title: Development and validation of an HPLC-MS/MS method for the analysis of dexamethasone from pig synovial fluid using dried matrix spotting.
abstract: BACKGROUND
  Dried matrix spot techniques were employed to validate an HPLC-MS/MS assay for the determination of dexamethasone in clear Yorkshire pig synovial fluid using 15 µl of sample. We have adopted the term dried matrix spot to indicate that the techniques used for dried blood spots can be applied to nonblood matrices. The dried matrix spot method employs a color-indicating process developed at Alturas Analytics that enhances the ability to analyze transparent fluids spotted onto collection paper by allowing the analyst to visually verify the location of the dried sample spot.
  
  
  RESULTS
  The method was shown to be accurate (±4.3%) and precise (14.2% at the LLOQ and ≤10.0% at all other concentrations) across the dynamic range of the assay.
  
  
  CONCLUSIONS
  The method shows the potential application of dried matrix spot techniques for the analysis of transparent biological fluids.
authors.affiliation: Alturas Analytics, Inc, 1324 Alturas Drive, Moscow, ID 83843, USA. cchristianson@alturasanalytics.com
authors.forename: Chad D
authors.initials: CD
authors.lastname: Christianson
authors.forename: Derek F
authors.initials: DF
authors.lastname: Laine
authors.forename: Jennifer Sd
authors.initials: JS
authors.lastname: Zimmer
authors.forename: Casey Jl
authors.initials: CJ
authors.lastname: Johnson
authors.forename: Chrystal N
authors.initials: CN
authors.lastname: Sheaff
authors.forename: Anna
authors.initials: A
authors.lastname: Carpenter
authors.forename: Shane R
authors.initials: SR
authors.lastname: Needham
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003116:Color; D004396:Coloring Agents / Q000737:chemistry; D003907:Dexamethasone / Q000032:analysis* / Q000302:isolation & purification; D004355:Drug Stability; D012015:Reference Standards; D013048:Specimen Handling / Q000379:methods*; D013552:Swine; D013582:Synovial Fluid / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods* / Q000592:standards
chemical_list: D004396:Coloring Agents; D003907:Dexamethasone