Hydrophobic amino acids at the cytoplasmic ends of helices 3 and 6 of rhodopsin conjointly modulate transducin activation.
Rhodopsin is the visual photoreceptor responsible for dim light vision. This receptor is located in the rod cell of the retina and is a prototypical member of the G-protein-coupled receptor superfamily. The structural details underlying the molecular recognition event in transducin activation by photoactivated rhodopsin are of key interest to unravel the molecular mechanism of signal transduction in the retina. We constructed and expressed rhodopsin mutants in the second and third cytoplasmic domains of rhodopsin--where the natural amino acids were substituted by the human M3 acetylcholine muscarinic receptor homologous residues--in order to determine their potential involvement in G-protein recognition. These mutants showed normal chromophore formation and a similar photobleaching behavior than WT rhodopsin, but decreased thermal stability in the dark state. The single mutant V138³·⁵³ and the multiple mutant containing V227⁵·⁶² and a combination of mutations at the cytoplasmic end of transmembrane helix 6 caused a reduction in transducin activation upon rhodopsin photoactivation. Furthermore, combination of mutants at the second and third cytoplasmic domains revealed a cooperative role, and partially restored transducin activation. The results indicate that hydrophobic interactions by V138³·⁵³, V227⁵·⁶², V250⁶·³³, V254⁶·³⁷ and I255⁶·³⁸ are critical for receptor activation and/or efficient rhodopsin-transducin interaction.
Centre de Biotecnologia Molecular, Departament d'Enginyeria Química, Universitat Politècnica de Catalunya, 08222 Terrassa, Spain.
Laia
L
Bosch-Presegué
Laura
L
Iarriccio
Mònica
M
Aguilà
Darwin
D
Toledo
Eva
E
Ramon
Arnau
A
Cordomí
Pere
P
Garriga
D019943:Amino Acid Substitution; D000596:Amino Acids / Q000737:chemistry; D000818:Animals; D002417:Cattle; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D066298:In Vitro Techniques; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D050505:Mutant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D054730:Protein Interaction Domains and Motifs; D055550:Protein Stability; D017433:Protein Structure, Secondary; D043587:Receptor, Muscarinic M3 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012243:Rhodopsin / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D013053:Spectrophotometry; D015293:Transducin / Q000378:metabolism*
D000596:Amino Acids; D050505:Mutant Proteins; D043587:Receptor, Muscarinic M3; D011994:Recombinant Proteins; D012243:Rhodopsin; D015293:Transducin