Modulation of oxidative stress by functionalized fullerene materials in the lung tissues of female C57/BL mice with a metastatic Lewis lung carcinoma.
Oxidative stress is considered to be one of the important mechanisms involved in carcinogenesis. To investigate the effect of [Gd@C82(OH)22]n and [C60(OH)20]n nanoparticles on the oxidative stress in the tumor-bearing mice, several antioxidative enzymes and antioxidants were tested for mice with or without tumor inoculation. Transplanted tumors were grown in mice by subcutaneous inoculation of a metastatic Lewis lung carcinoma in female C57/BL mice. More importantly, the tumor cells can metastasize into the normal lung tissues gradually. Therefore, in present paper, the activities of copper-zinc superoxide dismutase (CuZn-SOD), glutathione peroxidase (GSH-Px), glutathione S-transferase (GST), as well as the levels of reduced glutathione (GSH) and malondialdehyde (MDA) in the tumor-invaded lung tissues of the tumor-bearing mice were compared to the nomal lung tissues of normal mice. After treatment with nanoparticles, the activities of GSH-Px and GST and other parameters related to the oxidative stress were downregulated and tended closely to the normal levels. Pulmonary histopathological results also showed that two different types of water-soluble fullerenes can prevent lungs from inflammatory lesion and tumor invasion. These findings indicate two different types of water-soluble fullerenes materials can downregulate the oxidative stress status by scavenging excessive free radicals and inhibiting the lipid peroxidation in tumor-bearing mice, which can partly explain their protective roles on the pulmonary oxidative-damage induced by the tumor metastasis to lung tissues.
CAS Key Lab for Biological Effects of Nanomaterials and Nanosafety (NCNST-IHEP), Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, China.
Fang
F
Jiao
Ying
Y
Qu
Guoqiang
G
Zhou
Ying
Y
Liu
Wei
W
Li
Cuicui
C
Ge
Yufeng
Y
Li
Wei
W
Hu
Bai
B
Li
Yuxi
Y
Gao
Chunying
C
Chen
D000818:Animals; D018827:Carcinoma, Lewis Lung / Q000378:metabolism*; D005260:Female; D037741:Fullerenes / Q000494:pharmacology*; D005682:Gadolinium / Q000494:pharmacology*; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D008168:Lung / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D053768:Metal Nanoparticles / Q000737:chemistry*; D051379:Mice; D008810:Mice, Inbred C57BL; D009362:Neoplasm Metastasis; D009368:Neoplasm Transplantation; D009942:Organometallic Compounds / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D013482:Superoxide Dismutase / Q000378:metabolism
D037741:Fullerenes; D009942:Organometallic Compounds; D005682:Gadolinium; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005982:Glutathione Transferase; D005978:Glutathione