title: Characterization and development of a Luminex(®)-based assay for the detection of human IL-23.
abstract: BACKGROUND
  IL-23 is a cytokine produced by dendritic cells, T-cells and macrophages that plays a critical regulatory role in the inflammatory and autoimmune responses. We describe the development and preclinical validation of a highly sensitive Luminex(®) assay specific to IL-23 that is suitable for its measurement in support of early-phase clinical trials.
  
  
  RESULTS
  Intra-assay precision for the BioSource™ ELISA was under 12.3%, and under 5.2% for the eBioscience(®) ELISA. In comparison, the Luminex assays provided an intra-assay precision under 6.2%. The measured inter-assay precision was less than 15.6% for the BioSource ELISA, under 33% for the eBioscience and less than 10% for the Luminex assays.
  
  
  CONCLUSIONS
  The Luminex method described provides a way to measure IL-23 in clinical samples either as a single biomarker or as a panel of biomarkers. The assay should prove useful to scientists and clinicians investigating the biology of IL-23 and to those needing to monitor changes in IL-23 as part of a clinical study.
authors.affiliation: Bristol-Myers Squibb Co., Princeton, NJ, USA.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Rizzi
authors.forename: Yan J
authors.initials: YJ
authors.lastname: Zhang
authors.forename: Robert
authors.initials: R
authors.lastname: Latek
authors.forename: Russell
authors.initials: R
authors.lastname: Weiner
authors.forename: Paul W
authors.initials: PW
authors.lastname: Rhyne
mesh_terms: D000818:Animals; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D053759:Interleukin-23 / Q000097:blood* / Q000378:metabolism; D057230:Limit of Detection; D008863:Microspheres; D051381:Rats; D015203:Reproducibility of Results; D015398:Signal Transduction
chemical_list: D053759:Interleukin-23