title: Impact of plasma and whole-blood anticoagulant counter ion choice on drug stability and matrix effects during bioanalysis.
abstract: BACKGROUND
  Anticoagulants are used to prevent coagulation in blood samples. The plasma pH may change with a different counter ion and anticoagulant; thus, it is essential to determine effects on drug stability and the matrix effect during the bioanalytical method development.
  
  
  RESULTS
  Cross-validation of multiple compounds between different counter ions was performed and no impact from the counter ion nature was demonstrated. Moreover, plasma stabilities and matrix effects for both fluconazole and granisetron were investigated thoroughly in numerous counter ions/anticoagulants (K(3)ethylenediaminetetraacetic acid [K(3)EDTA], K(2)EDTA, NaEDTA, NaHeparin and LiHeparin). Sirolimus, a large cyclic molecule, was also tested in different whole-blood EDTA counter ions. Results showed percentage deviation less than 8.5% and percentage cross-validation less than 8.4%.
  
  
  CONCLUSIONS
  None of the compounds tested had an impact on the matrix stabilities and matrix effect.
authors.affiliation: Algorithme Pharma Inc., 575 Armand-Frappier, Laval, Quebec, H7V 4B3, Canada. mbergeron@algopharm.com
authors.forename: Mélanie
authors.initials: M
authors.lastname: Bergeron
authors.forename: Annik
authors.initials: A
authors.lastname: Bergeron
authors.forename: Milton
authors.initials: M
authors.lastname: Furtado
authors.forename: Fabio
authors.initials: F
authors.lastname: Garofolo
mesh_terms: D000925:Anticoagulants / Q000097:blood* / Q000737:chemistry* / Q000494:pharmacology; D056148:Chromatography, Reverse-Phase; D004355:Drug Stability; D004492:Edetic Acid / Q000737:chemistry*; D015725:Fluconazole / Q000097:blood; D017829:Granisetron / Q000097:blood; D006493:Heparin / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007477:Ions; D057230:Limit of Detection; D004364:Pharmaceutical Preparations; D020123:Sirolimus / Q000097:blood; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D000925:Anticoagulants; D004492:Edetic Acid; D015725:Fluconazole; D017829:Granisetron; D006493:Heparin; D007477:Ions; D004364:Pharmaceutical Preparations; D020123:Sirolimus