Detection of adrenaline on poly(3-aminobenzylamine) ultrathin film by electrochemical-surface plasmon resonance spectroscopy.
In this Article, we present a novel method to detect adrenaline on poly(3-aminobenzylamine) (PABA) ultrathin films by electrochemical-surface plasmon resonance (EC-SPR) spectroscopy. We prepared a PABA film, which specifically reacts with adrenaline, on a gold electrode by electropolymerization of 3-aminobenzylamine. The specific reaction of benzylamine within the PABA structure with adrenaline was studied by XPS, UV-vis spectroscopy, and EC-SPR techniques. Adrenaline was detected in real time by EC-SPR spectroscopy, which provides simultaneous monitoring of both optical SPR reflectivity and electrochemical current responses upon injecting adrenaline into the PABA thin film. The number of changes in both current and SPR reflectivity on the injection of adrenaline exhibited the linear relation to the concentration, and the detection limit was 100 pM. The responses were distinctive to those for uric acid and ascorbic acid, which are major interferences of adrenaline.
Center for Transdisciplinary Research and Graduate School of Science and Technology, Niigata University, 8050 Ikarashi 2-nocho, Nishi-ku, Niigata 950-2181, Japan. ababa@eng.niigata-u.ac.jp
Akira
A
Baba
Touru
T
Mannen
Yasuo
Y
Ohdaira
Kazunari
K
Shinbo
Keizo
K
Kato
Futao
F
Kaneko
Nobuko
N
Fukuda
Hirobumi
H
Ushijima
D010129:4-Aminobenzoic Acid / Q000737:chemistry; D000327:Adsorption; D000818:Animals; D001205:Ascorbic Acid / Q000737:chemistry; D002623:Chemistry Techniques, Analytical; D004563:Electrochemistry / Q000379:methods*; D004837:Epinephrine / Q000032:analysis* / Q000737:chemistry*; D006801:Humans; D056951:Photoelectron Spectroscopy / Q000379:methods; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D020349:Surface Plasmon Resonance / Q000379:methods*; D014466:Ultraviolet Rays
D001205:Ascorbic Acid; D010129:4-Aminobenzoic Acid; D004837:Epinephrine