title: [The nuclear matrix proteins (mol. mass 38 and 50 kDa) are transported by chromosomes in mitosis].
abstract: It was shown by immunofluorescence method that serum M68 and serum K43 from patients with autoimmune disease stain interphase nuclei and periphery of mitotic chromosomes of pig kidney cells. Western blotting reveals the polypeptide with mol. mass of 50 kDa in serum M68, and the polypeptide with mol. mass of 38 kDa in serum K43. In the nuclear protein matrix, the antibodies to protein with mol. mass of 38 kDa stained only nucleolar periphery, while the antibodies to the protein with mol. mass of 50 kDa stained both the nucleolar periphery and all the interphase nucleus. It shows that among all components of nuclear protein matrix (lamina, internuclear network, residual nucleoli) only nucleolar periphery contains the 38 kDa protein, while the 50 kDa protein is a part of residual nucleolar periphery and takes part in nuclear protein network formation. In the interphase cells, both proteins were in situ localized in the nuclei, but one of them with mol. mass of 50 kDa was in the form of small clearly outlined granules, while the other (38 kDa) was in the form of small bright granules against the background of diffusely stained nuclei. Both proteins were also revealed as continuous ring around nucleolar periphery. During all mitotic stages, the 50 kDa protein was seen on the chromosomal periphery as a cover, and the 38 kDa protein formed separate fragments and granules around them. After nuclear and chromosome decondensation induced by hypotonic treatment, both antibodies stain interphase nuclei in diffuse manner, but in mitotic cells they stained the surface of the swollen chromosomes. The polypeptide with mol. mass of 50 kDa maintained strong connection with chromosome periphery both in norm and under condition of decondensation induced by hypotonic treatment and at subsequent recondensation in isotonic medium. In contrast, the protein with mol. mass of 38 kDa partially lost the contact with a chromosome during recondensation appearing also in the form of granules in cytoplasm. The data allow to consider, that nuclear matrix proteins can be transported as a part of peripheral chromosomal material, and that they can have connection of different stability with chromosomal periphery as well as the main nucleolar proteins (fibrillarin, B-23, nucleolin et al.) and some non-nucleolar components of nuclear protein matrix.
authors.forename: M I
authors.initials: MI
authors.lastname: Murasheva
authors.forename: Iu S
authors.initials: IuS
authors.lastname: Chentsov
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D033481:Chromosomes, Mammalian / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D008938:Mitosis; D008970:Molecular Weight; D034521:Nuclear Matrix-Associated Proteins / Q000737:chemistry / Q000378:metabolism*; D013552:Swine
chemical_list: D034521:Nuclear Matrix-Associated Proteins