Metabolism mediated interaction of α-asarone and Acorus calamus with CYP3A4 and CYP2D6.
The present study was aimed to investigate the possible interaction of the standardized extract of Acorus calamus (AC) with Cytochrome P450 enzyme, quantitative determination of the α-asarone in the AC rhizome was performed by RP-HPLC method. In vitro interaction of the plant extract was evaluated by CYP450-carbon monoxide complex (CYP450-CO) assay. Effect on individual isoforms such as CYP3A4 and CYP2D6 isozymes were analyzed through fluorescence product formation and respective IC(50) values were determined. CYP450-CO assay showed moderate interaction potential. Extract showed higher IC(50) values (46.84±1.83-32.99±2.21 μg/ml) comparing to the standard inhibitors and lower IC(50) value than α-asarone (65.16±2.37-42.15±2.45 μg/ml).
School of Natural Product Studies, Department of Pharmaceutical Technology, Jadavpur University, Kolkata, India.
Subrata
S
Pandit
Pulok K
PK
Mukherjee
Sivasankaran
S
Ponnusankar
Murugan
M
Venkatesh
N
N
Srikanth
D029023:Acorus / Q000737:chemistry*; D000081248:Allylbenzene Derivatives; D000818:Animals; D000840:Anisoles / Q000494:pharmacology*; D019389:Cytochrome P-450 CYP2D6 / Q000378:metabolism; D051544:Cytochrome P-450 CYP3A / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D041743:Herb-Drug Interactions; D020128:Inhibitory Concentration 50; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D027343:Rhizome
D000081248:Allylbenzene Derivatives; D000840:Anisoles; D010936:Plant Extracts; C012195:asarone; D003577:Cytochrome P-450 Enzyme System; D019389:Cytochrome P-450 CYP2D6; D051544:Cytochrome P-450 CYP3A; C510163:CYP3A4 protein, human