In vitro and in vivo assays of protein kinase CK2 activity.
Protein kinase CK2 (formerly casein kinase 2) is recognized as a central component in the control of the cellular homeostasis; however, much remains unknown regarding its regulation and its implication in cellular transformation and carcinogenesis. Moreover, study of CK2 function and regulation in a cellular context is complicated by the dynamic multisubunit architecture of this protein kinase. Although a number of robust techniques are available to assay CK2 activity in vitro, there is a demand for sensitive and specific assays to evaluate its activity in living cells. We hereby provide a detailed description of several assays for monitoring the CK2 activity and its subunit interaction in living cells. The guidelines presented herein should enable researchers in the field to establish strategies for cellular screenings of CK2 inhibitors.
INSERM, U873, CEA, iRTSV/LTS, Université Joseph Fourier, Grenoble, France.
Renaud
R
Prudent
Céline F
CF
Sautel
Virginie
V
Moucadel
Béatrice
B
Laudet
Odile
O
Filhol
Claude
C
Cochet
D000818:Animals; D047390:Casein Kinase II / Q000032:analysis* / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods; D021122:Protein Subunits / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D013050:Spectrometry, Fluorescence / Q000379:methods; D014162:Transfection / Q000379:methods
D021122:Protein Subunits; D011994:Recombinant Proteins; D047390:Casein Kinase II