title: Identifying LRRC16B as an oncofetal gene with transforming enhancing capability using a combined bioinformatics and experimental approach.
abstract: Oncofetal genes are expressed in embryos or fetuses, are downregulated or undetectable in adult tissues, and then re-expressed in tumors. Known oncofetal genes, such as AFP, GCB, FGF18, IMP-1 and SOX1, often have important clinical applications or pivotal biological functions. To find new oncofetal-like genes, we used the public information of expressed sequence tags to systematically analyze gene expression patterns and identified a novel oncofetal-like gene, LRRC16B. It increased the proliferation, anchorage-independent growth and tumorigenesis of transformed cells in xenografts, possibly through its effects on cyclin B1 protein levels. These findings exemplify the feasibility of using bioinformatics to find new oncofetal-like genes and suggest that more genes with important functional roles will be uncovered in the candidate gene list.
authors.affiliation: Institute of Basic Medical Science, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
authors.forename: C-C
authors.initials: CC
authors.lastname: Hsu
authors.forename: C-W
authors.initials: CW
authors.lastname: Chiang
authors.forename: H-C
authors.initials: HC
authors.lastname: Cheng
authors.forename: W-T
authors.initials: WT
authors.lastname: Chang
authors.forename: C-Y
authors.initials: CY
authors.lastname: Chou
authors.forename: H-W
authors.initials: HW
authors.lastname: Tsai
authors.forename: C-T
authors.initials: CT
authors.lastname: Lee
authors.forename: Z-H
authors.initials: ZH
authors.lastname: Wu
authors.forename: T-Y
authors.initials: TY
authors.lastname: Lee
authors.forename: A
authors.initials: A
authors.lastname: Chao
authors.forename: N-H
authors.initials: NH
authors.lastname: Chow
authors.forename: C-L
authors.initials: CL
authors.lastname: Ho
mesh_terms: D000818:Animals; D000951:Antigens, Neoplasm / Q000235:genetics*; D002352:Carrier Proteins; D002460:Cell Line; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D019295:Computational Biology / Q000379:methods; D006224:Cricetinae; D056744:Cyclin B1 / Q000378:metabolism; D030541:Databases, Genetic; D020224:Expressed Sequence Tags; D005260:Female; D006801:Humans; D051379:Mice; D016688:Mice, Inbred NOD; D008840:Microfilament Proteins; D023041:Xenograft Model Antitumor Assays
chemical_list: D000951:Antigens, Neoplasm; C547525:CARMIL1 protein, human; D002352:Carrier Proteins; D056744:Cyclin B1; D008840:Microfilament Proteins; C031192:oncofetal antigens