[Cellular aspects of regulation of nuclear factor kappaB (NF-kappabeta) transcriptional activity in sensory neurons in vitro].
Nuclear factor kappaB (NF-kappaB) controls the expression of multiple anti/proapoptotic genes, including those in the nervous cells. Intranuclear deacetylation and acetylation events are implicated in the regulation of NF-kappaB transcriptional activity. The goal of the work was to demonstrate the transcriptional activity of NF-kappaB in the sensory neurons in vitro and to find out whether NF-kappaB activity could be controlled by modification of acetylation processes, using a transgenic line of NF-kappaB reporter mice in which activation of NF-kappaB drives the expression of the lac-z gene. We show that the expression of the reporter gene was absent in transgenic neurons cultured in normal growth medium or stimulated by TNF-alpha. However, NF-kappaB transcriptional activity in most neurons was induced by in vitro exposure to Trichostatin A, which is a specific inhibitor of histone deacetylases. Taken together our results show that the transcriptional activity of NF-kappaB is limited in sensory neurons due to a transcriptional repression mechanism mediated by histone deacetylases.
Gushchina
S V
SV
Volkova
O V
OV
Krugliakov
P P
PP
Magoulas
K B
KB
D000107:Acetylation / Q000187:drug effects; D000818:Animals; D002478:Cells, Cultured; D056572:Histone Deacetylase Inhibitors / Q000494:pharmacology; D006655:Histone Deacetylases / Q000235:genetics / Q000378:metabolism*; D006877:Hydroxamic Acids / Q000494:pharmacology; D051379:Mice; D008822:Mice, Transgenic; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism*; D011984:Sensory Receptor Cells / Q000166:cytology / Q000378:metabolism*; D014158:Transcription, Genetic / Q000187:drug effects / Q000502:physiology*; D001616:beta-Galactosidase / Q000096:biosynthesis* / Q000235:genetics
D056572:Histone Deacetylase Inhibitors; D006877:Hydroxamic Acids; D016328:NF-kappa B; C012589:trichostatin A; D001616:beta-Galactosidase; D006655:Histone Deacetylases