Rubisco activase activity assays.
Ribulose-1,5-bisphosphate (RuBP) carboxylase/oxygenase (Rubisco) activase functions as a mechano-chemical motor protein using the energy from ATP hydrolysis to contort the structure of its target protein, Rubisco. This action modulates the activation state of Rubisco by removing tightly-bound inhibitory sugar-phosphates from Rubisco's catalytic sites, thereby restoring the sites to catalytic competence. This chapter reports methods developed for assaying the two activities of Rubisco activase: ATP hydrolysis and Rubisco activation.
Barta
Csengele
C
U.S. Department of Agriculture, Arid-Land Agricultural Research Center, Maricopa, AZ, USA.
Carmo-Silva
A Elizabete
AE
Salvucci
Michael E
ME
D000255:Adenosine Triphosphate / Q000378:metabolism; D004789:Enzyme Activation; D057075:Enzyme Assays / Q000379:methods*; D006868:Hydrolysis; D018515:Plant Leaves / Q000201:enzymology; D010940:Plant Proteins / Q000378:metabolism*; D012273:Ribulose-Bisphosphate Carboxylase / Q000378:metabolism; D012274:Ribulosephosphates / Q000378:metabolism
D010940:Plant Proteins; D012274:Ribulosephosphates; C054765:rca protein, plant; C001933:ribulose-1,5 diphosphate; D000255:Adenosine Triphosphate; D012273:Ribulose-Bisphosphate Carboxylase