title: Inhibition of transketolase by hexacyanoferrate(III).
abstract: The effect of hexacyanoferrate(III) on the catalytic activity of transketolase has been studied. This oxidant inactivates only one of two active sites of the enzyme, the one with a higher affinity to the coenzyme (thiamine diphosphate). The second active site does not lose its catalytic activity. These observations indicate that the active sites of holotransketolase, being indiscernible by data of X-ray analysis, exhibit functional nonequivalence.
authors.affiliation: Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, 119991, Russia.
authors.forename: V A
authors.initials: VA
authors.lastname: Yurshev
authors.forename: I A
authors.initials: IA
authors.lastname: Sevostyanova
authors.forename: O N
authors.initials: ON
authors.lastname: Solovjeva
authors.forename: G A
authors.initials: GA
authors.lastname: Kochetov
mesh_terms: D002384:Catalysis; D020134:Catalytic Domain; D005292:Ferricyanides / Q000737:chemistry / Q000494:pharmacology*; D007700:Kinetics; D013835:Thiamine Pyrophosphate / Q000737:chemistry / Q000378:metabolism; D014174:Transketolase / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000378:metabolism
chemical_list: D005292:Ferricyanides; C007931:hexacyanoferrate III; D014174:Transketolase; D013835:Thiamine Pyrophosphate