Isolation of neurocysticercosis-related antigens from a genomic phage display library of Taenia solium.
In this study, the authors have generated a tapeworm Taenia solium genomic DNA expression library where foreign peptides/proteins were fused to N-termini of M13 cpVIII and expressed at a high copy number on the phage surface, and they showed that this library may be used in bioselection against antipathogen immune sera, allowing the identification of disease-related antigens recognizing antibodies present in clinical samples. They isolated 2 phage clones expressing T. solium-derived antigens specifically reacting with antibodies present in plasma and cerebrospinal fluid samples of neuroimaging-confirmed neurocysticercosis patients. The described antigen discovery strategy may be used for the direct identification of antigens useful for host-pathogen interaction studies as well as for the development of molecular vaccines and diagnostics.
González
Erik
E
Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, México DF, México.
Robles
Yetzi
Y
Govezensky
Tzipe
T
Bobes
Raul J
RJ
Gevorkian
Goar
G
Manoutcharian
Karen
K
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood* / Q000134:cerebrospinal fluid* / Q000235:genetics / Q000276:immunology*; D000947:Antigens, Helminth / Q000235:genetics / Q000276:immunology; D017104:Bacteriophage M13 / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015698:Genomic Library; D006790:Host-Parasite Interactions; D006801:Humans; D007074:Immunoglobulin G / Q000134:cerebrospinal fluid / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D020019:Neurocysticercosis / Q000175:diagnosis / Q000276:immunology*; D019151:Peptide Library; D041201:Taenia solium / Q000235:genetics / Q000276:immunology*
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D007074:Immunoglobulin G; D019151:Peptide Library