title: In vitro study of the antioxidative properties of the glucose derivatives against oxidation of plasma components.
abstract: Oxidative stress has been implicated in the pathogenesis of variety of diseases. Since the endogenous antioxidant defense may be not adequate to counteract the enhanced generation of oxidants, a growing interest in research for exogenous nutrients has been observed. The present study was designed to assess in vitro the antioxidative properties of the glucose derivatives: calcium D-glucarate, D-gluconic acid lactone, and sodium D-gluconate (0.5-3 mM) in the protection of plasma proteins and lipids, against the damage caused by 0.1 mM peroxynitrite (ONOO⁻). Exposure of plasma to ONOO⁻ resulted in carbonyl groups increase, 3-nitrotyrosine (3-NT) formation, reduction in thiol groups, and enhanced lipid peroxidation. D-gluconic acid lactone and sodium D-gluconate effectively decreased 3-NT formation; the antinitrative action of calcium D-glucarate was less effective. In plasma samples incubated with ONOO⁻ and tested compounds, the level of carbonyl groups was decreased in comparison to plasma samples treated only with ONOO⁻. The level of protein -SH groups and glutathione was significantly higher in the presence of glucose derivatives than in plasma samples treated with ONOO⁻ only. All the tested compounds had the inhibitory effect on the peroxynitrite-induced plasma lipids peroxidation. The results obtained from our work indicate that calcium D-glucarate, D-gluconic acid lactone, and sodium D-gluconate may partly protect plasma proteins and lipids against peroxynitrite-induced damages.
authors.affiliation: Department of General Biochemistry, Institute of Biochemistry, University of Lodz, Banacha 12/16, 90-237 Lodz, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Kolodziejczyk
authors.forename: Joanna
authors.initials: J
authors.lastname: Saluk-Juszczak
authors.forename: Barbara
authors.initials: B
authors.lastname: Wachowicz
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism; D001798:Blood Proteins / Q000378:metabolism*; D005937:Glucaric Acid / Q000737:chemistry / Q000494:pharmacology*; D005942:Gluconates / Q000737:chemistry / Q000494:pharmacology*; D005947:Glucose / Q000031:analogs & derivatives / Q000494:pharmacology; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D030421:Peroxynitrous Acid / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D000975:Antioxidants; D001798:Blood Proteins; D005942:Gluconates; D017392:Thiobarbituric Acid Reactive Substances; D030421:Peroxynitrous Acid; D005947:Glucose; D005937:Glucaric Acid; C030691:gluconic acid