Intrinsic defects in B cell response to seasonal influenza vaccination in elderly humans.
We have evaluated the serum response to seasonal influenza vaccination in subjects of different ages and associated this with the specific B cell response to the vaccine in vitro. Although the serum response has previously been shown to decrease with age, this has largely been associated to decreased T cell functions. Our results show that in response to the vaccine, the specific response of B cells in vitro, as measured by AID (activation-induced cytidine deaminase), the in vivo serum HI (hemagglutination inhibition) response, and the in vivo generation of switch memory B cells are decreased with age, as evaluated in the same subjects. This is the first report to demonstrate that intrinsic B cell defects with age contribute to reduced antibody responses to the influenza vaccine. The level of AID in response to CpG before vaccination can also predict the robustness of the vaccine response. These results could contribute to developing more effective vaccines to protect the elderly as well as identifying those most at risk.
Department of Microbiology and Immunology, University of Miami Miller School of Medicine, Miami, FL 33101, USA.
Daniela
D
Frasca
Alain
A
Diaz
Maria
M
Romero
Ana Marie
AM
Landin
Mitch
M
Phillips
Suzanne C
SC
Lechner
John G
JG
Ryan
Bonnie B
BB
Blomberg
D000328:Adult; D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D000914:Antibodies, Viral / Q000097:blood; D001402:B-Lymphocytes / Q000276:immunology*; D003564:Cytidine Deaminase / Q000378:metabolism; D005260:Female; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D007156:Immunologic Memory; D007252:Influenza Vaccines / Q000276:immunology*; D007251:Influenza, Human / Q000517:prevention & control*; D008297:Male; D008875:Middle Aged; D000098830:AICDA (Activation-Induced Cytidine Deaminase)
D000914:Antibodies, Viral; D007252:Influenza Vaccines; D000098830:AICDA (Activation-Induced Cytidine Deaminase); D003564:Cytidine Deaminase