Production of lactate in Escherichia coli by redox regulation genetically and physiologically.
Escherichia coli grows fermentatively in glucose-containing medium under anaerobic condition with formation of a mixture of organic acids (lactate, acetate, formate, and succinate) and ethanol to accommodate reducing equivalents generated during glycolysis. In this paper, we tried to improve the lactate accumulation in E. coli by redox regulation genetically and physiologically. Our results indicated that genetic regulation of the host by reducing the reductive by-product may improve the lactate production. In addition, lactate accumulation was also improved under reduced and anaerobic cultivation conditions. Engineered E. coli SDU4 was able to accumulate lactate under strictly anaerobic conditions to 100 g/L with a yield of 1.97 mol/mol glucose.
State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, People's Republic of China.
Huimin
H
Liu
Junhua
J
Kang
Qingsheng
Q
Qi
Guanjun
G
Chen
D000693:Anaerobiosis / Q000187:drug effects; D003470:Culture Media / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism*; D005110:Extracellular Space / Q000187:drug effects / Q000378:metabolism; D005285:Fermentation / Q000187:drug effects; D005818:Genetic Engineering / Q000379:methods*; D005947:Glucose / Q000378:metabolism; D042541:Intracellular Space / Q000187:drug effects / Q000378:metabolism; D019344:Lactic Acid / Q000378:metabolism*; D030781:Organisms, Genetically Modified; D010084:Oxidation-Reduction / Q000187:drug effects
D003470:Culture Media; D019344:Lactic Acid; D005947:Glucose