Wound healing following regenerative procedures in furcation degree III defects: histomorphometric outcomes.
Degree III furcation involvements were surgically created at four first molars in each of three monkeys. Following 6 weeks of healing, full-thickness flaps were elevated. Following 24% EDTA gel conditioning, the defects were treated with one of the following: (1) enamel matrix proteins (EMD), (2) guided tissue regeneration (GTR) or (3) a combination EMD and GTR. The control defects did not receive any treatment. After 5 months of healing, the animals were sacrificed. Three 8 μm thick histological central sections, 100 μm apart, were used for histomorphometric analysis in six zones of each tooth either within the furcation area or on the pristine external surface of the root. In all specimens, new cementum with inserting collagen fibres was formed. Following GTR or GTR + EMD, cementum was formed up to and including the furcation fornix indicating complete regeneration on the defect periphery. Periodontal ligament fibres were less in all four modalities compared to pristine tissues. In the teeth treated with GTR and GTR + EMD a higher volume of bone and periodontal ligament tissues was observed compared to EMD. After 5 months of healing, regenerated tissues presented quantitative differences from the pristine tissues. In the two modalities where GTR alone or combined with EMD was used, the regenerated tissues differed in quantity from the EMD-treated sites.
Department of Periodontology, UCL Eastman Dental Institute for Oral Health Care Sciences University of London, 256 Gray's Inn Road, London, WC1X 8LD, UK.
Nikolaos D
ND
Gkranias
Filippo
F
Graziani
Anton
A
Sculean
Nikolaos
N
Donos
D020341:Absorbable Implants; D000539:Alveolar Process / Q000473:pathology; D000818:Animals; D001853:Bone Marrow / Q000473:pathology; D025021:Cementogenesis / Q000502:physiology; D002614:Chelating Agents / Q000627:therapeutic use; D003094:Collagen; D003739:Dental Cementum / Q000473:pathology; D003746:Dental Enamel Proteins / Q000627:therapeutic use; D012534:Dental Scaling / Q000379:methods; D004492:Edetic Acid / Q000627:therapeutic use; D017823:Furcation Defects / Q000145:classification / Q000473:pathology / Q000601:surgery*; D016557:Guided Tissue Regeneration, Periodontal / Q000379:methods*; D007091:Image Processing, Computer-Assisted / Q000379:methods; D008252:Macaca fascicularis; D008297:Male; D008336:Mandibular Diseases / Q000473:pathology / Q000601:surgery; D008567:Membranes, Artificial; D008963:Molar / Q000473:pathology / Q000601:surgery; D010513:Periodontal Ligament / Q000473:pathology; D011098:Polyglactin 910; D016745:Root Planing / Q000379:methods; D013524:Surgical Flaps; D014092:Tooth Root / Q000473:pathology; D016896:Treatment Outcome; D014945:Wound Healing / Q000502:physiology
D002614:Chelating Agents; D003746:Dental Enamel Proteins; D008567:Membranes, Artificial; C094810:Resolut; C109069:enamel matrix proteins; D011098:Polyglactin 910; D003094:Collagen; D004492:Edetic Acid