title: A simple and rapid method for constructing ring-X chromosomes in Drosophila melanogaster.
abstract: Ring chromosomes are of basic interest to the geneticist and cell biologist who study their behavior in meiotic and mitotic divisions. In addition, the mitotic instability associated with some ring-X chromosomes has proven useful in Drosophila as a means to produce gynandromorphs for developmental studies. We describe a method to construct ring-X chromosomes in Drosophila via I-CreI-mediated exchange in rDNA, and then rapidly diagnose the recovery of ring chromosomes via FLP-mediated sister chromatid exchange within the ring. The method we describe provides a ready means to tailor the genetic content of ring-X chromosomes, making it suited to produce ring-X chromosomes for a variety of experimental purposes.
authors.affiliation: Department of Biology, University of Utah, Salt Lake City, UT 84112, USA.
authors.forename: Mary M
authors.initials: MM
authors.lastname: Golic
authors.forename: Kent G
authors.initials: KG
authors.lastname: Golic
mesh_terms: D000818:Animals; D059006:Chromosomes, Insect / Q000235:genetics*; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004275:DNA, Ribosomal / Q000235:genetics; D004331:Drosophila melanogaster / Q000166:cytology / Q000235:genetics* / Q000254:growth & development; D005260:Female; D005821:Genetic Techniques; D008297:Male; D008938:Mitosis; D012303:Ring Chromosomes; D012854:Sister Chromatid Exchange; D014960:X Chromosome / Q000235:genetics*
chemical_list: D004275:DNA, Ribosomal; D004262:DNA Restriction Enzymes; C048901:endodeoxyribonuclease CreI