Optimization of silica surface with nanosize holes for immobilization of biomolecules and analysis of their interactions.
An evanescent-field-coupled waveguide-mode sensor of the Kretschmann configuration with a silica waveguide having nanoscale holes is an ideal tool for detection of bimolecular reactions. In the present research, an optimized surface of the sensor with cylindrical nanoscale holes was modified with sodium (1-{[6-(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)hexanoyl]oxy}-2,5-dioxopyrrolidine-3-sulfonate) (Sulfo-EMCS) to facilitate the attachment of biomolecules; the resulting surface could be cleaned for reuse simply by changing the pH of the buffering solution. The modification is expected to be useful for wide range of molecular detection.
Photonics Research Institute, Bio-Photonics group, National Institute of Advanced Industrial Science and Technology, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8562, Japan. gopi-subashchandrabose@aist.go.jp
Subash C B
SC
Gopinath
Koichi
K
Awazu
Makoto
M
Fujimaki
Penmetcha K R
PK
Kumar
Tetsuro
T
Komatsubara
D015374:Biosensing Techniques; D005164:Factor IX / Q000032:analysis* / Q000096:biosynthesis*; D008855:Microscopy, Electron, Scanning; D036103:Nanotechnology; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry*; D013499:Surface Properties
D012822:Silicon Dioxide; D005164:Factor IX