A reliable microinjectrode system for use in behaving monkeys.
We describe a modified system for the precise delivery of small volumes of drugs to brain sites of behaving monkeys during simultaneous single-neuron electrophysiology. The system combines a conventional microelectrode for recording single neurons and a small gauge microsyringe in a durable design. It incorporates newly available microfluidic components to achieve high-precision fluidic control. The system is inexpensive, reusable and easy to fabricate; it minimizes neural tissue damage and achieves reliable single-neuron recordings at the injection site.
Howard Hughes Medical Institute and Department of Neurobiology, Stanford University, Stanford, CA 94305, USA. behrad@stanford.edu
Behrad
B
Noudoost
Tirin
T
Moore
D000200:Action Potentials / Q000187:drug effects / Q000502:physiology; D000818:Animals; D004558:Electric Stimulation / Q000295:instrumentation / Q000379:methods; D004594:Electrophysiology / Q000295:instrumentation* / Q000379:methods*; D018755:GABA Agonists / Q000008:administration & dosage; D000882:Haplorhini; D008839:Microelectrodes; D008845:Microinjections / Q000295:instrumentation / Q000379:methods; D009118:Muscimol / Q000008:administration & dosage; D009474:Neurons / Q000187:drug effects / Q000502:physiology; D010775:Photic Stimulation / Q000379:methods; D017397:Prefrontal Cortex / Q000166:cytology; D015203:Reproducibility of Results; D018709:Statistics, Nonparametric; D014851:Wakefulness
D018755:GABA Agonists; D009118:Muscimol