High-resolution simultaneous voltage and Ca2+ imaging.
Combining voltage and Ca(2+) imaging allows the correlation of electrical and chemical activity at sub-cellular level. Here we describe a novel apparatus designed to obtain simultaneous voltage and Ca(2+) measurements with single-trial resolution from sites as small as a few microns. These measurements can be obtained with negligible optical cross-talk between the two signals and negligible photo-damage of the preparation. The capability of the technique was assessed recording either from individual neurons in brain slices or from networks of cultured neurons. The present achievements open the gate to many novel physiological investigations requiring simultaneous measurement of voltage and Ca(2+) signals.
Division of Pharmacology and Neurobiology, Biozentrum-University of Basel, Basel, Switzerland.
Kaspar E
KE
Vogt
Stephan
S
Gerharz
Jeremy
J
Graham
Marco
M
Canepari
D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D020013:Calcium Signaling / Q000187:drug effects / Q000502:physiology*; D002531:Cerebellum / Q000166:cytology / Q000502:physiology; D004594:Electrophysiology / Q000295:instrumentation / Q000379:methods; D019706:Excitatory Postsynaptic Potentials / Q000502:physiology; D005456:Fluorescent Dyes / Q000378:metabolism; D005470:Fluorometry / Q000295:instrumentation / Q000379:methods*; D016257:Fura-2 / Q000031:analogs & derivatives / Q000378:metabolism; D006624:Hippocampus / Q000166:cytology / Q000502:physiology; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods; D010852:Picrotoxin / Q000494:pharmacology; D011689:Purkinje Cells / Q000502:physiology; D017966:Pyramidal Cells / Q000187:drug effects / Q000502:physiology; D011726:Pyridinium Compounds / Q000378:metabolism; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods; D013343:Styrenes / Q000378:metabolism
D005456:Fluorescent Dyes; D016257:Fura-2; D010852:Picrotoxin; D011726:Pyridinium Compounds; D013343:Styrenes; C089669:1-(3-sulfonatopropyl)-4-(beta-(2-(di-n-octylamino)-6-naphthyl)vinyl)pyridinium betaine; C099388:JPW 1114