[Application of direct amplification with PowerPlex 16 HS system for detection of DNA in conventional evidence materials].
OBJECTIVE
To study the effectiveness of direct amplification with PowerPlex 16 HS system for DNA detection in three conventional materials: fresh blood, oral swab, and cigarette butt.


METHODS
The genetic data of 11 samples of fresh blood, 10 samples of oral swab and 10 samples of cigarette butts were analyzed with PowerPlex 16 HS kit. The result was statistically analyzed.


RESULTS
Success rate of whole genetic map test in fresh blood and oral swab samples was 100%. Success rate in cigarette butt samples was 90%.


CONCLUSIONS
Direct amplification with PowerPlex 16 HS system is effective method to three types of conventional evidence material including fresh blood, oral swab and cigarette butt.
Criminal Police Branch, Wuhu Public Security Bureau, Wuhu 241000, China. protease168@yahoo.com.cn
Mi
M
Zhou
Tao
T
Tang
Fan
F
Wei
Han-qiu
HQ
Zhang
Xin-xin
XX
Li
Ren-jie
RJ
Ding
Jin
J
Zhou
Cheng-quan
CQ
Wang
D001801:Blood Stains; D004247:DNA / Q000032:analysis* / Q000235:genetics; D016172:DNA Fingerprinting / Q000379:methods*; D053803:Forensic Genetics / Q000379:methods; D006801:Humans; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods; D012463:Saliva / Q000378:metabolism; D012680:Sensitivity and Specificity; D020080:Tandem Repeat Sequences; D014026:Nicotiana
D004247:DNA