The influence of biocomposites containing genetically modified flax fibers on gene expression in rat skeletal muscle.
In many studies, natural flax fibers have been proven to be resistant and surgically suitable. Genetically modified flax fibers, derived from transgenic flax expressing three bacterial genes for the synthesis of poly-3-hydroxybutyric acid (PHB), have better mechanical properties than unmodified flax fibers. The aim of this study was to examine the biocompatibility of composites containing flax fibers from transgenic polyhydroxybutyrate producing (M50) and control (wt-NIKE) plants in a polylactide (PLA) matrix in rat Musculus latissimus dorsi. For this purpose, effects of biocomposites on the expression of growth factors and osteogenic differentiation, in particular the mRNA expression of vascular endothelial growth factor, insulin like growth factor 1, insulin like growth factor 2, collagen-1, collagen-2 and myostatin, were analyzed using quantitative RT-PCR. The biocomposites did not show any inflammation response after subcutaneous insertion. The results following subcutaneous insertion of PLA alone and PLA-M50 showed no significant changes on the gene expression of all tested genes, whereas PLA-wt-NIKE reduced the mRNA amount of myostatin, VEGFA and IGF2, respectively. It can be asserted that modified flax membranes with PHB and other organic substances have a good biocompatibility to the muscle and they do not disrupt the muscle function. Furthermore, composites from transgenic flax plants producing PHB did not differ from composites of non-transgenic flax plants.
Gredes
Tomasz
T
Department of Orthodontics, Preventive and Pediatric Dentistry, Ernst-Moritz-Arndt University Greifswald, Greifswald, Germany. thomasgredes@yahoo.de
Kunert-Keil
Christiane
C
Dominiak
Marzena
M
Gedrange
Tomasz
T
Wróbel-Kwiatkowska
Magdalena
M
Szopa
Jan
J
D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D019597:Flax / Q000737:chemistry* / Q000235:genetics*; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology*; D008422:Materials Testing; D009124:Muscle Proteins / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000187:drug effects / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000737:chemistry*; D000091402:Prohibitins; D051381:Rats; D011917:Rats, Inbred Lew
D001672:Biocompatible Materials; D009124:Muscle Proteins; C000717332:Phb protein, rat; D000091402:Prohibitins