Different length (DL) qPCR for quantification of cell killing by UV-induced DNA damage.
We describe the different length (DL) qPCR method for quantification of UV induced DNA damage in cell killing. The principle of DL qPCR is that DNA damage inhibits PCR. Applications with different lengths can therefore be used to detect different levels of UV-induced DNA damage. The assay was evaluated on three strains of Escherichia coli exposed to varying levels of ultraviolet (UV) radiation. We show that DL qPCR sensitivity and reproducibility are within the range of practical application to detect the effect of UV cell killing.
Rudi
Knut
K
Hedmark University College, Lærerskolealleen 1, 2418 Elverum, Norway. knut.rudi@hihm.no
Hagen
Irina
I
Johnsrud
Bente Carina
BC
Skjefstad
Guro
G
Tryland
Ingun
I
D001681:Biological Assay / Q000379:methods; D004249:DNA Damage; D004926:Escherichia coli; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D014466:Ultraviolet Rays / Q000009:adverse effects*