Inhibitory effect of a bioactivity-guided fraction from Rheum undulatum on the acid production of Streptococcus mutans biofilms at sub-MIC levels.
Rheum undulatum root has been used traditionally in Korea for the treatment of dental diseases. The purpose of this study was to separate a fraction from R. undulatum showing anti-acid production activity against Streptococcus mutans biofilms and identify the main components in that fraction. Methanol extract of R. undulatum root and its fractions were prepared. To select a fraction exhibiting anti-acid production activity, suspension glycolytic pH-drop assay was performed. Among the fractions tested, dichloromethane fraction exhibited the strongest activity in a dose-dependent manner. To examine the effect of the selected fraction on the anti-acid production of S. mutans biofilms, 74 h old S. mutans biofilms were used. The selected fraction reduced the initial rate of acid production of S. mutans biofilms at sub-minimum inhibitory concentration (MIC) levels. HPLC qualitative analysis of the selected fraction indicated that the presence of anthraquinone derivatives, such as aloe-emodin, emodin, chrysophanol and physcion, as main components.
Department of Preventive Dentistry, School of Dentistry, Chonbuk National University, Jeonju, Republic of Korea.
Jeong-Eun
JE
Kim
Hye-Jin
HJ
Kim
Santosh
S
Pandit
Kee-Wan
KW
Chang
Jae-Gyu
JG
Jeon
D000143:Acids / Q000378:metabolism*; D000880:Anthraquinones / Q000494:pharmacology* / Q000627:therapeutic use; D018441:Biofilms / Q000187:drug effects*; D002851:Chromatography, High Pressure Liquid; D003731:Dental Caries / Q000517:prevention & control*; D008826:Microbial Sensitivity Tests; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D018517:Plant Roots; D012250:Rheum / Q000737:chemistry*; D013295:Streptococcus mutans / Q000187:drug effects* / Q000378:metabolism
D000143:Acids; D000880:Anthraquinones; D010936:Plant Extracts