Organotypic 3D cell culture models: using the rotating wall vessel to study host-pathogen interactions.
Appropriately simulating the three-dimensional (3D) environment in which tissues normally develop and function is crucial for engineering in vitro models that can be used for the meaningful dissection of host-pathogen interactions. This Review highlights how the rotating wall vessel bioreactor has been used to establish 3D hierarchical models that range in complexity from a single cell type to multicellular co-culture models that recapitulate the 3D architecture of tissues observed in vivo. The application of these models to the study of infectious diseases is discussed.
Center for Infectious Diseases and Vaccinology, Arizona State University, Tempe, AZ 85287, USA.
Jennifer
J
Barrila
Andrea L
AL
Radtke
Aurélie
A
Crabbé
Shameema F
SF
Sarker
Melissa M
MM
Herbst-Kralovetz
C Mark
CM
Ott
Cheryl A
CA
Nickerson
D000818:Animals; D001698:Biomedical Engineering; D019149:Bioreactors; D002478:Cells, Cultured; D003141:Communicable Diseases / Q000209:etiology; D054884:Host-Pathogen Interactions / Q000502:physiology*; D006801:Humans; D066298:In Vitro Techniques; D008954:Models, Biological; D012399:Rotation