title: Altered mitochondrial regulation in quadriceps muscles of patients with COPD.
abstract: Evidence exists for locomotor muscle impairment in patients with chronic obstructive pulmonary disease (COPD), including fiber type alterations and reduced mitochondrial oxidative capacity. In this study high-resolution respirometry was used to quantify oxygen flux in permeabilized fibres from biopsies of the vastus lateralis muscle in patients with COPD and compared to healthy control subjects. The main findings of this study were that (i) routine state 2 respiration was higher in COPD; (ii) state 3 respiration in the presence of ADP was similar in both groups with substrate supply of electrons to complex I (COPD 38·28 ± 3·58 versus control 42·85 ± 3·10 pmol s(-1) mg tissue(-1) ), but O(2) flux with addition of succinate was lower in COPD patients (COPD 63·72 ± 6·33 versus control 95·73 ± 6·53 pmol s(-1) mg tissue(-1) ); (iii) excess capacity of cytochrome c oxidase in COPD patients was only ~50% that of control subjects. These results indicate that quadriceps muscle mitochondrial function is altered in patients with COPD. The regulatory mechanisms underlying these functional abnormalities remain to be uncovered.
authors.affiliation: Centre for Healthy Aging, Department of Biomedical Sciences, University of Copenhagen, Denmark.
authors.forename: Ashley I
authors.initials: AI
authors.lastname: Naimi
authors.forename: Jean
authors.initials: J
authors.lastname: Bourbeau
authors.forename: Helene
authors.initials: H
authors.lastname: Perrault
authors.forename: Jacinthe
authors.initials: J
authors.lastname: Baril
authors.forename: Cynthia
authors.initials: C
authors.lastname: Wright-Paradis
authors.forename: Amanda
authors.initials: A
authors.lastname: Rossi
authors.forename: Tanja
authors.initials: T
authors.lastname: Taivassalo
authors.forename: A William
authors.initials: AW
authors.lastname: Sheel
authors.forename: Rasmus
authors.initials: R
authors.lastname: Rabøl
authors.forename: Flemming
authors.initials: F
authors.lastname: Dela
authors.forename: Robert
authors.initials: R
authors.lastname: Boushel
mesh_terms: D000244:Adenosine Diphosphate / Q000378:metabolism; D000368:Aged; D001706:Biopsy; D016022:Case-Control Studies; D019069:Cell Respiration; D003718:Denmark; D045222:Electron Transport Chain Complex Proteins / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000378:metabolism; D004734:Energy Metabolism; D006801:Humans; D008168:Lung / Q000503:physiopathology; D008297:Male; D008875:Middle Aged; D008931:Mitochondria, Muscle / Q000378:metabolism*; D010101:Oxygen Consumption; D029424:Pulmonary Disease, Chronic Obstructive / Q000378:metabolism* / Q000503:physiopathology; D052097:Quadriceps Muscle / Q000378:metabolism*; D012129:Respiratory Function Tests; D019802:Succinic Acid / Q000378:metabolism
chemical_list: D045222:Electron Transport Chain Complex Proteins; D000244:Adenosine Diphosphate; D019802:Succinic Acid; D003576:Electron Transport Complex IV