Defects and rescue of the minor salivary glands in Eda pathway mutants.
Despite their importance to oral health, the mechanisms of minor salivary gland (SG) development are largely unexplored. Here we present in vivo and in vitro analyses of developing minor SGs in wild type and mutant mice. Eda, Shh and Fgf signalling pathway genes are expressed in these glands from an early stage of development. Developing minor SGs are absent in Eda pathway mutant embryos, and these mice exhibit a dysplastic circumvallate papilla with disrupted Shh expression. Supplementation of Eda pathway mutant minor SG explants with recombinant EDA rescues minor SG induction. Supplementation with Fgf8 or Shh, previously reported targets of Eda signalling, leads to induction of gland like structures in a few cases, but these fail to develop into minor SGs.
Department of Craniofacial Development, King's College London Dental Institute, London SE1 9RT, UK.
K L
KL
Wells
C
C
Mou
D J
DJ
Headon
A S
AS
Tucker
D000818:Animals; D017931:DNA Primers / Q000235:genetics; D053331:Ectodysplasins / Q000235:genetics / Q000378:metabolism*; D051524:Fibroblast Growth Factor 8 / Q000378:metabolism / Q000494:pharmacology; D005838:Genotype; D053823:Hedgehog Proteins / Q000378:metabolism / Q000494:pharmacology; D006652:Histological Techniques; D017403:In Situ Hybridization; D051379:Mice; D008817:Mice, Mutant Strains; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000378:metabolism / Q000494:pharmacology*; D012470:Salivary Glands, Minor / Q000187:drug effects / Q000196:embryology*; D015398:Signal Transduction / Q000502:physiology*
D017931:DNA Primers; D053331:Ectodysplasins; C506019:Eda protein, mouse; C496457:Fgf8 protein, mouse; D053823:Hedgehog Proteins; D011994:Recombinant Proteins; C465570:Shh protein, mouse; D051524:Fibroblast Growth Factor 8