title: Mid1p-dependent regulation of the M-G1 transcription wave in fission yeast.
abstract: The control of gene expression at certain times during the mitotic cell division cycle is a common feature in eukaryotes. In fission yeast, at least five waves of gene expression have been described, with one transcribed at the M-G1 interval under the control of the PBF transcription factor complex. PBF consists of at least three transcription factors, two forkhead-like proteins Sep1p and Fkh2p, and a MADS box-like protein Mbx1p, and binds to PCB motifs found in the gene promoters. Mbx1p is under the direct control of the polo-like kinase Plo1p and the Cdc14p-like phosphatase Clp1p (Flp1p). Here, we show that M-G1 gene expression in fission yeast is also regulated by the anillin-like protein, Mid1p (Dmf1p). Mid1p binds in vivo to both Fkh2p and Sep1p, and to the promoter regions of M-G1 transcribed genes. Mid1p promoter binding is dependent on Fkh2p, Plo1p and Clp1p. The absence of mid1(+) in cells results in partial loss of M-G1 specific gene expression, suggesting that it has a negative role in controlling gene expression. This phenotype is exacerbated by also removing clp1(+), suggesting that Mid1p and Clp1p have overlapping functions in controlling transcription. As mid1(+) is itself expressed at M-G1, these observations offer a new mechanism whereby Mid1p contributes to controlling cell cycle-specific gene expression as part of a feedback loop.
authors.affiliation: Division of Molecular and Cellular Biology, Faculty of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, UK.
authors.forename: Monica
authors.initials: M
authors.lastname: Agarwal
authors.forename: Kyriaki
authors.initials: K
authors.lastname: Papadopoulou
authors.forename: Adeline
authors.initials: A
authors.lastname: Mayeux
authors.forename: Vasanthi
authors.initials: V
authors.lastname: Vajrala
authors.forename: Daniela M
authors.initials: DM
authors.lastname: Quintana
authors.forename: Anne
authors.initials: A
authors.lastname: Paoletti
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: McInerny
mesh_terms: D016193:G1 Phase / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal / Q000235:genetics; D008938:Mitosis / Q000235:genetics*; D008957:Models, Genetic; D009154:Mutation / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding; D012568:Schizosaccharomyces / Q000166:cytology* / Q000235:genetics*; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: C489694:Mid1 protein, S pombe; D029702:Schizosaccharomyces pombe Proteins