title: Heparin/collagen multilayer as a thromboresistant and endothelial favorable coating for intravascular stent.
abstract: Endothelialization and antithrombogenicity are two key issues in stent implantation. The layer-by-layer (LbL) deposition of anticoagulant heparin and cell compatible collagen was explored to develop a multilayered coating with synergic property of antithrombogenicity and fast endothelialization. The quartz crystal microbalance with dissipation (QCM-D), UV spectrometer, spectroscopic ellipsometry, scanning electron microscopy, and confocal laser scanning microscopy investigations indicate that the LbL technique, which based on molecular assembly, provides an easy way to develop a smooth, homogenous, and stable coating onto stents. In vitro blood clotting time tests and the platelet adhesion tests show that the multilayer-modified stents present good hemocompatibility. In vitro endothelial cell (EC) culture results show that the multilayer-modified surfaces accelerate the adhesion and proliferation of ECs. These results illustrate that a stent surface coating with properties of antithrombogenicity and EC preference was obtained via heparin/collagen multilayer modification. This surface coating may have great potential in facilitating in situ endothelialization of blood contacting materials.
authors.affiliation: MOE Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, China.
authors.forename: Quankui
authors.initials: Q
authors.lastname: Lin
authors.forename: Jiajie
authors.initials: J
authors.lastname: Yan
authors.forename: Fuyu
authors.initials: F
authors.lastname: Qiu
authors.forename: Xiaoxiao
authors.initials: X
authors.lastname: Song
authors.forename: Guosheng
authors.initials: G
authors.lastname: Fu
authors.forename: Jian
authors.initials: J
authors.lastname: Ji
mesh_terms: D000925:Anticoagulants / Q000737:chemistry*; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D003094:Collagen / Q000737:chemistry*; D042783:Endothelial Cells / Q000378:metabolism* / Q000648:ultrastructure; D006493:Heparin / Q000737:chemistry*; D006801:Humans; D008422:Materials Testing; D010973:Platelet Adhesiveness; D058525:Quartz Crystal Microbalance Techniques; D015607:Stents; D013499:Surface Properties
chemical_list: D000925:Anticoagulants; D020099:Coated Materials, Biocompatible; D006493:Heparin; D003094:Collagen