Platelet receptor polymorphisms do not influence Staphylococcus aureus-platelet interactions or infective endocarditis.
Cardiac vegetations result from bacterium-platelet adherence, activation and aggregation, and are associated with increased morbidity and mortality in infective endocarditis. The GPIIb/IIIa and FcγRIIa platelet receptors play a central role in platelet adhesion, activation and aggregation induced by endocarditis pathogens such as Staphylococcus aureus, but the influence of known polymorphisms of these receptors on the pathogenesis of infective endocarditis is unknown. We determined the GPIIIa platelet antigen Pl(A1/A2) and FcγRIIa H131R genotype of healthy volunteers (n = 160) and patients with infective endocarditis (n = 40), and investigated the influence of these polymorphisms on clinical outcome in infective endocarditis and S. aureus-platelet interactions in vitro. Platelet receptor genotype did not correlate with development of infective endocarditis, vegetation characteristics on echocardiogram or the composite clinical end-point of embolism, heart failure, need for surgery or mortality (P > 0.05 for all), even though patients with the GPIIIa Pl(A1/A1) genotype had increased in vivo platelet activation (P = 0.001). Furthermore, neither GPIIIa Pl(A1/A2) nor FcγRIIa H131R genotype influenced S. aureus-induced platelet adhesion, activation or aggregation in vitro (P > 0.05). Taken together, our data suggest that the GPIIIa and FcγRIIa platelet receptor polymorphisms do not influence S. aureus-platelet interactions in vitro or the clinical course of infective endocarditis.
Center for Infectious Diseases and The Roslin Institute, Royal (Dick) School of Veterinary Studies, University of Edinburgh, Chancellor's Building, Edinburgh EH164SB, United Kingdom.
Shruti
S
Daga
James G
JG
Shepherd
J Garreth S
JG
Callaghan
Rachel K Y
RK
Hung
Dana K
DK
Dawson
Gareth J
GJ
Padfield
Shi Y
SY
Hey
Robyn A
RA
Cartwright
David E
DE
Newby
J Ross
JR
Fitzgerald
D000328:Adult; D000368:Aged; D001792:Blood Platelets / Q000382:microbiology* / Q000502:physiology; D004696:Endocarditis / Q000097:blood* / Q000235:genetics / Q000382:microbiology; D005260:Female; D054884:Host-Pathogen Interactions; D006801:Humans; D039661:Integrin beta3 / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D015539:Platelet Activation; D011110:Polymorphism, Genetic; D017452:Receptors, IgG / Q000235:genetics*; D013203:Staphylococcal Infections / Q000097:blood* / Q000235:genetics / Q000382:microbiology; D013211:Staphylococcus aureus / Q000502:physiology*; D018709:Statistics, Nonparametric
C080568:Fc gamma receptor IIA; D039661:Integrin beta3; D017452:Receptors, IgG