Antimutagenic and antioxidant characteristics of Chukrasia tabularis A Juss extracts.
The current study aims to evaluate the antioxidative and antimutagenic activities of methanol extract and different fractions of Chukrasia tabularis leaves. The antioxidative potential was evaluated using 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) cation and superoxide anion radical-scavenging assay. The antimutagenic potential was evaluated against direct-acting mutagens, 4-nitro-o-phenylenediamine and sodium azide; and S9-dependent mutagen, 2-aminofluorene in TA98 and TA100 strains of Salmonella typhimurium using Ames assay. It has been found that methanol extract and its fractions were more efficient against S9-dependent mutagen in pre-incubation mode of treatment as compared to direct-acting mutagens in both the strains. Methanol extract and its fractions also exhibited strong radical-scavenging potential. High-performance liquid chromatography (HPLC) analysis of methanol extract showed the presence of gallic acid, epicatechin, 7-hydroxycoumarin, and rutin. From the study, it could be concluded that antioxidative and antimutagenic activity of methanol extract and its fractions was related to the synergistic interactions among different chemical compounds.
Kaur
Rajbir
R
Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar, Punjab, India.
Sharma
Upendra
U
Singh
Bikram
B
Arora
Saroj
S
D000818:Animals; D016587:Antimutagenic Agents / Q000737:chemistry / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000187:drug effects; D004249:DNA Damage / Q000187:drug effects; D004336:Drug Antagonism; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000494:pharmacology; D008297:Male; D029582:Meliaceae / Q000737:chemistry*; D008862:Microsomes, Liver; D009153:Mutagens / Q000633:toxicity; D018515:Plant Leaves / Q000737:chemistry; D051381:Rats; D000091243:Ribosomal Protein S9; D012269:Ribosomal Proteins / Q000187:drug effects / Q000378:metabolism; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics
D016587:Antimutagenic Agents; D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D016166:Free Radical Scavengers; D009153:Mutagens; D000091243:Ribosomal Protein S9; D012269:Ribosomal Proteins; D004247:DNA