Functionalized gold nanoparticles: a detailed in vivo multimodal microscopic brain distribution study.
In the present study, the in vivo distribution of polyelectrolyte multilayer coated gold nanoparticles is shown, starting from the living animal down to cellular level. The coating was designed with functional moieties to serve as a potential nano drug for prion disease. With near infrared time-domain imaging we followed the biodistribution in mice up to 7 days after intravenous injection of the nanoparticles. The peak concentration in the head of mice was detected between 19 and 24 h. The precise particle distribution in the brain was studied ex vivo by X-ray microtomography, confocal laser and fluorescence microscopy. We found that the particles mainly accumulate in the hippocampus, thalamus, hypothalamus, and the cerebral cortex.
NanoBioMed Lab @ LANADA (Laboratory for Nanodiagnostics, Drug Delivery and Analysis), CBM-Cluster in Biomedicine S.c.r.l., Basovizza, AREA Science Park, Trieste, Italy.
Fernanda
F
Sousa
Subhra
S
Mandal
Chiara
C
Garrovo
Alberto
A
Astolfo
Alois
A
Bonifacio
Diane
D
Latawiec
Ralf Hendrik
RH
Menk
Fulvia
F
Arfelli
Sabine
S
Huewel
Giuseppe
G
Legname
Hans-Joachim
HJ
Galla
Silke
S
Krol
D000818:Animals; D001921:Brain / Q000378:metabolism*; D004573:Electrolytes / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D006801:Humans; D007275:Injections, Intravenous; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000633:toxicity; D051379:Mice; D008856:Microscopy, Fluorescence; D012709:Serum Albumin / Q000737:chemistry; D014018:Tissue Distribution; D055114:X-Ray Microtomography
D004573:Electrolytes; D012709:Serum Albumin; D006046:Gold