Measurement of myeloid cell immune suppressive activity.
This unit presents simple methods to assess the immunosuppressive properties of immunoregulatory cells of myeloid origin, such as myeloid-derived suppressor cells (MDSCs), both in vitro and in vivo. These methods are general and could be adapted to test the impact of different suppressive populations on T cell activation, proliferation, and cytotoxic activity; moreover they could be useful to assess the influence exerted on immune suppressive pathways by genetic modifications, chemical inhibitors, and drugs.
Istituto Oncologico Veneto, IRCCS, Padua, Italy.
Luigi
L
Dolcetti
Elisa
E
Peranzoni
Vincenzo
V
Bronte
D000818:Animals; D001671:Biochemistry / Q000379:methods; D049109:Cell Proliferation; D003602:Cytotoxicity, Immunologic; D006801:Humans; D007108:Immune Tolerance; D007158:Immunologic Techniques; D007165:Immunosuppression Therapy / Q000379:methods; D008213:Lymphocyte Activation; D008967:Molecular Biology / Q000379:methods; D022423:Myeloid Cells / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology