Expression analysis of five maize MAP kinase genes in response to various abiotic stresses and signal molecules.
Mitogen-activated protein kinase (MAPK) cascades are universal signal transduction modules in eukaryotes. Plant MAPK cascades are complicated networks and play vital roles in signal transduction induced by biotic and abiotic stresses. In this paper, expression patterns of MAPKs in maize roots treated with low-temperature, osmotic stresses, wounding, plant hormones and UV-C irradiation were investigated. Semi-quantitative RT-PCR reveals that the expression of MAPKs in maize roots which treated with low-temperature in light or in low light are inducible. The expression patterns of MAPKs in maize roots with treatments of CaCl2, SA, GA and wounding are approximately the same. A detailed time course experiment shows that the expression patterns of ZmSIMK are different with treatments of PEG and NaCl, respectively. These results suggest that the expression patterns of MAPKs are complicated and the signal pathways are interlaced into a network in maize roots.
State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, 61 Daizong street, Taian, 271018, Shandong, People's Republic of China.
Tao
T
Wu
Xiang-Pei
XP
Kong
Xiao-Juan
XJ
Zong
Da-Peng
DP
Li
De-Quan
DQ
Li
D000595:Amino Acid Sequence; D032461:Chromosomes, Plant / Q000235:genetics; D004789:Enzyme Activation / Q000187:drug effects / Q000528:radiation effects; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects / Q000528:radiation effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000528:radiation effects; D017343:Genes, Plant / Q000235:genetics*; D020928:Mitogen-Activated Protein Kinases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D009997:Osmotic Pressure / Q000187:drug effects / Q000528:radiation effects; D010802:Phylogeny; D010937:Plant Growth Regulators / Q000494:pharmacology; D018517:Plant Roots / Q000187:drug effects / Q000201:enzymology / Q000235:genetics; D011092:Polyethylene Glycols / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects; D012965:Sodium Chloride / Q000494:pharmacology; D013312:Stress, Physiological / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects; D013696:Temperature; D014466:Ultraviolet Rays; D003313:Zea mays / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics* / Q000528:radiation effects
D010937:Plant Growth Regulators; D012333:RNA, Messenger; D011092:Polyethylene Glycols; D012965:Sodium Chloride; D020928:Mitogen-Activated Protein Kinases