Application of serum protein fingerprint in diagnosis of coronary artery disease.
OBJECTIVE
The object of this study is to establish serum protein fingerprint models for early diagnosis and evaluation of luminal stenosis severity of CAD.


METHODS
A total of 189 samples were analyzed by surface-enhanced laser desorption/ionization time of flight mass spectrometry (SELDI-TOF MS) coupled with CM10 chips and bioinformatics tools.


RESULTS
Ten top-ranked proteins were selected as the potential fingerprints of pattern 1, which distinguishes patients with significant CAD (stenosis≥50%) from control individuals. Blind test was analyzed on the second day with a specificity of 79.3% and sensitivity of 78.3%. Pattern 2 distinguishes significant CAD from minimal CAD with luminal stenosis between 25% and 50%. The specificity and sensitivity of blind test for pattern 2 are 77.3% and 73.9%, respectively.


CONCLUSIONS
The SELDI-TOF MS technique combined with bioinformatics approaches may be a novel noninvasive method which can not only facilitate the discovery of diagnostic models for CAD and its severity, but also provide a useful tool for molecular diagnosis.
Wang
Yaping
Y
Cardiovascular Center, Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang Province, PR China.
Hu
Xinyang
X
Wang
Jian-An
JA
Xiang
Meixiang
M
Yu
Jiekai
J
Liu
Fangfei
F
Feng
Yan
Y
D001798:Blood Proteins; D003324:Coronary Artery Disease; D006801:Humans; D010449:Peptide Mapping; D040081:Protein Array Analysis; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D001798:Blood Proteins