Identification by genotyping of a commercial antigen preparation as the source of a laboratory contamination with Coxiella burnetii and as an unexpected rich source of control DNA.
By performing genotyping, a laboratory contamination involving Q fever was traced back to the antigen preparation used in a commercially available complement fixation test. It was established that such antigen preparations contain relatively high loads of DNA/RNA, making them potential sources of contamination but also convenient preparations for control material.
Tilburg
Jeroen J H C
JJ
Department of Medical Microbiology and Infectious Diseases, Canisius Wilhelmina Hospital, Weg door Jonkerbos 100, 6532 SZ Nijmegen, Netherlands.
Horrevorts
Alphons M
AM
Peeters
Marcel F
MF
Klaassen
Corné H W
CH
Rossen
John W A
JW
D003168:Complement Fixation Tests; D016997:Coxiella burnetii / Q000145:classification* / Q000235:genetics*; D059018:DNA Contamination; D006801:Humans; D011933:Reagent Kits, Diagnostic / Q000382:microbiology*
D011933:Reagent Kits, Diagnostic