Differentiation of mouse embryonic stem cells in self-assembling peptide scaffolds.
Here, we describe the capacity of mouse embryonic stem cells (mESCs) to differentiate into osteoblast-like cells in a three-dimensional (3D) self-assembling peptide scaffold, a synthetic nanofiber biomaterial with future applications in regenerative medicine. We have previously demonstrated that classical tissue cultures (two-dimensional) as well as 3D-systems promoted differentiation of mESCs into cells with an osteoblast-like phenotype expressing osteopontin (OPN) and collagen type I (Col I), as well as high alkaline phosphatase (Alk Phos) activity and calcium phosphate mineralization. Interestingly, in 3D self-assembling peptide scaffold cultures, the frequency of appearance of embryonic stem-cell-like colonies was substantially enhanced, suggesting that this particular 3D microenvironment promoted the generation of a stem-cell-like niche that allows the maintenance of a small pool of undifferentiated cells. We propose that the 3D system provides a unique microenvironment permissive to promote differentiation of mESCs into osteoblast-like cells while maintaining its regenerative capacity.
Department of Bioengineering, Institut Químic de Sarrià Universitat Ramon Llull, Barcelona, Spain.
Núria
N
Marí-Buyé
Carlos E
CE
Semino
D000818:Animals; D015153:Blotting, Western; D002113:Calcification, Physiologic / Q000187:drug effects; D002118:Calcium / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation / Q000187:drug effects*; D002458:Cell Fractionation; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002469:Cell Separation; D024042:Collagen Type I / Q000378:metabolism; D050659:Core Binding Factor Alpha 1 Subunit / Q000378:metabolism; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D049452:Green Fluorescent Proteins / Q000378:metabolism; D007700:Kinetics; D051379:Mice; D050814:Octamer Transcription Factor-3 / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects; D053495:Osteopontin / Q000378:metabolism; D010455:Peptides / Q000494:pharmacology*; D012313:RNA / Q000302:isolation & purification; D013194:Staining and Labeling; D054457:Tissue Scaffolds / Q000737:chemistry*
D024042:Collagen Type I; D050659:Core Binding Factor Alpha 1 Subunit; D050814:Octamer Transcription Factor-3; D010455:Peptides; D053495:Osteopontin; D049452:Green Fluorescent Proteins; D012313:RNA; D002118:Calcium