Determination of the antiretroviral drug nevirapine in diluted alkaline electrolyte by adsorptive stripping voltammetry at the mercury film electrode.
This paper describes a stripping method for the determination of nevirapine at the submicromolar concentration levels. The method is based on controlled adsorptive accumulation of nevirapine at thin-film mercury electrode, followed by a linear cyclic scan voltammetry measurement of the surface species. Optimal experimental conditions include a 2.0 x 10(-3) mol L(-1) NaOH solution (supporting electrolyte), an accumulation potential of -0.20 V, and a scan rate of 100 mV s(-1). The response of nevirapine is linear over the concentration range 0.01-0.14 ppm. For an accumulation time of 6 minutes, the detection limit was found to be 0.87 ppb (3.0 x 10(-9) mol L(-1)). More convenient methods to measure the nevirapine in presence of the efavirenz, acyclovir, didanosine, indinavir, nelfinavir, saquinavir, lamivudine, zidovudine and metals ions were also investigated. The utility of this method is demonstrated by the presence of nevirapine together with ATP or DNA.
Department of Chemistry, Pontifícia Universidade Católica do Rio de Janeiro, Rio de Janeiro, RJ, 22451-900, Brazil.
Arnaldo Aguiar
AA
Castro
Ricardo Queiroz
RQ
Aucélio
Nicolás Adrián
NA
Rey
Eliane Monsores
EM
Miguel
Percio Augusto Mardini
PA
Farias
D000327:Adsorption; D000468:Alkalies / Q000737:chemistry*; D004566:Electrodes; D004573:Electrolytes / Q000737:chemistry*; D008628:Mercury / Q000737:chemistry*; D019829:Nevirapine / Q000032:analysis*; D018894:Reverse Transcriptase Inhibitors / Q000032:analysis*
D000468:Alkalies; D004573:Electrolytes; D018894:Reverse Transcriptase Inhibitors; D019829:Nevirapine; D008628:Mercury