Micromass cultures in teratology.
This unit describes methods for culture of undifferentiated midbrain (mesencephalon) and limb bud cells from gestation day 12 rat embryos. When grown over 5 days in vitro, these mixed cell populations express many morphological, biochemical, molecular, and immunophenotypic characteristics observed during in vivo differentiation. These cultures can be used in a wide variety of studies designed to investigate normal cellular ontogeny, the teratogenic potential of test agents, or the mechanisms underlying the cellular response to environmental stress.
Ponce
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University of Washington, Seattle, Washington, USA.
D000818:Animals; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology / Q000187:drug effects; D046149:Embryo Culture Techniques; D004622:Embryo, Mammalian; D004785:Environmental Pollutants / Q000633:toxicity*; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects; D005260:Female; D018878:Limb Buds / Q000166:cytology / Q000187:drug effects*; D018811:Maternal Exposure / Q000009:adverse effects; D008636:Mesencephalon / Q000166:cytology / Q000187:drug effects*; D042282:Microdissection; D051381:Rats
D004785:Environmental Pollutants