Citreicella aestuarii sp. nov., isolated from a tidal flat.
A Gram-negative bacterium, designated AD8(T), was isolated from a tidal flat of the Taean coast in South Korea. Cells were strictly aerobic, motile, ovoid rods or rods, with two lateral flagella on one side. Strain AD8(T) was catalase- and oxidase-positive and able to oxidize thiosulfate and reduce nitrate to nitrite. Growth of strain AD8(T) was observed at 15-40 °C (optimum 25-30 °C), at pH 5.0-9.5 (optimum pH 6.0-7.5) and with 1.0-11.0 % (w/v) NaCl (optimum 2.0-4.0 %). The predominant fatty acids were C(18 : 1)ω7c and C(19 : 0) cyclo ω8c. The G+C content of the genomic DNA was 67.5 mol% and the major quinone was Q-10. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain AD8(T) belonged to the genus Citreicella and was most closely related to Citreicella marina CK-I3-6(T) within the class Alphaproteobacteria (96.9 % 16S rRNA gene sequence similarity). On the basis of phenotypic, chemotaxonomic and phylogenetic analysis, strain AD8(T) represents a novel species within the genus Citreicella, for which the name Citreicella aestuarii sp. nov. is proposed. The type strain is AD8(T) ( = KACC 13699(T) = DSM 22011(T)).
Department of Life Science & Research Center for Biomolecules and Biosystems, Chung-Ang University, Seoul, 156-756, Republic of Korea.
Moon Su
MS
Park
Department of Life Science & Research Center for Biomolecules and Biosystems, Chung-Ang University, Seoul, 156-756, Republic of Korea.
Bok-Sil
BS
Chung
Department of Life Science & Research Center for Biomolecules and Biosystems, Chung-Ang University, Seoul, 156-756, Republic of Korea.
Hyo Jung
HJ
Lee
Department of Life Science & Research Center for Biomolecules and Biosystems, Chung-Ang University, Seoul, 156-756, Republic of Korea.
Hyun Mi
HM
Jin
Department of Animal Science & Technology, Sunchon National University, Sunchon 540-742, Republic of Korea.
Sang-Suk
SS
Lee
Animal Nutrition & Physiology Division, National Institute of Animal Science, RDA, Suwon, 441-706, Republic of Korea.
Young Kyoon
YK
Oh
Department of Life Science & Research Center for Biomolecules and Biosystems, Chung-Ang University, Seoul, 156-756, Republic of Korea.
Che Ok
CO
Jeon
D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D041902:Rhodobacteraceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D012623:Seawater / Q000382:microbiology*
D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S