Immunogenicity of a DNA vaccine of Avian Reovirus orally delivered by attenuated Salmonella typhimurium.
This study aimed to assess the ability of a Salmonella typhimurium-mediated Avain Reovirus DNA vaccine in eliciting antibody production. Six-day-old SPF chickens were orally immunized with SL7207 (pVAX-σC) twice at 2-week interval, detectable antibody was generated 2 weeks after immunization and was significantly higher than the control groups (P<0.01) and ten chickens (66.7%) were considered safe in the subsequent challenge. These results show that SL7207 (pVAX-σC) can induce protective antibody in chickens and the newly-constructed vaccine is also effective in protection chickens against ARV infection.
Laboratory of Animal Infectious Disease and Microarray, Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China.
Junjie
J
Wan
Cunwei
C
Wang
Xintian
X
Wen
Xiaobo
X
Huang
ShanShan
S
Ling
Yong
Y
Huang
Sanjie
S
Cao
D000284:Administration, Oral; D000818:Animals; D002645:Chickens; D030101:Orthoreovirus, Avian / Q000276:immunology*; D011201:Poultry Diseases / Q000517:prevention & control* / Q000821:virology; D012088:Reoviridae Infections / Q000517:prevention & control / Q000662:veterinary*; D012486:Salmonella typhimurium / Q000235:genetics*; D013047:Specific Pathogen-Free Organisms; D014613:Vaccines, Attenuated / Q000276:immunology; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology*
D014613:Vaccines, Attenuated; D019444:Vaccines, DNA; D014765:Viral Vaccines