[The diversity of extended gene haplotypes HLA-B*27 in Croatia].
In the present study the extended haplotypes of HLA-B*27 gene were analysed in the sample of 42 Croatian families. Peripheral blood (2 ml) was collected from all the individuals and genomic DNA was extracted using a commercial kit. The HLA alleles were determined using the method PCR sequence specific primers (PCR-SSP) while the microsatellite alleles were amplified using the method PCR-STR. Analysis of HLA-A-B*27-D6S2927-STR MICA-TNFalpha-DRB1 extended haplotypes demonstrated the strongest linkage disequilibrium between HLA-B*27 alleles and microsatellite alleles D6S2927-1 and STR_MICA-A4. Analysis indicated that the rare allele B*2730 is always present in the extended haplotype HLA-A3-B*2730-D6S2927-1-STR MICA-A4-TNFalpha-9-DRB1*16. The results of the present study will be applied in future studies of association between microsatellite alleles and spondyloarthropathies and contribute to a better understanding of peptide binding to HLA class I molecules, as well as other aspects of immune response.
Skifić
Marijana
M
Zavod za tipizaciju tkiva, Klinicki zavod za laboratorijsku dijagnostiku, Klinicki bolnicki centar Zagreb, Kispatićeva 12, 10000 Zagreb.
Stingl
Katarina
K
Zunec
Renata
R
Grubić
Zorana
Z
D000483:Alleles; D001483:Base Sequence; D017523:Croatia; D015796:HLA-B27 Antigen / Q000235:genetics*; D006239:Haplotypes; D006801:Humans; D015810:Linkage Disequilibrium; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction
D015796:HLA-B27 Antigen