Measurement of bioavailability: measuring absorption through skin in vivo in rats and humans.
Absorption through the skin is a route of exposure to a wide variety of therapeutic and/or environmental compounds. In vivo assays are advantageous in that they retain intact epidermal and dermal structures and thus reflect a more normal situation. The test compound is applied to the skin in a protected area for a specified period of time. At the end of the incubation, skin, tissues, and excreta are assessed for the presence of the test compound. The assay can also be performed with volatile compounds. When performed using laboratory animals, it is possible to assess the distribution of the compound throughout the body. If human volunteers are studied such analysis is limited to the skin, blood, and excreta.
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Huntingdon Life Science, Huntingdon, England.
Hawkins
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D000818:Animals; D001682:Biological Availability; D004785:Environmental Pollutants / Q000493:pharmacokinetics / Q000633:toxicity; D006801:Humans; D004364:Pharmaceutical Preparations / Q000378:metabolism; D051381:Rats; D012867:Skin / Q000378:metabolism*; D012869:Skin Absorption; D013045:Species Specificity; D046509:Tissue Culture Techniques / Q000295:instrumentation / Q000379:methods / Q000592:standards; D014116:Toxicology / Q000295:instrumentation / Q000379:methods* / Q000592:standards
D004785:Environmental Pollutants; D004364:Pharmaceutical Preparations