Proteomic analysis of oxidative stress-induced neuronal cell death by using two-dimensional fluorescence difference gel electrophoresis.
Oxidative stress has been implicated in a number of neurological disorders, including cerebral ischemia and neuro-degenerative diseases. Comprehensive proteomic studies were carried out using an immortalized mouse hippocampal cell line, HT22, exhibiting oxidative stress-mediated cell death upon glutamate treatment. Two-dimensional fluorescence difference gel electrophoresis (2D DIGE) of subcellular organelle fractions revealed that significant numbers of proteins showed quantitative changes during HT22 cell death, among which a total of 51 proteins were identified by mass spectrometry. The identified proteins indicate that HT22 cell death occurs through perturbations in mitochondrial function, changes in translational elongation machinery, and translocation of proteins across subcellular organelles. This list of proteins may shed light on oxidative stress-mediated neuronal cell death.
Medical Proteomics Research Center, Korea Research Institute of Bioscience and Biotechnology, 50 Eon-dong, Daejeon, Republic of Korea.
Eun Young
EY
Kim
Tae-Sung
TS
Yoon
Young Jae
YJ
Bahn
Dae Gwin
DG
Jeong
Mi Rim
MR
Park
Sang J
SJ
Chung
Sung Goo
SG
Park
Byoung Chul
BC
Park
Sang Chul
SC
Lee
Seong Eon
SE
Ryu
Kwang-Hee
KH
Bae
D000818:Animals; D015153:Blotting, Western; D016923:Cell Death / Q000187:drug effects / Q000502:physiology*; D002461:Cell Line, Transformed; D003598:Cytoskeletal Proteins / Q000737:chemistry / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D018698:Glutamic Acid; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism; D051379:Mice; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology*; D010445:Peptide Elongation Factors / Q000737:chemistry / Q000378:metabolism; D020543:Proteome / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D013050:Spectrometry, Fluorescence
D003598:Cytoskeletal Proteins; D010445:Peptide Elongation Factors; D020543:Proteome; D018698:Glutamic Acid