title: Multi-featured macroporous agarose-alginate cryogel: synthesis and characterization for bioengineering applications.
abstract: In this study agarose-alginate scaffolds are synthesized using cryogelation technology in different formats like monolith, sheet, discs, and beads, and show amiable mechanical strength like soft tissue properties and high interconnected macroporous degradable architecture. In cell-material interactions, fibroblast (NIH-3T3) cells showed good adherence and proliferation on these scaffolds presenting its potential application in soft tissue engineering. The application of cryogel beads and monoliths was also examined by the efficient immobilization of bacterial cells (BL21) on these matrices revealing their use for recovery of product from continuous fermentation systems without cell leakage. These scaffolds also showed potential as a filter for repeated recovery of heavy metal binding, such as copper and nickel from the waste water. The cryogels prepared herein do have a number of unique features that make them an important class of soft materials for developing multi-featured scaffolds as a novel carrier for bioengineering applications.
authors.affiliation: Department of Biological Sciences and Bioengineering, Indian Institute of Technology Kanpur, 208016 Kanpur, India.
authors.forename: Anuj
authors.initials: A
authors.lastname: Tripathi
authors.forename: Ashok
authors.initials: A
authors.lastname: Kumar
mesh_terms: D000464:Alginates / Q000737:chemistry*; D000818:Animals; D001422:Bacterial Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D019245:Compressive Strength; D019327:Copper Sulfate / Q000737:chemistry; D059985:Cryogels; D004355:Drug Stability; D004926:Escherichia coli / Q000187:drug effects / Q000502:physiology; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D020100:Hydrogels / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D008422:Materials Testing; D051379:Mice; D041681:NIH 3T3 Cells; D009532:Nickel / Q000737:chemistry; D010539:Permeability; D016062:Porosity; D012685:Sepharose / Q000737:chemistry*; D013046:Specific Gravity; D013314:Stress, Mechanical; D055114:X-Ray Microtomography
chemical_list: D000464:Alginates; D059985:Cryogels; D006603:Hexuronic Acids; D020100:Hydrogels; C029938:nickel sulfate; D009532:Nickel; D020723:Glucuronic Acid; D012685:Sepharose; D019327:Copper Sulfate