title: Mechanisms underlying the diabetes-induced hyporeactivity of the rabbit carotid artery to atrial natriuretic peptide.
abstract: Atrial natriuretic peptide (ANP) plays an important role in the pathophysiology of the vascular complications in diabetes. The working hypothesis was that diabetes might modify the vascular actions of ANP in isolated rabbit carotid arteries and the mechanisms involved in these actions. ANP (10(-12)-10(-7)M) induced a relaxation of precontracted carotid arteries, which was lower in diabetic than in control rabbits. In arteries from both groups of animals, endothelium removal increased the ANP-induced relaxation. Isatin inhibited the relaxation to ANP both in arteries with and without endothelium. Carotid arteries from diabetic rabbits showed a decreased natriuretic peptide receptor (NPR)-A expression and an enhanced NPR-C expression. Inhibition of NO-synthesis did not modify ANP-induced relaxation in control rabbits but inhibited it in diabetic rabbits. In arteries with endothelium indomethacin enhanced the relaxation to ANP in control rabbits but did not modify it in diabetic rabbits. In endothelium-denuded arteries indomethacin inhibited the relaxation to ANP in both groups of animals. In KCl-depolarised arteries, relaxation to ANP was almost abolished both in control and diabetic rabbits. Tetraethylammonium inhibited the relaxation to ANP, and this inhibition was higher in diabetic than in control rabbits. These results suggest that diabetes produces hyporeactivity of the rabbit carotid artery to ANP by a mechanism that at least includes a reduced expression of NPR-A, an enhanced expression of NPR-C and a reduced participation of K(+)-channels. Furthermore, diabetes enhances endothelial NO release and diminishes the ratio thromboxane A(2)/prostacyclin. This increase of vasodilators could result from compensatory mechanisms counteracting the arterial hyporeactivity to ANP.
authors.affiliation: Departamento de Fisiología, Facultad de Farmacia, Universidad de Valencia, Valencia, Spain.
authors.forename: Vannina G
authors.initials: VG
authors.lastname: Marrachelli
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Miranda
authors.forename: José M
authors.initials: JM
authors.lastname: Centeno
authors.forename: Ignacio
authors.initials: I
authors.lastname: Miranda
authors.forename: María
authors.initials: M
authors.lastname: Castelló-Ruiz
authors.forename: María C
authors.initials: MC
authors.lastname: Burguete
authors.forename: Teresa
authors.initials: T
authors.lastname: Jover-Mengual
authors.forename: Juan B
authors.initials: JB
authors.lastname: Salom
authors.forename: Germán
authors.initials: G
authors.lastname: Torregrosa
authors.forename: Enrique
authors.initials: E
authors.lastname: Alborch
mesh_terms: D000818:Animals; D009320:Atrial Natriuretic Factor / Q000502:physiology*; D002339:Carotid Arteries / Q000378:metabolism*; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism* / Q000503:physiopathology; D015536:Down-Regulation / Q000502:physiology*; D008297:Male; D011817:Rabbits; D011897:Random Allocation; D014664:Vasodilation / Q000502:physiology*
chemical_list: D009320:Atrial Natriuretic Factor