title: [Effects of AMPK on the transcriptional activity of FOXO1 and ubiquitin ligase MuRF1 expression in rat cardiomyocytes].
abstract: OBJECTIVE
  To investigate the effects of AICAR on the activity of transcription factor FOXO1 and expression of ubiquitin ligase MuRF1 in rat cardiomyocytes, and explore the possible role of AMP-activated protein kinase (AMPK) in proteolysis pathways.
  
  
  METHODS
  In vitro cultured neonatal rat cardiac myocytes were treated with AICAR, and Western blotting was used to detect the phosphorylation of FOXO1 and expression of MuRF1 in the cells.
  
  
  RESULTS
  AICAR activated AMPK in rat cardiac myocytes. Activated AMPK significantly inhibited the phosphorylation of FOXO1 and increased MuRF1 protein expression.
  
  
  CONCLUSIONS
  AMPK may regulate proteolysis by activating FOXO1 transcription factor and up-regulating MuRF1 expression.
authors.affiliation: Department of Cardiology, People's Hospital of Guizhou Province, Guiyang 550002, China. chenbl104@126.com
authors.forename: Bao-lin
authors.initials: BL
authors.lastname: Chen
authors.forename: Zhao-jun
authors.initials: ZJ
authors.lastname: Xiong
authors.forename: Cheng-xi
authors.initials: CX
authors.lastname: Zhang
authors.forename: Yue-dong
authors.initials: YD
authors.lastname: Ma
authors.forename: Rong-sen
authors.initials: RS
authors.lastname: Meng
authors.forename: Guang-qin
authors.initials: GQ
authors.lastname: Chen
authors.forename: Chen
authors.initials: C
authors.lastname: Liu
authors.forename: Yu-gang
authors.initials: YG
authors.lastname: Dong
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000378:metabolism*; D000620:Aminoimidazole Carboxamide / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D002478:Cells, Cultured; D051858:Forkhead Transcription Factors / Q000378:metabolism*; D009124:Muscle Proteins / Q000378:metabolism*; D032383:Myocytes, Cardiac / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012265:Ribonucleotides / Q000494:pharmacology; D000070584:Tripartite Motif Proteins; D044767:Ubiquitin-Protein Ligases / Q000378:metabolism*; D000071161:Forkhead Box Protein O1
chemical_list: D055372:AMP-Activated Protein Kinases; D000620:Aminoimidazole Carboxamide; D051858:Forkhead Transcription Factors; D009124:Muscle Proteins; D009419:Nerve Tissue Proteins; D012265:Ribonucleotides; D000070584:Tripartite Motif Proteins; D044767:Ubiquitin-Protein Ligases; C074639:Foxo1 protein, rat; D000071161:Forkhead Box Protein O1; C499086:Trim63 protein, rat; C031143:AICA ribonucleotide