Spectrofluorimetric method for determination and validation of cefixime in pharmaceutical preparations through derivatization with 2-cyanoacetamide.
A simple, sensitive and accurate method has been developed for spectrofluorimetric determination of cefixime in pure form and pharmaceutical preparations. The method is based on the reaction of cefixime with 2-cyanoacetamide in the presence of 21% ammonia at 100 °C. The fluorescent reaction product showed maximum fluorescence intensity at λ 378 nm after excitation at λ 330 nm. The factors affecting the derivatization reaction were carefully studied and optimized. The fluorescence intensity versus concentration plot was rectilinear over the range of 0.02 to 4 μg mL(-1) with correlation coefficient of 0.99036. The limit of detection (LOD) and limit of quantification (LOQ) was found to be 2.95 ng mL(-1) and 9.84 ng mL(-1), respectively. The proposed method was validated statistically and through recovery studies. The method was successfully applied for the determination of cefixime in pure and dosage form with percent recoveries from 98.117% to 100.38%. The results obtained from the proposed method have been compared with the official HPLC method and good agreement was found between them.
Shah
Jasmin
J
Institute of Chemical Sciences, University of Peshawar, Peshawar, Pakistan. jasminshah2001@yahoo.com
Jan
M Rasul
MR
Shah
Sultan
S
Inayatullah
D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000737:chemistry*; D020682:Cefixime / Q000032:analysis* / Q000737:chemistry*; D004339:Drug Compounding / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents / Q000737:chemistry; D009570:Nitriles / Q000737:chemistry*; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013696:Temperature; D013997:Time Factors
D000900:Anti-Bacterial Agents; D007202:Indicators and Reagents; D009570:Nitriles; D020682:Cefixime; C031594:2-cyanoacetamide