Pseudonocardia kunmingensis sp. nov., an actinobacterium isolated from surface-sterilized roots of Artemisia annua L.
A Gram-positive, aerobic, actinobacterial strain with rod-shaped spores, designated YIM 63158(T), was isolated from the surface-sterilized roots of Artemisia annua L. collected from Yunnan province, south-west China. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YIM 63158(T) belonged to the genus Pseudonocardia. The closest neighbours were 'Pseudonocardia sichuanensis' KLBMP 1115 (99.9 % 16S rRNA gene sequence similarity), Pseudonocardia adelaidensis EUM 221(T) (99.1 %) and Pseudonocardia zijingensis DSM 44774(T) (98.8 %); sequence similarities to other members of the genus Pseudonocardia ranged from 98.6 to 94.4 %. The chemotaxonomic characteristics, such as the cell-wall diaminopimelic acid, whole-cell sugars, fatty acid components and major menaquinones, suggested that the isolate belonged to the genus Pseudonocardia. The G+C content of the genomic DNA was 73.3 mol%. On the basis of physiological, biochemical and chemotaxonomic data, including low DNA-DNA relatedness between the isolate and other members of the genus Pseudonocardia, it is proposed that strain YIM 63158(T) represents a novel species in this genus, with the name Pseudonocardia kunmingensis sp. nov. The type strain is YIM 63158(T) ( = DSM 45301(T)  = CCTCC AA 208078(T)). [corrected].
Zhao
Guo-Zhen
GZ
The Key Laboratory for Microbial Resources of the Ministry of Education, PR China and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, PR China.
Li
Jie
J
Key Laboratory of Marine Bio-resources Sustainable Utilization/Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, PR China.|The Key Laboratory for Microbial Resources of the Ministry of Education, PR China and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, PR China.
Huang
Hai-Yu
HY
The Key Laboratory for Microbial Resources of the Ministry of Education, PR China and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, PR China.
Zhu
Wen-Yong
WY
The Key Laboratory for Microbial Resources of the Ministry of Education, PR China and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, PR China.
Park
Dong-Jin
DJ
Korea Research Institutes of Biosciences and Biotechnology 52, Eoeun-dong, Yuseong gu, Daejeon, 305-333, Republic of Korea.
Kim
Chang-Jin
CJ
Korea Research Institutes of Biosciences and Biotechnology 52, Eoeun-dong, Yuseong gu, Daejeon, 305-333, Republic of Korea.
Xu
Li-Hua
LH
The Key Laboratory for Microbial Resources of the Ministry of Education, PR China and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, PR China.
Li
Wen-Jun
WJ
Key Laboratory of Marine Bio-resources Sustainable Utilization/Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, PR China.|The Key Laboratory for Microbial Resources of the Ministry of Education, PR China and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, PR China.
D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D000332:Aerobiosis; D031172:Artemisia annua / Q000382:microbiology*; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002241:Carbohydrates / Q000032:analysis; D002473:Cell Wall / Q000737:chemistry; D002681:China; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D003960:Diaminopimelic Acid / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology*; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
D002241:Carbohydrates; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D003960:Diaminopimelic Acid