title: Concentrated diffusing colloidal probes of Ca2+-dependent cadherin interactions.
abstract: We report video microscopy measurements and inverse simulation analyses of specific Ca(2+)-dependent interactions between N-cadherin fragments attached to supported lipid bilayer-coated silica colloids in quasi-2D concentrated configurations. Our results include characterization of the bilayer formation and fluidity and the attachment of active extracellular cadherin fragments on bilayers. Direct measurements of interaction potentials show nonspecific macromolecular repulsion between cadherin fragments in the absence of Ca(2+) and irreversible bilayer fusion via cadherin-mediated attraction at >100 μM Ca(2+). Analysis of Ca(2+)-dependent N-cadherin bond formation in quasi-2D concentrated configurations using inverse Monte Carlo and Brownian Dynamics simulations show measurable attraction starting at 0.1 μM Ca(2+), a concentration significantly below previously reported values.
authors.affiliation: Chemical Engineering, Texas A&M University, College Station, Texas 77843, United States.
authors.forename: W Neil
authors.initials: WN
authors.lastname: Everett
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Beltran-Villegas
authors.forename: Michael A
authors.initials: MA
authors.lastname: Bevan
mesh_terms: D015820:Cadherins / Q000737:chemistry / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D003102:Colloids; D004058:Diffusion; D006801:Humans; D008051:Lipid Bilayers / Q000737:chemistry / Q000378:metabolism; D008560:Membrane Fluidity; D018613:Microscopy, Confocal; D008968:Molecular Conformation; D056004:Molecular Dynamics Simulation; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding; D012822:Silicon Dioxide / Q000737:chemistry*; D013379:Substrate Specificity; D013499:Surface Properties
chemical_list: D015820:Cadherins; D003102:Colloids; D008051:Lipid Bilayers; D010446:Peptide Fragments; D012822:Silicon Dioxide; D002118:Calcium