title: DNA cleavage system of nanosized graphene oxide sheets and copper ions.
abstract: The exploration of efficient DNA intercalative agents (intercalators) is essential for understanding DNA scission, repair, and signal transduction. In this work, we explored systematically the graphene oxide (GO) interaction with DNA molecules using fluorescence spectroscopic (FL) and circular dichroism (CD) studies, gel electrophoresis, and DNA thermal denaturation. We demonstrated that the GO nanosheets could intercalate efficiently into DNA molecules. Significantly, we illustrated that the scission of DNA by GO sheets combining with copper ions could take place pronouncedly. The scission of DNA by the GO/Cu(2+) system is critically dependent on the concentrations of GO and Cu(2+) and their ratio. DNA cleavage ability exhibited by the GO with several other metal ions and the fact that GO/Cu(2+)-cleaved DNA fragments can be partially relegated suggest that the mechanism of DNA cleavage by the GO/metal ion system is oxidative and hydrolytic. The result reveals that the GO/Cu(2+) could be used as a DNA cleaving system that should find many practical applications in biotechnology and as therapeutic agents.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, People's Republic of China.
authors.forename: Hongliu
authors.initials: H
authors.lastname: Ren
authors.forename: Chong
authors.initials: C
authors.lastname: Wang
authors.forename: Jiali
authors.initials: J
authors.lastname: Zhang
authors.forename: Xuejiao
authors.initials: X
authors.lastname: Zhou
authors.forename: Dafeng
authors.initials: D
authors.lastname: Xu
authors.forename: Jing
authors.initials: J
authors.lastname: Zheng
authors.forename: Shouwu
authors.initials: S
authors.lastname: Guo
authors.forename: Jingyan
authors.initials: J
authors.lastname: Zhang
mesh_terms: D002942:Circular Dichroism; D003300:Copper / Q000737:chemistry* / Q000378:metabolism* / Q000494:pharmacology; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D006108:Graphite / Q000737:chemistry* / Q000378:metabolism* / Q000494:pharmacology; D007364:Intercalating Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D008958:Models, Molecular; D049329:Nanostructures / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D010087:Oxides / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D007364:Intercalating Agents; D010087:Oxides; D006108:Graphite; D003300:Copper; D004247:DNA