title: Crucial role of the influenza virus NS2 (NEP) C-terminal domain in M1 binding and nuclear export of vRNP.
abstract: The influenza virus genome replicates in the host cell nucleus, and the progeny viral ribonucleoproteins (vRNPs) are exported to the cytoplasm prior to maturation. The influenza virus NS2 protein has a nuclear export signal (NES) and binds to M1. It is therefore postulated that vRNP is exported from the nucleus by binding to NS2 through M1. However, the significance of the association between NS2 and M1 for the nuclear export of vRNP is still poorly understood. We herein demonstrate that the C-terminal domain of NS2 (residues 81-100) is essential for M1 binding and the nuclear export of progeny vRNPs.
authors.affiliation: Laboratory of Molecular Biology of Infectious Agents, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan.
authors.forename: Teppei
authors.initials: T
authors.lastname: Shimizu
authors.forename: Naoki
authors.initials: N
authors.lastname: Takizawa
authors.forename: Ken
authors.initials: K
authors.lastname: Watanabe
authors.forename: Kyosuke
authors.initials: K
authors.lastname: Nagata
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Kobayashi
mesh_terms: D021581:Active Transport, Cell Nucleus; D000595:Amino Acid Sequence; D001665:Binding Sites / Q000502:physiology; D015153:Blotting, Western; D003593:Cytoplasm / Q000378:metabolism / Q000821:virology; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D009154:Mutation; D011485:Protein Binding; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012261:Ribonucleoproteins / Q000235:genetics / Q000378:metabolism*; D014162:Transfection; D014763:Viral Matrix Proteins / Q000235:genetics / Q000378:metabolism*; D017361:Viral Nonstructural Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: C488936:M1 protein, Influenza A virus; C556232:NS2 protein, influenza virus A; D011993:Recombinant Fusion Proteins; D012261:Ribonucleoproteins; D014763:Viral Matrix Proteins; D017361:Viral Nonstructural Proteins; D049452:Green Fluorescent Proteins