Protective effect of hawthorn extract against genotoxicity induced by methyl methanesulfonate in human lymphocytes.
The preventive effect of hawthorn (Crataegus microphylla) fruit extract against genotoxicity induced by methyl methanesulfonate (MMS) has been investigated in human cultured blood lymphocytes. Peripheral blood samples were collected from human volunteers at 0 (10 minutes before), and at 1 and 2 hours after a single oral ingestion of 1 g hawthorn powder extract. At each time point, the whole blood was treated in vitro with MMS (200 µmol) at 24 hours after cell culture, and then the lymphocytes were cultured with mitogenic stimulation to determine the micronuclei in cytokinesis-blocked binucleated cells. The lymphocytes treated with hawthorn and MMS to exhibit a significant decreasing in the incidence of micronucleated binucleated cells, as compared with similarly MMS-treated lymphocytes from blood samples collected at 0 hour. The maximum protection and decreasing in frequency of micronuclei (36%) was observed at 1 hour after ingestion of hawthorn extract. The high performance liquid chromatography (HPLC) analysis showed that hawthorn contained chlorogenic acid, epicatechin and hyperoside. It is obvious that hawthorn, particularly flavonoids constituents with antioxidative activity, reduced the oxidative stress and genotoxicity induced by toxic compounds. This set of data may have an important application for the protection of human lymphocyte from the genetic damage and side effects induced by chemicals hazardous in people.
Hosseinimehr
Seyed Jalal
SJ
Department of Radiopharmacy, Faculty of Pharmacy, Traditional and Complementary Medicine Research Center, Mazandaran University of Medical Sciences, Sari, Iran. sjhosseinim@mazums.ac.ir
Azadbakht
Mohammad
M
Tanha
Mohammad
M
Mahmodzadeh
Aziz
A
Mohammadifar
Sohila
S
D000328:Adult; D016587:Antimutagenic Agents / Q000494:pharmacology*; D002392:Catechin / Q000032:analysis; D002478:Cells, Cultured; D002726:Chlorogenic Acid / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D027825:Crataegus / Q000737:chemistry*; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects*; D008297:Male; D008741:Methyl Methanesulfonate / Q000633:toxicity*; D048629:Micronuclei, Chromosome-Defective / Q000187:drug effects; D015162:Micronucleus Tests; D009153:Mutagens / Q000633:toxicity*; D010936:Plant Extracts / Q000032:analysis / Q000494:pharmacology*; D011794:Quercetin / Q000031:analogs & derivatives / Q000032:analysis; D013997:Time Factors
D016587:Antimutagenic Agents; D009153:Mutagens; D010936:Plant Extracts; D002726:Chlorogenic Acid; C021304:hyperoside; D002392:Catechin; D011794:Quercetin; D008741:Methyl Methanesulfonate