title: [Antibacterial activity and mechanism of luteolin on Staphylococcus aureus].
abstract: OBJECTIVE
  To investigate the antibacterial activity and mechanism of luteolin on Staphylococcus aureus.
  
  
  METHODS
  The antibacterial activity and mechanism experiments were carried out by using 2, 3, 5-triphenyl tetrazolium chloride (TTC), determining membrane penetrability, the change of SDS-PAGE protein spectra and 4 6-diamidino-2-phenylindole (DAPI) staining assay.
  
  
  RESULTS
  Luteolin could affect the membrane permeability of Staphylococcus aureus, but not destroy the membrane integrity directly. After treated with luteolin for 16 hours, the total content of proteins decreased to 64.54%, the quantity of both DNA and RNA reduced to 48.44% and 39.35% respectively. The activity of DNA topoisomerase I and II was inhibited completely by 1.6 mg/mL luteolin.
  
  
  CONCLUSIONS
  Luteolin showed obvious antibacterial activity against Staphylococcus aureus. The antibacterial mechanism of luteolin is that it could inhibit the activity of DNA topoisomerase I and II, which resulted in some decrease in the nucleic acid and protein synthesis.
authors.affiliation: College of Life Science, Liaoning Normal University, Dalian 116029, China. wangqian19860223@163.com
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.forename: Mingjie
authors.initials: M
authors.lastname: Xie
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000378:metabolism; D002462:Cell Membrane / Q000187:drug effects; D047311:Luteolin / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D047311:Luteolin