Nectin 4 overexpression in ovarian cancer tissues and serum: potential role as a serum biomarker.
Early detection of ovarian cancer is difficult owing to the lack of specific and sensitive tests available. Previously, we found expression of nectin 4 to be increased in ovarian cancer compared with normal ovaries. Reverse transcriptase-polymerase chain reaction (RT-PCR) and quantitative RT-PCR validated the overexpression of nectin 4 messenger RNA in ovarian cancer compared with normal ovarian cell lines and tissues. Protein levels of nectin 4 were elevated in ovarian cancer cell lines and tissue compared with normal ovarian cell lines as demonstrated by Western immunoblotting, flow cytometry, and immunohistochemical staining of tissue microarray slides. Cleaved nectin 4 was detectable in a number of patient serum samples by enzyme-linked immunosorbent assay. In patients with benign gynecologic diseases with high serum CA125 levels, nectin 4 was not detected in the majority of cases, suggesting that nectin 4 may serve as a potential biomarker that helps discriminate benign gynecologic diseases from ovarian cancer in a panel with CA125.
Dept of Laboratory Medicine and Pathology, MMC 609, 420 Delaware St, SE, Minneapolis, MN 55455, USA.
Melissa S
MS
Derycke
Stefan E
SE
Pambuccian
C Blake
CB
Gilks
Steve E
SE
Kalloger
Abderrezak
A
Ghidouche
Marc
M
Lopez
Robin L
RL
Bliss
Melissa A
MA
Geller
Peter A
PA
Argenta
Katherine M
KM
Harrington
Amy P N
AP
Skubitz
D014408:Biomarkers, Tumor / Q000378:metabolism; D015153:Blotting, Western; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D045744:Cell Line, Tumor; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D010051:Ovarian Neoplasms / Q000378:metabolism*; D010053:Ovary / Q000378:metabolism*; D012372:ROC Curve; D020133:Reverse Transcriptase Polymerase Chain Reaction; D046888:Tissue Array Analysis; D000075983:Nectins
D014408:Biomarkers, Tumor; D015815:Cell Adhesion Molecules; C442228:NECTIN4 protein, human; D000075983:Nectins