Association of lymphocyte crossmatch and the outcome of intestinal transplantation in swine.
BACKGROUND
The effect of preformed antidonor antibodies have been demonstrated in various types of solid organ transplantation. However, the significance of anti-donor antibodies in intestinal transplantation remains unclear. The aim of this study is to evaluate the impact that the extent of T cell crossmatch has on the outcome of swine intestinal transplantation.


METHODS
All studies were performed on outbred domestic male pigs weighing from 15 to 20 kg. Intestinal transplantation was performed orthotopically with an exchange of grafts between white and black pigs. FK506 was administered intravenously (0.1 mg/kg per day, POD 0-7) for immunosuppression. A lymphocyte crossmatch test was performed using the direct CDC crossmatch. The results were considered positive when more than 10% of the donor lymphocytes were killed by the recipient's serum. In addition, 0-10, 11-20, 21-30, 31-80 and 81-100% of the killed lymphocytes were classified as grade 1, 2, 4, 6 and 8, respectively.


RESULTS
A total of 34 intestinal transplantations were performed. All but one case had positive donor specific T cell crossmatches. The number of grade 2, 4, 6 and 8 cases was 11, 14, 6 and 2, respectively. Although there was a tendency towards a decreased survival according to the grade, the survival rate was not statistically different among each different grade. Moreover, the rates of acute cellular rejection and vascular complications were not significantly different among the four grades.


CONCLUSIONS
These results suggest that the extent of positive T cell crossmatch is not associated with the outcome of swine intestinal transplantation.
Department of Pediatric Surgery, Reproductive and Developmental Medicine, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, Japan. makoto-h@pedsurg.med.kyushu-u.ac.jp
Makoto
M
Hayashida
Toshiharu
T
Matsuura
Isamu
I
Saeki
Yusuke
Y
Yanagi
Koichiro
K
Yoshimaru
Yuko
Y
Nishimoto
Yukiko
Y
Takahashi
Keiko
K
Fujita
Narito
N
Takada
Shohei
S
Taguchi
Toru
T
Uesugi
Ryuichiro
R
Hirose
Masatoshi
M
Nakamura
Makoto
M
Nakao
Tomoaki
T
Taguchi
D000818:Animals; D006084:Graft Rejection / Q000276:immunology; D006085:Graft Survival / Q000276:immunology; D006650:Histocompatibility Testing; D007165:Immunosuppression Therapy / Q000379:methods; D007166:Immunosuppressive Agents / Q000494:pharmacology; D007422:Intestines / Q000637:transplantation*; D008297:Male; D034421:Sus scrofa; D013601:T-Lymphocytes / Q000276:immunology*; D016559:Tacrolimus / Q000494:pharmacology
D007166:Immunosuppressive Agents; D016559:Tacrolimus