Effects of low molecular weight sulfated galactan fragments from Botryocladia occidentalis on the pharmacological and enzymatic activity of sPLA2 from Crotalus durissus cascavella.
Low molecular weight fragments of sulfated galactans (Boc-5 and Boc-10) from the red algae Botryocladia occidentalis significantly inhibited Crotalus durissus cascavella sPLA2 enzymatic activity. Equimolar ratios of sPLA2 to Boc-5 or Boc-10 resulted in allosteric inhibition of sPLA2. Under the conditions tested, we observed that both Boc-5 and Boc-10 strongly decreased edema, myonecrosis, and neurotoxicity induced by native sPLA2.
UNESP, Campus do Litoral Paulista, São Vicente, Brasil.
M H
MH
Toyama
D O
DO
Toyama
V M
VM
Torres
G C
GC
Pontes
W R L
WR
Farias
F R
FR
Melo
S C B
SC
Oliveira
F H R
FH
Fagundes
E B S
EB
Diz Filho
B S
BS
Cavada
D000818:Animals; D003435:Crotalid Venoms / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*; D017839:Crotalus / Q000378:metabolism; D004347:Drug Interactions; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy*; D005685:Galactans / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D007700:Kinetics; D008297:Male; D008407:Mast Cells / Q000187:drug effects*; D054497:Phospholipases A2, Secretory / Q000737:chemistry / Q000378:metabolism / Q000633:toxicity; D051381:Rats; D017208:Rats, Wistar; D000461:Rhodophyta / Q000737:chemistry*; D012867:Skin / Q000187:drug effects* / Q000473:pathology; D013329:Structure-Activity Relationship; D013431:Sulfates / Q000737:chemistry
D003435:Crotalid Venoms; D005685:Galactans; D013431:Sulfates; D054497:Phospholipases A2, Secretory