Inhibition effect of poly(γ-glutamic acid) on lead-induced toxicity in mice.
The objectives of this study were to evaluate the efficiency in treatment of lead-induced intoxication in mice with γ-PGA as chelating agent and compare with the drug (meso-2,3-dimercaptosuccinic acid). The results showed the incorporation of γ-PGA at 200 and 400 mg/kg could reduce the accumulation of lead in the liver, heart, and testis; however, the latter was more effective in decreasing the lead content in the kidney and spleen. Nevertheless, both doses failed to inhibit the lead accumulation in the lung and brain. Additionally, both doses of γ-PGA could reduce TBARs in the kidney and brain, as well as elevate δ-aminolevulinic acid dehydrase (δ-ALAD) activity in blood and decrease glutamic pyruvic transaminase (GPT) and lactic dehydrogenase (LDH) activities in the serum. For hematological parameters, both white blood cells (WBCs) and hematocrite (HCT) were raised by 400 mg/kg of γ-PGA, while for both doses of γ-PGA, a slight decline in hemoglobin (HGB), mean cell volume (MCV), mean cell hemoglobin (MCH), and mean cell hemoglobin concentration (MCHC) was observed, with the red blood cells (RBCs) being unaffected.
Department of Emergency Medicine, Shin Kong Hospital, Taipei, Taiwan.
T L
TL
Wang
T H
TH
Kao
B Stephen
BS
Inbaraj
Y T
YT
Su
B H
BH
Chen
D000818:Animals; D002614:Chelating Agents / Q000008:administration & dosage*; D006454:Hemoglobins / Q000378:metabolism; D006801:Humans; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D007854:Lead / Q000378:metabolism / Q000633:toxicity*; D007855:Lead Poisoning / Q000188:drug therapy* / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011099:Polyglutamic Acid / Q000008:administration & dosage / Q000031:analogs & derivatives*
D002614:Chelating Agents; D006454:Hemoglobins; C511775:poly(gamma-glutamic acid); D011099:Polyglutamic Acid; D007854:Lead