title: Impact of indium-111 oxine labelling on viability of human mesenchymal stem cells in vitro, and 3D cell-tracking using SPECT/CT in vivo.
abstract: OBJECTIVE
  This study investigates the effects of (111)In-oxine incorporation on human mesenchymal stem cells' (hMSC) biology and viability, and the applicability of (111)In-oxine for single-photon emission computed tomography/X-ray computed tomography (SPECT/CT) monitoring of hMSC in vivo.
  
  
  METHODS
  HMSC were labelled with 10 Bq/cell. Cellular retention of radioactivity, cell survival, and migration were evaluated over 48 h. Metabolic activity was assessed over 14 days and the hMSC's stem cell character was evaluated. Serial SPECT/CT was performed after intra-osseous injection to athymic rats over 48 h.
  
  
  RESULTS
  Labelling efficiency was 25%, with 61% of incorporated (111)In remaining in the hMSC at 48 h. The radiolabelling was without effect on cell viability, stem cell character, and plasticity, whereas metabolic activity and migration were significantly reduced. Grafted cells could be imaged in situ with SPECT/CT.
  
  
  CONCLUSIONS
  (111)In-oxine labelling moderately impaired hMSC's functional integrity while preserving their stem cell character. Combined SPECT/CT imaging of (111)In-oxine-labelled hMSC opens the possibility for non-invasive sequential monitoring of therapeutic stem cells.
authors.affiliation: Department of Nuclear Medicine, University of Munich (LMU), Munich, Germany. franz.gildehaus@med.uni-muenchen.de
authors.forename: Franz Josef
authors.initials: FJ
authors.lastname: Gildehaus
authors.forename: Florian
authors.initials: F
authors.lastname: Haasters
authors.forename: Inga
authors.initials: I
authors.lastname: Drosse
authors.forename: Erika
authors.initials: E
authors.lastname: Wagner
authors.forename: Christian
authors.initials: C
authors.lastname: Zach
authors.forename: Wolf
authors.initials: W
authors.lastname: Mutschler
authors.forename: Paul
authors.initials: P
authors.lastname: Cumming
authors.forename: Peter
authors.initials: P
authors.lastname: Bartenstein
authors.forename: Matthias
authors.initials: M
authors.lastname: Schieker
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D002465:Cell Movement; D002470:Cell Survival; D058948:Cell Tracking / Q000379:methods*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism*; D009942:Organometallic Compounds / Q000378:metabolism*; D015125:Oxyquinoline / Q000031:analogs & derivatives* / Q000378:metabolism; D011923:Rats, Nude; D013194:Staining and Labeling; D015899:Tomography, Emission-Computed, Single-Photon; D014057:Tomography, X-Ray Computed
chemical_list: D009942:Organometallic Compounds; C032950:indium oxine; D015125:Oxyquinoline