title: Patterns of gene expression in muscle and fat in tumor-bearing rats: effects of CRF2R agonist on cachexia.
abstract: The hypothesis we tested was that administering corticotropin-releasing factor receptor agonists preserves muscle mass during cancer that is related to changes in tissue gene expression. cDNA microarrays were used to compare mRNAs from muscle and adipose tissues of non-treated and agonist-treated tumor-bearing rats. In muscle of non-tumor-bearing agonist-treated animals we observed decreased expression of genes associated with fatty acid uptake and esterification. In tumor-bearing animals, CRF2R agonist administration produced decreased mRNA content of the atrogene lipin-1. In white adipose tissue, agonist treatment of non-tumor-bearing animals induced genes typically related to muscle structure and function. The fact that this treatment decreased expression of atrogenes could have clinical application. In addition, agonist treatment changed the gene pattern of adipose tissue to render it similar to that of skeletal muscle; thus, treatment with this agonist alters the gene pattern to what could be called "muscularization of white adipose tissue."
authors.affiliation: Cancer Research Group, Departament de Bioquímica i Biologia Molecular, Facultat de Biologia, Universitat de Barcelona, Diagonal 645, Barcelona 08028, Spain.
authors.forename: Josep M
authors.initials: JM
authors.lastname: Argilés
authors.forename: Cibely Cristine
authors.initials: CC
authors.lastname: Fontes-Oliveira
authors.forename: Gemma
authors.initials: G
authors.lastname: Fuster
authors.forename: Elisabet
authors.initials: E
authors.lastname: Ametller
authors.forename: Maite
authors.initials: M
authors.lastname: Figueras
authors.forename: Mireia
authors.initials: M
authors.lastname: Olivan
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Lopez-Soriano
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Qu
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Demuth
authors.forename: Paula
authors.initials: P
authors.lastname: Stevens
authors.forename: Alex
authors.initials: A
authors.lastname: Varbanov
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Isfort
authors.forename: Sílvia
authors.initials: S
authors.lastname: Busquets
mesh_terms: D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism*; D000704:Analysis of Variance; D000818:Animals; D002100:Cachexia / Q000235:genetics / Q000378:metabolism*; D003346:Corticotropin-Releasing Hormone / Q000378:metabolism / Q000494:pharmacology*; D015870:Gene Expression; D008297:Male; D018482:Muscle, Skeletal / Q000187:drug effects / Q000378:metabolism*; D009368:Neoplasm Transplantation; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D018019:Receptors, Corticotropin-Releasing Hormone / Q000819:agonists*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D046888:Tissue Array Analysis
chemical_list: C101531:CRF receptor type 2; C530902:Lpin1 protein, rat; D009687:Nuclear Proteins; D012333:RNA, Messenger; D018019:Receptors, Corticotropin-Releasing Hormone; D003346:Corticotropin-Releasing Hormone