Changes in flavonoid content and tyrosinase inhibitory activity in kenaf leaf extract after far-infrared treatment.
The tyrosinase inhibitory activity of ethanolic extract of kenaf (Hibiscus cannabinus L.) leaf was evaluated before and after subjecting it to far-infrared (FIR) irradiation. The main component of the extract was analyzed as kaempferitrin (kaempferol-3,7-O-α-dirhamnoside). Prior to FIR irradiation, no inhibitory activity of the extract was detected in a tyrosinase assay. However, after FIR irradiation for 1h at 60°C, significant tyrosinase inhibitory activity (IC(50)=3500 ppm) was observed in it. In HPLC analysis, derhamnosylation products (kaempferol, afzelin, and α-rhamnoisorobin) were detected. The inhibitory activity may be due to the existence of derhamnosylation products. This study demonstrated that FIR irradiation can be used as a convenient tool for deglycosylation of flavonoid glycoside.
R&D Center, AmorePacific Corporation, Kyounggi-do, Yongin, Republic of Korea. thiocarbon@freechal.com
Ho Sik
HS
Rho
Soo Mi
SM
Ahn
Bum Chun
BC
Lee
Myung Kyoo
MK
Kim
Amal Kumar
AK
Ghimeray
Cheng Wu
CW
Jin
Dong Ha
DH
Cho
D005419:Flavonoids / Q000737:chemistry / Q000378:metabolism*; D031584:Hibiscus / Q000737:chemistry*; D007259:Infrared Rays; D044949:Kaempferols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D008362:Mannosides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D044945:Proanthocyanidins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology
D005419:Flavonoids; D044949:Kaempferols; D008362:Mannosides; D010936:Plant Extracts; D044945:Proanthocyanidins; C477954:afzelin; C006552:kaempferol; D014442:Monophenol Monooxygenase; C042728:lespenefril