Generation and analysis of ESTs from the grass carp, Ctenopharyngodon idellus.
Grass carp, Ctenopharyngodon idellus (Valenciennes, 1844), is an economically important species widely cultured in the world, but its genome research resources are largely lacking. The objectives of this study were to construct normalized cDNA libraries for efficient EST analysis, to generate ESTs from these libraries, and to identify EST-related molecular markers such as microsatellites and single nucleotide polymorphisms (SNPs) for genetic analysis of this species. A total of 6,269 ESTs were generated representing 4,815 unique sequences, from which 105 putative microsatellites and 5,228 SNPs were identified. These genome resources provide the material basis for future genetic and functional analyses in this species.
The Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Education, Shanghai, China.
Bing
B
Xu
Shaolin
S
Wang
Yan
Y
Jiang
Linlin
L
Yang
Ping
P
Li
Caixia
C
Xie
Jubin
J
Xing
Zhonghe
Z
Ke
Jiale
J
Li
Junwei
J
Gai
Guimei
G
Yang
Baolong
B
Bao
Zhanjiang
Z
Liu
D000818:Animals; D001483:Base Sequence; D002347:Carps / Q000235:genetics*; D003001:Cloning, Molecular; D020224:Expressed Sequence Tags; D015723:Gene Library; D005819:Genetic Markers; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide; D012313:RNA / Q000737:chemistry / Q000302:isolation & purification; D013045:Species Specificity
D005819:Genetic Markers; D012313:RNA