title: Prephenate aminotransferase directs plant phenylalanine biosynthesis via arogenate.
abstract: The aromatic amino acids L-phenylalanine and L-tyrosine and their plant-derived natural products are essential in human and plant metabolism and physiology. Here we identified Petunia hybrida and Arabidopsis thaliana genes encoding prephenate aminotransferases (PPA-ATs), thus completing the identification of the genes involved in phenylalanine and tyrosine biosyntheses. Biochemical and genetic characterization of enzymes showed that PPA-AT directs carbon flux from prephenate toward arogenate, making the arogenate pathway predominant in plant phenylalanine biosynthesis.
authors.affiliation: Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, Indiana, USA.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Maeda
authors.forename: Heejin
authors.initials: H
authors.lastname: Yoo
authors.forename: Natalia
authors.initials: N
authors.lastname: Dudareva
mesh_terms: D057486:Carbon Cycle / Q000502:physiology; D006801:Humans; D006836:Hydro-Lyases / Q000378:metabolism*; D010649:Phenylalanine / Q000096:biosynthesis*; D010940:Plant Proteins / Q000096:biosynthesis*; D010944:Plants / Q000201:enzymology*; D000637:Transaminases / Q000378:metabolism*; D014443:Tyrosine / Q000031:analogs & derivatives / Q000096:biosynthesis
chemical_list: D010940:Plant Proteins; D014443:Tyrosine; D010649:Phenylalanine; D000637:Transaminases; C044954:prephenate aminotransferase; D006836:Hydro-Lyases; C022437:pretyrosine dehydratase