[Specific accumulation and antitumor effects of hybrid liposomes on the growth of lung tumor cells].
In general, chemotherapeutic effects were low for non-small cell lung cancer (NSCLC) in the lung tumor. We examined the accumulation and antitumor effects of hybrid liposomes (HL-23) composed of phospholipid (L-α-dimyristoylphosphatidylcholine: DMPC) and PEG surfactant [polyoxyethylene(23)dodecyl ether: C₁₂(EO)₂₃] on NSCLC cells in vitro. Accumulation of HL-23 including a fluorescence probe [1-Palmitoyl-2-[12(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]dodecanoyl]-sn-Glycero-3-Phosphocholine: NBDPC] was observed for NSCLC cells using a confocal laser microscope, but no accumulation of HL-23 in normal lung cells was observed. Furthermore, inhibitory effects of HL-23 on the growth of NSCLC cells were obtained on the basis of a WST-1 assay. It was also clarified that HL-23 induced apoptosis for NSCLC cells on the basis of Annexin-V binding and TUNEL assay. These results suggest that HL-23 could be applied in effective chemotherapies for NSCLC.
Division of Applied Life Science, Graduate School of Engineering, Sojo University, Kumamoto, Japan.
Mamiko
M
Yukihara
Yuji
Y
Komizu
Osamu
O
Tanoue
Taku
T
Matsushita
Yoko
Y
Matsumoto
Ryuichi
R
Ueoka
D017209:Apoptosis / Q000187:drug effects; D002289:Carcinoma, Non-Small-Cell Lung / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology*; D002455:Cell Division / Q000187:drug effects; D004134:Dimyristoylphosphatidylcholine / Q000378:metabolism / Q000494:pharmacology* / Q000627:therapeutic use; D006801:Humans; D008081:Liposomes / Q000378:metabolism / Q000494:pharmacology* / Q000627:therapeutic use; D008175:Lung Neoplasms / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology*; D008560:Membrane Fluidity / Q000187:drug effects; D011092:Polyethylene Glycols / Q000378:metabolism / Q000494:pharmacology* / Q000627:therapeutic use; D014407:Tumor Cells, Cultured
D008081:Liposomes; D011092:Polyethylene Glycols; D004134:Dimyristoylphosphatidylcholine