The paradox of multiplex DNA melting on a surface.
Under equilibrium conditions, there are two regimes of target capture on a surface--target limited and probe limited. In the probe limited regime, the melting curve from multiplex target dissociation from the surface exhibits a single transition due to a reverse displacement mechanism of the low affinity species. The melting curve cannot be used in analytical methods to resolve heteroduplexes; only with the simplex system can proper thermodynamics be obtained.
Williams
Layne
L
Department of Electrical and Computer Engineering, University of Utah, USA.
Blair
Steve
S
Chagovetz
Alexander
A
Fish
Daniel J
DJ
Benight
Albert S
AS
D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D009691:Nucleic Acid Denaturation; D013816:Thermodynamics; D044366:Transition Temperature
D004247:DNA