[Tissue integration of various silicone-coated polypropylene surgical mesh].
OBJECTIVE
Laparoscopic ventral hernia repair requires a surgical mesh implanted in intraperitoneal position. The combined, double layer meshes are promising in animal models as well as in human practice. The aim of this study was to compare the biological behaviour of two different textured silicone covered polypropylene mesh.


METHODS
3 × 4 cm big full thickness defect of the abdominal wall was created in New Zealand White rabbits. The defect was covered in 20 animals with a polypropylene mesh with laminar silicone layer on the visceral surface (LSPP), while the remaining 20 cases the defects were covered with a macroporous textured silicone impregnated polypropylene mesh (MSPP). Intraperitoneal adhesion formation and tissue ingrowth in the meshes were investigated. Immunohistochemistry was used to detect proliferation activity (Ki-67), neovascularization (VEGF), and to visualize mesothelial layer (CK) over the mesh. Scanning electron microscopy was used to investigate the visceral surface of the meshes.


RESULTS
While intraperitoneal adhesion formation showed no difference after 1 week, LSPP mesh induced significantly less adhesions after 21 days. The Ki-67 positivity was significantly lower and the number of the VEGF positive cells increased with time in the MSPP group, this was missing in the LSPP group. The thin neoperitoneum layer was detected over MSPP mesh only with CK antibody.


CONCLUSIONS
The material and texture of the mesh are responsible for tissular incorporation which is in accordance with the generated foreign body reaction.
Takács
Ildikó
I
Pécsi Tudományegyetem, Általános Orvostudományi Kar Sebészeti Oktató és Kutató Intézet, 7624 Pécs, Kodály Zoltán u. 20. takacsildi@yahoo.com
Horváth
Szabolcs
S
Balatonyi
Borbála
B
Jávor
Szaniszló
S
Molnár
Agnes
A
Gáspár
Sarolta
S
Hajós
Rebeka
R
Meczker
Agnes
A
Lantos
János
J
Roth
Erzsébet
E
Wéber
György
G
D034861:Abdominal Wall / Q000601:surgery*; D000818:Animals; D001672:Biocompatible Materials; D002448:Cell Adhesion; D049109:Cell Proliferation; D005549:Foreign-Body Reaction / Q000503:physiopathology; D046449:Hernia, Abdominal / Q000601:surgery; D007150:Immunohistochemistry; D007633:Keratins / Q000032:analysis; D019394:Ki-67 Antigen / Q000032:analysis; D008855:Microscopy, Electron, Scanning; D023421:Models, Animal; D018919:Neovascularization, Physiologic; D010537:Peritoneum / Q000502:physiology*; D011126:Polypropylenes; D011817:Rabbits; D012828:Silicones; D013526:Surgical Mesh; D023822:Tissue Engineering / Q000379:methods; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis
D001672:Biocompatible Materials; D019394:Ki-67 Antigen; D011126:Polypropylenes; D012828:Silicones; D042461:Vascular Endothelial Growth Factor A; D007633:Keratins