Quantitative detection of glyphosate by simultaneous analysis of UV spectroscopy and fluorescence using DNA-labeled gold nanoparticles.
A sandwich-type immunosensor composed of antigen-double target/probe DNA-coated gold nanoparticles (NPs) was developed for the measurement of fluorescence intensity and quantitative analysis of single-stranded DNA based on the concentration of free glyphosate. The reaction between the antigen-double DNA-gold NPs and immobilized antibody on the substrate was carried out for 2 h. The results of the antigen-antibody reaction were measured on the basis of the fluorescence intensity obtained from comparison with the free antigens at concentrations of 0.01-100 μg mL(-1) for the detection of immobilized antigen-double DNA-gold NPs. For the quantitative analysis based on the concentration of glyphosate(0.01-100 μg mL(-1)), the immunosensor response also revealed the same detection range of glyphosate using DNA detection.
Department of Chemical and Biological Engineering, Korea University, 5 Ga, Anam-Dong, Sungbuk-Gu, Seoul 136-701, Korea.
Hee Uk
HU
Lee
Hyun Yong
HY
Shin
Jin Young
JY
Lee
Yoon Seok
YS
Song
ChulHwan
C
Park
Seung Wook
SW
Kim
D015342:DNA Probes / Q000737:chemistry*; D005453:Fluorescence; D005998:Glycine / Q000031:analogs & derivatives* / Q000032:analysis; D006046:Gold / Q000737:chemistry*; D006540:Herbicides / Q000032:analysis*; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods*; D056951:Photoelectron Spectroscopy; D000097797:Glyphosate
D015342:DNA Probes; D005998:Glycine; D006046:Gold; D006540:Herbicides; D000097797:Glyphosate