Local hyperthermia decreases the expression of CCL-20 in condyloma acuminatum.
BACKGROUND
Local hyperthermia has been successfully used in the treatment of viral warts. However, the mechanism of action has largely remained unclear. CCL-20 (also known as MIP-3α) is the most potent chemokine for recruitment of Langerhans cell (LC) precursors into the skin. CCL-20 expression can be increased by TNF-α and IL-1α. The effects of local hyperthermia on the mRNA expressions of CCL-20, TNF-α, IL-1α have been investigated in both condyloma acuminata (CA) and normal skin. Under an organotypic culture condition, fresh CA and normal skin were subjected to surface heating at 37°C, 42°C and 45°C for 30 mins, respectively.


RESULTS
The mRNA expressions of CCL-20 and IL-1α in CA specimen were significantly higher than those in normal skin. Local hyperthermia at 42°C and 45°C significantly decreased the mRNA levels of CCL-20 and IL-1α, as compared with the control groups (p < 0.01). The decrease of CCL-20 was well correlated with that of IL-1α. The expression of TNF-α in CA remained unchanged in spite of the temperature variation. Local hyperthermia at 45°C concomitantly increased the mRNA expression of CCL-20 and IL-1α in normal skin.


CONCLUSIONS
Our study suggests that hyperthermia decreases the expression of CCL-20 with concomitant decrease in IL-1α, and reduce the number of Langerhans cells in HPV infected skin.
Department of Dermatology, No,1 Hospital of China Medical University, Shenyang 110001, PR China.
Xiaoqin
X
Wang
Xing-Hua
XH
Gao
Yuxiao
Y
Hong
Xiaodong
X
Li
Hong-Duo
HD
Chen
D054418:Chemokine CCL20 / Q000096:biosynthesis*; D003218:Condylomata Acuminata / Q000276:immunology* / Q000473:pathology*; D015536:Down-Regulation; D005260:Female; D015870:Gene Expression; D006801:Humans; D006979:Hyperthermia, Induced; D053582:Interleukin-1alpha / Q000096:biosynthesis; D007801:Langerhans Cells / Q000276:immunology; D008297:Male; D012333:RNA, Messenger / Q000096:biosynthesis; D012867:Skin / Q000276:immunology / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
C108355:CCL20 protein, human; D054418:Chemokine CCL20; D053582:Interleukin-1alpha; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha