Studies on solvatochromic properties of aminophenylstyryl-quinolinum dye, LDS 798, and its application in studying submicron lipid based structure.
The styryl group of dyes has been used in cellular studies for over 20 years because of their solvatochromic and/or electrochromic properties. Here we report characterization of solubility and solvatochromic properties of a near infra-red styryl dye, styryl 11 or LDS 798. We have extended our studies to small unilamellar vesicles and lipid based nanoparticles and found that solvatochromic properties of this dye used in tandem with fluorescence correlation spectroscopy can be used to efficiently determine the diffusion coefficient and hence the size of the submicron lipid based particles. This technique has the potential to provide essential information about liposomal and vesicular structures and their movement in vitro and in situ.
Center for Commercialization of Fluorescence Technologies, University of North Texas Health Science Center, Fort Worth, TX 76107, United States. psarkar@live.unthsc.edu
Pabak
P
Sarkar
Rafal
R
Luchowski
Sangram
S
Raut
Nirupama
N
Sabnis
Alan
A
Remaley
Andras G
AG
Lacko
Sanjay
S
Thamake
Zygmunt
Z
Gryczynski
Ignacy
I
Gryczynski
D000814:Aniline Compounds / Q000737:chemistry*; D004396:Coloring Agents / Q000737:chemistry*; D004058:Diffusion; D053758:Nanoparticles / Q000737:chemistry; D010316:Particle Size; D011806:Quinolinium Compounds / Q000737:chemistry*; D012995:Solubility; D012997:Solvents / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D053835:Unilamellar Liposomes / Q000737:chemistry
C556662:1-ethyl-4-(4-(p-dimethylaminophenyl)-1,3-butadienyl)quinolinium; D000814:Aniline Compounds; D004396:Coloring Agents; D011806:Quinolinium Compounds; D012997:Solvents; D053835:Unilamellar Liposomes