Molecular defect of isovaleryl-CoA dehydrogenase in the skunk mutant of silkworm, Bombyx mori.
The isovaleric acid-emanating silkworm mutant skunk (sku) was first studied over 30 years ago because of its unusual odour and prepupal lethality. Here, we report the identification and characterization of the gene responsible for the sku mutant. Because of its specific features and symptoms similar to human isovaleryl-CoA dehydrogenase (IVD) deficiency, also known as isovaleric acidaemia, IVD dysfunction in silkworms was predicted to be responsible for the phenotype of the sku mutant. Linkage analysis revealed that the silkworm IVD gene (BmIVD) was closely linked to the odorous phenotype as expected, and a single amino acid substitution (G376V) was found in BmIVD of the sku mutant. To investigate the effect of the G376V substitution on BmIVD function, wild-type and sku-type recombinants were constructed with a baculovirus expression system and the subsequent enzyme activity of sku-type BmIVD was shown to be significantly reduced compared with that of wild-type BmIVD. Molecular modelling suggested that this reduction in the enzyme activity may be due to negative effects of G376V mutation on FAD-binding or on monomer-monomer interactions. These observations strongly suggest that BmIVD is responsible for the sku locus and that the molecular defect in BmIVD causes the characteristic smell and prepupal lethality of the sku mutant. To our knowledge, this is, aside from humans, the first characterization of IVD deficiency in metazoa. Considering that IVD acts in the third step of leucine degradation and the sku mutant accumulates branched-chain amino acids in haemolymph, this mutant may be useful in the investigation of unique branched-chain amino acid catabolism in insects.
Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, University of Tokyo, Tokyo, Japan.
Kei
K
Urano
Takaaki
T
Daimon
Yutaka
Y
Banno
Kazuei
K
Mita
Tohru
T
Terada
Kentaro
K
Shimizu
Susumu
S
Katsuma
Toru
T
Shimada
D000592:Amino Acid Metabolism, Inborn Errors / Q000235:genetics; D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000818:Animals; D015152:Blotting, Northern; D012831:Bombyx / Q000201:enzymology / Q000235:genetics*; D003001:Cloning, Molecular; D057075:Enzyme Assays; D020869:Gene Expression Profiling; D045782:Hemiterpenes; D006801:Humans; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D050769:Isovaleryl-CoA Dehydrogenase / Q000172:deficiency / Q000235:genetics* / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D050505:Mutant Proteins / Q000378:metabolism; D009154:Mutation; D009812:Odorants; D010421:Pentanoic Acids / Q000378:metabolism; D010641:Phenotype; D011487:Protein Conformation; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity
D045782:Hemiterpenes; D019476:Insect Proteins; D050505:Mutant Proteins; D010421:Pentanoic Acids; C008216:isovaleric acid; D050769:Isovaleryl-CoA Dehydrogenase