title: Cd(II) and As(III) bioaccumulation by recombinant Escherichia coli expressing oligomeric human metallothioneins.
abstract: Metallothioneins (MTs) are a family of metal binding proteins. Recombinant Escherichia coli expressing the human MT (hMT-1A) gene was constructed for bioaccumulation of heavy metals. In order to increase protein stability, the glutathione S-transferase (GST) gene was fused with the hMT-1A gene and coexpressed. In order to increase MT expression efficiency and metal binding capacity, two, three or four hMT-1A genes were integrated in series and overexpressed in E. coli. The recombinant E. coli expressing the GST fused trimeric hMT-1A protein exhibited the highest Cd(II) and As(III) bioaccumulation ability, 6.36 mg Cd/g dry cells and 7.59 mg As/g dry cells, respectively.
authors.affiliation: State Key Lab of Microbial Technology, Shandong University, Shan Da Nan Lu Road 27#, Jinan 250100, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Ma
authors.forename: Jianqun
authors.initials: J
authors.lastname: Lin
authors.forename: Chengjia
authors.initials: C
authors.lastname: Zhang
authors.forename: Yilin
authors.initials: Y
authors.lastname: Ren
authors.forename: Jianqiang
authors.initials: J
authors.lastname: Lin
mesh_terms: D001151:Arsenic / Q000378:metabolism*; D001483:Base Sequence; D001704:Biopolymers / Q000235:genetics / Q000378:metabolism*; D002104:Cadmium / Q000378:metabolism*; D017931:DNA Primers; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008668:Metallothionein / Q000235:genetics / Q000378:metabolism*; D011995:Recombination, Genetic
chemical_list: D001704:Biopolymers; D017931:DNA Primers; D002104:Cadmium; D008668:Metallothionein; D001151:Arsenic