title: Multiplexed evaluation of capture agent binding kinetics using arrays of silicon photonic microring resonators.
abstract: High affinity capture agents recognizing biomolecular targets are essential in the performance of many proteomic detection methods. Herein, we report the application of a label-free silicon photonic biomolecular analysis platform for simultaneously determining kinetic association and dissociation constants for two representative protein capture agents: a thrombin-binding DNA aptamer and an anti-thrombin monoclonal antibody. The scalability and inherent multiplexing capability of the technology make it an attractive platform for simultaneously evaluating the binding characteristics of multiple capture agents recognizing the same target antigen, and thus a tool complementary to emerging high-throughput capture agent generation strategies.
authors.affiliation: Department of Chemistry, University of Illinois at Urbana-Champaign, 600 South Mathews Avenue, Urbana, Illinois 61801, USA.
authors.forename: Ji-Yeon
authors.initials: JY
authors.lastname: Byeon
authors.forename: Ryan C
authors.initials: RC
authors.lastname: Bailey
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D052157:Aptamers, Nucleotide / Q000378:metabolism*; D015374:Biosensing Techniques / Q000295:instrumentation*; D004867:Equipment Design; D006801:Humans; D057230:Limit of Detection; D055095:Optics and Photonics / Q000295:instrumentation*; D011485:Protein Binding; D012825:Silicon / Q000737:chemistry*; D013917:Thrombin / Q000032:analysis* / Q000276:immunology / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D052157:Aptamers, Nucleotide; C081676:thrombin aptamer; D013917:Thrombin; D012825:Silicon