Identification of tetranectin as a potential biomarker for metastatic oral cancer.
Lymph node involvement is the most important predictor of survival rates in patients with oral squamous cell carcinoma (OSCC). A biomarker that can indicate lymph node metastasis would be valuable to classify patients with OSCC for optimal treatment. In this study, we have performed a serum proteomic analysis of OSCC using 2-D gel electrophoresis and liquid chromatography/tandem mass spectrometry. One of the down-regulated proteins in OSCC was identified as tetranectin, which is a protein encoded by the CLEC3B gene (C-type lectin domain family 3, member B). We further tested the protein level in serum and saliva from patients with lymph-node metastatic and primary OSCC. Tetranectin was found significantly under-expressed in both serum and saliva of metastatic OSCC compared to primary OSCC. Our results suggest that serum or saliva tetranectin may serve as a potential biomarker for metastatic OSCC. Other candidate serum biomarkers for OSCC included superoxide dismutase, ficolin 2, CD-5 antigen-like protein, RalA binding protein 1, plasma retinol-binding protein and transthyretin. Their clinical utility for OSCC detection remains to be further tested in cancer patients.
Arellano-Garcia
Martha E
ME
School of Dentistry and Dental Research Institute, University of California Los Angeles, Los Angeles, CA, USA; E-Mails: martha_eliz6@yahoo.com (M.E.A.-G.); liuliuxj@ucla.edu (X.L.).
Li
Roger
R
Liu
Xiaojun
X
Xie
Yongming
Y
Yan
Xiaofei
X
Loo
Joseph A
JA
Hu
Shen
S
D014408:Biomarkers, Tumor / Q000032:analysis / Q000097:blood*; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis*; D016022:Case-Control Studies; D006801:Humans; D037181:Lectins, C-Type / Q000032:analysis / Q000097:blood*; D008207:Lymphatic Metastasis / Q000175:diagnosis; D009062:Mouth Neoplasms / Q000175:diagnosis*; D012463:Saliva / Q000737:chemistry
D014408:Biomarkers, Tumor; D037181:Lectins, C-Type; C048648:tetranectin