Bioavailability of cyanidin glycosides from natural chokeberry (Aronia melanocarpa) juice with dietary-relevant dose of anthocyanins in humans.
The aim of this study was to investigate the bioavailability of anthocyanins from chokeberry juice with a dietary-relevant dose of anthocyanins. Thirteen healthy volunteers consumed chokeberry juice providing 0.8 mg of anthocyanins/kg of body weight. Before and after juice consumption, blood and urine were collected. Concentration of anthocyanins was measured with HPLC-PDA-MS-ESI. Cyanidin-3-galactoside comprised 66% of total chokeberry anthocyanins. Eight cyanidin derivatives were found in blood and urine after juice consumption. The maximum plasma anthocyanin concentration of 32.7 ± 2.9 nmol/L was reached at 1.3 ± 0.1 h after juice consumption. The anthocyanins' urine excretion rate (62.9 ± 5.0 nmol/h) was the highest within the first 2 h. In total, 0.25 ± 0.02% of the ingested anthocyanins was excreted by the renal route during 24 h, mainly as metabolites of cyanidin. According to these observations, after consumption of a dietary-relevant dose of anthocyanins as natural chokeberry juice, anthocyanins and their metabolites were present in plasma and urine of volunteers.
Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences in Olsztyn, Tuwima 10, 10-747 Olsztyn, Poland.
Wieslaw
W
Wiczkowski
Ewa
E
Romaszko
Mariusz K
MK
Piskula
D000328:Adult; D000872:Anthocyanins / Q000737:chemistry / Q000378:metabolism* / Q000652:urine; D001628:Beverages / Q000032:analysis*; D005260:Female; D006027:Glycosides / Q000737:chemistry / Q000378:metabolism* / Q000652:urine; D006801:Humans; D007700:Kinetics; D008297:Male; D046936:Photinia / Q000737:chemistry / Q000378:metabolism*; D010936:Plant Extracts / Q000737:chemistry / Q000378:metabolism* / Q000652:urine; D055815:Young Adult
D000872:Anthocyanins; D006027:Glycosides; D010936:Plant Extracts; C017154:cyanidin