Uncovering new substrates for Aurora A kinase.
Aurora A is a serine/threonine kinase that is essential for a wide variety of cell-cycle-related events, but only a small number of its substrates are known. We present and validate a strategy by which to identify Aurora A substrates and their phosphorylation sites. We developed a computational approach integrating various types of biological information to generate a list of 90 potential Aurora substrates, with a prediction accuracy of about 80%. We also demonstrated the specific phosphorylation of NUSAP (nucleolar and spindle-associated protein) by Aurora A in vivo. Our results provide a means by which to develop an understanding of Aurora A function and suggest unexpected roles for this kinase.
Department of Cell and Developmental Biology, Centre for Genomic Regulation, Barcelona, Spain.
Teresa
T
Sardon
Roland A
RA
Pache
Amelie
A
Stein
Henrik
H
Molina
Isabelle
I
Vernos
Patrick
P
Aloy
D000595:Amino Acid Sequence; D064127:Aurora Kinases; D019295:Computational Biology; D006801:Humans; D008869:Microtubule-Associated Proteins / Q000737:chemistry / Q000378:metabolism; D008938:Mitosis; D008954:Models, Biological; D008969:Molecular Sequence Data; D010766:Phosphorylation; D010768:Phosphoserine / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D015203:Reproducibility of Results; D013379:Substrate Specificity
D008869:Microtubule-Associated Proteins; C000709213:NUSAP1 protein, human; D010768:Phosphoserine; D064127:Aurora Kinases; D017346:Protein Serine-Threonine Kinases