[Microbial lipid production by Rhodosporidium toruloides in a two-stage culture mode].
To shorten the cultivation time and reduce the consumption of raw materials for microbial lipid production, oleaginous yeast Rhodosporidium toruloides AS 2.1389 was cultivated using a two-stage culture mode, in which the cell propagation and lipid accumulation were separated. The yeast cells recovered from the propagation culture were re-suspended in glucose solution for lipid accumulation, through which lipid content over 55% of the dry cell weight was achieved, the longer the propagation stage was, the higher the lipid content. Analysis of the lipid indicated that the long-chain fatty acids with 16 and 18 carbon atoms were major components, suggesting that the lipid can be an alternative feedstock for biodiesel production.
Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
Jintao
J
Lin
Hongwei
H
Shen
Zehui
Z
Zhang
Cuimin
C
Hu
Guojie
G
Jin
Haidong
H
Tan
Zongbao K
ZK
Zhao
D001487:Basidiomycota / Q000254:growth & development* / Q000378:metabolism*; D056804:Biofuels; D018929:Cell Culture Techniques / Q000379:methods*; D005285:Fermentation; D007218:Industrial Microbiology / Q000379:methods*; D008055:Lipids / Q000096:biosynthesis*
D056804:Biofuels; D008055:Lipids