Enhanced differentiation of embryonic and neural stem cells to neuronal fates on laminin peptides doped polypyrrole.
PPy is a conducting polymer material that has been widely investigated for biomedical applications. hESCs and adult rNSCs were grown on four PPy surfaces doped with PSS or peptide from laminin (p20, p31, and a mixture of p20 and p31) respectively. After 7 d, both PPy/p20 and PPy/p31 promoted neuroectoderm formation from hESCs. After 14 d of culture, surfaces containing p20 showed the highest percentage of neuronal differentiation from hESC, while the PPy/p31 surface showed better cell attachment and spreading. In rNSCs cultures, a higher percentage of neurons were found on the PPy/p20 surface than other surfaces at 7 and 14 d. For differentiated neurons, p20 promoted both the primary and total neurite outgrowth. Longer primary neurites were found on p20-containing surfaces and a longer total neurite length was found on PPy/p20 surface. These results demonstrated that, by doping PPy with different bioactive peptides, differentiation of stem cells seeded at different stages of development is affected.
Zhang
Ling
L
Department of Bioengineering, McGowan Institute for Regenerative Medicine, University of Pittsburgh, 5065 BioScience Tower 3, 3501 Fifth Ave, Pittsburgh, Pennsylvania 15260, USA.
Stauffer
William R
WR
Jane
Esther P
EP
Sammak
Paul J
PJ
Cui
Xinyan Tracy
XT
D000596:Amino Acids / Q000032:analysis; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology*; D004563:Electrochemistry; D053595:Embryonic Stem Cells / Q000166:cytology*; D006801:Humans; D007797:Laminin / Q000737:chemistry* / Q000494:pharmacology; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D058953:Neural Stem Cells / Q000166:cytology*; D009474:Neurons / Q000166:cytology*; D058105:Polymerization; D011108:Polymers / Q000737:chemistry* / Q000494:pharmacology; D011758:Pyrroles / Q000737:chemistry* / Q000494:pharmacology; D013997:Time Factors; D023822:Tissue Engineering / Q000379:methods*
D000596:Amino Acids; D007797:Laminin; D011108:Polymers; D011758:Pyrroles; C067635:polypyrrole