Recombinant pseudorabies virus expressing P12A and 3C of FMDV can partially protect piglets against FMDV challenge.
One of the crucial factors for evaluation of an effective genetically engineered vaccine is whether susceptible animals are protected from virus challenge after vaccination. In this study, a recombinant pseudorabies virus (PRV-P12A3C) that expressed capsid precursor polypeptide P12A and nonstructural protein 3C of foot-and-mouth disease virus (FMDV) was used as a vaccine. The expression of P12A3C and its immunogenicity and protective efficacy against FMDV challenge were measured. Humoral and cellular immune responses were evaluated after each immunization. Subsequently, each piglet was challenged with 1000 ID(50) (50% infection dose) FMDV serotype O, named OR/80, which is used to produce vaccine in China. PRV-P12A3C induced a high level of neutralizing antibody and FMDV-specific lymphocytes. Inactivated vaccine provided 100% protection, and the vector strain (TK(-)/gE(-)/gI(-)) showed no protection. PRV-P12A3C induced 60% protection, compared with piglets that were vaccinated with TK(-)/gE(-)/gI(-). The severity of clinical signs for the remaining two piglets was lighter and the appearance of vesicles was delayed.
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, Hubei, PR China; Lanzhou Veterinary Research Institute of Chinese Academy of Agriculture Science, State Key Laboratory of Veterinary Etiological Biology, National Foot-and-Mouth Disease Reference Laboratory, Key Laboratory of Animal Virology of Ministry of Agriculture, Xujiaping No. 1, Yanchangpu, Lanzhou, Gansu 730046, PR China.
Keshan
K
Zhang
Institute of Veterinary Medicine, Xinjiang Academy of Animal Science, Urumqi 830000, China.
Jiong
J
Huang
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, Hubei, PR China.
Qingang
Q
Wang
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, Hubei, PR China.
Yannan
Y
He
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, Hubei, PR China.
Zhuofei
Z
Xu
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, Hubei, PR China.
Min
M
Xiang
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, Hubei, PR China. Electronic address: wub@mail.hzau.edu.cn.
Bin
B
Wu
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, Hubei, PR China.
Huanchun
H
Chen
D000086042:3C Viral Proteases; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D036022:Capsid Proteins / Q000008:administration & dosage / Q000276:immunology*; D003546:Cysteine Endopeptidases / Q000276:immunology*; D019084:Fluorescent Antibody Technique, Indirect / Q000662:veterinary; D005536:Foot-and-Mouth Disease / Q000276:immunology* / Q000517:prevention & control; D011558:Herpesvirus 1, Suid / Q000276:immunology*; D007111:Immunity, Cellular; D056724:Immunity, Humoral; D008213:Lymphocyte Activation; D010849:Picornaviridae / Q000276:immunology; D013552:Swine; D013553:Swine Diseases / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000276:immunology; D014764:Viral Proteins / Q000276:immunology*; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology*
D000914:Antibodies, Viral; D036022:Capsid Proteins; D014614:Vaccines, Synthetic; D014764:Viral Proteins; D014765:Viral Vaccines; D003546:Cysteine Endopeptidases; D000086042:3C Viral Proteases