title: PIH1D1, a subunit of R2TP complex, inhibits doxorubicin-induced apoptosis.
abstract: We have previously reported that the two components of R2TP complex, RNA polymerase II-associated protein 3 (RPAP3), and Reptin, regulate apoptosis. Here we characterize another component of the complex, PIH1 domain containing protein 1 (PIH1D1). PIH1D1 interacts with both RPAP3 and Monad in HEK293 or U2OS cells. PIH1D1 transcripts were abundant in lung, leukocyte, and placenta. The reduction in endogenous PIH1D1 by siRNA enhanced apoptosis and caspase-3 activation induced by doxorubicin in U2OS cells. These results suggest that PIH1D1 may also function as a novel modulator of apoptosis pathway.
authors.affiliation: Department of Pharmacology, Graduate School of Dentistry, Osaka University, Suita, Osaka, Japan.
authors.forename: Mika
authors.initials: M
authors.lastname: Inoue
authors.forename: Makio
authors.initials: M
authors.lastname: Saeki
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Egusa
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Niwa
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Kamisaki
mesh_terms: D000074183:ATPases Associated with Diverse Cellular Activities; D017209:Apoptosis; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism*; D002460:Cell Line; D045744:Cell Line, Tumor; D004265:DNA Helicases / Q000378:metabolism*; D004317:Doxorubicin / Q000494:pharmacology; D006801:Humans; D014018:Tissue Distribution
chemical_list: D051017:Apoptosis Regulatory Proteins; D002352:Carrier Proteins; C509833:DNAAF10 protein, human; C555143:PIH1D1 protein, human; C472351:RPAP3 protein, human; D004317:Doxorubicin; D000074183:ATPases Associated with Diverse Cellular Activities; D004265:DNA Helicases; C122079:RUVBL2 protein, human