Adenovirus vector production and purification.
Replication deficient adenovirus vectors are frequently used tools for the delivery of transgenes in vitro and in vivo. In addition, several therapeutic products based on adenovirus are under clinical development. This review outlines adenovirus vector production discussing different vector types, available production cell lines and state of the art of production process development and purification.
IBET/ITQB-UNL, Apartado 12, P-2781-901, Oeiras, Portugal.
Ana Carina
AC
Silva
Cristina
C
Peixoto
Tanja
T
Lucas
Claudia
C
Küppers
Pedro E
PE
Cruz
Paula M
PM
Alves
Stefan
S
Kochanek
D000256:Adenoviridae / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification*; D000818:Animals; D002460:Cell Line; D004261:DNA Replication; D005822:Genetic Vectors / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D019076:Transgenes; D014779:Virus Replication