Enhanced dendritic action potential backpropagation in parvalbumin-positive basket cells during sharp wave activity.
In this study two-photon imaging and single cell electrophysiological measurements were carried out in PV+ hippocampal interneurons to compare the dendritic calcium dynamics of somatically evoked backpropagating action potentials (BAPs) and in vitro sharp wave oscillation (SPW) activated BAPs at different distances from the soma. In the case of 300 μm thick, non-oscillating slices, the BAP-evoked Ca(2+) (BAP-Ca(2+)) influx propagated along the dendritic tree in a non-uniform manner and its amplitude gradually reduced when measured at more distal regions. In contrast to the evoked BAP-Ca(2+)s, the spontaneous SPW-induced Ca(2+) influx had only a small distance-dependent decrement. Our results suggest that similarly to nicotinic acetylcholine receptor activation, synaptic activity during hippocampal SPWs increases AP backpropagation into distant dendritic segments. Bath application of Nimodipine, a specific Ca(2+) channel blocker and tetrodotoxine decreased the amplitude of the somatically evoked Ca(2+) influx, which suggests that L-type Ca(2+) channels play an important role both during somatically evoked and SPW-induced BAPs.
Institute of Experimental Medicine, Hungarian Academy of Sciences, Szigony str 43, 1083 Budapest, Hungary.
Balázs
B
Chiovini
Gergely F
GF
Turi
Gergely
G
Katona
Attila
A
Kaszás
Ferenc
F
Erdélyi
Gábor
G
Szabó
Hannah
H
Monyer
Attila
A
Csákányi
E Sylvester
ES
Vizi
Balázs
B
Rózsa
D000200:Action Potentials; D000818:Animals; D015220:Calcium Channels / Q000502:physiology; D003713:Dendritic Cells / Q000378:metabolism / Q000502:physiology*; D006624:Hippocampus / Q000378:metabolism / Q000502:physiology; D007395:Interneurons / Q000502:physiology; D015640:Ion Channel Gating; D051379:Mice; D008822:Mice, Transgenic; D010100:Oxygen / Q000378:metabolism; D010320:Parvalbumins / Q000378:metabolism*
D015220:Calcium Channels; D010320:Parvalbumins; D010100:Oxygen