Biocatalytic separation of N-7/N-9 guanine nucleosides.
Vorbrüggen coupling of trimethylsilylated 2-N-isobutanoylguanine with peracetylated pentofuranose derivatives generally gives inseparable N-7/N-9 glycosyl mixtures. We have shown that the two isomers can be separated biocatalytically by Novozyme-435-mediated selective deacetylation of the 5'-O-acetyl group of peracetylated N-9 guanine nucleosides.
Singh
Sunil K
SK
Bioorganic Laboratory, Department of Chemistry, University of Delhi, Delhi-110 007, India.
Sharma
Vivek K
VK
Olsen
Carl E
CE
Wengel
Jesper
J
Parmar
Virinder S
VS
Prasad
Ashok K
AK
D055162:Biocatalysis; D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D006147:Guanine / Q000031:analogs & derivatives* / Q000737:chemistry*; D007536:Isomerism; D008049:Lipase / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D009705:Nucleosides / Q000737:chemistry*; D011684:Purine Nucleosides / Q000737:chemistry*
D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D009705:Nucleosides; D011684:Purine Nucleosides; D006147:Guanine; C093667:Novozyme 435; D008049:Lipase