Determination of the number of binding sites and binding constant between diltiazem hydrochloride and human serum albumin by ultrasonic microdialysis coupled with online capillary electrophoresis electrochemiluminescence.
A simple, sensitive and selective determination of diltiazem hydrochloride (DLT) is described using capillary electrophoresis electrochemiluminescence (CE-ECL). The CE-ECL parameters that affect separation and detection were optimized. Under the optimized conditions, the linear range of DLT was from 0.02 to 100 μmol/L (r(2) = 0.9983), with the detection limit of 5.1 nmol/L (3σ). The relative standard deviations of ECL intensity and the migration time were <2% for 0.1 μmol/L and 22 μmol/L DLT (n = 11). A new technique for determining of the number of binding sites and binding constant between DLT and HSA was developed using ultrasonic microdialysis coupled with CE-ECL. The number of binding sites and binding constant were 5.9 and 6.3 × 10(4) L/mol, respectively. The time required for ultrasonic microdialysis was 10 times less than that for traditional dialysis. Compared with traditional dialysis, ultrasonic microdialysis is simple, rapid, and should be applicable to a wide range of interactions of drugs and biomacromolecules.
Deng
Biyang
B
Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, Ministry of Education of China, College of Chemistry and Chemical Engineering, Guangxi Normal University, Guilin, China. dengby16@163.com
Lu
Hua
H
Li
Linqiu
L
Shi
Aihong
A
Kang
Yanhui
Y
Xu
Quanxiu
Q
D001665:Binding Sites; D004110:Diltiazem / Q000737:chemistry* / Q000378:metabolism*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008163:Luminescent Measurements; D017551:Microdialysis / Q000295:instrumentation / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012709:Serum Albumin / Q000737:chemistry* / Q000378:metabolism*; D014465:Ultrasonics
D012709:Serum Albumin; D004110:Diltiazem