Measurement of thioredoxin and thioredoxin reductase.
The thioredoxin system is ubiquitous, providing reducing equivalents to essential biosynthetic enzymes like ribonucleotide reductase. It is essential for cellular redox regulation, control of oxidative stress, and protection against oxidative damage. This unit includes protocols for measuring thioredoxin or thioredoxin reductase in biological preparations or as purified enzymes.
Arnér
E S
ES
Karolinska Institutet, Stockholm, Sweden.
Holmgren
A
A
D000818:Animals; D004229:Dithiothreitol / Q000737:chemistry; D006801:Humans; D007328:Insulin / Q000737:chemistry; D007700:Kinetics; D010084:Oxidation-Reduction; D013880:Thioredoxin-Disulfide Reductase / Q000737:chemistry*; D013879:Thioredoxins / Q000737:chemistry*
D007328:Insulin; D013879:Thioredoxins; D013880:Thioredoxin-Disulfide Reductase; D004229:Dithiothreitol