Production of aglycon protopanaxadiol via compound K by a thermostable β-glycosidase from Pyrococcus furiosus.
The production of compound K and aglycon protopanaxadiol (APPD) from ginsenoside Rd and ginseng root extract was performed using a recombinant β-glycosidase from Pyrococcus furiosus. The activity for Rd was optimal at pH 5.5 and 95°C with a half-life of 68 h at 95°C. β-Glycosidase converted Rb(1), Rb(2), Rc, and Rd to APPD via compound K. With increases in the enzyme activity, the productivities of compound K and APPD increased. The substrate concentration was optimal at 4.0 mM Rd or 10% (w/v) ginseng root extract; 4 mM of Rd was converted to 3.3 mM compound K with a yield of 82.5% (mol/mol) and a productivity of 2,010 mg l(-1) h(-1) at 1 h and was hydrolyzed completely to APPD with 364 mg l(-1) h(-1) after 5 h. Rb(1), Rb(2), Rc, and Rd at 3.9 mM in 10% ginseng root extract were converted to 3.1 mM compound K with 79.5% and 1,610 mg l(-1) h(-1) at 1.2 h and were hydrolyzed completely to APPD with 300 mg l(-1) h(-1) after 6 h. The concentrations and productivities of compound K and APPD in the present study are the highest ever reported.
Department of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, Republic of Korea.
Mi-Hyun
MH
Yoo
Soo-Jin
SJ
Yeom
Chang-Su
CS
Park
Ki-Won
KW
Lee
Deok-Kun
DK
Oh
D004795:Enzyme Stability; D036145:Ginsenosides / Q000378:metabolism*; D006207:Half-Life; D006863:Hydrogen-Ion Concentration; D005894:Panax / Q000737:chemistry; D010936:Plant Extracts / Q000302:isolation & purification / Q000378:metabolism*; D018517:Plant Roots / Q000737:chemistry; D020027:Pyrococcus furiosus / Q000201:enzymology*; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D012502:Sapogenins / Q000378:metabolism*; D013696:Temperature; D001617:beta-Glucosidase / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*
D036145:Ginsenosides; D010936:Plant Extracts; D011994:Recombinant Proteins; D012502:Sapogenins; D001617:beta-Glucosidase; C112772:ginsenoside compound K; C062916:protopanaxadiol