Improved selective and differential medium for isolation of Escherichia coli O157:H7.
GMAC, a modified version of Sorbitol MacConkey medium (SMAC), was produced with a reduced quantity of selective agents and incorporated gentiobiose. GMAC supported a higher recovery rate of heat- or acid-injured Escherichia coli O157:H7 cells than SMAC with cefixime and tellurite (CT-SMAC), while differentiating E. coli O157:H7 from sorbitol-nonfermenting Hafnia alvei.
Park
Sang-Hyun
SH
Department of Food and Animal Biotechnology, Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, South Korea.
Ryu
Sangryeol
S
Kang
Dong-Hyun
DH
D003470:Culture Media / Q000737:chemistry*; D004927:Escherichia coli Infections / Q000382:microbiology*; D019453:Escherichia coli O157 / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification*; D020611:Hafnia alvei / Q000145:classification / Q000302:isolation & purification; D006801:Humans; D012641:Selection, Genetic
D003470:Culture Media