title: Molecular cloning, expression profile and 5' regulatory region analysis of two chemosensory protein genes from the diamondback moth, Plutella xylostella.
abstract: Chemosensory proteins play an important role in transporting chemical compounds to their receptors on dendrite membranes. In this study, two full-length cDNA codings for chemosensory proteins of Plutella xylostella (Lepidoptera: Plutellidae) were obtained by RACE-PCR. PxylCSP3 and Pxyl-CSP4, with GenBank accession numbers ABM92663 and ABM92664, respectively, were cloned and sequenced. The gene sequences both consisted of three exons and two introns. RT-PCR analysis showed that Pxyl-CSP3 and Pxyl-CSP4 had different expression patterns in the examined developmental stages, but were expressed in all larval stages. Phylogenetic analysis indicated that lepidopteran insects consist of three branches, and Pxyl-CSP3 and Pxyl-CSP4 belong to different branches. The 5'regulatory regions of Pxyl-CSP3 and Pxyl-CSP4 were isolated and analyzed, and the results consist of not only the core promoter sequences (TATA-box), but also several transcriptional elements (BR-C Z4, Hb, Dfd, CF2-II, etc.). This study provides clues to better understanding the various physiological functions of CSPs in P. xylostella and other insects.
authors.affiliation: Key Laboratory of Pesticide and Chemical Biology, Ministry of Education of P.R. China. South China Agricultural University, Guang Zhou 510642, Guangdong, China. 125314689@qq.com
authors.forename: Liang
authors.initials: L
authors.lastname: Gong
authors.forename: Guo-Hua
authors.initials: GH
authors.lastname: Zhong
authors.forename: Mei-Ying
authors.initials: MY
authors.lastname: Hu
authors.forename: Qian
authors.initials: Q
authors.lastname: Luo
authors.forename: Zhen-Zhen
authors.initials: ZZ
authors.lastname: Ren
mesh_terms: D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D016000:Cluster Analysis; D019295:Computational Biology; D017931:DNA Primers / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D019476:Insect Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009036:Moths / Q000235:genetics*; D018034:Olfactory Receptor Neurons / Q000737:chemistry; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D019476:Insect Proteins