title: Interleukin-8 gene promoter polymorphism (rs4073) may contribute to chronic periodontitis.
abstract: BACKGROUND
  The proinflammatory chemokine interleukin (IL)-8 is important in the regulation of the inflammatory response. Analyses of the single nucleotide polymorphism (SNP) reference sequence (rs) 4073 showed that the A allele upregulated IL-8 levels after stimulation with lipopolysaccharides. We investigated the association of the SNP rs4073 with chronic periodontitis.
  
  
  METHODS
  Genotyping was performed by a standard polymerase chain reaction-restriction fragment length polymorphism assay in 289 genomic DNA samples of healthy control subjects and patients with chronic periodontitis; analyses were adjusted by multivariate logistic regression modeling. A real-time polymerase chain reaction performance was used to detect levels of the IL-8 mRNA.
  
  
  RESULTS
  The analysis pointed to a statistically significant association of chronic periodontitis with the heterozygous TA genotype (P = 0.001); the results showed an increase in the frequency of the A allele in the diseased group (36% in the control group versus 48% in the periodontitis group). The higher levels of the IL-8 mRNA were found in the periodontitis group, mainly in individuals who presented the TA genotype (P = 0.03).
  
  
  CONCLUSIONS
  The SNP rs4073 was associated with chronic periodontitis in non-smoker Brazilian subjects because the frequency of the A allele was higher in the disease group than in the control group, and the TA genotype was associated with increased levels of IL-8 mRNA transcripts.
authors.affiliation: Department of Morphology, Division of Histology, Laboratory of Molecular Biology, School of Dentistry at Piracicaba, State University of Campinas, Piracicaba, SP, Brazil. denise@andia.com.br
authors.forename: Denise C
authors.initials: DC
authors.lastname: Andia
authors.forename: Naila F P
authors.initials: NF
authors.lastname: de Oliveira
authors.forename: Ariadne M
authors.initials: AM
authors.lastname: Letra
authors.forename: Francisco H
authors.initials: FH
authors.lastname: Nociti
authors.forename: Sergio R P
authors.initials: SR
authors.lastname: Line
authors.forename: Ana P
authors.initials: AP
authors.lastname: de Souza
mesh_terms: D000328:Adult; D000483:Alleles; D001938:Brazil; D016022:Case-Control Studies; D055113:Chronic Periodontitis / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D006239:Haplotypes; D006801:Humans; D016209:Interleukin-8 / Q000096:biosynthesis / Q000235:genetics*; D008070:Lipopolysaccharides / Q000276:immunology; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018709:Statistics, Nonparametric; D015854:Up-Regulation
chemical_list: D016209:Interleukin-8; D008070:Lipopolysaccharides