Real-time polymerase chain reaction of "red complex" (Porphyromonas gingivalis, Tannerella forsythia, and Treponema denticola) in periradicular abscesses.
OBJECTIVE
The red complex bacteria (Porphyromonas gingivalis, Tannerella forsythia, and Treponema denticola) have been implicated, either singly or in combination, in the development of various forms of periodontal diseases. The aim of this study was to investigate the presence of "red complex" in acute periradicular abscesses by real-time polymerase chain reaction (PCR) method.


METHODS
Microbial samples were collected by aspiration from 32 cases diagnosed as acute periradicular abscess. DNA was extracted from the samples by using a QIAamp DNA mini-kit and analyzed with real-time PCR.


RESULTS
At least 1 member of the red complex was found in 84% of the cases. In general T. denticola, P. gingivalis, and T. forsythia were detected in 65.6%, 43.7%, and 40.6% of the cases, respectively. Red complex was detected in 15.6% of samples taken from acute periradicular abscesses.


CONCLUSIONS
Our findings suggest that "red complex" can participate in the pathogenesis of acute periradicular abscesses.
Ozbek
Selcuk M
SM
Department of Endodontics, Dental School, Ataturk University, Erzurum, Turkey. selcuk233@yahoo.com
Ozbek
Ahmet
A
D000293:Adolescent; D000328:Adult; D016301:Alveolar Bone Loss / Q000382:microbiology; D016866:Bacteroidaceae Infections / Q000382:microbiology; D001439:Bacteroides / Q000302:isolation & purification*; D001442:Bacteroides Infections / Q000382:microbiology; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis; D003731:Dental Caries / Q000382:microbiology; D003790:Dental Pulp Necrosis / Q000382:microbiology; D006801:Humans; D008875:Middle Aged; D010482:Periapical Abscess / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D016966:Porphyromonas gingivalis / Q000302:isolation & purification*; D048373:Treponema denticola / Q000302:isolation & purification*; D014211:Treponemal Infections / Q000382:microbiology; D055815:Young Adult
D017931:DNA Primers; D004269:DNA, Bacterial