title: Effect of sustained release of bone morphogenetic protein on osteogenic expression of mesenchymal stem cells.
abstract: The objective of this work was to investigate the effect of sustained release of rhBMP-2, grafted to biodegradable nanoparticles (NPs) based on osteogenic differentiation of bone mesenchymal stem (BMS) cells. The release kinetics of rhBM-2 was linear with incubation time in the first two weeks. rhBMP-2 grafted NPs were as effective in inducing mineralization as the rhBMP-2 directly added to the culture media. rhBMP-2 grafted NPs had much higher expression of osteogenic markers osteopontin (OP) and osteocalcin (OC), compared to the protein directly added to culture media.
authors.affiliation: Univ. of South Carolina, USA.
authors.forename: Esmaiel
authors.initials: E
authors.lastname: Jabbari
mesh_terms: D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000008:administration & dosage*; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D003692:Delayed-Action Preparations / Q000008:administration & dosage*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects*; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects*; D010012:Osteogenesis / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D003692:Delayed-Action Preparations