title: Toll-like receptor 3 polymorphism rs3775291 is not associated with choroidal neovascularization or polypoidal choroidal vasculopathy in Chinese subjects.
abstract: OBJECTIVE
  Age-related macular degeneration (AMD) is a leading cause of visual impairment. A single-nucleotide polymorphism (SNP; rs3775291) in the Toll-like receptor 3 (TLR3) gene has recently been implicated in the pathogenesis of AMD in Caucasian populations. The aim of this study was to examine this association in Chinese persons with choroidal neovascularization (CNV) secondary to AMD and polypoidal choroidal vasculopathy (PCV).
  
  
  METHODS
  This was an observational cross-sectional study in Singapore. Study subjects were of Chinese ethnicity and included patients with exudative maculopathy and normal control subjects. The diagnoses of CNV and PCV were made based on fundus examination, fluorescein angiography and indocyanine green angiography findings. Genomic DNA was extracted, and genotypes were determined by bidirectional DNA sequencing. We compared the allele and genotype frequencies between subjects with CNV and PCV with controls using the software PLINK.
  
  
  RESULTS
  A total of 246 subjects with exudative maculopathy (consisting of 126 with CNV and 120 with PCV) and 274 normal control subjects were recruited. The distribution of rs3775291 SNP genotypes for CNV and PCV was not significantly different from that for normal controls.
  
  
  CONCLUSIONS
  This study indicates that the TLR3 rs3775291 gene polymorphism is not associated with CNV and PCV in Singaporean Chinese patients.
authors.affiliation: Singapore Eye Research Institute, National University of Singapore, Singapore.
authors.forename: Chelvin C A
authors.initials: CC
authors.lastname: Sng
authors.forename: Peter D
authors.initials: PD
authors.lastname: Cackett
authors.forename: Ian Y
authors.initials: IY
authors.lastname: Yeo
authors.forename: Anbupalam
authors.initials: A
authors.lastname: Thalamuthu
authors.forename: Anandalakshmi
authors.initials: A
authors.lastname: Venkatraman
authors.forename: Divya
authors.initials: D
authors.lastname: Venkataraman
authors.forename: Adrian H
authors.initials: AH
authors.lastname: Koh
authors.forename: E-Shyong
authors.initials: ES
authors.lastname: Tai
authors.forename: Tien Y
authors.initials: TY
authors.lastname: Wong
authors.forename: Tin
authors.initials: T
authors.lastname: Aung
authors.forename: Eranga N
authors.initials: EN
authors.lastname: Vithana
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D044466:Asian People / Q000235:genetics*; D002829:Choroid / Q000098:blood supply*; D015862:Choroid Diseases / Q000235:genetics*; D020256:Choroidal Neovascularization / Q000235:genetics*; D003430:Cross-Sectional Studies; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D016491:Peripheral Vascular Diseases / Q000235:genetics*; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D012846:Singapore / Q000453:epidemiology; D051196:Toll-Like Receptor 3 / Q000235:genetics*
chemical_list: C495344:TLR3 protein, human; D051196:Toll-Like Receptor 3