title: Incorporation of chlorinated analogues of aliphatic amino acids during cell-free protein synthesis.
abstract: 3-Chloro-Abu and 4-chloro-Nva are biosynthetically incorporated into E. coli peptidyl-Pro cis-trans isomerase B, as substitutes for Val and Leu, respectively. The extent of incorporation is up to ~90%, and substituted protein is catalytically active. By contrast, 4-chloro-Val is not an effective replacement for Ile.
authors.affiliation: ARC Centre of Excellence for Free Radical Chemistry and Biotechnology, Research School of Chemistry, Australian National University, Canberra ACT 0200, Australia.
authors.forename: Dannon J
authors.initials: DJ
authors.lastname: Stigers
authors.forename: Zachary I
authors.initials: ZI
authors.lastname: Watts
authors.forename: James E
authors.initials: JE
authors.lastname: Hennessy
authors.forename: Hye-Kyung
authors.initials: HK
authors.lastname: Kim
authors.forename: Romeo
authors.initials: R
authors.lastname: Martini
authors.forename: Matthew C
authors.initials: MC
authors.lastname: Taylor
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Ozawa
authors.forename: Jeffrey W
authors.initials: JW
authors.lastname: Keillor
authors.forename: Nicholas E
authors.initials: NE
authors.lastname: Dixon
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Easton
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry*; D055162:Biocatalysis; D020134:Catalytic Domain; D002474:Cell-Free System; D002713:Chlorine / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology*; D005227:Fatty Acids / Q000737:chemistry*; D007930:Leucine / Q000737:chemistry / Q000378:metabolism; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D019696:Peptidylprolyl Isomerase / Q000096:biosynthesis* / Q000737:chemistry; D014633:Valine / Q000737:chemistry / Q000378:metabolism
chemical_list: D000596:Amino Acids; D005227:Fatty Acids; D002713:Chlorine; D019696:Peptidylprolyl Isomerase; D007930:Leucine; D014633:Valine