Cytotoxic effects of two organotin compounds and their mode of inflicting cell death on four mammalian cancer cells.
In this report, we have tested the cytotoxicity of two organotin (OT) compounds by flow cytometry on a panel of immortalized cancer cell lines of human and murine origin. Although the OT compounds exhibited varying levels of cytotoxicity, diphenylmethyltin chloride was more toxic than 1,4-bis (diphenylchlorostannyl)p-xylene on all cell lines tested. The OT compounds were found to be highly cytotoxic to lymphoma cell lines with lower toxicity toward the HeLa cervical cancer cell line. In order to discern the mechanism by which cell death was induced, additional experiments were conducted to monitor characteristic changes consistent with apoptosis and/or necrosis. Cell lines treated with the experimental compounds indicated that there was no consistent mode of cell death induction. However, both compounds induced apoptosis in the pro-B lymphocyte cell line, NFS-70. The work presented here also demonstrates that the two OT compounds possess selective cytotoxicity against distinct transformed cell lines.
Department of Biological Sciences, Biosciences Research Building, University of Texas at El Paso, El Paso, TX 79968-5816, USA. avarela2@utep.edu
Armando
A
Varela-Ramirez
Margaret
M
Costanzo
Yazmin P
YP
Carrasco
Keith H
KH
Pannell
Renato J
RJ
Aguilera
D000818:Animals; D017304:Annexin A5 / Q000378:metabolism; D000970:Antineoplastic Agents / Q000494:pharmacology* / Q000633:toxicity; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D004354:Drug Screening Assays, Antitumor; D005434:Flow Cytometry; D006801:Humans; D020128:Inhibitory Concentration 50; D007364:Intercalating Agents; D051379:Mice; D009336:Necrosis; D009947:Organotin Compounds / Q000494:pharmacology* / Q000633:toxicity; D011419:Propidium; D013329:Structure-Activity Relationship
C560269:1,4-bis(diphenylchlorostannyl)-p-xylene; D017304:Annexin A5; D000970:Antineoplastic Agents; D007364:Intercalating Agents; D009947:Organotin Compounds; C560268:diphenylmethyltin chloride; D011419:Propidium