title: Inhibition of tumor cell migration and invasion through knockdown of Rac1 expression in medulloblastoma cells.
abstract: Medulloblastoma is one of the common malignant brain tumors in children or young adults and its overall disease-free 5-year survival rate is approximately 50% due to tumor progression, invasion, and metastasis. This study aimed to determine whether one of Rho GTPases, Rac1 can affect the morphology, motility, and invasion of medulloblastoma cells through knockdown of Rac1 expression. Medulloblastoma Daoy cells were used to manipulate Rac1 expression using Rac1 shRNA, Rac1N17, and Rac1L61 constructs. Reverse transcriptase-PCR and western blot were used to detect expression of Rac1 mRNA and protein, respectively. Invasion and migration assays were performed to assess invasion and migration capacity of Daoy cells, respectively. The data showed that Rac1 mRNA and protein were overexpressed in Daoy cells. Deletion of Rac1 decreased the cross-linked actin network and pseudopodia and also inhibited the number of migration cells migrated or invaded to the other side of the filter compared to control cells. These data indicated that the invasion and migration in Daoy cells were inhibited by deletion of Rac1, and suggest that targeting Rac1 by Rac1 shRNA may further be evaluated and used as a potential anticancer strategy to treat medulloblastoma.
authors.affiliation: Department of Neurosurgery, Zhujiang Hospital, Southern Medical University, Guangzhou, China. baodongchen2009@yahoo.cn
authors.forename: Baodong
authors.initials: B
authors.lastname: Chen
authors.forename: Yongzhong
authors.initials: Y
authors.lastname: Gao
authors.forename: Taipeng
authors.initials: T
authors.lastname: Jiang
authors.forename: Jianjun
authors.initials: J
authors.lastname: Ding
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Zeng
authors.forename: Ruxiang
authors.initials: R
authors.lastname: Xu
authors.forename: Xiaodan
authors.initials: X
authors.lastname: Jiang
mesh_terms: D001681:Biological Assay; D045744:Cell Line, Tumor; D002465:Cell Movement; D002528:Cerebellar Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000473:pathology*; D002648:Child; D003599:Cytoskeleton / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D006801:Humans; D008527:Medulloblastoma / Q000201:enzymology* / Q000235:genetics / Q000473:pathology*; D009361:Neoplasm Invasiveness; D011554:Pseudopodia / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism; D055815:Young Adult; D020830:rac1 GTP-Binding Protein / Q000235:genetics* / Q000378:metabolism
chemical_list: C107953:RAC1 protein, human; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D020830:rac1 GTP-Binding Protein