A novel role of Dma1 in regulating forespore membrane assembly and sporulation in fission yeast.
In fission yeast Schizosaccharomyces pombe, a diploid mother cell differentiates into an ascus containing four haploid ascospores following meiotic nuclear divisions, through a process called sporulation. Several meiosis-specific proteins of fission yeast have been identified to play essential roles in meiotic progression and sporulation. We report here an unexpected function of mitotic spindle checkpoint protein Dma1 in proper spore formation. Consistent with its function in sporulation, expression of dma1(+) is up-regulated during meiosis I and II. We showed that Dma1 localizes to the SPB during meiosis and the maintenance of this localization at meiosis II depends on septation initiation network (SIN) scaffold proteins Sid4 and Cdc11. Cells lacking Dma1 display defects associated with sporulation but not nuclear division, leading frequently to formation of asci with fewer spores. Our genetic analyses support the notion that Dma1 functions in parallel with the meiosis-specific Sid2-related protein kinase Slk1/Mug27 and the SIN signaling during sporulation, possibly through regulating proper forespore membrane assembly. Our studies therefore revealed a novel function of Dma1 in regulating sporulation in fission yeast.
Li
Wen-zhu
WZ
School of Life Sciences, Xiamen University, Xiamen 361005, Fujian, China.
Yu
Zhi-yong
ZY
Ma
Peng-fei
PF
Wang
Yamei
Y
Jin
Quan-wen
QW
D018797:Cell Cycle Proteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D015966:Gene Expression Regulation, Fungal; D008540:Meiosis; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D009154:Mutation; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D021381:Protein Transport; D012568:Schizosaccharomyces / Q000166:cytology / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D008941:Spindle Apparatus / Q000235:genetics / Q000378:metabolism; D013172:Spores, Fungal / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D015854:Up-Regulation
C477369:CDC11 protein, S pombe; D018797:Cell Cycle Proteins; C103134:Dma1 protein, S pombe; D008869:Microtubule-Associated Proteins; D029702:Schizosaccharomyces pombe Proteins; C409024:Sid4 protein, S pombe; C530792:Mug27 protein, S pombe; D017346:Protein Serine-Threonine Kinases