[Simultaneous determination of multicomponent in Xuefuzhuyu granule with different UV-wavelength by HPLC].
OBJECTIVE
To establish the method for the quality control of Xuefuzhuyu granule.


METHODS
The content of Semen Armeniacae Amarum PE, Hydroxysafflor yellow A, paeoniflorin and ferulic acid in Xuefuzhuyu granule were determined by HPLC under multi-wavelength of 210, 403, 230, 316 nm.


RESULTS
The calibration curve of Semen Armeniacae Amarum PE. hydroxysafflor yellow A, paeoniflorin and ferulic acid were linear within 0.1154-0.9232 (r = 0.9998), 0.0219-0.1754 (r = 0.9994), 0.3952-3.1616 (r = 0.9995), and 0.0426-0.3408 microg (r = 0.9998), respectively. The average recoveries were 101.78% (RSD was 1.7%), 99.60% (RSD was 2.9%), 98.90% (RSD was 2.0%), and 100.31% (RSD was 1.8%), respectively.


CONCLUSIONS
The method of quantification is accurate, rapid and reliable for the quality control of Xuefuzhuyu granule.
Tianjin High-Standard Quality Testing Lab., Tianjin 300081, China. liuzhenghui_2008@163.com
Zhenghui
Z
Liu
Tingxiang
T
Ye
Linlin
L
Zhao
Yi
Y
Cheng
Guoan
G
Luo
D001565:Benzoates / Q000032:analysis; D001952:Bridged-Ring Compounds / Q000032:analysis; D002599:Chalcone / Q000031:analogs & derivatives / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003373:Coumaric Acids / Q000032:analysis; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000737:chemistry*; D005960:Glucosides / Q000032:analysis; D039821:Monoterpenes; D011809:Quinones / Q000032:analysis; D015203:Reproducibility of Results; D006027:Glycosides
D001565:Benzoates; D001952:Bridged-Ring Compounds; D002599:Chalcone; D003373:Coumaric Acids; D004365:Drugs, Chinese Herbal; D005960:Glucosides; D039821:Monoterpenes; D011809:Quinones; C085278:hydroxysafflor yellow A; C015423:peoniflorin; D006027:Glycosides; C004999:ferulic acid