Effects of berberine on expression of LOX-1 and SR-BI in human macrophage-derived foam cells induced by ox-LDL.
This study investigates the effects of beriberine on the expression of lectin-like ox-LDL receptor-1 (LOX-1), scavenger receptor A (SR-A), SR class B type I (SR-BI) and ATP-binding cassette transporter A1 (ABCA1) in human macrophage-derived foam cells induced by ox-LDL. Different concentrations of Berberine were co-cultured with THP-1 derived foam cells. The mRNA and protein expressions of LOX-1, SR-A, SR-BI and ABCA1 were determined by RT-PCR and Western blot analysis, respectively. Ox-LDL significantly increased the expression of LOX-1 and inhibited the expression of SR-BI in a dose- and time-dependent manner. Berberine significantly inhibited the effects of ox-LDL in a dose- and time-dependent manner. Moreover, ox-LDL significantly promoted ABCA1 expression. However, berberine had no effect on SR-A or ABCA1 expression. Berberine can inhibit the expression of LOX-1 and promote the expression of SR-BI in macrophage-derived foam cells. Therefore, berberine could be used to treat atherosclerotic diseases.
Department of Geriatrics, Union Hospital, Huazhong University of Science and Technology, Wuhan, China. smguan16@yahoo.com
Siming
S
Guan
Bin
B
Wang
Wei
W
Li
Jinghuan
J
Guan
Xin
X
Fang
D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism; D050197:Atherosclerosis / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D001599:Berberine / Q000494:pharmacology* / Q000627:therapeutic use; D015153:Blotting, Western; D002460:Cell Line; D039323:Coptis / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005487:Foam Cells / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D008077:Lipoproteins, LDL / Q000378:metabolism / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D008517:Phytotherapy; D012333:RNA, Messenger / Q000378:metabolism; D051131:Receptors, Oxidized LDL / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D027343:Rhizome; D051117:Scavenger Receptors, Class A / Q000235:genetics / Q000378:metabolism; D051122:Scavenger Receptors, Class B / Q000037:antagonists & inhibitors* / Q000235:genetics
D018528:ATP-Binding Cassette Transporters; D004365:Drugs, Chinese Herbal; D008077:Lipoproteins, LDL; D012333:RNA, Messenger; D051131:Receptors, Oxidized LDL; D051117:Scavenger Receptors, Class A; D051122:Scavenger Receptors, Class B; C099252:oxidized low density lipoprotein; D001599:Berberine