Staphylococcal IgM enzyme-linked immunosorbent assay for diagnosis of periprosthetic joint infections.
Delayed orthopedic joint prosthesis infections (DOJP-Is) due to staphylococci frequently result in prosthetic revision. Specific and noninvasive diagnostic tests are unavailable, and DOJP-Is are commonly diagnosed at advanced stages of disease. An enzyme-linked immunosorbent assay (ELISA) was developed to detect serum antibodies against staphylococcal slime polysaccharide antigens. Using a cutoff of 0.35 ELISA units, the test showed a specificity of 95.1% (95% confidence interval [CI], 85.4 to 98.7%) and a sensitivity of 89.7% (71.5 to 97.3%) on a sample of 90 individuals.
Department of Public Health and Infectious Diseases, Sapienza University, P. le Aldo Moro 5, 00185 Rome, Italy.
Marco
M
Artini
Carlo
C
Romanò
Lamberto
L
Manzoli
Gian Luca
GL
Scoarughi
Rosanna
R
Papa
Enzo
E
Meani
Lorenzo
L
Drago
Laura
L
Selan
D000907:Antibodies, Bacterial / Q000097:blood*; D001168:Arthritis / Q000382:microbiology*; D001431:Bacteriological Techniques / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood*; D016459:Prosthesis-Related Infections / Q000175:diagnosis* / Q000382:microbiology; D012680:Sensitivity and Specificity; D013203:Staphylococcal Infections / Q000175:diagnosis* / Q000382:microbiology; D013210:Staphylococcus / Q000302:isolation & purification*
D000907:Antibodies, Bacterial; D007075:Immunoglobulin M