Improvement of galactose induction system in Saccharomyces cerevisiae.
Here we report a significant enhancement of galactose response without altering the characteristics of glucose repression. To improve the galactose response, we fabricated transgenic yeasts harboring HIS3pro-GAL1, HIS3pro-GAL2 and GAL10pro-GAL4, and evaluated the synergistic effects of these three genes by immunoblot and flow cytometry analyses.
Matsuyama
Takashi
T
Matsuyama Research Group, Toyota central R&D Labs. Inc., 41-1 Nagakute-yokomichi, Nagakute-cho, Aichi 480-1192, Japan. e1215@mosk.tytlabs.co.jp
Yamanishi
Mamoru
M
Takahashi
Haruo
H
D003470:Culture Media; D005690:Galactose / Q000235:genetics / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D005947:Glucose / Q000378:metabolism; D011401:Promoter Regions, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014158:Transcription, Genetic
D003470:Culture Media; D005947:Glucose; D005690:Galactose