Dual RMCE for efficient re-engineering of mouse mutant alleles.
We have developed dual recombinase-mediated cassette exchange (dRMCE) to efficiently re-engineer the thousands of available conditional alleles in mouse embryonic stem cells. dRMCE takes advantage of the wild-type loxP and FRT sites present in these conditional alleles and in many gene-trap lines. dRMCE is a scalable, flexible tool to introduce tags, reporters and mutant coding regions into an endogenous locus of interest in an easy and highly efficient manner.
Osterwalder
Marco
M
Developmental Genetics, Department of Biomedicine, University of Basel, Basel, Switzerland.
Galli
Antonella
A
Rosen
Barry
B
Skarnes
William C
WC
Zeller
Rolf
R
Lopez-Rios
Javier
J
D000483:Alleles; D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics; D002352:Carrier Proteins / Q000502:physiology; D053595:Embryonic Stem Cells / Q000378:metabolism*; D005818:Genetic Engineering / Q000379:methods*; D047908:Intracellular Signaling Peptides and Proteins; D051379:Mice; D009419:Nerve Tissue Proteins / Q000502:physiology; D009687:Nuclear Proteins / Q000502:physiology; D011401:Promoter Regions, Genetic; D045522:Recombinases / Q000502:physiology*; D011995:Recombination, Genetic; D051901:Smad4 Protein / Q000235:genetics
D051792:Basic Helix-Loop-Helix Proteins; D002352:Carrier Proteins; D047908:Intracellular Signaling Peptides and Proteins; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; D045522:Recombinases; D051901:Smad4 Protein; C498766:Hand2 protein, mouse; C490810:Smad4 protein, mouse; C483194:Zfp503 protein, mouse