Cytokine gene polymorphisms in egyptian cases with brain tumors.
BACKGROUND
Cytokines are proposed to play important roles in brain tumor biology as well as neurodegeneration or impaired neuronal function.


OBJECTIVE
This work aimed to check the association of polymorphisms of cytokine genes in Egyptian cases with brain tumors.


METHODS
This work included 45 cases affected by brain tumors diagnosed as 24 benign and 21 malignant. Their median age was 45 years, and they were 20 males and 25 females. These cases were taken randomly from the Neurosurgery Department of Mansoura University Hospital, Egypt. Case genotypes were compared to 98 healthy unrelated controls from the same locality. DNA was amplified using PCR utilizing sequence specific primers (SSP) for detection of polymorphisms related to TNF-a-308 (G/A), IL-10-1082 (G/A), IL-6-174 (G/C) and IL-1Ra (VNTR) genes.


RESULTS
Cases affected with benign brain tumors showed a significant higher frequency of IL-10-1082 A/A [odds ratio (OR=8.0), p<0.001] and IL-6-174 C/C (OR=6.3, p=0.002) homozygous genotypes as compared to controls. Malignant cases, on the other hand, showed significantly higher frequency of IL-6-174 C/C (OR =4.8, p=0.002) homozygous genotype and TNF-a-308 A/A (OR=4.9, p<0.001) homozygous genotype when compared to controls. In the meantime, all cases showed no significant difference regarding the distribution of IL-1Ra VNTR genotype polymorphism compared to controls.


CONCLUSIONS
Cytokine gene polymorphisms showed a pattern of association with brain tumors which may have potential impact on family counseling and disease management.
The Department of Zoology Cell Biology, Mansoura University, Mansoura, Egypt. na_ri_eg@yahoo.com
Nariman K
NK
Badr El-Din
Ahmad
A
Settin
Nabil
N
Ali
El-Sayed K
el-SK
Abdel-Hady
Fatma K
FK
Salem
D014408:Biomarkers, Tumor / Q000235:genetics*; D001932:Brain Neoplasms / Q000235:genetics*; D016022:Case-Control Studies; D016207:Cytokines / Q000235:genetics*; D004247:DNA / Q000235:genetics; D004534:Egypt; D005260:Female; D005838:Genotype; D006801:Humans; D053590:Interleukin 1 Receptor Antagonist Protein / Q000235:genetics; D016753:Interleukin-10 / Q000235:genetics; D015850:Interleukin-6 / Q000235:genetics; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D011379:Prognosis; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
D014408:Biomarkers, Tumor; D016207:Cytokines; D053590:Interleukin 1 Receptor Antagonist Protein; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D004247:DNA