Production of retroviral vectors: review.
Retroviral vectors are presently amongst the most widely used vectors in gene therapy clinical trials to target pathologies of different origins, such as cancers, genetic diseases or neurological disorders. This review provides an overview on the evolution of retroviral vector design and production for gene therapy applications, including state of the art developments in flexible producer cells and safe vectors. In addition, production and purification processes will be addressed, with a particular focus on the improvements undertaken to increase vector productivity and to reduce the rapid loss of infectivity, which presently represent the main challenges in retroviral vectors production for gene therapy.
Instituto de Tecnologia Química e Biológica-Universidade Nova de Lisboa/Instituto de Biologia Experimental e Tecnológica (ITQB-UNL/IBET), P-2781-901 Oeiras, Portugal.
Ana S
AS
Coroadinha
Leonor
L
Gama-Norton
Ana I
AI
Amaral
Hansjorg
H
Hauser
Paula M
PM
Alves
Pedro E
PE
Cruz
D000818:Animals; D015316:Genetic Therapy; D005822:Genetic Vectors / Q000096:biosynthesis* / Q000302:isolation & purification*; D006801:Humans; D012190:Retroviridae / Q000235:genetics* / Q000254:growth & development