Cleavage specificities of the brother and sister proteases Lys-C and Lys-N.
Understanding of protease specificity is essential in proteomics strategies to correctly compare peptide fragmentation spectra with spectra predicted from data extracted from sequence databases. Here we evaluate the specificities of two proteolytic enzymes Lys-C and Lys-N, which cleave peptide-bonds at, respectively, the C- and N-terminal side of a lysine residue.
Biomolecular Mass Spectrometry and Proteomics Group, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands.
Reinout
R
Raijmakers
Pieter
P
Neerincx
Shabaz
S
Mohammed
Albert J R
AJ
Heck
D008239:Lysine / Q000737:chemistry*; D010447:Peptide Hydrolases / Q000737:chemistry / Q000378:metabolism*; D010455:Peptides / Q000737:chemistry*; D040901:Proteomics; D013379:Substrate Specificity
D010455:Peptides; D010447:Peptide Hydrolases; D008239:Lysine