title: BurkDiff: a real-time PCR allelic discrimination assay for Burkholderia pseudomallei and B. mallei.
abstract: A real-time PCR assay, BurkDiff, was designed to target a unique conserved region in the B. pseudomallei and B. mallei genomes containing a SNP that differentiates the two species. Sensitivity and specificity were assessed by screening BurkDiff across 469 isolates of B. pseudomallei, 49 isolates of B. mallei, and 390 isolates of clinically relevant non-target species. Concordance of results with traditional speciation methods and no cross-reactivity to non-target species show BurkDiff is a robust, highly validated assay for the detection and differentiation of B. pseudomallei and B. mallei.
authors.affiliation: Translational Genomics Research Institute, Flagstaff, Arizona, United States of America. jbowers@tgen.org
authors.forename: Jolene R
authors.initials: JR
authors.lastname: Bowers
authors.forename: David M
authors.initials: DM
authors.lastname: Engelthaler
authors.forename: Jennifer L
authors.initials: JL
authors.lastname: Ginther
authors.forename: Talima
authors.initials: T
authors.lastname: Pearson
authors.forename: Sharon J
authors.initials: SJ
authors.lastname: Peacock
authors.forename: Apichai
authors.initials: A
authors.lastname: Tuanyok
authors.forename: David M
authors.initials: DM
authors.lastname: Wagner
authors.forename: Bart J
authors.initials: BJ
authors.lastname: Currie
authors.forename: Paul S
authors.initials: PS
authors.lastname: Keim
mesh_terms: D000483:Alleles; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D042726:Burkholderia mallei / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D016957:Burkholderia pseudomallei / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics*; D003937:Diagnosis, Differential; D016680:Genome, Bacterial / Q000235:genetics; D005896:Glanders / Q000175:diagnosis / Q000382:microbiology; D006801:Humans; D008554:Melioidosis / Q000175:diagnosis / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D020641:Polymorphism, Single Nucleotide; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial