Potential role of viral surface glycoproteins in the replication of H3N2 triple reassortant influenza A viruses in swine and turkeys.
The H3N2 triple reassortant (TR) influenza viruses emerged in swine in 1998 and then in turkeys in 2003. It was then hypothesized that these viruses crossed the species barrier and transmitted from pigs to turkeys. In previous work we identified viruses with different transmission behavior between the two species, of which A/turkey/Ohio/313053/04 (TK04) transmitted both ways between swine and turkeys, and A/swine/North Carolina/03 (SW03) did not transmit either way between the two species. Utilizing the 12-plasmid reverse genetics (RG) system, we rescued two viruses (TK04 and SW03) with potentially different transmission behavior between pigs and turkeys. Single gene reassortants (SGR) were generated by switching the hemagglutinin (HA) or the neuraminidase (NA) genes between both viruses, and were evaluated for replication in vitro (pig and turkey tracheal/bronchial epithelial cells) and in vivo (pigs and turkeys). RG-created TK04 replicated more efficiently than SW03 in vitro and in vivo. Additionally, TK04 exhibited better binding affinity to plasma membrane preparations (PMP) from pig and turkey tracheal/bronchial epithelial cells compared to SW03. In study with SGR viruses, the HA protein was found to be essential for TK04 virus transmission amongst turkeys, but not sole factor contributing to the efficient replication of virus in turkeys and pigs. Such findings further highlight the polygenic nature of influenza virus pathogenesis.
Yassine
Hadi M
HM
Food Animal Health Research Program, Ohio Agricultural Research and Development Center, The Ohio State University, 1680 Madison Ave., Wooster, OH 44691, USA.
Khatri
Mahesh
M
Lee
Chang-Won
CW
Saif
Yehia M
YM
D000818:Animals; D004187:Disaccharides; D004573:Electrolytes; D004847:Epithelial Cells / Q000821:virology; D005971:Glutamates; D005978:Glutathione; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics*; D006639:Histidine; D053122:Influenza A Virus, H3N2 Subtype / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology*; D005585:Influenza in Birds / Q000635:transmission / Q000821:virology; D008353:Mannitol; D009439:Neuraminidase / Q000235:genetics; D009976:Orthomyxoviridae Infections / Q000635:transmission / Q000821:virology; D012367:RNA, Viral / Q000235:genetics; D016865:Reassortant Viruses / Q000235:genetics*; D013552:Swine / Q000821:virology*; D013553:Swine Diseases / Q000635:transmission / Q000821:virology; D014422:Turkeys / Q000821:virology*; D014779:Virus Replication
C088453:Celsior; D004187:Disaccharides; D004573:Electrolytes; D005971:Glutamates; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D012367:RNA, Viral; C483468:hemagglutinin, human influenza A virus; D008353:Mannitol; D006639:Histidine; D009439:Neuraminidase; D005978:Glutathione