Characterization, genetic diversity, and evolutionary link of Cucumber mosaic virus strain New Delhi from India.
The genome of Cucumber mosaic virus New Delhi strain (CMV-ND) from India, obtained from tomato, was completely sequenced and compared with full genome sequences of 14 known CMV strains from subgroups I and II, for their genetic diversity. Sequence analysis suggests CMV-ND shares maximum sequence identity at the nucleotide level with a CMV strain from Taiwan. Among all 15 strains of CMV, the encoded protein 2b is least conserved, whereas the coat protein (CP) is most conserved. Sequence identity values and phylogram results indicate that CMV-ND belongs to subgroup I. Based on the recombination detection program result, it appears that CMV is prone to recombination, and different RNA components of CMV-ND have evolved differently. Recombinational analysis of all 15 CMV strains detected maximum recombination breakpoints in RNA2; CP showed the least recombination sites.
Advanced Centre for Plant Virology, Division of Plant Pathology, Indian Agricultural Research Institute, New Delhi, India.
Vikas
V
Koundal
Qazi Mohd Rizwanul
QM
Haq
Shelly
S
Praveen
D000595:Amino Acid Sequence; D001483:Base Sequence; D005075:Biological Evolution; D017799:Cucumovirus / Q000235:genetics*; D005814:Genes, Viral; D014644:Genetic Variation; D007194:India; D018551:Solanum lycopersicum / Q000821:virology; D008958:Models, Molecular; D008969:Molecular Sequence Data; D058974:Phylogeography; D011995:Recombination, Genetic; D016415:Sequence Alignment; D017421:Sequence Analysis; D014764:Viral Proteins / Q000235:genetics
D014764:Viral Proteins