[Effect of oral glutamine on intestinal barrier function in young rats with endotoxemia].
OBJECTIVE
To study the effect of glutamine on intestinal barrier function by examining the changes of plasma D-lactic levels and diamine oxidase (DAO) levels in plasma and intestinal tissue after glutamine intervention in young rats with endotoxemia.


METHODS
Eighty 18-day-old rats were randomly divided into endotoxemia and glutamine intervention groups (n=40 each). Endotoxemia was induced by lipopolysaccharide (LPS) injection. Plasma and small intestine homogenate were collected 1.5, 6, 24 and 72 hrs and 7 days after LPS injection. The glutamine intervention group was immediately administered with oral glutamine (2 g/kg) after LPS injection. Afterwards, glutamine was administered once daily. Plasma D-lactic and DAO levels and intestinal DAO levels were measured.


RESULTS
Plasma DAO activity in the glutamine intervention group was significantly lower than that in the endotoxemia group 6 and 72 hrs after LPS injection (P<0.05). In contrast, the intestinal DAO activity in the glutamine intervention group was significantly higher than that in the endotoxemia group 6, 24 and 72 hrs and 7 days after LPS injection (P<0.05 or 0.01). Plasma D-lactic levels in the glutamine intervention group were significantly lower than those in the endotoxemia group 6, 24 and 72 hrs and 7 days after LPS injection (P<0.01).


CONCLUSIONS
Glutamine may reduce the permeability of intestinal mucosa, and thus provides protective effects on intestinal barrier function in rats with endotoxemia.
Department of Neonatology, First Affiliated Hospital of Xinxiang Medical University, Weihui, Henan, China.
Xiao
X
Chang
Lin-Lin
LL
Wang
Shu-Jun
SJ
Lian
Qing
Q
Tang
Ping
P
Chen
Hua
H
Wang
D000284:Administration, Oral; D006631:Amine Oxidase (Copper-Containing) / Q000378:metabolism; D000818:Animals; D001522:Behavior, Animal; D019446:Endotoxemia / Q000378:metabolism*; D005260:Female; D005973:Glutamine / Q000008:administration & dosage / Q000494:pharmacology*; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000187:drug effects*; D019344:Lactic Acid / Q000097:blood; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D005973:Glutamine; D019344:Lactic Acid; D006631:Amine Oxidase (Copper-Containing)