The effects of ammonium perchlorate on thyroid homeostasis and thyroid-specific gene expression in rat.
Perchlorate, a kind of inorganic chemical, is mainly used in defense industry and widely used in other civilian areas. It was well known that perchlorate exerts its thyrotoxicant effect on thyroid homeostasis via competitive inhibition of iodide uptake. However, some details of mechanism by which perchlorate disturb thyroid homeostasis are unknown and remain to be elucidated. The present study aimed to investigate if iodide insufficiency in the thyroid is the main mechanism by which perchlorate exerts its effect on the thyroid gland. We highlighted and measured the gene expression of NIS, Tg, and TPO which involved in thyroid hormone biosynthesis. Thyroid effects of perchlorate were identified by assessing different responses of these genes at the treatments of perchlorate and iodine deficiency. The results indicated that high dose perchlorate (520 mg kg(-1) b.wt.) can induce a significant decrease in body weight and cause hypertrophy of thyroid gland, with a decreased level of FT3, FT4 and a remarkable increased level of TSH. In addition, the significant decreased gene expression of Thyroglobulin (Tg) and thyroperoxidase (TPO) were both observed at the treatment of high dose perchlorate. These results suggested that perchlorate can suppress gene expression of Tg and TPO which directly involved in biosynthesis of thyroid hormones, and may therefore aggravate the perturbation of thyroid homeostasis in addition to competitive inhibition of iodide uptake.
Wu
Fenghong
F
Institute of Industrial Toxicology, Department of Occupational and Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, People's Republic of China.
Zhou
Xuan
X
Zhang
Rao
R
Pan
Meizhu
M
Peng
Kai-Liang
KL
D000818:Animals; D001835:Body Weight / Q000187:drug effects; D015870:Gene Expression / Q000187:drug effects*; D006706:Homeostasis / Q000187:drug effects*; D006984:Hypertrophy; D007453:Iodide Peroxidase / Q000235:genetics / Q000378:metabolism; D007454:Iodides / Q000378:metabolism*; D007455:Iodine / Q000172:deficiency; D008297:Male; D010472:Perchlorates / Q000633:toxicity*; D000644:Quaternary Ammonium Compounds / Q000633:toxicity*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013954:Thyroglobulin / Q000235:genetics / Q000378:metabolism; D013961:Thyroid Gland / Q000187:drug effects* / Q000473:pathology; D013963:Thyroid Hormones / Q000097:blood
D007454:Iodides; D010472:Perchlorates; D000644:Quaternary Ammonium Compounds; D013963:Thyroid Hormones; D013954:Thyroglobulin; D007455:Iodine; D007453:Iodide Peroxidase; C053506:ammonium perchlorate