title: Mining the plant-herbivore interface with a leafmining Drosophila of Arabidopsis.
abstract: Experimental infections of Arabidopsis thaliana (Arabidopsis) with genomically characterized plant pathogens such as Pseudomonas syringae have facilitated the dissection of canonical eukaryotic defence pathways and parasite virulence factors. Plants are also attacked by herbivorous insects, and the development of an ecologically relevant genetic model herbivore that feeds on Arabidopsis will enable the parallel dissection of host defence and reciprocal resistance pathways such as those involved in xenobiotic metabolism. An ideal candidate is Scaptomyza flava, a drosophilid fly whose leafmining larvae are true herbivores that can be found in nature feeding on Arabidopsis and other crucifers. Here, we describe the life cycle of S. flava on Arabidopsis and use multiple approaches to characterize the response of Arabidopsis to S. flava attack. Oviposition choice tests and growth performance assays on different Arabidopsis ecotypes, defence-related mutants, and hormone and chitin-treated plants revealed significant differences in host preference and variation in larval performance across Arabidopsis accessions. The jasmonate and glucosinolate pathways in Arabidopsis are important in mediating quantitative resistance against S. flava, and priming with jasmonate or chitin resulted in increased resistance. Expression of xenobiotic detoxification genes was reduced in S. flava larvae reared on Arabidopsis jasmonate signalling mutants and increased in plants pretreated with chitin. These results and future research directions are discussed in the context of developing a genetic model system to analyse insect-plant interactions.
authors.affiliation: Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA 02478, USA. whiteman@email.arizona.edu
authors.forename: Noah K
authors.initials: NK
authors.lastname: Whiteman
authors.forename: Simon C
authors.initials: SC
authors.lastname: Groen
authors.forename: Daniela
authors.initials: D
authors.lastname: Chevasco
authors.forename: Ashley
authors.initials: A
authors.lastname: Bear
authors.forename: Noor
authors.initials: N
authors.lastname: Beckwith
authors.forename: T Ryan
authors.initials: TR
authors.lastname: Gregory
authors.forename: Carine
authors.initials: C
authors.lastname: Denoux
authors.forename: Nicole
authors.initials: N
authors.lastname: Mammarella
authors.forename: Frederick M
authors.initials: FM
authors.lastname: Ausubel
authors.forename: Naomi E
authors.initials: NE
authors.lastname: Pierce
mesh_terms: D000818:Animals; D017360:Arabidopsis / Q000235:genetics / Q000276:immunology / Q000469:parasitology*; D002686:Chitin / Q000494:pharmacology; D003517:Cyclopentanes / Q000494:pharmacology; D018428:Drosophilidae / Q000235:genetics / Q000502:physiology*; D005260:Female; D018506:Gene Expression Regulation, Plant; D017344:Genes, Insect; D049750:Genome, Insect; D005961:Glucosinolates / Q000378:metabolism; D007113:Immunity, Innate; D007814:Larva / Q000254:growth & development; D009154:Mutation; D010058:Oviposition; D054883:Oxylipins / Q000494:pharmacology; D010802:Phylogeny; D030821:Plants, Genetically Modified / Q000235:genetics / Q000276:immunology / Q000469:parasitology; D011401:Promoter Regions, Genetic; D017382:Reactive Oxygen Species / Q000378:metabolism; D015262:Xenobiotics / Q000494:pharmacology
chemical_list: D003517:Cyclopentanes; D005961:Glucosinolates; D054883:Oxylipins; D017382:Reactive Oxygen Species; D015262:Xenobiotics; D002686:Chitin; C011006:jasmonic acid