title: Simultaneous qualification and quantification of baccharane glycosides in Impatientis Semen by HPLC-ESI-MSD and HPLC-ELSD.
abstract: This study presents a high performance liquid chromatography (HPLC) with electrospray ionization mass spectrometric detection (ESI-MSD) and evaporative light scattering detection (ELSD) method for the simultaneous qualification and quantification of eight major baccharane glycosides, namely hosenlosides A, B, C, F, G, K, L, and M in Impatientis Semen, a Chinese herbal medicine derived from the seeds of Impatiens balsamina L. In order to achieve optimum performance, several extraction parameters (including extraction solvent, extraction mode, extraction time) were optimized. The baccharane glycosides were separated on a Shim-pack CLC-ODS column with gradient elution of water and methanol. Temperature for the ELSD drift tube was set at 98°C and the nitrogen flow rate was 2.7l/min. The unambiguous identities of the analytes were realized by comparing retention times and mass data with those of reference compounds. The developed method was fully validated in terms of linearity, sensitivity, precision, repeatability, recovery as well as robustness, and subsequently applied to evaluate the quality of 14 batches of Impatientis Semen commercial samples from different collections.
authors.affiliation: Key Laboratory of Modern Chinese Medicines, China Pharmaceutical University, Ministry of Education, No. 24 Tongjia Lane, Nanjing, China.
authors.forename: Hui-Jun
authors.initials: HJ
authors.lastname: Li
authors.forename: Jun-Jie
authors.initials: JJ
authors.lastname: Yu
authors.forename: Ping
authors.initials: P
authors.lastname: Li
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D006027:Glycosides / Q000032:analysis* / Q000302:isolation & purification; D029069:Impatiens / Q000737:chemistry*; D057230:Limit of Detection; D009682:Magnetic Resonance Spectroscopy; D009391:Nephelometry and Turbidimetry; D011786:Quality Control; D015203:Reproducibility of Results; D012503:Saponins / Q000032:analysis* / Q000737:chemistry; D012639:Seeds / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D013678:Technology, Pharmaceutical
chemical_list: D004365:Drugs, Chinese Herbal; D006027:Glycosides; D012503:Saponins; C557483:Impatientis Semen