title: An integrated, low noise patch-clamp amplifier for biological nanopore applications.
abstract: We present an integrated, low noise patch-clamp amplifier for biological nanopore applications. Our amplifier consists of an integrator-differentiator architecture coupled with a novel opamp design in the CMOS 0.35 µm process. The post-layout full-chip simulation shows the input referred noise of the amplifier is 0.49 pA RMS over a 5 kHz bandwidth using a verified electrical model for the biological nanopore system. In our biological nanopore experiments studying protein-DNA interactions, we encounter capacitive transients with a nominal settling time of 5 ms. Our amplifier design reduces the settling time to 0.2 ms, without requiring any compensation circuitry.
authors.affiliation: Computer Engineering, University of California, Santa Cruz, 1156 High Street, CA 95064, USA.
authors.forename: Gang
authors.initials: G
authors.lastname: Wang
authors.forename: William B
authors.initials: WB
authors.lastname: Dunbar
mesh_terms: D000465:Algorithms; D000669:Amplifiers, Electronic; D001695:Biology / Q000379:methods; D001698:Biomedical Engineering / Q000379:methods; D003198:Computer Simulation; D004247:DNA / Q000737:chemistry; D004277:DNA, Single-Stranded / Q000235:genetics; D004594:Electrophysiology / Q000379:methods; D004867:Equipment Design; D029968:Escherichia coli Proteins / Q000737:chemistry; D006460:Hemolysin Proteins / Q000737:chemistry; D007700:Kinetics; D058608:Nanopores; D036103:Nanotechnology / Q000379:methods*; D018408:Patch-Clamp Techniques / Q000379:methods*; D012984:Software
chemical_list: D004277:DNA, Single-Stranded; D029968:Escherichia coli Proteins; D006460:Hemolysin Proteins; C036507:Hlya protein, E coli; D004247:DNA