Measurement of B-type natriuretic peptide by two assays utilizing antibodies with different epitope specificity.
OBJECTIVE
To compare plasma BNP values determined by a conventional-type and a novel-type of BNP assays.


METHODS
Plasma samples (n=94) from HF patients were analyzed by the novel-type "Single Epitope Sandwich" (SES assay prototype) and the conventional-type Siemens ADVIA Centaur BNP assays. Both assays were calibrated using recombinant proBNP (expressed in E. coli).


RESULTS
The SES assay measured 1.2- to 7.2-fold (2.1±0.9; mean, SD) greater BNP concentrations compared to the Siemens assay. A subset of six samples (6.4%) demonstrated the largest (3- to 7.2-fold higher) difference.


CONCLUSIONS
The SES prototype assay appears to more accurately measure the absolute concentrations of BNP immunoreactive forms.
Tamm
Natalia N
NN
HyTest LTD, Intelligate, 6th floor, Joukahaisenkatu 6, 20520 Turku, Finland.
Semenov
Alexander G
AG
Seferian
Karina R
KR
Bereznikova
Anastasia V
AV
Murakami
Maryann M
MM
Apple
Fred S
FS
Koshkina
Ekaterina V
EV
Krasnoselsky
Mihail I
MI
Katrukha
Alexey G
AG
D000906:Antibodies; D000939:Epitopes / Q000276:immunology; D004926:Escherichia coli; D006801:Humans; D020097:Natriuretic Peptide, Brain / Q000097:blood; D010446:Peptide Fragments / Q000097:blood; D012680:Sensitivity and Specificity
D000906:Antibodies; D000939:Epitopes; D010446:Peptide Fragments; D020097:Natriuretic Peptide, Brain