title: Mass analysis of biological macromolecules at atmospheric pressure using nonresonant femtosecond laser vaporization and electrospray ionization.
abstract: A nonresonant femtosecond laser pulse, with an intensity of 10(13) Wcm(-2), vaporizes proteins and biomolecules intact, regardless of molecular structure, size or electronic structure for subsequent electrospray ionization and transfer into a mass spectrometer. Rapid, direct analysis from dried sample, aqueous solution and cellular material is demonstrated at atmospheric pressure using laser electrospray mass spectrometry (LEMS). Measurements are presented for lysozyme (14.3 kDa), hemoglobin from human blood, ovalbumin (45 kDa) from hen egg white and phospholipids from hen egg yolk. Mass analysis of biological material is performed without dilution, extraction or sample preparation, other than placing the biological material onto the sample plate.
authors.affiliation: Temple University Department of Chemistry, 1901 N 13th Street, Philadelphia, Pennsylvania 19122, United States.
authors.forename: Elizabeth J
authors.initials: EJ
authors.lastname: Judge
authors.forename: John J
authors.initials: JJ
authors.lastname: Brady
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Levis
mesh_terms: D000818:Animals; D002645:Chickens; D006454:Hemoglobins; D006801:Humans; D007834:Lasers; D046911:Macromolecular Substances / Q000032:analysis*; D009113:Muramidase; D010047:Ovalbumin; D010743:Phospholipids; D011506:Proteins; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D006454:Hemoglobins; D046911:Macromolecular Substances; D010743:Phospholipids; D011506:Proteins; D010047:Ovalbumin; D009113:Muramidase