In vitro models for the evaluation of angiogenic potential in bone engineering.
Blood vessels have a fundamental role both in skeletal homeostasis and in bone repair. Angiogenesis is also important for a successful bone engineering. Therefore, scaffolds should be tested for their ability to favour endothelial cell adhesion, proliferation and functions. The type of endothelial cell to use for in vitro assays should be carefully considered, because the properties of these cells may depend on their source. Morphological and functional relationships between endothelial cells and osteoblasts are evaluated with co-cultures, but this model should still be standardized, particularly for distinguishing the two cell types. Platelet-rich plasma and recombinant growth factors may be useful for stimulating angiogenesis.
Laboratorio di Fisiopatologia Ortopedica e Medicina Rigenerativa, Istituto Ortopedico Rizzoli, Bologna, Italy. ecenni70@gmail.com
Elisabetta
E
Cenni
Francesca
F
Perut
Nicola
N
Baldini
D000818:Animals; D001842:Bone and Bones / Q000098:blood supply*; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism; D006801:Humans; D018919:Neovascularization, Physiologic; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism; D023822:Tissue Engineering / Q000379:methods*; D042442:Vascular Endothelial Growth Factors / Q000378:metabolism
D042442:Vascular Endothelial Growth Factors