title: Spectroscopic characterization and antiproliferative activity on HepG2 human hepatoblastoma cells of flavonoid C-glycosides from Petrorhagia velutina.
abstract: Eight flavonoid C-glycosides, including three new analogues, have been isolated from leaf and root methanolic extracts of Petrorhagia velutina, a Mediterranean herbaceous plant. The antiproliferative activity against human hepatoblastoma cancer cell line HepG2 has been analyzed by the MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide) test. Isoorientin (4) significantly reduces the proliferation of HepG2 cells as determined by the complete conversion of the tetrazolium probe into formazan after 48 h of exposure.
authors.affiliation: Laboratorio di Fitochimica, Dipartimento di Scienze della Vita, Seconda Università degli Studi di Napoli, Via Vivaldi 43, Caserta, Italy.
authors.forename: Severina
authors.initials: S
authors.lastname: Pacifico
authors.forename: Monica
authors.initials: M
authors.lastname: Scognamiglio
authors.forename: Brigida
authors.initials: B
authors.lastname: D'Abrosca
authors.forename: Simona
authors.initials: S
authors.lastname: Piccolella
authors.forename: Nikolaos
authors.initials: N
authors.lastname: Tsafantakis
authors.forename: Marialuisa
authors.initials: M
authors.lastname: Gallicchio
authors.forename: Andreina
authors.initials: A
authors.lastname: Ricci
authors.forename: Antonio
authors.initials: A
authors.lastname: Fiorentino
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D029748:Caryophyllaceae / Q000737:chemistry*; D004354:Drug Screening Assays, Antitumor; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D008401:Gas Chromatography-Mass Spectrometry; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D056945:Hep G2 Cells; D006801:Humans; D007558:Italy; D047311:Luteolin / Q000494:pharmacology; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D018515:Plant Leaves / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D013778:Tetrazolium Salts / Q000494:pharmacology; D013844:Thiazoles / Q000494:pharmacology
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D005419:Flavonoids; D006027:Glycosides; D013778:Tetrazolium Salts; D013844:Thiazoles; C057912:homoorientin; C022616:thiazolyl blue; D047311:Luteolin