title: Immunohistochemical detection of extrinsic and intrinsic mediators of apoptosis in porcine paraffin-embedded tissues.
abstract: Apoptosis is a strictly regulated mechanism of cell death that involves a complex network of biochemical pathways. Whether a cell undergoes apoptosis or not depends on a delicate balance of anti- and pro-apoptotic stimuli. This phenomenon can be induced by two different pathways: intrinsic and extrinsic pathways. The main aim of this study was to determine the ideal fixative and antigen retrieval method in porcine paraffin embedded tissues for the immunohistochemical detection of apoptosis mediators, from both extrinsic and intrinsic pathways. Tonsil, retropharyngeal lymph node and lung tissue samples were fixed in 10% neutral buffered formalin, Bouin solution and zinc salts fixative (ZSF) and different unmasking methods were carried out. Both 10% neutral buffered formalin and ZSF resulted as the fixatives of election to study apoptosis phenomena. Tween 20 (0.01% in PBS), citrate buffer (microwave, pH 6.0) and/or protease type XIV were the antigen retrieval methods which displayed better labelling. Our results allow to deep in the knowledge of apoptosis and its role in the pathogenesis of porcine diseases.
authors.affiliation: Department of Anatomy and Comparative Pathology, Faculty of Veterinary Medicine, Cordoba University, 14014 Cordoba, Spain.
authors.forename: Inmaculada
authors.initials: I
authors.lastname: Barranco
authors.forename: Jaime
authors.initials: J
authors.lastname: Gómez-Laguna
authors.forename: Irene M
authors.initials: IM
authors.lastname: Rodríguez-Gómez
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Salguero
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Pallarés
authors.forename: Antonio
authors.initials: A
authors.lastname: Bernabé
authors.forename: Librado
authors.initials: L
authors.lastname: Carrasco
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D015415:Biomarkers; D005786:Gene Expression Regulation; D007150:Immunohistochemistry / Q000379:methods / Q000662:veterinary*; D008168:Lung / Q000166:cytology / Q000378:metabolism*; D008198:Lymph Nodes / Q000166:cytology / Q000378:metabolism*; D014066:Palatine Tonsil / Q000166:cytology / Q000378:metabolism*; D016612:Paraffin Embedding / Q000662:veterinary*; D013048:Specimen Handling; D013552:Swine
chemical_list: D015415:Biomarkers