title: Differentiation of AFS cells derived from the EGFP gene transgenic porcine fetuses.
abstract: We have obtained the EGFP (enhanced green fluorescence protein) gene transgenic porcine fetuses before. The aims of this study were (i) to determine whether stem cells could be isolated from amniotic fluid of the transgenic porcine fetuses, and (ii) to determine if these stem cells could express EGFP and differentiate in vitro. The results demonstrated that stem cells could be isolated from amniotic fluid of the EGFP gene transgenic porcine fetuses and could express EGFP and differentiate in vitro. Undifferentiated AFSs (amniotic fluid-derived stem cells) expressed POU5F1, THY1 and SOX2, while the following differentiation cells expressed markers for chondrogenic (COL2A1), osteogenic (osteocalcin and osteonectin) and neurogenic cells such as astrocyte (GFAP), oligodendrocyte (GALC) and neuron (NF, ENO2 and MAP).
authors.affiliation: College of Veterinary Medicine, Northwest AF University, Yangling, Shaanxi 712100, China. zhengyuemao@163.com
authors.forename: Yue-Mao
authors.initials: YM
authors.lastname: Zheng
authors.forename: Yong-Hui
authors.initials: YH
authors.lastname: Dang
authors.forename: Yong-Ping
authors.initials: YP
authors.lastname: Xu
authors.forename: Wu-Jia-Fu
authors.initials: WJ
authors.lastname: Sai
authors.forename: Zhi-Xing
authors.initials: ZX
authors.lastname: An
mesh_terms: D000653:Amniotic Fluid / Q000166:cytology* / Q000378:metabolism; D000818:Animals; D030801:Animals, Genetically Modified; D001253:Astrocytes / Q000166:cytology / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology*; D019902:Chondrocytes / Q000166:cytology / Q000378:metabolism; D024043:Collagen Type II / Q000096:biosynthesis; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism; D005333:Fetus / Q000166:cytology* / Q000378:metabolism; D005698:Galactosylceramidase / Q000096:biosynthesis; D049452:Green Fluorescent Proteins / Q000096:biosynthesis* / Q000235:genetics; D020928:Mitogen-Activated Protein Kinases / Q000096:biosynthesis; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D050814:Octamer Transcription Factor-3 / Q000096:biosynthesis; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism; D015675:Osteocalcin / Q000096:biosynthesis; D015676:Osteonectin / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055748:SOXB1 Transcription Factors / Q000096:biosynthesis; D013552:Swine; D018800:Thy-1 Antigens / Q000096:biosynthesis
chemical_list: D024043:Collagen Type II; D050814:Octamer Transcription Factor-3; D015676:Osteonectin; D055748:SOXB1 Transcription Factors; D018800:Thy-1 Antigens; C485184:enhanced green fluorescent protein; D015675:Osteocalcin; D049452:Green Fluorescent Proteins; D020928:Mitogen-Activated Protein Kinases; D005698:Galactosylceramidase