title: Role of the shape of various bacteria in their separation by Microthermal Field-Flow Fractionation.
abstract: The steady-state movement of the spherical and non-spherical particles, such as prolate or oblate rotational ellipsoids, cylinders, or parallelepipeds, suspended in a liquid and exposed to a unidirectional temperature gradient, is analyzed theoretically. The differences in the ratios of the rotational to translational diffusion coefficients of the non-spherical to spherical particles, the heterogeneity of thermal conductivity of the particle body, and the heterogeneity in surface chemical nature make possible to separate the particles according to differences in shape. Preliminary experimental separations of Gram-positive and Gram-negative, nearly spherical and rod-shaped bacteria performed by Microthermal Field-Flow Fractionation confirmed that the fractionation of the cells according to differences in shape is possible.
authors.affiliation: Department of Physics and Materials Engineering, Faculty of Technology, Tomas Bata University in Zlín, Czech Republic. jjanca@ft.utb.cz
authors.forename: Josef
authors.initials: J
authors.lastname: Janča
authors.forename: Věra
authors.initials: V
authors.lastname: Halabalová
authors.forename: Jan
authors.initials: J
authors.lastname: Růžička
mesh_terms: D001419:Bacteria / Q000737:chemistry* / Q000302:isolation & purification; D031521:Fractionation, Field Flow / Q000379:methods*; D010316:Particle Size