Stability-indicating TLC-densitometric method for estimation of dexrabeprazole and domperidone in pharmaceutical dosage form.
A stability-indicating thin layer chromatographic (TLC) method for determination of dexrabeprazole (DEX) and domperidone (DOM) in combined dosage form has been developed and validated. The mobile phase selected was acetone:toluene:methanol (4.5:4.5:0.5, v/v/v) with ultraviolet (UV) detection at 285 nm. The retention factors for DEX and DOM were found to be 0.49 ± 0.02 and 0.24 ± 0.03, respectively. The method was validated with respect to linearity, accuracy, precision, and robustness. The linearity range for DEX was 50-350 ng/band (r² = .9960) and for DOM was 100-700-n /band (r² = .9982), respectively. The method was successfully applied for the analysis of drugs in pharmaceutical formulation.
Padmashri Dr. D.Y. Patil Institute of Pharmceutical Sciences and Research, Sant Tukaram Nagar, Pimpri, Pune, Maharashtra, India. sohanchitlange@rediffmail.com
Sohan S
SS
Chitlange
Amir I
AI
Mulla
Ganesh R
GR
Pawbake
Sagar B
SB
Wankhede
D053799:2-Pyridinylmethylsulfinylbenzimidazoles / Q000032:analysis*; D002855:Chromatography, Thin Layer / Q000379:methods*; D003720:Densitometry / Q000379:methods*; D004294:Domperidone / Q000032:analysis*; D004304:Dosage Forms; D004338:Drug Combinations; D016014:Linear Models; D064750:Rabeprazole; D012680:Sensitivity and Specificity
D053799:2-Pyridinylmethylsulfinylbenzimidazoles; D004304:Dosage Forms; D004338:Drug Combinations; D064750:Rabeprazole; D004294:Domperidone