Age associated oxidative damage in lymphocytes.
Lymphocytes are an important immunological cell and have been played a significant role in acquired immune system; hence, may play in pivotal role in immunosenescence. Oxidative stress has been reported to increase in elderly subjects, possibly arising from an uncontrolled production of free radicals with aging and decreased antioxidant defenses. This study was aimed to evaluate the level of lipid-protein damage and antioxidant status in lymphocytes of healthy individuals to correlate between oxidative damage with the aging process. Twenty healthy individuals of each age group (11-20; 21-30; 31-40; 41-50; and 51-60 years) were selected randomly. Blood samples were drawn by medical practitioner and lymphocytes were isolated from blood samples. Malondialdehyde (MDA), protein carbonyls (PC) level were evaluated to determine the lipid and protein damage in lymphocytes. Superoxide dismutase (SOD), catalase (CAT), glutathione and glutathione dependent enzymes were estimated to evaluate the antioxidant status in the lymphocytes. Increased MDA and PC levels strongly support the increased oxidative damage in elderly subject than young subjects. The results indicated that, balance of oxidant and antioxidant systems in lymphocytes shifts in favor of accelerated oxidative damage during aging. Thus oxidative stress in lymphocytes may particular interest in aging and may play important role in immunosenescence.
Gautam
Nandeslu
N
Immunology and Microbiology Laboratory; Department of Human Physiology with Community Health; Vidyasagar University; Midnapore, West Bengal India.
Das
Subhasis
S
Immunology and Microbiology Laboratory; Department of Human Physiology with Community Health; Vidyasagar University; Midnapore, West Bengal India.
Kar Mahapatra
Santanu
S
Immunology and Microbiology Laboratory; Department of Human Physiology with Community Health; Vidyasagar University; Midnapore, West Bengal India.
Chakraborty
Subhankari Prasad
SP
Immunology and Microbiology Laboratory; Department of Human Physiology with Community Health; Vidyasagar University; Midnapore, West Bengal India.
Kundu
Pratip Kumar
PK
Department of Microbiology; Vidyasagar University; Midnapore, West Bengal India.
Roy
Somenath
S
Immunology and Microbiology Laboratory; Department of Human Physiology with Community Health; Vidyasagar University; Midnapore, West Bengal India.
D000293:Adolescent; D000328:Adult; D000375:Aging; D002648:Child; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000473:pathology*; D008875:Middle Aged; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000502:physiology*; D013482:Superoxide Dismutase / Q000378:metabolism; D055815:Young Adult
D013482:Superoxide Dismutase