Crystallization and preliminary X-ray analysis of a marine diatom-infecting single-stranded RNA virus.
Crystals of a diatom-infecting virus (CtenRNAV) that diffracted to a resolution of 4.0 Å were grown in a mixture of 2-methyl-2,4-pentanediol (MPD), calcium chloride and sodium acetate. It was possible to freeze the crystals directly at liquid-nitrogen temperature as the reservoir solution, which included about 30% MPD, acted as a cryoprotectant during X-ray diffraction data collection. A data set was collected from a single frozen crystal obtained using this method. The crystals belonged to space group P6(3)22, with unit-cell parameters a = b = 448.67, c = 309.76 Å and two virus particles in the unit cell. The virus-particle orientation was determined using a rotation function and the virus-particle centre was estimated on the basis of crystallographic considerations. The packing of CtenRNAV in the crystal lattice was revealed by this preliminary crystallographic study.
RIKEN SPring-8 Center/RIKEN Harima Institute, 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo 679-5148, Japan. naitow@spring8.or.jp
Hisashi
H
Naitow
Yoko
Y
Shirai
Yuji
Y
Tomaru
Keizo
K
Nagasaki
D003460:Crystallization; D018360:Crystallography, X-Ray; D017377:Diatoms / Q000821:virology; D012328:RNA Viruses / Q000737:chemistry*; D014764:Viral Proteins / Q000737:chemistry*; D014771:Virion / Q000737:chemistry
D014764:Viral Proteins