title: Effects of untreated and thermally treated lupin protein on plasma and liver lipids of rats fed a hypercholesterolemic high fat or high carbohydrate diet.
abstract: Lupin protein is capable of reducing plasma lipids in hypercholesterolemic man and animals. Whether lipid-lowering properties of lupin protein will be influenced by thermal treatment or by other nutrients has not been elucidated. In a two-factorial study, rats were fed hypercholesterolemic diets based on high amounts of carbohydrates (HC) or fat (HF), which contained either (20.4% of energy) untreated or thermally treated lupin protein (steam: 120 °C, 30 min) or casein as control protein. Lupin protein lowered plasma lipid concentrations in rats fed the HF diet but not in those fed the HC diet (P<0.05). Among rats fed the HF diet, plasma and VLDL triglyceride concentrations were lower in rats fed thermally treated (-46% and -44%, P<0.05) and untreated lupin protein (-47% and -46%, P<0.05) than in those fed casein; whereas liver triglycerides were reduced only in rats fed untreated lupin protein (P<0.05). Compared to casein, untreated lupin protein had slightly stronger cholesterol-lowering effects in plasma, LDL and HDL (-34%, -37%, -35%; P<0.05) than thermally treated lupin protein (-23%, -29%, -31%, P<0.10). In conclusion, the lipid-lowering effect of lupin protein strongly depends on composition of the basal diet, and thermal treatment is accompanied by a slight reduction of its hypocholesterolemic properties.
authors.affiliation: Institute of Agricultural and Nutritional Sciences, Martin-Luther-University Halle-Wittenberg, Von-Danckelmann-Platz 2, 06120 Halle (Saale), Germany.
authors.forename: Corinna
authors.initials: C
authors.lastname: Brandsch
authors.forename: Diana
authors.initials: D
authors.lastname: Kappis
authors.forename: Kristin
authors.initials: K
authors.lastname: Weisse
authors.forename: Gabriele I
authors.initials: GI
authors.lastname: Stangl
mesh_terms: D000818:Animals; D000924:Anticholesteremic Agents / Q000494:pharmacology*; D002364:Caseins / Q000494:pharmacology; D002791:Cholesterol, Dietary / Q000378:metabolism; D008076:Cholesterol, HDL / Q000097:blood; D008078:Cholesterol, LDL / Q000097:blood; D004040:Dietary Carbohydrates / Q000378:metabolism; D004044:Dietary Proteins / Q000494:pharmacology*; D006937:Hypercholesterolemia / Q000188:drug therapy*; D050356:Lipid Metabolism; D008079:Lipoproteins, VLDL / Q000097:blood; D008099:Liver / Q000378:metabolism; D008297:Male; D010936:Plant Extracts / Q000494:pharmacology; D010940:Plant Proteins / Q000494:pharmacology*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D014280:Triglycerides / Q000097:blood
chemical_list: D000924:Anticholesteremic Agents; D002364:Caseins; D002791:Cholesterol, Dietary; D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D004040:Dietary Carbohydrates; D004044:Dietary Proteins; D008079:Lipoproteins, VLDL; D010936:Plant Extracts; D010940:Plant Proteins; D014280:Triglycerides; C030106:very low density lipoprotein triglyceride