Use of experimental design for the optimization of the production of new secondary metabolites by two Penicillium species.
A fractional factorial design approach has been used to enhance secondary metabolite production by two Penicillium strains. The method was initially used to improve the production of bioactive extracts as a whole and subsequently to optimize the production of particular bioactive metabolites. Enhancements of over 500% in secondary metabolite production were observed for both P. oxalicum and P. citrinum. Two new alkaloids, citrinalins A (5) and B (6), were isolated and identified from P. citrinum cultures optimized for production of minor metabolites.
Instituto de Química de São Carlos, Universidade de São Paulo, CP 780, CEP 13560-970, São Carlos, SP, Brazil.
Eli F
EF
Pimenta
Aline M
AM
Vita-Marques
Aristeu
A
Tininis
Mirna H R
MH
Seleghim
Lara D
LD
Sette
Katyuscya
K
Veloso
Antonio G
AG
Ferreira
David E
DE
Williams
Brian O
BO
Patrick
Doralyn S
DS
Dalisay
Raymond J
RJ
Andersen
Roberto G S
RG
Berlinck
D000470:Alkaloids / Q000737:chemistry / Q000302:isolation & purification*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D002176:Candida albicans / Q000187:drug effects; D046068:Caulerpa / Q000382:microbiology; D002851:Chromatography, High Pressure Liquid; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000187:drug effects; D005260:Female; D006801:Humans; D008386:Marine Biology; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects; D051379:Mice; D008826:Microbial Sensitivity Tests; D008968:Molecular Conformation; D015394:Molecular Structure; D009169:Mycobacterium tuberculosis / Q000187:drug effects; D019906:Nuclear Magnetic Resonance, Biomolecular; D010407:Penicillium / Q000737:chemistry / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D012107:Research Design
D000470:Alkaloids; D000900:Anti-Bacterial Agents; C554220:citrinalin A; C554221:citrinalin B