title: The generation and the manipulation of human multipotent adipose-derived stem cells.
abstract: In this chapter, we describe a method to isolate and to expand multipotent adipose-derived stem (hMADS) cells from human adipose tissue. We also describe culture conditions to differentiate them into adipocytes at a high rate. This culture system provides a powerful means for studying the first steps of human adipose cell development and a route for investigating effects of drugs on the biology of adipocytes. Finally, we provide a protocol to investigate gene function during proliferation and differentiation of hMADS cells by means of siRNA-mediated gene silencing approaches or forced expression by transducing hMADS cells permissive to infection with murine retrovirus vectors.
authors.affiliation: Institut de Recherche, Signalisation, Biologie du Développement et Cancer, UMR6543 Centre National de la Recherche Scientifique (CNRS), Université Nice Sophia-Antipolis, Nice, France.
authors.forename: Brigitte
authors.initials: B
authors.lastname: Wdziekonski
authors.forename: Tala
authors.initials: T
authors.lastname: Mohsen-Kanson
authors.forename: Phi
authors.initials: P
authors.lastname: Villageois
authors.forename: Christian
authors.initials: C
authors.lastname: Dani
mesh_terms: D017667:Adipocytes / Q000166:cytology; D000273:Adipose Tissue / Q000166:cytology*; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D049109:Cell Proliferation; D002469:Cell Separation; D048430:Cell Shape; D005615:Freezing; D020868:Gene Silencing; D006801:Humans; D039902:Multipotent Stem Cells / Q000166:cytology* / Q000378:metabolism / Q000821:virology; D009584:Nitrogen; D034741:RNA, Small Interfering / Q000378:metabolism; D012190:Retroviridae / Q000235:genetics; D014162:Transfection
chemical_list: D034741:RNA, Small Interfering; D009584:Nitrogen