Cigarette smoke affects ABCAl expression via liver X receptor nuclear translocation in human keratinocytes.
Cutaneous tissue is the first barrier against outdoor insults. The outer most layer of the skin, the stratum corneum (SC), is formed by corneocytes embedded in a lipid matrix (cholesterol, ceramide and fatty acids). Therefore, the regulation of lipids and, in particular, of cholesterol homeostasis in the skin is of great importance. ABCA1 is a membrane transporter responsible for cholesterol efflux and plays a key role in maintaining cellular cholesterol levels. Among the many factors that have been associated with skin diseases, the environmental stressor cigarette smoke has been recently studied. In the present study, we demonstrate that ABCA1 expression in human cells (HaCaT) was increased (both mRNA and protein levels) after CS exposure. This effect was mediated by the inhibition of NFkB (aldehydes adducts formation) that allows the translocation of liver X receptor (LXR). These findings suggest that passive smoking may play a role in skin cholesterol levels and thus affect cutaneous tissues functions.
Department of Biomedical Sciences, University of Siena, Via Aldo Moro, 2 53100 Siena, Italy; E-Mails: sticozzi@unisi.it (C.S.); belmonte4@unisi.it (G.B.).
Claudia
C
Sticozzi
Alessandra
A
Pecorelli
Giuseppe
G
Belmonte
Giuseppe
G
Valacchi
D064286:ATP Binding Cassette Transporter 1 / Q000235:genetics / Q000378:metabolism*; D021581:Active Transport, Cell Nucleus; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects* / Q000378:metabolism; D000071518:Liver X Receptors; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism; D057093:Orphan Nuclear Receptors / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014028:Tobacco Smoke Pollution / Q000009:adverse effects*
C577836:ABCA1 protein, human; D064286:ATP Binding Cassette Transporter 1; D000071518:Liver X Receptors; D016328:NF-kappa B; D057093:Orphan Nuclear Receptors; D012333:RNA, Messenger; D014028:Tobacco Smoke Pollution