title: The thyroid hormone responsive protein (THRP) has a critical role in the embryogenesis of Xenopus laevis.
abstract: The human and mouse homologs of the rat thyroid hormone responsive protein (THRP), c-abl-interacting protein 2 (Abi-2), are critically involved in neurological development. The Abi-2 gene is evolutionarily conserved in vertebrates, and is also found in Xenopus laevis and Drosophila melanogaster. The THRP gene is one of the few genes regulated by thyroid hormone in adult animals. Sequence analysis of the 5'-flanking region of the THRP gene identified a putative thyroid hormone response element (TRE) that is conserved between rat and human. To determine whether or not THRP regulates neural growth and development, THRP was constitutively expressed in transgenic X. laevis. Growth of most animals was halted in early neurulation while the few animals that survived the process developed into grossly malformed tadpoles. In contrast, control animals reached late embryonic stage 25. These observations suggest that THRP over-expression in early development is not compatible with completion of early embryogenesis and that a different strategy needs to be employed to investigate THRP function in this model.
authors.affiliation: Division of Endocrinology, Diabetes, and Metabolism, Department of Medicine, University of Florida College of Medicine, 653-1 West 8th Street-L14, Jacksonville, FL 32209, United States.
authors.forename: Wayland
authors.initials: W
authors.lastname: Lim
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Haas
authors.forename: Arshag D
authors.initials: AD
authors.lastname: Mooradian
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D047108:Embryonic Development / Q000235:genetics*; D006801:Humans; D051379:Mice; D009687:Nuclear Proteins / Q000235:genetics*; D011401:Promoter Regions, Genetic; D051381:Rats; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D014157:Transcription Factors / Q000235:genetics*; D014982:Xenopus laevis / Q000196:embryology*
chemical_list: D009687:Nuclear Proteins; C051065:THRSP protein, human; C501478:Thrsp protein, rat; D014157:Transcription Factors