Kroppenstedtia eburnea gen. nov., sp. nov., a thermoactinomycete isolated by environmental screening, and emended description of the family Thermoactinomycetaceae Matsuo et al. 2006 emend. Yassin et al. 2009.
A Gram-positive, spore-forming, aerobic, filamentous bacterium, strain JFMB-ATE(T), was isolated in 2008 during environmental screening of a plastic surface in grade C in a contract manufacturing organization in southern Germany. The isolate grew at temperatures of 25-50 °C and at pH 5.0-8.5, forming ivory-coloured colonies with sparse white aerial mycelia. Chemotaxonomic and molecular characteristics of the isolate matched those described for members of the family Thermoactinomycetaceae, except that the cell-wall peptidoglycan contained LL-diaminopimelic acid, while all previously described members of this family display this diagnostic diamino acid in meso-conformation. The DNA G+C content of the novel strain was 54.6 mol%, the main polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol, and the major menaquinone was MK-7. The major fatty acids had saturated C₁₄-C₁₆ branched chains. No diagnostic sugars were detected. Based on the chemotaxonomic results and 16S rRNA gene sequence analysis, the isolate is proposed to represent a novel genus and species, Kroppenstedtia eburnea gen. nov. sp. nov. The type strain is JFMB-ATE(T) ( = DSM 45196(T)  = NRRL B-24804(T)  = CCUG 59226(T)).
DSMZ - German Collection of Microorganisms and Cell Cultures, 38124 Braunschweig, Germany.
Mathias
M
von Jan
Microbiology, Vetter Pharma-Fertigung GmbH & Co. KG, 88212 Ravensburg, Germany.
Nicole
N
Riegger
DSMZ - German Collection of Microorganisms and Cell Cultures, 38124 Braunschweig, Germany.
Gabriele
G
Pötter
DSMZ - German Collection of Microorganisms and Cell Cultures, 38124 Braunschweig, Germany.
Peter
P
Schumann
DSMZ - German Collection of Microorganisms and Cell Cultures, 38124 Braunschweig, Germany.
Susanne
S
Verbarg
DSMZ - German Collection of Microorganisms and Cell Cultures, 38124 Braunschweig, Germany.
Cathrin
C
Spröer
HZI - Helmholtz Centre for Infection Research, 38124 Braunschweig, Germany.
Manfred
M
Rohde
Microbiology, Vetter Pharma-Fertigung GmbH & Co. KG, 88212 Ravensburg, Germany.
Bettina
B
Lauer
National Center for Agricultural Utilization Research, USDA-ARS, Peoria, IL 61604, USA.
David P
DP
Labeda
DSMZ - German Collection of Microorganisms and Cell Cultures, 38124 Braunschweig, Germany.
Hans-Peter
HP
Klenk
D056449:Bacillales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002473:Cell Wall / Q000737:chemistry; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D003960:Diaminopimelic Acid / Q000032:analysis; D004783:Environmental Microbiology; D005858:Germany; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D010457:Peptidoglycan / Q000737:chemistry; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D010860:Pigments, Biological / Q000378:metabolism; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013171:Spores, Bacterial / Q000166:cytology; D013696:Temperature
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D010457:Peptidoglycan; D010743:Phospholipids; D010860:Pigments, Biological; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D003960:Diaminopimelic Acid