Autophagy is induced by the type III secretion system of Vibrio alginolyticus in several mammalian cell lines.
Vibrio alginolyticus is a gram-negative bacterium and has been recognized as an opportunistic pathogen in marine animals as well as humans. Here, we further characterized a cell death mechanism caused by this bacterium in several mammalian cell lines. The T3SS of V. alginolyticus killed HeLa cells by a very similar cell cytolysis mechanism in fish cells, as evidenced by cell rounding and LDH release; however, DNA fragmentation was not observed. Further studies showed that caspase-1 and caspase-3 were not activated during the T3SS-mediated cell death, indicating that the death mechanism is completely independent of pyroptosis and apoptosis in HeLa cells. Conversely, autophagy was detected during the T3SS-mediated cell death by the appearance of MDC-labeled punctate fluorescence and accumulation of autophagic vesicles. Moreover, western blot analysis revealed increase in conversion of LC3-I to LC3-II in infected mammalian cell lines, confirming that autophagy occurs during the process. Together, these data demonstrate that the death process used by V. alginolyticus in mammalian cells is different from that in fish cells, including induction of autophagy, cell rounding and osmotic lysis. This study provides some evidences hinting that differences in death mechanism in responses to V. alginolyticus infection may be attributed to the species of infected cells from which it was derived.
Key Laboratory of Applied Marine Biology of Guangdong Province and Key Laboratory of Marine Bio-Resources Sustainable Utilization, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, People's Republic of China.
Zhe
Z
Zhao
Lvping
L
Zhang
Chunhua
C
Ren
Jingjing
J
Zhao
Chang
C
Chen
Xiao
X
Jiang
Peng
P
Luo
Chao-Qun
CQ
Hu
D000818:Animals; D017209:Apoptosis / Q000502:physiology; D001343:Autophagy / Q000502:physiology*; D016466:CHO Cells; D053148:Caspase 3 / Q000378:metabolism; D002460:Cell Line; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000473:pathology; D006224:Cricetinae; D003412:Cricetulus; D005399:Fishes; D006367:HeLa Cells; D006801:Humans; D013997:Time Factors; D014735:Vibrio Infections / Q000201:enzymology / Q000503:physiopathology*; D044149:Vibrio alginolyticus / Q000235:genetics / Q000378:metabolism*
D053148:Caspase 3