Adjuvant effect of Gantrez®AN nanoparticles during oral vaccination of piglets against F4+enterotoxigenic Escherichia coli.
In this study, the adjuvanticity of methylvinylether-co-maleic anhydride (Gantrez(®)AN) nanoparticles (NP) was investigated in an oral immunisation experiment of pigs against F4+enterotoxigenic Escherichia coli (F4+ETEC). In addition, Wheat Germ Agglutinin (WGA)-coating of the nanoparticles was tested for enterocyte-targeting. Pigs were either vaccinated with F4 fimbriae, F4 encapsulated in Gantrez(®)AN NP, F4 encapsulated in Gantrez(®)AN NP coated with WGA or F4 fimbriae mixed with empty Gantrez(®)AN NP. Only vaccination with the combination of F4 mixed with empty Gantrez(®)AN NP improved protection against F4+ETEC infection. In addition, vaccination with this formulation also resulted in an F4-specific serum antibody response prior to F4+ETEC challenge. Encapsulation of F4 in Gantrez(®)AN NP only raised the serum antibody response after F4+ETEC challenge compared to soluble F4, but did not improve protection, whereas WGA-coating almost completely abolished the serum antibody response. These data indicate that nanoparticle effects after F4 encapsulation were of lesser importance for the adjuvant effect of Gantrez(®)AN NP, contrarily to the reactivity of the Gantrez(®)AN polymer used to prepare the nanoparticles.
Ghent University, Laboratory of Pharmaceutical Technology, Harelbekestraat 72, B-9000 Ghent, Belgium.
Katrien
K
Vandamme
Vesna
V
Melkebeek
Melkebeek
M
Vesna
Eric
E
Cox
Cox
C
Eric
Jean Paul
JP
Remon
Remon Jean
RJ
Paul
Chris
C
Vervaet
Vervaet
V
Chris
D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000284:Administration, Oral; D000818:Animals; D057138:Bacterial Shedding; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000276:immunology*; D054307:Enterotoxigenic Escherichia coli / Q000276:immunology*; D004927:Escherichia coli Infections / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary*; D005243:Feces / Q000382:microbiology; D036781:Fimbriae Proteins / Q000276:immunology; D008298:Maleates / Q000276:immunology*; D053758:Nanoparticles; D011145:Polyvinyls; D013552:Swine; D013553:Swine Diseases / Q000517:prevention & control*; D014909:Wheat Germ Agglutinins
D000276:Adjuvants, Immunologic; D001428:Bacterial Vaccines; D008298:Maleates; D011145:Polyvinyls; D014909:Wheat Germ Agglutinins; C058026:polyvinylmethoxyethylene-maleic anhydride copolymer; D036781:Fimbriae Proteins