title: Immunohistochemical detection of arteriolar hyperplasia in canine liver biopsy samples using the claudin-5 antibody.
abstract: Claudins are key tight junctional proteins between adjacent epithelial, mesothelial or endothelial cells, which are responsible for the permeability of the paracellular space. This paper describes that the endothelial cells of normal hepatic arterioles, portal venules and portal lymphatics as well as the endothelium of sinusoids from dogs show strong membranous claudin-5 cross-reactivity. In 25 liver biopsy samples taken from dogs with portal vein hypoperfusion, an increased number of arterioles was detected in the portal areas (PAs) by the use of humanised anti-claudin-5 antibody. The increased number of hyperplastic hepatic arterioles per PA was 5-6, 8-12 and 15-20 in the case of small, medium-sized and large PAs, respectively. It is suggested that the claudin-5 marker can improve the detection of hepatic arteriolar proliferation in the PAs of liver samples.
authors.affiliation: Department of Pathology and Forensic Veterinary Medicine, István u. 2 H-1078 Budapest, Hungary. Jakab.Csaba@aotk.szie.hu
authors.forename: Csaba
authors.initials: C
authors.lastname: Jakab
authors.forename: Miklós
authors.initials: M
authors.lastname: Rusvai
authors.forename: Péter
authors.initials: P
authors.lastname: Gálfi
authors.forename: Agnes
authors.initials: A
authors.lastname: Szabára
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Szabó
authors.forename: Janina
authors.initials: J
authors.lastname: Kulka
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D001160:Arterioles / Q000473:pathology*; D001706:Biopsy / Q000662:veterinary; D062507:Claudin-5; D004283:Dog Diseases / Q000175:diagnosis / Q000473:pathology*; D004285:Dogs; D005786:Gene Expression Regulation / Q000502:physiology; D006965:Hyperplasia / Q000662:veterinary; D007150:Immunohistochemistry / Q000662:veterinary*; D008099:Liver / Q000098:blood supply* / Q000378:metabolism / Q000473:pathology; D008107:Liver Diseases / Q000175:diagnosis / Q000473:pathology / Q000662:veterinary*; D008565:Membrane Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D011169:Portal Vein / Q000473:pathology
chemical_list: D000911:Antibodies, Monoclonal; D062507:Claudin-5; D008565:Membrane Proteins