Tight glycaemic control is a key factor in wound healing enhancement strategies in an experimental diabetes mellitus model.
BACKGROUND
Diabetes mellitus is a leading cause of impaired wound healing. The aim of this study was to establish a glucose-controlled diabetic wound healing model.


METHODS
Sprague-Dawley rats were divided into three groups: Control group (C), Diabetic Non-glucose Controlled group (DNC) and Diabetic glucose Controlled group (DC).


RESULTS
Glucose control was achieved using Insulman Rapid (average daily glucose level <10 mmol/L). 18 Sprague-Dawley rats underwent a dorsal skin wound incision and 10 days later were killed. Fresh and fixed wound tensile strength, hydroxyproline and transforming growth factor beta-1 levels were improved in the DC group when compared to the DNC group. The quantity of fibroblasts present was similar in each group.


CONCLUSIONS
This study demonstrates the impact that diabetes has on acute wound healing and suggests that wound modulating agents must be tested in both the tightly glucose-controlled as well as the poorly glucose-controlled diabetic animal models prior to proceeding with translational clinical studies.
Department of Surgery, Royal College of Surgeons in Ireland, Beaumont Hospital, Dublin, Ireland. jbarryosullivan@mac.com
J B
JB
O'Sullivan
R
R
Hanson
F
F
Chan
D J
DJ
Bouchier-Hayes
D000818:Animals; D001786:Blood Glucose / Q000032:analysis*; D001826:Body Fluids / Q000737:chemistry; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000503:physiopathology*; D005347:Fibroblasts / Q000378:metabolism; D006909:Hydroxyproline / Q000032:analysis; D007150:Immunohistochemistry; D008297:Male; D023421:Models, Animal; D051381:Rats; D017207:Rats, Sprague-Dawley; D013718:Tensile Strength; D053773:Transforming Growth Factor beta1 / Q000032:analysis; D014945:Wound Healing / Q000502:physiology*
D001786:Blood Glucose; D053773:Transforming Growth Factor beta1; D006909:Hydroxyproline