title: Renal expression of organic anion transporter Oat5 in rats and mice exhibits the female-dominant sex differences.
abstract: The organic anion transporter 5 (Oat5, Slc22a19) was previously localized to the brush-border of proximal tubule (PT) S3 segment in rat and mouse kidneys. Here we report on sex hormone-regulated expression of Oat5 in rat kidneys, after reinvestigating: a) expression of its mRNA by end-point and real time RT-PCR in the tissue, b) abundance of its protein by Western blotting (WB) in isolated membranes, and c) immunolocalization in tissue cryosections. In untreated male (M) and female (F) adult rats, the expression of Oat5 mRNA was predominant in the outer stripe (OS), exhibiting sex differences (M<F), upregulated by castration, and unaffected by ovariectomy. In castrated M, testosterone treatment strongly downregulated, whereas estradiol and progesterone treatment weakly upregulated its expression. By WB, a single protein band of ~72 kDa in variously-treated animals exhibited a density pattern comparable to that of mRNA. By immunostaining, Oat5 protein was localized to the brush-border of S1/S2 in the cortex (CO) (weakly) and in S3 of the OS and medullary rays (strongly) with the F-dominant intensity. In variously-treated rats, the immunostaining pattern matched that of mRNA and WB data. In prepubertal rats, the renal expression of Oat5 mRNA and protein was weak and sex-independent. In adult mice, the sex-dependent pattern of renal Oat5 protein expression was comparable to that in rats. Therefore, the renal expression of Oat5 in rats (and mice) exhibits zonal (CO<OS) and sex differences (M<F), which appear after puberty, largely due to androgen-driven downregulation of its mRNA and protein expression.
authors.affiliation: Molecular Toxicology, Institute for Medical Research and Occupational Health, Zagreb, Croatia. sabolic@imi.hr
authors.forename: Davorka
authors.initials: D
authors.lastname: Breljak
authors.forename: Marija
authors.initials: M
authors.lastname: Ljubojevic
authors.forename: Daniela
authors.initials: D
authors.lastname: Balen
authors.forename: Vilim
authors.initials: V
authors.lastname: Zlender
authors.forename: Hrvoje
authors.initials: H
authors.lastname: Brzica
authors.forename: Vedran
authors.initials: V
authors.lastname: Micek
authors.forename: Marija
authors.initials: M
authors.lastname: Kusan
authors.forename: Naohiko
authors.initials: N
authors.lastname: Anzai
authors.forename: Ivan
authors.initials: I
authors.lastname: Sabolic
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002369:Castration; D029382:Dicarboxylic Acid Transporters / Q000096:biosynthesis*; D005260:Female; D007150:Immunohistochemistry; D007668:Kidney / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D027361:Organic Anion Transporters / Q000096:biosynthesis*; D010052:Ovariectomy; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012727:Sex Characteristics
chemical_list: D029382:Dicarboxylic Acid Transporters; D027361:Organic Anion Transporters; D012333:RNA, Messenger; C488753:Slc22a19 protein, mouse; C506835:Slc22a24 protein, rat