Leptin facilitates lordosis behavior through GnRH-1 and progestin receptors in estrogen-primed rats.
Dose response curves for leptin facilitation of estrous behavior (lordosis and proceptivity) were made by infusing the peptide into the lateral ventricle (icv) of ovariectomized (ovx), ad libitum-fed rats injected 40h previously with 5μg of estradiol benzoate. Leptin doses of 1 and 3μg produced significant lordosis quotient at 60min post-injection, with maximal lordosis being displayed at 120min. Yet the intensity of lordosis was weak, and a high incidence of rejection behaviors was found. Moreover, leptin did not induce significant proceptive behaviors at any dose. The leptin doses of 1 and 3μg were selected for determining whether antide, a GnRH-1 receptor antagonist, or the progestin receptor antagonist RU486 could modify the lordosis response to leptin. Icv injection of either antide or RU486 1h before leptin significantly depressed leptin facilitation of lordosis. The results suggest that leptin stimulates lordosis by releasing GnRH, which in turn activates GnRH-1 and progestin receptors. The physiological role of leptin in the control of estrous behavior remains to be determined.
Centro de Investigación en Reproducción Animal, Universidad Autónoma de Tlaxcala-CINVESTAV, Apdo. 62, Tlaxcala, Mexico.
Marcos
M
García-Juárez
Carlos
C
Beyer
Alfonso
A
Soto-Sánchez
Raymundo
R
Domínguez-Ordoñez
Porfirio
P
Gómora-Arrati
Francisco Javier
FJ
Lima-Hernández
José R
JR
Eguibar
Anne M
AM
Etgen
Oscar
O
González-Flores
D000818:Animals; D004967:Estrogens / Q000494:pharmacology*; D005260:Female; D006727:Hormone Antagonists / Q000494:pharmacology; D057967:Infusions, Intraventricular; D020738:Leptin / Q000494:pharmacology*; D008141:Lordosis; D008297:Male; D015735:Mifepristone / Q000494:pharmacology; D009842:Oligopeptides / Q000494:pharmacology; D010052:Ovariectomy; D051381:Rats; D017207:Rats, Sprague-Dawley; D011966:Receptors, LHRH / Q000037:antagonists & inhibitors*; D011980:Receptors, Progesterone / Q000378:metabolism*; D012726:Sexual Behavior, Animal / Q000187:drug effects*
D004967:Estrogens; D006727:Hormone Antagonists; D020738:Leptin; D009842:Oligopeptides; D011966:Receptors, LHRH; D011980:Receptors, Progesterone; D015735:Mifepristone; C057022:iturelix