Crystallization and preliminary X-ray diffraction analysis of red clover necrotic mosaic virus.
Red clover necrotic mosaic virus (RCNMV) is a species that belongs to the Tombusviridae family of plant viruses with a T = 3 icosahedral capsid. RCNMV virions were purified and were crystallized for X-ray analysis using the hanging-drop vapor-diffusion method. Self-rotation functions and systematic absences identified the space group as I23, with two virions in the unit cell. The crystals diffracted to better than 4 Å resolution but were very radiation-sensitive, causing rapid decay of the high-resolution reflections. The data were processed to 6 Å in the analysis presented here.
Department of Plant Pathology, North Carolina State University, Raleigh, North Carolina, USA.
Stanton L
SL
Martin
Richard H
RH
Guenther
Tim L
TL
Sit
Paul D
PD
Swartz
Flora
F
Meilleur
Steven A
SA
Lommel
Robert B
RB
Rose
D003460:Crystallization; D018360:Crystallography, X-Ray; D046529:Microscopy, Electron, Transmission; D019183:Tombusviridae / Q000737:chemistry* / Q000648:ultrastructure; D014771:Virion / Q000737:chemistry* / Q000648:ultrastructure