title: Steroidal saponins from Yucca gloriosa L. rhizomes: LC-MS profiling, isolation and quantitative determination.
abstract: The occurrence of steroidal saponins in the rhizomes of Yucca gloriosa has been detected by LC-MS. On the basis of the LC-MS analysis, five steroidal glycosides, including three spirostane, one furostane and one cholestane glycosides, along with seven known compounds have been isolated and characterized by ESI-MS and by the extensive use of 1D- and 2D-NMR experiments. Quantitative analysis of the steroidal glycosides in Y. gloriosa rhizomes was performed by an LC-MS method validated according to European Medicines Agency (EMEA) guidelines. The dried BuOH extract obtained from rhizomes contains more than 25% w/w of glycosides, thus Y. gloriosa rhizomes can be considered a rich source of steroidal glycosides.
authors.affiliation: Iovel Kutateladze Institute of Pharmacochemistry, Tbilisi, Georgia.
authors.forename: Alexandre
authors.initials: A
authors.lastname: Skhirtladze
authors.forename: Angela
authors.initials: A
authors.lastname: Perrone
authors.forename: Paola
authors.initials: P
authors.lastname: Montoro
authors.forename: Mariam
authors.initials: M
authors.lastname: Benidze
authors.forename: Ether
authors.initials: E
authors.lastname: Kemertelidze
authors.forename: Cosimo
authors.initials: C
authors.lastname: Pizza
authors.forename: Sonia
authors.initials: S
authors.lastname: Piacente
mesh_terms: D004578:Electron Spin Resonance Spectroscopy; D015394:Molecular Structure; D027343:Rhizome / Q000737:chemistry; D012503:Saponins / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification*; D013256:Steroids / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification*; D027623:Yucca / Q000737:chemistry*
chemical_list: D012503:Saponins; D013256:Steroids