[Expression and characterization of rabies virus nucleoprotein in baculovirus].
To construct a recombinant expression plasmid Bacmid-N containing the N gene of Rabies Virus, the N gene of RV CVS-11 strain was cloned into the baculovirus shuttle vector (Bacmid). Recombinant Baculovirus AcMNPV-N (P1 Viral stock) was obtained by transfecting the Bacmid-N into the insect cell line of Sf9. The expressed nucleoprotein was identified and analysised by ELISA, FA, SDS-PAGE and Western blot assays. The results showed that the NP protein was expressed intracellularly and had a good antigenicity, which would be potentially used for further study on the diagnostic reagent of rabies virus detection.
State Key Laboratory for Molecular Virology and Genetic Engineering, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention (IVDC, China CDC) Beijing 100052, China.
Lei
L
Cao
Qing
Q
Tang
Xiao-yan
XY
Tao
Ying
Y
Huang
Jia-liang
JL
Du
Shou-feng
SF
Zhang
Rong-liang
RL
Hu
D000818:Animals; D016367:Baculoviridae / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D004279:DNA, Viral / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D019084:Fluorescent Antibody Technique, Indirect; D009698:Nucleoproteins / Q000235:genetics / Q000378:metabolism*; D011820:Rabies virus / Q000235:genetics* / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018411:Spodoptera
D004279:DNA, Viral; D009698:Nucleoproteins