title: Quantiferon-CMV test in prediction of cytomegalovirus infection after kidney transplantation.
abstract: Infection with cytomegalovirus (CMV) is a major cause of morbidity and mortality in immunosuppressed patients, including organ and bone marrow transplant recipients. The majority of CMV disease is caused by reactivation of alatent infection rather that by newly acquired virus. Many techniques have been currently available to aid in the diagnostics of CMV disease. In this report we performed a prospective evaluation of Quantiferon-CMV assay (Cellestis) to determine whether the test is predictive of CMV disease. CD8+ T-cell CMV-specific immunity was assessed in a longitudinal cohort of 14 kidney transplant recipients. According to our data, subjects with higher cellular immune response measured with Quantiferon test had a lower risk of manifestation of CMV infection than subjects with lower responses. Despite the small number of patients and large intra- and interindividual variability of the data in the study, we observed the Quantiferon-CMV assay to be a sensitive specific test to detect a virus-specific T-cell response. We propose that this assay in combination with viral DNA load estimates may prove to be useful to stratify patients at risk of CMV disease.
authors.affiliation: Department of Immunology and Allergy, Institute of Public Health, Partyzanske nam. 7, Ostrava 702 00, Czech Republic. alexandra.lochmanova@zuova.cz
authors.forename: A
authors.initials: A
authors.lastname: Lochmanova
authors.forename: I
authors.initials: I
authors.lastname: Lochman
authors.forename: H
authors.initials: H
authors.lastname: Tomaskova
authors.forename: P
authors.initials: P
authors.lastname: Marsalkova
authors.forename: J
authors.initials: J
authors.lastname: Raszka
authors.forename: J
authors.initials: J
authors.lastname: Mrazek
authors.forename: J
authors.initials: J
authors.lastname: Dedochova
authors.forename: A
authors.initials: A
authors.lastname: Martinek
authors.forename: H
authors.initials: H
authors.lastname: Brozmanova
authors.forename: M
authors.initials: M
authors.lastname: Grundmann
mesh_terms: D000328:Adult; D000368:Aged; D000914:Antibodies, Viral / Q000097:blood; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000821:virology*; D003587:Cytomegalovirus / Q000235:genetics / Q000276:immunology*; D003586:Cytomegalovirus Infections / Q000175:diagnosis* / Q000209:etiology / Q000276:immunology; D018153:Czech Republic; D004279:DNA, Viral / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007111:Immunity, Cellular; D007166:Immunosuppressive Agents / Q000009:adverse effects*; D007371:Interferon-gamma / Q000097:blood; D016030:Kidney Transplantation / Q000009:adverse effects*; D008137:Longitudinal Studies; D008297:Male; D008875:Middle Aged; D011237:Predictive Value of Tests; D011446:Prospective Studies; D015203:Reproducibility of Results; D018570:Risk Assessment; D012680:Sensitivity and Specificity; D016896:Treatment Outcome; D019562:Viral Load; D017735:Virus Latency; D055815:Young Adult
chemical_list: D000914:Antibodies, Viral; D004279:DNA, Viral; D007166:Immunosuppressive Agents; D007371:Interferon-gamma