title: Effect of vitamin E on oxidative stress in the contralateral testis of neonatal and pubertal hemicastrated rats.
abstract: OBJECTIVE
  To evaluate whether the antioxidant vitamin E can prevent the harmful effects of reactive oxidative stress (ROS) that occur during compensatory testicular hypertrophy (CTH).
  
  
  METHODS
  Thirty Sprague-Dawley rats were divided into six equal groups: neonatal hemicastrated vitamin E (NH_Vit E/NH) and sham surgical controls (NC), and pubertal hemicastrated vitamin E (PH_Vit E/PH) and sham surgical controls (PC). Vitamin E was administered orally to the NH_Vit E and PH_Vit E groups three times a week from week 3-12 prior to sacrifice. Antioxidant enzymes were measured in testis samples from each animal.
  
  
  RESULTS
  Differences in superoxide dismutase activity were observed between the NH (21.04 ± 0.48) and NH_Vit E (22.62 ± 0.64) groups (P = 0.008); the PH (20.59 ± 0.11) and PC (20.91 ± 0.20) groups (P = 0.032); and the PH (20.59 ± 0.11) and PH_Vit E (22.32 ± 1.01) groups (P = 0.008). Thiobarbituric acid-reactive substance in the PH and PH_Vit E groups was 0.097 ± 0.022 and 0.036 ± 0.004 (P = 0.008), respectively; and in the NH and NH_Vit E groups it was 0.135 ± 0.02 and 0.039 ± 0.003 (P = 0.008), respectively.
  
  
  CONCLUSIONS
  These results suggest that CTH is not associated with reducing oxidative injury, nor does it prevent ROS-induced cell damage. However, administration of vitamin E does reduce oxidative injury and prevent ROS-induced cell damage in a hemicastrated rat model.
authors.affiliation: Department of Urology, Yonsei University College of Medicine, Urological Science Institute, Brain Korea 21 Project for Medical Science, 134, Shinchon-dong, Seudaemun-gu, Seoul 120-752, South Korea.
authors.forename: Woong Kyu
authors.initials: WK
authors.lastname: Han
authors.forename: Mei Hua
authors.initials: MH
authors.lastname: Jin
authors.forename: Sang Won
authors.initials: SW
authors.lastname: Han
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000831:Animals, Newborn; D000975:Antioxidants / Q000494:pharmacology; D002374:Catalase / Q000187:drug effects / Q000378:metabolism; D004195:Disease Models, Animal; D005980:Glutathione Reductase / Q000187:drug effects / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D009919:Orchiectomy / Q000379:methods*; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012016:Reference Values; D012680:Sensitivity and Specificity; D012741:Sexual Maturation; D013482:Superoxide Dismutase / Q000187:drug effects / Q000378:metabolism*; D014810:Vitamin E / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D014810:Vitamin E; D002374:Catalase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase