Leukocyte and platelet changes following low-dose lipopolysaccharide administration in five dogs.
Effects of low-dose LPS (0.1 μg/kg i.v.) on leukocyte and platelet parameters measured using an Advia 120 hematology analyzer were investigated. Five dogs received a saline sham treatment prior to LPS, and blood was collected before and 3, 6, and 24 h post-treatment. LPS-treated dogs had mild neutrophil toxic change and increased neutrophil bands at 3 and 6 h. Compared to saline-treated controls, total leukocyte, neutrophil, and monocyte counts of LPS-treated dogs were significantly decreased at 3 h and increased at 24 h. Compared to baseline, total leukocyte counts of LPS-treated dogs were significantly decreased at 3 h and increased at 24 h. Mean platelet volume was significantly increased and mean platelet component concentration was decreased at 3 h compared to baseline. Platelet count was significantly decreased at 3 and 6 h; plateletcrit did not change significantly. High dosage is not required in order to detect LPS-mediated hematologic effects in dogs. Low-dose LPS administration causes significant changes in leukocyte and platelet indices in dogs without causing severe clinical signs or death.
Department of Pathobiology, College of Veterinary Medicine, University of Tennessee, Knoxville, TN 379996, United States. bflatlan@utk.edu
B
B
Flatland
M M
MM
Fry
C J
CJ
LeBlanc
B W
BW
Rohrbach
D000818:Animals; D001792:Blood Platelets / Q000187:drug effects*; D004283:Dog Diseases / Q000139:chemically induced* / Q000378:metabolism; D004285:Dogs; D007962:Leukocytes / Q000187:drug effects*; D008070:Lipopolysaccharides / Q000633:toxicity*; D013997:Time Factors
D008070:Lipopolysaccharides