Development and evaluation of a rapid influenza diagnostic test for the pandemic (H1N1) 2009 influenza virus.
We evaluated a new rapid influenza diagnostic test for the pandemic (H1N1) 2009 influenza virus by using real-time reverse transcription-PCR (rRT-PCR) and viral culture. The sensitivities were 68.5% and 64.5%, and the specificities were 98.4% and 97.6%, respectively. This kit should be used with caution, and negative results should be verified by a confirmative test.
Kwon
Donghyok
D
Division of Influenza Virus, Center for Infectious Diseases, National Institute of Health, Korea Centers for Disease Control and Prevention, 194 Tongil-Lo, Eunpyung-Gu, Seoul 122-701, Republic of Korea.
Shin
Kyeongcheol
K
Kwon
Mihwa
M
Oh
Hee-Bok
HB
Kang
Chun
C
Lee
Joo-Yeon
JY
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D007251:Influenza, Human / Q000175:diagnosis* / Q000821:virology; D008297:Male; D008875:Middle Aged; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D014773:Virology / Q000379:methods*; D014776:Virus Cultivation / Q000379:methods; D055815:Young Adult