A novel and efficient model of coronary artery ligation and myocardial infarction in the mouse.
BACKGROUND
coronary artery ligation to induce myocardial infarction (MI) in mice is typically performed by an invasive and time-consuming approach that requires ventilation and chest opening (classic method), often resulting in extensive tissue damage and high mortality. We developed a novel and rapid surgical method to induce MI that does not require ventilation.


OBJECTIVE
the purpose of this study was to develop and comprehensively describe this method and directly compare it to the classic method.


RESULTS
male C57/B6 mice were grouped into 4 groups: new method MI (MI-N) or sham (S-N) and classic method MI (MI-C) or sham (S-C). In the new method, heart was manually exposed without intubation through a small incision and MI was induced. In the classic method, MI was induced through a ventilated thoracotomy. Similar groups were used in an ischemia/reperfusion injury model. This novel MI procedure is rapid, with an average procedure time of 1.22 ± 0.05 minutes, whereas the classic method requires 23.2 ± 0.6 minutes per procedure. Surgical mortality was 3% in MI-N and 15.9% in MI-C. The rate of arrhythmia was significantly lower in MI-N. The postsurgical levels of tumor necrosis factor-α and myeloperoxidase were lower in new method, indicating less inflammation. Overall, 28-day post-MI survival rate was 68% with MI-N and 48% with MI-C. Importantly, there was no difference in infarct size or post-MI cardiac function between the methods.


CONCLUSIONS
this new rapid method of MI in mice represents a more efficient and less damaging model of myocardial ischemic injury compared with the classic method.
Center for Translational Medicine, George Zallie and Family Laboratory for Cardiovascular Gene Therapy, Thomas Jefferson University, 1025 Walnut St, Room 302, Philadelphia, PA 19107, USA. erhe.gao@jefferson.edu
Erhe
E
Gao
Yong Hong
YH
Lei
Xiying
X
Shang
Z Maggie
ZM
Huang
Lin
L
Zuo
Matthieu
M
Boucher
Qian
Q
Fan
J Kurt
JK
Chuprun
Xin L
XL
Ma
Walter J
WJ
Koch
D000818:Animals; D003331:Coronary Vessels / Q000601:surgery*; D004195:Disease Models, Animal; D008026:Ligation / Q000009:adverse effects / Q000379:methods; D008297:Male; D008722:Methods; D051379:Mice; D008810:Mice, Inbred C57BL; D009203:Myocardial Infarction / Q000209:etiology*; D012107:Research Design / Q000592:standards*; D013997:Time Factors