Statistical optimization of sulfite pretreatment of corncob residues for high concentration ethanol production.
In this study, a central composite design of response surface method was used to optimize sulfite pretreatment of corncob residues, in respect to sulfite charge (5-10%), treatment time (1-2h), liquid/solid (l/s) ratio (6:1-10:1) and temperature (150-180°C) for maximizing glucose production in enzymatic hydrolysis process. The relative optimum condition was obtained as follows: sulfite charge 7.1%, l/s ratio 7.6:1, temperature 156°C for 1.4h, corresponding to 79.3% total glucan converted to glucose+cellobiose. In the subsequent simultaneous saccharification and fermentation (SSF) experiments using 15% glucan substrates pretreated under this kind of conditions, 60.8 g ethanol l(-1) with 72.2% theoretical yield was obtained.
Institute of Nuclear and New Energy Technology, Tsinghua University, Beijing, PR China.
Ke-Ke
KK
Cheng
Wei
W
Wang
Jian-An
JA
Zhang
Qiang
Q
Zhao
Jin-Ping
JP
Li
Jian-Wei
JW
Xue
D002480:Cellulase / Q000737:chemistry*; D003198:Computer Simulation; D003627:Data Interpretation, Statistical; D000431:Ethanol / Q000138:chemical synthesis* / Q000378:metabolism*; D008954:Models, Biological; D008956:Models, Chemical; D015233:Models, Statistical; D035261:Plant Components, Aerial / Q000737:chemistry; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D013447:Sulfites / Q000737:chemistry*; D003313:Zea mays / Q000737:chemistry* / Q000382:microbiology*
D013447:Sulfites; D000431:Ethanol; D002480:Cellulase