title: Uranium bioaccumulation and biological disorders induced in zebrafish (Danio rerio) after a depleted uranium waterborne exposure.
abstract: Because of its toxicity and its ubiquity within aquatic compartments, uranium (U) represents a significant hazard to aquatic species such as fish. In a previous study, we investigated some biological responses in zebrafish either exposed to depleted or to enriched U (i.e., to different radiological activities). However, results required further experiments to better understand biological responses. Moreover, we failed to clearly demonstrate a significant relationship between biological effects and U radiological activity. We therefore chose to herein examine U bioaccumulation and induced effects in zebrafish according to a chemical dose-response approach. Results showed that U is highly bioconcentrated in fish, according to a time- and concentration-dependent model. Additionally, hepatic antioxidant defenses, red blood cells DNA integrity and brain acetylcholinesterase activity were found to be significantly altered. Generally, the higher the U concentration, the sooner and/or the greater the effect, suggesting a close relationship between accumulation and effect.
authors.affiliation: Laboratory of Radioecology and Ecotoxicology, IRSN (Institute for Radiological protection and Nuclear Safety), DEI/SECRE/LRE, Cadarache, Bat 186, BP 3, 13115 St-Paul-Lez-Durance cedex, France. sabrina.barillet@free.fr
authors.forename: Sabrina
authors.initials: S
authors.lastname: Barillet
authors.forename: Christelle
authors.initials: C
authors.lastname: Adam-Guillermin
authors.forename: Olivier
authors.initials: O
authors.lastname: Palluel
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Porcher
authors.forename: Alain
authors.initials: A
authors.lastname: Devaux
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000201:enzymology; D004249:DNA Damage / Q000187:drug effects; D004784:Environmental Monitoring / Q000379:methods*; D005260:Female; D029941:Fish Proteins / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D014501:Uranium / Q000378:metabolism / Q000633:toxicity*; D014875:Water Pollutants, Radioactive / Q000378:metabolism / Q000633:toxicity*; D015027:Zebrafish / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*
chemical_list: D029941:Fish Proteins; D014875:Water Pollutants, Radioactive; D014501:Uranium; D000110:Acetylcholinesterase