Observation of a new pattern in serogroup-related PCR typing of Listeria monocytogenes 4b isolates.
Molecular serogroup-related PCR typing has made the determination of serotypes of Listeria monocytogenes isolates easy and rapid. Amplification of selected lineage- and serotype-related genes can produce serotype patterns reflecting the four major serotypes, 1/2a, 1/2b, 1/2c, and 4b. We found that four isolates in our routine testing had a pattern with the four bands lmo0737, ORF2110, ORF2819, and prs positive, a pattern which has not been previously reported in the literature. After testing with a lineage-specific PCR, hybridization, and conventional agglutination serotyping, the isolates with the new pattern were considered to be serotype 4b.
Public Health Microbiology Laboratory, Queensland Health Forensic and Scientific Services, 39 Kessels Road, Coopers Plains, Queensland 4108, Australia. Ben_Huang@health.qld.gov.au
Bixing
B
Huang
Ningxia
N
Fang
Karolina
K
Dimovski
Xian
X
Wang
Geoff
G
Hogg
John
J
Bates
D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D006801:Humans; D008089:Listeria monocytogenes / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D008088:Listeriosis / Q000382:microbiology*; D008969:Molecular Sequence Data; D058889:Molecular Typing; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial