title: Use of inverse PCR for analysis of class 1 integrons carrying an unusual 3' conserved segment structure.
abstract: By using inverse PCR and DNA sequencing, 13 sul3-associated mutational integrons, 2 defective class 1 integrons, and 1 qnrB2-associated complex sul1-type class 1 integrons were identified in Salmonella enterica serovar Choleraesuis, Pseudomonas aeruginosa, and Enterobacter cloacae, respectively. In addition, conjugation and Southern hybridization demonstrated that unusual class 1 integrons were located on plasmids or integrated into chromosomal DNA. Thus, an inverse PCR assay can be a valuable tool for the analysis of unusual structures of the 3' conserved region of class 1 integrons.
authors.affiliation: Department of Medical Laboratory Science and Biotechnology, College of Health Sciences, Kaohsiung Medical University, Kaohsiung, Taiwan.
authors.forename: Mei-Feng
authors.initials: MF
authors.lastname: Lee
authors.forename: Chien-Fang
authors.initials: CF
authors.lastname: Peng
authors.forename: Hui-Jine
authors.initials: HJ
authors.lastname: Hsu
authors.forename: Han-Siong
authors.initials: HS
authors.lastname: Toh
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D015139:Blotting, Southern; D003227:Conjugation, Genetic / Q000235:genetics; D017124:Conserved Sequence; D024881:Drug Resistance, Bacterial / Q000235:genetics; D016972:Enterobacter cloacae / Q000187:drug effects / Q000235:genetics*; D006801:Humans; D032023:Integrons / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000235:genetics*; D019779:Salmonella enterica / Q000145:classification / Q000187:drug effects / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013449:Sulfonamides / Q000494:pharmacology
chemical_list: D001426:Bacterial Proteins; C489157:Sul3 protein, Salmonella enterica; D013449:Sulfonamides