Bacteriocin-like substance from Bacillus amyloliquefaciens shows remarkable inhibition of Acanthamoeba polyphaga.
The effectiveness of a bacteriocin-like substance (BLS) produced by Bacillus amyloliquefaciens was tested against Acanthamoeba polyphaga strains, and its cytotoxic potential on Vero cells was investigated. Amebicidal activity of the purified BLS was tested by plate bioassays with concentrations ranging from 12.5 to 6,400 AU mL(-1). Damage to A. pholyphaga cells was monitored using an inverted microscope and counted in a Fuchs-Rosenthal chamber after 24, 48, and 72 h. According to the results obtained, the BLS showed remarkable amebicidal and amebostatic effect on A. polyphaga and showed no cytotoxicity on the Vero cells. These results may have great relevance in the development of new acanthamoebicidal compounds.
Benitez
Lisianne Brittes
LB
Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
Caumo
Karin
K
Brandelli
Adriano
A
Rott
Marilise Brittes
MB
D000048:Acanthamoeba / Q000187:drug effects* / Q000254:growth & development*; D000563:Amebicides / Q000494:pharmacology*; D000818:Animals; D001407:Bacillus / Q000737:chemistry*; D001426:Bacterial Proteins / Q000494:pharmacology; D001430:Bacteriocins / Q000494:pharmacology*; D002460:Cell Line; D002522:Chlorocebus aethiops; D014709:Vero Cells
D000563:Amebicides; D001426:Bacterial Proteins; D001430:Bacteriocins