In vitro analysis of an rhGDF-5 suture coating process and the effects of rhGDF-5 on rat tendon fibroblasts.
We describe a dipcoating method of coating 4-0 VICRYL sutures with recombinant human growth and differentiation factor-5 (rhGDF-5), and analyze the in vitro effects of rhGDF-5 on rat tendon fibroblasts (RTFs). Part I: Eight 4-0 VICRYL sutures were coated in a dipcoat solution using rhGDF-5 solutions at concentrations of 0, 40, 200, and 1000 μg/ml (n=32). ELISA was performed to determine the amount of rhGDF-5 that was transferred on the suture. Part II: Using a dipcoat solution of 200 μg/ml, four sutures were passed through rat tendon, and quantified ELISA was again performed. Cell proliferation, collagen synthesis, and RTF cell migration were also analyzed. The differences in the amount rhGDF-5 transferred on the suture between the different concentration groups were statistically significant. Furthermore, rhGDF-5-stimulated RTF cell migration, cell proliferation, and collagen synthesis at dipcoat concentrations of rhGDF-5 of at least 200 μg/ml.
Hospital for Special Surgery, New York, NY 10021, USA.
Joshua S
JS
Dines
Michael B
MB
Cross
David
D
Dines
Christina
C
Pantazopoulos
Han Jo
HJ
Kim
Pasquale
P
Razzano
Daniel
D
Grande
D000818:Animals; D002465:Cell Movement; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000494:pharmacology*; D003094:Collagen / Q000378:metabolism; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D055428:Growth Differentiation Factor 5 / Q000008:administration & dosage / Q000494:pharmacology*; D006801:Humans; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000494:pharmacology*; D013537:Sutures; D013710:Tendons / Q000166:cytology / Q000187:drug effects*
D020099:Coated Materials, Biocompatible; C525893:GDF5 protein, human; D055428:Growth Differentiation Factor 5; D011994:Recombinant Proteins; D003094:Collagen