Design and optimization of non-clogging counter-flow microconcentrator for enriching epidermoid cervical carcinoma cells.
Clogging failure is common for microfilters in living cells concentration; for instance, the CaSki Cell-lines (Epidermoid cervical carcinoma cells) utilizing the flat membrane structure. In order to avoid the clogging, counter-flow concentration units with turbine blade-like micropillar are proposed in microconcentrator design. Due to the unusual geometrical-profiles and extraordinary microfluidic performance, the cells blocking does not occur even at permeate entrances. A counter-flow microconcentrator was designed, with both processing layer and collecting layer arranged in terms of the fractal based honeycomb structure. The device was optimized by coupling Artificial Neuron Network (ANN) and Computational Fluid Dynamics (CFD). The excellent concentration ratio of a final microconcentrator was presented in numerical results.
I.M.S.T./TekMar, Norwegian Center of Expertise on Micro- and Nanotechnology, Vestfold University College, Tønsberg N-3103, Norway.
Nhut
N
Tran-Minh
Tao
T
Dong
Qianhua
Q
Su
Zhaochu
Z
Yang
Henrik
H
Jakobsen
Frank
F
Karlsen
D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D045744:Cell Line, Tumor; D004738:Engineering; D004847:Epithelial Cells / Q000473:pathology; D005260:Female; D005374:Filtration / Q000295:instrumentation*; D006801:Humans; D057446:Hydrodynamics; D055616:Microtechnology / Q000295:instrumentation*; D008954:Models, Biological; D016571:Neural Networks, Computer; D002583:Uterine Cervical Neoplasms / Q000473:pathology*