Genetic diversity of tomato-infecting Tomato yellow leaf curl virus (TYLCV) isolates in Korea.
Epidemic outbreaks of Tomato yellow leaf curl virus (TYLCV) diseases occurred in greenhouse grown tomato (Solanum lycopersicum) plants of Busan (TYLCV-Bus), Boseong (TYLCV-Bos), Hwaseong (TYLCV-Hwas), Jeju Island (TYLCV-Jeju), and Nonsan (TYLCV-Nons) in Korea during 2008-2009. Tomato disease by TYLCV has never occurred in Korea before. We synthesized the full-length genomes of each TYLCV isolate from the tomato plants collected at each area and determined their nucleotides (nt) sequences and deduced the amino acids of six open reading frames in the genomes. TYLCV-Bus and -Bos genomes shared higher nt identities with four Japanese isolates -Ng, -Omu, -Mis, and -Miy. On the other hand, TYLCV-Hwas, -Jeju, and -Nons genomes shared higher nt identities with five Chinese isolates TYLCV-AH1, -ZJ3, -ZJHZ12, -SH2, -Sh10, and two Japanese isolates -Han and -Tosa. On the basis of a neighbor-joining tree, five Korean TYLCV isolates were separated into three clades. TYLCV-Bus and -Bos formed the first clade, clustering with four Japanese isolates TYLCV-Mis, -Omu, -Ng, and -Miy. TYLCV-Jeju and -Nons formed the second clade, clustering with two Chinese isolates -ZJHZ212 and -Sh10. TYLCV-Hwas was clustered with two Japanese isolates -Han and -Tosa and three Chinese isolates -AH1, -ZJ3, and -SH2. Two fragments that had a potentially recombinant origin were identified using the RDP, GENECONV, BootScan, MaxChi, Chimaera, SiScan, and 3Seq methods implemented in RDP3.41. On the basis of RDP analysis, all TYLCV isolates could originated from the interspecies recombination between TYLCV-Mld[PT] isolated from Portugal as a major parent and TYLCTHV-MM isolated from Myanmar as a minor parent.
Kim
Sue Hoon
SH
Department of Biology and Medicinal Science, Pai Chai University, Daejeon, Korea.
Oh
Sung
S
Oh
Tae-Kyun
TK
Park
Jae Sung
JS
Kim
Sei Chang
SC
Kim
Seong Hwan
SH
Kim
Young Shik
YS
Hong
Jeum Kyu
JK
Sim
Sang-Yun
SY
Park
Kwon Seo
KS
Lee
Hwan Gu
HG
Kim
Kyung Jae
KJ
Choi
Chang Won
CW
D000818:Animals; D053839:Begomovirus / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics*; D022761:Gene Transfer, Horizontal; D014644:Genetic Variation; D016679:Genome, Viral; D006430:Hemiptera / Q000821:virology; D007303:Insect Vectors / Q000821:virology; D018551:Solanum lycopersicum / Q000821:virology; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology; D018515:Plant Leaves / Q000821:virology; D056910:Republic of Korea; D017422:Sequence Analysis, DNA
D004279:DNA, Viral