Human urine biomarkers of renal cell carcinoma evaluated by ClinProt.
Renal cell carcinoma (RCC) is one of the major causes of cancer death and is radio- and chemoresistant. Urine of 29 healthy subjects and 39 clear cell RCC patients were analyzed using the ClinProt technique to search for possible biomarkers for early RCC diagnosis. A cluster of three signals (marker A= at m/z 1827 ± 8 Da, marker B = 1914 ± 8 Da and marker C = 1968 ± 8 Da) was able to discriminate patients from controls. A receiver operating characteristic curve analysis showed values of area under the curve (AUC) higher than 0.9 for marker A and B, corresponding to a sensitivity of 85-90% and a specificity of 90%, while marker C gave a lower AUC (0.84) corresponding to sensitivity of 70% and specificity of 100%. The combination of three markers lead to an improvement in diagnostic efficacy, with specificity and sensitivity of 100% and 95%, respectively, in the training test and of 100% and of 85% in the test experiment. The efficacy of this cluster of signals to distinguish RCC patients grouped by tumor stage showed a sensibility of 100% for patients at the primary tumor 1 stage. One of the signals present in the cluster was identified as a fragment of Tamm-Horsfall protein.
Department of Experimental Medicine, University of Milano-Bicocca, Monza, Italy.
Niccolò
N
Bosso
Clizia
C
Chinello
Stefano Carlo Maria
SC
Picozzi
Erica
E
Gianazza
Veronica
V
Mainini
Carmen
C
Galbusera
Francesca
F
Raimondo
Roberto
R
Perego
Stefano
S
Casellato
Francesco
F
Rocco
Stefano
S
Ferrero
Silvano
S
Bosari
Paolo
P
Mocarelli
Marzia Galli
MG
Kienle
Fulvio
F
Magni