title: Coordination properties of zinc finger peptides revisited: ligand competition studies reveal higher affinities for zinc and cobalt.
abstract: Zinc fingers are ubiquitous small protein domains which have a Zn(Cys)(4-x)(His)(x) site. They possess great diversity in their structure and amino acid composition. Using a family of six peptides, it was possible to assess the influence of hydrophobic amino acids on the metal-peptide affinities and on the rates of metal association and dissociation. A model of a treble-clef zinc finger, a model of the zinc finger site of a redox-switch protein, and four variants of the classical ββα zinc finger were used. They differ in their coordination set, their sequence length, and their hydrophobic amino acid content. The speciation, metal binding constants, and structure of these peptides have been investigated as a function of pH. The zinc binding constants of peptides, which adopt a well-defined structure, were found to be around 10(15) at pH 7.0. The rates of zinc exchange between EDTA and the peptides were also assessed. We evidenced that the packing of hydrophobic amino acids into a well-defined hydrophobic core can have a drastic influence on both the binding constant and the kinetics of metal exchange. Notably, well-packed hydrophobic amino acids can increase the stability constant by 4 orders of magnitude. The half-life of zinc exchange was also seen to vary significantly depending on the sequence of the zinc finger. The possible causes for this behavior are discussed. This work will help in understanding the dynamics of zinc exchange in zinc-containing proteins.
authors.affiliation: Laboratoire de Chimie et Biologie des Métaux, CEA/iRTSV/LCBM, UMR 5249 CNRS/Université Joseph Fourier/CEA-Grenoble, 17 rue des Martyrs, 38054 Grenoble, France. olivier.seneque@cea.fr
authors.forename: Olivier
authors.initials: O
authors.lastname: Sénèque
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Latour
mesh_terms: D000595:Amino Acid Sequence; D001667:Binding, Competitive; D003035:Cobalt / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D008024:Ligands; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry*; D015032:Zinc / Q000737:chemistry*; D016335:Zinc Fingers
chemical_list: D008024:Ligands; D010455:Peptides; D003035:Cobalt; D015032:Zinc