title: Ttd1a promoter is involved in DNA-protein binding by salt and light stresses.
abstract: Stress modulation of retrotransposons may play a role in generating host genetic plasticity in response to environmental stress. Transposable elements have been suggested to contribute to the evolution of genes, by providing cis-regulatory elements leading to changes in expression patterns. Indeed, their promoter elements are similar to those of plant defence genes and may bind similar defence-induced transcription factors. We previously isolated a new Ty1-copia retrontrasposon named Ttd1a and showed its activation and mobilization in salt and light stresses. Here, using a retard mobility assay in Triticum durum L. crude extracts, we showed that the CAAT motif present in the Ttd1a retrotransposon promoter, is involved in DNA-protein binding under salt and light stresses and therefore in the regulation of Ttd1a activity. Data presented in this paper suggest that nuclear proteins can interact with the CAAT motif either directly or indirectly and enhance Ttd1a by a specific ligand-dependent activation under stress.
authors.affiliation: Department of Life Science, II University of Naples, via Vivaldi 43, 81100, Caserta, Italy.
authors.forename: Pasqualina
authors.initials: P
authors.lastname: Woodrow
authors.forename: Giovanni
authors.initials: G
authors.lastname: Pontecorvo
authors.forename: Loredana F
authors.initials: LF
authors.lastname: Ciarmiello
authors.forename: Amodio
authors.initials: A
authors.lastname: Fuggi
authors.forename: Petronia
authors.initials: P
authors.lastname: Carillo
mesh_terms: D001483:Base Sequence; D019295:Computational Biology; D018744:DNA, Plant / Q000378:metabolism*; D024202:Electrophoretic Mobility Shift Assay; D008027:Light; D009154:Mutation / Q000235:genetics; D010940:Plant Proteins / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D011485:Protein Binding / Q000187:drug effects / Q000528:radiation effects; D018626:Retroelements / Q000235:genetics*; D012965:Sodium Chloride / Q000494:pharmacology*; D013312:Stress, Physiological / Q000187:drug effects / Q000235:genetics / Q000528:radiation effects; D014157:Transcription Factors / Q000378:metabolism; D014908:Triticum / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects
chemical_list: D018744:DNA, Plant; D010940:Plant Proteins; D018626:Retroelements; D014157:Transcription Factors; D012965:Sodium Chloride