Insulin-like growth factor 1 protects vestibular hair cells  from aminoglycosides.
This study investigated the therapeutic potential of insulin-like growth factor-1 (IGF-1) for vestibular hair cells using explant cultures of mouse utricles. After incubation with the ototoxic drug gentamicin, explants from neonatal mouse utricles were cultured in medium containing IGF-1 at various concentrations. Histological evaluation revealed significant increases in the number of surviving hair cells cultured with IGF-1 at concentrations reflecting a clinical setting. Immunostaining for trio-binding protein and espin showed the maintenance of functional structures in hair bundles at the apex of surviving hair cells. An FM1-43 assay indicated the presence of mechanoelectrical transduction channels in surviving hair cells. These findings indicate that IGF-1 may protect the functionality of vestibular hair cells against drug-induced injury.
Department of Otolaryngology and Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
Nakarin
N
Angunsri
Akiko
A
Taura
Takayuki
T
Nakagawa
Yushi
Y
Hayashi
Shin-ichiro
S
Kitajiri
Eigo
E
Omi
Kazuo
K
Ishikawa
Juichi
J
Ito
D000617:Aminoglycosides / Q000633:toxicity*; D000818:Animals; D005839:Gentamicins / Q000633:toxicity; D018069:Hair Cells, Vestibular / Q000187:drug effects* / Q000378:metabolism; D007150:Immunohistochemistry; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D040542:Mechanotransduction, Cellular / Q000187:drug effects / Q000502:physiology; D051379:Mice; D008813:Mice, Inbred ICR; D018613:Microscopy, Confocal; D018696:Neuroprotective Agents / Q000494:pharmacology*; D009924:Organ Culture Techniques; D012444:Saccule and Utricle / Q000187:drug effects / Q000378:metabolism
D000617:Aminoglycosides; D005839:Gentamicins; D018696:Neuroprotective Agents; D007334:Insulin-Like Growth Factor I