title: Diet-induced impaired glucose tolerance and gestational diabetes in the dog.
abstract: Glucose metabolism was compared in dogs consuming a chow/meat diet throughout pregnancy (P group, n = 6) and dogs switched to a high-fat/high-fructose (HFF) diet during the 4th-5th gestational week (gestation ≃9 wk; P-HFF group; n = 6). An oral glucose tolerance test (OGTT; 0.9 g/kg) was administered in the 6th-7th gestational week, and a hyperinsulinemic [0-120 min: 1.8 pmol·kg(-1)·min(-1) (low insulin); 120-240 min: 9 pmol·kg(-1)·min(-1) (high insulin)] euglycemic clamp was performed the following week. Nonpregnant (NP) female dogs underwent OGTTs but not clamp studies. All P-HFF dogs exhibited impaired glucose tolerance (IGT) or gestational diabetes (GDM), but only one P dog had IGT. Insulin concentrations in P and P-HFF dogs were significantly lower than in NP dogs 30 and 60 min after the OGTT. Therefore, mean islet size and area were evaluated in P and NP dogs. These values did not differ between groups, and proliferating endocrine cells were rare in pregnancy. During exposure to high insulin, glucose infusion rate and hindlimb glucose uptake were ∼30% greater (P < 0.05) and net hepatic glucose output was more suppressed (-5.5 ± 6.1 vs. 7.8 ± 2.8 mg·100 g liver(-1)·min(-1), P < 0.05) in P than in P-HFF dogs. In conclusion, in the 2nd trimester the canine pancreas does not exhibit islet hypertrophy, hyperplasia, or neogenesis. Combined with the lack of pancreatic adaptation, a HFF diet during late pregnancy produces a canine model of IGT and GDM without hyperinsulinemia but exhibiting liver and muscle insulin resistance.
authors.affiliation: Dept. of Molecular Physiology & Biophysics, Vanderbilt Univ. School of Medicine, 702 Light Hall, Nashville, TN 37232-0615, USA. genie.moore@vanderbilt.edu
authors.forename: Mary Courtney
authors.initials: MC
authors.lastname: Moore
authors.forename: Renuka
authors.initials: R
authors.lastname: Menon
authors.forename: Katie C
authors.initials: KC
authors.lastname: Coate
authors.forename: Maureen
authors.initials: M
authors.lastname: Gannon
authors.forename: Marta S
authors.initials: MS
authors.lastname: Smith
authors.forename: Ben
authors.initials: B
authors.lastname: Farmer
authors.forename: Phillip E
authors.initials: PE
authors.lastname: Williams
mesh_terms: D000818:Animals; D016640:Diabetes, Gestational / Q000209:etiology* / Q000378:metabolism*; D004041:Dietary Fats / Q000009:adverse effects*; D019422:Dietary Sucrose / Q000009:adverse effects*; D004285:Dogs; D005260:Female; D005947:Glucose / Q000378:metabolism*; D005951:Glucose Tolerance Test; D007333:Insulin Resistance; D011247:Pregnancy
chemical_list: D004041:Dietary Fats; D019422:Dietary Sucrose; D005947:Glucose