title: Elimination of LC-MS/MS matrix effect due to phospholipids using specific solid-phase extraction elution conditions.
abstract: BACKGROUND
  The objective is to find elution conditions using different solid-phase extraction chemistries to reduce the amount of phospholipids in plasma extract, which is associated with matrix effect in LC-MS.
  
  
  RESULTS
  Phospholipids' recovery was reduced by decreasing the eluents' methanol content applied to silica-based and polymer-based reversed-phase solid-phase extraction cartridges while 100% acetonitrile applied to the silica-based cartridges drastically minimized the phospholipids' elution. Silanol interactions are involved in the increased retention of phospholipids with silica-based reversed-phase cartridges when using high percentages of ACN in eluents. The bioanalytical usefulness of these findings was confirmed by successful extraction recovery of pharmaceutical compounds.
  
  
  CONCLUSIONS
  Combinations of specific eluents and reversed-phase solid-phase extraction cartridges were found to prevent matrix effect due to phospholipids.
authors.affiliation: Algorithme Pharma Inc., Laval (Montreal), Quebec, Canada.
authors.forename: Mathieu
authors.initials: M
authors.lastname: Lahaie
authors.forename: Jean-Nicholas
authors.initials: JN
authors.lastname: Mess
authors.forename: Milton
authors.initials: M
authors.lastname: Furtado
authors.forename: Fabio
authors.initials: F
authors.lastname: Garofolo
mesh_terms: D000097:Acetonitriles / Q000737:chemistry; D016477:Artifacts; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D008244:Lysophosphatidylcholines / Q000737:chemistry; D000432:Methanol / Q000737:chemistry; D010743:Phospholipids / Q000097:blood / Q000737:chemistry* / Q000302:isolation & purification*; D011108:Polymers / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry; D052616:Solid Phase Extraction / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014867:Water / Q000737:chemistry
chemical_list: D000097:Acetonitriles; D008244:Lysophosphatidylcholines; D010743:Phospholipids; D011108:Polymers; D014867:Water; D012822:Silicon Dioxide; D000432:Methanol; C032159:acetonitrile