title: Dictyostelium dynamin B modulates cytoskeletal structures and membranous organelles.
abstract: Dictyostelium discoideum cells produce five dynamin family proteins. Here, we show that dynamin B is the only member of this group of proteins that is initially produced as a preprotein and requires processing by mitochondrial proteases for formation of the mature protein. Our results show that dynamin B-depletion affects many aspects of cell motility, cell-cell and cell-surface adhesion, resistance to osmotic shock, and fatty acid metabolism. The mature form of dynamin B mediates a wide range and unique combination of functions. Dynamin B affects events at the plasma membrane, peroxisomes, the contractile vacuole system, components of the actin-based cytoskeleton, and cell adhesion sites. The modulating effect of dynamin B on the activity of the contractile vacuole system is unique for the Dictyostelium system. Other functions displayed by dynamin B are commonly associated with either classical dynamins or dynamin-related proteins.
authors.affiliation: Institut für Biophysikalische Chemie, Medizinische Hochschule Hannover, Carl-Neuberg-Straße, Germany.
authors.forename: Amrita
authors.initials: A
authors.lastname: Rai
authors.forename: Heike
authors.initials: H
authors.lastname: Nöthe
authors.forename: Nikolay
authors.initials: N
authors.lastname: Tzvetkov
authors.forename: Elena
authors.initials: E
authors.lastname: Korenbaum
authors.forename: Dietmar J
authors.initials: DJ
authors.lastname: Manstein
mesh_terms: D000595:Amino Acid Sequence; D002448:Cell Adhesion; D002462:Cell Membrane / Q000378:metabolism / Q000502:physiology; D003599:Cytoskeleton / Q000502:physiology*; D004023:Dictyostelium / Q000201:enzymology*; D034281:Dynamins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism; D055786:Gene Knockout Techniques; D008928:Mitochondria / Q000201:enzymology; D008969:Molecular Sequence Data; D009994:Osmolar Concentration; D010447:Peptide Hydrolases / Q000378:metabolism; D020675:Peroxisomes / Q000378:metabolism / Q000502:physiology*; D017434:Protein Structure, Tertiary
chemical_list: D005227:Fatty Acids; D010447:Peptide Hydrolases; D034281:Dynamins