Hydrogen-rich saline provides protection against hyperoxic lung injury.
BACKGROUND
Hydrogen has been proven to be a novel antioxidant through its selectively reducing of the hydroxyl radical. In this study, we investigated the effects of hydrogen-rich saline on the prevention of acute lung injury induced by hyperoxia (HALI) in rats.


METHODS
Physiologic saline, hydrogen-rich saline, or nitrogen-rich saline was administered through intraperitoneal (i.p.) injection during exposure to hyperoxia (10 mL/Kg), respectively.


RESULTS
Severity of HALI was assessed by the volume of pleural effusion, wet-to-dry weight ratio (W/D), and histologic analysis. Apoptosis in lung cells was determined with terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL)-positive staining. The content of pro-inflammatory cytokine interleukin IL-1b and TNF-a in the lung tissues were detected by enzyme-linked immunosorbent assay (ELISA). Hydrogen-rich saline treatment provides protection against HALI by inhibiting lipid, DNA oxidation, and tissue edema. Moreover, hydrogen-rich saline treatment could inhibit apoptosis and inflammation while no significant reduction was observed in nitrogen-rich saline treated animals.


CONCLUSIONS
The results of this study demonstrate that hydrogen-rich saline ameliorated hyperoxia-induced acute lung injury by reducing oxidative stress and inflammatory cascades in lung tissue.
Department of Diving Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai, PR China. sunxjk@hotmail.com
Qiang
Q
Sun
Jianmei
J
Cai
Shulin
S
Liu
Yun
Y
Liu
Weigang
W
Xu
Hengyi
H
Tao
Xuejun
X
Sun
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000818:Animals; D017209:Apoptosis; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000032:analysis; D006859:Hydrogen / Q000627:therapeutic use*; D018496:Hyperoxia / Q000150:complications*; D008168:Lung / Q000473:pathology; D055370:Lung Injury / Q000517:prevention & control*; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D009195:Peroxidase / Q000032:analysis; D011654:Pulmonary Edema / Q000517:prevention & control; D051381:Rats; D017207:Rats, Sprague-Dawley; D012965:Sodium Chloride / Q000627:therapeutic use*; D013482:Superoxide Dismutase / Q000032:analysis
D012965:Sodium Chloride; D008315:Malondialdehyde; D006859:Hydrogen; D000080242:8-Hydroxy-2'-Deoxyguanosine; D009195:Peroxidase; D013482:Superoxide Dismutase; D003849:Deoxyguanosine