Blind frequency-resolved optical-gating pulse characterization for quantitative differential multiphoton microscopy.
We use a unique multifocal multiphoton microscope to directly characterize the pulse in the focal plane of a high-NA objective using second-harmonic generation frequency-resolved optical gating (FROG). Because of the nature of the optical setup, femtosecond laser pulses of orthogonal polarization states are generated in the focal plane, each acquiring a different spectral dispersion. By applying an additional constraint on the phase extraction algorithm, we simultaneously extract both the gate and probe pulses from a single spectrogram with a FROG error of 0.016.
Center for Microintegrated Optics for Advanced Bioimaging and Control, Department of Physics,Colorado School of Mines, 1523 Illinois Street, Golden, Colorado 80401, USA. jjfield@mines.edu
Jeffrey J
JJ
Field
Charles G
CG
Durfee
Jeff A
JA
Squier
D000465:Algorithms; D007089:Image Enhancement / Q000295:instrumentation* / Q000379:methods; D007834:Lasers; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation* / Q000379:methods; D055095:Optics and Photonics; D015203:Reproducibility of Results; D012815:Signal Processing, Computer-Assisted; D013057:Spectrum Analysis / Q000379:methods