Infrared microspectroscopy of live cells in aqueous media.
Fourier Transform Infrared (FTIR) spectroscopic measurements of individual, live HeLa cells in culture and buffer media are presented. Spectral data were acquired using a newly designed live cell chamber developed in the authors' laboratory. Data were processed using MATLAB-based routines that correct for the overcompensation of water encountered during live cell measurements in aqueous samples. Data presented are from live cells monitored over an extended period of time as well as a comparison of live cells exposed to perturbing conditions.
Marcsisin
Ellen J Swain
EJ
Laboratory for Spectral Diagnosis, Department of Chemistry and Chemical Biology, Northeastern University, Boston, MA 02115, USA.
Uttero
Christina M
CM
Miljković
Miloš
M
Diem
Max
M
D018929:Cell Culture Techniques; D003470:Culture Media / Q000737:chemistry*; D006367:HeLa Cells / Q000737:chemistry*; D006801:Humans; D017550:Spectroscopy, Fourier Transform Infrared / Q000295:instrumentation / Q000379:methods*; D014867:Water / Q000737:chemistry*
D003470:Culture Media; D014867:Water