title: Influence of AIN-93 diet on mortality and cardiac remodeling after myocardial infarction in rats.
abstract: BACKGROUND
  The AIN-93 diet was proposed by the American Institute of Nutrition with the objective of standardising studies in experimental nutrition. Our objective was to analyze the effects of AIN-93 diet after myocardial infarction in rats.
  
  
  METHODS
  Post weaning, the animals were divided into two groups: control (C, n=62), fed the standard diet of our laboratory (Labina); AIN-93 Group (n=70), fed the AIN-93 diet. Achieving 250 g, the animals were subjected to myocardial infarction.
  
  
  RESULTS
  Early mortality was increased in AIN-93 animals, associated with lower serum levels of calcium, magnesium, potassium, sodium, and phosphorus. On the other hand, after 90 days, AIN-93 showed smaller normalized left ventricular dimensions. The caloric and carbohydrate intake was smaller, but the fat intake was higher in AIN-93 rats. AIN-93 group also showed increased levels of β-hydroxyacylcoenzyme A dehydrogenase and citrate synthase. In addition, serum levels of insulin and cardiac levels of malondialdehyde, metalloproteinases-2 and -9, and TNF-α and IFN-γ were decreased in the AIN-93 group.
  
  
  CONCLUSIONS
  AIN-93 diet increased early mortality, while attenuated the chronic remodeling process after experimental coronary occlusion. Therefore, this diet has biological effects and should be use with attention in this model.
authors.affiliation: Internal Medicine Department, Botucatu Medical School, UNESP-São Paulo State University, Botucatu, Brazil.
authors.forename: Lidiane P
authors.initials: LP
authors.lastname: Ardisson
authors.forename: Marcos F
authors.initials: MF
authors.lastname: Minicucci
authors.forename: Paula S
authors.initials: PS
authors.lastname: Azevedo
authors.forename: Fernanda
authors.initials: F
authors.lastname: Chiuso-Minicucci
authors.forename: Beatriz B
authors.initials: BB
authors.lastname: Matsubara
authors.forename: Luiz S
authors.initials: LS
authors.lastname: Matsubara
authors.forename: Priscila P
authors.initials: PP
authors.lastname: Santos
authors.forename: Heloisa B
authors.initials: HB
authors.lastname: Assalin
authors.forename: Rosangela
authors.initials: R
authors.lastname: Novo
authors.forename: Ethel L
authors.initials: EL
authors.lastname: Novelli
authors.forename: Léa Silvia
authors.initials: LS
authors.lastname: Sant'ana
authors.forename: Sergio A R
authors.initials: SA
authors.lastname: Paiva
authors.forename: Leonardo A M
authors.initials: LA
authors.lastname: Zornoff
mesh_terms: D000818:Animals; D004032:Diet / Q000009:adverse effects*; D008297:Male; D008903:Minerals / Q000008:administration & dosage / Q000097:blood*; D009203:Myocardial Infarction / Q000097:blood* / Q000401:mortality*; D009751:Nutritional Requirements; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D020257:Ventricular Remodeling / Q000502:physiology*
chemical_list: D008903:Minerals