Gold nanoparticles inhibited the receptor activator of nuclear factor-κb ligand (RANKL)-induced osteoclast formation by acting as an antioxidant.
Gold nanoparticles inhibited osteoclast (OC) formation induced by the receptor activator of nuclear factor-κB ligand (RANKL) in bone marrow-derived macrophages (BMMs). This was accompanied by a decreased level of tartrate-resistant alkaline phosphatase (TRAP) and less activation of nuclear factor (NF)-κB. The nanoparticles also reduced the production of reactive oxygen species (ROS) in response to RANKL and upregulated RANKL-induced glutathione peroxidase-1 (Gpx-1), suggesting a role as an antioxidant in the BMM. The inhibitory effects on OC formation might have been due to elevated defense against oxidative stress.
Department of Biological Sciences (BK21 Program) and Immunomodulation Research Center, University of Ulsan, Korea.
Ok-Joo
OJ
Sul
Jin-Chun
JC
Kim
Tae-Wook
TW
Kyung
Hye-Jin
HJ
Kim
Youn-Young
YY
Kim
Song-Hee
SH
Kim
Ji-Soon
JS
Kim
Hye-Seon
HS
Choi
D000818:Animals; D000975:Antioxidants; D005979:Glutathione Peroxidase / Q000235:genetics*; D006046:Gold; D008264:Macrophages; D053768:Metal Nanoparticles / Q000737:chemistry*; D051379:Mice; D008810:Mice, Inbred C57BL; D010010:Osteoclasts / Q000166:cytology*; D053245:RANK Ligand / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism; D053246:Receptor Activator of Nuclear Factor-kappa B / Q000037:antagonists & inhibitors*; D015854:Up-Regulation / Q000235:genetics; D000094765:Glutathione Peroxidase GPX1
D000975:Antioxidants; D053245:RANK Ligand; D017382:Reactive Oxygen Species; D053246:Receptor Activator of Nuclear Factor-kappa B; D006046:Gold; D005979:Glutathione Peroxidase; D000094765:Glutathione Peroxidase GPX1; C000722068:Gpx1 protein, mouse