Optimization of extraction of evodiamine and rutaecarpine from fruit of Evodia rutaecarpa using modified supercritical CO(2).
Evodiamine and rutaecarpine have been intensively studied due to their pharmacological actions and clinical applications. In this report, supercritical fluid was used to extract evodiamine and rutaecarpine from the unripe fruit of Evodia rutaecarpa. Response surface methodology using Box-Behnken experimental design was utilized to optimize parameters for supercritical carbon dioxide extraction with methanol as co-solvent. The effect of various values of dynamic extraction time (30-90min), temperature (50-70°C) and pressure (200-400bar) on extraction yields of the two compounds was evaluated. Determinations of the extracts were performed by high-performance liquid chromatography. The experimental data obtained were fitted to second-order polynomial equations and analyzed by analysis of variance. The highest yields predicted were 1.217mg/g for evodiamine and 0.969mg/g for rutaecarpine at the optimal values (time 78min, temperature 62°C, pressure 280bar and co-solvent flow rate 0.4mL/min), based on the selected range of experimental conditions.
Department of Bioengineering and Pharmaceutics, Ningbo Institute of Technology, Zhejiang University, Ningbo 315100, China. liu_ben0@hotmail.com
Ben
B
Liu
Feng
F
Guo
Yiling
Y
Chang
Hailiang
H
Jiang
Qiang
Q
Wang
D000465:Algorithms; D000704:Analysis of Variance; D002245:Carbon Dioxide / Q000737:chemistry*; D005591:Chemical Fractionation / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D032086:Evodia / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry; D026121:Indole Alkaloids / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D000432:Methanol; D010936:Plant Extracts / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D011312:Pressure; D011799:Quinazolines / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D013696:Temperature; D013997:Time Factors; D052999:Quinazolinones
D002245:Carbon Dioxide; D026121:Indole Alkaloids; D000432:Methanol; D010936:Plant Extracts; D011799:Quinazolines; C028632:rutecarpine; D052999:Quinazolinones; C049639:evodiamine