Transplanted mesenchymal stem cells can inhibit the three developmental stages of murine acute graft-versus-host disease.
BACKGROUND
Recent data have demonstrated that mesenchymal stem cells (MSCs) have potent immune regulation capacity in vitro, enhancing their therapeutic appeal for their utilisation in the management of acute graft-versus-host disease (aGvHD). However, their immunoregulatory activity in vivo is largely unknown.


METHODS
Using murine compact bone-derived MSCs in an aGvHD model, the phenotypic status of splenocytes of aGvHD and aGvHD+MSC groups of mice were examined by flow cytometry.


RESULTS
MSC infusion decreased the expression of MHC-II and CD69 molecules on splenic CD11b+ cells of aGvHD mice, which resulted in decreased maturation of antigen-presenting cells. Moreover, the down-regulated ratio of CD3+CD69+ to CD3+ cells, leading to restrained early activation and effector T-cell formation, resulted in the enhancement of the absolute and relative number of splenic CD3+ cells by MSCs co-transfer.


CONCLUSIONS
This study demonstrated that MSCs can inhibit the three developmental stages of aGvHD.
Department of Cell Biology, Institute of Basic Medical Sciences, 27 Tai-Ping Road, Beijing 100850, P.R.China.
Hong
H
Li
Zikuan
Z
Guo
Heng
H
Zhu
Xiu Sen
XS
Li
Xiaoxia
X
Jiang
Huiyu
H
Yao
Xiaoyan
X
Wang
Li
L
Liao
Yuanlin
Y
Liu
Ying
Y
Wu
Yi
Y
Zhang
Ning
N
Mao
D000208:Acute Disease; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D000945:Antigens, Differentiation, T-Lymphocyte / Q000378:metabolism; D039481:CD11b Antigen / Q000378:metabolism; D017252:CD3 Complex / Q000378:metabolism; D002478:Cells, Cultured; D004195:Disease Models, Animal; D005260:Female; D005434:Flow Cytometry; D006086:Graft vs Host Disease / Q000276:immunology / Q000628:therapy*; D016130:Immunophenotyping; D037181:Lectins, C-Type / Q000378:metabolism; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D000099192:CD69 Antigens
D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D039481:CD11b Antigen; D017252:CD3 Complex; D037181:Lectins, C-Type; D000099192:CD69 Antigens