Label free sub-picomole level DNA detection with Ag nanoparticle decorated Au nanotip arrays as surface enhanced Raman spectroscopy platform.
Label free optical sensing of adenine and thymine oligonucleotides has been achieved at the sub-picomole level using self assembled silver nanoparticles (AgNPs) decorated gold nanotip (AuNT) arrays. The platform consisting of the AuNTs not only aids in efficient bio-immobilization, but also packs AgNPs in a three dimensional high surface area workspace, assisting in surface enhanced Raman scattering (SERS). The use of sub-10 nm AgNPs with optimum inter-particle distance ensures amplification of the chemically specific Raman signals of the adsorbed adenine, thymine, cytosine and guanine molecules in SERS experiments. High temporal stability of the Raman signals ensured reliable and repeatable DNA detection even after three weeks of ambient desk-top conservation. This facile architecture, being three dimensional and non-lithographic, differs from conventional SERS platforms.
Department of Materials Science and Engineering, National Chiao-Tung University, Hsinchu 300, Taiwan.
Hung-Chun
HC
Lo
Hsin-I
HI
Hsiung
Surojit
S
Chattopadhyay
Hsieh-Cheng
HC
Han
Chia-Fu
CF
Chen
Jih Perng
JP
Leu
Kuei-Hsien
KH
Chen
Li-Chyong
LC
Chen
D015374:Biosensing Techniques / Q000295:instrumentation; D004247:DNA / Q000032:analysis* / Q000235:genetics*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D008832:Microchemistry / Q000295:instrumentation*; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology / Q000295:instrumentation; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation*; D012834:Silver / Q000737:chemistry*; D013059:Spectrum Analysis, Raman / Q000295:instrumentation*; D013194:Staining and Labeling
D012834:Silver; D004247:DNA