Ultra-deep sequencing reveals the microRNA expression pattern of the human stomach.
BACKGROUND
While microRNAs (miRNAs) play important roles in tissue differentiation and in maintaining basal physiology, little is known about the miRNA expression levels in stomach tissue. Alterations in the miRNA profile can lead to cell deregulation, which can induce neoplasia.


RESULTS
A small RNA library of stomach tissue was sequenced using high-throughput SOLiD sequencing technology. We obtained 261,274 quality reads with perfect matches to the human miRnome, and 42% of known miRNAs were identified. Digital Gene Expression profiling (DGE) was performed based on read abundance and showed that fifteen miRNAs were highly expressed in gastric tissue. Subsequently, the expression of these miRNAs was validated in 10 healthy individuals by RT-PCR showed a significant correlation of 83.97% (P<0.05). Six miRNAs showed a low variable pattern of expression (miR-29b, miR-29c, miR-19b, miR-31, miR-148a, miR-451) and could be considered part of the expression pattern of the healthy gastric tissue.


CONCLUSIONS
This study aimed to validate normal miRNA profiles of human gastric tissue to establish a reference profile for healthy individuals. Determining the regulatory processes acting in the stomach will be important in the fight against gastric cancer, which is the second-leading cause of cancer mortality worldwide.
Ribeiro-dos-Santos
Ândrea
Â
Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, Brazil. akely@ufpa.br
Khayat
André S
AS
Silva
Artur
A
Alencar
Dayse O
DO
Lobato
Jessé
J
Luz
Larissa
L
Pinheiro
Daniel G
DG
Varuzza
Leonardo
L
Assumpção
Monica
M
Assumpção
Paulo
P
Santos
Sidney
S
Zanette
Dalila L
DL
Silva
Wilson A
WA
Burbano
Rommel
R
Darnet
Sylvain
S
D005753:Gastric Mucosa / Q000378:metabolism*; D020869:Gene Expression Profiling; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017423:Sequence Analysis, RNA
D035683:MicroRNAs