Cyclin E is recruited to the nuclear matrix during differentiation, but is not recruited in cancer cells.
Cyclin E supports pre-replication complex (pre-RC) assembly, while cyclin A-associated kinase activates DNA synthesis. We show that cyclin E, but not A, is mounted upon the nuclear matrix in sub-nuclear foci in differentiated vertebrate cells, but not in undifferentiated cells or cancer cells. In murine embryonic stem cells, Xenopus embryos and human urothelial cells, cyclin E is recruited to the nuclear matrix as cells differentiate and this can be manipulated in vitro. This suggests that pre-RC assembly becomes spatially restricted as template usage is defined. Furthermore, failure to become restricted may contribute to the plasticity of cancer cells.
Department of Biology, University of York, YO10 5YW and School of Medicine, Leeds University, LS2 9JT, UK.
Jennifer
J
Munkley
Nikki A
NA
Copeland
Victoria
V
Moignard
John R P
JR
Knight
Erin
E
Greaves
Simon A
SA
Ramsbottom
Mary E
ME
Pownall
Jennifer
J
Southgate
Justin F-X
JF
Ainscough
Dawn
D
Coverley
D021581:Active Transport, Cell Nucleus; D000818:Animals; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D019927:Cyclin E / Q000378:metabolism*; D006801:Humans; D051379:Mice; D009369:Neoplasms / Q000378:metabolism*; D015530:Nuclear Matrix / Q000378:metabolism*; D021381:Protein Transport; D014982:Xenopus laevis
D019927:Cyclin E