Genotyping of Enterococcus faecalis and Enterococcus faecium isolates by use of a set of eight single nucleotide polymorphisms.
A single nucleotide polymorphism (SNP) genotyping method for Enterococcus faecalis and Enterococcus faecium was developed using the "Minimum SNPs" program. SNP sets were interrogated using allele-specific real-time PCR. SNP typing subdivided clonal complexes 2 and 9 of E. faecalis and 17 of E. faecium, members of which cause the majority of nosocomial infections globally.
Rathnayake
I U
IU
Cell and Molecular Biosciences, Faculty of Science and Technology, Queensland University of Technology, 2 George St., Brisbane, Queensland 4001, Australia.
Hargreaves
M
M
Huygens
F
F
D000483:Alleles; D015373:Bacterial Typing Techniques / Q000379:methods*; D017931:DNA Primers / Q000235:genetics; D013293:Enterococcus faecalis / Q000145:classification* / Q000235:genetics*; D016984:Enterococcus faecium / Q000145:classification* / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D020641:Polymorphism, Single Nucleotide
D017931:DNA Primers