title: Properties of tryptophan indole-lyase from a piezophilic bacterium, Photobacterium profundum SS9.
abstract: Tryptophan indole-lyase (Trpase), PBPRA2532, from Photobacterium profundum SS9, a piezophilic marine bacterium, has been cloned, expressed in Escherichia coli, and purified. The P. profundum Trpase (PpTrpase) exhibits similar substrate specificity as the enzyme from E. coli (EcTrpase). PpTrpase has an optimum temperature for activity at about 30°C, compared with 53°C for EcTrpase, and loses activity rapidly (t(1/2)∼30min) when incubated at 50°C, while EcTrpase is stable up to 65°C. PpTrpase retains complete activity when incubated more than 3h at 0°C, while EcTrpase has only about 20% remaining activity. Under hydrostatic pressure, PpTrpase remains fully active up to 100MPa (986atm), while EcTrpase exhibits only about 10% activity at 100MPa. PpTrpase forms external aldimine and quinonoid intermediates in stopped-flow experiments with l-Trp, S-Et-l-Cys, S-benzyl-l-Cys, oxindolyl-l-Ala, l-Ala and l-Met, similar to EcTrpase. However, with l-Trp a gem-diamine is observed that decays to a quinonoid complex. An aminoacrylate is observed with l-Trp in the presence of benzimidazole, as was seen previously with EcTrpase [28] but not with S-Et-l-Cys. The results show that PpTrpase is adapted for optimal activity in the low temperature, high pressure marine environment.
authors.affiliation: Department of Chemistry, University of Georgia, Athens, GA 30602, USA. rsphillips@chem.uga.edu
authors.forename: Robert S
authors.initials: RS
authors.lastname: Phillips
authors.forename: Rashin
authors.initials: R
authors.lastname: Ghaffari
authors.forename: Peter
authors.initials: P
authors.lastname: Dinh
authors.forename: Santiago
authors.initials: S
authors.lastname: Lima
authors.forename: Douglas
authors.initials: D
authors.lastname: Bartlett
mesh_terms: D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D004795:Enzyme Stability; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D006874:Hydrostatic Pressure; D007700:Kinetics; D010776:Photobacterium / Q000201:enzymology* / Q000235:genetics; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013045:Species Specificity; D013053:Spectrophotometry; D013379:Substrate Specificity; D013696:Temperature; D014368:Tryptophanase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial; D011994:Recombinant Proteins; D014368:Tryptophanase