title: An HPLC-MS/MS method for the quantitative determination of 4-hydroxy-anethole trithione in human plasma and its application to a pharmacokinetic study.
abstract: A selective, rapid and sensitive method for the quantitation of 4-hydroxy-anethole trithione (ATX) in human plasma based on high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was developed and validated. Paracetamol was used as the internal standard (I.S.). After liquid-liquid extraction of 500 μL plasma with ethyl acetate, ATX and the I.S. were chromatographed on an Inertsil(®) ODS-3 column. The mobile phase was consisted of methanol-water (75:25, v/v) with a flow rate of 0.25 mL/min. The detection was performed on a triple quadrupole tandem mass spectrometer by multiple reaction monitoring (MRM) mode via electrospray ionization (ESI) source. The calibration curve was linear over the range of 0.452-603 ng/mL (r(2)≥0.99) with a lower limit of quantitation (LLOQ) of 0.452 ng/mL. The intra- and inter-day precision (relative standard deviation, R.S.D.) values were below 13% and the accuracy (relative error, R.E.) was from -2.7% to -7.5% at three quality control levels. The assay herein described was successfully applied to a pharmacokinetic study of anethole trithione (ATT) tablet in healthy volunteers after oral administration.
authors.affiliation: Department of Analytical Chemistry, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang 110016, PR China.
authors.forename: Jifen
authors.initials: J
authors.lastname: He
authors.forename: Ting
authors.initials: T
authors.lastname: Qin
authors.forename: Jing
authors.initials: J
authors.lastname: Wen
authors.forename: Feng
authors.initials: F
authors.lastname: Qin
authors.forename: Famei
authors.initials: F
authors.lastname: Li
mesh_terms: D000781:Anethole Trithione / Q000008:administration & dosage* / Q000031:analogs & derivatives* / Q000097:blood / Q000378:metabolism / Q000493:pharmacokinetics; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D004355:Drug Stability; D006801:Humans; D008297:Male; D011786:Quality Control; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization; D013607:Tablets; D053719:Tandem Mass Spectrometry
chemical_list: C558259:4-hydroxy-anethole trithione; D013607:Tablets; D000781:Anethole Trithione