title: Effect of shock wave treatment on platelet-rich plasma added to osteoblast cultures.
abstract: The aim of this study was to verify the effects on osteoblast cultures of adding a platelet-rich plasma (PRP) concentrate pretreated with 500 shock wave (SW) at an energy flow density of 0.17 mJ/mm(2), emitted by an electromagnetic generator Minilith SL1 (STORZ, Germany), reproducing the conditions of our previous study in which we apply SW directly on osteoblasts. Real-time PCR showed that in osteoblast cultures with added PRP pretreated with SW, there was an increased expression at 48 h of insulin-like growth factor binding protein 3 (IGFBP-3) and runt-related transcription factor 2 (RUNX2) and at 72 h, of collagen type I, osteocalcin, insulin-like growth factor 1 (IGF-1) as well as IGFBP-3. Western blotting confirmed the increased protein synthesis of IGFBP-3. This experience suggests that extracorporeal shock wave treatment (ESWT) should stimulate osteogenesis also by indirect platelets-mediated network. It therefore seems possible that combining the two methods, ESWT and bioengineering procedures to infiltrate PRP and growth factors, could be a successful approach.
authors.affiliation: Department of Clinical Methodology and Surgical Techniques, Orthopedics Section, Faculty of Medicine and Surgery of University of Bari, General Hospital, Bari, Italy. angelanotarnicola@yahoo.it
authors.forename: Angela
authors.initials: A
authors.lastname: Notarnicola
authors.forename: Roberto
authors.initials: R
authors.lastname: Tamma
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Moretti
authors.forename: Antonio
authors.initials: A
authors.lastname: Panella
authors.forename: Stefania
authors.initials: S
authors.lastname: Dell'endice
authors.forename: Alberta
authors.initials: A
authors.lastname: Zallone
authors.forename: Biagio
authors.initials: B
authors.lastname: Moretti
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002478:Cells, Cultured; D050659:Core Binding Factor Alpha 1 Subunit / Q000378:metabolism; D057086:High-Intensity Focused Ultrasound Ablation; D018972:Insulin-Like Growth Factor Binding Protein 3 / Q000378:metabolism; D007334:Insulin-Like Growth Factor I / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D010006:Osteoblasts / Q000378:metabolism*; D053657:Platelet-Rich Plasma / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D050659:Core Binding Factor Alpha 1 Subunit; D018972:Insulin-Like Growth Factor Binding Protein 3; C493716:Runx2 protein, mouse; D007334:Insulin-Like Growth Factor I