title: Effect of storage temperature and length on fatty acid composition of fingertip blood collected on filter paper.
abstract: We have compared the fatty acids of the capillary and venous whole blood samples collected on the commercially developed blood collection paper and standard grade filter paper extracted by either the direct methylation or conventional method (including various blood lipids fractions). Also, reproducibility of fatty acids extracted from dried blood on the filter paper after storing at room temperature up to 2 months and at 4°C up to 6 months was assessed. In conclusion, the direct methylation of fingertip blood collected on both brand of papers produced fatty acids that reflected venous blood fatty acids extracted by the conventional method. Of the eight fatty acids evaluated, capillary DHA showed the strongest correlation with DHA of the venous whole lipids as well as various lipid fractions of the plasma and red cells. However, a prolonged storage of blood samples at 4°C had deleterious effect on the qualitative value of fatty acids, especially DHA.
authors.affiliation: Institute of Brain Chemistry and Human Nutrition, London Metropolitan University, 166-220 Holloway Road, London N7 8DB, UK. y.min@londonmet.ac.uk
authors.forename: Yoeju
authors.initials: Y
authors.lastname: Min
authors.forename: Kebreab
authors.initials: K
authors.lastname: Ghebremeskel
authors.forename: Julia
authors.initials: J
authors.lastname: Geppert
authors.forename: Fawad
authors.initials: F
authors.lastname: Khalil
mesh_terms: D001800:Blood Specimen Collection / Q000379:methods*; D002196:Capillaries; D003080:Cold Temperature; D005227:Fatty Acids / Q000097:blood*; D005260:Female; D005385:Fingers / Q000098:blood supply*; D005542:Forearm / Q000098:blood supply; D006801:Humans; D008297:Male; D010209:Paper
chemical_list: D005227:Fatty Acids