LIM domain protein FHL1B interacts with PP2A catalytic β subunit--a novel cell cycle regulatory pathway.
Four-and-a-half LIM domain protein 1B (FHL1B) is an alternatively-spliced isoform of FHL1. In this study, FHL1B was demonstrated to interact with the β catalytic subunit (Cβ) of a type 2A protein phosphatase (PP2A) by yeast two-hybrid screening. Domain studies using a small-scale yeast two-hybrid interaction assay revealed the mediation of protein-protein interaction by FHL1B's C-terminus. Interaction between FHL1B and PP2A was further verified by co-immunoprecipitation. FHL1B was also shown to shuttle between nucleus and cytoplasm at different phases of the cell cycle. These data suggest that the FHL1B/PP2A(Cβ) interaction may illustrate a novel cell-cycle regulatory pathway.
School of Biomedical Sciences, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong.
Chi-Hang
CH
Wong
Yin-Wan Wendy
YW
Fung
Enders Kai-On
EK
Ng
Simon Ming-Yuen
SM
Lee
Mary Miu-Yee
MM
Waye
Stephen Kwok-Wing
SK
Tsui
D000818:Animals; D055162:Biocatalysis; D020134:Catalytic Domain; D002453:Cell Cycle; D045744:Cell Line, Tumor; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D042541:Intracellular Space / Q000378:metabolism; D060588:LIM Domain Proteins; D051379:Mice; D009124:Muscle Proteins / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D054648:Protein Phosphatase 2 / Q000737:chemistry* / Q000378:metabolism*; D021381:Protein Transport; D015203:Reproducibility of Results; D017384:Sequence Deletion; D013379:Substrate Specificity
C101149:FHL1 protein, human; D047908:Intracellular Signaling Peptides and Proteins; D060588:LIM Domain Proteins; D009124:Muscle Proteins; D054648:Protein Phosphatase 2