Purification of plastocyanin and cytochrome c6 from plants, green algae, and cyanobacteria.
Plastocyanin and cytochrome c6 are widely distributed over the oxygen-evolving photosynthetic organisms. The two proteins are functionally equivalent, but strongly differ in their global electrostatic charge. In fact, they are acidic in eukaryotes, but either neutral or basic in cyanobacteria. Such a difference in their electrostatic features is a critical factor in designing the purification procedure, which must thus be modified and adapted accordingly. This chapter reports the methods for producing (including cell cultures), isolating, and purifying plastocyanin and cytochrome c6--which greatly differ in their isoelectric point--from a number of eukaryotic and prokaryotic organisms.
Instituto de Bioquímica Vegetal y Fotosíntesis, Universidad de Sevilla y Consejo Superior de Investigaciones Científicas, Sevilla, Spain.
José A
JA
Navarro
Manuel
M
Hervás
Miguel A
MA
De la Rosa
D049109:Cell Proliferation; D005591:Chemical Fractionation / Q000379:methods*; D011232:Chemical Precipitation; D000460:Chlorophyta / Q000737:chemistry* / Q000166:cytology / Q000201:enzymology; D002852:Chromatography, Ion Exchange; D000458:Cyanobacteria / Q000737:chemistry* / Q000166:cytology / Q000201:enzymology; D045365:Cytochromes c6 / Q000302:isolation & purification*; D004926:Escherichia coli / Q000737:chemistry / Q000166:cytology / Q000201:enzymology; D046937:Nostoc / Q000737:chemistry / Q000166:cytology / Q000201:enzymology; D018515:Plant Leaves / Q000737:chemistry / Q000166:cytology / Q000201:enzymology; D010970:Plastocyanin / Q000302:isolation & purification*; D018724:Spinacia oleracea / Q000737:chemistry* / Q000166:cytology / Q000201:enzymology; D046939:Synechocystis / Q000737:chemistry / Q000166:cytology / Q000201:enzymology
D045365:Cytochromes c6; D010970:Plastocyanin