The crosstalks between adipokines and catecholamines.
Adipocytes, which secrete a spectrum of adipokines, play an integral role in metabolism via communications with other endocrine cells. In the present work, we have studied the interplays between adipokines and catecholamines, using 3T3-L1 adipocytes and PC12 cells as the cell models and an integrative experimental platform. We demonstrate that all catecholamines inhibit vesicle trafficking and secretion of leptin and resistin through β-adrenergic receptors, while leptin and resistin enhance the vesicle trafficking and secretion of catecholamines through PKC, PKA, MAPK kinase and Ca(2+) dependent pathways. The crosstalks between adipokines and catecholamines were further corroborated by co-culturing 3T3-L1 adipocytes and PC12 cells. Our findings highlight the importance of adipo-adrenal axis in energy metabolism and the intricate interactions between metabolic hormones.
Division of Bioengineering, Nanyang Technological University, 70 Nanyang Drive, Singapore 637457, Singapore.
Aung
A
Than
Feng
F
Ye
Renhao
R
Xue
Jun Wei
JW
Ong
Chueh Loo
CL
Poh
Peng
P
Chen
D041721:3T3-L1 Cells; D054392:Adipokines / Q000378:metabolism*; D000818:Animals; D002395:Catecholamines / Q000378:metabolism*; D018920:Coculture Techniques; D022162:Cytoplasmic Vesicles / Q000378:metabolism; D005089:Exocytosis / Q000502:physiology; D020738:Leptin / Q000378:metabolism; D008561:Membrane Fusion; D051379:Mice; D016716:PC12 Cells; D051381:Rats; D011943:Receptors, Adrenergic, beta / Q000378:metabolism; D052243:Resistin / Q000378:metabolism
D054392:Adipokines; D002395:Catecholamines; D020738:Leptin; D011943:Receptors, Adrenergic, beta; D052243:Resistin