title: Combination of PDT and inhibitor treatment affects melanoma cells and spares keratinocytes.
abstract: Photodynamic therapy (PDT) is a potential tool in cancer treatment. Today this therapy is established among others for the treatment of nonmelanoma skin cancer. However, the more dangerous skin cancer--the melanoma--still has to be removed by surgery. Therefore, we investigated the effects of PDT and additional administration of heme oxygenase I (HO-I) and poly(ADP-ribose) polymerase (PARP) inhibitors on the treatment of melanoma cells in comparison to nonmalignant keratinocytes. Therefore, cocultures were established with WM451LU melanoma cells and HaCaT keratinocytes. In the coculture some 65% melanoma cells and 35% HaCaT cells were present before PDT, whereas after PDT the proportion was 41% melanoma cells and 59% HaCaT cells. Combination of both inhibitors improves these results to only 16% melanoma cells and 84% HaCaT cells. PDT is, therefore, a potent skin cancer treatment, which might also be interesting for melanoma treatment. The cytotoxic effects of PDT are largely mediated by ROS. Addition of HO-I and PARP inhibitors could improve the efficiency of photodynamic treatment.
authors.affiliation: Department of Nutritional Toxicology, Institute of Nutrition, Friedrich Schiller University Jena, Jena, Germany.
authors.forename: Marc
authors.initials: M
authors.lastname: Kästle
authors.forename: Stefanie
authors.initials: S
authors.lastname: Grimm
authors.forename: Rena
authors.initials: R
authors.lastname: Nagel
authors.forename: Nicolle
authors.initials: N
authors.lastname: Breusing
authors.forename: Tilman
authors.initials: T
authors.lastname: Grune
mesh_terms: D017209:Apoptosis / Q000187:drug effects / Q000528:radiation effects; D002478:Cells, Cultured; D018920:Coculture Techniques; D003131:Combined Modality Therapy; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D051547:Heme Oxygenase-1 / Q000037:antagonists & inhibitors / Q000378:metabolism; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects* / Q000528:radiation effects*; D008027:Light; D008545:Melanoma / Q000378:metabolism / Q000556:secondary / Q000628:therapy*; D010778:Photochemotherapy; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D004791:Enzyme Inhibitors; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D017382:Reactive Oxygen Species; D051547:Heme Oxygenase-1; D011065:Poly(ADP-ribose) Polymerases