High-throughput SuperSAGE.
SuperSAGE is a method of digital gene expression profiling that allows isolation of 26-bp tag fragments from expressed transcripts. Combined with the ultrahigh-throughput sequencing technologies, SuperSAGE enables analysis of millions of transcripts with lower cost and reduced effort and time. In this chapter, we present an updated protocol for this High-throughput SuperSAGE method with a special emphasis on the technique of library multiplexing.
Matsumura
Hideo
H
Iwate Biotechnology Research Center, Kitakami, Iwate, Japan.
Yoshida
Kentaro
K
Luo
Shujun
S
Krüger
Detlev H
DH
Kahl
Günter
G
Schroth
Gary P
GP
Terauchi
Ryohei
R
D001483:Base Sequence; D017931:DNA Primers; D020869:Gene Expression Profiling; D017422:Sequence Analysis, DNA / Q000379:methods*
D017931:DNA Primers