title: Estrogens regulate the expression of NHERF1 in normal colon during the reproductive cycle of Wistar rats.
abstract: In breast cancer cell lines, the Na(+)/H(+) exchanger regulator factor 1 (NHERF1) gene is regulated at the transcriptional level by estrogens, the protein expression levels correlate with the presence of estrogen receptors and the effect is blocked by anti-estrogens. However, there is limited information regarding the regulation of NHERF1 by estrogens in normal colon tissue. The NHERF1 protein has an important role in the maintenance of the intestine ultrastructure. NHERF1-deficient mice showed defects in the intestinal microvilli as well as molecular alterations in brush border membrane proteins. Here, we have studied the expression of NHERF1 in normal rat colon and uterus during the reproductive cycle of Wistar rats. We found that NHERF1 expression in rat colon during the estral cycle is modified by estrogen levels: higher expression of NHERF1 was observed during the proestrous and estrous stages and lower expression in diestrous 1 when estrogen levels decreased. In uterus, NHERF1 was expressed in the apical region of the luminal epithelium and glands in all stages of the estral cycle, and in both colon and uterus, the expression was independent of the proliferation status. Our results show that NHERF1 expression is regulated by estrogens in colon during the rat estral cycle.
authors.affiliation: Institute of Experimental Medicine and Biology of Cuyo (IMBECU), Technology and Scientific Center (CCT), National Research Council of Argentina (CONICET), Mendoza, Argentina.
authors.forename: F Darío
authors.initials: FD
authors.lastname: Cuello-Carrión
authors.forename: Mariana
authors.initials: M
authors.lastname: Troncoso
authors.forename: Elina
authors.initials: E
authors.lastname: Guiñazu
authors.forename: Susana R
authors.initials: SR
authors.lastname: Valdez
authors.forename: Mariel A
authors.initials: MA
authors.lastname: Fanelli
authors.forename: Daniel R
authors.initials: DR
authors.lastname: Ciocca
authors.forename: Erica L
authors.initials: EL
authors.lastname: Kreimann
mesh_terms: D000818:Animals; D003106:Colon / Q000187:drug effects / Q000378:metabolism*; D004031:Diestrus / Q000378:metabolism; D004967:Estrogens / Q000502:physiology*; D030762:Estrous Cycle / Q000502:physiology*; D004971:Estrus / Q000378:metabolism; D005260:Female; D007413:Intestinal Mucosa / Q000378:metabolism; D010750:Phosphoproteins / Q000096:biosynthesis*; D011359:Proestrus / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D017923:Sodium-Hydrogen Exchangers / Q000096:biosynthesis*; D014599:Uterus / Q000187:drug effects / Q000378:metabolism*
chemical_list: D004967:Estrogens; D010750:Phosphoproteins; D017923:Sodium-Hydrogen Exchangers; C093376:sodium-hydrogen exchanger regulatory factor