title: Introduction to calibration curves in bioanalysis.
abstract: Analytical standards are used in bioanalytical analysis for the quantitative measurement of large and small molecules in the pharmaceutical industry. This article will introduce the reader to the common, acceptable practices for the use of calibration curves in chromatographic and ligand-binding assays. The discussion presented is a combination of regulatory requirements and the authors' experience in performing bioanalysis. While all possible techniques are not covered, generally acceptable practices are described to serve as a starting point for novice practitioners.
authors.affiliation: Tandem Labs, A LabCorp Company, 115 Silvia Street, West Trenton, NJ 08628, USA. ed.brewer@tandemlabs.com
authors.forename: Edward
authors.initials: E
authors.lastname: Brewer
authors.forename: Ernest
authors.initials: E
authors.lastname: Bollin
mesh_terms: D001702:Biopharmaceutics / Q000379:methods* / Q000592:standards; D002138:Calibration / Q000592:standards; D002845:Chromatography / Q000379:methods / Q000592:standards; D008024:Ligands; D004364:Pharmaceutical Preparations / Q000032:analysis* / Q000592:standards; D011786:Quality Control; D014481:United States; D014486:United States Food and Drug Administration
chemical_list: D008024:Ligands; D004364:Pharmaceutical Preparations