title: Exogenous L-arginine and HDL can alter LDL and ox-LDL-mediated platelet activation: using platelet P-selectin receptor numbers.
abstract: The aim of this study is to investigate the effects of exogenous L-arginine and HDL on LDL and oxidized LDL (ox-LDL)-mediated platelet activation. Adenosine diphosphate (ADP)-activated platelets have been incubated with lipoproteins with or without L-arginine. P-selectin receptor numbers per platelet have been measured by flow cytometry. After incubation with only L-arginine (without lipoproteins), platelet nitric oxide (NO) levels and P-selectin receptor numbers significantly increased compared to the controls (P < .05). After incubation with LDL or ox-LDL, receptor numbers of P-selectin significantly increased (P < .001). However, P-selectin receptor numbers in platelets treated with L-arginine + LDL or L-arginine + ox-LDL decreased compared to the levels in platelets treated with only LDL or ox-LDL (P < .01, P < .001, respectively). Addition of HDL to L-arginine + ox-LDL caused significant reduction in P-selectin receptor numbers as in the control values (P < .001).We have concluded that L-arginine causes enhanced platelet NO levels and blocks the effects of LDL or ox-LDL on platelet P-selectin receptor numbers and HDL also strengthens this effect of L-arginine.
authors.affiliation: Department of Biochemistry, Faculty of Pharmacy, Marmara University, Haydarpaşa, Istanbul, Turkey. azizesener@gmail.com
authors.forename: Azize
authors.initials: A
authors.lastname: Sener
authors.forename: Elif
authors.initials: E
authors.lastname: Enc
authors.forename: Derya
authors.initials: D
authors.lastname: Ozsavci
authors.forename: Birgul
authors.initials: B
authors.lastname: Vanizor-Kural
authors.forename: Gulderen
authors.initials: G
authors.lastname: Yanikkaya-Demirel
authors.forename: Rabia
authors.initials: R
authors.lastname: Oba
authors.forename: Fikriye
authors.initials: F
authors.lastname: Uras
authors.forename: Muzaffer
authors.initials: M
authors.lastname: Demir
mesh_terms: D000328:Adult; D001120:Arginine / Q000494:pharmacology*; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D002478:Cells, Cultured; D008078:Cholesterol, LDL / Q000097:blood / Q000494:pharmacology; D004347:Drug Interactions; D006801:Humans; D008074:Lipoproteins / Q000097:blood / Q000494:pharmacology*; D008075:Lipoproteins, HDL / Q000097:blood / Q000494:pharmacology*; D008077:Lipoproteins, LDL / Q000097:blood / Q000494:pharmacology*; D009569:Nitric Oxide / Q000097:blood; D019007:P-Selectin / Q000097:blood*; D015539:Platelet Activation / Q000187:drug effects*; D055815:Young Adult
chemical_list: D008078:Cholesterol, LDL; D008074:Lipoproteins; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D019007:P-Selectin; C099252:oxidized low density lipoprotein; D009569:Nitric Oxide; D001120:Arginine