[Expression of cannabinoid receptor I during mice skin incised wound healing course].
OBJECTIVE
To investigate the expression of cannabinoid receptor I (CB1R) during mice skin incised wound healing course and time-dependent changes of CB1R in wound age determination.


METHODS
The changes of CBIR expression in skin incised wound were detected by immunohistochemistry and Western blotting.


RESULTS
The control group showed a low expression of CB1R detected mainly in epidermis, hair follicles, sebaceous gland and dermomuscular layer. CB1R expression was undetectable in neutrophils in the wound specimens from 6h to 12h post-injury. CB1R positive cells were mostly mononuclear cells (MNCs) and fibroblastic cells (FBCs) from 1 d to 5 d post-injury. CB1R positive cells were mostly FBCs from 7 d to 14d post-injury. The ratio of the CB1R positive cells increased gradually in the wound specimens from 6 h to 3 d post-injury, reached peak level at 5 d, and then decreased gradually from 7d to 14 d post-injury. The positive bands of CB1R were observed in all time points of the wound healing course by Western blotting. The expression peak showed at 5 d post-injury.


CONCLUSIONS
CB1R is activated during the wound healing course. The expression of CB1R is found in mononuclear cells, which could be involved in inflammation reaction. CBIR is observed in fibroblastic cells, which could participate in the wound healing. CB1R may be a potentially useful marker for determination of wound healing age.
Zhao
Zhen-bin
ZB
School of Forensic Medicine, China Medical University, Shenyang 110001, China. zzb830220@163.com
Guan
Da-wei
DW
Liu
Wei-wei
WW
Wang
Tao
T
Fan
Yan-yan
YY
Cheng
Zi-hui
ZH
Zheng
Ji-long
JL
Hu
Geng-yi
GY
D000818:Animals; D015153:Blotting, Western; D004195:Disease Models, Animal; D005347:Fibroblasts / Q000378:metabolism; D049429:Forensic Pathology; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D009000:Monocytes / Q000378:metabolism; D011897:Random Allocation; D043884:Receptor, Cannabinoid, CB1 / Q000378:metabolism*; D012867:Skin / Q000293:injuries* / Q000378:metabolism*; D013194:Staining and Labeling; D013997:Time Factors; D014945:Wound Healing; D014947:Wounds and Injuries / Q000378:metabolism / Q000473:pathology
D043884:Receptor, Cannabinoid, CB1