A comparison of the biological characteristics of EV71 C4 subtypes from different epidemic strains.
The comparative analysis of the biological characterization and the genetic background study of EV71 circulating strains is commonly recognized as basic work necessary for development of an effective EV71 vaccine. In this study, we sequenced five EV71 circulating strains, isolated from Fuyang, Hefei, Kunming and Shenzhen city of China and named them FY-23, FY-22, H44, K9 and S1 respectively. The sequence alignment demonstrated their genotypes be C4. The genetic distance of the VP1 gene from these isolates suggested that they were highly co-related with genetic identity similar to other previously reported EV71 strains in China. Additionally, these strains were identified to display some obvious proliferation dynamics and plaque morphology when propagated in Vero cells. However, a distinctive difference in pathogenic ability in neonatal mice was found. Some differences in cross neutralization test & immunogenic analysis were also found. All these results are related to the biological characterization of circulating EV71 strains in China and aid in the development of an EV71 vaccine in the future.
Institute of Medical Biology, Chinese Academy of Medical Sciences, Peking Union Medical College, Kunming 650118, China.
Li-chun
LC
Wang
Song-qing
SQ
Tang
Yan-mei
YM
Li
Hong-lin
HL
Zhao
Cheng-hong
CH
Dong
Ping-fang
PF
Cui
Shao-hui
SH
Ma
Yun
Y
Liao
Long-ding
LD
Liu
Qi-han
QH
Li
D000818:Animals; D000831:Animals, Newborn; D057134:Antibodies, Neutralizing / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood; D002675:Child, Preschool; D002681:China; D002522:Chlorocebus aethiops; D016000:Cluster Analysis; D004195:Disease Models, Animal; D029821:Enterovirus A, Human / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification* / Q000472:pathogenicity; D004769:Enterovirus Infections / Q000453:epidemiology* / Q000821:virology*; D005838:Genotype; D006801:Humans; D007223:Infant; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D016019:Survival Analysis; D014709:Vero Cells; D010948:Viral Plaque Assay; D015678:Viral Structural Proteins / Q000235:genetics; D014774:Virulence; D014779:Virus Replication
D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D012367:RNA, Viral; D015678:Viral Structural Proteins