title: Rapid HPLC method for the simultaneous monitoring of duloxetine, venlaflaxine, fluoxetine and paroxetine in biofluids.
abstract: A simple and rapid HPLC method is developed for the determination of two serotonin-norepinephrine-reuptake inhibitors (duloxetine and venlaflaxine) and two selective serotonin-reuptake inhibitors (fluoxetine and paroxetine) in human biofluids. Separation was performed on an Inertsil ODS-3 column (250 x 4.0 mm, 5 µm) with acetonitrile-ammonium acetate (0.05 M, 41:59 v/v) at 235 nm, within 7 min. SPE on Oasis(®) HLB cartridges was applied for the isolation of analytes from biofluids. The developed methodology was validated in terms of sensitivity, linearity, accuracy, precision, stability and selectivity. Relative standard deviation was less than 10.4%. Limit of detection was 0.2-0.6 ng/µl in blood plasma and 0.1-0.8 ng/µl in urine. The method was successfully applied to biofluids from a patient under duloxetine treatment.
authors.affiliation: Laboratory of Analytical Chemistry, Department of Chemistry, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, Greece. samanidu@chem.auth.gr
authors.forename: Victoria F
authors.initials: VF
authors.lastname: Samanidou
authors.forename: Paraskevi V
authors.initials: PV
authors.lastname: Kourti
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D003511:Cyclohexanols / Q000032:analysis* / Q000097:blood / Q000652:urine; D000068736:Duloxetine Hydrochloride; D005473:Fluoxetine / Q000032:analysis* / Q000097:blood / Q000652:urine; D006801:Humans; D057230:Limit of Detection; D017374:Paroxetine / Q000032:analysis* / Q000097:blood / Q000652:urine; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D017367:Selective Serotonin Reuptake Inhibitors / Q000032:analysis* / Q000097:blood / Q000652:urine; D013876:Thiophenes / Q000032:analysis* / Q000097:blood / Q000652:urine; D000069470:Venlafaxine Hydrochloride
chemical_list: D003511:Cyclohexanols; D000068736:Duloxetine Hydrochloride; D005473:Fluoxetine; D017374:Paroxetine; D017367:Selective Serotonin Reuptake Inhibitors; D013876:Thiophenes; D000069470:Venlafaxine Hydrochloride