Thermal treatment enhances the stability and biological activity of a truncated tilapia somatotropin contained in Pichia pastoris culture supernatant.
The importance of somatotropin as a growth promoting agent and immune-stimulator has long been recognized and its potential application in the fish farming industry has been an active research area. In the work reported here, we sought to improve the stability of a previously obtained truncated somatotropin by applying a 60 °C heat shock to the culture supernatant containing this molecule, and then compared its effects with and without heat shock on larval growth and immune functions. We observed that the treatment with heat shock at 60 °C enhanced protein stability, growth and innate immune functions in tilapia larvae.
Animal Biotechnology Division, Center for Genetic Engineering and Biotechnology, P.O. Box 6162, Havana 10600, Cuba.
Jannel
J
Acosta
Odalys
O
Ruiz
Yamila
Y
Carpio
Reynold
R
Morales
Julio C
JC
Aguila
Jorge
J
Valdés
Eduardo
E
Martínez
Mario P
MP
Estrada
D000818:Animals; D015153:Blotting, Western; D003470:Culture Media / Q000378:metabolism; D013006:Growth Hormone / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D006360:Heat-Shock Proteins / Q000737:chemistry; D018869:Heat-Shock Response; D006358:Hot Temperature; D037102:Lectins; D010843:Pichia / Q000378:metabolism*; D017382:Reactive Oxygen Species; D013696:Temperature; D017210:Tilapia; D013997:Time Factors
D003470:Culture Media; D006360:Heat-Shock Proteins; D037102:Lectins; D017382:Reactive Oxygen Species; D013006:Growth Hormone