D-chiro-inositol affects accumulation of raffinose family oligosaccharides in developing embryos of Pisum sativum.
Developing garden pea embryos are able to take up exogenously applied cyclitols: myo-inositol, which naturally occurs in pea, and two cyclitols absent in pea plants: d-chiro-inositol and d-pinitol. The competition in the uptake of cyclitols by pea embryo, insensitivity to glucose and sucrose, and susceptibility to inhibitor(s) of H(+)-symporters (e.g. CCCP and antimycin A) suggest that a common cyclitol transporter is involved. Both d-chiro-inositol and d-pinitol can be translocated through the pea plant to developing embryos. During seed maturation drying, they are used for synthesis of mainly mono-galactosides, such as fagopyritol B1 and galactosyl pinitol A. Accumulation of d-chiro-inositol (and formation of fagopyritols), but not d-pinitol, strongly reduces accumulation of verbascose, the main raffinose oligosaccharide in pea seeds. The reasons for the observed changes are discussed.
Department of Plant Physiology and Biotechnology, University of Warmia and Mazury in Olsztyn, Olsztyn, Oczapowskiego, Poland. lahuta@uwm.edu.pl
Lesław B
LB
Lahuta
Tomasz
T
Dzik
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000968:Antimycin A / Q000494:pharmacology; D001692:Biological Transport / Q000187:drug effects; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology; D004187:Disaccharides / Q000032:analysis / Q000378:metabolism; D007294:Inositol / Q000031:analogs & derivatives / Q000032:analysis / Q000494:pharmacology*; D009844:Oligosaccharides / Q000032:analysis / Q000378:metabolism*; D018532:Pisum sativum / Q000187:drug effects / Q000196:embryology / Q000378:metabolism*; D011887:Raffinose / Q000032:analysis / Q000378:metabolism; D012639:Seeds / Q000187:drug effects / Q000378:metabolism*; D013997:Time Factors; D014475:Uncoupling Agents / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D004187:Disaccharides; D009844:Oligosaccharides; D014475:Uncoupling Agents; C112483:fagopyritol B1; C005695:stachyose; C021730:pinitol; D007294:Inositol; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D000968:Antimycin A; D011887:Raffinose