Effects of sub-sonic vibration on the proliferation and maturation of 3T3-L1 cells.
OBJECTIVE
Although low and high intensity sub-sonic vibrations (SSV) have been shown to facilitate wound healing, very few studies have investigated the effects of SSV on 3T3-L1 preadipocytes. Therefore, the present study was undertaken to investigate the influence of SSV on the proliferation and maturation of 3T3-L1 preadipocytes.


METHODS
To evaluate the effect of SSV on 3T3-L1 cell proliferation, the cells were maintained in an apparatus that administered SSV (0.5 V) for 3 days at a frequency of 10, 20, 30, or 40 Hz. In addition, to study the effect of SSV on 3T3-L1 cell maturation, the cells were stimulated with SSV for 6 days at a frequency of 10, 20, 30, or 45 Hz.


RESULTS
Sub-sonic vibrations inhibited the proliferation of 3T3-L1 preadipocytes at frequencies of 20 and 30 Hz. Triglyceride levels in cells subjected to SSV at frequencies ranging from 10 to 30 Hz increased compared with those measured in control cells. The expression of adipogenic genes, such as PPAR-γ and C/EBP-α, markedly increased in response to SSV at 20 Hz and 30 Hz during maturation.


CONCLUSIONS
These results suggest that SSV affected adipogenic gene expression at 20 and 30 Hz.
Department of Medical Biotechnology, Dongguk University, Seoul 100-715, South Korea.
Eun-Seon
ES
Oh
Young-Kwon
YK
Seo
Hee-Hoon
HH
Yoon
Hyunjin
H
Cho
Moon-Young
MY
Yoon
Jung-Keug
JK
Park
D041721:3T3-L1 Cells / Q000737:chemistry / Q000166:cytology*; D000818:Animals; D001973:Bromodeoxyuridine; D049109:Cell Proliferation; D017931:DNA Primers / Q000235:genetics; D005786:Gene Expression Regulation / Q000502:physiology*; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D051379:Mice; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013778:Tetrazolium Salts; D013844:Thiazoles; D014280:Triglycerides / Q000032:analysis; D014732:Vibration / Q000627:therapeutic use*
D017931:DNA Primers; D013778:Tetrazolium Salts; D013844:Thiazoles; D014280:Triglycerides; D007770:L-Lactate Dehydrogenase; C022616:thiazolyl blue; D001973:Bromodeoxyuridine