[Production of cytokines by murine bone marrow dendritic cells in vitro mediated by sulfated polysaccharides obtained from sea brown algae].
OBJECTIVE
To assess in vitro cytokine production by murine bone marrow dendritic cells (DC) matured under the effect of sulfated polysaccharides--fucoidanes from sea brown algae Laminaria cichorioides and Laminaria japonica.


METHODS
CBA line mice were used to obtain bone marrow origin precursors of DC. Isolation and study of chemical composition and structure of fucoidanes were performed using modern research methods. Expression of surface markers was determined by flow cytometry (FACS-analysis) using monoclonal antibodies to respective antigens. Levels of cytokine production were measured by t-ELISA using kits manufactured by Biosource (Belgium).


RESULTS
I was determined that fucoidans induce maturation of DC that was evident by expression of terminal differentiation marker CD83, activation marker CD38, enhanced expression of costimulating CD86, antigen-presenting MHC II and adhesive CD11c molecules. Fucoidanes stimulate DC to produce proinflammatory (TNF-alpha, IL-6, IL-1beta) and regulatory (IL-12) cytokines. Fucoidanes enhance expression of TLR-2 and TLR-4 but do not influence on expression of TLR-9.


CONCLUSIONS
It was shown that fucoidanes from sea brown algae L. cichorioides and L. japonica activate innate immunity system that is evident by enhanced expression of surface molecules associated with DC maturation and increased production of proinflammatory and regulatory cytokines by DC. Enhanced expression of TLR-2 and TLR-4 allows to suppose that studied fucoidanes could have anti-infective effect in vivo.
I D
ID
Makarenkova
N K
NK
Akhmatova
I B
IB
Semenova
N N
NN
Besednova
T N
TN
Zviagintseva
N M
NM
Shevchenko
D051997:ADP-ribosyl Cyclase 1 / Q000096:biosynthesis; D000818:Animals; D015703:Antigens, CD / Q000096:biosynthesis; D001854:Bone Marrow Cells / Q000276:immunology* / Q000378:metabolism; D039521:CD11c Antigen / Q000096:biosynthesis; D002454:Cell Differentiation; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis*; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism; D005643:Fucose / Q000276:immunology*; D000949:Histocompatibility Antigens Class II / Q000096:biosynthesis; D007136:Immunoglobulins / Q000096:biosynthesis; D007795:Laminaria / Q000276:immunology*; D008297:Male; D008562:Membrane Glycoproteins / Q000096:biosynthesis; D051379:Mice; D008808:Mice, Inbred CBA; D011134:Polysaccharides / Q000276:immunology*; D000097802:CD83 Antigen
D051997:ADP-ribosyl Cyclase 1; D015703:Antigens, CD; D039521:CD11c Antigen; D016207:Cytokines; D005643:Fucose; D000949:Histocompatibility Antigens Class II; D007136:Immunoglobulins; D008562:Membrane Glycoproteins; D011134:Polysaccharides; D000097802:CD83 Antigen; C007789:fucoidan