Amaranth (Amaranthus hypochondriacus) vicilin subunit structure.
The 7S-globulin fraction is a minor component of the amaranth storage proteins. The present work provides new information about this protein. The amaranth 7S-globulin or vicilin presented a sedimentation coefficient of 8.6 ± 0.6 S and was composed of main subunits of 66, 52, 38, and 16 kDa. On the basis of mass spectrometry (MS) analysis of tryptic fragments, the 52, 38, and 16 kDa subunits presented sequence homology with sesame vicilin, whereas the 66 kDa subunit showed sequence similarity with a putative vicilin. Several characteristics of the 66 kDa subunit were similar to members of the convicilin family. Results support the hypothesis that the 7S-globulin molecules are composed of subunits coming from at least two gene families with primary products of 66 and 52 kDa, respectively. According to the present information, amaranth vicilin may be classified into the vicilin group that includes pea, broad bean, and sesame vicilins, among others.
Facultad de Ciencias Exactas, Centro de Investigación y Desarrollo en Criotecnología de Alimentos, Universidad Nacional de La Plata y Consejo Nacional de Investigaciones Científicas y Técnicas, calle 47 y 116, 1900 La Plata, Argentina.
Alejandra
A
Quiroga
E Nora
EN
Martínez
Hélène
H
Rogniaux
Audrey
A
Geairon
M Cristina
MC
Añón
D027721:Amaranthus / Q000737:chemistry*; D000595:Amino Acid Sequence; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010449:Peptide Mapping; D021122:Protein Subunits / Q000737:chemistry*; D055314:Seed Storage Proteins / Q000737:chemistry*; D016415:Sequence Alignment
D021122:Protein Subunits; D055314:Seed Storage Proteins; C015575:vicilin protein, plant