Studying RNA-RNA and RNA-protein interactions by isothermal titration calorimetry.
Isothermal Titration Calorimetry (ITC) provides a sensitive and accurate means by which to study the thermodynamics of RNA folding, RNA binding to small molecules, and RNA-protein interactions. The advent of extremely sensitive instrumentation and the increasing availability of ITC in shared facilities have made it increasingly valuable as a tool for RNA biochemistry. As an isothermal measurement, it allows analysis at a defined temperature, distinguishing it from thermal melting approaches (UV melting and differential scanning calorimetry, for instance) that provide thermodynamic information specific to the melting temperature. Residual structures at low temperature in the unfolded state and heat capacity changes lead to potential differences between thermodynamic values measured by ITC and those derived from melting studies. This article describes how ITC can be put to use in the study of RNA biochemistry.
Feig
Andrew L
AL
Department of Chemistry, Wayne State University, Detroit, Michigan, USA.
D002151:Calorimetry / Q000379:methods*; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry*; D012313:RNA / Q000737:chemistry*
D011506:Proteins; D012313:RNA