Inducible nitric oxide synthase expression.
This unit contains three protocols that can be used to determine iNOS expression in mouse macrophage-like cells, RAW 264.7, by measuring end product, protein, and mRNA. A bacterial product, lipopolysaccharide (LPS), stimulates iNOS expression in RAW 264.7 cells.
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Kenneth S. Warren Laboratories, Tarrytown, New York, USA.
D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D002460:Cell Line; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D051379:Mice; D052247:Nitric Oxide Synthase Type II / Q000235:genetics / Q000378:metabolism*; D009573:Nitrites / Q000378:metabolism*; D009690:Nucleic Acid Conformation; D012333:RNA, Messenger / Q000235:genetics
D009573:Nitrites; D012333:RNA, Messenger; D052247:Nitric Oxide Synthase Type II