title: Fabrication, structure and biological properties of organic acid-derived sol-gel bioactive glasses.
abstract: Sol-gel-derived bioactive glasses (BGs) have been developed for bone tissue regeneration. To develop more reliable bone tissue repair systems, it is necessary to control the morphology and surface textures of bioactive glasses. In this study, we prepared bioactive glasses by sol-gel technology using hydrochloride acid, lactic acid, citric acid and acetic acid as hydrolysis catalysts. We studied effects of acids on the morphology and surface textures, apatite-forming bioactivity and cellular response (cellular attachment and proliferation) of BGs. Results showed that the surface morphology, structure, apatite-forming bioactivity and cellular response of BG particles can be controlled by changing acid species. The hydrochloric acid-derived bioactive glass (HBG) and the acetic acid-derived bioactive glass (ABG) present high surface areas and fast apatite-forming rates. Lactic acid- and citric acid-derived bioactive glasses (LBG, CBG) exhibited nanoscale surface morphology, relatively low surface areas and comparable apatite-forming bioactivity. The results of human marrow mesenchymal stem cell (HMSC) culture exhibited that LBG and CBG have an enhanced effect on the cell proliferation, as compared to HBG, ABG and tissue culture plate. This study suggests that sol-gel bioactive glasses with proper surface textures and apatite-forming rate can affect preliminary cellular proliferation.
authors.affiliation: Biomedical Engineering Institute, South China University of Technology, Guangzhou 510640, People's Republic of China.
authors.forename: Bo
authors.initials: B
authors.lastname: Lei
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Chen
authors.forename: Yingjun
authors.initials: Y
authors.lastname: Wang
authors.forename: Naru
authors.initials: N
authors.lastname: Zhao
authors.forename: Chang
authors.initials: C
authors.lastname: Du
authors.forename: Liming
authors.initials: L
authors.lastname: Fang
mesh_terms: D019342:Acetic Acid / Q000737:chemistry*; D001672:Biocompatible Materials / Q000737:chemistry*; D001854:Bone Marrow Cells / Q000166:cytology; D001842:Bone and Bones / Q000502:physiology; D002384:Catalysis; D002448:Cell Adhesion; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D019343:Citric Acid / Q000737:chemistry*; D017886:Durapatite / Q000737:chemistry; D005782:Gels; D005898:Glass / Q000737:chemistry*; D006801:Humans; D006851:Hydrochloric Acid / Q000737:chemistry; D006868:Hydrolysis; D007700:Kinetics; D019344:Lactic Acid / Q000737:chemistry*; D059630:Mesenchymal Stem Cells / Q000502:physiology; D008855:Microscopy, Electron, Scanning; D010316:Particle Size; D016062:Porosity; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D005782:Gels; D019343:Citric Acid; D019344:Lactic Acid; D017886:Durapatite; D019342:Acetic Acid; D006851:Hydrochloric Acid