Biophysical characterization of ovine forestomach extracellular matrix biomaterials.
Ovine forestomach matrix (OFM) is a native and functional decellularized extracellular matrix biomaterial that supports cell adhesion and proliferation and is remodeled during the course of tissue regeneration. Small angle X-ray scattering demonstrated that OFM retains a native collagen architecture (d spacing = 63.5 ± 0.2 nm, orientation index = 20°). The biophysical properties of OFM were further defined using ball-burst, uniaxial and suture retention testing, as well as a quantification of aqueous permeability. OFM biomaterial was relatively strong (yield stress = 10.15 ± 1.81 MPa) and elastic (modulus = 0.044 ± 0.009 GPa). Lamination was used to generate new OFM-based biomaterials with a range of biophysical properties. The resultant multi-ply OFM biomaterials had suitable biophysical characteristics for clinical applications where the grafted biomaterial is under load.
Mesynthes Limited, Lower Hutt 5040, New Zealand.
Evan W
EW
Floden
Sharif F F
SF
Malak
Melissa M
MM
Basil-Jones
Leonardo
L
Negron
James N
JN
Fisher
Stan
S
Lun
Sandi G
SG
Dempsey
Richard G
RG
Haverkamp
Brian R
BR
Ward
Barnaby C H
BC
May
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002448:Cell Adhesion; D049109:Cell Proliferation; D005109:Extracellular Matrix / Q000737:chemistry*; D008422:Materials Testing; D012756:Sheep; D013270:Stomach; D023822:Tissue Engineering
D001672:Biocompatible Materials