[The application of molecular differential diagnoses platform in identification of the respiratory pathogens].
OBJECTIVE
To establish a MDD (molecular differential diagnoses) platform for diagnosing the pathogens that may cause respiratory infection by combination of the advanced Tem-PCR(Target enriched multiplex PCR)with xMAP(multiple analyses profiling), and to evaluate the reliability and further use the platform to test clinic samples.


METHODS
22 throat swab specimen from outpatient patients of respiratory department in First Affiliated Hospital of Suzhou University and 20 respiratory tract lavage fluid specimen from inpatients of respiratory department in Affiliated Children Hospital of Suzhou University were collected, and the nucleic acids of the samples were amplified by Tem-PCR and xMAP.


RESULTS
Testing of the the known samples showed that the platform had excellent specificity and sensitivity. Testing of the clinic samples showed that the positive rate of the respiratory tract lavage fluid specimen was 63.6%, higher than that of the throat swab specimen, and that the positive rate of RNA pathogens was higher than that of DNA pathogens.


CONCLUSIONS
A reliable MDD platform for detection of respiratory pathogens has been established with high-throughput detection capacity.
Laoratory Department, Suzhou Center for Diseases Control and Prevention, Suzhou 215004, China.
Wei-ning
WN
Han
Zheng-ji
ZJ
Zhang
Xue-rong
XR
Ya
Wei
W
Ji
Wei
W
Heng
Yu
Y
Xia
Yuan-gen
YG
Xu
Zhi-yang
ZY
Shi
D057166:High-Throughput Screening Assays; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D010614:Pharynx / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012141:Respiratory Tract Infections / Q000175:diagnosis*; D012680:Sensitivity and Specificity