title: Variation of GmIRCHS (Glycine max inverted-repeat CHS pseudogene) is related to tolerance of low temperature-induced seed coat discoloration in yellow soybean.
abstract: In yellow soybean, seed coat pigmentation is inhibited by post-transcriptional gene silencing (PTGS) of chalcone synthase (CHS) genes. A CHS cluster named GmIRCHS (Glycine max inverted-repeat CHS pseudogene) is suggested to cause PTGS in yellow-hilum cultivars. Cold-induced seed coat discoloration (CD), a commercially serious deterioration of seed appearance, is caused by an inhibition of this PTGS upon exposure to low temperatures. In the highly CD-tolerant cultivar Toyoharuka, the GmIRCHS structure differs from that of other cultivars. The aim of this study was to determine whether the variation of GmIRCHS structure among cultivars is related to variations in CD tolerance. Using two sets of recombinant inbred lines between Toyoharuka and CD-susceptible cultivars, we compared the GmIRCHS genotype and CD tolerance phenotype during low temperature treatment. The GmIRCHS genotype was related to the phenotype of CD tolerance. A QTL analysis around GmIRCHS showed that GmIRCHS itself or a region located very close to it was responsible for CD tolerance. The variation in GmIRCHS can serve as a useful DNA marker for marker-assisted selection for breeding CD tolerance. In addition, QTL analysis of the whole genome revealed a minor QTL that also affected CD tolerance.
authors.affiliation: Hokkaido Research Organization Tokachi Agricultural Experiment Station, Shinsei, Memuro, Kasai-gun, Hokkaido, Japan. ohnishi-shizen@hro.or.jp
authors.forename: Shizen
authors.initials: S
authors.lastname: Ohnishi
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Funatsuki
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kasai
authors.forename: Tasuku
authors.initials: T
authors.lastname: Kurauchi
authors.forename: Naoya
authors.initials: N
authors.lastname: Yamaguchi
authors.forename: Toru
authors.initials: T
authors.lastname: Takeuchi
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Yamazaki
authors.forename: Hideki
authors.initials: H
authors.lastname: Kurosaki
authors.forename: Shigehisa
authors.initials: S
authors.lastname: Shirai
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Miyoshi
authors.forename: Harukuni
authors.initials: H
authors.lastname: Horita
authors.forename: Mineo
authors.initials: M
authors.lastname: Senda
mesh_terms: D000217:Acyltransferases / Q000235:genetics*; D000222:Adaptation, Physiological / Q000235:genetics*; D003080:Cold Temperature; D005819:Genetic Markers; D014644:Genetic Variation; D005838:Genotype; D007178:Inbreeding; D055029:Inverted Repeat Sequences / Q000235:genetics*; D010641:Phenotype; D010858:Pigmentation / Q000235:genetics*; D011544:Pseudogenes / Q000235:genetics; D040641:Quantitative Trait Loci / Q000235:genetics; D012044:Regression Analysis; D012639:Seeds / Q000235:genetics*; D013025:Glycine max / Q000201:enzymology / Q000235:genetics*
chemical_list: D005819:Genetic Markers; D000217:Acyltransferases; C020141:flavanone synthetase