Ozone induces inflammation in bronchial epithelial cells.
OBJECTIVE
Ozone is a main component of secondary pollutants of vehicle exhausts, and ozone exposure to children in urban areas may be associated with the development of asthma. However, little is known about the mechanism(s) by which ozone affects human airway epithelium and subsequent airway inflammation.


METHODS
Human bronchial epithelial cells were exposed to ozone at 0.16 mg/m(3) for varying periods. The concentrations of IL-1 and IL-6 secreted by the cells were measured by enzyme-linked immunosorbent assay (ELISA) and the contents of intracellular malondialdehyde (MDA) were determined. Furthermore, the conditional medium from the ozone-exposed cells was examined for stimulating human peripheral T lymphocytes from asthmatic patients and healthy subjects, and the production of cytokines was characterized by ELISA and quantitative real-time polymerase chain reaction (RT-PCR).


RESULTS
Ozone stimulated the IL-1 and IL-6 production by BEAS-2B cells and its stimulatory effects were time dependent. Furthermore, ozone exposure significantly increased the levels of MDA in BEAS-2B cells, as compared with that of the cells without ozone exposure, in a time-dependent manner. In addition, the conditional medium from the cells exposed to ozone, but not control condition medium, significantly increased the relative levels of IL-1 mRNA transcripts in human peripheral T lymphocytes from asthmatic patients, but not healthy subjects. However, the conditional medium did not induce significantly increased levels of IL-2 production by peripheral T cells.


CONCLUSIONS
Our data indicated that exposure to low levels of ozone for a short period induced increases in the pro-inflammatory markers and oxidative stress in epithelial cells, which might contribute to airway inflammation particularly in asthmatic children.
Department of Preventive Medicine, School of Public Health, Sun Yat-Sen University, Guangzhou, P.R. China. songhong@mail.sysu.edu.cn
Hong
H
Song
Weipin
W
Tan
Xuchao
X
Zhang
D001249:Asthma / Q000378:metabolism; D001980:Bronchi / Q000187:drug effects* / Q000378:metabolism; D002460:Cell Line; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007249:Inflammation; D007375:Interleukin-1 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D010126:Ozone / Q000009:adverse effects*; D013601:T-Lymphocytes / Q000378:metabolism
D007375:Interleukin-1; D015850:Interleukin-6; D010126:Ozone