title: Tomato necrotic ringspot virus, a new tospovirus isolated in Thailand.
abstract: A new tospovirus isolated from naturally infected tomato plants grown in Nakhon Pathom province (Thailand) was characterized. Infected plants showed symptoms consisting of necrotic spots, necrotic ringspots and stem necrosis. This virus was detected using general antibodies that could recognize watermelon silver mottle virus (WSMoV), capsicum chlorosis virus (CaCV) and melon yellow spot virus (MYSV). However, it did not react with specific monoclonal antibodies (MAbs) to WSMoV and CaCV or a specific MAb to MYSV. The complete nucleotide sequences of S and M RNAs of the virus were determined. They were 3,023 and 4,716 nucleotides in length, respectively, and contained two ORFs in an ambisense arrangement. Sequence analysis indicated that amino acid sequence of the N protein shared 58.2%, 56.0% and 51.8% identity with those of CaCV, WSMoV and MYSV, respectively. The virus was experimentally transmitted by Thrips palmi and Ceratothripoides claratris. Based on our results, we conclude that this tospovirus isolate should be considered a member of a new species. The name tomato necrotic ringspot virus (TNRV) is proposed for this tospovirus.
authors.affiliation: Department of Plant Pathology, Faculty of Agriculture, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom, Thailand.
authors.forename: Channarong
authors.initials: C
authors.lastname: Seepiban
authors.forename: Oraprapai
authors.initials: O
authors.lastname: Gajanandana
authors.forename: Tipvadee
authors.initials: T
authors.lastname: Attathom
authors.forename: Supat
authors.initials: S
authors.lastname: Attathom
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002044:Bunyaviridae Infections / Q000635:transmission / Q000821:virology; D017931:DNA Primers / Q000235:genetics; D007303:Insect Vectors / Q000821:virology; D018551:Solanum lycopersicum / Q000821:virology*; D008969:Molecular Sequence Data; D019590:Nucleocapsid Proteins / Q000235:genetics / Q000276:immunology; D016366:Open Reading Frames; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology; D012367:RNA, Viral / Q000235:genetics; D012703:Serotyping; D013785:Thailand; D017865:Tospovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D014764:Viral Proteins / Q000235:genetics
chemical_list: D017931:DNA Primers; D019590:Nucleocapsid Proteins; D012367:RNA, Viral; D014764:Viral Proteins