Phage display selection of miniprotein binders of the Estrogen Receptor.
Miniprotein phage display screening yields structured peptides with high affinity for the Estrogen Receptor (ER). Hits from apamin phage libraries feature a LXXLL motif specifically placed on the predefined miniprotein helical segment. The apamin scaffold also allows optimization of flanking amino acids to ensure an optimal ER binding affinity.
Phan
Trang
T
Chemical Genomics Centre of the Max Planck Society, Otto-Hahn Strasse 15, D-44227, Dortmund, Germany.
Nguyen
Hoang D
HD
Göksel
Hülya
H
Möcklinghoff
Sabine
S
Brunsveld
Luc
L
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D004220:Disulfides / Q000737:chemistry; D008969:Molecular Sequence Data; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry*; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011960:Receptors, Estrogen / Q000737:chemistry* / Q000378:metabolism
D004220:Disulfides; D019151:Peptide Library; D010455:Peptides; D011960:Receptors, Estrogen