Surgical intrauterine insemination with cat semen cryopreserved with Orvus ES paste or sodium lauryl sulfate.
The mean post-thaw sperm motilities of feline frozen semen prepared with 1% OEP or 3 g/ml SLS as a cryoprotective agent, in addition to 7% glycerin, were 35.0 ± 2.4 and 37.0 ± 2.5%, respectively, showing no significant difference. On unilateral intrauterine insemination (UIUI) using these semen samples at a sperm number of 40 × 10(6), the conception rate was 70.0% (7/10) in the OEP group and 30% (3/10) in the SLS group, showing that the rate was higher in the OEP group, but the difference was not significant. It was suggested that sperm in frozen semen showing the above qualities were transferred to the contralateral uterine horn on UIUI.
Department of Reproduction, Nippon Veterinary and Life Science University, Nippon, Japan. tsutsui@nvlu.ac.jp
Toshihiko
T
Tsutsui
Tatsuji
T
Mizutani
Yuka
Y
Matsubara
Mari
M
Toyonaga
Hiromichi
H
Oba
Tatsuya
T
Hori
D000818:Animals; D000831:Animals, Newborn; D002415:Cats / Q000502:physiology*; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary*; D003451:Cryoprotective Agents / Q000494:pharmacology*; D005260:Female; D007315:Insemination, Artificial / Q000379:methods / Q000662:veterinary*; D008098:Litter Size; D008297:Male; D011247:Pregnancy; D012661:Semen; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology*
D003451:Cryoprotective Agents; D012967:Sodium Dodecyl Sulfate