Response surface optimization of oxalic acid pretreatment of yellow poplar (Liriodendron tulipifera) for production of glucose and xylose monosaccarides.
The primary goal of this study was to determine the optimal condition to obtain fermentable monosaccharides (xylose and glucose) from hydrolysates of yellow poplar (Liriodendron tulipifera) by oxalic acid pretreatment as a potential bio-ethanol source. Based on 2(3) factorial design, fifteen operations were performed by varying on acid loading, reaction time and temperature, and the components of the solid and liquid fractions were analyzed. The sugar concentration (g/L) in hydrolysates and xylose solubilization (%) were applied to response surface methodology. The optimal condition for producing sugars was 151 °C, 0.042 g/g (weight of oxalic acid/dry matter), 13 min with predicted yield of 37.4 g/L, and for the xylose solubilization was 158 °C, 0.037 g/g, 13 min yielding 72.0% of the predicted value. Severe conditions generated inhibitors. By measuring the concentrations, the possibility utilizing hydrolysates for fermentation were estimated.
Kim
Hye-Yun
HY
Department of Forest Sciences, College of Agriculture and Life Sciences, Seoul National University, 599 Gwanak-ro, Gwanak-gu, Seoul 151-921, South Korea.
Lee
Jae-Won
JW
Jeffries
Thomas W
TW
Choi
In-Gyu
IG
D000704:Analysis of Variance; D001709:Biotechnology / Q000379:methods*; D005285:Fermentation / Q000187:drug effects; D005947:Glucose / Q000096:biosynthesis*; D006868:Hydrolysis / Q000187:drug effects; D031567:Liriodendron / Q000187:drug effects* / Q000378:metabolism*; D019815:Oxalic Acid / Q000494:pharmacology*; D012995:Solubility / Q000187:drug effects; D013696:Temperature; D013997:Time Factors; D014994:Xylose / Q000096:biosynthesis*
D019815:Oxalic Acid; D014994:Xylose; D005947:Glucose