title: Knock-in approaches.
abstract: Molecular genetic strategies to study gene function in mice or to generate a mouse model for a human disease are continuously under development. The application and importance of knock-in approaches are increasing. This chapter elaborates on novel developments for the generation of knock-in mice. Special emphasis is given to recombinase-mediated cassette exchange, a new emerging knock-in strategy that enables easy generation of a series of different knock-in mutations within one gene.
authors.affiliation: Laboratory for Experimental Mouse Genetics, Center for Human Genetics, K.U. Leuven, Leuven, Belgium. anton.roebroek@med.kuleuven.be
authors.forename: Anton J M
authors.initials: AJ
authors.lastname: Roebroek
authors.forename: Philip L S M
authors.initials: PL
authors.lastname: Gordts
authors.forename: Sara
authors.initials: S
authors.lastname: Reekmans
mesh_terms: D000483:Alleles; D000818:Animals; D004254:DNA Nucleotidyltransferases / Q000235:genetics / Q000378:metabolism; D055879:Gene Knock-In Techniques / Q000379:methods*; D051379:Mice; D008822:Mice, Transgenic; D008957:Models, Genetic; D016254:Mutagenesis, Insertional / Q000379:methods; D009154:Mutation
chemical_list: D004254:DNA Nucleotidyltransferases; C077570:Site-specific recombinase