Contamination of commercial PCR master mix with DNA from Coxiella burnetii.
Contamination of an in-house diagnostic real-time PCR for Q fever was traced back to a commercially obtained PCR Master Mix. It was established that this Master Mix contained DNA from Coxiella burnetii, probably as a result of the use of compounds of animal origin such as bovine serum albumin.
Department of Medical Microbiology and Infectious Diseases, Canisius Wilhelmina Hospital, and Radboud University Nijmegen Medical Center, Weg door Jonkerbos 100, 6532 SZ Nijmegen, Netherlands.
Jeroen J H C
JJ
Tilburg
Marrigje H
MH
Nabuurs-Franssen
Erik J
EJ
van Hannen
Alphons M
AM
Horrevorts
Willem J G
WJ
Melchers
Corné H W
CH
Klaassen
D002021:Buffers; D016997:Coxiella burnetii / Q000235:genetics*; D004247:DNA / Q000032:analysis* / Q000235:genetics; D006801:Humans; D016133:Polymerase Chain Reaction; D011933:Reagent Kits, Diagnostic
D002021:Buffers; D011933:Reagent Kits, Diagnostic; D004247:DNA