title: Inorganic polyphosphate in the yeast Saccharomyces cerevisiae with a mutation disturbing the function of vacuolar ATPase.
abstract: A mutation in the vma2 gene disturbing V-ATPase function in the yeast Saccharomyces cerevisiae results in a five- and threefold decrease in inorganic polyphosphate content in the stationary and active phases of growth on glucose, respectively. The average polyphosphate chain length in the mutant cells is decreased. The mutation does not prevent polyphosphate utilization during cultivation in a phosphate-deficient medium and recovery of its level on reinoculation in complete medium after phosphate deficiency. The content of short chain acid-soluble polyphosphates is recovered first. It is supposed that these polyphosphates are less dependent on the electrochemical gradient on the vacuolar membrane.
authors.affiliation: Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia.
authors.forename: A A
authors.initials: AA
authors.lastname: Tomaschevsky
authors.forename: L P
authors.initials: LP
authors.lastname: Ryasanova
authors.forename: T V
authors.initials: TV
authors.lastname: Kulakovskaya
authors.forename: I S
authors.initials: IS
authors.lastname: Kulaev
mesh_terms: D009154:Mutation; D011122:Polyphosphates / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism; D025262:Vacuolar Proton-Translocating ATPases / Q000235:genetics* / Q000378:metabolism
chemical_list: D011122:Polyphosphates; D029701:Saccharomyces cerevisiae Proteins; C556537:VMA2 protein, S cerevisiae; D025262:Vacuolar Proton-Translocating ATPases