title: Attenuation of food intake in chicks by an inverse agonist of cannabinoid receptor 1 administered by either injection or ingestion in hydrocolloid carriers.
abstract: The cannabinoid receptor (CB(1)) was studied primarily in mammals where it was found to comprise a link between reward processes and addictive behavior such as food consumption. The purpose of this study was twofold: first to characterize the effect of the chicken CB(1) receptor inverse agonist AM251 on food intake, and second, to establish a stress-free approach for application of AM251 to birds using hydrocolloid carriers, which can be mixed with food. A single administration of AM251 by intravenous injection (at 0.85 or 5 mg kg(-1)BW) or by ingestion of hydrocolloid carriers entrapping AM251 at a concentration of 5 mg kg(-1)BW led to a transient attenuation of food intake. The consequent reduced cumulative food intake and BW were observed in the treated chicks for at least 7h post-administration, with no gender differences. Circulating levels of AM251, assessed by LC-MS following 48 h of continuous feeding with hydrocolloid carriers containing 50mg AM 251 kg(-1) BW day(-1), were physiologically significant at 186 ± 73 pmol ml(-1). It is concluded that unlike some other factors, which act differently in birds compared to mammals such as ghrelin, CB(1) inverse agonists attenuate food intake in chicks similar to its effect in mammals. In addition, the new approach for administration of AM251 to birds in hydrocolloid carriers could provide a simple and stress-free tool for prolonged studies of this control mechanism in birds.
authors.affiliation: The Hebrew University of Jerusalem, The Robert H Smith Faculty of Agriculture, Food and Environment, Institute of Biochemistry, Food Science and Nutrition, PO Box 12, Rehovot 76100, Israel.
authors.forename: Nataly
authors.initials: N
authors.lastname: Novoseletsky
authors.forename: Amos
authors.initials: A
authors.lastname: Nussinovitch
authors.forename: Miriam
authors.initials: M
authors.lastname: Friedman-Einat
mesh_terms: D000818:Animals; D002645:Chickens; D003102:Colloids / Q000494:pharmacology; D004337:Drug Carriers / Q000008:administration & dosage; D004435:Eating / Q000187:drug effects; D005260:Female; D008297:Male; D010880:Piperidines / Q000008:administration & dosage / Q000494:pharmacology*; D011720:Pyrazoles / Q000008:administration & dosage / Q000494:pharmacology*; D043884:Receptor, Cannabinoid, CB1 / Q000819:agonists / Q000187:drug effects; D012737:Sex Factors
chemical_list: D003102:Colloids; D004337:Drug Carriers; D010880:Piperidines; D011720:Pyrazoles; D043884:Receptor, Cannabinoid, CB1; C103505:AM 251