[Experimental study of laparoscopical lumbar interbody fusion with polyporus composite phosphate calcium and rhBMP-2 compounds in sheep].
OBJECTIVE
To study the efficacy of polyporus composite phosphate calcium and rhBMP-2 compounds with laparoscopical lumbar interbody fusion in sheep.


METHODS
Fourteen uniform-weight adult sheep were randomly divided into three groups for LA-5 interbody fusion with titanium mesh. Autogenous bone and titanium mesh was applied with open anterior technique in group 1 (n=4). In group 2, 4 sheep were operated with laparoscope technique for LA-5 interbody fusion with composite phosphate calcium (CPC) and titanium mesh. In group 3, 6 sheep underwent laparoscopical L4-5 interbody fusion with titanium mesh as well as polyporus composite phosphate calcium and rhBMP-2 compounds. At Weeks 6 and 12 post-operation, the sheep were sacrificed for imaging, biomechanic and morphological examinations.


RESULTS
Although there was no statistical difference between open and laparoscopical interbody fusion group when comparing the remaining disc and endplate decorticated, bone fusion occurred in 3 groups after 3 months. A much larger amount of bony callus was observed earlier in laparoscopical L4-5 interbody fusion group with titanium mesh as well as polyporus composite phosphate calcium and rhBMP-2 compounds than two other groups.


CONCLUSIONS
Polyporus composite phosphate calcium and rhBMP-2 compounds are suitable prosthetic materials for clinical trials. When these materials are utilized with a laparoscopical technique, satisfactory interbody fusion may be achieved.
Department of Orthopedics, Second People's Hospital, Linhai, China.
Zhi-hai
ZH
Zhu
Peng
P
Cao
Yu
Y
Liang
Yao-cheng
YC
Gong
Tao
T
Zheng
Xing-kai
XK
Zhang
Wen-jian
WJ
Wu
D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000627:therapeutic use*; D018786:Bone Substitutes / Q000627:therapeutic use; D002130:Calcium Phosphates / Q000627:therapeutic use*; D010535:Laparoscopy; D008159:Lumbar Vertebrae / Q000601:surgery*; D008297:Male; D011994:Recombinant Proteins / Q000627:therapeutic use; D012756:Sheep; D013123:Spinal Fusion / Q000379:methods*; D054457:Tissue Scaffolds; D014025:Titanium / Q000627:therapeutic use
D055396:Bone Morphogenetic Protein 2; D018786:Bone Substitutes; D002130:Calcium Phosphates; D011994:Recombinant Proteins; C020243:calcium phosphate; D014025:Titanium