title: Identification of moderately halophilic bacteria from Thai fermented fish ( pla-ra ) and proposal of Virgibacillus siamensis sp. nov.
abstract: Forty-one isolates of moderately halophilic bacteria were isolated from fermented fish (pla-ra) in Thailand. On the basis of their phenotypic and chemotaxonomic characteristics, DNA-DNA relatedness and 16S rRNA gene sequences analyses, they were divided into six groups. The isolates in Group I to V were Gram-positive rod-shaped bacteria. They contained meso-diaminopimelic acid in the cell-wall peptidoglycan and menaquinone with seven isoprene units (MK-7). An isolate in Group VI was a Gram-negative rod-shaped bacterium. The DNA G+C contents of tested strains ranged from 36.5-63 mol%. Ten strains (Group I) were identified as Virgibacillus dokdonensis, 13 isolates (Group II) as V. halodenitrificans, 14 isolates (Group III) as V. marismortui, 1 isolate (Group IV) as Virgibacillus sp., 2 isolates (Group V) as Bacillus vietnamnensis, and 1 isolate (Group VI) as Chromohalobacter salexigens. Isolate MS3-4 in Group IV was closely related to V. carmonensis KCTC 3819(T) (95.9%). This strain contained anteiso-C(15:0) (55.8%) and anteiso-C(17:0) (17.7%) as major cellular fatty acids and had phosphatidylglycerol, diphosphatidylglycerol and an unidentified glycolipid as polar lipids. The DNA G+C content of MS3-4 was 38.0 mol%. The strain from Group IV is proposed as Virgibacillus siamensis sp. nov. and MS3-4(T) is the type strain (JCM 15395(T) =PCU 312(T) =TISTR 1957(T)).
authors.affiliation: Department of Biochemistry and Microbiology, Chulalongkorn University, Bangkok, Thailand. Somboon.T@chula.ac.th
authors.forename: Somboon
authors.initials: S
authors.lastname: Tanasupawat
authors.forename: Nitcha
authors.initials: N
authors.lastname: Chamroensaksri
authors.forename: Takuji
authors.initials: T
authors.lastname: Kudo
authors.forename: Takashi
authors.initials: T
authors.lastname: Itoh
mesh_terms: D000818:Animals; D005285:Fermentation; D005396:Fish Products / Q000382:microbiology*; D005399:Fishes / Q000382:microbiology*; D005516:Food Microbiology; D010802:Phylogeny; D013785:Thailand; D058679:Virgibacillus / Q000145:classification* / Q000302:isolation & purification