Efficacy of combined or single use of Lactobacillus crispatus LT116 and L. johnsonii LT171 on broiler performance.
1. The objective of this research was to investigate the efficacy of combined or single use of Lactobacillus crispatus LT116 and Lactobacillus johnsonii LT171 on broiler performance. 2. A total of 320 one-d-old male Ross broiler chicks were allocated in 4 experimental treatments for 6 weeks. The experimental treatments received a maize-soybean meal basal diet that was supplemented as follows: 'control', with no other additions; 'LJ', 1 × 10(6) CFU of L. johnsonii LT171; 'LC', 1 × 10(6) CFU of L. crispatus LT116; and 'LCJ', 0·5 × 10(6) CFU of L. johnsonii LT171 + 0·5 × 10(6) CFU of L. crispatus LT116/g of the diet. A suspension of sheep red blood cells (SRBC) was injected into the breast of 8 birds from each treatment on d 14 and 30, and the antibody titre was measured on d 20, 26, 36 and 42. 3. Body weight was improved when compared with control for broilers fed diets supplemented with LCJ. The feed conversion ratio (FCR) decreased in LC and LCJ groups compared with control. The number of coliforms in the ileum of LJ, LC and LCJ birds was lower than that from the control birds. However, only the LCJ treatment significantly decreased the number of coliforms in the caecum. The LCJ group had greater villus height in the duodenum than the LC group, and both LCJ and LC groups showed increased villus height in the duodenum and jejunum relative to the control. Antibody titre against SRBC was higher for the LCJ group than for the LJ and control groups in terms of secondary immune response (mean of 36 and 42 d). 4. This study showed, compared with the control, that the combination of Lactobacillus spp. could positively affect body weight, coliform numbers in the caecum and immune response.
Department of Animal Science, University College of Agricultural and Natural Resources, University of Tehran, Karaj, Iran. taherihr@gmail.com
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Taheri
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Moravej
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Tabandeh
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Zaghari
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Shivazad
D000818:Animals; D000917:Antibody Formation / Q000187:drug effects; D002432:Cecum / Q000187:drug effects / Q000382:microbiology; D002645:Chickens / Q000033:anatomy & histology / Q000276:immunology / Q000382:microbiology / Q000502:physiology*; D004386:Duodenum / Q000033:anatomy & histology / Q000187:drug effects / Q000382:microbiology; D004755:Enterobacteriaceae / Q000302:isolation & purification; D004912:Erythrocytes / Q000276:immunology; D007082:Ileum / Q000187:drug effects / Q000382:microbiology; D007583:Jejunum / Q000033:anatomy & histology / Q000187:drug effects / Q000382:microbiology; D007778:Lactobacillus; D008297:Male; D019936:Probiotics / Q000008:administration & dosage / Q000494:pharmacology*; D012756:Sheep / Q000097:blood