Effects of 1,2,4,6-tetra-O-galloyl-β-D-glucose from P. emblica on HBsAg and HBeAg secretion in HepG2.2.15 cell culture.
A polyphenolic compound, 1,2,4,6-tetra-O-galloyl-β-D-glucose (1246TGG), was isolated from the traditional Chinese medicine Phyllanthus emblica L. (Euphorbiaceae) and assayed for its potential as an anti-hepatitis B virus (HBV) agent. The cytotoxicity of 1246TGG on HepG2.2.15 as well as HepG2 cells was determined by observing cytopathic effects, and the effects of 1246TGG on secretion of HBsAg and HBeAg in HepG2.2.15 cells were assayed by enzyme immunoassay. Results indicates that treatment with 1246TGG (6.25 μg/mL, 3.13 μg/mL), reduced both HBsAg and HBeAg levels in culture supernatant, yet the inhibitory effects tend to decline with the assay time. This study provides a basis for further investigation of the anti-HBV activity and possible mechanism of action of 1246TGG.
Xiang
Yang-Fei
YF
Biomedicine Research & Development Center, Jinan University, Guangzhou, 510630, China.
Ju
Huai-Qiang
HQ
Li
Shen
S
Zhang
Ying-Jun
YJ
Yang
Chong-Ren
CR
Wang
Yi-Fei
YF
D000998:Antiviral Agents / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D002460:Cell Line; D005707:Gallic Acid / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000494:pharmacology / Q000633:toxicity; D006514:Hepatitis B Surface Antigens / Q000378:metabolism*; D006513:Hepatitis B e Antigens / Q000378:metabolism*; D006515:Hepatitis B virus / Q000187:drug effects*; D022781:Hepatocytes / Q000187:drug effects / Q000821:virology*; D006801:Humans; D007124:Immunoenzyme Techniques; D009005:Monosaccharides / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D029842:Phyllanthus emblica / Q000737:chemistry*; D014779:Virus Replication / Q000187:drug effects
C520272:1,3,4,6-tetra-O-galloyl-beta-D-glucose; D000998:Antiviral Agents; D006514:Hepatitis B Surface Antigens; D006513:Hepatitis B e Antigens; D009005:Monosaccharides; D005707:Gallic Acid