Glyceraldehyde-3-phosphate dehydrogenase of the entomopathogenic fungus Metarhizium anisopliae: cell-surface localization and role in host adhesion.
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a classic glycolytic enzyme that plays important roles in various cellular processes. Here, we report the sequence and transcriptional analyses of a regulated gene (gpdh1) encoding GAPDH in the entomopathogenic fungus Metarhizium anisopliae, a well-characterized biocontrol agent of a wide range of arthropod pests. Transcript and protein analyses of the gpdh1 showed a carbohydrate-dependent expression pattern in response to different carbon sources. A demonstration that GAPDH is localized at the cell surface is presented, and assays with insect wings show that this protein has adhesion-like activity. These results imply that GAPDH adhesion to the wing surface is specific and may play a role in the binding of conidia to a host. Our observations indicate new roles for GAPDH both physiologically and during the entomopathogen-host interaction.
Centro de Biotecnologia da Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
Leonardo
L
Broetto
Walter O Beys
WO
Da Silva
Alexandre Melo
AM
Bailão
Célia
C
De Almeida Soares
Marilene Henning
MH
Vainstein
Augusto
A
Schrank
D000818:Animals; D002241:Carbohydrates / Q000494:pharmacology; D002448:Cell Adhesion; D002462:Cell Membrane / Q000201:enzymology / Q000502:physiology; D015966:Gene Expression Regulation, Fungal; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000737:chemistry / Q000235:genetics* / Q000276:immunology / Q000378:metabolism*; D006019:Glycolysis; D020062:Heteroptera / Q000382:microbiology* / Q000502:physiology; D054884:Host-Pathogen Interactions; D052981:Metarhizium / Q000201:enzymology* / Q000378:metabolism / Q000502:physiology*; D011994:Recombinant Proteins / Q000378:metabolism; D012988:Soil Microbiology; D013172:Spores, Fungal / Q000235:genetics / Q000502:physiology; D014158:Transcription, Genetic; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism; D014921:Wings, Animal / Q000382:microbiology
D002241:Carbohydrates; D011994:Recombinant Proteins; D037521:Virulence Factors; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases