Phytic acid protects porcine intestinal epithelial cells from deoxynivalenol (DON) cytotoxicity.
The purpose of this study was to evaluate the effects of phytic acid (IP(6)) as a possible inhibitor of cellular damage induced by toxic substances such as mycotoxins on a porcine intestinal epithelial cell line (IPEC-1). We first observed that a dose of 5 mM phytic acid decreases cell viability and transepithelial electrical resistance (TEER) of cell monolayer. We next investigate the effect of non-cytotoxic dose of phytic acid on the deoxinivalenol (DON) induced decreased TEER. We showed that treatment with 0.5 mM or 1.0 mM phytic acid restores the decrease in TEER caused by 25 μM DON. In conclusion this study demonstrates that phytic acid decreased the negative effects of deoxynivalenol on the membrane integrity of the IPEC-1 intestinal epithelial cell line.
Pacheco
Graziela Drociunas
GD
Universidade Estadual de Londrina, Centro de Ciências Agrárias, Campus Universitário, Londrina, CX Postal 6001, Brazil.
Silva
Caio Abércio da
CA
Pinton
Philippe
P
Oswald
Isabelle P
IP
Bracarense
Ana Paula Frederico Rodrigues Loureiro
AP
D000818:Animals; D002463:Cell Membrane Permeability / Q000187:drug effects / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D055724:Electrophysiological Phenomena / Q000187:drug effects; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000473:pathology / Q000502:physiology; D007421:Intestine, Small / Q000187:drug effects* / Q000473:pathology / Q000502:physiology; D018408:Patch-Clamp Techniques; D010833:Phytic Acid / Q000494:pharmacology*; D013552:Swine; D014255:Trichothecenes / Q000633:toxicity*
D014255:Trichothecenes; D010833:Phytic Acid; C007262:deoxynivalenol