Identification of functional domains and novel binding partners of STIM proteins.
With a signal trap method, we previously identified stromal interaction molecule (STIM: originally named as SIM) as a protein, which has a signal peptide in 1996. However, recent works have accumulated evidences that STIM1 and STIM2 reside in endoplasmic reticulum (ER) and that both mainly sense ER Ca(2+) depletion, which plays an essential role in store operated calcium entry. In the present study, we extensively analyzed the domain functions and associated molecules of STIMs. A STIM1 mutant lacking the coiled-coil domains was massively expressed on the cell surface while mutants with the coiled-coil domains localized in ER. In addition, STIM1 mutants with the coiled-coil domains showed a longer half-life of proteins than those without them. These results are likely to indicate that the coiled-coil domains of STIM1 are essential for its ER-retention and its stability. Furthermore, we tried to comprehensively identify STIM1-associated molecules with mass spectrometry analysis of co-immunoprecipitated proteins for STIM1. This screening clarified that both STIM1 and STIM2 have a capacity to bind to a chaperone, calnexin as well as two protein-transporters, exportin1 and transportin1. Of importance, our result that glycosylation on STIM1 was not required for the association between STIM1 and calnexin seems to indicate that calnexin might function on STIM1 beyond a chaperone protein. Further information concerning regulatory mechanisms for STIM proteins including the data shown here will provide a model of Ca(2+) control as well as a useful strategy to develop therapeutic drugs for intracellular Ca(2+)-related diseases including inflammation and allergy.
Department of Hematology and Oncology, Graduate School of Medicine, Osaka University, Osaka, Japan.
Norimitsu
N
Saitoh
Kenji
K
Oritani
Kazunobu
K
Saito
Takafumi
T
Yokota
Michiko
M
Ichii
Takao
T
Sudo
Natsuko
N
Fujita
Koichi
K
Nakajima
Masato
M
Okada
Yuzuru
Y
Kanakura
D000818:Animals; D001665:Binding Sites; D015220:Calcium Channels; D037281:Calnexin / Q000378:metabolism; D045744:Cell Line, Tumor; D004721:Endoplasmic Reticulum / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D000071737:Stromal Interaction Molecule 1; D000071738:Stromal Interaction Molecule 2; D014162:Transfection; D028961:beta Karyopherins / Q000378:metabolism
D015220:Calcium Channels; D008562:Membrane Glycoproteins; C100757:Stim1 protein, mouse; C525024:Stim2 protein, mouse; D000071737:Stromal Interaction Molecule 1; D000071738:Stromal Interaction Molecule 2; D028961:beta Karyopherins; D037281:Calnexin