Effect of biosurfactants on laccase production and phenol biodegradation in solid-state fermentation.
The effects of two biosurfactants, tea saponin (TS) and rhamnolipid (RL), on the production of laccase and the degradation of phenol by P. simplicissimum were investigated in solid-state fermentation consisting of rice straw, rice bran, and sawdust. Firstly, the effects of phenol on the fermentation process were studied in the absence of surfactants. Then, a phenol concentration of 3 mg/g in the fermentation was selected for detailed research with the addition of biosurfactants. The results showed that TS and RL at different concentrations had stimulative effects on the enzyme activity of laccase. The highest laccase activities during the fermentation were enhanced by 163.7%, 68.2%, and 23.3% by TS at concentrations of 0.02%, 0.06%, and 0.10%, respectively. As a result of the enhanced laccase activity, the efficiency of phenol degradation was also improved by both biosurfactants. RL caused a significant increase of fungal biomass in the early stage of the fermentation, while TS had an inhibitory effect in the whole process. These results indicated that RL could mitigate the negative effects of phenol on fungal growth and consequently improve laccase production and phenol degradation. TS was potentially applicable to phenol-polluted solid-state fermentation.
College of Environmental Science and Engineering, Hunan University, Changsha, People's Republic of China.
Mei-Fang
MF
Zhou
Xing-Zhong
XZ
Yuan
Hua
H
Zhong
Zhi-Feng
ZF
Liu
Hui
H
Li
Li-Li
LL
Jiang
Guang-Ming
GM
Zeng
D001426:Bacterial Proteins / Q000378:metabolism*; D001673:Biodegradation, Environmental / Q000187:drug effects; D018533:Biomass; D028241:Camellia sinensis / Q000737:chemistry; D005285:Fermentation / Q000187:drug effects; D005656:Fungal Proteins / Q000378:metabolism*; D006017:Glycolipids / Q000494:pharmacology; D042845:Laccase / Q000378:metabolism*; D012275:Oryza; D010407:Penicillium / Q000187:drug effects / Q000201:enzymology; D019800:Phenol / Q000378:metabolism* / Q000633:toxicity; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000201:enzymology; D012503:Saponins / Q000494:pharmacology; D013501:Surface-Active Agents / Q000494:pharmacology
D001426:Bacterial Proteins; D005656:Fungal Proteins; D006017:Glycolipids; D012503:Saponins; D013501:Surface-Active Agents; C418382:rhamnolipid; D019800:Phenol; D042845:Laccase