Production of monoclonal antibody against Salmonella H: g,m flagellar antigen and potential diagnostic application.
In this study, the flagella antigen was detached at high speed by shaking vigorously with glass pearl beads, from Salmonella enteritidis in a yield of 3.9 mg/mL(-1) after being concentrated with polyethylene glycol (PEG). A monoclonal antibody (MAb), designated D7 clone, was generated from Balb/c mice immunized with Salmonella enteritidis flagella using the conventional hybridoma method. The D7 clone secreting MAb was classified as IgG2a isotype. ELISA analyses of D7 MAb to Salmonella-specific flagella demonstrated that the antibody reacted with H: m. However, Western immunoblot analyses of D7 clone appear to be secreting heavy chain of IgG2a antibody, which was eligible for the diagnosis of Salmonella enteritidis.
Faculty of Engineering, Bioengineering Department, Ege University, Bornova, Izmir, Turkey.
Ayse
A
Nalbantsoy
Ismail
I
Karaboz
Ismet Deliloglu
ID
Gurhan
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D000918:Antibody Specificity; D000942:Antigens, Bacterial / Q000276:immunology*; D019937:Diagnostic Techniques and Procedures; D004797:Enzyme-Linked Immunosorbent Assay; D005407:Flagella / Q000276:immunology; D005408:Flagellin / Q000276:immunology*; D006825:Hybridomas / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D012480:Salmonella Infections / Q000175:diagnosis*; D012477:Salmonella enteritidis / Q000276:immunology*; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D005408:Flagellin