[Genotype distribution of enterovirus group C in Shandong Province, China].
Human Enterovirus C group (HEV-C) includes 17 serotypes, which can not be serotype-identified by neutralization test using antiserum pool for NPEV. In order to elucidate the genotypes and molecular evolution of HEV-C in Shandong Province, We selected the strains isolated from AFP cases between 1994-2009 to perform reverse transcriptase-polymerase chain reactions (RT-PCR) by the primers specific for entire VP1 coding gene of HEV-C and sequencing. The phylogenetic tree was then constructed among these VP1 nucleotide sequences and other prototype strains. Totally 12 Shandong local strains were obtained and separated into 4 genotypes, CVA20, CVA21,CVA24 and EV 96. The homologous comparison and phylogenetic analysis showed Shandong strains were distinct from prototype strains in each genotype. This report showed that different genotype HEV-C strains spread widely in Shandong Province.
Shandong Center for Disease Control and Prevention, Shandong Provincial Key Laboratory of Infectious Disease Control and Prevention, Jinan 250014, China. liyan7516@163.com
Yan
Y
Li
Yao
Y
Liu
Hai-yan
HY
Wang
Ze-xin
ZX
Tao
Li-zhi
LZ
Song
Gui-fang
GF
Liu
Lei
L
Feng
Yi
Y
Feng
Xiao-juan
XJ
Lin
He
H
Yang
Qing-ying
QY
Fan
Ai-qiang
AQ
Xu
D002460:Cell Line; D002681:China; D030015:Enterovirus C, Human / Q000145:classification / Q000235:genetics*; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction