title: Cross-reactivity of antibodies in some commercial deoxynivalenol test kits against some fusariotoxins.
abstract: Cross-reactivity of antibodies in AGRAQUANT, DON EIA, VERATOX, ROSA LF-DONQ, and MYCONTROLDON designed for deoxynivalenol (DON) determination in food and feedstuffs was evaluated against nivalenol, 3-acetylDON, 15-acetylDON, de-epoxy metabolite 1 of DON, DON-3β-glucoside, T2-toxin, HT2-toxin, fusarenone X, diacetoxyscirpenol, verrucarol, and zearalenone. Cross-reactivity measurements were run in water using the 50% reduction of absorbance of the blank for ELISA kits or through direct DON determination upon using the standards of mycotoxins via ROSA LF-DONQ or MYCONTROLDON. For the tested toxin concentrations, all DON kits have low cross-reactivity toward diacetoxyscirpenol, T2-toxin, HT2-toxin, verrucarol, and zearalenone and moderate cross-reactivity toward 15-AcetylDON and fusarenone X. AGRAQUANT, DON EIA, and VERATOX kits showed high cross-reactivity in various ranking orders against DON-3-Glc, DOM-1, and 3AcDON. DON EIA showed also high cross-reactivity against nivalenol and fusarenone X. These mycotoxins could coexist in food or feedstuffs, and analytical results can be wrongly interpreted. Cross-reactivity does not allow checking the compliance with the legal norms, but it does allow an overall risk assessment for the consumers. Updating regularly the cross-reactivity evaluation of the produced batches is recommended for 3-acetylDON, nivalenol, DON-3-Glc, de-epoxy metabolite 1, and fusarenone X.
authors.affiliation: Veterinary and Agrochemical Research Centre (CODA-CERVA), Operational Directorate of Chemical Safety of the Food Chain, Unit of Toxins and Natural Components, Leuvensesteenweg 17, 3080 Tervuren, Belgium. emtan@var.fgov.be
authors.forename: Emmanuel K
authors.initials: EK
authors.lastname: Tangni
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Motte
authors.forename: Alfons
authors.initials: A
authors.lastname: Callebaut
authors.forename: Luc
authors.initials: L
authors.lastname: Pussemier
mesh_terms: D000908:Antibodies, Fungal / Q000032:analysis / Q000276:immunology*; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation; D005670:Fusarium / Q000737:chemistry* / Q000276:immunology; D009183:Mycotoxins / Q000032:analysis* / Q000276:immunology; D011933:Reagent Kits, Diagnostic; D014255:Trichothecenes / Q000032:analysis* / Q000276:immunology
chemical_list: D000908:Antibodies, Fungal; D009183:Mycotoxins; D011933:Reagent Kits, Diagnostic; D014255:Trichothecenes; C007262:deoxynivalenol