Evaluation of TLR4 expression and chosen parameters of oxidative-antioxidative balance in young children with food allergy.
The authors evaluated mRNA TLR4 expression on neutrophils and the chosen parameters of oxidative-antioxidative balance in blood of 35 children with food allergy (17 of them with IgE-dependent allergy and 18 with IgE-independent allergy) and 15 healthy children without any allergy. The age of these children ranged from 1 to 36 months. Children with food allergy in comparison with healthy children were found to have lower mRNA TLR4 expression, higher average value of chemiluminescence (CL) and its increase after stimulation by fMLP, PMA and OZ as well as lower TAS values. Disturbances of oxidative-antioxidative balance were found in children with food allergy. We suggest that natural immunity is involved in the development of food allergy mechanisms. Moreover, chemiluminescence can be used as an additional diagnostic test.
Second Department of Pediatrics and Allergology, Polish Mother's Memorial Hospital - Research Institute, Łódż, Poland. bkamer@wp.pl
Barbara
B
Kamer
Karolina
K
Kulig
Przemysław
P
Lewkowicz
Anna
A
Kamer-Bartosińska
Henryk
H
Tchórzewski
D000975:Antioxidants / Q000378:metabolism*; D016022:Case-Control Studies; D002675:Child, Preschool; D005512:Food Hypersensitivity / Q000175:diagnosis / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000276:immunology / Q000378:metabolism; D007113:Immunity, Innate; D007223:Infant; D008163:Luminescent Measurements; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000276:immunology / Q000378:metabolism; D018384:Oxidative Stress; D012333:RNA, Messenger / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D051197:Toll-Like Receptor 4 / Q000378:metabolism*; D015054:Zymosan / Q000494:pharmacology
D000975:Antioxidants; D012333:RNA, Messenger; C495345:TLR4 protein, human; D051197:Toll-Like Receptor 4; D009240:N-Formylmethionine Leucyl-Phenylalanine; D015054:Zymosan; D013755:Tetradecanoylphorbol Acetate