title: Differences in islet-enriched miRNAs in healthy and glucose intolerant human subjects.
abstract: Many microRNAs (miRNAs) are known to be cell-type specific and are implicated in development of diseases. We investigated the global expression pattern of miRNAs in human pancreatic islets compared to liver and skeletal muscle, using bead-based technology and quantitative RT-PCR. In addition to the known islet-specific miR-375, we also found enrichment of miR-127-3p, miR-184, miR-195 and miR-493∗ in the pancreatic islets. The expression of miR-375, miR-127-3p, miR-184 and the liver-enriched miR-122 is positively correlated to insulin biosynthesis, while the expression of miR-127-3p and miR-184 is negatively correlated to glucose-stimulated insulin secretion (GSIS). These correlations were absent in islets of glucose intolerant donors (HbA1c ≥ 6.1). We suggest that the presence of an islet-specific miRNA network, which consists of at least miR-375, miR-127-3p and miR-184, potentially involved in insulin secretion. Our results provide new insight into miRNA-mediated regulation of insulin secretion in healthy and glucose intolerant subjects.
authors.affiliation: Cellular Autoimmunity Unit, Department of Clinical Sciences in Malmö, Lund University, Malmö University Hospital, Malmö, Sweden.
authors.forename: Caroline
authors.initials: C
authors.lastname: Bolmeson
authors.forename: Jonathan L S
authors.initials: JL
authors.lastname: Esguerra
authors.forename: Albert
authors.initials: A
authors.lastname: Salehi
authors.forename: Dina
authors.initials: D
authors.lastname: Speidel
authors.forename: Lena
authors.initials: L
authors.lastname: Eliasson
authors.forename: Corrado M
authors.initials: CM
authors.lastname: Cilio
mesh_terms: D018149:Glucose Intolerance / Q000378:metabolism*; D006801:Humans; D007328:Insulin / Q000096:biosynthesis / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D035683:MicroRNAs / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000378:metabolism
chemical_list: D007328:Insulin; D035683:MicroRNAs