title: Segmental cholangitis impairs hepatic regeneration capacity after partial hepatectomy in rats.
abstract: BACKGROUND
  Patients with hilar cholangiocarcinoma or hepatolithiasis often develop segmental cholangitis (SC), but it is unclear whether hepatectomy for patients with SC can be performed safely.
  
  
  METHODS
  Rats were subjected to segmental bile duct ligation (SBDL) with LPS (SC group) or a saline (Sham group) infusion into the bile duct of the ligated lobes. The rats were sacrificed at 3, 24 and 48 h after the SBDL. For another experiment, the rats were subjected to partial hepatectomy (PHx) for the ligated lobes. Hepatic regeneration rates and the expression of regeneration-associated genes were evaluated.
  
  
  RESULTS
  In the SC group, severe parenchymal damage was observed in the acute phase (3 h). Altered gene expression in the liver in response to biliary infection occurred not only in the infected lobes but also in the non-infected lobes. In the rats of the SC group, both the hepatic regeneration rate and serum HGF levels were significantly lower than in the Sham group.
  
  
  CONCLUSIONS
  These results clearly demonstrate that SC impairs the regeneration capacity of the contralateral remnant liver. Therefore, hepatectomy should be avoided for patients with SC even if it occurs in the part of the liver to be resected.
authors.affiliation: Division of Surgical Oncology, Department of Surgery, Nagoya University Graduate School of Medicine, Nagoya, Japan.
authors.forename: Katsutaka
authors.initials: K
authors.lastname: Watanabe
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Yokoyama
authors.forename: Toshio
authors.initials: T
authors.lastname: Kokuryo
authors.forename: Kiyotaka
authors.initials: K
authors.lastname: Kawai
authors.forename: Tomomi
authors.initials: T
authors.lastname: Kitagawa
authors.forename: Takashi
authors.initials: T
authors.lastname: Seki
authors.forename: Akifumi
authors.initials: A
authors.lastname: Nakagawa
authors.forename: Masato
authors.initials: M
authors.lastname: Nagino
mesh_terms: D000199:Actins / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001652:Bile Ducts / Q000601:surgery; D015415:Biomarkers / Q000097:blood; D049109:Cell Proliferation; D002761:Cholangitis / Q000097:blood / Q000209:etiology / Q000503:physiopathology*; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D005786:Gene Expression Regulation; D006498:Hepatectomy; D007150:Immunohistochemistry; D018836:Inflammation Mediators / Q000378:metabolism; D008026:Ligation; D008070:Lipopolysaccharides; D008099:Liver / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology* / Q000601:surgery*; D008111:Liver Function Tests; D008115:Liver Regeneration / Q000235:genetics; D008297:Male; D008940:Mitotic Index; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors
chemical_list: D000199:Actins; D015415:Biomarkers; D018836:Inflammation Mediators; D008070:Lipopolysaccharides; D012333:RNA, Messenger; C440499:lipopolysaccharide, Escherichia coli O111 B4; C498862:Acta2 protein, rat