Multiplexed parallel single transport recordings on nanopore arrays.
We introduce a nanofabricated silicon chip for massively multiplexed analysis of membrane channels and transporters in suspended lipid membranes that does not require any surface modification or organic solvent. Transport processes through single membrane complexes are monitored by fluorescence. The chip consists of an array of well-defined nanopores, addressing an individual pyramidal back-reflecting 30-fL compartment. The setup allows simultaneous analyses of ∼1,000 single transmembrane events in one field of view, observing translocation kinetics of transmembrane complexes.
Institute of Biochemistry, Biocenter, Center for Membrane Proteomics (CMP), and Cluster of Excellence Frankfurt (CEF)-Macromolecular Complexes, Goethe-University Frankfurt, Max-von-Laue-Strasse 9, D-60438 Frankfurt/M., Germany.
Alexander
A
Kleefen
Daniel
D
Pedone
Christian
C
Grunwald
Ruoshan
R
Wei
Matthias
M
Firnkes
Gerhard
G
Abstreiter
Ulrich
U
Rant
Robert
R
Tampé