title: [Preparation of immunomagnetic nanoparticles for tumor targeting therapy with herceptin].
abstract: OBJECTIVE
  To prepare immunomagnetic nanoparticles (IMNs) for HER2/neu-targeted radioimmunotherapy with herceptin, a humanized anti-p185-HER-2/neu monoclonal antibody targeting the extracellular domain of HER-2/neu receptor.
  
  
  METHODS
  The magnetic nanoparticles were synthesized by partial reductive precipitation method and the surface of the particles was chemically modified using silane coupling agent. Herceptin and histidine were covalently linked to the amine group upon the silica-coated magnetic nanoparticles modified by N-[3-(trimethoxysilyl) propyl]-ethylenediamine using glutaraldehyde method to prepare the IMNs. The nanoparticles were evaluated by diffraction (XRD), scanning electron microscope (SEM) and X- ray energy spectrometry (EDS), and the immunoreactivity of IMN was determined.
  
  
  RESULTS
  The average diameter of the decanoic acid-coated Fe(3)O(4) nanoparticle was about 20 nm with a magnetic saturation of 65 emu/g. The surface amino group was 0.5 µmol/mg after modification with the amid functional group, and the mean size of Herceptin-loaded IMNs was about 60 nm. The IMN retained good immunoreactivity of Herceptin.
  
  
  CONCLUSIONS
  The IMNs exhibit good properties for potential application in tumor targeting therapy using Herceptin against HER-2/nue proto-oncogene.
authors.affiliation: Department of Nuclear Medicine, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. ligp62@yahoo.com.cn
authors.forename: Gui-ping
authors.initials: GP
authors.lastname: Li
authors.forename: Kai
authors.initials: K
authors.lastname: Huang
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.forename: Feng
authors.initials: F
authors.lastname: Liu
authors.forename: Xu-jian
authors.initials: XJ
authors.lastname: Chen
mesh_terms: D061067:Antibodies, Monoclonal, Humanized / Q000737:chemistry*; D004337:Drug Carriers / Q000138:chemical synthesis*; D008280:Magnetics; D053758:Nanoparticles / Q000737:chemistry*; D000068878:Trastuzumab
chemical_list: D061067:Antibodies, Monoclonal, Humanized; D004337:Drug Carriers; D000068878:Trastuzumab