Cryptic IGH/BCL2 rearrangements with variant FISH patterns in follicular lymphoma.
Follicular lymphoma (FL) is one of the most common non-Hodgkin lymphomas (NHL). Translocation t(14;18)(q32;q21) involving IGH and BCL2 genes represents its genetic hallmark. We present six cases of a series of 75 well diagnosed FL patients in which variant fluorescence in situ hybridization (FISH) patterns for this rearrangement were found. Moreover, G-banding cytogenetics and polymerase chain reaction (PCR) methods were unable to detect t(14;18)(q32;q21). According to our results, FISH is the best technique to define variant rearrangements of IGH/BCL2 genes and is important to detect it in cases with non-conclusive FL characteristics to avoid misdiagnosis with other NHL.
Laboratori de Citogenètica Molecular, Servei de Patologia, Hospital del Mar, Parc de Salut Mar, GRETNHE, IMIM-Hospital del Mar, Barcelona, Spain.
Cristina
C
Baró
Blanca
B
Espinet
Marta
M
Salido
Mar
M
García
Blanca
B
Sánchez
Lourdes
L
Florensa
Beatriz
B
Bellosillo
Sergi
S
Serrano
Francesc
F
Solé
D002869:Chromosome Aberrations; D002883:Chromosomes, Human, Pair 14 / Q000235:genetics; D002887:Chromosomes, Human, Pair 18 / Q000235:genetics; D050438:Genes, Immunoglobulin Heavy Chain / Q000235:genetics*; D019254:Genes, bcl-2 / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008224:Lymphoma, Follicular / Q000235:genetics*; D016133:Polymerase Chain Reaction; D014178:Translocation, Genetic / Q000235:genetics*