Improved capacity of a monkey-tropic HIV-1 derivative to replicate in cynomolgus monkeys with minimal modifications.
Human immunodeficiency virus type 1 (HIV-1) hardly replicates in Old World monkeys. Recently, a mutant HIV-1 clone, NL-DT5R, in which a small part of gag and the entire vif gene are replaced with SIVmac239-derived ones, was shown to be able to replicate in pigtail monkeys but not in rhesus monkeys (RM). In the present study, we found that a modified monkey-tropic HIV-1 (HIV-1mt), MN4-5S, acquired the ability to replicate efficiently in cynomolgus monkeys as compared with the NL-DT5R, while neither NL-DT5R nor MN4-5S replicated in RM cells. These results suggest that multiple determinants may be involved in the restriction of HIV-1 replication in macaques, depending on the species of macaques. The new HIV-1mt clone will be useful for studying molecular mechanisms by which anti-viral host factors regulate HIV-1 replication in macaques.
Saito
Akatsuki
A
International Research Center for Infectious Diseases, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan.
Nomaguchi
Masako
M
Iijima
Sayuki
S
Kuroishi
Ayumu
A
Yoshida
Tomoyuki
T
Lee
Young-Jung
YJ
Hayakawa
Toshiyuki
T
Kono
Ken
K
Nakayama
Emi E
EE
Shioda
Tatsuo
T
Yasutomi
Yasuhiro
Y
Adachi
Akio
A
Matano
Tetsuro
T
Akari
Hirofumi
H
D000818:Animals; D015658:HIV Infections; D015497:HIV-1 / Q000235:genetics*; D058507:Host Specificity / Q000502:physiology*; D006801:Humans; D008212:Lymphocyte Depletion; D008252:Macaca fascicularis / Q000821:virology*; D015302:Simian Immunodeficiency Virus / Q000235:genetics / Q000502:physiology; D014766:Viremia; D014779:Virus Replication