Anti-inflammatory and enzyme inhibitory activities of a crude extract and a pterocarpan isolated from the aerial parts of Vitex agnus-castus.
A new compound, 6a,11a-dihydro-6H-[1] benzofuro [3,2-c][1,3]dioxolo[4,5-g]chromen-9-ol was isolated from the ethyl acetate fraction of Vitex agnus-castus. The structure of this compound was identified with the help of spectroscopic techniques ((13)C NMR, (1)H NMR, HMBC, HMQC, NOESY and COSY). The compound showed low urease- (32.0%) and chymotrypsin- (31.4%) inhibitory activity, and moderate (41.3%) anti-inflammatory activity. The crude extract and various fractions obtained from the aerial parts of the plant were also screened for possible in vitro hemagglutination, antibacterial and phytotoxic activities. No hemagglutination activity against human erythrocytes was observed in crude extracts and fractions of V. agnus-castus. The fractions and crude methanolic extract showed moderate and low antibacterial activity. Exceptions were the CHCl(3) fraction, which showed significant antibacterial activity against Klebsiella pneumonia (81% with MIC(50)=2.19 mg/mL), the n-hexane fraction, which exhibited no activity against Salmonella typhi, and the CHCl(3) and aqueous fractions, which showed no activity against Bacillus pumalis. Moderate phytotoxic activity (62.5%) was observed by n-hexane fraction of V. agnus-castus against Lemna minor L at 1000 μg/mL.
Center for Biotechnology and Microbiology University of Peshawar, Peshawar, Pakistan. bashirdr2001@yahoo.com
Bashir
B
Ahmad
Sadiq
S
Azam
Shumaila
S
Bashir
Ibrar
I
Khan
Achyut
A
Adhikari
Muhammad Iqbal
MI
Choudhary
D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000494:pharmacology; D001407:Bacillus / Q000187:drug effects; D002918:Chymotrypsin / Q000378:metabolism; D045424:Complex Mixtures / Q000737:chemistry / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D006384:Hemagglutination / Q000187:drug effects; D006801:Humans; D035261:Plant Components, Aerial / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D036343:Pterocarpans / Q000302:isolation & purification / Q000494:pharmacology*; D012485:Salmonella typhi / Q000187:drug effects; D014510:Urease / Q000378:metabolism; D020950:Vitex / Q000737:chemistry*
D000900:Anti-Bacterial Agents; D000893:Anti-Inflammatory Agents; D045424:Complex Mixtures; D004791:Enzyme Inhibitors; D010936:Plant Extracts; D036343:Pterocarpans; D002918:Chymotrypsin; D014510:Urease