title: The role of sarcoplasmic protein in hydrostatic pressure-induced myofibrillar protein denaturation.
abstract: To observe the role of sarcoplasmic protein (SP) on myofibrillar protein (MP) denaturation under a hydrostatic pressure (HP), MP isolated from bovine muscle was treated with 300 MPa by increasing concentrations of SP (0, 0.8, 1.6, and 3.2 mg/ml) from bovine. SDS-PAGE patterns of soluble proteins in 0.1M NaCl (pH 7.4) indicated that a protein (about 100 kDa) from MP decreased with increasing concentrations of SP and that a 97 kDa protein from SP observed with 0.1 MPa was not observed with 300 MPa. SDS-PAGE patterns of soluble proteins in 0.6 M NaCl (pH 7.4) and Ca-ATPase activity showed that the denaturation of myosin heavy chain (MHC) was accelerated with increasing SP concentrations with the 300 MPa treatment. Thus, the addition of SP enhanced HP-induced denaturation of MHC and of a protein from MP of about 100 kDa.
authors.affiliation: Division for Food Industry Platform Technology, Korea Food Research Institute, Seongnam-si, Gyeonggi-do 463-746, Republic of Korea.
authors.forename: Eun-Jung
authors.initials: EJ
authors.lastname: Lee
authors.forename: Young-Ho
authors.initials: YH
authors.lastname: Kim
authors.forename: Nam-Hyouck
authors.initials: NH
authors.lastname: Lee
authors.forename: Suk-In
authors.initials: SI
authors.lastname: Hong
authors.forename: Katsuhiro
authors.initials: K
authors.lastname: Yamamoto
authors.forename: Yun-Ji
authors.initials: YJ
authors.lastname: Kim
mesh_terms: D000185:Actinin / Q000737:chemistry; D000818:Animals; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel; D005511:Food Handling; D005782:Gels; D024982:Glycogen Phosphorylase, Muscle Form; D006874:Hydrostatic Pressure / Q000009:adverse effects; D008461:Meat Products / Q000032:analysis; D008970:Molecular Weight; D009124:Muscle Proteins / Q000032:analysis / Q000737:chemistry*; D009210:Myofibrils / Q000737:chemistry*; D018995:Myosin Heavy Chains / Q000737:chemistry; D011489:Protein Denaturation; D012519:Sarcoplasmic Reticulum / Q000737:chemistry*; D012995:Solubility
chemical_list: D005782:Gels; D009124:Muscle Proteins; D000185:Actinin; D024982:Glycogen Phosphorylase, Muscle Form; D017301:Ca(2+) Mg(2+)-ATPase; D018995:Myosin Heavy Chains; D000252:Calcium-Transporting ATPases