title: Distribution of human rotavirus G and P genotypes in a hospital setting from Northern India.
abstract: Rotavirus gastroenteritis is a major cause of severe dehydrating diarrhea in children worldwide. Rotavirus G and P genotyping is essential for epidemiological surveillance and for better formulation of candidate rotavirus vaccines. Out of 862 diarrheal stool samples collected from hospitalized children aged < 2 years during February 2005 - March 2007, 318 (36.9%) were positive for rotavirus by ELISA. G and P genotyping was performed on 100 randomly selected positive samples using a seminested multiplex RT-PCR assay. The result of G genotyping indicates G1 (60%) was the most predominant VP7 type, followed by G2 (16%), G9 (8%) and G3 (3%). Two cases of G12 genotype were also observed. P genotypes identified were P[8] (40%) followed by P[4] (26%) and P[6] (17%). The most common G-P combinations were G1P[8] (26%), followed by G1P[4] and G1P[6]. Mixed infection involved 28% of strains. In this study the G1 and P[8] genotypes were the leading G and P types. Two cases with G12 genotype were also observed during the study.
authors.affiliation: Department of Microbiology, Maulana Azad Medical College and Associated Lok Nayak Hospital, New Delhi, India. anitachakravarti@gmail.com
authors.forename: Anita
authors.initials: A
authors.lastname: Chakravarti
authors.forename: Mayank Singh
authors.initials: MS
authors.lastname: Chauhan
authors.forename: Anju
authors.initials: A
authors.lastname: Sharma
authors.forename: Vikas
authors.initials: V
authors.lastname: Verma
mesh_terms: D004587:Electrophoresis, Agar Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000821:virology; D005260:Female; D005838:Genotype; D006761:Hospitals; D006801:Humans; D007194:India / Q000453:epidemiology; D007223:Infant; D008297:Male; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012401:Rotavirus / Q000235:genetics*; D012400:Rotavirus Infections / Q000453:epidemiology / Q000235:genetics* / Q000821:virology*