title: Modulatory effect of coffee on platelet function.
abstract: Blood platelets play a major role in cardiovascular disease (CVD) and thrombosis. Conflicting information exists regarding the effect of coffee consumption on the cardiovascular system. We have investigated whether the consumption of moderate amount of coffee affect platelet functions and primary hemostasis in vivo in normal and high fat diet fed rats. Coffee fed group showed significant (P < 0.05) decrease in mean platelet volume, platelet crit and platelet distribution width as compared to high fat diet (HFD) group. The concentration of malondialdehyde in platelets increased in atherosclerotic group indicates the increased thromboxane A2 (TXA2) production from membrane arachidonic acid and it was decreased in coffee treated group. Platelet aggregation studies with ADP, collagen, arachidonic acid and epinephrine showed significant (P < 0.05) decrease in aggregation in coffee fed group. Scanning electron microscopic studies revealed that platelet aggregation tendency increased in HFD group and was reduced in coffee fed group. These results indicate that coffee is active in inhibiting platelet aggregation, a critical step involved in thrombosis.
authors.affiliation: Department of Biochemistry, University of Kerala, Kariavattom, Thiruvananthapuram.
authors.forename: Shobha
authors.initials: S
authors.lastname: Bhaskar
authors.forename: Arun A
authors.initials: AA
authors.lastname: Rauf
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000378:metabolism; D002791:Cholesterol, Dietary; D003069:Coffee; D004195:Disease Models, Animal; D005260:Female; D055951:Functional Food; D006487:Hemostasis; D006937:Hypercholesterolemia / Q000097:blood* / Q000209:etiology; D008315:Malondialdehyde / Q000097:blood; D008855:Microscopy, Electron, Scanning; D010974:Platelet Aggregation; D010979:Platelet Function Tests; D051381:Rats; D017207:Rats, Sprague-Dawley; D013928:Thromboxane A2 / Q000097:blood; D013997:Time Factors
chemical_list: D002791:Cholesterol, Dietary; D003069:Coffee; D008315:Malondialdehyde; D013928:Thromboxane A2