Some biomolecules and a partially O-acetylated exo-galactomannan containing β-Galf units from pathogenic Exophiala jeanselmei, having a pronounced immunogenic response.
The pathogenic fungus Exophiala jeanselmei (Ej4) was grown in submerged MM medium, glucose being consumed after six days with maximum biomass and EPS production. Cells were extracted with CHCl3-MeOH (2:1, v/v) yielding a product containing 10% lipid, with high levels of unsaturated C(18:1) (43.6%) and C(18:2) (21.0%), 2D-TLC showed the presence of PE (17.7%), PS (11.6%), PC (35.8%), PI (1.2%) and lyso-phospholipids, LPE (10.7%), LPC (2.0%), PA (10.4%), cardiolipin (10.5%) and glucosyl-ceramide. Analysis of EPS-1 (120 kDa) showed a galactomanan, containing a main chain of Manp-(1→2) (24.2%), substituted by side chains containing terminal Galf (16.8%) and Manp (3.5%) and acetyl groups attached at O-6 of terminal Galf. An immune response against antigens was obtained using Balb/C mice. Anti-EPS-1 antibodies recognized purified fraction containing cellular walls very titer and higher than 1:20,000 for EPS. The studied biomolecules showed biotechnological potential and point to important perspectives in diagnosis of fungi and immunomodulatory products.
Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Curitiba-PR, Brazil. sassaki@ufpr.br
G L
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Sassaki
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Czelusniak
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Vicente
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Zanata
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Souza
P A J
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Gorin
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Iacomini
D000107:Acetylation; D000818:Animals; D018533:Biomass; D002850:Chromatography, Gel; D005093:Exophiala / Q000276:immunology* / Q000472:pathogenicity*; D005227:Fatty Acids / Q000032:analysis; D005690:Galactose / Q000737:chemistry / Q000276:immunology*; D006017:Glycolipids / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy; D008351:Mannans / Q000276:immunology*; D008745:Methylation; D051379:Mice; D008807:Mice, Inbred BALB C; D010743:Phospholipids / Q000032:analysis; D011134:Polysaccharides / Q000276:immunology / Q000302:isolation & purification
D005227:Fatty Acids; D006017:Glycolipids; D008351:Mannans; D010743:Phospholipids; D011134:Polysaccharides; D005690:Galactose