Urinary 8-hydroxy-2'-deoxyguanosine reflects symptomatic status and severity of systolic dysfunction in patients with chronic heart failure.
OBJECTIVE
Oxidative stress is known to play a crucial role in the pathogenesis of heart failure (HF). We investigated whether urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG), a product of oxidative DNA damage, is a clinically useful biomarker of the severity of chronic heart failure (CHF) and oxidative stress levels in failing hearts.


RESULTS
We measured 8-OHdG in the serum obtained from the coronary sinus (CS) and aortic root (Ao) in small groups of control subjects and CHF patients. We then measured urinary 8-OHdG and other biomarkers (brain natriuretic peptide, 8-isoplastane, high-sensitivity C-reactive protein, interleukin-6, and tumour necrosis factor-α) in 31 control subjects and 194 patients with CHF [left-ventricular ejection fraction (LVEF): 28.3 ± 8.1%]. Serum 8-OHdG was significantly higher in the CS than the Ao in CHF patients only. Urinary 8-OHdG was also significantly higher in CHF patients than in control subjects, and urinary 8-OHdG became higher as New York Heart Association class increased. Moreover, there was a significant correlation between urinary 8-OHdG and LVEF (r = -0.27), pulmonary capillary wedge pressure (r = 0.31), or left-ventricular end-diastolic volume index (r = 0.22). In contrast, there was poor correlation between the severity of CHF and the other neurohumoral biomarkers.


CONCLUSIONS
In HF, urinary 8-OHdG seems to reflect the level of oxidative stress and various parameters related to symptomatic status and functional severity of CHF.
Kobayashi
Shigeki
S
Department of Medicine and Clinical Science, Yamaguchi University Graduate School of Medicine, 1-1-1 Minamikogushi, Ube, Yamaguchi 755-8505, Japan. skoba@yamaguchi-u.ac.jp
Susa
Takehisa
T
Tanaka
Takeo
T
Wada
Yasuaki
Y
Okuda
Shinichi
S
Doi
Masahiro
M
Nao
Tomoko
T
Yoshiga
Yasuhiro
Y
Yamada
Jutaro
J
Okamura
Takayuki
T
Ueyama
Takeshi
T
Kawamura
Syuji
S
Yano
Masafumi
M
Matsuzaki
Masunori
M
D000080242:8-Hydroxy-2'-Deoxyguanosine; D001011:Aorta; D015415:Biomarkers / Q000097:blood / Q000652:urine; D016022:Case-Control Studies; D054326:Coronary Sinus; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000097:blood / Q000652:urine; D018450:Disease Progression; D005260:Female; D054143:Heart Failure, Systolic / Q000097:blood / Q000473:pathology / Q000652:urine*; D006801:Humans; D016014:Linear Models; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D018384:Oxidative Stress / Q000502:physiology; D011379:Prognosis; D017382:Reactive Oxygen Species; D012720:Severity of Illness Index; D013318:Stroke Volume; D016277:Ventricular Function, Left
D015415:Biomarkers; D017382:Reactive Oxygen Species; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine