Characterization of 26 Staphylococcus warneri isolates from orthopedic infections.
Staphylococcus warneri is a coagulase negative Staphylococcus (CNS) commonly present in the flora of human epithelia and mucosal membranes. Over the last two decades, similarly to other CNS species, S. warneri has been reported as a new emerging pathogen, capable of causing serious infections usually in association with the presence of implant materials, but, at times, even in the absence of a foreign body and in patients considered immunocompetent. At present, there is still a lack of scientific data on the pathogenesis and epidemiology of this species. The present study investigated a collection of 26 clinical isolates derived from orthopedic infections, some associated with implant materials and others not. Automated ribotyping showed the existence of 5 distinct ribogroups. Except for the least numerous ribogroup consisting of a single isolate, all other ribogroups included at least one strain obtained from an infection not associated with implant materials, suggesting that putative virulence factors necessary for infections even in the absence of a foreign body could be transversal to most ribogroup categories. Orthopedic infections were found to involve S. warneri strains with low antibiotic resistance potential, differing in this respect from the strains isolated at neonatal intensive care units, where this species has been described to figure among the principal causative agents and exhibit an alarming profile of antibiotic resistance.
Research Unit on Implant Infections, Rizzoli Orthopedic Institute, Bologna, Italy.
Davide
D
Campoccia
Lucio
L
Montanaro
Livia
L
Visai
Tolmino
T
Corazzari
Claudio
C
Poggio
Francesco
F
Pegreffi
Alessandra
A
Maso
Valter
V
Pirini
Stefano
S
Ravaioli
Ilaria
I
Cangini
Pietro
P
Speziale
Carla Renata
CR
Arciola
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D004269:DNA, Bacterial / Q000302:isolation & purification; D052978:Disk Diffusion Antimicrobial Tests; D024881:Drug Resistance, Bacterial; D006801:Humans; D019637:Orthopedic Procedures / Q000009:adverse effects* / Q000295:instrumentation; D016459:Prosthesis-Related Infections / Q000188:drug therapy / Q000382:microbiology*; D012335:RNA, Ribosomal / Q000302:isolation & purification; D021521:Ribotyping; D013210:Staphylococcus / Q000145:classification* / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity; D013530:Surgical Wound Infection / Q000188:drug therapy / Q000382:microbiology*; D014774:Virulence
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D012335:RNA, Ribosomal