title: α-Lipoic acid has anti-inflammatory and anti-oxidative properties: an experimental study in rats with carrageenan-induced acute and cotton pellet-induced chronic inflammations.
abstract: α-Lipoic acid (ALA) has been termed the 'ideal' antioxidant, a readily absorbed and bioavailable compound capable of scavenging a number of free radicals, and it has been used for treating diseases in which oxidative stress plays a major role. The present study was designed to gain a better understanding for the positive effects of ALA on the models of acute and chronic inflammation in rats, and also determine its anti-oxidative potency. In an acute model, three doses of ALA (50, 100 and 200 mg/kg) and one dose of indomethacin (25 mg/kg) or diclofenac (25 mg/kg) were administered to rats by oral administration. The paw volumes of the animals were calculated plethysmometrically, and 0·1 ml of 1 % carrageenan (CAR) was injected into the hind paw of each animal 1 h after oral drug administration. The change in paw volume was detected as five replicates every 60 min by plethysmometry. In particular, we investigated the activities of catalase, superoxide dismutase (SOD), glutathione S-transferase (GST), glutathione peroxidase (GPx), glutathione reductase (GR), inducible NO synthase (iNOS) and myeloperoxidase (MPx), and the amounts of lipid peroxidation (LPO) or total GSH in the paw tissues of CAR-injected rats. We showed that ALA exhibited anti-inflammatory effects on both acute and chronic inflammations, and a strongly anti-oxidative potency on linoleic acid oxidation. Moreover, the administration of CAR induced oedema in the paws. ALA significantly inhibited the ability of CAR to induce: (1) the degree of acute inflammation, (2) the rise in MPx activity, (3) the increases of GST and iNOS activities and the amount of LPO and (4) the decreases of GPx, GR and SOD activities and the amount of GSH. In conclusion, these results suggest that the anti-inflammatory properties of ALA, which has a strong anti-oxidative potency, could be related to its positive effects on the antioxidant system in a variety of tissues in rats.
authors.affiliation: Department of Biochemistry, Faculty of Pharmacy, Ataturk University, 25240 Erzurum, Turkey. fodabasoglu@yahoo.com
authors.forename: Fehmi
authors.initials: F
authors.lastname: Odabasoglu
authors.forename: Zekai
authors.initials: Z
authors.lastname: Halici
authors.forename: Hayati
authors.initials: H
authors.lastname: Aygun
authors.forename: Mesut
authors.initials: M
authors.lastname: Halici
authors.forename: Fadime
authors.initials: F
authors.lastname: Atalay
authors.forename: Ahmet
authors.initials: A
authors.lastname: Cakir
authors.forename: Elif
authors.initials: E
authors.lastname: Cadirci
authors.forename: Yasin
authors.initials: Y
authors.lastname: Bayir
authors.forename: Halis
authors.initials: H
authors.lastname: Suleyman
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology / Q000627:therapeutic use*; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology / Q000627:therapeutic use*; D002351:Carrageenan; D032561:Cotton Fiber; D004195:Disease Models, Animal; D004487:Edema / Q000139:chemically induced / Q000517:prevention & control*; D004798:Enzymes / Q000378:metabolism*; D006614:Hindlimb; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D019787:Linoleic Acid / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D008063:Thioctic Acid / Q000494:pharmacology / Q000627:therapeutic use*
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D004798:Enzymes; D008063:Thioctic Acid; D002351:Carrageenan; D019787:Linoleic Acid