Production and characterization of engineered alginate-based microparticles containing ECM powder for cell/tissue engineering applications.
A method for the production of engineered alginate-based microparticles, containing extracellular matrix and neonatal porcine Sertoli cells (SCs), is described. As a source for extracellular matrix, a powder form of isolated and purified urinary bladder matrix (UBM) was employed. We demonstrated that the incorporation of UBM does not significantly alter the morphological and dimensional characteristics of the microparticles. The alginate microparticles were used for SC encapsulation as an immunoprotective barrier for transplant purposes, while the co-entrapped UBM promoted retention of cell viability and function. These engineered microparticles could represent a novel approach to enhancing immunological acceptance and increasing the functional life-span of the entrapped cells for cell/tissue engineering applications. In this respect, it is noteworthy that isolated neonatal porcine SCs, administered alone in highly biocompatible microparticles, led to diabetes prevention and reversion in nonobese diabetic (NOD) mice.
Department of Chemistry and Technology of Drugs, University of Perugia, Perugia, Italy.
Stefania
S
Mazzitelli
Giovanni
G
Luca
Francesca
F
Mancuso
Mario
M
Calvitti
Riccardo
R
Calafiore
Claudio
C
Nastruzzi
Scott
S
Johnson
Stephen F
SF
Badylak
D000464:Alginates / Q000737:chemistry*; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D005109:Extracellular Matrix; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D008297:Male; D008863:Microspheres; D011208:Powders; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine; D023822:Tissue Engineering; D001743:Urinary Bladder
D000464:Alginates; D017931:DNA Primers; D006603:Hexuronic Acids; D011208:Powders; D020723:Glucuronic Acid