title: [Transthyretin-binding activity of hexabromocyclododecanes (HBCDs) and its thyroid hormone disrupting effects after developmental exposure].
abstract: In vivo and in vitro research approaches were carried out to survey the potential health risk of environmental exposure by hexabromocyclododecanes (HBCDs). Transthyretin-binding assay was designed to test for the potency of HBCDs to compete with thyroxine (T4) for binding to the transport protein. The results showed that the binding of 25I-T4 and T4 was only slightly inhabited even at the highest competitive concentration of HBCDs (75.08%, 80 micromol x L(-1)), indicating the marginally interfere potency of HBCDs in the transportation of T4. Sprague-Dawley rats of 3-days old were exposed to 0.2 mg/kg and 1 mg/kg HBCDs for 21 d to examine the thyroid hormones (THs) disrupting effects of HBCDs after developmental exposure. Compared with the controls, levels of total 3,3',5-triiodothyronine (TT3), free 3,3',5-triiodothyronine (FT3), increased significantly (p < 0.05, p < 0.05) in low- and high-dose exposures, thyroid stimulating hormone (TSH) also increased slightly while the total thyroxine (TT4), free thyroxine (FT4) had a decline about two-fold inversely. Combined both the in vivo and in vitro results, the possible mode of action of HBCDs on THs disruption may through the synergy or substitution effect of T3. The findings support further investigation of the potential THs disrupting effects of HBCDs on public health, especially on children during brain development.
authors.affiliation: Department of Environmental Science, Donghua University, Shanghai 201620, China. ling_jx@dhu.edu.cn
authors.forename: Xiu-Ling
authors.initials: XL
authors.lastname: Ji
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
authors.forename: Yue
authors.initials: Y
authors.lastname: Lu
authors.forename: Gao-Ren
authors.initials: GR
authors.lastname: Zhong
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001667:Binding, Competitive / Q000187:drug effects; D052244:Endocrine Disruptors / Q000378:metabolism / Q000633:toxicity*; D004781:Environmental Exposure; D004785:Environmental Pollutants / Q000378:metabolism / Q000633:toxicity*; D006842:Hydrocarbons, Brominated / Q000378:metabolism / Q000633:toxicity*; D011228:Prealbumin / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013963:Thyroid Hormones / Q000378:metabolism*
chemical_list: D052244:Endocrine Disruptors; D004785:Environmental Pollutants; D006842:Hydrocarbons, Brominated; D011228:Prealbumin; D013963:Thyroid Hormones; C089796:hexabromocyclododecane