Serum 14-3-3; matrix metalloproteinases and their inhibitors' levels before and after cardiovascular surgery in diabetic and non-diabetic patients.
OBJECTIVE
We sought to assess the serum levels of the main extracellular matrix components before and after surgery in order to differentiate the response of diabetic patients to acute wounds from that of non-diabetic patients.


METHODS
The serum levels of 14-3-3, Pro-MMP-1, MMP-3, and TIMP-1 were measured in diabetics (18 patients) and non-diabetics (22 patients) in samples obtained before a coronary artery bypass grafting operation and on the 1st, 3rd, and 5th postoperative days.


RESULTS
The diabetics had higher serum levels of 14-3-3 both in the pre- and postoperative phases. Nevertheless, there was a postoperative drop in these amounts in all the patients. There was no difference in the serum levels of Pro-MMP-1 between the two groups. In addition, the serum levels of MMP-3 on the 3rd and 5th postoperative days and also TIMP-1 (inhibitor of both MMPs) on all postoperative days were higher in the diabetics.


CONCLUSIONS
There was perfect synchronicity between the changes in the serum levels of these proteins and their functional nature in the injured tissue. Furthermore, the diabetic patients exhibited more changes in the levels of some of their extracellular enzymes in the wake of acute wounds; these changes were also traceable in the serum.
Endocrinology and Metabolism Research center, Tehran University of Medical Sciences, Tehran, Iran.
Alireza
A
Mohajerani
Aziz
A
Ghahary
Mohammadreza
M
Khuramizadeh
Bagher
B
Larijani
D048948:14-3-3 Proteins / Q000097:blood*; D000328:Adult; D001026:Coronary Artery Bypass; D003924:Diabetes Mellitus, Type 2 / Q000097:blood*; D006801:Humans; D008297:Male; D020782:Matrix Metalloproteinases / Q000097:blood*; D008875:Middle Aged; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000097:blood; D014945:Wound Healing / Q000502:physiology
D048948:14-3-3 Proteins; D019715:Tissue Inhibitor of Metalloproteinase-1; D020782:Matrix Metalloproteinases