title: The laser driven short-term heating balloon catheter: Relation between the chronic neointimal hyperplasia formation and thermal damage to arterial smooth muscle cells.
abstract: We proposed a novel laser-driven short-term heating angioplasty to realize restenosis-suppressive angioplasty for peripheral artery disease. In this study, we investigated the chronic intimal hyperplasia formation after the short-term heating dilatation in vivo, as well as the thermal damage calculation on arterial smooth muscle cells (SMCs). The prototype short-term heating balloon catheter with 5.0, 5.5, 6.0 mm φ in balloon diameter and 25 mm in balloon length were employed. The short-term heating dilatation was performed in porcine iliac arteries with dilatation conditions of 75°C (N=4) and 65°C (N=5) as peak balloon temperature, 18 ± 4s as heating duration, 3.5 atm as balloon dilatation pressure. Four weeks after the balloon dilatation, the balloon-dilated artery segments were extracted and were stained with HE and picrosirius red for histological observation. In the case of 75°C as the peak balloon temperature, neointimal hyperplasia formation was significantly reduced. In this case, the SMCs density in the artery media measured from the HE-stained specimen was 20% lower than that in the reference artery. According to the thermal damage calculation, it was estimated that the SMCs lethality in artery media after the short-term heating angioplasty was 20% in the case of 75°C as the peak balloon temperature. We demonstrated that the short-term heating dilatation reduced the number of SMCs in artery media. We think this SMCs reduction might contribute to the suppression of chronic neointimal hyperplasia.
authors.affiliation: Graduate School of Fundamental Science and Technology, Keio University (Japan). shima_natsu@arai.appi.keiro.ac.jp
authors.forename: Natsumi
authors.initials: N
authors.lastname: Shimazaki
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Hayashi
authors.forename: Mie
authors.initials: M
authors.lastname: Kunio
authors.forename: Yuka
authors.initials: Y
authors.lastname: Igami
authors.forename: Tsunenori
authors.initials: T
authors.lastname: Arai
authors.forename: Masami
authors.initials: M
authors.lastname: Sakurada
mesh_terms: D000818:Animals; D002404:Catheterization / Q000295:instrumentation*; D002478:Cells, Cultured; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006979:Hyperthermia, Induced / Q000295:instrumentation*; D007834:Lasers; D032389:Myocytes, Smooth Muscle / Q000473:pathology* / Q000528:radiation effects*; D058426:Neointima / Q000473:pathology* / Q000503:physiopathology*; D051381:Rats