title: Induction of new ADAM related proteins from treated human Chang-liver cells.
abstract: Chang-liver cells is a cell line generated from human liver tissue, which is often used in scientific research. ADAMs are a family of proteins that consist of multi-domains, possess multi-functions and play a central role in normal or abnormal physiological conditions, such as regeneration and tumorigenesis. To investigate the expression and functional alteration of the ADAMs or ADAM related proteins in Chang-liver cells, this cell line was treated with heat stress, modified Hanks solution containing ATP or other buffers. Our results showed that the treatment with Hanks solution containing ATP induces Chang-liver cells to express new ADAM related proteins. To analyze these new ADAM related proteins, a cDNA expression library was constructed for the treated Chang-liver cells. A series of positive clones were obtained through immunoscreening with an ADAMs common antibody. A new ADAM related protein possessing alkaline protease activity was confirmed in these clones.
authors.affiliation: Department of Biochemistry and Molecular Biology, Xinxiang Medical University, 453003 Xinxiang, China.
authors.forename: C R
authors.initials: CR
authors.lastname: Wang
authors.forename: D Y
authors.initials: DY
authors.lastname: Hou
authors.forename: H G
authors.initials: HG
authors.lastname: Feng
authors.forename: B S
authors.initials: BS
authors.lastname: Yang
authors.forename: C S
authors.initials: CS
authors.lastname: Xu
authors.forename: J T
authors.initials: JT
authors.lastname: Lin
mesh_terms: D051722:ADAM Proteins / Q000096:biosynthesis*; D000255:Adenosine Triphosphate / Q000494:pharmacology*; D002460:Cell Line; D004790:Enzyme Induction / Q000187:drug effects / Q000502:physiology; D015723:Gene Library; D018869:Heat-Shock Response / Q000187:drug effects* / Q000502:physiology*; D006801:Humans; D008099:Liver / Q000378:metabolism*
chemical_list: D000255:Adenosine Triphosphate; D051722:ADAM Proteins