COX-2 Gene increases tongue cancer cell proliferation and invasion through VEGF-C pathway.
COX-2 induces the proliferation and invasion of oral squamous cell carcinoma. In the present study, the role of the COX-2 gene in the tongue cancer cell proliferation and invasion was investigated. A short hairpin RNA (shRNA)method was used to knock down COX-2 gene expression and investigate the relationship between COX-2 and VEGF-C, and the role of the COX-2 gene for proliferation and invasion was also investigated in the tongue cancer cell Tca8113. COX-2 gene overexpressed in tongue cancer cell line. Suppressing the expression of COX-2 by shRNA could decrease cell proliferation comparing with control shRNA. Nevertheless, depressing COX-2 gene expression by shRNA reduced VEGF-C expression on both mRNA and protein levels. VEGF-C gene expression could be regulated by COX-2 gene. Our results suggested that COX-2 played essential roles in the proliferation and metastasis of tongue cancer, and COX-2 could serve as a potential chemotherapy target for tongue cancer.
Department of Head and Neck Surgery, Sun Yat-sen University Cancer Center, No 651 Dongfeng East Road, Guangzhou, Guangdong, People's Republic of China.
Yan-Hong
YH
Wang
Ming-Wei
MW
Wu
An-Kui
AK
Yang
Wei-Dong
WD
Zhang
Jian
J
Sun
Tian-Run
TR
Liu
Yan-Feng
YF
Chen
D045744:Cell Line, Tumor; D049109:Cell Proliferation; D051546:Cyclooxygenase 2 / Q000378:metabolism*; D006801:Humans; D009361:Neoplasm Invasiveness; D015398:Signal Transduction; D014062:Tongue Neoplasms / Q000378:metabolism* / Q000473:pathology; D042582:Vascular Endothelial Growth Factor C / Q000378:metabolism*
C467486:VEGFC protein, human; D042582:Vascular Endothelial Growth Factor C; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human