Influence of blood contamination on bond strength of a self-etching adhesive to dental tissues.
OBJECTIVE
The aim of this study was to detect the influence of (1) storage period of heparinized blood, (2) type of blood and presence of contaminant, (3) application mode of cleansing agents, and (4) efficacy of cleansing agents on contaminated enamel and dentin during the adhesion process of a one-step adhesive system.


METHODS
One hundred four human molars were sectioned into halves along the long axis for enamel and dentin tests. Heparinized and fresh blood were obtained from the same donor, applied and dried to maintain a layer of dry blood on the top of samples. The cleansing agents used were hydrogen peroxide, anionic detergent, and antiseptic solution. A one-step adhesive system (Clearfil S3 Bond) was applied on the dental surface, and composite resin cylinders were built up using Tygon tubing molds. After 24 h, the μSBS test (1 mm/min) and fracture analysis were performed.


RESULTS
There was no statistically significant difference in bond strength values regarding the storage period of heparinized blood and the types of blood. Groups without contamination presented higher bond strengths than contaminated groups. The application mode of the cleansing agents had no influence on bond strength results. There was no statistically significant difference among cleansing agents and they were as effective as a water stream in counteracting the effect of blood contamination.


CONCLUSIONS
Heparinized blood can be used as a contaminant for up to one week, and it is a reliable procedure to standardize the contaminant. The cleansing agents can be used without friction. A water stream is sufficient to remove blood contamination from dental tissues, before the application of a one-step adhesive system.
Tachibana
Arlene
A
Department of Restorative Dentistry, University of Sao Paulo, Sao Paulo, Brazil. arlenetachy@yahoo.com
Castanho
Gisela Muassab
GM
Vieira
Samuel Nilo
SN
Matos
Adriana Bona
AB
D000134:Acid Etching, Dental / Q000379:methods*; D000704:Analysis of Variance; D000891:Anti-Infective Agents, Local; D001769:Blood; D003188:Composite Resins; D003666:Decontamination / Q000379:methods*; D001840:Dental Bonding; D003743:Dental Enamel; D003799:Dental Stress Analysis; D003804:Dentin; D003902:Detergents; D006493:Heparin; D006801:Humans; D008422:Materials Testing; D008963:Molar; D019279:Resin Cements; D033081:Shear Strength; D018709:Statistics, Nonparametric; D014867:Water
D000891:Anti-Infective Agents, Local; D003188:Composite Resins; D003902:Detergents; D019279:Resin Cements; D014867:Water; D006493:Heparin