The regeneration of transected sciatic nerves of adult rats using chitosan nerve conduits seeded with bone marrow stromal cell-derived Schwann cells.
Autologous nerve grafts have been the 'gold standard' for treatment of peripheral nerve defects that exceed the critical gap length. To address issues of limited availability of donor nerves and donor site morbidity, we have fabricated chitosan conduits and seeded them with bone marrow stromal cell (BMSC)-derived Schwann cells as an alternative. The derived Schwann cells used were checked for fate commitment. The conduits were tested for efficacy in bridging the critical gap length of 12 mm in sciatic nerves of adult rats. By three months post-operation, mid-shank circumference, nerve conduction velocity, average regenerated myelin area, and myelinated axon count, in nerves bridged with BMSC-derived Schwann cells were similar to those treated with sciatic nerve-derived Schwann cells (p > 0.05) but significantly higher than those bridged with PBS-filled conduits (p < 0.05). Evidence is thus provided in support of the use of chitosan conduits seeded with BMSC-derived Schwann cells to treat critical defects in peripheral nerves. This provides the basis to pursue BMSC as an autologous source of Schwann cells for transplantation therapy in larger animal species.
Department of Physiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, PR China.
Qiang
Q
Ao
Chun-Kit
CK
Fung
Alex Yat-Ping
AY
Tsui
Sa
S
Cai
Huan-Cong
HC
Zuo
Ying-Shing
YS
Chan
Daisy Kwok-Yan
DK
Shum
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry*; D048091:Guided Tissue Regeneration / Q000379:methods*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D009416:Nerve Regeneration / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012583:Schwann Cells; D012584:Sciatic Nerve / Q000166:cytology*; D023822:Tissue Engineering / Q000379:methods*
D048271:Chitosan