Genetic manipulation of Streptomyces species.
This unit includes general protocols for the genetic manipulation of Streptomyces species, including genomic DNA isolation, genomic library preparation, intergeneric conjugation of Streptomyces with E. coli, generation and transformation of Streptomyces protoplasts, electroporation of Streptomyces mycelia, and colony PCR.
College of Pharmacy, University of Kentucky, Lexington, Kentucky, USA.
S Eric
SE
Nybo
Micah D
MD
Shepherd
Mary A
MA
Bosserman
Jürgen
J
Rohr
D003227:Conjugation, Genetic; D004269:DNA, Bacterial / Q000302:isolation & purification; D018274:Electroporation; D004926:Escherichia coli / Q000235:genetics; D015723:Gene Library; D005818:Genetic Engineering / Q000379:methods*; D005827:Genetics, Microbial / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods; D011523:Protoplasts; D013302:Streptomyces / Q000166:cytology / Q000235:genetics*; D014170:Transformation, Genetic
D004269:DNA, Bacterial