Effective sampling range of a synthetic protein-based attractant for Ceratitis capitata (Diptera: Tephritidae).
Studies were conducted in Honduras to determine effective sampling range of a female-targeted protein-based synthetic attractant for the Mediterranean fruit fly, Ceratitis capitata (Wiedemann) (Diptera: Tephritidae). Multilure traps were baited with ammonium acetate, putrescine, and trimethylamine lures (three-component attractant) and sampled over eight consecutive weeks. Field design consisted of 38 traps (over 0.5 ha) placed in a combination of standard and high-density grids to facilitate geostatistical analysis, and tests were conducted in coffee (Coffea arabica L.),mango (Mangifera indica L.),and orthanique (Citrus sinensis X Citrus reticulata). Effective sampling range, as determined from the range parameter obtained from experimental variograms that fit a spherical model, was approximately 30 m for flies captured in tests in coffee or mango and approximately 40 m for flies captured in orthanique. For comparison, a release-recapture study was conducted in mango using wild (field-collected) mixed sex C. capitata and an array of 20 baited traps spaced 10-50 m from the release point. Contour analysis was used to document spatial distribution of fly recaptures and to estimate effective sampling range, defined by the area that encompassed 90% of the recaptures. With this approach, effective range of the three-component attractant was estimated to be approximately 28 m, similar to results obtained from variogram analysis. Contour maps indicated that wind direction had a strong influence on sampling range, which was approximately 15 m greater upwind compared with downwind from the release point. Geostatistical analysis of field-captured insects in appropriately designed trapping grids may provide a supplement or alternative to release-recapture studies to estimate sampling ranges for semiochemical-based trapping systems.
USDA-ARS, Subtropical Horticulture Research Station, 13601 Old Cutler Road, Miami, FL 33158, USA. nancy.epsky@ars.usda.gov
Nancy D
ND
Epsky
Hernán R
HR
Espinoza
Paul E
PE
Kendra
Robert
R
Abernathy
David
D
Midgarden
Robert R
RR
Heath
D000818:Animals; D033622:Ceratitis capitata / Q000187:drug effects*; D003069:Coffee / Q000469:parasitology; D004175:Diptera; D005260:Female; D006721:Honduras; D007300:Insect Control / Q000379:methods; D007306:Insecticides / Q000494:pharmacology; D008297:Male; D031022:Mangifera / Q000469:parasitology; D011506:Proteins / Q000138:chemical synthesis / Q000494:pharmacology*
D003069:Coffee; D007306:Insecticides; D011506:Proteins