SOD/catalase mimetic platinum nanoparticles inhibit heat-induced apoptosis in human lymphoma U937 and HH cells.
Platinum nanoparticles (Pt-NPs) are known to possess anti-tumouric activity and the ability to scavenge superoxides and peroxides indicating that they can act as superoxide dismutase (SOD)/catalase mimetics. These potentials seem useful in the protection and/or amelioration of oxidative stress-associated pathologies, but, when they are combined with a therapeutic modality that depends upon the mediation of reactive oxygen species in cell killing induction, the effect of Pt-NPs might be questionable. Here, the effects of polyacrylic acid-capped Pt-NPs (nano-Pts) on hyperthermia (HT)-induced apoptosis and the underlying molecular mechanisms were investigated in human myelomonocytic lymphoma U937 and human cutaneous T-cell lymphoma HH cells. The results showed that the pre-treatment with nano-Pts significantly inhibited HT-induced apoptosis in a dose-dependent manner. Superoxide, but not peroxides, was suppressed to varying extents. All pathways involved in apoptosis execution were also negatively affected. The results reveal that the combination of nano-Pts and HT could result in HT-desensitization.
Department of Dermatology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, Japan.
Yoko
Y
Yoshihisa
Qing-Li
QL
Zhao
Mariame Ali
MA
Hassan
Zhang-Li
ZL
Wei
Megumi
M
Furuichi
Yusei
Y
Miyamoto
Takashi
T
Kondo
Tadamichi
T
Shimizu
D000180:Acrylic Resins; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D053148:Caspase 3 / Q000378:metabolism; D002374:Catalase / Q000378:metabolism*; D045744:Cell Line, Tumor; D053938:DNA Fragmentation; D005434:Flow Cytometry; D016166:Free Radical Scavengers / Q000737:chemistry; D006358:Hot Temperature; D006801:Humans; D008223:Lymphoma; D053758:Nanoparticles; D010545:Peroxides / Q000378:metabolism; D010984:Platinum / Q000737:chemistry* / Q000494:pharmacology* / Q000627:therapeutic use; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism*; D013481:Superoxides / Q000378:metabolism; D020298:U937 Cells
D000180:Acrylic Resins; D000970:Antineoplastic Agents; D016166:Free Radical Scavengers; D010545:Peroxides; D017382:Reactive Oxygen Species; D013481:Superoxides; D010984:Platinum; C006903:carbopol 940; D002374:Catalase; D013482:Superoxide Dismutase; D053148:Caspase 3