title: Differential expression of cellular microRNAs in HPV-11 transfected cells. An analysis by three different array platforms and qRT-PCR.
abstract: Human papillomavirus type 11 (HPV-11) infects the genital and the respiratory tract leading to condylomas and respiratory papillomatosis. HPV infections are restricted to epithelial tissue and the progression through the virus lifecycle is tightly coordinated to the differentiation of the host cell. The changes of cellular microRNAs by HPV-11 gene expression were investigated in a cell culture model of HaCaT cells transfected with HPV-11, with the goal of understanding which cellular processes were affected by the virus. Human microRNA profiling was conducted on three different array platform systems and because very few microRNAs (miR-663, -638, -149* and -92b*) were consistently found in all three array data sets we performed extensive statistical analyses of the array data and the qRT-PCR validation. We assume that the most reliable differentially expressed microRNAs are the ones identified by more than one array platform. We also show that TaqMan® qRT-PCR validation is of limited use for less abundant microRNAs.
authors.affiliation: Institute of Cellular and Molecular Medicine, DNA Tumor Virus Laboratory, University of Copenhagen, Panum Institute, Blegdamsvej 3, DK-2200, Denmark.
authors.forename: Anita
authors.initials: A
authors.lastname: Dreher
authors.forename: Maria
authors.initials: M
authors.lastname: Rossing
authors.forename: Bogumil
authors.initials: B
authors.lastname: Kaczkowski
authors.forename: Finn Cilius
authors.initials: FC
authors.lastname: Nielsen
authors.forename: Bodil
authors.initials: B
authors.lastname: Norrild
mesh_terms: D002460:Cell Line; D020869:Gene Expression Profiling; D052140:Human papillomavirus 11 / Q000235:genetics*; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D030361:Papillomavirus Infections / Q000235:genetics* / Q000821:virology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection
chemical_list: D035683:MicroRNAs