title: Identification of an intracellular microdomain of the P2X7 receptor that is crucial in basolateral membrane targeting in epithelial cells.
abstract: We investigated membrane targeting of the P2X(7) receptor (P2X(7)R) in polarized epithelial cells using immunofluorescent confocal imaging. The wild-type receptor was targeted to the basolateral membrane, independently of adaptor protein μ1B. Deletion of the majority of the intracellular C-terminus, or the last 26 residues (P570-Y595), conferred targeting of the protein to the apical membrane. Alanine substitution in the microdomain P582-Q587 caused similar apical membrane targeting without major effect on the receptor function and surface expression. Our results show basolateral membrane targeting of the P2X(7)R in epithelial cells and that the intracellular C-terminal microdomain P582-Q587 is crucial in this process.
authors.affiliation: Faculty of Biological Sciences, University of Leeds, Leeds, UK.
authors.forename: H J
authors.initials: HJ
authors.lastname: Bradley
authors.forename: X
authors.initials: X
authors.lastname: Liu
authors.forename: V
authors.initials: V
authors.lastname: Collins
authors.forename: J
authors.initials: J
authors.lastname: Owide
authors.forename: G R
authors.initials: GR
authors.lastname: Goli
authors.forename: M
authors.initials: M
authors.lastname: Smith
authors.forename: A
authors.initials: A
authors.lastname: Surprenant
authors.forename: S J
authors.initials: SJ
authors.lastname: White
authors.forename: L-H
authors.initials: LH
authors.lastname: Jiang
mesh_terms: D000409:Alanine; D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000818:Animals; D002462:Cell Membrane / Q000378:metabolism*; D016764:Cell Polarity; D004285:Dogs; D004847:Epithelial Cells / Q000166:cytology* / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D042541:Intracellular Space / Q000378:metabolism*; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D021381:Protein Transport; D051381:Rats; D058486:Receptors, Purinergic P2X7 / Q000737:chemistry* / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D017384:Sequence Deletion
chemical_list: D058486:Receptors, Purinergic P2X7; D000409:Alanine