Isolation of synthetic lethal mutations in the rsm1-null mutant of fission yeast.
To identify mutations in genes that are genetically linked to rsm1, we performed a synthetic lethal genetic screen in the fission yeast, Schizosaccharomyces pombe. Four mutations that showed synthetic lethality in combination with the rsm1null allele were isolated from approximately 320,000 colonies and defined in three complementation groups. One mutant (SLrsm1) exhibited a significant accumulation of poly(A)(+) RNA in the nucleus under synthetic lethal conditions, while the rest had no mRNA export defects. In addition, some genes (spmex67, rae1, or mlo3) required for mRNA export complemented the growth defects of the identified mutants. These results suggest that the isolated mutants contain mutations in genes that are involved in mRNA export and/or pre-mRNA retention.
School of Biological Sciences and Chemistry, Sungshin Women's University, Seoul 136-742, Republic of Korea.
DongGeRaMi
D
Moon
Yun-Sun
YS
Park
Cha-Yeon
CY
Kim
Jin Ho
JH
Yoon
D021581:Active Transport, Cell Nucleus; D020043:Genes, Essential; D005800:Genes, Fungal; D005816:Genetic Complementation Test; D050296:Microbial Viability; D009154:Mutation; D012333:RNA, Messenger / Q000378:metabolism; D012568:Schizosaccharomyces / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism / Q000502:physiology
D012333:RNA, Messenger