Electrochemical biosensors based on redox carbon nanotubes prepared by noncovalent functionalization with 1,10-phenanthroline-5,6-dione.
To improve the electrocatalytic activities of carbon nanotubes (CNT) towards the oxidation of nicotinamide adenine dinucleotide (NADH), we derive them with a redox mediator, 1,10-phenanthroline-5,6-dione (PD), by the noncovalent functionalization method. The redox carbon nanotubes (PD/CNT/GC) show excellent electrocatalytic activities towards the oxidation of NADH (catalytic reaction rate constant, k(h) = 7.26 × 10(3) M(-1) s(-1)), so the determination of NADH can be achieved with a high sensitivity of 8.77 μA mM(-1) under the potential of 0.0 V with minimal interference. We also develop an amperometric ethanol biosensor by integration of alcohol dehydrogenase (ADH) within the redox carbon nanotubes (PD/CNT/GC). The ethanol biosensor exhibits a wide linear range up to 7 mM with a lower detection limit of 0.30 mM as well as a high sensitivity of 10.85 nA mM(-1).
School of Chemistry, Northeast Normal University, Changchun, 130024, Jilin, China.
Xuyan
X
Mao
Yanhui
Y
Wu
Lanlan
L
Xu
Xiaojuan
X
Cao
Xiujun
X
Cui
Liande
L
Zhu
D000426:Alcohol Dehydrogenase / Q000378:metabolism; D015374:Biosensing Techniques / Q000379:methods*; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D009243:NAD / Q000032:analysis*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D010084:Oxidation-Reduction; D010618:Phenanthrolines / Q000737:chemistry*; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D012680:Sensitivity and Specificity
D037742:Nanotubes, Carbon; D010618:Phenanthrolines; D009243:NAD; C037153:1,10-phenanthroline-5,6-dione; D000426:Alcohol Dehydrogenase