title: Factor V Leiden and FII 20210 testing in thromboembolic disorders.
abstract: Factor V Leiden and prothrombin (F2) c.20210G>A mutation detection are very important in order to define the increased relative risk for venous thromboembolism in selected patients. Use of DNA-based methods to detect both mutations has become widely available in clinical diagnostic laboratories, including fluorescence-based quantitative real-time PCR (qPCR). The latter is a rapid, simple, robust and reliable method to identify genotypes of interest. There are several chemistries used for qPCR; this article describes their principles and applicability for Factor V Leiden and prothrombin (F2) c.20210G>A mutation detection.
authors.affiliation: Department of Haematology, Division of Internal Medicine, University Medical Centre Ljubljana, Ljubljana, Slovenia. tadej.pajic@kclj.si
authors.forename: Tadej
authors.initials: T
authors.lastname: Pajič
mesh_terms: D020016:Activated Protein C Resistance / Q000235:genetics; D019963:Clinical Chemistry Tests; D005165:Factor V / Q000235:genetics*; D006801:Humans; D006868:Hydrolysis; D009154:Mutation; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D011516:Prothrombin / Q000235:genetics*; D013923:Thromboembolism / Q000235:genetics*
chemical_list: C095381:factor V Leiden; D005165:Factor V; D011516:Prothrombin