title: Nanocapsules incorporating IgG Fc-binding domain derived from Staphylococcus aureus protein A for displaying IgGs on immunosensor chips.
abstract: To enhance the sensitivities and antigen-binding capacities of immunosensors, oriented immobilization of antibodies on the surface of the sensor chip is critical, but to date, this has not been adequately achieved. We describe a way of adsorbing immunoglobulin (Ig) proteins onto 32-nm bio-nanocapsules (BNCs) through IgG Fc-binding domains derived from Staphylococcus aureus protein A (ZZ-BNC). This arrangement permits approximately 60 molecules of mouse total IgG bind to ZZ-BNC and all the IgG Fv regions to be displayed outwardly for the effective binding of antigens. ZZ-BNCs adsorbed onto the gold surface of the sensor chip of the quartz crystal microbalance (QCM) could markedly enhance the sensitivity and antigen-binding capacity of the chip. On the sensor chip of surface plasmon resonance (SPR), antibodies on the ZZ-BNCs showed higher affinities to each antigen than those on protein A. The BNC-coated sensor chip is very stable, and should prove useful for various immunosensor applications due to oriented immobilization of antibodies.
authors.affiliation: Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa 464-8601, Japan.
authors.forename: Masumi
authors.initials: M
authors.lastname: Iijima
authors.forename: Hiroyasu
authors.initials: H
authors.lastname: Kadoya
authors.forename: Satoko
authors.initials: S
authors.lastname: Hatahira
authors.forename: Shingo
authors.initials: S
authors.lastname: Hiramatsu
authors.forename: Giman
authors.initials: G
authors.lastname: Jung
authors.forename: Aaron
authors.initials: A
authors.lastname: Martin
authors.forename: John
authors.initials: J
authors.lastname: Quinn
authors.forename: Joohee
authors.initials: J
authors.lastname: Jung
authors.forename: Seong-Yun
authors.initials: SY
authors.lastname: Jeong
authors.forename: Eun Kyung
authors.initials: EK
authors.lastname: Choi
authors.forename: Takeshi
authors.initials: T
authors.lastname: Arakawa
authors.forename: Fumiyo
authors.initials: F
authors.lastname: Hinako
authors.forename: Masanobu
authors.initials: M
authors.lastname: Kusunoki
authors.forename: Nobuo
authors.initials: N
authors.lastname: Yoshimoto
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Niimi
authors.forename: Katsuyuki
authors.initials: K
authors.lastname: Tanizawa
authors.forename: Shun'ichi
authors.initials: S
authors.lastname: Kuroda
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D006041:Goats; D006801:Humans; D007141:Immunoglobulin Fc Fragments / Q000737:chemistry*; D007074:Immunoglobulin G / Q000737:chemistry*; D051379:Mice; D018625:Microscopy, Atomic Force; D046529:Microscopy, Electron, Transmission; D053769:Nanocapsules / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology; D011817:Rabbits; D051381:Rats; D012756:Sheep; D013205:Staphylococcal Protein A / Q000737:chemistry*; D020349:Surface Plasmon Resonance
chemical_list: D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G; D053769:Nanocapsules; D013205:Staphylococcal Protein A