Intra- and extra-cellular flux distributions of TCA and glyoxalate cycle and vancomycin production of Amycolatopsis orieantalis grown in different glycerol concentration.
The relationship between tricarboxylic acid (TCA) and glyoxalate cycle and the effect of their metabolites levels on the vancomycin production of Amycolatopsis orientalis were investigated in different concentration of glycerol (2.5-20 g/l). Intracellular glycerol levels increased with respect to increases in glycerol concentrations of the growth medium. Extracellular glycerol levels decreased slowly up to 24 h while uptake rates were increased during 36-48 h for 10 and 15 g/l and during 36-60 h at 20 g/l of glycerol. Intracellular citrate, alpha-ketoglutarate, fumarate levels increased up to 10 g/l glycerol concentration. However, intracellular succinate and malate levels were increased up to 15 g/l glycerol. Extracellular citrate, alpha-ketoglutarate, succinate and malate levels increased with respect to increases in glycerol concentration. The highest alpha-ketoglutarate dehydrogenase activity was determined at 15 g/l glycerol. Isocitrate lyase activity showed a positive correlation with the increases in glycerol concentration of the growth medium. Vancomycin production increased with the increases in glycerol concentration from 5 to 10 g/l. These results showed that A. orientalis grown in glycerol containing medium used glyoxalate shunt actively instead of TCA cycle which supports precursors of many amino acid which are effective on the antibiotic production.
University of Dokuz Eylül, Faculty of Science and Art, Department of Chemistry, Biochemistry Division, 35150 Buca, Izmir, Turkey.
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Ayar-Kayali
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Tarhan
D000192:Actinomycetales / Q000254:growth & development / Q000378:metabolism; D002952:Citric Acid Cycle / Q000187:drug effects* / Q000502:physiology; D005990:Glycerol / Q000494:pharmacology*; D006038:Glyoxylates / Q000378:metabolism*; D012997:Solvents / Q000378:metabolism / Q000494:pharmacology*; D014640:Vancomycin / Q000096:biosynthesis*
D006038:Glyoxylates; D012997:Solvents; D014640:Vancomycin; C031150:glyoxylic acid; D005990:Glycerol