Dark-field optical coherence microscopy.
Dark-field illumination is known to enhance scattering contrast in optical microscopy. We combined this concept with Fourier domain optical coherence microscopy (OCM). The detection and illumination paths are decoupled, and only the scattered light originating from the sample generates the tomogram signal, whereas any specular reflection is highly suppressed. We analyze and discuss this dark-field OCM concept and present its superior imaging quality on live cell samples.
Laboratoire d’Optique Biomédicale, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland. martin.villiger@epfl.ch
Martin
M
Villiger
Christophe
C
Pache
Theo
T
Lasser
D000818:Animals; D007089:Image Enhancement / Q000379:methods; D007090:Image Interpretation, Computer-Assisted / Q000379:methods; D008027:Light; D051379:Mice; D008853:Microscopy / Q000379:methods*; D041681:NIH 3T3 Cells; D041623:Tomography, Optical Coherence / Q000379:methods*