title: Resveratrol reduces oxidative stress and cell death and increases mitochondrial antioxidants and XIAP in PC6.3-cells.
abstract: Resveratrol, a polyphenol derived e.g. from red grapes, has been shown to mediate several positive biological actions such as protection of cells against oxidative stress. It can also influence cell signaling, but the mechanisms behind its antioxidant properties are largely unknown. Here we show that RSV reduces oxidative stress and enhances cell survival in PC6.3 cells depending on the concentration. In these cells, RSV increased the levels of antioxidants, SOD2 and TRX2, and of X chromosome-linked inhibitor of apoptosis protein. RSV also activated NFκB signaling as shown using luciferase reporter constructs. These findings show that RSV regulates oxidative stress and mitochondrial antioxidants in neuronal cells. This may contribute to cell protection in various brain disorders.
authors.affiliation: Minerva Institute for Medical Research, Biomedicum-2 Helsinki, Tukholmankatu 8, FIN-00290 Helsinki, Finland. minna.kairisalo@helsinki.fi
authors.forename: Minna
authors.initials: M
authors.lastname: Kairisalo
authors.forename: Alessandra
authors.initials: A
authors.lastname: Bonomo
authors.forename: Alise
authors.initials: A
authors.lastname: Hyrskyluoto
authors.forename: Giuseppa
authors.initials: G
authors.lastname: Mudò
authors.forename: Natale
authors.initials: N
authors.lastname: Belluardo
authors.forename: Laura
authors.initials: L
authors.lastname: Korhonen
authors.forename: Dan
authors.initials: D
authors.lastname: Lindholm
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D015153:Blotting, Western; D002460:Cell Line; D006801:Humans; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000096:biosynthesis; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D016133:Polymerase Chain Reaction; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000096:biosynthesis; D013879:Thioredoxins / Q000096:biosynthesis; D051636:X-Linked Inhibitor of Apoptosis Protein / Q000096:biosynthesis*; D000099156:Superoxide Dismutase 2
chemical_list: D000975:Antioxidants; D024101:Mitochondrial Proteins; D000077185:Resveratrol; D013267:Stilbenes; D013482:Superoxide Dismutase; D013879:Thioredoxins; D051636:X-Linked Inhibitor of Apoptosis Protein; D000099156:Superoxide Dismutase 2; C467002:TXN2 protein, human