Chemical analysis of an immunoenhancing water-soluble polysaccharide of an edible mushroom, Pleurotus florida blue variant.
An immunoenhancing polysaccharide isolated from the aqueous extract of the fruit bodies of the mushroom, Pleurotus florida blue variant, was found to consist of d-glucose and d-galactose in a molar ratio of nearly 5:1. On the basis of sugar analysis, methylation analysis, periodate oxidation, Smith degradation, and NMR studies ((1)H, (13)C, DEPT-135, DQF-COSY, TOCSY, NOESY, ROESY, HMQC, and HMBC), the structure of the repeating unit of the polysaccharide was established as: →6)-β-D-Glcp-(1→3)-α-D-glcp-(1→[6)-β-D-Glcp-(1]3→ α-D=Glcp. The molecule activated macrophages, splenocytes, and thymocytes.
Dey
Biswajit
B
Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721 102, West Bengal, India.
Bhunia
Sanjoy K
SK
Maity
Kankan K
KK
Patra
Sukesh
S
Mandal
Soumitra
S
Maiti
Swatilekha
S
Maiti
Tapas K
TK
Sikdar
Samir R
SR
Islam
Syed S
SS
D000276:Adjuvants, Immunologic / Q000737:chemistry / Q000494:pharmacology; D000818:Animals; D002240:Carbohydrate Sequence; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D008264:Macrophages / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008969:Molecular Sequence Data; D009589:Nitrogen Oxides / Q000378:metabolism; D020076:Pleurotus / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology*; D012995:Solubility; D014867:Water / Q000737:chemistry*
D000276:Adjuvants, Immunologic; D009589:Nitrogen Oxides; D011134:Polysaccharides; D014867:Water