Subcellular localization of the human papillomavirus 16 E7 oncoprotein in CaSki cells and its detection in cervical adenocarcinoma and adenocarcinoma in situ.
E7 is the major oncoprotein of high-risk human papillomaviruses (HPV) which causes cervical cancer. To date E7 oncoproteins have not been investigated in cervical adenocarcinoma. In this study we generated a rabbit monoclonal anti-HPV-16 E7 antibody, RabMab42-3, which recognizes a conformational epitope in the E7 carboxy-terminal zinc-finger resulting in a strong increase in the sensitivity for the detection of cell-associated HPV-16 E7 protein relative to conventional polyclonal anti-HPV-16 E7 antibodies. Using RabMab42-3, we show that the subcellular localization of endogenous HPV-16 E7 oncoprotein varies during the cell cycle in cervical cancer cells. Moreover, we demonstrate for the first time that the HPV-16 E7 oncoprotein is abundantly expressed in cervical adenocarcinoma in situ and adenocarcinoma, suggesting an important role of HPV-16 E7 for the development of these tumors. Our findings suggest that the HPV-16 E7 oncoprotein could be a useful marker for the detection of cervical adenocarcinoma and their precursors.
Dreier
Kerstin
K
Institute for Biomedical Aging Research of the Austrian Academy of Sciences, Innsbruck, Austria.
Scheiden
René
R
Lener
Barbara
B
Ehehalt
Daniela
D
Pircher
Haymo
H
Müller-Holzner
Elisabeth
E
Rostek
Ursula
U
Kaiser
Andreas
A
Fiedler
Marc
M
Ressler
Sigrun
S
Lechner
Stefan
S
Widschwendter
Andreas
A
Even
Jos
J
Capesius
Catherine
C
Jansen-Dürr
Pidder
P
Zwerschke
Werner
W
D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology / Q000821:virology*; D000818:Animals; D045744:Cell Line, Tumor; D005260:Female; D006367:HeLa Cells; D052162:Human papillomavirus 16 / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D050725:Papillomavirus E7 Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D030361:Papillomavirus Infections / Q000378:metabolism / Q000473:pathology / Q000821:virology; D011817:Rabbits; D013347:Subcellular Fractions / Q000378:metabolism*; D002583:Uterine Cervical Neoplasms / Q000378:metabolism / Q000473:pathology / Q000821:virology*
D050725:Papillomavirus E7 Proteins; C059731:oncogene protein E7, Human papillomavirus type 16