[Study on the activity against influenza A virus with traditional Chinese medicine Kanggan granules].
OBJECTIVE
To study the anti-influenza A virus effects of traditional Chinese medicine Kanggan granules in chicken embryo and BALB/c mice.


METHODS
The influenza A virus (H(1)N(1), FM1) was used in the experiments. FM1 was cultured in chicken embryo and the anti-FM1 activity of Kanggan granules was evaluated through the post-medication hemagglutination titer of FM1. In animal test, 120 healthy BALB/c mice were randomly divided into six groups, normal control, virus control, ribavirin, high-dosage, middle-dosage and low-dosage. The FM1 infection model was established by dripping FM1 into nasal cavity and then the appropriate treatments were prescribed. The effective anti- FM1 indices of Kanggan granules included survival status, protective percentage of death and life elongation percentage of mice infected with FM1.


RESULTS
The high and middle doses of Kanggan granules could inhibit the replication of FM1 remarkably in chicken embryo, and could reduced hemagglutination titer to 5 and 3 times. In animal experiments, all mice treated with Kanggan granules could improve the general status of infected mice, the protective percentages of death were 35.0% to 55.0%, the life elongation percentages were 73.0% to 88.9% and the minimal effective dose was 3.00 g/kg.


CONCLUSIONS
Kanggan granules can inhibit the replication of influenza A virus and protect the mice infected with FM1.
Liu
Feng-huan
FH
Department of Microbiology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Li
Ming-yuan
MY
Luo
Jun
J
Li
Wan-yi
WY
Wamg
Bao-ning
BN
Yang
Yuan
Y
Kuang
Yu
Y
Zuo
Bin
B
Ma
Xiu-ying
XY
Jiang
Zhong-hua
ZH
D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology* / Q000633:toxicity; D002642:Chick Embryo; D004365:Drugs, Chinese Herbal / Q000494:pharmacology* / Q000633:toxicity; D053118:Influenza A Virus, H1N1 Subtype / Q000187:drug effects*; D007928:Lethal Dose 50; D051379:Mice; D008807:Mice, Inbred BALB C
D000998:Antiviral Agents; D004365:Drugs, Chinese Herbal