Gene expression profile in rat dorsal root ganglion following sciatic nerve injury and systemic neurotrophin-3 administration.
Following sciatic nerve transection in adult rats, a proportion of injured dorsal root ganglion (DRG) neurons die, through apoptosis, over the following 6 months. Previous studies showed that axotomy and neurotrophin-3 administration may have effects on expression of neurotrophins and their receptors in DRG. In the current study, the fourth and fifth lumbar DRGs of rats were examined 2 weeks after right sciatic nerve transection and ligation. The effects of axotomy and systemic NT-3 treatment on neuronal genes were investigated by microarray. The results demonstrated that bone morphogenetic protein (BMP) and Janus protein tyrosine kinase signaling pathways are induced in axotomized DRG, and PI-3 kinase and BMP pathways and genes controlling various cellular functions were induced after axotomy and NT-3 administration.
Division of Neurosurgery, Department of Surgery, National Taiwan University Hospital, Yun-lin branch, No.579, Sec. 2, Yun-lin Rd., Dou-liou City, Yun-lin County, 640, Taiwan. kuoluting@yahoo.com.tw
Lu-Ting
LT
Kuo
Shao-Yu
SY
Tsai
Michael J
MJ
Groves
Shu F
SF
An
Francesco
F
Scaravilli
D000818:Animals; D005727:Ganglia, Spinal / Q000187:drug effects* / Q000502:physiology*; D020869:Gene Expression Profiling; D008297:Male; D046228:Microarray Analysis; D008969:Molecular Sequence Data; D020933:Neurotrophin 3 / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000293:injuries*
D020933:Neurotrophin 3