[Characterization of reaction kinetics between ligand and receptor on the solid and liquid interface based on optical waveguide lightmode spectroscopy].
The present paper describes the use of optical waveguide lightmode spectroscopy (OWLS) for study of the binding interaction of the vascular endothelial growth factor (VGEF) with VEGF receptor 2 (VEGFR2). VEGF were immobilized in the surface of an 3-amino 3-propyltriethoxy silane (APTES) modified sensor chip. The solutions with different concentration of VEGFR2 were injected to the system to investigate the kinetic character with OWLS on the solid and liquid interface. The receptor binding and dissociation on the interface, quantified by association and dissociation rate coefficients ka and kd, were determined by the OWLS experiments. The k(a) and k(d) is 6.86 x 10(5) L x mol(-1) x s(-1) and 1.15 x 10(-3) s(-1), respectively. The results show that OWLS method could meet the requirement of kinetic determination of ligand--receptor interaction in applications for related fundamental research and pharmaceutical development.
State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, China. wuzw2001@163.com
Zhong-wei
ZW
Wu
Quan-jun
QJ
Liu
Ling-wei
LW
Wu
Xiao
X
Xie
Zu-hong
ZH
Lu
D007700:Kinetics; D008024:Ligands; D011485:Protein Binding; D013057:Spectrum Analysis; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000378:metabolism*
D008024:Ligands; D042461:Vascular Endothelial Growth Factor A; D040301:Vascular Endothelial Growth Factor Receptor-2