Molecular characterization and genetic organization of the BamHI-C fragment of Clostera anachoreta granulovirus.
The BamHI-C restriction fragment of ClanGV genome was cloned and sequenced. Sequence analysis indicated that this region contains five important baculovirus homologous genes (chitinase, cathepsin, gp37, p49 and odv-e18) and one ORF unique to ClanGV genome. The genes, located within this restriction fragment, were compared with homologues in other baculoviruses. Comparison results indicated that ClanGV, Cydia pomonella GV and Pieris rapae GV have similar arrangement and orientation of the homologous genes. The ORFs related to these five genes were analyzed.
College of Life Sciences, Henan Agricultural University, 95 Wenhua Road, Zhengzhou, China.
Zhenpu
Z
Liang
Xiaoxia
X
Zhang
Xiaohui
X
Chen
D000818:Animals; D002403:Cathepsins / Q000235:genetics; D002688:Chitinases / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics*; D016679:Genome, Viral; D025722:Granulovirus / Q000201:enzymology / Q000235:genetics*; D009036:Moths / Q000821:virology; D016366:Open Reading Frames; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000235:genetics
D004279:DNA, Viral; D014764:Viral Proteins; D002688:Chitinases; D002403:Cathepsins