The effect of cysteine and glutathione on sperm and oxidative stress parameters of post-thawed bull semen.
The aim of this study was to determine the effects of antioxidants such as reduced glutathione (GSH) and cysteine in Laiciphose® extender on semen parameters, fertilizing ability, lipid peroxidation (LPO) level and glutathione peroxidise (GPx) activity of post-thawed bull semen. Totally 54 ejaculates of three bulls were used in the study. Five groups, namely; GSH (0.5 and 2 mM), cysteine (5 and 10 mM) and control group, were conducted to test the antioxidants in Laiciphose®. Insemination doses were processed that each 0.25-mL straw contained 15 x 10⁶ sperm. The addition of antioxidants did not present any significant effect on the percentages of post-thaw sperm morphology (acrosome and total abnormalities), subjective, CASA and progressive motilities, as well as sperm motility characteristics (VAP, VSL, VCL, LIN and ALH), compared to the control groups (P > 0.05). GSH 0.5mM (55.5±7.38%) and cysteine 10 mM (48±5.65%) led to lower rates of DNA damage, compared to control (P < 0.05). As regards to MDA level, cysteine at 10 mM dose gave the highest level (4.99±0.44 nmol/L) (P < 0.001). GPx activity was demonstrated to be higher level upon the addition of 5 mM cysteine when compared to the other groups (P < 0.05). With respect to fertility results based on 60-day non-returns, the supplementation of antioxidants did not present significant differences (P > 0.05). The results of this study may provide an useful information for the future studies in this area. So, further studies could be suggested to achieve better information in terms of the DNA damage and fertilizing capacity of bull sperm frozen with effective antioxidants.
Tuncer
Pürhan Barbaros
PB
Tarım ve Köyişleri Bakanliği, Lalahan Hayvancilik Merkez Araştirma Enstitüsü, Lalahan, Ankara, Turkey.
Bucak
Mustafa Numan
MN
Büyükleblebici
Serhat
S
Sarıözkan
Serpil
S
Yeni
Deniz
D
Eken
Ayşe
A
Akalın
Pınar Peker
PP
Kinet
Hüseyin
H
Avdatek
Fatih
F
Fidan
A Fatih
AF
Gündoğan
Mustafa
M
D000818:Animals; D002417:Cattle; D003545:Cysteine / Q000494:pharmacology*; D005978:Glutathione / Q000494:pharmacology*; D005979:Glutathione Peroxidase / Q000378:metabolism; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D012662:Semen Preservation / Q000379:methods; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects* / Q000378:metabolism
D005979:Glutathione Peroxidase; D005978:Glutathione; D003545:Cysteine