Transcriptional activation of putative calmodulin genes am-cam-1 and am-cam-2 from Aquilaria microcarpa, in response to external stimuli.
A homology-based cloning strategy yielded two cDNA clones designated Am-cam-1 and Am-cam-2, presumably encoding calmodulin protein from a callus culture derived from the leaf tissues of Aquilaria microcarpa. An appreciable increase in the transcriptional activity of Am-cam-1 was reproducibly observed by exposure of the cell culture to methyl jasmonate, as analyzed by a reverse-transcription polymerase chain reaction. The expression level of the gene also increased when the cells were treated with yeast extract. The transcription of Am-cam-2 was similarly stimulated by the treatment with methyl jasmonate and yeast extract, however, the intensities of the enhanced expression appeared to be lower as compared with that of Am-cam-1. In contrast, Ca(2+)-ionophore A23187 did not show inducing activity for the expression of these two calmodulin genes. These results suggest that Am-cam-1 and Am-cam-2 and their products play important roles in signal transduction processes in methyl jasmonate- and yeast extract-treated cells of A. microcarpa, accompanying the change in the transcriptional activities.
Laboratory of Plant Resource Sciences, Graduate School of Medicine and Pharmaceutical Sciences for Research, University of Toyamam, Sugitani, Toyama 930–0194, Japan.
Yoshihide
Y
Kenmotsu
Yoshimi
Y
Yamamura
Shinjiro
S
Ogita
Yasuo
Y
Katoh
Fumiya
F
Kurosaki
D000085:Acetates / Q000378:metabolism* / Q000494:pharmacology; D000001:Calcimycin / Q000494:pharmacology; D002147:Calmodulin / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D003517:Cyclopentanes / Q000378:metabolism* / Q000494:pharmacology; D018076:DNA, Complementary; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant; D054883:Oxylipins / Q000378:metabolism* / Q000494:pharmacology; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology; D010937:Plant Growth Regulators / Q000378:metabolism / Q000494:pharmacology; D057865:Plant Immunity / Q000235:genetics*; D018515:Plant Leaves; D058439:Plant Somatic Embryogenesis Techniques; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D029645:Thymelaeaceae / Q000235:genetics / Q000378:metabolism*; D015533:Transcriptional Activation; D015003:Yeasts
D000085:Acetates; D002147:Calmodulin; D003517:Cyclopentanes; D018076:DNA, Complementary; D054883:Oxylipins; D010937:Plant Growth Regulators; D000001:Calcimycin; C072239:methyl jasmonate