Identification of source of calcium in HeLa cells by femtosecond laser excitation.
Calcium is an important messenger in cells and whose store and diffusion dynamics at the subcellular level remain unclear. By inducing a controlled slow subcellular Ca2+ release through femtosecond laser irradiation in HeLa cells immersed in different media, cytoplasm is identified to be the major intracellular Ca2+ store, with the nucleus being the minor store and the extracellular Ca2+ also contributing to the total cellular Ca2+ level. Furthermore, Ca2+ released in either the cytoplasm or nucleus diffuses into the nucleus or cytoplasm, respectively, at different rates and influences the Ca2+ release in those regions.
Chinese University of Hong Kong, Department of Electronic Engineering, Hong Kong.
Hao
H
He
Siu Kai
SK
Kong
Kam Tai
KT
Chan
D000814:Aniline Compounds; D002118:Calcium / Q000378:metabolism*; D002121:Calcium Channel Blockers / Q000494:pharmacology; D020013:Calcium Signaling / Q000528:radiation effects; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism / Q000528:radiation effects; D003593:Cytoplasm / Q000378:metabolism / Q000528:radiation effects; D005110:Extracellular Space / Q000378:metabolism / Q000528:radiation effects; D005456:Fluorescent Dyes; D006367:HeLa Cells; D006801:Humans; D007834:Lasers; D018613:Microscopy, Confocal; D009543:Nifedipine / Q000494:pharmacology; D055620:Optical Phenomena; D010775:Photic Stimulation; D014966:Xanthenes
D000814:Aniline Compounds; D002121:Calcium Channel Blockers; C409648:Fluo 4; D005456:Fluorescent Dyes; D014966:Xanthenes; D009543:Nifedipine; D002118:Calcium