Repeated exposure of the developing rat brain to magnetic resonance imaging did not affect neurogenesis, cell death or memory function.
The effect of magnetic fields on the brain is a matter of debate. The objective of this study was to investigate whether repeated exposure to strong magnetic fields, such as during magnetic resonance imaging (MRI), could elicit changes in the developing rat brain. Embryonic day 15 (E15) and postnatal day 14 (P14) rats were exposed to MRI using a 7.05 T MR system. The animals were anesthetized and exposed for 35 min per day for 4 successive days. Control animals were anesthetized but no MRI was performed. Body temperature was maintained at 37°C. BrdU was injected after each session (50 mg/kg). One month later, cell proliferation, neurogenesis and astrogenesis in the dentate gyrus were evaluated, revealing no effects of MRI, neither in the E15, nor in the P14 group. DNA damage in the dentate gyrus in the P14 group was evaluated on P18, 1 day after the last session, using TUNEL staining. There was no difference in the number of TUNEL-positive cells after MRI compared with controls, neither in mature neurons, nor in newborn progenitors (BrdU/TUNEL double-labeled cells). Novel object recognition was performed to assess memory function 1 month after MRI. There was no difference in the recognition index observed after MRI compared with the control rats, neither for the E15, nor for the P14 group. In conclusion, repeated exposure to MRI did not appear to affect neurogenesis, cell death or memory function in rats, neither in late gestation (E15-E18) nor in young postnatal (P14-P17) rats.
Center for Brain Repair and Rehabilitation, University of Gothenburg, Sweden.
Changlian
C
Zhu
Jianfeng
J
Gao
Qian
Q
Li
Zhiheng
Z
Huang
Yu
Y
Zhang
Hongfu
H
Li
Hans-Georg
HG
Kuhn
Klas
K
Blomgren
D000818:Animals; D017209:Apoptosis; D001921:Brain / Q000166:cytology / Q000196:embryology*; D002452:Cell Count; D049109:Cell Proliferation; D018891:Dentate Gyrus / Q000166:cytology / Q000196:embryology; D004622:Embryo, Mammalian; D005260:Female; D008279:Magnetic Resonance Imaging / Q000009:adverse effects*; D008568:Memory; D055495:Neurogenesis; D009474:Neurons / Q000166:cytology / Q000502:physiology; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar; D021641:Recognition, Psychology