title: Noncovalent functionalization of carbon nanotubes with lectin for label-free dynamic monitoring of cell-surface glycan expression.
abstract: A kind of concanavalin A functionalized multiwalled carbon nanotube (ConA-MWCNT) was constructed by noncovalent assembly of ConA on carboxylated MWCNT with poly(diallyldimethylammonium) as a linker. The novel nanomaterial was characterized with scanning electron microscopy and atomic force microscopy. It incorporated both the specific recognition ability of lectin for cell-surface mannosyl groups and the unique electronic and mechanical properties of MWCNT. An electrochemical label-free method for cytosensing was proposed by constructing a ConA-MWCNT interface on a glassy carbon electrode, which showed a linear response to K562 cells ranging from 1 × 10(4) to 1 × 10(7) cellsmL(-1). The ConA-MWCNT interface could be further used for monitoring of dynamic variation of glycan expression on K562 cells in response to drugs. A facile and high-throughput optical method for the analysis of dynamic glycan expression on living cells was also developed by constructing an array of ConA-MWCNT spots on a glass slide. This method showed acceptable rapidity and low cost. The noncovalent functionalization of MWCNTs with lectins could be potentially applied in cell biological studies based on cell-surface glycan expression.
authors.affiliation: Key Laboratory of Analytical Chemistry for Life Science (Ministry of Education of China), Department of Chemistry, Nanjing University, Nanjing 210093, People's Republic of China.
authors.forename: Yadong
authors.initials: Y
authors.lastname: Xue
authors.forename: Lei
authors.initials: L
authors.lastname: Bao
authors.forename: Xirui
authors.initials: X
authors.lastname: Xiao
authors.forename: Lin
authors.initials: L
authors.lastname: Ding
authors.forename: Jianping
authors.initials: J
authors.lastname: Lei
authors.forename: Huangxian
authors.initials: H
authors.lastname: Ju
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D002417:Cattle; D018914:Cells, Immobilized / Q000378:metabolism*; D004563:Electrochemistry; D004566:Electrodes; D005786:Gene Expression Regulation; D005898:Glass / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D020014:K562 Cells; D037102:Lectins / Q000737:chemistry*; D008567:Membranes, Artificial; D037742:Nanotubes, Carbon / Q000737:chemistry*; D055620:Optical Phenomena; D011095:Polyethylenes / Q000737:chemistry; D011134:Polysaccharides / Q000378:metabolism*; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D015203:Reproducibility of Results
chemical_list: D037102:Lectins; D008567:Membranes, Artificial; D037742:Nanotubes, Carbon; D011095:Polyethylenes; D011134:Polysaccharides; D000644:Quaternary Ammonium Compounds; C041004:poly-N,N-dimethyl-N,N-diallylammonium chloride