Promoting effects of Ganoderma lucidum polysaccharides on B16F10 cells to activate lymphocytes.
The immune system in patients with cancer often fails to control tumour growth because of deficient immunogenicity of tumour cells. Ganoderma lucidum polysaccharides (Gl-PS) are believed to have anti-tumour effects by boosting host immune function. Additionally, Gl-PS may have some direct effects on tumour cells in the activation of lymphocytes, thus enhancing the immunogenicity of tumour cells. We tested the effects of Gl-PS in lymphocyte activation by incubating Gl-PS with a tumour cell line deficient in antigen presentation. Our study showed that Gl-PS can promote B16F10 melanoma cells to induce lymphocyte proliferation, CD69 and FasL expression and IFN-γ production, indicating that Gl-PS can improve the nature of B16F10 cells to activate lymphocytes. Furthermore, H-2D(b) [a major histocompatibility (MHC) class I molecule], and B7-1 and B7-2 (two prominent co-stimulatory molecules expressed on B16F10 cells) were enhanced by Gl-PS, suggesting that these molecules may at least partially be involved in the process of Gl-PS on B16F10 cells to activate lymphocytes.
Sun
Li-Xin
LX
Department of Pharmacology, Peking University Health Science Center, School of Basic Medical Sciences, Beijing, China.
Lin
Zhi-Bin
ZB
Li
Xue-Jun
XJ
Li
Min
M
Lu
Jie
J
Duan
Xin-Suo
XS
Ge
Zhi-Hua
ZH
Song
You-Xin
YX
Xing
En-Hong
EH
Li
Wei-Dong
WD
D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D000945:Antigens, Differentiation, T-Lymphocyte / Q000378:metabolism; D018122:B7-1 Antigen / Q000378:metabolism; D051940:B7-2 Antigen / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D053222:Fas Ligand Protein / Q000378:metabolism; D005260:Female; D006183:H-2 Antigens / Q000378:metabolism; D064532:Histocompatibility Antigen H-2D; D007371:Interferon-gamma / Q000378:metabolism; D037181:Lectins, C-Type / Q000378:metabolism; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology / Q000378:metabolism; D008546:Melanoma, Experimental / Q000188:drug therapy* / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D011134:Polysaccharides / Q000494:pharmacology*; D020940:Reishi / Q000737:chemistry*; D013154:Spleen / Q000166:cytology; D000099192:CD69 Antigens
D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D018122:B7-1 Antigen; D051940:B7-2 Antigen; D004365:Drugs, Chinese Herbal; D053222:Fas Ligand Protein; D006183:H-2 Antigens; D064532:Histocompatibility Antigen H-2D; D007371:Interferon-gamma; D037181:Lectins, C-Type; D011134:Polysaccharides; D000099192:CD69 Antigens; C497965:Cd86 protein, mouse; C505658:Fasl protein, mouse