Analysis of variable sites between two complete South China tiger (Panthera tigris amoyensis) mitochondrial genomes.
In order to investigate the mitochondrial genome of Panthera tigris amoyensis, two South China tigers (P25 and P27) were analyzed following 15 cymt-specific primer sets. The entire mtDNA sequence was found to be 16,957 bp and 17,001 bp long for P25 and P27 respectively, and this difference in length between P25 and P27 occurred in the number of tandem repeats in the RS-3 segment of the control region. The structural characteristics of complete P. t. amoyensis mitochondrial genomes were also highly similar to those of P. uncia. Additionally, the rate of point mutation was only 0.3% and a total of 59 variable sites between P25 and P27 were found. Out of the 59 variable sites, 6 were located in 6 different tRNA genes, 6 in the 2 rRNA genes, 7 in non-coding regions (one located between tRNA-Asn and tRNA-Tyr and six in the D-loop), and 40 in 10 protein-coding genes. COI held the largest amount of variable sites (9 sites) and Cytb contained the highest variable rate (0.7%) in the complete sequences. Moreover, out of the 40 variable sites located in 10 protein-coding genes, 12 sites were nonsynonymous.
The Sichuan Key Laboratory of Conservation Biology on Endangered Wildlife, Chengdu Research Base of Giant Panda Breeding, Chengdu, 610081 Sichuan, People's Republic of China. zhang_zoology@163.com
Wenping
W
Zhang
Bisong
B
Yue
Xiaofang
X
Wang
Xiuyue
X
Zhang
Zhong
Z
Xie
Nonglin
N
Liu
Wenyuan
W
Fu
Yaohua
Y
Yuan
Daqing
D
Chen
Danghua
D
Fu
Bo
B
Zhao
Yuzhong
Y
Yin
Xiahui
X
Yan
Xinjing
X
Wang
Rongying
R
Zhang
Jie
J
Liu
Maoping
M
Li
Yao
Y
Tang
Rong
R
Hou
Zhihe
Z
Zhang
D000818:Animals; D001482:Base Composition / Q000235:genetics; D001483:Base Sequence; D002467:Cell Nucleus / Q000235:genetics; D002681:China; D004272:DNA, Mitochondrial / Q000235:genetics*; D054629:Genome, Mitochondrial / Q000235:genetics*; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D046010:Tigers / Q000235:genetics*
D004272:DNA, Mitochondrial