title: T cells acquire cell surface determinants of APC via in vivo trogocytosis during viral infections.
abstract: Trogocytosis describes the transfer of surface determinants between immune cells and has been implicated in immune regulation. Most findings are based on in vitro studies since in vivo trogocytosis of immune cells is difficult to detect under physiological conditions. We used low frequencies of memory P14 T cells to demonstrate that T cells perform trogocytosis in vivo if in contact with APC pulsed with GP33-peptide or expressing the antigen endogenously. Furthermore, in vivo trogocytosis of T cells is demonstrated during infections with lymphocytic choriomeningitis virus and vaccinia virus. Trogocytosis-positive T cells revealed higher expression of activation marker and cytokines, showing a more activated phenotype compared to trogocytosis-negative T cells.
authors.affiliation: Institute for Medical Microbiology and Hygiene, Department of Immunology, University of Freiburg, Freiburg, Germany.
authors.forename: Kerstin
authors.initials: K
authors.lastname: Rosenits
authors.forename: Selina J
authors.initials: SJ
authors.lastname: Keppler
authors.forename: Smiljka
authors.initials: S
authors.lastname: Vucikuja
authors.forename: Peter
authors.initials: P
authors.lastname: Aichele
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D000954:Antigens, Surface / Q000378:metabolism; D000956:Antigens, Viral / Q000276:immunology; D001117:Arenaviridae Infections / Q000276:immunology*; D002478:Cells, Cultured; D006023:Glycoproteins / Q000276:immunology; D008213:Lymphocyte Activation; D008217:Lymphocytic choriomeningitis virus / Q000276:immunology* / Q000472:pathogenicity; D008561:Membrane Fusion / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D010446:Peptide Fragments / Q000276:immunology; D011948:Receptors, Antigen, T-Cell / Q000235:genetics; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism* / Q000473:pathology / Q000821:virology; D014764:Viral Proteins / Q000276:immunology
chemical_list: D000954:Antigens, Surface; D000956:Antigens, Viral; D006023:Glycoproteins; D010446:Peptide Fragments; D011948:Receptors, Antigen, T-Cell; D014764:Viral Proteins; C094244:glycoprotein peptide 33-41, Lymphocytic choriomeningitis virus