Multipotent stromal cells for autologous cell therapy approaches in the guinea pig model.
Multipotent stromal cells have become of increasing interest due to their potential to provide therapeutic approaches for autologous tissue repair. However, these cells are not well defined in the guinea pig, which represents an important model in hearing research. Adipose-tissue-derived stem cells (ADSC) and bone-marrow-derived stem cells (BMSC) were isolated from different donor sites, and growth curves were generated to judge the proliferation potential. Adipogenic, chondrogenic and osteogenic differentiation was induced and confirmed histologically. Finally, the capability of guinea pig ADSC to differentiate into neuron-like cells was investigated. With regard to the expansion potential, total cell number and doubling time, ADSC from the neck were the most suitable cells of the tested donor sites. Both ADSC and BMSC showed nearly identical behaviour and ability to undergo multilineage differentiation. Thus, we identified ADSC from the neck as a promising cell source for autologous cell-based approaches in hearing research using the guinea pig model.
Department of Otorhinolaryngology, Plastic, Aesthetic and Reconstructive Head and Neck Surgery, University of Wuerzburg, Wuerzburg, Germany. froelich_k@klinik.uni-wuerzburg.de
Katrin
K
Frölich
Agmal
A
Scherzed
Robert
R
Mlynski
Antje
A
Technau
Rudolf
R
Hagen
Norbert
N
Kleinsasser
Andreas
A
Radeloff
D050156:Adipogenesis / Q000502:physiology; D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D020219:Chondrogenesis / Q000502:physiology; D005260:Female; D006168:Guinea Pigs; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D045164:Mesenchymal Stem Cell Transplantation; D023421:Models, Animal; D039902:Multipotent Stem Cells / Q000166:cytology*; D010012:Osteogenesis / Q000502:physiology; D017154:Stromal Cells / Q000166:cytology*; D020858:Tissue and Organ Harvesting / Q000379:methods; D014182:Transplantation, Autologous