title: Treatment of high fat diet induced type 2 diabetes in C57BL/6J mice by two medicinal plants used in traditional treatment of diabetes in the east of Algeria.
abstract: OBJECTIVE
  Hydro-alcoholic extracts of Centaurium erythraea Rafn (CE), Gentianaceae and Artemisia herba-alba Asso (AHA), Asteraceae, medicinal plants used in traditional treatment of diabetes in north-eastern Algeria, were tested in established type 2 diabetes induced with a standardized high fat diet (HFD) in mice.
  
  
  METHODS
  After confirmation of diabetes (17th week), plant extracts were administered orally by gavage at a dose of 2 g/kg daily for 18 weeks to male C57BL/6J mice fed HFD. Animals were weighed, food intake and plasma glucose measured weekly, insulin and lipid profile at study end.
  
  
  RESULTS
  At 35 weeks, groups treated with AHA or CE vs. HFD control had a significant reduction in mean (±SD) fasting blood glucose concentrations (143.8±23.9 and 139.5±14.2 vs. 229.0±20.8 mg/dL, p<0.05, respectively), triglyceride (18.9±11.1 and 16.0±6.5 vs. 62.8±18.3 mg/dL, p<0.05), total cholesterol (1.2±0.1 and 1.2±0.3 vs. 1.8±1.1 g/L, p<0.05) and serum insulin concentrations (1.7±0.7 and 0.9±0.7 vs. 3.3±14.3 ng/mL, p<0.05). Plant extracts also markedly reduced insulin resistance as compared to HFD controls (AHA: 15.6±9.1, CE: 9.0±7.7 vs. HFD control 38.5±30.3, p<0.05). The plant extracts decreased calorie intake and had little effect on body weight or HDL-cholesterol.
  
  
  CONCLUSIONS
  AHA has already been shown to have a antihyperglycaemic and antihyperlipidemic effect but this is the first demonstration of an effect of AHA and CE on established HFD-induced diabetes.
authors.affiliation: Département de Pharmacologie, Université Victor Segalen Bordeaux 2, Bordeaux, France.
authors.forename: Nawel
authors.initials: N
authors.lastname: Hamza
authors.forename: Bénédicte
authors.initials: B
authors.lastname: Berke
authors.forename: Catherine
authors.initials: C
authors.lastname: Cheze
authors.forename: Raphaële
authors.initials: R
authors.lastname: Le Garrec
authors.forename: Régis
authors.initials: R
authors.lastname: Lassalle
authors.forename: Abdel-Nacer
authors.initials: AN
authors.lastname: Agli
authors.forename: Philip
authors.initials: P
authors.lastname: Robinson
authors.forename: Henri
authors.initials: H
authors.lastname: Gin
authors.forename: Nicholas
authors.initials: N
authors.lastname: Moore
mesh_terms: D000462:Algeria; D000818:Animals; D019306:Artemisia; D001786:Blood Glucose / Q000378:metabolism; D029966:Centaurium; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000188:drug therapy* / Q000209:etiology; D004041:Dietary Fats / Q000008:administration & dosage; D020834:Ethnopharmacology; D007004:Hypoglycemic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D007328:Insulin / Q000097:blood; D008055:Lipids / Q000097:blood; D008297:Male; D016488:Medicine, African Traditional; D051379:Mice; D008810:Mice, Inbred C57BL; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology; D010946:Plants, Medicinal
chemical_list: D001786:Blood Glucose; D004041:Dietary Fats; D007004:Hypoglycemic Agents; D007328:Insulin; D008055:Lipids; D010936:Plant Extracts