Genome sequence of Kitasatospora setae NBRC 14216T: an evolutionary snapshot of the family Streptomycetaceae.
Kitasatospora setae NBRC 14216(T) (=KM-6054(T)) is known to produce setamycin (bafilomycin B1) possessing antitrichomonal activity. The genus Kitasatospora is morphologically similar to the genus Streptomyces, although they are distinguishable from each other on the basis of cell wall composition and the 16S rDNA sequence. We have determined the complete genome sequence of K. setae NBRC 14216(T) as the first Streptomycetaceae genome other than Streptomyces. The genome is a single linear chromosome of 8,783,278 bp with terminal inverted repeats of 127,148 bp, predicted to encode 7569 protein-coding genes, 9 rRNA operons, 1 tmRNA and 74 tRNA genes. Although these features resemble those of Streptomyces, genome-wide comparison of orthologous genes between K. setae and Streptomyces revealed smaller extent of synteny. Multilocus phylogenetic analysis based on amino acid sequences unequivocally placed K. setae outside the Streptomyces genus. Although many of the genes related to morphological differentiation identified in Streptomyces were highly conserved in K. setae, there were some differences such as the apparent absence of the AmfS (SapB) class of surfactant protein and differences in the copy number and variation of paralogous components involved in cell wall synthesis.
NITE Bioresource Information Center, Department of Biotechnology, National Institute of Technology and Evaluation, 2-49-10 Nishihara, Shibuya-ku, Tokyo 151-0066, Japan.
Natsuko
N
Ichikawa
Akio
A
Oguchi
Haruo
H
Ikeda
Jun
J
Ishikawa
Shigeru
S
Kitani
Yumi
Y
Watanabe
Sanae
S
Nakamura
Yoko
Y
Katano
Emi
E
Kishi
Machi
M
Sasagawa
Akiho
A
Ankai
Shigehiro
S
Fukui
Yoshimi
Y
Hashimoto
Sachi
S
Kamata
Misa
M
Otoguro
Satoshi
S
Tanikawa
Takuya
T
Nihira
Sueharu
S
Horinouchi
Yasuo
Y
Ohnishi
Masayuki
M
Hayakawa
Tomohisa
T
Kuzuyama
Akira
A
Arisawa
Fumiki
F
Nomoto
Hiromi
H
Miura
Yoko
Y
Takahashi
Nobuyuki
N
Fujita
D000595:Amino Acid Sequence; D000994:Antitrichomonal Agents / Q000378:metabolism; D001483:Base Sequence; D002876:Chromosomes, Bacterial / Q000235:genetics; D004269:DNA, Bacterial / Q000032:analysis; D019143:Evolution, Molecular; D018507:Gene Expression Regulation, Developmental; D016680:Genome, Bacterial; D018942:Macrolides / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D012329:RNA, Bacterial / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D012343:RNA, Transfer / Q000235:genetics; D013306:Streptomycetaceae / Q000145:classification* / Q000235:genetics*
D000994:Antitrichomonal Agents; D004269:DNA, Bacterial; D018942:Macrolides; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; C032953:setamycin; D012343:RNA, Transfer