title: Multiplexed and reiterative fluorescence labeling via DNA circuitry.
abstract: A class of reactive DNA circuits was adapted as erasable molecular imaging probes that allow fluorescent reporting complexes to be assembled and disassembled on a biological specimen. Circuit reactions are sequence-dependent and therefore facilitate multiplexed (multicolor) detection. Yet, the ability to disassemble reporting complexes also allows fluorophores to be removed and new circuit complexes to be used to label additional markers. Thus, these probes present opportunities to increase the total number of molecular targets that can be visualized on a biological sample by allowing multiple rounds of fluorescence microscopy to be performed.
authors.affiliation: Department of Bioengineering and Department of Chemistry, Rice University, Houston, Texas 77005, USA.
authors.forename: Dzifa Y
authors.initials: DY
authors.lastname: Duose
authors.forename: Ryan M
authors.initials: RM
authors.lastname: Schweller
authors.forename: Walter N
authors.initials: WN
authors.lastname: Hittelman
authors.forename: Michael R
authors.initials: MR
authors.lastname: Diehl
mesh_terms: D020029:Base Pairing; D015415:Biomarkers / Q000032:analysis; D004247:DNA / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D019277:Entropy; D005260:Female; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D008856:Microscopy, Fluorescence; D057054:Molecular Imaging / Q000379:methods*; D015335:Molecular Probes / Q000737:chemistry / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D013194:Staining and Labeling / Q000379:methods*; D013816:Thermodynamics
chemical_list: D015415:Biomarkers; D005456:Fluorescent Dyes; D015335:Molecular Probes; D004247:DNA