title: Development of a high density integrated reference genetic linkage map for the multinational Brassica rapa Genome Sequencing Project.
abstract: We constructed a high-density Brassica rapa integrated linkage map by combining a reference genetic map of 78 doubled haploid lines derived from Chiifu-401-42 × Kenshin (CKDH) and a new map of 190 F2 lines derived from Chiifu-401-42 × rapid cycling B. rapa (CRF2). The integrated map contains 1017 markers and covers 1262.0 cM of the B. rapa genome, with an average interlocus distance of 1.24 cM. High similarity of marker order and position was observed among the linkage groups of the maps with few short-distance inversions. In total, 155 simple sequence repeat (SSR) markers, anchored to 102 new bacterial artificial chromosomes (BACs) and 146 intron polymorphic (IP) markers were mapped in the integrated map, which would be helpful to align the sequenced BACs in the ongoing multinational Brassica rapa Genome Sequencing Project (BrGSP). Further, comparison of the B. rapa consensus map with the 10 B. juncea A-genome linkage groups by using 98 common IP markers showed high-degree colinearity between the A-genome linkage groups, except for few markers showing inversion or translocation. Suggesting that chromosomes are highly conserved between these Brassica species, although they evolved independently after divergence. The sequence information coming out of BrGSP would be useful for B. juncea breeding. and the identified Arabidopsis chromosomal blocks and known quantitative trait loci (QTL) information of B. juncea could be applied to improve other Brassica crops including B. rapa.
authors.affiliation: Molecular Genetics and Genomics Lab, Department of Horticulture, Chungnam National University, Gung-Dong, Yuseong-Gu, Daejeon 305 764, Republic of Korea.
authors.forename: Xiaonan
authors.initials: X
authors.lastname: Li
authors.forename: Nirala
authors.initials: N
authors.lastname: Ramchiary
authors.forename: Su Ryun
authors.initials: SR
authors.lastname: Choi
authors.forename: Dan
authors.initials: D
authors.lastname: Van Nguyen
authors.forename: Md Jamil
authors.initials: MJ
authors.lastname: Hossain
authors.forename: Hyeon Kook
authors.initials: HK
authors.lastname: Yang
authors.forename: Yong Pyo
authors.initials: YP
authors.lastname: Lim
mesh_terms: D017360:Arabidopsis / Q000235:genetics; D029741:Brassica rapa / Q000235:genetics*; D002874:Chromosome Mapping; D022202:Chromosomes, Artificial, Bacterial; D018745:Genome, Plant; D018895:Microsatellite Repeats; D040641:Quantitative Trait Loci; D017422:Sequence Analysis, DNA