Testing membrane interactions of CPPs.
The chapter deals with some biophysical methods used for investigating CPP-induced changes in membrane properties by spectroscopy methods such as fluorescence or NMR and methods used for probing CPP-induced leakage in membranes. Some useful model systems for biomembranes are described. These include large unilamellar phospholipid vesicles (LUVs) of well-defined size (diameter typically 100 nm). A protocol for the preparation of such vesicles is included. The leakage studies make use of LUVs with entrapped dye molecules. The NMR studies make use of mixed micelles (bicelles) as a membrane mimetic system, which can be oriented in the magnetic field of the spectrometer.
Department of Biochemistry and Biophysics, Stockholm University, Stockholm, Sweden. astrid@dbb.su.se
Astrid
A
Gräslund
Lena
L
Mäler
D001703:Biophysics / Q000379:methods*; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism*; D057846:Cell-Penetrating Peptides / Q000737:chemistry / Q000378:metabolism*; D005452:Fluoresceins / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008560:Membrane Fluidity; D008958:Models, Molecular; D008968:Molecular Conformation; D011485:Protein Binding; D013050:Spectrometry, Fluorescence; D053835:Unilamellar Liposomes / Q000737:chemistry / Q000378:metabolism
D057846:Cell-Penetrating Peptides; D005452:Fluoresceins; D053835:Unilamellar Liposomes; C007740:fluorexon