Time-resolved X-ray scattering and RNA folding.
Time-resolved small-angle X-ray scattering (SAXS) reports changes in the global conformation of macromolecules and is thus a valuable probe of structural transitions like RNA folding. Time-resolved SAXS has been applied to study folding of the Tetrahymena ribozyme. This chapter describes the methods that enable acquisition and analysis of time-resolved SAXS data and insights into RNA folding gained from these studies.
Pollack
Lois
L
School of Applied and Engineering Physics, Cornell University, Ithaca, New York, USA.
Doniach
Sebastian
S
D009690:Nucleic Acid Conformation; D012313:RNA / Q000737:chemistry*; D012542:Scattering, Radiation; D014965:X-Rays
D012313:RNA