title: Altered antisense-to-sense transcript ratios in breast cancer.
abstract: Transcriptome profiling studies suggest that a large fraction of the genome is transcribed and many transcripts function independent of their protein coding potential. The relevance of noncoding RNAs (ncRNAs) in normal physiological processes and in tumorigenesis is increasingly recognized. Here, we describe consistent and significant differences in the distribution of sense and antisense transcripts between normal and neoplastic breast tissues. Many of the differentially expressed antisense transcripts likely represent long ncRNAs. A subset of genes that mainly generate antisense transcripts in normal but not cancer cells is involved in essential metabolic processes. These findings suggest fundamental differences in global RNA regulation between normal and cancer cells that might play a role in tumorigenesis.
authors.affiliation: Department of Medical Oncology, and Belfer Institute for Applied Cancer Science, Dana-Farber Cancer Institute, Boston, MA 02115, USA.
authors.forename: Reo
authors.initials: R
authors.lastname: Maruyama
authors.forename: Michail
authors.initials: M
authors.lastname: Shipitsin
authors.forename: Sibgat
authors.initials: S
authors.lastname: Choudhury
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Wu
authors.forename: Alexei
authors.initials: A
authors.lastname: Protopopov
authors.forename: Jun
authors.initials: J
authors.lastname: Yao
authors.forename: Pang-Kuo
authors.initials: PK
authors.lastname: Lo
authors.forename: Marina
authors.initials: M
authors.lastname: Bessarabova
authors.forename: Alex
authors.initials: A
authors.lastname: Ishkin
authors.forename: Yuri
authors.initials: Y
authors.lastname: Nikolsky
authors.forename: X Shirley
authors.initials: XS
authors.lastname: Liu
authors.forename: Saraswati
authors.initials: S
authors.lastname: Sukumar
authors.forename: Kornelia
authors.initials: K
authors.lastname: Polyak
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics*; D030541:Databases, Genetic; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D052138:Genes, Neoplasm / Q000235:genetics; D006801:Humans; D016372:RNA, Antisense / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012334:RNA, Neoplasm / Q000235:genetics* / Q000378:metabolism; D015203:Reproducibility of Results; D059467:Transcriptome / Q000235:genetics
chemical_list: D016372:RNA, Antisense; D012333:RNA, Messenger; D012334:RNA, Neoplasm