Nanoparticles as fluorescent labels for optical imaging and sensing in genomics and proteomics.
Optical labelling reagents (dyes and fluorophores) are an essential component of probe-based biomolecule detection, an approach widely employed in a variety of areas including environmental analysis, disease diagnostics, pharmaceutical screening, and proteomic and genomic studies. Recently, functional nanomaterials, as a new generation of high-value optical labels, have been applied to molecular detection. The great potential of such recent optical labels has paved the way for the development of new biomolecule assays with unprecedented analytical performance characteristics, related to sensitivity, multiplexing capability, sample throughput, cost-effectiveness and ease of use. This review aims to provide an overview of recent advances using different nanoparticles (such as quantum dots, rare earth doped nanoparticles or gold nanoparticles) for analytical genomics and proteomics, with particular emphasis on the outlook for different strategies of using nanoparticles for bioimaging and quantitative bioanalytical applications, as well as possibilities and limitations of nanoparticles in such a growing field.
Department of Physical and Analytical Chemistry, Faculty of Chemistry, University of Oviedo, Julian Clavería, 8, 33006 Oviedo, Spain.
Ana María
AM
Coto-García
Emma
E
Sotelo-González
María Teresa
MT
Fernández-Argüelles
Rosario
R
Pereiro
José M
JM
Costa-Fernández
Alfredo
A
Sanz-Medel
D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D003952:Diagnostic Imaging / Q000295:instrumentation* / Q000379:methods; D005456:Fluorescent Dyes / Q000737:chemistry*; D023281:Genomics / Q000295:instrumentation* / Q000379:methods; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry*; D040901:Proteomics / Q000295:instrumentation* / Q000379:methods
D005456:Fluorescent Dyes