Transplantation of photoreceptor and total neural retina preserves cone function in P23H rhodopsin transgenic rat.
BACKGROUND
Transplantation as a therapeutic strategy for inherited retinal degeneration has been historically viewed to restore vision as a method by replacing the lost retinal cells and attempting to reconstruct the neural circuitry with stem cells, progenitor cells and mature neural retinal cells.


RESULTS
We present evidence for an alternative strategy aimed at preventing the secondary loss of cones, the most crucial photoreceptors for vision, by transplanting normal photoreceptors cells into the eye of the P23H rat, a model of dominant retinitis pigmentosa. We carried out transplantation of photoreceptors or total neural retina in 3-month-old P23H rats and evaluated the function and cell counts 6 months after surgery. In both groups, cone loss was significantly reduced (10%) in the transplanted eyes where the cone outer segments were found to be considerably longer. This morphological effect correlated with maintenance of the visual function of cones as scored by photopic ERG recording, but more precisely with an increase in the photopic b-wave amplitudes by 100% and 78% for photoreceptor transplantation and whole retinal transplantation respectively.


CONCLUSIONS
We demonstrate here that the transplanted tissue prevents the loss of cone function, which is further translated into cone survival.
INSERM, U968, Paris, France.
Ying
Y
Yang
Saddek
S
Mohand-Said
Thierry
T
Léveillard
Valérie
V
Fontaine
Manuel
M
Simonutti
José-Alain
JA
Sahel
D000818:Animals; D020419:Photoreceptor Cells, Vertebrate / Q000637:transplantation*; D051381:Rats; D055647:Rats, Transgenic; D017949:Retinal Cone Photoreceptor Cells / Q000502:physiology*; D012243:Rhodopsin / Q000235:genetics / Q000502:physiology*
D012243:Rhodopsin