Tailoring silver nanodots for intracellular staining.
Through tailored oligonucleotide scaffolds, Ag nanocluster syntheses have yielded thermally and cell-culture medium stable silver cluster-based emitters. Optimizing ssDNA stability has enabled creation of highly concentrated and spectrally pure nanocluster emitters with strong intracellular emission. Both fixed and live-cell staining become possible, and intracellular delivery is demonstrated both through conjugation to cell-penetrating peptides and via microinjection.
School of Chemistry and Biochemistry and Parker H. Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, 901 Atlantic Drive, Atlanta, GA 30332-0400, USA.
Sungmoon
S
Choi
Junhua
J
Yu
Sandeep A
SA
Patel
Yih-Ling
YL
Tzeng
Robert M
RM
Dickson
D000818:Animals; D057846:Cell-Penetrating Peptides / Q000737:chemistry*; D004277:DNA, Single-Stranded / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000528:radiation effects; D051379:Mice; D018613:Microscopy, Confocal; D041681:NIH 3T3 Cells; D012834:Silver / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D013194:Staining and Labeling
D057846:Cell-Penetrating Peptides; D004277:DNA, Single-Stranded; D012834:Silver