Stimulatory effect of LPS and feedback effect of PGE2 on IL-27 production.
Interleukin (IL)-27 is a new member of the IL-6/IL-12 family, composed of two subunits, the Epstein-Barr virus-induced gene 3 (EBI3) and p28 chains (p28), and produced by activated monocytes and dendritic cells. IL-27 plays an important role in the regulation of differentiation of naive T helper cells and has diverse effects on innate immune cells. However, the pro-inflammatory mechanisms of IL-27 are still not well known. In this study, we investigated the effect of lipopolysaccharide (LPS) on the production of IL-27. We found that LPS-stimulated IL-27 production was in a dose-dependent and time-dependent manner in THP-1 cells. We have also shown that IL-27 induced PGE2 production and COX-2 gene expression at the level of mRNA as well as protein. Moreover, we found feed back effect of PGE2 on the production of IL-27 in THP-1 cells. The results suggest that PGE2 significantly inhibits LPS-induced IL-27 production, without affecting basal IL-27 expression. Further experiment suggests that PGE2 and LPS regulate IL-27 through NF-κB pathway. Our findings may have wide implication for IL-27 in inflammatory diseases.
Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, China.
C L
CL
Zhu
Y H
YH
Cao
R
R
Zhang
Y
Y
Song
W Y
WY
Liu
F
F
Pan
Y
Y
Li
Y
Y
Zhu
F
F
Liu
J G
JG
Wu
D002460:Cell Line; D051546:Cyclooxygenase 2 / Q000378:metabolism*; D015232:Dinoprostone / Q000378:metabolism*; D004306:Dose-Response Relationship, Immunologic; D025461:Feedback, Physiological; D006801:Humans; D020381:Interleukin-17 / Q000276:immunology / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000276:immunology*; D009000:Monocytes / Q000276:immunology
D020381:Interleukin-17; D008070:Lipopolysaccharides; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; D015232:Dinoprostone