title: Effect of laser and LED on enzymatic production of ceramide.
abstract: An enzyme (Phospholipase C Type I from Clostridium perfringens) was exposed to 0-810Jcm(-2) of energy using laser light at wavelengths 808, 532, 1064 and 1342nm and two LED light sources at wavelengths 810 and 640nm. Enzyme responses were evaluated by measuring ceramide concentration using high performance thin-layer chromatography (HPTLC) at 0.5, 1, 2, 3, 4, 6, 17, 24h after irradiation. The duration of effect was evaluated from the experimental data. The results show that enzyme activity can be increased by using both laser and LED sources whose wavelength is located within a certain range. The effect depends on the energy and wavelength of the light. The increase in enzyme activity continued for about 4h after irradiation. This study shows that the duration of irradiation should be included as one of the main laser parameters when reporting on the effects of laser irradiation on enzymes. We also find that laser sources and LED sources have the same effect on enzyme activity if the wavelength and absorbed energy are equal.
authors.affiliation: Shanxi Province Tumor Hospital, Taiyuan, Shanxi Province, China. hongyushen2008@yahoo.com.cn
authors.forename: Hongyu
authors.initials: H
authors.lastname: Zhang
authors.forename: Long
authors.initials: L
authors.lastname: Zhang
authors.forename: Peter
authors.initials: P
authors.lastname: Tidemand-Lichtenberg
authors.forename: Preben
authors.initials: P
authors.lastname: Buchhave
authors.forename: Xuebing
authors.initials: X
authors.lastname: Xu
authors.forename: Yingxin
authors.initials: Y
authors.lastname: Li
mesh_terms: D002518:Ceramides / Q000378:metabolism*; D002855:Chromatography, Thin Layer; D003016:Clostridium perfringens / Q000201:enzymology; D007834:Lasers; D010738:Type C Phospholipases / Q000378:metabolism
chemical_list: D002518:Ceramides; D010738:Type C Phospholipases