title: Chemiluminescent enzyme immunoassays: a review of bioanalytical applications.
abstract: This review gives an overview of the most recent and innovative developments in the field of chemiluminescent immunoassays through carefully selected examples. First, assays using microtiter plates for high-throughput or multiplexed assays aiming to achieve more complex assays through the multiplication of parameters per wells will be described. Systems will then be presented that have been recently developed, motivated by integration and miniaturization of existing immunoassays in more complex experimental setups. Finally, enhanced-performance chemiluminescent biochips, based on chemiluminescent reaction intensification, will be introduced.
authors.affiliation: Laboratoire de Génie Enzymatique et Biomoléculaire, Institut de Chimie et Biochimie Moléculaires et Supramoléculaires, Université Lyon 1 - CNRS 5246 ICBMS, Bâtiment CPE 43, Bd du 11 Novembre 1918, 69622 Villeurbanne, Cedex, France.
authors.forename: Christophe A
authors.initials: CA
authors.lastname: Marquette
authors.forename: Loïc J
authors.initials: LJ
authors.lastname: Blum
mesh_terms: D000818:Animals; D000906:Antibodies / Q000032:analysis / Q000378:metabolism; D006735:Horseradish Peroxidase / Q000032:analysis / Q000378:metabolism; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods* / Q000639:trends; D056656:Lab-On-A-Chip Devices; D008163:Luminescent Measurements / Q000295:instrumentation / Q000379:methods* / Q000639:trends; D046208:Microchip Analytical Procedures / Q000379:methods / Q000639:trends; D008904:Miniaturization
chemical_list: D000906:Antibodies; D006735:Horseradish Peroxidase