Characterization of voltage-activated ionic currents in the GnRH-containing terminalis nerve in transgenic zebrafish.
The terminalis nerve (TN) is in a class of cranial nerves that plays important roles in animal development, physiology and behavior. Here, we report a study on the characterization of voltage-activated ionic currents in GnRH-containing TN cells in zebrafish. The experiments were performed using acutely dissociated TN cells from the transgenic zebrafish Tg (GnRH-3::GFP). In the transgenic zebrafish, the TN cells express GFP under the transcriptional control of the zebrafish GnRH-3 promoter. In all of the GnRH-containing TN cells examined, we recorded both low-voltage-activated (LVA) and high-voltage-activated (HVA) calcium current (I(Ca)). The characteristics of the I(Ca) were similar to those described in other zebrafish cell types. However, the distribution patterns of the currents in the GnRH-containing TN cells were different in comparison to the distribution of the currents in other cell types. In addition, we characterized TTX-sensitive sodium current (I(Na)) and 4AP-sensitive and TEA-resistant potassium current (I(K)). The characteristics of voltage-activated I(Na) and I(K) in the GnRH-containing TN cells were similar to those described in other zebrafish cell types. Together, the data from this study revealed the electrophysiological properties of the GnRH-containing TN cells, thereby providing insight on the regulatory mechanisms of TN-signaling in animal physiology.
Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 46556, USA.
Luoxiu
L
Huang
Lei
L
Li
D000818:Animals; D030801:Animals, Genetically Modified; D055592:Biophysical Phenomena / Q000187:drug effects / Q000502:physiology*; D002478:Cells, Cultured; D004558:Electric Stimulation / Q000379:methods; D007987:Gonadotropin-Releasing Hormone / Q000235:genetics / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000235:genetics; D015640:Ion Channel Gating / Q000187:drug effects / Q000502:physiology; D007473:Ion Channels / Q000145:classification / Q000187:drug effects / Q000502:physiology*; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology*; D049990:Membrane Transport Modulators / Q000494:pharmacology; D009474:Neurons / Q000166:cytology*; D009830:Olfactory Bulb / Q000166:cytology; D018408:Patch-Clamp Techniques / Q000379:methods; D017729:Presynaptic Terminals / Q000502:physiology*; D015027:Zebrafish
D007473:Ion Channels; D049990:Membrane Transport Modulators; D049452:Green Fluorescent Proteins; D007987:Gonadotropin-Releasing Hormone