Characterization of cellular internalization pathways for CPP-mediated oligonucleotide delivery.
The methods for evaluating internalization pathways of cellular CPP-mediated ON delivery utilizing a pre-mRNA splice correction assay and fluorescence-based quantification are described. Examples for characterization of CPP uptake routes, employing various endocytosis inhibitors, and special treatment conditions are demonstrated. The methods are developed to characterize cellular delivery of pre-mRNA splice switching peptide nucleic acids conjugated to CPPs by disulfide bond.
Department of Neurochemistry, Stockholm University, Stockholm, Sweden.
Peter
P
Guterstam
Samir E L
SE
Andaloussi
Ulo
U
Langel
D000595:Amino Acid Sequence; D001483:Base Sequence; D057846:Cell-Penetrating Peptides / Q000737:chemistry / Q000378:metabolism*; D004220:Disulfides / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry / Q000378:metabolism*; D004705:Endocytosis / Q000187:drug effects; D005470:Fluorometry; D006367:HeLa Cells; D006801:Humans; D008156:Luciferases / Q000235:genetics; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020135:Peptide Nucleic Acids / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D021381:Protein Transport; D012326:RNA Splicing; D014162:Transfection
D057846:Cell-Penetrating Peptides; D004220:Disulfides; D004337:Drug Carriers; D009841:Oligonucleotides; D020135:Peptide Nucleic Acids; D008156:Luciferases