[Differentiation of functional cells from iPS cells by efficient gene transfer].
Induced pluripotent stem (iPS) cells, which are generated from somatic cells by transducing four genes, are expected to have broad application to regenerative medicine. Although establishment of an efficient gene transfer system for iPS cells is considered to be essential for differentiating them into functional cells, the detailed transduction characteristics of iPS cells have not been examined. By using an adenovirus (Ad) vector containing the cytomegalovirus enhancer/beta-actin (CA) promoters, we have developed an efficient transduction system for mouse mesenchymal stem cells and embryonic stem (ES) cells. Also, we applied our transduction system to mouse iPS cells and investigated whether efficient differentiation could be achieved by Ad vector-mediated transduction of a functional gene. As in the case of ES cells, the Ad vector could efficiently transduce transgenes into mouse iPS cells. We found that the CA promoter had potent transduction ability in iPS cells. Moreover, exogenous expression of a PPARγ gene or a Runx2 gene into mouse iPS cells by an optimized Ad vector enhanced adipocyte or osteoblast differentiation, respectively. These results suggest that Ad vector-mediated transient transduction is sufficient to promote cellular differentiation and that our transduction methods would be useful for therapeutic applications based on iPS cells.
Laboratory of Stem Cell Regulation, National Institute of Biomedical Innovation, Osaka, Japan. kawabata@nibio.go.jp
Kenji
K
Kawabata
Katsuhisa
K
Tashiro
Hiroyuki
H
Mizuguchi
D000199:Actins; D000256:Adenoviridae; D017667:Adipocytes; D000818:Animals; D002454:Cell Differentiation; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics; D053595:Embryonic Stem Cells; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D057026:Induced Pluripotent Stem Cells / Q000166:cytology*; D059630:Mesenchymal Stem Cells; D051379:Mice; D010006:Osteoblasts; D047495:PPAR gamma / Q000235:genetics; D014161:Transduction, Genetic / Q000379:methods; D019076:Transgenes
D000199:Actins; D050659:Core Binding Factor Alpha 1 Subunit; D047495:PPAR gamma; C493716:Runx2 protein, mouse