Chemical investigation of different crude extracts from Teucrium ramosissimum leaves. Correlation with their antigenotoxic and antioxidant properties.
The effect of extracts obtained from Teucrium ramosissimum leaves on genotoxicity and SOS response induced by aflatoxin B(1) (0.5 μg/assay) as well as nitrofurantoin (5 μg/assay) was investigated in a bacterial assay system, i.e., the SOS chromotest with Escherichia coli PQ37. The T. ramosissimum tested extracts exhibited no genotoxicity either with or without the external S9 activation mixture. However, all the extracts, particularly the total oligomers flavonoids (TOF) extract significantly decreased the genotoxicity induced by aflatoxin B(1) and nitrofurantoin. Antioxidant capacity of the tested extracts was evaluated using the enzymatic (xanthine/xanthine oxidase assay) (X/XOD) and the non-enzymatic (NBT/Riboflavine assay) systems. TOF extract was the most effective one in inhibiting both xanthine oxidase activity and NBT reduction. Our findings emphasize the potential of T. ramosissimum to prevent mutations and also its antioxidant effect.
Department of Pharmacognosy, Faculty of Pharmacy, Monastir, Tunisia.
M
M
Ben Sghaier
W
W
Bhouri
A
A
Neffati
J
J
Boubaker
I
I
Skandrani
I
I
Bouhlel
S
S
Kilani
L
L
Chekir-Ghedira
K
K
Ghedira
D016604:Aflatoxin B1 / Q000633:toxicity; D016587:Antimutagenic Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects; D009153:Mutagens / Q000633:toxicity; D009582:Nitrofurantoin / Q000633:toxicity; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry*; D013014:SOS Response, Genetics; D031367:Teucrium / Q000737:chemistry*; D014969:Xanthine Oxidase / Q000037:antagonists & inhibitors
D016587:Antimutagenic Agents; D000975:Antioxidants; D009153:Mutagens; D010936:Plant Extracts; D009582:Nitrofurantoin; D016604:Aflatoxin B1; D014969:Xanthine Oxidase