2-Aminoethoxydiphenyl borate (2-APB) reduces respiratory burst, MMP-9 release and CD11b expression, and increases l-selectin shedding in bovine neutrophils.
This study describes the effect of 2-aminoethoxydiphenyl borate (2-APB), a putative store-operated calcium (Ca(2+)) entry (SOCE) inhibitor, on reactive oxygen species (ROS) production, matrix metalloproteinase 9 (MMP-9) release, CD11b and l-selectin (CD62L) expression, size changes and apoptosis in bovine neutrophils stimulated with platelet-activating factor (PAF). It was observed that doses ⩾1μM 2-APB significantly reduced ROS production, whereas 50 and 100μM 2-APB reduced MMP-9 release induced by PAF. Moreover, concentrations ⩾10μM 2-APB reduced CD11b expression and increased l-selectin shedding. PAF induced size changes in neutrophils, and this effect was inhibited by 2-APB. From this work it is possible to conclude that 2-APB at concentrations that inhibit SOCE responses was able to inhibit ROS and MMP-9 release and CD11b expression, and increase l-selectin shedding, suggesting that the Ca(2+) channel involved in SOCE is a potential target for the development of new anti-inflammatory drugs in cattle.
Universidad Austral de Chile, Valdivia, Chile.
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Jara
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D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001896:Boron Compounds / Q000008:administration & dosage / Q000494:pharmacology*; D039481:CD11b Antigen / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D002121:Calcium Channel Blockers / Q000008:administration & dosage / Q000494:pharmacology*; D020013:Calcium Signaling / Q000187:drug effects; D002417:Cattle / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D005434:Flow Cytometry / Q000662:veterinary; D019041:L-Selectin / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D010972:Platelet Activating Factor / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D016897:Respiratory Burst / Q000187:drug effects
D001896:Boron Compounds; D039481:CD11b Antigen; D002121:Calcium Channel Blockers; D010972:Platelet Activating Factor; D017382:Reactive Oxygen Species; D019041:L-Selectin; C109986:2-aminoethoxydiphenyl borate; D020780:Matrix Metalloproteinase 9; D002118:Calcium