title: Temporal and functional analysis of DNA replicated in early S phase.
abstract: In summary, recently developed technologies have begun to draw back the curtain of mystery that obscures some of the basic mechanisms of DNA replication at multiple levels. Studies using extended DNA and chromatin fiber techniques have proven valuable for identifying the location of origins of replication at specific genomic sites and determining their temporal order of replication, for identifying and quantifying sites of DNA damage and localizing chromatin proteins in relation to sites of DNA replication. The future potential of these methods include further discoveries in functional genomics and contributions to the elucidation of the histone code. Such studies could prove very valuable in studies of the mechanisms of cancer development, aging, and other processes of disordered genomic functioning.
authors.affiliation: Department of Pathology and Laboratory Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA. uncdgk@med.unc.edu
authors.forename: David G
authors.initials: DG
authors.lastname: Kaufman
authors.forename: Stephanie M
authors.initials: SM
authors.lastname: Cohen
authors.forename: Paul D
authors.initials: PD
authors.lastname: Chastain
mesh_terms: D002843:Chromatin / Q000378:metabolism / Q000648:ultrastructure; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D004261:DNA Replication; D006801:Humans; D009369:Neoplasms / Q000235:genetics / Q000378:metabolism; D016196:S Phase
chemical_list: D002843:Chromatin; D004247:DNA