Why does insect RNA look degraded?
The integrity of extracted ribonucleic acid (RNA) is commonly assessed by gel electrophoresis and subsequent analysis of the ribosomal RNA (rRNA) bands. Using the honey bee, Apis mellifera (Hymenoptera: Apidae), as an example, the electrophoretic rRNA profile of insects is explained. This profile differs significantly from the standard benchmark since the 28S rRNA of most insects contains an endogenous "hidden break." Upon denaturation, the masking hydrogen bonds are disrupted, releasing two similar sized fragments that both migrate closely with 18S rRNA. The resulting rRNA profile thus reflects the endogenous composition of insect rRNA and should not be misinterpreted as degradation.
Department of Anaesthesiology, Faculty of Medical and Health Sciences, The University of Auckland, Auckland, New Zealand. e.winnebeck@auckland.ac.nz
Eva C
EC
Winnebeck
Craig D
CD
Millar
Guy R
GR
Warman
D000818:Animals; D001516:Bees / Q000235:genetics*; D004586:Electrophoresis / Q000592:standards; D006860:Hydrogen Bonding; D009690:Nucleic Acid Conformation; D012335:RNA, Ribosomal / Q000737:chemistry*
D012335:RNA, Ribosomal