Identification strategy using combined mass spectrometric techniques for elucidation of phase I and phase II in vitro metabolites of lipophilic marine biotoxins.
Combining mass spectrometric tools, a total of 47 in vitro metabolites of okadaic acid (OA), dinophysistoxins 1 and 2 (DTX1 and DTX2), yessotoxin (YTX), azaspiracid1 (AZA1), and pectenotoxin 2 (PTX2) could be detected and confirmed after an incubation with rat liver S9-mix. In a first step, liquid chromatography (LC) combined with tandem mass spectrometry (MS/MS) was used as a screening tool for the identification of in vitro metabolites of lipophilic marine biotoxins. Metabolic phase I and phase II reactions were screened for metabolites by calculating and subsequently monitoring theoretical MS transitions. In a second step, metabolites were confirmed by determination of accurate masses using high resolution MS provided by Orbitrap technology. Subsequently, product ion spectra, precursor ion spectra, and MS3 spectra were recorded for structure elucidation of metabolites. While all investigated toxins were found to form various oxygenated metabolites during the oxidative phase I metabolism, those metabolites varied in the number of added oxygen atoms and in the number of individual isomers. No hints were obtained concerning the formation of glutathione adducts, and a conjugation with glucuronic acid was detected for AZA1 only.
National Reference Laboratory for Marine Biotoxins, Federal Institute for Risk Assessment, Thielallee 88-92, 14195 Berlin, Germany.
Katrin
K
Kittler
Angelika
A
Preiss-Weigert
Anja
A
These
D000818:Animals; D005663:Furans / Q000378:metabolism; D057927:Hydrophobic and Hydrophilic Interactions; D008099:Liver / Q000201:enzymology / Q000378:metabolism; D018942:Macrolides; D008297:Male; D008387:Marine Toxins / Q000737:chemistry* / Q000378:metabolism*; D050216:Metabolic Detoxication, Phase I; D050217:Metabolic Detoxication, Phase II; D008978:Mollusk Venoms; D035964:Oxocins / Q000378:metabolism; D011714:Pyrans / Q000378:metabolism; D051381:Rats; D057096:Shellfish Poisoning / Q000378:metabolism; D013141:Spiro Compounds / Q000378:metabolism; D053719:Tandem Mass Spectrometry / Q000379:methods*; D000095702:Polyether Toxins
D005663:Furans; D018942:Macrolides; D008387:Marine Toxins; D008978:Mollusk Venoms; D035964:Oxocins; D011714:Pyrans; D013141:Spiro Compounds; C406592:azaspiracid; D000095702:Polyether Toxins; C098380:pectenotoxin 2; C066632:yessotoxin