title: Malt in combination with Lactobacillus rhamnosus increases concentrations of butyric acid in the distal colon and serum in rats compared with other barley products but decreases viable counts of cecal bifidobacteria.
abstract: Several substances, including glutamine and propionic acid but in particular butyric acid, have been proposed to be important for colonic health. β-Glucans lead to the formation of comparatively high amounts of butyric acid, and germinated barley foodstuff obtained from brewer's spent grain (BSG), containing high amounts of β-glucans and glutamine, has been reported to reduce the inflammatory response in the colon of patients with ulcerative colitis. The present study examines how 3 barley products, whole grain barley, malt, and BSG, affect SCFA in the hindgut and serum of rats and whether the addition of Lactobacillus rhamnosus 271 to each of these diets would have further effects. Amino acids in plasma and the cecal composition of the microbiota were also analyzed. The butyric acid concentration in the distal colon and serum was higher in the malt groups than in the other groups as was the serum concentration of propionic acid. The concentrations of propionic and butyric acids were higher in the cecum and serum of rats given L. rhamnosus than in those not given this strain. The proportion of plasma glutamine and the cecal number of bifidobacteria were lower in the malt groups than in the other groups. L. rhamnosus decreased the number of cecal bifidobacteria, whereas plasma glutamine was unaffected. We conclude that malt together with L. rhamnosus 271 had greater effects on propionic and butyric acid concentrations in rats than the other barley products. This is interesting when developing food with effects on colonic health.
authors.affiliation: Applied Nutrition and Food Chemistry, Department of Food Technology, Engineering and Nutrition, Center for Chemistry and Chemical Engineering, Lund University, SE-221 00 Lund, Sweden.
authors.forename: Camilla E
authors.initials: CE
authors.lastname: Bränning
authors.forename: Margareta E
authors.initials: ME
authors.lastname: Nyman
mesh_terms: D000818:Animals; D001644:Bifidobacterium / Q000302:isolation & purification*; D020148:Butyric Acid / Q000032:analysis* / Q000097:blood; D002432:Cecum / Q000382:microbiology*; D003106:Colon / Q000737:chemistry*; D004043:Dietary Fiber / Q000008:administration & dosage; D002523:Edible Grain; D005232:Fatty Acids, Volatile / Q000097:blood; D001467:Hordeum; D052201:Lacticaseibacillus rhamnosus; D008297:Male; D000644:Quaternary Ammonium Compounds / Q000097:blood; D051381:Rats; D017208:Rats, Wistar; D015430:Weight Gain
chemical_list: D004043:Dietary Fiber; D005232:Fatty Acids, Volatile; D000644:Quaternary Ammonium Compounds; D020148:Butyric Acid