title: Endogenous ADP-ribosylation of elongation factor-2 by interleukin-1β.
abstract: Eukaryotic elongation factor-2 (eEF-2) catalyses the motion of the growing peptide chain relative to the mRNA at the ribosomes during protein synthesis. This highly conserved G-protein is the specific target of two lethal bacterial toxins, Pseudomonas aeruginosa exotoxin A and diphtheria toxin. These toxins exert their detrimental action by ADP-ribosylating a biologically unique posttranslationally modified histidine residue (diphthamide(715)) within eEF-2, thus inactivating the enzyme. Diphthamide(715) is also the target of endogenous (mono) ADP-ribosyl transferase activity. In this article, we report the first known activator of endogenous ADP-ribosylation of eEF-2, interleukin-1β (IL-1β). Thereby, systemic inflammatory processes may link to protein synthesis regulation.
authors.affiliation: Department of Medicine III, University Halle-Wittenberg, Halle, Germany.
authors.forename: Doris
authors.initials: D
authors.lastname: Jäger
authors.forename: Karl
authors.initials: K
authors.lastname: Werdan
authors.forename: Ursula
authors.initials: U
authors.lastname: Müller-Werdan
mesh_terms: D036002:ADP Ribose Transferases / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D001427:Bacterial Toxins / Q000378:metabolism; D002478:Cells, Cultured; D005098:Exotoxins / Q000378:metabolism; D006639:Histidine / Q000031:analogs & derivatives / Q000378:metabolism; D006801:Humans; D053583:Interleukin-1beta / Q000378:metabolism*; D032383:Myocytes, Cardiac / Q000276:immunology / Q000378:metabolism*; D020652:Peptide Elongation Factor 2 / Q000378:metabolism*; D014176:Protein Biosynthesis; D011499:Protein Processing, Post-Translational; D051381:Rats; D011994:Recombinant Proteins / Q000378:metabolism; D037521:Virulence Factors / Q000378:metabolism; D000097668:Pseudomonas aeruginosa Exotoxin A
chemical_list: D036002:ADP Ribose Transferases; D001427:Bacterial Toxins; D005098:Exotoxins; D006639:Histidine; D053583:Interleukin-1beta; D020652:Peptide Elongation Factor 2; D011994:Recombinant Proteins; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A; C027527:diphthamide