title: Platelet lysate consisting of a natural repair proteome supports human mesenchymal stem cell proliferation and chromosomal stability.
abstract: With favorable regenerative and immunotolerant profiles, patient-derived human mesenchymal stem cells (hMSCs) are increasingly considered in cell therapy. Derived from bone marrow (BM) and standardized with culture in fetal bovine serum (FBS), translation of hMSC-based approaches is impeded by protracted expansion times, risk of xenogenic response, and exposure to zoonoses. Here, human platelet lysate adherent to good manufacturing practices (GMP-hPL) provided a nonzoonotic adjuvant that enhanced the capacity of BM-hMSC to proliferate. The nurturing benefit of GMP-hPL was generalized to hMSC from adipose tissue evaluated as an alternative to bone marrow. Long-term culture in GMP-hPL maintained the multipotency of hMSC, while protecting against clonal chromosomal instability detected in the FBS milieu. Proteomic dissection identified TGF-β, VEGF, PDGF, FGF, and EGF as highly ranked effectors of hPL activity, revealing a paradigm of healing that underlies platelet lysate adjuvancy. Thus, GMP-adherent human platelet lysate accelerates hMSC proliferation with no chromosomal aberrancy, through an innate repair paradigm.
authors.affiliation: Division of Cardiovascular Diseases, Departments of Medicine and Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic, Rochester, MN, USA.
authors.forename: Ruben
authors.initials: R
authors.lastname: Crespo-Diaz
authors.forename: Atta
authors.initials: A
authors.lastname: Behfar
authors.forename: Greg W
authors.initials: GW
authors.lastname: Butler
authors.forename: Douglas J
authors.initials: DJ
authors.lastname: Padley
authors.forename: Michael G
authors.initials: MG
authors.lastname: Sarr
authors.forename: Jozef
authors.initials: J
authors.lastname: Bartunek
authors.forename: Allan B
authors.initials: AB
authors.lastname: Dietz
authors.forename: Andre
authors.initials: A
authors.lastname: Terzic
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D001792:Blood Platelets / Q000166:cytology / Q000502:physiology*; D001854:Bone Marrow Cells / Q000166:cytology; D018929:Cell Culture Techniques; D049109:Cell Proliferation; D002478:Cells, Cultured; D043171:Chromosomal Instability; D006801:Humans; D007621:Karyotyping; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D020543:Proteome; D015398:Signal Transduction
chemical_list: D020543:Proteome