title: 'Young' oral fibroblasts are geno/phenotypically distinct.
abstract: Wound healing within the oral mucosa results in minimal scar formation compared with wounds within the skin. We have recently demonstrated distinct differences in the aging profiles of cells (oral mucosal and patient-matched skin fibroblasts) isolated from these tissues. We hypothesized that the increased replicative potential of oral mucosal fibroblasts may confer upon them preferential wound-healing capacities. Passage-matched early cultures of oral mucosal fibroblasts and skin fibroblasts demonstrated distinct gene expression profiles, with several genes linked to wound healing/tissue repair. This was related to an increased ability of the 'replicatively younger' oral mucosal fibroblasts to repopulate a wound space and reorganize their surrounding extracellular matrix environment, key activities during the wound-healing process. We conclude that oral mucosal fibroblasts exhibit a preferential healing response in vivo, due to their 'replicatively younger' phenotype when compared with that of patient-matched skin fibroblasts.
authors.affiliation: Wound Biology Group, Cardiff Institute of Tissue Engineering and Repair, Tissue Engineering and Reparative Dentistry, School of Dentistry, Cardiff University, Heath Park, Cardiff CF14 4XY, Wales, UK.
authors.forename: S
authors.initials: S
authors.lastname: Enoch
authors.forename: M A
authors.initials: MA
authors.lastname: Peake
authors.forename: I
authors.initials: I
authors.lastname: Wall
authors.forename: L
authors.initials: L
authors.lastname: Davies
authors.forename: J
authors.initials: J
authors.lastname: Farrier
authors.forename: P
authors.initials: P
authors.lastname: Giles
authors.forename: D
authors.initials: D
authors.lastname: Kipling
authors.forename: P
authors.initials: P
authors.lastname: Price
authors.forename: R
authors.initials: R
authors.lastname: Moseley
authors.forename: D
authors.initials: D
authors.lastname: Thomas
authors.forename: P
authors.initials: P
authors.lastname: Stephens
mesh_terms: D016022:Case-Control Studies; D002452:Cell Count; D018929:Cell Culture Techniques; D002453:Cell Cycle / Q000502:physiology; D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation; D016922:Cellular Senescence / Q000502:physiology; D003094:Collagen / Q000378:metabolism; D017077:Culture Media, Conditioned / Q000032:analysis; D004792:Enzyme Precursors / Q000032:analysis; D005109:Extracellular Matrix / Q000502:physiology; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology*; D018093:Gelatinases / Q000032:analysis; D020869:Gene Expression Profiling; D005838:Genotype; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000032:analysis; D046228:Microarray Analysis; D009061:Mouth Mucosa / Q000166:cytology* / Q000293:injuries; D010641:Phenotype; D012038:Regeneration / Q000235:genetics / Q000502:physiology; D012867:Skin / Q000166:cytology; D014945:Wound Healing / Q000235:genetics / Q000502:physiology
chemical_list: D017077:Culture Media, Conditioned; D004792:Enzyme Precursors; D003094:Collagen; D018093:Gelatinases; C069860:progelatinase; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2