title: An organic solvent-, detergent-, and thermo-stable alkaline protease from the mesophilic, organic solvent-tolerant Bacillus licheniformis 3C5.
abstract: Bacillus licheniformis 3C5, isolated as mesophilic bacterium, exhibited tolerance towards a wide range of non-polar and polar organic solvents at 45 degrees C. It produced an extracellular organic solvent-stable protease with an apparent molecular mass of approximately 32 kDa. The inhibitory effect of PMSF and EDTA suggested it is likely to be an alkaline serine protease. The protease was active over abroad range of temperatures (45-70 degrees C) and pH (8-10) range with an optimum activity at pH 10 and 65 degrees C. It was comparatively stable in the presence ofa relatively high concentration (35% (v/v)) of organic solvents and various types of detergents even at a relatively high temperature (45 degrees C). The protease production by B. licheniformis 3C5 was growth-dependent. The optimization of carbon and nitrogen sources for cell growth and protease production revealed that yeast extract was an important medium component to support both cell growth and the protease production. The overall properties of the protease produced by B. licheniformis 3C5 suggested that this thermo-stable, solvent-stable, detergent-stable alkaline protease is a promising potential biocatalyst for industrial and environmental applications.
authors.affiliation: Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand.
authors.forename: W
authors.initials: W
authors.lastname: Rachadech
authors.forename: A
authors.initials: A
authors.lastname: Navacharoen
authors.forename: W
authors.initials: W
authors.lastname: Ruangsit
authors.forename: T
authors.initials: T
authors.lastname: Pongtharangkul
authors.forename: A S
authors.initials: AS
authors.lastname: Vangnai
mesh_terms: D001407:Bacillus / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development; D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D003470:Culture Media; D003902:Detergents / Q000737:chemistry / Q000494:pharmacology; D010450:Endopeptidases / Q000737:chemistry* / Q000378:metabolism; D004795:Enzyme Stability; D006358:Hot Temperature; D008970:Molecular Weight; D057057:Serine Proteases / Q000737:chemistry* / Q000378:metabolism; D012997:Solvents / Q000737:chemistry / Q000494:pharmacology
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D003902:Detergents; D012997:Solvents; D010450:Endopeptidases; D057057:Serine Proteases; C087822:alkaline protease