Study of the interaction between the amyloid beta peptide (1-40) and antioxidant compounds by nuclear magnetic resonance spectroscopy.
Amyloid beta peptide (Abeta) aggregation leads to the senile plaque formation, a process that is strongly influenced by oxidative stress and is considered as the molecular basis of various neurodegenerative diseases, such as Alzheimer's disease (AD). Endogenous antioxidants or dietary derived compounds may down-regulate this process. In this study, the interaction of two antioxidants, oleuropein (OE) and melatonin (M), with Abeta is monitored through nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry. The concerted application of these two analytical techniques provides new experimental evidence and residue-specific insights into the interacting Abeta peptide amino acids that are implicated in this process. Both antioxidant compounds interact in a similar way with the peptide and cause chemical shift variations. The most pronounced resonance changes have been observed for the 1H-15N signals of N-terminal region and Leu17-Phe20 residues, as monitored by NMR titration studies.
Department ofPharmacy, University ofPatras, Panepistimioupoli, Rion, GR 26504, Greece.
Petros A
PA
Galanakis
Fotini N
FN
Bazoti
Jonas
J
Bergquist
Karin
K
Markides
Georgios A
GA
Spyroulias
Anthony
A
Tsarbopoulos
D000544:Alzheimer Disease / Q000378:metabolism; D016229:Amyloid beta-Peptides / Q000737:chemistry* / Q000378:metabolism; D000975:Antioxidants / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D057888:Iridoid Glucosides; D039823:Iridoids; D008550:Melatonin / Q000737:chemistry* / Q000378:metabolism; D019906:Nuclear Magnetic Resonance, Biomolecular; D010446:Peptide Fragments / Q000737:chemistry* / Q000378:metabolism; D011714:Pyrans / Q000737:chemistry* / Q000378:metabolism
D016229:Amyloid beta-Peptides; D000975:Antioxidants; D057888:Iridoid Glucosides; D039823:Iridoids; D010446:Peptide Fragments; D011714:Pyrans; C078592:amyloid beta-protein (1-40); C002769:oleuropein; D008550:Melatonin