Estimated frequency of the canine hyperuricosuria mutation in different dog breeds.
BACKGROUND
Hyperuricosuria is a condition that predisposes dogs to urate urolithiasis. A mutation that causes canine hyperuricosuria was previously identified in 3 unrelated dog breeds. The occurrence of the mutation in additional breeds was not determined.


OBJECTIVE
Identify additional breeds that have the hyperuricosuria mutation and estimate the mutant allele frequency in those breeds.


METHODS
Three thousand five hundred and thirty dogs from 127 different breeds were screened for the hyperuricosuria mutation.


METHODS
DNA samples were genotyped by pyrosequencing and allele-specific polymerase chain reaction methods.


RESULTS
Mutant allele frequencies that range from 0.001 to 0.15 were identified in the American Staffordshire Terrier, Australian Shepherd, German Shepherd Dog, Giant Schnauzer, Parson (Jack) Russell Terrier, Labrador Retriever, Large Munsterlander, Pomeranian, South African Boerboel, and Weimaraner breeds.


CONCLUSIONS
The hyperuricosuria mutation has been identified in several unrelated dog breeds. The mutant allele frequencies vary among breeds and can be used to determine an appropriate breeding plan for each breed. A DNA test is available and may be used by breeders to decrease the mutant allele frequency in breeds that carry the mutation. In addition, veterinarians may use the test as a diagnostic tool to identify the cause of urate urolithiasis.
Department of Population Health and Reproduction, School of Veterinary Medicine, University of California-Davis, Davis, CA 95616, USA.
N
N
Karmi
E A
EA
Brown
S S
SS
Hughes
B
B
McLaughlin
C S
CS
Mellersh
V
V
Biourge
D L
DL
Bannasch
D000818:Animals; D004283:Dog Diseases / Q000235:genetics* / Q000652:urine; D004285:Dogs; D020022:Genetic Predisposition to Disease; D009154:Mutation; D014527:Uric Acid / Q000652:urine*
D014527:Uric Acid