Glycosylation of mucins present in gastric juice: the effect of helicobacter pylori eradication treatment.
It is suggested that gastric mucins, and in particular some specific glycan structures that can act as carbohydrate receptors, are involved in the interactions with Helicobacter pylori adhesins. The main aim of our study was to evaluate glycosylation pattern of glycoproteins of gastric juice before and at the end of eradication therapy. Gastric juices were taken from 13 clinical patients and subjected to analysis. Pooled fractions of the void volume obtained after gel filtration were subjected to ELISA tests. To assess the relative amounts of carbohydrate structures, lectins and monoclonal antibodies were used. Changes in the level of MUC 1 and MUC 5AC mucins and of carbohydrate structures, which are suggested to be receptors for Helicobacter pylori adhesins, were observed by the end of the eradication treatment. Our results support the idea about the involvement of MUC 5AC and MUC 1 with some specific sugar structures in the mechanism of Helicobacter pylori infection.
Department of Medical Chemistry, Medical University of Białystok, Poland. iwona@umwb.edu.pl
Iwona
I
Radziejewska
Małgorzata
M
Borzym-Kluczyk
Zbigniew
Z
Namiot
Ewa
E
Stefańska
D000328:Adult; D002241:Carbohydrates / Q000032:analysis*; D002850:Chromatography, Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005750:Gastric Juice / Q000737:chemistry*; D006031:Glycosylation; D016481:Helicobacter Infections / Q000188:drug therapy; D016480:Helicobacter pylori / Q000302:isolation & purification; D006801:Humans; D008297:Male; D008875:Middle Aged; D055271:Mucin 5AC / Q000032:analysis*; D018396:Mucin-1 / Q000032:analysis*
D002241:Carbohydrates; C060192:MUC1 protein, human; C524678:MUC5AC protein, human; D055271:Mucin 5AC; D018396:Mucin-1