DNA adducts of ortho-toluidine in human bladder.
BACKGROUND
4-Aminobiphenyl (4-ABP) and o-toluidine are known human bladder carcinogens, but only 4-ABP-releasing DNA adducts are known.


METHODS
Determination of 4-ABP and o-toluidine-releasing DNA adducts in epithelial and submucosal bladder tissues of sudden death victims (SDV: n=46), and bladder tumours (n=12) by gas chromatography/mass spectrometry.


RESULTS
Above background, 4 and 11 of 12 tumour samples contained adducts of 4-ABP (0.057 ± 0.125 fmol/µg DNA) and o-toluidine (8.72 ± 4.49 fmol/µg DNA), respectively. Lower adduct levels were present in both epithelial and submucosal bladder tissues of SDV (4-ABP: 0.011 ± 0.022 and 0.019 ± 0.047 fmol/µg DNA; o-toluidine: 0.24 ± 0.63 and 0.27 ± 0.70 fmol/µg DNA).


CONCLUSIONS
Detection of o-toluidine-releasing DNA adducts support the carcinogenicity of o-toluidine in the human bladder.
Walther Straub Institute, Department of Toxicology, Ludwig-Maximilians-University, Nussbaumstrasse 26, D-80336 Munich, Germany.
Francine
F
Böhm
Dagmar
D
Schmid
Stefan
S
Denzinger
Wolf F
WF
Wieland
Elmar
E
Richter
D000611:Aminobiphenyl Compounds / Q000032:analysis; D001344:Autopsy; D001706:Biopsy; D002273:Carcinogens / Q000032:analysis; D002849:Chromatography, Gas; D004247:DNA / Q000378:metabolism; D018736:DNA Adducts / Q000032:analysis*; D003645:Death, Sudden; D004847:Epithelial Cells / Q000737:chemistry* / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D014052:Toluidines / Q000032:analysis*; D001743:Urinary Bladder / Q000737:chemistry* / Q000378:metabolism; D001749:Urinary Bladder Neoplasms / Q000737:chemistry* / Q000378:metabolism / Q000503:physiopathology
D000611:Aminobiphenyl Compounds; D002273:Carcinogens; D018736:DNA Adducts; D014052:Toluidines; C006757:4-biphenylamine; D004247:DNA; C023622:2-toluidine