title: Molecular packing of functionalized fluorinated lipids in Langmuir monolayers.
abstract: Fluorinated amphipaths are a fascinating class of compounds, which, despite significant challenges associated with their syntheses, have found use across a number of areas of biotechnology. Applications range from the in vitro stabilization of membrane proteins to the development of enhanced stability intravenous drug and gene delivery systems. More recently, monolayer-forming fluorinated lipids have found use in the 2D crystallization of detergent-solubilized hydrophobic or partially hydrophobic proteins at the air-water interface. In this study, we investigate the surface properties of a novel suite of monolayer forming, partially fluorinated lipids. These modular lipid structures contain a densely fluorinated insertion in the hydrocarbon tail and a synthetically modifiable headgroup. Analyses of surface-pressure area isotherms and X-ray reflectometry profiles reveal that the lipids spread into fluid monolayers and are more compressible than their non-fluorinated counterparts. Furthermore, the data support a model whereby the partially fluorinated chains of the lipid tails form a film which is fundamentally incompatible with detergents and other destabilizing amphipaths.
authors.affiliation: Institute for Molecular Bioscience, University of Queensland, St. Lucia, Queensland 4072, Australia.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Landsberg
authors.forename: Jeremy L
authors.initials: JL
authors.lastname: Ruggles
authors.forename: Waleed M
authors.initials: WM
authors.lastname: Hussein
authors.forename: Ross P
authors.initials: RP
authors.lastname: McGeary
authors.forename: Ian R
authors.initials: IR
authors.lastname: Gentle
authors.forename: Ben
authors.initials: B
authors.lastname: Hankamer
mesh_terms: D003460:Crystallization; D003902:Detergents / Q000737:chemistry; D054879:Halogenation; D050356:Lipid Metabolism; D008055:Lipids / Q000737:chemistry*; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism; D012995:Solubility; D013499:Surface Properties
chemical_list: D003902:Detergents; D008055:Lipids; D008565:Membrane Proteins