Increasing fatty acid production in E. coli by simulating the lipid accumulation of oleaginous microorganisms.
Unlike many oleaginous microorganisms, E. coli only maintains a small amount of natural lipids in cells, impeding its utility to overproduce fatty acids. In this study, acetyl-CoA carboxylase (ACC) from Acinetobacter calcoaceticus was expressed in E. coli to redirect the carbon flux to the generation of malonyl-CoA, which resulted in a threefold increase in intracellular lipids. Moreover, providing a high level of NADPH by overexpressing malic enzyme and adding malate to the culture medium resulted in a fourfold increase in intracellular lipids (about 197.74 mg/g). Co-expression of ACC and malic enzyme resulted in 284.56 mg/g intracellular lipids, a 5.6-fold increase compared to the wild-type strain. This study provides some attractive strategies for increasing lipid production in E. coli by simulating the lipid accumulation of oleaginous microorganisms, which could aid the development of a prokaryotic fatty acid producer.
Meng
Xin
X
Key Laboratory of Biofuel, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, China.
Yang
Jianming
J
Cao
Yujin
Y
Li
Liangzhi
L
Jiang
Xinglin
X
Xu
Xin
X
Liu
Wei
W
Xian
Mo
M
Zhang
Yingwei
Y
D000103:Acetyl-CoA Carboxylase / Q000235:genetics / Q000378:metabolism*; D016954:Acinetobacter calcoaceticus / Q000235:genetics / Q000378:metabolism; D001709:Biotechnology; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism*; D008055:Lipids / Q000096:biosynthesis; D008291:Malate Dehydrogenase; D008293:Malates / Q000378:metabolism; D008316:Malonyl Coenzyme A / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D014169:Transformation, Bacterial
D029968:Escherichia coli Proteins; D005227:Fatty Acids; D008055:Lipids; D008293:Malates; D008316:Malonyl Coenzyme A; D009249:NADP; C030298:malic acid; D008291:Malate Dehydrogenase; C030574:malate dehydrogenase (decarboxylating); D000103:Acetyl-CoA Carboxylase