Comparative metabolic profiling of paediatric ependymoma, medulloblastoma and pilocytic astrocytoma.
Brain tumours are the most common solid tumours in children and a major cause of childhood mortality. The most common paediatric brain tumours include ependymomas, cerebellar astrocytomas and medulloblastomas. These brain tumours are highly heterogeneous regarding their histology, prognosis and therapeutic response. Subtle biochemical changes can be detected in intact tissues by High-Resolution Proton Magnetic Angle Spinning Spectroscopy (HR-MAS) revealing the status of tumour microheterogeneity and metabolic alterations before they are morphologically detectable. In this study, we present metabolic profiles by HR-MAS of 20 intact tissue samples from paediatric brain tumours. Tumour types include ependymoma, medulloblastoma and pilocytic astrocytoma. The metabolic characterization of paediatric brain tumour tissue by HR-MAS spectroscopy provided differential patterns for these tumours. The metabolic composition of the tumour tissue was highly consistent with previous in vivo and ex vivo studies. Some resonances detected in this work and not previously observed by in vivo spectroscopy also show potential in determining tumour type and grade (fatty acids, phenylalanine, glutamate). Overall, this work suggests that the additional information obtained by NMR metabolic profiling applied to tissue from paediatric brain tumours may be useful for assessing tumour grade and determining optimum treatment strategies.
Instituto de Física Gleb Wataghin, Universidade Estadual de Campinas, UNICAMP, Brazil.
S
S
Cuellar-Baena
J M
JM
Morales
H
H
Martinetto
J
J
Calvar
G
G
Sevlever
G
G
Castellano
M
M
Cerdá-Nicolás
B
B
Celda
D
D
Monleon
D000596:Amino Acids / Q000737:chemistry; D000704:Analysis of Variance; D001923:Brain Chemistry; D001932:Brain Neoplasms / Q000378:metabolism* / Q000473:pathology; D002648:Child; D002675:Child, Preschool; D005227:Fatty Acids / Q000737:chemistry; D005910:Glioma / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007223:Infant; D008279:Magnetic Resonance Imaging; D055432:Metabolomics / Q000379:methods*; D025341:Principal Component Analysis
D000596:Amino Acids; D005227:Fatty Acids