Chicken chemokine receptors in T cells isolated from lymphoid organs and in splenocytes cultured with concanavalin A.
Chemokine receptors guide immune cells to specific organs during health and disease. The mRNA content of the chemokine receptors CCR2, CCR4, CCR5, CCR6, CCR7, CCR8, CCR9, CXCR4, CXCR5, and CX3CR1 in CD4(+) cells (T-helper cells) isolated from blood, bursa, cecal tonsil, spleen, and thymus and in CD8(+) cells (T-cytotoxic cells) isolated from blood, cecal tonsil, spleen, and thymus were investigated. The CD4(+) cells isolated from thymus had the highest amount of CCR7 and CCR8 mRNA. The CD4(+) cells isolated from bursa, cecal tonsil, and thymus had the highest amount of CCR5 mRNA. The CD4(+) cells isolated from cecal tonsils had the highest amount of CCR9 mRNA. The CD4(+) cells isolated from bursa and thymus had the highest amount of CXCR5 mRNA. The CD8(+) cells isolated from cecal tonsil had the highest mRNA amount of all receptors studied except CCR9 and CX3CR1. The CD4(+) cells treated with concanavalin A had increased CCR2, CCR4, CCR7, CCR8, and CXCR5 mRNA amounts at 24 h of stimulation. The CD8(+) cells treated with concanavalin A had increased CCR4 mRNA at 72 h, increased CCR6 mRNA at 24 h, and decreased CCR8 and CXCR4 mRNA at 24 h of stimulation.
Department of Animal Sciences, Ohio Agricultural Research and Development Center, Wooster 44691, USA.
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Annamalai
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Selvaraj
D000818:Animals; D002060:Bursa of Fabricius / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D002432:Cecum / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D002645:Chickens / Q000276:immunology*; D003208:Concanavalin A / Q000494:pharmacology; D017931:DNA Primers; D014066:Palatine Tonsil / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D016133:Polymerase Chain Reaction / Q000379:methods; D019707:Receptors, Chemokine / Q000235:genetics / Q000276:immunology*; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D013950:Thymus Gland / Q000166:cytology / Q000187:drug effects / Q000276:immunology
D017931:DNA Primers; D019707:Receptors, Chemokine; D003208:Concanavalin A