High-sensitivity spectrofluorimetric determination of tiopronin based on inhibition of hemoglobin.
A highly sensitive and simple spectrofluorimetric method for the determination of tiopronin based on its inhibitory effect on the hemoglobin-catalyzed reaction of H(2)O(2) and L-tyrosine was developed. The concentration of tiopronin is linear with decreased fluorescence (ΔF) of the system under the optimal experimental conditions. The calibration graph is linear in the range 1.23 × 10(-8) to 3.06 × 10(-5) mol L(-1) with a detection limit of 6.13 × 10(-9) mol L(-1). The relative standard deviation was 4.38% for 11 determinations of 6.13 × 10(-6) mol L(-1). This method can be used for the determination of tiopronin in pharmaceuticals with satisfactory results.
Chen
Ya-Hong
YH
Department of Chemistry, Zhoukou Normal University, Zhoukou, 466000, People's Republic of China. chen-yh75@163.com
Tian
Feng-Shou
FS
Zhang
Gao-Feng
GF
D000818:Animals; D002384:Catalysis; D002417:Cattle; D006454:Hemoglobins / Q000037:antagonists & inhibitors*; D006861:Hydrogen Peroxide / Q000737:chemistry; D057230:Limit of Detection; D013050:Spectrometry, Fluorescence / Q000379:methods*; D008625:Tiopronin / Q000032:analysis*; D014443:Tyrosine / Q000737:chemistry
D006454:Hemoglobins; D014443:Tyrosine; D006861:Hydrogen Peroxide; D008625:Tiopronin