Kidney from uncontrolled donors after cardiac death with one hour warm ischemic time: resuscitation by extracorporal normothermic abdominal perfusion "in situ" by leukocytes-free oxygenated blood.
The availability of brain death donors is restricted by many factors. Use of uncontrolled donors after cardiac death could be a promising perspective, but the limiting factor in uncontrolled donation after cardiac death is the warm ischemic time. The purpose of our work was to develop an in situ kidney preservation protocol with application of the extracorporal normothermic abdominal perfusion for organ resuscitation in uncontrolled donors after cardiac death. The main attention was paid to the elimination of leukocytes as the key damaging factor from modified donor oxygenated blood circulating in the device. In 2009, we had 10 uncontrolled donors with warm ischemic time from 45 to 92 min; a normothermic extracorporal perfusion device was applied, providing preservation and restoration of kidney after ischemic damage. In 6 out of 20 kidney recipients, graft function was recovered immediately. All kidney grafts are functioning, and to the end of the third month, the average creatinine was 118.5 ± 19.9 mM. Treatment of ischemically damaged kidney by normothermic extracorporal perfusion with leukocyte depletion before procurement seems to be a challenging protocol for expanding donors' pool and demands further study.
Transplant Department, St. Petersburg State Research Institute for Emergency, St. Petersburg, Russia. onreznik@yahoo.com
Oleg
O
Reznik
Andrej
A
Skvortsov
Igor
I
Loginov
Alexey
A
Ananyev
Sergey
S
Bagnenko
Yan
Y
Moysyuk
D000328:Adult; D000368:Aged; D001926:Brain Death; D003404:Creatinine; D003643:Death; D005112:Extracorporeal Circulation; D006085:Graft Survival; D006801:Humans; D007668:Kidney / Q000503:physiopathology*; D016030:Kidney Transplantation; D047589:Leukocyte Reduction Procedures; D008875:Middle Aged; D009926:Organ Preservation / Q000379:methods*; D010100:Oxygen / Q000378:metabolism; D010477:Perfusion; D014019:Tissue Donors; D009927:Tissue and Organ Procurement; D052096:Warm Ischemia
D003404:Creatinine; D010100:Oxygen