title: Identification and partial characterization of juvenile hormone esterase from cotton pest Dysdercus cingulatus.
abstract: Juvenile hormone esterase (JHE), a selective enzyme that hydrolyzes the methyl ester of insect juvenile hormone plays an important role in regulating metamorphosis in nymphs as well as reproduction in adults. Studies on JH degradation provide insight into the possibilities of physiological disruption in the insects. In the present study, the JH degrading enzyme, JHE from the cotton pest Dysdercus cingulatus (Heteroptera) is characterized. Electrophoretic analysis of haemolymph during various developmental stages showed the JHE bands prominent only on the final day of 5th instar nymph, and the esterase substrate specificity confirmed the presence of JHE isoforms. In an attempt to clone cDNA of JHE gene from the final instar nymphs, mRNA isolated from fat bodies was coupled with JHE gene-specific primers and the cDNA was synthesized using RT-PCR. The PCR amplified cDNA showed the presence of JHE isoforms in D. cingulatus.
authors.affiliation: Department of Zoology, University of Kerala, Kariavattom, Thiruvanathapuram 695581, Kerala, India. gayathri_elayidam@yahoo.com
authors.forename: U Gayathri
authors.initials: UG
authors.lastname: Elayidam
authors.forename: D
authors.initials: D
authors.lastname: Muraleedharen
mesh_terms: D000818:Animals; D001483:Base Sequence; D002265:Carboxylic Ester Hydrolases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D020062:Heteroptera / Q000201:enzymology*; D008970:Molecular Weight; D016133:Polymerase Chain Reaction; D013379:Substrate Specificity
chemical_list: D017931:DNA Primers; D002265:Carboxylic Ester Hydrolases; C038003:juvenile hormone esterase