Tumor angiogenesis in keratocystic odontogenic tumor assessed by using CD-105 antigen.
BACKGROUND
The purpose of this study was to assess and compare angiogenesis with proliferative activity in Keratocystic odontogenic tumors (KCOT) and dentigerous cysts (DC) by using monoclonal mouse anti-human antibody against CD105 (endoglin).


METHODS
Angiogenesis was assessed in 38 KCOT, 27 DCs and 19 Normal Oral Mucosa (NOM) by measuring the Mean Vascular Density (MVD), Total Vascular Area (TVA) and Mean Vascular Area (MVA). Cell proliferation was assessed by obtaining Ki-67 Labeling Index (Ki-67LI) in all the groups.


RESULTS
Statistically significant difference was observed in MVD, TVA, MVA and Ki-67 LI between the KCOT, DC and NOM (P=0.000). The MVD, TVA, MVA and Ki-67 LI were significantly higher in KCOT than in DC and NOM (P=0.000). The Ki-67 LI was significantly higher in NOM than in DC (P=0.000). MVD (P=0.032) and TVA (P=0.038) were significantly higher in NOM than in DC. There was significant positive correlation between Ki-67 LI and MVD, Ki-67 and TVA and Ki-67 and MVA.


CONCLUSIONS
The result suggests that CD105 (endoglin) is strongly expressed in microvessels of KCOT compared with that in Dentigerous cyst and Normal oral mucosa. Thus, it suggests that angiogenesis may be associated with locally aggressive biological behavior of KCOT. These findings further stress on the hypothesis that the stroma of KCOT could be regarded not just as a structural support of the cyst wall, but as playing a part in the neoplastic behavior of cyst.
Department of Oral and Maxillofacial Pathology & Microbiology, Sharad Pawar Dental College & Hospital, Datta Meghe Institute of Medical Sciences, Sawangi (Meghe), Wardha, Maharashtra, India. gadbail@yahoo.co.in
Amol Ramchandra
AR
Gadbail
Alka
A
Hande
Minal
M
Chaudhary
Atul
A
Nikam
Madhuri
M
Gawande
Swati
S
Patil
Satyajit
S
Tekade
Shailesh
S
Gondivkar
D015703:Antigens, CD / Q000032:analysis*; D001485:Basement Membrane / Q000473:pathology; D049109:Cell Proliferation; D003803:Dentigerous Cyst / Q000098:blood supply; D000071063:Endoglin; D042783:Endothelial Cells / Q000473:pathology; D004730:Endothelium, Vascular / Q000473:pathology; D004848:Epithelium / Q000473:pathology; D006801:Humans; D019394:Ki-67 Antigen / Q000032:analysis; D055806:Microvessels / Q000473:pathology; D009061:Mouth Mucosa / Q000098:blood supply; D009389:Neovascularization, Pathologic / Q000473:pathology*; D018919:Neovascularization, Physiologic / Q000502:physiology; D009808:Odontogenic Tumors / Q000098:blood supply*; D011956:Receptors, Cell Surface / Q000032:analysis*
D015703:Antigens, CD; C064206:ENG protein, human; D000071063:Endoglin; D019394:Ki-67 Antigen; D011956:Receptors, Cell Surface