[The application of quantitative detection of cystatin C in evaluation of renal function after renal transplantation].
OBJECTIVE
To establish ELISA method for quantitate the concentration of cystatin C (cys C) and to monitor the renal function of patients before and after renal transplantation.


METHODS
Hybridomas secreting monoclonal antibodies (mAbs) against human cys C were produced and sandwich ELISA kit for quantitatively detecting cys C was established. Then the concentrations of serum cystatin C (Scys C) and urine cystatin C (Ucys C) from normal controls and 23 patients undergoing renal transplantation were detected and their relationship with serum creatinine (SCR) was analyzed.


RESULTS
Seven hybridomas secreting anti-cys C mAbs were obtained. The sensitivity of the established ELISA kit reached 0.1 μg/L. The concentrations of Scys C and Ucys C of normal healthy controls were in accordance with other report. High correlations between Scys C or Ucys C and the level of SCR were observed (P<0.01). Rapid decline of Scys C and Ucys C concentrations was consistent with the decrease of SCR in the patients with normal course (NC) recovery after renal transplantation. However, Ucys C kept higher level within two weeks after the operation in patients with AR until the day 21. In patients with DGF, higher levels of Scys C, Ucys C and SCR were sustained within four weeks after renal transplantation.


CONCLUSIONS
The sensitive ELISA kit for detection of cys C has been established. Importantly, there are the persistently high levels of Scys C and Ucys C in patients with AR or DGF, which can be used as a novel indicator for monitoring renal function after renal transplantation.
Department of Immunology, the Fourth Military Medical University, Xi'an 710032, China.
Ying
Y
Ma
Xiao-guang
XG
Xu
Hai-yan
HY
Huang
Qi
Q
Li
Chao-jun
CJ
Song
Bing-yi
BY
Shi
Bo-quan
BQ
Jin
D000818:Animals; D003404:Creatinine / Q000097:blood; D055316:Cystatin C / Q000032:analysis* / Q000097:blood / Q000652:urine; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005919:Glomerular Filtration Rate; D006801:Humans; D007677:Kidney Function Tests / Q000379:methods*; D016030:Kidney Transplantation; D051379:Mice; D008807:Mice, Inbred BALB C
D055316:Cystatin C; D003404:Creatinine