Cxcl16 interact with SARS-CoV N protein in and out cell.
Our study investigated the host cell protein which can interact with SARS-CoV N protein, and explored the functional connections. The eukaryotic expression vectors pEGFP-N1/SARS-CoVN and pdsRed2-N1/CXCL16 were constructed and used to co-transfect HEK293FT cells by the calcium phosphate method. The HIS-tagged fusion protein SARS-CoVN-GFP was then built and purified for the binding assay in vitro. The co-localization of SARS-CoVN and CXCL16 in the cytoplasm of HEK293FT cells was also shown using confocal laser scanning microscopy. It is suggested that their interaction might be through direct combination. Under a fluorescence microscope, it was observed that the purified fusion protein SARS-CoVN-GFP was attached to the cell membrane of CXCL16-transfected cells, indicating that SARS-CoVN and CXCL16 can be mutually combined.
Zhang
Yuan-Peng
YP
College of Animal Science and Technology, Shandong Agricultural University, Tai'an, 271018, China.
Zhang
Rong-Wu
RW
Chang
Wei-Shan
WS
Wang
Yan-Yan
YY
D002460:Cell Line; D000075743:Chemokine CXCL16; D019743:Chemokines, CXC / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D000086462:Coronavirus Nucleocapsid Proteins; D003593:Cytoplasm / Q000737:chemistry; D015870:Gene Expression; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D018613:Microscopy, Confocal; D019590:Nucleocapsid Proteins / Q000235:genetics / Q000378:metabolism*; D010957:Plasmids; D011485:Protein Binding; D051116:Receptors, Scavenger / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism
C423727:CXCL16 protein, human; D000075743:Chemokine CXCL16; D019743:Chemokines, CXC; D000086462:Coronavirus Nucleocapsid Proteins; D019590:Nucleocapsid Proteins; D051116:Receptors, Scavenger; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins