title: Effect of icariin on bone formation during distraction osteogenesis in the rabbit mandible.
abstract: The aim of this study was to evaluate the effect of icariin on bone formation during mandibular distraction. 40 Rabbits were randomly divided into experimental and control groups. Mandibular distraction was performed 5 days after unilateral mandibular osteotomy using a custom-made external distractor at a rate of 0.5mm/12h for 10 days. From the first day of distraction, icariin (2.5mg/kg · day) was orally administered to the experimental group and placebo to the controls. 10 Rabbits were killed at the end of weeks 2 and 4 of the consolidation phase. The distracted hemimandible was harvested and newly formed bone was evaluated by soft radiography, histology and bone histomorphometry. Regenerated bone was evaluated for bone mineral density by dual-energy X-ray absorptiometry. The experimental group had fewer radiolucent areas on soft radiography. Bone mineral density of regenerated bone was higher in the experimental than in the control group at 2 and 4 weeks. At 4 weeks, the experimental group had greater volumes of new bone, higher trabecular number, and less trabecular separation than the controls. Oral administration of icariin could promote bone formation during mandibular distraction osteogenesis and might be a promising method for shortening the course of distraction osteogenesis.
authors.affiliation: Center of Orthognathic Surgery, Peking University School of Stomatology, Beijing, People's Republic of China.
authors.forename: H
authors.initials: H
authors.lastname: Wei
authors.forename: L
authors.initials: L
authors.lastname: Zili
authors.forename: C
authors.initials: C
authors.lastname: Yuanlu
authors.forename: Y
authors.initials: Y
authors.lastname: Biao
authors.forename: L
authors.initials: L
authors.lastname: Cheng
authors.forename: W
authors.initials: W
authors.lastname: Xiaoxia
authors.forename: L
authors.initials: L
authors.lastname: Yang
authors.forename: W
authors.initials: W
authors.lastname: Xing
mesh_terms: D015502:Absorptiometry, Photon; D000284:Administration, Oral; D000818:Animals; D015519:Bone Density / Q000187:drug effects; D001861:Bone Regeneration / Q000187:drug effects*; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D005419:Flavonoids / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D008297:Male; D008334:Mandible / Q000000981:diagnostic imaging / Q000601:surgery*; D015394:Molecular Structure; D019857:Osteogenesis, Distraction / Q000379:methods; D011817:Rabbits; D011897:Random Allocation
chemical_list: D004365:Drugs, Chinese Herbal; D005419:Flavonoids; C056599:icariin