Methodological contributions towards LC-MS/MS quantification of free VX in plasma: An innovative approach.
A novel analytical method has been developed to detect and quantify VX (O-ethyl S-(2(diisopropylamino) ethyl) (methylphosphonothioate)) in plasma using an LC-MS/MS technique. VX detection and quantification in plasma following percutaneous exposure represent a formidable challenge and it is an important part of the ongoing struggle against chemical warfare agents. Liquid-liquid extraction of VX from plasma was performed and it generated a recovery rate of approximately 65% followed by an LC-MS/MS analysis in a 100% organic phase. An Allure biphenyl column (Restek) was tested with detection limit at 0.5 pg/mL (5 μL injected). Initial application was focused on human skin grafted on nude mice as an experimental model with proper adjustments done for very small quantities of plasma.
I.R.B.A., Antenne C.R.S.S.A., Service de Biospectrométrie, 24 Avenue des Maquis du Grésivaudan, BP 87-F 38702 La Tronche Cedex, France. cdebouit@hotmail.fr
Charlotte
C
Debouit
Alexandre
A
Bazire
Guy
G
Lallement
Denis
D
Daveloose
D002619:Chemical Warfare Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D009946:Organothiophosphorus Compounds / Q000097:blood*; D053719:Tandem Mass Spectrometry / Q000379:methods*
D002619:Chemical Warfare Agents; D009946:Organothiophosphorus Compounds; C009680:VX-agent