Prevention of NKT cell activation accelerates cutaneous wound closure and alters local inflammatory signals.
We previously reported that in the absence of NKT cells, wound closure was accelerated in a murine excisional punch wound model. Here, we explored whether purposefully inhibiting NKT cell activation had similar effects on wound closure and the dermal inflammatory response to injury. We found that prevention of NKT cell activation accelerated wound closure in a dose-responsive manner. If anti-CD1d was administered before wounding, NKT cell infiltration into cutaneous wounds was diminished without quantitative changes in cellular infiltrates. Furthermore, prevention of NKT cell activation transiently enhanced the local production of a subset of chemokines, including MIP-2, MCP-1, MIP-1α, and MIP-1β, and altered the relative expression of CD69 and CXCR2 on the surface of both circulating and wound NKT cells. Taken together, these findings suggest that wounding activates NKT cells via CD1d presentation of glycolipid antigen and help further define a role for NKT cells in the regulation of wound inflammation and closure. Many soluble factors have been targeted as potential wound healing therapies, but their clinical success has been limited. Given our findings, the NKT cell may be an attractive target for wound healing therapies.
Department of Surgery, Burn and Shock Trauma Institute, Loyola University Medical Center, Maywood, IL, USA.
David F
DF
Schneider
Jessica L
JL
Palmer
Julia M
JM
Tulley
Elizabeth J
EJ
Kovacs
Richard L
RL
Gamelli
Douglas E
DE
Faunce
D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D018925:Chemokines / Q000378:metabolism; D003872:Dermatitis / Q000276:immunology*; D005434:Flow Cytometry; D008213:Lymphocyte Activation / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D055611:Natural Killer T-Cells / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D023063:Receptors, Interleukin-8B / Q000378:metabolism; D015398:Signal Transduction / Q000276:immunology*; D012867:Skin / Q000166:cytology / Q000276:immunology* / Q000293:injuries*; D013154:Spleen / Q000166:cytology; D014945:Wound Healing / Q000276:immunology*
D000906:Antibodies; D018925:Chemokines; D023063:Receptors, Interleukin-8B