title: Influence of drying methods over in vitro antitumoral effects of exopolysaccharides produced by Agaricus blazei LPB 03 on submerged fermentation.
abstract: Agaricus blazei is a mushroom that belongs to the Brazilian biodiversity and is considered as an important producer of bioactive compounds beneficial to human health. Studies have demonstrated that these compounds present immuno-modulatory, antioxidant and antitumor properties. In order to compare the most used method for fungal polysaccharide drying, lyophilization with other industrial-scale methods, the aim of this work was to submit A. blazei LPB 03 polysaccharide extracts to vaucum, spray and freeze drying, and evaluate the maintenance of its antitumoral effects in vitro. Exopolysaccharides produced by A. blazei LPB 03 on submerged fermentation were extracted with ethanol and submitted to drying processes. The efficiency represents the water content that was removed during the drying process. The resultant dried products showed water content around 3% and water activity less than 0.380, preventing therefore the growth of microorganisms and reactions of chemical degradation. Exopolysaccharide extracts dried by vacuum and spray dryer did not showed any significant cytotoxic effect on cell viability of Wistar mice macrophages. Content of total sugars and protein decrease after drying, nevertheless, 20 mg/ml of exopolysaccharides dried by spray dryer reached 33% of inhibition rate over Ehrlich tumor cells in vitro.
authors.affiliation: Bioprocess Engineering and Biotechnology Division, Federal University of Paraná, UFPR, Post Office Box 19011, Curitiba, PR 81531-990, Brazil.
authors.forename: M B A
authors.initials: MB
authors.lastname: Fernandes
authors.forename: S
authors.initials: S
authors.lastname: Habu
authors.forename: M A
authors.initials: MA
authors.lastname: de Lima
authors.forename: V
authors.initials: V
authors.lastname: Thomaz-Soccol
authors.forename: C R
authors.initials: CR
authors.lastname: Soccol
mesh_terms: D000364:Agaricus / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism; D000818:Animals; D000970:Antineoplastic Agents / Q000302:isolation & purification* / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D005260:Female; D005285:Fermentation; D005612:Freeze Drying / Q000379:methods*; D005656:Fungal Proteins / Q000032:analysis; D008264:Macrophages; D051379:Mice; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D011134:Polysaccharides / Q000096:biosynthesis / Q000302:isolation & purification* / Q000494:pharmacology*; D014867:Water / Q000737:chemistry / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D005656:Fungal Proteins; D010936:Plant Extracts; D011134:Polysaccharides; D014867:Water