Characterization of a novel rice kinesin O12 with a calponin homology domain.
Genomic analysis predicted that the rice (Oryza sativa var. japonica) genome encodes at least 41 kinesin-like proteins including the novel kinesin O12, which is classified as a kinesin-14 family member. O12 has a calponin homology (CH) domain that is known as an actin-binding domain. In this study, we expressed the functional domains of O12 in Escherichia coli and determined its enzymatic characteristics compared with other kinesins. The microtubule-dependent ATPase activity of recombinant O12 containing the motor and CH domains was significantly reduced in the presence of actin. Interestingly, microtubule-dependent ATPase activity of the motor domain was also affected by actin in the absence of the CH domain. Our findings suggest that the motor activity of the rice plant-specific kinesin O12 may be regulated by actin.
Division of Bioengineering, Graduate School of Engineering, Soka University, Hachioji, Tokyo 192-8577, Japan.
Nozomi
N
Umezu
Nobuhisa
N
Umeki
Toshiaki
T
Mitsui
Kazunori
K
Kondo
Shinsaku
S
Maruta
D000199:Actins / Q000737:chemistry; D000244:Adenosine Diphosphate / Q000737:chemistry; D000251:Adenosine Triphosphatases / Q000737:chemistry; D000255:Adenosine Triphosphate / Q000737:chemistry; D002135:Calcium-Binding Proteins / Q000235:genetics; D016547:Kinesins / Q000737:chemistry* / Q000145:classification / Q000235:genetics; D008840:Microfilament Proteins / Q000235:genetics; D008958:Models, Molecular; D012275:Oryza; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry* / Q000235:genetics; D011485:Protein Binding; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry* / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D000096985:Calponins
D000199:Actins; D000244:Adenosine Diphosphate; D000251:Adenosine Triphosphatases; D000255:Adenosine Triphosphate; D002135:Calcium-Binding Proteins; D016547:Kinesins; D008840:Microfilament Proteins; D010940:Plant Proteins; D011994:Recombinant Proteins; D000096985:Calponins