Crystallization and preliminary X-ray diffraction analysis of human cytosolic seryl-tRNA synthetase.
Human cytosolic seryl-tRNA synthetase (hsSerRS) is responsible for the covalent attachment of serine to its cognate tRNA(Ser). Significant differences between the amino-acid sequences of eukaryotic, prokaryotic and archaebacterial SerRSs indicate that the domain composition of hsSerRS differs from that of its eubacterial and archaebacterial analogues. As a consequence of an N-terminal insertion and a C-terminal extra-sequence, the binding mode of tRNA(Ser) to hsSerRS is expected to differ from that in prokaryotes. Recombinant hsSerRS protein was purified to homogeneity and crystallized. Diffraction data were collected to 3.13 Å resolution. The structure of hsSerRS has been solved by the molecular-replacement method.
EPSAM and ISTM, Keele University, Staffordshire ST5 5BG, England.
Jean Baptiste
JB
Artero
Susana C M
SC
Teixeira
Edward P
EP
Mitchell
Michael A
MA
Kron
V Trevor
VT
Forsyth
Michael
M
Haertlein
D003460:Crystallization; D018360:Crystallography, X-Ray; D003600:Cytosol / Q000201:enzymology*; D006801:Humans; D012714:Serine-tRNA Ligase / Q000737:chemistry*
D012714:Serine-tRNA Ligase