[Human papillomavirus genotyping in cervical adenocarcinoma in the Region of La Araucania-Chile].
Human papillomavirus (HPV) is the main cause of cervical cancer. Thus, HPV detection and typing becomes important in order to know the frequency of genotypes present in the region. In this paper we studied 44 biopsies of cervical adenocarcinoma. For HPV detection nested polymerase chain reaction (PCR) was used to amplify the L1 gene. For viral typing restriction enzymes (Rsa I, Dde I, Pst I) and DNA sequencing were used. Viral DNA was detected by nested L1 PCR in 100% of biopsies; 38/44 cases could be typed: 81.6% HPV16; 13.2% HPV 18; 2.6% VPH 33 and 2.6% HPV 18/33.


CONCLUSIONS
The technique was successful in identifying the virus type in 86% of biopsies. There was a strong association ACC-HPV, especially with the viral type 16, detected in 81.6% of established cases.
Universidad de La Frontera, Facultad de Medicina, Departamento de Anatomía Patológica.
Angélica
A
Melo
Patricia
P
García
Italo
I
Capurro
Pablo
P
Guzmán
Priscila
P
Brebi
Carmen
C
Ili
Jaime
J
López
Juan C
JC
Roa
D000230:Adenocarcinoma / Q000821:virology*; D000328:Adult; D000368:Aged; D052685:Alphapapillomavirus / Q000235:genetics*; D002677:Chile; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D030361:Papillomavirus Infections / Q000821:virology*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012189:Retrospective Studies; D002583:Uterine Cervical Neoplasms / Q000821:virology*
D004279:DNA, Viral