[Cloning of full-length coding sequence of tree shrew CD3E and prediction of its molecular characteristics].
The use of tree shrews (Tupaia belangeri) in human disease studies demands essential research tools, in particular cellular markers and their monoclonal antibodies for immunological studies. Here we cloned the full-length cDNAs encoding CD3E from total RNA of the spleen, liver and peripheral blood of tree shrews and analyzed their structural characteristics in comparison with other mammals by Discovery Studio software. The results showed that the open reading frame sequence of tree shrew CD3E was 582 bp, encoding 194 amino acids. The overall structure of tree shrew CD3E protein was similar to its counterparts of other mammals, intracellular and transmembrane domain highly conserved. However, detailed analysis revealed two potential glycosylation sites and different surface charges in the extracellular domain. Availability of the entire open-reading-frame and related sequence information would therefore facilitate the preparation of monoclonal antibodies against tree shrew CD3 and further studies for its function.
Li
Yi-Jiang
YJ
College of Animal Science and Veterinary Medicine, Yunnan Agricultural University, Kunming Yunnan, China. liyijiang88.student@sina.com
Gao
Yue-Dong
YD
Guo
Yan
Y
Lu
Cai-Xia
CX
Huang
Jing-Fei
JF
Xia
Xue-Shan
XS
Dai
Jie-Jie
JJ
Fan
Quan-Shui
QS
Li
Zuo-Sheng
ZS
Zhang
Hua-Tang
HT
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017252:CD3 Complex / Q000737:chemistry / Q000235:genetics*; D003001:Cloning, Molecular; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010802:Phylogeny; D014418:Tupaiidae / Q000276:immunology*
D017252:CD3 Complex