Hypoxia-sensitive fluorescent probes for in vivo real-time fluorescence imaging of acute ischemia.
Based on the findings that the azo functional group has excellent properties as the hypoxia-sensor moiety, we developed hypoxia-sensitive near-infrared fluorescent probes in which a large fluorescence increase is triggered by the cleavage of an azo bond. The probes were used for fluorescence imaging of hypoxic cells and real-time monitoring of ischemia in the liver and kidney of live mice.
Kiyose
Kazuki
K
Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
Hanaoka
Kenjiro
K
Oushiki
Daihi
D
Nakamura
Tomomi
T
Kajimura
Mayumi
M
Suematsu
Makoto
M
Nishimatsu
Hiroaki
H
Yamane
Takehiro
T
Terai
Takuya
T
Hirata
Yasunobu
Y
Nagano
Tetsuo
T
D000208:Acute Disease; D000818:Animals; D001391:Azo Compounds / Q000737:chemistry*; D045744:Cell Line, Tumor; D005453:Fluorescence; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D000860:Hypoxia / Q000175:diagnosis*; D007511:Ischemia / Q000175:diagnosis*; D051379:Mice; D010100:Oxygen / Q000737:chemistry; D019265:Spectroscopy, Near-Infrared / Q000379:methods*
D001391:Azo Compounds; D005456:Fluorescent Dyes; C009850:azobenzene; D010100:Oxygen