Immobilization of biomolecules onto surfaces according to ultraviolet light diffraction patterns.
We developed a method for immobilization of biomolecules onto thiol functionalized surfaces according to UV diffraction patterns. UV light-assisted molecular immobilization proceeds through the formation of free, reactive thiol groups that can bind covalently to thiol reactive surfaces. We demonstrate that, by shaping the pattern of the UV light used to induce molecular immobilization, one can control the pattern of immobilized molecules onto the surface. Using a single-aperture spatial mask, combined with the Fourier transforming property of a focusing lens, we show that submicrometer (0.7 μm) resolved patterns of immobilized prostate-specific antigen biomolecules can be created. If a dual-aperture spatial mask is used, the results differ from the expected Fourier transform pattern of the mask. It appears as a superposition of two diffraction patterns produced by the two apertures, with a fine structured interference pattern superimposed.
Petersen
Steffen Bjørn
SB
Nanobiotechnology Group, Department of Physics and Nanotechnology, Aalborg University, Skjernvej 4A, Aalborg, Denmark. sp@nano.aau.dk
Kold di Gennaro
Ane
A
Neves-Petersen
Maria Teresa
MT
Skovsen
Esben
E
Parracino
Antonietta
A
D005583:Fourier Analysis; D055767:Immobilized Proteins; D007909:Lenses; D055668:Photochemical Processes; D017430:Prostate-Specific Antigen / Q000528:radiation effects*; D013438:Sulfhydryl Compounds / Q000494:pharmacology; D013499:Surface Properties / Q000187:drug effects; D014466:Ultraviolet Rays
D055767:Immobilized Proteins; D013438:Sulfhydryl Compounds; D017430:Prostate-Specific Antigen