title: Cell-free miR-24 and miR-30d, potential diagnostic biomarkers in malignant effusions.
abstract: OBJECTIVE
  The study was trying to figure out the possible role of cell-free miR-24 and miR-30d as diagnostic biomarkers for malignant effusions.
  
  
  METHODS
  A total of 110 effusion samples (28 benign and 82 malignant) were collected and cell-free total RNA was extracted. Quantitative RT-PCR was carried out to measure the expression levels of miR-24 and miR-30d, with an exogenous supplemented plant miRNA, ath-miR156a as the reference RNA.
  
  
  RESULTS
  Malignant pleural effusions had higher expression levels of cell-free miR-24 and miR-30d than benign effusions. In the ascites group, only miR-30d was found to be significantly up-regulated in the supernatant of effusions. Combination of cell-free miR-24 and miR-30d could discriminate malignant ascites from benign with improved potency comparing to single miR-30d.
  
  
  CONCLUSIONS
  Cell-free miR-30d and miR-24 are potential diagnostic biomarkers for malignant and benign effusions.
authors.affiliation: The Comprehensive Cancer Center of Drum Tower Hospital affiliated to Medical School of Nanjing University & Clinical Cancer Institute of Nanjing University, Nanjng 21008, PR China; Jiangsu Key Laboratory for Molecular Medicine, Nanjing 210008, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Xie
authors.forename: Tingting
authors.initials: T
authors.lastname: Wang
authors.forename: Shaorong
authors.initials: S
authors.lastname: Yu
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.forename: Lifeng
authors.initials: L
authors.lastname: Wang
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Qian
authors.forename: Lixia
authors.initials: L
authors.lastname: Yu
authors.forename: Yitao
authors.initials: Y
authors.lastname: Ding
authors.forename: Chenyu
authors.initials: C
authors.lastname: Zhang
authors.forename: Baorui
authors.initials: B
authors.lastname: Liu
mesh_terms: D001201:Ascites; D014408:Biomarkers, Tumor / Q000235:genetics; D035683:MicroRNAs / Q000235:genetics; D016066:Pleural Effusion, Malignant / Q000175:diagnosis; D015854:Up-Regulation
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs