title: Identification of proteins overexpressed in papillary thyroid tumors.
abstract: A modified method of proteome comparative analysis based on preliminary removal of cell structural proteins by extraction using salt buffer and subsequent separation of extracts by two-dimensional gel electrophoresis was developed. Identification of differentially expressed proteins by mass spectrometry has revealed three proteins with noticeably increased level of synthesis in most samples of papillary thyroid tumors compared to normal tissues. An increase in ubiquitin content was found for the first time. Oncomarker search efficiencies by two-dimensional gel electrophoresis and bioinformatic search were compared.
authors.affiliation: Clinical Research Center PreMed, Moscow, 121357, Russia. larsipina@rambler.ru
authors.forename: L V
authors.initials: LV
authors.lastname: Sipina
authors.forename: Yu A
authors.initials: YA
authors.lastname: Bukurova
authors.forename: I G
authors.initials: IG
authors.lastname: Nikitina
authors.forename: G S
authors.initials: GS
authors.lastname: Krasnov
authors.forename: S A
authors.initials: SA
authors.lastname: Sergeev
authors.forename: N A
authors.initials: NA
authors.lastname: Lisitsyn
authors.forename: V L
authors.initials: VL
authors.lastname: Karpov
authors.forename: S F
authors.initials: SF
authors.lastname: Beresten
mesh_terms: D014408:Biomarkers, Tumor / Q000378:metabolism; D002277:Carcinoma; D002291:Carcinoma, Papillary; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D013058:Mass Spectrometry; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D000077273:Thyroid Cancer, Papillary; D013964:Thyroid Neoplasms / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; D020543:Proteome