The euryhaline yeast Debaryomyces hansenii has two catalase genes encoding enzymes with differential activity profile.
Debaryomyces hansenii is a spoilage yeast able to grow in a variety of ecological niches, from seawater to dairy products. Results presented in this article show that (i) D. hansenii has an inherent resistance to H2O2 which could be attributed to the fact that this yeast has a basal catalase activity which is several-fold higher than that observed in Saccharomyces cerevisiae under the same culture conditions, (ii) D. hansenii has two genes (DhCTA1 and DhCTT1) encoding two catalase isozymes with a differential enzymatic activity profile which is not strictly correlated with a differential expression profile of the encoding genes.
Biología Celular, Facultad de Ciencias, Universidad Nacional Autónoma de México, UNAM, Mexico, Mexico. claudia.segal@gmail.com
Claudia
C
Segal-Kischinevzky
Beatriz
B
Rodarte-Murguía
Victor
V
Valdés-López
Guillermo
G
Mendoza-Hernández
Alicia
A
González
Luisa
L
Alba-Lois
D000595:Amino Acid Sequence; D002374:Catalase / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D025141:Drug Resistance, Fungal; D020869:Gene Expression Profiling; D006861:Hydrogen Peroxide / Q000633:toxicity; D050296:Microbial Viability / Q000187:drug effects; D008969:Molecular Sequence Data; D008970:Molecular Weight; D004718:Saccharomycetales / Q000187:drug effects / Q000201:enzymology*
D006861:Hydrogen Peroxide; D002374:Catalase