title: Quantum dot and Cy5.5 labeled nanoparticles to investigate lipoprotein biointeractions via Förster resonance energy transfer.
abstract: The study of lipoproteins, natural nanoparticles comprised of lipids and apolipoproteins that transport fats throughout the body, is of key importance to better understand, treat, and prevent cardiovascular disease. In the current study, we have developed a lipoprotein-based nanoparticle that consists of a quantum dot (QD) core and Cy5.5 labeled lipidic coating. The methodology allows judicious tuning of the QD/Cy5.5 ratio, which enabled us to optimize Förster resonance energy transfer (FRET) between the QD core and the Cy5.5-labeled coating. This phenomenon allowed us to study lipoprotein-lipoprotein interactions, lipid exchange dynamics, and the influence of apolipoproteins on these processes. Moreover, we were able to study HDL-cell interactions and exploit FRET to visualize HDL association with live macrophage cells.
authors.affiliation: Translational and Molecular Imaging Institute.
authors.forename: Torjus
authors.initials: T
authors.lastname: Skajaa
authors.forename: Yiming
authors.initials: Y
authors.lastname: Zhao
authors.forename: Dave J
authors.initials: DJ
authors.lastname: van den Heuvel
authors.forename: Hans C
authors.initials: HC
authors.lastname: Gerritsen
authors.forename: David P
authors.initials: DP
authors.lastname: Cormode
authors.forename: Rolf
authors.initials: R
authors.lastname: Koole
authors.forename: Matti M
authors.initials: MM
authors.lastname: van Schooneveld
authors.forename: Jan Andries
authors.initials: JA
authors.lastname: Post
authors.forename: Edward A
authors.initials: EA
authors.lastname: Fisher
authors.forename: Zahi A
authors.initials: ZA
authors.lastname: Fayad
authors.forename: Celso
authors.initials: C
authors.lastname: de Mello Donega
authors.forename: Andries
authors.initials: A
authors.lastname: Meijerink
authors.forename: Willem J M
authors.initials: WJ
authors.lastname: Mulder
mesh_terms: D031541:Fluorescence Resonance Energy Transfer; D008074:Lipoproteins / Q000378:metabolism*; D053758:Nanoparticles; D011485:Protein Binding; D045663:Quantum Dots
chemical_list: D008074:Lipoproteins