High-throughput SuperSAGE.
SuperSAGE is a method of digital gene expression profiling that allows isolation of 26-bp tag fragments from expressed transcripts. Combined with the ultrahigh-throughput sequencing technologies, SuperSAGE enables analysis of millions of transcripts with lower cost and reduced effort and time. In this chapter, we present an updated protocol for this High-throughput SuperSAGE method with a special emphasis on the technique of library multiplexing.
Iwate Biotechnology Research Center, Kitakami, Iwate, Japan.
Hideo
H
Matsumura
Kentaro
K
Yoshida
Shujun
S
Luo
Detlev H
DH
Krüger
Günter
G
Kahl
Gary P
GP
Schroth
Ryohei
R
Terauchi
D001483:Base Sequence; D017931:DNA Primers; D020869:Gene Expression Profiling; D017422:Sequence Analysis, DNA / Q000379:methods*
D017931:DNA Primers