LC-MS determination and pharmacokinetics of p-coumaric acid in rat plasma after oral administration of p-coumaric acid and freeze-dried red wine.
A sensitive and efficient liquid chromatography-mass spectrometry (LC-MS) method was developed and validated for the determination of p-coumaric acid (CA) in rat plasma. After addition of the internal standard (IS) hydrochlorothiazide and acidification with 2 M hydrochloric acid, plasma samples were extracted by ethyl acetate and separated on a Kromasil C18 column (200 mm × 4.6 mm, 5 μm) using a mobile phase composed of methanol-0.5‰ acetic acid (60:40, v/v) within a runtime of 6.0 min. Analysis was performed in selected ion monitoring (SIM) mode with a negative electrospray ionization (ESI) interface. The target ions were m/z 163.15 for CA and m/z 295.95 for IS. The linear range was 0.01-15 μg·mL(-1), and the lower limit of quantification (LLOQ) was 0.01 μg·mL(-1). The intraday and interday precision (RSD %) were lower than 10% and accuracy (RE%) ranged from 97.1 to 103.2%. The validated method was successfully applied to the comparative pharmacokinetic study of CA in rat plasma after oral administration of CA and freeze-dried red wine, respectively. It was found that both AUC and T1/2 of CA in freeze-dried red wine were increased significantly (p < 0.05) compared with that in monomer. In addition, a double-peak profile could be observed from the concentration-time curve after oral administration of freeze-dried red wine.
School of Pharmacy, Shenyang Pharmaceutical University, Wenhua Road 103, Shenyang 110016, P. R. China.
Yan
Y
Cui
Qing
Q
Li
Ming
M
Zhang
Zhenzhen
Z
Liu
Weidong
W
Yin
Wentao
W
Liu
Xiaohui
X
Chen
Kaishun
K
Bi
D000284:Administration, Oral; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003373:Coumaric Acids / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics*; D011422:Propionates; D051381:Rats; D017208:Rats, Wistar; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D014920:Wine / Q000032:analysis*
D003373:Coumaric Acids; D011422:Propionates; C495469:p-coumaric acid