title: Analysis of six benzodiazepines in vitreous humor by high-performance liquid chromatography-photodiode-array detection.
abstract: The purpose of this study was to validate a high-performance liquid chromatography-photodiode-array detetion method for the determination of six benzodiazepines in vitreous humor. The sample preparation was carried out using solid-phase extraction with Oasis HLB cartridges and 10% acetic acid/MeOH as elution solvent. The vitreous humor is less affected by postmortem changes and is a very useful sample when blood or urine specimens are not available. Linear curves for bromazepam, alprazolam, lorazepam, lormetazepam, diazepam, and tetrazepam were obtained within the range 0.03-3 μg/mL, with coefficients of correlation lower than 0.999. The limit of detection was 3 ng/mL, and the lower limit of quantification was 30 ng/mL for each benzodiazepine. Intra- and interassay for precision and accuracy provided results less than 16.81% and 16.78%, respectively. Recoveries were higher than 68.51% in all cases. Finally, the method was applied to determine benzodiazepines in vitreous humor from intoxicated patients.
authors.affiliation: Institute of Legal Medicine, Forensic Toxicology Service, Faculty of Medicine, C/ San Francisco s/n, 15782 Santiago de Compostela, Spain.
authors.forename: P
authors.initials: P
authors.lastname: Cabarcos
authors.forename: M J
authors.initials: MJ
authors.lastname: Tabernero
authors.forename: I
authors.initials: I
authors.lastname: Álvarez
authors.forename: P
authors.initials: P
authors.lastname: López
authors.forename: P
authors.initials: P
authors.lastname: Fernández
authors.forename: A M
authors.initials: AM
authors.lastname: Bermejo
mesh_terms: D001569:Benzodiazepines / Q000032:analysis*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D053593:Forensic Toxicology / Q000379:methods; D006801:Humans; D006993:Hypnotics and Sedatives / Q000032:analysis*; D057230:Limit of Detection; D015203:Reproducibility of Results; D052616:Solid Phase Extraction; D015813:Substance Abuse Detection; D014822:Vitreous Body / Q000737:chemistry*
chemical_list: D006993:Hypnotics and Sedatives; D001569:Benzodiazepines