A simple colorimetric method for testing antimicrobial susceptibility of biofilmed bacteria.
This study introduces a simple colorimetric method which can measure the antimicrobial susceptibility of bacteria in biofilms using trimethyl tetrazolium chloride (TTC) as an indicator of viable bacteria. The new method was utilized for the evaluation of antibiotic susceptibility of Escherichia coli, Klebsiella pneumoniae, and Staphylococcus aureus biofilms.
Department of Microbiology, Kyungpook National University, School of Medicine, Daegu 700-422, Republic of Korea.
Shukho
S
Kim
Mi Jin
MJ
Kim
Hee Young
HY
Kang
Sung Yong
SY
Seol
Dong Taek
DT
Cho
Jungmin
J
Kim
D000890:Anti-Infective Agents / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development*; D003124:Colorimetry / Q000379:methods*; D004926:Escherichia coli / Q000187:drug effects* / Q000502:physiology; D005562:Formazans / Q000378:metabolism; D007711:Klebsiella pneumoniae / Q000187:drug effects* / Q000502:physiology; D008826:Microbial Sensitivity Tests / Q000379:methods; D050296:Microbial Viability / Q000187:drug effects; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000502:physiology; D013778:Tetrazolium Salts / Q000378:metabolism
D000890:Anti-Infective Agents; D005562:Formazans; D013778:Tetrazolium Salts