The role of the interplay between polymer architecture and bacterial surface properties on the microbial adhesion to polyoxazoline-based ultrathin films.
Surface platforms were engineered from poly(L-lysine)-graft-poly(2-methyl-2-oxazoline) (PLL-g-PMOXA) copolymers to study the mechanisms involved in the non-specific adhesion of Escherichia coli (E. coli) bacteria. Copolymers with three different grafting densities α (PMOXA chains/Lysine residue of 0.09, 0.33 and 0.56) were synthesized and assembled on niobia (Nb₂O₅) surfaces. PLL-modified and bare niobia surfaces served as controls. To evaluate the impact of fimbriae expression on the bacterial adhesion, the surfaces were exposed to genetically engineered E. coli strains either lacking, or constitutively expressing type 1 fimbriae. The bacterial adhesion was strongly influenced by the presence of bacterial fimbriae. Non-fimbriated bacteria behaved like hard, charged particles whose adhesion was dependent on surface charge and ionic strength of the media. In contrast, bacteria expressing type 1 fimbriae adhered to the substrates independent of surface charge and ionic strength, and adhesion was mediated by non-specific van der Waals and hydrophobic interactions of the proteins at the fimbrial tip. Adsorbed polymer mass, average surface density of the PMOXA chains, and thickness of the copolymer films were quantified by optical waveguide lightmode spectroscopy (OWLS) and variable-angle spectroscopic ellipsometry (VASE), whereas the lateral homogeneity was probed by time-of-flight secondary ion mass spectrometry (ToF-SIMS). Streaming current measurements provided information on the charge formation of the polymer-coated and the bare niobia surfaces. The adhesion of both bacterial strains could be efficiently inhibited by the copolymer film only with a grafting density of 0.33 characterized by the highest PMOXA chain surface density and a surface potential close to zero.
BioInterfaceGroup, Laboratory for Surface Science and Technology, Department of Materials, ETH Zurich, CH-8093, Zurich, Switzerland.
Bidhari
B
Pidhatika
Jens
J
Möller
Edmondo M
EM
Benetti
Rupert
R
Konradi
Ekaterina
E
Rakhmatullina
Andreas
A
Mühlebach
Ralf
R
Zimmermann
Carsten
C
Werner
Viola
V
Vogel
Marcus
M
Textor
D001422:Bacterial Adhesion / Q000187:drug effects*; D018441:Biofilms / Q000187:drug effects*; D002686:Chitin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D015169:Colony Count, Microbial; D004926:Escherichia coli / Q000166:cytology / Q000187:drug effects* / Q000254:growth & development / Q000502:physiology*; D010861:Fimbriae, Bacterial / Q000187:drug effects; D008858:Microscopy, Phase-Contrast; D009556:Niobium / Q000494:pharmacology; D011073:Polyamines; D011107:Polylysine / Q000737:chemistry / Q000494:pharmacology*; D018629:Spectrometry, Mass, Secondary Ion; D055672:Static Electricity; D013499:Surface Properties / Q000187:drug effects
D011073:Polyamines; C117403:poly(2-methyl-2-oxazoline); D009556:Niobium; D002686:Chitin; D011107:Polylysine