Response of astrocytes and microglia/macrophages to brain injury after bone marrow stromal cell transplantation: a quantitative study.
The aim of the present study was to investigate the effects of intracerebral and intravenous administration of bone marrow stromal cells (BMSCs) on cellular activity in the injured brain. Female Wistar rats were subjected to cerebral cortex injury followed by the injection of BMSCs or saline, directly to the injured site or to the tail vein. Lectin histochemistry and glial fibrillary acidic protein immunohistochemistry were used to analyze the number of microglia/macrophages and astrocytes in the injured cerebral cortex, respectively. BMSC treatment affected cell response to brain injury. The effects of BMSC action were dependent on the site of their administration. The intravenous injection of BMSCs noticeably increased the number of microglia/macrophages in the injured brain. Intracerebral transplantation of BMSCs significantly enhanced the number of astrocytes, and in a less degree caused changes in the number of microglia/macrophages. The results suggest that BMSCs can affect the restorative processes in the injured brain through stimulation of the cellular response to injury.
Department of Experimental Hematology, Jagiellonian University, R. Ingardena 6, 30-060 Cracow, Poland. malgorzata.opydo-chanek@uj.edu.pl
Małgorzata
M
Opydo-Chanek
Zbigniew
Z
Dąbrowski
D000818:Animals; D001253:Astrocytes / Q000473:pathology*; D016026:Bone Marrow Transplantation / Q000009:adverse effects* / Q000473:pathology; D001930:Brain Injuries / Q000209:etiology / Q000473:pathology*; D002452:Cell Count / Q000379:methods; D002465:Cell Movement / Q000502:physiology; D002478:Cells, Cultured; D005260:Female; D008264:Macrophages / Q000473:pathology*; D017628:Microglia / Q000473:pathology*; D011183:Postoperative Complications / Q000209:etiology / Q000473:pathology*; D051381:Rats; D017208:Rats, Wistar; D017154:Stromal Cells / Q000473:pathology / Q000637:transplantation