title: A multiplex ligation detection assay for the characterization of Salmonella enterica strains.
abstract: A proof of principle of a multi-target assay for genotyping Salmonella has been developed targeting 62 genomic marker sequences of Salmonella related to pathogenicity. The assay is based on multiplex ligation detection reaction (LDR) followed by customized ArrayTube® microarray detection. The feasibility of the developed assay was verified in a method comparison study with conventional PCR using 16 Salmonella 'test' strains comprising eight serovars. Subsequently, the feasibility of the LDR microarray assay was also tested by analyzing 41 strains belonging to 23 serovars. With the exception of four serovars each serovar was characterized by a unique virulence associated gene repertoire. The LDR microarray platform proved to be a convenient, rapid and easy to use tool with potential in tracing a Salmonella contamination in the food chain, for outbreak studies, and to provide data for risk assessors that support bio-traceability models.
authors.affiliation: RIKILT-Institute of Food Safety, Wageningen UR, Akkermaalsbos 2, 6708 WB Wageningen, the Netherlands. henk.aarts@rivm.nl
authors.forename: Henk J M
authors.initials: HJ
authors.lastname: Aarts
authors.forename: Pieter
authors.initials: P
authors.lastname: Vos
authors.forename: Jonas T
authors.initials: JT
authors.lastname: Larsson
authors.forename: Angela H A M
authors.initials: AH
authors.lastname: van Hoek
authors.forename: Stephan
authors.initials: S
authors.lastname: Huehn
authors.forename: Thijs
authors.initials: T
authors.lastname: Weijers
authors.forename: Hugo Ahlm
authors.initials: HA
authors.lastname: Grønlund
authors.forename: Burkhard
authors.initials: B
authors.lastname: Malorny
mesh_terms: D000003:Abattoirs; D000818:Animals; D005838:Genotype; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016133:Polymerase Chain Reaction; D019779:Salmonella enterica / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D013552:Swine