DNA extraction from freeze-dried plant tissue with CTAB in a 96-well format.
This protocol is a modified version of DNA isolation using cetyltrimethylammonium bromide (CTAB) and 96-well plates. It is high-throughput, which facilitates the analysis of large mapping populations. The DNA yield is adequate for at least 100-500 polymerase chain reaction (PCR) procedures.
An-Ping
AP
Hsia
Hsin D
HD
Chen
Kazuhiro
K
Ohtsu
Patrick S
PS
Schnable
D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000737:chemistry* / Q000378:metabolism*; D018744:DNA, Plant / Q000235:genetics / Q000302:isolation & purification*; D005612:Freeze Drying; D008967:Molecular Biology / Q000379:methods*; D012997:Solvents
D002593:Cetrimonium Compounds; D018744:DNA, Plant; D012997:Solvents; D000077286:Cetrimonium