Novel indole C-glycosides from Isatis indigotica and their potential cytotoxic activity.
Two novel indole C-glycosides, which were the first reported alkaloids C-glycosides from Isatis indigotica, together with five known alkaloids were isolated. The novel alkaloids were elucidated to be indole-3-acetonitrile-4-methoxy-2-C-β-D-glucopyranoside (1) and N-methoxy-indole-3-acetonitrile-2-C-β-D-glucopyranoside (2) on the basis of spectroscopic analysis. 1 exhibited significant cytotoxic activities against human myeloid leukemia HL-60 and human liver cancer HepG2 cells with the IC(50) of 1.3 ± 0.1 and 2.1 ± 0.3 μM, respectively. 2 showed potential cytotoxic activities against HL-60 and human myeloid leukemia Mata cells with the IC(50) of 5.1 ± 0.4 and 12.1 ± 0.8 μM, respectively.
College of Pharmacy, Wuhan University, Wuhan, 430071, China.
Yixuan
Y
Wu
Zhen-Xue
ZX
Zhang
Hui
H
Hu
Dongmei
D
Li
Guofu
G
Qiu
Xianming
X
Hu
Xiangjiu
X
He
D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D045744:Cell Line, Tumor; D005960:Glucosides; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D006801:Humans; D007211:Indoles / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D031219:Isatis / Q000737:chemistry*; D007938:Leukemia / Q000188:drug therapy*; D008113:Liver Neoplasms / Q000188:drug therapy*; D015394:Molecular Structure; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D018517:Plant Roots
D000972:Antineoplastic Agents, Phytogenic; D005960:Glucosides; D006027:Glycosides; D007211:Indoles; C560473:N-methoxy-indole-3-acetonitrile-2-C-beta-D-glucopyranoside; D010936:Plant Extracts; C560474:indole-3-acetonitrile-4-methoxy-2-C-beta-D-glucopyranoside