Diagnostic protein marker patterns in squamous cervical cancer.
OBJECTIVE
Cervical cancer is the second most prevalent malignancy of women. Our aim was to identify additional marker protein patterns for objective diagnosis of squamous cervical cancer (SCC).


METHODS
Collected tissue biopsies of SCC, squamous vaginal cancer (SVC), normal cervical and vaginal mucosa were subjected to 2-DE, SameSpot analysis, MALDI-TOF-MS protein identification, and analysis of the expression of selected proteins by immunohistochemistry.


RESULTS
In 148 protein spots selected by the difference in expression 99 proteins were identified. A differential protein pattern for SCC was, e.g. over-expressed (OE) eukaryotic translation initiation factor 3-2β, neutrophil cytosolic factor 2, annexin A6 (ANXA6), for SVC it was OE cathepsin D, γ-catenin, RAB2A, for both cancers it was OE apolipoprotein E, tropomyosin 3, HSPA8, and underexpressed cytokeratin 13, osteoglycin. In SCC nuclear expression of neutrophil cytosolic factor 2, PRDX2, HSP27 (nine of ten cases), ANXA6 (nine of ten cases) was observed while tropomyosin 4 was expressed only in two of ten cases. There was 81.1% (43/53) agreement between the expression of protein spots and the immune expression of proteins (www.proteinatlas.org).


CONCLUSIONS
SCC is characterized by specific tissue marker protein patterns that allow objective detection of the disease. They can become a basis for objective automated cytology-based screening and improve current diagnostics of SCC.
Department of Obstetrics and Gynecology, Institute for Clinical Science and Technology, Karolinska University Hospital, Huddinge, Karolinska Institutet, Stockholm, Sweden. marta.lomnytska@ki.se
Marta I
MI
Lomnytska
Susanne
S
Becker
Kristina
K
Hellman
Ann-Cathrin
AC
Hellström
Serhiy
S
Souchelnytskyi
Miriam
M
Mints
Ulf
U
Hellman
Sonia
S
Andersson
Gert
G
Auer
D014408:Biomarkers, Tumor / Q000302:isolation & purification / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D018307:Neoplasms, Squamous Cell / Q000175:diagnosis* / Q000235:genetics / Q000378:metabolism*; D020543:Proteome / Q000302:isolation & purification / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D015203:Reproducibility of Results; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000378:metabolism*
D014408:Biomarkers, Tumor; D020543:Proteome