title: Rapid DNA detection by interface PCR on nanoparticles.
abstract: A novel, rapid DNA detection method based on fluorescence quenching of quantum dots (QDs) by gold nanoparticles (GNPs) through polymerase chain reaction (PCR) was developed. In proof-of-concept experiments, the length of the amplicon DNA ranging from 152 to 1003 base pairs (bp) could be determined based on quenched fluorescence intensity with 136 bp as the lower limit of effective range. And the real sample detections were also achieved successfully by this developed method. Therefore, this DNA detection method has the potential to be the powerful gene diagnostic tool.
authors.affiliation: School of Food Science and Technology, State Key Lab of Food Science and Technology, Jiangnan University, Wuxi, JiangSu, 214122, PR China.
authors.forename: Hua
authors.initials: H
authors.lastname: Kuang
authors.forename: Shuge
authors.initials: S
authors.lastname: Zhao
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.forename: Wei
authors.initials: W
authors.lastname: Ma
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Yong
authors.forename: Liguang
authors.initials: L
authors.lastname: Xu
authors.forename: Libing
authors.initials: L
authors.lastname: Wang
authors.forename: Chuanlai
authors.initials: C
authors.lastname: Xu
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D004247:DNA / Q000032:analysis / Q000235:genetics*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006046:Gold / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry*; D016133:Polymerase Chain Reaction / Q000295:instrumentation*; D045663:Quantum Dots; D017422:Sequence Analysis, DNA / Q000295:instrumentation*; D013050:Spectrometry, Fluorescence / Q000295:instrumentation*
chemical_list: D006046:Gold; D004247:DNA