Systematic phytochemical investigation of Abies spectabilis.
Systematical phytochemical investigations on Abies spectabilis afforded 72 chemical constituents. On the basis of physical and spectroscopic data, including 1D and 2D homo- and heteronuclear NMR experiments (heteronuclear single quantum coherence (HSQC), (1)H-(1)H correlation spectroscopy (COSY), heteronuclear multiple bond connectivity (HMBC), and nuclear Overhauser effect spectroscopy (NOESY)), and by comparison with the literature references, they were identified as 3 triterpenoids, 23 diterpenoids, 1 sesquiterpenoid, 13 flavonoids, 12 lignans, and 20 other components. Among these compounds, three were identified as new including abieta-7,13-diene-12α-methoxy-18-oic acid (1), 7α-methoxy-dehydroabietic acid (2), and 5-hydroxy-6-methyl-7,4'-dimethoxyflavone-8-O-β-D-glucopyranoside (3). These three new compounds (1-3) and all the known terpenoids (4-28) were tested for cytotoxic activities against four tumor cell lines: A549, COLO-25, QGY-25, and THP-1. However, none of them showed a positive effect (IC₅₀>100 µM).
Department of Natural Product Chemistry, School of Pharmacy, Second Military Medical University, Shanghai, China.
Liang
L
Wu
Yong-Li
YL
Li
Su-Mei
SM
Li
Xian-Wen
XW
Yang
Jia-Han
JH
Xia
Le
L
Zhou
Wei-Dong
WD
Zhang
D028202:Abies / Q000737:chemistry*; D045784:Abietanes / Q000737:chemistry* / Q000302:isolation & purification / Q000633:toxicity; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000633:toxicity; D045744:Cell Line, Tumor; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000633:toxicity; D005419:Flavonoids / Q000737:chemistry* / Q000302:isolation & purification / Q000633:toxicity; D005960:Glucosides / Q000737:chemistry* / Q000302:isolation & purification / Q000633:toxicity; D006801:Humans; D017705:Lignans / Q000737:chemistry / Q000302:isolation & purification / Q000633:toxicity; D009682:Magnetic Resonance Spectroscopy; D008968:Molecular Conformation; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000633:toxicity
C556734:5-hydroxy-6-methyl-7,4'-dimethoxyflavone-8-O-beta-D-glucopyranoside; C556735:7alpha-methoxy-dehydroabietic acid; D045784:Abietanes; D000970:Antineoplastic Agents; D004224:Diterpenes; D005419:Flavonoids; D005960:Glucosides; D017705:Lignans; D014315:Triterpenes; C556737:abieta-7,13-diene-12alpha-methoxy-18-oic acid