Electrical and optical spectroscopy for quantitative screening of hepatic steatosis in donor livers.
Macro-steatosis in deceased donor livers is increasingly prevalent and is associated with poor or non-function of the liver upon reperfusion. Current assessment of the extent of steatosis depends upon the macroscopic assessment of the liver by the surgeon and histological examination, if available. In this paper we demonstrate electrical and optical spectroscopy techniques which quantitatively characterize fatty infiltration in liver tissue. Optical spectroscopy showed a correlation coefficient of 0.85 in humans when referenced to clinical hematoxylin and eosin (H&E) sections in 20 human samples. With further development, an optical probe may provide a comprehensive measure of steatosis across the liver at the time of procurement.
Engineering Department, University of Cambridge, 9 JJ Thomson Avenue, Cambridge CB3 OFA, UK. mclaugb@gmail.com
B L
BL
McLaughlin
A C
AC
Wells
S
S
Virtue
A
A
Vidal-Puig
T D
TD
Wilkinson
C J E
CJ
Watson
P A
PA
Robertson
D000818:Animals; D058266:Dielectric Spectroscopy / Q000295:instrumentation*; D005234:Fatty Liver / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D005260:Female; D006801:Humans; D008099:Liver / Q000378:metabolism / Q000473:pathology; D051379:Mice; D055100:Optical Fibers; D055620:Optical Phenomena; D019095:Point-of-Care Systems; D013055:Spectrophotometry, Infrared / Q000295:instrumentation*; D013997:Time Factors; D014019:Tissue Donors