[Effect of oral glutamine on intestinal barrier function in young rats with endotoxemia].
OBJECTIVE
To study the effect of glutamine on intestinal barrier function by examining the changes of plasma D-lactic levels and diamine oxidase (DAO) levels in plasma and intestinal tissue after glutamine intervention in young rats with endotoxemia.


METHODS
Eighty 18-day-old rats were randomly divided into endotoxemia and glutamine intervention groups (n=40 each). Endotoxemia was induced by lipopolysaccharide (LPS) injection. Plasma and small intestine homogenate were collected 1.5, 6, 24 and 72 hrs and 7 days after LPS injection. The glutamine intervention group was immediately administered with oral glutamine (2 g/kg) after LPS injection. Afterwards, glutamine was administered once daily. Plasma D-lactic and DAO levels and intestinal DAO levels were measured.


RESULTS
Plasma DAO activity in the glutamine intervention group was significantly lower than that in the endotoxemia group 6 and 72 hrs after LPS injection (P<0.05). In contrast, the intestinal DAO activity in the glutamine intervention group was significantly higher than that in the endotoxemia group 6, 24 and 72 hrs and 7 days after LPS injection (P<0.05 or 0.01). Plasma D-lactic levels in the glutamine intervention group were significantly lower than those in the endotoxemia group 6, 24 and 72 hrs and 7 days after LPS injection (P<0.01).


CONCLUSIONS
Glutamine may reduce the permeability of intestinal mucosa, and thus provides protective effects on intestinal barrier function in rats with endotoxemia.
Chang
Xiao
X
Department of Neonatology, First Affiliated Hospital of Xinxiang Medical University, Weihui, Henan, China.
Wang
Lin-Lin
LL
Lian
Shu-Jun
SJ
Tang
Qing
Q
Chen
Ping
P
Wang
Hua
H
D000284:Administration, Oral; D006631:Amine Oxidase (Copper-Containing) / Q000378:metabolism; D000818:Animals; D001522:Behavior, Animal; D019446:Endotoxemia / Q000378:metabolism*; D005260:Female; D005973:Glutamine / Q000008:administration & dosage / Q000494:pharmacology*; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000187:drug effects*; D019344:Lactic Acid / Q000097:blood; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D005973:Glutamine; D019344:Lactic Acid; D006631:Amine Oxidase (Copper-Containing)