title: Stress effects on AVT and CRF systems in two strains of rainbow trout (Oncorhynchus mykiss) divergent in stress responsiveness.
abstract: The aim for this study was to examine whether the F4 generation of two strains of rainbow trout divergent in their plasma cortisol response to confinement stress (HR: high responder or LR: low responder) would also differ in stress-induced effects on forebrain concentrations of mRNA for corticotropin-releasing factor (CRF), arginine vasotocin (AVT), CRF receptor type 1 (CRF-R1), CRF receptor type 2 (CRF-R2) and AVT receptor (AVT-R). In addition, plasma cortisol concentrations, brainstem levels of monoamines and monoamine metabolites, and behaviour during confinement were monitored. The results confirm that HR and LR trout differ in their cortisol response to confinement and show that fish of these strains also differ in their behavioural response to confinement. The HR trout displayed significantly higher locomotor activity while in confinement than LR trout. Moreover, following 180 min of confinement HR fish showed significantly higher forebrain concentrations of CRF mRNA than LR fish. Also, when subjected to 30 min of confinement HR fish showed significantly lower CRF-R2 mRNA concentrations than LR fish, whereas there were no differences in CRF-R1, AVT or AVT-R mRNA expression between LR and HR fish either at 30 or 180 min of confinement. Differences in the expression of CRF and CRF-R2 mRNA may be related to the divergence in stress coping displayed by these rainbow trout strains.
authors.affiliation: Evolutionary Biology Centre, Department of Comparative Physiology, Uppsala University, Norbyvägen 18A, SE-752 36 Uppsala, Sweden.
authors.forename: Tobias
authors.initials: T
authors.lastname: Backström
authors.forename: Joachim
authors.initials: J
authors.lastname: Schjolden
authors.forename: Øyvind
authors.initials: Ø
authors.lastname: Øverli
authors.forename: Per-Ove
authors.initials: PO
authors.lastname: Thörnqvist
authors.forename: Svante
authors.initials: S
authors.lastname: Winberg
mesh_terms: D000223:Adaptation, Psychological / Q000502:physiology*; D000704:Analysis of Variance; D000818:Animals; D001933:Brain Stem / Q000378:metabolism*; D003346:Corticotropin-Releasing Hormone / Q000235:genetics / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D006854:Hydrocortisone / Q000097:blood; D006897:Hydroxyindoleacetic Acid / Q000378:metabolism; D009043:Motor Activity / Q000502:physiology; D017686:Oncorhynchus mykiss / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018019:Receptors, Corticotropin-Releasing Hormone / Q000235:genetics / Q000378:metabolism; D012701:Serotonin / Q000378:metabolism; D013045:Species Specificity; D018709:Statistics, Nonparametric; D013312:Stress, Physiological / Q000502:physiology*; D014668:Vasotocin / Q000235:genetics / Q000378:metabolism*; D000099052:CRF Receptor, Type 1
chemical_list: C101531:CRF receptor type 2; D012333:RNA, Messenger; D018019:Receptors, Corticotropin-Releasing Hormone; D012701:Serotonin; D006897:Hydroxyindoleacetic Acid; D000099052:CRF Receptor, Type 1; D003346:Corticotropin-Releasing Hormone; D014668:Vasotocin; D006854:Hydrocortisone