Physical aspects of COPI vesicle formation.
Coat proteins orchestrate membrane budding and molecular sorting during the formation of transport intermediates. Coat protein complex I (COPI) vesicles shuttle between the Golgi apparatus and the endoplasmic reticulum and between Golgi stacks. The formation of a COPI vesicle proceeds in four steps: coat self-assembly, membrane deformation into a bud, fission of the coated vesicle and final disassembly of the coat to ensure recycling of coat components. Although some issues are still actively debated, the molecular mechanisms of COPI vesicle formation are now fairly well understood. In this review, we argue that physical parameters are critical regulators of COPI vesicle formation. We focus on recent real-time in vitro assays highlighting the role of membrane tension, membrane composition, membrane curvature and lipid packing in membrane remodelling and fission by the COPI coat.
UMR 144 CNRS-Institut Curie, 75248 Paris Cedex 05, France.
Mathieu
M
Pinot
Bruno
B
Goud
Jean-Baptiste
JB
Manneville
D022181:COP-Coated Vesicles / Q000378:metabolism / Q000502:physiology*; D020755:Coat Protein Complex I / Q000378:metabolism / Q000502:physiology*; D055585:Physical Phenomena; D021381:Protein Transport
D020755:Coat Protein Complex I