[Screening and identification of differential serum proteins related to dermatitis medicamentosa-like of trichloroethylene].
OBJECTIVE
To screen and identify differential serum proteins which might be involved in dermatitis medicamentosa-like of trichloroethylene (DMLT).


METHODS
Three groups of sera were collected from population exposed to trichloroethylene (TCE) (group I), patients suffering from DMLT (group II), and the healed cases (group III). After removing albumin and IgG in the three pools of sera, a comparative proteomic analysis was carried out. The images were analyzed using ImageMaster Platinum 2D 5.0 to screen the differentially expressed proteins. The protein spots were then subjected to matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and tandem mass spectrometry sequencing of tryptic peptides for further identification.


RESULTS
The depletion of albumin and IgG greatly increased the number of protein spots to 300 ± 12.Five differential spots were identified, which were complement component C4b, apolipoprotein A-I, apolipoprotein C-III apolipoprotein C-II and transthyretin. Compared with group I, the expression levels of complement component C4b in group III and apolipoprotein C-II in group II were up-regulated (1.352 88-fold, 1.512 14-fold, respectively); compared with group I, the expression levels of apolipoprotein A-I, apolipoprotein C-III and transthyretin in group II were down-regulated (1.601 17-fold, 1.034 49-fold, 1.313 35-fold, respectively).


CONCLUSIONS
The findings of this study show that most of the identified differential proteins are closely related to immunity and liver dysfunction, which provides some evidence on elucidating the mechanisms and screening of biomarkers of TCE intoxication.
Toxicology Laboratory, Shenzhen Center for Disease Control and Prevention, Shenzhen 518020, China. bio-research@hotmail.com
Jian-jun
JJ
Liu
Xiu-mei
XM
Xing
Hai-yan
HY
Huang
Jian-hui
JH
Yuan
Xin-yun
XY
Xu
Li
L
Zhou
Xi-fei
XF
Yang
Dao-kui
DK
Fang
D000293:Adolescent; D000328:Adult; D016632:Apolipoprotein A-I / Q000302:isolation & purification; D053305:Apolipoprotein C-III / Q000302:isolation & purification; D015415:Biomarkers / Q000032:analysis; D001798:Blood Proteins / Q000737:chemistry / Q000302:isolation & purification*; D009783:Dermatitis, Occupational / Q000097:blood; D003875:Drug Eruptions / Q000097:blood*; D004781:Environmental Exposure; D005260:Female; D006801:Humans; D008297:Male; D020543:Proteome / Q000032:analysis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014241:Trichloroethylene / Q000009:adverse effects*; D055815:Young Adult
D016632:Apolipoprotein A-I; D053305:Apolipoprotein C-III; D015415:Biomarkers; D001798:Blood Proteins; D020543:Proteome; D014241:Trichloroethylene