title: [Construction of MHV-A59 damp-heat mouse model and analysis of the relevant indices].
abstract: OBJECTIVE
  To explore the impact of inflammation, water metabolism and immune function on the establishment of a mouse model of damp-heat syndrome with MHV-A59 infection.
  
  
  METHODS
  Twenty-four mice were randomly divided into control group, virus group, damp-heat group and model group. The peripheral blood CD4(+) and CD8(+) lymphocytes were detected by flow cytometry, and the serum levels of IFN-γ and IL-4 were assayed by ELISA. The expressions of NF-κB and AQP4 in the liver and stomach were determined using immunohistochemistry.
  
  
  RESULTS
  The expression of NF-κB and CD4(+)/CD8(+) ratio in the virus and model groups were significantly higher than those in the damp-heat and control groups, while the expression of AQP4 was significantly higher in the model and damp-heat groups than in the other groups. Compared with the control group, the model group showed a significantly higher ratio of IFN-γ/IL-4.
  
  
  CONCLUSIONS
  MHV-A59 virus is the main cause of elevated NF-κB expression and CD4(+)/CD8(+)/ ratio, while damp-heat syndrome is responsible for increased AQP4 expression, and their synergistic effect results in increased IFN-γ/IL-4 ratio. The mouse model established using MHV-A59 virus and the damp-heat factors can mimic damp-heat syndrome described in traditional Chinese medicine theory.
authors.affiliation: School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515, China. joyt5786@hotmail.com
authors.forename: Zhao-hui
authors.initials: ZH
authors.lastname: Tang
authors.forename: Li-xian
authors.initials: LX
authors.lastname: Su
authors.forename: Hua-feng
authors.initials: HF
authors.lastname: Li
authors.forename: Jin-qiang
authors.initials: JQ
authors.lastname: Guo
authors.forename: Bing-de
authors.initials: BD
authors.lastname: Luo
authors.forename: Pei-zheng
authors.initials: PZ
authors.lastname: Lin
mesh_terms: D000818:Animals; D051401:Aquaporin 4 / Q000378:metabolism; D016516:CD4-CD8 Ratio; D004195:Disease Models, Animal; D006524:Hepatitis, Viral, Animal / Q000175:diagnosis* / Q000821:virology*; D007371:Interferon-gamma / Q000097:blood; D015847:Interleukin-4 / Q000097:blood; D008297:Male; D008516:Medicine, Chinese Traditional; D051379:Mice; D008807:Mice, Inbred BALB C; D006517:Murine hepatitis virus; D052002:NF-kappa B p50 Subunit / Q000378:metabolism
chemical_list: C496031:Aqp4 protein, mouse; D051401:Aquaporin 4; D052002:NF-kappa B p50 Subunit; C073701:Nfkb1 protein, mouse; D015847:Interleukin-4; D007371:Interferon-gamma