title: An approach for differentiating uniform glutamatergic neurons from mouse embryonic stem cells.
abstract: Much effort is being marshaled to generate uniform neuronal populations from embryonic stem (ES) cells, but a completely reliable method has yet to be developed. Here we modified and established a method that brings us closer to this goal. By examining many parameters, we found that the optimal timing of applying a freshly made trypsin/EDTA (ethylenediaminetetraacetic acid) solution to dissociate embryoid bodies determines the success of the outcome. Analyses demonstrated that with this approach, more than 87% of cells differentiated into glutamatergic neurons. Hence, these uniform neurons that were differentiated from ES cells provide an ideal cellular model for many aspects of research.
authors.affiliation: Department of Life Science, National Taiwan Normal University, Taipei 116, Taiwan.
authors.forename: Jen-Hua
authors.initials: JH
authors.lastname: Chuang
authors.forename: Li-Chu
authors.initials: LC
authors.lastname: Tung
authors.forename: Guey-Jen
authors.initials: GJ
authors.lastname: Lee-Chen
authors.forename: Yi
authors.initials: Y
authors.lastname: Yin
authors.forename: Yenshou
authors.initials: Y
authors.lastname: Lin
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D003584:Cytological Techniques / Q000379:methods*; D058732:Embryoid Bodies / Q000166:cytology; D053595:Embryonic Stem Cells / Q000166:cytology*; D018698:Glutamic Acid / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D051379:Mice; D009474:Neurons / Q000166:cytology* / Q000378:metabolism*
chemical_list: D018698:Glutamic Acid