Mechanisms involved in the antinociception of petroleum ether fraction from the EtOH extract of Chrysanthemum indicum in mice.
The petroleum ether fraction (PEF) from the EtOH extract of flowers and buds of Chrysanthemum indicum was evaluated on antinociception in mice using chemical and thermal models of nociception. PEF administered orally at doses of 188 and 376 mg/kg produced significant inhibitions on chemical nociception induced by intraperitoneal acetic acid, subplantar formalin or capsaicin injections and on thermal nociception in the tail-flick test and the hot plate test. In the pentobarbital sodium-induced sleep time test and the open-field test, PEF neither enhanced the pentobarbital sodium-induced sleep time nor impaired the motor performance, indicating that the observed antinociception was unrelated to sedation or motor abnormality. In a measurement of core body temperature, PEF did not affect temperature within 80 min. Moreover, PEF-induced antinociception in the capsaicin test was insensitive to naloxone, yohimbine or methylene blue, but was significantly antagonized by atropine and glibenclamide. These results suggested that PEF-produced antinociception might be involvement in the ATP sensitive K+ channels and the mAChRs-ATP sensitive K+ channels pathway. In additional, the antinociception of PEF might attribute to the synergic effects of these two compounds, 2-[[2-[2-[(2-ethylcyclopropyl)methyl] cyclop Cyclopropaneoctanoic and n-hexadecanoic acid, or the property of a single compound, which merited exploring further.
Department of Pharmacy, Shen-yang North Hospital, 83# Wenhua Road, Shen-yang 110840, PR China.
Guo-bing
GB
Shi
Ming-hong
MH
Zhao
Qing-chun
QC
Zhao
Ying
Y
Huang
Yu-feng
YF
Chen
D000255:Adenosine Triphosphate / Q000378:metabolism; D000473:Alkanes; D000700:Analgesics / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D001831:Body Temperature / Q000187:drug effects; D031202:Chrysanthemum / Q000737:chemistry*; D000431:Ethanol; D035264:Flowers / Q000737:chemistry; D051379:Mice; D008813:Mice, Inbred ICR; D009043:Motor Activity / Q000187:drug effects; D010146:Pain / Q000139:chemically induced / Q000188:drug therapy; D010147:Pain Measurement / Q000187:drug effects / Q000379:methods; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D015221:Potassium Channels / Q000378:metabolism*; D011930:Reaction Time / Q000187:drug effects; D012890:Sleep / Q000187:drug effects; D012997:Solvents
D000473:Alkanes; D000700:Analgesics; D010936:Plant Extracts; D015221:Potassium Channels; D012997:Solvents; D000431:Ethanol; D000255:Adenosine Triphosphate; C004544:naphtha