[Study on the growth of H1299 cell line transfected transiently with mouse p53 minigenes with different codon 172 structures].
BACKGROUND
To investigate the effect on cell growth after transfected with mouse 172 wild-type p53 (Arg), pseudo-wild-type p53 (Arg->Leu) and mutant p53 (Arg->His).


METHODS
Vectors of three kinds of p53 minigene were transfected into p53-null H1299 cells through lipofectAMINE. Cells were collected at different time after transfection and analyzed by cell counting, flow cytometry and Western blotting.


RESULTS
Wild-type p53 and pseudo-wild-type p53 could induce cell apoptosis and cell cycle arrest at different time. Bax, p21(WAF1) and Mdm-2 were transactivated by wild-type p53 and pseudo-wild-type p53. No tumor growth suppression was observed in mutant p53 transfected cells.


CONCLUSIONS
Wild-type p53 and pseudo-wild-type p53 can inhibit cell growth by inducing apoptosis and cell cycle arrest through transactivation of Bax and p21(WAF1). There exists feedback loop between Mdm-2 and p53. The different biological effect between 172Leu and 172His shows that some p53 variants such as 172 Arg->Leu may act as wild-type p53.
Department of Pathology, Peking University, Beijing 100083, P.R.China.
Y
Y
Ma
B
B
Wu
J
J
Liu
J
J
You
X
X
Cui
J
J
Wang
P
P
Hui