Ultrasound-assisted extraction of phillyrin from Forsythia suspensa.
Forsythia suspensa (Thunb.) Vahl is one of the most widely used traditional Chinese medicines, and possesses important biological activities, such as antibacterial, antiviral, anti-inflammatory and antioxidant activities. Phillyrin is the main bioactive component of Forsythia suspensa. In this paper, ultrasound-assisted extraction of phillyrin from Forsythia suspensa was studied with HPLC-photodiode array detection. Effects of several experimental parameters, such as type and concentration of extracting solvent, ratio of liquid to material, extraction temperature, and time of sonication on extraction efficiencies of phillyrin from Forsythia suspensa were evaluated. The optimal extraction conditions were 1g plant sample with 10 ml of 20% methanol and the extraction for 60 min at 60°C under ultrasonic irradiation. Under the optimum conditions, the yield of phillyrin was 0.713±0.009 mg/g. The results indicated that the ultrasound-assisted extraction is a very useful method for the extraction of important phytochemicals from plant materials.
Xia
En-Qin
EQ
Guangdong Provincial Key Laboratory of Food, Nutrition and Health, School of Public Health, Sun Yat-Sen University, Guangzhou, China.
Ai
Xu-Xia
XX
Zang
Sheng-Ye
SY
Guan
Ting-Ting
TT
Xu
Xiang-Rong
XR
Li
Hua-Bin
HB
D015415:Biomarkers / Q000032:analysis; D005591:Chemical Fractionation / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D031657:Forsythia / Q000737:chemistry*; D005960:Glucosides / Q000032:analysis / Q000302:isolation & purification*; D012997:Solvents / Q000737:chemistry; D013010:Sonication; D013696:Temperature; D013997:Time Factors
D015415:Biomarkers; D004365:Drugs, Chinese Herbal; D005960:Glucosides; D012997:Solvents; C075528:phillyrin