New species genetic approach to identify strains of mitis group streptococci that are donors of rifampin resistance to Streptococcus pneumoniae.
Eight rifampin-resistant streptococci of the mitis group were identified at the species level by using a concatenated 16S rRNA gene-sodA-rpoB-hlpA sequence. Characterization of their rpoB alleles showed single amino acid changes involved in rifampin resistance. Comparison of RpoB sequences from pneumococcal recombinant isolates, viridans isolates, and type strains revealed a species-specific amino acid signature, which allowed it to be ascertained that recombinant RpoBs were originated in genetic interchanges with Streptococcus mitis and Streptococcus oralis.
Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain. mjferrandiz@isciii.es
María-José
MJ
Ferrándiz
Carmen
C
Ardanuy
Josefina
J
Liñares
Luz
L
Balsalobre
María Teresa
MT
García
Adela G
AG
de la Campa
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012293:Rifampin / Q000494:pharmacology*; D034361:Streptococcus mitis / Q000187:drug effects / Q000235:genetics; D019533:Streptococcus oralis / Q000187:drug effects / Q000235:genetics; D013296:Streptococcus pneumoniae / Q000145:classification / Q000187:drug effects* / Q000235:genetics*
D000900:Anti-Bacterial Agents; D012336:RNA, Ribosomal, 16S; D012293:Rifampin