Characterization of pseudo-response regulators in plants.
A small family of clock-regulated pseudo-response regulators (PRRs) plays a number of critical roles in the function of the plant circadian clock. The regulation of the PRRs is complex and entails both transcriptional and posttranslational regulation. PRR proteins engage in a number of important protein-protein interactions, some of which are modulated by modifications including phosphorylation. PRR stability is also tightly controlled. This chapter provides methods for studying both the PRR genes and their encoded proteins.
Department of Plant Cellular and Molecular Biology, Ohio State University, Columbus, Ohio, USA.
Woe-Yeon
WY
Kim
Patrice A
PA
Salomé
Sumire
S
Fujiwara
David E
DE
Somers
C Robertson
CR
McClung
D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002940:Circadian Rhythm / Q000235:genetics / Q000502:physiology; D018506:Gene Expression Regulation, Plant / Q000235:genetics / Q000502:physiology; D047468:Immunoprecipitation; D010766:Phosphorylation / Q000235:genetics / Q000502:physiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D012097:Repressor Proteins; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
D029681:Arabidopsis Proteins; C523307:PRR5 protein, Arabidopsis; C523308:PRR7 protein, Arabidopsis; C514751:PRR9 protein, Arabidopsis; D010940:Plant Proteins; D012097:Repressor Proteins; C411822:TOC1 protein, Arabidopsis; D014157:Transcription Factors