Transcutaneous immunization with hydrophilic recombinant gp100 protein induces antigen-specific cellular immune response.
The objective of this study was to evaluate the potential of transcutaneous immunization with tumor antigen to induce cell-mediated immunity. For this purpose, hydrophilic recombinant gp100 protein (HR-gp100) was topically applied on human intact skin in vitro, and used as a vaccine in a mouse model. We demonstrate that HR-gp100 permeates into human skin, and is processed and presented by human dendritic cells. In a mouse model, an HR-gp100-based vaccine triggered antigen-specific T cell responses, as shown by proliferation assays, ELISA and intracellular staining for IFN-γ. Transcutaneous antigen delivery may provide a safe, simple and effective method to elicit cell-mediated immunity.
Sharett Institute of Oncology, Hadassah Medical Organization, Jerusalem, Israel.
Galit
G
Eisenberg
Arthur
A
Machlenkin
Shoshana
S
Frankenburg
Adva
A
Mansura
Jacob
J
Pitcovski
Eitan
E
Yefenof
Tamar
T
Peretz
Michal
M
Lotem
D000279:Administration, Cutaneous; D000818:Animals; D017951:Antigen Presentation / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D019496:Cancer Vaccines / Q000737:chemistry / Q000276:immunology; D049109:Cell Proliferation; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism; D004847:Epithelial Cells / Q000378:metabolism; D005260:Female; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D007111:Immunity, Cellular / Q000276:immunology*; D007371:Interferon-gamma / Q000378:metabolism; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology; D008545:Melanoma / Q000276:immunology / Q000517:prevention & control; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D010446:Peptide Fragments; D010455:Peptides / Q000276:immunology; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D017384:Sequence Deletion; D012869:Skin Absorption / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D014611:Vaccination; D058951:gp100 Melanoma Antigen / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology* / Q000378:metabolism
D019496:Cancer Vaccines; C548637:PMEL protein, human; D010446:Peptide Fragments; D010455:Peptides; D011994:Recombinant Proteins; D058951:gp100 Melanoma Antigen; C548641:gp100(280-288) melanoma antigen peptide; D007371:Interferon-gamma