Labdane-type diterpenoids from Leonurus heterophyllus and their cholinesterase inhibitory activity.
In the course of screening plants used in natural medicines as memory enhancers, a 70% ethanol extract of the aerial parts of Leonurus heterophyllus showed significant AChE inhibitory activity. Bioassay-guided fractionation and repeated column chromatography led to the isolation of a new labdane-type diterpenoids (1), named leoheteronin F, and six known compounds (2-7). The chemical structures of isolated compounds were elucidated based on extensive 1D and 2D NMR spectroscopic data. The isolates 1-7 were investigated in vitro for their anticholinesterase activity using mouse cortex AChE enzyme. Leoheteronin A (5) and leopersin G (7), which possess a 15,16-epoxy group at the side chain, were found to be potent in the inhibition of AChE.
Hung
Tran Manh
TM
College of Pharmacy, Catholic University of Daegu, Gyeongbuk 712-702, Korea. tmhung1810@yahoo.com
Luan
Tran Cong
TC
Vinh
Bui The
BT
Cuong
To Dao
TD
Min
Byung Sun
BS
D000818:Animals; D002800:Cholinesterase Inhibitors / Q000494:pharmacology*; D004224:Diterpenes / Q000494:pharmacology*; D031332:Leonurus / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
D002800:Cholinesterase Inhibitors; D004224:Diterpenes