title: Tertiary dentin formation after direct pulp capping with odontogenic ameloblast-associated protein in rat teeth.
abstract: BACKGROUND
  Odontogenic ameloblast-associated protein (ODAM) has been shown to be specifically expressed in ameloblasts and odontoblasts and has been suggested to play a role in the mineralization of the enamel, possibly through the regulation of matrix metalloproteinase 20. However, its function in dentin is not clear. The purpose of this study was to evaluate the effect of ODAM on tertiary dentin formation.
  
  
  METHODS
  MDPC-23 odontoblastic cells were cultured, and the effect of recombinant ODAM (rODAM) on mineralized nodule formation was evaluated. Pinpoint pulp exposures were made in rat teeth and then capped with rODAM mixed with a carrier (rODAM group), carrier only (Carrier group), or white mineral trioxide aggregate (WMTA group). After 1, 2, and 4 weeks, odontoblasts and tertiary dentin were investigated histologically and immunohistochemically.
  
  
  RESULTS
  Nodule formation in MDPC-23 cells was enhanced by rODAM treatment. Odontoblasts were polarized and showed a palisade arrangement in the remaining pulp from the rODAM group, but not the Carrier or WMTA groups. In the WMTA group, extensive tertiary dentin along the entire pulp-dentin border obliterated the pulp chamber. In contrast, in the rODAM group, limited reaction of odontoblasts resulted in normal pulp tissue appearance without excessive tertiary dentin formation and obliteration of the pulp cavity. In the Carrier and WMTA groups, bone sialoprotein was immunostained in most of the tertiary dentin, whereas in the rODAM group, dentin sialoprotein expression was immunostained primarily in newly formed reactionary dentin.
  
  
  CONCLUSIONS
  These results suggest that rODAM accelerates reactionary dentin formation close to the pulp exposure area, thereby preserving normal odontoblasts in the remaining pulp.
authors.affiliation: Department of Conservative Dentistry, Dental Research Institute, School of Dentistry, Seoul National University, Seoul, Korea.
authors.forename: In-Seok
authors.initials: IS
authors.lastname: Yang
authors.forename: Dong-Seol
authors.initials: DS
authors.lastname: Lee
authors.forename: Jong-Tae
authors.initials: JT
authors.lastname: Park
authors.forename: Heung-Joong
authors.initials: HJ
authors.lastname: Kim
authors.forename: Ho-Hyun
authors.initials: HH
authors.lastname: Son
authors.forename: Joo-Cheol
authors.initials: JC
authors.lastname: Park
mesh_terms: D016475:3T3 Cells; D017607:Aluminum Compounds / Q000494:pharmacology; D000818:Animals; D017610:Calcium Compounds / Q000494:pharmacology; D002135:Calcium-Binding Proteins / Q000494:pharmacology* / Q000502:physiology*; D002352:Carrier Proteins / Q000494:pharmacology* / Q000502:physiology*; D002460:Cell Line; D003782:Dental Pulp / Q000187:drug effects* / Q000378:metabolism; D003785:Dental Pulp Capping / Q000379:methods*; D003809:Dentin, Secondary / Q000378:metabolism*; D004338:Drug Combinations; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis; D005347:Fibroblasts / Q000187:drug effects; D058574:Integrin-Binding Sialoprotein / Q000096:biosynthesis; D008297:Male; D053519:Matrix Metalloproteinase 20 / Q000378:metabolism; D051379:Mice; D009804:Odontoblasts / Q000187:drug effects*; D010087:Oxides / Q000494:pharmacology; D010750:Phosphoproteins / Q000096:biosynthesis; D057872:Pulp Capping and Pulpectomy Agents / Q000494:pharmacology*; D051381:Rats; D011994:Recombinant Proteins / Q000494:pharmacology; D012795:Sialoglycoproteins / Q000096:biosynthesis; D017640:Silicates / Q000494:pharmacology; D014074:Tooth Calcification / Q000187:drug effects; D000099311:Dentin Sialophosphoprotein
chemical_list: D017607:Aluminum Compounds; D017610:Calcium Compounds; D002135:Calcium-Binding Proteins; D002352:Carrier Proteins; D004338:Drug Combinations; D016326:Extracellular Matrix Proteins; D058574:Integrin-Binding Sialoprotein; D053519:Matrix Metalloproteinase 20; D010087:Oxides; D010750:Phosphoproteins; D057872:Pulp Capping and Pulpectomy Agents; D011994:Recombinant Proteins; D012795:Sialoglycoproteins; D017640:Silicates; D000099311:Dentin Sialophosphoprotein; C556789:ODAM protein, rat; C086631:mineral trioxide aggregate