Dispersion-based pulse shaping for multiplexed two-photon fluorescence microscopy.
We demonstrate selective two-photon excited fluorescence microscopy with shaped pulses produced with a simple yet efficient scheme based on dispersive optical components. The pulse train from a broadband oscillator is split into two subtrains that are sent through different amounts of glass. Beam recombination results in pulse-shape switching at a rate of 150MHz. Time-resolved photon counting detection then provides two simultaneous images resulting from selective two-photon excitation, as demonstrated in a live embryo. Although less versatile than programmable pulse-shaping devices, this novel arrangement significantly improves the performance of selective microscopy using broadband shaped pulses while simplifying the experimental setup.
Labroille
Guillaume
G
Laboratoire d’Optique et Biosciences, Ecole Polytechnique, CNRS, and INSERM U696, 91128 Palaiseau, France.
Pillai
Rajesh S
RS
Solinas
Xavier
X
Boudoux
Caroline
C
Olivier
Nicolas
N
Beaurepaire
Emmanuel
E
Joffre
Manuel
M