title: Metabolite analysis by supercritical fluid chromatography.
abstract: Owing to its favorable properties, such as low viscosity and high diffusivity, a supercritical fluid can be used as the mobile phase in chromatography. Supercritical fluid chromatography (SFC) can provide high-speed and high-resolution separation. Since supercritical carbon dioxide (SCCO(2)), which is generally used as the mobile phase in SFC, is automatically emitted at room temperature, SFC is most commonly used as a preparative method. However, SFC can also be used to perform high-precision biomolecular analysis, especially for hydrophobic metabolites, because of the low polarity of SCCO(2). The use of a mass spectrometer with SFC can widen the scope of application of SFC to bioanalysis. In this review, we summarize practical application of SFC as a tool for the analysis of metabolites in real biological samples.
authors.affiliation: Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Osaka, Japan.
authors.forename: Atsuki
authors.initials: A
authors.lastname: Matsubara
authors.forename: Eiichiro
authors.initials: E
authors.lastname: Fukusaki
authors.forename: Takeshi
authors.initials: T
authors.lastname: Bamba
mesh_terms: D002245:Carbon Dioxide / Q000737:chemistry; D002338:Carotenoids / Q000032:analysis; D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D005419:Flavonoids / Q000032:analysis; D006801:Humans; D008055:Lipids / Q000032:analysis; D013058:Mass Spectrometry / Q000379:methods*; D055432:Metabolomics / Q000295:instrumentation / Q000379:methods*; D010636:Phenols / Q000032:analysis; D059808:Polyphenols
chemical_list: D005419:Flavonoids; D008055:Lipids; D010636:Phenols; D059808:Polyphenols; D002245:Carbon Dioxide; D002338:Carotenoids