Human endothelial and osteoblast co-cultures on 3D biomaterials.
Increasingly, in vitro experiments are being used to evaluate the cell compatibility of novel biomaterials. Single cell cultures have been used to determine how well cells attach, grow, and exhibit characteristic functions on these materials and the outcome of such tests is generally accepted as an indicator of biocompatibility. However, organs and tissues are not made up of one cell type and the interaction of cells is known to be an essential factor for physiological cell function. To more accurately examine biomaterials for bone regeneration, we have developed methods to coculture osteoblasts, which are the primary cell type making up bone, and endothelial cells, which form the vasculature supplying cells in the bone with oxygen and nutrients to survive on 2- and 3-D biomaterials.
Institute of Pathology, Johannes Gutenberg University, Mainz, Germany.
Ronald E
RE
Unger
Sven
S
Halstenberg
Anne
A
Sartoris
C James
CJ
Kirkpatrick
D001672:Biocompatible Materials / Q000494:pharmacology*; D002452:Cell Count; D002469:Cell Separation; D002478:Cells, Cultured; D018920:Coculture Techniques / Q000379:methods*; D020405:Dermis / Q000166:cytology; D042783:Endothelial Cells / Q000166:cytology* / Q000187:drug effects*; D006801:Humans; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D055806:Microvessels / Q000166:cytology; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects*; D045663:Quantum Dots; D013194:Staining and Labeling; D013997:Time Factors
D001672:Biocompatible Materials