Mouse granulosa cells contribute more to the mRNA synthesis of mZP2 than oocyte does.
In order to elucidate the biogenesis of mouse zona pellucida 2 (mZP2) protein, RT-PCR, and in situ hybridization were carried out to localize the expression of mouse ZP2 mRNA. Cumulus cells of the OCC (Oocyte-Cumulus cell Complex) were isolated from the oocytes after superovulation for the RNA extraction. The frozen sections of ovaries from adolescent and aged mice were prepared to hybridize with RNA probe of mouse ZP2. mRNA of ZP2 was detected in isolated cumulus cells by RT-PCR. Results of in situ hybridization showed that the mRNA of ZP2 was synthesized in both oocyte and granulosa cells at different folliculogenesis stages; and the expression of ZP2 mRNA in granulosa cells was stronger than that in oocyte; much weaker expression of mZP2 was detected in the follicles of aged mouse. These suggest that the entire amount of ZP2 mRNA generated in the granulosa cells layer should be much more than that in oocyte. Therefore, we think that the granulosa cells contribute more to the mZP2 mRNA synthesis than oocyte does.
Institute of Reproductive Immunology, Jinan University, Guangzhou 510632, China.
Yan
Y
Xie
Yanhong
Y
Yu
Chuncheng
C
Nie
Zuowu
Z
Cao
D000818:Animals; D054885:Cumulus Cells / Q000502:physiology*; D004527:Egg Proteins / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D008562:Membrane Glycoproteins / Q000096:biosynthesis* / Q000235:genetics; D051379:Mice; D009865:Oocytes / Q000502:physiology*; D012333:RNA, Messenger / Q000096:biosynthesis*; D011956:Receptors, Cell Surface / Q000096:biosynthesis* / Q000235:genetics; D000072216:Zona Pellucida Glycoproteins
D004527:Egg Proteins; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D000072216:Zona Pellucida Glycoproteins; C000606520:Zp2 protein, mouse