title: Ozonation and activated carbon treatment of sewage effluents: removal of endocrine activity and cytotoxicity.
abstract: Concerns about endocrine disrupting compounds in sewage treatment plant (STP) effluents give rise to the implementation of advanced treatment steps for the elimination of trace organic contaminants. The present study investigated the effects of ozonation (O(3)) and activated carbon treatment (AC) on endocrine activities [estrogenicity, anti-estrogenicity, androgenicity, anti-androgenicity, aryl-hydrocarbon receptor (AhR) agonistic activity] with yeast-based bioassays. To evaluate the removal of non-specific toxicity, a cytotoxicity assay using a rat cell line was applied. Wastewater (WW) was sampled at two STPs after conventional activated sludge treatment following the secondary clarifier (SC) and after subsequent advanced treatments: O(3), O(3) + sand filtration (O(3-SF)), and AC. Conventional treatment reduced estrogenicity, androgenicity, and AhR agonistic activity by 78-99% compared to the untreated influent WW. Anti-androgenicity and anti-estrogenicity were not detectable in the influent but appeared in SC, possibly due to the more effective removal of respective agonists during conventional treatment. Endocrine activities after SC ranged from 2.0 to 2.8 ng/L estradiol equivalents (estrogenicity), from 4 to 22 μg/L 4-hydroxytamoxifen equivalents (anti-estrogenicity), from 1.9 to 2.0 ng/L testosterone equivalents (androgenicity), from 302 to 614 μg/L flutamide equivalents (anti-androgenicity), and from 387 to 741 ng/L β-naphthoflavone equivalents (AhR agonistic activity). In particular, estrogenicity and anti-androgenicity occurred in environmentally relevant concentrations. O(3) and AC further reduced endocrine activities effectively (estrogenicity: 77-99%, anti-androgenicity: 63-96%, AhR agonistic activity: 79-82%). The cytotoxicity assay exhibited a 32% removal of non-specific toxicity after O(3) compared to SC. O(3) and sand filtration reduced cytotoxic effects by 49%, indicating that sand filtration contributes to the removal of toxicants. AC was the most effective technology for cytotoxicity removal (61%). Sample evaporation reduced cytotoxic effects by 52 (AC) to 73% (O(3)), demonstrating that volatile substances contribute considerably to toxic effects, particularly after O(3). These results confirm an effective removal or transformation of toxicants with receptor-mediated mode of action and non-specific toxicants during O(3) and AC. However, due to the limited extractability, polar ozonation by-products were neglected for toxicity analysis, and hence non-specific toxicity after O(3) is underestimated.
authors.affiliation: Goethe University Frankfurt am Main, Biological Sciences Division, Department Aquatic Ecotoxicology, Siesmayerstrasse 70, 60323 Frankfurt, Germany. stalter@bio.uni-frankfurt.de
authors.forename: Daniel
authors.initials: D
authors.lastname: Stalter
authors.forename: Axel
authors.initials: A
authors.lastname: Magdeburg
authors.forename: Martin
authors.initials: M
authors.lastname: Wagner
authors.forename: Jörg
authors.initials: J
authors.lastname: Oehlmann
mesh_terms: D000818:Animals; D002460:Cell Line; D002606:Charcoal; D052244:Endocrine Disruptors / Q000302:isolation & purification* / Q000633:toxicity; D010126:Ozone; D051381:Rats; D012722:Sewage; D018675:Toxicity Tests; D014865:Waste Disposal, Fluid / Q000379:methods*; D014874:Water Pollutants, Chemical / Q000302:isolation & purification / Q000633:toxicity
chemical_list: D052244:Endocrine Disruptors; D012722:Sewage; D014874:Water Pollutants, Chemical; D002606:Charcoal; D010126:Ozone