Measurement of bioavailability: measurement of absorption through skin in vitro.
New therapeutic compounds intended for use on the skin or for delivery through application to the skin and agrochemicals, whose use may result in skin exposure, must be tested for bioavailability as the result of absorption. This unit contains a protocol for measuring skin absorption in vitro using the diffusion cell skin absorption method (SAM), which can be used to measure percutaneous absorption after topical application. Usually a radiolabeled compound is used, but if a suitable specific assay is available, nonradioactive compounds may be tested. The procedure is applicable to skin from a variety of species.
Imperial College School of Medicine, London, United Kingdom.
S A
SA
Hotchkiss
D000279:Administration, Cutaneous; D016573:Agrochemicals / Q000493:pharmacokinetics; D000826:Animal Testing Alternatives; D000818:Animals; D001682:Biological Availability; D006801:Humans; D008954:Models, Biological; D004364:Pharmaceutical Preparations / Q000008:administration & dosage / Q000378:metabolism; D012867:Skin / Q000378:metabolism*; D012869:Skin Absorption; D013048:Specimen Handling / Q000379:methods / Q000592:standards; D046509:Tissue Culture Techniques / Q000295:instrumentation / Q000379:methods / Q000592:standards; D014116:Toxicology / Q000295:instrumentation / Q000379:methods* / Q000592:standards
D016573:Agrochemicals; D004364:Pharmaceutical Preparations