Detection and quantitation of radiolabeled proteins in gels and blots.
This unit provides procedures for detecting an quantifying radiolabeled proteins in SDS-polyacrylamide gels or blots. Autoradiography requires fixed, dried gels before exposure to X-ray film. The results may be enhanced by using intensifying screens and/or preflashing the film. Alternatively fluorography with organic scintillant-impregnated gels may be used to enhance signal. The autoradiogram may be quantified by densitometry. Phosphorimaging is a useful alternative to film exposure.
Voytas
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D
Iowa State University, Ames, Iowa, USA.
Ke
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N
D001345:Autoradiography; D015153:Blotting, Western; D004586:Electrophoresis; D015336:Molecular Probe Techniques; D011506:Proteins / Q000032:analysis* / Q000737:chemistry*; D011868:Radioisotopes; D011874:Radiometry / Q000379:methods*; D014116:Toxicology / Q000379:methods
D011506:Proteins; D011868:Radioisotopes