title: Bioanalytical and instrumental analysis of thyroid hormone disrupting compounds in water sources along the Yangtze River.
abstract: Thyroid hormone (TH) agonist and antagonist activities of water sources along the Yangtze River in China were surveyed by a green monkey kidney fibroblast (CV-1) cell-based TH reporter gene assay. Instrumental analysis was conducted to identify the responsible thyroid-active compounds. Instrumentally derived l-3,5,3'-triiodothyronine (T(3)) equivalents (T(3)-EQs) and thyroid receptor (TR) antagonist activity equivalents referring to dibutyl phthalate (DBP-EQs) were calculated from the concentrations of individual congeners. The reporter gene assay demonstrated that three out of eleven water sources contained TR agonist activity equivalents (TR-EQs), ranging from 286 to 293 ng T(3)/L. Anti-thyroid hormone activities were found in all water sources with the TR antagonist activity equivalents referring to DBP (Ant-TR-EQs), ranging from 51.5 to 555.3 μg/L. Comparisons of the equivalents from instrumental and biological assays suggested that high concentrations of DBP and di-2-ethylhexyl phthalate (DEHP) were responsible for the observed TR antagonist activities at some locations along the Yangtze River.
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210093, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Shi
authors.forename: Xiaoyi
authors.initials: X
authors.lastname: Wang
authors.forename: Guanjiu
authors.initials: G
authors.lastname: Hu
authors.forename: Yingqun
authors.initials: Y
authors.lastname: Hao
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Zhang
authors.forename: Hongling
authors.initials: H
authors.lastname: Liu
authors.forename: Si
authors.initials: S
authors.lastname: Wei
authors.forename: Xinru
authors.initials: X
authors.lastname: Wang
authors.forename: Hongxia
authors.initials: H
authors.lastname: Yu
mesh_terms: D000818:Animals; D013956:Antithyroid Agents / Q000032:analysis*; D001681:Biological Assay / Q000295:instrumentation / Q000379:methods; D002460:Cell Line; D002681:China; D002522:Chlorocebus aethiops; D052244:Endocrine Disruptors / Q000032:analysis*; D017930:Genes, Reporter; D011988:Receptors, Thyroid Hormone / Q000032:analysis / Q000037:antagonists & inhibitors; D013963:Thyroid Hormones / Q000819:agonists* / Q000032:analysis; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D013956:Antithyroid Agents; D052244:Endocrine Disruptors; D011988:Receptors, Thyroid Hormone; D013963:Thyroid Hormones; D014874:Water Pollutants, Chemical