[Determination of berberine chloride in mouse plasma after intragastric administration of Lianxiang microemulsion].
OBJECTIVE
To determine berberine chloride in mouse plasma for the investigation of the bioavailability of Lianxiang microemulsion in mouse.


METHODS
After single dose ig administration,the plasma samples were taken at different time,respectively, for the determination of berberine chloride by HPLC; And the relative bioavailability of Lianxiang microemulsion vs Lianxiang emulsion (reference formulation) was calculated.


RESULTS
Berberine chloride was separated well from endogenous foreign substances. The calibration curve of berberine chloride was liner over the range of 10.4-156 microg/L (r = 0.9991) and the method recovery was within 89.6%-94.7%. The intraday RSD or the inter-day RSD < or = 12.9%. After single dose,the AUC0-->24h of the microemulsion and the emulsion were (688.3 +/- 123.7) and (371.4 +/- 68.4) microg x h/L,respectively; And the relative bioavailability of the microemulsion vs the emulsion was (193.1 +/- 63.2)%.


CONCLUSIONS
The validated HPLC method is suitable for the determination of berberine chloride in mouse plasma; And microemulsification can clearly promote the bioavailability in mouse of berberine chloride in Lianxiang prescription.
Guangzhou University of Traditional Chinese Medicine,Guangzhou 510405, China. vip.luwbl@gzhtcm.edu.cn
Wen-Biao
WB
Lu
Wei-Wen
WW
Chen
Ru-Liu
RL
Li
D000284:Administration, Oral; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage / Q000493:pharmacokinetics*; D001599:Berberine / Q000097:blood* / Q000493:pharmacokinetics; D001682:Biological Availability; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D039323:Coptis / Q000737:chemistry*; D004339:Drug Compounding / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000493:pharmacokinetics*; D004655:Emulsions; D005260:Female; D008297:Male; D051379:Mice; D010316:Particle Size; D011897:Random Allocation; D027842:Syzygium / Q000737:chemistry*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D004365:Drugs, Chinese Herbal; D004655:Emulsions; D001599:Berberine