Qualitative and quantitative analysis of retinol, retinyl esters, tocopherols and selected carotenoids out of various internal organs form different species by HPLC.
We report the validation of a reversed-phase gradient HPLC method allowing simultaneous quantification of retinol, retinyl esters, tocopherols and selected carotenoids in lung, liver and plasma of mouse, rat and guinea pig (gp) using a diode array detector. A significant species difference was observed regarding the distribution of retinol and retinyl esters. The levels of total retinol in lung, liver and plasma were in the following order: mouse >> rat > gp; rat >mouse > gp; and gp >> rat > mouse, respectively. Furthermore, comparison studies revealed similarities between the vitamin A profiles of human and gp lung samples.
Meharry Medical College, Dept. of Biochemistry and Cancer Biology, 1005, Dr. D.B. Todd, Jr. Blvd., Nashville, TN 37208, U.S.A.
Michael W
MW
Schäffer
Somdutta Sinha
SS
Roy
Shyamali
S
Mukherjee
Donatus
D
Nohr
Michael
M
Wolter
Hans K
HK
Biesalski
David E
DE
Ong
Salil K
SK
Das