Genetic characterization of the Korean LATC06 rinderpest vaccine strain.
We sequenced the genome of LATC06 generated by in vitro passage in Vero cells of the lapinized-avianized (LA) strain and compared its sequence to those of other rinderpest viruses. The LATC06 genome consists of 15882 nucleotides. Its transcriptional regulatory control sequences (TRSs) at gene boundaries are identical to those of the Kabete O strain. Cleavage sites for generating F1/F2 proteins were identified in the same amino acid position (aa 108) as F proteins in LATC06, L13, RBT1, Kabete O, and RBOK strains. There are three predicted N-glycosylation sites of H proteins in LA (Japan) and LATC06 strains. The six epitopes of H protein in the LA (Japan) strain that elicit immunodominant humoral responses are also found in the LATC06 strain.
Division of Veterinary Biotechnology, National Veterinary Research and Quarantine Service, Kyunggi-do, Anyang, Korea.
Jung-Yong
JY
Yeh
Chang-Hee
CH
Kwoen
Wooseog
W
Jeong
Hye-Young
HY
Jeoung
Hee Soo
HS
Lee
Dong-Jun
DJ
An
D000818:Animals; D002522:Chlorocebus aethiops; D000939:Epitopes / Q000235:genetics; D016679:Genome, Viral; D006023:Glycoproteins / Q000235:genetics; D006389:Hemagglutinins, Viral / Q000235:genetics; D008565:Membrane Proteins; D019590:Nucleocapsid Proteins / Q000235:genetics; D010802:Phylogeny; D019704:Protein Disulfide-Isomerases; D012367:RNA, Viral / Q000235:genetics; D012301:Rinderpest / Q000821:virology; D012302:Rinderpest virus / Q000235:genetics*; D017423:Sequence Analysis, RNA; D017386:Sequence Homology, Amino Acid; D014709:Vero Cells; D014760:Viral Fusion Proteins / Q000235:genetics
D000939:Epitopes; C079748:F protein, Rinderpest virus; D006023:Glycoproteins; D006389:Hemagglutinins, Viral; D008565:Membrane Proteins; D019590:Nucleocapsid Proteins; D012367:RNA, Viral; D014760:Viral Fusion Proteins; C079749:hemagglutinin protein, Rinderpest virus; C481383:nucleocapsid protein, Rinderpest virus; D019704:Protein Disulfide-Isomerases