Necl-5/PVR enhances PDGF-induced attraction of growing microtubules to the plasma membrane of the leading edge of moving NIH3T3 cells.
Microtubules (MTs) search for and grow toward the leading edge of moving cells, followed by their stabilization at a specific structure at the rear site of the leading edge. This dynamic re-orientation of MTs is critical to directional cell movement. We previously showed that Necl-5/poliovirus receptor (PVR) interacts with platelet-derived growth factor (PDGF) receptor and integrin α(v) β(3) at the leading edge of moving NIH3T3 cells, resulting in an enhancement of their directional movement. We studied here the role of Necl-5 in the PDGF-induced attraction of growing MTs to the leading edge of NIH3T3 cells. Necl-5 enhanced the PDGF-induced growth of MTs and attracted them near to the plasma membrane of the leading edge of NIH3T3 cells in an integrin α(v) β(3) -dependent manner. Furthermore, Necl-5 enhanced the PDGF-induced attraction of the plus-end-tracking proteins (+TIPs), including EB1, CLIP170, an intermediate chain subunit of cytoplasmic dynein, and p150(Glued) , a subunit of dynactin, near to the plasma membrane of the leading edge. Thus, Necl-5 plays a role in the attraction of growing MTs to the plasma membrane of the leading edge of moving cells.
Division of Molecular and Cellular Biology, Department of Biochemistry and Molecular Biology, Kobe University Graduate School of Medicine/Faculty of Medicine, Kobe, Hyogo, Japan.
Akihiro
A
Minami
Kiyohito
K
Mizutani
Masazumi
M
Waseda
Mihoko
M
Kajita
Muneaki
M
Miyata
Wataru
W
Ikeda
Yoshimi
Y
Takai
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism*; D002465:Cell Movement / Q000187:drug effects*; D039302:Integrin alphaVbeta3 / Q000378:metabolism; D051379:Mice; D008870:Microtubules / Q000378:metabolism*; D041681:NIH 3T3 Cells; D009363:Neoplasm Proteins / Q000378:metabolism; D010982:Platelet-Derived Growth Factor / Q000378:metabolism / Q000494:pharmacology*; D011485:Protein Binding; D011991:Receptors, Virus / Q000378:metabolism*
D039302:Integrin alphaVbeta3; D009363:Neoplasm Proteins; D010982:Platelet-Derived Growth Factor; D011991:Receptors, Virus; C066100:poliovirus receptor