title: Gum Guar fiber associated with fructose reduces serum triacylglycerol but did not improve the glucose tolerance in rats.
abstract: BACKGROUND
  The increased intake of dietary fructose can be associated with alterations on energy homeostasis and lipid/carbohydrate metabolism, such as insulin resistance and dislipidemia. On the other hand, the ingestion of soluble fiber gum guar could improve benefic mechanism on glucose tolerance and lipids profile.
  
  
  OBJECTIVE
  The aim of the present study were to investigate the effects of the supplemental feeding partially hydrolyzed gum guar on glucose and lipid homeostasis, in rats fed with fructose solution.
  
  
  METHODS
  The study was performed on thirty day-old male Wistar rats randomly assigned into four groups: control(C) or treated with fructose (F-20%), fiber (FB-5%), or fructose plus fiber (F-20% + FB-5% = FF) solution for 30 days on glucose tolerance (OGTT), triacylglycerol concentration in the liver by chloroform/methanol method, glucose, triacylglycerol and total cholesterol serum concentration by assayed by enzymatic colorimetric method, insulin receptor (IR) concentration in the liver by Western Blotting.
  
  
  RESULTS
  The total body weight gain was not different between groups; in regards of total caloric intake, in the F group was significantly higher and in the FB group was lower than other groups. The triacylglycerol concentration in the liver of FF group was significantly higher than F group, the triacylglycerol concentration in the serum was higher the F group compared with other groups. The OGTT reveal impaired on glucose tolerance in the F, FB, FF compared with C. The IR concentration in the liver was lower in the F, FB, FF compared with C, no significant difference was observed between groups for IR concentration in the gastrocnemius muscle. No significant difference was observed between groups for carcass fat content and serum total cholesterol.
  
  
  CONCLUSIONS
  Fructose induced important alterations on glucose tolerance and lipid metabolism, despite of fiber showed reversion of part this alterations. The association fructose plus fiber to seem decrease insulin receptor concentration in the liver, with consequent impair on glucose tolerance.
authors.affiliation: Departamento de Fisiologia, Universidade Federal de São Paulo - UNIFESP - Rua Botucatu, 862, 2nd floor, Edifício de Ciências Biomédicas, Vila Clementino, São Paulo, SP, Brazil. lira@unifesp.br.
authors.forename: Caio Sm
authors.initials: CS
authors.lastname: Motoyama
authors.forename: Mônica Js
authors.initials: MJ
authors.lastname: Pinto
authors.forename: Fabio S
authors.initials: FS
authors.lastname: Lira
authors.forename: Eliane B
authors.initials: EB
authors.lastname: Ribeiro
authors.forename: Claudia Mo
authors.initials: CM
authors.lastname: do Nascimento
authors.forename: Lila M
authors.initials: LM
authors.lastname: Oyama