title: Preliminary investigations on laminin coatings for flexible polyimide/platinum thin films for PNS applications.
abstract: The aim of this work was to investigate the possibility to obtain stable bioactive coatings for polyimide/platinum neural interfaces based on thin film technology for applications into the peripheral nervous system (PNS). Laminin (LI), a glycoprotein of the extracellular matrix, which guides and promotes differentiation and growth of neurons, was selected to deposit bioactive coatings. Dip-coating was performed on dummy structures at different LI concentrations. Indirect methods allowed to identify and characterize laminin on coated samples. Mechanical stability was also confirmed by indirect evaluations. Pilot experiments with differentiated PC12 cells, by the addition of nerve growth factor (NGF), showed improved neurite outgrowth on the coated probes compared to bare polyimide samples.
authors.affiliation: Advanced Robotics Technology and Systems Laboratory, Scuola Superiore Sant'Anna, Pontedera -56025, Italy. s.bossi@sssup.it
authors.forename: S
authors.initials: S
authors.lastname: Bossi
authors.forename: A
authors.initials: A
authors.lastname: Benvenuto
authors.forename: P
authors.initials: P
authors.lastname: Wieringa
authors.forename: G
authors.initials: G
authors.lastname: Di Pino
authors.forename: E
authors.initials: E
authors.lastname: Guglielmelli
authors.forename: T
authors.initials: T
authors.lastname: Boretius
authors.forename: T
authors.initials: T
authors.lastname: Stieglitz
authors.forename: X
authors.initials: X
authors.lastname: Navarro
authors.forename: S
authors.initials: S
authors.lastname: Micera
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis* / Q000494:pharmacology; D055119:Elastic Modulus; D004567:Electrodes, Implanted; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D007797:Laminin / Q000737:chemistry* / Q000494:pharmacology; D008422:Materials Testing; D008567:Membranes, Artificial; D016716:PC12 Cells / Q000187:drug effects; D010363:Pattern Recognition, Automated / Q000379:methods*; D010525:Peripheral Nerves / Q000502:physiology; D010984:Platinum / Q000737:chemistry*; D051381:Rats; D012117:Resins, Synthetic / Q000737:chemistry*
chemical_list: D020099:Coated Materials, Biocompatible; D007797:Laminin; D008567:Membranes, Artificial; D012117:Resins, Synthetic; C029945:polyimide resin; D010984:Platinum