Sec16A defines the site for vesicle budding from the endoplasmic reticulum on exit from mitosis.
Mitotic inhibition of COPII-dependent export of proteins from the endoplasmic reticulum results in disassembly of the Golgi complex. This ensures ordered inheritance of organelles by the two daughter cells. Reassembly of the Golgi is intimately linked to the re-initiation of ER export on exit from mitosis. Here, we show that unlike all other COPII components, which are cytosolic during metaphase, Sec16A remains associated with ER exit sites throughout mitosis, and thereby could provide a template for the rapid assembly of functional export domains in anaphase. Full assembly of COPII at exit sites precedes reassembly of the Golgi in telophase.
Cell Biology Laboratories, School of Biochemistry, Medical Sciences Building, University of Bristol, University Walk, Bristol, BS8 1TD, UK.
Helen
H
Hughes
David J
DJ
Stephens
D022162:Cytoplasmic Vesicles / Q000235:genetics / Q000378:metabolism*; D004721:Endoplasmic Reticulum / Q000235:genetics / Q000378:metabolism*; D006056:Golgi Apparatus / Q000235:genetics / Q000378:metabolism; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D008938:Mitosis; D021381:Protein Transport; D033921:Vesicular Transport Proteins / Q000235:genetics / Q000378:metabolism*
C517555:SEC16A protein, human; D033921:Vesicular Transport Proteins