title: Genome annotation and intraviral interactome for the Streptococcus pneumoniae virulent phage Dp-1.
abstract: Streptococcus pneumoniae causes several diseases, including pneumonia, septicemia, and meningitis. Phage Dp-1 is one of the very few isolated virulent S. pneumoniae bacteriophages, but only a partial characterization is currently available. Here, we confirmed that Dp-1 belongs to the family Siphoviridae. Then, we determined its complete genomic sequence of 56,506 bp. It encodes 72 open reading frames, of which 44 have been assigned a function. We have identified putative promoters, Rho-independent terminators, and several genomic clusters. We provide evidence that Dp-1 may be using a novel DNA replication system as well as redirecting host protein synthesis through queuosine-containing tRNAs. Liquid chromatography-mass spectrometry analysis of purified phage Dp-1 particles identified at least eight structural proteins. Finally, using comprehensive yeast two-hybrid screens, we identified 156 phage protein interactions, and this intraviral interactome was used to propose a structural model of Dp-1.
authors.affiliation: Département de Biochimie, de Microbiologie et Bio-Informatiques, Faculté des Sciences et de Génie, Groupe de Recherche en Ecologie Buccale, Faculté de Médecine Dentaire, Félix d' Hérelle Reference Center for Bacterial Viruses, Université Laval, Québec, Canada.
authors.forename: Mourad
authors.initials: M
authors.lastname: Sabri
authors.forename: Roman
authors.initials: R
authors.lastname: Häuser
authors.forename: Marc
authors.initials: M
authors.lastname: Ouellette
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.forename: Mohammed
authors.initials: M
authors.lastname: Dehbi
authors.forename: Greg
authors.initials: G
authors.lastname: Moeck
authors.forename: Ernesto
authors.initials: E
authors.lastname: García
authors.forename: Björn
authors.initials: B
authors.lastname: Titz
authors.forename: Peter
authors.initials: P
authors.lastname: Uetz
authors.forename: Sylvain
authors.initials: S
authors.lastname: Moineau
mesh_terms: D002853:Chromatography, Liquid; D004261:DNA Replication; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D023061:Gene Order; D005814:Genes, Viral; D016679:Genome, Viral; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D005810:Multigene Family; D016366:Open Reading Frames; D011401:Promoter Regions, Genetic; D014176:Protein Biosynthesis; D017422:Sequence Analysis, DNA; D017906:Siphoviridae / Q000145:classification / Q000648:ultrastructure; D017108:Streptococcus Phages / Q000145:classification / Q000235:genetics* / Q000648:ultrastructure; D013296:Streptococcus pneumoniae / Q000821:virology*; D013728:Terminator Regions, Genetic; D015678:Viral Structural Proteins / Q000032:analysis
chemical_list: D004279:DNA, Viral; D015678:Viral Structural Proteins