title: Fed-batch fermentation of GM-CSF-producing glycoengineered Pichia pastoris under controlled specific growth rate.
abstract: BACKGROUND
  Yeast expression systems with altered N-glycosylation are now available to produce glycoproteins with homogenous, defined N-glycans. However, data on the behaviour of these strains in high cell density cultivation are scarce.
  
  
  RESULTS
  Here, we report on cultivations under controlled specific growth rate of a GlycoSwitch-Man5 Pichia pastoris strain producing Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) at high levels (hundreds of milligrams per liter). We demonstrate that homogenous Man5GlcNAc2 N-glycosylation of the secreted proteins is achieved at all specific growth rates tested.
  
  
  CONCLUSIONS
  Together, these data illustrate that the GlycoSwitch-Man5 P. pastoris is a robust production strain for homogenously N-glycosylated proteins.
authors.affiliation: Unit for Molecular Glycobiology, Department for Molecular Biomedical Research, VIB, Ghent, Belgium. pjacobs@rics.bwh.harvard.edu
authors.forename: Pieter P
authors.initials: PP
authors.lastname: Jacobs
authors.forename: Mehmet
authors.initials: M
authors.lastname: Inan
authors.forename: Nele
authors.initials: N
authors.lastname: Festjens
authors.forename: Jurgen
authors.initials: J
authors.lastname: Haustraete
authors.forename: Annelies
authors.initials: A
authors.lastname: Van Hecke
authors.forename: Roland
authors.initials: R
authors.lastname: Contreras
authors.forename: Michael M
authors.initials: MM
authors.lastname: Meagher
authors.forename: Nico
authors.initials: N
authors.lastname: Callewaert
mesh_terms: D003001:Cloning, Molecular; D005285:Fermentation; D006031:Glycosylation; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D010843:Pichia / Q000254:growth & development* / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D011994:Recombinant Proteins; D016178:Granulocyte-Macrophage Colony-Stimulating Factor