[Isolation and identification of a methanogen from the high temperature oil reservoir water].
To explore new microbial resources in deep subsurface oil reservoirs, strain DL-7 was isolated with Hungate technology from oil reservoir water sampled from Dagang oilfield, China. Physiological and biochemical examinations showed that H2/CO2 is the unique substrate of the strain, which cannot metabolize formate, methanol, trimethylamine, acetate and other secondary alcohols. The optimum growth conditions were further identified to be 60 degrees C, pH 7.0-7.5 and 0.25% NaCl. Moreover, the strain cannot grow without yeast extract. Analysis of its 16S rRNA sequence indicated that a similarity of 99.7% presents between the strain and the model species M. marburgensis DSM2133T (X15364).
Key Laboratory of Energy Microbiology and Application, Chengdu 610041, China.
Haichang
H
Liu
Guihong
G
Lan
Quanquan
Q
Liu
Wenjing
W
Zhang
Yi
Y
Cao
Yu
Y
Deng
Hui
H
Zhang
D006358:Hot Temperature; D017013:Methanobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D000432:Methanol / Q000378:metabolism; D019638:Methanomicrobiaceae / Q000235:genetics / Q000302:isolation & purification; D010578:Petroleum / Q000382:microbiology*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D014871:Water Microbiology
D010578:Petroleum; D012336:RNA, Ribosomal, 16S; D000432:Methanol