Improving the remaining activity of lignocellulolytic enzymes by membrane entrapment.
The production of bioethanol by the conversion of lignocellulosic waste has attracted much interest in recent years because of its low cost and great potential availability. However, the high cost of the enzyme required for this conversion is often considered to be the major bottleneck in the commercial lignocellulosic ethanol industry. In this work, the hydrolysis of rice straw by free and entrapped lignocellulolytic enzymes (cellulase, xylanase and laccase) was carried out at pH 5.5 and 37°C. The hydrolysis of rice straw by enzymes entrapped in a membrane produced a higher monosaccharide content: 601.05 mg/g rice straw for entrapped enzymes vs. 465.46 mg/g rice straw for free enzymes. This study has shown that enzyme entrapment is an important technique for the efficient use and reuse of enzymes in industrial applications and also for the rapid separation of saccharide products from the reaction medium, thus improving the remaining enzymatic activities.
Chang
Ken-Lin
KL
Institute of Biological Chemistry & Genomic Research Center, Academia Sinica, Nankang, Taipei 115, Taiwan.
Thitikorn-amorn
Jitladda
J
Chen
Shan-He
SH
Hsieh
Jung-Feng
JF
Ratanakhanokchai
Khanok
K
Huang
Po-Jung
PJ
Lin
Ting-Chia
TC
Chen
Shui-Tein
ST
D001709:Biotechnology / Q000379:methods*; D002480:Cellulase / Q000378:metabolism*; D043364:Endo-1,4-beta Xylanases / Q000378:metabolism; D004800:Enzymes, Immobilized / Q000378:metabolism*; D005947:Glucose / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D006868:Hydrolysis / Q000187:drug effects; D042845:Laccase / Q000378:metabolism; D008031:Lignin / Q000378:metabolism*; D008567:Membranes, Artificial; D009005:Monosaccharides / Q000032:analysis; D012275:Oryza / Q000187:drug effects / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects; D013696:Temperature
D004800:Enzymes, Immobilized; D008567:Membranes, Artificial; D009005:Monosaccharides; C036909:lignocellulose; D008031:Lignin; D042845:Laccase; D002480:Cellulase; D043364:Endo-1,4-beta Xylanases; D005947:Glucose