A "turn-on" electrochemiluminescent biosensor for detecting Hg2+ at femtomole level based on the intercalation of Ru(phen)3(2+) into ds-DNA.
A well-designed oligonucleotide functionalized for Hg(2+) identification and Ru(phen)(3)(2+) intercalation is used to develop a "turn-on" electrochemiluminescent (ECL) biosensor for the determination of Hg(2+) in a drop (10 μL) of sample.
Research Center for Analytical Sciences, College of Chemistry, Nankai University, Tianjin, 300071, P. R. China.
Chun-Xia
CX
Tang
Yue
Y
Zhao
Xi-Wen
XW
He
Xue-Bo
XB
Yin
D015374:Biosensing Techniques / Q000379:methods; D004247:DNA / Q000737:chemistry*; D055664:Electrochemical Techniques / Q000379:methods; D004566:Electrodes; D006046:Gold / Q000737:chemistry; D007364:Intercalating Agents / Q000737:chemistry*; D008163:Luminescent Measurements / Q000379:methods*; D008628:Mercury / Q000032:analysis*; D009942:Organometallic Compounds / Q000737:chemistry*; D010618:Phenanthrolines / Q000737:chemistry*
D007364:Intercalating Agents; D009942:Organometallic Compounds; D010618:Phenanthrolines; C034282:tris-(1,10-phenanthroline)ruthenium; D006046:Gold; D004247:DNA; D008628:Mercury