Capture of human monoclonal antibodies from a clarified cell culture supernatant by phenyl boronate chromatography.
Azevedo
Ana M
AM
IBB - Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Av. Rovisco Pais, 1049-001 Lisbon, Portugal. a.azevedo@ist.utl.pt
Gomes
A G
AG
Borlido
L
L
Santos
I F S
IF
Prazeres
D M F
DM
Aires-Barros
M R
MR
D000327:Adsorption; D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism / Q000493:pharmacokinetics; D000915:Antibody Affinity; D001897:Boronic Acids / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D016466:CHO Cells; D018929:Cell Culture Techniques; D002846:Chromatography, Affinity / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006224:Cricetinae; D003412:Cricetulus; D017077:Culture Media, Conditioned / Q000737:chemistry* / Q000378:metabolism; D005240:Feasibility Studies; D006801:Humans; D011485:Protein Binding; D013379:Substrate Specificity
D000911:Antibodies, Monoclonal; D001897:Boronic Acids; D017077:Culture Media, Conditioned; C010686:benzeneboronic acid