Determination of ethyl glucuronide in hair samples of Chinese people by protein precipitation (PPT) and large volume injection-gas chromatography-tandem mass spectrometry (LVI-GC/MS/MS).
Ethyl glucuronide (EtG) has been shown to be a suitable marker of excessive alcohol consumption. Determination of EtG in hair samples may help to differentiate social drinkers from alcoholics, and this testing can be widely used in forensic science, treatment programs, workplaces, military bases as well as driving ability test to provide legal proof of drinking. A method for determination of EtG in hair samples using large volume injection-gas chromatography-tandem mass spectrometry (LVI-GC/MS/MS) was developed and validated. Hair samples (in 1 mL deionized water) were ultrasonicated for 1h and incubated overnight; these samples were then deproteinated to remove impurities and derivatisated with 15 μL of pyridine and 30 μL of BSTFA. EtG was detected using GC/MS/MS in multiple-reaction monitoring mode. This method exhibited good linearity: y=0.0036 x+0.0437, R²=0.9993, the limit of detection and the limit of quantification were 5 pg/mg and 10 pg/mg, respectively. The extraction recoveries were more than 60%, and the inter-day and intra-day relative standard deviations (RSD) were less than 15%. This method has been applied to the analysis of EtG in hair samples from 21 Chinese subjects. The results for samples obtained from all of those who were teetotallers were negative, and the results for the other 15 samples ranged from 10 to 78 pg/mg, except for one negative sample. These data are the basis for interpretation of alcohol abuse.
Shi
Yan
Y
Department of Forensic Science, Shanghai Medical College, Fudan University, Shanghai 200032, PR China.
Shen
Baohua
B
Xiang
Ping
P
Yan
Hui
H
Shen
Min
M
D000293:Adolescent; D000437:Alcoholism / Q000175:diagnosis / Q000378:metabolism; D011232:Chemical Precipitation; D002648:Child; D002681:China; D000431:Ethanol / Q000378:metabolism; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D005965:Glucuronates / Q000032:analysis* / Q000378:metabolism; D006197:Hair / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D008297:Male; D055815:Young Adult
D005965:Glucuronates; C093924:ethyl glucuronide; D000431:Ethanol