title: Differential gene expression in mutant mice overexpressing or deficient in the serotonin transporter: a focus on urocortin 1.
abstract: Transcriptome analyses were performed in the anterior raphe area of mutant mice deficient in the serotonin transporter (5-HTT KO) or overexpressing this protein (5-HTT TG), which exhibit opposite changes in anxiety-related behavior. Among genes with altered expression, the gene encoding the neuropeptide urocortin 1 was down-regulated in 5-HTT KO and up-regulated in 5-HTT TG mice. Expression of the gene encoding cocaine-and-amphetamine-related-peptide, which colocalizes with urocortin 1, was also increased in 5-HTT TG mutants. Real-time RT-PCR confirmed these data and immunoautoradiographic labeling showed that parallel changes in neuropeptide levels were confined to the non-preganglionic Edinger-Westphal nucleus. Thus, 5-HTT expression correlates with that of urocortin 1, suggesting that this peptide can be involved in the behavioral changes observed in 5-HTT mutant mice.
authors.affiliation: Université Pierre et Marie Curie-Paris6, Faculté de Médecine Pierre et Marie Curie, Site Pitié-Salpêtrière, IFR 70 des Neurosciences, UMR S677, Paris, F-75013, France. veronique.fabre@upmc.fr
authors.forename: Véronique
authors.initials: V
authors.lastname: Fabre
authors.forename: Renaud
authors.initials: R
authors.lastname: Massart
authors.forename: Adeline
authors.initials: A
authors.lastname: Rachalski
authors.forename: Katie
authors.initials: K
authors.lastname: Jennings
authors.forename: Andrew
authors.initials: A
authors.lastname: Brass
authors.forename: Trevor
authors.initials: T
authors.lastname: Sharp
authors.forename: Klaus-Peter
authors.initials: KP
authors.lastname: Lesch
authors.forename: Laurence
authors.initials: L
authors.lastname: Lanfumey
authors.forename: Michel
authors.initials: M
authors.lastname: Hamon
mesh_terms: D000818:Animals; D001007:Anxiety / Q000235:genetics* / Q000378:metabolism; D001345:Autoradiography; D001522:Behavior, Animal; D003866:Depressive Disorder / Q000235:genetics* / Q000378:metabolism; D004195:Disease Models, Animal; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D046228:Microarray Analysis; D009419:Nerve Tissue Proteins / Q000172:deficiency / Q000235:genetics / Q000378:metabolism; D011903:Raphe Nuclei / Q000378:metabolism; D050486:Serotonin Plasma Membrane Transport Proteins / Q000172:deficiency / Q000235:genetics* / Q000378:metabolism; D054832:Urocortins / Q000235:genetics* / Q000378:metabolism; D000098882:Cocaine- and Amphetamine-Regulated Transcript Protein
chemical_list: D009419:Nerve Tissue Proteins; D050486:Serotonin Plasma Membrane Transport Proteins; D054832:Urocortins; D000098882:Cocaine- and Amphetamine-Regulated Transcript Protein