Different expression patterns of pAkt, NF-κB and cyclin D1 proteins during the invasion process of head and neck squamous cell carcinoma: an in vitro approach.
BACKGROUND
Several signaling pathways are involved in the progression of squamous cell carcinoma. Among them, activated PI3K/Akt may result in NF-κB nuclear translocation, thus leading to the transcription of genes enrolled in cellular invasion and proliferation, such as cyclin D1. This study sought to evaluate the expression of pAkt, NF-κB and cyclin D1 proteins in head and neck squamous cell carcinoma cell lines and their respective in vitro-obtained invasive clones.


METHODS
Squamous cell carcinoma cell lines originating from the tongue, pharynx and the metastatic lymph node were submitted to an in vitro invasion assay to select invasive clones. All experimental groups were submitted to immunofluorescence and Western blot assays. Statistical analysis was performed through a Student's t-test with a significance level of 5%.


RESULTS
The pAkt and NF-κB expression differed from cytoplasm and nucleus depending on the studied cell line. The invasive clone from the tongue presented a network-like structure of pAkt's cytoplasmic expression. This lineage as well as the invasive clone from pharynx also showed pAkt and NF-κB nuclear transportation. Significant pAkt and NF-κB increases were observed in the tongue and pharynx invasive clones. Cyclin D1 was detected in the nucleus of all studied cells and was significantly enhanced in the invasive clones from tongue and pharynx.


CONCLUSIONS
This study suggests the participation of pAkt, NF-κB and cyclin D1 in the invasion process of head and neck squamous cell carcinoma. Moreover, cytoplasmic pAkt network-like structure was probably related to cytoskeleton changes presented during invasion.
Department of Oral Pathology, School of Dentistry, University of São Paulo, São Paulo, Brazil. fernandagiudice@usp.br
Fernanda Salgueiredo
FS
Giudice
Aluana Maria da Costa
AM
Dal Vechio
Aline Corrêa
AC
Abrahão
Felipe Fornias
FF
Sperandio
Décio dos Santos
Ddos S
Pinto-Junior
D021581:Active Transport, Cell Nucleus; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D002999:Clone Cells; D019938:Cyclin D1 / Q000096:biosynthesis*; D003599:Cytoskeleton / Q000378:metabolism; D058750:Epithelial-Mesenchymal Transition; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D016328:NF-kappa B / Q000096:biosynthesis*; D009361:Neoplasm Invasiveness / Q000235:genetics*; D010610:Pharyngeal Neoplasms / Q000235:genetics / Q000378:metabolism*; D051057:Proto-Oncogene Proteins c-akt / Q000096:biosynthesis*; D015398:Signal Transduction; D014062:Tongue Neoplasms / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation
D016328:NF-kappa B; D019938:Cyclin D1; D051057:Proto-Oncogene Proteins c-akt