title: Aberrant methylation of CCAAT/enhancer binding protein zeta promoter in acute myeloid leukemia.
abstract: The aberrant changes of tumor suppressor genes (TSGs) are now recognized as an important mechanism contributing to the development of acute myeloid leukemia (AML). CCAAT/enhancer binding protein zeta (C/EBPζ), a candidate TSG, has been found to be involved in cancers including AML. We detected the methylation status of C/EBPζ promoter in 133 patients with AML using the methylation-specific polymerase chain reaction (MS-PCR) and examined C/EBPζ transcript in 32 patients using real-time quantitative PCR. The abnormal methylation of C/EBPζ gene promoter was found in 62 (46.6%) AML cases. No correlation was found between C/EBPζ promoter hypermethylation and the age, sex, WBC counts, platelet counts and FAB subtypes of AML patients (P>0.05). The trend that the frequency of C/EBPζ methylation increased as karyotype became more adverse was observed (R=0.167, P=0.075). There was a significant correlation between C/EBPζ expression and C/EBPζ methylation in AML patients (R=0.606, P=0.002). Our data suggest that the aberrant methylation of C/EBPζ promoter may be involved in AML.
authors.affiliation: Department of Hematology, Affiliated People's Hospital of Jiangsu University, 8 Dianli Rd, Zhenjiang, Jiangsu 212002, PR China.
authors.forename: Dong-Ming
authors.initials: DM
authors.lastname: Yao
authors.forename: Jun
authors.initials: J
authors.lastname: Qian
authors.forename: Jiang
authors.initials: J
authors.lastname: Lin
authors.forename: Ya-Li
authors.initials: YL
authors.lastname: Wang
authors.forename: Qin
authors.initials: Q
authors.lastname: Chen
authors.forename: Zhen
authors.initials: Z
authors.lastname: Qian
authors.forename: Yun
authors.initials: Y
authors.lastname: Li
authors.forename: Cui-Zhu
authors.initials: CZ
authors.lastname: Wang
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015153:Blotting, Western; D022762:CCAAT-Enhancer-Binding Proteins / Q000235:genetics*; D002648:Child; D002675:Child, Preschool; D019175:DNA Methylation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055815:Young Adult
chemical_list: D022762:CCAAT-Enhancer-Binding Proteins; C560326:CEBPZ protein, human; D012333:RNA, Messenger