title: Molecular cloning and characterization of caprine calpastatin gene.
abstract: Calpastatin (CAST) is an important gene for meat quality traits in livestock and poultry. The cDNA of caprine CAST gene was amplified for the first time using RACE-PCR. Results showed the full-length cDNA of caprine CAST gene (Accession no. GU944861) was 2435 base pair (bp) and contained a 2187 bp open reading frame encoding a protein with 728 amino acid residues. Bioinformatic analysis indicated that caprine CAST cDNA was 89.8-95.4, 83.5-92.2, 72.8-81.8 and 69.8-73.5% identical to sheep, cattle, pig and human CAST cDNA. It was predicted that caprine CAST contained four conserved domains with 42 serine phosphorylation loci, 18 threonine phosphorylation loci, 1 tyrosine phosphorylation locus and 5 specific PKC phosphorylation loci. This work provided an important experimental basis for further research on the function of CAST in goat.
authors.affiliation: College of Animal Science & Technology, Sichuan Agricultural University, 254 Zhang Jiashan Street, Ya'an, 625014, Sichuan, People's Republic of China.
authors.forename: Dai Hua
authors.initials: DH
authors.lastname: Wang
authors.forename: Gang Yi
authors.initials: GY
authors.lastname: Xu
authors.forename: Deng Jun
authors.initials: DJ
authors.lastname: Wu
authors.forename: Cheng Li
authors.initials: CL
authors.lastname: Zheng
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002135:Calcium-Binding Proteins / Q000737:chemistry / Q000235:genetics*; D002417:Cattle; D003001:Cloning, Molecular; D017124:Conserved Sequence / Q000235:genetics; D006041:Goats / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D005810:Multigene Family; D010455:Peptides / Q000737:chemistry; D010802:Phylogeny; D017434:Protein Structure, Tertiary; D013045:Species Specificity
chemical_list: D002135:Calcium-Binding Proteins; D010455:Peptides; C033668:calpastatin