title: Size-exclusion separation of proteins using a biocompatible polymeric monolithic capillary column with mesoporosity.
abstract: Biocompatible poly(ethylene glycol methyl ether acrylate-co-polyethylene glycol diacrylate) monoliths were prepared for size exclusion chromatography (SEC) of proteins in the capillary format using Brij 58P in a mixture of hexanes and dodecanol as porogens. The monolithic columns provided size separation of four proteins in 20 mM sodium phosphate buffer (pH 7.0) containing 0.15 M NaCl, and there was a linear relationship between the retention times and the logarithmic values of the molecular weights. Compared to SEC monoliths previously synthesized using a triblock copolymer of polyethylene oxide and polypropylene oxide, an increase in mesoporosity was confirmed by inverse size exclusion chromatography. As a result, improved protein separation in the high molecular weight range and reduced column back-pressure were observed.
authors.affiliation: Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT 84602, USA.
authors.forename: Yun
authors.initials: Y
authors.lastname: Li
authors.forename: H Dennis
authors.initials: HD
authors.lastname: Tolley
authors.forename: Milton L
authors.initials: ML
authors.lastname: Lee
mesh_terms: D001704:Biopolymers / Q000737:chemistry*; D002850:Chromatography, Gel / Q000295:instrumentation* / Q000379:methods; D008970:Molecular Weight; D011092:Polyethylene Glycols / Q000737:chemistry*; D016062:Porosity; D011506:Proteins / Q000032:analysis / Q000302:isolation & purification*
chemical_list: D001704:Biopolymers; D011506:Proteins; C437167:poly(ethylene glycol)diacrylate; D011092:Polyethylene Glycols