Basal bioenergetic abnormalities in skeletal muscle from ryanodine receptor malignant hyperthermia-susceptible R163C knock-in mice.
Malignant hyperthermia (MH) and central core disease in humans have been associated with mutations in the skeletal ryanodine receptor (RyR1). Heterozygous mice expressing the human MH/central core disease RyR1 R163C mutation exhibit MH when exposed to halothane or heat stress. Considering that many MH symptoms resemble those that could ensue from a mitochondrial dysfunction (e.g. metabolic acidosis and hyperthermia) and that MH-susceptible mice or humans have a higher than normal cytoplasmic Ca(2+) concentration at rest, we evaluated the role of mitochondria in skeletal muscle from R163C compared with wild type mice under basal (untriggered) conditions. R163C skeletal muscle exhibited a significant increase in matrix Ca(2+), increased reactive oxygen species production, lower expression of mitochondrial proteins, and higher mtDNA copy number. These changes, in conjunction with lower myoglobin and glycogen contents, Myh4 and GAPDH transcript levels, GAPDH activity, and lower glucose utilization suggested a switch to a compromised bioenergetic state characterized by both low oxidative phosphorylation and glycolysis. The shift in bioenergetic state was accompanied by a dysregulation of Ca(2+)-responsive signaling pathways regulated by calcineurin and ERK1/2. Chronically elevated resting Ca(2+) in R163C skeletal muscle elicited the maintenance of a fast-twitch fiber program and the development of insulin resistance-like phenotype as part of a metabolic adaptation to the R163C RyR1 mutation.
Giulivi
Cecilia
C
Department of Molecular Biosciences, School of Veterinary Medicine, University of California, Davis, California 95616, USA. cgiulivi@ucdavis.edu
Ross-Inta
Catherine
C
Omanska-Klusek
Alicja
A
Napoli
Eleonora
E
Sakaguchi
Danielle
D
Barrientos
Genaro
G
Allen
Paul D
PD
Pessah
Isaac N
IN
D000818:Animals; D001481:Basal Metabolism / Q000235:genetics*; D019703:Calcineurin / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D003964:Diaphragm / Q000378:metabolism / Q000503:physiopathology; D005260:Female; D005786:Gene Expression Regulation; D055879:Gene Knock-In Techniques; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D006801:Humans; D007700:Kinetics; D008305:Malignant Hyperthermia / Q000235:genetics / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D051379:Mice; D008928:Mitochondria / Q000378:metabolism; D018482:Muscle, Skeletal / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D009154:Mutation; D018384:Oxidative Stress / Q000235:genetics; D010100:Oxygen / Q000378:metabolism; D010539:Permeability; D051381:Rats; D019837:Ryanodine Receptor Calcium Release Channel / Q000235:genetics*; D015398:Signal Transduction / Q000235:genetics
D019837:Ryanodine Receptor Calcium Release Channel; D019703:Calcineurin; D010100:Oxygen; D002118:Calcium