[Effect of of distamycin A on histone H1 methylation, extraction and formation of UV-inducible crosslinks with DNA in the interphase rat liver nucleus].
Incubation in vitro of rat liver nuclei in the presence of S-adenosyl[methyl-(3)H]methionine ([(3)H] SAM) leads to incorporation of the radioactive label not only into core-histones H3 and H4, but also into linker histone H1. Addition of distamycine A to the incubation medium stimulates label incorporation into histone H1 ~ in 6 times and into histone H3 ~ in 2 times. The presence of distamycine facilitates histone H1 extraction by polyglutamic acid (poly(Glu)) and decreases of UV-induced DNA-histone cross-links formation. These effects give evidence of weakening of H1-chromatin interaction by distamycin to be results of histone H1 position change relative to nucleosome and(or) disturbance of histones H1-H3 interactions so as these histones are exposed to additional methylation.
G I
GI
Kolomiĭtseva
A N
AN
Prusov
T A
TA
Smirnova
B F
BF
Vaniushin
D000818:Animals; D002467:Cell Nucleus / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects / Q000648:ultrastructure; D002843:Chromatin / Q000378:metabolism; D004247:DNA / Q000378:metabolism*; D004214:Distamycins / Q000494:pharmacology*; D006657:Histones / Q000378:metabolism*; D066298:In Vitro Techniques; D007399:Interphase / Q000187:drug effects* / Q000528:radiation effects; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects / Q000648:ultrastructure; D008745:Methylation; D008854:Microscopy, Electron; D011099:Polyglutamic Acid / Q000494:pharmacology; D051381:Rats; D014466:Ultraviolet Rays
D002843:Chromatin; D004214:Distamycins; D006657:Histones; D011099:Polyglutamic Acid; C030000:stallimycin; D004247:DNA