title: Adenovirus-mediated gene transfer.
abstract: Recombinant adenoviruses are attractive vectors for short-term expression in mouse liver and primary cell lines. Various versatile vector systems have been developed which can be used for the reliable production of recombinant adenoviruses. This protocol describes the entire process for the production of recombinant adenoviruses using the AdEasy system. This protocol will give a practical step-by-step description from the cloning of the gene of interest until the in vivo administration in mice. The entire process will take about 8 weeks to complete.
authors.affiliation: Department of Human Genetics, Leiden University Medical Center, Leiden, The Netherlands.
authors.forename: Ko Willems
authors.initials: KW
authors.lastname: van Dijk
authors.forename: Kyriakos E
authors.initials: KE
authors.lastname: Kypreos
authors.forename: Frits J
authors.initials: FJ
authors.lastname: Fallaux
authors.forename: Jurre
authors.initials: J
authors.lastname: Hageman
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D002478:Cells, Cultured; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000235:genetics*; D008099:Liver / Q000378:metabolism; D051379:Mice; D008957:Models, Genetic; D011995:Recombination, Genetic / Q000235:genetics; D014161:Transduction, Genetic