Effects of citric acid on cultured human osteoblastic cells.
We investigated the effects of citric acid (CA) on cultured human osteoblastic (HOB) cells by evaluating cell adhesion, proliferation, and cytotoxicity. (3)H-Thymidine-labeled HOB cells were incubated in culture medium supplemented or not with 4%, 6%, 8%, or 10% CA for 1 minute. After incubation, cell morphology was evaluated by Nomarski interferential light microscopy, cell proliferation was accessed by measurements of (3)H-thymidine associated to the cells, and cell lysis was monitored by measuring the amount of (3)H-thymidine released by cells. We observed that most of the CA-treated cells presented numerous atypical vacuoles, and such cells were also highly polymorphic, exhibiting round-shaped cells. Nonetheless, CA at all concentrations assayed did not yield cytotoxicity as measured by (3)H-containing DNA release, although significant decrease in cell proliferation was observed (P > .05). Furthermore, cells which were treated with CA at the lowest concentration assayed (4%) restored normal proliferation rates 3 days after treatment.
Department of Pediatric Dentistry and Orthodontics, School of Dentistry, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
Lizandra Ferrari
LF
Guimarães
Tatiana Kelly da Silva
TK
Fidalgo
Gustavo Conde
GC
Menezes
Laura Guimarães
LG
Primo
Fernando
F
Costa e Silva-Filho
D002448:Cell Adhesion / Q000187:drug effects; D018929:Cell Culture Techniques; D016923:Cell Death / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002478:Cells, Cultured; D019343:Citric Acid / Q000494:pharmacology* / Q000633:toxicity; D003470:Culture Media; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008857:Microscopy, Interference; D010006:Osteoblasts / Q000187:drug effects*; D011554:Pseudopodia / Q000187:drug effects; D019275:Radiopharmaceuticals; D013936:Thymidine; D013997:Time Factors; D014316:Tritium; D014617:Vacuoles / Q000187:drug effects
D003470:Culture Media; D019275:Radiopharmaceuticals; D014316:Tritium; D019343:Citric Acid; D013936:Thymidine