title: Human DBS sampling with LC-MS/MS for enantioselective determination of metoprolol and its metabolite O-desmethyl metoprolol.
abstract: BACKGROUND
  The dried blood spots (DBS) sampling technique has been gaining wide interest in preclinical and clinical studies due to its inherent advantages. However, the impact of DBS sampling on chiral compounds in terms of stability and detection sensitivity has not been studied yet.
  
  
  RESULTS
  A high-throughput, sensitive and enantioselective LC-MS/MS-based bioanalytical method was developed and validated for the simultaneous determination of individual enantiomers of metoprolol and its metabolite O-desmethylmetoprolol (O-DMM)in human whole blood using the DBS sampling technique.
  
  
  CONCLUSIONS
  The developed DBS LC-MS/MS assay has a run time of 3 min, shorter than all previous methods while achieving complete baseline separation of enantiomeric metoprolol and O-DMM. This study demonstrates the applicability of DBS for chiral molecules analysis.
authors.affiliation: Department of Bioanalytical Chemistry, Covance Laboratories Inc. 3301 Kinsman Blvd, Madison, WI 53704, USA. xiaorong.liang@covance.com
authors.forename: Xiaorong
authors.initials: X
authors.lastname: Liang
authors.forename: Hongliang
authors.initials: H
authors.lastname: Jiang
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
mesh_terms: D001774:Blood Chemical Analysis / Q000379:methods*; D001800:Blood Specimen Collection / Q000379:methods* / Q000592:standards; D001810:Blood Volume; D002853:Chromatography, Liquid / Q000379:methods*; D003890:Desiccation; D004355:Drug Stability; D006801:Humans; D016014:Linear Models; D008790:Metoprolol / Q000031:analogs & derivatives* / Q000097:blood* / Q000737:chemistry* / Q000378:metabolism; D011786:Quality Control; D013237:Stereoisomerism; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013696:Temperature
chemical_list: C556565:O-desmethyl metoprolol; D008790:Metoprolol