title: Defective adaption of erythrocytes during acute hypoxia injury in an elderly population.
abstract: The present study investigated the changes in several erythrocyte oxidative stress biomarkers in hypoxic elderly individuals to analyze the deleterious effects of low oxyhemoglobin saturation in an elderly population. We collected blood samples from one normoxic middle-aged group and two groups composed of individuals older than 75 years of age: one normoxic group and one hypoxic group. Aging appeared to provoke a defective erythrocyte antioxidant defense associated with increased oxidative damage in the elderly population. Acute hypoxia activated an insufficient antioxidant defense response as suggested by the oxidative damage observed. The oxidative imbalance presented in older participants and increased in hypoxia participants had a direct effect on glyceraldehyde-3-phosphate dehydrogenase cell distribution. Oxidative stress levels altered Band 3 protein and mediated caspase-3 activation in erythrocyte from the aged group although it was not extended to hypoxic individuals. Therefore, aged participants appeared to activate an insufficient antioxidant response against hypoxia-related oxidative stress.
authors.affiliation: Department of Morphology and Cellular Biology, Faculty of Medicine, University of Oviedo, 33006 Oviedo, Spain. ddgonzalo@hotmail.com
authors.forename: David
authors.initials: D
authors.lastname: de Gonzalo-Calvo
authors.forename: Kim
authors.initials: K
authors.lastname: Neitzert
authors.forename: María
authors.initials: M
authors.lastname: Fernández
authors.forename: Ignacio
authors.initials: I
authors.lastname: Vega-Naredo
authors.forename: Beatriz
authors.initials: B
authors.lastname: Caballero
authors.forename: Marina
authors.initials: M
authors.lastname: García-Macia
authors.forename: Francisco Manuel
authors.initials: FM
authors.lastname: Suárez
authors.forename: María Josefa
authors.initials: MJ
authors.lastname: Rodríguez-Colunga
authors.forename: Juan José
authors.initials: JJ
authors.lastname: Solano
authors.forename: Ana
authors.initials: A
authors.lastname: Coto-Montes
mesh_terms: D000222:Adaptation, Physiological; D000368:Aged; D000369:Aged, 80 and over; D001457:Anion Exchange Protein 1, Erythrocyte / Q000502:physiology; D000975:Antioxidants / Q000502:physiology; D053148:Caspase 3 / Q000502:physiology; D002374:Catalase / Q000502:physiology; D004912:Erythrocytes / Q000201:enzymology / Q000502:physiology*; D005260:Female; D005980:Glutathione Reductase / Q000502:physiology; D024602:Glyceraldehyde-3-Phosphate Dehydrogenase (NADP+)(Phosphorylating) / Q000502:physiology*; D006801:Humans; D000860:Hypoxia / Q000201:enzymology / Q000503:physiopathology*; D008297:Male; D018384:Oxidative Stress; D013482:Superoxide Dismutase / Q000502:physiology
chemical_list: D001457:Anion Exchange Protein 1, Erythrocyte; D000975:Antioxidants; D002374:Catalase; D013482:Superoxide Dismutase; D024602:Glyceraldehyde-3-Phosphate Dehydrogenase (NADP+)(Phosphorylating); D005980:Glutathione Reductase; D053148:Caspase 3