Rapid temperature-dependent antibody ranking using Biacore A100.
The ability to select antibodies with stable thermodynamic profiles can greatly enhance the performance of point-of-care (POC) devices allowing reproducible "on-the-spot" analysis for markers of clinical and environmental relevance. We show that by ranking antibodies based on their kinetic profiles at two different temperatures, greater information content is acquired, facilitating the selection of antibodies with desired binding characteristics. Observed binding patterns highlight the importance of temperature to assay design, which should be fully taken into consideration to ensure that the appropriate antibody is selected for the desired test.
Dublin City University, Ireland.
P
P
Leonard
C J
CJ
Hayes
R
R
O'Kennedy
D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000145:classification / Q000276:immunology; D001665:Binding Sites; D015374:Biosensing Techniques / Q000295:instrumentation; D007700:Kinetics; D017430:Prostate-Specific Antigen / Q000276:immunology; D013696:Temperature; D013816:Thermodynamics
D000911:Antibodies, Monoclonal; D017430:Prostate-Specific Antigen