title: Liquid chromatography-tandem mass spectrometry method for the determination of meloxicam and its metabolite 5-carboxymeloxicam in human plasma.
abstract: BACKGROUND
  To develop and validate a rapid, sensitive and selective liquid chromatography-electrospray ionization mass spectrometric method for the determination of meloxicam and its metabolite 5-carboxymeloxicam in human plasma.
  
  
  RESULTS
  A liquid extraction method was chosen for sample clean-up. Meloxicam, 5-carboxymeloxicam and isoxicam (internal standard) were analyzed on an XBridge™ C18 column with 65% methanol in 10 mM ammonium formate (pH 3.0) and detected in selected reaction monitoring mode. The standard curves were linear over the concentration range 10-2500 ng/ml for meloxicam and 2-100 ng/ml for 5-carboxymeloxicam. Matrix effects were practically absent.
  
  
  CONCLUSIONS
  This method has been successfully applied to the pharmacokinetic study of meloxicam and 5-carboxymeloxicam after oral administration of meloxicam (15 mg) to 30 volunteers.
authors.affiliation: Drug Metabolism and Bioanalysis Laboratory, College of Pharmacy, Wonkwang University, Iksan 570-749, Korea. hslee@wku.ac.kr
authors.forename: Hye Won
authors.initials: HW
authors.lastname: Lee
authors.forename: Hye Young
authors.initials: HY
authors.lastname: Ji
authors.forename: Hoi Yun
authors.initials: HY
authors.lastname: Kim
authors.forename: Kang Choon
authors.initials: KC
authors.lastname: Lee
authors.forename: Hye Suk
authors.initials: HS
authors.lastname: Lee
mesh_terms: D000284:Administration, Oral; D019540:Area Under Curve; D002138:Calibration; D002853:Chromatography, Liquid; D016861:Cyclooxygenase Inhibitors / Q000097:blood* / Q000493:pharmacokinetics; D004355:Drug Stability; D006801:Humans; D008297:Male; D000077239:Meloxicam; D010894:Piroxicam / Q000031:analogs & derivatives / Q000032:analysis; D012015:Reference Standards; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry; D013843:Thiazines / Q000008:administration & dosage / Q000097:blood* / Q000378:metabolism / Q000493:pharmacokinetics; D013844:Thiazoles / Q000008:administration & dosage / Q000097:blood* / Q000378:metabolism / Q000493:pharmacokinetics
chemical_list: D016861:Cyclooxygenase Inhibitors; D013843:Thiazines; D013844:Thiazoles; D010894:Piroxicam; C013580:isoxicam; C558503:5-carboxymeloxicam; D000077239:Meloxicam