Leukocyte and platelet changes following low-dose lipopolysaccharide administration in five dogs.
Effects of low-dose LPS (0.1 μg/kg i.v.) on leukocyte and platelet parameters measured using an Advia 120 hematology analyzer were investigated. Five dogs received a saline sham treatment prior to LPS, and blood was collected before and 3, 6, and 24 h post-treatment. LPS-treated dogs had mild neutrophil toxic change and increased neutrophil bands at 3 and 6 h. Compared to saline-treated controls, total leukocyte, neutrophil, and monocyte counts of LPS-treated dogs were significantly decreased at 3 h and increased at 24 h. Compared to baseline, total leukocyte counts of LPS-treated dogs were significantly decreased at 3 h and increased at 24 h. Mean platelet volume was significantly increased and mean platelet component concentration was decreased at 3 h compared to baseline. Platelet count was significantly decreased at 3 and 6 h; plateletcrit did not change significantly. High dosage is not required in order to detect LPS-mediated hematologic effects in dogs. Low-dose LPS administration causes significant changes in leukocyte and platelet indices in dogs without causing severe clinical signs or death.
Flatland
B
B
Department of Pathobiology, College of Veterinary Medicine, University of Tennessee, Knoxville, TN 379996, United States. bflatlan@utk.edu
Fry
M M
MM
LeBlanc
C J
CJ
Rohrbach
B W
BW
D000818:Animals; D001792:Blood Platelets / Q000187:drug effects*; D004283:Dog Diseases / Q000139:chemically induced* / Q000378:metabolism; D004285:Dogs; D007962:Leukocytes / Q000187:drug effects*; D008070:Lipopolysaccharides / Q000633:toxicity*; D013997:Time Factors
D008070:Lipopolysaccharides