Expression and characterization of a novel mesophilic protease from metagenomic library derived from Antarctic coastal sediment.
A metagenomic cosmid library was constructed, in which the insert DNA was derived from the coastal sediment near Antarctic China Zhongshan Station. One clone (ACPRO001) expressing protease activity was isolated from the library using milk agar plates. Sequencing of the clone revealed a novel protease gene. The amino acid sequence comparison and phylogenetic analysis indicated that it could be classified as a subtilisin-like serine protease, though the highly conserved residue Asp was replaced by Ala. The ACPRO001 protease gene was expressed in pET-His and purified for characterization. The optimal temperature and pH for the activity of the ACPRO001 protease were 60°C and pH 9.0, respectively. The enzyme retained about 73% of residual activity after 2 h incubation at 50°C in the presence of Ca(2+). The presence of Ca(2+) increased the thermostability of ACPRO001 protease obviously. The enzymatic activity was inhibited by 1 mM phenylmethyl sulfonylfluoride (PMSF) and hydrochloride 4-(2-aminoethyl)-benzenesulfonyl fluoride (AEBSF), indicating that it was a serine protease.
Key Lab of Marine Biogenetic Resources, Third Institute of Oceanography, SOA, Daxue Road 178#, Xiamen 361005, China.
Yingjing
Y
Zhang
Jing
J
Zhao
Runying
R
Zeng
D001110:Arctic Regions; D002118:Calcium / Q000737:chemistry*; D003001:Cloning, Molecular; D004795:Enzyme Stability; D015870:Gene Expression; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D054892:Metagenome; D010447:Peptide Hydrolases / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification
D010447:Peptide Hydrolases; D002118:Calcium