Immunohistochemical localization of D-aspartate oxidase in porcine peripheral tissues.
D-Aspartate (D-Asp) is an endogenous substance in mammals. Degradation of D-Asp is carried out only by D-aspartate oxidase (DDO). We measured DDO activity in porcine tissues, and produced an anti-porcine DDO antibody to examine the cellular localization of DDO. All the tissues examined showed DDO activities, whereas the substrate D-Asp was not detected in kidney cortex, liver, heart, and gastric mucosa. In the kidney, intensive immunohistochemical staining for DDO was found in the epithelial cells of the proximal tubules. In the liver, the epithelial cells of interlobular bile ducts, liver sinusoid-lining cells with cytoplasmic processes, and the smooth muscle cells of arterioles were strongly stained for DDO. In the heart, cardiomyocytes and the smooth muscle cells of arterioles showed DDO-immunoreactivity. In the gastric mucosa, only the chief cells were DDO-positive. These newly identified DDO-positive cells seem to actively degrade D-Asp to prevent an excess of D-Asp from exerting harmful effects on the respective functions of porcine tissues.
Department of Biochemistry and Molecular Biology, Shiga University of Medical Science, Seta, Ohtsu, Shiga, 520-2192, Japan.
Atsushi
A
Yamamoto
Hiroyuki
H
Tanaka
Tetsuo
T
Ishida
Kihachiro
K
Horiike
D000818:Animals; D001160:Arterioles / Q000033:anatomy & histology / Q000378:metabolism; D003331:Coronary Vessels / Q000033:anatomy & histology / Q000378:metabolism; D050804:D-Aspartate Oxidase / Q000378:metabolism*; D057075:Enzyme Assays; D005260:Female; D005753:Gastric Mucosa / Q000033:anatomy & histology / Q000378:metabolism; D006325:Heart Atria / Q000033:anatomy & histology / Q000378:metabolism; D007106:Immune Sera / Q000302:isolation & purification; D007672:Kidney Cortex / Q000033:anatomy & histology / Q000378:metabolism*; D007686:Kidney Tubules, Distal / Q000033:anatomy & histology / Q000378:metabolism; D007687:Kidney Tubules, Proximal / Q000033:anatomy & histology / Q000378:metabolism; D008099:Liver / Q000033:anatomy & histology / Q000378:metabolism; D009928:Organ Specificity; D011817:Rabbits; D013552:Swine
D007106:Immune Sera; D050804:D-Aspartate Oxidase