Gel-filtration chromatography.
Gel-filtration chromatography is a popular and versatile technique that permits the effective separation of proteins and other biological molecules in high yield. Here, the basis of the method is described and typical matrix types are contrasted. The selection of suitable operating conditions and applications of the method are also discussed.
O'Fágáin
Ciarán
C
School of Biotechnology and National Centre for Sensor Research, Dublin City University, Dublin, Ireland. ciaran.fagan@dcu.ie
Cummins
Philip M
PM
O'Connor
Brendan F
BF
D002469:Cell Separation; D002850:Chromatography, Gel / Q000379:methods*; D006801:Humans; D046911:Macromolecular Substances / Q000737:chemistry / Q000302:isolation & purification; D008970:Molecular Weight; D011092:Polyethylene Glycols / Q000737:chemistry; D014780:Viruses / Q000302:isolation & purification
D046911:Macromolecular Substances; D011092:Polyethylene Glycols