Cancer detection by native fluorescence of urine.
Because cancer is a dreaded disease, a number of techniques such as biomarker evaluation, mammograms, colposcopy, and computed tomography scan are currently employed for early diagnosis. Many of these are specific to a particular site, invasive, and often expensive. Hence, there is a definite need for a simple, generic, noninvasive protocol for cancer detection, comparable to blood and urine tests for diabetes. Our objective is to show the results of a novel study in the diagnosis of several cancer types from the native or intrinsic fluorescence of urine. We use fluorescence emission spectra (FES) and stokes shift spectra (SSS) to analyze the native fluorescence of the first voided urine samples of healthy controls (N=100) and those of cancer patients (N=50) of different etiology. We show that flavoproteins and porphyrins released into urine can act as generic biomarkers of cancer with a specificity of 92%, a sensitivity of 76%, and an overall accuracy of 86.7%. We employ FES and SSS for rapid and cost-effective quantification of certain intrinsic biomarkers in urine for screening and diagnosis of most common cancer types with an overall accuracy of 86.7%.
King Saud University, College of Science, Department of Physics, P.O. Box 2455, Riyadh, Saudi Arabia 11451. masila123@gmail.com
Vadivel
V
Masilamani
Trinka
T
Vijmasi
Mohammad
M
Al Salhi
Kanagaraj
K
Govindaraj
Ayanam Parthasarathy
AP
Vijaya-Raghavan
Belavendra
B
Antonisamy
D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000652:urine*; D016022:Case-Control Studies; D016002:Discriminant Analysis; D005260:Female; D005420:Flavoproteins / Q000652:urine; D005453:Fluorescence; D006801:Humans; D008297:Male; D008875:Middle Aged; D009369:Neoplasms / Q000175:diagnosis* / Q000652:urine*; D055620:Optical Phenomena; D011166:Porphyrins / Q000652:urine; D011237:Predictive Value of Tests; D012372:ROC Curve; D013050:Spectrometry, Fluorescence / Q000379:methods*; D016482:Urinalysis / Q000379:methods; D014556:Urine / Q000737:chemistry*
D014408:Biomarkers, Tumor; D005420:Flavoproteins; D011166:Porphyrins