Detection, quantification, and characterisation of HIV/SIV.
Selected techniques for the detection, quantification, and characterisation of HIV1, HIV2, and SIV, as applied to diagnostic and research purposes, are described. Representative nucleic acid testing protocols including nested PCR, RT-PCR, and quantitative real-time PCR, as well as protocols based on virus infectivity, are presented.
Division of Cellular & Molecular Medicine, Centre for Infection, St. George's University of London, London, UK.
Neil
N
Berry
Carolina
C
Herrera
Martin
M
Cranage
D000818:Animals; D006678:HIV / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D015658:HIV Infections / Q000175:diagnosis* / Q000821:virology; D015497:HIV-1 / Q000235:genetics / Q000302:isolation & purification; D015498:HIV-2 / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D016097:Simian Acquired Immunodeficiency Syndrome / Q000175:diagnosis* / Q000821:virology; D015302:Simian Immunodeficiency Virus / Q000302:isolation & purification*