Synthesis and characterization of an exopolysaccharide by antarctic yeast strain Cryptococcus laurentii AL₁₀₀.
An exopolysaccharide-producing Antarctic yeast strain was selected and identified as Cryptococcus laurentii AL₁₀₀. The physiological properties of the strain and its ability to utilize and biotransform different carbon sources (pentoses, hexoses, and oligosaccharides) into exopolysaccharide and biomass were investigated. Sucrose was chosen as a suitable and accessible carbon source. The biosynthetic capacity of the strain was studied in its dynamics at different sucrose concentrations (20, 30, 40, and 50 g/L) and temperatures (22 and 24 °C). The maximum biopolymer quantity of 6.4 g/L was obtained at 40 g/L of sucrose, 22 °C temperature and 96-h fermentation duration. The newly synthesized microbial carbohydrate was a heteropolysaccharide having the following monosaccharide composition: arabinose, 61.1%; mannose, 15.0%; glucose, 12.0%; galactose, 5.9%; and rhamnose, 2.8%. It was characterized by polydispersity of the polymer molecule, 60% of it having molecular mass of 4200 Da. The exopolysaccharide demonstrated good emulsifying and stabilizing properties with regard to oil/water emulsions and a pronounced synergistic effect with other hydrocolloids such as xanthan gum, guar gum, and alginate.
Pavlova
Kostantsa
K
Laboratory of Applied Biotechnologies, Institute of Microbiology, Bulgarian Academy of Sciences, 139 Ruski Blvd, Plovdiv 4000, Bulgaria.
Rusinova-Videva
Snezhana
S
Kuncheva
Margarita
M
Kratchanova
Maria
M
Gocheva
Mariana
M
Dimitrova
Stela
S
D002240:Carbohydrate Sequence; D003454:Cryptococcus / Q000737:chemistry / Q000378:metabolism*; D005110:Extracellular Space / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000096:biosynthesis* / Q000737:chemistry*; D013395:Sucrose / Q000378:metabolism
D011134:Polysaccharides; D013395:Sucrose