Inhibitory activities of anthraquinones from Rubia akane on phosphatase regenerating liver-3.
The methanolic extract of the roots of Rubia akane (Rubiaceae) was found to show inhibitory activity on phosphatase of regenerating liver-3 (PRL-3). Bioassay-guided fractionation of the methanolic extract resulted in the isolation of two anthraquinone compounds, 2-methyl-1,3,6-trihydroxy-9,10-anthraquinone-3-O-(6'-O-acetyl)-α-rhamnosyl(1→2)-β-glucoside and 2-methyl-1,3,6-trihydroxy-9,10-anthraquinone, as inhibitors on PRL-3. These compounds inhibited PRL-3 in a dose-dependent manner with IC₅₀ values of 5.2 and 1.3 μg/mL, respectively.
Woosuk University, Samrye, Korea.
Mi Kyeong
MK
Moon
Young-Min
YM
Han
Yu-Jin
YJ
Lee
Lan Hee
LH
Lee
Jae Heon
JH
Yang
Byoung-Mog
BM
Kwon
Dae Keun
DK
Kim
D000880:Anthraquinones / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D005960:Glucosides / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D009363:Neoplasm Proteins / Q000037:antagonists & inhibitors* / Q000378:metabolism; D018517:Plant Roots / Q000737:chemistry; D017027:Protein Tyrosine Phosphatases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D045470:Rubia / Q000737:chemistry*
C558507:2-methyl-1,3,6-trihydroxy-9,10-anthraquinone-3-O-(6'-O-acetyl)-alpha-rhamnosyl(1-2)-beta-glucoside; D000880:Anthraquinones; D000970:Antineoplastic Agents; D005960:Glucosides; D009363:Neoplasm Proteins; C484730:PTP4A3 protein, human; D017027:Protein Tyrosine Phosphatases