High level expression of a truncated β-mannanase from alkaliphilic Bacillus sp. N16-5 in Kluyveromyces cicerisporus.
A truncated alkaline β-mannanase from alkaliphilic Bacillus sp. N16-5 (MAN330) was expressed and secreted in Kluyveromyces cicerisporus. The recombinant engineered strain for MAN330 production was stable during 80 generations, and the maximum yield of MAN330 reached 3,795 U/ml in 15 l fermenter. MAN330 exhibited similar pH optima, temperature optima, and substrate specificities to its full-length protein (MAN493). However, stability of MAN330 was about 7% higher than that of MAN493 from pH 9-11. MAN330 had about 10% higher stability than MAN493 from 60°C to 80°C.
State Key Laboratory of Genetic Engineering, School of Life Sciences, and Institutes of Biomedical Sciences, Fudan University, Shanghai, 200433, China.
Xian
X
Pan
Jungang
J
Zhou
Ai
A
Tian
Keyi
K
Le
Hanying
H
Yuan
Yanfen
Y
Xue
Yanhe
Y
Ma
Hong
H
Lu
D001407:Bacillus / Q000201:enzymology* / Q000235:genetics; D004795:Enzyme Stability; D006863:Hydrogen-Ion Concentration; D007716:Kluyveromyces / Q000235:genetics / Q000378:metabolism*; D013379:Substrate Specificity; D044902:beta-Mannosidase / Q000235:genetics / Q000378:metabolism*
D044902:beta-Mannosidase