title: Few constraints limit the design of quinone methide-oligonucleotide self-adducts for directing DNA alkylation.
abstract: Nucleotide sequences minimally containing adenosine, cytosine or guanosine are sufficient to form intrastrand oligonucleotide quinone methide self-adducts reversibly for subsequent alkylation of complementary DNA. The general lack of sequence restrictions should now allow for alkylation of most any target of interest although reaction is most efficient when the self-adducts contain guanine residues and do not form hairpin structures.
authors.affiliation: Department of Chemistry and Biochemistry, University of Maryland, College Park, MD 20742, USA.
authors.forename: Clifford S
authors.initials: CS
authors.lastname: Rossiter
authors.forename: Emilia
authors.initials: E
authors.lastname: Modica
authors.forename: Dalip
authors.initials: D
authors.lastname: Kumar
authors.forename: Steven E
authors.initials: SE
authors.lastname: Rokita
mesh_terms: D000478:Alkylation; D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D018736:DNA Adducts / Q000737:chemistry*; D045563:Indolequinones / Q000737:chemistry*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*
chemical_list: D018736:DNA Adducts; D045563:Indolequinones; D009838:Oligodeoxyribonucleotides; C068040:quinone methide; D004247:DNA