Critical role for the chemokine receptor CXCR6 in NK cell-mediated antigen-specific memory of haptens and viruses.
Hepatic natural killer (NK) cells mediate antigen-specific contact hypersensitivity (CHS) in mice deficient in T cells and B cells. We report here that hepatic NK cells, but not splenic or naive NK cells, also developed specific memory of vaccines containing antigens from influenza, vesicular stomatitis virus (VSV) or human immunodeficiency virus type 1 (HIV-1). Adoptive transfer of virus-sensitized NK cells into naive recipient mice enhanced the survival of the mice after lethal challenge with the sensitizing virus but not after lethal challenge with a different virus. NK cell memory of haptens and viruses depended on CXCR6, a chemokine receptor on hepatic NK cells that was required for the persistence of memory NK cells but not for antigen recognition. Thus, hepatic NK cells can develop adaptive immunity to structurally diverse antigens, an activity that requires NK cell-expressed CXCR6.
Department of Pathology, Harvard Medical School, Boston, Massachusetts, USA.
Silke
S
Paust
Harvinder S
HS
Gill
Bao-Zhong
BZ
Wang
Michael P
MP
Flynn
E Ashley
EA
Moseman
Balimkiz
B
Senman
Marian
M
Szczepanik
Amalio
A
Telenti
Philip W
PW
Askenase
Richard W
RW
Compans
Ulrich H
UH
von Andrian
D056704:Adaptive Immunity / Q000276:immunology; D019264:Adoptive Transfer; D000818:Animals; D002469:Cell Separation; D002634:Chemotaxis, Leukocyte / Q000276:immunology; D003602:Cytotoxicity, Immunologic / Q000276:immunology; D005434:Flow Cytometry; D006241:Haptens / Q000276:immunology*; D007156:Immunologic Memory / Q000276:immunology*; D007694:Killer Cells, Natural / Q000276:immunology* / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000276:immunology; D016131:Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D054387:Receptors, CXCR / Q000276:immunology* / Q000378:metabolism; D000075744:Receptors, CXCR6; D014777:Virus Diseases / Q000276:immunology; D014780:Viruses / Q000276:immunology*
C482906:Cxcr6 protein, mouse; D006241:Haptens; D054387:Receptors, CXCR; D000075744:Receptors, CXCR6