Nuclear factor of activated T cells negatively regulates expression of the tumor necrosis factor receptor-related 2 gene in T cells.
Tumor necrosis factor receptor-related 2 (TR2, HVEM or TNFRSF-14) plays an important role in immune responses, however, the mechanisms regulating its expression are unclear. To understand the control of TR2 gene expression, we studied the upstream region of the gene. Gel supershift assays revealed inducible binding of nuclear factor of activated T cells (NFAT) to a putative NFAT site within the TR2 promoter. Furthermore, cotransfection of a dominant negative NFAT construct, or siRNA for NFAT, resulted in increased expression of a TR2 reporter gene. Our findings demonstrate that NFAT negatively regulates TR2 expression in activated T cells.
Kim
Woon-Ki
WK
Department of Biological Science and the Immunomodulation Research Center, University of Ulsan, Ulsan 680-749, Korea.
Sul
Ok-Ju
OJ
Kwak
Jung-Sook
JS
Hur
Hye-Young
HY
Latour
Anne M
AM
Koller
Beverly H
BH
Kwon
Byoung S
BS
Jeong
Choon-Soo
CS
D000818:Animals; D001483:Base Sequence; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism; D002478:Cells, Cultured; D015536:Down-Regulation; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D050778:NFATC Transcription Factors / Q000502:physiology*; D053240:Receptors, Tumor Necrosis Factor, Member 14 / Q000096:biosynthesis*; D013601:T-Lymphocytes / Q000378:metabolism*
D050778:NFATC Transcription Factors; C494007:Nfatc2 protein, mouse; D053240:Receptors, Tumor Necrosis Factor, Member 14; C506029:Tnfrsf14 protein, mouse