A coding RNA segment that enhances the ribosomal recruitment of chicken ccn1 mRNA.
CCN1, a member of the CCN family of proteins, plays important physiological or pathological roles in a variety of tissues. In the present study, we initially found a highly guanine-cytosine (GC)-rich region of approximately 200 bp near the 5'-end of the open reading frame, which was always truncated by amplification of the corresponding cDNA region through the conventional polymerase chain reaction. An RNA in vitro folding assay and selective ribonuclease digestion of the corresponding segment of the ccn1 mRNA confirmed the involvement of a stable secondary structure. Subsequent RNA electromobility-shift assays demonstrated the specific binding of some cytoplasmic factor(s) in chicken embryo fibroblasts to the RNA segment. Moreover, the corresponding cDNA fragment strongly enhanced the expression of the reporter gene in cis at the 5'-end, but did not do so at the 3'-end. According to the results of a ribosomal assembly test, the effect of the mRNA segment can predominantly be ascribed to the enhancement of transport and/or entry of the mRNA into the ribosome. Finally, the minimal GC-rich mRNA segment that was predicted and demonstrated to form a secondary structure was confirmed to be a functional regulatory element. Thus, we here uncover a novel dual-functionality of the mRNA segment in the ccn1 open reading frame, which segment acts as a cis-element that mediates posttranscriptional gene regulation, while retaining the information for the amino acid sequence of the resultant protein.
Biodental Research Center, Okayama University Dental School, Okayama, Japan.
Yoshiki
Y
Mukudai
Satoshi
S
Kubota
Takanori
T
Eguchi
Kumi
K
Sumiyoshi
Danilo
D
Janune
Seiji
S
Kondo
Satoru
S
Shintani
Masaharu
M
Takigawa
D000818:Animals; D015153:Blotting, Western; D002642:Chick Embryo; D002645:Chickens; D055515:Cysteine-Rich Protein 61 / Q000235:genetics*; D024202:Electrophoretic Mobility Shift Assay; D012313:RNA / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012270:Ribosomes / Q000378:metabolism*
D055515:Cysteine-Rich Protein 61; D012333:RNA, Messenger; D012313:RNA