Methylated chrysin, a dimethoxy flavone, partially suppresses the development of liver preneoplastic lesions induced by N-nitrosodiethylamine in rats.
The modifying effect of chemically modified chrysin on formation of preneoplastic foci induced by N-nitrosodiethylamine (DEN) was investigated in male rats. Male Wistar rats were administered three intraperitoneal injections of DEN (200 mg/kg bodyweight) interspersed by 2 weeks with or without an oral dose of dimethoxy flavone (DMF 100 mg/kg bodyweight), 2 weeks after DEN initiation. The number of GST-Pi positive foci and proliferating cell nuclear antigen (PCNA)-positive cells were significantly suppressed by the administration of DMF. Real-time RT-PCR analysis revealed that DMF treatment increased mRNA expression levels of apoptotic proteins p53 and fas, cell cycle regulatory proteins chek 2, cdkn1a, rad 50, anti-inflammatory protein pparg whereas the mRNA expression levels of bcl-2 and prdx-2 were decreased compared to mRNA levels in DEN-treated group. Therefore, we propose that DMF partially suppresses the formation of preneoplastic lesions in rats following DEN exposure by regulating anti-inflammatory and apoptosis-promoting events and restoring the cellular redox balance altered by DEN.
Department of Biochemistry, University of Madras, Guindy Campus, Chennai, India.
Mahaboob S
MS
Khan
Devaraj
D
Halagowder
S Niranjali
SN
Devaraj
D000818:Animals; D001483:Base Sequence; D002273:Carcinogens / Q000633:toxicity*; D018797:Cell Cycle Proteins / Q000235:genetics; D017931:DNA Primers; D004260:DNA Repair / Q000235:genetics; D004052:Diethylnitrosamine / Q000633:toxicity*; D005419:Flavonoids / Q000494:pharmacology*; D005982:Glutathione Transferase / Q000378:metabolism; D007150:Immunohistochemistry; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D008745:Methylation; D011230:Precancerous Conditions / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction
D002273:Carcinogens; D018797:Cell Cycle Proteins; D017931:DNA Primers; D005419:Flavonoids; D018809:Proliferating Cell Nuclear Antigen; C043561:chrysin; D004052:Diethylnitrosamine; D005982:Glutathione Transferase