Completion of the full-length genome sequence of human parainfluenza virus types 4A and 4B: sequence analysis of the large protein genes and gene start, intergenic and end sequences.
We have already reported the nucleotide sequences of the NP, P/V, M, F and HN genes of human parainfluenza virus type 4A (hPIV-4A) and type 4B (hPIV-4B). Here, we have determined the sequences of the L protein genes as well as the gene start, intergenic and end sequences, thereby completing the full-length genome sequence of hPIV-4A and 4B. hPIV-4A and 4B have 17,052 and 17,304 nucleotides, respectively. The end sequence of hPIV-4, especially 4B, was extraordinarily long. In a comparison with members of the genus Rubulavirus, the hPIV-4 L proteins were closely related to those of mumps virus (MUV) and hPIV-2, less closely related to those of Menangle virus and Tioman virus, and more distantly related to those of Mapuera virus and porcine rubulavirus.
Komada
Hiroshi
H
Department of Microbiology, Suzuka University of Medical Science Suzuka, Mie, Japan. komada@suzuka-u.ac.jp
Kawano
Mitsuo
M
Uefuji
Atsuo
A
Ito
Morihiro
M
Tsurudome
Masato
M
Hatakeyama
Eri
E
Nakanishi
Mai
M
Sakue
Shiho
S
Joh
Chika
C
Suzumura
Eriko
E
Tamaki
Tatsuya
T
Tomioka
Toshifumi
T
Nishio
Machiko
M
Tsumura
Hideki
H
Uematsu
Jun
J
Yamamoto
Hidetaka
H
O'Brien
Myles
M
Bando
Hisanori
H
Ito
Yasuhiko
Y
D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D018388:Codon, Terminator / Q000235:genetics*; D021901:DNA, Intergenic / Q000235:genetics*; D007668:Kidney / Q000166:cytology; D008969:Molecular Sequence Data; D029482:Parainfluenza Virus 4, Human / Q000145:classification* / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*; D024363:Transcription Initiation Site
D018388:Codon, Terminator; D021901:DNA, Intergenic; D012367:RNA, Viral