Identification of zRAP55,a gene preponderantly expressed in Stages I and II oocytes of zebrafish.
In an in silico search for gonand specific expressed genes, we have identified zRAP55 which is enriched in the ovary of zebrafish . zRAP55 encodes a protein of 382 amino acids with a highly conserved Lsm domain. zRAP55 protein shares more than 56% identities with that of other vertebrate species. RT-PCR results show that it is predominantly expressed in the ovary. In situ hybridization and immunohistochemistry studies reveal that zRAP55 is ubiquitously dispersed throughout the cytoplasm of stages I and II oocytes, whereas no expression is observed in stages III and IV oocytes. As an RNA associated protein, zRAP55 might function in the control of protein translation at the early stages of oogenesis in zebrafish.
Zhao
Cai-Lian
CL
Fisheries College, Huazhong Agricultural University, Wuhan 430070, China. pingsongs@gmail.com.
Yang
Qi-Wen
QW
Hu
Jia-Rui
JR
Ye
Ding
D
Gong
Wu-Ming
WM
Lu
Hai-Ying
HY
Xu
Zong-Yun
ZY
Zhang
Xun-Pu
XP
Song
Ping
P
D000595:Amino Acid Sequence; D000818:Animals; D005260:Female; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D009865:Oocytes / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D016601:RNA-Binding Proteins / Q000032:analysis / Q000737:chemistry / Q000235:genetics*; D011817:Rabbits; D016415:Sequence Alignment; D015027:Zebrafish / Q000235:genetics*; D029961:Zebrafish Proteins / Q000032:analysis / Q000737:chemistry / Q000235:genetics*
D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D029961:Zebrafish Proteins; C556272:lsm14b protein, zebrafish