title: Protein kinase affinity reagents based on a 5-aminoindazole scaffold.
abstract: Affinity reagents that target protein kinases are powerful tools for signal transduction research. Here, we describe a general set of kinase ligands based on a 5-aminoindazole scaffold. This scaffold can readily be derivatized with diverse binding elements and immobilized analogs allow selective enrichment of protein kinases from complex mixtures.
authors.affiliation: Department of Chemistry, Box 351700, University of Washington, Seattle, WA 98195-1700, United States.
authors.forename: Ratika
authors.initials: R
authors.lastname: Krishnamurty
authors.forename: Amanda M
authors.initials: AM
authors.lastname: Brock
authors.forename: Dustin J
authors.initials: DJ
authors.lastname: Maly
mesh_terms: D002851:Chromatography, High Pressure Liquid; D007191:Indazoles / Q000138:chemical synthesis / Q000737:chemistry*; D011485:Protein Binding; D011494:Protein Kinases / Q000737:chemistry* / Q000378:metabolism; D015398:Signal Transduction; D013329:Structure-Activity Relationship
chemical_list: D007191:Indazoles; C014974:5-aminoindazole; D011494:Protein Kinases