Selection of genetically diverse Trichoderma spp. isolates for suppression of Phytophthora capsici on bell pepper.
Environmentally compatible control measures are needed for suppression of Phytophthora capsici on pepper. Twenty-three isolates of Trichoderma were screened for suppression of a mixture of 4 genetically distinct isolates of this pathogen on bell pepper (Capsicum anuum) in greenhouse pot assays. Of these 23 isolates, GL12, GL13, and Th23 provided significant suppression of P. capsici in at least 2 assays. These isolates were then compared with Trichoderma virens isolates GL3 and GL21 for suppression of this disease in the presence and absence of the harpin-based natural product Messenger. Isolates GL3 and Th23 provided significant disease suppression (P ≤ 0.05) in 3 of 4 assays, while GL12, GL13, and GL21 provided significant suppression in 2 of 4 assays. There was no apparent benefit from the application of Messenger. Phylogenetic analysis of these 5 isolates (based on the ITS1 region of the nuclear rDNA cluster and tef1), and an additional 9 isolates that suppressed P. capsici in at least 1 assay, separated isolates into 2 clades, with 1 clade containing GL3, GL12, GL13, and GL21. There were also 2 more distantly related isolates, one of which was Th23. We report here the identification of genetically distinct Trichoderma isolates for potential use in disease management strategies employing isolate combinations directed at suppression of P. capsici on pepper.
Roberts
Daniel P
DP
Henry A. Wallace Beltsville Agricultural Research Center, MD 20705-2350, USA. dan.roberts@ars.usda.gov
Maul
Jude E
JE
McKenna
Laurie F
LF
Emche
Sarah E
SE
Meyer
Susan L F
SL
Collins
Ronald T
RT
Bowers
John H
JH
D000898:Antibiosis; D002212:Capsicum / Q000469:parasitology*; D004271:DNA, Fungal / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D010572:Pest Control, Biological / Q000379:methods*; D010838:Phytophthora / Q000254:growth & development*; D010935:Plant Diseases / Q000469:parasitology*; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D012987:Soil / Q000469:parasitology; D014242:Trichoderma / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology*; D014675:Vegetables / Q000382:microbiology
D004271:DNA, Fungal; D004275:DNA, Ribosomal; D012987:Soil