The ODN probes conjugating the Cu(II) complex enhance the luminol chemiluminescence by assembling on the DNA template.
Potent peroxidase-like activity of the β-ketoenamine (1)-dicopper (II) complex (2) for the chemiluminescence (CL) of luminol either in the presence or absence of H(2)O(2) has been previously demonstrated by our group. In this study, the β-ketoenamine (1) as the ligand unit for copper(II) was incorporated into the oligonucleotide (ODN) probes. It has been shown that the catalytic activity of the ODN probes conjugating the ligand-Cu(II) complex is activated by hybridization with the target DNA with the complementary sequence. Thus, this study has successfully demonstrated the basic concept for the sensitive detection of nucleic acids by CL based on the template-inductive activation of the catalytic unit for CL.
Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1, Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
Yosuke
Y
Taniguchi
Akiko
A
Nitta
Sun Min
SM
Park
Akiko
A
Kohara
Takahiro
T
Uzu
Shigeki
S
Sasaki
D056831:Coordination Complexes / Q000378:metabolism; D003300:Copper; D004247:DNA / Q000032:analysis* / Q000378:metabolism; D008024:Ligands; D008163:Luminescent Measurements; D008165:Luminol; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes / Q000737:chemistry*; D013698:Templates, Genetic
D056831:Coordination Complexes; D008024:Ligands; D015345:Oligonucleotide Probes; D008165:Luminol; D003300:Copper; D004247:DNA