title: A novel LC-MS approach for the detection of metabolites in DMPK studies.
abstract: BACKGROUND
  The profiling and quantification of drug metabolites in discovery and development bioanalysis studies is playing an increasingly important role in early candidate selection. Using a conventional tandem quadrupole mass spectrometer this activity normally requires several analytical runs to acquire the necessary analytical data.
  
  
  RESULTS
  In this article we present the use of a new tandem quadrupole mass spectrometer equipped with a novel collision cell design, which allows the rapid switching between multiple reaction monitoring and full-scan MS mode. This approach allowed for the collection of multiple reaction monitoring data and full-scan data with no loss in sensitivity, with analysis times in the 1-2 min range.
  
  
  CONCLUSIONS
  A modified approach of using the multiple reaction monitoring data to trigger the acquisition of full scan MS/MS data is described, where the data is collected on the trailing edge of the LC-MS peak, thus improving data quality and throughput.
authors.affiliation: Imperial College, Division of Surgery & Oncology, South Kensington, London, UK. r.plumb@ic.ac.uk
authors.forename: Robert S
authors.initials: RS
authors.lastname: Plumb
authors.forename: Joanne
authors.initials: J
authors.lastname: Mather
authors.forename: David
authors.initials: D
authors.lastname: Little
authors.forename: Paul D
authors.initials: PD
authors.lastname: Rainville
authors.forename: Marian
authors.initials: M
authors.lastname: Twohig
authors.forename: Gary
authors.initials: G
authors.lastname: Harland
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Kenny
authors.forename: Jeremy K
authors.initials: JK
authors.lastname: Nicholson
authors.forename: Ian D
authors.initials: ID
authors.lastname: Wilson
authors.forename: Ignatius J
authors.initials: IJ
authors.lastname: Kass
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D055808:Drug Discovery; D020719:Glucuronides / Q000032:analysis / Q000378:metabolism / Q000493:pharmacokinetics; D004364:Pharmaceutical Preparations / Q000032:analysis* / Q000378:metabolism*; D010599:Pharmacokinetics; D011433:Propranolol / Q000032:analysis / Q000378:metabolism / Q000493:pharmacokinetics; D051381:Rats; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D020719:Glucuronides; D004364:Pharmaceutical Preparations; D011433:Propranolol