title: Temporal diabetes and diuresis-induced alteration of nerves and vasculature of the urinary bladder in the rat.
abstract: OBJECTIVE
  • To characterize the temporal changes of the nerves and vasculature of the bladder in diabetic rats.
  
  
  METHODS
  • A total of 36 Sprague-Dawley rats were divided into three groups: streptozotocin-induced diabetics, 5% sucrose-induced diuretics and age-matched controls. • The characteristics of the nerves and vasculature in the equatorial cross-sectional areas of the bladder were examined by immunofluorescence staining of their specific markers, neurofilament 200 (NF200) and CD31, at 1, 9 or 20 weeks after induction. • The distributions of the nerves and blood vessels were observed and the densities were quantified.
  
  
  RESULTS
  • Diabetes caused a significant reduction in body weight. Bladder weight increased in diabetic and diuretic rats, but not in controls. • The total cross-sectional wall area and detrusor muscle area at the equatorial midline were greater in bladders of diabetic and diuretic rats than in controls. • Neurofilament 200-immunoreactive (NF200-IR) nerves were mainly distributed in the detrusor muscle. CD31-immunoreactive blood vessels were mainly distributed in the mucosa/submucosa. • There were no significant differences in the NF200-IR nerve terminal area among control, diabetic and diuretic groups. However nerve density was decreased at 9 and 20 weeks in the muscle, and at 20 weeks in the mucosa/submucosa in diabetic and diuretic animals. • Blood vessel density decreased in the diabetic and diuretic groups at 20 weeks in the muscle.
  
  
  CONCLUSIONS
  • Diabetes induced time-dependent changes in the density of the nerves and vasculature in the bladder tissues. • Diabetes-related polyuria plays an important role in these changes.
authors.affiliation: Department of Urology, Case Western Reserve University, Cleveland, OH 44106, USA. guiming.liu@case.edu
authors.forename: Guiming
authors.initials: G
authors.lastname: Liu
authors.forename: Mei
authors.initials: M
authors.lastname: Li
authors.forename: Amit
authors.initials: A
authors.lastname: Vasanji
authors.forename: Firouz
authors.initials: F
authors.lastname: Daneshgari
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000503:physiopathology*; D004231:Diuresis; D004232:Diuretics; D005455:Fluorescent Antibody Technique; D008297:Male; D009130:Muscle, Smooth / Q000098:blood supply* / Q000294:innervation* / Q000503:physiopathology; D016900:Neurofilament Proteins / Q000378:metabolism; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism; D011141:Polyuria / Q000503:physiopathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013395:Sucrose; D001743:Urinary Bladder / Q000098:blood supply* / Q000294:innervation* / Q000503:physiopathology
chemical_list: D015415:Biomarkers; D004232:Diuretics; D016900:Neurofilament Proteins; D019408:Platelet Endothelial Cell Adhesion Molecule-1; C051774:neurofilament protein H; D013395:Sucrose