title: Adipose-derived stem cells for skin regeneration.
abstract: Intractable skin ulcers resulting from diabetes, ischemia and collagen diseases represent significant problems with few solutions. Cell-based therapy may hold promise in overcoming such disorders. In order to establish a suitable experimental model for the treatment of such ulcers using stem cells, this chapter describes detailed methods for: (1) isolation of stem cells from adipose tissue, termed adipose-derived stem cells (ASCs), (2) preparing a hybrid-type artificial dermis that consists of a type I collagen sponge and ASCs, (3) preparing intractable ulcers using Mitomycin C, and (4) evaluating the effect of wound healing histologically. ASCs seeded onto a type I collagen sponge are applied to intractable ulcers induced by topical application of Mitomycin C. Histological evaluation after 1 and 2 weeks revealed an increase in capillary density and granulation thickness of the hybrid-type artificial dermis. These findings suggest that ASCs may have a positive effect on wound healing and may be a useful tool for future cell-based therapy.
authors.affiliation: Department of Plastic and Reconstructive Surgery, School of Medicine, Juntendo University, Tokyo, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Mizuno
authors.forename: Masaki
authors.initials: M
authors.lastname: Nambu
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D002469:Cell Separation; D002478:Cells, Cultured; D008297:Male; D051381:Rats; D011916:Rats, Inbred F344; D012038:Regeneration / Q000502:physiology*; D044968:Regenerative Medicine / Q000379:methods*; D012867:Skin / Q000378:metabolism*; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology*; D014456:Ulcer / Q000473:pathology / Q000628:therapy