Quantifying the amount and activity of Rubisco in leaves.
The CO2-fixing enzyme Rubisco plays a crucial biological role as a primary determinant of both plant yield and the response of the biosphere to global change. Here, we describe techniques for measuring the amount and activity of Rubisco in higher plants. To accommodate a range of experimental capabilities, we describe basic radioisotopic methods as well as non-radioactive techniques. The required calculations are included. We discuss problems that commonly arise during the extraction and assay of Rubisco.
Kubien
David S
DS
Department of Biology, University of New Brunswick, Fredericton, NB, Canada.
Brown
Christopher M
CM
Kane
Heather J
HJ
D015153:Blotting, Western; D002245:Carbon Dioxide / Q000378:metabolism; D002845:Chromatography; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation; D057075:Enzyme Assays / Q000379:methods*; D010428:Pentosephosphates / Q000378:metabolism; D018515:Plant Leaves / Q000201:enzymology*; D010944:Plants / Q000201:enzymology*; D012273:Ribulose-Bisphosphate Carboxylase / Q000032:analysis* / Q000378:metabolism*; D017693:Sodium Bicarbonate / Q000378:metabolism; D013053:Spectrophotometry; D013402:Sugar Alcohols / Q000378:metabolism
D010428:Pentosephosphates; D013402:Sugar Alcohols; D002245:Carbon Dioxide; C032687:2-carboxyarabinitol 1,5-biphosphate; D017693:Sodium Bicarbonate; D012273:Ribulose-Bisphosphate Carboxylase