Relationship between advanced glycation end products and increased lipid peroxidation in semen of diabetic men.
OBJECTIVE
Majority of diabetic male patients have disturbances in their reproductive systems. However, the mechanisms underlying these disturbances are largely unknown. Since advanced glycation end products (AGE) have a key role in oxidative stress and cell damage in diabetic complications, we hypothesize that AGEs may be involved sperm lipid peroxidation.


METHODS
total AGEs in seminal plasma of 32 diabetic and 35 non-diabetic men was determined by spectrofluorimetric method and carboxy methyl lysine (CML) level was assayed using ELISA. Contents of lipid peroxidation in sperm and seminal plasma were determined by thiobarbituric acid reaction. Total antioxidant capacity (TAC) was measured by a colorimetric assay.


RESULTS
total AGEs were found significantly higher in seminal plasma of diabetic men than non-diabetic group (p<0.001) whereas no significant differences in seminal plasma CML values between two groups was observed. Moreover, sperm and seminal plasma lipid peroxidation were significantly higher in diabetic subjects than non-diabetic men and a significantly lower TAC was detected in diabetic group compare to non-diabetics.


CONCLUSIONS
these results showed an increment in AGEs in seminal plasma of diabetic subjects and may suggest a key role for glycation process and increased oxidative stress in reproductive system dysfunction.
Department of Biochemistry and Nutrition, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
J
J
Karimi
M T
MT
Goodarzi
H
H
Tavilani
I
I
Khodadadi
I
I
Amiri
D000328:Adult; D000975:Antioxidants; D001786:Blood Glucose / Q000032:analysis; D048909:Diabetes Complications / Q000378:metabolism; D003920:Diabetes Mellitus / Q000097:blood / Q000378:metabolism* / Q000503:physiopathology; D006442:Glycated Hemoglobin / Q000032:analysis; D017127:Glycation End Products, Advanced / Q000378:metabolism*; D006801:Humans; D007248:Infertility, Male / Q000378:metabolism; D015227:Lipid Peroxidation; D008239:Lysine / Q000031:analogs & derivatives / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008875:Middle Aged; D018384:Oxidative Stress; D012661:Semen / Q000378:metabolism*; D013094:Spermatozoa / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D055815:Young Adult
D000975:Antioxidants; D001786:Blood Glucose; D006442:Glycated Hemoglobin; D017127:Glycation End Products, Advanced; D008239:Lysine; D008315:Malondialdehyde; D017392:Thiobarbituric Acid Reactive Substances; C517652:hemoglobin A1c protein, human; C048496:N(6)-carboxymethyllysine