Effect of sophoridine on dextran sulfate sodium-induced colitis in C57BL/6 mice.
Sophoridine (SRI), one of the quinolizidine alkaloids, is a new anticancer drug with noticeable antitumor action and lower toxicity. To our knowledge, there is no report about its effect on colitis. Repeated colitis was induced by administration of four cycles of 4% DSS. The severity of colitis was assessed on the basis of clinical signs, colon length and histology scores. Moreover, cecum secretory immunoglobulin A (sIgA) and plasma haptoglobin (HP) were analyzed by enzyme-linked immunosorbent assay and ICAM-1, and macrophage migration inhibitory factor (MIF) gene expression was analyzed by quantitative reverse transcriptase real-time polymerase chain reaction using SYBR Green I. SRI administration significantly attenuated the damage and caused substantial reduction of the rise in plasma HP, and maintained the level of cecum sIgA. SRI inhibited the ICAM-1 gene expression and had no effect on MIF gene expression. In conclusion, for the first time, the activity of SRI on DSS-induced colitis mice was investigated, which suggests that SRI could be an attractive therapeutic option in the treatment of inflammatory bowel disease.
School of Pharmacy, Guangdong Medical College, Dongguan, China.
Wen-Chang
WC
Zhao
Li-Jun
LJ
Song
Hong-Zhu
HZ
Deng
D000470:Alkaloids / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D001483:Base Sequence; D003092:Colitis / Q000139:chemically induced* / Q000473:pathology; D016264:Dextran Sulfate / Q000494:pharmacology*; D004797:Enzyme-Linked Immunosorbent Assay; D006242:Haptoglobins / Q000032:analysis; D007071:Immunoglobulin A, Secretory / Q000032:analysis; D015212:Inflammatory Bowel Diseases; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis / Q000235:genetics; D007413:Intestinal Mucosa / Q000473:pathology / Q000648:ultrastructure; D008263:Macrophage Migration-Inhibitory Factors / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D015394:Molecular Structure; D011807:Quinolizines / Q000737:chemistry / Q000494:pharmacology*; D000093842:Matrines
D000470:Alkaloids; D006242:Haptoglobins; D007071:Immunoglobulin A, Secretory; D008263:Macrophage Migration-Inhibitory Factors; D011807:Quinolizines; D018799:Intercellular Adhesion Molecule-1; D016264:Dextran Sulfate; D000093842:Matrines