Interaction-dependent PCR: identification of ligand-target pairs from libraries of ligands and libraries of targets in a single solution-phase experiment.
Interaction-dependent PCR (IDPCR) is a solution-phase method to identify binding partners from combined libraries of small-molecule ligands and targets in a single experiment. Binding between DNA-linked targets and DNA-linked ligands induces formation of an extendable duplex. Extension links codes that identify the ligand and target into one selectively amplifiable DNA molecule. In a model selection, IDPCR resulted in the enrichment of DNA encoding all five known protein-ligand pairs out of 67 599 possible sequences.
McGregor
Lynn M
LM
Department of Chemistry and Chemical Biology and Howard Hughes Medical Institute, Harvard University, 12 Oxford Street, Cambridge, Massachusetts 02138, USA.
Gorin
David J
DJ
Dumelin
Christoph E
CE
Liu
David R
DR
D001665:Binding Sites; D004247:DNA / Q000737:chemistry*; D008024:Ligands; D016133:Polymerase Chain Reaction; D011485:Protein Binding; D054852:Small Molecule Libraries / Q000737:chemistry; D012996:Solutions / Q000737:chemistry
D008024:Ligands; D054852:Small Molecule Libraries; D012996:Solutions; D004247:DNA