Enantioseparation of dansyl amino acids by HPLC on a monolithic column dynamically coated with a vancomycin derivative.
In this work a chiral stationary phase was prepared by dynamically coating a monolithic reversed-phase HPLC column with a vancomycin-derivative as chiral selector. A hydrophobic alkyl-chain was attached to the vancomycin molecule, providing the immobilization of the chiral selector on the reversed-phase material. Dansyl amino acids were chosen as model analytes for testing the separation power of the dynamically coated phase. All investigated compounds were separated into their enantiomers. Compared with a conventionally packed vancomycin-CSP, a reversal of the enantiomer elution order was obtained.
Pittler
Elfriede
E
Institute of Pharmaceutical Sciences, Department of Pharmaceutical Chemistry, Karl-Franzens-University, Universitätsplatz 1, A-8010 Graz, Austria.
Schmid
Martin G
MG
D000596:Amino Acids / Q000737:chemistry* / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D003619:Dansyl Compounds / Q000737:chemistry* / Q000302:isolation & purification*; D013237:Stereoisomerism; D014640:Vancomycin / Q000737:chemistry
D000596:Amino Acids; D003619:Dansyl Compounds; D014640:Vancomycin