Genetic modification of chondrocytes using viral vectors.
The use of isolated cells to construct engineered tissues provides the opportunity to genetically modify those cells prior to the formation of tissue. This should make it possible to create transgenic human model tissues that can be used to determine gene function as well as to identify or validate potential therapeutic targets. As proof of principle, we have used RNA interference to selectively suppress the expression of aggrecanase genes in human chondrocytes, in an attempt to determine which of these key enzymes have roles in arthritic cartilage destruction. This combination of gene targeting and tissue engineering we are using should be equally applicable to the identification of gene function in other biological systems.
Faculty of Medicine & Health Sciences, University of Nottingham, Nottingham, UK.
Teresa
T
Coughlan
Aileen
A
Crawford
Paul
P
Hatton
Michael
M
Barker
D051722:ADAM Proteins / Q000378:metabolism; D000818:Animals; D019902:Chondrocytes / Q000201:enzymology / Q000378:metabolism*; D003001:Cloning, Molecular; D055785:Gene Knockdown Techniques; D005821:Genetic Techniques; D005822:Genetic Vectors / Q000235:genetics*; D006801:Humans; D010957:Plasmids / Q000235:genetics / Q000302:isolation & purification; D012313:RNA / Q000302:isolation & purification; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D012190:Retroviridae / Q000235:genetics*; D014161:Transduction, Genetic; D014162:Transfection
D034741:RNA, Small Interfering; D012313:RNA; D051722:ADAM Proteins