Regulation of longevity by genes required for the functions of AIY interneuron in nematode Caenorhabditis elegans.
In Caenorhabditis elegans, functional ttx-3, sra-11, ceh-10, and ceh-23 genes are required for the functions of AIY interneuron. Compared to wild-type N2, mutations in ttx-3 and ceh-10 significantly decreased lifespan, whereas mutations in sra-11 and ceh-23 did not obviously influence nematode lifespan. Mutations in ttx-3 and ceh-10 were associated closely with lower pumping rates at adult day 8 and caused a more rapid accumulated intestinal autofluorescence than wild-type N2. Mutations in ceh-10 remarkably affected fertility and egg number in the uterus. The regulation of ttx-3 and ceh-10 on longevity was not temperature-dependent, and ttx-3, and ceh-10 mutants all formed very few dauers at 27°C. The shortened lifespan of the ttx-3 or ceh-10 mutants was completely or largely rescued by expression of TTX-3 or CEH-10 in AIY interneurons. Moreover, the long-lived phenotype of the daf-2 mutant could be suppressed by both the ttx-3 and the ceh-10 mutations. Furthermore, ablation of AIY interneurons shortened the longevity of wild-type and the daf-2 mutant. Therefore, ttx-3 and ceh-10 regulate the longevity through influencing the insulin/IGF signaling pathway in C. elegans.
Institute of Life Sciences, Southeast University, Nanjing 210009, China.
Lulu
L
Shen
Yaou
Y
Hu
Ting
T
Cai
Xingfeng
X
Lin
Dayong
D
Wang
D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics / Q000502:physiology*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000502:physiology*; D015870:Gene Expression; D018398:Homeodomain Proteins / Q000235:genetics / Q000502:physiology; D007328:Insulin / Q000378:metabolism; D007395:Interneurons / Q000502:physiology*; D007422:Intestines / Q000502:physiology; D008136:Longevity / Q000235:genetics*; D009154:Mutation; D009479:Neuropeptides / Q000235:genetics / Q000502:physiology; D010614:Pharynx / Q000502:physiology; D011972:Receptor, Insulin / Q000235:genetics / Q000502:physiology; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000502:physiology
D029742:Caenorhabditis elegans Proteins; D018398:Homeodomain Proteins; D007328:Insulin; D009479:Neuropeptides; D043562:Receptors, G-Protein-Coupled; C502934:SRA-11 protein, C elegans; C108368:TTX-3 protein, C elegans; C094292:ceh-10 protein, C elegans; C107483:DAF-2 protein, C elegans; D011972:Receptor, Insulin