Ionic-liquid-based catch and release mass spectroscopy tags for enzyme monitoring.
A novel, inexpensive and versatile ionic-liquid-based catch and release mass spectrometry tag (I-Tag) that facilitates substrate purification, fast, robust and sensitive enzymatic reaction monitoring and quantitative kinetic analysis has been developed. The applicability of the system has been demonstrated in an enzymatic assay with β-1,4-galactosyltransferase.
School of Chemistry, University of Bristol, Cantock's Close, Bristol, BS8 1TS, UK. M.C.Galan@bristol.ac.uk
M Carmen
MC
Galan
Anh Tuan
AT
Tran
Claire
C
Bernard
D002853:Chromatography, Liquid; D052578:Ionic Liquids / Q000737:chemistry*; D007700:Kinetics; D013058:Mass Spectrometry; D009237:N-Acetyllactosamine Synthase / Q000737:chemistry* / Q000378:metabolism
D052578:Ionic Liquids; D009237:N-Acetyllactosamine Synthase