Bioconversion of L-tyrosine to L-DOPA by a novel bacterium Bacillus sp. JPJ.
L-DOPA is an amino acid derivative and most potent drug used against Parkinson's disease, generally obtained from Mucuna pruriens seeds. In present communication, we have studied the in vitro production of L-DOPA from L-tyrosine by novel bacterium Bacillus sp. JPJ. This bacterium produced 99.4% of L-DOPA from L-tyrosine in buffer (pH 8) containing 1 mg ml(-1) cell mass incubated at 40°C for 60 min. The combination of CuSO(4) and L-ascorbic acid showed the inducing effect at concentrations of 0.06 and 0.04 mg ml(-1), respectively. The activated charcoal 2 mg ml(-1) was essential for maximum bioconversion of L-tyrosine to L-DOPA and the crude tyrosinase activity was 2.7 U mg(-1) of tyrosinase. Kinetic studies showed significant values of Y (p/s) (0.994), Q (s) (0.500) and q (s) (0.994) after optimization of the process. The production of L-DOPA was confirmed by analytical techniques such as HPTLC, HPLC and GC-MS. This is the first report on rapid and efficient production of L-DOPA from L-tyrosine by bacterial source which is more effective than the plant, fungal and yeast systems.
Department of Microbiology, Shivaji University, Vidyanagar, Kolhapur, 416004, India. shripad.surwase@gmail.com
Shripad N
SN
Surwase
Jyoti P
JP
Jadhav
D001205:Ascorbic Acid / Q000737:chemistry; D001407:Bacillus / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism*; D001711:Biotransformation; D019327:Copper Sulfate / Q000737:chemistry; D057075:Enzyme Assays; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D007980:Levodopa / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification; D014442:Monophenol Monooxygenase / Q000737:chemistry; D010802:Phylogeny; D014443:Tyrosine / Q000737:chemistry / Q000378:metabolism*
D014443:Tyrosine; D007980:Levodopa; D014442:Monophenol Monooxygenase; D019327:Copper Sulfate; D001205:Ascorbic Acid