title: Microextraction by packed sorbent for LC-MS/MS determination of drugs in whole blood samples.
abstract: BACKGROUND
  Microextraction by packed sorbent (MEPS) is used as an online sample-preparation method. The determination of local anesthetics lidocaine, ropivacaine and bupivacaine directly in human blood was performed using MEPS online with LC-MS/MS.
  
  
  RESULTS
  The range of the calibration curves in whole blood was 10-10000 nmol/l. The lower limit of quantification was set to 10.0 nmol/l. The accuracy of the quality control samples ranged from 85 to 97%. The interday precision of the studied analytes was within the range 1-5%. The regression correlation coefficient (r(2)) was over 0.995 for all runs. The present method is rapid, reliable and robust and may be used for therapeutic drug monitoring of studied analytes in whole blood.
  
  
  CONCLUSIONS
  This assay allows the analysis of drugs in human blood directly. Sample preparation is simple and automated. The assay reduced the handling time and the cost, and could handle small volumes of whole blood samples (25 µl).
authors.affiliation: Department of Clinical Pharmacology, Huddinge University Hospital, 17176 Stockholm, Sweden.
authors.forename: Rana
authors.initials: R
authors.lastname: Said
authors.forename: Mohamed
authors.initials: M
authors.lastname: Kamel
authors.forename: Aziza
authors.initials: A
authors.lastname: El-Beqqali
authors.forename: Mohamed
authors.initials: M
authors.lastname: Abdel-Rehim
mesh_terms: D053000:Analytic Sample Preparation Methods / Q000379:methods*; D001774:Blood Chemical Analysis / Q000379:methods*; D002138:Calibration; D005591:Chemical Fractionation / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D004364:Pharmaceutical Preparations / Q000097:blood* / Q000302:isolation & purification*; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D004364:Pharmaceutical Preparations