Multiple sites of vasopressin synthesis in the injured brain.
Previous studies have indicated that the primary targets for vasopressin actions on the injured brain are the cerebrovascular endothelium and astrocytes, and that vasopressin amplifies the posttraumatic production of proinflammatory mediators. Here, the controlled cortical impact model of traumatic brain injury in rats was used to identify the sources of vasopressin in the injured brain. Injury increased vasopressin synthesis in the hypothalamus and cerebral cortex adjacent to the posttraumatic lesion. In the cortex, vasopressin was predominantly produced by activated microglia/macrophages, and, to a lesser extent, by the cerebrovascular endothelium. These data further support the pathophysiological role of vasopressin in brain injury.
Department of Emergency Medicine, Alpert Medical School of Brown University, Providence, Rhode Island 02903, USA. Joanna_Szmydynger-Chodobska@brown.edu
Joanna
J
Szmydynger-Chodobska
Brian J
BJ
Zink
Adam
A
Chodobski
D000818:Animals; D001127:Arginine Vasopressin / Q000096:biosynthesis; D001923:Brain Chemistry / Q000502:physiology*; D001930:Brain Injuries / Q000378:metabolism* / Q000473:pathology; D002540:Cerebral Cortex / Q000293:injuries / Q000378:metabolism / Q000473:pathology; D004730:Endothelium, Vascular / Q000502:physiology; D006955:Hypernatremia / Q000378:metabolism; D007031:Hypothalamus / Q000293:injuries / Q000378:metabolism / Q000473:pathology; D007150:Immunohistochemistry; D008264:Macrophages / Q000502:physiology; D008297:Male; D017628:Microglia / Q000502:physiology; D018613:Microscopy, Confocal; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D020318:Rats, Long-Evans; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014667:Vasopressins / Q000096:biosynthesis*
D012333:RNA, Messenger; D014667:Vasopressins; D001127:Arginine Vasopressin