A simple method to record parietal cells in the fundic mucosa in baboons.
BACKGROUND
Gastric parietal cells in a baboon were recently found to be auto-fluorescent.


OBJECTIVE
To study gastric sections with a fluorescent microscope in a cohort of baboons.


METHODS
Gastric sections from 38 baboons were stained with hematoxylin-eosin (H&E) and examined in a fluorescence microscope (FLM). The thickness of the parietal cell population was assessed at x 10 magnification.


RESULTS
H&E stained all mucosal cells: fovelar, parietal and chief cells. When the same sections were analyzed with an FLM, only parietal cells were auto-fluorescent, whereas fovelar and chief cells remained non-fluorescent. Parietal cells formed a distinct, continuous auto-fluorescent band. The ratio of the auto-fluorescent parietal cell band/total mucosa ranged between 0.20 and 0.30.


CONCLUSIONS
Gastric parietal cells became auto-fluorescent when H&E-stained sections from baboon stomachs were observed with an FLM. Eosin was the stain responsible for this optical phenomenon.
Gastrointestinal and Liver Pathology Research Laboratory, Department of Pathology, Karolinska Institute and University Hospital, 17176 Stockholm, Sweden. Carlos.Rubio@ki.se
Carlos A
CA
Rubio
Michael
M
Owston
Abiel
A
Orrego
Edward J
EJ
Dick
D000818:Animals; D019872:Chief Cells, Gastric / Q000166:cytology; D004396:Coloring Agents; D004801:Eosine Yellowish-(YS); D005456:Fluorescent Dyes; D005748:Gastric Fundus / Q000166:cytology*; D006416:Hematoxylin; D008856:Microscopy, Fluorescence / Q000379:methods*; D010215:Papio; D010295:Parietal Cells, Gastric / Q000166:cytology*
D004396:Coloring Agents; D005456:Fluorescent Dyes; D004801:Eosine Yellowish-(YS); D006416:Hematoxylin