Enhanced angiogenic capacity of human umbilical vein endothelial cells from women with preeclampsia.
Maternal and placental angiogenic abnormalities are a common feature of preeclampsia. The aim of this study was to determine if endothelial cells from women with preeclampsia exhibit different angiogenic responses compared to healthy cells. Using the endothelial tube formation assay, we have shown that primary human umbilical vein endothelial cells (HUVECs) isolated from women with preeclampsia display greater levels of in vitro angiogenic branching compared to cells from healthy women. A comparable increase in tube formation was observed in healthy cells cultured at 0.5% O(2). Vascular endothelial growth factor (VEGF) receptor inhibition resulted in a decrease in angiogenesis in both healthy hypoxic cells and cells from women with preeclampsia. These findings demonstrate that HUVECs from women with preeclampsia exhibit inherent differences in their angiogenic capacity which are apparent in the absence of placental or maternal factors.
Centre for Reproductive Biology, The Queen's Medical Research Institute, University of Edinburgh, Edinburgh, UK. a.moyes@ucl.ac.uk
Amie J
AJ
Moyes
David
D
Maldonado-Pérez
Gillian A
GA
Gray
Fiona C
FC
Denison
D000328:Adult; D000704:Analysis of Variance; D020533:Angiogenesis Inhibitors / Q000494:pharmacology; D016022:Case-Control Studies; D015687:Cell Hypoxia; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D005260:Female; D006801:Humans; D007211:Indoles / Q000494:pharmacology; D009389:Neovascularization, Pathologic / Q000378:metabolism / Q000503:physiopathology*; D018919:Neovascularization, Physiologic / Q000187:drug effects; D011225:Pre-Eclampsia / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D011247:Pregnancy; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D011758:Pyrroles / Q000494:pharmacology; D040262:Receptors, Vascular Endothelial Growth Factor / Q000037:antagonists & inhibitors / Q000378:metabolism; D014471:Umbilical Veins / Q000187:drug effects / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D055815:Young Adult
D020533:Angiogenesis Inhibitors; D007211:Indoles; D047428:Protein Kinase Inhibitors; D011758:Pyrroles; C116890:Semaxinib; D040262:Receptors, Vascular Endothelial Growth Factor