title: Metabolomic approach with LC-QTOF to study the effect of a nutraceutical treatment on urine of diabetic rats.
abstract: The rat treated with streptozotocin has been proposed as the most appropriate model of systemic oxidative stress for studying antioxidant therapies. In that sense, rosemary extracts have long been recognized as having antioxidant properties, and folic acid may be able to improve endothelial progenitor cell function. A mixture containing both has been tested as a possible nutraceutical to improve health complications in diabetes. We have developed the methodology to evaluate metabolic changes in the urine of streptozotocin-induced diabetic rats after supplementing their diet with rosemary extract obtained with supercritical fluids (SFE) containing 10% folic acid in an acute but short-term study. It has been done with a metabolomics approach using LC-QTOF as an analytical tool. About 20 endogenous metabolites have been identified by databases and MS/MS showing statistically significant changes. Among them, several amino acids and their metabolites point to changes due to the effect of the gut microbiota. In addition, the comparison between control and streptozotocin-diabetic rats has permitted the showing of some metabolic coincidences between type 1 diabetes and other (possible) autoimmune diseases such as autism and/or Crohn's disease, and the nutraceutical intervention has succeeded in inducing changes in such biomarkers.
authors.affiliation: CEMBIO (Center for Metabolomics and Bioanalysis) Pharmacy Faculty, Campus Monteprincipe, San Pablo-CEU University, Madrid, Spain.
authors.forename: Joanna
authors.initials: J
authors.lastname: Godzien
authors.forename: Michal
authors.initials: M
authors.lastname: Ciborowski
authors.forename: Santiago
authors.initials: S
authors.lastname: Angulo
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Ruperez
authors.forename: Ma Paz
authors.initials: MP
authors.lastname: Martínez
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Señorans
authors.forename: Alejandro
authors.initials: A
authors.lastname: Cifuentes
authors.forename: Elena
authors.initials: E
authors.lastname: Ibañez
authors.forename: Coral
authors.initials: C
authors.lastname: Barbas
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy* / Q000378:metabolism / Q000652:urine*; D008297:Male; D055442:Metabolome / Q000187:drug effects; D055432:Metabolomics / Q000379:methods*; D010936:Plant Extracts / Q000494:pharmacology*; D025341:Principal Component Analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D027542:Rosmarinus / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013311:Streptozocin
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D013311:Streptozocin