Multiple biological complex of alkaline extract of the leaves of Sasa senanensis Rehder.
Previous studies have shown anti-inflammatory potential of alkaline extract of the leaves of Sasa senanensis Rehder (SE). The aim of the present study was to clarity the molecular entity of SE, using various fractionation methods. SE inhibited the production of nitric oxide (NO), but not tumour necrosis factor-α by lipopolysaccharide (LPS)-stimulated mouse macrophage-like cells. Lignin carbohydrate complex prepared from SE inhibited the NO production to a comparable extent with SE, whereas chlorophyllin was more active. On successive extraction with organic solvents, nearly 90% of SE components, including chlorophyllin, were recovered from the aqueous layer. Anti-HIV activity of SE was comparable with that of lignin-carbohydrate complex, and much higher than that of chlorophyllin and n-butanol extract fractions. The CYP3A inhibitory activity of SE was significantly lower than that of grapefruit juice and chlorophyllin. Oral administration of SE slightly reduced the number of oral bacteria. When SE was applied to HPLC, nearly 70% of SE components were eluted as a single peak. These data suggest that multiple components of SE may be associated with each other in the native state or after extraction with alkaline solution.
Sakagami
Hiroshi
H
Division of Pharmacology, Department of Diagnostic and Therapeutic Sciences, Meikai University School of Dentistry, Sakado, Saitama 350-0283, Japan. sakagami@dent.meikai.ac.jp
Zhou
Li
L
Kawano
Michiyo
M
Thet
May Maw
MM
Tanaka
Shoji
S
Machino
Mamoru
M
Amano
Shigeru
S
Kuroshita
Reina
R
Watanabe
Shigeru
S
Chu
Qing
Q
Wang
Qin-Tao
QT
Kanamoto
Taisei
T
Terakubo
Shigemi
S
Nakashima
Hideki
H
Sekine
Keisuke
K
Shirataki
Yoshiaki
Y
Zhang
Chang-Hao
CH
Uesawa
Yoshihiro
Y
Mohri
Kiminori
K
Kitajima
Madoka
M
Oizumi
Hiroshi
H
Oizumi
Takaaki
T
D000468:Alkalies / Q000008:administration & dosage*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage*; D016866:Bacteroidaceae Infections / Q000188:drug therapy / Q000276:immunology / Q000382:microbiology; D002460:Cell Line; D002735:Chlorophyllides / Q000494:pharmacology; D032083:Citrus paradisi / Q000737:chemistry; D015658:HIV Infections / Q000188:drug therapy; D006801:Humans; D008031:Lignin / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051379:Mice; D008862:Microsomes, Liver / Q000187:drug effects; D009569:Nitric Oxide / Q000378:metabolism; D010936:Plant Extracts / Q000008:administration & dosage*; D016966:Porphyromonas gingivalis / Q000187:drug effects / Q000254:growth & development; D051381:Rats; D031787:Sasa / Q000737:chemistry*; D013280:Stomatitis / Q000188:drug therapy* / Q000276:immunology / Q000382:microbiology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D000468:Alkalies; D000893:Anti-Inflammatory Agents; D002735:Chlorophyllides; D008070:Lipopolysaccharides; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; D008031:Lignin; C007020:chlorophyllin