Luminescence targeting and imaging using a nanoscale generation 3 dendrimer in an in vivo colorectal metastatic rat model.
Surgery is currently the best approach for treating either primary or metastatic hepatic malignancies. Because only 20% of hepatic cancers are operable in patients, several types of regional therapy (RT) are emerging as alternate treatment modalities. However, RTs can have their own limitations at controlling tumor growth or may lack the ability to detect such metastases. Additional strategies can be implemented to enhance their efficacy. An animal model of hepatic metastases coupled with a gastroduodenal artery (GDA) cannulation technique may provide a site to apply such therapies. In our study, splenic injections were performed with CC531 adenocarcinoma cells, which generated metastatic hepatic tumors in WAG/RijHsd rats. Cannulation of GDA was achieved via a polyethylene catheter. Infusion of generation 3 polyamidoamine 4-amino-1,8-naphthalimide dendrimer containing 8 europium ions (Eu-G3P4A18N) via the GDA resulted in luminescence of the hepatic metastatic nodules. Imaging of the metastatic hepatic nodules was obtained with the help of a cooled charge coupled device (CCD) camera.


BACKGROUND
Hepatic malignancies represent a major therapeutic challenge, despite the available surgical and oncologic treatment modalities. In this paper, an animal model of hepatic adenocarcinoma is used in demonstrating successful targeting of spleen metastases with generation 3 polyamidoamine 4-amino-1,8-naphthalimide dendrimer containing 8 europium ions (Eu-G3P4A18N) for luminescence imaging.
Department of Surgery, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA.
Marco A
MA
Alcala
Shu Ying
SY
Kwan
Chad M
CM
Shade
Megan
M
Lang
Hyounsoo
H
Uh
Manyan
M
Wang
Stephen G
SG
Weber
David L
DL
Bartlett
Stéphane
S
Petoud
Yong J
YJ
Lee
D015057:1-Naphthylamine / Q000031:analogs & derivatives / Q000138:chemical synthesis / Q000737:chemistry / Q000493:pharmacokinetics; D000818:Animals; D002404:Catheterization; D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000175:diagnosis* / Q000556:secondary*; D050091:Dendrimers / Q000138:chemical synthesis / Q000737:chemistry / Q000493:pharmacokinetics; D003952:Diagnostic Imaging / Q000379:methods*; D004195:Disease Models, Animal; D005063:Europium; D008099:Liver / Q000473:pathology; D008113:Liver Neoplasms / Q000473:pathology; D049449:Luminescence; D053758:Nanoparticles; D053644:Naphthalimides / Q000138:chemical synthesis / Q000737:chemistry / Q000493:pharmacokinetics; D010316:Particle Size; D015363:Quinolones / Q000138:chemical synthesis / Q000737:chemistry / Q000493:pharmacokinetics; D051381:Rats; D013997:Time Factors
D050091:Dendrimers; D053644:Naphthalimides; D015363:Quinolones; C086538:4-amino-1,8-naphthalimide; D005063:Europium; D015057:1-Naphthylamine