title: Isolation of human adipose-derived stem cells from lipoaspirates.
abstract: Adipose tissue is as an abundant and accessible source of stem cells with multipotent properties suitable for tissue engineering and regenerative medical applications. Here, we describe methods from our own laboratory and the literature for the isolation and expansion of adipose-derived stem cells (ASCs). We present a large-scale procedure suitable for processing >100-ml volumes of lipoaspirate tissue specimens by collagenase digestion and a related procedure suitable for processing adipose tissue aspirates without digestion.
authors.affiliation: Stem Cell Biology Laboratory, Pennington Biomedical Research Center, Louisiana State University System, Baton Rouge, LA, USA.
authors.forename: Gang
authors.initials: G
authors.lastname: Yu
authors.forename: Z Elizabeth
authors.initials: ZE
authors.lastname: Floyd
authors.forename: Xiying
authors.initials: X
authors.lastname: Wu
authors.forename: Yuan-Di C
authors.initials: YD
authors.lastname: Halvorsen
authors.forename: Jeffrey M
authors.initials: JM
authors.lastname: Gimble
mesh_terms: D017667:Adipocytes / Q000166:cytology; D000273:Adipose Tissue / Q000166:cytology*; D002454:Cell Differentiation; D002469:Cell Separation / Q000379:methods*; D015925:Cryopreservation; D005434:Flow Cytometry; D006801:Humans; D015187:Lipectomy; D013194:Staining and Labeling; D013234:Stem Cells / Q000166:cytology*