Surface IgM on DT40 cells may be a component of the putative receptor complex responsible for the binding of infectious bursal disease virus.
To investigate the host-pathogen interactions between infectious bursal disease virus (IBDV) and target B-lymphocytic cells, a cDNA T7 phage display library from the chicken bursa of Fabricius was constructed and screened for virus binding. Surface immunoglobulin M (sIgM) was isolated as a putative candidate binding site and its interactions with IBDV were further investigated using a chicken bursal lymphoma-derived cell line DT40. The results showed that the λ light chain of sIgM specifically interacted with IBDV in a virulence-independent manner in vitro, and most of the binding of IBDV to DT40 cells was inhibited by sIgM-specific monoclonal antibodies. Further, the infectivity of IBDV in vitro was reduced by sIgM-specific monoclonal antibodies. Our data provided evidence that sIgM may participate as one of the putative membrane binding sites responsible for IBDV infection.
Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, China.
Jun
J
Luo
Hong
H
Zhang
Man
M
Teng
Jian-Ming
JM
Fan
Lei-Ming
LM
You
Zhi-Jun
ZJ
Xiao
Ming-Lin
ML
Yi
Yu-Bao
YB
Zhi
Xue-Wu
XW
Li
Gai-Ping
GP
Zhang
D000818:Animals; D045744:Cell Line, Tumor; D002645:Chickens; D005786:Gene Expression Regulation; D007075:Immunoglobulin M / Q000378:metabolism*; D007243:Infectious bursal disease virus / Q000276:immunology*; D008214:Lymphocytes / Q000378:metabolism*; D008565:Membrane Proteins / Q000276:immunology / Q000378:metabolism*; D019151:Peptide Library; D011485:Protein Binding
D007075:Immunoglobulin M; D008565:Membrane Proteins; D019151:Peptide Library