title: Respiration response imaging for real-time detection of microbial function at the single-cell level.
abstract: The ability to detect specific functions of uncultured microbial cells in complex natural communities remains one of the most difficult tasks of environmental microbiology. Here we present respiration response imaging (RRI) as a novel fluorescence microscopy-based approach for the identification of microbial function, such as the ability to use C(1) substrates, at a single-cell level. We demonstrate that RRI could be used for the investigation of heterogeneity of a single microbial population or for functional profiling of microbial cells from complex environmental communities, such as freshwater lake sediment.
authors.affiliation: Department of Chemical Engineering, University of Washington, Seattle, WA 98195, USA.
authors.forename: M C
authors.initials: MC
authors.lastname: Konopka
authors.forename: T J
authors.initials: TJ
authors.lastname: Strovas
authors.forename: David S
authors.initials: DS
authors.lastname: Ojala
authors.forename: L
authors.initials: L
authors.lastname: Chistoserdova
authors.forename: M E
authors.initials: ME
authors.lastname: Lidstrom
authors.forename: M G
authors.initials: MG
authors.lastname: Kalyuzhnaya
mesh_terms: D001419:Bacteria / Q000378:metabolism*; D004783:Environmental Microbiology; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D010084:Oxidation-Reduction; D010101:Oxygen Consumption