A phagocytotic inducer from herbal constituent, pentagalloylglucose enhances lipoplex-mediated gene transfection in dendritic cells.
Antigen-presenting cells are key vehicles for delivering antigens in tumor immunotherapy, and the most potent of them are dendritic cells (DCs). Recent studies have demonstrated the usefulness of DCs genetically modified by lipofection in tumor immune therapy, although sufficient gene transduction into DCs is quite difficult. Here, we show that Paeoniae radix, herbal medicine, and the constituent, 1,2,3,4,6-penta-O-galloyl-β-D-glucose (PGG), have an attractive function to enhance phagocytosis in murine dendritic cell lines, DC2.4 cells. In particular, PGG in combination with lipofectin (LPF) enhanced phagocytic activity. Furthermore, PGG enhanced lipofection efficacy in DC2.4 cells, but not in colorectal carcinoma cell lines, Colon26. In other words, PGG synergistically enhanced the effect of lipofectin-dependent phagocytosis on phagocytic cells. Hence, according to our data, PGG could be an effective aid in lipofection using dendritic cells. Furthermore, these findings provide an expectation that constituents from herbal plant enhance lipofection efficacy.
Division of Pathogenic Biochemistry, Institute of Natural Medicine, University of Toyama, 2630 Sugitani, Toyama, 930–0194, Japan.
Shinichiro
S
Kato
Keiichi
K
Koizumi
Miyuki
M
Yamada
Akiko
A
Inujima
Nobuhiro
N
Takeno
Tsuyoshi
T
Nakanishi
Hiroaki
H
Sakurai
Shinsaku
S
Nakagawa
Ikuo
I
Saiki
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D002460:Cell Line; D017024:Chemotherapy, Adjuvant; D003713:Dendritic Cells / Q000187:drug effects* / Q000378:metabolism; D047348:Hydrolyzable Tannins / Q000494:pharmacology*; D007167:Immunotherapy / Q000379:methods*; D008081:Liposomes; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009369:Neoplasms / Q000628:therapy; D029596:Paeonia / Q000737:chemistry*; D010587:Phagocytosis / Q000187:drug effects*; D010714:Phosphatidylethanolamines / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology; D018517:Plant Roots; D014162:Transfection / Q000379:methods*
D000972:Antineoplastic Agents, Phytogenic; D047348:Hydrolyzable Tannins; D008081:Liposomes; D010714:Phosphatidylethanolamines; D010936:Plant Extracts; C435084:pentagalloylglucose; C020888:1,2-dielaidoylphosphatidylethanolamine