title: Tyrosine phosphorylation enrichment and subsequent analysis by MALDI-TOF/TOF MS/MS and LC-ESI-IT-MS/MS.
abstract: This unit describes methods to detect, identify, and characterize tyrosine phosphorylation in proteins by mass spectrometry, including sample preparation methods, enrichment strategies using phosphotyrosine-specific antibodies, and chromatographic separation methods.
authors.affiliation: Adelaide Proteomics Centre, School of Molecular and Biomedical Science, University of Adelaide, Adelaide, Australia.
authors.forename: Mark R
authors.initials: MR
authors.lastname: Condina
authors.forename: Manuela
authors.initials: M
authors.lastname: Klingler-Hoffmann
authors.forename: Peter
authors.initials: P
authors.lastname: Hoffmann
mesh_terms: D045163:Antibodies, Phospho-Specific; D002623:Chemistry Techniques, Analytical; D002853:Chromatography, Liquid / Q000379:methods; D010449:Peptide Mapping / Q000379:methods; D010766:Phosphorylation; D019000:Phosphotyrosine / Q000032:analysis* / Q000737:chemistry; D011499:Protein Processing, Post-Translational; D011506:Proteins / Q000032:analysis / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D014443:Tyrosine / Q000737:chemistry
chemical_list: D045163:Antibodies, Phospho-Specific; D011506:Proteins; D019000:Phosphotyrosine; D014443:Tyrosine