Increased human pathogenic potential of Escherichia coli from polymicrobial urinary tract infections in comparison to isolates from monomicrobial culture samples.
The current diagnostic standard procedure outlined by the Health Protection Agency for urinary tract infections (UTIs) in clinical laboratories does not report bacteria isolated from samples containing three or more different bacterial species. As a result many UTIs go unreported and untreated, particularly in elderly patients, where polymicrobial UTI samples are especially prevalent. This study reports the presence of the major uropathogenic species in mixed culture urine samples from elderly patients, and of resistance to front-line antibiotics, with potentially increased levels of resistance to ciprofloxacin and trimethoprim. Most importantly, the study highlights that Escherichia coli present in polymicrobial UTI samples are statistically more invasive (P<0.001) in in vitro epithelial cell infection assays than those isolated from monomicrobial culture samples. In summary, the results of this study suggest that the current diagnostic standard procedure for polymicrobial UTI samples needs to be reassessed, and that E. coli present in polymicrobial UTI samples may pose an increased risk to human health.
Croxall
Gemma
G
Pathogen Research Group, Nottingham Trent University, Clifton Lane, Nottingham NG11 8NS, UK.
Weston
Vivienne
V
Nottingham University Hospitals, Nottingham, UK.
Joseph
Susan
S
Pathogen Research Group, Nottingham Trent University, Clifton Lane, Nottingham NG11 8NS, UK.
Manning
Georgina
G
Pathogen Research Group, Nottingham Trent University, Clifton Lane, Nottingham NG11 8NS, UK.
Cheetham
Phil
P
Pathogen Research Group, Nottingham Trent University, Clifton Lane, Nottingham NG11 8NS, UK.
McNally
Alan
A
Pathogen Research Group, Nottingham Trent University, Clifton Lane, Nottingham NG11 8NS, UK.
D000368:Aged; D000369:Aged, 80 and over; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001424:Bacterial Infections / Q000382:microbiology*; D002460:Cell Line; D004269:DNA, Bacterial / Q000235:genetics; D004847:Epithelial Cells / Q000382:microbiology; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests; D014552:Urinary Tract Infections / Q000382:microbiology*; D014774:Virulence; D001618:beta-Lactamases / Q000235:genetics
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D001618:beta-Lactamases