[Expression of microtubule associated protein-2 mRNA after cerebral contusion].
OBJECTIVE
To explore the mRNA expression of microtubule associated protein-2 (MAP-2) after cerebral contusion, and to investigate its relationship with post-injury interval in a rat model of cerebral contusion.


METHODS
Based on Feeney's model of cerebral contusion, the time-dependent changes of MAP-2 mRNA were detected by real-time PCR method in different groups.


RESULTS
MAP-2 mRNA showed a lower expression in the brain of the control group. The expression of MAP-2 mRNA decreased 1 h post-injury, and reached the lowest level at 6 h, and the expression of MAP-2 mRNA gradually increased in the focus of the cerebral contusion from 12h to 14d after contusion, which kept a high level up to 14 d post-injury.


CONCLUSIONS
The time-dependent changes of MAP-2 mRNA expression may be a new method for estimating the wound age of cerebral contusion in forensic practice.
Liang
Xin-hua
XH
Department of Forensic Pathology, School of Forensic Medicine, Shanxi Medical University, Taiyuan 030001, China. liangxhlemon@163.com
Fu
Jie
J
Sun
Jun-hong
JH
Jiao
Yan
Y
Wang
Ying-yuan
YY
D000818:Animals; D001930:Brain Injuries / Q000378:metabolism*; D003288:Contusions / Q000378:metabolism*; D004195:Disease Models, Animal; D005260:Female; D049429:Forensic Pathology; D008297:Male; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013997:Time Factors
C489787:MAP2 protein, rat; D008869:Microtubule-Associated Proteins; D012333:RNA, Messenger