A modified supported bilayer/diblock polymer--working towards a tunable coating for capillary electrophoresis.
A surfactant bilayer/diblock polymer coating was previously developed for the separation of proteins. The coating consisted of a mixture of the cationic surfactant dioctadecyldimethylammonium bromide (DODAB) and the neutral polymer poly-oxyethylene (POE) 40 stearate (Journal of Chromatography A 1130 (2006) 265-271). Herein an improved method of generating DODAB/POE stearate coatings is demonstrated, which yields more predictable EOF, more stable coatings, greater average efficiencies and easier method development. In this sequential preparation method the DODAB is first flowed through the capillary, followed by a flow of the POE stearate (sequential method). A tunable EOF (-2.40 to -0.17 x 10⁻⁴ cm²/Vs) is achieved by varying the POE chain length (8, 40 and 100 oxyethylene units). Mixtures of POE 8 and POE 40 stearate enabled continuous variation in EOF from -2.44 to -0.42 x 10⁻⁴ cm²/Vs. Separations of basic proteins yielded efficiencies of 760,000-940,000 plates/m. Coatings formed using the sequential method were more stable over a larger number of runs (%RSD for migration times: 0.7-1.0% over 30 runs) than those formed using the original mixed method (%RSD: 2.4-4.6% over 14 runs). The ability to tune the EOF is important in maximizing the resolution of analytes with similar electrophoretic mobilities. Histone proteins are separated on a sequentially coated capillary with resolution of nine possible subtypes. Acidic proteins are separated on a sequentially coated capillary at pH 6.4.
Department of Chemistry, University of Alberta, Gunning/Lemieux Chemistry Centre, Edmonton, Alberta T6G2G2, Canada.
Amy M
AM
MacDonald
Mahmoud F
MF
Bahnasy
Charles A
CA
Lucy
D000818:Animals; D002417:Cattle; D002645:Chickens; D053841:Electroosmosis; D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000379:methods*; D006657:Histones / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008958:Models, Molecular; D011092:Polyethylene Glycols / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry; D000644:Quaternary Ammonium Compounds / Q000737:chemistry*; D030262:Soybean Proteins / Q000737:chemistry
D006657:Histones; D011506:Proteins; D000644:Quaternary Ammonium Compounds; D030262:Soybean Proteins; C526284:polyoxyethylene 40 stearate; C015831:dimethyldioctadecylammonium; D011092:Polyethylene Glycols