Different mechanisms between copper and iron in catecholamines-mediated oxidative DNA damage and disruption of gene expression in vitro.
Catechols produce reactive oxygen species (ROS) and induce oxidative DNA damage through reduction-oxidation reactions with metals such as copper. Here, we examined oxidative DNA damage by neurotransmitter catecholamines in the presence of copper or iron and evaluated the effects of this damage on gene expression in vitro. Dopamine induced strand breaks and base oxidation in calf thymus DNA in the presence of Cu(II) or Fe(III)-NTA (nitrilotriacetic acid). The extent of this damage was greater for Cu(II) than for Fe(III)-NTA. For the DNA damage induced by dopamine, the responsible reactive species were hydrogen peroxide and Cu(I) for Cu(II) and hydroxyl radicals and Fe(II) for Fe(III)-NTA. Cu(II) induced DNA conformational changes, but Fe(III)-NTA did not in the presence of dopamine. These differences indicate different modes of action between Cu and Fe-NTA with regard to the induction of DNA damage. Expression of the lacZ gene coded on plasmid DNA was inhibited depending on the extent of the oxidative damage and strand breaks. Endogenous catecholamines (dopamine, adrenaline, and noradrenaline) were more potent than catechols (no aminoalkyl side chains) or 3,4-dihydroxybenzylamine (aminomethyl side chain). These results suggest that the metal-mediated DNA damage induced by dopamine disrupts gene expression, and leukoaminochromes (further oxidation products of O-quinones having aminoethyl side chain) are involved in the DNA damage. These findings indicate a possibility that metal (especially iron and copper)-mediated oxidation of catecholamines plays an important role in the pathogenesis of neurodegenerative disorders including Parkinson's disease.
Faculty of Pharmacy, Meijo University, 150 Yagotoyama, Tempaku-ku, Nagoya 468-8503, Japan.
Yoshihiko
Y
Nishino
Motozumi
M
Ando
Rena
R
Makino
Koji
K
Ueda
Yoshinori
Y
Okamoto
Nakao
N
Kojima
D000818:Animals; D002395:Catecholamines / Q000633:toxicity*; D002396:Catechols / Q000633:toxicity; D002417:Cattle; D003300:Copper / Q000633:toxicity*; D004247:DNA / Q000378:metabolism; D004249:DNA Damage / Q000235:genetics*; D005290:Ferric Compounds / Q000633:toxicity*; D015870:Gene Expression / Q000187:drug effects*; D066298:In Vitro Techniques; D007763:Lac Operon / Q000187:drug effects*; D009571:Nitrilotriacetic Acid / Q000031:analogs & derivatives* / Q000633:toxicity; D010084:Oxidation-Reduction / Q000187:drug effects*; D017382:Reactive Oxygen Species / Q000378:metabolism
D002395:Catecholamines; D002396:Catechols; D005290:Ferric Compounds; D017382:Reactive Oxygen Species; D003300:Copper; D004247:DNA; C427203:calf thymus DNA; D009571:Nitrilotriacetic Acid; C020326:ferric nitrilotriacetate