Evolution of I-SceI homing endonucleases with increased DNA recognition site specificity.
Joshi
Rakesh
R
Department of Biochemistry, Purdue University, West Lafayette, IN 47907, USA.
Ho
Kwok Ki
KK
Tenney
Kristen
K
Chen
Jui-Hui
JH
Golden
Barbara L
BL
Gimble
Frederick S
FS
D000595:Amino Acid Sequence; D019943:Amino Acid Substitution / Q000235:genetics; D018360:Crystallography, X-Ray; D004247:DNA / Q000378:metabolism*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D019020:Directed Molecular Evolution; D008958:Models, Molecular; D008969:Molecular Sequence Data; D050505:Mutant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D006180:Proton-Translocating ATPases; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D016415:Sequence Alignment; D013379:Substrate Specificity
D050505:Mutant Proteins; D029701:Saccharomyces cerevisiae Proteins; D004247:DNA; C037725:SCEI protein, S cerevisiae; D015252:Deoxyribonucleases, Type II Site-Specific; D006180:Proton-Translocating ATPases; C081687:VMA1 protein, S cerevisiae