Myeloperoxidase activity is increased in gingival crevicular fluid and whole saliva after fixed orthodontic appliance activation.
BACKGROUND
Orthodontic tooth movement uses mechanical forces that result in inflammation in the first days. Myeloperoxidase (MPO) is an enzyme found in polymorphonuclear neutrophil (PMN) granules, and it is used to estimate the number of PMN granules in tissues. So far, MPO has not been used to study the inflammatory alterations after the application of orthodontic tooth movement forces. The aim of this study was to determine MPO activity in the gingival crevicular fluid (GCF) and saliva (whole stimulated saliva) of orthodontic patients at different time points after fixed appliance activation.


METHODS
MPO was determined in the GCF and collected by means of periopaper from the saliva of 14 patients with orthodontic fixed appliances. GCF and saliva samples were collected at baseline, 2 hours, and 7 and 14 days after application of the orthodontic force.


RESULTS
Mean MPO activity was increased in both the GCF and saliva of orthodontic patients at 2 hours after appliance activation (P <0.02 for all comparisons). At 2 hours, PMN infiltration into the periodontal ligament from the orthodontic force probably results in the increased MPO level observed at this time point.


CONCLUSIONS
MPO might be a good marker to assess inflammation in orthodontic movement; it deserves further studies in orthodontic therapy.
Department of Morphology, Stomatology and Physiology, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil. amarcaccini@hotmail.com
Andrea M
AM
Marcaccini
Patricia A F
PA
Amato
Fernanda V
FV
Leão
Raquel F
RF
Gerlach
Jose T L
JT
Ferreira
D002648:Child; D005260:Female; D005500:Follow-Up Studies; D005883:Gingival Crevicular Fluid / Q000201:enzymology*; D006801:Humans; D008297:Male; D020556:Neutrophil Infiltration / Q000502:physiology; D009504:Neutrophils / Q000201:enzymology; D009969:Orthodontic Wires; D010513:Periodontal Ligament / Q000473:pathology; D009195:Peroxidase / Q000032:analysis*; D012463:Saliva / Q000201:enzymology*; D013314:Stress, Mechanical; D014087:Tooth Movement Techniques / Q000295:instrumentation
D009195:Peroxidase