An electrochemical supersandwich assay for sensitive and selective DNA detection in complex matrices.
In a traditional sandwich assay, a DNA target hybridizes to a single copy of the signal probe. Here we employ a modified signal probe containing a methylene blue (a redox moiety) label and a "sticky end." When a DNA target hybridizes this signal probe, the sticky end remains free to hybridize another target leading to the creation of a supersandwich structure containing multiple labels. This leads to large signal amplification upon monitoring by voltammetry.
Xia
Fan
F
Center for Polymers and Organic Solids, University of California, Santa Barbara, California 93106, USA.
White
Ryan J
RJ
Zuo
Xiaolei
X
Patterson
Adriana
A
Xiao
Yi
Y
Kang
Di
D
Gong
Xiong
X
Plaxco
Kevin W
KW
Heeger
Alan J
AJ
D004247:DNA / Q000032:analysis*; D004563:Electrochemistry / Q000379:methods*; D057230:Limit of Detection; D009693:Nucleic Acid Hybridization
D004247:DNA