[A study on the characteristics of the membrane potassium channels in human non-small cell lung cancer cell].
BACKGROUND
To explore the character istics of K+ channel of human non-small cell lung cancer cells ( NSCLCC) .


METHODS
The characteristics of membrane potassium channels were detected in NSCLCC and benign pulmonary epithelium cells ( BEC) as control by whole cell patch clamp recording technique.


RESULTS
(1) The transmembrane K+ current in NSCLCC was voltage-dependent. The K+ current of squamous cell carcinoma was voltage-dependent and inactivating, and that of adenocarcinoma was also voltage-dependent, but not inactivating . The membrane capacitance of NSCLCC was ( 15. 35± 0. 65) pF. The membrane current density of NSCLCC was ( 121. 08± 8. 35) A/ F. The time constant (τ) of activating K+ current was 3.6-9.8 ms when TP was 50-90 mV. The K+ current could be blocked by K+ channel blocker, TEA. (2) The K+ current amplitude level, density and membrane capacitance of NSCLCC were all remarkably higher than those of BEC ( P < 0. 001) . The time constant of NSCLCC was significantly smaller than that of BEC ( P < 0. 001) .


CONCLUSIONS
(1) The transmembrane current of K+ channels in NSCLCC is significantly higher than that in benign pulmonary lesion, and the activity significantly increases. (2) Detection of K+ channels of lung cancer cell membrane is helpful to provide theoretical and experimental basis for exploring the signal transmission of oncogenesis, development, invasion and metastasis of human lung cancer.
Department of Thoracocardiac Surgery , West ChinaHospital , Sichuan University ( Former The First University Hospital of West China University of Medical Sciences) , Chengdu, Sichuan 610041, P. R . China.
T
T
Dai
Q
Q
Zhou
X
X
Zeng
J
J
He
Y
Y
Yang
C
C
Li
D
D
Ren
L
L
Liu
B
B
Liao