Biosensors for efficient diagnosis of leishmaniasis: innovations in bioanalytics for a neglected disease.
The need for reliable, fast diagnostics is closely linked to the need for safe, effective treatment of the so-called "neglected" diseases. The list of diseases with no field-adapted diagnostic tools includes leishmaniasis, shigella, typhoid, and bacterial meningitis. Leishmaniasis, in particular, is a parasitic disease caused by Leishmania spp. transmitted by infected phlebotomine sandfly, which remains a public health concern in developing countries with ca. 12 million people infected and 350 million at risk of infection. Despite several attempts, methods for diagnosis are still noneffective, especially with regard to specificity due to false positives with Chagas' disease caused by Trypanosoma cruzi . Accepted golden standards for detecting leishmaniasis involve isolation of parasites either microscopically, or by culture, and in both methods specimens are obtained by invasive means. Here, we show that efficient distinction between cutaneous leishmaniasis and Chagas' disease can be obtained with a low-cost biosensor system made with nanostructured films containing specific Leishmania amazonensis and T. cruzi antigens and employing impedance spectroscopy as the detection method. This unprecedented selectivity was afforded by antigen-antibody molecular recognition processes inherent in the detection with the immobilized antigens, and by statistically correlating the electrical impedance data, which allowed distinction between real samples that tested positive for Chagas' disease and leishmaniasis. Distinction could be made of blood serum samples containing 10(-5) mg/mL of the antibody solution in a few minutes. The methods used here are generic and can be extended to any type of biosensor, which is important for an effective diagnosis of many other diseases.
Instituto de Física de São Carlos, USP, CP 369, 13560-970 São Carlos, SP, Brazil.
Ângelo C
ÂC
Perinoto
Rafael M
RM
Maki
Marcelle C
MC
Colhone
Fabiana R
FR
Santos
Vanessa
V
Migliaccio
Katia R
KR
Daghastanli
Rodrigo G
RG
Stabeli
Pietro
P
Ciancaglini
Fernando V
FV
Paulovich
Maria C F
MC
de Oliveira
Osvaldo N
ON
Oliveira
Valtencir
V
Zucolotto
D000818:Animals; D000906:Antibodies / Q000097:blood; D000941:Antigens / Q000737:chemistry / Q000276:immunology; D015374:Biosensing Techniques / Q000379:methods*; D014355:Chagas Disease / Q000175:diagnosis; D050091:Dendrimers / Q000737:chemistry; D055664:Electrochemical Techniques / Q000379:methods; D004566:Electrodes; D055767:Immobilized Proteins / Q000737:chemistry / Q000276:immunology; D007891:Leishmania / Q000276:immunology; D007896:Leishmaniasis / Q000175:diagnosis*; D051379:Mice; D049329:Nanostructures / Q000737:chemistry; D058069:Neglected Diseases / Q000175:diagnosis; D025341:Principal Component Analysis; D014349:Trypanosoma cruzi / Q000276:immunology
D000906:Antibodies; D000941:Antigens; D050091:Dendrimers; D055767:Immobilized Proteins; C104700:PAMAM Starburst