title: Waterjet dissection of peripheral nerves: an experimental study of the sciatic nerve of rats.
abstract: BACKGROUND
  Although waterjet dissection has been well evaluated in intracranial pathologies, little is known of its qualities in peripheral nerve surgery. Theoretically, the precise dissection qualities could support the separation of nerves from adjacent tissues and improve the preservation of nerve integrity in peripheral nerve surgery.
  
  
  OBJECTIVE
  To evaluate the potential of the new waterjet dissector in peripheral nerve surgery.
  
  
  METHODS
  Waterjet dissection with pressures of 20 to 80 bar was applied on the sciatic nerves of 101 rats. The effect of waterjet dissection on the sciatic nerve was evaluated by clinical tests, neurophysiological examinations, and histopathological studies up to 12 weeks after surgery.
  
  
  RESULTS
  With waterjet pressures up to 30 bar, the sciatic nerve was preserved in its integrity in all cases. Functional damaging was observed at pressures of 40 bar and higher. However, all but 1 rat in the 80 bar subgroup showed complete functional regeneration at 12 weeks after surgery. Histopathologically, small water bubbles were observed around the nerves. At 40 bar and higher, the sciatic nerves showed signs of direct nerve injury. However, all these animals showed nerve regeneration after 12 weeks, as demonstrated by histological studies.
  
  
  CONCLUSIONS
  Sciatic nerves were preserved functionally and morphologically at pressures up to 30 bar. Between 40 and 80 bar, reliable functional and morphological nerve regeneration occurred. Waterjet pressures up to 30 bar might be applied safely under clinical conditions. This technique might be well suited to separate intact peripheral nerves from adjacent tumor or scar tissue. Further studies will have to show the clinical relevance of these dissection qualities.
authors.affiliation: Neurochirurgische Klinik und Poliklinik, Universitätsmedizin, Johannes Gutenberg-Universität, Mainz, Germany.
authors.forename: Christoph A
authors.initials: CA
authors.lastname: Tschan
authors.forename: Doerthe
authors.initials: D
authors.lastname: Keiner
authors.forename: Harald D
authors.initials: HD
authors.lastname: Müller
authors.forename: Kerstin
authors.initials: K
authors.lastname: Schwabe
authors.forename: Michael R
authors.initials: MR
authors.lastname: Gaab
authors.forename: Joachim K
authors.initials: JK
authors.lastname: Krauss
authors.forename: Clemens
authors.initials: C
authors.lastname: Sommer
authors.forename: Joachim
authors.initials: J
authors.lastname: Oertel
mesh_terms: D000818:Animals; D004210:Dissection / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design / Q000379:methods; D007431:Intraoperative Complications / Q000517:prevention & control; D008297:Male; D008866:Microsurgery / Q000295:instrumentation / Q000379:methods; D023421:Models, Animal; D009416:Nerve Regeneration / Q000502:physiology; D019635:Neurosurgical Procedures / Q000295:instrumentation* / Q000379:methods; D011312:Pressure; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000601:surgery*; D020426:Sciatic Neuropathy / Q000503:physiopathology / Q000517:prevention & control; D013525:Surgical Instruments / Q000592:standards / Q000639:trends*; D007507:Therapeutic Irrigation / Q000592:standards / Q000639:trends*