Model-based inference of biochemical parameters and dynamic properties of microbial signal transduction networks.
Because of the inherent uncertainty about quantitative aspects of signalling networks it is of substantial interest to use computational methods that allow inferring non-measurable quantities such as rate constants, from measurable quantities such as changes in protein abundances. We argue that true biochemical parameters like rate constants can generally not be inferred using models due to their non-identifiability. Recent advances, however, facilitate the analysis of parameter identifiability of a given model and automated discrimination of candidate models, both being important techniques to still extract quantitative biological information from experimental data.
Theoretical Biophysics, Humboldt-Universität Berlin, Invalidenstrasse 42, Berlin, Germany. schaber@med.ovgu.de
Jörg
J
Schaber
Edda
E
Klipp
D000465:Algorithms; D016477:Artifacts; D019295:Computational Biology / Q000379:methods*; D003198:Computer Simulation; D007700:Kinetics; D008827:Microbiological Phenomena; D008954:Models, Biological; D056004:Molecular Dynamics Simulation; D011506:Proteins / Q000032:analysis / Q000378:metabolism; D015398:Signal Transduction
D011506:Proteins