In vitro antitumor and immunomodulatory effects of the protein PCP-3A from mushroom Pleurotus citrinopileatus.
A nonlectin glycoprotein (PCP-3A) newly isolated from the fruit body of edible golden oyster mushroom Pleurotus citrinopileatus has been shown to be growth inhibitory against human myeloid leukemic U937 cells in a previous report. There is a well-recognized relation between antitumor activity and immunomodulation. The immunomodulatory activity of PCP-3A was therefore assessed in the present study. Human mononuclear cells (MNC) and the CD4(+) T lymphocytes isolated from them were stimulated separately with PCP-3A for various durations and then filtered to obtain the conditioned media (CM). The conditioned medium from MNC (MNC-CM) was proved effective in inhibiting the growth of U937 cells. Increased secretion of cytokines TNF-α, IL-2, and IFN-γ from the stimulated MNC and CD4(+) T cells was found in CM. The antibody neutralization test of MNC-CM revealed that the growth inhibition on leukemic U937 cells was related to the elevation in cytokine concentration. We propose that PCP-3A stimulated human MNC to secrete cytokines TNF-α, IL-2, and IFN-γ, which subsequently inhibit the growth of U937 cells, and that PCP-3A may be a possible material for developing into an antileukemia ingredient in health food.
Graduate Institute of Food Science and Technology, National Taiwan University, Taipei 106, Taiwan.
Jian-Nan
JN
Chen
Chia-Yu
CY
Ma
Pei-Feng
PF
Tsai
Yuh-Tai
YT
Wang
James Swi-Bea
JS
Wu
D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects / Q000276:immunology; D049109:Cell Proliferation / Q000187:drug effects; D016207:Cytokines / Q000276:immunology; D006023:Glycoproteins / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D007155:Immunologic Factors / Q000737:chemistry / Q000494:pharmacology*; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000276:immunology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D010940:Plant Proteins / Q000737:chemistry / Q000494:pharmacology*; D020076:Pleurotus / Q000737:chemistry*; D020298:U937 Cells; D014675:Vegetables / Q000737:chemistry*
D000970:Antineoplastic Agents; D016207:Cytokines; D006023:Glycoproteins; D007155:Immunologic Factors; D010936:Plant Extracts; D010940:Plant Proteins