Mesenchymal stem cells improve the healing of ischemic colonic anastomoses (experimental study).
OBJECTIVE
The goal of this study is to examine if allogenic mesenchymal stem cell (MSC) transplantation is a useful therapy for left ischemic colon anastomosis in rats. Problems with anastomosis healing may lead to serious postoperative complications. Bone marrow-derived mesenchymal stem cells (BM-MSCs), which are also referred to as stromal progenitor cells, are self-renewing and expandable stem cells. Recent studies have suggested that BM-MSCs play a crucial role in the processes of intestinal repair and accelerate angiogenesis.


METHODS
MSCs were isolated from rats before analysis by light and scanning electron microscopy. Forty male Wistar albino rats weighing 250-280 g were divided into four equal groups (n = 10) as follows: group 1: control, ischemic left colonic anastomoses (fourth day); group 2: control, ischemic left colonic anastomoses (seventh day); group 3: ischemic left colonic anastomoses + locally transplanted BM-MSCs (fourth day); group 4: ischemic left colonic anastomoses + locally transplanted BM-MSCs (seventh day). Histopathological features and anastomotic strength were evaluated.


RESULTS
BM-MSCs therapy significantly accelerated all of the healing parameters for ischemic colonic anastomosis except for inflammation on the fourth day. On the seventh day, BM-MSCs augmented the levels of the hydroxyproline and bursting pressure. Histological parameters, especially angiogenesis, were also found to be important for healing of ischemic colonic anastomoses.


CONCLUSIONS
This is the first study to use locally transplanted cell therapy for the healing of ischemic colonic anastomosis. BM-MSCs therapy significantly accelerated all of the healing parameters for ischemic colonic anastomosis.
Adas
Gokhan
G
Department of Surgery, Okmeydani Education and Research Hospital, Istanbul, Turkey.
Arikan
Soykan
S
Karatepe
Oguzhan
O
Kemik
Ozgur
O
Ayhan
Selda
S
Karaoz
Erdal
E
Kamali
Gulcin
G
Eryasar
Bahar
B
Ustek
Duran
D
D000714:Anastomosis, Surgical; D000818:Animals; D003106:Colon / Q000098:blood supply* / Q000473:pathology / Q000601:surgery*; D004195:Disease Models, Animal; D007511:Ischemia / Q000473:pathology / Q000601:surgery*; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D008855:Microscopy, Electron, Scanning; D051381:Rats; D017208:Rats, Wistar; D014945:Wound Healing / Q000502:physiology*