Abscinazole-E1, a novel chemical tool for exploring the role of ABA 8'-hydroxylase CYP707A.
We developed abscinazole-E1 (Abz-E1), a specific inhibitor of abscisic acid (ABA) 8'-hydroxylase (CYP707A). This inhibitor was designed and synthesized as an enlarged analogue of uniconazole (UNI), a well-known plant growth retardant, which inhibits a gibberellin biosynthetic enzyme (ent-kaurene oxidase, CYP701A) as well as CYP707A. Our results showed that Abz-E1 functions as a potent inhibitor of CYP707A and a poor inhibitor of CYP701A both in vitro and in vivo. Abz-E1 application to plants resulted in improved desiccation tolerance and an increase in endogenous ABA.
Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, Shizuoka 422-8529, Japan.
Mariko
M
Okazaki
Hataitip
H
Nimitkeatkai
Taku
T
Muramatsu
Hikaru
H
Aoyama
Kotomi
K
Ueno
Masaharu
M
Mizutani
Nobuhiro
N
Hirai
Satoru
S
Kondo
Toshiyuki
T
Ohnishi
Yasushi
Y
Todoroki
D017360:Arabidopsis / Q000201:enzymology / Q000502:physiology; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry* / Q000378:metabolism*; D018525:Germination; D012275:Oryza / Q000201:enzymology / Q000502:physiology; D010940:Plant Proteins; D014230:Triazoles / Q000737:chemistry*
D010940:Plant Proteins; D014230:Triazoles; C557986:abscinazole E1; D003577:Cytochrome P-450 Enzyme System; C107203:abscisic acid 8'-hydroxylase