title: Increased expression of TNF receptor-associated factor 6 after rat traumatic brain injury.
abstract: TRAF6 (TNF receptor-associated factor 6), a member of tumor necrosis factor receptor-associated factors family was identified as a molecule that binds to the cytoplasmic domain of CD40. TRAF6 functions as an adaptor, positively regulating the NF-κB, JNK pathway. Additionally, some studies have reported that TRAF6 is required for apoptosis within the developing CNS and regulates cell fate decisions by inducing caspase 8-dependent apoptosis. However, its distribution and function in the central nervous system (CNS) lesion are not well understood. In this study, we performed an acute traumatic brain injury model in adult rats. And we mainly examined protein expression and cellular localization of TRAF6 during rat traumatic brain injury (TBI). Western blot analysis showed TRAF6 level significantly improved at 7 days after injury, and then declined during the following days. The protein expression of TRAF6 was further analysed by immunohistochemistry. In comparison to contralateral cerebral cortex, we observed a highly significant accumulation of TRAF6 at the ipsilateral brain. Immunofluorescence double-labeling showed that TRAF6 was co-expressed with NeuN and GFAP. Besides, co-localization of TRAF6/active caspase 3 and TRAF6/proliferating cell nuclear antigen (PCNA) were detected in NeuN and GFAP, respectively. We also examined the expression profiles of proliferating cell nuclear antigen (PCNA) and active caspase 3 whose changes were correlated with the expression profiles of TRAF6. In conclusion, this is the first description of TRAF6 expression in traumatic brains. Our data suggested that TRAF6 might play important roles in CNS pathophysiology after TBI.
authors.affiliation: Department of Neurosurgery, Affiliated Hospital of Nantong University, The Jiangsu Province Key Laboratory of Neuroregeneration, Nantong University, Nantong, People's Republic of China. chenjian9079@163.com
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
authors.forename: Xiujie
authors.initials: X
authors.lastname: Wu
authors.forename: Bai
authors.initials: B
authors.lastname: Shao
authors.forename: Wei
authors.initials: W
authors.lastname: Zhao
authors.forename: Wei
authors.initials: W
authors.lastname: Shi
authors.forename: Shuangwei
authors.initials: S
authors.lastname: Zhang
authors.forename: Lanchun
authors.initials: L
authors.lastname: Ni
authors.forename: Aiguo
authors.initials: A
authors.lastname: Shen
mesh_terms: D000818:Animals; D017209:Apoptosis; D015415:Biomarkers / Q000378:metabolism; D015153:Blotting, Western; D001930:Brain Injuries / Q000378:metabolism* / Q000473:pathology; D049109:Cell Proliferation; D002540:Cerebral Cortex / Q000378:metabolism / Q000473:pathology; D005455:Fluorescent Antibody Technique; D015151:Immunoblotting; D008297:Male; D009474:Neurons / Q000378:metabolism / Q000473:pathology; D021381:Protein Transport; D051381:Rats; D017207:Rats, Sprague-Dawley; D048029:TNF Receptor-Associated Factor 6 / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D048029:TNF Receptor-Associated Factor 6