Vibrational spectroscopy differentiates between multipotent and pluripotent stem cells.
Over the last few years, there has been an increased interest in the study of stem cells in biomedicine for therapeutic use and as a source for healing diseased or injured organs/tissues. More recently, vibrational spectroscopy has been applied to study stem cell differentiation. In this study, we have used both synchrotron based FTIR and Raman microspectroscopies to assess possible differences between human pluripotent (embryonic) and multipotent (adult mesenchymal) stem cells, and how O(2) concentration in cell culture could affect the spectral signatures of these cells. Our work shows that infrared spectroscopy of embryonic (pluripotent) and adult mesenchymal (multipotent) stem cells have different spectral signatures based on the amount of lipids in their cytoplasm (confirmed with cytological staining). Furthermore, O(2) concentration in cell culture causes changes in both the FTIR and Raman spectra of embryonic stem cells. These results show that embryonic stem cells might be more sensitive to O(2) concentration when compared to mesenchymal stem cells. While vibrational spectroscopy could therefore be of potential use in identifying different populations of stem cells further work is required to better understand these differences.
Pijanka
Jacek Klaudiusz
JK
Institute for Science and Technology in Medicine, Guy Hilton Research Centre, Keele University, Stoke on Trent, UK.
Kumar
Deepak
D
Dale
Tina
T
Yousef
Ibraheem
I
Parkes
Gary
G
Untereiner
Valérie
V
Yang
Ying
Y
Dumas
Paul
P
Collins
David
D
Manfait
Michel
M
Sockalingum
Ganesh Dhruvananda
GD
Forsyth
Nicholas Robert
NR
Sulé-Suso
Josep
J
D002478:Cells, Cultured; D006801:Humans; D008055:Lipids / Q000032:analysis; D039902:Multipotent Stem Cells / Q000737:chemistry* / Q000166:cytology; D039904:Pluripotent Stem Cells / Q000737:chemistry* / Q000166:cytology; D025341:Principal Component Analysis; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*; D013059:Spectrum Analysis, Raman / Q000379:methods*
D008055:Lipids