Surface display of respiratory syncytial virus glycoproteins in Lactococcus lactis NZ9000.
OBJECTIVE
A system for displaying heterologous respiratory syncytial virus (RSV) glycoproteins on the surface of Lactococcus lactis NZ9000 was developed.


RESULTS
Fusion of the USP45 signal peptide and the cA (C terminus of the peptidoglycan-binding) domains of AcmA, a major autolysin from L. lactis, to the N- and C-terminal of the target proteins, respectively, was carried out. The target protein was the major immunogenic domain of either the F (40.17-kDa) or G (11.49-kDa) glycoprotein domains of the RSV. Whole-cell ELISA readings obtained after 24 h of induction showed an increase in protein expression as the cA domain repeats increased, for the G glycoprotein of RSV. On the other hand, the F glycoprotein indicated decreasing expression levels as the number of cA domain repeats increased. The difference in the expression levels of the F and G domains may be attributed to the different sizes of the antigenic domains.


CONCLUSIONS
The size and properties of the target proteins are vital in determining the amount of antigenic domains being displayed on the surface of live cells.


CONCLUSIONS
The system demonstrated here can aid in the utilization of the generally regarded as safe (GRAS) bacteria L. lactis, as a vaccine delivery vehicle to surface display the antigenic proteins of RSV.
Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Selangor, Malaysia.
S H E
SH
Lim
F
F
Jahanshiri
R Abdul
RA
Rahim
Z
Z
Sekawi
K
K
Yusoff
D000956:Antigens, Viral / Q000378:metabolism; D003001:Cloning, Molecular; D005818:Genetic Engineering; D013294:Lactococcus lactis / Q000235:genetics / Q000378:metabolism*; D021382:Protein Sorting Signals; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012136:Respiratory Syncytial Viruses / Q000235:genetics* / Q000378:metabolism; D014170:Transformation, Genetic; D014760:Viral Fusion Proteins / Q000378:metabolism*
D000956:Antigens, Viral; C526850:F protein, human respiratory syncytial virus; C502275:G glycoprotein, Respiratory syncytial virus; D021382:Protein Sorting Signals; D011993:Recombinant Fusion Proteins; D014760:Viral Fusion Proteins