title: A dual platform for selective analyte enrichment and ionization in mass spectrometry using aptamer-conjugated graphene oxide.
abstract: This study demonstrates the use of aptamer-conjugated graphene oxide as an affinity extraction and detection platform for analytes from complex biological media. We have shown that cocaine and adenosine can be selectively enriched from plasma samples and that direct mass spectrometric readouts can be obtained without a matrix and with greatly improved signal-to-noise ratios. Aptamer-conjugated graphene oxide has clear advantages in target enrichment and in generating highly efficient ionization of target molecules for mass spectrometry. These results demonstrate the utility of the approach for analysis of small molecules in real biological samples.
authors.affiliation: Center for Research at the Bio/Nano Interface, Department of Chemistry, University of Florida, Gainesville, Florida 32611-7200, USA.
authors.forename: Basri
authors.initials: B
authors.lastname: Gulbakan
authors.forename: Emir
authors.initials: E
authors.lastname: Yasun
authors.forename: M Ibrahim
authors.initials: MI
authors.lastname: Shukoor
authors.forename: Zhi
authors.initials: Z
authors.lastname: Zhu
authors.forename: Mingxu
authors.initials: M
authors.lastname: You
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Tan
authors.forename: Hernan
authors.initials: H
authors.lastname: Sanchez
authors.forename: David H
authors.initials: DH
authors.lastname: Powell
authors.forename: Hongjie
authors.initials: H
authors.lastname: Dai
authors.forename: Weihong
authors.initials: W
authors.lastname: Tan
mesh_terms: D000241:Adenosine / Q000097:blood / Q000302:isolation & purification*; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D003042:Cocaine / Q000097:blood / Q000302:isolation & purification*; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D010087:Oxides / Q000737:chemistry*; D059629:Signal-To-Noise Ratio
chemical_list: D052157:Aptamers, Nucleotide; D010087:Oxides; D006108:Graphite; D003042:Cocaine; D000241:Adenosine