[Effect of cardiopulmonary bypass on the expression of survivin in neutrophils].
OBJECTIVE
To investigate the effects of cardiopulmonary bypass on neutrophils apoptosis and the expression of survivin.


METHODS
Ten patients who scheduled for cardiac surgery under cardiopulmonary bypass were recruited as study group and 10 healthy volunteers as control. Blood samples were obtained before operation, at the end of surgery, and at 24 hours postoperatively. Neutrophils were isolated by density gradient centrifugation and its apoptosis were evaluated by fluorescent microscope and flow cytometry. The expression of survivin protein was examined by Western blotting analysis. Expression level of survivin mRNA was detected by RT-PCR.


RESULTS
The apoptotic rate of neutrophils decreased significantly at the end of surgery (P < 0.01), and was still lower at 24 hours postoperatively than before operation (P < 0.05). The expression ratios of survivin protein and mRNA were increased at the end of surgery (P < 0.01), and decreased gently at 24 hours postoperatively but was still higher than before operation (P < 0.05).


CONCLUSIONS
Cardiopulmonary bypass could inhibit neutrophils apoptosis and increase the expression of survivin. The decrease of neutrophils apoptosis was correlated with high expression of survivin.
Lin
Xue-Zheng
XZ
Department of Anesthesiology, Taizhou Central Hospital, Taizhou 318000, China. linxuezheng@163.com
Gong
Ming
M
Peng
Cong-Bin
CB
Zhou
Chun-Lian
CL
Cong
Hai-Tao
HT
Wang
Yu
Y
Song
Zhen-Dong
ZD
Lu
Guang-Tao
GT
D000328:Adult; D017209:Apoptosis; D002315:Cardiopulmonary Bypass; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins; D008297:Male; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D008875:Middle Aged; D009504:Neutrophils / Q000378:metabolism*; D000077022:Survivin
C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins; D000077022:Survivin