A simple set of plasmids for the production of transgenic plants.
A new set of plasmids for plant transgenic studies was developed. Its strong point is that independent gene cassettes are connected within one binary vector by the restriction endonuclease-based technique only. Using the set, two overexpressing cassettes and three RNA interference (RNAi) cassettes were successfully introduced into rice. Our plasmid set is useful for producing commercial transgenic plants, especially in the case of rice.
Rice Physiology Research Team, National Agricultural Research Center (NARC), Joetsu, Niigata, Japan. kurodama@affrc.go.jp
Masaharu
M
Kuroda
Mayumi
M
Kimizu
Chikako
C
Mikami
D001709:Biotechnology / Q000379:methods; D004262:DNA Restriction Enzymes; D005822:Genetic Vectors; D012275:Oryza / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000235:genetics*; D010957:Plasmids / Q000235:genetics*; D034741:RNA, Small Interfering
D034741:RNA, Small Interfering; D004262:DNA Restriction Enzymes