New taxa in Alphaproteobacteria: Brevundimonas olei sp. nov., an esterase-producing bacterium.
A polyphasic taxonomic approach was used to characterize a Gram-negative, non-motile bacterium, designated MJ15(T), that was isolated from soil of a GS-Caltex Oil reservoir in Korea. As shown by comparative 16S rRNA gene sequence analysis, strain MJ15(T) belongs to genus Brevundimonas. The 16S rRNA gene sequence similarities ranged from 95.6-99.2% between strain MJ15(T) and validated representatives of the genus Brevundimonas. With respect to Brevundimonas species, strain MJ15(T) exhibited DNA-DNA relatedness values below 40.7%. The G+C content of the genomic DNA was 61.7 mol%. Strain MJ15(T) contained ubiquinone Q-10. The major fatty acids were C(16:0) (27.7%), C(19:0) cyclo ω8c (23.2%), summed feature 8 (containing C(18:1) ω7c/C(18:1) 6c) (28.5%), and major hydroxyl fatty acid was C(12:0) 3OH (3.7%). Based upon its phenotypic and genotypic properties, as well as its phylogenetic distinctiveness, strain MJ15(T) (KCTC 22461(T); JCM 16237(T)) should be classified in the genus Brevundimonas as the type strain of a novel species. The name Brevundimonas olei sp. nov. is proposed for this new species.
Research and Development Division, H-Plus Eco Ltd, BVC #301, KRIBB, Daejeon 305-333, Republic of Korea.
Myungjin
M
Lee
Sathiyaraj
S
Srinivasan
Myung Kyum
MK
Kim
D015373:Bacterial Typing Techniques; D001482:Base Composition; D042063:Caulobacteraceae / Q000145:classification* / Q000201:enzymology* / Q000235:genetics / Q000502:physiology; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004950:Esterases / Q000096:biosynthesis*; D005227:Fatty Acids / Q000032:analysis; D007723:Korea; D008124:Locomotion; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D014451:Ubiquinone / Q000032:analysis
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; D004950:Esterases; C025204:Ubiquinone Q2