title: A strategy for detecting optimal ratio of cardioprotection-dependent three compounds as quality control of guan-xin-er-hao formula.
abstract: OBJECTIVE
  We aimed to detect optimal ratio of cardioprotection-dependent absorbed bioactive compounds (ABCs) as quality control of guan-xin-er-hao (GXEH) formula extracted by various processings.
  
  
  METHODS
  Ferulic acid (F), tanshinol (T), hydroxysafflor yellow A (A), protocatechualdehyde (P) and paeoniflorin (E) in GXEH formula and FTA in blood from rat with acute myocardial infarction (AMI) were first identified by HPLC-MS/MS, and FTAPE in GXEH formulae with various herbs, extraction times and extraction water volumes were then quantitated only by HPLC.
  
  
  RESULTS
  FTAPE in various GXEH were determined. FTA were selected as GXEH's ABCs. Ratios of FTA were determined, suggesting the high (1:6.1:15.6), medium (1:1.7:15.2) and low (1:0.2:15.3) ratios. Three FTA ratios and their parent formulae ratio-dependently reduced infarct size, myocardial apoptosis and caspase-3 activity.
  
  
  CONCLUSIONS
  There is the optimal ratio of F:T:A among various formulae, contributing to the best cardioprotection. This FTA ratio was developed as quality control of GXEH formula.
authors.affiliation: Laboratory of Ethnopharmacology, Institute of Integrated Traditional Chinese and Western Medicine, Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
authors.forename: Xi
authors.initials: X
authors.lastname: Huang
authors.forename: Feng
authors.initials: F
authors.lastname: Qin
authors.forename: Ping
authors.initials: P
authors.lastname: Ren
authors.forename: Zhengyan
authors.initials: Z
authors.lastname: Zhu
authors.forename: Rong
authors.initials: R
authors.lastname: Fan
authors.forename: Chunhu
authors.initials: C
authors.lastname: Zhang
authors.forename: Hainan
authors.initials: H
authors.lastname: Zhang
authors.forename: Tao
authors.initials: T
authors.lastname: Tang
authors.forename: Juan
authors.initials: J
authors.lastname: He
authors.forename: Zhaoqian
authors.initials: Z
authors.lastname: Liu
authors.forename: Honghao
authors.initials: H
authors.lastname: Zhou
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001547:Benzaldehydes / Q000008:administration & dosage; D001565:Benzoates / Q000008:administration & dosage; D001952:Bridged-Ring Compounds / Q000008:administration & dosage; D002316:Cardiotonic Agents / Q000008:administration & dosage* / Q000737:chemistry / Q000592:standards; D053148:Caspase 3 / Q000378:metabolism; D002396:Catechols / Q000008:administration & dosage; D002599:Chalcone / Q000008:administration & dosage / Q000031:analogs & derivatives; D002626:Chemistry, Pharmaceutical; D003373:Coumaric Acids / Q000008:administration & dosage; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics / Q000592:standards; D020834:Ethnopharmacology; D005960:Glucosides / Q000008:administration & dosage; D008297:Male; D039821:Monoterpenes; D009203:Myocardial Infarction / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D008517:Phytotherapy; D011786:Quality Control; D011809:Quinones / Q000008:administration & dosage; D051381:Rats; D017207:Rats, Sprague-Dawley; D006027:Glycosides
chemical_list: D001547:Benzaldehydes; D001565:Benzoates; D001952:Bridged-Ring Compounds; D002316:Cardiotonic Agents; D053148:Caspase 3; D002396:Catechols; D002599:Chalcone; D003373:Coumaric Acids; D004365:Drugs, Chinese Herbal; D005960:Glucosides; D039821:Monoterpenes; D011809:Quinones; C534196:guan xin II; C085278:hydroxysafflor yellow A; C015423:peoniflorin; D006027:Glycosides; C005581:protocatechualdehyde; C004999:ferulic acid; C505410:Casp3 protein, rat