title: The proline/arginine-rich domain is a major determinant of dynamin self-activation.
abstract: Dynamins induce membrane vesiculation during endocytosis and Golgi budding in a process that requires assembly-dependent GTPase activation. Brain-specific dynamin 1 has a weaker propensity to self-assemble and self-activate than ubiquitously expressed dynamin 2. Here we show that dynamin 3, which has important functions in neuronal synapses, shares the self-assembly and GTPase activation characteristics of dynamin 2. Analysis of dynamin hybrids and of dynamin 1-dynamin 2 and dynamin 1-dynamin 3 heteropolymers reveals that concentration-dependent GTPase activation is suppressed by the C-terminal proline/arginine-rich domain of dynamin 1. Dynamin proline/arginine-rich domains also mediate interactions with SH3 domain-containing proteins and thus regulate both self-association and heteroassociation of dynamins.
authors.affiliation: Department of Pharmacology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, Texas 75235-9041, United States.
authors.forename: Barbara
authors.initials: B
authors.lastname: Barylko
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Derk D
authors.initials: DD
authors.lastname: Binns
authors.forename: Justin A
authors.initials: JA
authors.lastname: Ross
authors.forename: Tara C
authors.initials: TC
authors.lastname: Tassin
authors.forename: Katie A
authors.initials: KA
authors.lastname: Collins
authors.forename: David M
authors.initials: DM
authors.lastname: Jameson
authors.forename: Joseph P
authors.initials: JP
authors.lastname: Albanesi
mesh_terms: D000818:Animals; D001120:Arginine / Q000737:chemistry*; D002460:Cell Line; D034284:Dynamin I / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D034285:Dynamin II / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D034286:Dynamin III / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D034281:Dynamins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D011392:Proline / Q000737:chemistry*; D055232:Proline-Rich Protein Domains / Q000235:genetics / Q000502:physiology; D017434:Protein Structure, Tertiary; D018411:Spodoptera
chemical_list: D001120:Arginine; D011392:Proline; D034284:Dynamin I; D034285:Dynamin II; D034286:Dynamin III; D034281:Dynamins