title: Analysis of phagosomal proteomes: from latex-bead to bacterial phagosomes.
abstract: Phagosomal proteome characterization has contributed significantly to the understanding of host-pathogen interaction and the mechanism of infectious diseases caused by intracellular bacteria. The latex bead-containing phagosome has been widely used as a model system to study phagosomal proteomes at a global level. In contrast, the study of bacteria-containing phagosomes at a similar level has just begun. A number of intracellular microbial species are studied for their proteomes during the invasion of a host, providing insight into their metabolic adaptation in host cells and interaction with host-cell antimicrobial environments. In this review, we attempt to summarize the most recent advancements in the proteomic study of microbial phagosomes, especially those originating from mouse or human cells. We also briefly describe the proteomics of latex bead-containing phagosomes because they are often used as model phagosomes for study. We provide descriptions on major biological and technological components in phagosomal proteome studies. We also discuss the role of phagosomal proteome study in the broader horizon of systems biology and the technological challenges in phagosomal proteome characterization.
authors.affiliation: Center for Pharmaceutical Biotechnology, University of Illinois at Chicago, Chicago, IL 60607, USA. qkli@uic.edu
authors.forename: Qingbo
authors.initials: Q
authors.lastname: Li
authors.forename: Chinnaswamy
authors.initials: C
authors.lastname: Jagannath
authors.forename: Prahlad K
authors.initials: PK
authors.lastname: Rao
authors.forename: Christopher R
authors.initials: CR
authors.lastname: Singh
authors.forename: Giovanni
authors.initials: G
authors.lastname: Lostumbo
mesh_terms: D000818:Animals; D015415:Biomarkers; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008863:Microspheres; D010587:Phagocytosis / Q000502:physiology*; D010588:Phagosomes / Q000737:chemistry* / Q000378:metabolism; D040901:Proteomics; D051381:Rats; D049490:Systems Biology; D020691:rab GTP-Binding Proteins
chemical_list: D015415:Biomarkers; D020691:rab GTP-Binding Proteins