Hemoglobin expression in rat experimental granulation tissue.
The general opinion that hemoglobin is only a carrier protein for oxygen and carbon dioxide has been challenged by several recent studies showing hemoglobin expression in other cells than those of the erythroid series, for example, in macrophages. We discovered β-globin expression in rat experimental granulation tissue induced by subcutaneously implanted cellulose sponges. Closer investigation revealed also α-globin expression. The first peak of the biphasic globin expression noticed during granulation tissue formation correlated with the invasion of monocytes/macrophages, whereas the second one seemed to be connected to the appearance of hematopoietic progenitors. Data presented in this study indicate globin expression both in macrophages and in immature erythroid cells as validated by erythroid-specific markers.
Department of Medical Biochemistry and Genetics, University of Turku, Turku, Finland.
Miretta
M
Tommila
Christoffer
C
Stark
Anne
A
Jokilammi
Ville
V
Peltonen
Risto
R
Penttinen
Erika
E
Ekholm
D000818:Animals; D041905:Erythroid Cells / Q000378:metabolism; D004920:Erythropoiesis / Q000235:genetics; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006097:Granulation Tissue / Q000378:metabolism*; D006454:Hemoglobins / Q000235:genetics / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism; D008297:Male; D019001:Nitric Oxide Synthase / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D055542:alpha-Globins / Q000235:genetics / Q000378:metabolism; D055544:beta-Globins / Q000235:genetics / Q000378:metabolism
D006454:Hemoglobins; D012333:RNA, Messenger; D055542:alpha-Globins; D055544:beta-Globins; D019001:Nitric Oxide Synthase