title: 7,8-secolignans from Schisandra wilsoniana and their anti-HIV-1 activities.
abstract: Two new 7,8-secolignans, marphenols A and B (1 and 2, resp.), together with a known related derivative, 7,8-secoholostylone B (3), were isolated from the stems of Schisandra wilsoniana. The structures of 1 and 2 were elucidated by spectroscopic methods, including extensive 1D- and 2D-NMR techniques. The anti-HIV-1 activities of 1-3 were evaluated. Compound 1 inhibited HIV-1(IIIB)-induced syncytia formation with an EC(50) value of 0.55 μg ml(-1). It reduced p24 antigen expression in acutely HIV-1(IIIB)-infected C8166 cells and primary isolate HIV-1(TC-2)-infected peripheral blood mononuclear cells (PBMCs), with EC(50) values of 3.34 and 0.52 μg ml(-1), respectively. It showed no effects on the HIV-1(IIIB) replication in chronically infected H9 cells as well as fusion inhibition.
authors.affiliation: Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences and Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, P. R. China.
authors.forename: Xing-Jie
authors.initials: XJ
authors.lastname: Zhang
authors.forename: Guang-Yu
authors.initials: GY
authors.lastname: Yang
authors.forename: Rui-Rui
authors.initials: RR
authors.lastname: Wang
authors.forename: Jian-Xin
authors.initials: JX
authors.lastname: Pu
authors.forename: Han-Dong
authors.initials: HD
authors.lastname: Sun
authors.forename: Wei-Lie
authors.initials: WL
authors.lastname: Xiao
authors.forename: Yong-Tang
authors.initials: YT
authors.lastname: Zheng
mesh_terms: D019380:Anti-HIV Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D002460:Cell Line; D016655:HIV Core Protein p24 / Q000037:antagonists & inhibitors / Q000378:metabolism; D023581:HIV Fusion Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D015497:HIV-1 / Q000187:drug effects*; D006801:Humans; D017705:Lignans / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D008968:Molecular Conformation; D032141:Schisandra / Q000737:chemistry*; D014779:Virus Replication / Q000187:drug effects
chemical_list: D019380:Anti-HIV Agents; D016655:HIV Core Protein p24; D023581:HIV Fusion Inhibitors; D017705:Lignans; C556185:marphenol A; C556186:marphenol B