Transfer of the barrier to radial oxygen loss in roots of Hordeum marinum to wheat (Triticum aestivum): evaluation of four H. marinum-wheat amphiploids.
• Wide hybridization of waterlogging-tolerant Hordeum marinum with wheat (Triticum aestivum) to produce an amphiploid might be one approach to improve waterlogging tolerance in wheat. • Growth, root aerenchyma and porosity, and radial oxygen loss (ROL) along roots were measured in four H. marinum-wheat amphiploids and their parents (four accessions of H. marinum and Chinese Spring wheat) in aerated or stagnant nutrient solution. A soil experiment was also conducted. • Hordeum marinum maintained shoot dry mass in stagnant nutrient solution, whereas the growth of wheat was markedly reduced (40% of aerated control). Two of the four amphiploids were more tolerant than wheat (shoot dry masses of 59-72% of aerated controls). The porosity of adventitious roots when in stagnant solution was higher in H. marinum (19-25%) and the four amphiploids (20-24%) than in wheat (16%). In stagnant solution, adventitious roots of H. marinum formed a strong ROL barrier in basal zones, whereas, in wheat, the barrier was weak. Two amphiploids formed a strong ROL barrier and two formed a moderate barrier when in stagnant solution. • This study demonstrates the transfer of higher root porosity and a barrier to ROL from H. marinum to wheat through wide hybridization and the production of H. marinum-wheat amphiploids.
School of Plant Biology, The University of Western Australia, Crawley, WA 6009, Australia.
A I
AI
Malik
A K M R
AK
Islam
T D
TD
Colmer
D018533:Biomass; D015687:Cell Hypoxia; D003470:Culture Media / Q000494:pharmacology; D001467:Hordeum / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D006824:Hybridization, Genetic / Q000187:drug effects; D010100:Oxygen / Q000378:metabolism* / Q000494:pharmacology; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D018520:Plant Shoots / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D016062:Porosity / Q000187:drug effects; D012987:Soil; D012996:Solutions / Q000494:pharmacology; D014908:Triticum / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*
D003470:Culture Media; D012987:Soil; D012996:Solutions; D010100:Oxygen