Measurement of uroporphyrinogen decarboxylase activity.
Uroporphyrinogen decarboxylase (UROD) catalyzes decarboxylation of the four acetate side chains of urophyrinogen to form coproporphyrinogen. Activity of UROD can be measured using an enzymatically prepared substrate or a chemically prepared one. For the former, bacterial porphobilinogen deaminase is prepared and used to prepare the porphyrinogen substrate for the enzymatic assay. Erythrocyte lysates can be used to measure hemoglobin content as an indicator of UROD activity.
University of Utah Medical School, Salt Lake City, Utah, USA.
J D
JD
Phillips
J P
JP
Kushner
D000818:Animals; D001426:Bacterial Proteins / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D004912:Erythrocytes / Q000201:enzymology; D006801:Humans; D011163:Hydroxymethylbilane Synthase / Q000302:isolation & purification; D012242:Rhodobacter sphaeroides / Q000201:enzymology; D014575:Uroporphyrinogen Decarboxylase / Q000032:analysis*
D001426:Bacterial Proteins; D011163:Hydroxymethylbilane Synthase; D014575:Uroporphyrinogen Decarboxylase