High-throughput SHAPE and hydroxyl radical analysis of RNA structure and ribonucleoprotein assembly.
RNA folds to form complex structures vital to many cellular functions. Proteins facilitate RNA folding at both the secondary and tertiary structure levels. An absolute prerequisite for understanding RNA folding and ribonucleoprotein (RNP) assembly reactions is a complete understanding of the RNA structure at each stage of the folding or assembly process. Here we provide a guide for comprehensive and high-throughput analysis of RNA secondary and tertiary structure using SHAPE and hydroxyl radical footprinting. As an example of the strong and sometimes surprising conclusions that can emerge from high-throughput analysis of RNA folding and RNP assembly, we summarize the structure of the bI3 group I intron RNA in four distinct states. Dramatic structural rearrangements occur in both secondary and tertiary structure as the RNA folds from the free state to the active, six-component, RNP complex. As high-throughput and high-resolution approaches are applied broadly to large protein-RNA complexes, other proteins previously viewed as making simple contributions to RNA folding are also likely to be found to exert multifaceted, long-range, cooperative, and nonadditive effects on RNA folding. These protein-induced contributions add another level of control, and potential regulatory function, in RNP complexes.
Department of Chemistry, University of North Carolina, Chapel Hill, North Carolina, USA.
Jennifer L
JL
McGinnis
Caia D S
CD
Duncan
Kevin M
KM
Weeks
D018076:DNA, Complementary / Q000737:chemistry; D019075:Electrophoresis, Capillary; D017665:Hydroxyl Radical / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D012313:RNA / Q000737:chemistry*; D012261:Ribonucleoproteins / Q000737:chemistry*
D018076:DNA, Complementary; D012261:Ribonucleoproteins; D017665:Hydroxyl Radical; D012313:RNA