Electrostatic interaction and complex formation between gum arabic and bovine serum albumin.
The interaction of gum arabic (GA) and bovine serum albumin (BSA) has been investigated through turbidity and light scattering intensity measurements and by the use of dynamic light scattering, laser Doppler velocimetry, and isothermal titration calorimetry. It has been shown that GA and BSA can form soluble and insoluble complexes depending on the solution pH and the mixing ratio and is a function of the net charge on the complex. Soluble complexes were obtained when the electrophoretic mobility was greater than ±1. 5 μm s(-1) V(-1) cm(-1). Changes in the value of the isoelectric point of the complexes with mixing ratio and isothermal titration calorimetric data indicated that complexes formed at pHs 3 and 4 consisted of ∼60 BSA molecules for every GA molecule, while at pH 5 there were ∼10 BSA molecules per GA molecule. Calorimetric studies also indicated that the interaction occurred in two stages at both pH 3 and pH 4, but that the nature of the interaction at these two pH values was significantly different. This was attributed to differences in the relative magnitude of the positive and negative charges on the BSA and GA, respectively, and possibly due to changes in the BSA conformation. The fact that there is an interaction at pH 5, which is above the isoelectric point of the BSA, is due to the interaction of the carboxylate groups on the GA with positive patches on the BSA or to the charge regulation of the protein-polysaccharide system brought about by changes in dissociation equilibria. Complexation is reduced as the ionic strength of the solvent increases and is prevented at a NaCl concentration of 120 mM.
Centre for Water-Soluble Polymers, Glyndwr University, Plas Coch, Mold Road, Wrexham, LL11 2AW, United Kingdom.
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Vinayahan
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Williams
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Phillips
D006170:Gum Arabic / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D009391:Nephelometry and Turbidimetry; D009994:Osmolar Concentration; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D012995:Solubility; D055672:Static Electricity
D012710:Serum Albumin, Bovine; D006170:Gum Arabic