[Bacterial 16S rRNA genes and expression of IL-1β, TNF-α and IgA in prostate tissues].
OBJECTIVE
To investigate the role of bacteria in the etiology of chronic prostatitis.


METHODS
Complete prostate specimens were obtained at autopsy from 192 organ donors (aged 20 - 38 years old) during 2002 to 2008 who died of non-prostatic diseases. One tissue taken from the peripheral prostatic zone according to McNeal was divided into two pieces. One piece of tissue was taken for routine pathological examinations and immunohistochemical studies of interleukin (IL)-1β, tumor necrosis factor-α (TNF-α) and IgA. Another one was taken for PCR assay to detect the bacterial 16S rRNA genes (16S rDNA).


RESULTS
Of 192 prostate specimens, 64 (33.3%) had pathological changes of chronic prostatitis and 38 (19.8%) specimens was positive for bacterial 16S rDNA. Positive rates of 16S rDNA in chronic prostatitis and non-prostatitis specimens were 50.0% (32/64) and 4.6% (6/128) respectively (χ(2) = 55.185, P < 0.001). Expressions of IL-1β, TNF-α and IgA in specimens of chronic prostatitis were significantly higher than those in non-prostatitis specimens (P < 0.001). A positive correlation could be found among three immunohistochemical indicators (P < 0.01). In 64 specimens with chronic prostatitis, a significant expression of IL-1β, TNF-α and IgA was more often demonstrated in 16S rDNA positive group than in 16S rDNA negative group (P < 0.001).


CONCLUSIONS
The up-regulations of bacterial 16S rDNA, cytokines and immunoglobulin A are involved in inflammatory response of chronic prostatitis. Bacterial infection may be an important cause of chronic prostatitis.
Xie
Hui
H
Department of Urology, First Affiliated Hospital, Wenzhou Medical College, Wenzhou 325000, China. xiehuiwenzhou@yahoo.com.cn
Chen
Hong-de
HD
Huang
Hui-cong
HC
Yang
Yi-rong
YR
Wu
Jian-bo
JB
He
Qiu-xiang
QX
Zhu
Qi-jian
QJ
Chen
Jian-ou
JO
Li
Cheng-di
CD
Xia
Peng
P
Shen
Long-jie
LJ
D000328:Adult; D001419:Bacteria / Q000235:genetics; D005798:Genes, Bacterial; D020459:Genes, rRNA; D006801:Humans; D007070:Immunoglobulin A / Q000378:metabolism*; D053583:Interleukin-1beta / Q000378:metabolism*; D008297:Male; D011467:Prostate / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*; D055815:Young Adult
D007070:Immunoglobulin A; D053583:Interleukin-1beta; D012336:RNA, Ribosomal, 16S; D014409:Tumor Necrosis Factor-alpha