[Effect of mycophenolic acid on maturation, endocytotic capacity and allostimulatory properties of human myeloid dendritic cell].
OBJECTIVE
To study the effect of mycophenolic acid (MPA) on maturation, endocytotic capacity and allostimulatory properties of human myeloid dendritic cell (MDC).


METHODS
The peripheral blood mononuclear cells were isolated freshly from healthy volunteers (n = 15). The study group was treated by MPA. Co-stimulatory and adhesion molecular on MDC were analyzed by flow cytometry. After isolation of blood dendritic cell antigen-1(+) (BDCA-1(+)), the cellular uptake of FITC-dextran was measured by flow cytometry. After mixed lymphocyte reaction, the percentage of allogenic CD(4)(+) T cell in G(0) phase was analyzed by flow cytometry.


RESULTS
(1) Maturation: Compared to control group, MPA down-regulated the level of CD40, CD62L, HLA-DR, CD54, CD80, CD83 and CD86 on MDC significantly in study group (t = -3.713, P > 0.010). (2) Endocytotic capacity: MPA enhanced significantly the endocytotic capacity of MDC in study group (t = 10.171, P = 0.000). (3) Allostimulatory capacity: Compared to control group, MPA reduced the ability of MDC to stimulate markedly the T cell proliferation in study group.


CONCLUSIONS
MPA may enhance the phagocytic capacity of human peripheral MDC, inhibit the maturity of MDC and impair its allostimulatory capacity of CD(4)(+) T lymphocyte.
Hou
Jing
J
Department of General Surgery, Beijing Hospital, Beijing 100730, China.
Tang
Da-nian
DN
Xu
Yuan
Y
Wei
Jun-min
JM
He
Xiu-wen
XW
Li
Yong-guo
YG
D000328:Adult; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000166:cytology / Q000187:drug effects*; D005260:Female; D006801:Humans; D008297:Male; D009173:Mycophenolic Acid / Q000494:pharmacology*; D010587:Phagocytosis / Q000187:drug effects
D009173:Mycophenolic Acid