[Establishment of rat integrated discrete multiple organ cell culture (IdMOC) model].
OBJECTIVE
To establish the integrated discrete multiple organ cell culture (IdMOC) system.


METHODS
Rat primary cell of hepatocyte, nephrocyte, cardiomyocytes, alveolar macrophage, dermal fibroblasts were isolated by collagenase digestion, separation of bronchial lavage, two-step digestion method and cultured respectively, with monolayer culture. To establish the integrated discrete multiple organ cell culture (IdMOC) system, glass slides of five different cells were used to the same dish with 10% FBS DMEM medium cultured 7d, using MTT comparison primary cells cultured alone and cocultured when growth.


RESULTS
Established rat hepatocytes, renal cell, cardiomyocyte, alveolar macrophages, dermal fibroblasts separation method was stable, cell separation survival rate was about 90.0%. Hepatocytes separation survival rate 90.3% ,renal cell separation survival rate 91.9%, cardiomyocyte separation survival rate 93.0% and beating rate indifference curve among 3d-15d, alveolar macrophages cell separation survival rate 90.8%, dermal fibroblasts cell separation survival rate 92.7%. Five primary cells multiple organ cells coculture showed cocultured cell growth proliferation well, cultured alone and cocultured cells growth curve basic coincide.


CONCLUSIONS
Established rat multiple organ cell co-culture is successful.
Department of Toxicology, Second Military Medicine University, Shanghai 200433, China.
Gui-feng
GF
Xu
Jiang-bo
JB
Zhu
Yi-wen
YW
Zheng
Yu-ping
YP
Zhu
Xi-li
XL
Ma
Zhen
Z
Liu
Juan
J
Hou
Fei
F
Wang
Tian-bao
TB
Zhang
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D004847:Epithelial Cells / Q000166:cytology; D022781:Hepatocytes / Q000166:cytology; D016676:Macrophages, Alveolar / Q000166:cytology; D032383:Myocytes, Cardiac / Q000166:cytology; D051381:Rats; D017207:Rats, Sprague-Dawley