Multiple myeloma patients at peripheral blood stem cell harvest: restricted usage of TCR beta variable families.
The immune systems of multiple myeloma patients are suppressed by the disease itself, and this immunosuppression is further enhanced by standard therapies. The aim of our study was to evaluate the effects of initial chemotherapy and a peripheral blood mobilisation regimen on T-cell population diversity. Reverse transcription-polymerase chain reaction (RT-PCR) with a new set of primers, in combination with capillary electrophoresis, was established. The methodology was used to analyse the relative expression of 27 T-cell receptor beta variable gene families (BV families) in multiple myeloma patients undergoing peripheral blood stem cell harvest. We found that the overall BV family usage in these patients was restricted; the relative expression of 10 BV families was significantly depressed in patients compared to healthy donors. These findings demonstrate that the preparative regimen for autologous stem cell transplantation affects the T-cell population in terms of the restriction of its T-cell receptor diversity.
Centre for Biomedical Image Analysis, Faculty of Informatics, Masaryk University, Botanická 68a, 602 00 Brno, Czech Republic.
Lenka
L
Tesarova
Irena
I
Koutna
Zdenek
Z
Koristek
Martin
M
Klabusay
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D017931:DNA Primers / Q000235:genetics; D064420:Drug-Related Side Effects and Adverse Reactions; D019075:Electrophoresis, Capillary; D005260:Female; D015870:Gene Expression / Q000235:genetics / Q000276:immunology; D020869:Gene Expression Profiling / Q000379:methods; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009101:Multiple Myeloma / Q000175:diagnosis / Q000188:drug therapy / Q000276:immunology / Q000628:therapy*; D036102:Peripheral Blood Stem Cell Transplantation / Q000009:adverse effects*; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014182:Transplantation, Autologous / Q000009:adverse effects
D017931:DNA Primers; D016693:Receptors, Antigen, T-Cell, alpha-beta