Chemical genetics approach to identify new small molecule modulators of cell growth by phenotypic screening of Saccharomyces cerevisiae strains with a library of morpholine-derived compounds.
A chemical genetics approach has been applied in the screening of yeast deletants strains with a pool of morpholine-derived compounds in order to identify candidate small molecules able to produce phenotypic effects on yeast cells. The analysis of the effects of structurally diverse molecules towards cell growth rate in both exponential and stationary phases provides a tool to select candidate compounds for subsequent assays to identify new chemical entities as chemical probes for drug discovery.
Trabocchi
Andrea
A
Department of Chemistry Ugo Schiff, University of Florence, Polo Scientifico e Tecnologico, Via della Lastruccia 13, I-50019, Sesto F.no, Florence, Italy. andrea.trabocchi@unifi.it
Stefanini
Irene
I
Morvillo
Manfredi
M
Ciofi
Leonardo
L
Cavalieri
Duccio
D
Guarna
Antonio
A
D049109:Cell Proliferation / Q000187:drug effects*; D008958:Models, Molecular; D009025:Morpholines / Q000737:chemistry* / Q000494:pharmacology; D010641:Phenotype; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000187:drug effects* / Q000235:genetics; D054852:Small Molecule Libraries
D009025:Morpholines; D054852:Small Molecule Libraries; C037574:morpholine