Survival of phosphate-solubilizing bacteria against DNA damaging agents.
Phosphate-solubilizing bacteria (PSBs) were isolated from different plant rhizosphere soils of various agroecological regions of India. These isolates showed synthesis of pyrroloquinoline quinone (PQQ), production of gluconic acid, and release of phosphorus from insoluble tricalcium phosphate. The bacterial isolates synthesizing PQQ also showed higher tolerance to ultraviolet C radiation and mitomycin C as compared to Escherichia coli but were less tolerant than Deinococcus radiodurans. Unlike E. coli, PSB isolates showed higher tolerance to DNA damage when grown in the absence of inorganic phosphate. Higher tolerance to ultraviolet C radiation and oxidative stress in these PSBs grown under PQQ synthesis inducible conditions, namely phosphate starvation, might suggest the possible additional role of this redox cofactor in the survival of these isolates under extreme abiotic stress conditions.
Shrivastava
Manoj
M
Bhabha Atomic Research Centre, Mumbai, India. shriman31@rediffmail.com
Rajpurohit
Yogendra S
YS
Misra
Hari S
HS
D'Souza
S F
SF
D016956:Burkholderia cepacia / Q000145:classification / Q000302:isolation & purification / Q000502:physiology*; D002130:Calcium Phosphates / Q000378:metabolism; D002374:Catalase; D004249:DNA Damage; D004754:Enterobacter / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D004755:Enterobacteriaceae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D005942:Gluconates / Q000378:metabolism; D007194:India; D016685:Mitomycin / Q000494:pharmacology; D018384:Oxidative Stress; D045542:PQQ Cofactor / Q000096:biosynthesis*; D020636:Pantoea / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D010710:Phosphates / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D011513:Proteus mirabilis / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D044204:Pseudomonas oleovorans / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D011836:Radiation Tolerance; D058441:Rhizosphere; D012988:Soil Microbiology; D014466:Ultraviolet Rays
D002130:Calcium Phosphates; D005942:Gluconates; D010710:Phosphates; D016685:Mitomycin; D045542:PQQ Cofactor; D002374:Catalase; C018392:tricalcium phosphate; C030691:gluconic acid