title: Functional and histological comparison of rat liver preserved in University of Wisconsin solution compared with tissue preserved in a novel solution.
abstract: An isolated perfused rat liver model was used to investigate biochemical and histologic changes during 2 hours of reperfusion after 24 hours of cold storage to compare Leeds solution (LS) with University of Wisconsin solution (UW). Compared with livers stored in UW, those perfused with LS showed significantly higher bile flow and lower enzyme production (P < .05 by 1-way analysis of variance). For example, after 120 minutes, alanine aminotransferase results were: LS 38.9 U/L vs UW 66.8 U/L and bile flows were LS 10.3 μg/15 min/g liver vs UW 9.2 μg/15 min/g liver. Histologically the reticulin breakdown was greater and its reformation slower in UW-preserved livers. Liver tissue was viable in both groups, as shown by the increased glycogen content after reperfusion in both groups, but seen at a higher rate among LS, perfused livers. In conclusion, LS compared favorably with UW to prevent ischemic damage and so could offer an alternative perfusion medium to UW.
authors.affiliation: Transplant Science Group, Department of Hepatology and Transplantation, St. James’s University Hospital, Leeds, West Yorkshire, LS9 7TF, UK. clairecorps@hotmail.com
authors.forename: C L
authors.initials: CL
authors.lastname: Corps
authors.forename: I
authors.initials: I
authors.lastname: Ahmed
authors.forename: S
authors.initials: S
authors.lastname: McKenzie
authors.forename: M
authors.initials: M
authors.lastname: Shires
authors.forename: D J
authors.initials: DJ
authors.lastname: Potts
authors.forename: J P A
authors.initials: JP
authors.lastname: Lodge
mesh_terms: D000241:Adenosine / Q000494:pharmacology; D000410:Alanine Transaminase / Q000378:metabolism; D000493:Allopurinol / Q000494:pharmacology; D000704:Analysis of Variance; D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism; D001646:Bile / Q000378:metabolism; D050377:Cold Ischemia / Q000009:adverse effects; D005978:Glutathione / Q000494:pharmacology; D006003:Glycogen / Q000378:metabolism; D066298:In Vitro Techniques; D007328:Insulin / Q000494:pharmacology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008099:Liver / Q000098:blood supply* / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D009926:Organ Preservation / Q000379:methods*; D019188:Organ Preservation Solutions / Q000494:pharmacology*; D011887:Raffinose / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000209:etiology / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D012155:Reticulin / Q000378:metabolism; D013997:Time Factors; D014022:Tissue Survival
chemical_list: D007328:Insulin; D019188:Organ Preservation Solutions; D012155:Reticulin; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; D006003:Glycogen; D007770:L-Lactate Dehydrogenase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose