Au@Pt nanostructures as oxidase and peroxidase mimetics for use in immunoassays.
In this paper, we demonstrated that Au nanorods coated with a shell composed of Pt nanodots (Au@Pt nanostructures) exhibited intrinsic oxidase-like, peroxidase-like and catalase-like activity, catalyzing oxygen and hydrogen peroxide reduction and the dismutation decomposition of hydrogen peroxide to produce oxygen. Based on these findings, we established an Au@Pt nanostructures based enzyme linked immunosorbent assay (ELISA) for the detection of mouse interleukin 2 (IL-2). In comparison with natural enzymes, Au@Pt nanostructures have advantages of low cost, easy preparation, better stability, and tunable catalytic activity (compared with HRP), which make them a promising enzyme mimetic candidate and may find potential applications in biocatalysis, bioassays, and nano-biomedicine such as reactive oxygen species (ROS)-related fields (anti-aging and therapeutics for neurodegenerative diseases and cancers).
CAS Key Laboratory of Standardization and Measurement for Nanotechnology, National Center for Nanoscience and Technology, Beijing 100190, PR China.
Weiwei
W
He
Ying
Y
Liu
Jinshan
J
Yuan
Jun-Jie
JJ
Yin
Xiaochun
X
Wu
Xiaona
X
Hu
Ke
K
Zhang
Jianbo
J
Liu
Chunying
C
Chen
Yinglu
Y
Ji
Yuting
Y
Guo
D000818:Animals; D002374:Catalase / Q000378:metabolism; D006046:Gold / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry; D007118:Immunoassay / Q000379:methods*; D008422:Materials Testing; D051379:Mice; D015394:Molecular Structure; D049329:Nanostructures / Q000737:chemistry*; D043942:Nanotubes / Q000737:chemistry; D016877:Oxidants / Q000737:chemistry; D010100:Oxygen / Q000378:metabolism; D010984:Platinum / Q000737:chemistry*
D016877:Oxidants; D010984:Platinum; D006046:Gold; D006861:Hydrogen Peroxide; D002374:Catalase; D010100:Oxygen