Systemic central venous oxygen saturation is associated with clot strength during traumatic hemorrhagic shock: A preclinical observational model.
BACKGROUND
Clot strength by Thrombelastography (TEG) is associated with mortality during trauma and has been linked to severity of tissue hypoperfusion. However, the optimal method for monitoring this important relationship remains undefined. We hypothesize that oxygen transport measurements will be associated with clot strength during traumatic shock, and test this hypothesis using a swine model of controlled traumatic shock.


METHODS
N = 33 swine were subjected to femur fracture and hemorrhagic shock by controlled arterial bleeding to a predetermined level of oxygen debt measured by continuous indirect calorimetry. Hemodynamics, oxygen consumption, systemic central venous oxygenation (ScvO₂), base excess, lactate, and clot maximal amplitude by TEG (TEG-MA) as clot strength were measured at baseline and again when oxygen debt = 80 ml/kg during shock. Oxygen transport and metabolic markers of tissue perfusion were then evaluated for significant associations with TEG-MA. Forward stepwise selection was then used to create regression models identifying the strongest associations between oxygen transport and TEG-MA independent of other known determinants of clot strength.


RESULTS
Multiple markers of tissue perfusion, oxygen transport, and TEG-MA were all significantly altered during shock compared to baseline measurements (p < 0.05). However, only ScvO₂ demonstrated a strong bivariate association with TEG-MA measured during shock (R = 0.7, p < 0.001). ScvO₂ measured during shock was also selected by forward stepwise selection as an important covariate in linear regression models of TEG-MA after adjusting for the covariates fibrinogen, pH, platelet count, and hematocrit (Whole model R² = 0.99, p ≤ 0.032).


CONCLUSIONS
Among multiple measurements of oxygen transport, only ScvO₂ was found to retain a significant association with TEG-MA during shock after adjusting for multiple covariates. ScvO₂ should be further studied for its utility as a clinical marker of both tissue hypoxia and clot formation during traumatic shock.
Reanimation Engineering Science Center, Virginia Commonwealth University, Richmond, 23298, USA. whiten4@u.washington.edu
Nathan J
NJ
White
Erika J
EJ
Martin
Yongyun
Y
Shin
Donald F
DF
Brophy
Robert F
RF
Diegelmann
Kevin R
KR
Ward
D000818:Animals; D001692:Biological Transport; D001777:Blood Coagulation / Q000502:physiology*; D001784:Blood Gas Analysis; D002405:Catheterization, Central Venous; D004195:Disease Models, Animal; D005264:Femoral Fractures / Q000097:blood; D006439:Hemodynamics; D008297:Male; D010100:Oxygen / Q000097:blood*; D010101:Oxygen Consumption; D012771:Shock, Hemorrhagic / Q000097:blood*; D012774:Shock, Traumatic / Q000097:blood*; D013552:Swine; D013916:Thrombelastography
D010100:Oxygen