title: Cloning and functional characterization of a chalcone isomerase from Trigonella foenum-graecum L.
abstract: Flavonoids belong to a group of plant natural products with variable phenolic structures and play important roles in protection against biotic and abiotic stress. Fenugreek (Trigonella foenum-graecum L.) seeds and stems contain flavonol glycosides and isoflavone derivatives. Up to now, the molecular features of fenugreek flavonoid biosynthesis have not been characterized. Here we present cloning of a cDNA encoding a chalcone isomerase (namely TFGCHI-1) from the leaves of T. foenum-graecum which convert chalcones to flavanones in vitro. Transformation of Arabidopsis loss-of-function TT5 (CHI) mutant with a TFGCHI-1 cDNA complemented TT5 and produced higher levels of flavonol glycosides than wild-type Col-0.
authors.affiliation: College of Plant Science, Jilin University, Changchun, China.
authors.forename: Jian-chun
authors.initials: JC
authors.lastname: Qin
authors.forename: Lin
authors.initials: L
authors.lastname: Zhu
authors.forename: Ming-jun
authors.initials: MJ
authors.lastname: Gao
authors.forename: Xian
authors.initials: X
authors.lastname: Wu
authors.forename: Hong-yu
authors.initials: HY
authors.lastname: Pan
authors.forename: Yan-sheng
authors.initials: YS
authors.lastname: Zhang
authors.forename: Xiang
authors.initials: X
authors.lastname: Li
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005419:Flavonoids / Q000096:biosynthesis; D005822:Genetic Vectors / Q000235:genetics; D006027:Glycosides / Q000096:biosynthesis; D019753:Intramolecular Lyases / Q000235:genetics* / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D012639:Seeds / Q000737:chemistry; D029910:Trigonella / Q000201:enzymology* / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D005419:Flavonoids; D006027:Glycosides; D010940:Plant Proteins; D019753:Intramolecular Lyases; C019865:chalcone isomerase