title: Protective effects of quercetin on testicular torsion/detorsion-induced ischaemia-reperfusion injury in rats.
abstract: The aim of this study was to investigate the protective effect of quercetin (QE) on testicular torsion/detorsion-induced ischaemia-reperfusion (I/R) injury. A total of 24 male Wistar albino rats were divided into three groups: control, I/R and I/R treated with QE; each group contain eight animals. Testicular torsion was created by rotating the left testis 720° in a clockwise direction. The ischaemia period was 5 h and orchiectomy was performed after 5 h of detorsion. QE (15 mg kg(-1) , i.p.) was administered only once, 40 min prior to detorsion. Left orchiectomy was performed in all I/R groups. To date, no histopathological changes on testicular torsion/detorsion-induced I/R injury in rats by QE treatment have been reported. Spermatogenesis and mean seminiferous tubule diameter were significantly decreased in I/R groups were compared with the control group. Furthermore, QE treated animals showed an improved histological appearance in I/R group. Our data indicate a significant reduction in the activity of TUNEL, endothelial nitric oxide synthase and a rise in the expression of testosterone in testes tissue of I/R treated with QE therapy. We believe that further preclinical research into the utility of QE may indicate its usefulness as a potential treatment on testes injury after I/R in rats.
authors.affiliation: Department of Urology, Faculty of Medicine, University of Trakya, Edirne, Turkey.
authors.forename: T
authors.initials: T
authors.lastname: Aktoz
authors.forename: M
authors.initials: M
authors.lastname: Kanter
authors.forename: C
authors.initials: C
authors.lastname: Aktas
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D008297:Male; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism; D009919:Orchiectomy; D011794:Quercetin / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000473:pathology; D013086:Spermatic Cord Torsion / Q000150:complications / Q000188:drug therapy* / Q000473:pathology; D013737:Testis / Q000187:drug effects* / Q000473:pathology; D013739:Testosterone / Q000378:metabolism
chemical_list: D013739:Testosterone; D011794:Quercetin; D052250:Nitric Oxide Synthase Type III