Microbial production of xylitol from D-xylose and sugarcane bagasse hemicellulose using newly isolated thermotolerant yeast Debaryomyces hansenii.
A thermotolerant yeast capable of fermenting xylose to xylitol at 40°C was isolated and identified as a strain of Debaryomyces hansenii by ITS sequencing. This paper reports the production of xylitol from D-xylose and sugarcane bagasse hemicellulose by free and Ca-alginate immobilized cells of D. hansenii. The efficiency of free and immobilized cells were compared for xylitol production from D-xylose and hemicellulose in batch culture at 40°C. The maximum xylitol produced by free cells was 68.6 g/L from 100 g/L of xylose, with a yield of 0.76 g/g and volumetric productivity 0.44 g/L/h. The yield of xylitol and volumetric productivity were 0.69 g/g and 0.28 g/L/h respectively from hemicellulosic hydrolysate of sugarcane bagasse after detoxification with activated charcoal and ion exchange resins. The Ca-alginate immobilized D. hansenii cells produced 73.8 g of xylitol from 100 g/L of xylose with a yield of 0.82 g/g and volumetric productivity of 0.46 g/L/h and were reused for five batches with steady bioconversion rates and yields.
Biochemical Sciences Division, National Chemical Laboratory, Pune, India.
Gyan
G
Prakash
A J
AJ
Varma
Asmita
A
Prabhune
Yogesh
Y
Shouche
Mala
M
Rao
D002482:Cellulose / Q000378:metabolism*; D011134:Polysaccharides / Q000378:metabolism*; D004718:Saccharomycetales / Q000378:metabolism*; D031786:Saccharum / Q000382:microbiology*; D014993:Xylitol / Q000096:biosynthesis*; D014994:Xylose / Q000096:biosynthesis*
D011134:Polysaccharides; C007916:hemicellulose; D002482:Cellulose; C027433:bagasse; D014994:Xylose; D014993:Xylitol