Molluscan death effector domain (DED)-containing caspase-8 gene from disk abalone (Haliotis discus discus): molecular characterization and expression analysis.
The caspase family represents aspartate-specific cysteine proteases that play key roles in apoptosis and immune signaling. In this study, we cloned the first death effector domain (DED)-containing molluscan caspase-8 gene from disk abalone (Haliotis discus discus), which is named as hdCaspase-8. The full-length hdCaspase was 2855 bp, with a 1908 bp open reading frame encoding 636 amino acids. The hdCaspase-8 had 72 kDa predicted molecular mass with an estimated isoelectric point (PI) of 6.0. The hdCaspase-8 amino acid sequence contained the characteristic feature of an N-terminal two DED, a C-terminal catalytic domain and the caspase family cysteine active site ⁵¹³KPKLFFLQACQG⁵²⁴. Phylogenetic analysis results showed that hdCaspase-8 is more similar to the invertebrate Tubifex tubifex (sludge worm) caspase-8. Real-time RT-PCR results showed that hdCaspase-8 constitutively and ubiquitously expressed in all tested tissue of unchallenged disk abalone. The basal expression level of hdCaspase-8 in gill tissue was higher than all other tested tissues. The hdCaspase-8 mRNA expression in gill and hemocytes was significantly up-regulated by exposure to bacteria (Vibrio alginolyticus, Vibrio parahemolyticus and Listeria monocytogenes) and VHSV (viral hemorrhagic septicemia virus), as compared to control animals. These results suggest that hdCaspase-8 may be involved in immune response reactions in disk abalone.
Department of Marine Life Sciences, School of Marine Biomedical Sciences, Jeju National University, Jeju Special Self-Governing Province 690-756, Republic of Korea.
Youngdeuk
Y
Lee
Mahanama
M
De Zoysa
Ilson
I
Whang
Sukkyoung
S
Lee
Yucheol
Y
Kim
Chulhong
C
Oh
Cheol Young
CY
Choi
Sang-Yeob
SY
Yeo
Jehee
J
Lee
D000595:Amino Acid Sequence; D000818:Animals; D001419:Bacteria / Q000276:immunology; D001483:Base Sequence; D053181:Caspase 8 / Q000737:chemistry / Q000235:genetics* / Q000276:immunology*; D049851:Gastropoda / Q000145:classification / Q000201:enzymology* / Q000235:genetics* / Q000382:microbiology / Q000821:virology; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic; D005880:Gills / Q000201:enzymology; D006434:Hemocytes / Q000201:enzymology; D008969:Molecular Sequence Data; D029141:Novirhabdovirus / Q000276:immunology; D010802:Phylogeny; D016415:Sequence Alignment
D053181:Caspase 8