title: Dengue virus-induced autophagy regulates lipid metabolism.
abstract: Autophagy influences numerous cellular processes, including innate and adaptive immunity against intracellular pathogens. However, some viruses, including dengue virus (DENV), usurp autophagy to enhance their replication. The mechanism for a positive role of autophagy in DENV infection is unclear. We present data that DENV induction of autophagy regulates cellular lipid metabolism. DENV infection leads to an autophagy-dependent processing of lipid droplets and triglycerides to release free fatty acids. This results in an increase in cellular β-oxidation, which generates ATP. These processes are required for efficient DENV replication. Importantly, exogenous fatty acids can supplant the requirement of autophagy in DENV replication. These results define a role for autophagy in DENV infection and provide a mechanism by which viruses can alter cellular lipid metabolism to promote their replication.
authors.affiliation: Department of Microbiology, The University of Chicago, Chicago, IL 60637, USA.
authors.forename: Nicholas S
authors.initials: NS
authors.lastname: Heaton
authors.forename: Glenn
authors.initials: G
authors.lastname: Randall
mesh_terms: D000818:Animals; D001343:Autophagy / Q000502:physiology*; D002460:Cell Line; D006224:Cricetinae; D003716:Dengue Virus / Q000276:immunology / Q000472:pathogenicity* / Q000502:physiology; D005227:Fatty Acids / Q000378:metabolism*; D005786:Gene Expression Regulation; D022781:Hepatocytes / Q000821:virology*; D006801:Humans; D007668:Kidney / Q000166:cytology / Q000821:virology*; D050356:Lipid Metabolism; D014779:Virus Replication
chemical_list: D005227:Fatty Acids