Cecal ligation and puncture.
The cecum contains a high concentration of microbes, which are a combination of Gram-negative and Gram-positive flora. These bacteria range from anaerobic to facultative aerobic to aerobic organisms. In the procedure described in this unit, the ligation of the cecum produces a source of ischemic tissue as well as polymicrobial infection. This combination of ischemic/necrotic tissue and microbial infection distinguishes this multifactorial model from a number of other bacterial sepsis models, including but not limited to: bacteremia secondary to intravenous or intraperitoneal administration; fecal administration or intraperitoneal administration of fecal or bacterial plugs; colonic stents; and bacterial abscess formation.
University of Florida College of Medicine, Gainesville, Florida, USA.
Alex G
AG
Cuenca
Matthew J
MJ
Delano
Kindra M
KM
Kelly-Scumpia
Lyle L
LL
Moldawer
Philip A
PA
Efron
D000818:Animals; D002432:Cecum / Q000293:injuries* / Q000382:microbiology*; D004195:Disease Models, Animal; D006801:Humans; D007511:Ischemia / Q000209:etiology / Q000382:microbiology; D008026:Ligation / Q000379:methods; D051379:Mice; D010538:Peritonitis / Q000209:etiology / Q000382:microbiology; D011677:Punctures / Q000379:methods; D018805:Sepsis / Q000209:etiology* / Q000382:microbiology*; D014947:Wounds and Injuries / Q000209:etiology / Q000382:microbiology