Sustained release of nucleic acids from polymeric nanoparticles using microemulsion precipitation in supercritical carbon dioxide.
A general approach for producing biodegradable nanoparticles for sustained nucleic acid release is presented. The nanoparticles are produced by precipitating a water-in-oil microemulsion in supercritical CO(2). The microemulsion consists of a transfer RNA aqueous solution (water phase), dichloromethane containing poly(l-lactic acid)-poly(ethylene glycol) (oil phase), the surfactant n-octyl β-D-glucopyranoside, and the cosurfactant n-butanol.
Department of Chemistry, Stanford University, 333 Campus Drive, Stanford, CA 94305-5080, USA.
Jun
J
Ge
Gunilla B
GB
Jacobson
Tatsiana
T
Lobovkina
Krister
K
Holmberg
Richard N
RN
Zare
D002245:Carbon Dioxide / Q000737:chemistry*; D004655:Emulsions; D006863:Hydrogen-Ion Concentration; D007773:Lactates / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D012343:RNA, Transfer / Q000737:chemistry*; D013863:Thioglucosides / Q000737:chemistry
D004655:Emulsions; D007773:Lactates; D013863:Thioglucosides; C088513:poly(lactic acid-ethylene glycol); D002245:Carbon Dioxide; D011092:Polyethylene Glycols; C042989:n-octyl-beta-D-thioglucopyranoside; D012343:RNA, Transfer