Cryo-EM structure and rRNA model of a translating eukaryotic 80S ribosome at 5.5-A resolution.
Protein biosynthesis, the translation of the genetic code into polypeptides, occurs on ribonucleoprotein particles called ribosomes. Although X-ray structures of bacterial ribosomes are available, high-resolution structures of eukaryotic 80S ribosomes are lacking. Using cryoelectron microscopy and single-particle reconstruction, we have determined the structure of a translating plant (Triticum aestivum) 80S ribosome at 5.5-Å resolution. This map, together with a 6.1-Å map of a Saccharomyces cerevisiae 80S ribosome, has enabled us to model ∼98% of the rRNA. Accurate assignment of the rRNA expansion segments (ES) and variable regions has revealed unique ES-ES and r-protein-ES interactions, providing insight into the structure and evolution of the eukaryotic ribosome.
Armache
Jean-Paul
JP
Gene Center, Department of Biochemistry, Ludwig-Maximilians-Universität München, Feodor-Lynen-Strasse 25, 81377 Munich, Germany.
Jarasch
Alexander
A
Anger
Andreas M
AM
Villa
Elizabeth
E
Becker
Thomas
T
Bhushan
Shashi
S
Jossinet
Fabrice
F
Habeck
Michael
M
Dindar
Gülcin
G
Franckenberg
Sibylle
S
Marquez
Viter
V
Mielke
Thorsten
T
Thomm
Michael
M
Berninghausen
Otto
O
Beatrix
Birgitta
B
Söding
Johannes
J
Westhof
Eric
E
Wilson
Daniel N
DN
Beckmann
Roland
R
D020285:Cryoelectron Microscopy; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000378:metabolism / Q000648:ultrastructure; D005057:Eukaryotic Cells / Q000378:metabolism / Q000648:ultrastructure*; D006801:Humans; D008958:Models, Molecular; D014176:Protein Biosynthesis; D012335:RNA, Ribosomal / Q000648:ultrastructure*; D012270:Ribosomes / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure*; D012441:Saccharomyces cerevisiae / Q000378:metabolism / Q000648:ultrastructure; D014908:Triticum / Q000378:metabolism / Q000648:ultrastructure
D012335:RNA, Ribosomal