Comparative proteomic analysis of differentially expressed proteins in primary retinoblastoma tumors.
OBJECTIVE
To understand the disease mechanism and to identify the potential tumor markers that would help in therapeutics, comparative proteomic analysis of 29 retinoblastoma (RB) tumors was performed using 14 non-neoplastic retinas (age ranged from 45 to 89 years) as control tissues.


METHODS
2-DE and MALDI-TOF-TOF MS/MS were used to identify differentially expressed proteins.


RESULTS
Twenty-seven distinct differentially expressed proteins were identified, including 16 upregulated 11 downregulated proteins. Significantly, higher mRNA levels of apolipoprotein A1 (p<0.001), transferrin (TF; p<0.001), CRABP2 (p<0.001), α-crystallin A (CRYAA; p<0.001) were observed in RBs when compared with normal retinas and hence are consistent with the proteomic data. Immunohistochemistry was also performed for selected proteins on paraffin RB blocks to confirm protein expression. RB with invasion showed significantly higher expression by 2-DE-MS/MS analysis of CRABP2 (p<0.001), peroxiredoxin 6 (p=0.025), apolipoprotein A1 (p<0.001), recoverin (p<0.001).


CONCLUSIONS
Thus, this study provides a dynamic protein profile of RB tumors, which could provide clues to study the mechanisms of RB oncogenesis and possibly be developed as potential biomarkers for prognosis and therapy.
Department of Ocular Pathology, Vision Research Foundation, Sankara Nethralaya, Chennai, India.
Kandalam
K
Mallikarjuna
Curam Sreenivasacharlu
CS
Sundaram
Yogendra
Y
Sharma
Perinkulam Ravi
PR
Deepa
Vikas
V
Khetan
Lingam
L
Gopal
Jyotirmay
J
Biswas
Tarun
T
Sharma
Subramanian
S
Krishnakumar
D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000097:blood; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D040901:Proteomics; D012175:Retinoblastoma / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D014408:Biomarkers, Tumor