Attenuation of allergic airway inflammation and hyperresponsiveness in a murine model of asthma by silver nanoparticles.
The use of silver in the past demonstrated the certain antimicrobial activity, though this has been replaced by other treatments. However, nanotechnology has provided a way of producing pure silver nanoparticles, and it shows cytoprotective activities and possible pro-healing properties. But, the mechanism of silver nanoparticles remains unknown. This study was aimed to investigate the effects of silver nanoparticles on bronchial inflammation and hyperresponsiveness. We used ovalbumin (OVA)-inhaled female C57BL/6 mice to evaluate the roles of silver nanoparticles and the related molecular mechanisms in allergic airway disease. In this study with an OVA-induced murine model of allergic airway disease, we found that the increased inflammatory cells, airway hyperresponsiveness, increased levels of IL-4, IL-5, and IL-13, and the increased NF-κB levels in lungs after OVA inhalation were significantly reduced by the administration of silver nanoparticles. In addition, we have also found that the increased intracellular reactive oxygen species (ROS) levels in bronchoalveolar lavage fluid after OVA inhalation were decreased by the administration of silver nanoparticles. These results indicate that silver nanoparticles may attenuate antigen-induced airway inflammation and hyperresponsiveness. And antioxidant effect of silver nanoparticles could be one of the molecular bases in the murine model of asthma. These findings may provide a potential molecular mechanism of silver nanoparticles in preventing or treating asthma.
Division of Pulmonology, Allergy and Critical Care Medicine, Department of Internal Medicine, Chungnam National University Medical School, Daejeon, Korea.
Hee Sun
HS
Park
Keun Hwa
KH
Kim
Sunhyae
S
Jang
Ji Won
JW
Park
Hye Rim
HR
Cha
Jeong Eun
JE
Lee
Ju Ock
JO
Kim
Sun Young
SY
Kim
Choong Sik
CS
Lee
Joo Pyung
JP
Kim
Sung Soo
SS
Jung
D000818:Animals; D001249:Asthma / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology / Q000503:physiopathology; D001483:Base Sequence; D004195:Disease Models, Animal; D005260:Female; D007249:Inflammation / Q000188:drug therapy / Q000473:pathology; D007378:Interleukins / Q000235:genetics; D053768:Metal Nanoparticles / Q000008:administration & dosage / Q000627:therapeutic use* / Q000648:ultrastructure; D051379:Mice; D008810:Mice, Inbred C57BL; D046529:Microscopy, Electron, Transmission; D050997:Nanomedicine; D010047:Ovalbumin / Q000276:immunology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012130:Respiratory Hypersensitivity / Q000188:drug therapy / Q000503:physiopathology; D012834:Silver / Q000008:administration & dosage / Q000627:therapeutic use*; D051996:Transcription Factor RelA / Q000378:metabolism
D007378:Interleukins; D012333:RNA, Messenger; C498144:Rela protein, mouse; D051996:Transcription Factor RelA; D012834:Silver; D010047:Ovalbumin