title: Genome-wide RNAi screens in African trypanosomes identify the nifurtimox activator NTR and the eflornithine transporter AAT6.
abstract: To be effective, therapeutic compounds must typically enter target cells and, in some cases, must be concentrated or modified. Thus, uptake and activation mechanisms often form the basis of selectivity against infectious agents. Loss-of-function screens can be used to identify proteins involved in drug uptake and metabolism and may also identify clinically relevant potential resistance mechanisms. We used a genome-scale RNA interference (RNAi) library to identify loss-of-function resistance mechanisms in bloodstream-form Trypanosoma brucei. Nifurtimox-Eflornithine Combination Therapy (NECT) was recently introduced for Human African Trypanosomiasis and we focus on these drugs here. Screens for resistance to nifurtimox and a related drug, benznidazole, identified loss of nitroreductase (NTR) pro-drug activator function. A screen for resistance to the amino-acid analogue, eflornithine, identified loss of amino-acid transporter (AAT6) function. Our results confirm recent findings and suggest that NTR or AAT6 loss-of-function represent major potential mechanisms of resistance to these drugs. Thus, bloodstream-form T. brucei RNAi libraries present a versatile tool for selective genetic screening and for the rapid identification of drug-activation, uptake and potential resistance mechanisms.
authors.affiliation: London School of Hygiene & Tropical Medicine, Keppel Street, London WC1E 7HT, UK.
authors.forename: Nicola
authors.initials: N
authors.lastname: Baker
authors.forename: Sam
authors.initials: S
authors.lastname: Alsford
authors.forename: David
authors.initials: D
authors.lastname: Horn
mesh_terms: D026905:Amino Acid Transport Systems / Q000235:genetics* / Q000378:metabolism; D004351:Drug Resistance / Q000235:genetics*; D000518:Eflornithine / Q000494:pharmacology; D055785:Gene Knockdown Techniques; D017125:Genes, Protozoan / Q000235:genetics*; D055106:Genome-Wide Association Study; D006801:Humans; D009547:Nifurtimox / Q000494:pharmacology; D009593:Nitroimidazoles / Q000494:pharmacology; D009601:Nitroreductases / Q000235:genetics* / Q000378:metabolism; D034622:RNA Interference; D014346:Trypanosoma brucei brucei / Q000187:drug effects / Q000201:enzymology / Q000235:genetics
chemical_list: D026905:Amino Acid Transport Systems; D009593:Nitroimidazoles; D009601:Nitroreductases; D009547:Nifurtimox; C009999:benzonidazole; D000518:Eflornithine