title: Screening of a minimal enriched P450 BM3 mutant library for hydroxylation of cyclic and acyclic alkanes.
abstract: A minimal enriched P450 BM3 library was screened for the ability to oxidize inert cyclic and acyclic alkanes. The F87A/A328V mutant was found to effectively hydroxylate cyclooctane, cyclodecane and cyclododecane. F87V/A328F with high activity towards cyclooctane hydroxylated acyclic n-octane to 2-(R)-octanol (46% ee) with high regioselectivity (92%).
authors.affiliation: Institute of Technical Biochemistry, Universitaet of Stuttgart, Allmandring 31, 70569 Stuttgart, Germany.
authors.forename: Evelyne
authors.initials: E
authors.lastname: Weber
authors.forename: Alexander
authors.initials: A
authors.lastname: Seifert
authors.forename: Mihaela
authors.initials: M
authors.lastname: Antonovici
authors.forename: Christopher
authors.initials: C
authors.lastname: Geinitz
authors.forename: Jürgen
authors.initials: J
authors.lastname: Pleiss
authors.forename: Vlada B
authors.initials: VB
authors.lastname: Urlacher
mesh_terms: D000473:Alkanes / Q000737:chemistry / Q000378:metabolism*; D001410:Bacillus megaterium / Q000201:enzymology*; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D003500:Cyclization; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics* / Q000378:metabolism*; D006900:Hydroxylation; D008958:Models, Molecular; D009154:Mutation; D009251:NADPH-Ferrihemoprotein Reductase / Q000235:genetics* / Q000378:metabolism*; D010084:Oxidation-Reduction; D013379:Substrate Specificity
chemical_list: D000473:Alkanes; D001426:Bacterial Proteins; D003577:Cytochrome P-450 Enzyme System; D009251:NADPH-Ferrihemoprotein Reductase; C052423:flavocytochrome P450 BM3 monoxygenases