title: Uptake and utilization of arachidonic acid in infective larvae of Angiostrongylus cantonensis.
abstract: Infective larvae of Angiostrongylus cantonensis may take up and incorporate exogenous arachidonic acid into their lipid pool. By scintillation counting, uptake and incorporation were determined to be time dependent. Arachidonic acid was mainly incorporated into phospholipid (56.8%) and neutral lipid (22.4%) pools. In the neutral lipids, 64.0% was diglyceride and 36.0% triglyceride. Phosphatidylcholine was the predominant fatty acid in the phospholipid pool. In addition to the release of leukotriene B4, the parasite was found to generate radiolabelled CO2 after incubation with [U-14C]arachidonate. Moreover, enzymatic analysis of crude extracts revealed the presence of acyl-CoA dehydrogenase (short and long chain), thiolase, enoyl-CoA hydratase and 3-hydroxyacyl-CoA dehydrogenase. These findings suggest that infective larvae of A. cantonensis not only take up and incorporate exogenous arachidonic acid into their lipid pool, but may also utilize the fatty acid through a functional β-oxidation pathway.
authors.affiliation: Department of Parasitology, College of Medicine, Chang Gung University, Tao-Yuan 33332, Taiwan.
authors.forename: P
authors.initials: P
authors.lastname: Tang
authors.forename: L-C
authors.initials: LC
authors.lastname: Wang
mesh_terms: D015094:3-Hydroxyacyl CoA Dehydrogenases / Q000378:metabolism; D044944:Acyl-CoA Dehydrogenases / Q000378:metabolism; D017185:Angiostrongylus cantonensis / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism* / Q000472:pathogenicity*; D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism*; D001700:Biomphalaria / Q000469:parasitology*; D004075:Diglycerides / Q000378:metabolism; D004746:Enoyl-CoA Hydratase / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D007814:Larva / Q000378:metabolism; D010084:Oxidation-Reduction; D010743:Phospholipids / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D017206:Strongylida Infections / Q000469:parasitology*; D014280:Triglycerides / Q000378:metabolism
chemical_list: D004075:Diglycerides; D005227:Fatty Acids; D010743:Phospholipids; D014280:Triglycerides; D016718:Arachidonic Acid; D015094:3-Hydroxyacyl CoA Dehydrogenases; D044944:Acyl-CoA Dehydrogenases; D004746:Enoyl-CoA Hydratase