Immunohistochemical analysis of paraoxonases-1 and 3 in human atheromatous plaques.
BACKGROUND
The paraoxonase (PON) enzyme family comprising PON1, PON2 and PON3 are antioxidant enzymes that degrade bioactive oxidised lipids and are thus antiatherogenic.


METHODS
We investigated the localisation of the PON proteins during the development of atherosclerosis by immunohistochemical analysis.


RESULTS
In normal aortas, PON1 and PON3 were localised to smooth muscle cells (SMC) and endothelial cells. PON3 staining was stronger than that of PON1. During atherosclerosis development, SMC staining for PON1 and PON3 was greatly reduced, while macrophage staining for both proteins increased with PON1 predominating. Macrophage staining for PON1 and PON3 was significantly and positively related to the amount of aortic inflammation (both P<0·001).


CONCLUSIONS
Our data add support to the growing body of evidence for a cellular protective effect of PON1 and PON3 against the proinflammatory/proatherosclerotic effects of lipid peroxidation.
Centre de Recerca Biomèdica, Hospital Universitari de Sant Joan, Institut d'Investigacions Sanitàries Pere Virgili, Universitat Rovira i Virgili, Reus, Catalunya, Spain.
Judit
J
Marsillach
Jordi
J
Camps
Raul
R
Beltran-Debón
Anna
A
Rull
Gerard
G
Aragones
Carmen
C
Maestre-Martínez
Fàtima
F
Sabench
Mercè
M
Hernández
Daniel D
DD
Castillo
Jorge
J
Joven
Mike
M
Mackness
Bharti
B
Mackness
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D043303:Aryldialkylphosphatase / Q000378:metabolism*; D018929:Cell Culture Techniques; D002648:Child; D005260:Female; D006801:Humans; D015227:Lipid Peroxidation / Q000502:physiology; D008264:Macrophages / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress / Q000502:physiology; D058226:Plaque, Atherosclerotic / Q000473:pathology* / Q000503:physiopathology; D055815:Young Adult
D043303:Aryldialkylphosphatase