Preparation and characterization of a porous scaffold based on poly(D,L-lactide) and N-hydroxyapatite by phase separation.
In this study, a series of porous scaffolds were prepared from poly(D,L-lactide) (PLA) and nanohydroxyapatite (HA) using the phase separation method. HA/PLA composite membranes and PLA membranes with a microporous structure (pore size around 10-20 μm) were observed by scanning electron microscopy and these micropores were well distributed throughout the PLA membranes. The surface morphology of HA/PLA composite membranes was significantly improved compared to pure PLA membrane. Also, the mechanical property and contact angle of composite membranes were different from that of pure PLA films. The immortalized rat osteoblastic ROS 17/2.8 cell line was used in this research to study the cell adhesion and proliferation behavior, and the results indicated that composite membranes had great cell affinity and good biocompatibility.
State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University, Chengdu 610041, PR China.
Xiu Hong
XH
Wang
Shuai
S
Shi
Gang
G
Guo
Shao Zhi
SZ
Fu
Min
M
Fan
Feng
F
Luo
Xia
X
Zhao
Yu Quan
YQ
Wei
Zhi Yong
ZY
Qian
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002448:Cell Adhesion; D049109:Cell Proliferation; D017886:Durapatite / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000166:cytology; D011091:Polyesters / Q000737:chemistry*; D016062:Porosity; D051381:Rats; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
D001672:Biocompatible Materials; D011091:Polyesters; C033616:poly(lactide); D017886:Durapatite