title: Detection and confirmation of Clostridium botulinum in water used for cooling at a plant producing low-acid canned foods.
abstract: Our laboratory tested water samples used for cooling low-acid canned foods at a canning facility under investigation by the U.S. Food and Drug Administration. We used an enzyme-linked immunosorbent assay with digoxigenin-labeled antibodies (DIG-ELISA) and real-time PCR as screening methods and confirmed the presence of neurotoxin-producing Clostridium botulinum in the samples by mouse bioassay.
authors.affiliation: FDA, Center for Food Safety and Applied Nutrition, College Park, MD 20740, USA.
authors.forename: Amita
authors.initials: A
authors.lastname: Sachdeva
authors.forename: Stephanie L H
authors.initials: SL
authors.lastname: Defibaugh-Chávez
authors.forename: James B
authors.initials: JB
authors.lastname: Day
authors.forename: Donald
authors.initials: D
authors.lastname: Zink
authors.forename: Shashi K
authors.initials: SK
authors.lastname: Sharma
mesh_terms: D000818:Animals; D001431:Bacteriological Techniques / Q000379:methods; D001905:Botulinum Toxins / Q000096:biosynthesis; D001906:Botulism / Q000175:diagnosis / Q000473:pathology; D003014:Clostridium botulinum / Q000302:isolation & purification*; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D019649:Food Industry / Q000379:methods*; D057141:Food, Preserved; D008403:Mass Screening / Q000379:methods; D051379:Mice; D016133:Polymerase Chain Reaction / Q000379:methods; D014481:United States; D014871:Water Microbiology
chemical_list: D001905:Botulinum Toxins