title: Characteristics of high-molecular-weight hyaluronic acid as a brain-derived neurotrophic factor scaffold in periodontal tissue regeneration.
abstract: Brain-derived neurotrophic factor (BDNF), for which bovine collagen-derived atelocollagen is used as a scaffold, enhances periodontal tissue regeneration. However, a scaffold that does not contain unknown ingredients is preferable. Since the synthesized high-molecular-weight (HMW)-hyaluronic acid (HA) is safe and inexpensive, we evaluated the efficacy of HMW-HA as a BDNF scaffold. CD44, a major receptor of HA, was expressed in cultures of human periodontal ligament cells, and HMW-HA promoted the adhesion and proliferation of human periodontal ligament cells, although it did not influence the mRNA expression of bone (cementum)-related proteins. The in vitro release kinetics of BDNF from HMW-HA showed that BDNF release was sustained for 14 days. Subsequently, we examined the effect of BDNF/HMW-HA complex on periodontal tissue regeneration in dogs. A greater volume of newly formed alveolar bone and a longer newly formed cementum were observed in the BDNF/HMW-HA group than in the HMW-HA group, suggesting that HMW-HA assists the regenerative capacity of BDNF, although HMW-HA itself does not enhance periodontal tissue regeneration. Neither the poly (lactic-co-glycolic acid) group nor the BDNF/poly (lactic-co-glycolic acid) group enhanced periodontal tissue regeneration. In conclusion, HMW-HA is an adequate scaffold for the clinical application of BDNF.
authors.affiliation: Division of Frontier Medical Science, Department of Periodontal Medicine, Hiroshima University Graduate School of Biomedical Sciences, Minami-ku, Hiroshima, Japan. takepon@hiroshima-u.ac.jp
authors.forename: Katsuhiro
authors.initials: K
authors.lastname: Takeda
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Sakai
authors.forename: Hideki
authors.initials: H
authors.lastname: Shiba
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Nagahara
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Fujita
authors.forename: Mikihito
authors.initials: M
authors.lastname: Kajiya
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Iwata
authors.forename: Shinji
authors.initials: S
authors.lastname: Matsuda
authors.forename: Kazuko
authors.initials: K
authors.lastname: Kawahara
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kawaguchi
authors.forename: Hidemi
authors.initials: H
authors.lastname: Kurihara
mesh_terms: D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000737:chemistry* / Q000494:pharmacology; D002417:Cattle; D002448:Cell Adhesion / Q000235:genetics / Q000502:physiology; D002478:Cells, Cultured; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry* / Q000494:pharmacology; D007150:Immunohistochemistry; D008856:Microscopy, Fluorescence; D008970:Molecular Weight; D010519:Periodontium / Q000166:cytology*; D012038:Regeneration / Q000187:drug effects; D020133:Reverse Transcriptase Polymerase Chain Reaction; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D006820:Hyaluronic Acid