title: [Influence of Japanese enciphalitis virus capsid protein on the self-replicate ability of JEV replicon vectors].
abstract: To optimize a self-replicate Japanese enciphalitis virus (JEV) replicon, and to make it as an efficient vector to express the heterologous protein, we constructed three JEV replicons by PCR-based shortening the length of capsid genes. The vectors remained full or part of C gene, based on the JEV replicon pCTCJEV. Lac Z was selected as the reporter gene to verify the self-replicate ability of these DNA-based replicons. While transfected into the cell lines CME-4, which continuously expressing the JEV structure proteins C-prM-E, the JEV replicons pCMW-2M-1LACZ, pCMW-2M-3LACZ, which remained the first 23aa and 68aa of C protein, can express the reporter protein as the same level as pCMW-2M-LACZ with the full-length C protein. These results illustrated that the JEV replicon vector with 69-nt of the C gene can retain the self-replicate ability, and provide valuable tools to construct a possible vector for a long-lasting JEV RNA virus expression system.
authors.affiliation: Laboratory for Protein Engineering, Institute ofBiotechnology, Academy of Military Medical Sciences, Beijing 100071, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Huang
authors.forename: Shan
authors.initials: S
authors.lastname: Liu
authors.forename: Peng
authors.initials: P
authors.lastname: Yang
authors.forename: Chao
authors.initials: C
authors.lastname: Wang
authors.forename: Yun
authors.initials: Y
authors.lastname: Du
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Sun
authors.forename: Weiyuan
authors.initials: W
authors.lastname: Yu
mesh_terms: D036022:Capsid Proteins / Q000235:genetics / Q000502:physiology*; D002460:Cell Line; D004664:Encephalitis Virus, Japanese / Q000235:genetics* / Q000502:physiology; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D012093:Replicon / Q000235:genetics* / Q000502:physiology; D014162:Transfection; D014779:Virus Replication / Q000235:genetics / Q000502:physiology*
chemical_list: D036022:Capsid Proteins