title: Utilization of DBS within drug discovery: development of a serial microsampling pharmacokinetic study in mice.
abstract: BACKGROUND
  Dried blood spots (DBS) are rapidly gaining a foothold in the pharmaceutical industry. However, applications in exploratory drug discovery are limited mainly owing to method development time. The development of a generic DBS assay is presented with its application in serial microsampling from mice.
  
  
  RESULTS
  A generic 'fit-for-purpose' assay was developed on FTA(®) Elute, which allowed 90% of compounds tested to reach sensitivity levels of 1 ng/ml. A ten-time point serial mouse pharmacokinetic study was conducted using 20-µl microsamples and DBS. Application of generic 'fit-for-purpose' approach did not compromise data delivery or quality.
  
  
  CONCLUSIONS
  Serial microsampling and DBS in exploratory mouse pharmacokinetic has been shown to provide superior data quality when compared with traditional plasma-based composite studies.
authors.affiliation: Department of Pharmacokinetics, Dynamics and Metabolism, Pfizer Global Research and Development, Sandwich Laboratories, Kent, UK. graeme.clark@pfizer.com
authors.forename: Graeme T
authors.initials: GT
authors.lastname: Clark
authors.forename: Julian J
authors.initials: JJ
authors.lastname: Haynes
authors.forename: Mark A J
authors.initials: MA
authors.lastname: Bayliss
authors.forename: Lisa
authors.initials: L
authors.lastname: Burrows
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000032:analysis; D001800:Blood Specimen Collection / Q000295:instrumentation / Q000379:methods* / Q000592:standards; D002138:Calibration; D003890:Desiccation; D055808:Drug Discovery / Q000379:methods*; D051379:Mice; D010209:Paper; D010599:Pharmacokinetics; D010949:Plasma / Q000737:chemistry
chemical_list: D001798:Blood Proteins