Fully integrated lab-on-a-disc for simultaneous analysis of biochemistry and immunoassay from whole blood.
We report a fully integrated device that can perform both multiple biochemical analysis and sandwich type immunoassay simultaneously on a disc. The whole blood is applied directly to the disposable "lab-on-a-disc" containing different kinds of freeze-dried reagents for the blood chemistry analysis as well as reagents required for the immunoassay. The concentrations of different kinds of analytes are reported within 22 min by simply inserting a disc to a portable device. Using the innovative laser irradiated ferrowax microvalves together with the centrifugal microfluidics, the total process of plasma separation, metering, mixing, incubation, washing, and detection is fully automated. The analyzer is equipped with an optical detection module to measure absorbances at 10 different wavelengths to accommodate the various kinds of reaction protocols. Compared to the conventional blood analysis done in clinical laboratories, it is advantageous for point-of-care applications because it requires a smaller amount of blood (350 μL vs. 3 mL), takes less time (22 min vs. several days), does not require specially trained operators or expensive instruments to run biochemical analysis and immunoassay separately.
Samsung Electronics Co. Ltd., 416, Maetan-3Dong, Yeongtong-Gu, Suwon-City, Gyeonggi-Do 443-746, Korea.
Beom Seok
BS
Lee
Yang Ui
YU
Lee
Han-Sang
HS
Kim
Tae-Hyeong
TH
Kim
Jiwoon
J
Park
Jeong-Gun
JG
Lee
Jintae
J
Kim
Hanshin
H
Kim
Wee Gyo
WG
Lee
Yoon-Kyoung
YK
Cho
D001774:Blood Chemical Analysis / Q000191:economics / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D006801:Humans; D007118:Immunoassay / Q000191:economics / Q000295:instrumentation* / Q000379:methods; D046210:Microfluidic Analytical Techniques / Q000191:economics / Q000295:instrumentation* / Q000379:methods