title: Association of genetic variants of mannan-binding (MBL) lectin-2 gene, MBL levels and function in ulcerative colitis and Crohn's disease.
abstract: Ulcerative colitis and Crohn's disease are the two major forms of inflammatory bowel disease (IBD). A series of reports have hypothesized interplay of genetic and environmental factors in the pathogenesis of IBD. Polymorphism in the mannan-binding lectin-2 (MBL-2) gene is known to affect the structural assembly and function thereby predisposing subjects to various diseases. The present study was designed to evaluate effect of MBL-2 gene polymorphism on MBL levels and function in IBD patients. Genomic DNA was isolated from blood samples collected from 157 ulcerative colitis, 42 Crohn's disease and 204 control subjects. Genotyping for different polymorphic sites at exon1 of MBL-2 gene was performed by refractory mutation system-PCR and amplification followed by restriction digestion (PCR-RFLP). Serum MBL concentration and C4 deposition levels were estimated using ELISA. Mannan-binding lectin-2 genotypic variants were calculated in IBD and healthy controls. The frequency of single nucleotide polymorphisms at codon 54 was significantly higher in ulcerative colitis patients than controls (P < 0.0001). Ulcerative colitis patients with 'codon 54'-variation showed low serum MBL concentrations coupled with altered MBL function compared to controls. In conclusion, single nucleotide polymorphism in the MBL-2 gene is an important risk factor significantly affecting MBL levels and function in the development of ulcerative colitis among Indians.
authors.affiliation: Centre for Liver Research and Diagnostics, Deccan College of Medical Sciences, Hyderabad, Andhra Pradesh, India.
authors.forename: G
authors.initials: G
authors.lastname: Sivaram
authors.forename: Santosh K
authors.initials: SK
authors.lastname: Tiwari
authors.forename: Avinash
authors.initials: A
authors.lastname: Bardia
authors.forename: G
authors.initials: G
authors.lastname: Manoj
authors.forename: B
authors.initials: B
authors.lastname: Santhosh
authors.forename: R
authors.initials: R
authors.lastname: Saikant
authors.forename: Habeeb
authors.initials: H
authors.lastname: Aejaz
authors.forename: S
authors.initials: S
authors.lastname: Vishnupriya
authors.forename: Aleem A
authors.initials: AA
authors.lastname: Khan
authors.forename: Cm
authors.initials: C
authors.lastname: Habibullah
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D003093:Colitis, Ulcerative / Q000097:blood / Q000235:genetics* / Q000473:pathology; D003181:Complement C4 / Q000378:metabolism; D037582:Complement Pathway, Mannose-Binding Lectin / Q000502:physiology; D003424:Crohn Disease / Q000097:blood / Q000235:genetics* / Q000473:pathology; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007194:India; D008297:Male; D037601:Mannose-Binding Lectin / Q000097:blood / Q000235:genetics*; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors; D055815:Young Adult
chemical_list: D003181:Complement C4; C445619:MBL2 protein, human; D037601:Mannose-Binding Lectin