Screening of a high growth influenza B virus strain in Vero cells.
Due to the insufficient supply of embryonated chicken eggs, the preparation of large quantities of inactivated influenza vaccines will require an alternative virus culture system after the emergence or reemergence of a pandemic influenza virus. The Vero cell is one of the ideal options since it was used for producing many kinds of human vaccines. However, most of the influenza viruses can not grow well in Vero cells. To develop a new influenza vaccine with Vero cells as a substrate, the virus needs to adapt to this cell substrate to maintain high growth characteristics. By serial passages in Vero cells, the B/Yunnan/2/2005va (B) strain was successfully adapted to Vero cells, with the hemagglutination titer (HAT) of the virus reaching 1:512. The high growth characteristic of this strain is stable up to 21 passages. The strain was identified by hemagglutination inhibition (HAI) test and sequencing respectively; the HA₁ gene sequence of the virus was cloned and analyzed. The screening and establishment of high growth B virus provides an important tool for influenza vaccine production in Vero cells.
Liu
Ze
Z
Institute of Medical Biology, Chinese Academy of Medical Sciences, Kunming 650118, China.
Li
Wei-dong
WD
Sun
Ming-bo
MB
Ma
Lei
L
Guo
Zi-quan
ZQ
Jiang
Shu-de
SD
Liao
Guo-yang
GY
Yang
Jing-si
JS
Li
Chang-gui
CG
D000220:Adaptation, Biological; D000818:Animals; D002522:Chlorocebus aethiops; D016000:Cluster Analysis; D006385:Hemagglutination Inhibition Tests; D009981:Influenza B virus / Q000235:genetics / Q000254:growth & development*; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D012692:Serial Passage; D014709:Vero Cells; D019562:Viral Load