A live attenuated Listeria monocytogenes vaccine vector expressing SIV Gag is safe and immunogenic in macaques and can be administered repeatedly.
Listeria monocytogenes (Lm) is known to induce strong cellular immune responses. We constructed a live-attenuated Lm vector, Lmdd-BdopSIVgag, which encodes SIVmac239 gag. Intragastric (i.g.) administration of 3 × 10(12) bacteria to rhesus macaques was safe and induced anti-Gag cellular but no humoral immune responses. Boosting of Gag-specific cellular responses was observed after i.g. administration of Lmdd-BdopSIVgag to previously vaccinated RM despite preexisting anti-Lm immunity shown by lymphoproliferative responses. Surprisingly, anti-Lm cellular responses were also detected in non-vaccinated controls, which may reflect the fact that Lm is a ubiquitous bacterium. The novel, live-attenuated Lmdd-BdopSIVgag may be an attractive platform for oral vaccine delivery.
Department of Cancer Immunology and AIDS, Dana-Farber Cancer Institute, Boston, MA 02115, USA.
Gaia
G
Sciaranghella
Samir K
SK
Lakhashe
Mila
M
Ayash-Rashkovsky
Saied
S
Mirshahidi
Nagadenahalli B
NB
Siddappa
Francis J
FJ
Novembre
Vijayakumar
V
Velu
Rama Rao
RR
Amara
Chenghui
C
Zhou
Sufen
S
Li
Zhongxia
Z
Li
Fred R
FR
Frankel
Ruth M
RM
Ruprecht
D000818:Animals; D004337:Drug Carriers; D015683:Gene Products, gag / Q000235:genetics / Q000276:immunology*; D005822:Genetic Vectors; D007111:Immunity, Cellular; D007117:Immunization, Secondary / Q000379:methods; D008089:Listeria monocytogenes / Q000235:genetics*; D008253:Macaca mulatta; D018449:SAIDS Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D015302:Simian Immunodeficiency Virus / Q000235:genetics / Q000276:immunology*; D014611:Vaccination / Q000379:methods; D014613:Vaccines, Attenuated / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology
D004337:Drug Carriers; D015683:Gene Products, gag; D018449:SAIDS Vaccines; D014613:Vaccines, Attenuated