[Study on the extraction of breviscapine from Erigeron breviscapus with ultrasonic wave technology optimized by central composite design-response surface method].
OBJECTIVE
To evaluate the effectiveness of ultrasound-assisted extraction and establish the optimized extraction conditions of breviscapine from Erigeron breviscapus.


METHODS
On the basis of the single factor and according to the central composite design principles (CCD), the response surface method (RSM) with 3 factors and 3 levels was adopted and the independent variables were ultrasonic wave extracting time, ethanol concentration, and the ratio of solvent volume to Erigeron breviscapus mass, breviscapine extraction yield determinated by HPLC was used as response value.


RESULTS
Ultrasonic wave extracting time of 24.5 min, ethanol concentration of 74.7% and the ratio of solvent volume to Erigeron breviscapus mass of 19.8 (mL/g) were selected as optimum conditions. Regression coefficients of binomial fitting complex model was 0. 9549 and the predicted breviscapine extraction yield was 0.641%, while the actual extraction yield was 0.632% (n = 5), with relative error of -1.14%.


CONCLUSIONS
This study confirms the efficiency of ultrasound-assisted extraction as a simple, inexpensive and effective method to improve the extraction of breviscapine from Erigeron breviscapus. The observed and predicted values are close to each other, which proves that the optimization of supersonic extraction process of breviscapine from Erigeron breviscapus by CCD-RSM is reasonable and successful.
Department of Chemical Science and Technology, Kunming University, Kunming 650031, China.
Mi-Er
ME
He
Wei
W
Wang
Li-Sheng
LS
Wu
Yun-Tao
YT
Gao
Shi-Yue
SY
Li
D000704:Analysis of Variance; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification; D049919:Erigeron / Q000737:chemistry*; D000431:Ethanol / Q000737:chemistry; D005419:Flavonoids / Q000302:isolation & purification*; D010946:Plants, Medicinal / Q000737:chemistry; D013678:Technology, Pharmaceutical / Q000379:methods*; D013997:Time Factors; D014465:Ultrasonics
D004365:Drugs, Chinese Herbal; D005419:Flavonoids; C061097:breviscapine; D000431:Ethanol