Bovine herpesvirus 4 based vector as a potential oncolytic-virus for treatment of glioma.
The application of gene therapy for malignant gliomas is still under study and the use of specific vectors represents an important contribution. Here, we investigated bovine herpesvirus 4 (BoHV-4), which is non-pathogenic if injected into the rodent brain. We show that the vector can infect mouse, rat and human glioma cell lines and primary cultures obtained from human glioblastoma in vitro. BoHV-4 was injected into a tumour grown in rat brain. Although virus expression was scattered across the tumour mass, it was mainly located in the peripheral area of larger gliomas. These data support BoHV-4 as a candidate vector for glioma treatment.
Department of Animal Health, University of Parma, Italy.
Marco
M
Redaelli
Carla
C
Mucignat-Caretta
Andrea
A
Cavaggioni
Antonio
A
Caretta
Domenico
D
D'Avella
Luca
L
Denaro
Sandro
S
Cavirani
Gaetano
G
Donofrio
D000818:Animals; D001921:Brain / Q000821:virology; D002478:Cells, Cultured; D005822:Genetic Vectors; D005910:Glioma / Q000628:therapy*; D026021:Herpesvirus 4, Bovine / Q000235:genetics*; D006801:Humans; D051379:Mice; D050504:Oncolytic Viruses / Q000235:genetics* / Q000472:pathogenicity*; D051381:Rats; D014774:Virulence