title: Cell-based co-transfection microarrays for use with HEK293T cells on a poly D-lysine-coated polystyrene microplate.
abstract: Analysis of the human genome sequence has identified thousands of putative genes with unknown function; therefore, a new tool allowing for rapid identification of gene functions is needed. Reverse transfection microarray technology, which turns a DNA microarray into a cell-based microarray, has emerged for simultaneously studying the function of many genes. Since the initial demonstration in 2001, many variations have surfaced, making the technology more versatile for a broad range of applications. We have developed a protocol to make ready-to-transfect DNA microarrays in a 96-well microplate for co-transfection of two plasmids into HEK293T cells. This cell-based microarray in a microplate may be used for screening hundreds of analytes against multiple protein targets in parallel, providing a powerful tool for functional genomics and drug discovery.
authors.affiliation: Science and Technology Division, Corning Inc., Corning, NY, USA.
authors.forename: Meenal
authors.initials: M
authors.lastname: Soni
authors.forename: Fang
authors.initials: F
authors.lastname: Lai
mesh_terms: D020099:Coated Materials, Biocompatible / Q000378:metabolism; D005796:Genes; D015894:Genome, Human; D057809:HEK293 Cells; D006801:Humans; D008239:Lysine / Q000235:genetics / Q000378:metabolism; D010641:Phenotype; D010957:Plasmids; D011137:Polystyrenes / Q000378:metabolism*; D011506:Proteins / Q000235:genetics / Q000378:metabolism; D046888:Tissue Array Analysis / Q000379:methods*; D014162:Transfection
chemical_list: D020099:Coated Materials, Biocompatible; D011137:Polystyrenes; D011506:Proteins; D008239:Lysine