title: Acetylation of H4K12 in porcine oocytes during in vitro aging: potential role of ooplasmic reactive oxygen species.
abstract: Deterioration in the quality of mammalian mature oocytes during metaphase-II (M-II) arrest is called "oocyte aging". Although histone acetylation may affect the progression of aging in murine oocytes, the mechanism is unknown. The objective was to determine the role of ooplasmic reactive oxygen species (ROS) in acetylation of histone H4 at lysine 12 (acH4K12) in porcine aged oocytes in vitro. Based on immunostaining with a specific antibody, acetylation of H4K12 in porcine oocytes increased during in vitro aging, which coincided with changing patterns of ooplasmic ROS content. Furthermore, both hydrogen peroxide (H(2)O(2)), and the mitochondrial membrane potential disrupter, carbonyl cyanide 3-chlorophenylhydrazone (CCCP), which can moderately elevate oocyte ROS content, significantly increased acetylation levels of H4K12 in porcine oocytes. It was noteworthy that acetylation in the CCCP group was decreased when ROS was counteracted by cysteine, a common antioxidant. In addition, the intracellular mRNA abundance of acetyltransferase gene HAT1 in aged and H(2)O(2) treated oocytes was higher than in M-II phase oocytes, suggesting that HAT1 was involved in this reaction. After parthenogenetic activation, a lower proportion of oocytes developed to the blastocyst stage after CCCP or H(2)O(2) treatment when compared with M-II phase oocytes (20 and 0% for CCCP and H(2)O(2) groups, respectively, versus 42% for the M-II group, P < 0.05). In conclusion, elevated levels of H4K12 acetylation were attributed to increased ooplasmic ROS content during porcine oocyte aging in vitro.
authors.affiliation: Laboratory of Animal Embryonic Biotechnology, College of Animal Science and Technology, China Agricultural University, Beijing, People's Republic of China.
authors.forename: Mao-Sheng
authors.initials: MS
authors.lastname: Cui
authors.forename: Xian-Long
authors.initials: XL
authors.lastname: Wang
authors.forename: Da-Wei
authors.initials: DW
authors.lastname: Tang
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.forename: Shen-Ming
authors.initials: SM
authors.lastname: Zeng
mesh_terms: D000107:Acetylation / Q000187:drug effects; D000818:Animals; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology; D018929:Cell Culture Techniques; D003545:Cysteine / Q000494:pharmacology; D047108:Embryonic Development / Q000187:drug effects; D057890:Epigenomics; D005260:Female; D051548:Histone Acetyltransferases / Q000235:genetics / Q000378:metabolism; D006657:Histones / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D009865:Oocytes / Q000187:drug effects / Q000254:growth & development*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013552:Swine / Q000235:genetics*
chemical_list: D006657:Histones; D017382:Reactive Oxygen Species; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D006861:Hydrogen Peroxide; D051548:Histone Acetyltransferases; C110880:histone acetyltransferase type B complex; D003545:Cysteine