title: Arsenic tolerances in rice (Oryza sativa) have a predominant role in transcriptional regulation of a set of genes including sulphur assimilation pathway and antioxidant system.
abstract: World wide arsenic (As) contamination of rice has raised much concern as it is the staple crop for millions. Four most commonly cultivated rice cultivars, Triguna, IR-36, PNR-519 and IET-4786, of the West Bengal region were taken for a hydroponic study to examine the effect of arsenate (As(V)) and arsenite (As(III)) on growth response, expression of genes and antioxidants vis-à-vis As accumulation. The rice genotypes responded differentially under As(V) and As(III) stress in terms of gene expression and antioxidant defences. Some of the transporters were up-regulated in all rice cultivars at lower doses of As species, except IET-4786. Phytochelatin synthase, GST and γ-ECS showed considerable variation in their expression pattern in all genotypes, however in IET-4786 they were generally down-regulated in higher As(III) stress. Similarly, most of antioxidants such as superoxide dismutase (SOD), ascorbate peroxidase (APX), guaiacol peroxidase (GPX), catalase (CAT), monodehydroascorbate reductase (MDHAR) and dehydroascorbate reductase (DHAR) increased significantly in Triguna, IR-36 and PNR-519 and decreased in IET-4786. Our study suggests that Triguna, IR-36 and PNR-519 are tolerant rice cultivars accumulating higher arsenic; however IET-4786 is susceptible to As-stress and accumulates less arsenic than other cultivars.
authors.affiliation: National Botanical Research Institute, Lucknow, UP, India.
authors.forename: Arti
authors.initials: A
authors.lastname: Rai
authors.forename: Preeti
authors.initials: P
authors.lastname: Tripathi
authors.forename: Sanjay
authors.initials: S
authors.lastname: Dwivedi
authors.forename: Sonali
authors.initials: S
authors.lastname: Dubey
authors.forename: Manju
authors.initials: M
authors.lastname: Shri
authors.forename: Smita
authors.initials: S
authors.lastname: Kumar
authors.forename: Pankaj Kumar
authors.initials: PK
authors.lastname: Tripathi
authors.forename: Richa
authors.initials: R
authors.lastname: Dave
authors.forename: Amit
authors.initials: A
authors.lastname: Kumar
authors.forename: Ragini
authors.initials: R
authors.lastname: Singh
authors.forename: Bijan
authors.initials: B
authors.lastname: Adhikari
authors.forename: Manas
authors.initials: M
authors.lastname: Bag
authors.forename: Rudra Deo
authors.initials: RD
authors.lastname: Tripathi
authors.forename: Prabodh K
authors.initials: PK
authors.lastname: Trivedi
authors.forename: Debasis
authors.initials: D
authors.lastname: Chakrabarty
authors.forename: Rakesh
authors.initials: R
authors.lastname: Tuli
mesh_terms: D000222:Adaptation, Physiological; D000975:Antioxidants / Q000378:metabolism; D001149:Arsenates / Q000378:metabolism / Q000633:toxicity; D001151:Arsenic / Q000378:metabolism / Q000633:toxicity*; D018053:Arsenites / Q000378:metabolism / Q000633:toxicity; D060387:Ascorbate Peroxidases; D002374:Catalase / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D017343:Genes, Plant; D008658:Inactivation, Metabolic / Q000235:genetics; D015227:Lipid Peroxidation / Q000187:drug effects; D012275:Oryza / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D018384:Oxidative Stress; D010088:Oxidoreductases / Q000378:metabolism; D010544:Peroxidases / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D012989:Soil Pollutants / Q000633:toxicity*; D013455:Sulfur / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D001149:Arsenates; D018053:Arsenites; D012989:Soil Pollutants; D013455:Sulfur; D010088:Oxidoreductases; D010544:Peroxidases; D060387:Ascorbate Peroxidases; D002374:Catalase; D013482:Superoxide Dismutase; C020666:glutathione dehydrogenase (ascorbate); C015001:arsenite; D001151:Arsenic; C025657:arsenic acid