title: DNA binding and gel electrophoresis studies of a new silver(I) complex containing 2,9-dimethyl-1,10-phenanthroline ligands.
abstract: The silver(I) complex, [Ag(2,9-dimethyl-1,10-phenanthroline)(2)](NO(3)) · H(2)O, has been synthesized and characterized by physicochemical and spectroscopic methods. The binding interactions of this complex with calf thymus DNA (CT-DNA) were investigated using emission, absorption, circular dichroism, viscosity measurements, and gel electrophoresis studies. The calculated binding constant, K(b), obtained from UV-vis absorption studies was 5.3 ± 0.2 × 10(4) M(-1). In fluorimetric studies, the enthalpy and entropy of the reaction between the complex and CT-DNA showed hydrophobic interaction. In addition, in the circular dichroism spectrum, silver(I) complex induces a B → A structural transition of CT-DNA. Gel electrophoresis studies demonstrated that this complex has ability to cleave the supercoiled plasmid DNA. All these results suggest that the complex interacts with CT-DNA via partial intercalative mode of binding.
authors.affiliation: Faculty of Chemistry, Razi University, Kermanshah, Iran. nahidshahabadi@yahoo.com
authors.forename: Nahid
authors.initials: N
authors.lastname: Shahabadi
authors.forename: Soheila
authors.initials: S
authors.lastname: Kashanian
authors.forename: Zeinab
authors.initials: Z
authors.lastname: Ahmadipour
mesh_terms: D000818:Animals; D001667:Binding, Competitive; D002417:Cattle; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D007700:Kinetics; D015394:Molecular Structure; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D010618:Phenanthrolines / Q000737:chemistry* / Q000378:metabolism; D012834:Silver / Q000737:chemistry* / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013816:Thermodynamics; D014783:Viscosity
chemical_list: D009942:Organometallic Compounds; D010618:Phenanthrolines; D012834:Silver; C002701:neocuproine; D004247:DNA; C427203:calf thymus DNA