Biaryl modification of the 5'-terminus of one strand of a microRNA duplex induces strand specificity.
MicroRNAs (miRNAs) are single-stranded non-coding RNAs composed of 20-23 nucleotides. They are initially transcribed in the nucleus as pri-miRNAs. After processing, one strand from the miRNA duplex (miR-5p/miR-3p duplex) is loaded onto the RNA-induced silencing complex (RISC) to produce a functional, mature miRNA that inhibits the expression of multiple target genes. In the case of some miRNAs, both strands can be equally incorporated into the RISC as single strands, and both strands can function as mature miRNAs. Thus, a technique for selective expression of miR-5p and miR-3p strands is required to identify distinct targets of miRNAs. In this Letter, we report the synthesis and properties of miRNA duplexes carrying biaryl units at the 5'-terminus of one strand. We found that incorporation of biaryl units at the 5'-terminus of one strand of miRNA duplexes induced strand specificity in these duplexes. Further, we succeeded in identifying endogenous mRNA targets for each strand of the duplex by using the biaryl-modified miRNA duplexes.
Department of Biomolecular Science, Faculty of Engineering, Gifu University, Gifu, Japan.
Aya
A
Ogata
Chihiro
C
Furukawa
Kouhei
K
Sakurai
Hideo
H
Iba
Yukio
Y
Kitade
Yoshihito
Y
Ueno
D001483:Base Sequence; D035683:MicroRNAs / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D009281:Naphthalenes / Q000737:chemistry*; D034622:RNA Interference; D034743:RNA-Induced Silencing Complex / Q000737:chemistry / Q000378:metabolism
D035683:MicroRNAs; D009281:Naphthalenes; D034743:RNA-Induced Silencing Complex; C031721:naphthalene