title: Plasmodium falciparum spliceosomal RNAs: 3' and 5' end processing.
abstract: The major spliceosomal small nuclear ribonucleoproteins (snRNPs) consist of snRNA (U1, U2, U4 or U5) and several proteins which can be unique or common to each snRNP particle. The common proteins are known as Sm proteins; they are crucial for RNP assembly and nuclear import of spliceosomal RNPs. This paper reports detecting the interaction between Plasmodium falciparum snRNAs and Sm proteins, and the usual 5' trimethylated caps on the snRNAs, by immunoprecipitation with specific antibodies. Furthermore, an unusual poly(A) tail was detected on these non-coding RNAs.
authors.affiliation: Universidad Nacional de Colombia, Facultad de Ciencias, Laboratorio de Investigaciones Básicas en Bioquímica, Bogotá Colombia.
authors.forename: Calvo
authors.initials: C
authors.lastname: Eliana
authors.forename: Escobar
authors.initials: E
authors.lastname: Javier
authors.forename: Wasserman
authors.initials: W
authors.lastname: Moisés
mesh_terms: D000818:Animals; D030561:Databases, Nucleic Acid; D047468:Immunoprecipitation; D010963:Plasmodium falciparum / Q000235:genetics* / Q000378:metabolism*; D012313:RNA; D012342:RNA, Small Nuclear / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017381:Spliceosomes / Q000378:metabolism*
chemical_list: C034477:RNA primers; D012342:RNA, Small Nuclear; D012313:RNA