Surface grafting of a thermoplastic polyurethane with methacrylic acid by previous plasma surface activation and by ultraviolet irradiation to reduce cell adhesion.
The material performance, in a biological environment, is mainly mediated by its surface properties and by the combination of chemical, physical, biological, and mechanical properties required, for a specific application. In this study, the surface of a thermoplastic polyurethane (TPU) material (Elastollan(®)1180A50) was activated either by plasma or by ultra-violet (UV) irradiation. After surface activation, methacrylic acid (MAA) was linked to the surface of TPU in order to improve its reactivity and to reduce cell adhesion. Grafted surfaces were evaluated by X-ray photoelectron spectroscopy (XPS), by atomic force microscopy (AFM) and by contact angle measurements. Blood compatibility studies and cell adhesion tests with human bone marrow cells (HBMC) were also performed. If was found that UV grafting method led to better results than the plasma activation method, since cell adhesion was reduced when methacrylic acid was grafted to the TPU surface by UV.
Alves
P
P
Department of Chemical Engineering, University of Coimbra, Rua Sílvio Lima, Polo II, Pinhal de Marrocos, 3030-790, Coimbra, Portugal. palves@eq.uc.pt
Pinto
S
S
Kaiser
Jean-Pierre
JP
Bruinink
Arie
A
de Sousa
Hermínio C
HC
Gil
M H
MH
D001854:Bone Marrow Cells / Q000166:cytology; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000295:instrumentation; D002478:Cells, Cultured; D003470:Culture Media / Q000737:chemistry; D006461:Hemolysis; D006358:Hot Temperature; D006801:Humans; D008422:Materials Testing; D008689:Methacrylates / Q000737:chemistry*; D056951:Photoelectron Spectroscopy / Q000379:methods; D011140:Polyurethanes / Q000737:chemistry*; D013499:Surface Properties; D013997:Time Factors; D014466:Ultraviolet Rays
D003470:Culture Media; D008689:Methacrylates; D011140:Polyurethanes; C557283:elastollan 1180A50; C008384:methacrylic acid