Quantitative proteomic analysis of cell wall and plasma membrane fractions from multidrug-resistant Acinetobacter baumannii.
Acinetobacter baumannii is a Gram-negative, nonmotile aerobic bacterium that has emerged as an important nosocomial pathogen. Multidrug-resistant (MDR) A. baumannii is difficult to treat with antibiotics, and treatment failure in infected patients is of great concern in clinical settings. To investigate proteome regulation in A. baumannii under antibiotic stress conditions, quantitative membrane proteomic analyses of a clinical MDR A. baumannii strain cultured in subminimal inhibitory concentrations of tetracycline and imipenem were performed using a combination of label-free (one-dimensional electrophoresis-liquid chromatography-tandem mass spectrometry) and label (isobaric tag for relative and absolute quantitation) approaches. In total, 484 proteins were identified, and 302 were classified as outer membrane, periplasmic, or plasma membrane proteins. The clinical A. baumannii strain DU202 responded specifically and induced different cell wall and membrane protein sets that provided resistance to the antibiotics. The induction of resistance-nodulation-cell division transporters and protein kinases, and the repression of outer membrane proteins were common responses in the presence of tetracycline and imipenem. Induction of a tetracycline resistant pump, ribosomal proteins, and iron-uptake transporters appeared to be dependent on tetracycline conditions, whereas β-lactamase and penicillin-binding proteins appeared to be dependent on imipenem conditions. These results suggest that combined liquid chromatography-based proteomic approaches can be used to identify cell wall and membrane proteins involved in the antibiotic resistance of A. baumannii.
Division of Life Science, Korea Basic Science Institute, Daejeon, Korea.
Sung-Ho
SH
Yun
Chi-Won
CW
Choi
Sang-Oh
SO
Kwon
Gun Wook
GW
Park
Kun
K
Cho
Kyung-Hoon
KH
Kwon
Jin Young
JY
Kim
Jong Shin
JS
Yoo
Je Chul
JC
Lee
Jong-Soon
JS
Choi
Soohyun
S
Kim
Seung Il
SI
Kim
D040981:Acinetobacter baumannii / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism*; D002462:Cell Membrane / Q000737:chemistry; D002473:Cell Wall / Q000737:chemistry; D002853:Chromatography, Liquid; D024901:Drug Resistance, Multiple, Bacterial; D015180:Electrophoresis, Gel, Two-Dimensional; D015378:Imipenem / Q000494:pharmacology; D007553:Isotope Labeling; D026901:Membrane Transport Proteins / Q000378:metabolism; D020543:Proteome / Q000032:analysis* / Q000378:metabolism; D040901:Proteomics; D053719:Tandem Mass Spectrometry; D013752:Tetracycline / Q000494:pharmacology; D013753:Tetracycline Resistance
D000900:Anti-Bacterial Agents; D001425:Bacterial Outer Membrane Proteins; D026901:Membrane Transport Proteins; D020543:Proteome; D015378:Imipenem; D013752:Tetracycline