title: [Effects of RNA interference targeting four different genes on the growth and proliferation of nasopharyngeal carcinoma CNE-2Z cells].
abstract: OBJECTIVE
  To explore the effects of RNA interference (RNAi) targeting four different genes (VEGF, c-myc, survivin, hTERT) on the growth and proliferation of nasopharyngeal carcinoma (NPC) CNE-2Z cells.
  
  
  METHODS
  Plasmid-1 targeted all four genes, plasmid-2, 3, 4 and 5 targeted VEGF, c-myc, survivin and hTERT respectively. These plasmids were transfected separately into human NPC CNE-2Z cells and xenograft tumors in nude mice. The expressions of plasmids in NPC CNE-2Z cells and xenograft tumors were observed. Cell proliferation was detected with MTT assay. The inhibitory effects on target genes were evaluated with RT-PCR and Western blot respectively. The effects of the plasmids on the biological behavior of CNE-2Z cells were observed with Transwell invasion chamber model. Apoptosis was determined with flow cytometer. The inhibitory effect of the plasmids on xenograft tumors were observed in nude mice.
  
  
  RESULTS
  CNE-2Z cell proliferation was significantly inhibited and in vitro invasion ability was significantly decreased in the plasmid-1 group compared with those in the plasmid 2 - 5 groups (all P < 0.05). mRNA and protein expressions of all four genes decreased in the plasmid-1 group. The apoptosis rate in the plasmid-1 group was higher than that in the plasmid 2 - 5 groups (all P < 0.05). Growth of xenograft tumors in nude mice were significantly inhibited in the plasmid 1 - 5 groups, particularly in the plasmid-1 group.
  
  
  CONCLUSIONS
  RNA interference targeting multiple genes can effectively inhibit NPC proliferation and induce apoptosis, which provides an experiment basis for NPC gene therapy.
authors.affiliation: Department of Otorhinolaryngology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Song
authors.forename: Ming-min
authors.initials: MM
authors.lastname: Dong
authors.forename: Hai-feng
authors.initials: HF
authors.lastname: Yang
mesh_terms: D000818:Animals; D017209:Apoptosis; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D018390:Gene Targeting; D016259:Genes, myc; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000235:genetics; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009303:Nasopharyngeal Neoplasms / Q000235:genetics* / Q000473:pathology; D010957:Plasmids; D034622:RNA Interference; D000077022:Survivin; D019098:Telomerase / Q000235:genetics; D014162:Transfection; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics
chemical_list: C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D000077022:Survivin; D042461:Vascular Endothelial Growth Factor A; D019098:Telomerase