[The nude mice model of human cryopreserved umbilical cord blood transplantation].
BACKGROUND
To evaluate the hematopoietic potential of long-term cryopreserved human umbilical cord blood as well as the migration of the stem cells in nude mice.


METHODS
Thirty lethally irradiated BALB/ C nu + mice were randomized into 3 groups. One group was injected cryopreserved human umbilical cord blood cells intravenously and one group was injected fresh cord blood and the other one was injected normal saline as control. The survival rates of mice and the successful transplantation evidence were observed and compared among three groups. The migration of the stem cells was analyzed by PCR and fluid cytometry.


RESULTS
The survival rate of the cryopreserved group was much higher than that of the saline group , but there was no significant difference between the fresh group and the cryopreserved group. The evidence of human hematopoiesis was observed by PCR and fluid cytometry and lasted for at least 130 days. In addition , the migration of the cryopreserved stem cells was not the same as that of fresh cord blood , where human gene was hardly detected in liver and kidneys.


CONCLUSIONS
Cryopreservation can keep the hematopoietic potential of cord blood for a long time , but may influence the adhesion molecular of the stem cells , hence influence the homing behavior of the stem cells.
The First Affiliated Hospital of Wenzhou Medical College , Wenzhou , Zhejiang 325000 , P. R. China.
H
H
Liu
J
J
Lan
J
J
Chen
Q
Q
Chen
X
X
Chen
Q
Q
Sun
M
M
Gan