The influence of type-I collagen-coated PLLA aligned nanofibers on growth of blood outgrowth endothelial cells.
Nanofibrous scaffolds have been applied widely in tissue engineering to simulate the nanostructure of natural extracellular matrix (ECM) and promote cell bioactivity. The aim of this study was to design a biocompatible nanofibrous scaffold for blood outgrowth endothelial cells (BOECs) and investigate the interaction between the topography of the nanofibrous scaffold and cell growth. Poly(L-lactic acid) (PLLA) random and aligned nanofibers with a uniform diameter distribution were fabricated by electrospinning. NH(3) plasma etching was used to create a hydrophilic surface on the nanofibers to improve type-I collagen adsorption; the conditions of the NH(3) plasma etching were optimized by XPS and water contact angle analysis. Cell attachment, proliferation, viability, phenotype and morphology of BOECs cultured on type-I collagen-coated PLLA film (col-Film), random fibers (col-RFs) and aligned fibers (col-AFs) were detected over a 7 day culture period. The results showed that collagen-coated PLLA nanofibers improved cell attachment and proliferation; col-AFs induced the directional growth of cells along the aligned nanofibers and enhanced endothelialization. We suggest that col-AFs may be a potential implantable scaffold for vascular tissue engineering.
State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, People's Republic of China.
Zhang-Qi
ZQ
Feng
Hui-Jun
HJ
Lu
Michelle K
MK
Leach
Ning-Ping
NP
Huang
Yi-Chun
YC
Wang
Chang-Jian
CJ
Liu
Zhong-Ze
ZZ
Gu
D000818:Animals; D016880:Anisotropy; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis*; D024042:Collagen Type I / Q000737:chemistry* / Q000494:pharmacology*; D042783:Endothelial Cells / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D019344:Lactic Acid / Q000737:chemistry*; D008422:Materials Testing; D008968:Molecular Conformation; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D011091:Polyesters; D011108:Polymers / Q000737:chemistry*; D011817:Rabbits; D023822:Tissue Engineering / Q000295:instrumentation / Q000379:methods; D054457:Tissue Scaffolds
D020099:Coated Materials, Biocompatible; D024042:Collagen Type I; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide)