Prolonged shedding of amantadine- and oseltamivir-resistant influenza A(H3N2) virus with dual mutations in an immunocompromised infant.
In this study, we report a case of multidrug-resistant influenza A(H3N2) virus isolated from an immunosuppressed patient with prolonged viral shedding. We also describe the genetic characterization of the haemagglutinin, neuraminidase and M2 influenza genes. The virus contained the substitutions E119V in neuraminidase and V27A in M2, which produce resistance to oseltamivir and adamantanes, respectively. This is the first report of this dual mutation pattern in multidrug-resistant influenza A(H3N2) virus.
Centro Nacional de Microbiología, Instituto de Salud Carlos III, Madrid, Spain. guille2003@hotmail.com
Guillermo
G
Ruiz-Carrascoso
Inmaculada
I
Casas
Francisco
F
Pozo
Marta
M
González-Vincent
Pilar
P
Pérez-Breña
D000547:Amantadine / Q000627:therapeutic use*; D000595:Amino Acid Sequence; D000998:Antiviral Agents / Q000627:therapeutic use; D024921:Drug Resistance, Multiple, Viral; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics; D006801:Humans; D016867:Immunocompromised Host; D007223:Infant; D053122:Influenza A Virus, H3N2 Subtype / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D007251:Influenza, Human / Q000188:drug therapy / Q000821:virology; D008297:Male; D008969:Molecular Sequence Data; D009154:Mutation; D009439:Neuraminidase / Q000235:genetics; D053139:Oseltamivir / Q000627:therapeutic use*; D014763:Viral Matrix Proteins / Q000235:genetics; D000086082:Viroporin Proteins
D000547:Amantadine; D000998:Antiviral Agents; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D009439:Neuraminidase; D053139:Oseltamivir; D014763:Viral Matrix Proteins; C488937:M2 protein, Influenza A virus; D000086082:Viroporin Proteins