Vitamin E supplementation in semen-freezing medium improves the motility and protects sperm from freeze-thaw-induced DNA damage.
This study evaluated the effect of vitamin E supplementation to cryoprotective media on post-thaw motility and DNA integrity of normozoospermic and asthenozoospermic semen samples. The results of this study indicate that supplementation of vitamin E (5 mM) significantly improves the post-thaw motility and DNA integrity in normozoospermic and asthenozoospermic semen samples.
Division of Reproductive Medicine, Kasturba Medical College, Manipal University, Manipal, India. guru.kalthur@manipal.edu
Guruprasad
G
Kalthur
Sujith
S
Raj
Aparna
A
Thiyagarajan
Sangeetha
S
Kumar
Pratap
P
Kumar
Satish Kumar
SK
Adiga
D000328:Adult; D000975:Antioxidants / Q000494:pharmacology; D053627:Asthenozoospermia / Q000628:therapy*; D015925:Cryopreservation; D003451:Cryoprotective Agents / Q000494:pharmacology; D003470:Culture Media / Q000494:pharmacology; D004249:DNA Damage; D005615:Freezing; D006801:Humans; D008297:Male; D027724:Reproductive Techniques, Assisted; D012661:Semen / Q000166:cytology; D013081:Sperm Motility / Q000187:drug effects*; D013094:Spermatozoa / Q000187:drug effects* / Q000502:physiology; D014810:Vitamin E / Q000494:pharmacology*
D000975:Antioxidants; D003451:Cryoprotective Agents; D003470:Culture Media; D014810:Vitamin E