Measurement of B-type natriuretic peptide by two assays utilizing antibodies with different epitope specificity.
OBJECTIVE
To compare plasma BNP values determined by a conventional-type and a novel-type of BNP assays.


METHODS
Plasma samples (n=94) from HF patients were analyzed by the novel-type "Single Epitope Sandwich" (SES assay prototype) and the conventional-type Siemens ADVIA Centaur BNP assays. Both assays were calibrated using recombinant proBNP (expressed in E. coli).


RESULTS
The SES assay measured 1.2- to 7.2-fold (2.1±0.9; mean, SD) greater BNP concentrations compared to the Siemens assay. A subset of six samples (6.4%) demonstrated the largest (3- to 7.2-fold higher) difference.


CONCLUSIONS
The SES prototype assay appears to more accurately measure the absolute concentrations of BNP immunoreactive forms.
HyTest LTD, Intelligate, 6th floor, Joukahaisenkatu 6, 20520 Turku, Finland.
Natalia N
NN
Tamm
Alexander G
AG
Semenov
Karina R
KR
Seferian
Anastasia V
AV
Bereznikova
Maryann M
MM
Murakami
Fred S
FS
Apple
Ekaterina V
EV
Koshkina
Mihail I
MI
Krasnoselsky
Alexey G
AG
Katrukha
D000906:Antibodies; D000939:Epitopes / Q000276:immunology; D004926:Escherichia coli; D006801:Humans; D020097:Natriuretic Peptide, Brain / Q000097:blood; D010446:Peptide Fragments / Q000097:blood; D012680:Sensitivity and Specificity
D000906:Antibodies; D000939:Epitopes; D010446:Peptide Fragments; D020097:Natriuretic Peptide, Brain