title: Disturbed apolipoprotein A-I-containing lipoproteins in fish-eye disease are improved by the lecithin:cholesterol acyltransferase produced by gene-transduced adipocytes in vitro.
abstract: We report the in vitro efficacy of recombinant LCAT produced by lcat gene-transduced proliferative adipocytes (ccdPA/lcat), which has been developed for enzyme replacement therapy. ApoA-I-specific immunodetection in combination with 1D and 2D gel electrophoreses showed that the disturbed high-density lipoprotein subpopulation profile was clearly ameliorated by the in vitro incubation with ccdPA/lcat-derived recombinant LCAT. Thus, these results using ccdPA/lcat strongly suggest the cell implantation could contribute the enzyme replacement for the patients with LCAT deficiency.
authors.affiliation: Department of Genome Research and Clinical Application, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chiba, Japan.
authors.forename: Sakiyo
authors.initials: S
authors.lastname: Asada
authors.forename: Masayuki
authors.initials: M
authors.lastname: Kuroda
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Aoyagi
authors.forename: Hideaki
authors.initials: H
authors.lastname: Bujo
authors.forename: Shigeaki
authors.initials: S
authors.lastname: Tanaka
authors.forename: Shunichi
authors.initials: S
authors.lastname: Konno
authors.forename: Masami
authors.initials: M
authors.lastname: Tanio
authors.forename: Itsuko
authors.initials: I
authors.lastname: Ishii
authors.forename: Masayuki
authors.initials: M
authors.lastname: Aso
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Saito
mesh_terms: D017667:Adipocytes / Q000201:enzymology*; D016632:Apolipoprotein A-I / Q000235:genetics* / Q000378:metabolism*; D002478:Cells, Cultured; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D066298:In Vitro Techniques; D007863:Lecithin Cholesterol Acyltransferase Deficiency / Q000201:enzymology* / Q000235:genetics*; D008297:Male; D007862:Phosphatidylcholine-Sterol O-Acyltransferase / Q000097:blood / Q000235:genetics / Q000378:metabolism*
chemical_list: D016632:Apolipoprotein A-I; D007862:Phosphatidylcholine-Sterol O-Acyltransferase