Synthesis of ethyl ferulate in organic medium using celite-immobilized lipase.
In the present work we have evaluated synthesis of ethyl ferulate by the esterification reaction of ferulic acid and ethanol catalyzed by a commercial lipase (Steapsin) immobilized onto celite-545 in a short period of 6h in DMSO. The immobilized lipase was treated with cross-linking agent glutaraldehyde (1%; v/v). The optimum synthesis of ethyl ferulate was recorded at 45°C, pH 8.5 and 1:1 ratio of ethanol and ferulic acid. Co(2+), Ba(2+)and Pb(2+) ions enhanced the synthesis of ethyl ferulate Hg(2+), Cd(3+)and NH(4+) ions had mild inhibitory effect. The celite-bound lipase produced 68 mM of ethyl ferulate under optimized reaction conditions.
Department of Biotechnology, Himachal Pradesh University, Shimla, India.
Ashok
A
Kumar
Shamsher S
SS
Kanwar
D002109:Caffeic Acids / Q000138:chemical synthesis*; D003373:Coumaric Acids / Q000737:chemistry*; D007692:Diatomaceous Earth / Q000737:chemistry*; D004789:Enzyme Activation; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry; D004951:Esterification; D000431:Ethanol / Q000737:chemistry*; D008049:Lipase / Q000737:chemistry*
D002109:Caffeic Acids; D003373:Coumaric Acids; D004800:Enzymes, Immobilized; D000431:Ethanol; C099085:ethyl ferulate; D007692:Diatomaceous Earth; C004999:ferulic acid; D008049:Lipase