Real-time PCR-based quantification of Toxoplasma gondii in tissue samples of serologically positive outdoor chickens.
This study aimed to quantify Toxoplasma gondii in tissue samples of serologically positive chickens using real-time polymerase chain reaction (PCR). Of 65 chickens evaluated, 28 were positive for T. gondii antibodies. Brain and heart samples were collected from 26 seropositive chickens and DNA was extracted using Trizol® and amplified using real-time PCR with SYBR® Green. Parasite DNA was detected in 24 of the 26 samples analyzed; the number of positive tissue samples and the parasite quantity did not differ between tissue types. The results confirmed the analytical sensitivity of parasite detection in chicken tissue samples and demonstrated the possibility of using other molecular systems for genotypic analysis.
Mestrado em Biotecnologia Aplicada à Agricultura, Universidade Paranaense, Umuarama, PR, Brasil.
Cleiton Paulo
CP
Aigner
Aristeu Vieira da
AV
Silva
Fabiano
F
Sandrini
Paulo de Sá
Pde S
Osório
Lilian
L
Poiares
Alvaro
A
Largura
D000818:Animals; D001921:Brain / Q000469:parasitology*; D002645:Chickens / Q000469:parasitology*; D016054:DNA, Protozoan / Q000097:blood*; D005838:Genotype; D006321:Heart / Q000469:parasitology*; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D014122:Toxoplasma / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*
D016054:DNA, Protozoan