Thrombogenicity of sirolimus-eluting stents and bare metal stents: evaluation in the early phase after stent implantation.
BACKGROUND
Thrombogenicitiy of drug-eluting stents is a matter of controversial debate. The aim of this study was to evaluate the thrombogenicity of sirolimus-eluting stents (SES) compared to bare metal stents (BMS) in a standardised in vitro model.


METHODS
Nine SES and nine BMS were implanted in tubing loops and nine loops without stent served as controls. Initially and after 90 minutes of blood circulation in a modified chandler loop model, thrombin-antithrombin III complex (TAT), PMN-elastase, factor XIIa, SC5b-9, sP-selectin and platelet count were measured. Expression of CD62P, CD45/41 and PAC-1 on platelets were determined by flow cytometry.


RESULTS
After 90 minutes, platelet count decreased significantly in the loops with BMS and SES (p<0.05). Levels of TAT, PMN-elastase and SC5b-9 were significantly elevated after 90 minutes in all loops (p<0.05). sP-selectin significantly increased in the loops with BMS and SES after 90 minutes. No significant changes occurred in any flow cytometric data. Platelet count, sP-selectin, TAT, PMN-elastase, SC5b-9, CD62P, CD41/CD45 and PAC-1 showed no significant difference between BMS and SES.


CONCLUSIONS
These data provide evidence that there is no difference in thrombogenicity of BMS and SES in the in vitro model.
Walter
Thomas
T
University Medical Centre Mannheim, First Department of Medicine (Cardiology), Theodor-Kutzer-Ufer 1-3, D-68167 Mannheim, Germany. thomas.walter.med@umm.de
Rey
Katja S
KS
Wendel
Hans Peter
HP
Szabo
Sebastian
S
Suselbeck
Tim
T
Dempfle
Carl-Erik
CE
Borggrefe
Martin
M
Swoboda
Stefanie
S
Beyer
Martin E
ME
Hoffmeister
Hans Martin
HM
D000328:Adult; D015415:Biomarkers; D001792:Blood Platelets / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000378:metabolism; D054855:Drug-Eluting Stents / Q000009:adverse effects*; D005434:Flow Cytometry; D006801:Humans; D007166:Immunosuppressive Agents / Q000009:adverse effects*; D066298:In Vitro Techniques; D008297:Male; D008670:Metals / Q000009:adverse effects*; D008855:Microscopy, Electron, Scanning; D019007:P-Selectin / Q000097:blood; D010974:Platelet Aggregation; D010976:Platelet Count; D020123:Sirolimus / Q000009:adverse effects*; D013927:Thrombosis / Q000139:chemically induced*; D055815:Young Adult
D015415:Biomarkers; D015815:Cell Adhesion Molecules; D007166:Immunosuppressive Agents; D008670:Metals; D019007:P-Selectin; D020123:Sirolimus