BECLIN1 from Arabidopsis thaliana under the generic control of regulated expression systems, a strategy for developing male sterile plants.
Induction of male sterility followed by successful outcrossing is a prerequisite for hybrid seed production. In this article, we have identified a novel use of the BECLIN 1 gene of Arabidopsis, in inducing male sterility in plants, when expressed in the anther tapetum of tobacco. We also report a stringently regulated and high-level expression of the desired gene in tapetum by using a two-component transcription regulation system. The tapetum-specific, two-component transcription system utilizes the TGTA-TBPm³ complementation principle that has been demonstrated by us earlier. We also report a glucocorticoid-dependent expression of AtBECLIN 1 in tapetum, thereby developing glucocorticoid-inducible male sterility in plants.
Plant Molecular Biology and Genetic Engineering Division, National Botanical Research Institute, Council of Scientific and Industrial Research, Rana Pratap Marg, Lucknow, India.
Sudhir P
SP
Singh
Tripti
T
Pandey
Rakesh
R
Srivastava
Praveen C
PC
Verma
Pradhyumna K
PK
Singh
Rakesh
R
Tuli
Samir V
SV
Sawant
D033942:Adaptor Proteins, Vesicular Transport / Q000235:genetics / Q000378:metabolism*; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D000071186:Beclin-1; D018506:Gene Expression Regulation, Plant / Q000235:genetics / Q000502:physiology; D051479:Plant Infertility / Q000235:genetics / Q000502:physiology; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism*
D033942:Adaptor Proteins, Vesicular Transport; D029681:Arabidopsis Proteins; D000071186:Beclin-1; C519561:beclin 1 protein, Arabidopsis