Pandoraea oxalativorans sp. nov., Pandoraea faecigallinarum sp. nov. and Pandoraea vervacti sp. nov., isolated from oxalate-enriched culture.
Five isolates, designated TA2, TA4, TA25(T), KOx(T) and NS15(T) were isolated in previous studies by enrichment in mineral medium with potassium oxalate as the sole carbon source and were characterized using a polyphasic approach. The isolates were Gram-reaction-negative, aerobic, non-spore-forming rods. Phylogenetic analyses based on 16S rRNA and DNA gyrase B subunit (gyrB) gene sequences confirmed that the isolates belonged to the genus Pandoraea and were most closely related to Pandoraea sputorum and Pandoraea pnomenusa (97.2-99.7 % 16S rRNA gene sequence similarity). The isolates could be differentiated from their closest relatives on the basis of several phenotypic characteristics. The major cellular fatty acid profiles of the isolates comprised C₁₆:₀, C₁₈:₁ω7c, C₁₇:₀ cyclo and summed feature 3 (C₁₆:₁ω7c and/or iso-C₁₅:₀ 2-OH). On the basis of DNA-DNA hybridization studies and phylogenetic analyses, the isolates represent three novel species within the genus Pandoraea, for which the names Pandoraea oxalativorans sp. nov. (TA25(T)  = NBRC 106091(T)  = CCM 7677(T)  = DSM 23570(T)), Pandoraea faecigallinarum sp. nov. (KOx(T)  = NBRC 106092(T)  = CCM 2766(T)  = DSM 23572(T)) and Pandoraea vervacti sp. nov. (NS15(T)  = NBRC 106088(T)  = CCM 7667(T)  = DSM 23571(T)) are proposed.
Sahin
Nurettin
N
Egitim Fakultesi, Mugla University, Mugla, Turkey.
Tani
Akio
A
Institute of Plant Science and Resources, Okayama University, Okayama, Japan.
Kotan
Recep
R
Department of Plant Protection, Faculty of Agriculture, Ataturk University, Erzurum, Turkey.
Sedláček
Ivo
I
Czech Collection of Microorganisms, Department of Experimental Biology, Faculty of Science, Masaryk University, Brno, Czech Republic.
Kimbara
Kazuhide
K
Institute of Plant Science and Resources, Okayama University, Okayama, Japan.
Tamer
Abdurrahman U
AU
Faculty of Science and Arts, Celal Bayar University, Manisa, Turkey.
D000332:Aerobiosis; D015373:Bacterial Typing Techniques; D042521:Burkholderiaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D016000:Cluster Analysis; D003470:Culture Media / Q000737:chemistry; D027081:DNA Gyrase / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010070:Oxalates / Q000378:metabolism*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
D003470:Culture Media; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010070:Oxalates; D012336:RNA, Ribosomal, 16S; D027081:DNA Gyrase