ppGpp is the major source of growth rate control in E. coli.
It is widely accepted that the DNA, RNA and protein content of Enterobacteriaceae is regulated as a function of exponential growth rates; macromolecular content increases with faster growth regardless of specific composition of the growth medium. This phenomenon, called growth rate control, primarily involves regulation of ribosomal RNA and ribosomal protein synthesis. However, it was uncertain whether the global regulator ppGpp is the major determinant for growth rate control. Therefore, here we re-evaluate the effect of ppGpp on macromolecular content for different balanced growth rates in defined media. We find that when ppGpp is absent, RNA/protein and RNA/DNA ratios are equivalent in fast and slow growing cells. Moreover, slow growing ppGpp-deficient cells with increased RNA content, display a normal ribosomal subunit composition although polysome content is reduced when compared with fast growing wild-type cells. From this we conclude that growth rate control does not occur in the absence of ppGpp. Also, artificial elevation of ppGpp or introduction of stringent RNA polymerase mutants in ppGpp-deficient cells restores this control. We believe these findings strongly argue in favour of ppGpp and against redundant regulation of growth rate control by other factors in Escherichia coli and other enteric bacteria.
Potrykus
Katarzyna
K
Laboratory of Molecular Genetics, Program in Genomics of Development, Eunice Kennedy Shriver NICHD, NIH, Bethesda, MD 20892-2785, USA.
Murphy
Helen
H
Laboratory of Molecular Genetics, Program in Genomics of Development, Eunice Kennedy Shriver NICHD, NIH, Bethesda, MD 20892-2785, USA.
Philippe
Nadège
N
Laboratory of Molecular Genetics, Program in Genomics of Development, Eunice Kennedy Shriver NICHD, NIH, Bethesda, MD 20892-2785, USA.
Cashel
Michael
M
Laboratory of Molecular Genetics, Program in Genomics of Development, Eunice Kennedy Shriver NICHD, NIH, Bethesda, MD 20892-2785, USA.
D001426:Bacterial Proteins / Q000032:analysis; D004269:DNA, Bacterial / Q000032:analysis; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000235:genetics; D006159:Guanosine Tetraphosphate / Q000378:metabolism*; D014176:Protein Biosynthesis; D012329:RNA, Bacterial / Q000032:analysis; D012335:RNA, Ribosomal / Q000737:chemistry; D012270:Ribosomes / Q000235:genetics / Q000378:metabolism
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D012329:RNA, Bacterial; D012335:RNA, Ribosomal; C090751:dksA protein, E coli; D006159:Guanosine Tetraphosphate; D012321:DNA-Directed RNA Polymerases