[Study on the inhibitory effect of RNA interference on replication of dengue virus].
To investigate the inhibitory effect of RNA interference (RNAi) on dengue virus I (DENV-1) replication. Small interfering RNA (siRNA) against the PreM gene of dengue virus was synthesized and transfected into C6/36 cells with liposome, which was then attacked by DENV-1 virus. The antiviral effect of siRNA was evaluated by cytopathic effect (CPE), the cell survival rate measured by MTT, and virus RNA quantified by real-time RT-PCR. The results showed that after 7 days post infection of dengue virus, the transfected C6/36 cells showed less CPE. The cell survival rate of the transfected C6/36 cells increased by 2.26 fold, and the amount of virus RNA in the transfected cells was reduced by about 97.54% as well. These findings indicated that the siRNA could effectively inhibit dengue virus RNA replication, and protect C6/36 cells from viral attack, indicating its potential role in prevention and treatment of dengue fever.
Guangzhou Center for Disease Control and Prevention, Guangzhou 510080, China.
Jin-ya
JY
Yue
Xin-wei
XW
Wu
Ye-jian
YJ
Wu
Xiang-zhong
XZ
Li
Li-yun
LY
Jiang
Qiao-yan
QY
Li
Lei
L
Li
Xia
X
Yang
D000818:Animals; D002460:Cell Line; D003716:Dengue Virus / Q000235:genetics* / Q000502:physiology*; D034622:RNA Interference / Q000502:physiology*; D034741:RNA, Small Interfering / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014779:Virus Replication / Q000235:genetics / Q000502:physiology*
D034741:RNA, Small Interfering; D012367:RNA, Viral