title: Expression of transient receptor potential vanilloid-1 (TRPV1) in urothelial cancers of human bladder: relation to clinicopathological and molecular parameters.
abstract: OBJECTIVE
  To evaluate the expression of transient receptor potential vanilloid type-1 channel protein (TRPV1) in normal and neoplastic urothelial tissues and to correlate TRPV1 expression with clinicopathological parameters and disease-specific survival.
  
  
  RESULTS
  TRPV1 expression was analysed in normal and neoplastic urothelial samples at both mRNA and protein levels by quantitative real time polymerase chain reaction (qPCR) and immunohistochemistry, respectively. TRPV1 downregulation was found in urothelial cancer (UC) specimens, which correlated with tumour progression. Moreover, TRPV1 mRNA levels were associated with clinicopathological parameters to assess the role of TRPV1 downregulation as a negative prognostic factor for survival. Kaplan-Meier survival analysis demonstrated a significantly shorter survival in patients showing TRPV1 mRNA downregulation. Multivariate Cox regression analysis indicated further that TRPV1 mRNA expression retained its significance as an independent risk factor.
  
  
  CONCLUSIONS
  The progression of UC of human bladder is associated with a marked decrease in TRPV1 expression, with a progressive loss in high-grade muscle invasive UC. Downregulation of TRPV1 mRNA expression may represent an independent negative prognostic factor for bladder cancer patients.
authors.affiliation: School of Pharmacy, University of Camerino, Camerino (MC), Italy.
authors.forename: Cristina
authors.initials: C
authors.lastname: Kalogris
authors.forename: Sara
authors.initials: S
authors.lastname: Caprodossi
authors.forename: Consuelo
authors.initials: C
authors.lastname: Amantini
authors.forename: Federica
authors.initials: F
authors.lastname: Lambertucci
authors.forename: Massimo
authors.initials: M
authors.lastname: Nabissi
authors.forename: Maria Beatrice
authors.initials: MB
authors.lastname: Morelli
authors.forename: Valerio
authors.initials: V
authors.lastname: Farfariello
authors.forename: Alessandra
authors.initials: A
authors.lastname: Filosa
authors.forename: Maria Cristina
authors.initials: MC
authors.lastname: Emiliozzi
authors.forename: Gabriele
authors.initials: G
authors.lastname: Mammana
authors.forename: Giorgio
authors.initials: G
authors.lastname: Santoni
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D018450:Disease Progression; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D009367:Neoplasm Staging; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D050916:TRPV Cation Channels / Q000235:genetics / Q000378:metabolism*; D001743:Urinary Bladder / Q000378:metabolism / Q000473:pathology*; D001749:Urinary Bladder Neoplasms / Q000235:genetics / Q000401:mortality / Q000473:pathology*; D019459:Urothelium / Q000378:metabolism / Q000473:pathology
chemical_list: D012333:RNA, Messenger; D050916:TRPV Cation Channels; C494482:TRPV1 protein, human