title: Study of the binding equilibrium between Zn(II) and HSA by capillary electrophoresis-inductively coupled plasma optical emission spectrometry.
abstract: A new method has been developed for following the interaction between zinc ion and human serum albumin (HSA) by capillary electrophoresis-inductively coupled plasma optical emission spectrometry. Under optimized experimental conditions, the detection limit (3σ) for free Zn(2+) ion was found to be 1.34 μM by running 11 replicates of the reagent blank. The RSD was less than 3% and the recovery was more than 98.13%. The linear range of zinc ion concentration was between 5.1 μM and 0.3M. The measured Zn(II)-HSA combination values of n(1) and K(1) for primary binding of Zn(2+) to HSA were 1.09 and 2.29×10(5) L mol(-1), respectively. The measured values of n(2) and K(2) for the non-specific binding of Zn(2+) to HSA were 8.96 and 6.65×10(3) L mol(-1), respectively. This new method allows rapid analysis of a small amount of sample, simple operation, while avoiding long periods of dialysis and eliminating interference from other metal ions. This method provides a reliable and convenient new way for studying interactions between metal ions and biomolecules.
authors.affiliation: Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China), College of Chemistry and Chemical Engineering, Guangxi Normal University, Guilin 541004, China. dengby16@163.com
authors.forename: Biyang
authors.initials: B
authors.lastname: Deng
authors.forename: Yingzi
authors.initials: Y
authors.lastname: Wang
authors.forename: Pingchuan
authors.initials: P
authors.lastname: Zhu
authors.forename: Xiangshu
authors.initials: X
authors.lastname: Xu
authors.forename: Xi
authors.initials: X
authors.lastname: Ning
mesh_terms: D001665:Binding Sites; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D007700:Kinetics; D013058:Mass Spectrometry / Q000379:methods*; D011485:Protein Binding; D012709:Serum Albumin / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism*; D015032:Zinc / Q000097:blood* / Q000737:chemistry / Q000378:metabolism*
chemical_list: D012709:Serum Albumin; D015032:Zinc