Heterobimetallic Ru(II)-Eu(III) complex as chemodosimeter for selective biogenic amine odorants detection in fish sample.
Gaseous biogenic amines such as putrescine, spermidine, aniline, and trimethylamine are important biomolecules that play many crucial roles in metabolism and medical diagnostics. A chemodosimetric detection assay has been developed for those gaseous amines by Ru(II)-Eu(III) heterobimetallic complexes, K{[Ru(II)((t)Bubpy)(CN)(4)](2)Eu(III)(H(2)O)(4)} (where (t)Bubpy = 4,4'-di-tert-butyl-2,2'-bipyridine). Synthesis, X-ray crystal characterization, and spectroscopic properties of this Ru(II)-Eu(III) heterobimetallic complex were reported. Binding properties of the Ru(II)-Eu(III) complex with common gases revealed that this complex is very selective to gaseous amine molecules. Sensitivity of this complex toward the amines was found as ∼log k() = 4.5-4.8. Real time monitoring of gaseous biogenic amines was applied to real fish samples (Atlantic mackerel) by studying the spectrofluorimetric responses of the Ru(II)-Eu(III) complex toward different biogenic amine concentration. GC/MS studies were also used as a reference for the studies. A linear spectrofluorimetric response was found toward biogenic amine concentration in real fish samples. This complex was found to respond specifically to those biogenic amines down to 10 ppb.
Department of Biology and Chemistry, City University of Hong Kong, 83 Tat Chee Avenue, Hong Kong SAR, China. cfchow@cityu.edu.hk
Cheuk-Fai
CF
Chow
Hoi-Kuan
HK
Kong
Shu-Wai
SW
Leung
Brenda K W
BK
Chiu
Chi-Kin
CK
Koo
Elva N Y
EN
Lei
Michael H W
MH
Lam
Wing-Tak
WT
Wong
Wai-Yeung
WY
Wong
D000042:Absorption; D000818:Animals; D001679:Biogenic Amines / Q000032:analysis*; D002623:Chemistry Techniques, Analytical / Q000295:instrumentation*; D004583:Electrons; D005063:Europium / Q000737:chemistry*; D005399:Fishes; D005740:Gases / Q000737:chemistry; D008163:Luminescent Measurements; D008958:Models, Molecular; D008968:Molecular Conformation; D009812:Odorants / Q000032:analysis*; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D012428:Ruthenium / Q000737:chemistry*; D013379:Substrate Specificity; D014835:Volatilization
D001679:Biogenic Amines; D005740:Gases; D009942:Organometallic Compounds; D005063:Europium; D012428:Ruthenium