Transgenically expressed rice germin-like protein1 in tobacco causes hyper-accumulation of H2O2 and reinforcement of the cell wall components.
Our recent report documented that the rice germin-like protein1 (OsGLP1), being a cell wall-associated protein involves in disease resistance in rice and possesses superoxide dismutase (SOD) activity as recognized by heterologous expression in tobacco. In the present study, the transgenic tobacco plants were analyzed further to decipher the detailed physiological and biochemical functions of the OsGLP1 and its associated SOD activity. The transgenic tobacco lines expressing SOD-active OsGLP1 showed tolerance against biotic and abiotic stresses mitigated by hyper-accumulating H(2)O(2) upon infection by fungal pathogen (Fusarium solani) and treatment to chemical oxidizing agent (ammonium persulfate), respectively. Histological staining revealed enhanced cross-linking of the cell wall components in the stem tissues of the transgenic plants. Fourier transform infrared spectroscopy (FTIR) analysis of the biopolymer from the stem tissues of the transgenic and untransformed plants revealed differential banding pattern of the spectra corresponding to various functional groups. Our findings demonstrate that the OsGLP1 with its inherent SOD activity is responsible for hyper-accumulation of H(2)O(2) and reinforcement of the cell wall components.
Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur, India.
Joydeep
J
Banerjee
Natasha
N
Das
Prabuddha
P
Dey
Mrinal K
MK
Maiti
D002473:Cell Wall / Q000378:metabolism* / Q000648:ultrastructure; D005670:Fusarium; D006023:Glycoproteins / Q000235:genetics*; D006861:Hydrogen Peroxide / Q000378:metabolism*; D012275:Oryza / Q000235:genetics*; D018384:Oxidative Stress; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000201:enzymology* / Q000235:genetics; D017550:Spectroscopy, Fourier Transform Infrared; D013482:Superoxide Dismutase / Q000235:genetics*; D014026:Nicotiana / Q000201:enzymology* / Q000235:genetics / Q000382:microbiology
D006023:Glycoproteins; D010940:Plant Proteins; C058814:germin; D006861:Hydrogen Peroxide; D013482:Superoxide Dismutase