Highly efficient production of D-lactate by Sporolactobacillus sp. CASD with simultaneous enzymatic hydrolysis of peanut meal.
Highly efficient D-lactate production by Sporolactobacillus sp. strain CASD was demonstrated in this study. Peanut meal was found to be a better nutrient than yeast extract, soybean meal, soybean peptone, corn steep, liquor beef extract, and ammonium sulfate in the production of D-lactate. To improve the utilization of peanut meal, the material was enzymatically hydrolyzed and simultaneously utilized as the nitrogen source in D-lactate fermentation. Very high D-lactate production (207 g/L) was obtained using 40 g/L of peanut meal in 30-L fed-batch fermentation, with the average productivity of 3.8 g/(L·h) and optical purity of 99.3%. The production of such a high concentration of optically pure D-lactate by strain CASD, with the simultaneous enzymatic hydrolysis of peanut meal and fermentation, represents a new cost-efficient and integrated method for D-lactate production using agricultural by-products.
Institute of Microbiology, Chinese Academy of Sciences, Beijing 100190, People's Republic of China.
Limin
L
Wang
Bo
B
Zhao
Fengsong
F
Li
Ke
K
Xu
Cuiqing
C
Ma
Fei
F
Tao
Qinggang
Q
Li
Ping
P
Xu
D010367:Arachis / Q000378:metabolism*; D056449:Bacillales / Q000201:enzymology* / Q000378:metabolism*; D004798:Enzymes / Q000378:metabolism; D005285:Fermentation; D006868:Hydrolysis; D019344:Lactic Acid / Q000378:metabolism*; D009584:Nitrogen / Q000378:metabolism
D004798:Enzymes; D019344:Lactic Acid; D009584:Nitrogen