title: Eimeria papillata: upregulation of specific miRNA-species in the mouse jejunum.
abstract: Increasing evidence indicates miRNAs as critical regulators of gene expression, but little information is available for miRNAs in intestinal diseases. Here, we investigated intestinal infections of male Balb/c mice with the coccidian parasite Eimeria papillata. On day 4 after oral infection, mice were shedding 3150 ± 430 oocysts per gram feces. This was associated with a low inflammatory response of the jejunum of mice evidenced by histology, non-response of IL-1β mRNA, even slight downregulation of IL-6 mRNA, only slight increases in iNOS mRNA, nitrate/nitrate, malondialdehyde, and a small decrease in glutathione, respectively. Only IFNγ mRNA was strongly induced. Using miRNA microarray technology, there were significantly upregulated the four miRNA species miR-1959, MCMV-miR-M23-1-5P, miR-203, and miR-21 out of 634 miRNAs, which was also confirmed by quantitative RT-PCR. Our data provide evidence that E. papillata parasites are able to induce specific miRNA species in their host target organ.
authors.affiliation: Department of Zoology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia. mohameddkhil@yahoo.com
authors.forename: Mohamed
authors.initials: M
authors.lastname: Dkhil
authors.forename: Abdel Azeem
authors.initials: AA
authors.lastname: Abdel-Baki
authors.forename: Denis
authors.initials: D
authors.lastname: Delić
authors.forename: Frank
authors.initials: F
authors.lastname: Wunderlich
authors.forename: Helmut
authors.initials: H
authors.lastname: Sies
authors.forename: Saleh
authors.initials: S
authors.lastname: Al-Quraishy
mesh_terms: D000818:Animals; D003048:Coccidiosis / Q000469:parasitology*; D004539:Eimeria / Q000235:genetics*; D007583:Jejunum / Q000378:metabolism / Q000469:parasitology* / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D035683:MicroRNAs / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction; D013047:Specific Pathogen-Free Organisms; D015854:Up-Regulation
chemical_list: D035683:MicroRNAs