title: Interleukin-32 gamma specific monoclonal antibody and developing IL-32 specific ELISA.
abstract: Cytokines are essential coordinators of defensive immune responses for resolving the invasion of pathogens such as bacteria, virus, and fungi. However, dysregulated cytokines are the main cause of various autoinflammatory immune disorders such as rheumatoid arthritis, inflammatory bowel disease, and psoriasis. Interleukin-32 (IL-32) is a recently described cytokine and characterized as a proinflammatory cytokine. IL-32 stimulates monocytes and macrophages to induce important proinflammatory cytokines (IL-1β, IL-6, and TNFα) and chemokines (IL-8 and MIP-2) by activating the NF-κB and p38 mitogen-activated protein (MAP) kinase pathways. The biological activities of IL-32 are associated with epidemic pathogens, Mycobacterium tuberculosis, influenza A virus, and human immunodeficiency virus (HIV). IL-32 is transcribed as six alternative splice variants (α, β, γ, δ, ɛ, and ζ), with IL-32γ being the most active isoform. However, it is unclear which isoform is related to specific disease activities since there are no high quality antibodies available to measure circulating IL-32 in biological samples of patients. Therefore, we developed specific anti-human IL-32γ monoclonal antibodies from recombinant human IL-32γ, which was expressed in Escherichia coli. The IL-32γ specific monoclonal antibodies recognized IL-32 in cell culture supernatants and serum of IL-32γ transgenic mice. The newly developed IL-32γ monoclonal antibodies will be a useful tool to measure IL-32 level in serum samples of various inflammatory diseases. These monoclonal antibodies will be helpful in investigating the precise function of IL-32 in immune responses and in autoinflammatory diseases.
authors.affiliation: Department of Immunology, College of Medicine, Konkuk University, Chungju City, Chungbuk, Korea.
authors.forename: Siyoung
authors.initials: S
authors.lastname: Lee
authors.forename: Sunjong
authors.initials: S
authors.lastname: Kim
authors.forename: Suyoung
authors.initials: S
authors.lastname: Bae
authors.forename: Jida
authors.initials: J
authors.lastname: Choi
authors.forename: Jaewoo
authors.initials: J
authors.lastname: Hong
authors.forename: Soyoon
authors.initials: S
authors.lastname: Ryoo
authors.forename: Hyunjhung
authors.initials: H
authors.lastname: Jhun
authors.forename: Kwangwon
authors.initials: K
authors.lastname: Hong
authors.forename: Eunsom
authors.initials: E
authors.lastname: Kim
authors.forename: Seunghyun
authors.initials: S
authors.lastname: Jo
authors.forename: Erk
authors.initials: E
authors.lastname: Her
authors.forename: Soohyun
authors.initials: S
authors.lastname: Kim
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D001710:Biotin; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D047468:Immunoprecipitation; D007378:Interleukins / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008822:Mice, Transgenic; D020033:Protein Isoforms / Q000235:genetics / Q000276:immunology; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; C496971:IL32 protein, human; D007378:Interleukins; D020033:Protein Isoforms; D011994:Recombinant Proteins; D001710:Biotin