title: Targeting antibodies to the cytoplasm.
abstract: A growing number of research consortia are now focused on generating antibodies and recombinant antibody fragments that target the human proteome. A particularly valuable application for these binding molecules would be their use inside a living cell, e.g., for imaging or functional intervention. Animal-derived antibodies must be brought into the cell through the membrane, whereas the availability of the antibody genes from phage display systems allows intracellular expression. Here, the various technologies to target intracellular proteins with antibodies are reviewed.
authors.affiliation: Technische Universität Braunschweig; Institute of Biochemistry and Biotechnology; Braunschweig, Germany.
authors.forename: Andrea L J
authors.initials: AL
authors.lastname: Marschall
authors.forename: André
authors.initials: A
authors.lastname: Frenzel
authors.forename: Thomas
authors.initials: T
authors.lastname: Schirrmann
authors.forename: Manuela
authors.initials: M
authors.lastname: Schüngel
authors.forename: Stefan
authors.initials: S
authors.lastname: Dübel
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism*; D004705:Endocytosis; D006801:Humans; D008969:Molecular Sequence Data; D017510:Protein Folding; D021381:Protein Transport; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D000906:Antibodies; D011994:Recombinant Proteins