A comparison of breast cancer tumor cells with varying expression of the Her2/neu receptor by Raman microspectroscopic imaging.
The Her2/neu proto-oncogene is amplified in 25 to 30 percent of human primary breast carcinomas. The roles of Her2/neu have been reported before in literature, showing different relations to intracellular lipid composition. Here, we use Raman microspectroscopic imaging to reveal the chemical composition of single live cells from breast carcinoma cell lines MDA-MB-231, MDA-MB-435s and SK-BR-3, which express Her2/neu receptor in different extent. Average Raman spectra of the different cell populations show prominent lipid presence in all cell lines. With high significance, Raman difference spectra reveal increased lipid contents, as well as a lower degree of fatty acid saturation in the MDA-MB cell lines with respect to the SK-BR-3 cells. These results are confirmed by hierarchical cluster analysis of single cells. High internal consistency of the chemical compositions in the cell lines is shown by hierarchical cluster analysis on a single matrix composed of the data of different cells from a single cell line. Although Her2/neu expression is highest for SK-BR-3 cells, their lipid contents are lower than that of the MDA-MB cell lines, which express less to no Her2/neu receptors. Rather than metabolic rate or senescence, the degree of metastaticity of the cells appears to be related to the polyunsaturated fatty acid contents of the cells.
Medical Cell BioPhysics, MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, P.O. Box 217, 7500AE Enschede, The Netherlands.
Liesbeth
L
Hartsuiker
Nicole J L
NJ
Zeijen
Leon W M M
LW
Terstappen
Cees
C
Otto
D001943:Breast Neoplasms / Q000737:chemistry* / Q000473:pathology*; D045744:Cell Line, Tumor; D016000:Cluster Analysis; D005227:Fatty Acids / Q000032:analysis; D005260:Female; D006801:Humans; D008853:Microscopy / Q000379:methods*; D009362:Neoplasm Metastasis; D000090063:Proto-Oncogene Mas; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000032:analysis*; D013059:Spectrum Analysis, Raman / Q000379:methods*
D005227:Fatty Acids; D000090063:Proto-Oncogene Mas; D018719:Erb-b2 Receptor Tyrosine Kinases; C000715470:MAS1 protein, human