Chiral separation of sertraline with microemulsion electrokinetic chromatography on a polymer/β-cyclodextrin assembling molecular film modified capillary.
A capillary modified by assembling a molecular film was presented for the chiral separation of sertraline by microemulsion electrokinetic chromatography. The assembling molecular film was constructed with poly(diallyldimethylammonium-chloride) and β-cyclodextrin via inclusion complexation. The separation efficiency of cis-trans isomers and enantiomers of sertraline was improved with a running microemulsion that contained the acetonitrile, sodium dodecyl sulfate, n-butanol and n-hexane buffered with sodium tetraborate. The baseline separation of four sertraline cis-trans isomers and enantiomers was achieved under the optimum conditions. The detection limit for isomers and enantiomers of sertraline (1S,4S, 1R,4R, 1S,4R, 1R,4S) was 0.15, 0.15, 0.30, 0.30 mg/L, respectively. The mechanism of chiral separation was studied and it could be applied for the determination of commercial Zoloft tablet samples satisfactorily.
Institute of Analytical Chemistry, Department of Chemistry, Dushu Lake Campus, Suzhou University, Suzhou 215123, P. R. China.
Hongying
H
Cheng
Baoku
B
He
Qianli
Q
Zhang
Yifeng
Y
Tu
D000928:Antidepressive Agents / Q000032:analysis* / Q000737:chemistry; D002021:Buffers; D053801:Capillary Electrochromatography; D002845:Chromatography; D004586:Electrophoresis; D006863:Hydrogen-Ion Concentration; D007536:Isomerism; D011108:Polymers; D020280:Sertraline / Q000032:analysis* / Q000737:chemistry; D013237:Stereoisomerism; D047392:beta-Cyclodextrins / Q000378:metabolism
D000928:Antidepressive Agents; D002021:Buffers; D011108:Polymers; D047392:beta-Cyclodextrins; C031215:betadex; D020280:Sertraline