Construction of a functional IgG-binding luciferase fusion protein for the rapid detection of specific bacterial strains.
A fusion protein consisting of two IgG-binding domains of streptococcal protein G and firefly luciferase was constructed, and a simple and specific bioluminescent immunodetection system for bacterial strains was developed.
Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta, Midori-ku, Yokohama, 226-8501, Japan.
Makiko
M
Nakamura
Masayasu
M
Mie
Eiry
E
Kobatake
D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000302:isolation & purification*; D007074:Immunoglobulin G / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D008156:Luciferases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011485:Protein Binding; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013050:Spectrometry, Fluorescence / Q000379:methods*
D001426:Bacterial Proteins; C034284:IgG Fc-binding protein, Streptococcus; D007074:Immunoglobulin G; D011993:Recombinant Fusion Proteins; D008156:Luciferases