Karyotypic evolution in family Hipposideridae (Chiroptera, Mammalia) revealed by comparative chromosome painting, G- and C-banding.
Comparing to its sister-family (Rhinolophidae), Hipposideridae was less studied by cytogenetic approaches. Only a few high-resolution G-banded karyotypes have been reported so far, and most of the conclusions on the karyotypic evolution in Hipposideridae were based on conventional Giemsa-staining. In this study, we applied comparative chromosome painting, a method of choice for genome-wide comparison at the molecular level, and G- and C-banding to establish comparative map between five hipposiderid species from China, using a whole set of chromosome-specific painting probes from one of them (Aselliscus stoliczkanus). G-band and C-band comparisons between homologous segments defined by chromosome painting revealed that Robertsonian translocations, paracentric inversions and heterochromatin addition could be the main mechanism of chromosome evolution in Hipposideridae. Comparative analysis of the conserved chromosomal segments among five hipposiderid species and outgroup species suggests that bi-armed chromosomes should be included into the ancestral karyotype of Hipposideridae, which was previously believed to be exclusively composed of acrocentric chromosomes.
Mao
Xiu-Guang
XG
Kunming Institute of Zoology, the Chinese Academy of Sciences, Kunming Yunnan 650223, China.
Wang
Jin-Huan
JH
Su
Wei-Ting
WT
Wang
Ying-Xiang
YX
Yang
Feng-Tang
FT
Nie
Wen-Hui
WH
D000818:Animals; D005075:Biological Evolution; D002685:Chiroptera / Q000235:genetics*; D002871:Chromosome Banding / Q000379:methods*; D020223:Chromosome Painting / Q000379:methods*; D033481:Chromosomes, Mammalian / Q000235:genetics*; D007621:Karyotyping