DNA hybridization sensor based on pentacene thin film transistor.
A DNA hybridization sensor using pentacene thin film transistors (TFTs) is an excellent candidate for disposable sensor applications due to their low-cost fabrication process and fast detection. We fabricated pentacene TFTs on glass substrate for the sensing of DNA hybridization. The ss-DNA (polyA/polyT) or ds-DNA (polyA/polyT hybrid) were immobilized directly on the surface of the pentacene, producing a dramatic change in the electrical properties of the devices. The electrical characteristics of devices were studied as a function of DNA immobilization, single-stranded vs. double-stranded DNA, DNA length and concentration. The TFT device was further tested for detection of λ-phage genomic DNA using probe hybridization. Based on these results, we propose that a "label-free" detection technique for DNA hybridization is possible through direct measurement of electrical properties of DNA-immobilized pentacene TFTs.
Kim
Jung-Min
JM
Department of Nano Science & Engineering, Myongji University, Gyeonggi-do 449-728, Republic of Korea.
Jha
Sandeep Kumar
SK
Chand
Rohit
R
Lee
Dong-Hoon
DH
Kim
Yong-Sang
YS
D015374:Biosensing Techniques / Q000295:instrumentation*; D003217:Conductometry / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D017403:In Situ Hybridization / Q000295:instrumentation*; D008567:Membranes, Artificial; D009279:Naphthacenes / Q000737:chemistry*; D014173:Transistors, Electronic
D008567:Membranes, Artificial; D009279:Naphthacenes; C523499:pentacene