[Mutational analysis of nm23-H1 gene in human lung cancer by polymerase chain reaction-single strand conformation polymorphism].
BACKGROUND
To investigate the mutation of nm23 gene and its correlation with development , progression and metastasis of human lung cancer.


METHODS
Polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) was employed for mutational analysis of the five exons of nm23-H1 gene in 45 surgically resected lung cancer and 7 normal pulmonary tissues.


RESULTS
In the examined DNA samples of pulmonary tissues , no homozygous loss of allele of nm23-H1 gene was observed. SSCP analysis revealed altered mobility of single stranded DNA of nm23-H1 exon 1 in one case of stage IIIB squamous cell pulmonary carcinoma with mediastinal lymphnode metastasis and malignant pleural effusion.


CONCLUSIONS
The frequency of nm23-H1 gene mutation in its coding region is rare in lung cancer. Mutation of nm23-H1 gene might play a role in the progression and metastasis of lung cancer.
Department of Thoracocardiac Surgery , The First University Hospital , West China University of Medical Sciences , Chengdu , Sichuan 610041 , P. R. China.
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L
Liu
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Q
Zhou
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Z
Sun
Y
Y
Qin
Y
Y
Shi
Z
Z
Sun