Detection of a complementary couple of single-stranded DNAs by use of a quartz crystal device for determination of bacteria.
Preliminary experiments were carried out for the DNA detection targeting a complementary couple of single-stranded DNAs originating from Nitrobacter winogradskyi. The target DNAs were detected successfully with a quartz crystal unit bearing probe DNAs, based on the oscillation frequency decrease due to hybridization of the probe and target DNAs.
Department of Bioengineering, Faculty of Engineering, Nagaoka University of Technology, 1603-1 Kamitomioka-machi, Nagaoka 940-2188, Japan.
Kichiro
K
Kon
Takashi
T
Kuwahara
Masato
M
Shimomura
D001419:Bacteria / Q000302:isolation & purification*; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D015342:DNA Probes / Q000737:chemistry; D004277:DNA, Single-Stranded / Q000737:chemistry / Q000302:isolation & purification*; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods; D009693:Nucleic Acid Hybridization / Q000379:methods; D011791:Quartz / Q000737:chemistry*
D015342:DNA Probes; D004277:DNA, Single-Stranded; D011791:Quartz