Interaction of metal oxide nanoparticles with lung surfactant protein A.
The alveolar lining fluid (ALF) covering the respiratory epithelium of the deep lung is the first biological barrier encountered by nanoparticles after inhalation. We here report for the first time significant differences for metal oxide nanoparticles to the binding of surfactant protein A (SP-A), the predominant protein component of ALF. SP-A is a physiologically most relevant protein and provides important biological signals. Also, it is involved in the lung's immune defence, controlling e.g. particle binding, uptake or transcytosis by epithelial cells and macrophages. In our study, we could prove different particle-protein interaction for eight different nanoparticles, whereas particles of the same bulk material revealed different adsorption patterns. In contrast to other proteins as bovine serum albumin (BSA), SP-A does not seem to significantly deagglomerate large agglomerates of particles, indicating different adsorption mechanisms as in the well-investigated model protein BSA. These findings may have important consequences for biological fate and toxicological effects of inhaled nanomaterials.
Department of Biopharmaceutics and Pharmaceutical Technology, Saarland University, Saarbruecken, Germany. chr.schulze@mx.uni-saarland.de
Christine
C
Schulze
Ulrich F
UF
Schaefer
Christian A
CA
Ruge
Wendel
W
Wohlleben
Claus-Michael
CM
Lehr
D000327:Adsorption; D000818:Animals; D015153:Blotting, Western; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D008168:Lung / Q000737:chemistry* / Q000378:metabolism; D008670:Metals / Q000008:administration & dosage / Q000737:chemistry* / Q000633:toxicity; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry* / Q000633:toxicity; D010087:Oxides / Q000008:administration & dosage / Q000737:chemistry* / Q000633:toxicity; D010316:Particle Size; D011485:Protein Binding; D037662:Pulmonary Surfactant-Associated Protein A / Q000737:chemistry*; D013499:Surface Properties; D013552:Swine; D014461:Ultracentrifugation
D008670:Metals; D010087:Oxides; D037662:Pulmonary Surfactant-Associated Protein A