title: High-cell density-induced VCAM1 expression inhibits the migratory ability of mesenchymal stem cells.
abstract: MSCs (mesenchymal stem cells) migrate into damaged tissue and then proliferate and differentiate into various cell lineages to regenerate bone, cartilage, fat and muscle. Cell-cell adhesion of MSCs is essential for the MSC-dependent tissue regeneration after their homing into a damaged tissue. However, it remains to be elucidated what kinds of adhesion molecules play important roles in the cell-cell communication between MSCs. In order to identify adhesion molecules that facilitate mutual contact between MSCs, a comprehensive analysis of mRNA expression in adhesion molecules was performed by comparing profiles of expression status of adhesion molecules in MSCs at low- and high-cell density. We found that the expression level of VCAM1 (vascular cell adhesion molecule-1)/CD106 was clearly up-regulated in the human bone marrow-derived MSCs-UE7T-13 cells - under a condition of high cell density. Intriguingly, the migratory ability of the cells was clearly accelerated by a knockdown of VCAM1. Furthermore, the migratory ability of UE7T-13 cells was decreased by the over expression of exogenous VCAM1. In addition, the high cell density-induced expression of VCAM1 was clearly suppressed by NF-κB (nuclear factor-κB) signalling-related protein kinase inhibitors such as an IKK-2 (IκB kinase-2) inhibitor VI. In conclusion, the high cell density-induced VCAM1 expression through the NF-κB pathway inhibits the migratory ability of human bone marrow-derived MSCs.
authors.affiliation: Division of Biochemistry and Molecular Genetics, Department of Oral Biology, Iwate Medical University School of Dentistry, Morioka, Iwate, Japan.
authors.forename: Soko
authors.initials: S
authors.lastname: Nishihira
authors.forename: Naoto
authors.initials: N
authors.lastname: Okubo
authors.forename: Noriko
authors.initials: N
authors.lastname: Takahashi
authors.forename: Akira
authors.initials: A
authors.lastname: Ishisaki
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Sugiyama
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Chosa
mesh_terms: D002448:Cell Adhesion; D002452:Cell Count; D002460:Cell Line; D002465:Cell Movement; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics; D019010:Vascular Cell Adhesion Molecule-1 / Q000235:genetics* / Q000378:metabolism
chemical_list: D016328:NF-kappa B; D047428:Protein Kinase Inhibitors; D012333:RNA, Messenger; D019010:Vascular Cell Adhesion Molecule-1