Proteomic analysis of apoptosis induction in human lung cancer cells by recombinant MVL.
Lung cancer is still difficult to treat by current chemotherapeutic procedures. We recently found that MVL, an anti-HIV lectin from blue-green algae Microcystis viridis, also has antitumor activity. The objective of this study was to investigate apoptosis-inducing activity of recombinant MVL (R-MVL) and proteomic changes in A549 cells, and to identify the molecular pathways responsible for the anti-cancer action of R-MVL. We found that R-MVL induces A549 cells apoptosis in a dose-dependent manner by using MTT assay, fluorescent microscope (FM) and flow cytometry (FCM), and the IC50 was calculated to be 24.12 μg/ml. Subsequently, 7 altered proteins in R-MVL-treated A549 cells were identified, including upregulated aldehyde dehydrogenase 1 and β-actin, and five downregulated proteins: heat shock protein 90, heat shock 60, plastin 3, tropomyosin 3, and β-tubulin. Further bioinformatics analysis predicted the potential pathways for R-MVL to induce apoptosis of A549 cells. In conclusion, this is the first report to investigate anti-cancer activity of R-MVL and its mechanism of action by proteomics analysis. Our observations provide potential therapeutic targets for lung cancer inhibitor intervention and implicated the development of novel anti-cancer therapeutic strategies.
College of Light Industry and Food Sciences, South China University of Technology, 381 Wushan Road, Guangzhou, 510640, China.
Yuqin
Y
Li
Bochao
B
Zhang
Xiaoqin
X
Wang
Huidan
H
Yan
Gu
G
Chen
Xuewu
X
Zhang
D000199:Actins / Q000378:metabolism; D000444:Aldehyde Dehydrogenase / Q000378:metabolism; D000080924:Aldehyde Dehydrogenase 1 Family; D000595:Amino Acid Sequence; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects* / Q000235:genetics; D045744:Cell Line, Tumor; D018834:Chaperonin 60 / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D018841:HSP90 Heat-Shock Proteins / Q000378:metabolism; D006801:Humans; D037102:Lectins / Q000494:pharmacology*; D008175:Lung Neoplasms / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology*; D008562:Membrane Glycoproteins / Q000378:metabolism; D046931:Microcystis / Q000737:chemistry; D008840:Microfilament Proteins / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000378:metabolism; D008969:Molecular Sequence Data; D025941:Protein Interaction Mapping; D040901:Proteomics; D011994:Recombinant Proteins / Q000494:pharmacology; D050697:Retinal Dehydrogenase; D014335:Tropomyosin / Q000378:metabolism; D014404:Tubulin / Q000378:metabolism
D000199:Actins; D000970:Antineoplastic Agents; D018834:Chaperonin 60; D018841:HSP90 Heat-Shock Proteins; C060293:HSPD1 protein, human; D037102:Lectins; D008562:Membrane Glycoproteins; D008840:Microfilament Proteins; D024101:Mitochondrial Proteins; D011994:Recombinant Proteins; C498783:TPM3 protein, human; D014335:Tropomyosin; D014404:Tubulin; C026742:plastin; D000080924:Aldehyde Dehydrogenase 1 Family; D000444:Aldehyde Dehydrogenase; C510223:ALDH1A1 protein, human; D050697:Retinal Dehydrogenase