title: Isolation of oligotrophic denitrifiers carrying previously uncharacterized functional gene sequences.
abstract: Oligotrophic denitrifying bacteria, including those belonging to the genera Herbaspirillum, Azospirillum, and Bradyrhizobium, were obtained using a single-cell isolation technique. The taxonomic composition of the denitrifier population was similar to those assessed by previous culture-independent studies. The sequencing of nitrite reductase and N(2)O reductase genes of these strains revealed previously unknown links between 16S rRNA and the denitrification-functional gene phylogenies. In particular, we identified Bradyrhizobium strains that harbor nirS sequences previously detected only in culture-independent studies.
authors.affiliation: Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan. anaerobe@mail.ecc.u-tokyo.ac.jp
authors.forename: Satoshi
authors.initials: S
authors.lastname: Ishii
authors.forename: Naoaki
authors.initials: N
authors.lastname: Ashida
authors.forename: Shigeto
authors.initials: S
authors.lastname: Otsuka
authors.forename: Keishi
authors.initials: K
authors.lastname: Senoo
mesh_terms: D016949:Azospirillum / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D020369:Bradyrhizobium / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D058440:Denitrification; D005798:Genes, Bacterial; D042803:Herbaspirillum / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D053858:Metabolic Networks and Pathways / Q000235:genetics*; D008969:Molecular Sequence Data; D009572:Nitrite Reductases / Q000235:genetics; D010088:Oxidoreductases / Q000235:genetics; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D010088:Oxidoreductases; D009572:Nitrite Reductases; C034747:nitric-oxide reductase