title: Functional argument for the existence of an avian nitric oxide synthase in muscle mitochondria: effect of cold acclimation.
abstract: We report the first evidence of a mitochondrial NO synthase (mtNOS) in bird skeletal muscle. In vitro, mtNOS activity stimulated by L-arginine reduced intermyofibrillar mitochondrial oxygen uptake and ATP synthesis rates, stimulated endogenous H(2)O(2) generation, but had no effect on oxidative phosphorylation efficiency. Arginine-induced effects were fully reversed by L-NAME, a known NOS inhibitor. When ducklings were cold exposed for 4 weeks, muscle mitochondria displayed an increased state 3 respiration, a reduced H(2)O(2) generation but no significant alteration in mtNOS activity. We conclude that mtNOS is expressed in avian skeletal muscle.
authors.affiliation: Université de Lyon, F-69000, Laboratoire de Physiologie Intégrative, Cellulaire et Moléculaire, CNRS-UMR 5123 Université Lyon 1, Villeurbanne, France. benjamin.rey@univ-lyon1.fr
authors.forename: Benjamin
authors.initials: B
authors.lastname: Rey
authors.forename: Damien
authors.initials: D
authors.lastname: Roussel
authors.forename: Loïc
authors.initials: L
authors.lastname: Teulier
authors.forename: Pierre
authors.initials: P
authors.lastname: Eyenga
authors.forename: Cyril
authors.initials: C
authors.lastname: Degletagne
authors.forename: Maud
authors.initials: M
authors.lastname: Belouze
authors.forename: Claude
authors.initials: C
authors.lastname: Duchamp
mesh_terms: D000064:Acclimatization; D000818:Animals; D000831:Animals, Newborn; D046090:Anseriformes / Q000378:metabolism*; D001120:Arginine / Q000494:pharmacology; D030161:Avian Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D003080:Cold Temperature; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D006861:Hydrogen Peroxide / Q000378:metabolism; D008297:Male; D008931:Mitochondria, Muscle / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D018482:Muscle, Skeletal / Q000201:enzymology; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D010085:Oxidative Phosphorylation / Q000187:drug effects; D010101:Oxygen Consumption / Q000187:drug effects; D019802:Succinic Acid / Q000378:metabolism
chemical_list: D030161:Avian Proteins; D004791:Enzyme Inhibitors; D001120:Arginine; D019802:Succinic Acid; D006861:Hydrogen Peroxide; D019001:Nitric Oxide Synthase; D019331:NG-Nitroarginine Methyl Ester