title: [High-performance liquid chromatography for determination of psoralene, bergapten and apigenin in Ficus hirta Vahl].
abstract: OBJECTIVE
  To establish a high-performance liquid chromatography (HPLC)-based method for determining the contents of psoralene, bergapten and apigenin in Ficus hirta Vahl.
  
  
  METHODS
  A Hypersil C18 column (250 mm × 4.6 mm, 5 µm) was used with the mobile phase of methanol-water (60:40), flow rate of 1.0 ml/min, detection wavelength of 268.7 nm and column temperature of 30 degrees celsius.
  
  
  RESULTS
  The calibration curve was linear within the range of 10.0-30.0 µg/ml for psoralene (r=0.9998), 15.0-45.0 µg/ml for bergapten (r=0.9998) and 5.0-15.0 µg/ml for apigenin (r=0.9992). The average recovery of psoralene was 99.7% (RSD=1.99%), that of bergapten was 99.9% (RSD=1.71%) and that of apigenin was 100.3% (RSD=1.78%).
  
  
  CONCLUSIONS
  The method is simple, economic and accurate with good reproducibility for the contents of psoralene, bergapten and apigenin.
authors.affiliation: Guangdong Food and Drug Vocational College, Guangzhou 510520, China. ngyj@gdyzy.edu.cn
authors.forename: Yan-jun
authors.initials: YJ
authors.lastname: Yang
authors.forename: Jun
authors.initials: J
authors.lastname: Dai
mesh_terms: D000078223:5-Methoxypsoralen; D047310:Apigenin / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D005363:Ficusin / Q000032:analysis*; D008730:Methoxsalen / Q000031:analogs & derivatives* / Q000032:analysis
chemical_list: D004365:Drugs, Chinese Herbal; D000078223:5-Methoxypsoralen; D047310:Apigenin; D005363:Ficusin; D008730:Methoxsalen