[Experimental study on effect of pre-acupuncture and moxibustion on molecular markers in pre-thrombosis state of rats].
OBJECTIVE
To explore the best method for prevention and treatment of thrombosis and its mechanism.


METHODS
Forty SD rats were randomly divided into a blank group, a model group, an electroacupuncture group and a crude herb moxibustion group. In the electroacupuncture group and the crude herb moxibustion group. "Zusanli" (ST 36), "Sanyinjiao" (SP 6), "Pishu" (BL 20) and "Geshu" (BL 17) were punctured or moxibusted for 2 weeks, then pre-thrombosis model was induced by Adrenalin Hydrochloride and ice water method in the model group, the electroacupuncture group and the crude herb moxibustion group, respectively. Molecular markers in venous blood after the model made in 18 hours were detected.


RESULTS
Act: vaty of tissue-type plasminogen activator (t-PA) and content of alpha-granule membrane protein (GMP-140) decreased and content of nitrogen monoxidum (NO) increased after electroacupuncture or crude herb moxibustion. The levels of t-PA and GMP-140 in the model group were higher than those in the electroacupuncture group, the crude herb moxibustion group and the blank group (all P < 0.05), and the content of NO in the model group was lower than those in the electroacupuncture group, the crude herb moxibustion group and the blank group (all P < 0.05), while there was no significant difference in t- PA, GMP-140 and NO among the crude herb moxibustion group, electroacupuncture group and blank group (all P > 0.05).


CONCLUSIONS
Electroacupuncture and crude herb moxibustion can significantly change the contents of t-PA, GMP-140 and NO and there was no significant difference between the two therapies.
The First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, China.
Xiang-Lai
XL
Niu
Yu
Y
Zhou
Qing
Q
Lin
Yi-Mei
YM
Bao
Xiao-Ping
XP
Song
D015670:Acupuncture Therapy; D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D008297:Male; D009071:Moxibustion; D009569:Nitric Oxide / Q000097:blood*; D019007:P-Selectin / Q000097:blood*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013927:Thrombosis / Q000097:blood / Q000473:pathology / Q000628:therapy*; D010959:Tissue Plasminogen Activator / Q000097:blood*
D019007:P-Selectin; D009569:Nitric Oxide; D010959:Tissue Plasminogen Activator