Polymorphism of prion protein gene in sheep of Inner Mongolian, China.
Susceptibility to natural scrapie in sheep is associated with polymorphisms at codons 136, 154 and 171 of the prion protein (PrP) gene. To assess the risk of scrapie in sheep raised in China, DNA from 30 sheep of two breeds was isolated, amplified and sequenced for the PrP gene. The ovine PrP gene was found to be highly homogenous. The genotype associated with high susceptibility to scrapie (VRQ) was absent, whereas that associated with the resistance (ARR) was present in 6.7% of sheep examined. ARK was also rare (6.7%). ARQ that is associated with an intermediate susceptibility was the genotype observed in the most of sheep examined (86.6%). These data suggest that Chinese sheep of Mongolian sheep breed are susceptible to scrapie.
College of Veterinary Medicine, Northeast Agricultural University, Harbin, People's Republic of China.
Cai-Xia
CX
Han
Hong-Xiang
HX
Liu
Yi-Xin
YX
Lu
Ming-Xin
MX
Song
De-Ming
DM
Zhao
Xiang-Mei
XM
Zhou
Li-Feng
LF
Yang
Xiao-Yun
XY
Li
D000818:Animals; D002681:China; D003062:Codon; D004198:Disease Susceptibility / Q000662:veterinary*; D005787:Gene Frequency; D011110:Polymorphism, Genetic; D017096:Prion Diseases / Q000235:genetics / Q000662:veterinary*; D011328:Prions / Q000235:genetics*; D012608:Scrapie / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000662:veterinary*; D012756:Sheep / Q000821:virology*
D003062:Codon; D011328:Prions