[Oligonucleotide microarray for subtyping of influenza virus A neuraminidase].
Microarray for influenza A neuraminidase subtyping was presented. Selection of oligoprobes proceeded in two steps. First step included selection of peptides specific for each subtype of neuraminidase. At the second step oligoprobes were calculated using found peptides structures with the subsequent additional selection of the most specific and representative probes. From 19 to 24 probes were used for determination of each subtype of neuraminidase. Microchip testing for 19 samples with the most widespread types (N1 and N2) specifies in unequivocal definition 18 of them and only one isolate has not been identified.
V A
VA
Riabinin
E V
EV
Kostina
A A
AA
Neverov
G A
GA
Maksakova
A N
AN
Siniakov
D000595:Amino Acid Sequence; D017931:DNA Primers; D006801:Humans; D009980:Influenza A virus / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification*; D007251:Influenza, Human / Q000821:virology*; D008969:Molecular Sequence Data; D009439:Neuraminidase / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction
D017931:DNA Primers; D015345:Oligonucleotide Probes; D009439:Neuraminidase