title: Screening for epigenetic target genes that enhance reprogramming using lentiviral-delivered shRNA.
abstract: Small molecules will need to be identified and/or developed that target protein classes limiting reprogramming efficiency. A specific class of proteins includes epigenetic regulators that silence, or minimize expression, of pluripotency genes in differentiated cells. To better understand the role of specific epigenetic modulators in reprogramming, we have used shRNA delivered by lentivirus to assess the significance of individual epi-proteins in reprogramming pluripotent gene expression.
authors.affiliation: Nuclear Reprogramming and Epigenetics Laboratory, NuPotential, Inc., Louisiana Emerging Technology Center, Baton Rouge, LA, USA.
authors.forename: Jong S
authors.initials: JS
authors.lastname: Rim
authors.forename: Karen
authors.initials: K
authors.lastname: Strickler
authors.forename: Christian
authors.initials: C
authors.lastname: Barnes
authors.forename: Lettie
authors.initials: L
authors.lastname: Harkins
authors.forename: Jaroslaw
authors.initials: J
authors.lastname: Staszkiewicz
authors.forename: Rachel
authors.initials: R
authors.lastname: Power
authors.forename: Kenneth J
authors.initials: KJ
authors.lastname: Eilertsen
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000378:metabolism; D002478:Cells, Cultured; D065150:Cellular Reprogramming / Q000235:genetics*; D003114:Colony-Forming Units Assay; D018076:DNA, Complementary / Q000096:biosynthesis; D020405:Dermis / Q000166:cytology; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism; D044127:Epigenesis, Genetic; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D005820:Genetic Testing / Q000379:methods*; D006801:Humans; D007150:Immunohistochemistry; D057026:Induced Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism; D016086:Lentivirus / Q000235:genetics*; D008168:Lung / Q000166:cytology; D032342:Muscle Cells / Q000166:cytology / Q000378:metabolism; D018482:Muscle, Skeletal / Q000166:cytology; D034741:RNA, Small Interfering / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D018076:DNA, Complementary; D034741:RNA, Small Interfering