Identification of major metabolites in rat urine and plasma of N(6) -(4-hydroxybenzyl) adenine riboside by LC/MS/MS.
N(6) -(4-hydroxybenzyl) adenine riboside, a novel neuroprotective compound found in Gastrodia elata at trace level, is regarded as a potential drug for the treatment of neural degenerative disease. To understand the metabolism of this compound, the metabolites in rat urine and plasma of N(6) -(4-hydroxybenzyl) adenine riboside were analyzed by HPLC-ESI-MS/MS after oral administration of this compound. Beside the parent compound, six phase I metabolites and four phase II metabolites in urine were detected by scanning all possible metabolites in extracted ion chromatograms mode. By comparing their product ion spectra and retention times with those of parent compound, these metabolites were identified and proved to be mainly formed via hydrolysis or hydroxylation in phase I, N-sulfation or N-glucuronidation in phase II or their combinations. Similarly, the parent compound, one phase I metabolite and two phase II metabolites were also identified in rat plasma. Therefore, the in vivo metabolic pathways of N(6) -(4-hydroxybenzyl) adenine riboside in rat were proposed.
Laboratory of Medicinal Chemistry, Dalian institute of Chemical Physics, Dalian, China.
Yujia
Y
Lei
Li
L
Wang
Mengchun
M
Cheng
Hongbin
H
Xiao
D000241:Adenosine / Q000031:analogs & derivatives* / Q000097:blood / Q000652:urine; D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000378:metabolism*; D031668:Gastrodia / Q000737:chemistry / Q000378:metabolism*; D005965:Glucuronates; D006900:Hydroxylation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013431:Sulfates; D053719:Tandem Mass Spectrometry / Q000379:methods*
D004365:Drugs, Chinese Herbal; D005965:Glucuronates; C519742:N6-(4-hydroxybenzyl)adenine riboside; D013431:Sulfates; D000241:Adenosine