Proteome analysis of bronchoalveolar lavage fluid in lung fibrosis associated with systemic sclerosis.
BACKGROUND
Interstitial lung disease (ILD) is the major cause of mortality in collagen vascular diseases. However, its pathogenesis still needs to be elucidated.


METHODS
To evaluate the alteration of certain proteins in bronchoalveolar lavage fluid (BALF) and clarify the causative role in the processes of ILD in systemic sclerosis (SSc), we compared a BALF protein profile between 5 patients with systemic sclerosis with pulmonary fibrosis (SSc-fib+) and 4 patients with systemic sclerosis without pulmonary fibrosis (SSc-fib-) using two-dimensional gel electrophoresis (2-DE), and matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS).


RESULTS
We analyzed spots over the range of 10.1kDa to 207.4kDa. SSc-fib+ patients showed increased 3 proteins compared to SSc-fib- including α2-macroglobulin, α1-antitrypsin, and pulmonary surfactant protein A and decreased 2 proteins including α2 heat shock protein (HSP) and glutathione S-transferase (GST) compared to SSc-fib- patients.


CONCLUSIONS
In conclusion, we identified several interesting proteins that might have roles in ILD of SSc patients. Further studies are warranted to clarify the role of these proteins in the processes of pulmonary fibrosis in SSc.
Department of Integrated Pulmonology, Tokyo Medical and Dental University, Japan.
Ryutaro
R
Shirahama
Yasunari
Y
Miyazaki
Tsukasa
T
Okamoto
Naohiko
N
Inase
Yasuyuki
Y
Yoshizawa
D000368:Aged; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry*; D005260:Female; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D054989:Nephrogenic Fibrosing Dermopathy / Q000150:complications / Q000235:genetics / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism; D011658:Pulmonary Fibrosis / Q000150:complications / Q000235:genetics / Q000378:metabolism*; D037662:Pulmonary Surfactant-Associated Protein A / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D000515:alpha 1-Antitrypsin / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D000511:alpha-Macroglobulins / Q000032:analysis / Q000235:genetics / Q000378:metabolism
D006360:Heat-Shock Proteins; D020543:Proteome; D037662:Pulmonary Surfactant-Associated Protein A; D000515:alpha 1-Antitrypsin; D000511:alpha-Macroglobulins; D005982:Glutathione Transferase