title: Cloning and functional analysis of a new laccase gene from Trametes sp. 48424 which had the high yield of laccase and strong ability for decolorizing different dyes.
abstract: The laccase gene lac48424-1 and its corresponding full-length cDNA were cloned and characterized from a novel white-rot fungi Trametes sp. 48424 which had the high yield of laccase and strong ability for decolorizing different dyes. The 1563 bp full-length cDNA of lac48424-1 encoded a mature laccase protein containing 499 amino acids preceded by a signal peptide of 21 amino acids. The deduced protein sequence of LAC48424-1 showed high similarity with other known fungal laccases and contained four copper-binding conserved domains of typical laccase protein. The functionality of lac48424-1 gene encoding active laccase was verified by expressing the gene in the yeast Pichia pastoris successfully. It was found that the recombinant laccase produced by the yeast transformant could decolorize different dyes. The 5'-flanking sequence upstream of start codon was obtained by Self-Formed Adaptor PCR. Many putative cis-acting responsive elements involved in the transcriptional regulation were identified in the promoter region of lac48424-1.
authors.affiliation: College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: Fangfang
authors.initials: F
authors.lastname: Fan
authors.forename: Rui
authors.initials: R
authors.lastname: Zhuo
authors.forename: Su
authors.initials: S
authors.lastname: Sun
authors.forename: Xia
authors.initials: X
authors.lastname: Wan
authors.forename: Mulan
authors.initials: M
authors.lastname: Jiang
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Zhang
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
mesh_terms: D003116:Color; D004396:Coloring Agents / Q000737:chemistry*; D004789:Enzyme Activation; D004795:Enzyme Stability; D042845:Laccase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D015202:Protein Engineering / Q000379:methods; D055454:Trametes / Q000235:genetics / Q000378:metabolism*
chemical_list: D004396:Coloring Agents; D042845:Laccase