Intracellular ethanol accumulation in yeast cells during aerobic fermentation: a Raman spectroscopic exploration.
OBJECTIVE
To investigate the intracellular ethanol accumulation in yeast cells by using laser tweezers Raman spectroscopy (LTRS).


RESULTS
Ethanol accumulation in individual yeast cells during aerobic fermentation triggered by excess glucose was studied using LTRS. Its amount was obtained by comparing intracellular and extracellular ethanol concentrations during initial process of ethanol production. We found that (i) yeasts start to produce ethanol within 3 min after triggering aerobic fermentation, (ii) average ratio of intracellular to extracellular ethanol is 1.54 ± 0.17 during the initial 3 h after addition of 10% (w/v) excess glucose and (iii) the accumulated intracellular ethanol is released when aerobic fermentation is stimulated with decreasing glucose concentration.


CONCLUSIONS
Intracellular ethanol accumulation occurs in initial stage of a rapid aerobic fermentation and high glucose concentration may attribute to this accumulation process.


CONCLUSIONS
This work demonstrates LTRS is a real-time, reagent-free, in situ technique and a powerful tool to study kinetic process of ethanol fermentation. This work also provides further information on the intracellular ethanol accumulation in yeast cells.
Biophysics Laboratory, Guangxi Academy of Sciences, Nanning, Guangxi, China.
L
L
Peng
G
G
Wang
W
W
Liao
H
H
Yao
S
S
Huang
Y-Q
YQ
Li
D000332:Aerobiosis; D003470:Culture Media / Q000737:chemistry; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D052898:Optical Tweezers; D012441:Saccharomyces cerevisiae / Q000254:growth & development / Q000378:metabolism*; D013059:Spectrum Analysis, Raman / Q000379:methods*
D003470:Culture Media; D000431:Ethanol; D005947:Glucose