Multiplex bead analysis of urinary cytokines of type 2 diabetic patients with normo- and microalbuminuria.
Renal damage of diabetic patients is linked to chronic low-grade inflammation. The aim of this study was to analyze urinary concentrations of 27 cytokines in type 2 diabetic patients with normo- and microalbuminuria by multiplex bead immunoassay. Urinary levels of IL-8, IP-10, MCP-1, G-CSF, EOTAXIN, RANTES, and TNF-α in microalbuminuric patients were significantly increased compared to those in normoalbuminuric patients (p < 0.05) or controls (p < 0.05). GM-CSF, MIP-1α, and MIP-1β levels were more elevated in microalbuminuric patients than in controls (both p < 0.05). IP-10 and MCP-1 levels were correlated with urinary albumin excretion rate (r = 0.668 and 0.544, both p < 0.01), and estimated glomerular filtration rate (r = -0.454 and -0.418, both p < 0.05). EOTAXIN, GM-CSF, IP-10, MCP-1, and RANTES levels were correlated with HbA1c (r = 0.457, 0.466, 0.678, 0.567, 0.542, respectively, p ≤ 0.01). These results indicate that urinary cytokine determination might be useful for the early diagnosis and management of patients with diabetic nephropathy.
Department of Endocrinology, First Hospital Affiliated, Medical School of Xi'an Jiaotong University, Shaanxi, PR China.
Jingfang
J
Liu
Zhenjun
Z
Zhao
Mark D P
MD
Willcox
Banglao
B
Xu
Bingyin
B
Shi
D000419:Albuminuria / Q000652:urine*; D016022:Case-Control Studies; D016207:Cytokines / Q000652:urine*; D003924:Diabetes Mellitus, Type 2 / Q000652:urine*; D003928:Diabetic Nephropathies / Q000175:diagnosis* / Q000652:urine; D019937:Diagnostic Techniques and Procedures; D042241:Early Diagnosis; D006801:Humans; D007118:Immunoassay / Q000379:methods*
D016207:Cytokines