Effect of platelet-rich plasma on a rabbit model of nicotine-compromised bone healing.
OBJECTIVE
This study aimed to evaluate the effects of platelet-rich plasma (PRP) on nicotine-compromised bone healing.


METHODS
Fifteen adult New Zealand white rabbits were implanted with 1.5-g time-release nicotine pellets. Bilateral mandibular distraction osteogenesis was then performed. Autologous PRP was injected into 1 side of the distraction regenerate, whereas physiologic saline solution was injected into the contralateral side as a control. Five rabbits were killed on day 5 of active distraction, on day 11 of active distraction, and in week 2 of consolidation, respectively.


RESULTS
In the PRP the platelet enrichment was 14.63 ± 3.081-fold of that in whole blood. Plain radiography and micro-computed tomography assessment showed no significant difference between the PRP injection and control sides. Histologic examination showed more disorganized distraction tissue on the PRP injection side.


CONCLUSIONS
PRP injection at an early stage of active distraction does not significantly enhance bone healing in the nicotine-compromised rabbit model of mandibular lengthening.
Discipline of Oral & Maxillofacial Surgery, Faculty of Dentistry, The University of Hong Kong, Hong Kong, China.
Li
L
Ma
Li Wu
LW
Zheng
Mai Har
MH
Sham
Chun Kit
CK
Lam
Roger A
RA
Zwahlen
Lim Kwong
LK
Cheung
D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects; D003238:Connective Tissue / Q000187:drug effects / Q000473:pathology; D003692:Delayed-Action Preparations; D004195:Disease Models, Animal; D008334:Mandible / Q000000981:diagnostic imaging / Q000187:drug effects / Q000601:surgery*; D009538:Nicotine / Q000009:adverse effects*; D018722:Nicotinic Agonists / Q000009:adverse effects*; D010012:Osteogenesis / Q000187:drug effects; D019857:Osteogenesis, Distraction / Q000379:methods; D010027:Osteotomy / Q000379:methods; D010976:Platelet Count; D053657:Platelet-Rich Plasma / Q000502:physiology*; D011817:Rabbits; D013997:Time Factors; D053773:Transforming Growth Factor beta1 / Q000032:analysis; D014945:Wound Healing / Q000187:drug effects; D055114:X-Ray Microtomography
D003692:Delayed-Action Preparations; D018722:Nicotinic Agonists; D053773:Transforming Growth Factor beta1; D009538:Nicotine