title: Plasma polymer and PEG-based coatings for DNA, protein and cell microarrays.
abstract: DNA, protein and cell microarrays are increasingly used in a multitude of bioassays. All of these arrays require substrates that are suitable for the immobilisation and display of arrayed probe molecules whilst at the same time resisting non-specific interactions of biomolecules with the substrate in areas between printed spots. To meet these conflicting requirements, three different approaches have been developed, all of which were based on low-fouling, high-density poly(ethylene glycol) (PEG) background coatings. In the first approach, the coating was based on allylamine plasma polymerisation (ALAPP) and the subsequent high-density grafting of PEG, followed by the generation of a surface chemical pattern using laser ablation. In the second approach, a photoreactive polymer was printed on the same ALAPP-PEG background. The third approach was based on ALAPP deposition followed by the formation of a multifunctional layer by spin coating a PEG-based polymer that also displayed epoxy groups. The successful demonstration of DNA, protein and cell microarrays has been achieved on each of these coatings.
authors.affiliation: Laboratory of Biophysics and Surface Analysis, University of Nottingham, Nottingham, UK. andrew.hook@nottingham.ac.uk
authors.forename: Andrew L
authors.initials: AL
authors.lastname: Hook
authors.forename: Nicolas H
authors.initials: NH
authors.lastname: Voelcker
authors.forename: Helmut
authors.initials: H
authors.lastname: Thissen
mesh_terms: D000327:Adsorption; D000499:Allylamine / Q000737:chemistry; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D005740:Gases; D011092:Polyethylene Glycols / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D013379:Substrate Specificity; D013499:Surface Properties; D046888:Tissue Array Analysis / Q000379:methods*
chemical_list: D020099:Coated Materials, Biocompatible; D005740:Gases; D011108:Polymers; D011506:Proteins; D011092:Polyethylene Glycols; D000499:Allylamine