[Galactomannan from the seeds of Ural licorice (Glycyrrhiza uralensis Fisch.)].
Galactomannans from the seeds of Ural licorice (Glycyrrhiza uralensis Fisch.) obtained by hot water extraction of freshly ripened (GGu-1) and overwintered (GGu-2) seeds were studied. GGu-1 and GGu-2 (yield, 1.98 and 1.99% of the seed weight) had molecular weights of 1379 and 877 kDa, respectively; their solutions were characterized by high viscosity ([eta] 1193.1 and 765.8 mg/g, respectively) and optical activity ([alpha]D +64.8 and +65.6 deg, respectively). Their galactose-to-mannose ratio was 1 : 1.52 and 1 : 1.50, respectively. According to IR and 13C NMR spectroscopic data and methylation analysis, the polymeric chains of GGu-1 and GGu-2 are comprised of 1,4-beta-D-mannopyranose residues substituted at C-6 with single alpha-D-galactopyranose residues. The content of mannobiose units Man-Man, (Gal)Man-Man / Man-Man(Gal), and (Gal)Man-Man(Gal) differentially substituted with galactose in macromolecules GGu-1 and GGu-2 was 25.2, 18.4 and 55.9% for GGu-1 and 26.5, 32.5, and 41.0% for GGu-2.
D N
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Olennikov
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Rokhin
D005690:Galactose / Q000032:analysis / Q000737:chemistry / Q000378:metabolism / Q000031:analogs & derivatives; D029882:Glycyrrhiza uralensis / Q000737:chemistry* / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008351:Mannans / Q000032:analysis* / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D008358:Mannose / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D008970:Molecular Weight; D012639:Seeds / Q000737:chemistry* / Q000378:metabolism
D008351:Mannans; C012990:galactomannan; D008358:Mannose; D005690:Galactose