title: Molecular characterization, structural analysis and determination of host range of a novel bacteriophage LSB-1.
abstract: BACKGROUND
  Bacteriophages (phages) are widespread in the environment and play a crucial role in the evolution of their bacterial hosts and the emergence of new pathogens.
  
  
  RESULTS
  LSB-1, a reference coliphage strain, was classified as a member of the Podoviridae family with a cystic form (50 ± 5 nm diameter) and short tail (60 ± 5 nm long). The double stranded DNA was about 30 kilobase pairs in length. We identified its host range and determined the gp17 sequences and protein structure using shotgun analysis and bioinformatics technology.
  
  
  CONCLUSIONS
  Coliphage LSB-1 possesses a tailspike protein with endosialidase activity which is probably responsible for its specific enteroinvasive E.coli host range within the laboratory.
authors.affiliation: Department of Epidemiology, Faculty of Preventive Medicine, Third Military Medical University, Chongqing 400038, China.
authors.forename: Yaming
authors.initials: Y
authors.lastname: Chai
authors.forename: Hongyan
authors.initials: H
authors.lastname: Xiong
authors.forename: Xiangyu
authors.initials: X
authors.lastname: Ma
authors.forename: Liqing
authors.initials: L
authors.lastname: Cheng
authors.forename: Guorong
authors.initials: G
authors.lastname: Huang
authors.forename: Zhonglin Rao Lin
authors.initials: ZR
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D003090:Coliphages / Q000145:classification / Q000235:genetics* / Q000502:physiology* / Q000648:ultrastructure; D019295:Computational Biology; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D004926:Escherichia coli / Q000821:virology; D058507:Host Specificity; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010802:Phylogeny; D017902:Podoviridae / Q000145:classification / Q000235:genetics* / Q000502:physiology* / Q000648:ultrastructure; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000235:genetics; D014771:Virion / Q000648:ultrastructure
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins