[Construction and application of fluorescence labeled multiplex typing system for 3 new miniSTR loci].
OBJECTIVE
To establish a miniSTR multiplex set including three STR loci unlinked from the CODIS loci: D1S1676, D6S1274 and D17S1299, to generate amplified fragment less than 115 bp in size and to study the genotype of degraded DNA samples.


METHODS
After amplification with different fluorescence labeled primers, the amplified products from 100 unrelated individual and 2 highly degraded specimens were analyzed by 310 Genetic Analyzer.


RESULTS
Three miniSTR loci were determined by fluorescence-labeled multiplex-PCR technique. Each locus was successfully genotyped in all 100 samples. In D1S1676, D6S1274 and D17S1299 loci, 9, 9, 7 alleles and 27, 23, 18 genotypes were observed respectively. The distribution of genotype for three miniSTR loci in Chengdu Han population was in accordance with Hardy-Weinberg equilibrium. The combined exclusion probability and the combined discrimination power of the three STR loci in Chengdu Han population were 0.9991 and 0.9160 respectively.


CONCLUSIONS
This miniSTR multiplex set could be used in individual identification and paternity test. It also provides a new method in the analysis of degraded DNA sample.
Department of Forensic Science, North Sichuan Medical College, Nanchong 637000, China. dbing04@163.com
Bing
B
Du
Ji-ping
JP
Jiang
Hong
H
Du
Lin
L
Zhang
D000483:Alleles; D002681:China / Q000208:ethnology; D004247:DNA / Q000032:analysis* / Q000378:metabolism; D016172:DNA Fingerprinting / Q000379:methods*; D017931:DNA Primers; D053803:Forensic Genetics / Q000379:methods; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D011110:Polymorphism, Genetic; D020080:Tandem Repeat Sequences
D017931:DNA Primers; D004247:DNA