Genome-wide approaches to monitor pre-mRNA splicing.
Pre-mRNA processing is an essential control-point in the gene expression pathway of eukaryotic organisms. The budding yeast Saccharomyces cerevisiae offers a powerful opportunity to examine the regulation of this pathway. In this chapter, we will describe methods that have been developed in our lab and others to examine pre-mRNA splicing from a genome-wide perspective in yeast. Our goal is to provide all of the necessary information--from microarray design to experimental setup to data analysis--to facilitate the widespread use of this technology.
Inada
Maki
M
Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York, USA.
Pleiss
Jeffrey A
JA
D018076:DNA, Complementary / Q000235:genetics; D016681:Genome, Fungal / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D012322:RNA Precursors / Q000235:genetics*; D012326:RNA Splicing / Q000235:genetics*; D012331:RNA, Fungal / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics
D018076:DNA, Complementary; D012322:RNA Precursors; D012331:RNA, Fungal