title: Helicobacter pylori proinflammatory protein up-regulates NF-kappaB as a cell-translocating Ser/Thr kinase.
abstract: There has been considerable interest in virulence genes in the plasticity region of Helicobacter pylori, but little is known about many of these genes. JHP940, one of the virulence factors encoded by the plasticity region of H. pylori strain J99, is a proinflammatory protein that induces tumor necrosis factor-alpha and interleukin-8 secretion as well as enhanced translocation of NF-κB in cultured macrophages. Here we have characterized the structure and function of JHP940 to provide the framework for better understanding its role in inflammation by H. pylori. Our work demonstrates that JHP940 is the first example of a eukaryotic-type Ser/Thr kinase from H. pylori. We show that JHP940 is catalytically active as a protein kinase and translocates into cultured human cells. Furthermore, the kinase activity is indispensable for indirectly up-regulating phosphorylation of NF-κB p65 at Ser276. Our results, taken together, contribute significantly to understanding the molecular basis of the role of JHP940 in inflammation and subsequent pathogenesis caused by H. pylori. We propose to rename the jhp940 gene as ctkA (cell translocating kinase A).
authors.affiliation: Department of Chemistry, College of Natural Sciences, Seoul National University, Seoul 151-747, Korea.
authors.forename: Do Jin
authors.initials: DJ
authors.lastname: Kim
authors.forename: Kang-Seo
authors.initials: KS
authors.lastname: Park
authors.forename: Jung-Ho
authors.initials: JH
authors.lastname: Kim
authors.forename: Sang-Hwa
authors.initials: SH
authors.lastname: Yang
authors.forename: Ji Young
authors.initials: JY
authors.lastname: Yoon
authors.forename: Byeong-Gu
authors.initials: BG
authors.lastname: Han
authors.forename: Hyoun Sook
authors.initials: HS
authors.lastname: Kim
authors.forename: Sang Jae
authors.initials: SJ
authors.lastname: Lee
authors.forename: Jun Young
authors.initials: JY
authors.lastname: Jang
authors.forename: Kyoung Hoon
authors.initials: KH
authors.lastname: Kim
authors.forename: Mi Jung
authors.initials: MJ
authors.lastname: Kim
authors.forename: Jin-Su
authors.initials: JS
authors.lastname: Song
authors.forename: Hie-Joon
authors.initials: HJ
authors.lastname: Kim
authors.forename: Chung-Mo
authors.initials: CM
authors.lastname: Park
authors.forename: Sang-Kyou
authors.initials: SK
authors.lastname: Lee
authors.forename: Byung Il
authors.initials: BI
authors.lastname: Lee
authors.forename: Se Won
authors.initials: SW
authors.lastname: Suh
mesh_terms: D000244:Adenosine Diphosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000031:analogs & derivatives / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D020134:Catalytic Domain; D045744:Cell Line, Tumor; D016480:Helicobacter pylori / Q000201:enzymology* / Q000235:genetics / Q000472:pathogenicity; D006801:Humans; D007249:Inflammation / Q000378:metabolism / Q000382:microbiology; D008958:Models, Molecular; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D037521:Virulence Factors / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D016328:NF-kappa B; D037521:Virulence Factors; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; C558121:CtkA protein, Helicobacter pylori; D017346:Protein Serine-Threonine Kinases