title: Attempt at cloning high-quality goldfish breed 'Ranchu' by fin-cultured cell nuclear transplantation.
abstract: The viability of ornamental fish culture relies on the maintenance of high-quality breeds. To improve the profitability of culture operations we attempted to produce cloned fish from the somatic nucleus of the high-quality Japanese goldfish (Carassius auratus auratus) breed 'Ranchu'. We transplanted the nucleus of a cultured fin-cell from an adult Ranchu into the non-enucleated egg of the original goldfish breed 'Wakin'. Of the 2323 eggs we treated, 802 underwent cleavage, 321 reached the blastula stage, and 51 reached the gastrula stage. Two of the gastrulas developed until the hatching stage. A considerable number of nuclear transplants retained only the donor nucleus. Some of these had only a 2n nucleus derived from the same donor fish. Our results provide insights into the process of somatic cell nuclear transplantation in teleosts, and the cloning of Ranchu.
authors.affiliation: Department of Fisheries Science, Kinki University, Nara, Nara 6318505, Japan. planarian@live.jp
authors.forename: Daisuke
authors.initials: D
authors.lastname: Tanaka
authors.forename: Akito
authors.initials: A
authors.lastname: Takahashi
authors.forename: Akinori
authors.initials: A
authors.lastname: Takai
authors.forename: Hiromi
authors.initials: H
authors.lastname: Ohta
authors.forename: Koichi
authors.initials: K
authors.lastname: Ueno
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism*; D019976:Cloning, Organism / Q000379:methods*; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D047108:Embryonic Development; D005260:Female; D018507:Gene Expression Regulation, Developmental; D005818:Genetic Engineering / Q000379:methods*; D006054:Goldfish / Q000196:embryology / Q000235:genetics* / Q000378:metabolism; D008297:Male; D053652:Nuclear Transfer Techniques; D010063:Ovum / Q000166:cytology / Q000378:metabolism; D011123:Polyploidy; D019105:Random Amplified Polymorphic DNA Technique