Proteomic analysis of bovine nucleolus.
Nucleolus is the most prominent subnuclear structure, which performs a wide variety of functions in the eukaryotic cellular processes. In order to understand the structural and functional role of the nucleoli in bovine cells, we analyzed the proteomic composition of the bovine nucleoli. The nucleoli were isolated from Madin Darby bovine kidney cells and subjected to proteomic analysis by LC-MS/MS after fractionation by SDS-PAGE and strong cation exchange chromatography. Analysis of the data using the Mascot database search and the GPM database search identified 311 proteins in the bovine nucleoli, which contained 22 proteins previously not identified in the proteomic analysis of human nucleoli. Analysis of the identified proteins using the GoMiner software suggested that the bovine nucleoli contained proteins involved in ribosomal biogenesis, cell cycle control, transcriptional, translational and post-translational regulation, transport, and structural organization.
Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, Canada.
Amrutlal K
AK
Patel
Doug
D
Olson
Suresh K
SK
Tikoo
D000818:Animals; D002417:Cattle; D002466:Cell Nucleolus / Q000378:metabolism*; D002522:Chlorocebus aethiops; D002853:Chromatography, Liquid / Q000379:methods; D004396:Coloring Agents / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D006801:Humans; D008164:Luminescent Proteins / Q000494:pharmacology; D013058:Mass Spectrometry / Q000379:methods; D020543:Proteome; D040901:Proteomics / Q000379:methods*; D012984:Software; D013347:Subcellular Fractions / Q000378:metabolism; D014709:Vero Cells
D004396:Coloring Agents; D008164:Luminescent Proteins; D020543:Proteome; C413663:fluorescent protein 583