[Study of HBV-DNA in ovarian granulosa cells of hepatitis B carriers].
OBJECTIVE
To study the infection status of hepatitis B virus in ovarian granulosa cells of female hepatitis B carriers.


METHODS
The follicular fluid of 6 female carriers of hepatitis B (HBsAg+, HBeAg+, HBcAb+) was used to extract granule cells and prepare smears. The samples were detected via in situ hybridization by digoxin-labeled HBV-DNA probe. The color reaction system of alkaline phosphates was used. And the positive hybridization signal was purple blue.


RESULTS
Three granulosa cell samples, located at the nuclei of granulosa cells, were HBV-DNA positive.


CONCLUSIONS
HBV DNA exists in the granulosa cells of hepatitis B patients. Thus HBV infection may be vertically transmitted to the offspring via the infected reproductive cells.
Mei
Shun-li
SL
Reproductive Research Center, Peking University Third Hospital, Beijing 100191, China.
Huang
Jin
J
Liu
Ping
P
Zhang
Xiao-wei
XW
Qiao
Jie
J
Zhang
Qiu-fang
QF
Zhang
Ying
Y
D000328:Adult; D002353:Carrier State / Q000821:virology*; D054885:Cumulus Cells / Q000821:virology*; D015342:DNA Probes; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006509:Hepatitis B / Q000821:virology*; D006515:Hepatitis B virus / Q000235:genetics; D006801:Humans; D017403:In Situ Hybridization
D015342:DNA Probes; D004279:DNA, Viral