Inhibition of CD44 N- and O-linked glycosylation decreases endometrial cell lines attachment to peritoneal mesothelial cells.
The attachment of endometrial epithelial cells (EECs) and endometrial stromal cells (ESCs) to peritoneal mesothelial cells (PMCs) with and without inhibition of N- and O-linked glycosylation, the viability of EECs and ESCs, and the expression of CD44 surface density were evaluated. Inhibition of CD44 N- and O-linked glycosylation by using tunicamycin and/or B-GalNAc statistically significantly inhibited endometrial cell attachment to peritoneal mesothelial cells, suggesting a role in establishment of early endometriotic lesions.
Department of Obstetrics and Gynecology, University of Texas Health Science Center at San Antonio, Texas 78229, USA.
Allison K
AK
Rodgers
Anitha
A
Nair
Peter A
PA
Binkley
Rajeshwar
R
Tekmal
Robert S
RS
Schenken
D000116:Acetylgalactosamine / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use; D001593:Benzyl Compounds / Q000494:pharmacology* / Q000627:therapeutic use; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004353:Drug Evaluation, Preclinical; D004715:Endometriosis / Q000473:pathology / Q000517:prevention & control; D004717:Endometrium / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D004791:Enzyme Inhibitors / Q000494:pharmacology / Q000627:therapeutic use; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D004848:Epithelium / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D005260:Female; D006031:Glycosylation; D006801:Humans; D018960:Hyaluronan Receptors / Q000378:metabolism*; D010529:Peritoneal Cavity / Q000166:cytology; D010532:Peritoneal Diseases / Q000473:pathology / Q000517:prevention & control; D011499:Protein Processing, Post-Translational / Q000187:drug effects / Q000502:physiology
D001593:Benzyl Compounds; C497483:CD44 protein, human; D004791:Enzyme Inhibitors; D018960:Hyaluronan Receptors; C080739:benzyl-alpha-N-acetylgalactosamine; D000116:Acetylgalactosamine