Simultaneous determination of three steroidal glycoalkaloids in Solanum xanthocarpum by high performance thin layer chromatography.
A new high-performance thin-layer chromatographic (HPTLC) method has been developed for the simultaneous quantitation of three bioactive steroidal glycoalkaloid (SGA) markers, solasonine (SN), solamargine (SM) and khasianine (KN) in the plant Solanum xanthocarpum. Extraction efficiency of targeted SGAs from plant matrix using methanol and acidified methanol were studied using percolation, ultrasonication and microwave techniques. The separation was achieved on silica gel 60F(254) TLC plates using chloroform-methanol-water as mobile phase. The quantitation of SGAs was carried out using the densitometric reflection/absorption mode at 520 nm after post chromatographic derivatization using Dragendorff's reagent. The method was validated for peak purity, precision, accuracy, robustness, limit of detection (LOD) and quantitation (LOQ). Method specificity was confirmed using retention factor (R(f)), Vis spectral correlation and electrospray ionization mass spectra (ESI-MS) of marker compounds in the sample track.
Shanker
Karuna
K
Analytical Chemistry Department, Central Institute of Medicinal and Aromatic Plants, Council of Scientific and Industrial Research, Lucknow 226015, India.
Gupta
Shalini
S
Srivastava
Pooja
P
Srivastava
Santosh K
SK
Singh
Subash C
SC
Gupta
Madan M
MM
D002855:Chromatography, Thin Layer; D003720:Densitometry; D013058:Mass Spectrometry; D010840:Phytosterols / Q000032:analysis*; D035261:Plant Components, Aerial; D010936:Plant Extracts / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012991:Solanaceous Alkaloids / Q000032:analysis*; D032322:Solanum; D013256:Steroids / Q000032:analysis*
D010840:Phytosterols; D010936:Plant Extracts; D012991:Solanaceous Alkaloids; D013256:Steroids; C110213:khasianine; C026609:beta-solamarine; C026608:alpha-solamargine