Characterization of Paenibacillus curdlanolyticus intracellular xylanase Xyn10B encoded by the xyn10B gene.
The Paenibacillus curdlanolyticus xyn10B gene encoding a family-10 xylanase was cloned and expressed in Escherichia coli, and the recombinant enzyme rXyn10B was characterized. Immunological analysis suggested that Xyn10B is an intracellular enzyme. rXyn10B hydrolyzed birch-wood xylan and xylooligosaccharides to produce mainly xylobiose, suggesting that it is an endoxylanase. Its properties were significantly different from those of some homologous enzymes.
Applied Microbiology Laboratory, Graduate School of Bioresources, Mie University, Tsu, Japan.
Maya
M
Sudo
Makiko
M
Sakka
Tetsuya
T
Kimura
Khanok
K
Ratanakhanokchai
Kazuo
K
Sakka
D003001:Cloning, Molecular; D004187:Disaccharides; D043364:Endo-1,4-beta Xylanases / Q000302:isolation & purification / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics; D006868:Hydrolysis; D056507:Paenibacillus / Q000201:enzymology*; D014990:Xylans / Q000378:metabolism; D014995:Xylosidases / Q000302:isolation & purification / Q000378:metabolism
D004187:Disaccharides; D014990:Xylans; D014995:Xylosidases; D043364:Endo-1,4-beta Xylanases; C004173:xylobiose