title: Neutralization of BDNF attenuates the in vitro protective effects of olfactory ensheathing cell-conditioned medium on scratch-insulted retinal ganglion cells.
abstract: Transplantation of olfactory ensheathing cells (OECs) becomes one of the promising strategies in restoring lost functions of injured central nervous system. Elevated level of expressed brain-derived neurotrophic factor (BDNF) was revealed in the previous studies to be related to the protective effects of OECs on injured cortical and brain stem neurons as well as retinal ganglion cells (RGCs), but no evidence has been obtained to demonstrate whether transplanted OECs protect injured central neurons directly by their secreted BDNF. In the present study, the effects of BDNF neutralization on the neuroprotection of adult OEC-conditioned medium (OEC-CM) on scratch-insulted RGCs were examined. The results showed that OEC-CM protected cultured RGCs from scratch insult, and neutralization of BDNF by BDNF neutralizing antibody attenuated such neuroprotection of the medium. It is thus concluded that neurotrophic factors including BDNF secreted by OECs can protect injured OECs in vitro and BDNF plays a major role in such a protection of OECs.
authors.affiliation: Institute of Neurosciences, The Fourth Military Medical University, Xi'an, China. waterwang1983@hotmail.com
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.forename: Rui
authors.initials: R
authors.lastname: Cong
authors.forename: Hao
authors.initials: H
authors.lastname: Yang
authors.forename: Ming-Mei
authors.initials: MM
authors.lastname: Wu
authors.forename: Na
authors.initials: N
authors.lastname: Luo
authors.forename: Fang
authors.initials: F
authors.lastname: Kuang
authors.forename: Si-Wei
authors.initials: SW
authors.lastname: You
mesh_terms: D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000037:antagonists & inhibitors* / Q000378:metabolism; D002478:Cells, Cultured; D017077:Culture Media, Conditioned / Q000494:pharmacology*; D008297:Male; D009830:Olfactory Bulb / Q000166:cytology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012165:Retinal Ganglion Cells / Q000187:drug effects* / Q000473:pathology*; D014404:Tubulin / Q000378:metabolism
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D017077:Culture Media, Conditioned; C488416:Tubb3 protein, rat; D014404:Tubulin