title: Restrained expression, a method to overproduce toxic membrane proteins by exploiting operator-repressor interactions.
authors.affiliation: Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907, USA.
authors.forename: Anoop
authors.initials: A
authors.lastname: Narayanan
authors.forename: Marc
authors.initials: M
authors.lastname: Ridilla
authors.forename: Dinesh A
authors.initials: DA
authors.lastname: Yernool
mesh_terms: D003001:Cloning, Molecular / Q000379:methods*; D012321:DNA-Directed RNA Polymerases / Q000096:biosynthesis / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000235:genetics; D056968:Lac Repressors / Q000235:genetics; D009875:Operator Regions, Genetic; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000235:genetics; D019831:Sodium-Calcium Exchanger / Q000096:biosynthesis* / Q000235:genetics; D014764:Viral Proteins / Q000096:biosynthesis / Q000235:genetics
chemical_list: D056968:Lac Repressors; D011993:Recombinant Fusion Proteins; D019831:Sodium-Calcium Exchanger; D014764:Viral Proteins; D049452:Green Fluorescent Proteins; C062210:bacteriophage T7 RNA polymerase; D012321:DNA-Directed RNA Polymerases