Phylogenetic analysis and inflow route of Tomato yellow leaf curl virus (TYLCV) and Bemisia tabaci in Korea.
Tomato yellow leaf curl virus (TYLCV) is a member of the genus Begomovirus of the family Geminiviridae, members of which are characterized by closed circular single-stranded DNA genomes of 2.7-2.8 kb in length, and include viruses transmitted by the Bemisia tabaci whitefly. No reports of TYLCV in Korea are available prior to 2008, after which TYLCV spread rapidly to most regions of the southern Korean peninsula (Gyeongsang-Do, Jeolla-Do and Jeju-Do). Fifty full sequences of TYLCV were analyzed in this study, and the AC1, AV1, IR, and full sequences were analyzed via the muscle program and bayesian analysis. Phylogenetic analysis demonstrated that the Korea TYLCVs were divided into two subgroups. The TYLCV Korea 1 group (Masan) originated from TYLCV Japan (Miyazaki) and the TYLCV Korea 2 group (Jeju/Jeonju) from TYLCV Japan (Tosa/Haruno). A B. tabaci phylogenetic tree was constructed with 16S rRNA and mitochondria cytochrome oxidase I (MtCOI) sequences using the muscle program and MEGA 4.0 in the neighbor-joining algorithm. The sequence data of 16S rRNA revealed that Korea B. tabaci was closely aligned to B. tabaci isolated in Iran and Nigeria. The Q type of B. tabaci, which was originally identified as a viruliferous insect in 2008, was initially isolated in Korea as a non-viruliferous insect in 2005. Therefore, we suggest that two TYLCV Japan isolates were introduced to Korea via different routes, and then transmitted by native B. tabaci.
Lee
Hyejung
H
Department of Genetic Engineering, Sungkyunkwan University, Suwon, 440-746, Korea.
Song
Woogeun
W
Kwak
Hae-Ryun
HR
Kim
Jae-Deok
JD
Park
Jungan
J
Auh
Chung-Kyoon
CK
Kim
Dae-Hyun
DH
Lee
Kyeong-Yeoll
KY
Lee
Sukchan
S
Choi
Hong-Soo
HS
D000818:Animals; D001483:Base Sequence; D058893:DNA Barcoding, Taxonomic; D004277:DNA, Single-Stranded / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D003576:Electron Transport Complex IV / Q000235:genetics; D006430:Hemiptera / Q000821:virology*; D007303:Insect Vectors / Q000235:genetics; D018551:Solanum lycopersicum / Q000821:virology*; D008928:Mitochondria / Q000235:genetics; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000821:virology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D014764:Viral Proteins / Q000235:genetics*; D014777:Virus Diseases / Q000821:virology
D004277:DNA, Single-Stranded; D004279:DNA, Viral; D012336:RNA, Ribosomal, 16S; D014764:Viral Proteins; C080288:replication protein AL1, Begomovirus; D003576:Electron Transport Complex IV