Methodological contributions towards LC-MS/MS quantification of free VX in plasma: An innovative approach.
A novel analytical method has been developed to detect and quantify VX (O-ethyl S-(2(diisopropylamino) ethyl) (methylphosphonothioate)) in plasma using an LC-MS/MS technique. VX detection and quantification in plasma following percutaneous exposure represent a formidable challenge and it is an important part of the ongoing struggle against chemical warfare agents. Liquid-liquid extraction of VX from plasma was performed and it generated a recovery rate of approximately 65% followed by an LC-MS/MS analysis in a 100% organic phase. An Allure biphenyl column (Restek) was tested with detection limit at 0.5 pg/mL (5 μL injected). Initial application was focused on human skin grafted on nude mice as an experimental model with proper adjustments done for very small quantities of plasma.
Debouit
Charlotte
C
I.R.B.A., Antenne C.R.S.S.A., Service de Biospectrométrie, 24 Avenue des Maquis du Grésivaudan, BP 87-F 38702 La Tronche Cedex, France. cdebouit@hotmail.fr
Bazire
Alexandre
A
Lallement
Guy
G
Daveloose
Denis
D
D002619:Chemical Warfare Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D009946:Organothiophosphorus Compounds / Q000097:blood*; D053719:Tandem Mass Spectrometry / Q000379:methods*
D002619:Chemical Warfare Agents; D009946:Organothiophosphorus Compounds; C009680:VX-agent