Heart wall velocimetry and exogenous contrast-based cardiac flow imaging in Drosophila melanogaster using Doppler optical coherence tomography.
Drosophila melanogaster (fruit fly) is a central organism in biology and is becoming increasingly important in the cardiovascular sciences. Prior work in optical imaging of the D. melanogaster heart has focused on static and dynamic structural anatomy. In the study, it is demonstrated that Doppler optical coherence tomography can quantify dynamic heart wall velocity and hemolymph flow in adult D. melanogaster. Since hemolymph is optically transparent, a novel exogenous contrast technique is demonstrated to increase the backscatter-based intracardiac Doppler flow signal. The results presented here open up new possibilities for functional cardiovascular phenotyping of normal and mutant D. melanogaster.
Children's Hospital Boston, and Harvard Medical School, Department of Pediatrics, Boston, Massachusetts 02115, USA. michael.choma@yale.edu
Michael A
MA
Choma
Melissa J
MJ
Suter
Benjamin J
BJ
Vakoc
Brett E
BE
Bouma
Guillermo J
GJ
Tearney
D000818:Animals; D001783:Blood Flow Velocity; D002320:Cardiovascular Physiological Phenomena; D003287:Contrast Media / Q000008:administration & dosage; D004331:Drosophila melanogaster / Q000502:physiology*; D006458:Hemolymph / Q000502:physiology; D018056:Hemorheology; D055620:Optical Phenomena; D012212:Rheology / Q000379:methods; D041623:Tomography, Optical Coherence / Q000379:methods*
D003287:Contrast Media