title: [The isolation and assessment of Golgi apparatus from gastric cancer cells SGC7901].
abstract: The Golgi complex is the central organelle of the secretory pathway and has many complicate functions. The endeavours to isolate and purify the Golgi apparatus from cultured cells will benefit further investigation of Golgi. A large number of gastric cancer cells SGC7901 were cultivated in vitro, then Golgi apparatus were isolated from the cells by differential centrifugation combined with sucrose density gradient ultra-centrifugation. Its purity was characterized biochemically by enzymatic assays, morphologically by electron microscopy (EM) and neutral red supravital staining. Finally the Golgi complex was successfully fractionated from gastric cancer cells SGC7901. The first successful isolation of Golgi apparatus from gastric cancer cells SGC7901 by using ultra-centrifugation will lead to research into the function of Golgi apparatus.
authors.affiliation: Department of Pathology, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: He
authors.forename: Yongfen
authors.initials: Y
authors.lastname: Yi
authors.forename: Yanqing
authors.initials: Y
authors.lastname: Li
authors.forename: Zhong
authors.initials: Z
authors.lastname: Xiao
mesh_terms: D045744:Cell Line, Tumor; D006056:Golgi Apparatus / Q000648:ultrastructure*; D006652:Histological Techniques; D006801:Humans; D013274:Stomach Neoplasms / Q000473:pathology / Q000648:ultrastructure*