title: Comparison of in vitro maturation media of immature oocytes: the effectiveness of blastocyst culture media.
abstract: OBJECTIVE
  To compare three different in vitro maturation (IVM) media for immature oocytes.
  
  
  METHODS
  Experimental study.
  
  
  METHODS
  In vitro fertilization laboratory.
  
  
  METHODS
  BDF1 female and male mice.
  
  
  METHODS
  Retrieval and maturation of cumulus-enclosed germinal vesicle-stage oocytes according to one of three protocols: group A, conventional IVM medium; group B, blastocyst culture medium; and group C, tissue culture medium (TCM) 199.
  
  
  METHODS
  Maturation, fertilization, and developmental rates of immature oocytes.
  
  
  RESULTS
  A total of 653 immature oocytes were cultured in vitro and then analyzed. No difference was found in maturation rates and fertilization rates in comparing groups A and B. However, the IVM rates were statistically significantly increased in groups A and B compared with group C. No difference was found in fertilization rates between media, but the developmental competency to blastocyst stage was statistically significantly higher in group B compared with group C.
  
  
  CONCLUSIONS
  The developmental competency of immature oocytes did not differ between conventional IVM medium and blastocyst culture medium, but TCM-199 was found to be unsuitable. Evidence from mice as test subjects suggests that both conventional IVM medium and blastocyst culture medium are suitable for IVM, and that blastocyst culture medium may be a good choice for conventional IVM of immature oocytes.
authors.affiliation: Department of Obstetrics and Gynecology, Ajou University School of Medicine, Suwon, South Korea.
authors.forename: Miran
authors.initials: M
authors.lastname: Kim
authors.forename: Sun Jung
authors.initials: SJ
authors.lastname: Hong
authors.forename: Jae Hoon
authors.initials: JH
authors.lastname: Lee
authors.forename: Churl K
authors.initials: CK
authors.lastname: Min
authors.forename: Kyung Joo
authors.initials: KJ
authors.lastname: Hwang
authors.forename: Rae Woong
authors.initials: RW
authors.lastname: Park
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002970:Cleavage Stage, Ovum / Q000187:drug effects / Q000502:physiology; D003470:Culture Media / Q000494:pharmacology*; D004526:Efficiency; D046149:Embryo Culture Techniques / Q000379:methods*; D005260:Female; D005306:Fertilization / Q000187:drug effects / Q000502:physiology; D008297:Male; D051379:Mice; D054315:Oocyte Retrieval / Q000379:methods; D009865:Oocytes / Q000166:cytology* / Q000187:drug effects* / Q000502:physiology; D009866:Oogenesis / Q000187:drug effects*; D016896:Treatment Outcome
chemical_list: D003470:Culture Media