Completion of the full-length genome sequence of human parainfluenza virus types 4A and 4B: sequence analysis of the large protein genes and gene start, intergenic and end sequences.
We have already reported the nucleotide sequences of the NP, P/V, M, F and HN genes of human parainfluenza virus type 4A (hPIV-4A) and type 4B (hPIV-4B). Here, we have determined the sequences of the L protein genes as well as the gene start, intergenic and end sequences, thereby completing the full-length genome sequence of hPIV-4A and 4B. hPIV-4A and 4B have 17,052 and 17,304 nucleotides, respectively. The end sequence of hPIV-4, especially 4B, was extraordinarily long. In a comparison with members of the genus Rubulavirus, the hPIV-4 L proteins were closely related to those of mumps virus (MUV) and hPIV-2, less closely related to those of Menangle virus and Tioman virus, and more distantly related to those of Mapuera virus and porcine rubulavirus.
Department of Microbiology, Suzuka University of Medical Science Suzuka, Mie, Japan. komada@suzuka-u.ac.jp
Hiroshi
H
Komada
Mitsuo
M
Kawano
Atsuo
A
Uefuji
Morihiro
M
Ito
Masato
M
Tsurudome
Eri
E
Hatakeyama
Mai
M
Nakanishi
Shiho
S
Sakue
Chika
C
Joh
Eriko
E
Suzumura
Tatsuya
T
Tamaki
Toshifumi
T
Tomioka
Machiko
M
Nishio
Hideki
H
Tsumura
Jun
J
Uematsu
Hidetaka
H
Yamamoto
Myles
M
O'Brien
Hisanori
H
Bando
Yasuhiko
Y
Ito
D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D018388:Codon, Terminator / Q000235:genetics*; D021901:DNA, Intergenic / Q000235:genetics*; D007668:Kidney / Q000166:cytology; D008969:Molecular Sequence Data; D029482:Parainfluenza Virus 4, Human / Q000145:classification* / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*; D024363:Transcription Initiation Site
D018388:Codon, Terminator; D021901:DNA, Intergenic; D012367:RNA, Viral