title: [Biological effects of endostatin on vascular endothelial cell and fibroblast].
abstract: BACKGROUND
  To study the proliferation-inhibiting and apoptosis-inducing effects of recombinant human endostatin on both human umbilical vein endothelial cell ECV304 and fibroblast WI-38, and to investigate the efficacy, specificity and possible mechanisms of cell proliferation-inhibiting effect of endostatin.
  
  
  METHODS
  The different cells were cultured for a period of time in different kinds of culture fluids containing endostatin. The modality of the cells, proliferation-inhibiting rate and apoptosis degree were observed by MTT-cell proliferation detection and ELISA-cell death detection methods.
  
  
  RESULTS
  Recombinant human endostatin strikingly inhibited ECV304 cell proliferation in a dose-dependent manner, with an ED₅₀ value range from 15 to 20μg/ml, and it also strongly induced ECV304 cell apoptosis ( enrichment factor 2. 44) . No similar results were obtained in WI-38 cell.
  
  
  CONCLUSIONS
  Recombinant human endostatin can inhibit vascular endothelial cell proliferation specifically, and one of the possible mechanisms may be its apoptosis-inducing effect.
authors.affiliation: Department of Cardiothoracic Surgery , The General Hospital , Tianjin Medical University , Tianjin 300052, P . R. China.
authors.forename: P
authors.initials: P
authors.lastname: Zhang
authors.forename: J
authors.initials: J
authors.lastname: Yin
authors.forename: Y
authors.initials: Y
authors.lastname: Liu
authors.forename: W
authors.initials: W
authors.lastname: Zhang
authors.forename: X
authors.initials: X
authors.lastname: Su