Preharvest internalization of Escherichia coli O157:H7 into lettuce leaves, as affected by insect and physical damage.
Environmental pests may serve as reservoirs and vectors of zoonotic pathogens to leafy greens; however, it is unknown whether insect pests feeding on plant tissues could redistribute these pathogens present on the surface of leaves to internal sites. This study sought to differentiate the degree of tissue internalization of Escherichia coli O157:H7 when applied at different populations on the surface of lettuce and spinach leaves, and to ascertain whether lettuce-infesting insects or physical injury could influence the fate of either surface or internalized populations of this enteric pathogen. No internalization of E. coli O157:H7 occurred when lettuce leaves were inoculated with 4.4 log CFU per leaf, but it did occur when inoculated with 6.4 log CFU per leaf. Internalization was statistically greater when spinach leaves were inoculated on the abaxial (underside) than when inoculated on the adaxial (topside) side, and when the enteric pathogen was spread after surface inoculation. Brief exposure (∼18 h) of lettuce leaves to insects (5 cabbage loopers, 10 thrips, or 10 aphids) prior to inoculation with E. coli O157:H7 resulted in significantly reduced internalized populations of the pathogen within these leaves after approximately 2 weeks, as compared with leaves not exposed to insects. Surface-contaminated leaves physically injured through file abrasions also had significantly reduced populations of both total and internalized E. coli O157:H7 as compared with nonabraded leaves 2 weeks after pathogen exposure.
Center for Food Safety, 1109 Experiment Street, Department of Crop and Soil Sciences, University of Georgia, Griffin, Georgia 30223, USA. mericks@uga.edu
Marilyn C
MC
Erickson
Jean
J
Liao
Alison S
AS
Payton
David G
DG
Riley
Cathy C
CC
Webb
Lindsey E
LE
Davey
Sophia
S
Kimbrel
Li
L
Ma
Guodong
G
Zhang
Ian
I
Flitcroft
Michael P
MP
Doyle
Larry R
LR
Beuchat
D000818:Animals; D001422:Bacterial Adhesion / Q000502:physiology*; D015169:Colony Count, Microbial; D003257:Consumer Product Safety; D019453:Escherichia coli O157 / Q000254:growth & development / Q000502:physiology*; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology; D006801:Humans; D007313:Insecta; D018545:Lactuca / Q000382:microbiology*; D018515:Plant Leaves / Q000382:microbiology; D018724:Spinacia oleracea / Q000382:microbiology