The role of mast cells in atrial natriuretic peptide-induced cutaneous inflammation.
Atrial natriuretic peptide (ANP) is widely distributed throughout the heart, skin, gastrointestinal and genital tracts, and nervous and immune systems. ANP acts to mediate vasodilation and induces mast cell activation in both human and rats in vitro. However, the mechanisms of ANP-induced mast cell activation, the extent to which ANP can induce tissue swelling, mast cell degranulation, and granulocyte infiltration in mouse skin are not fully understood. This issue was investigated by treatment with ANP in rat peritoneal mast cells (RPMCs) and mouse peritoneal mast cells (MPMCs) in vitro and by injection of ANP into the skin of congenic normal WBB6F1/J-Kit+/Kit+ +/+, genetically mast cell-deficient WBB6F1/J-Kit(W)/Kit(W-v) (W/W(v)) and mast cell-engrafted W/W(v) (BMCMC→W/W(v)) mice in vivo. ANP induced the release of histamine and TNF-α from RPMCs and enhanced serotonin release from MPMCs, in a dose-dependent fashion, as well as reduced cAMP level of RPMCs in vitro. In +/+ mice, ANP induced significant tissue swelling, mast cell degranulation, and granulocyte infiltration in a dose-dependent manner, whereas not in genetically mast cell-deficient W/W(v) mice. However, ANP-induced cutaneous inflammation has been restored in BMCMC→W/W(v) mice. These data indicate that mast cells play a key role in the ANP-induced cutaneous inflammation.
Department of Anatomy, Chonbuk National University Medical School, Jeonju, Jeonbuk 561-756, Republic of Korea.
Ok Hee
OH
Chai
Eui-Hyeog
EH
Han
Yun Ho
YH
Choi
Suhn Hee
SH
Kim
Hyoung Tae
HT
Kim
Stephen J
SJ
Galli
Chang Ho
CH
Song
D000279:Administration, Cutaneous; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000378:metabolism / Q000494:pharmacology; D015550:Cell Degranulation / Q000187:drug effects / Q000276:immunology; D004306:Dose-Response Relationship, Immunologic; D006098:Granulocytes / Q000166:cytology / Q000276:immunology; D006632:Histamine / Q000096:biosynthesis; D006801:Humans; D007249:Inflammation / Q000276:immunology* / Q000378:metabolism; D008297:Male; D008407:Mast Cells / Q000166:cytology* / Q000276:immunology* / Q000378:metabolism / Q000637:transplantation; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D020556:Neutrophil Infiltration / Q000187:drug effects; D010537:Peritoneum / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D051381:Rats; D012701:Serotonin / Q000096:biosynthesis; D012867:Skin / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D019737:Transplants; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis* / Q000378:metabolism; D014664:Vasodilation / Q000502:physiology
D014409:Tumor Necrosis Factor-alpha; D012701:Serotonin; D006632:Histamine; D009320:Atrial Natriuretic Factor