Nasal mucosa study of leprosy contacts with positive serology for the phenolic glycolipid 1 antigen.
Leprosy is a chronic infectious disease caused by Mycobacterium leprae. The disease more frequently affects the nasal mucosa and can occur independently of its clinical form or even before lesions on the skin or on other parts of the body. It is necessary to employ epidemiological surveillance of household contacts with new leprosy cases for early disease diagnosis.


OBJECTIVE
identify specific and early leprosy lesions through endoscopic, baciloscopy, histopathology exams, and real time polymerase chain reaction of the nasal cavity mucosa on household and peridomiciliary contacts with positive serology for the phenolic glycolipid 1 antigen.


METHODS
Between 2003 at 2006 there was a prospective cross-sectional clinical study with 31 contacts with patients with leprosy with positive serology against PGL-1, 05 negative controls and 01 positive control.


RESULTS
Between seropositive contacts, real-time PCR was positive for M. leprae DNA in 06 (19.35%) of them and the higher number of genome copies were found in contacts who became sick.


CONCLUSIONS
Nasal mucosa tests alone did not enable the early diagnosis of Leprosy. However, through the combination of various methods, tests on the contacts can help identify subclinical infection and monitor the contacts that could be responsible for spreading the disease.
Fundação Oswaldo Cruz/FIOCRUZ- RJ, Brasil.
Ana Cristina da Costa
AC
Martins
Alice
A
Miranda
Maria Leide Wan-del-Rey de
ML
Oliveira
Samira
S
Bührer-Sékula
Alejandra
A
Martinez
D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D000942:Antigens, Bacterial / Q000032:analysis / Q000276:immunology*; D003430:Cross-Sectional Studies; D005260:Female; D006017:Glycolipids / Q000032:analysis / Q000276:immunology*; D006801:Humans; D007918:Leprosy / Q000175:diagnosis* / Q000382:microbiology / Q000635:transmission; D008297:Male; D008875:Middle Aged; D009166:Mycobacterium leprae / Q000235:genetics / Q000276:immunology*; D009297:Nasal Mucosa / Q000276:immunology* / Q000382:microbiology; D016133:Polymerase Chain Reaction; D011446:Prospective Studies; D012220:Rhinitis / Q000145:classification / Q000175:diagnosis; D012737:Sex Factors; D055815:Young Adult
D000942:Antigens, Bacterial; D006017:Glycolipids; C045169:phenolic glycolipid I, Mycobacterium leprae