A novel method for the genome-wide high resolution analysis of DNA damage.
DNA damage occurs via endogenous and exogenous genotoxic agents and compromises a genome's integrity. Knowing where damage occurs within a genome is crucial to understanding the repair mechanisms which protect this integrity. This paper describes a new development based on microarray technology which uses ultraviolet light induced DNA damage as a paradigm to determine the position and frequency of DNA damage and its subsequent repair throughout the entire yeast genome.
Department of Medical Genetics, Haematology and Pathology, School of Medicine, Cardiff University, Heath Park, Cardiff, UK.
Yumin
Y
Teng
Mark
M
Bennett
Katie E
KE
Evans
Huayun
H
Zhuang-Jackson
Andy
A
Higgs
Simon H
SH
Reed
Raymond
R
Waters
D004249:DNA Damage; D004260:DNA Repair; D004268:DNA-Binding Proteins / Q000235:genetics; D016681:Genome, Fungal; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D011740:Pyrimidine Dimers / Q000032:analysis; D014466:Ultraviolet Rays; D015003:Yeasts / Q000235:genetics
D004268:DNA-Binding Proteins; D011740:Pyrimidine Dimers