Influence of serum and necrotic soft tissue on the antimicrobial effects of intracanal medicaments.
The purpose of this study was to investigate the influence of serum and necrotic soft tissue on the antimicrobial activity of intracanal medicaments. The medicaments tested were: calcium hydroxyde/glycerin paste, calcium hydroxide/chlorhexidine paste, calcium hydroxide/camphorated paramonochlorophenol/glycerin paste, and chlorhexidine/zinc oxide paste. Survival of Enterococcus faecalis and Candida albicans exposed to the medicaments tested in the presence or absence of serum or necrotic tissue was monitored in three in vitro experiments where samples for culturing were taken at different time periods. The overall results demonstrated that the antimicrobial activity of all intracanal medicaments tested was slowed down in the presence of necrotic tissue. Calcium hydroxide pastes in glycerin or chlorhexidine were significantly affected by serum. Of the medicaments tested in this study, the least affected was the calcium hydroxide/camphorated paramonochlorophenol/glycerin paste.
Department of Endodontics, Dental School, Estácio de Sá University, Rio de Janeiro, RJ, Brazil.
Julio C Machado de
JC
Oliveira
Flávio R F
FR
Alves
Milton de
Md
Uzeda
Isabela N
IN
Rôças
José F
JF
Siqueira
D000818:Animals; D000891:Anti-Infective Agents, Local / Q000737:chemistry / Q000494:pharmacology*; D002126:Calcium Hydroxide / Q000737:chemistry / Q000494:pharmacology; D002164:Camphor / Q000737:chemistry / Q000494:pharmacology; D002176:Candida albicans / Q000187:drug effects*; D002417:Cattle; D002710:Chlorhexidine / Q000737:chemistry / Q000494:pharmacology; D002733:Chlorophenols / Q000737:chemistry / Q000494:pharmacology; D015169:Colony Count, Microbial; D003786:Dental Pulp Cavity / Q000382:microbiology*; D004338:Drug Combinations; D013293:Enterococcus faecalis / Q000187:drug effects*; D005990:Glycerol / Q000737:chemistry / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D009336:Necrosis / Q000382:microbiology; D012388:Root Canal Irrigants / Q000737:chemistry / Q000494:pharmacology*; D044967:Serum; D016369:Smear Layer; D015034:Zinc Oxide / Q000737:chemistry / Q000494:pharmacology
D000891:Anti-Infective Agents, Local; D002733:Chlorophenols; D004338:Drug Combinations; D012388:Root Canal Irrigants; D002164:Camphor; C029804:camphorated parachlorophenol; D005990:Glycerol; D002126:Calcium Hydroxide; D002710:Chlorhexidine; D015034:Zinc Oxide