The small ribosomal protein S12P gene rpsL as an efficient positive selection marker in allelic exchange mutation systems for Corynebacterium glutamicum.
We report that the mutant rpsL K43R in streptomycin-resistant and lysine-producing Corynebacterium glutamicum is responsible for streptomycin resistance. In addition, we describe its effective application in gene modification in C. glutamicum.
Laboratory of Immunobiology, School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University, Daegu, South Korea.
Il Kwon
IK
Kim
Weol Kyu
WK
Jeong
Seong Han
SH
Lim
In Kwan
IK
Hwang
Young Ho
YH
Kim
D019943:Amino Acid Substitution / Q000235:genetics; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D048230:Corynebacterium glutamicum / Q000187:drug effects / Q000235:genetics*; D024881:Drug Resistance, Bacterial; D018390:Gene Targeting; D005827:Genetics, Microbial / Q000379:methods*; D020125:Mutation, Missense; D011995:Recombination, Genetic; D012269:Ribosomal Proteins / Q000235:genetics*; D012641:Selection, Genetic; D013307:Streptomycin / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D012269:Ribosomal Proteins; C034212:ribosomal protein S12; D013307:Streptomycin