The small ribosomal protein S12P gene rpsL as an efficient positive selection marker in allelic exchange mutation systems for Corynebacterium glutamicum.
We report that the mutant rpsL K43R in streptomycin-resistant and lysine-producing Corynebacterium glutamicum is responsible for streptomycin resistance. In addition, we describe its effective application in gene modification in C. glutamicum.
Kim
Il Kwon
IK
Laboratory of Immunobiology, School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University, Daegu, South Korea.
Jeong
Weol Kyu
WK
Lim
Seong Han
SH
Hwang
In Kwan
IK
Kim
Young Ho
YH
D019943:Amino Acid Substitution / Q000235:genetics; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D048230:Corynebacterium glutamicum / Q000187:drug effects / Q000235:genetics*; D024881:Drug Resistance, Bacterial; D018390:Gene Targeting; D005827:Genetics, Microbial / Q000379:methods*; D020125:Mutation, Missense; D011995:Recombination, Genetic; D012269:Ribosomal Proteins / Q000235:genetics*; D012641:Selection, Genetic; D013307:Streptomycin / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D012269:Ribosomal Proteins; C034212:ribosomal protein S12; D013307:Streptomycin