Fabrication of individual scaffolds based on a patient-specific alveolar bone defect model.
Fabricating individualized tissue engineering scaffolds based on the three-dimensional shape of patient bone defects is required for the successful clinical application of bone tissue engineering. However, there are currently no reported studies of individualized bone tissue engineering scaffolds that truly reproduce a patient-specific bone defect. We fabricated individualized tissue engineering scaffolds based on alveolar bone defects. The individualized poly(lactide-co-glycolide) and tricalcium phosphate composite scaffolds were custom-made by acquiring the three-dimensional model through computed tomography, which was input into the computer-aided low-temperature deposition manufacturing system. The three-dimensional shape of the fabricated scaffold was identical to the patient-specific alveolar bone defects, with an average macropore diameter of 380 μm, micropore diameters ranging from 3 to 5 μm, and an average porosity of 87.4%. The mechanical properties of the scaffold were similar to adult cancellous bone. Scaffold biocompatibility was confirmed by attachment and proliferation of human bone marrow mesenchymal stem cells. Successful realization of individualized scaffold fabrication will enable clinical application of tissue-engineered bone at an early date.
Department of Prosthodontics, School of Stomatology, Capital Medical University, Tian Tan Xi Li No. 4, Beijing 100050, China.
Jinghui
J
Li
Lei
L
Zhang
Shengyi
S
Lv
Shengjie
S
Li
Na
N
Wang
Zhenting
Z
Zhang
D000328:Adult; D016301:Alveolar Bone Loss / Q000628:therapy*; D001854:Bone Marrow Cells; D001861:Bone Regeneration; D002130:Calcium Phosphates / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D017076:Computer-Aided Design; D005260:Female; D006801:Humans; D007568:Jaw / Q000033:anatomy & histology*; D019344:Lactic Acid / Q000494:pharmacology; D008297:Male; D008422:Materials Testing; D059630:Mesenchymal Stem Cells; D008855:Microscopy, Electron, Scanning; D008954:Models, Biological; D011100:Polyglycolic Acid / Q000494:pharmacology; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D016062:Porosity; D011474:Prosthesis Design; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
D002130:Calcium Phosphates; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; C018392:tricalcium phosphate