[Sequence and promoter efficacy analysis of avian leukosis virus subgroup J strains of different pathotypes].
To characterize the long terminal repeat (LTR) of the ALV-J strain which can induce hemangioma, fragments of provirus LTR of the three different ALV-J strains SCAU-HN06, NX0101 and JS-nt were amplified with a pair of specific primers, then cloned and subjected to sequence analysis. In comparison with the prototype ALV-J strains HPRS-103 and ADOL-7501, the LTRs of domestic strains (SCAU-HN06, NX0101, JS-nt and SD07lk1) had an 88.0%-97.2% nucleotide sequence identity; the U5 and R regions in the LTR had a high nucleotide similarity, while the U3 region in the LTR showed significant variance. The LTR fragments from the different ALV-J strains were inserted into the upstream of bacterial CAT gene of the plasmid pCAT-Basic, respectively. The resultant recombinant plasmids were transfected into DF-1 cells. The transfected cells were harvested 48 h post-transfection, and cell lysates were prepared for CAT expression detection. The CAT assay was performed using CAT-ELISA. The results showed that the promoter activity of the LTRSCAU-HNO6 was a little higher than those of LTRJS-nt and LTRNX0101, but there was no significant difference in the promoter activity among the compared LTRs.
College of Veterinary Medicine, South China Agricultural University, Key Laboratory of Animal Disease Control and Prevention, Ministry of Agriculture, Guang zhou 510642, China. zhanghenan66@yahoo.com.cn
He-nan
HN
Zhang
Yan
Y
Qi
Wei-wei
WW
Shi
Yi-yu
YY
Liang
Hong-bo
HB
Liu
Wei-sheng
WS
Cao
Ming
M
Liao
D000818:Animals; D001354:Avian Leukosis Virus / Q000145:classification / Q000235:genetics*; D001483:Base Sequence; D002645:Chickens; D008969:Molecular Sequence Data; D010802:Phylogeny; D011401:Promoter Regions, Genetic / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid