title: Magnetotactic bacteria penetration into multicellular tumor spheroids for targeted therapy.
abstract: Preliminary experiments showed that MC-1 magnetotactic bacteria (MTB) could be used for the delivery of therapeutic agents to tumoral lesions. Each bacterium can provide a significant thrust propulsion force generated by two flagella bundles exceeding 4pN. Furthermore, a chain of single-domain magnetosomes embedded in the cell allows computer directional control and tracking using a magnetic resonance imaging (MRI) system. Although these embedded functionalities suggest that MTB when under the influence of an external computer could be considered as biological microrobots with the potential of targeting tumors, little is known about their level of penetration in tumoral tissues. In this paper, in vitro experiments were performed to assess the capability of these bacteria to penetrate tumor tissue for the delivery of therapeutic agents. Multicellular tumor spheroids were used since they reproduce many properties of solid tumors. The results show the ability of these MTB when submitted to a directional magnetic field to penetrate inside a 3D multicellular tumor spheroid through openings present in the tissue.
authors.affiliation: NanoRobotics Laboratory, Department of Computer and Software Engineering, École Polytechnique de Montréal, (Québec) Canada, H3C 3A7. ouaijdi.felfoul@polymtl.ca
authors.forename: Nisryn
authors.initials: N
authors.lastname: Mokrani
authors.forename: Ouajdi
authors.initials: O
authors.lastname: Felfoul
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Afkhami Zarreh
authors.forename: Mahmood
authors.initials: M
authors.lastname: Mohammadi
authors.forename: Raquel
authors.initials: R
authors.lastname: Aloyz
authors.forename: Gerald
authors.initials: G
authors.lastname: Batist
authors.forename: Sylvain
authors.initials: S
authors.lastname: Martel
mesh_terms: D018407:Bacterial Physiological Phenomena; D066298:In Vitro Techniques; D008279:Magnetic Resonance Imaging; D008280:Magnetics; D009369:Neoplasms / Q000382:microbiology* / Q000473:pathology