Identification and characterization of gadolinium(III) complexes in biological tissue extracts.
The gadolinium species present in a rat kidney following intravenous administration of a gadolinium-based magnetic resonance contrast agent (Optimark™, Gadoversetamide injection) to a rat was examined in the present study. The major gadolinium species in the supernatant of the rat kidney tissue extracts was determined by reversed-phase liquid chromatography with online inductively coupled plasma optical emission spectrometry (HPLC-ICP-OES). The identity of the compound was established by liquid chromatography electrospray ionization mass spectrometry (LC-ESI-MS) detection. The principal gadolinium(III) complex in a rat kidney tissue extract was identified as Gd-DTPA-BMEA 24 Hrs and 7 days after a single intravenous injection of Optimark™ (gadoversetamide; Gd-DTPA-BMEA) at a dose of 5 mmol Gd/kg body weight. The study demonstrated for the first time the feasibility of the use of two complementary techniques, HPLC-ICP-OES and HPLC-ESI-MS to study the in vivo behavior of gadolinium-based magnetic resonance contrast media.
Covidien, Imaging Solutions, 675 McDonnell Blvd., Hazelwood, MO 63042, USA. chethaka.kahakachchi@covidien.com
Chethaka L
CL
Kahakachchi
Dennis A
DA
Moore
D000818:Animals; D002417:Cattle; D056148:Chromatography, Reverse-Phase; D003287:Contrast Media / Q000737:chemistry / Q000378:metabolism*; D005682:Gadolinium / Q000737:chemistry* / Q000378:metabolism*; D007275:Injections, Intravenous; D007668:Kidney / Q000737:chemistry / Q000166:cytology; D008297:Male; D015394:Molecular Structure; D004369:Pentetic Acid / Q000031:analogs & derivatives / Q000737:chemistry; D051381:Rats; D014020:Tissue Extracts / Q000737:chemistry
D003287:Contrast Media; C073849:DTPA bis(methoxyethylamide); D014020:Tissue Extracts; D004369:Pentetic Acid; D005682:Gadolinium