New functional sulfide oxidase-oxygen reductase supercomplex in the membrane of the hyperthermophilic bacterium Aquifex aeolicus.
Aquifex aeolicus, a hyperthermophilic and microaerophilic bacterium, obtains energy for growth from inorganic compounds alone. It was previously proposed that one of the respiratory pathways in this organism consists of the electron transfer from hydrogen sulfide (H(2)S) to molecular oxygen. H(2)S is oxidized by the sulfide quinone reductase, a membrane-bound flavoenzyme, which reduces the quinone pool. We have purified and characterized a novel membrane-bound multienzyme supercomplex that brings together all the molecular components involved in this bioenergetic chain. Our results indicate that this purified structure consists of one dimeric bc(1) complex (complex III), one cytochrome c oxidase (complex IV), and one or two sulfide quinone reductases as well as traces of the monoheme cytochrome c(555) and quinone molecules. In addition, this work strongly suggests that the cytochrome c oxidase in the supercomplex is a ba(3)-type enzyme. The supercomplex has a molecular mass of about 350 kDa and is enzymatically functional, reducing O(2) in the presence of the electron donor, H(2)S. This is the first demonstration of the existence of such a respirasome carrying a sulfide oxidase-oxygen reductase activity. Moreover, the kinetic properties of the sulfide quinone reductase change slightly when integrated in the supercomplex, compared with the free enzyme. We previously purified a complete respirasome involved in hydrogen oxidation and sulfur reduction from Aquifex aeolicus. Thus, two different bioenergetic pathways (sulfur reduction and sulfur oxidation) are organized in this bacterium as supramolecular structures in the membrane. A model for the energetic sulfur metabolism of Aquifex aeolicus is proposed.
Prunetti
Laurence
L
Laboratoire de Bioénergétique et Ingénierie des Protéines, UPR 9036, IMM, IFR88-CNRS, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France.
Infossi
Pascale
P
Brugna
Myriam
M
Ebel
Christine
C
Giudici-Orticoni
Marie-Thérèse
MT
Guiral
Marianne
M
D001419:Bacteria / Q000378:metabolism*; D001426:Bacterial Proteins / Q000737:chemistry; D004578:Electron Spin Resonance Spectroscopy; D004579:Electron Transport; D003576:Electron Transport Complex IV / Q000737:chemistry; D006859:Hydrogen / Q000737:chemistry; D006862:Hydrogen Sulfide / Q000737:chemistry; D010084:Oxidation-Reduction; D050862:Oxidoreductases Acting on Sulfur Group Donors / Q000737:chemistry*; D010100:Oxygen / Q000737:chemistry*; D010101:Oxygen Consumption; D013050:Spectrometry, Fluorescence / Q000379:methods; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
D001426:Bacterial Proteins; D006859:Hydrogen; D050862:Oxidoreductases Acting on Sulfur Group Donors; C080499:sulfide oxidase; D003576:Electron Transport Complex IV; D010100:Oxygen; D006862:Hydrogen Sulfide