title: PUFA-dependent alteration of oxidative parameters of a canine mastocytoma cell line.
abstract: Mast cells play a key role in the immune response. Thereby, the balance of oxidative metabolism is of importance in mast cell mediator synthesis and release. Fatty acids may modify mast cell function in several ways. In this study, we investigated the influence of polyunsaturated fatty acids (PUFAs) on oxidative parameters of a canine mastocytoma cell line. C2 cells were cultured in media supplemented with linoleic acid, arachidonic acid, alpha-linolenic acid and eicosapentaenoic acid, respectively. Production of reactive oxygen species (ROS) as well as lipid peroxides was tested. Furthermore, stressor-induced DNA damage was measured. Exposure of the cells to PUFAs resulted in a significant increase in the synthesis of both ROS and lipid peroxides. Distinct differences between the PUFAs tested underline the impact of the unsaturation degree of fatty acids as well as the position of double bonds on mast cells.
authors.affiliation: Institute of Physiological Chemistry, Faculty of Veterinary Medicine, University of Leipzig, An den Tierkliniken 1 04103 Leipzig, Germany.
authors.forename: Sandra
authors.initials: S
authors.lastname: Schmutzler
authors.forename: Lisa
authors.initials: L
authors.lastname: Bachmann
authors.forename: Herbert
authors.initials: H
authors.lastname: Fuhrmann
authors.forename: Julia
authors.initials: J
authors.lastname: Schumann
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D004249:DNA Damage / Q000187:drug effects*; D004285:Dogs; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology*; D036341:Intercellular Signaling Peptides and Proteins; D015227:Lipid Peroxidation / Q000187:drug effects*; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism; D034801:Mastocytoma / Q000378:metabolism*; D010084:Oxidation-Reduction; D010455:Peptides / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism; D014862:Wasp Venoms / Q000494:pharmacology
chemical_list: D005231:Fatty Acids, Unsaturated; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; D017382:Reactive Oxygen Species; D014862:Wasp Venoms; C037500:mastoparan