HPV-58 molecular variants exhibit different transcriptional activity.
Early promoter activity of HPV-58 molecular variants isolated from high-grade cervical lesions in Brazil was compared. Luciferase reporter assays were conducted in C33 cells transfected with the complete long control region of 3 molecular variants of HPV-58 as well as HPV-58, -18 or -16 prototypes. The HPV-58 prototype and Bsb-329 and Bsb-367 variants showed a promoter activity similar to that of HPV-16, but lower than that of Bsb-295 and HPV-18. The introduction of the Bsb-295 7788 mutation into the HPV-58 prototype resulted in the enhancement of transcription closer to Bsb-295 and HPV-18. These results could impact the expression of E6 and E7 viral oncogenes.
Department of Cellular Biology, Institute of Biology, University of Brasilia, ICC Sul, Brasília, Brazil.
Tainá
T
Raiol
Regina Maria Santos
RM
de Amorim
Pedro
P
Galante
Cláudia Renata Fernandes
CR
Martins
Luisa Lina
LL
Villa
Laura
L
Sichero
D019677:Artificial Gene Fusion; D001938:Brazil; D002460:Cell Line; D002584:Cervix Uteri / Q000473:pathology / Q000821:virology; D005260:Female; D017930:Genes, Reporter; D005838:Genotype; D006801:Humans; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D058889:Molecular Typing; D027383:Papillomaviridae / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D030361:Papillomavirus Infections / Q000821:virology; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic
D008156:Luciferases