MUC16 as a sensitive and specific marker for epithelial downgrowth.
OBJECTIVE
To compare immunohistochemical results of cytokeratin AE1/AE3, the traditional favored marker, with MUC16 and cytokeratin 19 for diagnostic sensitivity and specificity in epithelial downgrowth and control corneas.


METHODS
Immunohistochemical analysis was performed in 5 cases of epithelial downgrowth and 5 control specimens for MUC16, cytokeratin AE1/AE3, and cytokeratin 19 using the immunoperoxidase method. The mean percentages of reactive cells on the epithelium and endothelium were compared for each antibody using the Wilcoxon rank sum test. The sensitivity and specificity for each marker were compared.


RESULTS
All 3 antibodies showed high sensitivity (100%) in identifying epithelial downgrowth. However, the specificity was greatest for MUC 16 (100%) compared with cytokeratin 19 (80%) and cytokeratin AE1/AE3 (0%). None of the endothelial cells in any case showed reactivity to anti-MUC16 compared with anti-cytokeratin AE1/AE3 (mean [SD], 0.0% [0.0%] vs 17.4% [10.4%]; P = .008). Cytokeratin 19 was positive in every case of epithelial downgrowth but showed focal staining of the endothelium (3.4% of cells) in 1 control.


CONCLUSIONS
Antibodies for MUC16, cytokeratin AE1/AE3, and cytokeratin 19 are equally sensitive for downgrowth. However, anti-MUC16 showed superior specificity compared with anti-cytokeratin 19 or anti-cytokeratin AE1/AE3 in this study.
Department of Ophthalmology, Jules Stein Eye Institute, University of California at Los Angeles School of Medicine, Los Angeles, CA 90095-7000, USA.
Vicky C
VC
Pai
Ben J
BJ
Glasgow
D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000378:metabolism*; D018394:CA-125 Antigen / Q000378:metabolism*; D003316:Corneal Diseases / Q000175:diagnosis* / Q000378:metabolism*; D019573:Epithelium, Corneal / Q000473:pathology*; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D053556:Keratin-1 / Q000378:metabolism; D053539:Keratin-19 / Q000378:metabolism; D053558:Keratin-3 / Q000378:metabolism; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism*; D008875:Middle Aged; D012680:Sensitivity and Specificity
D015415:Biomarkers; D018394:CA-125 Antigen; C491616:KRT1 protein, human; C479360:KRT3 protein, human; D053556:Keratin-1; D053539:Keratin-19; D053558:Keratin-3; C451759:MUC16 protein, human; D008565:Membrane Proteins