title: High sensitivity and selectivity of human antibody attachment at the interstices between substrate-bound gold nanoparticles.
abstract: Durable Au-patterning nanostructures with uniform spacing having an average nanoparticle size of 8 nm and an interparticle gap of 11 nm were fabricated using a microwave-plasma oven. Single molecules of immunoglobulin G (IgG) are individually occupied at the space constraints so that the extinction response of localized surface plasmon resonance (LSPR) can be observed when detecting human anti-IgG molecules, affording a detection limit of 66.7 pM.
authors.affiliation: Department of Chemistry National Chung Hsing University, Taichung 402, Taiwan. kjlin@dragon.nchu.edu.tw.
authors.forename: Chuen-Yuan
authors.initials: CY
authors.lastname: Hsu
authors.forename: Jing-Wen
authors.initials: JW
authors.lastname: Huang
authors.forename: Kuan-Jiuh
authors.initials: KJ
authors.lastname: Lin
mesh_terms: D000888:Antibodies, Anti-Idiotypic / Q000032:analysis* / Q000276:immunology; D055910:Antibodies, Immobilized / Q000737:chemistry* / Q000276:immunology; D006046:Gold / Q000737:chemistry*; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry* / Q000276:immunology; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D020349:Surface Plasmon Resonance / Q000379:methods*
chemical_list: D000888:Antibodies, Anti-Idiotypic; D055910:Antibodies, Immobilized; D007074:Immunoglobulin G; C048844:anti-IgG; D006046:Gold