title: Glucose-insulin infusion reduces kidney injury in an experimental model of ischemic nephropathy.
abstract: OBJECTIVE
  intensive insulin therapy may reduce renal dysfunction during severe illness in adult patients. We evaluated the effects of insulin-glucose (IG) in normoglycemic rats subjected to ischemia-reperfusion (I/R)-induced acute kidney injury.
  
  
  METHODS
  animals received intravenous infusions of 5% glucose [control (C)] or IG for 96 h. I/R was induced by means of bilateral renal artery clamping for 45 min. Serum creatinine (sCr) and urea (sUr) levels were evaluated before and up to 72 h after injury. Tissue samples were evaluated 72 h after I/R on a scale of 0 (normal) to 4 (above 75%) in relation to the extent of kidney injury.
  
  
  RESULTS
  after 48 h of I/R, sCr and sUr were increased 2- to 4-fold in C as compared to sham-operated controls (p < 0.05). IG produced significant improvements in renal function (p < 0.05). Upon histopathological analysis, the IG group presented less tubular damage in comparison to the C group: level 1, 60 versus 20%; level 2, 20 versus 30%; level 3, 20 versus 30%, and level 4, 0 versus 20%, respectively (n = 10; p = 0.057).
  
  
  CONCLUSIONS
  our results suggest that IG infusion attenuates the renal damage induced by severe I/R independently of blood glucose control. This strategy may constitute a therapeutic option for the prevention and treatment of ischemic renal injury.
authors.affiliation: Division of Nephrology, Medicine Department, Federal University of São Paulo, São Paulo, Brazil.
authors.forename: Rosilene S
authors.initials: RS
authors.lastname: Melo
authors.forename: Iria
authors.initials: I
authors.lastname: Visoná
authors.forename: Waldemar S
authors.initials: WS
authors.lastname: Almeida
authors.forename: Alexandre Holthausen
authors.initials: AH
authors.lastname: Campos
mesh_terms: D058186:Acute Kidney Injury / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology; D000818:Animals; D003404:Creatinine / Q000097:blood; D005947:Glucose / Q000008:administration & dosage / Q000627:therapeutic use*; D007328:Insulin / Q000008:administration & dosage / Q000627:therapeutic use*; D007668:Kidney / Q000473:pathology / Q000503:physiopathology; D008297:Male; D023421:Models, Animal; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000188:drug therapy*; D014508:Urea / Q000097:blood
chemical_list: D007328:Insulin; D014508:Urea; D003404:Creatinine; D005947:Glucose