Genetic mapping and developmental timing of transmission ratio distortion in a mouse interspecific backcross.
BACKGROUND
Transmission ratio distortion (TRD), defined as statistically significant deviation from expected 1:1 Mendelian ratios of allele inheritance, results in a reduction of the expected progeny of a given genotype. Since TRD is a common occurrence within interspecific crosses, a mouse interspecific backcross was used to genetically map regions showing TRD, and a developmental analysis was performed to identify the timing of allele loss.


RESULTS
Three independent events of statistically significant deviation from the expected 50:50 Mendelian inheritance ratios were observed in an interspecific backcross between the Mus musculus A/J and the Mus spretus SPRET/EiJ inbred strains. At weaning M. musculus alleles are preferentially inherited on Chromosome (Chr) 7, while M. spretus alleles are preferentially inherited on Chrs 10 and 11. Furthermore, alleles on Chr 3 modify the TRD on Chr 11. All TRD loci detected at weaning were present in Mendelian ratios at mid-gestation and at birth.


CONCLUSIONS
Given that Mendelian ratios of inheritance are observed for Chr 7, 10 and 11 during development and at birth, the underlying causes for the interspecific TRD events are the differential post-natal survival of pups with specific genotypes. These results are consistent with the TRD mechanism being deviation from Mendelian inheritance rather than meiotic drive or segregation distortion.
Department of Genetics, Curriculum in Genetics and Molecular Biology, and Carolina Center for Genome Sciences, University of North Carolina, Chapel Hill, NC 27599, USA.
Chevonne D
CD
Eversley
Tavia
T
Clark
Yuying
Y
Xie
Jill
J
Steigerwalt
Timothy A
TA
Bell
Fernando P M
FP
de Villena
David W
DW
Threadgill
D000483:Alleles; D000818:Animals; D002874:Chromosome Mapping; D005260:Female; D005838:Genotype; D006824:Hybridization, Genetic; D040582:Inheritance Patterns; D008297:Male; D051379:Mice / Q000235:genetics*; D008805:Mice, Inbred A; D018895:Microsatellite Repeats; D008957:Models, Genetic