[Investigation of therapeutic mechanism of Weiweifang on experimental gastric ulcer in rats viewing from metabonomics].
OBJECTIVE
To investigate the therapeutic mechanism of Weiweifang (WWF, a Chinese herbal preparation) on gastric ulcer in rats viewing from metabonomics.


METHODS
Wistar rats were made to gastric model by acetic acid cauterization and randomized into the model group, the spontaneously healing group and the three WWF treatment groups, and a group of normal rats was set for control. Metabolic spectra of gastric mucosa extraction of rats were acquired with gas chromatography-mass spectrometry (GC-MS) technique. After being pre-processing, data were subjected to partial least squares discriminant analysis (PLS-DA) to discover the biomarkers in rats of the normal group and the model group. The therapeutic effect of WWF on experimental gastric ulcer was assessed by principal component analyses (PCA), and its action of mechanism was explained viewing from the changes of biomarkers.


RESULTS
Spectra of biomarkers, including organic acids, fatty acids, amino acids, etc. in model rats were statistically different to those in normal rats, which demonstrated that the energy and substance metabolisms were disordered in rats with gastric ulcer. WWF could cure gastric ulcer effectively by way of regulating the metabolism of gastric mucosa.


CONCLUSIONS
The therapeutic mechanism of WWF on experimental gastric ulcer in rats is revealed integrally by metabonomics in this study, displaying prominently the characteristics of Chinese medicine multiple targets comprehensive therapy.
School of Traditional Chinese Medicine, Southern Medical University, Guangzhou.
Shu-Ling
SL
Peng
Xiao-Wei
XW
Liu
Zhen-Rui
ZR
Zhang
D019342:Acetic Acid; D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D005227:Fatty Acids / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D005753:Gastric Mucosa / Q000378:metabolism; D008297:Male; D055432:Metabolomics / Q000379:methods*; D008517:Phytotherapy; D051381:Rats; D017208:Rats, Wistar; D013276:Stomach Ulcer / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism
D000596:Amino Acids; D004365:Drugs, Chinese Herbal; D005227:Fatty Acids; D019342:Acetic Acid