title: Automated sample preparation for regulated bioanalysis: an integrated multiple assay extraction platform using robotic liquid handling.
abstract: BACKGROUND
  A novel approach for regulated bioanalytical sample preparation has been developed to combine multiple types of extraction techniques into one integrated and automated sample-preparation suite that pairs a graphical user interface with the Hamilton Microlab(®) STAR robotic liquid handler.
  
  
  RESULTS
  The multi-assay sample-preparation suite is composed of three bioanalytical extraction techniques: protein precipitation, solid-phase extraction and liquid-liquid extraction. Validation data provided highly reproducible and robust results for each respective automated extraction technique.
  
  
  CONCLUSIONS
  The user-friendly graphical user interface and modular method design provide a flexible and versatile approach for routine bioanalytical sample-preparation and is the first fully integrated multiple assay sample-preparation suite for regulated bioanalysis.
authors.affiliation: Pharmacokinetics, Dynamics & Metabolism, Pfizer Global Research and Development, Pfizer Inc., Groton, CT 06340, USA. joseph.tweed@pfizer.com
authors.forename: Joseph A
authors.initials: JA
authors.lastname: Tweed
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Gu
authors.forename: Hui
authors.initials: H
authors.lastname: Xu
authors.forename: Guodong
authors.initials: G
authors.lastname: Zhang
authors.forename: Parya
authors.initials: P
authors.lastname: Nouri
authors.forename: Ming
authors.initials: M
authors.lastname: Li
authors.forename: Rick
authors.initials: R
authors.lastname: Steenwyk
mesh_terms: D053000:Analytic Sample Preparation Methods / Q000295:instrumentation / Q000379:methods*; D001331:Automation; D005591:Chemical Fractionation / Q000295:instrumentation / Q000379:methods*; D011232:Chemical Precipitation; D003196:Computer Graphics; D055808:Drug Discovery; D006801:Humans; D004364:Pharmaceutical Preparations / Q000032:analysis / Q000302:isolation & purification; D011506:Proteins / Q000737:chemistry; D012371:Robotics; D052616:Solid Phase Extraction; D018511:Systems Integration; D014584:User-Computer Interface
chemical_list: D004364:Pharmaceutical Preparations; D011506:Proteins