title: Antioxidant effect of lemon verbena extracts in lymphocytes of university students performing aerobic training program.
abstract: Aerobic training is related to an increase in blood oxidation markers. The purpose of the present study was to investigate the antioxidant capacity of Lippia citriodora extracts (PLX(®) ) on plasma and blood cell oxidative status of university students beginning a 21 days aerobic training routine (3 days/week). Using a double-blind design, 15 male athletes (21 ± 2.1 years) were assigned to a group consuming 1.8 g/day of the plant extract (PLX(®) -group) or a placebo (PLB-group). Two blood extractions were performed at day 0 and 21, from which lymphocytes, erythrocytes and plasma were isolated. Several circulating parameters, antioxidant enzyme activities and oxidative stress markers were measured. The PLX(®) -group displayed an increased HDL-cholesterol, a modest decrease in erythrocyte number and an increased circulating urea. Activation of glutathione (GSH)-reductase was observed in erythrocytes and lymphocytes of PLX(®) -group, accompanied by lower levels of oxidative stress markers, such as malondialdehyde and protein carbonyls in plasma. The antioxidant action exerted by PLX(®) on GSH-reductase seems to be post-translational and mainly due to verbascoside, a phenylpropanoid that represents 10% (w/w) of extract content. In conclusion, PLX(®) shows antioxidant properties that could play an important role in modulating GSH-reductase activity in lymphocytes and erythrocytes and protecting plasma from exercise oxidative damage.
authors.affiliation: Applied Biology Department - Nutrition, University Miguel Hernandez, Elche (Alicante), Spain.
authors.forename: L
authors.initials: L
authors.lastname: Carrera-Quintanar
authors.forename: L
authors.initials: L
authors.lastname: Funes
authors.forename: E
authors.initials: E
authors.lastname: Viudes
authors.forename: J
authors.initials: J
authors.lastname: Tur
authors.forename: V
authors.initials: V
authors.lastname: Micol
authors.forename: E
authors.initials: E
authors.lastname: Roche
authors.forename: A
authors.initials: A
authors.lastname: Pons
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000494:pharmacology*; D008076:Cholesterol, HDL / Q000097:blood / Q000187:drug effects; D004311:Double-Blind Method; D004906:Erythrocyte Count; D004912:Erythrocytes / Q000187:drug effects; D015444:Exercise / Q000502:physiology*; D005081:Exercise Therapy; D005980:Glutathione Reductase / Q000097:blood / Q000187:drug effects; D006801:Humans; D032411:Lippia; D008214:Lymphocytes / Q000187:drug effects*; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D050050:Protein Carbonylation / Q000187:drug effects; D013334:Students; D014495:Universities; D014508:Urea / Q000097:blood; D055815:Young Adult
chemical_list: D000975:Antioxidants; D008076:Cholesterol, HDL; D010936:Plant Extracts; D008315:Malondialdehyde; D014508:Urea; D005980:Glutathione Reductase