title: Identification of Trichinella spiralis early antigens at the pre-adult and adult stages.
abstract: Three expression cDNA libraries from Trichinella spiralis worms 14 h, 20 h and 48 h post-infection (p.i.) were screened with serum from pigs experimentally infected with 20,000 T. spiralis muscle larvae. Twenty-nine positive clones were isolated from the 14 h p.i. cDNA library, corresponding to 8 different genes. A putative excretory-secretory protein similar to that of T. pseudospiralis was identified. Three clones corresponded to a T. spiralis serine proteinase inhibitor known to be involved in diverse functions such as blood coagulation and modulation of inflammation. Screening of the 20 h p.i. cDNA library selected 167 positive clones representing 12 different sequences. The clone with the highest redundancy encoded a small polypeptide having no sequence identity with any known proteins from Trichinella or other organisms. Fourteen clones displayed sequence identity with the heat shock protein (HSP) 70. HSPs are produced as an adaptive response of the parasite to the hostile environment encountered in the host intestine but their mechanism of action is not yet well defined. From the 48 h p.i. T. spiralis cDNA library, 91 positive clones were identified representing 7 distinct sequences. Most of the positive clones showed high similarity with a member of a putative T. spiralis serine protease family. This result is consistent with a possible major role for serine proteases during invasive stages of Trichinella infection and host-parasite interactions.
authors.affiliation: AFSSA, ENVA, UPEC, LERPAZ, JRU BIPAR, 23 avenue du Général de Gaulle, 94700 Maisons-Alfort, France. a.zocevic@gmail.com
authors.forename: Aleksandar
authors.initials: A
authors.lastname: Zocevic
authors.forename: Pauline
authors.initials: P
authors.lastname: Mace
authors.forename: Isabelle
authors.initials: I
authors.lastname: Vallee
authors.forename: Radu
authors.initials: R
authors.lastname: Blaga
authors.forename: Mingyuan
authors.initials: M
authors.lastname: Liu
authors.forename: Sandrine A
authors.initials: SA
authors.lastname: Lacour
authors.forename: Pascal
authors.initials: P
authors.lastname: Boireau
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D003001:Cloning, Molecular; D005260:Female; D015723:Gene Library; D006790:Host-Parasite Interactions; D007106:Immune Sera / Q000276:immunology; D007421:Intestine, Small / Q000469:parasitology; D007814:Larva / Q000235:genetics / Q000276:immunology / Q000472:pathogenicity; D051379:Mice; D018510:RNA, Helminth / Q000235:genetics; D017422:Sequence Analysis, DNA; D057057:Serine Proteases / Q000032:analysis / Q000235:genetics / Q000276:immunology; D013047:Specific Pathogen-Free Organisms; D013552:Swine; D013553:Swine Diseases / Q000276:immunology / Q000469:parasitology*; D013997:Time Factors; D017160:Trichinella spiralis / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity; D014235:Trichinellosis / Q000276:immunology / Q000469:parasitology / Q000662:veterinary*
chemical_list: D000947:Antigens, Helminth; D007106:Immune Sera; D018510:RNA, Helminth; D057057:Serine Proteases