In vivo kinetics and biodistribution of a Hantaan virus DNA vaccine after intramuscular injection in mice.
To study the kinetics in vivo of a Hantaan virus DNA vaccine, we constructed a fusion DNA vaccine, pEGFP/S, by cloning the S segment of Hantavirus into the vector, pEGFP-C1, which encodes Green fluorescent protein EGFP. In this report, we provide evidence that pEGFP/S was distributed and persistently expressed for more than 60 days in several organs after inoculation. Our findings suggest that the persistent immune responses induced by a Hantaan virus DNA vaccine are likely due to the plasmid pEGFP/S deposited in vivo, which acts as a booster immunization.
Wang
Si
S
Department of Medical microbiology and parasitology, Preclinical Medical College, China Medical University, Shenyang 110001, China.
Nie
Qing
Q
Zheng
Lan-yan
LY
Hu
Jun
J
Luo
En-jie
EJ
D000818:Animals; D005260:Female; D015870:Gene Expression; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D018063:Hantaan virus / Q000235:genetics*; D007273:Injections, Intramuscular; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids / Q000008:administration & dosage; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013194:Staining and Labeling / Q000379:methods; D013997:Time Factors; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000493:pharmacokinetics*; D014765:Viral Vaccines / Q000008:administration & dosage* / Q000493:pharmacokinetics*
D011994:Recombinant Proteins; D019444:Vaccines, DNA; D014765:Viral Vaccines; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins