title: Utilization of natural fucosylated oligosaccharides by three novel alpha-L-fucosidases from a probiotic Lactobacillus casei strain.
abstract: Three putative α-L-fucosidases encoded in the Lactobacillus casei BL23 genome were cloned and purified. The proteins displayed different abilities to hydrolyze natural fucosyloligosaccharides like 2'-fucosyllactose, H antigen disaccharide, H antigen type II trisaccharide, and 3'-, 4'-, and 6'-fucosyl-GlcNAc. This indicated a possible role in the utilization of oligosaccharides present in human milk and intestinal mucosa.
authors.affiliation: Laboratorio de Bacterias Lácticas y Probióticos, Instituto de Agroquímica y Tecnología de los Alimentos, Consejo Superior de Investigaciones Científicas, Paterna, Valencia, Spain. btcjrd@iata.csic.es
authors.forename: Jesús
authors.initials: J
authors.lastname: Rodríguez-Díaz
authors.forename: Vicente
authors.initials: V
authors.lastname: Monedero
authors.forename: María J
authors.initials: MJ
authors.lastname: Yebra
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D003001:Cloning, Molecular; D006801:Humans; D006868:Hydrolysis; D007780:Lacticaseibacillus casei / Q000201:enzymology*; D009844:Oligosaccharides / Q000378:metabolism*; D019936:Probiotics; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D005644:alpha-L-Fucosidase / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D009844:Oligosaccharides; D011994:Recombinant Proteins; D005644:alpha-L-Fucosidase