Aerobic cometabolic degradation of trichloroethene by methane and ammonia oxidizing microorganisms naturally associated with Carex comosa roots.
The degradation potential of trichloroethene by the aerobic methane- and ammonia-oxidizing microorganisms naturally associated with wetland plant (Carex comosa) roots was examined in this study. In bench-scale microcosm experiments with washed (soil free) Carex comosa roots, the activity of root-associated methane- and ammonia-oxidizing microorganisms, which were naturally present on the root surface and/or embedded within the roots, was investigated. Significant methane and ammonia oxidation were observed reproducibly in batch reactors with washed roots incubated in growth media, where methane oxidation developed faster (2 weeks) compared to ammonia oxidation (4 weeks) in live microcosms. After enrichment, the methane oxidizers demonstrated their ability to degrade 150 μg l(-1) TCE effectively at 1.9 mg l(-1) of aqueous CH(4). In contrast, ammonia oxidizers showed a rapid and complete inhibition of ammonia oxidation with 150 μg l(-1) TCE at 20 mg l(-1) of NH(4)(+)-N, which may be attributed to greater sensitivity of ammonia oxidizers to TCE or its degradation product. No such inhibitory effect of TCE degradation was detected on methane oxidation at the above experimental conditions. The results presented here suggest that microorganisms associated with wetland plant roots can assist in the natural attenuation of TCE in contaminated aquatic environments.
Environmental Science Program, Wright State University, Dayton, OH 45435, USA.
C L
CL
Powell
G
G
Nogaro
A
A
Agrawal
D000332:Aerobiosis; D000641:Ammonia / Q000378:metabolism*; D001419:Bacteria / Q000302:isolation & purification / Q000378:metabolism*; D001673:Biodegradation, Environmental; D031255:Carex Plant / Q000382:microbiology*; D003470:Culture Media / Q000378:metabolism; D008697:Methane / Q000378:metabolism*; D010084:Oxidation-Reduction; D018517:Plant Roots / Q000382:microbiology; D014241:Trichloroethylene / Q000378:metabolism*
D003470:Culture Media; D014241:Trichloroethylene; D000641:Ammonia; D008697:Methane