Methods for investigation of osteoclastogenesis using mouse embryonic stem cells.
Investigation of osteoclastogenesis in vivo, especially in early development, has proven difficult because of the accessibility of these early embryonic stages. Our ability to culture embryonic stem cells (ESCs) in vitro has overcome this difficulty as these versatile cells can be expanded endlessly. Thus, the whole process of osteoclastogenesis can be monitored in these cultures through the microscope and with the help of molecular biology techniques. We have developed two methods to induce osteoclasts, the bone matrix remodeling cells, from murine ESCs. Surprisingly, one of these induction methods produces osteoclasts, osteoblasts, and also endothelial cells in the same culture dish. Hence, it is likely that ESCs in culture mimic the in vivo development of osteoclasts.
Max Planck Institute for Infection Biology, Berlin, Germany. tsuneto@mpiib-berlin.mpg.de
Motokazu
M
Tsuneto
Toshiyuki
T
Yamane
Shin-Ichi
S
Hayashi
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D002460:Cell Line; D048430:Cell Shape; D018920:Coculture Techniques; D003114:Colony-Forming Units Assay; D053648:Cytokine Receptor Common beta Subunit / Q000378:metabolism; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism; D042783:Endothelial Cells / Q000166:cytology; D007150:Immunohistochemistry; D051379:Mice; D010006:Osteoblasts / Q000166:cytology; D010010:Osteoclasts / Q000166:cytology* / Q000378:metabolism; D010012:Osteogenesis; D013194:Staining and Labeling
D053648:Cytokine Receptor Common beta Subunit