Genotyping of Brazilian Erysipelothrix spp. strains by amplified fragment length polymorphism.
One hundred and fifty-one Erysipelothrix spp. isolates from diseased and carrier swine from Brazil were identified by PCR, submitted to serotyping and analyzed by amplified fragment length polymorphism with a single enzyme (AFLP). Reference strains from Australia and the United Kingdom were also examined. The 151 strains were classified into 18 different serotypes (1a, 1b, 2a, 2b, 4, 5, 6, 7, 8, 10, 11, 12, 15, 17, 19, 21, 24 and 25), being serotype 2b the most frequent (39.7%). By associating serotyping and PCR results, it was possible to identify 146 strains as E. rhusiopathiae and five strains as E. tonsillarum. Despite the fact that for this genus AFLP did not cluster all isolates according to serotype, origin, disease or isolation data, the execution of the technique was easy and fast, demonstrating high discriminatory power. The results produced by the AFLP analysis of Erysipelothrix spp. could also support its use as a discriminatory tool for E. rhusiopathiae and E. tonsillarum species.
Coutinho
Tania Alen
TA
Laboratório de Sanidade Suína e Virologia, Faculdade de Medicina Veterinária e Zootecnia (FMVZ), Universidade de São Paulo (USP), Av. Prof. Dr. Orlando Marques de Paiva, 87, Cidade Universitária, CEP 05508 270, São Paulo/SP, Brazil. tania_alen@hotmail.com
Imada
Yumiko
Y
de Barcellos
David Emílio Santos Neves
DE
de Oliveira
Sérgio José
SJ
Moreno
Andrea Micke
AM
D054458:Amplified Fragment Length Polymorphism Analysis / Q000379:methods*; D000818:Animals; D001315:Australia; D015373:Bacterial Typing Techniques / Q000379:methods*; D001938:Brazil; D004888:Erysipelothrix / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D005838:Genotype; D012703:Serotyping; D013552:Swine; D006113:United Kingdom