BLOS1, a putative BLOC-1 subunit, interacts with SNX1 and modulates root growth in Arabidopsis.
Internalization and sorting of macromolecules are inherent properties of all eukaryotic cells that are achieved by vesicle trafficking. However, this process is relatively less understood in plants. An eight-subunit protein complex, BLOC-1, which is involved in endosomal transport from the endosomes to the lysosomes, has been identified in both human and mice. In this study, two homologous subunits of this complex, BLOS1 (or AtGCN5L1) and BLOS2, have been characterized in Arabidopsis. Both BLOS1 and BLOS2 interacted with SNX1 on the sorting endosomes. Inducible RNAi lines with reduced levels of BLOS1 had longer primary roots and more lateral roots. Consistently, PIN1 and PIN2 were increased in BLOS1 RNAi lines, implicating an impaired transport from the endosomes to the vacuoles. These results suggest that a putative BLOC-1 complex in Arabidopsis might mediate the vacuolar degradative transport through direct interaction with SNX1 to regulate the homeostasis of PIN1 and PIN2, which is important for plant growth and development.
Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
Yanyan
Y
Cui
Xugang
X
Li
Qingguo
Q
Chen
Xin
X
He
Qing
Q
Yang
Aili
A
Zhang
Xin
X
Yu
Hao
H
Chen
Naiyou
N
Liu
Qi
Q
Xie
Weicai
W
Yang
Jianru
J
Zuo
Klaus
K
Palme
Wei
W
Li
D000595:Amino Acid Sequence; D000818:Animals; D017360:Arabidopsis / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000502:physiology; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D048708:Cell Growth Processes / Q000235:genetics; D011992:Endosomes / Q000378:metabolism; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D037102:Lectins / Q000235:genetics / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000502:physiology; D051379:Mice; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000235:genetics / Q000254:growth & development; D011485:Protein Binding / Q000235:genetics; D021381:Protein Transport / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D058305:Sorting Nexins / Q000378:metabolism; D020798:Two-Hybrid System Techniques; D015003:Yeasts / Q000235:genetics*
D029681:Arabidopsis Proteins; D002352:Carrier Proteins; D047908:Intracellular Signaling Peptides and Proteins; D037102:Lectins; D026901:Membrane Transport Proteins; D009419:Nerve Tissue Proteins; D010940:Plant Proteins; D058305:Sorting Nexins; C104378:BLOC1S1 protein, human; C513476:Bloc1s6 protein, mouse; C116363:PIN1 protein, Arabidopsis; C117013:AT5G57090 protein, Arabidopsis