Microsomal biotransformation of chlorpyrifos, parathion and fenthion in rainbow trout (Oncorhynchus mykiss) and coho salmon (Oncorhynchus kisutch): mechanistic insights into interspecific differences in toxicity.
Rainbow trout often serve as a surrogate species evaluating xenobiotic toxicity in cold-water species including other salmonids of the same genus, which are listed as threatened or endangered. Biotransformation tends to show species-specific patterns that influence susceptibility to xenobiotic toxicity, particularly organophosphate insecticides (OPs). To evaluate the contribution of biotransformation in the mechanism of toxicity of three organophosphate (phosphorothionate) insecticides, (chlorpyrifos, parathion and fenthion), microsomal bioactivation and detoxification pathways were measured in gills, liver and olfactory tissues in juvenile rainbow trout (Oncorhynchus mykiss) and compared to juvenile coho salmon (Oncorhynchus kisutch). Consistent with species differences in acute toxicity, significantly higher chlorpyrifos bioactivation was found in liver microsomes of rainbow trout (up to 2-fold) when compared with coho salmon. Although bioactivation to the oxon was observed, the catalytic efficiency towards chlorpyrifos dearylation (detoxification) was significantly higher in liver for both species (1.82 and 0.79 for trout and salmon, respectively) when compared to desulfuration (bioactivation). Bioactivation of parathion to paraoxon was significantly higher (up to 2.2-fold) than detoxification to p-nitrophenol in all tissues of both species with rates of conversion in rainbow trout, again significantly higher than coho salmon. Production of fenoxon and fenthion sulfoxides from fenthion was detected only in liver and gills of both species with activities in rainbow trout significantly higher than coho salmon. NADPH-dependent cleavage of fenthion was observed in all tissues, and was the only activity detected in olfactory tissues. These results indicate rainbow trout are more sensitive than coho salmon to the acute toxicity of OP pesticides because trout have higher catalytic rates of oxon formation. Thus, rainbow trout may serve as a conservative surrogate species for the evaluation of OP pesticides in coho salmon.
Department of Environmental Sciences, University of California, Riverside, CA 92521, USA.
Ramon
R
Lavado
Daniel
D
Schlenk
D000704:Analysis of Variance; D000818:Animals; D001711:Biotransformation; D004390:Chlorpyrifos; D002851:Chromatography, High Pressure Liquid; D005284:Fenthion; D005880:Gills / Q000378:metabolism; D007306:Insecticides / Q000378:metabolism*; D008862:Microsomes, Liver / Q000378:metabolism*; D009831:Olfactory Mucosa / Q000378:metabolism; D017684:Oncorhynchus kisutch / Q000378:metabolism*; D017686:Oncorhynchus mykiss / Q000378:metabolism*; D010278:Parathion; D013045:Species Specificity; D018675:Toxicity Tests / Q000379:methods
D007306:Insecticides; D010278:Parathion; D005284:Fenthion; D004390:Chlorpyrifos