title: Morphine decreases early peritoneal innate immunity responses in Swiss-Webster and C57BL6/J mice through the inhibition of mast cell TNF-α release.
abstract: OBJECTIVE
  To characterize immunosuppressive effects of morphine on the early innate immunity response of cytokine production in peritoneal cavity after LPS challenge.
  
  
  METHODS
  The effects of a single i.p. administration of morphine (3.1 or 31 mg/kg) on LPS-induced tumor necrosis factor α (TNF-α) and monocyte chemoattractant protein-1 (CCL2) intraperitoneal release was tested in Swiss-Webster, C57BL/6J, mast cell deficient Kit(Wsh/Wsh) (W-sh) and mast cell reconstituted (W-sh-rec) mice.
  
  
  RESULTS
  Morphine was found to inhibit LPS-induced TNF-α but not CCL2 release in the peritoneal cavity. Studies on mast cell deficient and reconstituted mice indicate that resident mast cells mediate selective morphine immunosuppression in the peritoneal cavity.
authors.affiliation: Departamento de Farmacobiologia, Cinvestav, IPN Calzada de los Tenorios 235, Col. Granjas Coapa, Mexico, D.F. 14330, Mexico.
authors.forename: Iris K
authors.initials: IK
authors.lastname: Madera-Salcedo
authors.forename: Silvia L
authors.initials: SL
authors.lastname: Cruz
authors.forename: Claudia
authors.initials: C
authors.lastname: Gonzalez-Espinosa
mesh_terms: D000818:Animals; D002469:Cell Separation; D018932:Chemokine CCL2 / Q000187:drug effects / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D007113:Immunity, Innate / Q000187:drug effects*; D008070:Lipopolysaccharides / Q000276:immunology; D008297:Male; D008407:Mast Cells / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D009020:Morphine / Q000494:pharmacology*; D009294:Narcotics / Q000494:pharmacology*; D010529:Peritoneal Cavity / Q000166:cytology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: C491768:Ccl2 protein, mouse; D018932:Chemokine CCL2; D008070:Lipopolysaccharides; D009294:Narcotics; D014409:Tumor Necrosis Factor-alpha; D009020:Morphine