title: Single-molecule imaging of protein adsorption mechanisms to surfaces.
abstract: Protein-surface interactions cause the desirable effect of controlled protein adsorption onto biodevices as well as the undesirable effect of protein fouling. The key to controlling protein-surface adsorptions is to identify and quantify the main adsorption mechanisms: adsorptions that occur (1) while depositing a protein solution onto dry surfaces and (2) after the deposition onto wet surfaces. Bulk measurements cannot reveal the dynamic protein adsorption pathways and thus cannot differentiate between the two adsorption mechanisms. We imaged the interactions of single streptavidin molecules with hydrophobic fused-silica surfaces in real-time. We observed both adsorbed proteins on surfaces and diffusing proteins near surfaces and analyzed their adsorption kinetics. Our analysis shows that the protein solution deposition process is the primary mechanism of streptavidin adsorption onto surfaces at the subnanomolar to nanomolar protein concentrations. Furthermore, we found that hydrophilic fused-silica surfaces can prevent the adsorption of streptavidin molecules.
authors.affiliation: Department of Physics, Washington University, St Louis, Missouri 63130, USA.
authors.forename: Shannon Kian
authors.initials: SK
authors.lastname: Zareh
authors.forename: Yan Mei
authors.initials: YM
authors.lastname: Wang
mesh_terms: D000327:Adsorption; D004058:Diffusion; D005456:Fluorescent Dyes / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D007700:Kinetics; D008856:Microscopy, Fluorescence / Q000379:methods*; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism; D012822:Silicon Dioxide / Q000737:chemistry; D019809:Streptavidin / Q000737:chemistry* / Q000378:metabolism; D013499:Surface Properties
chemical_list: D005456:Fluorescent Dyes; D011506:Proteins; D012822:Silicon Dioxide; D019809:Streptavidin