title: Learning cellular texture features in microscopic cancer cell images for automated cell-detection.
abstract: In this paper we present a new approach for automated cell detection in single frames of 2D microscopic phase contrast images of cancer cells which is based on learning cellular texture features. The main challenge addressed in this paper is to deal with clusters of cells where each cell has a rather complex appearance composed of sub-regions with different texture features. Our approach works on two different levels of abstraction. First, we apply statistical learning to learn 6 different types of different local cellular texture features, classify each pixel according to them and we obtain an image partition composed of 6 different pixel categories. Based on this partitioned image we decide in a second step if pre-selected seeds belong to the same cell or not. Experimental results show the high accuracy of the proposed method and especially average precision above 95%.
authors.affiliation: Biomedical Data Analysis Group, Software Competence Center Hagenberg GmbH, Softwarepark 21, A-4232, Austria. tomas.kazmar@scch.at
authors.forename: Tomas
authors.initials: T
authors.lastname: Kazmar
authors.forename: Matej
authors.initials: M
authors.lastname: Smid
authors.forename: Margit
authors.initials: M
authors.lastname: Fuchs
authors.forename: Birgit
authors.initials: B
authors.lastname: Luber
authors.forename: Julian
authors.initials: J
authors.lastname: Mattes
mesh_terms: D000465:Algorithms; D002467:Cell Nucleus / Q000473:pathology; D003584:Cytological Techniques / Q000379:methods*; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008858:Microscopy, Phase-Contrast / Q000379:methods*; D009369:Neoplasms / Q000473:pathology*; D010363:Pattern Recognition, Automated / Q000379:methods*