The promoters of 3 celery salt-induced phloem-specific genes as new tools for monitoring salt stress responses.
Genes induced by a progressive 3 week salt stress (final NaCl concentration 300 mM) were identified in the phloem of celery (Apium graveolens L., cv Vert d'Elne). A subtractive library was constructed and screened for salt-induced, phloem-specific genes. Work was focused on phloem due to its central role in inter-organ exchanges. Three genes were studied in more details, 2 coding for metallothioneins (AgMT2 and AgMT3) and one for a new mannitol transporter (AgMaT3). Expression of a reporter gene in transgenic Arabidopsis under control of promoter of each gene was located in the phloem. pAgMT2 has a typical phloem pattern with slight induction by salt stress. pAgMT3 and pAgMaT3 expression was induced by salt stress, except in minor veins. pAgMaT3 was highly active in stressed roots. The promoters described here could be regarded as new tools for engineering salt-resistant plants.
Landouar-Arsivaud
Lucie
L
UMR-CNRS-UP 6503, LACCO - Laboratoire de Catalyse en Chimie Organique, Equipe Physiologie Moléculaire du Transport de Sucres, Université de Poitiers, Bâtiment Botanique, 40 Avenue du Recteur Pineau, 86022 Poitiers cedex, France.
Juchaux-Cachau
Marjorie
M
Jeauffre
Julien
J
Biolley
Jean-Philippe
JP
Maurousset
Laurence
L
Lemoine
Rémi
R
D000077102:Apium / Q000235:genetics* / Q000378:metabolism; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D017930:Genes, Reporter; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D026901:Membrane Transport Proteins; D008668:Metallothionein / Q000235:genetics / Q000378:metabolism; D052585:Phloem / Q000235:genetics* / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots; D030821:Plants, Genetically Modified / Q000378:metabolism; D011401:Promoter Regions, Genetic; D055049:Salt Tolerance / Q000235:genetics*; D055051:Salt-Tolerant Plants / Q000235:genetics* / Q000378:metabolism
C432563:AgMat1 protein, Apium graveolens; D002352:Carrier Proteins; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D010940:Plant Proteins; D008668:Metallothionein