title: Verticillium dahliae alters Pseudomonas spp. populations and HCN gene expression in the rhizosphere of strawberry.
abstract: The production of hydrogen cyanide (HCN) by beneficial root-associated bacteria is an important mechanism for the biological control of plant pathogens. However, little is known about the biotic factors affecting HCN gene expression in the rhizosphere of plants. In this study, real-time reverse transcription PCR (qRT-PCR) assays were developed to investigate the effect of the plant pathogen Verticillium dahliae on hcnC (encoding for HCN biosynthesis) gene expression in Pseudomonas sp. LBUM300. Strawberry plants were inoculated with Pseudomonas sp. LBUM300 and (or) V. dahliae and grown in pots filled with nonsterilized field soil. RNA was extracted from rhizosphere soil sampled at 0, 15, 30, and 45 days following inoculation with V. dahliae and used for qRT-PCR analyses. Populations of V. dahliae and Pseudomonas sp. LBUM300 were also monitored using a culture-independent qPCR approach. hcnC expression was detected at all sampling dates. The presence of V. dahliae had a significant stimulation effect on hcnC gene expression and also increased the population of Pseudomonas sp. LBUM300. However, the V. dahliae population was not altered by the presence of Pseudomonas sp. LBUM300. To our knowledge, this study is the first to evaluate the effect of a plant pathogen on HCN gene expression in the rhizosphere soil.
authors.affiliation: Université de Moncton, Department of Biology, Moncton, NB E1A 3E9, Canada.
authors.forename: Nadine J
authors.initials: NJ
authors.lastname: DeCoste
authors.forename: Vijay J
authors.initials: VJ
authors.lastname: Gadkar
authors.forename: Martin
authors.initials: M
authors.lastname: Filion
mesh_terms: D031985:Fragaria / Q000382:microbiology*; D015870:Gene Expression; D006856:Hydrogen Cyanide / Q000378:metabolism*; D010935:Plant Diseases / Q000382:microbiology; D018517:Plant Roots / Q000382:microbiology; D011549:Pseudomonas / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D058441:Rhizosphere; D020172:Verticillium / Q000502:physiology*
chemical_list: D006856:Hydrogen Cyanide