Interferons induce CXCR3-cognate chemokine production by human metastatic melanoma.
Immune-mediated cancer regression requires tumor infiltration by antigen-specific effector T cells, but lymphocytes are commonly sparse in melanoma metastases. Activated T cells express CXCR3, whose cognate chemokines are CXCL9/MIG, CXCL10/IP-10, and CXCL11/I-TAC. Little is known about expression of these chemokines in lymph node (LN) metastases of melanoma. We evaluated whether metastatic melanoma induces these CXCR3-cognate chemokines in human LN-derived tissues. In addition, as these chemokines can be induced by interferon (IFN), we evaluated whether type I or II IFNs (IFN-α or IFN-γ, respectively) can modulate chemokine expression in an in vitro model of the human tumor microenvironment. Production of CXCL9-11 by melanoma-infiltrated nodes (MIN) was no different than tumor-free nodes; both produced less chemokine than activated LN (sentinel immunized nodes, SIN). These data suggest that melanoma infiltration into LN neither induces nor reduces CXCL9-11. Stimulation with IFN-α or IFN-γ increased production of CXCL10-11 from MIN, but not tumor-free node or SIN. IFN-γ also increased production of CXCL9 in MIN. In IFN-treated SIN, CD14+ cells were the primary source of CXCL9-11, whereas melanoma cells were the source of chemokine in MIN. Melanoma cells in MIN express IFN receptors. Consistent with these observations, multiple human melanoma lines expressed IFN receptors and produced CXCL9-11 in response to IFN treatment. Thus, melanoma infiltration of LN is insufficient to induce the production of CXCL9-11, but melanoma may be a significant source of IFN-induced chemokines. Collectively, these data suggest that IFN-α or IFN-γ may act in the tumor microenvironment to increase the chemotactic gradient for CXCR3+ T cells.
Dengel
Lynn T
LT
Human Immune Therapy Center, University of Virginia School of Medicine, Charlottesville, VA, USA.
Norrod
Allison G
AG
Gregory
Briana L
BL
Clancy-Thompson
Eleanor
E
Burdick
Marie D
MD
Strieter
Robert M
RM
Slingluff
Craig L
CL
Mullins
David W
DW
D045744:Cell Line, Tumor; D054357:Chemokine CXCL10 / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D054371:Chemokine CXCL11 / Q000235:genetics / Q000378:metabolism*; D054370:Chemokine CXCL9 / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D007370:Interferon Type I / Q000276:immunology / Q000378:metabolism; D007371:Interferon-gamma / Q000276:immunology / Q000378:metabolism; D008198:Lymph Nodes / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D008207:Lymphatic Metastasis; D008213:Lymphocyte Activation; D008545:Melanoma; D054367:Receptors, CXCR3 / Q000276:immunology / Q000378:metabolism*; D012878:Skin Neoplasms; D013601:T-Lymphocytes; D059016:Tumor Microenvironment
C515187:CXCR3 protein, human; D054357:Chemokine CXCL10; D054371:Chemokine CXCL11; D054370:Chemokine CXCL9; D007370:Interferon Type I; D054367:Receptors, CXCR3; D007371:Interferon-gamma