title: Gene diagnosis of oculopharyngeal muscular dystrophy in a Chinese family by a GeneScan method.
abstract: This study aims to perform gene diagnosis for Chinese family patients with Oculopharyngeal muscular dystrophy (OPMD). Genomic DNAs were extracted from the pedigrees' members. Gene diagnosis was performed for these pedigrees' members by approaches, such as DNA sequencing and GeneScan. Sequence analysis and PABPN1 genotyping showed that the mutated allele in affected members of this family has nine trinucleotide repeats of GCG (GCG)(9), whereas the normal allele contains six trinucleotide repeats of GCG (GCG)(6). The above results suggest that mutated GCG repeats in PABPN1 gene may cause OPMD in this family, and PABPN1 genotyping could be used as a convenient, highly effective, and reliable gene diagnostic test for OPMD patients.
authors.affiliation: School of Life Sciences, Xiamen University, Xiamen, Fujian, China.
authors.forename: Pan
authors.initials: P
authors.lastname: You
authors.forename: Qilin
authors.initials: Q
authors.lastname: Ma
authors.forename: Tao
authors.initials: T
authors.lastname: Tao
mesh_terms: D000368:Aged; D002681:China; D004252:DNA Mutational Analysis; D005192:Family Health; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D025202:Molecular Diagnostic Techniques; D039141:Muscular Dystrophy, Oculopharyngeal / Q000175:diagnosis* / Q000235:genetics* / Q000503:physiopathology; D010375:Pedigree; D039122:Poly(A)-Binding Protein I / Q000235:genetics*; D011933:Reagent Kits, Diagnostic; D018911:Trinucleotide Repeats
chemical_list: D039122:Poly(A)-Binding Protein I; D011933:Reagent Kits, Diagnostic