title: Inhibition of protein adsorption and cell adhesion on PNIPAAm-grafted polyurethane surface: effect of graft molecular weight.
abstract: In this work, the effect of molecular weight (MW) of surface grafted poly(N-isopropylacrylamide) (PNIPAAm) on protein adsorption and cell adhesion was investigated systematically. PNIPAAm-grafted polyurethane (PU) surfaces of varying graft MW were prepared via conventional radical polymerization. The MW was controlled by adjusting the monomer concentration. Fibrinogen (Fg) and human serum albumin (HSA) were selected as model proteins and their adsorption from phosphate-buffered saline (PBS, pH 7.4) and blood plasma at 37°C was measured using a radiolabeling method and immunoblot analysis respectively. It was found that in both media, as the MW increased, the adsorption of these two proteins decreased gradually reaching a plateau value at MW above 7.9×10(4). Compared to the unmodified PU, the surface grafted with PNIPAAm of MW 14.6×10(4) reduced the adsorption of Fg and HSA in PBS by 91% and 86%, respectively. Moreover, the surfaces with higher MW PNIPAAm showed minimal adhesion of L929 cells presumably due to the absence of cell-adhesive proteins on the surfaces.
authors.affiliation: College of Chemistry, Chemical Engineering and Materials Science, Soochow University, 199 Ren'ai Road, Suzhou, PR China.
authors.forename: Tieliang
authors.initials: T
authors.lastname: Zhao
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
authors.forename: Jun
authors.initials: J
authors.lastname: Zheng
authors.forename: Qian
authors.initials: Q
authors.lastname: Yu
authors.forename: Zhaoqiang
authors.initials: Z
authors.lastname: Wu
authors.forename: Lin
authors.initials: L
authors.lastname: Yuan
mesh_terms: D000178:Acrylamides / Q000737:chemistry* / Q000494:pharmacology; D000180:Acrylic Resins; D000327:Adsorption; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D051379:Mice; D015394:Molecular Structure; D008970:Molecular Weight; D011108:Polymers / Q000737:chemistry* / Q000494:pharmacology; D011140:Polyurethanes / Q000737:chemistry* / Q000494:pharmacology; D013499:Surface Properties; D017155:Wettability
chemical_list: D000178:Acrylamides; D000180:Acrylic Resins; D011108:Polymers; D011140:Polyurethanes; C052970:poly-N-isopropylacrylamide