DNA-based diagnostic tests for Salmonella strains targeting hilA, agfA, spvC and sef genes.
The goal of this study was to evaluate the suitability of the hilA, agfA, spvC and sef genes amplification by PCR as a method for detection of Salmonella strains. Twenty nine isolates of Salmonella spp. including 6 different serotypes were analyzed in this study. The bacteria were isolated between 2005 and 2007 and serotyped at the Clinical Hospital of Infectious Disease, Cluj-Napoca. Ten non-Salmonella strains were also tested by the same procedure. We used a direct PCR technique, DNA extraction had been skipped and the bacterial cell wall denaturated in the first step of the reaction. All Salmonella strains gave positive results by the PCR amplification of hilA gene. The utilization of the sef, and spvC genes or spvC and agfA genes in a multiplex PCR provides a valuable diagnostic tool for Salmonella enteritidis strains.
Babeş-Bolyai University, Faculty of Biology and Geology, 400006-Cluj-Napoca, Romania. dancor78@yahoo.com
Cornelia
C
Crăciunaş
Anca-Livia
AL
Keul
Mirela
M
Flonta
Mariana
M
Cristea
D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D019757:Carbon-Oxygen Lyases / Q000235:genetics*; D036781:Fimbriae Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods*; D012475:Salmonella / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D012477:Salmonella enteritidis / Q000235:genetics / Q000302:isolation & purification; D012703:Serotyping; D015534:Trans-Activators / Q000235:genetics*
C107306:AgfA protein, Salmonella enterica; D001426:Bacterial Proteins; C102236:HilA protein, Salmonella; D015534:Trans-Activators; C080933:sefC protein, Salmonella enteritidis; D036781:Fimbriae Proteins; D019757:Carbon-Oxygen Lyases; C527194:phosphothreonine lyase, Salmonella enterica