Novel insights into the use of Glowing LNA as nucleic acid detection probes--influence of labeling density and nucleobases.
Appropriately designed 2'-N-(pyren-1-yl)carbonyl-2'-amino-LNA (locked nucleic acid) display large increases in fluorescence intensity and remarkably high quantum yields upon hybridization with nucleic acid targets. Thermal denaturation and fluorescence spectroscopy studies on ONs modified with known thymine monomer X and novel 5-methylcytosine monomer Y provide new insights into the design principles and mechanism of these Glowing LNA nucleic acid detection probes.
Department of Chemistry, University of Idaho, Moscow, Idaho 83844-2343, USA.
Michael E
ME
Østergaard
Jyotirmoy
J
Maity
B Ravindra
BR
Babu
Jesper
J
Wengel
Patrick J
PJ
Hrdlicka
D044503:5-Methylcytosine / Q000737:chemistry; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization; D015341:Nucleic Acid Probes / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry*; D011721:Pyrenes / Q000737:chemistry; D011789:Quantum Theory; D013050:Spectrometry, Fluorescence; D013941:Thymine / Q000737:chemistry; D044366:Transition Temperature
D015341:Nucleic Acid Probes; D009841:Oligonucleotides; D011721:Pyrenes; C527207:amino-LNA; D044503:5-Methylcytosine; C030984:pyrene; D013941:Thymine