Vortex counter-current chromatography.
A novel counter-current chromatographic system is developed by mounting a vortex column on a type-I coil planet centrifuge. The column is fabricated from a high-density polyethylene disk (16 cm diameter and 5 cm thick) by making multiple holes of various diameters (3-12.5 mm) each arranged in a circle and connected with narrow transfer ducts. The performance of this vortex column is tested with three different two-phase solvent systems with a broad range in hydrophobicity. The results indicated that the smallest diameter column (3mm diameter, 120 units with 42.8 ml capacity) yielded the best separation with the height equivalent to a theoretical plate of 2 cm compared with 20 cm required by the conventional multilayer coil column of high-speed CCC. By avoiding the use of an Archimedean Screw Force, the system shows a low column pressure which would permit safe operation of a large preparative column without a risk of leakage of solvent and column damage.
Bioseparation Technology Laboratory, Biochemistry and Biophysics Center, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD, USA. itoy2@mail.nih.gov
Yoichiro
Y
Ito
Zhiyong
Z
Ma
Robert
R
Clary
Jimmie
J
Powell
Martha
M
Knight
Thomas M
TM
Finn
D003377:Countercurrent Distribution / Q000295:instrumentation* / Q000379:methods*; D004151:Dipeptides / Q000302:isolation & purification*
D004151:Dipeptides