Identification of Yersinia enterocolitica using a random genomic DNA microarray chip.
OBJECTIVE
To fabricate a DNA chip containing random fragments of genomic DNA of Yersinia enterocolitica and to verify its diagnostic ability.


RESULTS
A DNA microarray chip was fabricated using randomly fragmented DNA of Y. enterocolitica. Chips were hybridized with genomic DNA extracted from other Y. enterocolitica strains, other Yersinia spp. and bacteria in different genera. Genomic DNA extracted from Y. enterocolitica showed a significantly higher hybridization rate compared with DNA of other Yersinia spp. or bacterial genera, thereby distinguishing it from other bacteria.


CONCLUSIONS
A DNA chip containing randomly fragmented genomic DNA from Y. enterocolitica can detect Y. enterocolitica and clearly distinguish it from other Yersinia spp. and bacteria in different genera.


CONCLUSIONS
A microarray chip containing randomly fragmented genomic DNA of Y. enterocolitica was fabricated without sequence information, and its diagnostic ability to identify Y. enterocolitica was verified.
Graduate School of Life Sciences and Biotechnology, Korea University, Seoul, Korea.
J
J
Bang
L R
LR
Beuchat
M B
MB
Gu
H-I
HI
Chang
J-H
JH
Ryu
D016000:Cluster Analysis; D015342:DNA Probes / Q000235:genetics; D004269:DNA, Bacterial / Q000032:analysis; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D025341:Principal Component Analysis; D013045:Species Specificity; D015008:Yersinia enterocolitica / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification
D015342:DNA Probes; D004269:DNA, Bacterial