title: Intragastric self-infusion of ethanol in high- and low-drinking mouse genotypes after passive ethanol exposure.
abstract: Two experiments examined the effect of 5 days of passive exposure to ethanol (or water) on later self-infusion of ethanol or water via surgically implanted intragastric (IG) catheters in mouse genotypes previously shown to drink high (C57BL/6J, HAP2) or low (DBA/2J, LAP2) amounts of ethanol in home-cage continuous-access two-bottle choice procedures. Intragastric ethanol self-infusion was affected by both genotype and a history of passive ethanol exposure, with greater intakes in the high-drinking genotypes and in groups that received passive exposure to ethanol. Passive ethanol exposure also increased preference for the flavor that signaled ethanol infusion (S+), eliminating genetic differences in this measure. The increases in ethanol intake and S+ preference induced by ethanol exposure might have been mediated jointly by development of tolerance to aversive post-absorptive ethanol effects and negative reinforcement because of alleviation of withdrawal. Bout analyses indicated that ethanol exposure increased ethanol self-infusion by increasing the total number of daily bouts rather than by increasing bout size. These analyses also showed that DBA/2J mice infused larger ethanol bouts and a greater percentage of their total intakes in large bouts than C57BL/6J mice. Overall, these studies suggest that the IG self-infusion procedure is a potentially useful new tool for studying genetic and environmental influences on excessive ethanol intake and preference in mice.
authors.affiliation: Department of Behavioral Neuroscience and Portland Alcohol Research Center, Oregon Health & Science University, Portland, OR 97239-3098, USA.
authors.forename: T L
authors.initials: TL
authors.lastname: Fidler
authors.forename: A M
authors.initials: AM
authors.lastname: Dion
authors.forename: M S
authors.initials: MS
authors.lastname: Powers
authors.forename: J J
authors.initials: JJ
authors.lastname: Ramirez
authors.forename: J A
authors.initials: JA
authors.lastname: Mulgrew
authors.forename: P J
authors.initials: PJ
authors.lastname: Smitasin
authors.forename: A T
authors.initials: AT
authors.lastname: Crane
authors.forename: C L
authors.initials: CL
authors.lastname: Cunningham
mesh_terms: D058956:Administration, Mucosal; D000428:Alcohol Drinking / Q000235:genetics*; D020268:Alcohol-Induced Disorders, Nervous System / Q000235:genetics*; D000437:Alcoholism / Q000235:genetics*; D000818:Animals; D002492:Central Nervous System Depressants / Q000378:metabolism / Q000494:pharmacology; D004195:Disease Models, Animal; D004333:Drug Administration Routes; D000431:Ethanol / Q000378:metabolism / Q000494:pharmacology*; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D012646:Self Administration / Q000379:methods; D013045:Species Specificity
chemical_list: D002492:Central Nervous System Depressants; D000431:Ethanol