Redox and electrocatalytic properties of mimochrome VI, a synthetic heme peptide adsorbed on gold.
Mimochrome VI (MC-VI) is a synthetic heme peptide containing a helix-heme-helix sandwich motif designed to reproduce the catalytic activity of heme oxidases. The thermodynamics of Fe(III) to Fe(II) reduction and the kinetics of the electron-transfer process for MC-VI immobilized through hydrophobic interactions on a gold electrode coated with a nonpolar SAM of decane-1-thiol have been determined through cyclic voltammetry. Immobilization slightly affects the reduction potential of MC-VI, which under these conditions electrocatalytically turns over molecular oxygen. This work sets the premise for the exploitation of totally synthetic mimochrome-modified electrode surfaces for clinical and pharmaceutical biosensing.
Department of Chemistry, University of Modena and Reggio Emilia, via Campi 183, 41125 Modena, Italy.
Antonio
A
Ranieri
Stefano
S
Monari
Marco
M
Sola
Marco
M
Borsari
Gianantonio
G
Battistuzzi
Paola
P
Ringhieri
Flavia
F
Nastri
Vincenzo
V
Pavone
Angelina
A
Lombardi
D000327:Adsorption; D000595:Amino Acid Sequence; D002384:Catalysis; D045304:Cytochromes c / Q000737:chemistry; D003905:Deuteroporphyrins / Q000737:chemistry*; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D006046:Gold / Q000737:chemistry*; D006418:Heme / Q000737:chemistry*; D008667:Metalloproteins / Q000737:chemistry*; D008968:Molecular Conformation; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010100:Oxygen / Q000737:chemistry; D010455:Peptides / Q000737:chemistry*; D011487:Protein Conformation
C501456:Cobalt(III)-mimochrome IV; D003905:Deuteroporphyrins; D008667:Metalloproteins; D010455:Peptides; D006418:Heme; D006046:Gold; D045304:Cytochromes c; D010100:Oxygen