Genome analysis of Bifidobacterium bifidum PRL2010 reveals metabolic pathways for host-derived glycan foraging.
The human intestine is densely populated by a microbial consortium whose metabolic activities are influenced by, among others, bifidobacteria. However, the genetic basis of adaptation of bifidobacteria to the human gut is poorly understood. Analysis of the 2,214,650-bp genome of Bifidobacterium bifidum PRL2010, a strain isolated from infant stool, revealed a nutrient-acquisition strategy that targets host-derived glycans, such as those present in mucin. Proteome and transcriptome profiling revealed a set of chromosomal loci responsible for mucin metabolism that appear to be under common transcriptional control and with predicted functions that allow degradation of various O-linked glycans in mucin. Conservation of the latter gene clusters in various B. bifidum strains supports the notion that host-derived glycan catabolism is an important colonization factor for B. bifidum with concomitant impact on intestinal microbiota ecology.
Turroni
Francesca
F
Laboratory of Probiogenomics, Department of Genetics, Biology of Microorganisms, Anthropology and Evolution, University of Parma, 43100 Parma, Italy.
Bottacini
Francesca
F
Foroni
Elena
E
Mulder
Imke
I
Kim
Jae-Han
JH
Zomer
Aldert
A
Sánchez
Borja
B
Bidossi
Alessandro
A
Ferrarini
Alberto
A
Giubellini
Vanessa
V
Delledonne
Massimo
M
Henrissat
Bernard
B
Coutinho
Pedro
P
Oggioni
Marco
M
Fitzgerald
Gerald F
GF
Mills
David
D
Margolles
Abelardo
A
Kelly
Denise
D
van Sinderen
Douwe
D
Ventura
Marco
M
D001644:Bifidobacterium / Q000235:genetics* / Q000254:growth & development; D005243:Feces / Q000382:microbiology; D020869:Gene Expression Profiling; D016680:Genome, Bacterial; D023281:Genomics / Q000379:methods; D054884:Host-Pathogen Interactions / Q000235:genetics*; D006801:Humans; D007231:Infant, Newborn; D007422:Intestines / Q000382:microbiology; D053858:Metabolic Networks and Pathways / Q000235:genetics*; D008969:Molecular Sequence Data; D009077:Mucins / Q000378:metabolism; D005810:Multigene Family; D011134:Polysaccharides / Q000378:metabolism*
D009077:Mucins; D011134:Polysaccharides