Monoclonal antibodies against excretory/secretory antigens of Angiostrongylus cantonensis.
In the present study, four murine monoclonal antibodies (MAbs) were generated against the excretory/secretory (ES) products of Angiostrongylus cantonensis adult worms; two represented IgG1 and two represented IgM MAbs, and they were designated 12D5, 15F8, 21B7 and 14G10, respectively. Immunoblotting revealed that all of the MAbs predominantly recognized a 98 kDa antigen in the ES products of A. cantonensis adult worms, and no cross reactions were found with the whole worm antigens of some other common parasites, namely, Schistosoma japonicum, Clonorchis sinensis, Paragonimus westermani, Ascaris lumbricoides, Trichinella spiralis, Anisakis sp., Echinococcus granulosus, Taenia solium, and Spirometra erinacei. Immunolocalization showed that all of the four MAbs reacted with the cuticle of the adult parasite, the external surface of its intestinal canal and reproductive organs, and its egg and first-stage larvae in the lungs of rats experimentally infected with A. cantonensis. The generation and characterization of four specific MAbs against A. cantonensis ES antigens provide foundation for the development of specific immunological diagnostic techniques for human infections with A. cantonensis.
Department of Parasitology, College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province, PR China.
Mu-Xin
MX
Chen
Ren-Li
RL
Zhang
Jia-Xu
JX
Chen
Shao-Hong
SH
Chen
Xiao-Heng
XH
Li
Shi-Tong
ST
Gao
Yi-Jie
YJ
Geng
Da-Na
DN
Huang
Lin
L
Ai
Min-Jun
MJ
Xu
Xing-Quan
XQ
Zhu
D017185:Angiostrongylus cantonensis / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology / Q000378:metabolism; D000947:Antigens, Helminth / Q000276:immunology*; D015153:Blotting, Western; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000276:immunology; D007075:Immunoglobulin M / Q000096:biosynthesis / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009349:Nematode Infections / Q000175:diagnosis / Q000276:immunology / Q000378:metabolism; D051381:Rats; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth; D007074:Immunoglobulin G; D007075:Immunoglobulin M