title: Normalized one-dimensional NOE measurements in isotopically labeled macromolecules using two-way cross-polarization.
abstract: A novel one-dimensional NOE experiment is presented where a selected proton is excited by two-way heteronuclear cross- polarization between protons and nitrogen-15 or carbon-13. The utility of the method is demonstrated for a sample of 15N labeled human ubiquitin. Inter- and intra-residue NOEs are clearly observed in a very time-effective manner. The signal intensities can be easily normalized.
authors.affiliation: Section de Chimie, Université de Lausanne, BCH, CH-1015, Lausanne, Switzerland.
authors.forename: E
authors.initials: E
authors.lastname: Chiarparin
authors.forename: P
authors.initials: P
authors.lastname: Pelupessy
authors.forename: B
authors.initials: B
authors.lastname: Cutting
authors.forename: T R
authors.initials: TR
authors.lastname: Eykyn
authors.forename: G
authors.initials: G
authors.lastname: Bodenhausen