title: Two-step silylation procedure for the unified analysis of 190 doping control substances in human urine samples by GC-MS.
abstract: BACKGROUND
  While a number of different derivatization procedures for screening GC-MS analysis of prohibited substances are followed by doping control laboratories, a unified derivatization procedure for the GC-MS analysis of 190 different doping agents was developed.
  
  
  RESULTS
  Following preliminary experiments, a two-step derivatization procedure was selected. The evaluation of various silylation parameters, such as reagent composition, reaction time, reaction temperature, catalysts and microwave oven reaction time, for this procedure was carried out.
  
  
  CONCLUSIONS
  The suitability of the developed procedure was demonstrated through application on urine samples at concentration levels of the minimum required performance limit for all tested substances. This new derivatization procedure, which significantly decreases time and cost, is suitable for a routine basis application.
authors.affiliation: Doping Control Laboratory of Athens, Olympic Athletic Center of Athens, 15123 Maroussi, Greece.
authors.forename: P
authors.initials: P
authors.lastname: Kiousi
authors.forename: Y S
authors.initials: YS
authors.lastname: Angelis
authors.forename: E
authors.initials: E
authors.lastname: Lyris
authors.forename: M
authors.initials: M
authors.lastname: Koupparis
authors.forename: A C
authors.initials: AC
authors.lastname: Calokerinos
authors.forename: J
authors.initials: J
authors.lastname: Atta-Politou
authors.forename: C G
authors.initials: CG
authors.lastname: Georgakopoulos
mesh_terms: D004300:Doping in Sports / Q000191:economics / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D012821:Silanes / Q000737:chemistry*; D015813:Substance Abuse Detection / Q000191:economics / Q000379:methods*; D013997:Time Factors; D016482:Urinalysis / Q000191:economics / Q000379:methods*
chemical_list: D012821:Silanes