Cell-based screening using high-throughput flow cytometry.
This review describes the use of high-throughput flow cytometry for performing multiplexed cell-based and bead-based screens. With the many advances in cell-based analysis and screening, flow cytometry has historically been underutilized as a screening tool largely due to the limitations in handling large numbers of samples. However, there has been a resurgence in the use of flow cytometry due to a combination of innovations around instrumentation and a growing need for cell-based and bead-based applications. The HTFC™ Screening System (IntelliCyt Corporation, Albuquerque, NM) is a novel flow cytometry-based screening platform that incorporates a fast sample-loading technology, HyperCyt®, with a two-laser, six-parameter flow cytometer and powerful data analysis capabilities. The system is capable of running multiplexed screening assays at speeds of up to 40 wells per minute, enabling the processing of a 96- and 384-well plates in as little as 3 and 12 min, respectively. Embedded in the system is HyperView®, a data analysis software package that allows rapid identification of hits from multiplexed high-throughput flow cytometry screening campaigns. In addition, the software is incorporated into a server-based data management platform that enables seamless data accessibility and collaboration across multiple sites. High-throughput flow cytometry using the HyperCyt technology has been applied to numerous assay areas and screening campaigns, including efflux transporters, whole cell and receptor binding assays, functional G-protein-coupled receptor screening, in vitro toxicology, and antibody screening.
IntelliCyt Corporation, 317 Commercial St. NE, Albuquerque, NM 87102, USA. cblack@intellicyt.com
Christopher B
CB
Black
Thomas D
TD
Duensing
Linda S
LS
Trinkle
R Terry
RT
Dunlay
D001681:Biological Assay / Q000295:instrumentation*; D002468:Cell Physiological Phenomena / Q000187:drug effects*; D004353:Drug Evaluation, Preclinical / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005434:Flow Cytometry / Q000295:instrumentation*; D017022:Flow Injection Analysis / Q000295:instrumentation*; D057166:High-Throughput Screening Assays / Q000295:instrumentation*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*