title: Microlens array recording of localized retinal responses.
abstract: We designed a rapid functional imager for the parallel recording of localized intrinsic optical signals (IOSs). This imager used a microlens array (MLA)-based illuminator to deliver visible stimulus light and near-infrared (NIR) recording light simultaneously. The parfocal configuration of the stimulus and recording light illumination enabled confocal recording of the stimulus-evoked IOSs. Because the MLA stimulation/recording spots were widely separated on the retina, and only the photoreceptors within the MLA stimulation/recording spots were stimulated, the potential IOS cross talk effect among neighboring retinal areas was minimized. Our experiments revealed robust IOS activities tightly correlated with localized retinal responses.
authors.affiliation: Department of Biomedical Engineering, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA.
authors.forename: Qiu-Xiang
authors.initials: QX
authors.lastname: Zhang
authors.forename: Jin-Yu
authors.initials: JY
authors.lastname: Wang
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
authors.forename: Xin-Cheng
authors.initials: XC
authors.lastname: Yao
mesh_terms: D000818:Animals; D004867:Equipment Design; D066298:In Vitro Techniques; D007909:Lenses; D018613:Microscopy, Confocal / Q000295:instrumentation; D008904:Miniaturization; D055620:Optical Phenomena; D010775:Photic Stimulation / Q000295:instrumentation*; D011894:Rana pipiens; D012160:Retina / Q000502:physiology*; D019265:Spectroscopy, Near-Infrared