title: Effect of quercetin on kinetic properties of renal Na,K-ATPase in normotensive and hypertensive rats.
abstract: The effect of quercetin, a plant-derived bioflavonoid with documented positive effect on the cardiovascular system, was examined after 4-week supplementation in the dose of 20 mg kg(-1) x day(-1) to young male normotensive control (C) and to spontaneously hypertensive rats (SHR) over the period of their 5(th)-8(th) week of age. The study was focused on the influence of quercetin on properties of the renal Na,K-ATPase, a key system in maintaining the homeostasis of sodium in the organism. Spontaneous hypertension by itself enhanced the activity of Na,K-ATPase probably as a consequence of a higher number of active enzyme molecules, as suggested by the 15% increase of V(max), along with improved affinity to ATP, as indicated by the 30% decrease in the value of Michaelis-Menten constant K(m) in untreated SHR vs. untreated normotensive rats. Quercetin induced a decrease of Na,K-ATPase activity in the presence of all ATP and Na(+) concentrations investigated. Evaluation of kinetic parameters resulted in a constant V(max) value. The ATP-binding properties of the enzyme were not influenced by quercetin, as suggested by statistically insignificant changes in the value of K(m) both in controls and in SHR. On the other hand, the affinity to sodium decreased, as suggested by an increase in the K(Na) value by 22% and 31% in normotensive and hypertensive groups, respectively. This impairment in the affinity of the Na(+)-binding site of Na,K-ATPase molecules was probably responsible for the deteriorated enzyme function in the kidneys of quercetin treated animals.
authors.affiliation: Institute for Heart Research, Slovak Academy of Sciences, Bratislava, Slovakia.
authors.forename: L
authors.initials: L
authors.lastname: Mezesova
authors.forename: M
authors.initials: M
authors.lastname: Bartekova
authors.forename: V
authors.initials: V
authors.lastname: Javorkova
authors.forename: J
authors.initials: J
authors.lastname: Vlkovicova
authors.forename: A
authors.initials: A
authors.lastname: Breier
authors.forename: N
authors.initials: N
authors.lastname: Vrbjar
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001794:Blood Pressure; D001835:Body Weight / Q000187:drug effects; D004353:Drug Evaluation, Preclinical; D005419:Flavonoids / Q000494:pharmacology; D006973:Hypertension / Q000201:enzymology* / Q000503:physiopathology; D007668:Kidney / Q000187:drug effects* / Q000201:enzymology* / Q000378:metabolism; D007700:Kinetics; D008297:Male; D011794:Quercetin / Q000494:pharmacology*; D051381:Rats; D011918:Rats, Inbred SHR; D017208:Rats, Wistar; D012964:Sodium / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D011794:Quercetin; D012964:Sodium; D000254:Sodium-Potassium-Exchanging ATPase