Prevention of murine acute graft-versus-host disease by recipient-derived paired immunoglobulin-like receptor B lentivirus-transfected dendritic cells.
Direct interaction between T-suppressor and dendritic cells (DCs) results in DC tolerance by inducing upregulation of immunoglobulin-like transcript (ILT) 3 and ILT4. DCs were treated with a lentiviral vector containing paired immunoglobulin-like B gene (PIR-B) DCs and the effect of PIR-B-DCs on graft-versus-host disease (GVHD) was analyzed after allogeneic bone marrow (BM) transplantation (BMT). Therefore, 1 × 10⁶ recipient-derived PIR-B-DCs were injected into BALB/c (H-2k(d)) mice using BM-splenocyte grafts from major histocompatibility complex-disparate C57BL/6 (H-2k(b)). Our results showed that PIR-B-DCs deficient in surface costimulatory molecules had higher PIR-B protein expression than immature DCs and interleukin 10-treated DCs. Survival analysis showed PIR-B-DC cotransplantation resulted in significant prolongation of allograft survival (mean survival time: 46.0 ± 13.6 vs. 17.4 ± 3.6 days in untreated MST; p < 0.01). Furthermore, samples from the liver and skin of a mouse did not show clinical or histological signs of GVHD following the injection of PIR-B-DCs. These results demonstrated that PIR-B-DC cotransplantation may attenuate the severity of GVHD after BMT.
Zhao
Juan
J
Institute of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People's Republic of China.
Luo
Yi
Y
Wang
Xiaochong
X
Zhou
Hao
H
Li
Qiubai
Q
You
Yong
Y
Zou
Ping
P
D000818:Animals; D001483:Base Sequence; D016026:Bone Marrow Transplantation / Q000009:adverse effects / Q000276:immunology; D017931:DNA Primers / Q000235:genetics; D003713:Dendritic Cells / Q000276:immunology / Q000637:transplantation; D015870:Gene Expression; D005802:Genes, MHC Class II; D005822:Genetic Vectors; D006086:Graft vs Host Disease / Q000235:genetics / Q000276:immunology / Q000473:pathology / Q000517:prevention & control*; D016086:Lentivirus / Q000235:genetics; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D012333:RNA, Messenger / Q000235:genetics; D011971:Receptors, Immunologic / Q000235:genetics* / Q000276:immunology*; D014162:Transfection; D014184:Transplantation, Homologous
D017931:DNA Primers; C105949:Pirb protein, mouse; D012333:RNA, Messenger; D011971:Receptors, Immunologic