[Analysis on genetic alterations of Lewis lung cancer by RAPD and cloning of tumor-differential DNA fragment].
BACKGROUND
To detect the genetic alterations and to find the tumor-related DNA fragments in Lewis lung cancer.


METHODS
Genomic DNAs from Lewis lung cancer and C57BL/ 6J mouse normal tissues were amplified by random amplified polymorphic DNA( RAPD) with 105 10-bp random primers. The RAPD bands showing obvious difference in tumor tissue DNA corresponding to that of normal tissuewere cloned and sequenced. DNA sequences were analyzed and compared with GenBank data.


RESULTS
There were 25 abnormal profiles out of the 105 primers generated polymorphic profile when the Lewis lung cancer RAPD profile was compared to that of its normal tissue DNA. The tumor-differential fragments L7-2 and L7-11 were obtained with primer AB7-2 and AB7-11 respectively. DNA sequence analysis revealed that L7-2 was a 730 bp fragment, and its sequence did not show significant homologies with known DNA sequences. L7-11 was a 779 bp fragment, and its sequence shared 90% homology with mouse V kappa 21-11 gene.


CONCLUSIONS
Genetic alterations in Lewis lung cancer genomic DNA have been detected by RAPD, and tumor-related DNA fragments L7-2 and L7-11 have been found in this study, which may be associated with tumorigenesis of Lewis lung cancer .
Institute of Pathology , Southwest Hospital, Third Military Medical University , Chongqing 400038, P, R, China.
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Li
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Chen
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Wei