Efficient in vivo delivery of plasmids and oligonucleotides using hemagglutinating virus of Japan envelope (HVJ-E) vector in immunological disease models.
This unit describes a method for in vivo delivery of oligonucleotides or plasmids using the hemagglutinating virus of Japan envelope (HVJ-E), an inactivated Sendai virus particle, as a delivery system. Viral transfection methods generally show a higher transfection efficiency than nonviral methods for the delivery of genes to cells. However, in using these methods one must bear in mind that the introduction of a virus particle into a host carries a risk for leukemia induction and for creation of disturbances in immune function due to cytotoxicity.
National Institute of Allergy and Infectious Diseases, Bethesda, Maryland, USA.
Atsushi
A
Kitani
Stefan
S
Fichtner-Feigl
D000818:Animals; D005822:Genetic Vectors / Q000009:adverse effects / Q000235:genetics; D006801:Humans; D007938:Leukemia / Q000821:virology; D051379:Mice; D009841:Oligonucleotides / Q000009:adverse effects / Q000235:genetics*; D010957:Plasmids / Q000009:adverse effects / Q000235:genetics*; D029082:Sendai virus / Q000235:genetics*; D014162:Transfection / Q000379:methods*; D014759:Viral Envelope Proteins / Q000235:genetics*
D009841:Oligonucleotides; D014759:Viral Envelope Proteins