title: Single molecule atomic force microscopy studies of photosensitized singlet oxygen behavior on a DNA origami template.
abstract: DNA origami, the folding of a long single-stranded DNA sequence (scaffold strand) by hundreds of short synthetic oligonucleotides (staple strands) into parallel aligned helices, is a highly efficient method to form advanced self-assembled DNA-architectures. Since molecules and various materials can be conjugated to each of the short staple strands, the origami method offers a unique possibility of arranging molecules and materials in well-defined positions on a structured surface. Here we combine the action of light with AFM and DNA nanostructures to study the production of singlet oxygen from a single photosensitizer molecule conjugated to a selected DNA origami staple strand on an origami structure. We demonstrate a distance-dependent oxidation of organic moieties incorporated in specific positions on DNA origami by singlet oxygen produced from a single photosensitizer located at the center of each origami.
authors.affiliation: Center for DNA Nanotechnology at the Interdisciplinary Nanoscience Center, Aarhus University, DK-8000 Aarhus C, Denmark.
authors.forename: Sarah
authors.initials: S
authors.lastname: Helmig
authors.forename: Alexandru
authors.initials: A
authors.lastname: Rotaru
authors.forename: Dumitru
authors.initials: D
authors.lastname: Arian
authors.forename: Larisa
authors.initials: L
authors.lastname: Kovbasyuk
authors.forename: Jacob
authors.initials: J
authors.lastname: Arnbjerg
authors.forename: Peter R
authors.initials: PR
authors.lastname: Ogilby
authors.forename: Jørgen
authors.initials: J
authors.lastname: Kjems
authors.forename: Andriy
authors.initials: A
authors.lastname: Mokhir
authors.forename: Flemming
authors.initials: F
authors.lastname: Besenbacher
authors.forename: Kurt V
authors.initials: KV
authors.lastname: Gothelf
mesh_terms: D004247:DNA / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D008958:Models, Molecular; D049329:Nanostructures / Q000737:chemistry; D009690:Nucleic Acid Conformation; D017319:Photosensitizing Agents / Q000494:pharmacology*; D026082:Singlet Oxygen / Q000737:chemistry*
chemical_list: D017319:Photosensitizing Agents; D026082:Singlet Oxygen; D004247:DNA