title: Synthesis and properties of small interfering RNA duplexes carrying 5-ethyluridine residues.
abstract: Oligoribonucleotides carrying 5-ethyluridine units were prepared using solid-phase phosphoramidite chemistry. The introduction of the tert-butyldimethylsilyl group at the 2'-OH position proceeded in good yield and very high 2'-regioselectivity. RNA duplexes carrying 5-ethyluridine either at the sense or the guide strands display RNAi activity comparable to or slightly better than that of unmodified RNA duplexes. Gene suppression experiments using luciferase targets in SH-SY5Y cells show that the ethyl group is generally well accepted at all positions although a small decrease in RNA interference activity is observed when one 5-ethylU residue is incorporated in the 3' overhangs.
authors.affiliation: Institute for Research in Biomedicine (IRB Barcelona), Institute for Advanced Chemistry of Catalonia (IQAC-CSIC), Networking Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Baldiri i Reixac 10, 08028 Barcelona, Spain.
authors.forename: Montserrat
authors.initials: M
authors.lastname: Terrazas
authors.forename: Ramon
authors.initials: R
authors.lastname: Eritja
mesh_terms: D045744:Cell Line, Tumor; D006801:Humans; D009843:Oligoribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D009943:Organophosphorus Compounds / Q000737:chemistry; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D014498:Uracil / Q000031:analogs & derivatives / Q000737:chemistry; D014529:Uridine / Q000031:analogs & derivatives* / Q000737:chemistry
chemical_list: D009843:Oligoribonucleotides; D009943:Organophosphorus Compounds; D034741:RNA, Small Interfering; C434331:phosphoramidite; C048011:5-ethyluracil; D014498:Uracil; D014529:Uridine