Saucerneol G, a new lignan, from Saururus chinensis inhibits matrix metalloproteinase-9 induction via a nuclear factor κB and mitogen activated protein kinases in lipopolysaccharide-stimulated RAW264.7 cells.
This study was conducted to demonstrate the inhibitory effect of saucerneol G (SG), a new lignan, isolated from the aerial part of Saururus chinensis (Saururaceae) on lipopolysaccharide (LPS)-stimulated matrix metalloproteinase-9 (MMP)-9 inductions in RAW 264.7 cells. Aimed at evaluating the mechanism of action by which SG inhibits the LPS-mediated induction of MMP-9, the effects of SG on nuclear factor-κB (NF-κB) DNA binding activity, NF-κB-dependent reporter gene activity, inhibitory factor-κB (IκB) phosphorylation, degradation and p65 nuclear translocation were assessed. SG profoundly suppressed the DNA binding activity and the reporter gene activity as well as translocation of NF-κB p65 subunit. Furthermore, SG also dose dependently inhibited LPS-stimulated activation of mitogen-activated protein kinases (MAPKs). These findings suggest that SG may inhibit LPS-stimulated MMP-9 induction by blocking NF-κB and MAPKs activation.
Yeungnam University, Gyeongsan, Republic of Korea.
Yue
Y
Lu
Tae Gyun
TG
Hong
Meihua
M
Jin
Ju Hae
JH
Yang
Seok-Jong
SJ
Suh
Dong Gen
DG
Piao
Hyun-Kwon
HK
Ko
Chang-Seob
CS
Seo
Young-Chae
YC
Chang
Cheorl-Ho
CH
Kim
Jong-Keun
JK
Son
Hyeun Wook
HW
Chang
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D001692:Biological Transport / Q000187:drug effects; D004247:DNA / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D017930:Genes, Reporter / Q000502:physiology; D017705:Lignans / Q000302:isolation & purification / Q000494:pharmacology*; D008070:Lipopolysaccharides; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D051379:Mice; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D035261:Plant Components, Aerial; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018517:Plant Roots; D032134:Saururaceae / Q000737:chemistry*; D051996:Transcription Factor RelA / Q000378:metabolism*
D000893:Anti-Inflammatory Agents; D017705:Lignans; D008070:Lipopolysaccharides; D010936:Plant Extracts; D051996:Transcription Factor RelA; C559485:saucerneol G; D004247:DNA; D020928:Mitogen-Activated Protein Kinases; D020780:Matrix Metalloproteinase 9