title: Myocardial parvovirus B19 persistence: lack of association with clinicopathologic phenotype in adults with heart failure.
abstract: BACKGROUND
  Multiple viruses have been isolated from the heart, but their significance remains controversial. We sought to determine the prevalence of cardiotropic viruses in endomyocardial biopsy (EMB) samples from adult patients with heart failure (HF) and to define the clinicopathologic profile of patients exhibiting viral positivity.
  
  
  RESULTS
  EMB from 100 patients (median ejection fraction, 30%; interquartile range [IQR], 20% to 45%) presenting for cardiomyopathy evaluation (median symptom duration, 5 months; IQR, 1 to 13 months) were analyzed by polymerase chain reaction for adenovirus, cytomegalovirus, enteroviruses, Epstein-Barr virus, and parvovirus B19. Each isolate was sequenced, and viral load was determined. Parvovirus B19 was the only virus detected in EMB samples (12% of subjects). No patient had antiparvovirus IgM antibodies, but all had IgG antibodies, suggesting viral persistence. The clinical presentation of parvovirus-positive patients was markedly heterogeneous with both acute and chronic HF, variable ventricular function, and ischemic cardiomyopathy. No patient met Dallas histopathologic criteria for active or borderline myocarditis. Two patients with a positive cardiac MRI and presumed "parvomyocarditis" had similar viral loads to autopsy controls without heart disease. The oldest parvovirus-positive patients were positive for genotype 2, suggesting lifelong persistence in the myocardium.
  
  
  CONCLUSIONS
  Parvovirus B19 was the only virus isolated from EMB samples in this series of adult patients with HF from the United States. Positivity was associated with a wide array of clinical presentations and HF phenotypes. Our studies do not support a causative role for parvovirus B19 persistence in HF and, therefore, advocate against the use of antiviral therapy for these patients.
authors.affiliation: Division of Cardiovascular Medicine, Brigham and Women's Hospital, Boston, MA, USA.
authors.forename: Garrick C
authors.initials: GC
authors.lastname: Stewart
authors.forename: Javier
authors.initials: J
authors.lastname: Lopez-Molina
authors.forename: Raju V S R K
authors.initials: RV
authors.lastname: Gottumukkala
authors.forename: Gregg F
authors.initials: GF
authors.lastname: Rosner
authors.forename: Mary S
authors.initials: MS
authors.lastname: Anello
authors.forename: Jonathan L
authors.initials: JL
authors.lastname: Hecht
authors.forename: Gayle L
authors.initials: GL
authors.lastname: Winters
authors.forename: Robert F
authors.initials: RF
authors.lastname: Padera
authors.forename: Kenneth L
authors.initials: KL
authors.lastname: Baughman
authors.forename: Myra A
authors.initials: MA
authors.lastname: Lipes
mesh_terms: D000328:Adult; D000368:Aged; D001706:Biopsy; D004279:DNA, Viral / Q000097:blood; D018450:Disease Progression; D005260:Female; D006321:Heart / Q000821:virology*; D006333:Heart Failure / Q000097:blood / Q000473:pathology* / Q000821:virology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009206:Myocardium / Q000473:pathology*; D010322:Parvoviridae Infections / Q000453:epidemiology; D016732:Parvovirus B19, Human / Q000235:genetics / Q000302:isolation & purification*; D010641:Phenotype; D015995:Prevalence; D012189:Retrospective Studies; D019562:Viral Load
chemical_list: D004279:DNA, Viral