In vitro antioxidant properties, DNA damage protective activity, and xanthine oxidase inhibitory effect of cajaninstilbene acid, a stilbene compound derived from pigeon pea [Cajanus cajan (L.) Millsp.] leaves.
The antioxidant properties, DNA damage protective activities, and xanthine oxidase (XOD) inhibitory effect of cajaninstilbene acid (CSA) derived from pigeon pea leaves were studied in the present work. Compared with resveratrol, CSA showed stronger antioxidant properties, DNA damage protective activity, and XOD inhibition activity. The IC(50) values of CSA for superoxide radical scavenging, hydroxyl radical scavenging, nitric oxide scavenging, reducing power, lipid peroxidation, and XOD inhibition were 19.03, 6.36, 39.65, 20.41, 20.58, and 3.62 μM, respectively. CSA possessed good protective activity from oxidative DNA damage. Furthermore, molecular docking indicated that CSA was more potent than resveratrol or allopurinol to interact with the active site of XOD (calculated free binding energy: -229.71 kcal mol(-1)). On the basis of the results, we conclude that CSA represents a valuable natural antioxidant source and may potentially be applicable in health food industry.
Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin, China.
Nan
N
Wu
Yu
Y
Kong
Yujie
Y
Fu
Yuangang
Y
Zu
Zhiwei
Z
Yang
Mei
M
Yang
Xiao
X
Peng
Thomas
T
Efferth
D016587:Antimutagenic Agents / Q000032:analysis*; D000975:Antioxidants / Q000032:analysis*; D036561:Cajanus / Q000737:chemistry*; D004249:DNA Damage; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004791:Enzyme Inhibitors / Q000032:analysis*; D006801:Humans; D010936:Plant Extracts / Q000032:analysis*; D018515:Plant Leaves / Q000737:chemistry; D012459:Salicylates / Q000032:analysis*; D013267:Stilbenes / Q000032:analysis*; D014969:Xanthine Oxidase / Q000037:antagonists & inhibitors*
C549543:3-hydroxy-4-prenyl-5-methoxystilbene-2-carboxylic acid; D016587:Antimutagenic Agents; D000975:Antioxidants; D004269:DNA, Bacterial; D004791:Enzyme Inhibitors; D010936:Plant Extracts; D012459:Salicylates; D013267:Stilbenes; D014969:Xanthine Oxidase