title: Molecular cloning and expression analysis of a F-type lectin gene from Japanese sea perch (Lateolabrax japonicus).
abstract: The techniques of homology cloning and anchored PCR were used to clone the fucose-binding lectin (F-type lectin) gene from Japanese sea perch (Lateolabrax Japonicus). The full-length cDNA of sea perch F-lectin (JspFL) contained a 5' untranslated region (UTR) of 39 bp, an ORF of 933 bp encoding a polypeptide of 310 amino acids with an estimated molecular mass of 10.82 kDa and a 3' UTR of 332 bp. The searches for nucleotides and protein sequence similarities with BLAST analysis indicated that the deduced amino acid sequence of JspFL was homological to the Fucose-binding lectin in other fish species. In the JspFL deduced amino acid sequence, two tandem domains that exhibit the eel carbohydrate-recognition sequence motif were found. The temporal expressions of gene in the different tissues were measured by real-time PCR. And the mRNA expressions of the gene were constitutively expressed in tissues including spleen, head-kidney, liver, gill, and heart. The JspFL expression in spleen was different during the stimulated time point, 2 h later the expression level became up-regulated, and 6 h later the expression level became down-regulated. The result indicated that JspFL was constitutive and inducible expressed and could play a critical role in the host-pathogen interaction.
authors.affiliation: Biotechnology and Aquiculture Laboratory, The South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 231 Xingangxi Road, Guangzhou, 510300, People's Republic of China. qiu902@126.com
authors.forename: Lihua
authors.initials: L
authors.lastname: Qiu
authors.forename: Liansheng
authors.initials: L
authors.lastname: Lin
authors.forename: Keng
authors.initials: K
authors.lastname: Yang
authors.forename: Hanhua
authors.initials: H
authors.lastname: Zhang
authors.forename: Jianzhu
authors.initials: J
authors.lastname: Li
authors.forename: Falin
authors.initials: F
authors.lastname: Zou
authors.forename: Shigui
authors.initials: S
authors.lastname: Jiang
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects; D007564:Japan; D037102:Lectins / Q000235:genetics* / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D008969:Molecular Sequence Data; D010471:Perches / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013154:Spleen / Q000187:drug effects / Q000378:metabolism; D013997:Time Factors
chemical_list: D037102:Lectins; D008070:Lipopolysaccharides; D012333:RNA, Messenger; C027112:fucose-binding lectin