title: Stat3 is essential for neuronal differentiation through direct transcriptional regulation of the Sox6 gene.
abstract: The transcription factor Signal Transducer and Activator of Transcription 3 (Stat3) functions in various cellular processes including neuronal differentiation. We show that the SRY-box containing gene 6 (Sox6) gene, important for neuronal differentiation, is a direct target gene of Stat3. We demonstrate that in response to ligand stimulation, Stat3 binds to the Sox6 promoter and induces its expression. Furthermore, Stat3 is activated and Sox6 is induced during neuronal differentiation of P19 cells in the absence of exogenous ligand treatment. Moreover, using an RNA interference approach, we show that Stat3 is required for Sox6 expression during neuronal differentiation.
authors.affiliation: Department of Physiology and Biophysics, Weill Medical College of Cornell University, New York, NY 10065, USA.
authors.forename: Marylynn
authors.initials: M
authors.lastname: Snyder
authors.forename: Xin-Yun
authors.initials: XY
authors.lastname: Huang
authors.forename: J Jillian
authors.initials: JJ
authors.lastname: Zhang
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D053605:Leukemia Inhibitory Factor / Q000494:pharmacology; D051379:Mice; D041681:NIH 3T3 Cells; D009474:Neurons / Q000378:metabolism / Q000473:pathology; D053683:Oncostatin M / Q000494:pharmacology; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding; D034622:RNA Interference; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055755:SOXD Transcription Factors / Q000235:genetics*; D050796:STAT3 Transcription Factor / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic / Q000235:genetics*
chemical_list: D053605:Leukemia Inhibitory Factor; D055755:SOXD Transcription Factors; D050796:STAT3 Transcription Factor; C095939:Sox6 protein, mouse; D053683:Oncostatin M