title: [Asymmetric dimethylarginine (ADMA) in children with diabetes type 1].
abstract: BACKGROUND
  Asymmetric dimethylarginine (ADMA), a naturally occurring product of asymmetric methylation of proteins, is an endogenous inhibitor of endothelial nitric oxide synthase. ADMA is now recognized as an independent marker of endothelial dysfunction and atherosclerosis. Data concerning ADMA level in type 1 diabetes (DM1) are controversial. The aim of the study was to evaluate ADMA level in children with DM1, without clinical evidence of vascular complications, with particular attention to additional cardiovascular risk factors (hypertension, obesity, hyperlipidemia).
  
  
  METHODS
  The study group included 72 children with DM1, aged mean 15±3 yrs (8-20 yrs), 33 boys and 39 girls, with diabetes duration time mean 6.6±3.5 yrs (1-14 yrs), HBA1c mean level 8.2±2.3% (5.6-15%). The control group consisted of 41 (19 boys and 22 girls) healthy children, aged mean--14.8±2.6 yrs, from 8 to 18 yrs, gender matched, with no family history of cardiovascular disease. ADMA level was determined in plasma using ELISA kit (DLD Diagnostica, Hamburg, Germany)
  
  
  RESULTS
  ADMA level was similar in children with diabetes and in the control group: 0.69±0.33 vs. 0.7±0.27 μmol/L, ns. We did not find differences in ADMA level in diabetic children with the presence of additional diseases being cardiovascular risk factors. In the group of 13 children with hypertension ADMA level was the highest: 0.79±0.25 μmol/L, but the difference was statistically insignificant in comparison to children with diabetes without hypertension and in comparison to healthy controls.
  
  
  CONCLUSIONS
  Children with DM1, without clinically evident vascular complications, have ADMA levels similar to healthy children. A possible relationship between ADMA and hypertension in these patients requires further investigation.
authors.affiliation: Klinika Pediatrii, Endokrynologii, Diabetologii z Pododdzialem Kardiologii Uniwersytetu Medycznego w Bialymstoku. bglowinska@poczta.onet.pl
authors.forename: Barbara
authors.initials: B
authors.lastname: Głowińska-Olszewska
authors.forename: Włodzimierz
authors.initials: W
authors.lastname: Luczyński
authors.forename: Jolanta
authors.initials: J
authors.lastname: Jabłońska
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Otocka
authors.forename: Bożena
authors.initials: B
authors.lastname: Florys
authors.forename: Artur
authors.initials: A
authors.lastname: Bossowski
mesh_terms: D000293:Adolescent; D000328:Adult; D001120:Arginine / Q000031:analogs & derivatives* / Q000097:blood; D015415:Biomarkers / Q000378:metabolism; D002648:Child; D015897:Comorbidity; D003922:Diabetes Mellitus, Type 1 / Q000097:blood* / Q000453:epidemiology; D004730:Endothelium, Vascular / Q000201:enzymology; D005260:Female; D006801:Humans; D006973:Hypertension / Q000097:blood / Q000453:epidemiology; D008297:Male; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors; D055815:Young Adult
chemical_list: D015415:Biomarkers; C018524:N,N-dimethylarginine; D001120:Arginine; D019001:Nitric Oxide Synthase