[Quality specification of Perillae Fructus].
To improve the quality control specification of Perillae Fructus, the identification methods and assay were developed. Rosmarinic acid, luteolin and apigenin in the sample were identified by TLC. The content of rosmarinic acid was determined by HPLC. The linear calibration curve of rosmarinic acid was obtained in the ranges of 19.4-194.2 g x L(-1) (R2 = 0.9999). The arerage coveriy (n=9) for the assay was 99.8% (RSD 3.6%). The established methods are accuracy, sensitivity and reproducible, and can be used for the quality control of Perillae Fructus.
MOE Key Laboratory for Standardization of Chinese Medicines and State Administration of Traditional Chinese Medicine Key Laboratory for New Resources and Quality Evaluation of Chinese Materia Medica, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201210, China. gulihua2008@hotmail.com
Lihua
L
Gu
Chen
C
Lin
Tao
T
Wu
Guixin
G
Chou
Zhengtao
Z
Wang
D047310:Apigenin / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D002934:Cinnamates / Q000032:analysis; D053630:Depsides / Q000032:analysis; D047311:Luteolin / Q000032:analysis; D031344:Perilla frutescens / Q000737:chemistry*; D015203:Reproducibility of Results; D000097123:Rosmarinic Acid
D047310:Apigenin; D002934:Cinnamates; D053630:Depsides; D047311:Luteolin; D000097123:Rosmarinic Acid