Tissue response and biodegradation of composite scaffolds prepared from Thai silk fibroin, gelatin and hydroxyapatite.
This work aimed to investigate tissue responses and biodegradation, both in vitro and in vivo, of four types of Bombyx mori Thai silk fibroin based-scaffolds. Thai silk fibroin (SF), conjugated gelatin/Thai silk fibroin (CGSF), hydroxyapatite/Thai silk fibroin (SF4), and hydroxyapatite/conjugated gelatin/Thai silk fibroin (CGSF4) scaffolds were fabricated using salt-porogen leaching, dehydrothermal/chemical crosslinking and an alternate soaking technique for mineralization. In vitro biodegradation in collagenase showed that CGSF scaffolds had the slowest biodegradability, due to the double crosslinking by dehydrothermal and chemical treatments. The hydroxyapatite deposited from alternate soaking separated from the surface of the protein scaffolds when immersed in collagenase. From in vivo biodegradation studies, all scaffolds could still be observed after 12 weeks of implantation in subcutaneous tissue of Wistar rats and also following ISO10993-6: Biological evaluation of medical devices. At 2 and 4 weeks of implantation the four types of Thai silk fibroin based-scaffolds were classified as "non-irritant" to "slight-irritant", compared to Gelfoam(®) (control samples). These natural Thai silk fibroin-based scaffolds may provide suitable biomaterials for clinical applications.
Biomedical Program, Graduate School, Chulalongkorn University, Phayathai Road, Bangkok, 10330, Thailand.
Hathairat
H
Tungtasana
Somruetai
S
Shuangshoti
Shanop
S
Shuangshoti
Sorada
S
Kanokpanont
David L
DL
Kaplan
Tanom
T
Bunaprasert
Siriporn
S
Damrongsakkul
D020341:Absorbable Implants / Q000009:adverse effects; D000818:Animals; D003188:Composite Resins / Q000138:chemical synthesis / Q000493:pharmacokinetics* / Q000494:pharmacology*; D017886:Durapatite / Q000737:chemistry* / Q000493:pharmacokinetics / Q000494:pharmacology; D005260:Female; D005349:Fibroins / Q000737:chemistry* / Q000493:pharmacokinetics / Q000494:pharmacology; D005780:Gelatin / Q000737:chemistry* / Q000493:pharmacokinetics / Q000494:pharmacology; D008422:Materials Testing; D019919:Prosthesis Implantation / Q000379:methods; D051381:Rats; D017208:Rats, Wistar; D047011:Silk; D040521:Subcutaneous Tissue / Q000187:drug effects / Q000378:metabolism; D054457:Tissue Scaffolds / Q000009:adverse effects / Q000737:chemistry
D003188:Composite Resins; D047011:Silk; D005780:Gelatin; D005349:Fibroins; D017886:Durapatite