title: Molecular characterization of the endoplasmic reticulum: insights from proteomic studies.
abstract: The endoplasmic reticulum (ER) is a multifunctional intracellular organelle responsible for the synthesis, processing and trafficking of a wide variety of proteins essential for cell growth and survival. Therefore, comprehensive characterization of the ER proteome is of great importance to the understanding of its functions and has been actively pursued in the past decade by scientists in the proteomics field. This review summarizes major proteomic studies published in the past decade that focused on the ER proteome. We evaluate the data sets obtained from two different organs, liver and pancreas each of which contains a primary cell type (hepatocyte and acinar cell) with specialized functions. We also discuss how the nature of the proteins uncovered is related to the methods of organelle purification, organelle purity and the techniques used for protein separation prior to MS. In addition, this review also puts emphasis on the biological insights gained from these studies regarding the molecular functions of the ER including protein synthesis and translocation, protein folding and quality control, ER-associated degradation and ER stress, ER export and membrane trafficking, calcium homeostasis and detoxification and drug metabolism.
authors.affiliation: Department of Molecular and Integrative Physiology, The University of Michigan, Ann Arbor, MI, USA. xchen@med.wayne.edu
authors.forename: Xuequn
authors.initials: X
authors.lastname: Chen
authors.forename: Alla
authors.initials: A
authors.lastname: Karnovsky
authors.forename: Maria Dolors
authors.initials: MD
authors.lastname: Sans
authors.forename: Philip C
authors.initials: PC
authors.lastname: Andrews
authors.forename: John A
authors.initials: JA
authors.lastname: Williams
mesh_terms: D000818:Animals; D002417:Cattle; D006224:Cricetinae; D030562:Databases, Protein; D004285:Dogs; D004721:Endoplasmic Reticulum / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D006801:Humans; D051379:Mice; D040901:Proteomics / Q000379:methods; D051381:Rats