title: An improved nitrilase-mediated bioprocess for synthesis of nicotinic acid from 3-cyanopyridine with hyperinduced Nocardia globerula NHB-2.
abstract: Nitrilase of Nocardia globerula NHB-2 was induced by short-chain aliphatic nitriles (valeronitrile > isobutyronitrile > butyronitrile > propionitrile) and exhibited activity towards aromatic nitriles (benzonitrile > 3-cyanopyridine > 4-cyanopyridine > m-tolunitrile > p-tolunitrile). Hyperinduction of nitrilase (6.67 U mg (DCW) (-1), 18.7 U mL(-1)) was achieved in short incubation time (30 h, 30°C) through multiple feeding of isobutyronitrile in the growth medium. The nitrilase of this organism exhibits both substrate and product inhibition effects. In a fed batch reaction at 1 L scale using hyperinduced resting cells corresponding to 10 U mL(-1) nitrilase activity (1.5 mg(DCW) mL(-1)), a total of 123.11 g nicotinic acid was produced at a rate of 24 g h(-1) g (DCW) (-1).
authors.affiliation: Department of Biotechnology, Himachal Pradesh University, Summer Hill, Shimla, 171005, India.
authors.forename: Nitya Nand
authors.initials: NN
authors.lastname: Sharma
authors.forename: Monica
authors.initials: M
authors.lastname: Sharma
authors.forename: Tek Chand
authors.initials: TC
authors.lastname: Bhalla
mesh_terms: D000619:Aminohydrolases / Q000378:metabolism*; D003470:Culture Media; D009525:Niacin / Q000096:biosynthesis*; D009570:Nitriles / Q000378:metabolism; D009615:Nocardia / Q000201:enzymology*; D011725:Pyridines / Q000378:metabolism*
chemical_list: D003470:Culture Media; D009570:Nitriles; D011725:Pyridines; D009525:Niacin; C022246:isobutyronitrile; D000619:Aminohydrolases; C021867:nitrilase; C020729:3-cyanopyridine