The activity of selected glycosidases in salivary gland tumors.
The monitoring of the patients after salivary gland tumors surgery is an important clinical issue. Still imperfect diagnostic procedures also remain a challenge for searching new sensitive and specific biomarkers of neoplastic processes in salivary glands. The aim of the presented study was an the assessment of the activity of HEX, with its isoforms HEX-A and HEX-B, GLU, GAL, MAN and FUC in salivary gland tumor tissues in comparison to a healthy salivary gland tissues taken during autopsy. A group of 42 patients with benign and malignant salivary gland tumors, aged 25-65 were examined. Fragments of salivary gland tumor tissue, fragments of healthy tissue removed during autopsy, blood serum and saliva were collected from patients with salivary gland tumors and healthy volunteers. In salivary gland tumor tissue the activity of HEX, HEX-A, HEX-B, GAL, FUC was considerably higher than in comparison to healthy salivary gland tissue and ascending trend of activity of GLU, MAN was also noticed. The activity of all lysosomal exoglycosidases in blood serum in patients with salivary gland tumors was considerably higher in comparison to healthy volunteers blood serum. The considerably higher activity of HEX, HEX-A, GLU, GAL, MAN, FUC and descending trend of activity of HEX-B were noticed in saliva of patients with salivary gland tumors in comparison to healthy volunteers. The assessment of HEX in blood serum and saliva of patients with salivary gland tumor can be possibly used in diagnostics and monitoring of salivary glands tumors.
Department of Oral Surgery, Medical University of Bialystok, Poland. bierc@interia.pl
Marcin
M
Bierc
Lukasz
L
Minarowski
Lukasz
L
Woźniak
Sylwia
S
Chojnowska
Malgorzata
M
Knas
Slawomir
S
Szajda
Krzysztof
K
Zwierz
D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood; D016022:Case-Control Studies; D005260:Female; D006026:Glycoside Hydrolases / Q000378:metabolism*; D054818:Hexosaminidase A / Q000097:blood / Q000378:metabolism; D054819:Hexosaminidase B / Q000097:blood / Q000378:metabolism; D006801:Humans; D007527:Isoenzymes / Q000097:blood / Q000378:metabolism; D008247:Lysosomes / Q000201:enzymology; D008297:Male; D008875:Middle Aged; D012468:Salivary Gland Neoplasms / Q000201:enzymology* / Q000378:metabolism* / Q000473:pathology; D012469:Salivary Glands / Q000201:enzymology; D012680:Sensitivity and Specificity; D044967:Serum / Q000201:enzymology
D014408:Biomarkers, Tumor; D007527:Isoenzymes; D006026:Glycoside Hydrolases; D054818:Hexosaminidase A; D054819:Hexosaminidase B