title: Hepatoprotective and antioxidant effects of Hibiscus sabdariffa extract against carbon tetrachloride-induced hepatocyte damage in Cyprinus carpio.
abstract: The present study aims to evaluate the hepatoprotective and antioxidant effects of Hibiscus sabdariffa extract on the carbon tetrachloride (CCl(4))-induced hepatocyte damage in fish and provide evidence as to whether it can be potentially used as a medicine for liver diseases in aquaculture. H. sabdariffa extract (100, 200, and 400 μg/mL) was added to the carp primary hepatocyte culture before (pre-treatment), after (post-treatment), and both before and after (pre- and post-treatment) the incubation of the hepatocytes with CCl(4). CCl(4) at 8 mM in the culture medium produced significantly elevated levels of lactate dehydrogenase (LDH), glutamate oxalate transaminase (GOT), glutamate pyruvate transaminase (GPT), and malondialdehyde (MDA) and significantly reduced levels of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px). Pre-treatment and pre- and post-treatment of the hepatocytes with H. sabdariffa extract significantly reduced the elevated levels of LDH, GOT, GPT, and MDA and increased the reduced activities of SOD and GSH-Px in a dose-dependent manner; post-treatment did not show any protective effect. The results suggest that H. sabdariffa extract can be potentially used for preventing rather than curing liver diseases in fish.
authors.affiliation: Key Open Laboratory for Genetic Breeding of Aquatic Animals and Aquaculture Biology, Ministry of Agriculture, Freshwater Fisheries Research Center of Chinese Academy of Fishery Science, No. 9 East Shanshui Rd., Wuxi, China. yingj@ffrc.cn
authors.forename: Guojun
authors.initials: G
authors.lastname: Yin
authors.forename: Liping
authors.initials: L
authors.lastname: Cao
authors.forename: Pao
authors.initials: P
authors.lastname: Xu
authors.forename: Galina
authors.initials: G
authors.lastname: Jeney
authors.forename: Miki
authors.initials: M
authors.lastname: Nakao
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000032:analysis / Q000494:pharmacology*; D017756:Aquaculture / Q000379:methods; D002251:Carbon Tetrachloride / Q000633:toxicity*; D002347:Carps; D004305:Dose-Response Relationship, Drug; D005393:Fish Diseases / Q000378:metabolism / Q000517:prevention & control*; D005979:Glutathione Peroxidase / Q000378:metabolism; D022781:Hepatocytes / Q000187:drug effects*; D031584:Hibiscus / Q000737:chemistry*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008107:Liver Diseases / Q000378:metabolism / Q000517:prevention & control / Q000662:veterinary*; D008315:Malondialdehyde / Q000378:metabolism; D008517:Phytotherapy / Q000379:methods; D010936:Plant Extracts / Q000032:analysis / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism; D000637:Transaminases / Q000378:metabolism
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D008315:Malondialdehyde; D002251:Carbon Tetrachloride; D007770:L-Lactate Dehydrogenase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D000637:Transaminases