Efficient purification with high recovery of vanadium bromoperoxidase from Corallina officinalis.
A novel, simple and highly efficient process for purifying vanadium bromoperoxidase from Corallina officinalis is reported. The key innovation is adding 0.5 mM sodium orthovanadate to the crude cell extract followed by heating at 70°C for 2 h, by which a 5.4-fold purification with a 100% activity recovery was achieved. Combining the heat treatment with ammonium sulfate precipitation and DEAE-52 column chromatography, the overall yield was 84%, 3.8 times greater than the highest yield previously reported. Finally, a specific activity of 310 U/mg, a 27-fold purification of the crude enzyme solution was produced.
Marine Bioproducts Engineering Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China.
Biaoming
B
Zhang
Xupeng
X
Cao
Xiaofei
X
Cheng
Peichun
P
Wu
Tonghu
T
Xiao
Wei
W
Zhang
D011232:Chemical Precipitation; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D010544:Peroxidases / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D000461:Rhodophyta / Q000235:genetics / Q000378:metabolism*
D010544:Peroxidases; C026195:bromide peroxidase