Multiple-trap laser tweezers Raman spectroscopy for simultaneous monitoring of the biological dynamics of multiple individual cells.
We report the development of a multiple-trap laser tweezers Raman spectroscopy (LTRS) array for simultaneously acquiring Raman spectra of individual cells in physiological environments. This LTRS-array technique was also combined with phase contrast and fluorescence microscopy, allowing measurement of Raman spectra, refractility, and fluorescence images of individual cells with a temporal resolution of ~5 s. As a demonstration, we used this technique to monitor multiple Bacillus cereus spores germinating in a nutrient medium for up to 90min and observed the kinetics of dipicolinic acid release and uptake of nucleic acid-binding stain molecules during spore germination.
Zhang
Pengfei
P
1Department of Physics, East Carolina University, Greenville, North Carolina 27858-4353, USA.
Kong
Lingbo
L
Setlow
Peter
P
Li
Yong-Qing
YQ
D001409:Bacillus cereus / Q000737:chemistry* / Q000502:physiology; D008856:Microscopy, Fluorescence / Q000379:methods; D052898:Optical Tweezers; D010848:Picolinic Acids / Q000032:analysis / Q000378:metabolism; D013059:Spectrum Analysis, Raman / Q000379:methods*; D013171:Spores, Bacterial / Q000737:chemistry* / Q000502:physiology; D013194:Staining and Labeling
D010848:Picolinic Acids; C004860:dipicolinic acid