title: Urinary hepatocyte growth factor indicates ischemia/reperfusion injury after kidney transplantation.
abstract: BACKGROUND
  Despite the development of immunosuppressive regimens in kidney transplantation, long-term graft survival rates have not increased significantly. One of the causes of long-term graft loss is ischemia-reperfusion insult. Hepatocyte growth factor (HGF) is a regenerative factor produced in response to injury.
  
  
  OBJECTIVE
  Our aim was to assess the effect of HGF and xanthine oxidase (indicators of ischemia/reperfusion insult) on early and late kidney function.
  
  
  METHODS
  In 17 patients, HGF levels in urine and xanthine oxidase activity in blood were examined 1, 7, 14, 30 days, 3 and 6 months after kidney transplantation. We also measured 24-hour diuresis and serum creatinine levels after transplantation.
  
  
  RESULTS
  Urinary HGF levels were highest 1 day after transplantation. During the following week, it rapidly decreased and was maintained at similar levels in the later period. Creatinine at 1 day showed a positive correlation with urinary HGF levels at 1 day and at 3 months (R = 0.54, P <0.05 and R = 0.82, P <0.01, respectively). Creatinine at 7 days positively correlated with HGF levels at 6 months (R = 0.82, P <0.05). HGF levels at 1 day and at 6 months positively correlated with xanthine oxidase activity at 1 day (R = 0.73, P <0.001 and R = 0.77, P <0.02, respectively). A negative correlation was observed between HGF levels at 6 months and diuresis 1 and 7 days after transplantation (R = -0.99, P <0.00 001 and R = -0.77, P <0.05, respectively).
  
  
  CONCLUSIONS
  Urinary HGF is a good marker of perioperative kidney damage and may affect long-term graft function.
authors.affiliation: Department of Nephrology, Transplantology and Internal Medicine, Pomeranian Medical University, Szczecin, Poland. ewakwiat@gmail.com
authors.forename: Ewa
authors.initials: E
authors.lastname: Kwiatkowska
authors.forename: Karolina
authors.initials: K
authors.lastname: Kędzierska
authors.forename: Joanna
authors.initials: J
authors.lastname: Bober
authors.forename: Barbara
authors.initials: B
authors.lastname: Dołęgowska
authors.forename: Violetta
authors.initials: V
authors.lastname: Dziedziejko
authors.forename: Edyta
authors.initials: E
authors.lastname: Gołembiewska
authors.forename: Kazimierz
authors.initials: K
authors.lastname: Ciechanowski
authors.forename: Magdalena
authors.initials: M
authors.lastname: Wiśniewska
mesh_terms: D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000097:blood / Q000652:urine; D005260:Female; D017228:Hepatocyte Growth Factor / Q000652:urine*; D006801:Humans; D016030:Kidney Transplantation / Q000009:adverse effects*; D008297:Male; D008875:Middle Aged; D011044:Poland; D011184:Postoperative Period; D015427:Reperfusion Injury / Q000097:blood / Q000175:diagnosis* / Q000209:etiology* / Q000652:urine; D014969:Xanthine Oxidase / Q000097:blood*; D055815:Young Adult
chemical_list: D015415:Biomarkers; D017228:Hepatocyte Growth Factor; D014969:Xanthine Oxidase