New aspects of DNA-based authentication of Chinese medicinal plants by molecular biological techniques.
DNA technology provides a powerful tool to complement chemical analyses for authentication of Chinese medicinal plants and to ensure that herbal materials are not contaminated with ineffective or potentially harmful substitutes or adulterants. In the last two decades molecular biotechnology has provided sophisticated molecular techniques for authentication of botanical materials at the DNA level. This review provides an account of the most commonly used DNA-based technologies (RAPD, RFLP, ARMS, CAPS, AFLP, DAF, ISSR, SSR, sequencing, hybridization and microarrays) including suitable examples of Chinese medical plants. A critical evaluation of all methods is presented concerning sensitivity, reliability, reproducibility, and running costs. Recent achievements in the field of DNA barcoding and DNA chip technology that offer great potentials for screening of DNA and emerging new developments for future identification of species are briefly outlined.
Biology Department I-Systematic Botany, Ludwig-Maximilians University Munich, Metzingerstrasse 67, Munich, Germany. heubl@lrz.uni-muenchen.de
Günther
G
Heubl
D054458:Amplified Fragment Length Polymorphism Analysis / Q000379:methods; D016172:DNA Fingerprinting; D018744:DNA, Plant / Q000032:analysis; D005821:Genetic Techniques; D018895:Microsatellite Repeats; D009693:Nucleic Acid Hybridization / Q000379:methods; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D010946:Plants, Medicinal / Q000145:classification* / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods; D017422:Sequence Analysis, DNA
D018744:DNA, Plant