[Long-lived radicals of amino acids induced by X-ray-radiation are the source of hydrogen peroxide in aqueous medium].
The formation of long-lived radicals in the solutions of casein and its hydrolysate with an equimolar mixture of amino acids was compared by measuring the X-ray-induced chemiluminescence. It was shown that free amino acids constituting the protein produce long-lived radicals. It was demonstrated that some amino acids (Leu, Ile, Val, Ser, Trp, Met, Pro, Arg, Gly, Phe) emit light of visible spectrum over a long period of time after the irradiation, which indicates the generation of long-lived radicals of these amino acids. The half-life times of these radicals are several hours. Dissolving irradiated dry amino acids capable of luminescing over a long time gives rise to the formation of hydrogen peroxide in aqueous medium.
Gudkov
S V
SV
Garmash
S A
SA
Karp
O E
OE
Smirnova
V S
VS
Chernikov
A V
AV
Bruskov
V I
VI
D000596:Amino Acids / Q000737:chemistry / Q000528:radiation effects*; D002364:Caseins / Q000737:chemistry / Q000528:radiation effects; D005609:Free Radicals / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000737:chemistry*; D006868:Hydrolysis; D008163:Luminescent Measurements; D011487:Protein Conformation; D014867:Water / Q000737:chemistry*
D000596:Amino Acids; D002364:Caseins; D005609:Free Radicals; D014867:Water; D006861:Hydrogen Peroxide