title: Study of plasma metabolic profiling and biomarkers of chronic unpredictable mild stress rats based on gas chromatography/mass spectrometry.
abstract: A metabolomic investigation of chronic unpredictable mild stress (CUMS) rats was carried out. Plasma obtained from Sprague-Dawley (SD) rats treated by CUMS was analyzed using gas chromatography/mass spectrometry. Thirty-seven metabolites were identified among the detected compounds. Subsequent data analysis using the t test and principal component analysis (PCA) revealed significant metabolic changes in the rats' plasma after CUMS treatment. Clear separation between the model and control group was achieved, and the level of twelve metabolites, including amino acids, sugar, organic acids and fatty acids, were significantly different between plasma samples from the controls and CUMS group. These observations suggested that the depressed state may be associated with perturbation of amino acid metabolism, energy metabolism and glycometabolism. The study suggested that the metabolomics approach could be used as a potential powerful tool to investigate the biochemical change in certain physiopathological conditions, such as depression, as an early diagnostic means.
authors.affiliation: Modern Research Center for Traditional Chinese Medicine of Shanxi University, No.92 Wucheng Road, Taiyuan 030006, PR China.
authors.forename: Zhen-Yu
authors.initials: ZY
authors.lastname: Li
authors.forename: Xing-Yu
authors.initials: XY
authors.lastname: Zheng
authors.forename: Xiao-Xia
authors.initials: XX
authors.lastname: Gao
authors.forename: Yu-Zhi
authors.initials: YZ
authors.lastname: Zhou
authors.forename: Hai-Feng
authors.initials: HF
authors.lastname: Sun
authors.forename: Li-Zeng
authors.initials: LZ
authors.lastname: Zhang
authors.forename: Xiao-Qing
authors.initials: XQ
authors.lastname: Guo
authors.forename: Guan-Hua
authors.initials: GH
authors.lastname: Du
authors.forename: Xue-Mei
authors.initials: XM
authors.lastname: Qin
mesh_terms: D000596:Amino Acids / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D015415:Biomarkers / Q000097:blood; D001774:Blood Chemical Analysis / Q000379:methods*; D001835:Body Weight; D002264:Carboxylic Acids / Q000737:chemistry / Q000378:metabolism; D004355:Drug Stability; D004734:Energy Metabolism; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D054794:Glycomics / Q000379:methods*; D008297:Male; D055442:Metabolome; D055432:Metabolomics / Q000379:methods*; D025341:Principal Component Analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D013312:Stress, Physiological / Q000502:physiology*; D013395:Sucrose / Q000378:metabolism
chemical_list: D000596:Amino Acids; D015415:Biomarkers; D002264:Carboxylic Acids; D013395:Sucrose