title: [Effects of acupoint-injection of Lentinan on the immunologic funtion in rabbits with spleen-qi deficiency].
abstract: To observe the effect of Spleen-Meridian-acupoint injection of Lentinan on the immunologic function in spleen-deficiency rabbits.
  
  
  METHODS
  A total of 54 Newzealand rabbits were randomly divided into normal (n = 10), model (n = 8), intramuscular injection (n = 10), Sanyinjiao (SP6, n = 10), Diji (SP8, n = 8) and Xuehai (SP 12, n = 8) groups. Spleen-qi deficiency model was established by intragastric administration of 100% crude Radix et Rhizoma Rhei decoction (15 mL/kg/day x 10 d), and then Lentinan (LNT, 0.025 mg/kg/2 day x 5) was injected into the aforementioned acupoints of the Spleen Meridian. The erythrocyte immunologic function (RBC-C3 bR, RBC-IC), hemolysin (lgM) and changes of physical signs of the rabbits were observed.
  
  
  RESULTS
  In comparison with the control group, the rabbits' body weight, rectal temperature, RBC-C3 bR% and serum IgM level were decreased significantly in model group (P < 0.05); while in comparison with the model group, the body weight in SP 8 group,retal temperature in SP 9 and SP 8 groups, RBC-C3 bR% in SP 9 and SP 12 groups, and serum IgM levels in SP 9, SP 8 and SP 12 groups increased considerably (P < 0.05, P < 0.01). Comparison among the 4 treatment groups showed that the effect of SP 12 was superior to that of intramuscular injection group in upregulaing RBC-C3 bR%, and the effects of SP 9, SP 8 and SP 12 groups were significantly superior to those of intramuscular injection group in upregulating serum IgM level (P < 0.05).
  
  
  CONCLUSIONS
  The Spleen-Meridian-acupoint injection of LNT is superior to that of intramuscular injection of LNT in improving the spleen-qi deficiency rabbits' symptoms and immunologic function.
authors.affiliation: Department of Drugs, Fujian Institute of Chinese Medicine, Fuzhou, China. ljy7716@tom.com
authors.forename: Jing-Yu
authors.initials: JY
authors.lastname: Lin
authors.forename: Yun-Lu
authors.initials: YL
authors.lastname: Xu
authors.forename: Xin
authors.initials: X
authors.lastname: Yao
authors.forename: Xiang-Long
authors.initials: XL
authors.lastname: Hu
authors.forename: Ni
authors.initials: N
authors.lastname: Feng
mesh_terms: D015669:Acupuncture Points; D000818:Animals; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D006801:Humans; D007267:Injections; D007912:Lentinan / Q000008:administration & dosage*; D008297:Male; D011817:Rabbits; D011897:Random Allocation; D013154:Spleen / Q000187:drug effects / Q000276:immunology* / Q000503:physiopathology; D013158:Splenic Diseases / Q000188:drug therapy / Q000276:immunology* / Q000503:physiopathology
chemical_list: D004365:Drugs, Chinese Herbal; D007912:Lentinan