title: Human umbilical cord blood-derived stromal cells: a new resource in hematopoietic reconstitution in mouse haploidentical transplantation.
abstract: OBJECTIVE
  Our previous study showed that human umbilical cord blood-derived stromal cells (hUcBdSCs) expanded CD34(+) cells in vitro. This study further explored the role of hUcBdSCs in vivo.
  
  
  METHODS
  The cultured hUcBdSCs were infused into transplanted haploidentical mice to observe hematopoietic recovery and complications.
  
  
  RESULTS
  The engraftment was faster in transplantation with hUcBdSCs than without hUcBdSCs. The numbers of fibroblast (CFU-F), granulocyte/monocyte (CFU-GM), erythrocytic (CFU-E), and megakaryocyte (CFU-Mg) colony-forming units were greater among mice transplanted with hUcBdSCs than without hUcBdSCs. The scoring of graft-versus-host disease was significantly lower in mice that had been subjected to transplantation with hUcBdSCs than without hUcBdSCs. The infused hUcBdSCs migrated to the bone marrow of the recipients.
  
  
  CONCLUSIONS
  These data indicated that hUcBdSCs improved hematopoietic reconstitution in haploidentical transplantation in mice.
authors.affiliation: Department of Hematology, Xinqiao Hospital, Third Military Medical University, Chongqing, China.
authors.forename: C
authors.initials: C
authors.lastname: Zhang
authors.forename: X-H
authors.initials: XH
authors.lastname: Chen
authors.forename: X
authors.initials: X
authors.lastname: Zhang
authors.forename: L
authors.initials: L
authors.lastname: Gao
authors.forename: P Y
authors.initials: PY
authors.lastname: Kong
authors.forename: X G
authors.initials: XG
authors.lastname: Peng
authors.forename: X
authors.initials: X
authors.lastname: Liang
authors.forename: L
authors.initials: L
authors.lastname: Gao
authors.forename: Q Y
authors.initials: QY
authors.lastname: Wang
mesh_terms: D000818:Animals; D002465:Cell Movement; D002478:Cells, Cultured; D036101:Cord Blood Stem Cell Transplantation / Q000009:adverse effects; D005312:Fetal Blood / Q000166:cytology*; D005347:Fibroblasts / Q000637:transplantation; D006086:Graft vs Host Disease / Q000209:etiology / Q000517:prevention & control; D006410:Hematopoiesis; D018380:Hematopoietic Stem Cell Transplantation / Q000009:adverse effects; D006648:Histocompatibility; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D055153:Stem Cell Niche; D017154:Stromal Cells / Q000637:transplantation*; D013997:Time Factors; D018183:Transplantation Chimera