title: Fluorescence-surface enhanced Raman scattering co-functionalized gold nanorods as near-infrared probes for purely optical in vivo imaging.
abstract: Gold nanorods (GNRs) have been widely used for bio-imaging. However, GNRs assisted optical in vivo deep tissue imaging is severely restricted due to signal attenuation, low contrast, complex process or low real-timing. To overcome these problems, we functionalized GNRs with both near-infrared (NIR) fluorescence and surface enhanced Raman scattering (SERS) and utilized these co-functionalized GNRs for purely optical in vivo imaging of live mice. Our proposed technology has the combined advantages of high real-timing, high imaging contrast and deep detection ability. The distribution and excretion of intravenously injected GNRs in deep tissues of live mice were observed in vivo for the first time through purely optical imaging. We also demonstrated successfully in vivo biomedical applications of the co-functionalized GNRs to sentinel lymph node (SLN) mapping and tumor targeting of mice. The present technology has great future potentials for disease diagnosis and clinical therapies.
authors.affiliation: Centre for Optical and Electromagnetic Research, State Key Laboratory of Modern Optical Instrumentations, Joint Research Center of Photonics of the Royal Institute of Technology (Sweden), Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Qian
authors.forename: Li
authors.initials: L
authors.lastname: Jiang
authors.forename: Fuhong
authors.initials: F
authors.lastname: Cai
authors.forename: Dan
authors.initials: D
authors.lastname: Wang
authors.forename: Sailing
authors.initials: S
authors.lastname: He
mesh_terms: D000818:Animals; D001943:Breast Neoplasms / Q000473:pathology; D045744:Cell Line, Tumor; D003952:Diagnostic Imaging / Q000379:methods*; D005260:Female; D005453:Fluorescence; D006046:Gold / Q000737:chemistry*; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D043942:Nanotubes / Q000737:chemistry*; D013059:Spectrum Analysis, Raman / Q000379:methods*
chemical_list: D006046:Gold