Characterization of garvicin ML, a novel circular bacteriocin produced by Lactococcus garvieae DCC43, isolated from mallard ducks (Anas platyrhynchos).
Lactococcus garvieae DCC43 produces a bacteriocin, garvicin ML (GarML), with a molecular mass of 6,004.2 Da. Data from de novo amino acid sequencing by tandem mass spectrometry and nucleotide sequencing by reverse genetics suggested that the bacteriocin is synthesized as a 63-amino-acid precursor with a 3-amino-acid leader peptide that is removed by cleavage. Subsequently, a covalent linkage between the N and C termini forms the mature version of this novel 60-amino-acid circular bacteriocin.
Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad Complutense de Madrid (UCM), Madrid, Spain.
Juan
J
Borrero
Dag A
DA
Brede
Morten
M
Skaugen
Dzung B
DB
Diep
Carmen
C
Herranz
Ingolf F
IF
Nes
Luis M
LM
Cintas
Pablo E
PE
Hernández
D000818:Animals; D001430:Bacteriocins / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004372:Ducks / Q000382:microbiology*; D017045:Lactococcus / Q000302:isolation & purification* / Q000378:metabolism*; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D008970:Molecular Weight; D017422:Sequence Analysis, DNA
D001430:Bacteriocins; D004269:DNA, Bacterial