title: The transcription elongation factor Bur1-Bur2 interacts with replication protein A and maintains genome stability during replication stress.
abstract: Multiple DNA-associated processes such as DNA repair, replication, and recombination are crucial for the maintenance of genome integrity. Here, we show a novel interaction between the transcription elongation factor Bur1-Bur2 and replication protein A (RPA), the eukaryotic single-stranded DNA-binding protein with functions in DNA repair, recombination, and replication. Bur1 interacted via its C-terminal domain with RPA, and bur1-ΔC mutants showed a deregulated DNA damage response accompanied by increased sensitivity to DNA damage and replication stress as well as increased levels of persisting Rad52 foci. Interestingly, the DNA damage sensitivity of an rfa1 mutant was suppressed by bur1 mutation, further underscoring a functional link between these two protein complexes. The transcription elongation factor Bur1-Bur2 interacts with RPA and maintains genome integrity during DNA replication stress.
authors.affiliation: Department of Biochemistry, Gene Center, Ludwig-Maximilians-Universität Münich, Feodor-Lynen-Strasse 25, 81377 Munich, Germany.
authors.forename: Emanuel
authors.initials: E
authors.lastname: Clausing
authors.forename: Andreas
authors.initials: A
authors.lastname: Mayer
authors.forename: Sittinan
authors.initials: S
authors.lastname: Chanarat
authors.forename: Barbara
authors.initials: B
authors.lastname: Müller
authors.forename: Susanne M
authors.initials: SM
authors.lastname: Germann
authors.forename: Patrick
authors.initials: P
authors.lastname: Cramer
authors.forename: Michael
authors.initials: M
authors.lastname: Lisby
authors.forename: Katja
authors.initials: K
authors.lastname: Strässer
mesh_terms: D000483:Alleles; D018844:Cyclin-Dependent Kinases / Q000737:chemistry*; D016213:Cyclins / Q000737:chemistry*; D004249:DNA Damage; D004261:DNA Replication; D016678:Genome; D055106:Genome-Wide Association Study; D008856:Microscopy, Fluorescence / Q000379:methods; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis; D025941:Protein Interaction Mapping; D017434:Protein Structure, Tertiary; D011995:Recombination, Genetic; D051716:Replication Protein A / Q000737:chemistry*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry*; D013696:Temperature
chemical_list: C414164:Bur2 protein, S cerevisiae; D016213:Cyclins; D051716:Replication Protein A; D029701:Saccharomyces cerevisiae Proteins; D018844:Cyclin-Dependent Kinases; C414163:SGV1 protein, S cerevisiae