Expression, purification, and characterization of an iron chaperon protein CyaY from Acidithiobacillus ferrooxidans.
CyaY is the bacterial homolog of frataxin, proposed to be involved in the assembly of iron-sulfur clusters. While, the physiological iron donor for the iron-sulfur clusters assembly remains controversial. In this study, the gene of CyaY from Acidithiobacillus ferrooxidans was cloned and expressed in Escherichia coli, the protein was purified by one-step affinity chromatography to homogeneity. The CyaY protein can bind ferric iron and serve as an iron donor for the biogenesis of iron-sulfur clusters on the scaffold protein IscU in the presence of IscS and L-cysteine in vitro.
Ai
Chenbing
C
Department of Bioengineering, School of Resources Processing and Bioengineering, Central South University, Changsha, 410083, Hunan, People's Republic of China.
Mo
Hongyu
H
Chen
Qian
Q
Liu
Yuandong
Y
Tang
Lin
L
Du
Juan
J
Zeng
Jia
J
D042763:Acidithiobacillus / Q000201:enzymology* / Q000235:genetics; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D002846:Chromatography, Affinity; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005290:Ferric Compounds / Q000378:metabolism; D007501:Iron / Q000378:metabolism; D008958:Models, Molecular; D018832:Molecular Chaperones / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D008969:Molecular Sequence Data; D011485:Protein Binding; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
D001426:Bacterial Proteins; D005290:Ferric Compounds; D018832:Molecular Chaperones; D011994:Recombinant Proteins; D007501:Iron