title: Stimulation of osteoblastic differentiation and mineralization in MC3T3-E1 cells by yeast hydrolysate.
abstract: In a previous study, it was reported that yeast hydrolysate (YH) was effective in promoting bone growth in Sprague-Dawley (SD) rats. To further clarify the mechanism of YH, the effects of YH on proliferation, differentiation and gene expression in vitro were investigated using osteoblastic cell lines (MC3T3-E1). Cell proliferation increased significantly as much as 110% of the basal value when cells were treated with 100 µg/mL of YH. Alkaline phosphatase (ALP) activity increased significantly with a YH concentration of 25-100 µg/mL, and the activity increased 152% that of the control at 100 µg/mL. The calcium content increased as much as 129% at 100 µg/mL YH. The gene expression levels of ALP and collagen type II (COL II) significantly increased approximately 1.3-fold and 1.7-fold of control, respectively, at 100 µg/mL. YH increased significantly the mRNA level of bone sialoprotein (BSP) but not in a dose-dependent manner. The mRNA levels of bone morphogenetic proteins (BMP)-2, BMP-4, collagen type I (COL I) and osteonectin (ON) did not increase. In summary, YH increased the proliferation of osteoblasts and directly stimulated ALP and bone matrix proteins (e.g. BSP, COL II), and these increases trigger osteoblastic differentiation (e.g. mineralized nodule formation).
authors.affiliation: Department of Food and Nutrition, Korea University, Seoul, Korea.
authors.forename: Hyun-Sun
authors.initials: HS
authors.lastname: Lee
authors.forename: Eun-Young
authors.initials: EY
authors.lastname: Jung
authors.forename: Song Hwan
authors.initials: SH
authors.lastname: Bae
authors.forename: Ki Han
authors.initials: KH
authors.lastname: Kwon
authors.forename: Jin-Man
authors.initials: JM
authors.lastname: Kim
authors.forename: Hyung Joo
authors.initials: HJ
authors.lastname: Suh
mesh_terms: D016475:3T3 Cells; D000469:Alkaline Phosphatase / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D000818:Animals; D000943:Antigens, Differentiation / Q000235:genetics / Q000378:metabolism; D001846:Bone Development / Q000187:drug effects*; D002113:Calcification, Physiologic / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D024043:Collagen Type II / Q000235:genetics / Q000378:metabolism; D045424:Complex Mixtures / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005109:Extracellular Matrix / Q000187:drug effects; D005656:Fungal Proteins / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects; D058574:Integrin-Binding Sialoprotein / Q000235:genetics / Q000378:metabolism; D051379:Mice; D010006:Osteoblasts / Q000187:drug effects* / Q000502:physiology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000737:chemistry*
chemical_list: D000943:Antigens, Differentiation; D024043:Collagen Type II; D045424:Complex Mixtures; D005656:Fungal Proteins; D058574:Integrin-Binding Sialoprotein; D012333:RNA, Messenger; D000469:Alkaline Phosphatase