Molecular cloning and differential expression patterns of copper/zinc superoxide dismutase and manganese superoxide dismutase in Hypophthalmichthys molitrix.
Copper/zinc superoxide dismutase (Cu,Zn-SOD) cDNA and manganese superoxide dismutase (Mn-SOD) cDNA were first cloned from silver carp Hypophthalmichthys molitrix using reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) method. The open reading frame (ORF) of Cu,Zn-SOD is 465 bp and encodes a 154 amino acids (aa) protein, whereas the ORF of Mn-SOD is 675 bp and encodes a 224 aa protein. Multiple polypeptide sequence alignment showed high identity both of Cu,Zn-SOD (70-87%) and Mn-SOD (80-96%) with the species compared. Both Cu,Zn-SOD and Mn-SOD were detected in heart, brain, liver, kidney, spleen, muscle, gill and blood. Cu,Zn-SOD and Mn-SOD were expressed throughout the embryogenesis, indicating their important roles during embryonic development specially at the cleavage stage. Acute hypoxia suppressed expression of Cu,Zn-SOD and Mn-SOD in liver significantly, up-regulated them in gill relatively, indicating that tissue-specific expression of Cu,Zn-SOD and Mn-SOD is an important stress response adapted to hypoxia.
Yangtze River Fisheries Research Institute, The Chinese Academy of Fisheries Sciences, Jingzhou, Hubei 434000, China.
Zhi-Wei
ZW
Zhang
Zhong
Z
Li
Hong-Wei
HW
Liang
Lin
L
Li
Xiang-Zhong
XZ
Luo
Gui-Wei
GW
Zou
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002347:Carps / Q000145:classification / Q000196:embryology / Q000502:physiology*; D003001:Cloning, Molecular; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic; D000860:Hypoxia / Q000201:enzymology; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment; D013482:Superoxide Dismutase / Q000235:genetics / Q000276:immunology
D013482:Superoxide Dismutase