title: New method based on capillary electrophoresis with laser-induced fluorescence detection (CE-LIF) to monitor interaction between nanoparticles and the amyloid-β peptide.
abstract: A novel application of capillary electrophoresis with laser-induced fluorescence detection (CE-LIF) was proposed to efficiently detect and monitor the interaction between polymeric nanoparticles and the β-Amyloid peptide (Aβ(1-42)), a biomarker for Alzheimer's Disease (AD), at concentrations close to physiological conditions. The CE-LIF method allowed the interaction between PEGylated poly(alkyl cyanoacrylate) nanoparticles (NPs) and the soluble Aβ(1-42) peptide monomers to be highlighted. These results were confirmed by surface plasmon resonance (SPR) and confocal laser scanning microscopy (CLSM). Whereas SPR showed an interaction between the NPs and the Aβ(1-42) peptide, CLSM allowed the formation of large aggregates/assemblies at high NP and peptide concentrations to be visualized. All these results suggested that these nanoparticles could bind the Aβ(1-42) peptide and influence its aggregation kinetics. Interestingly, the non-PEGylated poly(alkyl cyanoacrylate) NPs did not alter the aggregation kinetics of the Aβ(1-42) peptide, thus emphasizing the high level of discrimination of the CE-LIF method with respect to NPs.
authors.affiliation: Laboratoire de Physico-Chimie, Pharmacotechnie et Biopharmacie, UMR CNRS 8612, Univ Paris-Sud 11, Faculté de Pharmacie, 5 rue Jean-Baptiste Clément, F-92296 Chatenay-Malabry, France.
authors.forename: Davide
authors.initials: D
authors.lastname: Brambilla
authors.forename: Romain
authors.initials: R
authors.lastname: Verpillot
authors.forename: Myriam
authors.initials: M
authors.lastname: Taverna
authors.forename: Line
authors.initials: L
authors.lastname: De Kimpe
authors.forename: Benjamin
authors.initials: B
authors.lastname: Le Droumaguet
authors.forename: Julien
authors.initials: J
authors.lastname: Nicolas
authors.forename: Mara
authors.initials: M
authors.lastname: Canovi
authors.forename: Marco
authors.initials: M
authors.lastname: Gobbi
authors.forename: Francesco
authors.initials: F
authors.lastname: Mantegazza
authors.forename: Mario
authors.initials: M
authors.lastname: Salmona
authors.forename: Valérie
authors.initials: V
authors.lastname: Nicolas
authors.forename: Wiep
authors.initials: W
authors.lastname: Scheper
authors.forename: Patrick
authors.initials: P
authors.lastname: Couvreur
authors.forename: Karine
authors.initials: K
authors.lastname: Andrieux
mesh_terms: D016229:Amyloid beta-Peptides / Q000378:metabolism*; D019075:Electrophoresis, Capillary / Q000379:methods*; D005470:Fluorometry / Q000379:methods*; D007700:Kinetics; D007834:Lasers; D008722:Methods; D053758:Nanoparticles / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D011485:Protein Binding; D055503:Protein Multimerization
chemical_list: D016229:Amyloid beta-Peptides; D011108:Polymers