Mode of action of plantaricin MG, a bacteriocin active against Salmonella typhimurium.
Plantaricin MG is a 2,180-Da bacteriocin produced by Lactobacillus plantarum KLDS1.0391, which was isolated from Chinese traditional fermented cream. Plantaricin MG showed a broad inhibitory activity against not only Gram-positive bacteria but also Gram-negative bacteria including Listeria monocytogenes and Salmonella typhimurium. The mode of action of plantaricin MG on S. typhimurium was reported in this article. The addition of plantaricin MG to energized cells of S. typhimurium dissipated both, the transmembrane potential (Δψ) and the pH gradient (ΔpH). Energized membrane, obtained after the addition of glucose, was more susceptible to plantaricin MG action, leading to the release of intracellular K(+)ions, inorganic phosphate, ATP and UV-absorbing materials. These data suggest that the presence of a proton motive force promotes the interaction of plantaricin MG with the cytoplasmic membrane of energized cells, leading to pores formation which allows the efflux of ions, thereby ensuring efficient killing of target bacteria.
Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin, People's Republic of China.
Han-Sheng
HS
Gong
Xiang-Chen
XC
Meng
Hui
H
Wang
D000255:Adenosine Triphosphate / Q000032:analysis; D001430:Bacteriocins / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects; D048191:Lactiplantibacillus plantarum / Q000378:metabolism*; D008564:Membrane Potentials / Q000187:drug effects*; D010710:Phosphates / Q000032:analysis; D011188:Potassium / Q000032:analysis; D018892:Proton-Motive Force; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000648:ultrastructure
D001430:Bacteriocins; D010710:Phosphates; C557557:plantaricin MG; D000255:Adenosine Triphosphate; D011188:Potassium