Perfusion-based microfluidic device for three-dimensional dynamic primary human hepatocyte cell culture in the absence of biological or synthetic matrices or coagulants.
We describe a perfusion-based microfluidic device for three-dimensional (3D) dynamic primary human hepatocyte cell culture. The microfluidic device was used to promote and maintain 3D tissue-like cellular morphology and cell-specific functionality of primary human hepatocytes by restoring membrane polarity and hepatocyte transport function in vitro without the addition of biological or synthetic matrices or coagulants. A unique feature of our dynamic cell culture device is the creation of a microenvironment, without the addition of biological or synthetic matrices or coagulants, that promotes the 3D organization of hepatocytes into cord-like structures that exhibit functional membrane polarity as evidenced by the expression of gap junctions and the formation of an extended, functionally active, bile canalicular network.
Science & Technology, Corning Incorporated, Corning, New York 14831-0001, USA.
Vasiliy N
VN
Goral
Yi-Cheng
YC
Hsieh
Odessa N
ON
Petzold
Jeffery S
JS
Clark
Po Ki
PK
Yuen
Ronald A
RA
Faris
D000255:Adenosine Triphosphate / Q000737:chemistry; D001648:Bile Canaliculi / Q000166:cytology; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods*; D002478:Cells, Cultured; D003029:Coagulants / Q000737:chemistry; D004867:Equipment Design; D017629:Gap Junctions; D022781:Hepatocytes / Q000166:cytology*; D006801:Humans; D021621:Imaging, Three-Dimensional / Q000379:methods*; D046210:Microfluidic Analytical Techniques; D008856:Microscopy, Fluorescence / Q000379:methods; D008954:Models, Biological; D000089762:Multidrug Resistance-Associated Protein 2; D027425:ATP-Binding Cassette, Sub-Family C Proteins / Q000378:metabolism; D010477:Perfusion
D000255:Adenosine Triphosphate; D003029:Coagulants; D000089762:Multidrug Resistance-Associated Protein 2; D027425:ATP-Binding Cassette, Sub-Family C Proteins