title: Biologic responses of bacteria communities living at the mucus secretion of common carp (Cyprinus carpio) after exposure to the carbon nanomaterial fullerene (C60).
abstract: Bacteria communities living in mucus secretions of common carp Cyprinus carpio (Cyprinidae) were exposed to the organic nanomaterial fullerene (C(60)) to evaluate its potential bactericidal effects. End points analyzed were viability, growth, reactive oxygen species (ROS) concentration, and total antioxidant competence against peroxyl radicals. Viability was not affected (p > 0.05), whereas growth was arrested (p < 0.05) after 3 hours of exposure to the three concentration of C(60) assayed (0.1, 1, and 10 mg/L). Levels of RO measured at different C(60) concentration showed that some colonies were reactive (significant dose-response relation, p < 0.05) to C(60), whereas others were not. The nonreactive colonies to C(60) presented higher antioxidant competence to peroxyl radicals compared with the reactive colonies (p < 0.05). The strains isolated and identified by polymerase chain reaction (PCR) products of 16S rRNA showed a predominance of Aeromonas genus between all the isolated Gram-negative bacteria. Thus, the present results indicate that C(60) affects bacterial communities that live in mucus secretions of common carp.
authors.affiliation: Programa de Pós-Graduação em Ciências Fisiológicas-Fisiologia Animal Comparada, Universidade Federal de Rio Grande, Rio Grande, Brazil.
authors.forename: Rafaela Elias
authors.initials: RE
authors.lastname: Letts
authors.forename: Talita C B
authors.initials: TC
authors.lastname: Pereira
authors.forename: Mauricio Reis
authors.initials: MR
authors.lastname: Bogo
authors.forename: José M
authors.initials: JM
authors.lastname: Monserrat
mesh_terms: D000333:Aeromonas / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000975:Antioxidants / Q000494:pharmacology*; D002347:Carps / Q000378:metabolism / Q000382:microbiology*; D004781:Environmental Exposure / Q000032:analysis*; D037741:Fullerenes / Q000737:chemistry / Q000494:pharmacology*; D009093:Mucus / Q000378:metabolism / Q000382:microbiology*; D049329:Nanostructures / Q000737:chemistry*; D010545:Peroxides / Q000378:metabolism; D016133:Polymerase Chain Reaction
chemical_list: D000900:Anti-Bacterial Agents; D000975:Antioxidants; D037741:Fullerenes; D010545:Peroxides; C049375:perhydroxyl radical