Transgenerational (in utero/lactational) exposure to investigate the effects of endocrine disrupting compounds (EDCS) in rats.
This protocol is designed to evaluate the effects of endocrine disrupting compounds (EDCs) through fetal (transplacental) and/or neonatal (via the dam's milk) exposure during the critical periods of reproductive organogenesis in the rat. Continued direct exposure to the F1 pups after weaning is an option that can also be included. Reproductive indices, growth, and viability are monitored throughout the life of the F1 generation and an extensive necropsy is conducted after sexual maturity is attained. The endpoints evaluated are designed to detect compounds such as androgen receptor antagonists or steroid hormone synthesis inhibitors which inhibit normal development of the male reproductive system and growth stimulating hormones which masculinize females and facilitate development of male reproductive tissues. Data collected from these studies can be used to determine the no and lowest observed effects levels and predict potential adverse consequences of EDCs in human and wildlife populations. The information also provides a foundation for future studies designed to identify mechanism(s) of action, specific genes and or proteins involved, and susceptible populations these compounds might target.
Ostby
Joseph S
JS
United States Environmental Protection Agency, Research Triangle Park, North Carolina, USA.
Gray
L Earl
LE
D000726:Androgen Antagonists / Q000633:toxicity; D000728:Androgens / Q000633:toxicity; D000818:Animals; D000831:Animals, Newborn; D052244:Endocrine Disruptors / Q000633:toxicity*; D005260:Female; D007774:Lactation / Q000378:metabolism*; D008098:Litter Size; D008297:Male; D018811:Maternal Exposure; D008431:Maternal-Fetal Exchange; D018812:Paternal Exposure; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects / Q000209:etiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012741:Sexual Maturation
D000726:Androgen Antagonists; D000728:Androgens; D052244:Endocrine Disruptors