A mammalian insulysin homolog is regulated by enzyme IIA(Glc) of the glucose transport system in Vibrio vulnificus.
Vibrio vulnificus is an opportunistic human pathogen that causes severe infections in susceptible individuals. While the components of the Escherichia coli phosphoenolpyruvate: sugar phosphotransferase system (PTS) have been shown to regulate numerous targets, little such information is available for the V. vulnificus PTS. Here we show that enzyme IIA(Glc) of the PTS regulates the peptidase activity of a mammalian insulysin homolog in V. vulnificus. While interaction of IIA(Glc) with the insulysin homolog is independent of the phosphorylation state of IIA(Glc), only unphosphorylated IIA(Glc) activates the insulysin homolog. Taken together, our results suggest that the V. vulnificus insulysin-IIA(Glc) complex plays a role in survival in the host by sensing glucose.
Kim
You-Jin
YJ
Department of Biophysics and Chemical Biology and Institute of Microbiology, Seoul National University, Seoul 151-742, Republic of Korea.
Ryu
Yangkyun
Y
Koo
Byoung-Mo
BM
Lee
Na Yeon
NY
Chun
Se-Jin
SJ
Park
Soon-Jung
SJ
Lee
Kyu-Ho
KH
Seok
Yeong-Jae
YJ
D000818:Animals; D001692:Biological Transport; D005260:Female; D005947:Glucose / Q000378:metabolism*; D006801:Humans; D007339:Insulysin / Q000737:chemistry* / Q000378:metabolism*; D051379:Mice; D008813:Mice, Inbred ICR; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000378:metabolism*; D010766:Phosphorylation; D017386:Sequence Homology, Amino Acid; D016019:Survival Analysis; D041261:Vibrio vulnificus / Q000201:enzymology* / Q000378:metabolism / Q000472:pathogenicity
D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; D007339:Insulysin; D005947:Glucose