The presence of IL-17A and T helper 17 cells in experimental mouse brain tumors and human glioma.
BACKGROUND
Recently, CD4(+)IL-17A(+) T helper 17 (Th17) cells were identified and reported in several diseased states, including autoimmunity, infection and various peripheral nervous system tumors. However, the presence of Th17 in glia-derived tumors of the central nervous system has not been studied.


RESULTS
In this report, we demonstrate that mRNA expression for the Th17 cell cytokine IL-17A, as well as Th17 cells, are present in human glioma. The mRNA expression for IL-17A in glioma was recapitulated in an immunocompetent mouse model of malignant glioma. Furthermore, the presence of Th17 cells was confirmed in both human and mouse glioma. Interestingly, some Th17 cells present in mouse glioma co-expressed the Th1 and Th2 lineage markers, IFN-γ and IL-4, respectively, but predominantly co-expressed the Treg lineage marker FoxP3.


CONCLUSIONS
These data confirm the presence of Th17 cells in glia-derived CNS tumors and provide the rationale for further investigation into the role of Th17 cells in malignant glioma.
The Brain Tumor Center, The University of Chicago, Chicago, Illinois, United States of America.
Derek A
DA
Wainwright
Sadhak
S
Sengupta
Yu
Y
Han
Ilya V
IV
Ulasov
Maciej S
MS
Lesniak
D000818:Animals; D001483:Base Sequence; D001932:Brain Neoplasms / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D017931:DNA Primers; D005455:Fluorescent Antibody Technique; D005910:Glioma / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D020381:Interleukin-17 / Q000378:metabolism*; D051379:Mice; D016133:Polymerase Chain Reaction; D006377:T-Lymphocytes, Helper-Inducer / Q000378:metabolism*
D017931:DNA Primers; D020381:Interleukin-17