Granulosa cell ligand NPPC and its receptor NPR2 maintain meiotic arrest in mouse oocytes.
Granulosa cells of mammalian Graafian follicles maintain oocytes in meiotic arrest, which prevents their precocious maturation. We show that mouse mural granulosa cells, which line the follicle wall, express natriuretic peptide precursor type C (Nppc) messenger RNA (mRNA), whereas cumulus cells surrounding oocytes express mRNA of the NPPC receptor NPR2, a guanylyl cyclase. NPPC increased cGMP levels in cumulus cells and oocytes and inhibited meiotic resumption in vitro. Meiotic arrest was not sustained in most Graafian follicles of Nppc or Npr2 mutant mice, and meiosis resumed precociously. Oocyte-derived paracrine factors promoted cumulus cell expression of Npr2 mRNA. Therefore, the granulosa cell ligand NPPC and its receptor NPR2 in cumulus cells prevent precocious meiotic maturation, which is critical for maturation and ovulation synchrony and for normal female fertility.
Zhang
Meijia
M
State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, People's Republic of China.
Su
You-Qiang
YQ
Sugiura
Koji
K
Xia
Guoliang
G
Eppig
John J
JJ
D000818:Animals; D009320:Atrial Natriuretic Factor; D054885:Cumulus Cells / Q000378:metabolism*; D000242:Cyclic AMP / Q000378:metabolism; D006152:Cyclic GMP / Q000378:metabolism; D005260:Female; D006107:Granulosa Cells / Q000378:metabolism*; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D008024:Ligands; D008540:Meiosis; D051379:Mice; D008954:Models, Biological; D009154:Mutation; D020098:Natriuretic Peptide, C-Type / Q000235:genetics / Q000378:metabolism*; D009865:Oocytes / Q000502:physiology*; D006080:Ovarian Follicle / Q000166:cytology; D011498:Protein Precursors / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017461:Receptors, Atrial Natriuretic Factor / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D000099242:Atrial Natriuretic Factor Receptor B
D009320:Atrial Natriuretic Factor; D000242:Cyclic AMP; D006152:Cyclic GMP; D036341:Intercellular Signaling Peptides and Proteins; D008024:Ligands; D020098:Natriuretic Peptide, C-Type; D011498:Protein Precursors; D012333:RNA, Messenger; D017461:Receptors, Atrial Natriuretic Factor; D000099242:Atrial Natriuretic Factor Receptor B; C431578:Nppa protein, mouse; C553310:natriuretic peptide precursor type C, mouse