Contamination of commercial PCR master mix with DNA from Coxiella burnetii.
Contamination of an in-house diagnostic real-time PCR for Q fever was traced back to a commercially obtained PCR Master Mix. It was established that this Master Mix contained DNA from Coxiella burnetii, probably as a result of the use of compounds of animal origin such as bovine serum albumin.
Tilburg
Jeroen J H C
JJ
Department of Medical Microbiology and Infectious Diseases, Canisius Wilhelmina Hospital, and Radboud University Nijmegen Medical Center, Weg door Jonkerbos 100, 6532 SZ Nijmegen, Netherlands.
Nabuurs-Franssen
Marrigje H
MH
van Hannen
Erik J
EJ
Horrevorts
Alphons M
AM
Melchers
Willem J G
WJ
Klaassen
Corné H W
CH
D002021:Buffers; D016997:Coxiella burnetii / Q000235:genetics*; D004247:DNA / Q000032:analysis* / Q000235:genetics; D006801:Humans; D016133:Polymerase Chain Reaction; D011933:Reagent Kits, Diagnostic
D002021:Buffers; D011933:Reagent Kits, Diagnostic; D004247:DNA