Proteomic analysis of plasma proteins in Japanese semisuper centenarians.
We performed proteomic analysis of plasma proteins in Japanese semisuper centenarians (SSCs) (>105 years) and young controls (20-39 years), and found that 18 protein spots were altered in the plasma of SSCs. From peptide mass fingerprinting following in-gel digestion, it was demonstrated that paraoxonase/arylesterase 1 (PON 1) and apolipoprotein E were decreased, while haptoglobin β-chain, α(1)-microglobulin, and clusterin precursor were increased in SSCs. Interestingly, proteins related to oxidative stress, PON1, haptoglobin, α(1)-microglobulin, and clusterin, were altered in SSCs. These results suggest that systemic redox regulation is important for the longevity of SSCs. Overall, proteomics analysis is a powerful technique to search for useful biomarkers for future studies in gerontology and to characterize the individual proteins associated with successful aging of SSCs.
Research Team for Mechanism of Aging, Tokyo Metropolitan Institute of Gerontology, Tokyo 173-0015, Japan.
Yuri
Y
Miura
Yuji
Y
Sato
Yasumichi
Y
Arai
Yukiko
Y
Abe
Michiyo
M
Takayama
Tosifusa
T
Toda
Nobuyoshi
N
Hirose
Tamao
T
Endo
D000369:Aged, 80 and over; D000375:Aging / Q000378:metabolism*; D000510:Alpha-Globulins / Q000378:metabolism; D001057:Apolipoproteins E / Q000378:metabolism; D043303:Aryldialkylphosphatase / Q000378:metabolism; D044466:Asian People; D015415:Biomarkers / Q000097:blood; D001798:Blood Proteins / Q000378:metabolism*; D051152:Clusterin / Q000378:metabolism; D005260:Female; D006242:Haptoglobins / Q000378:metabolism; D006801:Humans; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000502:physiology; D010449:Peptide Mapping; D040901:Proteomics
D000510:Alpha-Globulins; D001057:Apolipoproteins E; D015415:Biomarkers; D001798:Blood Proteins; C495242:CLU protein, human; D051152:Clusterin; C485359:HP protein, human; D006242:Haptoglobins; C016001:alpha-1-microglobulin; D043303:Aryldialkylphosphatase; C468406:PON1 protein, human