[Heterogeneous organization of a tandem repeat family in subtelomeric heterochromatin of rye].
The presence of tandem repeat multicopy families in subtelomeric regions of all chromosomes is a characteristic feature of the rye karyotype, in contrast to the organization of these regions in chromosomes of extensively studied species, such as human, rice, and arabidopsis. To study the molecular structure of these regions, we analyzed BAC clones from a library constructed from the genetic material of rye chromosome 1 short arm (1RS). Screening of the library detected numerous clones that contained copies of multicopy tandem families of DNA sequences pSc200, pSc250, and pSc119.2. An examination of the molecular organization of tandem stretches of the pSc200 family, which is the most common in the rye genome, showed that the subtelomeric 1RS region includes several such stretches, each of which contains characteristic blocks of multimers of various periodicity. Such pattern of heterogeneous organization of tandem repeat stretches differs from the view of the tandem stretches as monotonous sequence of identical monomers, which was generally accepted in recent past.
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Evtushenko
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Vershinin
D022202:Chromosomes, Artificial, Bacterial; D032461:Chromosomes, Plant / Q000235:genetics*; D015723:Gene Library; D006570:Heterochromatin / Q000235:genetics*; D006801:Humans; D012434:Secale / Q000235:genetics*; D020080:Tandem Repeat Sequences; D016615:Telomere / Q000235:genetics
D006570:Heterochromatin