title: Loss of the tumor suppressor Snf5 leads to aberrant activation of the Hedgehog-Gli pathway.
abstract: Aberrant activation of the Hedgehog (Hh) pathway can drive tumorigenesis. To investigate the mechanism by which glioma-associated oncogene family zinc finger-1 (GLI1), a crucial effector of Hh signaling, regulates Hh pathway activation, we searched for GLI1-interacting proteins. We report that the chromatin remodeling protein SNF5 (encoded by SMARCB1, hereafter called SNF5), which is inactivated in human malignant rhabdoid tumors (MRTs), interacts with GLI1. We show that Snf5 localizes to Gli1-regulated promoters and that loss of Snf5 leads to activation of the Hh-Gli pathway. Conversely, re-expression of SNF5 in MRT cells represses GLI1. Consistent with this, we show the presence of a Hh-Gli-activated gene expression profile in primary MRTs and show that GLI1 drives the growth of SNF5-deficient MRT cells in vitro and in vivo. Therefore, our studies reveal that SNF5 is a key mediator of Hh signaling and that aberrant activation of GLI1 is a previously undescribed targetable mechanism contributing to the growth of MRT cells.
authors.affiliation: Novartis Institutes for BioMedical Research, Cambridge, Massachusetts, USA.
authors.forename: Zainab
authors.initials: Z
authors.lastname: Jagani
authors.forename: E Lorena
authors.initials: EL
authors.lastname: Mora-Blanco
authors.forename: Courtney G
authors.initials: CG
authors.lastname: Sansam
authors.forename: Elizabeth S
authors.initials: ES
authors.lastname: McKenna
authors.forename: Boris
authors.initials: B
authors.lastname: Wilson
authors.forename: Dongshu
authors.initials: D
authors.lastname: Chen
authors.forename: Justin
authors.initials: J
authors.lastname: Klekota
authors.forename: Pablo
authors.initials: P
authors.lastname: Tamayo
authors.forename: Phuong T L
authors.initials: PT
authors.lastname: Nguyen
authors.forename: Michael
authors.initials: M
authors.lastname: Tolstorukov
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Park
authors.forename: Yoon-Jae
authors.initials: YJ
authors.lastname: Cho
authors.forename: Kathy
authors.initials: K
authors.lastname: Hsiao
authors.forename: Silvia
authors.initials: S
authors.lastname: Buonamici
authors.forename: Scott L
authors.initials: SL
authors.lastname: Pomeroy
authors.forename: Jill P
authors.initials: JP
authors.lastname: Mesirov
authors.forename: Heinz
authors.initials: H
authors.lastname: Ruffner
authors.forename: Tewis
authors.initials: T
authors.lastname: Bouwmeester
authors.forename: Sarah J
authors.initials: SJ
authors.lastname: Luchansky
authors.forename: Joshua
authors.initials: J
authors.lastname: Murtie
authors.forename: Joseph F
authors.initials: JF
authors.lastname: Kelleher
authors.forename: Markus
authors.initials: M
authors.lastname: Warmuth
authors.forename: William R
authors.initials: WR
authors.lastname: Sellers
authors.forename: Charles W M
authors.initials: CW
authors.lastname: Roberts
authors.forename: Marion
authors.initials: M
authors.lastname: Dorsch
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D047369:Chromatin Immunoprecipitation; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics / Q000378:metabolism*; D017931:DNA Primers / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D006801:Humans; D015151:Immunoblotting; D017403:In Situ Hybridization; D013058:Mass Spectrometry; D051379:Mice; D046228:Microarray Analysis; D018335:Rhabdoid Tumor / Q000235:genetics*; D000071796:SMARCB1 Protein; D015398:Signal Transduction / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D000071676:Zinc Finger Protein GLI1
chemical_list: D002868:Chromosomal Proteins, Non-Histone; D017931:DNA Primers; D004268:DNA-Binding Proteins; C400551:GLI1 protein, human; D000071796:SMARCB1 Protein; C513266:SMARCB1 protein, human; D014157:Transcription Factors; D000071676:Zinc Finger Protein GLI1