title: ECHO-LNA conjugates: hybridization-sensitive fluorescence and its application to fluorescent detection of various RNA strands.
abstract: Hybridization-sensitive fluorescent DNA probes containing the nucleotide units of locked nucleic acid (LNA) have been developed. Exciton-controlled hybridization-sensitive fluorescent oligonucleotide (ECHO) probes that incorporated LNA nucleotides achieved high thermostability of the hybrid with target RNA strands. The appropriately designed ECHO-LNA chimeric probes exhibited an effective on-off switching property of fluorescence depending on hybridization with RNA and facilitated fluorescent detection of the TAR RNA strand forming a hairpin structure and distinction of one base difference in PLAC4 RNA sequence.
authors.affiliation: RIKEN Advanced Science Institute, Wako, Saitama 351-0198, Japan.
authors.forename: Kaori
authors.initials: K
authors.lastname: Sugizaki
authors.forename: Akimitsu
authors.initials: A
authors.lastname: Okamoto
mesh_terms: D001483:Base Sequence; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry; D015342:DNA Probes / Q000138:chemical synthesis* / Q000378:metabolism; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000378:metabolism; D016325:HIV Long Terminal Repeat; D006801:Humans; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization / Q000379:methods*; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000378:metabolism; D012313:RNA / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D020871:RNA Stability; D013050:Spectrometry, Fluorescence; D044366:Transition Temperature
chemical_list: D015342:DNA Probes; D005456:Fluorescent Dyes; D009841:Oligonucleotides; D012313:RNA; D004247:DNA