title: Effects of pulp and paper mill effluent extracts on liver anaerobic and aerobic metabolic enzymes in rainbow trout.
abstract: This study investigates whether pulse exposure to Chilean pulp and paper mill effluent solid phase extracted (SPE) extracts via intraperitoneal injection (IP), would result in changes in the activities of the respiratory metabolic enzymes citrate synthase (CS) and lactate dehydrogenase (LDH) in rainbow trout livers. It also investigated if an alteration in liver metabolic capacity influenced the liver detoxification processes and estrogenic effects previously reported. Besides, a comparison of those enzymatic activities with fish IP injected with SPE extracts of two model effluents coming from industries that process 100% different type of feedstock (softwood, SW and hardwood, HW) was also evaluated. An initial induction of the anaerobic metabolism (increase in LDH enzymatic activity) was detected in all Chilean pulp mill effluent extracts evaluated, contrary to the initial unaltered aerobic metabolism (CS enzymatic activity) observed. A compensatory relationship in energy metabolism (Pasteur effect) was observed when comparing both enzymatic activities of fish exposed to those effluent extracts. LDH and CS activities observed in fish injected with Chilean extracts seem to be related to the effects observed in fish injected with SW extracts. This study showed that intraperitoneal injection of pulp and paper mill effluent extracts affected the anaerobic and aerobic metabolic capacities in rainbow trout livers, but this metabolic alteration did not affect detoxification capability or estrogenic effect previously reported.
authors.affiliation: Faculty of Science, University of Ontario Institute of Technology, 2000 Simcoe Street North, Oshawa, Ont. L1H 7K4, Canada.
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Orrego
authors.forename: Zacharias
authors.initials: Z
authors.lastname: Pandelides
authors.forename: John
authors.initials: J
authors.lastname: Guchardi
authors.forename: Douglas
authors.initials: D
authors.lastname: Holdway
mesh_terms: D000332:Aerobiosis; D000693:Anaerobiosis; D000818:Animals; D001141:Aromatase / Q000378:metabolism; D002950:Citrate (si)-Synthase / Q000378:metabolism; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism; D005618:Fresh Water / Q000737:chemistry; D008658:Inactivation, Metabolic; D007220:Industrial Waste / Q000009:adverse effects* / Q000032:analysis; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D017686:Oncorhynchus mykiss / Q000378:metabolism*; D010209:Paper; D052616:Solid Phase Extraction; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000378:metabolism / Q000633:toxicity*
chemical_list: D007220:Industrial Waste; D014874:Water Pollutants, Chemical; D007770:L-Lactate Dehydrogenase; D001141:Aromatase; D019363:Cytochrome P-450 CYP1A1; D002950:Citrate (si)-Synthase