[Transfection of wild-type p14ARF gene leads to growth inhibition of human lung cancer cell lines].
BACKGROUND
To examine whether wild-type p14ARF gene is a candidate suppressor gene for lung cancer.


METHODS
Human lung cancer cell lines having various endogenous backgrounds in INK4a, p53 and Rb genes were used as the recipients of the wild-type p14ARF gene. The expression of p14ARF mRNA and protein was detected with RT-PCR, immunohistochemistry and Western blot after G418 selection. Clones which expressed both p14ARF mRNA and protein were identified and selected for further experiements. By comparing with the parental and negative control cells treated with empty vectors, the effects of exogenously transfected p14ARF on cell division rate, cell cycle distribution and morphologic alteration were analyzed. In vivo evaluation of the growth rate was also made with the experiment of nude mice tumor formation.


RESULTS
Upon transfection with p14ARF gene, cells were arrested at G1 or G1/G2 phase of cell cycle in three wtp53 lung cancer cell lines and their proliferation rates were also inhibited.


CONCLUSIONS
Human wild-type p14ARF gene has suppressive effect on abnormal proliferation of lung cancer cells, especially in some wtp53 lung cancer cells, and it might be an ideal candidate for gene therapy of human lung cancer.
Cancer Institute, Medical Center of Fudan University, Shanghai 200032, P.R.China.
N
N
Gao
Y
Y
Hu
K
K
Huang
X
X
Cao
J
J
Zhou
S
S
Cao