title: Transgene design.
abstract: Transgenics are powerful mouse models to understand the biological functions of genes. This chapter gives a short overview of the requirements and considerations in designing a transgene. In addition, potential important choices that have to be made in advance for the successful designing and generating a transgenic mouse model are discussed. Methods for DNA purification for microinjection are also provided in this chapter.
authors.affiliation: Department of Pathology & Medical Biology, Medical Biology Section, Molecular Genetics, University Medical Center Groningen, University of Gronignen, Groningen, The Netherlands.
authors.forename: Bart
authors.initials: B
authors.lastname: van de Sluis
authors.forename: Jan Willem
authors.initials: JW
authors.lastname: Voncken
mesh_terms: D000818:Animals; D051379:Mice; D008822:Mice, Transgenic; D008845:Microinjections / Q000379:methods; D008957:Models, Genetic; D011401:Promoter Regions, Genetic / Q000235:genetics; D019076:Transgenes / Q000235:genetics*