title: Visualizing resonance energy transfer in supramolecular surface patterns of β-CD-functionalized quantum dot hosts and organic dye guests by fluorescence lifetime imaging.
abstract: Detection of an analyte via supramolecular host-guest binding and quantum dot (QD)-based fluorescence resonance energy transfer (FRET) signal transduction mechanism is demonstrated. Surface patterns consisting of CdSe/ZnS QDs functionalized at their periphery with β-cyclodextrin (β-CD) were obtained by immobilization of the QDs from solution onto glass substrates patterned with adamantyl-terminated poly(propylene imine) dendrimeric "glue." Subsequent formation of host-guest complexes between vacant β-CD on the QD surface and an adamantyl-functionalized lissamine rhodamine resulting in FRET was confirmed by fluorescence microscopy, spectroscopy, and fluorescence lifetime imaging microscopy (FLIM).
authors.affiliation: Materials Science and Technology of Polymers, Faculty of Science and Technology and MESA+ Institute for Nanotechnology, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands.
authors.forename: Denis
authors.initials: D
authors.lastname: Dorokhin
authors.forename: Shu-Han
authors.initials: SH
authors.lastname: Hsu
authors.forename: Nikodem
authors.initials: N
authors.lastname: Tomczak
authors.forename: Christian
authors.initials: C
authors.lastname: Blum
authors.forename: Vinod
authors.initials: V
authors.lastname: Subramaniam
authors.forename: Jurriaan
authors.initials: J
authors.lastname: Huskens
authors.forename: David N
authors.initials: DN
authors.lastname: Reinhoudt
authors.forename: Aldrik H
authors.initials: AH
authors.lastname: Velders
authors.forename: G Julius
authors.initials: GJ
authors.lastname: Vancso
mesh_terms: D031541:Fluorescence Resonance Energy Transfer / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods*; D045663:Quantum Dots; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D047392:beta-Cyclodextrins; C031215:betadex