title: Effects of various agents on DNA fragmentation and telomerase enzyme activities in adenocarcinoma cell lines.
abstract: Natural compounds such as resveratrol, tannic acid, and quercetin may help to treat cancer. Tamoxifen is a non-steroidal anti-estrogen drug widely used in the treatment of patients with estrogen receptor-positive breast cancer. The aim of the study was to compare the effects of these natural compounds and tamoxifen in colon adenocarcinoma (CaCo-2) and breast adenocarcinoma (MCF-7) cell lines, on telomerase enzyme activity, cell viability, number of cells and DNA fragmentation. In this study to determine telomerase enzyme activity was used PCR-ELISA kit. To determine cell viability and number of cells were used tripan blue stain. DNA fragmentation was determined by DNA ladder isolation kit. Tannic acid was more effective than resveratrol, with respect to reduction in telomerase activity, cell viability and cell count in breast adenocarcinoma. Tannic acid and tamoxifen was more effective than resveratrol and quercetin telomerase activity, cell viability and cell count in colon adenocarcinoma. Flavonoids such as resveratrol, tannic acid and quercetin which was studied on, has benefical effects on cancer therapy. These effects such as decreasing telomerase enzyme activity, cell viability and number of cells and inducing DNA fragmentation (apoptosis) must be studied for assist to develop new therapeutic pathways. There should be much more sudies in order to discover resveratrol, tannic acid and quercetin and other potential medicines.
authors.affiliation: Eskişehir Osmangazi Üniversitesi, Tıp Fakültesi, Tıbbi Biyoloji AD, 26480, Eskişehir, Turkey. dcosan@ogu.edu.tr
authors.forename: Didem Turgut
authors.initials: DT
authors.lastname: Cosan
authors.forename: Ahu
authors.initials: A
authors.lastname: Soyocak
authors.forename: Ayse
authors.initials: A
authors.lastname: Basaran
authors.forename: Irfan
authors.initials: I
authors.lastname: Degirmenci
authors.forename: Hasan Veysi
authors.initials: HV
authors.lastname: Gunes
authors.forename: Fezan Mutlu
authors.initials: FM
authors.lastname: Sahin
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism*; D000704:Analysis of Variance; D045744:Cell Line, Tumor; D053938:DNA Fragmentation / Q000187:drug effects*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D011794:Quercetin / Q000494:pharmacology; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology; D013629:Tamoxifen / Q000494:pharmacology; D013634:Tannins / Q000494:pharmacology; D019098:Telomerase / Q000378:metabolism*
chemical_list: D013267:Stilbenes; D013634:Tannins; D013629:Tamoxifen; D011794:Quercetin; D019098:Telomerase; D000077185:Resveratrol