title: HPLC-MS/MS shotgun proteomic research of deer antlers with multiparallel protein extraction methods.
abstract: Deer antlers mature rapidly in 60 days, and subsequently shed in 5 days with rapid ossification. During this procedure, the function of deer antlers changes significantly. Therefore, the profiling of antler proteome is helpful to discover important growing and shedding regulation proteins, which might be of great significance for studying development and regeneration. In this study, a parallel protein extraction strategy was developed to extract proteins from antlers of red deer with five different lysis solutions, followed by shotgun proteomic analysis by microflow reversed-phase liquid chromatography/electrospray ionization/tandem mass spectrometry (μRPLC-ESI-MS/MS) with a 30 cm-long serially coupled microcolumn. Our experimental results showed that the identified proteins extracted by five kinds of lysis solution were complementary to each other. In total, 416 unique proteins were identified, with relative molecular masses from 2000 to 600,000, and isoelectric points from 3.84 to 11.57. All these results demonstrate that the combination of parallel protein extraction strategy and μRPLC-ESI-MS/MS analysis with serially coupled long microcolumns might be of great significance for comprehensive proteomic research of deer antler.
authors.affiliation: Key Laboratory of Separation Science for Analytical Chemistry, National Chromatographic Research & Analysis Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Gao
authors.forename: Dingyin
authors.initials: D
authors.lastname: Tao
authors.forename: Yichu
authors.initials: Y
authors.lastname: Shan
authors.forename: Zhen
authors.initials: Z
authors.lastname: Liang
authors.forename: Lihua
authors.initials: L
authors.lastname: Zhang
authors.forename: Yushu
authors.initials: Y
authors.lastname: Huo
authors.forename: Yukui
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000999:Antlers / Q000737:chemistry* / Q000378:metabolism; D005591:Chemical Fractionation / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods; D003670:Deer; D010449:Peptide Mapping / Q000379:methods*; D011506:Proteins / Q000737:chemistry / Q000145:classification / Q000378:metabolism; D040901:Proteomics / Q000379:methods; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D013347:Subcellular Fractions; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D011506:Proteins