The prediction of novel multiple lipid-binding regions in protein translocation motor proteins: a possible general feature.
Protein translocation is an important cellular process. SecA is an essential protein component in the Sec system, as it contains the molecular motor that facilitates protein translocation. In this study, a bioinformatics approach was applied in the search for possible lipid-binding helix regions in protein translocation motor proteins. Novel lipid-binding regions in Escherichia coli SecA were identified. Remarkably, multiple lipid-binding sites were also identified in other motor proteins such as BiP, which is involved in ER protein translocation. The prokaryotic signal recognition particle receptor FtsY, though not a motor protein, is in many ways related to SecA, and was therefore included in this study. The results demonstrate a possible general feature for motor proteins involved in protein translocation.
Keller
Rob C A
RC
Section Chemistry, Charlemagne College, Wilhelminastraat 13-15, Nijmegen, 3913 NH, The Netherlands. r.keller@kgcnijmegen.nl
D000251:Adenosine Triphosphatases / Q000737:chemistry* / Q000378:metabolism; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D019295:Computational Biology; D000091342:Endoplasmic Reticulum Chaperone BiP; D004926:Escherichia coli / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000737:chemistry / Q000378:metabolism; D008055:Lipids / Q000737:chemistry*; D026901:Membrane Transport Proteins / Q000737:chemistry* / Q000378:metabolism; D020409:Molecular Motor Proteins / Q000737:chemistry* / Q000378:metabolism; D008969:Molecular Sequence Data; D011485:Protein Binding; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D021381:Protein Transport; D018160:Receptors, Cytoplasmic and Nuclear / Q000737:chemistry / Q000378:metabolism; D000069816:SEC Translocation Channels; D000081416:SecA Proteins
D001426:Bacterial Proteins; D000091342:Endoplasmic Reticulum Chaperone BiP; C087406:FtsY protein, Bacteria; D006360:Heat-Shock Proteins; D008055:Lipids; D026901:Membrane Transport Proteins; D020409:Molecular Motor Proteins; D018160:Receptors, Cytoplasmic and Nuclear; D000069816:SEC Translocation Channels; D000251:Adenosine Triphosphatases; D000081416:SecA Proteins