Protocols of two-dimensional difference gel electrophoresis to investigate mechanisms of toxicity.
In recent years, several global omics technologies have been increasingly used to better understand the molecular mechanisms of drug toxicity. Two-dimensional difference gel electrophoresis (2D-DIGE) is a large-scale proteomics high-resolution gel-based quantitative method widely used to detect protein expression alterations after drug treatment. The 2D-DIGE technology is based on the labeling of proteins with different fluorescent dyes, allowing the separation of different samples on the same gel with the use of an internal standard, thus reducing the complexity of spot pattern comparison and providing a reliable method applied to toxicology studies for the detection of modulated proteins in targeted organs.
Disposition, Safety and Animal Research, sanofi-aventis R&D, Vitry-sur-Seine, France. emmanuelle.com@univ-rennes1.fr
Emmanuelle
E
Com
Albrecht
A
Gruhler
Martine
M
Courcol
Jean-Charles
JC
Gautier
D000818:Animals; D005591:Chemical Fractionation; D064420:Drug-Related Side Effects and Adverse Reactions / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D020869:Gene Expression Profiling; D007091:Image Processing, Computer-Assisted; D007525:Isoelectric Focusing; D007668:Kidney / Q000737:chemistry; D008099:Liver / Q000737:chemistry; D011506:Proteins / Q000302:isolation & purification / Q000378:metabolism; D040901:Proteomics; D011786:Quality Control; D051381:Rats; D012984:Software; D013194:Staining and Labeling; D057825:Two-Dimensional Difference Gel Electrophoresis / Q000379:methods*
D011506:Proteins