The impacts of bisphenol A (BPA) on abalone (Haliotis diversicolor supertexta) embryonic development.
The effects of bisphenol A (BPA) on abalone (Haliotis diversicolor supertexta) embryonic development were investigated by exposing the fertilized eggs to four different concentrations of BPA (0.05, 0.2, 2 and 10 μg mL(-1)). Toxicity endpoints including the embryo development parameters, the physiological features and the expression profile of several reference genes (prohormone convertase 1, PC1; cyclin B, CB; and cyclin-dependent kinase 1, CDK1) were assessed. The results showed that BPA could markedly reduce embryo hatchability, increase developmental malformation, and suppress the metamorphosis behavior of larvae. The possible toxicological mechanisms hidden behind of these effects (i.e. disturbing the embryogenesis) might result from three aspects: (1) BPA disturbance the cellular ionic homeostasis and osmoregulation of abalone embryos by changing the Na+-K+-ATPase and Ca2+-Mg2+-ATPase levels; (2) BPA induced oxidative damage of embryos by significantly altering the peroxidase (POD) activities and the malondialdehyde (MDA) production; and (3) the RT-PCR analysis further demonstrated that BPA perturbed the cellular endocrine regulation and cell cycle progression by down-regulating the PC1 gene, as well as over-expressing the CB and CDK1 genes. This is the first comprehensive study on the developmental toxicity of BPA to the marine abalone at morphological, physiological and molecular levels. The results in this study also indicated that the embryo tests can contribute to the ecological risk assessment of the endocrine disruptors in marine environment.
Zhou
Jin
J
Life Sciences Division, Graduate School at Shenzhen, Tsinghua University, Shenzhen, PR China.
Zhu
Xiao-Shan
XS
Cai
Zhong-Hua
ZH
D000818:Animals; D001559:Benzhydryl Compounds; D004625:Embryo, Nonmammalian / Q000187:drug effects*; D047108:Embryonic Development / Q000187:drug effects*; D052244:Endocrine Disruptors / Q000633:toxicity*; D049851:Gastropoda / Q000187:drug effects* / Q000196:embryology; D010636:Phenols / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000633:toxicity; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D052244:Endocrine Disruptors; D010636:Phenols; D014874:Water Pollutants, Chemical; C006780:bisphenol A; D000098826:Bisphenol A Compounds