Detection of bulky DNA lesions: DDB2 at the interface of chromatin and DNA repair in eukaryotes.
Bulky DNA damage is corrected by the nucleotide excision repair (NER) pathway. Although the core biochemical mechanism of NER is understood, details including lesion recognition and repair in the context of chromatin remain to be elucidated. As more data become available, the complexity of lesion recognition in chromatin is becoming clear. This review will discuss current knowledge of DNA damage recognition in the context of chromatin, with a focus on the roles of chromatin remodeling and the specific lesion recognition protein DDB2 (DNA damage-binding protein 2) in chromatin repair. Additionally, we propose a model that ubiquitination-mediated DDB2 dissociation from chromatin, not its degradation, is important for GG-NER progression.
Department of Biochemistry and Molecular Biology, University of Miami Miller School of Medicine, Miami, FL, USA.
Kristi L
KL
Jones
Ling
L
Zhang
Kenneth L
KL
Seldeen
Feng
F
Gong
D002843:Chromatin; D042002:Chromatin Assembly and Disassembly / Q000502:physiology*; D004249:DNA Damage; D004265:DNA Helicases / Q000502:physiology; D004260:DNA Repair / Q000502:physiology*; D045643:DNA Repair Enzymes / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism / Q000502:physiology*; D006657:Histones / Q000378:metabolism; D006801:Humans; D008958:Models, Molecular; D009687:Nuclear Proteins / Q000502:physiology; D012441:Saccharomyces cerevisiae / Q000235:genetics; D014157:Transcription Factors / Q000502:physiology; D054875:Ubiquitination
D002843:Chromatin; C117669:DDB2 protein, human; D004268:DNA-Binding Proteins; D006657:Histones; D009687:Nuclear Proteins; D014157:Transcription Factors; C086985:RAD23A protein, human; C086984:XPC protein, human; C084108:SMARCA4 protein, human; D004265:DNA Helicases; D045643:DNA Repair Enzymes