Immunogenicity of a DNA vaccine of Avian Reovirus orally delivered by attenuated Salmonella typhimurium.
This study aimed to assess the ability of a Salmonella typhimurium-mediated Avain Reovirus DNA vaccine in eliciting antibody production. Six-day-old SPF chickens were orally immunized with SL7207 (pVAX-σC) twice at 2-week interval, detectable antibody was generated 2 weeks after immunization and was significantly higher than the control groups (P<0.01) and ten chickens (66.7%) were considered safe in the subsequent challenge. These results show that SL7207 (pVAX-σC) can induce protective antibody in chickens and the newly-constructed vaccine is also effective in protection chickens against ARV infection.
Wan
Junjie
J
Laboratory of Animal Infectious Disease and Microarray, Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China.
Wang
Cunwei
C
Wen
Xintian
X
Huang
Xiaobo
X
Ling
ShanShan
S
Huang
Yong
Y
Cao
Sanjie
S
D000284:Administration, Oral; D000818:Animals; D002645:Chickens; D030101:Orthoreovirus, Avian / Q000276:immunology*; D011201:Poultry Diseases / Q000517:prevention & control* / Q000821:virology; D012088:Reoviridae Infections / Q000517:prevention & control / Q000662:veterinary*; D012486:Salmonella typhimurium / Q000235:genetics*; D013047:Specific Pathogen-Free Organisms; D014613:Vaccines, Attenuated / Q000276:immunology; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology*
D014613:Vaccines, Attenuated; D019444:Vaccines, DNA; D014765:Viral Vaccines