title: Proteomics investigation of human platelets in healthy donors and cystic fibrosis patients by shotgun nUPLC-MSE and 2DE: a comparative study.
abstract: Platelets are of pathophysiological relevance in haemostasis, wound repair, inflammation and cardiovascular disease. We have shown that human platelets express a biologically active Cystic Fibrosis Transmembrane Conductance Regulator, which is dysfunctional in Cystic Fibrosis (CF) patients, and regulate platelet responses related to inflammation and its resolution. In order to further elucidate platelet involvement in CF inflammation, we pursued a comparative proteomic analysis of cells from healthy donors and CF patients, in association with a non-supervised comparative analysis of the Gene Ontology. Our results, showing changes in the integrin signalling in CF, support a pro-inflammatory profile of CF platelets.
authors.affiliation: IRCCS-S. Lucia Foundation, Rome, Italy. luisa.pieroni@uniroma2.it
authors.forename: Luisa
authors.initials: L
authors.lastname: Pieroni
authors.forename: Francesco
authors.initials: F
authors.lastname: Finamore
authors.forename: Maurizio
authors.initials: M
authors.lastname: Ronci
authors.forename: Domenico
authors.initials: D
authors.lastname: Mattoscio
authors.forename: Valeria
authors.initials: V
authors.lastname: Marzano
authors.forename: Stefano Levi
authors.initials: SL
authors.lastname: Mortera
authors.forename: Serena
authors.initials: S
authors.lastname: Quattrucci
authors.forename: Giorgio
authors.initials: G
authors.lastname: Federici
authors.forename: Mario
authors.initials: M
authors.lastname: Romano
authors.forename: Andrea
authors.initials: A
authors.lastname: Urbani
mesh_terms: D000595:Amino Acid Sequence; D001792:Blood Platelets / Q000737:chemistry / Q000378:metabolism*; D016022:Case-Control Studies; D002851:Chromatography, High Pressure Liquid; D003550:Cystic Fibrosis / Q000378:metabolism*; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000097:blood* / Q000235:genetics / Q000276:immunology; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006262:Health; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D020543:Proteome / Q000032:analysis / Q000737:chemistry; D040901:Proteomics / Q000379:methods*; D014019:Tissue Donors
chemical_list: D020543:Proteome; D019005:Cystic Fibrosis Transmembrane Conductance Regulator