title: A dual-emission Förster resonance energy transfer nanoprobe for sensing/imaging pH changes in the biological environment.
abstract: A dual-emission nanoprobe that can sense changes in the environmental pH is designed based on the concept of pH-responsive Förster resonance energy transfer induced by the conformational transition of an associating polyelectrolyte, N-palmitoyl chitosan, bearing a donor (Cy3) or an acceptor (Cy5) moiety. We demonstrate that the developed pH-responsive nanoprobe can be used to ratiometrically image and thus discriminate the pH changes in the biological environment at different length scales.
authors.affiliation: Department of Chemical Engineering, National Tsing Hua University, Hsinchu 30013, Taiwan.
authors.forename: Ya-Ling
authors.initials: YL
authors.lastname: Chiu
authors.forename: Show-An
authors.initials: SA
authors.lastname: Chen
authors.forename: Jean-Hong
authors.initials: JH
authors.lastname: Chen
authors.forename: Ko-Jie
authors.initials: KJ
authors.lastname: Chen
authors.forename: Hsin-Lung
authors.initials: HL
authors.lastname: Chen
authors.forename: Hsing-Wen
authors.initials: HW
authors.lastname: Sung
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D004705:Endocytosis; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D057054:Molecular Imaging / Q000379:methods*; D049329:Nanostructures / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes