Sequence analysis of the gag-pol gene of human immunodeficiency virus type 1 of intersubtype (B'/C) recombinant strain in Beijing, China.
Little is known about the molecular and biological properties of HIV-1 intersubtype B'/C in Beijing. To fill the gap, we sequenced and analyzed the gag-pol genes from 39 HIV-1 B'/C recombinant infectors in Beijing, China during 2007. The results show that 36 CRF07_BC and 2 CRF08_BC isolates have a structural profile identical or nearly identical to CRF07_BC or CRF08_BC according to sequences in the gag-pol regions. The CRF07_BC circulating in injecting drug users (IDUs) and heterosexuals forms a diverse phylogenetic tree and most isolates from homosexuals cluster together. However, all the B'/C recombinant strains were remarkable for their low interpatient diversity in gag-pol genes (3.1, 3.0, and 2.2% for isolates from IDUs, heterosexuals, and homosexuals, respectively). We identified I7V, E91G, N242T, and K361R in the gag gene and R290I (HXB2 positions) in the pol gene as signature amino acid substitutions characteristic of HIV-1 CRF07_BC from the Beijing lineage. In addition, one new B'/C recombinant was detected. These results may contribute to an understanding of HIV-1 in Beijing.
Ye
Jing-rong
JR
National Institute for Viral Disease Control and Prevention, China Center for Disease Prevention and Control, Beijing, China.
Xin
Ruo-lei
RL
Bai
Li-shi
LS
Lu
Hong-yan
HY
Yu
Shuang-qin
SQ
Zeng
Yi
Y
D000328:Adult; D002681:China; D004279:DNA, Viral / Q000235:genetics; D055030:Drug Users; D005260:Female; D015750:Genes, gag / Q000235:genetics*; D015751:Genes, pol / Q000235:genetics*; D014644:Genetic Variation; D015497:HIV-1 / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D020010:Heterosexuality; D006716:Homosexuality; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D055815:Young Adult
D004279:DNA, Viral