Real-time visualization of axonal transport in neurons.
The normal function of neurons depends on the integrity of microtubule-dependent transport of cellular materials and organelles to/from their cell bodies or axon terminus. In this chapter, we describe the design and implementation of a fluorescence imaging method to visualize axonal transport in neurons directly. We combine a pseudo total internal reflection microscopy, quantum dot fluorescence labeling, microfluidic neuronal culture chamber, and single molecule detection methods to achieve a high spatial and temporal resolution in tracking nerve growth factor transport in dorsal root ganglia neurons.
Department of Chemistry, Stanford University, Stanford, CA, USA.
Yasuko
Y
Osakada
Bianxiao
B
Cui
D000818:Animals; D001370:Axonal Transport / Q000502:physiology*; D002478:Cells, Cultured; D005260:Female; D008853:Microscopy; D009414:Nerve Growth Factors / Q000378:metabolism; D009474:Neurons / Q000378:metabolism*; D011247:Pregnancy; D045663:Quantum Dots; D051381:Rats
D009414:Nerve Growth Factors