title: Structural models of DYNLL1 with interacting partners: African swine fever virus protein p54 and postsynaptic scaffolding protein gephyrin.
abstract: DYNLL1, the smallest dynein light chain, interacts with different cargos facilitating their cellular transport. Usually the sequence recognized in the targets is homologous to the GIQVD or the KXTQT motifs with a glutamine that is important for binding. Here we add two new examples of DYNLL1 targets that can be classified into these two groups: ASFV p54 and gephyrin. Using NMR we demonstrate the direct interaction between DYNLL1 and two peptides derived from their interacting sequences. We model the structure of both complexes and show that the overall binding mode is preserved as in other complexes despite differences at the residue-specific interactions.
authors.affiliation: Departamento de Química Física Biológica, Instituto de Química Física Rocasolano, CSIC, Madrid, Spain. mgarcia@iqfr.csic.es
authors.forename: María Flor
authors.initials: MF
authors.lastname: García-Mayoral
authors.forename: I
authors.initials: I
authors.lastname: Rodríguez-Crespo
authors.forename: M
authors.initials: M
authors.lastname: Bruix
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites / Q000235:genetics; D002352:Carrier Proteins / Q000737:chemistry* / Q000378:metabolism; D056445:Cytoplasmic Dyneins / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008565:Membrane Proteins / Q000737:chemistry* / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D017386:Sequence Homology, Amino Acid; D013569:Synapses / Q000378:metabolism; D015678:Viral Structural Proteins / Q000737:chemistry* / Q000378:metabolism
chemical_list: D002352:Carrier Proteins; D008565:Membrane Proteins; D009842:Oligopeptides; D015678:Viral Structural Proteins; C073441:gephyrin; C093921:p54 protein, African swine fever virus; C427758:DYNLL1 protein, human; D056445:Cytoplasmic Dyneins