In vivo inhibition of NAS preparation on H9N2 subtype AIV.
NAS preparation, a kind of Chinese herbal medicine found by the Yunnan Eco-agricultural Research Institute, has potential antiviral activity. In this paper, the inhibiting effect of NAS preparation on H9N2 subtype Avian influenza virus (AIV) was investigated in vivo. Chickens infected with H9N2 virus were treated with NAS preparation for 4 days. The virus was then detected by hemoagglutination (HA) test and reverse transcription polymerase chain reaction (RT-PCR). The results showed that no H9N2 virus could be detected at the 7th day when the chickens were treated with 0.2 g/kg/d or 0.1 g/kg/d of NAS preparation. However the virus could be detected in other chickens without NAS preparation treatment. This result suggested that NAS preparation may be a potential drug candidate to control infection of H9N2 subtype AIV in chickens.
Key Laboratory of New Animal Medicine Project, Chinese Academy of Agricultural Sciences, Lanzhou, China.
Ruo-feng
RF
Shang
Jian-ping
JP
Liang
Zhong-yuan
ZY
Na
Hong-jun
HJ
Yang
Yu
Y
Lu
Lan-ying
LY
Hua
Wen-zhu
WZ
Guo
Ying
Y
Cui
Ling
L
Wang
D000818:Animals; D000998:Antiviral Agents / Q000008:administration & dosage*; D002645:Chickens; D002988:Cloaca / Q000821:virology; D006386:Hemagglutination Tests; D053127:Influenza A Virus, H9N2 Subtype / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D005585:Influenza in Birds / Q000188:drug therapy* / Q000821:virology; D010614:Pharynx / Q000821:virology; D010936:Plant Extracts / Q000008:administration & dosage*; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors
D000998:Antiviral Agents; D010936:Plant Extracts; D012367:RNA, Viral; C015378:nas