An increased risk of differentiated thyroid carcinoma in Iran with the 677C→T homozygous polymorphism in the MTHFR Gene.
BACKGROUND
Methylenetetrahydrofolate reductase (MTHFR) involves in folic acid metabolism which influences DNA methylation. A single nucleotide polymorphism (SNP) called 677C→T in MTHFR gene causes producing a thermolabile enzyme with reducing function and eventually defects DNA methylation. To determine association between germ-line polymorphism in MTHFR gene with differentiated thyroid carcinoma (DTC), this preliminary study was designed.


METHODS
This was a case-control study of 154 DTC patients and 198 cancer free individuals. Genotyping was performed by a multiplex PCR method and the frequencies of the 677C→T SNP in cases and controls were compared. The risk estimation was done by multivariate logistic regression analysis.


RESULTS
Compared to CC genotype, an increased risk of DTC for the 677C→T homozygous genotype was demonstrated (odds ratio [OR]: 2.08, 95% confidence interval [CI]: 0.82-5.25). Also, multivariate analysis demonstrated an increased risk of DTC in recessive fashion (TT vs. CC or CT) (OR: 2.38, 95% CI: 0.97-5.82).


CONCLUSIONS
The MTHFR 677C→T homozygous variant allele may be associated with increased risk of DTC.
Pasteur Institute of Iran, Biochemistry Department, Tehran, Iran. fard-esfahani@pasteur.ac.ir
Pezhman
P
Fard-Esfahani
Armaghan
A
Fard-Esfahani
Parinaz
P
Saidi
Shima
S
Fayaz
Reyhaneh
R
Mohabati
Mina
M
Majdi
D016022:Case-Control Studies; D002454:Cell Differentiation; D005260:Female; D005838:Genotype; D006720:Homozygote; D006801:Humans; D007492:Iran / Q000453:epidemiology; D008297:Male; D042965:Methylenetetrahydrofolate Reductase (NADPH2) / Q000235:genetics*; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D012307:Risk Factors; D013964:Thyroid Neoplasms / Q000453:epidemiology / Q000235:genetics*
D042965:Methylenetetrahydrofolate Reductase (NADPH2)