Antibiotic manipulation of intestinal microbiota to identify microbes associated with Campylobacter jejuni exclusion in poultry.
The ability of various subsets of poultry intestinal microbiota to protect turkeys from colonization by Campylobacter jejuni was investigated. Community subsets were generated in vivo by inoculation of day-old poults with the cecal contents of a Campylobacter-free adult turkey, followed by treatment with one antimicrobial, either virginiamycin, enrofloxacin, neomycin, or vancomycin. The C. jejuni loads of the enrofloxacin-, neomycin-, and vancomycin-derived communities were decreased by 1 log, 2 logs, and 4 logs, respectively. Examination of the constituents of the derived communities via the array-based method oligonucleotide fingerprinting of rRNA genes detected a subtype of Megamonas hypermegale specific to the C. jejuni-suppressive treatments.
Scupham
A J
AJ
Pre-Harvest Food Safety and Enteric Diseases Research Unit, National Animal Disease Center, USDA, Agricultural Research Service, Ames, Iowa 50010, USA. alexandra.scupham@aphis.usda.gov
Jones
J A
JA
Rettedal
E A
EA
Weber
T E
TE
D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage*; D000898:Antibiosis; D058491:Bacterial Load; D002169:Campylobacter Infections / Q000517:prevention & control / Q000662:veterinary*; D016123:Campylobacter jejuni / Q000254:growth & development*; D002353:Carrier State / Q000517:prevention & control / Q000662:veterinary*; D002432:Cecum / Q000382:microbiology*; D054892:Metagenome / Q000187:drug effects; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D014422:Turkeys; D045854:Veillonellaceae / Q000502:physiology*
D000900:Anti-Bacterial Agents