title: 12-oxo-phytodienoic acid-glutathione conjugate is transported into the vacuole in Arabidopsis.
abstract: While exogenous toxic compounds such as herbicides are thought to be sequestered into vacuoles in the form of glutathione (GSH) conjugates, little is understood about natural plant products conjugated with GSH. To identify natural products conjugated with GSH in plants, metabolites in the Arabidopsis γ-glutamyl transpeptidase (ggt) 4 knockout mutants that are blocked in the degradation of GSH conjugates in the vacuole were compared with those in wild-type plants. Among the metabolites identified, one was confirmed to be the 12-oxo-phytodienoic acid (OPDA)-GSH conjugate, indicating that OPDA, a precursor of jasmonic acid (JA), is transported into the vacuole as a GSH conjugate.
authors.affiliation: Tokyo University of Agriculture and Technology, Fuchu, Tokyo, Japan.
authors.forename: Naoko
authors.initials: N
authors.lastname: Ohkama-Ohtsu
authors.forename: Yuko
authors.initials: Y
authors.lastname: Sasaki-Sekimoto
authors.forename: Akira
authors.initials: A
authors.lastname: Oikawa
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Jikumaru
authors.forename: Shoko
authors.initials: S
authors.lastname: Shinoda
authors.forename: Eri
authors.initials: E
authors.lastname: Inoue
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Kamide
authors.forename: Tadashi
authors.initials: T
authors.lastname: Yokoyama
authors.forename: Masami Yokota
authors.initials: MY
authors.lastname: Hirai
authors.forename: Ken
authors.initials: K
authors.lastname: Shirasu
authors.forename: Yuji
authors.initials: Y
authors.lastname: Kamiya
authors.forename: David J
authors.initials: DJ
authors.lastname: Oliver
authors.forename: Kazuki
authors.initials: K
authors.lastname: Saito
mesh_terms: D017360:Arabidopsis / Q000378:metabolism*; D001483:Base Sequence; D017931:DNA Primers; D005231:Fatty Acids, Unsaturated / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D014617:Vacuoles / Q000378:metabolism*
chemical_list: D017931:DNA Primers; D005231:Fatty Acids, Unsaturated; C025999:12-oxophytodienoic acid; D005978:Glutathione