title: Berberine attenuates lipopolysaccharide-induced impairments of intestinal glutamine transport and glutaminase activity in rat.
abstract: Berberine was reported to protect against the intestinal injury and improve the survival rate in sepsis, and glutamine deficiency was considered to be correlated with mortality in sepsis. We found that berberine pretreatment ameliorated lipopolysaccharide-induced direct intestinal injury and mucosal hypoplasia and attenuated impairments of intestinal glutamine transport and glutaminase activity, B(0)AT1 mRNA and protein expressions, and glutaminase protein expression. These findings showed the first time that berberine pretreatment could improve intestinal recovery and attenuate the impairment of glutamine transport and glutaminase activity in rat sepsis. This might be one of the mechanisms for the beneficial effect of berberine on sepsis.
authors.affiliation: School of Medicine, Najing University, Nanjing, Jiangsu, PR China.
authors.forename: Lingying
authors.initials: L
authors.lastname: Niu
authors.forename: Wei
authors.initials: W
authors.lastname: Qiao
authors.forename: Zhendong
authors.initials: Z
authors.lastname: Hu
authors.forename: Ning
authors.initials: N
authors.lastname: Li
authors.forename: Qian
authors.initials: Q
authors.lastname: Huang
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Gong
authors.forename: Qiurong
authors.initials: Q
authors.lastname: Li
authors.forename: Weiming
authors.initials: W
authors.lastname: Zhu
authors.forename: Jieshou
authors.initials: J
authors.lastname: Li
mesh_terms: D026921:Amino Acid Transport Systems, Neutral / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001599:Berberine / Q000494:pharmacology* / Q000627:therapeutic use; D001692:Biological Transport; D005972:Glutaminase / Q000235:genetics / Q000378:metabolism*; D005973:Glutamine / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D007422:Intestines / Q000187:drug effects* / Q000473:pathology; D008070:Lipopolysaccharides; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D018805:Sepsis / Q000188:drug therapy* / Q000378:metabolism
chemical_list: D026921:Amino Acid Transport Systems, Neutral; D008070:Lipopolysaccharides; D010936:Plant Extracts; D012333:RNA, Messenger; C520324:SLC6A19 protein, rat; D001599:Berberine; D005973:Glutamine; D005972:Glutaminase