A broad-spectrum, efficient and nontransgenic approach to control plant viruses by application of salicylic acid and jasmonic acid.
Plant viruses cause many diseases that lead to significant economic losses. However, most of the approaches to control plant viruses, including transgenic processes or drugs are plant-species-limited or virus-species-limited, and not very effective. We introduce an application of jasmonic acid (JA) and salicylic acid (SA), a broad-spectrum, efficient and nontransgenic method, to improve plant resistance to RNA viruses. Applying 0.06 mM JA and then 0.1 mM SA 24 h later, enhanced resistance to Cucumber mosaic virus (CMV), Tobacco mosaic virus (TMV) and Turnip crinkle virus (TCV) in Arabidopsis, tobacco, tomato and hot pepper. The inhibition efficiency to virus replication usually achieved up to 80-90%. The putative molecular mechanism was investigated. Some possible factors affecting the synergism of JA and SA have been defined, including WRKY53, WRKY70, PDF1.2, MPK4, MPK2, MPK3, MPK5, MPK12, MPK14, MKK1, MKK2, and MKK6. All genes involving in the synergism of JA and SA were investigated. This approach is safe to human beings and environmentally friendly and shows potential as a strong tool for crop protection against plant viruses.
Ministry of Education Key Laboratory for Bio-Resource and Eco-Environment, College of Life Science, Sichuan University, Chengdu 610064, China.
Jing
J
Shang
De-Hui
DH
Xi
Fei
F
Xu
Shao-Dong
SD
Wang
Sen
S
Cao
Mo-Yun
MY
Xu
Ping-Ping
PP
Zhao
Jian-Hui
JH
Wang
Shu-Dan
SD
Jia
Zhong-Wei
ZW
Zhang
Shu
S
Yuan
Hong-Hui
HH
Lin
D000998:Antiviral Agents / Q000008:administration & dosage / Q000494:pharmacology; D017360:Arabidopsis / Q000821:virology*; D003517:Cyclopentanes / Q000008:administration & dosage / Q000494:pharmacology*; D054883:Oxylipins / Q000008:administration & dosage / Q000494:pharmacology*; D010935:Plant Diseases / Q000821:virology*; D018515:Plant Leaves / Q000187:drug effects / Q000821:virology; D010942:Plant Viruses / Q000187:drug effects*; D020156:Salicylic Acid / Q000008:administration & dosage / Q000494:pharmacology*; D019657:Solanaceae / Q000821:virology*; D014779:Virus Replication / Q000187:drug effects
D000998:Antiviral Agents; D003517:Cyclopentanes; D054883:Oxylipins; C011006:jasmonic acid; D020156:Salicylic Acid