Preliminary study on the freeze-drying of human bone marrow-derived mesenchymal stem cells.
Long-term preservation and easy transportation of human bone marrow-derived mesenchymal stem cells (hBM-MSCs) will facilitate their application in medical treatment and bioengineering. A pilot study on the freeze-drying of hBM-MSCs was carried out. hBM-MSCs were loaded with trehalose. The glass transition temperature of the freeze-drying suspension was measured to provide information for the cooling and primary drying experiment. After freeze-drying, various rehydration processes were tested. The highest recovery rate of hBM-MSCs was (69.33±13.08)%. Possible methods to improve freeze-drying outcomes are discussed. In conclusion, the present study has laid a foundation for the freeze-drying hBM-MSCs.
Refrigeration and Cryogenic Engineering Institute, Zhejiang University, Hangzhou, China.
Shao-zhi
SZ
Zhang
Huan
H
Qian
Zhen
Z
Wang
Ju-li
JL
Fan
Qian
Q
Zhou
Guang-ming
GM
Chen
Rui
R
Li
Shan
S
Fu
Jie
J
Sun
D001854:Bone Marrow Cells / Q000166:cytology* / Q000378:metabolism*; D018929:Cell Culture Techniques / Q000379:methods*; D002470:Cell Survival; D002478:Cells, Cultured; D005612:Freeze Drying / Q000379:methods*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism*; D010865:Pilot Projects; D014199:Trehalose / Q000493:pharmacokinetics*
D014199:Trehalose