title: Phylogenetic analysis and evolution of aromatic amino acid hydroxylase.
abstract: A study was performed to investigate the phylogenetic relationship among AAAH members and to statistically evaluate sequence conservation and functional divergence. In total, 161 genes were identified from 103 species. Phylogenetic analysis showed that well-conserved subfamilies exist. Exon-intron structure analysis showed that the gene structures of AAAH were highly conserved across some different lineage species, while some species-specific introns were also found. The dynamic distribution of ACT domain suggested one gene fusion event has occurred in eukaryota. Significant functional divergence was found between some subgroups. Analysis of the site-specific profiles revealed critical amino acid residues for functional divergence. This study highlights the molecular evolution of this family and may provide a starting point for further experimental verifications.
authors.affiliation: Institute of Life Science, Jiangsu University, Zhenjiang, Jiangsu, PR China. cjinfor@163.com
authors.forename: Jun
authors.initials: J
authors.lastname: Cao
authors.forename: Feng
authors.initials: F
authors.lastname: Shi
authors.forename: Xiaoguang
authors.initials: X
authors.lastname: Liu
authors.forename: Guang
authors.initials: G
authors.lastname: Huang
authors.forename: Min
authors.initials: M
authors.lastname: Zhou
mesh_terms: D024322:Amino Acids, Aromatic / Q000378:metabolism*; D000818:Animals; D019295:Computational Biology / Q000379:methods*; D019143:Evolution, Molecular; D005091:Exons / Q000235:genetics; D006801:Humans; D007438:Introns / Q000235:genetics; D006899:Mixed Function Oxygenases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D010802:Phylogeny; D017434:Protein Structure, Tertiary; D012641:Selection, Genetic; D017386:Sequence Homology, Amino Acid
chemical_list: D024322:Amino Acids, Aromatic; D006899:Mixed Function Oxygenases