Efficient CNS gene delivery by intravenous injection.
We administered recombinant SV40-derived viral vectors (rSV40s) intravenously to mice with or without prior intraperitoneal injection of mannitol to deliver transgenes to the central nervous system (CNS). We detected transgene-expressing cells (mainly neurons) most prominently in the cortex and spinal cord; prior intraperitoneal mannitol injection increased CNS gene delivery tenfold. Intravenous injection of rSV40s, particularly with mannitol pretreatment, resulted in extensive expression of multiple transgenes throughout the CNS.
Department of Pathology, Thomas Jefferson University, Philadelphia, Pennsylvania, USA. jplouboutin@hotmail.com
Jean-Pierre
JP
Louboutin
Alena A
AA
Chekmasova
Elena
E
Marusich
J Roy
JR
Chowdhury
David S
DS
Strayer
D000818:Animals; D019556:COS Cells; D002540:Cerebral Cortex / Q000378:metabolism*; D002522:Chlorocebus aethiops; D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D007275:Injections, Intravenous; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D013539:Simian virus 40 / Q000235:genetics*; D013116:Spinal Cord / Q000378:metabolism*; D019076:Transgenes