Comparison of recombinant protein expression in a baculovirus system in insect cells (Sf9) and silkworm.
Using a hybrid baculovirus system, we compared the expression of 45 recombinant proteins from six categories using two models: silkworm (larvae and pupae) and an Sf9 cell line. A total of 45 proteins were successfully expressed; preparation of hybrid baculovirus was unsuccessful for one protein, and two proteins were not expressed. A similar pattern of expression was seen in both silkworm and Sf9 cells, with double and multiple bands found in immunoblotting of the precipitate of both hosts. Degraded proteins were seen only in the silkworm system (particularly in the larvae). Production was more efficient in silkworms; a single silkworm produced about 70 times more protein than 10(6) Sf9 cells in 2 ml of culture medium.
Katakura Industries Co. Ltd, Research Institute of Biological Science, Sayama, Saitama, Japan.
Akihiro
A
Usami
Seiji
S
Ishiyama
Chiaki
C
Enomoto
Hironobu
H
Okazaki
Keiko
K
Higuchi
Mashahiro
M
Ikeda
Takeshi
T
Yamamoto
Mutsumi
M
Sugai
Yukiko
Y
Ishikawa
Yumiko
Y
Hosaka
Teruyuki
T
Koyama
Yoneko
Y
Tobita
Syoko
S
Ebihara
Toshiko
T
Mochizuki
Yoshimi
Y
Asano
Hidekazu
H
Nagaya
D000818:Animals; D016367:Baculoviridae / Q000737:chemistry / Q000235:genetics*; D001709:Biotechnology / Q000379:methods; D012831:Bombyx / Q000235:genetics / Q000378:metabolism / Q000821:virology*; D002460:Cell Line; D046528:Chimerism; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005818:Genetic Engineering / Q000379:methods; D006801:Humans; D015151:Immunoblotting; D007814:Larva / Q000235:genetics / Q000378:metabolism / Q000821:virology*; D010446:Peptide Fragments / Q000096:biosynthesis / Q000235:genetics; D011679:Pupa / Q000235:genetics / Q000378:metabolism / Q000821:virology*; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics; D018411:Spodoptera / Q000166:cytology / Q000235:genetics / Q000821:virology*
D010446:Peptide Fragments; D011994:Recombinant Proteins