title: Role of microRNA-21 and effect on PTEN in Kazakh's esophageal squamous cell carcinoma.
abstract: The aim of this study was investigate the role of microRNA-21 (miR-21) and its regulation on phosphatase and tensin homolog deleted from chromosome-10 (PTEN) in Kazakh's esophageal squamous cell carcinoma (ESCC). MiR-21 expressions were investigated in esophageal cancer cell line Eca109, and 18 pairs of Kazakh's ESCC and adjacent normal tissues by real-time quantitative PCR (qRT-PCR). To evaluate the role of miR-21 and PTEN, cell proliferations were analyzed with miR-21 mimics or their inhibitor-transfected cells. Moreover, the expressions of PTEN were performed by Western blotting. In Eca109, when transfected with miR-21 mimics, accumulation of miR-21 was obviously increased and expression of PTEN protein was decreased to be approximately 40%, which resulted in the promotion of cell proliferation. However, when transfected with miR-21 inhibitor, expression of miR-21 was declined and PTEN protein was overexpressed to be approximately 79%, which resulted in the suppression of cell proliferation. Both of them had no effect on the level of PTEN mRNA. Compared with adjacent normal tissues, miR-21 expression was significantly higher in tumor (P < 0.05). Specifically, patients with cancer cell invasion deep into esophageal serosa showed significantly higher expression of miR-21. Protein expression of PTEN was significantly lower in tumor compared with normal tissues (P < 0.05); however, mRNA expression of PTEN had no obvious significance between them. Furthermore, there was a significantly inverse correlation between miR-21 expression and PTEN protein levels (p < 0.05). The author concluded that MiR-21 was overexpressed in vitro and ESCC, and promoted the cell proliferation, might target PTEN at post-transcriptional level, and regulated the cancer invasion in Kazakh's ESCC.
authors.affiliation: Medical Research Center, The First Affiliated Hospital, Xinjiang Medical University, Urumqi, 830054 Xinjiang Uygur Autonomous Region, People's Republic of China.
authors.forename: Wen-Jing
authors.initials: WJ
authors.lastname: Ma
authors.forename: Guo-Dong
authors.initials: GD
authors.lastname: Lv
authors.forename: Aerziguli
authors.initials: A
authors.lastname: Tuersun
authors.forename: Qing
authors.initials: Q
authors.lastname: Liu
authors.forename: Hui
authors.initials: H
authors.lastname: Liu
authors.forename: Shu-Tao
authors.initials: ST
authors.lastname: Zheng
authors.forename: Cong-Gai
authors.initials: CG
authors.lastname: Huang
authors.forename: Jun-Guo
authors.initials: JG
authors.lastname: Feng
authors.forename: Xing
authors.initials: X
authors.lastname: Wang
authors.forename: Ren-Yong
authors.initials: RY
authors.lastname: Lin
authors.forename: Ilyar
authors.initials: I
authors.lastname: Sheyhidin
authors.forename: Xiao-Mei
authors.initials: XM
authors.lastname: Lu
mesh_terms: D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology / Q000503:physiopathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002681:China; D018450:Disease Progression; D004938:Esophageal Neoplasms / Q000235:genetics* / Q000473:pathology / Q000503:physiopathology; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007623:Kazakhstan; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D051059:PTEN Phosphohydrolase / Q000235:genetics / Q000378:metabolism*; D014162:Transfection
chemical_list: C524940:MIRN21 microRNA, human; D035683:MicroRNAs; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human