Human B cell development and antibody production in humanized NOD/SCID/IL-2Rγ(null) (NSG) mice conditioned by busulfan.
BACKGROUND
Busulfan treatment as a chemotherapeutic agent has been considered an alternative approach in xenograft model because it offers a simple, convenient, effective, and less toxic conditioning regimen.


OBJECTIVE
To investigate busulfan effects on the reconstitution of human immune cells and the generation of immune response to foreign antigens, we generated humanized NOD/SCID/IL-2Rγ(null) (NSG) mice conditioned either busulfan or total body irradiation (TBI) with hCD34(+) CB cells.


RESULTS
Busulfan resulted in a high survival rate and effective reconstitution of human immune cells including B, T, macrophage, and dendritic cells in humanized NSG mice, compared to that of TBI. Moreover, the humanized NSG mice conditioned busulfan showed effective B cell development and thereby the high production of human antibody against immunized antigen.


CONCLUSIONS
Humanized mice conditioned by busulfan provide a powerful and versatile tool for studying the entire process of human B-lymphocyte development and for producing specific human antibodies.
Choi
Bongkum
B
Department of Molecular Medicine, Sungkyunkwan University School of Medicine, Seoul 135-710, Republic of Korea.
Chun
Eunyoung
E
Kim
Miyoung
M
Kim
Seong-Tae
ST
Yoon
Keejung
K
Lee
Ki-Young
KY
Kim
Sung Joo
SJ
D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000818:Animals; D000917:Antibody Formation / Q000187:drug effects* / Q000276:immunology* / Q000528:radiation effects; D001402:B-Lymphocytes / Q000166:cytology* / Q000187:drug effects / Q000276:immunology* / Q000528:radiation effects; D002066:Busulfan / Q000494:pharmacology*; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D016867:Immunocompromised Host / Q000187:drug effects* / Q000528:radiation effects; D053631:Interleukin Receptor Common gamma Subunit / Q000235:genetics; D007928:Lethal Dose 50; D008198:Lymph Nodes / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D051379:Mice; D016688:Mice, Inbred NOD; D018345:Mice, Knockout; D016513:Mice, SCID; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D016019:Survival Analysis; D013601:T-Lymphocytes / Q000276:immunology; D014916:Whole-Body Irradiation
D000276:Adjuvants, Immunologic; D053631:Interleukin Receptor Common gamma Subunit; D002066:Busulfan