Effects of temperature variations and light exposure on the time to growth of dermatophytes using six different fungal culture media inoculated with laboratory strains and samples obtained from infected cats.
In this study, 5/6 commercially available fungal culture media were comparable with respect to first growth, first colour change, and first sporulation when inoculated with three strains of Microsporum canis, one strain of Microsporum gypseum, and one strain of Trichophyton species when incubated at either 25°C or 30°C. Five of six plates showed 100% growth at both temperatures. Five of six plates showed 100% growth when inoculated with naturally infective M canis hairs and spores. One commercial product packaged as a self-sealing incubation plate showed growth in only 65.4% of times and the plates were prone to desiccation. M canis inoculated plates were incubated under four different light exposures (24h of light, 24h of dark, 12h light/12h dark, and room lighting) and no differences in growth or sporulation were noted.
University of Wisconsin-Madison, School of Veterinary Medicine, 2015 Linden Drive West, Madison, WI 53706, USA. moriellk@svm.vetmed.wisc.edu
Karen A
KA
Moriello
Maria J
MJ
Verbrugge
Rachel A
RA
Kesting
D000818:Animals; D002371:Cat Diseases / Q000382:microbiology; D002415:Cats; D003470:Culture Media; D003881:Dermatomycoses / Q000662:veterinary; D008027:Light; D008864:Microsporum / Q000145:classification / Q000254:growth & development*; D013696:Temperature; D013997:Time Factors; D014249:Trichophyton / Q000145:classification / Q000254:growth & development*
D003470:Culture Media