MT1-MMP plays an important role in an invasive activity of malignant pleural mesothelioma cell.
Malignant pleural mesothelioma (MPM) has a poor prognosis and is a treatment resistant tumor, which is increasing in frequency throughout the world. The poor prognosis is due to the aggressive local invasiveness rather than distant metastasis. In this study, we established a cell line of malignant mesothelioma from a clinical specimen and assessed the relationship between the expression of MT1-MMP and the invasion ability of that line, as well as the cultured cells of several other lines, using the simple method that we created previously. We established a cell line from a clinical specimen from a patient with malignant mesothelioma. We assessed the invasive activities of MPM cells in an easy-to-prepare double-layered collagen gel hemisphere (DL-CGH) system that enabled us to visualize cell movements during invasion. To assess the role of MT1-MMP in the invasive activity of MPM cells, we knocked down its expression by RNA interference (RNAi). The invasion assay with DL-CGH revealed that a high expression of MT1-MMP in MPM cells was associated with aggressive invasive activity. The RNAi of MT1-MMP indicated that the expression of MT1-MMP might have a crucial role in the invasiveness of MPM cells. The MT1-MMP expression in MPM cells is related to their capacity for locally aggressive spreading into the pleura and the surrounding tissues, and MT1-MMP should be a suitable molecular target for the suppression of the invasiveness of MPM.
Division of Thoracic Surgery, Kobe University Graduate School of Medicine, Kobe, Japan.
Takefumi
T
Doi
Yoshimasa
Y
Maniwa
Yugo
Y
Tanaka
Shinya
S
Tane
Shotaro
S
Hashimoto
Yoshiharu
Y
Ohno
Wataru
W
Nishio
Yoshihiro
Y
Nishimura
Chiho
C
Ohbayashi
Yutaka
Y
Okita
Yoshitake
Y
Hayashi
Masahiro
M
Yoshimura
D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D055785:Gene Knockdown Techniques; D006801:Humans; D053511:Matrix Metalloproteinase 14 / Q000235:genetics / Q000502:physiology*; D008654:Mesothelioma / Q000235:genetics*; D009361:Neoplasm Invasiveness / Q000473:pathology; D010997:Pleural Neoplasms / Q000235:genetics*; D034622:RNA Interference
D053511:Matrix Metalloproteinase 14