title: [Human intra-intronic minisatellite UPS29 associated with neurological diseases regulates reporter gene EGFP expression depending on cell type].
abstract: Earlier, it was established that polymorphism of minisatellite UPS29 located in one of introns of human gene CENTB5 (ACAP3) was associated with Parkinson's disease and epilepsy. The main aim of this work was to elucidate if that minisatellite could regulate reporter gene activity, and if such activity was tissue (cell)-specific. To this end there was used transient transfection of HeLa cells, mouse embryonal carcinoma line F9, and rat astrocytes cultures with plasmides which contained reporter gene EGFP under eukaryotic promoter ROSA26 and different allelles of minisatellite UPS29. It was found that UPS29 possessed enhancer-like activity in neuronal type cells.
authors.forename: L K
authors.initials: LK
authors.lastname: Sasina
authors.forename: N A
authors.initials: NA
authors.lastname: Slominskaia
authors.forename: I O
authors.initials: IO
authors.lastname: Suchkova
authors.forename: E V
authors.initials: EV
authors.lastname: Pitsik
authors.forename: K V
authors.initials: KV
authors.lastname: Solov'ev
authors.forename: N A
authors.initials: NA
authors.lastname: Grudinina
authors.forename: T A
authors.initials: TA
authors.lastname: Klinskaia
authors.forename: E L
authors.initials: EL
authors.lastname: Patkin
mesh_terms: D000483:Alleles; D000818:Animals; D002478:Cells, Cultured; D004827:Epilepsy / Q000235:genetics*; D066246:ErbB Receptors / Q000235:genetics*; D020690:GTPase-Activating Proteins / Q000235:genetics*; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D006367:HeLa Cells; D006801:Humans; D007438:Introns / Q000235:genetics; D026901:Membrane Transport Proteins / Q000235:genetics*; D051379:Mice; D018598:Minisatellite Repeats / Q000235:genetics / Q000502:physiology*; D009928:Organ Specificity; D010300:Parkinson Disease / Q000235:genetics*; D051381:Rats
chemical_list: C542459:ACAP3 protein, human; D020690:GTPase-Activating Proteins; D026901:Membrane Transport Proteins; C512478:EGFR protein, human; D066246:ErbB Receptors