Transcriptome and proteome profiling of host responses to Marek's disease virus in chickens.
Marek's disease (MD) is an immunosuppressive and proliferative disease of domestic chickens caused by a highly oncogenic cell-associated alpha-herpesvirus, named Marek's disease virus (MDV). Despite the availability of highly efficacious vaccines for control of MD and existence of lines of chickens which display differential genetic susceptibility or resistance to this disease, little is known about the underlying mechanisms of MDV-host interactions. The recent advent of global or targeted gene and protein expression profiling has paved the way towards gaining a better understanding of host responses to MDV. The main objective of this review is to discuss some of the recent advancements made in relation to elucidating the mechanisms of MDV pathogenesis, host responses to MDV, genetic resistance/susceptibility to MD, and immunity conferred by vaccines. In this regard, particular emphasis has been placed on studies employing proteome and transcriptome profiling approaches. Finally, the utility of microRNA and RNA interference (RNAi) technologies for functional analysis of genes, proteins, and pathways that play a role in the complex interactions between MDV and its host is discussed.
Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada N1G 2W1.
Kamran
K
Haq
Jennifer T
JT
Brisbin
Niroshan
N
Thanthrige-Don
Mohammad
M
Heidari
Shayan
S
Sharif
D000818:Animals; D002645:Chickens; D003199:Computer Systems; D020869:Gene Expression Profiling / Q000662:veterinary; D020022:Genetic Predisposition to Disease; D023281:Genomics; D054884:Host-Pathogen Interactions / Q000235:genetics / Q000276:immunology; D025922:Mardivirus / Q000235:genetics / Q000276:immunology; D008380:Marek Disease / Q000235:genetics* / Q000276:immunology* / Q000517:prevention & control; D022501:Marek Disease Vaccines / Q000494:pharmacology; D035683:MicroRNAs / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000662:veterinary; D020543:Proteome; D040901:Proteomics; D034622:RNA Interference
D022501:Marek Disease Vaccines; D035683:MicroRNAs; D020543:Proteome