title: Synthesis and crystal structure of two new dinuclear cobalt(II) complexes interaction with HeLa cells.
abstract: Two novel dinuclear complexes [Co(2)(L)(2)(phen)(2)·2H(2)O](n) (1), [Co(2)(L)(2)(bipy)(2)](n) (2) (where phen=1,10-phenanthroline, bipy=2,2'-bipyridine, and L=biphenyl-2,4'-dicarboxylic acid) have been synthesized and characterized using IR, (1)H NMR, element analysis and single-crystal X-ray diffractometry. The binding of the complexes with HC-DNA (HeLa cells DNA, which was extracted by ourselves) was investigated by fluorescence spectrum. The experimental results show that the two complexes have the ability to bind with extracted HC-DNA. Gel electrophoresis assay demonstrates the ability of the complexes to cleave the extracted HC-DNA. The complexes exhibit a higher cytotoxicity against tumor cells as against normal cells in vitro. Further more, the apoptotic tests indicate that the complexes have an apoptotic effect on HeLa cells.
authors.affiliation: Laboratory of Coordination Chemistry, Shenyang University of Chemical Technology, Shenyang Street, Shenyang 110142, People's Republic of China. ejgao@yahoo.com.cn
authors.forename: En-Jun
authors.initials: EJ
authors.lastname: Gao
authors.forename: Xia-Nan
authors.initials: XN
authors.lastname: Gao
authors.forename: Feng
authors.initials: F
authors.lastname: Guan
authors.forename: Ming-Chang
authors.initials: MC
authors.lastname: Zhu
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.forename: Ying-Xing
authors.initials: YX
authors.lastname: Zhang
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.forename: Zheng
authors.initials: Z
authors.lastname: Wen
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.forename: Qi
authors.initials: Q
authors.lastname: Liang
mesh_terms: D000970:Antineoplastic Agents / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D002264:Carboxylic Acids / Q000737:chemistry; D002470:Cell Survival / Q000187:drug effects; D003035:Cobalt / Q000737:chemistry*; D018360:Crystallography, X-Ray; D004247:DNA / Q000302:isolation & purification / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D008024:Ligands; D008958:Models, Molecular; D008968:Molecular Conformation; D009942:Organometallic Compounds / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D013050:Spectrometry, Fluorescence
chemical_list: D000970:Antineoplastic Agents; D002264:Carboxylic Acids; D008024:Ligands; D009942:Organometallic Compounds; D003035:Cobalt; D004247:DNA