title: Robust electrochemical system for screening single nucleotide polymorphisms.
abstract: A highly sensitive and selective electrochemical DNA signaling scheme, which identifies the point mutation existing in target DNA sequence, is developed based on the combination of label-free hairpin probe (HP)/DNA endonuclease with zirconia (ZrO(2)) nanoparticle film, representing a promising screening platform for the accurate diagnosis of infections and genetic diseases as well as for environmental and forensic applications.
authors.affiliation: State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, P. R. China.
authors.forename: Rong
authors.initials: R
authors.lastname: Hu
authors.forename: Zai-Sheng
authors.initials: ZS
authors.lastname: Wu
authors.forename: Songbai
authors.initials: S
authors.lastname: Zhang
authors.forename: Guo-Li
authors.initials: GL
authors.lastname: Shen
authors.forename: Ruqin
authors.initials: R
authors.lastname: Yu
mesh_terms: D004247:DNA / Q000032:analysis*; D015342:DNA Probes / Q000737:chemistry; D055664:Electrochemical Techniques / Q000379:methods*; D055029:Inverted Repeat Sequences; D053768:Metal Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D020641:Polymorphism, Single Nucleotide; D015040:Zirconium / Q000737:chemistry
chemical_list: D015342:DNA Probes; D004247:DNA; D015040:Zirconium; C028541:zirconium oxide