title: Rapid chemical ligation of oligonucleotides by the Diels-Alder reaction.
abstract: Extremely fast and efficient Diels-Alder chemical ligation of furan and maleimide oligonucleotides has been carried out in aqueous buffer. It was possible to ligate three oligonucleotides simultaneously in a controlled manner with the aid of a complementary splint. The templated reactions proceeded within 1 min at room temperature whereas non-templated reactions were slow and incomplete. Rapid and clean methods of DNA ligation such as the one demonstrated here have potential uses in biology and nanotechnology.
authors.affiliation: School of Chemistry, University of Southampton, Highfield, Southampton, UK SO17 1BJ. ahes@soton.ac.uk
authors.forename: Afaf H
authors.initials: AH
authors.lastname: El-Sagheer
authors.forename: Vee Vee
authors.initials: VV
authors.lastname: Cheong
authors.forename: Tom
authors.initials: T
authors.lastname: Brown
mesh_terms: D015394:Molecular Structure; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D013997:Time Factors
chemical_list: D009838:Oligodeoxyribonucleotides