Surface IgM on DT40 cells may be a component of the putative receptor complex responsible for the binding of infectious bursal disease virus.
To investigate the host-pathogen interactions between infectious bursal disease virus (IBDV) and target B-lymphocytic cells, a cDNA T7 phage display library from the chicken bursa of Fabricius was constructed and screened for virus binding. Surface immunoglobulin M (sIgM) was isolated as a putative candidate binding site and its interactions with IBDV were further investigated using a chicken bursal lymphoma-derived cell line DT40. The results showed that the λ light chain of sIgM specifically interacted with IBDV in a virulence-independent manner in vitro, and most of the binding of IBDV to DT40 cells was inhibited by sIgM-specific monoclonal antibodies. Further, the infectivity of IBDV in vitro was reduced by sIgM-specific monoclonal antibodies. Our data provided evidence that sIgM may participate as one of the putative membrane binding sites responsible for IBDV infection.
Luo
Jun
J
Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, China.
Zhang
Hong
H
Teng
Man
M
Fan
Jian-Ming
JM
You
Lei-Ming
LM
Xiao
Zhi-Jun
ZJ
Yi
Ming-Lin
ML
Zhi
Yu-Bao
YB
Li
Xue-Wu
XW
Zhang
Gai-Ping
GP
D000818:Animals; D045744:Cell Line, Tumor; D002645:Chickens; D005786:Gene Expression Regulation; D007075:Immunoglobulin M / Q000378:metabolism*; D007243:Infectious bursal disease virus / Q000276:immunology*; D008214:Lymphocytes / Q000378:metabolism*; D008565:Membrane Proteins / Q000276:immunology / Q000378:metabolism*; D019151:Peptide Library; D011485:Protein Binding
D007075:Immunoglobulin M; D008565:Membrane Proteins; D019151:Peptide Library