Methylene blue-conjugated hydrogel nanoparticles and tumor-cell targeted photodynamic therapy.
Methylene blue-conjugated polyacrylamide nanoparticles are prepared through a microemulsion polymerization, after conjugation of the dye with a monomer. The nanoparticles have a 50-60 nm diameter in solution. This conjugation method enables a large increase in loading of methylene blue per nanoparticle and also minimizes dye leaching out of the nanoparticle. Furthermore, the dye content can be controlled by variation of the dye amount, enabling a more refined control of the singlet oxygen production ability. The nanoparticles are coated with F3 peptides, which give specific targeting to selected tumor cells, 9L, MDA-MB-435, and F98, in vitro. In addition, MTT assays reveal that the nanoparticles have no dark toxicity but excellent PDT efficacy increasing with the nanoparticle dose and irradiation time.
Department of Chemistry, University of Michigan, 930 N. University, Ann Arbor, Michigan 48109, USA.
Hoe Jin
HJ
Hah
Gwangseong
G
Kim
Yong-Eun Koo
YE
Lee
Daniel A
DA
Orringer
Oren
O
Sagher
Martin A
MA
Philbert
Raoul
R
Kopelman
D000180:Acrylic Resins / Q000737:chemistry; D000818:Animals; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004337:Drug Carriers; D016503:Drug Delivery Systems; D006801:Humans; D020100:Hydrogels; D008751:Methylene Blue / Q000737:chemistry* / Q000494:pharmacology; D053758:Nanoparticles; D009842:Oligopeptides / Q000737:chemistry; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000737:chemistry* / Q000494:pharmacology; D051381:Rats; D026082:Singlet Oxygen / Q000032:analysis; D000099166:Polyacrylamides
D000180:Acrylic Resins; D004337:Drug Carriers; D020100:Hydrogels; D008751:Methylene Blue; D009842:Oligopeptides; D017319:Photosensitizing Agents; D026082:Singlet Oxygen; D000099166:Polyacrylamides