title: Five-minute Giemsa stain for rapid detection of malaria parasites in blood smears.
abstract: The Giemsa stain is used as the gold standard for the diagnosis of malaria on blood smears. The classical staining procedure requires between 30 and 45 min. We modified the Giemsa stain and reduced the staining time to 5 min without any loss of quality.
authors.affiliation: Department of Medical Microbiology and Infection Control, VU University Medical Center, De Boelelaan 1117, Amsterdam 1081 HV, The Netherlands. m.jager@vumc.nl
authors.forename: M M
authors.initials: MM
authors.lastname: Jager
authors.forename: J L
authors.initials: JL
authors.lastname: Murk
authors.forename: R D
authors.initials: RD
authors.lastname: Piqué
authors.forename: T A M
authors.initials: TA
authors.lastname: Hekker
authors.forename: C M J E
authors.initials: CM
authors.lastname: Vandenbroucke-Grauls
mesh_terms: D000818:Animals; D001399:Azure Stains; D001800:Blood Specimen Collection / Q000379:methods; D004396:Coloring Agents; D006801:Humans; D008288:Malaria / Q000097:blood / Q000175:diagnosis* / Q000469:parasitology; D018512:Parasitemia / Q000097:blood / Q000175:diagnosis / Q000469:parasitology; D010963:Plasmodium falciparum / Q000302:isolation & purification*; D041122:Plasmodium ovale / Q000302:isolation & purification*; D010966:Plasmodium vivax / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D013194:Staining and Labeling / Q000379:methods; D013997:Time Factors
chemical_list: D001399:Azure Stains; D004396:Coloring Agents