CCR7 is involved in the migration of neutrophils to lymph nodes.
Increasing evidence suggests that neutrophils may participate in the regulation of adaptive immune responses, and can reach draining lymph nodes and cross-prime naive T cells. The aim of this study was to identify the mechanism(s) involved in the migration of neutrophils to the draining lymph nodes. We demonstrate that a subpopulation of human and mouse neutrophils express CCR7. CCR7 is rapidly expressed at the membrane upon stimulation. In vitro, stimulated human neutrophils migrate in response to the CCR7 ligands CCL19 and CCL21. In vivo, injection of complete Freund adjuvant induces a rapid recruitment of neutrophils to the lymph nodes in wild-type mice but not in Ccr7(-/-) mice. Moreover, intradermally injected interleukin-17-and granulocyte-macrophage colony-stimulating factor-stimulated neutrophils from wild-type mice, but not from Ccr7(-/-) mice, migrate to the draining lymph nodes. These results identify CCR7 as a chemokine receptor involved in the migration of neutrophils to the lymph nodes.
INSERM Unité 892, Centre de Recherche en Cancérologie Nantes-Angers, 4 rue Larrey, Angers, France.
Céline
C
Beauvillain
Pierre
P
Cunin
Andrea
A
Doni
Mari
M
Scotet
Sébastien
S
Jaillon
Marie-Line
ML
Loiry
Giovanni
G
Magistrelli
Krzysztof
K
Masternak
Alain
A
Chevailler
Yves
Y
Delneste
Pascale
P
Jeannin
D000818:Animals; D002465:Cell Movement / Q000235:genetics* / Q000276:immunology; D002478:Cells, Cultured; D002634:Chemotaxis, Leukocyte / Q000235:genetics*; D006801:Humans; D008198:Lymph Nodes / Q000166:cytology* / Q000276:immunology / Q000378:metabolism / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D020556:Neutrophil Infiltration / Q000235:genetics; D009504:Neutrophils / Q000276:immunology / Q000378:metabolism / Q000502:physiology*; D054400:Receptors, CCR7 / Q000235:genetics / Q000378:metabolism / Q000502:physiology*
C515371:Ccr7 protein, mouse; D054400:Receptors, CCR7