[Study and preparation of a novel apatite-wollastonite bioactive glass-ceramic-calcium sulphate hemihydrate composite].
OBJECTIVE
To prepare a novel apatite-wollastonite bioactive glass-ceramic-calcium sulphate hemihydrate(AW-BGC-CSH) composite, to study its biocompatibility, and to provide experimental support for its further clinical application.


METHODS
Samples of AW-BGC-CSH composite were prepared with different AW-BGC granules-CSH ratios (50%, 40%, 30%, 20%). Surface morphology, microstructure and mechanical features of the composite were measured. Osteoblasts were cultivated in vitro on the composite. Cell morphology, proliferation, and the alkaline phosphatase (ALP) activity of osteoblasts were examined to determine the biocompatibility of the composite.


RESULTS
The composite showed a three-dimensional pored structure with communicated micropores under scanning electron microscopy (SEM). The plasticity of the composite could be maintained within 3 - 5 min. Its top solidification temperature was 36.4°C and the maximum compressive strength was 9.3 MPa. The osteoblasts adhered to the composite and grew well. At 1, 3, 5, 7 d after cultivated, the microprotein contents of the composite were (251 ± 12), (296 ± 31), (580 ± 13) and (571 ± 15) mg/L, and the ALP activity of the composite were (4.50 ± 0.68), (6.90 ± 0.27), (12.05 ± 0.28) and (11.86 ± 0.63) U/mg. The results of the ALP activity and microprotein contents in the experiment group were significantly higher than those in the control group (P < 0.05).


CONCLUSIONS
The prepared AW-BGC-CSH composite has a three-dimensional pored structure, favourable plasticity, mechanical property and good biocompatibility.
Department of Prosthodontics, Sichuan University, Chengdu, China.
Xin-xin
XX
Chen
Yun-mao
YM
Liao
Zhi-min
ZM
Zhu
D001031:Apatites / Q000737:chemistry*; D001672:Biocompatible Materials / Q000737:chemistry*; D017610:Calcium Compounds / Q000737:chemistry*; D002133:Calcium Sulfate / Q000737:chemistry*; D002478:Cells, Cultured; D002516:Ceramics / Q000737:chemistry*; D008422:Materials Testing; D010006:Osteoblasts / Q000166:cytology; D017640:Silicates / Q000737:chemistry*
D001031:Apatites; D001672:Biocompatible Materials; D017610:Calcium Compounds; D017640:Silicates; C031293:calcium silicate; D002133:Calcium Sulfate