The effect of CD34 count and clonogenic potential of hematopoietic stem cells on engraftment.
In this study we have determined that the number of the CD34 (+) cells in the grafts that were infused to 48 patients who underwent autologous and allogeneic hematopoietic cell transplantation and evaluated the number of colony forming units in vitro. Our aim was to determine whether there is a relation between these cell counts and post transplantation engraftment kinetics. A negative correlation was detected (p<0.05) between the CD34 (+) cell count and all colony forming units. A correlation between the CD34 (+) cell count and the kinetics of engraftment could not be demonstrated. In the autologous group, only a weak negative correlation between the CFU-GEMM and neutrophil engraftment was detected. In the allogeneic group, colony forming units did not determine the engraftment.
Department of Cardiovascular Surgery, Ankara Bayındır Hospital, Turkey.
Tamer
T
Sarı
Department of Hematology and Stem Cell Transplantation Unit, Ankara Oncology Research and Education Hospital, 06200 Demetevler Yenimahalle, Ankara, Turkey. Electronic address: meltemkurt@hotmail.com.
Meltem Kurt
MK
Yüksel
Department of Hematology, Ankara University School of Medicine, Turkey.
Pervin
P
Topçuoğlu
Hemapheresis Unit, Ankara University School of Medicine, Turkey.
Meltem
M
Tol
Hemapheresis Unit, Ankara University School of Medicine, Turkey.
Erol
E
Ayyıldız
Department of Hematology, Ankara University School of Medicine, Turkey.
Muhit
M
Özcan
Department of Hematology, Ankara University School of Medicine, Turkey.
Osman
O
İlhan
D000293:Adolescent; D000328:Adult; D000368:Aged; D018952:Antigens, CD34; D002452:Cell Count; D002999:Clone Cells; D003114:Colony-Forming Units Assay; D005260:Female; D006085:Graft Survival; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods* / Q000592:standards; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D014182:Transplantation, Autologous; D014184:Transplantation, Homologous; D055815:Young Adult
D018952:Antigens, CD34