Raman spectroscopy--a new method for the intra-operative assessment of axillary lymph nodes.
Sentinel Lymph Node Biopsy has become the standard surgical procedure for the sampling of axillary lymph nodes in breast cancer. Intra operative node assessment is currently not offered to the majority of patients but would allow definitive axillary surgery to take place immediately. This would confer benefits both to the patient and to the healthcare system. Our experimental study aims to demonstrate that a Raman spectroscopy probe device could overcome many of the disadvantages of current intra-operative analysis techniques. 38 axillary lymph nodes, 25 negative and 13 positive from 20 patients undergoing breast surgery for invasive breast cancer were assessed using a commercially available Raman spectroscopy probe. Spectra were assessed using principal component fed linear discriminant analysis trained by the histopathology results. Leave one node out cross validation achieved a sensitivity of up to 92% and a specificity of up to 100% in differentiating between normal and metastatic lymph nodes.
Department of Biophotonics Research, Gloucestershire Hospitals NHS Foundation Trust, Gloucester Royal Hospital, Leadon House, Gloucester, Glos, UK GL1 3NN. j.horsnell@medical-research.com
Jonathan
J
Horsnell
Paul
P
Stonelake
Jonathan
J
Christie-Brown
Geeta
G
Shetty
Joanne
J
Hutchings
Catherine
C
Kendall
Nicholas
N
Stone
D001365:Axilla / Q000473:pathology / Q000601:surgery*; D001943:Breast Neoplasms / Q000175:diagnosis / Q000473:pathology / Q000601:surgery; D005260:Female; D006801:Humans; D007432:Intraoperative Period; D008197:Lymph Node Excision; D008198:Lymph Nodes / Q000473:pathology* / Q000601:surgery*; D008207:Lymphatic Metastasis / Q000473:pathology; D021701:Sentinel Lymph Node Biopsy / Q000379:methods*; D013059:Spectrum Analysis, Raman / Q000379:methods*