title: Streptomyces turgidiscabies Car8 contains a modular pathogenicity island that shares virulence genes with other actinobacterial plant pathogens.
abstract: Streptomyces turgidiscabies Car8 is an actinobacterium that causes the economically important disease potato scab. Pathogenesis in this species is associated with a mobile pathogenicity island (PAISt) that site specifically inserts into the bacA gene in Streptomyces spp. Here we provide the 674,223 bp sequence of PAISt, which consists of two non-overlapping modules of 105,364 and 568,859 bp. These modules are delimited by three copies of an 8 bp palindromic sequence (TTCATGAA), that also is the integration site (att) of the element. Putative tyrosine recombinase (IntSt) and excisionase (XisSt) proteins are encoded just upstream of att-R. PAISt has regions of synteny to pathogenic, symbiotic and saprophytic actinomycetes. The 105,364 bp PAISt module is identical to a genomic island in Streptomyces scabies 87-22, while the 568,859 bp module contains only a short region of synteny to that genome. However, both modules contain previously characterized and candidate virulence genes.
authors.affiliation: Department of Plant Pathology and Plant-Microbe Biology, Cornell University, Ithaca, NY, USA.
authors.forename: José C
authors.initials: JC
authors.lastname: Huguet-Tapia
authors.forename: Jonathan H
authors.initials: JH
authors.lastname: Badger
authors.forename: Rosemary
authors.initials: R
authors.lastname: Loria
authors.forename: Gregg S
authors.initials: GS
authors.lastname: Pettis
mesh_terms: D001483:Base Sequence; D019295:Computational Biology; D004254:DNA Nucleotidyltransferases / Q000378:metabolism; D023061:Gene Order; D005798:Genes, Bacterial / Q000235:genetics; D044404:Genomic Islands; D019426:Integrases / Q000378:metabolism; D058977:Molecular Sequence Annotation; D008969:Molecular Sequence Data; D011995:Recombination, Genetic; D011198:Solanum tuberosum / Q000382:microbiology; D013302:Streptomyces / Q000201:enzymology / Q000235:genetics* / Q000472:pathogenicity*; D026801:Synteny / Q000235:genetics; D014443:Tyrosine / Q000378:metabolism; D014764:Viral Proteins / Q000378:metabolism; D014774:Virulence / Q000235:genetics*
chemical_list: D014764:Viral Proteins; D014443:Tyrosine; D004254:DNA Nucleotidyltransferases; D019426:Integrases; C063911:excisionase