Byakkokaninjinto prevents body water loss by increasing the expression of kidney aquaporin-2 and skin aquaporin-3 in KKAy mice.
Byakkokaninjinto (BKN) is an herbal medicine used for the relief of diuresis, thirst and dermal pruritus that are associated with diabetes. The effects of BKN on the expression of aquaporins (AQPs) in the kidney, salivary gland and skin were investigated in order to clarify the mechanism of drug action. Seven-week-old KKAy mice were given feed containing 4.5% BKN for 4 weeks. Compared with the control group, BKN administration did not affect the blood glucose and insulin concentration. However, water intake and urine volume were significantly reduced. AQP2 protein expression in the kidney inner medullary was significantly increased after BKN administration. AQP3 mRNA and protein expression in skin tissue was significantly increased after BKN administration. However, BKN administration did not affect AQP5 mRNA expression in the salivary gland. These results suggest that BKN treatment relieves diuresis, thirst, and dermal pruritis by increasing kidney AQP2 expression and skin AQP3 expression.
Department of Clinical Pharmacokinetics, Hoshi University, Tokyo, Japan.
Takashi
T
Aburada
Nobutomo
N
Ikarashi
Mai
M
Kagami
Yuhei
Y
Ichikawa
Megumi
M
Sugitani
Ayaka
A
Maniwa
Harumi
H
Ueda
Takahiro
T
Toda
Kiyomi
K
Ito
Wataru
W
Ochiai
Ryo
R
Matsushita
Ken-ichi
K
Miyamoto
Kiyoshi
K
Sugiyama
D031403:Anemarrhena / Q000737:chemistry; D000818:Animals; D051399:Aquaporin 2 / Q000096:biosynthesis*; D051400:Aquaporin 3 / Q000096:biosynthesis*; D001834:Body Water / Q000378:metabolism*; D003924:Diabetes Mellitus, Type 2 / Q000188:drug therapy / Q000378:metabolism; D004231:Diuresis / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D006035:Glycyrrhiza / Q000737:chemistry; D006801:Humans; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D023421:Models, Animal; D012275:Oryza / Q000737:chemistry; D005894:Panax / Q000737:chemistry; D010936:Plant Extracts / Q000494:pharmacology*; D012469:Salivary Glands / Q000378:metabolism; D012867:Skin / Q000187:drug effects* / Q000378:metabolism
D051399:Aquaporin 2; D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; C108060:byakko-ka-ninjin-to; D051400:Aquaporin 3