title: Development of a modified DNA extraction method for pulsed-field gel electrophoresis analysis of Staphylococcus aureus and enterococci without using lysostaphin.
abstract: A modified pulsed-field gel electrophoresis (PFGE) protocol was developed and applied to clinical isolates of Staphylococcus aureus and enterococci to reduce the cost of using lysostaphin. This protocol reduces the expenses of PFGE typing of S. aureus and enterococci as it removes the use of lysostaphin during the spheroplast formation from these bacteria.
authors.affiliation: Department for Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. mfeizabadi@tums.ac.ir
authors.forename: Mohammad Mehdi
authors.initials: MM
authors.lastname: Feizabadi
authors.forename: Arash
authors.initials: A
authors.lastname: Ghodousi
authors.forename: Bizhan
authors.initials: B
authors.lastname: Nomanpour
authors.forename: Maryam
authors.initials: M
authors.lastname: Omrani
authors.forename: Fereshteh
authors.initials: F
authors.lastname: Shahcheraghi
mesh_terms: D015373:Bacterial Typing Techniques / Q000191:economics / Q000379:methods*; D001431:Bacteriological Techniques / Q000191:economics / Q000379:methods*; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification*; D016521:Electrophoresis, Gel, Pulsed-Field / Q000191:economics / Q000379:methods*; D016983:Enterococcus / Q000145:classification / Q000235:genetics*; D006801:Humans; D008248:Lysostaphin / Q000191:economics / Q000378:metabolism; D013211:Staphylococcus aureus / Q000145:classification / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; D008248:Lysostaphin