title: Current trends in the structure-activity relationships of sialyltransferases.
abstract: Sialyltransferases (STs) represent an important group of enzymes that transfer N-acetylneuraminic acid (Neu5Ac) from cytidine monophosphate-Neu5Ac to various acceptor substrates. In higher animals, sialylated oligosaccharide structures play crucial roles in many biological processes but also in diseases, notably in microbial infection and cancer. Cell surface sialic acids have also been found in a few microorganisms, mainly pathogenic bacteria, and their presence is often associated with virulence. STs are distributed into five different families in the CAZy database (http://www.cazy.org/). On the basis of crystallographic data available for three ST families and fold recognition analysis for the two other families, STs can be grouped into two structural superfamilies that represent variations of the canonical glycosyltransferase (GT-A and GT-B) folds. These two superfamilies differ in the nature of their active site residues, notably the catalytic base (a histidine or an aspartate residue). The observed structural and functional differences strongly suggest that these two structural superfamilies have evolved independently.
authors.affiliation: CERMAV-CNRS, Grenoble University, Grenoble, France.
authors.forename: Magali
authors.initials: M
authors.lastname: Audry
authors.forename: Charlotte
authors.initials: C
authors.lastname: Jeanneau
authors.forename: Anne
authors.initials: A
authors.lastname: Imberty
authors.forename: Anne
authors.initials: A
authors.lastname: Harduin-Lepers
authors.forename: Philippe
authors.initials: P
authors.lastname: Delannoy
authors.forename: Christelle
authors.initials: C
authors.lastname: Breton
mesh_terms: D000818:Animals; D055162:Biocatalysis; D006801:Humans; D008958:Models, Molecular; D019158:N-Acetylneuraminic Acid / Q000737:chemistry / Q000378:metabolism; D017510:Protein Folding; D012799:Sialyltransferases / Q000737:chemistry* / Q000378:metabolism*; D013329:Structure-Activity Relationship
chemical_list: D012799:Sialyltransferases; D019158:N-Acetylneuraminic Acid