Loop-mediated isothermal amplification assay targeting the ompA gene for rapid detection of Riemerella anatipestifer.
A novel loop-mediated isothermal amplification (LAMP) assay was developed and evaluated for the detection of Riemerella anatipestifer (RA) infection. The LAMP assay exhibited a higher sensitivity than conventional polymerase chain reaction (PCR) and microbial isolation. The specificity of the assay was determined by restriction enzyme digestion of the LAMP products and detection of Escherichia coli, Salmonella enterica and Pasteurella multocida. The LAMP assay was able to detect RA effectively in samples of the reference strains, isolated strains and infected duck brains. This assay is a useful tool for the diagnosis of RA infection in the clinical setting.
State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 1 Xujiaping, Yanchangbao, Lanzhou 730046, China.
Fuying
F
Zheng
Guozhen
G
Lin
Jizhang
J
Zhou
Guanghua
G
Wang
Xiaoan
X
Cao
Xiaowei
X
Gong
Changqing
C
Qiu
D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D001921:Brain / Q000821:virology; D004372:Ducks / Q000821:virology; D045826:Flavobacteriaceae Infections / Q000175:diagnosis / Q000662:veterinary; D005417:Flavobacterium / Q000235:genetics*; D057230:Limit of Detection; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*
D001425:Bacterial Outer Membrane Proteins; C078167:OMPA outer membrane proteins