Mass mortality associated with koi herpesvirus in wild common carp in Canada.
Koi herpesvirus (KHV) was identified as being associated with more than one mortality event affecting common carp in Canada. The first was an extensive mortality event that occurred in 2007 in the Kawartha Lakes region, Ontario, affecting Lakes Scugog and Pigeon. Fish had branchial necrosis and hepatic vasculitis with an equivocal interstitial nephritis. Several fish also had branchial columnaris. Subsequent mortality events occurred in 2008 in additional bodies of water in south-central Ontario, such as Lake Katchewanooka and outside of Ontario in Lake Manitoba, Manitoba. Koi herpesvirus was detected in fish submitted for examination from all of these lakes by polymerase chain reaction (PCR), and sequence of the PCR product revealed 100% homology to KHV strains U and I. Real-time PCR analysis of KHV-infected wild carp revealed viral loads ranging from 6.02×10(1) to 2.4×10(6) copies μg(-1) host DNA. This is the first report of KHV in Canada.
Garver
Kyle A
KA
Fisheries & Oceans Canada, Pacific Biological Station, 3190 Hammond Bay Road, Nanaimo, British Colombia V9T 6N7, Canada. kyle.garver@dfo-mpo.gc.ca
Al-Hussinee
Lowia
L
Hawley
Laura M
LM
Schroeder
Tamara
T
Edes
Sandra
S
LePage
Veronique
V
Contador
Elena
E
Russell
Spencer
S
Lord
Stephen
S
Stevenson
Roselynn M W
RM
Souter
Brian
B
Wright
Elizabeth
E
Lumsden
John S
JS
D000818:Animals; D002170:Canada / Q000453:epidemiology; D002347:Carps / Q000821:virology*; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D005393:Fish Diseases / Q000453:epidemiology / Q000401:mortality*; D006564:Herpesviridae / Q000235:genetics / Q000302:isolation & purification; D006566:Herpesviridae Infections / Q000453:epidemiology / Q000401:mortality / Q000662:veterinary*; D008297:Male; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D019562:Viral Load / Q000662:veterinary
D004279:DNA, Viral