Ex vivo viability of canine and feline sarcomas: a pilot study.
Assay-based chemotherapeutic protocols are common in human gynecologic oncology, most notably for patients with ovarian or breast cancer. The current study examines ex vivo incubation conditions necessary for the assessment of sarcomatous tumor response to potential chemotherapeutic drugs. Slices of sarcomatous tumors were incubated in one of two culture media. Viability indices were measured and compared across time and between media. Neither medium was sufficient to support the growth of sarcomatous tumor tissue slices based on the indices studied. It is likely that sarcomatous tumors require a different approach for ex vivo assessment than their epithelial counterparts. Our long-term goal is to incubate tumor slices with chemotherapeutic agents to predict the in vivo tumor response based on the maintenance or loss of slice viability within this system.
Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Auburn University, Auburn, AL 36849, USA.
Nicki
N
Green
Dawn Merton
DM
Boothe
Annette
A
Smith
Ralph
R
Henderson
Elizabeth
E
Whitley
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002371:Cat Diseases / Q000473:pathology*; D002415:Cats; D004283:Dog Diseases / Q000473:pathology*; D004285:Dogs; D010865:Pilot Projects; D012509:Sarcoma / Q000378:metabolism*; D046509:Tissue Culture Techniques / Q000662:veterinary*
D000255:Adenosine Triphosphate