title: Non-label homogeneous protein detection based on laser interferometric photo-thermal displacement measurement using aptamers.
abstract: Photo-thermal displacement measurement by laser interferometry involves the measurement of temperature change caused by illumination of the sample. To develop a system of detecting unlabeled homogeneous proteins based on laser interferometric measurement of photo-thermal displacement, we studied the interaction between aptamers and their target molecules by using thrombin and the thrombin aptamer as a model target and ligand, respectively. Because of the energy consumed by aptamer-thrombin interactions, the signals obtained from solutions containing aptamer-thrombin mixtures varied depending on the thrombin concentration. We propose that this method involving the use of aptamers and photo-thermal displacement measurement will provide a biomolecular detection system for rapid diagnosis.
authors.affiliation: Department of Biotechnology, Tokyo University of Agriculture and Technology, Koganei, Tokyo, Japan.
authors.forename: Kaori
authors.initials: K
authors.lastname: Tsukakoshi
authors.forename: Daisuke
authors.initials: D
authors.lastname: Ogasawara
authors.forename: Eiji
authors.initials: E
authors.lastname: Takahashi
authors.forename: Ryo
authors.initials: R
authors.lastname: Katayama
authors.forename: Kazunori
authors.initials: K
authors.lastname: Ikebukuro
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods; D007368:Interferometry; D011506:Proteins / Q000737:chemistry*
chemical_list: D052157:Aptamers, Nucleotide; D011506:Proteins; C081676:thrombin aptamer