Chemo-microbial conversion of cellulose into polyhydroxybutyrate through ruthenium-catalyzed hydrolysis of cellulose into glucose.
Cellulose-derived glucose generated using the supported ruthenium catalyst was applied to poly(3-hydroxybutyrate) [P(3HB)] production in recombinant Escherichia coli. By the reaction with the catalyst at 220°C, 15-20 carbon mol% of cellulose was converted into glucose. The hydrolysate also contained byproducts such as fructose, mannose, levoglucosan, oligomeric cellulose, 5-hydroxymethylfurfural (5-HMF), and furfural together with unidentified compounds. Setting the reaction temperature lower (215°C) improved the ratio of glucose to 5-HMF, which was a main inhibiting factor for the cell growth. Indeed, the recombinant E. coli exhibited better performance on the hydrolysate generated at 215°C and accumulated P(3HB) up to 42 wt%, which was the same as the case of the same concentration of analytical grade glucose. The result indicated that the ruthenium-mediated cellulose hydrolysis has a potency as a useful biorefinery process for production of bio-based plastic from cellulosic biomass.
Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, Kita-ku, Sapporo, Japan.
Ken'ichiro
K
Matsumoto
Hirokazu
H
Kobayashi
Koji
K
Ikeda
Tasuku
T
Komanoya
Atsushi
A
Fukuoka
Seiichi
S
Taguchi
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002482:Cellulose / Q000737:chemistry* / Q000378:metabolism*; D004926:Escherichia coli / Q000502:physiology*; D005947:Glucose / Q000737:chemistry* / Q000378:metabolism*; D006868:Hydrolysis; D006885:Hydroxybutyrates / Q000138:chemical synthesis / Q000378:metabolism*; D011091:Polyesters / Q000138:chemical synthesis / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D012428:Ruthenium / Q000737:chemistry*; D000094122:Polyhydroxybutyrates
D001426:Bacterial Proteins; D002482:Cellulose; D005947:Glucose; D006885:Hydroxybutyrates; D011091:Polyesters; D011994:Recombinant Proteins; D012428:Ruthenium; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates