From single genes to gene networks: high-throughput-high-content screening for neurological disease.
Neuronal development, function, and the subsequent degeneration of the brain are still an enigma in both the normal and pathologic states, and there is an urgent need to find better targets for developing therapeutic intervention. Current techniques to deconstruct the architecture of brain and disease-related pathways are best suited for following up on single genes but would take an impractical amount of time for the leads from the current wave of genetic and genomic data. New technical developments have made combined high-throughput-high-content (HT-HC) cellular screens possible, which have the potential to contextualize the information, gathered from a combination of genetic and genomic approaches, into networks and functional biology and can be utilized for the identification of therapeutic targets. Herein we discuss the potential impact of HT-HC cellular screens on medical neuroscience.
Jain
Shushant
S
Department of Clinical Genetics, VU University Medical Center Amsterdam, The Netherlands.
Heutink
Peter
P
D019295:Computational Biology / Q000379:methods; D005786:Gene Expression Regulation / Q000235:genetics; D053263:Gene Regulatory Networks / Q000235:genetics / Q000502:physiology*; D005796:Genes; D005820:Genetic Testing / Q000379:methods*; D006801:Humans; D046228:Microarray Analysis / Q000379:methods; D008954:Models, Biological; D009422:Nervous System Diseases / Q000175:diagnosis* / Q000235:genetics*