title: Ipomoea obscura ameliorates cyclophosphamide-induced toxicity by modulating the immune system and levels of proinflammatory cytokine and GSH.
abstract: Ipomoea obscura L. is a widely used medicinal plant. The objective of this study was to investigate its protective activity against cyclophosphamide (CTX)-induced toxicity in mouse models. Swiss albino mice were treated intraperitoneally with CTX (25 mg/kg body weight) along with I. obscura extract (10 mg/kg body weight) for 10 days. Extract significantly reduced myelosuppression caused by CTX and improved the relative organ weight, total white blood cell count, and bone marrow cellularity. The elevated levels of parameters related to pathophysiology of the liver, namely glutamate pyruvate transaminase, alkaline phosphatase, and lipid peroxidation, were significantly reduced by extract treatment. Reduction of liver and intestinal glutathione levels of CTX-treated animals was reversed by I. obscura. The lowered levels of cytokines, namely IFN-γ, IL-2, and granulocyte-monocyte colony-stimulating factor after CTX treatment were found to be increased in I. obscura treated animals. Treatment with I. obscura could also decrease the level of proinflammatory cytokine TNF-α. The data suggested that I. obscura can act as a potent chemoprotective agent and can be used as an adjuvant in chemotherapeutic applications.
authors.affiliation: Department of Immunology, Amala Cancer Research Centre, Amala Nagar, Thrissur, Kerala 680 555, India.
authors.forename: T P
authors.initials: TP
authors.lastname: Hamsa
authors.forename: Girija
authors.initials: G
authors.lastname: Kuttan
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D018906:Antineoplastic Agents, Alkylating / Q000633:toxicity*; D001854:Bone Marrow Cells / Q000187:drug effects; D003520:Cyclophosphamide / Q000633:toxicity*; D016207:Cytokines / Q000097:blood*; D005978:Glutathione / Q000378:metabolism*; D007107:Immune System / Q000187:drug effects*; D027741:Ipomoea; D007583:Jejunum / Q000187:drug effects / Q000473:pathology; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008297:Male; D051379:Mice; D009929:Organ Size / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*
chemical_list: D018906:Antineoplastic Agents, Alkylating; D016207:Cytokines; D010936:Plant Extracts; D003520:Cyclophosphamide; D000469:Alkaline Phosphatase; D005978:Glutathione