Alternation of Venerupis philippinarum Hsp40 gene expression in response to pathogen challenge and heavy metal exposure.
HSP40 was an understudied protein family with co-chaperone activity. In the present study, a HSP40 homology was cloned from Venerupis philippinarum haemocytes (designated as VpHSP40) by EST analysis and RACE approaches. The expression profiles of VpHSP40 under Vibrio anguillarum challenge and heavy metal exposure were investigated by quantitative real-time RT-PCR. The bacterial challenge could significantly up-regulate the mRNA expression, and the highest expression level was detected at 24 h post-infection with 6.0-fold increase compared with that in the control group. During heavy metal exposure experiment, the expression of VpHSP40 could also be induced by Cu(2+) and Cd(2+) at different concentration, where Cu(2+) displayed more toxic effect on clam than that of Cd(2+). Concerning the same heavy metal, varied effect on VpHSP40 expression was detected at different concentration of heavy metal. Taking together, these results suggested that VpHSP40 was perhaps involved in mediating the immune responses and environmental stresses in V. philippinarum.
Key Laboratory of Coastal Zone Environment Processes, CAS, Shandong Provincial Key Laboratory of Coastal Zone Environment Processes, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, 17 Chunhui Road, Laishan District, Yantai 264003, China.
Chenghua
C
Li
Lingyun
L
Li
Feng
F
Liu
Xuanxuan
X
Ning
Aiqin
A
Chen
Linbao
L
Zhang
Huifeng
H
Wu
Jianmin
J
Zhao
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D049872:Bivalvia / Q000378:metabolism* / Q000382:microbiology; D003001:Cloning, Molecular; D020224:Expressed Sequence Tags; D005786:Gene Expression Regulation / Q000187:drug effects* / Q000502:physiology; D050956:HSP40 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D054884:Host-Pathogen Interactions; D019216:Metals, Heavy / Q000633:toxicity*; D058977:Molecular Sequence Annotation; D014733:Vibrio / Q000502:physiology*
D050956:HSP40 Heat-Shock Proteins; D019216:Metals, Heavy