[Effects of IL-4 and taxol on cellular proliferation of Lewis lung cancer].
BACKGROUND
To investigate the effects of IL-4 and taxol on cellular proliferation of Lewis lung cancer (LLC) .


METHODS
LLC cells (1. 25 *10(6)/ mouse) were inoculated subcutaneously to 40 mice , which were randomized into 4 groups and given responsive treatment consecutively in 5 days after inoculation. For group I natrii chloride was injected , for group II IL-4 at 50 ng/ d for 7 days , for group III taxol at 10 mg/ (kg*d) for 10 days , and for group IV IL-4 and taxol were injected altogether at 50 ng/ d for 7 days and 5mg/ (kg*d) for 10 days respectively. On the 18th day , all the mice were killed. Nuclear morphometry , DNA contents and AgNORs were detected by image analysis.


RESULTS
Nuclear area , perimeter , mean diameter , DNA contents , and AgNORs counting in group II were 24. 202 +/-5. 012mum(2) , 18. 626 +/-4. 180mum , 2. 642 +/-0. 291mum , 3. 412 +/-0. 910 , and 5. 313 +/-0. 820 respectively , which were significantly less than those in group I ( P < 0. 05) ; the parameters in group III were 18. 373 +/-4. 833mum(2) , 16. 522 +/-2. 518mum , 2. 401 +/-0. 288mum , 3. 096 +/-0. 771 , and 2. 670 +/-0. 401 respectively , which were much less than those in group I ( P < 0. 01) ; the parameters in group IV were 16. 664 +/-4. 761mum(2) , 15. 901 +/-2. 700mum , 2. 283 +/-0. 308mum , 2. 707 +/-0. 807 , and 1. 552 +/-0. 316 respectively , which were remarkably less than those in group I ( P < 0. 01) . Significant differences of the parameters were observed among group II and III and IV ( P < 0. 05) .


CONCLUSIONS
Both IL-4 and taxol can remarkably inhibit the proliferation of LLC. The effect of combined treatment is most significant , and the effect of taxol is higher than that of IL-4.
Department of Pathology , Hubei Medical University , Wuhan , Hubei 430071 , P. R. China.
X
X
Hu
M
M
Liu
L
L
Yu
D
D
Chen
M
M
Jiang
X
X
Liu
D
D
Xia
Z
Z
Zhang