title: [Serum-free medium for suspension culture of recombinant Chinese hamster ovary (11G-S) cells].
abstract: With suspension adapted recombinant Chinese hamster ovary (CHO) cell lines 11G-S expressing human pro-urokinase (pro-UK) as the object of study, a serum-free medium for the cultivation of recombinant CHO cells in suspension was formulated by using Plackett-Burman design and response surface methodology. The two-level Plackett-Burman design was used to evaluate the effect of 10 medium supplements on the growth of the 11G-S cells in suspension culture. Among the 10 medium supplements, insulin, transferrin, and putrescine were identified as the most significant factors (P < 0.05). The response surface methodology with three factors and three levels was used to determine the optimal levels of these factors. And a serum-free medium, SFM-CHO-S for recombinant CHO cells suspension culture was formulated. The maximum cell density of 11G-S cells in SFM-CHO-S in suspension batch culture reached 4.12 x 10(6) cells/mL with a maximum pro-UK activity at 5614 IU/mL, which was superior to the commercial serum-free medium for recombinant CHO cells.
authors.affiliation: Institute of Biotechnology, Academy of Military Medical Sciences, Beijing 100071, China.
authors.forename: Xingmao
authors.initials: X
authors.lastname: Liu
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
authors.forename: Lingling
authors.initials: L
authors.lastname: Ye
authors.forename: Shichong
authors.initials: S
authors.lastname: Li
authors.forename: Benchuan
authors.initials: B
authors.lastname: Wu
authors.forename: Haitao
authors.initials: H
authors.lastname: Wang
authors.forename: Jing
authors.initials: J
authors.lastname: Xie
authors.forename: Zhaolie
authors.initials: Z
authors.lastname: Chen
mesh_terms: D000818:Animals; D016466:CHO Cells; D018929:Cell Culture Techniques / Q000379:methods*; D006224:Cricetinae; D003412:Cricetulus; D016895:Culture Media, Serum-Free; D005818:Genetic Engineering; D007328:Insulin / Q000494:pharmacology; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D014168:Transferrin / Q000494:pharmacology; D014568:Urokinase-Type Plasminogen Activator / Q000096:biosynthesis / Q000235:genetics
chemical_list: D016895:Culture Media, Serum-Free; D007328:Insulin; D011994:Recombinant Proteins; D014168:Transferrin; D014568:Urokinase-Type Plasminogen Activator