title: Adlay seed extract (Coix lachryma-jobi L.) decreased adipocyte differentiation and increased glucose uptake in 3T3-L1 cells.
abstract: The aim of the present study was to investigate effects of the ethyl acetate fraction of an ethanol extract of Coix lachryma-jobi (ECLJ) on glucose uptake and adipocyte differentiation in 3T3-L1 cells. ECLJ phosphorylated AMP-activated protein kinase (AMPK) and its downstream substrate acetyl-coenzymeA carboxylase in 3T3-L1 cells in a time- and dose-dependent manner. Moreover, we discovered that compound C inhibits ECLJ-stimulated ACC phosphorylation. In addition, ECLJ exhibited a dose-dependent stimulation of glucose uptake in 3T3-L1 cells, and this increase was obviously attenuated by compound C. ECLJ also caused a decrease in the expression levels of adipogenesis factors such as fatty acid synthase, sterol-regulatory-element-binding protein-1c, peroxisome proliferator-activated receptor γ, and CAATT/enhancer binding protein α in a dose-dependent manner. Differentiation was examined by Oil red O staining activity after ECLJ treatment for 6 days. ECLJ decreased mean droplet size. These results suggest a possible role for AMPK in the process of adipose differentiation and that ECLJ targeted for adipocyte functions could be effective in improving the symptoms of metabolic syndrome.
authors.affiliation: College of Pharmacy, Chungnam National University, Daejeon, Republic of Korea.
authors.forename: Do Thi
authors.initials: do T
authors.lastname: Ha
authors.forename: Trinh
authors.initials: T
authors.lastname: Nam Trung
authors.forename: Nguyen
authors.initials: N
authors.lastname: Bich Thu
authors.forename: Tran
authors.initials: T
authors.lastname: Van On
authors.forename: Nguyen
authors.initials: N
authors.lastname: Hai Nam
authors.forename: Chu
authors.initials: C
authors.lastname: Van Men
authors.forename: Tran
authors.initials: T
authors.lastname: Thi Phuong
authors.forename: KiHwan
authors.initials: K
authors.lastname: Bae
mesh_terms: D041721:3T3-L1 Cells; D055372:AMP-Activated Protein Kinases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D000103:Acetyl-CoA Carboxylase / Q000378:metabolism; D017667:Adipocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D050156:Adipogenesis / Q000187:drug effects*; D000818:Animals; D002934:Cinnamates / Q000032:analysis; D031742:Coix / Q000737:chemistry*; D003847:Deoxyglucose / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D005947:Glucose / Q000378:metabolism*; D006801:Humans; D051379:Mice; D009765:Obesity / Q000188:drug therapy; D010636:Phenols / Q000032:analysis; D010766:Phosphorylation / Q000187:drug effects; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D012639:Seeds / Q000737:chemistry*; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D002934:Cinnamates; D010636:Phenols; D010936:Plant Extracts; D047428:Protein Kinase Inhibitors; D003847:Deoxyglucose; D055372:AMP-Activated Protein Kinases; C526180:PRKAA1 protein, human; D000103:Acetyl-CoA Carboxylase; D005947:Glucose