Use of a Gluconobacter frateurii mutant to prevent dihydroxyacetone accumulation during glyceric acid production from glycerol.
To prevent dihydroxyacetone (DHA) by-production during glyceric acid (GA) production from glycerol using Gluconobacter frateurii, we used a G. frateurii THD32 mutant, ΔsldA, in which the glycerol dehydrogenase subunit-encoding gene (sldA) was disrupted, but ΔsldA grew much more slowly than the wild type, growth starting after a lag of 3 d under the same culture conditions. The addition of 1% w/v D-sorbitol to the medium improved both the growth and the GA productivity of the mutant, and ΔsldA produced 89.1 g/l GA during 4 d of incubation without DHA accumulation.
Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, Japan. hiroshi.habe@aist.go.jp
Hiroshi
H
Habe
Yuko
Y
Shimada
Tokuma
T
Fukuoka
Dai
D
Kitamoto
Masayuki
M
Itagaki
Kunihiro
K
Watanabe
Hiroshi
H
Yanagishita
Toshiharu
T
Yakushi
Kazunobu
K
Matsushita
Keiji
K
Sakaki
D004098:Dihydroxyacetone / Q000378:metabolism*; D020576:Gluconobacter / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D005988:Glyceric Acids / Q000378:metabolism*; D005990:Glycerol / Q000378:metabolism*; D050505:Mutant Proteins / Q000378:metabolism*; D009154:Mutation; D013401:Sugar Alcohol Dehydrogenases / Q000235:genetics
D005988:Glyceric Acids; D050505:Mutant Proteins; C042971:glyceric acid; D013401:Sugar Alcohol Dehydrogenases; C020672:glycerol dehydrogenase; D004098:Dihydroxyacetone; D005990:Glycerol