[Determination of ascitic bacterial 16S rRNA gene in the rapid diagnosis of spontaneous bacterial peritonitis].
OBJECTIVE
To evaluate the value of ascitic bacterial 16S rRNA gene determination in the rapid diagnosis of spontaneous bacterial peritonitis (SBP).


METHODS
16S rRNA gene from bacterial DNA in ascites was determined by quantitative fluorescent polymerase chain reaction (PCR) in 76 patients with suspected SBP and 6 patients with non-infectious ascites. The results were compared with those obtained from bacterial culture.


RESULTS
The positive rate of SBP was 22.4% among patients detected with ascitic bacterial 16S rRNA gene determination-based quantitative fluorescent PCR, which was significantly higher than that (7.9%) in patients only received bacterial culture (P<0.05). In addition,in 6 patients with non-infectious ascites,both the 16S rRNA gene determination-based quantitative fluorescent PCR and bacterial culture showed negative results.


CONCLUSIONS
16S rRNA gene determination-based quantitative fluorescent PCR can be an effective tool for the rapid diagnosis of SBP. It is more sensitive than the bacterial culture.
The 4th Department of Internal Medicine, Hangzhou Sixth Hospital, Zhejiang University of Traditional Chinese Medicine, Hangzhou 310014, China. panhongying@sohu.com
Hong-ying
HY
Pan
Hong-yun
HY
Sun
Cui-rong
CR
Chen
Qun-wei
QW
Chen
Jing
J
Xu
Rong-xia
RX
Ye
Guo-qiang
GQ
Lou
De-rong
DR
Lu
D000328:Adult; D000368:Aged; D001202:Ascitic Fluid / Q000382:microbiology*; D001424:Bacterial Infections / Q000175:diagnosis*; D004269:DNA, Bacterial / Q000032:analysis; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010538:Peritonitis / Q000175:diagnosis* / Q000382:microbiology; D012336:RNA, Ribosomal, 16S
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S