Micro-optical sectioning tomography to obtain a high-resolution atlas of the mouse brain.
The neuroanatomical architecture is considered to be the basis for understanding brain function and dysfunction. However, existing imaging tools have limitations for brainwide mapping of neural circuits at a mesoscale level. We developed a micro-optical sectioning tomography (MOST) system that can provide micrometer-scale tomography of a centimeter-sized whole mouse brain. Using MOST, we obtained a three-dimensional structural data set of a Golgi-stained whole mouse brain at the neurite level. The morphology and spatial locations of neurons and traces of neurites could be clearly distinguished. We found that neighboring Purkinje cells stick to each other.
Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan 430074, P. R. China.
Anan
A
Li
Hui
H
Gong
Bin
B
Zhang
Qingdi
Q
Wang
Cheng
C
Yan
Jingpeng
J
Wu
Qian
Q
Liu
Shaoqun
S
Zeng
Qingming
Q
Luo
D000818:Animals; D001921:Brain / Q000033:anatomy & histology* / Q000166:cytology*; D001931:Brain Mapping / Q000379:methods*; D007091:Image Processing, Computer-Assisted; D021621:Imaging, Three-Dimensional; D008297:Male; D051379:Mice; D008853:Microscopy; D008867:Microtomy; D009434:Neural Pathways; D016501:Neurites / Q000648:ultrastructure; D009474:Neurons / Q000166:cytology*; D041622:Tomography, Optical