title: Endothelial cell talin1 is essential for embryonic angiogenesis.
abstract: Using Tln1(fl/fl);CreER mice, we show that tamoxifen-induced inactivation of the talin1 gene throughout the embryo produces an angiogenesis phenotype that is restricted to newly forming blood vessels. The phenotype has a rapid onset in early embryos, resulting in vessel defects by 48 h and death of the embryo within 72 h. Very similar vascular defects were obtained using a Tie2-Cre endothelial cell-specific Tln1 knockout, a phenotype that was rescued by expression of a Tln1 mini-gene in endothelial cells. We show that endothelial cells, unlike most other cell types, do not express talin2, which can compensate for loss of talin1, and demonstrate for the first time that endothelial cells in vivo lacking talin1 are unable to undergo the cell spreading and flattening required to form vessels.
authors.affiliation: Department of Biochemistry, University of Leicester, Lancaster Road, Leicester, LE1 9HN, UK. sjm26@le.ac.uk
authors.forename: Susan J
authors.initials: SJ
authors.lastname: Monkley
authors.forename: Vassiliki
authors.initials: V
authors.lastname: Kostourou
authors.forename: Lorraine
authors.initials: L
authors.lastname: Spence
authors.forename: Brian
authors.initials: B
authors.lastname: Petrich
authors.forename: Stacey
authors.initials: S
authors.lastname: Coleman
authors.forename: Mark H
authors.initials: MH
authors.lastname: Ginsberg
authors.forename: Catrin A
authors.initials: CA
authors.lastname: Pritchard
authors.forename: David R
authors.initials: DR
authors.lastname: Critchley
mesh_terms: D000818:Animals; D015153:Blotting, Western; D017931:DNA Primers / Q000235:genetics; D004622:Embryo, Mammalian; D042783:Endothelial Cells / Q000378:metabolism* / Q000502:physiology; D020868:Gene Silencing / Q000187:drug effects; D006652:Histological Techniques; D007150:Immunohistochemistry; D051379:Mice; D018345:Mice, Knockout; D008854:Microscopy, Electron; D018919:Neovascularization, Physiologic / Q000502:physiology*; D016608:Talin / Q000235:genetics / Q000378:metabolism*; D013629:Tamoxifen
chemical_list: D017931:DNA Primers; D016608:Talin; C496077:Tln1 protein, mouse; D013629:Tamoxifen