A new method for detection of pandemic influenza virus using High Resolution Melting analysis of the neuraminidase gene.
Diagnostic methods based upon exclusive detection of haemagglutinin do not detect sequence variation in other gene segments of the Influenza A virus. A complementary approach is described based upon high-resolution melting curve analysis of the neuraminidase gene, an approach with the potential ability to detect small changes in the neuraminidase sequence without the need for specific probes.
National Influenza Centre, Valladolid, Spain. dvarillas@saludcastillayleon.es
David
D
Varillas
Jesus F
JF
Bermejo-Martin
Raquel
R
Almansa
Silvia
S
Rojo
Begoña
B
Nogueira
Jose María
JM
Eiros
Lucia
L
Rico
Verónica
V
Iglesias
Raul Ortiz
RO
de Lejarazu
D004587:Electrophoresis, Agar Gel / Q000379:methods; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics / Q000302:isolation & purification*; D007251:Influenza, Human / Q000175:diagnosis*; D009439:Neuraminidase / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D044366:Transition Temperature; D014764:Viral Proteins / Q000235:genetics*; D014773:Virology / Q000379:methods*
D014764:Viral Proteins; C487630:NA protein, influenza A virus; D009439:Neuraminidase