Residue-specific incorporation of non-canonical amino acids into proteins: recent developments and applications.
Residue-specific incorporation of non-canonical amino acids into proteins allows facile alteration and enhancement of protein properties. In this review, we describe recent technical developments and applications of residue-specific incorporation to problems ranging from elucidation of biochemical mechanisms to engineering of protein-based biomaterials. We hope to inform the reader of the ease and broad utility of residue-specific non-canonical amino acid incorporation with the goal of inspiring investigators outside the field to consider applying this tool to their own research.
Division of Chemistry and Chemical Engineering, Joseph J. Jacobs Institute for Molecular Engineering for Medicine, California Institute of Technology, Pasadena, CA 91125, USA.
Jeremiah A
JA
Johnson
Ying Y
YY
Lu
James A
JA
Van Deventer
David A
DA
Tirrell
D000596:Amino Acids / Q000737:chemistry* / Q000378:metabolism; D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism; D000818:Animals; D006801:Humans; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism
D000596:Amino Acids; D011506:Proteins; D000604:Amino Acyl-tRNA Synthetases