High-resolution mapping of interstitial telomeric repeats in Syrian hamster metaphase chromosomes.
Karyotype analysis of the Syrian hamster (Mesocricetus auratus) was performed after DAPI-banding of metaphase chromosomes obtained from cultivated skin fibroblasts of a newborn animal. Fluorescence in situ hybridization with telomeric FITC-conjugated peptide nucleic acid probe was applied to map interstitial blocks of (TTAGGG)(n) repeats. Strong fluorescence in situ hybridization signals corresponded to interstitial telomeric repeats in pericentromeric chromatin bands of chromosomes 2, 4, 14, 20, and X. High-resolution DAPI-banding allowed specifying the arrangement of bands in the pericentromeric regions of these chromosomes.
Institute of Cytology, Russian Academy of Sciences, St. Petersburg, Russia.
S
S
Demin
N
N
Pleskach
M
M
Svetlova
L
L
Solovjeva
D000818:Animals; D000831:Animals, Newborn; D001483:Base Sequence; D002478:Cells, Cultured; D002871:Chromosome Banding; D002874:Chromosome Mapping / Q000379:methods*; D033481:Chromosomes, Mammalian / Q000235:genetics*; D006224:Cricetinae; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D008647:Mesocricetus / Q000235:genetics*; D008677:Metaphase / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D016615:Telomere / Q000235:genetics