title: The miR-30 family microRNAs confer epithelial phenotype to human pancreatic cells.
abstract: Epithelial-to-mesenchymal transition is a phenomenon necessary for embryonic development and also seen during certain pathological conditions.  We show here for the first time that reduction in miR-30 family microRNAs, is responsible for mesenchymal transition of primary cultures of human pancreatic epithelial cells.  We found that miR-30 family microRNAs target mesenchymal gene transcripts and maintain them in a translationally inactive state.  Forced depletion using miR-30 family specific anti-miRs leads to mesenchymal transition while ectopic overexpression maintains the epithelial phenotype.  We also show that miR-30 family microRNAs increase in abundance during differentiation of pancreatic islet-derived mesenchymal cells into hormone-producing islet-like cell aggregates.  Our studies in human adult diseased pancreas also demonstrate that miR-30 family microRNAs are expressed at lower abundance in fibrotic lesions during pancreatitis.  Together, our data confirm that miR-30 family microRNAs form a part of the regulatory signaling events involved in cellular response of pancreatic epithelial cells during mesenchymal transition.
authors.affiliation: Stem Cells and Diabetes Section, National Center for Cell Science, Ganeshkhind Road, Pune, India.
authors.forename: Mugdha V
authors.initials: MV
authors.lastname: Joglekar
authors.forename: Deepak
authors.initials: D
authors.lastname: Patil
authors.forename: Vinay M
authors.initials: VM
authors.lastname: Joglekar
authors.forename: G V
authors.initials: GV
authors.lastname: Rao
authors.forename: D Nageshwar
authors.initials: DN
authors.lastname: Reddy
authors.forename: Sasikala
authors.initials: S
authors.lastname: Mitnala
authors.forename: Yogesh
authors.initials: Y
authors.lastname: Shouche
authors.forename: Anandwardhan A
authors.initials: AA
authors.lastname: Hardikar
mesh_terms: D000328:Adult; D001483:Base Sequence; D054337:Cell Dedifferentiation / Q000235:genetics; D002454:Cell Differentiation / Q000235:genetics; D002478:Cells, Cultured; D016000:Cluster Analysis; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism* / Q000502:physiology*; D005333:Fetus / Q000166:cytology / Q000378:metabolism; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000378:metabolism / Q000502:physiology; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D046228:Microarray Analysis; D008954:Models, Biological; D005810:Multigene Family / Q000502:physiology; D010179:Pancreas / Q000166:cytology* / Q000378:metabolism*; D010641:Phenotype
chemical_list: C546086:MIRN30b microRNA, human; D035683:MicroRNAs