title: Qualitative and quantitative detection of agricultural microorganisms expressing iturin and mop cyclase in soils.
abstract: The environmental release of genetically engineered microorganisms (GEMs) to improve agriculture or remediate environmental hazards has raised concern over the fate of the organisms and their engineered genes. To detect the microorganisms released into the environment at the molecular level, Bacillus subtilis KB producing iturin and Pseudomonas fluorescens MX1 carrying the moc (mannityl opine catabolism) region from the Agrobacterium tumefaciens were employed as model microorganisms. Using specific fusion primers and the TaqMan probes, qualitative and quantitative detections of the model organisms by PCR and real-time PCR were conducted employing a small-scale soil-core device and pots during the six month period. The data indicate that the model bacteria can be easily detected by qualitative and quantitative methods in the test systems employed, and they do not give significant impacts on the other bacteria in soils on the Southern blotting analysis, although long-term observation may be needed.
authors.affiliation: Division of Biosystems Research, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Korea.
authors.forename: Sung Eun
authors.initials: SE
authors.lastname: Kim
authors.forename: Jae Sun
authors.initials: JS
authors.lastname: Moon
authors.forename: Won Sik
authors.initials: WS
authors.lastname: Choi
authors.forename: Eun Na
authors.initials: EN
authors.lastname: Lee
authors.forename: Sang Han
authors.initials: SH
authors.lastname: Lee
authors.forename: Sung Uk
authors.initials: SU
authors.lastname: Kim
mesh_terms: D016960:Agrobacterium tumefaciens / Q000201:enzymology / Q000235:genetics; D001412:Bacillus subtilis / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D005818:Genetic Engineering; D008969:Molecular Sequence Data; D011551:Pseudomonas fluorescens / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D012987:Soil / Q000032:analysis*; D012988:Soil Microbiology
chemical_list: D001426:Bacterial Proteins; D012987:Soil