Screening of filamentous fungi to identify biocatalysts for lupeol biotransformation.
The goal of the study was to evaluate the ability of filamentous fungi to biotransform the pentacyclic triterpene lupeol. The microbial transformations were carried out in shake flasks in different media. Experiments were also run with control flasks. Samples of each culture were taken every 24 hours, extracted with ethyl acetate, and analyzed by GC-MS. The biotransformation of lupeol by Aspergillus ochraceus and Mucor rouxii afforded two compounds in each culture, which were detected in the cultures developed for more than seven days only in the Koch's K1 medium. The obtained data demonstrated that A. ochraceus is a good biocatalyst to introduce double bonds in the lupeol structure, whereas M. rouxii exhibits ability to biocatalyze oxygen insertions in that pentacyclic triterpene. Mass spectrometry was demonstrated to be an efficient analytical method to select promising biocatalysts for the compound investigated in this study. The biotransformation processes were influenced by the culture medium and incubation period. The obtained results open the perspective of using A. ochraceus and M. rouxii in pentacyclic triterpene biotransformations.
de Carvalho
Tatiane C
TC
Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil. carvalho_tc@ yahoo.com.br
Polizeli
Aline M
AM
Turatti
Izabel C C
IC
Severiano
Marcela E
ME
de Carvalho
Carlos E
CE
Ambrósio
Sérgio R
SR
Crotti
Antônio E M
AE
de Figueiredo
Uir S
US
Vieira
Paulo C
PC
Furtado
Niege A J C
NA
D000893:Anti-Inflammatory Agents / Q000378:metabolism*; D001235:Aspergillus ochraceus / Q000378:metabolism; D001711:Biotransformation; D002384:Catalysis; D005658:Fungi / Q000737:chemistry / Q000378:metabolism*; D013058:Mass Spectrometry / Q000379:methods; D009089:Mucor / Q000378:metabolism; D053978:Pentacyclic Triterpenes / Q000378:metabolism*; D014315:Triterpenes / Q000378:metabolism; D000094042:Lupanes
D000893:Anti-Inflammatory Agents; D053978:Pentacyclic Triterpenes; D014315:Triterpenes; C010480:lupeol; D000094042:Lupanes