title: DNA photo-oxidative damage hazard in transfection complexes.
abstract: Complexes of DNA with various cationic vectors have been largely used for nonviral transfection, and yet the photochemical stability of DNA in such complexes has never been considered. We studied, for the first time, the influence of DNA complexation by a cationic lipid and polymers on the amount of damage induced by benzophenone photosensitization. The localization of benzophenone inside the hydrophobic domains formed by a cationic lipid, DOTAP (N-[1-(2,3-dioleoyloxy)propyl]-N,N,N-trimethylammonium chloride), and close to DNA, locally increases the photoinduced cleavage by the reactive oxygen species generated. The same effect was found in the case of DNA complexation with an amphiphilic polymer (polynorbornenemethyleneammonium chloride). However, a decrease in DNA damage was observed in the case of complexation with a hydrophilic polymer (polyethylenimine). The DNA protection in this case was because of the absence of benzophenone hydrophobic incorporation into the complex, and to DNA compaction which decreased the probability of radical attack. These results underline the importance of the chemical structure of the nonviral transfection vector in limiting the risks of photo-oxidative damage of the complexed DNA.
authors.affiliation: Laboratoire des I.M.R.C.P. UMR 5623 CNRS, Université Paul Sabatier, Toulouse, France. rudyuk@ukr.net
authors.forename: Sergii
authors.initials: S
authors.lastname: Rudiuk
authors.forename: Sophie
authors.initials: S
authors.lastname: Franceschi-Messant
authors.forename: Nadia
authors.initials: N
authors.lastname: Chouini-Lalanne
authors.forename: Emile
authors.initials: E
authors.lastname: Perez
authors.forename: Isabelle
authors.initials: I
authors.lastname: Rico-Lattes
mesh_terms: D004249:DNA Damage; D004587:Electrophoresis, Agar Gel; D010084:Oxidation-Reduction; D010777:Photochemistry; D014162:Transfection