The physiological response of larval Chironomus riparius (Meigen) to abrupt brackish water exposure.
The physiological response of larval Chironomus riparius was examined following direct transfer from freshwater (FW) to brackish water (BW; 20% seawater). Endpoints of hydromineral status (hemolymph Na⁺, Cl⁻, and K⁺ levels, hemolymph pH, body water content, and whole body Na⁺/K⁺-ATPase and V-type H⁺-ATPase activity) were examined 1, 3, 5, 12 and 24 h following BW transfer. Larvae transferred from FW to FW served as a control. Hemolymph Na⁺ and Cl⁻ levels increased following BW transfer. Hemolymph pH was initially regulated, but significantly decreased after 24 h in BW. Changes in hemolymph ions were not caused by osmotic loss of water from the hemolymph, since larvae tightly regulated total body moisture content. Furthermore, salinity did not affect hemolymph K⁺. When larvae were transferred to BW, Na⁺/K⁺-ATPase (NKA) activity did not significantly alter relative to FW control animals. In contrast, V-type H⁺-ATPase (VA) activity in C. riparius significantly decreased in BW. In FW-reared C. riparius, whole body NKA and VA activities were equivalent. However, in the isolated gut with intact Malpighian tubules of FW-reared C. riparius, VA activity was significantly greater than whole body while NKA activity was equivalent. This suggested that gut and/or Malpighian tubule VA activity contributes significantly to whole body VA activity and that a decline in whole body VA activity in BW may be closely linked to alterations in the physiology of gut and Malpighian tubule tissue. Taken together, data indicate that VA is important for ion uptake in FW and that the NKA does not play a major role in regulating ion homeostasis when larvae are acutely exposed to BW.
Department of Biology, York University, 4700 Keele Street, Toronto, ON M3J 1P3, Canada.
Sima
S
Jonusaite
Scott P
SP
Kelly
Andrew
A
Donini
D000222:Adaptation, Physiological; D000818:Animals; D001834:Body Water; D002683:Chironomidae / Q000502:physiology*; D002712:Chlorides / Q000378:metabolism; D017753:Ecosystem; D005618:Fresh Water; D041981:Gastrointestinal Tract / Q000378:metabolism; D006458:Hemolymph / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D017136:Ion Transport; D007814:Larva / Q000502:physiology; D008317:Malpighian Tubules / Q000378:metabolism; D011188:Potassium / Q000378:metabolism; D017494:Proton Pumps / Q000378:metabolism; D054712:Salinity; D012964:Sodium / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D025262:Vacuolar Proton-Translocating ATPases / Q000378:metabolism
D002712:Chlorides; D017494:Proton Pumps; D012964:Sodium; D025262:Vacuolar Proton-Translocating ATPases; D000254:Sodium-Potassium-Exchanging ATPase; D011188:Potassium