Characterization of a RacGTPase up-regulated in the large yellow croaker Pseudosciaena crocea immunity.
The Rac proteins are members of the Rho family of small G proteins and are implicated in the regulation of several pathways, including those leading to cytoskeleton reorganization, gene expression, cell proliferation, cell adhesion and cell migration and survival. In this investigation, a Rac gene (named as LycRac gene) was obtained from the large yellow croaker and it was expressed in Escherichia coli and purified. Subsequently the specific antibody was raised using the purified fusion protein (GST-LycRac). Moreover, the GTP-binding assay showed that the LycRac protein had GTP-binding activity. The LycRac gene was ubiquitously transcribed and expressed in 9 tissues. Quantitative real-time RT-PCR and Western blot analysis revealed the highest expression in gill and the weakest expression in spleen. Time-course analysis revealed that LycRac expression was obviously up-regulated in blood, spleen and liver after immunization with polyinosinic polycytidynic acid (poly I:C), formalin-inactive Gram-negative bacterium Vibrio parahemolyticus and bacterial lipopolysaccharides (LPS). These results suggested that LycRac protein might play an important role in the immune response against microorganisms in large yellow croaker.
Key Laboratory of Science and Technology for Aquaculture and Food Safety, Fisheries College, Jimei University, Xiamen 361021, China.
Fang
F
Han
Xiaoqing
X
Wang
Qilian
Q
Yang
Mingyi
M
Cai
Zhi Yong
ZY
Wang
D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D008070:Lipopolysaccharides / Q000494:pharmacology; D008969:Molecular Sequence Data; D010473:Perciformes / Q000145:classification / Q000276:immunology* / Q000382:microbiology; D010802:Phylogeny; D011070:Poly I-C / Q000494:pharmacology; D011485:Protein Binding; D016415:Sequence Alignment; D015854:Up-Regulation / Q000187:drug effects / Q000276:immunology; D014736:Vibrio parahaemolyticus / Q000276:immunology; D020744:rac GTP-Binding Proteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology*
D000276:Adjuvants, Immunologic; D008070:Lipopolysaccharides; D020744:rac GTP-Binding Proteins; D011070:Poly I-C