Novel Cellulase Screening and Optimal Production from the Wood Decaying Xylariaceae: Daldinia Species.
The highest cellulases production from Daldinia caldariorum 263 (D-263) was found among Daldinia eschscholzii and Daldinia childiae. Three cellulases, one xylanase and one β-glucosidase of the molecular weights 55, 43, 34, 30, and 105 kDa, respectively, were determined by zymographic sodium dodecyl sulfate polyacrylamide gel electrophoresis. From the N-terminal sequencing, the major cellulase CelA belonging to glycosyl hydrolase family 5 was determined. By following an orthogonal experiment design (L9), factors affecting the cultivation of D. caldariorum 263 are ranked as medium composition > temperature > pH ≥ FP (%). The optimum cultivation conditions for obtaining the best FPase (600 mU/ml) at 72 h are 150 rpm, 35 °C, pH 7, 0.2% soy peptone and 0.5% α-cellulose in minimal requirement medium. In comparison with Trichoderma reesei (ATCC26921) secreting 1,135 mU/ml of FPase after 6 days cultivation at pH 5, D. caldariorum 263 grew faster at 35 °C and produced the maximum FPase within 3 days at pH 7.
Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, China. yswu@xmu.edu.cn.
I-Son
IS
Ng
Department of Biotechnology, Southern Taiwan University, 1 Nan-Tai Street, Yung Kang City, Tainan County, 71005, Taiwan.
Po Ting
PT
Chen
Institute of Plant and Microbial Biology, Academia Sinica, Nankang, Taipei, 11529, Taiwan.
Yu-Ming
YM
Ju
Institute of Biochemical and Biomedical Engineering, Chang Gung University, Kwei-Shan, Tao-Yuan, 33302, Taiwan (Republic of China).
Shau-Wei
SW
Tsai
D002480:Cellulase / Q000378:metabolism / Q000737:chemistry; D014934:Wood / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D013696:Temperature; D013098:Xylariales / Q000201:enzymology / Q000378:metabolism; D005656:Fungal Proteins / Q000737:chemistry / Q000378:metabolism; D006999:Hypocreales
D002480:Cellulase; D005656:Fungal Proteins