The liposome PCR assay is more sensitive than the Vibrio cholerae enterotoxin and Escherichia coli heat-labile enterotoxin reversed passive latex agglutination test at detecting cholera toxin in feces and water.
Practical detection of cholera toxin (CT) by a liposome PCR (LPCR) immunoassay was compared to that of an established V. cholerae enterotoxin and Escherichia coli heat-labile enterotoxin reversed passive latex agglutination (VET-RPLA) assay. LPCR detected CT in the range of 10 pg/ml to 100 ng/ml in simulated feces and environmental water. Detection by VET-RPLA required at least 4 to 19 ng/ml CT.
Department of Biophysics, Armed Forces Institute of Pathology, 810 Vermont Avenue, N.W., Washington, DC 20420, USA.
David L
DL
Evers
Junkun
J
He
Jeffrey T
JT
Mason
Timothy J
TJ
O'Leary
D001427:Bacterial Toxins / Q000032:analysis; D002772:Cholera Toxin / Q000032:analysis*; D004768:Enterotoxins / Q000032:analysis; D029968:Escherichia coli Proteins / Q000032:analysis; D005243:Feces / Q000737:chemistry*; D006801:Humans; D007118:Immunoassay / Q000379:methods; D007841:Latex Fixation Tests; D008081:Liposomes; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D014867:Water / Q000032:analysis*
D001427:Bacterial Toxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D008081:Liposomes; D014867:Water; D002772:Cholera Toxin; C027538:heat-labile enterotoxin, E coli