Cycloheximide-mediated superinduction of genes involves both native and foreign transcripts in rice (Oryza sativa L.).
Rice seedlings subjected to heat shock show rapid and transient induction of Oshsp17.4-CI, Oshsp17.9A-CI and OsClpB-cyt/hsp100 transcripts. When the seedlings were pre-treated with protein synthesis inhibitor cycloheximide, levels of the above transcripts during heat shock were more elevated than those seen with heat shock alone. Heat stress and cycloheximide co-treatment resulted in higher transcript accumulation in comparison to cycloheximide pre-treatment followed by heat stress. In transgenic plants raised with OsClpB-cyt/hsp100 promoter driving expression of the reporter gus gene, expression of the gus transcript was subjected to similar superinduction event as was seen with native OsClpB-cyt/hsp100 transcripts in untransformed plants. Yeast cells transformed with variably-sized rice ClpB-cyt/hsp100 promoter driving expression of the lacZ reporter transcript showed that specific sequences of the promoter region may be implicated in superinduction.
Agarwal
Manu
M
Department of Plant Molecular Biology, University of Delhi South Campus, New Delhi 110021, India.
Singh
Amanjot
A
Mittal
Dheeraj
D
Sahi
Chandan
C
Grover
Anil
A
D000222:Adaptation, Physiological / Q000187:drug effects / Q000235:genetics*; D001483:Base Sequence; D003513:Cycloheximide / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D017343:Genes, Plant; D017930:Genes, Reporter; D006360:Heat-Shock Proteins / Q000235:genetics* / Q000378:metabolism; D007763:Lac Operon; D012275:Oryza / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D036226:Seedlings; D013312:Stress, Physiological / Q000235:genetics; D014170:Transformation, Genetic; D015003:Yeasts / Q000235:genetics / Q000378:metabolism
D006360:Heat-Shock Proteins; D010940:Plant Proteins; D011500:Protein Synthesis Inhibitors; D003513:Cycloheximide