title: Association analysis about HLA-DRB1, -DQB1 polymorphism and auto-antibodies against α(1)-adrenergic receptors in Chinese patients with essential hypertension.
abstract: The auto-antibodies against α(1)-adrenergic receptors (α(1)-AAs) with agonist activity likes norepinephrine have been discovered in patients with essential hypertension but the mechanism of α(1)-AA production remains unclear. We supposed the α(1)-AAs be correlated to the HLA-DQB1 and DRB1 alleles. Three hundred ninety-six patients with essential hypertension (EH) and 224 normotensives were enrolled, and DNA typing was detected by protein coupled receptor (PCR) amplification with sequence-specific primers. Analysis was performed by α(2) and logistic regression. There were the significant associations of the haplotype HLA-DQB1*0301-DRB1*04 with the prevalence of α(1)-AAs in hypertensive patients and it obviously added to the risk for the α(1)-AA production (adjusted P = 0.019, OR 4.037, 95% CI 1.259-12.947). In normotensives, the haplotype HLA-DQB1*05-DRB1*04 provided a strong predisposition in α (1)-AAs production (adjusted P = 0.024, OR 3.922, 95% CI 1.200-12.817). These results suggest the HLA-DRB1*04 might be the primary risk alleles associated with α(1)-AA production on the haplotypes HLA-DQB1*0301-DRB1*04 and HLA-DQB1*05-DRB1*04 and increased the risk for α (1)-AA production.
authors.affiliation: Laboratory of Cardiovascular Immunology, Institute of Cardiology, Union Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan, China.
authors.forename: Yanxiang
authors.initials: Y
authors.lastname: Sun
authors.forename: Feng
authors.initials: F
authors.lastname: Zhu
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.forename: Shihui
authors.initials: S
authors.lastname: Ma
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Liao
mesh_terms: D000328:Adult; D000368:Aged; D000483:Alleles; D000595:Amino Acid Sequence; D044466:Asian People / Q000235:genetics; D001323:Autoantibodies / Q000097:blood*; D016022:Case-Control Studies; D002681:China; D005260:Female; D020022:Genetic Predisposition to Disease; D055106:Genome-Wide Association Study; D006683:HLA-DQ Antigens / Q000235:genetics*; D059866:HLA-DQ beta-Chains; D006684:HLA-DR Antigens / Q000235:genetics*; D059811:HLA-DRB1 Chains; D006239:Haplotypes; D006801:Humans; D006973:Hypertension / Q000235:genetics* / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000235:genetics / Q000276:immunology; D011110:Polymorphism, Genetic; D018340:Receptors, Adrenergic, alpha-1 / Q000235:genetics / Q000276:immunology*; D012307:Risk Factors
chemical_list: D001323:Autoantibodies; D006683:HLA-DQ Antigens; D059866:HLA-DQ beta-Chains; C069051:HLA-DQB1 antigen; D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains; C527195:HLA-DRB1*04 antigen; D010446:Peptide Fragments; D018340:Receptors, Adrenergic, alpha-1