title: Characteristics of smooth muscle cells' shape and proliferation rate in novel short-term thermal angioplasty ex vivo and in vitro.
abstract: We investigated the influences on the smooth muscle cells of temporally heated arterial walls in both ex vivo and in vitro study to determine the optimum heat parameter of novel short-term thermal angioplasty, Photo-thermo Dynamic Balloon Angioplasty (PTDBA). Arterial heating dilatation was performed by the prototype PTDBA balloon ex vivo. We found that the smooth muscle cells in the vessel wall were stretch-fixed after the heating dilatation ex vivo. The stretch-fixing rate of these cells was increased with the temperature rise in the balloon of PTDBA from 60 °C to 70 °C. We measured the proliferation rate of the stretch-fixed smooth muscle cells, which were extracted from porcine arteries, on specially designed culture equipment in vitro. It was observed that the proliferation rate was inhibited at 20 % stretching compared to 10 % stretching. We think the stretch-fixing of the smooth muscle cells might not be harmful for PTDBA performances.
authors.affiliation: School of Fundamental Science and Technology, Graduate School of Science and Technology, KEIO University (Japan).
authors.forename: Mie
authors.initials: M
authors.lastname: Kunio
authors.forename: Natsumi
authors.initials: N
authors.lastname: Shimazaki
authors.forename: Arisa
authors.initials: A
authors.lastname: Ito
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Hayashi
authors.forename: Tsunenori
authors.initials: T
authors.lastname: Arai
mesh_terms: D000800:Angioplasty, Balloon; D000818:Animals; D002339:Carotid Arteries / Q000166:cytology; D049109:Cell Proliferation; D066298:In Vitro Techniques; D032389:Myocytes, Smooth Muscle / Q000166:cytology*; D013552:Swine