The non-coding RNA BC1 is down-regulated in the hippocampus of Wistar Audiogenic Rat (WAR) strain after audiogenic kindling.
The aim of this study was to identify molecular pathways involved in audiogenic seizures in the epilepsy-prone Wistar Audiogenic Rat (WAR). For this, we used a suppression-subtractive hybridization (SSH) library from the hippocampus of WARs coupled to microarray comparative gene expression analysis, followed by Northern blot validation of individual genes. We discovered that the levels of the non-protein coding (npc) RNA BC1 were significantly reduced in the hippocampus of WARs submitted to repeated audiogenic seizures (audiogenic kindling) when compared to Wistar resistant rats and to both naive WARs and Wistars. By quantitative in situ hybridization, we verified lower levels of BC1 RNA in the GD-hilus and significant signal ratio reduction in the stratum radiatum and stratum pyramidale of hippocampal CA3 subfield of audiogenic kindled animals. Functional results recently obtained in a BC1⁻/⁻ mouse model and our current data are supportive of a potential disruption in signaling pathways, upstream of BC1, associated with the seizure susceptibility of WARs.
Gitaí
Daniel Leite Goes
DL
Department of Cellular and Molecular Biology, Ribeirão Preto School of Medicine, University of São Paulo, Brazil.
Fachin
Ana Lucia
AL
Mello
Stephano Spanó
SS
Elias
Carol Fuzachi
CF
Bittencourt
Jackson Cioni
JC
Leite
João Pereira
JP
Passos
Geraldo Aleixo da Silva
GA
Garcia-Cairasco
Norberto
N
Paçó-Larson
Maria Luisa
ML
D000161:Acoustic Stimulation / Q000009:adverse effects; D000818:Animals; D004195:Disease Models, Animal; D015536:Down-Regulation / Q000235:genetics*; D004569:Electroencephalography; D020195:Epilepsy, Reflex / Q000235:genetics / Q000473:pathology*; D020869:Gene Expression Profiling / Q000379:methods; D006624:Hippocampus / Q000378:metabolism*; D007696:Kindling, Neurologic / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D020733:RNA, Small Cytoplasmic / Q000235:genetics* / Q000378:metabolism; D022661:RNA, Untranslated / Q000235:genetics*; D051381:Rats; D017208:Rats, Wistar
C117734:BC1 RNA; D020733:RNA, Small Cytoplasmic; D022661:RNA, Untranslated