title: The contribution of Raman scattering to the fluorescence of the polyene antibiotic amphotericin B.
abstract: Fluorescence has recently been applied to the analysis of the molecular organization state of the polyene antibiotic amphotericin B (AmB) in solution or in lipid membranes. The polyene chain of AmB monomer gives rise to two fluorescence emissions; S(1)(2(1)A(g)) → S(0)(1(1)A(g)) between 500 and 700 nm, S(2)(1(1)B(u)) → S(0)(1(1)A(g)) between 400 and 500 nm. However, Raman scattering might interfere with the S(2) → S(0) emission fluorescence due to the weak fluorescence quantum yield and close proximity to the exciting lines. In fact, we show here that a change in the excitation wavelength results in a shift of three emission bands, an effect which excludes their assignment to fluorescence. These bands originate from the water Raman at 3382 cm(-1)and AmB resonance Raman at 1556 and 1153 cm(-1). As a consequence, some former conclusions on the molecular organization state of AmB should be reconsidered.
authors.affiliation: ANBioPhy, Université Pierre et Marie Curie, Genopole campus 1, Bât Genavenir 8, 5 rue Henri Desbruères, 91030, Evry Cedex, France. jacques.bolard@upmc.fr
authors.forename: Jacques
authors.initials: J
authors.lastname: Bolard
authors.forename: Monique
authors.initials: M
authors.lastname: Chéron
authors.forename: John D
authors.initials: JD
authors.lastname: Cleary
authors.forename: Robert E
authors.initials: RE
authors.lastname: Kramer
mesh_terms: D000666:Amphotericin B / Q000737:chemistry*; D000900:Anti-Bacterial Agents / Q000737:chemistry*; D016477:Artifacts; D006863:Hydrogen-Ion Concentration; D011090:Polyenes / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013059:Spectrum Analysis, Raman; D014867:Water / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D011090:Polyenes; D014867:Water; D000666:Amphotericin B