title: In vivo sarcomere imaging and fiber tension measurements.
abstract: Current work demonstrates a new method and a new device for simultaneous in vivo measurements of sarcomere imaging and fiber tension. Sarcomere, a building block of muscles, plays a critical role in muscle contraction. In the pathological conditions, the biomechanical properties of sarcomere and muscle fibers may be altered significantly. We developed a novel methodology by combining the optical image capture system with force measurement which allowed us to investigate the muscle cell properties in vivo under monitored/controlled tension. The methodology was validated in an in vivo rat model as well as using an in vitro corroboration setup. The in vivo evaluation provides us a powerful tool to investigate pathological changes of fibers/sarcomeres and help determine proper treatment.
authors.affiliation: Rehabilitation Institute of Chicago, IL 60611, USA. winnie@northwestern.edu
authors.forename: Yi-Ning
authors.initials: YN
authors.lastname: Wu
authors.forename: Yupeng
authors.initials: Y
authors.lastname: Ren
authors.forename: Li-Qun
authors.initials: LQ
authors.lastname: Zhang
mesh_terms: D000465:Algorithms; D000818:Animals; D001696:Biomechanical Phenomena; D001698:Biomedical Engineering / Q000379:methods; D008297:Male; D015233:Models, Statistical; D009119:Muscle Contraction; D018485:Muscle Fibers, Skeletal / Q000473:pathology; D009132:Muscles / Q000473:pathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012518:Sarcomeres / Q000473:pathology*; D012815:Signal Processing, Computer-Assisted