title: "Click-made" biaryl-linker improving efficiency in protein labelling for the membrane target protein of a bioactive compound.
abstract: We report on the design, synthesis and assessment of a novel biaryl-linked (BArL) molecular probe for the exploration of low-abundant target proteins for bioactive compounds based on the activity based protein profiling (ABPP) approach. Surprisingly, the performance of the BArL probe was better than that of the stepwise tagging approach that is considered to be the most effective method used in ABPP study.
authors.affiliation: Department of Chemistry, Tohoku University, 6-3 Aramaki-aza Aoba, Aoba-ku, Sendai 980-8578, Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Nakamura
authors.forename: Sho
authors.initials: S
authors.lastname: Inomata
authors.forename: Makoto
authors.initials: M
authors.lastname: Ebine
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Manabe
authors.forename: Izumi
authors.initials: I
authors.lastname: Iwakura
authors.forename: Minoru
authors.initials: M
authors.lastname: Ueda
mesh_terms: D008565:Membrane Proteins / Q000737:chemistry*; D008958:Models, Molecular; D015394:Molecular Structure
chemical_list: D008565:Membrane Proteins