title: [The effects of stretch sensitive positive ion channel on the high externalization of MUC5AC in airway epithelial cells by mechanical stretching].
abstract: OBJECTIVE
  The effects of stretch sensitive positive ion channel, the internal flow of Ca(2+) and myristoylated alanine-rich C kinase substrate (MARCKS) on the high externalization of MUC5AC in airway epithelial cells in mechanical stretching.
  
  
  METHODS
  Mini-type Multi-functional Bio-impact Machin was used to construct the model of mechanical stretching. The airway epithelial cells were divide into control group, stretch, stretch and gadolinium, stretch and low molecular weight heparin, stretch and nifedipine, stretch and the locked nucleic acids of MARCKS effective domain (ED), stretch and the control locked nucleic acids of MARCKS ED groups. The expression of MUC5AC and SNAP23 protein in cells were determined by immunohistochemistry method, contents of MUC5AC and SNAP23 protein were measured by Image Pro Plus 5.0. MUC5AC protein in supernatant was determined by ELISA methods. SNAP23 mRNA was determined by RT-PCR methods.
  
  
  RESULTS
  Mechanical stretching could increase the expression of SNAP23 and MUC5AC in cells and MUC5AC in supernatant (P < 0.05). Gadolinium, nifedipine and LNA of MARCKS ED could reduce the increase the expression of SNAP23 and MUC5AC in cells and MUC5AC in supernatant (all P < 0.05).
  
  
  CONCLUSIONS
  Mechanical stretching could increase the expression of MUC5AC in airway epithelial cells. This maybe is concerned with stretch sensitive positive ion channel, the internal flow of Ca(2+) and MARCKS.
authors.affiliation: Department of Respiratory Medicine, Chongqing University of Medical Science, Chongqing 400010, China.
authors.forename: Tian
authors.initials: T
authors.lastname: Zhong
authors.forename: Juliy M
authors.initials: JM
authors.lastname: Perelman
authors.forename: Victor P
authors.initials: VP
authors.lastname: Kolosov
authors.forename: Xiang-dong
authors.initials: XD
authors.lastname: Zhou
mesh_terms: D027682:Cation Transport Proteins / Q000378:metabolism*; D002460:Cell Line; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism*; D005089:Exocytosis; D006801:Humans; D055271:Mucin 5AC / Q000378:metabolism*; D009093:Mucus / Q000378:metabolism*; D010812:Physical Stimulation; D050766:Qb-SNARE Proteins / Q000378:metabolism; D050767:Qc-SNARE Proteins / Q000378:metabolism; D012137:Respiratory System / Q000166:cytology*
chemical_list: D027682:Cation Transport Proteins; C524678:MUC5AC protein, human; D055271:Mucin 5AC; D050766:Qb-SNARE Proteins; D050767:Qc-SNARE Proteins; C100493:SNAP23 protein, human