Molecular dynamics simulations of human and dog gastric lipases: insights into domain movements.
Mammalian gastric lipases are stable and active under acidic conditions and also in the duodenal lumen. There has been considerable interest in acid stable lipases owing to their potential application in the treatment of pancreatic exocrine insufficiency. In order to gain insights into the domain movements of these enzymes, molecular dynamics simulations of human gastric lipase was performed at an acidic pH and under neutral conditions. For comparative studies, simulation of dog gastric lipase was also performed at an acidic pH. Analyses show, that in addition to the lid region, there is another region of high mobility in these lipases. The potential role of this novel region is discussed.
Centre for Biotechnology, Anna University Chennai, Chennai 600 025, India.
Anitha
A
Selvan
Chandrabhan
C
Seniya
Srinivas Niranj
SN
Chandrasekaran
Nithyanand
N
Siddharth
Sharmila
S
Anishetty
Gautam
G
Pennathur
D000595:Amino Acid Sequence; D000818:Animals; D004285:Dogs; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008049:Lipase / Q000737:chemistry* / Q000378:metabolism*; D056004:Molecular Dynamics Simulation; D008969:Molecular Sequence Data; D009068:Movement; D017434:Protein Structure, Tertiary; D051381:Rats; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D008049:Lipase; C485982:LIPF protein, human