TH17 is involved in the remarkable regression of metastatic malignant melanoma to topical diphencyprone.
The authors provide an update on a previously reported patient with in-transit metastatic melanoma of the scalp treated with topical diphencyprone (DPCP). Molecular studies implicate the thymus-derived TH17 lymphocyte subset in a remarkable immunotherapeutic regression. The authors performed RT-PCR of total RNA from paraffin-embedded tissue before and after treatment with DPCP. Before treatment with DPCP, the authors found elevated expression of IL 17C/D/E/F; after treatment there was no detectable expression. Conversely, increased expression of PLZF/CD27 and CTLA4 was seen after treatment with no expression before treatment. No expression of IL17A/B, CD7, RORgTand FoxP3 were before or after treatment. Conclusions are limited to only the time samples were obtained. Remarkable regression of an in-transit metastatic melanoma treated with the immunomodulatory agent DPCP showed gain and loss of gene expression of the TH17 pathway. Further study of this pathway from NK to NK-T to TH7 and TH1 cells both with and without accessory or dendritic cells will improve understanding of contact sensitizers as topical immunomodulators.
Department of Medicine-Pulmonary Division, University of Sydney and Royal Prince Alfred Hospital, Sydney, Australia.
Frank
F
Martiniuk
Diona L
DL
Damian
John F
JF
Thompson
Richard A
RA
Scolyer
Kam-Meng
KM
Tchou-Wong
William R
WR
Levis
D000287:Administration, Topical; D000368:Aged; D015703:Antigens, CD / Q000235:genetics; D060908:CTLA-4 Antigen; D003521:Cyclopropanes / Q000008:administration & dosage*; D006241:Haptens / Q000008:administration & dosage*; D006801:Humans; D020381:Interleukin-17 / Q000235:genetics / Q000378:metabolism; D051741:Kruppel-Like Transcription Factors / Q000235:genetics; D008297:Male; D008545:Melanoma / Q000188:drug therapy* / Q000235:genetics / Q000276:immunology / Q000556:secondary; D009824:Ointments; D000075722:Promyelocytic Leukemia Zinc Finger Protein; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012535:Scalp; D012878:Skin Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000276:immunology / Q000473:pathology; D058504:Th17 Cells / Q000276:immunology*
D015703:Antigens, CD; D060908:CTLA-4 Antigen; C556706:CTLA4 protein, human; D003521:Cyclopropanes; D006241:Haptens; D020381:Interleukin-17; D051741:Kruppel-Like Transcription Factors; D009824:Ointments; D000075722:Promyelocytic Leukemia Zinc Finger Protein; C092037:ZBTB16 protein, human; C029402:diphenylcyclopropenone