Toll-like receptors and cytokines/cytokine receptors polymorphisms associate with non-response to hepatitis B vaccine.
It is well documented that 5-10% hepatitis B adult vaccinees are non- and hypo-responders and probably are not adequately protected against hepatitis B virus (HBV) infection. The sequence variations of genes involved in processes such as pathogen recognition, antigen processing and presentation, and differentiation/maturation of lymphocytes may affect the duration and intensity of protective humoral immune response to the hepatitis B vaccine. In this study, frequencies of 53 known SNPs within 21 candidate genes were analyzed among 46 responders and 24 non-responders. Four SNPs (rs2243248, rs1805015, rs1295686 and rs3804100) in IL-4, IL-4RA, IL-13 and TLR2 genes were found significantly associated with the vaccinees' status of serum anti-HBV response triggered by the vaccine (P<0.05). Two SNPs (rs1295686 and rs1805015) also showed significant association with the vaccine-induced immune response when analyzed together with risk factors such as age and gender, by multivariable logistic regression analysis (P<0.05). Further, haplotype analysis showed that the AG haplotype defined by SNPs rs1143633 (IL-1B; intron) and rs1143627 (IL-1B; intron) was present more frequently in non-responders than in responders (P=0.035). Thus, specific SNPs in genes of cytokines/cytokine receptors and TLR2 were associated with status of the hepatitis B vaccine-induced protective humoral immune response.
Department of Microbiology, Peking University Health Science Center, Beijing 100191, China.
Jie
J
Chen
Zhenglun
Z
Liang
Fengmin
F
Lu
Xin
X
Fang
Shuang
S
Liu
Ying
Y
Zeng
Fengcai
F
Zhu
Xiangmei
X
Chen
Tao
T
Shen
Jie
J
Li
Hui
H
Zhuang
D016207:Cytokines / Q000235:genetics / Q000276:immunology*; D005260:Female; D006510:Hepatitis B Antibodies / Q000097:blood; D017325:Hepatitis B Vaccines / Q000276:immunology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D018121:Receptors, Cytokine / Q000235:genetics / Q000276:immunology*; D051193:Toll-Like Receptors / Q000235:genetics / Q000276:immunology*
D016207:Cytokines; D006510:Hepatitis B Antibodies; D017325:Hepatitis B Vaccines; D018121:Receptors, Cytokine; D051193:Toll-Like Receptors