Production of human interferon alfa 2b in plants of Nicotiana excelsior by Agrobacterium-mediated transient expression.
Human interferon alpha2b gene was transiently expressed in Nicotiana excelsior plants. Fusion with N. plumbaginifolia calreticulin signal peptide for improved apoplast targeting and carrying out the expression under optimized conditions resulted in maximal interferon activity of 3.2 x 10(3) IU/g fresh weight (FW) with an average of 2.1 +/- 0.8 x 10(3) IU/g FW. It proves that N. excelsior is a suitable host for Agrobacterium-mediated transient expression of genes encoding physiologically active human proteins. The transient expression conditions optimized for GFP marker protein were confirmed to be preferable for hIFN alpha2b.
Institute of Cell Biology and Genetic Engineering NAS of Ukraine, Kiev 03680, Ukraine.
Y R
YR
Sindarovska
I M
IM
Gerasymenko
Y V
YV
Sheludko
Z M
ZM
Olevinskaya
N Y
NY
Spivak
N V
NV
Kuchuk
D000818:Animals; D001709:Biotechnology / Q000379:methods*; D002478:Cells, Cultured; D003588:Cytopathogenic Effect, Viral / Q000187:drug effects; D017930:Genes, Reporter; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000235:genetics; D006801:Humans; D000077190:Interferon alpha-2; D016898:Interferon-alpha / Q000096:biosynthesis* / Q000302:isolation & purification / Q000494:pharmacology; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D010957:Plasmids; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000302:isolation & purification / Q000494:pharmacology; D011994:Recombinant Proteins; D012231:Rhizobium / Q000235:genetics*; D013552:Swine; D014026:Nicotiana / Q000235:genetics* / Q000378:metabolism; D018116:Vesiculovirus / Q000187:drug effects
D000077190:Interferon alpha-2; D016898:Interferon-alpha; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins