title: Dynamic changes of platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31) on pulmonary injury induced by ischemia-reperfusion in rats.
abstract: OBJECTIVE
  To evaluate the dynamic changes of PECAM-1/CD31 on pulmonary injury induced by ischemia-reperfusion (IR) in rats.
  
  
  METHODS
  An isolated lung ischemia in rats was performed for 60 min, followed separately by 0, 2, 4, 6 or 8 h for reperfusion. At the end of reperfusion, the expression of PECAM-1/CD31 was detected by using flow cytometry (FCM). Simultaneously, we examined the ultrastructure changes of cells by transmission electron microscope.
  
  
  RESULTS
  The expressions of PECAM-1/CD31 in neutrophils and lymphocytes of the blood were increased after IR; the peak was at 2 h after IR, then the expressions were gradually decreased to control level at 6 and 8 h after IR. Ultrastructural morphological changes including proliferation of alveolar type II and apoptotic bodies were seen after IR, the most prominently at 2 h after IR.
  
  
  CONCLUSIONS
  PECAM-1/CD31 is involved in the pulmonary injury induced by IR.
authors.affiliation: Department of Cardiothoracic Surgery, Zhejiang Provincial People's Hospital, Hangzhou 310014, People's Republic of China. wanght1974@hotmail.com
authors.forename: H
authors.initials: H
authors.lastname: Wang
authors.forename: Z
authors.initials: Z
authors.lastname: Yan
authors.forename: L
authors.initials: L
authors.lastname: Qiu
authors.forename: Z
authors.initials: Z
authors.lastname: Hu
authors.forename: W
authors.initials: W
authors.lastname: Qian
authors.forename: L
authors.initials: L
authors.lastname: Xu
mesh_terms: D000818:Animals; D005260:Female; D008168:Lung / Q000648:ultrastructure; D055370:Lung Injury / Q000097:blood* / Q000473:pathology; D008214:Lymphocytes / Q000378:metabolism; D008297:Male; D009504:Neutrophils / Q000378:metabolism; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000097:blood*; D051381:Rats; D015427:Reperfusion Injury / Q000097:blood*
chemical_list: D019408:Platelet Endothelial Cell Adhesion Molecule-1