ASPicDB: a database of annotated transcript and protein variants generated by alternative splicing.
Alternative splicing is emerging as a major mechanism for the expansion of the transcriptome and proteome diversity, particularly in human and other vertebrates. However, the proportion of alternative transcripts and proteins actually endowed with functional activity is currently highly debated. We present here a new release of ASPicDB which now provides a unique annotation resource of human protein variants generated by alternative splicing. A total of 256,939 protein variants from 17,191 multi-exon genes have been extensively annotated through state of the art machine learning tools providing information of the protein type (globular and transmembrane), localization, presence of PFAM domains, signal peptides, GPI-anchor propeptides, transmembrane and coiled-coil segments. Furthermore, full-length variants can be now specifically selected based on the annotation of CAGE-tags and polyA signal and/or polyA sites, marking transcription initiation and termination sites, respectively. The retrieval can be carried out at gene, transcript, exon, protein or splice site level allowing the selection of data sets fulfilling one or more features settled by the user. The retrieval interface also enables the selection of protein variants showing specific differences in the annotated features. ASPicDB is available at http://www.caspur.it/ASPicDB/.
Biocomputing Group, University of Bologna, Bologna 40126, Italy.
Pier L
PL
Martelli
Mattia
M
D'Antonio
Paola
P
Bonizzoni
Tiziana
T
Castrignanò
Anna M
AM
D'Erchia
Paolo
P
D'Onorio De Meo
Piero
P
Fariselli
Michele
M
Finelli
Flavio
F
Licciulli
Marina
M
Mangiulli
Flavio
F
Mignone
Giulio
G
Pavesi
Ernesto
E
Picardi
Raffaella
R
Rizzi
Ivan
I
Rossi
Alessio
A
Valletti
Andrea
A
Zauli
Federico
F
Zambelli
Rita
R
Casadio
Graziano
G
Pesole
D017398:Alternative Splicing; D030541:Databases, Genetic; D005091:Exons; D014644:Genetic Variation; D006801:Humans; D020033:Protein Isoforms / Q000737:chemistry / Q000235:genetics; D011506:Proteins / Q000737:chemistry* / Q000235:genetics*; D012333:RNA, Messenger / Q000737:chemistry; D020539:Sequence Analysis, Protein; D014584:User-Computer Interface
D020033:Protein Isoforms; D011506:Proteins; D012333:RNA, Messenger