Sleep loss and acute drug abuse can induce DNA damage in multiple organs of mice.
The purpose of the present study was to characterize the genetic damage induced by paradoxical sleep deprivation (PSD) in combination with cocaine or ecstasy (3,4-methylenedioxymethamphetamine; MDMA) in multiple organs of male mice using the single cell gel (comet) assay. C57BL/6J mice were submitted to PSD by the platform technique for 72 hours, followed by drug administration and evaluation of DNA damage in peripheral blood, liver and brain tissues. Cocaine was able to induce genetic damage in the blood, brain and liver cells of sleep-deprived mice at the majority of the doses evaluated. Ecstasy also induced increased DNA migration in peripheral blood cells for all concentrations tested. Analysis of damaged cells by the tail moment data suggests that ecstasy is a genotoxic chemical at the highest concentrations tested, inducing damage in liver or brain cells after sleep deprivation in mice. Taken together, our results suggest that cocaine and ecstasy/MDMA act as potent genotoxins in multiple organs of mice when associated with sleep loss.
Departamento de Psicobiologia, Universidade Federal de São Paulo, São Paulo, Brazil.
T A
TA
Alvarenga
D A
DA
Ribeiro
P
P
Araujo
C
C
Hirotsu
R
R
Mazaro-Costa
J L
JL
Costa
M C
MC
Battisti
S
S
Tufik
M L
ML
Andersen
D019969:Amphetamine-Related Disorders / Q000097:blood / Q000235:genetics*; D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D019970:Cocaine-Related Disorders / Q000097:blood / Q000235:genetics* / Q000473:pathology; D020552:Comet Assay; D004249:DNA Damage; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D012892:Sleep Deprivation / Q000097:blood / Q000235:genetics* / Q000473:pathology