title: Recognition of Hg(2+) using diametrically disubstituted cyclam unit.
abstract: A newly synthesized 1,4,8,11-tetraazacyclotetradecane derivative (L), functionalized with a diazo moiety as the reporter functionality, is found to bind specifically to Hg(2+) with an associated change in color that could be visually detected. With biologically benign β-CD, it forms an inclusion complex (L·2β-CD), which shows a much higher solubility in water, and this helps in developing a more intense color on binding to Hg(2+) in a CH(3)CN-HEPES buffer medium. The nontoxic nature of L was checked with the living cells of a Gram negative bacterium, Pseudomonas putida . Further, experiments revealed that these two reagents could be used as staining agents for the detection of Hg(2+) present in this microorganism.
authors.affiliation: Central Salt and Marine Chemical Research Institute, (CSIR), Bhavnagar -364002, Gujarat, India.
authors.forename: Prasenjit
authors.initials: P
authors.lastname: Mahato
authors.forename: Amrita
authors.initials: A
authors.lastname: Ghosh
authors.forename: Sukdeb
authors.initials: S
authors.lastname: Saha
authors.forename: Sandhya
authors.initials: S
authors.lastname: Mishra
authors.forename: Sanjiv K
authors.initials: SK
authors.lastname: Mishra
authors.forename: Amitava
authors.initials: A
authors.lastname: Das
mesh_terms: D001391:Azo Compounds / Q000737:chemistry; D006571:Heterocyclic Compounds / Q000737:chemistry* / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D008627:Mercuric Chloride / Q000032:analysis*; D016958:Pseudomonas putida / Q000187:drug effects; D021241:Spectrometry, Mass, Electrospray Ionization; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D047392:beta-Cyclodextrins / Q000737:chemistry
chemical_list: D001391:Azo Compounds; D006571:Heterocyclic Compounds; D047392:beta-Cyclodextrins; C024560:cyclam; D008627:Mercuric Chloride