HNP-3 enhanced the antimicrobial activity of CIP by promoting ATP efflux from P. aeruginosa cells.
To establish a novel strategy of P. aeruginosa control, we acquired recombination HNP-3 by gene recombination. Then we examined HNP-3 bio-activities and the influences of antimicrobial peptide on the efflux of ATP. Consequently, we obtained target protein with a molecular mass of 3,000 D consistent with the Anticipation. FIC index of Ciprofloxacin added HNP-3 was less than 0.5 and HNP3 synergistically cooperated with CIP to suppress P. aeruginosa colony formation revealed that there was significant synergy. ATP efflux was however up-regulated by low concentrations of HNP-3, although CIP did not exert any influence on ATP efflux. Conclusively, recombination protein HNP-3 displayed antimicrobial and synergic effects. HNP-3 enhanced the antimicrobial activity of CIP by promoting ATP efflux from P. aeruginosa cells and decreasing efflux of the drugs, which could have useful clinical applications.
Department of Geriatrics, West China Hospital, Sichuan University, Chengdu, 610041, China.
Hao
H
Wang
Birong
B
Dong
Li
L
Lou
D000255:Adenosine Triphosphate / Q000378:metabolism; D000890:Anti-Infective Agents / Q000378:metabolism / Q000494:pharmacology*; D002939:Ciprofloxacin / Q000378:metabolism / Q000494:pharmacology*; D015169:Colony Count, Microbial; D017931:DNA Primers / Q000235:genetics; D015151:Immunoblotting; D010957:Plasmids / Q000235:genetics; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism / Q000494:pharmacology*; D023084:alpha-Defensins / Q000378:metabolism / Q000494:pharmacology*
D000890:Anti-Infective Agents; D017931:DNA Primers; D011994:Recombinant Proteins; D023084:alpha-Defensins; C406427:human neutrophil peptide 3; D002939:Ciprofloxacin; D000255:Adenosine Triphosphate