title: Chemometric-assisted kinetic-spectrophotometric method for simultaneous determination of ascorbic acid, uric acid, and dopamine.
abstract: A chemometric-assisted kinetic spectrophotometric method has been developed for simultaneous determination of ascorbic acid (AA), uric acid (UA), and dopamine (DA). This method relies on the difference in the kinetic rates of the reactions of analytes with a common oxidizing agent, tris(1,10-phenanthroline) and iron(III) complex (ferritin, [Fe(phen)(3)](3+)) at pH 4.4. The changes in absorbance were monitored spectrophotometrically. The data obtained from the experiments were processed by chemometric methods of artificial neural network (ANN) and partial least squares (PLS). Acceptable techniques of prediction set, randomization t test, cross-validation, and Y randomization were applied for the selection of the best chemometric method. The results showed that feedforward artificial neural network (FFANN) is more efficient than the other chemometric methods. The parameters affecting the experimental conditions were optimized, and it was found that under optimal conditions Beer's law is followed in the concentration ranges of 4.3-74.1, 4.3-78.3, and 2.0-33.0 μM for AA, UA, and DA, respectively. The proposed method was successfully applied to the determination of analytes in serum and urine samples.
authors.affiliation: Department of Chemistry, Faculty of Science, Yazd University, Yazd 89195-741, Iran.
authors.forename: Masoud Rohani
authors.initials: MR
authors.lastname: Moghadam
authors.forename: Shayessteh
authors.initials: S
authors.lastname: Dadfarnia
authors.forename: Ali Mohammad Haji
authors.initials: AM
authors.lastname: Shabani
authors.forename: Parviz
authors.initials: P
authors.lastname: Shahbazikhah
mesh_terms: D000042:Absorption; D001205:Ascorbic Acid / Q000032:analysis*; D002138:Calibration; D004298:Dopamine / Q000032:analysis*; D004563:Electrochemistry / Q000379:methods*; D005290:Ferric Compounds / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D015999:Multivariate Analysis; D016571:Neural Networks, Computer; D010618:Phenanthrolines / Q000737:chemistry; D044967:Serum / Q000737:chemistry; D013053:Spectrophotometry / Q000379:methods*; D014527:Uric Acid / Q000032:analysis*; D014556:Urine / Q000737:chemistry
chemical_list: D005290:Ferric Compounds; D010618:Phenanthrolines; D014527:Uric Acid; D001205:Ascorbic Acid; D004298:Dopamine; C025205:1,10-phenanthroline