title: MICER targeting vectors for manipulating the mouse genome.
abstract: The mouse has become an important model for understanding human development, physiology and disease because of its genetic and biological similarity to humans. Desired mouse mutants with precise genetic alterations can now be generated through gene targeting in mouse embryonic stem cells. The rate-limiting factor in a gene-targeting experiment is the time needed for cloning to construct targeting vectors. The establishment of the Mutagenic Insertion and Chromosome Engineering Resource has made available targeting vectors for the insertional mutagenesis of a large number of mouse genes as well as for chromosome engineering throughout the mouse genome. This unique resource has enriched the repertoire of the genetic reagents for targeted manipulation of the mouse genome.
authors.affiliation: Genetics Program and Department of Cancer Genetics, Roswell Park Cancer Institute, New York State Center of Excellence in Bioinformatics and Life Sciences Buffalo, New York, NY, USA.
authors.forename: Chunhong
authors.initials: C
authors.lastname: Liu
authors.forename: Paul F
authors.initials: PF
authors.lastname: Szurek
authors.forename: Y Eugene
authors.initials: YE
authors.lastname: Yu
mesh_terms: D000818:Animals; D018390:Gene Targeting / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics*; D051379:Mice; D008822:Mice, Transgenic; D008957:Models, Genetic; D016254:Mutagenesis, Insertional / Q000379:methods*