title: The binding of CNA35 contrast agents to collagen fibrils.
abstract: CryoTEM demonstrates that a CNA35-bearing liposomal MRI contrast agent selectively binds to poorly assembled collagen type I as opposed to well-assembled collagen fibrils, whereas monomeric CNA35 binds to all forms of collagen. It is shown that upon conjugation to liposomes and micelles CNA35 loses its ability to dissociate ordered collagen fibrils and thereby to create its own binding sites.
authors.affiliation: Biomedical NMR, Department of Biomedical Engineering, Eindhoven University of Technology, P.O. Box 513, 5600 MB Eindhoven, The Netherlands.
authors.forename: Honorius M H F
authors.initials: HM
authors.lastname: Sanders
authors.forename: Michele
authors.initials: M
authors.lastname: Iafisco
authors.forename: Emilie M
authors.initials: EM
authors.lastname: Pouget
authors.forename: Paul H H
authors.initials: PH
authors.lastname: Bomans
authors.forename: Fabio
authors.initials: F
authors.lastname: Nudelman
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Falini
authors.forename: Gijsbertus
authors.initials: G
authors.lastname: de With
authors.forename: Maarten
authors.initials: M
authors.lastname: Merkx
authors.forename: Gustav J
authors.initials: GJ
authors.lastname: Strijkers
authors.forename: Klaas
authors.initials: K
authors.lastname: Nicolay
authors.forename: Nico A J M
authors.initials: NA
authors.lastname: Sommerdijk
mesh_terms: D000818:Animals; D001665:Binding Sites; D003094:Collagen / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D003287:Contrast Media / Q000737:chemistry / Q000378:metabolism*; D020285:Cryoelectron Microscopy; D006736:Horses; D008081:Liposomes / Q000737:chemistry*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008823:Micelles; D046529:Microscopy, Electron, Transmission
chemical_list: D003287:Contrast Media; D008081:Liposomes; D008823:Micelles; D003094:Collagen