DEAD-box protein facilitated RNA folding in vivo.
In yeast mitochondria the DEAD-box helicase Mss116p is essential for respiratory growth by acting as group I and group II intron splicing factor. Here we provide the first structure-based insights into how Mss116p assists RNA folding in vivo. Employing an in vivo chemical probing technique, we mapped the structure of the ai5γ group II intron in different genetic backgrounds to characterize its intracellular fold. While the intron adopts the native conformation in the wt yeast strain, we found that the intron is able to form most of its secondary structure, but lacks its tertiary fold in the absence of Mss116p. This suggests that ai5γ is largely unfolded in the mss116-knockout strain and requires the protein at an early step of folding. Notably, in this unfolded state misfolded substructures have not been observed. As most of the protein-induced conformational changes are located within domain D1, Mss116p appears to facilitate the formation of this largest domain, which is the scaffold for docking of other intron domains. These findings suggest that Mss116p assists the ordered assembly of the ai5γ intron in vivo.
Department of Biochemistry and Cell Biology, University of Vienna, Vienna, Austria.
Andreas
A
Liebeg
Oliver
O
Mayer
Christina
C
Waldsich
D020134:Catalytic Domain; D053487:DEAD-box RNA Helicases / Q000378:metabolism*; D007438:Introns / Q000235:genetics; D008928:Mitochondria / Q000235:genetics / Q000378:metabolism; D009690:Nucleic Acid Conformation; D012313:RNA / Q000737:chemistry / Q000378:metabolism*; D015003:Yeasts / Q000201:enzymology* / Q000235:genetics*
D012313:RNA; D053487:DEAD-box RNA Helicases