title: A reverse-transcription, loop-mediated isothermal amplification assay for detection of bovine ephemeral fever virus in the blood of infected cattle.
abstract: A novel reverse-transcription, loop-mediated isothermal amplification (RT-LAMP) assay for the detection of bovine ephemeral fever virus (BEFV) was developed and evaluated in this study. The RT-LAMP assay exhibited higher sensitivity when compared with conventional reverse-transcription polymerase chain reaction (RT-PCR) and virus isolation methods. The specificity of the assay was determined by digestion of the RT-LAMP products with restriction enzyme and detection of BEFV serogroup rabies virus (RV). Using RT-LAMP, RT-PCR and virus isolation methods, 36 blood samples were tested and the results indicated that RT-LAMP could detect early infection with BEFV. The RT-LAMP method is useful for the diagnosis of BEFV infection in blood samples.
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Yanchangbao, Lanzhou, China.
authors.forename: Fuying
authors.initials: F
authors.lastname: Zheng
authors.forename: Guozhen
authors.initials: G
authors.lastname: Lin
authors.forename: Jizhang
authors.initials: J
authors.lastname: Zhou
authors.forename: Guanghua
authors.initials: G
authors.lastname: Wang
authors.forename: Xiaoan
authors.initials: X
authors.lastname: Cao
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Gong
authors.forename: Changqing
authors.initials: C
authors.lastname: Qiu
mesh_terms: D000818:Animals; D002417:Cattle; D004810:Ephemeral Fever / Q000175:diagnosis* / Q000821:virology; D018115:Ephemeral Fever Virus, Bovine / Q000235:genetics / Q000302:isolation & purification*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012367:RNA, Viral / Q000097:blood* / Q000235:genetics; D048348:Reverse Transcription; D012680:Sensitivity and Specificity; D014773:Virology / Q000379:methods*
chemical_list: D012367:RNA, Viral