[Simultaneous determination of berberine, palmatine, matrine, catechin and baicalin in Funing Shuan by micellar electrokinetic capillary chromatography-electrospray ionization mass spectrometry].
A method for the simultaneous determination of berberine, palmatine, matrine, catechin and baicalin in Funing Shuan was established using micellar electrokinetic capillary chromatography-electrospray ionization mass spectrometry (MEKC-ESI MS). The compounds were separated on an uncoated capillary (80 cm x 50 microm) with the operating voltage of 25 kV and the running buffer of 40 mmol/L lauric acid-100 mmol/L ammonia mixture containing 25% acetonitrile (pH 9.5). The baseline separation of the five compounds was achieved within 16 min with a satisfactory repeatability and sensitivity. The solution of 50% 2-propanol/water solution (containing 3 mmol/L acetic acid) was used as the sheath liquid for the ESI MS analysis. The results showed that the linear ranges for berberine, palmatine, matrine, catechin and baicalin were 0.03 - 15, 0.05 - 15, 0.2 - 250, 1.5 - 300 and 2.0 - 500 mg/L, respectively, and the detection limits were 0.01, 0.02, 0.05, 0.5 and 0.6 mg/L, respectively. The average recoveries of the five components were between 94.0% - 104.0% with the relative standard deviations (RSDs) of 0.3% - 3.2%. The developed method is simple, rapid, and accurate, and it is suitable for the routine analysis of the five effective components in Funing Shuan.
Guangxi Key Laboratory of Subtropical Bioresource Conservation and Utilization, Nanning 530004, China.
Yongfang
Y
Zeng
Peng
P
Huo
Yuanjin
Y
Xu
D000470:Alkaloids / Q000032:analysis; D001599:Berberine / Q000032:analysis*; D001600:Berberine Alkaloids / Q000032:analysis*; D053801:Capillary Electrochromatography / Q000379:methods*; D002392:Catechin / Q000032:analysis; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D005419:Flavonoids / Q000032:analysis; D006801:Humans; D011807:Quinolizines / Q000032:analysis; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D000093842:Matrines
D000470:Alkaloids; D001600:Berberine Alkaloids; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D011807:Quinolizines; D001599:Berberine; C038044:baicalin; D002392:Catechin; C005413:palmatine; D000093842:Matrines