Methylogaea oryzae gen. nov., sp. nov., a mesophilic methanotroph isolated from a rice paddy field.
A novel methanotroph, designated strain E10(T), was isolated from a rice paddy field in Uruguay. Strain E10(T) grew on methane and methanol as sole carbon and energy sources. Cells were Gram-negative, non-motile, non-pigmented, slightly curved rods showing type I intracytoplasmic membranes arranged in stacks. The strain was neutrophilic and mesophilic; optimum growth occurred at 30-35 °C with no growth above 37 °C. The strain possessed only a particulate methane monooxygenase (pmoA). Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain was most closely related to the moderately thermophilic strains Methylocaldum szegediense OR2(T) (91.6 % sequence similarity) and Methylococcus capsulatus Bath (91.5 %). Comparative sequence analysis of pmoA genes also confirmed that strain E10(T) formed a new lineage among the genera Methylocaldum and Methylococcus with 89 and 84 % derived amino acid sequence identity to Methylococcus capsulatus Bath and Methylocaldum gracile VKM-14L(T), respectively. The DNA G+C content was 63.1 mol% and the major cellular fatty acid was C(16 :0) (62.05 %). Thus, strain E10(T) (=JCM 16910(T) = DSM 23452(T)) represents the type strain of a novel species within a new genus, for which the name Methylogaea oryzae gen. nov., sp. nov. is proposed.
Cátedra de Microbiología, Facultad de Química y Facultad de Ciencias, Universidad de la República-UDELAR, CC1157 Montevideo, Uruguay.
Estefanía
E
Geymonat
Cátedra de Microbiología, Facultad de Química y Facultad de Ciencias, Universidad de la República-UDELAR, CC1157 Montevideo, Uruguay.
Lucía
L
Ferrando
Cátedra de Microbiología, Facultad de Química y Facultad de Ciencias, Universidad de la República-UDELAR, CC1157 Montevideo, Uruguay.
Silvana E
SE
Tarlera
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D015373:Bacterial Typing Techniques; D001482:Base Composition; D005227:Fatty Acids / Q000378:metabolism; D000432:Methanol / Q000378:metabolism*; D008772:Methylococcaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D008969:Molecular Sequence Data; D012275:Oryza / Q000382:microbiology*; D010105:Oxygenases / Q000235:genetics / Q000378:metabolism; D010802:Phylogeny; D012988:Soil Microbiology
D001426:Bacterial Proteins; D005227:Fatty Acids; D010105:Oxygenases; C021555:methane monooxygenase; D000432:Methanol