title: Characterization of Leishmania spp. causing cutaneous leishmaniasis in Manaus, Amazonas, Brazil.
abstract: In the State of Amazonas, American tegumentary leishmaniasis is endemic and presents a wide spectrum of clinical variability due to the large diversity of circulating species in the region. Isolates from patients in Manaus and its metropolitan region were characterized using monoclonal antibodies and isoenzymes belonging to four species of the parasite: Leishmania (Viannia) guyanensis, 73% (153/209); Leishmania (Viannia) braziliensis, 14% (30/209); Leishmania (Leishmania) amazonensis, 8% (17/209); and Leishmania (Viannia) naiffii, 4% (9/209). The most prevalent species was L. (V.) guyanensis. The principal finding of this study was the important quantity of infections involving more than one parasite species, representing 14% (29/209) of the total. The findings obtained in this work regarding the parasite are further highlighted by the fact that these isolates were obtained from clinical samples collected from single lesions.
authors.affiliation: Gerencia de Leishmanioses, Funbdação de Medicina Tropical do Amazonas, Manaus, Brazil. liarccoelho@yahoo.com.br
authors.forename: Leila Ines
authors.initials: LI
authors.lastname: Camara Coelho
authors.forename: Marcilene
authors.initials: M
authors.lastname: Paes
authors.forename: Jorge Augusto
authors.initials: JA
authors.lastname: Guerra
authors.forename: Maria das Graças
authors.initials: Md
authors.lastname: Barbosa
authors.forename: Candisse
authors.initials: C
authors.lastname: Coelho
authors.forename: Bruna
authors.initials: B
authors.lastname: Lima
authors.forename: Maria Edileuza
authors.initials: ME
authors.lastname: Brito
authors.forename: Sinval Pinto
authors.initials: SP
authors.lastname: Brandão Filho
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D000953:Antigens, Protozoan / Q000276:immunology; D044963:Biopsy, Fine-Needle; D001938:Brazil / Q000453:epidemiology; D002648:Child; D002675:Child, Preschool; D004587:Electrophoresis, Agar Gel; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007223:Infant; D007527:Isoenzymes; D007891:Leishmania / Q000145:classification / Q000201:enzymology / Q000276:immunology / Q000302:isolation & purification; D016773:Leishmaniasis, Cutaneous / Q000145:classification / Q000453:epidemiology / Q000276:immunology / Q000469:parasitology*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction
chemical_list: D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan; D007527:Isoenzymes