[Suppression subtractive hybridization for the identification of differentially expressed genes in the hippocampus of the offsprings of lead exposed female rats].
OBJECTIVE
To screen and identify differentially expressed genes in the hippocampus of the offsprings of lead exposed female rats in order to provide a theoretical basis for identifying learning and memory deficits related genes.


METHODS
RNA was extracted from the hippocampus of young rats with learning and memory deficits due to maternal lead exposure. Suppression subtractive hybridization was used to identify the differentially expressed genes in the hippocampus.


RESULTS
An effective subtracted library was constructed which consisted of approximately 200 clones. Sequencing for the library identified 93 clones harboring insertion fragments which included 43 different genes and 4 unknown genes. These genes might be related to learning and memory deficits due to maternal lead exposure.


CONCLUSIONS
The up-regulated genes in the hippocampus of young rats from pregnant rats under lead exposure include some housekeeping genes and some proteins involved in cellular protein folding, signal transduction, stress response and DNA methylation. These proteins might be directly related to a significant reduction in learning and memory abilities in the young rats.
Department of Child Health Care, Children's Hospital of Jiangxi Province, Nanchang, China.
Shou-Ming
SM
Li
Chang-Ying
CY
Liu
Guo-Xiu
GX
Xie
Ming
M
Zhou
Zhi-Yong
ZY
Liu
D000818:Animals; D005260:Female; D020869:Gene Expression Profiling; D015723:Gene Library; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism; D007854:Lead / Q000633:toxicity*; D007858:Learning / Q000187:drug effects; D008568:Memory / Q000187:drug effects; D009693:Nucleic Acid Hybridization / Q000379:methods*; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D051381:Rats
D007854:Lead