[Inhibitory effect of total glucosides of paeonia on the NF-κB/p65 protein expression in paws of RA rats].
OBJECTIVE
To explore effects of total glucosides of paeonia (TGP)on the NF-κB/p65 protein expression in paws of rheumatoid arthritis (RA) rats, and the contents of serum TNF-α, IL-1β and IL-10 in RA rats serum.


METHODS
Type II collagen-induced arthritis model in SD rats was established. The expression of NF-κB/p65 proteins in rat, s paw tissues was detected by immunohistochemical staining. The contents of serum TNF-α, IL-1β and IL-10 in RA rats were detected by ELISA.


RESULTS
TGP could down-regulate the expression of NF-κB/ p65 protein and contents of TNF-α, IL-1β and IL-10 in RA rats.


CONCLUSIONS
The inhibitory effect of TGP on anti-inflammation maybe related to decreasing the expression of NF-κB/p65 protein and suppressing the production of TNF-α and IL-1β in RA rats.
Department of Biochemistry, Shangqiu Medical College, Shangqiu 476100, China. guoling_liu@126.com
Guo-ling
GL
Liu
Yi-chuan
YC
Li
Yong-jie
YJ
Shen
D000818:Animals; D001169:Arthritis, Experimental / Q000276:immunology / Q000378:metabolism; D001172:Arthritis, Rheumatoid / Q000378:metabolism*; D005260:Female; D005960:Glucosides / Q000494:pharmacology*; D016753:Interleukin-10 / Q000097:blood; D008297:Male; D029596:Paeonia / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D051996:Transcription Factor RelA / Q000032:analysis*; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
D005960:Glucosides; D051996:Transcription Factor RelA; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10