title: Enhancing aspergiolide A production from a shear-sensitive and easy-foaming marine-derived filamentous fungus Aspergillus glaucus by oxygen carrier addition and impeller combination in a bioreactor.
abstract: Production enhancement of a novel antitumor compound aspergiolide A from shear-sensitive and easy-foaming marine-derived fungus Aspergillus glaucus HB1-19 in a 5-l stirred bioreactor was investigated. Two types of impellers, i.e., six-flat-blade disc turbine impeller (DT) and three-sector-blade pitched blade turbine impeller (PB) were used in this work. In cultures with fermentation medium, the combination of upper PB and lower DT led to the maximum dry biomass (13.8 g/l) and aspergiolide A production (19.3 mg/l). However, two PBs brought the highest aspergiolide A yield coefficient (1.9 mg/g dry biomass) despite it produced the lowest dry biomass (5.3 g/l). By contrast, two DTs and the upper DT and lower PB showed insignificant results. Feeding 0.35% (v/v) n-dodecane in cultures with upper PB and lower DT further improved aspergiolide A production by 31.0%, i.e., 25.3 mg/l, which is also 322% higher than that in the ordinary cultures with two DTs.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.
authors.forename: Menghao
authors.initials: M
authors.lastname: Cai
authors.forename: Xiangshan
authors.initials: X
authors.lastname: Zhou
authors.forename: Jian
authors.initials: J
authors.lastname: Lu
authors.forename: Weimin
authors.initials: W
authors.lastname: Fan
authors.forename: Chuanpeng
authors.initials: C
authors.lastname: Niu
authors.forename: Jiushun
authors.initials: J
authors.lastname: Zhou
authors.forename: Xueqian
authors.initials: X
authors.lastname: Sun
authors.forename: Li
authors.initials: L
authors.lastname: Kang
authors.forename: Yuanxing
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000880:Anthraquinones / Q000378:metabolism*; D001230:Aspergillus / Q000378:metabolism*; D019149:Bioreactors / Q000382:microbiology*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005740:Gases / Q000737:chemistry; D009792:Oceans and Seas; D010100:Oxygen / Q000737:chemistry*; D012212:Rheology / Q000295:instrumentation*; D033081:Shear Strength
chemical_list: D000880:Anthraquinones; D005740:Gases; C542894:aspergiolide A; D010100:Oxygen