title: SESAME (SEquence Sorter & AMplicon Explorer): genotyping based on high-throughput multiplex amplicon sequencing.
abstract: CONCLUSIONS
  Characterizing genetic diversity through genotyping short amplicons is central to evolutionary biology. Next-generation sequencing (NGS) technologies changed the scale at which these type of data are acquired. SESAME is a web application package that assists genotyping of multiplexed individuals for several markers based on NGS amplicon sequencing. It automatically assigns reads to loci and individuals, corrects reads if standard samples are available and provides an intuitive graphical user interface (GUI) for allele validation based on the sequences and associated decision-making tools. The aim of SESAME is to help allele identification among a large number of sequences.
  
  
  BACKGROUND
  SESAME and its documentation are freely available under the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported Licence for Windows and Linux from http://www1.montpellier.inra.fr/CBGP/NGS/ or http://tinyurl.com/ngs-sesame.
authors.affiliation: Aix-Marseille Université, CNRS, IRD, UMR 6116-IMEP, Equipe Evolution, Génome et Environnement, Marseille, France. emese.meglecz@univ-provence.fr
authors.forename: Emese
authors.initials: E
authors.lastname: Meglécz
authors.forename: Sylvain
authors.initials: S
authors.lastname: Piry
authors.forename: Erick
authors.initials: E
authors.lastname: Desmarais
authors.forename: Maxime
authors.initials: M
authors.lastname: Galan
authors.forename: André
authors.initials: A
authors.lastname: Gilles
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Guivier
authors.forename: Nicolas
authors.initials: N
authors.lastname: Pech
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Martin
mesh_terms: D000483:Alleles; D005838:Genotype; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D020407:Internet; D017422:Sequence Analysis, DNA / Q000379:methods*; D012984:Software