title: Efficient and scalable serial extraction of DNA and RNA from frozen tissue samples.
abstract: Advances in cancer genomics have created a demand for scalable sample processing. We here present a process for serial extraction of nucleic acids from the same frozen tissue sample based on magnetic silica particles. The process is automation friendly with high recoveries of pure DNA and RNA suitable for analysis.
authors.affiliation: Department of Genetics and Pathology, Uppsala University, SE-751 85 Uppsala, Sweden.
authors.forename: Lucy
authors.initials: L
authors.lastname: Mathot
authors.forename: Monica
authors.initials: M
authors.lastname: Lindman
authors.forename: Tobias
authors.initials: T
authors.lastname: Sjöblom
mesh_terms: D053000:Analytic Sample Preparation Methods / Q000379:methods*; D001331:Automation; D004247:DNA / Q000235:genetics / Q000302:isolation & purification*; D005615:Freezing; D023281:Genomics / Q000379:methods*; D006801:Humans; D008280:Magnetics; D009369:Neoplasms / Q000235:genetics*; D016612:Paraffin Embedding; D010316:Particle Size; D012313:RNA / Q000235:genetics / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012822:Silicon Dioxide / Q000737:chemistry; D013499:Surface Properties; D046888:Tissue Array Analysis
chemical_list: D012313:RNA; D012822:Silicon Dioxide; D004247:DNA