title: Endogenous antioxidants and nasal human epithelium response to air pollutants: genotoxic and inmmuno-cytochemical evaluation.
abstract: Nasal epithelium is a source for identifying atmospheric pollution impact. Antioxidants play a relevant role in the protection of the cells from environmental injury, but scarce information is available about the interaction of endogenous antioxidants and genotoxic damage in nasal epithelium from urban populations highly exposed to traffic-generated air pollutants. An immunocytochemical and genotoxic evaluation was implemented in nasal cell epithelium in a population chronically exposed to atmospheric pollution from autumn 2004 to autumn 2005. Superoxide dismutase (SOD) and Catalase (CAT) were evaluated in nasal scrapings by morphometry and genotoxicity by comet assay. An increase in DNA damage correlates with a decrease in SOD and CAT in nasal cells during autumn and the inverse result was observed during summer (R = 0.88). Not only should exogenous antioxidant supplements be encouraged, but also a healthy diet to strengthen intracellular defenses against oxidative stress induced by exposure to air pollutants.
authors.affiliation: Cellular and Tissular Biology Department, School of Medicine, National University of Mexico, Mexico City, Mexico. fortoul@servidor.unam.mx
authors.forename: T I
authors.initials: TI
authors.lastname: Fortoul
authors.forename: M
authors.initials: M
authors.lastname: Rojas-Lemus
authors.forename: M C
authors.initials: MC
authors.lastname: Avila-Casado
authors.forename: V
authors.initials: V
authors.lastname: Rodriguez-Lara
authors.forename: L F
authors.initials: LF
authors.lastname: Montaño
authors.forename: A
authors.initials: A
authors.lastname: Muñoz-Comonfort
authors.forename: L S
authors.initials: LS
authors.lastname: Lopez-Zepeda
mesh_terms: D000393:Air Pollutants / Q000633:toxicity*; D000975:Antioxidants / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism*; D020552:Comet Assay; D004249:DNA Damage; D004787:Environmental Pollution; D005260:Female; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D009297:Nasal Mucosa / Q000187:drug effects*; D018384:Oxidative Stress / Q000502:physiology; D011446:Prospective Studies; D012621:Seasons; D013482:Superoxide Dismutase / Q000378:metabolism*; D014504:Urban Health; D055815:Young Adult
chemical_list: D000393:Air Pollutants; D000975:Antioxidants; D002374:Catalase; D013482:Superoxide Dismutase