Rational strategy of magnetic relaxation switches for glycoprotein sensing.
A rational strategy of magnetic relaxation switches was proposed here to detect a(1)-acid glycoprotein (AGP), an acute phase a-globulin plasma glycoprotein. The assay was based on the relaxation time change between the aggregation of magnetic nanoparticles with concanavalin A and the redispersion with AGP, which can avoid the prozone effect and improve the detection accuracy. The assay was an easy and efficient method with two mixing steps and one measurement step, showing a detection limit of 0.66 nM in 0~0.3 μg mL(-1) AGP, which was far lower than its normal level in human plasma.
Cai
Shaoyu
S
Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai, P.R. China.
Liang
Guohai
G
Zhang
Peng
P
Chen
Hui
H
Zhang
Song
S
Liu
Baohong
B
Kong
Jilie
J
D015374:Biosensing Techniques / Q000379:methods*; D003208:Concanavalin A / Q000737:chemistry; D005290:Ferric Compounds / Q000737:chemistry; D006023:Glycoproteins / Q000097:blood*; D006801:Humans; D008280:Magnetics; D053758:Nanoparticles / Q000737:chemistry
D005290:Ferric Compounds; D006023:Glycoproteins; D003208:Concanavalin A; C000499:ferric oxide