Plasma membrane Ca²+ transporters mediate virus-induced acquired resistance to oxidative stress.
This paper reports the phenomenon of acquired cross-tolerance to oxidative stress in plants and investigates the activity of specific Ca²+ transport systems mediating this phenomenon. Nicotiana benthamiana plants were infected with Potato virus X (PVX) and exposed to oxidative [either ultraviolet (UV-C) or H₂O₂] stress. Plant adaptive responses were assessed by the combined application of a range of electrophysiological (non-invasive microelectrode ion flux measurements), biochemical (Ca²+- and H+-ATPase activity), imaging (fluorescence lifetime imaging measurements of changes in intracellular Ca²+ concentrations), pharmacological and cytological transmission electrone microscopy techniques. Virus-infected plants had a better ability to control UV-induced elevations in cytosolic-free Ca²+ and prevent structural and functional damage of chloroplasts. Taken together, our results suggest a high degree of crosstalk between UV and pathogen-induced oxidative stresses, and highlight the crucial role of Ca²+ efflux systems in acquired resistance to oxidative stress in plants.
School of Agricultural Science, Menzies Research Institute, University of Tasmania, Private Bag 54, Hobart, Tas 7001, Australia. shabala@utas.edu.au
Sergey
S
Shabala
Lone
L
Baekgaard
Lana
L
Shabala
Anja
A
Fuglsang
Olga
O
Babourina
Michael G
MG
Palmgren
Tracey A
TA
Cuin
Zed
Z
Rengel
Lev G
LG
Nemchinov
D017920:Antiporters / Q000378:metabolism*; D002118:Calcium / Q000032:analysis; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D027682:Cation Transport Proteins / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D002736:Chloroplasts / Q000528:radiation effects / Q000821:virology; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D018384:Oxidative Stress; D010940:Plant Proteins / Q000378:metabolism; D017863:Potexvirus / Q000472:pathogenicity*; D006180:Proton-Translocating ATPases / Q000378:metabolism; D013312:Stress, Physiological; D014026:Nicotiana / Q000201:enzymology / Q000528:radiation effects / Q000821:virology*; D014466:Ultraviolet Rays
D017920:Antiporters; D027682:Cation Transport Proteins; D010940:Plant Proteins; C027614:calcium-hydrogen antiporters; D006861:Hydrogen Peroxide; D006180:Proton-Translocating ATPases; D000252:Calcium-Transporting ATPases; D002118:Calcium