Activation of the neutrophil respiratory burst by plasma from periodontitis patients is mediated by pro-inflammatory cytokines.
OBJECTIVE
To determine the effect of periodontitis patients' plasma on the neutrophil oxidative burst and the role of albumin, immunoglobulins (Igs) and cytokines.


METHODS
Plasma was collected from chronic periodontitis patients (n=11) and periodontally healthy controls (n=11) and used with/without depletion of albumin and Ig or antibody neutralization of IL-8, GM-CSF or IFN-α to prime/stimulate peripheral blood neutrophils, isolated from healthy volunteers. The respiratory burst was measured by lucigenin-dependent chemiluminescence. Plasma cytokine levels were determined by ELISA.


RESULTS
Plasmas from patients were significantly more effective in both directly stimulating neutrophil superoxide production and priming for subsequent formyl-met-leu-phe (fMLP)-stimulated superoxide production than plasmas from healthy controls (p<0.05). This difference was maintained after depletion of albumin and Ig. Plasma from patients contained higher mean levels of IL-8, GM-CSF and IFN-α. Individual neutralizing antibodies against IL-8, GM-CSF or IFN-α inhibited the direct stimulatory effect of patients' plasma, whereas the ability to prime for fMLP-stimulated superoxide production was only inhibited by neutralization of IFN-α. The stimulating and priming effects of control plasma were unaffected by antibody neutralization.


CONCLUSIONS
This study demonstrates that plasma cytokines may have a role in inducing the hyperactive (IL-8, GM-CSF, IFN-α) and hyper-reactive (IFN-α) neutrophil phenotype seen in periodontitis patients.
School of Life and Health Sciences, Aston University, Birmingham, UK.
Irundika H K
IH
Dias
John B
JB
Matthews
Iain L C
IL
Chapple
Helen J
HJ
Wright
Christopher R
CR
Dunston
Helen R
HR
Griffiths
D000328:Adult; D016022:Case-Control Studies; D002630:Chemotactic Factors / Q000494:pharmacology; D055113:Chronic Periodontitis / Q000097:blood / Q000276:immunology*; D016207:Cytokines / Q000097:blood / Q000276:immunology* / Q000494:pharmacology; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000276:immunology / Q000494:pharmacology; D006801:Humans; D018836:Inflammation Mediators / Q000494:pharmacology; D016898:Interferon-alpha / Q000276:immunology / Q000494:pharmacology; D016209:Interleukin-8 / Q000276:immunology / Q000494:pharmacology; D049449:Luminescence; D008297:Male; D008875:Middle Aged; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000031:analogs & derivatives / Q000494:pharmacology; D018375:Neutrophil Activation / Q000187:drug effects / Q000276:immunology*; D010949:Plasma / Q000737:chemistry / Q000276:immunology*; D011994:Recombinant Proteins / Q000494:pharmacology; D016897:Respiratory Burst / Q000187:drug effects / Q000276:immunology*; D018709:Statistics, Nonparametric; D013268:Stimulation, Chemical
D002630:Chemotactic Factors; D016207:Cytokines; D018836:Inflammation Mediators; D016898:Interferon-alpha; D016209:Interleukin-8; D011994:Recombinant Proteins; D009240:N-Formylmethionine Leucyl-Phenylalanine; C049813:N-formylmethionyl-leucyl-phenylalanyl-lysine; D016178:Granulocyte-Macrophage Colony-Stimulating Factor