Augmentation of regulatory B cell activity in experimental allergic encephalomyelitis by glatiramer acetate.
We recently showed that B cells reduce CNS inflammation in mice with experimental allergic encephalomyelitis (EAE). Here, we demonstrate that adoptively transferred CD5/CD19+ B cells protect against EAE severity. Furthermore, we show that glatiramer acetate (GA), a therapeutic for relapsing multiple sclerosis treatment, amplifies this effect. Transfer of GA-conditioned B cells leads to increased production of immunoregulatory cytokines and reduced CNS inflammation, as well as decreased expression of the chemokine receptor, CXCR5, and elevated BDNF expression in the CNS. Thus B cells can protect against EAE, and GA augments this effect in maintaining immune homeostasis and controlling EAE disease progression.
Department of Medicine, Dartmouth Medical School, Lebanon, NH 03756; Multiple Sclerosis Center@ Dartmouth College, Hanover, NH 03755, USA. Sakhina.Begum-Haque@Dartmouth.edu
Sakhina
S
Begum-Haque
Marc
M
Christy
Javier
J
Ochoa-Reparaz
Elizabeth C
EC
Nowak
Daniel
D
Mielcarz
Azizul
A
Haque
Lloyd H
LH
Kasper
D019264:Adoptive Transfer; D000818:Animals; D018941:Antigens, CD19 / Q000276:immunology / Q000378:metabolism; D016175:B-Lymphocyte Subsets / Q000187:drug effects* / Q000276:immunology* / Q000378:metabolism; D019208:Brain-Derived Neurotrophic Factor / Q000096:biosynthesis / Q000187:drug effects; D018956:CD5 Antigens / Q000276:immunology / Q000378:metabolism; D002469:Cell Separation; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D005260:Female; D005434:Flow Cytometry; D000068717:Glatiramer Acetate; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D051379:Mice; D008810:Mice, Inbred C57BL; D010455:Peptides / Q000494:pharmacology*; D054380:Receptors, CXCR5 / Q000096:biosynthesis / Q000187:drug effects; D020133:Reverse Transcriptase Polymerase Chain Reaction
D018941:Antigens, CD19; D019208:Brain-Derived Neurotrophic Factor; D018956:CD5 Antigens; C513179:CXCR5 protein, mouse; D007166:Immunosuppressive Agents; D010455:Peptides; D054380:Receptors, CXCR5; D000068717:Glatiramer Acetate