title: Clorgyline inhibits orexin-A-induced arousal in layer-type chicks.
abstract: We previously demonstrated that intracerebroventricular (ICV) injection of orexin-A induces arousal and increased metabolic turnover rates of norepinephrine, dopamine, and serotonin in layer (egg-type) chicks. Because monoamine oxidase-A (MAO-A) is a potent degrading enzyme of these monoamines, we hypothesized that orexin-A may mediate its arousal-inducing effects through MAO-A. Therefore, we simultaneously injected clorgyline, a specific inhibitor of MAO-A, with orexin-A and examined behavior of chicks. Behaviors associated with arousal were attenuated in the group of chicks that received clorgyline and orexin-A compared with those that received orexin-A alone. For the monoamine turnover rate, enhancement of the turnover rate of serotonin by orexin-A was attenuated by clorgyline. Therefore, we conclude that orexin-A-induced arousal is dependent upon monoamine neural activities stimulated by MAO-A in chicks.
authors.affiliation: Laboratory of Regulation in Metabolism and Behavior, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Fukuoka, Japan.
authors.forename: Sachiko
authors.initials: S
authors.lastname: Katayama
authors.forename: Kazutaka
authors.initials: K
authors.lastname: Shigemi
authors.forename: Mark A
authors.initials: MA
authors.lastname: Cline
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Furuse
mesh_terms: D000818:Animals; D001143:Arousal / Q000187:drug effects*; D015306:Biogenic Monoamines / Q000378:metabolism; D002645:Chickens; D003010:Clorgyline / Q000494:pharmacology*; D007030:Hypothalamo-Hypophyseal System / Q000187:drug effects; D047908:Intracellular Signaling Peptides and Proteins / Q000494:pharmacology*; D008297:Male; D008996:Monoamine Oxidase Inhibitors / Q000494:pharmacology; D009479:Neuropeptides / Q000494:pharmacology*; D018377:Neurotransmitter Agents / Q000494:pharmacology; D000068797:Orexins
chemical_list: D015306:Biogenic Monoamines; D047908:Intracellular Signaling Peptides and Proteins; D008996:Monoamine Oxidase Inhibitors; D009479:Neuropeptides; D018377:Neurotransmitter Agents; D000068797:Orexins; D003010:Clorgyline