title: Vibrio atlanticus sp. nov. and Vibrio artabrorum sp. nov., isolated from the clams Ruditapes philippinarum and Ruditapes decussatus.
abstract: Facultatively anaerobic marine bacteria isolated from cultured clams, Ruditapes decussatus and Ruditapes philippinarum, were previously investigated using amplified fragment length polymorphism (AFLP) and 16S rRNA gene sequence analyses. The isolates formed two AFLP clusters and belonged to the genus Vibrio, more precisely to the Splendidus clade. In this study, phylogenetic analyses based on sequences of the housekeeping genes rpoA, rpoD, pyrH, atpA and recA supported their inclusion in that clade forming two well differentiated groups with respect to the rest of the species within the clade, and confirmed that they formed two groups, separated from the rest of the species of the clade. DNA-DNA hybridization demonstrated that the isolates constitute two novel species of the genus Vibrio, which can be phenotypically differentiated from their closest relatives. The names Vibrio atlanticus sp. nov. and Vibrio artabrorum sp. nov. are proposed, with Vb 11.11(T) ( = CECT 7223(T)  = LMG 24300(T)) and Vb 11.8(T) ( = CECT 7226(T)  = LMG 23865(T)) as the type strains, respectively.
authors.affiliation: Departamento de Microbiología y Parasitología, Facultad de Biología. Universidad de Santiago de Compostela. 15782, Santiago de Compostela, Spain.
authors.forename: Ana L
authors.initials: AL
authors.lastname: Diéguez
authors.affiliation: Departamento de Microbiología y Parasitología, Facultad de Biología. Universidad de Santiago de Compostela. 15782, Santiago de Compostela, Spain.
authors.forename: Roxana
authors.initials: R
authors.lastname: Beaz-Hidalgo
authors.affiliation: BCCM/LMG Bacteria Collection, Laboratory of Microbiology, Ghent University, Ghent, Belgium.
authors.forename: Ilse
authors.initials: I
authors.lastname: Cleenwerck
authors.affiliation: Departamento de Microbiología y Parasitología, Facultad de Biología. Universidad de Santiago de Compostela. 15782, Santiago de Compostela, Spain.
authors.forename: Sabela
authors.initials: S
authors.lastname: Balboa
authors.affiliation: BCCM/LMG Bacteria Collection, Laboratory of Microbiology, Ghent University, Ghent, Belgium.
authors.forename: Paul
authors.initials: P
authors.lastname: de Vos
authors.affiliation: Departamento de Microbiología y Parasitología, Facultad de Biología. Universidad de Santiago de Compostela. 15782, Santiago de Compostela, Spain.
authors.forename: Jesús L
authors.initials: JL
authors.lastname: Romalde
mesh_terms: D000332:Aerobiosis; D000693:Anaerobiosis; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D049872:Bivalvia / Q000382:microbiology*; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D014733:Vibrio / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial