title: [Role of measuring the expression levels of a proliferation-inducing ligand and its receptors by real-time fluorescence quantitative method in children with lymphoma].
abstract: OBJECTIVE
  to investigate the role of a real-time fluorescence quantitative polymerase chain reaction (PCR) in measuring the expression levels of a proliferation-inducing ligand and its receptors expression levels in peripheral blood of children with lymphoma.
  
  
  METHODS
  real-time fluorescence quantitative PCR was used to detect the expression levels of a proliferation-inducing ligand and its receptors in patients with Hodgkin disease (n = 10) or non-Hodgkin lymphoma (n = 37) and healthy control (n = 40). The correlation between the mRNA levels of a proliferation-inducing ligand and its receptors and differential stage of malignant lymphoma was analyzed.
  
  
  RESULTS
  the results of 47 samples showed that the levels of proliferation-inducing ligand, B cell maturation antigen, transmembrane activator and CAML interactor in the peripheral blood of lymphoma in children were significantly higher than those in normal children (1.13 ± 0.09 vs 0.41 ± 0.09, 1.22 ± 0.11 vs 0.43 ± 0.10, 0.89 ± 0.12 vs 0.35 ± 0.08, all P < 0.05). The level of a proliferation-inducing ligand and its receptors had no significant difference between Hodgkin disease and non-Hodgkin lymphoma (P > 0.05). The level of a proliferation-inducing ligand in I-II stage (0.88 ± 0.06, 0.90 ± 0.08) of malignant lymphoma in children was significantly lower than that in III-IV stage (1.21 ± 0.09, 1.23 ± 0.09, P < 0.05) while the level of its receptors between various stages showed no difference (P > 0.05).
  
  
  CONCLUSIONS
  the real-time fluorescence quantitative PCR has a high sensitivity and reproducibility. A proliferation-inducing ligand may play a great role in the development and progression of malignant lymphoma in children through its receptors of B cell maturation antigen, transmembrane activator and CAML interactor. A proliferation-inducing ligand and its receptors can be established as target molecules for early diagnosis and anti-cancer therapy.
authors.affiliation: Department of Pediatric Surgery, Affiliated Hospital of Nantong University, Nantong 226007, China.
authors.forename: Jie
authors.initials: J
authors.lastname: CHEN
authors.forename: Zhi-xian
authors.initials: ZX
authors.lastname: HE
authors.forename: Dong-lei
authors.initials: DL
authors.lastname: ZHANG
authors.forename: Hua
authors.initials: H
authors.lastname: XIAN
authors.forename: Wei
authors.initials: W
authors.lastname: CAI
mesh_terms: D000293:Adolescent; D001483:Base Sequence; D016022:Case-Control Studies; D002648:Child; D005260:Female; D006689:Hodgkin Disease / Q000097:blood / Q000175:diagnosis*; D006801:Humans; D008228:Lymphoma, Non-Hodgkin / Q000097:blood / Q000175:diagnosis*; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics; D018121:Receptors, Cytokine / Q000097:blood* / Q000235:genetics; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053300:Tumor Necrosis Factor Ligand Superfamily Member 13 / Q000097:blood* / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D018121:Receptors, Cytokine; D053300:Tumor Necrosis Factor Ligand Superfamily Member 13