title: Integron prevalence and diversity in manured soil.
abstract: The levels of integron abundance and diversity in soil amended with pig slurry were studied. Real-time PCR illustrated a significant increase in class 1 integron prevalence after slurry application, with increased prevalence still evident at 10 months after application. Culture-dependent data revealed 10 genera, including putative human pathogens, carrying class 1 and 2 integrons.
authors.affiliation: Department of Biological Sciences, University of Warwick, Gibbet Hill, Coventry, West Midlands CV4 7AL, United Kingdom.
authors.forename: K G
authors.initials: KG
authors.lastname: Byrne-Bailey
authors.forename: W H
authors.initials: WH
authors.lastname: Gaze
authors.forename: L
authors.initials: L
authors.lastname: Zhang
authors.forename: P
authors.initials: P
authors.lastname: Kay
authors.forename: A
authors.initials: A
authors.lastname: Boxall
authors.forename: P M
authors.initials: PM
authors.lastname: Hawkey
authors.forename: E M H
authors.initials: EM
authors.lastname: Wellington
mesh_terms: D000383:Agriculture / Q000379:methods; D000818:Animals; D001419:Bacteria / Q000145:classification / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D014644:Genetic Variation; D032023:Integrons; D008372:Manure / Q000382:microbiology*; D054892:Metagenome; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D015995:Prevalence; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013552:Swine
chemical_list: D004269:DNA, Bacterial; D008372:Manure