title: Aspirin increases apolipoprotein-A-I-mediated cholesterol efflux via enhancing expression of ATP-binding cassette transporter A1.
abstract: BACKGROUND
  The efflux of cellular cholesterol mediated by apolipoprotein (apo)A-I and ATP-binding cassette transporter A1 (ABCA1) is a major pathway of reverse cholesterol transport. We investigated the effect of aspirin on this process.
  
  
  METHODS
  The expression levels of ABCA1 in RAW264.7 cells were determined using reverse transcription polymerase chain reaction and Western blot analysis. ³H-cholesterol efflux was measured by scintillation counting.
  
  
  RESULTS
  0.5 mmol/l aspirin increased apoA-I-mediated cholesterol efflux and increased the expression of ABCA1. By increasing the dose of aspirin higher than 0.5 mmol/l, ABCA1 expression and function were significantly decreased. In cells transfected with a specific peroxisome proliferator-activated receptor (PPAR)-α small interfering RNA, the induction of ABCA1 expression and apoA-I-mediated ³H-cholesterol efflux by aspirin were substantially suppressed.
  
  
  CONCLUSIONS
  The data demonstrate that low-dose aspirin increases ABCA1 expression via a PPAR-α-dependent mechanism and increases apoA-I-mediated cholesterol efflux.
authors.affiliation: Laboratory of Pharmacology and Toxicology, School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou, China.
authors.forename: Yu-Hua
authors.initials: YH
authors.lastname: Wang
authors.forename: Yan-Fang
authors.initials: YF
authors.lastname: Chen
authors.forename: Shao-Rui
authors.initials: SR
authors.lastname: Chen
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.forename: Jian-Wen
authors.initials: JW
authors.lastname: Chen
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Shen
authors.forename: Yong-Gao
authors.initials: YG
authors.lastname: Mou
authors.forename: Pei-Qing
authors.initials: PQ
authors.lastname: Liu
mesh_terms: D064286:ATP Binding Cassette Transporter 1; D018528:ATP-Binding Cassette Transporters / Q000235:genetics*; D000818:Animals; D016632:Apolipoprotein A-I / Q000378:metabolism*; D001241:Aspirin / Q000494:pharmacology*; D015153:Blotting, Western; D002478:Cells, Cultured; D002784:Cholesterol / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects; D051379:Mice; D047493:PPAR alpha / Q000378:metabolism; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology; D034741:RNA, Small Interfering / Q000008:administration & dosage; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012588:Scintillation Counting; D014162:Transfection
chemical_list: D064286:ATP Binding Cassette Transporter 1; D018528:ATP-Binding Cassette Transporters; D016632:Apolipoprotein A-I; D047493:PPAR alpha; D010975:Platelet Aggregation Inhibitors; D034741:RNA, Small Interfering; D002784:Cholesterol; D001241:Aspirin