Coating electrospun chitosan nanofibers with polyelectrolyte multilayers using the polysaccharides heparin and N,N,N-trimethyl chitosan.
A new method is presented for functionalizing electrospun nanofibers with GAGs and growth factors by PEM deposition. Electrospun chitosan nanofibers, spun from trifluoroacetic acid and dichloromethane, were coated with PEMs, using the polysaccharides heparin and N,N,N-trimethyl chitosan. FGF-2 was adsorbed on the PEM-coated nanofibers. Nanofiber neutralization, PEM construction, and FGF-2 adsorption were monitored using FT-IR spectroscopy and X-ray photoelectron spectroscopy. Alcian blue staining was used to confirm the presence of heparin. SEM was used to study nanofiber morphology.
Department of Chemical and Biological Engineering, 1370 Campus Delivery, Colorado State University, Fort Collins, Colorado 80523-1370, USA.
Jorge
J
Almodóvar
Matt J
MJ
Kipper
D048271:Chitosan / Q000737:chemistry*; D055664:Electrochemical Techniques; D016222:Fibroblast Growth Factor 2 / Q000737:chemistry*; D006493:Heparin / Q000737:chemistry*; D057139:Nanofibers / Q000737:chemistry*; D056951:Photoelectron Spectroscopy; D011485:Protein Binding; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties
C118071:N-trimethyl chitosan chloride; D016222:Fibroblast Growth Factor 2; D006493:Heparin; D048271:Chitosan