title: Inhibition of penicillium digitatum and penicillium italicum in vitro and in planta with Panomycocin, a novel exo-β-1,3-glucanase isolated from Pichia anomala NCYC 434.
abstract: Panomycocin, a novel exo-beta 1,3 glucanase, was tested as an antifungal agent against green and blue mold diseases, the most important causes of post harvest decay in citrus fruits. All tested isolates of Penicillium digitatum and Penicillium italicum were susceptible to panomycocin in vitro. Effective panomycocin concentrations for 50% growth inhibition (MIC-2) for P. digitatum and P. italicum were 2 and 1 microg ml(-1), respectively. Complete (MIC-0) growth inhibition of all isolates observed at a panomycocin concentration of 16 microg ml(-1). Treatment of spores with panomycocin at values lower than the MIC-0 led to slower germ tube elongation and mycelium growth. In tests on fruit, panomycocin at concentrations equal to in vitro MIC-0 value protected lemon fruit from decay.
authors.affiliation: Department of Biology, TED Ankara College Foundation High School, Incek, Gölbaşı, 06830, Ankara, Turkey.
authors.forename: Demet Altınbay
authors.initials: DA
authors.lastname: Izgu
authors.forename: Remziye Aysun
authors.initials: RA
authors.lastname: Kepekci
authors.forename: Fatih
authors.initials: F
authors.lastname: Izgu
mesh_terms: D000935:Antifungal Agents / Q000302:isolation & purification / Q000494:pharmacology*; D002957:Citrus / Q000382:microbiology*; D006026:Glycoside Hydrolases / Q000302:isolation & purification / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability / Q000187:drug effects; D009183:Mycotoxins / Q000302:isolation & purification / Q000494:pharmacology*; D010407:Penicillium / Q000187:drug effects*; D010843:Pichia / Q000201:enzymology*; D013172:Spores, Fungal / Q000187:drug effects
chemical_list: D000935:Antifungal Agents; D009183:Mycotoxins; D006026:Glycoside Hydrolases; C526433:panomycocin protein, Pichia anomala