[Quantitative determination of forsythiaside in Forsythia suspensa].
OBJECTIVE
To develop a quantitative determination of Forsythia suspensa and to provide scientific basis for its quality control.


METHODS
By HPLC, the separation was performed on a Zorebax Eclipse XDB-C18 column (4.6 mm x 250 mm, 5 microm) at 25 degrees C using a isocratic elution of acetonitrile-water (containing 0.4% glacial acetic acid, 15:85). The detection wavelength was 330 nm.


RESULTS
The calibration curve showed a good linearity (r = 0.9998) within test range of 0.202-1.515 microg. And the average recovery was 98.54% with RSD 1.1%. The content range of forsythoside A in 11 batches of Forsythiae Fructus was 0.200%-1.681%.


CONCLUSIONS
The developed method is simple, accurate, specific, and with good repeatability. It is suitable for quality evaluation of Forsythiae Fructus.
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Bohou
B
Xia
Jingjing
J
Zhu
Zhimin
Z
Wang
Limei
L
Lin
Huimin
H
Gao
D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D031657:Forsythia / Q000737:chemistry*; D006027:Glycosides / Q000032:analysis*; D015203:Reproducibility of Results
D004365:Drugs, Chinese Herbal; D006027:Glycosides; C058049:forsythiaside