One-pot production of 18F-biotin by conjugation with 18F-FDG for pre-targeted imaging: synthesis and radio-labelling of a PEGylated precursor.
The biotin-avidin affinity system is exploited in pre-targeted imaging using avidin-conjugated antibodies. (18)F-FDG is available at all PET centres. (18)F-FDG forms oximes by reaction with oxyamine. Herein we describe the synthesis of oxyamine-funtionalised biotin, its (18)F-labelling by conjugation with (18)F-FDG and confirm its ability to interact with avidin.
Aberdeen Biomedical Imaging Centre, School of Medical Sciences, University of Aberdeen, UK.
Michael
M
Simpson
Laurent
L
Trembleau
Richard W
RW
Cheyne
Tim A D
TA
Smith
D001360:Avidin / Q000737:chemistry; D001710:Biotin / Q000031:analogs & derivatives* / Q000138:chemical synthesis*; D005462:Fluorine Radioisotopes; D019788:Fluorodeoxyglucose F18; D010091:Oximes / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry
D005462:Fluorine Radioisotopes; D010091:Oximes; D019788:Fluorodeoxyglucose F18; D001360:Avidin; D011092:Polyethylene Glycols; D001710:Biotin