[Inhibitory effect of antisense VEGF₁₂₁ and endostatin genes transfection on tumor growth and metastasis of human giant cell lung cancer].
BACKGROUND
To explore the co-operative inhibitory effect of antisense VEGF gene and endostatin gene transfection on tumor angiogenesis, tumor growth and metastasis of lung cancer.


METHODS
Antisense VEGF₁₂₁ cDNA was transfected into PG cells(PG-AS-VEGF) by lipofectin. After PG-AS-VEGF cells were xenografted to nude mice, PsectagA-endostatin gene was transfected into nude mice by electric pulse mediation. The MVDs in tumors and tumor biological characteristics were observed.


RESULTS
(1)The MVD in PG-AS-VEGF tumor in nude mice was significantly lower than that in PG-vector tumor (PG-AS-VEGF and PG-vector: 40.67±9.35 and 58.34±10.52, respectively) in nude mice. (2)There was no significant difference between the PG-vector tumor and PG-AS-VEGF tumor in early stage of the tumor growth in vivo. However, PG-AS-VEGF tumor grew significantly more slowly than PG-vector tumor after 18 days (P<0.05). (3)PG-AS-VEGF tumor could lead to regional and/or distant lymph node metastases (16.7%, 2/12), which was much more infrequent than that in PG-vector group (50%, 6/12). (4) PG-AS-VEGF tumor growth was remarkably inhibited by endostatin gene transfected at site of the tumor inoculation as compared with the control group in nude mice (P<0.05). (5)The PG-AS-VEGF tumors transfected with the endostatin gene at site of the tumor inoculation(AST) could also produce much lower regional and/or distant lymph node metastases rate (12.5%, 1/8) than that in the PG-AS-VEGF tumor transfected with the PsectagA vector (ASP)(75%, 6/8).


CONCLUSIONS
Endostatin gene transfection could cooperatively inhibit the growth and spontaneous lymph node metastasis of antisense VEGF gene transfected PG cells in nude mice.
Department of Pathology, Peking University, Beijing 100083, P.R.China.
X
X
Wu
J
J
Zheng
J
J
Zhu
J
J
Fu
C
C
Ma
J
J
You
X
X
Cui
J
J
Wang
W
W
Fang
A
A
Zhou
J
J
Tang
B
B
Wu