EM-ISEL: a useful tool to visualize DNA damage at the ultrastructural level.
A method for the localization of DNA strand breaks at the ultrastructural level is presented. The technique involves the use of terminal deoxynucleotidyl transferase and labeled dUTP. Incorporation of labeled nucleotides is visualized through colloidal gold labeling. Cells undergoing apoptotic or necrotic cell death, as well as cells showing death-unrelated DNA damage, can be easily distinguished. The technique uses tissues routinely processed for electron microscopy. It has been successfully applied to study DNA damage and apoptosis in different pathologic conditions. The feasibility of this technique for retrospective studies on archival material is emphasized.
Centro Regionale Diagnosi ed Osservazione delle Malattie Prioniche DOMP-ASL TO2, Turin, Italy. a.migheli@gmail.com
Antonio
A
Migheli
D000818:Animals; D004247:DNA / Q000648:ultrastructure*; D004249:DNA Damage; D020287:In Situ Nick-End Labeling / Q000379:methods*; D008854:Microscopy, Electron / Q000379:methods*
D004247:DNA