Logic-embedded vectors for intracellular partitioning, endosomal escape, and exocytosis of nanoparticles.
A new generation of nanocarriers, logic-embedded vectors (LEVs), is endowed with the ability to localize components at multiple intracellular sites, thus creating an opportunity for synergistic control of redundant or dual-hit pathways. LEV encoding elements include size, shape, charge, and surface chemistry. In this study, LEVs consist of porous silicon nanocarriers, programmed for cellular uptake and trafficking along the endosomal pathway, and surface-tailored iron oxide nanoparticles, programmed for endosomal sorting and partitioning of particles into unique cellular locations. In the presence of persistent endosomal localization of silicon nanocarriers, amine-functionalized nanoparticles are sorted into multiple vesicular bodies that form novel membrane-bound compartments compatible with cellular secretion, while chitosan-coated nanoparticles escape from endosomes and enter the cytosol. Encapsulation within the porous silicon matrix protects these nanoparticle surface-tailored properties, and enhances endosomal escape of chitosan-coated nanoparticles. Thus, LEVs provide a mechanism for shielded transport of nanoparticles to the lesion, cellular manipulation at multiple levels, and a means for targeting both within and between cells.
Serda
Rita E
RE
Department of NanoMedicine and Biomedical Engineering, University of Texas Health Science Center, 1825 Pressler Street, Suite 537, Houston, TX 77030, USA. rita.serda@uth.tmc.edu
Mack
Aaron
A
van de Ven
Anne L
AL
Ferrati
Silvia
S
Dunner
Kenneth
K
Godin
Biana
B
Chiappini
Ciro
C
Landry
Matthew
M
Brousseau
Louis
L
Liu
Xuewu
X
Bean
Andrew J
AJ
Ferrari
Mauro
M
D000818:Animals; D001692:Biological Transport; D002460:Cell Line; D004337:Drug Carriers / Q000737:chemistry / Q000378:metabolism*; D011992:Endosomes / Q000378:metabolism*; D005089:Exocytosis / Q000502:physiology; D008264:Macrophages / Q000378:metabolism; D051379:Mice; D053758:Nanoparticles
D004337:Drug Carriers