Characterization of novel alleles of toxin co-regulated pilus A gene (tcpA) from environmental isolates of Vibrio cholerae.
Vibrio cholerae is causative agent of life threatening diarrheal disease, cholera. The toxin co-regulated pilus (TCP) is a critical colonization factor of V. cholerae and it also serves as receptor for CTXФ. In this study, we describe nucleotide sequence of four novel alleles of tcpA gene from toxigenic and non-toxigenic V. cholerae isolated from environmental sources. The phylogenetic analysis of tcpA revealed that it is related to tcpA of newly emerged O1 strain and unrelated to tcpA of wild type (classical and El Tor strains). All strains showed variant tcpA and also harbored intact Vibrio Pathogenicity Island (VPI). The expression of all variant alleles was demonstrated by RT-PCR.
Department of Molecular Microbiology, Cholera and Environmental Microbiology Laboratory, Rajiv Gandhi Centre for Biotechnology, Trivandrum, Kerala, India.
Praveen
P
Kumar
Anuja
A
Thulaseedharan
Gautam
G
Chowdhury
Thandavarayan
T
Ramamurthy
Sabu
S
Thomas
D000483:Alleles; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004783:Environmental Microbiology; D036781:Fimbriae Proteins / Q000235:genetics*; D020869:Gene Expression Profiling; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D011110:Polymorphism, Genetic; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014734:Vibrio cholerae / Q000235:genetics* / Q000302:isolation & purification; D037521:Virulence Factors / Q000235:genetics*
D004269:DNA, Bacterial; C085807:TcpA protein, Vibrio cholerae; D037521:Virulence Factors; D036781:Fimbriae Proteins