In vitro biomimetic construction of hydroxyapatite-porcine acellular dermal matrix composite scaffold for MC3T3-E1 preosteoblast culture.
The application of porous hydroxyapatite-collagen (HAp-Collagen) as a bone tissue engineering scaffold is hindered by two main problems: its high cost and low initial strength. As a native 3-dimenssional collagen framework, purified porcine acellular dermal matrix (PADM) has been successfully used as a skin tissue engineering scaffold. Here we report its application as a matrix for the preparation of HAp to produce a bone tissue scaffold through a biomimetic chemical process. The HAp-PADM scaffold has two-level pore structure, with large channels (∼100 μm in diameter) inherited from the purified PADM microstructure and small pores (<100 nm in diameter) formed by self-assembled HAp on the channel surfaces. The obtained HAp-PADM scaffold (S15D) has a compressive elastic modulus as high as 600 kPa. The presence of HAp in sample S15D reduces the degradation rate of PADM in collagenase solution at 37°C. After 7 day culture of MC3T3-E1 pre-osteroblasts, MTT data show no statistically significant difference on pure PADM framework and HAp-PADM scaffold (p > 0.05). Because of its high strength and nontoxicity, its simple preparation method, and designable and tailorable properties, the HAp-PADM scaffold is expected to have great potential applications in medical treatment of bone defects.
State Key Laboratory of Crystal Materials, Center of Bio and Micro/Nano Functional Materials, School of Physics and Microelectronics, Shandong University, Jinan, PR China.
Hongshi
H
Zhao
Guancong
G
Wang
Shunpeng
S
Hu
Jingjie
J
Cui
Na
N
Ren
Duo
D
Liu
Hong
H
Liu
Chengbo
C
Cao
Jiyang
J
Wang
Zhonglin
Z
Wang
D000818:Animals; D001673:Biodegradation, Environmental / Q000187:drug effects; D001696:Biomechanical Phenomena / Q000187:drug effects; D040761:Biomimetic Materials / Q000494:pharmacology*; D001826:Body Fluids / Q000378:metabolism; D002113:Calcification, Physiologic / Q000187:drug effects; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D020405:Dermis / Q000166:cytology*; D017886:Durapatite / Q000494:pharmacology*; D005109:Extracellular Matrix / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D051379:Mice; D018613:Microscopy, Confocal; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D016062:Porosity / Q000187:drug effects; D034421:Sus scrofa; D054457:Tissue Scaffolds / Q000737:chemistry*; D014961:X-Ray Diffraction
D017886:Durapatite