title: Single channel properties of lysenin measured in artificial lipid bilayers and their applications to biomolecule detection.
abstract: Single channel currents of lysenin were measured using artificial lipid bilayers formed on a glass micropipette tip. The single channel conductance for KCl, NaCl, CaCl(2), and Trimethylammonium-Cl were 474 ± 87, 537 ± 66, 210 ± 14, and 274 ± 10 pS, respectively, while the permeability ratio P(Na)/P(Cl) was 5.8. By adding poly(deoxy adenine) or poly(L-lysine) to one side of the bilayer, channel currents were influenced when membrane voltages were applied to pass the charged molecules through the channel pores. Current inhibition process was concentration-dependent with applied DNA. As the current fluctuations of α-hemolysin channels is often cited as the detector in a molecular sensor, these results suggest that by monitoring channel current changes, the lysenin channel has possibilities to detect interactions between it and certain biomolecules by its current fluctuations.
authors.affiliation: Network Center for Molecular and System Life Science, Graduate School of Frontier Biosciences, Osaka University, Osaka, Japan.
authors.forename: Takaaki
authors.initials: T
authors.lastname: Aoki
authors.forename: Minako
authors.initials: M
authors.lastname: Hirano
authors.forename: Yuko
authors.initials: Y
authors.lastname: Takeuchi
authors.forename: Toshihide
authors.initials: T
authors.lastname: Kobayashi
authors.forename: Toshio
authors.initials: T
authors.lastname: Yanagida
authors.forename: Toru
authors.initials: T
authors.lastname: Ide
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D004560:Electricity; D015640:Ion Channel Gating / Q000187:drug effects; D008051:Lipid Bilayers / Q000378:metabolism*; D008239:Lysine / Q000378:metabolism*; D046911:Macromolecular Substances / Q000494:pharmacology
chemical_list: D008051:Lipid Bilayers; D046911:Macromolecular Substances; D008239:Lysine