title: Nucleoprotein-RNA orientation in the measles virus nucleocapsid by three-dimensional electron microscopy.
abstract: Recombinant measles virus nucleoprotein-RNA (N-RNA) helices were analyzed by negative-stain electron microscopy. Three-dimensional reconstructions of trypsin-digested and intact nucleocapsids coupled to the docking of the atomic structure of the respiratory syncytial virus (RSV) N-RNA subunit into the electron microscopy density map support a model that places the RNA at the exterior of the helix and the disordered C-terminal domain toward the helix interior, and they suggest the position of the six nucleotides with respect to the measles N protomer.
authors.affiliation: UVHCI, UMI 3265 UJF-EMBL-CNRS, BP 181, 38042 Grenoble, Cedex 9, France.
authors.forename: Ambroise
authors.initials: A
authors.lastname: Desfosses
authors.forename: Gaël
authors.initials: G
authors.lastname: Goret
authors.forename: Leandro
authors.initials: L
authors.lastname: Farias Estrozi
authors.forename: Rob W H
authors.initials: RW
authors.lastname: Ruigrok
authors.forename: Irina
authors.initials: I
authors.lastname: Gutsche
mesh_terms: D021621:Imaging, Three-Dimensional; D008459:Measles virus / Q000648:ultrastructure*; D008854:Microscopy, Electron / Q000379:methods; D008958:Models, Molecular; D019251:Nucleocapsid / Q000648:ultrastructure*; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins / Q000648:ultrastructure*; D012367:RNA, Viral / Q000648:ultrastructure*; D013194:Staining and Labeling / Q000379:methods; D014764:Viral Proteins / Q000648:ultrastructure*
chemical_list: D019590:Nucleocapsid Proteins; D009698:Nucleoproteins; D012367:RNA, Viral; D014764:Viral Proteins; C058314:nucleoprotein, Measles virus