Effect of biomolecules from human renal matrix of calcium oxalate monohydrate (CaOx) stones on in vitro calcium phosphate crystallization.
OBJECTIVE
Investigate the activity of high and low molecular weight biomolecules present in the matrix of human calcium oxalate (CaOx) stones not only on the initial mineral phase formation of calcium and phosphate (CaP) but also on its growth and demineralization of the preformed mineral phase.


METHODS
Surgically removed renal stones were analyzed by Fourier Transform Infra Red (FTIR) spectroscopy and only CaOx stones were extracted with 0.05M EGTA, 1 mM PMSF and 1% Β-mercaptoethanol. Renal CaOx stone extract was separated into > 10 kDa and < 10 kDa fractions by dialysis. Activity of both the fractions along with whole extract was studied on the three mineral phases of CaP assay system.


RESULTS
It was interesting to observe that both high and low molecular weight biomolecules extracted from human renal matrix of calcium oxalate (CaOx) stones exhibited different roles in the three mineral phases of CaP. Whole extract exhibited inhibitory activity in all the three assay systems; however, mixed (stimulatory and inhibitory) activity was exhibited by the > 10 kDa and < 10 kDa fractions. SDS-PAGE analysis showed bands of 66 kDa, 80 kDa, 42 kDa in whole EGTA extract lane and > 10 kDa fraction lane.


CONCLUSIONS
Both high and low molecular weight biomolecules extracted from human renal matrix of calcium oxalate (CaOx) stones have a significant influence on calcium and phosphate (CaP) crystallization.
Jaypee University of Information Technology, Waknaghat, India.
Priyadarshini
P
Pathak
Shrawan K
SK
Singh
Chanderdeep
C
Tandon
D002129:Calcium Oxalate / Q000737:chemistry* / Q000378:metabolism; D002130:Calcium Phosphates / Q000737:chemistry*; D003460:Crystallization; D004533:Egtazic Acid; D004591:Electrophoresis, Polyacrylamide Gel; D005583:Fourier Analysis; D006801:Humans; D007669:Kidney Calculi / Q000737:chemistry* / Q000378:metabolism
D002130:Calcium Phosphates; D002129:Calcium Oxalate; D004533:Egtazic Acid; C020243:calcium phosphate