Coffea arabica extract and its constituents prevent photoaging by suppressing MMPs expression and MAP kinase pathway.
UV is a potent factor in skin photoaging and photocarcinogenesis. Therefore, investigating the inhibiting mechanisms of photoaging would be useful to enable development of agents to slow down the aging process. UV-irradiation increased metalloproteinase (MMP)-1, -3, and -9 and then causes collagen and elastin degradation, leading to the formation of coarse wrinkles and sagging skin. Polyphenols, a group of compounds, possessing a variety of biological activities including inhibition of MMP-1 and elastase, are widely distributed in plants including Coffea arabica. In this study, Coffea arabica leaves extract (CAE), its hydrolysates (CAH), chlororgenic acid and caffeic acid, are studied for their anti-photoaging effect. Coffea arabica leaves were extracted with methanol, and the extract was hydrolyzed with different concentrations of hydrochloric acid. The various concentrations of CAE, CAH, chlororgenic acid and caffeic acid were subject to MMPs and elastase inhibition tests. The fibroblast was used for collagen synthesis and MMP-1, -3, -9 inhibition tests on herbal extracts. The results showed that CAE stimulated type I procollagen expression, inhibited MMP-1, -3, -9 expression and inhibited the phosphorylation of JNK, ERK and p38. The results suggest that CAE can prevent photo-damage in skin through inhibiting MMP expression and MAP kinase pathway.
Department of Cosmeceutics, China Medical University, Taichung, Taiwan.
Hsiu-Mei
HM
Chiang
Tsen-Jung
TJ
Lin
Chen-Yuan
CY
Chiu
Chiung-Wen
CW
Chang
Kuo-Chiu
KC
Hsu
Pei-Ching
PC
Fan
Kuo-Ching
KC
Wen
D000975:Antioxidants / Q000494:pharmacology; D002478:Cells, Cultured; D040503:Coffea / Q000737:chemistry*; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D020935:MAP Kinase Signaling System / Q000187:drug effects*; D061965:Matrix Metalloproteinase Inhibitors; D010196:Pancreatic Elastase / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D011480:Protease Inhibitors / Q000494:pharmacology; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D015595:Skin Aging / Q000187:drug effects*; D014466:Ultraviolet Rays
D000975:Antioxidants; D061965:Matrix Metalloproteinase Inhibitors; D010936:Plant Extracts; D011480:Protease Inhibitors; D047428:Protein Kinase Inhibitors; D010196:Pancreatic Elastase