Herpes virus reactivation by low-intensity diode and CO₂ lasers.
BACKGROUND
The herpes virus enters into latency after symptomatic or asymptomatic herpetic infection. During latency, the virus has no impact on infected cells. However, internal or external stimuli, including certain lasers, can induce virus reactivation.


OBJECTIVE
The aim was to study the reactivation power of the low-intensity diode and CO(2) lasers on the latent herpes virus.


METHODS
The bovine herpesvirus 1 (BHV-1) was inoculated in either the nasal cavity or the lacrimal film of an animal model. Once the virus entered into latency, the trigeminal ganglia of animals were exposed to either a low-intensity diode or CO(2) laser. The reactivation of the virus was then explored by PCR, RT-PCR, and dot-blot hybridization on nasal or lacrimal swabs. The accuracy, sensitivity, and specificity of the aforementioned techniques were compared.


RESULTS
The low-intensity diode laser reactivated the herpes virus less than the CO(2) laser. The nasally inoculated virus was more liable for reactivation by both lasers. PCR was considered as the standard method for the detection of the reactivated virus.


CONCLUSIONS
Low-intensity diode and CO(2) lasers can induce herpes virus reactivation, with the diode laser less likely to reactivate the virus than the CO(2) laser.
Saleh
Hazem Mohammad
HM
Otolaryngology unit, National Institute of Laser Enhanced Sciences, Cairo University, Egypt. hazemsalehniles@yahoo.com
Shaker
Ahmed Samir
AS
Saafan
Ali Mohamed
AM
Ibrahim
Adel Khalil
AK
D000818:Animals; D004195:Disease Models, Animal; D004587:Electrophoresis, Agar Gel; D006564:Herpesviridae / Q000502:physiology*; D054020:Lasers, Gas / Q000009:adverse effects*; D011817:Rabbits; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017735:Virus Latency