Comparison of proteomic biomarker panels in urine and serum for ovarian cancer diagnosis.
OBJECTIVE
The purposes of this study were to confirm previously found candidate epithelial ovarian cancer biomarkers in urine and to compare a paired serum biomarker panel and a urine biomarker panel from the same study cohort with regard to the receiver operating characteristic curve (ROC) area under the ROC curve (AUC) values.


METHODS
Four significant urine biomarkers were confirmed among 130 pelvic mass patients in the present study. The four biomarkers form a potential urine biomarker panel. From the same study cohort, the potential urine biomarker panel was compared to a serum biomarker panel, consisting of seven proteins/peptides, OvaRI.


RESULTS
Multivariate analysis of the urine panel demonstrated a significant differentiation (p<0.0001) between epithelial ovarian cancer patients and patients with benign ovarian pelvic masses. The ROC AUC of the urine panel was 0.84 and the ROC AUC of OvaRI was 0.83. Combining the urine panel with OvaRI demonstrated a significant contribution from both, for urine peaks, OR=2.12 and for OvaRI, OR=1.39; the ROC AUC of this model was 0.88.


CONCLUSIONS
We demonstrated that both urine and serum can be used individually or in combination to potentially aid in ovarian cancer diagnostics. Urine proteomic profiling could provide biomarkers for the non-invasive test required in clinical practice.
Department of Gynaecology, Rigshospitalet, Copenhagen University Hospital, Copenhagen, Denmark. alpetri@dadlnet.dk
Anette Lykke
AL
Petri
Anja Hviid
AH
Simonsen
Estrid
E
Høgdall
Ib Jarle
IJ
Christensen
Susanne Krüger
SK
Kjaer
Christine
C
Yip
Signe
S
Risum
Anette Tønnes
AT
Pedersen
Dorte
D
Hartwell
Eric T
ET
Fung
Claus
C
Høgdall
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000097:blood* / Q000652:urine*; D000077216:Carcinoma, Ovarian Epithelial; D005260:Female; D006801:Humans; D008875:Middle Aged; D009375:Neoplasms, Glandular and Epithelial / Q000097:blood / Q000175:diagnosis / Q000378:metabolism / Q000652:urine; D010051:Ovarian Neoplasms / Q000097:blood / Q000175:diagnosis / Q000378:metabolism / Q000652:urine; D040901:Proteomics / Q000379:methods*; D053719:Tandem Mass Spectrometry; D055815:Young Adult
D014408:Biomarkers, Tumor