Peptide fractionation in proteomics approaches.
Peptide fractionation is extremely important in proteomics approaches. Full proteome characterization is desired from complex organisms, and with growing interest in post-translational modifications an extended protein sequence coverage is required. Peptide fractionation techniques have the great challenge of feeding current mass spectrometers in a way in which these issues are met. Peptide fractionation can be divided into three simple components: the column characteristics; the mobile phase; and peptide properties (charge, polarity, hydrophobicity and size). The current challenges are in the combination of these three components to allow comprehensive proteomics studies to be improved.
Proteomics Unit, Center for Neuroscience and Cell Biology, University of Coimbra, Biocant - Parque Tecnológico de Cantanhede, Núcleo 04, Lote 3, 3060-197 Cantanhede, Portugal. bmanadas@cnc.cj.uc.pt
Bruno
B
Manadas
Vera M
VM
Mendes
Jane
J
English
Michael J
MJ
Dunn
D002852:Chromatography, Ion Exchange; D002853:Chromatography, Liquid; D057927:Hydrophobic and Hydrophilic Interactions; D007525:Isoelectric Focusing / Q000379:methods; D010455:Peptides / Q000737:chemistry*; D040901:Proteomics / Q000379:methods*
D010455:Peptides