title: Human B cell development and antibody production in humanized NOD/SCID/IL-2Rγ(null) (NSG) mice conditioned by busulfan.
abstract: BACKGROUND
  Busulfan treatment as a chemotherapeutic agent has been considered an alternative approach in xenograft model because it offers a simple, convenient, effective, and less toxic conditioning regimen.
  
  
  OBJECTIVE
  To investigate busulfan effects on the reconstitution of human immune cells and the generation of immune response to foreign antigens, we generated humanized NOD/SCID/IL-2Rγ(null) (NSG) mice conditioned either busulfan or total body irradiation (TBI) with hCD34(+) CB cells.
  
  
  RESULTS
  Busulfan resulted in a high survival rate and effective reconstitution of human immune cells including B, T, macrophage, and dendritic cells in humanized NSG mice, compared to that of TBI. Moreover, the humanized NSG mice conditioned busulfan showed effective B cell development and thereby the high production of human antibody against immunized antigen.
  
  
  CONCLUSIONS
  Humanized mice conditioned by busulfan provide a powerful and versatile tool for studying the entire process of human B-lymphocyte development and for producing specific human antibodies.
authors.affiliation: Department of Molecular Medicine, Sungkyunkwan University School of Medicine, Seoul 135-710, Republic of Korea.
authors.forename: Bongkum
authors.initials: B
authors.lastname: Choi
authors.forename: Eunyoung
authors.initials: E
authors.lastname: Chun
authors.forename: Miyoung
authors.initials: M
authors.lastname: Kim
authors.forename: Seong-Tae
authors.initials: ST
authors.lastname: Kim
authors.forename: Keejung
authors.initials: K
authors.lastname: Yoon
authors.forename: Ki-Young
authors.initials: KY
authors.lastname: Lee
authors.forename: Sung Joo
authors.initials: SJ
authors.lastname: Kim
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000818:Animals; D000917:Antibody Formation / Q000187:drug effects* / Q000276:immunology* / Q000528:radiation effects; D001402:B-Lymphocytes / Q000166:cytology* / Q000187:drug effects / Q000276:immunology* / Q000528:radiation effects; D002066:Busulfan / Q000494:pharmacology*; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D016867:Immunocompromised Host / Q000187:drug effects* / Q000528:radiation effects; D053631:Interleukin Receptor Common gamma Subunit / Q000235:genetics; D007928:Lethal Dose 50; D008198:Lymph Nodes / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D051379:Mice; D016688:Mice, Inbred NOD; D018345:Mice, Knockout; D016513:Mice, SCID; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D016019:Survival Analysis; D013601:T-Lymphocytes / Q000276:immunology; D014916:Whole-Body Irradiation
chemical_list: D000276:Adjuvants, Immunologic; D053631:Interleukin Receptor Common gamma Subunit; D002066:Busulfan