[Endophytic fungi from Ginkgo biloba and their biological activities].
OBJECTIVE
To research the isolation method, identification and screen for bioactivities endophytic fungi from ginkgo.


METHODS
Endophytic fungi from ginkgo were separated. By means of microdilution method, activities of endophytes against pathogenic fungi were tested. Then, using DPPH, the antioxidant activities were measured.


RESULTS
Nine strains (16.1%) showed antifungal activities against Candida albicans, Cryptococcus neoformans, Trichophyton rubrum and Aspergillus fumigatus. Among these bioactive strains, the growth of T. rubrum was strongly inhibited by T-1-2-1, as the MIC80 was equal to fluconazole, the positive control. Five strains (8.9%) showed antioxidant activities. Among them sample T-3-2-2 and T-6-5-7 showed the strongest antioxidant activities.


CONCLUSIONS
Endophytic fungi of ginkgo would be potential and rich resources for drug development.
Department of Pharmacognosy, School of Phannrmacy, Second Military Medical University, Shanghai 200433, China.
Hongsheng
H
Yu
Lei
L
Zhang
Lin
L
Li
Wenchao
W
Li
Ting
T
Han
Liangdong
L
Guo
Luping
L
Qin
D000935:Antifungal Agents / Q000494:pharmacology; D001232:Aspergillus fumigatus / Q000187:drug effects; D002176:Candida albicans / Q000187:drug effects; D003455:Cryptococcus neoformans / Q000187:drug effects; D015725:Fluconazole / Q000494:pharmacology; D005658:Fungi / Q000737:chemistry* / Q000187:drug effects / Q000302:isolation & purification*; D020441:Ginkgo biloba / Q000382:microbiology*; D008826:Microbial Sensitivity Tests; D014249:Trichophyton / Q000187:drug effects
D000935:Antifungal Agents; D015725:Fluconazole