title: Development of an ultra-performance liquid chromatography-tandem mass spectrometry micromethod for quantification of lamotrigine in human plasma and its use in a bioequivalence trial.
abstract: BACKGROUND
  The aim of the present work was to develop a chromatographic technique coupled with mass spectrometry for the measurement of lamotrigine in plasma. Lamotrigine and guanabenz (internal standard) were measured by selected reaction monitoring. The method was validated and applied in a bioequivalence trial on 26 female volunteers. Lamotrigine chewable tablets (100 mg) were administered and monitored for up to 96 h.
  
  
  RESULTS
  The method was linear between 0.05 and 5.0 µg/ml, with acceptable stability, accuracy and precision. Mean maximum plasma concentration was 1.37 µg/ml and was reached at 1.6 h postdose. Elimination half-life was 32.7 h.
  
  
  CONCLUSIONS
  Lamotrigine tablets were bioequivalent. Ultra-performance liquid chromatography with tandem mass spectrometry represents a powerful tool in terms of sensitivity, specificity and high-throughput analysis.
authors.affiliation: Servicio de Investigación de Farmacología Clínica (405-E), Hospital General de México. Dr Balmis No. 148, Col. Doctores, CP 06726 México D.F., México. gabmarcelin@hotmail.com
authors.forename: Gabriel
authors.initials: G
authors.lastname: Marcelín-Jiménez
authors.forename: Alionka Citlali
authors.initials: AC
authors.lastname: P Angeles Moreno
authors.forename: Luis
authors.initials: L
authors.lastname: Mendoza-Morales
authors.forename: Liliana
authors.initials: L
authors.lastname: Rivera-Espinosa
authors.forename: Miriam Morales
authors.initials: MM
authors.lastname: Martínez
mesh_terms: D000284:Administration, Oral; D000328:Adult; D000927:Anticonvulsants / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics* / Q000494:pharmacology; D000959:Antihypertensive Agents / Q000032:analysis; D002853:Chromatography, Liquid; D005260:Female; D006143:Guanabenz / Q000032:analysis; D006207:Half-Life; D057166:High-Throughput Screening Assays; D006801:Humans; D000077213:Lamotrigine; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry; D013810:Therapeutic Equivalency; D014227:Triazines / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics* / Q000494:pharmacology; D055815:Young Adult
chemical_list: D000927:Anticonvulsants; D000959:Antihypertensive Agents; D014227:Triazines; D006143:Guanabenz; D000077213:Lamotrigine