Biophysical investigation of pulmonary surfactant surface properties upon contact with polymeric nanoparticles in vitro.
Nanoparticulate drug carriers have been proposed for the targeted and controlled release of pharmaceuticals to the lung. However, inhaled particles may adversely affect the biophysical properties of pulmonary surfactant. This study examines the influence of polymeric nanoparticles with distinct physicochemical properties on the adsorption and dynamic surface tension lowering properties of pulmonary surfactant. Nanoparticles had a mean size of 100 nm with narrow size distributions. Although poly(styrene) and poly(D,L-lactide-co-glycolide) nanoparticles revealed a dose-dependent influence on biophysics of pulmonary surfactant, positively-charged nanoparticles made from poly(butyl methacrylate-co-(2-dimethylaminoethyl) methacrylate-co-methyl methacrylate) showed no effect. This behavior is attributed to the differences in ζ-potential and surface hydrophobicity, which in turn involves an altered adsorption pattern of the positively charged surfactant proteins to the nanoparticles. This study suggests that polymeric nanoparticles do not substantially affect the biophysical properties of pulmonary surfactant and may be a viable drug-delivery vehicle for the inhalative treatment of respiratory diseases.


BACKGROUND
Inhaled nanoparticulate drug carriers may adversely affect the biophysical properties of pulmonary surfactant. In this study the influence of polymeric nanoparticles was characterized from this standpoint, with the conclusion that polymeric nanoparticles do not substantially affect the biophysical properties of pulmonary surfactant and may be viable drug-delivery vehicles for inhalational treatment.
Medical Clinic II, Department of Internal Medicine, Justus-Liebig-Universität, Giessen, Germany.
Moritz
M
Beck-Broichsitter
Clemens
C
Ruppert
Thomas
T
Schmehl
Andreas
A
Guenther
Thomas
T
Betz
Udo
U
Bakowsky
Werner
W
Seeger
Thomas
T
Kissel
Tobias
T
Gessler
D000179:Acrylates / Q000737:chemistry; D000327:Adsorption; D000818:Animals; D055592:Biophysical Phenomena; D002417:Cattle; D045304:Cytochromes c / Q000378:metabolism; D019344:Lactic Acid / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry*; D010743:Phospholipids / Q000737:chemistry*; D011100:Polyglycolic Acid / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000737:chemistry*; D011137:Polystyrenes / Q000737:chemistry; D037661:Pulmonary Surfactant-Associated Proteins / Q000378:metabolism; D013500:Surface Tension; D013816:Thermodynamics
D000179:Acrylates; C477020:Eudragit E100; D010743:Phospholipids; D011108:Polymers; D011137:Polystyrenes; D037661:Pulmonary Surfactant-Associated Proteins; C078272:SF-RI 1, bovine surfactant preparation; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D045304:Cytochromes c