Application of sugaring-out extraction for the determination of sulfonamides in honey by high-performance liquid chromatography with fluorescence detection.
A simple sugaring-out assisted liquid-liquid extraction method combined with high-performance liquid-chromatography with fluorescence detection (HPLC-FL) was developed for the extraction and determination of sulfonamides in honey. Sample preparation consisted of acid hydrolysis to release sugar-bound sulfonamides. After derivatization with fluorescamine, the derivatives were partitioned into the organic layer under the honey (sugar)/water/acetonitrile system. The clear organic extract obtained by centrifugation could be injected into the HPLC system either directly or after dilution. Linearity was obtained with the coefficient of determination (R(2)) higher than 0.998 from 2 to 200 ng/mL. Under the optimal conditions, recoveries were determined for honey fortified at three levels (5, 20, and 100 ng/g) were 80.9-99.6% with coefficients of variation of 0.3-4.4%. Limits of detection for the sulfonamides studied were found to range from 0.6 to 0.9 ng/g.
The Health Bureau of Kaohsiung County Government, No. 834-1, Chengcing Road, Niaosong, Kaohsiung County 833, Taiwan.
Wen-Hsien
WH
Tsai
Hung-Yi
HY
Chuang
Ho-Hsien
HH
Chen
Yuh-Wern
YW
Wu
Shou-Hsun
SH
Cheng
Tzou-Chi
TC
Huang
D000097:Acetonitriles / Q000737:chemistry; D005591:Chemical Fractionation / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005450:Fluorescamine / Q000737:chemistry; D006722:Honey / Q000032:analysis*; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013449:Sulfonamides / Q000032:analysis* / Q000737:chemistry
D000097:Acetonitriles; D013449:Sulfonamides; D005450:Fluorescamine; C032159:acetonitrile