Ionic-liquid-based catch and release mass spectroscopy tags for enzyme monitoring.
A novel, inexpensive and versatile ionic-liquid-based catch and release mass spectrometry tag (I-Tag) that facilitates substrate purification, fast, robust and sensitive enzymatic reaction monitoring and quantitative kinetic analysis has been developed. The applicability of the system has been demonstrated in an enzymatic assay with β-1,4-galactosyltransferase.
Galan
M Carmen
MC
School of Chemistry, University of Bristol, Cantock's Close, Bristol, BS8 1TS, UK. M.C.Galan@bristol.ac.uk
Tran
Anh Tuan
AT
Bernard
Claire
C
D002853:Chromatography, Liquid; D052578:Ionic Liquids / Q000737:chemistry*; D007700:Kinetics; D013058:Mass Spectrometry; D009237:N-Acetyllactosamine Synthase / Q000737:chemistry* / Q000378:metabolism
D052578:Ionic Liquids; D009237:N-Acetyllactosamine Synthase