Extraction and purification of a lectin from red kidney bean and preliminary immune function studies of the lectin and four Chinese herbal polysaccharides.
Reversed micelles were used to extract lectin from red kidney beans and factors affecting reverse micellar systems (pH value, ionic strength and extraction time) were studied. The optimal conditions were extraction at pH 4-6, back extraction at pH 9-11, ion strength at 0.15 M NaCl, extraction for 4-6 minutes and back extraction for 8 minutes. The reverse micellar system was compared with traditional extraction methods and demonstrated to be a time-saving method for the extraction of red kidney bean lectin. Mitogenic activity of the lectin was reasonably good compared with commercial phytohemagglutinin (extracted from Phaseolus vulgaris) Mitogenic properties of the lectin were enhanced when four Chinese herbal polysaccharides were applied concurrently, among which 50 μg/mL Astragalus mongholicus polysaccharides (APS) with 12.5 μg/mL red kidney bean lectin yielded the highest mitogenic activity and 100 mg/kg/bw APS with 12.5 mg/kg/bw red kidney bean lectin elevated mouse nonspecific immunity.
College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai Street, Xiangfang District, Harbin 150030, China.
Yufang
Y
Hou
Yubao
Y
Hou
Liu
L
Yanyan
Guang
G
Qin
Jichang
J
Li
D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D005591:Chemical Fractionation; D002645:Chickens; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D006384:Hemagglutination / Q000187:drug effects; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D007109:Immunity / Q000187:drug effects*; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D051379:Mice; D008823:Micelles; D008934:Mitogens / Q000494:pharmacology; D009994:Osmolar Concentration; D010835:Phytohemagglutinins / Q000276:immunology* / Q000302:isolation & purification* / Q000494:pharmacology; D011134:Polysaccharides / Q000276:immunology* / Q000302:isolation & purification / Q000494:pharmacology; D013154:Spleen / Q000166:cytology; D013997:Time Factors
D004365:Drugs, Chinese Herbal; D008823:Micelles; D008934:Mitogens; D010835:Phytohemagglutinins; D011134:Polysaccharides