Selective labeling of polypeptides using protein farnesyltransferase via rapid oxime ligation.
An aldehyde-containing alternative substrate for protein farnesyltransferase was prepared and shown to be enzymatically incorporated into a peptide and a protein. The protein was subsequently immobilized onto aminooxy-functionalized agarose beads or labeled with a fluorophore. This method for protein modification provides an alternative to the commonly employed Cu(I)-catalyzed click reaction.
Department of Chemistry, University of Minnesota, 207 Pleasant Street, S. E. Minneapolis, MN 55455, USA.
Mohammad
M
Rashidian
Jonathan K
JK
Dozier
Stepan
S
Lenevich
Mark D
MD
Distefano
D000447:Aldehydes / Q000737:chemistry; D019883:Alkyl and Aryl Transferases / Q000378:metabolism*; D055162:Biocatalysis; D003300:Copper / Q000737:chemistry*; D010091:Oximes / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization
D000447:Aldehydes; D010091:Oximes; D010455:Peptides; D003300:Copper; D019883:Alkyl and Aryl Transferases; C064911:p21(ras) farnesyl-protein transferase