Transgenic barley with overexpressed PTrx increases aluminum resistance in roots during germination.
A transgenic barley line (LSY-11-1-1) with overexpressed Phalaris coerulescens thioredoxin gene (PTrx) was employed to measure the growth, protein oxidation, cell viability, and antioxidase activity in barley roots during germination on the presence of 2 mmol/L AlCl(3) on filter paper. The results show that (1) compared with the non-transgenic barley, LSY-11-1-1 had enhanced root growth, although both were seriously inhibited after AlCl(3) treatment; (2) the degree of protein oxidation and loss of cell viability in roots of LSY-11-1-1 were much less than those in roots of non-transgenic barley, as reflected by lower contents of protein carbonyl and Evans blue uptakes in LSY-11-1-1; (3) activities of catalase (CAT), glutathione peroxidase (GPX), ascorbate peroxidase (APX), and glutathione reductase (GR) in LSY-11-1-1 root tips were generally higher than those in non-transgenic barley root tips, although these antioxidase activities gave a rise to different degrees in both LSY-11-1-1 and non-transgenic barley under aluminum stress. These results indicate that overexpressing PTrx could efficiently protect barley roots from oxidative injury by increasing antioxidase activity, thereby quenching ROS caused by AlCl(3) during germination. These properties raise the possibility that transgenic barley with overexpressed PTrx may be used to reduce the aluminum toxicity in acid soils.
National Engineering Research Center for Wheat, Henan Agricultural University, Zhengzhou, China.
Qiao-yun
QY
Li
Hong-bin
HB
Niu
Jun
J
Yin
Hong-bo
HB
Shao
Ji-shan
JS
Niu
Jiang-ping
JP
Ren
Yong-chun
YC
Li
Xiang
X
Wang
D000535:Aluminum / Q000494:pharmacology*; D004351:Drug Resistance / Q000187:drug effects / Q000502:physiology; D024861:Genetic Enhancement / Q000379:methods; D018525:Germination / Q000187:drug effects / Q000502:physiology*; D001467:Hordeum / Q000187:drug effects / Q000502:physiology*; D031783:Phalaris / Q000235:genetics*; D018517:Plant Roots / Q000187:drug effects / Q000502:physiology*; D030821:Plants, Genetically Modified / Q000187:drug effects / Q000502:physiology*; D013879:Thioredoxins / Q000502:physiology*; D014162:Transfection
D013879:Thioredoxins; D000535:Aluminum