title: Comparative study of subcutaneous tissue responses to a novel root-end filling material and white and grey mineral trioxide aggregate.
abstract: OBJECTIVE
  To compare the subcutaneous tissue response to grey mineral trioxide aggregate (GMTA), white mineral trioxide aggregate (WMTA) and a new experimental cement (calcium enriched cement, CEM).
  
  
  METHODS
  Thirty-six Wistar male albino rats each received three implants, containing one of the tested materials, and an empty tube as a control. Seven, 30 and 60 days after implantation, the animals were sacrificed. After histological preparation and H&E staining, the specimens were evaluated for capsule thickness, necrosis, and for the type, the severity, and the extent of inflammation. Kruskal Wallis and Chi-square tests were used for data analysis.
  
  
  RESULTS
  After 1 week, CEM produced no necrosis compared to both types of WMTA and GMTA (P = 0.007). After 30 days, GMTA specimens had significantly less inflammation compared with WMTA and CEM (P = 0.011). After 60 days, less inflammation was associated with CEM specimens (P = 0.0001) compared to the other materials. Dystrophic calcifications in the connective tissue adjacent to all experimental material were detected.
  
  
  CONCLUSIONS
  Histological observation illustrated that all materials were well tolerated by the subcutaneous tissues.
authors.affiliation: Oral and Dental Diseases Research Center, School of Dentistry, Kerman University of Medical Sciences, Kerman, Iran.
authors.forename: M
authors.initials: M
authors.lastname: Parirokh
authors.forename: B
authors.initials: B
authors.lastname: Mirsoltani
authors.forename: M
authors.initials: M
authors.lastname: Raoof
authors.forename: H
authors.initials: H
authors.lastname: Tabrizchi
authors.forename: A A
authors.initials: AA
authors.lastname: Haghdoost
mesh_terms: D017607:Aluminum Compounds / Q000633:toxicity; D000818:Animals; D017610:Calcium Compounds / Q000633:toxicity; D003738:Dental Cements / Q000633:toxicity*; D004338:Drug Combinations; D008297:Male; D008422:Materials Testing; D010087:Oxides / Q000633:toxicity; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012387:Root Canal Filling Materials / Q000633:toxicity*; D017640:Silicates / Q000633:toxicity; D040521:Subcutaneous Tissue / Q000187:drug effects
chemical_list: D017607:Aluminum Compounds; D017610:Calcium Compounds; D003738:Dental Cements; D004338:Drug Combinations; D010087:Oxides; D012387:Root Canal Filling Materials; D017640:Silicates; C086631:mineral trioxide aggregate; C016538:lime