The role of the basic helix-loop-helix transcription factor Dec1 in the regulatory T cells.
Naturally occurring regulatory T (Treg) cells play a central role in the maintenance of immune homeostasis and in restraining the development of spontaneous inflammatory responses. However, the underlying mechanisms of Treg homeostasis remain incompletely understood. Of particular note, the IL-2Rα (CD25) is crucial for the homeostasis of Treg cells and the prevention of lymphoproliferative autoimmune disease. In this paper, we report that the basic helix-loop-helix transcription factor Dec1 is involved in the homeostasis of Treg cells and plays a role in their survival or expansion after adoptive transfer to lymphopenic recipients. Hence, it is crucial for the suppression of effector T cell-mediated inflammatory responses. Enforced expression of Dec1 upregulates CD25 expression during thymocyte development and increases the number of Treg cells in the periphery. Dec1 binds the transcription factor Runx1 and colocalizes with Runx1 in Treg cells. Specifically, we demonstrate that in Treg cells the Dec1/Runx1 complex binds to regulatory elements present in the Il-2rα locus. Collectively, these data show how Dec1 mechanistically acts in Treg cells.
Department of Developmental Biology, Research Institute for Radiation Biology and Medicine, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan.
Kazuko
K
Miyazaki
Masaki
M
Miyazaki
Yun
Y
Guo
Norimasa
N
Yamasaki
Masamoto
M
Kanno
Zen-ichiro
Z
Honda
Hideaki
H
Oda
Hiroshi
H
Kawamoto
Hiroaki
H
Honda
D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D002470:Cell Survival / Q000502:physiology; D050676:Core Binding Factor Alpha 2 Subunit / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D018398:Homeodomain Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D006706:Homeostasis / Q000502:physiology*; D053645:Interleukin-2 Receptor alpha Subunit / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D011485:Protein Binding / Q000235:genetics / Q000276:immunology; D050378:T-Lymphocytes, Regulatory; D015854:Up-Regulation / Q000502:physiology*
D051792:Basic Helix-Loop-Helix Proteins; D050676:Core Binding Factor Alpha 2 Subunit; D018398:Homeodomain Proteins; D053645:Interleukin-2 Receptor alpha Subunit; C491539:Bhlhe40 protein, mouse; C508018:Il2ra protein, mouse; C493726:Runx1 protein, mouse