The pharmacological effects of morroniside and loganin isolated from Liuweidihuang Wan, on MC3T3-E1 cells.
Liuweidihuang wan (LW), initially a well-known formula for curing "wu chi wu ruan", is commonly used nowadays for clinical treatment of postmenopausal osteoporosis (PO), but the identity of the effective substance(s) remains unclear. The present study was designed to evaluate the effects of morroniside and loganin isolated from LW on the proliferation, differentiation and apoptosis of MC3T3-E1 cells, as well as the possible mechanism of action. Morroniside and loganin had no effects on the proliferation of MC3T3-E1 cells, but both susbtances could improve the activity of alkaline phosphatase (ALP), and increase the contents of collagen type I and osteocalcin. Simultaneously, the mRNA expression of caspase-3, capase-9, RANKL was down-regulated and that of bcl-2 was up-regulated, which partially explains the anti-osteoporosis mechanism in MC3T3-E1 cells. In conclusion, morroniside and loganin may directly promote the differentiation and inhibit the apoptosis of MC3T3-E1 cells, and accordingly indirectly reduce bone resorption, which makes them promising natural drugs leads for treating PO in the near future.
Li
Manyu
M
National TCM Key Lab of Serum Pharmacochemistry, Heilongjiang University of Chinese Medicine, Heping Road 24, Harbin 150040, China.
Wang
Wei
W
Wang
Ping
P
Yang
Kun
K
Sun
Hui
H
Wang
Xijun
X
D016475:3T3 Cells / Q000187:drug effects*; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000627:therapeutic use; D005260:Female; D006027:Glycosides / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D039823:Iridoids / Q000737:chemistry / Q000494:pharmacology*; D008297:Male; D051379:Mice; D015663:Osteoporosis, Postmenopausal / Q000188:drug therapy
D004365:Drugs, Chinese Herbal; D006027:Glycosides; D039823:Iridoids; C476907:liu-wei-di-huang wan; C488401:morroniside; C059516:loganin