[Identification of allele variants of cattle milk productivity genes using PCR anti-primer method].
In this work we have demonstrated two independent real-time PCR methods for detection single nucleotide polymorphism (SNP) of genes csn and acyl-CoA: diacylglycerol acyltransferase 1 (dgat) in cattle. We have analyzed 296 samples of milk production cattle of Ukrainian breeding. The genotype frequencies were AA--0.58, AB--0.34, BB--0.08 for csn gene and for dgat gene--AA--0.7, AK--0.26, KK--0.04. High efficiency of so called "anti-primer" method was shown. Duration of anti-primer PCR reaction was about 2-2.5 hours only and provided full investigation of unknown gene allele.
A N
AN
Rashydov
V H
VH
Spyrydonov
O M
OM
Konoval
M D
MD
Mel'nychuk
D000483:Alleles; D000818:Animals; D002364:Caseins / Q000235:genetics*; D002417:Cattle; D017931:DNA Primers; D003612:Dairying; D051048:Diacylglycerol O-Acyltransferase / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D007774:Lactation / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D014455:Ukraine
D002364:Caseins; D017931:DNA Primers; D051048:Diacylglycerol O-Acyltransferase