title: Major antiparallel and minor parallel β sheet populations detected in the membrane-associated human immunodeficiency virus fusion peptide.
abstract: The HIV gp41 protein catalyzes fusion between viral and host cell membranes, and its apolar N-terminal region or "fusion peptide" binds to the host cell membrane and plays a key role in fusion. "HFP" is a construct containing the fusion peptide sequence, induces membrane vesicle fusion, and is an important fusion model system. Earlier solid-state nuclear magnetic resonance (SSNMR) studies showed that when HFP is associated with membranes with ∼30 mol % cholesterol, the first 16 residues have predominant β strand secondary structure and a fraction of the strands form antiparallel β sheet structure with residue 16→1/1→16 or 17→1/1→17 registries for adjacent strands. In some contrast, other SSNMR and infrared studies have been interpreted to support a large fraction of an approximately in-register parallel registry of adjacent strands. However, the samples had extensive isotopic labeling, and other structural models were also consistent with the data. This SSNMR study uses sparse labeling schemes that reduce ambiguity in the determination of the fraction of HFP molecules with parallel β registry. Quantitative analysis of the data shows that the parallel fraction is at most 0.15 with a much greater fraction of antiparallel 16→1/1→16 and 17→1/1→17 registries. These data strongly support a model of HFP-induced vesicle fusion caused by antiparallel rather than parallel registries and provide insight into the arrangement of gp41 molecules during HIV-host cell fusion. This study is an example of quantitative determination of a complex structural distribution by SSNMR, including experimentally validated inclusion of natural abundance contributions to the SSNMR data.
authors.affiliation: Department of Chemistry, Michigan State University, East Lansing, Michigan 48824, United States.
authors.forename: Scott D
authors.initials: SD
authors.lastname: Schmick
authors.forename: David P
authors.initials: DP
authors.lastname: Weliky
mesh_terms: D000595:Amino Acid Sequence; D015700:HIV Envelope Protein gp41 / Q000737:chemistry*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D010455:Peptides / Q000737:chemistry*; D017433:Protein Structure, Secondary
chemical_list: D015700:HIV Envelope Protein gp41; D010455:Peptides