Change in wall composition of transfer and aleurone cells during wheat grain development.
In addition to the starchy endosperm, a specialized tissue accumulating storage material, the endosperm of wheat grain, comprises the aleurone layer and the transfer cells next to the crease. The transfer cells, located at the ventral region of the grain, are involved in nutrient transfer from the maternal tissues to the developing endosperm. Immunolabeling techniques, Raman spectroscopy, and synchrotron infrared micro-spectroscopy were used to study the chemistry of the transfer cell walls during wheat grain development. The kinetic depositions of the main cell wall polysaccharides of wheat grain endosperm, arabinoxylan, and (1-3)(1-4)-β-glucan in transfer cell walls were different from kinetics previously observed in the aleurone cell walls. While (1-3)(1-4)-β-glucan appeared first in the aleurone cell walls at 90°D, arabinoxylan predominated in the transfer cell walls from 90 to 445°D. Both aleurone and transfer cell walls were enriched in (1-3)(1-4)-β-glucan at the mature stage of wheat grain development. Arabinoxylan was more substituted in the transfer cell walls than in the aleurone walls. However, arabinoxylan was more feruloylated in the aleurone than in the transfer cell walls, whatever the stage of grain development. In the transfer cells, the ferulic acid was less abundant in the outer periclinal walls while para-coumarate was absent. Possible implications of such differences are discussed.
INRA, UR1268 Biopolymères Interactions Assemblages, 44300, Nantes, France. paul.robert@nantes.inra.fr
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Robert
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Jamme
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Barron
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Bouchet
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Saulnier
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Dumas
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Guillon
D002454:Cell Differentiation; D002473:Cell Wall / Q000378:metabolism*; D002523:Edible Grain / Q000254:growth & development / Q000378:metabolism*; D056625:Endosperm / Q000166:cytology / Q000378:metabolism*; D011134:Polysaccharides / Q000378:metabolism*; D017550:Spectroscopy, Fourier Transform Infrared; D013059:Spectrum Analysis, Raman; D014908:Triticum / Q000378:metabolism*; D014990:Xylans / Q000032:analysis
D011134:Polysaccharides; D014990:Xylans; C085118:arabinoxylan