Techniques for mammalian cell tissue culture.
This appendix opens with detailed discussions on the latest principles of sterile technique and preparation of culture media. Step-by-step protocols describe trypsinizing and subculturing monolayer cultures, passaging suspension cultures, freezing and thawing cells, counting cells using a hemacytometer, and preparing cells for transport.
Phelan
Mary C
MC
Molecular Pathology Laboratory Network, Maryville, Tennessee, USA.
D000818:Animals; D018929:Cell Culture Techniques / Q000639:trends; D002460:Cell Line; D002478:Cells, Cultured; D006801:Humans; D008322:Mammals; D014116:Toxicology / Q000379:methods