Olfactory ensheathing cells promote neurite outgrowth from co-cultured brain stem slice.
Cell therapy aiming at the replacement of degenerated neurons is a very attractive approach. By using an established in vitro organotypic brain stem (BS) slice culture we screen for candidate donor cells, some of them being further functionally assessed in in vivo models of sensorineural hearing loss. Both in vitro and in vivo systems show that implanted cells face challenges of survival, targeted migration, differentiation and functional integration with the host tissue. Low success rates are possibly due to the lack of necessary neurotrophic factors, adhesion molecules and guiding cues. Olfactory ensheathing cells (OECs) have been shown to express a number of neurotrophic factors and to promote axonal growth through cell to cell interactions. In the present study we co-cultured OECs with organotypic BS slice in order to see if OECs can serve as a facilitator when screening candidate donor cells in an organotypic culture setup. Here we show that OECs when co-cultured with the auditory BS slice not only promote neurite outgrowth from the cochlear nucleus (CN) region of the BS slice but also support cells by having BS slice axons growing along their processes. These findings further suggest that OECs may enhance survival and targeted migration of candidate donor cells suitable for cell therapy in vitro and in vivo. This article is part of a Special Issue entitled: Understanding olfactory ensheathing glia and their prospect for nervous system repair.
Center for Hearing and Communication Research, Karolinska University Hospital, Stockholm, Sweden. yu.jiao@ki.se
Yu
Y
Jiao
Ekaterina
E
Novozhilova
Alexandra
A
Karlén
Jonas
J
Muhr
Petri
P
Olivius
D000818:Animals; D001933:Brain Stem / Q000166:cytology* / Q000502:physiology*; D002470:Cell Survival / Q000502:physiology; D018920:Coculture Techniques / Q000379:methods; D005260:Female; D016501:Neurites / Q000502:physiology*; D009830:Olfactory Bulb / Q000166:cytology* / Q000502:physiology*; D009924:Organ Culture Techniques; D051381:Rats; D017207:Rats, Sprague-Dawley