title: Magnetic fields promote a pro-survival non-capacitative Ca2+ entry via phospholipase C signaling.
abstract: The ability of magnetic fields (MFs) to promote/increase Ca(2+) influx into cells is widely recognized, but the underlying mechanisms remain obscure. Here we analyze how static MFs of 6 mT modulates thapsigargin-induced Ca(2+) movements in non-excitable U937 monocytes, and how this relates to the anti-apoptotic effect of MFs. Magnetic fields do not affect thapsigargin-induced Ca(2+) mobilization from endoplasmic reticulum, but significantly increase the resulting Ca(2+) influx; this increase requires intracellular signal transduction actors including G protein, phospholipase C, diacylglycerol lipase and nitric oxide synthase, and behaves as a non-capacitative Ca(2+) entry (NCCE), a type of influx with an inherent signaling function, rather than a capacitative Ca(2+) entry (CCE). All treatments abrogating the extra Ca(2+) influx also abrogate the anti-apoptotic effect of MFs, demonstrating that MF-induced NCCE elicits an anti-apoptotic survival pathway.
authors.affiliation: Dipartimento di Biologia, Universita' degli Studi di Roma Tor Vergata, Via della Ricerca Scientifica snc, 00133 Roma, Italy.
authors.forename: Claudia
authors.initials: C
authors.lastname: Cerella
authors.forename: Sonia
authors.initials: S
authors.lastname: Cordisco
authors.forename: Maria Cristina
authors.initials: MC
authors.lastname: Albertini
authors.forename: Augusto
authors.initials: A
authors.lastname: Accorsi
authors.forename: Marc
authors.initials: M
authors.lastname: Diederich
authors.forename: Lina
authors.initials: L
authors.lastname: Ghibelli
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling / Q000187:drug effects / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects; D019204:GTP-Binding Proteins / Q000378:metabolism; D006801:Humans; D015544:Inositol 1,4,5-Trisphosphate / Q000096:biosynthesis; D019169:Jurkat Cells; D008071:Lipoprotein Lipase / Q000378:metabolism; D008280:Magnetics; D009569:Nitric Oxide / Q000096:biosynthesis; D019001:Nitric Oxide Synthase / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D019284:Thapsigargin / Q000494:pharmacology; D010738:Type C Phospholipases / Q000378:metabolism*; D020298:U937 Cells
chemical_list: D009569:Nitric Oxide; D019284:Thapsigargin; D015544:Inositol 1,4,5-Trisphosphate; D019001:Nitric Oxide Synthase; D008071:Lipoprotein Lipase; D010738:Type C Phospholipases; D019204:GTP-Binding Proteins; D002118:Calcium