title: Development of a validated high-throughput LC-ESI-MS method for determination of sirolimus on dried blood spots.
abstract: A high-throughput liquid chromatography-electrospray ionization mass spectrometric (LC-ESI-MS) method for screening of sirolimus on dried blood spots (DBS) was developed and validated. It involves solvent extraction of a punch of DBS followed by reversed-phase LC on a relatively new monolithic column consisting of a silica rod with bimodal pore structure and detection by ESI-MS. The run time was less than 3 min with a very low backpressure at a flow rate of 0.5 mL/min. The method can analyze more than 100 samples in an 8 h working day, including sample preparation. The assay was linear from 1 to 100 ng/mL. The mean recovery was 92.42%. The mean inter-day and intra-day precisions were 1.23 and 1.41%, respectively. The developed method is simple, rapid and useful for clinical applications.
authors.affiliation: HPLC Group, Analytical Chemistry Division, IICT, Tarnaka, Hyderabad-500007, India. rnrao55@yahoo.com
authors.forename: R Nageswara
authors.initials: RN
authors.lastname: Rao
authors.forename: Pawan K
authors.initials: PK
authors.lastname: Maurya
authors.forename: M
authors.initials: M
authors.lastname: Ramesh
authors.forename: R
authors.initials: R
authors.lastname: Srinivas
authors.forename: Sachin B
authors.initials: SB
authors.lastname: Agwane
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D057166:High-Throughput Screening Assays / Q000379:methods*; D006801:Humans; D020123:Sirolimus / Q000097:blood*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D020123:Sirolimus