Role of glucocorticoid receptor in the inhibitory effect of medroxyprogesterone acetate on the estrogen-induced endothelial nitric oxide synthase phosphorylation in human umbilical vein endothelial cells.
We aimed to clarify which steroid receptor is involved in the inhibitory effect of medroxyprogesterone acetate (MPA) on estrogen-induced endothelial nitric oxide synthase (eNOS) phosphorylation in human umbilical vein endothelial cells (HUVEC). The progesterone/glucocorticoid receptor (GR) antagonist RU-486 and introduction of GR siRNA caused attenuation of the inhibitory effect of MPA on the estrogen-induced eNOS phosphorylation and eNOS activity in HUVEC.
Department of Obstetrics and Gynecology, Dokkyo University School of Medicine, Mibu, Shimotsuga, Tochigi, Japan.
Akira
A
Oishi
Kazuhiro
K
Takahashi
Masahide
M
Ohmichi
Yoshiko
Y
Mochizuki
Noriyuki
N
Inaba
Hirohisa
H
Kurachi
D050197:Atherosclerosis / Q000453:epidemiology / Q000378:metabolism / Q000517:prevention & control; D002478:Cells, Cultured; D003271:Contraceptive Agents, Female / Q000494:pharmacology; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D004967:Estrogens / Q000378:metabolism*; D006727:Hormone Antagonists / Q000494:pharmacology; D006801:Humans; D017258:Medroxyprogesterone Acetate / Q000494:pharmacology*; D015735:Mifepristone / Q000494:pharmacology; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism*; D010766:Phosphorylation / Q000187:drug effects; D034741:RNA, Small Interfering / Q000494:pharmacology; D011965:Receptors, Glucocorticoid / Q000235:genetics / Q000378:metabolism*; D012307:Risk Factors; D014471:Umbilical Veins / Q000166:cytology
D003271:Contraceptive Agents, Female; D004967:Estrogens; D006727:Hormone Antagonists; D034741:RNA, Small Interfering; D011965:Receptors, Glucocorticoid; D015735:Mifepristone; D017258:Medroxyprogesterone Acetate; C496314:NOS3 protein, human; D052250:Nitric Oxide Synthase Type III