title: Inflammatory responses to acute pneumovirus infection in neonatal mice.
abstract: BACKGROUND
  The innate immune responses of neonates differ dramatically from those of adults. Here we examine the acute inflammatory responses of neonatal and weanling mice infected with pneumonia virus of mice (PVM), a rodent pathogen (family Paramyxoviridae, genus Pneumovirus) that replicates the sequelae of severe respiratory syncytial virus infection.
  
  
  RESULTS
  We demonstrate that virus replication proceeds indistinguishably in all age groups (inoculated at 1, 2, 3 and 4 weeks of age), although inflammatory responses vary in extent and character. Some of the biochemical mediators detected varied minimally with age at inoculation. Most of the mediators evaluated demonstrated elevated expression over baseline correlating directly with age at the time of virus inoculation. Among the latter group are CCL2, CCL3, and IFN-γ, all cytokines previously associated with PVM-induced inflammatory pathology in mature mice. Likewise, we detect neutrophil recruitment to lung tissue in all age groups, but recruitment is most pronounced among the older (3 - 4 week old) mice. Interestingly, all mice exhibit failure to thrive, lagging in expected weight gain for given age, including the youngest mice that present little overt evidence of inflammation.
  
  
  CONCLUSIONS
  Our findings among the youngest mice may explain in part the phenomenon of atypical or minimally symptomatic respiratory infections in human neonates, which may be explored further with this infection model.
authors.affiliation: Department of Pediatrics, SUNY Upstate Medical University, Syracuse, NY, USA.
authors.forename: Cynthia A
authors.initials: CA
authors.lastname: Bonville
authors.forename: Catherine
authors.initials: C
authors.lastname: Ptaschinski
authors.forename: Caroline M
authors.initials: CM
authors.lastname: Percopo
authors.forename: Helene F
authors.initials: HF
authors.lastname: Rosenberg
authors.forename: Joseph B
authors.initials: JB
authors.lastname: Domachowske
mesh_terms: D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D001835:Body Weight; D016207:Cytokines / Q000096:biosynthesis; D015870:Gene Expression; D008168:Lung / Q000276:immunology / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D029122:Murine pneumonia virus / Q000276:immunology* / Q000472:pathogenicity*; D009504:Neutrophils / Q000276:immunology; D018186:Pneumovirus Infections / Q000276:immunology / Q000473:pathology / Q000662:veterinary*; D014779:Virus Replication
chemical_list: D016207:Cytokines