title: Destabilase-lysozyme of medicinal leech. Multifunctionality of recombinant protein.
abstract: Preparation and purification of a recombinant protein are described along with characteristics of its specific (for ε-(γ-Glu)-Lys and D-dimer substrates) and nonspecific (for L-γ-Glu-pNA) isopeptidase activities; the absence of peptidase function for α-(α-Glu)-Lys substrate is noted. It is shown that the protein exhibits muramidase (cell walls of Micrococcus lysodeikticus) and specific glycosidase activities. The latter was determined towards the fluorogenic substrate 4-methylumbelliferyl-tetra-N-acetyl-β-chitotetraoxide. Antimicrobial activity of recombinant destabilase-lysozyme protein (recDest-Lys) and its 11-membered amphipathic peptide was revealed towards cells of the strict anaerobic Archaean Methanosarcina barkeri, whose cell walls contain no murein. Possible mechanisms of the effect of recDest-Lys on these cells are discussed.
authors.affiliation: Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 119997, Russia. lz@ibch.ru
authors.forename: L L
authors.initials: LL
authors.lastname: Zavalova
authors.forename: V N
authors.initials: VN
authors.lastname: Lazarev
authors.forename: S A
authors.initials: SA
authors.lastname: Levitsky
authors.forename: T G
authors.initials: TG
authors.lastname: Yudina
authors.forename: I P
authors.initials: IP
authors.lastname: Baskova
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology; D010450:Endopeptidases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000737:chemistry; D007865:Leeches / Q000201:enzymology*; D046529:Microscopy, Electron, Transmission; D009113:Muramidase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D013379:Substrate Specificity
chemical_list: D000890:Anti-Infective Agents; D005456:Fluorescent Dyes; D011994:Recombinant Proteins; D009113:Muramidase; D010450:Endopeptidases; C045348:fibrin destabilase