title: Fluorescence-based assay to measure the real-time kinetics of nucleotide incorporation during transcription elongation.
authors.affiliation: Department of Biochemistry, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, 683 Hoes Lane, Piscataway, NJ 08854, USA.
authors.forename: Guo-Qing
authors.initials: GQ
authors.lastname: Tang
authors.forename: Vasanti S
authors.initials: VS
authors.lastname: Anand
authors.forename: Smita S
authors.initials: SS
authors.lastname: Patel
mesh_terms: D015075:2-Aminopurine / Q000737:chemistry*; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry / Q000378:metabolism*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D007700:Kinetics; D008163:Luminescent Measurements / Q000379:methods*; D009711:Nucleotides / Q000378:metabolism; D012313:RNA / Q000096:biosynthesis*; D014158:Transcription, Genetic
chemical_list: D005456:Fluorescent Dyes; D009711:Nucleotides; D015075:2-Aminopurine; D012313:RNA; D012321:DNA-Directed RNA Polymerases