Detection of bacteria using glyco-dendronized polylysine prepared by continuous flow photofunctionalization.
Biocompatible glyco-dendronized poly-l-lysine (PLL) polymers carry either three or nine mannose- or galactose-bearing dendrons that selectively bind, and thus can be used to detect, bacteria. Central to the synthesis of glyco-dendronized polymers was the development of a continuous flow [2 + 2] photocycloaddition reaction to connect the dendrons and PLL. Glycodendronized polymers cluster bacteria by binding to cell-surface carbohydrate receptors and thereby result in an easy read-out using microscopic analyses.
Max Planck Institute for Colloids and Interfaces, Department of Biomolecular Systems, Research Campus Golm, 14476 Potsdam, Germany.
Paola
P
Laurino
Raghavendra
R
Kikkeri
Nahid
N
Azzouz
Peter H
PH
Seeberger
D015374:Biosensing Techniques / Q000379:methods*; D050091:Dendrimers / Q000737:chemistry* / Q000378:metabolism; D004926:Escherichia coli / Q000302:isolation & purification* / Q000378:metabolism; D005690:Galactose / Q000737:chemistry / Q000378:metabolism; D008358:Mannose / Q000737:chemistry* / Q000378:metabolism; D018625:Microscopy, Atomic Force; D008856:Microscopy, Fluorescence; D043942:Nanotubes / Q000737:chemistry / Q000648:ultrastructure; D010777:Photochemistry; D011107:Polylysine / Q000737:chemistry*; D012680:Sensitivity and Specificity
D050091:Dendrimers; D011107:Polylysine; D008358:Mannose; D005690:Galactose