Quantitative detection of enterotoxigenic Bacteroides fragilis subtypes isolated from children with and without diarrhea.
A rapid real-time PCR (RT-PCR) approach was developed to detect the bft gene subtypes in Bacteroides fragilis isolated from fecal samples. DNA obtained from diarrhea (110) and nondiarrhea (150) samples was evaluated. Subtype 1 was observed in 9 (8.2%) diarrhea and 7 (4.7%) nondiarrhea samples. Subtype 2 was not detected in any DNA samples, and subtype 3 was observed in only 1 diarrhea sample. The presence of the bft-1 gene did not show any statistically significant differences between the groups of children. This technique could be used to evaluate a possible correlation between disease and the presence of B. fragilis enterotoxin.
Merino
V R C
VR
Anaerobe Laboratory, Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, Av. Prof. Lineu Prestes 1374, 05508-900 São Paulo, SP, Brazil.
Nakano
V
V
Liu
C
C
Song
Y
Y
Finegold
S M
SM
Avila-Campos
M J
MJ
D001427:Bacterial Toxins / Q000235:genetics; D001442:Bacteroides Infections / Q000382:microbiology*; D001441:Bacteroides fragilis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D002648:Child; D002675:Child, Preschool; D003967:Diarrhea / Q000382:microbiology*; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D008666:Metalloendopeptidases / Q000235:genetics; D058889:Molecular Typing / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods
D001427:Bacterial Toxins; C405031:Bacteroides fragilis toxin; D008666:Metalloendopeptidases