Detection of Chlamydophila psittaci by using SYBR green real-time PCR.
Chlamydophila psittaci is the causative agent of human psittacosis and avian chlamydiosis. This zoonotic pathogen is frequently transmitted from infected birds to humans. Therefore proper and rapid detection of C. psittaci in birds is important to control this disease. We developed a method for detecting C. psittaci by using SYBR Green Real-time PCR based on targeting the cysteine-rich protein gene (envB) of C. psittaci. This one step procedure was highly sensitive and rapid for detection and quantification of C. psittaci from fecal samples. This assay was also able to detect other zoonotic Chlamydophila species such as C. abortus and C. felis. The assay is well suited for use as a routine detection method in veterinary medicine.
Department of Applied Veterinary Sciences, Gifu University, Gifu, Japan.
Hideko
H
Okuda
Kenji
K
Ohya
Yukihiro
Y
Shiota
Hiroshi
H
Kato
Hideto
H
Fukushi
D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000235:genetics; D001483:Base Sequence; D001715:Bird Diseases / Q000175:diagnosis / Q000382:microbiology*; D001717:Birds; D002690:Chlamydia Infections / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D002691:Chlamydophila psittaci / Q000235:genetics / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005243:Feces / Q000382:microbiology; D006801:Humans; D008969:Molecular Sequence Data; D009930:Organic Chemicals / Q000737:chemistry*; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D012680:Sensitivity and Specificity; D015047:Zoonoses / Q000382:microbiology*
D001425:Bacterial Outer Membrane Proteins; D004269:DNA, Bacterial; C063931:Omp2 protein, bacteria; D009930:Organic Chemicals; C098798:SYBR Green II