A large Finnish echovirus 30 outbreak was preceded by silent circulation of the same genotype.
An outbreak of echovirus 30 (E-30) in 2009 was confirmed by both frequent isolation of the virus from sewage as well as from patient samples in Finland. Over the last 10 years E-30 had only been isolated sporadically in Finland. We here study the phylogenetic relationships of the strains from the outbreak in the context of E-30 circulation over the last 20 years. The analyzed region comprised 276 nucleotides in the 5' end of VP1 (nucleotides 132-407 in the VP1 of the E-30 Bastianni strain). The Finnish strains were clustered into at least four distinct genogroups, with seven clusters exceeding the genotype demarcation of 12% and the 2009 epidemic strains forming the largest genogroup VII. Moreover, we detected largely divergent genotypes in 2007 and 2009. Interestingly, close genetic relatives of the epidemic strains had already been isolated a few years before the outbreak. Phylodynamic analysis estimated 8.9 years (95% highest posterior density intervals 7.0-11.0) as the age of genogroup VII, indicating a probable origin and evolutionary history prior to its introduction and epidemic expansion in Finland. Finally, the most recent common ancestor for the current E-30 diversity dates back to 1939 (95% highest posterior density intervals 1913-1956).
Savolainen-Kopra
Carita
C
Intestinal Viruses Unit, Department of Infectious Disease Surveillance and Control, National Institute for Health and Welfare, Helsinki, Finland. carita.savolainen-kopra@thl.fi
Paananen
Anja
A
Blomqvist
Soile
S
Klemola
Päivi
P
Simonen
Marja-Leena
ML
Lappalainen
Maija
M
Vuorinen
Tytti
T
Kuusi
Markku
M
Lemey
Philippe
P
Roivainen
Merja
M
D000293:Adolescent; D000328:Adult; D036022:Capsid Proteins / Q000235:genetics; D002648:Child; D002675:Child, Preschool; D004196:Disease Outbreaks; D004457:Echovirus Infections / Q000453:epidemiology*; D029822:Enterovirus B, Human / Q000235:genetics* / Q000302:isolation & purification; D019143:Evolution, Molecular; D005243:Feces / Q000821:virology; D005260:Female; D005387:Finland / Q000453:epidemiology; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D012621:Seasons; D012722:Sewage / Q000821:virology
D036022:Capsid Proteins; D012367:RNA, Viral; D012722:Sewage