title: Molecular and cellular characterization of expanded and cryopreserved human limbal epithelial stem cells reveal unique immunological properties.
abstract: Transplantation of ex vivo expanded autologous limbal stem cells into the diseased eye of patients with limbal stem cell deficiency (LSCD) has been in practice worldwide. However, isolation of limbal tissue from the normal eye of the patient with unilateral LSCD still remains a major concern for the donor. More importantly, autologous cell transplantation is not a viable option for patients with bilateral LSCD. The objective of the current study was to determine the expansion potential of human limbal epithelial stem cells (hLESCs) for their possible use in allo-transplantation. A total of six limbal biopsy samples were cultured and expanded in vitro up to passage level 1 (P-1), at which point the hLESCs were cryopreserved. Semi-quantitative RT-PCR and immunophenotypic analysis revealed that hLESCs obtained before and after cryopreservation retained the expression of major limbal epithelial stem cell markers such as p63, SSEA-4, ABCG2, cytokeratin 19 (CK19), integrin β1 and vimentin. One notable difference was that while P-0 hLESCs expressed HLA-DR mRNA, no HLA-DR gene expression was observed with the expanded and cryopreserved samples. Human LESCs did not express costimulatory proteins CD80 or B7-DC but expressed significant levels of CD86, B7-H1 and HLA-ABC molecules on the cell surface. Treatment of hLESCs with IFN-γ induced the expression of HLA-DR, indoleamine 2,3-dioxygenase (IDO) and HLA-G on these cells. Cultured hLESCs were unable to stimulate allogeneic T cell proliferation in vitro even in the presence of pro-inflammatory cytokine, IFN-γ. These results indicate that cryopreserved hLESCs are non-immunogenic in nature and express negative immunoregulatory molecules which may be critical for their survival in an allogeneic environment.
authors.affiliation: Regenerative Medicine Group, Reliance Life Sciences Pvt. Ltd., R-282, TTC Area of MIDC, Thane-Belapur Road, Rabale, Navi Mumbai 400 701, India. viraf_vasania@relbio.com
authors.forename: Viraf S
authors.initials: VS
authors.lastname: Vasania
authors.forename: Parvathy
authors.initials: P
authors.lastname: Prasad
authors.forename: Rajdeep Kaur
authors.initials: RK
authors.lastname: Gill
authors.forename: Ashish
authors.initials: A
authors.lastname: Mehta
authors.forename: Chandra
authors.initials: C
authors.lastname: Viswanathan
authors.forename: Shabari
authors.initials: S
authors.lastname: Sarang
authors.forename: Anish Sen
authors.initials: AS
authors.lastname: Majumdar
mesh_terms: D018929:Cell Culture Techniques; D015925:Cryopreservation; D003713:Dendritic Cells / Q000276:immunology; D004847:Epithelial Cells / Q000187:drug effects / Q000276:immunology*; D019573:Epithelium, Corneal; D005434:Flow Cytometry; D006680:HLA Antigens / Q000235:genetics; D006684:HLA-DR Antigens / Q000378:metabolism; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I / Q000235:genetics; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D016850:Limbus Corneae / Q000166:cytology*; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells / Q000187:drug effects / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D006680:HLA Antigens; D006684:HLA-DR Antigens; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I; D012333:RNA, Messenger; D007371:Interferon-gamma