Detecting CD20-rituximab specific interactions on lymphoma cells using atomic force microscopy.
Elucidating the underlying mechanisms of cell physiology is currently an important research topic in life sciences. Atomic force microscopy methods can be used to investigate these molecular mechanisms. In this study, single-molecule force spectroscopy was used to explore the specific recognition between the CD20 antigen and anti-CD20 antibody Rituximab on B lymphoma cells under near-physiological conditions. The CD20-Rituximab specific binding force was measured through tip functionalization. Distribution of CD20 on the B lymphoma cells was visualized three-dimensionally. In addition, the relationship between the intramolecular force and the molecular extension of the CD20-Rituximab complex was analyzed under an external force. These results facilitate further investigation of the mechanism of Rituximab's anti-cancer effect.
Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang, 110016, China.
Mi
M
Li
LianQing
L
Liu
Ning
N
Xi
YueChao
Y
Wang
ZaiLi
Z
Dong
GuangYong
G
Li
XiuBin
X
Xiao
WeiJing
W
Zhang
D058846:Antibodies, Monoclonal, Murine-Derived / Q000378:metabolism*; D000936:Antigen-Antibody Complex / Q000378:metabolism; D018951:Antigens, CD20 / Q000378:metabolism*; D000970:Antineoplastic Agents / Q000378:metabolism; D001665:Binding Sites; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000276:immunology / Q000378:metabolism; D006801:Humans; D016393:Lymphoma, B-Cell / Q000276:immunology* / Q000378:metabolism*; D018625:Microscopy, Atomic Force; D008856:Microscopy, Fluorescence; D000069283:Rituximab
D058846:Antibodies, Monoclonal, Murine-Derived; D000936:Antigen-Antibody Complex; D018951:Antigens, CD20; D000970:Antineoplastic Agents; D000069283:Rituximab