title: Anti-allergic effects of sinomenine by inhibition of prostaglandin D₂ and leukotriene C₄ in mouse bone marrow-derived mast cells.
abstract: Sinomenine is an alkaloid compound and a prominent anti-allergic agent found in the root of the climbing plant Sinomenium acutum. However, its effects on the bone marrow-derived mast cell (BMMC) mediated allergy and inflammation mechanism remain unknown. In this study, the biological effects of sinomenine were evaluated while focusing on its effects on the allergic mediator in PMA plus A23187-stimulated BMMCs. An investigation was also conducted to determine its effects on the production of several allergic mediators including interleukin-6 (IL-6), prostaglandin D(2) (PGD(2)), leukotriene C(4) (LTC(4)), β-Hexosaminidase (β-Hex), and cyclooxygenase-2 (COX-2) protein. The results revealed that sinomenine inhibited the PMA plus A23187-induced production of IL-6, PGD(2), LTC(4), β-Hex, and COX-2 protein. Taken together, these findings indicate that sinomenine has the potential for use in the treatment of allergy.
authors.affiliation: Department of Oriental Pharmacy, College of Pharmacy and Wonkwang-Oriental Medicines Research Institute, Wonkwang University, Iksan, Jeonbuk, Republic of Korea.
authors.forename: You-Chang
authors.initials: YC
authors.lastname: Oh
authors.forename: Ok-Hwa
authors.initials: OH
authors.lastname: Kang
authors.forename: Jang-Gi
authors.initials: JG
authors.lastname: Choi
authors.forename: Obiang-Obounou
authors.initials: OO
authors.lastname: Brice
authors.forename: Young-Seob
authors.initials: YS
authors.lastname: Lee
authors.forename: Joon-Ho
authors.initials: JH
authors.lastname: Keum
authors.forename: Sung-Bae
authors.initials: SB
authors.lastname: Kim
authors.forename: Dong-Won
authors.initials: DW
authors.lastname: Shin
authors.forename: Jinyeul
authors.initials: J
authors.lastname: Ma
authors.forename: Gi-Hoon
authors.initials: GH
authors.lastname: Jeong
authors.forename: Dong-Yeul
authors.initials: DY
authors.lastname: Kwon
mesh_terms: D000818:Animals; D018926:Anti-Allergic Agents / Q000494:pharmacology*; D001854:Bone Marrow Cells / Q000187:drug effects* / Q000276:immunology; D002470:Cell Survival / Q000187:drug effects / Q000276:immunology; D002478:Cells, Cultured; D017997:Leukotriene C4 / Q000037:antagonists & inhibitors*; D008297:Male; D008407:Mast Cells / Q000187:drug effects* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009019:Morphinans / Q000494:pharmacology*; D015230:Prostaglandin D2 / Q000037:antagonists & inhibitors*
chemical_list: D018926:Anti-Allergic Agents; D009019:Morphinans; D017997:Leukotriene C4; C009271:sinomenine; D015230:Prostaglandin D2