Ochrobactrum pecoris sp. nov., isolated from farm animals.
Two Gram-negative, rod-shaped, non-spore-forming strains, designated 08RB2639(T) and 08RB2781-1, were isolated from a sheep (Ovis aries) and a domestic boar (Sus scrofa domestica), respectively. By 16S rRNA gene sequencing, the isolates revealed identical sequences and were shown to belong to the Alphaproteobacteria. They exhibited 97.8 % 16S rRNA gene sequence similarity with Ochrobactrum rhizosphaerae PR17(T), O. pituitosum CCUG 50899(T), O. tritici SCII24(T) and O. haematophilum CCUG 38531(T) and 97.4 % sequence similarity with O. cytisi ESC1(T), O. anthropi LMG 3331(T) and O. lupini LUP21(T). The recA gene sequences of the two isolates showed only minor differences (99.5 % recA sequence similarity), and strain 08RB2639(T) exhibited the highest recA sequence similarity with Ochrobactrum intermedium CCUG 24694(T) (91.3 %). The quinone system was ubiquinone Q-10, with minor amounts of Q-9 and Q-11, the major polyamines were spermidine, putrescine and sym-homospermidine and the major lipids were phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and phosphatidylcholine, with moderate amounts of the Ochrobactrum-specific unidentified aminolipid AL2. The major fatty acids (>20 %) were C₁₈:₁ω7c and C₁₉:₀ cyclo ω8c. These traits were in excellent agreement with the assignment of the isolates to the genus Ochrobactrum. DNA-DNA relatedness and physiological and biochemical tests allowed genotypic and phenotypic differentiation from other members of the genus Ochrobactrum. Hence, it is concluded that the isolates represent a novel species, for which the name Ochrobactrum pecoris sp. nov. is proposed (type strain 08RB2639(T)  = DSM 23868(T)  = CCUG 60088(T)  = CCM 7822(T)).
Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany.
Peter
P
Kämpfer
Institut für Bakteriologie, Mykologie und Hygiene, Veterinärmedizinische Universität Wien, A-1210 Vienna, Austria.
Bettina
B
Huber
Institut für Bakteriologie, Mykologie und Hygiene, Veterinärmedizinische Universität Wien, A-1210 Vienna, Austria.
Hans-Jürgen
HJ
Busse
Bundeswehr Institute of Microbiology, D-80937 Munich, Germany.
Holger C
HC
Scholz
Friedrich Loeffler Institute, Institute of Bacterial Infections and Zoonoses, D-07743 Jena, Germany.
Herbert
H
Tomaso
Friedrich Loeffler Institute, Institute of Bacterial Infections and Zoonoses, D-07743 Jena, Germany.
Helmut
H
Hotzel
Friedrich Loeffler Institute, Institute of Bacterial Infections and Zoonoses, D-07743 Jena, Germany.
Falk
F
Melzer
D000818:Animals; D000829:Animals, Domestic / Q000382:microbiology; D015373:Bacterial Typing Techniques; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D042041:Ochrobactrum / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D011073:Polyamines / Q000032:analysis; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D011938:Rec A Recombinases / Q000235:genetics; D017422:Sequence Analysis, DNA; D012756:Sheep / Q000382:microbiology*; D034421:Sus scrofa / Q000382:microbiology*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010743:Phospholipids; D011073:Polyamines; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D011938:Rec A Recombinases