Novel protective agent GF-6 for skin wounds.
The aim of present investigation was the determination of specific pharmacological (wound healing) properties and the evaluation of possible irritative, allergenic and toxic effects of GF-6 - novel protective agent for skin mechanical and burn wounds. In mouse excisional wound model GF-6 (topically 0.1 ml per wound) exhibited the ability to accelerate scab rejection and full reepithelization in test wounds with no signs of bacterial contamination. GF-6 caused no irritation responses such as erythema, oedema, dotted hemorrhage, hyperkeratosis, hyperplasia, alopecia and scaling in 14-day acute dermal irritation test. As well no signs of skin hypersensitivity (itch, erythema, oedema), and histological alteration (ulceration, dotted hemorrhage, changed capillary lumen, damaged hair follicle) were observed in skin application test at all time points during the 14-day observation period, evidencing that GF-6 is non-allergenic. In subchronic dermal toxicity 90-day study of five-fold concentrated GF-6 no statistically significant changes in respiratory, circulatory, autonomic and central nervous system, somatomotor activity and behavior patterns were detected. The results of hematological study testified to no influence of the GF-6 90-day application on blood formed elements. Thus, GF-6 should be considered non-toxic, non-irritant, non-allergenic wound healing and wound protecting agent.
Mulkijanyan
K
K
I. Kutateladze Institute of Pharmacochemistry, Tbilisi, Georgia.
Novikova
J
J
Sulakvelidze
M
M
D000269:Adhesives / Q000008:administration & dosage* / Q000009:adverse effects; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000009:adverse effects; D001424:Bacterial Infections / Q000517:prevention & control; D003879:Dermatologic Agents / Q000008:administration & dosage* / Q000009:adverse effects; D004195:Disease Models, Animal; D006410:Hematopoiesis / Q000187:drug effects; D008099:Liver / Q000187:drug effects; D051379:Mice; D014945:Wound Healing / Q000187:drug effects*; D014947:Wounds and Injuries / Q000188:drug therapy* / Q000382:microbiology
D000269:Adhesives; D000900:Anti-Bacterial Agents; D003879:Dermatologic Agents