title: Characterization of TTAGG telomeric repeats, their interstitial occurrence and constitutively active telomerase in the mealybug Planococcus lilacinus (Homoptera; Coccoidea).
abstract: We confirmed the occurrence of the insect TTAGG telomeric repeats in the mealybug Planococcus lilacinus, a radiation-resistant coccid, by single primer polymerase chain reaction (PCR) and Southern hybridization. Analysis of Bal31 nuclease-digested DNA by Southern hybridization and chromosomes by FISH suggests that these repeats occur mainly at the ends of the chromosomes. However, sequence analysis of the PCR products of TTAGG-associated sequences from genomic DNA showed their interstitial occurrence and association with certain unrelated low-copy repeats. Because of their shorter length, the interstitial TTAGG sequences were detectable by primed in situ hybridizations but not by FISH. Analysis of chromosomes recovered after irradiation by fluorescent in situ hybridization suggested acquisition of TTAGG repeats at a majority of the healed ends. We also observed mild telomerase activity in unirradiated insects which was further enhanced after irradiation. Taken together, these results suggest that the mealybug has an efficient mechanism of formation of TTAGG repeats at radiation-induced chromosome ends and constitutively active telomerase may be a feature associated with rapid recovery of chromosome ends damaged by ionizing radiation.
authors.affiliation: Centre for Human Genetics, Biotech Park, Next to Aravind Mills, Bangalore 560100, India. kommumohan@gmail.com
authors.forename: Kommu Naga
authors.initials: KN
authors.lastname: Mohan
authors.forename: B Sandya
authors.initials: BS
authors.lastname: Rani
authors.forename: Pooja Swaroop
authors.initials: PS
authors.lastname: Kulashreshta
authors.forename: Jayarama S
authors.initials: JS
authors.lastname: Kadandale
mesh_terms: D000818:Animals; D001483:Base Sequence; D059006:Chromosomes, Insect / Q000235:genetics / Q000528:radiation effects; D005260:Female; D006430:Hemiptera / Q000201:enzymology* / Q000235:genetics* / Q000528:radiation effects; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008969:Molecular Sequence Data; D019098:Telomerase / Q000235:genetics / Q000378:metabolism*; D016615:Telomere / Q000235:genetics*; D020079:Terminal Repeat Sequences
chemical_list: D019476:Insect Proteins; D019098:Telomerase