Diurnal variation and effect of insulin on circulating high molecular weight (HMW) adiponectin and NF-κB activity in human endothelial cells.
OBJECTIVE
To study the diurnal variation and the effect of insulin on adiponectin multimers and nuclear factor-kappaB (NF-κB) activity in human endothelial cells.


RESULTS
We utilized a prolonged insulin-glucose infusion in six healthy human subjects. HMW and total adiponectin levels were higher in the morning and lower at night; NF-κB activities in serum treated human microvascular endothelial cells (HMEC-1) cells were lower in the morning and higher at night. Hyperinsulinemic induction significantly decreased HMW and total adiponectin levels but increased NF-κB activity in serum treated HMEC-1 cells (P<0.05, P<0.01). There were no significant changes to MMW and LMW adiponectin levels (P>0.05).


CONCLUSIONS
Circadian rhythm of HMW adiponectin and NF-κB activity are altered by hyperinsulinemia providing novel insights adiponectin and NF-κB biology, which may be pertinent to insulin resistant states, e.g. obesity and type 2 diabetes mellitus.
Clinical Sciences Research Institute, Warwick Medical School, University of Warwick, Coventry CV4 7AL, UK. B.K.Tan.1@warwick.ac.uk
Bee K
BK
Tan
Raghu
R
Adya
Krzysztof C
KC
Lewandowski
J Paul
JP
O'Hare
Harpal S
HS
Randeva
D052242:Adiponectin / Q000097:blood*; D000328:Adult; D001786:Blood Glucose / Q000378:metabolism; D002940:Circadian Rhythm; D003924:Diabetes Mellitus, Type 2 / Q000097:blood; D042783:Endothelial Cells / Q000378:metabolism*; D005260:Female; D006801:Humans; D006946:Hyperinsulinism / Q000378:metabolism; D007328:Insulin / Q000097:blood*; D007333:Insulin Resistance; D008297:Male; D008833:Microcirculation; D008954:Models, Biological; D008970:Molecular Weight; D016328:NF-kappa B / Q000378:metabolism*; D009765:Obesity / Q000097:blood
D052242:Adiponectin; D001786:Blood Glucose; D007328:Insulin; D016328:NF-kappa B