First isolation of Pseudocohnilembus persalinus (Ciliophora: Scuticociliatida) from freshwater-reared rainbow trout, Oncorhynchus mykiss.
Ciliated protists were isolated from the ovarian fluid of apparently healthy adult rainbow trout (Oncorhynchus mykiss) maintained in freshwater. The organism was identified as Pseudocohnilembus persalinus based on morphometric and morphological analysis of silver-stained specimens obtained from culture and on analysis of ribosomal RNA gene sequences. The cytochrome c oxidase subunit 1 gene sequence of this organism also was characterized. This ciliate has been reported previously as free living only in saline environments and as an endosymbiont in a marine teleost, the olive flounder (Paralichthys olivaceus). A cyst-like stage may have facilitated the novel occurrence of this organism as an endosymbiont in rainbow trout.
Jones
Simon R M
SR
Pacific Biological Station, Nanaimo, British Columbia V9T 6N7, Canada. simon.jones@dfo-mpo.gc.ca
Prosperi-Porta
Gina
G
LaPatra
Scott E
SE
D000818:Animals; D016770:Ciliophora Infections / Q000469:parasitology / Q000662:veterinary*; D004272:DNA, Mitochondrial / Q000737:chemistry; D016054:DNA, Protozoan / Q000737:chemistry; D004275:DNA, Ribosomal / Q000737:chemistry; D003576:Electron Transport Complex IV / Q000235:genetics; D005260:Female; D005393:Fish Diseases / Q000469:parasitology*; D005618:Fresh Water; D008969:Molecular Sequence Data; D016802:Oligohymenophorea / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000648:ultrastructure; D017686:Oncorhynchus mykiss / Q000469:parasitology*; D010053:Ovary / Q000469:parasitology; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D016415:Sequence Alignment / Q000662:veterinary; D013559:Symbiosis
D004272:DNA, Mitochondrial; D016054:DNA, Protozoan; D004275:DNA, Ribosomal; D012337:RNA, Ribosomal, 18S; D003576:Electron Transport Complex IV