Agrobacterium tumefaciens-mediated transformation of the vegetative dikaryotic mycelium of the cultivated mushroom Flammulina velutipes.
Agrobacterium tumefaciens was used to transform the vegetative dikaryotic mycelium of Flammulina velutipes using a hygromycin B resistance gene as selectable marker. The gene coding for urogen III methyltransferase (cob) was introduced into F. velutipes dikaryotic cells. The resulting transformant cells generated a bright red fluorescence, indicating that cob is promising as a reporter gene in F. velutipes.
Division of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, Ueda, Nagano, Japan.
Tomomitsu
T
Okamoto
Masato
M
Yamada
Shuichi
S
Sekiya
Toru
T
Okuhara
Goro
G
Taguchi
Satoshi
S
Inatomi
Makoto
M
Shimosaka
D016960:Agrobacterium tumefaciens / Q000235:genetics*; D000871:Anthelmintics; D000900:Anti-Bacterial Agents; D004351:Drug Resistance / Q000235:genetics; D055393:Flammulina / Q000235:genetics*; D005453:Fluorescence; D017930:Genes, Reporter / Q000235:genetics*; D006921:Hygromycin B / Q000494:pharmacology; D008780:Methyltransferases / Q000235:genetics; D025282:Mycelium / Q000235:genetics*; D014161:Transduction, Genetic
D000871:Anthelmintics; D000900:Anti-Bacterial Agents; D006921:Hygromycin B; D008780:Methyltransferases