Influenza A virus replication is inhibited in IFN-λ2 and IFN-λ3 transfected or stimulated cells.
Interferons lambda (IFN-λ) are the most recently defined members of the class III cytokine family. To investigate whether IFN-λ2 and IFN-λ3 displayed antiviral activity against influenza A virus (IAV), a number of cell lines induced with IFNs - as well as two established cell lines (A549-IFN-λ2 and A549-IFN-λ3) - were infected with IAV. Our results indicate that IFN-λ2 has statistically significant antiviral activity in A549-IFN-λ2 (P=0.0028) although less so than IFN-λ3, which reduced viral titer to 10% (P<0.0001). The reverse was observed for cells treated with IFNs, with IFN-λ2-treated A549 cells inhibiting IAV infection more efficiently than IFN-λ3-treated A549 cells. The antiviral effect on IFN-stimulated cells was most apparent on Vero cells (compared with MDCK and HeLa). Both IFNs significantly inhibited IAV replication and inhibition was observed in a dose-dependent manner, with an optimal IFN concentration of 20 ng/ml. IFN-λ2 was more potent than IFN-λ3 on Vero cells while IFN-λ3 appeared more efficient than IFN-λ2 on MDCK and HeLa cells.
Institute of Virology, Slovak Academy of Sciences, Bratislava, Slovak Republic.
Darina
D
Svetlikova
Peter
P
Kabat
Anna
A
Ohradanova
Jaromir
J
Pastorek
Tatiana
T
Betakova
D000818:Animals; D000998:Antiviral Agents / Q000276:immunology* / Q000494:pharmacology*; D002461:Cell Line, Transformed; D045744:Cell Line, Tumor; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D006801:Humans; D009980:Influenza A virus / Q000502:physiology*; D007251:Influenza, Human / Q000188:drug therapy* / Q000276:immunology* / Q000821:virology; D007372:Interferons; D007378:Interleukins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology* / Q000494:pharmacology*; D014162:Transfection; D014779:Virus Replication / Q000187:drug effects / Q000276:immunology; D000095022:Interferon Lambda
D000998:Antiviral Agents; D007372:Interferons; D007378:Interleukins; C000624353:interferon-lambda, human; D000095022:Interferon Lambda