[Determination of chlorogenic acid and mangiferin in Folium Pyrrosiae from different habitats and species by HPLC].
An HPLC method for simultaneous determination of chlorogenic acid and mangiferin in original medicinal materials and decoction pieces of Pyrrosiae Folium was developed. The assay was performed on a Diamonsil C18 (4.6 mm x 250 mm, 5 microm) column eluted with a mobile phase consisted of acetonitrile and 0.5% phosphoric acid solution in gradient elution at a flow rate of 1.0 mL x min(-1). The column temperature was set at 25 degrees C. The detection wavelength was 320 nm. The results showed that The linear ranges of chlorogenic acid and mangiferin were 5.2-130 mg x L(-1) (r = 0.9999) and 1.2-18 microg x mL(-1) (r = 0.9999), and the average recoveries (n=6) were 97.9% (RSD 1.9%) and 99.6% (RSD 2.9%), respectively. The method was simple, reproducible and valid. It can be used for quality evaluation and control of original medicinal materials and decoction pieces of Pyrrosiae Folium.
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China.
Kaitong
K
Li
Yixuan
Y
Zhang
Yang
Y
Cao
Yue
Y
Shi
D002726:Chlorogenic Acid / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D010946:Plants, Medicinal / Q000737:chemistry*; D015203:Reproducibility of Results; D044004:Xanthones / Q000032:analysis*
D044004:Xanthones; C013592:mangiferin; D002726:Chlorogenic Acid