title: Improved instrumentation for large-size two-dimensional protein maps.
abstract: Novel instrumentation for performing large-size (>25 cm) 2-D maps is reported here. To perform the first dimension, we developed a power supply that can deliver a voltage of up to 15,000 V and allows regulation of current (up to 200 μA) onto each individual focusing IPG strip. The IEF strip tray can accommodate up to 12 IPG strips and the electrodes slide on a ruler, thus permitting running strips of any length up to 45 cm. In addition, this apparatus also includes a second power supply that allows the performance of electrophoresis at high amperage (400 mA) and a Peltier system that allows a 10-80°C temperature control.
authors.affiliation: Department of Clinical and Experimental Medicine, Chair of Metabolism, University of Padova, Padova, Italy. millionirenato@gmail.com
authors.forename: Renato
authors.initials: R
authors.lastname: Millioni
authors.forename: Manuela
authors.initials: M
authors.lastname: Miuzzo
authors.forename: Lucia
authors.initials: L
authors.lastname: Puricelli
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Iori
authors.forename: Stefano
authors.initials: S
authors.lastname: Sbrignadello
authors.forename: Ryan
authors.initials: R
authors.lastname: Dosselli
authors.forename: Daniela
authors.initials: D
authors.lastname: Cecconi
authors.forename: Paolo
authors.initials: P
authors.lastname: Tessari
authors.forename: Pier Giorgio
authors.initials: PG
authors.lastname: Righetti
mesh_terms: D001798:Blood Proteins / Q000737:chemistry; D011211:Electric Power Supplies; D015180:Electrophoresis, Gel, Two-Dimensional / Q000295:instrumentation* / Q000379:methods*; D006801:Humans; D007525:Isoelectric Focusing; D010449:Peptide Mapping / Q000295:instrumentation* / Q000379:methods*; D040901:Proteomics / Q000295:instrumentation* / Q000379:methods*; D013696:Temperature
chemical_list: D001798:Blood Proteins