Digital self-referencing quantitative phase microscopy by wavefront folding in holographic image reconstruction.
A completely numerical method, named digital self-referencing holography, is described to easily accomplish a quantitative phase microscopy for microfluidic devices by a digital holographic microscope. The approach works through an appropriate numerical manipulation of the retrieved complex wavefront. The self-referencing is obtained by folding the retrieved wavefront in the image plane. The folding operation allows us to obtain the correct phase map by subtracting from the complex region of interest a flat area outside the microfluidic channel. To demonstrate the effectiveness of the method, quantitative phase maps of bovine spermatozoa and in vitro cells are retrieved.
Coppola
G
G
CNR—Istituto per la Microelettronica e Microsistemi, Via Pietro Castellino 111, 80131—Napoli, Italy. giuseppe.coppola@cnr.it
Di Caprio
G
G
Gioffré
M
M
Puglisi
R
R
Balduzzi
D
D
Galli
A
A
Miccio
L
L
Paturzo
M
M
Grilli
S
S
Finizio
A
A
Ferraro
P
P
D016475:3T3 Cells; D000465:Algorithms; D000818:Animals; D002417:Cattle; D004867:Equipment Design / Q000379:methods; D006696:Holography / Q000295:instrumentation / Q000379:methods*; D007089:Image Enhancement / Q000295:instrumentation / Q000379:methods*; D007090:Image Interpretation, Computer-Assisted / Q000295:instrumentation / Q000379:methods*; D007091:Image Processing, Computer-Assisted / Q000379:methods; D008297:Male; D051379:Mice; D008853:Microscopy / Q000295:instrumentation / Q000379:methods; D008858:Microscopy, Phase-Contrast / Q000295:instrumentation / Q000379:methods*; D012815:Signal Processing, Computer-Assisted; D013094:Spermatozoa / Q000166:cytology