Spectroscopic studies on the interaction of quercetin-terbium(III) complex with calf thymus DNA.
The interaction of native calf thymus DNA (CT-DNA) with quercetin-terbium(III) [Q-Tb(III)] complex at physiological pH was monitored by UV absorption spectrophotometry, circular dichroism, fluorescence spectroscopy, and viscosimetric techniques. The complex displays binding properties to the CT-DNA and was found to interact with CT-DNA through outside binding, demonstrated by a hypochromic effect of Q-Tb(III) on the UV spectra of CT-DNA and the calculated association constants (K). Also, decrease in the specific viscosity of CT-DNA, decrease in the fluorescence intensity of Q-Tb(III) solutions in the presence of increasing amounts of CT-DNA, and detectable changes in the circular dichroism spectrum of CT-DNA are other evidences to indicate that Q-Tb(III) complex interact with CT-DNA through outside binding.
Department of Plant Biology, Faculty of Natural Science, University of Tabriz, Tabriz, Iran. gdehghan@tabrizu.ac.ir
Gholamreza
G
Dehghan
Jafar Ezzati Nazhad
JE
Dolatabadi
Abolghasem
A
Jouyban
Karim Asadpour
KA
Zeynali
Seyed Mojtaba
SM
Ahmadi
Soheila
S
Kashanian
D000465:Algorithms; D000818:Animals; D001667:Binding, Competitive; D002417:Cattle; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D007700:Kinetics; D015394:Molecular Structure; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D011794:Quercetin / Q000737:chemistry* / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013057:Spectrum Analysis / Q000379:methods*; D013725:Terbium / Q000737:chemistry* / Q000378:metabolism; D014783:Viscosity
D009942:Organometallic Compounds; D013725:Terbium; D004247:DNA; C427203:calf thymus DNA; D011794:Quercetin