Molecular adaptation of an H7N3 wild duck influenza virus following experimental multiple passages in quail and turkey.
To investigate the molecular adaptation of influenza viruses during natural interspecies transmission, we performed a phenotypic and genotypic analysis of a low-pathogenic duck H7N3 influenza virus after experimental passages in turkey and quail. Results obtained showed differences in the HA receptor-binding and in NA enzyme activities in viruses recovered after passages in quail, compared to those obtained from passages in turkey. Sequencing of the HA, NA and genes of internal proteins of the viruses obtained from quail and turkey, identified several amino acid substitutions in comparison with the progenitor virus. Of note, in the quail-adapted viruses the emergence of a 23-amino acid deletion in the stalk of the NA and the introduction of a glycosylation site in the HA were a reminiscence of changes typically observed in nature confirming a potential role of the quail in the adaptation of wild birds viruses to domestic poultry.
Giannecchini
Simone
S
Virology Unit, Department of Public Health, University of Florence, I-50134 Florence, Italy. simone.giannecchini@unifi.it
Clausi
Valeria
V
Di Trani
Livia
L
Falcone
Emiliana
E
Terregino
Calogero
C
Toffan
Anna
A
Cilloni
Filippo
F
Matrosovich
Mikhail
M
Gambaryan
Alexandra S
AS
Bovin
Nicolai V
NV
Delogu
Mauro
M
Capua
Ilaria
I
Donatelli
Isabella
I
Azzi
Alberta
A
D000220:Adaptation, Biological / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D002240:Carbohydrate Sequence; D003370:Coturnix; D004372:Ducks; D005814:Genes, Viral; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000737:chemistry / Q000235:genetics / Q000502:physiology; D057920:Influenza A Virus, H7N3 Subtype / Q000235:genetics / Q000472:pathogenicity* / Q000502:physiology*; D005585:Influenza in Birds / Q000821:virology*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009154:Mutation; D009439:Neuraminidase / Q000235:genetics / Q000502:physiology; D009844:Oligosaccharides / Q000737:chemistry / Q000502:physiology; D011991:Receptors, Virus / Q000502:physiology; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D014422:Turkeys; D014774:Virulence / Q000235:genetics / Q000502:physiology
D019267:Hemagglutinin Glycoproteins, Influenza Virus; D009844:Oligosaccharides; D011991:Receptors, Virus; D009439:Neuraminidase