Evaluating the effects of immunotoxicants using carbon fiber microelectrode amperometry.
Carbon fiber microelectrode amperometry (CFMA) is explored as a technique for studying the effects of immunotoxicants on single-cell in vitro exocytosis function in a mouse peritoneal mast cell (MPMC)/fibroblast co-culture model. MPMCs were acutely exposed to between 10 and 100 μM of the immunotoxicants mono-2-ethylhexyl phthalate (MEHP) and bisphenol A (BPA), and release of serotonin was evaluated by CFMA. A significant decrease in the quantal content of serotonin was measured for all levels of exposure to both MEHP and BPA. The overall efficiency of the exocytotic function of MPMCs was found to be impaired by all exposure concentrations of BPA, but this efficiency was only impaired at the lowest exposure concentration of MEHP. This study illustrates the potential of CFMA as a technique for determining quantitative and biophysical chemical information in in vitro immunotoxicological studies.
Department of Chemistry, University of Minnesota, 207 Pleasant St. SE, Minneapolis, MN 55418, USA.
Bryce J
BJ
Marquis
Christy L
CL
Haynes
D000818:Animals; D001559:Benzhydryl Compounds; D002244:Carbon / Q000737:chemistry*; D000077482:Carbon Fiber; D018920:Coculture Techniques; D004051:Diethylhexyl Phthalate / Q000031:analogs & derivatives / Q000494:pharmacology; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods; D005089:Exocytosis / Q000187:drug effects; D000922:Immunotoxins / Q000494:pharmacology*; D016014:Linear Models; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D051379:Mice; D008810:Mice, Inbred C57BL; D008839:Microelectrodes; D010636:Phenols / Q000494:pharmacology; D012701:Serotonin / Q000032:analysis / Q000378:metabolism; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D002244:Carbon; D000077482:Carbon Fiber; D004051:Diethylhexyl Phthalate; D000922:Immunotoxins; D010636:Phenols; D012701:Serotonin; C016599:mono-(2-ethylhexyl)phthalate; C006780:bisphenol A; D000098826:Bisphenol A Compounds