title: Novel lytic bacteriophages from soil that lyse Burkholderia pseudomallei.
abstract: Burkholderia pseudomallei is a Gram-negative saprophytic bacterium that causes severe sepsis with a high mortality rate in humans and a vaccine is not available. Bacteriophages are viruses of bacteria that are ubiquitous in nature. Several lysogenic phages of Burkholderia spp. have been found but information is scarce for lytic phages. Six phages, ST2, ST7, ST70, ST79, ST88 and ST96, which lyse B. pseudomallei, were isolated from soil in an endemic area. The phages belong to the Myoviridae family. The range of estimated genome sizes is 24.0-54.6 kb. Phages ST79 and ST96 lysed 71% and 67% of tested B. pseudomallei isolates and formed plaques on Burkholderia mallei but not other tested bacteria, with the exception of closely related Burkholderia thailandensis which was lysed by ST2 and ST96 only. ST79 and ST96 were observed to clear a mid-log culture by lysis within 6 h when infected at a multiplicity of infection of 0.1. As ST79 and ST96 phages effectively lysed B. pseudomallei, their potential use as a biocontrol of B. pseudomallei in the environment or alternative treatment in infected hosts could lead to benefits from phages that are available in nature.
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
authors.forename: Umaporn
authors.initials: U
authors.lastname: Yordpratum
authors.forename: Unchalee
authors.initials: U
authors.lastname: Tattawasart
authors.forename: Surasakdi
authors.initials: S
authors.lastname: Wongratanacheewin
authors.forename: Rasana W
authors.initials: RW
authors.lastname: Sermswan
mesh_terms: D001433:Bacteriolysis; D001435:Bacteriophages / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D016957:Burkholderia pseudomallei / Q000502:physiology / Q000821:virology*; D058507:Host Specificity; D012988:Soil Microbiology