Up-regulation of HIV-1 transduction in nondividing cells by double-strand DNA break-inducing agents.
Efficient HIV-1 transduction depends on a number of cellular co-factors. Cellular double-strand DNA break (DSB) repair proteins have been proposed, by ourselves and others, to be required for efficient HIV-1 transduction. Expression and/or activity of these DNA repair proteins can be induced by the introduction of DSBs into the host cell genome. HIV-1 transduction was up-regulated by treatment with DSB-inducing agents in both drug-arrested cells and differentiated neuronal cells. The presented data support the hypothesis that DSB repair proteins are involved in the early steps of the retroviral life-cycle.
Division of Infectious Diseases, Department of Medicine, Center for Human Virology, Thomas Jefferson University, Philadelphia, PA 19107, USA.
Johanna A
JA
Smith
René
R
Daniel
D000818:Animals; D002460:Cell Line; D053903:DNA Breaks, Double-Stranded; D045643:DNA Repair Enzymes / Q000378:metabolism*; D015870:Gene Expression; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D014161:Transduction, Genetic
D045643:DNA Repair Enzymes