title: Structural study of acetogenins by tandem mass spectrometry under high and low collision energy.
abstract: Collision-induced dissociation experiments of seven annonaceous acetogenins were carried out under high and low collision energy conditions. Each compound was studied as protonated or deprotonated and lithium- or sodium- cationized molecules, using ElectroSpray Ionisation (ESI) with a hybrid linear trap/orbitrap mass spectrometer (LTQ-Orbitrap®). The same ion species were studied with a Matrix-Assisted Laser Desorption Ionisation (MALDI) tandem mass spectrometer in a high collision energy regime (1 or 2 keV). Although each of the techniques showed some limitations in the detection of functional groups, unambiguous structural identification of the acetogenins was obtained. MALDI ToF-ToF has the advantage over ESI-based methods to provide mass spectra rich in informative fragments which allows the confirmation of some functional groups position. By contrast, ESI-LTQ-Orbitrap® analysis has the advantage over MALDI that the mass spectra are relatively simple with only fragments close to the functional groups. However, this technique needs to carry out experiments both in negative and positive ionization modes. Copyri
authors.affiliation: Équipe de Spectrométrie de Masse, Centre de recherche de Gif, Institut de Chimie des Substances Naturelles, CNRS, Avenue de la Terrasse, 91198 Gif-sur-Yvette Cedex, France.
authors.forename: Julie
authors.initials: J
authors.lastname: Allegrand
authors.forename: David
authors.initials: D
authors.lastname: Touboul
authors.forename: Isabelle
authors.initials: I
authors.lastname: Schmitz-Afonso
authors.forename: Vincent
authors.initials: V
authors.lastname: Guérineau
authors.forename: Alexandre
authors.initials: A
authors.lastname: Giuliani
authors.forename: Jessica
authors.initials: J
authors.lastname: Le Ven
authors.forename: Pierre
authors.initials: P
authors.lastname: Champy
authors.forename: Olivier
authors.initials: O
authors.lastname: Laprévote
mesh_terms: D054378:Acetogenins / Q000737:chemistry*; D007477:Ions / Q000737:chemistry; D008094:Lithium / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D054378:Acetogenins; D007477:Ions; D008094:Lithium