Inhibition of CYP3A mRNA and protein expression, and enzymatic activity, by enrofloxacin in chickens.
This study was to investigate the effect of enrofloxacin (EF) on CYP3A in chicken by using quantitative reverse transcription-polymerase chain reaction and immunodetected. The treated chickens were given 5, 25 and 125 mg/kg of EF while the control chickens were treated with the same volume saline. There was no significant difference between the low dose group and controls in the concentration of hepatic microsome protein and total CYP content, while the middle and high dose EF caused the down regulation. Depression of the CYP3A activity, mRNA and protein were observed in treated chickens, and the inhibition degree was different from each group. It was concluded that EF caused the inhibition of CYP3A both in genetic transcription and protein levels. But the inhibition metabolism still needs further researches.
Institute of Hygiene and Environmental Medicine, Academy of Military Medical Sciences, Tianjin, China.
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Hu
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Gao
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Cheng
D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D015153:Blotting, Western / Q000662:veterinary; D002645:Chickens / Q000378:metabolism; D051544:Cytochrome P-450 CYP3A / Q000032:analysis / Q000096:biosynthesis*; D065692:Cytochrome P-450 CYP3A Inhibitors; D000077422:Enrofloxacin; D024841:Fluoroquinolones / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects; D008297:Male; D008862:Microsomes, Liver / Q000737:chemistry / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D014176:Protein Biosynthesis / Q000187:drug effects*; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary
D000890:Anti-Infective Agents; D065692:Cytochrome P-450 CYP3A Inhibitors; D024841:Fluoroquinolones; D012333:RNA, Messenger; D000077422:Enrofloxacin; D051544:Cytochrome P-450 CYP3A