[Determination of ethyl glucuronide in blood and urine by LC-MS/MS].
OBJECTIVE
To develop a method for determining ethyl glucuronide (EtG) in blood and urine by liquid chromatograph coupled with tandem mass spectrometer (LC-MS/MS).


METHODS
After blood and urine de-proteined by acetonitrile, the supernate obtained from a centrifuge was analyzed by LC-MS/MS.


RESULTS
Determination limit of EtG in both blood and urine was 0.05 pg/mL, with a linear range of 0.10-5.00 microg/mL (r > 0.999). Accuracy in both matrixes was 95%-109%. Inter- and intra-day RSD were less than 12%. The method showed an excellent performance when it was used to analyze authentic blood and urine samples for EtG.


CONCLUSIONS
The method is capable for blood and urine EtG analysis.
Shanghai Medical College, Fudan University, Shanghai 200032, China.
Hui
H
Zhao
Xian-yi
XY
Zhuo
Hui
H
Yan
Bao-hua
BH
Shen
D000437:Alcoholism / Q000097:blood / Q000652:urine; D015415:Biomarkers / Q000097:blood / Q000652:urine; D002853:Chromatography, Liquid / Q000379:methods; D000431:Ethanol / Q000378:metabolism*; D053593:Forensic Toxicology / Q000379:methods; D005965:Glucuronates / Q000097:blood* / Q000652:urine*; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D015813:Substance Abuse Detection / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
D015415:Biomarkers; D005965:Glucuronates; C093924:ethyl glucuronide; D000431:Ethanol