Evaluation of tumorigenic risk of tissue-engineered oral mucosal epithelial cells by using combinational examinations.
Recently, oral mucosal epithelial cells were proposed as a cell source of the autologous cell transplant therapy for corneal trauma or disease. The question addressed is to know if the biological conditions of grafting could induce certain cellular, molecular, and genetic alterations that might increase the risk of mutations and possibly of cellular transformation. Recent progress in cancer research enables us to depict the generation mechanisms and basic characteristics of human cancer cells from molecular, cytological, and biological aspects. The aim of this study is to evaluate the risk of tumorigenicity of the oral mucosal epithelial culture process in order to mitigate that risk, if any, before clinical application. Oral mucosal epithelial cells from three different human donors were investigated by combinational examinations to detect possible tumorigenic transformation. We investigated (i) clonogenic and karyology types, (ii) the validation of proliferation rate, (iii) the epithelial-mesenchymal transition, (iv) anchorage-independent growth potential, and (v) tumorigenicity on nude mice. Results show that the culture process used in this study presents no risk of tumorigenicity.
Thépot
A
A
Banque de Tissus et Cellules, Hôpital E. Herriot, Lyon Cedex 03, France.
Morel
A P
AP
Justin
V
V
Desanlis
A
A
Thivillier
L
L
Hoffman
E
E
Till
M
M
Accardi
R
R
Tommasino
M
M
Breton
P
P
Hainaut
P
P
Damour
O
O
D000328:Adult; D000818:Animals; D015820:Cadherins / Q000378:metabolism; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic; D003316:Corneal Diseases / Q000628:therapy; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism; D058750:Epithelial-Mesenchymal Transition; D006801:Humans; D007621:Karyotyping; D051379:Mice; D008819:Mice, Nude; D009061:Mouth Mucosa / Q000166:cytology*; D012306:Risk; D023822:Tissue Engineering; D014410:Tumor Stem Cell Assay; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D014746:Vimentin / Q000378:metabolism
D015820:Cadherins; D016159:Tumor Suppressor Protein p53; D014746:Vimentin