An efficient protocol for genetic transformation of Platycodon grandiflorum with Agrobacterium rhizogenes.
The balloon flower (Platycodon grandiflorum) is a popular traditional medicinal plant used in Korea to treat conditions such as bronchitis, asthma, tuberculosis, diabetes, and inflammatory diseases. Recently, immunopharmacological research identified triterpenoid and saponin as important active compounds in P. grandiflorum. To study and extract these compounds and other metabolites from P. grandiflorum, a technique was developed for producing hairy root cultures, which are a reliable source of plant compounds. To achieve this, the activity of Agrobacterium rhizogenes was exploited, which can transfer DNA segments into plant genomes after infecting them. In this study, the A. rhizogenes strain R1000 was determined that had the highest infection frequency (87.5%) and induced the most hairy roots per plant, and the concentration of antibiotics (75 mg/l kanamycin) was elucidated for selection after transformation. Wild-type and transgenic hairy roots contained various phenolic compounds, although both of them had similar concentrations of phenolic compounds. In the future, the protocols described here should be useful for studying and extracting valuable metabolites such as phenolic compounds from P. grandiflorum hairy root cultures.
Department of Crop Science, College of Agriculture and Life Sciences, Chungnam National University, 220 Gung-Dong, Yuseong-Gu, Daejeon, 305-764, Korea.
Nam Il
NI
Park
Pham Anh
PA
Tuan
Xiaohua
X
Li
Yong Kyoung
YK
Kim
Tae Jin
TJ
Yang
Sang Un
SU
Park
D002851:Chromatography, High Pressure Liquid; D017931:DNA Primers / Q000235:genetics; D007612:Kanamycin; D018517:Plant Roots / Q000254:growth & development / Q000378:metabolism; D031238:Platycodon / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012231:Rhizobium / Q000235:genetics*; D012503:Saponins / Q000302:isolation & purification; D046509:Tissue Culture Techniques / Q000379:methods*; D014170:Transformation, Genetic / Q000235:genetics*; D014315:Triterpenes / Q000302:isolation & purification
D017931:DNA Primers; D012503:Saponins; D014315:Triterpenes; D007612:Kanamycin