Purification and structural characterization of a new water-soluble neutral polysaccharide GLP-F1-1 from Ganoderma lucidum.
A water-soluble neutral polysaccharide (GLP-F1-1) was isolated from the fruiting bodies of Ganoderma lucidum by DEAE Sepharose Fast Flow and Sephacryl S-500 High Resolution Chromatography. The neutral polysaccharide had an average molecular weight (Mw) of approximately 2.5×10(6) kDa. GC analysis showed that this polysaccharide was mainly composed of glucose and galactose in the molar ratio of 34:1. 1H and 13C NMR spectroscopy in combination with GC-MS technique indicated that the new polysaccharide had a backbone chain of 1,4-disubstituted-β-glucoseopyranose and 1,4,6-trisubstituted-β-glucoseopyranosyl, while the branched chains were mainly composed of 1,6-disubstituted-β-glucopyranosyl and 1,4-disubstituted-β-galactoseopyranosyl residues.
College of Light Industry and Food Sciences, South China University of Technology, Guangdong Province, Guangzhou 510640, China. huangfeihong0220@sina.com
Sheng-quan
SQ
Huang
Jin-wei
JW
Li
Yu-qiang
YQ
Li
Zhou
Z
Wang
D005591:Chemical Fractionation; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D008401:Gas Chromatography-Mass Spectrometry; D009682:Magnetic Resonance Spectroscopy; D008970:Molecular Weight; D009005:Monosaccharides / Q000032:analysis; D011134:Polysaccharides / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification*; D020940:Reishi / Q000737:chemistry*; D012995:Solubility; D017550:Spectroscopy, Fourier Transform Infrared; D014867:Water / Q000737:chemistry*
D009005:Monosaccharides; D011134:Polysaccharides; D014867:Water