title: Biochemical characterization of Pumilio1 and Pumilio2 in Xenopus oocytes.
abstract: Precise control of the timing of translational activation of dormant mRNAs stored in oocytes is required for normal progression of oocyte maturation. We previously showed that Pumilio1 (Pum1) is specifically involved in the translational control of cyclin B1 mRNA during Xenopus oocyte maturation, in cooperation with cytoplasmic polyadenylation element-binding protein (CPEB). It was reported that another Pumilio, Pumilio2 (Pum2), exists in Xenopus oocytes and that this protein regulates the translation of RINGO mRNA, together with Deleted in Azoospermia-like protein (DAZL). In this study, we characterized Pum1 and Pum2 biochemically by using newly produced antibodies that discriminate between them. Pum1 and Pum2 are bound to several key proteins involved in translational control of dormant mRNAs, including CPEB and DAZL, in immature oocytes. However, Pum1 and Pum2 themselves have no physical interaction. Injection of anti-Pum1 or anti-Pum2 antibody accelerated CPEB phosphorylation, cyclin B1 translation, and oocyte maturation. Pum1 phosphorylation coincides with the dissociation of CPEB from Pum1 and the translational activation of cyclin B1 mRNA, a target of Pum1, whereas Pum2 phosphorylation occurred at timing earlier than that for Pum1. Some, but not all, of cyclin B1 mRNAs release the deadenylase PARN during oocyte maturation, whereas Pum1 remains associated with the mRNA. On the basis of these findings, we discuss the functions of Pum1 and Pum2 in translational control of mRNAs during oocyte maturation.
authors.affiliation: Laboratory of Reproductive and Developmental Biology, Graduate School of Life Science, Hokkaido University, Sapporo 060-0810, Japan.
authors.forename: Ryoma
authors.initials: R
authors.lastname: Ota
authors.forename: Tomoya
authors.initials: T
authors.lastname: Kotani
authors.forename: Masakane
authors.initials: M
authors.lastname: Yamashita
mesh_terms: D000818:Animals; D000906:Antibodies / Q000737:chemistry; D001483:Base Sequence; D002352:Carrier Proteins / Q000096:biosynthesis / Q000235:genetics; D018797:Cell Cycle Proteins / Q000096:biosynthesis / Q000235:genetics; D056744:Cyclin B1 / Q000096:biosynthesis / Q000235:genetics; D008969:Molecular Sequence Data; D009865:Oocytes / Q000378:metabolism*; D010766:Phosphorylation / Q000502:physiology; D014176:Protein Biosynthesis / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D013997:Time Factors; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics; D029867:Xenopus Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D014982:Xenopus laevis; D039221:mRNA Cleavage and Polyadenylation Factors / Q000096:biosynthesis / Q000235:genetics
chemical_list: D000906:Antibodies; D002352:Carrier Proteins; D018797:Cell Cycle Proteins; C490388:Cpeb1 protein, Xenopus; D056744:Cyclin B1; C503588:Dazl protein, Xenopus; C430875:PUM1 protein, Xenopus; C508296:Pum2 protein, Xenopus; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D014157:Transcription Factors; D029867:Xenopus Proteins; C119055:ls27 protein, Xenopus; D039221:mRNA Cleavage and Polyadenylation Factors