title: Emergence of clonal groups O1:HNM-D-ST59, O15:H1-D-ST393, O20:H34/HNM-D-ST354, O25b:H4-B2-ST131 and ONT:H21,42-B1-ST101 among CTX-M-14-producing Escherichia coli clinical isolates in Galicia, northwest Spain.
abstract: CTX-M enzymes, mainly CTX-M-14 and CTX-M-15, have emerged as the most prevalent extended-spectrum β-lactamase (ESBL) type produced by Escherichia coli in Spain, with successful dissemination of clonal group O25b:H4-B2-ST131 producing CTX-M-15 within the hospital and community settings. However, until now CTX-M-14-producing E. coli in Spain had been shown to belong to a wide variety of serotypes with no predominance of a certain clonal group. In the present study, 654 E. coli strains positive for ESBL production obtained between 2005 and 2008 from inpatients and outpatients of four hospitals in Galicia, northwest Spain, were analysed. The strains were characterised with regard to ESBL enzymes, serotype, virulence genes, phylogenetic group, multilocus sequence type, and pulsed-field gel electrophoresis of XbaI-digested DNA. As a result, the emergence of certain clonal groups of extraintestinal pathogenic E. coli producing CTX-M-14 has been detected in this geographic area, including O1:HNM-D-ST59, O15:H1-D-ST393/ST1394, O20:H34/HNM-D-ST354, O25b:H4-B2-ST131 and ONT:H21,42-B1-ST101. These five clonal groups showed a high virulence potential as they harboured more than eight virulence factors, which could explain their successful dissemination.
authors.affiliation: Laboratorio de Referencia de E. coli (LREC), Departamento de Microbioloxía e Parasitoloxía, Facultade de Veterinaria, Universidade de Santiago de Compostela, Lugo, Spain.
authors.forename: Azucena
authors.initials: A
authors.lastname: Mora
authors.forename: Miguel
authors.initials: M
authors.lastname: Blanco
authors.forename: Cecilia
authors.initials: C
authors.lastname: López
authors.forename: Rosalia
authors.initials: R
authors.lastname: Mamani
authors.forename: Jesús E
authors.initials: JE
authors.lastname: Blanco
authors.forename: María Pilar
authors.initials: MP
authors.lastname: Alonso
authors.forename: Fernando
authors.initials: F
authors.lastname: García-Garrote
authors.forename: Ghizlane
authors.initials: G
authors.lastname: Dahbi
authors.forename: Alexandra
authors.initials: A
authors.lastname: Herrera
authors.forename: Ana
authors.initials: A
authors.lastname: Fernández
authors.forename: Begoña
authors.initials: B
authors.lastname: Fernández
authors.forename: Andrés
authors.initials: A
authors.lastname: Agulla
authors.forename: Germán
authors.initials: G
authors.lastname: Bou
authors.forename: Jorge
authors.initials: J
authors.lastname: Blanco
mesh_terms: D015373:Bacterial Typing Techniques; D016000:Cluster Analysis; D003428:Cross Infection / Q000453:epidemiology / Q000382:microbiology; D016521:Electrophoresis, Gel, Pulsed-Field; D004926:Escherichia coli / Q000145:classification* / Q000201:enzymology* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000453:epidemiology* / Q000382:microbiology*; D005838:Genotype; D006761:Hospitals; D006801:Humans; D017720:Molecular Epidemiology; D058885:Multilocus Sequence Typing; D010802:Phylogeny; D012703:Serotyping; D013030:Spain / Q000453:epidemiology; D037521:Virulence Factors / Q000235:genetics; D001618:beta-Lactamases / Q000096:biosynthesis*
chemical_list: D037521:Virulence Factors; C456080:beta-lactamase CTX-M-14; D001618:beta-Lactamases