New method to enhance ajmalicine production in Catharanthus roseus cell cultures based on the use of cyclodextrins.
The joint use of cyclodextrins and methyljasmonate, when accompanied by a short exposure to UV, enhanced extracellular ajmalicine accumulation to 1040 ± 26.6 mg/l in suspension cultured cells of Catharanthus roseus. The success of this strategy is due to the use of cyclodextrins, which not only induce ajmalicine biosynthesis but also promote adduct formation. This removes ajmalicine from the medium, reduces feedback inhibition and ajmalicine degradation, and allows its accumulation in the culture medium at elevated concentrations.
Almagro
L
L
Department of Plant Biology, Faculty of Biology, University of Murcia, Campus de Espinardo, 30100 Murcia, Spain.
López Perez
A J
AJ
Pedreño
M A
MA
D000085:Acetates / Q000378:metabolism; D001709:Biotechnology / Q000379:methods*; D030008:Catharanthus / Q000378:metabolism* / Q000528:radiation effects; D018929:Cell Culture Techniques / Q000379:methods; D003470:Culture Media / Q000737:chemistry; D003505:Cyclodextrins / Q000378:metabolism*; D003517:Cyclopentanes / Q000378:metabolism; D054883:Oxylipins / Q000378:metabolism; D046948:Secologanin Tryptamine Alkaloids / Q000378:metabolism*; D014466:Ultraviolet Rays
D000085:Acetates; D003470:Culture Media; D003505:Cyclodextrins; D003517:Cyclopentanes; D054883:Oxylipins; D046948:Secologanin Tryptamine Alkaloids; C005709:raubasine; C072239:methyl jasmonate