title: Real-time TaqMan RT-PCR for detection of maize chlorotic mottle virus in maize seeds.
abstract: Maize chlorotic mottle virus (MCMV) causes corn lethal necrosis disease, and can be transmitted through infected maize seeds. It remains a challenge to detect this virus in the seeds to prevent its introduction and infection. For this purpose, a real-time TaqMan RT-PCR procedure for efficient detection of MCMV was developed. The sensitivity of the method was 4 fg of total RNA or 25 copies of RNA transcripts, which was approximately ten-fold higher than conventional RT-PCR gel electrophoresis method. The successful detection of MCMV in maize seeds suggested the feasibility of this procedure for routine testing.
authors.affiliation: State Key Laboratory of Agro-biotechnology and Department of Plant Pathology, China Agricultural University, Haidian District, Beijing, China.
authors.forename: Yongjiang
authors.initials: Y
authors.lastname: Zhang
authors.forename: Wenjun
authors.initials: W
authors.lastname: Zhao
authors.forename: Mingfu
authors.initials: M
authors.lastname: Li
authors.forename: Hongjun
authors.initials: H
authors.lastname: Chen
authors.forename: Shuifang
authors.initials: S
authors.lastname: Zhu
authors.forename: Zaifeng
authors.initials: Z
authors.lastname: Fan
mesh_terms: D017931:DNA Primers / Q000235:genetics; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000821:virology*; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012639:Seeds / Q000821:virology*; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D019183:Tombusviridae / Q000302:isolation & purification*; D003313:Zea mays / Q000821:virology*
chemical_list: D017931:DNA Primers; D012367:RNA, Viral