Tag removal by site-specific cleavage of recombinant fusion proteins.
Where an affinity tag has served its purpose it may become desirable to remove it from the protein of interest. This chapter describes the removal of such fusion partners from the intended protein product by cleavage with site-specific endoproteases. Methods to achieve proteolytic cleavage of the fusion proteins are provided, along with techniques for optimising the yield of authentic product.
Charlton
Adam
A
Commonwealth Serum Laboratories Ltd, Parkville, VIC, Australia.
Zachariou
Michael
M
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D057056:Cysteine Proteases / Q000378:metabolism; D010447:Peptide Hydrolases / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism*; D057057:Serine Proteases / Q000378:metabolism; D013379:Substrate Specificity
D011993:Recombinant Fusion Proteins; D057056:Cysteine Proteases; D010447:Peptide Hydrolases; D057057:Serine Proteases