A modified rapid enzymatic microtiter plate assay for the quantification of intracellular γ-aminobutyric acid and succinate semialdehyde in bacterial cells.
The GABase assay is widely used to rapidly and accurately quantify levels of extracellular γ-aminobutyric acid (GABA). Here we demonstrate a modification of this assay that enables quantification of intracellular GABA in bacterial cells. Cells are lysed by boiling and ethanolamine-O-sulphate, a GABA transaminase inhibitor is used to distinguish between GABA and succinate semialdehyde.
Bacterial Stress Response Group, Microbiology, School of Natural Sciences, NUI Galway, Galway, Ireland.
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O'Byrne
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Feehily
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Ham
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Karatzas
D000613:Aminobutyrates / Q000032:analysis*; D001419:Bacteria / Q000737:chemistry*; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D005680:gamma-Aminobutyric Acid / Q000031:analogs & derivatives* / Q000032:analysis
D000613:Aminobutyrates; D005680:gamma-Aminobutyric Acid; C009338:succinic semialdehyde