Biochemical characterization of UDP-Gal:GlcNAc-pyrophosphate-lipid β-1,4-Galactosyltransferase WfeD, a new enzyme from Shigella boydii type 14 that catalyzes the second step in O-antigen repeating-unit synthesis.
The O antigen is the outer part of the lipopolysaccharide (LPS) in the outer membrane of Gram-negative bacteria and contains many repeats of an oligosaccharide unit. It contributes to antigenic variability and is essential to the full function and virulence of bacteria. Shigella is a Gram-negative human pathogen that causes diarrhea in humans. The O antigen of Shigella boydii type 14 consists of repeating oligosaccharide units with the structure [→6-d-Galpα1→4-d-GlcpAβ1→6-d-Galpβ1→4-d-Galpβ1→4-d-GlcpNAcβ1→]n. The wfeD gene in the O-antigen gene cluster of Shigella boydii type 14 was proposed to encode a galactosyltransferase (GalT) involved in O-antigen synthesis. We confirmed here that the wfeD gene product is a β4-GalT that synthesizes the Galβ1-4GlcNAcα-R linkage. WfeD was expressed in Escherichia coli, and the activity was characterized by using UDP-[³H]Gal as the donor substrate as well as the synthetic acceptor substrate GlcNAcα-pyrophosphate-(CH₂)₁₁-O-phenyl. The enzyme product was analyzed by liquid chromatography-mass spectrometry (LC-MS), high-performance liquid chromatography (HPLC), nuclear magnetic resonance (NMR), and galactosidase digestion. The enzyme was shown to be specific for the UDP-Gal donor substrate and required pyrophosphate in the acceptor substrate. Divalent metal ions such as Mn²(+), Ni²(+), and, surprisingly, also Pb²(+) enhanced the enzyme activity. Mutational analysis showed that the Glu101 residue within a DxD motif is essential for activity, possibly by forming the catalytic nucleophile. The Lys211 residue was also shown to be required for activity and may be involved in the binding of the negatively charged acceptor substrate. Our study revealed that the β4-GalT WfeD is a novel enzyme that has virtually no sequence similarity to mammalian β4-GalT, although it catalyzes a similar reaction.
Department of Medicine, Queen's University, Kingston, Ontario, Canada.
Changchang
C
Xu
Bin
B
Liu
Bo
B
Hu
Yanfang
Y
Han
Lu
L
Feng
John S
JS
Allingham
Walter A
WA
Szarek
Lei
L
Wang
Inka
I
Brockhausen
D002413:Cations, Divalent / Q000378:metabolism; D002853:Chromatography, Liquid; D004252:DNA Mutational Analysis; D020536:Enzyme Activators / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005696:Galactosidases / Q000378:metabolism; D005700:Galactosyltransferases / Q000378:metabolism*; D015870:Gene Expression; D009682:Magnetic Resonance Spectroscopy; D008670:Metals / Q000378:metabolism; D019081:O Antigens / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012761:Shigella boydii / Q000201:enzymology*; D013379:Substrate Specificity; D014531:Uridine Diphosphate Galactose / Q000378:metabolism
D002413:Cations, Divalent; D020536:Enzyme Activators; D008670:Metals; D019081:O Antigens; D011994:Recombinant Proteins; D014531:Uridine Diphosphate Galactose; D005700:Galactosyltransferases; D005696:Galactosidases