Structural studies of Hantaan virus.
Hantaan virus is the prototypic member of the Hantavirus genus within the family Bunyaviridae and is a causative agent of the potentially fatal hemorrhagic fever with renal syndrome. The Bunyaviridae are a family of negative-sense RNA viruses with three-part segmented genomes. Virions are enveloped and decorated with spikes derived from a pair of glycoproteins (Gn and Gc). Here, we present cryo-electron tomography and single-particle cryo-electron microscopy studies of Hantaan virus virions. We have determined the structure of the tetrameric Gn-Gc spike complex to a resolution of 2.5 nm and show that spikes are ordered in lattices on the virion surface. Large cytoplasmic extensions associated with each Gn-Gc spike also form a lattice on the inner surface of the viral membrane. Rod-shaped ribonucleoprotein complexes are arranged into nearly parallel pairs and triplets within virions. Our results differ from the T=12 icosahedral organization found for some bunyaviruses. However, a comparison of our results with the previous tomographic studies of the nonpathogenic Tula hantavirus indicates a common structural organization for hantaviruses.
Department of Biological Sciences, Purdue University, 915 W. State Street, West Lafayette, IN 47907-2054, USA.
Anthony J
AJ
Battisti
Yong-Kyu
YK
Chu
Paul R
PR
Chipman
Bärbel
B
Kaufmann
Colleen B
CB
Jonsson
Michael G
MG
Rossmann
D000818:Animals; D002522:Chlorocebus aethiops; D020285:Cryoelectron Microscopy; D055032:Electron Microscope Tomography; D018063:Hantaan virus / Q000648:ultrastructure*; D046911:Macromolecular Substances / Q000648:ultrastructure; D014709:Vero Cells; D014764:Viral Proteins / Q000648:ultrastructure; D014771:Virion / Q000648:ultrastructure*
D046911:Macromolecular Substances; D014764:Viral Proteins