title: 2D immunomic approach for the study of IgG autoantibodies in the experimental model of multiple sclerosis.
abstract: 2D-immunomics may be useful in the identification of autoantigens in neurological autoimmune diseases, but its application may be limited by denaturation of target proteins. Here we compared the capacity of a single or multiple antigens to elicit autoantibodies targeting multiple neural autoantigens by ELISA and 2D-immunomics. We induced experimental autoimmune encephalomyelitis (EAE) with MBP peptide(89-104), total MBP or spinal cord homogenate. Both techniques showed anti-MBP IgG only after immunization with total MBP. In addition, 2D-immunomics revealed the presence in EAE mice of autoantibodies targeting other neural proteins, some displaying partial sequence homology with MBP. The present finding by 2D-immunomics of multiple neural proteins targeted by autoantibodies generated by a single antigen may help to explain the complex autoimmune response observed in multiple sclerosis.
authors.affiliation: Department of Neuroscience, University of Verona, Italy.
authors.forename: Alessia
authors.initials: A
authors.lastname: Farinazzo
authors.forename: Beatrice
authors.initials: B
authors.lastname: Gini
authors.forename: Alberto
authors.initials: A
authors.lastname: Milli
authors.forename: Francesca
authors.initials: F
authors.lastname: Ruffini
authors.forename: Silvia
authors.initials: S
authors.lastname: Marconi
authors.forename: Ermanna
authors.initials: E
authors.lastname: Turano
authors.forename: Elena
authors.initials: E
authors.lastname: Anghileri
authors.forename: Francesca
authors.initials: F
authors.lastname: Barbieri
authors.forename: Daniela
authors.initials: D
authors.lastname: Cecconi
authors.forename: Roberto
authors.initials: R
authors.lastname: Furlan
authors.forename: Bruno
authors.initials: B
authors.lastname: Bonetti
mesh_terms: D000818:Animals; D001323:Autoantibodies / Q000032:analysis* / Q000276:immunology; D001324:Autoantigens / Q000276:immunology; D002851:Chromatography, High Pressure Liquid; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007091:Image Processing, Computer-Assisted; D015151:Immunoblotting / Q000379:methods*; D007074:Immunoglobulin G / Q000032:analysis* / Q000276:immunology; D013058:Mass Spectrometry; D051379:Mice; D009103:Multiple Sclerosis / Q000276:immunology; D004676:Myelin Basic Protein / Q000276:immunology; D010446:Peptide Fragments / Q000276:immunology; D013116:Spinal Cord / Q000276:immunology / Q000473:pathology
chemical_list: D001323:Autoantibodies; D001324:Autoantigens; D007074:Immunoglobulin G; D004676:Myelin Basic Protein; D010446:Peptide Fragments