title: The outer chloroplast envelope protein OEP16-1 for plastid import of NADPH:protochlorophyllide oxidoreductase A in Arabidopsis thaliana.
abstract: The outer plastid envelope protein OEP16-1 was previously identified as an amino acid-selective channel protein and translocation pore for NADPH:protochlorophyllide oxidoreductase A (PORA). Reverse genetic approaches used to dissect these mutually not exclusive functions of OEP16-1 in planta have led to descriptions of different phenotypes resulting from the presence of several mutant lines in the SALK_024018 seed stock. In addition to the T-DNA insertion in the AtOEP16-1 gene, lines were purified that contain two additional T-DNA insertions and as yet unidentified point mutations. In a first attempt to resolve the genetic basis of four different lines in the SALK_024018 seed stock, we used genetic transformation with the OEP16-1 cDNA and segregation analyses after crossing out presumed point mutations. We show that AtOEP16-1 is involved in PORA precursor import and by virtue of this activity confers photoprotection onto etiolated seedlings during greening.
authors.affiliation: Université Joseph Fourier, F-38041 Grenoble cedex 9, France.
authors.forename: Iga
authors.initials: I
authors.lastname: Samol
authors.forename: Claudia
authors.initials: C
authors.lastname: Rossig
authors.forename: Frank
authors.initials: F
authors.lastname: Buhr
authors.forename: Armin
authors.initials: A
authors.lastname: Springer
authors.forename: Stephan
authors.initials: S
authors.lastname: Pollmann
authors.forename: Abder
authors.initials: A
authors.lastname: Lahroussi
authors.forename: Diter
authors.initials: D
authors.lastname: von Wettstein
authors.forename: Christiane
authors.initials: C
authors.lastname: Reinbothe
authors.forename: Steffen
authors.initials: S
authors.lastname: Reinbothe
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D005816:Genetic Complementation Test; D007473:Ion Channels / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D018087:Plastids / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D007473:Ion Channels; C512221:OEP16-L protein, Arabidopsis