Hemoglobin degradation in human erythrocytes with long-duration near-infrared laser exposure in Raman optical tweezers.
Near-infrared laser (785-nm)-excited Raman spectra from a red blood cell, optically trapped using the same laser beam, show significant changes as a function of trapping duration even at trapping power level of a few milliwatts. These changes in the Raman spectra and the bright-field images of the trapped cell, which show a gradual accumulation of the cell mass at the trap focus, suggest photoinduced aggregation of intracellular heme. The possible role of photoinduced protein denaturation and hemichrome formation in the observed aggregation of heme is discussed.
Raja Ramanna Centre for Advanced Technology, Laser Biomedical Applications and Instrumentation Division, Indore 452013, India. raktim@rrcat.gov.in
Raktim
R
Dasgupta
Sunita
S
Ahlawat
Ravi Shanker
RS
Verma
Abha
A
Uppal
Pradeep Kumar
PK
Gupta
D004912:Erythrocytes / Q000378:metabolism* / Q000528:radiation effects*; D006454:Hemoglobins / Q000737:chemistry / Q000378:metabolism* / Q000528:radiation effects*; D006801:Humans; D066298:In Vitro Techniques; D007259:Infrared Rays / Q000009:adverse effects*; D053844:Lasers, Solid-State / Q000009:adverse effects; D052898:Optical Tweezers; D055668:Photochemical Processes; D011489:Protein Denaturation / Q000528:radiation effects; D013059:Spectrum Analysis, Raman / Q000295:instrumentation
D006454:Hemoglobins