title: Comparative analysis of the phenotypic characteristics of high- and low-virulent strains of Edwardsiella tarda.
abstract: Edwardsiella tarda is a causative agent of edwardsiellosis in freshwater and marine fish. Extracellular enzymic, haemolytic, hydrophobic and serum resistance activities, haemagglutination, autoagglutination and siderophores of high- and low- virulent E. tarda strains were examined. The results revealed different haemagglutination, autoagglutination, haemolytic, hydrophobic and serum resistance activities in different strains. Analysis of extracellular proteins (ECPs) and outer membrane proteins (OMPs) demonstrated several major, low molecular weight, virulent-strain-specific proteins, which could be virulence-related. Based on the database search with MALDI-TOF MS data, the closest homologies of the three protein bands Ed1, Ed2 and Ed3 were phosphotransferase enzyme family protein, nitrite reductase [NAD(P)H], large subunit and ATP-dependent Lon protease, respectively. A comparison of pathogenicity of purified lipopolysaccharide (LPS) and lipid A from virulent and avirulent strains demonstrated that LPS was one of the virulence factors of the E. tarda isolates, and lipid A was a biologically active determinant of LPS.
authors.affiliation: Department of Marine Biology, Ocean University of China, Qingdao, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.forename: X H
authors.initials: XH
authors.lastname: Zhang
authors.forename: B
authors.initials: B
authors.lastname: Austin
mesh_terms: D000818:Animals; D020609:Edwardsiella tarda / Q000201:enzymology / Q000472:pathogenicity*; D004591:Electrophoresis, Polyacrylamide Gel; D004756:Enterobacteriaceae Infections / Q000382:microbiology / Q000662:veterinary*; D005393:Fish Diseases / Q000382:microbiology*; D008050:Lipid A / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D017686:Oncorhynchus mykiss; D010641:Phenotype; D037521:Virulence Factors / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D008050:Lipid A; D008070:Lipopolysaccharides; D037521:Virulence Factors