Urinary 8-hydroxy-2'-deoxyguanosine reflects symptomatic status and severity of systolic dysfunction in patients with chronic heart failure.
OBJECTIVE
Oxidative stress is known to play a crucial role in the pathogenesis of heart failure (HF). We investigated whether urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG), a product of oxidative DNA damage, is a clinically useful biomarker of the severity of chronic heart failure (CHF) and oxidative stress levels in failing hearts.


RESULTS
We measured 8-OHdG in the serum obtained from the coronary sinus (CS) and aortic root (Ao) in small groups of control subjects and CHF patients. We then measured urinary 8-OHdG and other biomarkers (brain natriuretic peptide, 8-isoplastane, high-sensitivity C-reactive protein, interleukin-6, and tumour necrosis factor-α) in 31 control subjects and 194 patients with CHF [left-ventricular ejection fraction (LVEF): 28.3 ± 8.1%]. Serum 8-OHdG was significantly higher in the CS than the Ao in CHF patients only. Urinary 8-OHdG was also significantly higher in CHF patients than in control subjects, and urinary 8-OHdG became higher as New York Heart Association class increased. Moreover, there was a significant correlation between urinary 8-OHdG and LVEF (r = -0.27), pulmonary capillary wedge pressure (r = 0.31), or left-ventricular end-diastolic volume index (r = 0.22). In contrast, there was poor correlation between the severity of CHF and the other neurohumoral biomarkers.


CONCLUSIONS
In HF, urinary 8-OHdG seems to reflect the level of oxidative stress and various parameters related to symptomatic status and functional severity of CHF.
Department of Medicine and Clinical Science, Yamaguchi University Graduate School of Medicine, 1-1-1 Minamikogushi, Ube, Yamaguchi 755-8505, Japan. skoba@yamaguchi-u.ac.jp
Shigeki
S
Kobayashi
Takehisa
T
Susa
Takeo
T
Tanaka
Yasuaki
Y
Wada
Shinichi
S
Okuda
Masahiro
M
Doi
Tomoko
T
Nao
Yasuhiro
Y
Yoshiga
Jutaro
J
Yamada
Takayuki
T
Okamura
Takeshi
T
Ueyama
Syuji
S
Kawamura
Masafumi
M
Yano
Masunori
M
Matsuzaki
D000080242:8-Hydroxy-2'-Deoxyguanosine; D001011:Aorta; D015415:Biomarkers / Q000097:blood / Q000652:urine; D016022:Case-Control Studies; D054326:Coronary Sinus; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000097:blood / Q000652:urine; D018450:Disease Progression; D005260:Female; D054143:Heart Failure, Systolic / Q000097:blood / Q000473:pathology / Q000652:urine*; D006801:Humans; D016014:Linear Models; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D018384:Oxidative Stress / Q000502:physiology; D011379:Prognosis; D017382:Reactive Oxygen Species; D012720:Severity of Illness Index; D013318:Stroke Volume; D016277:Ventricular Function, Left
D015415:Biomarkers; D017382:Reactive Oxygen Species; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine