title: Hydroxyapatite coatings deposited by liquid precursor plasma spraying: controlled dense and porous microstructures and osteoblastic cell responses.
abstract: Hydroxyapatite coatings were deposited on Ti-6Al-4V substrates by a novel plasma spraying process, the liquid precursor plasma spraying (LPPS) process. X-ray diffraction results showed that the coatings obtained by the LPPS process were mainly composed of hydroxyapatite. The LPPS process also showed excellent control on the coating microstructure, and both nearly fully dense and highly porous hydroxyapatite coatings were obtained by simply adjusting the solid content of the hydroxyapatite liquid precursor. Scanning electron microscope observations indicated that the porous hydroxyapatite coatings had pore size in the range of 10-200 µm and an average porosity of 48.26 ± 0.10%. The osteoblastic cell responses to the dense and porous hydroxyapatite coatings were evaluated with human osteoblastic cell MG-63, in respect of the cell morphology, proliferation and differentiation, with the hydroxyapatite coatings deposited by the atmospheric plasma spraying (APS) process as control. The cell experiment results indicated that the heat-treated LPPS coatings with a porous structure showed the best cell proliferation and differentiation among all the hydroxyapatite coatings. Our results suggest that the LPPS process is a promising plasma spraying technique for fabricating hydroxyapatite coatings with a controllable microstructure, which has great potential in bone repair and replacement applications.
authors.affiliation: National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, People's Republic of China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Huang
authors.forename: Lei
authors.initials: L
authors.lastname: Song
authors.forename: Xiaoguang
authors.initials: X
authors.lastname: Liu
authors.forename: Yanfeng
authors.initials: Y
authors.lastname: Xiao
authors.forename: Yao
authors.initials: Y
authors.lastname: Wu
authors.forename: Jiyong
authors.initials: J
authors.lastname: Chen
authors.forename: Fang
authors.initials: F
authors.lastname: Wu
authors.forename: Zhongwei
authors.initials: Z
authors.lastname: Gu
mesh_terms: D002454:Cell Differentiation; D002460:Cell Line; D049109:Cell Proliferation; D002621:Chemistry / Q000379:methods*; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D017886:Durapatite / Q000737:chemistry*; D006801:Humans; D010006:Osteoblasts / Q000737:chemistry / Q000166:cytology*; D016062:Porosity
chemical_list: D020099:Coated Materials, Biocompatible; D017886:Durapatite