Experimental autoimmune encephalomyelitis (EAE) IN C57Bl/6 mice is not associated with astrogliosis.
The C57Bl/6 mouse is the preferred host for the maintenance of gene deletion mutants and holds a unique place in investigations of cytokine/chemokine networks in neuroinflammation. It is also susceptible to experimental autoimmune encephalomyelitis (EAE), a multiple sclerosis (MS)-like disease commonly used to assess potential MS therapies. Investigations of glial reactivity in EAE have revealed hitherto undescribed astroglial responses in this model, characterized by progressively diminishing glial fibrillary acidic protein and aquaporin-4 immunostaining, from early disease. These observations show that astrocyte responses vary with the EAE paradigm and are an important pathological criterion for disease mapping and therapy evaluation.
Department of Biochemistry, La Trobe University, Victoria 3086, Australia.
Hong
H
Pham
Juliane
J
Doerrbecker
Anton A
AA
Ramp
Claretta S
CS
D'Souza
Dhana G
DG
Gorasia
Anthony W
AW
Purcell
Margaret M
MM
Ayers
Jacqueline M
JM
Orian
D000818:Animals; D051401:Aquaporin 4 / Q000096:biosynthesis; D001253:Astrocytes / Q000378:metabolism / Q000473:pathology; D015153:Blotting, Western; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000378:metabolism / Q000473:pathology*; D005455:Fluorescent Antibody Technique; D005904:Glial Fibrillary Acidic Protein / Q000096:biosynthesis; D005911:Gliosis / Q000378:metabolism / Q000473:pathology*; D007150:Immunohistochemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D016688:Mice, Inbred NOD; D013116:Spinal Cord / Q000378:metabolism / Q000473:pathology
D051401:Aquaporin 4; D005904:Glial Fibrillary Acidic Protein