Activation of NF-κB by nucleocapsid protein of the porcine reproductive and respiratory syndrome virus.
Nuclear factor kappa B (NF-κB) is a critical transcription factor in innate and adaptive immune response as well as cell proliferation and survival. Previous studies have demonstrated that porcine reproductive and respiratory syndrome virus (PRRSV) infection activated NF-κB pathways through IκB degradation in MARC-145 cells and alveolar macrophages. To evaluate the mechanisms behind this, we investigated the role of PRRSV structural proteins in the regulation of NF-κB. In this study, we screened the structural proteins of PRRSV by NF-κB DNA-binding assay and luciferase activity assay and demonstrated that PRRSV nucleocapsid (N) protein could activate NF-κB in MARC-145 cells. Furthermore, we revealed that the region between aa 30 and 73 of N protein was essential for its function in the activation of NF-κB. These results presented here provide a basis for understanding molecular mechanism of PRRSV infection and inflammation response.
State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China.
Rui
R
Luo
Liurong
L
Fang
Yunbo
Y
Jiang
Hui
H
Jin
Yanwei
Y
Wang
Dang
D
Wang
Huanchun
H
Chen
Shaobo
S
Xiao
D000818:Animals; D002460:Cell Line; D005786:Gene Expression Regulation; D016328:NF-kappa B / Q000378:metabolism*; D019590:Nucleocapsid Proteins / Q000235:genetics / Q000378:metabolism*; D019316:Porcine respiratory and reproductive syndrome virus / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity*; D013552:Swine
D016328:NF-kappa B; D019590:Nucleocapsid Proteins