title: [The expression variations of connective tissue growth factor and cyclinD1 in pulmonary vascular remodeling induced by cigarette smoke in rats].
abstract: OBJECTIVE
  To investigate the expression variations of connective tissue growth factor (CTGF) and cyclin D1 in pulmonary vasculature in rats exposed to cigarette smoke and their roles in pulmonary vascular remodeling.
  
  
  METHODS
  Twenty-four male Wistar rats were randomly divided into 4 groups: 1 control group (C group) and 3 smoke exposure groups (S2w, S4w, S8w group). Arterial partial pressure of oxygen was measured. Pulmonary artery remodeling was observed by Hematoxylin-Eosin staining and the percentage of muscularised small pulmonary arteries. Immunohistochemistry methods were performed to observe CTGF and cyclin D1 expressions in pulmonary artery smooth muscle. Real time quantitative RT-PCR and Western blot analysis were used for detection of mRNA and protein expressions in pulmonary artery smooth muscle.
  
  
  RESULTS
  There was no significant difference in arterial partial pressure of oxygen among all groups. The percentage of muscularised small vessels and W/T were significantly increased in S2w, S4w and S8w group compared to control group (P < 0.05). Compared to control group, significant increases of CTGF and cyclinD1 expressions in smoke exposure groups were observed (P < 0.05). The expressions of CTGF and cyclinD1 were significantly positively correlated with the severity of pulmonary vascular muscularization, and there was statistically positive correlation between the expression of CTGF and cyclinD1.
  
  
  CONCLUSIONS
  CTGF and cyclinD1 expressions significantly were upregulated in pulmonary arteries from rats exposed to cigarette smoke (2-8w) and there was a significant positive correlation between their expressions. Their expression variations may be associated with abnormal proliferation of pulmonary artery smooth muscle cells induced by cigarette smoke.
authors.affiliation: Department of Respiratory Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Ran
authors.initials: R
authors.lastname: Wang
authors.forename: Yong-jian
authors.initials: YJ
authors.lastname: Xu
authors.forename: Xian-sheng
authors.initials: XS
authors.lastname: Liu
authors.forename: Da-xiong
authors.initials: DX
authors.lastname: Zeng
authors.forename: Min
authors.initials: M
authors.lastname: Xiang
mesh_terms: D056151:Airway Remodeling; D000818:Animals; D055513:Connective Tissue Growth Factor / Q000378:metabolism*; D019938:Cyclin D1 / Q000378:metabolism*; D019570:Inhalation Exposure; D008168:Lung / Q000098:blood supply; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015208:Smoke Inhalation Injury / Q000378:metabolism*; D012907:Smoking / Q000009:adverse effects*
chemical_list: C526316:CCN2 protein, rat; C530106:Ccnd1 protein, rat; D019938:Cyclin D1; D055513:Connective Tissue Growth Factor