[Significance and expression of FKHR and AKT after subarachnoid hemorrhage in rat brain cortex].
OBJECTIVE
To study the significance and expression of FKHR and AKT after subarachnoid hemorrhage (SAH) in rat brain cortex.


METHODS
Twenty-four rats were randomly divided into three groups: sham, SAH and SAH plus nimodipine (n=8 each). A reliable SAH model was established by double injections of blood into cistern magna in Wistar rats. The neurological scores were measured by Loeffler and the expressions of FKHR, P-FKHR, AKT and P-FKHR detected by Western blot.


RESULTS
Compared with sham group, the neurological score of SAH group obviously decreased (P < 0.05), the expression of FKHR became elevated in rat cortex (P < 0.01), the expression of AKT had no change and the expressions of P-AKT and P-FKHR obviously decreased (all P < 0.01). But the neurological score markedly increased (P < 0.01) and the expressions of P-AKT and P-FKHR became elevated (all P < 0.01) after administration of nimodipine.


CONCLUSIONS
Both P-AKT and P-FKHR are involved in the process of brain cortex damage induced by SAH. The protective effects of nimodipine on brain injury induced by SAH may be related to the elevated expressions of P-AKT and P-FKHR in brain cortex.
Department of Neurosurgery, First Hospital of Jilin University, Changchun 130021, China.
Chun-ming
CM
Miao
Qi
Q
Luo
Wei-wei
WW
Wang
Jin-song
JS
Kang
Guo-ying
GY
Shi
Hong-yan
HY
Li
Yong
Y
Zhang
D000818:Animals; D002540:Cerebral Cortex / Q000378:metabolism*; D005260:Female; D051858:Forkhead Transcription Factors / Q000378:metabolism*; D008297:Male; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013345:Subarachnoid Hemorrhage / Q000378:metabolism*; D000071161:Forkhead Box Protein O1
D051858:Forkhead Transcription Factors; D009419:Nerve Tissue Proteins; D051057:Proto-Oncogene Proteins c-akt; C074639:Foxo1 protein, rat; D000071161:Forkhead Box Protein O1