[Biologic effect of transforming growth factor-β1 on urethra cells cultured in vitro].
OBJECTIVE
To investigate the effects of transforming growth factor-β1 (TGF-β1) on growth controlling and the expression of connective tissue growth factor mRNA(CTGF mRNA) in urethra epithelium cells and fibroblasts cultured in vitro.


METHODS
Urethra epithelial cells and fibroblasts were cultured in vitro and identified. The fourth generation cells were divided into control group (cultured by cell medium without TGF-β1) and experimental groups(cultured by cell medium containing TGF-β1 1, 2, 4 and 8 µg/L), the vital force of cells were examined by MTT and cell counting, the expression of CTGF mRNA were examined by RT-PCR after 24 hours.


RESULTS
The optical density and cell count decreased in experimental groups of urethra epithelium cells and increased in experimental groups of fibroblasts with the concentration of TGF-β1 being heightened compared with the control group (P < 0.05). The expression of CTGF mRNA increased with the heightening concentration of TGF-β1 in all experimental groups of urethra epithelium cells and fibroblasts by RT-PCR (P < 0.05).


CONCLUSIONS
TGF-β1 can inhibit the growth of urethra epithelium cells and promote the growth of fibroblasts in vitro, it can induce the expression of CTGF mRNA in two cells above-mentioned.
Department of Plastic Surgery, Henan Provincial People's Hospital, Zhengzhou 450003, China. zhaihongfeng@sina.com
Hong-feng
HF
Zhai
Lin-gang
LG
Xu
Zai-lan
ZL
Guo
Chang-hong
CH
Qiu
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D055513:Connective Tissue Growth Factor / Q000235:genetics / Q000378:metabolism*; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D009092:Mucous Membrane / Q000166:cytology; D012333:RNA, Messenger / Q000235:genetics; D011817:Rabbits; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology*; D014521:Urethra / Q000166:cytology*
D012333:RNA, Messenger; D053773:Transforming Growth Factor beta1; D055513:Connective Tissue Growth Factor