Role of bottom-fermenting brewer's yeast KEX2 in high temperature resistance and poor proliferation at low temperatures.
Variants of bottom-fermenting brewer's yeast that grew at high temperatures and showed poor proliferation and fermentation at low temperatures were isolated. Similar variants of laboratory yeast were also isolated and found to be incapable of mating. The KEX2 gene was cloned by complementation. It was shown to be responsible for these traits, because a KEX2 disruptant of Saccharomyces cerevisiae (S. cerevisiae) laboratory yeast grew poorly at low temperatures and was resistant to high temperatures. In addition, a Saccharomyces bayanus (S. bayanus)-type KEX2 (Sb-KEX2) disruptant of bottom-fermenting brewer's yeast grew poorly at low temperatures and was resistant to high temperatures. The KEX2 gene product plays an important role in proliferation of yeast at low temperatures, which is an important trait of bottom-fermenting brewer's yeast. These findings advance our understanding of the proliferation of yeast at low temperatures, especially that of bottom-fermenting brewer's yeast.
Yamagishi
Hiromi
H
Research Laboratories of Brewing Technology, Asahi Breweries, LTD., Moriya, Ibaraki, Japan. hiromi.yamagishi@asahibeer.co.jp
Ohnuki
Shinsuke
S
Nogami
Satoru
S
Ogata
Tomoo
T
Ohya
Yoshikazu
Y
D003080:Cold Temperature; D005285:Fermentation; D006358:Hot Temperature; D043484:Proprotein Convertases / Q000235:genetics / Q000502:physiology*; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000502:physiology*
D029701:Saccharomyces cerevisiae Proteins; D043484:Proprotein Convertases; C058751:KEX2 protein, S cerevisiae