title: An optimized RGD-decorated micellar drug delivery system for albendazole for the treatment of ovarian cancer: from RAFT polymer synthesis to cellular uptake.
abstract: Block copolymers were prepared via RAFT polymerization with P(PEGMEMA) as the hydrophilic block to form micelles for the controlled delivery of ABZ. The group contribution method was used to estimate the partial solubility parameters for ABZ and various polymers as potential core-forming block to achieve optimum compatibility. Different ratios between MMA and LMA, a non-compatible monomer, were prepared. Cytotoxicity tests revealed a high toxicity of the ABZ-loaded micelle resulting in 80% cell deaths at a micelle concentration of 10 µg · mL(-1) . Cellular uptake of micelles has been studied using fluorescently labeled micelles, showing that a large fraction of micelles is readily taken up by OVCAR-3 cells. RGD-conjugated micelles were prepared and showed an increased cellular uptake.
authors.affiliation: Centre for Advanced Macromolecular Design (CAMD), University of New South Wales, Sydney, NSW 2052, Australia.
authors.forename: Yoseop
authors.initials: Y
authors.lastname: Kim
authors.forename: Mohammad H
authors.initials: MH
authors.lastname: Pourgholami
authors.forename: David L
authors.initials: DL
authors.lastname: Morris
authors.forename: Martina H
authors.initials: MH
authors.lastname: Stenzel
mesh_terms: D015766:Albendazole / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology / Q000627:therapeutic use*; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002850:Chromatography, Gel; D016503:Drug Delivery Systems / Q000379:methods*; D005260:Female; D006801:Humans; D057446:Hydrodynamics; D008823:Micelles; D008856:Microscopy, Fluorescence; D008970:Molecular Weight; D009842:Oligopeptides / Q000737:chemistry*; D010051:Ovarian Neoplasms / Q000188:drug therapy* / Q000473:pathology; D058105:Polymerization / Q000187:drug effects; D011108:Polymers / Q000138:chemical synthesis* / Q000737:chemistry; D011109:Polymethacrylic Acids / Q000138:chemical synthesis / Q000737:chemistry; D012995:Solubility / Q000187:drug effects; D013500:Surface Tension / Q000187:drug effects
chemical_list: D008823:Micelles; D009842:Oligopeptides; D011108:Polymers; D011109:Polymethacrylic Acids; C047981:arginyl-glycyl-aspartic acid; D015766:Albendazole