Simultaneous isolation of Ixodidae and bacterial (Borrelia spp.) genomic DNA.
Tick and tick-borne diseases have become widely distributed throughout the United States. As a result, the interest associated with tick allocation and the potential threat they may pose has increased. Efforts have expanded to understand biotic and abiotic factors which may influence tick/pathogen distribution. Thus, we have developed a procedure which allows the simultaneous isolation of both tick and bacterial DNA. Downstream applications are diverse; however, we describe the use of multiplex PCR to confirm the presence of spirochete DNA from tick samples. We suspect that this procedure is not limited to tick-bacteria systems and may be applied to a variety of arthropod-related endeavors.
College of Medicine, University of Kentucky, Lexington, Kentucky, USA.
Brandon L
BL
Jutras
Zhiwei
Z
Liu
Catherine A
CA
Brissette
D000818:Animals; D001898:Borrelia / Q000235:genetics*; D004247:DNA / Q000302:isolation & purification; D016678:Genome; D023281:Genomics / Q000379:methods*; D026863:Ixodidae / Q000235:genetics*
D004247:DNA