title: Increased advanced oxidation of protein products and enhanced total antioxidant capacity in plasma by action of toxins of Escherichia coli STEC.
abstract: Shiga toxin (Stx) and hemolysin (Hly) of Escherichia coli O157:H7 produced an increase of reactive oxygen species (ROS) in normal human blood. In vitro assays showed that stimuli of ROS with these toxins oxidized proteins to carbonyls in plasma and raised the degradation of oxidized macromolecules, with the AOPP/carbonyl relationship also increasing. The oxidative stress generated by toxins during the Hemolytic Uremic Syndrome (HUS) produced oxidation of blood proteins with a rise in advanced oxidation protein products (AOPP) in children with HUS. There was a response from the antioxidant system in these patients, evaluated through the determination of the total antioxidant capacity of plasma by the Ferric Reducing Antioxidant Power (FRAP), which reduced the stimuli of ROS during in vitro incubation with Stx or Hly. The application of natural antioxidants was sufficient to reduce in vitro the oxidative stress provoked by both toxins in blood.
authors.affiliation: Departamento de Farmacia, Facultad Ciencias Químicas, Universidad Nacional Córdoba, Córdoba, Argentina.
authors.forename: V
authors.initials: V
authors.lastname: Aiassa
authors.forename: J L
authors.initials: JL
authors.lastname: Baronetti
authors.forename: P L
authors.initials: PL
authors.lastname: Paez
authors.forename: A I
authors.initials: AI
authors.lastname: Barnes
authors.forename: C
authors.initials: C
authors.lastname: Albrecht
authors.forename: G
authors.initials: G
authors.lastname: Pellarin
authors.forename: A J
authors.initials: AJ
authors.lastname: Eraso
authors.forename: I
authors.initials: I
authors.lastname: Albesa
mesh_terms: D000975:Antioxidants / Q000378:metabolism* / Q000494:pharmacology; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D001798:Blood Proteins / Q000378:metabolism*; D002648:Child; D019453:Escherichia coli O157 / Q000378:metabolism / Q000472:pathogenicity*; D029968:Escherichia coli Proteins / Q000302:isolation & purification / Q000633:toxicity*; D005638:Fruit / Q000737:chemistry; D006460:Hemolysin Proteins / Q000302:isolation & purification / Q000633:toxicity*; D006463:Hemolytic-Uremic Syndrome / Q000097:blood / Q000378:metabolism; D006801:Humans; D007962:Leukocytes / Q000187:drug effects / Q000378:metabolism; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology; D029862:Prosopis / Q000737:chemistry; D050050:Protein Carbonylation / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000097:blood; D022621:Shiga Toxin / Q000302:isolation & purification / Q000633:toxicity*; D031957:Ziziphus / Q000737:chemistry
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D001798:Blood Proteins; D029968:Escherichia coli Proteins; D006460:Hemolysin Proteins; C036507:Hlya protein, E coli; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D022621:Shiga Toxin