Pectin lyase production by recombinant Penicillium griseoroseum strain 105.
Recombinant Penicillium griseoroseum strain 105 overproduces an extracellular pectin lyase (PL) under the transcriptional control of the strong gpdA promoter of Aspergillus nidulans. Our aim was to evaluate PL production by recombinant P. griseoroseum strain 105 in submerged fermentation system bioreactors BioFloIII and BioFloIV using 2 or 10 L working volumes under different growth conditions and to analyze the production of cellulase, polygalacturonase, pectin methylesterase, and protease. PL overproduction by recombinant P. griseoroseum strain 105 was 112 times higher than that of P. griseoroseum PG63 grown in sugarcane juice. Cellulases and proteases were not detected in the culture filtrate, and evaluation for extracellular proteins in the culture medium by SDS-PAGE showed the presence of a 36 kDa predominant band, similar to the molecular mass estimated from the nucleotide sequence of plg1 gene for PL of P. griseoroseum strain 105. This recombinant strain provides the advantage of PL production, which predominates over other extracellular proteins usually present in most commercial pectinase preparations, using sugarcane juice as a substrate of low cost.
Departamento de Biologia, Universidade Federal de Lavras, MG, Brazil.
Patrícia Gomes
PG
Cardoso
Janaina Aparecida
JA
Teixeira
Marisa Vieira
MV
de Queiroz
Elza Fernandes
EF
de Araújo
D001233:Aspergillus nidulans / Q000235:genetics*; D019149:Bioreactors; D002265:Carboxylic Ester Hydrolases / Q000096:biosynthesis; D002480:Cellulase / Q000096:biosynthesis; D004274:DNA, Recombinant; D004591:Electrophoresis, Polyacrylamide Gel; D005285:Fermentation / Q000235:genetics; D019649:Food Industry; D015870:Gene Expression; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D030781:Organisms, Genetically Modified; D010407:Penicillium / Q000201:enzymology* / Q000235:genetics*; D010447:Peptide Hydrolases / Q000096:biosynthesis; D011096:Polygalacturonase / Q000096:biosynthesis; D011133:Polysaccharide-Lyases / Q000096:biosynthesis*; D013379:Substrate Specificity; D013783:Textile Industry
D004274:DNA, Recombinant; D002265:Carboxylic Ester Hydrolases; C022323:pectinesterase; D011096:Polygalacturonase; D002480:Cellulase; D010447:Peptide Hydrolases; D011133:Polysaccharide-Lyases; C027349:pectin lyase