title: [Expression of the antimicrobial peptide beta-defensin-2 in the conjunctival epithelial cells in primary open-angle glaucoma and over time in the postoperative period].
abstract: The time course of changes in the postoperative expression of the antimicrobial peptide beta-defensin-2 (HBD-2) in the conjunctival epithelial cells was studied in 13 patients (13 eyes) with primary open-angle glaucoma (POAG) and 9 individuals (9 eyes) without ophthalmopathology. Scrapes from the eyelid conjunctiva and transient folds were used as a material for an immunological study. In the study group, the scrapes were taken a few hours before and at 7, 30, and 90 days after surgery. The expression of the HBD-2 gene in the study material was determined by real-time polymerase chain reaction. In the subjects without pathology of the organ of vision, the conjunctival epithelial cells exhibited HBD-2; in the patients with POAG, the baseline HBD-2 gene expression was significantly higher. The baseline HBD-2 expression did not depend on gender, the duration of local antihypertensive therapy, the stage of disease stage, and the level of ophthalmotonus. HBD-2 gene expression was by 5-10 times higher than that preoperatively in all the patients with POAG on postoperative day 7. One month after surgery, the HBD-2 gene expression considerably rose in 31% of the examined patients while it became normal in 38% of the patients.
authors.forename: V P
authors.initials: VP
authors.lastname: Erichev
authors.forename: L V
authors.initials: LV
authors.lastname: Gankovskaia
authors.forename: L V
authors.initials: LV
authors.lastname: Koval'chuk
authors.forename: O A
authors.initials: OA
authors.lastname: Gankovskaia
authors.forename: A E
authors.initials: AE
authors.lastname: Dugina
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D003228:Conjunctiva / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D004847:Epithelial Cells / Q000737:chemistry / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D005902:Glaucoma, Open-Angle / Q000378:metabolism* / Q000601:surgery*; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D023083:beta-Defensins / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics
chemical_list: C106641:DEFB4A protein, human; D023083:beta-Defensins