Hydrolytic enzyme of cellulose for complex formulation applied research.
To improve the enzymatic hydrolytic efficiency and reduce the supplementation of enzymes, the mixture designed experimental approach was used to optimize the composition of enzyme mixture and promote the hydrolysis of ball-milled corn stover. From the experimental results, a synergistic effect was found when combinations of the three enzymes, two kinds of cellulases and a kind of xylanase, were used. The optimal hydrolysis of pretreated corn stover accorded with enzymes activity ration of FPU/CMCase/β-glucosidase/xylanase = 4.4:1:75:829, and the hydrolysis efficiency of corn stover increased significantly compared with using individual enzyme. The results indicated that the mixture design experiment could be an effective tool for optimized enzyme mixture for lignocellulose hydrolysis.
State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, No. 5 Xinmofan Road, Nanjing, People's Republic of China.
Zeng-Xiang
ZX
Lin
Hong-Man
HM
Zhang
Xiao-Jun
XJ
Ji
Jing-Wen
JW
Chen
He
H
Huang
D000821:Animal Feed; D001709:Biotechnology / Q000379:methods*; D043364:Endo-1,4-beta Xylanases / Q000378:metabolism*; D005936:Glucans / Q000378:metabolism; D006868:Hydrolysis; D008031:Lignin / Q000378:metabolism*; D014990:Xylans / Q000378:metabolism; D003313:Zea mays / Q000737:chemistry / Q000378:metabolism; D001617:beta-Glucosidase / Q000378:metabolism*
D005936:Glucans; D014990:Xylans; C036909:lignocellulose; D008031:Lignin; D001617:beta-Glucosidase; D043364:Endo-1,4-beta Xylanases