Study on effect of peptide-conjugated near-infrared fluorescent quantum dots on the clone formation, proliferation, apoptosis, and tumorigenicity ability of human buccal squamous cell carcinoma cell line BcaCD885.
Quantum dots (QDs) have shown great development potential in noninvasive imaging and monitoring of cancer cells in vivo because of their unique optical properties. However, the key issue of whether or not QDs-labeled cancer cells affect the proliferation, apoptosis and in vivo tumorigenicity ability has not been reported. The primary issue is if the results obtained from the noninvasive visualization of QDs-labeled tumors are scientific. Here, we applied peptide-linked near-conjugated fluorescent QDs to label human buccal squamous cell carcinoma cell line (BcaCD885). We performed in vivo tumorigenicity ability assays, tumorigenic cells proliferation, and apoptotic capability assays detected by flow cytometry and plate clone formation experiment, and found that peptide-linked near-conjugated fluorescent QDs labeling did not affect the growth, proliferation, apoptosis, and tumorigenicity ability of those cancer cells. Our study provides scientific foundation to support the application of near-infrared fluorescent QDs in noninvasive imaging and monitoring of cancer cells in vivo.
Department of Oral and Maxillofacial Surgery, First Affiliated Hospital, Chongqing Medical University, Chongqing, China.
Deping
D
Sun
Kai
K
Yang
Gang
G
Zheng
Zhigang
Z
Li
Yuan
Y
Cao
D000818:Animals; D017209:Apoptosis; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002610:Cheek; D005260:Female; D005453:Fluorescence; D006801:Humans; D007259:Infrared Rays; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009062:Mouth Neoplasms / Q000473:pathology*; D050997:Nanomedicine; D009368:Neoplasm Transplantation; D010455:Peptides; D045663:Quantum Dots; D014183:Transplantation, Heterologous; D014410:Tumor Stem Cell Assay
D010455:Peptides