title: [Clinical implications of HLA-G protein expression in acute leukemia].
abstract: OBJECTIVE
  To detect the expression of membrane-bound HLA-G (mHLA-G) and serum HLA-G (sHLA-G) in acute leukemia patients and investigate the correlation between HLA-G expression and the occurrence and development of acute leukemia.
  
  
  METHODS
  Enzyme-linked immunosorbent assay and flow cytometry were used to detect the expression levels of sHLA-G and mHLA-G in 40 newly diagnosed leukemia cases, 10 refractory and relapsed leukemia cases, and 30 leukemia cases receiving chemotherapy. Ten normal individuals served as the normal control group.
  
  
  RESULTS
  The mean serum level of sHLA-G in normal individuals was 5.87±2.07 ng/ml, as compared to 10.05±6.58 ng/ml in newly diagnosed leukemia patients and 12.32±5.85 ng/ml in refractory and relapsed cases. The mean level of mHLA-G in normal individuals, newly diagnosed cases, and refractory and relapsed cases were (0.29±0.20)%, (0.60±0.44)%, and (0.77±0.41)%, respectively. The mean levels of sHLA-G and mHLA-G were significantly higher in the newly diagnosed cases than that in the normal controls (P<0.05), and significantly higher in patients before chemotherapy than in those with complete remission after chemotherapy (P<0.05).
  
  
  CONCLUSIONS
  HLA-G expression levels might influence the treatment outcomes and can serve as a prognostic factor for acute leukemia.
authors.affiliation: Department of Hematology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Li
authors.forename: Xing-hui
authors.initials: XH
authors.lastname: Cao
authors.forename: Can
authors.initials: C
authors.lastname: Liu
authors.forename: Xiu-li
authors.initials: XL
authors.lastname: Wu
authors.forename: Yi-wen
authors.initials: YW
authors.lastname: Ling
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.forename: Ru
authors.initials: R
authors.lastname: Feng
authors.forename: Qi-fa
authors.initials: QF
authors.lastname: Liu
mesh_terms: D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D005260:Female; D059951:HLA-G Antigens / Q000097:blood / Q000378:metabolism*; D006801:Humans; D007938:Leukemia / Q000378:metabolism* / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D055815:Young Adult
chemical_list: D059951:HLA-G Antigens