title: Nanofibers from blends of polyvinyl alcohol and polyhydroxy butyrate as potential scaffold material for tissue engineering of skin.
abstract: Nanofibers were prepared by electrospinning from pure polyvinyl alcohol (PVA), polyhydroxybutyrate (PHB), and their blends. Miscibility and morphology of both polymers in the nanofiber blends were studied by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and differential scanning calorimetry (DSC), revealing that PVA and PHB were miscible with good compatibility. DSC also revealed suppression of crystallinity of PHB in the blend nanofibers with increasing proportion of PVA. The hydrolytic degradation of PHB was accelerated with increasing PVA fraction. Cell culture experiments with a human keratinocyte cell line (HaCaT) and dermal fibroblast on the electrospun PHB and PVA/PHB blend nanofibers showed maximum adhesion and proliferation on pure PHB. However, the addition of 5 wt % PVA to PHB inhibited growth of HaCaT cells but not of fibroblasts. On the contrary, adhesion and proliferation of HaCaT cells were promoted on PVA/PHB (50/50) fibers, which inhibited growth of fibroblasts.
authors.affiliation: Institute of Physics, Martin Luther University Halle-Wittenberg, von Danckelmann Platz 3, D-06099 Halle/S., Germany. ashraf.abdel-sayed@physik.uni-halle.de
authors.forename: Ashraf Sh
authors.initials: ASh
authors.lastname: Asran
authors.forename: Khashayar
authors.initials: K
authors.lastname: Razghandi
authors.forename: Neha
authors.initials: N
authors.lastname: Aggarwal
authors.forename: Goerg H
authors.initials: GH
authors.lastname: Michler
authors.forename: T
authors.initials: T
authors.lastname: Groth
mesh_terms: D002448:Cell Adhesion; D002460:Cell Line; D049109:Cell Proliferation; D002623:Chemistry Techniques, Analytical; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D006801:Humans; D006885:Hydroxybutyrates / Q000737:chemistry*; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects; D057139:Nanofibers / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry*; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D000091402:Prohibitins; D012867:Skin / Q000166:cytology / Q000187:drug effects*; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds; D000094122:Polyhydroxybutyrates
chemical_list: D006885:Hydroxybutyrates; D011091:Polyesters; D011142:Polyvinyl Alcohol; D000091402:Prohibitins; C000717328:PHB protein, human; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates