Localization of eukaryote-specific ribosomal proteins in a 5.5-Å cryo-EM map of the 80S eukaryotic ribosome.
Protein synthesis in all living organisms occurs on ribonucleoprotein particles, called ribosomes. Despite the universality of this process, eukaryotic ribosomes are significantly larger in size than their bacterial counterparts due in part to the presence of 80 r proteins rather than 54 in bacteria. Using cryoelectron microscopy reconstructions of a translating plant (Triticum aestivum) 80S ribosome at 5.5-Å resolution, together with a 6.1-Å map of a translating Saccharomyces cerevisiae 80S ribosome, we have localized and modeled 74/80 (92.5%) of the ribosomal proteins, encompassing 12 archaeal/eukaryote-specific small subunit proteins as well as the complete complement of the ribosomal proteins of the eukaryotic large subunit. Near-complete atomic models of the 80S ribosome provide insights into the structure, function, and evolution of the eukaryotic translational apparatus.
Armache
Jean-Paul
JP
Gene Center, Department of Biochemistry, Ludwig-Maximilians-Universität München, Feodor-Lynen-Strasse 25, 81377 Munich, Germany.
Jarasch
Alexander
A
Anger
Andreas M
AM
Villa
Elizabeth
E
Becker
Thomas
T
Bhushan
Shashi
S
Jossinet
Fabrice
F
Habeck
Michael
M
Dindar
Gülcin
G
Franckenberg
Sibylle
S
Marquez
Viter
V
Mielke
Thorsten
T
Thomm
Michael
M
Berninghausen
Otto
O
Beatrix
Birgitta
B
Söding
Johannes
J
Westhof
Eric
E
Wilson
Daniel N
DN
Beckmann
Roland
R
D020285:Cryoelectron Microscopy; D005057:Eukaryotic Cells / Q000378:metabolism* / Q000648:ultrastructure*; D019143:Evolution, Molecular; D008958:Models, Molecular; D021381:Protein Transport; D012335:RNA, Ribosomal / Q000737:chemistry / Q000235:genetics / Q000648:ultrastructure; D012269:Ribosomal Proteins / Q000378:metabolism* / Q000648:ultrastructure*; D012270:Ribosomes / Q000378:metabolism / Q000648:ultrastructure*; D012441:Saccharomyces cerevisiae / Q000378:metabolism / Q000648:ultrastructure; D013045:Species Specificity; D014908:Triticum / Q000378:metabolism
D012335:RNA, Ribosomal; D012269:Ribosomal Proteins