Transcriptional regulation of extracellular copper zinc superoxide dismutase from white shrimp Litopenaeus vannamei following Vibrio alginolyticus and WSSV infection.
The cDNA encoding an extracellular copper zinc superoxide dismutase (LvECSOD) was cloned from the hepatopancreas of white shrimp Litopenaeus vannamei. It consisted of 915 bp nucleotides with an open reading frame corresponding to a deduced protein of 178 amino acids. The LvECSOD contains a putative signal peptide of 16 amino acids, two potential N-linked glycosylation sites (N(115)GTA and N(135)ITG) and a copper zinc superoxide dismutase family signature sequence (G(162)NAGaRvACctI(173)). It was found that four copper binding sites, four zinc binding sites and two cysteines involving in the formation of the disulfide bridge were conserved in the protein. LvECSOD shared 33-58% identity to ECSODs from other organisms. Expression analysis revealed that LvECSOD mRNA was widely distributed in all the tissues examined. When the shrimp challenged with Vibrio alginolyticus or white spot syndrome virus (WSSV), expression of LvECSOD mRNA in the hepatopancreas and hemocytes was mediated responsively. Our results suggested that LvECSOD was implicated in the immune response induced by V. alginolyticus and WSSV.
Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, College of Life Science, South China Normal University, Guangzhou 510631, PR China.
Jianxiao
J
Tian
Juan
J
Chen
Dan
D
Jiang
Shaoan
S
Liao
Anli
A
Wang
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015971:Gene Expression Regulation, Enzymologic / Q000276:immunology*; D054884:Host-Pathogen Interactions; D008969:Molecular Sequence Data; D033561:Penaeidae / Q000201:enzymology* / Q000382:microbiology / Q000821:virology; D058974:Phylogeography; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic; D044149:Vibrio alginolyticus / Q000502:physiology*; D046848:White spot syndrome virus 1 / Q000502:physiology*
D012333:RNA, Messenger; D013482:Superoxide Dismutase