title: A novel zinc-carboxypeptidase SURO-1 regulates cuticle formation and body morphogenesis in Caenorhabditis elegans.
abstract: Cuticle formation and molting are critical for the development of Caenorhabditis elegans. To understand cuticle formation more clearly, we screened for suppressors in transgenic worms that expressed dominant ROL-6 collagen proteins. The suro-1 mutant, which is mild dumpy, exhibited a different ROL-6::GFP localization pattern compared to other Dpy mutants. We identified mutations in three suro-1 mutants, and found that suro-1 (ORF R11A5.7) encodes a putative zinc-carboxypeptidase homologue. The expression of this enzyme in the hypodermis and the genetic interactions between this enzyme and other collagen-modifying enzyme mutants suggest a regulatory role in collagen processing and cuticle organization for this novel carboxypeptidase. These findings aid our understanding of cuticle formation during worm development.
authors.affiliation: Cancer Experimental Resources Branch, National Cancer Center, Ilsandong-gu, Goyang-si, Gyeonggi-do, Republic of Korea.
authors.forename: Tai Hoon
authors.initials: TH
authors.lastname: Kim
authors.forename: Yeon Jung
authors.initials: YJ
authors.lastname: Kim
authors.forename: Jin Won
authors.initials: JW
authors.lastname: Cho
authors.forename: Jaegal
authors.initials: J
authors.lastname: Shim
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism*; D002268:Carboxypeptidases / Q000235:genetics / Q000378:metabolism*; D003094:Collagen / Q000235:genetics / Q000378:metabolism; D004817:Epidermis / Q000254:growth & development / Q000378:metabolism*; D005260:Female; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D008856:Microscopy, Fluorescence; D009024:Morphogenesis; D009154:Mutation; D034622:RNA Interference; D015032:Zinc / Q000378:metabolism
chemical_list: D029742:Caenorhabditis elegans Proteins; C450827:DPY-11 protein, C elegans; D008565:Membrane Proteins; C098282:rol-6 protein, C elegans; D049452:Green Fluorescent Proteins; D003094:Collagen; D002268:Carboxypeptidases; C556233:SURO-1 protein, C elegans; D015032:Zinc