Na(+)- and H(+)-dependent motility in the coral pathogen Vibrio shilonii.
In this work, we analyzed motility and the flagellar systems of the marine bacterium Vibrio shilonii. We show that this bacterium produces lateral flagella when seeded on soft agar plates at concentrations of 0.5% or 0.6%. However, at agar concentrations of 0.7%, cells become round and lose their flagella. The sodium channel blocker amiloride inhibits swimming of V. shilonii with the sheathed polar flagellum, but not swarming with lateral flagella. We also isolated and characterized the filament–hook–basal body of the polar flagellum. The proteins in this structure were analyzed by MS. Eight internal sequences matched with known flagellar proteins. The comparison of these sequences with the protein database from the complete genome of V. shilonii allows us to conclude that some components of the polar flagellum are encoded in two different clusters of flagellar genes, suggesting that this bacterium has a complex flagellar system, more complex possibly than other Vibrio species reported so far.
Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, México DF, Mexico.
Yael
Y
González
Daniela
D
Venegas
Guillermo
G
Mendoza-Hernandez
Laura
L
Camarena
Georges
G
Dreyfus
D000362:Agar; D000584:Amiloride / Q000494:pharmacology; D000595:Amino Acid Sequence; D000818:Animals; D037421:Anthozoa / Q000382:microbiology; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D005407:Flagella / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D005408:Flagellin / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D008124:Locomotion; D009068:Movement; D011522:Protons; D020539:Sequence Analysis, Protein; D012964:Sodium / Q000378:metabolism*; D053719:Tandem Mass Spectrometry; D014733:Vibrio / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure*; D014783:Viscosity
D001426:Bacterial Proteins; D011522:Protons; D005408:Flagellin; D000584:Amiloride; D000362:Agar; D012964:Sodium