Dynamics of intra-follicular glucose during luteinization of macaque ovarian follicles.
Glucose is important to the maturation of the oocyte and development of the embryo, while hyperglycemia results in profound reproductive and developmental consequences. However, the normal physiology of glucose in the ovary remains poorly understood. The goal of this study was to determine intra-follicular glucose dynamics during the periovulatory interval in non-human primates undergoing controlled ovarian stimulation protocols. Follicular fluid and mural granulosa cells were isolated before or up to 24h after an ovulatory hCG bolus, and the human granulosa-lutein cell line hGL5 was used. Intra-follicular glucose increased 3h after hCG, and remained at that level until 12h when levels decline back to pre-hCG concentrations. Pyruvate and lactate concentrations in the follicle were not strongly altered by hCG. Mural granulosa cell expression of hexokinase 1 and 2, and glucose-6-phosphate dehydrogenase mRNA decreased following hCG, while glycogen phosphorylase (liver form) increased following hCG. Glucose uptake by hGL5 cells was delayed until 24h following stimulation. In summary, intra-follicular glucose increases following an ovulatory stimulus and mural granulosa cells do not appear able to utilize it, sparing the glucose for the cumulus-oocyte complex.
Brogan
Rebecca S
RS
Department of Biology, Loyola University Maryland, 4501 N Charles St, Baltimore, MD 21201, United States.
MacGibeny
Margaret
M
Mix
Scott
S
Thompson
Christopher
C
Puttabyatappa
Muraly
M
VandeVoort
Catherine A
CA
Chaffin
Charles L
CL
D000818:Animals; D001826:Body Fluids / Q000737:chemistry; D002460:Cell Line; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D027342:Excitatory Amino Acid Transporter 2 / Q000235:genetics / Q000378:metabolism; D005260:Female; D005947:Glucose / Q000378:metabolism*; D006107:Granulosa Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D006801:Humans; D007328:Insulin / Q000494:pharmacology; D034461:Luteinization / Q000502:physiology*; D008251:Macaca / Q000033:anatomy & histology / Q000502:physiology; D009865:Oocytes / Q000166:cytology / Q000502:physiology; D006080:Ovarian Follicle / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*
D006063:Chorionic Gonadotropin; D027342:Excitatory Amino Acid Transporter 2; D007328:Insulin; D005947:Glucose