Micromass cultures in teratology.
This unit describes methods for culture of undifferentiated midbrain (mesencephalon) and limb bud cells from gestation day 12 rat embryos. When grown over 5 days in vitro, these mixed cell populations express many morphological, biochemical, molecular, and immunophenotypic characteristics observed during in vivo differentiation. These cultures can be used in a wide variety of studies designed to investigate normal cellular ontogeny, the teratogenic potential of test agents, or the mechanisms underlying the cellular response to environmental stress.
University of Washington, Seattle, Washington, USA.
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D000818:Animals; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology / Q000187:drug effects; D046149:Embryo Culture Techniques; D004622:Embryo, Mammalian; D004785:Environmental Pollutants / Q000633:toxicity*; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects; D005260:Female; D018878:Limb Buds / Q000166:cytology / Q000187:drug effects*; D018811:Maternal Exposure / Q000009:adverse effects; D008636:Mesencephalon / Q000166:cytology / Q000187:drug effects*; D042282:Microdissection; D051381:Rats
D004785:Environmental Pollutants