Evidence of oxidative stress in relation to feeding type during early life in premature infants.
Morbidity in the premature (PT) infant may reflect difficult adaptation to oxygen. We hypothesized that feeding including formula feeding (F) and feeding mother's milk (HM) with added fortifier would affect redox status. Therefore, 65 PT infants (birth weight: 1146 ± 261 g; GA: 29 ± 2.5 wk; mean ± SD) were followed biweekly, once oral feeds were introduced. Feeding groups: F (>75% total feeds) and HM (>75% total feeds) were further subdivided according to human milk fortifier (HMF) content of 0-19, 20-49, and ≥ 50%. Oxidative stress was quantified by F2-isoprostanes (F2-IsoPs) in urine, protein carbonyls, and oxygen radical absorbance capacity (ORAC) in plasma. F2-IsoPs (ng/mg creatinine): 0-2 wk, 125 ± 63; 3-4 wk, 191 ± 171; 5-6 wk, 172 ± 83; 7-8 wk, 211 ± 149; 9-10 wk, 222 ± 121; and >10 wk, 183 ± 67. Protein carbonyls from highest [2.41 ± 0.75 (n = 9)] and lowest [2.25 ± 0.89 (n = 12) pmol/μg protein] isoprostane groups did not differ. ORAC: baseline, 6778 ± 1093; discharge, 6639 ± 735 [full term 4 and 12 M, 9010 ± 600 mg (n = 12) TE]. Highest isoprostane values occurred in infants with >50% of their mother's milk fortified. Further research on HMF is warranted.
Department of Human Nutritional Sciences, University of Manitoba, Winnipeg, Manitoba R3T 2N2, Canada. frielj@ms.umanitoba.ca
James K
JK
Friel
Bill
B
Diehl-Jones
Kevin A
KA
Cockell
Aaron
A
Chiu
Rasheda
R
Rabanni
Sean S
SS
Davies
L Jackson
LJ
Roberts
D000704:Analysis of Variance; D015415:Biomarkers / Q000097:blood / Q000652:urine; D001903:Bottle Feeding; D001942:Breast Feeding; D002374:Catalase / Q000097:blood; D028441:F2-Isoprostanes / Q000652:urine; D005260:Female; D005865:Gestational Age; D005979:Glutathione Peroxidase / Q000097:blood; D006801:Humans; D041943:Infant Formula; D007231:Infant, Newborn; D007234:Infant, Premature; D019102:Infant, Very Low Birth Weight; D008297:Male; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D010865:Pilot Projects; D050050:Protein Carbonylation; D013482:Superoxide Dismutase / Q000097:blood
D015415:Biomarkers; D028441:F2-Isoprostanes; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase