A theoretical investigation of the photophysical consequences of major plant light-harvesting complex aggregation within the photosynthetic membrane.
Spectroscopic measurements of Arabidopsis leaves have shown that the energy-dependent component of non-photochemical quenching (NPQ), known as qE, is associated with an absorption change at 535 nm (ΔA(535)). Identical measurements on the zeaxanthin-deficient mutant npq1 reveal a similar spectroscopic signature at 525 nm (ΔA(525)). We investigated whether these red-shifts may arise from excitonic interactions among homodimers of xanthophylls, zeaxanthin, and violaxanthin, bound at the peripheral V1 binding site on adjacent light-harvesting complex II (LHCII) trimers. Estimates of the relative geometries of these pigment pairs were obtained from the structure of LHCII. The excitonic couplings of zeaxanthin and violaxanthin dimers were probed using the time-dependent density functional theory method (TD-DFT). Calculations indicated that dimers formed between zeaxanthin or violaxanthin molecules using the published LHCII structure resulted in absorption blue shifts, typical of an H-type (parallel) geometry. In contrast, if the volume of the LHCII structure was modified to reflect the change in membrane thickness that occurs upon ΔpH formation, then both zeaxanthin and violaxanthin dimers adopted a J-type (collinear) geometry, and the resulting spectral shift was to the red region. The magnitudes of these predicted red-shifts are in good agreement with the experimental magnitudes. We therefore conclude that the observed xanthophyll red-shift results from the combination of both LHCII aggregation and changes in membrane thickness during qE. ΔA(535) may therefore be considered a "marker of aggregation" between LHCII trimers upon qE formation.
Duffy
C D P
CD
School of Biological and Chemical Sciences, Queen Mary University of London, Mile End Road, London, E1 4NS, United Kingdom.
Johnson
M P
MP
Macernis
M
M
Valkunas
L
L
Barford
W
W
Ruban
A V
AV
D017360:Arabidopsis / Q000033:anatomy & histology / Q000737:chemistry; D019281:Dimerization; D045342:Light-Harvesting Protein Complexes / Q000737:chemistry*; D008958:Models, Molecular; D008962:Models, Theoretical; D010788:Photosynthesis / Q000502:physiology; D010940:Plant Proteins / Q000737:chemistry; D011487:Protein Conformation; D011789:Quantum Theory; D020524:Thylakoids / Q000737:chemistry / Q000648:ultrastructure; D024341:Xanthophylls / Q000737:chemistry*; D065146:Zeaxanthins
D045342:Light-Harvesting Protein Complexes; D010940:Plant Proteins; D024341:Xanthophylls; D065146:Zeaxanthins; C005613:violaxanthin