A novel triple adjuvant formulation promotes strong, Th1-biased immune responses and significant antigen retention at the site of injection.
Ovalbumin (OVA) was labeled with a near infra-red dye (*OVA) and formulated with the host defense peptide indolicidin (Indol), CpG oligodeoxynucleotide (ODN) 1826 (CpG) and/or poly(p-dicarboxylatophenoxy)-phosphazene (PP4). The immunogenicity of these *OVA formulations was evaluated in mice. All double and triple adjuvant combinations elicited strong antibody responses. *OVA formulated with CpG ODN in combination with indolicidin, PP4 or both induced only IFN-γ, while formulations with indolicidin and/or PP4 promoted predominantly IL-5 production. Overall, both IgG and IFN-γ production was superior when *OVA was combined with CpG/Indol/PP4. Furthermore, mice injected with *OVA formulated with CpG/Indol/PP4 contained detectable *OVA in the injection site two months post immunization. These results indicate that the CpG/Indol/PP4 combination promotes prolonged antigen retention and strong, antigen-specific Th1-biased immune responses.
Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, SK S7N 5E3, Canada.
Heather L
HL
Wilson
Jennifer
J
Kovacs-Nolan
Laura
L
Latimer
Rachelle
R
Buchanan
Susantha
S
Gomis
Lorne
L
Babiuk
Sylvia
S
van Drunen Littel-van den Hurk
D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000008:administration & dosage*; D005260:Female; D007074:Immunoglobulin G / Q000097:blood; D007279:Injections, Subcutaneous; D007371:Interferon-gamma / Q000378:metabolism; D015848:Interleukin-5 / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D009838:Oligodeoxyribonucleotides / Q000008:administration & dosage*; D009943:Organophosphorus Compounds / Q000008:administration & dosage*; D010047:Ovalbumin / Q000276:immunology*; D011108:Polymers / Q000008:administration & dosage*; D018417:Th1 Cells / Q000276:immunology*; D014611:Vaccination / Q000379:methods*
D000276:Adjuvants, Immunologic; D023181:Antimicrobial Cationic Peptides; C408982:CPG-oligonucleotide; D007074:Immunoglobulin G; D015848:Interleukin-5; D009838:Oligodeoxyribonucleotides; D009943:Organophosphorus Compounds; D011108:Polymers; C546078:poly(di(carboxylatophenoxy)phosphazene); C073143:indolicidin; D007371:Interferon-gamma; D010047:Ovalbumin