Isolation of photosystem II reaction center complexes from plants.
Methods to isolate and purify 6- and 5-Chl D1/D2/Cyt b559 photosystem II (PSII) reaction center (RC) complexes from plants are presented, and the advantages and disadvantages of each procedure are discussed. One of the simpler 6-Chl procedures and a procedure for isolating 5-Chl complexes are described in detail. Furthermore, a rapid procedure that produces relatively large amounts of less pure 6-Chl material (i.e., more nonpigmented protein) is also described. Criteria to assess the purity of PSII RC preparations are presented, and problems associated with each of the isolation procedures are discussed.
National Renewable Energy Laboratory, Basic Sciences Center, Golden, CO, USA.
Michael
M
Seibert
Rafael
R
Picorel
D002462:Cell Membrane / Q000201:enzymology; D005591:Chemical Fractionation / Q000379:methods*; D002734:Chlorophyll / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D017830:Octoxynol / Q000737:chemistry; D010446:Peptide Fragments / Q000032:analysis; D045332:Photosystem II Protein Complex / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D059828:Plant Cells; D010944:Plants / Q000201:enzymology*; D013056:Spectrophotometry, Ultraviolet; D013997:Time Factors
D010446:Peptide Fragments; D045332:Photosystem II Protein Complex; D002734:Chlorophyll; D017830:Octoxynol