Proteomic analysis of plasma proteins in Japanese semisuper centenarians.
We performed proteomic analysis of plasma proteins in Japanese semisuper centenarians (SSCs) (>105 years) and young controls (20-39 years), and found that 18 protein spots were altered in the plasma of SSCs. From peptide mass fingerprinting following in-gel digestion, it was demonstrated that paraoxonase/arylesterase 1 (PON 1) and apolipoprotein E were decreased, while haptoglobin β-chain, α(1)-microglobulin, and clusterin precursor were increased in SSCs. Interestingly, proteins related to oxidative stress, PON1, haptoglobin, α(1)-microglobulin, and clusterin, were altered in SSCs. These results suggest that systemic redox regulation is important for the longevity of SSCs. Overall, proteomics analysis is a powerful technique to search for useful biomarkers for future studies in gerontology and to characterize the individual proteins associated with successful aging of SSCs.
Miura
Yuri
Y
Research Team for Mechanism of Aging, Tokyo Metropolitan Institute of Gerontology, Tokyo 173-0015, Japan.
Sato
Yuji
Y
Arai
Yasumichi
Y
Abe
Yukiko
Y
Takayama
Michiyo
M
Toda
Tosifusa
T
Hirose
Nobuyoshi
N
Endo
Tamao
T
D000369:Aged, 80 and over; D000375:Aging / Q000378:metabolism*; D000510:Alpha-Globulins / Q000378:metabolism; D001057:Apolipoproteins E / Q000378:metabolism; D043303:Aryldialkylphosphatase / Q000378:metabolism; D044466:Asian People; D015415:Biomarkers / Q000097:blood; D001798:Blood Proteins / Q000378:metabolism*; D051152:Clusterin / Q000378:metabolism; D005260:Female; D006242:Haptoglobins / Q000378:metabolism; D006801:Humans; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000502:physiology; D010449:Peptide Mapping; D040901:Proteomics
D000510:Alpha-Globulins; D001057:Apolipoproteins E; D015415:Biomarkers; D001798:Blood Proteins; C495242:CLU protein, human; D051152:Clusterin; C485359:HP protein, human; D006242:Haptoglobins; C016001:alpha-1-microglobulin; D043303:Aryldialkylphosphatase; C468406:PON1 protein, human