Induction of heat shock proteins in the adult rat cerebral cortex following pilocarpine-induced status epilepticus.
The expression pattern of a global set of Hsps in the adult rat cerebral cortex was examined during the first week following pilocarpine-induced status epilepticus (SE). Hsp70 and Hsp32 protein were induced at 1 day post-SE and decreased thereafter, whereas Hsp25 exhibited a prolonged induction with increased levels at 3 and 7 days post-SE. Hsp70 was induced in neurons, while Hsp25 and Hsp32 localized to GFAP-positive hypertrophied astrocytes. The induction of Hsp25, Hsp32 and Hsp70 at 1 day following SE suggests the involvement of these Hsps in early events after SE. Interestingly, while Hsp32 and Hsp70 expression was transient, Hsp25 demonstrated a sustained induction pattern, which may reflect an additional role of Hsp25 in subsequent remodeling events in the days following SE.
Lively
Starlee
S
Center for the Neurobiology of Stress, University of Toronto Scarborough, Toronto, Ontario, Canada.
Brown
Ian R
IR
D000818:Animals; D001253:Astrocytes / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism*; D055551:HSP27 Heat-Shock Proteins / Q000378:metabolism*; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D006419:Heme Oxygenase (Decyclizing) / Q000378:metabolism*; D008297:Male; D009474:Neurons / Q000378:metabolism; D010862:Pilocarpine; D051381:Rats; D017208:Rats, Wistar; D013226:Status Epilepticus / Q000139:chemically induced / Q000378:metabolism*; D013997:Time Factors
D055551:HSP27 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; C486114:Hspb1 protein, rat; D010862:Pilocarpine; D006419:Heme Oxygenase (Decyclizing); C491523:Hmox1 protein, rat