In vivo efficacy of tamarind (Tamarindus indica) fruit extract on experimental fluoride exposure in rats.
The study was undertaken to determine the efficacy of hydro-methanolic (1:1) extract of tamarind (Tamarindus indica L.) fruit pulp in removing body fluoride burden. Thirty rats were divided into five groups. Keeping no fluoride group as the control, rats of no treatment, low dose, middle dose and high dose groups received sodium fluoride orally at the rate of 200mg per kg body weight daily for 14 weeks. Rats of low dose, middle dose and high dose group simultaneously received tamarind fruit pulp extract at three doses, viz. 25 (low), 50 (medium) and 100mg (high) per kg body weight orally, respectively. Fluoride concentration in blood, urine and long bone of experimental rats was monitored to assess the efficacy of the extract. Mean serum fluoride concentration in fluoride exposed rats was 0.145 ± 0.009 and 0.783 ± 0.042 μg/ml on days 0 and 98. In comparison, fluoride concentrations in tamarind treated rats were 0.179 ± 0.021 and 0.633 ± 0.015; 0.179 ± 0.021 and 0.502 ± 0.025 and 0.176 ± 0.021 and 0.498 ± 0.030 μg/ml in low, medium and high dose groups, respectively on day 0 and day 98 of the experiment. There was a significant (p ≤ 0.01) increase in urinary fluoride excretion from day 28 onwards. The mean fluoride concentration in long bones of treated rats was significantly lower than the values recorded in fluoride exposed rats. These findings suggest that concomitant use of tamarind fruit pulp extract can reduce fluoride concentration in blood and bone and enhanced urinary excretion, indicating the ameliorative potential of fruits of tamarind in fluoride toxicity.
Dey
S
S
Laboratory of Comparative System of Medicine, Division of Medicine, Indian Veterinary Research Institute, Izatnagar 243 122, UP, India. sahadeb_dey@hotmail.com
Swarup
D
D
Saxena
Anju
A
Dan
Ananya
A
D000818:Animals; D000931:Antidotes / Q000737:chemistry / Q000494:pharmacology*; D001842:Bone and Bones / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D005260:Female; D005638:Fruit / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D012969:Sodium Fluoride / Q000097:blood / Q000737:chemistry / Q000633:toxicity* / Q000652:urine; D029861:Tamarindus / Q000737:chemistry*
D000931:Antidotes; D010936:Plant Extracts; D012969:Sodium Fluoride