Interaction of Allium sativum leaf agglutinin with midgut brush border membrane vesicles proteins and its stability in Helicoverpa armigera.
Allium sativum leaf agglutinin (ASAL) binds to several proteins in the midgut of Helicoverpa armigera and causes toxicity. Most of these were glycosylated. Six ASAL-binding proteins were selected for identification. PMF and MS/MS data showed their similarity with midgut aminopeptidase APN2, polycalins and alkaline phosphatase of H. armigera, cadherin-N protein (partial AGAP009726-PA) of Acyrthosiphon pisum, cytochrome P450 (CYP315A1) of Manduca sexta and alkaline phosphatase of Heliothis virescens. Some of the ASAL-binding midgut proteins were similar to the larval receptors responsible for the binding of δ-endotoxin proteins of Bacillus thuringiensis. Galanthus nivalis agglutinin also interacted with most of the ASAL-binding proteins. The ASAL showed resistance to midgut proteases and was detected in the larval hemolymph and excreta. Immunohistochemical staining revealed the presence of ASAL in the body tissue also.
National Botanical Research Institute, Council of Scientific and Industrial Research, Rana Pratap Marg, Lucknow, Uttar Pradesh, India.
Santosh Kumar
SK
Upadhyay
Manisha
M
Mishra
Harpal
H
Singh
Amol
A
Ranjan
Krishnappa
K
Chandrashekar
Praveen Chandra
PC
Verma
Pradhyumna Kumar
PK
Singh
Rakesh
R
Tuli
D000818:Animals; D004064:Digestive System / Q000737:chemistry* / Q000378:metabolism; D019476:Insect Proteins / Q000737:chemistry* / Q000378:metabolism; D037241:Mannose-Binding Lectins / Q000737:chemistry*; D008562:Membrane Glycoproteins / Q000737:chemistry* / Q000378:metabolism; D008871:Microvilli / Q000737:chemistry / Q000378:metabolism; D009036:Moths / Q000378:metabolism*; D010940:Plant Proteins / Q000737:chemistry*; D011485:Protein Binding; D055550:Protein Stability; D053719:Tandem Mass Spectrometry
C422947:ASAI protein, Allium sativum; D019476:Insect Proteins; D037241:Mannose-Binding Lectins; D008562:Membrane Glycoproteins; D010940:Plant Proteins