title: [Effects of oxymatrine injection combined with low-dose paclitaxel on mRNA and protein expressions of vascular endothelial growth factor and CXC chemokine receptor 4 in human gastric carcinoma SGC-7901 cells].
abstract: OBJECTIVE
  To investigate the effects of oxymatrine injection (OI) combined with low-dose paclitaxel on expressions of mRNAs and proteins of vascular endothelial growth factor (VEGF) and CXC chemokine receptor 4 (CXCR4) in human gastric carcinoma SGC-7901 cells.
  
  
  METHODS
  Methyl thiazolyl tetrazolium assay was used to examine the effects of OI combined with low-dose paclitaxel on proliferation of SGC-7901 cells. Real-time reverse transcription-polymerase chain reaction, immunofluorescence and enzyme-linked immunosorbent assay were employed to measure the expressions of VEGF and CXCR4 mRNAs and proteins in gastric carcinoma SGC-7901 cells respectively.
  
  
  RESULTS
  Except that 20 μg/mL paclitaxel had no influence on expression of VEGF mRNA in SGC-7901 cells (P>0.05), 40 μg/mL OI or low-dose paclitaxel (20 μg/mL) inhibited the proliferation of SGC-7901 cells and reduced the expressions of VEGF and CXCR4 mRNAs and proteins in SGC-7901 cells (P<0.01). The expressions of VEGF and CXCR4 mRNAs and proteins in the OI plus low-dose paclitaxel group were markedly lower than those in the low-dose paclitaxel group (P<0.01).
  
  
  CONCLUSIONS
  OI combined with low-dose paclitaxel can inhibit VEGF and CXCR4 of gastric carcinoma SGC-7901 cells markedly, which may be one of its mechanisms of anti-angiogenic ability.
authors.affiliation: Department of Gastroenterology, Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai 200233, China.
authors.forename: Yu-ren
authors.initials: YR
authors.lastname: Zhang
authors.forename: Jin-shui
authors.initials: JS
authors.lastname: Zhu
authors.forename: Xiao-yin
authors.initials: XY
authors.lastname: Wang
authors.forename: Zhi-peng
authors.initials: ZP
authors.lastname: Xu
authors.forename: Zhou
authors.initials: Z
authors.lastname: Zhou
authors.forename: Long
authors.initials: L
authors.lastname: Wang
authors.forename: Qun
authors.initials: Q
authors.lastname: Sun
authors.forename: Li
authors.initials: L
authors.lastname: Zhu
mesh_terms: D000470:Alkaloids / Q000008:administration & dosage / Q000494:pharmacology*; D045744:Cell Line, Tumor; D006801:Humans; D017239:Paclitaxel / Q000008:administration & dosage / Q000494:pharmacology*; D011807:Quinolizines / Q000008:administration & dosage / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics; D019718:Receptors, CXCR4 / Q000378:metabolism*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*; D000093842:Matrines
chemical_list: D000470:Alkaloids; D017239:Paclitaxel; D011807:Quinolizines; D012333:RNA, Messenger; D019718:Receptors, CXCR4; D042461:Vascular Endothelial Growth Factor A; C527276:CXCR4 protein, human; C467484:VEGFA protein, human; C037573:oxymatrine; D000093842:Matrines