Lysobacter korlensis sp. nov. and Lysobacter bugurensis sp. nov., isolated from soil.
Two Gram-reaction-negative, rod-shaped, gliding, yellow-pigmented bacterial strains, designated ZLD-17(T) and ZLD-29(T), were isolated from arid soil samples collected from Xinjiang Province, north-west China, and subjected to analysis using a polyphasic taxonomic approach. Both novel strains required 1.0-2.0 % (w/v) sea salts for optimal growth. Phylogenetic analysis based on 16S rRNA gene sequences indicated that these two strains belong to the genus Lysobacter within the class Gammaproteobacteria. Strain ZLD-17(T) showed highest 16S rRNA gene sequence similarities to Lysobacter capsici KCTC 22007(T) (96.9 %), Lysobacter spongiicola DSM 21749(T) (96.8 %) and Lysobacter koreensis KCTC 12204(T) (96.8 %), whereas strain ZLD-29(T) showed highest sequence similarities to Lysobacter niastensis DSM 18481(T) (96.0 %) and Lysobacter enzymogenes DSM 2043(T) (95.9 %). 16S rRNA gene sequence similarity between ZLD-17(T) and ZLD-29(T) was 96.1 %. The DNA G+C contents of strains ZLD-17(T) and ZLD-29(T) were 67.9 and 68.2 mol%, respectively. The major cellular fatty acids of both strains were summed feature 3 (iso-C₁₅:₀ 2-OH and/or C₁₆:₁ω7c), iso-C₁₇:₁ω9c, iso-C₁₆:₀, C₁₆:₀ and iso-C₁₁:₀ 3-OH; their predominant isoprenoid quinone was Q-8 and their major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. Based on their phenotypic characteristics, phylogenetic position as determined by 16S rRNA gene sequence analysis and chemotaxonomic data, strains ZLD-17(T) ( = CCTCC AB 207174(T)  = KCTC 23076(T)) and ZLD-29(T) ( = CCTCC AB 207175(T) = KCTC 23077(T)) represent two novel species of the genus Lysobacter, for which the names Lysobacter korlensis sp. nov. and Lysobacter bugurensis sp. nov. are proposed, respectively.
Zhang
Lei
L
College of Life Sciences, Wuhan University, Wuhan 430072, PR China.|College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
Bai
Juan
J
College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
Wang
Yang
Y
College of Life Sciences, Wuhan University, Wuhan 430072, PR China.
Wu
Gao-Lin
GL
State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, Northwest A&F University, Institute of Soil and Water Conservation, CAS & MWR, Yangling, Shaanxi 712100, PR China.
Dai
Jun
J
College of Life Sciences, Wuhan University, Wuhan 430072, PR China.
Fang
Cheng-Xiang
CX
College of Life Sciences, Wuhan University, Wuhan 430072, PR China.
D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008124:Locomotion; D053528:Lysobacter / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D008969:Molecular Sequence Data; D010743:Phospholipids; D010802:Phylogeny; D010860:Pigments, Biological / Q000378:metabolism; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012492:Salts / Q000378:metabolism; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010743:Phospholipids; D010860:Pigments, Biological; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D012492:Salts