Biologically active constituents from the fruiting body of Taiwanofungus camphoratus.
Five new benzenoids, benzocamphorins A-E (1-5), and 10 recently isolated triterpenoids, camphoratins A-J (16-25), together with 23 known compounds including seven benzenoids (6-12), three lignans (13-15), and 13 triterpenoids (26-38) were isolated from the fruiting body of Taiwanofungus camphoratus. Their structures were established by spectroscopic analysis. Selected compounds were examined for cytotoxic and anti-inflammatory activities. Compounds 9 and 21 showed moderate cytotoxicity against MCF-7 and Hep2 cell lines with ED(50) values of 3.4 and 3.0μg/mL, respectively. Compounds 21, 25, 26, 29-31, 33, and 36 demonstrated potent anti-inflammatory activity by inhibiting lipopolysaccharide (LPS)-induced nitric oxide (NO) production with IC(50) values of 2.5, 1.6, 3.6, 0.6, 4.1, 4.2, 2.5, and 1.5μM, respectively, which were better than those of the nonspecific nitric oxide synthase (NOS) inhibitor N-nitro-l-arginine methyl ester (l-NAME) (IC(50): 25.8μM). These results may substantiate the use of T. camphoratus in traditional Chinese medicine (TCM) for the treatment of inflammation and cancer-related diseases. The newly discovered compounds deserve further development as anti-inflammatory candidates.
Department of Biotechnology, National Formosa University, Yunlin 632, Taiwan.
Li-Shian
LS
Shi
Chih-Hua
CH
Chao
De-Yang
DY
Shen
Hsiu-Hui
HH
Chan
Chou-Hsiung
CH
Chen
Yu-Ren
YR
Liao
Shwu-Jen
SJ
Wu
Yann-Lii
YL
Leu
Yuh-Chiang
YC
Shen
Yao-Haur
YH
Kuo
E-Jian
EJ
Lee
Keduo
K
Qian
Tian-Shung
TS
Wu
Kuo-Hsiung
KH
Lee
D045744:Cell Line, Tumor; D004354:Drug Screening Assays, Antitumor; D048690:Fruiting Bodies, Fungal / Q000737:chemistry*; D006801:Humans; D020128:Inhibitory Concentration 50; D009682:Magnetic Resonance Spectroscopy; D020072:Polyporales / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization