[Effects of inhaled budesonide on the bronchial-pulmonary pathology and expression of thymic stromal lymphopoietin in lung tissues in asthmatic rats].
OBJECTIVE
Thymic stromal lymphopoietin (TSLP) plays an important role in initiating dendritic cell mediated allergic inflammation. This study was designed to examine the effects of inhaled budesonide on TSLP expression in the lung tissues and on the bronchial-pulmonary pathology in asthmatic rats.


METHODS
Thirty-two female Sprague-Dawley rats were sensitized and challenged with inhaled ovalbumin (OVA) to induce asthma. The asthmatic rats were randomly divided into 2 groups on the 22nd day of OVA challenge: a budesonide treatment group that received inhaled budesonide at 0.32 mg/kg daily for 7 days and an asthma control group that received inhaled 0.9% normal saline for 7 days. TSLP expression in the lung tissues was measured by Western blot and fluorescent-immunohistochemistry 29 and 36 days after OVA challenge. Bronchial-pulmonary pathological changes were evaluated by hematoxylin & eosin and periodic acid-schiff staining.


RESULTS
Budesonide treatment alleviated airway inflammation when compared with the asthma control group 29 days after OVA challenge. However, the airway inflammatory reactions were aggravated in the budesonide treatment group 36 days after OVA challenge (7 days after budesonide discontinuance). TSLP expression in the lung tissues was significantly lower in the budesonide treatment group than that in the asthma control group both 29 and 36 days after OVA challenge (P<0.05).


CONCLUSIONS
Inhaled budesonide can inhibit the TSLP expression in the lung tissues and alleviate lung inflammatory reactions in asthmatic rats, but there is end-of-dose failure.
Department of Pediatrics, Anhui Provincial Hospital, Hefei, China.
Hui
H
Liu
Jia-Hua
JH
Pan
Wan-Ling
WL
Lou
Jin-Gen
JG
He
D000280:Administration, Inhalation; D000818:Animals; D001249:Asthma / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D015153:Blotting, Western; D001980:Bronchi / Q000473:pathology*; D019819:Budesonide / Q000008:administration & dosage*; D016207:Cytokines / Q000032:analysis*; D005260:Female; D007150:Immunohistochemistry; D008168:Lung / Q000473:pathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D000094632:Thymic Stromal Lymphopoietin
D016207:Cytokines; D019819:Budesonide; D000094632:Thymic Stromal Lymphopoietin; C000721968:TSLP protein, rat