Study of the molecular recognition of aptamers selected through ovarian cancer cell-SELEX.
BACKGROUND
Ovarian cancer is the most lethal gynecological malignancy, and the ovarian clear cell carcinoma subtype (OCCA) demonstrates a particularly poor response to standard treatment. Improvements in ovarian cancer outcomes, especially for OCCA, could be expected from a clearer understanding of the molecular pathology that might guide strategies for earlier diagnosis and more effective treatment.


RESULTS
Cell-SELEX technology was employed to develop new molecular probes for ovarian cancer cell surface markers. A total of thirteen aptamers with K(d)'s to ovarian cancer cells in the pico- to nanomolar range were obtained. Preliminary investigation of the targets of these aptamers and their binding characteristics was also performed.


CONCLUSIONS
We have selected a series of aptamers that bind to different types of ovarian cancer, but not cervical cancer. Though binding to other cancer cell lines was observed, these aptamers could lead to identification of biomarkers that are related to cancer.
Department of Chemistry, Center for Research at Bio/Nano Interface, University of Florida, Gainesville, Florida, United States of America.
Dimitri
D
Van Simaeys
Dalia
D
López-Colón
Kwame
K
Sefah
Rebecca
R
Sutphen
Elizabeth
E
Jimenez
Weihong
W
Tan
D052157:Aptamers, Nucleotide / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D001667:Binding, Competitive; D014408:Biomarkers, Tumor / Q000378:metabolism*; D045744:Cell Line, Tumor; D005260:Female; D005434:Flow Cytometry; D045325:HCT116 Cells; D019073:HT29 Cells; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D010051:Ovarian Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D052156:SELEX Aptamer Technique / Q000379:methods*; D013696:Temperature
D052157:Aptamers, Nucleotide; D014408:Biomarkers, Tumor