title: Biodegradation of 4-aminobenzenesulfonate by Ralstonia sp. PBA and Hydrogenophaga sp. PBC isolated from textile wastewater treatment plant.
abstract: A co-culture consisting of Hydrogenophaga sp. PBC and Ralstonia sp. PBA, isolated from textile wastewater treatment plant could tolerate up to 100 mM 4-aminobenzenesulfonate (4-ABS) and utilize it as sole carbon, nitrogen and sulfur source under aerobic condition. The biodegradation of 4-ABS resulted in the release of nitrogen and sulfur in the form of ammonium and sulfate respectively. Ninety-eight percent removal of chemical oxygen demand attributed to 20 mM of 4-ABS in cell-free supernatant could be achieved after 118 h. Effective biodegradation of 4-ABS occurred at pH ranging from 6 to 8. During batch culture with 4-ABS as sole carbon and nitrogen source, the ratio of strain PBA to PBC was dynamic and a critical concentration of strain PBA has to be reached in order to enable effective biodegradation of 4-ABS. Haldane inhibition model was used to fit the degradation rate at different initial concentrations and the parameters μ(max), K(s) and K(i) were determined to be 0.13 h⁻¹, 1.3 mM and 42 mM respectively. HPLC analyses revealed traced accumulation of 4-sulfocatechol and at least four unidentified metabolites during biodegradation. This is the first study to report on the characterization of 4-ABS-degrading bacterial consortium that was isolated from textile wastewater treatment plant.
authors.affiliation: Department of Industrial Biotechnology, Universiti Teknologi Malaysia, Johor, Malaysia. hxg2760@gmail.com
authors.forename: Han Ming
authors.initials: HM
authors.lastname: Gan
authors.forename: Shafinaz
authors.initials: S
authors.lastname: Shahir
authors.forename: Zaharah
authors.initials: Z
authors.lastname: Ibrahim
authors.forename: Adibah
authors.initials: A
authors.lastname: Yahya
mesh_terms: D001483:Base Sequence; D001673:Biodegradation, Environmental; D001711:Biotransformation; D042621:Comamonadaceae / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007220:Industrial Waste / Q000032:analysis*; D008969:Molecular Sequence Data; D010802:Phylogeny; D043367:Ralstonia / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D013425:Sulfanilic Acids / Q000378:metabolism*; D013783:Textile Industry; D014865:Waste Disposal, Fluid; D014871:Water Microbiology; D014874:Water Pollutants, Chemical / Q000378:metabolism*
chemical_list: D007220:Industrial Waste; D013425:Sulfanilic Acids; D014874:Water Pollutants, Chemical; C100016:4-sulfanilic acid