title: Adipose-derived stromal cells: cytokine expression and immune cell contaminants.
abstract: The present method describes an immunoselection/depletion approach to isolate the native human adipose tissue-derived progenitor cells that are free from endothelial cells and immune cells by the use of magnetic nanobeads and microbeads coupled to antibodies. Moreover, methods to isolate and to analyse the distinct cell populations that constitute the microenvironment of the human adipose tissue progenitor cells, i.e. mature adipocytes, endothelial cells, and macrophages, are mentioned.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale (INSERM), Institut de Médecine Moléculaire de Rangueil, Université Toulouse III Paul-Sabatier, Toulouse, France.
authors.forename: Pauline
authors.initials: P
authors.lastname: Decaunes
authors.forename: David
authors.initials: D
authors.lastname: Estève
authors.forename: Alexia
authors.initials: A
authors.lastname: Zakaroff-Girard
authors.forename: Coralie
authors.initials: C
authors.lastname: Sengenès
authors.forename: Jean
authors.initials: J
authors.lastname: Galitzky
authors.forename: Anne
authors.initials: A
authors.lastname: Bouloumié
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000378:metabolism; D000273:Adipose Tissue / Q000166:cytology*; D018952:Antigens, CD34 / Q000378:metabolism; D002454:Cell Differentiation; D002469:Cell Separation / Q000379:methods*; D016207:Cytokines / Q000378:metabolism*; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D018950:Lipopolysaccharide Receptors / Q000378:metabolism; D008214:Lymphocytes / Q000166:cytology / Q000378:metabolism*; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism; D012313:RNA / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017154:Stromal Cells / Q000166:cytology / Q000378:metabolism
chemical_list: D018952:Antigens, CD34; D016207:Cytokines; D018950:Lipopolysaccharide Receptors; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D012313:RNA