Combined effect of paste containing casein phosphopeptide-amorphous calcium phosphate and fluoride on enamel lesions: an in vitro pH-cycling study.
OBJECTIVE
The purpose of this study was to show through enamel remineralization that a combination of casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) and fluoride is better than fluoride alone when the processing time for remineralization is short.


METHODS
The bovine enamel slabs (n=28) were subjected to pH cycling for 4 days. Paste containing CPP-ACP and/or fluoride was applied for 30 minutes daily. The sections were observed using a polarizing microscope and microradiographs.


RESULTS
In the groups treated solely with sodium fluoride (NaF) solution, tissue loss on the enamel surface was observed. On the other hand, in the groups that had been treated with a mixture of the NaF solution and CPP-ACP, the enamel surface was maintained.


CONCLUSIONS
These results show that casein phosphopeptide-amorphous calcium phosphate-containing paste has the ability to maintain the enamel surface; the combined use of CPP-ACP paste and fluoride enhances this ability, thereby reducing demineralization.
Department of Pediatric Dentistry, School of Life Dentistry at Tokyo, Nippon Dental University, Tokyo, Japan. k-ogata@tky.ndu.ac.jp
Kiyokazu
K
Ogata
Sachie
S
Warita
Kisaki
K
Shimazu
Tomomi
T
Kawakami
Kyoko
K
Aoyagi
Hiroyuki
H
Karibe
D000818:Animals; D002327:Cariostatic Agents / Q000627:therapeutic use*; D002364:Caseins / Q000627:therapeutic use*; D002417:Cattle; D003743:Dental Enamel; D004338:Drug Combinations; D008852:Microradiography; D008859:Microscopy, Polarization; D012969:Sodium Fluoride / Q000627:therapeutic use*; D017001:Tooth Demineralization / Q000628:therapy*; D014089:Tooth Remineralization / Q000379:methods*
D002327:Cariostatic Agents; D002364:Caseins; D004338:Drug Combinations; C447032:casein phosphopeptide-amorphous calcium phosphate nanocomplex; D012969:Sodium Fluoride