Small GTPases of the Rab family in the brain of Bombyx mori.
Small GTPases of the Rab family are key regulators of membrane trafficking. We produced antibodies against the Rab7 protein of Bombyx mori (BRab7) in rabbits, and against the Rab11 protein of B. mori (BRab11) in mice. The antibodies recognized BRab7 and BRab11 proteins, but did not recognize other Rab proteins. Immunoblotting of samples from brain tissue of B. mori revealed a single band for each antibody. Rab11 was expressed in most tissues, whereas Rab7 was expressed in the brain, ovary, and testis. Immunohistochemical reactivity of Rab7 and Rab11 in the brain of B. mori was restricted to neurons of the pars intercerebralis and dorsolateral protocerebrum. Double-labeling experiments demonstrated that immunohistochemical reactivity of Rab7 co-localized with that of Rab11 and partially with that of Rab8. Immunohistochemical reactivity of Rab11 and Rab8 co-localized with that of PERIOD, one of the proteins associated with circadian rhythm. These findings suggest that Rab7, Rab8, and Rab11 are involved in protein transport in the neurons of the brain of B. mori and might play a role in the control of circadian rhythm.
Department of Biofunctional Chemistry, Faculty of Agriculture, Kobe University, Nada-ku, Kobe, Hyogo, Japan. unotom@kobe-u.ac.jp
Tomohide
T
Uno
Keisuke
K
Hata
Susumu
S
Hiragaki
Yuri
Y
Isoyama
Le Thi Dieu
le TD
Trang
Yuichi
Y
Uno
Kengo
K
Kanamaru
Hiroshi
H
Yamagata
Masahiko
M
Nakamura
Michihiro
M
Takagi
Makio
M
Takeda
D000818:Animals; D012831:Bombyx; D001921:Brain / Q000201:enzymology*; D002940:Circadian Rhythm / Q000502:physiology; D007150:Immunohistochemistry; D051379:Mice; D056950:Period Circadian Proteins / Q000378:metabolism; D011817:Rabbits; D020691:rab GTP-Binding Proteins / Q000276:immunology / Q000378:metabolism*; D000090802:rab7 GTP-Binding Proteins; D000099193:rab11 GTP-Binding Proteins
D056950:Period Circadian Proteins; D020691:rab GTP-Binding Proteins; D000090802:rab7 GTP-Binding Proteins; D000099193:rab11 GTP-Binding Proteins; C000716465:rab7 GTP-binding proteins, mouse