Molecular characterization of rare G12P[6] rotavirus isolates closely related to G12 strains from the United States, CAU 195 and CAU 214.
Two human G12 rotaviruses, CAU 195 and CAU 214, were isolated from South Korea using cell culture and characterized on the basis of sequence divergence in the VP7, VP4, and NSP4 genes. Phylogenetic analysis of the VP7 gene sequences indicated that these strains clustered into lineage III and were most closely related to G12 rotaviruses isolated in the United States. The VP4 and NSP4 gene sequences showed that two strains belonged to the P[6]-Ia lineage and genotype [B]. This finding provides information that can be used to evaluate G12 strains and aid in the development of effective vaccines in the future.
Department of Microbiology, College of Medicine, Chung-Ang University, Seoul, South Korea.
Van Phan
VP
Le
Jong Bum
JB
Kim
Dong Hwa
DH
Shon
In Sik
IS
Chung
Yoosik
Y
Yoon
Kijeong
K
Kim
Sang-In
SI
Chung
Inseok
I
Lim
Wonyong
W
Kim
D016000:Cluster Analysis; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics*; D056910:Republic of Korea; D012401:Rotavirus / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000821:virology*; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014776:Virus Cultivation
D012367:RNA, Viral