[Application of semi-conducting nanocrystals (quantum dots) in immunocytochemistry].
Semi-conducting nanocrystals represent a new class of fluorescent inorganic objects which have a promising perspective for the application in biology and medicine. The aim of the current work was a determination of advantages and shortcimingss of quantum dots (QD) application in immunocytochemistry. It was shown that streptavidin-QD conjugates have more advantages (i.e. high intensity of fluorescence, photostability, wide excitation range, short and symmetric emission range) as compared to streptavidin conjugates with organic fluorochromes. This allows to recommend the use of QD in immunocytochemical studies. However, there are several disadvantages (like lower stability during long-term storage as compared to that one of organic fluorochrome conjugates, poor safety of aliquots, impossibility of long-term preservation of fluorescence of stained sections during their storage, incompatibility with several commercial mounting media) that limit the widespread application of nanocrystals in immunocytochemical studies.
Korzhevskiĭ
D É
DÉ
Kirik
O V
OV
Sukhorukova
E G
EG
Giliarov
A V
AV
D000818:Animals; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods* / Q000639:trends; D045663:Quantum Dots; D019809:Streptavidin / Q000737:chemistry
D005456:Fluorescent Dyes; D019809:Streptavidin