title: Paired immunoglobulin-like receptor B knockout does not enhance axonal regeneration or locomotor recovery after spinal cord injury.
abstract: Myelin components that inhibit axonal regeneration are believed to contribute significantly to the lack of axonal regeneration noted in the adult central nervous system. Three proteins found in myelin, Nogo, myelin-associated glycoprotein, and oligodendrocyte-myelin glycoprotein, inhibit neurite outgrowth in vitro. All of these proteins interact with the same receptors, namely, the Nogo receptor (NgR) and paired immunoglobulin-like receptor B (PIR-B). As per previous reports, corticospinal tract (CST) regeneration is not enhanced in NgR-knock-out mice after spinal cord injury. Therefore, we assessed CST regeneration in PIR-B-knock-out mice. We found that hindlimb motor function, as assessed using the Basso mouse scale, footprint test, inclined plane test, and beam walking test, did not differ between the PIR-B-knock-out and wild-type mice after dorsal hemisection of the spinal cord. Further, tracing of the CST fibers after injury did not reveal enhanced axonal regeneration or sprouting in the CST of the PIR-B-knock-out mice. Systemic administration of NEP1-40, a NgR antagonist, to PIR-B knock-out mice did not enhance the regenerative response. These results indicate that PIR-B knock-out is not sufficient to induce extensive axonal regeneration after spinal cord injury.
authors.affiliation: Department of Molecular Neuroscience, Graduate School of Medicine, Osaka University, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Yuka
authors.initials: Y
authors.lastname: Nakamura
authors.forename: Yuki
authors.initials: Y
authors.lastname: Fujita
authors.forename: Masaki
authors.initials: M
authors.lastname: Ueno
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Takai
authors.forename: Toshihide
authors.initials: T
authors.lastname: Yamashita
mesh_terms: D000818:Animals; D055785:Gene Knockdown Techniques; D006801:Humans; D051379:Mice; D018345:Mice, Knockout; D009043:Motor Activity; D009185:Myelin Proteins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D009186:Myelin Sheath / Q000235:genetics / Q000378:metabolism; D016501:Neurites / Q000378:metabolism* / Q000473:pathology; D000070798:Nogo Proteins; D010446:Peptide Fragments / Q000494:pharmacology; D011971:Receptors, Immunologic / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D020127:Recovery of Function; D012038:Regeneration; D013119:Spinal Cord Injuries / Q000378:metabolism* / Q000473:pathology
chemical_list: D009185:Myelin Proteins; C509624:NEP1-40 protein, human; D000070798:Nogo Proteins; D010446:Peptide Fragments; C105949:Pirb protein, mouse; C000603199:RTN4 protein, human; D011971:Receptors, Immunologic; C000603201:Rtn4 protein, mouse