title: Ethylene stimulation of latex yield depends on the expression of a sucrose transporter (HbSUT1B) in rubber tree (Hevea brasiliensis).
abstract: Hevea brasiliensis is an important industrial crop for natural rubber production. Latex biosynthesis occurs in the cytoplasm of highly specialized latex cells and requires sucrose as the unique precursor. Ethylene stimulation of latex production results in high sugar flow from the surrounding cells of inner bark towards the latex cells. The aim of this work was to understand the role of seven sucrose transporters (HbSUTs) and one hexose transporter (HbHXT1) in this process. Two Hevea clones were used: PB217 and PB260, respectively described as high and low yielding clones. The expression pattern of these sugar transporters (HbSUTs and HbHXT1) was monitored under different physiological conditions and found to be maximal in latex cells. HbSUT1, one of the most abundant isoforms, displayed the greatest response to ethylene treatment. In clone PB217, ethylene treatment led to a higher accumulation of HbSUT1B in latex cells than in the inner bark tissues. Conversely, stronger expression of HbSUT1B was observed in inner bark tissues than in latex cells of PB260. A positive correlation with HbSUT1B transcript accumulation and increased latex production was further supported by its lower expression in latex cells of the virgin clone PB217.
authors.affiliation: Clermont Université, Université Blaise Pascal, UMRA 547 PIAF, F-63177 Aubière, France.
authors.forename: Anaïs
authors.initials: A
authors.lastname: Dusotoit-Coucaud
authors.forename: Panida
authors.initials: P
authors.lastname: Kongsawadworakul
authors.forename: Laurence
authors.initials: L
authors.lastname: Maurousset
authors.forename: Unshira
authors.initials: U
authors.lastname: Viboonjun
authors.forename: Nicole
authors.initials: N
authors.lastname: Brunel
authors.forename: Valérie
authors.initials: V
authors.lastname: Pujade-Renaud
authors.forename: Hervé
authors.initials: H
authors.lastname: Chrestin
authors.forename: Soulaïman
authors.initials: S
authors.lastname: Sakr
mesh_terms: D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D018744:DNA, Plant / Q000235:genetics; D005030:Ethylenes / Q000494:pharmacology*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D028482:Hevea / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D007840:Latex / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D009943:Organophosphorus Compounds / Q000494:pharmacology*; D010802:Phylogeny; D024301:Plant Bark; D010937:Plant Growth Regulators / Q000494:pharmacology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D018547:Plant Stems; D021381:Protein Transport; D013997:Time Factors
chemical_list: D018076:DNA, Complementary; D018744:DNA, Plant; D005030:Ethylenes; D007840:Latex; D026901:Membrane Transport Proteins; D009943:Organophosphorus Compounds; D010937:Plant Growth Regulators; D010940:Plant Proteins; C078729:sucrose transport protein, plant; C036216:ethylene; C005073:ethephon