Transgenic labeling of the zebrafish pronephric duct and tubules using a promoter from the enpep gene.
In recent years the zebrafish has become a popular model system to study organ development and disease. To facilitate these studies, genetic tools are required which allow to modify and manipulate gene expression in organs of interest. Here we describe a zebrafish 2kb glutamyl aminopeptidase (enpep) promoter fragment, and show that it can drive gene expression specifically in the kidney during early and late development. We established a stable transgenic line using this promoter fragment that has specific GFP expression in pronephric ducts and tubules starting at 20h post-fertilization.
Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA, USA.
Christoph
C
Seiler
Michael
M
Pack
D000818:Animals; D030801:Animals, Genetically Modified; D004625:Embryo, Nonmammalian / Q000378:metabolism; D043384:Glutamyl Aminopeptidase / Q000235:genetics*; D007668:Kidney / Q000196:embryology* / Q000378:metabolism; D007684:Kidney Tubules / Q000196:embryology / Q000378:metabolism; D011401:Promoter Regions, Genetic; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics* / Q000378:metabolism
D043384:Glutamyl Aminopeptidase