Identification of a Passiflora alata Curtis dimeric peptide showing identity with 2S albumins.
Antifungal proteins and peptides, essential compounds for plant defense, have been isolated from several tissues of various plants. These proteins could be used as a natural alternative to control phytopathogenic fungi. In this report a heterodimeric antifungal protein named Pa-AFP1, showing higher identity with the 2S albumin family, was purified by using 70-100% ammonium sulfate saturation and further purification steps such as anionic exchange Q-Sepharose chromatography associated with HPLC reversed-phase C4 chromatography. Analysis by Tricine-SDS-PAGE revealed two peptidic molecular masses of approximately 4500 Da and 7000 Da, in the presence of β-mercaptoethanol, while by removing the reducing agent a single protein with molecular mass of about 11,500 Da was obtained. Moreover, dimer mass was confirmed by MALDI-TOF analyses (11,569.76 Da). The antifungal protein, named Pa-AFP1, efficiently inhibited the growth of filamentous fungi Colletotrichum gloeosporioides, and was added to a short list of 2S albumins with antimicrobial properties. Otherwise, this same peptide showed no activity toward bacteria and yeasts. In summary, this compound could be used in the future to develop biotechnological products for the control of phytopathogenic fungi.
Centro de Análise Proteômicas e Bioquímicas de Brasília, Universidade Católica de Brasília, Brasília, DF, Brazil.
Suzana M
SM
Ribeiro
Renato G
RG
Almeida
Camila A A
CA
Pereira
João S
JS
Moreira
Michelle F S
MF
Pinto
Antonio C
AC
Oliveira
Ilka M
IM
Vasconcelos
José T A
JT
Oliveira
Marcelo O
MO
Santos
Simoni C
SC
Dias
Octávio L
OL
Franco
D000418:Albumins / Q000737:chemistry*; D000890:Anti-Infective Agents / Q000737:chemistry* / Q000494:pharmacology*; D002175:Candida / Q000187:drug effects; D002176:Candida albicans / Q000187:drug effects; D041221:Candida glabrata / Q000187:drug effects; D020231:Colletotrichum / Q000187:drug effects; D003455:Cryptococcus neoformans / Q000187:drug effects; D004591:Electrophoresis, Polyacrylamide Gel; D008826:Microbial Sensitivity Tests; D029598:Passiflora / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry* / Q000494:pharmacology*; D012486:Salmonella typhimurium / Q000187:drug effects; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013211:Staphylococcus aureus / Q000187:drug effects
D000418:Albumins; D000890:Anti-Infective Agents; D010455:Peptides