title: The complete genome sequence of a Passion fruit woodiness virus isolate from Australia determined using deep sequencing, and its relationship to other potyviruses.
abstract: The complete genome sequence (9,858 nucleotides) of the Passion fruit woodiness virus isolate MU-2 was determined using Illumina sequencing. The large open reading frame (ORF) encodes a polyprotein containing 3,086 amino acids, with an AUG start codon and UAA stop codon. The polyprotein yielded 11 proteins (P1, HC-Pro, P3, PIPO, 6K1, CI, 6K2, NIa-VPg, NIa-Pro, NIb and CP). Putative cleavage sites between them were identified by sequence comparison to those of other known potyviruses. Accuracy of the genome sequence information was provided by 42-1691-fold sequence coverage, and viral RNA accounted for 7.38% of total polyadenylated RNA from the host plant.
authors.affiliation: Plant Virus Section, Western Australian State Agricultural Biotechnology Centre, Murdoch University, Perth, WA 6150, Australia. s.wylie@murdoch.edu.au
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Wylie
authors.forename: Michael G K
authors.initials: MG
authors.lastname: Jones
mesh_terms: D001315:Australia; D016000:Cluster Analysis; D016679:Genome, Viral; D059014:High-Throughput Nucleotide Sequencing; D008969:Molecular Sequence Data; D010802:Phylogeny; D020815:Polyproteins / Q000235:genetics; D017800:Potyvirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D012367:RNA, Viral / Q000235:genetics*; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000235:genetics
chemical_list: D020815:Polyproteins; D012367:RNA, Viral; D014764:Viral Proteins