title: Isolation and characteristics of the PSI-LHCI-LHCII supercomplex under high light.
abstract: We developed a novel method for the isolation of the PSI-LHCI-LHCII complex from spinach leaves. The supercomplex was resolved into a core complex (CPI), LHCII trimers, LHCI dimers and LHCII monomers using green gel electrophoresis. We then investigate changes in the fluorescence and absorption spectra of PSI-LHCI-LHCII under high light. In addition, we compared light-induced denaturation of the core protein subunits in both PSI-LHCI and PSI-LHCI-LHCII. Differences in denaturation and photochemical activity indicated that binding of LHCII increased the photosensitivity of the PSI core. Increased energy delivered to the PSI core during illumination accelerated damage to the core complex.
authors.affiliation: Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Beijing, China.
authors.forename: Xiaochun
authors.initials: X
authors.lastname: Qin
authors.forename: Wenda
authors.initials: W
authors.lastname: Wang
authors.forename: Kebing
authors.initials: K
authors.lastname: Wang
authors.forename: Yueyong
authors.initials: Y
authors.lastname: Xin
authors.forename: Tingyun
authors.initials: T
authors.lastname: Kuang
mesh_terms: D004591:Electrophoresis, Polyacrylamide Gel; D008027:Light; D045331:Photosystem I Protein Complex / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
chemical_list: D045331:Photosystem I Protein Complex