title: High efficacy of anti DBL4ɛ-VAR2CSA antibodies in inhibition of CSA-binding Plasmodium falciparum-infected erythrocytes from pregnant women.
abstract: Malaria during pregnancy is a major cause of intra-uterine growth-retardation and infant death in sub-Saharan Africa. Ideally, this could be prevented by a vaccine delivered before the first pregnancy. Antibodies against domain DBL4ɛ from VAR2CSA has been shown to inhibit adhesion of laboratory isolates to the placental receptor chondroitin sulfate A. In this study, the binding inhibitory efficacy of IgG elicited by two different DBL4ɛ recombinant proteins was tested on a panel of fresh clinical isolates from pregnant women living in Benin and Tanzania. The most promising recombinant protein elicited antibodies with similar efficacy as pooled plasma from immune multi-gravid African women.
authors.affiliation: Centre for Medical Parasitology, Department of International Health, Immunology and Microbiology, University of Copenhagen, Denmark. pamelamagistrado@yahoo.com
authors.forename: Pamela A
authors.initials: PA
authors.lastname: Magistrado
authors.forename: Daniel
authors.initials: D
authors.lastname: Minja
authors.forename: Justin
authors.initials: J
authors.lastname: Doritchamou
authors.forename: Nicaise Tuikue
authors.initials: NT
authors.lastname: Ndam
authors.forename: Davis
authors.initials: D
authors.lastname: John
authors.forename: Christentze
authors.initials: C
authors.lastname: Schmiegelow
authors.forename: Achille
authors.initials: A
authors.lastname: Massougbodji
authors.forename: Madeleine
authors.initials: M
authors.lastname: Dahlbäck
authors.forename: Sisse B
authors.initials: SB
authors.lastname: Ditlev
authors.forename: Vera V
authors.initials: VV
authors.lastname: Pinto
authors.forename: Mafalda
authors.initials: M
authors.lastname: Resende
authors.forename: John
authors.initials: J
authors.lastname: Lusingu
authors.forename: Thor G
authors.initials: TG
authors.lastname: Theander
authors.forename: Ali
authors.initials: A
authors.lastname: Salanti
authors.forename: Morten A
authors.initials: MA
authors.lastname: Nielsen
mesh_terms: D000913:Antibodies, Protozoan / Q000276:immunology*; D000953:Antigens, Protozoan / Q000276:immunology*; D001541:Benin; D002448:Cell Adhesion; D045643:DNA Repair Enzymes / Q000378:metabolism*; D004912:Erythrocytes / Q000469:parasitology*; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D017780:Malaria Vaccines / Q000276:immunology; D016778:Malaria, Falciparum / Q000276:immunology*; D010963:Plasmodium falciparum / Q000276:immunology; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology; D013636:Tanzania; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; C095472:ERCC8 protein, human; D007074:Immunoglobulin G; D017780:Malaria Vaccines; D011994:Recombinant Proteins; D014157:Transcription Factors; C512601:VAR2CSA protein, Plasmodium falciparum; D045643:DNA Repair Enzymes