In vivo cell tracking of canine allogenic mesenchymal stem cells administration via renal arterial catheterization and physiopathological effects on the kidney in two healthy dogs.
Stem cell therapy is being special premise for various renal diseases. However, there is limited literature on localization and pathologic and functional effects of allogenic mesenchymal stem cells (MSCs) in healthy dogs. Two healthy dogs were included in this study. Canine MSCs (cMSCs) were cultured from canine bone marrow and incubated with superparamagnetic iron oxide (SPIO) for in vivo cell tracking via MR imaging. The dogs were given the MSC (3 × 10(6) cells) into a renal artery via femoral artery catheterization. Follow-up serial renal assessments included ultrasonography and MRI, serum chemistry, urine analysis, and renal clearance tests. The dogs were euthanized at days 8 and 35 respectively for histopathologic evaluation of kidney. Strong hypointensity in MRI was detected in the treated renal cortex the day after cMSCs infusion. However they disappeared from MR image by the 8th day. Of the serum chemistry tests, serum hepatic enzymes (ALT, AST) were significantly elevated for one week after cMSCs treatment. Histopathological findings also revealed infiltration of SPIO-containing cells into the parenchyma of kidney. On 35th day, histopathology, glomerular atrophy, tubular necrosis, and mineralization were found in the subcapsular cortex, with fibrosis of the interstitial tissues. In vivo MRI studies of stem cells were useful in determining the sequential location of stem cells in the renal parenchyma of healthy dogs. Allogenic stem cells administered via renal artery caused inflammation, tubular necrosis, mineralization, and fibrosis without functional complications.
Department of Veterinary Internal Medicine, College of Veterinary Medicine, Konkuk University, Seoul, Korea.
Jong-Hyun
JH
Yoo
Chul
C
Park
Dong-In
DI
Jung
Chae-Young
CY
Lim
Byeong-Teck
BT
Kang
Jung-Hyun
JH
Kim
Jung-Won
JW
Park
Jae-Hoon
JH
Kim
Hee-Myung
HM
Park
D000410:Alanine Transaminase / Q000097:blood; D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001663:Bilirubin / Q000097:blood; D001806:Blood Urea Nitrogen; D058948:Cell Tracking / Q000379:methods / Q000662:veterinary*; D003404:Creatinine / Q000097:blood; D003911:Dextrans; D004285:Dogs / Q000502:physiology*; D005919:Glomerular Filtration Rate / Q000502:physiology / Q000662:veterinary; D007668:Kidney / Q000098:blood supply / Q000000981:diagnostic imaging / Q000503:physiopathology*; D008279:Magnetic Resonance Imaging / Q000379:methods / Q000662:veterinary; D058185:Magnetite Nanoparticles; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods / Q000662:veterinary*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000000981:diagnostic imaging / Q000502:physiology; D046529:Microscopy, Electron, Transmission / Q000662:veterinary; D014463:Ultrasonography; D005723:gamma-Glutamyltransferase / Q000097:blood
D003911:Dextrans; D058185:Magnetite Nanoparticles; D003404:Creatinine; D005723:gamma-Glutamyltransferase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000469:Alkaline Phosphatase; C065507:ferumoxides; D001663:Bilirubin