Blood arachidonic acid and HDL cholesterol influence the phagocytic abilities of human monocytes/macrophages.
OBJECTIVE
Many immunomodulators may intensify the process of phagocytosis in monocytes. Among them are the fatty acids contained in cellular membrane phospholipids. But in the available literature there are no reports on how blood fatty acids and lipoproteins can modulate the phagocytic abilities of cells. Therefore, the aim of this study was the evaluation of the phagocytic activity of monocytes isolated from the blood of healthy human subjects with defined fatty acids and lipoprotein content.


METHODS
Cells obtained from 24 donors were used for measuring phagocytic activity and for the quantification of serum fatty acids, total cholesterol, TG, HDL, and LDL, respectively. Phagocytosis was determined using a PHAGOTEST kit, fatty acids using a GC chromatograph, and lipids using test kits.


CONCLUSIONS
The analysis shows an influence of serum HDL concentrations on the process of phagocytosis in the isolated cells and suggests that the concentration of arachidonic acid in blood is a factor that determines the phagocytic ability of monocytes. Moreover, the concentration of conjugated linoleic acid trans-10 cis-12 has considerable influence on phagocytosis.
Departments of Biochemistry and Human Nutrition, Pomeranian Medical University, al. Powstancow Wlkp. 72, Szczecin, Poland. izagut@poczta.onet.pl
I
I
Gutowska
M
M
Baśkiewicz
B
B
Machaliński
D
D
Chlubek
E
E
Stachowska
D000328:Adult; D016718:Arachidonic Acid / Q000097:blood* / Q000378:metabolism; D008076:Cholesterol, HDL / Q000097:blood* / Q000378:metabolism; D008078:Cholesterol, LDL / Q000097:blood; D004041:Dietary Fats / Q000097:blood / Q000378:metabolism; D006801:Humans; D044243:Linoleic Acids, Conjugated / Q000097:blood / Q000378:metabolism; D008074:Lipoproteins / Q000097:blood / Q000737:chemistry; D008264:Macrophages / Q000378:metabolism / Q000502:physiology*; D008297:Male; D009000:Monocytes / Q000378:metabolism / Q000502:physiology*; D010587:Phagocytosis; D014280:Triglycerides / Q000097:blood / Q000378:metabolism; D055815:Young Adult
D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D004041:Dietary Fats; D044243:Linoleic Acids, Conjugated; D008074:Lipoproteins; D014280:Triglycerides; C496197:trans-10,cis-12-conjugated linoleic acid; D016718:Arachidonic Acid