title: The same synaptic vesicles drive active and spontaneous release.
abstract: Synaptic vesicles release neurotransmitter both actively (on stimulation) and spontaneously (at rest). It has been assumed that identical vesicles use both modes of release; however, recent evidence has challenged this view. Using several assays (FM dye imaging, pHluorin imaging and antibody-labeling of synaptotagmin) in neuromuscular preparations from Drosophila, frog and mouse, as well as rat cultured neurons, we found that the same vesicles participate in active and spontaneous release.
authors.affiliation: STED Microscopy Group, European Neuroscience Institute, Deutsche Forschungsgemeinschaft Center for Molecular Physiology of the Brain/Excellence Cluster 171, Göttingen, Germany.
authors.forename: Benjamin G
authors.initials: BG
authors.lastname: Wilhelm
authors.forename: Teja W
authors.initials: TW
authors.lastname: Groemer
authors.forename: Silvio O
authors.initials: SO
authors.lastname: Rizzoli
mesh_terms: D000818:Animals; D001001:Anura; D002478:Cells, Cultured; D004331:Drosophila melanogaster; D004705:Endocytosis / Q000502:physiology*; D051379:Mice; D009469:Neuromuscular Junction / Q000166:cytology / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013045:Species Specificity; D009435:Synaptic Transmission / Q000502:physiology*; D013572:Synaptic Vesicles / Q000378:metabolism*