Molecular mass spectrometry imaging in biomedical and life science research.
This review describes the current state of mass spectrometry imaging (MSI) in life sciences. A brief overview of mass spectrometry principles is presented followed by a thorough introduction to the MSI workflows, principles and areas of application. Three major desorption-ionization techniques used in MSI, namely, secondary ion mass spectrometry (SIMS), matrix-assisted laser desorption ionization (MALDI), and desorption electrospray ionization (DESI) are described, and biomedical and life science imaging applications of each ionization technique are reviewed. A separate section is devoted to data handling and current challenges and future perspectives are briefly discussed at the end.
Pól
Jaroslav
J
Laboratory of Molecular Structure Characterization, Institute of Microbiology of the Academy of the ASCR, v.v.i., Vídeňská 1083, 142 20, Prague, Czech Republic.
Strohalm
Martin
M
Havlíček
Vladimír
V
Volný
Michael
M
D000818:Animals; D001690:Biological Science Disciplines / Q000379:methods*; D035843:Biomedical Research / Q000379:methods*; D006801:Humans; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation / Q000379:methods*; D018629:Spectrometry, Mass, Secondary Ion / Q000295:instrumentation / Q000379:methods*