title: Low-dose irradiation causes rapid alterations to the proteome of the human endothelial cell line EA.hy926.
abstract: High doses of ionising radiation damage the heart by an as yet unknown mechanism. A concern for radiological protection is the recent epidemiological data indicating that doses as low as 100-500 mGy may induce cardiac damage. The aim of this study was to identify potential molecular targets and/or mechanisms involved in the pathogenesis of low-dose radiation-induced cardiovascular disease. The vascular endothelium plays a pivotal role in the regulation of cardiac function and is therefore a potential target tissue. We report here that low-dose radiation induced rapid and time-dependent changes in the cytoplasmic proteome of the human endothelial cell line EA.hy926. The proteomes were investigated at 4 and 24 h after irradiation at two different dose rates (Co-60 gamma ray total dose 200 mGy; 20 mGy/min and 190 mGy/min) using 2D-DIGE technology. Differentially expressed proteins were identified, after in-gel trypsin digestion, by MALDI-TOF/TOF tandem mass spectrometry, and peptide mass fingerprint analyses. We identified 15 significantly differentially expressed proteins, of which 10 were up-regulated and 5 down-regulated, with more than ±1.5-fold difference compared with unexposed cells. Pathways influenced by the low-dose exposures included the Ran and RhoA pathways, fatty acid metabolism and stress response.
authors.affiliation: Institute of Radiation Biology, Helmholtz Zentrum München, German Research Center for Environmental Health, Ingolstaedter Landstrasse 1, 85764, Neuherberg, Germany.
authors.forename: Franka
authors.initials: F
authors.lastname: Pluder
authors.forename: Zarko
authors.initials: Z
authors.lastname: Barjaktarovic
authors.forename: Omid
authors.initials: O
authors.lastname: Azimzadeh
authors.forename: Simone
authors.initials: S
authors.lastname: Mörtl
authors.forename: Anne
authors.initials: A
authors.lastname: Krämer
authors.forename: Sylvia
authors.initials: S
authors.lastname: Steininger
authors.forename: Hakan
authors.initials: H
authors.lastname: Sarioglu
authors.forename: Dariusz
authors.initials: D
authors.lastname: Leszczynski
authors.forename: Reetta
authors.initials: R
authors.lastname: Nylund
authors.forename: Arvi
authors.initials: A
authors.lastname: Hakanen
authors.forename: Arundhathi
authors.initials: A
authors.lastname: Sriharshan
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Atkinson
authors.forename: Soile
authors.initials: S
authors.lastname: Tapio
mesh_terms: D017209:Apoptosis / Q000528:radiation effects; D049109:Cell Proliferation / Q000528:radiation effects; D003600:Cytosol / Q000378:metabolism / Q000528:radiation effects; D004305:Dose-Response Relationship, Drug; D042783:Endothelial Cells / Q000166:cytology / Q000000981:diagnostic imaging* / Q000378:metabolism*; D020869:Gene Expression Profiling; D006801:Humans; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics; D011859:Radiography; D013997:Time Factors
chemical_list: D020543:Proteome