title: Molecular and biological characterization of pepper yellow vein Mali virus (PepYVMV) isolates associated with pepper yellow vein disease in Burkina Faso.
abstract: Yellow vein disease (YVD) is a major problem in pepper in West Africa. Despite the recent implication of a begomovirus in YVD in Mali and in Burkina Faso, the aetiology of the disease remains unclear. Using symptomatic samples from the main vegetable cultivation regions in Burkina Faso, 10 full-length DNA-A-like begomovirus sequences were obtained, each showing 98% nucleotide identity to pepper yellow vein Mali virus (PepYVMV). The host range was determined after construction of a viral clone for agroinfection. Severe symptoms developed in tomato and Nicotiana benthamiana. By contrast, no symptoms developed in either commercial or local pepper cultivars, demonstrating that the aetiology of YVD is not only associated with the presence of PepYVMV.
authors.affiliation: Laboratoire de Biochimie & Biologie Moléculaire, CRSBAN/UFR/SVT, Université de Ouagadougou, 03 BP 7021 Ouagadougou 03, Burkina Faso. fidelet@gmail.com
authors.forename: Fidèle
authors.initials: F
authors.lastname: Tiendrébéogo
authors.forename: Pierre
authors.initials: P
authors.lastname: Lefeuvre
authors.forename: Murielle
authors.initials: M
authors.lastname: Hoareau
authors.forename: Valentin S Edgar
authors.initials: VS
authors.lastname: Traoré
authors.forename: Nicolas
authors.initials: N
authors.lastname: Barro
authors.forename: Frédéric
authors.initials: F
authors.lastname: Péréfarres
authors.forename: Bernard
authors.initials: B
authors.lastname: Reynaud
authors.forename: Alfred S
authors.initials: AS
authors.lastname: Traoré
authors.forename: Gnissa
authors.initials: G
authors.lastname: Konaté
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Lett
authors.forename: Oumar
authors.initials: O
authors.lastname: Traoré
mesh_terms: D053839:Begomovirus / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity*; D002050:Burkina Faso; D002212:Capsicum / Q000821:virology*; D016000:Cluster Analysis; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D058507:Host Specificity; D018551:Solanum lycopersicum / Q000821:virology; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014026:Nicotiana / Q000821:virology
chemical_list: D004279:DNA, Viral