Cell-death-mode switch from necrosis to apoptosis in hydrogen peroxide treated macrophages.
Cell death is typically defined either as apoptosis or necrosis. Because the consequences of apoptosis and necrosis are quite different for an entire organism, the investigation of the cell-death-mode switch has considerable clinical significance. The existence of a necrosis-to-apoptosis switch induced by hydrogen peroxide in macrophage cell line RAW 264.7 cells was confirmed by using flow cytometry and fluorescence microscopy. With the help of computational simulations, this study predicted that negative feedbacks between NF-κB and MAPKs are implicated in converting necrosis into apoptosis in macrophages exposed to hydrogen peroxide, which has significant implications.
Lin
XuZhu
X
Department of Biochemistry, Nanjing University, Nanjing, 210093, China.
Sun
TingZhe
T
Cai
MeiHong
M
Shen
PingPing
P
D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D016923:Cell Death / Q000187:drug effects* / Q000502:physiology; D002460:Cell Line; D003198:Computer Simulation; D025461:Feedback, Physiological; D005434:Flow Cytometry; D006861:Hydrogen Peroxide / Q000633:toxicity*; D020935:MAP Kinase Signaling System / Q000187:drug effects; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D008856:Microscopy, Fluorescence; D008954:Models, Biological; D016328:NF-kappa B / Q000378:metabolism; D009336:Necrosis
D016328:NF-kappa B; D006861:Hydrogen Peroxide