title: An optical-parameters-free method for locating light source in deep tissue.
abstract: Bioluminescence of luciferase-expressing cells in live animals is a promising technique for image guided tumor resection. However, requiring exact optical tissue parameters is an underlying problem of diffusion equation when recovering bioluminescent images of luciferase activity within a volume using emission optical signal data from internal light sources. In this paper, an equivalence method based on the equation of radiative transfer (ERT) is proposed to locate bioluminescence source without optical tissue parameters. The solution of equivalence method is satisfied with the unique of localization as well as the complex tissue environment. The reconstructed images of bioluminescence are presented with simulated heterogeneous tissue models, and an 8.7% relative error of the source localization is obtained.
authors.affiliation: IBHE, Shenzhen Institute of Technology, Chinese Academy of Sciences, Shenzhen, China.
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Quan
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zheng
authors.forename: Yong
authors.initials: Y
authors.lastname: Yao
authors.forename: Jia
authors.initials: J
authors.lastname: Gu
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
mesh_terms: D000465:Algorithms; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008027:Light; D049449:Luminescence; D015233:Models, Statistical; D008962:Models, Theoretical; D009369:Neoplasms / Q000473:pathology; D055095:Optics and Photonics; D017785:Photons; D015203:Reproducibility of Results; D012815:Signal Processing, Computer-Assisted; D041622:Tomography, Optical / Q000379:methods*