Decreased antioxidase activities and oxidative stress in the spleen of chickens fed on high-fluorine diets.
Three hundred one-day-old avian broilers were divided into four equal groups of 75 animals that were fed for 42 days as follows: a control diet containing 23 mg fluorine (F)/kg and three high F diets containing 400, 800, and 1200 mg F/kg, respectively, for high F groups I, II, and III. The superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities were greatly decreased, while the malondialdehyde (MDA) contents were markedly increased in high F groups II and III. At the same time, mitochondrial injury and expanded endocytoplasmic reticulum were obviously observed in high F groups II and III, and the fluoride contents both in spleen and serum were significantly increased in the three high F groups when compared with those of control group. The results showed that excess dietary F in the range of 800-1200 mg/kg caused obvious oxidative stress, which provided a possible pathway for the apoptosis of splenocytes in chickens.
Department of Veterinary Medicine, Sichuan Agricultural University, Ya'an, Sichuan, China.
Tao
T
Chen
Hengmin
H
Cui
Yun
Y
Cui
Caimin
C
Bai
Tao
T
Gong
D000704:Analysis of Variance; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D017209:Apoptosis / Q000187:drug effects; D002645:Chickens / Q000254:growth & development; D004032:Diet; D004305:Dose-Response Relationship, Drug; D004721:Endoplasmic Reticulum / Q000187:drug effects; D005459:Fluorides / Q000097:blood / Q000493:pharmacokinetics / Q000633:toxicity*; D005979:Glutathione Peroxidase / Q000097:blood / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D008928:Mitochondria / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D013154:Spleen / Q000187:drug effects* / Q000201:enzymology / Q000254:growth & development / Q000473:pathology; D013482:Superoxide Dismutase / Q000097:blood / Q000378:metabolism; D013997:Time Factors
D000975:Antioxidants; D008315:Malondialdehyde; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005459:Fluorides