title: Crystal structure of a non-neutralizing antibody to the HIV-1 gp41 membrane-proximal external region.
abstract: The monoclonal antibody 13H11 shares part of its epitope in the HIV-1 gp41 membrane-proximal external region (MPER) with the rare, broadly neutralizing human antibody 2F5. Although 13H11 partially cross-blocked 2F5 binding, 13H11 is non-neutralizing and does not block 2F5 neutralization. We show that unlike 2F5, 13H11 binds to a well-defined helical MPER structure that is consistent with the structure of gp41 in a post-fusion six-helix bundle conformation.
authors.affiliation: Duke Human Vaccine Institute, Duke University School of Medicine, Durham, North Carolina, USA. nathan.nicely@duke.edu
authors.forename: Nathan I
authors.initials: NI
authors.lastname: Nicely
authors.forename: S Moses
authors.initials: SM
authors.lastname: Dennison
authors.forename: Leonard
authors.initials: L
authors.lastname: Spicer
authors.forename: Richard M
authors.initials: RM
authors.lastname: Scearce
authors.forename: Garnett
authors.initials: G
authors.lastname: Kelsoe
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Ueda
authors.forename: Haiyan
authors.initials: H
authors.lastname: Chen
authors.forename: Hua-Xin
authors.initials: HX
authors.lastname: Liao
authors.forename: S Munir
authors.initials: SM
authors.lastname: Alam
authors.forename: Barton F
authors.initials: BF
authors.lastname: Haynes
mesh_terms: D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000276:immunology; D018360:Crystallography, X-Ray; D015483:HIV Antibodies / Q000737:chemistry* / Q000276:immunology; D015700:HIV Envelope Protein gp41 / Q000737:chemistry* / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000737:chemistry; D008958:Models, Molecular; D008969:Molecular Sequence Data
chemical_list: D000911:Antibodies, Monoclonal; D015483:HIV Antibodies; D015700:HIV Envelope Protein gp41; D007140:Immunoglobulin Fab Fragments; C514787:gp41 protein, Human immunodeficiency virus 1