Production of tissue-engineered skin and oral mucosa for clinical and experimental use.
Since the early 1990s, our understanding of how epithelial and stromal cells interact in 3D tissue-engineered constructs has led to tissue-engineered skin and oral mucosa models, which are beginning to deliver benefit in the clinic (usually in small-scale reconstructive surgery procedures) but have a great deal to offer for in vitro investigations. These 3D tissue-engineered models can be used for a wide variety of purposes such as dermato- and mucotoxicity, wound healing, examination of pigmentation and melanoma biology, and in particular, a recent development from this laboratory, as a model of bacterially infected skin. Models can also be used to investigate specific skin disease processes. In this chapter, we describe the basic methodology for producing 3D tissue-engineered skin and oral mucosa based on de-epidermised acellular human dermis, and we give examples of how these models can be used for a variety of applications.
Department of Materials Science and Engineering, Kroto Research Institute, University of Sheffield, Sheffield, UK.
Sheila
S
MacNeil
Joanna
J
Shepherd
Louise
L
Smith
D001424:Bacterial Infections / Q000473:pathology; D001681:Biological Assay; D002469:Cell Separation; D002478:Cells, Cultured; D000078404:Epidermal Cells; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D015603:Keratinocytes / Q000166:cytology; D008954:Models, Biological; D009061:Mouth Mucosa / Q000166:cytology / Q000502:physiology*; D012867:Skin / Q000382:microbiology / Q000473:pathology; D012880:Skin Pigmentation / Q000502:physiology; D017768:Skin, Artificial; D013242:Sterilization; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds; D014945:Wound Healing