Interaction of CO dehydrogenase with the cytoplasmic membrane monitored by fluorescence correlation spectroscopy.
We have investigated the interaction of carbon monoxide dehydrogenase (CODH), an enzyme that catalyses the oxidation of CO in the aerobic eubacterium Oligotropha carboxidovorans, with the cytoplasmic membrane by using fluorescence correlation spectroscopy (FCS). Our results reveal that in vitro this interaction of CODH is specific for cytoplasmic membranes from CO-grown bacteria.
Experimentalphysik IV and Forschungszentrum für Bio-Makromoleküle, Universität Bayreuth, Universitätsstrasse 30, 95447 Bayreuth, Germany.
Florian
F
Spreitler
Christian
C
Brock
Astrid
A
Pelzmann
Ortwin
O
Meyer
Jürgen
J
Köhler
D000445:Aldehyde Oxidoreductases / Q000737:chemistry / Q000378:metabolism*; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism*; D041841:Bradyrhizobiaceae / Q000254:growth & development / Q000378:metabolism; D002248:Carbon Monoxide / Q000378:metabolism; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D020285:Cryoelectron Microscopy; D005452:Fluoresceins / Q000737:chemistry / Q000378:metabolism; D008081:Liposomes / Q000737:chemistry / Q000378:metabolism; D018613:Microscopy, Confocal; D046529:Microscopy, Electron, Transmission; D009097:Multienzyme Complexes / Q000737:chemistry / Q000378:metabolism*; D010084:Oxidation-Reduction; D011485:Protein Binding; D013050:Spectrometry, Fluorescence / Q000379:methods*
D001426:Bacterial Proteins; D005452:Fluoresceins; D008081:Liposomes; D009097:Multienzyme Complexes; D002248:Carbon Monoxide; D000445:Aldehyde Oxidoreductases; C019823:carbon monoxide dehydrogenase