Naphthylchalcones induce apoptosis and caspase activation in a leukemia cell line: The relationship between mitochondrial damage, oxidative stress, and cell death.
In this study, we investigated the effects of 24 chalcone derivatives from 2-naphthylacetophenone toward a lymphoblastic leukemia cell line (L1210). Three compounds, called R7, R13, and R15, presented concentration- and time-dependent cytotoxicity and induced cellular death by apoptosis via mitochondrial injury and oxidative stress. The effects of these compounds appear to occur through different mechanisms because R13 and R7 induced a greater disturbance of mitochondrial potential, and all compounds induced disturbances of cellular ATP content and increased caspase-3 activity before cellular death. These compounds also interfered with antioxidant enzymes activities and GSH content through different mechanisms.
Departamento de Ciências Farmacêuticas, Universidade Federal de Santa Catarina, 88040-900 Florianópolis, SC, Brazil.
Evelyn
E
Winter
Louise Domeneghini
LD
Chiaradia
Clarissa A S
CA
de Cordova
Ricardo José
RJ
Nunes
Rosendo Augusto
RA
Yunes
Tânia Beatriz
TB
Creczynski-Pasa
D000970:Antineoplastic Agents / Q000737:chemistry* / Q000627:therapeutic use / Q000633:toxicity; D017209:Apoptosis; D053148:Caspase 3 / Q000378:metabolism*; D045744:Cell Line, Tumor; D002599:Chalcone / Q000031:analogs & derivatives* / Q000627:therapeutic use / Q000633:toxicity; D047188:Chalcones / Q000737:chemistry* / Q000627:therapeutic use / Q000633:toxicity; D006801:Humans; D007938:Leukemia / Q000188:drug therapy*; D008928:Mitochondria / Q000187:drug effects*; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000378:metabolism
D000970:Antineoplastic Agents; D047188:Chalcones; D017382:Reactive Oxygen Species; D002599:Chalcone; D053148:Caspase 3