Combination of intra-bone marrow-bone marrow transplantation and subcutaneous donor splenocyte injection diminishes risk of graft-versus-host disease and enhances survival rate.
The combination of allogeneic bone marrow transplantation (allo-BMT) and donor lymphocyte infusion (DLI) is a useful method for establishing donor chimerism and preventing a relapse of leukemia/lymphoma. However, there is a risk of inducing uncontrollable fatal graft-versus-host disease (GVHD). In fact, allo-BMT plus intravenous (IV)-DLI using donor splenocytes induces fatal GVHD in recipient mice. In this study, we examined the effects of the combination of intra-bone marrow (IBM)-BMT and the subcutaneous injection of donor splenocytes (SC-DLI) on the allo-BMT system. Recipient BALB/c mice were conditioned by sublethal irradiation (5 Gy), followed by IBM-BMT plus IV-DLI or SC-DLI in C57BL/6 mice. The IV-DLI group showed better engraftment of donor hemopoietic cells than the control group (without DLI) but showed fatal GVHD. The SC-DLI group, however, showed good reconstitution and mild GVHD. These results suggest that the combination of SC-DLI and IBM-BMT promotes the reconstitution of hemopoiesis and helps reduce the risk of GVHD.
Shi
Ming
M
Department of Stem Cell Disorders, Kansai Medical University, Moriguchi City, Osaka, Japan.
Adachi
Yasushi
Y
Cui
Yunze
Y
Li
Ming
M
Lian
Zhexiong
Z
Zhang
Yuming
Y
Yanai
Seiji
S
Shima
Chieko
C
Imai
Yuichiro
Y
Ikehara
Susumu
S
D000818:Animals; D001854:Bone Marrow Cells / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D016026:Bone Marrow Transplantation / Q000379:methods*; D003131:Combined Modality Therapy / Q000379:methods*; D006086:Graft vs Host Disease / Q000401:mortality / Q000473:pathology / Q000517:prevention & control*; D007275:Injections, Intravenous / Q000009:adverse effects; D007279:Injections, Subcutaneous / Q000379:methods*; D017710:Lymphocyte Transfusion / Q000379:methods*; D008214:Lymphocytes / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D012306:Risk; D013154:Spleen / Q000166:cytology / Q000378:metabolism; D015996:Survival Rate; D018183:Transplantation Chimera / Q000276:immunology; D014184:Transplantation, Homologous; D014916:Whole-Body Irradiation / Q000009:adverse effects