title: High-throughput analyses of gene functions on a cell chip by electroporation.
abstract: Genome-wide functional annotation of genes is one of the major challenges in current biology. Such investigation requires a high-throughput methodology for efficient and parallel overexpression or silencing of multiplexed genes in living cells. The transfection method described here employs an electric pulse and a cell-chip technology, and provides the possibility of analyzing gene functions in a high-throughput manner.
authors.affiliation: Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan. kkato@frontier.kyoto-u.ac.jp
authors.forename: Koichi
authors.initials: K
authors.lastname: Kato
authors.forename: Hiroo
authors.initials: H
authors.lastname: Iwata
mesh_terms: D002461:Cell Line, Transformed; D018274:Electroporation / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry; D049452:Green Fluorescent Proteins / Q000235:genetics; D057809:HEK293 Cells; D057166:High-Throughput Screening Assays / Q000379:methods*; D006801:Humans; D008856:Microscopy, Fluorescence; D008970:Molecular Weight; D010957:Plasmids / Q000235:genetics; D011094:Polyethyleneimine / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D034741:RNA, Small Interfering / Q000235:genetics / Q000494:pharmacology; D012235:Rhodamines / Q000737:chemistry; D046888:Tissue Array Analysis / Q000379:methods*; D014162:Transfection
chemical_list: D005456:Fluorescent Dyes; D011108:Polymers; D034741:RNA, Small Interfering; D012235:Rhodamines; D049452:Green Fluorescent Proteins; D011094:Polyethyleneimine