title: Evaluating cell proliferation based on internal pore size and 3D scaffold architecture fabricated using solid freeform fabrication technology.
abstract: The scaffold, as a medical component to regenerate tissues or organs in humans, plays an important role in tissue engineering. Recently, solid freeform fabrication (SFF) technology using computer-assisted methods was applied to address the problems of conventional fabrication methods in which the internal/outer architectures cannot be controlled. In this report, we propose suitable scaffolds for bone tissue regeneration considering the internal pore size and scaffold architecture. Poly(propylene fumarate) was used as the biodegradable photopolymer, and scaffolds were fabricated using microstereolithography (MSTL). We observed the relationship between the internal pores and architecture, and the proliferation of pre-osteoblast cells. To demonstrate the superiority of MSTL, we fabricated conventional and SFF scaffolds, and measured the cell proliferation rates for each. The results showed that cell proliferation on the MSTL scaffold was clearly superior and indicated that MSTL would be a good replacement for current conventional methods.
authors.affiliation: Department of Mechanical Engineering, The University of Texas at Austin, Austin, TX 78712-0292, USA.
authors.forename: Jin Woo
authors.initials: JW
authors.lastname: Lee
authors.forename: Geunseon
authors.initials: G
authors.lastname: Ahn
authors.forename: Jong Young
authors.initials: JY
authors.lastname: Kim
authors.forename: Dong-Woo
authors.initials: DW
authors.lastname: Cho
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D002478:Cells, Cultured; D006801:Humans; D008420:Manufactured Materials / Q000032:analysis; D008422:Materials Testing / Q000379:methods*; D051379:Mice; D055616:Microtechnology / Q000379:methods*; D008954:Models, Biological; D010316:Particle Size; D016062:Porosity; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*