title: Coating of titanium implant materials with thin polymeric films for binding the signaling protein BMP2.
abstract: A fast and simple approach for immobilization using copolymers as interlayers is reported. The synthesized copolymers form stable self-assembled layers on implant materials like, e.g., titanium in a simple coating/drying/washing sequence and have functional groups which can bind proteins from an aqueous solution. The copolymer films have been characterized via ellipsometry and contact angle measurements and were tested for biocompatibility. An immunoassay was used to determine the amount of BMP2 and demonstrated an approximately 10-fold increase as compared to previously used self-assembled monolayers. A BMP2-responsive cell line with luciferase detection was used to determine the biological activity of the bound signaling protein.
authors.affiliation: Institute for Technical Chemistry, Braunschweig University of Technology, Hans-Sommer-Straße 10, 38106 Braunschweig, Germany.
authors.forename: Corinna
authors.initials: C
authors.lastname: Lorenz
authors.forename: Andrea
authors.initials: A
authors.lastname: Hoffmann
authors.forename: Gerhard
authors.initials: G
authors.lastname: Gross
authors.forename: Henning
authors.initials: H
authors.lastname: Windhagen
authors.forename: Philip
authors.initials: P
authors.lastname: Dellinger
authors.forename: Kai
authors.initials: K
authors.lastname: Möhwald
authors.forename: Wibke
authors.initials: W
authors.lastname: Dempwolf
authors.forename: Henning
authors.initials: H
authors.lastname: Menzel
mesh_terms: D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000378:metabolism*; D049109:Cell Proliferation / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000494:pharmacology*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D055767:Immobilized Proteins / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D058105:Polymerization / Q000187:drug effects; D011108:Polymers / Q000494:pharmacology*; D019736:Prostheses and Implants; D011485:Protein Binding / Q000187:drug effects; D015398:Signal Transduction / Q000187:drug effects*; D014025:Titanium / Q000494:pharmacology*
chemical_list: D055396:Bone Morphogenetic Protein 2; D020099:Coated Materials, Biocompatible; D055767:Immobilized Proteins; D011108:Polymers; C009495:titanium dioxide; D014025:Titanium