title: Gene expression profiling of HUH7-ins: lack of a granulogenic function for chromogranin A.
abstract: Previously, the insulin producing liver cell line HUH7-ins has been shown to synthesize, store and secrete insulin in response to glucose via secretory granules. The current study characterized the gene expression profile of HUH7-ins with the aim to identify changes possibly involved in the formation of granules. Additionally, experiments were conducted to determine the influence of chromogranin A (CgA) on secretory granule biogenesis (SGB) in HUH7-ins. Expression of 165 genes were significantly changed in HUH7-ins,though interestingly the majority of secretory granule associated genes, such as the chromogranins were unchanged. CgA was over-expressed in glucose unresponsive HUH7-ins cells to test whether CgA played a role in SGB and would restore the regulated secretory phenotype. Over-expression affected neither the storage nor regulated secretion of insulin. These data suggest that SGB may by regulated at a post-transcriptional level with no evidence to indicate that CgA regulates SGB in the cell line HUH7-ins.
authors.affiliation: Diabetes Transplant Unit, Prince of Wales Hospital, Sydney, New South Wales, Australia.
authors.forename: Mark A
authors.initials: MA
authors.lastname: Lutherborrow
authors.forename: Mathiyalagan
authors.initials: M
authors.lastname: Appavoo
authors.forename: Ann M
authors.initials: AM
authors.lastname: Simpson
authors.forename: Bernard E
authors.initials: BE
authors.lastname: Tuch
mesh_terms: D045744:Cell Line, Tumor; D053379:Chromogranin A / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000502:physiology; D005947:Glucose / Q000378:metabolism; D006801:Humans; D007328:Insulin / Q000378:metabolism; D050417:Insulin-Secreting Cells / Q000737:chemistry / Q000378:metabolism*; D008297:Male; D046228:Microarray Analysis; D008875:Middle Aged; D022142:Secretory Vesicles / Q000235:genetics* / Q000378:metabolism; D054928:Validation Studies as Topic
chemical_list: D053379:Chromogranin A; D007328:Insulin; D005947:Glucose