A simple method to record parietal cells in the fundic mucosa in baboons.
BACKGROUND
Gastric parietal cells in a baboon were recently found to be auto-fluorescent.


OBJECTIVE
To study gastric sections with a fluorescent microscope in a cohort of baboons.


METHODS
Gastric sections from 38 baboons were stained with hematoxylin-eosin (H&E) and examined in a fluorescence microscope (FLM). The thickness of the parietal cell population was assessed at x 10 magnification.


RESULTS
H&E stained all mucosal cells: fovelar, parietal and chief cells. When the same sections were analyzed with an FLM, only parietal cells were auto-fluorescent, whereas fovelar and chief cells remained non-fluorescent. Parietal cells formed a distinct, continuous auto-fluorescent band. The ratio of the auto-fluorescent parietal cell band/total mucosa ranged between 0.20 and 0.30.


CONCLUSIONS
Gastric parietal cells became auto-fluorescent when H&E-stained sections from baboon stomachs were observed with an FLM. Eosin was the stain responsible for this optical phenomenon.
Rubio
Carlos A
CA
Gastrointestinal and Liver Pathology Research Laboratory, Department of Pathology, Karolinska Institute and University Hospital, 17176 Stockholm, Sweden. Carlos.Rubio@ki.se
Owston
Michael
M
Orrego
Abiel
A
Dick
Edward J
EJ
D000818:Animals; D019872:Chief Cells, Gastric / Q000166:cytology; D004396:Coloring Agents; D004801:Eosine Yellowish-(YS); D005456:Fluorescent Dyes; D005748:Gastric Fundus / Q000166:cytology*; D006416:Hematoxylin; D008856:Microscopy, Fluorescence / Q000379:methods*; D010215:Papio; D010295:Parietal Cells, Gastric / Q000166:cytology*
D004396:Coloring Agents; D005456:Fluorescent Dyes; D004801:Eosine Yellowish-(YS); D006416:Hematoxylin