A multicomponent recognition and separation system established via fluorescent, magnetic, dualencoded multifunctional bioprobes.
Accurate and rapid recognition and separation of multiple types of biological targets such as molecules, cells, bacteria or viruses from complex sample mixtures is of great importance for a wide range of diagnostic and therapeutic strategies. To achieve this goal, a set of fluorescent, magnetic, dual-encoded multifunctional bioprobes has been constructed by co-embedding different-sized quantum dots and varying amounts of γ-Fe(2)O(3) magnetic nanoparticles into swollen poly(styrene/acrylamide) copolymer nanospheres. The dual-encoded bioprobes, which possessed different photoluminescent property and magnetic susceptibility, were proven to be capable of simultaneously recognizing and separating multiple components from a complex sample when three kinds of lectins were used as the targets. The lectins were separated with high efficiency and kept their bioactivity during the process. Compared to the conventional batchwise separation, this method does not require a large number of sequential reaction steps, which is economical of time and can be very reagent-saving. By combining the multiplexing capability of quantum dots with the superparamagnetic properties of iron oxide nanoparticles, this dual-encoded technique is expected to open new opportunities in high-throughput and multiplex bioassays, such as cell sorting, proteomical and genomical applications, drug screening etc.
Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, PR China.
Jun
J
Hu
Min
M
Xie
Cong-Ying
CY
Wen
Zhi-Ling
ZL
Zhang
Hai-Yan
HY
Xie
An-An
AA
Liu
Yong-Yong
YY
Chen
Shi-Ming
SM
Zhou
Dai-Wen
DW
Pang
D001672:Biocompatible Materials / Q000737:chemistry; D015374:Biosensing Techniques; D005290:Ferric Compounds / Q000737:chemistry; D005453:Fluorescence; D057166:High-Throughput Screening Assays / Q000379:methods*; D037102:Lectins / Q000302:isolation & purification; D008280:Magnetics; D008422:Materials Testing; D053768:Metal Nanoparticles / Q000737:chemistry; D054118:Nanospheres / Q000737:chemistry*; D045663:Quantum Dots
D001672:Biocompatible Materials; D005290:Ferric Compounds; D037102:Lectins; C000499:ferric oxide