Integration of normal phase liquid chromatography with supercritical fluid chromatography for analysis of fruiting bodies of Ganoderma lucidum.
In this study, a comprehensive 2-D chromatography was constructed, consisting of normal phase LC (NPLC) with a CN column as the first dimension, and supercritical fluid chromatography (SFC), with a Merck Chromolith Flash C(18) column as the second dimension, which were connected by a 10-port, dual-position valve controlled automatically by a self-designed software. Such platform was applied into the analysis of the fruiting bodies of Ganoderma lucidum, a traditional Chinese medicine, and within 2 h analysis, the obtained peak capacity of the 2-D-NPLC-SFC system was about 350, obviously higher than that of each dimension. These results demonstrate that 2-D-NPLC-SFC is not only of good orthogonality, but also of high throughput for the analysis of complex samples.
Gao
Liang
L
Key Laboratory of Separation Science for Analytical Chemistry, National Chromatographic Research and Analysis Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, PR China.
Zhang
Jie
J
Zhang
Weibing
W
Shan
Yichu
Y
Liang
Zhen
Z
Zhang
Lihua
L
Huo
Yushu
Y
Zhang
Yukui
Y
D002853:Chromatography, Liquid / Q000379:methods*; D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D020940:Reishi / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet