Production of fibrolytic enzymes by Aspergillus japonicus C03 using agro-industrial residues with potential application as additives in animal feed.
Solid-state fermentation obtained from different and low-cost carbon sources was evaluated to endocellulases and endoxylanases production by Aspergillus japonicus C03. Regarding the enzymatic production the highest levels were observed at 30 °C, using soy bran added to crushed corncob or wheat bran added to sugarcane bagasse, humidified with salt solutions, and incubated for 3 days (xylanase) or 6 days (cellulase) with 70% relative humidity. Peptone improved the xylanase and cellulase activities in 12 and 29%, respectively. The optimum temperature corresponded to 60 °C and 50-55 °C for xylanase and cellulase, respectively, both having 4.0 as optimum pH. Xylanase was fully stable up to 40 °C, which is close to the rumen temperature. The enzymes were stable in pH 4.0-7.0. Cu++ and Mn++ increased xylanase and cellulase activities by 10 and 64%, respectively. A. japonicus C03 xylanase was greatly stable in goat rumen fluid for 4 h during in vivo and in vitro experiments.
Departamento de Bioquímica e Imunologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, Brazil.
Fernanda Dell Antonio
FD
Facchini
Ana Claudia
AC
Vici
Victor Ricardo Amin
VR
Reis
João Atilio
JA
Jorge
Héctor Francisco
HF
Terenzi
Ricardo Andrade
RA
Reis
Maria de Lourdes Teixeira de Moraes
Mde L
Polizeli
D000821:Animal Feed; D000818:Animals; D001230:Aspergillus / Q000201:enzymology* / Q000378:metabolism; D002244:Carbon / Q000378:metabolism; D002480:Cellulase / Q000096:biosynthesis* / Q000737:chemistry* / Q000378:metabolism; D043364:Endo-1,4-beta Xylanases / Q000096:biosynthesis* / Q000737:chemistry* / Q000378:metabolism; D004795:Enzyme Stability; D005285:Fermentation; D005503:Food Additives; D005656:Fungal Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism; D006041:Goats; D006863:Hydrogen-Ion Concentration; D007218:Industrial Microbiology; D007220:Industrial Waste / Q000032:analysis; D009584:Nitrogen / Q000378:metabolism / Q000600:supply & distribution; D012417:Rumen / Q000201:enzymology / Q000378:metabolism; D012418:Ruminants; D013696:Temperature
D005503:Food Additives; D005656:Fungal Proteins; D007220:Industrial Waste; D002244:Carbon; D002480:Cellulase; D043364:Endo-1,4-beta Xylanases; D009584:Nitrogen