title: Expressed gene sequences of two variants of sheep interleukin-25.
abstract: This report describes the cloning and characterization of sheep interleukin-25 (IL25) expressed gene sequences and shows that, like humans, sheep express two transcript variants of IL25. Transcript variant 1 (IL25v1) has a 510 bp open reading frame encoding a 169 amino acid polypeptide with a calculated M(r) 19,200. The 498 bp IL25v2 encodes a 165 amino acid polypeptide with a calculated M(r) 18,710; both with an isoelectric point equal to 8.0307. The additional 12 bp of IL-25 isoform 1 are at the 5' end and encode an MYQA peptide, otherwise their sequences are identical. Phylogenetic analysis shows that both sheep IL-25 isoforms are most closely related to cattle and pig IL-25.
authors.affiliation: The Roslin Institute, Royal (Dick) School of Veterinary Studies, University of Edinburgh, Summerhall, Edinburgh, EH9 1QH, UK.
authors.forename: Anton
authors.initials: A
authors.lastname: Gossner
authors.forename: Anna
authors.initials: A
authors.lastname: Peers
authors.forename: Virginia
authors.initials: V
authors.lastname: Venturina
authors.forename: John
authors.initials: J
authors.lastname: Hopkins
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005786:Gene Expression Regulation / Q000502:physiology*; D014644:Genetic Variation; D020381:Interleukin-17 / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D020033:Protein Isoforms; D012756:Sheep / Q000378:metabolism*
chemical_list: D020381:Interleukin-17; D020033:Protein Isoforms