[Detection of parvovirus B19 markers in blood samples of donors].
OBJECTIVE
To assess detection rate of parvovirus B19 markers in population of donors in one of the regions of Russian Federation.


METHODS
Screening of blood samples from 1000 donors for parvovirus B19 DNA was performed by real-time polymerase chain reaction. Levels of specific antibodies to parvovirus B19 were measured by quantitative enzyme immunoassay (R-Biopharm AG, Germany).


RESULTS
Parvovirus B19 DNA was detected in 10 samples (1%) with viral load ranging from 1.0 x 10(3) to 1.0 x 10(6) genome equivalents per 1 ml. All DNA-positive samples contained IgG, and one sample contained IgM also. IgG were detected in 29.7% of DNA-negative samples. In these samples specific IgM were not detected. Mean level of IgG in DNA-negative and DNA-positive groups was 27.52 IU/ml [95% CI (19.6 -35.4) IU/ml] and 107.30 IU/ml [95% CI (55.7 - 158.9) IU/ml] respectively. Level of IgG in DNA-negative group was significantly lower than in the DNA-positive (p < 0.0001).


CONCLUSIONS
Results of the study provide new and important information for making rational decision about screening of donors' blood for parvovirus B19 markers.
E V
EV
Filatova
N V
NV
Zubkova
N A
NA
Novikova
L N
LN
Golitsina
K V
KV
Kuznetsov
D000914:Antibodies, Viral / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D001782:Blood Donors; D004279:DNA, Viral / Q000097:blood*; D006801:Humans; D007124:Immunoenzyme Techniques; D010322:Parvoviridae Infections / Q000097:blood / Q000517:prevention & control*; D016732:Parvovirus B19, Human / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D012426:Russia; D019562:Viral Load
D000914:Antibodies, Viral; D015415:Biomarkers; D004279:DNA, Viral