title: Amino acid homeostasis modulates salicylic acid-associated redox status and defense responses in Arabidopsis.
abstract: The tight association between nitrogen status and pathogenesis has been broadly documented in plant-pathogen interactions. However, the interface between primary metabolism and disease responses remains largely unclear. Here, we show that knockout of a single amino acid transporter, LYSINE HISTIDINE TRANSPORTER1 (LHT1), is sufficient for Arabidopsis thaliana plants to confer a broad spectrum of disease resistance in a salicylic acid-dependent manner. We found that redox fine-tuning in photosynthetic cells was causally linked to the lht1 mutant-associated phenotypes. Furthermore, the enhanced resistance in lht1 could be attributed to a specific deficiency of its main physiological substrate, Gln, and not to a general nitrogen deficiency. Thus, by enabling nitrogen metabolism to moderate the cellular redox status, a plant primary metabolite, Gln, plays a crucial role in plant disease resistance.
authors.affiliation: Department of Biology, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E2, Canada.
authors.forename: Guosheng
authors.initials: G
authors.lastname: Liu
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Ji
authors.forename: Nazmul H
authors.initials: NH
authors.lastname: Bhuiyan
authors.forename: Guillaume
authors.initials: G
authors.lastname: Pilot
authors.forename: Gopalan
authors.initials: G
authors.lastname: Selvaraj
authors.forename: Jitao
authors.initials: J
authors.lastname: Zou
authors.forename: Yangdou
authors.initials: Y
authors.lastname: Wei
mesh_terms: D026923:Amino Acid Transport Systems, Basic / Q000235:genetics / Q000378:metabolism*; D000596:Amino Acids / Q000378:metabolism*; D017360:Arabidopsis / Q000033:anatomy & histology / Q000235:genetics / Q000276:immunology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D055785:Gene Knockdown Techniques; D005973:Glutamine / Q000378:metabolism; D006706:Homeostasis; D007113:Immunity, Innate / Q000235:genetics / Q000276:immunology*; D046228:Microarray Analysis; D009584:Nitrogen / Q000378:metabolism; D010084:Oxidation-Reduction; D010935:Plant Diseases / Q000235:genetics / Q000276:immunology*; D030821:Plants, Genetically Modified; D026361:Reactive Nitrogen Species / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D020156:Salicylic Acid / Q000378:metabolism*
chemical_list: D026923:Amino Acid Transport Systems, Basic; D000596:Amino Acids; D029681:Arabidopsis Proteins; C110638:LHT1 protein, Arabidopsis; D026361:Reactive Nitrogen Species; D017382:Reactive Oxygen Species; D011993:Recombinant Fusion Proteins; D005973:Glutamine; D009584:Nitrogen; D020156:Salicylic Acid