[Genetic mechanisms of Salmonella enteritidis biodiversity and clinical features of salmonellosis].
OBJECTIVE
To assess prevalence of fragments of Escherichia coli pathogenicity islands in Salmonella enteritidis strains as well as to study clinical signs of disease caused by these strains in adults.


METHODS
Ninety-six patients with salmonellosis were studied. Ninety strains of S. enteritidis were isolated and tested by PCR for the presence of genes associated with pathogenicity islands of E. coli: hlyA, hlyB, sfaG, and sfaA.


RESULTS
It was determined that DNA fragments homologous to pathogenicity islands of E. coli were present in 87 (96.7%) of S. enteritidis clinical isolates. Disease caused by Salmonella strains which possess only sfaG was mostly mild--7 (33.3%), whereas strains which had sfaG with fragments of hlyA and/or hlyB caused severe disease--7 (50%). sfaA fragments were found mostly in combination with other genes. In such cases the disease was mostly severe--6 (42.8%).


CONCLUSIONS
Correlation between presence of E. coli pathogenicity islands in Salmonella spp., their antibiotic resistance and severity of infection was established.
A R
AR
Mavziutov
R T
RT
Murzabaeva
R G
RG
Nazmutdinova
I A
IA
Mirsaiapova
D018830:Adhesins, Escherichia coli / Q000032:analysis; D000328:Adult; D001426:Bacterial Proteins / Q000032:analysis; D002352:Carrier Proteins / Q000032:analysis; D004352:Drug Resistance, Microbial / Q000235:genetics; D029968:Escherichia coli Proteins / Q000032:analysis / Q000235:genetics; D005260:Female; D036781:Fimbriae Proteins / Q000032:analysis; D044404:Genomic Islands / Q000235:genetics; D006460:Hemolysin Proteins / Q000032:analysis; D006801:Humans; D008297:Male; D012480:Salmonella Infections / Q000382:microbiology* / Q000503:physiopathology*; D012477:Salmonella enteritidis / Q000235:genetics* / Q000472:pathogenicity*; D014774:Virulence / Q000235:genetics
D018830:Adhesins, Escherichia coli; D001426:Bacterial Proteins; D002352:Carrier Proteins; D029968:Escherichia coli Proteins; D006460:Hemolysin Proteins; C036507:Hlya protein, E coli; C050837:Hlyb protein, Bacteria; C087444:sfaA protein, E coli; D036781:Fimbriae Proteins