Polymorphisms in the 18S rDNA gene of Cystoisospora belli and clinical features of cystoisosporosis in HIV-infected patients.
Intraspecific variability among Cystoisospora belli isolates and its clinical implications in human cystoisosporosis have not been established. In this study, the restriction fragment length polymorphisms in a 1.8-kb amplicon of the small subunit ribosomal DNA (SSU rDNA) of the parasite was investigated in 20 C. belli-positive stool samples obtained from 15 HIV-infected patients. Diarrheic syndrome was observed in all patients with cystoisosporosis and the number of diarrheic episodes per patient during hospitalization ranged from 1 to 26 (mean of 9.64 ± 9.30), with a mean duration of 2 to 12 days (mean of 5.90 ± 3 days). Three restriction profiles (RF) were generated with MboII digestion, which were named RFI, RFII, and RFIII. Two isolates obtained from a patient with extraintestinal cystoisosporosis showed distinct restriction profiles with MboII. This study demonstrates that patients can be infected with different C. belli genotypes, and this information may be useful for identifying new C. belli genotypes infecting humans.
Resende
Deisy V
DV
Disciplina de Parasitologia, Universidade Federal do Triângulo Mineiro, Rua Frei Paulino 30, Abadia, CEP 38025-180 Uberaba, Minas Gerais, Brazil.
Pedrosa
André L
AL
Correia
Dalmo
D
Cabrine-Santos
Marlene
M
Lages-Silva
Eliane
E
Meira
Wendell S F
WS
Oliveira-Silva
Márcia B
MB
D000328:Adult; D003048:Coccidiosis / Q000150:complications* / Q000469:parasitology*; D016054:DNA, Protozoan / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics*; D005243:Feces / Q000469:parasitology; D005260:Female; D020459:Genes, rRNA; D005819:Genetic Markers; D015658:HIV Infections / Q000150:complications*; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D021745:Sarcocystidae / Q000235:genetics* / Q000302:isolation & purification*; D055815:Young Adult
D016054:DNA, Protozoan; D004275:DNA, Ribosomal; D005819:Genetic Markers; D012337:RNA, Ribosomal, 18S