title: First molecular identification of Sarcocystis miescheriana (Protozoa, Apicomplexa) from wild boar (Sus scrofa) in Iran.
abstract: Sarcocystis isolate obtained from the thigh muscle of a wild boar (Sus scrofa), captured from Gilan Province, northern Iran, was subjected to molecular analysis. Genomic DNA was obtained using a DNA extraction tissue kit and Polymerase chain reaction (PCR) for amplification of the 18S ribosomal DNA region yielded an 842 bp DNA band on agarose gel. Analysis of DNA sequencing by BLAST confirmed the isolate as Sarcocystis miescheriana and the sequence was deposited in GenBank by Accession No. GU395554. This is the first molecular identification of an isolate of S. miescheriana in Iran.
authors.affiliation: Department of Medical Parasitology & Mycology, School of Public Health and National Institute of Health Research, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Eshrat Beigom
authors.initials: EB
authors.lastname: Kia
authors.forename: Hossein
authors.initials: H
authors.lastname: Mirhendi
authors.forename: Mostafa
authors.initials: M
authors.lastname: Rezaeian
authors.forename: Farzaneh
authors.initials: F
authors.lastname: Zahabiun
authors.forename: Mitra
authors.initials: M
authors.lastname: Sharbatkhori
mesh_terms: D000818:Animals; D001483:Base Sequence; D016054:DNA, Protozoan / Q000737:chemistry; D004275:DNA, Ribosomal / Q000737:chemistry; D007492:Iran; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000469:parasitology; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012522:Sarcocystis / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D012523:Sarcocystosis / Q000469:parasitology / Q000662:veterinary*; D016415:Sequence Alignment / Q000662:veterinary; D034421:Sus scrofa / Q000469:parasitology*; D013552:Swine; D013553:Swine Diseases / Q000469:parasitology*; D013848:Thigh / Q000469:parasitology
chemical_list: D016054:DNA, Protozoan; D004275:DNA, Ribosomal