title: Carboxypeptidase from Streptomyces bikiniensis: primary structure, isolation, and properties.
abstract: A metallocarboxypeptidase produced by Streptomyces bikiniensis 27 strain (VKPM Ac-1783) (CPSb) was purified and characterized. The enzyme cleaves both basic and hydrophobic C-terminal amino acid residues from synthetic peptides, that is, it possesses specificity of mammalian carboxypeptidases A and B. The enzyme also hydrolyzes peptides bearing glutamic acid at the C-end. CPSb exhibits its maximal activity at pH 7.0-7.6 and 55°C. The nucleotide sequence encoding the mature CPSb in S. bikiniensis 27 (VKPM Ac-1783) genome (Accession No. GU362077) was determined. It is shown that the primary structure of the mature enzyme has a moderate degree of identity with orthologs from Streptomyces griseus (79% identity) and Streptomyces avermitilis (85% identity).
authors.affiliation: Institute for Genetics and Selection of Industrial Microorganisms, Moscow, 117545, Russia.
authors.forename: A V
authors.initials: AV
authors.lastname: Serkina
authors.forename: I A
authors.initials: IA
authors.lastname: Zalunin
authors.forename: E I
authors.initials: EI
authors.lastname: Levitin
authors.forename: T A
authors.initials: TA
authors.lastname: Voejkova
authors.forename: B V
authors.initials: BV
authors.lastname: Tyaglov
authors.forename: L M
authors.initials: LM
authors.lastname: Novikova
authors.forename: L K
authors.initials: LK
authors.lastname: Emeljanova
authors.forename: G E
authors.initials: GE
authors.lastname: Konstantinova
authors.forename: G G
authors.initials: GG
authors.lastname: Chestukhina
mesh_terms: D000595:Amino Acid Sequence; D002268:Carboxypeptidases / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D013302:Streptomyces / Q000201:enzymology* / Q000378:metabolism; D013305:Streptomyces griseus / Q000201:enzymology / Q000378:metabolism; D013379:Substrate Specificity; D013696:Temperature
chemical_list: D010455:Peptides; D002268:Carboxypeptidases