An altered method of feeding RNAi that knocks down multiple genes simultaneously in the nematode Caenorhabditis elegans.
In reverse genetics, RNA interference (RNAi) which is substitutable for gene-disruption, is an outstanding method for knockdown of a gene's function. In Caenorhabditis elegans, feeding RNAi is most convenient, but this RNAi is not suitable for knockdown of multiple genes. Hence, we attempted to establish an efficient method of feeding RNAi for multiple knockdown. We produced bacteria yielding three distinct double-stranded RNAs bound to one another, and fed those bacteria to C. elegans. Quantitative RT-PCR and observation of phenotypes indicated that our method is much more efficient than the traditional one. Our method is useful for investigating genes' functions in C. elegans.
Department of Bioresource Sciences, Faculty of Agriculture, Tottori University, Koyama, Japan.
Kenji
K
Gouda
Yohei
Y
Matsunaga
Takashi
T
Iwasaki
Tsuyoshi
T
Kawano
D000818:Animals; D001419:Bacteria / Q000378:metabolism; D017173:Caenorhabditis elegans / Q000235:genetics*; D055785:Gene Knockdown Techniques / Q000379:methods*; D008722:Methods; D009348:Nematoda; D012329:RNA, Bacterial; D034741:RNA, Small Interfering / Q000008:administration & dosage* / Q000494:pharmacology
D012329:RNA, Bacterial; D034741:RNA, Small Interfering