title: Different distribution of Cav3.2 and Cav3.1 transcripts encoding T-type Ca(2+) channels in the embryonic heart of mice.
abstract: We investigated the distribution of T-type Ca(2+) channel mRNAs in the mouse embryonic heart. Cav3.2, but not Cav3.1, was expressed in the E8.5 embryonic heart along with cardiac progenitor markers (Nkx2.5, Tbx5, Isl-1) and contractile proteins (alpha and beta MHC). In the E10.5 heart, the distribution of Cav3.1 mRNA was confirmed in the AV-canal and overlapped with that of MinK or Tbx2. Cav3.2 mRNA was observed not only in the AV-canal but also in the outflow tract, along with MinK and Isl-1, indicating the expression of Cav3.2 in the secondary heart field. Thus, Cav3.2 may contribute to the development of the outflow tract from the secondary heart field in the embryonic heart, whereas Cav3.1 may be involved in the development of the cardiac conduction-system together with Cav3.2.
authors.affiliation: Division of Molecular Medicine and Therapeutics, Department of Multidisciplinary Internal Medicine, Faculty of Medicine, Tottori University, Yonago, Japan. einosuke@kc4.so-net.ne.jp
authors.forename: Einosuke
authors.initials: E
authors.lastname: Mizuta
authors.forename: Manabu
authors.initials: M
authors.lastname: Shirai
authors.forename: Keita
authors.initials: K
authors.lastname: Arakawa
authors.forename: Kyoko
authors.initials: K
authors.lastname: Hidaka
authors.forename: Junichiro
authors.initials: J
authors.lastname: Miake
authors.forename: Haruaki
authors.initials: H
authors.lastname: Ninomiya
authors.forename: Masahiko
authors.initials: M
authors.lastname: Kato
authors.forename: Chiaki
authors.initials: C
authors.lastname: Shigemasa
authors.forename: Yasuaki
authors.initials: Y
authors.lastname: Shirayoshi
authors.forename: Ichiro
authors.initials: I
authors.lastname: Hisatome
authors.forename: Takayuki
authors.initials: T
authors.lastname: Morisaki
mesh_terms: D000818:Animals; D001483:Base Sequence; D020747:Calcium Channels, T-Type / Q000235:genetics*; D002460:Cell Line; D017931:DNA Primers / Q000235:genetics; D058732:Embryoid Bodies / Q000378:metabolism; D053595:Embryonic Stem Cells / Q000378:metabolism; D005318:Fetal Heart / Q000378:metabolism; D006321:Heart / Q000196:embryology*; D006329:Heart Conduction System / Q000196:embryology / Q000378:metabolism; D051379:Mice; D009206:Myocardium / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014018:Tissue Distribution
chemical_list: C498197:Cacna1g protein, mouse; C504883:Cacna1h protein, mouse; D020747:Calcium Channels, T-Type; D017931:DNA Primers; D012333:RNA, Messenger