Nanomechanical properties of biochemically modified dentin bonded interfaces.
The effect of biomodification of dentin matrices using collagen cross-linkers, glutaraldehyde (GD) and grape seed extract (GSE), on the reduced modulus of elasticity (Er) and nanohardness (H) of the hybrid layer and underlying dentin was investigated at the dentin-resin bonded interface. The coronal dentin of nine molars were exposed and divided into groups: 5% GD, 6·5% GSE and control. Control samples were etched, bonded with Adper Single Bond Plus and Premise composite. GD and GSE were applied for 1 h prior to bonding procedures. After 24 h, samples were sectioned, and resin-dentin beams were either kept in distilled water or exposed to collagenase treatment for 24 h. Nano-indentations were performed at the hybrid layer and underlying dentin. GD and GSE treatment increased the Er and H of resin-dentin interface structures when compared to the control group (P<0·05), particularly the hybrid layer, and may be a promising novel approach to strengthen the dentin-resin bonded interface structures when using these adhesive system and resin-based composite.
Department of Dental Materials and Prosthodontics, Araçatuba School of Dentistry, São Paulo State University-UNESP, Araçatuba, SP, Brazil. paulosantos@foa.unesp.br
P H
PH
dos Santos
S
S
Karol
A K B
AK
Bedran-Russo
D000268:Adhesiveness; D003188:Composite Resins / Q000138:chemical synthesis*; D001840:Dental Bonding / Q000379:methods*; D003804:Dentin; D017220:Dentin-Bonding Agents / Q000737:chemistry*; D004548:Elasticity; D005976:Glutaral / Q000138:chemical synthesis*; D056604:Grape Seed Extract / Q000138:chemical synthesis*; D006801:Humans; D008422:Materials Testing; D036103:Nanotechnology / Q000379:methods; D013499:Surface Properties
D003188:Composite Resins; D017220:Dentin-Bonding Agents; D056604:Grape Seed Extract; D005976:Glutaral