title: A defined glycosaminoglycan-binding substratum for human pluripotent stem cells.
abstract: To exploit the full potential of human pluripotent stem cells for regenerative medicine, developmental biology and drug discovery, defined culture conditions are needed. Media of known composition that maintain human embryonic stem (hES) cells have been developed, but finding chemically defined, robust substrata has proven difficult. We used an array of self-assembled monolayers to identify peptide surfaces that sustain pluripotent stem cell self-renewal. The effective substrates displayed heparin-binding peptides, which can interact with cell-surface glycosaminoglycans and could be used with a defined medium to culture hES cells for more than 3 months. The resulting cells maintained a normal karyotype and had high levels of pluripotency markers. The peptides supported growth of eight pluripotent cell lines on a variety of scaffolds. Our results indicate that synthetic substrates that recognize cell-surface glycans can facilitate the long-term culture of pluripotent stem cells.
authors.affiliation: Cell and Molecular Biology Program, University of Wisconsin-Madison, Madison, Wisconsin, USA.
authors.forename: Joseph R
authors.initials: JR
authors.lastname: Klim
authors.forename: Lingyin
authors.initials: L
authors.lastname: Li
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Wrighton
authors.forename: Marian S
authors.initials: MS
authors.lastname: Piekarczyk
authors.forename: Laura L
authors.initials: LL
authors.lastname: Kiessling
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D002448:Cell Adhesion; D002454:Cell Differentiation; D006025:Glycosaminoglycans / Q000378:metabolism*; D006493:Heparin / Q000378:metabolism*; D006801:Humans; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D010455:Peptides / Q000378:metabolism; D039904:Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D011485:Protein Binding; D011956:Receptors, Cell Surface / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology / Q000502:physiology; D013379:Substrate Specificity
chemical_list: D006025:Glycosaminoglycans; D010446:Peptide Fragments; D010455:Peptides; D011956:Receptors, Cell Surface; D006493:Heparin