Effect of feeding status on adjuvant arthritis severity, cachexia, and insulin sensitivity in male Lewis rats.
We studied the effect of food restriction, overfeeding, and normofeeding on cachexia, inflammatory and metabolic parameters, and insulin sensitivity in chronic adjuvant arthritis (AA) in rats. Food restriction during AA increased circulating ghrelin, corticosterone, decreased leptin, and ameliorated arthrogram score and systemic inflammation compared to normofeeding. Overfeeding worsened arthrogram score and systemic inflammation, and led to lipid accumulation in the liver, but not to alterations of adipokine and ghrelin plasma levels relative to normofeeding. Independently of feeding status, AA induced cachexia, in which modulation of mRNA expressions for appetite-regulating neuropeptides (NPY, AgRP, POMC, CART) in the arcuate nucleus (ARC) does not play a primary role. The overexpression of IL-1β mRNA in the ARC suggests its role in the mechanisms of impaired energy balance during AA under all feeding conditions. Normal HOMA index in all arthritic groups does not indicate the development of insulin resistance by feeding interventions in these rats.
Department of Normal, Pathological, and Clinical Physiology, Third Faculty of Medicine, Charles University in Prague, 120 00 Prague, Czech Republic.
Andrea
A
Stofkova
Blanka
B
Zelezna
Marianna
M
Romzova
Olga
O
Ulicna
Alexander
A
Kiss
Martina
M
Skurlova
Jana
J
Jurcovicova
D054392:Adipokines / Q000378:metabolism; D000305:Adrenal Cortex Hormones / Q000378:metabolism; D000821:Animal Feed; D000818:Animals; D001111:Arcuate Nucleus of Hypothalamus / Q000378:metabolism; D001168:Arthritis / Q000178:diet therapy / Q000628:therapy*; D002100:Cachexia / Q000378:metabolism*; D017024:Chemotherapy, Adjuvant / Q000379:methods*; D054439:Ghrelin / Q000378:metabolism; D007249:Inflammation; D007328:Insulin / Q000378:metabolism*; D020738:Leptin / Q000378:metabolism; D008297:Male; D009479:Neuropeptides / Q000737:chemistry; D051381:Rats; D011917:Rats, Inbred Lew
D054392:Adipokines; D000305:Adrenal Cortex Hormones; D054439:Ghrelin; D007328:Insulin; D020738:Leptin; D009479:Neuropeptides