Simultaneous production of 1,3-dihydroxyacetone and xylitol from glycerol and xylose using a nanoparticle-supported multi-enzyme system with in situ cofactor regeneration.
Cofactor-dependent biotransformations often require consumption of a secondary substrate for cofactor regeneration. Alternatively, two synthetic reactions may be coupled together through cofactor regeneration cycles. Simultaneous production of value-added products from glycerol and xylose was realized in this work through an enzymatic NAD(H) regeneration cycle involving two enzymes. Glycerol dehydrogenase (GDH) catalyzed the production of 1,3-dihydroxyacetone (DHA) from glycerol, while xylose reductase (XR) enabled the reduction of xylose to xylitol using the protons released from glycerol. Both enzymes were immobilized with P(MMA-EDMA-MAA) nanoparticles. Interestingly, the immobilized multi-enzyme system showed much improved productivity and stability as compared to native enzymes, such that the total turnover number (TTN) reached 82 for cofactor regeneration while the yield reached 160g/g-immobilized GDH for DHA production.
National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China.
Ying
Y
Zhang
Fei
F
Gao
Song-Ping
SP
Zhang
Zhi-Guo
ZG
Su
Guang-Hui
GH
Ma
Ping
P
Wang
D000449:Aldehyde Reductase / Q000378:metabolism; D055162:Biocatalysis; D002021:Buffers; D003067:Coenzymes / Q000378:metabolism*; D003470:Culture Media; D004098:Dihydroxyacetone / Q000138:chemical synthesis*; D004795:Enzyme Stability; D005990:Glycerol / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008954:Models, Biological; D009097:Multienzyme Complexes / Q000378:metabolism*; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D059027:Recycling; D013401:Sugar Alcohol Dehydrogenases / Q000378:metabolism; D014993:Xylitol / Q000138:chemical synthesis*; D014994:Xylose / Q000378:metabolism*
D002021:Buffers; D003067:Coenzymes; D003470:Culture Media; D009097:Multienzyme Complexes; D014994:Xylose; D013401:Sugar Alcohol Dehydrogenases; D000449:Aldehyde Reductase; C020672:glycerol dehydrogenase; D004098:Dihydroxyacetone; D005990:Glycerol; D014993:Xylitol