Vestibular tributaries to the vein of the vestibular aqueduct.
CONCLUSIONS
The vein of the vestibular aqueduct drains blood from areas extensively lined by vestibular dark cells (VDCs). A possible involvement in the pathogenesis of an impaired endolymphatic homeostasis can be envisioned at the level of the dark cells area.


OBJECTIVE
The aim of this study was to investigate the vascular relationship between the vein of the vestibular aqueduct and the vestibular apparatus, with focus on the VDCs.


METHODS
Sixteen male Wistar rats were divided into groups of 6 and 10. In the first group, 2 μm thick sections including the vein of the vestibular aqueduct, utricle, and crista ampullaris of the lateral ampulla were examined by light microscopy and computer-generated three-dimensional imaging. In the second group, ultrathin sections including venules and VDCs were examined by transmission electron microscopy.


RESULTS
A microvascular network was observed in close relation to the VDCs in the utricle and the crista ampullaris of the lateral semicircular canal in the vestibular apparatus. One major vein emanated from these networks, which emptied into the vein of the vestibular aqueduct. Veins draining the saccule and the common crus of the superior and posterior semicircular canals were likewise observed to merge with the vein of the vestibular aqueduct.
Department of Biomedical Sciences, Panum Institute, University of Copenhagen, Denmark. marsner@me.com
Jesper Marsner
JM
Hansen
Klaus
K
Qvortrup
Morten
M
Friis
D000818:Animals; D004710:Endolymph / Q000502:physiology*; D006706:Homeostasis; D021621:Imaging, Three-Dimensional; D008297:Male; D046529:Microscopy, Electron, Transmission; D055806:Microvessels / Q000033:anatomy & histology; D051381:Rats; D017208:Rats, Wistar; D012016:Reference Values; D012444:Saccule and Utricle / Q000098:blood supply; D012665:Semicircular Canals / Q000098:blood supply; D054776:Semicircular Ducts / Q000098:blood supply; D014680:Veins / Q000033:anatomy & histology; D014723:Vestibular Aqueduct / Q000098:blood supply*; D014722:Vestibule, Labyrinth / Q000098:blood supply*