Genopal™: a novel hollow fibre array for focused microarray analysis.
Expression profiling of target genes in patient blood is a powerful tool for RNA diagnosis. Here, we describe Genopal™, a novel platform ideal for efficient focused microarray analysis. Genopal™, which consists of gel-filled fibres, is advantageous for high-quality mass production via large-scale slicing of the Genopal™ block. We prepared two arrays, infectant and autoimmunity, that provided highly reliable data in terms of repetitive scanning of the same and/or distinct microarrays. Moreover, we demonstrated that Genopal™ had sensitivity sufficient to yield signals in short hybridization times (0.5 h). Application of the autoimmunity array to blood samples allowed us to identify an expression pattern specific to Takayasu arteritis based on the Spearman rank correlation by comparing the reference profile with those of several autoimmune diseases and healthy volunteers (HVs). The comparison of these data with those obtained by other methods revealed that they exhibited similar expression profiles of many target genes. Taken together, these data demonstrate that Genopal™ is an advantageous platform for focused microarrays with regard to its low cost, rapid results and reliable quality.
Department of Molecular Genetics, Research Institute for Microbial Diseases, Suita, Osaka, Japan.
Daisuke
D
Okuzaki
Tatsunobu
T
Fukushima
Takahiro
T
Tougan
Tomonori
T
Ishii
Shigeto
S
Kobayashi
Kazuyuki
K
Yoshizaki
Takashi
T
Akita
Hiroshi
H
Nojima
D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D021141:Nucleic Acid Amplification Techniques; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012313:RNA / Q000032:analysis*; D018709:Statistics, Nonparametric; D013625:Takayasu Arteritis / Q000235:genetics*
D012313:RNA