title: Efficient and direct glutathione production from raw starch using engineered Saccharomyces cerevisiae.
abstract: Glutathione is a valuable tri-peptide that is widely used in the pharmaceutical, food, and cosmetic industries. Glutathione is produced industrially by fermentation using Saccharomyces cerevisiae. We demonstrated that expression of amylase genes in glutathione-producing S. cerevisiae enables direct use of starch as a carbon source, thus eliminating the Crabtree effect that is caused by excess glucose. Consequently, cell growth and glutathione productivity were significantly improved. This approach is potentially applicable to a variety of fermentative processes for production of value-added chemicals under aerobic conditions.
authors.affiliation: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657-8501, Japan.
authors.forename: Hideyo
authors.initials: H
authors.lastname: Yoshida
authors.forename: Shogo
authors.initials: S
authors.lastname: Arai
authors.forename: Kiyotaka Y
authors.initials: KY
authors.lastname: Hara
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Yamada
authors.forename: Chiaki
authors.initials: C
authors.lastname: Ogino
authors.forename: Hideki
authors.initials: H
authors.lastname: Fukuda
authors.forename: Akihiko
authors.initials: A
authors.lastname: Kondo
mesh_terms: D000681:Amylases / Q000235:genetics / Q000378:metabolism; D005978:Glutathione / Q000096:biosynthesis*; D053858:Metabolic Networks and Pathways / Q000235:genetics; D030781:Organisms, Genetically Modified; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D013213:Starch / Q000378:metabolism*
chemical_list: D011994:Recombinant Proteins; D029701:Saccharomyces cerevisiae Proteins; D013213:Starch; D000681:Amylases; D005978:Glutathione