Achromobacter marplatensis sp. nov., isolated from a pentachlorophenol-contaminated soil.
A polyphasic taxonomic approach was applied to the study of a Gram-negative bacterium (B2(T)) isolated from soil by selective enrichment with pentachlorophenol. 16S rRNA gene sequence analysis of strain B2(T) showed that the strain belongs to the genus Achromobacter within the Betaproteobacteria. The 16S rRNA gene sequence displayed more than 99 % similarity to the sequences of the type strains of all species of Achromobacter, with the highest sequence similarity to those of Achromobacter spanius CCM 7183(T) and A. piechaudii CCM 2986(T) (99.8 %). On the basis of phylogenetic analysis, genomic DNA-DNA relatedness and phenotypic characteristics, including chemotaxonomic (cellular fatty acid profile) analysis, a novel species is proposed, Achromobacter marplatensis sp. nov., with the type strain B2(T) ( = CCM 7608(T)  = CCUG 56371(T)  = CECT 7342(T)).
Microbiologia, Departament de Biologia, Universitat de les Illes Balears, and Institut Mediterrani d'Estudis Avançats (CSIC-UIB), 07122 Palma de Mallorca, Illes Balears, Spain.
Margarita
M
Gomila
Division of Microbiology, Department of Experimental Biology, Faculty of Science, Masaryk University, Tvrdého 14, 60200 Brno, Czech Republic.
Ludmila
L
Tvrzová
Division of Microbiology, Department of Experimental Biology, Faculty of Science, Masaryk University, Tvrdého 14, 60200 Brno, Czech Republic.
Andrea
A
Teshim
Czech Collection of Microorganisms, Department of Experimental Biology, Faculty of Science, Masaryk University, Tvrdého 14, 60200 Brno, Czech Republic.
Ivo
I
Sedláček
Center for Food Safety and Applied Nutrition, Food and Drug Administration, College Park, MD, USA.
Narjol
N
González-Escalona
Division of Functional Genomics and Proteomics, Department of Experimental Biology, Faculty of Science, Masaryk University, Kamenice 5, 62500 Brno, Czech Republic.
Zbyněk
Z
Zdráhal
Division of Functional Genomics and Proteomics, Department of Experimental Biology, Faculty of Science, Masaryk University, Kamenice 5, 62500 Brno, Czech Republic.
Ondrej
O
Šedo
Universidad Nacional de Mar del Plata, 7600 Mar del Plata, Buenos Aires, Argentina.
Jorge Froilán
JF
González
Institut Universitari d'Investigació en Ciències de la Salut (IUNICS-UIB), Campus UIB, 07122 Palma de Mallorca, Spain.|Microbiologia, Departament de Biologia, Universitat de les Illes Balears, and Institut Mediterrani d'Estudis Avançats (CSIC-UIB), 07122 Palma de Mallorca, Illes Balears, Spain.
Antonio
A
Bennasar
Sahlgrenska Academy of the University of Gothenburg, Gothenburg, Sweden.|Culture Collection University of Gothenburg (CCUG), Department of Clinical Bacteriology, Sahlgrenska University Hospital, Gothenburg, Sweden.
Edward R B
ERB
Moore
Microbiologia, Departament de Biologia, Universitat de les Illes Balears, and Institut Mediterrani d'Estudis Avançats (CSIC-UIB), 07122 Palma de Mallorca, Illes Balears, Spain.
Jorge
J
Lalucat
Universidad Nacional de Mar del Plata, 7600 Mar del Plata, Buenos Aires, Argentina.
Silvia E
SE
Murialdo
D042402:Achromobacter / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D015373:Bacterial Typing Techniques; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010416:Pentachlorophenol / Q000378:metabolism*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants; D010416:Pentachlorophenol