REST corepressor (CoREST) repression induces phenotypic gene regulation in advanced osteoarthritic chondrocytes.
Alternations in cartilage chondrocyte phenotype characteristic by the decreased type II collagen and aggrecan together with increased type X collagen synthesis serve as a beacon for osteoarthritis progression. However, little is known about the underlying molecular mechanisms. The current study seeks to discover molecules that involved in osteoarthritic chondrocytes phenotype regulation. Differential proteomics was generated with two-dimensional gel electrophoresis between normal articular cartilage (NAC) and advanced osteoarthritic cartilage (AOC). Those differentially expressed proteins were identified by mass spectrometry. The down-regulation of a neuronal silencer, the REST corepressor (CoREST) in AOC, was verified by Western blot. CoREST silencing was performed in primarily cultured NAC chondrocytes with specific siRNA to reveal the possible involvement of CoREST repression in chondrocyte phenotypic genes modulation. Ninteen differentially expressed proteins were screened and identified. Among these proteins, CoREST, HHL, and zinc finger protein 155 were estimated to be possible gene modulators. CoREST protein level was verified to be down-regulated by 69.5% (p < 0.001) in AOC. In response to CoREST knock-down by 64.8% (p < 0.001) in NAC chondrocytes, the gene expression level of the chondrocyte terminal differentiation marker gene, collagen X was found to be up-regulated by 40.0% (p = 0.017), whereas the chondrocyte differentiation phenotypic genes, collagen II and aggrecan were down-regulated by 71.4% (p < 0.001) and 57.6% (p < 0.001), respectively. The results indicate that the silencing of CoREST by siRNA transfection in NAC may reflect CoREST repression in AOC, which results in phenotypic genes modulation and suggests a homeostatic role of this transcription factor in articular chondrocyte.
Department of Orthopaedics and Traumatology, Peking Union Medical College Hospital, PUMC, Beijing, China.
Jun
J
Xiao
Tao
T
Li
Zhihong
Z
Wu
Zhanjun
Z
Shi
Jianting
J
Chen
Stephen K L
SK
Lam
Zandong
Z
Zhao
Lanbo
L
Yang
Guixing
G
Qiu
D000328:Adult; D000368:Aged; D053674:Aggrecans / Q000096:biosynthesis; D002358:Cartilage, Articular / Q000378:metabolism; D019902:Chondrocytes / Q000378:metabolism*; D056970:Co-Repressor Proteins; D024043:Collagen Type II / Q000096:biosynthesis; D024121:Collagen Type X / Q000096:biosynthesis; D015536:Down-Regulation; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D008297:Male; D008875:Middle Aged; D009419:Nerve Tissue Proteins / Q000502:physiology*; D010003:Osteoarthritis / Q000235:genetics* / Q000378:metabolism*; D010641:Phenotype; D012097:Repressor Proteins / Q000502:physiology*; D015854:Up-Regulation
D053674:Aggrecans; D056970:Co-Repressor Proteins; D024043:Collagen Type II; D024121:Collagen Type X; D009419:Nerve Tissue Proteins; C121019:RCOR1 protein, human; D012097:Repressor Proteins