Insight into the salivary transcriptome and proteome of Dipetalogaster maxima.
Dipetalogaster maxima is a blood-sucking Hemiptera that inhabits sylvatic areas in Mexico. It usually takes its blood meal from lizards, but following human population growth, it invaded suburban areas, feeding also on humans and domestic animals. Hematophagous insect salivary glands produce potent pharmacologic compounds that counteract host hemostasis, including anticlotting, antiplatelet, and vasodilatory molecules. To obtain further insight into the salivary biochemical and pharmacologic complexity of this insect, a cDNA library from its salivary glands was randomly sequenced. Salivary proteins were also submitted to one- and two-dimensional gel electrophoresis (1DE and 2DE) followed by mass spectrometry analysis. We present the analysis of a set of 2728 cDNA sequences, 1375 of which coded for proteins of a putative secretory nature. The saliva 2DE proteome displayed approximately 150 spots. The mass spectrometry analysis revealed mainly lipocalins, pallidipins, antigen 5-like proteins, and apyrases. The redundancy of sequence identification of saliva-secreted proteins suggests that proteins are present in multiple isoforms or derive from gene duplications.
Vector Biology Section, Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, Maryland 20852, USA.
Teresa C F
TC
Assumpção
Sébastien
S
Charneau
Paula B M
PB
Santiago
Ivo M B
IM
Francischetti
Zhaojing
Z
Meng
Carla N
CN
Araújo
Van M
VM
Pham
Rayner M L
RM
Queiroz
Cleudson Nery
CN
de Castro
Carlos André
CA
Ricart
Jaime M
JM
Santana
José M C
JM
Ribeiro
D000595:Amino Acid Sequence; D000818:Animals; D016000:Cluster Analysis; D004591:Electrophoresis, Polyacrylamide Gel; D020869:Gene Expression Profiling; D015723:Gene Library; D019476:Insect Proteins / Q000032:analysis* / Q000145:classification / Q000378:metabolism; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D020543:Proteome / Q000032:analysis* / Q000378:metabolism; D012333:RNA, Messenger / Q000737:chemistry / Q000302:isolation & purification; D012469:Salivary Glands / Q000737:chemistry / Q000378:metabolism; D016415:Sequence Alignment; D014225:Triatominae / Q000378:metabolism*
D019476:Insect Proteins; D020543:Proteome; D012333:RNA, Messenger