Obesity, age, and oxidative stress in middle-aged and older women.
Recent evidence suggests that urinary F(2)-isoprostanes (F(2)-IsoPs) are more accurate markers of oxidative stress than other available biomarkers. Most previous studies used unmetabolized F(2)-IsoPs as a biomarker. Few previous studies measured 15-F(2t)-IsoP-M, a metabolite of one of the most common F(2)-IsoPs, 15-F(2t)-IsoP. Unlike 15-F(2t)-IsoP, 15-F(2t)-IsoP-M is not subject to autoxidation and renal production. To our knowledge, no study has compared the associations of age and body mass index (BMI) with F(2)-IsoPs to those with 15-F(2t)-IsoP-M. Urinary levels of F(2)-IsoPs and 15-F(2t)-IsoP-M were measured using gas chromatography-mass spectrometry for 845 healthy women aged 40-70 years. Both F(2)-IsoPs and 15-F(2t)-IsoP-M were elevated among smokers. The level of 15-F(2t)-IsoP-M increased with age, particularly after menopause, and with BMI. In contrast, F(2)-IsoPs decreased with age, regardless of menopausal status, and was not related to BMI. The association of 15-F(2t)-IsoP-M with age or menopausal status did not differ by BMI category, and the association with BMI was also independent of age or menopausal status. 15-F(2t)-IsoP-M appears to be a valuable biomarker of oxidative stress in age- and obesity-related diseases.
Department of Medicine, Vanderbilt Epidemiology Center, Vanderbilt-Ingram Cancer Center, Vanderbilt University School of Medicine, 2525 West End Ave., Nashville, TN 37203-1738, USA.
Tsogzolmaa
T
Dorjgochoo
Yu-Tang
YT
Gao
Wong-Ho
WH
Chow
Xiao-Ou
XO
Shu
Gong
G
Yang
Quiyin
Q
Cai
Nathaniel
N
Rothman
Hui
H
Cai
Honglan
H
Li
Xinqing
X
Deng
Martha J
MJ
Shrubsole
Harvey
H
Murff
Ginger
G
Milne
Wei
W
Zheng
Qi
Q
Dai
D000328:Adult; D000367:Age Factors; D000368:Aged; D044466:Asian People; D015415:Biomarkers / Q000652:urine*; D015992:Body Mass Index; D003430:Cross-Sectional Studies; D028441:F2-Isoprostanes / Q000652:urine*; D005260:Female; D006801:Humans; D008593:Menopause; D008875:Middle Aged; D009765:Obesity / Q000503:physiopathology / Q000652:urine*; D018384:Oxidative Stress / Q000502:physiology*; D016387:Women's Health
D015415:Biomarkers; D028441:F2-Isoprostanes