title: In vitro growth of microsporidia Anncaliia algerae in cell lines from warm water fish.
abstract: Anncaliia algerae is an aquatic microsporidium that most commonly infects mosquitoes but can be grown on the rabbit kidney cell line, RK-13. Spores were purified from RK-13 cultures and added to cell lines from warm water fish and from an insect. The cell lines were GFSK-S1 and GFB3C-W1 from goldfish skin and brain respectively, ZEB2J from zebrafish embryos, FHMT-W1 from fathead minnow testis, and Sf9 from ovaries of a fall armyworm moth. All cultures were maintained at 27°C. Infection was judged to have taken place by the appearance of sporonts and/or spores in cells and occurred in all cell lines. Spores were also isolated from ZEB2J cultures and used to successfully infect new cultures of ZEB2J, RK-13 and Sf9. These results suggest that cells of a wide range of vertebrates support A. algerae growth in vitro and fish cells can produce spores infectious to cells of mammals, fish, and insects.
authors.affiliation: Department of Biology, University of Waterloo, 200 University Avenue West, Waterloo, ON, Canada. rmonagha@uwaterloo.ca
authors.forename: S Richelle
authors.initials: SR
authors.lastname: Monaghan
authors.forename: Rebecca L
authors.initials: RL
authors.lastname: Rumney
authors.forename: Nguyen T K
authors.initials: NT
authors.lastname: Vo
authors.forename: Niels C
authors.initials: NC
authors.lastname: Bols
authors.forename: Lucy E J
authors.initials: LE
authors.lastname: Lee
mesh_terms: D000818:Animals; D016817:Apansporoblastina / Q000166:cytology / Q000254:growth & development* / Q000472:pathogenicity; D018929:Cell Culture Techniques; D002460:Cell Line; D005399:Fishes / Q000382:microbiology*; D006054:Goldfish / Q000382:microbiology; D011817:Rabbits; D013170:Spores / Q000166:cytology / Q000254:growth & development; D015027:Zebrafish / Q000382:microbiology