Release of soluble protein from peanut (Arachis hypogaea, Leguminosae) and its adsorption by activated charcoal.
Peanut (Arachis hypogaea, Leguminosae) allergy is a major cause of food-induced anaphylaxis. The potential use of activated charcoal (AC) to adsorb and reduce the bioavailability of peanut protein allergens for use in the moderation of hypersensitivity reactions was investigated. The rate and extent of protein release from peanut and the adsorption of the solubilized protein by AC was determined under physiological pH values and confirmed in vivo using a porcine animal model system. Peanut proteins were adsorbed with equal efficiency at pH 2 and 7 and are completely removed from solution by an AC/protein ratio of approximately 80:1. This suggests that AC can bind protein under gastric (pH 2) or intestinal (pH 7) conditions. The rapid adsorption of soluble peanut allergens and the continuous binding of allergens released from peanut particulate material suggest the potential efficacy of using AC for gastric decontamination and possible elimination of a biphasic allergic reaction.
Department of Chemistry, Hendrix College, Conway, Arkansas, USA. kopper@hendrix.edu
Randall
R
Kopper
Trang
T
Van
Ara
A
Kim
Ricki
R
Helm
D000327:Adsorption; D000818:Animals; D052179:Antigens, Plant / Q000737:chemistry*; D010367:Arachis / Q000737:chemistry*; D002606:Charcoal / Q000737:chemistry*; D005512:Food Hypersensitivity / Q000517:prevention & control; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010940:Plant Proteins / Q000737:chemistry*; D012995:Solubility; D013552:Swine
D052179:Antigens, Plant; D010940:Plant Proteins; D002606:Charcoal