title: Altered expression of securin (Pttg1) and serpina3n in the auditory system of hearing-impaired Tff3-deficient mice.
abstract: BACKGROUND
  Tff3 peptide exerts important functions in cytoprotection and restitution of the gastrointestinal (GI) tract epithelia. Moreover, its presence in the rodent inner ear and involvement in the hearing process was demonstrated recently. However, its role in the auditory system still remains elusive. Our previous results showed a deterioration of hearing with age in Tff3-deficient animals.
  
  
  RESULTS
  Present detailed analysis of auditory brain stem response (ABR) measurements and immunohistochemical study of selected functional proteins indicated a normal function and phenotype of the cochlea in Tff3 mutants. However, a microarray-based screening of tissue derived from the auditory central nervous system revealed an alteration of securin (Pttg1) and serpina3n expression between wild-type and Tff3 knock-out animals. This was confirmed by qRT-PCR, immunostaining and western blots.
  
  
  CONCLUSIONS
  We found highly down-regulated Pttg1 and up-regulated serpina3n expression as a consequence of genetically deleting Tff3 in mice, indicating a potential role of these factors during the development of presbyacusis.
authors.affiliation: Division of Molecular Genetics, Institute of Human Genetics, University of Tübingen, Wilhelmstraße, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Lubka-Pathak
authors.forename: A A
authors.initials: AA
authors.lastname: Shah
authors.forename: M
authors.initials: M
authors.lastname: Gallozzi
authors.forename: M
authors.initials: M
authors.lastname: Müller
authors.forename: U
authors.initials: U
authors.lastname: Zimmermann
authors.forename: H
authors.initials: H
authors.lastname: Löwenheim
authors.forename: M
authors.initials: M
authors.lastname: Pfister
authors.forename: M
authors.initials: M
authors.lastname: Knipper
authors.forename: N
authors.initials: N
authors.lastname: Blin
authors.forename: T
authors.initials: T
authors.lastname: Schimmang
mesh_terms: D000209:Acute-Phase Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D003051:Cochlea / Q000378:metabolism; D015536:Down-Regulation; D007758:Ear, Inner / Q000378:metabolism; D016057:Evoked Potentials, Auditory, Brain Stem / Q000502:physiology; D007150:Immunohistochemistry; D051379:Mice; D018345:Mice, Knockout; D009077:Mucins / Q000172:deficiency / Q000235:genetics* / Q000378:metabolism; D010641:Phenotype; D011304:Presbycusis / Q000378:metabolism*; D064249:Securin; D015843:Serpins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D000071165:Trefoil Factor-3; D015854:Up-Regulation
chemical_list: D000209:Acute-Phase Proteins; D002352:Carrier Proteins; D009077:Mucins; D064249:Securin; C516624:Serpina3n protein, mouse; D015843:Serpins; C493263:Tff3 protein, mouse; D000071165:Trefoil Factor-3