title: Differential occurrence of oxidative burst and antioxidative mechanism in compatible and incompatible interactions of Solanum lycopersicum and Ralstonia solanacearum.
abstract: Striking increase in reactive oxygen species (ROS) such as hydrogen peroxide (H(2)O(2)) has been demonstrated to occur in plants in response to pathogen attack. The aim of this study was to investigate the biochemical aspects of ROS generation, antioxidative mechanism and cell wall reinforcement as responses of tomato cultivars Arka Meghali (AM; susceptible) and BT-10 (BT; resistant) against Ralstonia solanacearum (Ralsol). While the oxidative burst was characterized by a single phase ROS increase in AM, there was a clear bi-phasic ROS generation in BT. The first significant increase of H(2)O(2) production was noticed at 12 h post-inoculation (hpi) followed by a sharp increase in H(2)O(2) generation after 36 hpi. Lipid peroxidation was more in roots of AM than that of BT after pathogen inoculation. Superoxide dismutase and catalase activities were continuously at very high level in Ralsol-inoculated BT plants, whereas activities of the enzymes were observed to decrease at later stage in Ralsol-inoculated AM plants. Guaiacol peroxidase activity was high in Ralsol-inoculated roots of both cultivars, but BT recorded much higher activity than AM. Higher activity of ascorbate peroxidase in inoculated BT might be an indication of better scavenging activity of the enzyme. Total phenolic content and lignin deposition were significantly higher in Ralsol-inoculated BT compared to inoculated AM. Our results indicate that increased level of ROS production coupled with more efficient antioxidative system, lower rate of lipid peroxidation and high lignin deposition in cell wall may contribute to the resistance of tomato plants to Ralsol.
authors.affiliation: Plant Pathology Laboratory, Central Horticultural Experiment Station (ICAR), Aiginia, Bhubaneswar 751019, India.
authors.forename: Sudhamoy
authors.initials: S
authors.lastname: Mandal
authors.forename: Rupa Kumar
authors.initials: RK
authors.lastname: Das
authors.forename: Sanjeev
authors.initials: S
authors.lastname: Mishra
mesh_terms: D000975:Antioxidants / Q000378:metabolism; D060387:Ascorbate Peroxidases; D002374:Catalase / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D008031:Lignin / Q000378:metabolism; D015227:Lipid Peroxidation / Q000502:physiology; D018551:Solanum lycopersicum / Q000201:enzymology / Q000378:metabolism*; D009195:Peroxidase / Q000378:metabolism; D010544:Peroxidases / Q000378:metabolism; D019800:Phenol / Q000378:metabolism; D043368:Ralstonia solanacearum / Q000201:enzymology / Q000378:metabolism*; D016897:Respiratory Burst / Q000502:physiology; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D019800:Phenol; D008031:Lignin; D006861:Hydrogen Peroxide; D010544:Peroxidases; C121048:guaiacol peroxidase; D060387:Ascorbate Peroxidases; D002374:Catalase; D009195:Peroxidase; D013482:Superoxide Dismutase