title: Relationship between endogenous protein disulfide isomerase family proteins and glutenin macropolymer.
abstract: The effects of endogenous protein disulfide isomerase (PDI) family proteins on the properties of gluten proteins in dough during breadmaking were determined using bacitracin, an inhibitor of PDI. Bread loaf volume in the presence of bacitracin was increased to 118% of that in the absence of bacitracin. The addition of bacitracin caused a decrease in the extension tolerance of the dough. The amount of sodium dodecyl sulfate (SDS)-insoluble glutenin macropolymer (GMP) in dough decreased to approximately 70% of that in flour during the 20 min of mixing for doughmaking. The addition of bacitracin to dough caused a dramatic GMP decrease, corresponding to ∼20-30% of that in flour during the 20 min of mixing. The decrease in GMP was compensated by an increase in SDS-soluble glutenin polymer. Taken together, these results suggest that the endogenous PDI family proteins in flour suppress the depolymerization of GMP during dough mixing.
authors.affiliation: Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan.
authors.forename: Akie
authors.initials: A
authors.lastname: Koh
authors.forename: Kimio
authors.initials: K
authors.lastname: Nishimura
authors.forename: Reiko
authors.initials: R
authors.lastname: Urade
mesh_terms: D001414:Bacitracin / Q000494:pharmacology; D001939:Bread / Q000032:analysis; D003296:Cooking; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005433:Flour / Q000032:analysis; D005983:Glutens / Q000737:chemistry*; D005810:Multigene Family; D010940:Plant Proteins / Q000032:analysis* / Q000037:antagonists & inhibitors; D058105:Polymerization; D019704:Protein Disulfide-Isomerases / Q000032:analysis* / Q000037:antagonists & inhibitors; D014908:Triticum / Q000737:chemistry / Q000201:enzymology*
chemical_list: D004791:Enzyme Inhibitors; D010940:Plant Proteins; D001414:Bacitracin; D005983:Glutens; D019704:Protein Disulfide-Isomerases; C037302:glutenin