title: Phosphotriesterase variants with high methylphosphonatase activity and strong negative trade-off against phosphotriesters.
authors.affiliation: MRC Centre for Protein Engineering, Hills Road, Cambridge CB20QH, UK. briseno@biologie.ens.fr
authors.forename: Luis
authors.initials: L
authors.lastname: Briseño-Roa
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Timperley
authors.forename: Andrew D
authors.initials: AD
authors.lastname: Griffiths
authors.forename: Alan R
authors.initials: AR
authors.lastname: Fersht
mesh_terms: D002619:Chemical Warfare Agents / Q000378:metabolism*; D002800:Cholinesterase Inhibitors / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D008958:Models, Molecular; D009154:Mutation; D009943:Organophosphorus Compounds / Q000378:metabolism*; D009946:Organothiophosphorus Compounds / Q000378:metabolism; D044345:Phosphoric Triester Hydrolases / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D011485:Protein Binding; D011487:Protein Conformation; D011549:Pseudomonas / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics; D013379:Substrate Specificity
chemical_list: D002619:Chemical Warfare Agents; D002800:Cholinesterase Inhibitors; D009943:Organophosphorus Compounds; D009946:Organothiophosphorus Compounds; C032627:methylphosphonic acid; D044345:Phosphoric Triester Hydrolases