Merely two mutations switch a DNA-hydrolyzing deoxyribozyme from heterobimetallic (Zn2+/Mn2+) to monometallic (Zn2+-only) behavior.
A deoxyribozyme that hydrolyzes DNA phosphodiester linkages with a requirement for both Zn(2+) and Mn(2+) is switched by only two nucleotide mutations to require Zn(2+) alone, demonstrating that DNA-catalyzed DNA hydrolysis can be achieved using only one metal ion cofactor.
Department of Chemistry, University of Illinois at Urbana-Champaign, 600 South Mathews Avenue, Urbana, IL 61801, USA.
Ying
Y
Xiao
Emily C
EC
Allen
Scott K
SK
Silverman
D001483:Base Sequence; D002413:Cations, Divalent / Q000737:chemistry; D004247:DNA / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D021881:DNA, Catalytic / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D004952:Esters / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008345:Manganese / Q000737:chemistry*; D010756:Phosphoric Acids / Q000737:chemistry; D017354:Point Mutation; D013997:Time Factors; D015032:Zinc / Q000737:chemistry*
D002413:Cations, Divalent; D021881:DNA, Catalytic; D004952:Esters; D010756:Phosphoric Acids; D008345:Manganese; D004247:DNA; D015032:Zinc