title: Blood cell capture on antibody microarrays and monitoring of the cell capture using surface plasmon resonance imaging.
abstract: Blood is a tremendous source of data for diagnostic purposes. Thanks to the qualitative and quantitative analysis of the different cell types carried into the blood stream. To that end, cell capture of several cell types at different locations on a microarray is an interesting alternative to classical techniques run 'in solution' as it allows simultaneous characterization of several cells on a single device. In this chapter, we have described a method based on the production of antibody microarrays specific to two different cell types: B and T lymphocytes. We have also described the real-time monitoring of the cell capture on the microarray using surface plasmon resonance imaging (SPRi).
authors.affiliation: CREAB Group, SPrAM-UMR 5819 (CEA-CNRS-UJF), INAC/CEA-Grenoble, Grenoble, France. yoann.roupioz@cea.fr
authors.forename: Yoann
authors.initials: Y
authors.lastname: Roupioz
authors.forename: Sarah
authors.initials: S
authors.lastname: Milgram
authors.forename: André
authors.initials: A
authors.lastname: Roget
authors.forename: Thierry
authors.initials: T
authors.lastname: Livache
mesh_terms: D000818:Animals; D000906:Antibodies / Q000032:analysis*; D001402:B-Lymphocytes / Q000737:chemistry; D001773:Blood Cells / Q000737:chemistry*; D002460:Cell Line; D020099:Coated Materials, Biocompatible; D019084:Fluorescent Antibody Technique, Indirect; D006825:Hybridomas / Q000166:cytology; D007074:Immunoglobulin G / Q000276:immunology; D051379:Mice; D046228:Microarray Analysis / Q000295:instrumentation / Q000379:methods*; D044085:Microfluidics / Q000379:methods; D011758:Pyrroles / Q000737:chemistry; D012680:Sensitivity and Specificity; D020349:Surface Plasmon Resonance / Q000295:instrumentation / Q000379:methods*; D013601:T-Lymphocytes / Q000737:chemistry
chemical_list: D000906:Antibodies; D020099:Coated Materials, Biocompatible; D007074:Immunoglobulin G; D011758:Pyrroles