Nonnegative matrix factorization: a blind spectra separation method for in vivo fluorescent optical imaging.
Fluorescence imaging in diffusive media is an emerging imaging modality for medical applications that uses injected fluorescent markers that bind to specific targets, e.g., carcinoma. The region of interest is illuminated with near-IR light and the emitted back fluorescence is analyzed to localize the fluorescence sources. To investigate a thick medium, as the fluorescence signal decreases with the light travel distance, any disturbing signal, such as biological tissues intrinsic fluorescence (called autofluorescence) is a limiting factor. Several specific markers may also be simultaneously injected to bind to different molecules, and one may want to isolate each specific fluorescent signal from the others. To remove the unwanted fluorescence contributions or separate different specific markers, a spectroscopic approach is explored. The nonnegative matrix factorization (NMF) is the blind positive source separation method we chose. We run an original regularized NMF algorithm we developed on experimental data, and successfully obtain separated in vivo fluorescence spectra.
CEA-LETI, Minatec, 17 rue des Martyrs, Grenoble Cedex 9, 38054, France and GIPSA-Lab/DIS, CNRS, UMR 5216, BP Saint Martin d'Hères Cedex, France. anne-sophie.montcuquet@cea.fr
Anne-Sophie
AS
Montcuquet
Lionel
L
Hervé
Fabrice
F
Navarro
Jean-Marc
JM
Dinten
Jérôme I
JI
Mars
D000465:Algorithms; D000818:Animals; D001940:Breast / Q000033:anatomy & histology; D001943:Breast Neoplasms / Q000175:diagnosis; D003952:Diagnostic Imaging / Q000379:methods* / Q000706:statistics & numerical data; D005260:Female; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000008:administration & dosage; D006801:Humans; D051379:Mice; D055620:Optical Phenomena; D019047:Phantoms, Imaging; D013050:Spectrometry, Fluorescence; D019265:Spectroscopy, Near-Infrared; D013057:Spectrum Analysis
D005456:Fluorescent Dyes