title: Requirement of regulated endocrine-specific protein-18 for development and expression of regulated endocrine-specific protein-18 isoform c in mice.
abstract: Regulated endocrine-specific protein-18 (RESP18) is distributed mainly in the peripheral endocrine and neuroendocrine tissues. The expression of RESP18 protein is regulated by physiological factors, such as blood glucose or dopaminergic drugs, but its functions remain unclear. In this study, to explore the biological functions of RESP18 in vivo, we generated RESP18 heterozygous deficient mice, and further found RESP18 was essential for embryonic development. In addition, we cloned a new isoform of mouse RESP18 by reverse transcription-polymerase chain reaction (RT-PCR), and denominated it as RESP18-c. Mouse RESP18-c, by skipping exon4 (43 bp in length), encodes a shorter protein of 120 amino acid residues. The distribution of RESP18-c mRNA is similar with that of RESP18 mRNA in the peripheral tissues and brains of mice.
authors.affiliation: State Key Laboratory of Medical Neurobiology, Shanghai Medical College, Fudan University, 138 Yixueyuan Road, Shanghai, 200032, People's Republic of China.
authors.forename: Min
authors.initials: M
authors.lastname: Liang
authors.forename: Jian Ling
authors.initials: JL
authors.lastname: Yang
authors.forename: Min Juan
authors.initials: MJ
authors.lastname: Bian
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
authors.forename: Xiao Qi
authors.initials: XQ
authors.lastname: Hong
authors.forename: Yan Cong
authors.initials: YC
authors.lastname: Wang
authors.forename: Yu Fang
authors.initials: YF
authors.lastname: Huang
authors.forename: Shu Ping
authors.initials: SP
authors.lastname: Gu
authors.forename: Mei
authors.initials: M
authors.lastname: Yu
authors.forename: Fang
authors.initials: F
authors.lastname: Huang
authors.forename: Jian
authors.initials: J
authors.lastname: Fei
mesh_terms: D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D004622:Embryo, Mammalian / Q000378:metabolism*; D047108:Embryonic Development / Q000235:genetics / Q000502:physiology*; D004703:Endocrine System / Q000378:metabolism*; D018390:Gene Targeting / Q000379:methods; D051379:Mice; D008817:Mice, Mutant Strains; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D020033:Protein Isoforms / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D009419:Nerve Tissue Proteins; D020033:Protein Isoforms; C086381:regulated endocrine secretory protein 18