Photophysical properties of Zn-substituted cytochrome c investigated by single-molecule and ensemble-averaged spectroscopy.
The study of the structural reorganization and photophysical properties of Zn-Cytc using the single-molecule and ensemble-averaged spectroscopy shows that the photoblinking behaviors of single-Zn-Cytc depend on the folded and unfolded structures, whereas the fluorescence dynamics of Zn-Cytc observed in the bulk phase are hardly affected by the conformational change of a protein.
The Institute of Scientific and Industrial Research (SANKEN), Osaka University, Mihogaoka 8-1, Ibaraki, Osaka 567-0047, Japan.
Jungkweon
J
Choi
Takashi
T
Tachikawa
Youngmin
Y
Kim
Mamoru
M
Fujitsuka
Hyotcherl
H
Ihee
Tetsuro
T
Majima
D045304:Cytochromes c / Q000737:chemistry*; D005453:Fluorescence; D055668:Photochemical Processes / Q000528:radiation effects*; D011487:Protein Conformation; D017510:Protein Folding; D013057:Spectrum Analysis / Q000379:methods*; D015032:Zinc / Q000737:chemistry*
D045304:Cytochromes c; D015032:Zinc