Bioassay-guided evaluation of antinociceptive effect of N-salicyloyltryptamine: a behavioral and electrophysiological approach.
We investigated the antinociceptive and nerve excitability effects of the N-salicyloyltryptamine (NST) NST-treated mice exhibited a significant decrease in the number of writhes when 100 and 200 mg/kg (i.p.) were administered (i.p.). This effect was not antagonized by naloxone (1.5 mg/kg, i.p.). NST inhibited the licking response of the injected paw when 100 and 200 mg/kg were administered (i.p.) to mice in the first and second phases of the formalin test. Because the antinociceptive effects could be associated with neuronal excitability inhibition, we performed the single sucrose gap technique and showed that NST (3.57 mM) significantly reduced (29.2%) amplitude of the compound action potential (CAP) suggesting a sodium channel effect induced by NST. Our results demonstrated an antinociceptive activity of the NST that could be, at least in part, associated to the reduction of the action potential amplitude. NST might represent an important tool for pain management.
Quintans
Lucindo J
LJ
Physiology Department, Federal University of Sergipe, "Prof. Aloísio de Campos" University Campus, Av. Marechal Rondom, s/n, CEP 49100-000 São Cristovão, SE, Brazil. lucindo_jr@yahoo.com.br
Silva
Davi A
DA
Siqueira
Jullyana S
JS
Araújo
Adriano A S
AA
Barreto
Rosana S S
RS
Bonjardim
Leonardo R
LR
Desantana
Josimari M
JM
De Lucca
Waldeci
W
Souza
Maria F V
MF
Gutierrez
Stanley J C
SJ
Barbosa-Filho
José Maria
JM
Santana-Filho
Valter J
VJ
Araújo
Demétrius A M
DA
Almeida
Reinaldo N
RN
D019342:Acetic Acid; D000200:Action Potentials / Q000187:drug effects; D000700:Analgesics / Q000494:pharmacology* / Q000627:therapeutic use; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects*; D001681:Biological Assay / Q000379:methods*; D003975:Diazepam / Q000627:therapeutic use; D055724:Electrophysiological Phenomena / Q000187:drug effects*; D005557:Formaldehyde; D007213:Indomethacin / Q000494:pharmacology; D008297:Male; D051379:Mice; D010146:Pain / Q000188:drug therapy; D045442:Rotarod Performance Test; D012459:Salicylates / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D013997:Time Factors; D014363:Tryptamines / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use
D000700:Analgesics; C492080:N-salicyloyltryptamine; D012459:Salicylates; D014363:Tryptamines; D005557:Formaldehyde; D003975:Diazepam; D019342:Acetic Acid; D007213:Indomethacin