Dispersion-based pulse shaping for multiplexed two-photon fluorescence microscopy.
We demonstrate selective two-photon excited fluorescence microscopy with shaped pulses produced with a simple yet efficient scheme based on dispersive optical components. The pulse train from a broadband oscillator is split into two subtrains that are sent through different amounts of glass. Beam recombination results in pulse-shape switching at a rate of 150MHz. Time-resolved photon counting detection then provides two simultaneous images resulting from selective two-photon excitation, as demonstrated in a live embryo. Although less versatile than programmable pulse-shaping devices, this novel arrangement significantly improves the performance of selective microscopy using broadband shaped pulses while simplifying the experimental setup.
Laboratoire d’Optique et Biosciences, Ecole Polytechnique, CNRS, and INSERM U696, 91128 Palaiseau, France.
Guillaume
G
Labroille
Rajesh S
RS
Pillai
Xavier
X
Solinas
Caroline
C
Boudoux
Nicolas
N
Olivier
Emmanuel
E
Beaurepaire
Manuel
M
Joffre