title: Molecular cloning, expression and antiviral activity of porcine interleukin-29 (poIL-29).
abstract: Human interleukin-29 (IL-29) is a recently discovered cytokine displaying antiviral activity against a broad spectrum of viruses, designated as interferon (IFN)-λ1. We here report the molecular cloning, expression and antiviral activity of porcine IL-29 (poIL-29). The full-length poIL-29 cDNA sequence encoded 191 amino acids with a 19 amino acid signal peptide. Sequence alignments showed that poIL-29 had amino acid sequence similarity to wolf (76%), bear (75%), horse (75%), orangutan (73%), human (72%), cat (72%) and dog (70%) IL-29. The poIL-29 without signal anchor sequence was efficiently expressed as a 6 × HIS fusion protein in Escherichia coli and the antiviral activity of the purified recombinant protein was 1.8 × 10(3)U/mg protein. The purified recombinant poIL-29 also exhibited significant antiviral effects against porcine reproductive and respiratory syndrome virus and pseudorabies virus in a dose-dependent manner, suggesting that poIL-29 is a potential antiviral agent against swine infectious diseases.
authors.affiliation: Division of Animal Infectious Diseases, State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Dang
authors.initials: D
authors.lastname: Wang
authors.forename: Liurong
authors.initials: L
authors.lastname: Fang
authors.forename: Fuwei
authors.initials: F
authors.lastname: Zhao
authors.forename: Rui
authors.initials: R
authors.lastname: Luo
authors.forename: Huanchun
authors.initials: H
authors.lastname: Chen
authors.forename: Shaobo
authors.initials: S
authors.lastname: Xiao
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D006801:Humans; D007378:Interleukins / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D013552:Swine / Q000235:genetics* / Q000276:immunology*; D013553:Swine Diseases / Q000235:genetics / Q000276:immunology; D014777:Virus Diseases / Q000276:immunology
chemical_list: D007378:Interleukins