Interaction of Nectarin 4 with a fungal protein triggers a microbial surveillance and defense mechanism in nectar.
Understanding the biochemical mechanisms by which plants respond to microbial infection is a fundamental goal of plant science. Extracellular dermal glycoproteins (EDGPs) are widely expressed in plant tissues and have been implicated in plant defense responses. Although EDGPs are known to interact with fungal proteins, the downstream effects of these interactions are poorly understood. To gain insight into these phenomena, we used tobacco floral nectar as a model system to identify a mechanism by which the EDGP known as Nectarin IV (NEC4) functions as pathogen surveillance molecule. Our data demonstrates that the interaction of NEC4 with a fungal endoglucanase (XEG) promotes the catalytic activity of Nectarin V (NEC5), which catalyzes the conversion of glucose and molecular oxygen to gluconic acid and H(2)O(2). Significantly enhanced NEC5 activity was observed when XEG was added to nectar or nectarin solutions that contain NEC4. This response was also observed when the purified NEC4:XEG complex was added to NEC4-depleted nectarin solutions, which did not respond to XEG alone. These results indicate that formation of the NEC4:XEG complex is a key step leading to induction of NEC5 activity in floral nectar, resulting in an increase in concentrations of reactive oxygen species (ROS), which are known to inhibit microbial growth directly and activate signal transduction pathways that induce innate immunity responses in the plant.
Harper
April D
AD
Department of Biochemistry, Complex Carbohydrate Research Center, University of Georgia, 315 Riverbend Road, Athens, GA 30602, USA.
Stalnaker
Stephanie H
SH
Wells
Lance
L
Darvill
Alan
A
Thornburg
Robert
R
York
William S
WS
D002480:Cellulase / Q000037:antagonists & inhibitors; D005656:Fungal Proteins / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D005936:Glucans / Q000032:analysis / Q000378:metabolism; D005942:Gluconates / Q000378:metabolism; D005949:Glucose Oxidase / Q000187:drug effects / Q000378:metabolism*; D006023:Glycoproteins / Q000378:metabolism*; D057048:Plant Nectar / Q000737:chemistry / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000032:analysis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014026:Nicotiana / Q000737:chemistry* / Q000201:enzymology; D014990:Xylans / Q000032:analysis / Q000378:metabolism
D005656:Fungal Proteins; D005936:Glucans; D005942:Gluconates; D006023:Glycoproteins; D057048:Plant Nectar; D010940:Plant Proteins; D017382:Reactive Oxygen Species; D014990:Xylans; C555191:nectarin 4 protein, Nicotiana tabacum; C029353:xyloglucan; D005949:Glucose Oxidase; C555193:nectarin V protein, Nicotiana tabacum; D002480:Cellulase; C030691:gluconic acid