Kinetics and mechanism of NO production in the Ru(III)-(edta) mediated oxidation of L-arginine with H(2)O(2).
The kinetics of the Ru(III)-(edta) (edta(4-) = ethylenediaminetetraacetate) catalyzed oxidation of l-arginine by H(2)O(2) mimicking the action of nitric oxide synthases (NOSs) has been studied spectrophotometrically. The time course of the reaction of [Ru(V)(edta)O](-) with l-arginine was followed at 390 nm under catalytic turn-over conditions. Formation of NO in the reacting system has been confirmed with an isolated nitric oxide free radical analyzer. A detailed reaction mechanism in agreement with the spectral and kinetic data is presented.
Chemistry Group, Central Mechanical Engineering Research Institute, CSIR, MG Avenue, Durgapur, 713209, India. dchat57@hotmail.com
Debabrata
D
Chatterjee
Sarita
S
Ghosh
Ujjwal
U
Pal
D001120:Arginine / Q000737:chemistry*; D002384:Catalysis; D056831:Coordination Complexes / Q000737:chemistry*; D004492:Edetic Acid / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry*; D007700:Kinetics; D009569:Nitric Oxide / Q000737:chemistry*; D019001:Nitric Oxide Synthase / Q000378:metabolism; D010084:Oxidation-Reduction; D012428:Ruthenium / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
D056831:Coordination Complexes; D009569:Nitric Oxide; D012428:Ruthenium; D001120:Arginine; D004492:Edetic Acid; D006861:Hydrogen Peroxide; D019001:Nitric Oxide Synthase