title: Regulation of cell cycle-specific gene expression in fission yeast by the Cdc14p-like phosphatase Clp1p.
abstract: Regulated gene expression makes an important contribution to cell cycle control mechanisms. In fission yeast, a group of genes is coordinately expressed during a late stage of the cell cycle (M phase and cytokinesis) that is controlled by common cis-acting promoter motifs named pombe cell cycle boxes (PCBs), which are bound by a trans-acting transcription factor complex, PCB binding factor (PBF). PBF contains at least three transcription factors, a MADS box protein Mbx1p and two forkhead transcription factors, Sep1p and Fkh2p. Here we show that the fission yeast Cdc14p-like phosphatase Clp1p (Flp1p) controls M-G1 specific gene expression through PBF. Clp1p binds in vivo both to Mbx1p, a MADS box-like transcription factor, and to the promoters of genes transcribed at this cell cycle time. Because Clp1p dephosphorylates Mbx1p in vitro, and is required for Mbx1p cell cycle-specific dephosphorylation in vivo, our observations suggest that Clp1p controls cell cycle-specific gene expression through binding to and dephosphorylating Mbx1p.
authors.affiliation: Division of Molecular and Cellular Biology, Faculty of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, UK.
authors.forename: Kyriaki
authors.initials: K
authors.lastname: Papadopoulou
authors.forename: Jun-Song
authors.initials: JS
authors.lastname: Chen
authors.forename: Emma
authors.initials: E
authors.lastname: Mead
authors.forename: Anna
authors.initials: A
authors.lastname: Feoktistova
authors.forename: Claudia
authors.initials: C
authors.lastname: Petit
authors.forename: Monica
authors.initials: M
authors.lastname: Agarwal
authors.forename: Mohhammed
authors.initials: M
authors.lastname: Jamal
authors.forename: Asrar
authors.initials: A
authors.lastname: Malik
authors.forename: Adonis
authors.initials: A
authors.lastname: Spanos
authors.forename: Steven G
authors.initials: SG
authors.lastname: Sedgwick
authors.forename: Jim
authors.initials: J
authors.lastname: Karagiannis
authors.forename: Mohan K
authors.initials: MK
authors.lastname: Balasubramanian
authors.forename: Kathleen L
authors.initials: KL
authors.lastname: Gould
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: McInerny
mesh_terms: D002453:Cell Cycle / Q000235:genetics*; D018797:Cell Cycle Proteins / Q000378:metabolism*; D016193:G1 Phase / Q000235:genetics; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal / Q000235:genetics; D008938:Mitosis / Q000235:genetics; D008957:Models, Genetic; D010749:Phosphoprotein Phosphatases / Q000378:metabolism*; D010766:Phosphorylation; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding; D017027:Protein Tyrosine Phosphatases / Q000378:metabolism*; D012568:Schizosaccharomyces / Q000166:cytology* / Q000201:enzymology / Q000235:genetics*; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D018797:Cell Cycle Proteins; D029702:Schizosaccharomyces pombe Proteins; C503885:CDC14 protein, S pombe; D010749:Phosphoprotein Phosphatases; C439269:Clp1 protein, S. pombe; D017027:Protein Tyrosine Phosphatases