Crystallization, preliminary X-ray diffraction studies and Raman microscopy of the major haemoglobin from the sub-Antarctic fish Eleginops maclovinus in the carbomonoxy form.
The blood of the sub-Antarctic fish Eleginops maclovinus (Em) contains three haemoglobins. The major haemoglobin (Hb1Em) displays the Root effect, a drastic decrease in the oxygen affinity and a loss of cooperativity at acidic pH. The carbomonoxy form of HbEm1 has been crystallized in two different crystal forms, orthorhombic (Ortho) and hexagonal (Hexa), and high-resolution diffraction data have been collected for both forms (1.45 and 1.49 Å resolution, respectively). The high-frequency resonance Raman spectra collected from the two crystal forms using excitation at 514 nm were almost indistinguishable. Hb1Em is the first sub-Antarctic fish Hb to be crystallized and its structural characterization will shed light on the molecular mechanisms of cold adaptation and the role of the Root effect in fish haemoglobins.
Department of Chemistry, University of Naples Federico II, 80126 Naples, Italy.
Antonello
A
Merlino
Luigi
L
Vitagliano
Anna
A
Balsamo
Francesco P
FP
Nicoletti
Barry D
BD
Howes
Daniela
D
Giordano
Daniela
D
Coppola
Guido
G
di Prisco
Cinzia
C
Verde
Giulietta
G
Smulevich
Lelio
L
Mazzarella
Alessandro
A
Vergara
D000818:Animals; D002263:Carboxyhemoglobin / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D010473:Perciformes; D013059:Spectrum Analysis, Raman
D002263:Carboxyhemoglobin