Enzyme-assisted binary probe for sensitive detection of RNA and DNA.
The new enzyme-assisted assay for DNA/RNA detection provides real-time fluorescent signal readout along with low limit of detection and high discrimination power toward a single-base substitution. Requiring only two new unmodified DNA oligonucleotides for the detection of each new analyte, the assay is an efficient tool for low-cost analysis of multiple analytes.
Chemistry Department, University of Central Florida, Orlando, FL 32816, USA. ygerasim@mail.ucf.edu
Yulia V
YV
Gerasimova
Sadia
S
Peck
Dmitry M
DM
Kolpashchikov
D055162:Biocatalysis; D004247:DNA / Q000032:analysis*; D015335:Molecular Probes / Q000737:chemistry*; D012313:RNA / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016914:Ribonuclease H / Q000737:chemistry / Q000378:metabolism*
D015335:Molecular Probes; D012313:RNA; D004247:DNA; C091139:ribonuclease HII; D016914:Ribonuclease H