Expression and RNA interference of salivary polygalacturonase genes in the tarnished plant bug, Lygus lineolaris.
Three genes encoding polygalacturonase (PG) have been identified in Lygus lineolaris (Palisot de Beauvois) (Miridae: Hemiptera). Earlier studies showed that the three PG gene transcripts are exclusively expressed in the feeding stages of L. lineolaris. In this report, it is shown that all three transcripts are specifically expressed in salivary glands indicating that PGs are salivary enzymes. Transcriptional profiles of the three PGs were evaluated with respect to diet, comparing live cotton plant material to artificial diet. PG2 transcript levels were consistently lower in cotton-fed insects than those reared on artificial diet. RNA interference was used to knock down expression of PG1 mRNA in adult salivary glands providing the first demonstration of the use of this method in the non-model insect, L. lineolaris.
USDA-ARS, Biological Control of Pests Research Unit, 59 Lee Road, NBCL, Building 8, Stoneville, Mississippi, USA. william.walker@ARS.USDA.GOV
William B
WB
Walker
Margaret L
ML
Allen
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004032:Diet; D020869:Gene Expression Profiling; D003368:Gossypium; D020062:Heteroptera / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D011096:Polygalacturonase / Q000235:genetics* / Q000378:metabolism; D016133:Polymerase Chain Reaction; D034622:RNA Interference; D012469:Salivary Glands / Q000201:enzymology*; D017422:Sequence Analysis, DNA
D011096:Polygalacturonase