Engineering a collagen matrix that replicates the biological properties of native extracellular matrix.
In this study, we aimed to replicate the function of native tissues that can be used in tissue engineering and regenerative medicine. The key to such replication is the preparation of an artificial collagen matrix that possesses a structure resembling that of the extracellular matrix. We, therefore, prepared a collagen matrix by fibrillogenesis in a NaCl/Na(2)HPO(4) aqueous solution using a dialysis cassette and investigated its biological behavior in vitro and in vivo. The in vitro cell adhesion and proliferation did not show any significant differences. The degradation rate in the living body could be controlled according to the preparation condition, where the collagen matrix with high water content (F-collagen matrix, >98%) showed fast degradation and collagen matrix with lower water content (T-collagen matrix, >80%) showed no degradation for 8 weeks. The degradation did not affect the inflammatory response at all and relatively faster wound healing response was observed. Comparing this result with that of collagen gel and decellularized cornea, it can be concluded that the structural factor is very important and no cell abnormal behavior would be observed for quaternary structured collagen matrix.
Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda-ku, Tokyo 101-0062, Japan.
Kwangwoo
K
Nam
Yuuki
Y
Sakai
Seiichi
S
Funamoto
Tsuyoshi
T
Kimura
Akio
A
Kishida
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000378:metabolism; D002460:Cell Line; D003094:Collagen / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism; D003315:Cornea / Q000166:cytology*; D005109:Extracellular Matrix / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology; D005782:Gels / Q000737:chemistry* / Q000378:metabolism; D007249:Inflammation / Q000276:immunology; D008297:Male; D051379:Mice; D051381:Rats; D017208:Rats, Wistar; D023822:Tissue Engineering / Q000379:methods; D014945:Wound Healing
D001672:Biocompatible Materials; D005782:Gels; D003094:Collagen