Comparing dendritic with linear esterase peptides by screening SPOT arrays for catalysis.
Fluorescence screening of a 96-membered SPOT library of histidine containing dendritic and linear peptides revealed the remarkable esterolytic activity of short histidine oligomers that show catalytic proficiencies within one order of magnitude of histidine-containing esterase peptide dendrimers.
Biswas
Rasomoy
R
Department of Chemistry and Biochemistry, University of Berne, Freiestrasse 3, CH-3012 Berne, Switzerland.
Maillard
Noélie
N
Kofoed
Jacob
J
Reymond
Jean-Louis
JL
D055162:Biocatalysis; D050091:Dendrimers / Q000737:chemistry*; D004950:Esterases / Q000737:chemistry* / Q000378:metabolism*; D005453:Fluorescence; D006639:Histidine / Q000737:chemistry; D006868:Hydrolysis; D015394:Molecular Structure; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism; D040081:Protein Array Analysis
D050091:Dendrimers; D010455:Peptides; D006639:Histidine; D004950:Esterases