title: Blood-brain barrier disruption in the treatment of brain tumors.
abstract: Standard chemotherapy administered systemically has a limited efficacy in the treatment of brain tumors. One of the major obstacles in the treatment of brain neoplasias is the impediment to delivery across the intact blood-brain barrier (BBB). Many innovative approaches have been developed to circumvent this obstacle. One such strategy is BBB disruption (BBBD), which successfully increases the delivery of antineoplastic agents to the central nervous system (CNS). This chapter describes the application of the BBBD technique in rats. Different methods to evaluate and measure BBB permeability following hyperosmolar mannitol infusion including Evans blue staining, albumin immunohistochemistry, and dynamic magnetic resonance imaging are also described.
authors.affiliation: Department of Neurosurgery, University of Sherbrooke Hospital, Sherbrooke, QC, Canada.
authors.forename: Marie
authors.initials: M
authors.lastname: Blanchette
authors.forename: David
authors.initials: D
authors.lastname: Fortin
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use*; D001812:Blood-Brain Barrier / Q000187:drug effects* / Q000378:metabolism* / Q000502:physiology; D001932:Brain Neoplasms / Q000175:diagnosis / Q000188:drug therapy*; D016503:Drug Delivery Systems; D005070:Evans Blue; D007150:Immunohistochemistry; D008279:Magnetic Resonance Imaging; D008297:Male; D010539:Permeability; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000970:Antineoplastic Agents; D005070:Evans Blue