title: Determination of naproxen using DBS: evaluation & pharmacokinetic comparison of human plasma versus human blood DBS.
abstract: BACKGROUND
  Dried blood spots (DBS) sampling is a well-known technology for qualitative determination such as DNA analysis and screening of newborn metabolic disorders. The scientific community has recently expressed interest in applying the DBS technique for quantitative determination of drugs in biological fluid.
  
  
  RESULTS
  Two new bioanalytical assays were developed and validated for the determination of naproxen in human plasma and in DBS samples using liquid chromatography coupled with tandem MS. Furthermore, plasma and DBS clinical samples were collected from four subjects enrolled as part of a bioequivalence study. Concentration data for plasma and DBS samples were determined and pharmacokinetic (PK) profiles in plasma and in DBS samples were compared.
  
  
  CONCLUSIONS
  A strong correlation between PK data obtained by the DBS and conventional plasma method was observed, which makes DBS a valuable technique for further naproxen bioavailability and PK investigations and studies.
authors.affiliation: Algorithme Pharma Inc., 575 Armand-Frappier, Laval, Quebec, H7V 4B3, Canada.
authors.forename: Nikolay
authors.initials: N
authors.lastname: Youhnovski
authors.forename: Josée
authors.initials: J
authors.lastname: Michon
authors.forename: Sylvain
authors.initials: S
authors.lastname: Latour
authors.forename: Jean-Nicholas
authors.initials: JN
authors.lastname: Mess
authors.forename: Annik
authors.initials: A
authors.lastname: Bergeron
authors.forename: Milton
authors.initials: M
authors.lastname: Furtado
authors.forename: Marianne
authors.initials: M
authors.lastname: Rufiange
authors.forename: Pascal
authors.initials: P
authors.lastname: Guibord
authors.forename: Marc
authors.initials: M
authors.lastname: Lefebvre
authors.forename: Robert B
authors.initials: RB
authors.lastname: Macarthur
authors.forename: Fabio
authors.initials: F
authors.lastname: Garofolo
mesh_terms: D001774:Blood Chemical Analysis / Q000379:methods* / Q000592:standards; D001798:Blood Proteins / Q000737:chemistry; D001800:Blood Specimen Collection / Q000295:instrumentation / Q000379:methods*; D011232:Chemical Precipitation; D002853:Chromatography, Liquid; D018849:Controlled Clinical Trials as Topic; D003890:Desiccation; D004355:Drug Stability; D005260:Female; D006801:Humans; D016014:Linear Models; D008297:Male; D009288:Naproxen / Q000008:administration & dosage / Q000097:blood* / Q000378:metabolism / Q000493:pharmacokinetics*; D012015:Reference Standards; D012044:Regression Analysis; D015203:Reproducibility of Results; D012995:Solubility; D053719:Tandem Mass Spectrometry
chemical_list: D001798:Blood Proteins; D009288:Naproxen