title: Effectiveness of scaffolds with pre-seeded mesenchymal stem cells in bone regeneration--assessment of osteogenic ability of scaffolds implanted under the periosteum of the cranial bone of rats.
abstract: To date, there has been no study on the development of novel regimens based on the following tissue engineering principles: seeding and culturing mesenchymal stem cells (MSCs) on a scaffold before surgery or injecting cultured MSCs into a scaffold during surgery. The purpose of this study was to assess the in vivo osteogenic ability of scaffold/MSCs implanted beneath the periosteum of the cranial bone of rats in three different sample groups: one in which MSCs were pre-seeded and cultured on a scaffold to produce the 3-D woven fabric scaffold/MSC composite using osteo-lineage induction medium, one in which cultured MSCs produced by osteo-lineage induction in cell cultivation flasks were injected into a scaffold during surgery and a control group, in which only the 3-D woven fabric scaffold was implanted. The results indicate that pre-seeding MSCs on a scaffold leads to a higher osteogenic ability than injecting cultured MSCs into a scaffold during surgery.
authors.affiliation: Department of Regenerative Medicine, Foundation for Biomedical Research and Innovation, Kobe, Japan.
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Baba
authors.forename: Takeomi
authors.initials: T
authors.lastname: Inoue
authors.forename: Yoshiya
authors.initials: Y
authors.lastname: Hashimoto
authors.forename: Daisuke
authors.initials: D
authors.lastname: Kimura
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Ueda
authors.forename: Kana
authors.initials: K
authors.lastname: Sakai
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Matsumoto
authors.forename: Chiaki
authors.initials: C
authors.lastname: Hiwa
authors.forename: Taiji
authors.initials: T
authors.lastname: Adachi
authors.forename: Masaki
authors.initials: M
authors.lastname: Hojo
mesh_terms: D020341:Absorbable Implants; D000818:Animals; D001854:Bone Marrow Cells / Q000502:physiology; D001861:Bone Regeneration / Q000502:physiology*; D002452:Cell Count; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology; D019070:Cell Lineage; D049109:Cell Proliferation; D048430:Cell Shape; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000502:physiology; D010006:Osteoblasts / Q000502:physiology; D010012:Osteogenesis / Q000502:physiology*; D010521:Periosteum / Q000601:surgery*; D053657:Platelet-Rich Plasma / Q000502:physiology; D011091:Polyesters; D011108:Polymers / Q000737:chemistry; D011474:Prosthesis Design; D051381:Rats; D011916:Rats, Inbred F344; D011923:Rats, Nude; D012886:Skull / Q000601:surgery*; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide)