Efficient regeneration by activation of neurogenesis in homeostatically quiescent regions of the adult vertebrate brain.
In contrast to mammals, salamanders and teleost fishes can efficiently repair the adult brain. It has been hypothesised that constitutively active neurogenic niches are a prerequisite for extensive neuronal regeneration capacity. Here, we show that the highly regenerative salamander, the red spotted newt, displays an unexpectedly similar distribution of active germinal niches with mammals under normal physiological conditions. Proliferation zones in the adult newt brain are restricted to the forebrain, whereas all other regions are essentially quiescent. However, ablation of midbrain dopamine neurons in newts induced ependymoglia cells in the normally quiescent midbrain to proliferate and to undertake full dopamine neuron regeneration. Using oligonucleotide microarrays, we have catalogued a set of differentially expressed genes in these activated ependymoglia cells. This strategy identified hedgehog signalling as a key component of adult dopamine neuron regeneration. These data show that brain regeneration can occur by activation of neurogenesis in quiescent brain regions.
Karolinska Institute, Department for Cell and Molecular Biology, Stockholm 17177, Sweden.
Daniel A
DA
Berg
Matthew
M
Kirkham
Anna
A
Beljajeva
Dunja
D
Knapp
Bianca
B
Habermann
Jesper
J
Ryge
Elly M
EM
Tanaka
András
A
Simon
D000818:Animals; D001921:Brain / Q000166:cytology* / Q000378:metabolism*; D004298:Dopamine / Q000378:metabolism; D018274:Electroporation; D007150:Immunohistochemistry; D008636:Mesencephalon / Q000166:cytology / Q000378:metabolism; D055495:Neurogenesis / Q000235:genetics / Q000502:physiology*; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D016627:Oxidopamine / Q000378:metabolism; D016548:Prosencephalon / Q000166:cytology / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014562:Caudata / Q000378:metabolism; D014714:Vertebrates / Q000378:metabolism*
D016627:Oxidopamine; D004298:Dopamine