title: Identification of bioactive peptides in hypoallergenic infant milk formulas by capillary electrophoresis-mass spectrometry.
abstract: In this study, we use capillary electrophoresis-mass spectrometry (CE-MS) for the identification of bioactive peptides in hypoallergenic infant milk formulas (IF), which are complex bovine milk protein hydrolysates. A sample clean-up pretreatment with a citrate buffer containing dithiothreitol and urea followed by solid-phase extraction (SPE) with different reversed-phase commercial cartridges was investigated to achieve optimum detection sensitivity in CE-MS. SPE with C18, StrataX and Oasis HLB cartridges allowed detection of the largest number of low molecular mass components, but combination of C18 and StrataX results was enough to achieve an excellent coverage of the studied IF. The monoisotopic molecular mass values of the low molecular mass components obtained by capillary electrophoresis ion-trap mass spectrometry (CE-IT-MS) allowed the tentative identification of nine bioactive sequences. Only the identification of five of them could be confirmed when accurate mass measurements were performed by capillary electrophoresis time-of-flight mass spectrometry (CE-TOF-MS), namely LKP, IPY, ALPM, PGPIHN and VAGTWY, which were reported to present angiotensin-converting enzyme (ACE) inhibitory and antimicrobial activity (only VAGTWY).
authors.affiliation: Department of Analytical Chemistry, Nutrition and Food Safety Research Institute (INSA-UB), University of Barcelona, Diagonal 647, 3rd Floor, 08028 Barcelona, Spain.
authors.forename: Sergio
authors.initials: S
authors.lastname: Català-Clariana
authors.forename: Fernando
authors.initials: F
authors.lastname: Benavente
authors.forename: Estela
authors.initials: E
authors.lastname: Giménez
authors.forename: José
authors.initials: J
authors.lastname: Barbosa
authors.forename: Víctoria
authors.initials: V
authors.lastname: Sanz-Nebot
mesh_terms: D003627:Data Interpretation, Statistical; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D007223:Infant; D041943:Infant Formula / Q000737:chemistry; D007231:Infant, Newborn; D013058:Mass Spectrometry / Q000379:methods*; D015394:Molecular Structure; D010455:Peptides / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D010455:Peptides