Evaluation of the efficacy of granulocyte colony-stimulating factor for the treatment of experimental myocardial destruction in mice.
We studied the effects of recombinant granulocytic CSF on heart remodeling in BALB/c mice after cryodestruction. Administration of granulocytic CSF was started 1 day after cryodestruction (subcutaneously, 10 μg/kg/day, for 4 days). As early as after the first injection, leukocytosis in the peripheral blood started to develop, leukocyte count peaked on days 4-6 and returned to normal on day 14. Treatment with granulocytic CSF significantly increased the content of progenitor cells in the bone marrow and led to rapid development of the inflammatory reaction and myocardium infiltration with mononuclear cells. Injections of granulocytic CSF did not reduce scar area, but provided significantly less pronounced heart hypertrophy, which attests to its better functional properties. By day 30 after cryodestruction, control animals and animals receiving granulocytic CSF exhibited similar morphological picture at the site of damage. Thus, our regimen of granulocytic CSF administration produced a mobilizing effect on bone marrow progenitor cells and postinfarction heart remodeling. Direct effects of granulocytic CSF on the heart have to be established for its use in the treatment of myocardial infarction.
Institute of Cardiology, Tomsk Research Center, Russian Academy of Medical Sciences, Tomsk, Russia. adm@cardiolog.tomsk.ru
S A
SA
Afanas'ev
Yu V
YV
Rogovskaya
V V
VV
Ryabov
L P
LP
Falaleeva
I N
IN
Sviridov
V P
VP
Shakhov
S V
SV
Popov
R S
RS
Karpov
D000704:Analysis of Variance; D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects*; D016179:Granulocyte Colony-Stimulating Factor / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D006321:Heart / Q000187:drug effects / Q000502:physiology*; D006335:Heart Injuries / Q000188:drug therapy* / Q000473:pathology; D007958:Leukocyte Count; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009206:Myocardium / Q000473:pathology; D012038:Regeneration / Q000187:drug effects / Q000502:physiology*; D018709:Statistics, Nonparametric; D013234:Stem Cells / Q000187:drug effects*
D016179:Granulocyte Colony-Stimulating Factor