title: The characterization of human adenylate kinases 7 and 8 demonstrates differences in kinetic parameters and structural organization among the family of adenylate kinase isoenzymes.
abstract: Differences in expression profiles, substrate specificities, kinetic properties and subcellular localization among the AK (adenylate kinase) isoenzymes have been shown to be important for maintaining a proper adenine nucleotide composition for many different cell functions. In the present study, human AK7 was characterized and its substrate specificity, kinetic properties and subcellular localization determined. In addition, a novel member of the human AK family, with two functional domains, was identified and characterized and assigned the name AK8. AK8 is the second known human AK with two complete and active AK domains within its polypeptide chain, a feature that has previously been shown for AK5. The full-length AK8, as well as its two domains AK8p1 and AK8p2, all showed similar AK enzyme activity. AK7, full-length AK8, AK8p1 and AK8p2 phosphorylated AMP, CMP, dAMP and dCMP with ATP as the phosphate donor, and also AMP, CMP and dCMP with GTP as the phosphate donor. Both AK7 and full-length AK8 showed highest affinity for AMP with ATP as the phosphate donor, and proved to be more efficient in AMP phosphorylation as compared with the major cytosolic isoform AK1. Expression of the proteins fused with green fluorescent protein demonstrated a cytosolic localization for both AK7 and AK8.
authors.affiliation: Department of Laboratory Medicine, Karolinska Institute, Huddinge, Sweden. Christakis.Panayiotou@ki.se
authors.forename: Christakis
authors.initials: C
authors.lastname: Panayiotou
authors.forename: Nicola
authors.initials: N
authors.lastname: Solaroli
authors.forename: Yunjian
authors.initials: Y
authors.lastname: Xu
authors.forename: Magnus
authors.initials: M
authors.lastname: Johansson
authors.forename: Anna
authors.initials: A
authors.lastname: Karlsson
mesh_terms: D000263:Adenylate Kinase / Q000737:chemistry* / Q000378:metabolism; D003600:Cytosol / Q000737:chemistry; D006801:Humans; D007527:Isoenzymes; D007700:Kinetics; D009711:Nucleotides / Q000378:metabolism; D010766:Phosphorylation; D013379:Substrate Specificity
chemical_list: D007527:Isoenzymes; D009711:Nucleotides; C556108:AK7 protein, human; C556107:AK8 protein, human; D000263:Adenylate Kinase