Intrafollicular and serum levels of leptin during in vitro fertilization cycles: comparison between the effects of recombinant follicle-stimulating hormones and human menopausal gonadotrophin.
OBJECTIVE
To compare the effect of recombinant follicle-stimulating hormones (r-FSH) and human menopausal gonadotrophin (hMG) on leptin levels in serum and follicular fluid (FF) during in vitro fertilization IVF/ET treatment, and to investigate whether leptin levels in the follicular fluid and/or serum are correlated with IVF success.


METHODS
Sixty-three patients undergoing IVF cycle were subdivided into two groups. r-FSH was used to for controlled ovarian hyperstimulation in 29 patients (Group A) while, hMG was used in 34 patients (Group B). Our main outcomes were serum and FF leptin on the day of oocyte collection.


RESULTS
The two groups were comparable in age, body mass index (BMI), indications for IVF/ET, E2 level on human chorionic gonadotrophin day, number of retrieved oocytes, fertilization rate, number of transferred embryos and pregnancy rate. Serum and FF leptin levels were similar between the two study groups. Additionally, no correlation was found between levels of leptin in either serum or FF and cycle results such as: number of retrieved oocytes, fertilization rate and pregnancy rate.


CONCLUSIONS
r-FSH and hMG have been found to have comparable effects on leptin levels in the serum and the FF of patients undergoing IVF/ET. Additionally, leptin levels in both serum and FF on day of retrieval have no correlation to IVF/ET outcome.
Almog
Benny
B
The Sara Racine IVF Unit, Lis Maternity Hospital and Tel Aviv Sourasky Medical Center, affiliated to Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel. bennyalmo@gmail.com
Azem
Foad
F
Kapustiansky
Rita
R
Azolai
Joseph
J
Wagman
Israel
I
Levin
Ishai
I
Hauser
Ron
R
Pauzner
David
D
Lessing
Joseph B
JB
Amit
Ami
A
Gamzu
Ronni
R
D000328:Adult; D005260:Female; D005300:Fertility Agents, Female / Q000494:pharmacology*; D043373:Follicle Stimulating Hormone, Human / Q000494:pharmacology*; D015571:Follicular Fluid / Q000378:metabolism; D006801:Humans; D020738:Leptin / Q000097:blood*; D008596:Menotropins / Q000494:pharmacology*; D010062:Ovulation Induction / Q000379:methods*; D011247:Pregnancy; D018873:Pregnancy Rate; D011446:Prospective Studies
D005300:Fertility Agents, Female; D043373:Follicle Stimulating Hormone, Human; D020738:Leptin; D008596:Menotropins