The potential of brewer's spent grain to improve the production of α-amylase by Bacillus sp. KR-8104 in submerged fermentation system.
Brewer's spent grain (BSG) was used as a solid substrate for the production of α-amylase by Bacillus sp. KR-8104 in a submerged fermentation system. The production of α-amylase was maximized through statistical optimization of the BSG concentration and incubation time using the Doehlert experimental design. The highest tested amount of BSG (5%, w/v) in the optimization process resulted in a 5.1-fold enhancement of the response. Subsequently, we studied the role of the water-soluble and -insoluble fractions of BSG in the production of α-amylase. The results revealed that whole BSG had a greater effect on the production of α-amylase than each fraction had separately. Finally, when we examined the potential of BSG to replace the constituents of a medium formula, we observed that simultaneously adding BSG, omitting dextrin, and reducing the other ingredients concentration in the culture medium improved the production of α-amylase and made the production process more economical.
Department of Food Science and Engineering, Faculty of Agricultural Engineering and Technology, University of Tehran, Karaj, Iran.
Maryam
M
Hashemi
Seyed Hadi
SH
Razavi
Seyed Abbas
SA
Shojaosadati
Seyyed Mohammad
SM
Mousavi
D000818:Animals; D001407:Bacillus / Q000201:enzymology*; D003470:Culture Media / Q000737:chemistry*; D002523:Edible Grain / Q000737:chemistry*; D005285:Fermentation; D007218:Industrial Microbiology / Q000379:methods*; D008962:Models, Theoretical; D000516:alpha-Amylases / Q000378:metabolism*
D003470:Culture Media; D000516:alpha-Amylases