title: A robust system for production of minicircle DNA vectors.
abstract: Minicircle DNA vectors allow sustained transgene expression in quiescent cells and tissues. To improve minicircle production, we genetically modified Escherichia coli to construct a producer strain that stably expresses a set of inducible minicircle-assembly enzymes, ΦC31 integrase and I-SceI homing endonuclease. This bacterial strain produces purified minicircles in a time frame and quantity similar to those of routine plasmid DNA preparation, making it feasible to use minicircles in place of plasmids in mammalian transgene expression studies.
authors.affiliation: Department of Pediatrics and Genetics, Stanford University School of Medicine, Stanford, California, USA. markay@stanford.edu
authors.forename: Mark A
authors.initials: MA
authors.lastname: Kay
authors.forename: Cheng-Yi
authors.initials: CY
authors.lastname: He
authors.forename: Zhi-Ying
authors.initials: ZY
authors.lastname: Chen
mesh_terms: D001709:Biotechnology / Q000379:methods*; D003001:Cloning, Molecular / Q000379:methods*; D004270:DNA, Circular; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors; D010957:Plasmids
chemical_list: D004270:DNA, Circular