Three-dimensional optical imaging of microvascular networks within intact lymph node in vivo.
Sentinel lymph nodes (SLNs) are the first lymph nodes to drain wastes originated from cancerous tissue. There is a need for an in vivo imaging method that can image the intact SLN to further our understanding of its normal as well as abnormal functions. We report the use of ultrahigh sensitive optical microangiography (UHS-OMAG) to image functional microvascular and lymphatic vessel networks that innervate the intact lymph node in mice in vivo. The promising results show a potential role of UHS-OMAG in the future understanding and diagnosis of the SLN involvement in cancer development.
Oregon Health & Science University, Department of Biomedical Engineering 3303 SW Bond Avenue, Portland, Oregon 97239, USA.
Yeongri
Y
Jung
Zhongwei
Z
Zhi
Ruikang K
RK
Wang
D000818:Animals; D001943:Breast Neoplasms / Q000098:blood supply / Q000556:secondary; D005260:Female; D006801:Humans; D021621:Imaging, Three-Dimensional; D008198:Lymph Nodes / Q000098:blood supply*; D042601:Lymphatic Vessels / Q000033:anatomy & histology; D051379:Mice; D008810:Mice, Inbred C57BL; D055806:Microvessels / Q000033:anatomy & histology*; D021701:Sentinel Lymph Node Biopsy; D041623:Tomography, Optical Coherence / Q000379:methods*