title: Suppressed primary osteoblast functions on nanoporous titania surface.
abstract: Titiania nanotubes have large potential in medical implant applications but their tissue compatibility is still controversial. Considering that the biological behavior of primary osteoblasts is closer to the in vivo situation than other common cell lines, we investigate the response of primary osteoblasts on anodized nanotextured titania surfaces. Two nanotextured surface morphologies, namely the 5 V anodized surface with a pore diameter of 25 nm and the 20 V anodized surface with a tube diameter of 80 nm are chosen for this study. Initial cell adhesion is not obviously affected by the anodized surfaces. With the exception of slightly higher intracellular alkaline phosphatase activity and more extracellular matrix deposition, cell growth, and cell differentiation represented by the expressions of osteogenesis-related genes are impaired on both anodized surfaces. This may be attributed to the compromised focal contact formation on the anodized surfaces. The difference in the phenotypes of the primary osteoblasts and the osteoblastic cell lines may partly account for the controversy in osteoblast cytocompatibility on titania nanotubes.
authors.affiliation: Department of Periodontology and Oral Medicine, School of Stomatology, The Fourth Military Medical University, Xi'an, China.
authors.forename: Lingzhou
authors.initials: L
authors.lastname: Zhao
authors.forename: Shenglin
authors.initials: S
authors.lastname: Mei
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Paul K
authors.initials: PK
authors.lastname: Chu
authors.forename: Yumei
authors.initials: Y
authors.lastname: Zhang
authors.forename: Zhifen
authors.initials: Z
authors.lastname: Wu
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002470:Cell Survival; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D005109:Extracellular Matrix / Q000378:metabolism; D015870:Gene Expression; D008422:Materials Testing; D043942:Nanotubes / Q000737:chemistry*; D010006:Osteoblasts / Q000166:cytology / Q000502:physiology*; D010012:Osteogenesis / Q000502:physiology; D016062:Porosity; D051381:Rats; D017207:Rats, Sprague-Dawley; D013499:Surface Properties; D014025:Titanium
chemical_list: D020099:Coated Materials, Biocompatible; C009495:titanium dioxide; D014025:Titanium