Lysobacter korlensis sp. nov. and Lysobacter bugurensis sp. nov., isolated from soil.
Two Gram-reaction-negative, rod-shaped, gliding, yellow-pigmented bacterial strains, designated ZLD-17(T) and ZLD-29(T), were isolated from arid soil samples collected from Xinjiang Province, north-west China, and subjected to analysis using a polyphasic taxonomic approach. Both novel strains required 1.0-2.0 % (w/v) sea salts for optimal growth. Phylogenetic analysis based on 16S rRNA gene sequences indicated that these two strains belong to the genus Lysobacter within the class Gammaproteobacteria. Strain ZLD-17(T) showed highest 16S rRNA gene sequence similarities to Lysobacter capsici KCTC 22007(T) (96.9 %), Lysobacter spongiicola DSM 21749(T) (96.8 %) and Lysobacter koreensis KCTC 12204(T) (96.8 %), whereas strain ZLD-29(T) showed highest sequence similarities to Lysobacter niastensis DSM 18481(T) (96.0 %) and Lysobacter enzymogenes DSM 2043(T) (95.9 %). 16S rRNA gene sequence similarity between ZLD-17(T) and ZLD-29(T) was 96.1 %. The DNA G+C contents of strains ZLD-17(T) and ZLD-29(T) were 67.9 and 68.2 mol%, respectively. The major cellular fatty acids of both strains were summed feature 3 (iso-C₁₅:₀ 2-OH and/or C₁₆:₁ω7c), iso-C₁₇:₁ω9c, iso-C₁₆:₀, C₁₆:₀ and iso-C₁₁:₀ 3-OH; their predominant isoprenoid quinone was Q-8 and their major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. Based on their phenotypic characteristics, phylogenetic position as determined by 16S rRNA gene sequence analysis and chemotaxonomic data, strains ZLD-17(T) ( = CCTCC AB 207174(T)  = KCTC 23076(T)) and ZLD-29(T) ( = CCTCC AB 207175(T) = KCTC 23077(T)) represent two novel species of the genus Lysobacter, for which the names Lysobacter korlensis sp. nov. and Lysobacter bugurensis sp. nov. are proposed, respectively.
College of Life Sciences, Wuhan University, Wuhan 430072, PR China.|College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
Lei
L
Zhang
College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
Juan
J
Bai
College of Life Sciences, Wuhan University, Wuhan 430072, PR China.
Yang
Y
Wang
State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, Northwest A&F University, Institute of Soil and Water Conservation, CAS & MWR, Yangling, Shaanxi 712100, PR China.
Gao-Lin
GL
Wu
College of Life Sciences, Wuhan University, Wuhan 430072, PR China.
Jun
J
Dai
College of Life Sciences, Wuhan University, Wuhan 430072, PR China.
Cheng-Xiang
CX
Fang
D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008124:Locomotion; D053528:Lysobacter / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D008969:Molecular Sequence Data; D010743:Phospholipids; D010802:Phylogeny; D010860:Pigments, Biological / Q000378:metabolism; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012492:Salts / Q000378:metabolism; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010743:Phospholipids; D010860:Pigments, Biological; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D012492:Salts