title: Characterization of a F-box gene up-regulated by phytohormones and upon biotic and abiotic stresses in grapevine.
abstract: F-box proteins are key components of the ubiquitin (Ub)/26S proteasome pathway that mediates selective degradation of regulatory proteins involved in a wide variety of cellular processes affecting eukaryotic cells. In plants, F-box genes form one of the largest multigene superfamilies and control many important biological functions. Among the F-box genes characterized to date only few have been involved in the regulation of plant defense responses. Moreover, no F-box genes have been studied and characterized in grapevine. Using a differential display approach we isolated a F-box gene (BIG-24.1), which is up-regulated during Botrytis cinerea infection of grapevine leaves. BIG-24.1 encodes a polypeptide of 386 amino acids with a conserved F-box domain in the N-terminus region and a kelch domain. By investigating expression profiles of BIG-24.1, we show that the gene expression is strongly stimulated in B. cinerea infected berries and in grapevine cells challenged by MAMP rhamnolipids, a non-host bacterium and an endophytic rhizobacterium. The gene is also strongly induced by abiotic stresses including UV-C and wounding or by salicylic acid, methyl-jasmonate, ethylene and abscisic acid that are known to be involved in defense signalling pathways. In addition, sequence analysis of the BIG-24.1 promoter revealed the presence of several regulatory elements involved in the activation of plant defense responses.
authors.affiliation: URVVC-SE-EA 2069, Stress, Défenses et Reproduction des Plantes, Université de Reims Champagne-Ardenne, BP 1039, 51687 Reims cedex 2, France.
authors.forename: Sandra
authors.initials: S
authors.lastname: Paquis
authors.forename: Florence
authors.initials: F
authors.lastname: Mazeyrat-Gourbeyre
authors.forename: Olivier
authors.initials: O
authors.lastname: Fernandez
authors.forename: Jérôme
authors.initials: J
authors.lastname: Crouzet
authors.forename: Christophe
authors.initials: C
authors.lastname: Clément
authors.forename: Fabienne
authors.initials: F
authors.lastname: Baillieul
authors.forename: Stéphan
authors.initials: S
authors.lastname: Dorey
mesh_terms: D000595:Amino Acid Sequence; D020171:Botrytis / Q000378:metabolism / Q000472:pathogenicity; D044783:F-Box Proteins / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D008969:Molecular Sequence Data; D010937:Plant Growth Regulators / Q000494:pharmacology*; D018515:Plant Leaves / Q000378:metabolism / Q000382:microbiology* / Q000502:physiology*; D011401:Promoter Regions, Genetic; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013312:Stress, Physiological; D015854:Up-Regulation / Q000187:drug effects; D027843:Vitis / Q000033:anatomy & histology / Q000235:genetics* / Q000378:metabolism / Q000382:microbiology
chemical_list: D044783:F-Box Proteins; D010937:Plant Growth Regulators