Vectors for glucose-dependent protein expression in Saccharomyces cerevisiae.
Based on the p426 series of expression vectors developed by Mumberg et al. (Gene 156, 119-122, 1995), we have generated a set of plasmids that allow the glucose-dependent expression of target genes in the yeast, Saccharomyces cerevisiae. The ADH1 promoter in plasmid p426-ADH1 was replaced by the 1-kb 5'-region from either of the following genes: HXK1, YGR243, HXT4 and HXT7. Expression mediated by the respective 5'-regions was monitored with EGFP, yEGFP3-CLN2pest and TurboGFP as marker genes. Fluorescence is induced 2.7-fold using the HXK1, 2.3-fold using the YGR243-, 5-fold using the HXT7- and 12.6-fold using the HXT4 5'-regions upon depletion of glucose to a concentration of <0.5 g/l.
Thierfelder
Simone
S
Institut für Genetik, Technische Universität Dresden, 01062 Dresden, Germany. simone.thierfelder@tu-dresden.de
Ostermann
Kai
K
Göbel
Andy
A
Rödel
Gerhard
G
D015966:Gene Expression Regulation, Fungal; D005822:Genetic Vectors / Q000235:genetics* / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism
D029701:Saccharomyces cerevisiae Proteins; D005947:Glucose