title: Expression of master regulatory genes controlling skeletal development in benign cartilage and bone forming tumors.
abstract: Recent progress in skeletal molecular biology has led to the clarification of the transcriptional mechanisms of chondroblastic and osteoblastic lineage differentiation. Three master transcription factors-Sox9, Runx2, and Osterix-were shown to play an essential role in determining the skeletal progenitor cells' fate. The present study evaluates the expression of these factors in 4 types of benign bone tumors-chondromyxoid fibroma, chondroblastoma, osteoid osteoma, and osteoblastoma-using immunohistochemistry and tissue microarrays. Osteoid osteoma and osteoblastoma showed strong nuclear expression of Osterix and Runx2. In contrast, only a few chondroblastomas showed positive nuclear expression of Osterix. Strong nuclear expression of Sox9 was detected in all chondroblastomas, whereas nearly half of the osteoblastomas showed focal weak cytoplasmic expression of Sox9.
authors.affiliation: Department of Pathology, The University of Texas M. D. Anderson Cancer Center, Houston, TX 77030, USA.
authors.forename: Jane Y
authors.initials: JY
authors.lastname: Dancer
authors.forename: Stephen P
authors.initials: SP
authors.lastname: Henry
authors.forename: Jolanta
authors.initials: J
authors.lastname: Bondaruk
authors.forename: Sangkyou
authors.initials: S
authors.lastname: Lee
authors.forename: Alberto G
authors.initials: AG
authors.lastname: Ayala
authors.forename: Benoit
authors.initials: B
authors.lastname: de Crombrugghe
authors.forename: Bogdan
authors.initials: B
authors.lastname: Czerniak
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001859:Bone Neoplasms / Q000235:genetics* / Q000473:pathology; D002356:Cartilage / Q000254:growth & development* / Q000473:pathology; D002648:Child; D002804:Chondroblastoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D002812:Chondroma / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics / Q000378:metabolism; D005260:Female; D005350:Fibroma / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D005786:Gene Expression Regulation; D053263:Gene Regulatory Networks; D006801:Humans; D008297:Male; D008875:Middle Aged; D009372:Neoplasms, Connective Tissue / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D018215:Osteoblastoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D010012:Osteogenesis / Q000235:genetics*; D010017:Osteoma, Osteoid / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D055758:SOX9 Transcription Factor / Q000235:genetics / Q000378:metabolism; D000076182:Sp7 Transcription Factor; D013234:Stem Cells / Q000378:metabolism / Q000473:pathology; D046888:Tissue Array Analysis; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D055815:Young Adult
chemical_list: D050659:Core Binding Factor Alpha 1 Subunit; C493709:RUNX2 protein, human; D055758:SOX9 Transcription Factor; C527932:SOX9 protein, human; D000076182:Sp7 Transcription Factor; C478163:SP7 protein, human; D014157:Transcription Factors