Kinetics of circulating antigen 14-3-3 in sera of rabbits firstly infected with Schistosoma japonicum and treated with/without praziquantel.
A sandwich ELISA was developed for the detection of circulating antigen 14-3-3 in the sera of rabbits. Rabbits that were infected with 500 cercariae of Schistosoma japonicum were grouped and the kinetics of 14-3-3 was observed. For the treated group, the 14-3-3 protein could be detected as early as 2-4 weeks postinfection and then its levels rose rapidly and reached a peak at around 6 weeks. The 14-3-3 levels in the sera significantly decreased after the infected rabbits were treated with praziquantel at 6 weeks postinfection and declined to the initial level about 8 weeks posttreatment. While in the untreated group, 14-3-3 levels reached a peak in 8 weeks postinfection and then remained at plateau level for about 6 weeks. Our findings showed that detection of S. japonicum 14-3-3 has an important value for diagnosis of acute infection of S. japonicum and evaluation of chemotherapy.
Jiangsu Institute of Parasitic Diseases, 117 Yangxiang, Meiyuan, Wuxi, 214064, Jiangsu Province, China.
Chun-Yan
CY
Qian
Li-Jun
LJ
Song
Wan-Quan
WQ
Hua
Xu-Ren
XR
Yin
Jie
J
Wang
Wei
W
Zhang
Yong-Liang
YL
Xu
Chuan-Xin
CX
Yu
D000818:Animals; D000871:Anthelmintics / Q000494:pharmacology*; D000947:Antigens, Helminth / Q000097:blood*; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D008297:Male; D011223:Praziquantel / Q000494:pharmacology*; D011817:Rabbits; D012549:Schistosoma japonicum / Q000187:drug effects* / Q000276:immunology; D012554:Schistosomiasis japonica / Q000097:blood / Q000175:diagnosis / Q000188:drug therapy; D013997:Time Factors
D000871:Anthelmintics; D000947:Antigens, Helminth; D011223:Praziquantel