An easy and efficient fluorescent method for detecting aldehydes and its application in biotransformation.
Water-soluble aldehydes (acetaldehyde, propionaldehyde) and non-water-soluble aldehydes (butyraldehyde and phenylacetaldehyde) were easily detected by an efficient fluorescent method with 5-aminofluorescein as probe. Under optimal detection conditions, 5-aminofluorescein could selectively respond to aldehydes with high sensitivity in comparison with other carbonyl compounds like ketones and acids. Thus, the proposed method was used to monitor microbial oxidation and succeeded in trapping transiently-produced aldehydes during biotransformation of primary alcohols by Gluconobacter oxydans.
Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, People's Republic of China.
Yanlong
Y
Xing
Shu
S
Wang
Xiangzhao
X
Mao
Xuebo
X
Zhao
Dongzhi
D
Wei
D000042:Absorption; D000438:Alcohols / Q000378:metabolism; D000447:Aldehydes / Q000032:analysis* / Q000737:chemistry; D001711:Biotransformation; D002138:Calibration; D005452:Fluoresceins / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D020600:Gluconobacter oxydans / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010084:Oxidation-Reduction; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013379:Substrate Specificity
D000438:Alcohols; D000447:Aldehydes; D005452:Fluoresceins; D005456:Fluorescent Dyes; C030864:5-aminofluorescein; C032251:isovalerylaldehyde