title: Production of rhamnolipids in solid-state cultivation using a mixture of sugarcane bagasse and corn bran supplemented with glycerol and soybean oil.
abstract: Rhamnolipid biosurfactants are attracting attention due to their low toxicity, high biodegradability, and good ecological acceptability. However, production in submerged culture is made difficult by severe foaming problems. Solid-state cultivation (SSC) is a promising alternative production method. In the current work, we report the optimization of rhamnolipid production by Pseudomonas aeruginosa UFPEDA 614 on a solid substrate containing sugarcane bagasse and corn bran. The best rhamnolipid production, 45 g/l of impregnating solution used, was obtained with a 50:50 (m/m) mixture of sugarcane bagasse and corn bran supplemented with an impregnating solution containing 6% (v/v) of each of glycerol and soybean oil. This level is comparable with those of previous studies undertaken in solid-state cultivation; the composition of the biosurfactant is similar, but our medium is cheaper. Our work therefore provides a suitable basis for future studies of the development of an SSC-based process for rhamnolipid production.
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Cx.P. 19046 Centro Politécnico, Curitiba 81531-980, Paraná, Brazil.
authors.forename: Doumit
authors.initials: D
authors.lastname: Camilios-Neto
authors.forename: Cryshelen
authors.initials: C
authors.lastname: Bugay
authors.forename: Arquimedes Paixão
authors.initials: AP
authors.lastname: de Santana-Filho
authors.forename: Talita
authors.initials: T
authors.lastname: Joslin
authors.forename: Lauro Mera
authors.initials: LM
authors.lastname: de Souza
authors.forename: Guilherme Lanzi
authors.initials: GL
authors.lastname: Sassaki
authors.forename: David Alexander
authors.initials: DA
authors.lastname: Mitchell
authors.forename: Nadia
authors.initials: N
authors.lastname: Krieger
mesh_terms: D002482:Cellulose / Q000378:metabolism*; D004043:Dietary Fiber; D005285:Fermentation; D005990:Glycerol / Q000378:metabolism*; D006017:Glycolipids / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000378:metabolism*; D031786:Saccharum; D013024:Soybean Oil / Q000378:metabolism*; D003313:Zea mays
chemical_list: D004043:Dietary Fiber; D006017:Glycolipids; C418382:rhamnolipid; D013024:Soybean Oil; D002482:Cellulose; C027433:bagasse; D005990:Glycerol