title: A novel bombesin-like peptide from skin of Rana shuchinae.
abstract: Bombesin and its receptors have been demonstrated to be involved in a larger array of physiological and pathophysiological conditions including memory and fear behavior, lung development and injury, and tumor growth. A bombesin-like peptide named bombesin-RS was purified and characterized from the skin secretions of the frog, Rana shuchinae. Its amino acid sequence (pETSFMAPSWALGHLM-NH2) was determined by auto Edman degradation and mass spectrometry. The cDNA encoding bombesin-RS precursor was cloned from skin cDNA library of this frog. The precursor is composed of 67 amino acid residues including a copy of mature bombesin-RS. Two predicted enzymatic processing sites (-Lys-Lys- and -Lys-Arg-) are flanked at the mature bombesin-RS sequence. In the in vitro myotropic contraction experiment, the synthesized bombesin-RS displayed stimulating effect toward rat stomach strips, suggesting that bombesin-RS acts as agonist as most other bombesin-related peptides do.
authors.affiliation: Department of General Surgery, Nanjing First Hospital Affiliated to Nanjing Medical University, Nanjing, 210006, Jiangsu, China. h.xwan@yahoo.com
authors.forename: Hanjin
authors.initials: H
authors.lastname: Wang
authors.forename: Jianmin
authors.initials: J
authors.lastname: Bian
authors.forename: Zhenwen
authors.initials: Z
authors.lastname: Chen
authors.forename: Yinglei
authors.initials: Y
authors.lastname: Miao
authors.forename: Wenliang
authors.initials: W
authors.lastname: Li
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001839:Bombesin / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000494:pharmacology; D018076:DNA, Complementary / Q000235:genetics; D066298:In Vitro Techniques; D008297:Male; D008969:Molecular Sequence Data; D009119:Muscle Contraction / Q000187:drug effects; D009132:Muscles / Q000187:drug effects / Q000502:physiology; D011898:Ranidae; D051381:Rats; D017208:Rats, Wistar; D012867:Skin / Q000737:chemistry* / Q000378:metabolism; D013270:Stomach / Q000187:drug effects / Q000502:physiology
chemical_list: D018076:DNA, Complementary; D001839:Bombesin