title: Prevalence of porcine circoviruses in Transylvanian wild boars, detected by real-time PCR--short communication.
abstract: Porcine circoviruses (PCV) are widespread in domestic pigs worldwide and there is growing information about the presence of PCV in other suid species. Based on serological studies with sera of wild boars, it was established that PCV1 was present in these animals and antibodies specific to PCV2 were also detected in wild boars living in captivity or in sylvatic areas, both with or without clinical signs of PMWS. Studies including PCV2 genome or antigen detection confirmed the previous findings. This is the first report about the presence of PCV in Transylvanian wild boar populations. Four hundred and sixty-nine samples were collected and grouped according to geographic origin, tested for the presence of PCV DNA using a real-time quantitative polymerase chain reaction assay, and 13.52% of the animals proved to be positive for one or in three cases both of the PCV genotypes. PCV2 was detected in all of the PCV-positive samples.
authors.affiliation: Department of Infectious Diseases, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, 400372, Manastur 3-5, Romania.
authors.forename: Daniel
authors.initials: D
authors.lastname: Cadar
authors.forename: Attila
authors.initials: A
authors.lastname: Cságola
authors.forename: Marina
authors.initials: M
authors.lastname: Spinu
authors.forename: Adám
authors.initials: A
authors.lastname: Dán
authors.forename: Krisztina
authors.initials: K
authors.lastname: Ursu
authors.forename: Márta
authors.initials: M
authors.lastname: Lorincz
authors.forename: Tamás
authors.initials: T
authors.lastname: Tuboly
mesh_terms: D000818:Animals; D018173:Circoviridae Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D017925:Circovirus / Q000302:isolation & purification*; D006814:Hungary / Q000453:epidemiology; D016133:Polymerase Chain Reaction / Q000662:veterinary*; D015995:Prevalence; D012680:Sensitivity and Specificity; D034421:Sus scrofa