Phage display screen for peptides that bind Bcl-2 protein.
Bcl-2 family proteins are key regulators of apoptosis associated with human disease, including cancer. Bcl-2 protein has been found to be overexpressed in many cancer cells. Therefore, Bcl-2 protein is a potential diagnostic target for cancer detection. In the present study, the authors have identified several Bcl-2 binding peptides with high affinity (picomolar range) from a 5-round M13 phage display library screening. These peptides can be used to develop novel diagnostic probes or potent inhibitors with diverse polyvalencies.
Department of Chemistry, College of Natural Sciences, Hanyang University, Seoul, Korea.
Hye-Yeon
HY
Park
Joungmok
J
Kim
June-Haeng
JH
Cho
Ji Young
JY
Moon
Su-Jae
SJ
Lee
Moon-Young
MY
Yoon
D000595:Amino Acid Sequence; D017104:Bacteriophage M13; D001681:Biological Assay; D004926:Escherichia coli; D006367:HeLa Cells; D006801:Humans; D019151:Peptide Library; D010455:Peptides / Q000378:metabolism*; D011485:Protein Binding; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism*
D019151:Peptide Library; D010455:Peptides; D019253:Proto-Oncogene Proteins c-bcl-2