Counting touching cell nuclei using fast ellipse detection to assess in vitro cell characteristics: a feasibility study.
In this article, we describe a new image analysis software that allows rapid segmentation and separation of fluorescently stained cell nuclei using a fast ellipse detection algorithm. Detection time ranged between 1.84 and 3.14 s. Segmentation results were compared with manual evaluation. The achieved over-segmentation rate was 0.11 (0.1 double counts and 0.01 false positive detections), and the under-segmentation rate was of 0.03 over all images. We demonstrate the applicability of the proposed algorithm to automated counting of fluorescent-labeled cell nuclei and to tissue characterization. Moreover, the performance of the proposed algorithm is compared with preexisting automated image analysis techniques described by others.
Department of Oral Surgery, University Medical Center of Johannes Gutenberg University Mainz, Augustusplatz 2, 55131, Mainz, Germany. bruellmd@mail.uni-mainz.de
Dan Dominik
DD
Brüllmann
Andreas
A
Pabst
Karl M
KM
Lehmann
Thomas
T
Ziebart
Marc O
MO
Klein
Bernd
B
d'Hoedt
D000465:Algorithms; D000538:Aluminum Silicates / Q000737:chemistry; D001672:Biocompatible Materials / Q000737:chemistry; D050071:Bone Density Conservation Agents / Q000494:pharmacology; D002452:Cell Count / Q000379:methods*; D018929:Cell Culture Techniques; D002467:Cell Nucleus / Q000187:drug effects / Q000648:ultrastructure*; D049109:Cell Proliferation / Q000187:drug effects; D048429:Cell Size / Q000187:drug effects; D002516:Ceramics / Q000737:chemistry; D004164:Diphosphonates / Q000494:pharmacology; D042783:Endothelial Cells / Q000187:drug effects / Q000648:ultrastructure; D005188:False Negative Reactions; D005189:False Positive Reactions; D005240:Feasibility Studies; D005347:Fibroblasts / Q000187:drug effects / Q000648:ultrastructure; D005456:Fluorescent Dyes; D006801:Humans; D000077557:Ibandronic Acid; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008853:Microscopy; D010012:Osteogenesis / Q000187:drug effects; D000077268:Pamidronate; D017680:Potassium Compounds / Q000737:chemistry; D012984:Software; D013234:Stem Cells / Q000187:drug effects / Q000648:ultrastructure; D013997:Time Factors; D014025:Titanium / Q000737:chemistry
D000538:Aluminum Silicates; D001672:Biocompatible Materials; D050071:Bone Density Conservation Agents; D004164:Diphosphonates; D005456:Fluorescent Dyes; D017680:Potassium Compounds; C016447:feldspar; D014025:Titanium; D000077268:Pamidronate; D000077557:Ibandronic Acid