[Assay of andrographolide bulk drug by HPLC].
OBJECTIVE
To establish a method for the determination of andrographolide bulk drug by HPLC.


METHODS
The condition of HPLC was Diamonsil C18 (2) column (4.6 mm x 250 mm, 5 microm) with methanol-water (60:40) as a mobile phase. The flow rate was 1.0 mL x min(-1) and the detection wavelength was 235 nm.


RESULTS
The standard curve was linear in the range of 0.5-5.0 microg and the correlation coefficient was 1.000. The average recoveries of the low, middle and high concentrations were 99.1%, 99.5% and 100.8%, respectively.


CONCLUSIONS
The method is simple, precise and accurate, and it can be used for the quality control of andrographolide bulk drug.
State Key Laboratory of Natural and Biomimetic Drugs, Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China.
Wei
W
Xu
Xiuwei
X
Yang
D002851:Chromatography, High Pressure Liquid; D004224:Diterpenes / Q000032:analysis*; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D015203:Reproducibility of Results
D004224:Diterpenes; D004365:Drugs, Chinese Herbal; C030419:andrographolide