Anti-pancreatic antibody in Turkish patients with inflammatory bowel disease and first-degree relatives.
OBJECTIVE
To identify the role of anti-pancreatic antibody (PAB) in the diagnosis of inflammatory bowel diseases (IBD) among Turkish patients, and its frequency in first-degree relatives.


METHODS
PAB and anti-Saccharomyces cerevisiae (ASCA) were examined in serum samples of 214 subjects including patients with Crohn's disease (CD, n = 64), ulcerative colitis (UC, n = 63), first-degree relatives of patients with CD (n = 25), first-degree relatives of patients with UC (n = 28),and a control group with gastrointestinal symptoms other than (IBD) (n = 34) by indirect immunofluorescence Positivity of PAB and ASCA was compared in terms of Vienna classification, disease activity and medications used.


RESULTS
In terms of PAB positivity, no difference was found between patients with CD (14.1%) and UC (7.9%) however, significant difference was observed between patients with CD and subjects in the control group (P < 0.05). No difference was found between patients with CD and their relatives in terms of ASCA positivity, whereas a significant difference was found between other groups (P < 0.001). Compared to ASCA, the sensitivity of the PAB was 19% (7/37), its specificity was 93% (25/27), positive predictive value was 77% (7/9) and negative predictive value was 45% (25/55). ASCA was found with significantly higher prevalence in patients with CD activity index > 150 (P < 0.05).


CONCLUSIONS
PAB is valuable in the diagnosis of IBD rather than CD, but cannot be used alone for diagnostic purposes. PAB is not superior to ASCA in CD diagnosis and in detecting CD among relatives of patients with CD.
Department of Gastroenterology, Sisli Etfal Training and Research Hospital, 34377 Istanbul, Turkey.
Huseyin
H
Demirsoy
Kamil
K
Ozdil
Ozdal
O
Ersoy
Besir
B
Kesici
Cetin
C
Karaca
Canan
C
Alkim
Nihat
N
Akbayir
Levent Kamil
LK
Erdem
Mehmet Derya
MD
Onuk
Hulya Tugrul
HT
Beyzadeoglu
D000293:Adolescent; D000328:Adult; D000907:Antibodies, Bacterial / Q000097:blood; D001323:Autoantibodies / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D016009:Chi-Square Distribution; D003093:Colitis, Ulcerative / Q000175:diagnosis / Q000235:genetics / Q000276:immunology* / Q000382:microbiology; D003424:Crohn Disease / Q000175:diagnosis / Q000235:genetics / Q000276:immunology* / Q000382:microbiology; D005190:Family; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D008297:Male; D008875:Middle Aged; D010179:Pancreas / Q000276:immunology*; D011237:Predictive Value of Tests; D012441:Saccharomyces cerevisiae / Q000276:immunology; D012680:Sensitivity and Specificity; D012720:Severity of Illness Index; D014421:Turkey; D055815:Young Adult
D000907:Antibodies, Bacterial; D001323:Autoantibodies; D015415:Biomarkers