Loss of carotene-9',10'-monooxygenase expression increases serum and tissue lycopene concentrations in lycopene-fed mice.
Two enzymes have been identified for the oxidative metabolism of carotenoids in mammals. Carotene-15,15'-monooxygenase (CMO-I) primarily centrally cleaves β,β-carotene to form vitamin A. We hypothesize that carotene-9',10'-monooxygenase (CMO-II) plays a key role in metabolism of acyclic nonprovitamin A carotenoids such as lycopene. We investigated carotenoid bioaccumulation in young adult, male, wild-type (WT) mice or mice lacking CMO-II (CMO-II KO). Mice were fed an AIN-93G diet or identical diets supplemented with 10% tomato powder, 130 mg lycopene/kg diet (10% lycopene beadlets), or placebo beadlets for 4 or 30 d. Lycopene preferentially accumulated in CMO-II KO mouse tissues and serum compared with WT mouse tissues. β-Carotene preferentially accumulated in some CMO-II KO mouse tissues compared with WT mouse tissues. Relative tissue mRNA expression of CMO-I and CMO-II was differentially expressed in mouse tissues, and CMO-II, but not CMO-I, was expressed in mouse prostate. In conclusion, the loss of CMO-II expression leads to increased serum and tissue concentrations of lycopene in tomato-fed mice.
Ford
Nikki A
NA
Division of Nutritional Sciences, University of Illinois, Urbana, IL 61801, USA.
Clinton
Steven K
SK
von Lintig
Johannes
J
Wyss
Adrian
A
Erdman
John W
JW
D000818:Animals; D001835:Body Weight; D002338:Carotenoids / Q000008:administration & dosage / Q000097:blood / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D017931:DNA Primers; D044943:Fatty Acid Desaturases / Q000235:genetics / Q000378:metabolism*; D000077276:Lycopene; D051379:Mice; D008810:Mice, Inbred C57BL; D009929:Organ Size; D010919:Placebos; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics
D017931:DNA Primers; D010919:Placebos; D012333:RNA, Messenger; D002338:Carotenoids; D044943:Fatty Acid Desaturases; C554481:carotene-9',10'-monooxygenase, mouse; D000077276:Lycopene