title: Genotyping and phylogenetic analysis of the major genes in respiratory syncytial virus isolated from infants with bronchiolitis.
abstract: We performed the genotyping and phylogenetic analysis of respiratory syncytial virus (RSV) isolated from 17 infants with bronchiolitis in Kanagawa Prefecture, Japan in 2005 and 2006. The major genes in these samples (attachment [G] glycoprotein gene, fusion [F] protein gene, and nucleoprotein [N] gene) were sequenced and analyzed genetically. Phylogenetic analysis of these genes revealed that 7 and 10 strains could be classified into subgroups A and B, respectively. Phylogenetic analysis of the G gene revealed that the subgroup A and B strains were unique genotypes GA2 and BA, respectively. Moreover, the amino acid sequences for these genotypes suggested a relatively high frequency of amino acid substitutions in the G and F proteins in these strains, whereas the N protein was highly homologous. These results suggest that RSV genotypes GA2 and BA may be associated with bronchiolitis in the cases studied here.
authors.affiliation: National Hospital Organization Yokohama Medical Center, Kanagawa 245-8575, Japan.
authors.forename: Kazuko
authors.initials: K
authors.lastname: Goto-Sugai
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Tsukagoshi
authors.forename: Katsumi
authors.initials: K
authors.lastname: Mizuta
authors.forename: Shunji
authors.initials: S
authors.lastname: Matsuda
authors.forename: Masahiro
authors.initials: M
authors.lastname: Noda
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Sugai
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Saito
authors.forename: Nobuhiko
authors.initials: N
authors.lastname: Okabe
authors.forename: Masato
authors.initials: M
authors.lastname: Tashiro
authors.forename: Kunihisa
authors.initials: K
authors.lastname: Kozawa
authors.forename: Ryota
authors.initials: R
authors.lastname: Tanaka
authors.forename: Yukio
authors.initials: Y
authors.lastname: Morita
authors.forename: Atsuyoshi
authors.initials: A
authors.lastname: Nishina
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Kimura
mesh_terms: D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D001990:Bronchiolitis, Viral / Q000821:virology*; D005838:Genotype; D006801:Humans; D007223:Infant; D007564:Japan; D008969:Molecular Sequence Data; D019590:Nucleocapsid Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D010802:Phylogeny; D018357:Respiratory Syncytial Virus Infections / Q000821:virology*; D018113:Respiratory Syncytial Virus, Human / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D017422:Sequence Analysis, DNA; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D014760:Viral Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
chemical_list: C526850:F protein, human respiratory syncytial virus; D019590:Nucleocapsid Proteins; D014759:Viral Envelope Proteins; D014760:Viral Fusion Proteins; D014764:Viral Proteins; C116204:attachment protein G