[Determination of aurantio-obtusin and chrysophanol in cassiae semen by HPLC].
OBJECTIVE
To improve the determination method of Cassiae Semen in China Pharmacopeia edition 2005. MMETHOD: Aurantio-obtusin and chrysophanol were separated on an Inertsil ODS-3 column with acetonitrile-0.1% phosphoric acid solutiona as mobile phase in gradient elution.


RESULTS
The linear ranges of aurantio-obtusin and chrysophanol were 2.3-230, 2.87-286 mg x L(-1), respectively. The average recover were 100.3% (RSD 3.1%), and 99.5% (RSD 2.5%) respectively.


CONCLUSIONS
This method is specific, accurate and reproducible, and can be used for the assay of Cassiae Semen in the new edition of Chinese pharmacopoeia.
Chongqing Academy of Chinese Materia Medica, Chongqing 400065, China.
Xiaoping
X
Huang
Guoyue
G
Zhong
Xuemei
X
Zhang
Yi
Y
Zhang
D000880:Anthraquinones / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D032904:Cinnamomum aromaticum / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000737:chemistry; D015203:Reproducibility of Results
D000880:Anthraquinones; D004365:Drugs, Chinese Herbal; C027113:chrysophanic acid