title: Porcine insulin receptor substrate 4 (IRS4) gene: cloning, polymorphism and association study.
abstract: Using PCR and inverse PCR techniques we obtained a 4,498 bp nucleotide sequence FN424076 encompassing the complete coding sequence of the porcine insulin receptor substrate 4 (IRS4) gene and its proximal promoter. The 1,269 amino acid porcine protein deduced from the nucleotide sequence shares 92% identity with the human IRS4 and possesses the same domains and the same number of tyrosine phosphorylation motifs as the human protein. We detected substitution FN424076:g.96C<G in the promoter region that segregates in Meishan and a synonymous substitution FN424076:g.1829T<C in the coding sequence with allele C present only in Meishan. Linkage mapping placed the IRS4 gene at position 82 cM on the current USDA-USMARC linkage map of porcine chromosome X. Association analyses were performed on 555 animals of 12th-15th generation of the Meishan × Large White cross and showed that both SNPs were highly significantly associated with backfat depth (P=0.0005) and that the SNP FN424076:g1829T<C was also associated with loin depth (P=0.017). The Meishan alleles increased back fat depth and decreased loin depth. IRS4 can be considered a positional candidate gene for at least some of the QTL located at the centromeric region of porcine chromosome X.
authors.affiliation: Institute of Animal Physiology and Genetics AS CR, v.v.i., Liběchov, Czech Republic.
authors.forename: Martin
authors.initials: M
authors.lastname: Masopust
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Vykoukalová
authors.forename: Aleš
authors.initials: A
authors.lastname: Knoll
authors.forename: Heinz
authors.initials: H
authors.lastname: Bartenschlager
authors.forename: Alan
authors.initials: A
authors.lastname: Mileham
authors.forename: Nader
authors.initials: N
authors.lastname: Deeb
authors.forename: Gary A
authors.initials: GA
authors.lastname: Rohrer
authors.forename: Stanislav
authors.initials: S
authors.lastname: Cepica
mesh_terms: D000818:Animals; D001483:Base Sequence; D001837:Body Weights and Measures; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D055106:Genome-Wide Association Study; D055504:Insulin Receptor Substrate Proteins / Q000235:genetics*; D016014:Linear Models; D008969:Molecular Sequence Data; D010641:Phenotype; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013552:Swine / Q000235:genetics*
chemical_list: D017931:DNA Primers; D055504:Insulin Receptor Substrate Proteins