Cellulase-producing bacteria from Thai higher termites, Microcerotermes sp.: enzymatic activities and ionic liquid tolerance.
The three highest hydrolysis-capacity-value isolates of Bacillus subtilis (A 002, M 015, and F 018) obtained from Thai higher termites, Microcerotermes sp., under different isolation conditions (aerobic, anaerobic, and anaerobic/aerobic) were tested for cellulase activities--FPase, endoglucanase, and β-glucosidase--at 37 °C and pH 7.2 for 24 h. Their tolerance to an ionic liquid, 1-butyl-3-methylimidazolium chloride ([BMIM]Cl), was also investigated. The results showed that the isolate M 015 provided the highest endoglucanase activity whereas the highest FPase and β-glucosidase activities were observed for the isolate F 018. The isolate F 018 also showed the highest tolerance to [BMIM]Cl in the range of 0.1-1.0 vol.%. In contrast, the isolate A 002 exhibited growth retardation in the presence of 0.5-1.0 vol.% [BMIM]Cl.
The Petroleum and Petrochemical College, Chulalongkorn University, Bangkok 10330, Thailand.
Kitipong
K
Taechapoempol
Thammanoon
T
Sreethawong
Pramoch
P
Rangsunvigit
Weerachart
W
Namprohm
Bandhit
B
Thamprajamchit
Sirirat
S
Rengpipat
Sumaeth
S
Chavadej
D000222:Adaptation, Physiological / Q000187:drug effects*; D000818:Animals; D001419:Bacteria / Q000187:drug effects* / Q000201:enzymology* / Q000254:growth & development / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000096:biosynthesis; D002480:Cellulase / Q000096:biosynthesis*; D007093:Imidazoles / Q000633:toxicity*; D052578:Ionic Liquids / Q000633:toxicity*; D020049:Isoptera / Q000382:microbiology*; D013696:Temperature; D013785:Thailand
D001426:Bacterial Proteins; D007093:Imidazoles; D052578:Ionic Liquids; C502841:1-butyl-3-methylimidazolium chloride; D002480:Cellulase