title: Microbial community fingerprinting by differential display-denaturing gradient gel electrophoresis.
abstract: Complex microbial communities exhibit a large diversity, hampering differentiation by DNA fingerprinting. Herein, differential display-denaturing gradient gel electrophoresis is proposed. By adding a nucleotide to the 3' ends of PCR primers, 16 primer pairs and fingerprints were generated per community. Complexity reduction in each partial fingerprint facilitates sample comparison.
authors.affiliation: Instituto de Recursos Naturales y Agrobiología, IRNAS-CSIC, Seville, Spain.
authors.forename: M C
authors.initials: MC
authors.lastname: Portillo
authors.forename: D
authors.initials: D
authors.lastname: Villahermosa
authors.forename: A
authors.initials: A
authors.lastname: Corzo
authors.forename: J M
authors.initials: JM
authors.lastname: Gonzalez
mesh_terms: D044822:Biodiversity; D019149:Bioreactors / Q000382:microbiology; D016000:Cluster Analysis; D016172:DNA Fingerprinting / Q000379:methods*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D058645:Denaturing Gradient Gel Electrophoresis / Q000379:methods*; D008828:Microbiological Techniques / Q000379:methods*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S