title: Low endotoxic activity of lipopolysaccharides isolated from Bradyrhizobium, Mesorhizobium, and Azospirillum strains.
abstract: The endotoxic activities of lipopolysaccharides (LPS) isolated from different strains of rhizobia and rhizobacteria (Bradyrhizobium, Mesorhizobium, and Azospirillum) were compared to those of Salmonella enterica sv. Typhimurium LPS. The biological activity of all the examined preparations, measured as Limulus lysate gelation, production of tumor necrosis factor (TNF), interleukin-1β (IL-1β), and interleukin-6 (IL-6), and nitrogen oxide (NO) induction in human myelomonocytic cells (line THP-1), was considerably lower than that of the reference enterobacterial endotoxin. Among the rhizobial lipopolysaccharides, the activities of Mesorhizobium huakuii and Azospirillum lipoferum LPSs were higher than those of the LPS preparations from five strains of Bradyrhizobium. The weak endotoxic activity of the examined preparations was correlated with differences in lipid A structure compared to Salmonella.
authors.affiliation: Genetics and Microbiology Virology and Immunology, Maria Curie-Sklodowska University, Akademicka 19, 20-033 Lublin, Poland. ikoma@hektor.umcs.lublin.pl
authors.forename: Iwona
authors.initials: I
authors.lastname: Komaniecka
authors.forename: Barbara
authors.initials: B
authors.lastname: Zdzisinska
authors.forename: Martyna
authors.initials: M
authors.lastname: Kandefer-Szerszen
authors.forename: Adam
authors.initials: A
authors.lastname: Choma
mesh_terms: D020561:Alphaproteobacteria / Q000472:pathogenicity*; D016949:Azospirillum / Q000472:pathogenicity*; D020369:Bradyrhizobium / Q000472:pathogenicity*; D002460:Cell Line; D016207:Cytokines / Q000096:biosynthesis; D006801:Humans; D008070:Lipopolysaccharides / Q000302:isolation & purification / Q000633:toxicity*; D009569:Nitric Oxide / Q000096:biosynthesis
chemical_list: D016207:Cytokines; D008070:Lipopolysaccharides; D009569:Nitric Oxide