Organotypic 3D cell culture models: using the rotating wall vessel to study host-pathogen interactions.
Appropriately simulating the three-dimensional (3D) environment in which tissues normally develop and function is crucial for engineering in vitro models that can be used for the meaningful dissection of host-pathogen interactions. This Review highlights how the rotating wall vessel bioreactor has been used to establish 3D hierarchical models that range in complexity from a single cell type to multicellular co-culture models that recapitulate the 3D architecture of tissues observed in vivo. The application of these models to the study of infectious diseases is discussed.
Barrila
Jennifer
J
Center for Infectious Diseases and Vaccinology, Arizona State University, Tempe, AZ 85287, USA.
Radtke
Andrea L
AL
Crabbé
Aurélie
A
Sarker
Shameema F
SF
Herbst-Kralovetz
Melissa M
MM
Ott
C Mark
CM
Nickerson
Cheryl A
CA
D000818:Animals; D001698:Biomedical Engineering; D019149:Bioreactors; D002478:Cells, Cultured; D003141:Communicable Diseases / Q000209:etiology; D054884:Host-Pathogen Interactions / Q000502:physiology*; D006801:Humans; D066298:In Vitro Techniques; D008954:Models, Biological; D012399:Rotation