title: Cyclic phosphatidic acid influences the expression and regulation of cyclic nucleotide phosphodiesterase 3B and lipolysis in 3T3-L1 cells.
abstract: Cyclic phosphatidic acid (cPA) is found in cells from slime mold to humans and has a largely unknown function. We previously reported that cPA significantly inhibited the lipid accumulation in 3T3-L1 adipocytes through inhibition of PPARγ activation. We find here that cPA reduced intracellular triglyceride levels and inhibited the phosphodiesterase 3B (PDE3B) expression in 3T3-L1 adipocytes. PPARγ activation in adipogenesis that can be blocked by treatment with cPA then participates in adipocyte function through inhibition of PDE3B expression. We also found the intracellular cAMP levels in 3T3-L1 adipocytes increased after exposure to cPA. These findings contribute to the participation of cPA on the lipolytic activity in 3T3-L1 adipocytes. Our studies imply that cPA might be a therapeutic compound in the treatment of obesity and obesity-related diseases.
authors.affiliation: Department of Integrative Physiology and Bio-System Control, Shinshu University School of Medicine, 3-1-1 Asahi, Matsumoto, Nagano 390-8621, Japan. ttamotsu@shinshu-u.ac.jp
authors.forename: Tamotsu
authors.initials: T
authors.lastname: Tsukahara
authors.forename: Shuwa
authors.initials: S
authors.lastname: Hanazawa
authors.forename: Kimiko
authors.initials: K
authors.lastname: Murakami-Murofushi
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000187:drug effects* / Q000201:enzymology; D050156:Adipogenesis / Q000187:drug effects*; D000818:Animals; D000242:Cyclic AMP / Q000096:biosynthesis; D054684:Cyclic Nucleotide Phosphodiesterases, Type 3 / Q000235:genetics / Q000378:metabolism*; D006573:Heterocyclic Compounds, 1-Ring / Q000378:metabolism / Q000494:pharmacology*; D008066:Lipolysis / Q000187:drug effects*; D051379:Mice; D047495:PPAR gamma / Q000037:antagonists & inhibitors; D010712:Phosphatidic Acids / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D006573:Heterocyclic Compounds, 1-Ring; D047495:PPAR gamma; D010712:Phosphatidic Acids; C555818:cyclic phosphatidic acid 16:0; D000242:Cyclic AMP; D054684:Cyclic Nucleotide Phosphodiesterases, Type 3