title: HPLC analysis of polyphenols in the fruits of Rubus idaeus L. (Rosaceae).
abstract: The separation of anthocyanins present in the fruits of 11 varieties of red raspberries (Rubus idaeus L.) was performed by high performance liquid chromatography (HPLC) with a diode-array detector and evaporative light scattering detection (ELSD). The ELSD parameters--drift tube temperature, nebulising gas flow rate and gain value--were optimised to get the best detection and identification of the anthocyanins. The varieties Heritage and Willamette had the simplest anthocyanin sets consisting of only two predominant anthocyanins--cyanidin-3-O-sophoroside (1) and cyanidin-3-O-glucoside (3), while in the other varieties two other predominant compounds were also present, cyanidin-3-O-rutinoside (4) and cyanidin-3-O-(2(G)-O-glucosylrutinoside) (2). Moreover, using ELSD, simultaneous analysis of anthocyanins and sanguiin H-6 (5), an ellagitannin, was performed. The contents of anthocyanins and sanguiin H-6 (5) were estimated by HPLC with ultraviolet-visible (UV-vis) light detection. The determined concentrations of anthocyanins varied from 76.22 to 277.06 mg per 100 g of dry weight (d.w.). The content of sanguiin H-6 (5) was in the range from 135.04 to 547.48 mg per 100 g of d.w.
authors.affiliation: Department of Pharmacognosy, Medical University of Gdańsk, 80-416 Gdańsk, Poland.
authors.forename: B
authors.initials: B
authors.lastname: Sparzak
authors.forename: M
authors.initials: M
authors.lastname: Merino-Arevalo
authors.forename: Y
authors.initials: Y
authors.lastname: Vander Heyden
authors.forename: M
authors.initials: M
authors.lastname: Krauze-Baranowska
authors.forename: M
authors.initials: M
authors.lastname: Majdan
authors.forename: I
authors.initials: I
authors.lastname: Fecka
authors.forename: D
authors.initials: D
authors.lastname: Głód
authors.forename: T
authors.initials: T
authors.lastname: Bączek
mesh_terms: D000872:Anthocyanins / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D005419:Flavonoids / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry*; D005960:Glucosides / Q000737:chemistry; D015394:Molecular Structure; D010636:Phenols / Q000737:chemistry*; D059808:Polyphenols; D027824:Rosaceae / Q000737:chemistry*
chemical_list: D000872:Anthocyanins; D005419:Flavonoids; D005960:Glucosides; D010636:Phenols; D059808:Polyphenols; C462279:cyanidin-3-O-beta-glucopyranoside; C428983:cyanidin 3-rutinoside