PEGylated guanidinylated polyallylamine as gene-delivery carrier.
A novel cationic co-polymer was developed by grafting poly(ethylene glycol) (PEG) on guanidinylated polyallylamine (PAA) for gene delivery. Characterization of PEG-g-guanidinylated PAA/DNA complexes demonstrated that particle size increased and surface charge decreased with increasing the amount of PEG. The results of cytotoxicity assay proved that grafted PEG could effectively decrease the cytotoxicity of the complexes. In transfection efficiency assay, HeLa cells treated with PEG(2)-g-guanidinylated PAA (formed with 17.5 μmol guanidinylated PAA and 2 μmol PEG)/DNA (0.2 μg EGFP plasmid) complexes showed a very high level of EGFP expression. In conclusion, combination of guanidinylation and PEGylation could effectively decrease the cytotoxicity and significantly increase the transfection efficiency of PAA.
Chung
Yi-Chen
YC
Institute of Polymer Science and Engineering, College of Engineering, National Taiwan University, Taipei 106, Taiwan.
Wei
Ming-Feng
MF
Chang
Fu-Hsiung
FH
Young
Tai-Horng
TH
D004247:DNA / Q000737:chemistry; D015316:Genetic Therapy / Q000379:methods; D049452:Green Fluorescent Proteins / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D010316:Particle Size; D010957:Plasmids / Q000737:chemistry; D011073:Polyamines / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D014162:Transfection / Q000379:methods*
D011073:Polyamines; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; C063994:polyallylamine; D011092:Polyethylene Glycols; D004247:DNA