title: Presence of drug resistance mutations among drug-naive patients in Morocco.
abstract: The aim of the present study was to determine viral subtypes and resistance mutations to antiretroviral treatment (ART) in HIV-1-infected treatment-naive patients from Rabat, Morocco during the period 2005-2009. The protease and reverse transcriptase (RT) genes were sequenced, the phylogenetic trees were inferred, and the resistance-associated mutations to NRTIs, NNRTIs, and PIs were recorded according to the international list of surveillance drug resistance mutations (SDRMs). The viral subtypes were subtype B (74%), CRF02_AG (15%), A1 (6%), C (2%), F1 (1%), CRF09 (1%), and CRF25_cpx (1%). The presence of DRMs was found in four (5.06%) of 91 patients; resistance mutations to NRTIs were M184V and T215I/S revertant mutations; resistance to NNRTIs was associated with K103N and resistance to PIs with V82A. These findings have relevant implications for the local molecular mapping of HIV-1 and future ART surveillance studies in the region.
authors.affiliation: Hôpital Militaire d'Instruction Mohammed V, Faculté de Médecine et de Pharmacie de Rabat, Morocco.
authors.forename: Hicham El
authors.initials: HE
authors.lastname: Annaz
authors.forename: Patricia
authors.initials: P
authors.lastname: Recordon-Pinson
authors.forename: Nabil
authors.initials: N
authors.lastname: Baba
authors.forename: Omar
authors.initials: O
authors.lastname: Sedrati
authors.forename: Saad
authors.initials: S
authors.lastname: Mrani
authors.forename: Hervé
authors.initials: H
authors.lastname: Fleury
mesh_terms: D000328:Adult; D019380:Anti-HIV Agents / Q000008:administration & dosage / Q000627:therapeutic use; D023241:Antiretroviral Therapy, Highly Active; D016172:DNA Fingerprinting; D024882:Drug Resistance, Viral / Q000235:genetics*; D005260:Female; D005838:Genotype; D015658:HIV Infections / Q000188:drug therapy / Q000453:epidemiology / Q000821:virology*; D016333:HIV Protease / Q000235:genetics*; D054303:HIV Reverse Transcriptase / Q000235:genetics*; D015497:HIV-1 / Q000145:classification / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification; D006801:Humans; D008875:Middle Aged; D009018:Morocco; D009154:Mutation; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012367:RNA, Viral; D012189:Retrospective Studies; D017422:Sequence Analysis, DNA; D019562:Viral Load
chemical_list: D019380:Anti-HIV Agents; D012367:RNA, Viral; D054303:HIV Reverse Transcriptase; D016333:HIV Protease