Structures of 2-acetylaminofluorene modified DNA revisited: insight into conformational heterogeneity.
Despite the extensive data on dG-AAF, the major DNA adduct derived from the model carcinogen 2-acetylaminofluorene, little is known with respect to its solution structures. Here, we provide NMR/CD evidence for three conformers of dG-AAF in duplex DNA: major groove B-type (B), base-displaced stacked (S), and minor groove wedge (W). The S/B/W-conformational heterogeneities were found to be sensitive to the nature of the flanking DNA sequence contexts and pH.
Department of Biomedical and Pharmaceutical Sciences, College of Pharmacy, University of Rhode Island, Kingston, Rhode Island 02881, USA.
Satyakam
S
Patnaik
Bongsup P
BP
Cho
D015073:2-Acetylaminofluorene / Q000737:chemistry*; D002273:Carcinogens / Q000737:chemistry; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry*; D018736:DNA Adducts / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D008968:Molecular Conformation; D013696:Temperature
D002273:Carcinogens; D018736:DNA Adducts; D004247:DNA; D015073:2-Acetylaminofluorene