title: Pancreatitis-associated protein-I and pancreatitis-associated protein-III expression in a rat model of kainic acid-induced seizure.
abstract: The pancreatitis-associated protein (PAP) family (also known as the regenerating gene (Reg) family) is a group of 16 kDa secretory proteins structurally classified as the calcium dependent-type lectin superfamily. Some PAP family members are expressed in neurons following peripheral nerve injury and traumatic brain injury. To determine whether PAP family members are expressed in non-traumatic brain injury, expressions were analyzed following kainic acid (KA)-induced seizure. PAP-I (also known as Reg2 in rat and RegIII-beta in mouse) and pancreatitis associated protein-III (PAP-III; RegIII-gamma in mouse) messenger ribonucleic acid (mRNA) was transiently expressed in some restricted areas, such as the hippocampus and parahippocampal area; expression was observed immediately at a maximal level 1 day after seizure. Expression disappeared within 3 days after seizure. In situ hybridization (ISH) and immunohistochemistry revealed neuronal PAP-I and PAP-III expression in the hippocampal dentate gyrus, perirhinal and entorhinal cortices, and the posterior cortical nucleus of the amygdala. The number of PAP-III mRNA-positive neurons was significantly greater than PAP-I mRNA-positive neurons. The majority of positive neurons co-localized with c-Jun, but not with glutamic acid decarboxylase (GAD). These results may suggest that PAP-I and PAP-III induction in non-GABAergic neurons would protect neurons against damage following seizure.
authors.affiliation: Department of Anatomy and Neurobiology, Osaka City University, Graduate School of Medicine, Osaka 545-8585, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Kawahara
authors.forename: H
authors.initials: H
authors.lastname: Konishi
authors.forename: M
authors.initials: M
authors.lastname: Morino
authors.forename: K
authors.initials: K
authors.lastname: Ohata
authors.forename: H
authors.initials: H
authors.lastname: Kiyama
mesh_terms: D000626:Aminopeptidases / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology; D000818:Animals; D000951:Antigens, Neoplasm / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology; D014408:Biomarkers, Tumor / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology; D004195:Disease Models, Animal; D004833:Epilepsy, Temporal Lobe / Q000378:metabolism* / Q000517:prevention & control; D007608:Kainic Acid / Q000037:antagonists & inhibitors / Q000633:toxicity; D037181:Lectins, C-Type / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology; D008297:Male; D009498:Neurotoxins / Q000037:antagonists & inhibitors / Q000633:toxicity; D000073718:Pancreatitis-Associated Proteins; D051381:Rats; D017208:Rats, Wistar; D012640:Seizures / Q000139:chemically induced / Q000378:metabolism* / Q000517:prevention & control; D013702:Temporal Lobe / Q000378:metabolism* / Q000503:physiopathology; D005680:gamma-Aminobutyric Acid / Q000502:physiology
chemical_list: D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D037181:Lectins, C-Type; D009498:Neurotoxins; D000073718:Pancreatitis-Associated Proteins; C000612141:REG3A protein, human; C000612142:Reg2 protein, rat; C000612144:Reg3b protein, rat; C000612145:Reg3g protein, rat; D005680:gamma-Aminobutyric Acid; D000626:Aminopeptidases; C497676:pyroglutamyl aminopeptidase I, rat; D007608:Kainic Acid