title: [Identification by PCR and antifungal susceptibility of vaginal clinical Candida sp isolates].
abstract: BACKGROUND
  Vaginal candidiasis is a condition that affects innumerous fertile women. Candida albicans is the most frequently isolated species from vaginal discharge; however, other different species that are more resistant to antifungal drugs can be identified in vaginal clinical samples.
  
  
  METHODS
  The species of 30 vaginal Candida isolates was identified by PCR using the universal ITS4 primer and species-specific primers for C. albicans, C. glabrata, C. tropicalis and C. krusei. The sensitivity pattern to amphotericin B, fluconazole and voriconazole was assessed using the CLSI M27-A2 macrodilution method.
  
  
  RESULTS
  The PCR assay revealed 28 C. albicans and 2 samples showed amplification for C. albicans and C. glabrata primers. The minimum inhibitory concentration for amphotericin B ranged from 0.03 µg/mL to 0.25 µg/mL, for fluconazole from 0.125 µg/ml to 16 µg/mL and for voriconazole from 0.03 µg/mL to 0.25 µg/m.
  
  
  CONCLUSIONS
  Identification at Candida species level by PCR assay could be relevant for clinical management of these infections.
authors.affiliation: Curso de Farmácia Bioquímica Clínica, Universidade Regional Integrada do Alto Uruguai e das Missões, Santo Ângelo, RS. izabelalmeida@hotmail.com
authors.forename: Izabel Almeida
authors.initials: IA
authors.lastname: Alves
authors.forename: Fernanda Pês de
authors.initials: FP
authors.lastname: Camargo
authors.forename: Letícia Silveira
authors.initials: LS
authors.lastname: Goulart
mesh_terms: D000666:Amphotericin B / Q000494:pharmacology; D000935:Antifungal Agents / Q000494:pharmacology*; D002175:Candida / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D002181:Candidiasis, Vulvovaginal / Q000382:microbiology*; D004271:DNA, Fungal / Q000032:analysis; D005260:Female; D015725:Fluconazole / Q000494:pharmacology; D006801:Humans; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction; D011743:Pyrimidines / Q000494:pharmacology; D014230:Triazoles / Q000494:pharmacology; D065819:Voriconazole
chemical_list: D000935:Antifungal Agents; D004271:DNA, Fungal; D011743:Pyrimidines; D014230:Triazoles; D000666:Amphotericin B; D015725:Fluconazole; D065819:Voriconazole