title: Depigmenting mechanism of NSAIDs on B16F1 melanoma cells.
abstract: The aim of the present work was to clarify the anti-melanogenic mechanism of non-steroidal anti-inflammatory drugs (NSAIDs). Mefenamic acid, diclofenac, and nimesulide were used in this study, and these drugs inhibit melanin synthesis in B16F1 melanoma cells. To elucidate the anti-melanogenic mechanism of NSAIDs, we performed western blotting analysis for melanogenic proteins, such as tyrosinase, TRP-1, and TRP-2. All NSAIDs used in this study inhibited tyrosinase protein level. Semi-quantitative RT-PCR analysis showed that the depigmentation effect of mefenamic acid and nimesulide might be due to the inhibition of tyrosinase gene transcription. These results indicate that NSAIDs inhibit α-MSH-enhanced melanin synthesis, and are candidate anti-melanogenic agents since they might be effective in hyperpigmentation disorders.
authors.affiliation: Department of Life Science, College of Agriculture, Tamagawa University, Machida, Tokyo, Japan. kzsato@agr.tamagawa.ac.jp
authors.forename: Kazuomi
authors.initials: K
authors.lastname: Sato
authors.forename: Hideki
authors.initials: H
authors.lastname: Takahashi
authors.forename: Masaru
authors.initials: M
authors.lastname: Toriyama
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D004008:Diclofenac / Q000494:pharmacology; D006801:Humans; D019746:Intramolecular Oxidoreductases / Q000235:genetics / Q000378:metabolism; D008528:Mefenamic Acid / Q000494:pharmacology; D008543:Melanins / Q000378:metabolism*; D008546:Melanoma, Experimental; D051379:Mice; D014442:Monophenol Monooxygenase / Q000235:genetics / Q000378:metabolism*; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism; D010859:Pigmentation Disorders / Q000188:drug therapy*; D012880:Skin Pigmentation / Q000187:drug effects*; D013449:Sulfonamides; D000521:alpha-MSH / Q000378:metabolism
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D008543:Melanins; D013449:Sulfonamides; D004008:Diclofenac; D008528:Mefenamic Acid; D000521:alpha-MSH; D010088:Oxidoreductases; C064056:tyrosinase-related protein-1; D014442:Monophenol Monooxygenase; D019746:Intramolecular Oxidoreductases; C042501:dopachrome isomerase; C012655:nimesulide