title: Myocardium functional recovery protection by omeprazole after ischemia-reperfusion in isolated rat hearts.
abstract: OBJECTIVE
  To evaluate the myocardium contractility alterations of isolated hearts of rats, submitted to ischemia and reperfusion with and without administration of the omeprazole.
  
  
  METHODS
  Twelve Wistar breed rats with 270 g mean body weight was studied. After anesthesia by intraperitoneal injection of ketamine 10mg and xylazine 2mg, their hearts were removed and perfused with Krebs-Henseleit solution (95% of O2 and 5% of CO2, 37 ºC, 110-120 mmHg perfusion pressure, 8 mmHg ventricular diastolic pressure) in the São Francisco de Assis disposable Langendorff system model Comex Ltda, MG. The six hearts of Group I (GI) and of the Group II (GII) were submitted to 20 min ischemia and 30 min reperfusion. In GII hearts, intracoronary injection of omeprazole 200 mcg was done immediately before the ischemia period induction. The following parameters were registered after the stabilization period (t0), and after the reperfusion period (t30): heart rate (HR), coronary flow (CoF), systolic pressure (SP), +dP/dt and -dP/dt. The Kruskal-Wallis test (P<0.05) was applied to statistical analysis.
  
  
  RESULTS
  There were no significant differences (P>0.05) between groups among HR and CoF values. Differences (P<0.05) occurred between groups, I e II after the reperfusion period (t30) regarding systolic pressure reduced for 28.0 ± 3.6% in the control group GI and for 79.0 ± 5.9% in GII; The +dP/dtmax declined to be only 31.0 ± 5.6% in GI, preserving 99.4 ± 11.2% values in GII (P<0.05). The t30 -dP/dtmax values were GI 26.0 ± 7.3% and GII 82.0 ± 2.2% (P<0.05).
  
  
  CONCLUSIONS
  The omeprazole administration before ischemia induction significantly protected the myocardium function recovery.
authors.affiliation: Departamento de Cirurgia, São Francisco de Assis Cardiovascular Foundation-Verdade é Jesus, Belo Horizonte, MG, Brazil. gomes@servcor.com
authors.forename: Otoni Moreira
authors.initials: OM
authors.lastname: Gomes
authors.forename: Mônica de Mônico
authors.initials: Mde M
authors.lastname: Magalhães
authors.forename: Rafael Diniz
authors.initials: RD
authors.lastname: Abrantes
mesh_terms: D000818:Animals; D004791:Enzyme Inhibitors / Q000494:pharmacology / Q000627:therapeutic use*; D006339:Heart Rate / Q000187:drug effects; D009200:Myocardial Contraction / Q000187:drug effects*; D015428:Myocardial Reperfusion Injury / Q000188:drug therapy*; D009853:Omeprazole / Q000494:pharmacology / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D020127:Recovery of Function / Q000187:drug effects
chemical_list: D004791:Enzyme Inhibitors; D009853:Omeprazole