Inactivation of nuclear factor-κB by citrus flavanone hesperidin contributes to apoptosis and chemo-sensitizing effect in Ramos cells.
Hesperidin, a flavonoid present in fruits and vegetables, possesses anti-inflammatory and chemopreventive effects. Some evidence implies that hesperidin may recruit Peroxisome Proliferator-Activated Receptor-gamma (PPARγ) to exert biological action. It has been shown also that inactivation of Nuclear Factor-kappaB is another mechanism of flavonoids' action. Therefore, we wished to investigate the role of these transcription factors in apoptosis induced by hesperidin in Ramos Burkitt's lymphoma cells. We found that incubation of Ramos cells with hesperidin abrogates constitutive and doxorubicin-induced NF-κB activation through inhibition of phosphorylation of its inhibitory subunits (IκB). Moreover, our results showed that incubation of Ramos cells with hesperidin resulted in inhibition of proliferation and apoptosis in a PPARγ-independent way. Our results demonstrated that hesperidin inhibits proliferation of Ramos cells and sensitizes them to doxorubicin-induced apoptosis through inhibition of both constitutive and doxorubicin-mediated NF-κB activation in a PPARγ-independent manner.
Institute and Faculty of Nutrition Sciences and Food Technology, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Maryam
M
Nazari
Asghar
A
Ghorbani
Azita
A
Hekmat-Doost
Mahmood
M
Jeddi-Tehrani
Hamid
H
Zand
D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism; D002051:Burkitt Lymphoma; D053148:Caspase 3 / Q000378:metabolism; D053453:Caspase 9 / Q000378:metabolism; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002957:Citrus; D004317:Doxorubicin / Q000494:pharmacology; D004789:Enzyme Activation; D006569:Hesperidin / Q000494:pharmacology*; D006801:Humans; D020672:I-kappa B Proteins / Q000378:metabolism; D016328:NF-kappa B / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D047495:PPAR gamma / Q000502:physiology; D010766:Phosphorylation
D000970:Antineoplastic Agents; D051017:Apoptosis Regulatory Proteins; D020672:I-kappa B Proteins; D016328:NF-kappa B; D047495:PPAR gamma; D004317:Doxorubicin; D006569:Hesperidin; D053148:Caspase 3; D053453:Caspase 9