A New Approach for Measuring Single-Cell Oxygen Consumption Rates.
A novel system that has enabled the measurement of single-cell oxygen consumption rates is presented. The experimental apparatus includes a temperature controlled environmental chamber, an array of microwells etched in glass, and a lid actuator used to seal cells in the microwells. Each microwell contains an oxygen sensitive platinum phosphor sensor used to monitor the cellular metabolic rates. Custom automation software controls the digital image data collection for oxygen sensor measurements, which are analyzed using an image-processing program to yield the oxygen concentration within each microwell versus time. Two proof-of-concept experiments produced oxygen consumption rate measurements for A549 human epithelial lung cancer cells of 5.39 and 5.27 fmol/min/cell, closely matching published oxygen consumption rates for bulk A549 populations.
Department of Electrical Engineering, University of Washington, Seattle, WA 98195-2500 USA ( tmolter@washington.edu ; smcquaid@washington.edu ).
Timothy W
TW
Molter
Sarah C
SC
McQuaide
Mark R
MR
Holl
Deirdre R
DR
Meldrum
Joseph M
JM
Dragavon
Judith B
JB
Anderson
A Cody
AC
Young
Lloyd W
LW
Burgess
Mary E
ME
Lidstrom