Determination of benzimidazole residues in bovine milk by ultra-high performance liquid chromatography-tandem mass spectrometry.
A method based on ultra-high performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry (UHPLC-MS/MS) for the simultaneous determination of benzimidazole residues in bovine milk has been optimized and validated. Rapid chromatographic separation of 13 analytes in 8 min was obtained by means of UHPLC. The samples were subject to Oasis MCX solid-phase extraction cartridges for extraction and clean-up. Matrix-matched calibration curves were performed to compensate for the matrix effect and loss in sample preparation. Mean recoveries ranged from 80% to 101% and inter-day precision was lower than 14%. Limit of detection and limit of quantification of the method ranged from 0.01 to 0.5 μg L⁻¹ and from 0.1 to 1.0 μg L⁻¹, respectively.
Department of Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
Xi
X
Xia
Yichun
Y
Dong
Pengjie
P
Luo
Xia
X
Wang
Xiaowei
X
Li
Shuangyang
S
Ding
Jianzhong
J
Shen
D000818:Animals; D000871:Anthelmintics / Q000032:analysis*; D001562:Benzimidazoles / Q000032:analysis*; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004350:Drug Residues / Q000032:analysis*; D008892:Milk / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods*
D000871:Anthelmintics; D001562:Benzimidazoles; C031000:benzimidazole