Photoelectrochemical detection of pentachlorophenol with a multiple hybrid CdSe(x)Te(1-x)/TiO2 nanotube structure-based label-free immunosensor.
Driven by the urgent demand of detecting trace amounts of pentachlorophenol (PCP) in contaminative water, a label-free immunosensor with ultra sensitivity and high selectivity was constructed based on a hybrid CdSe(x)Te(1-x) (0 ≤ x ≤ 1) nanocrystal (NCs)-modified TiO(2) nanotube (NT) arrays for the first time. The CdSe(x)Te(1-x) NCs were photoelectrodeposited on inner and outer space of the TiO(2) NTs, leading to high photoelectrical conversion efficiency in the visible region. PCP antibodies are covalently conjugated on the TiO(2) NTs due to the large surface area and good biocompatibility. Since the photocurrent is highly dependent on the TiO(2) surface properties, the specific interaction between PCP and the antibodies results in a sensitive change in the photocurrent, with a limit of detection (LOD) of 1 pM. High sensor-to-sensor reproducibility is achieved. The sensor was applied for the direct analysis of river water samples.
State Key Laboratory of Chemo/Biosensing and Chemometrics, Department of Chemistry, Hunan University, Changsha 410082, P. R. China.
Qing
Q
Kang
Lixia
L
Yang
Yufang
Y
Chen
Shenglian
S
Luo
Lingfei
L
Wen
Qingyun
Q
Cai
Shouzhuo
S
Yao
D055910:Antibodies, Immobilized / Q000276:immunology; D015374:Biosensing Techniques / Q000379:methods*; D019187:Cadmium Compounds / Q000737:chemistry; D055664:Electrochemical Techniques / Q000379:methods*; D007118:Immunoassay / Q000379:methods*; D043942:Nanotubes / Q000737:chemistry*; D010416:Pentachlorophenol / Q000032:analysis*; D018036:Selenium Compounds / Q000737:chemistry; D013691:Tellurium / Q000737:chemistry; D014025:Titanium / Q000737:chemistry; D014874:Water Pollutants, Chemical / Q000032:analysis*
D055910:Antibodies, Immobilized; D019187:Cadmium Compounds; D018036:Selenium Compounds; D014874:Water Pollutants, Chemical; C009495:titanium dioxide; C058667:cadmium selenide; D014025:Titanium; D010416:Pentachlorophenol; D013691:Tellurium; C028337:cadmium telluride