Metabolite formation of 17alpha-hydroxyprogesterone as a consequence of e⁻(aq)-emission and progesterone effect regarding cancer.
Based on previous investigations on several hormones, 17α-hydroxyprogesterone (17α-HOPRG) was studied in respect to cancer initiation by its metabolites resulting from electron emission. The emission of electrons (e⁻(aq)) from its singlet excited state of 17α-HOPRG and HPLC-analysis of products were studied. Possible carcinogenicity of metabolites originating from 17α-HOPRG and the effect of progesterone (PRG) in this respect were studied in vitro. The results showed that 17α-HOPRG is very sensitive towards oxygen. The highest Q(e⁻(aq)) values were obtained by dissolution and UV-irradiation of substrate in airfree media. 17α-HOPRG metabolites showed a strong anticancer activity, which is, however, lower compared to that of PRG-metabolites. Mixture of both hormones, 17α-HOPRG and PRG, in respect to carcinogenicity showed a synergistic effect of PRG on 17α-HOPRG. Reaction mechanisms are presented.
Getoff
Nikola
N
Section of Radiation Biology, Faculty of Life Sciences, University of Vienna, Althanstr.14, UZA IIA-1090 Vienna, Austria. nikola.getoff@univie.ac.at
Danielova
Iren
I
Hartmann
Johannes
J
Schittl
Heike
H
Gerschpacher
Marion
M
Ying
Shaobin
S
Quint
Ruth
R
Huber
Johannes C
JC
D004583:Electrons / Q000009:adverse effects / Q000627:therapeutic use*; D005609:Free Radicals / Q000737:chemistry / Q000378:metabolism / Q000528:radiation effects; D006801:Humans; D066298:In Vitro Techniques; D008956:Models, Chemical; D009369:Neoplasms / Q000532:radiotherapy*; D009381:Neoplasms, Radiation-Induced / Q000209:etiology*; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism; D011374:Progesterone / Q000031:analogs & derivatives* / Q000737:chemistry* / Q000378:metabolism / Q000528:radiation effects; D014466:Ultraviolet Rays / Q000009:adverse effects; D014467:Ultraviolet Therapy / Q000379:methods
D005609:Free Radicals; C002175:progesterone 17 alpha-hydroperoxide; D011374:Progesterone; D010100:Oxygen