title: Rapid and sensitive loop-mediated isothermal amplification test for Clostridium difficile detection challenges cytotoxin B cell test and culture as gold standard.
abstract: Compared to the composite gold standard cytotoxin B assay and toxigenic culture, the loop-mediated isothermal amplification (LAMP) test for Clostridium difficile had a sensitivity and specificity of 98%, positive predictive value of 92%, and negative predictive value of >99%. A one-hour turnaround time for the LAMP test provides rapid diagnosis and cost savings.
authors.affiliation: Department of Laboratory Medicine, Microbiology, Örebro University Hospital, SE-701 85 Örebro, Sweden. torbjorn.noren@orebroll.se
authors.forename: Torbjörn
authors.initials: T
authors.lastname: Norén
authors.forename: Ingegärd
authors.initials: I
authors.lastname: Alriksson
authors.forename: Josefin
authors.initials: J
authors.lastname: Andersson
authors.forename: Thomas
authors.initials: T
authors.lastname: Akerlund
authors.forename: Magnus
authors.initials: M
authors.lastname: Unemo
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D016360:Clostridioides difficile / Q000235:genetics / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification*; D004761:Enterocolitis, Pseudomembranous / Q000175:diagnosis* / Q000382:microbiology; D006801:Humans; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D013997:Time Factors
chemical_list: D004269:DNA, Bacterial