Effect of Ambrotose AO® on resting and exercise-induced antioxidant capacity and oxidative stress in healthy adults.
BACKGROUND
The purpose of this investigation was to determine the effects of a dietary supplement (Ambrotose AO®) on resting and exercise-induced blood antioxidant capacity and oxidative stress in exercise-trained and untrained men and women.


METHODS
25 individuals (7 trained and 5 untrained men; 7 trained and 6 untrained women) received Ambrotose AO® (4 capsules per day = 2 grams per day) or a placebo for 3 weeks in a random order, double blind cross-over design (with a 3 week washout period). Blood samples were collected at rest, and at 0 and 30 minutes following a graded exercise treadmill test (GXT) performed to exhaustion, both before and after each 3 week supplementation period. Samples were analyzed for Trolox Equivalent Antioxidant Capacity (TEAC), Oxygen Radical Absorbance Capacity (ORAC), malondialdehyde (MDA), hydrogen peroxide (H2O2), and nitrate/nitrite (NOx). Quality of life was assessed using the SF-12 form and exercise time to exhaustion was recorded. Resting blood samples were analyzed for complete blood count (CBC), metabolic panel, and lipid panel before and after each 3 week supplementation period. Dietary intake during the week before each exercise test was recorded.


RESULTS
No condition effects were noted for SF-12 data, for GXT time to exhaustion, or for any variable within the CBC, metabolic panel, or lipid panel (p > 0.05). Treatment with Ambrotose AO® resulted in an increase in resting levels of TEAC (p = 0.02) and ORAC (p < 0.0001). No significant change was noted in resting levels of MDA, H2O2, or NOx (p > 0.05). Exercise resulted in an acute increase in TEAC, MDA, and H2O2 (p < 0.05), all which were higher at 0 minutes post exercise compared to pre exercise (p < 0.05). No condition effects were noted for exercise related data (p > 0.05), with the exception of ORAC (p = 0.0005) which was greater at 30 minutes post exercise for Ambrotose AO® compared to placebo.


CONCLUSIONS
Ambrotose AO® at a daily dosage of 4 capsules per day increases resting blood antioxidant capacity and may enhance post exercise antioxidant capacity. However, no statistically detected difference is observed in resting or exercise-induced oxidative stress biomarkers, in quality of life, or in GXT time to exhaustion.
Cardiorespiratory/Metabolic Laboratory, The University of Memphis, Memphis, TN 38152, USA. rbloomer@memphis.edu
Richard J
RJ
Bloomer
Robert E
RE
Canale
Megan M
MM
Blankenship
Kelsey H
KH
Fisher-Wellman
D000328:Adult; D000975:Antioxidants / Q000008:administration & dosage / Q000378:metabolism*; D015415:Biomarkers / Q000032:analysis; D019587:Dietary Supplements; D005080:Exercise Test; D005260:Female; D006801:Humans; D006861:Hydrogen Peroxide / Q000097:blood; D008055:Lipids / Q000097:blood; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008875:Middle Aged; D009589:Nitrogen Oxides / Q000097:blood; D018384:Oxidative Stress / Q000187:drug effects*; D010349:Patient Compliance
D000975:Antioxidants; D015415:Biomarkers; D008055:Lipids; D009589:Nitrogen Oxides; D008315:Malondialdehyde; D006861:Hydrogen Peroxide