title: Bisulfite-free analysis of 5MeC-binding proteins and locus-specific methylation density using a microparticle-based flow cytometry assay.
abstract: DNA methylation analysis is emerging as a new technique with potential capabilities for early cancer detection. However, current state-of-the-art techniques are not easily translatable into routine clinical methods. Herein we describe a bead-based flow cytometry assay which combines DNA hybridization to microparticles with 5MeC-specific proteins/antibodies. These assays can be used to study the binding properties of current and emerging 5MeC-binding proteins and may also have potential in the measurement of 5MeC density in clinical samples for cancer detection.
authors.affiliation: The University of Queensland, Australian Institute for Bioengineering and Nanotechnology, Centre for Biomarker Research and Development, St Lucia, Brisbane, Australia.
authors.forename: Simon R
authors.initials: SR
authors.lastname: Corrie
authors.forename: Pavel
authors.initials: P
authors.lastname: Sova
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Feng
authors.forename: Tiffany
authors.initials: T
authors.lastname: Blair
authors.forename: Nancy B
authors.initials: NB
authors.lastname: Kiviat
authors.forename: Matt
authors.initials: M
authors.lastname: Trau
mesh_terms: D044503:5-Methylcytosine / Q000378:metabolism*; D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000235:genetics / Q000378:metabolism; D019175:DNA Methylation; D004268:DNA-Binding Proteins / Q000032:analysis* / Q000378:metabolism; D005434:Flow Cytometry / Q000379:methods*; D056426:Genetic Loci / Q000235:genetics*; D006801:Humans; D008863:Microspheres; D009693:Nucleic Acid Hybridization; D013447:Sulfites
chemical_list: D004268:DNA-Binding Proteins; D013447:Sulfites; D044503:5-Methylcytosine; D004247:DNA; C042345:hydrogen sulfite