[Irritant stability of raphides and tubers from Pinellia ternate].
OBJECTIVE
To investigate the irritation stability of raphides from Pinellia ternata and the contribution of raphides proteins on irritation.


METHODS
The irritation of raphides and tubers from P. ternata treated with different solvents or protease digestion were evaluated by the Draize test. The shape and appearance of raphides treated with immersion in different solvents were showed by scanning electron microscopy, and protein bands from raphides before and after protease digestion were showed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE).


RESULTS
The raphides gradually lost the irritation when immersed in methanol and ethanol, while scanning electron micrograph showed the fragility of the methanol and ethanol treated raphides. The crude tubers of P. ternata immersed in 75% solution of ethanol also lost the acridity. When treated with protease digestion, raphides lost the irritation as well as the many protein bands on the SDS-PAGE gel gradually disappeared.


CONCLUSIONS
Protein of the raphides could be involved in the raphides irritation.
College of pharmacy, Nanjing University of TCM, Nanjing 210029, China.
Hong-Li
HL
Yu
Hao
H
Wu
Xing-De
XD
Zhang
Fu-Yan
FY
Liu
D000818:Animals; D003228:Conjunctiva / Q000187:drug effects; D003460:Crystallization; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000633:toxicity*; D000431:Ethanol / Q000737:chemistry; D005128:Eye Diseases / Q000139:chemically induced*; D005260:Female; D008297:Male; D000432:Methanol / Q000737:chemistry; D030021:Pinellia / Q000737:chemistry; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000633:toxicity*; D035281:Plant Tubers / Q000737:chemistry; D011208:Powders; D011817:Rabbits; D013678:Technology, Pharmaceutical / Q000379:methods*
D004365:Drugs, Chinese Herbal; D010940:Plant Proteins; D011208:Powders; D000431:Ethanol; D000432:Methanol