title: Galectin-3 binding protein promotes cell motility in colon cancer by stimulating the shedding of protein tyrosine phosphatase kappa by proprotein convertase 5.
abstract: It has previously been reported that shedding of the PTPκ ectodomain drives enhanced motility of colon cancer cells. Herein, we provide mechanism underlying the regulation of PTPκ shedding by galectin-3 binding protein. PTPκ was inarguably scissored by the processed form of proprotein convertase 5 (subtilisin/kexin type 5), and galectin-3 binding protein which is over-produced in colon cancer cells and tissues contributed to increased cancer cell motility by acting as a negative regulator of galectin-3 at the cell surface. The high expression ratio of galectin-3 binding protein to galectin-3 was clinically correlated to lymphatic invasion. These results suggest that galectin-3 binding protein may be a potential therapeutic target for treatment of, at least, colon cancer patients with high expression of galectin-3 binding protein.
authors.affiliation: Daejeon-KRIBB-FHCRC Research Cooperation Center, KRIBB, Daejeon 305-806, South Korea.
authors.forename: Yong-Sam
authors.initials: YS
authors.lastname: Kim
authors.forename: Jee-Ae
authors.initials: JA
authors.lastname: Jung
authors.forename: Hyun-Jung
authors.initials: HJ
authors.lastname: Kim
authors.forename: Yeong Hee
authors.initials: YH
authors.lastname: Ahn
authors.forename: Jong Shin
authors.initials: JS
authors.lastname: Yoo
authors.forename: Sejeong
authors.initials: S
authors.lastname: Oh
authors.forename: Changhee
authors.initials: C
authors.lastname: Cho
authors.forename: Hyang-Sook
authors.initials: HS
authors.lastname: Yoo
authors.forename: Jeong-Heon
authors.initials: JH
authors.lastname: Ko
mesh_terms: D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D002352:Carrier Proteins / Q000378:metabolism*; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D003110:Colonic Neoplasms / Q000378:metabolism / Q000473:pathology*; D037502:Galectin 3 / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism*; D006801:Humans; D045702:Proprotein Convertase 5 / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D054623:Receptor-Like Protein Tyrosine Phosphatases, Class 2 / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D016326:Extracellular Matrix Proteins
chemical_list: D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D002352:Carrier Proteins; D037502:Galectin 3; D006023:Glycoproteins; D045702:Proprotein Convertase 5; D054623:Receptor-Like Protein Tyrosine Phosphatases, Class 2; C081856:LGALS3BP protein, human; D016326:Extracellular Matrix Proteins