title: Dental pulp stem cell migration.
abstract: BACKGROUND
  The purpose of this in vitro study was to investigate the migration of dental pulp stem cells (DPSCs) in response to chemotactants and extracellular matrix proteins (EMPs). This DPSC signaling information is needed to help understand tooth regeneration after injury and to develop some future regenerative endodontic therapies.
  
  
  METHODS
  DPSCs were released by trypsinization and plated on transwell filters. The chemotactants were recombinant sphingosine-1-phosphate (S1P), fibroblast growth factor (FGF), epidermal growth factor (EGF), or transforming growth factor beta-1 (TGF-β1), and the EMPs were collagen-1, collagen-IV, laminin, and fibronectin. Data were analyzed by using analysis of variance (ANOVA) statistical tests for cell migration.
  
  
  RESULTS
  S1P induced more vigorous DPSC migration in comparison with the other TGF- β1, FGF, or EFG chemotactants (ANOVA, P < .05). Laminin induced more vigorous DPSC migration in comparison with the other EMPs (ANOVA, P < .05).
  
  
  CONCLUSIONS
  The EMPs, particularly laminin, and chemotactants, particularly S1P and TGF-β1, were found to be important promoters of DPSC migration. The interplay between the EMPs, blood lipid, serum, and chemotactants suggests that the migration of DPSC is highly regulated. Specific chemotactants and EMPs might mediate the process of pulp-dentin regeneration after tooth injury, and they could be used as part of regenerative endodontic therapy.
authors.affiliation: College of Dental Medicine, Nova Southeastern University, Fort Lauderdale, Florida 33328-2018, USA.
authors.forename: Cameron
authors.initials: C
authors.lastname: Howard
authors.forename: Peter E
authors.initials: PE
authors.lastname: Murray
authors.forename: Kenneth N
authors.initials: KN
authors.lastname: Namerow
mesh_terms: D053687:Adult Stem Cells / Q000187:drug effects* / Q000502:physiology; D000704:Analysis of Variance; D018929:Cell Culture Techniques; D002460:Cell Line; D002630:Chemotactic Factors / Q000494:pharmacology*; D002633:Chemotaxis / Q000187:drug effects* / Q000502:physiology; D003782:Dental Pulp / Q000166:cytology* / Q000187:drug effects; D042783:Endothelial Cells / Q000187:drug effects; D016326:Extracellular Matrix Proteins / Q000494:pharmacology*; D006801:Humans; D007797:Laminin / Q000494:pharmacology; D008246:Lysophospholipids / Q000494:pharmacology; D032389:Myocytes, Smooth Muscle / Q000187:drug effects; D011994:Recombinant Proteins / Q000494:pharmacology; D012038:Regeneration / Q000187:drug effects; D015398:Signal Transduction / Q000187:drug effects; D013110:Sphingosine / Q000031:analogs & derivatives / Q000494:pharmacology; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology
chemical_list: D002630:Chemotactic Factors; D016326:Extracellular Matrix Proteins; D007797:Laminin; D008246:Lysophospholipids; D011994:Recombinant Proteins; D053773:Transforming Growth Factor beta1; C060506:sphingosine 1-phosphate; D013110:Sphingosine