title: Cell wall glycoproteins participate in the adhesion of Sporothrix schenckii to epithelial cells.
abstract: Sporothrix schenckii is the etiologic agent of sporotrichosis. This fungal infection is an emerging disease potentially fatal in immunocompromised patients. The adhesion to host cells is a crucial early event related with the dissemination of pathogens. In order to clarify the mechanisms of adhesion of S. schenckii yeast cell to epithelial cells, we studied the biochemical basis of this process. The electrophoretic analysis of cell wall protein from S. schenckii coupled at ConA and stained with HRP, revealed nine different proteins with MW ≥ 180, 115, 90, 80, 58, 40, 36, 22 and 18 kDa. Using ligand-like assay with biotinylated S. schenckii surface proteins, five proteins with MW ≥ 190, 180, 115, 90 and 80 kDa which have affinity to epithelial cells were identified. The adhesion of yeast to epithelial monolayer was significantly inhibited when S. schenckii was pretreated with concanavalinA (ConA) and wheat germ agglutinin (WGA) lectins, alkali, periodate, trypsin, endoglycosidase H (EndoH), salt solutions and detergents. The ability of adhesion of S. schenckii yeast was recovered by blocking the lectin with sugar complementary. These data suggest that surface glycoprotein with mannose and glucose residue could be participate in the process of fungal adhesion to epithelial cells.
authors.affiliation: División de Ciencias Naturales y Exactas Campus Guanajuato, Departamento de Biología, Universidad de Guanajuato, Noria Alta S/N, Col. Noria Alta, 36000, Guanajuato, Guanajuato, México.
authors.forename: Gerardo
authors.initials: G
authors.lastname: Sandoval-Bernal
authors.forename: Gloria
authors.initials: G
authors.lastname: Barbosa-Sabanero
authors.forename: Mineko
authors.initials: M
authors.lastname: Shibayama
authors.forename: Armando
authors.initials: A
authors.lastname: Perez-Torres
authors.forename: Víctor
authors.initials: V
authors.lastname: Tsutsumi
authors.forename: Myrna
authors.initials: M
authors.lastname: Sabanero
mesh_terms: D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D002460:Cell Line; D002473:Cell Wall / Q000737:chemistry*; D004586:Electrophoresis; D004847:Epithelial Cells / Q000382:microbiology*; D006023:Glycoproteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D008970:Molecular Weight; D013175:Sporothrix / Q000737:chemistry / Q000378:metabolism* / Q000502:physiology*
chemical_list: D015815:Cell Adhesion Molecules; D006023:Glycoproteins