Multi-enzyme logic network architectures for assessing injuries: digital processing of biomarkers.
A multi-enzyme biocatalytic cascade processing simultaneously five biomarkers characteristic of traumatic brain injury (TBI) and soft tissue injury (STI) was developed. The system operates as a digital biosensor based on concerted function of 8 Boolean AND logic gates, resulting in the decision about the physiological conditions based on the logic analysis of complex patterns of the biomarkers. The system represents the first example of a multi-step/multi-enzyme biosensor with the built-in logic for the analysis of complex combinations of biochemical inputs. The approach is based on recent advances in enzyme-based biocomputing systems and the present paper demonstrates the potential applicability of biocomputing for developing novel digital biosensor networks.
Halámek
Jan
J
Department of Chemistry and Biomolecular Science, Clarkson University, Potsdam, NY 13699-5810, USA.
Bocharova
Vera
V
Chinnapareddy
Soujanya
S
Windmiller
Joshua Ray
JR
Strack
Guinevere
G
Chuang
Min-Chieh
MC
Zhou
Jian
J
Santhosh
Padmanabhan
P
Ramirez
Gabriela V
GV
Arugula
Mary A
MA
Wang
Joseph
J
Katz
Evgeny
E
D000818:Animals; D055162:Biocatalysis; D015415:Biomarkers / Q000032:analysis*; D001930:Brain Injuries / Q000175:diagnosis* / Q000201:enzymology*; D008128:Logic; D009097:Multienzyme Complexes / Q000378:metabolism*; D011817:Rabbits; D012815:Signal Processing, Computer-Assisted; D017695:Soft Tissue Injuries / Q000175:diagnosis* / Q000201:enzymology; D034421:Sus scrofa
D015415:Biomarkers; D009097:Multienzyme Complexes