title: Light-activated regulation of cofilin dynamics using a photocaged hydrogen peroxide generator.
abstract: Hydrogen peroxide (H2O2) can exert diverse signaling and stress responses within living systems depending on its spatial and temporal dynamics. Here we report a new small-molecule probe for producing H2O2 on demand upon photoactivation and its application for optical regulation of cofilin-actin rod formation in living cells. This chemical method offers many potential opportunities for dissecting biological roles for H2O2 as well as remote control of cell behavior via H2O2-mediated pathways.
authors.affiliation: Department of Chemistry, University of California, Berkeley, California 94720, USA.
authors.forename: Evan W
authors.initials: EW
authors.lastname: Miller
authors.forename: Nicolas
authors.initials: N
authors.lastname: Taulet
authors.forename: Carl S
authors.initials: CS
authors.lastname: Onak
authors.forename: Elizabeth J
authors.initials: EJ
authors.lastname: New
authors.forename: Julie K
authors.initials: JK
authors.lastname: Lanselle
authors.forename: Gillian S
authors.initials: GS
authors.lastname: Smelick
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Chang
mesh_terms: D051339:Actin Depolymerizing Factors / Q000378:metabolism*; D002470:Cell Survival; D006367:HeLa Cells; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism*; D008027:Light; D015335:Molecular Probes / Q000138:chemical synthesis / Q000378:metabolism*; D010782:Photolysis
chemical_list: D051339:Actin Depolymerizing Factors; D015335:Molecular Probes; D006861:Hydrogen Peroxide