Zirconia layer coated mesoporous silica microspheres used for highly specific phosphopeptide enrichment.
Zirconia layer coated mesoporous silica microspheres with mesostructured cellular foams (MCFs) were prepared by NH(3)/water vapor-induced internal hydrolysis method. Zirconia layer coated MCF microspheres were characterized by SEM, XRD, N(2) sorption, UV, and chromatographic analysis, and explored for enrichment of phosphopeptides. ZrO(2)/MCF microspheres in solid-phase extraction (SPE) mode demonstrated much higher selectivity and higher efficiency towards phosphopeptide enrichment than bulk ZrO(2) particles. In particular, the selectivities of ZrO(2)/MCF microspheres towards multi-phosphopeptides are even higher than that of the widely used commercial TiO(2) microparticles. The ZrO(2)/MCF microspheres were also applied to enrich endogenous phosphopeptides from human serum, and twelve endogenous phosphorylated peptides could be specifically enriched.
Wan
Huihui
H
Key Lab of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian, China.
Yan
Jingyu
J
Yu
Long
L
Zhang
Xiuli
X
Xue
Xingya
X
Li
Xiuling
X
Liang
Xinmiao
X
D006801:Humans; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D010748:Phosphopeptides / Q000097:blood / Q000302:isolation & purification*; D010766:Phosphorylation; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry*; D052616:Solid Phase Extraction; D021241:Spectrometry, Mass, Electrospray Ionization; D013499:Surface Properties; D014961:X-Ray Diffraction; D015040:Zirconium / Q000737:chemistry*
D010748:Phosphopeptides; D012822:Silicon Dioxide; D015040:Zirconium; C028541:zirconium oxide