Measurement of the vitamin E metabolites, carboxyethyl hydroxychromans (CEHCs), in biological samples.
Metabolites of α- and γ-tocopherol, 2,5,7,8-tetramethyl-2-(2'-carboxyethyl)-6-hydroxychroman (α-CEHC) and 2,7,8-trimethyl-2-(β-carboxyethyl)-6-hydroxychroman (γ-CEHC), respectively, are produced in the liver and have been measured in biological fluids and tissue. Compared to α-tocopherol concentrations, metabolite concentrations are as much as a factor of a thousand lower, requiring extremely sensitive methodology to attain accurate measurements. This unit presents a protocol for CEHC extraction from biological samples, and describes very specific and sensitive HPLC/MS analysis.
Leonard
Scott W
SW
Linus Pauling Institute, Oregon State University, Corvallis, Oregon, USA.
Traber
Maret G
MG
D000818:Animals; D002839:Chromans / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D013058:Mass Spectrometry; D011422:Propionates / Q000032:analysis*; D014810:Vitamin E / Q000378:metabolism*
D002839:Chromans; D011422:Propionates; D014810:Vitamin E; C107690:2,7,8-trimethyl-2-(beta-carboxyethyl)-6-hydroxychroman; C084852:2,5,7,8-tetramethyl-2-(2'-carboxyethyl)-6-hydroxychroman