Cloning and characterization of Kluyveromyces marxianus Hog1 gene.
The mitogen-activated protein kinase Hog1 gene (Kmhog1) was isolated from Kluyveromyces marxianus strain NBRC 1777 by degenerate PCR and genome walking, and then disrupted to construct a mutant strain hog1∆. The mutant was now more sensitive to acetic acid and its growth was nearly completely inhibited by 0.5 M NaCl (97%) and 10 mM H(2)O(2) (93%) as compared with the wild-type cells. However, neither strain grew at 47°C. Kmhog1 may thus be required for adaptation to acetic acid, osmotic, and oxidative stress but is not involved in thermotolerance.
State Key Laboratory of Bioreactor Engineering, East China University of Science & Technology, 130 Meilong Road, Shanghai, 200237, People's Republic of China.
Jiangchao
J
Qian
Xiulin
X
Qin
Qin
Q
Yin
Ju
J
Chu
Yonghong
Y
Wang
D019342:Acetic Acid / Q000494:pharmacology; D016386:Chromosome Walking; D003001:Cloning, Molecular; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007716:Kluyveromyces / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D016133:Polymerase Chain Reaction
D005656:Fungal Proteins; D006861:Hydrogen Peroxide; D019342:Acetic Acid