Ultrasensitive nucleic acid detection using confocal laser scanning microscope with high crystalline silver dendrites.
A new kind of silver micro-dendrites with no surfactants protected has been synthesized for the separation and detection of DNA merely by earth gravity as the density of silver is larger than that of water. Through this approach, the DNA of human T-lymphotropic virus type I (HTLV-I) can be detected down to 10 pM with the detection range from 10 pM to 100 nM.
Yang
Xuan
X
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Changchun 130022, Jilin, PR China.
Sun
Xuping
X
Lv
Zhaozi
Z
Guo
Weiwei
W
Du
Yan
Y
Wang
Erkang
E
D003460:Crystallization; D004279:DNA, Viral / Q000032:analysis*; D050091:Dendrimers / Q000737:chemistry*; D015368:Human T-lymphotropic virus 1 / Q000737:chemistry*; D018613:Microscopy, Confocal; D012834:Silver / Q000737:chemistry*
D004279:DNA, Viral; D050091:Dendrimers; D012834:Silver