Monolysocardiolipin: improved preparation with high yield.
A simple, high-yielding preparation of monolysocardiolipin (MLCL) by phospholipase A2 hydrolysis of cardiolipin (CL) in methanol on a semi-preparative scale is described. In methanol, phospholipase A2 preferentially hydrolyzes CL to MLCL. This selectivity results in ∼80% yield of MLCL. The synthesized MLCL and dilysocardiolipin were characterized by NMR and ESI-MS/MS. Only the sn-2 position of CL was hydrolyzed by phospholipase A2 in methanol.
Department of Pharmacology, Case Western Reserve University, Cleveland, OH 44106, USA.
Junhwan
J
Kim
Charles L
CL
Hoppel
D000818:Animals; D002308:Cardiolipins / Q000302:isolation & purification* / Q000378:metabolism; D002417:Cattle; D008246:Lysophospholipids / Q000302:isolation & purification*; D019906:Nuclear Magnetic Resonance, Biomolecular; D054467:Phospholipases A2 / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
D002308:Cardiolipins; D008246:Lysophospholipids; C067188:monolysocardiolipin; D054467:Phospholipases A2