title: Identification and characterization of a novel zebrafish semaphorin.
abstract: The semaphorin gene family contains numerous secreted and transmembrane proteins. Some of them function as the repulsive and attractive axon guidance molecules during development. Herein, we report the cloning and characterization of a novel member of zebrafish semaphorin gene, semaphorin 6E (sema6E). Sema6E is expressed predominantly in the nervous system during embryogenesis. Results also show that Sema6E binds Plexin-A1, but not other Plexins. Sema6E chemorepels not only dorsal root ganglion axons but also sympathetic axons. Therefore, Sema6E might utilize Plexin-A1 as a receptor to repel axons of the specific types during development.
authors.affiliation: Department of Biochemistry, Cancer Research Institute, Sapporo Medical University School of Medicine, S-1 W-17, Chuo-ku, Sapporo, Hokkaido 060-8556, Japan. taniguti@sapmed.ac.jp
authors.forename: Masahiko
authors.initials: M
authors.lastname: Taniguchi
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Masuda
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Mikami
authors.forename: Masafumi
authors.initials: M
authors.lastname: Kimura
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Yoshida
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Mishina
authors.forename: Takao
authors.initials: T
authors.lastname: Shimizu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D047108:Embryonic Development / Q000502:physiology; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D055495:Neurogenesis / Q000502:physiology; D016133:Polymerase Chain Reaction; D039961:Semaphorins / Q000235:genetics* / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D015027:Zebrafish / Q000502:physiology*; D029961:Zebrafish Proteins / Q000235:genetics* / Q000378:metabolism*
chemical_list: D039961:Semaphorins; D029961:Zebrafish Proteins