title: Application of dosimetry systems and cytogenetic status of the child population exposed to diagnostic X-rays by use of the cytokinesis-block micronucleus cytome assay.
abstract: Low-dose ionizing radiation used for medical purposes is one of the definite risk factors for cancer development, and children exposed to ionizing radiation are at a relatively greater cancer risk as they have more rapidly dividing cells than adults and have longer life expectancy. Since cytokinesis-block micronucleus cytome (CBMN Cyt) assay has become one of the standard endpoints for radiation biological dosimetry, we used that assay in the present work for the assessment of different types of chromosomal damage in children exposed to diagnostic X-ray procedures. Twenty children all with pulmonary diseases between the ages of 4 and 14 years (11.30 ± 2.74) were evaluated. Absorbed dose measurements were conducted for posterior-anterior projection on the forehead, thyroid gland, gonads, chest and back. Doses were measured using thermoluminescence and radiophotoluminescent dosimetry systems. It was shown that, after diagnostic X-rays, the mean total number of CBMN Cyt assay parameters (micronucleus, nucleoplasmic bridges and nuclear buds) was significantly higher than prior to diagnostic procedure and that interindividual differences existed for each monitored child. For the nuclear division index counted prior and after examination, no significant differences were noted among mean group values. These data suggest that even low-dose diagnostic X-ray exposure may induce damaging effect in the somatic DNA of exposed children, indicating that immense care should be given in both minimizing and optimizing radiation exposure to diminish the radiation burden, especially in the youngest population.
authors.affiliation: Institute for Medical Research and Occupational Health, Mutagenesis Unit, 10000 Zagreb, Croatia.
authors.forename: Goran
authors.initials: G
authors.lastname: Gajski
authors.forename: Durđica
authors.initials: D
authors.lastname: Milković
authors.forename: Mária
authors.initials: M
authors.lastname: Ranogajec-Komor
authors.forename: Saveta
authors.initials: S
authors.lastname: Miljanić
authors.forename: Vera
authors.initials: V
authors.lastname: Garaj-Vrhovac
mesh_terms: D000293:Adolescent; D002467:Cell Nucleus / Q000528:radiation effects; D002648:Child; D002675:Child, Preschool; D020732:Cytogenetic Analysis; D048749:Cytokinesis / Q000528:radiation effects*; D004249:DNA Damage / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D005260:Female; D006801:Humans; D008297:Male; D015162:Micronucleus Tests / Q000379:methods*; D011874:Radiometry / Q000379:methods*; D014965:X-Rays / Q000009:adverse effects*