title: [Application of iTRAQ in proteomic study of prostate cancer].
abstract: Amine-specific isobaric tagging (iTRAQ) reagents, as a new class of isobaric reagent, were developed in 2004, which, combined with liquid chromatography and tandem mass spectrometry (LC-MS/MS) , have been applied to the identification and quantification of proteins in a wide range of biological samples, including bacteria, yeasts, human tissues, cells, and fluids. As a new method of quantitative proteomics, the technique of iTRAQ allows for the quantitative analysis of four samples simultaneously and displays its advantages of high-flux, food reproducibility, and high sensitivity; it also provides a potential technological platform for studying the mechanisms of the development and progression of prostate cancer.
authors.affiliation: Department of Urology, Huashan Hospital Affiliated to Fudan University, Shanghai 200040, China. drsunchuanyu@yahoo.com.cn
authors.forename: Chuan-Yu
authors.initials: CY
authors.lastname: Sun
authors.forename: Guo-Wei
authors.initials: GW
authors.lastname: Xia
authors.forename: Ke
authors.initials: K
authors.lastname: Xu
authors.forename: Qiang
authors.initials: Q
authors.lastname: Ding
mesh_terms: D006801:Humans; D007553:Isotope Labeling / Q000379:methods*; D008297:Male; D011471:Prostatic Neoplasms / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*