title: Progesterone-induced reorganisation of NOX-2 components in membrane rafts is critical for sperm functioning in Capra hircus.
abstract: Spermatozoa produce increasing levels of reactive oxygen species (ROS) during development. We examined the functional organisation of NOX-2 subunits in developing spermatozoa and its involvement in ROS generation. We found NOX-2 components associated predominantly with caveolin-rich microdomains of sperm head and mid piece membranes. Capacitation in vitro enriched NOX-2 components within the caveolin-positive sperm membrane rafts. Progesterone, but not 17β-oestradiol, brought about loss of caveolin and extensive reorganisations in NOX-2 distribution on sperm head microdomains and accompanying activation in the levels of superoxide. The sensitivity of superoxide production by spermatozoa to methyl-β-cyclodextrin confirms that NOX-2 function in spermatozoa is tightly dependent on raft organisation. We conclude that progesterone-induced reorganisation of NOX-2 components in sperm membrane rafts is critical for sperm functioning.
authors.affiliation: Division of Molecular Reproduction, Rajiv Gandhi Centre for Biotechnology, Thycaud Post, Thiruvananthapuram, Kerala, India.
authors.forename: M
authors.initials: M
authors.lastname: Shoeb
authors.forename: M
authors.initials: M
authors.lastname: Laloraya
authors.forename: P G
authors.initials: PG
authors.lastname: Kumar
mesh_terms: D020101:Acrosome Reaction / Q000187:drug effects; D000818:Animals; D051242:Caveolin 1 / Q000378:metabolism; D006041:Goats; D008297:Male; D008562:Membrane Glycoproteins / Q000378:metabolism*; D021962:Membrane Microdomains / Q000187:drug effects* / Q000502:physiology; D019255:NADPH Oxidases / Q000378:metabolism*; D011374:Progesterone / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013075:Sperm Capacitation / Q000502:physiology; D013094:Spermatozoa / Q000187:drug effects / Q000502:physiology*; D047392:beta-Cyclodextrins / Q000494:pharmacology
chemical_list: D051242:Caveolin 1; D008562:Membrane Glycoproteins; D017382:Reactive Oxygen Species; D047392:beta-Cyclodextrins; C108732:methyl-beta-cyclodextrin; D011374:Progesterone; D019255:NADPH Oxidases