title: Functional assignments for the carboxyl-terminal domains of the ferrochelatase from Synechocystis PCC 6803: the CAB domain plays a regulatory role, and region II is essential for catalysis.
abstract: Ferrochelatase (FeCH) catalyzes the insertion of Fe(2+) into protoporphyrin, forming protoheme. In photosynthetic organisms, FeCH and magnesium chelatase lie at a biosynthetic branch point where partitioning down the heme and chlorophyll (Chl) pathways occurs. Unlike their mammalian, yeast, and other bacterial counterparts, cyanobacterial and algal FeCHs as well as FeCH2 isoform from plants possess a carboxyl-terminal Chl a/b-binding (CAB) domain with a conserved Chl-binding motif. The CAB domain is connected to the FeCH catalytic core by a proline-rich linker sequence (region II). In order to dissect the regulatory, catalytic, and structural roles of the region II and CAB domains, we analyzed a FeCH ΔH347 mutant that retains region II but lacks the CAB domain and compared it with the ΔH324-FeCH mutant that lacks both these domains. We found that the CAB domain is not required for catalytic activity but is essential for dimerization of FeCH; its absence causes aberrant accumulation of Chl-protein complexes under high light accompanied by high levels of the Chl precursor chlorophyllide. Thus, the CAB domain appears to serve mainly a regulatory function, possibly in balancing Chl biosynthesis with the synthesis of cognate apoproteins. Region II is essential for the catalytic function of the plastid-type FeCH enzyme, although the low residual activity of the ΔH324-FeCH is more than sufficient to furnish the cellular demand for heme. We propose that the apparent surplus of FeCH activity in the wild type is critical for cell viability under high light due to a regulatory role of FeCH in the distribution of Chl into apoproteins.
authors.affiliation: Institute of Microbiology, Department of Autotrophic Microorganisms, 379 81 Trebon, Czech Republic. sobotka@alga.cz
authors.forename: Roman
authors.initials: R
authors.lastname: Sobotka
authors.forename: Martin
authors.initials: M
authors.lastname: Tichy
authors.forename: Annegret
authors.initials: A
authors.lastname: Wilde
authors.forename: C Neil
authors.initials: CN
authors.lastname: Hunter
mesh_terms: D000064:Acclimatization; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002734:Chlorophyll / Q000096:biosynthesis; D005294:Ferrochelatase / Q000235:genetics / Q000378:metabolism*; D008027:Light; D009154:Mutation; D054730:Protein Interaction Domains and Motifs; D055503:Protein Multimerization; D046939:Synechocystis / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D045725:Tetrapyrroles / Q000096:biosynthesis
chemical_list: D001426:Bacterial Proteins; D045725:Tetrapyrroles; D002734:Chlorophyll; D005294:Ferrochelatase