title: Comprehensive analysis of low-abundance proteins in human urinary exosomes using peptide ligand library technology, peptide OFFGEL fractionation and nanoHPLC-chip-MS/MS.
abstract: Human urinary exosomes are 30-100 nm vesicles that originate as the internal vesicles in multivesicular bodies from every renal epithelial cell type facing the urinary track and may serve as a suitable noninvasive starting material for biomarker discovery relevant to a variety of renal disease. To comprehensively explore the low-abundance proteome, combinatorial peptide ligand libraries, combined with peptide OFFGEL electrophoresis were employed for the enrichment and separation of relatively low-abundant proteins in urinary exosomes. After analysis by nanoHPLC-chip-MS/MS, 512 proteins were identified, including a large number of proteins with extreme molecular weight or extreme pI value, which could not be well mapped by using traditional 2-D-gel-based separation methods. This in-depth analysis of low-abundant proteins in urinary exosomes led to an increased understanding of molecular composition of these little vesicles and may be helpful for the discovery of novel biomarker. Our work also provides an effective strategy of concentration and identification of low-abundance proteome from complex bio-samples.
authors.affiliation: College of Pharmacy, Chongqing Medical University, Chongqing, PR China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Li
authors.forename: Feng
authors.initials: F
authors.lastname: Qiu
authors.forename: Zongyin
authors.initials: Z
authors.lastname: Qiu
mesh_terms: D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D020650:Combinatorial Chemistry Techniques / Q000379:methods*; D004586:Electrophoresis / Q000379:methods*; D055354:Exosomes / Q000737:chemistry*; D006801:Humans; D016253:Microscopy, Immunoelectron; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000145:classification / Q000378:metabolism / Q000652:urine*; D019151:Peptide Library; D011506:Proteins / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D011507:Proteinuria / Q000652:urine*; D040901:Proteomics / Q000379:methods*; D053719:Tandem Mass Spectrometry; D014357:Trypsin / Q000378:metabolism
chemical_list: D010446:Peptide Fragments; D019151:Peptide Library; D011506:Proteins; D014357:Trypsin