[Growth characteristics, biofilm formation, and antilysozyme activity of Bifidobacterium bifidum after application of chemical analogues of alkyloxybenzenes].
OBJECTIVE
To study the effects of alkyloxybenzenes chemical analogues C7-AOB and C12-AOB on growth properties, biofilm formation and antilysozyme activity of bifidobacteria.


METHODS
Using manufacture strain B. bifidum No. 791 ("Bifidumbacterin", Ecopolis, Kovrov), effects of C7-AOB and C12-AOB were studied. Alkyloxybenzenes were introduced in nutrient medium containing microorganisms, which were cultivated until stationary phase. Clones isolation was performed by seeding of 24-h broth culture on solid nutrient medium. Antilysozyme activity, biofilm formation and growth characteristics were assessed by photometric method (Bukharin O.V., 1999; O'Tool, 2002). Results were statistically treated using Simpson's and Shannon's indexes.


RESULTS
It was established that, unlike methyresorcin, hexylresorcin stimulated growth of B. bifidum and promoted considerable growth of clones with high antilysozyme activity and biofilm formation ability. Dose-response relationship was noted for both compounds: increase of alkyloxybenzene concentration led to more profound changes in B. bifidum population structure.


CONCLUSIONS
Obtained data explain possible mechanisms of gut colonization by bifidobacteria including enhanced growth, antilysozyme activity and biofilm formation by normal flora that promotes enhancement of host defense.
N B
NB
Perunova
S V
SV
Gordeeva
O V
OV
Bukharin
D001555:Benzene Derivatives / Q000494:pharmacology*; D001644:Bifidobacterium / Q000187:drug effects* / Q000254:growth & development*; D018441:Biofilms / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D009113:Muramidase / Q000037:antagonists & inhibitors*; D012118:Resorcinols / Q000494:pharmacology*
D001555:Benzene Derivatives; D012118:Resorcinols; D009113:Muramidase