First report on the phylogeny of bovine norovirus in Turkey.
Bovine norovirus (BoNoV) is an important cause of diarrhea in calves and has been reported in several countries. The aims of this study were to investigate for the first time the presence of norovirus in Turkish calves by real-time reverse transcription-polymerase chain reaction (qRT-PCR) and to determine the phylogeny of any circulating strains. Fecal samples from 70 diarrheic calves were collected and analysed by SYBR Green qRT-PCR. BoNoV was detected in fecal samples from six calves. The capsid gene was partially sequenced, and phylogenetic analysis was performed. This showed that the six Turkish BoNoVs clustered with the GIII-2 prototype.
Department of Virology, University of Istanbul, Avcilar, Turkey. hyilmaz@istanbul.edu.tr
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D000818:Animals; D017250:Caliciviridae Infections / Q000453:epidemiology / Q000662:veterinary*; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000821:virology*; D003967:Diarrhea / Q000453:epidemiology / Q000662:veterinary / Q000821:virology; D005759:Gastroenteritis / Q000453:epidemiology / Q000662:veterinary / Q000821:virology; D029322:Norovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D010802:Phylogeny; D014421:Turkey / Q000453:epidemiology