title: Impact on clinical decision making of quality control standards applied to sputum analysis in COPD.
abstract: OBJECTIVE
  Sputum analysis is important in COPD exacerbation management. We determined whether application of stringent quality control criteria for sputum samples had an impact on culture results.
  
  
  METHODS
  We analyzed sputum samples of 108 patients during stable COPD and during exacerbations. To all samples quality control standards and culture interpretation rules according to the American Society of Microbiologists (ASM) were applied.
  
  
  RESULTS
  In sputum exacerbation samples considered appropriate according to ASM quality standards, criteria for infection (40%) were met more often compared to inappropriate samples (13%) (p < 0.001). The same pattern was observed when applying these rules to sputum samples obtained during stable disease, (50% vs. 18%, p < 0.001). There was no difference in the percentage of infectious cultures obtained during the stable state and exacerbations.
  
  
  CONCLUSIONS
  Applying stringent quality control criteria to sputum samples can have a profound effect on the labeling of sputum samples as infectious, and therefore on clinical decision making.
authors.affiliation: Department of Pulmonary Medicine, Medisch Spectrum Twente, PO Box 50 000, 7500 KA Enschede, the Netherlands.
authors.forename: M C
authors.initials: MC
authors.lastname: Telgen
authors.forename: M G J
authors.initials: MG
authors.lastname: Brusse-Keizer
authors.forename: P D L P M
authors.initials: PD
authors.lastname: van der Valk
authors.forename: J
authors.initials: J
authors.lastname: van der Palen
authors.forename: H A M
authors.initials: HA
authors.lastname: Kerstjens
authors.forename: M G R
authors.initials: MG
authors.lastname: Hendrix
mesh_terms: D000328:Adult; D000368:Aged; D019411:Clinical Laboratory Techniques; D003657:Decision Making; D005260:Female; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests / Q000379:methods / Q000592:standards*; D008875:Middle Aged; D029424:Pulmonary Disease, Chronic Obstructive / Q000382:microbiology*; D011786:Quality Control; D013048:Specimen Handling / Q000592:standards*; D013183:Sputum / Q000382:microbiology*