[Determination of vaccarin in Vaccariae Semen by HPLC].
To establish a HPLC method for the determination of vaccarin in Vaccariae Semen. Analysis was carried out on an Alltima-C18 column (4.6 mm x 250 mm, 5 microm) eluted with methanol -0.3% phosphoric acid as mobile phase in gradient elution. The flow rate was 1.0 mL x min(-1) and detected wavelength was set at 280 nm. The peak areas and injection ammounts of vaccarin showed a good linear relationship in the range of 0.102-1.539 microg, R2 = 0.9997. The average recovery was 100.4%, RSD was 0.81%. The results of the assay of 10 samples showed that the contents of vaccarin varied in the range of 0.46%-0.57%. The method is simple, accurate, reproducible and specific. It can be used for the quality control of Vaccariae Semen.
National Engineering Research Center for Research and Development of Traditional Chinese Medicine Multi-ingredient Drugs, Beijing 100075, China.
He
H
Meng
Yuping
Y
Chen
Wenjie
W
Qin
Xiaojing
X
Tang
Zuguang
Z
Ye
D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D015203:Reproducibility of Results; D031245:Vaccaria / Q000737:chemistry*
D004365:Drugs, Chinese Herbal