[Increase of immunogenicity of cold-adapted influenza vaccine by using adjuvant].
OBJECTIVE
To assess increase of protective efficacy of live cold-adapted (ca) influenza vaccine after addition of adjuvant chitozan.


METHODS
Used viruses: ca donor of attenuation A/Krasnodar/101/35/59 (H2N2) and epidemic strain A/Krasnodar/101/59 (H2N2); as an adjuvant--derivative of chitozan and microparticles of chitozan. Experiments were performed in outbred mice. Protective effect of immunization was measured by intranasal challenge by virulent strain of virus. Immune response was assessed by ELISA and indirect hemagglutination inhibition assay.


RESULTS
During intranasal immunization of mice with intact CA donor of attenuation A/Krasnodar/101/35/59 (H2N2) addition of 1% solution of chitozan glutamate to vaccine material resulted in increased serum IgG in immunized mice and protective effect of immunization. Addition of adjuvant to ca donor strain did not influence on its ts-characteristic. It was shown that inactivated with ultraviolet radiation ca donor strain in combination with chitozan did not protect against infection caused by virulent strain A/Krasnodar/101/59, whereas the same doses of intact ca donor strain with chitozan were protective. Chitozan did not enhance replication of donor strain in upper respiratory tract of mice.


CONCLUSIONS
Obtained data demonstrate that chitozan as a mucous-adhesive adjuvant could increase efficacy of live ca influenza vaccine.
S G
SG
Markushin
Iu Z
IuZ
Gendon
G G
GG
Krivtsov
I I
II
Akopova
A S
AS
Sukhno
A D
AD
Pereverzev
D000276:Adjuvants, Immunologic; D000281:Administration, Intranasal; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D048271:Chitosan / Q000008:administration & dosage / Q000276:immunology*; D004353:Drug Evaluation, Preclinical; D053121:Influenza A Virus, H2N2 Subtype / Q000276:immunology*; D007252:Influenza Vaccines / Q000008:administration & dosage / Q000276:immunology* / Q000528:radiation effects; D051379:Mice; D009976:Orthomyxoviridae Infections / Q000097:blood / Q000517:prevention & control*; D014613:Vaccines, Attenuated / Q000008:administration & dosage / Q000276:immunology / Q000528:radiation effects
D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D007252:Influenza Vaccines; D014613:Vaccines, Attenuated; D048271:Chitosan