title: Application and equivalence assessment for determining ethamsylate by using potassium ferricyanide as spectroscopic probe reagent.
abstract: In this paper, a novel method has been established to determine ethamsylate using potassium ferricyanide as a spectroscopic probe reagent. It has been demonstrated that Fe(III) is reduced to Fe(II) by ethamsylate, and that the formed Fe(II) reacts with potassium ferricyanide to form soluble prussian blue (KFe(III)[Fe(II)(CN)(6)]). Beer's law is obeyed in the range of 0.16 - 24.00 µg mL(-1) with the molar absorption coefficient of 2.1 × 10(4) L mol(-1) cm(-1). The detection limit (3 σ/k) is 0.11 µg mL(-1). This method has been successfully applied to determine ethamsylate in pharmaceutical and serum samples with satisfactory results, and presented quite satisfactory credibility during method equivalence assessment.
authors.affiliation: College of Chemistry and Environmental Science, Henan Normal University, Xinxiang, Henan 453007, PR China.
authors.forename: Litao
authors.initials: L
authors.lastname: Liu
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Quanmin
authors.initials: Q
authors.lastname: Li
mesh_terms: D002138:Calibration; D002712:Chlorides / Q000737:chemistry; D004979:Ethamsylate / Q000032:analysis*; D005290:Ferric Compounds / Q000737:chemistry; D005292:Ferricyanides / Q000737:chemistry*; D005295:Ferrocyanides / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010084:Oxidation-Reduction; D013056:Spectrophotometry, Ultraviolet
chemical_list: D002712:Chlorides; D005290:Ferric Compounds; D005292:Ferricyanides; D005295:Ferrocyanides; D004979:Ethamsylate; C000170:ferric ferrocyanide; C024555:ferric chloride; C028033:potassium ferricyanide