Inhibitory activities of cyanidin and its glycosides and synergistic effect with acarbose against intestinal α-glucosidase and pancreatic α-amylase.
Cyanidin and its glycosides are naturally dietary pigments which have been indicated as promising candidates to have potential benefits to humans, especially in the prevention and treatment of diabetes mellitus. We investigated the structure activity relationships of cyanidin and its glycosides to inhibit intestinal α-glucosidases and pancreatic α-amylase in vitro. The results found that cyanidin and its glycosides are more specific inhibitors of intestinal sucrase than intestinal maltase. Cyanidin-3-galactoside and cyanidin-3-glucoside were the most potent inhibitors against intestinal sucrase and pancreatic α-amylase with IC(50) values of 0.50 ± 0.05 and 0.30 ± 0.01 mM, respectively. Our findings indicate that the structural difference between glucose and galactose at the 3-O-position of cyanidin was an important factor for modulating the inhibition of intestinal sucrase and pancreatic α-amylase. The combination of cyandin-3-glucoside, cyanidin-3- galactoside or cyanidin-3,5-diglucosides with a low concentration of acarbose showed synergistic inhibition on intestinal maltase and sucrase. The synergistic inhibition was also found for a combination of cyanidin or cyanidin-3-glucoside with a low concentration of acarbose. The findings could provide a new insight into a use for the naturally occurring intestinal α-glucosidase and pancreatic α-amylase inhibitors for the prevention and treatment of diabetes and its complications.
Department of Pharmacology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand; E-Mails: akkara_sarin@hotmail.com (S.A.); piyawanch@yahoo.com (P.C.); sirintorn.y@chula.ac.th (S.Y.).
Sarinya
S
Akkarachiyasit
Piyawan
P
Charoenlertkul
Sirintorn
S
Yibchok-Anun
Sirichai
S
Adisakwattana
D020909:Acarbose / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D000872:Anthocyanins / Q000737:chemistry / Q000494:pharmacology*; D004357:Drug Synergism; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D065089:Glycoside Hydrolase Inhibitors; D006027:Glycosides / Q000737:chemistry / Q000494:pharmacology*; D007422:Intestines / Q000201:enzymology; D055292:Pancreatic alpha-Amylases / Q000037:antagonists & inhibitors* / Q000737:chemistry; D051381:Rats; D013379:Substrate Specificity; D013552:Swine; D000520:alpha-Glucosidases / Q000737:chemistry
D000872:Anthocyanins; D004791:Enzyme Inhibitors; D065089:Glycoside Hydrolase Inhibitors; D006027:Glycosides; C017154:cyanidin; D055292:Pancreatic alpha-Amylases; D000520:alpha-Glucosidases; D020909:Acarbose