Purification and crystallization of oxygen-evolving photosystem II core complex from thermophilic cyanobacteria.
This chapter describes the purification and crystallization of oxygen-evolving photosystem II core dimer complex from a thermophilic cyanobacterium Thermosynechococcus vulcanus. Procedures used for purification of photosystem II from the cyanobacterium involves cultivation of cells, isolation of thylakoid membranes, purification of crude and pure photosystem II core complexes by detergent solubilization, followed by differential centrifugation and column chromatography. The purified core dimer particles were successfully used for crystallization, and the methods and conditions used for crystallization are presented. These purification and crystallization procedures can be applied for another thermophilic cyanobacterium T. elongatus.
Graduate School of Natural Science and Technology, Okayama University, Okayama, Japan.
Jian-Ren
JR
Shen
Keisuke
K
Kawakami
Hiroyuki
H
Koike
D018929:Cell Culture Techniques; D005591:Chemical Fractionation / Q000379:methods*; D002852:Chromatography, Ion Exchange; D003460:Crystallization / Q000379:methods*; D000458:Cyanobacteria / Q000166:cytology / Q000201:enzymology*; D010100:Oxygen / Q000378:metabolism*; D045332:Photosystem II Protein Complex / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D055503:Protein Multimerization; D020836:Protein Structure, Quaternary; D020524:Thylakoids / Q000201:enzymology
D045332:Photosystem II Protein Complex; D010100:Oxygen