title: Tape transfer sectioning of tissue microarrays introduces nonspecific immunohistochemical staining artifacts.
abstract: Tissue microarrays place tens to hundreds of formalin fixed, paraffin embedded tissue cores into a paraffin block in a systematic grid pattern that permits their simultaneous evaluation in a single section. The fragmented nature of the tissue cores often makes sectioning of tissue microarrays difficult so that the resulting disks of tissue lose their shape, fracture or fall out of the paraffin section altogether. We have evaluated an alternative sectioning protocol for stabilizing the tissue microarray surface by placing an adhesive tape "window" over the face of the paraffin block prior to sectioning. Once sectioned, the tape/sections are transferred directly onto coated microscope slides, thereby avoiding routine floating of sections on a water bath. After sectioning with either the tape transfer or standard protocols, slides were stained either using hematoxylin and eosin or immunohistochemistry using antibodies to S-100 protein and the tissue specific antigens, keratin (AE1/3) and the leukocyte common antigen CD45. We found that the tape method produced thicker sections that were darker and more densely packed with loss of tissue definition compared to sections prepared using water bath flotation. Quantitative image analysis of immunohistochemical staining demonstrated that the tape method produced a higher incidence of nonspecific staining, which raised the potential for false positive staining.
authors.affiliation: Biospecimens Research Group, and Tumour Bank The Children's Cancer Research Unit, The Children's Hospital at Westmead, Westmead, NSW, Australia. danielc@chw.edu.au
authors.forename: D
authors.initials: D
authors.lastname: Catchpoole
authors.forename: N
authors.initials: N
authors.lastname: Mackie
authors.forename: S
authors.initials: S
authors.lastname: McIver
authors.forename: A
authors.initials: A
authors.lastname: Chetcuti
authors.forename: A
authors.initials: A
authors.lastname: Henwood
authors.forename: N
authors.initials: N
authors.lastname: Graf
authors.forename: S
authors.initials: S
authors.lastname: Arbuckle
mesh_terms: D016477:Artifacts; D016591:Histocytological Preparation Techniques; D007150:Immunohistochemistry; D008867:Microtomy; D016612:Paraffin Embedding / Q000379:methods; D011786:Quality Control; D058996:Quality Improvement; D013048:Specimen Handling / Q000295:instrumentation* / Q000379:methods; D013194:Staining and Labeling; D046888:Tissue Array Analysis / Q000295:instrumentation* / Q000379:methods; D016610:Tissue Embedding / Q000379:methods