Optical melting measurements of nucleic acid thermodynamics.
Optical melting experiments provide measurements of thermodynamic parameters for nucleic acids. These thermodynamic parameters are widely used in RNA structure prediction programs and DNA primer design software. This review briefly summarizes the theory and underlying assumptions of the method and provides practical details for instrument calibration, experimental design, and data interpretation.
Department of Chemistry and Biochemistry, University of Oklahoma, Norman, Oklahoma, USA.
Susan J
SJ
Schroeder
Douglas H
DH
Turner
D009690:Nucleic Acid Conformation; D012313:RNA / Q000737:chemistry*; D012984:Software; D013816:Thermodynamics
D012313:RNA