Histomorphometric analysis of different latency periods effect on new bone obtained by periosteal distraction: an experimental study in the rabbit model.
OBJECTIVE
The aim of this study was to evaluate the effects of latency period on the bone formation after periosteal distraction. For this purpose, a rabbit model was developed and histologic and histomorphometric analyses were conducted.


METHODS
Periosteal distractors were custom designed and built from stainless steel. Rabbits were divided into 2 groups of 18 each according to the duration of latency period. In group 1, the latency period was 7 days, and in group 2 it was 1 day. Distraction was performed by activating the distractor 0.25 mm twice per day. A periosteal distraction of 7.0 mm was achieved after a distraction period of 10 days. Both groups were divided to 3 subgroups according to the rabbits being killed on the 15th, 30th, or 60th day of the consolidation period. Histologic and histomorphometric analyses were performed to evaluate the bone formation.


RESULTS
In the histologic evaluation, new bone formation was observed on the lateral side of the mandible of all the rabbits. Histomorphometric measurements revealed that the mean area of newly formed bone formation was 2.62 cm(2) in group 1 and 3.26 cm(2) in group 2.


CONCLUSIONS
Periosteal distraction osteogenesis is a viable method in acquired and congenital alveolar ridge defects, resulting in new bone formation. Newly formed bone can be obtained by periosteal distraction osteogenesis applying different latency periods.
Altuğ
Hasan Ayberk
HA
Department of Oral and Maxillofacial Surgery, Gulhane Military Medical Academy, Ankara, Turkey. aybork@yahoo.com
Aydintuğ
Yavuz Sinan
YS
Sençimen
Metin
M
Günhan
Omer
O
Ortakoğlu
Kerim
K
Bayar
Gürkan Raşit
GR
Doğan
Necdet
N
D000273:Adipose Tissue; D000540:Alveolar Ridge Augmentation / Q000295:instrumentation / Q000379:methods*; D000818:Animals; D001861:Bone Regeneration; D008334:Mandible / Q000166:cytology / Q000601:surgery*; D010011:Osteocytes; D019857:Osteogenesis, Distraction / Q000295:instrumentation / Q000379:methods*; D010521:Periosteum / Q000601:surgery*; D011817:Rabbits; D013997:Time Factors