title: Co-electrospun fibrous scaffold-adsorbed DNA for substrate-mediated gene delivery.
abstract: Incorporation of gene into electrospun nanofibers for localized gene transfection of target cells represents a robust platform for tissue regeneration. In this study, a new two-step approach was explored to immobilize DNA onto electrospun nanofibers for effective gene delivery, that is, nonviral gene vector of polyethylene glycol (PEG)-modified polyethylenimine (PEI) was incorporated into scaffolds by electrospinning and then target DNA was adsorbed onto the electrospun nanofibers via electrostatic interaction between DNA and PEI-PEG. PEI-PEG/DNA particles formed from the released DNA, and PEI-PEG had a uniform particle size of approximately 200 nm. This nanofiber-based gene delivery system exhibited high transfection efficiency, in which >65% of human embryonic kidney 293 cells and >40% of mesenchymal stem cells were transfected with green fluorescent protein gene. Compared with PEI, PEG modification of PEI had improved the biocompatibility and further increased the transfection efficiency. These results suggest that the combination of nonviral gene carrier with electrospun nanofibers could be used for localized gene delivery, which has multifold potential applications in tissue engineering or as an in vivo substrate for tissue regeneration.
authors.affiliation: Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Science, Nankai University, Tianjin, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.forename: Yajun
authors.initials: Y
authors.lastname: Duan
authors.forename: Di
authors.initials: D
authors.lastname: Wei
authors.forename: Lianyong
authors.initials: L
authors.lastname: Wang
authors.forename: Hongjun
authors.initials: H
authors.lastname: Wang
authors.forename: Zhongwei
authors.initials: Z
authors.lastname: Gu
authors.forename: Deling
authors.initials: D
authors.lastname: Kong
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002478:Cells, Cultured; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D055664:Electrochemical Techniques / Q000379:methods*; D018014:Gene Transfer Techniques; D057809:HEK293 Cells; D006801:Humans; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology; D057139:Nanofibers / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry; D011094:Polyethyleneimine / Q000737:chemistry; D051381:Rats; D014162:Transfection
chemical_list: D001672:Biocompatible Materials; D011092:Polyethylene Glycols; D011094:Polyethyleneimine; D004247:DNA