title: In vivo optical imaging of revascularization after brain trauma in mice.
abstract: Revascularization following brain trauma is crucial to the repair process. We used optical micro-angiography (OMAG) to study endogenous revascularization in living mice following brain injury. OMAG is a volumetric optical imaging method capable of in vivo mapping of localized blood perfusion within the scanned tissue beds down to capillary level imaging resolution. We demonstrated that OMAG can differentiate revascularization progression between traumatized mice with and without soluble epoxide hydrolase (sEH) gene deletion. The time course of revascularization was determined from serial imaging of the traumatic region in the same mice over a one-month period of rehabilitation. Restoration of blood volume at the lesion site was more pronounced in sEH knockout mice than in wild-type mice as determined by OMAG. These OMAG measurements were confirmed by histology and showed that the sEH knockout effect may be involved in enhancing revascularization. The correlation of OMAG with histology also suggests that OMAG is a useful imaging tool for real-time in vivo monitoring of post-traumatic revascularization and for evaluating agents that inhibit or promote endogenous revascularization during the recovery process in small rodents.
authors.affiliation: Department of Biomedical Engineering, School of Medicine, Oregon Health & Science University, 3303 SW Bond Avenue, Portland, OR 97239, USA.
authors.forename: Yali
authors.initials: Y
authors.lastname: Jia
authors.forename: Marjorie R
authors.initials: MR
authors.lastname: Grafe
authors.forename: Andras
authors.initials: A
authors.lastname: Gruber
authors.forename: Nabil J
authors.initials: NJ
authors.lastname: Alkayed
authors.forename: Ruikang K
authors.initials: RK
authors.lastname: Wang
mesh_terms: D000818:Animals; D018952:Antigens, CD34 / Q000378:metabolism; D001930:Brain Injuries / Q000473:pathology; D002533:Cerebral Angiography / Q000379:methods*; D002540:Cerebral Cortex / Q000098:blood supply* / Q000293:injuries* / Q000473:pathology / Q000502:physiology; D002560:Cerebrovascular Circulation / Q000502:physiology; D004851:Epoxide Hydrolases / Q000235:genetics; D021621:Imaging, Three-Dimensional / Q000379:methods; D017078:Laser-Doppler Flowmetry; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D055806:Microvessels / Q000473:pathology / Q000502:physiology; D018919:Neovascularization, Physiologic / Q000502:physiology*
chemical_list: D018952:Antigens, CD34; D004851:Epoxide Hydrolases