Inhibition of two enzyme systems in Euchlanis dilatata (Rotifera: Monogononta) as biomarker of effect of metals and pesticides.
The inhibitory effects on esterases and phospholipase A2 (PLA2) in the freshwater rotifer Euchlanis dilatata, native to Mexico, were assessed by fluorimetry after in vivo exposure (30 min) in laboratory conditions to sublethal concentrations of metals and pesticides. EC(50) values for esterases ranged from 7.9 × 10(-7) for DDT to 61.9 μg l(-1) for methyl parathion, while corresponding values for PLA2 ranged from 0.96 × 10(-6) for mercury to 69.2 μg l(-1) for lead. These enzyme systems in E. dilatata are very sensitive to the tested agents and suggest they would be suitable biomarkers. However, sensitivity to other environmental contaminants should be investigated in laboratory conditions and field studies to assess their potential as environmental biomarkers.
Arias-Almeida
Juan Carlos
JC
Universidad Nacional Autónoma de México, Posgrado en Ciencias del Mar y Limnología, Ciudad Universitaria, Mexico. jcariasa@hotmail.com
Rico-Martínez
Roberto
R
D000825:Animal Structures / Q000187:drug effects / Q000201:enzymology; D000818:Animals; D000831:Animals, Newborn; D055162:Biocatalysis / Q000187:drug effects; D015415:Biomarkers / Q000378:metabolism; D002104:Cadmium / Q000494:pharmacology / Q000633:toxicity; D003634:DDT / Q000494:pharmacology / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology* / Q000633:toxicity; D004798:Enzymes / Q000378:metabolism*; D004950:Esterases / Q000037:antagonists & inhibitors / Q000378:metabolism; D005456:Fluorescent Dyes / Q000378:metabolism; D007854:Lead / Q000494:pharmacology / Q000633:toxicity; D007928:Lethal Dose 50; D008627:Mercuric Chloride / Q000494:pharmacology / Q000633:toxicity; D019216:Metals, Heavy / Q000494:pharmacology* / Q000633:toxicity; D008743:Methyl Parathion / Q000494:pharmacology / Q000633:toxicity; D010575:Pesticides / Q000494:pharmacology* / Q000633:toxicity; D064801:Phospholipase A2 Inhibitors; D054467:Phospholipases A2 / Q000378:metabolism; D012403:Rotifera / Q000187:drug effects* / Q000201:enzymology*; D014877:Water Pollution, Chemical / Q000032:analysis*
D015415:Biomarkers; D004791:Enzyme Inhibitors; D004798:Enzymes; D005456:Fluorescent Dyes; D019216:Metals, Heavy; D010575:Pesticides; D064801:Phospholipase A2 Inhibitors; D002104:Cadmium; D007854:Lead; D008743:Methyl Parathion; D008627:Mercuric Chloride; D003634:DDT; D004950:Esterases; D054467:Phospholipases A2