title: Knockdown of TSP50 inhibits cell proliferation and induces apoptosis in P19 cells.
abstract: Earlier studies identified testes-specific protease 50 (TSP50), which encodes a threonine protease, and showed that it was abnormally reactivated in many breast cancer biopsies. Further, it was shown to be negatively regulated by the p53 gene. However, little is known about the biological function of TSP50. In this study, we applied RNA interference to knockdown TSP50 gene expression in P19 murine embryonal carcinoma stem cells and tested whether this modulated the cell phenotype. The results showed that downregulation of TSP50 expression not only reduced cell proliferation, colony formation, and migration but also induced cell apoptosis. Further investigation revealed that knockdown of TSP50 resulted in greater sensitivity to doxorubicin-induced apoptosis and that activation of caspase-3 was involved in this process.
authors.affiliation: National Engineering Laboratory for Druggable Gene and Protein Screening, Northeast Normal University, Changchun 130024, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Zhou
authors.forename: Yong-Li
authors.initials: YL
authors.lastname: Bao
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yin
authors.initials: Y
authors.lastname: Wu
authors.forename: Chun-Lei
authors.initials: CL
authors.lastname: Yu
authors.forename: Yan-Xin
authors.initials: YX
authors.lastname: Huang
authors.forename: Ying
authors.initials: Y
authors.lastname: Sun
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Zheng
authors.forename: Yu-Xin
authors.initials: YX
authors.lastname: Li
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D015536:Down-Regulation; D004317:Doxorubicin / Q000494:pharmacology; D055785:Gene Knockdown Techniques; D051379:Mice; D034622:RNA Interference; D012697:Serine Endopeptidases / Q000235:genetics / Q000502:physiology*; D016159:Tumor Suppressor Protein p53 / Q000502:physiology
chemical_list: D016159:Tumor Suppressor Protein p53; D004317:Doxorubicin; D012697:Serine Endopeptidases; C446379:testis-specific protease 50