The alteration of extracellular signal-regulated kinase (ERK) activity of COS7 cells from interphase to mitosis.
Although many reports have suggested that ERK1/2 activity is increased in interphase and inhibited in mitosis, the change of ERK1/2 activity from interphase to mitosis is largely undefined. In this study, we examined the alteration of ERK1/2 activity in COS7 cells in response to nocodazole. Nocodazole&mdash;treated or nocodazole&mdash;untreated COS7 cells were stimulated by EGF. We found that ERK1/2 activity of nocodazole&mdash;treated cells was decreased with prolonged time of cells treated with nocodazole while being increased in nocodazole&mdash;untreated cells. COS7 cells became round after being treated with nocodazole for 4 hr. And from the time point, ERK1/2 activity was gradually and significantly inhibited. Furthermore, we showed that MEK activity was greatly decreased compared with nocodazole&mdash;untreated cells after the cells were treated by nocodazole for 8 hr and then EGF. In addition, the migration of cells was inhibited in response to nocodazole. Taken together, we concluded that ERK1/2 activity was gradually inhibited from interphase to mitosis and the cell migration was affected by nocodazole.
College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, China. shp1974yuan@sina.com
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D000818:Animals; D019556:COS Cells; D002465:Cell Movement; D002522:Chlorocebus aethiops; D004815:Epidermal Growth Factor / Q000494:pharmacology; D007399:Interphase; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism*; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism*; D008938:Mitosis; D015739:Nocodazole / Q000494:pharmacology; D010766:Phosphorylation; D013997:Time Factors
D004815:Epidermal Growth Factor; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; D015739:Nocodazole