[Effect of Qingchang Suppository on intestinal permeability in rats with ulcerative colitis].
OBJECTIVE
To observe the effect of Qingchang Suppository (QCS, a Chinese herbal preparation) on intestinal permeability in rat ulcerative colitis (UC) model induced by trinitrobenzene sulforic acid, and to explore the mechanism of QCS for healing the ulceration.


METHODS
Labelled by FITC-dextran 4 000 (FD-4), the permeability of colonic membrane in UC rat and effect of QCS on it were observed in vitro and in vivo.


RESULTS
In vivo study showed that the colonic FD-4 permeability of UC rat was increased significantly, being 6-fold of normal in 30 min. After treated with QCS of high/moderate dosage, it significantly attenuated to different degrees (P < 0.05). FD-4 permeability coefficient (Papp) determination in vitro showed that Papp in model rats increased to (5.001 +/- 1.316) x10(-8) cm/s in 120 min, being 2.5-fold of control; and which could be decreased by high/moderate dose QCS effectively (P < 0.05).


CONCLUSIONS
QCS could suppress the high colonic permeability in UC model rats, improve the barrier function of intestinal membrane and promote the healing of ulceration. Qingchang Suppository; ulcerative colitis; intestinal permeability in UC model rats, improve the barrier function of intestinal membrane and promote the healing of ulceration.
Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai.
Lu
L
Lu
Jian-Qun
JQ
Xie
D000818:Animals; D002463:Cell Membrane Permeability / Q000187:drug effects*; D003093:Colitis, Ulcerative / Q000139:chemically induced / Q000188:drug therapy* / Q000503:physiopathology; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage* / Q000494:pharmacology; D007413:Intestinal Mucosa / Q000502:physiology; D008297:Male; D008517:Phytotherapy; D051381:Rats; D017207:Rats, Sprague-Dawley; D013488:Suppositories; D014302:Trinitrobenzenesulfonic Acid
D004365:Drugs, Chinese Herbal; D013488:Suppositories; D014302:Trinitrobenzenesulfonic Acid