An efficient and high-throughput electroporation microchip applicable for siRNA delivery.
Here we report a novel electroporation microchip with great performance and compatibility with the standard multi-well plate used in biological research. The novel annular interdigitated electrode design makes it possible to achieve efficient cell transfection as high as 90% under low-strength electrical pulses, thereby circumventing the many adverse effects of conventional cuvette-type and previously reported microchip-based electroporation devices. Using this system, we demonstrated substantially improved cell transfection efficacy and viability in cultured and primary cells, for both plasmid and synthetic siRNA. Improvements of this system open new opportunities for high-throughput applications of siRNA technology in basic and biomedical research.
Huang
Huang
H
Institute of Molecular Medicine, Peking University, Beijing, 100871, China.
Wei
Zewen
Z
Huang
Yuanyu
Y
Zhao
Deyao
D
Zheng
Lianghong
L
Cai
Tianjing
T
Wu
Mengxi
M
Wang
Wei
W
Ding
Xianfeng
X
Zhou
Zhuan
Z
Du
Quan
Q
Li
Zhihong
Z
Liang
Zicai
Z
D000818:Animals; D002460:Cell Line; D002470:Cell Survival; D002478:Cells, Cultured; D018274:Electroporation / Q000191:economics / Q000295:instrumentation*; D004867:Equipment Design; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000191:economics / Q000295:instrumentation*; D034741:RNA, Small Interfering / Q000008:administration & dosage*; D014162:Transfection / Q000191:economics / Q000295:instrumentation*
D034741:RNA, Small Interfering