Genomic damage in the progression of chronic kidney disease in rats.
Patients with chronic renal failure exhibit massive oxidative genome damage and an elevated risk of cancer. Previous studies have demonstrated the relationship between DNA damage and carcinogenesis. The current study aimed to investigate whether the progression of chronic kidney disease induces genomic damage in an animal model. Adult Wistar rats were assigned to either the control or chronic kidney disease groups. The chronic kidney disease group was subdistributed into five groups with progressively longer durations of disease (30, 60, 90, 120 and 150 days). The results showed that chronic kidney disease induced genomic damage in the blood, liver and kidney cells during all periods evaluated, as indicated by the mean tail moment measured in the comet assay. In brain cells, no genetic damage was induced at early/intermediate disease durations; however, positive genotoxicity was found at 120 and 150 days. Blood pressure and pro-inflammatory cytokine levels (IL-1α, IL-1β, IL-6 and TNFα) were increased after chronic kidney disease induction, while blood iron concentration was significantly reduced in these animals. The results suggest that chronic kidney disease progression contributes to DNA damage in blood, liver, kidney and brain and that such damage can be mediated by hypertension, an inflammatory status and iron deficiency. Additionally, the brain was sensitive to genotoxic insult after extended chronic kidney disease, suggesting a potentially important role of genetic damage in the neurological disorders of end-stage renal patients.
Departamento de Psicobiologia, Universidade Federal de Sao Paulo (UNIFESP), Sao Paulo, Brazil.
Camila
C
Hirotsu
Sergio
S
Tufik
Daniel A
DA
Ribeiro
Tathiana A
TA
Alvarenga
Monica L
ML
Andersen
D000704:Analysis of Variance; D000818:Animals; D001794:Blood Pressure / Q000235:genetics; D020552:Comet Assay; D016207:Cytokines / Q000097:blood; D004249:DNA Damage / Q000235:genetics*; D018450:Disease Progression; D007668:Kidney / Q000503:physiopathology*; D007676:Kidney Failure, Chronic / Q000235:genetics* / Q000503:physiopathology; D008099:Liver / Q000503:physiopathology*; D008297:Male; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
D016207:Cytokines