Reflections on the development of micronucleus assays.
These are personal reflections on the development of methods to use micronuclei as a measure of genetic damage and their use in research and in toxicology by four people who have been intimately involved with this work, a personal rather than a comprehensive history. About 6000 papers have been published using such methods in many tissues in vivo or in cultured cells of many organisms from plants to humans, but the majority of the work has been on mammalian erythrocytes and human lymphocytes, the areas in which we have worked primarily. Although this is by no means a complete history, those working in the field may be interested in some of the personal events that lie behind the development and acceptance of methods that are now standard.
Heddle
John A
JA
Department of Biology, York University, Toronto, Ontario M3J 1P3, Canada.
Fenech
Michael
M
Hayashi
Makoto
M
MacGregor
James T
JT
D000818:Animals; D004249:DNA Damage; D049673:History, 20th Century; D006801:Humans; D051379:Mice; D048629:Micronuclei, Chromosome-Defective; D015162:Micronucleus Tests / Q000266:history*