Evidence for the Presence of a Low Mr GTP-binding Protein, ram p25, in Human Platelet Membranes.
The ram gene was isolated from a rat megakaryocyte cDNA library and encodes a GTP-binding protein with a Mr of 25 000. In order to clarify the presence of the ram protein (ram p25) in human platelets, we tried to purify ram p25 from the sodium cholate extract of human platelet membranes by a combination of DEAE-Sephacel, Sephacryl S300HR, hydroxyapatite HCA-100S and DEAE-Toyopearl 650(S) column chromatographies. In the course of the purification, a specific antibody raised against a synthetic COOH-terminal peptide of ram p25 was used. In conclusion, ram p25 was partially purified and was observed to be present in membrane fractions of human platelets.
Department of Biochemistry, Gifu University School of Medicine, Tsukasamachi-40, Gifu, 500, Japan.
K
K
Nagata
Y
Y
Okano
T
T
Suzuki
Y
Y
Nozawa