Single-strand DNA detection using a planar photonic-crystal-waveguide-based sensor.
We report an experimental demonstration of single-strand DNA (ssDNA) detection at room temperature using a photonic-crystal-waveguide-based optical sensor. The sensor surface was previously biofunctionalized with ssDNA probes to be used as specific target receptors. Our experiments showed that it is possible to detect these hybridization events using planar photonic-crystal structures, reaching an estimated detection limit as low as 19.8 nM for the detection of the complementary DNA strand.
Nanophotonics Technology Center, Universidad Politécnica de Valencia, Camino de Vera s/n, 46022 Valencia, Spain. vertocca@ntc.upv.es
V
V
Toccafondo
J
J
García-Rupérez
M J
MJ
Bañuls
A
A
Griol
J G
JG
Castelló
S
S
Peransi-Llopis
A
A
Maquieira
D004277:DNA, Single-Stranded / Q000032:analysis* / Q000737:chemistry; D057230:Limit of Detection; D008855:Microscopy, Electron, Scanning; D009693:Nucleic Acid Hybridization; D055096:Optical Devices; D017785:Photons
D004277:DNA, Single-Stranded