Food analysis and food authentication by peptide nucleic acid (PNA)-based technologies.
This tutorial review will address the issue of DNA determination in food by using Peptide Nucleic Acid (PNA) probes with different technological platforms, with a particular emphasis on the applications devoted to food authentication. After an introduction aimed at describing PNAs structure, binding properties and their use as genetic probes, the review will then focus specifically on the use of PNAs in the field of food analysis. In particular, the following issues will be considered: detection of genetically modified organisms (GMOs), of hidden allergens, of microbial pathogens and determination of ingredient authenticity. Finally, the future perspectives for the use of PNAs in food analysis will be briefly discussed according to the most recent developments.
Sforza
Stefano
S
Department of Organic and Industrial Chemistry, University of Parma, Parco Area delle Scienze 17a, University Campus, I-43124, Parma, Italy. stefano.sforza@unipr.it
Corradini
Roberto
R
Tedeschi
Tullia
T
Marchelli
Rosangela
R
D000485:Allergens / Q000737:chemistry / Q000276:immunology; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D005504:Food Analysis; D005506:Food Contamination / Q000032:analysis; D053768:Metal Nanoparticles / Q000737:chemistry; D030781:Organisms, Genetically Modified / Q000235:genetics / Q000382:microbiology; D020135:Peptide Nucleic Acids / Q000737:chemistry*
D000485:Allergens; D020135:Peptide Nucleic Acids; D004247:DNA