Human duodenal proteome modulations by glutamine and antioxidants.
OBJECTIVE
Glutamine (Gln) has protective, anti-inflammatory effects in animal models and humans. Antioxidant nutrients may exert synergistic effects on intestinal functions. Therefore, these combined nutrients may have a therapeutic potential during intestinal inflammation. This study was designed to investigate in humans the effects of a supplement composed of Gln and high-dosed antioxidant micronutrients compared to isomolar Gln only, on duodenal proteome.


METHODS
Enteral perfusion of Gln (0.8  mmol  x  kg(-1) x  h(-1)) or supplement was performed in two groups of six healthy volunteers during 5  h before taking endoscopic duodenal biopsies. Protein expression was analyzed by 2-DE and the relevant proteins identified by MS/MS.


RESULTS
About 1500 protein spots were revealed in both supplement and Gln conditions. Comparative proteomics analysis indicated that 11 proteins were differentially and significantly (p≤0.05) expressed in response to the supplement. These proteins were essentially implicated in metabolism pathways, e.g. fatty acid binding protein-1 and 40S ribosomal protein SA expressions were downregulated while manganese superoxide dismutase and retinal dehydrogenase-1 expressions were upregulated.


CONCLUSIONS
This study provides new information on human duodenal proteome and its nutritional modulation, and supports further clinical investigations designed to evaluate the effects of Gln plus antioxidants during intestinal inflammation and cancer.
Groupe ADEN EA4311, CIC 0204, Institut hospitalo-universitaire de recherche biomédicale, IFRMP23, Université et CHU de Rouen, Rouen France.
Sandrine
S
Thébault
Nicolas
N
Deniel
Alexandra
A
Galland
Stéphane
S
Lecleire
Roland
R
Charlionet
Moïse
M
Coëffier
François
F
Tron
David
D
Vaudry
Pierre
P
Déchelotte
D000328:Adult; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D019587:Dietary Supplements; D004305:Dose-Response Relationship, Drug; D004386:Duodenum / Q000187:drug effects* / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D005973:Glutamine / Q000494:pharmacology*; D006801:Humans; D008297:Male; D018977:Micronutrients / Q000494:pharmacology; D010865:Pilot Projects; D020543:Proteome / Q000378:metabolism*; D055815:Young Adult
D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D018977:Micronutrients; D020543:Proteome; D005973:Glutamine