title: Is BaF3 bioassay useful to identify patients with bioinactive growth hormone?
abstract: We analyzed the ability of the BaF3 cell line bioassay to select patients with biologically inactive GH. We first evaluated the biological response of the Ba/F3-hGHR cells to rhGH additional doses from 10 to 5000 pg/ml. The concentration points corresponding to the linear part of the curve were selected. We then analyzed a group of sera, diluted like the standard, including the entire range of GH concentrations that can be analyzed by bioassay. The serum/standard area below the curve ratio was calculated. Serum GH immunoactivity determined by IMMULITE/GH bioactivity ratios was calculated. Our experimental data showed that GH-bioactivity/GH-immunoactivity ratios below 0.303 are indicative of a bioinactive GH molecule. This bioassay would recognize only extreme cases of GH bioinactivity, and it would not be a useful tool in the search for patients with altered forms of GH.
authors.affiliation: Pediatric Department, University ofPavia, Italy.
authors.forename: Sara
authors.initials: S
authors.lastname: Pagani
authors.forename: Eduardo A
authors.initials: EA
authors.lastname: Chaler
authors.forename: Cristina
authors.initials: C
authors.lastname: Meazza
authors.forename: Mercedes
authors.initials: M
authors.lastname: Maceiras
authors.forename: Maria Eugenia
authors.initials: ME
authors.lastname: Gonzalez
authors.forename: Marco A
authors.initials: MA
authors.lastname: Rivarola
authors.forename: Francesca
authors.initials: F
authors.lastname: Cantoni
authors.forename: Paola
authors.initials: P
authors.lastname: Travaglino
authors.forename: Lucia
authors.initials: L
authors.lastname: Della Croce
authors.forename: Kamilia
authors.initials: K
authors.lastname: Laarej
authors.forename: Mauro
authors.initials: M
authors.lastname: Bozzola
authors.forename: Alicia
authors.initials: A
authors.lastname: Belgorosky
mesh_terms: D000293:Adolescent; D000818:Animals; D001681:Biological Assay / Q000379:methods*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002648:Child; D002675:Child, Preschool; D005260:Female; D006130:Growth Disorders / Q000097:blood* / Q000175:diagnosis; D019382:Human Growth Hormone / Q000097:blood* / Q000276:immunology / Q000494:pharmacology; D006801:Humans; D008297:Male; D051379:Mice; D011237:Predictive Value of Tests; D011994:Recombinant Proteins; D012016:Reference Values; D015203:Reproducibility of Results
chemical_list: D011994:Recombinant Proteins; D019382:Human Growth Hormone