Neural stem cells differentiation ability of human umbilical cord mesenchymal stromal cells is not altered by cryopreservation.
Human umbilical mesenchymal stem cells (HUMSCs) have potential therapeutic use in the recovery of central nervous system injury for their ability to differentiate into neural stem cells. However, for transformed HUMSCs to be constantly available for use during surgery a reliable method of cell storage is necessary. The present study aimed to determine whether a simple method of cryopreservation by slow cooling with Me(2)SO had an effect on the proliferation, secretion and differentiation capacities of HUMSCs. These results demonstrate that cryopreservation has no effect on the phenotype, cell cycle, cell proliferation and the ability to secret neurotrophins. Non-cryopreserved and cryopreserved HUMSCs showed the similar ability to differentiate into neural stem-like cells. There results show that cryopreservation by slow cooling with Me(2)SO is effective to retain the proliferation and neural differentiation ability of HUMSCs, cryopreserved HUMSCs maybe very useful for future clinical applications in neural regenerative medicine.
Department of Neurosurgery, The Military General Hospital of PLA, Beijing, China.
Hong-Tian
HT
Zhang
Hong
H
Chen
Hao
H
Zhao
Yi-Wu
YW
Dai
Ru-Xiang
RX
Xu
D000704:Analysis of Variance; D002452:Cell Count; D002453:Cell Cycle / Q000502:physiology; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D015925:Cryopreservation / Q000379:methods*; D005786:Gene Expression Regulation / Q000502:physiology; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D020932:Nerve Growth Factor / Q000235:genetics / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D058953:Neural Stem Cells / Q000502:physiology*; D014470:Umbilical Cord / Q000166:cytology*
D009419:Nerve Tissue Proteins; D020932:Nerve Growth Factor