Role of interleukin 17 in arthritis chronicity through survival of synoviocytes via regulation of synoviolin expression.
BACKGROUND
The use of TNF inhibitors has been a major progress in the treatment of chronic inflammation. However, not all patients respond. In addition, response will be often lost when treatment is stopped. These clinical aspects indicate that other cytokines might be involved and we focus here on the role of IL-17. In addition, the chronic nature of joint inflammation may contribute to reduced response and enhanced chronicity. Therefore we studied the capacity of IL-17 to regulate synoviolin, an E3 ubiquitin ligase implicated in synovial hyperplasia in human rheumatoid arthritis (RA) FLS and in chronic reactivated streptococcal cell wall (SCW)-induced arthritis.


RESULTS
Chronic reactivated SCW-induced arthritis was examined in IL-17R deficient and wild-type mice. Synoviolin expression was analysed by real-time RT-PCR, Western Blot or immunostaining in RA FLS and tissue, and p53 assessed by Western Blot. Apoptosis was detected by annexin V/propidium iodide staining, SS DNA apoptosis ELISA kit or TUNEL staining and proliferation by PCNA staining. IL-17 receptor A (IL-17RA), IL-17 receptor C (IL-17-RC) or synoviolin inhibition were achieved by small interfering RNA (siRNA) or neutralizing antibodies. IL-17 induced sustained synoviolin expression in RA FLS. Sodium nitroprusside (SNP)-induced RA FLS apoptosis was associated with reduced synoviolin expression and was rescued by IL-17 treatment with a corresponding increase in synoviolin expression. IL-17RC or IL-17RA RNA interference increased SNP-induced apoptosis, and decreased IL-17-induced synoviolin. IL-17 rescued RA FLS from apoptosis induced by synoviolin knockdown. IL-17 and TNF had additive effects on synoviolin expression and protection against apoptosis induced by synoviolin knowndown. In IL-17R deficient mice, a decrease in arthritis severity was characterized by increased synovial apoptosis, reduced proliferation and a marked reduction in synoviolin expression. A distinct absence of synoviolin expressing germinal centres in IL-17R deficient mice contrasted with synoviolin positive B cells and Th17 cells in synovial germinal centre-like structures.


CONCLUSIONS
IL-17 induction of synoviolin may contribute at least in part to RA chronicity by prolonging the survival of RA FLS and immune cells in germinal centre reactions. These results extend the role of IL-17 to synovial hyperplasia.
Toh
Myew-Ling
ML
Research Unit Immunogenomics and Inflammation, EA 4130, Hospital Edouard Herriot, University of Lyon, Lyon, France.
Gonzales
Gaelle
G
Koenders
Marije I
MI
Tournadre
Anne
A
Boyle
David
D
Lubberts
Erik
E
Zhou
Yuan
Y
Firestein
Gary S
GS
van den Berg
Wim B
WB
Miossec
Pierre
P
D000818:Animals; D001172:Arthritis, Rheumatoid / Q000378:metabolism / Q000473:pathology*; D015153:Blotting, Western; D002908:Chronic Disease; D004797:Enzyme-Linked Immunosorbent Assay; D020287:In Situ Nick-End Labeling; D020381:Interleukin-17 / Q000235:genetics / Q000502:physiology*; D051379:Mice; D034622:RNA Interference; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013583:Synovial Membrane / Q000378:metabolism / Q000473:pathology*; D044767:Ubiquitin-Protein Ligases / Q000378:metabolism*
D020381:Interleukin-17; C498235:Syvn1 protein, mouse; D044767:Ubiquitin-Protein Ligases