title: Molecular typing and cdt genes prevalence of Campylobacter jejuni isolates from various sources.
abstract: The genetic diversity of 168 Campylobacter jejuni isolates originating from human (n=30), cattle (n=36), sheep (n=44), dog (n=35), and poultry (n=21) and cdt genes prevalence of the isolates were investigated. To determine the genetic diversity of these strains, random amplified polymorphic DNA-polymerase chain reaction (PCR) using a random primer (M13) was performed. The numbers of genotypes determined in human, cattle, sheep, dog, and poultry isolates were 19, 18, 17, 18, and 6, respectively. To find out the prevalence of cdt genes in C. jejuni isolates simultaneously, a multiplex PCR was performed. The prevalence of the separate cdt genes was found to vary from 69% to 100% for cdtA, 92% to 100% for cdtB, and 39% to 98% for cdtC. These rates without host discriminating were 95%, 98%, and 93% for cdtA, cdtB, and cdtC, respectively. The prevalence of all three cdt genes in strains originating from human, cattle, sheep, dog, and poultry were 87%, 67%, 84%, 89%, and 39%, respectively. These results showed the relatively high genetic heterogeneity and variation of cdt genes among C. jejuni isolates from various sources except for poultry isolates. This study gives baseline data on molecular characterization of C. jejuni strains from different sources.
authors.affiliation: Department of Microbiology, Faculty of Veterinary Medicine, University of Ondokuz Mayıs, 55139, Kurupelit, Samsun, Turkey.
authors.forename: Arzu
authors.initials: A
authors.lastname: Findik
authors.forename: Tuba
authors.initials: T
authors.lastname: Ica
authors.forename: Ertan Emek
authors.initials: EE
authors.lastname: Onuk
authors.forename: Duygu
authors.initials: D
authors.lastname: Percin
authors.forename: Tahsin Onur
authors.initials: TO
authors.lastname: Kevenk
authors.forename: Alper
authors.initials: A
authors.lastname: Ciftci
mesh_terms: D000818:Animals; D001427:Bacterial Toxins / Q000737:chemistry / Q000235:genetics*; D002169:Campylobacter Infections / Q000382:microbiology* / Q000662:veterinary; D016123:Campylobacter jejuni / Q000235:genetics*; D016001:Confidence Intervals; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000235:genetics; D014644:Genetic Variation; D006801:Humans; D019105:Random Amplified Polymorphic DNA Technique; D015203:Reproducibility of Results
chemical_list: D001427:Bacterial Toxins; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; C067044:cytolethal distending toxin