title: Hydrogenosome-localization of arginine deiminase in Trichomonas vaginalis.
abstract: The arginine dihydrolase (ADH) pathway has an analogous function to the urea cycle in mitochondria-containing cells, by removing nitrogen from amino acids and generating ATP. Subcellular localization of the ADH pathway enzymes in Trichomonas vaginalis revealed that arginine deiminase (ADI) localizes to the hydrogenosome, a mitochondrion-like organelle of anaerobic protists. However the other enzymes of the ADH pathway, ornithine carbamyltransferase and carbamate kinase localize to the cytosol. Three gene sequences of T. vaginalis ADI (ADI 1-3) were identified in the T. vaginalis genome, all having putative mitochondrial targeting sequences. The ADI sequences were cloned and used to probe T. vaginalis using a carboxyterminal di-hemogglutinin epitope tag which demonstrated co-localization with malic enzyme confirming the hydrogenosome localization of this enzyme.
authors.affiliation: Haskins Laboratories, Pace University, New York, NY 10038, USA.
authors.forename: Mary
authors.initials: M
authors.lastname: Morada
authors.forename: Ondrej
authors.initials: O
authors.lastname: Smid
authors.forename: Vladimir
authors.initials: V
authors.lastname: Hampl
authors.forename: Robert
authors.initials: R
authors.lastname: Sutak
authors.forename: Brian
authors.initials: B
authors.lastname: Lam
authors.forename: Paola
authors.initials: P
authors.lastname: Rappelli
authors.forename: Daniele
authors.initials: D
authors.lastname: Dessì
authors.forename: Pier L
authors.initials: PL
authors.lastname: Fiori
authors.forename: Jan
authors.initials: J
authors.lastname: Tachezy
authors.forename: Nigel
authors.initials: N
authors.lastname: Yarlett
mesh_terms: D015971:Gene Expression Regulation, Enzymologic; D006867:Hydrolases / Q000235:genetics / Q000378:metabolism*; D015388:Organelles / Q000201:enzymology; D010802:Phylogeny; D021381:Protein Transport; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D014246:Trichomonas vaginalis / Q000201:enzymology* / Q000235:genetics*
chemical_list: D011993:Recombinant Fusion Proteins; D006867:Hydrolases; C019723:arginine deiminase