Preparation of native and recombinant light-harvesting chlorophyll-a/b complex.
Procedures to isolate native light-harvesting chlorophyll-a/b complex (LHCIIb) and to reconstitute recombinant LHCIIb are described. Separation of trimeric from monomeric forms and free pigment by sucrose density-gradient ultracentrifugation can be applied to both native and reconstituted complexes. The preparations are characterized by their pigment composition, protein pattern, and spectral properties.
Rühle
Wolfgang
W
Institut für Allgemeine Botanik, Johannes-Gutenberg-Universität, Mainz, Germany.
Paulsen
Harald
H
D005591:Chemical Fractionation / Q000379:methods*; D011232:Chemical Precipitation; D002942:Circular Dichroism; D045342:Light-Harvesting Protein Complexes / Q000737:chemistry / Q000302:isolation & purification*; D009532:Nickel / Q000737:chemistry; D018532:Pisum sativum / Q000201:enzymology; D010860:Pigments, Biological / Q000302:isolation & purification; D055503:Protein Multimerization; D020836:Protein Structure, Quaternary; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification*; D012995:Solubility; D013050:Spectrometry, Fluorescence; D020524:Thylakoids / Q000201:enzymology
D045342:Light-Harvesting Protein Complexes; D010860:Pigments, Biological; D011994:Recombinant Proteins; D009532:Nickel