Preliminary immunohistochemical study of natriuretic peptide receptor localization in canine and feline heart.
We examined the immunohistochemical distributions of natriuretic peptide receptor (NPR)-A, -B and -C that bind with natriuretic peptide hormones A, B and C in four healthy crossbreed young canine and feline cardiac tissues using specific antibodies against human antigens. Cross-immunoreactivities between antigens and antibodies were confirmed using western blot analysis. NPR-A and -C were expressed more strongly in dogs than cats. In both species, these expressions were stronger in the atria than the ventricles, with stronger expression in the left ventricles than the right. NPR-B was largely very weekly or undetected. In canine and feline cardiac tissues, the expressional distribution of NPR-A, -B, and -C closely matched with that of atrial natriuretic peptide, brain natriuretic peptide, and C-type natriuretic peptide as the ligands for corresponding receptors.
Laboratory of Veterinary Anatomy, Nippon Veterinary and Life Science University, Tokyo.
Tetsuya
T
Yamane
Naoyuki
N
Takemura
Hajime
H
Inoue
Satoshi
S
Soeta
Motoharu
M
Oishi
Hajime
H
Amasaki
D000818:Animals; D002415:Cats / Q000378:metabolism*; D004285:Dogs / Q000378:metabolism*; D005786:Gene Expression Regulation; D007150:Immunohistochemistry / Q000662:veterinary*; D009206:Myocardium / Q000378:metabolism*; D045265:Natriuretic Peptides / Q000235:genetics / Q000378:metabolism; D017461:Receptors, Atrial Natriuretic Factor / Q000235:genetics / Q000378:metabolism*
D045265:Natriuretic Peptides; D017461:Receptors, Atrial Natriuretic Factor