Effect of acetaminophen (paracetamol) on human osteosarcoma cell line MG63.
OBJECTIVE
To examine the effects of acetaminophen (paracetamol), a nonsteroidal anti-inflammatory drug (NSAID), on different cellular and functional parameters of the human osteosarcoma cell line MG63.


METHODS
Flow cytometry was used to study proliferation, antigenic profile, and phagocytic activity, and radioimmunoassay was used to determine osteocalcin synthesis as a cell differentiation marker.


RESULTS
Short-term treatment with therapeutic doses of paracetamol(5 or 25 μmol/L) reduced cell proliferation, osteocalcin synthesis, and phagocyte activity, and increased the expression of antigens involved in antigen presentation to T lymphocytes (CD80, CD86, HLA-DR).


CONCLUSIONS
These findings suggest that paracetamol activates the osteoblast, inducing its immunogenic action to the detriment of its bone formation capacity.
Department of Nursing, University of Granada, Spain. cldiaz@ugr.es
Lourdes
L
Díaz-Rodríguez
Olga
O
García-Martínez
Manuel
M
Arroyo-Morales
Belén
B
Rubio-Ruiz
Concepción
C
Ruiz
D000082:Acetaminophen / Q000494:pharmacology*; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D017951:Antigen Presentation / Q000187:drug effects; D018122:B7-1 Antigen / Q000096:biosynthesis; D051940:B7-2 Antigen / Q000096:biosynthesis; D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D006684:HLA-DR Antigens / Q000096:biosynthesis; D006801:Humans; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects* / Q000276:immunology / Q000502:physiology; D015675:Osteocalcin / Q000096:biosynthesis; D010587:Phagocytosis / Q000187:drug effects / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology
D000894:Anti-Inflammatory Agents, Non-Steroidal; D018122:B7-1 Antigen; D051940:B7-2 Antigen; C497964:CD86 protein, human; D006684:HLA-DR Antigens; D015675:Osteocalcin; D000082:Acetaminophen