title: Transplantation of muscle-derived stem cells plus biodegradable fibrin glue restores the urethral sphincter in a pudendal nerve-transected rat model.
abstract: We investigated whether fibrin glue (FG) could promote urethral sphincter restoration in muscle-derived stem cell (MDSC)-based injection therapies in a pudendal nerve-transected (PNT) rat, which was used as a stress urinary incontinence (SUI) model. MDSCs were purified from the gastrocnemius muscles of 4-week-old inbred female SPF Wistar rats and labeled with green fluorescent protein. Animals were divided into five groups (N = 15): sham (S), PNT (D), PNT+FG injection (F), PNT+MDSC injection (M), and PNT+MDSC+FG injection (FM). Each group was subdivided into 1- and 4-week groups. One and 4 weeks after injection into the proximal urethra, leak point pressure (LPP) was measured to assess urethral resistance function. Histology and immunohistochemistry were performed 4 weeks after injection. LPP was increased significantly in FM and M animals after implantation compared to group D (P < 0.01), but was not different from group S. LPP was slightly higher in the FM group than in the M group but there was no significant difference between them at different times. Histological and immunohistochemical examination demonstrated increased numbers of surviving MDSCs (109 ± 19 vs 82 ± 11/hpf, P = 0.026), increased muscle/collagen ratio (0.40 ± 0.02 vs 0.34 ± 0.02, P = 0.044), as well as increased microvessel density (16.9 ± 0.6 vs 14.1 ± 0.4/hpf, P = 0.001) at the injection sites in FM compared to M animals. Fibrin glue may potentially improve the action of transplanted MDSCs to restore the histology and function of the urethral sphincter in a SUI rat model. Injection of MDSCs with fibrin glue may provide a novel cellular therapy method for SUI.
authors.affiliation: Fuzong Clinical College, Fujian Medical University, Fuzhou, Fujian, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Xu
authors.forename: Y F
authors.initials: YF
authors.lastname: Song
authors.forename: Z X
authors.initials: ZX
authors.lastname: Lin
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D015718:Fibrin Tissue Adhesive / Q000627:therapeutic use*; D007150:Immunohistochemistry; D018485:Muscle Fibers, Skeletal / Q000637:transplantation*; D018482:Muscle, Skeletal / Q000166:cytology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D033581:Stem Cell Transplantation / Q000379:methods*; D014550:Urinary Incontinence, Stress / Q000601:surgery*; D014841:von Willebrand Factor / Q000032:analysis
chemical_list: D015718:Fibrin Tissue Adhesive; D014841:von Willebrand Factor