Genome analysis of Bifidobacterium bifidum PRL2010 reveals metabolic pathways for host-derived glycan foraging.
The human intestine is densely populated by a microbial consortium whose metabolic activities are influenced by, among others, bifidobacteria. However, the genetic basis of adaptation of bifidobacteria to the human gut is poorly understood. Analysis of the 2,214,650-bp genome of Bifidobacterium bifidum PRL2010, a strain isolated from infant stool, revealed a nutrient-acquisition strategy that targets host-derived glycans, such as those present in mucin. Proteome and transcriptome profiling revealed a set of chromosomal loci responsible for mucin metabolism that appear to be under common transcriptional control and with predicted functions that allow degradation of various O-linked glycans in mucin. Conservation of the latter gene clusters in various B. bifidum strains supports the notion that host-derived glycan catabolism is an important colonization factor for B. bifidum with concomitant impact on intestinal microbiota ecology.
Laboratory of Probiogenomics, Department of Genetics, Biology of Microorganisms, Anthropology and Evolution, University of Parma, 43100 Parma, Italy.
Francesca
F
Turroni
Francesca
F
Bottacini
Elena
E
Foroni
Imke
I
Mulder
Jae-Han
JH
Kim
Aldert
A
Zomer
Borja
B
Sánchez
Alessandro
A
Bidossi
Alberto
A
Ferrarini
Vanessa
V
Giubellini
Massimo
M
Delledonne
Bernard
B
Henrissat
Pedro
P
Coutinho
Marco
M
Oggioni
Gerald F
GF
Fitzgerald
David
D
Mills
Abelardo
A
Margolles
Denise
D
Kelly
Douwe
D
van Sinderen
Marco
M
Ventura
D001644:Bifidobacterium / Q000235:genetics* / Q000254:growth & development; D005243:Feces / Q000382:microbiology; D020869:Gene Expression Profiling; D016680:Genome, Bacterial; D023281:Genomics / Q000379:methods; D054884:Host-Pathogen Interactions / Q000235:genetics*; D006801:Humans; D007231:Infant, Newborn; D007422:Intestines / Q000382:microbiology; D053858:Metabolic Networks and Pathways / Q000235:genetics*; D008969:Molecular Sequence Data; D009077:Mucins / Q000378:metabolism; D005810:Multigene Family; D011134:Polysaccharides / Q000378:metabolism*
D009077:Mucins; D011134:Polysaccharides