title: Role of protein kinase C and mitochondrial permeability transition pore in the neuroprotective effect of ceramide in ischemia-induced cell death.
abstract: In this study, we investigated the role of protein kinase C (PKC) and mitochondrial permeability transition pore (mPTP) on the effect of ceramide in an in vitro model of ischemia in SH-SY5Y neuroblastoma cells. In ischemic cell viability studies, a dual effect of ceramide was observed, depending on ceramide concentration. PKC isoforms are involved in the protective effect of low concentrations of ceramide. During ischemia, ceramide treatment leads to an increase in the formation of reactive oxygen species (ROS), which induces a controlled opening of mPTP. This fact prevents mitochondrial Ca(2+) overload, which is clearly protective.
authors.affiliation: Departamento de Bioquímica y Biología Molecular I, Facultad de Química, Universidad Complutense de Madrid, Madrid, Spain.
authors.forename: Alba
authors.initials: A
authors.lastname: Agudo-López
authors.forename: Begoña G
authors.initials: BG
authors.lastname: Miguel
authors.forename: Inmaculada
authors.initials: I
authors.lastname: Fernández
authors.forename: Ana M
authors.initials: AM
authors.lastname: Martínez
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D002118:Calcium / Q000378:metabolism; D015687:Cell Hypoxia; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002518:Ceramides / Q000494:pharmacology*; D005947:Glucose / Q000494:pharmacology; D006801:Humans; D018613:Microscopy, Confocal; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D033681:Mitochondrial Membrane Transport Proteins / Q000502:physiology*; D000083162:Mitochondrial Permeability Transition Pore; D009447:Neuroblastoma / Q000378:metabolism / Q000473:pathology; D011493:Protein Kinase C / Q000378:metabolism*; D051744:Protein Kinase C-epsilon / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D000099221:Protein Kinase C zeta
chemical_list: D002118:Calcium; D002518:Ceramides; D005947:Glucose; D033681:Mitochondrial Membrane Transport Proteins; D000083162:Mitochondrial Permeability Transition Pore; D011493:Protein Kinase C; D051744:Protein Kinase C-epsilon; D017382:Reactive Oxygen Species; D000099221:Protein Kinase C zeta