title: Association between oxidative stress and masked hypertension in a multi-ethnic population of obese children and adolescents.
abstract: OBJECTIVE
  To evaluate whether oxidative stress is correlated with adiposity, obesity-related metabolic abnormalities, and ambulatory blood pressure (ABP) in a multi-ethnic pediatric population.
  
  
  METHODS
  We conducted a prospective study enrolling 42 obese children (age, 12.8 ± 2.4 years) and 34 non-obese children (age, 11.8 ± 3.4 years). We measured urine 8-isoprostane and hydrogen peroxide (markers of oxidative stress) in both obese and non-obese groups. In the obese group, we measured the 24-hour ABP and obtained an oral glucose tolerance test, lipid panel, interleukin-6, and tumor necrosis factor-α.
  
  
  RESULTS
  8-isoprostane and hydrogen peroxide were correlated with body mass index standard deviation score and waist circumference. The mean 8-isoprostane and hydrogen peroxide levels of the obese group were higher than those of the non-obese group. In the subset of obese subjects who underwent ABP monitoring, 8-isoprostane was correlated with mean 24-hour systolic blood pressure: within the obese group, 8-isoprostane was higher in obese children with elevated mean 24-hour systolic blood pressure.
  
  
  CONCLUSIONS
  Our findings provide evidence of a significant correlation between oxidative stress, adiposity, and blood pressure in children. Longitudinal studies in a larger population sample are needed to validate the association between elevated urine 8-isoprostane level and cardiovascular risk factors in an obese pediatric population.
authors.affiliation: Section of Endocrinology and Diabetes, St. Christopher's Hospital for Children, Department of Pediatrics, Drexel University College of Medicine, Philadelphia, PA, USA.
authors.forename: Vlady
authors.initials: V
authors.lastname: Ostrow
authors.forename: Shufang
authors.initials: S
authors.lastname: Wu
authors.forename: Alexandra
authors.initials: A
authors.lastname: Aguilar
authors.forename: Robert
authors.initials: R
authors.lastname: Bonner
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Suarez
authors.forename: Francesco
authors.initials: F
authors.lastname: De Luca
mesh_terms: D000293:Adolescent; D002648:Child; D003430:Cross-Sectional Studies; D015237:Dinoprost / Q000031:analogs & derivatives / Q000652:urine; D050171:Dyslipidemias / Q000453:epidemiology / Q000208:ethnology; D005260:Female; D006801:Humans; D006861:Hydrogen Peroxide / Q000652:urine; D006973:Hypertension / Q000208:ethnology / Q000503:physiopathology*; D015850:Interleukin-6 / Q000097:blood; D008297:Male; D009765:Obesity / Q000453:epidemiology / Q000208:ethnology / Q000503:physiopathology* / Q000652:urine; D018384:Oxidative Stress / Q000502:physiology*; D011446:Prospective Studies; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; C075750:8-epi-prostaglandin F2alpha; D015237:Dinoprost; D006861:Hydrogen Peroxide