[Extraction and purification method of rice DNA from rice powder containing Konjak flour].
Rice powder containing Konjak flour made with tuberous roots of Amorphophallus konjac is imported as a rice-processed product from China to Japan. An improved DNA purification method for the polymerase chain reaction (PCR) analysis of rice in such products is necessary, since Konjak flour constituents absorb the DNA purification buffer to form a gel, and cause problems in the subsequent purification steps. Here, we present a simple preparative system for isolation of the rice and a purification method of the rice DNA from the product. The purified DNA was confirmed to be a good template for both PCR and real-time PCR.
Japan Inspection Association of Food and Food Industry Environment, 2-10-3 Shinkiba, Koutou-ku, Tokyo, Japan.
Kazuhiko
K
Minematsu
Kosuke
K
Nakamura
Hiroshi
H
Akiyama
Naoki
N
Harikai
Osamu
O
Nakajima
Kazumi
K
Kitta
Reiko
R
Teshima
Tayoshi
T
Iizuka
D031056:Amorphophallus; D018744:DNA, Plant / Q000302:isolation & purification*; D005504:Food Analysis / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D030841:Food, Genetically Modified; D012275:Oryza / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011208:Powders
D018744:DNA, Plant; D011208:Powders