The time domain in co-stained cell imaging: time-resolved emission imaging microscopy using a protonatable luminescent iridium complex.
The intense luminescence of the new complex Ir(ppy)(2)(pybz) (1) within the cytoplasm of live cells can be discriminated from the fluorescence of an organic stain, solely on the basis of the emission timescale {pybzH = 2-pyridyl-benzimidazole}. The protonated form of 1 displays red-shifted emission, and may be implicated in a superior uptake compared to Ir(ppy)(3).
Murphy
Lisa
L
Department of Chemistry, University of Durham, Durham, UK DH1 3LE.
Congreve
Aileen
A
Pålsson
Lars-Olof
LO
Williams
J A Gareth
JA
D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D007495:Iridium / Q000737:chemistry*; D049449:Luminescence; D008856:Microscopy, Fluorescence; D008968:Molecular Conformation; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D013997:Time Factors
D009942:Organometallic Compounds; D007495:Iridium