title: Closely related oxidized phospholipids differentially modulate the physicochemical properties of lipid particles.
abstract: Oxidation of glycerophospholipids results in the formation of large variety of oxidized phospholipid products that differs significantly in their chemical compositions and molecular structures. Biological activities of these oxidized products also differ considerably. Here we report the comparisons of the physicochemical properties of non-oxidized phospholipid particle containing two closely related tOx-PLs: 1-palmitoyl-2-(5-keto-6-octendioyl)-sn-glycero-3-phosphocholine (KOdiA-PC) and 1-palmitoyl-2-(9-keto-10-dodecendioyl)-sn-glycero-3-phosphocholine (KDdiA-PC). DMPC (1,2-dimyristoyl-sn-glycero-3-phosphocholine) was used as a model membrane non-oxidized phospholipid. Physicochemical properties of the lipid particles were characterized by using fluorescence spectroscopy, native polyacrylamide gel and agarose gel electrophoresis. Our result shows that the presence of closely related tOx-PLs, which differ only in the chemical composition of the oxidized fatty acyl chains at the sn-2 position, exerts considerably different effect on the physicochemical properties of non-oxidized phospholipid particles containing them.
authors.affiliation: Department of Biotechnology, National Institute of Pharmaceutical Education and Research, Sector 67, S.A.S. Nagar, Mohali, 160062 Punjab, India.
authors.forename: Subhabrata
authors.initials: S
authors.lastname: Kar
authors.forename: Vikas A
authors.initials: VA
authors.lastname: Tillu
authors.forename: Shiv Charan
authors.initials: SC
authors.lastname: Meena
authors.forename: Abhay H
authors.initials: AH
authors.lastname: Pande
mesh_terms: D008823:Micelles; D010084:Oxidation-Reduction; D010713:Phosphatidylcholines / Q000737:chemistry*; D010743:Phospholipids / Q000737:chemistry*; D013499:Surface Properties
chemical_list: C544326:1-palmitoyl-2-(4-ketododec-3-enedioyl)phosphatidylcholine; C544327:1-palmitoyl-2-(5-keto-6-octenedioyl)phosphatidylcholine; D008823:Micelles; D010713:Phosphatidylcholines; D010743:Phospholipids