Mitochondrial genome of the cotton bollworm Helicoverpa armigera (Lepidoptera: Noctuidae) and comparison with other Lepidopterans.
We present the complete sequence of the mitochondrial genome (mitogenome) of the cotton bollworm Helicoverpa armigera. The 15,347-bp mitogenome of H. armigera was arranged in the same order described for all other sequenced lepidopterans, which differs from the most common type found in insects, due to the movement of trnM to a position 5'-upstream of trnI. The gene overlap in the H. armigera mitogenome is totally 23 bp in six locations. The H. armigera mitogenome has a total of 175 bp of intergenic spacer sequences spread over 14 regions ranging in size from 1 to 45 bp. The nucleotide composition of the whole mitogenome of H. armigera is highly A+T biased, accounting for 80.97%, with a slightly positive AT skewness and negative GC skewness, indicating the occurrence of more A than T, C more than G. The protein-encoding genes have typical mitochondrial start codons, except for cox1, which contains the unusual CGA. The cox1, cox2, and nad4 genes have incomplete stop codons (T). The lrRNA and srRNA genes are 1395 and 794-bp long, respectively. All tRNAs have a typical cloverleaf structure of mitochondrial tRNAs, except for trnS1(AGN), the dihydrouridine arm of which could not form a stable stem-loop structure. The H. armigera A+T-rich region contains a conserved structure combining the motif ATAGA and a 19-bp poly-T stretch, but absence of the 9-bp poly-A element upstream of trnM.
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, People's Republic of China.
Jiao
J
Yin
Gui-Yun
GY
Hong
Ai-Min
AM
Wang
Ya-Zhong
YZ
Cao
Zhao-Jun
ZJ
Wei
D000818:Animals; D001482:Base Composition; D001483:Base Sequence; D004272:DNA, Mitochondrial / Q000235:genetics; D023061:Gene Order; D050259:Genes, Mitochondrial / Q000235:genetics; D049750:Genome, Insect; D054629:Genome, Mitochondrial; D003368:Gossypium / Q000469:parasitology*; D007915:Lepidoptera / Q000145:classification* / Q000235:genetics; D008969:Molecular Sequence Data; D009036:Moths / Q000235:genetics*; D012343:RNA, Transfer / Q000235:genetics; D017422:Sequence Analysis, DNA; D013045:Species Specificity
D004272:DNA, Mitochondrial; D012343:RNA, Transfer