Immune events associated with high level protection against Schistosoma japonicum infection in pigs immunized with UV-attenuated cercariae.
BACKGROUND
The vaccination of radiation-attenuated Schistosoma japonicum cercariae can induce effective protection in artiodactyl, but the immune events related to protective immunity are not fully understood. To provide a paradigm for a human recombinant antigen vaccine, we have undertaken a vaccination and challenge experiment in pigs, which was recognized as an appropriate animal model in this type of study because of their similarity to human in immunology, and investigated the relative immune events induced by the radiation-attenuated S. japonicum cercariae.


RESULTS
We found that pigs immunized once with 400 µw UV-irradiated cercariae exhibited 63.84% and 71.82% reductions in worm burden and hepatic eggs respectively. Protective immunity in vaccinated pigs was associated with high level productions of IgM, total IgG, IgG1 and IgG2; IgG2 was significantly increased in the acute infection. IFN-γ levels could be elicited by immunization. At week 6 post-infection, IFN-γ, IL-4 and IL-10 levels also showed a dramatic rise synchronously in vaccinated pigs. Moreover, the granzyme b, nk-lysin, ifnγ, il4 and il10 mRNA levels in early skin-draining lymph nodes of immunized pigs were higher than those in pigs with non-irradiated cercariae infection. In addition, cytotoxicity-related genes in the mesenteric lymph nodes were significantly upregulated in vaccinated pigs in the acute infection.


CONCLUSIONS
Our results demonstrated that IFN-γ and IgG2 antibody production, as well as genes related to cytotoxicity are associated with the high level protection induced by UV-irradiated Schistosoma japonicum vaccine. These findings indicated that optimal vaccination against S. japonicum required the induction of IFN-γ, IgG2 antibody related to Th1 responses and cytotoxicity effect.
Tian
Fang
F
Department of Pathogen Biology and Immunology, Nanjing Medical University, Nanjing, Jiangsu, China.
Lin
Dandan
D
Wu
Jingjiao
J
Gao
Yanan
Y
Zhang
Donghui
D
Ji
Minjun
M
Wu
Guanling
G
D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis; D001483:Base Sequence; D058487:Cercaria / Q000276:immunology / Q000528:radiation effects; D016207:Cytokines / Q000097:blood / Q000235:genetics; D017931:DNA Primers; D007114:Immunization; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012549:Schistosoma japonicum / Q000276:immunology*; D012552:Schistosomiasis / Q000469:parasitology / Q000517:prevention & control*; D013552:Swine; D014466:Ultraviolet Rays
D000909:Antibodies, Helminth; D016207:Cytokines; D017931:DNA Primers; D012333:RNA, Messenger