Human, mouse or rat? Species authentication of glioma-derived cell cultures.
Cell culture and the use of cell lines are often fundamental requirements in basic scientific research. It is of the utmost importance for researchers to ensure that the cell lines in use have a well defined origin and are routinely re-analysed to highlight possible areas of contamination. In this preliminary study species specific primers were designed to easily distinguish between human, mouse and rat DNA with standard agarose gel electrophoresis. Inter-species contamination is often the most common form of contamination experienced, with the most common of cell lines in use being of human, mouse and rat derivation. A PCR-based assay was therefore developed to ensure an accurate, quick and cost effective means of determining any cell line contamination which could be easily executed on a routine basis. Furthermore, this simple PCR is able to identify the species in the inter-species mixture of DNA and therefore provides a valuable tool for the authentication of human cell lines.
Cellular & Molecular Neuro-oncology Group, Cellular and Molecular Medicine Research Division, Institute of Biomedical & Biomolecular Sciences, School of Pharmacy & Biomedical Sciences, White Swan Road, University of Portsmouth, Portsmouth, UK.
Samantha C
SC
Higgins
Hedda
H
Steingrimsdottir
Geoffrey J
GJ
Pilkington
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D001932:Brain Neoplasms / Q000473:pathology*; D045744:Cell Line, Tumor; D002847:Chromatography, Agarose; D017931:DNA Primers / Q000235:genetics; D004273:DNA, Neoplasm / Q000235:genetics; D005910:Glioma / Q000473:pathology*; D006801:Humans; D007120:Immunochemistry; D016256:Lewis X Antigen / Q000276:immunology; D051379:Mice; D051381:Rats; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013045:Species Specificity
D000911:Antibodies, Monoclonal; D017931:DNA Primers; D004273:DNA, Neoplasm; D016256:Lewis X Antigen; D011933:Reagent Kits, Diagnostic