title: [Investigation of the interaction of repair DNA polymerase beta and autonomous 3' --> 5'-exonucleases TREX1 and TREX2].
abstract: The possibility of interaction of recombinant proteins of human repair DNA polymerase beta with proofreading 3' --> 5'-exonucleases TREX1 and TREX2 was investigated in vitro for the first time. The results of gel filtration analysis show the formation of a complex between 3' --> 5' -exonucleases mTREX1 and hTREX2 and DNA polymerase beta. DNA polymerase activity is shown to increase four-fold in the presence of 3' --> 5'-exonuclease TREX2. The experiments with the use of immunodot and Western blot assays on the binding of DNA-polymerase beta with 3' --> 5'-exonucleases TREX1 and TREX2 immobilized on a nitrocellulose membrane provided additional evidence on the direct association of the above proteins in complexes.
authors.forename: N V
authors.initials: NV
authors.lastname: Beliakova
authors.forename: O K
authors.initials: OK
authors.lastname: Legina
authors.forename: N L
authors.initials: NL
authors.lastname: Ronzhina
authors.forename: I V
authors.initials: IV
authors.lastname: Shevelev
authors.forename: V M
authors.initials: VM
authors.lastname: Krutiakov
mesh_terms: D000818:Animals; D019951:DNA Polymerase beta / Q000737:chemistry* / Q000235:genetics; D004260:DNA Repair; D045643:DNA Repair Enzymes / Q000737:chemistry* / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli / Q000235:genetics; D005090:Exodeoxyribonucleases / Q000737:chemistry* / Q000235:genetics; D006801:Humans; D015151:Immunoblotting; D051379:Mice; D010750:Phosphoproteins / Q000737:chemistry* / Q000235:genetics; D011485:Protein Binding; D025941:Protein Interaction Mapping; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D010750:Phosphoproteins; D011994:Recombinant Proteins; D019951:DNA Polymerase beta; D005090:Exodeoxyribonucleases; C486104:TREX2 protein, human; C429526:three prime repair exonuclease 1; D045643:DNA Repair Enzymes