title: Electrochemical detection of peroxynitrite using a biosensor based on a conducting polymer-manganese ion complex.
abstract: A peroxynitrite (ONOO(-)) biosensor has been developed through the preparation of a new manganese-[poly-2,5-di-(2-thienyl)-1H-pyrrole)-1-(p-benzoic acid)] (Mn-pDPB) complex. DPB monomer was first synthesized and polymerized for the purpose of providing a polymer backbone for complex formation with Mn(2+) ion. The Mn-pDPB complex was characterized via Magnetomotive Force (MMF) simulation, X-ray photoelectron spectroscopy (XPS), and cyclic voltammetry. The complex selectively enhanced the reduction process of ONOO(-) which was used as the analytical signal for chronoamperometric detection. A polyethyleneimmine (PEI) layer was coated on the complex surface to increase selectivity and stability. The chronoamperometric calibration plot showed the hydrodynamic range of 2.0 × 10(-8)-5.0 × 10(-7) M. The detection limit was determined to be 1.9 (±0.2) × 10(-9) M based on S/N = 3. The microbiosensor, fabricated on a 100 μm diameter Pt tip, was applied in a real rat plasma sample for the detection of spiked concentrations of ONOO(-). The reliability and long-term stability of the microbiosensor was also examined with YPEN-1 cells in vitro, and the results shown were promising.
authors.affiliation: Department of Chemistry, Institute of BioPhysio Sensor Technology, Pusan National University, Busan 609-735, Korea.
authors.forename: Wei Choon Alvin
authors.initials: WC
authors.lastname: Koh
authors.forename: Jung Ik
authors.initials: JI
authors.lastname: Son
authors.forename: Eun Sang
authors.initials: ES
authors.lastname: Choe
authors.forename: Yoon-Bo
authors.initials: YB
authors.lastname: Shim
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D004553:Electric Conductivity; D055664:Electrochemical Techniques / Q000379:methods*; D008345:Manganese / Q000737:chemistry; D030421:Peroxynitrous Acid / Q000032:analysis*; D011108:Polymers / Q000737:chemistry
chemical_list: D011108:Polymers; D030421:Peroxynitrous Acid; D008345:Manganese