title: Nano-LC and HPLC-chip-ESI-MS: an emerging technique for glycobioanalysis.
abstract: In less than 5 years, an impressive number of methods based on nano-LC and HPLC-chip coupled online to MS were developed and implemented to comprehensively address structural heterogeneity of glycoconjugates and glycans in biological matrices. C18, graphitized carbon and amide-based stationary phases were adapted to nanoflow level and on chip format, leading to improved sensitivity of structural analysis and superior level of information on highly complex glycan and glycoconjugate mixtures. This review offers a summary of the recent progress in the application of nano-LC and HPLC-chip-MS in glycoanalytics of glycopeptides, glycoprotein glycans, glycosaminoglycans, oligosaccharides and glycosphingolipids.
authors.affiliation: Institute for Medical Physics and Biophysics, University of Münster, Robert Koch Straβe 31, D-48149 Münster, Germany.
authors.forename: Stephan
authors.initials: S
authors.lastname: Kirsch
authors.forename: Laura
authors.initials: L
authors.lastname: Bindila
mesh_terms: D000577:Amides / Q000737:chemistry; D002244:Carbon / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D006001:Glycoconjugates / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods*; D011134:Polysaccharides / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation / Q000379:methods*
chemical_list: D000577:Amides; D006001:Glycoconjugates; D011134:Polysaccharides; D002244:Carbon