New anti-inflammatory and anti-proliferative constituents from fermented red mold rice Monascus purpureus NTU 568.
Six azaphilonoid derivatives, including two new blue fluorescent monapurfluores A (1) and B (2), two known pyridine-containing molecules, monascopyridines C (3) and D (4), and two known monasfluores A (5) and B (6), were isolated and characterized from red mold rice fermented by Monascus purpureus NTU 568. Structural elucidation of new isolates was based on nuclear magnetic resonance (1H- NMR, 13C-NMR, COSY, HMQC, and HMBC) and other spectroscopic analyses. Bioactivity evaluation indicated that 1-6 possessed anti-inflammatory activities with dose-dependent relationships for lipopolysaccharide (LPS)-induced nitric oxide production. Furthermore, 1-4 also showed moderate antiproliferative effects against human laryngeal carcinoma (HEp-2) (IC(50) = 14.81~20.06 μg/mL) and human colon adenocarcinoma (WiDr) (IC(50) = 12.89~21.14 μg/mL).
Department of Biochemical Science and Technology, National Taiwan University, Taipei, Taiwan. kuoyh@nricm.edu.tw
Ya-Wen
YW
Hsu
Li-Chuan
LC
Hsu
Chao-Lin
CL
Chang
Yu-Han
YH
Liang
Yao-Haur
YH
Kuo
Tzu-Ming
TM
Pan
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002460:Cell Line; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D005285:Fermentation; D006801:Humans; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D015394:Molecular Structure; D032741:Monascus / Q000737:chemistry*; D009569:Nitric Oxide / Q000378:metabolism; D011725:Pyridines / Q000737:chemistry / Q000302:isolation & purification
D000893:Anti-Inflammatory Agents; D011725:Pyridines; D009569:Nitric Oxide