Identification and isolation of Genotype-I Japanese encephalitis virus from encephalitis patients.
Historically, Japanese Encephalitis virus (JEV) genotype III (GIII) has been responsible for human diseases. In recent years, JEV genotype I (GI) has been isolated from mosquitoes collected in numerous countries, but has not been isolated from patients with encephalitis. In this study, we report recovery of JEV GI live virus and identification of JEV GI RNA from cerebrospinal fluid (CSF) of encephalitis patients in JE endemic areas of China. Whole-genome sequencing and molecular phylogenetic analysis of the JEV isolate from the CSF samples was performed. The isolate in this study is highly similar to other JEV GI strains which isolated from mosquitoes at both the nucleotide and deduced amino acid levels. Phylogenetic analysis based on the genomic sequence showed that the isolate belongs to JEV GI, which is consistent with the phylogenetic analysis based on the pre-membrane (PrM) and Glycoprotein genes. As a conclusion, this is the first time to isolate JEV GI strain from CSF samples of encephalitis patients, so continuous survey and evaluate the infectivity and pathogenecity of JEV GI strains are necessary, especially for the JEV GI strains from encephalitis patients. With respect to the latter, because all current JEV vaccines (live and inactivated are derived from JEV GIII strains, future studies should be aimed at investigating and monitoring cross-protection of the human JEV GI isolates against widely used JEV vaccines.
State Key Laboratory for Infectious Disease Prevention and Control (SKLID), Institute for Viral Disease Control and Prevention, China CDC, Beijing 100052, PR China. wanglih9755@yahoo.com.cn
Lihua
L
Wang
Shihong
S
Fu
Hailin
H
Zhang
Xufang
X
Ye
Deshan
D
Yu
Zhang
Z
Deng
Jun
J
Yuan
Yougang
Y
Zhai
Minghua
M
Li
Zhi
Z
Lv
Weixin
W
Chen
Hongyue
H
Jiang
Xiaoyan
X
Gao
Yuxi
Y
Cao
Huanyu
H
Wang
Qing
Q
Tang
Guodong
G
Liang
D000293:Adolescent; D000328:Adult; D002555:Cerebrospinal Fluid / Q000821:virology; D002648:Child; D002681:China / Q000453:epidemiology; D016000:Cluster Analysis; D004664:Encephalitis Virus, Japanese / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D004672:Encephalitis, Japanese / Q000453:epidemiology* / Q000821:virology*; D019353:Endemic Diseases; D005260:Female; D016679:Genome, Viral; D005838:Genotype; D006801:Humans; D007223:Infant; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000235:genetics
D012367:RNA, Viral; D014764:Viral Proteins