Wound healing activity of Glycosmis arborea leaf extract in rats.
BACKGROUND
Glycosmis arborea Roxb, syn. Glycosmis pentaphylla Retz, (Rutacea) is a shrub found all over India, have been used for the healing of wounds of livestock in Indian folk medicine.


OBJECTIVE
The present study provides a scientific evaluation for the wound healing potential of ethanolic extract of Glycosmis arborea leaves.


METHODS
Excision and incision wounds were inflicted upon four groups containing six rats each. Group-1 was assigned as control (ointment base), Group-2 and Group-3 were treated with 5 and 10% EtOH extract ointment. Group-4 was treated with standard Nitrofurazone (0.2%) ointment. The parameters observed were percentage of wound contraction, epithelization period, tensile strength, hydroxyproline content. Ethanolic extract of Glycosmis arborea leaves was given orally at a dose of 200, 400mg/kg/day in dead space wound model.


RESULTS
Topical application of Glycosmis arborea ointment (10%) in excision wound model significantly (P<0.05) increased the percentage of wound contraction (96.91%) compared with control (60.27%) and decreased the epithelization time. The tensile strength significantly (P<0.05) increased in Group-3 at 560.33 ± 6.48g when compared to control at 319.17±6.16 g. Rats treated with Glycosmis arborea extract (400mg/kg) showed significant (P<0.05) increase in hydroxyproline content at 54.94 ± 0.96 mg/g when compared with control at 30.77±1.13 mg/g.


CONCLUSIONS
The ethanolic extract of Glycosmis arborea facilitated wound healing significantly, corroborating the folk medicinal use of this plant.
Department of Pharmacology, SRM College of Pharmacy, SRM University, Kattankulathur 603203, India. janaki.srmcp@gmail.com
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Silambujanaki
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Bala Tejo Chandra
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Anil Kumar
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Chitra
D000818:Animals; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*; D051381:Rats; D027821:Rutaceae / Q000737:chemistry*; D014945:Wound Healing / Q000187:drug effects*
D010936:Plant Extracts