Negative association of Epstein-Barr virus or herpes simplex virus-1 with tumefactive central nervous system inflammatory demyelinating disease.
Central nervous system (CNS) demyelination has been suggested to be associated with infections caused by the Epstein-Barr virus (EBV) or herpes simplex virus (HSV)-1. CNS inflammatory demyelinating disease (IDD) rarely presents as a large lesion. We evaluated samples of serum and cerebrospinal fluid (CSF) by enzyme-linked immunosorbent assay to detect recent infection with these viruses and analyzed CSF and brain specimens by polymerase chain reaction (PCR) or immunohistochemical studies for evidence of these viruses in three patients with biopsy-proven CNS IDD. The results of PCR tests for EBV and HSV in CSF or brain specimens were negative. Elevated anti-EBV or -HSV antibody levels were not found in serum or CSF in any patient. Immunohistochemical studies showed that IDD lesions were negative for latent membrane protein (LMP)-1, Epstein-Barr nuclear antigen (EBNA)-2, and EBNA noncoding RNA (EBER)-1. These results suggest a negative association between CNS IDD and EBV or HSV.
Department of Neurology, Nara Medical University, Kashihara, Nara, Japan.
Takao
T
Kiriyama
Hiroshi
H
Kataoka
Takahiko
T
Kasai
Akitaka
A
Nonomura
Satoshi
S
Ueno
D000328:Adult; D000914:Antibodies, Viral / Q000134:cerebrospinal fluid; D002490:Central Nervous System / Q000473:pathology / Q000821:virology*; D020805:Central Nervous System Viral Diseases / Q000134:cerebrospinal fluid / Q000150:complications* / Q000378:metabolism; D004279:DNA, Viral / Q000134:cerebrospinal fluid; D020031:Epstein-Barr Virus Infections / Q000150:complications*; D005260:Female; D020275:Guillain-Barre Syndrome / Q000134:cerebrospinal fluid / Q000150:complications* / Q000821:virology; D006561:Herpes Simplex / Q000134:cerebrospinal fluid / Q000150:complications*; D018259:Herpesvirus 1, Human / Q000472:pathogenicity; D004854:Herpesvirus 4, Human / Q000472:pathogenicity; D006801:Humans; D008297:Male; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000134:cerebrospinal fluid / Q000821:virology; D016133:Polymerase Chain Reaction
D000914:Antibodies, Viral; D004279:DNA, Viral