title: A novel genetically-obese rat model with elevated 11 beta-hydroxysteroid dehydrogenase type 1 activity in subcutaneous adipose tissue.
abstract: 11 β-hydroxysteroid dehydrogenase type 1 (11 β-HSD1) catalyzes the conversion of inactive glucocorticoids to active glucocorticoids and plays an important role in the development of obesity and metabolic syndrome. 11 β-HSD1 activity is lower in liver and higher in omental adipose tissue of obese rodent models like obese zucker rats, Ob/Ob and db/db mice. Here, we report the 11 β-HSD1 activity in liver and adipose tissue of lean and obese rats of WNIN/Ob strain, a new genetic rat model of obesity. 11 β-HSD1 activity in liver, omental and subcutaneous adipose tissues of 3 month-old male WNIN/Ob lean and obese rats was assayed. As observed in other rodent models, 11 β-HSD1 activity was lower in liver and higher in omental adipose tissue. In contrast to other rodent obese models, WNIN/Ob obese rats had elevated 11 β-HSD1 activity in subcutaneous adipose tissue, which is in line with the observation in human obesity. Here, we conclude that dysregulation of 11 β-HSD1 in WNIN/Ob obese rat model is identical to human obesity, which makes it an excellent model for studying the effect of 11 β-HSD1 inhibitors in ameliorating obesity and metabolic syndrome.
authors.affiliation: Department of Biochemistry, National Institute of Nutrition, Jamai Osmania PO, Hyderabad-500 604, Andhra Pradesh, India. vaj_lipidresearch@yahoo.com.
authors.forename: Sakamuri S S Vara
authors.initials: SS
authors.lastname: Prasad
authors.forename: Anamthathmakula
authors.initials: A
authors.lastname: Prashanth
authors.forename: Chodavarapu Pavan
authors.initials: CP
authors.lastname: Kumar
authors.forename: Sirisha J
authors.initials: SJ
authors.lastname: Reddy
authors.forename: Nappan V
authors.initials: NV
authors.lastname: Giridharan
authors.forename: Ayyalasomayajula
authors.initials: A
authors.lastname: Vajreswari
mesh_terms: D043205:11-beta-Hydroxysteroid Dehydrogenase Type 1 / Q000378:metabolism*; D050154:Adiposity / Q000502:physiology; D000818:Animals; D007333:Insulin Resistance / Q000502:physiology / Q000528:radiation effects; D008297:Male; D051379:Mice; D008820:Mice, Obese; D009765:Obesity / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D051381:Rats; D050151:Subcutaneous Fat / Q000201:enzymology* / Q000378:metabolism
chemical_list: D043205:11-beta-Hydroxysteroid Dehydrogenase Type 1