title: Abbott ARCHITECT clinical chemistry and immunoassay systems: digoxin assays are free of interferences from spironolactone, potassium canrenoate, and their common metabolite canrenone.
abstract: Spironolactone, which is metabolized to canrenone, is often used in combination with digoxin. Potassium canrenoate is a similar drug that is also metabolized to canrenone. As a result of reported interference of spironolactone, potassium canrenoate, and their common metabolite canrenone with digoxin immunoassays, we investigated potential interference of these compounds with two relatively new digoxin assays for application on ARCHITECT clinical chemistry platforms (cDig, particle-enhanced turbidimetric inhibition immunoassay) and ARCHITECT immunoassay platforms (iDig, chemiluminescent microparticle immunoassay), both from Abbott Diagnostics. When aliquots of drug-free serum pool were supplemented with various amounts of spironolactone, potassium canrenoate, and canrenone, no apparent digoxin concentration was observed using cDig assay on ARCHITECT c4000, c8000, and c16000 or iDig assay on i1000SR and i2000SR analyzers. In addition, we observed no false increase in serum digoxin value when aliquots of a digoxin pool were further supplemented with various amounts of spironolactone, potassium canrenoate, or canrenone. We conclude that both the cDig and iDig assays on the ARCHITECT analyzers are free from interferences by spironolactone, potassium canrenoate, and canrenone.
authors.affiliation: Abbott Diagnostics, Clinical Chemistry, Irving, TX, USA.
authors.forename: Andrea
authors.initials: A
authors.lastname: DeFrance
authors.forename: David
authors.initials: D
authors.lastname: Armbruster
authors.forename: Diana
authors.initials: D
authors.lastname: Petty
authors.forename: Kelley C
authors.initials: KC
authors.lastname: Cooper
authors.forename: Amitava
authors.initials: A
authors.lastname: Dasgupta
mesh_terms: D000889:Anti-Arrhythmia Agents / Q000097:blood* / Q000378:metabolism; D002191:Canrenoic Acid / Q000097:blood* / Q000378:metabolism; D002192:Canrenone / Q000097:blood* / Q000378:metabolism; D002624:Chemistry, Clinical / Q000379:methods*; D003429:Cross Reactions; D004077:Digoxin / Q000097:blood* / Q000378:metabolism; D007118:Immunoassay / Q000379:methods*; D000451:Mineralocorticoid Receptor Antagonists / Q000097:blood; D013148:Spironolactone / Q000097:blood* / Q000378:metabolism
chemical_list: D000889:Anti-Arrhythmia Agents; D000451:Mineralocorticoid Receptor Antagonists; D013148:Spironolactone; D004077:Digoxin; D002192:Canrenone; D002191:Canrenoic Acid