Protein quantitation and analysis of purity.
The accurate quantitation of proteins and an analysis of their purity are essential in numerous areas of scientific research, and are a critical factor in many clinical applications. The large and varied number of techniques employed for this purpose is therefore not surprising. The selection of a suitable assay is dependent on factors such as the level of sensitivity required, the presence of interfering agents, and the composition of the protein itself. Here, protocols for the most commonly used protein determination methodologies are outlined, as well as for the more recently adapted technique of quantitative immuno-polymerase chain reaction.
School of Biotechnology and National Centre for Sensor Research, Dublin City University, Dublin, Ireland.
Eva M
EM
Campion
Sinéad T
ST
Loughran
Dermot
D
Walls
D000042:Absorption; D000818:Animals; D015153:Blotting, Western; D002138:Calibration; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D016133:Polymerase Chain Reaction; D011506:Proteins / Q000032:analysis* / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D011804:Quinolines / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D013194:Staining and Labeling
D011506:Proteins; D011804:Quinolines; C047117:bicinchoninic acid