Detection of the CS20 colonization factor antigen in diffuse-adhering Escherichia coli strains.
We analyzed a randomly selected group of 30 diffusely adherent (DAEC), 30 enteropathogenic, 30 enteroaggregative, and five Shiga toxin-producing Escherichia coli strains isolated from children with diarrhea. Enterotoxigenic E. coli (ETEC) colonization factors (CFs) were evaluated by a dot-blot assay using 21 CF-specific monoclonal antibodies. Out of 95 non-ETEC strains, three DAEC were found to express coli surface antigen 20 (CS20). No other E. coli expressed CFs. We confirmed the three CS20-positive strains as ETEC-negative by repeat PCR and as toxin-negative by ganglioside-GM1-enzyme-linked immunosorbent assay. To our knowledge, this is the first study that has identified currently recognized CFs in non-ETEC diarrheagenic E. coli strains identified using molecular methods. CFs may be an unrecognized relevant adherence factor in other E. coli, which may then play a role in pathogenesis and the immune response of the host.
Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia, Lima, Peru. theresa.j.ochoa@uth.tmc.edu
Theresa J
TJ
Ochoa
Fulton P
FP
Rivera
Maria
M
Bernal
Rina
R
Meza
Lucie
L
Ecker
Ana I
AI
Gil
David
D
Cepeda
Susan
S
Mosquito
Erik
E
Mercado
Ryan C
RC
Maves
Eric R
ER
Hall
Ann-Mari
AM
Svennerholm
Annette
A
McVeigh
Stephen
S
Savarino
Claudio F
CF
Lanata
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial / Q000032:analysis / Q000276:immunology; D000954:Antigens, Surface / Q000032:analysis / Q000276:immunology; D001422:Bacterial Adhesion; D002648:Child; D003967:Diarrhea / Q000382:microbiology; D054308:Enteropathogenic Escherichia coli / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000235:genetics / Q000276:immunology* / Q000378:metabolism / Q000472:pathogenicity; D004927:Escherichia coli Infections / Q000382:microbiology; D029968:Escherichia coli Proteins / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D036781:Fimbriae Proteins / Q000032:analysis*; D006801:Humans; D016133:Polymerase Chain Reaction; D054323:Shiga-Toxigenic Escherichia coli / Q000235:genetics / Q000276:immunology / Q000378:metabolism
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D000954:Antigens, Surface; D029968:Escherichia coli Proteins; C022924:colonization factor antigens; D036781:Fimbriae Proteins