title: [Effect of heat treatment on the antigenicity and conformation of peanut allergen Ara h 2].
abstract: Peanut allergen Ara h 2 was extracted from peanuts and was identified by SDS-PAGE and MALDI-TOF-MS. Effect of heat treatment on the antigenicity and structure of Ara h 2 was measured by indirect ELISA, CD, fluorescence and UV absorption spectra. The results showed that the antigenicity of Ara h 2 had a slight increase after being heated at 55 or 70 degrees C, while above 85 degrees C the antigenicity decreased significantly, and the antigeicity of Ara h 2 decreased with increasing temperature. The CD showed that the secondary structure of Ara h 2 was changed after heat treatment. The ANS fluorescence probe emission spectra analysis demonstrated that the heat treatment induced an increase in surface hydrophobicity of Ara h 2. The UV absorption spectra showed that the absorption maximum wavelength was increased when Ara h 2 was heated except the sample heating at 50 degrees C for 30 min. So the changes in conformation of Ara h 2 lead to the antigenicity degression.
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China. hchqiu@yahoo.com.cn
authors.forename: Chun-qiu
authors.initials: CQ
authors.lastname: Hu
authors.forename: Jin-yan
authors.initials: JY
authors.lastname: Gao
authors.forename: Hong-bing
authors.initials: HB
authors.lastname: Chen
authors.forename: Chun-ping
authors.initials: CP
authors.lastname: Luo
authors.forename: Fei
authors.initials: F
authors.lastname: Yan
mesh_terms: D055516:2S Albumins, Plant / Q000276:immunology*; D052179:Antigens, Plant / Q000276:immunology*; D010367:Arachis / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006023:Glycoproteins / Q000276:immunology*; D006358:Hot Temperature; D017433:Protein Structure, Secondary
chemical_list: D055516:2S Albumins, Plant; D052179:Antigens, Plant; C500890:Ara h 2 allergen, Arachis hypogaea; D006023:Glycoproteins