title: Release of Cyt c from the model membrane due to conformational change induced by anticancer palladium complex.
abstract: Cytochrome C (Cyt c) is an electron transporting protein that resides within the inter-membrane space of the mitochondria. It plays a critical role as an electron carrier in the process of oxidative phosphorylation and production of cellular ATP. Cyt c is also involved in the apoptosis process and functions as a death messenger. On the other hand, it is well known that the metallo-pharmaceuticals such as palladium complex offer potential as anti-tumor agents to fight cancer. In order to identify the role of anticancer Pd complex in release of Cyt c from the biological membrane, an artificial monolayer was assembled which is able to adsorb Cyt c. A monolayer containing a mixture of two long chain thiols (mercapto-undecanoic acid and mercapto-undecanol) was self-assembled on the surface of a gold electrode. Due to the existence of both hydrophobic and electrostatic interactions between Cyt c and the assembled monolayer, this membrane could be considered as a rough analogue of the biological membrane to study the release of Cyt c by Pd complex. The electrochemical and spectroscopic studies showed that bounding of Pd complex to Cyt c causes a conformational change which leads to the release of Cyt c from the model membrane.
authors.affiliation: Department of Biophysics, Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran.
authors.forename: Sanaz
authors.initials: S
authors.lastname: Emami
authors.forename: Hedayatollah
authors.initials: H
authors.lastname: Ghourchian
authors.forename: Adeleh
authors.initials: A
authors.lastname: Divsalar
mesh_terms: D000327:Adsorption / Q000187:drug effects; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002942:Circular Dichroism; D045304:Cytochromes c / Q000737:chemistry* / Q000378:metabolism*; D055664:Electrochemical Techniques; D004566:Electrodes; D006736:Horses; D055767:Immobilized Proteins / Q000737:chemistry / Q000378:metabolism; D008567:Membranes, Artificial; D010078:Oxazines / Q000378:metabolism; D010165:Palladium / Q000737:chemistry / Q000494:pharmacology*; D017433:Protein Structure, Secondary; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013997:Time Factors
chemical_list: D000970:Antineoplastic Agents; D055767:Immobilized Proteins; D008567:Membranes, Artificial; D010078:Oxazines; D010165:Palladium; D045304:Cytochromes c; C044808:nile red