title: [Ovine Follistatin gene expression and functional analysis of follistatin domains].
abstract: In order to study ovine follistatin function, we amplified the total of 1038 base pair of ovine complete follistatin cDNA and cloned into pGEM-T vector by RT-PCR from ovine ovary RNA. After removal of the signal peptide it was subcloned into the pET41a to construct the prokaryotic expression vector, named pFSsig-. SDS-PAGE and Western blotting identified the 66 kDa product of the expression of follistatin cDNA. Based on the complete CDS sequence, we cloned follistatin N-terminal domain and domain 1 with PCR and inserted into pLEX-MCS lentiviral vector, named pFS-N+D1. After package and passage of lentivirus in 293T cells, and then infected sheep primary muscle cells (SPMC). The expression of FS N+D1 in SPMC was assayed by Western blotting. The cell growth curve of the infected SPMC and noninfected control cells displayed that FS N+D1 stablly transfected SPMC proliferated significantly faster than the control cells (P < 0.01). Our data inferred that ovine FS N+D1 domain had the function to stimulate sheep muscle cell growth.
authors.affiliation: Key Laboratory of Animal Biotechnology of Xinjiang, Urumqi 830000, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Zhang
authors.forename: Xuemei
authors.initials: X
authors.lastname: Zhang
authors.forename: Mingjun
authors.initials: M
authors.lastname: Liu
authors.forename: Lixin
authors.initials: L
authors.lastname: Tan
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005260:Female; D038681:Follistatin / Q000096:biosynthesis* / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics*; D016086:Lentivirus / Q000235:genetics / Q000378:metabolism; D018482:Muscle, Skeletal / Q000166:cytology; D010053:Ovary / Q000378:metabolism; D011387:Prokaryotic Cells / Q000378:metabolism; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D012756:Sheep
chemical_list: D038681:Follistatin; D011994:Recombinant Proteins