title: Identification of SNPs within the sheep PROP1 gene and their effects on wool traits.
abstract: Regarding mutations of PROP1 (Prophet of POU1F1) gene significantly associating with combined pituitary hormone deficiency (CPHD) in human patients and animals, PROP1 gene is a novel important candidate gene for detecting genetic variation and growth, reproduction, metabolism traits selection and breeding. The aim of this study was to detect PROP1 gene mutation of the exon 1-3 and its association with wool traits in 345 Chinese Merino sheep. In this study, on the basis of PCR-SSCP and DNA sequencing methods, ten novel SNPs within the sheep PROP1 gene, namely, AY533708: g.45A>G resulting in Glu15Glu, g.1198A>G, g.1341G>C resulting in Arg63Ser, g.1389G>A resulting in Ala79Ala, g.1402C>T resulting in Leu84Leu, g.1424A>G resulting in Asn91Ser, g.1522C>T, g.1556A>T, g.1574T>C, g.2430C>G were reported. In addition, association analysis showed that three genotypes of P4 fragment were significantly associated with fiber diameter in the analyzed population (P=0.044). These results strongly suggested that polymorphisms of the PROP1 gene could be a useful molecular marker for sheep breeding and genetics through marker-assisted selection (MAS).
authors.affiliation: College of Animal Science and Technology, Shihezi University, Shihezi, 832003, Xinjiang, People's Republic of China. Zengxiancun0993@sina.com
authors.forename: Xian-Cun
authors.initials: XC
authors.lastname: Zeng
authors.forename: Han-Ying
authors.initials: HY
authors.lastname: Chen
authors.forename: Bin
authors.initials: B
authors.lastname: Jia
authors.forename: Zong-Sheng
authors.initials: ZS
authors.lastname: Zhao
authors.forename: Wen-Qiao
authors.initials: WQ
authors.lastname: Hui
authors.forename: Zun-Bao
authors.initials: ZB
authors.lastname: Wang
authors.forename: Ying-Chun
authors.initials: YC
authors.lastname: Du
mesh_terms: D000367:Age Factors; D000818:Animals; D001483:Base Sequence; D001947:Breeding / Q000379:methods*; D002681:China; D017931:DNA Primers / Q000235:genetics; D005787:Gene Frequency; D055106:Genome-Wide Association Study; D005838:Genotype; D018398:Homeodomain Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D018807:Polymorphism, Single-Stranded Conformational; D017422:Sequence Analysis, DNA; D012756:Sheep / Q000235:genetics* / Q000502:physiology*; D014935:Wool
chemical_list: D017931:DNA Primers; D018398:Homeodomain Proteins; C102134:Prophet of Pit-1 protein