Hypogelsolinemia, a disorder of the extracellular actin scavenger system, in patients with multiple sclerosis.
BACKGROUND
Extracellular gelsolin (GSN) and GC-globulin/Vitamin D-binding protein (DBP) appear to play an important role in clearing the actin from extracellular fluids and in modulating cellular responses to anionic bioactive lipids. In this study we hypothesized that cellular actin release and/or increase in bioactive lipids associated with multiple sclerosis (MS) development will translate into alteration of the actin scavenger system protein concentrations in blood and cerebrospinal fluid (CSF) of patients with MS.


METHODS
We measured GSN and DBP concentrations in blood and CSF obtained from patients diagnosed with MS (n = 56) in comparison to a control group (n = 20) that includes patients diagnosed with conditions such as idiopathic cephalgia (n = 11), idiopathic (Bell's) facial nerve palsy (n = 7) and ischialgia due to discopathy (n = 2). GSN and DBP levels were measured by Western blot and ELISA, respectively.


RESULTS
We found that the GSN concentration in the blood of the MS group (115 ± 78 μg/ml) was significantly lower (p < 0.001) compared to the control group (244 ± 96 μg/ml). In contrast, there was no statistically significant difference between blood DBP concentrations in patients with MS (310 ± 68 μg/ml) and the control group (314 ± 82 μg/ml). GSN and DBP concentrations in CSF also did not significantly differ between those two groups.


CONCLUSIONS
The decrease of GSN concentration in blood and CSF of MS subjects suggests that this protein may be involved in chronic inflammation associated with neurodegeneration. Additionally, the results presented here suggest the possible utility of GSN evaluation for diagnostic purposes. Reversing plasma GSN deficiency might represent a new strategy in MS treatment.
Department of Neurology, Medical University of Białystok, 15-230 Białystok, Poland.
Alina
A
Kułakowska
Nicholas J
NJ
Ciccarelli
Qi
Q
Wen
Barbara
B
Mroczko
Wiesław
W
Drozdowski
Maciej
M
Szmitkowski
Paul A
PA
Janmey
Robert
R
Bucki
D000199:Actins / Q000378:metabolism*; D000328:Adult; D004797:Enzyme-Linked Immunosorbent Assay; D045604:Extracellular Fluid / Q000737:chemistry / Q000378:metabolism*; D005260:Female; D018260:Gelsolin / Q000032:analysis / Q000378:metabolism*; D006801:Humans; D015151:Immunoblotting; D008297:Male; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000378:metabolism*; D014809:Vitamin D-Binding Protein / Q000032:analysis / Q000378:metabolism*
D000199:Actins; D018260:Gelsolin; D014809:Vitamin D-Binding Protein