[Study of early pathological changes and axonal injury of optic nerve in experimental allergic encephalomyelitis mouse].
OBJECTIVE
To observe the early pathological changes and myelin axonal injury of optic nerve during the different stage in EAE mouse, which can provide the theoretical evidence for further therapy.


METHODS
It was an experimental study. EAE was induced with synthetic peptides that represent aa 35 to 55 (MOG35-55) by immunizing C57BL/6 mouse; To observe the pathological change of optic nerve in normal group, EAE group post-immunization 7, 11, 15, 19 d by morphology; The expression of β-APP proteins marked axonal injury and normal myelin proteins CNPase in normal group, EAE group optic nerve after immunization 7, 11, 15, 19 d were detected by immunohistochemistry.


RESULTS
More and more serious inflammation occurred in the optic nerve of EAE group after immunization 7, 11, 15, 19 d; The expression of CNPase proteins in the optic nerve of EAE group decreased post-immunization 11 d, so are in 15 d, 19 d, and the expression of β-APP proteins in the optic nerve of EAE group increased significantly from 7 d to 19 d post-immunization.


CONCLUSIONS
Inflammation in optic nerve of EAE mouse differ from early stage to peak stage after EAE induction; Axonal damage existing in the optic nerve of EAE mouse is independent on demyelination.
Department of Ophthalmology, The First Affiliated Hospital, China Medical University, Shenyang, China.
Rong-wei
RW
Zhang
Lei
L
Chen
D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases / Q000378:metabolism; D016564:Amyloid beta-Protein Precursor / Q000378:metabolism; D000818:Animals; D001369:Axons / Q000473:pathology*; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000473:pathology*; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D009900:Optic Nerve / Q000473:pathology*
D016564:Amyloid beta-Protein Precursor; D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases