Intragenomic sequence variation of the ITS-1 region within a single flow-cytometry-counted Cyclospora cayetanensis oocysts.
Cyclospora cayetanensis, a protozoan of emerging concern, causes self-limiting gastroenteritis in immune-competent hosts. It has been established that sequence variability exists in the first internal transcribed spacer region (ITS-1) of the ribosomal DNA operon from collections of oocysts obtained from individual or pooled fecal samples. To determine if single oocysts also exhibited ITS-1 sequence variability, DNA was extracted from individually flow-cytometry-counted oocysts. We determined that ITS-1 sequence variability exists at an individual-genome level for C. cayetanensis and approached or exceeded the variability exhibited among oocyst collections. ITS-1 variability, at the genome level, reduces this region's utility for inferring relationships between strains.
Pegasus Technical Services Inc., 46 East Hollister St., Cincinnati, Ohio 45219, USA.
Diana K
DK
Riner
Tonya
T
Nichols
Sasha Y
SY
Lucas
Andrew S
AS
Mullin
John H
JH
Cross
H D Alan
HD
Lindquist
D017124:Conserved Sequence; D021744:Cyclospora / Q000145:classification / Q000235:genetics*; D021866:Cyclosporiasis / Q000469:parasitology*; D016054:DNA, Protozoan / Q000737:chemistry; D021903:DNA, Ribosomal Spacer / Q000737:chemistry*; D005243:Feces / Q000469:parasitology; D005434:Flow Cytometry; D005759:Gastroenteritis / Q000469:parasitology*; D006801:Humans; D033661:Oocysts; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment
D016054:DNA, Protozoan; D021903:DNA, Ribosomal Spacer