title: Contribution of CmeG to antibiotic and oxidative stress resistance in Campylobacter jejuni.
abstract: OBJECTIVE
  campylobacter jejuni is a leading foodborne pathogen worldwide and its resistance to antimicrobials is a major concern for public health. The cmeG (Cj1375) gene in C. jejuni encodes a putative efflux transporter of the major facilitator family, but its function in antimicrobial resistance has not been determined. This study aimed to characterize the function of CmeG in conferring resistance to antibiotics and oxidative stress.
  
  
  METHODS
  the cmeG gene (Cj1375) in C. jejuni was inactivated by insertional mutagenesis and overexpressed by cloning with a shuttle vector. These constructs were compared with the wild-type strain using antimicrobial susceptibility tests and drug accumulation assays.
  
  
  RESULTS
  the cmeG mutation reduced bacterial growth and rendered C. jejuni more susceptible to ciprofloxacin, erythromycin, gentamicin, tetracycline, rifampicin, ethidium bromide and cholic acid as well as hydrogen peroxide, and in trans complementation restored the susceptibility to near wild-type level. RT-PCR showed that cmeG is co-transcribed with its downstream gene cmeH (Cj1376) encoding a putative periplasmic protein, but mutation of cmeH alone did not affect the susceptibility to antibiotics. Notably, overexpression of the cmeGH operon in C. jejuni NCTC 11168 significantly increased its resistance to fluoroquinolones. In addition, the cmeG mutant accumulated more EtBr and ciprofloxacin than the wild-type strain.
  
  
  CONCLUSIONS
  these results indicate that CmeG functions as a multidrug efflux transporter contributing to antibiotic resistance and oxidative defence in Campylobacter.
authors.affiliation: Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, USA.
authors.forename: Byeonghwa
authors.initials: B
authors.lastname: Jeon
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
authors.forename: Haihong
authors.initials: H
authors.lastname: Hao
authors.forename: Yi-Wen
authors.initials: YW
authors.lastname: Barton
authors.forename: Qijing
authors.initials: Q
authors.lastname: Zhang
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology* / Q000633:toxicity; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D016123:Campylobacter jejuni / Q000187:drug effects* / Q000502:physiology*; D024881:Drug Resistance, Bacterial; D004996:Ethidium / Q000633:toxicity; D017353:Gene Deletion; D015870:Gene Expression; D005822:Genetic Vectors; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D008826:Microbial Sensitivity Tests; D016254:Mutagenesis, Insertional; D018384:Oxidative Stress; D013312:Stress, Physiological
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D026901:Membrane Transport Proteins; D006861:Hydrogen Peroxide; D004996:Ethidium