Anti-inflammatory effect of visnagin in lipopolysaccharide-stimulated BV-2 microglial cells.
Visnagin, which is found in Ammi visnaga, has biological activity as a vasodilator and reduces blood pressure by inhibiting calcium influx into the cell. The present study demonstrates the anti-inflammatory effect of visnagin on lipopolysaccharide (LPS)-stimulated BV-2 microglial cells. When cells were treated with visnagin prior to LPS stimulation, production of nitric oxide and expression of iNOS were attenuated in a dose-dependent manner. Visnagin also caused a significant decrease of mRNA expression and release of TNF-α, IL-1β and IFNγ. In addition, visnagin reduced LPS-induced IL-6 and MCP-1 mRNA level. We further found that visnagin dose-dependently inhibited LPS-induced AP-1 and NF-κB luciferase activities. Taken together, our results for the first time suggest that the anti-inflammatory effect of visnagin might result from the inhibition of transcription factors, such as AP-1 and NF-κB.
Institute of Natural Medicine, Hallym University, Chuncheon, Korea.
Jin-Koo
JK
Lee
Jun-Sub
JS
Jung
Sang-Hee
SH
Park
Soo-Hyun
SH
Park
Yun-Beom
YB
Sim
Seon-Mi
SM
Kim
Tal-Soo
TS
Ha
Hong-Won
HW
Suh
D031165:Ammi / Q000737:chemistry; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D002460:Cell Line; D016207:Cytokines / Q000378:metabolism; D005638:Fruit; D007249:Inflammation / Q000188:drug therapy; D007666:Khellin / Q000031:analogs & derivatives* / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000276:immunology; D051379:Mice; D017628:Microglia / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D008517:Phytotherapy; D018808:Transcription Factor AP-1 / Q000378:metabolism
D000894:Anti-Inflammatory Agents, Non-Steroidal; D016207:Cytokines; D008070:Lipopolysaccharides; D016328:NF-kappa B; D018808:Transcription Factor AP-1; D009569:Nitric Oxide; D007666:Khellin; C496319:NOS2 protein, human; D052247:Nitric Oxide Synthase Type II; C044999:visnagin