Voltammetric detection of biological molecules using chopped carbon fiber.
Voltammetric detection of biological molecules was carried out using chopped carbon fibers produced from carbon fiber reinforced plastics that are biocompatible and inexpensive. Because chopped carbon fibers normally are covered with a sizing agent, they are difficult to use as an electrode. However, when the surface of a chopped carbon fiber was treated with ethanol and hydrochloric acid, it became conductive. To evaluate the functioning of chopped carbon fibers, voltammetric measurements of [Fe(CN)(6)](3-) were carried out. Redoxes of FAD, ascorbic acid and NADH as biomolecules were recorded using cyclic voltammetry. The sizing agents used to bundle the fibers were epoxy, polyamide and polyurethane resins. The peak currents were the greatest when using the chopped carbon fibers that were created with epoxy resins. When the electrode response of the chopped carbon fibers was compared with that of a glassy carbon electrode, the peak currents and the reversibility of the electrode reaction were sufficient. Therefore, the chopped carbon fibers will be useful as disposable electrodes for the sensing of biomolecules.
Sugawara
Kazuharu
K
Faculty of Engineering, Maebashi Institute of Technology, Maebashi, Gunma 371-0816, Japan. kzsuga@maebashi-it.ac.jp
Yugami
Asako
A
Kojima
Akira
A
D001205:Ascorbic Acid / Q000032:analysis*; D002244:Carbon; D000077482:Carbon Fiber; D005182:Flavin-Adenine Dinucleotide / Q000032:analysis*; D009243:NAD / Q000032:analysis*; D010084:Oxidation-Reduction; D010969:Plastics; D011199:Potentiometry
D000077482:Carbon Fiber; D010969:Plastics; C037808:plastic, carbon fiber reinforced; D009243:NAD; D005182:Flavin-Adenine Dinucleotide; D002244:Carbon; D001205:Ascorbic Acid