Flow cytometry as a tool to determine the effects of cell wall-active antibiotics on vancomycin-susceptible and -resistant Enterococcus faecalis strains.
Flow cytometry and confocal microscopy were used to study the effects of vancomycin, daptomycin, telavancin, and PA1409, a new investigational vancomyquine, on the morphology, membrane potential, and permeability of glycopeptide-susceptible and -resistant Enterococcus faecalis strains. Daptomycin exerted the most pronounced effects on bacterial size and bacterial permeability against susceptible and resistant strains.
EA3964, Faculté de Médecine Xavier Bichat, Université Paris 7, Paris, France.
Françoise
F
Chau
Agnès
A
Lefort
Samira
S
Benadda
Vincent
V
Dubée
Bruno
B
Fantin
D000617:Aminoglycosides / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D017576:Daptomycin / Q000494:pharmacology*; D013293:Enterococcus faecalis / Q000187:drug effects*; D005434:Flow Cytometry; D000077427:Lipoglycopeptides; D018613:Microscopy, Confocal; D014640:Vancomycin / Q000494:pharmacology*
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D000077427:Lipoglycopeptides; D014640:Vancomycin; D017576:Daptomycin; C487637:telavancin