Instruments and methods in proteomics.
In the past decade, major developments in instrumentation and methodology have been achieved in proteomics. For proteome investigations of complex biological samples derived from cell cultures, tissues, or whole organisms, several techniques are state of the art. Especially, many improvements have been undertaken to quantify differences in protein expression between samples from, e.g., treated vs. untreated cells and healthy vs. control patients. In this review, we give a brief insight into the main techniques, including gel-based protein separation techniques, and the growing field of mass spectrometry.
Department of Functional Proteomics, Medizinisches Proteom-Center, Ruhr-Universität Bochum, Bochum, Germany.
Caroline
C
May
Frederic
F
Brosseron
Piotr
P
Chartowski
Cornelia
C
Schumbrutzki
Bodo
B
Schoenebeck
Katrin
K
Marcus
D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D013058:Mass Spectrometry; D011506:Proteins / Q000032:analysis; D040901:Proteomics / Q000295:instrumentation* / Q000379:methods*
D011506:Proteins