title: Identification by genotyping of a commercial antigen preparation as the source of a laboratory contamination with Coxiella burnetii and as an unexpected rich source of control DNA.
abstract: By performing genotyping, a laboratory contamination involving Q fever was traced back to the antigen preparation used in a commercially available complement fixation test. It was established that such antigen preparations contain relatively high loads of DNA/RNA, making them potential sources of contamination but also convenient preparations for control material.
authors.affiliation: Department of Medical Microbiology and Infectious Diseases, Canisius Wilhelmina Hospital, Weg door Jonkerbos 100, 6532 SZ Nijmegen, Netherlands.
authors.forename: Jeroen J H C
authors.initials: JJ
authors.lastname: Tilburg
authors.forename: Alphons M
authors.initials: AM
authors.lastname: Horrevorts
authors.forename: Marcel F
authors.initials: MF
authors.lastname: Peeters
authors.forename: Corné H W
authors.initials: CH
authors.lastname: Klaassen
authors.forename: John W A
authors.initials: JW
authors.lastname: Rossen
mesh_terms: D003168:Complement Fixation Tests; D016997:Coxiella burnetii / Q000145:classification* / Q000235:genetics*; D059018:DNA Contamination; D006801:Humans; D011933:Reagent Kits, Diagnostic / Q000382:microbiology*
chemical_list: D011933:Reagent Kits, Diagnostic