Physiological and molecular characterization of atypical lipid-dependent Malassezia yeasts from a dog with skin lesions: adaptation to a new host?
Three lipid-dependent Malassezia isolates (here named 114A, 114B and 114C) recovered from a dog with skin lesions were phenotypically and genotypically characterized. All presented ovoid cells and buds formed on a narrow base. Most of the results from physiological tests were consistent with those of Malassezia furfur. The phylogenetic analysis of ITS-1 and LSU nucleotide sequences was concordant in placing all three clinical Malassezia isolates close to M. furfur. However, the phylogenetic data on the chs-2 sequence revealed that clinical isolate 114A is distinct from M. furfur and was closely affiliated to the sequence of M. pachydermatis with high nodal support. In particular, lipid-dependent isolates 114A displayed chs-2 sequences similar (100%) to that of the non-lipid dependent species Malassezia pachydermatis. The presence of the genetic and physiological polymorphisms detected in these three isolates of M. furfur could have resulted from a process of adaptation of this anthropophilic species to a new host.
Dipartimento di Sanità Pubblica e Zootecnia, Facoltà di Medicina Veterinaria, Valenzano, Bari, Italy.
C
C
Cafarchia
M S
MS
Latrofa
L A
LA
Figueredo
M L
ML
da Silva Machado
L
L
Ferreiro
J
J
Guillot
T
T
Boekhout
D
D
Otranto
D000220:Adaptation, Biological; D000595:Amino Acid Sequence; D000818:Animals; D002467:Cell Nucleus; D002687:Chitin Synthase / Q000235:genetics*; D004271:DNA, Fungal / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D003881:Dermatomycoses / Q000382:microbiology / Q000662:veterinary*; D004283:Dog Diseases / Q000382:microbiology*; D004285:Dogs; D005260:Female; D005800:Genes, Fungal; D005990:Glycerol / Q000031:analogs & derivatives; D008290:Malassezia / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D010802:Phylogeny
D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; C000515:cremophor EL; D002687:Chitin Synthase; C400821:chitin synthase 2; D005990:Glycerol