Preparation and characterization of a thermostable and biodegradable biopolymers using natural cross-linker.
The present study describes preparation and characterization of a thermally stable and biodegradable biopolymer using collagen and a natural polymer, alginic acid (AA). Required concentration of alginic acid and collagen was optimized and the resulting biopolymer was characterized for, degree of cross-linking, mechanical strength, thermal stability, biocompatibility (toxicity) and biodegradability. Results reveal, the degree of cross-linking of alginic acid (at 1.5% concentration) with collagen was calculated as 75%, whereas it was 83% with standard cross-linking agent, glutaraldehyde (at 1.5% concentration). The AA cross-linked biopolymer was stable up to 245°C and Exhibits 5-6-fold increase in mechanical (tensile) strength compared to plain collagen (native) materials. However, glutaraldehyde cross-linked material exhibits comparatively less thermal stability and brittle in nature (low tensile strength). With regard to cell toxicity, no cytotoxicity was observed for AA cross-linked material when tested with mesenchymal cells and found degradable when treated with collagenase enzyme. The nature of bonding pattern and the reason for thermal stability of AA cross-linked collagen biopolymer was discussed in detail with the help of bioinformatics. A supplementary file on efficacy of AACC as a wound dressing material is demonstrated in detail with animal model studies.
Microbiology Division, Central Leather Research Institute, (CSIR, New Delhi), Adyar, Chennai 20, Tamil Nadu, India.
Tapas
T
Mitra
G
G
Sailakshmi
A
A
Gnanamani
S Thirupathi Kumara
ST
Raja
T
T
Thiruselvi
V Mangala
VM
Gowri
Naga Vignesh
NV
Selvaraj
Gopal
G
Ramesh
A B
AB
Mandal
D000464:Alginates / Q000737:chemistry; D000818:Animals; D001673:Biodegradation, Environmental; D001704:Biopolymers / Q000737:chemistry*; D002152:Calorimetry, Differential Scanning; D002417:Cattle; D003094:Collagen / Q000737:chemistry / Q000648:ultrastructure; D003432:Cross-Linking Reagents / Q000737:chemistry*; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006860:Hydrogen Bonding; D006909:Hydroxyproline / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology; D008958:Models, Molecular; D012995:Solubility; D013696:Temperature; D013718:Tensile Strength; D013818:Thermogravimetry
D000464:Alginates; D001704:Biopolymers; D003432:Cross-Linking Reagents; D006603:Hexuronic Acids; D020723:Glucuronic Acid; D003094:Collagen; D006909:Hydroxyproline