Determination of 3-hydroxy pterocarpan, a novel osteogenic compound in rat plasma by liquid chromatography-tandem mass spectrometry: application to pharmacokinetics study.
A rapid, sensitive and selective LC-MS/MS method for the quantitative analysis of 3-hydroxy pterocarpan (S006-1709) in female rat plasma has been developed and validated. A Discovery RP₁₈ column was used for the chromatographic elution using acetonitrile and 0.1% acetic acid in water as mobile phase (80:20 v/v) at the flow rate of 0.5 mL/min. MS/MS analysis was performed using a triple quadrupole mass spectrometer with electrospray ionization in negative ion mode using biochanin as an internal standard (IS). Extraction of S006-1709 and IS from rat plasma was done by liquid-liquid extraction method using diethyl ether. The LC-MS/MS method was sensitive with 1.95 ng/mL as the limit of detection and 3.9 ng/mL as the lower limit of quantification. The method was linear in the concentration range of 3.9-1000 ng/mL. The percentage bias for intraday and interday accuracy was not greater than 4.2 and the %RSD for intraday and interday precision was not greater than 13.2. The recoveries of S006-1709 and IS were 73.9-79.3 and 85.7%, respectively. S006-1709 was found to be stable in various stability studies. The validated LC-MS/MS method was successfully applied for the oral pharmacokinetics study of S006-1709 at 10 mg/kg in female Sprague-Dawley rats.
Pharmacokinetics and Metabolism Division, Central Drug Research Institute, Lucknow-226001, Uttar Pradesh, India.
Lakshmi
L
Manickavasagam
Sonal
S
Gupta
Smriti
S
Mishra
Amit
A
Kumar
Ashutosh
A
Raghuvanshi
Atul
A
Goel
Divya
D
Singh
Girish Kumar
GK
Jain
D000284:Administration, Oral; D000818:Animals; D005591:Chemical Fractionation; D002853:Chromatography, Liquid / Q000379:methods*; D004355:Drug Stability; D004967:Estrogens / Q000032:analysis / Q000493:pharmacokinetics; D005260:Female; D007529:Isoflavones / Q000032:analysis* / Q000493:pharmacokinetics; D016014:Linear Models; D036343:Pterocarpans / Q000032:analysis* / Q000493:pharmacokinetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
D004967:Estrogens; D007529:Isoflavones; D036343:Pterocarpans