The spatial-functional coupling of box C/D and C'/D' RNPs is an evolutionarily conserved feature of the eukaryotic box C/D snoRNP nucleotide modification complex.
Box C/D ribonucleoprotein particles guide the 2'-O-ribose methylation of target nucleotides in both archaeal and eukaryotic RNAs. These complexes contain two functional centers, assembled around the C/D and C'/D' motifs in the box C/D RNA. The C/D and C'/D' RNPs of the archaeal snoRNA-like RNP (sRNP) are spatially and functionally coupled. Here, we show that similar coupling also occurs in eukaryotic box C/D snoRNPs. The C/D RNP guided 2'-O-methylation when the C'/D' motif was either mutated or ablated. In contrast, the C'/D' RNP was inactive as an independent complex. Additional experiments demonstrated that the internal C'/D' RNP is spatially coupled to the terminal box C/D complex. Pulldown experiments also indicated that all four core proteins are independently recruited to the box C/D and C'/D' motifs. Therefore, the spatial-functional coupling of box C/D and C'/D' RNPs is an evolutionarily conserved feature of both archaeal and eukaryotic box C/D RNP complexes.
Department of Molecular and Structural Biochemistry, North Carolina State University, Raleigh, NC 27695-7622, USA. stu_maxwell@ncsu.edu
Guosheng
G
Qu
Rob W
RW
van Nues
Nicholas J
NJ
Watkins
E Stuart
ES
Maxwell
D019843:Archaeal Proteins / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D056890:Eukaryota / Q000235:genetics; D019143:Evolution, Molecular; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008745:Methylation; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009711:Nucleotides / Q000235:genetics / Q000378:metabolism; D019642:RNA, Archaeal / Q000235:genetics / Q000378:metabolism; D012261:Ribonucleoproteins / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D020718:Ribonucleoproteins, Small Nucleolar / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D058727:RNA, Small Untranslated
D019843:Archaeal Proteins; D005656:Fungal Proteins; D009711:Nucleotides; D019642:RNA, Archaeal; D012261:Ribonucleoproteins; D020718:Ribonucleoproteins, Small Nucleolar; D058727:RNA, Small Untranslated