Evaluation of Plasmodium vivax ELISA for the blood screen.
Plasmodium vivax malaria is the indigenous strain in the Republic of Korea (ROK). Plasmodium vivax can be transmitted through the transfusions of various blood components, which became a severe problem with the safety of blood transfusions and blood-related products in ROK. We evaluated a P. vivax-specific enzyme-linked immunosorbent assay (Genedia Malaria Ab ELISA 2.0, Green Cross, ROK) with blood samples from four groups: 251 samples from P. vivax-infected patients, 39 samples from post-treatment patients upon follow-up, 200 samples from healthy volunteers and 421 samples from domestic travellers to and from high endemic areas of ROK. The positive cases from the ELISA test were confirmed by both Giemsa microscopic and polymerase chain reaction methods. The clinical sensitivity and specificity of detecting P. vivax with ELISA test were 94.4% and 99.0%, respectively. Thirteen of 421 domestic travellers (3.0%) to endemic areas tested positive. The results indicate the effectiveness of detecting antibodies against P. vivax in blood with Genedia Malaria Ab ELISA 2.0 test in a large blood screen setting.
Nam
Myung-Hyun
MH
Department of Laboratory Medicine, College of Medicine, Korea University, Seoul, Korea.
Kim
Jang Su
JS
Cho
Chi Hyun
CH
Han
Eun Taek
ET
Lee
Won Ja
WJ
Lee
Hee Kyung
HK
An
Seong Soo A
SS
Lim
Chae Seung
CS
Lee
Kap No
KN
D000913:Antibodies, Protozoan / Q000097:blood*; D001782:Blood Donors; D017848:Blood-Borne Pathogens / Q000302:isolation & purification; D019353:Endemic Diseases; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D016780:Malaria, Vivax / Q000175:diagnosis* / Q000453:epidemiology / Q000635:transmission; D008403:Mass Screening / Q000379:methods; D018512:Parasitemia / Q000175:diagnosis; D010966:Plasmodium vivax / Q000276:immunology*; D056910:Republic of Korea / Q000453:epidemiology; D012680:Sensitivity and Specificity
D000913:Antibodies, Protozoan