title: Effect of protein structural integrity on cross-linking by tyrosinase evidenced by multidimensional heteronuclear magnetic resonance spectroscopy.
abstract: Enzymatic cross-linking of proteins can be catalyzed either by transferase-type enzymes, e.g., transglutaminases, or by oxidoreductases, e.g., tyrosinases or laccases. Three-dimensional structure of protein substrate plays a key role in these reactions, that is, the reactivity and end product are strongly modulated by the accessibility of target amino acid residues to the cross-linking enzyme. Typically structural integrity of protein can be distorted by heat, pH, or mechanical action, as well as by varying ionic concentration of the solution. In this study we used partially unfolded protein (wild-type DrkN SH3) and its structurally stabilized mutant (T22G) to investigate the impact of folded/unfolded conformations on cross-linking by Trichoderma reesei tyrosinase. Our results clearly showed formation of intermolecular cross-links solely between unfolded conformations, making them superior substrates to folded proteins when using tyrosinase as a cross-linking enzyme. Multidimensional heteronuclear magnetic resonance experiments in solution state were employed to investigate cross-linked end-products. The results presented in this study form basis for application development in food, medical, cosmetic, textile, packing and other sectors. In addition, the outcome of this study has a high value for the basic understanding of reaction mechanism of tyrosinases on proteins.
authors.affiliation: National Biological NMR Center, Institute of Biotechnology, P.O. Box 65, University of Helsinki, Helsinki FI-00014, Finland.
authors.forename: Maarit
authors.initials: M
authors.lastname: Hellman
authors.forename: Maija-Liisa
authors.initials: ML
authors.lastname: Mattinen
authors.forename: Biao
authors.initials: B
authors.lastname: Fu
authors.forename: Johanna
authors.initials: J
authors.lastname: Buchert
authors.forename: Perttu
authors.initials: P
authors.lastname: Permi
mesh_terms: D000596:Amino Acids / Q000737:chemistry / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000737:chemistry / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D014442:Monophenol Monooxygenase / Q000737:chemistry* / Q000378:metabolism; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D017510:Protein Folding; D011506:Proteins / Q000737:chemistry / Q000378:metabolism; D014242:Trichoderma / Q000201:enzymology; D018909:src Homology Domains
chemical_list: D000596:Amino Acids; D005656:Fungal Proteins; D011506:Proteins; D014442:Monophenol Monooxygenase