title: [Identification and biodiversity of yeasts from Qula in Tibet and milk cake in Yunnan of China].
abstract: OBJECTIVE
  To analyze the biodiversity and distribution of the yeast species of Qula in Tibet and milk cake in Yunnan, and to provide essential data for the utilization of yeasts in the traditional dairy products of China.
  
  
  METHODS
  Forty-one yeast strains were isolated from 5 samples of Qula in Tibet and 8 samples of milk cake in Yunnan. The isolates were identified by the large-subunit (26S) rDNA gene D1/D2 domain sequences analysis.
  
  
  RESULTS
  The population of yeast in Qula varied from 10(6) cfu/g to 10(7) cfu/g. The content of yeast ranged from 102 cfu/g to 10(6) cfu/g in milk cake. The average population of yeast in Qula was higher than milk cake for 34 folds. These strains were grouped in 12 species belonging to 10 genera. The dominant species in Qula were Pichia fermentans and Saccharomyces cerevisiae, but Candida zeylanoides and Pichia cactophila were major population in milk cake. The results showed that Pichia was the dominant genera both in Qula and milk cake.
  
  
  CONCLUSIONS
  There existed that yeasts of great biodiversity both of Qula in Tibet and milk cake in Yunnan, but quite different from each other.
authors.affiliation: Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Huhhot 010018, China. qingmanjun1985@126.com
authors.forename: Manjun
authors.initials: M
authors.lastname: Qing
authors.forename: Mei
authors.initials: M
authors.lastname: Bai
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.forename: Wenjun
authors.initials: W
authors.lastname: Liu
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Sun
authors.forename: Heping
authors.initials: H
authors.lastname: Zhang
authors.forename: Tiansong
authors.initials: T
authors.lastname: Sun
mesh_terms: D044822:Biodiversity; D002681:China; D003611:Dairy Products / Q000382:microbiology*; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D018609:Tibet; D015003:Yeasts / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*