title: [Identification and metabolism characterization of a Clostridium lituseburense strain isolated from high-altitude soil].
abstract: OBJECTIVE
  We studied the physiological, biochemical properties and metabolism of Clostridium lituseburense P4-1 from soil in Namucuo.
  
  
  METHODS
  We adopted Hungate anaerobic technique to get Strain P4-1 from soil in Namucuo. Through physiological, biochemical and phylogenetic analysis, we identified the strain P4-1.
  
  
  RESULTS
  Cells were Gram-positive and spore-forming. It grew between 13 and 40 degrees C (optimum at 37 degrees C), between pH value 5.0 and 10.O (optimum at 7.5-8.0), and with the presence of NaCl between 0%-5%. Strain P4-1 could metabolize many carbon sources including glucose, melibiose and mannitol. Metabolites of glucose were acetate, butyrate, propionate, CO2, and little H2. Based on 16S rDNA studies, strain P4-1 was most close to Clostridium lituseburense DSM 797 (M59107) with 98.7% similarity. Strain P4-1 could degrade p-toluene sulfonate.
  
  
  CONCLUSIONS
  Strain P4-1 tolerated low temperature, salt and could degrade p-toluene sulfonate. Its metabolites produced by fermentation of glucose could improve the soil micro-environment. It was significant for strain P4-1 to be utilized in the wastewater treatment.
authors.affiliation: Biogas Institute of Ministry of Agriculture, Key Laboratory of Energy Microbiology and Application, Chengdu 610041, China. luckilylq@yahoo.com.cn
authors.forename: Quanquan
authors.initials: Q
authors.lastname: Liu
authors.forename: Haichang
authors.initials: H
authors.lastname: Liu
authors.forename: Yu
authors.initials: Y
authors.lastname: Deng
authors.forename: Guoquan
authors.initials: G
authors.lastname: Hu
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000531:Altitude; D001482:Base Composition / Q000235:genetics; D003013:Clostridium / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology
chemical_list: D012336:RNA, Ribosomal, 16S