title: Malate dehydrogenase: a useful phylogenetic marker for the genus Aeromonas.
abstract: The reconstruction of correct genealogies among biological entities, the estimation of the divergence time between organisms or the study of the different events that occur along evolutionary lineages are not always based on suitable genes. For reliable results, it is necessary to look at full-length sequences of genes under stabilizing selection (neutral or purifying) and behaving as good molecular clocks. In bacteria it has been proved that the malate dehydrogenase gene (mdh) can be used to determine the inter- and intraspecies divergence, and hence this gene constitutes a potential marker for phylogeny and bacterial population genetics. We have sequenced the full-length mdh gene in 36 type and reference strains of Aeromonas. The species grouping obtained in the phylogenetic tree derived from mdh sequences was in agreement with that currently accepted for the genus Aeromonas. The maximum likelihood models applied to our sequences indicated that the mdh gene is highly conserved among the Aeromonas species and the main evolutionary force acting on it is purifying selection. Only two sites under potential diversifying selection were identified (T 108 and S 193). In order to determine if these two residues could have an influence on the MDH structure, we mapped them in a three-dimensional model constructed from the sequence of A. hydrophila using the human mitochondrial MDH as a template. The presence of purifying selection together with the linear relationship between substitutions and gene divergence makes the mdh an excellent candidate gene for a phylogeny of Aeromonas and probably for other bacterial groups.
authors.affiliation: Departament de Microbiologia i Parasitologia Sanitàries, Facultat de Farmàcia, Universitat de Barcelona, Avda. Joan XXIII s/n, 08028 Barcelona, Spain. mfarfan@ub.edu
authors.forename: Maribel
authors.initials: M
authors.lastname: Farfán
authors.forename: David
authors.initials: D
authors.lastname: Miñana-Galbis
authors.forename: Albert
authors.initials: A
authors.lastname: Garreta
authors.forename: J Gaspar
authors.initials: JG
authors.lastname: Lorén
authors.forename: M Carmen
authors.initials: MC
authors.lastname: Fusté
mesh_terms: D000333:Aeromonas / Q000145:classification* / Q000235:genetics; D020134:Catalytic Domain; D017124:Conserved Sequence; D005798:Genes, Bacterial; D005819:Genetic Markers / Q000235:genetics; D014644:Genetic Variation; D006801:Humans; D008291:Malate Dehydrogenase / Q000737:chemistry / Q000235:genetics*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010802:Phylogeny; D011487:Protein Conformation; D013698:Templates, Genetic
chemical_list: D005819:Genetic Markers; D008291:Malate Dehydrogenase