[Effect observed on hepatic egg granuloma inhibitted in mice after using rsj26GST vaccine from Schistosoma japonicum].
OBJECTIVE
To study the anti-schistosomiasis effect in mouse immunized with rSj26GST vaccine.


METHODS
The E.coli BL21/pET28a of rSj26GST gene was coated LB/Kana/IPTG/X-gal pan, cultured, collecting bacteria, bacteria broken, separation, purification. 5 μL purified protein were carried out from SDS-PAGE analysis, then to observe the results of purified protein. Each mouse in infection control group was boosted with 50 μL PBS on 0 weeks later, 2 weeks later and 4 weeks later. While each mice rSj26GST vaccine group was boosted with rSj26GST vaccine. Two weeks after last immunization, each mouse was challenged with 30 ± 2 cercariae of S. japonicum Chinese strain. The control group wasn't do some treatment. The mice were sacrificed at the 45th day post-infection. The size of single egg granulomas in livers was measured with micrometer. The level of hyaluronic acid (HA) and laminin (LN) in sera of the mice was determined by ELISA.


RESULTS
The mean hepatic egg granuloma diameters of the rSj26GST vaccine group(191.6 ± 26.3) μm was significantly smaller than that in infection control group (267.7 ± 28.6) μm (P<0.05). The level of HA and LN in sera of rSj26GST vaccine vaccinated mice was markedly lower than in infection control group (P<0.05).


CONCLUSIONS
The results showed that rSj26GST vaccine may act as an effective inhibitor against formation of egg granuloma and reduce immunopathological damage caused by Schistosoma japonicum in the host.
Medicine College of Northwest University for Nationalities, Lanzhou 730030, China.
Jian
J
Du
Jing
J
Li
Xiu-luo
XL
Fang
Zhi-min
ZM
Jing
D000818:Animals; D005260:Female; D005982:Glutathione Transferase / Q000276:immunology; D006099:Granuloma / Q000517:prevention & control*; D008107:Liver Diseases / Q000517:prevention & control*; D051379:Mice; D008807:Mice, Inbred BALB C; D010063:Ovum; D012554:Schistosomiasis japonica / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000276:immunology
D014614:Vaccines, Synthetic; D005982:Glutathione Transferase