Measurement of ALA synthase activity.
In most cells, δ-aminolevulinate (ALA) synthase is the rate-limiting enzyme in heme synthesis. It is inducible by drugs and toxins and is feedback regulated by heme. This unit describes a radiometric assay using [¹⁴C]succinate as a substrate and a colorimetric assay based on the conversion of ALA to a pyrrole.
VA Medical Center, White River Junction, Vermont, USA.
P R
PR
Sinclair
N
N
Gorman
N W
NW
Cornell
D000624:5-Aminolevulinate Synthetase / Q000032:analysis*; D000622:Aminolevulinic Acid / Q000032:analysis; D000818:Animals; D002250:Carbon Radioisotopes; D003124:Colorimetry / Q000379:methods; D006801:Humans; D011758:Pyrroles / Q000032:analysis; D011874:Radiometry / Q000379:methods; D013386:Succinates
D002250:Carbon Radioisotopes; D011758:Pyrroles; D013386:Succinates; D000622:Aminolevulinic Acid; D000624:5-Aminolevulinate Synthetase