title: Water-solubilisation and bio-conjugation of a red-emitting BODIPY marker.
abstract: The synthesis and preliminary bio-conjugation studies of a novel water-soluble red-emitting di-styryl BODIPY dye are disclosed. Aggregation behaviour of this compound under physiological conditions was suppressed by specific introduction of a di-sulfonated peptide-based linker at the meso phenyl substituent, sultonated styryl arms and short polyethyleneglycol chains at the boron center. Thus, a good quantum yield of 22% in PBS for this red-emitting BODIPY was obtained. Introduction of an activated ester function enabled successful bio-conjugation to monoclonal antibodies and proteins.
authors.affiliation: LCOSA, ECPM, UMR 7515, CNRS-UdS, 25 rue Becquerel, 67087, Strasbourg Cedex 02, France.
authors.forename: Song Lin
authors.initials: SL
authors.lastname: Niu
authors.forename: Cédrik
authors.initials: C
authors.lastname: Massif
authors.forename: Gilles
authors.initials: G
authors.lastname: Ulrich
authors.forename: Raymond
authors.initials: R
authors.lastname: Ziessel
authors.forename: Pierre-Yves
authors.initials: PY
authors.lastname: Renard
authors.forename: Anthony
authors.initials: A
authors.lastname: Romieu
mesh_terms: D000478:Alkylation; D001896:Boron Compounds / Q000737:chemistry*; D003116:Color; D004151:Dipeptides / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D012995:Solubility; D014867:Water / Q000737:chemistry*
chemical_list: C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D004151:Dipeptides; D005456:Fluorescent Dyes; D014867:Water