title: Cloning and expression of stearoyl-CoA desaturase 1 (SCD-1) in the liver of the Sichuan white goose and landes goose responding to overfeeding.
abstract: The EST sequence of goose (Anser cygnoides) Stearoyl-CoA desaturase 1(SCD-1) was obtained from a subtractive cDNA library. To further investigate the role of SCD-1 in lipid metabolism in geese, 5'-RACE and 3'-RACE were carried out in this study to obtain the complete cDNA sequence of goose SCD-1, which contained a 29-bp 5' UTR, a 1074-bp open reading frame (ORF) encoding 357 amino acids, and a 125-bp 3' UTR. The expression of SCD-1 was measured in several tissues, and the effects of overfeeding on the expression of SCD-1 were studied. The results of real time RT-PCR demonstrated that, compared to the brain, goose SCD-1 mRNA was more abundant in the liver. Overfeeding markedly increased the mRNA expression of SCD-1 in the liver of Sichuan White and Landes geese, and gene expression was markedly higher in the Sichuan White goose than in the landes goose. The mRNA abundance of SCD-1 in the liver had significant positive correlations with triacylglycerol (TG) content in liver lipids and in the levels of plasma insulin and very low-density lipoproteins (VLDL) levels in Sichuan white geese. However, the mRNA abundance of SCD-1 in the livers of Landes geese had only significant positive correlations with the TG content in liver lipids. In conclusion, SCD-1 is not only critical for hepatic steatosis in geese but is also important for the difference in lipid deposition in the livers of the two breeds.
authors.affiliation: Key Lab of Animal Genetic Resources, College of Animal Science and Technology, Sichuan Agricultural University, Ya'an, Sichuan 625014, People's Republic of China.
authors.forename: Zhi-Xiong
authors.initials: ZX
authors.lastname: Pan
authors.forename: Chun-Chun
authors.initials: CC
authors.lastname: Han
authors.forename: Ji-Wen
authors.initials: JW
authors.lastname: Wang
authors.forename: Liang
authors.initials: L
authors.lastname: Li
authors.forename: Hui
authors.initials: H
authors.lastname: Tang
authors.forename: Jia
authors.initials: J
authors.lastname: Lv
authors.forename: Lizhi
authors.initials: L
authors.lastname: Lu
authors.forename: Feng
authors.initials: F
authors.lastname: Xu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004435:Eating; D020224:Expressed Sequence Tags; D005234:Fatty Liver / Q000235:genetics / Q000378:metabolism; D005777:Geese / Q000033:anatomy & histology / Q000235:genetics / Q000378:metabolism*; D022781:Hepatocytes / Q000166:cytology / Q000378:metabolism; D050356:Lipid Metabolism / Q000235:genetics; D008079:Lipoproteins, VLDL / Q000235:genetics / Q000378:metabolism; D008099:Liver / Q000201:enzymology*; D008297:Male; D008969:Molecular Sequence Data; D044343:Overnutrition; D010802:Phylogeny; D016415:Sequence Alignment; D013230:Stearoyl-CoA Desaturase / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D014280:Triglycerides / Q000235:genetics / Q000378:metabolism
chemical_list: D008079:Lipoproteins, VLDL; D014280:Triglycerides; D013230:Stearoyl-CoA Desaturase