Enzymatic amplification of DNA by PCR: standard procedures and optimization.
The polymerase chain reaction (PCR) is used to enzymatically amplify small quantities of specific DNA sequences. The reaction must optimized to specifically amplify the sequences and primers of interest; this includes titration of magnesium chloride and selection of enhancing agents, if appropriate, to minimize nonspecific primer target interactions and maximize the specificity, sensitivity, and yield.
Kramer
M F
MF
Harvard Medical School, Boston, Massachusetts, USA.
Coen
D M
DM
D004247:DNA / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000379:methods*; D014116:Toxicology / Q000379:methods
D004247:DNA