinum in the forms of cisplatin and carboplatin in human urine were investigated. Platinum, in these different forms, could be determined in urine, after simple sample dilution. Regarding electrothermal atomic absorption spectrometry, the optimum parameters were defined by a central composite design optimization. Multiplicative matrix effects were overcome by using a mixture of HCl and NaCl as modifier. The limit of detection (LOD) was 0.004 mgL(-1) of platinum in the original sample. For the analysis of more concentrated samples, high resolution continuous source flame atomic absorption spectrometry was also investigated. Flame conditions were optimized by a multivariate D-optimal design, using as response the sum of the analyte addition calibration slopes and their standard deviations. Matrix matched external calibration with PtCl(2) calibration solutions, was possible, and the LOD was 0.06 mgL(-1) in the original sample. The results obtained by the proposed procedures were also in good agreement with those obtained by an independent comparative procedure.
authors.affiliation: Pontifícia Universidade Católica do Rio de Janeiro (PUC-Rio), Departamento de Química, Rio de Janeiro, RJ, Brazil.
authors.forename: Anilton Coelho
authors.initials: AC
authors.lastname: da Costa
authors.forename: Mariana Antunes
authors.initials: MA
authors.lastname: Vieira
authors.forename: Aderval Severino
authors.initials: AS
authors.lastname: Luna
authors.forename: Reinaldo Calixto
authors.initials: RC
authors.lastname: de Campos
mesh_terms: D000970:Antineoplastic Agents / Q000493:pharmacokinetics / Q000652:urine*; D002138:Calibration; D016190:Carboplatin / Q000493:pharmacokinetics / Q000652:urine*; D002945:Cisplatin / Q000493:pharmacokinetics / Q000652:urine*; D006801:Humans; D066298:In Vitro Techniques; D057230:Limit of Detection; D017671:Platinum Compounds / Q000737:chemistry; D015203:Reproducibility of Results; D013054:Spectrophotometry, Atomic / Q000295:instrumentation / Q000379:methods*
chemical_list: D000970:Antineoplastic Agents; D017671:Platinum Compounds; C030173:platinum chloride; D016190:Carboplatin; D002945:Cisplatin