title: Effects of oriented substrates on cell morphology, the cell cycle, and the cytoskeleton in Ros 17/2.8 cells.
abstract: Absence of gravity or microgravity influences the cellular functions of bone forming osteoblasts. The underlying mechanism, however, of cellular sensing and responding to the gravity vector is poorly understood. This work quantified the impact of vector-directional gravity on the biological responses of Ros 17/2.8 cells grown on upward-, downward- or edge-on-oriented substrates. Cell morphology and nuclear translocation, cell proliferation and the cell cycle, and cytoskeletal reorganization were found to vary significantly in the three orientations. All of the responses were duration-dependent. These results provide a new insight into understanding how osteoblasts respond to static vector-directional gravity.
authors.affiliation: Key Laboratory of Microgravity, Institute of Mechanics, Chinese Academy of Sciences, Beijing, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Li
authors.forename: Juan
authors.initials: J
authors.lastname: Chen
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: ShuJin
authors.initials: S
authors.lastname: Sun
authors.forename: ZuLai
authors.initials: Z
authors.lastname: Tao
authors.forename: Mian
authors.initials: M
authors.lastname: Long
mesh_terms: D021581:Active Transport, Cell Nucleus; D000818:Animals; D018929:Cell Culture Techniques; D002453:Cell Cycle / Q000502:physiology*; D002460:Cell Line; D049109:Cell Proliferation; D048430:Cell Shape; D003599:Cytoskeleton / Q000502:physiology*; D006112:Gravitation; D006801:Humans; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology*; D051381:Rats