title: Shinella daejeonensis sp. nov., a nitrate-reducing bacterium isolated from sludge of a leachate treatment plant.
abstract: A Gram-negative-staining, motile, rod-shaped, aerobic bacterial strain, designated MJ02(T), was isolated from sludge of a leachate treatment plant in Daejeon (South Korea) and was characterized to determine its taxonomic position by using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that strain MJ02(T) belonged to the family Rhizobiaceae, class Alphaproteobacteria, and was most closely related to Shinella yambaruensis MS4(T) (97.6 % sequence similarity) and Shinella fusca DC-196(T) (97.5 %). The G+C content of the genomic DNA of strain MJ02(T) was 64.3 mol%. The detection of a quinone system with ubiquinone Q-10 as the predominant respiratory lipoquinone and a fatty acid profile with C₁₈:₁ω7c (45.8 %) and C₁₆:₀ (21.8 %) as the major components supported the affiliation of strain MJ02(T) to the genus Shinella. However, strain MJ02(T) exhibited relatively low levels of DNA-DNA relatedness with respect to S. fusca DSM 21319(T) (17±7 %) and S. yambaruensis KACC 14483(T) (12±6 %), showing clearly that the isolate constituted a new genospecies. Strain MJ02(T) could be clearly differentiated from its phylogenetic neighbours on the basis of several phenotypic, genotypic and chemotaxonomic features. Therefore, strain MJ02(T) is considered to represent a novel species of the genus Shinella, for which the name Shinella daejeonensis sp. nov. is proposed. The type strain is MJ02(T) ( = KCTC 22450(T) = JCM 16236(T)).
authors.affiliation: Research and Development Division, H-Plus Eco Ltd, BVC 301, KRIBB, Eoeun-dong, Yuseong-gu, Daejeon 305-333, Republic of Korea.
authors.forename: Myungjin
authors.initials: M
authors.lastname: Lee
authors.affiliation: School of Civil and Environmental Engineering, Yonsei University, Seoul 120-749, Republic of Korea.|Research and Development Division, H-Plus Eco Ltd, BVC 301, KRIBB, Eoeun-dong, Yuseong-gu, Daejeon 305-333, Republic of Korea.
authors.forename: Sung-Geun
authors.initials: SG
authors.lastname: Woo
authors.affiliation: Research and Development Division, H-Plus Eco Ltd, BVC 301, KRIBB, Eoeun-dong, Yuseong-gu, Daejeon 305-333, Republic of Korea.
authors.forename: Leonid N
authors.initials: LN
authors.lastname: Ten
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D007723:Korea; D008124:Locomotion; D008969:Molecular Sequence Data; D009566:Nitrates / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D010084:Oxidation-Reduction; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012230:Rhizobiaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D009566:Nitrates; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D012722:Sewage