title: Arsenate exposure affects amino acids, mineral nutrient status and antioxidants in rice (Oryza sativa L.) genotypes.
abstract: Simulated pot experiments were conducted on four rice (Oryza sativa L.) genotypes (Triguna, IR-36, PNR-519, and IET-4786) to examine the effects of As(V) on amino acids and mineral nutrient status in grain along with antioxidant response to arsenic exposure. Rice genotypes responded differentially to As(V) exposure in terms of amino acids and antioxidant profiles. Total amino acid content in grains of all rice genotypes was positively correlated with arsenic accumulation. While, most of the essential amino acids increased in all cultivars except IR-36, glutamic acid and glycine increased in IET-4786 and PNR-519. The level of nonprotein thiols (NPTs) and the activities of superoxide dismutase (SOD; EC 1.15.1.1), glutathione reductase (GR; EC 1.6.4.2) and ascorbate peroxidase (APX; EC 1.11.1.11) increased in all rice cultivars except IET-4786. A significant genotypic variation was also observed in specific arsenic uptake (SAU; mg kg(-1)dw), which was in the order of Triguna (134) > IR-36 (71) > PNR-519 (53) > IET-4786 (29). Further, application of As(V) at lower doses (4 and 8 mg L(-1) As) enhanced the accumulation of selenium (Se) and other nutrients (Fe, P, Zn, and S), however, higher dose (12 mg L(-1) As) limits the nutrient uptake in rice. In conclusion, low As accumulating genotype, IET-4786, which also had significantly induced level of essential amino acids, seems suitable for cultivation in moderately As contaminated soil and would be safe for human consumption.
authors.affiliation: National Botanical Research Institute, Council of Scientific and Industrial Research, Lucknow-226001, India.
authors.forename: S
authors.initials: S
authors.lastname: Dwivedi
authors.forename: R D
authors.initials: RD
authors.lastname: Tripathi
authors.forename: P
authors.initials: P
authors.lastname: Tripathi
authors.forename: A
authors.initials: A
authors.lastname: Kumar
authors.forename: R
authors.initials: R
authors.lastname: Dave
authors.forename: S
authors.initials: S
authors.lastname: Mishra
authors.forename: R
authors.initials: R
authors.lastname: Singh
authors.forename: D
authors.initials: D
authors.lastname: Sharma
authors.forename: U N
authors.initials: UN
authors.lastname: Rai
authors.forename: D
authors.initials: D
authors.lastname: Chakrabarty
authors.forename: P K
authors.initials: PK
authors.lastname: Trivedi
authors.forename: B
authors.initials: B
authors.lastname: Adhikari
authors.forename: M K
authors.initials: MK
authors.lastname: Bag
authors.forename: O P
authors.initials: OP
authors.lastname: Dhankher
authors.forename: R
authors.initials: R
authors.lastname: Tuli
mesh_terms: D000596:Amino Acids / Q000378:metabolism*; D000975:Antioxidants / Q000378:metabolism*; D001149:Arsenates / Q000378:metabolism / Q000633:toxicity*; D002274:Carcinogens, Environmental / Q000378:metabolism / Q000633:toxicity*; D005838:Genotype; D015596:Nutrition Assessment; D009753:Nutritive Value; D012275:Oryza / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D012987:Soil / Q000737:chemistry; D012989:Soil Pollutants / Q000378:metabolism / Q000633:toxicity
chemical_list: D000596:Amino Acids; D000975:Antioxidants; D001149:Arsenates; D002274:Carcinogens, Environmental; D012987:Soil; D012989:Soil Pollutants; C025657:arsenic acid