-607 C/A polymorphism in the promoter of IL-18 gene is associated with 2 h post-loading plasma glucose level in Chinese.
The purpose of this study is to find out whether the -607 C/A polymorphism in IL-18 gene promoter will affect serum IL-18 concentrations and glucose metabolism in Chinese subjects. In 232 patients with impaired glucose regulation (IGR) or type 2 diabetes and 212 subjects of normal glucose regulation (NGR), -607 C/A polymorphism was analyzed by polymerase chain reaction with sequence specific primers. Serum IL-18 concentrations were determined by ELISA in 200 subjects. Compared with subjects with NGR, those with type 2 diabetes and IGR had significant higher IL-18 concentrations [114.4 (92.9-149.2) and 102.1 (67.5-138.2) vs. 77.3 (53.8-107.8) pg/ml, P = 0.0026 and P < 0.0001, respectively]. A significantly higher proportion of A/A genotype of -607 C/A polymorphism presented in patients with type 2 diabetes compared with subjects of NGR (23.8% vs. 10.9%, P = 0.005). Subjects with an A/A genotype also had higher 2 h post-loading plasma glucose (2 h-PPG) compared with C/A and C/C genotypes even after adjustments of age, sex and BMI [8.94 (7.55-12.3) vs. 7.80 (6.00-9.87) and 7.23 (5.66-8.99) mmol/l, P both < 0.05]. Multiple linear regression revealed that BMI (P < 0.0001) and 2 h-PPG (P = 0.019) were independently associated with IL-18 concentrations. In conclusion, subjects with IGR or type 2 diabetes had significantly higher concentrations of IL-18 than those with NGR. Genotype A/A of IL-18 gene promoter -607 C/A polymorphism was associated with higher prevalence of type 2 diabetes and 2 h post-loading plasma glucose level.
Department of Endocrinology and Metabolism, Shanghai Institute of Endocrinology and Metabolism, Shanghai Clinical Center for Endocrine and Metabolic Diseases, Rui-Jin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, China.
Yun
Y
Huang
Min
M
Xu
Jie
J
Hong
Weiqiong
W
Gu
Yufang
Y
Bi
Xiaoying
X
Li
D000328:Adult; D044466:Asian People / Q000235:genetics*; D001786:Blood Glucose / Q000378:metabolism*; D002681:China; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000235:genetics*; D005260:Female; D005947:Glucose / Q000008:administration & dosage; D006801:Humans; D020382:Interleukin-18 / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic
D001786:Blood Glucose; D020382:Interleukin-18; D005947:Glucose