Liver antioxidant and plasma immune responses in juvenile golden grey mullet (Liza aurata) exposed to dispersed crude oil.
Dispersants are often used after oil spills. To evaluate the environmental cost of this operation in nearshore habitats, the experimental approach conducted in this study exposed juvenile golden grey mullets (Liza aurata) for 48 h to chemically dispersed oil (simulating, in vivo, dispersant application), to dispersant alone in seawater (as an internal control of chemically dispersed oil), to mechanically dispersed oil (simulating, in vivo, natural dispersion), to the water-soluble fraction of oil (simulating, in vivo, an oil slick confinement response technique) and to seawater alone (control condition). Biomarkers such as fluorescence of biliary polycyclic aromatic hydrocarbon (PAH) metabolites, total glutathione liver content, EROD (7-ethoxy-resorufin-O-deethylase) activity, liver antioxidant enzyme activities, liver lipid peroxidation and an innate immune parameter (haemolytic activity of the alternative complement pathway) were measured to assess the toxicity of dispersant application. Significant responses of PAH metabolites and total glutathione content of liver to chemically dispersed oil were found, when compared to water-soluble fraction of oil. As was suggested in other studies, these results highlight that priority must be given to oil slick confinement instead of dispersant application. However, since the same patterns of biomarker responses were observed for both chemically and mechanically dispersed oil, the results also suggest that dispersant application is no more toxic than the natural dispersion occurring in nearshore areas (due to, e.g. waves). The results of this study must, nevertheless, be interpreted cautiously since other components of nearshore habitats must be considered to establish a framework for dispersant use in nearshore areas.
Milinkovitch
Thomas
T
Littoral Environnement et Sociétés (LIENSs), UMR 6250, CNRS-Université de La Rochelle, La Rochelle, France. thomas.milinkovitch01@univ-lr.fr
Ndiaye
Awa
A
Sanchez
Wilfried
W
Le Floch
Stéphane
S
Thomas-Guyon
Hélène
H
D000704:Analysis of Variance; D000818:Animals; D015415:Biomarkers / Q000378:metabolism*; D003165:Complement System Proteins / Q000378:metabolism; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism; D004785:Environmental Pollutants / Q000633:toxicity*; D005978:Glutathione / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D010578:Petroleum / Q000633:toxicity*; D011084:Polycyclic Aromatic Hydrocarbons / Q000378:metabolism; D023701:Smegmamorpha / Q000276:immunology* / Q000378:metabolism*; D013050:Spectrometry, Fluorescence
D015415:Biomarkers; D004785:Environmental Pollutants; D010578:Petroleum; D011084:Polycyclic Aromatic Hydrocarbons; D003165:Complement System Proteins; D019363:Cytochrome P-450 CYP1A1; D005978:Glutathione