title: Ribonuclease S redux.
abstract: The S-peptide and S-protein components of bovine pancreatic ribonuclease form a noncovalent complex with restored ribonucleolytic activity. Although this archetypal protein-fragment complementation system has been the object of historic work in protein chemistry, intrinsic limitations compromise its utility. Modern methods are shown to overcome those limitations and enable new applications.
authors.affiliation: Department of Biochemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706-1544, USA.
authors.forename: Rex W
authors.initials: RW
authors.lastname: Watkins
authors.forename: Ulrich
authors.initials: U
authors.lastname: Arnold
authors.forename: Ronald T
authors.initials: RT
authors.lastname: Raines
mesh_terms: D000818:Animals; D001412:Bacillus subtilis / Q000201:enzymology*; D002417:Cattle; D020372:Dexfenfluramine; D008958:Models, Molecular; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D011487:Protein Conformation; D012259:Ribonuclease, Pancreatic / Q000737:chemistry* / Q000378:metabolism*; D020860:Subtilisin / Q000378:metabolism*
chemical_list: D010446:Peptide Fragments; D020372:Dexfenfluramine; D012259:Ribonuclease, Pancreatic; D020860:Subtilisin