title: Sendai virus induced cytoplasmic actin remodeling correlates with efficient virus particle production.
abstract: Cytoplasmic actins have been found interacting with viral proteins and identified in virus particles. We analyzed by confocal microscopy the cytoplasmic β- and γ-actin patterns during the course of Sendai virus infections in polarized cells. We observed a spectacular remodeling of the β-cytoplasmic actin which correlated with productive viral multiplication. Conversely, suppression of M during the course of a productive infection resulted in the decrease of particle production and the absence of β-actin remodeling. As concomitant suppression of β- and γ-actins resulted as well in reduction of virus particle production, we propose that Sendai virus specifically induces actin remodeling in order to promote efficient virion production. Beta- and γ-cytoplasmic actin recruitment could substitute for that of the endosomal sorting complex required for transport (ESCRT) mobilized by other enveloped viruses but apparently not used by Sendai virus.
authors.affiliation: Department of Microbiology and Molecular Medicine, Faculty of Medicine University of Geneva, CMU, 1 rue Michel-Servet, 1211 Geneva 4, Switzerland.
authors.forename: Vincent
authors.initials: V
authors.lastname: Miazza
authors.forename: Geneviève
authors.initials: G
authors.lastname: Mottet-Osman
authors.forename: Sergei
authors.initials: S
authors.lastname: Startchick
authors.forename: Christine
authors.initials: C
authors.lastname: Chaponnier
authors.forename: Laurent
authors.initials: L
authors.lastname: Roux
mesh_terms: D000199:Actins / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D003593:Cytoplasm / Q000378:metabolism*; D004285:Dogs; D015967:Gene Expression Regulation, Viral / Q000502:physiology; D018613:Microscopy, Confocal; D020033:Protein Isoforms; D034741:RNA, Small Interfering; D029082:Sendai virus / Q000502:physiology*; D014763:Viral Matrix Proteins / Q000235:genetics / Q000378:metabolism; D014771:Virion / Q000502:physiology*; D014779:Virus Replication / Q000502:physiology*
chemical_list: D000199:Actins; C097020:M protein, Sendai virus; D020033:Protein Isoforms; D034741:RNA, Small Interfering; D014763:Viral Matrix Proteins