title: Development and validation of a RP-HPLC method for simultaneous estimation of naproxen and ranitidinehydrochloride.
abstract: A simple, specific and accurate reverse phase liquid chromatographic method has been developed for the simultaneous determination of naproxen and ranitidine HCl. Both the drugs are official with British Pharmacopoeia 2007, but do not involve simultaneous determination of naproxen and ranitidine HCl. The separation was carried out using 4.6 × 250 mm Symmetry Shield TM RP 18 with a particle diameter of 5 µm and mobile phase containing 0.1M orthophosphoric acid: methanol (35:65, pH 3.1) in isocratic mode. The flow rate was 1.00 ml/min and effluent was monitored at 240 nm. The retention times (average) of ranitidine HCl and naproxen were 2.36 min and 12.39 min, respectively. The linearity for naproxen and ranitidine HCl was in the range of 5-35 µg/ml and 1.5-12 µg/ml, respectively. The potencies of naproxen and ranitidine HCl were found 99.40 % and 99.48 %, respectively. The proposed method was validated and successfully applied to the estimation of naproxen and ranitidine HCl in newly formulated combined tablet and in plasma.
authors.affiliation: Department of Pharmacy, Stamford University Bangladesh, Dhaka-1217, Bangladesh. shoume_du@yahoo.com
authors.forename: Tasnuva
authors.initials: T
authors.lastname: Haque
authors.forename: Md Mesbahuddin
authors.initials: MM
authors.lastname: Talukder
authors.forename: Susmita
authors.initials: S
authors.lastname: Laila
authors.forename: Kanij
authors.initials: K
authors.lastname: Fatema
mesh_terms: D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000032:analysis* / Q000097:blood; D002021:Buffers; D002138:Calibration; D002626:Chemistry, Pharmaceutical; D002851:Chromatography, High Pressure Liquid; D004338:Drug Combinations; D006635:Histamine H2 Antagonists / Q000032:analysis* / Q000097:blood; D006801:Humans; D009288:Naproxen / Q000032:analysis* / Q000097:blood; D011899:Ranitidine / Q000032:analysis* / Q000097:blood; D012015:Reference Standards; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D013607:Tablets
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D002021:Buffers; D004338:Drug Combinations; D006635:Histamine H2 Antagonists; D013607:Tablets; D009288:Naproxen; D011899:Ranitidine