[Experimental study on antitumor effects of extracts from Actinidia argutaor].
OBJECTIVE
To observe the inhibitory effect of extract from Actinidia argutaor on in vivo and in vitro carcinomata, and explore its mechanism.


METHODS
The in vivo S180 and H22 model were used to observe the effect of A. argutaor on inhibitory rate of carcinomata, organ relative weight of spleen and thymus gland and the release of tumor necrosis factor. A549 cells were exposed to extract from A. argutaor with different concentrations for 24, 48, 72 hours. MTT assay was used to evaluate the inhibiting effects of extract from A. argutaor on the proliferation of the cells. Flow cytometry was applied to detect cell cycle.


RESULTS
The inhibitory effects of the extracts on in vivo and in vitro carcinomata were observed, the inhibitory rate for S180 and H22 line was 33.32% and 34.62% respectively. The extracts could inhibit the proliferation of A549 cells during G0-G1 period and significantly decrease the cell ratio of S stage.


CONCLUSIONS
The extracts from A. argutaor showed a good antineoplastic activity.
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Lan
L
Wang
Chen
C
Kang
Weipeng
W
Yang
Manling
M
Li
Yanli
Y
Wang
Yiwei
Y
Wang
Luqi
L
Hang
Rixin
R
Liang
Qing
Q
Yang
Xiaojie
X
Yin
D029042:Actinidia / Q000737:chemistry*; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR
D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal