Hydrolysis of timosaponin BII by the crude enzyme from Aspergillus niger AS 3.0739.
Timosaponin BII (1), a steroidal saponin showing potential anti-dementia activity, was regioselectively hydrolyzed into its deglycosyl derivatives by the crude enzyme from Aspergillus niger AS 3.0739. Three biotransformation products, timosaponin BII-a (2), timosaponin BII-b (3), and timosaponin BII-c (4), were purified and their structures were elucidated on the basis of 1D NMR, 2D NMR, FAB-MS, and HR-ESI-MS spectral data. Compounds 2 and 3 are new compounds.
Beijing Institute of Radiation Medicine, Beijing, China.
Wen-Bin
WB
Zhou
Bing
B
Feng
Hong-Zhi
HZ
Huang
Ping
P
Liu
He-Shui
HS
Yu
Yang
Y
Zhao
Yu-Juan
YJ
Qin
Li-Ping
LP
Kang
Bai-Ping
BP
Ma
D001234:Aspergillus niger / Q000201:enzymology*; D001711:Biotransformation; D003704:Dementia / Q000188:drug therapy; D006868:Hydrolysis; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D012503:Saponins / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D013237:Stereoisomerism; D013256:Steroids / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology
D012503:Saponins; D013256:Steroids; C523027:timosaponin B-II