title: Oxidative stress induced by gibberellic acid in bone of suckling rats.
abstract: The present study investigates the bone maturity of suckling rats whose mothers were treated with gibberellic acid (GA(3)). Female Wistar rats were divided into two groups: group I that served as controls and group II that received orally GA(3) (200 ppm) from the 14th day of pregnancy until day 14 after delivery. In the GA(3) group, an increase in body and femur weights as well as in femur length of pups was noted when compared to controls. Lipid peroxidation was demonstrated by high femur malondialdehyde levels, while superoxide dismutase, catalase, glutathione peroxidase activities, glutathione and vitamin C levels in femur decreased. GA(3) caused a decrease in calcium and phosphorus levels in bone. The calcium concentration in plasma increased and the phosphorus concentration decreased, while urinary levels of calcium decreased and those of phosphate increased. Moreover, plasma total tartrate-resistant acid phosphatase and total alkaline phosphatase increased. Bone disorders were confirmed by femur histological changes.
authors.affiliation: University of Sfax, Animal Physiology Laboratory, Life Sciences Department, Sfax Faculty of Science, BP 1171, 3000 Sfax, Tunisia.
authors.forename: Afef
authors.initials: A
authors.lastname: Troudi
authors.forename: Ibtissem
authors.initials: I
authors.lastname: Ben Amara
authors.forename: Nejla
authors.initials: N
authors.lastname: Soudani
authors.forename: Hanen
authors.initials: H
authors.lastname: Bouaziz
authors.forename: Tahia
authors.initials: T
authors.lastname: Boudawara
authors.forename: Najiba
authors.initials: N
authors.lastname: Zeghal
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D000833:Animals, Suckling; D001205:Ascorbic Acid / Q000378:metabolism; D001835:Body Weight / Q000187:drug effects; D002118:Calcium / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D004785:Environmental Pollutants / Q000633:toxicity*; D005260:Female; D005269:Femur / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D005875:Gibberellins / Q000633:toxicity*; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D007527:Isoenzymes / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D010758:Phosphorus / Q000378:metabolism; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism; D000071681:Tartrate-Resistant Acid Phosphatase
chemical_list: D004785:Environmental Pollutants; D005875:Gibberellins; D007527:Isoenzymes; D010758:Phosphorus; D008315:Malondialdehyde; C007842:gibberellic acid; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase; D005978:Glutathione; D001205:Ascorbic Acid; D002118:Calcium