title: Chlorhexidine controlled-release profile after EDTA root surface etching: an in vivo study.
abstract: BACKGROUND
  The main objective of the present study was to quantify chlorhexidine (CHX) release after the use of CHX-EDTA root surface treatment as a local-delivery antimicrobial vehicle.
  
  
  METHODS
  Twenty non-smoking patients clinically diagnosed as having moderate-to-severe chronic periodontitis were selected to participate in this study. After cause-related therapy, one site in every patient received defect overfill with CHX gel 2% (20 sites). In addition, twenty contralateral sites received defect fill of CHX gel after 3 minutes of 24% EDTA gel root surface etching (20 sites). Gingival crevicular fluid samples were collected at 1, 3, 7, and 14 days post-therapy.
  
  
  RESULTS
  The CHX-EDTA group showed statistically significantly higher levels of CHX than those of the control group at 1, 3, and 7 days. At 14 days, the CHX-EDTA group showed 0.8 mg/mL values.
  
  
  CONCLUSIONS
  The use of CHX-EDTA root surface treatment as a local-delivery antimicrobial improves CHX substantivity.
authors.affiliation: Department of Periodontology, Faculty of Dental Medicine, Al-Azhar University, Cairo, Egypt. hgamal1@hotmail.com
authors.forename: Ahmed Y
authors.initials: AY
authors.lastname: Gamal
authors.forename: Radi M
authors.initials: RM
authors.lastname: Kumper
authors.forename: Hesham S
authors.initials: HS
authors.lastname: Sadek
authors.forename: Mahmoud T
authors.initials: MT
authors.lastname: El Destawy
mesh_terms: D000134:Acid Etching, Dental / Q000379:methods*; D000328:Adult; D016301:Alveolar Bone Loss / Q000188:drug therapy / Q000628:therapy; D000891:Anti-Infective Agents, Local / Q000008:administration & dosage*; D002614:Chelating Agents / Q000627:therapeutic use*; D002710:Chlorhexidine / Q000008:administration & dosage*; D002851:Chromatography, High Pressure Liquid; D055113:Chronic Periodontitis / Q000188:drug therapy* / Q000628:therapy; D003692:Delayed-Action Preparations; D003774:Dental Plaque Index; D012534:Dental Scaling; D004492:Edetic Acid / Q000627:therapeutic use*; D005260:Female; D005500:Follow-Up Studies; D005883:Gingival Crevicular Fluid / Q000737:chemistry; D006801:Humans; D008297:Male; D008875:Middle Aged; D009910:Oral Hygiene; D017622:Periodontal Attachment Loss / Q000188:drug therapy / Q000628:therapy; D010512:Periodontal Index; D010514:Periodontal Pocket / Q000188:drug therapy / Q000628:therapy; D016745:Root Planing; D013357:Subgingival Curettage; D014092:Tooth Root / Q000187:drug effects*
chemical_list: D000891:Anti-Infective Agents, Local; D002614:Chelating Agents; D003692:Delayed-Action Preparations; D004492:Edetic Acid; D002710:Chlorhexidine