title: Polycaprolactone diacrylate crosslinked biodegradable semi-interpenetrating networks of polyacrylamide and gelatin for controlled drug delivery.
abstract: A biodegradable semi-interpenetrating hydrogel network (semi-IPN) of polyacrylamide and gelatin was prepared using polycaprolactone diacrylate (mol. wt ∼ 640) as a crosslinker. The drug-polymer interaction and IPN formation were investigated by attenuated total reflectance-Fourier transform infrared (ATR-FTIR) and thermal gravimetric analysis (TGA). Scanning electron micrographs of lyophilized matrices revealed porous internal structure with varying pore sizes under equilibrium hydrated conditions, depending upon formulation composition. pH-dependent swelling and degradation was enhanced with increasing gelatin content and decreasing crosslinker concentration (Cs). Compression modulus (CM) (at 20% strain) increased significantly from 23 ± 1.4 to 75 ± 2.7 kPa (p < 0.02) with increasing Cs (from 0.5 to 2.0 mol%), while it decreased from 162 ± 6.4 to 23 ± 1.4 kPa (p < 0.05) with decreasing PAm/G ratio. Cell viability studies by MTT assay showed excellent cytocompatibility of matrices with fibroblast L929 cells. Curcumin, a hydrophobic phytochemical, was loaded by a diffusion method and its release profile was investigated in 4% w/v aqueous BSA solution at 75 rpm (at 37 ± 0.2 °C). Fitting of drug release data in the Korsmeyer-Peppas model suggested sustained release behavior up to 10 days with a combination of diffusion and erosion mechanism (0.5 < n < 1.0; M(t)/M(∞) ≤ 0.6). The newly developed porous, biodegradable and elastic semi-IPNs may serve as an ideal matrix for controlled drug release and wound healing applications. The possibilities can be explored for pharmaceutical and tissue engineering applications.
authors.affiliation: Centre for Biomedical Engineering, Indian Institute of Technology, New Delhi 110016, India.
authors.forename: Maneesh
authors.initials: M
authors.lastname: Jaiswal
authors.forename: Amit K
authors.initials: AK
authors.lastname: Dinda
authors.forename: Asheesh
authors.initials: A
authors.lastname: Gupta
authors.forename: Veena
authors.initials: V
authors.lastname: Koul
mesh_terms: D020341:Absorbable Implants; D000042:Absorption; D000179:Acrylates / Q000737:chemistry; D000180:Acrylic Resins / Q000737:chemistry*; D001826:Body Fluids / Q000737:chemistry*; D003432:Cross-Linking Reagents / Q000737:chemistry; D003474:Curcumin / Q000008:administration & dosage / Q000737:chemistry*; D003692:Delayed-Action Preparations / Q000138:chemical synthesis*; D004058:Diffusion; D005780:Gelatin / Q000737:chemistry*; D008422:Materials Testing; D011091:Polyesters / Q000737:chemistry*; D016062:Porosity; D000099166:Polyacrylamides
chemical_list: D000179:Acrylates; D000180:Acrylic Resins; D003432:Cross-Linking Reagents; D003474:Curcumin; D003692:Delayed-Action Preparations; D005780:Gelatin; D011091:Polyesters; D000099166:Polyacrylamides; C016240:polycaprolactone