title: Star-shaped nano-conjugates of cisplatin with high drug payload.
abstract: Core-shell type star polymer bearing carboxylate functions was designed and evaluated as nanocarrier of cisplatin. The synthetic route to the star macromolecules involved the "core first" method to yield a precursor star polymer with a highly branched poly(styrene) core and poly(tert-butyl acrylate) arms. Two polymers derived from a common core of M(n) = 2400 g/mol and degrees of polymerization of the linear arms 38 and 58 were subjected to acidic hydrolysis to obtain stars with a hydrophilic and multifunctional shell. Diffusion ordered NMR spectroscopic study revealed that the two products presented single populations of stars with values of the apparent hydrodynamic radii 12.9 nm and 14.0 nm, respectively. The stars were loaded with cisplatin via ligand exchange reaction achieving remarkable high drug payload of 45% (w/w). The conjugates were stable in an aqueous solution exhibiting no precipitation for a prolonged period of time. The release profile of the platinum (II) complexes in phosphate buffered saline and RPMI-1640 liquid medium at 37 °C indicated sustained manner of drug release with no initial burst effect. In vitro cell viability study, using four human tumor cell lines proved that the conjugates exhibited lower cytotoxicity compared to the free agent. The established cellular accumulation of cisplatin indicated uptake of the nanoconjugates by the cells through endocytosis.
authors.affiliation: Centre of Polymer and Carbon Materials, Polish Academy of Sciences, 34 M Curie-Sklodowskiej 41-819 Zabrze, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Kowalczuk
authors.forename: Ekaterina
authors.initials: E
authors.lastname: Stoyanova
authors.forename: Violeta
authors.initials: V
authors.lastname: Mitova
authors.forename: Pavletta
authors.initials: P
authors.lastname: Shestakova
authors.forename: Georgi
authors.initials: G
authors.lastname: Momekov
authors.forename: Denitsa
authors.initials: D
authors.lastname: Momekova
authors.forename: Neli
authors.initials: N
authors.lastname: Koseva
mesh_terms: D000179:Acrylates / Q000138:chemical synthesis / Q000737:chemistry*; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002626:Chemistry, Pharmaceutical; D002945:Cisplatin / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D003692:Delayed-Action Preparations; D004305:Dose-Response Relationship, Drug; D004337:Drug Carriers; D004339:Drug Compounding; D004705:Endocytosis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D057927:Hydrophobic and Hydrophilic Interactions; D020128:Inhibitory Concentration 50; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D008970:Molecular Weight; D058726:Nanoconjugates; D036103:Nanotechnology; D011137:Polystyrenes / Q000138:chemical synthesis / Q000737:chemistry*; D012995:Solubility; D013678:Technology, Pharmaceutical / Q000379:methods
chemical_list: D000179:Acrylates; D000970:Antineoplastic Agents; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D058726:Nanoconjugates; D011137:Polystyrenes; C509854:poly(styrene-b-tert-butylacrylate); D002945:Cisplatin