Study on the influence of cross-sectional area and zeta potential on separation for hybrid-chip-based capillary electrophoresis using 3-D simulations.
Hybrid chips combing microchips with capillaries have displayed particular advantages in achieving UV-vis and mass spectroscopic detection. In this work, systematic 3-D numerical simulations have been carried out to explore the influence of junction interface cross-sectional area and ζ-potential distribution on sample band broadening in hybrid-chip electrophoresis separation. In this case, the ratio of cross-sectional area of chip to capillary channel (S(ratio) ) is used as the parameter of the variation in junction interface cross-sectional area. Theoretical simulations demonstrated that the decrease of the S(ratio) would increase the separation efficiency in the hybrid-chip-based CE with uniform ζ-potential distribution. ζ-potential distribution along the axial direction of the channel also affects mass transport in hybrid-chip-based CE. Therefore, the effect of ζ-potential distribution has been considered in the 3-D simulation. Theoretical simulation results reveal that ζ-potential distribution rather than the interface cross-sectional area variation (S(ratio) ) controls the sample band broadening and manipulates sample separation efficiency in the hybrid-chip-based CE with non-uniform ζ-potential distribution. Both the theoretical simulations and experimental results show that optimal hybrid-chip CE separation efficiency can be achieved at S(ratio) =1.
Wu
Zeng-Qiang
ZQ
Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, PR China.
Cao
Xiao-Dan
XD
Chen
Lu
L
Zhang
Jian-Rong
JR
Xia
Xing-Hua
XH
Fang
Qun
Q
Chen
Hong-Yuan
HY
D003198:Computer Simulation; D004574:Electromagnetic Fields; D046209:Electrophoresis, Microchip / Q000379:methods*; D020342:Finite Element Analysis; D008956:Models, Chemical