Sexual dimorphism in the brain aromatase expression and activity, and in the central expression of other steroidogenic enzymes during the period of sex differentiation in monosex rainbow trout populations.
Using genetic monosex male and female rainbow trout populations, the potential sex differences in the central expression of estrogen receptors (esr1, esr2a, esr2b), brain aromatase (cyp19a1b) and some other steroidogenic enzymes was studied over the period of sex differentiation (from 35 to 63 dpf: days post-fertilization) using quantitative polymerase chain reaction (q-PCR). In addition, aromatase activity was evaluated during this period. The results indicated that brain aromatase (cyp19a1b) expression and activity showed a clear and significant sexually dimorphic pattern with higher levels in male brain between 35 and 53 dpf before the time of gonad morphological differentiation. At that time the expression of a key enzyme involved in the conversion of cholesterol into steroids, the cyp11a1 (p450scc), as well as the estrogen receptors were also sexually dimorphic. The dimorphism was lost from 56 dpf onwards. Transcription factors such as nr5a1b (sf1) and nr0b1 (dax1), but not foxl2a were also higher in males than in females. These results demonstrate that, before or during the early period of morphological gonad differentiation, the brain exhibits a clear sexual dimorphism with respect to the expression and activity of aromatase as well as of certain enzymes and factors involved in steroid synthesis as p450scc and sf1. The results suggest a higher potentiality to produce estrogens by male brains during sex differentiation time.
Vizziano-Cantonnet
Denise
D
Facultad de Ciencias, Laboratorio de Fisiología de la Reproducción y Ecología de Peces, Iguá 4225, Montevideo 11400, Uruguay. vizziano@gmail.com
Anglade
Isabelle
I
Pellegrini
Elisabeth
E
Gueguen
Marie-Madeleine
MM
Fostier
Alexis
A
Guiguen
Yann
Y
Kah
Olivier
O
D000818:Animals; D001141:Aromatase / Q000235:genetics / Q000378:metabolism*; D001921:Brain / Q000201:enzymology*; D002784:Cholesterol / Q000378:metabolism; D005260:Female; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism*; D008297:Male; D017686:Oncorhynchus mykiss / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D011960:Receptors, Estrogen / Q000235:genetics / Q000378:metabolism; D012727:Sex Characteristics; D012733:Sex Differentiation
D029941:Fish Proteins; D012333:RNA, Messenger; D011960:Receptors, Estrogen; D002784:Cholesterol; D001141:Aromatase