title: Planck-Benzinger thermal work function: thermodynamic characterization of the carboxy-terminus of p53 peptide fragments.
abstract: The thermodynamic parameters for six p53 carboxy-terminus peptide fragments as determined by analytical ultracentrifugal analysis were compared over the experimental temperature range of 275-310 K to evaluate the Gibbs free energy change as a function of temperature, ΔG°(T), from 0 to 400 K using our general linear third-order fitting function, ΔG°(T) = α + βT² + γT³. Data obtained at the typical experimental temperature range are not sufficient to accurately describe the variations observed in the oligomerization of these p53 fragments. It is necessary to determine a number of thermodynamic parameters, all of which can be precisely assessed using this general third-order linear fitting function. These are the heat of reaction, innate temperature-invariant enthalpy, compensatory temperatures and the thermodynamic molecular switch occurring at the thermal set point. This methodology can be used to distinguish the characteristic structure and stability of p53 carboxy-terminal fragments or other p53 mutants. It should be used for the thermodynamic characterization of any interacting biological system.
authors.affiliation: Department of Biochemistry and Molecular Biology, College of Medicine, University of Florida, Gainesville, FL 32610-0245, USA. pwchun@ufl.edu
authors.forename: Paul W
authors.initials: PW
authors.lastname: Chun
authors.forename: Marc S
authors.initials: MS
authors.lastname: Lewis
mesh_terms: D000595:Amino Acid Sequence / Q000235:genetics; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D004735:Energy Transfer; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008432:Mathematical Computing; D056004:Molecular Dynamics Simulation; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000138:chemical synthesis* / Q000235:genetics* / Q000378:metabolism; D054730:Protein Interaction Domains and Motifs / Q000235:genetics; D012996:Solutions; D013329:Structure-Activity Relationship; D013696:Temperature; D013816:Thermodynamics; D016159:Tumor Suppressor Protein p53 / Q000737:chemistry*; D014461:Ultracentrifugation
chemical_list: D004268:DNA-Binding Proteins; D010446:Peptide Fragments; D012996:Solutions; D016159:Tumor Suppressor Protein p53