Mesenchymal stem cells' interaction with skin: wound-healing effect on fibroblast cells and skin tissue.
Mesenchymal stem cells (MSCs) are multipotent progenitor cells with the ability to secrete growth factors. Because wound healing is associated with fibroblast cells and extracellular matrix (ECM) in the dermis and epidermis, we used fibroblast cells to resolve the question of whether or not MSCs regulate wound healing in vitro via a regenerative function. Using a cell proliferation assay, we demonstrated that conditioned media (CM) obtained from MSCs significantly enhanced the cell survival ability of fibroblast cells. Moreover, by measurement of mRNA and protein, we observed that CM also promoted the production or secretion of collagen, elastin, and fibronectin. To better understand the effects of ECM-related wound healing, we measured the level of collagen-degradative enzyme (matrix metalloprotease-1), and observed that CM suppressed matrix metalloprotease-1 expression. For the determination of oxidative stress, which has an influence on wound healing, we performed the superoxide dismutase and glutathione peroxidase assays; our results suggested that CM inhibited the oxidative stress of fibroblast cells. In order to widely investigate the wound-healing effects of MSCs, we performed in vivo experiments, and observed that MSCs stimulated wound healing. In summary, the results of this study suggest that MSCs inhibit the loss of fibroblast cells and ECM, and accumulation of oxidative stress. We found that MSCs stimulate wound healing in vitro and in vivo, suggesting that MSCs have the potential to enhance wound healing.
Department of Biological Science, Gachon University of Medicine and Science, Korea.
Young Keul
YK
Jeon
Yun Ho
YH
Jang
Dong Ryeol
DR
Yoo
Si Na
SN
Kim
Sang Koo
SK
Lee
Myeong Jin
MJ
Nam
D000818:Animals; D002465:Cell Movement; D049109:Cell Proliferation; D003094:Collagen / Q000378:metabolism; D017077:Culture Media, Conditioned; D004549:Elastin / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism*; D005353:Fibronectins / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D018384:Oxidative Stress; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000166:cytology* / Q000293:injuries; D013482:Superoxide Dismutase / Q000378:metabolism; D014945:Wound Healing
D017077:Culture Media, Conditioned; D005353:Fibronectins; D003094:Collagen; D004549:Elastin; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D020781:Matrix Metalloproteinase 1