title: Analysis of the expression pattern of the BCL11B gene and its relatives in patients with T-cell acute lymphoblastic leukemia.
abstract: BACKGROUND
  In a human T-cell acute lymphoblastic leukemia (T-ALL) cell line (Molt-4), siRNA-mediated suppression of BCL11B expression was shown to inhibit proliferation and induce apoptosis, functions which may be related to genes involved in apoptosis (such as TNFSF10 and BCL2L1) and TGF-β pathways (such as SPP1and CREBBP).
  
  
  METHODS
  The expression levels of the above mentioned genes and their correlation with the BCL11B gene were analyzed in patients with T-ALL using the TaqMan and SYBR Green I real-time polymerase chain reaction technique.
  
  
  RESULTS
  Expression levels of BCL11B, BCL2L1, and CREBBP mRNA in T-ALL patients were significantly higher than those from healthy controls (P<0.05). In T-ALL patients, the BCL11B expression level was negatively correlated with the BCL2L1 expression level (rs=-0.700; P<0.05), and positively correlated with the SPP1 expression level (rs=0.683; P<0.05). In healthy controls, the BCL11B expression level did not correlate with the TNFSF10, BCL2L1, SPP1, or CREBBP expression levels.
  
  
  CONCLUSIONS
  Over-expression of BCL11B might play a role in anti-apoptosis in T-ALL cells through up-regulation of its downstream genes BCL2L1 and CREBBP.
authors.affiliation: Institute of Hematology, Medical College, Jinan University, and Department of Hematology, Guangdong General Hospital, Guangzhou, 510632, PR China.
authors.forename: Xin
authors.initials: X
authors.lastname: Huang
authors.forename: Shaohua
authors.initials: S
authors.lastname: Chen
authors.forename: Qi
authors.initials: Q
authors.lastname: Shen
authors.forename: Lijian
authors.initials: L
authors.lastname: Yang
authors.forename: Bo
authors.initials: B
authors.lastname: Li
authors.forename: Liye
authors.initials: L
authors.lastname: Zhong
authors.forename: Suxia
authors.initials: S
authors.lastname: Geng
authors.forename: Xin
authors.initials: X
authors.lastname: Du
authors.forename: Yangqiu
authors.initials: Y
authors.lastname: Li
mesh_terms: D000293:Adolescent; D000328:Adult; D050882:CREB-Binding Protein / Q000096:biosynthesis* / Q000235:genetics; D002648:Child; D015870:Gene Expression; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D006801:Humans; D054218:Precursor T-Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics*; D012097:Repressor Proteins / Q000096:biosynthesis* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D025521:Tumor Suppressor Proteins / Q000096:biosynthesis* / Q000235:genetics; D055815:Young Adult; D051020:bcl-X Protein / Q000096:biosynthesis* / Q000235:genetics
chemical_list: C479740:BCL11B protein, human; C494811:BCL2L1 protein, human; D012097:Repressor Proteins; D025521:Tumor Suppressor Proteins; D051020:bcl-X Protein; D050882:CREB-Binding Protein; C494363:CREBBP protein, human