title: Protective effects of brain-derived neurotrophic factor on the noise-damaged cochlear spiral ganglion.
abstract: OBJECTIVE
  To explore the protective effects of brain-derived neurotrophic factor on the noise-damaged cochlear spiral ganglion.
  
  
  METHODS
  Recombinant adenovirus brain-derived neurotrophic factor vector, recombinant adenovirus LacZ and artificial perilymph were prepared. Guinea pigs with audiometric auditory brainstem response thresholds of more than 75 dB SPL, measured seven days after four hours of noise exposure at 135 dB SPL, were divided into three groups. Adenovirus brain-derived neurotrophic factor vector, adenovirus LacZ and perilymph were infused into the cochleae of the three groups, variously. Eight weeks later, the cochleae were stained immunohistochemically and the spiral ganglion cells counted.
  
  
  RESULTS
  The auditory brainstem response threshold recorded before and seven days after noise exposure did not differ significantly between the three groups. However, eight weeks after cochlear perfusion, the group receiving brain-derived neurotrophic factor had a significantly decreased auditory brainstem response threshold and increased spiral ganglion cell count, compared with the adenovirus LacZ and perilymph groups.
  
  
  CONCLUSIONS
  When administered via cochlear infusion following noise damage, brain-derived neurotrophic factor appears to improve the auditory threshold, and to have a protective effect on the spiral ganglion cells.
authors.affiliation: Institute of Otolaryngology-Head and Neck Surgery, Chinese People's Liberation Army General Hospital, Beijing, China. gaochunyan1954@163.com
authors.forename: S-Q
authors.initials: SQ
authors.lastname: Zhai
authors.forename: W
authors.initials: W
authors.lastname: Guo
authors.forename: Y-Y
authors.initials: YY
authors.lastname: Hu
authors.forename: N
authors.initials: N
authors.lastname: Yu
authors.forename: Q
authors.initials: Q
authors.lastname: Chen
authors.forename: J-Z
authors.initials: JZ
authors.lastname: Wang
authors.forename: M
authors.initials: M
authors.lastname: Fan
authors.forename: W-Y
authors.initials: WY
authors.lastname: Yang
mesh_terms: D000256:Adenoviridae; D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000008:administration & dosage / Q000096:biosynthesis / Q000494:pharmacology*; D002452:Cell Count; D002470:Cell Survival / Q000187:drug effects; D003051:Cochlea / Q000187:drug effects* / Q000378:metabolism; D004195:Disease Models, Animal; D016057:Evoked Potentials, Auditory, Brain Stem / Q000502:physiology; D015316:Genetic Therapy; D005822:Genetic Vectors; D006168:Guinea Pigs; D006317:Hearing Loss, Noise-Induced / Q000503:physiopathology*; D007763:Lac Operon; D009622:Noise / Q000009:adverse effects; D010498:Perilymph; D013136:Spiral Ganglion / Q000187:drug effects* / Q000473:pathology
chemical_list: D019208:Brain-Derived Neurotrophic Factor