Dynamic gene expression of GH/PRL-family hormone receptors in gill and kidney during freshwater-acclimation of Mozambique tilapia.
In teleosts, prolactin (PRL) and growth hormone (GH) act at key osmoregulatory tissues to regulate hydromineral balance. This study was aimed at characterizing patterns of expression for genes encoding receptors for the GH/PRL-family of hormones in the gill and kidney of Mozambique tilapia (Oreochromis mossambicus) during freshwater (FW)-acclimation. Transfer of seawater (SW)-acclimated tilapia to FW elicited rapid and sustained increases in plasma levels and pituitary gene expression of PRL177 and PRL188; plasma hormone and pituitary mRNA levels of GH were unchanged. In the gill, PRL receptor 1 (PRLR1) mRNA increased markedly after transfer to FW by 6h, while increases in GH receptor (GHR) mRNA were observed 48 h and 14 d after the transfer. By contrast, neither PRLR2 nor the somatolactin receptor (SLR) was responsive to FW transfer. Paralleling these endocrine responses were marked increases in branchial gene expression of a Na+/Cl- cotransporter and a Na+/H+ exchanger, indicators of FW-type mitochondrion-rich cells (MRCs), at 24 and 48 h after FW transfer, respectively. Expression of Na+/K+/2Cl- cotransporter, an indicator of SW-type MRCs, was sharply down-regulated by 6h after transfer to FW. In kidney, PRLR1, PRLR2 and SLR mRNA levels were unchanged, while GHR mRNA was up-regulated from 6h after FW transfer to all points thereafter. Collectively, these results suggest that the modulation of the gene expression for PRL and GH receptors in osmoregulatory tissues represents an important aspect of FW-acclimation of tilapia.
Hawaii Institute of Marine Biology, University of Hawaii, Kaneohe, HI 96744, USA.
Jason P
JP
Breves
Andre P
AP
Seale
Ryan E
RE
Helms
Christian K
CK
Tipsmark
Tetsuya
T
Hirano
E Gordon
EG
Grau
D000064:Acclimatization; D000818:Animals; D001934:Branchial Region / Q000378:metabolism; D005618:Fresh Water; D005880:Gills / Q000378:metabolism; D013006:Growth Hormone / Q000096:biosynthesis; D007668:Kidney / Q000378:metabolism; D008297:Male; D009928:Organ Specificity; D010902:Pituitary Gland / Q000378:metabolism; D011388:Prolactin / Q000096:biosynthesis; D011981:Receptors, Prolactin / Q000096:biosynthesis*; D011986:Receptors, Somatotropin / Q000096:biosynthesis*; D028021:Sodium-Potassium-Chloride Symporters / Q000096:biosynthesis; D000254:Sodium-Potassium-Exchanging ATPase / Q000096:biosynthesis; D017210:Tilapia / Q000378:metabolism*; D014158:Transcription, Genetic; D014882:Water-Electrolyte Balance
D011981:Receptors, Prolactin; D011986:Receptors, Somatotropin; D028021:Sodium-Potassium-Chloride Symporters; D011388:Prolactin; D013006:Growth Hormone; D000254:Sodium-Potassium-Exchanging ATPase