title: Genetic mutations of p53 and k-ras in gastric carcinoma patients from Hunan, China.
abstract: This case-control study investigated the mutations in p53 and k-ras genes of 123 gastric carcinoma patients and 129 normal individuals from Hunan, China. By isolating genomic DNA from peripheral blood and employing polymerase chain reaction-single strand conformation polymorphism and DNA sequencing, the mutations of p53 exons-5, 6, 7, and 8 and k-ras were detected. The overall mutation frequency of p53 was 29.3%, and mutation was found in all four exons studied. The point mutations were predominant and among them, G:C→A:T was the highest (41.7%), followed by A:T→G:C (25%), G:C→C:G (11.1%), G:C→T:A (8.3%), and A:T→T:A (2.8%). The frameshift mutation was 11.1%. Mutations were detected in codons-131, 132, 133, 135, 149, 151, 162, 167, 173, 174, and 175 of exon 5, codons-193, 197, 213, and 215 of exon 6, codons-245, 246, 248, 249, and 270 of exon 7, and codons-271, 272, 273, and 282 of exon 8 of p53. The overall frequency of mutation in k-ras was 9.8%, mostly in codon-12 (91.7%) and in codon-13 (8.3%). There was no significant relationship between p53 and k-ras gene mutation in gastric carcinoma patients. Also, the relationships between p53 mutation and age, sex, smoking or drinking, and tumor metastasis were not significant. However, the patients with high/high-middle differentiated gastric carcinoma had a higher association with of p53 mutations. This study identified some novel p53 mutations in gastric cancer and showed mutation pattern and frequency of p53 and k-ras in the population of the central southern region of China.
authors.affiliation: Department of Biochemistry, School of Biological Science and Technology, Central South University, Changsha, Hunan 410013, China. chenhanchun@mail.csu.edu.cn
authors.forename: Han-Chun
authors.initials: HC
authors.lastname: Chen
authors.forename: Hui-Juan
authors.initials: HJ
authors.lastname: Chen
authors.forename: Md Asaduzzaman
authors.initials: MA
authors.lastname: Khan
authors.forename: Zhou-Zhou
authors.initials: ZZ
authors.lastname: Rao
authors.forename: Xin-Xing
authors.initials: XX
authors.lastname: Wan
authors.forename: Bo
authors.initials: B
authors.lastname: Tan
authors.forename: Dian-Zheng
authors.initials: DZ
authors.lastname: Zhang
mesh_terms: D000328:Adult; D000368:Aged; D000483:Alleles; D016022:Case-Control Studies; D002681:China; D003062:Codon / Q000235:genetics; D005091:Exons / Q000235:genetics; D005260:Female; D016368:Frameshift Mutation / Q000235:genetics*; D016158:Genes, p53 / Q000235:genetics*; D011905:Genes, ras / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D017354:Point Mutation / Q000235:genetics*; D013274:Stomach Neoplasms / Q000208:ethnology* / Q000235:genetics*
chemical_list: D003062:Codon