TP53 gene polymorphisms and breast cancer in Croatian women: a pilot study.
A case-control retrospective association study was conducted to investigate a possible association of the TP53 polymorphisms, Arg72Pro and PIN3 (+16bp), with sporadic breast cancer in Croatian women. Ninety-five women with breast cancer and 108 age-matched healthy women were analyzed. Arg72Pro polymorphism was detected by TaqMan essay. For designation of PIN3 (+16bp) polymorphism DNA amplification was performed by the polymerase chain reaction (PCR) while the PCR products were detected by capillary electrophoresis. Homozygous genotype of minor allele of the PIN3 (+16bp) polymorphism was associated with sporadic breast cancer (OR = 2.15, 95% confidence interval [CI] 1.80-2.56, p = 0.006). For Arg72 polymorphism, the odds ratio for breast cancer of ArgPro versus reference genotype ArgArg was 0.55 (95% CI 0.30-1.02, p = 0.039) suggesting the protective effect. Although different haplotypes did not influence the susceptibility to the disease, the joint occurrence of genotype combination ProPro/A2A2 frequent in cases, was associated with sporadic breast cancer (OR = 2.20, 95% CI 1.89-2.56, p = 0.021). The study provides evidence of the association of the TP53 gene polymorphisms Arg72Pro and PIN3 (+16bp) with sporadic breast cancer in the Croatian population.
Department of Oncology, Clinical Hospital Centre Zagreb, Croatia. vesna.bisof@zg.t-com.hr
V
V
Bisof
M Pericić
MP
Salihović
N Smolej
NS
Narancić
T
T
Skarić-Jurić
J
J
Jakić-Razumović
B
B
Janićijević
S
S
Turek
P
P
Rudan
D000328:Adult; D000368:Aged; D001943:Breast Neoplasms / Q000453:epidemiology / Q000235:genetics*; D017523:Croatia / Q000453:epidemiology; D005260:Female; D016158:Genes, p53; D006239:Haplotypes; D006801:Humans; D008875:Middle Aged; D016017:Odds Ratio; D010865:Pilot Projects; D020641:Polymorphism, Single Nucleotide; D012189:Retrospective Studies