title: Grafted glycopolymer-based receptor mimics on polymer support for selective adhesion of bacteria.
abstract: A sugar-containing monomer (2-lactobionamidoethyl methacrylate, LAMA) was grafted on a polypropylene (PP) microfiltration membrane surface by UV-induced graft copolymerization. The degree of grafting can be controlled by variation of monomer concentration, UV irradiation time, and photoinitiator concentration. Fourier transform infrared spectroscopy and scanning electron microscopy were employed to confirm the surface modification on the membranes. The water contact angle was used to evaluate the hydrophilicity change of the membrane surface before and after modification. Bacteria capture experiments showed that the membrane could selectively bind E. faecalis while adhesion of S. maltophilia was not influenced by the functionalization of PP with grafted poly(LAMA). The adhesion of E. faecalis onto poly(LAMA) grafted membrane could be inhibited by 200 mM galactose solution; however, glucose solution showed no inhibition effect. Moreover, occupying sugar residues on the membrane surface primarily by a galactose targeting lectin, peanut agglutinin, could significantly suppress the following adhesion of E. faecalis. All these results clearly demonstrate that this poly(LAMA) grafted PP membrane can selectively capture E. faecalis and that this selection is based on the interaction between galactose side groups on grafted flexible functional polymer chains on the membrane surface and galactose binding protein on the E. faecalis cell membrane.
authors.affiliation: Lehrstuhl für Technische Chemie II, Universität Duisburg-Essen, 45117 Essen, Germany.
authors.forename: Qian
authors.initials: Q
authors.lastname: Yang
authors.forename: Martin
authors.initials: M
authors.lastname: Strathmann
authors.forename: Anna
authors.initials: A
authors.lastname: Rumpf
authors.forename: Gabriela
authors.initials: G
authors.lastname: Schaule
authors.forename: Mathias
authors.initials: M
authors.lastname: Ulbricht
mesh_terms: D001422:Bacterial Adhesion / Q000502:physiology*; D040761:Biomimetic Materials / Q000737:chemistry* / Q000378:metabolism*; D050260:Carbohydrate Metabolism / Q000502:physiology*; D013293:Enterococcus faecalis / Q000166:cytology / Q000502:physiology*; D008422:Materials Testing; D011956:Receptors, Cell Surface / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D011956:Receptors, Cell Surface