TLR 9 activation in dendritic cells enhances salmonella killing and antigen presentation via involvement of the reactive oxygen species.
Synthetic CpG containing oligodeoxynucleotide Toll like receptor-9 agonist (CpG DNA) activates innate immunity and can stimulate antigen presentation against numerous intracellular pathogens. It was observed that Salmonella Typhimurium growth can be inhibited by the CpG DNA treatment in the murine dendritic cells. This inhibitory effect was mediated by an increased reactive oxygen species production. In addition, it was noted that CpG DNA treatment of dendritic cells during Salmonella infection leads to an increased antigen presentation. Further this increased antigen presentation was dependent on the enhanced reactive oxygen species production elicited by Toll like receptor-9 activation. With the help of an exogenous antigen it was shown that Salmonella antigen could also be cross-presented in a better way by CpG induction. These data collectively indicate that CpG DNA enhance the ability of murine dendritic cells to contain the growth of virulent Salmonella through reactive oxygen species dependent killing.
Center for Infectious Disease Research and Biosafety Laboratories, Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, India.
Amit
A
Lahiri
Ayan
A
Lahiri
Priyanka
P
Das
Janakiraman
J
Vani
M S
MS
Shaila
Dipshikha
D
Chakravortty
D000942:Antigens, Bacterial / Q000276:immunology*; D018899:CpG Islands; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D017382:Reactive Oxygen Species / Q000378:metabolism*; D012475:Salmonella / Q000276:immunology*; D051217:Toll-Like Receptor 9 / Q000378:metabolism*
D000942:Antigens, Bacterial; D017382:Reactive Oxygen Species; C495371:TLR9 protein, human; D051217:Toll-Like Receptor 9