Attenuation of mouse melanoma by A/C magnetic field after delivery of bi-magnetic nanoparticles by neural progenitor cells.
Localized magnetic hyperthermia as a treatment modality for cancer has generated renewed interest, particularly if it can be targeted to the tumor site. We examined whether tumor-tropic neural progenitor cells (NPCs) could be utilized as cell delivery vehicles for achieving preferential accumulation of core/shell iron/iron oxide magnetic nanoparticles (MNPs) within a mouse model of melanoma. We developed aminosiloxane-porphyrin functionalized MNPs, evaluated cell viability and loading efficiency, and transplanted neural progenitor cells loaded with this cargo into mice with melanoma. NPCs were efficiently loaded with core/shell Fe/Fe(3)O(4) MNPs with minimal cytotoxicity; the MNPs accumulated as aggregates in the cytosol. The NPCs loaded with MNPs could travel to subcutaneous melanomas, and after A/C (alternating current) magnetic field (AMF) exposure, the targeted delivery of MNPs by the cells resulted in a measurable regression of the tumors. The tumor attenuation was significant (p < 0.05) a short time (24 h) after the last of three AMF exposures.
Department of Anatomy and Physiology, 228 Coles Hall, Kansas State University, Manhattan, Kansas 66506, USA.
Raja Shekar
RS
Rachakatla
Sivasai
S
Balivada
Gwi-Moon
GM
Seo
Carl B
CB
Myers
Hongwang
H
Wang
Thilani N
TN
Samarakoon
Raj
R
Dani
Marla
M
Pyle
Franklin O
FO
Kroh
Brandon
B
Walker
Xiaoxuan
X
Leaym
Olga B
OB
Koper
Viktor
V
Chikan
Stefan H
SH
Bossmann
Masaaki
M
Tamura
Deryl L
DL
Troyer
D000818:Animals; D001692:Biological Transport; D045744:Cell Line, Tumor; D004553:Electric Conductivity; D005260:Female; D005290:Ferric Compounds / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D007501:Iron / Q000737:chemistry / Q000378:metabolism; D055909:Magnetic Field Therapy / Q000379:methods*; D008545:Melanoma / Q000378:metabolism* / Q000473:pathology / Q000628:therapy*; D051379:Mice; D053758:Nanoparticles; D009420:Nervous System / Q000166:cytology*; D040901:Proteomics; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000378:metabolism*; D013696:Temperature
D005290:Ferric Compounds; C000499:ferric oxide; D007501:Iron