title: Curcumin induces paraoxonase 1 in cultured hepatocytes in vitro but not in mouse liver in vivo.
abstract: Paraoxonase 1 (PON1) is an enzyme that is mainly synthesised in the liver and protects LDL from oxidation, thereby exhibiting antiatherogenic properties. Using a luciferase reporter gene assay, we tested curcumin for its ability to induce PON1 in Huh7 hepatocytes in culture. Curcumin ( ≥ 10 μmol/l) dose-dependently induced PON1 transactivation in Huh7 cells. However, dietary supplementation of female B6C3F1 mice with curcumin (500 mg/kg diet) for 2 weeks did not increase the hepatic PON1 mRNA and protein levels. No curcumin was detectable in the plasma of the 12 h fasted mice. In conclusion, curcumin may be a potent PON1 inducer in cultured cells in vitro, but not in the liver of curcumin-fed mice because of its low concentrations in vivo.
authors.affiliation: Institute of Human Nutrition and Food Science, Christian-Albrechts-University of Kiel, Hermann-Rodewald-Street 6, 24118 Kiel, Germany.
authors.forename: Charlotte
authors.initials: C
authors.lastname: Schrader
authors.forename: Christina
authors.initials: C
authors.lastname: Schiborr
authors.forename: Jan
authors.initials: J
authors.lastname: Frank
authors.forename: Gerald
authors.initials: G
authors.lastname: Rimbach
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D043303:Aryldialkylphosphatase / Q000096:biosynthesis* / Q000235:genetics; D050197:Atherosclerosis / Q000517:prevention & control; D001483:Base Sequence; D002460:Cell Line; D003474:Curcumin / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000494:pharmacology*; D017931:DNA Primers / Q000235:genetics; D019587:Dietary Supplements; D004790:Enzyme Induction / Q000187:drug effects; D005260:Female; D022781:Hepatocytes / Q000187:drug effects* / Q000201:enzymology*; D006801:Humans; D066298:In Vitro Techniques; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D051379:Mice; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015533:Transcriptional Activation / Q000187:drug effects; D014162:Transfection
chemical_list: D000975:Antioxidants; D017931:DNA Primers; D012333:RNA, Messenger; D043303:Aryldialkylphosphatase; C468406:PON1 protein, human; D003474:Curcumin