title: Identification and characterization of integron-associated antibiotic resistant Laribacter hongkongensis isolated from aquatic products in China.
abstract: Laribacter hongkongensis is a recently discovered bacterium associated with gastroenteritis. In this study, a total of 199 isolates of this species obtained from aquatic products (n=462) in Guangzhou City, China, were examined for their susceptibility to 19 antimicrobial agents and the presence of antimicrobial resistance integrons. The genetic relatedness of the isolates with integrons was also evaluated. A PCR-based method was used to screen integrons and found that 13 (6.5%) of the isolates harbored class 1 integrons. The antimicrobial resistance rates of integron-positive isolates were significantly higher than integron-negative ones for cefepime, ciprofloxacin, gentamicin, tetracycline, trimethoprim-sulfamethoxazole and rifampicin. Genetic sequence analysis revealed that these integrons contained various antimicrobial-resistance genes (dfrA1, dfrA14, dfrA17, dfrA32, aadA1, aadA2, aadA5, cmlA5, arr2, ereA and orfC) organized into different gene cassettes arrangements including a novel array of dfrA14-arr2-cmlA5. Pulsed-field gel electrophoresis (PFGE) yielded 13 different patterns among 13 integron-positive isolates, which could be grouped into four clusters. These indicate the dispersal of multi-resistant integrons among different molecular types. To the best of our knowledge, this is the first report showing distribution and characterization of class 1 integrons among L. hongkongensis isolates.
authors.affiliation: Department of Epidemiology, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515, China.
authors.forename: Jia-li
authors.initials: JL
authors.lastname: Feng
authors.forename: He
authors.initials: H
authors.lastname: Yan
authors.forename: Nityananda
authors.initials: N
authors.lastname: Chowdhury
authors.forename: Sucharit Basu
authors.initials: SB
authors.lastname: Neogi
authors.forename: Shinji
authors.initials: S
authors.lastname: Yamasaki
authors.forename: Lei
authors.initials: L
authors.lastname: Shi
authors.forename: Jing
authors.initials: J
authors.lastname: Hu
authors.forename: Qing
authors.initials: Q
authors.lastname: Chen
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001001:Anura / Q000382:microbiology; D002347:Carps / Q000382:microbiology; D002681:China; D016000:Cluster Analysis; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field; D005516:Food Microbiology; D032023:Integrons / Q000235:genetics*; D009346:Neisseriaceae / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D000900:Anti-Bacterial Agents