title: Effects of cell entrapment on nucleic acid content and microbial diversity of mixed cultures in biological wastewater treatment.
abstract: The effects of entrapment on nucleic acid content and microbial diversity of mixed cultures in biological municipal wastewater treatment were investigated. Deoxyribonucleic acid content increased 1.6-5.5 times more in alginate entrapped cells than in free and polyvinyl alcohol (PVA) entrapped cells. PVA entrapment resulted in 1.1- to 5.9-fold more increases in ribonucleic acid content compared to that experienced by free and alginate entrapped cells. Entrapment in carrageenan changed the bacterial community structure more than the alginate and PVA entrapments (35-80% versus 0-35%) as determined by single-strand conformation polymorphism analyses. The change in the bacterial community structure of alginate entrapped cells was less time dependent than that of PVA entrapped cells. This study enhances understandings on the physiology of entrapped cells and their community evolution in wastewater treatment environments.
authors.affiliation: Department of Civil Engineering, North Dakota State University, Fargo, ND 58108, USA.
authors.forename: Sudipta
authors.initials: S
authors.lastname: Pramanik
authors.forename: John
authors.initials: J
authors.lastname: McEvoy
authors.forename: Sumana
authors.initials: S
authors.lastname: Siripattanakul
authors.forename: Eakalak
authors.initials: E
authors.lastname: Khan
mesh_terms: D001420:Bacteria, Aerobic / Q000302:isolation & purification* / Q000378:metabolism*; D001422:Bacterial Adhesion; D009696:Nucleic Acids; D013045:Species Specificity; D014871:Water Microbiology; D014874:Water Pollutants, Chemical / Q000378:metabolism*; D018508:Water Purification / Q000379:methods*
chemical_list: D009696:Nucleic Acids; D014874:Water Pollutants, Chemical