New functional sites in MutS affect DNA mismatch repair.
The MutS protein plays an important role in the DNA mismatch repair system. Mutations in the mutS gene can lead to genome instability and ultimately cell malfunction. Here we have established a method for identifying functional defective mutants of MutS by random mutation and rifampicin screening. Some novel functional sites in MutS were identified. The MutS mutant strains were analyzed using surface plasmon resonance, gel filtration and far-western methods to determine the molecular mechanisms behind the DNA mismatch repair function of MutS.
Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101, China.
Tianying
T
Zhong
Lijun
L
Bi
Xianen
X
Zhang
D000251:Adenosine Triphosphatases / Q000378:metabolism; D001665:Binding Sites / Q000235:genetics; D026481:Blotting, Far-Western; D002850:Chromatography, Gel; D053843:DNA Mismatch Repair / Q000235:genetics* / Q000502:physiology*; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D005798:Genes, Bacterial; D000070956:MutL Proteins; D051719:MutS DNA Mismatch-Binding Protein / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D016296:Mutagenesis; D050505:Mutant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D054730:Protein Interaction Domains and Motifs; D055503:Protein Multimerization; D020349:Surface Plasmon Resonance
D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; C074770:MutL protein, E coli; D050505:Mutant Proteins; D000251:Adenosine Triphosphatases; D000070956:MutL Proteins; D051719:MutS DNA Mismatch-Binding Protein; C497175:MutS protein, E coli