Canonical protein splicing of a class 1 intein that has a class 3 noncanonical sequence motif.
A Thermobifida fusca intein has two characteristics of class 3 inteins: a noncontiguous covariant Trp-Cys-Thr triplet and a Ser flanking its C terminus. However, it has Cys at position one, characteristic of class 1 inteins. Splicing does not require the internal Cys, which may instead coordinate the active site. Therefore, the intein is class 1.
Department of Chemistry, College of the Holy Cross, Worcester, MA 01610, USA.
Julie N
JN
Reitter
Kenneth V
KV
Mills
D000192:Actinomycetales / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020816:Amino Acid Motifs; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D047668:Inteins; D019154:Protein Splicing
D001426:Bacterial Proteins