title: Isolation of murine adipose-derived stem cells.
abstract: Murine models of obesity or reduced adiposity are a valuable resource for understanding the role of adipocyte dysfunction in metabolic disorders. Primary adipocytes grown in culture and derived from murine adipose tissue are essential for studying the mechanisms underlying adipocyte development and function. Herein, we describe methods for the isolation, expansion, and long-term storage of murine adipose-derived stem cells along with a protocol for inducing adipogenesis in this cell population.
authors.affiliation: Stem Cell Biology Laboratory, Pennington Biomedical Research Center, Louisiana State University System, Baton Rouge, LA, USA.
authors.forename: Gang
authors.initials: G
authors.lastname: Yu
authors.forename: Xiying
authors.initials: X
authors.lastname: Wu
authors.forename: Gail
authors.initials: G
authors.lastname: Kilroy
authors.forename: Yuan-Di C
authors.initials: YD
authors.lastname: Halvorsen
authors.forename: Jeffrey M
authors.initials: JM
authors.lastname: Gimble
authors.forename: Z Elizabeth
authors.initials: ZE
authors.lastname: Floyd
mesh_terms: D017667:Adipocytes / Q000166:cytology; D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D001391:Azo Compounds / Q000378:metabolism; D002454:Cell Differentiation; D002469:Cell Separation / Q000379:methods*; D015925:Cryopreservation; D008055:Lipids / Q000032:analysis; D051379:Mice; D013194:Staining and Labeling; D013234:Stem Cells / Q000166:cytology*; D017154:Stromal Cells / Q000166:cytology
chemical_list: D001391:Azo Compounds; D008055:Lipids; C011049:oil red O