Building mitotic chromosomes.
Mitotic chromosomes are the iconic structures into which the genome is packaged to ensure its accurate segregation during mitosis. Although they have appeared on countless journal cover illustrations, there remains no consensus on how the chromatin fiber is packaged during mitosis. In fact, work in recent years has both added to existing controversies and sparked new ones. By contrast, there has been very significant progress in determining the protein composition of isolated mitotic chromosomes. Here, we discuss recent studies of chromosome organization and provide an in depth description of the latest proteomics studies, which have at last provided us with a definitive proteome for vertebrate chromosomes.
Ohta
Shinya
S
Wellcome Trust Centre for Cell Biology, University of Edinburgh, Michael Swann Building, King's Buildings, Mayfield Road, Edinburgh EH9 3JR, Scotland, UK.
Wood
Laura
L
Bukowski-Wills
Jimi-Carlo
JC
Rappsilber
Juri
J
Earnshaw
William C
WC
D000818:Animals; D002875:Chromosomes / Q000378:metabolism*; D006801:Humans; D008938:Mitosis; D008958:Models, Molecular