title: Genetic screening in couples experiencing recurrent assisted procreation failure.
abstract: Infertility is a major health problem affecting about 10-20% of couples in the reproductive age group. Male factor is assumed to be responsible in about 50% cases of infertility. The origin of reduced testicular sperm function is unknown in about 50-70% of cases and for such couples assisted reproduction techniques (ART) are a boon. Male infertility is often due to poor semen quality and may be associated with genetic defects. ART has revolutionized management of infertility and intracytoplasmic sperm injection (ICSI) is the ART procedure of choice in 60-80% cases. Despite major technological advancements and professional expertise in ART, the success rate and carry-home live birth rate of ICSI is low (18-25%). This study was aimed to understand the genetic etiopathology of recurrent ART failure. For this, 110 couples with 3 or more failed ART cycles were recruited. A detailed history was taken and only idiopathic ART failure cases were enrolled for this study. They were subjected to cytogenetic and Yq microdeletion analysis. Genetic abnormalities were detected in 19 couples. Since a large number (18.2%) cases harboured genetic abnormalities, it is important for all couples opting for ART to undergo a thorough genetic analysis to prevent recurrent emotional, physical and financial stress.
authors.affiliation: Laboratory of Molecular Reproduction and Genetics, Department of Anatomy, All India Institute of Medical Sciences, New Delhi, 110029, India. rima_dada@rediffmail.com
authors.forename: Rima
authors.initials: R
authors.lastname: Dada
authors.forename: R
authors.initials: R
authors.lastname: Kumar
authors.forename: M B
authors.initials: MB
authors.lastname: Shamsi
authors.forename: M
authors.initials: M
authors.lastname: Tanwar
authors.forename: D
authors.initials: D
authors.lastname: Pathak
authors.forename: S
authors.initials: S
authors.lastname: Venkatesh
authors.forename: M
authors.initials: M
authors.lastname: Kumar
authors.forename: H
authors.initials: H
authors.lastname: Singh
authors.forename: K
authors.initials: K
authors.lastname: Singh
authors.forename: M
authors.initials: M
authors.lastname: Aron
authors.forename: R
authors.initials: R
authors.lastname: Kumar
authors.forename: G
authors.initials: G
authors.lastname: Singh
authors.forename: R K
authors.initials: RK
authors.lastname: Sharma
authors.forename: N P
authors.initials: NP
authors.lastname: Gupta
mesh_terms: D001483:Base Sequence; D002869:Chromosome Aberrations; D017931:DNA Primers; D005260:Female; D005820:Genetic Testing; D006801:Humans; D008297:Male; D027724:Reproductive Techniques, Assisted
chemical_list: D017931:DNA Primers