Pharmacokinetics of 5-aminofluorescein-albumin, a novel fluorescence marker of brain tumors during surgery.
In a phase I/II study, the new fluorescence marker 5-aminofluorescein covalently bound to human serum albumin (AFL-HSA) was intravenously administered to 10 patients with brain tumor 1 to 4 days before surgery, and AFL-HSA kinetics were determined to assess the optimum timing of dye administration. AFL-HSA was determined in serum and brain tissue by size exclusion chromatography with fluorescence detection. AFL-HSA disposition was quite similar to albumin kinetics with small volumes of distribution (volume of central compartment [V(c)] = 2.4 L; volume of distribution [Vd(β)] = 6.8 L; volume at steady state [V(ss)] = 5.9 L), a low clearance (mean Cl = 16.85 mL/h), and a long elimination half-life (mean T(1/2;β) = 307 hours). Predicted peak concentrations in the peripheral compartment occurred at 80.9 hours, the time point of optimal fluorescence intensity observed in brain tumors during surgery.
Department of Clinical Pharmacology and Pharmaco-epidemiology, University of Heidelberg, Heidelberg, Germany.
Reinhard
R
Ding
Eva
E
Frei
Mahmoudreza
M
Fardanesh
Hans-Hermann
HH
Schrenk
Paul
P
Kremer
Walter E
WE
Haefeli
D000368:Aged; D001932:Brain Neoplasms / Q000378:metabolism / Q000601:surgery*; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D005260:Female; D005452:Fluoresceins / Q000008:administration & dosage / Q000493:pharmacokinetics*; D005456:Fluorescent Dyes / Q000008:administration & dosage / Q000493:pharmacokinetics*; D005858:Germany; D006207:Half-Life; D006801:Humans; D007262:Infusions, Intravenous; D008297:Male; D008657:Metabolic Clearance Rate; D008875:Middle Aged; D008954:Models, Biological; D015336:Molecular Probe Techniques; D016343:Monitoring, Intraoperative / Q000379:methods*; D019635:Neurosurgical Procedures; D012709:Serum Albumin / Q000008:administration & dosage / Q000493:pharmacokinetics*; D013050:Spectrometry, Fluorescence; D014018:Tissue Distribution
D005452:Fluoresceins; D005456:Fluorescent Dyes; D012709:Serum Albumin; C030864:5-aminofluorescein