Trichosanthin affects HSV-1 replication in Hep-2 cells.
Trichosanthin (TCS) is a type I ribosome-inactivating protein that inhibits the replication of both human immunodeficiency virus type 1 (HIV-1) and herpes simplex virus type 1 (HSV-1). The mechanism of inhibition is not clear. This investigation explored the effects of TCS on the stages of HSV-1 infection in Hep-2 cells, from attachment to release. We demonstrated that TCS reduced HSV-1 antigen and DNA content and interfered with viral replication as early as 3-15 h after infection. TCS had no effect on HSV-1 attachment, penetration or immediate-early gene expression. However, the expression of early and late genes and virion release were diminished. In summary, this study demonstrates that TCS primarily affects HSV-1 replication in Hep-2 cells during the early to late infection period.
School of Biomedical Sciences, The Chinese University of Hong Kong, Hong Kong SAR, China.
Dong-Xu
DX
He
Siu-Cheung
SC
Tam
D002460:Cell Line; D015870:Gene Expression / Q000187:drug effects; D018259:Herpesvirus 1, Human / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D006801:Humans; D015978:Trichosanthin / Q000494:pharmacology*; D057074:Virus Release / Q000187:drug effects*; D014779:Virus Replication / Q000187:drug effects*
D015978:Trichosanthin