Integrated biomarker assessment of the effects exerted by treated produced water from an onshore natural gas processing plant in the North Sea on the mussel Mytilus edulis.
The biological impact of a treated produced water (PW) was investigated under controlled laboratory conditions in the blue mussel, Mytilus edulis. Mussel health status was assessed using an integrated biomarker approach in combination with chemical analysis of both water (with SPMDs), and mussel tissues. Acyl-CoA oxidase activity, neutral lipid accumulation, catalase activity, micronuclei formation, lysosomal membrane stability in digestive cells and haemocytes, cell-type composition in digestive gland epithelium, and the integrity of the digestive gland tissue were measured after 5 week exposure to 0%, 0.01%, 0.1%, 0.5% and 1% PW. The suite of biomarkers employed were sensitive to treated PW exposure with significant sublethal responses found at 0.01-0.5% PW, even though individual chemical compounds of PW were at extremely low concentrations in both water and mussel tissues. The study highlights the benefits of an integrated biomarker approach for determining the potential effects of exposure to complex mixtures at low concentrations. Biomarkers were integrated in the Integrative Biological Response (IBR/n) index.
Norwegian Institute for Water Research (NIVA), Oslo, Norway. sbr@niva.no
Steven
S
Brooks
Christopher
C
Harman
Beñat
B
Zaldibar
Urtzi
U
Izagirre
Tormod
T
Glette
Ionan
I
Marigómez
D042962:Acyl-CoA Oxidase / Q000378:metabolism; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D004064:Digestive System / Q000187:drug effects; D004784:Environmental Monitoring; D005579:Fossil Fuels / Q000633:toxicity*; D006434:Hemocytes / Q000187:drug effects; D050356:Lipid Metabolism / Q000187:drug effects; D008247:Lysosomes / Q000187:drug effects; D009153:Mutagens / Q000633:toxicity; D049880:Mytilus edulis / Q000187:drug effects* / Q000378:metabolism; D009659:North Sea; D014865:Waste Disposal, Fluid; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D015415:Biomarkers; D005579:Fossil Fuels; D009153:Mutagens; D014874:Water Pollutants, Chemical; D002374:Catalase; D042962:Acyl-CoA Oxidase