Detection of APP intracellular domain in brain tissue.
The cleavage of amyloid precursor protein (APP) by γ-secretase produces Aβ peptides, which are prominent features in Alzheimer's disease and have been extensively studied. By contrast, APP intracellular domain (AICD), also a product of this cleavage event, has received little or no investigative attention. A major reason for this is that AICD is generally not detected in tissue lysates and, therefore, is neglected as a non-relevant product of APP metabolism. However, recent studies have shown that AICD regulates a number of important cellular events. Furthermore, we found that contrary to previous assertions, AICD can be detected in brain lysates using Western blotting if an antigen retrieval protocol is employed. Here we describe the protocol for AICD detection and note the biological relevance of AICD in physiological and pathological conditions.
Department of Neurosciences, Lerner Research Institute, Cleveland Clinic, Cleveland, OH, USA.
Sanjay W
SW
Pimplikar
Anupama
A
Suryanarayana
D016564:Amyloid beta-Protein Precursor / Q000378:metabolism*; D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D051379:Mice; D008822:Mice, Transgenic; D017434:Protein Structure, Tertiary
D016564:Amyloid beta-Protein Precursor