Asian dust and titanium dioxide particles-induced inflammation and oxidative DNA damage in C57BL/6 mice.
Inhaled particulate matter (PM) might influence many adverse health effects in human body, including increased exacerbations of pulmonary and cardiovascular diseases. In this study, we examined the associations between PM and pulmonary adverse effects. Two types of particles, Asian dust (AD) and titanium dioxide (TiO(2)), were administered intratracheally to C57BL/6 mice. The mice were exposed to saline and saline suspensions of 20 mg/kg of AD, TiO(2) particles twice a week for 12 weeks. Following exposure with these particles, the lungs were analyzed histopathologically by hematoxylin and eosin (H&E) and Masson's trichrome (MT) staining. Oxidative injuries were determined by immunohistochemistry (IHC) for 8-oxoguanine in the lungs and Comet assays in peripheral blood mononuclear cells (PBMCs) of C57BL/6 mice. Mice exposed to AD and TiO(2) showed significant inflammatory changes and oxidative damages in the lungs as compared with the control group. DNA damage in PBMCs was also increased significantly in AD and TiO(2)-exposed mice. However, lung fibrosis was minimal and there was no significant difference between PM exposed and control mice. Exposure to AD and TiO(2) particles-induced similar inflammatory damages in the lungs and elicited oxidative DNA damage in the PBMCs.
Division of Biological Science, Gachon University of Medicine and Science, 534-2 Yeonsu-dong, Yeonsu-gu, Incheon, Republic of Korea.
You Jin
YJ
Hwang
Ye Sul
YS
Jeung
Min Hae
MH
Seo
Jin Young
JY
Yoon
Dae Young
DY
Kim
Jeong-Woong
JW
Park
Joung Ho
JH
Han
Sung Hwan
SH
Jeong
D000393:Air Pollutants / Q000633:toxicity; D000818:Animals; D020552:Comet Assay / Q000379:methods; D004249:DNA Damage; D004391:Dust / Q000032:analysis; D005355:Fibrosis / Q000473:pathology; D006147:Guanine / Q000031:analogs & derivatives / Q000032:analysis; D007249:Inflammation / Q000139:chemically induced*; D007963:Leukocytes, Mononuclear / Q000737:chemistry*; D008168:Lung / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018384:Oxidative Stress; D010316:Particle Size; D052638:Particulate Matter / Q000633:toxicity*; D014025:Titanium / Q000633:toxicity*
D000393:Air Pollutants; D004391:Dust; D052638:Particulate Matter; C009495:titanium dioxide; C024829:8-hydroxyguanine; D006147:Guanine; D014025:Titanium