title: Anti-apoptotic protein TCTP controls the stability of the tumor suppressor p53.
abstract: In this study, we identified p53 as a novel TCTP-interacting protein using TCTP as bait. Also, we determined the critical binding sites between TCTP and p53. To elucidate the functional consequence of the interaction, we developed the overexpression and inhibition system of TCTP and p53 expression. Overexpression of TCTP in lung carcinoma cells reversed p53 mediated apoptosis and inhibition of TCTP expression by small interfering RNA increased apoptosis of lung carcinoma cells. Moreover, it was observed that TCTP overexpression promotes degradation of p53. These results clearly indicate that the interaction between TCTP and p53 prevents apoptosis by destabilizing p53. Thus, TCTP acts as a negative regulator of apoptosis in lung cancer.
authors.affiliation: Research Institute, National Cancer Center, Ilsandong-gu, Goyang-si Gyeonggi-do, Republic of Korea. sbrho@ncc.re.kr
authors.forename: Seung Bae
authors.initials: SB
authors.lastname: Rho
authors.forename: Jeong Heon
authors.initials: JH
authors.lastname: Lee
authors.forename: Mi Sun
authors.initials: MS
authors.lastname: Park
authors.forename: Hyun-Jung
authors.initials: HJ
authors.lastname: Byun
authors.forename: Sokbom
authors.initials: S
authors.lastname: Kang
authors.forename: Sang-Soo
authors.initials: SS
authors.lastname: Seo
authors.forename: Joo-Young
authors.initials: JY
authors.lastname: Kim
authors.forename: Sang-Yoon
authors.initials: SY
authors.lastname: Park
mesh_terms: D000595:Amino Acid Sequence; D017209:Apoptosis; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000378:metabolism*; D001665:Binding Sites / Q000235:genetics; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D053938:DNA Fragmentation; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D047468:Immunoprecipitation; D008969:Molecular Sequence Data; D011485:Protein Binding; D025941:Protein Interaction Mapping; D055550:Protein Stability; D034622:RNA Interference; D014162:Transfection; D000090324:Tumor Protein, Translationally-Controlled 1; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
chemical_list: D051017:Apoptosis Regulatory Proteins; D014408:Biomarkers, Tumor; C000715647:TPT1 protein, human; D000090324:Tumor Protein, Translationally-Controlled 1; D016159:Tumor Suppressor Protein p53; D049452:Green Fluorescent Proteins