Suppressive effect of pectic polysaccharides from Cucurbita pepo L. var. Styriaca on citric acid-induced cough reflex in guinea pigs.
Several water-soluble pectic polysaccharides were isolated from the pumpkin fruit biomass and characterized by composition, structural features and molecular properties. The pectic polysaccharides were tested for antitussive activity by studying the effects of citric acid-induced cough reflex in guinea pigs and reactivity of the airway smooth muscle in vivo conditions in comparison to the narcotic drug codeine. Oral administration of all pectic polysaccharides from pumpkin inhibited the number of coughs induced by citric acid in guinea pigs, but to various extents. The results indicated that the antitussive activity of the pectic polysaccharides is affected by their molecular and structural properties, whereby a synergistic action between the polysaccharide and non-carbohydrate components on the biological response has been suggested as well. The cough depressive efficacy of most of the tested polysaccharides was comparable and even higher than that of codeine. Moreover, the application of these polysaccharides provoked any side effects what is their advantage towards the conventional opioid-derived antitussive agents.
Department of Pharmacology, Jessenius Faculty of Medicine, Comenius University, Martin, Slovakia.
Gabriela
G
Nosáľová
Lubica
L
Prisenžňáková
Zuzana
Z
Košťálová
Anna
A
Ebringerová
Zdenka
Z
Hromádková
D000818:Animals; D000996:Antitussive Agents / Q000494:pharmacology / Q000627:therapeutic use*; D019343:Citric Acid; D003061:Codeine / Q000627:therapeutic use; D003371:Cough / Q000139:chemically induced / Q000517:prevention & control*; D028464:Cucurbita / Q000737:chemistry*; D004357:Drug Synergism; D005638:Fruit; D006168:Guinea Pigs; D009130:Muscle, Smooth / Q000187:drug effects; D010368:Pectins / Q000494:pharmacology / Q000627:therapeutic use*; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D012018:Reflex / Q000187:drug effects; D012137:Respiratory System / Q000187:drug effects*; D013329:Structure-Activity Relationship
D000996:Antitussive Agents; D010936:Plant Extracts; D019343:Citric Acid; D010368:Pectins; D003061:Codeine