An immunohistochemical study of the pancreatic endocrine cells of the ddN mouse.
The regional distribution and frequency of the pancreatic endocrine cells in the ddN mouse were studied using specific antisera against insulin, glucagon, somatostatin and human pancreatic polypeptide (hPP). In the pancreatic islets, most of insulin-immunoreactive (IR) cells were located in the central region, and glucagon-, somatostatin and hPP-IR cells were located in the peripheral region regardless of the lobe. In the splenic part, glucagon-IR cells were also located in the central regions, and more numerous somatostatin-IR cells were detected in the central regions as compared with the duo-denal part. hPP-IR cells were restricted to the peripheral regions in both lobes but more numerous cells were detected in the duodenal portion. In the exocrine parenchyma of the splenic lobe, only insulin- and glucagon-IR cells were detected but all four kinds of IR cells were observed in the duodenal portion. In addition, insulin and hPP-IR cells were also demonstrated in the pancreatic duct regions. In conclusion, some strain-dependent characteristic distributional patterns of pancreatic endocrine cells were found in the ddN mouse with somewhat different distributional patterns between the two pancreatic lobes.
Department of Clinical Laboratory Science, College of Health and Therapy, Daegu Haany University, Gyeongsan, Republic of Korea.
Hyeung Sik
HS
Lee
Jeong Hyun
JH
Chang
Sae Kwang
SK
Ku
D000818:Animals; D004386:Duodenum / Q000166:cytology / Q000378:metabolism; D004703:Endocrine System / Q000166:cytology* / Q000378:metabolism; D005934:Glucagon / Q000378:metabolism; D006801:Humans; D007004:Hypoglycemic Agents / Q000378:metabolism; D007106:Immune Sera / Q000276:immunology; D007150:Immunohistochemistry; D007328:Insulin / Q000378:metabolism; D007515:Islets of Langerhans / Q000166:cytology / Q000378:metabolism*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D010179:Pancreas / Q000166:cytology / Q000378:metabolism; D010183:Pancreatic Ducts / Q000166:cytology / Q000378:metabolism; D010191:Pancreatic Polypeptide / Q000378:metabolism; D011498:Protein Precursors / Q000378:metabolism; D013004:Somatostatin / Q000378:metabolism
D007004:Hypoglycemic Agents; D007106:Immune Sera; D007328:Insulin; C043710:PPY protein, human; D011498:Protein Precursors; D013004:Somatostatin; D010191:Pancreatic Polypeptide; D005934:Glucagon