Peptide fractionation in proteomics approaches.
Peptide fractionation is extremely important in proteomics approaches. Full proteome characterization is desired from complex organisms, and with growing interest in post-translational modifications an extended protein sequence coverage is required. Peptide fractionation techniques have the great challenge of feeding current mass spectrometers in a way in which these issues are met. Peptide fractionation can be divided into three simple components: the column characteristics; the mobile phase; and peptide properties (charge, polarity, hydrophobicity and size). The current challenges are in the combination of these three components to allow comprehensive proteomics studies to be improved.
Manadas
Bruno
B
Proteomics Unit, Center for Neuroscience and Cell Biology, University of Coimbra, Biocant - Parque Tecnológico de Cantanhede, Núcleo 04, Lote 3, 3060-197 Cantanhede, Portugal. bmanadas@cnc.cj.uc.pt
Mendes
Vera M
VM
English
Jane
J
Dunn
Michael J
MJ
D002852:Chromatography, Ion Exchange; D002853:Chromatography, Liquid; D057927:Hydrophobic and Hydrophilic Interactions; D007525:Isoelectric Focusing / Q000379:methods; D010455:Peptides / Q000737:chemistry*; D040901:Proteomics / Q000379:methods*
D010455:Peptides