title: Transient neutropenia after granulocyte-colony stimulating factor administration is associated with neutrophil accumulation in pulmonary vasculature.
abstract: OBJECTIVE
  To better define the nature of the transient neutropenia shortly following granulocyte-colony stimulating factor (G-CSF) administration.
  
  
  METHODS
  To evaluate the disappearance of neutrophils, we investigated neutrophil trafficking. Ratios of neutrophil number to background cellularity for C57BL/6 LysM-EGFP knock-in mice and rhesus macaques were determined in the lung, liver, spleen, and kidney after G-CSF administration.
  
  
  RESULTS
  For the C57BL/6 LysM-EGFP knock-in mice, the enhanced green fluorescent protein expression (EGFP(+)) cells increased in the lung and spleen within 15 minutes of administering 50 μg/kg G-CSF subcutaneously, and continued to increase in the lung and spleen from 15 minutes to 30 minutes. At 240 minutes, the pulmonary infiltrate declined to a level comparable to the level at 15 minutes, while in the spleen EGFP(+) cells continued to increase. For rhesus macaques, CD18(+) cells also significantly increased in the lung 30 minutes after administration of 10 μg/kg G-CSF subcutaneously compared to the control level.
  
  
  CONCLUSIONS
  These results suggest that the transient neutropenia following G-CSF administration in the mouse and nonhuman primate is associated with an accumulation of neutrophils within pulmonary and splenic vasculature.
authors.affiliation: Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, 5 Research Court, Rockville, MD 20850, USA.
authors.forename: Catherine E
authors.initials: CE
authors.lastname: DeJesus
authors.forename: Jackson
authors.initials: J
authors.lastname: Egen
authors.forename: Mark
authors.initials: M
authors.lastname: Metzger
authors.forename: Xavier
authors.initials: X
authors.lastname: Alvarez
authors.forename: Christian A
authors.initials: CA
authors.lastname: Combs
authors.forename: Daniela
authors.initials: D
authors.lastname: Malide
authors.forename: Zu Xi
authors.initials: ZX
authors.lastname: Yu
authors.forename: Xin
authors.initials: X
authors.lastname: Tian
authors.forename: Robert E
authors.initials: RE
authors.lastname: Donahue
mesh_terms: D000818:Animals; D001772:Blood Cell Count; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D008168:Lung / Q000473:pathology*; D008253:Macaca mulatta; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009503:Neutropenia / Q000139:chemically induced* / Q000473:pathology*; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects*; D011652:Pulmonary Circulation
chemical_list: D016179:Granulocyte Colony-Stimulating Factor