Protective effect of a new amide compound from Pu-erh tea on human micro-vascular endothelial cell against cytotoxicity induced by hydrogen peroxide.
A new amide, N-(3,4-dihydroxybenzoyl)-3,4-dihydroxybenzamide (1), was isolated from the Pu-erh tea made with the leaf of Camellia assamica (Mast.) Chang by special fermentation techniques with bacteria and fungus. Its structure was established by means of spectroscopic data analyses, including mass spectrometry and both 1D and 2D NMR spectroscopy. Human micro-vascular endothelial cell (HMEC) injured with H(2)O(2) was used as the model to test protective effect of compound 1 in contrast with other known compounds isolated from Pu-erh tea. These results suggested that compound 1 is a very useful compound to prevent H(2)O(2)-induced cell death of HMEC.
State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191, PR China.
Liang
L
Zhang
Zhi-Zhong
ZZ
Ma
Yan-Yun
YY
Che
Ning
N
Li
Peng-Fei
PF
Tu
D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D001419:Bacteria; D001549:Benzamides / Q000302:isolation & purification / Q000494:pharmacology*; D028241:Camellia sinensis / Q000737:chemistry* / Q000382:microbiology; D016923:Cell Death / Q000187:drug effects*; D042783:Endothelial Cells / Q000187:drug effects*; D005285:Fermentation; D005516:Food Microbiology; D005658:Fungi; D006801:Humans; D006861:Hydrogen Peroxide; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry / Q000382:microbiology; D013662:Tea / Q000382:microbiology*
D000975:Antioxidants; D001549:Benzamides; C560468:N-(3,4-dihydroxybenzoyl)-3,4-dihydroxybenzamide; D010936:Plant Extracts; D013662:Tea; D006861:Hydrogen Peroxide