Use of integrin-linked kinase to extend function of encapsulated pancreatic tissue.
We have studied the impact of overexpression of an intracellular signaling protein, integrin-linked kinase (ILK), on the survival and function of encapsulated islet tissue used for the treatment of type 1 diabetes. The dimensions of the encapsulated tissue can impact the stresses placed on the tissue and ILK overexpression shows the ability to extend function of dissociated cells as well as intact islets. These results suggest that lost cell-extracellular matrix interactions in cell encapsulation systems can lead to decreased insulin secretion and ILK signaling is a target to overcome this phenomenon.
Department of Chemical Engineering, University of South Carolina, Columbia, SC, USA. blanchej@cec.sc.edu
James O
JO
Blanchette
Steven J
SJ
Langer
Suchit
S
Sahai
Pritesh S
PS
Topiwala
Leslie L
LL
Leinwand
Kristi S
KS
Anseth
D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation; D002470:Cell Survival; D007515:Islets of Langerhans / Q000166:cytology* / Q000502:physiology*; D016381:Islets of Langerhans Transplantation / Q000379:methods*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009924:Organ Culture Techniques / Q000379:methods*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D014162:Transfection / Q000379:methods; D000099200:Scaffold Protein ILK
D017346:Protein Serine-Threonine Kinases; D011994:Recombinant Proteins; D000099200:Scaffold Protein ILK