title: The immediately releasable vesicle pool: highly coupled secretion in chromaffin and other neuroendocrine cells.
abstract: In neuroendocrine cells, such as adrenal chromaffin cells, the exocytosis of hormone-filled vesicles is triggered by a localized Ca(2+) increase that develops after the activation of voltage-dependent Ca(2+) channels. To reach the fusion competent state, vesicles have to go through a series of maturation steps that involve the detachment from cytoskeletal proteins, docking and priming. However, the fusion readiness of vesicles will also depend on their proximity to the calcium source. The immediately releasable pool is a small group of ready-to-fuse vesicles, whose fusion is tightly coupled to Ca(2+) entry through channels. Recent work indicates that such coupling is not produced by a random distribution between vesicles and channels, but would be the result of a specific interaction of immediately releasable vesicles with particular Ca(2+) channel subtypes. The immediately releasable pool is able to sustain, with high efficiency, the secretion triggered by the small and localized Ca(2+) gradients produced by brief depolarizations at low frequencies, like action potentials at basal conditions in adrenal chromaffin cells.
authors.affiliation: Laboratorio de Fisiología y Biología Molecular, Instituto de Fisiología, Biología Molecular y Neurociencias (CONICET), Facultad de Ciencias Exactas y Naturales, Departamento de Fisiología, Biología Molecular y Celular, Universidad de Buenos Aires, Buenos Aires, Argentina.
authors.forename: Yanina D
authors.initials: YD
authors.lastname: Alvarez
authors.forename: Fernando D
authors.initials: FD
authors.lastname: Marengo
mesh_terms: D000818:Animals; D015220:Calcium Channels / Q000378:metabolism; D019439:Chromaffin Cells / Q000378:metabolism*; D005089:Exocytosis / Q000502:physiology*; D006801:Humans; D055099:Neuroendocrine Cells / Q000166:cytology / Q000378:metabolism*; D013572:Synaptic Vesicles / Q000378:metabolism*; D013997:Time Factors
chemical_list: D015220:Calcium Channels