Molecular genetics of Schizosaccharomyces pombe.
In this chapter we present basic protocols for the use of Schizosaccharomyces pombe, commonly known as fission yeast, in molecular biology and genetics research. Fission yeast is an increasingly popular model organism for the study of biological pathways because of its genetic tractability and as a model for metazoan biology. It provides an alternative and complimentary approach to Saccharomyces cerevisiae for addressing questions of cell biology, physiology, genetics, and genomics/proteomics. We include details and considerations for growing fission yeast, information on crosses and genetics, gene targeting and transformation, cell synchrony and analysis, and molecular biology protocols.
Sabatinos
Sarah A
SA
Department of Molecular and Computational Biology, University of Southern California, Los Angeles, California, USA.
Forsburg
Susan L
SL
D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D008967:Molecular Biology / Q000379:methods*; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D012568:Schizosaccharomyces / Q000166:cytology / Q000235:genetics*