Carbapenem-hydrolyzing GES-type extended-spectrum beta-lactamase in Acinetobacter baumannii.
Acinetobacter baumannii isolate AP was recovered from a bronchial lavage of a patient hospitalized in Paris, France. A. baumannii AP was resistant to all β-lactams, including carbapenems, and produced the extended-spectrum β-lactamase (ESBL) GES-14, which differs from GES-1 by two substitutions, Gly170Ser and Gly243Ala. Cloning of the bla(GES-14) gene followed by its expression in Escherichia coli showed that GES-14 compromised significantly the efficacy of all β-lactams, including cephalosporins, aztreonam, and carbapenems. The carbapenemase activity of purified GES-14 was confirmed by kinetic studies. The bla(GES-14) gene was located into a class 1 integron structure and located onto a ca. 95-kb self-transferable plasmid. This study identified a very broad-spectrum β-lactamase in A. baumannii.
Service de Bactériologie-Virologie, INSERM U914, Emerging Resistance to Antibiotics, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine et Université Paris-Sud, Hôpital de Bicêtre, Le Kremlin-Bicêtre, France.
Rémy A
RA
Bonnin
Patrice
P
Nordmann
Anaïs
A
Potron
Hervé
H
Lecuyer
Jean-Ralph
JR
Zahar
Laurent
L
Poirel
D040981:Acinetobacter baumannii / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D015780:Carbapenems / Q000378:metabolism* / Q000494:pharmacology; D019818:Clavulanic Acid / Q000494:pharmacology; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D008826:Microbial Sensitivity Tests; D008957:Models, Genetic; D008969:Molecular Sequence Data; D010397:Penicillanic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D016133:Polymerase Chain Reaction; D000078142:Tazobactam; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D015780:Carbapenems; D019818:Clavulanic Acid; D010397:Penicillanic Acid; D001618:beta-Lactamases; D000078142:Tazobactam