Polypyrimidine tract-binding protein 2 binds to selective, intronic messenger RNA and microRNA targets in the mouse testis.
Here we use an in vivo cross-linking and immunoprecipitation procedure to detect RNA targets of the multifunctional RNA-binding protein polypyrimidine tract-binding protein (PTBP) 2 in mouse testis. Eleven known mRNAs, including Ptbp2 mRNA, 28 RNAs matching intron sequences, and 12 small RNAs and repeat sequences are identified. The specificity of interaction between PTBP2 and its target RNAs was confirmed using RNA interference with mouse N2A cells. Reduction of PTBP2 levels led to decreases in 7 of 10 of the mRNAs, to the repression of alternative splicing of introns, and to reductions in specific miRNAs.
Xu
Mingang
M
Center for Research on Reproduction and Women's Health, University of Pennsylvania School of Medicine, 421 Curie Boulevard, Philadelphia, PA 19104-6080, USA.
Hecht
Norman B
NB
D017398:Alternative Splicing / Q000502:physiology; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003432:Cross-Linking Reagents / Q000494:pharmacology; D005786:Gene Expression Regulation; D047468:Immunoprecipitation / Q000379:methods; D047668:Inteins; D008297:Male; D051379:Mice; D035683:MicroRNAs / Q000378:metabolism*; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D038941:Polypyrimidine Tract-Binding Protein / Q000378:metabolism*; D011485:Protein Binding; D012333:RNA, Messenger / Q000378:metabolism*; D013379:Substrate Specificity; D013737:Testis / Q000378:metabolism*
D003432:Cross-Linking Reagents; D035683:MicroRNAs; D009419:Nerve Tissue Proteins; C429773:Ptbp2 protein, mouse; D012333:RNA, Messenger; D038941:Polypyrimidine Tract-Binding Protein