Visualization of color anatomy and molecular fluorescence in whole-mouse cryo-imaging.
We developed multi-scale, live-time interactive visualization of color image data, including microscopic whole-mouse cryo-images serving many biomedical applications. Using true-color volume rendering, we interactively, selectively enhanced anatomy using feature detection. For example, to enhance red organs (vessels, liver, etc.) and internal surfaces, we computed a red feature from R/(R+G+B) and surface features from color/gray-scale gradients, respectively. For >70GB cryo-image volumes, we developed multi-resolution visualization, which provided low-resolution rendering of an entire mouse and zooming to organs, tissues, and cells. Fusions of fluorescence and color cryo-volumes uniquely showed biodistribution of metastatic and stem cells within an anatomical context.
Department of Biomedical Engineering, Case Western Reserve University, Cleveland, OH 44106, USA. mxg183@case.edu
Madhusudhana
M
Gargesha
Mohammed Q
MQ
Qutaish
Debashish
D
Roy
Grant J
GJ
Steyer
Michiko
M
Watanabe
David L
DL
Wilson
D000818:Animals; D003124:Colorimetry / Q000379:methods* / Q000662:veterinary*; D015925:Cryopreservation / Q000662:veterinary*; D007089:Image Enhancement / Q000379:methods; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D051379:Mice; D008856:Microscopy, Fluorescence / Q000379:methods* / Q000662:veterinary*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D051598:Whole Body Imaging / Q000379:methods* / Q000662:veterinary*