title: Adipogenic differentiation of adipose-derived stem cells.
abstract: The primary physiological function of adipose-derived stem cells (ASCs) is to differentiate into adipose tissue. It is now possible to isolate, expand, and cryopreserve ASC from adipose depots of many animal species. These ASC can be induced to undergo adipogenic differentiation in vitro by exposure to a cocktail of chemical agents or inductive growth factors. The current chapter describes methods to induce adipogenesis and to quantify this differentiation process in vitro.
authors.affiliation: Stem Cell Biology Laboratory, Pennington Biomedical Research Center, Louisiana State University System, Baton Rouge, LA, USA.
authors.forename: Gang
authors.initials: G
authors.lastname: Yu
authors.forename: Z Elizabeth
authors.initials: ZE
authors.lastname: Floyd
authors.forename: Xiying
authors.initials: X
authors.lastname: Wu
authors.forename: Teddi
authors.initials: T
authors.lastname: Hebert
authors.forename: Yuan-Di C
authors.initials: YD
authors.lastname: Halvorsen
authors.forename: Benjamin M
authors.initials: BM
authors.lastname: Buehrer
authors.forename: Jeffrey M
authors.initials: JM
authors.lastname: Gimble
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000378:metabolism; D050156:Adipogenesis / Q000235:genetics; D000273:Adipose Tissue / Q000166:cytology*; D001391:Azo Compounds / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods*; D006801:Humans; D009928:Organ Specificity / Q000235:genetics; D013194:Staining and Labeling; D013234:Stem Cells / Q000166:cytology*; D016707:Tissue Fixation; D015854:Up-Regulation / Q000235:genetics
chemical_list: D001391:Azo Compounds; C011049:oil red O