A cDNA microarray approach for analyzing transcriptional changes in Penaeus monodon after infection by pathogens.
A cDNA microarray comprised of 9990 different ESTs obtained from the Penaeus monodon EST project (http://pmonodon.biotec.or.th) was employed to identify viral (white spot and yellow head viruses) and bacterial (Vibrio harveyi) responsive genes in the hemocytes of P. monodon at 6, 24 and 48 h post-injection (hpi). The number of differentially expressed genes found was highest in shrimps infected with white spot virus (1954 genes) followed by yellow head virus (1136 genes) and V. harveyi (420 genes). Changes in shrimp gene expression were highest at the late infection stage for both viruses, whilst that for V. harveyi induced gene expression was mainly found at the early infection stage, but the repression of genes was mainly found in the mid stage of infection. Shrimp genes specifically upregulated by each particular pathogen are identified and are summarized.
Pongsomboon
Siriporn
S
Department of Biochemistry, Faculty of Science, Chulalongkorn University, Center of Excellence for Molecular Biology and Genomics of Shrimp, 254 Phayathai Road, Bangkok 10330, Thailand.
Tang
Sureerat
S
Boonda
Suleeporn
S
Aoki
Takashi
T
Hirono
Ikuo
I
Tassanakajon
Anchalee
A
D000818:Animals; D001692:Biological Transport; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism; D004247:DNA / Q000096:biosynthesis; D004734:Energy Metabolism / Q000502:physiology; D020869:Gene Expression Profiling; D006706:Homeostasis; D054884:Host-Pathogen Interactions; D020411:Oligonucleotide Array Sequence Analysis; D033561:Penaeidae / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology* / Q000502:physiology; D015398:Signal Transduction; D014158:Transcription, Genetic / Q000276:immunology*; D015854:Up-Regulation; D014733:Vibrio / Q000502:physiology*
D018797:Cell Cycle Proteins; D004247:DNA