[Effect of Chrysanthemum indicum on ventricular remodeling in rats].
OBJECTIVE
To explore the effects and mechanism of Chrysanthemum indicum on experimental ventricular remodeling induced by isoprenaline (ISO) and L-thyroxine (L-Thy).


METHODS
The ventricular remodeling of mice were induced by subcutaneous injection of ISO with the dosage of 2 mg/kg daily for 7 d and the rats with L-Thy intraperitoneally with the dosage of 0.25 mg/kg daily for 9 d. After 7 days' treatment, the cardiac index and the Ang II content in myocardium of mice were measured. After 9 days' treatment, the ratios of LVW/BW, HW/BW of rats were calculated, the Ang II content in heart tissue and the ALD, TNF-alpha concentration in serun were determined by radioimmunoassay, the Hydroxy proline (Hyp) content in heart tissue were measured by hydrolysis method.


RESULTS
After 7 - 9 days of treatment, Chrysanthemum indicum significantly reduced the left ventricular weight index and heart weight index in mice and rats with myocardial hypertrophy, decreased the content of Ang II in ventricular tissue in mice and rats, and reduced the ALD, TNF-alpha concentration in serum and the Hyp content in ventricular tissue in rats (P < 0.05).


CONCLUSIONS
Chrysanthemum indicum can significantly attenuate the experimental ventricular remodeling; the mechanism may be related with restricting the activity of the sympathetic nervous system and decreasing the levels of Ang II, ALD and TNF-alpha.
Department of Pharmacology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China. zhejiangwuqi@yahoo.com.cn
Qi
Q
Wu
Chang-Xun
CX
Chen
Wei-Liang
WL
Gu
Jian-Ping
JP
Gao
Yin
Y
Wan
Jian
J
Lv
D000450:Aldosterone / Q000097:blood; D000804:Angiotensin II / Q000378:metabolism; D000818:Animals; D031202:Chrysanthemum / Q000737:chemistry*; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D006352:Heart Ventricles / Q000378:metabolism / Q000473:pathology; D017379:Hypertrophy, Left Ventricular / Q000139:chemically induced / Q000188:drug therapy* / Q000503:physiopathology; D007150:Immunohistochemistry; D007545:Isoproterenol / Q000008:administration & dosage; D008297:Male; D051379:Mice; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013974:Thyroxine / Q000008:administration & dosage; D014409:Tumor Necrosis Factor-alpha / Q000097:blood; D016277:Ventricular Function, Left / Q000187:drug effects; D020257:Ventricular Remodeling / Q000187:drug effects*
D004365:Drugs, Chinese Herbal; D014409:Tumor Necrosis Factor-alpha; D000804:Angiotensin II; D000450:Aldosterone; D007545:Isoproterenol; D013974:Thyroxine