Modern determination of complement activation.
Modern, that is, accurate determination of complement activation in vivo requires the simultaneous determination of both naive and activated complement proteins. This is best achieved by the use of native-restricted and neoepitope-specific monoclonal antibodies in sensitive enzyme-linked immunosorbent assays. Several confounding factors also have to be taken into account to allow a correct interpretation of the results.
Oppermann
Martin
M
Abteilung Zelluläre und Molekulare Immunologie, Zentrum für Hygiene und Humangenetik, Humboldtallee 34, Göttingen, Germany. mopperm@gwdg.de
Würzner
Reinhard
R
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D003167:Complement Activation / Q000276:immunology*; D015941:Complement Hemolytic Activity Assay / Q000379:methods; D003171:Complement Pathway, Classical / Q000276:immunology*; D003165:Complement System Proteins / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D011485:Protein Binding; D011951:Receptors, Complement / Q000378:metabolism
D000911:Antibodies, Monoclonal; D011951:Receptors, Complement; D003165:Complement System Proteins