Isolation and characterization of cytotoxic compounds from Euphorbia cornigera Boiss.
Methanolic extract of Euphorbia cornigera shoots was separated using HPLC, affording compounds 1-4. Their structures and relative stereochemistry were established after obtaining their spectroscopic (IR, (1)H, (13)C NMR COSY-45°, HOHAHA, HSQC, HMBC, NOESY, and mass measurement) data. On the basis of these data, the compounds were characterized as 3-O-(2,3-dimethylbutanoyl)-13-O-dodecanoyl-20-O-tetradecanoylingenol (1), 3-O-decanoyl-20-O-hexanoylingenol (2), 3-O-(2,3-dimethylbutanoyl)-13-O-dodecanoyl-20-O-hexadecanoylingenol (3), and 13-O-dodecanoyl-20-O-hexanoylingenol (4); among these compounds, two (1 and 2) were new metabolites while the rest (3 and 4) were known. The MTT cytotoxicity assay was carried out using amrubicin hydrochloride as a positive control. Compound 1 displayed IC(50) as 5.0 and 2.9 μM against RAW and HT-29 cell lines, respectively, which is 5- and 1.5-folds stronger than the control with IC(50) values of 25 and 4.36 μM, respectively.
Department of Chemistry, Gomal University, Dera Ismail Khan, Pakistan.
Imam Bakhsh
IB
Baloch
Musa Kaleem
MK
Baloch
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D004354:Drug Screening Assays, Antitumor; D028481:Euphorbia / Q000737:chemistry*; D019073:HT29 Cells; D006801:Humans; D020128:Inhibitory Concentration 50; D008264:Macrophages / Q000187:drug effects; D051379:Mice; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D010154:Pakistan; D018547:Plant Stems / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*
D000972:Antineoplastic Agents, Phytogenic; D004224:Diterpenes; C008125:ingenol