title: [Effects of electroacupuncture of "Weishu" (BL 21) and "Zhongwan" (CV 12) on serum large molecular metabolites in functional dyspepsia rats].
abstract: OBJECTIVE
  To compare the effects of electroacupuncture (EA) of Shu-acupoint "Weishu" (BL 21), Mu-acupoint "Zhongwan" (CV 12) and BL 21 + CV 12 on serum large molecular metabolites in functional dyspepsia (FD) rats.
  
  
  METHODS
  A total of 50 Wistar rats were randomly divided into control, model, BL 21, CV 12 and BL 21 + CV 12 groups (n = 10/group). FD model was established by clamping the rats' tails for 30 min, once every 3 h, 4 times daily for 7 days. EA was applied to BL 21,CV 12, or BL 21 + CV 12, respectively for 20 min, once daily for 10 days. The rats' venous blood was collected for detecting large molecular metabolites by 1H nuclear magnetic resonance (NMR) spectrometer (Varian UnityInova, 600 MHz). Segmental integral calculus (0.04 ppm per segment) was performed from 6.0-0 ppm (longitudinal eddy-delay, LED) as defaulted 1H spectra values by using the VNMR software. Data was saved as text or excel files after normalization and then used for pattern recognition analyses. All the data were analyzed by principal component analysis (PCA) using the SIMCA-P 10.04 software (Umetrics AB, Umea, Sweden), and orthogonal signal correction and partial least squares discriminant analysis (OSC-PLS-DA).
  
  
  RESULTS
  The rat serum proton-LED-1H NMR spectra revealed different metabolic spectra among control,model, BL 21, CV 12, and BL 21 + CV 12 groups. PCA and OSC-PLS-DA showed that compared with the normal control group, FD rats in model group displayed serum N-acetylglucoprotein (NAc) content was reduced considerably, high density lipoprotein (HDL) level decreased to a certain degree, while very low density lipoprotein (VLDL)/LDL increased significantly. In comparison with model group, VLDL/LDL was decreased in BL 21, CV 12 and BL 21 + CV 12 groups, while NAc content increased. Compared with BL 21 and CV 12 groups respectively, HDL level in BL 21 + CV 12 increased to a certain degree.
  
  
  CONCLUSIONS
  EA of BL 21, CV 12 and BL 21 + CV 12 groups can reverse or relieve FD induced increase of VLDL/LDL and decrease of serum NAc level in rats, which may contribute to its function in improving symptoms of FD patients.
authors.affiliation: College of Acu-moxibustion and Tuina, Chengdu University of Chinese Medicine, Chengdu 610075, China. qiaofengwu@yahoo.cn
authors.forename: Qiao-Feng
authors.initials: QF
authors.lastname: Wu
authors.forename: Sen
authors.initials: S
authors.lastname: Mao
authors.forename: Wei
authors.initials: W
authors.lastname: Cai
authors.forename: Xian-Zhong
authors.initials: XZ
authors.lastname: Yan
authors.forename: Ji-Lan
authors.initials: JL
authors.lastname: Zhao
authors.forename: Shu-Guang
authors.initials: SG
authors.lastname: Yu
authors.forename: Yong
authors.initials: Y
authors.lastname: Tang
mesh_terms: D015669:Acupuncture Points; D000818:Animals; D004195:Disease Models, Animal; D004415:Dyspepsia / Q000097:blood* / Q000000981:diagnostic imaging / Q000628:therapy*; D015671:Electroacupuncture; D006801:Humans; D008074:Lipoproteins / Q000097:blood*; D008279:Magnetic Resonance Imaging; D008297:Male; D011859:Radiography; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
chemical_list: D008074:Lipoproteins