[Cloning of full-length coding sequence of tree shrew CD3E and prediction of its molecular characteristics].
The use of tree shrews (Tupaia belangeri) in human disease studies demands essential research tools, in particular cellular markers and their monoclonal antibodies for immunological studies. Here we cloned the full-length cDNAs encoding CD3E from total RNA of the spleen, liver and peripheral blood of tree shrews and analyzed their structural characteristics in comparison with other mammals by Discovery Studio software. The results showed that the open reading frame sequence of tree shrew CD3E was 582 bp, encoding 194 amino acids. The overall structure of tree shrew CD3E protein was similar to its counterparts of other mammals, intracellular and transmembrane domain highly conserved. However, detailed analysis revealed two potential glycosylation sites and different surface charges in the extracellular domain. Availability of the entire open-reading-frame and related sequence information would therefore facilitate the preparation of monoclonal antibodies against tree shrew CD3 and further studies for its function.
College of Animal Science and Veterinary Medicine, Yunnan Agricultural University, Kunming Yunnan, China. liyijiang88.student@sina.com
Yi-Jiang
YJ
Li
Yue-Dong
YD
Gao
Yan
Y
Guo
Cai-Xia
CX
Lu
Jing-Fei
JF
Huang
Xue-Shan
XS
Xia
Jie-Jie
JJ
Dai
Quan-Shui
QS
Fan
Zuo-Sheng
ZS
Li
Hua-Tang
HT
Zhang
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017252:CD3 Complex / Q000737:chemistry / Q000235:genetics*; D003001:Cloning, Molecular; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010802:Phylogeny; D014418:Tupaiidae / Q000276:immunology*
D017252:CD3 Complex