title: [Effects of S-layer proteins from lactobacillus against Salmonella typhimurium adhesion and invasion on Caco-2 cells].
abstract: OBJECTIVE
  S-layer proteins of Lactobacillus acidophilus were extracted and purified, then the effect of Lactobacillus acidophilus and its S-layer proteins against the adhesion and invasion of salmonella typhimurium to caco-2 cells were investigated.
  
  
  METHODS
  S-layer proteins were purified by anion-exchange column {diethylaminoethyl (DEAE) DE52}, and the inhibition of Lactobacillus acidophilus and its S-layer proteins were studied against the adhesion and invasion of salmonella typhimurium on Caco-2 cells.
  
  
  RESULTS
  S-layer proteins exhibited strongly inhibitory effects of adhesive and invading properties of Salmonella typhimurium. In the adhesive experiments (competitive, exclusive and displacement), Salmonella typhimurium adhesion was reduced by S-layer proteins and the ability of adherence to Caco-2 cells were 1.17% +/- 5.97%, 8.71% +/- 1.36% and 10.56% +/- 0.92%, respectively (P < 0.01). The influence to inhibit the competitive adhesion of Salmonella typhimurium was optimal. Furthermore, the S-layer proteins showed a stronger effect than Lactobacillus acidophilus to inhibit Salmonella typhimurium adhesion on Caco-2 monolayers (P < 0.01). Moreover, invasion of Salmonella typhimurium to Caco-2 monolayers was inhibited by S-layer proteins.
  
  
  CONCLUSIONS
  S-layer proteins inhibited adherence and invasion of Salmonella typhimurium. The result can merit a highlight for preventive or probiotic therapy in human or animals with disease caused by Salmonella typhimurium.
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China. lipengcheng305@163.com
authors.forename: Pengcheng
authors.initials: P
authors.lastname: Li
authors.forename: Xiaolan
authors.initials: X
authors.lastname: Ye
authors.forename: Zhisheng
authors.initials: Z
authors.lastname: Wang
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Yu
authors.forename: Qiang
authors.initials: Q
authors.lastname: Yang
mesh_terms: D018938:Caco-2 Cells; D006801:Humans; D007778:Lactobacillus / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000302:isolation & purification / Q000494:pharmacology*; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000254:growth & development*
chemical_list: D008562:Membrane Glycoproteins; C055764:S-layer proteins