title: Quantitative analysis of organelle abundance, morphology and dynamics.
abstract: Recent data indicate that morphological characteristics of cell organelles are important for their function in the cell. These characteristics include not only their shape, number and size, but also their distribution in the cell. Moreover, the dynamics of processes that result in changes in these characteristics (e.g. organelle fission, fusion, autophagy, transport) influence the function of the cell. For a better understanding of these processes quantitative approaches are important. Here we give an overview of contemporary biochemical and microscopy methods that are used to quantify organelle abundance, morphology and the kinetics of the processes that cause changes in these properties.
authors.affiliation: Molecular Cell Biology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), Kluyver Centre for Genomics of Industrial Fermentation, University of Groningen, Haren, The Netherlands.
authors.forename: Tim
authors.initials: T
authors.lastname: van Zutphen
authors.forename: Ida J
authors.initials: IJ
authors.lastname: van der Klei
mesh_terms: D000818:Animals; D001343:Autophagy; D001692:Biological Transport / Q000502:physiology; D002451:Cell Compartmentation / Q000502:physiology; D002459:Cell Fusion; D002477:Cells / Q000378:metabolism / Q000648:ultrastructure*; D007700:Kinetics; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D015335:Molecular Probes / Q000032:analysis; D053140:Organelle Shape; D015388:Organelles / Q000378:metabolism / Q000648:ultrastructure*; D012441:Saccharomyces cerevisiae; D013223:Statistics as Topic / Q000379:methods*
chemical_list: D015335:Molecular Probes