Rapid isolation of intact chloroplasts from spinach leaves.
In this chapter, a rapid method to isolate intact chloroplasts from spinach leaves is described. Intact chloroplasts are isolated using two short centrifugation steps and avoiding the use of percoll gradient. Intactness of chloroplast is evaluated by the inability of potassium ferricyanide to enter inside the chloroplasts and to act as an electron acceptor for photosystem II.
Joly
David
D
Département de chimie biologie (GRBV), Université du Québec à Trois-Rivières, Trois-Rivières, QC, Canada.
Carpentier
Robert
R
D002458:Cell Fractionation / Q000379:methods*; D002498:Centrifugation / Q000379:methods*; D002734:Chlorophyll / Q000378:metabolism; D002736:Chloroplasts / Q000378:metabolism; D009997:Osmotic Pressure; D010100:Oxygen / Q000378:metabolism; D018515:Plant Leaves / Q000166:cytology* / Q000378:metabolism; D018724:Spinacia oleracea / Q000166:cytology* / Q000378:metabolism; D013997:Time Factors
D002734:Chlorophyll; D010100:Oxygen