title: Metabolic profiling of GuanXin II prescription based on metabolic fingerprinting and chemical analysis.
abstract: A sensitive LC/MS method was established to investigate the in vivo metabolism of GuanXin II prescription, a five-component Chinese herbal medicine formulation. Rat plasma, bile, urine, and feces were collected and analyzed following oral administration of the water decoction. A total of 50 compounds were identified, including 17 prototypes and 33 metabolites underwent methylation, oxidation, hydrolysis, sulfate conjugation, glucuronide conjugation, and glutathion conjugation. In addition, the component herb of the formulation from which the metabolites were derived was also identified. Among the five component herbs, Rhizoma Chuanxiong, Flos Carthami, and Lignum Dalbergiae Odoriferae were actively metabolized, contributing 26 metabolites and 2 prototypes, while Radix Salviae Miltiorrhizae and Radix Paeoniae Rubra underwent less biotransformation, yielding 7 metabolites and 15 prototypes. This is the first study on the metabolic profile of GuanXin II prescription. The results could be valuable to elucidate the material basis of this formulated Chinese medicine.
authors.affiliation: The State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Beijing, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Qiao
authors.forename: Jian
authors.initials: J
authors.lastname: Han
authors.forename: Man
authors.initials: M
authors.lastname: Xu
authors.forename: Zi-chuan
authors.initials: ZC
authors.lastname: Zhang
authors.forename: Min
authors.initials: M
authors.lastname: Ye
authors.forename: Guan
authors.initials: G
authors.lastname: Ye
authors.forename: De-an
authors.initials: DA
authors.lastname: Guo
mesh_terms: D000818:Animals; D001572:Benzofurans / Q000032:analysis* / Q000097:blood / Q000737:chemistry / Q000652:urine; D000081008:Bicyclic Monoterpenes; D001646:Bile / Q000737:chemistry; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D003324:Coronary Artery Disease / Q000188:drug therapy; D004365:Drugs, Chinese Herbal / Q000493:pharmacokinetics*; D005243:Feces / Q000737:chemistry; D005419:Flavonoids / Q000032:analysis* / Q000097:blood / Q000737:chemistry / Q000652:urine; D008297:Male; D015394:Molecular Structure; D010636:Phenols / Q000032:analysis* / Q000097:blood / Q000737:chemistry / Q000652:urine; D059808:Polyphenols; D051381:Rats; D017207:Rats, Sprague-Dawley; D052616:Solid Phase Extraction; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry; D013678:Technology, Pharmaceutical; D013729:Terpenes / Q000032:analysis* / Q000097:blood / Q000737:chemistry / Q000652:urine
chemical_list: D001572:Benzofurans; D000081008:Bicyclic Monoterpenes; D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D010636:Phenols; D059808:Polyphenols; D013729:Terpenes; C539574:guanxin No. 2; C030216:pinane; C082022:phthalide