Cellular responses in skeletal muscle to a season of ice hockey.
We hypothesized that a season of ice hockey would result in extensive remodeling of muscle. Tissue sampled from the vastus lateralis of 15 players (age = 20.6 ± 0.4 years; mean ± SE) prior to (PRE) and following (POST) a season was used to characterize specific adaptations. Measurement of representative metabolic pathway enzymes indicated higher maximal activities in POST than in PRE (p < 0.05) for succinic dehydrogenase (3.26 ± 0.31 vs. 3.91 ± 0.11 mol mg protein(-1) min(-1)), citrate synthase (7.26 ± 0.70 vs. 8.70 ± 0.55 mol mg protein(-1) min(-1)), and phosphofructokinase (12.8 ± 1.3 vs. 14.4 ± 0.96 mol mg protein(-1) min(-1)) only. The season resulted in an increase in Na+-K+-ATPase concentration (253 ± 6.3 vs. 265 ± 6.0 pmol g(-1) wet weight), a decrease (p < 0.05) in maximal activity of the sarcoplasmic reticulum Ca2+-ATPase (107 ± 4.2 micromol g protein(-1) min(-1) vs. 92.0 ± 4.6 micromol g protein(-1) min(-1)), and no change in the distribution (%) of fibre types. A smaller (p < 0.05) cross-sectional area (CSA) for both type I (-11.7%) and type IIA (-18.2%) fibres and a higher (p < 0.05) capillary count/CSA for type I (+17.9%) and type IIA (+17.2%) were also found over the season. No changes were found in peak oxygen consumption (51.4 ± 1.2 mL kg(-1) min(-1) vs. 52.3 ± 1.3 mL kg(-1) min(-1)). The results suggest, based on the alterations in oxidative and perfusion potentials and muscle mass, that the dominant adaptations are in support of oxidative metabolism, which occurs at the expense of fibre CSA and possibly force-generating potential.
Department of Kinesiology, University of Waterloo, Waterloo, ON N2L 3G1, Canada. green@healthy.uwaterloo.ca
Howard J
HJ
Green
Aziz
A
Batada
Bill
B
Cole
Margaret E
ME
Burnett
Helen
H
Kollias
Scott
S
McKay
Brian
B
Roy
John
J
Schertzer
Ian
I
Smith
Susan
S
Tupling
D000222:Adaptation, Physiological / Q000502:physiology*; D000328:Adult; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D002950:Citrate (si)-Synthase / Q000378:metabolism; D004734:Energy Metabolism / Q000502:physiology*; D015444:Exercise / Q000502:physiology; D006688:Hockey / Q000502:physiology*; D006801:Humans; D008297:Male; D018485:Muscle Fibers, Skeletal / Q000201:enzymology; D009124:Muscle Proteins / Q000378:metabolism; D018482:Muscle, Skeletal / Q000166:cytology* / Q000502:physiology*; D010101:Oxygen Consumption / Q000502:physiology; D025203:Phosphofructokinase-1, Muscle Type / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013385:Succinate Dehydrogenase / Q000378:metabolism
D009124:Muscle Proteins; D013385:Succinate Dehydrogenase; D002950:Citrate (si)-Synthase; D025203:Phosphofructokinase-1, Muscle Type; D000252:Calcium-Transporting ATPases; D000254:Sodium-Potassium-Exchanging ATPase