Counting ions around DNA with anomalous small-angle X-ray scattering.
The majority of charge-compensating ions around nucleic acids form a diffuse counterion "cloud" that is not amenable to investigation by traditional methods that rely on rigid structural interactions. Although various techniques have been employed to characterize the ion atmosphere around nucleic acids, only anomalous small-angle X-ray scattering (ASAXS) provides information about the spatial distribution of ions. Here we present an experimentally straightforward extension of ASAXS that can be used to count the number of ions around nucleic acids.
School of Applied and Engineering Physics, Cornell University, Ithaca, New York 14853, United States.
Suzette A
SA
Pabit
Steve P
SP
Meisburger
Li
L
Li
Joshua M
JM
Blose
Christopher D
CD
Jones
Lois
L
Pollack
D004247:DNA / Q000737:chemistry*; D007477:Ions; D053838:Scattering, Small Angle; D014965:X-Rays
D007477:Ions; D004247:DNA