title: Nucleic acid detection using carbon nanoparticles as a fluorescent sensing platform.
abstract: In this communication, we demonstrate for the first time the proof of concept that carbon nanoparticles (CNPs) can be used as an effective fluorescent sensing platform for nucleic acid detection with selectivity down to single-base mismatch. The dye-labeled single-stranded DNA (ssDNA) probe is adsorbed onto the surface of the CNP via π-π interaction, quenching the dye. In the target assay, a double-stranded DNA (dsDNA) hybrid forms, recovering dye fluorescence.
authors.affiliation: State Key Lab of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022 Jilin, China.
authors.forename: Hailong
authors.initials: H
authors.lastname: Li
authors.forename: Yingwei
authors.initials: Y
authors.lastname: Zhang
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Jingqi
authors.initials: J
authors.lastname: Tian
authors.forename: Xuping
authors.initials: X
authors.lastname: Sun
mesh_terms: D020137:Base Pair Mismatch; D002244:Carbon / Q000737:chemistry*; D004247:DNA / Q000032:analysis* / Q000235:genetics; D015342:DNA Probes / Q000737:chemistry; D004277:DNA, Single-Stranded / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D006678:HIV / Q000235:genetics; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D015342:DNA Probes; D004277:DNA, Single-Stranded; D005456:Fluorescent Dyes; D002244:Carbon; D004247:DNA