title: Homonuclear mixing sequences for perdeuterated proteins.
abstract: We tested the performance of several (13)C homonuclear mixing sequences on perdeuterated microcrystalline ubiquitin. All sequences were applied without (1)H decoupling and at relatively low MAS frequencies. We found that RFDR gave the highest overall transfer efficiency and that DREAM performs surprisingly well under these conditions being twice as efficient in the aliphatic region of the spectrum than the other mixing sequences tested.
authors.affiliation: Department of Chemistry MC3113, Columbia University, 3000 Broadway, New York, NY 10027, USA.
authors.forename: Kuo-Ying
authors.initials: KY
authors.lastname: Huang
authors.forename: Ansgar B
authors.initials: AB
authors.lastname: Siemer
authors.forename: Ann E
authors.initials: AE
authors.lastname: McDermott
mesh_terms: D000595:Amino Acid Sequence; D003903:Deuterium / Q000032:analysis* / Q000737:chemistry*; D041961:Deuterium Exchange Measurement / Q000379:methods*; D007553:Isotope Labeling / Q000379:methods; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D025801:Ubiquitin / Q000032:analysis* / Q000737:chemistry*
chemical_list: D025801:Ubiquitin; D003903:Deuterium