CDKN1B/p27 expression in peripheral T cell lymphoma not otherwise specified.
OBJECTIVE
Peripheral T cell lymphoma not otherwise specified (PTCL/NOS) is the commonest PTCL subtype. Recently, proliferation pathways have been found to be commonly altered in PTCL/NOS. CDKN1B/p27, a critical regulator of cell cycle and proliferation, has been suggested to be involved in T cell lymphomagenesis. This study aimed to evaluate the possible occurrence of CDKN1B/p27 aberrations in PTCL/NOS.


METHODS
CDKN1B/p27 expression at RNA and protein level by DNA and tissue microarrays, in 28 and 98 cases, respectively, was studied. Additionally, direct sequencing of CDKN1B in 81 PTCL/NOS was performed.


RESULTS
CDKN1B mRNA was similarly expressed in PTCL/NOS and normal T lymphocytes. In addition, structural abnormalities were not found; these included mutations and deletions in any exons, exon-intron junctions or regulatory regions. Furthermore, physiological expression of p27 in neoplastic cells was demonstrated by immunohistochemistry; this was mutually exclusive with Ki-67, as expected when the system is intact. Consistently, the expression of other molecules that are functionally related to CDKN1B/p27 in controlling cell cycle (including CCNE1) did not appear to be affected at either the mRNA or protein level. Finally, it was found that p27 expression was not significantly related with overall survival.


CONCLUSIONS
CDKN1B/p27 aberrations seem to be uncommon in PTCL/NOS pathogenesis.
Department of Haematology and Oncological Sciences L and A Seràgnoli, S. Orsola-Malpighi Hospital, University of Bologna, Bologna, Italy.
Anna
A
Gazzola
Maria Teresa
MT
Sista
Claudio
C
Agostinelli
Simona
S
Righi
Maria Rosaria
MR
Sapienza
Claudia
C
Mannu
Maura
M
Rossi
Francesco
F
Bacci
Elena
E
Sabattini
Philip
P
Went
Pier Luigi
PL
Zinzani
Stefano A
SA
Pileri
Pier Paolo
PP
Piccaluga
D050760:Cyclin-Dependent Kinase Inhibitor p27; D015870:Gene Expression; D020869:Gene Expression Profiling / Q000379:methods; D018816:Genes, cdc / Q000502:physiology; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D016411:Lymphoma, T-Cell, Peripheral / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D020641:Polymorphism, Single Nucleotide; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics; D012334:RNA, Neoplasm / Q000235:genetics; D016019:Survival Analysis; D016176:T-Lymphocyte Subsets / Q000378:metabolism
C436291:CDKN1B protein, human; D047908:Intracellular Signaling Peptides and Proteins; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D050760:Cyclin-Dependent Kinase Inhibitor p27