Multiple biological complex of alkaline extract of the leaves of Sasa senanensis Rehder.
Previous studies have shown anti-inflammatory potential of alkaline extract of the leaves of Sasa senanensis Rehder (SE). The aim of the present study was to clarity the molecular entity of SE, using various fractionation methods. SE inhibited the production of nitric oxide (NO), but not tumour necrosis factor-α by lipopolysaccharide (LPS)-stimulated mouse macrophage-like cells. Lignin carbohydrate complex prepared from SE inhibited the NO production to a comparable extent with SE, whereas chlorophyllin was more active. On successive extraction with organic solvents, nearly 90% of SE components, including chlorophyllin, were recovered from the aqueous layer. Anti-HIV activity of SE was comparable with that of lignin-carbohydrate complex, and much higher than that of chlorophyllin and n-butanol extract fractions. The CYP3A inhibitory activity of SE was significantly lower than that of grapefruit juice and chlorophyllin. Oral administration of SE slightly reduced the number of oral bacteria. When SE was applied to HPLC, nearly 70% of SE components were eluted as a single peak. These data suggest that multiple components of SE may be associated with each other in the native state or after extraction with alkaline solution.
Division of Pharmacology, Department of Diagnostic and Therapeutic Sciences, Meikai University School of Dentistry, Sakado, Saitama 350-0283, Japan. sakagami@dent.meikai.ac.jp
Hiroshi
H
Sakagami
Li
L
Zhou
Michiyo
M
Kawano
May Maw
MM
Thet
Shoji
S
Tanaka
Mamoru
M
Machino
Shigeru
S
Amano
Reina
R
Kuroshita
Shigeru
S
Watanabe
Qing
Q
Chu
Qin-Tao
QT
Wang
Taisei
T
Kanamoto
Shigemi
S
Terakubo
Hideki
H
Nakashima
Keisuke
K
Sekine
Yoshiaki
Y
Shirataki
Chang-Hao
CH
Zhang
Yoshihiro
Y
Uesawa
Kiminori
K
Mohri
Madoka
M
Kitajima
Hiroshi
H
Oizumi
Takaaki
T
Oizumi
D000468:Alkalies / Q000008:administration & dosage*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage*; D016866:Bacteroidaceae Infections / Q000188:drug therapy / Q000276:immunology / Q000382:microbiology; D002460:Cell Line; D002735:Chlorophyllides / Q000494:pharmacology; D032083:Citrus paradisi / Q000737:chemistry; D015658:HIV Infections / Q000188:drug therapy; D006801:Humans; D008031:Lignin / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051379:Mice; D008862:Microsomes, Liver / Q000187:drug effects; D009569:Nitric Oxide / Q000378:metabolism; D010936:Plant Extracts / Q000008:administration & dosage*; D016966:Porphyromonas gingivalis / Q000187:drug effects / Q000254:growth & development; D051381:Rats; D031787:Sasa / Q000737:chemistry*; D013280:Stomatitis / Q000188:drug therapy* / Q000276:immunology / Q000382:microbiology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D000468:Alkalies; D000893:Anti-Inflammatory Agents; D002735:Chlorophyllides; D008070:Lipopolysaccharides; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; D008031:Lignin; C007020:chlorophyllin