[Determination of tenacissoside H in Marsdeniae tenacissimae by HPLC-ELSD].
In order to improve the quality standard of Marsdenia tenacissima, a quantitative determination method of tenacissoside H was developed using high performance liquid chromatography. The method was carried out on a YMC ODS-H80 (4.6 mm x 250 mm, 4 microm) column eluted with a mixture of acetonitrile and water (50:50) as the mobile phase. The flow rate was 0.8 mL x min(-1) and the column temperature was 35 degrees C. An evaporative light scattering detector (ELSD) was used with the temperature of drift tube set at 60 degrees C and the gas flow rate of nitrogen set at 1.5 mL x min(-1). The calibration curve was linear in the range from 0.5625 to 36.00 microg (r = 0.9998). The average recovery and RSD were 99.41% and 1.8%, respectively. The contents of tenacissoside H in the 11 samples from different habitats varied from 0.201% to 0.862%. The method established in this paper is specific and reliable to control and evaluate the quality of M. tenacissima.
Department of Pharmacognosy, School of Pharmacy, Fudan University, Shanghai 201203, China.
Huali
H
Li
Yan
Y
Lu
Jiayun
J
Hu
Daofeng
D
Chen
D002851:Chromatography, High Pressure Liquid; D006027:Glycosides / Q000032:analysis*; D031101:Marsdenia / Q000737:chemistry*; D015203:Reproducibility of Results
D006027:Glycosides