title: [Study of HBV-DNA in ovarian granulosa cells of hepatitis B carriers].
abstract: OBJECTIVE
  To study the infection status of hepatitis B virus in ovarian granulosa cells of female hepatitis B carriers.
  
  
  METHODS
  The follicular fluid of 6 female carriers of hepatitis B (HBsAg+, HBeAg+, HBcAb+) was used to extract granule cells and prepare smears. The samples were detected via in situ hybridization by digoxin-labeled HBV-DNA probe. The color reaction system of alkaline phosphates was used. And the positive hybridization signal was purple blue.
  
  
  RESULTS
  Three granulosa cell samples, located at the nuclei of granulosa cells, were HBV-DNA positive.
  
  
  CONCLUSIONS
  HBV DNA exists in the granulosa cells of hepatitis B patients. Thus HBV infection may be vertically transmitted to the offspring via the infected reproductive cells.
authors.affiliation: Reproductive Research Center, Peking University Third Hospital, Beijing 100191, China.
authors.forename: Shun-li
authors.initials: SL
authors.lastname: Mei
authors.forename: Jin
authors.initials: J
authors.lastname: Huang
authors.forename: Ping
authors.initials: P
authors.lastname: Liu
authors.forename: Xiao-wei
authors.initials: XW
authors.lastname: Zhang
authors.forename: Jie
authors.initials: J
authors.lastname: Qiao
authors.forename: Qiu-fang
authors.initials: QF
authors.lastname: Zhang
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000328:Adult; D002353:Carrier State / Q000821:virology*; D054885:Cumulus Cells / Q000821:virology*; D015342:DNA Probes; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006509:Hepatitis B / Q000821:virology*; D006515:Hepatitis B virus / Q000235:genetics; D006801:Humans; D017403:In Situ Hybridization
chemical_list: D015342:DNA Probes; D004279:DNA, Viral