title: Gaining replicability in a nonhost compromises the silencing suppression activity of Tobacco mild green mosaic virus in a host.
abstract: Natural isolates of Tobacco mild green mosaic virus (TMGMV) fail to infect tomato because the tomato tm-1 protein binds to the replication proteins of TMGMV and prevents RNA replication. Previously, we isolated a TMGMV mutant that overcomes tm-1-mediated resistance and multiplies in tomato plants. Here, we show that the causal mutations in the replication protein gene that abolish the interaction with tm-1 reduce its ability to suppress RNA silencing in host plant Nicotiana benthamiana. The results suggest that the multifunctionality of the replication proteins is an evolutionary constraint of tobamoviruses that restricts their host ranges.
authors.affiliation: Plant-Microbe Interactions Research Unit, Division of Plant Sciences, National Institute of Agrobiological Sciences, Tsukuba 305-8602, Japan. ishika32@affrc.go.jp
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Ishibashi
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Meshi
authors.forename: Masayuki
authors.initials: M
authors.lastname: Ishikawa
mesh_terms: D058507:Host Specificity; D018551:Solanum lycopersicum / Q000821:virology*; D009154:Mutation; D010935:Plant Diseases / Q000821:virology; D034622:RNA Interference; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism; D014026:Nicotiana / Q000821:virology*; D017898:Tobamovirus / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism; D014774:Virulence; D014779:Virus Replication
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins