Methyl-beta-cyclodextrin induces mitochondrial cholesterol depletion and alters the mitochondrial structure and bioenergetics.
There is growing evidence of mitochondrial membrane raft-like microdomains that are involved in the apoptotic pathway. The aim of this study was to investigate the effect of methyl-beta-cyclodextrin (MβCD), being a well-known lipid microdomain disrupting agent and cholesterol chelator, on the structure and bioenergetics of rat liver mitochondria (RLM). We observed that MβCD decreases the function of RLM, induces changes in the mitochondrial configuration state and decreases the calcium chloride-induced swelling. These data suggest that disruption of mitochondrial raft-like microdomains by cholesterol efflux on one hand impairs mitochondrial bioenergetics, but on the other hand it protects the mitochondria from swelling.
Ziolkowski
Wieslaw
W
Department of Biochemistry, J. Sniadecki Academy of Physical Education and Sport, K. Gorskiego 1, 80-336 Gdansk, Poland. wiech@awf.gda.pl
Szkatula
Michał
M
Nurczyk
Artur
A
Wakabayashi
Takashi
T
Kaczor
Jan Jacek
JJ
Olek
Robert Antoni
RA
Knap
Narcyz
N
Antosiewicz
Jedrzej
J
Wieckowski
Mariusz Roman
MR
Wozniak
Michal
M
D000818:Animals; D002122:Calcium Chloride / Q000494:pharmacology; D002784:Cholesterol / Q000378:metabolism*; D004734:Energy Metabolism / Q000187:drug effects*; D021962:Membrane Microdomains / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D008928:Mitochondria / Q000737:chemistry / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism*; D009245:NADH Dehydrogenase / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D047392:beta-Cyclodextrins / Q000494:pharmacology*
D047392:beta-Cyclodextrins; C108732:methyl-beta-cyclodextrin; D002784:Cholesterol; D009245:NADH Dehydrogenase; D002122:Calcium Chloride