[The research of thrombolytic and hemolysis effect from Eupolyphage fibrinolyric protein and its inhibitory effect on S180 ascites tumor of mice].
OBJECTIVE
Eupolyphage fibrinolyric protein (EFP) was isolated and purified from Eupolyphage sineses, and its thrombolytic effect, hemolysis effect and inhibitory effect on S180 ascites tumor were investigated.


METHODS
EFP was isolated and purified by ammonium precipitation and DEAE ion exchange chromatography. It's thrombolytic and hemolysis effect were determined. MTT method and Colony-forming method were used to determine the inhibitory effect on S180 ascites tumor.


RESULTS
the EFP was proved to have the effect of Thrombolytic and Hemolysis, and both increased dose-dependently, however at a lower concentration, the EFP had no hemocytolysis. The EFP was also proved the effect of inhibitory on cell proliferation and Colony-forming on S180 ascites tumor of Mice.


CONCLUSIONS
EFP has a strong thrombolytic activity and weak hemolytic, and has inhibitory effect on S180 ascites tumor of mice.
Faculty of light and Chemical, Guangdong University of Technology, Guangzhou 510006, China.
Zi-Wei
ZW
Li
Ya-Li
YL
Han
Hao
H
Liu
Hong
H
Ding
D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000494:pharmacology*; D020048:Blattellidae / Q000737:chemistry*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005260:Female; D005341:Fibrinolysin / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D005343:Fibrinolytic Agents / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D008419:Materia Medica / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D051379:Mice; D012510:Sarcoma 180 / Q000473:pathology*
D000970:Antineoplastic Agents; D005343:Fibrinolytic Agents; D008419:Materia Medica; D005341:Fibrinolysin