OVP1, a vacuolar H+-translocating inorganic pyrophosphatase (V-PPase), overexpression improved rice cold tolerance.
Vacuolar H(+)-translocating inorganic pyrophosphatase (V-PPase, EC 3.6.1.1) is an electrogenic proton pump and has been studied in many plants. Here we report characterization of the OVP1 gene from rice (Oryza sativa L.). Quantitative reverse transcriptase-polymerase chain reaction analysis (RT-qPCR) showed that OVP1 was induced by cold stress. OVP1 overexpression resulted in enhanced cold tolerance in transgenic rice, which was related to an increased integrity of cell membrane, decreased MDA content and accumulation of proline to higher level as compared with wild type rice seedlings. These results indicated that V-PPase was an important element in the survival strategies of plants under cold stress.
State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100094, China.
Juan
J
Zhang
Jianqin
J
Li
Xuechen
X
Wang
Jia
J
Chen
D000222:Adaptation, Physiological / Q000235:genetics*; D001692:Biological Transport; D002462:Cell Membrane; D003080:Cold Temperature; D015870:Gene Expression; D043564:Inorganic Pyrophosphatase / Q000235:genetics / Q000378:metabolism*; D015227:Lipid Peroxidation; D008315:Malondialdehyde / Q000378:metabolism; D012275:Oryza / Q000201:enzymology* / Q000235:genetics; D030821:Plants, Genetically Modified; D011392:Proline / Q000378:metabolism*; D011522:Protons; D020133:Reverse Transcriptase Polymerase Chain Reaction; D036226:Seedlings; D014617:Vacuoles / Q000378:metabolism
D011522:Protons; D008315:Malondialdehyde; D011392:Proline; D043564:Inorganic Pyrophosphatase