Chlorophenol stress affects aromatic amino acid biosynthesis-a genome-wide study.
Chlorophenols are a class of chemicals commonly used in preservatives, disinfectants, algaecides, herbicides and pesticides. However, there is a growing evidence that these compounds are a threat to human health. This is alarming as many chlorophenols are common pollutants found in the global environment at potentially biohazardous levels. Despite chlorophenols being abundant, widely used and poisonous, we know relatively little about their mechanism of toxicity in eukaryotes. Thus, we performed genome-wide growth screens using Saccharomyces cerevisiae to understand the molecular basis of chlorophenol toxicity. Of ∼4850 single gene knockout strains tested, 393 mutants showed growth sensitivity to treatment with 4-chlorophenol (4-CP), 2,4-dichlorophenol (2,4-DCP) or pentachlorophenol (PCP). Only eight mutants showed growth hypersensitivity to all the three treatments and harboured deletions in genes important for aromatic amino acid biosynthesis (ARO1, ARO7) or mitochondrial protein synthesis and respiration (ATP5, ISA1, RML2, GET2, SLS1, MRPL38).
Institute of Genomics and Integrative Biology (IGIB), CSIR, Delhi, India.
Vikas
V
Yadav
Kirti
K
Shitiz
Rishi
R
Pandey
Jyoti
J
Yadav
D024322:Amino Acids, Aromatic / Q000096:biosynthesis*; D002733:Chlorophenols / Q000633:toxicity*; D004202:Disinfectants / Q000378:metabolism; D004785:Environmental Pollutants / Q000378:metabolism / Q000633:toxicity; D016681:Genome, Fungal; D006540:Herbicides / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000096:biosynthesis*; D010575:Pesticides / Q000378:metabolism; D010641:Phenotype; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics; D017384:Sequence Deletion
D024322:Amino Acids, Aromatic; D002733:Chlorophenols; D004202:Disinfectants; D004785:Environmental Pollutants; D006540:Herbicides; D024101:Mitochondrial Proteins; D010575:Pesticides