In vivo cytogenetic studies on rat's bone-marrow cells of structurally related Schiff base complexes.
The in vivo interactions of structurally-related Ni(II) and Fe(III) Schiff base complexes based on N-(8-quinolyl)salicylaldimine (HL(1)) and N-(8-quinolyl)napthaldimine (HL(2)) ligands with DNA molecules in the bone-marrow cells of rats were demonstrated using chromosomal aberrations (CAs) assay. The complexes differ by one aromatic group on the aldehyde site of the Schiff base (basicity or lipophilicity), or by the type of the central metal ions (Ni(II) or Fe(III)). Animals were injected intraperitoneally (i.p) with different concentrations of each drug, and CAs were examined in bone-marrow cells, 15 hours later. A significant increase in the frequency of CAs was induced upon treatment with 15 mg / kg weight of L(1) complexes (P < 0.001), and not with L(2) complexes (P > 0.05). Also, the magnitude of aberrations induced by L(1)-Ni(II) was higher than that induced by L(1)-Fe(III) (P < 0.01). The binding data, estimated using UV-Visible absorption technique, showed that the metal binding of HL(1) was much greater than that of HL(2) and that the affinity of HL(1) towards Ni(II) is higher than that for Fe(III) ions. Thus, the trends in the presented in vivo results signify the important role of complex stability in predicting the clastogenicity of metal-ion-chelating Schiff base drugs.
Department of Chemistry, College of Science, UAE University, Al-Ain, UAE. n.saleh@uaeu.ac.ae
Na'il
N
Saleh
Omar F
OF
Khabour
Fatima T
FT
Esmadi
Etizaz
E
Al-Kofahi
D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects* / Q000473:pathology; D002869:Chromosome Aberrations / Q000139:chemically induced*; D004305:Dose-Response Relationship, Drug; D007274:Injections, Intraperitoneal; D008024:Ligands; D008297:Male; D008940:Mitotic Index; D015394:Molecular Structure; D009152:Mutagenicity Tests; D051381:Rats; D017208:Rats, Wistar; D012545:Schiff Bases / Q000737:chemistry / Q000633:toxicity*; D013329:Structure-Activity Relationship
D008024:Ligands; D012545:Schiff Bases