Establishment and evaluation of event-specific quantitative PCR method for genetically modified soybean MON89788.
A novel real-time PCR-based analytical method was established for the event-specific quantification of a GM soybean event MON89788. The conversion factor (C(f)) which is required to calculate the GMO amount was experimentally determined. The quantitative method was evaluated by a single-laboratory analysis and a blind test in a multi-laboratory trial. The limit of quantitation for the method was estimated to be 0.1% or lower. The trueness and precision were evaluated as the bias and reproducibility of the relative standard deviation (RSD(R)), and the determined bias and RSD(R) values for the method were both less than 20%. These results suggest that the established method would be suitable for practical detection and quantification of MON89788.
Analytical Science Division, National Food Research Institute, National Agriculture and Food Research Organization, 2-1-12 Kannondai, Tsukuba, Ibaraki, Japan.
Reona
R
Takabatake
Mari
M
Onishi
Tomohiro
T
Koiwa
Satoshi
S
Futo
Yasutaka
Y
Minegishi
Hiroshi
H
Akiyama
Reiko
R
Teshima
Satoshi
S
Furui
Kazumi
K
Kitta
D005504:Food Analysis / Q000379:methods*; D030841:Food, Genetically Modified; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D013025:Glycine max