A high-throughput screening strategy for nitrile-hydrolyzing enzymes based on ferric hydroxamate spectrophotometry.
He
Yu-Cai
YC
Changzhou University, People's Republic of China. yucaihe2007@yahoo.com.cn
Ma
Cui-Luan
CL
Xu
Jian-He
JH
Zhou
Li
L
D000421:Alcaligenes / Q000201:enzymology; D000619:Aminohydrolases / Q000302:isolation & purification / Q000378:metabolism*; D005290:Ferric Compounds / Q000032:analysis*; D057166:High-Throughput Screening Assays / Q000379:methods; D006836:Hydro-Lyases / Q000302:isolation & purification / Q000378:metabolism*; D006868:Hydrolysis; D006877:Hydroxamic Acids / Q000032:analysis*; D009570:Nitriles / Q000378:metabolism*; D012240:Rhodococcus / Q000201:enzymology; D013053:Spectrophotometry / Q000379:methods*
D005290:Ferric Compounds; D006877:Hydroxamic Acids; D009570:Nitriles; C074579:iron (III) hydroxamate; D000619:Aminohydrolases; C021867:nitrilase; D006836:Hydro-Lyases; C048831:nitrile hydratase