title: Detection of myocardial ischaemia using surface microdialysis on the beating heart.
abstract: Microdialysis (MD) can be used to study metabolism of the beating heart. We investigated whether microdialysis results obtained from epicardial (surface) sampling reflect acute changes in the same way as myocardial sampling from within the substance of the ventricular wall. In anaesthetized open-thorax pigs a coronary snare was placed. One microdialysis probe was placed with the sampling membrane intramyocardially (myocardial), and a second probe was placed with the sampling membrane epicardially (surface), both in the area which was made ischaemic. Ten minutes collection intervals were used for microdialysis samples. Samples from 19 pigs were analysed for lactate, glucose, pyruvate and glycerol during equilibration, baseline, ischaemia and reperfusion periods. For both probes (surface and myocardial), a total of 475 paired simultaneous samples were analysed. Results from analyses showed no differences in relative changes for glucose, lactate and glycerol during baseline, ischaemia and reperfusion. Surface microdialysis sampling is a new application of the microdialysis technique that shows promise and should be further studied.
authors.affiliation: Department of Surgical and Perioperative Sciences, Anaesthesiology and Intensive Care Medicine, University Hospital of Umeå, Umeå, Sweden. pernilla.abrahamsson@anestesi.umu.se
authors.forename: Pernilla
authors.initials: P
authors.lastname: Abrahamsson
authors.forename: Anna-Maja
authors.initials: AM
authors.lastname: Aberg
authors.forename: Göran
authors.initials: G
authors.lastname: Johansson
authors.forename: Ola
authors.initials: O
authors.lastname: Winsö
authors.forename: Anders
authors.initials: A
authors.lastname: Waldenström
authors.forename: Michael
authors.initials: M
authors.lastname: Haney
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D004195:Disease Models, Animal; D004734:Energy Metabolism; D005260:Female; D005947:Glucose / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D019344:Lactic Acid / Q000378:metabolism; D017551:Microdialysis; D017202:Myocardial Ischemia / Q000175:diagnosis* / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism*; D010496:Pericardium / Q000378:metabolism*; D011237:Predictive Value of Tests; D019289:Pyruvic Acid / Q000378:metabolism; D013552:Swine; D013997:Time Factors
chemical_list: D015415:Biomarkers; D019344:Lactic Acid; D019289:Pyruvic Acid; D005947:Glucose; D005990:Glycerol