[The effects of in vitro culture conditions on regeneration of Fritillaria cirrhosa].
OBJECTIVE
The optimal in vitro culture conditions for the regeneration of Fritillaria cirrhosa were screened.


METHODS
Through the in vitro culture of the flowering stage Fritillaria cirrhosa, the effect of illumination, culture temperature, hormone combination and the amount of ribavirin on the regeneration of Fritillaria cirrhosa were studied.


CONCLUSIONS
The explant browning level could be apparently reduced if the bulb were cultured under the ray after exposure to proper low-temperature. The optimal temperature for the regeneration of bulb is (20 +/- 2) degrees C. The hormone has obvious promotional effect on the regeneration of bulb, and the best combination is 6-BA 2.0 mg/L plus NAA 0.2 mg/L Concentration of 10 mg/L of ribavirin is best for the regeneration of bulb.
Department of Biological Engineering, Chengdu University, Chengdu 610160, China. wangyaohua888@yahoo.com.cn
Yue-Hua
YH
Wang
Yong
Y
Dai
Zong-Sheng
ZS
He
Yan-Xia
YX
Sun
Sheng-Jie
SJ
Yan
Shi-Jun
SJ
Xu
Xiao-Rong
XR
Wang
D003247:Conservation of Natural Resources; D031410:Fritillaria / Q000254:growth & development* / Q000378:metabolism*; D008027:Light; D010937:Plant Growth Regulators / Q000737:chemistry / Q000378:metabolism*; D018517:Plant Roots / Q000254:growth & development* / Q000378:metabolism; D012038:Regeneration; D012254:Ribavirin / Q000737:chemistry / Q000378:metabolism*; D013696:Temperature; D046509:Tissue Culture Techniques / Q000379:methods
D010937:Plant Growth Regulators; D012254:Ribavirin