A matrix computational approach to kinesin neck linker extension.
Kinesin stepping requires both tethered diffusion of the free head and conformational changes driven by the chemical state of the motor. We present a numerical method using matrix representations of approximating Markov chains and renewal theory to compute important experimental quantities for models that include both tethered diffusion and chemical transitions. Explicitly modeling the tethered diffusion allows for exploration of the model under perturbation of the neck linker; comparisons are made between the computed models and in vitro assays.
Department of Statistics, The Pennsylvania State University, University Park, PA 16802, United States. jph264@psu.edu
John
J
Hughes
William O
WO
Hancock
John
J
Fricks
D000255:Adenosine Triphosphate / Q000378:metabolism; D019295:Computational Biology / Q000379:methods*; D004058:Diffusion; D006801:Humans; D016547:Kinesins / Q000737:chemistry* / Q000378:metabolism; D007700:Kinetics; D008390:Markov Chains; D008954:Models, Biological
D000255:Adenosine Triphosphate; D016547:Kinesins