Effect of low pH on the morphology and viability of Cryptosporidium andersoni sporozoites and histopathology in the stomachs of infected mice.
The genus Cryptosporidium includes many common parasites infecting animals and humans, and is a major cause of diarrheal illness worldwide. The biology of gastric Cryptosporidium spp., including replication in the stomach, has not been well documented. This study evaluated the viability of Cryptosporidium andersoni sporozoites in gastric environments after excystation and examined the endogenous development and histopathological changes in the stomachs of infected mice, using a novel type of C. andersoni. Sporozoites were affected by low pH (61.6% viability after 3h at pH2.0). Electron microscopy revealed developmental parasites on the gastric foveolae but not on the surface of the gastric mucosa. Histopathological examinations at 1, 2, 4 and 12 weeks p.i. uncovered three different lesions. The gastric mucosa of foveolae filled with parasites was extended and the amount of neutral mucopolysaccharide at the mucosal surface was decreased with the first type of lesion. The gastric mucosa was atrophied, some gastric glands were disrupted and the amount of acid mucopolysaccharide at the mucosal surface was increased with the second type. Finally, the gastric mucosa was slightly extended and goblet cells were present in the gastric mucosa, indicating intestinal metaplasia, in the third type. No parasites were detected in these areas with increased acidic mucin and indications of metaplasia. The results suggest that C. andersoni parasites could not survive in acidic environments for a long period before invading host cells and preferentially develop in neutral sites of the gastric mucosa, resulting in histopathological changes and chronic shedding of oocysts.
National Institute of Animal Health, National Agriculture and Food Research Organization, Tsukuba, Ibaraki 305-0856, Japan.
Makoto
M
Matsubayashi
Haruka
H
Ando
Isao
I
Kimata
Hiroshi
H
Takase
Hideki
H
Nakagawa
Masaru
M
Furuya
Hiroyuki
H
Tani
Kazumi
K
Sasai
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000469:parasitology; D003457:Cryptosporidiosis / Q000469:parasitology / Q000473:pathology*; D003458:Cryptosporidium / Q000254:growth & development / Q000648:ultrastructure; D005243:Feces / Q000469:parasitology; D005260:Female; D005753:Gastric Mucosa / Q000469:parasitology / Q000473:pathology; D006863:Hydrogen-Ion Concentration; D051379:Mice; D016513:Mice, SCID; D008854:Microscopy, Electron; D033661:Oocysts / Q000502:physiology; D034101:Sporozoites / Q000254:growth & development / Q000648:ultrastructure; D013270:Stomach / Q000469:parasitology / Q000473:pathology*