Study of histopathological, morphological and immunohistochemical features of recurrent pleomorphic adenoma: an attempt to predict recurrence of pleomorphic adenoma.
BACKGROUND
Recurrent pleomorphic adenoma (RPA) is an uncommon and challenging disease. The aim of this study was to review the clinical information obtained by examining RPA patients, to determine its histomorphological features and to verify the expression of the Mcm-2 markers of cell proliferation and the expression of p-53 in pleomorphic adenoma (PA), RPA, and RPA with malignant transformation (TRPA).


METHODS
A total of 10 cases of PA and 29 cases of RPA were examined in detail for the presence of nodules and their histomorphological features. Cell proliferation and expression of p-53 were detected by the immunohistochemical technique using the antibodies against Mcm-2 and p-53.


RESULTS
Histopathologically, RPA is very similar to PA; the only difference found was that all the cases of RPA were multinodular. When comparing primary and recurrent tumor, no significant difference was found in terms of cell proliferation and the expression of p-53; however, in the RPA with areas of malignant transformation there was an increased expression of these proteins.


CONCLUSIONS
This study showed that recurrences were multinodular, with nodules varying in numbers and sizes. No significant difference in histological features was found between RPA and PA. Moreover, the immunohistochemical study showed a low expression of p-53 and Mcm-2 in PA and RPA and an increased expression of these proteins in the RPA with areas of malignant transformation.
São Leopoldo Mandic Institute and Research Center, Campinas, SP, Brazil. andresabs@hotmail.com
Andresa Borges
AB
Soares
Albina
A
Altemani
Vera Cavalcanti
VC
de Araújo
D008949:Adenoma, Pleomorphic / Q000378:metabolism* / Q000473:pathology; D000293:Adolescent; D000328:Adult; D018797:Cell Cycle Proteins / Q000096:biosynthesis; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic / Q000378:metabolism* / Q000473:pathology; D002648:Child; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D064111:Minichromosome Maintenance Complex Component 2; D009364:Neoplasm Recurrence, Local / Q000378:metabolism* / Q000473:pathology; D009687:Nuclear Proteins / Q000096:biosynthesis; D010307:Parotid Neoplasms / Q000378:metabolism* / Q000473:pathology; D018709:Statistics, Nonparametric; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis; D055815:Young Adult
D018797:Cell Cycle Proteins; D009687:Nuclear Proteins; D016159:Tumor Suppressor Protein p53; C084859:MCM2 protein, human; D064111:Minichromosome Maintenance Complex Component 2