title: Expression, purification and characterization of cloning-grade zinc finger nuclease.
authors.affiliation: Department of Molecular, Cellular and Developmental Biology, The University of Michigan, Ann Arbor, MI 48109, USA.
authors.forename: Andriy
authors.initials: A
authors.lastname: Tovkach
authors.forename: Vardit
authors.initials: V
authors.lastname: Zeevi
authors.forename: Tzvi
authors.initials: T
authors.lastname: Tzfira
mesh_terms: D001483:Base Sequence; D020134:Catalytic Domain; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D003001:Cloning, Molecular / Q000379:methods; D004270:DNA, Circular / Q000235:genetics; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D003851:Deoxyribonucleases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli; D008969:Molecular Sequence Data; D009532:Nickel / Q000737:chemistry; D010957:Plasmids / Q000235:genetics; D015202:Protein Engineering; D055550:Protein Stability; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D016415:Sequence Alignment; D016335:Zinc Fingers
chemical_list: D004270:DNA, Circular; D004268:DNA-Binding Proteins; D011994:Recombinant Proteins; D009532:Nickel; D003851:Deoxyribonucleases