title: Term placenta shows methylation independent down regulation of Cyp19 gene in animals with retained fetal membranes.
abstract: Retention of fetal membranes (RFM) is the major post-partum disorder in dairy cattle. Cyp19 gene encodes the aromatase enzyme responsible for catalyzing the rate limiting step in estrogen biosynthesis, an important hormone for placental maturation and expulsion. The present study was aimed for comparative analysis of Cyp19 gene expression and its epigenetic regulation in placental cotyledons of animals with and without RFM. Significantly lower expression of Cyp19 gene was found in placental samples of RFM affected animals in comparison to normal animals. Methylation analysis of 5 CpG dinucleotides of placenta specific Cyp19 gene promoter I.1 and proximal promoter, PII showed hypo-methylation of both PI.1 and PII in term placenta of normal and diseased animals. In conclusion, a mechanism other than promoter methylation is responsible for decreased aromatase expression in placental cotyledons of animals suffering from RFM.
authors.affiliation: National Dairy Research Institute, Karnal-132001, Haryana, India.
authors.forename: Sandeep
authors.initials: S
authors.lastname: Ghai
authors.forename: Rachna
authors.initials: R
authors.lastname: Monga
authors.forename: T K
authors.initials: TK
authors.lastname: Mohanty
authors.forename: M S
authors.initials: MS
authors.lastname: Chauhan
authors.forename: Dheer
authors.initials: D
authors.lastname: Singh
mesh_terms: D000818:Animals; D001141:Aromatase / Q000235:genetics* / Q000378:metabolism; D002020:Buffaloes / Q000235:genetics* / Q000378:metabolism; D016022:Case-Control Studies; D019175:DNA Methylation; D015536:Down-Regulation; D044127:Epigenesis, Genetic; D005321:Extraembryonic Membranes / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D010920:Placenta / Q000378:metabolism*; D018457:Placenta, Retained / Q000235:genetics / Q000378:metabolism / Q000662:veterinary*; D011247:Pregnancy; D011248:Pregnancy Complications / Q000235:genetics / Q000378:metabolism / Q000662:veterinary*; D011401:Promoter Regions, Genetic; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary
chemical_list: D001141:Aromatase