[Pseudoanaphylactoid reaction analysis of Chinese herbal injections in Beagle dogs].
OBJECTIVE
To investigate the preclinical evaluation method of pseudoanaphylactoid reactions for Chinese herbal injections.


METHODS
Beagle dogs were divided into control group (C), 0.5% tween 80 group (T), Yuxincao injection containing 0.5% tween 80 (YT), distilled solution from Yuxincao (Y). Various groups of Beagle dogs were given 3 mL x kg(-1) of the test articles intravenously. The anaphylactoid reactions were observed immediately, while blood pressure, respiratory frequencies and heart rates were tested at 10 min and 30 min after administration.


RESULTS
A variety of symptoms that range from cutaneous and mucosa signs to bronchospasma and cardiovascular collapse, including angioedema at lip, conjunctiva, ear and circumoral skin, somnolence, lethargy, breathless or dyspnea, severe hypotension etc were observed in T and TY groups from immediately post-injection to at least 30 min after administration. These reactions occurred at both first injection or repeated injections at 24 weeks intervals, manifesting that it was pseudoanaphylactoid reaction mediated by non-immune mechanisms.


CONCLUSIONS
Beagle dogs could be used as an animal model for preclinical evaluation of pseudoanaphylactoid reactions of Chinese herbal injection with sensitivity, reproducibility, and high clinic consistency.
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China. liangaihua@sina.com
Aihua
A
Liang
Chunying
C
Li
Ran
R
Hao
Chunyu
C
Cao
Yan
Y
Yi
Ting
T
Liu
Hua
H
Yang
Hong
H
Yi
Jing
J
Guo
D000707:Anaphylaxis / Q000139:chemically induced / Q000209:etiology* / Q000276:immunology*; D000818:Animals; D004285:Dogs; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000009:adverse effects*; D007275:Injections, Intravenous; D008297:Male; D023421:Models, Animal; D011897:Random Allocation
D004365:Drugs, Chinese Herbal