Chrysoeriol isolated from Eurya cilliata leaves protects MC3T3-E1 cells against hydrogen peroxide-induced inhibition of osteoblastic differentiation.
Chrysoeriol is a flavonoid compound found in several tropical medicinal plants. To elucidate the protective effects of chrysoeriol isolated from Eurya cilliata on the response of osteoblasts to oxidative stress, osteoblastic MC3T3-E1 cells were incubated with chrysoeriol and/or H₂O₂, and markers of osteoblast function and oxidative damage were examined. Chrysoeriol treatment significantly (P < 0.05) reversed the cytotoxic effect of H₂O₂ and increased collagen content, alkaline phosphatase activity and calcium deposition of osteoblasts in the presence of H₂O₂. These effects were blocked by ICI182780, suggesting that chrysoeriol's effect might be partly involved in estrogen action. Moreover, H₂O₂-induced reduction of osteocalcin was recovered in the presence of chrysoeriol. Chrysoeriol significantly (P < 0.05) decreased the production of receptor activator of nuclear factor-κB ligand, interleukin-6, protein carbonyl and malondialdehyde of MC3T3-E1 cells in the presence of H₂O₂. These results demonstrate that chrysoeriol isolated from E. cilliata can protect osteoblasts from oxidative stress-induced toxicity.
Kim
Young Ho
YH
College of Pharmacy, Chungnam National University, Daejeon 305-764, Korea.
Lee
Young Soon
YS
Choi
Eun Mi
EM
D016475:3T3 Cells; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology; D002470:Cell Survival; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism; D029791:Ericaceae / Q000737:chemistry*; D004958:Estradiol / Q000031:analogs & derivatives / Q000494:pharmacology; D004965:Estrogen Antagonists / Q000494:pharmacology; D047309:Flavones; D005419:Flavonoids / Q000302:isolation & purification* / Q000494:pharmacology*; D000077267:Fulvestrant; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D015850:Interleukin-6 / Q000032:analysis / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism; D015675:Osteocalcin / Q000032:analysis / Q000378:metabolism; D018384:Oxidative Stress; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D020011:Protective Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D053246:Receptor Activator of Nuclear Factor-kappa B / Q000032:analysis / Q000378:metabolism
D015415:Biomarkers; D004965:Estrogen Antagonists; D047309:Flavones; D005419:Flavonoids; D015850:Interleukin-6; D020011:Protective Agents; D053246:Receptor Activator of Nuclear Factor-kappa B; D015675:Osteocalcin; D000077267:Fulvestrant; D004958:Estradiol; D008315:Malondialdehyde; D003094:Collagen; D006861:Hydrogen Peroxide; C007054:chrysoeriol