[Beta-arrestin and NF-kappaB, AP-1 activity in peripheral blood mononuclear cells of guinea pigs sensitized by trichloroethylene].
OBJECTIVE
To explore the regulatory mechanism of immune response of guinea pigs sensitized by trichloroethylene (TCE), and the expression level of 3-arrestin, and the activity of NF-kappaB and AP-1 in peripheral blood mononuclear cells (PBMC) of guinea pigs sensitized by TCE.


METHODS
Guinea pigs were treated with TCE based on the guinea pig maximum response test (GPMT); Blank control group and DNCB positive control group were established. Scores of skin reaction were evaluated and used to determine whether or not allergy in guinea pig. Then TCE treated group was divided into sensitized group or un-sensitized group. The expression levels of beta-arrestin protein, activity of NF-kappaB and AP-1 in PBMC were detected by Western Blotting and EMSA, respectively. TNF-alpha level in serum was detected by ELISA kits.


RESULTS
No erythema or edema was found in the control group; part of guinea pigs treated with TCE developed erythema and edema, while obvious erythema and edema could be found in DNCB group. The sensitization rates were 71.4% and 100% in TCE and DNCB group, respectively. Compared with TCE un-sensitized group, expression of beta-arrestin and AP-1 activity were not significantly different in TCE sensitized group (P > 0.05). While the NF-kappaB activity was elevated obviously (P < 0.05). Compared with blank control groups [(32.118 +/- 12.550) pg/ml], serum TNF-alpha levels in TCE sensitized groups [(55.485 +/- 8.732) pg/ml] significantly elevated (P < 0.05);


CONCLUSIONS
In guinea pigs, beta-arrestin and AP-1 may not be activated, while the NF-kappaB activation is significant, and plays a immune regulatory role in the immune reaction of allergy induced by TCE.
Department of Occupational and Environmental Health, School of Public Health, Anhui Medical University, Hefei 230032, China.
Li-jie
LJ
Wang
Rui-juan
RJ
Guo
Tong
T
Shen
Qi-xing
QX
Zhu
D000818:Animals; D019390:Arrestins / Q000097:blood*; D004487:Edema / Q000139:chemically induced; D004890:Erythema / Q000139:chemically induced; D005260:Female; D006168:Guinea Pigs; D006967:Hypersensitivity / Q000097:blood*; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D016328:NF-kappa B / Q000097:blood*; D018808:Transcription Factor AP-1 / Q000097:blood*; D014241:Trichloroethylene / Q000633:toxicity*; D014409:Tumor Necrosis Factor-alpha / Q000097:blood; D000071557:beta-Arrestins
D019390:Arrestins; D016328:NF-kappa B; D018808:Transcription Factor AP-1; D014409:Tumor Necrosis Factor-alpha; D000071557:beta-Arrestins; D014241:Trichloroethylene