title: Enzymatic synthesis of sucrose octaacetate using a novel alkaline protease.
abstract: Acylation of 0.5 g sucrose with 1.2 ml acetic anhydride was carried out in 2 ml two-solvent medium of anhydrous pyridine/n-hexane (1:1, v/v) using 0.2 g crude protease from Serratia sp. Sucrose octaacetate was the sole product and more than 90% sucrose was converted in 24 h at 30°C. The purity of sucrose octaacetate reached 100%, via a simple purification method of alcohol/water washing and centrifugation.
authors.affiliation: Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Lihu Road 1800, Wuxi, 214122, Jiangsu, China.
authors.forename: Guiying
authors.initials: G
authors.lastname: Li
authors.forename: Yujie
authors.initials: Y
authors.lastname: Cai
authors.forename: Xiangru
authors.initials: X
authors.lastname: Liao
authors.forename: Jing
authors.initials: J
authors.lastname: Yin
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D002498:Centrifugation; D010450:Endopeptidases / Q000378:metabolism*; D006586:Hexanes / Q000737:chemistry; D011725:Pyridines / Q000737:chemistry; D012997:Solvents / Q000737:chemistry; D013395:Sucrose / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism*; D014867:Water / Q000737:chemistry
chemical_list: D001426:Bacterial Proteins; D006586:Hexanes; D011725:Pyridines; D012997:Solvents; D014867:Water; C026879:sucrose octaacetate; C026385:n-hexane; D013395:Sucrose; D010450:Endopeptidases; C087822:alkaline protease; C023666:pyridine