Monocytes from HTLV-1-infected patients are unable to fully mature into dendritic cells.
Human T-cell lymphotropic virus type 1 (HTLV-1) is a causative agent of adult T-cell leukemia and HTLV-1-associated myelopathy/tropical spastic paraparesis. HTLV-1-associated myelopathy/tropical spastic paraparesis is a chronic inflammatory disease characterized by loss of motor movement in response to spinal marrow cell destruction by T lymphocytes. To perform their cellular function, T cells need to be activated by antigen-presenting cells, such as dendritic cells (DCs). The aim of this work was to analyze DC differentiation and activation from monocytes of HTLV-1-infected individuals. We demonstrated that monocytes from HTLV-1-infected patients who had been stimulated to differentiate had an impaired loss of CD14 expression, expressed low levels of CD1a, and maintained secretion of tumor necrosis factor-α compared with monocytes from noninfected donors. We further evaluated DC activation by tumor necrosis factor-α. We observed that in response to activation, DCs that were derived from noninfected donors had an increase in the percentage of CD83(+), CD86(+), and human leukocyte antigen-DR(+) cells, whereas in DCs derived from HTLV-1-infected patients, the percentage of CD83(+), CD86(+), and human leukocyte antigen-DR(+) cells remained similar to that of nonactivated cells. Moreover, these cells had an impaired capacity to stimulate allogeneic T lymphocytes. We demonstrated that DC maturation was altered in HTLV-1-infected patients, which could contribute to the development of HTLV-1-associated diseases.
Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
Clarissa Rodrigues
CR
Nascimento
Marco Antonio
MA
Lima
Maria José
MJ
de Andrada Serpa
Otávio
O
Espindola
Ana Claudia Celestino
AC
Leite
Juliana
J
Echevarria-Lima
D000328:Adult; D000368:Aged; D002454:Cell Differentiation; D002469:Cell Separation; D016207:Cytokines / Q000096:biosynthesis; D006800:Deltaretrovirus Infections / Q000276:immunology*; D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D015368:Human T-lymphotropic virus 1; D006801:Humans; D007959:Lymphocyte Culture Test, Mixed; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000166:cytology* / Q000276:immunology*; D016133:Polymerase Chain Reaction; D055815:Young Adult
D016207:Cytokines