title: Novel PKCs activate ERK through PKD1 in MCF-7 cells.
abstract: PKCs can have opposite effects on ERK phosphorylation. Novel (n)PKCs can inhibit ERK by phosphorylation of Raf-1, classical and atypical PKCs can activate ERK by removing an inhibitory protein from Raf-1. The aim of this work was to clarify how PMA-activated PKCs lead to ERK activation in MCF-7 cells expressing mainly nPKCs. Using chemical inhibitors and antibodies against PKCs, delivered into cells by the Chariot transfection system, we found that nPKCs activate ERK through transphosphorylation of PKD1, the blockage of which prevented PMA-stimulated ERK activation. We conclude that the nPKCs/PKD1 cascade is determinant for ERK activation by PMA in MCF-7 cells.
authors.affiliation: Department of Physiology, University of Siena, via Aldo Moro 7, Siena, Italy.
authors.forename: Claudia
authors.initials: C
authors.lastname: Torricelli
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Valacchi
authors.forename: Emanuela
authors.initials: E
authors.lastname: Maioli
mesh_terms: D015153:Blotting, Western; D002460:Cell Line; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D008954:Models, Biological; D010766:Phosphorylation; D011493:Protein Kinase C / Q000378:metabolism*; D019908:Proto-Oncogene Proteins c-raf / Q000378:metabolism; D050396:TRPP Cation Channels / Q000378:metabolism*; D014162:Transfection / Q000379:methods
chemical_list: D048049:Extracellular Signal-Regulated MAP Kinases; D011493:Protein Kinase C; D019908:Proto-Oncogene Proteins c-raf; D050396:TRPP Cation Channels