Lack of association between the Thr431Asn and Arg83Lys polymorphisms of the ROCK2 gene and diabetic retinopathy.
OBJECTIVE
To analyze the genotype distributions and allele frequencies for ROCK2 Thr431Asn and Arg83Lys polymorphisms among the diabetic retinopathy patients in a Turkish population.


METHODS
In this case-control study, 335 patients with diabetes mellitus were recruited and divided into three groups according to non-proliferative (n = 127), proliferative (n = 85) diabetic retinopathy, and no retinopathy (n = 123, served as a diabetic control group). Genomic DNA from the patients, and the nondiabetic healthy control cases (n = 132) was analyzed by real-time PCR using a Light-Cycler.


RESULTS
Neither genotype distributions nor the allele frequencies for the Thr431Asn or Arg83Lys polymorphisms showed a significant difference between the groups. The haplotypes were also not significantly associated with diabetic retinopathy.


CONCLUSIONS
These results suggest that there were no evidence for an association of ROCK2 gene Thr431Asn and Arg83Lys polymorphisms with diabetes or diabetic retinopathy in the Turkish population.
Department of Pharmacology, Faculty of Medicine, University of Gaziantep, Gaziantep, Turkey. demiryurek@gantep.edu.tr
Abdullah T
AT
Demiryurek
Ibrahim
I
Erbagci
Serdar
S
Oztuzcu
Belgin
B
Alasehirli
Esma
E
Ozkara
Mehmet
M
Seker
Ayhan
A
Sönmez
Muge
M
Ozsan
Celalettin
C
Camci
D000328:Adult; D000368:Aged; D000596:Amino Acids / Q000235:genetics*; D003930:Diabetic Retinopathy / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D055106:Genome-Wide Association Study; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic / Q000235:genetics*; D014421:Turkey; D054460:rho-Associated Kinases / Q000235:genetics*
D000596:Amino Acids; C515852:ROCK2 protein, human; D054460:rho-Associated Kinases