Near full-length sequence analysis of a novel HIV-1 C/CRF01_AE recombinant variant from Yunnan, China.
Recombination contributes substantially to the overall genetic complexity of HIV-1, especially in populations in which multiple subtypes circulate. Yunnan is a province in China with most severely HIV epidemic where many subtypes and CRFs of HIV-1 are circulating. In this study, we report a novel HIV-1 recombination involving C and CRF01_AE. The near full-length genome was reverse transcripted and amplified in two halves with the 1 kb overlap regions. The PCR products were sequenced directly after purification. Sequence analysis showed that the majority of the genome was CRF01_AE, three fragments of C subtype were inserted along the genome in gag and pol region. This is the first report of a novel recombination involving subtype C and CRF01_AE in Yunnan, China. More work is needed to check the epidemiologic significance of the new recombination.
Li
Lin
L
Department of AIDS Research, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China.
Chen
Lili
L
Yang
Shaomin
S
Liu
Yongjian
Y
Li
Jianjian
J
Wang
Zheng
Z
Yang
Bihui
B
Li
Hanping
H
Bao
Zuoyi
Z
Zhuang
Daomin
D
Liu
Siyang
S
Li
Jingyun
J
D002681:China; D016000:Cluster Analysis; D016679:Genome, Viral; D005838:Genotype; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA
D012367:RNA, Viral