title: [Effects of endogenous carbon monoxide on gene expression profiles associated with the apoptosis of pulmonary arterial smooth muscle cells].
abstract: OBJECTIVE
  To identify the gene expression profiles associated with the apoptosis of pulmonary arterial smooth muscle cells stimulated by carbon monoxide (CO).
  
  
  METHODS
  Primary cultured Sprague-Dawley rat pulmonary arterial smooth muscle cells (PASMC) were stimulated by platelet-derived growth factor (PDGF, 20 ng/mL) and hemin (20 μmol/L). Cells were harvested after 2 hrs and Affymetrix microarrays were used to detect the gene expression profile.
  
  
  RESULTS
  Some genes associated with Map2k3 (P38) signal pathway, such as CyclinD1, CyclinH, CyclinL1, MAP2K3, Kras and Nras, were upregulated, but P27 expression was downregulated after PDGF treatment. After endogenous CO treatment, some genes associated with P53 pathway, such as Gadd45α, P21 and Trp53inp1, were upregulated.
  
  
  CONCLUSIONS
  P53 pathway probably plays an important role in apoptosis of pulmonary arterial smooth muscle cells treated with endogenous CO.
authors.affiliation: Pulmonary Vascular Remodeling Research Unit, West China Second University Hospital, Chengdu 610041, China.
authors.forename: Xiao-Jie
authors.initials: XJ
authors.lastname: Sun
authors.forename: Li
authors.initials: L
authors.lastname: Yu
authors.forename: Xiao-Qin
authors.initials: XQ
authors.lastname: Wang
authors.forename: Zhong-Qiang
authors.initials: ZQ
authors.lastname: Liu
authors.forename: Juan
authors.initials: J
authors.lastname: Wang
authors.forename: Zheng-Xiang
authors.initials: ZX
authors.lastname: Gao
authors.forename: Han-Min
authors.initials: HM
authors.lastname: Liu
mesh_terms: D000818:Animals; D017209:Apoptosis; D002248:Carbon Monoxide / Q000502:physiology*; D020869:Gene Expression Profiling; D006427:Hemin / Q000494:pharmacology; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000473:pathology*; D032389:Myocytes, Smooth Muscle / Q000473:pathology*; D011651:Pulmonary Artery / Q000473:pathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction; D016159:Tumor Suppressor Protein p53 / Q000502:physiology; D048051:p38 Mitogen-Activated Protein Kinases / Q000502:physiology
chemical_list: D016159:Tumor Suppressor Protein p53; D006427:Hemin; D002248:Carbon Monoxide; D048051:p38 Mitogen-Activated Protein Kinases