title: A selective NMR method for detecting choline containing compounds in liver tissue: the 1H-14N HSQC experiment.
abstract: The feasibility of a (1)H-(14)N HSQC experiment on tissues is demonstrated with a mouse liver based on the J couplings between the protons and the quadrupolar nucleus (14)N in choline. Free choline, phosphocholine, and glycerolphosphocholine (1)H-(14)N HSQC signals were selectively observed with all unwanted signals cleanly suppressed. The CH2O signals were well resolved in the two-dimensional spectrum, which can be used for quantitative analyses.
authors.affiliation: State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Institute of Physics and Mathematics, The Chinese Academy of Sciences, Wuhan 430071, China.
authors.forename: Jiezhen
authors.initials: J
authors.lastname: Mao
authors.forename: Ling
authors.initials: L
authors.lastname: Jiang
authors.forename: Bin
authors.initials: B
authors.lastname: Jiang
authors.forename: Maili
authors.initials: M
authors.lastname: Liu
authors.forename: Xi-an
authors.initials: XA
authors.lastname: Mao
mesh_terms: D000818:Animals; D002794:Choline / Q000031:analogs & derivatives* / Q000032:analysis*; D008099:Liver / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D051379:Mice; D010767:Phosphorylcholine / Q000032:analysis*
chemical_list: D010767:Phosphorylcholine; D002794:Choline