[Determination of matrine in matrine injection by microfluidic chip with contactless conductivity detection].
OBJECTIVE
To establish a new method for the determination of matrine in matrine injection by microfluidic chip with contactless conductivity detection.


METHODS
The electrophoretic parameters, such as the variety and concentration of buffer solution and additive, separation voltage and injection time, etc. were researched.


RESULTS
In the optimal conditions, using 2 mmol/L HAc + 2 mmol/L NaAc (pH 4. 5) as buffer solution, with 0.20 mmol/L SDS as addition at the separation voltage of 2.00 kV and 10s injection, matrine was separated and detected within 2 min. The linear dependence of the concentration of matrine ranged from 10 to 300 microg/mL (r2 = 0.9992), with the detection limit of 1.0 microg/mL (S/N = 3), the RSD of 1.9% and the recoveries from 98.7% to 101%.


CONCLUSIONS
The method is simple, rapid and well reproducible, and it can be used in the quality control of the product.
Guangdong Food and Drug Vocational College, Guangzhou 510520, China. wangc@gdyzy.edu.cn
Chong
C
Wang
Yong-Chong
YC
Li
Zi-You
ZY
Cai
Bin
B
Ye
Zuan-Guang
ZG
Chen
D000470:Alkaloids / Q000032:analysis*; D002021:Buffers; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000737:chemistry*; D004553:Electric Conductivity; D046209:Electrophoresis, Microchip / Q000379:methods; D006863:Hydrogen-Ion Concentration; D007267:Injections; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D011786:Quality Control; D011807:Quinolizines / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D029909:Sophora / Q000737:chemistry; D013997:Time Factors; D000093842:Matrines
D000470:Alkaloids; D002021:Buffers; D004365:Drugs, Chinese Herbal; D011807:Quinolizines; D000093842:Matrines