title: Pathogenicity of swine influenza viruses possessing an avian or swine-origin PB2 polymerase gene evaluated in mouse and pig models.
abstract: PB2 627K is a determinant of influenza host range and contributes to the pathogenicity of human-, avian-, and mouse-adapted influenza viruses in the mouse model. Here we used mouse and pig models to analyze the contribution of a swine-origin and avian-origin PB2 carrying either 627K or 627E in the background of the classical swine H1N1 (A/Swine/Iowa/15/30; 1930) virus. The results showed PB2 627K is crucial for virulence in the mouse model, independent of whether PB2 is derived from an avian or swine influenza virus (SIV). In the pig model, PB2 627E decreases pathogenicity of the classical 1930 SIV when it contains the swine-origin PB2, but not when it possesses the avian-origin PB2. Our study suggests the pathogenicity of SIVs with different PB2 genes and mutation of codon 627 in mice does not correlate with the pathogenicity of the same SIVs in the natural host, the pig.
authors.affiliation: Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506, USA.
authors.forename: Wenjun
authors.initials: W
authors.lastname: Ma
authors.forename: Kelly M
authors.initials: KM
authors.lastname: Lager
authors.forename: Xi
authors.initials: X
authors.lastname: Li
authors.forename: Bruce H
authors.initials: BH
authors.lastname: Janke
authors.forename: Derek A
authors.initials: DA
authors.lastname: Mosier
authors.forename: Laura E
authors.initials: LE
authors.lastname: Painter
authors.forename: Eva S
authors.initials: ES
authors.lastname: Ulery
authors.forename: Jingqun
authors.initials: J
authors.lastname: Ma
authors.forename: Porntippa
authors.initials: P
authors.lastname: Lekcharoensuk
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Webby
authors.forename: Jürgen A
authors.initials: JA
authors.lastname: Richt
mesh_terms: D000818:Animals; D002460:Cell Line; D004195:Disease Models, Animal; D004285:Dogs; D015967:Gene Expression Regulation, Viral / Q000502:physiology; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity*; D008168:Lung / Q000473:pathology / Q000821:virology; D051379:Mice; D008807:Mice, Inbred BALB C; D009154:Mutation; D009976:Orthomyxoviridae Infections / Q000821:virology*; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics* / Q000378:metabolism*; D016865:Reassortant Viruses; D013552:Swine; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism*; D014774:Virulence / Q000235:genetics; D014779:Virus Replication
chemical_list: C057027:PB2 protein, Influenzavirus A; D014764:Viral Proteins; D012324:RNA-Dependent RNA Polymerase