Expression of ventral diencephalon-enriched genes in zebrafish.
Dopaminergic (DA) neurons in the vertebrate di- and mesencephalon play essential roles in movement control, endocrine modulation and many other important physiological activities. To identify genes that may regulate the specification and differentiation of diencephalic DA neurons in zebrafish, the spatial and temporal expression pattern of a set of genes was investigated. In situ hybridization analysis revealed that expression of DNA binding inhibitor 3 (Id3), early B cell factor 2 (Ebf2), Ebf3, Iroquois related homeobox 1 (Irx1), Kruppel-like factor 7 (Klf7), mab-21-like 1 (Mab21l1), fatty acid binding protein 7 (Fabp7) and stathmin-like 4 (Stmn4), were enriched in the diencephalon of zebrafish. Among these genes, Id3 was expressed specifically in a subset of DA neurons in the ventral diencephalon, with co-expression of neurogenin1 (Ngn1). Alteration of expression levels of Id3 inhibited maturation of developing DA neurons. Taken together, our study provides genetic characteristics of DA neurons in the diencephalon of zebrafish.
Institute of Neuroscience, State Key Laboratory of Neuroscience, Shanghai, China.
Sen
S
Li
Min
M
Yin
Shuxi
S
Liu
Yi
Y
Chen
Yanqing
Y
Yin
Tingxi
T
Liu
Jiawei
J
Zhou
D000818:Animals; D004027:Diencephalon / Q000196:embryology / Q000378:metabolism*; D004298:Dopamine / Q000378:metabolism*; D004625:Embryo, Nonmammalian / Q000378:metabolism; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins / Q000235:genetics / Q000378:metabolism; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D017403:In Situ Hybridization; D051796:Inhibitor of Differentiation Proteins / Q000235:genetics / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D015027:Zebrafish; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism*
D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D018398:Homeodomain Proteins; D051796:Inhibitor of Differentiation Proteins; D009419:Nerve Tissue Proteins; D029961:Zebrafish Proteins; C490175:fabp7a protein, zebrafish; C499232:mab21l1 protein, zebrafish; D004298:Dopamine