title: Preliminary characterization of a glucose-sensitive hydrogel.
abstract: We present proof-of-concept studies that display the potential for using a glucose-sensitive hydrogel as a continuous glucose sensor. A study to characterize the swelling ratio of the hydrogel at normal physiological and pathological hyperglycemic glucose levels was performed. The hydrogel exposed to the hyperglycemic glucose solution had a higher equilibrium swelling ratio than the hydrogel exposed to the normal glucose concentration solution. The diffusivity of a small molecule, fluorescein isothiocyanate (FITC), through a hydrogel exposed to a hyperglycemic solution was determined using fluorescence recovery after photobleaching (FRAP). The diffusivity was found to be 4.2 × 10(-14) m(2)/s, a value approximately four orders of magnitude smaller than the diffusivity of FITC in glucose solution. The permeability of the hydrogel after equilibration in a hyperglycemic solution was found to be 5.1 × 10(-17) m(2), in the range of 2-4% agarose gels.
authors.affiliation: Brain Computer Interface Laboratory and the Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907, USA. bbeier@purdue.edu
authors.forename: Brooke L
authors.initials: BL
authors.lastname: Beier
authors.forename: Eric M
authors.initials: EM
authors.lastname: Brandner
authors.forename: Katherine M
authors.initials: KM
authors.lastname: Musick
authors.forename: Akira
authors.initials: A
authors.lastname: Matsumoto
authors.forename: Alyssa
authors.initials: A
authors.lastname: Panitch
authors.forename: Eric A
authors.initials: EA
authors.lastname: Nauman
authors.forename: Pedro P
authors.initials: PP
authors.lastname: Irazoqui
mesh_terms: D001692:Biological Transport; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D005453:Fluorescence; D036681:Fluorescence Recovery After Photobleaching; D005947:Glucose / Q000032:analysis*; D020100:Hydrogels / Q000737:chemistry*; D013997:Time Factors
chemical_list: D020100:Hydrogels; D005947:Glucose