Regulation of acid phosphatase in reverse micellar system by lipids additives: structural aspects.
Reverse micelles system is suggested as a direct tool to study the influence of membrane matrix composition on the activity and structure of membrane-associated enzymes with the use of acid phosphatase (AP) as an example. In reverse micelles the functioning of the monomeric and dimeric forms of AP could be separately observed by variation of the size of the micelles. We found that including the lipids into the micellar system can dramatically affect the enzyme functioning even at low lipid content (2% w/w), and this effect depends on the lipid nature. Structural studies using CD spectroscopy and DLS methods have shown that the influence of lipid composition on the enzyme properties might be caused by the interaction of lipids with the enzyme as well as by the influence of lipids on structure and properties of the micellar matrix.
Department of Chemical Enzymology, Chemical Faculty, Lomonosov Moscow State University, 119991 Moscow, Russia. helena_koudriachova@hotmail.com
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Kudryashova
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Bronza
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Vinogradov
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A
Kamyshny
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Magdassi
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Levashov
D000135:Acid Phosphatase / Q000737:chemistry / Q000378:metabolism*; D002942:Circular Dichroism; D004143:Dioctyl Sulfosuccinic Acid / Q000737:chemistry / Q000378:metabolism; D050356:Lipid Metabolism; D008055:Lipids / Q000737:chemistry*; D008567:Membranes, Artificial; D008823:Micelles; D011487:Protein Conformation; D055503:Protein Multimerization; D013501:Surface-Active Agents / Q000737:chemistry / Q000378:metabolism; D014908:Triticum / Q000201:enzymology*
D008055:Lipids; D008567:Membranes, Artificial; D008823:Micelles; D013501:Surface-Active Agents; D004143:Dioctyl Sulfosuccinic Acid; D000135:Acid Phosphatase