Comparison of isoelectric focusing and polymerase chain reaction for the detection of β-lactamases.
Extended spectrum β-lactamases (ESBLs) have been observed in virtually all the species of family Enterobacteriaceae. Threat posed by antibiotic resistance because of ESBLs is more serious as a number of technical problems are associated with the detection of these enzymes. Although a number of detection methods have been designed for ESBLs, every method has its own benefits and shortcomings as well. In earlier days, isoelectric focusing (IEF) was used as the gold standard for ESBL detection. This study was undertaken to compare IEF with polymerase chain reaction, a method which has been extensively used for ESBL detection these days.
Sharma
J
J
Department of Microbiology, Dr. HSJ Institute of Dental Sciences and Hospital, Panjab University, Chandigarh, India. contactjyotisharma@yahoo.co.in
Ray
P
P
Sharma
M
M
Ghosh
S
S
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D006801:Humans; D007525:Isoelectric Focusing / Q000379:methods; D007711:Klebsiella pneumoniae / Q000201:enzymology* / Q000235:genetics; D008826:Microbial Sensitivity Tests / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism; D047090:beta-Lactams
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D047090:beta-Lactams; D001618:beta-Lactamases