A bacterial laccase from marine microbial metagenome exhibiting chloride tolerance and dye decolorization ability.
Laccases are blue multicopper oxidases with potential applications in environmental and industrial biotechnology. In this study, a new bacterial laccase gene of 1.32 kb was obtained from a marine microbial metagenome of the South China Sea by using a sequence screening strategy. The protein (named as Lac15) of 439 amino acids encoded by the gene contains three conserved Cu(2+)-binding domains, but shares less than 40% of sequence identities with all of the bacterial multicopper oxidases characterized. Lac15, recombinantly expressed in Escherichia coli, showed high activity towards syringaldazine at pH 6.5-9.0 with an optimum pH of 7.5 and with the highest activity occurring at 45 °C. Lac15 was stable at pH ranging from 5.5 to 9.0 and at temperatures from 15 to 45 °C. Distinguished from fungal laccases, the activity of Lac15 was enhanced twofold by chloride at concentrations lower than 700 mM, and kept the original level even at 1,000 mM chloride. Furthermore, Lac15 showed an ability to decolorize several industrial dyes of reactive azo class under alkalescent conditions. The properties of alkalescence-dependent activity, high chloride tolerance, and dye decolorization ability make the new laccase Lac15 an alternative for specific industrial applications.
Fang
Zemin
Z
School of Life Sciences & Biotechnology Center, Anhui University, Hefei, Anhui 230039, China.
Li
Tongliang
T
Wang
Quan
Q
Zhang
Xuecheng
X
Peng
Hui
H
Fang
Wei
W
Hong
Yuzhi
Y
Ge
Honghua
H
Xiao
Yazhong
Y
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D001391:Azo Compounds / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D002681:China; D002712:Chlorides / Q000378:metabolism*; D003001:Cloning, Molecular; D004396:Coloring Agents / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000378:metabolism*; D004795:Enzyme Stability; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D006835:Hydrazones / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D042845:Laccase / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D054892:Metagenome; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity; D013696:Temperature; D014871:Water Microbiology
D001391:Azo Compounds; D001426:Bacterial Proteins; D002712:Chlorides; D004396:Coloring Agents; D004791:Enzyme Inhibitors; D006835:Hydrazones; C009370:syringaldazine; D042845:Laccase