title: Nitric oxide-dependent pigment migration induced by ultraviolet radiation in retinal pigment cells of the crab Neohelice granulata.
abstract: The purpose of this study was to verify the occurrence of pigment dispersion in retinal pigment cells exposed to UVA and UVB radiation, and to investigate the possible participation of a nitric oxide (NO) pathway. Retinal pigment cells from Neohelice granulata were obtained by cellular dissociation. Cells were analyzed for 30 min in the dark (control) and then exposed to 1.1 and 3.3 J cm(-2) UVA, 0.07 and 0.9 J cm(-2) UVB, 20 nmβ-PDH (pigment dispersing hormone) or 10 μm SIN-1 (NO donor). Histological analyses were performed to verify the UV effect in vivo. Cultured cells were exposed to 250 μm L-NAME (NO synthase blocker) and afterwards were treated with UVA, UVB or β-PDH. The retinal cells in culture displayed significant pigment dispersion in response to UVA, UVB and β-PDH. The same responses to UVA and UVB were observed in vivo. SIN-1 did not induce pigment dispersion in the cell cultures. L-NAME significantly decreased the pigment dispersion induced by UVA and UVB but not by β-PDH. All retinal cells showed an immunopositive reaction against neuronal nitric oxide synthases. Therefore, UVA and UVB radiation are capable of inducing pigment dispersion in retinal pigment cells of Neohelice granulata and this dispersion may be nitric oxide synthase dependent.
authors.affiliation: Programa de Pós-graduação em Ciências Fisiológicas, Fisiologia Animal Comparada, FURG, Instituto de Ciências Biológicas, Rio Grande, Brazil.
authors.forename: Daza de Moraes Vaz Batista
authors.initials: Dde M
authors.lastname: Filgueira
authors.forename: Laís Pereira
authors.initials: LP
authors.lastname: Guterres
authors.forename: Ana Paula de Souza
authors.initials: AP
authors.lastname: Votto
authors.forename: Marcelo Alves
authors.initials: MA
authors.lastname: Vargas
authors.forename: Robert Tew
authors.initials: RT
authors.lastname: Boyle
authors.forename: Gilma Santos
authors.initials: GS
authors.lastname: Trindade
authors.forename: Luiz Eduardo Maia
authors.initials: LE
authors.lastname: Nery
mesh_terms: D000818:Animals; D003386:Brachyura / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects*; D066298:In Vitro Techniques; D008981:Molsidomine / Q000031:analogs & derivatives / Q000494:pharmacology; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009569:Nitric Oxide / Q000378:metabolism; D020030:Nitric Oxide Donors / Q000494:pharmacology; D010455:Peptides / Q000494:pharmacology; D017956:Photoreceptor Cells, Invertebrate / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D012168:Retinal Pigments / Q000378:metabolism* / Q000528:radiation effects*; D014466:Ultraviolet Rays
chemical_list: D020030:Nitric Oxide Donors; D010455:Peptides; D012168:Retinal Pigments; D009569:Nitric Oxide; C002385:linsidomine; C027714:melanophore-dispersing hormone; D008981:Molsidomine; D019331:NG-Nitroarginine Methyl Ester