Cured of "stickiness", poly-L β-hairpin is promoted with LL-to-DD mutation as a protein and a hydrolase mimic.
The planar ribbon of the poly-L β-hairpin is modified to a local ~90° bend by mutating a cross-strand pair of residues from LL to DD structure. The bend is furnished aromatic side chains in proximity of acid-base-nucleophile side chains, toward the possibility of catalyzed hydrolysis of an active-site-anchored substrate. Six sequences permuted in putative catalytic side chains are evaluated for activity and variability as hydrolase enzymes. Studies using CD, NMR, spectorofluorometry, ITC, and molecular dynamics establish that the sequences over the bent β-hairpin are by and large aggregation-free folds soluble to at least millimolar concentration, and thus remarkably contrasted with "stickiness" of the canonical poly-L β-hairpin. The heterochiral fold displays cooperative ordering and affinity for acetylcholine, p-nitrophenylacetate, and p-nitrophenylphosphate, presumably as the ligands in its aromatic pocket as a bent hairpin. The fold displays hydrolytic activity against p-nitrophenylacetate and manifests saturation kinetics with respect to substrate concentration. However, the catalysis power is feeble, which remains unaffected by repositioning acid-base-nucleophile side chains. Stereochemistry is proven to be critical in the balance between mutually competitive forces of polypeptide structure involved in guidance of folding or aggregation of the structure. Residue stereochemistry is confirmed in its value as the alphabet for design of protein folds to desired molecular shapes.
Department of Chemistry, Indian Institute of Technology Bombay, Mumbai-400076, India.
Kirti
K
Patel
Bhupesh
B
Goyal
Anil
A
Kumar
Nand
N
Kishore
Susheel
S
Durani
D000109:Acetylcholine / Q000737:chemistry; D000595:Amino Acid Sequence; D020134:Catalytic Domain; D006867:Hydrolases / Q000737:chemistry*; D007536:Isomerism; D007700:Kinetics; D056004:Molecular Dynamics Simulation; D008969:Molecular Sequence Data; D009596:Nitrophenols / Q000737:chemistry; D009943:Organophosphorus Compounds / Q000737:chemistry; D010455:Peptides / Q000737:chemistry*; D017510:Protein Folding; D017433:Protein Structure, Secondary
D009596:Nitrophenols; D009943:Organophosphorus Compounds; D010455:Peptides; C008644:nitrophenylphosphate; D006867:Hydrolases; D000109:Acetylcholine