Use of primary culture of human fibroblasts in gingiva augmentation procedure.
BACKGROUND
The aim of this study was to assess gingival aesthetic after usage of an own method of primary culture of human fibroblasts derived from the connective tissue of oral cavity keratinized gingiva on collagenous carrier in gingival augmentation procedures.


METHODS
Procedures were performed on 10 patients (7 females, 3 males) aged 18-35 years. In total, 34 teeth in the anterior part of the maxilla and the mandible underwent treatment. The protocol consisted of (1) preparing the patient for tissue biopsy, (2) biopsy of keratinized tissue, (3) laboratory tissue culture, (4) application of expanded cells into the recipient site, and (5) post-procedure management. Aesthetic index, pocket depth (PD), and plaque (PI1) and after surgery complications were examined.


RESULTS
There was post-procedure aesthetic improvement in all 34 cases compared with the pre-procedure condition (grade 1), and furthermore a significant decrease in PD and PI1 were revealed.


CONCLUSIONS
Use of own method of primary culture of human fibroblasts on a collagenous carrier for gingival augmentation is an aesthetic method of mucogingival complex repair.
Department of Dental Surgery, Silesian Piast Medical University, Wrocław, Poland.
Marzena
M
Dominiak
Jolanta
J
Saczko
Hanna
H
Gerber
Zbigniew
Z
Rybak
Tomasz
T
Gredes
D000293:Adolescent; D000328:Adult; D002478:Cells, Cultured; D005260:Female; D005347:Fibroblasts / Q000637:transplantation*; D016557:Guided Tissue Regeneration, Periodontal / Q000295:instrumentation* / Q000379:methods*; D006801:Humans; D008297:Male; D008437:Maxilla / Q000000981:diagnostic imaging* / Q000601:surgery*; D011859:Radiography; D016896:Treatment Outcome; D055815:Young Adult