title: Inhibition of poly (ADP-ribose) polymerase-1 enhances doxorubicin activity against liver cancer cells.
abstract: The purpose of this study was to investigate whether PARP-1 inhibition sensitizes human liver cancer cell lines to doxorubicin treatment. Both the addition of PARP-1 inhibitor (ANI) and depletion by means of stable siRNA significantly enhanced the growth inhibition induced by the DNA damage agents used. This effect was associated with an accumulation of unrepaired DNA, with a reduction in EGFR and Bcl-xL gene expression as well as with positive annexin-V staining. These results provide novel evidence of the direct role of PARP-1 in tumour chemoresistance in relation to its effects on the transcription of key genes involved in tumour survival.
authors.affiliation: Hospital Universitario San Cecilio, Granada, Spain. jamg@ugr.es
authors.forename: J A
authors.initials: JA
authors.lastname: Muñoz-Gámez
authors.forename: R
authors.initials: R
authors.lastname: Quiles-Pérez
authors.forename: A
authors.initials: A
authors.lastname: Ruiz-Extremera
authors.forename: A B
authors.initials: AB
authors.lastname: Martín-Álvarez
authors.forename: Laura
authors.initials: L
authors.lastname: Sanjuan-Nuñez
authors.forename: A
authors.initials: A
authors.lastname: Carazo
authors.forename: Josefa
authors.initials: J
authors.lastname: León
authors.forename: F J
authors.initials: FJ
authors.lastname: Oliver
authors.forename: J
authors.initials: J
authors.lastname: Salmerón
mesh_terms: D015057:1-Naphthylamine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004260:DNA Repair / Q000187:drug effects; D004317:Doxorubicin / Q000494:pharmacology*; D004357:Drug Synergism; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D066246:ErbB Receptors / Q000032:analysis; D006801:Humans; D008113:Liver Neoplasms / Q000188:drug therapy* / Q000473:pathology; D053644:Naphthalimides / Q000494:pharmacology*; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D015363:Quinolones / Q000494:pharmacology*; D051020:bcl-X Protein / Q000032:analysis
chemical_list: D000903:Antibiotics, Antineoplastic; C494811:BCL2L1 protein, human; D004791:Enzyme Inhibitors; D053644:Naphthalimides; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D015363:Quinolones; D051020:bcl-X Protein; C086538:4-amino-1,8-naphthalimide; D004317:Doxorubicin; D015057:1-Naphthylamine; D066246:ErbB Receptors