Effects of treadmill exercise combined with MK 801 treatment on neuroblast differentiation in the dentate gyrus in rats.
N-methyl-D-aspartate receptor (NR) is involved in activity-dependent synaptic plasticity, such as associative long-term potentiation, and in related central functions, such as learning and memory. In this study, we observed effects of treadmill exercise on NR1 and doublecortin (DCX, a marker for neuroblast differentiation) in the subgranular zone of the dentate gyrus (DG). At 6 weeks of age, rats were put on a treadmill with or without running for 1 h/day for 5 consecutive days at 22 m/min for 5 weeks. Exercise increased NR1 immunoreactivity and protein level in the hippocampus. To identify the correlations between NR and neuroblasts, we intraperitoneally administered a NR antagonist, MK-801, to the exercised rats. MK-801 treatment reduced NR1 protein level in the hippocampus of the exercised rats. In addition, in the MK-801-treated group, the number of DCX cells was significantly decreased in the subgranular zone of the DG. These results suggest that NR may be one of the important factors that modulate neuroblast differentiation during exercise in rats.
Department of Anatomy, College of Veterinary Medicine, Kangwon National University, Chuncheon, South Korea.
Jung Hoon
JH
Choi
Ki-Yeon
KY
Yoo
Choong Hyun
CH
Lee
Sun Shin
SS
Yi
Dae Young
DY
Yoo
Je Kyung
JK
Seong
Yeo Sung
YS
Yoon
In Koo
IK
Hwang
Moo-Ho
MH
Won
D000818:Animals; D015153:Blotting, Western; D002454:Cell Differentiation / Q000187:drug effects*; D018891:Dentate Gyrus / Q000166:cytology* / Q000187:drug effects; D016291:Dizocilpine Maleate / Q000494:pharmacology*; D000089622:Doublecortin Domain Proteins; D000089662:Doublecortin Protein; D005080:Exercise Test; D008297:Male; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D009474:Neurons / Q000166:cytology* / Q000187:drug effects*; D009479:Neuropeptides / Q000378:metabolism; D010805:Physical Conditioning, Animal; D051381:Rats; D017208:Rats, Wistar; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism
C000714848:Dcx protein, rat; D000089622:Doublecortin Domain Proteins; D000089662:Doublecortin Protein; D008869:Microtubule-Associated Proteins; D009479:Neuropeptides; D016194:Receptors, N-Methyl-D-Aspartate; D016291:Dizocilpine Maleate