title: Depsipeptides from a Guamanian Marine Cyanobacterium, Lyngbya bouillonii, with Selective Inhibition of Serine Proteases.
abstract: Bouillomides A (1) and B (2) are two depsipeptide analogues of dolastatin 13. Isolated from a Guamanian sample of Lyngbya bouillonii, the planar structures were elucidated on the basis of HR-ESI-MS and NMR data, while the absolute configurations were determined by employing functional group conversions, modified Marfey's analysis, and detailed analyses of ROESY correlations. Compounds 1 and 2 selectively inhibited serine proteases elastase (IC(50) = 1.9 μM for both) and chymotrypsin (IC(50) = 0.17 and 9.3 μM, respectively) while showing no inhibition of trypsin (IC(50) > 100 μM).
authors.affiliation: Department of Chemistry, University of Hawai'i at Manoa, Honolulu, Hawai'i, 96822, The Cancer Research Center of Hawai'i, 651 Ilalo Street, Honolulu, Hawai'i, 96813, and University of Guam, UOG Marine Station, Mangilao, Guam, 96923.
authors.forename: Brent K
authors.initials: BK
authors.lastname: Rubio
authors.forename: Stephen M
authors.initials: SM
authors.lastname: Parrish
authors.forename: Wesley
authors.initials: W
authors.lastname: Yoshida
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Schupp
authors.forename: Tom
authors.initials: T
authors.lastname: Schils
authors.forename: Philip G
authors.initials: PG
authors.lastname: Williams