The role of Fas ligand protein in the oxidative stress induced by azoxymethane on crypt colon of rats.
OBJECTIVE
To study the protein Fas ligand (FasL) on the expression of apoptosis, using a model of oxidative stress induced by azoxymethane (AOM), in the crypt of colon in rats.


METHODS
Wistar rats (n=14) were assigned into two groups: control (n=7) and AOM (n=7). A single subcutaneous administration of AOM (5mg/kg) or saline solution was performed at the beginning of third week and after three hours samples of proximal colon were collected. The expression of FasL was quantified (Software ImageLab) in percentage of areas in the top, base and all crypt. Results were expressed as mean ± sd (Shapiro-Wilks test and t Student test) (p < 0.05).


RESULTS
In the animals of CG there was no significant difference between the FasL expression of the top (10.75±3.33) and basal (11.14±3.53) colon crypt (p=0.34293740). In the animals of AOM there was no significant difference between the FasL expression of the top (8.86±4.19) and basal (8.99±4.08) colon crypt (p=0.78486003). In the animals of CG (10.95±3.43) and AOM (8.92±4.13) there was a significant difference of the FasL expression (p=0.026466821). A significantly decrease on the FasL expression was observed in the animals of CG (10.75±3.33) and AOM (8.86±4.19) in the top crypt (p=0.00003755*). A significant decrease was also observed in the animals of CG (11.14±3.53) and AOM (8.99±4.08) in the basal colon crypt (p=0.00000381**).


CONCLUSIONS
Azoxymethane induce the oxidative stress and the significantly decrease of FasL expression, although there is no significant difference between basal and top of colon crypt linked to consumption-activation of Fas ligand.
Department of Surgery, UNIFESP, SP, Brazil.
Luiz Antonio Maksoud
LA
Bussuan
Djalma José
DJ
Fagundes
Guido
G
Marks
Priscila Maksoud
PM
Bussuan
Roberto
R
Teruya
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001397:Azoxymethane; D015415:Biomarkers / Q000378:metabolism; D002273:Carcinogens; D003106:Colon / Q000378:metabolism / Q000473:pathology*; D053222:Fas Ligand Protein / Q000378:metabolism*; D008297:Male; D023421:Models, Animal; D018384:Oxidative Stress / Q000187:drug effects*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
D015415:Biomarkers; D002273:Carcinogens; D053222:Fas Ligand Protein; D001397:Azoxymethane