Imaging single mRNA molecules in yeast.
Yeast cells in an isogenic population do not all display the same phenotypes. To study such variation within a population of cells, we need to perform measurements on each individual cell instead of measurements that average out the behavior of a cell over the entire population. Here, we provide the basic concepts and a step-by-step protocol for a recently developed technique enabling one such measurement: fluorescence in situ hybridization that renders single mRNA molecule visible in individual fixed cells.
Youk
Hyun
H
Department of Physics, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
Raj
Arjun
A
van Oudenaarden
Alexander
A
D009693:Nucleic Acid Hybridization / Q000379:methods*; D012333:RNA, Messenger / Q000235:genetics*; D015003:Yeasts / Q000235:genetics*
D012333:RNA, Messenger