title: Towards monitoring real-time cellular response using an integrated microfluidics-matrix assisted laser desorption ionisation/nanoelectrospray ionisation-ion mobility-mass spectrometry platform.
abstract: The combination of microfluidic cell trapping devices with ion mobility-mass spectrometry offers the potential for elucidating in real time the dynamic responses of small populations of cells to paracrine signals, changes in metabolite levels and delivery of drugs and toxins. Preliminary experiments examining peptides in methanol and recording the interactions of yeast and Jurkat cells with their superfusate have identified instrumental set-up and control parameters and online desalting procedures. Numerous initial experiments demonstrate and validate this new instrumental platform. Future outlooks and potential applications are addressed, specifically how this instrumentation may be used for fully automated systems biology studies of the significantly interdependent, dynamic internal workings of cellular metabolic and signalling pathways.
authors.affiliation: Vanderbilt University, Department of Chemistry, Nashville, TN, USA.
authors.forename: J R
authors.initials: JR
authors.lastname: Enders
authors.forename: C C
authors.initials: CC
authors.lastname: Marasco
authors.forename: A
authors.initials: A
authors.lastname: Kole
authors.forename: B
authors.initials: B
authors.lastname: Nguyen
authors.forename: S
authors.initials: S
authors.lastname: Sevugarajan
authors.forename: K T
authors.initials: KT
authors.lastname: Seale
authors.forename: J P
authors.initials: JP
authors.lastname: Wikswo
authors.forename: J A
authors.initials: JA
authors.lastname: McLean
mesh_terms: D003585:Cell Biology / Q000295:instrumentation*; D002468:Cell Physiological Phenomena; D002469:Cell Separation / Q000295:instrumentation; D002477:Cells / Q000737:chemistry / Q000166:cytology; D006801:Humans; D019169:Jurkat Cells; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D012441:Saccharomyces cerevisiae; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D049490:Systems Biology / Q000379:methods