Differential protein expression level identification by knockout of 14-3-3τ with siRNA technique and 2DE followed MALDI-TOF-TOF-MS.
Cytotrophoblast cell differentiation into syncytiotrophoblast cells is a complex and delicate process, and the mechanism needs a large number of further studies. Knockout of 14-3-3τ expression and some further investigations were performed in the syncytiotrophoblast cell formation with RNA interference (RNAi) technology. The study found that the 14-3-3τ expression level with the formation of syncytiotrophoblast cells increased. Hypoxia inhibited the formation of syncytiotrophoblast cells where 14-3-3τ expression level decreased. RNAi reduced 14-3-3τ expression, and reduced the formation of syncytiotrophoblast cells. So that 14-3-3τ is not only involved in the cell regulation, but also in the formation of syncytiotrophoblast cells. 14-3-3τ is an important regulatory factor which inhibits the formation of hypoxia.
Department of Chemistry, Fudan University, 220 Handan Road, Shanghai 200433, China.
Hong
H
Jin
Rong
R
Hu
Yan
Y
Cheng
Fengying
F
Yang
Xinwen
X
Zhou
Xiaotian
X
Li
Peng-yuan
PY
Yang
D048948:14-3-3 Proteins / Q000235:genetics*; D015153:Blotting, Western; D002454:Cell Differentiation; D015687:Cell Hypoxia; D002460:Cell Line; D015180:Electrophoresis, Gel, Two-Dimensional; D005786:Gene Expression Regulation; D055786:Gene Knockout Techniques; D006801:Humans; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014327:Trophoblasts / Q000166:cytology* / Q000378:metabolism*
D048948:14-3-3 Proteins; D034741:RNA, Small Interfering