Effect of antibiotic treatment on bacteriophage production by a cystic fibrosis epidemic strain of Pseudomonas aeruginosa.
Phage production in response to antibiotics varied among four isolates of a Pseudomonas aeruginosa cystic fibrosis (CF) epidemic strain. Whereas ciprofloxacin induced higher levels of phage production, other CF-relevant antibiotics led to reduced production. We detected free phages directly in CF patient sputum samples by both plaque (40% positive) and PCR (76% positive) assays. Our observations suggest that the choice of antibiotics could influence the number of free phages within the CF lung environment.
NIHR Biomedical Research Centre in Microbial Disease, Royal Liverpool University Hospital, Liverpool, United Kingdom.
Joanne L
JL
Fothergill
Eilidh
E
Mowat
Martin J
MJ
Walshaw
Martin J
MJ
Ledson
Chloe E
CE
James
Craig
C
Winstanley
D001435:Bacteriophages / Q000187:drug effects* / Q000235:genetics; D003550:Cystic Fibrosis / Q000382:microbiology*; D006801:Humans; D016133:Polymerase Chain Reaction; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000472:pathogenicity / Q000821:virology*