CXCL12 gene polymorphism and hematologic recovery after transplantation of peripheral blood progenitor cells.
Previous studies have documented that the (3'-UTR,801A>G) polymorphism of the gene encoding stromal cell-derived factor-1 (SDF-1/CXCL12) is associated with the release of CD34+ cells from the bone marrow to the peripheral circulation in autologous transplant recipients and healthy donors of allogeneic transplants. The aim of this study was to investigate whether the CXCL12 gene polymorphism is associated with the accommodation of hematopoietic stem cells in the marrow, reflected by the recovery of granulocytes and platelets after autologous transplantation of peripheral blood progenitor cells (PBPCT). CXCL12 genotyping was performed in 57 recipients of autologous PBPCs using a PCR-RFLP technique and MspI restriction enzyme. The pace of granulocyte recovery significantly correlated with the pace of platelet recovery (P<.001, Spearman's R test). Hematologic recovery was observed to be affected by the CXCL12 gene polymorphism. Patients carrying the CXCL12-3'A allele were characterized by faster recovery of both granulocytes (median, 15 vs 17 days after PBPCT; P=.027) and platelets (17 vs 21 days after PBPCT; P=.023; Mann-Whitney U test). Multiple regression analyses, considering patient age/gender, number of transplanted CD34 cells, and CXCL12-3'A allele, confirmed the independent association of the CXCL12-3'A allele with granulocyte and platelet recovery after transplantation (P=.020 in both cases).
L. Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland.
A
A
Gieryng
K
K
Bogunia-Kubik
A
A
Lange
D000328:Adult; D000483:Alleles; D001853:Bone Marrow / Q000473:pathology*; D054377:Chemokine CXCL12 / Q000235:genetics*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D036102:Peripheral Blood Stem Cell Transplantation; D011110:Polymorphism, Genetic; D016896:Treatment Outcome
C515255:CXCL12 protein, human; D054377:Chemokine CXCL12