Rapid tagging and integration of genes in Giardia intestinalis.
We developed a series of plasmids that allow C-terminal tagging of any gene in its endogenous locus in Giardia intestinalis, with different epitope tags (triple hemagglutinin [3HA] and triple Myc [3Myc]) and selection markers (puromycin, neomycin, and a newly developed marker, blasticidin). Using these vectors, cyclin B and aurora kinase were tagged, expressed, and localized.
Department of Molecular and Cell Biology, University of California, Berkeley, 345 Life Sciences Addition, Berkeley, CA 94720-3200, USA.
Stéphane
S
Gourguechon
W Zacheus
WZ
Cande
D064127:Aurora Kinases; D019926:Cyclin B / Q000235:genetics / Q000378:metabolism; D055879:Gene Knock-In Techniques; D017125:Genes, Protozoan; D005818:Genetic Engineering; D005819:Genetic Markers; D005822:Genetic Vectors; D016829:Giardia lamblia / Q000235:genetics* / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism
D019926:Cyclin B; D005819:Genetic Markers; D064127:Aurora Kinases; D017346:Protein Serine-Threonine Kinases