Method for the decellularization of intact rat liver.
We have developed a method for the decellularization of whole rat livers by perfusion with increasing concentrations of detergents. This procedure resulted in an intact, decellularized organ with an intact liver capsule. These decellularized organs were analyzed by immunohistochemistry, and retained an appropriate distribution of extracellular matrix components. The laminin basement membranes of the liver vasculature also remain intact. These acellular vessel remnants were strong enough to be cannulated, providing a convenient means for the delivery of cells to areas deep within the decellularized organ. Cannulation of the extrahepatic vessel remnants allow for media to be circulated through the decellularized organ. These decellularized livers provide a natural matrix for research in the fields of bio-artificial livers and liver engineering.
Shupe
Thomas
T
Department of Pathology, Immunology and Laboratory Medicine, College of Medicine, University of Florida, Gainesville, FL, USA. shupe@pathology.ufl.edu
Williams
Matthew
M
Brown
Alicia
A
Willenberg
Bradley
B
Petersen
Bryon E
BE
D000818:Animals; D008099:Liver / Q000098:blood supply / Q000166:cytology*; D010477:Perfusion; D051381:Rats; D011916:Rats, Inbred F344; D046509:Tissue Culture Techniques / Q000379:methods*