Biological screening of araripe basin medicinal plants using Artemia salina Leach and pathogenic bacteria.
BACKGROUND
Many medicinal plant species from the Araripe Basin are widely known and used in folk medicine and for commercial manufacturing of phytotherapeutic products. Few ethnobotanical and pharmacological studies have been undertaken in this region, however, in spite of the great cultural and biological diversity found there.


METHODS
Extracts of 11 plant species collected from Ceará state, Brazil, were subjected to the brine shrimp lethality test in order to detect potential sources of novel cytotoxic, antitumor compounds. The larvicidal activity, based on the percentage of larval mortality, was evaluated after 24 h exposure to the treatments.


RESULTS
All species tested showed good larvicidal activity as compared to a reference compound and literature data. The extract from Vanillosmopsis arborea was the most active with an LC(50) of 3.9 μg/ml. Best results were shown by Lantana montevidensis against Pseudomonas aeruginosa [minimum inhibitory concentration (MIC) 8μg/ml] and Escherichia coli (MIC 32 μg/ml), Zanthoxylum rhoifolium against E. coli (MIC, 256 μg/ml) and Staphylococcus aureus (MIC 64 μg/ml) and Croton zenhtneri against S. aureus (MIC 64 μg/ml).


CONCLUSIONS
Chemical tests indicated that a wide variety of natural product classes was present in those extracts that showed significant activities in the bioassays.
Programa de Pós-Graduação em Bioprospecção Molecular, Departamento de Química Biológica, Laboratório de Pesquisa de Produtos Naturais, Universidade Regional do Cariri, Rua Cel. Antônio Luiz 1161, Pimenta, 63105-000 Crato-CE, Brasil.
José Galberto M
JG
da Costa
Adriana R
AR
Campos
Samara A
SA
Brito
Carla Karine B
CK
Pereira
Erlânio O
EO
Souza
Fabíola Fernandes G
FF
Rodrigues