Developmental competence of parthenogenetic mouse and human embryos after chemical or electrical activation.
Parthenogenetic reconstruction is one major strategy to create patient-specific stem cells. The aim of this study was to find the best artificial activation protocol for parthenogenetic activation of mouse and human oocytes comparing different methods. In a first set of experiments, in-vivo matured mouse oocytes and human failed-fertilized, in-vitro and in-vivo matured oocytes were artificially activated by a chemical (ionomycin) or electrical stimulus. In a second set of experiments, a combination of activating agents (electrical pulses followed by ionomycin or SrCl(2)) was applied in an aim to improve developmental competence. All embryos were evaluated daily until day 6 after activation. Mouse blastocysts were differentially stained to evaluate blastocyst quality. For mouse oocytes and human failed-fertilized oocytes, blastocyst development was significantly higher after electrical activation (P<0.05). For human in-vitro and in-vivo matured oocytes, blastocyst formation was only obtained after electrical activation of in-vitro matured oocytes. After combining activating agents, no differences in development could be observed. In conclusion, this study revealed that for both mouse and human oocytes development to the blastocyst stage was significantly better after electrical activation compared with chemical activation. Combining activating agents had no further positive effect on developmental potential.
Department of Reproductive Medicine, Ghent University Hospital, De Pintelaan 185, 9000 Ghent, Belgium. Karen.Versieren@UGent.be
Karen
K
Versieren
Björn
B
Heindryckx
Sylvie
S
Lierman
Jan
J
Gerris
Petra
P
De Sutter
D000818:Animals; D004558:Electric Stimulation / Q000379:methods; D046149:Embryo Culture Techniques; D004622:Embryo, Mammalian / Q000187:drug effects / Q000196:embryology*; D047108:Embryonic Development / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D015759:Ionomycin / Q000494:pharmacology*; D051379:Mice; D009865:Oocytes / Q000187:drug effects* / Q000502:physiology; D010312:Parthenogenesis / Q000187:drug effects* / Q000502:physiology; D057905:Stem Cell Research; D013324:Strontium / Q000494:pharmacology
D015759:Ionomycin; C025700:strontium chloride; D013324:Strontium