title: Genetic structure of Streptococcus pneumoniae isolated from children in a tertiary care university hospital, in Korea, 1995 to 2005.
abstract: We investigated the genetic structure of Streptococcus pneumoniae isolated from Korean children prior to the use of heptavalent pneumococcal conjugate vaccine (PCV7). A total of 278 pneumococcal isolates were obtained from invasive (n = 77) and noninvasive (n = 201) specimens from children < 5 years of age that visited the Seoul National University Children's Hospital between 1995 and 2005; these samples were serotyped. Two hundred seventy-eight isolates were characterized by multilocus sequence typing, antibiotic susceptibility pattern testing with the E-test. The common serotypes were 19F (22.7%), 23F (19.1%), 19A (14.4%), 6B (11.2%), 6A (8.3%), 14 (5.4%), and 9V (4.3%); these 7 serotypes accounted for 85.3% of the 278 isolates. The isolates belonged to 23 serotypes and 81 sequence types (STs) (including 48 newly discovered STs). The eBURST analysis showed 7 clonal complexes (CCs). CC271 was the main CC of serogroup 19; CC81 and CC3115, serotype 6A; CC81 and CC880, serotype 23F; CC90, serotype 6B; CC166, serotype 9V; and CC554, serotype 14. Nonsusceptibility rates to penicillin and erythromycin among the invasive isolates were 9.1% and 94.8%, respectively. The resultant database may be useful for postvaccine surveillance.
authors.affiliation: Department of Pediatrics, Seoul National University College of Medicine, Seoul 110-799, South Korea.
authors.forename: Byung Wook
authors.initials: BW
authors.lastname: Eun
authors.forename: Sun Jung
authors.initials: SJ
authors.lastname: Kim
authors.forename: Eun Young
authors.initials: EY
authors.lastname: Cho
authors.forename: Jina
authors.initials: J
authors.lastname: Lee
authors.forename: Eun Hwa
authors.initials: EH
authors.lastname: Choi
authors.forename: Hoan Jong
authors.initials: HJ
authors.lastname: Lee
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002675:Child, Preschool; D024881:Drug Resistance, Bacterial; D000069443:Heptavalent Pneumococcal Conjugate Vaccine; D006785:Hospitals, University / Q000706:statistics & numerical data*; D006801:Humans; D008826:Microbial Sensitivity Tests; D058885:Multilocus Sequence Typing / Q000379:methods; D011008:Pneumococcal Infections / Q000453:epidemiology* / Q000382:microbiology / Q000517:prevention & control; D022242:Pneumococcal Vaccines; D056910:Republic of Korea / Q000453:epidemiology; D012703:Serotyping; D013296:Streptococcus pneumoniae / Q000145:classification* / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D000900:Anti-Bacterial Agents; D000069443:Heptavalent Pneumococcal Conjugate Vaccine; D022242:Pneumococcal Vaccines