Occurrence and distribution of various genetic structures of class 1 and class 2 integrons in Salmonella enterica isolates from foodborne disease patients in Korea for 16 years.
We have investigated the distribution of integrons among 752 multidrug-resistant Salmonella isolates from human febrile and/or diarrheal patients during 1992-2007 and analyzed their genetic characteristics. Here, we report extensive integron analysis results within human isolates during the last 16 years. The gene or gene cassette(s) in the class 1 integrons found in the isolates were dfrA7, dfrA12-orfF-aadA2, aadA2, bla(PSE1), dfrA1-aadA1, dfrA17-aadA5, bla(OXA1)-aadA1, aadB-aadA1, aadA22, aadA1, and aac6'Ib-bla(OXA1)-aadA2. Class 2 integrons harboring dfrA1-sat2-aadA1 gene cassette were also found in four isolates. Twenty-nine isolates including one Salmonella Schleissheim isolate had two integrons harboring aadA2 and bla(PSE1) in their variable regions of 1.0 and 1.2 kb amplicons, respectively, which have been also found in Salmonella genomic island 1 (SGI1) of multidrug-resistant Salmonella Typhimurium DT104. The presence of SGI1 in Salmonella Schleissheim isolate was proved by SGI1-specific polymerase chain reaction. We first report a Salmonella Schleissheim having SGI1, Salmonella Typhimurium and Salmonella Heidelberg having the class 2 integron with dfrA1-sat2-aadA1 cassettes, Salmonella London with the aac6'Ib-bla(OXA1)-aadA2 gene cassette, Salmonella Chailey with the gene cassette of aadA22, and coexistence of two class 1 integrons carrying aadA22 and dfrA12-orfF-aadA2 in Salmonella Typhimurium.
Kim
Shukho
S
Division of Enteric Bacterial Infections, Center for Infectious Diseases, Korea National Institute of Health, Seoul, Korea.
Kim
Sung-Hun
SH
Kim
Jungmin
J
Shin
Ji-Hyun
JH
Lee
Bok-Kwon
BK
Park
Mi-Sun
MS
D001483:Base Sequence; D017124:Conserved Sequence; D004251:DNA Transposable Elements; D030561:Databases, Nucleic Acid; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D005798:Genes, Bacterial; D019450:Genes, MDR; D044404:Genomic Islands; D006801:Humans; D032023:Integrons; D017720:Molecular Epidemiology; D016133:Polymerase Chain Reaction; D056910:Republic of Korea; D012478:Salmonella Food Poisoning / Q000382:microbiology*; D019779:Salmonella enterica / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D012703:Serotyping; D013045:Species Specificity
D004251:DNA Transposable Elements