Ex vivo assessment of protective effects of carvacrol against DNA lesions induced in primary rat cells by visible light excited methylene blue (VL+MB).
Carvacrol belongs to frequently occurring phenolic components of essential oils (EOs) and it is present in many kinds of plants. Biological effect of this phenol derivative on human beings is however not sufficiently known. The present study was undertaken to evaluate the level of VL+MB-induced oxidative DNA lesions in hepatocytes and testicular cells (freshly isolated from control or carvacrol-watered rats) by the modified single cell gel electrophoresis (SCGE). The results showed that carvacrol significantly reduced the level of VL+MB-induced oxidized bases (EndoIII- and Fpg-sensitive sites) only in hepatocytes but not in testicular cells. Chromosomal aberration assay of primary hepatocytes, isolated from control or carvacrol-watered rats did not testify any genotoxic activity of carvacrol. We suggest that in vivo applied synthetic carvacrol, whose antioxidative activity was confirmed by DPPH assay, exhibits primarily a strong hepatoprotective activity against oxidative damage to DNA.
Cancer Resaerch Institute, Slovak Academy of Sciences, 833 91 Bratislava, Slovakia. darina.slamenova@savka.sk
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Slamenova
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Horvathova
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Chalupa
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Wsolova
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Navarova
D000818:Animals; D002869:Chromosome Aberrations; D000081007:Cymenes; D004249:DNA Damage; D022781:Hepatocytes / Q000187:drug effects; D008027:Light; D008297:Male; D008751:Methylene Blue / Q000633:toxicity*; D039821:Monoterpenes / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D026082:Singlet Oxygen / Q000633:toxicity; D013737:Testis / Q000187:drug effects
D000081007:Cymenes; D039821:Monoterpenes; D026082:Singlet Oxygen; C073316:carvacrol; D008751:Methylene Blue