Fluorescence detection of trace PCB101 based on PITC immobilized on porous AAO membrane.
A sensitive and selective fluorescent membrane for rapid detection of trace 2,2',4,5,5'-pentachlorinated biphenyl (PCB101) has been achieved by immobilizing the fluorophore phenyl isothiocyanate (PITC) onto porous anodic aluminium oxide (AAO) membrane (denoted as PITC@AAO). The fluorescence of the PITC@AAO membrane is obviously enhanced after titrating the analyte PCB101 into the membrane, being ascribed to the halogen-bonding interaction between the fluorophore PITC and the analyte PCB101. The fluorescence intensity increases with the PCB101 concentration in the low range below 1 ppm, and there exists an approximate linear relationship between the relative fluorescence intensity and the PCB101 concentration in the low range of 1-6 ppb. Moreover, the PITC@AAO membrane shows good selectivity; for example, it is insensitive to common structural analogs (polychlorinated aromatics). The mechanisms of the fluorescence enhancement and the better sensitivity and selectivity of the PITC@AAO membrane to PCB101 than that of PITC/n-hexane solution are also discussed. This work demonstrates that trace (in ppb range) PCBs can be detected by simple fluorescence measurement.
Key Laboratory of Materials Physics, Institute of Solid State Physics, Chinese Academy of Sciences, Hefei 230031, People's Republic of China.
Meiling
M
Wang
Guowen
G
Meng
Qing
Q
Huang
Mingtao
M
Li
Zhongbo
Z
Li
Chaolong
C
Tang
D000537:Aluminum Oxide / Q000737:chemistry*; D004566:Electrodes; D004785:Environmental Pollutants / Q000032:analysis*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry; D017879:Isothiocyanates / Q000737:chemistry*; D008567:Membranes, Artificial; D011078:Polychlorinated Biphenyls / Q000032:analysis*; D016062:Porosity; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence
D004785:Environmental Pollutants; D005456:Fluorescent Dyes; D017879:Isothiocyanates; D008567:Membranes, Artificial; C005441:phenylisothiocyanate; C009828:2,4,5,2',5'-pentachlorobiphenyl; D011078:Polychlorinated Biphenyls; D000537:Aluminum Oxide