title: A C/T mutation in microRNA target sites in BMP5 gene is potentially associated with fatness in pigs.
abstract: Bone morphogenetic protein 5 (BMP5) gene was suggested to be a potential positional candidate gene for fatness trait QTLs on SSC7. Here by comparative sequencing of BMP5 gene in Meishan (MS) and Large White (LW) pigs, one SNP *131C>T in 3' un-translated region was detected. Association analysis results in 322 LW×MS F(2) pigs showed that CC pigs had thinner back fatness and heavier ham than TC (P<0.05 or P<0.1), and had a higher fat percentage and a lower lean meat percentage (P<0.1) than TT. Moreover, this C/T transition was predicted to alter BMP5 interaction with let-7c and miR-184 by using RNA22 and RNAhybrid. The negative expression of BMP5 gene with let-7c and miR-184 detected from miRNAs overexpression in swine fibroblast, indicating these 2 miRNAs might participate in the translational inhibition of BMP5 gene. Overall, SNP *131C>T might be a good marker for fatness traits.
authors.affiliation: Key Laboratory of Pig Genetics and Breeding, Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, PR China.
authors.forename: G C
authors.initials: GC
authors.lastname: Shao
authors.forename: L F
authors.initials: LF
authors.lastname: Luo
authors.forename: S W
authors.initials: SW
authors.lastname: Jiang
authors.forename: C Y
authors.initials: CY
authors.lastname: Deng
authors.forename: Y Z
authors.initials: YZ
authors.lastname: Xiong
authors.forename: F E
authors.initials: FE
authors.lastname: Li
mesh_terms: D020413:3' Untranslated Regions; D050154:Adiposity / Q000235:genetics; D000465:Algorithms; D000818:Animals; D001665:Binding Sites; D001823:Body Composition / Q000235:genetics*; D055417:Bone Morphogenetic Protein 5 / Q000235:genetics* / Q000378:metabolism; D002478:Cells, Cultured; D005260:Female; D005347:Fibroblasts; D005787:Gene Frequency; D056726:Genetic Association Studies / Q000662:veterinary; D005819:Genetic Markers; D008297:Male; D035683:MicroRNAs / Q000378:metabolism*; D008956:Models, Chemical; D009154:Mutation; D020641:Polymorphism, Single Nucleotide; D040641:Quantitative Trait Loci / Q000235:genetics; D034421:Sus scrofa / Q000235:genetics* / Q000254:growth & development*
chemical_list: D020413:3' Untranslated Regions; D055417:Bone Morphogenetic Protein 5; D005819:Genetic Markers; D035683:MicroRNAs