title: [Electricity generation and quinoline degradation of pure strains and mixed strains in the microbial fuel cell].
abstract: Microbial flora composition of microbial fuel cells (MFC) is important to the electricity generation. Four bacterium strains Q1, b, c and d which represent all different morphology of culturable bacterium were isolated from a MFC using 200 mg x L(-1) quinoline as the fuel and operating for at least 210 days. Strains Q1, c and d were Pseudomonas sp. based on 16S rDNA sequence analysis, while strain b was Burkholderia sp. Double-chamber MFCs using 200 mg x L(-1) quinoline and 300 mg x L(-1) glucose as the fuel and potassium ferricyanide as the electron acceptor were constructed. Results showed that strain b, c and d were non-electrogenesis. The electrical charges of MFC inoculated electrogenesis strain Q1 with non-electrogenesis strain b, c and d respectively were 3.00, 3.57 and 5.13C, and the columbic efficiency were 3.85%, 4.59% and 6.58%, which were all lower than that inoculated with pure Q1, because of the interspecific competition of electrogenesis and non-electrogenesis bacteria. Combinations of Q1 with the other three strains respectively resulted in 100% of quinoline degradation rates within 24h, which is better than pure cultures, that is, mixed microbial populations perform better in MFC when complex organics are used as the fuel. GC/MS analyses showed that only 2(1H)-quinolinone and phenol existed in the effluent of the MFC, which was inoculated with only Q1 or mixed bacteria.
authors.affiliation: School of Environmental Science and Engineering, Sun Yat-sen University, Guangzhou 510275, China. saandpi@yahoo.com.cn
authors.forename: Shan-Shan
authors.initials: SS
authors.lastname: Chen
authors.forename: Cui-Ping
authors.initials: CP
authors.lastname: Zhang
authors.forename: Guang-Li
authors.initials: GL
authors.lastname: Liu
authors.forename: Ren-Duo
authors.initials: RD
authors.lastname: Zhang
authors.forename: Ming-Chen
authors.initials: MC
authors.lastname: Li
authors.forename: Xiang-Chun
authors.initials: XC
authors.lastname: Quan
mesh_terms: D001419:Bacteria / Q000378:metabolism*; D001673:Biodegradation, Environmental; D001674:Bioelectric Energy Sources / Q000382:microbiology*; D019117:Burkholderia / Q000378:metabolism; D004560:Electricity; D004563:Electrochemistry; D004566:Electrodes / Q000382:microbiology; D005947:Glucose / Q000378:metabolism; D010084:Oxidation-Reduction; D011549:Pseudomonas / Q000378:metabolism; D011804:Quinolines / Q000378:metabolism*
chemical_list: D011804:Quinolines; C037219:quinoline; D005947:Glucose