Antibody-conjugated gold-gold sulfide nanoparticles as multifunctional agents for imaging and therapy of breast cancer.
The goal of this study was to develop near-infrared (NIR) resonant gold-gold sulfide nanoparticles (GGS-NPs) as dual contrast and therapeutic agents for cancer management via multiphoton microscopy followed by higher intensity photoablation. We demonstrate that GGS-NPs exposed to a pulsed, NIR laser exhibit two-photon induced photoluminescence that can be utilized to visualize cancerous cells in vitro. When conjugated with anti-HER2 antibodies, these nanoparticles specifically bind SK-BR-3 breast carcinoma cells that over-express the HER2 receptor, enabling the cells to be imaged via multiphoton microscopy with an incident laser power of 1 mW. Higher excitation power (50 mW) could be employed to induce thermal damage to the cancerous cells, producing extensive membrane blebbing within seconds leading to cell death. GGS-NPs are ideal multifunctional agents for cancer management because they offer the ability to pinpoint precise treatment sites and perform subsequent thermal ablation in a single setting.
Day
Emily S
ES
Department of Bioengineering, Rice University, Houston, TX, USA.
Bickford
Lissett R
LR
Slater
John H
JH
Riggall
Nicholas S
NS
Drezek
Rebekah A
RA
West
Jennifer L
JL
D000912:Antibodies, Neoplasm / Q000008:administration & dosage / Q000627:therapeutic use*; D001943:Breast Neoplasms / Q000175:diagnosis* / Q000628:therapy*; D045744:Cell Line, Tumor; D005260:Female; D006046:Gold; D006801:Humans; D066298:In Vitro Techniques; D007259:Infrared Rays / Q000627:therapeutic use; D053685:Laser Therapy; D007834:Lasers; D053768:Metal Nanoparticles / Q000008:administration & dosage / Q000627:therapeutic use* / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D036641:Microscopy, Fluorescence, Multiphoton; D050997:Nanomedicine; D010316:Particle Size
D000912:Antibodies, Neoplasm; D006046:Gold