title: Immobilized culture and transfection microarray of non-adherent cells.
abstract: Cell-based microarrays are promising tools for high-throughput functional analysis of gene products, but their application has been limited to adherent cells due to the difficulty in immobilization of non-adherent cells. Herein, we have introduced our techniques that can rapidly and strongly immobilize non-adherent cells and can allow the transfection of non-adherent cells with plasmid cDNA and small interfering RNA (siRNA) at a defined position on substrates with poly(ethylene-glycol)-lipid-modified surface.
authors.affiliation: Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, Tokyo, Japan. yamaguchi@bio.t.u-tokyo.ac.jp
authors.forename: Satoshi
authors.initials: S
authors.lastname: Yamaguchi
authors.forename: Erika
authors.initials: E
authors.lastname: Matsunuma
authors.forename: Teruyuki
authors.initials: T
authors.lastname: Nagamune
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D018914:Cells, Immobilized; D018076:DNA, Complementary / Q000235:genetics; D006801:Humans; D051379:Mice; D046228:Microarray Analysis / Q000379:methods*; D008856:Microscopy, Fluorescence; D010957:Plasmids; D011092:Polyethylene Glycols / Q000737:chemistry; D034741:RNA, Small Interfering / Q000235:genetics; D013379:Substrate Specificity; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D018076:DNA, Complementary; D034741:RNA, Small Interfering; D011092:Polyethylene Glycols