High-throughput detection and sizing of individual low-index nanoparticles and viruses for pathogen identification.
Rapid, chip-scale, and cost-effective single particle detection of biological agents is of great importance to human health and national security. We report real-time, high-throughput detection and sizing of individual, low-index polystyrene nanoparticles and H1N1 virus. Our widefield, common path interferometer detects nanoparticles and viruses over a very large sensing area, orders of magnitude larger than competing techniques. We demonstrate nanoparticle detection and sizing down to 70 nm in diameter. We clearly size discriminate nanoparticles with diameters of 70, 100, 150, and 200 nm. We also demonstrate detection and size characterization of hundreds of individual H1N1 viruses in a single experiment.
Department of Biomedical Engineering, Boston University, 44 Cummington Street, Fourth Floor, Boston, Massachusetts 02215, United States.
G G
GG
Daaboul
A
A
Yurt
X
X
Zhang
G M
GM
Hwang
B B
BB
Goldberg
M S
MS
Ünlü
D015374:Biosensing Techniques / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D053118:Influenza A Virus, H1N1 Subtype / Q000302:isolation & purification* / Q000648:ultrastructure*; D007368:Interferometry / Q000295:instrumentation*; D053758:Nanoparticles; D049329:Nanostructures / Q000648:ultrastructure*; D010316:Particle Size