Stearoyl-CoA desaturase-1 is associated with insulin resistance in morbidly obese subjects.
Animal studies have revealed the association between stearoyl-CoA desaturase 1 (SCD1) and obesity and insulin resistance. However, only a few studies have been undertaken in humans. We studied SCD1 in visceral adipose tissue (VAT) and subcutaneous adipose tissue (SAT) from morbidly obese patients and their association with insulin resistance, sterol regulatory element binding protein-1 (SREBP-1) and ATPase p97, proteins involved in SCD1 synthesis and degradation. The insulin resistance was calculated in 40 morbidly obese patients and 11 overweight controls. Measurements were made of VAT and SAT SCD1, SREBP-1 and ATPase p97 mRNA expression and protein levels. VAT and SAT SCD1 mRNA expression levels in the morbidly obese patients were significantly lower than in the controls (P = 0.006), whereas SCD1 protein levels were significantly higher (P < 0.001). In the morbidly obese patients, the VAT SCD1 protein levels were decreased in patients with higher insulin resistance (P = 0.007). However, SAT SCD1 protein levels were increased in morbidly obese patients with higher insulin resistance (P < 0.05). Multiple linear regressions in the morbidly obese patients showed that the variable associated with the SCD1 protein levels in VAT was insulin resistance, and the variables associated with SCD1 protein levels in SAT were body mass index (BMI) and ATPase p97. In conclusion, these data suggest that the regulation of SCD1 is altered in individuals with morbid obesity and that the SCD1 protein has a different regulation in the two adipose tissues, as well as being closely linked to the degree of insulin resistance.
CIBER Diabetes and Associated Metabolic Disorders, ISCIII, Spain.
Sara
S
García-Serrano
Inmaculada
I
Moreno-Santos
Lourdes
L
Garrido-Sánchez
Carolina
C
Gutierrez-Repiso
Jose M
JM
García-Almeida
Juan
J
García-Arnés
Jose
J
Rivas-Marín
Jose L
JL
Gallego-Perales
Eva
E
García-Escobar
Gemma
G
Rojo-Martinez
Francisco
F
Tinahones
Federico
F
Soriguer
Manuel
M
Macias-Gonzalez
Eduardo
E
García-Fuentes
D000251:Adenosine Triphosphatases / Q000235:genetics / Q000378:metabolism; D000273:Adipose Tissue / Q000201:enzymology* / Q000378:metabolism; D000328:Adult; D000368:Aged; D000818:Animals; D015153:Blotting, Western; D005260:Female; D006801:Humans; D007333:Insulin Resistance; D050152:Intra-Abdominal Fat / Q000201:enzymology / Q000378:metabolism; D016014:Linear Models; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D009767:Obesity, Morbid / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013230:Stearoyl-CoA Desaturase / Q000235:genetics / Q000378:metabolism*; D051780:Sterol Regulatory Element Binding Protein 1 / Q000235:genetics / Q000378:metabolism; D050151:Subcutaneous Fat / Q000201:enzymology / Q000378:metabolism
D009687:Nuclear Proteins; D020033:Protein Isoforms; D012333:RNA, Messenger; C497387:SREBF1 protein, human; D051780:Sterol Regulatory Element Binding Protein 1; C523776:SCD1 protein, human; D013230:Stearoyl-CoA Desaturase; D000251:Adenosine Triphosphatases; C404942:p97 ATPase