A seed coat outer integument-specific promoter for Brassica napus.
In search for seed coat-specific promoters for canola (Brassica napus), transgenic plants carrying a 2,121 bp fragment of Arabidopsis thaliana At4g12960 promoter (AtGILTpro) fused to the uidA reporter gene (GUS) were generated. Out of 7 independent events in transgenic canola plants raised, 2 exhibited GUS activity exclusively in the outer integument of the seed coat. GUS activity in other tissues was also observed in the remaining five transformants. Therefore, the AtGILT promoter can be used as a canola seed coat outer integument-specific promoter after the generation and selection of desired transformants from several transgenic lines.
Alberta Innovates-Technology Futures, PO Bag 4000, Hwy 16A & 75 St., Vegreville, AB, T9C 1T4, Canada. Limin.Wu@albertainnovates.ca
Limin
L
Wu
Aliaa
A
El-Mezawy
Saleh
S
Shah
D017360:Arabidopsis / Q000235:genetics; D029688:Brassica napus / Q000166:cytology / Q000187:drug effects / Q000235:genetics*; D002934:Cinnamates / Q000494:pharmacology; D004352:Drug Resistance, Microbial / Q000187:drug effects / Q000235:genetics; D005966:Glucuronidase / Q000235:genetics / Q000378:metabolism; D006921:Hygromycin B / Q000031:analogs & derivatives / Q000494:pharmacology; D009928:Organ Specificity / Q000187:drug effects / Q000235:genetics; D030821:Plants, Genetically Modified; D054817:Pollination / Q000187:drug effects / Q000235:genetics; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D015203:Reproducibility of Results; D012639:Seeds / Q000187:drug effects / Q000235:genetics*; D014170:Transformation, Genetic / Q000187:drug effects
D002934:Cinnamates; D006921:Hygromycin B; C026273:hygromycin A; D005966:Glucuronidase