Impedance-based flow cytometry for the measurement of microparticles.
Over the last several years, there has been considerable interest in evaluating the biological relevance of alterations in blood-borne microparticle populations. The most commonly employed technique for the characterization of microparticles is light scatter flow cytometry. However, the enumeration and sizing of submicron particles based on light scattering properties can be problematic. Impedance-based flow cytometry based on the Coulter principle offers a sensitive methodology to characterize microparticles. This review details the rationale for employing impedance-based flow cytometry in the measurement of blood-borne microparticles.
Division of Hematology-Oncology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, USA. jzwicker@bidmc.harvard.edu
Jeffrey I
JI
Zwicker
D000818:Animals; D001773:Blood Cells / Q000648:ultrastructure; D055252:Cell-Derived Microparticles; D017097:Electric Impedance; D005434:Flow Cytometry / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D010316:Particle Size; D012542:Scattering, Radiation