In vitro interactions of Fusarium and Acanthamoeba with drying residues of multipurpose contact lens solutions.
OBJECTIVE
To examine in vitro effects of evaporation and drying of multipurpose contact lens solutions on survival of Fusarium and Acanthamoeba.


METHODS
Conidia of representative Fusarium from the 2004-2006 keratitis outbreak and trophozoites of Acanthamoeba castellanii were inoculated into commercially available multipurpose contact lens care solutions. These solutions were inoculated with 10(2)-10(6) microbial propagules/mL and were evaporated for at least 24 hours. After drying, nutrient media for recovery of surviving organisms were added to the residues formed in the lids of 38 mm polystyrene Petri dishes. General morphologic patterns of the solution residuals and the distribution and morphologies of the microorganisms were recorded with microscopic imaging.


RESULTS
Various multipurpose contact lens disinfection solutions formed distinctive dried residual patterns. Both Fusarium and Acanthamoeba at concentrations tested above 10(3) per mL of disinfection solution were recovered from dried films with replicate testing. Mature cysts of Acanthamoeba not evident in the inocula were observed in sparse numbers in all dried solutions except one (Complete Moisture Plus; Advanced Medical Optics) and control salines where precysts and mature cysts were common. Both fusaria and amoeba tended to be observed in discrete regions of the dried residues.


CONCLUSIONS
Regions of drying films of multipurpose contact lens disinfection solutions on contact lens cases may induce and harbor dormant-resistant stages of Fusarium and Acanthamoeba. It is hypothesized that the evaporation and drying of multipurpose contact lens disinfection solutions may have been an added risk factor for case contamination among Fusarium and Acanthamoeba keratitis patients. The need for frequent replacement of contact lens cases is enforced.
Biology Department, Georgia State University, Atlanta, Georgia, USA. dgahearn@att.net
Donald G
DG
Ahearn
Shangtong
S
Zhang
R Doyle
RD
Stulting
Robert B
RB
Simmons
Michael A
MA
Ward
George E
GE
Pierce
Sidney A
SA
Crow
D048248:Acanthamoeba castellanii / Q000187:drug effects* / Q000254:growth & development / Q000302:isolation & purification; D015169:Colony Count, Microbial; D018639:Contact Lens Solutions / Q000737:chemistry / Q000494:pharmacology*; D003890:Desiccation; D005670:Fusarium / Q000187:drug effects* / Q000254:growth & development / Q000302:isolation & purification; D066298:In Vitro Techniques; D012307:Risk Factors
D018639:Contact Lens Solutions