Studying transcriptional interactions in single cells at sufficient resolution.
Our ability to dissect and understand the principles of gene regulatory circuits is partly limited by the resolution of our experimental assays. In this brief review, we discuss aspects of gene expression in microbial organisms apparent only when increasing the experimental resolution from populations to single cells and sub-cellular structures, from snap-shots to high-speed time-lapse movies and from molecular ensembles to single molecules.
Department of Cell and Molecular Biology, Science for Life Laboratory, Uppsala University, Husargatan 3, Uppsala, Sweden.
Mats
M
Wallden
Johan
J
Elf
D001419:Bacteria; D015870:Gene Expression; D053263:Gene Regulatory Networks; D057230:Limit of Detection; D044085:Microfluidics / Q000379:methods; D008856:Microscopy, Fluorescence; D018715:Microscopy, Video; D056004:Molecular Dynamics Simulation; D015335:Molecular Probes / Q000737:chemistry / Q000378:metabolism*; D012441:Saccharomyces cerevisiae; D059010:Single-Cell Analysis / Q000379:methods*
D015335:Molecular Probes