Analysis of glycosaminoglycans in stem cell glycomics.
Glycosaminoglycans (GAGs) play a critical role in the binding and activation of growth factors in cell signal transduction required for biological development. A glycomics approach can be used to examine GAG content, composition, and structure in stem cells in order to characterize their general differentiation. Specifically, this method may be used to evaluate chondrogenic differentiations by profiling for the GAG content of the differentiated cells. Here, embryonic-like teratocarcinoma cells, NCCIT, a developmentally pluripotent cell line, were used as a model for establishing GAG glycomic methods, but will be easily transferrable to embryonic stem cell cultures.
Department of Chemistry and Chemical Biology, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY, USA.
Boyangzi
B
Li
Haiying
H
Liu
Zhenqing
Z
Zhang
Hope E
HE
Stansfield
Jonathan S
JS
Dordick
Robert J
RJ
Linhardt
D045744:Cell Line, Tumor; D002469:Cell Separation; D002809:Chondroitin Sulfates / Q000032:analysis / Q000737:chemistry; D002853:Chromatography, Liquid; D003871:Dermatan Sulfate / Q000032:analysis / Q000737:chemistry; D004187:Disaccharides / Q000032:analysis / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D054794:Glycomics / Q000379:methods*; D006025:Glycosaminoglycans / Q000032:analysis* / Q000302:isolation & purification; D006493:Heparin / Q000032:analysis / Q000737:chemistry; D006801:Humans; D013058:Mass Spectrometry; D008858:Microscopy, Phase-Contrast; D058105:Polymerization; D013234:Stem Cells / Q000378:metabolism*
D004187:Disaccharides; D006025:Glycosaminoglycans; D003871:Dermatan Sulfate; D006493:Heparin; D002809:Chondroitin Sulfates