title: The effects of topical application of bisphosphonates on replanted rat molars.
abstract: The purpose of this study was to evaluate the potential usefulness of two bisphosphonates (BPs) (etidronate and zoledronate), compared with that of alendronate, which is a well-known drug for delayed replantation, in decreasing or preventing inflammatory root resorption and replacement root resorption in replanted teeth. Eighty-four Sprague Dawley rat maxillary first molars were extracted, dried for 60 min and then replanted after root treatment. The rats were divided into four groups (control, alendronate, etidronate, zoledronate) as following treatments of avulsed root before replantation. At 7, 14, and 28 days postreplantation, the animals were sacrificed and the samples obtained and process for microscopic analysis. The data were statistically analyzed with the SPSS procedure, anova test and each test was complemented by the Tukey's post hoc test. The result indicated that topical application of alendronate and zoledronate, both nitrogen containing BPs, prevented inflammatory root resorption and inflammatory cell response in the delayed replantation model. Both drugs were demonstrated similar effects in the delayed tooth replantation model (P = 0.9). Etidronate did not prevent inflammatory root resorption and inflammation in the delayed replantation (P < 0.05). No significant differences in replacement root resorption were observed among all drugs. These results suggest that when teeth are dried and not replanted immediately, zoledronate, like alendronate, may prevent root resorption and facilitates the regeneration of periodontal tissues after replantation.
authors.affiliation: Department of Pediatric Dentistry and Institute of Oral Biology, School of Dentistry, Kyung Hee University, Gangdong-gu, Seoul, Korea.
authors.forename: Sung Chul
authors.initials: SC
authors.lastname: Choi
authors.forename: Yong-Dae
authors.initials: YD
authors.lastname: Kwon
authors.forename: Kwang Chul
authors.initials: KC
authors.lastname: Kim
authors.forename: Gue-Tae
authors.initials: GT
authors.lastname: Kim
mesh_terms: D000287:Administration, Topical; D019386:Alendronate / Q000008:administration & dosage; D000818:Animals; D050071:Bone Density Conservation Agents / Q000008:administration & dosage*; D019343:Citric Acid / Q000627:therapeutic use; D003739:Dental Cementum / Q000473:pathology; D003804:Dentin / Q000473:pathology; D003890:Desiccation; D004164:Diphosphonates / Q000008:administration & dosage*; D012968:Etidronic Acid / Q000008:administration & dosage; D007093:Imidazoles / Q000008:administration & dosage; D008297:Male; D008963:Molar / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D012390:Root Canal Therapy; D012391:Root Resorption / Q000145:classification / Q000517:prevention & control*; D013696:Temperature; D013997:Time Factors; D014090:Tooth Replantation / Q000379:methods*; D014092:Tooth Root / Q000473:pathology; D020390:Tooth Socket / Q000601:surgery; D000077211:Zoledronic Acid
chemical_list: D050071:Bone Density Conservation Agents; D004164:Diphosphonates; D007093:Imidazoles; D019343:Citric Acid; D000077211:Zoledronic Acid; D012968:Etidronic Acid; D019386:Alendronate