Influence of novel nano-titanium implant surface on human osteoblast behavior and growth.
OBJECTIVE
The aim of the study is to investigate human osteoblast-like cell behavior and growth in the presence of 3 different titanium implant surfaces.


METHODS
Human stem cells were first obtained and then sorted by fluorescence-activated cell sorter from mesenchymal stem cell clusters of human dental papilla. The obtained human dental papilla stem cells were induced to differentiate into osteoblast-like cells and were then analyzed by reverse transcriptase polymerase chain reaction and Western blot analyses. The cells proliferated and were cultured onto 3 different titanium discs (sandblasted, sandblasted and large-grit acid-etched, and full contact coverage [FCC]) and analyzed by scanning electron microscope.


RESULTS
In all analyses samples, a high cell activity was observed, with typical osteoblast mature morphostructural response on rough surface. The high number of osteoblast-like cells was found on titanium FCC discs. At the same time, scanning electron microscope analysis confirmed the high biocompatibility of this surface.


CONCLUSIONS
The rapid maturation of the osteoblast-like cells on FCC titanium surface suggests that this structure could play a central role during initial phases of bone healing processes.
Department of Oral Science, University G. d'Annunzio, Chieti, Italy. tete@unich.it/filibertomastrangelo@hotmail.com
Stefano
S
Tetè
Filiberto
F
Mastrangelo
Raimondo
R
Quaresima
Raffaele
R
Vinci
Gilberto
G
Sammartino
Liborio
L
Stuppia
Enrico
E
Gherlone
D000293:Adolescent; D000469:Alkaline Phosphatase / Q000096:biosynthesis; D002448:Cell Adhesion; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible; D015921:Dental Implants; D003771:Dental Papilla / Q000166:cytology; D017267:Dental Prosthesis Design; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis; D005260:Female; D006023:Glycoproteins / Q000096:biosynthesis; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D049329:Nanostructures; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism; D015675:Osteocalcin / Q000096:biosynthesis; D010750:Phosphoproteins / Q000096:biosynthesis; D013499:Surface Properties; D014025:Titanium
D020099:Coated Materials, Biocompatible; D015921:Dental Implants; D016326:Extracellular Matrix Proteins; D006023:Glycoproteins; C486579:MEPE protein, human; D010750:Phosphoproteins; D015675:Osteocalcin; D014025:Titanium; D000469:Alkaline Phosphatase