Preparation of PEI-coated bacterial biosorbent in water solution: optimization of manufacturing conditions using response surface methodology.
The aim of this study is to optimize preparation method of polyethyleneimine (PEI)-coated bacterial biosorbent in water as reaction media using fermentation waste biomass of Corynebacterium glutamicum as a raw material. The fermentation waste biomass of C. glutamicum and Reactive Red 4 were used as model raw bacterium and pollutant. Major factors affecting the performance of PEI-coated biosorbent were the amounts of polymer (PEI) and cross-linker glutaraldehyde (GA). These factors were optimized through response surface methodology (RSM) with two-level-two-factor (2(2)) full factorial central composite design. As a result, the optimum conditions were found to be 4.29 g of PEI and 0.15 mL of GA, with 10 g of the biomass, where the sorption capacity was enhanced 4.52-fold compared to that of the raw biomass. Therefore, this simple, cost-effective, and water-based method could be a useful modification tool for the development of a high performance biosorbent for removing anionic pollutants.
Department of Bioprocess Engineering, Chonbuk National University, Jeonbuk 561-756, Republic of Korea.
Juan
J
Mao
In-Seob
IS
Kwak
Muthuswamy
M
Sathishkumar
Krishnamurthy
K
Sneha
Yeoung-Sang
YS
Yun
D000327:Adsorption; D000704:Analysis of Variance; D001673:Biodegradation, Environmental; D018533:Biomass; D048230:Corynebacterium glutamicum / Q000737:chemistry*; D007700:Kinetics; D008956:Models, Chemical; D011094:Polyethyleneimine / Q000737:chemistry*; D012044:Regression Analysis; D012996:Solutions; D013499:Surface Properties; D013696:Temperature; D014867:Water / Q000737:chemistry*; D018508:Water Purification / Q000379:methods*
D012996:Solutions; D014867:Water; D011094:Polyethyleneimine