Generation of protein and cell microarrays on functionalized surfaces.
The technique of selective immobilization of biomolecules in defined positions or areas using a simple procedure is essential for various applications such as biosensors, biochips, biomedical microdevices, and tissue engineering. For the generation of biomolecule microarrays, it is necessary to develop a functional surface retaining protein functionality and cell viability, and an efficient patterning tool having flexibility of size and shape. In this chapter, we have presented the simple tools of protein and cell microarray based on functionalized surface such as a spotting method with improvement of protein functionality, a functionalized silicon-based surface using photolithography, and an orthogonally polyelectrolyte-coated surface based on soft-lithography.
Choi
Yoo Seong
YS
Department of Chemical Engineering, Pohang University of Science and Technology, Pohang, Korea.
Lee
Chang-Soo
CS
D000818:Animals; D004867:Equipment Design; D006801:Humans; D055616:Microtechnology / Q000379:methods*; D011108:Polymers / Q000737:chemistry*; D040081:Protein Array Analysis / Q000295:instrumentation*; D011506:Proteins / Q000737:chemistry*; D012825:Silicon / Q000737:chemistry*; D013499:Surface Properties; D046888:Tissue Array Analysis / Q000295:instrumentation*
D011108:Polymers; D011506:Proteins; D012825:Silicon