title: Tetracycline sensing using novel doxycycline derivatives immobilized on different surface plasmon resonance biosensor surfaces.
abstract: BACKGROUND
  This article aims to explore novel doxycycline derivatives for analyzing low concentrations of tetracyclines in biological matrices and food in competitive assays.
  
  
  RESULTS
  Surface plasmon resonance (SPR) was employed in an indirect competitive format using a bacterial tetracycline-dependent regulatory protein as receptor. Three doxycycline derivatives were synthesized and covalently bound to the surface of four different sensor chips. Parameters that influence the immobilization of the doxycycline derivatives and subsequent binding of the receptor protein were studied.
  
  
  CONCLUSIONS
  The novel doxycycline derivatives were successfully used as competitors in an indirect SPR assay.
authors.affiliation: Department of Food Chemistry, Faculty of Mathematics and Natural Sciences, University Wuppertal, Gauss-Str. 20, 42119 Wuppertal, Germany.
authors.forename: Ulrike
authors.initials: U
authors.lastname: Andree
authors.forename: Erika
authors.initials: E
authors.lastname: Mueller-Seitz
authors.forename: Igor
authors.initials: I
authors.lastname: Usai
authors.forename: Stefan
authors.initials: S
authors.lastname: Loeber
authors.forename: Peter
authors.initials: P
authors.lastname: Gmeiner
authors.forename: Cornelius
authors.initials: C
authors.lastname: Wimmer
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Hillen
authors.forename: Michael
authors.initials: M
authors.lastname: Petz
mesh_terms: D000818:Animals; D001667:Binding, Competitive; D004318:Doxycycline / Q000031:analogs & derivatives* / Q000737:chemistry*; D005504:Food Analysis; D006801:Humans; D020349:Surface Plasmon Resonance / Q000379:methods*; D013499:Surface Properties; D013752:Tetracycline / Q000032:analysis* / Q000737:chemistry
chemical_list: D013752:Tetracycline; D004318:Doxycycline