[Effect mechanism of Hcy on damage of BBB and the intervention of the compounded vitamin in rat].
OBJECTIVE
To study the effect of homocysteine (Hcy) on the damage of blood-brain barrier, and the intervention of the compounded vitamin in rat.


METHODS
54 normal male SD rats were randomly distributed into three groups: (1) Control group; (2) Model group; and (3) Intervention group. The level of plasma Hcy was detected with high performance liquid chromatography. The content of Evans Blue leaked out brain tissue was measured by colorimetry. The transcription of matrix metalloproteinase-2 (MMP-2) mRNA was evaluated by RT-PCR.


RESULTS
The level of plasma Hcy, and the content of Evans Blue leaked out brain tissue and the expression of MMP-2 mRNA of model group were 32.2 +/- 1.3, 6.67 +/- 0.54, 0.372 +/- 0.088 respectively,all significantly higher than those of the control group (12. 4 +/- 0. 9, 3.32 +/- 0.56, 0.035 +/- 0. 21, all P < 0.01). The level of plasma Hcy, and the content of Evans Blue leaked out brain tissue and the expression of MMP-2 mRNA of the intervention group was significantly lower than those of the model group (12.5 +/- 1.4 vs 32.2 +/- 1.3, 3.42 +/- 0.45 vs 6.67 +/- 0.54, 0.118 +/- 0.052 vs 0.372 +/- 0.088, P < 0.01).


CONCLUSIONS
These results indicate Hcy could increase the permeability of blood-brain barrier, the mechanism may be activating the transcription of MMP-2 in rats. The compounded vitamin has a protective effect on blood-brain barrier via lowering the level of plasma Hcy.
Peng
Hai
H
Department of Neurology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China. Penghai493@126.com
Cheng
He
H
Xing
Hong-Yi
HY
Li
Gang
G
Yu
Feng-Qin
FQ
D000818:Animals; D001812:Blood-Brain Barrier / Q000187:drug effects* / Q000378:metabolism; D006710:Homocysteine / Q000097:blood*; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D014815:Vitamins / Q000494:pharmacology*
D014815:Vitamins; D006710:Homocysteine; D020778:Matrix Metalloproteinase 2; C522363:Mmp2 protein, rat