Effects of some drugs on human erythrocyte glucose 6-phosphate dehydrogenase: an in vitro study.
Inhibitory effects of some drugs on glucose 6-phosphate dehydrogenase from the erythrocytes of human have been investigated. For this purpose, at the beginning, erythrocyte glucose 6-phosphate dehydrogenase was purified 2256 times in a yield of 44.22% by using ammonium sulphate precipitation and 2', 5'-ADP Sepharose 4B affinity gel. Temperature of +4°C was maintained during the purification process. Enzyme activity was determined with the Beutler method by using a spectrophotometer at 340 nm. This method was utilized for all kinetic studies. Ketotifen, dacarbazine, thiocolchicoside, meloxicam, methotrexate, furosemide, olanzapine, methylprednizolone acetate, paricalcitol, ritodrine hydrochloride, and gadobenate-dimeglumine were used as drugs. All the drugs indicated the inhibitory effects on the enzyme. Ki constants for glucose 6-phosphate dehydrogenase were found by means of Lineweaver-Burk graphs. While methylprednizolone acetate showed competitive inhibition, the others displayed non-competitive inhibition. In addition, IC(50) values of the drugs were determined by plotting Activity% vs [I].
Ataturk University, Science Faculty, Department of Chemistry, Erzurum, Turkey.
Ebru
E
Akkemik
Harun
H
Budak
Mehmet
M
Ciftci
D000328:Adult; D001667:Binding, Competitive; D004791:Enzyme Inhibitors / Q000009:adverse effects / Q000494:pharmacology*; D004912:Erythrocytes / Q000201:enzymology*; D005954:Glucosephosphate Dehydrogenase / Q000037:antagonists & inhibitors* / Q000302:isolation & purification / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D008297:Male; D010427:Pentose Phosphate Pathway / Q000187:drug effects; D013457:Sulfur Compounds / Q000009:adverse effects / Q000494:pharmacology
D004791:Enzyme Inhibitors; D013457:Sulfur Compounds; D005954:Glucosephosphate Dehydrogenase