title: Transcriptome analysis of hepatocytes after partial hepatectomy in rats.
abstract: After partial hepatectomy (PH), the recovery of liver mass is mainly mediated by proliferation and enlargement of hepatocytes. Therefore, measuring the transcriptional profiling of hepatocytes after PH will be helpful in exploring the mechanism of liver regeneration (LR). Firstly, hepatocytes were isolated from rat regenerating liver at different time points following PH, and then global gene expression analysis of hepatocytes was performed using Rat Genome 230 2.0 Array. The results demonstrated that 1,417 genes in the array (including 767 known genes) were identified to be LR-related. Clustering analysis demonstrated that 767 known genes fell into six classes with distinct expression kinetics. When gene expression patterns were combined with gene functions, genes involved in acute-phase response and defense response were rapidly elevated in early phases; those in cell proliferation and DNA replication were significantly up-expressed in middle phase; a growing number of cell adhesion-involved genes were up-regulated as regeneration progressed; those in amino acid and lipid metabolism showed persistent down-regulation during LR. Based on the above analyses, it was suggested that hepatocyte defense mechanism was quickly triggered after PH; cell proliferation became active in middle phase; cell adhesion was strengthened in late phase; amino acid and lipid metabolism were attenuated during LR. Additionally, comparative analysis between transcriptional profiling of hepatocytes and regenerating liver indicated a major contribution of hepatocytes to LR.
authors.affiliation: Key Laboratory for Cell Differentiation Regulation, Henan Normal University, 46# East of Construction Road, Xinxiang, 453007, China, cellkeylab@126.com
authors.forename: Cunshuan
authors.initials: C
authors.lastname: Xu
authors.forename: Xiaoguang
authors.initials: X
authors.lastname: Chen
authors.forename: Cuifang
authors.initials: C
authors.lastname: Chang
authors.forename: Gaiping
authors.initials: G
authors.lastname: Wang
authors.forename: Wenbo
authors.initials: W
authors.lastname: Wang
authors.forename: Lianxing
authors.initials: L
authors.lastname: Zhang
authors.forename: Qiushi
authors.initials: Q
authors.lastname: Zhu
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Fuchun
authors.initials: F
authors.lastname: Zhang
mesh_terms: D000818:Animals; D020869:Gene Expression Profiling; D006498:Hepatectomy; D022781:Hepatocytes / Q000378:metabolism*; D008099:Liver / Q000166:cytology* / Q000502:physiology; D016133:Polymerase Chain Reaction; D051381:Rats; D017207:Rats, Sprague-Dawley; D012038:Regeneration