[The effect of HCPT combined with radiation on cell proliferation of human lung adenocarcinoma cell line (D₆) in vitro].
BACKGROUND
To study the adjuvant effects of HCPT and radiation on cell proliferation of human lung adenocarcinoma cell line (D₆) in vitro.


METHODS
Suppression rate of cell growth,apoptosis and cell cycle of human pulmonary adenocarcinoma cell line were detected by MTT assay,DNA agrose electrophoresis and flow-cytometry respectively.


RESULTS
The suppression effects of HCPT on cell proliferation were dose and time dependent.The cells were significantly blocked at S stage by HCPT.Apoptosis peak appeared at 24 and 48 hours after treatment with high concentration (50μg/ml) and low concentration of HCPT (1μg/ml) respectively.DNA "Ladder" started to appear at 48 hours by radiation alone,but DNA "Ladder" and apoptosis peak both appeared at 24-72 hours and 0-72 hours after treatment with HCPT alone and HCPT combined with radiation respectively.


CONCLUSIONS
HCPT combined with radiation may show the synergistic killing effect on human lung adenocarcinoma cell line (D₆) in vitro.
Ma
S
S
Department of Radiation Oncology,Zhejiang Cancer Hospital,Hangzhou,Zhejiang 310022,P.R.China.
Dai
H
H
Chen
X
X
Feng
J
J