Non-coding small (micro) RNAs of Pseudomonas aeruginosa isolated from clinical isolates from adult patients with cystic fibrosis.
MicroRNAs are a class of small non-coding RNAs widely reported in eukaryotic multicellular organisms. In this study, a number of small non-coding micro (mi)RNA species in clinical isolates of prokaryote Pseudomonas aeruginosa were obtained from the sputum of adult patients with cystic fibrosis (CF) utilising a DynaExpress miRNA cloning kit, and five miRNAs of 16-47 nucleotides that were smaller than those encountered or described (80-100 nucleotides) previously in bacterial systems were described. This report presents data on these unknown cellular miRNAs cloned from P. aeruginosa isolates from CF patients. Adapting a computational miRNA prediction model that takes advantage of the highly conserved known miRNA hair pin stems regions, the results revealed that the fold structure of the microRNAs had a high homology to the recently reported human bacterial infection response (BiR)-related microRNA, mi-146, associated with the Toll-like receptor (TLR) family, which is the primary evolutionarily conserved sensors of pathogen-associated molecular patterns (PAMPs), and known to trigger host inflammatory and immune responses.
Rao
J R
JR
Molecular Diagnostics & Environmental Health, Agri-Food & Biosciehces Institute, Belfast, Northern Ireland, UK. jr.rao@afbini.gov.uk
Nelson
D
D
Moore
J E
JE
Millar
B C
BC
Goldsmith
C E
CE
Rendall
J
J
Elborn
J S
JS
D000328:Adult; D001483:Base Sequence; D003550:Cystic Fibrosis / Q000150:complications*; D006801:Humans; D035683:MicroRNAs / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D009894:Opportunistic Infections / Q000150:complications / Q000382:microbiology; D011552:Pseudomonas Infections / Q000150:complications* / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000235:genetics* / Q000302:isolation & purification; D012329:RNA, Bacterial / Q000302:isolation & purification*; D013183:Sputum / Q000382:microbiology
D035683:MicroRNAs; D012329:RNA, Bacterial