Synthetic biotinylated tetra β(1→5) galactofuranoside for in vitro aspergillosis diagnosis.
The synthesis of a tetra β(1→5) galactofuranoside was achieved using a thioglycoside donor with a methyl tert-butyl phenyl thio leaving group. This tetrasaccharide was conjugated to biotin and validated as antigen with the monoclonal antibody used for clinical detection of Aspergillus fumigatus galactomannan on streptavidin-coated microplates. Then we have shown its ability to detect antibodies associated with A. fumigatus induced disease by using sera from patients with Allergic broncho-pulmonary aspergillosis (ABPA) and correlated the results of antibody detection with those gained with a commercially available diagnostic test.
UPMC-ENS-CNRS, Laboratoire de BioMolécules, UMR 7203, Fédération de Chimie Moléculaire FR 2769, Département de Chimie, Ecole Normale Supérieure, 24 rue Lhomond, 75005 Paris, France.
Laurent
L
Cattiaux
Boualem
B
Sendid
Mayeul
M
Collot
Emeline
E
Machez
Daniel
D
Poulain
Jean-Maurice
JM
Mallet
D000818:Animals; D000908:Antibodies, Fungal / Q000097:blood; D001228:Aspergillosis / Q000175:diagnosis*; D001232:Aspergillus fumigatus / Q000276:immunology; D001710:Biotin / Q000737:chemistry*; D005697:Galactosides / Q000138:chemical synthesis; D006801:Humans; D066298:In Vitro Techniques; D051381:Rats
D000908:Antibodies, Fungal; D005697:Galactosides; D001710:Biotin