title: Cryopreservation of blastocysts using a modification of a simplified freezing protocol with a one step removal of cryoprotectant successfully used previously to freeze 2 pronuclear or multi-cell embryos.
abstract: OBJECTIVE
  To describe a modification of a simplified freezing protocol for the cryopreservation of blastocysts.
  
  
  METHODS
  1.5 M glycerol was substituted as a cryoprotectant instead of propanediol.
  
  
  RESULTS
  There was a survival rate of 59.1% (13/22) with three live deliveries in seven transfers (42.9% per transfer). The implantation rate was 28.6% (4/14).
  
  
  CONCLUSIONS
  This is the first description of a new technique for freezing blastocysts. A larger series is needed to determine if the good pregnancy rates will continue.
authors.affiliation: The University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School at Camden, Cooper Hospital/University Medical Center, Department of Obstetrics and Gynecology, Division of Reproductive Endocrinology & Infertility, Camden, NJ, USA.
authors.forename: D
authors.initials: D
authors.lastname: Summers-Chase
authors.forename: J H
authors.initials: JH
authors.lastname: Check
authors.forename: D
authors.initials: D
authors.lastname: Horwath
authors.forename: W
authors.initials: W
authors.lastname: Yuan
authors.forename: K
authors.initials: K
authors.lastname: Swenson
authors.forename: M
authors.initials: M
authors.lastname: Press
mesh_terms: D001755:Blastocyst; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents; D006801:Humans
chemical_list: D003451:Cryoprotective Agents