Evaluation of a liquid bead array system for high-risk human papillomavirus detection and genotyping in comparison with Hybrid Capture II, DNA chip and sequencing methods.
Since persistent infection with high-risk human papillomavirus (HPV) is a known cause of high-grade cervical intraepithelial neoplasia and cervical cancer, several HPV DNA detection methods have been developed during the last decade. The Hybrid Capture II (HCII) assay, which allows detection of 13 high-risk HPVs, has been well validated; however, it does not provide any genotype-specific information. The oncogenic activity of HPV is dependent on its genotype. The prophylactic effects of HPV vaccines are based on L1 virus-like particles and are limited mainly to infections corresponding to the HPV type used to develop the immunogen. Therefore, accurate detection and genotyping are important for treatment as well as screening. A newly developed HPV genotyping system using a liquid bead array was evaluated with 286 cervical samples and the results were compared to two commercially available methods, i.e. the HCII and HPV DNA chip assays, and sequencing. The sensitivity for detection of high-risk HPV was 85.3 % (HCII), 94.7 % (DNA chip) and 99.0 % (liquid bead array). The liquid bead array showed almost perfect agreement (κ=0.95) with genotype information confirmed by sequencing, while substantial agreement (κ=0.8) was observed between DNA chip and sequencing. Furthermore, the liquid bead array had superior detection of 26 HPVs (16 high-risk and 10 low-risk types) and has proven to be as accurate as sequencing in identifying individual HPV types, even in cases with multiple HPV infections.
Department of Anesthesiology, Catholic University of Korea, 505 Banpodong, Seocho-ku, Seoul 137-040, Republic of Korea.
Eun Jung
EJ
Cho
Cancer Research Institute of Medical Science, Catholic University of Korea, 505 Banpodong, Seocho-ku, Seoul 137-040, Republic of Korea.
Jin Hwan
JH
Do
Cancer Research Institute of Medical Science, Catholic University of Korea, 505 Banpodong, Seocho-ku, Seoul 137-040, Republic of Korea.
Yoon Sun
YS
Kim
Cancer Research Institute of Medical Science, Catholic University of Korea, 505 Banpodong, Seocho-ku, Seoul 137-040, Republic of Korea.
Sumi
S
Bae
Department of Obstetrics and Gynecology, Catholic University of Korea, 505 Banpodong, Seocho-ku, Seoul 137-040, Republic of Korea.
Woong Shick
WS
Ahn
D005260:Female; D006801:Humans; D046228:Microarray Analysis / Q000379:methods; D008863:Microspheres; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D027383:Papillomaviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000175:diagnosis* / Q000821:virology*; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D014773:Virology / Q000379:methods*