In vivo NIR imaging with CdTe/CdSe quantum dots entrapped in PLGA nanospheres.
Luminescent near-infrared (NIR) CdTe/CdSe QDs were synthesized and encapsulated in poly(lactic-co-glycolic acid) (PLGA) nanospheres to prepare stable and biocompatible QDs-loaded nanospheres for in vivo imaging. QDs were encapsulated with PLGA nanospheres by a solid dispersion method and optimized to have high fluorescence intensity for in vivo imaging detection. The resultant QDs-loaded PLGA nanospheres were characterized by various analytical techniques such as UV-Vis measurement, dynamic light scattering (DLS), fluorescence spectroscopy, and transmission electron microscopy (TEM). Finally, we evaluated toxicity and body distribution of QDs loaded in PLGA nanospheres in vitro and in vivo, respectively. From the results, the QDs loaded in PLGA nanospheres were spherical and showed a diameter range of 135.0-162.3 nm in size. The QD nanospheres increased their stability against photooxidation and photobleaching, which have the high potential for applications in biomedical imaging. We have also attained non-invasive in vivo imaging with light photons, representing an intriguing avenue for obtaining biological information by the use of NIR light.
Department of Chemical and Biological Engineering, Chungju National University, Chungbuk 380-702, Republic of Korea.
Jin Soo
JS
Kim
Kwang Jae
KJ
Cho
Thanh Huyen
TH
Tran
Md
M
Nurunnabi
Tae Hyun
TH
Moon
Suk Min
SM
Hong
Yong-kyu
YK
Lee
D000818:Animals; D019187:Cadmium Compounds / Q000737:chemistry; D045744:Cell Line, Tumor; D002470:Cell Survival; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry; D008163:Luminescent Measurements / Q000379:methods; D051379:Mice; D054118:Nanospheres / Q000737:chemistry / Q000648:ultrastructure; D010316:Particle Size; D011100:Polyglycolic Acid / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D045663:Quantum Dots; D018036:Selenium Compounds / Q000737:chemistry; D019265:Spectroscopy, Near-Infrared / Q000379:methods*; D013691:Tellurium / Q000737:chemistry; D051598:Whole Body Imaging / Q000379:methods*
D019187:Cadmium Compounds; D018036:Selenium Compounds; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; C058667:cadmium selenide; D013691:Tellurium; C028337:cadmium telluride