Impaired aldosterone responsiveness in corticosteroid binding globulin deficient mice.
OBJECTIVE
Corticosteroid binding globulin (CBG) is the high affinity plasma carrier protein for cortisol. It keeps the steroids inactive, prevents them from degradation and defines the amount of free hormone acting on target tissues. Previous findings have shown insufficient responsiveness of corticosterone in peripheral tissues in CBG⁻(/)⁻ mice despite elevated free plasma corticosterone. In the large intestine, glucocorticoids synergistically enhance the pro-absorptive effects of aldosterone. We therefore hypothesized that CBG⁻(/)⁻ mice have reduced responsiveness to aldosterone.


METHODS
We used CBG⁻(/)⁻ and CBG(+/+) mice to investigate distal colonic electrogenic Na(+) absorption. An Ussing chamber was used to quantify amiloride-sensitive Na(+) transport in distal colonic mucosa (ΔI(sc) (amil)) as a measure of the physiological effect of aldosterone.


RESULTS
No differences were observed in ΔI(sc) (amil) or aldosterone levels in animals on control diet. When Na(+) restricted, CBG(+/+) mice responded with a marked up-regulation of ΔI(sc) (amil) (25-fold). In CBG⁻(/)⁻ mice this up-regulation was greatly attenuated as seen in a markedly reduced amiloride-sensitive short circuit current (reduced by ∼50%), a reduced ability to lower faecal Na(+) excretion and a significantly attenuated up-regulation of the ENaC channel γ-subunit. Diet-induced increases of total plasma aldosterone were similar in both genotypes, but CBG⁻(/)⁻ mice had an increased free plasma aldosterone fraction.


CONCLUSIONS
This study defines the functional hyporesponsiveness and aldosterone resistance in distal colon of CBG⁻(/)⁻ mice. This resistance occurs despite sufficient free corticosterone plasma level. Thus, steroid actions require an intrinsic but unknown function of CBG, which allows the sufficient supply of the hormone/s to the target tissue.
Department of Physiology and Biophysics, The Water and Salt Research Center, Aarhus University, Denmark.
M V
MV
Sorensen
H A
HA
Praetorius
A
A
Nykjaer
T
T
Willnow
J
J
Leipziger
D000450:Aldosterone / Q000378:metabolism*; D000584:Amiloride / Q000378:metabolism; D000818:Animals; D003345:Corticosterone / Q000378:metabolism; D062686:Epithelial Sodium Channel Blockers; D053503:Epithelial Sodium Channels / Q000378:metabolism; D005243:Feces / Q000737:chemistry; D007408:Intestinal Absorption / Q000502:physiology; D007413:Intestinal Mucosa / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D012964:Sodium / Q000378:metabolism; D012982:Sodium, Dietary / Q000378:metabolism; D014156:Transcortin / Q000235:genetics / Q000378:metabolism*
D062686:Epithelial Sodium Channel Blockers; D053503:Epithelial Sodium Channels; D012982:Sodium, Dietary; D000450:Aldosterone; D000584:Amiloride; D014156:Transcortin; D012964:Sodium; D003345:Corticosterone