The colorimetric detection and quantitation of total protein.
Protein quantification is an important step for handling protein samples for isolation and characterization; it is a prerequisite step before submitting proteins for chromatographic, electrophoretic, or immunochemical analysis and separation. Colorimetric methods are fast, simple, and not laborious. This unit describes a number of assays able to detect protein concentrations in the low microgram to milligram per milliliter ranges in a variety of formats.
Krohn
Randall I
RI
Pierce Biotechnology, Inc, Rockford, Illinois, USA.
D003124:Colorimetry; D007202:Indicators and Reagents / Q000737:chemistry*; D008832:Microchemistry / Q000379:methods; D011506:Proteins / Q000032:analysis* / Q000737:chemistry; D013053:Spectrophotometry; D014116:Toxicology / Q000379:methods
D007202:Indicators and Reagents; D011506:Proteins