Early eradication of persistent Salmonella infection primes antibody-mediated protective immunity to recurrent infection.
Typhoid fever is a systemic, persistent infection caused by host-specific strains of Salmonella. Although the use of antibiotics has reduced the complications associated with primary infection, recurrent infection remains an important cause of ongoing human morbidity and mortality. Herein, we investigated the impacts of antibiotic eradication of primary infection on protection against secondary recurrent infection. Using a murine model of persistent Salmonella infection, we demonstrate protection against recurrent infection is sustained despite early eradication of primary infection. In this model, protection is not mediated by CD4(+) or CD8(+) T cells because depletion of these cells either alone or in combination prior to rechallenge does not abrogate protection. Instead, infection followed by antibiotic-mediated clearance primes robust levels of Salmonella-specific antibody that can adoptively transfer protection to naïve mice. Thus, eradication of persistent Salmonella infection primes antibody-mediated protective immunity to recurrent infection.
Department of Pediatrics, University of Minnesota School of Medicine, Center for Infectious Disease and Microbiology Translational Research, USA.
Tanner M
TM
Johanns
Calvin Y
CY
Law
Lokeshchandra A
LA
Kalekar
Hope
H
O'Donnell
James M
JM
Ertelt
Jared H
JH
Rowe
Sing Sing
SS
Way
D000818:Animals; D000900:Anti-Bacterial Agents / Q000627:therapeutic use*; D000907:Antibodies, Bacterial / Q000097:blood; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D000077422:Enrofloxacin; D005260:Female; D024841:Fluoroquinolones / Q000627:therapeutic use*; D056724:Immunity, Humoral; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D012475:Salmonella / Q000276:immunology; D012480:Salmonella Infections / Q000188:drug therapy* / Q000276:immunology* / Q000401:mortality; D013997:Time Factors
D000900:Anti-Bacterial Agents; D000907:Antibodies, Bacterial; D024841:Fluoroquinolones; D000077422:Enrofloxacin