title: Immunolocalization of ghrelin in the stomach of Sprague-Dawley rat.
abstract: To investigate the distribution of ghrelin in different regions of stomach in Sprague-Dawley rat, and attempt to compare with those of humans and other mammalian species in this study, the stomach of rats was divided into five sections, cardia, fundus, greater curvature, lesser curvature and pylorus. Immunohistochemistry and Western blotting were performed to investigate the ghrelin-producing cells. The immunolocalization and protein levels of ghrelin differed significantly in different regions of stomach in rats. It was present at a high level in the greater curvature of the pars glandularis, and the lesser curvature. In the fundus and pylorus, no ghrelin immunoreactive cells were detected. In this study, we elucidated the distribution of ghrelin-producing cells in different regions of rat stomach in detail for the first time. It is further considered that the differences of ghrelin distribution in stomach of different species may induce different stimulatory effects on fat accumulation and metabolism.
authors.affiliation: Department of Anatomy, Tokyo Medical University, Tokyo, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Li
authors.forename: S-Q
authors.initials: SQ
authors.lastname: Yi
authors.forename: H-X
authors.initials: HX
authors.lastname: Wang
authors.forename: N
authors.initials: N
authors.lastname: Yi
authors.forename: Y
authors.initials: Y
authors.lastname: Ogawa
authors.forename: N
authors.initials: N
authors.lastname: Ozaki
authors.forename: M
authors.initials: M
authors.lastname: Itoh
mesh_terms: D000818:Animals; D015153:Blotting, Western; D005748:Gastric Fundus / Q000378:metabolism; D005753:Gastric Mucosa / Q000378:metabolism; D054439:Ghrelin / Q000032:analysis* / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D036361:Peptide Hormones / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013270:Stomach / Q000737:chemistry*
chemical_list: D054439:Ghrelin; D036361:Peptide Hormones