Screening of tetrodotoxin in puffers using gas chromatography-mass spectrometry.
Tetrodotoxin (TTX), a toxic compound found in some puffers can cause death to humans through consumption. We have developed a simplified method for the screening of TTX in puffers using GC-MS. A puffer tissue of 0.5g was treated with 5mL of 0.1% acetic acid, followed by alkaline hydrolysis, LLE or liquid-liquid extraction and N-methyl-N-TMS-trifluoroacetamide derivatization. The developed method used only a small sample and solvent, simplified LLE and derivatization procedures and short chromatographic analysis (8.2min). All of these contribute to cost-saving, enhanced sample throughput and high sensitivity of the screening assay. The developed method was validated and proved to be within the acceptable range.
Man
Che Nin
CN
National Poison Centre, Universiti Sains Malaysia (USM), 11800 Penang, Malaysia. kaknin337@yahoo.com
Noor
Norjuliana Mohd
NM
Harn
Gam Lay
GL
Lajis
Razak
R
Mohamad
Samsur
S
D000818:Animals; D008401:Gas Chromatography-Mass Spectrometry / Q000191:economics / Q000379:methods*; D023721:Tetraodontiformes / Q000378:metabolism; D013779:Tetrodotoxin / Q000032:analysis* / Q000378:metabolism
D013779:Tetrodotoxin