Stamp wound assay for studying coupled cell migration and cell debris clearance.
A new method for studying wound healing under realistic conditions in vitro was developed. The method involves creating defined patterns of damaged cell debris with poly(dimethyl)siloxane (PDMS) stamping. This novel assay permitted the quantification of wound healing rates in the presence of cell debris. Experimental results with this assay suggest that cell migration in the presence of cell debris is a two step process requiring (1) non-muscle myosin II-dependent cell clearance followed by (2) cell migration into newly cleared wound areas. The novel stamp wound assay allows the study of coupled cell migration and debris clearance and is a more realistic wound healing assay in vitro.
Department of Chemical Engineering, University of Florida, Gainesville, Florida 32611, United States.
Jiyeon
J
Lee
Yu-Lin
YL
Wang
Fan
F
Ren
Tanmay P
TP
Lele
D016923:Cell Death / Q000187:drug effects; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D018914:Cells, Immobilized / Q000166:cytology / Q000187:drug effects; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects; D006576:Heterocyclic Compounds, 4 or More Rings / Q000494:pharmacology; D006801:Humans; D024462:Myosin Type II / Q000037:antagonists & inhibitors; D013997:Time Factors; D014945:Wound Healing / Q000187:drug effects
D004129:Dimethylpolysiloxanes; D006576:Heterocyclic Compounds, 4 or More Rings; C472645:blebbistatin; C013830:baysilon; D024462:Myosin Type II