The lack of ADAM17 activity during embryonic development causes hemorrhage and impairs vessel formation.
BACKGROUND
ADAM17/TACE activity is important during embryonic development. We wished to investigate possible roles of this metalloprotease, focusing on vascular development.


RESULTS
Mice mutant in the enzymatic activity of ADAM17 were examined at various stages of embryonic development for vascular pattern and integrity using markers for vessel wall cells. We observed hemorrhage and edema starting at embryonic day E14.5 and becoming more severe as development proceeded; prior to embryonic day E14.5, embryos appeared normal. Staining for PECAM-1/CD31 revealed abnormalities in the patterns of branching of the embryonic vasculature at E14.5.


CONCLUSIONS
These abnormalities preceded association of pericytes or monocyte/macrophage cells with the affected vessels and, therefore, presumably arise from defects in endothelial function consequent upon failure of ADAM17 to cleave one or more substrates involved in vascular development, such as Notch, Delta, VEGFR2 or JAM-A. Our study demonstrates a role for ADAM17 in modulating embryonic vessel development and function.
Canault
Matthias
M
Immune Disease Institute, Boston, Massachusetts, United States of America.
Certel
Kaan
K
Schatzberg
Daphne
D
Wagner
Denisa D
DD
Hynes
Richard O
RO
D051722:ADAM Proteins / Q000235:genetics / Q000502:physiology*; D000072198:ADAM17 Protein; D000818:Animals; D001483:Base Sequence; D001808:Blood Vessels / Q000196:embryology*; D019521:Body Patterning; D017931:DNA Primers; D047108:Embryonic Development; D006470:Hemorrhage / Q000209:etiology*; D051379:Mice; D016133:Polymerase Chain Reaction
D017931:DNA Primers; D051722:ADAM Proteins; D000072198:ADAM17 Protein; C000606463:Adam17 protein, mouse