Controlled stoichiometric synthesis of DNA-quantum dot conjugates using Ni-mediated coordination chemistry.
An oligonucleotide modified with Ni-nitrilotriacetate (NTA) was successfully synthesized and used for the stoichiometric functionalization of QDs. This synthetic approach allowed for the facile preparation of DNA-QD conjugates with a defined DNA/QD ratio using well-known Ni-histidine coordination chemistry. A FRET based DNA-QD nanoprobe was prepared using this method highlighting the great potential of this synthetic strategy.
Nano/Bio Chemistry Laboratory, Institut Pasteur Korea (IP-K), 696 Sampyeong-dong, Bundang-gu, Seongnam-si, Gyeonggi-do, Korea.
Haejin
H
Kwon
Sukmin
S
Hong
Heeyeon
H
Kim
Youngseon
Y
Choi
Jaeseung
J
Kim
Rita
R
Song
D031541:Fluorescence Resonance Energy Transfer; D006639:Histidine / Q000737:chemistry; D009532:Nickel / Q000737:chemistry*; D009571:Nitrilotriacetic Acid / Q000737:chemistry; D009841:Oligonucleotides / Q000737:chemistry*; D045663:Quantum Dots
D009841:Oligonucleotides; D006639:Histidine; D009532:Nickel; D009571:Nitrilotriacetic Acid