title: Quantitative mass spectrometry reveals the epigenome as a target of arsenic.
abstract: Recent studies reveal that posttranslational modifications on chromatin proteins, especially histones, organize genomic DNA and mediate various cellular responses to environmental influences. Quantitative mass spectrometric analysis is a powerful approach to reveal these dynamic events on chromatin in a systematic manner. Here, the effects of arsenic exposure on histone epigenetic state were investigated in human UROtsa cells, and a reduction in acetylation level on several histone H3 and H4 lysine residues was detected. Furthermore, MYST1 was shown to be the major histone acetyltransferase for H4 Lys16 and protect UROtsa cells from arsenic toxicity.
authors.affiliation: Department of Molecular, Cellular & Biomedical Sciences, University of New Hampshire, Durham, NH 03824, USA.
authors.forename: Feixia
authors.initials: F
authors.lastname: Chu
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Ren
authors.forename: Amanda
authors.initials: A
authors.lastname: Chasse
authors.forename: Taylor
authors.initials: T
authors.lastname: Hickman
authors.forename: Luoping
authors.initials: L
authors.lastname: Zhang
authors.forename: Jessica
authors.initials: J
authors.lastname: Yuh
authors.forename: Martyn T
authors.initials: MT
authors.lastname: Smith
authors.forename: Alma L
authors.initials: AL
authors.lastname: Burlingame
mesh_terms: D000107:Acetylation; D001151:Arsenic / Q000633:toxicity*; D002460:Cell Line; D057890:Epigenomics; D015894:Genome, Human; D006657:Histones / Q000378:metabolism; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*
chemical_list: D006657:Histones; D001151:Arsenic