title: Comparison of zopiclone concentrations in oral fluid sampled with Intercept(®) oral specimen collection device and Statsure Saliva Sampler™ and concentrations in blood.
abstract: A clinical study of zopiclone was performed using doses of 5 and 10 mg. Samples of oral fluid were collected using the Statsure and Intercept devices, and blood samples were collected simultaneously. Concentrations of zopiclone in samples of oral fluid and blood were determined with liquid chromatography-mass spectrometry, and concentrations in undiluted oral fluid were calculated. The concentrations of zopiclone in oral fluid were generally higher when using the Intercept compared to the Statsure device; the median oral fluid/whole blood concentration ratios were 3.8 (range 1.5-15.9) and 1.9 (range 1.2-4.6), respectively (n = 21). The correlation between zopiclone concentrations in oral fluid collected with the two devices was fairly poor, r(2) = 0.35. The results indicate that the type of sampling device may significantly affect the analytical result for zopiclone in sampled oral fluid.
authors.affiliation: Norwegian Institute of Public Health, Division of Forensic Toxicology and Drug Abuse, P.O. Box 4404 Nydalen, NO-0403 Oslo, Norway. Hallvard.Gjerde@fhi.no
authors.forename: Hallvard
authors.initials: H
authors.lastname: Gjerde
authors.forename: Elisabeth L
authors.initials: EL
authors.lastname: Øiestad
authors.forename: Åse Marit L
authors.initials: ÅM
authors.lastname: Øiestad
authors.forename: Marit
authors.initials: M
authors.lastname: Langødegård
authors.forename: Ingebjørg
authors.initials: I
authors.lastname: Gustavsen
authors.forename: Knut
authors.initials: K
authors.lastname: Hjelmeland
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Bernard
authors.forename: Asbjørg S
authors.initials: AS
authors.lastname: Christophersen
mesh_terms: D000465:Algorithms; D053961:Azabicyclo Compounds / Q000032:analysis* / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid; D018592:Cross-Over Studies; D004305:Dose-Response Relationship, Drug; D004311:Double-Blind Method; D006801:Humans; D006993:Hypnotics and Sedatives / Q000032:analysis* / Q000097:blood* / Q000493:pharmacokinetics; D057230:Limit of Detection; D010879:Piperazines / Q000032:analysis* / Q000097:blood* / Q000493:pharmacokinetics; D015203:Reproducibility of Results; D012463:Saliva / Q000737:chemistry*; D013048:Specimen Handling / Q000295:instrumentation*; D053719:Tandem Mass Spectrometry
chemical_list: D053961:Azabicyclo Compounds; D006993:Hypnotics and Sedatives; D010879:Piperazines; C515050:zopiclone