[Protection of CSE/H2S system in hepatic ischemia reperfusion injury in rats].
OBJECTIVE
To study the protective function and pathophysiology of cystathionine gamma-lyase (CSE)/hydrogen sulfide (H(2)S) system in hepatic ischemia-reperfusion injury (HIRI) in rats.


METHODS
Wistar rats were randomly distributed into sham group (n = 18), ischemia-reperfusion (IR) group (n = 18), IR + NaHS group (n = 18) and IR + DL-propargylglycine (PAG) group (n = 18). The hepatic IR model was established by Pringle's hepatic vascular occlusion. At each of the indicated time points (1, 3 and 6 hours after IR), the serum levels of H(2)S and the hepatic CSE activity were measured. The serum levels of inflammatory factors, including TNF-α, IL-10 were determined by ELISA methods. The expression of apoptotic protein, TNF-α, in liver tissue was tested by Western blot assay, cell apoptosis was examined by TUNEL and the histological changes were examined in each group.


RESULTS
The serum levels of H(2)S and CSE activity were significantly increased in group IR compared with group sham at all indicated time points (P < 0.05). The serum level of inflammatory factors (P < 0.01) and the hepatic expression of TNF-α protein (P < 0.05) were elevated obviously in group IR than that in group sham. Administration of NaHS could reduce the production of inflammatory factors in serum (P < 0.01), inhibit hepatic protein expression of TNF-α (P < 0.05) and attenuate the liver histological scores of IR injury (P < 0.05), whereas PAG aggravated them.


CONCLUSIONS
The endogenous CSE/H(2)S system maybe involved in the pathogenesis of hepatic IR injury, which suggests that CSE/H(2)S system can protect liver from IR injury in rats by intervening in inflammatory reaction, attenuating the injury severity and inhibiting expression of apoptotic protein TNF-α.
Department of ICU, First Clinical Medical School of Harbin Medical University, Harbin 150001, China.
Kai
K
Kang
Hong-chi
HC
Jiang
Ming-yan
MY
Zhao
Xue-ying
XY
Sun
Shang-ha
SH
Pan
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D003542:Cystathionine gamma-Lyase / Q000097:blood / Q000502:physiology*; D004195:Disease Models, Animal; D006862:Hydrogen Sulfide / Q000097:blood*; D016753:Interleukin-10 / Q000097:blood; D008099:Liver / Q000098:blood supply* / Q000378:metabolism / Q000473:pathology; D008297:Male; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology* / Q000517:prevention & control; D013440:Sulfides / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D013440:Sulfides; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D003542:Cystathionine gamma-Lyase; C025451:sodium bisulfide; D006862:Hydrogen Sulfide