[Application of multiple polymorphism genetic markers in determination of half sibling sharing a same mother].
OBJECTIVE
Determination strategies for half sibling sharing a same mother were investigated through the detection of autosomal and X-chromosomal STR (X-STR) loci and polymorphisms on hypervariable (HV) region of mitochondrial DNA (mtDNA).


METHODS
Genomic DNA were extracted from blood stain samples of the 3 full siblings and one dubious half sibling sharing the same mother with them. Fifteen autosomal STR loci were genotyped by Sinofiler kit, and 19 X-STR loci were genotyped by Mentype Argus X-8 kit and 16 plex in-house system. Polymorphisms of mtDNA HV-I and HV-II were also detected with sequencing technology.


RESULTS
Full sibling relationship between the dubious half sibling and each of the 3 full siblings were excluded based on the results of autosomal STR genotyping and calculation of full sibling index (FSI) and half sibling index (HIS). Results of sequencing for mtDNA HV-I and HV-II showed that all of the 4 samples came from a same maternal line. X-STR genotyping results determined that the dubious half sibling shared a same mother with the 3 full siblings.


CONCLUSIONS
It is reliable to combine three different genotyping technologies including autosomal STR, X-STR and sequencing of mtDNA HV-I and HV-II for determination of half sibling sharing a same mother.
Que
Ting-zhi
TZ
Shanghai Key Laboratory of Forensic Medicine, Institute of Forensic Science, Ministry of Justice, PR China, Shanghai 200063, China. quetz@ssfjd.com.cn
Zhao
Shu-min
SM
Li
Cheng-tao
CT
D041321:Chromosomes, Human, X / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics*; D005260:Female; D053803:Forensic Genetics / Q000379:methods; D005819:Genetic Markers; D005838:Genotype; D006801:Humans; D008297:Male; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA; D035781:Siblings; D020080:Tandem Repeat Sequences / Q000235:genetics*
D004272:DNA, Mitochondrial; D005819:Genetic Markers