External irradiation models for intracranial 9L glioma studies.
OBJECTIVE
Radiotherapy has been shown to be an effective for the treatment human glioma and consists of 30 fractions of 2 Gy each for 6-7 weeks in the tumor volume with margins. However. in preclinical studies, many different radiation schedules are used. The main purpose of this work was to review the relevant literature and to propose an external whole-brain irradiation (WBI) protocol for a rat 9L glioma model.


METHODS
9L cells were implanted in the striatum of twenty 344-Fisher rats to induce a brain tumor. On day 8, animals were randomized in two groups: an untreated group and an irradiated group with three fractions of 6 Gy at day 8, 11 and 14. Survival and toxicity were assessed.


RESULTS
Irradiated rats had significantly a longer survival (p = 0.01). No deaths occurred due to the treatment. Toxicities of reduced weight and alopecia were increased during the radiation period but no serious morbidity or mortality was observed. Moreover, abnormalities disappeared the week following the end of the therapeutic schedule.


CONCLUSIONS
Delivering 18 Gy in 3 fractions of 6 Gy every 3 days, with mild anaesthesia, is safe, easy to reproduce and allows for standardisation in preclinical studies of different treatment regimens glioma rat model.
INSERM, U646, Université d'Angers, Angers, F-49100, France. skaya2@free.fr
Sandrine
S
Vinchon-Petit
Delphine
D
Jarnet
Eric
E
Jadaud
Loïc
L
Feuvret
Emmanuel
E
Garcion
Philippe
P
Menei
D000818:Animals; D001932:Brain Neoplasms / Q000532:radiotherapy*; D016371:Cranial Irradiation / Q000379:methods*; D004195:Disease Models, Animal; D019583:Dose Fractionation, Radiation; D005260:Female; D005910:Glioma / Q000532:radiotherapy*; D051381:Rats; D011916:Rats, Inbred F344