title: The differentially-expressed proteome in Zn/Cd hyperaccumulator Arabis paniculata Franch. in response to Zn and Cd.
abstract: The Zn/Cd hyperaccumulator Arabis paniculata is able to tolerate high level of Zn and Cd. To clarify the molecular basis of Zn and Cd tolerance, proteomic approaches were applied to identify proteins involved in Zn and Cd stress response in A. paniculata. Plants were exposed to both low and high Zn or Cd levels for 10 d. Proteins of leaves in each treatment were separated by 2-DE (two-dimensional electrophoresis). Nineteen differentially-expressed proteins upon Zn treatments and 18 proteins upon Cd treatments were observed. Seventeen out of 19 of Zn-responsive proteins and 16 out of 18 of Cd-responsive proteins were identified using MALDI-TOF/TOF-MS (matrix-assisted laser desorption/ionization time of flight mass spectrometry). The most of identified proteins were known to function in energy metabolism, xenobiotic/antioxidant defense, cellular metabolism, protein metabolism, suggesting the responses of A. paniculata to Zn and Cd share similar pathway to certain extend. However, the different metal defense was also revealed between Zn and Cd treatment in A. paniculata. These results indicated that A. paniculata against to Zn stress mainly by enhancement of energy metabolism including auxin biosynthesis and protein metabolism to maintain plant growth and correct misfolded proteins. In the case of Cd, plants adopted antioxidative/xenobiotic defense and cellular metabolism to keep cellular redox homeostasis and metal-transportation under Cd stress.
authors.affiliation: School of Environmental Science and Engineering, Sun Yat-sen University, Guangzhou, Guangdong Province 510275, PR China; School of Public Health, Sun Yat-sen University, Guangzhou, Guangdong Province 510008, PR China.
authors.forename: Xiao-Wen
authors.initials: XW
authors.lastname: Zeng
authors.forename: Rong-Liang
authors.initials: RL
authors.lastname: Qiu
authors.forename: Rong-Rong
authors.initials: RR
authors.lastname: Ying
authors.forename: Ye-Tao
authors.initials: YT
authors.lastname: Tang
authors.forename: Lu
authors.initials: L
authors.lastname: Tang
authors.forename: Xiao-Hang
authors.initials: XH
authors.lastname: Fang
mesh_terms: D000222:Adaptation, Physiological; D031214:Arabis / Q000187:drug effects / Q000378:metabolism*; D002104:Cadmium / Q000633:toxicity*; D010940:Plant Proteins / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism; D012989:Soil Pollutants / Q000633:toxicity*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D015032:Zinc / Q000633:toxicity*
chemical_list: D010940:Plant Proteins; D020543:Proteome; D012989:Soil Pollutants; D017392:Thiobarbituric Acid Reactive Substances; D002104:Cadmium; D015032:Zinc