Identification of osteoclasts in culture.
Osteoclasts are bone-resorbing multinucleated cells derived from the monocyte-macrophage lineage. Bone-forming osteoblasts play a role in the formation of osteoclasts. Osteoblasts/stromal cells express two cytokines essential for osteoclastogenesis: receptor activator of nuclear factor κB ligand (RANKL) and macrophage colony-stimulating factor (M-CSF). Using RANKL and M-CSF, osteoclasts can be induced from monocyte-macrophage lineage cells even in the absence of osteoblasts. We describe here methods for the identification of osteoclasts formed in vitro.
Department of Biochemistry, Matsumoto Dental University, Nagano, Japan.
Nobuyuki
N
Udagawa
Teruhito
T
Yamashita
Yasuhiro
Y
Kobayashi
Naoyuki
N
Takahashi
D000135:Acid Phosphatase / Q000378:metabolism; D000199:Actins / Q000378:metabolism; D000818:Animals; D001681:Biological Assay; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D003094:Collagen / Q000494:pharmacology; D003804:Dentin / Q000378:metabolism; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000378:metabolism; D051379:Mice; D010010:Osteoclasts / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology; D019030:Receptors, Vitronectin / Q000378:metabolism; D013194:Staining and Labeling; D000071681:Tartrate-Resistant Acid Phosphatase
D000199:Actins; D007527:Isoenzymes; D019030:Receptors, Vitronectin; D003094:Collagen; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase