Usefulness of powdered and fresh egg yolk for cryopreservation of Zebu bull spermatozoa.
This experiment was designed to compare powdered egg yolk with fresh egg yolk in an extender for cryopreservation of Zebu bull semen. Sperm motility, plasma membrane integrity and viability were assessed at different stages of cryopreservation (post-dilution, pre-freezing and post-thawing). Sperm plasma membrane integrity remained similar at all the stages of cryopreservation. Sperm motility and viability were significantly higher after thawing in the extender containing powdered egg yolk. In conclusion, powdered egg yolk may be used in an extender for the cryopreservation of Zebu bull spermatozoa.
Animal Physiology Laboratory, Department of Zoology, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan.
Muhammad S
MS
Ansari
Bushra A
BA
Rakha
Syed M H
SM
Andrabi
Shamim
S
Akhter
D000818:Animals; D002417:Cattle; D002470:Cell Survival; D015925:Cryopreservation; D004530:Egg Yolk; D008297:Male; D013081:Sperm Motility; D013094:Spermatozoa