Effects of different cross-linking conditions on the properties of genipin-cross-linked chitosan/collagen scaffolds for cartilage tissue engineering.
A cross-linking reagent is required to improve mechanical strength and degradation properties of biopolymers for tissue engineering. To find the optimal preparative method, we prepared diverse genipin-cross-linked chitosan/collagen scaffolds using different genipin concentrations and various cross-linking temperatures and cross-linking times. The compressive strength increased with the increasing of genipin concentration from 0.1 to 1.0%, but when concentration exceeded 1.0%, the compressive strength decreased. Similarly, the compressive strength increased with the increasing of temperature from 4 to 20°C, but when temperature reached 37°C, the compressive strength decreased. Showing a different trend from the above two factors, the effect of cross-linking time on the compressive strength had a single increasing tendency. The other results also demonstrated that the pore size, degradation rate and swelling ratio changed significantly with different cross-linking conditions. Based on our study, 1.0% genipin concentration, 20°C cross-linking temperature and longer cross-linking time are recommended.
Institute of Orthopaedics and Traumatology, Xijing Hospital, The Fourth Military Medical University, Xi'an, People's Republic of China.
Long
L
Bi
Zheng
Z
Cao
Yunyu
Y
Hu
Yang
Y
Song
Long
L
Yu
Bo
B
Yang
Jihong
J
Mu
Zhaosong
Z
Huang
Yisheng
Y
Han
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D001696:Biomechanical Phenomena; D002356:Cartilage / Q000187:drug effects / Q000378:metabolism; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry*; D003094:Collagen / Q000737:chemistry* / Q000187:drug effects; D019245:Compressive Strength / Q000187:drug effects / Q000502:physiology; D003432:Cross-Linking Reagents / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005260:Female; D057889:Iridoid Glycosides / Q000494:pharmacology*; D039823:Iridoids; D008422:Materials Testing; D016062:Porosity; D011817:Rabbits; D013696:Temperature; D013997:Time Factors; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D057889:Iridoid Glycosides; D039823:Iridoids; D003094:Collagen; D048271:Chitosan; C007834:genipin