Hemoglobin determination with paired emitter detector diode.
Two ordinary green light-emitting diodes used as light emitter and detector coupled with simple voltmeter form a complete, cost-effective prototype of a photometric hemoglobinometer. The device has been optimized for cuvette assays of total hemoglobin (Hb) in diluted blood using three different chemical methods recommended for the needs of clinical analysis (namely Drabkin, lauryl sulfate, and dithionite methods). The utility of developed device for real analytics has been validated by the assays of total Hb content in human blood. The results of analysis are fully compatible with those obtained using clinically recommended method and clinical analyzer.
University of Warsaw, Department of Chemistry, Pasteura 1, 02-093 Warsaw, Poland.
Elżbieta
E
Mieczkowska
Robert
R
Koncki
Łukasz
Ł
Tymecki
D004867:Equipment Design; D006454:Hemoglobins / Q000032:analysis*; D006801:Humans; D010783:Photometry / Q000191:economics / Q000295:instrumentation / Q000379:methods*
D006454:Hemoglobins