Human graft cornea and laser incisions imaging with micrometer scale resolution full-field optical coherence tomography.
Micrometer scale resolution full-field optical coherence tomography (FF-OCT) is developed for imaging human graft corneas. Three-dimensional (3-D) images with ultrahigh resolution (respectively, 1 and 1.5 μm in the axial and transverse directions), comparable to traditional histological sections, are obtained allowing the visualization of the cells and the precise structure of the different layers that compose the tissue. The sensitivity of our device enables imaging the entire thickness of the cornea, even in edematous corneas more than 800 μm thick. Furthermore, we provide tomographic 3-D images of laser incisions inside the tissue at various depths without slicing the studied corneas. The effects of laser ablations can be observed, along various optical sections, directly in the bulk of the sample with high accuracy, providing information on the interface quality and also imaging tiny changes of the tissue structure. FF-OCT appears to be a powerful tool for subcellular imaging of the corneal structure and pathologies on the entire thickness of the tissue as well as interface quality and changes in the collagen structure due to laser incisions on ex vivo human cornea.
Aix-Marseille Université, Ecole Centrale Marseille, CNRS, Institut Fresnel, Domaine Universitaire de Saint-Jérôme, 13397 Marseille Cedex 20, France.
Gaël
G
Latour
Gaëlle
G
Georges
Laure Siozade
LS
Lamoine
Carole
C
Deumié
John
J
Conrath
Louis
L
Hoffart
D003315:Cornea / Q000033:anatomy & histology* / Q000601:surgery*; D015715:Corneal Edema / Q000473:pathology; D048988:Corneal Surgery, Laser; D016039:Corneal Transplantation; D005583:Fourier Analysis; D006801:Humans; D021621:Imaging, Three-Dimensional; D066298:In Vitro Techniques; D055620:Optical Phenomena; D041623:Tomography, Optical Coherence / Q000295:instrumentation / Q000379:methods* / Q000706:statistics & numerical data