title: [Study on structural characterization and purification of bioflocculant ZS-7].
abstract: The bioflocculant ZS-7 was purified to homogeneity by ethanol precipitation, dialysis and gel permeation chromatography (GPC). About 0.98 g of the purified bioflocculant could be recovered from 1L of fermentation broth. The purified bioflocculant was identified as a glycoprotein consisting of polysaccharide (91.5%) and protein (8.4%), with an approximate molecular weight of 6.89 x 10(4). The major component of ZS-7 is an acid polysaccharide including uronic (16.4%), pyruvic (7.1%) and acetic acids (0.5%). It consists of galactose, glucose, mannose and rhamnose in a molar ratio of 142 :2.2 : 4.5 : 3.4. X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared (FTIR) and NMR spectrum of the bioflocculant indicate the presence of carboxyl, hydroxyl, amide, amino, methoxyl and sulfate groups.
authors.affiliation: Institute of Water Environment and Ecological Restoration, China Water Resources Pearl River Planning Surveying and Designing Co. Ltd., Guangzhou 510610, China. lizhongshan100@yahoo.com.cn
authors.forename: Zhong
authors.initials: Z
authors.lastname: Li
authors.forename: Xiu-Yu
authors.initials: XY
authors.lastname: Zhang
authors.forename: Jian-Qiao
authors.initials: JQ
authors.lastname: Qin
authors.forename: Kai-Feng
authors.initials: KF
authors.lastname: Wang
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D005427:Flocculation; D005690:Galactose / Q000032:analysis; D006023:Glycoproteins / Q000737:chemistry* / Q000302:isolation & purification*; D008358:Mannose / Q000032:analysis*; D008970:Molecular Weight; D011134:Polysaccharides / Q000032:analysis; D011506:Proteins / Q000032:analysis; D012210:Rhamnose / Q000032:analysis*
chemical_list: D001426:Bacterial Proteins; D006023:Glycoproteins; D011134:Polysaccharides; D011506:Proteins; C554584:bioflocculant ZS-7, Bacillus licheniformis X14; D008358:Mannose; D012210:Rhamnose; D005690:Galactose