title: Promising multiple-epitope recombinant vaccine against foot-and-mouth disease virus type O in swine.
abstract: In order to develop a completely safe immunogen to replace the traditional inactivated vaccine, a tandem-repeat multiple-epitope recombinant vaccine against foot-and-mouth disease (FMD) virus (FMDV) type O was developed. It contained three copies each of residues 141 to 160 and 200 to 213 of VP1 of the O/China/99 strain of FMDV coupled with a swine immunoglobulin G heavy-chain constant region (scIgG). The data showed that the multiple-epitope recombinant vaccine elicited high titers of anti-FMDV specific antibodies in swine at 30 days postvaccination (dpv) and conferred complete protection against a challenge with 10³ 50% swine infective doses of the O/China/99 strain. The anti-FMDV specific antibody titers were not significantly different between the multiple-epitope recombinant vaccine and the traditional vaccine (t test, P > 0.05). The number of 50% pig protective doses was 6.47, which is higher than the number recommended by the World Organization for Animal Health. The multiple-epitope recombinant vaccine resulted in a duration of immunity of at least 6 months. We speculate that the multiple-epitope recombinant vaccine is a promising vaccine that may replace the traditional inactivated vaccine for the prevention and control of FMD in swine in the future.
authors.affiliation: Key Laboratory of Animal Virology, Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 730046 Lanzhou, China.
authors.forename: Jun-Jun
authors.initials: JJ
authors.lastname: Shao
authors.forename: Chung Kai
authors.initials: CK
authors.lastname: Wong
authors.forename: Tong
authors.initials: T
authors.lastname: Lin
authors.forename: Shuk Kwan
authors.initials: SK
authors.lastname: Lee
authors.forename: Guo-Zheng
authors.initials: GZ
authors.lastname: Cong
authors.forename: Fion Wai Yee
authors.initials: FW
authors.lastname: Sin
authors.forename: Jun-Zheng
authors.initials: JZ
authors.lastname: Du
authors.forename: Shan-Dian
authors.initials: SD
authors.lastname: Gao
authors.forename: Xiang-Tao
authors.initials: XT
authors.lastname: Liu
authors.forename: Xue-Peng
authors.initials: XP
authors.lastname: Cai
authors.forename: Yong
authors.initials: Y
authors.lastname: Xie
authors.forename: Hui-Yun
authors.initials: HY
authors.lastname: Chang
authors.forename: Ji-Xing
authors.initials: JX
authors.lastname: Liu
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D036022:Capsid Proteins / Q000235:genetics / Q000276:immunology; D002681:China; D000939:Epitopes / Q000235:genetics / Q000276:immunology*; D005536:Foot-and-Mouth Disease / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D029544:Foot-and-Mouth Disease Virus / Q000145:classification / Q000276:immunology*; D007127:Immunoglobulin Constant Regions / Q000235:genetics / Q000276:immunology; D013552:Swine; D013553:Swine Diseases / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D014614:Vaccines, Synthetic / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D036022:Capsid Proteins; D000939:Epitopes; D007127:Immunoglobulin Constant Regions; C442501:VP1 protein, Foot-and-mouth disease virus; D014614:Vaccines, Synthetic; D014765:Viral Vaccines