title: [Downregulation of activated leukemic oncogenes AML1-ETO and RUNX1(K83N) expression with RNA-interference].
abstract: In the present study we have applied the siRNA approach for substantial reduction of AML1-ETO and RUNX1 (K83N) expression, which are frequently found in the leukemic cells. We have designed small hairpin RNAs (shRNA) for targeting AML1-ETO oncogene and a region close to the 5'-untranslated region of mRNA for the mutant RUNX1 (K83N) oncogene and expressed the shRNAs in lentiviral vectors. We report a stable reduction in expression of the oncogenes following the introduction of shRNAs into cells.
authors.forename: P V
authors.initials: PV
authors.lastname: Spirin
authors.forename: F
authors.initials: F
authors.lastname: Baskaran
authors.forename: N N
authors.initials: NN
authors.lastname: Orlova
authors.forename: A V
authors.initials: AV
authors.lastname: Rulina
authors.forename: N A
authors.initials: NA
authors.lastname: Nikitenko
authors.forename: E L
authors.initials: EL
authors.lastname: Chernolovskaia
authors.forename: M A
authors.initials: MA
authors.lastname: Zenkova
authors.forename: V V
authors.initials: VV
authors.lastname: Vlasov
authors.forename: P M
authors.initials: PM
authors.lastname: Rubtsov
authors.forename: P M
authors.initials: PM
authors.lastname: Chumakov
authors.forename: C
authors.initials: C
authors.lastname: Stocking
authors.forename: V S
authors.initials: VS
authors.lastname: Prasolov
mesh_terms: D020121:5' Untranslated Regions / Q000235:genetics; D019943:Amino Acid Substitution; D000818:Animals; D045744:Cell Line, Tumor; D050676:Core Binding Factor Alpha 2 Subunit / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000235:genetics; D015536:Down-Regulation; D015973:Gene Expression Regulation, Leukemic; D057809:HEK293 Cells; D006801:Humans; D007938:Leukemia / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D020125:Mutation, Missense; D015514:Oncogene Proteins, Fusion / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000235:genetics; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D000075142:RUNX1 Translocation Partner 1 Protein
chemical_list: D020121:5' Untranslated Regions; C107844:AML1-ETO fusion protein, human; D050676:Core Binding Factor Alpha 2 Subunit; D015514:Oncogene Proteins, Fusion; D034741:RNA, Small Interfering; D000075142:RUNX1 Translocation Partner 1 Protein; C493728:RUNX1 protein, human