title: The correlation between the endometrial integrins and osteopontin expression with pinopodes development in ovariectomized mice in response to exogenous steroids hormones.
abstract: The ovariectomized animals are good models to evaluate the effect of different steroid hormone treatments on implantation events and the pattern of integrin expression. Therefore, this study was performed to compare the expression of integrins and osteopontin (OPN) in correlation with pinopode development in ovariectomized mice endometrium which was subjected to steroid hormones.
  
  
  
  Ovariectomized mice were subjected to estrogen, progesterone and estrogen-progesterone hormones. Their uterine horns were evaluated for integrin expression by immunohistochemistry and real-time RT-PCR and for pinopode development by transmission and scanning electron microscopic studies.
  
  
  
  No immunostaining for integrin and OPN molecules were detected in the endometrium of non-ovariectomized mice except in metestrus phase. The α4 and β1 integrin genes were expressed in all phases of estrous cycle. In ovariectomized mice, no reaction was detected in the endometrium of control, sham and estrogen-treated groups, but in both progesterone-treated groups, all examined genes were expressed. There was not any correlation between pinopodes and integrin expression in ovariectomized mice.
  
  
  
  The progesterone is more effective on endometrial integrin expression than estrogen and differences in the expression pattern of integrins reflect their important and different roles in embryo implantation. The pinopodes may have minor effect in mice implantation or have some delay in their expressions in ovariectomized mice which were subjected to exogenous hormones.
authors.affiliation: Dept. of Anatomy, Tarbiat Modares University, Tehran, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Peyghambari
authors.affiliation: Dept. of Anatomy, Tarbiat Modares University, Tehran, Iran.
authors.forename: Mojdeh
authors.initials: M
authors.lastname: Salehnia
authors.affiliation: Dept. of Biotechnology, Tarbiat Modares University, Tehran, Iran.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Forouzandeh Moghadam
authors.affiliation: Dept. of Anatomy, Tarbiat Modares University, Tehran, Iran.
authors.forename: Mojtaba
authors.initials: M
authors.lastname: Rezazadeh Valujerdi
authors.affiliation: Dept. of Biostatistics, Tarbiat Modares University, Tehran, Iran.
authors.forename: Ebrahim
authors.initials: E
authors.lastname: Hajizadeh
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism*; D004717:Endometrium / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism* / Q000648:ultrastructure; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D004967:Estrogens / Q000494:pharmacology; D030762:Estrous Cycle / Q000187:drug effects / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D006728:Hormones / Q000494:pharmacology*; D007150:Immunohistochemistry; D016023:Integrins / Q000235:genetics / Q000378:metabolism*; D008024:Ligands; D051379:Mice; D053495:Osteopontin / Q000235:genetics / Q000378:metabolism*; D010052:Ovariectomy; D011374:Progesterone / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013256:Steroids / Q000494:pharmacology*
chemical_list: D015415:Biomarkers; D004967:Estrogens; D006728:Hormones; D016023:Integrins; D008024:Ligands; D013256:Steroids; D053495:Osteopontin; D011374:Progesterone