title: A novel serine alkaline protease from Bacillus altitudinis GVC11 and its application as a dehairing agent.
abstract: A serine alkaline protease from a newly isolated alkaliphilic Bacillus altitudinis GVC11 was purified and characterized. The enzyme was purified to homogeneity by acetone precipitation, DEAE-cellulose anion exchange chromatography with 7.03-fold increase in specific activity and 15.25% recovery. The molecular weight of alkaline protease was estimated to be 28 kDa by SDS PAGE and activity was further assessed by zymogram analysis. The enzyme was highly active over a wide range of pH 8.5 to 12.5 with an optimum pH of 9.5. The optimum temperature of purified enzyme was 45 °C and Ca(2+) further increased the thermal stability of the enzyme. The enzyme activity was enhanced by Ca(2+) and Mg(2+) and inhibited by Hg(2+). The present study is the first report to examine and describe production of highly alkaline protease from Bacillus altitudinis and also its remarkable dehairing ability of goat hide in 18 h without disturbing the collagen and hair integrity.
authors.affiliation: Department of Microbiology, Osmania University, Hyderabad, India. evrbio81@gmail.com
authors.forename: E
authors.initials: E
authors.lastname: Vijay Kumar
authors.forename: M
authors.initials: M
authors.lastname: Srijana
authors.forename: K
authors.initials: K
authors.lastname: Kiran Kumar
authors.forename: N
authors.initials: N
authors.lastname: Harikrishna
authors.forename: Gopal
authors.initials: G
authors.lastname: Reddy
mesh_terms: D000818:Animals; D001407:Bacillus / Q000201:enzymology*; D001426:Bacterial Proteins / Q000737:chemistry*; D001709:Biotechnology / Q000379:methods; D002118:Calcium / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases / Q000737:chemistry*; D005285:Fermentation; D006041:Goats; D006197:Hair; D006863:Hydrogen-Ion Concentration; D007477:Ions; D008274:Magnesium / Q000737:chemistry; D008628:Mercury / Q000737:chemistry; D013633:Tanning / Q000379:methods*; D013696:Temperature
chemical_list: D001426:Bacterial Proteins; D007477:Ions; D010450:Endopeptidases; C087822:alkaline protease; D008628:Mercury; D008274:Magnesium; D002118:Calcium