Detection of HPV types and neutralizing antibodies in women with genital warts in Tianjin City, China.
The serum samples and corresponding cervical swabs were collected from 50 women with genital warts from Tianjin city, China. The neutralizing antibodies against HPV-16, -18, -58, -45, -6 and -11 in serum samples were tested by using pseudovirus-based neutralization assays and HPV DNAs in cervical swabs were also tested by using a typing kit that can detect 21 types of HPV. The results revealed that 36% (18/50) of sera were positive for type-specific neutralizing antibodies with a titer range of 160-2560, of which 22%(11/50), 12%(6/50), 10%(5/50), 4%(2/50), 4%(2/50) and 2%(1/50) were against HPVs -6, -16, -18, -58, -45 and -11, respectively. Additionally, 60% (30/50) of samples were HPV DNA-positive, in which the most common types detected were HPV-68(18%), HPV-16(14%), HPV-58(12%), HPV-33(8%) and HPV-6, HPV-11, HPV-18 and HPV-52 (6% each). The concordance between HPV DNA and corresponding neutralizing antibodies was 56% (28/50) with a significant difference (P<0.05). The full-length sequences of five HPV types (HPV -42, -52, -53, -58 and -68) were determined and exhibited 98%-100% identities with their reported genomes. The present data may have utility for investigating the natural history of HPV infection and promote the development of HPV vaccines.
Wu
Xue-ling
XL
Department of Cell Biology, National Institute for the Control of Pharmaceutical and Biological Products (NICPBP), Beijing 100050, China.
Zhang
Chun-tao
CT
Zhu
Xiao-ke
XK
Wang
You-chun
YC
D057134:Antibodies, Neutralizing / Q000097:blood*; D000914:Antibodies, Viral / Q000097:blood*; D001769:Blood / Q000276:immunology; D002584:Cervix Uteri / Q000821:virology; D002681:China; D016000:Cluster Analysis; D003218:Condylomata Acuminata / Q000821:virology*; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D005260:Female; D005838:Genotype; D006801:Humans; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D009500:Neutralization Tests / Q000379:methods; D027383:Papillomaviridae / Q000145:classification* / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D010802:Phylogeny; D015995:Prevalence; D017422:Sequence Analysis, DNA; D014773:Virology / Q000379:methods
D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D004279:DNA, Viral