A proteomic investigation of B lymphocytes in an autistic family: a pilot study of exposure to natural rubber latex (NRL) may lead to autism.
Autism is a multi-factorial neurodevelopmental disorder. We have investigated the molecular mechanism involved in a Chinese family with autism by a proteomic approach. Antibody chips containing 500 spots of human protein antibodies were used to screen for differentially expressed proteins in the peripheral B lymphocytes between autistic and non-autistic siblings in this family. Four proteins relevant to immuno-pathway, including IKKα that was up-regulated and Tyk2, EIF4G1 and PRKCI that were down-regulated, were identified differentially expressed in autistic versus non-autistic siblings. Western blot analysis and reverse transcription quantitative polymerase chain reaction validated the differential expression of these four proteins. Based on the function of these differentially expressed proteins, relevant studies on immunoglobulin E (IgE) level, nuclear factor kappa B signaling activation and cell cycle were conducted in both autistic and non-autistic children of this family. Considering the fact that the family members were in close contact with natural rubber latex (NRL) and that IgE-mediated cross-reactions could be triggered by Hevea brasiliensis (Hev-b) proteins in NRL, we hypothesize that immune reactions triggered by close contact with NRL might influence the functions of B lymphocytes by altering expression of certain proteins identified in our experiments thus contributing to the occurrence of autism.
Peking University Center of Medical Genetics, Beijing, 100191, China.
Chen
C
Shen
Xin-liang
XL
Zhao
Weina
W
Ju
Xiao-bing
XB
Zou
Li-rong
LR
Huo
Wu
W
Yan
Jun-hua
JH
Zou
Guo-di
GD
Yan
Edmund C
EC
Jenkins
W Ted
WT
Brown
Nanbert
N
Zhong
D000818:Animals; D000906:Antibodies / Q000032:analysis / Q000276:immunology; D044466:Asian People; D001321:Autistic Disorder / Q000209:etiology* / Q000276:immunology*; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000502:physiology; D002453:Cell Cycle / Q000502:physiology; D002460:Cell Line; D002648:Child; D002675:Child, Preschool; D005260:Female; D028482:Hevea / Q000737:chemistry / Q000276:immunology; D006801:Humans; D007840:Latex / Q000276:immunology*; D020315:Latex Hypersensitivity / Q000276:immunology*; D008297:Male; D010375:Pedigree; D010865:Pilot Projects; D040081:Protein Array Analysis; D020543:Proteome / Q000032:analysis*; D012408:Rubber / Q000737:chemistry*
D000906:Antibodies; D007840:Latex; D020543:Proteome; D012408:Rubber