Postpartum toll-like receptors and β-defensin 5 mRNA levels in the endometrium of Holstein cows.
Toll-like receptors (TLRs) and β-defensins are important components of the innate immune system. This study aimed to evaluate endometrial mRNA levels of TLRs (1/6, 2, 4, and 5) and β-defensin 5 in Holstein cows by quantitative real time RT-PCR. Uterine biopsies were performed from 6 to 12 h after parturition, and cows were divided into two groups: (i) cows with placental retention and clinical signs of uterine infection until 45 days postpartum (n=10) or (ii) cows with normal puerperium (n=10). All cows had detectable levels of TLRs and β-defensin 5 mRNAs, but these levels did not differ between groups (P>0.05). Levels of TLR4 mRNA had a positive and significant correlation with the time required for uterine involution in both groups.
Departamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, 31270-901 Belo Horizonte, MG, Brazil.
Telma da Mata
Tda M
Martins
Tatiane Alves
TA
da Paixão
Érica Azevedo
ÉA
Costa
Alexandre de Carvalho
Ade C
Pires
Renato Lima
RL
Santos
Álan Maia
ÁM
Borges
D000818:Animals; D002417:Cattle; D004717:Endometrium / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology*; D049590:Postpartum Period / Q000502:physiology*; D011247:Pregnancy; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D051193:Toll-Like Receptors / Q000378:metabolism*; D023083:beta-Defensins / Q000235:genetics / Q000378:metabolism*
D012333:RNA, Messenger; D051193:Toll-Like Receptors; D023083:beta-Defensins