title: Diffusion tensor driven contour closing for cell microinjection targeting.
abstract: This article introduces a novel approach to robust automatic detection of unstained living cells in bright-field (BF) microscope images with the goal of producing a target list for an automated microinjection system. The overall image analysis process is described and includes: preprocessing, ridge enhancement, image segmentation, shape analysis and injection point definition. The developed algorithm implements a new version of anisotropic contour completion (ACC) based on the partial differential equation (PDE) for heat diffusion which improves the cell segmentation process by elongating the edges only along their tangent direction. The developed ACC algorithm is equivalent to a dilation of the binary edge image with a continuous elliptic structural element that takes into account local orientation of the contours preventing extension towards normal direction. Experiments carried out on real images of 10 to 50 microm CHO-K1 adherent cells show a remarkable reliability in the algorithm along with up to 85% success for cell detection and injection point definition.
authors.affiliation: Italian Institute of Technology, Via Morego 30, 16163 Genova, Italy. gabriele.becattini@iit.it
authors.forename: Gabriele
authors.initials: G
authors.lastname: Becattini
authors.forename: Leonardo S
authors.initials: LS
authors.lastname: Mattos
authors.forename: Darwin G
authors.initials: DG
authors.lastname: Caldwell
mesh_terms: D000465:Algorithms; D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D004058:Diffusion; D008845:Microinjections