Characterisation of antibodies to bovine Toll-like receptor (TLR)-2 and cross-reactivity with ovine TLR2.
Host recognition of conserved pathogen-associated molecular patterns (PAMPs) and their interactions with pattern-recognition receptors, including the Toll-like receptors (TLR) is essential for innate immune response induction. The TLR1 family (TLR1, 2, 6 and 10) is involved in the recognition of gram-positive and gram-negative bacteria and heterodimers of TLR1 or TLR6 with TLR2 are crucial for the identification of several PAMPs. Studies on cell surface expression of TLR in ruminants are hampered by the lack of specific antibodies and no convincingly cross-reactive anti-human antibodies have been described so far. We describe herein four antibodies which recognise bovine TLR2. Differences in TLR2 expression were evident on bovine antigen presenting cells with high level expression on peripheral blood monocytes and monocyte-derived macrophages. Lower levels of expression were evident on dendritic cell populations derived in vitro and ex vivo, and on alveolar macrophages. One of the antibodies recognised TLR2 expression on ovine peripheral blood monocytes. The identification of antibodies specific for bovine and ovine TLR2 will facilitate studies of the role of this important PRR in the initiation of immune responses to important pathogens.
Institute for Animal Health, High Street, Compton, Newbury, Berkshire RG207NN, United Kingdom.
Lai Shan
LS
Kwong
Richard
R
Parsons
Robert
R
Patterson
Tracey J
TJ
Coffey
Leenadevi
L
Thonur
Jung-Su
JS
Chang
George
G
Russell
David
D
Haig
Dirk
D
Werling
Jayne C
JC
Hope
D000818:Animals; D000906:Antibodies / Q000378:metabolism*; D002417:Cattle / Q000378:metabolism*; D003429:Cross Reactions; D005786:Gene Expression Regulation / Q000502:physiology; D057809:HEK293 Cells; D006801:Humans; D012756:Sheep / Q000378:metabolism*; D051195:Toll-Like Receptor 2 / Q000235:genetics / Q000378:metabolism*
D000906:Antibodies; D051195:Toll-Like Receptor 2