Expressions of protein oxidation markers, dityrosine and advanced oxidation protein products in Cisplatin-induced nephrotoxicity in rats.
The purpose of this study was to evaluate whether dityrosine and advanced oxidation protein products (AOPP) reflect the severity of cisplatin-induced nephrotoxicity. Immunoexpression of dityrosine in kidneys and plasma AOPP concentration were examined up to day 4 post-cisplatin injection in rats. Cisplatin injection induced tubular injury on days 2-4 after injection and increased serum creatinine and BUN on days 3 and 4. On days 2-4, dityrosine was immunostained in the cytoplasm of damaged tubular cells, and their immunostaining intensity increased time-dependently. Plasma AOPP levels were significantly increased on days 3 and 4. These results suggest that expressions of dityrosine and AOPP were associated with the severity of renal injury and may be useful markers for the development of cisplatin-induced nephrotoxicity.
United Graduate School of Veterinary Science, Yamaguchi University, Yoshida.
Yumi
Y
Kimoto
Akihiko
A
Sugiyama
Masaaki
M
Nishinohara
Atsushi
A
Asano
Aino
A
Masuda
Tairin
T
Ochi
Takashi
T
Takeuchi
D000818:Animals; D000970:Antineoplastic Agents / Q000633:toxicity*; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D002945:Cisplatin / Q000633:toxicity*; D005786:Gene Expression Regulation / Q000502:physiology*; D007674:Kidney Diseases / Q000139:chemically induced* / Q000378:metabolism; D010084:Oxidation-Reduction; D051381:Rats; D013997:Time Factors; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000097:blood / Q000378:metabolism
D000970:Antineoplastic Agents; D015415:Biomarkers; D014443:Tyrosine; C007543:dityrosine; D002945:Cisplatin