Biomolecule-stabilized Au nanoclusters as a fluorescence probe for sensitive detection of glucose.
In this work, biomolecule-stabilized Au nanoclusters were demonstrated as a novel fluorescence probe for sensitive and selective detection of glucose. The fluorescence of Au nanoclusters was found to be quenched effectively by the enzymatically generated hydrogen peroxide (H(2)O(2)). By virtue of the specific response, the present assay allowed for the selective determination of glucose in the range of 1.0×10(-5) M to 0.5×10(-3) M with a detection limit of 5.0×10(-6) M. The absorption spectroscopy, X-ray photoelectron spectroscopy (XPS) and fluorescence decay studies were then performed to discuss the quenching mechanism. In addition, we demonstrated the application of the present approach in real serum samples, which suggested its great potential for diagnostic purposes.
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Graduate School of the Chinese Academy of Sciences, Renming Street 5625, Changchun 130022, China.
Lihua
L
Jin
Li
L
Shang
Shaojun
S
Guo
Youxing
Y
Fang
Dan
D
Wen
Li
L
Wang
Jianyuan
J
Yin
Shaojun
S
Dong
D015374:Biosensing Techniques / Q000295:instrumentation*; D004795:Enzyme Stability; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005456:Fluorescent Dyes / Q000032:analysis / Q000737:chemistry*; D005947:Glucose / Q000032:analysis / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D013050:Spectrometry, Fluorescence / Q000295:instrumentation*
D005456:Fluorescent Dyes; D006046:Gold; D006861:Hydrogen Peroxide; D005947:Glucose