title: [In vivo imaging analysis of biodistribution of FITC-labeled Rituximab in lymphoma-bearing nude mice].
abstract: OBJECTIVE
  To conduct an in vivo optical imaging analysis of the biodistribution of antibody Rituximab in lymphoma tumor-bearing nude mice.
  
  
  METHODS
  Laser scanning confocal microscope and flow cytometry were employed to determine the affinity of FITC-labeled Rituximab (FITC-Rituximab) with human lymphoma Raji cells. And the in vivo optical imaging system was used to analyze the biodistribution of FITC-Rituximab in lymphoma-transplanted xenograft nude mice.
  
  
  RESULTS
  The results of flow cytometry and laser scanning confocal microscope demonstrated that FITC-Rituximab had remarkable affinity with lymphoma Raji cells and was mainly bound at cell membrane. The results of in vivo imaging analysis suggested that FITC-Rituximab could specifically accumulated at peritumor tissue less than 1 h, then penetrated into the interior of tumor and concentrated in 3-4 h. And the specific concentration of FITC-Rituximab could still been observed more than 8-10 h whereas there was no apparent fluorescence at other tissues. Furthermore, the results observed from a two-flank tumor xenograft model showed that FITC-Rituximab possessed specific binding affinity for CD20-overexpressed lymphoma.
  
  
  CONCLUSIONS
  The in vivo optical imaging system can accurately monitor the distribution of FITC-Rituximab in tumor-bearing nude mice. And this technique has a reference value and significance for a real-time analysis of tumor-targeting capability of antibody drugs.
authors.affiliation: Department of Oncology, Chinese Academy of Medical Sciences, Beijing 100050, China.
authors.forename: Sheng-hua
authors.initials: SH
authors.lastname: Zhang
authors.forename: Xin
authors.initials: X
authors.lastname: Cheng
authors.forename: Gen-shen
authors.initials: GS
authors.lastname: Zhong
authors.forename: Dong-sheng
authors.initials: DS
authors.lastname: Xiong
authors.forename: Rong-guang
authors.initials: RG
authors.lastname: Shao
mesh_terms: D000818:Animals; D058846:Antibodies, Monoclonal, Murine-Derived / Q000493:pharmacokinetics*; D045744:Cell Line, Tumor; D005260:Female; D005434:Flow Cytometry; D016650:Fluorescein-5-isothiocyanate / Q000494:pharmacology; D006801:Humans; D016393:Lymphoma, B-Cell / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D018613:Microscopy, Confocal; D000069283:Rituximab; D014018:Tissue Distribution
chemical_list: D058846:Antibodies, Monoclonal, Murine-Derived; D000069283:Rituximab; D016650:Fluorescein-5-isothiocyanate