Mitochondrial DNA polymorphism of the yeast Saccharomyces bayanus var. uvarum.
Genetic relationships among forty-one strains of Saccharomyces bayanus var. uvarum isolated in different wine regions of Europe and four wild isolates were investigated by restriction analysis (RFLP) of mitochondrial DNA (mtDNA) with four restriction endonucleases, AluI, DdeI, HinfI and RsaI. No clear correlation between origin and source of isolation of S. bayanus var. uvarum strains and their mtDNA restriction profiles was found. On the whole, the mtDNA of S. bayanus var. uvarum is much less polymorphic than that of S. cerevisiae. This observation is in good agreement with results obtained by electrophoretic karyotyping. Unlike wine S cerevisiae, strains of S. bayanus var. uvarum display a low level of chromosome length polymorphism.
State Research Institute for Genetics and Selection of Industrial Microorganisms, Pervyi Dorozhnyipmezd I, Moscow 117545, Russia.
E S
ES
Naumova
G I
GI
Naumov
E
E
Barrio
A
A
Querol
D004271:DNA, Fungal / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics*; D005060:Europe; D010802:Phylogeny; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D012440:Saccharomyces / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D027843:Vitis / Q000382:microbiology; D014920:Wine / Q000382:microbiology
D004271:DNA, Fungal; D004272:DNA, Mitochondrial