Amelioration of tamoxifen-induced liver injury in rats by grape seed extract, black seed extract and curcumin.
Liver injury was induced in female rats using tamoxifen (TAM). Grape seeds (Vitis vinifera) extract (GSE), black seed (Nigella sativa) extract (NSE), curcumin (CUR) or silymarin (SYL) were orally administered to TAM-intoxicated rats. Liver histopathology of TAM-intoxicated:rats showed pathological changes. TAM-intoxication elicited declines in liver antioxidant enzymes levels (glutathione peroxidase, glutathione reductase, superoxide dismutase and catalase), reduced glutathione (GSH) and GSH/GSSG ratio plus the hepatic elevations in lipid peroxides, oxidized glutathione (GSSG), tumor necrosis factor-alpha (TNF-alpha) and serum liver enzymes; alanine transaminase, aspartate transaminase, alkaline phosphatase, lactate dehydrogenase and gamma glutamyl transferase levels. Oral intake of NSE, GSE, CUR or SYL to TAM-intoxicated rats, attenuated histopathological changes and corrected all parameters mentioned above. Improvements were prominent in case of NSE (similarly SYL) > CUR > GSE. Data indicated that NSE, GSE or CUR act as free radicals scavengers and protect TAM-induced liver injury in rats.
Medical Laboratories Technology Dept., Facuty of Applied Medical Sciences, Taibah University, Al-Madinah Al-Munawwarah, Saudi Arabia. hesham_elbeshbishy@hotmail.com
Hesham A
HA
El-Beshbishy
Ahmed M
AM
Mohamadin
Ayman A
AA
Nagy
Ashraf B
AB
Abdel-Naim
D000284:Administration, Oral; D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D001219:Aspartate Aminotransferases / Q000097:blood; D002374:Catalase / Q000378:metabolism; D056486:Chemical and Drug Induced Liver Injury / Q000097:blood / Q000209:etiology / Q000517:prevention & control*; D003474:Curcumin / Q000008:administration & dosage / Q000494:pharmacology*; D005260:Female; D005978:Glutathione / Q000378:metabolism; D019803:Glutathione Disulfide / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D056604:Grape Seed Extract / Q000008:administration & dosage / Q000494:pharmacology; D008054:Lipid Peroxides / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D031881:Nigella sativa / Q000737:chemistry; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012639:Seeds / Q000737:chemistry*; D013482:Superoxide Dismutase / Q000378:metabolism; D013629:Tamoxifen / Q000633:toxicity; D014409:Tumor Necrosis Factor-alpha / Q000097:blood; D027843:Vitis / Q000737:chemistry
D000894:Anti-Inflammatory Agents, Non-Steroidal; D056604:Grape Seed Extract; D008054:Lipid Peroxides; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D013629:Tamoxifen; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D005978:Glutathione; D003474:Curcumin; D019803:Glutathione Disulfide