Study of the relationship between changes in lactic acid bacterial cell components and stimulation of IL-12 production under salt-stressed conditions.
One hundred and seventeen strains of plant origin lactic acid bacteria were observed to have interleukin (IL)-12 production-inducing activities using mouse peritoneal macrophages. Pediococcus pentosaceus (KKM122) was chosen for its stable and strong IL-12 production-inducing activity. There was no significant difference in IL-12 activity induced by the KKM122 strain grown in culture conditions of 0% and 6% NaCl. The cell wall components of cells grown in 6% salt condition, however, significantly induced lower IL-12 production as compared with those of cells grown in 0% salt condition. Cell wall components enhanced IL-12 activity by removing cytoplasmic components when KKM122 strain was cultured in 0% salt condition. The immunoenhancing factor was mainly present in the cell wall components. IL-12 production-inducing activities were dependent on both the amount of bacterial cytoplasmic components and the structure of the cell wall components under the NaCl concentration in the culture medium.
Research and Development Division, Kikkoman Corporation, Noda, Chiba, Japan. t-igarashi@mail.kikkoman.co.jp
Toshinori
T
Igarashi
D000818:Animals; D002473:Cell Wall / Q000276:immunology; D003593:Cytoplasm / Q000737:chemistry; D018664:Interleukin-12 / Q000096:biosynthesis*; D007777:Lactobacillaceae / Q000378:metabolism*; D017737:Macrophages, Peritoneal / Q000382:microbiology*; D051379:Mice; D010944:Plants / Q000382:microbiology; D012965:Sodium Chloride / Q000494:pharmacology*; D013312:Stress, Physiological
D018664:Interleukin-12; D012965:Sodium Chloride