Laminin-511 and integrin beta-1 in hair follicle development and basal cell carcinoma formation.
BACKGROUND
Initiation of the hair follicle placode and its subsequent growth, maturation and cycling in post-natal skin requires signaling interactions between epithelial cells and adjacent dermal cells and involves Shh signaling via the primary cilium. Previous reports have implicated laminins in hair follicle epithelial invagination.


RESULTS
Here we use a human BCC model system and mouse mutants to re-evaluate the role of laminin-511 in epithelial invagination in the skin. Blocking laminin 511 and 332 in BCCs maintains primary cilia and Shh signalling, but prevents invagination. Similarly, in laminin-511 and dermal beta-1 integrin mutants, dermal papilla development and primary cilia formation are normal. Dermal beta-1 integrin mutants have normal hair follicle development.


CONCLUSIONS
Our data provides support for a primary role of laminin-511 promoting hair follicle epithelial downgrowth without affecting dermal primary cilia and Shh target gene induction.
Cancer Biology Graduate Program, 251 Campus Drive, MSOB X234, Stanford, CA 94305-5173, USA
Mindy C
MC
DeRouen
Hanson
H
Zhen
Si Hui
SH
Tan
Samantha
S
Williams
M Peter
MP
Marinkovich
Anthony E
AE
Oro
D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002280:Basal Cell Carcinoma / Q000378:metabolism* / Q000473:pathology; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism; D018859:Hair Follicle / Q000196:embryology* / Q000254:growth & development* / Q000378:metabolism; D053823:Hedgehog Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D019012:Integrin beta1 / Q000235:genetics / Q000378:metabolism*; D007797:Laminin / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009024:Morphogenesis / Q000502:physiology; D009368:Neoplasm Transplantation; D015398:Signal Transduction / Q000502:physiology; D016038:Skin Transplantation; D014183:Transplantation, Heterologous; D000097794:Kalinin
D015415:Biomarkers; D015815:Cell Adhesion Molecules; D053823:Hedgehog Proteins; D019012:Integrin beta1; D007797:Laminin; D000097794:Kalinin; C509239:SHH protein, human; C097043:laminin alpha5; C551997:laminin-511, human