Preparation of tea catechins using polyamide.
An adsorption separation method using Polyamide-6 (PA) as an adsorbent was developed to separate catechins from green tea extract. The adsorption capacity of total catechins for PA was 193.128 mg g⁻¹ with an adsorption selectivity coefficient K(A)(B) of total catechins over caffeine 21.717, which was better than macroporous resin model HPD 600. The Langmuir model and the pseudo-second order mode were primely fitted to describe its equilibrium data and adsorption kinetics, respectively. PA column separation by two-step elution using water and 80% (v/v) aqueous ethanol was established to prepare catechins complex which contained 670.808 mg g⁻¹ total catechins and 1.828 mg g⁻¹ caffeine. It is considered that PA was a promising adsorbent for selective isolation of catechins.
Zhejiang University Tea Research Institute, 268 Kaixuan Road, Hangzhou 310029, China.
Jian-Hui
JH
Ye
Liu-Xiang
LX
Wang
Hao
H
Chen
Jun-Jie
JJ
Dong
Jian-Liang
JL
Lu
Xin-Qiang
XQ
Zheng
Ming-Yan
MY
Wu
Yue-Rong
YR
Liang
D000327:Adsorption; D002110:Caffeine / Q000302:isolation & purification; D002209:Caprolactam / Q000031:analogs & derivatives* / Q000737:chemistry; D002392:Catechin / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D008956:Models, Chemical; D011108:Polymers / Q000737:chemistry*; D013662:Tea / Q000737:chemistry*
D011108:Polymers; D013662:Tea; C009916:nylon 6; D002110:Caffeine; D002209:Caprolactam; D002392:Catechin