title: Multi scale and slice-based approach for automatic spine detection.
abstract: Dendritic spines play an essential role in the central nervous system. Recent experiments have revealed that neuron functional properties are highly correlated with the statistical and morphological changes of the dendritic spines. In this paper, we propose a new multi scale approach for detecting dendritic spines in a 2D Maximum Intensity Projection (MIP) image of the 3D neuron data stacks collected from a 2-photon laser scanning confocal microscope. The proposed method utilizes the curvilinear structure detector in conjunction with the multi scale spine detection algorithm which automatically and accurately extracts and segments the spines with variational sizes along the dendrite. In addition, a slice-based spine detection algorithm is also proposed to detect spines which are hidden from the MIP image within the dendrite area. Experimental results show that our proposed method is effective for automatic spine detection and is able to accurately segment dendrite.
authors.affiliation: Department of Mathematics, Hong Kong Baptist University, Hong Kong, China.
authors.forename: Siu-Kai
authors.initials: SK
authors.lastname: Choy
authors.forename: Kun
authors.initials: K
authors.lastname: Chen
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.forename: Matthew
authors.initials: M
authors.lastname: Baron
authors.forename: Merilee A
authors.initials: MA
authors.lastname: Teylan
authors.forename: Yong
authors.initials: Y
authors.lastname: Kim
authors.forename: Chong-Sze
authors.initials: CS
authors.lastname: Tong
authors.forename: Zhihuan
authors.initials: Z
authors.lastname: Song
authors.forename: Stephen T C
authors.initials: ST
authors.lastname: Wong
mesh_terms: D000465:Algorithms; D001185:Artificial Intelligence; D049229:Dendritic Spines / Q000648:ultrastructure*; D006801:Humans; D007089:Image Enhancement / Q000379:methods*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D018613:Microscopy, Confocal / Q000379:methods*; D010363:Pattern Recognition, Automated / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity