title: Cytotoxicity of δ-tocotrienols from Kielmeyera coriacea against cancer cell lines.
abstract: In the search for new anti-cancer compounds, Brazilian Cerrado plant species have been investigated. The hexane root bark extract of Kielmeyera coriacea lead to a mixture of δ-tocotrienol (1) and its dimer (2). The structures of both compounds 1 and 2 were established based on detailed 1D and 2D NMR and EI-MS analyses. The cytotoxicity of the mixture was tested against four human tumor cell lines in the following cultures: MDA-MB-435 (melanoma), HCT-8 (colon), HL-60 (leukemia), and SF-295 (glioblastoma), and displayed IC(50) values ranging from 8.08 to 23.58μg/mL. Additional assays were performed in order to investigate the mechanism of action of the mixture (1+2) against the human leukemia cell line HL-60. The results suggested that the mixture suppressed leukemia growth and reduced cell survival, triggering both apoptosis and necrosis, depending on the concentration.
authors.affiliation: Laboratório de Farmacognosia, Universidade de Brasília, Brasília, Brazil.
authors.forename: Mariana Laundry
authors.initials: ML
authors.lastname: de Mesquita
authors.forename: Renata Mendonça
authors.initials: RM
authors.lastname: Araújo
authors.forename: Daniel Pereira
authors.initials: DP
authors.lastname: Bezerra
authors.forename: Raimundo Braz
authors.initials: RB
authors.lastname: Filho
authors.forename: José Elias
authors.initials: JE
authors.lastname: de Paula
authors.forename: Edilberto Rocha
authors.initials: ER
authors.lastname: Silveira
authors.forename: Cláudia
authors.initials: C
authors.lastname: Pessoa
authors.forename: Manoel Odorico
authors.initials: MO
authors.lastname: de Moraes
authors.forename: Letícia Veras
authors.initials: LV
authors.lastname: Costa Lotufo
authors.forename: Laila Salmen
authors.initials: LS
authors.lastname: Espindola
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004261:DNA Replication / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D019684:Magnoliopsida / Q000737:chemistry*; D008856:Microscopy, Fluorescence; D021241:Spectrometry, Mass, Electrospray Ionization; D024508:Tocotrienols / Q000494:pharmacology*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D024508:Tocotrienols