Metagenomic characterization of oyster shell dump reveals predominance of Firmicutes bacteria.
Metagenomic analyses were conducted to evaluate the biodiversity of oyster shell bacteria, under storage conditions, on the basis of 16s rDNA sequences. Temperature was recorded during a one year storage period, and the highest temperature (about 60 degrees C) was observed after five months ofstorage. Bacterial diversity was greatest in the initial stage sample, with 33 different phylotypes classified under seven phyla (Proteobacteria, Bacteroidetes, Firmicutes, Actinobacteria, Planctomycetes, Verrucomicrobia and unclassified bacteria), with 42.22% ofphylotypes belonging to Proteobacteria. The lowest diversity was found in the high temperature (fermentation) stage sample, with 10 different phylotypes belonging to Firmicutes (78.57%) and Bacteroidetes. In the final stage sample, bacteria were found belonging to Proteobacteria, Bacteroidetes, and Firmicutes, and some were unclassified bacteria. Of the bacteria constituting the final stage metagenome, 69.70% belonged to Firmicutes. Our results show that bacteria belonging to phylum Firmicutes were predominant during fermentation, and during the final stages of oyster shell storage, which suggests that these bacteria supposed to be the key players for oyster shell biodegradation.
Division of Applied Life Sciences (BK21 Program), Gyeongsang National University, Chinju 660-701, Republic of Korea.
Renukaradhya K
RK
Math
Shah Md Asraful
SM
Islam
Sun Joo
SJ
Hong
Kye Man
KM
Cho
Jong Min
JM
Kim
Myoung Geun
MG
Yun
Ji Joong
JJ
Cho
Eun Jin
EJ
Kim
Young Han
YH
Lee
Han Dae
HD
Yun
D000818:Animals; D001673:Biodegradation, Environmental; D006094:Gram-Positive Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D054892:Metagenome; D010125:Ostreidae / Q000033:anatomy & histology / Q000382:microbiology*; D010802:Phylogeny; D056910:Republic of Korea; D014866:Waste Products
D014866:Waste Products