Preparation of highly fluorescent magnetic nanoparticles for analytes-enrichment and subsequent biodetection.
Bifunctional nanoparticles with highly fluorescence and decent magnetic properties have been widely used in biomedical application. In this study, highly fluorescent magnetic nanoparticles (FMNPs) with uniform size of ca. 40 nm are prepared by encapsulation of both magnetic nanoparticles (MNPs) and shell/core quantum dots (QDs) with well-designed shell structure/compositions into silica matrix via a one-pot reverse microemulsion approach. The spectral analysis shows that the FMNPs hold high fluorescent quantum yield (QY). The QYs and saturation magnetization of the FMNPs can be regulated by varying the ratio of the encapsulated QDs to MNPs. Moreover, the surface of the FMNPs can be modified to offer chemical groups for antibody conjugation for following use in target-enrichment and subsequent fluorescent detection. The in vitro immunofluorescence assay and flow cytometric analysis indicate that the bifunctional FMNPs-antibody bioconjugates are capable of target-enrichment, magnetic separation and can also be used as alternative fluorescent probes on flow cytometry for biodetection.
Zhang
Bingbo
B
The Institute for Advanced Materials & Nano Biomedicine, Tongji University, Shanghai 200092, China. bingbozhang@tongji.edu.cn
Chen
Bingdi
B
Wang
Yilong
Y
Guo
Fangfang
F
Li
Zhuoquan
Z
Shi
Donglu
D
D000818:Animals; D004655:Emulsions / Q000737:chemistry; D005434:Flow Cytometry / Q000379:methods; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D008280:Magnetics; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology / Q000379:methods*; D010316:Particle Size; D045663:Quantum Dots; D012710:Serum Albumin, Bovine / Q000032:analysis; D012822:Silicon Dioxide / Q000737:chemistry
D004655:Emulsions; D005456:Fluorescent Dyes; D007074:Immunoglobulin G; D012710:Serum Albumin, Bovine; D012822:Silicon Dioxide