title: Enzymes of SPZ7 phage: isolation and properties.
abstract: Bacteriophage enzyme preparations exolysin and endolysin were studied. Exolysin (a phage-associated enzyme) was obtained from tail fraction and endolysin from phage-free cytoplasmic fraction of disintegrated Salmonella enteritidis cells. A new method for purification of these enzymes was developed, and their molecular masses were determined. The main catalytic properties of the studied enzymes (pH optimum and specificity to bacterial substrates) were found to be similar. Both enzymes lyse Escherichia coli cells like chicken egg lysozyme, but more efficiently lyse S. enteritidis cells and cannot lyse Micrococcus luteus, a good substrate for chicken egg lysozyme. Similar properties of exolysin and endolysin suggest that these enzymes are structurally similar or even identical.
authors.affiliation: Faculty of Chemistry, Lomonosov Moscow State University, Moscow, 119991, Russia. levashov@aport.ru
authors.forename: P A
authors.initials: PA
authors.lastname: Levashov
authors.forename: D V
authors.initials: DV
authors.lastname: Popov
authors.forename: V M
authors.initials: VM
authors.lastname: Popova
authors.forename: E L
authors.initials: EL
authors.lastname: Zhilenkov
authors.forename: O A
authors.initials: OA
authors.lastname: Morozova
authors.forename: N G
authors.initials: NG
authors.lastname: Belogurova
authors.forename: S A
authors.initials: SA
authors.lastname: Sedov
authors.forename: I A
authors.initials: IA
authors.lastname: Dyatlov
authors.forename: N L
authors.initials: NL
authors.lastname: Klyachko
authors.forename: A V
authors.initials: AV
authors.lastname: Levashov
mesh_terms: D000818:Animals; D055162:Biocatalysis; D002645:Chickens; D010450:Endopeptidases / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism; D009113:Muramidase / Q000378:metabolism; D012483:Salmonella Phages / Q000201:enzymology*; D012477:Salmonella enteritidis / Q000187:drug effects; D013379:Substrate Specificity; D014764:Viral Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D014764:Viral Proteins; D009113:Muramidase; D010450:Endopeptidases; C001143:endolysin