Effect of acetaminophen, ibuprofen and methylprednisolone on different parameters of human osteoblast-like cells.
Anti-inflammatories are divided between steroidal and non-steroidal drugs according to their chemical nature. They are frequently used in combination to achieve analgesia and reduce inflammation after surgery, especially when bone tissue is affected. However, their effects on bone level are not well known, and results of the scant studies in humans have been contradictory.


OBJECTIVE
To study the short-term effect of ibuprofen, acetaminophen and methylprednisolone at therapeutic doses on different cell parameters of human osteoblasts in culture.


METHODS
Osteoblasts were derived from samples of human bone sections obtained during third molar surgery. Cell lines were incubated in culture medium with different concentrations of anti-inflammatories (5 or 25 μM of ibuprofen or acetaminophen, 10(-6) or 10(-8)M of methylprednisolone) for 24 or 48h. Flow cytometry was used to study proliferation, antigenic profile, phagocytic activity and cell cycle.


RESULTS
All three anti-inflammatories inhibited osteoblastic proliferation, but no significant cell cycle changes were observed. The drugs had no effect on antigenic profile or phagocytic capacity.


CONCLUSIONS
These results suggest that ibuprofen, acetaminophen and methylprednisolone at therapeutic doses reduce osteoblast growth without affecting other cell parameters, such as antigenic profile or cell cycle. Use of these drugs should be reconsidered in clinical situations that require a rapid healing of bone defects.
School of Health Sciences, University of Granada, Spain. ogm@ugr.es
Olga
O
García-Martínez
Lourdes
L
Díaz-Rodríguez
Laura
L
Rodríguez-Pérez
Elvira
E
De Luna-Bertos
Candela
C
Reyes Botella
C Concepción
CC
Ruiz
D000082:Acetaminophen / Q000494:pharmacology*; D000893:Anti-Inflammatory Agents / Q000145:classification / Q000494:pharmacology*; D001861:Bone Regeneration / Q000187:drug effects; D016723:Bone Remodeling / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D006801:Humans; D007052:Ibuprofen / Q000494:pharmacology*; D008334:Mandible / Q000166:cytology; D008775:Methylprednisolone / Q000494:pharmacology*; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects*; D055815:Young Adult
D000893:Anti-Inflammatory Agents; D000082:Acetaminophen; D007052:Ibuprofen; D008775:Methylprednisolone