title: [The cytoskeleton and immigration of laryngeal squamous carcinoma cell affected by Fascin-1 expression].
abstract: This study sought to detect the effect of Fascin-1 expression on the cytoskeleton and immigration of laryngeal squamous carcinoma cell. In the experiment, Fascin-1 expression in Hep-2 cells was inhibited by small interfering RNA. The cytoskeleton of Hep-2 cells was observed with the use of laser scanning confocal fluorescence microscope. Millicell insert was applied to detect the immigration of Hep-2 cells in vitro. The results showed that the integrity of cytoskeleton in Hep-2 cells was broken with the down-regulation of Fascin-1 expression and the immigration ability was decreased significantly (P < 0.05). The inhibiting ratio of cell immigration was 44.6 +/- 6.3%. In conclusion, inhibition of Fascin-1 expression in Hep-2 cells could break the integrity of cytoskeleton and decrease the ability of cellular immigration.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, West China Hospital, Sichuan University, Chengdu 610041, China. zoujian926@163.com
authors.forename: Jian
authors.initials: J
authors.lastname: Zon
authors.forename: Fei
authors.initials: F
authors.lastname: Chen
authors.forename: Jiang
authors.initials: J
authors.lastname: Wu
authors.forename: Wen
authors.initials: W
authors.lastname: Li
authors.forename: Lin
authors.initials: L
authors.lastname: Lou
authors.forename: Shixi
authors.initials: S
authors.lastname: Liu
authors.forename: Hui
authors.initials: H
authors.lastname: Yang
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000648:ultrastructure; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D002465:Cell Movement; D003599:Cytoskeleton / Q000378:metabolism / Q000648:ultrastructure; D015536:Down-Regulation; D006801:Humans; D007822:Laryngeal Neoplasms / Q000378:metabolism* / Q000648:ultrastructure; D008840:Microfilament Proteins / Q000235:genetics / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000235:genetics
chemical_list: D002352:Carrier Proteins; C502658:FSCN1 protein, human; D008840:Microfilament Proteins; D034741:RNA, Small Interfering