title: Envelope protein E1 as vaccine target for western equine encephalitis virus.
abstract: Western equine encephalitis virus (WEEV) is a mosquito-borne RNA virus which causes lethal infection in humans and equines. There are no commercial vaccines or anti-WEEV drugs available for humans. We used replication-defective, human adenovirus serotype-5 (HAd5) as a delivery vector for developing WEEV vaccine. Our previous study found delivery of both E1 and E2 envelope proteins of WEEV by HAd5 vector offers complete protection against lethal challenge of WEEV. In this paper, we constructed a HAd5-vectored E1 vaccine, Ad5-E1. Mice given single-dose vaccination of Ad5-E1 were completely protected against both homologous and heterologous WEEV strains. The protection was rapid, which was achieved as early as day 7 after vaccination. In addition, Ad5-E1 induced a strong WEEV-specific T cell response. Our data suggest E1 is a potential target for developing single-dose, fast-acting, HAd5-vectored vaccine for WEEV.
authors.affiliation: Defence Research and Development Canada - Suffield, Box 4000, Station Main, Medicine Hat, Alberta T1A 8K6, Canada.
authors.forename: Richard D
authors.initials: RD
authors.lastname: Swayze
authors.forename: Hardeep S
authors.initials: HS
authors.lastname: Bhogal
authors.forename: Nicole D
authors.initials: ND
authors.lastname: Barabé
authors.forename: Lori J
authors.initials: LJ
authors.lastname: McLaws
authors.forename: Josh Q H
authors.initials: JQ
authors.lastname: Wu
mesh_terms: D000260:Adenoviruses, Human / Q000235:genetics; D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood; D004195:Disease Models, Animal; D004337:Drug Carriers; D004667:Encephalitis Virus, Western Equine / Q000276:immunology*; D004683:Encephalomyelitis, Equine / Q000276:immunology / Q000517:prevention & control*; D005260:Female; D005822:Genetic Vectors; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D016019:Survival Analysis; D013601:T-Lymphocytes / Q000276:immunology; D014759:Viral Envelope Proteins / Q000276:immunology*; D010948:Viral Plaque Assay; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D004337:Drug Carriers; D014759:Viral Envelope Proteins; D014765:Viral Vaccines