[Simultaneous determination of jatrorrhizine, palmatine, berberine, and obacunone in Phellodendri Amurensis Cortex by RP-HPLC].
OBJECTIVE
To develop a HPLC method to determine the content of jatrorrhizine, palmatine, berberine, and obacunone in Phellodendri Amurensis Cortex simultaneously. MEHTOD: The separations were carried out at 25 degrees C on a Phenomenex Gemini C18 column (4.6 mm x 250 mm, 5 microm) eluted with acetonitril and water containing 0.1% phosphoric acid in gradient mode. The flow rate was 1.0 mL x min(-1), detection wavelengthes were 345 nm for jatrorrhizine , palmatine, berberine and 210 nm for obacunone.


RESULTS
The average recoveries of jatrorrhizine, palmatine, berberine, and obacunone were 98.94%, 101.17%, 96.22% and 98.90%, respectively.


CONCLUSIONS
The method is simple, accurate and repeatable, and can be used in content determination of jatrorrhizine, palmatine, berberine, and obacunone in Phellodendri Amurensis Cortex.
National Engineering Laboratory for Propagation of Endangered Traditional Chinese Medicine, Chongqing Academy of Chinese Materia Medica, Chongqing 400065, China.
Qian
Q
Zhang
Lifen
L
Cai
Guoyue
G
Zhong
Weizao
W
Luo
D001584:Benzoxepins / Q000032:analysis*; D001599:Berberine / Q000031:analogs & derivatives* / Q000032:analysis*; D001600:Berberine Alkaloids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D036701:Limonins / Q000032:analysis*; D032102:Phellodendron / Q000737:chemistry*
D001584:Benzoxepins; D001600:Berberine Alkaloids; D004365:Drugs, Chinese Herbal; D036701:Limonins; C055785:jatrorrhizine; D001599:Berberine; C067207:obacunone; C005413:palmatine