[The neuroglobin expression when retinal ganglion cells death in acute rats' retina ischemia].
OBJECTIVE
To investigate the intra-retinal expression of neuroglobin (Ngb) and death of retinal ganglion cells (RGCs) in acute retina ischemia rats.


METHODS
It was an experimental study. The acute retina ischemia model was established by specific hypothesised left retina artery of Sprague-Dawley rats. Forty rats were divided into four groups (0, 15, 30, 60 min) by the time of retina ischemia. Every group has 10 rats, in one group random 3 rats were detected by Western blotting; 4 rats were detected by ganglion cell counted by hematoxylin and eosin stain and immunohistochemistry fluorescence intensity analysis. The rest 3 rats were detected by Western blotting. The difference among different data were analyzed statistically by One-factor analysis of variance and LSD-t analysis.


RESULTS
The intra-retinal expression of Ngb reached maximum after acute ischemia 15 minute (P = 0.000). then the expression began decreasing. After 30 minute acute ischemia, the expression of Ngb had approached normal (P = 0.728), while, the cell number of RGCs began lower than 0 min group (P = 0.011); after 60 minute acute ischemia, the expression of Ngb had been obviously lower than 0 min group (P = 0.001), the cell number of RGCs had been further lower than 0 min group (P = 0.000). The expression of Ngb in RGCs layer was highest in rat retina. The expression in inner plexiform layer and external plexiform layer were lower than the former. The expression of Ngb RGCs was mostly intracytoplasm. After 30 minute acute ischemia, the expression of Ngb were detected in mitochondrial outer compartment and mitochondrial cristae, but in cytoplasm of inner nuclear layer and outer nuclear layer the Ngb was not found.


CONCLUSIONS
Ngb quickly steps-up when RGCs die in acute retina ischemia, and mainly expresses intracytoplasm of RGCs. It has tense relationships with nerve cells' survival in hypoxia.
Department of Ophthalmology, General Hospital of Beijing Command Region, Beijing, China.
Guo-peng
GP
Ding
Zhen-guo
ZG
Yan
Tao
T
He
Si-wei
SW
You
Yao-yu
YY
Li
Xue-feng
XF
Shen
Tao
T
Wang
Yu-kai
YK
Zhang
Xiao-feng
XF
Huang
D000818:Animals; D016923:Cell Death; D005260:Female; D005914:Globins / Q000378:metabolism*; D007511:Ischemia / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D000078787:Neuroglobin; D051381:Rats; D017207:Rats, Sprague-Dawley; D012165:Retinal Ganglion Cells / Q000166:cytology* / Q000473:pathology
D009419:Nerve Tissue Proteins; D000078787:Neuroglobin; D005914:Globins