GLOBE: Analysis of DNA-protein interaction analysis.
Emulsion PCR, a hyper, multi, parallel PCR in water-phase droplets in water-in-oil (w/o) emulsion, can be used to make a genetic l ibrary on beads (GLOBE). In GLOBE, we have developed a novel high-throughput screening system for the analysis of the recognition sequences of DNA-binding proteins, which can be prepared by using either an in vivo or an in vitro protein synthesis system. The system can contribute to the low-cost comprehensive analysis of transcription factor-binding regions.
Kojima
Takaaki
T
Laboratory of Molecular Biotechnology, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Japan.
Nakano
Hideo
H
D001483:Base Sequence; D004247:DNA / Q000378:metabolism*; D017931:DNA Primers; D016133:Polymerase Chain Reaction; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism*
D017931:DNA Primers; D011506:Proteins; D004247:DNA