Proteomic analysis of functional dyspepsia in stressed rats treated with traditional Chinese medicine "Wei Kangning".
OBJECTIVE
Chinese traditional medical science is generally used as a therapeutic method against functional dyspepsia (FD) in China. Although great effort is made to understand the pharmaceutical mechanisms of Chinese traditional medicine, such as typical traditional Chinese medicine, Wei Kangning, there are still many mysteries to be uncovered.


METHODS
The model of FD was established by stimulating rats via tail damping and the rats were treated with traditional Chinese medicine, Wei Kangning. The proteins of the rat gastrointestinal tissues were extracted and run by 2-DE, then the differential proteins were identified using matrix-assisted laser desorption ionisation time-of-flight mass spectrometry and validated with Western blotting or fluorescent quantitation polymerase chain reaction.


RESULTS
A total of 228 unique proteins in FD model rats were detected with significant changes in their expression levels corresponding with traditional Chinese medicine, Wei Kangning, administration. Twenty-eight of these proteins were identified, which are involved in many biological functions, such as organism antioxidant enzymes, energy metabolism, glutathione S-transferase, pi2, superoxide dismutase 2 and alpha-enolase and so on.


CONCLUSIONS
These proteomic results presented therefore provide additional support to the hypothesis that glutathione S-transferase, pi2, superoxide dismutase 2, α-enolase and voltage-dependent anion channel are the targets of FD treated with traditional Chinese medicine, Wei Kangning.
Digestion Department of Beijing Xuanwu Traditional Chinese Medical Hospital, Beijing, China.
Wei
W
Wei
Xia
X
Li
Jianjun
J
Hao
Rong
R
Zhang
Jianli
J
Guo
Yiqiang
Y
Zong
Yanjun
Y
Lu
Shen
S
Qu
Jun
J
Tian
D000818:Animals; D015153:Blotting, Western; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004415:Dyspepsia / Q000188:drug therapy* / Q000209:etiology / Q000378:metabolism / Q000503:physiopathology; D015180:Electrophoresis, Gel, Two-Dimensional; D005753:Gastric Mucosa / Q000378:metabolism; D005769:Gastrointestinal Motility / Q000187:drug effects; D051549:Glutathione S-Transferase pi / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000187:drug effects* / Q000503:physiopathology; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D040901:Proteomics / Q000379:methods; D051381:Rats; D017208:Rats, Wistar; D060888:Real-Time Polymerase Chain Reaction; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013270:Stomach / Q000187:drug effects* / Q000503:physiopathology; D013315:Stress, Psychological / Q000150:complications*; D013482:Superoxide Dismutase / Q000378:metabolism; D050994:Voltage-Dependent Anion Channels / Q000187:drug effects / Q000378:metabolism; D000099156:Superoxide Dismutase 2
D004365:Drugs, Chinese Herbal; D051549:Glutathione S-Transferase pi; D010751:Phosphopyruvate Hydratase; D013482:Superoxide Dismutase; D050994:Voltage-Dependent Anion Channels; D000099156:Superoxide Dismutase 2; C550198:weikangning