OXA-23-producing Acinetobacter baumannii: a new hotspot of diversity in Rio de Janeiro?
OBJECTIVE
this study focused on the population structure of OXA-23-producing Acinetobacter baumannii clinical isolates from Rio de Janeiro, Brazil.


METHODS
the analysis included several genomic typing methods, including PFGE, two multilocus sequence typing (MLST) schemes, sequence group (SG) determination and bla(OXA-51-like) sequencing. The genomic context of the bla(OXA-23) gene was also evaluated using I-CeuI hybridizations and PCR assays.


RESULTS
congruent clustering was obtained revealing four lineages. In accordance, four new sequence types (STs) (ST131, ST132, ST133 and ST134) were obtained with the MLST-OD scheme (associated with the Oxford Database) and four (ST79, ST15 and two new allelic profiles) with the MLST-IP scheme (developed by the Institute Pasteur). Four SGs (SG1, SG4 and two new profiles) were identified, allowing the association of 70% of the isolates with European clone II. bla(OXA-51-like) sequencing revealed the presence of bla(OXA-66), bla(OXA-69), bla(OXA-95) and bla(OXA-132).


CONCLUSIONS
identification of new STs together with new SG profiles are findings suggestive of a local diversity hotspot that is worth exploring.
REQUIMTE, Laboratório de Microbiologia, Faculdade de Farmácia, Universidade do Porto, Porto, Portugal.
Filipa
F
Grosso
Karyne Rangel
KR
Carvalho
Sandra
S
Quinteira
Ana
A
Ramos
Ana Paula D'Alincourt
AP
Carvalho-Assef
Marise Dutra
MD
Asensi
Luísa
L
Peixe
D000151:Acinetobacter Infections / Q000453:epidemiology* / Q000382:microbiology*; D040981:Acinetobacter baumannii / Q000145:classification* / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001938:Brazil / Q000453:epidemiology; D016000:Cluster Analysis; D016521:Electrophoresis, Gel, Pulsed-Field; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D017720:Molecular Epidemiology; D058889:Molecular Typing; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D001618:beta-Lactamases / Q000096:biosynthesis* / Q000235:genetics*
C466679:beta-lactamase OXA-23; D001618:beta-Lactamases