EGFR inhibitor C225 increases the radiosensitivity of human lung squamous cancer cells.
BACKGROUND
The purpose of the present study is to investigate the direct biological effects of the epidermal growth factor receptor (EGFR) inhibitor C225 on the radiosensitivity of human lung squamous cancer cell-H520. H520 cells were treated with different dosage of 60Co γ ray irradiation (1.953 Gy/min) in the presence or absence of C225. The cellular proliferation, colony forming capacity, apoptosis, the cell cycle distribution as well as caspase-3 were analyzed in vitro.


RESULTS
We found that C225 treatment significantly increased radiosensitivity of H-520 cells to irradiation, and led to cell cycle arrest in G1 phase, whereas 60Co γ ray irradiation mainly caused G2 phase arrest. H-520 cells thus displayed both the G1 and G2 phase arrest upon treatment with C225 in combination with 60Co γ ray irradiation. Moreover, C225 treatment significantly increased the apoptosis percentage of H-520 cells (13.91% ± 1.88%) compared with the control group (5.75% ± 0.64%, P < 0.05).


CONCLUSIONS
In this regard, C225 treatment may make H-520 cells more sensitive to irradiation through the enhancement of caspase-3 mediated tumor cell apoptosis and cell cycle arrest.
Zhang
Yingdong
Y
Department of Radiation Oncology, Cancer center, Peking University Third Hospital, Beijing, P,R, China 100191. doctorwangjunjie@yahoo.com.cn.
Wang
Junjie
J
Liu
Feng
F
You
Zhenyu
Z
Yang
Ruijie
R
Zhao
Yong
Y