Iopamidol as a responsive MRI-chemical exchange saturation transfer contrast agent for pH mapping of kidneys: In vivo studies in mice at 7 T.
Iopamidol (Isovue®-Bracco Diagnostic Inc.) is a clinically approved X-Ray contrast agent used in the last 30 years for a wide variety of diagnostic applications with a very good clinical acceptance. Iopamidol contains two types of amide functionalities that can be exploited for the generation of chemical exchange saturation transfer effect. The exchange rate of the two amide proton pools is markedly pH-dependent. Thus, a ratiometric method for pH assessment has been set-up based on the comparison of the saturation transfer effects induced by selective irradiation of the two resonances. This ratiometric approach allows to rule out the concentration effect of the contrast agent and provides accurate pH measurements in the 5.5-7.4 range. Upon injection of Iopamidol into healthy mice, it has been possible to acquire pH maps of kidney regions. Furthermore, it has been also shown that the proposed method is able to report about pH-changes induced in control mice fed with acidified or basified water for a period of a week before image acquisition.
Department of Chemistry IFM and Center for Molecular Imaging, University of Torino, Torino, Italy.
Dario Livio
DL
Longo
Walter
W
Dastrù
Giuseppe
G
Digilio
Jochen
J
Keupp
Sander
S
Langereis
Stefania
S
Lanzardo
Simone
S
Prestigio
Oliver
O
Steinbach
Enzo
E
Terreno
Fulvio
F
Uggeri
Silvio
S
Aime
D000818:Animals; D003287:Contrast Media / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007479:Iopamidol / Q000737:chemistry*; D007668:Kidney / Q000737:chemistry*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D051379:Mice; D008807:Mice, Inbred BALB C
D003287:Contrast Media; D007479:Iopamidol