Benefit of magnesium-25 carrying porphyrin-fullerene nanoparticles in experimental diabetic neuropathy.
Diabetic neuropathy (DN) is a debilitating disorder occurring in most diabetic patients without a viable treatment yet. The present work examined the protective effect of (25)Mg-PMC(16) nanoparticle (porphyrin adducts of cyclohexil fullerene-C60) in a rat model of streptozotocin (STZ)-induced DN. (25)Mg-PMC(16) (0.5 lethal dose(50) [LD(50)]) was administered intravenously in two consecutive days before intraperitoneal injection of STZ (45 mg/kg). (24)Mg-PMC(16) and MgCl(2) were used as controls. Blood 2,3-diphosphoglycerate (2,3-DPG), oxidative stress biomarkers, adenosine triphosphate (ATP) level in dorsal root ganglion (DRG) neurons were determined as biomarkers of DN. Results indicated that 2,3-DPG and ATP decreased whereas oxidative stress increased by induction of DN which all were improved in (25)Mg-PMC(16)-treated animals. No significant changes were observed by administration of (24)Mg-PMC(16) or MgCl(2) in DN rats. It is concluded that in DN, oxidative stress initiates injuries to DRG neurons that finally results in death of neurons whereas administration of (25)Mg-PMC(16) by release of Mg and increasing ATP acts protectively.
Hosseini
Asieh
A
Faculty of Pharmacy, Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, Tehran, Iran.
Sharifzadeh
Mohammad
M
Rezayat
Seyed Mahdi
SM
Hassanzadeh
Gholamreza
G
Hassani
Shokoufeh
S
Baeeri
Maryam
M
Shetab-Bushehri
Vahid
V
Kuznetsov
Dmitry A
DA
Abdollahi
Mohammad
M
D019794:2,3-Diphosphoglycerate / Q000097:blood; D000244:Adenosine Diphosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D003929:Diabetic Neuropathies / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D016503:Drug Delivery Systems; D037741:Fullerenes / Q000737:chemistry; D005727:Ganglia, Spinal / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D007554:Isotopes / Q000008:administration & dosage; D008274:Magnesium / Q000008:administration & dosage* / Q000378:metabolism; D008297:Male; D050997:Nanomedicine; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry*; D018384:Oxidative Stress / Q000187:drug effects; D011166:Porphyrins / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar
D037741:Fullerenes; D007554:Isotopes; D011166:Porphyrins; D019794:2,3-Diphosphoglycerate; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D008274:Magnesium; C069837:fullerene C60