[Localization and expression of CLIC1 in hepatocarcinoma ascites cell lines with high or low potentials of lymphatic spread].
OBJECTIVE
To study the localization and expression of CLIC1 in mouse hepatocarcinoma ascites cell lines with different metastatic potentials.


METHODS
Mouse hepatocarcinoma ascites models (a high potential of lymphatic metastasis cell line-Hca-F, and a low potential of lymphatic metastasis cell line-Hca-P) were investigated using fluorescent two-dimensional difference-gel electrophoresis (2-D DIGE) and mass spectrometry for detecting the localization and expression of CLIC1. Immunofluorescence, immunocytochemistry and Western blot were used to assess CLIC1 protein status in the two cell lines.


RESULTS
CLIC1 expression was obtained in the cytoplasm and plasma membrane of cells in both cell lines. 2-D DIGE showed that CLIC1 was overexpressed in Hca-F cells, 1.6 folds higher than that of the Hca-P cells. Hca-F cells also had a higher integral membrane CLIC1 in the Hca-P cells.


CONCLUSIONS
Although CLIC1 expression is detected in both Hca-F and Hca-P cell lines, a higher protein expression level is present in Hca-F cells. CLIC1 may play an important role in tumor metastasis.
Department of Pathology, Dalian Medical University, Dalian 116044, China.
Mei-ying
MY
Song
Jian-wu
JW
Tang
Ming-zhong
MZ
Sun
Shu-qing
SQ
Liu
Bo
B
Wang
D000818:Animals; D001201:Ascites / Q000378:metabolism* / Q000473:pathology; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism; D018118:Chloride Channels / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism; D007150:Immunohistochemistry; D008114:Liver Neoplasms, Experimental / Q000378:metabolism* / Q000473:pathology; D008207:Lymphatic Metastasis; D051379:Mice; D008815:Mice, Inbred Strains; D057825:Two-Dimensional Difference Gel Electrophoresis
D018118:Chloride Channels; C501967:Clic1 protein, mouse