Cross-reactivity of anti-Eimeria tenella antibody fragments on merozoites and sporozoites of different chicken Eimeria species.
Eimeria tenella-specific antibodies were examined for the cross-reactivity on the sporozoites and merozoites of E. tenella, Eimeria maxima, Eimeria acervulina and Eimeria brunetti in an indirect fluorescence antibody test. Two of nine antibodies showed cross-reactivity with sporozoites of E. maxima, E. acervulina and E. brunetti; however, the localization of specific fluorescence differed between species. No antibody binding was observed on merozoites. The suitability of these antibodies to alter the infectivity of Eimeria sporozoites and/or merozoites must be verified in cell culture models and in vivo experimental infections.
Institute of Parasitology, Faculty of Veterinary Medicine, University Leipzig, Leipzig, Germany. redabast@hotmail.de
Reda E
RE
Khalafalla
Arwid
A
Daugschies
Viktor
V
Dyachenko
D000818:Animals; D000913:Antibodies, Protozoan / Q000276:immunology*; D000918:Antibody Specificity; D000953:Antigens, Protozoan / Q000276:immunology; D002645:Chickens; D003048:Coccidiosis / Q000469:parasitology / Q000662:veterinary; D056738:Cross Protection; D003429:Cross Reactions; D016786:Eimeria tenella / Q000276:immunology*; D019084:Fluorescent Antibody Technique, Indirect; D052940:Merozoites / Q000276:immunology*; D018532:Pisum sativum; D046549:Plantibodies / Q000276:immunology; D030821:Plants, Genetically Modified; D011201:Poultry Diseases / Q000469:parasitology; D011994:Recombinant Proteins / Q000276:immunology; D013045:Species Specificity; D034101:Sporozoites / Q000276:immunology*
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D046549:Plantibodies; D011994:Recombinant Proteins