[Microbial lipid production by Rhodosporidium toruloides in a two-stage culture mode].
To shorten the cultivation time and reduce the consumption of raw materials for microbial lipid production, oleaginous yeast Rhodosporidium toruloides AS 2.1389 was cultivated using a two-stage culture mode, in which the cell propagation and lipid accumulation were separated. The yeast cells recovered from the propagation culture were re-suspended in glucose solution for lipid accumulation, through which lipid content over 55% of the dry cell weight was achieved, the longer the propagation stage was, the higher the lipid content. Analysis of the lipid indicated that the long-chain fatty acids with 16 and 18 carbon atoms were major components, suggesting that the lipid can be an alternative feedstock for biodiesel production.
Lin
Jintao
J
Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
Shen
Hongwei
H
Zhang
Zehui
Z
Hu
Cuimin
C
Jin
Guojie
G
Tan
Haidong
H
Zhao
Zongbao K
ZK
D001487:Basidiomycota / Q000254:growth & development* / Q000378:metabolism*; D056804:Biofuels; D018929:Cell Culture Techniques / Q000379:methods*; D005285:Fermentation; D007218:Industrial Microbiology / Q000379:methods*; D008055:Lipids / Q000096:biosynthesis*
D056804:Biofuels; D008055:Lipids