title: Reorganization and caging of DPPC, DPPE, DPPG, and DPPS monolayers caused by dimethylsulfoxide observed using Brewster angle microscopy.
abstract: The interaction between dimethylsulfoxide (DMSO) and phospholipid monolayers with different polar headgroups was studied using "in situ" Brewster angle microscopy (BAM) coupled to a Langmuir trough. For a 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) monolayer, DMSO was shown to significantly impact the structure of the liquid expanded (LE) and gaseous phases. The domains reorganized to much larger domain structures. Domains in the liquid condensed (LC) phase were formed on the DMSO-containing subphase at the mean molecular area where only gaseous and LE phases were previously observed on the pure water subphase. These results clearly demonstrate the condensing and caging effect of DMSO molecules on the DPPC monolayer. Similar effects were found on dipalmitoyl phosphatidyl ethanolamine, glycerol, and serine phospholipids, indicating that the condensing and caging effect is not dependent upon the phospholipid headgroup structure. The DMSO-induced condensing and caging effect is the molecular mechanism that may account for the enhanced permeability of membranes upon exposure to DMSO.
authors.affiliation: Department of Chemistry, The Ohio State University, 100 West 18th Avenue, Columbus, Ohio 43210, United States.
authors.forename: Xiangke
authors.initials: X
authors.lastname: Chen
authors.forename: Zishuai
authors.initials: Z
authors.lastname: Huang
authors.forename: Wei
authors.initials: W
authors.lastname: Hua
authors.forename: Hardy
authors.initials: H
authors.lastname: Castada
authors.forename: Heather C
authors.initials: HC
authors.lastname: Allen
mesh_terms: D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry; D002462:Cell Membrane / Q000737:chemistry; D004121:Dimethyl Sulfoxide / Q000737:chemistry*; D020404:Glycerophospholipids / Q000737:chemistry*; D008853:Microscopy; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010715:Phosphatidylglycerols / Q000737:chemistry; D010718:Phosphatidylserines / Q000737:chemistry
chemical_list: D020404:Glycerophospholipids; D010714:Phosphatidylethanolamines; D010715:Phosphatidylglycerols; D010718:Phosphatidylserines; D015060:1,2-Dipalmitoylphosphatidylcholine; C007510:1,2-dipalmitoyl-3-phosphatidylethanolamine; C038694:dipalmitoylphosphatidylserine; C030345:1,2-dipalmitoylphosphatidylglycerol; D004121:Dimethyl Sulfoxide