title: Half-filter experiments for assignment, structure determination and hydration analysis of unlabelled ligands bound to 13C/15N labelled proteins.
abstract: A novel variant of the 13C/15N ω2 half-filter experiment is reported for studying the hydration of an unlabelled ligand bound to a 15N and 13C uniformly labelled biological macromolecule. This doubly tuned filter experiment represents a powerful tool for obtaining resonance assignments, structure determination and hydration properties of a ligand. Its application to the binary complex formed by the inserted-domain (I-domain) of the leukocyte function-associated antigen-1 (LFA-1) with a ligand reveals the presence of H2O molecules at the binding interface.
authors.affiliation: NOVARTIS Pharma AG, CH-4002, Basel, Switzerland.
authors.forename: C
authors.initials: C
authors.lastname: Dalvit
authors.forename: S
authors.initials: S
authors.lastname: Cottens
authors.forename: P
authors.initials: P
authors.lastname: Ramage
authors.forename: U
authors.initials: U
authors.lastname: Hommel