Calcitonin gene-related peptide- and adrenomedullin-induced facilitation of calcium current in submandibular ganglion.
OBJECTIVE
The control of saliva secretion is mainly under parasympathetic control. The submandibular ganglion (SMG) is a parasympathetic ganglion which receives inputs from preganglionic cholinergic neurons, and innervates the submandibular salivary gland to control saliva secretion. The aim of this study was to investigate if adrenomedullin (ADM) and/or calcitonin gene-related peptide (CGRP) modulate voltage-dependent calcium channel (VDCCs) current (I(Ca)) in SMG.


METHODS
The profile of CGRP and ADM actions in SMG was studied using the whole-cell configuration of the patch-clamp technique.


RESULTS
Both ADM and CGRP facilitated I(Ca). These facilitations were attenuated by intracellular dialysis of the anti-Gα(s)-protein and pretreatment of SQ22536 (an adenylate cyclase inhibitor).


CONCLUSIONS
ADM and CGRP facilitates VDCCs mediated by Gα(s)-protein and adenylate cyclase in SMG.
Oral Health Science Center HRC7, Tokyo Dental College, 1-2-2 Masago, Mihama-ku, Chiba 261-8502, Japan.
Takayuki
T
Endoh
Yoshiyuki
Y
Shibukawa
Maki
M
Tsumura
Hideki
H
Ichikawa
Masakazu
M
Tazaki
Takashi
T
Inoue
D000262:Adenylyl Cyclases / Q000378:metabolism; D053607:Adrenomedullin / Q000378:metabolism*; D000704:Analysis of Variance; D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism*; D015220:Calcium Channels / Q000502:physiology*; D006224:Cricetinae; D005726:Ganglia, Parasympathetic / Q000502:physiology*; D008297:Male; D008647:Mesocricetus; D009474:Neurons / Q000502:physiology*; D018408:Patch-Clamp Techniques; D012472:Salivation / Q000502:physiology*; D013363:Submandibular Gland / Q000502:physiology*
D015220:Calcium Channels; D053607:Adrenomedullin; D000262:Adenylyl Cyclases; D015740:Calcitonin Gene-Related Peptide