title: Multipotent PDGFRβ-expressing cells in the circulation of stroke patients.
abstract: Tissue pericytes respond to injury, and support vascular and tissue regeneration. The presence of pericytes in the circulation may provide an attractive framework for tissue regeneration. Here, we detected multipotent pericyte-like cells in the circulating blood and determined its profiles during cerebral ischemia. Pericyte-like cells were isolated from the peripheral blood of acute stroke patients or asymptomatic individuals with vascular risk factors by fluorescence or magnetic activated cell sorting with anti-PDGF receptor-beta (PDGFRβ) antibody. The morphologic and molecular features of circulating PDGFRβ(+) cells were compared with tissue pericytes, and the associations with respect to quantity in the blood, culture outcome, and patient characteristics were analyzed. We found an increase in circulating PDGFRβ(+) cells in acute stroke patients compared to controls and a correlation with neurologic impairment. The isolated PDGFRβ(+) cells expressed mesenchymal stem cell markers, proliferated, and were multipotent under permissive culture conditions. The multipotent nature of these cells was comparable to fat-derived PDGFRβ(+) cells. These cells could be obtained by pharmacologic stimulation using bone marrow mobilizer. Circulating PDGFRβ(+) cells will be useful for future research involving endogenous recovery or autologous cell-based therapy.
authors.affiliation: Department of Neurology, Stroke & Stem Cell Laboratory, Clinical Research Institute, Seoul National University Hospital, Seoul, South Korea.
authors.forename: Keun-Hwa
authors.initials: KH
authors.lastname: Jung
authors.forename: Kon
authors.initials: K
authors.lastname: Chu
authors.forename: Soon-Tae
authors.initials: ST
authors.lastname: Lee
authors.forename: Jae-Jun
authors.initials: JJ
authors.lastname: Bahn
authors.forename: Daejong
authors.initials: D
authors.lastname: Jeon
authors.forename: Jin-Hee
authors.initials: JH
authors.lastname: Kim
authors.forename: Soyun
authors.initials: S
authors.lastname: Kim
authors.forename: Chong-Hyun
authors.initials: CH
authors.lastname: Won
authors.forename: Manho
authors.initials: M
authors.lastname: Kim
authors.forename: Sang Kun
authors.initials: SK
authors.lastname: Lee
authors.forename: Jae-Kyu
authors.initials: JK
authors.lastname: Roh
mesh_terms: D000208:Acute Disease; D000368:Aged; D015415:Biomarkers / Q000097:blood; D018929:Cell Culture Techniques; D002469:Cell Separation / Q000379:methods*; D005260:Female; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000378:metabolism* / Q000473:pathology; D008875:Middle Aged; D039902:Multipotent Stem Cells / Q000378:metabolism* / Q000473:pathology; D009416:Nerve Regeneration / Q000235:genetics; D020286:Pericytes / Q000378:metabolism* / Q000473:pathology; D020797:Receptor, Platelet-Derived Growth Factor beta / Q000096:biosynthesis* / Q000097:blood* / Q000235:genetics; D020521:Stroke / Q000097:blood / Q000378:metabolism* / Q000473:pathology*
chemical_list: D015415:Biomarkers; D020797:Receptor, Platelet-Derived Growth Factor beta