Dual RMCE for efficient re-engineering of mouse mutant alleles.
We have developed dual recombinase-mediated cassette exchange (dRMCE) to efficiently re-engineer the thousands of available conditional alleles in mouse embryonic stem cells. dRMCE takes advantage of the wild-type loxP and FRT sites present in these conditional alleles and in many gene-trap lines. dRMCE is a scalable, flexible tool to introduce tags, reporters and mutant coding regions into an endogenous locus of interest in an easy and highly efficient manner.
Developmental Genetics, Department of Biomedicine, University of Basel, Basel, Switzerland.
Marco
M
Osterwalder
Antonella
A
Galli
Barry
B
Rosen
William C
WC
Skarnes
Rolf
R
Zeller
Javier
J
Lopez-Rios
D000483:Alleles; D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics; D002352:Carrier Proteins / Q000502:physiology; D053595:Embryonic Stem Cells / Q000378:metabolism*; D005818:Genetic Engineering / Q000379:methods*; D047908:Intracellular Signaling Peptides and Proteins; D051379:Mice; D009419:Nerve Tissue Proteins / Q000502:physiology; D009687:Nuclear Proteins / Q000502:physiology; D011401:Promoter Regions, Genetic; D045522:Recombinases / Q000502:physiology*; D011995:Recombination, Genetic; D051901:Smad4 Protein / Q000235:genetics
D051792:Basic Helix-Loop-Helix Proteins; D002352:Carrier Proteins; D047908:Intracellular Signaling Peptides and Proteins; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; D045522:Recombinases; D051901:Smad4 Protein; C498766:Hand2 protein, mouse; C490810:Smad4 protein, mouse; C483194:Zfp503 protein, mouse