title: Up-regulation of the isoenzymes MAO-A and MAO-B in the human basal ganglia and pons in Huntington's disease revealed by quantitative enzyme radioautography.
abstract: Huntington's disease (HD) is a rare genetic disease associated with the degeneration of GABAergic striatal projection neurons in the basal ganglia leading to movement disorders with behavioral symptoms for which there is presently no therapy. Abnormally high levels of monoamine oxidase (MAO) activity, which are potentially linked to cytotoxic free radical formation, are known to occur during aging and in neurodegenerative disorders (MAO-B is markedly increased in plaque-associated astrocytes in Alzheimer's disease). We therefore measured, with anatomical resolution, MAO-A and -B activities in 5 cases of HD (severity grades 1-3) and age-matched controls by quantitative enzyme radioautography using radiolabeled enzyme inhibitors (3)H-Ro 41-1049 and (3)H-lazabemide, respectively, as high-affinity ligands in vitro. MAO-A was increased significantly (ca. 50%; p<0.01) in the putamen and substantia nigra pars compacta of the basal ganglia and in the pons. Higher increases in MAO-B (75%-200%; p<0.01) occurred in the putamen, ventral striatum, globus pallidus externus and internus of the basal ganglia and in the insular cortex. The increased enzyme levels (especially of MAO-B) seemed to correlate with the grade of disease severity. We conclude that MAO increases in those regions of HD brains which are known to undergo neurodegeneration accompanied by glioses. Whether or not this increased enzyme activity is a cause or effect of the resulting loss of the GABAergic projection neurons in HD is yet to be clarified. Moreover, it remains to be seen if selective enzyme inhibitors have therapeutic utility in the treatment of HD by reducing oxidative stress locally.
authors.affiliation: Preclinical Neuroscience Research, F Hoffmann-La Roche Ltd, CH-4070 Basel, Switzerland. richards@balcab.ch
authors.forename: Grayson
authors.initials: G
authors.lastname: Richards
authors.forename: Juerg
authors.initials: J
authors.lastname: Messer
authors.forename: Henry J
authors.initials: HJ
authors.lastname: Waldvogel
authors.forename: Hannah M
authors.initials: HM
authors.lastname: Gibbons
authors.forename: Mike
authors.initials: M
authors.lastname: Dragunow
authors.forename: Richard L M
authors.initials: RL
authors.lastname: Faull
authors.forename: Josep
authors.initials: J
authors.lastname: Saura
mesh_terms: D000328:Adult; D000368:Aged; D001479:Basal Ganglia / Q000000981:diagnostic imaging / Q000201:enzymology*; D015415:Biomarkers / Q000378:metabolism; D005260:Female; D006801:Humans; D006816:Huntington Disease / Q000000981:diagnostic imaging / Q000201:enzymology*; D007527:Isoenzymes / Q000096:biosynthesis; D008297:Male; D008875:Middle Aged; D008995:Monoamine Oxidase / Q000096:biosynthesis*; D018384:Oxidative Stress / Q000502:physiology*; D011149:Pons / Q000000981:diagnostic imaging / Q000201:enzymology* / Q000503:physiopathology; D011877:Radionuclide Imaging; D015854:Up-Regulation / Q000502:physiology*
chemical_list: D015415:Biomarkers; D007527:Isoenzymes; D008995:Monoamine Oxidase