title: Identification of immunogenic consensus T-cell epitopes in globally distributed influenza-A H1N1 neuraminidase.
abstract: Antigenic drift is the ability of the swine influenza virus to undergo continuous and progressive changes in response to the host immune system. These changes dictate influenza vaccine updates annually to ensure inclusion of antigens of the most current strains. The identification of those peptides that stimulate T-cell responses, termed T-cell epitopes, is essential for the development of successful vaccines. In this study, the highly conserved and specific epitopes from neuraminidase of globally distributed H1N1 strains were predicted so that these potential vaccine candidates may escape with antigenic drift. A total of nine novel CD8(+) T-cell epitopes for MHC class-I and eight novel CD4(+) T-cell epitopes for MHC class-II alleles were proposed as novel epitope based vaccine candidates. Additionally, the epitope FSYKYGNGV was identified as a highly conserved, immunogenic and potential vaccine candidate, capable for generating both CD8(+) and CD4(+) responses.
authors.affiliation: Society for Biological Research & Rural Development, Lucknow, UP, India. shishir.bioinfo@gmail.com
authors.forename: Shishir K
authors.initials: SK
authors.lastname: Gupta
authors.forename: Mugdha
authors.initials: M
authors.lastname: Srivastava
authors.forename: Bashir A
authors.initials: BA
authors.lastname: Akhoon
authors.forename: Suchi
authors.initials: S
authors.lastname: Smita
authors.forename: Ulf
authors.initials: U
authors.lastname: Schmitz
authors.forename: Olaf
authors.initials: O
authors.lastname: Wolkenhauer
authors.forename: Julio
authors.initials: J
authors.lastname: Vera
authors.forename: Shailendra K
authors.initials: SK
authors.lastname: Gupta
mesh_terms: D000595:Amino Acid Sequence; D000940:Antigenic Variation; D000956:Antigens, Viral / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D018984:Epitopes, T-Lymphocyte / Q000737:chemistry / Q000276:immunology*; D040961:Genetic Drift; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000201:enzymology / Q000235:genetics / Q000276:immunology*; D007252:Influenza Vaccines / Q000276:immunology*; D007251:Influenza, Human / Q000276:immunology / Q000517:prevention & control / Q000821:virology; D009154:Mutation; D009439:Neuraminidase / Q000737:chemistry / Q000276:immunology*
chemical_list: D000956:Antigens, Viral; D018984:Epitopes, T-Lymphocyte; D007252:Influenza Vaccines; D009439:Neuraminidase