Cell-specific regulation of Fas exon 6 splicing mediated by Hu antigen R.
The differential expression levels of T-cell intracellular antigens (TIA) and Hu antigen R (HuR) are concomitant with a splicing switch in apoptosis receptor Fas in HCT-116 cells. Thus, overexpression and knockdown of HuR led to Fas exon 6 skipping and inclusion, respectively. These results suggest that the TIA and HuR cellular ratio influences cell-type specific Fas exon 6 splicing pattern.
Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas, Universidad Autónoma de Madrid, Lab. 107, C/Nicolás Cabrera, 1, Cantoblanco, 28049 Madrid, Spain. jmizquierdo@cbm.uam.es
José M
JM
Izquierdo
D017398:Alternative Splicing; D000954:Antigens, Surface / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D045744:Cell Line, Tumor; D051959:ELAV Proteins; D000067780:ELAV-Like Protein 1; D005091:Exons / Q000235:genetics*; D055785:Gene Knockdown Techniques; D006367:HeLa Cells; D006801:Humans; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D019014:fas Receptor / Q000235:genetics*
D000954:Antigens, Surface; D051959:ELAV Proteins; D000067780:ELAV-Like Protein 1; C098770:ELAVL1 protein, human; C491901:FAS protein, human; D016601:RNA-Binding Proteins; D019014:fas Receptor