[Effect of celecoxib and adriamycin on proliferation and apoptosis of Raji cells and its mechanism].
OBJECTIVE
To assess the effect of celecoxib in combination with adriamycin(ADM) on cell proliferation and apoptosis.


METHODS
Cell lines were treated with celecoxib,and cell proliferation was examined by the method of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) at different time. The cellular apoptotic rate was analyzed by flow cytometry. RT-PCR was applied to distinguish the level of caspase-9 mRNA expression. Immunohistochemistry was used to detect the caspase-9 protein expression in Raji cells.


RESULTS
Celecoxib inhibited the proliferation and increased the apoptosis of Raji cell lines in a dose-dependent and time-dependent manner within 20-100 μmol/L. Compared with the celecoxib alone, the proliferation of Raji cell lines incubated with celecoxib and adriamycin was decreased. Caspase-9 mRNA and protein expression in Raji cells were significantly enhanced after the treatment of celecoxib (P<0.05).


CONCLUSIONS
Celecoxib can inhibit the proliferation of Raji cells in a dose-dependent and time-dependent manner. Celecoxib may lead to the apoptosis of Raji cells by up-regulating activities of caspase-9. Adriamycin could intensify the effect.
Department of Hematology, Xiangya Hospital, Central South University, Changsha 410008, China.
Xiaochun
X
Huang
Xiaolin
X
Li
Ranxin
R
Huang
Xiaogang
X
Yang
Bin
B
Fu
D017209:Apoptosis / Q000187:drug effects*; D002051:Burkitt Lymphoma / Q000473:pathology*; D053453:Caspase 9 / Q000235:genetics / Q000378:metabolism; D000068579:Celecoxib; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004317:Doxorubicin / Q000494:pharmacology*; D004357:Drug Synergism; D006801:Humans; D011720:Pyrazoles / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013449:Sulfonamides / Q000494:pharmacology*
D011720:Pyrazoles; D012333:RNA, Messenger; D013449:Sulfonamides; D004317:Doxorubicin; C506433:CASP9 protein, human; D053453:Caspase 9; D000068579:Celecoxib