title: Baculovirus vector-mediated transfer of NIS gene into colon tumor cells for radionuclide therapy.
abstract: OBJECTIVE
  To investigate the feasibility of radionuclide therapy of colon tumor cells by baculovirus vector-mediated transfer of the sodium/iodide symporter (NIS) gene.
  
  
  METHODS
  A recombinant baculovirus plasmid carrying the NIS gene was constructed, and the viruses (Bac-NIS) were prepared using the Bac-to-Bac system. The infection efficiency in the colon cancer cell line SW1116 of a green fluorescent protein (GFP) expressing baculovirus (Bac-GFP) at different multiplicities of infection (MOI) with various concentrations of sodium butyrate was determined by flow cytometry. An in vitro cytotoxicity assay was also conducted after infection of SW1116 cells with Bac-NIS. Iodine uptake of Bac-NIS infected SW1116 cells and inhibition of this uptake by sodium perchlorate was examined, and the effect of Bac-NIS-mediated (131)I in killing tumor cells was evaluated by cell colony formation tests.
  
  
  RESULTS
  Infection and transgene expression in SW1116 with Bac-GFP were significantly enhanced by sodium butyrate, as up to 72% of SW1116 cells were infected with the virus at MOI of 400 and sodium butyrate at 0.5 mmol/L. No obvious cytotoxicity was observed under these conditions. Infection of SW1116 with Bac-NIS allowed uptake of (131)I in these tumor cells, which could be inhibited by sodium perchlorate. The viability of SW1116 cells infected with Bac-NIS was significantly lower than with Bac-GFP, suggesting that NIS gene-mediated (131)I uptake could specifically kill tumor cells.
  
  
  CONCLUSIONS
  Baculovirus vector-mediated NIS gene therapy is a potential approach for treatment of colon cancer.
authors.affiliation: Department of Nuclear Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, No. 197, Rui Jin 2nd Road, Shanghai 200025, China.
authors.forename: Hong-Yan
authors.initials: HY
authors.lastname: Yin
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhou
authors.forename: Hai-Fei
authors.initials: HF
authors.lastname: Wu
authors.forename: Biao
authors.initials: B
authors.lastname: Li
authors.forename: Yi-Fan
authors.initials: YF
authors.lastname: Zhang
mesh_terms: D016367:Baculoviridae / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000000981:diagnostic imaging / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007457:Iodine Radioisotopes / Q000378:metabolism; D011877:Radionuclide Imaging; D011878:Radiotherapy / Q000379:methods*; D027981:Symporters / Q000235:genetics* / Q000378:metabolism; D014162:Transfection; D000099209:Sodium-Iodide Symporters
chemical_list: D049452:Green Fluorescent Proteins; D007457:Iodine Radioisotopes; D027981:Symporters; D000099209:Sodium-Iodide Symporters