title: Viral transduction of adipose-derived stem cells.
abstract: Increasing numbers of regenerative approaches now involve use of adult stem cells, like the bone marrow MSC or the adipose-derived ASC. With their ease of in vitro manipulation and successful tissue integration in vivo, the ASC makes an attractive candidate for gene delivery in vivo using viral-based gene therapy strategies. As such, this chapter describes methods for the transduction of human ASCs with two popular types of recombinant viruses: adenovirus and lentivirus.
authors.affiliation: Regenerative Bioengineering and Repair Laboratory, Division of Plastic Surgery, Department of Surgery, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA.
authors.forename: Patricia A
authors.initials: PA
authors.lastname: Zuk
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D000273:Adipose Tissue / Q000166:cytology*; D006801:Humans; D016086:Lentivirus / Q000235:genetics*; D011995:Recombination, Genetic; D013234:Stem Cells / Q000378:metabolism* / Q000821:virology*; D014161:Transduction, Genetic / Q000379:methods*