Fetal calf serum enhances in vitro production of Bos taurus indicus embryos.
The objective of this study was to determine the effect of fetal calf serum (FCS) on the quality of in vitro produced bovine embryos. Cumulus oocyte-complexes (COCs, n = 2 449) recovered by ovum pick-up from Bos taurus indicus donors were randomly assigned to experimental groups. Sperm selected by Percoll gradient was used for in vitro fertilization (insemination = Day 0). In Experiment 1 (n = 1 745 COCs), zygotes were cultured in vitro in Synthetic Oviduct Fluid + 4 mg/mL of bovine serum albumin (BSA), or BSA + 2% FCS (BSA+FCS). In Experiment 2 (n = 704 COCs), the COCs were cultured in SOF + BSA, BSA + 2% FCS, or BSA + 2% FCS on D4 (BSA + FCSD4). In Experiment 1, blastocyst yield (51%) and Quality I blastocysts (41%) at Day 7 were higher (P < 0.05) in the BSA + FCS treatment than in BSA (42 and 30%, respectively). In Experiment 2, blastocyst yield was higher (P < 0.05) in the BSA+FCS (47%) treatment. Quality I blastocyst yield was higher (P < 0.05) for BSA + FCS (34%) and BSA+FCSD4 (32%) compared to the BSA treatment (20%). A total of 820 embryos were transferred, with no significant differences among groups in pregnancy rates. In conclusion, in vitro culture in SOFaaci + BSA + FCS enhanced blastocyst yield and Quality I blastocysts; adding FCS to the culture medium increased the efficiency of IVP of bovine embryos.
Leivas
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FG
Embryolab-Laboratório de Embriologia Animal, Departamento de Clínica de Grandes Animais, Centro de Ciências Rurais, Universidade Federal Santa Maria, 97.105-900 Santa Maria RS, Brazil.
Brum
D S
DS
Fialho
S S
SS
Saliba
W P
WP
Alvim
M T T
MT
Bernardi
M L
ML
Rubin
M I B
MI
Silva
C A M
CA
D000818:Animals; D001755:Blastocyst / Q000502:physiology; D002417:Cattle / Q000196:embryology*; D002478:Cells, Cultured; D003470:Culture Media; D054885:Cumulus Cells; D046149:Embryo Culture Techniques / Q000662:veterinary; D004624:Embryo Transfer / Q000662:veterinary; D047108:Embryonic Development; D005260:Female; D005307:Fertilization in Vitro / Q000662:veterinary*; D005312:Fetal Blood / Q000502:physiology*; D008297:Male; D009865:Oocytes; D011247:Pregnancy; D012710:Serum Albumin, Bovine / Q000008:administration & dosage
D003470:Culture Media; D012710:Serum Albumin, Bovine