title: Transgenesis in mouse embryonic stem cells.
abstract: Traditionally, transgenic mice are generated by pronuclear injection of exogenous DNA. This technique has several limitations, including limited control over transgene expression, transgene cytotoxicity, -promiscuity and silencing, and founder mouse sterility. Here we describe two protocols to generate transgenic mice from ES cell clones carrying stably integrated exogenous DNA with inducible transgene expression.
authors.affiliation: Department of Pediatrics and Adolescent Medicine, Mayo Clinic College of Medicine, Rochester, MN, USA. vanree.janine@mayo.edu
authors.forename: Janine
authors.initials: J
authors.lastname: van Ree
authors.forename: Wei
authors.initials: W
authors.lastname: Zhou
authors.forename: Ming
authors.initials: M
authors.lastname: Li
authors.forename: Jan M
authors.initials: JM
authors.lastname: van Deursen
mesh_terms: D000818:Animals; D004318:Doxycycline / Q000494:pharmacology; D053595:Embryonic Stem Cells / Q000187:drug effects / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects / Q000235:genetics; D018014:Gene Transfer Techniques; D019426:Integrases / Q000235:genetics; D051379:Mice
chemical_list: C045073:Cre recombinase; D019426:Integrases; D004318:Doxycycline