title: A bioactive self-assembled membrane to promote angiogenesis.
abstract: We report here on a bioactive hierarchically structured membrane formed by self-assembly. The membrane is formed with hyaluronic acid and peptide amphiphiles with binding affinity for heparin, and its hierarchical structure contains both an amorphous zone and a layer of fibrils oriented perpendicular to the membrane plane. The design of bioactivity is based on the potential ability to bind and slowly release heparin-binding growth factors. Human mesenchymal stem cells (hMSCs) seeded on these membranes attached and remained viable. Basic fibroblast growth factor (FGF2) and vascular endothelial growth factor (VEGF) were incorporated within the membrane structure prior to self-assembly and released into media over a prolonged period of time (14 days). Using the chicken chorioallantoic membrane (CAM) assay, we also found that these membranes induced a significant and rapid enhancement of angiogenesis relative to controls.
authors.affiliation: Department of Materials Science and Engineering, Northwestern University, Evanston, IL 60208, United States.
authors.forename: Lesley W
authors.initials: LW
authors.lastname: Chow
authors.forename: Ronit
authors.initials: R
authors.lastname: Bitton
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Webber
authors.forename: Daniel
authors.initials: D
authors.lastname: Carvajal
authors.forename: Kenneth R
authors.initials: KR
authors.lastname: Shull
authors.forename: Arun K
authors.initials: AK
authors.lastname: Sharma
authors.forename: Samuel I
authors.initials: SI
authors.lastname: Stupp
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002642:Chick Embryo; D002645:Chickens; D049033:Chorioallantoic Membrane / Q000166:cytology*; D016222:Fibroblast Growth Factor 2 / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D042461:Vascular Endothelial Growth Factor A / Q000737:chemistry / Q000494:pharmacology
chemical_list: D001672:Biocompatible Materials; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D016222:Fibroblast Growth Factor 2; D006820:Hyaluronic Acid