Immune responses to adeno-associated virus type 2 encoding channelrhodopsin-2 in a genetically blind rat model for gene therapy.
We had previously reported that transduction of the channelrhodopsin-2 (ChR2) gene into retinal ganglion cells restores visual function in genetically blind, dystrophic Royal College of Surgeons (RCS) rats. In this study, we attempted to reveal the safety and influence of exogenous ChR2 gene expression. Adeno-associated virus (AAV) type 2 encoding ChR2 fused to Venus (rAAV-ChR2V) was administered by intra-vitreous injection to dystrophic RCS rats. However, rAAV-ChR2 gene expression was detected in non-target organs (intestine, lung and heart) in some cases. ChR2 function, monitored by recording visually evoked potentials, was stable across the observation period (64 weeks). No change in retinal histology and no inflammatory marker of leucocyte adhesion in the retinal vasculature were observed. Although antibodies to rAAV (0.01-12.21 μg ml(-1)) and ChR2 (0-4.77 μg ml(-1)) were detected, their levels were too low for rejection. T-lymphocyte analysis revealed recognition by T cells and a transient inflammation-like immune reaction only until 1 month after the rAAV-ChR2V injection. In conclusion, ChR2, which originates from Chlamydomonas reinhardtii, can be expressed without immunologically harmful reactions in vivo. These findings will help studies of ChR2 gene transfer to restore vision in progressed retinitis pigmentosa.
Sugano
E
E
Department of International Advanced Interdisciplinary Research, Institute for International Advanced Research and Education, Tohoku University, Sendai, Japan.
Isago
H
H
Wang
Z
Z
Murayama
N
N
Tamai
M
M
Tomita
H
H
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D017931:DNA Primers / Q000235:genetics; D000229:Dependovirus / Q000235:genetics / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005074:Evoked Potentials, Visual / Q000502:physiology*; D015316:Genetic Therapy / Q000379:methods*; D007150:Immunohistochemistry; D008856:Microscopy, Fluorescence; D051381:Rats; D012174:Retinitis Pigmentosa / Q000235:genetics / Q000628:therapy*; D012243:Rhodopsin / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology; D014161:Transduction, Genetic
D000914:Antibodies, Viral; D017931:DNA Primers; D012243:Rhodopsin