title: Senescence in cells in aseptic loosening after total hip replacement.
abstract: Particle-induced osteolysis is a major cause of aseptic loosening after total joint replacement. The purpose of the current study was to evaluate cellular senescence of macrophages and giant cells in patients with aseptic hip loosening by determination of SA-β-Gal (SA-β-galactosidase), a reliable and frequently used indicator of cellular senescence. The level of senescence in capsule and interface membranes was significantly higher in patients with aseptic loosening in comparison to specimens from patients without aseptic loosening. Using Spearman's rank correlation, we found that the expression of SA-β-Gal in giant cells (p=0.002) and macrophages (p=0.050) in the interface membranes correlates significantly with the degree of polyethylene debris. We speculate that the induction of DNA damage by wear particles is responsible for premature senescence. Consequently, we conclude that the form of senescence observed in this study is a "stress-induced senescence".
authors.affiliation: Department of Orthopaedics, University of Duisburg-Essen, and Department of Pathology, University Hospital of Essen, Pattbergstrasse 1-3, 45239 Essen, Germany. stefan.landgraeber@uni-due.de
authors.forename: Stefan
authors.initials: S
authors.lastname: Landgraeber
authors.forename: Ulrich
authors.initials: U
authors.lastname: Quint
authors.forename: Tim
authors.initials: T
authors.lastname: Classen
authors.forename: Martin
authors.initials: M
authors.lastname: Totsch
mesh_terms: D000368:Aged; D019644:Arthroplasty, Replacement, Hip; D016922:Cellular Senescence; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D001616:beta-Galactosidase