title: Coastal bacterioplankton community diversity along a latitudinal gradient in Latin America by means of V6 tag pyrosequencing.
abstract: The bacterioplankton diversity of coastal waters along a latitudinal gradient between Puerto Rico and Argentina was analyzed using a total of 134,197 high-quality sequences from the V6 hypervariable region of the small-subunit ribosomal RNA gene (16S rRNA) (mean length of 60 nt). Most of the OTUs were identified into Proteobacteria, Bacteriodetes, Cyanobacteria, and Actinobacteria, corresponding to approx. 80% of the total number of sequences. The number of OTUs corresponding to species varied between 937 and 1946 in the seven locations. Proteobacteria appeared at high frequency in the seven locations. An enrichment of Cyanobacteria was observed in Puerto Rico, whereas an enrichment of Bacteroidetes was detected in the Argentinian shelf and Uruguayan coastal lagoons. The highest number of sequences of Actinobacteria and Acidobacteria were obtained in the Amazon estuary mouth. The rarefaction curves and Good coverage estimator for species diversity suggested a significant coverage, with values ranging between 92 and 97% for Good coverage. Conserved taxa corresponded to aprox. 52% of all sequences. This study suggests that human-contaminated environments may influence bacterioplankton diversity.
authors.affiliation: Departments of Genetics, Institute of Biology, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil. fabiano.thompson@biologia.ufrj.br
authors.forename: Fabiano L
authors.initials: FL
authors.lastname: Thompson
authors.forename: Thiago
authors.initials: T
authors.lastname: Bruce
authors.forename: Alessandra
authors.initials: A
authors.lastname: Gonzalez
authors.forename: Alexander
authors.initials: A
authors.lastname: Cardoso
authors.forename: Maysa
authors.initials: M
authors.lastname: Clementino
authors.forename: Marcela
authors.initials: M
authors.lastname: Costagliola
authors.forename: Constanza
authors.initials: C
authors.lastname: Hozbor
authors.forename: Ernesto
authors.initials: E
authors.lastname: Otero
authors.forename: Claudia
authors.initials: C
authors.lastname: Piccini
authors.forename: Silvia
authors.initials: S
authors.lastname: Peressutti
authors.forename: Robert
authors.initials: R
authors.lastname: Schmieder
authors.forename: Robert
authors.initials: R
authors.lastname: Edwards
authors.forename: Mathew
authors.initials: M
authors.lastname: Smith
authors.forename: Luis Roberto
authors.initials: LR
authors.lastname: Takiyama
authors.forename: Ricardo
authors.initials: R
authors.lastname: Vieira
authors.forename: Rodolfo
authors.initials: R
authors.lastname: Paranhos
authors.forename: Luis Felipe
authors.initials: LF
authors.lastname: Artigas
mesh_terms: D039903:Actinobacteria / Q000235:genetics / Q000302:isolation & purification; D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D041963:Bacteroidetes / Q000235:genetics / Q000302:isolation & purification; D044822:Biodiversity; D000458:Cyanobacteria / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D007843:Latin America; D010933:Plankton / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D020560:Proteobacteria / Q000235:genetics / Q000302:isolation & purification; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014871:Water Microbiology
chemical_list: D012336:RNA, Ribosomal, 16S