Structures of 2-acetylaminofluorene modified DNA revisited: insight into conformational heterogeneity.
Despite the extensive data on dG-AAF, the major DNA adduct derived from the model carcinogen 2-acetylaminofluorene, little is known with respect to its solution structures. Here, we provide NMR/CD evidence for three conformers of dG-AAF in duplex DNA: major groove B-type (B), base-displaced stacked (S), and minor groove wedge (W). The S/B/W-conformational heterogeneities were found to be sensitive to the nature of the flanking DNA sequence contexts and pH.
Patnaik
Satyakam
S
Department of Biomedical and Pharmaceutical Sciences, College of Pharmacy, University of Rhode Island, Kingston, Rhode Island 02881, USA.
Cho
Bongsup P
BP
D015073:2-Acetylaminofluorene / Q000737:chemistry*; D002273:Carcinogens / Q000737:chemistry; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry*; D018736:DNA Adducts / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D008968:Molecular Conformation; D013696:Temperature
D002273:Carcinogens; D018736:DNA Adducts; D004247:DNA; D015073:2-Acetylaminofluorene