title: Matrix-assisted laser desorption ionization-time of flight mass spectrometry as an alternative to 16S rRNA gene sequencing for identification of difficult-to-identify bacterial strains.
abstract: Conventional methods are sometimes insufficient to identify human bacterial pathogens, and alternative techniques, often molecular, are required. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) identified with a valid score 45.9% of 410 clinical isolates from 207 different difficult-to-identify species having required 16S rRNA gene sequencing. MALDI-TOF MS might represent an alternative to 16S rRNA gene sequencing.
authors.affiliation: Institute of Microbiology, University of Lausanne and University Hospital Center, Rue du Bugnon 46, 1011 Lausanne, Switzerland.
authors.forename: A
authors.initials: A
authors.lastname: Bizzini
authors.forename: K
authors.initials: K
authors.lastname: Jaton
authors.forename: D
authors.initials: D
authors.lastname: Romo
authors.forename: J
authors.initials: J
authors.lastname: Bille
authors.forename: G
authors.initials: G
authors.lastname: Prod'hom
authors.forename: G
authors.initials: G
authors.lastname: Greub
mesh_terms: D001419:Bacteria / Q000737:chemistry* / Q000145:classification* / Q000302:isolation & purification; D001424:Bacterial Infections / Q000175:diagnosis* / Q000382:microbiology*; D001431:Bacteriological Techniques / Q000379:methods*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D006801:Humans; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S