Detection of Mycoplasma pneumoniae in children with lower respiratory tract infections.
Mycoplasma pneumoniae is known to be a major cause of lower respiratory tract infections (LRTIs) in children. We studied 75 children who had been hospitalized for community-acquired LRTIs for the detection of M. pneumoniae by serological analysis and polymerase chain reaction (PCR) to amplify a 277-base pair region of 16S rDNA gene of M. pneumoniae applied to throat swab specimens. Serological and/or PCR positive results diagnosed M. pneumoniae infection in 23 (30.7%) patients.
Department of Microbiology, Maulana Azad Medical College, New Delhi-110002, India.
Megha
M
Maheshwari
Surinder
S
Kumar
G R
GR
Sethi
P
P
Bhalla
D000907:Antibodies, Bacterial / Q000097:blood; D002648:Child; D002675:Child, Preschool; D017714:Community-Acquired Infections / Q000175:diagnosis / Q000453:epidemiology / Q000382:microbiology; D005260:Female; D006801:Humans; D007194:India / Q000453:epidemiology; D007223:Infant; D008297:Male; D009177:Mycoplasma pneumoniae / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D010614:Pharynx / Q000382:microbiology; D011019:Pneumonia, Mycoplasma / Q000175:diagnosis* / Q000453:epidemiology / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods; D015995:Prevalence; D012141:Respiratory Tract Infections / Q000175:diagnosis* / Q000453:epidemiology / Q000382:microbiology*
D000907:Antibodies, Bacterial