Construction of novel vectors for transformation of Lentinula edodes using a chitin synthase gene promoter.
Novel vectors (pLCHS-hph and pChG-bar) containing expression unit driving hph as a selectable marker gene by chitin synthase gene promoter were constructed for Lentinula edodes transformation. Expression of the hph gene in random selected transformants was confirmed by RT-PCR method. Thus, both vectors are useful for L. edodes transformation.
Iwate Biotechnology Research Center, 22-174-4 Narita, Kitakami, Iwate 024-0003, Japan. tosisato@mail.kitami-it.ac.jp
Toshitsugu
T
Sato
Kumiko
K
Okawa
Tatsuya
T
Hirano
D002687:Chitin Synthase / Q000235:genetics*; D004271:DNA, Fungal / Q000235:genetics; D005822:Genetic Vectors; D011401:Promoter Regions, Genetic; D020942:Shiitake Mushrooms / Q000235:genetics*; D014170:Transformation, Genetic
D004271:DNA, Fungal; D002687:Chitin Synthase