title: [Mechanisms of resveratrol bovine serum albumin nanoparticle-induced cell death in human ovarian cancer SKOV3 cells].
abstract: OBJECTIVE
  To study the effect of resveratrol bovine serum albumin nanoparticles on SKOV3 cell line and its mechanisms.
  
  
  METHODS
  The morphological changes of the cells exposed to the nanoparticles were observed by apoptotic body/cell nucleus DNA staining under inverted microscope and fluorescence microscope, and the pathway of cell death was determined by phosphatidylserine translocation. Western blotting was performed to detect the activation of cyto.c, caspase-3 and caspase-9.
  
  
  RESULTS
  DNA ladder was detected with gel electrophoresis and the cell death was partially inhibited by the pan-caspase inhibitor Z-VAD-FMK. Gel electrophoresis displayed both DNA ladder and smear in RES-BSANP exposed groups, while DNA ladder disappeared in Z-VAD-FMK group and only the smear was left. Cyto.c in the cytoplasm was released at 2 h, while the expression of caspase-9 protein reached the peak level at 4 h and caspase-3 expression was obvious enhanced at 8 h. At 4 h, caspase-9 expression in the cells exposed to 100 µmol/L RES-BSANP was decreased significantly as compared to the cells treated with 50 µmol/L RES-BSANP (P>0.05).
  
  
  CONCLUSIONS
  RES-BSANP can induce the necrosis and apoptosis of SKOV3 cells via either caspase-dependent or caspase-independent pathways.
authors.affiliation: Third Hospital of Harbin Medical University, Harbin 150081, China. guoliyuan@163.com
authors.forename: Li-yuan
authors.initials: LY
authors.lastname: Guo
authors.forename: Yan
authors.initials: Y
authors.lastname: Peng
authors.forename: Yu-lian
authors.initials: YL
authors.lastname: Li
authors.forename: Jing-ping
authors.initials: JP
authors.lastname: Yao
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Guang-mei
authors.initials: GM
authors.lastname: Zhang
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
authors.forename: Li-hua
authors.initials: LH
authors.lastname: Sui
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D053453:Caspase 9 / Q000378:metabolism; D016923:Cell Death / Q000187:drug effects*; D045744:Cell Line, Tumor; D045304:Cytochromes c / Q000378:metabolism; D005260:Female; D006801:Humans; D053758:Nanoparticles; D010051:Ovarian Neoplasms / Q000473:pathology*; D000077185:Resveratrol; D013267:Stilbenes / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D013267:Stilbenes; D045304:Cytochromes c; C505407:CASP3 protein, human; C506433:CASP9 protein, human; D053148:Caspase 3; D053453:Caspase 9; D000077185:Resveratrol