title: Differential liver proteome mapping of control and cadmium-fed rats.
abstract: A comparative study of proteome maps from control and Cd-exposed rat liver was performed using a new technology of two-dimensional liquid chromatography separation method (PF-2D system, Beckman Coulter). Rats were fed for one month 0 or 100 μg Cd g(-1). The between-replicate and between-sample variations showed good repeatability and suitable reproducibility for the two dimensions of separation of proteins. In this complex mixture, PF-2D led to the separation of two major peaks which differed between control and Cd-exposed rat livers, one being identified by mass spectrometry as Cu/Zn superoxide dismutase (SOD), a well-known biomarker of Cd exposure, the other as phosphatidylethanolamine binding protein (PEBP). SOD content was decreased in Cd-exposed rat liver, compared to the control group which was corroborated by a significant decrease of SOD activity. PEBP content also tended to be decreased after Cd exposure. Present results demonstrate interest but also limitations of proteomic approach using PF-2D system to analyze effects of chemicals on organisms.
authors.affiliation: Université de Franche-Comté, Laboratoire de Chrono-Environnement, UMR UFC/CNRS 6249 USC INRA, Place Leclerc, 25030 Besançon cedex, France.
authors.forename: F
authors.initials: F
authors.lastname: Hispard
authors.forename: A
authors.initials: A
authors.lastname: de Vaufleury
authors.forename: C
authors.initials: C
authors.lastname: Schaeffer
authors.forename: R
authors.initials: R
authors.lastname: Scheifler
authors.forename: P-M
authors.initials: PM
authors.lastname: Badot
authors.forename: L
authors.initials: L
authors.lastname: Richert
authors.forename: H
authors.initials: H
authors.lastname: Martin
mesh_terms: D000818:Animals; D002104:Cadmium / Q000378:metabolism / Q000633:toxicity*; D015180:Electrophoresis, Gel, Two-Dimensional; D004785:Environmental Pollutants / Q000378:metabolism / Q000633:toxicity*; D005260:Female; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D049488:Phosphatidylethanolamine Binding Protein / Q000378:metabolism; D025941:Protein Interaction Mapping; D020543:Proteome / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D004785:Environmental Pollutants; D049488:Phosphatidylethanolamine Binding Protein; D020543:Proteome; D017392:Thiobarbituric Acid Reactive Substances; D002104:Cadmium; D013482:Superoxide Dismutase