Development and strategies of cell-culture technology for influenza vaccine.
Influenza is a pandemic contagious disease and causes human deaths and huge economic destruction of poultry in the world. In order to control and prevent influenza, mainly type A, influenza vaccine for human and poultry were available since the 1940s and 1920s, respectively. In the development of vaccine production, influenza viruses were cultured originally from chicken embryos to anchorage-dependent cell lines, such as MDCK and Vero. The anchorage-independent lines have also been used to produce influenza virus, such as PER.C6 and engineering modified MDCK and Vero. During the process of influenza vaccine production, the common problem faced by all producers is how to improve the titer of influenza virus. This paper focuses on the developments of cell culture for influenza virus vaccine production, limitations of cell culture, and relative strategies for improvement virus yields in cell-culture systems.
Laboratory of Avian Medicine, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
Shao-Zhen
SZ
Feng
Pei-Rong
PR
Jiao
Wen-Bao
WB
Qi
Hui-Ying
HY
Fan
Ming
M
Liao
D000818:Animals; D001709:Biotechnology / Q000379:methods*; D018929:Cell Culture Techniques / Q000379:methods; D002460:Cell Line; D006801:Humans; D007252:Influenza Vaccines / Q000302:isolation & purification*; D009975:Orthomyxoviridae / Q000254:growth & development*; D013678:Technology, Pharmaceutical / Q000379:methods*
D007252:Influenza Vaccines