Pharmacokinetics of 5-aminofluorescein-albumin, a novel fluorescence marker of brain tumors during surgery.
In a phase I/II study, the new fluorescence marker 5-aminofluorescein covalently bound to human serum albumin (AFL-HSA) was intravenously administered to 10 patients with brain tumor 1 to 4 days before surgery, and AFL-HSA kinetics were determined to assess the optimum timing of dye administration. AFL-HSA was determined in serum and brain tissue by size exclusion chromatography with fluorescence detection. AFL-HSA disposition was quite similar to albumin kinetics with small volumes of distribution (volume of central compartment [V(c)] = 2.4 L; volume of distribution [Vd(β)] = 6.8 L; volume at steady state [V(ss)] = 5.9 L), a low clearance (mean Cl = 16.85 mL/h), and a long elimination half-life (mean T(1/2;β) = 307 hours). Predicted peak concentrations in the peripheral compartment occurred at 80.9 hours, the time point of optimal fluorescence intensity observed in brain tumors during surgery.
Ding
Reinhard
R
Department of Clinical Pharmacology and Pharmaco-epidemiology, University of Heidelberg, Heidelberg, Germany.
Frei
Eva
E
Fardanesh
Mahmoudreza
M
Schrenk
Hans-Hermann
HH
Kremer
Paul
P
Haefeli
Walter E
WE
D000368:Aged; D001932:Brain Neoplasms / Q000378:metabolism / Q000601:surgery*; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D005260:Female; D005452:Fluoresceins / Q000008:administration & dosage / Q000493:pharmacokinetics*; D005456:Fluorescent Dyes / Q000008:administration & dosage / Q000493:pharmacokinetics*; D005858:Germany; D006207:Half-Life; D006801:Humans; D007262:Infusions, Intravenous; D008297:Male; D008657:Metabolic Clearance Rate; D008875:Middle Aged; D008954:Models, Biological; D015336:Molecular Probe Techniques; D016343:Monitoring, Intraoperative / Q000379:methods*; D019635:Neurosurgical Procedures; D012709:Serum Albumin / Q000008:administration & dosage / Q000493:pharmacokinetics*; D013050:Spectrometry, Fluorescence; D014018:Tissue Distribution
D005452:Fluoresceins; D005456:Fluorescent Dyes; D012709:Serum Albumin; C030864:5-aminofluorescein