Characterization of a dockerin-based affinity tag: application for purification of a broad variety of target proteins.
Demishtein
Alik
A
Department of Biological Chemistry, The Weizmann Institute of Science, Rehovot 76100, Israel.
Karpol
Alon
A
Barak
Yoav
Y
Lamed
Raphael
R
Bayer
Edward A
EA
D000345:Affinity Labels / Q000737:chemistry* / Q000378:metabolism; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000737:chemistry* / Q000235:genetics; D002846:Chromatography, Affinity / Q000379:methods*; D002868:Chromosomal Proteins, Non-Histone / Q000737:chemistry* / Q000235:genetics; D003001:Cloning, Molecular; D048013:Acetivibrio thermocellus / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D006639:Histidine / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification*; D013379:Substrate Specificity; D000097722:Cohesins
D000345:Affinity Labels; D001426:Bacterial Proteins; D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; D006639:Histidine; D011993:Recombinant Fusion Proteins; D000097722:Cohesins