title: Pore-forming activity of BAD is regulated by specific phosphorylation and structural transitions of the C-terminal part.
abstract: BACKGROUND
  BAD protein (Bcl-2 antagonist of cell death) belongs to the BH3-only subfamily of proapoptotic proteins and is proposed to function as the sentinel of the cellular health status. Physiological activity of BAD is regulated by phosphorylation, association with 14-3-3 proteins, binding to membrane lipids and pore formation. Since the functional role of the BAD C-terminal part has not been considered so far, we have investigated here the interplay of the structure and function of this region.
  
  
  METHODS
  The structure of the regulatory C-terminal part of human BAD was analyzed by CD spectroscopy. The channel-forming activity of full-length BAD and BAD peptides was carried out by lipid bilayer measurements. Interactions between proteins and peptides were monitored by the surface plasmon resonance technique. In aqueous solution, C-terminal part of BAD exhibits a well-ordered structure and stable conformation. In a lipid environment, the helical propensity considerably increases. The interaction of the C-terminal segment of BAD with the isolated BH3 domain results in the formation of permanently open pores whereby the phosphorylation of serine 118 within the BH3 domain is necessary for effective pore formation. In contrast, phosphorylation of serine 99 in combination with 14-3-3 association suppresses formation of channels. C-terminal part of BAD controls BAD function by structural transitions, lipid binding and phosphorylation. Conformational changes of this region upon membrane interaction in conjunction with phosphorylation of the BH3 domain suggest a novel mechanism for regulation of BAD.
  
  
  CONCLUSIONS
  Multiple signaling pathways mediate inhibition and activation of cell death via BAD.
authors.affiliation: Theodor Boveri Institute, Department of Microbiology, University of Wuerzburg, 97074 Wuerzburg, Germany. lisa.polzien@uni-wuerzburg.de
authors.forename: Lisa
authors.initials: L
authors.lastname: Polzien
authors.forename: Angela
authors.initials: A
authors.lastname: Baljuls
authors.forename: Heide-Marie
authors.initials: HM
authors.lastname: Roth
authors.forename: Jochen
authors.initials: J
authors.lastname: Kuper
authors.forename: Roland
authors.initials: R
authors.lastname: Benz
authors.forename: Kristian
authors.initials: K
authors.lastname: Schweimer
authors.forename: Mirko
authors.initials: M
authors.lastname: Hekman
authors.forename: Ulf R
authors.initials: UR
authors.lastname: Rapp
mesh_terms: D048948:14-3-3 Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D000595:Amino Acid Sequence; D002942:Circular Dichroism; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008051:Lipid Bilayers / Q000737:chemistry* / Q000378:metabolism; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D010766:Phosphorylation; D011485:Protein Binding; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D020349:Surface Plasmon Resonance; D014867:Water / Q000737:chemistry; D051025:bcl-Associated Death Protein / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D048948:14-3-3 Proteins; D008051:Lipid Bilayers; D010455:Peptides; D011993:Recombinant Fusion Proteins; D051025:bcl-Associated Death Protein; D014867:Water; D005982:Glutathione Transferase