title: [Effect of ischemia preconditioning on pro- and anti- angiogenic molecule expression and functional arteries formation].
abstract: OBJECTIVE
  to observe the effect of ischemia preconditioning (IPC) on the expression of pro-angiogenic VEGF, PDGF and anti-angiogenic ADAMTS-1, and arteriogenesis.
  
  
  METHODS
  rat heart IPC model was made by 4 circles of occluding the LAD for 6 min followed by 6 min of reperfusion. The expression of VEGF, PDGF-B and ADAMTS-1 in the ischemic area was examined with immunohistochemistry at 6, 12 and 24 h after IPC. IPC plus myocardial infarction model was induced by LAD ligation 24 h after IPC, 14 days later, the anti-SM-α-actin antibody was used to detect the mature neovascularization in the border of the infracted area.
  
  
  RESULTS
  VEGF, PDGF-B and ADAMTS-1 were significantly upregulated in the ischemic area in IPC group compared with the control group (P < 0.05). Density of mature arteries was also significantly increased in IPC plus MI group than that in MI group (P < 0.05).
  
  
  CONCLUSIONS
  IPC promoted the formation of mature new arteries which may be modulated by upregulating VEGF, PDGF-B, and ADAMTS-1 expressions.
authors.affiliation: Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan 430060, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: WANG
authors.forename: Cong-xin
authors.initials: CX
authors.lastname: HUANG
authors.forename: Jin-song
authors.initials: JS
authors.lastname: CHENG
authors.forename: Hui
authors.initials: H
authors.lastname: WANG
authors.forename: Wen-jing
authors.initials: WJ
authors.lastname: WU
authors.forename: Wen-qiang
authors.initials: WQ
authors.lastname: LIAO
authors.forename: Jian-yan
authors.initials: JY
authors.lastname: WEN
authors.forename: Yuan-nan
authors.initials: YN
authors.lastname: KE
authors.forename: Jin-gang
authors.initials: JG
authors.lastname: ZHENG
mesh_terms: D051722:ADAM Proteins / Q000378:metabolism; D000071097:ADAMTS1 Protein; D000818:Animals; D001158:Arteries / Q000378:metabolism* / Q000473:pathology; D019194:Ischemic Preconditioning; D008297:Male; D018919:Neovascularization, Physiologic; D020574:Proto-Oncogene Proteins c-sis / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015854:Up-Regulation; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*
chemical_list: D020574:Proto-Oncogene Proteins c-sis; D042461:Vascular Endothelial Growth Factor A; C467498:vascular endothelial growth factor A, rat; D051722:ADAM Proteins; D000071097:ADAMTS1 Protein; C486324:Adamts1 protein, rat