[Progress on biodiesel production with enzymatic catalysis in China].
This paper reports the progress of biodiesel production with enzymatic catalysis in Beijing University of Chemical Technology, one of the leaders in biodiesel R & D in China, which includes screening of high-yield lipase production strains, optimization and scale-up of the lipase fermentation process, lipase immobilization, bioreactor development and scale-up, biodiesel separation and purification and the by-product glycerol utilization. Firstly, lipase fermentation was carried out at industrial scale with the 5 m3 stirred tank bioreactor, and the enzyme activity as high as 8 000 IU/mL was achieved by the species Candida sp. 99-125. Then, the lipase was purified and immobilized on textile membranes. Furthermore, biodiesel production was performed in the 5 m3 stirred tank bioreactor with an enzyme dosage as low as 0.42%, and biodiesel that met the German biodiesel standard was produced. And in the meantime, the byproduct glycerol was used for the production of 1,3-propanediol to partly offset the production cost of biodiesel, and 76.1 g/L 1,3-propanediol was obtained in 30 L fermentor with the species Klebsiella pneumoniae.
Tan
Tianwei
T
Belting Bioprocess Key Laboratory, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China. twtan@mail.buct.edu.cn
Lu
Jike
J
Nie
Kaili
K
Zhang
Haixia
H
Deng
Li
L
Wang
Fang
F
D056804:Biofuels; D019149:Bioreactors; D001709:Biotechnology / Q000191:economics / Q000379:methods*; D002175:Candida / Q000201:enzymology; D002384:Catalysis; D002681:China; D004800:Enzymes, Immobilized / Q000378:metabolism*; D004951:Esterification; D005285:Fermentation; D008049:Lipase / Q000378:metabolism*; D010938:Plant Oils / Q000737:chemistry
D056804:Biofuels; D004800:Enzymes, Immobilized; D010938:Plant Oils; D008049:Lipase