False-negative PCR result due to gene polymorphism: the example of Neisseria meningitidis.
Early treatment of meningococcal meningitis is mandatory but may negate the cerebrospinal fluid culture. Etiological diagnosis then mainly relies on PCR. Here, we report a case of false-negative results for real-time PCR for a Neisseria meningitidis serogroup B isolate with a polymorphism in the ctrA gene.
Institute of Microbiology, University Hospital of Lausanne (CHUV), Bugnon 48, 1011 Lausanne, Switzerland. Katia.Jaton-Ogay@chuv.ch
Katia
K
Jaton
Béatrice
B
Ninet
Jacques
J
Bille
Gilbert
G
Greub
D001426:Bacterial Proteins / Q000235:genetics; D001431:Bacteriological Techniques / Q000379:methods*; D002675:Child, Preschool; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005188:False Negative Reactions; D006801:Humans; D008297:Male; D008589:Meningococcal Infections / Q000175:diagnosis / Q000382:microbiology*; D008969:Molecular Sequence Data; D009345:Neisseria meningitidis / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA
D001426:Bacterial Proteins; D004269:DNA, Bacterial