100% complete assignment of non-labile (1)H, (13)C, and (15)N signals for calcium-loaded Calbindin D(9k) P43G.
Here we present the 100% complete assignment chemical shift of non-labile (1)H, (15)N and (13)C nuclei of Calbindin D(9k) P43G. The assignment includes all non-exchangeable side chain nuclei, including ones that are rarely reported, such as LysNζ as well as the termini. NMR experiments required to achieve truly complete assignments are discussed. To the best of our knowledge our assignments for Calbindin D(9k) extend beyond previous studies reaching near-completeness (Vis et al. in Biochem 33:14858-14870, 1994; Yamazaki et al. in J Am Chem Soc 116:6464-6465, 1994; Yamazaki et al. in Biochem 32:5656-5669, 1993b).
Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, The Netherlands.
Nur Alia
NA
Oktaviani
Renee
R
Otten
Klaas
K
Dijkstra
Ruud M
RM
Scheek
Eva
E
Thulin
Mikael
M
Akke
Frans A A
FA
Mulder
D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000818:Animals; D064026:Calbindins; D002118:Calcium / Q000378:metabolism*; D002247:Carbon Isotopes; D002417:Cattle; D006859:Hydrogen; D008969:Molecular Sequence Data; D009587:Nitrogen Isotopes; D019906:Nuclear Magnetic Resonance, Biomolecular; D064030:S100 Calcium Binding Protein G / Q000737:chemistry*
D064026:Calbindins; D002247:Carbon Isotopes; D009587:Nitrogen Isotopes; D064030:S100 Calcium Binding Protein G; D006859:Hydrogen; D002118:Calcium