Receptor for activated C kinase 1 stimulates nascent polypeptide-dependent translation arrest.
Nascent peptide-dependent translation arrest is crucial for the quality control of eukaryotic gene expression. Here we show that the receptor for activated C kinase 1 (RACK1) participates in nascent peptide-dependent translation arrest, and that its binding to the 40S subunit is crucial for this. Translation arrest by a nascent peptide results in Dom34/Hbs1-independent endonucleolytic cleavage of mRNA, and this is stimulated by RACK1. We propose that RACK1 stimulates the translation arrest that is induced by basic amino-acid sequences that leads to endonucleolytic cleavage of the mRNA, as well as to co-translational protein degradation.
Nagoya University, Chikusa-ku, Japan.
Kazushige
K
Kuroha
Mayuko
M
Akamatsu
Lyudmila
L
Dimitrova
Takehiko
T
Ito
Yuki
Y
Kato
Katsuhiko
K
Shirahige
Toshifumi
T
Inada
D048868:Adaptor Proteins, Signal Transducing / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D001483:Base Sequence; D019204:GTP-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D008969:Molecular Sequence Data; D010455:Peptides / Q000378:metabolism*; D011485:Protein Binding; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D054657:Ribosome Subunits / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
C111825:ASC1 protein, S cerevisiae; D048868:Adaptor Proteins, Signal Transducing; D010455:Peptides; D012333:RNA, Messenger; D029701:Saccharomyces cerevisiae Proteins; D019204:GTP-Binding Proteins