[Investigation on quality standard of Franchet groundcherry].
OBJECTIVE
To develop identification and assay methods of Franchet groundcherry.


METHODS
TLC method was used to identify of physalin L in the sample using high performance silica gel G plate and a mixture of chloroform-acetone-methanol (25:1:1) as a developing solvent. In the chromatogram, physalin L showed a distinct fluorescence spot under UV 365 nm with good separation. In the HPLC method, luteoloside was separated on a Venusil XBP C18 (4.6 mm x 250 mm, 5 microm) column with acetonitrile-0. 2% phosphoric acid (20:80) as the mobile phase with flow rate of 1 mL x min(-1). The detection wavelength was set at 350 nm.


RESULTS
For the HPLC quantitation method, the calibration curve of luteoloside displayed ideal linearity over the range of 0.50-249.40 mg x L(-1) with the regression equation of Y = 55,313X + 3.1641 (r = 1.000). The average recovery of luteoloside was 98.79% with a RSD of 1.1%. And the intra-day and inter-day precisions were less than 2%.


CONCLUSIONS
The TLC identification and HPLC determination were sensitive, reliable and repeatable and can be applied for the quality evaluation and assessment of Franchet Groundcherry Calyx.
Shanghai Research and Development Centre for Standardization of Chinese Medicines, Shanghai 201203, China. chengxuemei1963@163.com
Xuemei
X
Cheng
Chuhang
C
Zhang
Guixin
G
Chou
Zhengtao
Z
Wang
D029402:Bignoniaceae / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D004365:Drugs, Chinese Herbal / Q000032:analysis; D009844:Oligosaccharides / Q000032:analysis; D015203:Reproducibility of Results
D004365:Drugs, Chinese Herbal; D009844:Oligosaccharides