title: Rapid quantification of lipids in Acremonium chrysogenum using Oil red O.
abstract: A method based on staining condition and volume of culture broth for the rapid estimation of the level of intracellular lipids in Acremonium chrysogenum using Oil red O was developed. Lipids in A. chrysogenum were strongly stained by the modified Oil red O after treatment for 10 min at 75°C. The results of the study indicated that the Oil red O staining method developed here is useful for the quantification of 0.1-5 mg ml(-1) of lipids in A. chrysogenum.
authors.affiliation: Department of Chemical and Biological Engineering, Korea University, 1-5Ka, Anam-Dong, Sungbuk-Ku, Seoul, 136-701, Republic of Korea.
authors.forename: Hyun Yong
authors.initials: HY
authors.lastname: Shin
authors.forename: Jin Young
authors.initials: JY
authors.lastname: Lee
authors.forename: Eun Ji
authors.initials: EJ
authors.lastname: Kim
authors.forename: Seung Wook
authors.initials: SW
authors.lastname: Kim
mesh_terms: D000164:Acremonium / Q000737:chemistry*; D001391:Azo Compounds / Q000378:metabolism*; D008055:Lipids / Q000032:analysis*; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D001391:Azo Compounds; D008055:Lipids; C011049:oil red O