Induction of heat shock proteins in the adult rat cerebral cortex following pilocarpine-induced status epilepticus.
The expression pattern of a global set of Hsps in the adult rat cerebral cortex was examined during the first week following pilocarpine-induced status epilepticus (SE). Hsp70 and Hsp32 protein were induced at 1 day post-SE and decreased thereafter, whereas Hsp25 exhibited a prolonged induction with increased levels at 3 and 7 days post-SE. Hsp70 was induced in neurons, while Hsp25 and Hsp32 localized to GFAP-positive hypertrophied astrocytes. The induction of Hsp25, Hsp32 and Hsp70 at 1 day following SE suggests the involvement of these Hsps in early events after SE. Interestingly, while Hsp32 and Hsp70 expression was transient, Hsp25 demonstrated a sustained induction pattern, which may reflect an additional role of Hsp25 in subsequent remodeling events in the days following SE.
Center for the Neurobiology of Stress, University of Toronto Scarborough, Toronto, Ontario, Canada.
Starlee
S
Lively
Ian R
IR
Brown
D000818:Animals; D001253:Astrocytes / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism*; D055551:HSP27 Heat-Shock Proteins / Q000378:metabolism*; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D006419:Heme Oxygenase (Decyclizing) / Q000378:metabolism*; D008297:Male; D009474:Neurons / Q000378:metabolism; D010862:Pilocarpine; D051381:Rats; D017208:Rats, Wistar; D013226:Status Epilepticus / Q000139:chemically induced / Q000378:metabolism*; D013997:Time Factors
D055551:HSP27 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; C486114:Hspb1 protein, rat; D010862:Pilocarpine; D006419:Heme Oxygenase (Decyclizing); C491523:Hmox1 protein, rat