title: Anaerobic obligatory xylitol production in Escherichia coli strains devoid of native fermentation pathways.
abstract: Anaerobic glucose oxidation was coupled to xylose reduction in a nonfermentative Escherichia coli strain expressing NADPH-dependent xylose reductase. Xylitol production serves as the primary means of NAD(P)(+) regeneration, as glucose is converted primarily to acetate and CO(2). The membrane-bound transhydrogenase PntAB is required to achieve the maximum theoretical yield of four moles of xylitol per mole of glucose consumed.
authors.affiliation: Department of Chemical Engineering, Pennsylvania State University, University Park, PA 16802, USA.
authors.forename: Olubolaji
authors.initials: O
authors.lastname: Akinterinwa
authors.forename: Patrick C
authors.initials: PC
authors.lastname: Cirino
mesh_terms: D019342:Acetic Acid / Q000378:metabolism; D000449:Aldehyde Reductase / Q000378:metabolism; D000693:Anaerobiosis; D002245:Carbon Dioxide / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000378:metabolism; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D009250:NADP Transhydrogenases / Q000378:metabolism; D010084:Oxidation-Reduction; D014993:Xylitol / Q000096:biosynthesis*
chemical_list: D029968:Escherichia coli Proteins; D002245:Carbon Dioxide; D009249:NADP; D000449:Aldehyde Reductase; D009250:NADP Transhydrogenases; C556049:pntA protein, E coli; C556051:pntB protein, E coli; D005947:Glucose; D019342:Acetic Acid; D014993:Xylitol