title: Multiplexed DNA sequence capture of mitochondrial genomes using PCR products.
abstract: BACKGROUND
  To utilize the power of high-throughput sequencers, target enrichment methods have been developed. The majority of these require reagents and equipment that are only available from commercial vendors and are not suitable for the targets that are a few kilobases in length.
  
  
  RESULTS
  We describe a novel and economical method in which custom made long-range PCR products are used to capture complete human mitochondrial genomes from complex DNA mixtures. We use the method to capture 46 complete mitochondrial genomes in parallel and we sequence them on a single lane of an Illumina GA(II) instrument.
  
  
  CONCLUSIONS
  This method is economical and simple and particularly suitable for targets that can be amplified by PCR and do not contain highly repetitive sequences such as mtDNA. It has applications in population genetics and forensics, as well as studies of ancient DNA.
authors.affiliation: Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Leipzig, Germany. tomislav_maricic@eva.mpg.de
authors.forename: Tomislav
authors.initials: T
authors.lastname: Maricic
authors.forename: Mark
authors.initials: M
authors.lastname: Whitten
authors.forename: Svante
authors.initials: S
authors.lastname: Pääbo
mesh_terms: D004272:DNA, Mitochondrial / Q000737:chemistry / Q000235:genetics*; D053803:Forensic Genetics / Q000379:methods; D005828:Genetics, Population / Q000379:methods; D054629:Genome, Mitochondrial / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000295:instrumentation / Q000379:methods*; D015203:Reproducibility of Results; D017422:Sequence Analysis, DNA / Q000295:instrumentation / Q000379:methods*
chemical_list: D004272:DNA, Mitochondrial