title: Pertussis serology: assessment of IgG anti-PT ELISA for replacement of the CHO cell assay.
abstract: Two types of serological assays are commonly used for the assessment of pertussis vaccine-induced antibodies; the Chinese hamster ovary cell (CHO cell) assay and the immunoglobulin G (IgG) anti pertussis toxin (PT) enzyme-linked immunosorbent assay (IgG anti-PT ELISA). Recently, both the techniques have been modified to improve performance with sera with interfering activity (CHO cell assay) or with heat-treated sera (IgG anti-PT ELISA). These two improved techniques were compared by the analysis of 100 individual serum samples from a previous clinical trial and 213 sera from a longitudinal serum collection from 20 Danish adults recently vaccinated with the Danish acellular pertussis vaccine. The comparison showed a significant linear correlation between the results of the two assays with a p-value of <0.0001 for the 100 individual samples. We, therefore, conclude that the improved IgG anti-PT ELISA can be used as a replacement for the often troublesome and time-consuming CHO cell assay for the measurement of vaccine-induced human antibodies to PT.
authors.affiliation: Department of Microbiological Surveillance and Research, Statens Serum Institut, Copenhagen, Denmark. tid@ssi.dk
authors.forename: Tine
authors.initials: T
authors.lastname: Dalby
authors.forename: Charlotte
authors.initials: C
authors.lastname: Sørensen
authors.forename: Jesper Westphal
authors.initials: JW
authors.lastname: Petersen
authors.forename: Karen Angeliki
authors.initials: KA
authors.lastname: Krogfelt
mesh_terms: D000818:Animals; D001886:Bordetella pertussis / Q000276:immunology*; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D037342:Pertussis Toxin / Q000276:immunology*; D010567:Pertussis Vaccine / Q000276:immunology*; D018709:Statistics, Nonparametric; D014917:Whooping Cough / Q000097:blood / Q000276:immunology* / Q000517:prevention & control
chemical_list: D007074:Immunoglobulin G; D010567:Pertussis Vaccine; D037342:Pertussis Toxin