Electrochemical study of gelatin as a matrix for the immobilization of horse heart cytochrome c.
The aim of this paper is to emphasize the strength of gelatin as a stable matrix for redox enzymes. Cyclic voltammetry has been applied for a detailed electrochemical study of horse heart cytochrome c (HHC) entrapped in a gelatin matrix immobilized on a gold electrode. The influence of the HHC concentration, the mass percentage of the gelatin and the nature of the gelatin on the electrochemical behaviour of HHC have been described in detail. In addition, attenuated total reflection infrared (ATR-IR) spectroscopy was used to prove the immobilization on a qualitative and conformational level. The thickness of the gelatin film was determined using a non-contact optical profiler. These results open up new perspectives in the development of stable, biocompatible matrices for redox enzymes. The latter has its relevance in the field of biosensor development.
De Wael
Karolien
K
Department of Analytical Chemistry, Ghent University, Krijgslaan 281 S12, B-9000 Ghent, Belgium. Karolien.DeWael@UGent.be
De Belder
Stijn
S
Van Vlierberghe
Sandra
S
Van Steenberge
Geert
G
Dubruel
Peter
P
Adriaens
Annemie
A
D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D045304:Cytochromes c / Q000737:chemistry*; D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods*; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005780:Gelatin / Q000737:chemistry*; D006046:Gold / Q000737:chemistry; D006736:Horses; D020100:Hydrogels; D009206:Myocardium / Q000201:enzymology; D013055:Spectrophotometry, Infrared; D013499:Surface Properties
D004800:Enzymes, Immobilized; D020100:Hydrogels; D006046:Gold; D005780:Gelatin; D045304:Cytochromes c