DosS responds to a reduced electron transport system to induce the Mycobacterium tuberculosis DosR regulon.
The DosR regulon in Mycobacterium tuberculosis is involved in respiration-limiting conditions, its induction is controlled by two histidine kinases, DosS and DosT, and recent experimental evidence indicates DosS senses either molecular oxygen or a redox change. Under aerobic conditions, induction of the DosR regulon by DosS, but not DosT, was observed after the addition of ascorbate, a powerful cytochrome c reductant, demonstrating that DosS responds to a redox signal even in the presence of high oxygen tension. During hypoxic conditions, regulon induction was attenuated by treatment with compounds that occluded electron flow into the menaquinone pool or decreased the size of the menaquinone pool itself. Increased regulon expression during hypoxia was observed when exogenous menaquinone was added, demonstrating that the menaquinone pool is a limiting factor in regulon induction. Taken together, these data demonstrate that a reduced menaquinone pool directly or indirectly triggers induction of the DosR regulon via DosS. Biochemical analysis of menaquinones upon entry into hypoxic/anaerobic conditions demonstrated the disappearance of the unsaturated species and low-level maintenance of the mono-saturated menaquinone. Relative to the unsaturated form, an analog of the saturated form is better able to induce signaling via DosS and rescue inhibition of menaquinone synthesis and is less toxic. The menaquinone pool is central to the electron transport system (ETS) and therefore provides a mechanistic link between the respiratory state of the bacilli and DosS signaling. Although this report demonstrates that DosS responds to a reduced ETS, it does not rule out a role for oxygen in silencing signaling.
Honaker
Ryan W
RW
Department of Microbiology, University of Colorado Denver, School of Medicine, P18-9115, 12800 East 19th Avenue, PO Box 6511, Aurora, Colorado 80045, USA.
Dhiman
Rakesh K
RK
Narayanasamy
Prabagaran
P
Crick
Dean C
DC
Voskuil
Martin I
MI
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins; D004579:Electron Transport / Q000502:physiology; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D008967:Molecular Biology; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000378:metabolism*; D010101:Oxygen Consumption / Q000502:physiology*; D006656:Protamine Kinase / Q000235:genetics / Q000378:metabolism*; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D024482:Vitamin K 2 / Q000737:chemistry / Q000378:metabolism
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C542863:DosR protein, Mycobacterium tuberculosis; D024482:Vitamin K 2; D011494:Protein Kinases; C506260:DevS protein, Mycobacterium tuberculosis; D006656:Protamine Kinase