A genome-wide scan for tying-up syndrome in Japanese Thoroughbreds.
Tying-up syndrome, also known as recurrent exertional rhabdomyolysis in Thoroughbreds, is a common muscle disorder for racehorses. In this study, we performed a multipoint linkage analysis using LOKI based on the Bayesian Markov chain Monte Carlo method using 5 half-sib families (51 affected and 277 nonaffected horses in total), and a genome-wide association study (GWAS) using microsatellites (144 affected and 144 nonaffected horses) to map candidate regions for tying-up syndrome in Japanese Thoroughbreds. The linkage analysis identified one strong L-score (82.45) between the loci UCDEQ411 and COR058 (24.9-27.9 Mb) on ECA12. The GWAS identified two suggestive genomic regions on ECA12 (24.9-27.8 Mb) and ECA20 (29.3-33.5 Mb). Based on both results, the genomic region between UCDEQ411 and TKY499 (24.9-27.8 Mb) on ECA12 was the most significant and was considered as a candidate region for tying-up syndrome in Japanese Thoroughbreds.
Department of Molecular Genetics, Laboratory of Racing Chemistry, Utsunomiya, Tochigi, Japan. ttozaki@lrc.or.jp
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T
Tozaki
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Hirota
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S
Sugita
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N
Ishida
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Miyake
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Oki
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Hasegawa
D000818:Animals; D033481:Chromosomes, Mammalian; D055106:Genome-Wide Association Study; D006734:Horse Diseases / Q000235:genetics*; D006736:Horses; D008390:Markov Chains; D018895:Microsatellite Repeats; D009010:Monte Carlo Method; D010375:Pedigree; D012206:Rhabdomyolysis / Q000235:genetics / Q000662:veterinary*