title: Isolation and properties of endothelial cells forming the blood-nerve barrier.
abstract: The blood-nerve barrier (BNB) is one of the functional barriers sheltering the nervous system from circulating blood. It is very important to understand the cellular properties of endothelial cells of endoneurial origin because these cells constitute the bulk of the BNB. This chapter describes a standard protocol for isolating the endothelial cells forming the BNB. In addition, methods for confirming some of the barrier properties of isolated endothelial cells are also described.
authors.affiliation: Department of Neurology and Clinical Neuroscience, Yamaguchi University Graduate School of Medicine, Ube, Japan.
authors.forename: Yasuteru
authors.initials: Y
authors.lastname: Sano
authors.forename: Takashi
authors.initials: T
authors.lastname: Kanda
mesh_terms: D000818:Animals; D049428:Blood-Nerve Barrier / Q000166:cytology* / Q000502:physiology; D002469:Cell Separation / Q000379:methods*; D042783:Endothelial Cells / Q000166:cytology* / Q000502:physiology*; D055806:Microvessels / Q000166:cytology / Q000502:physiology; D010525:Peripheral Nerves / Q000098:blood supply / Q000166:cytology / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar