Identification of zRAP55,a gene preponderantly expressed in Stages I and II oocytes of zebrafish.
In an in silico search for gonand specific expressed genes, we have identified zRAP55 which is enriched in the ovary of zebrafish . zRAP55 encodes a protein of 382 amino acids with a highly conserved Lsm domain. zRAP55 protein shares more than 56% identities with that of other vertebrate species. RT-PCR results show that it is predominantly expressed in the ovary. In situ hybridization and immunohistochemistry studies reveal that zRAP55 is ubiquitously dispersed throughout the cytoplasm of stages I and II oocytes, whereas no expression is observed in stages III and IV oocytes. As an RNA associated protein, zRAP55 might function in the control of protein translation at the early stages of oogenesis in zebrafish.
Fisheries College, Huazhong Agricultural University, Wuhan 430070, China. pingsongs@gmail.com.
Cai-Lian
CL
Zhao
Qi-Wen
QW
Yang
Jia-Rui
JR
Hu
Ding
D
Ye
Wu-Ming
WM
Gong
Hai-Ying
HY
Lu
Zong-Yun
ZY
Xu
Xun-Pu
XP
Zhang
Ping
P
Song
D000595:Amino Acid Sequence; D000818:Animals; D005260:Female; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D009865:Oocytes / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D016601:RNA-Binding Proteins / Q000032:analysis / Q000737:chemistry / Q000235:genetics*; D011817:Rabbits; D016415:Sequence Alignment; D015027:Zebrafish / Q000235:genetics*; D029961:Zebrafish Proteins / Q000032:analysis / Q000737:chemistry / Q000235:genetics*
D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D029961:Zebrafish Proteins; C556272:lsm14b protein, zebrafish