title: Micro open-sandwich ELISA to rapidly evaluate thyroid hormone concentration from serum samples.
abstract: BACKGROUND
  Thyroxine (T4) is the most commonly measured thyroid hormone for the diagnosis of thyroid function. To elucidate a rapid and sensitive assay for T4, we made a microfluidics-based noncompetitive immunodetection chip system using anti-T4 antibody fragments obtained from a phage display library.
  
  
  RESULTS
  Based on the open-sandwich ELISA principle that detects antigen-dependency of the interaction between the two antibody variable regions V(H) and V(L), we could detect less than 1 ng/ml of T4. The assay was also successfully applied to evaluate total T4 concentration in the serum of healthy individuals.
  
  
  CONCLUSIONS
  This would be the first micro open-sandwich ELISA constructed with antibody fragments directly selected from immunized mice. The system will be applied to the sensitive detection of many diagnostic markers.
authors.affiliation: Department of Chemistry & Biotechnology, University of Tokyo, Tokyo, Japan.
authors.forename: Kamrun Nahar
authors.initials: KN
authors.lastname: Islam
authors.forename: Masaki
authors.initials: M
authors.lastname: Ihara
authors.forename: Jinhua
authors.initials: J
authors.lastname: Dong
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Kasagi
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Mori
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ueda
mesh_terms: D053000:Analytic Sample Preparation Methods; D000818:Animals; D001774:Blood Chemical Analysis / Q000295:instrumentation*; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation* / Q000379:methods*; D006801:Humans; D007128:Immunoglobulin Fragments / Q000276:immunology; D008297:Male; D051379:Mice; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D019151:Peptide Library; D013974:Thyroxine / Q000097:blood* / Q000276:immunology; D013997:Time Factors
chemical_list: D007128:Immunoglobulin Fragments; D019151:Peptide Library; D013974:Thyroxine