Differential responses to natural and recombinant allergens in a murine model of fish allergy.
Aerosolized fish proteins are an important cause of allergic airway reactions in both the domestic and the occupational environment. The aim of this study was to investigate inhalant fish-induced allergy in a mouse model and compare immune responses generated by raw and heat-treated fish extracts as well as natural and recombinant forms of the major fish allergen parvalbumin. Mice were sensitized with raw or cooked pilchard extract and challenged intranasally with cooked pilchard extract, purified natural pilchard parvalbumin or recombinant carp parvalbumin (rCyp c1.01). Cooked pilchard extract predominantly sensitized mice to parvalbumin and induced specific IgG1 and IgE antibodies against both pilchard parvalbumin and rCyp c1.01, whereas additional allergens were recognized by mice sensitized with raw extract, including a 36 kDa allergen that was also recognized by fish processing workers and was identified as glyceraldehyde-3-phosphate dehydrogenase. Mice challenged with cooked extract and purified pilchard parvalbumin had increased Th2 cytokine production in mediastinal lymph node cells and splenocytes, whereas mice challenged with rCyp c1.01 did not. This study identifies a new IgE-binding protein that may be important in occupational allergy to fish and demonstrates the feasibility of testing recombinant allergens for immunotherapeutic potential in vivo.
Division of Immunology, Institute of Infectious Disease and Molecular Medicine, Health Science Faculty, University of Cape Town, Cape Town, South Africa.
Michelle L
ML
van der Ventel
Natalie E
NE
Nieuwenhuizen
Frank
F
Kirstein
Christoph
C
Hikuam
Mohamed F
MF
Jeebhay
Ines
I
Swoboda
Frank
F
Brombacher
Andreas L
AL
Lopata
D000281:Administration, Intranasal; D000485:Allergens / Q000008:administration & dosage / Q000276:immunology*; D000595:Amino Acid Sequence; D000818:Animals; D000917:Antibody Formation / Q000276:immunology; D000918:Antibody Specificity / Q000276:immunology; D016535:Bronchial Hyperreactivity / Q000150:complications / Q000276:immunology; D004195:Disease Models, Animal; D000939:Epitopes / Q000276:immunology; D005260:Female; D029941:Fish Proteins / Q000008:administration & dosage / Q000276:immunology*; D005512:Food Hypersensitivity / Q000150:complications / Q000276:immunology*; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000737:chemistry / Q000276:immunology; D006801:Humans; D007114:Immunization; D013058:Mass Spectrometry; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D010320:Parvalbumins / Q000276:immunology; D010455:Peptides / Q000737:chemistry; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000166:cytology / Q000276:immunology; D014020:Tissue Extracts
D000485:Allergens; D000939:Epitopes; D029941:Fish Proteins; D010320:Parvalbumins; D010455:Peptides; D011994:Recombinant Proteins; D014020:Tissue Extracts; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases