[miRNA changes in the reverse remodeling heart of rats].
OBJECTIVE
To establish a reverse remodeling heart model in rats and observe collagen and TGF-β expression and relevant microRNAs changes during reverse remodeling.


METHODS
Lewis rats were divided into four groups including sham (NL, n = 10), abdominal aortic constriction (AAC, n = 10), heterotopic transplantation of abdominal aortic constriction (AAC-HT, n = 9) and heterotopic transplantation of normal heart (HT, n = 8). Left ventricular wall thickness and LV cavity were measured by echocardiography. The cardiomyocyte cross-sectional area (CSA) was determined on HE stained sections. Immunohistochemical and qRT-PCR were used to detect collagen and TGF-β expressions. miRNAs were detected by MicroRNA microarray.


RESULTS
Heart weight, left ventricular wall thickness and CSA were significantly increased in AAC hearts compared to those in the NL and AAC-HT hearts. The collagen and TGF-β were increased in AAC hearts and further increased in AAC-HT hearts. miRNA microarray evidenced more than two folds changes on 82 miRNAs compared to NL (10 in AAC, 32 in AAC-HT and 40 in HT).


CONCLUSIONS
Rat abdominal aortic constriction and heterotopic transplantation could be used as a reverse remodeling heart model and significant collagen and TGF-β as well microRNA expression changes were evidenced in this model.
Key Laboratory of Cardiovascular Regenerative Medicine, Cardiovascular Institute and Fu Wai Hospital, Chinese Academy of Medical Science, Peking Union Medicine College, Beijing 100037, China.
Jue
J
Wang
Zhe
Z
Zheng
Fu-Qian
FQ
Lin
Cheng-Chao
CC
Sun
Rui-Xia
RX
Xu
Sheng-Shou
SS
Hu
D000818:Animals; D003094:Collagen / Q000378:metabolism; D006352:Heart Ventricles / Q000378:metabolism*; D008297:Male; D035683:MicroRNAs / Q000378:metabolism*; D032383:Myocytes, Cardiac / Q000378:metabolism; D051381:Rats; D011917:Rats, Inbred Lew; D016212:Transforming Growth Factor beta / Q000378:metabolism; D020257:Ventricular Remodeling
D035683:MicroRNAs; D016212:Transforming Growth Factor beta; D003094:Collagen