Fluorogenic assay and live cell imaging of HIV-1 protease activity using acid-stable quantum dot-peptide complex.
A novel QD-peptide complex for detecting HIV-1 protease activity was prepared from simple one step electrostatic interaction. Fluorescence recovery of the pre-quenched QD through fluorescence resonance energy transfer allowed for in vitro assay and live cell imaging of the protease activity in HIV-1 transfected cells, proving the potential for cell-based protease inhibitor screening.
Nano/Bio Chemistry Laboratory, Institut Pasteur Korea (IP-K), 696 Sampyeong-dong, Bundang-gu, Seongnam-Si, Gyeonggi-Do, 464-400, South Korea.
Youngseon
Y
Choi
Junghan
J
Lee
Keumhyun
K
Kim
Heeyeon
H
Kim
Peter
P
Sommer
Rita
R
Song
D003952:Diagnostic Imaging / Q000379:methods*; D005456:Fluorescent Dyes / Q000032:analysis; D016333:HIV Protease / Q000378:metabolism*; D006801:Humans; D010455:Peptides; D045663:Quantum Dots
D005456:Fluorescent Dyes; D010455:Peptides; D016333:HIV Protease; C514803:p16 protease, Human immunodeficiency virus 1