Effect of recombinant osteopontin on cerebral vasospasm after subarachnoid hemorrhage in rats.
BACKGROUND
osteopontin (OPN), a pleiotropic extracellular matrix glycoprotein, has been reported to have neuroprotective effects against early brain injury after subarachnoid hemorrhage (SAH). The aim of this study is to examine if osteopontin prevents cerebral vasospasm after SAH in rats.


METHODS
the endovascular perforation model of SAH was produced, and 62 rats were randomly assigned to sham + vehicle, SAH + vehicle, and SAH+ r-OPN (0.1 microg) groups. Cerebral vasospasm was evaluated by India ink angiography at 24 and 72 h after SAH, as well as neurobehavioral tests.


RESULTS
significant vasospasm and neurological impairments occurred over the observed period after SAH. r-OPN significantly prevented vasospasm in the left middle cerebral artery at 24 h and improved neurological impairments at 48 h after SAH. In other time points studied, r-OPN had a tendency toward improving both vasospasm and neurological scores, but the difference was not significant.


CONCLUSIONS
this study shows that r-OPN has anti-vasospastic effects against cerebral vasospasm after SAH.
Department of Physiology, Loma Linda University School of Medicine, Risley Hall, Room 223, Loma Linda, CA, USA.
Hidenori
H
Suzuki
Yu
Y
Hasegawa
Kenji
K
Kanamaru
John H
JH
Zhang
D000704:Analysis of Variance; D000792:Angiography / Q000379:methods; D000818:Animals; D002244:Carbon; D004195:Disease Models, Animal; D008297:Male; D020768:Middle Cerebral Artery / Q000187:drug effects / Q000473:pathology; D009460:Neurologic Examination; D053495:Osteopontin / Q000096:biosynthesis / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000627:therapeutic use*; D012720:Severity of Illness Index; D013345:Subarachnoid Hemorrhage / Q000150:complications; D013997:Time Factors; D020301:Vasospasm, Intracranial / Q000188:drug therapy* / Q000209:etiology / Q000473:pathology
D011994:Recombinant Proteins; C028433:chinese ink; D053495:Osteopontin; D002244:Carbon