title: Hydrophoretic high-throughput selection of platelets in physiological shear-stress range.
abstract: A gentle, but fast means for low-stress, high-throughput platelet purification is of significant clinical and biotechnological utility. Current implementations to sort platelets, however, require an external physical field, specialized buffer, or the harsh separation condition of high shear stress that tends to cause platelet stimulation. Here we report the use of hydrophoretic size separation in a wider channel and its parallelization to augment its throughput capability, maintaining physiological shear-stress range. We demonstrate a parallelized device comprising 10 stacks of the wide-channel hydrophoresis device, yielding a throughput of 2.9 million cells s(-1) and a platelet purity of 76.8%. The use of the wide channel for hydrophoresis also facilitates clogging-free separation by sorting blood clots and plaques. The wide-channel hydrophoresis offers the potential for gentle, fast, clogging-free sorting of rare blood cells with extreme throughput capabilities.
authors.affiliation: Department of Bio and Brain Engineering, College of Life Science and Bioengineering, Korea Advanced Institute of Science and Technology, 335 Gwahangno, Yuseong-gu, Daejeon 305-701, Republic of Korea.
authors.forename: Sungyoung
authors.initials: S
authors.lastname: Choi
authors.forename: Taeyun
authors.initials: T
authors.lastname: Ku
authors.forename: Seungjeong
authors.initials: S
authors.lastname: Song
authors.forename: Chulhee
authors.initials: C
authors.lastname: Choi
authors.forename: Je-Kyun
authors.initials: JK
authors.lastname: Park
mesh_terms: D000818:Animals; D001709:Biotechnology; D001792:Blood Platelets / Q000737:chemistry*; D002469:Cell Separation; D055252:Cell-Derived Microparticles; D005434:Flow Cytometry; D057166:High-Throughput Screening Assays / Q000379:methods*; D015539:Platelet Activation; D051381:Rats; D017207:Rats, Sprague-Dawley; D033081:Shear Strength; D013314:Stress, Mechanical