Fibrin/hyaluronic acid composite hydrogels as appropriate scaffolds for in vivo artificial cartilage implantation.
Hydrogels prepared from a mixture of fibrin and high-molecular weight (MW) hyaluronic acid (HA) were found to be suitable scaffolds for chondrocyte seeding and pig knee cartilage regeneration. Collagen in the hydrogels is not necessary for the formation of biomechanically stable tissue. Regenerated cartilage showed very good biomechanical and histological properties only 6 months after implantation. Notably, the quality of the healing process was dependent on the initial chondrocyte concentration of the scaffolds. These experiments were performed according to good laboratory practice (GLP).
Laboratory of Tissue Engineering, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, v.v.i., Prague, Czech Republic.
Michala
M
Rampichová
Eva
E
Filová
Ferdinand
F
Varga
Andriy
A
Lytvynets
Eva
E
Prosecká
Lucie
L
Koláčná
Jan
J
Motlík
Alois
A
Nečas
Luděk
L
Vajner
Jiří
J
Uhlík
Evžen
E
Amler
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D001696:Biomechanical Phenomena; D002356:Cartilage / Q000502:physiology / Q000601:surgery*; D019902:Chondrocytes / Q000166:cytology / Q000502:physiology; D020219:Chondrogenesis; D005337:Fibrin / Q000737:chemistry*; D006820:Hyaluronic Acid / Q000737:chemistry*; D020100:Hydrogels; D008297:Male; D008422:Materials Testing; D019736:Prostheses and Implants; D012038:Regeneration; D013552:Swine; D013556:Swine, Miniature; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
D001672:Biocompatible Materials; D020100:Hydrogels; D005337:Fibrin; D006820:Hyaluronic Acid