title: [Expression of COX-2 in different human prostate cancer cell lines and its significance].
abstract: OBJECTIVE
  To investigate the expression of cyclooxygenase-2 (COX-2) in different prostate cancer (PCa) cell lines and its role in the acquisition of invasive and metastatic potentials of PCa.
  
  
  METHODS
  We detected the expressions of COX-2 in prostate cancer cell lines LNCaP, C4-2, IF11, IA8 and PC-3 with different metastatic potentials by Western blotting and RT-PCR, and analyzed their roles in the invasion and metastasis of different PCa cell lines.
  
  
  RESULTS
  Western blotting and RT-PCR showed that the expression of the COX-2 protein was high in PC-3, but absent in IF11, IA8, LNCaP and C4-2 (P < 0.05), and it was consistent with the expression of COX-2 mRNA.
  
  
  CONCLUSIONS
  COX-2 expresses differently in PCa cell lines with different metastatic potentials. The overexpression of COX-2 may be associated with the high invasion and metastasis of PC-3, but not with the metastasis of other cell lines.
authors.affiliation: Department of Urology, Beijing Anzhen Hospital Affiliated to Capital Medical University, Beijing 100029, China.
authors.forename: Jia-Hui
authors.initials: JH
authors.lastname: Zhao
authors.forename: Yong-Guang
authors.initials: YG
authors.lastname: Jiang
authors.forename: Chun-Ting
authors.initials: CT
authors.lastname: Wu
authors.forename: Yong
authors.initials: Y
authors.lastname: Luo
authors.forename: Zhu
authors.initials: Z
authors.lastname: Hou
authors.forename: Da-Lin
authors.initials: DL
authors.lastname: He
mesh_terms: D045744:Cell Line, Tumor; D051546:Cyclooxygenase 2 / Q000378:metabolism*; D006801:Humans; D008297:Male; D009362:Neoplasm Metastasis; D011471:Prostatic Neoplasms / Q000378:metabolism* / Q000473:pathology*; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human