[Cloning and characterization of Caveolin-1 gene in pigeon, Columba livia domestica].
Caveolins, a class of principal proteins forming the structure of caveolae in plasmalemma, were encoded by caveolins gene family. Caveolin-1 gene is a member of caveolins gene family. In the present study, a full-length of 2605 bp caveolin-1 cDNA sequence in Columba livia domestica, which included a 537 bp complete ORF encoding a 178 amino acids long putative peptide, were obtained by using RT-PCR and RACE technique. The Columba livia domestica caveolin-1 CDS shared 80.1% - 93.4% homology with Bos taurus, Canis lupus familiaris, Gallus gallus and Rattus norvegicus. Meanwhile, the putative amino acid sequence of Columba livia domestica caveolin-1 shared 85.4% - 97.2% homology with the above species. The semi-quantity RT-PCR revealed that Caveolin-1 expressions were detectable in all the Columba livia domestica tissues and the expressional level of caveolin-1 gene was high in adipose, medium in various muscles, low in liver. These results demonstrated that Caveolin-1 gene was potentially involved in some metabolic pathways in adipose and muscle.
College of Preclinical Medicine and Biological Science, Soochow University, Suzhou Jiangsu 215123, China.
Ying
Y
Zhang
Jian-Feng
JF
Yu
Li
L
Yang
Xing-Guo
XG
Wang
Zhi-Liang
ZL
Gu
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D051242:Caveolin 1 / Q000737:chemistry / Q000235:genetics*; D003001:Cloning, Molecular; D010856:Columbidae / Q000235:genetics*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction
D051242:Caveolin 1; D012333:RNA, Messenger