title: Comprehensive & cost effective laboratory monitoring of HIV/AIDS: an African role model.
abstract: We present the video about assisting anti-retroviral therapy (ART) by an apt laboratory service - representing a South-African role model for economical large scale diagnostic testing. In the low-income countries inexpensive ART has transformed the prospects for the survival of HIV seropositive patients but there are doubts whether there is a need for the laboratory monitoring of ART and at what costs - in situations when the overall quality of pathology services can still be very low. The appropriate answer is to establish economically sound services with better coordination and stricter internal quality assessment than seen in western countries. This video, photographed at location in the National Health Laboratory Services (NHLS-SA) at the Witwatersrand University, Johannesburg, South Africa, provides such a coordinated scheme expanding the original 2-color CD4-CD45 PanLeucoGating strategy (PLG). Thus the six modules of the video presentation reveal the simplicity of a 4-color flow cytometric assay to combine haematological, immunological and virology-related tests in a single tube. These video modules are: (i) the set-up of instruments; (ii) sample preparations; (iii) testing absolute counts and monitoring quality for each sample by bead-count-rate; (iv) the heamatological CD45 test for white cell counts and differentials; (v) the CD4 counts, and (vi) the activation of CD8+ T cells measured by CD38 display, a viral load related parameter. The potential cost-savings are remarkable. This arrangement is a prime example for the feasibility of performing > 800-1000 tests per day with a stricter quality control than that applied in western laboratories, and also with a transfer of technology to other laboratories within a NHLS-SA network. Expert advisors, laboratory managers and policy makers who carry the duty of making decisions about introducing modern medical technology are frequently not in a position to see the latest technical details as carried out in the large regional laboratories with huge burdens of workload. Hence this video shows details of these new developments.
authors.affiliation: National Health Laboratory Services.
authors.forename: Denise
authors.initials: D
authors.lastname: Lawrie
authors.forename: George
authors.initials: G
authors.lastname: Janossy
authors.forename: Maarten
authors.initials: M
authors.lastname: Roos
authors.forename: Deborah K
authors.initials: DK
authors.lastname: Glencross
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000188:drug therapy / Q000191:economics / Q000276:immunology*; D044966:Anti-Retroviral Agents / Q000627:therapeutic use; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D003362:Cost-Benefit Analysis; D005434:Flow Cytometry / Q000191:economics / Q000379:methods*; D015658:HIV Infections / Q000188:drug therapy / Q000191:economics / Q000276:immunology*; D006801:Humans; D018655:Lymphocyte Count / Q000191:economics / Q000379:methods; D013019:South Africa; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology
chemical_list: D044966:Anti-Retroviral Agents