Mass mortality associated with koi herpesvirus in wild common carp in Canada.
Koi herpesvirus (KHV) was identified as being associated with more than one mortality event affecting common carp in Canada. The first was an extensive mortality event that occurred in 2007 in the Kawartha Lakes region, Ontario, affecting Lakes Scugog and Pigeon. Fish had branchial necrosis and hepatic vasculitis with an equivocal interstitial nephritis. Several fish also had branchial columnaris. Subsequent mortality events occurred in 2008 in additional bodies of water in south-central Ontario, such as Lake Katchewanooka and outside of Ontario in Lake Manitoba, Manitoba. Koi herpesvirus was detected in fish submitted for examination from all of these lakes by polymerase chain reaction (PCR), and sequence of the PCR product revealed 100% homology to KHV strains U and I. Real-time PCR analysis of KHV-infected wild carp revealed viral loads ranging from 6.02×10(1) to 2.4×10(6) copies μg(-1) host DNA. This is the first report of KHV in Canada.
Fisheries & Oceans Canada, Pacific Biological Station, 3190 Hammond Bay Road, Nanaimo, British Colombia V9T 6N7, Canada. kyle.garver@dfo-mpo.gc.ca
Kyle A
KA
Garver
Lowia
L
Al-Hussinee
Laura M
LM
Hawley
Tamara
T
Schroeder
Sandra
S
Edes
Veronique
V
LePage
Elena
E
Contador
Spencer
S
Russell
Stephen
S
Lord
Roselynn M W
RM
Stevenson
Brian
B
Souter
Elizabeth
E
Wright
John S
JS
Lumsden
D000818:Animals; D002170:Canada / Q000453:epidemiology; D002347:Carps / Q000821:virology*; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D005393:Fish Diseases / Q000453:epidemiology / Q000401:mortality*; D006564:Herpesviridae / Q000235:genetics / Q000302:isolation & purification; D006566:Herpesviridae Infections / Q000453:epidemiology / Q000401:mortality / Q000662:veterinary*; D008297:Male; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D019562:Viral Load / Q000662:veterinary
D004279:DNA, Viral