Investigation of MADS domain transcription factor dynamics in the floral meristem.
To study the importance of intercellular transport for MADS domain transcription factor functioning during floral development, we analyzed the dynamic behavior of fluorescently-tagged MADS domain proteins in transgenic plants by Confocal Laser Scanning Microscopy. These analyses, described in a recent paper in The Plant Journal, provided proof for previous suggestions that the Arabidopsis thaliana C-type protein AGAMOUS has a non-cell-autonomous role in floral meristem integrity. Furthermore, it indicated a possible non-cell-autonomous role for the B-type proteins APETALA3 and PISTILLATA, and the E-type protein SEPALLATA3, through lateral intercellular movement in the floral meristem. In this addendum we compare some of the available fluorescent protein-based technologies for the investigation of transcription factor movements and dynamics.
Urbanus
Susan L
SL
Plant Research International, Droevendaalsesteeg, The Netherlands.
Dinh
Q D Peter
QD
Angenent
Gerco C
GC
Immink
Richard G H
RG
D017360:Arabidopsis / Q000378:metabolism* / Q000528:radiation effects; D029681:Arabidopsis Proteins / Q000378:metabolism*; D035264:Flowers / Q000378:metabolism* / Q000528:radiation effects; D036681:Fluorescence Recovery After Photobleaching; D005456:Fluorescent Dyes / Q000378:metabolism; D008027:Light; D026161:MADS Domain Proteins / Q000378:metabolism*; D018519:Meristem / Q000378:metabolism* / Q000528:radiation effects
C091535:AP1 protein, Arabidopsis; D029681:Arabidopsis Proteins; D005456:Fluorescent Dyes; D026161:MADS Domain Proteins