title: Novel multiplex oligonucleotide-conjugated bead suspension array for rapid identification of enterovirus 71 subgenogroups.
abstract: A high-throughput multiplex bead suspension array was developed for the rapid subgenogrouping of EV71 strains, based on single nucleotide polymorphisms observed within the VP1 region with a high sensitivity as low as 1 PFU. Of 33 viral isolates and 55 clinical samples, all EV71 strains were successfully detected and correctly subgenogrouped.
authors.affiliation: Department of Microbiology, Yong Loo Lin School of Medicine, National University of Singapore, 5 Science Drive 2, Kent Ridge 117597, Singapore.
authors.forename: Y
authors.initials: Y
authors.lastname: Wu
authors.forename: E L
authors.initials: EL
authors.lastname: Tan
authors.forename: A
authors.initials: A
authors.lastname: Yeo
authors.forename: K P
authors.initials: KP
authors.lastname: Chan
authors.forename: H
authors.initials: H
authors.lastname: Nishimura
authors.forename: M J
authors.initials: MJ
authors.lastname: Cardosa
authors.forename: C L
authors.initials: CL
authors.lastname: Poh
authors.forename: S H
authors.initials: SH
authors.lastname: Quak
authors.forename: Vincent T
authors.initials: VT
authors.lastname: Chow
mesh_terms: D029821:Enterovirus A, Human / Q000235:genetics* / Q000302:isolation & purification*; D004769:Enterovirus Infections / Q000175:diagnosis* / Q000821:virology; D006801:Humans; D008863:Microspheres; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA; D014773:Virology / Q000379:methods*
chemical_list: D009841:Oligonucleotides; D012367:RNA, Viral