title: Restricted-access media supports for direct high-throughput analysis of biological fluid samples: review of recent applications.
abstract: This review presents an update on the use of restricted-access materials (RAMs) for direct injection of biological samples. The fundamental improvements in the preparation of tailored RAMs and the diversity of applications with these phases are presented. Insights into diminishing the matrix effect by the use of RAM supports in methods by LC-MS and into the low number of methods for enantiomeric separations by direct injections of biological samples are addressed. The diversity of systems that incorporate RAMs for selective sample clean-up or fractionation in proteome and peptidome analysis is also covered.
authors.affiliation: Departamento de Química, Cx. Postal 676, Universidade Federal de São Carlos, 13565-905, SP, Brazil.
authors.forename: Neila Maria
authors.initials: NM
authors.lastname: Cassiano
authors.forename: Juliana Cristina
authors.initials: JC
authors.lastname: Barreiro
authors.forename: Marcela Cristina
authors.initials: MC
authors.lastname: Moraes
authors.forename: Regina Vincenzi
authors.initials: RV
authors.lastname: Oliveira
authors.forename: Quezia Bezerra
authors.initials: QB
authors.lastname: Cass
mesh_terms: D000818:Animals; D001826:Body Fluids / Q000737:chemistry*; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D004364:Pharmaceutical Preparations / Q000097:blood* / Q000652:urine*; D011108:Polymers / Q000737:chemistry; D051381:Rats; D013237:Stereoisomerism; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D004364:Pharmaceutical Preparations; D011108:Polymers