Coated cross-species antibodies by mannosamine-biotin adduct confer protection against snake venom without eliciting humoral immune response.
Passive immunization with cross-species antibodies triggers the patient's immune response, thereby preventing repeated treatment. Mannosamine-biotin adduct (MBA) has been described as a masking agent for immunogenic reduction and here, the immunogenicity and biological activity of MBA-coated horse anti-viper venom (hsIgG) were compared to those of uncoated or PEGylated hsIgG. In in vitro tests, hsIgG binding was not affected by MBA conjugation. The immune response to hsIgG-MBA was about 8-fold and 32-fold lower than to PEG-coated and uncoated hsIgG, respectively. In vivo, hsIgG-MBA showed efficient venom-neutralization activity. We thus demonstrate the feasibility of using MBA as a masking agent for passive immunization with cross-species antibodies.
Gefen
Tal
T
MIGAL - Galilee Technology Center, P.O. Box 831, Kiryat Shmona 11016, Israel.
Pitcovski
Jacob
J
Vaya
Jacob
J
Khatib
Soliman
S
Krispel
Simi
S
Heller
E Dan
ED
Gaberman
Elena
E
Gorodetsky
Raphael
R
Aizenshtein
Elina
E
D000818:Animals; D000997:Antivenins / Q000737:chemistry* / Q000276:immunology*; D001710:Biotin / Q000378:metabolism*; D003429:Cross Reactions; D006595:Hexosamines / Q000378:metabolism*; D006736:Horses; D006801:Humans; D007116:Immunization, Passive / Q000379:methods*; D007074:Immunoglobulin G / Q000737:chemistry / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009500:Neutralization Tests; D011485:Protein Binding; D012910:Snake Venoms / Q000037:antagonists & inhibitors* / Q000276:immunology
D000997:Antivenins; D006595:Hexosamines; D007074:Immunoglobulin G; D012910:Snake Venoms; C008322:mannosamine; D001710:Biotin