A variable gene delivery carrier--biotinylated chitosan/polyethyleneimine.
A variable gene delivery system has been developed based on conjugating chitosan to biotin through a functionalized poly(ethylene glycol) (PEG) spacer, which can be used to further bind different molecules on the outer layer of a polymer/DNA complex by streptavidin (SA)-biotin linkage. In this study, TAT-conjugated SA was used as the model molecule to prove the conjugation function of the prepared complex. In addition, low-molecular-weight poly(ethyleneimine) (PEI) was added into the polymer/DNA complex to increase the transfection efficiency. The results of the luciferase assay show that the transfection efficiency of the prepared complex was significantly correlated with the amount of PEI and was further enhanced when TAT was conjugated to the complex by SA-biotin linkage. Considered to have negligible cytotoxic effects, the variable gene delivery complex prepared in this study would be of considerable potential as carriers for in vitro applications.
Institute of Polymer Science and Engineering, College of Engineering, National Taiwan University, Taipei 106, Taiwan, Republic of China.
Yi-Chen
YC
Chung
Fu-Hsiung
FH
Chang
Ming-Feng
MF
Wei
Tai-Horng
TH
Young
D019921:Biotinylation; D048271:Chitosan / Q000737:chemistry*; D004247:DNA / Q000008:administration & dosage / Q000737:chemistry* / Q000235:genetics*; D004337:Drug Carriers / Q000138:chemical synthesis*; D004339:Drug Compounding / Q000379:methods; D008422:Materials Testing; D011094:Polyethyleneimine / Q000737:chemistry*; D014162:Transfection / Q000379:methods*
D004337:Drug Carriers; D011094:Polyethyleneimine; D004247:DNA; D048271:Chitosan