title: Bone formation on carbon nanotube composite.
abstract: The effects of a layer-by-layer assembled carbon nanotube composite (CNT-comp) on osteoblasts in vitro and bone tissue in vivo in rats were studied. The effects of CNT-comp on osteoblasts were compared against the effects by commercially pure titanium (cpTi) and tissue culture dishes. Cell proliferation on the CNT-comp and cpTi were similar. However, cell differentiation, measured by alkaline phosphatase activity and matrix mineralization, was better on the CNT-comp. When implanted in critical-sized rat calvarial defect, the CNT-comp permitted bone formation and bone repair without signs of rejection or inflammation. These data indicate that CNT-comp may be a promising substrate for use as a bone implant or as a scaffold for tissue engineering.
authors.affiliation: Department Bioproducts and Biosystems Engineering, University of Minnesota, Saint Paul, Minnesota, USA.
authors.forename: Mrinal
authors.initials: M
authors.lastname: Bhattacharya
authors.forename: Patcharaporn
authors.initials: P
authors.lastname: Wutticharoenmongkol-Thitiwongsawet
authors.forename: Darryl T
authors.initials: DT
authors.lastname: Hamamoto
authors.forename: Dongjin
authors.initials: D
authors.lastname: Lee
authors.forename: Tianhong
authors.initials: T
authors.lastname: Cui
authors.forename: Hari S
authors.initials: HS
authors.lastname: Prasad
authors.forename: Mansur
authors.initials: M
authors.lastname: Ahmad
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D001842:Bone and Bones / Q000166:cytology / Q000378:metabolism; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D006801:Humans; D008297:Male; D008422:Materials Testing; D037742:Nanotubes, Carbon / Q000737:chemistry*; D010006:Osteoblasts / Q000166:cytology / Q000502:physiology*; D010012:Osteogenesis / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry; D014025:Titanium / Q000494:pharmacology
chemical_list: D001672:Biocompatible Materials; D037742:Nanotubes, Carbon; D014025:Titanium; D000469:Alkaline Phosphatase