Effects of photodynamic exposure on endothelial cells in vitro.
Experiments on cultured human umbilical vein endotheliocytes showed that accumulation of photosensitive dye (aluminum phthalocyanin; PHOTOSENSE) in cells and laser exposure alone were inessential for the viability of endothelial cells. Contrary to this, exposure of the cells which have accumulated aluminum phthalocyanin (an average of 111.1 ng/mg protein) to low-intensity laser (λ=675 nm) led to a dose-dependent reduction of endotheliocyte viability. Hence, cultured endothelial cells can be used for screening of various photosensitizers and preliminary optimization of photodynamic therapy.
Institute of Experimental Cardiology, Russian Cardiology Research-and-Production Complex, Federal Agency for High-Technology Medical Care, Moscow, Russia. andreeva-er@mail.ru
E R
ER
Andreeva
O O
OO
Udartseva
I N
IN
Vozovikov
S G
SG
Kuzmin
E M
EM
Tararak
D002470:Cell Survival / Q000187:drug effects; D042783:Endothelial Cells / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D007211:Indoles / Q000493:pharmacokinetics / Q000633:toxicity*; D053685:Laser Therapy / Q000379:methods*; D008856:Microscopy, Fluorescence; D009942:Organometallic Compounds / Q000493:pharmacokinetics / Q000633:toxicity*; D010778:Photochemotherapy / Q000379:methods*; D013778:Tetrazolium Salts; D013844:Thiazoles; D014471:Umbilical Veins / Q000166:cytology
D007211:Indoles; D009942:Organometallic Compounds; D013778:Tetrazolium Salts; D013844:Thiazoles; C060570:aluminum phthalocyanine; C022616:thiazolyl blue