Effects of low concentration BMP-7 on human osteoarthritic chondrocytes: comparison of different applications.
While BMP-7 (OP-1) is one of the most potent growth factors in cartilage tissue engineering, the effects of exogenous low concentration BMP-7 on osteoarthritic chondrocytes are still unknown. Human osteoarthritic chondrocytes obtained from the femoral condyles of 10 patients were grown either in monolayer or in 3D collagen type-I gel culture in vitro. The growth factor was either given as a single dose of 50 ng/mL, a repeated dose, or continuously released from PGLA microspheres. Matrix formation was monitored by immunohistochemical staining and real-time PCR. In contrast to monolayer culture, the differentiated phenotype was prevailed in 3D culture. Collagen type-II protein production in the 3D group with a continuous BMP-7 release was enhanced in comparison to all other groups. Gene expression of collagen type-II and aggrecan was elevated in all treatment groups, with the highest extent in the BMP-7 microsphere group. In summary, treatment of articular chondrocytes with a low dose of BMP-7 leads to an elevated production of extracellular matrix components. This effect is further increased when BMP-7 is given repeatedly or continuously, which proved to be the most effective form of application.
Department of Orthopaedic Surgery, Aachen University Hospital, Aachen, Germany. kgavenis@ukaachen.de
Karsten
K
Gavenis
Nicole
N
Heussen
Bernhard
B
Schmidt-Rohlfing
D053674:Aggrecans / Q000235:genetics; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry; D055419:Bone Morphogenetic Protein 7 / Q000008:administration & dosage* / Q000494:pharmacology*; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D024042:Collagen Type I / Q000737:chemistry; D024043:Collagen Type II / Q000235:genetics; D003692:Delayed-Action Preparations / Q000737:chemistry; D015870:Gene Expression; D006801:Humans; D008863:Microspheres; D010003:Osteoarthritis / Q000188:drug therapy / Q000378:metabolism; D054457:Tissue Scaffolds / Q000737:chemistry
D053674:Aggrecans; D023181:Antimicrobial Cationic Peptides; D055419:Bone Morphogenetic Protein 7; D024042:Collagen Type I; D024043:Collagen Type II; D003692:Delayed-Action Preparations; C045866:peptide-Gly-Leu-amide