The effects of concentration-dependent morphology of self-assembling RADA16 nanoscaffolds on mixed retinal cultures.
RADA16 self-assembling peptide nanofiber scaffolds (SAPNSs) have been shown to have positive effects on neural regeneration following injury to the central nervous system in vivo, but mechanisms are unclear. Here we show that RADA16 SAPNSs form scaffolds of increasing fiber density with increasing peptide concentration which in turn has a concentration-dependent effect on neurons and astrocytes in mixed retinal cultures. Importantly, we report that the final nanoscale fiber architecture is an important factor to consider in designing scaffolds to promote regeneration in the central nervous system.
School of Biomedical, Biomolecular and Chemical Sciences, The University of Western Australia, Crawley, WA 6009, Australia.
Dominic
D
Ho
Melinda
M
Fitzgerald
Carole A
CA
Bartlett
Bogdan
B
Zdyrko
Igor A
IA
Luzinov
Sarah A
SA
Dunlop
K
K
Swaminathan Iyer
D003460:Crystallization / Q000379:methods*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D008422:Materials Testing; D008968:Molecular Conformation; D046912:Multiprotein Complexes / Q000737:chemistry; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure*; D036103:Nanotechnology / Q000379:methods; D010316:Particle Size; D010455:Peptides / Q000737:chemistry*; D013499:Surface Properties; D054457:Tissue Scaffolds
D046912:Multiprotein Complexes; D010455:Peptides; C546681:RADA16-I