title: Analytical determination and pharmacokinetics of major metabolites of carbasalate calcium in broilers following oral administration.
abstract: As a newer anti-inflammatory agent, carbasalate calcium is used in various animal species. In this study, the pharmacokinetics of carbasalate calcium was investigated in broilers. Broilers, with body weight of 2.0 ± 0.3 kg, were administrated carbasalate calcium soluble powder at a single dose of 40 mg/kg body weight orally. The plasma concentrations of its metabolites, aspirin (ASA), salicylic acid (SA) and gentisic acid (GA) were determined by LC-MS/MS method and the pharmacokinetic parameters were calculated by noncompartmental analysis. After oral administration of carbasalate calcium, the plasma drug concentration for ASA, SA and GA reached a peak (C(max) ) of 8.88 ± 1.31, 42.6 ± 4.62 and 10.1 ± 2.16 μg/mL at 0.170, 2.00 and 2.00 h, respectively. The terminal half-life (t(1/2λz) ) of ASA, SA and GA was 11.2 ± 8.04, 23.7 ± 17.1 and 28.6 ± 4.90 h, respectively. In conclusion, analytical method for the quantification of ASA, SA and GA in plasma in the broilers was developed and validated. In broilers, carbasalate calcium is quickly metabolized in ASA and ASA is rapidly converted to SA and one of the metabolites of SA is GA.
authors.affiliation: National Reference Laboratory of Veterinary Drug Residues and MOA Key Laboratory of Food Safety Evaluation, Huazhong Agricultural University, Wuhan, China.
authors.forename: X
authors.initials: X
authors.lastname: Wang
authors.forename: L-L
authors.initials: LL
authors.lastname: Huang
authors.forename: D-M
authors.initials: DM
authors.lastname: Chen
authors.forename: A
authors.initials: A
authors.lastname: Ihsan
authors.forename: Z-H
authors.initials: ZH
authors.lastname: Yuan
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage / Q000378:metabolism / Q000493:pharmacokinetics*; D001241:Aspirin / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000097:blood / Q000378:metabolism / Q000493:pharmacokinetics; D002645:Chickens / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000662:veterinary; D002853:Chromatography, Liquid / Q000662:veterinary; D004334:Drug Administration Schedule / Q000662:veterinary; D005260:Female; D005841:Gentisates / Q000097:blood; D008297:Male; D020156:Salicylic Acid / Q000097:blood; D053719:Tandem Mass Spectrometry / Q000662:veterinary; D014508:Urea / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000378:metabolism / Q000493:pharmacokinetics
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D005841:Gentisates; D014508:Urea; C002942:carbaspirin calcium; D020156:Salicylic Acid; D001241:Aspirin; C010925:2,5-dihydroxybenzoic acid