Chitotriosidase activity predicts endothelial dysfunction in type-2 diabetes mellitus.
The enzyme chitotriosidase (ChT) is secreted by activated macrophages and play active role in human immune response. ChT activity is increased in atherosclerosis in association to the extent of the disease. We investigated the relevance of ChT to endothelial functions and insulin resistance in patients with T2DM. Forty newly diagnosed and untreated patients with T2DM (male 17; age 47.0 ± 6.2 years) and 50 healthy volunteers (male 21; age 50.2 ± 8.8 years) were enrolled. Plasma asymmetric dimethyl arginine (ADMA) levels were determined by ELISA. ChT activity was measured by the fluorescence method. Insulin resistance was calculated by the HOMA-IR formula. The patients had higher systolic blood pressures, HOMA-IR, ADMA levels, and ChT activities (P < 0.001 for all) and lower HDL cholesterol levels (P = 0.03) than the control group. The ChT activities of the total group were significantly correlated to the age (r = 0.031, p = 0.003), ADMA (r = 0.22, p = 0.04), and plasma glucose levels (r = 0.27, p = 0.01). ChT was the independent determinant of the plasma ADMA levels (r = 0.26, p = 0.02). The results of this study show that serum ChT activity is increased in patients with newly diagnosed, untreated, and uncomplicated patients with T2DM. The results also imply that increased ChT activity may be a predictor of endothelial dysfunction.
Departments of Endocrinology, Gulhane School of Medicine, 06018 Etlik, Ankara, Turkey. alpersonmez@yahoo.com
Alper
A
Sonmez
Cem
C
Haymana
Serkan
S
Tapan
Umut
U
Safer
Gurkan
G
Celebi
Ozlem
O
Ozturk
Halil
H
Genc
Teoman
T
Dogru
Ilker
I
Tasci
Gokhan
G
Erdem
Abdullah
A
Taslipinar
Aydogan
A
Aydogdu
Mahmut I
MI
Yilmaz
Ismail
I
Kurt
Mustafa
M
Kutlu
D000328:Adult; D001120:Arginine / Q000031:analogs & derivatives / Q000097:blood; D003924:Diabetes Mellitus, Type 2 / Q000201:enzymology* / Q000503:physiopathology; D004727:Endothelium / Q000503:physiopathology; D005260:Female; D006596:Hexosaminidases / Q000097:blood*; D006801:Humans; D008297:Male; D008875:Middle Aged; D055815:Young Adult
C018524:N,N-dimethylarginine; D001120:Arginine; D006596:Hexosaminidases; C086400:chitotriosidase