Increasing the stability of an enzyme toward hostile organic solvents by directed evolution based on iterative saturation mutagenesis using the B-FIT method.
Mutants of the lipase from Bacillus subtilis, previously engineered for enhanced thermostability using directed evolution based on the B-FIT method, show significantly increased tolerance to hostile organic solvents.
Reetz
Manfred T
MT
Max-Planck-Institut für Kohlenforschung, Kaiser-Wilhelm-Platz 1, 45470 Mülheim an der Ruhr, Germany. reetz@mpi-muelheim.mpg
Soni
Pankaj
P
Fernández
Layla
L
Gumulya
Yosephine
Y
Carballeira
José D
JD
D001412:Bacillus subtilis / Q000201:enzymology; D019020:Directed Molecular Evolution / Q000379:methods*; D004795:Enzyme Stability / Q000187:drug effects / Q000235:genetics; D008049:Lipase / Q000737:chemistry* / Q000235:genetics*; D016296:Mutagenesis; D009930:Organic Chemicals / Q000494:pharmacology*; D012997:Solvents / Q000494:pharmacology*
D009930:Organic Chemicals; D012997:Solvents; D008049:Lipase