Evaluation of collagen in connective tissue walls of odontogenic cysts--a histochemical study.
BACKGROUND
The purpose of this study was to evaluate the nature of collagen in the connective tissue walls of odontogenic cysts, like the odontogenic keratocyst (OKC), dentigerous cyst and radicular cyst using picrosirius red stained sections. Furthermore, it was intended to assess if the capsular connective tissue can affect the nature of overlying epithelium, thus emphasizing the role of epithelial-mesenchymal interactions in biological behaviour of the cysts.


METHODS
The material for the study included 51 formalin-fixed paraffin-embedded tissue blocks (15 odontogenic keratocyst, 15 dentigerous cysts, 15 radicular cysts and four normal mucosa and two dental follicular tissue as controls), retrieved from the Department of Oral Pathology and Microbiology, MCODS, Manipal. Tissue blocks were sectioned at 5-μm thickness, stained with picrosirius red stain and observed with polarization and light microscopy.


RESULTS
Few sections of OKC and dentigerous cyst exhibited greenish-yellow birefringence in sub-epithelial region, whereas others showed a yellowish-orange birefringence under polarization microscopy. Most radicular cysts had yellowish-orange to orange birefringence. Shift in colour in case OKC and dentigerous cyst was attributed to the presence of inflammation in those sections. These regions also exhibited either a change in phenotype or thickness of overlying epithelium.


CONCLUSIONS
This technique can be used to study the nature of collagen fibres in odontogenic cyst walls. Further studies with an increased sample size and using various epithelial and mesenchymal markers and ssDNA antibodies should be carried out to confirm the effect of epithelial-mesenchymal interactions on the nature of epithelium of odontogenic cysts.
Vij
Ruchieka
R
Department of Oral Pathology & Microbiology, Institute of Dental Studies & Technologies, Modinagar, Uttar Pradesh, India. ruchieka@gmail.com
Vij
Hitesh
H
Rao
Nirmala N
NN
D001391:Azo Compounds; D003094:Collagen / Q000032:analysis*; D004396:Coloring Agents; D003238:Connective Tissue / Q000473:pathology; D003795:Dental Sac / Q000166:cytology; D003803:Dentigerous Cyst / Q000473:pathology; D004848:Epithelium / Q000473:pathology; D005881:Gingiva / Q000166:cytology; D006651:Histocytochemistry; D006801:Humans; D007249:Inflammation; D008859:Microscopy, Polarization; D009061:Mouth Mucosa / Q000166:cytology; D009807:Odontogenic Cysts / Q000473:pathology*; D011842:Radicular Cyst / Q000473:pathology
D001391:Azo Compounds; D004396:Coloring Agents; C009798:C.I. direct red 80; D003094:Collagen