title: 14-3-3 proteins fine-tune plant nutrient metabolism.
abstract: 14-3-3 Proteins regulate many cellular processes by binding to phosphorylated proteins. Previous findings suggest a connection between three 14-3-3 isoforms and plant nutrient signaling. To better understand how these 14-3-3s regulate metabolism in response to changes in plant nutrient status, putative new targets involved in nitrogen (N) and sulfur (S) metabolisms have been identified. The interactions between these 14-3-3s and multiple proteins involved in N and S metabolism and altered activity of the target proteins were confirmed in planta. Using a combination of methods, this work elucidates how 14-3-3s function as modulators of plant N and S metabolic pathways.
authors.affiliation: RIKEN Plant Science Center, Yokohama, Kanagawa, Japan. rshin@psc.riken.jp
authors.forename: Ryoung
authors.initials: R
authors.lastname: Shin
authors.forename: Joseph M
authors.initials: JM
authors.lastname: Jez
authors.forename: Amarjit
authors.initials: A
authors.lastname: Basra
authors.forename: Bei
authors.initials: B
authors.lastname: Zhang
authors.forename: Daniel P
authors.initials: DP
authors.lastname: Schachtman
mesh_terms: D048948:14-3-3 Proteins / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D018527:Hydroponics; D047468:Immunoprecipitation; D053858:Metabolic Networks and Pathways; D008969:Molecular Sequence Data; D009154:Mutation; D009584:Nitrogen / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism; D030821:Plants, Genetically Modified; D011188:Potassium / Q000378:metabolism; D011485:Protein Binding; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D034622:RNA Interference; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013455:Sulfur / Q000378:metabolism
chemical_list: D048948:14-3-3 Proteins; C556234:14-3-3chi protein, Arabidopsis; C556235:14-3-3kappa protein, Arabidopsis; D029681:Arabidopsis Proteins; D010710:Phosphates; D020033:Protein Isoforms; D013455:Sulfur; D009584:Nitrogen; D011188:Potassium