Complete genomic sequence of transmissible gastroenteritis virus TS and 3' end sequence characterization following cell culture.
The complete genome sequence of transmissible Gastroenteritis virus (TGEV) strain TS, previously isolated from Gansu province, was cloned and compared with published sequence data from other TGEV strains. Phylogenetic tree analysis based on the amino acid and nucleotide sequences of the S gene showed that the TGEV strains were divided into 3 clusters. TGEV TS showed a close evolutionary relationship to the American Miller cluster but had a 5' non-translated region (NTR) sequence closely related to the American Purdue cluster. Continued culture in different cell types indicated that TGEV TS virulence could be attenuated after fifty passages in Porcine kidney (PK-15) cells, and that the Porcine kidney cell line IB-RS-2 (IBRS) was not suitable for culture of the TGEV strain TS.
Key laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.
Jian-qiang
JQ
Li
Jie
J
Cheng
Xi
X
Lan
Xue-rui
XR
Li
Wei
W
Li
Xiang-ping
XP
Yin
Bao-yu
BY
Li
Bin
B
Yang
Zhi-yong
ZY
Li
Yun
Y
Zhang
Ji-xing
JX
Liu
D020413:3' Untranslated Regions; D000818:Animals; D002460:Cell Line; D016000:Cluster Analysis; D016679:Genome, Viral; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D012692:Serial Passage; D013552:Swine; D005760:Transmissible gastroenteritis virus / Q000235:genetics* / Q000472:pathogenicity; D014774:Virulence
D020413:3' Untranslated Regions; D012367:RNA, Viral