A combined biomarker pattern improves the discrimination of lung cancer.
A total of 227 sera were analysed using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS) to find novel serum biomarkers for lung cancer. The results showed that the 11.53, 11.70, 13.78, 13.90 and 14.07 k m/z peaks identified as native serum amyloid A (SAA), SAA with N-terminal Arg cleaved, native transthyretin (TTR) and its two variants significantly differentiated lung cancer sera from normal control sera (p <0.01). A 'biomarker pattern' combining SAA and TTR was tested to distinguish lung cancer patients from normal control individuals, and the diagnostic positive rate of lung cancer was improved to 91.6%.
Key Laboratory for Cell Proliferation and Regulation Biology Ministry of Education, Beijing Normal University, China.
Liyun
L
Liu
Jifu
J
Liu
Yinping
Y
Wang
Songwei
S
Dai
Xiaomin
X
Wang
Shanshan
S
Wu
Jianing
J
Wang
Lingyun
L
Huang
Xueyuan
X
Xiao
Dacheng
D
He
D000230:Adenocarcinoma / Q000097:blood / Q000175:diagnosis; D000077192:Adenocarcinoma of Lung; D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000097:blood; D002294:Carcinoma, Squamous Cell / Q000097:blood / Q000175:diagnosis; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D047468:Immunoprecipitation; D008175:Lung Neoplasms / Q000097:blood* / Q000175:diagnosis*; D008297:Male; D008875:Middle Aged; D011228:Prealbumin / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D040081:Protein Array Analysis; D012680:Sensitivity and Specificity; D000685:Serum Amyloid A Protein / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D055752:Small Cell Lung Carcinoma / Q000097:blood / Q000175:diagnosis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D015415:Biomarkers; D011228:Prealbumin; D000685:Serum Amyloid A Protein