Evaluation of a new chromogenic medium (StrepB select) for detection of group B Streptococcus from vaginal-rectal specimens.
We compared StrepB Select medium (Select) after enrichment with conventional culture for the detection of Group B Streptococcus (GBS). Postenrichment sensitivities of Select and conventional culture were 98.8% and 92.2%, respectively (P<0.05). Select was superior for detection of GBS from vaginal-rectal specimens. Growth of non-GBS colonies required additional work to exclude the presence of GBS, especially after 48 h of incubation. Incubation of Select beyond 24 h did not significantly increase the yield of GBS.
Department of Laboratory Medicine and Pathobiology, Sunnybrook Health Sciences Centre, University of Toronto, Rm. B1-03, 2075 Bayview Avenue, Toronto, Ontario, Canada M4N 3M5. lisa.louie@sunnybrook.ca
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Louie
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Kotowich
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Meaney
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Vearncombe
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Simor
D001431:Bacteriological Techniques / Q000379:methods*; D002863:Chromogenic Compounds / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry*; D005260:Female; D006801:Humans; D010502:Perineum / Q000382:microbiology; D012007:Rectum / Q000382:microbiology*; D012680:Sensitivity and Specificity; D013290:Streptococcal Infections / Q000175:diagnosis* / Q000382:microbiology; D013292:Streptococcus agalactiae / Q000302:isolation & purification*; D014621:Vagina / Q000382:microbiology*
D002863:Chromogenic Compounds; D003470:Culture Media