title: Administration of granulocyte colony-stimulating factor ameliorates radiation-induced hepatic fibrosis in mice.
abstract: On the basis of the recent report that granulocyte colony-stimulating factor (G-CSF) treatment significantly improves survival and liver histology among chemically injured mice, we investigated whether G-CSF administration could contribute to faster recovery and promote tissue repair after local liver irradiation. Bone marrow chimeric female C57BL/6 mice were treated with G-CSF at days 7, 14, and 21 after local liver irradiation. We assessed the fibrosis index and the origin of proliferating cells reconstituting the liver at 2 or 5 weeks after radiation challenge. At day 35 after local irradiation, we observed G-CSF treatment to significantly reduce radiation-induced liver damage and collagen deposition. In addition, hepatic hydroxyproline levels and serum fibrosis markers in mice receiving G-CSF administration after radiation challenge were significantly lower compared with those of control mice. More importantly, histological examination suggested that recovery from hepatic damage was much better among the G-CSF-treated mice. Immunofluorescence and fluorescence in situ hybridization analyses revealed that donor cells engrafted into the host liver displayed epithelium-like morphology and expressed albumin, albeit at low frequency. These results suggested that G-CSF treatment initiated endogenous hepatic tissue regeneration in response to radiation injury and ameliorated its fibrogenic effects.
authors.affiliation: Henan Key Laboratory for Experimental Hematology, Henan Institute of Haematology, Henan Tumor Hospital, Zhengzhou University, 127 Dongming Road, Zhengzhou 450008, China.
authors.forename: N
authors.initials: N
authors.lastname: Li
authors.forename: L
authors.initials: L
authors.lastname: Zhang
authors.forename: H
authors.initials: H
authors.lastname: Li
authors.forename: B
authors.initials: B
authors.lastname: Fang
mesh_terms: D000418:Albumins / Q000235:genetics / Q000378:metabolism; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D016026:Bone Marrow Transplantation; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism; D005260:Female; D005455:Fluorescent Antibody Technique; D016179:Granulocyte Colony-Stimulating Factor / Q000008:administration & dosage*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007279:Injections, Subcutaneous; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects; D008103:Liver Cirrhosis / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D008115:Liver Regeneration / Q000187:drug effects; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D011833:Radiation Injuries, Experimental / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D011837:Radiation-Protective Agents / Q000008:administration & dosage*; D011994:Recombinant Proteins; D013997:Time Factors; D018183:Transplantation Chimera
chemical_list: D000418:Albumins; D015415:Biomarkers; D011837:Radiation-Protective Agents; D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor; D003094:Collagen