title: [Effect of needle-implantation at "Neiguan" (PC 6) on the expression of myocardial TGF-beta3 and its mRNA in the Chinese miniswine with myocardial ischemia injury].
abstract: OBJECTIVE
  To observe the effect of needle-implantation (NI) at "Neiguan" (PC 6) on the expression of myocardial transforming growth factor-beta 3 Protein(TGF-beta 3) and mRNA in Chinese miniswine with myocardial ischemia (MI) injury.
  
  
  METHODS
  A total of 32 Chinese Guizhou miniswine were randomly and equally divided into Sham-operation group, model group, NI-PC 6 group and NI-Geshu (BL17, NI-BL17) group. MI injury model was established by occlusion of the descending anterior branch of the left coronary artery. Two acupuncture needles (veterinary use) were separately and subcutaneously implanted into "Neiguan" (PC 6) and "Geshu" (BL17) areas for 7 days. TGF-beta 3 protein and mRNA expressions were determined by Western blot and real-time PCR techniques, separately.
  
  
  RESULTS
  In comparison with the sham-operation (sham) group, TGF-beta 3 protein and mRNA expressions in model group were upregulated significantly (P < 0.05). While compared with the model group, myocardial TGF-beta3 mRNA expression was upregulated considerably in NI-PC 6 group (P < 0.01), rather than in NI-BL17 group (P > 0.05), and myocardial TGF-beta 3 expression in both NI-PC 6 and NI-BL17 groups was upregulated obviously (P < 0.01). Comparison between NI-PC 6 and NI-BL17 groups showed that the expression levels of myocardial TGF-beta 3 protein and mRNA were significantly higher in NI-PC 6 group than in NI-BL17 group (P < 0.05).
  
  
  CONCLUSIONS
  Needle-implantation of "Neiguan" (PC 6) can upregulate myocardial TGF-beta 3 protein and mRNA expression in MI Chinese miniswine, which may contribute to its effect in improving the ischemic myocardial injury by way of enhancing the angiopoiesis.
authors.affiliation: Department of Acu-moxibustion and Massage, Guiyang College of Chinese Medicine, Guiyang 550002, China. yangxiaofang210@163.com
authors.forename: Xiao-Fang
authors.initials: XF
authors.lastname: Yang
authors.forename: Jin
authors.initials: J
authors.lastname: Cui
authors.forename: Xiao-Yu
authors.initials: XY
authors.lastname: Liu
authors.forename: Xiao-Shan
authors.initials: XS
authors.lastname: Zhang
authors.forename: Lin
authors.initials: L
authors.lastname: Feng
authors.forename: Xing-Gui
authors.initials: XG
authors.lastname: Wang
authors.forename: Ning
authors.initials: N
authors.lastname: Qian
mesh_terms: D015669:Acupuncture Points; D015670:Acupuncture Therapy; D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D015870:Gene Expression; D006801:Humans; D008297:Male; D017202:Myocardial Ischemia / Q000235:genetics* / Q000378:metabolism / Q000628:therapy*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011897:Random Allocation; D034421:Sus scrofa; D053782:Transforming Growth Factor beta3 / Q000235:genetics* / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D053782:Transforming Growth Factor beta3