title: Complete nucleotide sequence and evolutionary analysis of a gorilla foamy virus.
abstract: To shed light on primate foamy virus (FV) evolution, we determined the complete nucleotide sequence of the gorilla simian foamy virus (SFVgor). Starting from a conserved region in the integrase (IN) domain of the pol gene we cloned the viral genome to the 5' and 3' LTR into plasmid vectors and elucidated its nucleotide sequence. The sequences of both LTRs were determined by nucleotide sequencing of separate PCR products from the primer-binding site or the bel region and LTRs. All protein motifs conserved among the primate FV were identified in SFVgor. Using phylogenetic analysis of the Gag, Pol and Env amino acid sequences, we demonstrate that SFVgor consistently clusters in accordance with a scenario of virus-host co-divergence.
authors.affiliation: Institute of Virology and Immunobiology, University of Würzburg, Germany.
authors.forename: Andrea
authors.initials: A
authors.lastname: Schulze
authors.forename: Philippe
authors.initials: P
authors.lastname: Lemey
authors.forename: Jörg
authors.initials: J
authors.lastname: Schubert
authors.forename: Myra O
authors.initials: MO
authors.lastname: McClure
authors.forename: Axel
authors.initials: A
authors.lastname: Rethwilm
authors.forename: Jochen
authors.initials: J
authors.lastname: Bodem
mesh_terms: D020816:Amino Acid Motifs; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D016000:Cluster Analysis; D017124:Conserved Sequence; D015686:Gene Products, env / Q000235:genetics; D015683:Gene Products, gag / Q000235:genetics; D015684:Gene Products, pol / Q000235:genetics; D005822:Genetic Vectors; D016679:Genome, Viral; D006071:Gorilla gorilla / Q000821:virology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D010957:Plasmids; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D053150:Simian foamy virus / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D015686:Gene Products, env; D015683:Gene Products, gag; D015684:Gene Products, pol; D012367:RNA, Viral