Crucial function of histone deacetylase 1 for differentiation of teratomas in mice and humans.
Histone deacetylase (HDAC) inhibitors induce cell cycle arrest, differentiation or apoptosis in tumour cells and are, therefore, promising anti-cancer reagents. However, the specific HDAC isoforms that mediate these effects are not yet identified. To explore the role of HDAC1 in tumourigenesis and tumour proliferation, we established an experimental teratoma model using wild-type and HDAC1-deficient embryonic stem cells. HDAC1-deficient teratomas showed no significant difference in size compared with wild-type teratomas. Surprisingly, loss of HDAC1 was not only linked to increased apoptosis, but also to significantly enhanced proliferation. Epithelial structures showed reduced differentiation as monitored by Oct3/4 expression and changed E-cadherin localization and displayed up-regulated expression of SNAIL1, a regulator of epithelial cell plasticity. Increased levels of the transcriptional regulator SNAIL1 are crucial for enhanced proliferation and reduced differentiation of HDAC1-deficient teratoma. Importantly, the analysis of human teratomas revealed a similar link between loss of HDAC1 and enhanced tumour malignancy. These findings reveal a novel role for HDAC1 in the control of tumour proliferation and identify HDAC1 as potential marker for benign teratomas.
Department of Medical Biochemistry, Max F. Perutz Laboratories, Medical University of Vienna, Vienna Biocenter, Vienna, Austria.
Sabine
S
Lagger
Dominique
D
Meunier
Mario
M
Mikula
Reinhard
R
Brunmeir
Michaela
M
Schlederer
Matthias
M
Artaker
Oliver
O
Pusch
Gerda
G
Egger
Astrid
A
Hagelkruys
Wolfgang
W
Mikulits
Georg
G
Weitzer
Ernst W
EW
Muellner
Martin
M
Susani
Lukas
L
Kenner
Christian
C
Seiser
D000818:Animals; D017209:Apoptosis; D015820:Cadherins / Q000235:genetics; D018236:Carcinoma, Embryonal / Q000201:enzymology / Q000235:genetics / Q000473:pathology; D002460:Cell Line; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism* / Q000473:pathology; D015972:Gene Expression Regulation, Neoplastic; D056284:Histone Deacetylase 1 / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D051379:Mice; D050814:Octamer Transcription Factor-3 / Q000235:genetics; D010641:Phenotype; D000071250:Snail Family Transcription Factors; D013724:Teratoma / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D014157:Transcription Factors / Q000235:genetics
D015820:Cadherins; D050814:Octamer Transcription Factor-3; C000604407:SNAI1 protein, human; C000604429:Snai1 protein, mouse; D000071250:Snail Family Transcription Factors; D014157:Transcription Factors; D056284:Histone Deacetylase 1