Effects of surface pre-reacted glass-ionomer fillers on mineral induction by phosphoprotein.
OBJECTIVE
The aim of this study was to evaluate the mineralizing potential of ions released from surface pre-reacted glass-ionomer (S-PRG) fillers on mineral induction by phosphoprotein in vitro.


METHODS
Phosvitin was used as a model of dentin phosphoprotein in this study. Phosvitin was immobilized on agarose beads with divinyl sulfone. Five aliquots of phosvitin-immobilized agarose beads were incubated in control or experimental mineralizing solution. The experimental mineralizing solutions were made from eluates of resin filled with S-PRG fillers. The beads were incubated at 37°C in a shaking water bath, and aliquots were taken at several time points during the incubation. Then the beads were analyzed for calcium by atomic absorption spectrometry.


RESULTS
Phosvitin-immobilized agarose beads induced mineral formation after incubation for 5.3h in the metastable solution without ions eluted from S-PRG fillers. Undiluted eluates significantly reduced mineral induction time. SEM observation and X-ray diffraction revealed larger apatite crystals on the beads incubated with eluates of S-PRG fillers than with the control.


CONCLUSIONS
S-PRG fillers may play a role in mineral induction.
Division of Clinical Cariology and Endodontology, Department of Oral Rehabilitation, School of Dentistry, Health Sciences University of Hokkaido, 1757 Tobetsu, Hokkaido 0610293, Japan. shu@hoku-iryo-u.ac.jp
Shuichi
S
Ito
Masahiro
M
Iijima
Masanori
M
Hashimoto
Naohiro
N
Tsukamoto
Itaru
I
Mizoguchi
Takashi
T
Saito
D000180:Acrylic Resins / Q000737:chemistry*; D001031:Apatites / Q000032:analysis / Q000737:chemistry*; D017438:Bisphenol A-Glycidyl Methacrylate / Q000737:chemistry; D002118:Calcium / Q000032:analysis; D003188:Composite Resins / Q000737:chemistry*; D003460:Crystallization; D006801:Humans; D055767:Immobilized Proteins / Q000737:chemistry; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D008903:Minerals / Q000737:chemistry*; D010750:Phosphoproteins / Q000737:chemistry*; D010774:Phosvitin / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D011109:Polymethacrylic Acids / Q000737:chemistry; D011140:Polyurethanes / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry*; D013054:Spectrophotometry, Atomic; D013059:Spectrum Analysis, Raman; D013499:Surface Properties; D013696:Temperature; D013997:Time Factors; D014867:Water / Q000737:chemistry; D014961:X-Ray Diffraction
D000180:Acrylic Resins; D001031:Apatites; D003188:Composite Resins; D055767:Immobilized Proteins; D008903:Minerals; D010750:Phosphoproteins; D011109:Polymethacrylic Acids; D011140:Polyurethanes; C015897:glass ionomer; C050845:urethane acrylate; D014867:Water; C020946:triethylene glycol dimethacrylate; D011092:Polyethylene Glycols; D017438:Bisphenol A-Glycidyl Methacrylate; D012822:Silicon Dioxide; D010774:Phosvitin; D002118:Calcium