title: [The effect of HCPT combined with radiation on cell proliferation of human lung adenocarcinoma cell line (D₆) in vitro].
abstract: BACKGROUND
  To study the adjuvant effects of HCPT and radiation on cell proliferation of human lung adenocarcinoma cell line (D₆) in vitro.
  
  
  METHODS
  Suppression rate of cell growth,apoptosis and cell cycle of human pulmonary adenocarcinoma cell line were detected by MTT assay,DNA agrose electrophoresis and flow-cytometry respectively.
  
  
  RESULTS
  The suppression effects of HCPT on cell proliferation were dose and time dependent.The cells were significantly blocked at S stage by HCPT.Apoptosis peak appeared at 24 and 48 hours after treatment with high concentration (50μg/ml) and low concentration of HCPT (1μg/ml) respectively.DNA "Ladder" started to appear at 48 hours by radiation alone,but DNA "Ladder" and apoptosis peak both appeared at 24-72 hours and 0-72 hours after treatment with HCPT alone and HCPT combined with radiation respectively.
  
  
  CONCLUSIONS
  HCPT combined with radiation may show the synergistic killing effect on human lung adenocarcinoma cell line (D₆) in vitro.
authors.affiliation: Department of Radiation Oncology,Zhejiang Cancer Hospital,Hangzhou,Zhejiang 310022,P.R.China.
authors.forename: S
authors.initials: S
authors.lastname: Ma
authors.forename: H
authors.initials: H
authors.lastname: Dai
authors.forename: X
authors.initials: X
authors.lastname: Chen
authors.forename: J
authors.initials: J
authors.lastname: Feng