Separation and identification of heterocyclic nitrogen compounds in biooil derived by fast pyrolysis of chicken manure.
N-heterocyclics were separated from a biooil, generated by the pyrolysis of chicken manures by column chromatography over neutral alumina and silica, and identified by Pyrolysis Field Ionization Mass Spectrometry (Py-FIMS) and Electrospray Ionization Mass Spectrometry (ESI-MS). Identities of chemical structures, whose presence was indicated by ESI-MS, were confirmed by comparing the Collision-Induced Dissociations (CID's) mass spectra of unknown and standards. The following seven base structures were identified: pyrazine, benzoquinoline, carbazole, phenylpyridine, indole, pyrazole and pyridine. Available hydrogens bonded to ring carbons and nitrogens on the seven N-heterocyclics were increasingly substituted by alkyl groups, mainly methylene groups (m/z 14) to yield mono-, di-, tri- methyl N-heterocyclics. In some instances, longer alkyl chains, such as ethyl, propyl, up to heptyl groups were the substituents.
Eastern Cereal and Oilseed Research Center, Agriculture and Agri-Food Canada, Ottawa, Canada.
Zakir H
ZH
Kazi
Morris I
MI
Schnitzer
Carlos M
CM
Monreal
Paul
P
Mayer
D000818:Animals; D056804:Biofuels / Q000032:analysis*; D002645:Chickens; D006571:Heterocyclic Compounds / Q000737:chemistry* / Q000302:isolation & purification*; D008372:Manure / Q000032:analysis*; D015394:Molecular Structure; D017672:Nitrogen Compounds / Q000737:chemistry* / Q000302:isolation & purification*; D021241:Spectrometry, Mass, Electrospray Ionization
D056804:Biofuels; D006571:Heterocyclic Compounds; D008372:Manure; D017672:Nitrogen Compounds