Fluorescence-based assay to measure the real-time kinetics of nucleotide incorporation during transcription elongation.
Tang
Guo-Qing
GQ
Department of Biochemistry, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, 683 Hoes Lane, Piscataway, NJ 08854, USA.
Anand
Vasanti S
VS
Patel
Smita S
SS
D015075:2-Aminopurine / Q000737:chemistry*; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry / Q000378:metabolism*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D007700:Kinetics; D008163:Luminescent Measurements / Q000379:methods*; D009711:Nucleotides / Q000378:metabolism; D012313:RNA / Q000096:biosynthesis*; D014158:Transcription, Genetic
D005456:Fluorescent Dyes; D009711:Nucleotides; D015075:2-Aminopurine; D012313:RNA; D012321:DNA-Directed RNA Polymerases