title: Modifying infrared scattering effects of single yeast cells with plasmonic metal mesh.
abstract: The scattering effects in the infrared (IR) spectra of single, isolated bread yeast cells (Saccharomyces cerevisiae) on a ZnSe substrate and in metal microchannels have been probed by Fourier transform infrared imaging microspectroscopy. Absolute extinction [(3.4±0.6)×10(-7) cm(2) at 3178 cm(-1)], scattering, and absorption cross sections for a single yeast cell and a vibrational absorption spectrum have been determined by comparing it to the scattering properties of single, isolated, latex microspheres (polystyrene, 5.0 μm in diameter) on ZnSe, which are well modeled by the Mie scattering theory. Single yeast cells were then placed into the holes of the IR plasmonic mesh, i.e., metal films with arrays of subwavelength holes, yielding "scatter-free" IR absorption spectra, which have undistorted vibrational lineshapes and a rising generic IR absorption baseline. Absolute extinction, scattering, and absorption spectral profiles were determined for a single, ellipsoidal yeast cell to characterize the interplay of these effects.
authors.affiliation: Department of Chemistry, The Ohio State University, 100 West 18th Avenue, Columbus, Ohio 43210-1173, USA.
authors.forename: Marvin A
authors.initials: MA
authors.lastname: Malone
authors.forename: Suraj
authors.initials: S
authors.lastname: Prakash
authors.forename: Joseph M
authors.initials: JM
authors.lastname: Heer
authors.forename: Lloyd D
authors.initials: LD
authors.lastname: Corwin
authors.forename: Katherine E
authors.initials: KE
authors.lastname: Cilwa
authors.forename: James V
authors.initials: JV
authors.lastname: Coe
mesh_terms: D012441:Saccharomyces cerevisiae / Q000166:cytology*; D018036:Selenium Compounds / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D017967:Zinc Compounds / Q000737:chemistry*
chemical_list: D018036:Selenium Compounds; D017967:Zinc Compounds; C044696:zinc selenide