title: Antifibrotic activity of coumarins from Cnidium monnieri fruits in HSC-T6 hepatic stellate cells.
abstract: The CHCl(3) fraction of Cnidium monnieri fruits significantly inhibited the proliferation of hepatic stellate cells in an in-vitro assay system employing HSC-T6 hepatic stellate cell lines. Activity-guided fractionation of the CHCl(3) fraction of C. monnieri led to the isolation of ten coumarins: osthol (1), meranzin (2), auraptenol (3), meranzin hydrate (4), 7-hydroxy-8-methoxy coumarin (5), imperatorin (6), xanthotoxol (7), xanthotoxin (8), bergapten (9) and isopimpinellin (10). Of these, compounds 1 and 6 significantly inhibited proliferation of HSCs in a time- and concentration-dependent manner. In addition, compounds 1 and 6 significantly reduced collagen content in HSC-T6 cells.
authors.affiliation: College of Pharmacy, Chungbuk National University, 410 Seongbong-ro, Heungduk-gu, Cheongju 361-763, Korea.
authors.forename: Eunjin
authors.initials: E
authors.lastname: Shin
authors.forename: Chul
authors.initials: C
authors.lastname: Lee
authors.forename: Sang Hyun
authors.initials: SH
authors.lastname: Sung
authors.forename: Young Choong
authors.initials: YC
authors.lastname: Kim
authors.forename: Bang Yeon
authors.initials: BY
authors.lastname: Hwang
authors.forename: Mi Kyeong
authors.initials: MK
authors.lastname: Lee
mesh_terms: D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D031048:Cnidium / Q000737:chemistry*; D003374:Coumarins / Q000737:chemistry* / Q000494:pharmacology*; D005638:Fruit / Q000737:chemistry*; D055166:Hepatic Stellate Cells / Q000166:cytology / Q000187:drug effects*; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats
chemical_list: D003374:Coumarins; D010936:Plant Extracts