title: Transmissible gastroenteritis virus (TGEV)-based vectors with engineered murine tropism express the rotavirus VP7 protein and immunize mice against rotavirus.
abstract: A coronavirus vector based on the genome of the porcine transmissible gastroenteritis virus (TGEV) expressing the rotavirus VP7 protein was constructed to immunize and protect against rotavirus infections in a murine model. The tropism of this TGEV-derived vector was modified by replacing the spike S protein with the homologous protein from mouse hepatitis virus (MHV). The rotavirus gene encoding the VP7 protein was cloned into the coronavirus cDNA. BALB/c and STAT1-deficient mice were inoculated with the recombinant viral vector rTGEV(S-MHV)-VP7, which replicates in the intestine and spreads to other organs such as liver, spleen and lungs. TGEV-specific antibodies were detected in all the inoculated BALB/c mice, while rotavirus-specific antibodies were found only after immunization by the intraperitoneal route. Partial protection against rotavirus-induced diarrhea was achieved in suckling BALB/c mice born to dams immunized with the recombinant virus expressing VP7 when they were orally challenged with the homotypic rotavirus strain.
authors.affiliation: Department of Microbiology and Ecology, School of Medicine, University of Valencia, Avda. Blasco Ibáñez, 17, 46010 Valencia, Spain.
authors.forename: Juan Manuel
authors.initials: JM
authors.lastname: Ribes
authors.forename: Javier
authors.initials: J
authors.lastname: Ortego
authors.forename: Juan
authors.initials: J
authors.lastname: Ceriani
authors.forename: Rebeca
authors.initials: R
authors.lastname: Montava
authors.forename: Luis
authors.initials: L
authors.lastname: Enjuanes
authors.forename: Javier
authors.initials: J
authors.lastname: Buesa
mesh_terms: D000818:Animals; D000833:Animals, Suckling; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology; D000956:Antigens, Viral / Q000235:genetics*; D036022:Capsid Proteins / Q000235:genetics*; D002460:Cell Line; D005260:Female; D015967:Gene Expression Regulation, Viral; D005818:Genetic Engineering; D007112:Immunity, Maternally-Acquired; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D012367:RNA, Viral; D012400:Rotavirus Infections / Q000517:prevention & control*; D022243:Rotavirus Vaccines; D050794:STAT1 Transcription Factor; D013552:Swine; D005760:Transmissible gastroenteritis virus; D014779:Virus Replication
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D036022:Capsid Proteins; D012367:RNA, Viral; D022243:Rotavirus Vaccines; D050794:STAT1 Transcription Factor; C494080:Stat1 protein, mouse; C050779:VP7 protein, Rotavirus