title: Degree of immobilization of synthetic RGDS(PO(3)H(2))PA peptides on titanium surfaces.
abstract: The purpose of this study was to characterize the chemical interaction between titanium surfaces and the peptide RGDS(PO(3)H(2))PA (P-RGD) synthesized from RGD peptide (RGD) and o-phospho-L-serine (P-Ser), and to determine the degree of peptide immobilization on the titanium surface. X-ray photoelectron spectroscopy showed that the adsorption amount of RGD was significantly smaller than those of P-Ser and P-RGD (p<0.05). Furthermore, although it appeared that P-RGD bonded to the surface, ultrasonic rinsing with water caused it to dissociate, releasing RGD and leaving only S(PO(3)H(2))PA bonded to the surface. These findings show that although it remains difficult to obtain a stable P-RGD layer, the phosphate functional group greatly improves immobilization of the molecule on titanium surfaces.
authors.affiliation: Department of Advanced Prosthodontics, Division of Cervico-Gnathostomatology, Hiroshima University Graduate School of Biomedical Sciences, Japan. abey@hiroshima-u.ac.jp
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Abe
authors.forename: Yohei
authors.initials: Y
authors.lastname: Okazaki
authors.forename: Kyou
authors.initials: K
authors.lastname: Hiasa
authors.forename: Isao
authors.initials: I
authors.lastname: Hirata
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Yoshida
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Taji
authors.forename: Kazuomi
authors.initials: K
authors.lastname: Suzuki
authors.forename: Masayuki
authors.initials: M
authors.lastname: Okazaki
authors.forename: Yasumasa
authors.initials: Y
authors.lastname: Akagawa
mesh_terms: D000327:Adsorption; D003764:Dental Materials / Q000737:chemistry*; D005353:Fibronectins / Q000737:chemistry*; D006801:Humans; D055767:Immobilized Proteins / Q000737:chemistry*; D008422:Materials Testing; D015394:Molecular Structure; D009842:Oligopeptides / Q000737:chemistry*; D010766:Phosphorylation; D010768:Phosphoserine / Q000737:chemistry*; D056951:Photoelectron Spectroscopy; D013499:Surface Properties; D013696:Temperature; D013997:Time Factors; D014025:Titanium / Q000737:chemistry*; D014465:Ultrasonics; D014867:Water / Q000737:chemistry
chemical_list: D003764:Dental Materials; D005353:Fibronectins; D055767:Immobilized Proteins; D009842:Oligopeptides; D014867:Water; D010768:Phosphoserine; C046617:arginyl-glycyl-aspartyl-serine; D014025:Titanium