Complete genomic sequence of transmissible gastroenteritis virus TS and 3' end sequence characterization following cell culture.
The complete genome sequence of transmissible Gastroenteritis virus (TGEV) strain TS, previously isolated from Gansu province, was cloned and compared with published sequence data from other TGEV strains. Phylogenetic tree analysis based on the amino acid and nucleotide sequences of the S gene showed that the TGEV strains were divided into 3 clusters. TGEV TS showed a close evolutionary relationship to the American Miller cluster but had a 5' non-translated region (NTR) sequence closely related to the American Purdue cluster. Continued culture in different cell types indicated that TGEV TS virulence could be attenuated after fifty passages in Porcine kidney (PK-15) cells, and that the Porcine kidney cell line IB-RS-2 (IBRS) was not suitable for culture of the TGEV strain TS.
Li
Jian-qiang
JQ
Key laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.
Cheng
Jie
J
Lan
Xi
X
Li
Xue-rui
XR
Li
Wei
W
Yin
Xiang-ping
XP
Li
Bao-yu
BY
Yang
Bin
B
Li
Zhi-yong
ZY
Zhang
Yun
Y
Liu
Ji-xing
JX
D020413:3' Untranslated Regions; D000818:Animals; D002460:Cell Line; D016000:Cluster Analysis; D016679:Genome, Viral; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D012692:Serial Passage; D013552:Swine; D005760:Transmissible gastroenteritis virus / Q000235:genetics* / Q000472:pathogenicity; D014774:Virulence
D020413:3' Untranslated Regions; D012367:RNA, Viral