Purification of Rubisco activase from leaves or after expression in Escherichia coli.
Rubisco activase is a molecular chaperone that modulates the activation state of Rubisco by catalyzing the ATP-dependent removal of tightly-bound inhibitory sugar-phosphates from Rubisco's catalytic sites. This chapter reports methods developed for the purification of native and recombinant Rubisco activase from leaves and bacterial cells, respectively.
Barta
Csengele
C
U.S. Department of Agriculture, Arid-Land Agricultural Research Center, Maricopa, AZ, USA.
Carmo-Silva
A Elizabete
AE
Salvucci
Michael E
ME
D000645:Ammonium Sulfate / Q000737:chemistry; D000818:Animals; D017360:Arabidopsis / Q000201:enzymology; D049109:Cell Proliferation; D005591:Chemical Fractionation / Q000379:methods*; D011232:Chemical Precipitation; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004926:Escherichia coli / Q000166:cytology / Q000235:genetics*; D015870:Gene Expression; D018515:Plant Leaves / Q000201:enzymology*; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification*
D010940:Plant Proteins; C054765:rca protein, plant; D000645:Ammonium Sulfate