Effect of antibiotic treatment on bacteriophage production by a cystic fibrosis epidemic strain of Pseudomonas aeruginosa.
Phage production in response to antibiotics varied among four isolates of a Pseudomonas aeruginosa cystic fibrosis (CF) epidemic strain. Whereas ciprofloxacin induced higher levels of phage production, other CF-relevant antibiotics led to reduced production. We detected free phages directly in CF patient sputum samples by both plaque (40% positive) and PCR (76% positive) assays. Our observations suggest that the choice of antibiotics could influence the number of free phages within the CF lung environment.
Fothergill
Joanne L
JL
NIHR Biomedical Research Centre in Microbial Disease, Royal Liverpool University Hospital, Liverpool, United Kingdom.
Mowat
Eilidh
E
Walshaw
Martin J
MJ
Ledson
Martin J
MJ
James
Chloe E
CE
Winstanley
Craig
C
D001435:Bacteriophages / Q000187:drug effects* / Q000235:genetics; D003550:Cystic Fibrosis / Q000382:microbiology*; D006801:Humans; D016133:Polymerase Chain Reaction; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000472:pathogenicity / Q000821:virology*