title: Determination of aristolochic acid in urine using hollow fiber liquid-phase microextraction combined with high-performance liquid chromatography.
abstract: A simple and efficient hollow fiber liquid-phase microextraction (HF-LPME) technique in conjunction with high-performance liquid chromatography is presented for extraction and quantitative determination of aristolochic acid I in human urine samples. Several parameters influencing the efficiency of HF-LPME were investigated and optimized, including extraction solvent, stirring rate, extraction time, pH of donor phase and acceptor phase. Excellent sample clean-up was observed and good linearity with coefficient of 0.9999 was obtained in the range of 15.4-960 µg/L. This method provided a 230-fold enrichment factor and good repeatability with relative standard deviations (RSD) lower than 6.0%. The limit of detection value for the analyte in urine sample was 0.01 µg/L at a signal-to-noise ratio of 3. The extraction recovery from urine samples was 61.8% with an RSD of 9.71%.
authors.affiliation: Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, People's Republic of China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.forename: Juan
authors.initials: J
authors.lastname: Chen
authors.forename: Yan-Ping
authors.initials: YP
authors.lastname: Shi
mesh_terms: D034341:Aristolochic Acids / Q000302:isolation & purification / Q000652:urine*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D052617:Solid Phase Microextraction / Q000295:instrumentation / Q000379:methods*
chemical_list: D034341:Aristolochic Acids; C000228:aristolochic acid I