title: Aptamer-based turn-on fluorescent four-branched quaternary ammonium pyrazine probe for selective thrombin detection.
abstract: In this thrombin detection system, the bright fluorescence of TASPI is almost eliminated by the DNA aptamer TBA (turn-off); however, in the presence of thrombin, it specifically binds to TBA by folding unrestricted TBA into an anti-parallel G-quadruplex structure and then releasing TASPI molecules, resulting in vivid and facile fluorescence recovery (turn-on).
authors.affiliation: College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Hubei, Wuhan, 430072, P. R. China.
authors.forename: Shengyong
authors.initials: S
authors.lastname: Yan
authors.forename: Rong
authors.initials: R
authors.lastname: Huang
authors.forename: Yangyang
authors.initials: Y
authors.lastname: Zhou
authors.forename: Ming
authors.initials: M
authors.lastname: Zhang
authors.forename: Minggang
authors.initials: M
authors.lastname: Deng
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Wang
authors.forename: Xiaocheng
authors.initials: X
authors.lastname: Weng
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhou
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D015335:Molecular Probes / Q000737:chemistry*; D011719:Pyrazines / Q000737:chemistry*; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013917:Thrombin / Q000032:analysis*
chemical_list: D052157:Aptamers, Nucleotide; D005456:Fluorescent Dyes; D015335:Molecular Probes; D011719:Pyrazines; D000644:Quaternary Ammonium Compounds; D013917:Thrombin