Performance evaluation of long monolithic silica capillary columns in gradient liquid chromatography using peptide mixtures.
A systematic study is reported on the performance of long monolithic capillary columns in gradient mode. Using a commercial nano-LC system, reversed-phase peptide separations obtained through UV-detection were conducted. The chromatographic performance, in terms of conditional peak capacity and peak productivity, was investigated for different gradient times (varying between 90 and 1320min) and different column lengths (0.25, 1, 2 and 4m) all originating from a single 4m long column. Peak capacities reaching values up to n=10(3) were measured in case of the 4m long column demonstrating the high potential of these long monoliths for the analysis of complex biological mixtures, amongst others. In addition, it was found that the different column fragments displayed similar flow resistance as well as consistent chromatographic performance in accordance with chromatographic theory indicating that the chromatographic bed of the original 4m long column possessed a structural homogeneity over its entire length.
Vrije Universiteit Brussel, Department of Chemical Engineering, Pleinlaan 2, B-1050 Brussels, Belgium. heghbali@vub.ac.be
Hamed
H
Eghbali
Koen
K
Sandra
Frederik
F
Detobel
Frederic
F
Lynen
Kazuki
K
Nakanishi
Pat
P
Sandra
Gert
G
Desmet
D056148:Chromatography, Reverse-Phase / Q000295:instrumentation* / Q000379:methods*; D004867:Equipment Design; D016014:Linear Models; D008855:Microscopy, Electron, Scanning; D010446:Peptide Fragments / Q000032:analysis / Q000302:isolation & purification; D011312:Pressure; D012822:Silicon Dioxide / Q000737:chemistry*
D010446:Peptide Fragments; D012822:Silicon Dioxide