D-chiro-inositol affects accumulation of raffinose family oligosaccharides in developing embryos of Pisum sativum.
Developing garden pea embryos are able to take up exogenously applied cyclitols: myo-inositol, which naturally occurs in pea, and two cyclitols absent in pea plants: d-chiro-inositol and d-pinitol. The competition in the uptake of cyclitols by pea embryo, insensitivity to glucose and sucrose, and susceptibility to inhibitor(s) of H(+)-symporters (e.g. CCCP and antimycin A) suggest that a common cyclitol transporter is involved. Both d-chiro-inositol and d-pinitol can be translocated through the pea plant to developing embryos. During seed maturation drying, they are used for synthesis of mainly mono-galactosides, such as fagopyritol B1 and galactosyl pinitol A. Accumulation of d-chiro-inositol (and formation of fagopyritols), but not d-pinitol, strongly reduces accumulation of verbascose, the main raffinose oligosaccharide in pea seeds. The reasons for the observed changes are discussed.
Lahuta
Lesław B
LB
Department of Plant Physiology and Biotechnology, University of Warmia and Mazury in Olsztyn, Olsztyn, Oczapowskiego, Poland. lahuta@uwm.edu.pl
Dzik
Tomasz
T
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000968:Antimycin A / Q000494:pharmacology; D001692:Biological Transport / Q000187:drug effects; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology; D004187:Disaccharides / Q000032:analysis / Q000378:metabolism; D007294:Inositol / Q000031:analogs & derivatives / Q000032:analysis / Q000494:pharmacology*; D009844:Oligosaccharides / Q000032:analysis / Q000378:metabolism*; D018532:Pisum sativum / Q000187:drug effects / Q000196:embryology / Q000378:metabolism*; D011887:Raffinose / Q000032:analysis / Q000378:metabolism; D012639:Seeds / Q000187:drug effects / Q000378:metabolism*; D013997:Time Factors; D014475:Uncoupling Agents / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D004187:Disaccharides; D009844:Oligosaccharides; D014475:Uncoupling Agents; C112483:fagopyritol B1; C005695:stachyose; C021730:pinitol; D007294:Inositol; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D000968:Antimycin A; D011887:Raffinose