Proximity of the start codon to a leaderless mRNA's 5' terminus is a strong positive determinant of ribosome binding and expression in Escherichia coli.
An AUG start codon is an important determinant of ribosome binding and expression of leaderless mRNAs in Escherichia coli. Using reporter constructs encoding mRNAs where the AUG start codon is preceded by untranslated leaders of various length and sequence, we find that close proximity of the start codon to the 5' terminus and the leader sequence are strong determinants of both ribosome binding and expression.
Krishnan
Karthik M
KM
Department of Microbiology, Miami University, Oxford, Ohio 45056, USA.
Van Etten
William J
WJ
Janssen
Gary R
GR
D020121:5' Untranslated Regions; D018387:Codon, Initiator; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D011485:Protein Binding; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D012269:Ribosomal Proteins / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism*
D020121:5' Untranslated Regions; D018387:Codon, Initiator; D012333:RNA, Messenger; D012269:Ribosomal Proteins