The cross-sectional association between insulin resistance and circulating complement C3 is partly explained by plasma alanine aminotransferase, independent of central obesity and general inflammation (the CODAM study).
BACKGROUND
Complement C3, a central component of the innate immune system is increased in subjects with obesity and type 2 diabetes and is a novel risk factor for cardiovascular disease. We hypothesized that the strong association between insulin resistance and circulating amounts of C3 may be related to hepatic fat accumulation -independent of central obesity itself and of a general low-grade inflammatory response.


OBJECTIVE
To what extent is the association between insulin resistance and C3 explained by plasma levels of alanine aminotransferase (ALT) as a surrogate of hepatic fat accumulation.


METHODS
Cross-sectional analyses conducted in the Cohort on Diabetes and Atherosclerosis Maastricht (CODAM) study. Analyses were restricted to subjects with none-to-moderate alcohol consumption (n = 453, 61·4% men). Multiple linear regression analyses were used to investigate the association between HOMA2IR (main determinant) and circulating complement C3 (main outcome), and the mediating role of ALT herein. All analyses were adjusted for age, sex, presence of type 2 diabetes mellitus or heart disease, use of medication, smoking, alcohol consumption, waist circumference and inflammation.


RESULTS
Insulin resistance (estimated as HOMA2IR) was strongly associated with circulating C3 (standardized regression coefficient β 0·40 [95% CI: 0·30; 0·49]) and also with ALT (β 0·44 [0·34; 0·54]), both adjusted for the above-mentioned covariates. The association between HOMA2IR and C3 was attenuated after further adjustment for ALT (β decreased to 0·34 [0·24; 0·44]).


CONCLUSIONS
Plasma ALT can explain 14·2% of the strong association between insulin resistance and circulating concentrations of complement C3, independent of central obesity and general inflammation.
Cardiovascular Research Institute Maastricht (CARIM), Maastricht University, Maastricht, The Netherlands. m.vangreevenbroek@intmed.unimaas.nl
Marleen M J
MM
van Greevenbroek
Marjon
M
Jacobs
Carla J H
CJ
van der Kallen
Vicky M M-J
VM
Vermeulen
Eugene H J M
EH
Jansen
Casper G
CG
Schalkwijk
Isabel
I
Ferreira
Edith J M
EJ
Feskens
Coen D A
CD
Stehouwer
D000368:Aged; D000410:Alanine Transaminase / Q000097:blood*; D015415:Biomarkers / Q000378:metabolism; D015992:Body Mass Index; D015331:Cohort Studies; D003176:Complement C3 / Q000378:metabolism*; D003430:Cross-Sectional Studies; D005260:Female; D006801:Humans; D007249:Inflammation / Q000150:complications; D007333:Insulin Resistance / Q000502:physiology*; D008297:Male; D008875:Middle Aged; D009426:Netherlands; D056128:Obesity, Abdominal / Q000150:complications*; D012044:Regression Analysis; D044465:White People
D015415:Biomarkers; D003176:Complement C3; D000410:Alanine Transaminase