Development of an injectable chitosan/marine collagen composite gel.
A chitosan/marine-originated collagen composite has been developed. This composite gel was characterized and its biocompatibility, as well as an inflammatory reaction, was observed. The chitosan gel including N-3-carboxypropanoil-6-O-(carboxymethyl) chitosan of 3 mol%, 6-O-(carboxymethyl) chitosan of 62 mol% and 6-O-(carboxymethyl) chitin of 35 mol% was prepared and compounded with the salmon atelocollagen (SA) gel at different mixture ratios. The composite gels were injected subcutaneously in to the back of rats. The specimens were harvested for a histological survey as well as a tumor necrosis factor-alpha (TNF-α) assay by ELISA. The inflammatory cell infiltration and release of TNF-α were successively controlled low with the ratio of SA to chitosan at 10:90 or 20:80. The SA gel first, within 2 weeks, and then chitosan in the composite gel were slowly absorbed after implantation, followed by soft tissue formation. It is expected that this composite gel will be available as a carrier for tissue filler and drug delivery systems.
Department of Inorganic Materials, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda, Tokyo 101-0062, Japan.
Wei
W
Wang
Soichiro
S
Itoh
Tomoyasu
T
Aizawa
Atsushi
A
Okawa
Katsuyoshi
K
Sakai
Tsuneo
T
Ohkuma
Makoto
M
Demura
D000818:Animals; D001672:Biocompatible Materials / Q000008:administration & dosage* / Q000138:chemical synthesis*; D048271:Chitosan / Q000008:administration & dosage* / Q000737:chemistry*; D003094:Collagen / Q000008:administration & dosage* / Q000737:chemistry*; D003238:Connective Tissue / Q000187:drug effects / Q000276:immunology*; D016207:Cytokines / Q000276:immunology; D005782:Gels / Q000008:administration & dosage / Q000737:chemistry; D007279:Injections, Subcutaneous; D008422:Materials Testing; D051381:Rats; D017208:Rats, Wistar; D012474:Salmon
D001672:Biocompatible Materials; D016207:Cytokines; D005782:Gels; D003094:Collagen; D048271:Chitosan