A high-throughput approach for studying virus replication in yeast.
Viruses are intracellular pathogens that are dependent on viral and host factors for multiplication. Model hosts, such as yeast, can be very valuable in identifying host factors involved in viral replication. Yeast is also useful for studies on functional interactions of host factors with viral proteins and/or virus nucleic acids. The advantages of using yeast include the availability of a single gene-deletion library and the essential gene library (yTHC); the controllable small- or large-scale expression of viral proteins and nucleic acids; and the rapid growth of yeast strains. Procedures that facilitate high-throughput analysis of host factors and plant and animal RNA virus replication in yeast, with a plant virus (tombusvirus; TBSV) and an animal virus (nodavirus; FHV) as examples, are described.
University of Kentucky, Lexington, Kentucky, USA.
Judit
J
Pogany
Tadas
T
Panavas
Elena
E
Serviene
Muhammad Shah
MS
Nawaz-ul-Rehman
Peter
P
D Nagy
D030381:Nodaviridae / Q000254:growth & development / Q000502:physiology*; D012441:Saccharomyces cerevisiae / Q000821:virology*; D017864:Tombusvirus / Q000254:growth & development / Q000502:physiology*; D014773:Virology / Q000379:methods*; D014779:Virus Replication