The proteome analysis of oleaginous yeast Lipomyces starkeyi.
Oleaginous yeast Lipomyces starkeyi, a species in the Saccharomycetales order, has the capability to accumulate over 70% of its cell biomass as lipid under defined culture conditions. In this study, analysis of L. starkeyi AS 2.1560 proteome samples from different culture stages during a typical lipid production process was performed using an online multidimensional μRPLC/MS/MS method. Data searching against the proteome database of the yeast Saccharomyces cerevisiae led to the identification of 289 protein hits. Further comparative and semi-quantitative analysis under more stringent criteria revealed 81 proteins with significant expression-level changes. Among them, 52 proteins were upregulated and 29 proteins were downregulated. Gene ontology annotation indicated that global responses occurred when cells were exposed to the nitrogen deficiency environment for lipid production. Protein hits were annotated and largely concerned metabolic processes for alternative nitrogen sources usage or lipid accumulation. Many of the downregulated proteins were related to glycolysis, whereas the majority of the upregulated proteins were involved in proteolysis and peptidolysis, carbohydrate metabolism and lipid metabolism. Insights were provided in terms of cellular responses to nutrient availability as well as the basic biochemistry of lipid accumulation. This work presented potentially valuable information for understanding the biochemical events related to microbial oleaginity and rational engineering of oleaginous yeasts.
CAS Key Lab of Separation for Analytical Chemistry, Dalian Institute of Chemical Physics, CAS, Dalian, China Graduate School of the Chinese Academy of Sciences, Beijing, China Division of Biotechnology, Dalian Institute of Chemical Physics, CAS, Dalian, China.
Hongwei
H
Liu
Xin
X
Zhao
Fangjun
F
Wang
Xinning
X
Jiang
Sufang
S
Zhang
Mingliang
M
Ye
Zongbao K
ZK
Zhao
Hanfa
H
Zou
D056148:Chromatography, Reverse-Phase; D005656:Fungal Proteins / Q000032:analysis*; D055160:Lipomyces / Q000737:chemistry*; D020543:Proteome / Q000032:analysis*; D053719:Tandem Mass Spectrometry
D005656:Fungal Proteins; D020543:Proteome