title: Cell Sheet Stiffness Sensing without taking out from culture liquid.
abstract: Stiffness could be an important index for evaluating the vitality of cell sheet. This paper challenges the measurement of stiffness of transparent cell sheet in culture liquid without taking it out from petri dish. The system is composed of a micro air nozzle for supplying an air jet and a regular reflective type laser sensor for measuring the the deformation of transparent cell sheet. This system is called as Cell Sheet Stiffness Sensing system (CS(3) system). When an air jet is given to a cell sheet in culture liquid, it pushes away the liquid toward the outer direction at initial phase and reaches the surface of cell sheet. Without any switching motion, the air jet continuously imparts a force to the surface of cell sheet so that the sensor can measure the stiffness of the cell sheet.
authors.affiliation: Department of Mechanical Engineering, Osaka University, 2-1, Yamadaoka, Suita, Japan. uchida@hh.mech.eng.osaka-u.ac.jp
authors.forename: Ryohei
authors.initials: R
authors.lastname: Uchida
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Tanaka
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Higashimori
authors.forename: Kenjiro
authors.initials: K
authors.lastname: Tadakuma
authors.forename: Makoto
authors.initials: M
authors.lastname: Kaneko
authors.forename: Makoto
authors.initials: M
authors.lastname: Kondo
authors.forename: Masayuki
authors.initials: M
authors.lastname: Yamato
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003470:Culture Media; D055119:Elastic Modulus / Q000502:physiology; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007834:Lasers; D008846:Micromanipulation / Q000295:instrumentation*; D009061:Mouth Mucosa / Q000502:physiology*; D010783:Photometry / Q000295:instrumentation*; D011312:Pressure; D051381:Rats; D012996:Solutions
chemical_list: D003470:Culture Media; D012996:Solutions