title: Evaluating and defining sample preparation procedures for DBS LC-MS/MS assays.
abstract: BACKGROUND
  A defined approach to develop and validate an LC-MS/MS assay using dried blood spot (DBS) samples is of great interest to many scientists who are adopting this technology. We have evaluated three distinct sample preparation procedures of DBS samples for LC-MS/MS assay development.
  
  
  RESULTS
  A new term 'elution efficiency' is introduced to evaluate the effectiveness of eluting compounds from the DBS cards into the liquid phase. Three different types of DBS cards were studied as part of the sample preparation procedures. A DBS LC-MS/MS method was developed, qualified and then applied to a toxicokinetics study.
  
  
  CONCLUSIONS
  Organic extraction and protein precipitation resulted in significant ion suppression and/or enhancement for FTA(®) Classic or FTA(®) Elute cards. Liquid-liquid extraction produced the least ion suppression/enhancement. Both protein precipitation and liquid-liquid extraction effectively eluted the probe compound from the DBS cards under the conditions tested. However, organic extraction by pure solvents resulted in low elution efficiency.
authors.affiliation: Bioanalytical and Discovery Bioanalytical Sciences, Bristol-Myers Squibb Co., Rte 206 and Province Line Road, Princeton, NJ 08543, USA.
authors.forename: Guowen
authors.initials: G
authors.lastname: Liu
authors.forename: Laura
authors.initials: L
authors.lastname: Patrone
authors.forename: Heidi M
authors.initials: HM
authors.lastname: Snapp
authors.forename: Alicja
authors.initials: A
authors.lastname: Batog
authors.forename: Jack
authors.initials: J
authors.lastname: Valentine
authors.forename: Greg
authors.initials: G
authors.lastname: Cosma
authors.forename: Adrienne
authors.initials: A
authors.lastname: Tymiak
authors.forename: Qin C
authors.initials: QC
authors.lastname: Ji
authors.forename: Mark E
authors.initials: ME
authors.lastname: Arnold
mesh_terms: D053000:Analytic Sample Preparation Methods / Q000379:methods*; D000818:Animals; D001774:Blood Chemical Analysis / Q000379:methods*; D001798:Blood Proteins / Q000737:chemistry; D001800:Blood Specimen Collection / Q000379:methods*; D011232:Chemical Precipitation; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003890:Desiccation; D010599:Pharmacokinetics; D051381:Rats; D053719:Tandem Mass Spectrometry / Q000379:methods*; D009626:Terminology as Topic
chemical_list: D001798:Blood Proteins