title: A fluorescence-based Coomassie Blue protocol for two-dimensional gel-based proteomics.
abstract: A sensitive and convenient "visible SYPRO" staining protocol was developed for visualizing proteins after SDS-PAGE. Gels were sensitized with SYPRO Ruby and then stained with the Coomassie Brilliant Blue G-250 protocol (Blue Silver). This combined protocol had similar or better linearity than staining with only SYPRO Ruby or Blue Silver, respectively. In addition, this method was more sensitive than that of Blue Silver, simpler than that of SYPRO Ruby, and compatible with subsequent mass spectrometry analysis.
authors.affiliation: State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, No. 2, Yuan Ming Yuan West Road, Haidian District, Beijing, 100193, China.
authors.forename: Yongxin
authors.initials: Y
authors.lastname: Yang
authors.forename: Jiaqi
authors.initials: J
authors.lastname: Wang
authors.forename: Dengpan
authors.initials: D
authors.lastname: Bu
authors.forename: Leying
authors.initials: L
authors.lastname: Zhang
authors.forename: Shanshan
authors.initials: S
authors.lastname: Li
authors.forename: Lingyun
authors.initials: L
authors.lastname: Zhou
authors.forename: Hongyang
authors.initials: H
authors.lastname: Wei
mesh_terms: D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005453:Fluorescence; D040901:Proteomics / Q000379:methods*; D012394:Rosaniline Dyes / Q000378:metabolism*; D012680:Sensitivity and Specificity; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D012394:Rosaniline Dyes; C048139:Coomassie blue