Chemokine (CCR) and fractalkine (CX3CR) receptors and end stage renal disease.
OBJECTIVE
Genetic polymorphisms of chemokines and their receptors were reported to be independent risk factors for inflammation associated disease. We explored the role of CCR5-Δ32, CCR5-G59029A, CX3CR1 V249I and T280M gene polymorphisms as susceptibility for end stage renal disease (ESRD).


METHODS
We genotyped 258 ESRD and 569 healthy controls by sequence-specific primers and RFLP and examined their association.


RESULTS
There was significant difference in genotype frequencies of CCR5-G59029A (p = 0.005), and CX3CR1 V249I (p < 0.0001) between ESRD and controls. No homozygous individuals were observed for CCR5-Δ32. The haplotype analysis of all four studied genes reveled that haplotype +/A/T/I was more significant in patients and associated with higher risk (OR = 2.95) of ESRD. Further, the haplotype of CX3CR1 (T280M, V249I) gene showed 3.6-fold higher in an individual carrying T/I haplotype. No risk was seen for CCR5 haplotypes.


CONCLUSIONS
These results highlight the role of CCR5 and CX3CR1 in ESRD.
Department of Medical Genetics, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Raebareli Road, Lucknow, UP, 226014, India.
Minal
M
Borkar
Gaurav
G
Tripathi
Raj Kumar
RK
Sharma
Satya Narayan
SN
Sankhwar
Suraksha
S
Agrawal
D000818:Animals; D000073883:CX3C Chemokine Receptor 1; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D007676:Kidney Failure, Chronic / Q000235:genetics* / Q000276:immunology*; D008297:Male; D011110:Polymorphism, Genetic; D019713:Receptors, CCR5 / Q000235:genetics*; D018121:Receptors, Cytokine / Q000235:genetics*; D015728:Receptors, HIV / Q000235:genetics*
D000073883:CX3C Chemokine Receptor 1; D019713:Receptors, CCR5; D018121:Receptors, Cytokine; D015728:Receptors, HIV