title: A novel human derived cell-penetrating peptide in drug delivery.
abstract: Cell-penetrating peptides can carry a variety of biologically active molecules into cells. Here we have identified a novel CPP derived from the C-terminus of human extracellular superoxide dismutase (hC-SOD3) which was shown to be located throughout in the cytoplasm and nucleus by fluorescence microscopy investigation. Furthermore, when apoptin fused to hC-SOD3, it was translocated efficiently into HeLa cells resulting in antitumor activities. This study shows that hC-SOD3 has the potential to penetrate and translocate cargo molecules into cells and has no cytotoxicity at effective concentration.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Mailbox 365, 130 Meilong Road, Shanghai, 200237, People's Republic of China. zhaojian@ecust.edu.cn
authors.forename: Jian
authors.initials: J
authors.lastname: Zhao
authors.forename: Peng
authors.initials: P
authors.lastname: Gao
authors.forename: Wei
authors.initials: W
authors.lastname: Xiao
authors.forename: Li-Qiang
authors.initials: LQ
authors.lastname: Fan
authors.forename: Fu-Jun
authors.initials: FJ
authors.lastname: Wang
authors.forename: Su-Xia
authors.initials: SX
authors.lastname: Li
authors.forename: Jian-Wen
authors.initials: JW
authors.lastname: Liu
mesh_terms: D000818:Animals; D015153:Blotting, Western; D036022:Capsid Proteins / Q000378:metabolism; D057846:Cell-Penetrating Peptides / Q000235:genetics*; D016503:Drug Delivery Systems / Q000379:methods*; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D013482:Superoxide Dismutase / Q000235:genetics* / Q000378:metabolism
chemical_list: D036022:Capsid Proteins; D057846:Cell-Penetrating Peptides; C091717:VP3 protein, Chicken anemia virus; C441642:SOD3 protein, human; D013482:Superoxide Dismutase