Fabrication of chitosan/silk fibroin composite nanofibers for wound-dressing applications.
Chitosan, a naturally occurring polysaccharide with abundant resources, has been extensively exploited for various biomedical applications, typically as wound dressings owing to its unique biocompatibility, good biodegradability and excellent antibacterial properties. In this work, composite nanofibrous membranes of chitosan (CS) and silk fibroin (SF) were successfully fabricated by electrospinning. The morphology of electrospun blend nanofibers was observed by scanning electron microscopy (SEM) and the fiber diameters decreased with the increasing percentage of chitosan. Further, the mechanical test illustrated that the addition of silk fibroin enhanced the mechanical properties of CS/SF nanofibers. The antibacterial activities against Escherichia coli (Gram negative) and Staphylococcus aureus (Gram positive) were evaluated by the turbidity measurement method; and results suggest that the antibacterial effect of composite nanofibers varied on the type of bacteria. Furthermore, the biocompatibility of murine fibroblast on as-prepared nanofibrous membranes was investigated by hematoxylin and eosin (H&E) staining and MTT assays in vitro, and the membranes were found to promote the cell attachment and proliferation. These results suggest that as-prepared chitosan/silk fibroin (CS/SF) composite nanofibrous membranes could be a promising candidate for wound healing applications.
Key Laboratory of Textile Science & Technology, Ministry of Education, Donghua University, Shanghai, 201620, China; E-Mail: caizengxiao@sina.com.
Zeng-Xiao
ZX
Cai
Xiu-Mei
XM
Mo
Kui-Hua
KH
Zhang
Lin-Peng
LP
Fan
An-Lin
AL
Yin
Chuang-Long
CL
He
Hong-Sheng
HS
Wang
D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology; D001458:Bandages; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D002460:Cell Line; D048271:Chitosan / Q000737:chemistry*; D005347:Fibroblasts / Q000187:drug effects; D005349:Fibroins / Q000737:chemistry* / Q000494:pharmacology; D051379:Mice; D057139:Nanofibers / Q000737:chemistry*; D013211:Staphylococcus aureus / Q000187:drug effects
D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D005349:Fibroins; D048271:Chitosan