Simple and biocompatible micropatterning of multiple cell types on a polymer substrate by using ion implantation.
A noncytotoxic procedure for the spatial organization of multiple cell types remains as a major challenge in tissue engineering. In this study, a simple and biocompatible micropatterning method of multiple cell types on a polymer surface is developed by using ion implantation. The cell-resistant Pluronic surface can be converted into a cell-adhesive one by ion implantation. In addition, cells show different behaviors on the ion-implanted Pluronic surface. Thus this process enables the micropatterning of two different cell types on a polymer substrate. The micropatterns of the Pluronic were formed on a polystyrene surface. Primary cells adhered to the spaces of the bare polystyrene regions separated by the implanted Pluronic patterns. Secondary cells then adhered onto the implanted Pluronic patterns, resulting in micropatterns of two different cells on the polystyrene surface.
Radiation Research Division for Industry and Environment, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup-si, Jeollabuk-do 580-185, Republic of Korea.
In-Tae
IT
Hwang
Chan-Hee
CH
Jung
Jae-Hak
JH
Choi
Young-Chang
YC
Nho
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002448:Cell Adhesion; D002621:Chemistry / Q000379:methods*; D006801:Humans; D007477:Ions; D008422:Materials Testing; D051379:Mice; D008853:Microscopy / Q000379:methods; D041681:NIH 3T3 Cells; D011108:Polymers / Q000737:chemistry*; D011137:Polystyrenes / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*
D001672:Biocompatible Materials; D007477:Ions; D011108:Polymers; D011137:Polystyrenes