title: Fluorimetric assay of interaction of protein with ferrofluids.
abstract: Magnetic iron oxide nanoparticles are inherently biocompatible and are amenable to post synthesis surface modification, making them excellent candidates for many important applications. If the above can be achieved in a single-step i.e., in situ synthesis and functionalization, the results are expected to be more dramatic for sensitive detection of biomolecules. For any application, it is necessary to confer a high level of binding specificity through surface chemistry, which can be introduced by using biological moieties that possess lock-and-key interactions, like those observed in antibody-antigen and enzyme-substrate recognition. In this paper, we have synthesized water based ferrofluids with serum albumin, the major protein component of blood. A series of other ferrofluids using different biocompatible polymers have also been studied with respect to their size determined by transmission electron microscopy, magnetic behavior with the aid of vibrating sample magnetometry and binding capability to bovine serum albumin by quenching of its native fluorescence. From our results, it can be inferred that binding has taken place between magnetic particles and biomolecules, the binding constants of which indirectly reveal the efficiency of the interaction.
authors.affiliation: National Metallurgical Laboratory, Jamshedpur 831007, India. dhritimallik@nmlindia.org
authors.forename: Dhriti
authors.initials: D
authors.lastname: Mallik
authors.forename: Aparna
authors.initials: A
authors.lastname: Mir
authors.forename: Soumya
authors.initials: S
authors.lastname: Bhattacharya
authors.forename: Suprabha
authors.initials: S
authors.lastname: Nayar
mesh_terms: D000818:Animals; D002417:Cattle; D005453:Fluorescence; D005470:Fluorometry / Q000379:methods*; D058085:Iron Compounds / Q000378:metabolism*; D008280:Magnetics; D046529:Microscopy, Electron, Transmission; D011485:Protein Binding; D012710:Serum Albumin, Bovine / Q000378:metabolism*; D017550:Spectroscopy, Fourier Transform Infrared; D014732:Vibration
chemical_list: D058085:Iron Compounds; D012710:Serum Albumin, Bovine