Phenolic compounds from Pueraria lobata protect PC12 cells against Aβ-induced toxicity.
Bioassay-guided fractionation of the EtOAc-soluble extract of Pueraria lobata based on the inhibition of Aβ-induced toxicity in PC12 cells resulted in the isolation of four known active compounds, genistein (8), biochanin A (9), sissotrin (10), and puerol B (11). Of these, genistein (8) and biochanin A (9) exhibited potent neuroprotective effects with ED(50) values of 33.7 and 27.8 μM, respectively. In addition, a new coumestan, 2-(α,α-dimethylallyl)coumestrol (1) was isolated and characterized, but proved to be inactive, as were additional seven known compounds. The structure of new compound 1 was determined using spectroscopic techniques.
School of Life Sciences and Biotechnology, Korea University, Seoul, Korea.
Yun-hyeok
YH
Choi
Seong Su
SS
Hong
Yu Su
YS
Shin
Bang Yeon
BY
Hwang
So-Young
SY
Park
Dongho
D
Lee
D000544:Alzheimer Disease / Q000188:drug therapy / Q000517:prevention & control; D016229:Amyloid beta-Peptides / Q000378:metabolism*; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D003375:Coumestrol / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D055808:Drug Discovery; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D019833:Genistein / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D020128:Inhibitory Concentration 50; D009682:Magnetic Resonance Spectroscopy; D008518:Medicine, East Asian Traditional; D015394:Molecular Structure; D009474:Neurons / Q000187:drug effects*; D018696:Neuroprotective Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D016716:PC12 Cells; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry; D059808:Polyphenols; D029908:Pueraria / Q000737:chemistry*; D051381:Rats; D021241:Spectrometry, Mass, Electrospray Ionization
D016229:Amyloid beta-Peptides; D005419:Flavonoids; D018696:Neuroprotective Agents; D010636:Phenols; D010936:Plant Extracts; D059808:Polyphenols; D019833:Genistein; C004541:biochanin A; D003375:Coumestrol