title: Photosensitizer loaded HSA nanoparticles II: in vitro investigations.
abstract: The photosensitizing efficiency of human serum albumin (HSA) nanoparticles loaded with the photosensitizers meta-tetra(hydroxy-phenyl)-chlorin (mTHPC) and meta-tetra(hydroxy-phenyl)-porphyrin (mTHPP) was investigated in vitro. The endocytotic intracellular uptake, and the time dependent drug release caused by nanoparticle decomposition of the PS loaded HSA nanoparticles were studied on Jurkat cells in suspension. The photoxicity as well as the intracellular singlet oxygen ((1)O(2)) generation were investigated in dependence on the incubation time. The obtained results show that HSA nanoparticles are promising carriers for the clinical used mTHPC (Foscan). After release the ((1)O(2)) generation as well as the phototoxicity are more efficient compared with mTHPC applied without the HSA nanoparticles.
authors.affiliation: Department of Physics, Humboldt - Universität zu Berlin, Newtonstraße 15, 12489 Berlin, Germany.
authors.forename: Annegret
authors.initials: A
authors.lastname: Preuss
authors.forename: Kuan
authors.initials: K
authors.lastname: Chen
authors.forename: Steffen
authors.initials: S
authors.lastname: Hackbarth
authors.forename: Matthias
authors.initials: M
authors.lastname: Wacker
authors.forename: Klaus
authors.initials: K
authors.lastname: Langer
authors.forename: Beate
authors.initials: B
authors.lastname: Röder
mesh_terms: D002626:Chemistry, Pharmaceutical; D004337:Drug Carriers; D004339:Drug Compounding; D004705:Endocytosis; D006801:Humans; D019169:Jurkat Cells; D007700:Kinetics; D008652:Mesoporphyrins / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D018613:Microscopy, Confocal; D053758:Nanoparticles; D036103:Nanotechnology; D017319:Photosensitizing Agents / Q000737:chemistry*; D011166:Porphyrins / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D012709:Serum Albumin / Q000737:chemistry*; D026082:Singlet Oxygen / Q000378:metabolism; D012995:Solubility; D013678:Technology, Pharmaceutical / Q000379:methods
chemical_list: D004337:Drug Carriers; D008652:Mesoporphyrins; D017319:Photosensitizing Agents; D011166:Porphyrins; D012709:Serum Albumin; C437342:tetra(3-hydroxyphenyl)porphine; D026082:Singlet Oxygen; C072269:temoporfin