Immuno-pillar chip: a new platform for rapid and easy-to-use immunoassay.
We present a new rapid and easy-to-use immunoassay chip which we have named the immuno-pillar chip. It has hydrogel pillars, fabricated inside a microchannel, with many antibody molecules immobilized onto 1 µm diameter polystyrene beads. To evaluate the chip performance, we applied it to the sandwich assay of C-reactive protein (CRP), α-fetoprotein (AFP) and prostate-specific antigen (PSA), a cardiac and inflammation marker, tumors and prostate cancer markers, respectively. For detection of disease markers, we confirmed the chip provides rapid analysis (total assay time of about 4 min) with high sensitivity, it is easy-to-use (no special skills are needed), and it uses small volumes of the sample and reagent (0.25 µL each). Moreover, multiplex assay for three biomarkers was also possible. Additionally, the immuno-pillar chip has a big advantage of having hardly any influence on the assay results even if the introduction quantities of the sample or reagents are different.
Department of Applied Chemistry, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8603, Japan.
Mai
M
Ikami
Ayako
A
Kawakami
Masaya
M
Kakuta
Yukihiro
Y
Okamoto
Noritada
N
Kaji
Manabu
M
Tokeshi
Yoshinobu
Y
Baba
D000818:Animals; D014408:Biomarkers, Tumor; D002097:C-Reactive Protein / Q000032:analysis; D002138:Calibration; D002621:Chemistry / Q000379:methods; D006801:Humans; D020100:Hydrogels / Q000737:chemistry; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods*; D007249:Inflammation; D008297:Male; D046228:Microarray Analysis / Q000295:instrumentation* / Q000379:methods*; D008956:Models, Chemical; D011137:Polystyrenes / Q000737:chemistry; D017430:Prostate-Specific Antigen / Q000096:biosynthesis; D000509:alpha-Fetoproteins / Q000032:analysis
D014408:Biomarkers, Tumor; D020100:Hydrogels; D011137:Polystyrenes; D000509:alpha-Fetoproteins; D002097:C-Reactive Protein; D017430:Prostate-Specific Antigen