Novel microbial route to synthesize silver nanoparticles using spore crystal mixture of Bacillus thuringiensis.
Metallic nanoparticles are traditionally synthesized by wet chemical techniques, where the chemicals used are often toxic and flammable. In the present study, the spore crystal mixture of Bacillus thuringiensis was used for the synthesis of silver nanoparticles. Nanoparticles were characterized using UV-Vis absorption spectroscopy, XRD and TEM. X-ray diffraction and TEM analysis showed the average particle size of 15 nm and mixed (cubic and hexagonal) structure. This is for the first time that any bacterial spore crystal mixture was used for the synthesis of nanoparticles. Further, these biologically synthesized nanoparticles were found to be highly toxic against different multi drug resistant human pathogenic bacteria.
Department of Botany, University of Rajasthan, Jaipur 302 004, India.
Devendra
D
Jain
Sumita
S
Kachhwaha
Rohit
R
Jain
Garima
G
Srivastava
S L
SL
Kothari
D001413:Bacillus thuringiensis / Q000737:chemistry*; D003460:Crystallization; D024901:Drug Resistance, Multiple, Bacterial / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000633:toxicity / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D010316:Particle Size; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000254:growth & development; D012834:Silver / Q000737:chemistry*; D013053:Spectrophotometry; D013171:Spores, Bacterial / Q000737:chemistry*; D014961:X-Ray Diffraction
D012834:Silver