Immunohistochemical evidence of synaptic retraction, cytoarchitectural remodeling, and cell death in the inner retina of the rat model of oygen-induced retinopathy (OIR).
OBJECTIVE
Postnatal exposure to hyperoxia destroys the plexiform layers of the neonatal rat retina, resulting in significant electroretinographic anomalies. The purpose of this study was to identify the mechanisms at the origin of this loss.


METHODS
Sprague-Dawley (SD) and Long Evans (LE) rats were exposed to hyperoxia from birth to postnatal day (P) 6 or P14 and from P6 to P14, after which rats were euthanatized at P6, P14, or P60.


RESULTS
At P60, synaptophysin staining confirmed the lack of functional synaptic terminals in SD (outer plexiform layer [OPL]) and LE (OPL and inner plexiform layer [IPL]) rats. Uneven staining of ON-bipolar cell terminals with mGluR6 suggests that their loss could play a role in OPL thinning. Protein kinase C(PKC)-α and recoverin (rod and cone ON-bipolar cells, respectively) showed a lack of dendritic terminals in the OPL with disorganized axonal projections in the IPL. Although photoreceptor nuclei appeared intact, a decrease in bassoon staining (synaptic ribbon terminals) suggests limited communication to the inner retina. Findings were significantly more pronounced in LE rats. An increase in TUNEL-positive cells was observed in LE (inner nuclear layer [INL] and outer nuclear layer [ONL]) and SD (INL) rats after P0 to P14 exposure (425.3%, 102.2%, and 146.3% greater than control, respectively [P < 0.05]).


CONCLUSIONS
Results suggest that cell death and synaptic retraction are at the root of OPL thinning. Increased TUNEL-positive cells in the INL confirm that cells die, at least in part, because of apoptosis. These findings propose a previously undescribed mechanism of cell death and synaptic retraction that are likely at the origin of the functional consequences of hyperoxia.
Department of Pharmacology, McGill University-Montreal Children’s Hospital Research Institute, Montreal, Quebec, Canada.
Allison Lindsay
AL
Dorfman
Nicolás
N
Cuenca
Isabel
I
Pinilla
Sylvain
S
Chemtob
Pierre
P
Lachapelle
D000818:Animals; D000831:Animals, Newborn; D017209:Apoptosis; D015415:Biomarkers / Q000378:metabolism; D004195:Disease Models, Animal; D005136:Eye Proteins / Q000378:metabolism; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D018496:Hyperoxia / Q000378:metabolism* / Q000473:pathology; D020287:In Situ Nick-End Labeling; D007231:Infant, Newborn; D008297:Male; D018613:Microscopy, Confocal; D009473:Neuronal Plasticity / Q000502:physiology*; D010100:Oxygen / Q000633:toxicity; D017729:Presynaptic Terminals / Q000378:metabolism*; D051381:Rats; D020318:Rats, Long-Evans; D017207:Rats, Sprague-Dawley; D055351:Retinal Neurons / Q000502:physiology*; D012178:Retinopathy of Prematurity / Q000378:metabolism* / Q000473:pathology
D015415:Biomarkers; D005136:Eye Proteins; D010100:Oxygen