title: Immobilization of acetylcholinesterase in lipid membranes deposited on self-assembled monolayers.
abstract: Human red blood cell acetylcholinesterase was incorporated into planar lipid membranes deposited on alkanethiol self-assembled monolayers (SAMs) on gold substrates. Activity of the protein in the membrane was detected with a standard photometric assay and was determined to be similar to the protein in detergent solution or incorporated in lipid vesicles. Monolayer and bilayer lipid membranes were generated by fusing liposomes to hydrophobic and hydrophilic SAMs, respectively. Liposomes were formed by the injection method using the lipid dimyristoylphosphatidylcholine (DMPC). The formation of alkanethiol SAMs and lipid monolayers on SAMs was confirmed by sessile drop goniometry, ellipsometry, and electrochemical impedance spectroscopy. In this work, we report acetylcholinesterase immobilization in lipid membranes deposited on SAMs formed on the gold surface and compare its activity to enzyme in solution.
authors.affiliation: Worcester Polytechnic Institute, 100 Institute Road, Worcester, Massachusetts 01609, United States.
authors.forename: Eftim
authors.initials: E
authors.lastname: Milkani
authors.forename: Aung M
authors.initials: AM
authors.lastname: Khaing
authors.forename: Fei
authors.initials: F
authors.lastname: Huang
authors.forename: Daniel G
authors.initials: DG
authors.lastname: Gibson
authors.forename: Scott
authors.initials: S
authors.lastname: Gridley
authors.forename: Norman
authors.initials: N
authors.lastname: Garceau
authors.forename: Christopher R
authors.initials: CR
authors.lastname: Lambert
authors.forename: W Grant
authors.initials: WG
authors.lastname: McGimpsey
mesh_terms: D000110:Acetylcholinesterase / Q000737:chemistry* / Q000378:metabolism; D002462:Cell Membrane / Q000737:chemistry* / Q000378:metabolism; D058266:Dielectric Spectroscopy; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008051:Lipid Bilayers / Q000737:chemistry* / Q000378:metabolism; D013438:Sulfhydryl Compounds / Q000737:chemistry; D053835:Unilamellar Liposomes / Q000737:chemistry / Q000378:metabolism
chemical_list: D004800:Enzymes, Immobilized; D008051:Lipid Bilayers; D013438:Sulfhydryl Compounds; D053835:Unilamellar Liposomes; D000110:Acetylcholinesterase; D004134:Dimyristoylphosphatidylcholine