pH-dependent localization of Btn1p in the yeast model for Batten disease.
Btn1p the yeast homolog of human CLN3, which is associated with juvenile Batten disease has been implicated in several cellular pathways. Yeast cells lacking BTN1 are unable to couple ATP hydrolysis and proton pumping activities by the vacuolar ATPase (V-ATPase). In this work, we demonstrate that changes in extracellular pH result in altered transcription of BTN1, as well as a change in the glycosylation state and localization of Btn1p. At high pH, Btn1p expression was increased and the protein was mainly located in vacuolar membranes. However, low pH decreased Btn1p expression and changed its location to undefined punctate membranes. Moreover, our results suggest that differential Btn1p localization may be regulated by its glycosylation state. Underlying pathogenic implications for Batten disease of altered cellular distribution of CLN3 are discussed.
Wolfe
Devin M
DM
Center for Neural Development and Disease, Aab Institute of Biomedical Sciences, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA.
Padilla-Lopez
Sergio
S
Vitiello
Seasson Phillips
SP
Pearce
David A
DA
D003470:Culture Media / Q000494:pharmacology; D016213:Cyclins / Q000235:genetics / Q000378:metabolism*; D005110:Extracellular Space; D015966:Gene Expression Regulation, Fungal; D006863:Hydrogen-Ion Concentration; D008954:Models, Biological; D009472:Neuronal Ceroid-Lipofuscinoses / Q000378:metabolism* / Q000473:pathology*; D011499:Protein Processing, Post-Translational; D021381:Protein Transport; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*
D003470:Culture Media; D016213:Cyclins; D012333:RNA, Messenger; D029701:Saccharomyces cerevisiae Proteins; C489809:YHC3 protein, S cerevisiae