Energy transfer between surface-immobilized light-harvesting chlorophyll a/b complex (LHCII) studied by surface plasmon field-enhanced fluorescence spectroscopy (SPFS).
The major light-harvesting chlorophyll a/b complex (LHCII) of the photosynthetic apparatus in green plants can be viewed as a protein scaffold binding and positioning a large number of pigment molecules that combines rapid and efficient excitation energy transfer with effective protection of its pigments from photobleaching. These properties make LHCII potentially interesting as a light harvester (or a model thereof) in photoelectronic applications. Most of such applications would require the LHCII to be immobilized on a solid surface. In a previous study we showed the immobilization of recombinant LHCII on functionalized gold surfaces via a 6-histidine tag (His tag) in the protein moiety. In this work the occurrence and efficiency of Förster energy transfer between immobilized LHCII on a functionalized surface have been analyzed by surface plasmon field-enhanced fluorescence spectroscopy (SPFS). A near-infrared dye was attached to some but not all of the LHC complexes, serving as an energy acceptor to chlorophylls. Analysis of the energy transfer from chlorophylls to this acceptor dye yielded information about the extent of intercomplex energy transfer between immobilized LHCII.
Institut für Allgemeine Botanik der Johannes-Gutenberg Universität, Müllerweg 6, 55099 Mainz, Germany.
Rolf
R
Lauterbach
Jing
J
Liu
Wolfgang
W
Knoll
Harald
H
Paulsen
D000042:Absorption; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism*; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry; D007700:Kinetics; D045342:Light-Harvesting Protein Complexes / Q000737:chemistry* / Q000378:metabolism*; D008958:Models, Molecular; D018532:Pisum sativum / Q000201:enzymology; D011487:Protein Conformation; D013499:Surface Properties
D004800:Enzymes, Immobilized; D005456:Fluorescent Dyes; D045342:Light-Harvesting Protein Complexes