The potential of magnetic nanocluster and dual-functional protein-based strategy for noninvasive detection of HBV surface antibodies.
Magnetic nanoclusters (MNCs) were synthesized in a one-pot process, carboxylic MNCs and dual-functional protein were prepared and used to capture hepatitis B virus surface antibodies (anti-HBs) in simulated diseased oral mucosal transudate (OMT) samples. The specific substrate of dual-functional protein, dual-labeled double-chained DNA molecules, based on Fluorescence Resonance Energy Transfer (FRET), was used to amplify the detection signal and the detection limit of 0.1 ng mL(-1) of anti-HBs monoclonal antibodies was achieved. Combination MNCs with dual-functional protein enables the noninvasive detection of hepatitis B virus (HBV) surface antibodies in OMT samples, showing promise as a diagnostic tool for the OMT diagnosis of infectious diseases with sensitive, specific and facile capabilities.
Department of Bio-nano-Science and Engineering, Key Laboratory for Thin Film and Microfabrication of Ministry of Education, Institute of Micro-Nano Science and Technology, Shanghai Jiao Tong University, PR China.
Hengyao
H
Hu
Hao
H
Yang
Ding
D
Li
Kan
K
Wang
Jing
J
Ruan
Xueqing
X
Zhang
Jun
J
Chen
Chenchen
C
Bao
Jiajia
J
Ji
Donglu
D
Shi
Daxiang
D
Cui
D015374:Biosensing Techniques / Q000379:methods*; D015342:DNA Probes / Q000737:chemistry / Q000378:metabolism; D006510:Hepatitis B Antibodies / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D006514:Hepatitis B Surface Antigens / Q000737:chemistry / Q000276:immunology; D006515:Hepatitis B virus / Q000302:isolation & purification*; D008280:Magnetics; D049329:Nanostructures / Q000737:chemistry*; D011506:Proteins / Q000378:metabolism*
D015342:DNA Probes; D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D011506:Proteins