Species-diagnostic and species-specific DNA sequences evenly distributed throughout pine and spruce chromosomes.
Genome organization in the family Pinaceae is complex and largely unknown. The main purpose of the present study was to develop and physically map species-diagnostic and species-specific molecular markers in pine and spruce. Five RAPD (random amplified polymorphic DNA) and one ISSR (inter-simple sequence repeat) species-diagnostic or species-specific markers for Picea mariana, Picea rubens, Pinus strobus, or Pinus monticola were identified, cloned, and sequenced. In situ hybridization of these sequences to spruce and pine chromosomes showed the sequences to be present in high copy number and evenly distributed throughout the genome. The analysis of centromeric and telomeric regions revealed the absence of significant clustering of species-diagnostic and species-specific sequences in all the chromosomes of the four species studied. Both RAPD and ISSR markers showed similar patterns.
Biomolecular Sciences, Department of Biology, Laurentian University, Sudbury, ON, Canada.
Melanie
M
Mehes-Smith
Paul
P
Michael
Kabwe
K
Nkongolo
D001483:Base Sequence; D002678:Chimera / Q000145:classification / Q000235:genetics; D002874:Chromosome Mapping / Q000379:methods; D032461:Chromosomes, Plant / Q000235:genetics; D018744:DNA, Plant / Q000032:analysis / Q000235:genetics; D005819:Genetic Markers / Q000502:physiology; D018895:Microsatellite Repeats / Q000235:genetics; D008969:Molecular Sequence Data; D058889:Molecular Typing / Q000379:methods*; D028222:Picea / Q000145:classification / Q000235:genetics*; D028223:Pinus / Q000145:classification / Q000235:genetics*; D019105:Random Amplified Polymorphic DNA Technique; D017422:Sequence Analysis, DNA; D013045:Species Specificity
D018744:DNA, Plant; D005819:Genetic Markers