title: Inhibition of adhesion of Neisseria meningitidis to human epithelial cells by berry juice polyphenolic fractions.
abstract: The adhesion of pathogens to host tissues is the requirement for the initiation of the majority of infectious diseases. It was shown recently that the binding of Neisseria meningitidis pili to immobilized human epithelial cells is inhibited by molecular size fractions (10-100 kDa) of berry juices. Additionally, the isolated meningococcal pili bound to polyphenolic fractions of berry juices. The present study investigated the antiadhesive effects of berry juice polyphenolics against living meningococcal bacteria in a human epithelial cell culture model. The ability of bilberry, cranberry, crowberry and lingonberry juice polyphenolic fractions to inhibit the attachment of N. meningitidis bacteria to HEC-1B human epithelial cells in a cell culture model was examined. The antibacterial effect of the fractions was tested using a microtiter broth microdilution assay. The most effective adhesion inhibition of 75% was achieved with cranberry juice polyphenolic fraction followed by crowberry (63%), bilberry (63%) and lingonberry (57%) juice polyphenolic fractions. Bacterial survival rates after incubation with the fractions varied between 75-100%. The present results suggest berry juice polyphenols as inhibitors of adherence of N. meningitidis. Thus the binding of meningococci to berry juice polyphenols might be protective for the host against the infection.
authors.affiliation: School of Pharmacy, Faculty of Health Sciences, University of Eastern Finland, Kuopio, Finland. marko.toivanen@uef.fi
authors.forename: Marko
authors.initials: M
authors.lastname: Toivanen
authors.forename: Sanna
authors.initials: S
authors.lastname: Huttunen
authors.forename: Seppo
authors.initials: S
authors.lastname: Lapinjoki
authors.forename: Carina
authors.initials: C
authors.lastname: Tikkanen-Kaukanen
mesh_terms: D000872:Anthocyanins / Q000627:therapeutic use*; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001422:Bacterial Adhesion / Q000187:drug effects*; D002460:Cell Line; D004847:Epithelial Cells / Q000187:drug effects / Q000382:microbiology; D029791:Ericaceae / Q000737:chemistry*; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D009345:Neisseria meningitidis / Q000187:drug effects* / Q000502:physiology; D010636:Phenols / Q000737:chemistry / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology*; D059808:Polyphenols; D029796:Vaccinium / Q000737:chemistry; D029800:Vaccinium myrtillus
chemical_list: D000872:Anthocyanins; D000900:Anti-Bacterial Agents; D005419:Flavonoids; D010636:Phenols; D010936:Plant Extracts; D059808:Polyphenols; C073305:Vaccinium myrtillus extract