Validation of microarray comparative genomic hybridization for comprehensive chromosome analysis of embryos.
OBJECTIVE
To validate and determine the best array-comparative genomic hybridization (aCGH; array-CGH) protocols for preimplantation genetic screening (PGS).


METHODS
Embryos had one cell removed as a biopsy specimen and analyzed by one of two array-CGH protocols. Abnormal embryos were reanalyzed by fluorescence in situ hybridization (FISH).


METHODS
Reference laboratory.


METHODS
Patients donating embryos or undergoing PGS.


METHODS
Embryo biopsy, array-CGH, FISH reanalysis.


METHODS
Diagnosis, no result rate and error rate.


RESULTS
Method one produced 11.2% of embryos with no results and a 9.1% error rate compared with 3% and 1.9% for method two, respectively. Thereafter, only method two was used clinically. The aneuploidy rate for cleavage-stage embryos was 63.2%, significantly increasing with maternal age. The chromosomes most involved in aneuploidy were 16, 22, 21, and 15. We report the first live births after array-CGH combined with single blastomere biopsy.


CONCLUSIONS
Array-CGH is proved to be highly robust (2.9% no results) and specific (1.9% error rate) when applied to rapid (24-hour) analysis of single cells biopsied from cleavage-stage embryos. This comprehensive chromosome analysis technique is the first to be validated by reanalyzing the same embryos with another technique (e.g., FISH). Unlike some alternative techniques for comprehensive chromosome screening, array-CGH does not require prior testing of parental DNA and thus advance planning and careful scheduling are unnecessary.
Gutiérrez-Mateo
Cristina
C
Reprogenetics, Livingston, New Jersey, USA.
Colls
Pere
P
Sánchez-García
Jorge
J
Escudero
Tomas
T
Prates
Renata
R
Ketterson
Kelly
K
Wells
Dagan
D
Munné
Santiago
S
D000328:Adult; D000782:Aneuploidy; D001706:Biopsy; D001755:Blastocyst / Q000502:physiology*; D001757:Blastomeres / Q000502:physiology; D055028:Comparative Genomic Hybridization / Q000379:methods / Q000592:standards*; D005260:Female; D005820:Genetic Testing / Q000379:methods / Q000592:standards; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008423:Maternal Age; D011247:Pregnancy; D011256:Pregnancy Outcome; D019836:Preimplantation Diagnosis / Q000379:methods / Q000592:standards*; D015203:Reproducibility of Results; D027724:Reproductive Techniques, Assisted / Q000592:standards