[Multilocus sequence-typing of Enterococcus faecium fecal isolates].
OBJECTIVE
Genetic characteristics of Enterococcus faecium strains isolated from human intestine in Russia.


METHODS
Seven strains of E. faecium with antimicrobial activity against Gram-positive bacteria and yeast fungi were isolated from persons aged 4 months - 44 years. Using multilocus sequence-typing, sequences of internal fragments of genes of general metabolism (adk, atpA, ddl, gyd, gdh, purK, pstS) were determined.


RESULTS
Number of alleles for each gene varied from 3 for gdh and pstS to 7 for atpA. Sequence-types of 4 out of 7 cultures of enterococci were described earlier, 3 strains were attributed to new sequence-types.


CONCLUSIONS
Members of identified in this study sequence-types 32, 135, 170, 361 were isolated earlier in other countries from clinical samples (blood, faeces) and hospital environment. Diversity of sequence-types, sources of isolation and significant remoteness of regions where strains belonging to one sequence-type were isolated point to necessity of thorough study of E. faecium evolution.
O V
OV
Bukharin
A V
AV
Valyshev
I V
IV
Valysheva
S A
SA
Ermolaeva
D000293:Adolescent; D000328:Adult; D000483:Alleles; D000898:Antibiosis; D015373:Bacterial Typing Techniques; D002648:Child; D002675:Child, Preschool; D016984:Enterococcus faecium / Q000145:classification* / Q000235:genetics / Q000502:physiology; D005243:Feces / Q000382:microbiology; D005260:Female; D005798:Genes, Bacterial / Q000235:genetics; D006094:Gram-Positive Bacteria / Q000502:physiology; D006801:Humans; D007422:Intestines / Q000382:microbiology*; D008297:Male; D058885:Multilocus Sequence Typing; D012426:Russia; D015003:Yeasts / Q000502:physiology