Prevention of tumour cell dissemination in diagnostic needle procedures.
BACKGROUND
A side effect of diagnostic needle biopsies is the possibility to disseminate tumour cells into the needle track, which may cause concern in certain malignant tumour types.


METHODS
In order to prevent tumour cell dissemination we developed a technology that uses radiofrequency (RF) pulses to sterilise the needle track and denaturate tumour cells. To determine feasibility, we applied this technology to fine needle aspiration biopsy (FNAB) and used breast cancer as a model tumour. Routine FNAB was performed in 88 patients with adenocarcinoma and blood droplets passing the skin orifice were cytomorphologically analysed for the presence of tumour cells.


RESULTS
The analysis showed the presence of tumour cells in 65/88 cases (74%). When using an experimental anti-seeding device in a subset of patients viable tumour cells were found in 0/31 cases (P<0.001). In all 31 patients blood passing the skin orifice was sparse. No degrading effect on the cytological sample inside the needle was detected and pain caused by the RF pulses was comparable to that of the biopsy procedure itself.


CONCLUSIONS
The herein presented method has the potential to prevent the dissemination of viable tumour cells in the needle track and minimize bleeding without additional pain or degradation of the aspirate.
Department of Molecular Medicine and Surgery, Karolinska Institutet, Karolinska University Hospital, Solna (L1:00), 17176 Stockholm, Sweden.
H
H
Wiksell
K-U
KU
Schässburger
M
M
Janicijevic
K
K
Leifland
L
L
Löfgren
S
S
Rotstein
P-O
PO
Sandberg
C
C
Wadström
G
G
Auer
D001707:Biopsy, Needle / Q000009:adverse effects*; D006801:Humans; D009366:Neoplasm Seeding; D009369:Neoplasms / Q000473:pathology*