Mapping of titanium particles in peri-implant oral mucosa by laser ablation inductively coupled plasma mass spectrometry and high-resolution optical darkfield microscopy.
The present study examines the quantity, size, element signatures and distribution of titanium particles in normal oral mucosal tissue and in oral mucosa exposed to a titanium implant. Tissue samples from six healthy patients were obtained by a full thickness biopsy taken from the edge of the oral mucosa when inserting a titanium dental implant. At the abutment insertion 6 months later, a punch test biopsy of oral mucosa was taken over the implant site. Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP-MS) is a sensitive and specific multi-element microanalytical technique that demonstrated the presence of Ti particles in the tissue adjacent to implant cover-screws. The epithelial part of the control samples revealed more particles than the corresponding area of the test samples, consisting partly of newly formed scar tissue. High-Resolution Optical Darkfield Microscope (HR-ODM) confirmed the presence of particles in both the control and the test samples. The combination of LA-ICP-MS and HR-ODM appears to be a powerful combination for detection of particles in oral tissues; optical microscopy provides an overview with histological references, whereas LA-ICP-MS identifies the chemical composition.
Department of Clinical Dentistry, Faculty of Medicine and Dentistry, University of Bergen, Bergen, Norway.
R S
RS
Flatebø
P J
PJ
Høl
K N
KN
Leknes
J
J
Kosler
S A
SA
Lie
N R
NR
Gjerdet
D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D003758:Dental Implantation, Endosseous; D015921:Dental Implants; D005260:Female; D006801:Humans; D007554:Isotopes / Q000032:analysis; D053844:Lasers, Solid-State; D057230:Limit of Detection; D016015:Logistic Models; D008297:Male; D008853:Microscopy / Q000379:methods; D008875:Middle Aged; D009061:Mouth Mucosa / Q000737:chemistry*; D010316:Particle Size; D058626:Plasma Gases; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation / Q000379:methods*; D014025:Titanium / Q000032:analysis*
D015921:Dental Implants; D007554:Isotopes; D058626:Plasma Gases; D014025:Titanium