Mechanism of 1-deamino-arginine vasotocin induced natriuresis in rats.
1-Deamino-arginine vasotocin (1dAVT) induced diuresis and a considerable increase in urinary sodium excretion in female Wistar rats. Sodium fractional excretion rose up to 19.3 ± 1.1%. An increase in urine flow rate after 1dAVT (0.5 nmol/kg body-weight [bw]) injection was accompanied by a significant rise of the solute-free water reabsorption. The 1dAVT-induced natriuresis was as high as natriuresis produced by injection of a maximal dose of furosemide (10mg/kg bw). V(1)-receptor antagonists (ОРС-21268, [β-mercapto-β,β-cyclopentamethylenepropionyl(1),O-Me-Tyr(2),Arg(8)]-vasopressin) blocked the increase in urinary sodium excretion after the 1dAVT injection. The 1dAVT-induced natriuresis was strongly correlated with an increase in the urinary cGMP and prostaglandin E(2) excretion. The natriuretic effect of 1dAVT did not depend on the formation of nitric oxide (NO) or atrial natriuretic peptide of which concentration in the rat blood serum remained stable. The above results indicate that the 1dAVT has unique effects on rat kidney compared to all other known diuretics - it induces extremely high natriuresis and stimulates solute-free water reabsorption. Mechanism of the natriuretic effect of 1dAVT includes decrease in tubular sodium reabsorption due to activation of V(1)-like receptors and formation of cGMP and PGЕ(2).
Sechenov Institute of Evolutionary Physiology and Biochemistry of the Russian Academy of Sciences, Thorez Av 44, St Petersburg 194223, Russia.
Tatjana A
TA
Karavashkina
Anna V
AV
Kutina
Elena I
EI
Shakhmatova
Yuri V
YV
Natochin
D000818:Animals; D065092:Antidiuretic Hormone Receptor Antagonists; D009320:Atrial Natriuretic Factor / Q000494:pharmacology; D004231:Diuresis / Q000187:drug effects; D005260:Female; D005665:Furosemide / Q000494:pharmacology; D009318:Natriuresis / Q000187:drug effects; D009569:Nitric Oxide / Q000494:pharmacology; D010880:Piperidines; D015363:Quinolones; D051381:Rats; D017208:Rats, Wistar; D014668:Vasotocin / Q000031:analogs & derivatives* / Q000494:pharmacology
D065092:Antidiuretic Hormone Receptor Antagonists; D010880:Piperidines; D015363:Quinolones; C523848:vasotocin, 1-deamino-arginine-; C068644:OPC 21268; D009569:Nitric Oxide; D005665:Furosemide; D009320:Atrial Natriuretic Factor; D014668:Vasotocin