title: Nuclear magnetic resonance spectroscopy-based metabonomic investigation of biochemical effects in serum of γ-irradiated mice.
abstract: OBJECTIVE
  Radiation exposure induces change in many biological compounds. It is important to assess the physiological and biochemical response to an absorbed dose of ionising radiation due to intentional or accidental event and to predict medical consequences for medical management. In the present study, nuclear magnetic resonance (NMR) spectroscopy-based metabolic profiling was used in mice serum for identification of radiation-induced changes at metabolite level.
  
  
  METHODS
  Mice were irradiated with 3, 5 and 8 Gray of γ-radiation dose and serum samples collected at day 1, 3 and 5 post irradiation were analysed by proton nuclear magnetic resonance (¹H NMR) spectroscopy. ¹H NMR spectra of serum were analysed by pattern recognition using principal component analysis.
  
  
  RESULTS
  Irradiated mice serum showed distinct metabonomic phenotypes and revealed dose- and time-dependent clustering of irradiated groups. ¹H NMR spectral analysis exhibited increased lactate, amino acids, choline and lipid signals as well as decreased glucose signals. These findings indicate radiation-induced disturbed energy, lipid and protein metabolism.
  
  
  CONCLUSIONS
  The information obtained from this study reflects multiple physiological dysfunctions. The study promises the application of NMR-based metabonomics in the field of radiobiology, for development of metabolic-based markers for screening of risk populations and medical management in these cases.
authors.affiliation: NMR Research Centre, Timarpur, Delhi, India.
authors.forename: Ahmad Raza
authors.initials: AR
authors.lastname: Khan
authors.forename: Poonam
authors.initials: P
authors.lastname: Rana
authors.forename: M Memita
authors.initials: MM
authors.lastname: Devi
authors.forename: Shubhra
authors.initials: S
authors.lastname: Chaturvedi
authors.forename: Salim
authors.initials: S
authors.lastname: Javed
authors.forename: Rajendra P
authors.initials: RP
authors.lastname: Tripathi
authors.forename: Subash
authors.initials: S
authors.lastname: Khushu
mesh_terms: D000597:Amino Acids, Branched-Chain / Q000097:blood; D000818:Animals; D001769:Blood / Q000378:metabolism / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D004734:Energy Metabolism / Q000528:radiation effects; D005720:Gamma Rays / Q000009:adverse effects*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008297:Male; D055442:Metabolome / Q000528:radiation effects*; D055432:Metabolomics; D051379:Mice; D008805:Mice, Inbred A; D018384:Oxidative Stress / Q000528:radiation effects
chemical_list: D000597:Amino Acids, Branched-Chain