title: Cryo-EM structure and rRNA model of a translating eukaryotic 80S ribosome at 5.5-A resolution.
abstract: Protein biosynthesis, the translation of the genetic code into polypeptides, occurs on ribonucleoprotein particles called ribosomes. Although X-ray structures of bacterial ribosomes are available, high-resolution structures of eukaryotic 80S ribosomes are lacking. Using cryoelectron microscopy and single-particle reconstruction, we have determined the structure of a translating plant (Triticum aestivum) 80S ribosome at 5.5-Å resolution. This map, together with a 6.1-Å map of a Saccharomyces cerevisiae 80S ribosome, has enabled us to model ∼98% of the rRNA. Accurate assignment of the rRNA expansion segments (ES) and variable regions has revealed unique ES-ES and r-protein-ES interactions, providing insight into the structure and evolution of the eukaryotic ribosome.
authors.affiliation: Gene Center, Department of Biochemistry, Ludwig-Maximilians-Universität München, Feodor-Lynen-Strasse 25, 81377 Munich, Germany.
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Armache
authors.forename: Alexander
authors.initials: A
authors.lastname: Jarasch
authors.forename: Andreas M
authors.initials: AM
authors.lastname: Anger
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Villa
authors.forename: Thomas
authors.initials: T
authors.lastname: Becker
authors.forename: Shashi
authors.initials: S
authors.lastname: Bhushan
authors.forename: Fabrice
authors.initials: F
authors.lastname: Jossinet
authors.forename: Michael
authors.initials: M
authors.lastname: Habeck
authors.forename: Gülcin
authors.initials: G
authors.lastname: Dindar
authors.forename: Sibylle
authors.initials: S
authors.lastname: Franckenberg
authors.forename: Viter
authors.initials: V
authors.lastname: Marquez
authors.forename: Thorsten
authors.initials: T
authors.lastname: Mielke
authors.forename: Michael
authors.initials: M
authors.lastname: Thomm
authors.forename: Otto
authors.initials: O
authors.lastname: Berninghausen
authors.forename: Birgitta
authors.initials: B
authors.lastname: Beatrix
authors.forename: Johannes
authors.initials: J
authors.lastname: Söding
authors.forename: Eric
authors.initials: E
authors.lastname: Westhof
authors.forename: Daniel N
authors.initials: DN
authors.lastname: Wilson
authors.forename: Roland
authors.initials: R
authors.lastname: Beckmann
mesh_terms: D020285:Cryoelectron Microscopy; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000378:metabolism / Q000648:ultrastructure; D005057:Eukaryotic Cells / Q000378:metabolism / Q000648:ultrastructure*; D006801:Humans; D008958:Models, Molecular; D014176:Protein Biosynthesis; D012335:RNA, Ribosomal / Q000648:ultrastructure*; D012270:Ribosomes / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure*; D012441:Saccharomyces cerevisiae / Q000378:metabolism / Q000648:ultrastructure; D014908:Triticum / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D012335:RNA, Ribosomal