title: Novel genes differentially expressed between posterior and median silk gland identified by SAGE-aided transcriptome analysis.
abstract: Serial analysis of gene expression (SAGE) profiles, from posterior and median cells of the silk gland of Bombyx mori, were analyzed and compared, so as to identify their respective distinguishing functions. The annotation of the SAGE libraries was performed with a B. mori reference tag collection, which was extracted from a novel set of Bombyx ESTs, sequenced from the 3' side. Most of the tags appeared at similar relative concentration within the two libraries, and corresponded with region-specific and highly abundant silk proteins. Strikingly, in addition to tags from silk protein mRNAs, 19 abundant tags were found (≥ 0.1%), in the median cell library, which were absent in the posterior cell tag collection. With the exception of tags from SP1 mRNA, no PSG specific tags were found in this subset class. The analysis of some of the MSG-specific transcripts, suggested that middle silk gland cells have diversified functions, in addition to their well characterized role in silk sericins synthesis and secretion.
authors.affiliation: Unité Nationale Séricicole, INRA, 25 Quai Jean Jacques Rousseau, La Mulatière F-69350, France.
authors.forename: Corinne
authors.initials: C
authors.lastname: Royer
authors.forename: Jérôme
authors.initials: J
authors.lastname: Briolay
authors.forename: Annie
authors.initials: A
authors.lastname: Garel
authors.forename: Patrick
authors.initials: P
authors.lastname: Brouilly
authors.forename: Shun-ichi
authors.initials: S
authors.lastname: Sasanuma
authors.forename: Motoe
authors.initials: M
authors.lastname: Sasanuma
authors.forename: Michihiko
authors.initials: M
authors.lastname: Shimomura
authors.forename: Céline
authors.initials: C
authors.lastname: Keime
authors.forename: Olivier
authors.initials: O
authors.lastname: Gandrillon
authors.forename: Yongping
authors.initials: Y
authors.lastname: Huang
authors.forename: Gérard
authors.initials: G
authors.lastname: Chavancy
authors.forename: Kazuei
authors.initials: K
authors.lastname: Mita
authors.forename: Pierre
authors.initials: P
authors.lastname: Couble
mesh_terms: D000818:Animals; D001483:Base Sequence; D012831:Bombyx / Q000235:genetics*; D020224:Expressed Sequence Tags; D005260:Female; D005349:Fibroins / Q000235:genetics*; D015870:Gene Expression; D020869:Gene Expression Profiling; D015723:Gene Library; D017344:Genes, Insect; D008969:Molecular Sequence Data; D005810:Multigene Family; D012333:RNA, Messenger; D017421:Sequence Analysis; D047030:Sericins / Q000235:genetics*
chemical_list: D012333:RNA, Messenger; D047030:Sericins; D005349:Fibroins