Localized IR spectroscopy of hemoglobin.
IR absorption spectroscopy of hemoglobin was performed using an infrared (IR) optical parametric oscillator laser and a commercial atomic force microscope (AFM) in a novel experimental arrangement based on the use of a bottom-up excitation alignment. This experimental approach enables detection of protein samples with resolution much higher than that of standard IR spectroscopy. Presented here are AFM-based IR absorption spectra of micron-sized hemoglobin features.
NanoPhotonics Research Group, School of Physics, University College Dublin, Belfield, Dublin, Ireland.
Fiona
F
Yarrow
James H
JH
Rice
D006454:Hemoglobins / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D007834:Lasers; D018625:Microscopy, Atomic Force / Q000379:methods; D009991:Oscillometry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D017550:Spectroscopy, Fourier Transform Infrared / Q000295:instrumentation / Q000379:methods*; D013997:Time Factors
D006454:Hemoglobins