Evaluation of the procoagulant activity in the plasma of cancer patients using a thrombin generation assay.
BACKGROUND
Circulating microparticles are reported to play a role in cancer hypercoagulability. The procoagulant properties of microparticles derive from the amount of tissue factor and/or phosphatidylserine that they can expose. The aim of our study is to assess the procoagulant activity, including microparticles' activity, in the plasma of newly diagnosed cancer patients with a simple assay, easy to implement in the laboratory.


METHODS
Newly diagnosed cancer patients (n=31) before the start of anticoagulant or chemotherapy were compared to matched controls. We used a thrombin generation assay in four conditions: 1: addition of 1 pM tissue factor and 4 μM procoagulant phospholipids, 2: without any trigger, 3 and 4: addition of tissue factor or procoagulant phospholipids alone respectively.


RESULTS
When we added only phospholipids, so that thrombin generation is dependent upon endogenous tissue factor, the lag time was significantly shorter in cancer patients. When we added only tissue factor, i.e. made the results dependent upon phospholipids, the endogenous thrombin potential, the peak, and the velocity index were significantly higher and the time-to-peak was significantly shorter. This suggests that the plasma of cancer patients contained a higher activity of endogenous phospholipids and/or tissue factor which may be borne by microparticles.


CONCLUSIONS
This new simple methodology can demonstrate a procoagulant activity in the plasma of newly diagnosed cancer patients which can be explained by higher procoagulant phospholipids and tissue factor activity and thus, brings potentially useful information that current coagulation tests cannot provide.
Debaugnies
France
F
Laboratory of Hematology and Haemostasis, Université Libre de Bruxelles, Centre Hospitalier Universitaire Brugmann, Brussels, Belgium. fdebaugn@ulb.ac.be
Azerad
Marie-Agnès
MA
Noubouossié
Denis
D
Rozen
Laurence
L
Hemker
H Coenraad
HC
Corazza
F
F
Efira
André
A
Demulder
Anne
A
D001779:Blood Coagulation Factors / Q000032:analysis; D001780:Blood Coagulation Tests / Q000379:methods*; D016022:Case-Control Studies; D006801:Humans; D009369:Neoplasms / Q000097:blood*; D013917:Thrombin / Q000032:analysis* / Q000096:biosynthesis; D019851:Thrombophilia / Q000097:blood*
D001779:Blood Coagulation Factors; D013917:Thrombin