title: Gene expression in IFNß signalling pathway differs between monocytes, CD4 and CD8 T cells from MS patients.
abstract: IFNß exerts its activity through the interaction with IFNAR, through activation of the JAK/STAT pathway. We analyzed the changes in IFNAR1, IFNAR2, STAT1, STAT2, Tyk2, JAK1, IRF9 and MxA gene expressions after prolonged IFNß treatment, in isolated mononuclear-cell subpopulations from MS patients, by real time PCR. The effect of IFNß on gene expression differed depending on the subpopulation assessed. The data suggest that CD8+ T cells are the most influenced by prolonged IFNß therapy as IFNAR2, Tyk2, IRF9 and Jak1 expressions were decreased, whereas MxA expression was increased in these cells.
authors.affiliation: Research Laboratory, Hospital Civil, 29009 Málaga, Spain. begoliver@gmail.com
authors.forename: B
authors.initials: B
authors.lastname: Oliver-Martos
authors.forename: T
authors.initials: T
authors.lastname: Órpez
authors.forename: M J
authors.initials: MJ
authors.lastname: Pinto-Medel
authors.forename: C
authors.initials: C
authors.lastname: Mayorga
authors.forename: J A
authors.initials: JA
authors.lastname: García-León
authors.forename: R
authors.initials: R
authors.lastname: Maldonado-Sanchez
authors.forename: M
authors.initials: M
authors.lastname: Suardíaz
authors.forename: M
authors.initials: M
authors.lastname: Guerrero
authors.forename: G
authors.initials: G
authors.lastname: Luque
authors.forename: L
authors.initials: L
authors.lastname: Leyva
authors.forename: O
authors.initials: O
authors.lastname: Fernández
mesh_terms: D000328:Adult; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D005260:Female; D015870:Gene Expression; D006801:Humans; D016899:Interferon-beta / Q000235:genetics* / Q000276:immunology; D008297:Male; D009000:Monocytes / Q000276:immunology*; D009103:Multiple Sclerosis / Q000235:genetics* / Q000276:immunology; D053633:Receptor, Interferon alpha-beta / Q000235:genetics / Q000276:immunology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000502:physiology*
chemical_list: D053633:Receptor, Interferon alpha-beta; D016899:Interferon-beta