title: Reducing acyl migration during purification of 2-arachidonoylglycerol from biological samples before gas chromatography mass spectrometry analysis.
abstract: Endocannabinoid 2-arachidonoylglycerol (2-AG) regulates several important physiological processes in the brain. 2-AG is commonly quantified by gas chromatography mass spectrometry after an initial purification step. The most precise and rapid purification utilizes C(18) solid-phase extraction, but quantification problems can arise with acyl migration from 2-AG to 1-arachidonoylglycerol. We found that extraction with methanol promoted this migration, but acetone and diethyl ether (Et(2)O) did not. Acetone and Et(2)O were used to develop a purification method for the direct determination of 2-AG.
authors.affiliation: Department of Neuropharmacology, Faculty of Pharmaceutical Sciences, Fukuoka University, 8-19-1 Nanakuma, Fukuoka 814–0180, Japan.
authors.forename: Sei
authors.initials: S
authors.lastname: Higuchi
authors.forename: Keiichi
authors.initials: K
authors.lastname: Irie
authors.forename: Takafumi
authors.initials: T
authors.lastname: Nakano
authors.forename: Yuya
authors.initials: Y
authors.lastname: Sakamoto
authors.forename: Yoshiharu
authors.initials: Y
authors.lastname: Akitake
authors.forename: Maiko
authors.initials: M
authors.lastname: Araki
authors.forename: Makiko
authors.initials: M
authors.lastname: Ohji
authors.forename: Riyo
authors.initials: R
authors.lastname: Furuta
authors.forename: Mai
authors.initials: M
authors.lastname: Katsuki
authors.forename: Ryuji
authors.initials: R
authors.lastname: Yamaguchi
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Matsuyama
authors.forename: Kenji
authors.initials: K
authors.lastname: Mishima
authors.forename: Kenichi
authors.initials: K
authors.lastname: Mishima
authors.forename: Katsunori
authors.initials: K
authors.lastname: Iwasaki
authors.forename: Michihiro
authors.initials: M
authors.lastname: Fujiwara
mesh_terms: D000096:Acetone / Q000737:chemistry; D000818:Animals; D001095:Arachidonic Acids / Q000032:analysis*; D001921:Brain; D063388:Endocannabinoids; D004986:Ether / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry; D005989:Glycerides / Q000032:analysis*; D008297:Male; D000432:Methanol / Q000737:chemistry; D051379:Mice; D008813:Mice, Inbred ICR; D052616:Solid Phase Extraction
chemical_list: D001095:Arachidonic Acids; D063388:Endocannabinoids; D005989:Glycerides; D004986:Ether; D000096:Acetone; C094503:glyceryl 2-arachidonate; D000432:Methanol