title: Polymorphism Ala54Thr of fatty acid-binding protein 2: Allelic frequencies and influence on cardiovascular risk factors in a multicenter study of Castilla y Leon.
abstract: BACKGROUND
  A transition of guanin to alanin at codon 54 of the fatty acid-binding protein 2 gene (FABP2) results in an amino acid substitution (Ala54 to Thr54). This polymorphism was associated with some cardiovascular risk factors.
  
  
  OBJECTIVE
  The aim of our study was to investigate the influence of the Thr54 polymorphism in the FABP2 gene on obesity anthropometric parameters and cardiovascular risk factors in the fasted state in obese subjects and the allelic distribution of this polymorphism in a geographic area of Spain.
  
  
  METHODS
  A population of 264 obese subjects was analyzed in a cross-sectional study from all health centers of Castilla y Leon (Spain). A nutritional and biochemical evaluation was performed. The statistical analysis was performed for the combined Ala54/Thr54 and Thr54/Thr54 genotype as a dominant model.
  
  
  RESULTS
  The mean age was 41.1 ± 13.1 years and the mean BMI 36.5 ± 5.9, with 94 males (35.6%) and 170 females (74.4%). One hundred and fifty-three subjects (58%) had the genotype Ala54/Ala54 (wild-type group) and 111 (42%) participants had the genotype Ala54/Thr54 (n = 92, 34.8%) or Thr54/Thr54 (n = 19, 7.2%) (mutant-type group). The health area of Valladolid had a lower frequency of wild-type genotype and Ala54 allelic frequency than all the other health areas of Castilla y Leon. C-reactive protein was higher in the mutant-type than the wild-type group (3.4 ± 5.6 vs. 7.9 ± 10.4 mg/dl; p < 0.05).
  
  
  CONCLUSIONS
  The finding of this study is the association of the Thr54/Ala54 and Thr54/Thr54 FABP2 phenotypes with higher levels of C-reactive protein without relation to insulin resistance. Frequencies of this polymorphism are different among health areas of Castilla y Leon.
authors.affiliation: Institute of Endocrinology and Nutrition, Hospital Rio Hortega, University of Valladolid, Spain. dadluis@yahoo.es
authors.forename: D A
authors.initials: DA
authors.lastname: de Luis
authors.forename: M
authors.initials: M
authors.lastname: Ballesteros
authors.forename: E
authors.initials: E
authors.lastname: Ruiz
authors.forename: M
authors.initials: M
authors.lastname: Cordero
authors.forename: C
authors.initials: C
authors.lastname: Muñoz
authors.forename: M A
authors.initials: MA
authors.lastname: Penacho
authors.forename: P
authors.initials: P
authors.lastname: Iglesias
authors.forename: A
authors.initials: A
authors.lastname: Lopez Guzman
authors.forename: A
authors.initials: A
authors.lastname: Maldonado
authors.forename: L
authors.initials: L
authors.lastname: San Martin
authors.forename: V
authors.initials: V
authors.lastname: Puigdevall
authors.forename: E
authors.initials: E
authors.lastname: Romero
authors.forename: M
authors.initials: M
authors.lastname: Gonzalez Sagrado
authors.forename: O
authors.initials: O
authors.lastname: Izaola
authors.forename: R
authors.initials: R
authors.lastname: Conde
mesh_terms: D000328:Adult; D000409:Alanine / Q000235:genetics / Q000378:metabolism; D000886:Anthropometry; D015992:Body Mass Index; D002097:C-Reactive Protein / Q000032:analysis / Q000378:metabolism*; D002318:Cardiovascular Diseases / Q000097:blood / Q000453:epidemiology / Q000235:genetics*; D003430:Cross-Sectional Studies; D050556:Fatty Acid-Binding Proteins / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007333:Insulin Resistance / Q000235:genetics / Q000502:physiology; D008297:Male; D009154:Mutation; D009765:Obesity / Q000097:blood / Q000150:complications / Q000235:genetics*; D010641:Phenotype; D011110:Polymorphism, Genetic; D012307:Risk Factors; D013030:Spain; D013912:Threonine / Q000235:genetics / Q000378:metabolism
chemical_list: C493404:FABP2 protein, human; D050556:Fatty Acid-Binding Proteins; D013912:Threonine; D002097:C-Reactive Protein; D000409:Alanine