title: Determination of Bisphenol A and its chlorinated derivatives in placental tissue samples by liquid chromatography-tandem mass spectrometry.
abstract: The group of compounds commonly called endocrine disruptors covers a wide range of synthetic and natural substances able to alter the normal hormone function of wildlife and humans, consequently causing adverse health effects. Bisphenol A (BPA) and its chlorinated derivatives are some of these compounds. In this work, we propose a new liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to determine these compounds in human placental tissue samples. The method involves an extraction phase of the extracts from the samples using ethyl acetate, followed by a clean-up phase by centrifugation prior to their quantification by LC-MS/MS using an atmospheric pressure chemical ionization (APCI) interface in the negative mode. Deuterated Bisphenol A (BPA-d(16)) was used as internal standard. Found detection limits (DL) ranged from 0.2 to 0.6 ng g(-1) and quantification limits (QL) from 0.5 to 2.0 ng g(-1) for Bisphenol A and its chlorinated derivatives, while inter- and intra-day variability was under 8.1%. The method was validated using standard addition calibration and a spike recovery assay. Recovery rates for spiked samples ranged from 97% to 105%. This method was satisfactorily applied to the determination of BPA and its chlorinated derivatives in 49 placental tissue samples collected from women who live in the province of Granada (Spain).
authors.affiliation: Research Group of Analytical Chemistry and Life Sciences, Department of Analytical Chemistry, University of Granada, Campus of Fuentenueva, E-18071 Granada, Spain.
authors.forename: I
authors.initials: I
authors.lastname: Jiménez-Díaz
authors.forename: A
authors.initials: A
authors.lastname: Zafra-Gómez
authors.forename: O
authors.initials: O
authors.lastname: Ballesteros
authors.forename: N
authors.initials: N
authors.lastname: Navea
authors.forename: A
authors.initials: A
authors.lastname: Navalón
authors.forename: M F
authors.initials: MF
authors.lastname: Fernández
authors.forename: N
authors.initials: N
authors.lastname: Olea
authors.forename: J L
authors.initials: JL
authors.lastname: Vílchez
mesh_terms: D001559:Benzhydryl Compounds; D017606:Chlorine Compounds / Q000032:analysis* / Q000737:chemistry; D002853:Chromatography, Liquid / Q000379:methods*; D052244:Endocrine Disruptors / Q000032:analysis / Q000737:chemistry; D005260:Female; D006801:Humans; D006843:Hydrocarbons, Chlorinated / Q000032:analysis* / Q000737:chemistry; D016014:Linear Models; D010636:Phenols / Q000032:analysis* / Q000737:chemistry; D010920:Placenta / Q000737:chemistry*; D011247:Pregnancy; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D017606:Chlorine Compounds; D052244:Endocrine Disruptors; D006843:Hydrocarbons, Chlorinated; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds