Efficient CNS gene delivery by intravenous injection.
We administered recombinant SV40-derived viral vectors (rSV40s) intravenously to mice with or without prior intraperitoneal injection of mannitol to deliver transgenes to the central nervous system (CNS). We detected transgene-expressing cells (mainly neurons) most prominently in the cortex and spinal cord; prior intraperitoneal mannitol injection increased CNS gene delivery tenfold. Intravenous injection of rSV40s, particularly with mannitol pretreatment, resulted in extensive expression of multiple transgenes throughout the CNS.
Louboutin
Jean-Pierre
JP
Department of Pathology, Thomas Jefferson University, Philadelphia, Pennsylvania, USA. jplouboutin@hotmail.com
Chekmasova
Alena A
AA
Marusich
Elena
E
Chowdhury
J Roy
JR
Strayer
David S
DS
D000818:Animals; D019556:COS Cells; D002540:Cerebral Cortex / Q000378:metabolism*; D002522:Chlorocebus aethiops; D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D007275:Injections, Intravenous; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D013539:Simian virus 40 / Q000235:genetics*; D013116:Spinal Cord / Q000378:metabolism*; D019076:Transgenes