NMR and MS methods for metabonomics.
Metabonomics, also often referred to as "metabolomics" or "metabolic profiling," is the systematic profiling of metabolites in bio-fluids or tissues of organisms and their temporal changes. In the last decade, metabonomics has become increasingly popular in drug development, molecular medicine, and other biotechnology fields, since it profiles directly the phenotype and changes thereof in contrast to other "-omics" technologies. The increasing popularity of metabonomics has been possible only due to the enormous development in the technology and bioinformatics fields. In particular, the analytical technologies supporting metabonomics, i.e., NMR, LC-MS, UPLC-MS, and GC-MS have evolved into sensitive and highly reproducible platforms allowing the determination of hundreds of metabolites in parallel. This chapter describes the best practices of metabonomics as seen today. All important steps of metabolic profiling in drug development and molecular medicine are described in great detail, starting from sample preparation, to determining the measurement details of all analytical platforms, and finally, to discussing the corresponding specific steps of data analysis.
Molecular Diagnostics, Novartis Pharma AG, Basel, Switzerland.
Frank
F
Dieterle
Björn
B
Riefke
Götz
G
Schlotterbeck
Alfred
A
Ross
Hans
H
Senn
Alexander
A
Amberg
D053000:Analytic Sample Preparation Methods; D000818:Animals; D002853:Chromatography, Liquid; D016208:Databases, Factual; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D020407:Internet; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D055432:Metabolomics / Q000379:methods*; D051379:Mice; D051381:Rats; D012984:Software; D013048:Specimen Handling; D013223:Statistics as Topic