In vivo inhibition of NAS preparation on H9N2 subtype AIV.
NAS preparation, a kind of Chinese herbal medicine found by the Yunnan Eco-agricultural Research Institute, has potential antiviral activity. In this paper, the inhibiting effect of NAS preparation on H9N2 subtype Avian influenza virus (AIV) was investigated in vivo. Chickens infected with H9N2 virus were treated with NAS preparation for 4 days. The virus was then detected by hemoagglutination (HA) test and reverse transcription polymerase chain reaction (RT-PCR). The results showed that no H9N2 virus could be detected at the 7th day when the chickens were treated with 0.2 g/kg/d or 0.1 g/kg/d of NAS preparation. However the virus could be detected in other chickens without NAS preparation treatment. This result suggested that NAS preparation may be a potential drug candidate to control infection of H9N2 subtype AIV in chickens.
Shang
Ruo-feng
RF
Key Laboratory of New Animal Medicine Project, Chinese Academy of Agricultural Sciences, Lanzhou, China.
Liang
Jian-ping
JP
Na
Zhong-yuan
ZY
Yang
Hong-jun
HJ
Lu
Yu
Y
Hua
Lan-ying
LY
Guo
Wen-zhu
WZ
Cui
Ying
Y
Wang
Ling
L
D000818:Animals; D000998:Antiviral Agents / Q000008:administration & dosage*; D002645:Chickens; D002988:Cloaca / Q000821:virology; D006386:Hemagglutination Tests; D053127:Influenza A Virus, H9N2 Subtype / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D005585:Influenza in Birds / Q000188:drug therapy* / Q000821:virology; D010614:Pharynx / Q000821:virology; D010936:Plant Extracts / Q000008:administration & dosage*; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors
D000998:Antiviral Agents; D010936:Plant Extracts; D012367:RNA, Viral; C015378:nas