The mechanism of myocardium and pancreas injury in rabbits with acute renal failure might be related to myeloperoxidase and membrane pump activities.
There is increasing evidence indicating that the distant organ injury is a major contributor of high mortality in patients subjected to acute renal failure (ARF). However, sources and mechanisms that ARF causes distant organ injury remain to be determined. The aim of this study is to explore the mechanism from polymorphonuclear neutrophil (PMN) sequestration and membrane pump suppression. To achieve this, we examined myeloperoxidase (MPO), a marker of PMN accumulation in tissues, and membrane pump activities of heart, pancreas, and kidney in two ARF rabbit models. Rabbits are randomly assigned to control, HgCl(2)-treated, and glycerin-treated groups. ARF animal models are established by hypodermic injection of 1% HgCl(2) with 1.3 mL/kg bodyweight (bw) in HgCl(2)-treated group or intramuscular injection of 50% glycerin with 10 mL/kg bw in glycerin-treated group, respectively, and all animals in each group are further divided into 12 h, 24 h, and 48 h subgroups with each consisting of six rabbits. Six healthy rabbits serve as control group. Results have shown that MPO activities of kidney, myocardium, and pancreas in two model groups were significantly increased than control group at diverse time points. Membrane pump activities of kidney in two model groups are significantly lower than the control group at multiple time points. Moreover, Na(+)-K(+)-, Ca(2+)-, Mg(2+)-, and Ca(2+)-Mg(2+)-ATPase activities of myocardium and pancreas in two model groups are gradually declined with the development of ARF. These findings suggest that PMN sequestration and membrane pump suppression plays an important role in the pathogenesis of ARF and also a major mechanism of myocardium and pancreas injury during the process of ARF.
Institute of Microcirculation, Hebei North University, Zhangjiakou, Hebei, PR China.
Zi-Gang
ZG
Zhao
Chun-Yu
CY
Niu
Yu-Ping
YP
Zhang
Rui
R
Han
Ya-Li
YL
Hou
Bao-liang
BL
Li
Shu-ting
ST
Du
D058186:Acute Kidney Injury / Q000150:complications / Q000378:metabolism / Q000503:physiopathology*; D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D004195:Disease Models, Animal; D007668:Kidney / Q000378:metabolism; D009102:Multiple Organ Failure / Q000209:etiology / Q000503:physiopathology*; D009206:Myocardium / Q000378:metabolism; D009504:Neutrophils / Q000378:metabolism; D010179:Pancreas / Q000378:metabolism; D009195:Peroxidase / Q000032:analysis; D011817:Rabbits
D009195:Peroxidase; D000251:Adenosine Triphosphatases