title: A G-CSF functionalized PLLA scaffold for wound repair: An in vitro preliminary study.
abstract: Targeting wound repair, we developed an electrospun poly-L-lactide fibrous scaffold functionalized with G-CSF, a growth factor which is widely recognized as important in wound healing homeostasis. The scaffold was characterized in terms of morphology, mechanical properties and in vitro capacity to induce organization of co-cultures of murine fibroblasts and keratinocytes into a dermo-epidermal multilayered structure. Our findings are consistent with the promotion of a nonhostile environment, in which seeded cells could arrange themselves in an appropriate topographic distribution of elements at different levels of maturation up to a cornified epithelium on the top layer, resembling native skin.
authors.affiliation: CIR - Area of Cardiovascular Surgery, University Campus Bio-Medico of Rome, 00128, Italy. c.spadaccio@unicampus.it
authors.forename: Cristiano
authors.initials: C
authors.lastname: Spadaccio
authors.forename: Alberto
authors.initials: A
authors.lastname: Rainer
authors.forename: Stefano
authors.initials: S
authors.lastname: De Porcellinis
authors.forename: Matteo
authors.initials: M
authors.lastname: Centola
authors.forename: Federico
authors.initials: F
authors.lastname: De Marco
authors.forename: Massimo
authors.initials: M
authors.lastname: Chello
authors.forename: Marcella
authors.initials: M
authors.lastname: Trombetta
authors.forename: Jorge A
authors.initials: JA
authors.lastname: Genovese
mesh_terms: D000818:Animals; D004343:Drug Implants / Q000138:chemical synthesis* / Q000494:pharmacology; D004867:Equipment Design; D019544:Equipment Failure Analysis; D016179:Granulocyte Colony-Stimulating Factor / Q000737:chemistry / Q000627:therapeutic use*; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000737:chemistry / Q000627:therapeutic use; D019344:Lactic Acid / Q000737:chemistry*; D010865:Pilot Projects; D011091:Polyesters; D011108:Polymers / Q000737:chemistry*; D054457:Tissue Scaffolds; D016896:Treatment Outcome; D014945:Wound Healing / Q000187:drug effects / Q000502:physiology; D014947:Wounds and Injuries / Q000628:therapy*
chemical_list: D004343:Drug Implants; D036341:Intercellular Signaling Peptides and Proteins; D011091:Polyesters; D011108:Polymers; D016179:Granulocyte Colony-Stimulating Factor; D019344:Lactic Acid; C033616:poly(lactide)