Fatty acid pattern of pancreatic islet lipids in Goto-Kakizaki rats.
Perturbations of fatty acid content and pattern were recently documented in epididymal and parametrial lipids, as well as plasma, liver, spleen, and brain phospholipids and triglycerides of Goto-Kakizaki rats (GK). This study extends such an investigation to pancreatic islets from both control and GK rats. Groups of 5,500-14,560 islets were obtained from either control or GK rats (n = 3 in each case) and examined for their lipid fatty acid content. In the islet triglycerides, the major difference between control and GK rats, i.e., a higher C18:2ω6 content in GK rats, was similar to that found in liver triglycerides. In the islet phospholipids, however, a number of differences between control and GK rats, concerning saturated, monodesaturated, and long-chain polyunsaturated ω3 and ω6 fatty acids, were often not similar to those found in liver phospholipids. The present study reveals a number of anomalies in the fatty acid pattern of islet phospholipids in GK rats, often differing from those encountered in liver phospholipids. Such a tissue specificity was borne out by the finding that, even in control animals, the situation found in islet phospholipids differed from that recorded in liver phospholipids.
Giroix
Marie-Hélène
MH
Laboratory of Biology and Pathology of Endocrine Pancreas, Unit of Functional and Adaptative Biology, Université Paris-Diderot/CNRS, Paris, France.
Louchami
Karim
K
Carpentier
Yvon A
YA
Sener
Abdullah
A
Malaisse
Willy J
WJ
D000818:Animals; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism*; D007515:Islets of Langerhans / Q000378:metabolism*; D019787:Linoleic Acid / Q000378:metabolism; D050356:Lipid Metabolism; D008297:Male; D009928:Organ Specificity; D010743:Phospholipids / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D017208:Rats, Wistar; D014280:Triglycerides / Q000378:metabolism
D005227:Fatty Acids; D010743:Phospholipids; D014280:Triglycerides; D019787:Linoleic Acid