Flow cytometry as a tool to determine the effects of cell wall-active antibiotics on vancomycin-susceptible and -resistant Enterococcus faecalis strains.
Flow cytometry and confocal microscopy were used to study the effects of vancomycin, daptomycin, telavancin, and PA1409, a new investigational vancomyquine, on the morphology, membrane potential, and permeability of glycopeptide-susceptible and -resistant Enterococcus faecalis strains. Daptomycin exerted the most pronounced effects on bacterial size and bacterial permeability against susceptible and resistant strains.
Chau
Françoise
F
EA3964, Faculté de Médecine Xavier Bichat, Université Paris 7, Paris, France.
Lefort
Agnès
A
Benadda
Samira
S
Dubée
Vincent
V
Fantin
Bruno
B
D000617:Aminoglycosides / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D017576:Daptomycin / Q000494:pharmacology*; D013293:Enterococcus faecalis / Q000187:drug effects*; D005434:Flow Cytometry; D000077427:Lipoglycopeptides; D018613:Microscopy, Confocal; D014640:Vancomycin / Q000494:pharmacology*
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D000077427:Lipoglycopeptides; D014640:Vancomycin; D017576:Daptomycin; C487637:telavancin