Symplasmic transport and phloem loading in gymnosperm leaves.
Despite more than 130 years of research, phloem loading is far from being understood in gymnosperms. In part this is due to the special architecture of their leaves. They differ from angiosperm leaves among others by having a transfusion tissue between bundle sheath and the axial vascular elements. This article reviews the somewhat inaccessible and/or neglected literature and identifies the key points for pre-phloem transport and loading of photoassimilates. The pre-phloem pathway of assimilates is structurally characterized by a high number of plasmodesmata between all cell types starting in the mesophyll and continuing via bundle sheath, transfusion parenchyma, Strasburger cells up to the sieve elements. Occurrence of median cavities and branching indicates that primary plasmodesmata get secondarily modified and multiplied during expansion growth. Only functional tests can elucidate whether this symplasmic pathway is indeed continuous for assimilates, and if phloem loading in gymnosperms is comparable with the symplasmic loading mode in many angiosperm trees. In contrast to angiosperms, the bundle sheath has properties of an endodermis and is equipped with Casparian strips or other wall modifications that form a domain border for any apoplasmic transport. It constitutes a key point of control for nutrient transport, where the opposing flow of mineral nutrients and photoassimilates has to be accommodated in each single cell, bringing to mind the principle of a revolving door. The review lists a number of experiments needed to elucidate the mode of phloem loading in gymnosperms.
Department of Plant Biology and Biotechnology, University of Copenhagen, Thorvaldsensvej 40, DK-1871, Frederiksberg C, Denmark.
Johannes
J
Liesche
Helle Juel
HJ
Martens
Alexander
A
Schulz
D001692:Biological Transport; D002462:Cell Membrane / Q000378:metabolism; D032462:Cycadopsida / Q000033:anatomy & histology / Q000166:cytology / Q000378:metabolism*; D017136:Ion Transport; D052585:Phloem / Q000378:metabolism*; D018515:Plant Leaves / Q000033:anatomy & histology / Q000378:metabolism*; D031425:Plasmodesmata / Q000378:metabolism / Q000648:ultrastructure; D013395:Sucrose / Q000378:metabolism; D014867:Water / Q000378:metabolism
D014867:Water; D013395:Sucrose