Gene expression alterations in immune system pathways in the thymus after exposure to immunosuppressive chemicals.
BACKGROUND
Dysregulation of positive and negative selection, antigen presentation, or apoptosis in the thymus can lead to immunosuppression or autoimmunity. Diethylstilbestrol (DES), dexamethasone (DEX), cyclophosphamide (CPS), and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) are immunosuppressive chemicals that induce similar immunotoxic effects in the thymus, however, the mechanism of toxicity is purported to be different for each compound.


OBJECTIVE
We hypothesized that genomic analysis of thymus after chemical-induced atrophy would yield transcriptional profiles that suggest pathways of toxicity associated with reduced function.


METHODS
Female B6C3F1 mice were exposed to these immunosuppressive agents and changes in gene expression and immune cell subpopulations were evaluated.


RESULTS
All four chemicals induced thymic atrophy and changes in both the relative proportion and absolute number of CD3(+), CD4(+)/CD8(-), CD4(-)/CD8(+), and CD4(+)/CD8(+) thymocytes. The most significant impact of exposure to DEX, DES, and CPS was modulation of gene expression in the T-cell receptor (TCR) complex and TCR and CD28 signaling pathways; this could represent a common mechanism of action and play a pivotal role in lineage commitment and development of T cells. Up-regulation of genes associated with the antigen presentation and dendritic cell maturation pathways was the most distinctive effect of TCDD exposure. These elements, which were also up-regulated by DEX and DES, contribute to positive and negative selection.


CONCLUSIONS
Genomic analysis revealed gene expression changes in several pathways that are commonly associated with xenobiotic-induced immune system perturbations, particularly those that contribute to the development and maturation of thymic T cells.
National Institute of Environmental Health Sciences, National Toxicology Program, Research Triangle Park, North Carolina, USA. frawleyr@niehs.nih.gov
Rachel
R
Frawley
Kimber
K
White
Ronnetta
R
Brown
Deborah
D
Musgrove
Nigel
N
Walker
Dori
D
Germolec
D000818:Animals; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism; D001284:Atrophy / Q000139:chemically induced / Q000378:metabolism; D003520:Cyclophosphamide / Q000633:toxicity; D003907:Dexamethasone / Q000633:toxicity; D004054:Diethylstilbestrol / Q000633:toxicity; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D007107:Immune System / Q000187:drug effects* / Q000378:metabolism; D007166:Immunosuppressive Agents / Q000633:toxicity*; D008285:Major Histocompatibility Complex; D051379:Mice; D000072317:Polychlorinated Dibenzodioxins / Q000633:toxicity; D011948:Receptors, Antigen, T-Cell / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D013601:T-Lymphocytes / Q000378:metabolism; D013950:Thymus Gland / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D015854:Up-Regulation / Q000235:genetics
D015703:Antigens, CD; D007166:Immunosuppressive Agents; D000072317:Polychlorinated Dibenzodioxins; D011948:Receptors, Antigen, T-Cell; D004054:Diethylstilbestrol; D003907:Dexamethasone; D003520:Cyclophosphamide