[Evaluation of recombinant 29,000 extra membranous protein for the immunodiagnosis of schistosomiasis japonica].
OBJECTIVE
To evaluate the recombinant membranous protein 29000 (rSj29) of Schistosoma japonicum in the immunodiagnosis of schistosomiasis by indirect ELISA.


METHODS
394 serum samples collected from two low-endemic villages in Anhui Province were tested by rSj29-ELISA and AWA-ELISA using adult worm antigen (AWA) of S. japonicum. Meanwhile all subjects were each tested by 9 examinations with 3 fecal samples by modified Kato-Katz method, and by indirect hemagglutination assay (IHA).


RESULTS
It was found that the positive detection rates of stool examination, IHA, rSj29-ELISA and AWA-ELISA were 4.8% (19/394), 62.2% (245/394), 68.3% (269/394), and 89.9% (354/394), respectively. There was no statistical difference between rSj29-ELISA and IHA (chi2 = 3.2, P > 0.05) with the coincidence of 80.7% (318/394). The coincidence of IHA, rSj29-ELISA and AWA-ELISA with stool examination was 42.6% (168/394), 36.0% (142/394), and 15.0% (59/394), respectively.


CONCLUSIONS
The indirect ELISA with rSj29 antigen shows similar effect as IHA for the diagnosis of schistosomiasis.
Department of Microbiology and Parasitology, Anhui Medical University, Hefei 230032, China.
Ping
P
Wang
Cui-ping
CP
Ren
Tian-ping
TP
Wang
Ji-jia
JJ
Shen
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006386:Hemagglutination Tests; D006801:Humans; D008297:Male; D008875:Middle Aged; D011994:Recombinant Proteins; D012549:Schistosoma japonicum / Q000276:immunology*; D012554:Schistosomiasis japonica / Q000175:diagnosis* / Q000276:immunology*; D055815:Young Adult
D011994:Recombinant Proteins