Combined neutron and X-ray diffraction studies of DNA in crystals and solutions.
Recent developments in instrumentation and facilities for sample preparation have resulted in sharply increased interest in the application of neutron diffraction. Of particular interest are combined approaches in which neutron methods are used in parallel with X-ray techniques. Two distinct examples are given. The first is a single-crystal study of an A-DNA structure formed by the oligonucleotide d(AGGGGCCCCT)(2), showing evidence of unusual base protonation that is not visible by X-ray crystallography. The second is a solution scattering study of the interaction of a bisacridine derivative with the human telomeric sequence d(AGGGTTAGGGTTAGGGTTAGGG) and illustrates the differing effects of NaCl and KCl on this interaction.
European Synchrotron Radiation Facility, 6 Rue Jules Horowitz, 38043 Grenoble, France.
Ricardo M F
RM
Leal
Shirley
S
Callow
Phil
P
Callow
Matthew P
MP
Blakeley
Christine J
CJ
Cardin
William A
WA
Denny
Susana C M
SC
Teixeira
Edward P
EP
Mitchell
V Trevor
VT
Forsyth
D000166:Acridines / Q000737:chemistry / Q000378:metabolism; D003460:Crystallization; D044704:DNA, A-Form / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D008958:Models, Molecular; D033363:Neutron Diffraction; D009502:Neutrons; D011189:Potassium Chloride / Q000494:pharmacology; D053838:Scattering, Small Angle; D012965:Sodium Chloride / Q000494:pharmacology; D012996:Solutions; D016615:Telomere / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D014961:X-Ray Diffraction
D000166:Acridines; D044704:DNA, A-Form; D012996:Solutions; D012965:Sodium Chloride; D011189:Potassium Chloride