A simple and rapid GC/MS method for the simultaneous determination of gaseous metabolites.
We modified and tuned a commercial model of a gas chromatography/mass spectrometry (GC/MS) instrument to develop a simple and rapid method for the simultaneous quantification of a variety of gas species. Using the developed method with the newly modified instrument, gas species such as H(2), N(2), O(2), CO, NO, CH(4), CO(2), and N(2)O, which are common components of microbial metabolism, were accurately identified based on their retention times and/or mass-to-charge ratios (m/z) in less than 2.5 min. By examining the sensitivities and dynamic ranges for the detection of H(2), N(2), O(2), CH(4), CO(2), and N(2)O, it was demonstrated that the method developed in this study was sufficient for accurately monitoring the production and the consumption of these gaseous species during microbial metabolism. The utility of the new method was demonstrated by a denitrification study with Pseudomonas aureofaciens ATCC 13985(T). This method will be suitable for a variety of applications requiring the identification of gaseous metabolites in microorganisms, microbial communities, and natural ecosystems.
Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan.
Kazuo
K
Isobe
Keisuke
K
Koba
Shingo
S
Ueda
Keishi
K
Senoo
Shigeaki
S
Harayama
Yuichi
Y
Suwa
D058440:Denitrification; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D005740:Gases / Q000737:chemistry* / Q000378:metabolism*; D055432:Metabolomics / Q000379:methods*; D011549:Pseudomonas / Q000737:chemistry* / Q000378:metabolism*; D012680:Sensitivity and Specificity
D005740:Gases