Methods for distinguishing nitrosative and oxidative chemistry of reactive nitrogen oxide species derived from nitric oxide.
NO-derived intermediates formed under aerobic conditions may engage in complex chemical reactions with biologically important molecules. The outcomes of these reactions and their ultimate effect on biological systems depend on the selectivity of the species and the concentrations of different substances present and whether the reaction takes place in the gas or aqueous phase. In this unit conversion of two different compounds to fluorescent products is used to distinguish between oxidative and nitrosative chemistry of different reactive nitrogen oxide species.
National Cancer Institute, Bethesda, Maryland, USA.
D A
DA
Wink
S
S
Kim
A
A
Miles
D
D
Jourd'heuil
M B
MB
Grisham
D005453:Fluorescence; D009569:Nitric Oxide / Q000737:chemistry*; D010084:Oxidation-Reduction; D026361:Reactive Nitrogen Species / Q000737:chemistry*
D026361:Reactive Nitrogen Species; D009569:Nitric Oxide