Chiral separation of sertraline with microemulsion electrokinetic chromatography on a polymer/β-cyclodextrin assembling molecular film modified capillary.
A capillary modified by assembling a molecular film was presented for the chiral separation of sertraline by microemulsion electrokinetic chromatography. The assembling molecular film was constructed with poly(diallyldimethylammonium-chloride) and β-cyclodextrin via inclusion complexation. The separation efficiency of cis-trans isomers and enantiomers of sertraline was improved with a running microemulsion that contained the acetonitrile, sodium dodecyl sulfate, n-butanol and n-hexane buffered with sodium tetraborate. The baseline separation of four sertraline cis-trans isomers and enantiomers was achieved under the optimum conditions. The detection limit for isomers and enantiomers of sertraline (1S,4S, 1R,4R, 1S,4R, 1R,4S) was 0.15, 0.15, 0.30, 0.30 mg/L, respectively. The mechanism of chiral separation was studied and it could be applied for the determination of commercial Zoloft tablet samples satisfactorily.
Cheng
Hongying
H
Institute of Analytical Chemistry, Department of Chemistry, Dushu Lake Campus, Suzhou University, Suzhou 215123, P. R. China.
He
Baoku
B
Zhang
Qianli
Q
Tu
Yifeng
Y
D000928:Antidepressive Agents / Q000032:analysis* / Q000737:chemistry; D002021:Buffers; D053801:Capillary Electrochromatography; D002845:Chromatography; D004586:Electrophoresis; D006863:Hydrogen-Ion Concentration; D007536:Isomerism; D011108:Polymers; D020280:Sertraline / Q000032:analysis* / Q000737:chemistry; D013237:Stereoisomerism; D047392:beta-Cyclodextrins / Q000378:metabolism
D000928:Antidepressive Agents; D002021:Buffers; D011108:Polymers; D047392:beta-Cyclodextrins; C031215:betadex; D020280:Sertraline