uPA, uPAR and TGFβ₁ expression during early and late post myocardial infarction period in rat myocardium.
The expression patterns of transforming growth factor beta 1 (TGFβ₁), urokinase-type plasminogen activator (uPA) and uPA receptor (uPAR) were analysed after artery ligation-induced myocardial infarction (MI) in the rat myocardium. uPA and uPAR expressions were significantly increased both at transcriptional and protein level during early phase post MI period (uPA at 1 hour and uPAR at 24 hours post infarction). TGFβ1 mRNA expression profile revealed a significant increase of TGFβ1 expression from day 4 up to 8 weeks post infarction. These data suggest that the need for an increasing TGFβ₁ bioavailability during the post-infarction period in rat myocardium is achieved in the early post MI period by an increased expression of uPA/uPAR proteolytic system (indirect activation of latent TGFβ₁) and in the late post MI period by direct regulation of TGFβ₁ expression. It is therefore concluded that differential regulation of the TGFβ₁ bioavailability may be a crucial step of the repair mechanisms during the post MI infarction period in the rat myocardium.
Department of Experimental Physiology, Medical School, National & Kapodistrian University of Athens, 75 Micras Asias, Goudi-Athens 11527, Greece.
Anastasia
A
Stavropoulou
Anastassios
A
Philippou
Antonios
A
Halapas
Antigone
A
Sourla
Nikolaos
N
Pissimissis
Michael
M
Koutsilieris
D000818:Animals; D005109:Extracellular Matrix / Q000502:physiology; D015870:Gene Expression / Q000502:physiology; D006321:Heart / Q000502:physiology*; D008297:Male; D009203:Myocardial Infarction / Q000235:genetics / Q000473:pathology / Q000503:physiopathology*; D009206:Myocardium / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D055293:Receptors, Urokinase Plasminogen Activator / Q000235:genetics*; D053773:Transforming Growth Factor beta1 / Q000235:genetics*; D014568:Urokinase-Type Plasminogen Activator / Q000235:genetics*; D020257:Ventricular Remodeling / Q000502:physiology
D055293:Receptors, Urokinase Plasminogen Activator; D053773:Transforming Growth Factor beta1; D014568:Urokinase-Type Plasminogen Activator