[Roles of autocrine soluble fibronectin on the maintenance of cell shape of osteosarcoma MG-63 cells].
OBJECTIVE
To investigate the roles of autocrine soluble FN of MG-63 cells in cell shape maintaining.


METHODS
Human osteosarcoma MG-63 cells (1 × 10(4); cells/cm(2);) were routinely cultured in MEM medium+10% fetal bovine serum (FBS) for 24 h and then the medium was replaced by fresh medium(the group without autocrine soluble FN), conditional medium of collected after 24 h of MG-63 cells (the group containing autocrine soluble FN ) and fresh medium with 20 microg/mL FN( the group with plasma FN) after 24 h, and the cell morphology was observed using phase contrast microscope; The concentrations of soluble FN in fresh and conditional medium were detected; Microfilament and insoluble FN changes of MG-63 cells were investigated.


RESULTS
The concentration of soluble FN in conditional medium is much higher than that in the fresh medium (P<0.01). Fresh medium will result in the cell shape changing from spindle to round for 0.5 h. The cell began to spread for 1 h, and the cell shape recovered for 2 h; Conditioned medium has no significant effects on cell shape. Addition of FN (20 mg/L) to fresh medium could inhibit the cell shape change induced by fresh medium. Microfilament and insoluble FN were disorganized by fresh medium.


CONCLUSIONS
These results indicated that autocrine soluble FN of osteosarcoma MG-63 cells may be involved in cell shape maintaining.
Institute of Special Enviromental Biophysics, Key Laboratory for Space Biosciences and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an 710072, China. royluo1980@mail.nwpu.edu.cn
Ming-Zhi
MZ
Luo
Jing-Bao
JB
Li
Sheng-Sheng
SS
Li
Jian-Ping
JP
Cao
Rui
R
Meng
Wei
W
Zhang
Peng
P
Shang
D008841:Actin Cytoskeleton / Q000378:metabolism; D000293:Adolescent; D045744:Cell Line, Tumor; D048430:Cell Shape; D005353:Fibronectins / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D008297:Male; D012516:Osteosarcoma / Q000378:metabolism* / Q000473:pathology*; D012995:Solubility
D005353:Fibronectins