Expression and immunological analysis of capsid protein precursor of swine vesicular disease virus HK/70.
VP1, a capsid protein of swine vesicular disease virus, was cloned from the SVDV HK/70 strain and inserted into retroviral vector pBABE puro, and expressed in PK15 cells by an retroviral expression system. The ability of the VP1 protein to induce an immune response was then evaluated in guinea pigs. Western blot and ELISA results indicated that the VP1 protein can be recognized by SVDV positive serum, Furthermore, anti-SVDV specific antibodies and lymphocyte proliferation were elicited and increased by VP1 protein after vaccination. These results encourage further work towards the development of a vaccine against SVDV infection.
Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.
Hong
H
Tian
Jing-yan
JY
Wu
You-jun
YJ
Shang
Shuang-hui
SH
Ying
Hai-xue
HX
Zheng
Xiang-tao
XT
Liu
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D015153:Blotting, Western; D036022:Capsid Proteins / Q000235:genetics / Q000276:immunology*; D049109:Cell Proliferation; D003001:Cloning, Molecular; D029822:Enterovirus B, Human / Q000235:genetics / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005822:Genetic Vectors; D006168:Guinea Pigs; D008214:Lymphocytes / Q000276:immunology; D012190:Retroviridae / Q000235:genetics; D014765:Viral Vaccines / Q000276:immunology
D000914:Antibodies, Viral; D036022:Capsid Proteins; D014765:Viral Vaccines