title: Effect of a scaffold fabricated thermally from acetylated PLGA on the formation of engineered cartilage.
abstract: A MHDS has been employed to fabricate 3D scaffolds from PLGA with acetyl endgroups to achieve in vivo regeneration of cartilage tissue. The fabricated acetylated-PLGA scaffold showed open pores and interconnected structures. Rabbit chondrocytes were seeded on the PLGA scaffolds and transplanted immediately into subcutaneous sites of athymic mice. Chondrocytes transplantation with untreated PLGA scaffolds served as a control. Histological analysis of the implants at 4 weeks with H&E staining and alcian blue staining revealed higher extracellular matrix and GAG expression at the neocartilage in the PLGA-6Ac scaffolds than that of the PLGA-6OH scaffold group. This endgroup-modified scaffold may be useful for successful cartilage tissue engineering in orthopedic applications.
authors.affiliation: Department of Biomedical Science, CHA University, Seoul 135-081, Korea.
authors.forename: Sun-Woong
authors.initials: SW
authors.lastname: Kang
authors.forename: Suk-Jun
authors.initials: SJ
authors.lastname: Lee
authors.forename: Jin-Su
authors.initials: JS
authors.lastname: Kim
authors.forename: Eun-Hee
authors.initials: EH
authors.lastname: Choi
authors.forename: Byung-Hyun
authors.initials: BH
authors.lastname: Cha
authors.forename: Jin-Hyung
authors.initials: JH
authors.lastname: Shim
authors.forename: Dong-Woo
authors.initials: DW
authors.lastname: Cho
authors.forename: Soo-Hong
authors.initials: SH
authors.lastname: Lee
mesh_terms: D000107:Acetylation / Q000187:drug effects; D000818:Animals; D002356:Cartilage / Q000166:cytology / Q000187:drug effects*; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology / Q000187:drug effects; D020219:Chondrogenesis / Q000187:drug effects / Q000235:genetics; D003470:Culture Media / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects; D019344:Lactic Acid / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D011100:Polyglycolic Acid / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011817:Rabbits; D013696:Temperature; D013818:Thermogravimetry; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D003470:Culture Media; D012333:RNA, Messenger; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid