title: Signal enhancement in DNA microarray using dye doped silica nanoparticles: application to human papilloma virus (HPV) detection.
abstract: DNA microarray is a powerful tool for the parallel of nucleic acids and other biologically significant molecules. In this communication we report an easy and cheap synthesis route for incorporating organic dyes into monodisperse inorganic silica nanoparticles and their application on the detection of carcinogenic risky Human Papilloma Virus using DNA microarray technology. We correlate our system with conventional direct dyes and commercial quantum dots, with a promising increase in optical signal, and a related decrease of the limit of detection, thus giving a remarkable improvement in this technique towards early diagnosis of diseases and trace level detection of dangerous biological contaminants.
authors.affiliation: CIVEN, via delle Industrie 5, 30175 Marghera, Venezia, Italy.
authors.forename: Raffaele
authors.initials: R
authors.lastname: Riccò
authors.forename: Anna
authors.initials: A
authors.lastname: Meneghello
authors.forename: Francesco
authors.initials: F
authors.lastname: Enrichi
mesh_terms: D052685:Alphapapillomavirus / Q000235:genetics* / Q000302:isolation & purification; D015374:Biosensing Techniques / Q000295:instrumentation; D004279:DNA, Viral / Q000032:analysis*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry*; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation*; D045663:Quantum Dots; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D004279:DNA, Viral; D005456:Fluorescent Dyes; D012822:Silicon Dioxide