Restricted feeding improves postischemic recovery of Langendorff-perfused rat hearts.
The goal of the present study was to assess the effects of a restricted feeding schedule (RFS) on postischemic contractile recovery in relation to triacylglycerol (TAG), glycogen, and ATP content. Glucose-6-phosphate dehydrogenase (G6PDH) activity, reduced/oxidized glutathione ratio (GSH/GSSG), and thiobarbituric acid reactive substances (TBARS) levels were also determined. Isolated rat hearts entrained to daily RFS (2 h food access starting at 1200) or fed ad libitum (FED) for 3 weeks were Langendorff-perfused (25 min ischemia, 30 min reperfusion) with Krebs-Ringer bicarbonate solution (10 mmol/L glucose). RFS improved the recovery of contractility and reduced creatine kinase (CK) release upon reperfusion. Further, at the end of reperfusion, RFS hearts exhibited increased G6PDH activity and repletion of tissue glycogen, TAG, and ATP that was not observed in the FED hearts. GSH/GSSG at the end of reperfusion fell to the same value in both nutritional states, and TBARS levels were higher in the RFS hearts. In conclusion, RFS improved postischemic functional recovery, which was accompanied by a reduction in CK release and a striking energy recovery. Although enhanced G6PDH activity was displayed, RFS was unable to reduce lipid peroxidation, supporting a clear dissociation between protection against mechanical dysfunction and CK release on the one hand and oxidative damage on the other.
Department of Biological Sciences, University of Buenos Aires, IQUIMEFA-CONICET, Buenos Aires, Argentina.
María M
MM
Jaitovich
Alicia
A
Varela
Cintia
C
Sbarbati
Romina
R
Hermann
María E
ME
Torresín
Enrique A
EA
Savino
María G
MG
Marina Prendes
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D031204:Caloric Restriction; D003402:Creatine Kinase / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D006003:Glycogen / Q000378:metabolism; D006334:Heart Function Tests; D006352:Heart Ventricles / Q000378:metabolism / Q000503:physiopathology; D015227:Lipid Peroxidation / Q000502:physiology; D009200:Myocardial Contraction / Q000502:physiology; D017202:Myocardial Ischemia / Q000503:physiopathology / Q000517:prevention & control*; D010477:Perfusion; D051381:Rats; D017208:Rats, Wistar; D014280:Triglycerides / Q000378:metabolism; D016277:Ventricular Function, Left / Q000502:physiology
D014280:Triglycerides; D000255:Adenosine Triphosphate; D006003:Glycogen; D005954:Glucosephosphate Dehydrogenase; D003402:Creatine Kinase; D005978:Glutathione