title: A juvenile murine heart failure model of pressure overload.
abstract: Persistent pressure overload can cause cardiac hypertrophy and progressive heart failure (HF). The authors developed a pressure-overload HF model of juvenile mice to study the cardiac response to pressure overload that may be applicable to clinical processes in children. Severe thoracic aortic banding (sTAB) was performed using a 28-gauge needle for 40 juvenile (age, 3 weeks) and 47 adult (age, 6 weeks) C57BL/6 male mice. To monitor the structural and functional changes, M-mode echocardiography was performed for conscious mice that had undergone sTAB and sham operation. Cardiac hypertrophy, dilation, and HF occurred in both juvenile and adult mice after sTAB. Compared with adults, juvenile HF is characterized by greater impairment of ventricular contractility and less hypertrophy. In addition, juvenile mice had significantly higher rates of survival than adult mice during the early postoperative weeks. Consistent with clinical HF seen in children, juvenile banded mice demonstrated a lower growth rate than either adult banded mice or juvenile control mice that had sham operations. The authors first developed a juvenile murine model of pressure-overload HF. Learning the unique characteristics of pressure-overload HF in juveniles should aid in understanding age-specific pathologic changes for HF development in children.
authors.affiliation: Department of Pediatrics, Children's Healthcare of Atlanta and Sibley Heart Center, Emory School of Medicine, Atlanta, GA 30322, USA.
authors.forename: Kristopher M
authors.initials: KM
authors.lastname: Cumbermack
authors.forename: Jun
authors.initials: J
authors.lastname: Cheng
authors.forename: Yibing
authors.initials: Y
authors.lastname: Nong
authors.forename: William T
authors.initials: WT
authors.lastname: Mahle
authors.forename: Ronald W
authors.initials: RW
authors.lastname: Joyner
authors.forename: William L
authors.initials: WL
authors.lastname: Border
authors.forename: Mary B
authors.initials: MB
authors.lastname: Wagner
authors.forename: Derek A
authors.initials: DA
authors.lastname: Fyfe
authors.forename: Traci
authors.initials: T
authors.lastname: Leong
authors.forename: Yanggan
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000367:Age Factors; D000818:Animals; D001783:Blood Flow Velocity; D001794:Blood Pressure; D006332:Cardiomegaly / Q000000981:diagnostic imaging / Q000473:pathology*; D004195:Disease Models, Animal; D018450:Disease Progression; D004452:Echocardiography; D006333:Heart Failure / Q000000981:diagnostic imaging / Q000473:pathology*; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D051379:Mice; D009200:Myocardial Contraction; D009206:Myocardium; D013908:Thoracotomy; D014481:United States