title: Effects of iRoot SP on mineralization-related genes expression in MG63 cells.
abstract: BACKGROUND
  The purpose of this study was to assess the effects of iRoot SP root canal sealer (Innovative BioCreamix Inc, Vancouver, Canada) on the expression of mineralization-related genes in human MG63 osteoblast-like cells.
  
  
  METHODS
  Specimens (5 mm in diameter and 2 mm in height) of iRoot SP and AH Plus (Dentsply DeTrey, Konstanz, Germany) were extracted from a 5-mL culture medium. The MG63 cells were exposed to various dilutions (1/1, 1/2, and 1/4) of the extracts. The 3,(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide MTT assay was used for assessing the dental materials' nonspecific cytotoxicity. The expression of mineralization-related genes, including collagen type I (COL I), osteocalcin (OCN), bone sialoprotein (BSP) and osteopontin (OPN), was detected on days 1, 3, and 6 by a real-time polymerase chain reaction. An enzyme-linked immunosorbent assay experiment was used for evaluating COL I and BSP protein changes. The data were analyzed with one-way analysis of variance and Tukey tests.
  
  
  RESULTS
  In the MTT assay, the undiluted extracts of iRoot SP were noncytotoxic, whereas the undiluted extracts of AH Plus were rated as slightly cytotoxic. iRoot SP up-regulated COL I, OCN, and BSP messenger RNA expression after 3 and 6 days. In the enzyme-linked immunosorbent assay experiment, iRoot SP increased COL I and BSP protein levels compared with AH Plus and the control group on day 6.
  
  
  CONCLUSIONS
  In the presence of iRoot SP, MG63 cells can produce more mineralized matrix gene and protein expression. Based on these results, iRoot SP can be considered as a favorable material for cell-material interaction.
authors.affiliation: Department of Operative Dentistry and Endodontics, School of Stomatology, Wuhan University,Wuhan, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Zhi
authors.initials: Z
authors.lastname: Li
authors.forename: Bin
authors.initials: B
authors.lastname: Peng
mesh_terms: D000704:Analysis of Variance; D002113:Calcification, Physiologic / Q000187:drug effects / Q000235:genetics*; D017610:Calcium Compounds / Q000494:pharmacology / Q000633:toxicity; D002460:Cell Line; D024042:Collagen Type I / Q000096:biosynthesis / Q000235:genetics; D004396:Coloring Agents / Q000378:metabolism; D004853:Epoxy Resins / Q000494:pharmacology / Q000633:toxicity; D015870:Gene Expression / Q000187:drug effects*; D006801:Humans; D058574:Integrin-Binding Sialoprotein / Q000096:biosynthesis / Q000235:genetics; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism; D015675:Osteocalcin / Q000096:biosynthesis / Q000235:genetics; D053495:Osteopontin / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis; D012387:Root Canal Filling Materials / Q000494:pharmacology* / Q000633:toxicity; D012823:Silicate Cement / Q000494:pharmacology / Q000633:toxicity; D017640:Silicates / Q000494:pharmacology / Q000633:toxicity; D018709:Statistics, Nonparametric; D013778:Tetrazolium Salts / Q000378:metabolism; D013844:Thiazoles / Q000378:metabolism
chemical_list: D017610:Calcium Compounds; D024042:Collagen Type I; D004396:Coloring Agents; D004853:Epoxy Resins; D058574:Integrin-Binding Sialoprotein; D012333:RNA, Messenger; D012387:Root Canal Filling Materials; D017640:Silicates; D013778:Tetrazolium Salts; D013844:Thiazoles; C534916:epoxy resin-based root canal sealer; D015675:Osteocalcin; D053495:Osteopontin; D012823:Silicate Cement; C022616:thiazolyl blue; C031293:calcium silicate