Designing carbohydrate nanoparticles for prolonged efficacy of antimicrobial peptide.
In this work, carbohydrate nanoparticles were created to prolong the efficacy of antimicrobial peptide against pathogens. Nisin and Listeria monocytogenes were used as the peptide and pathogen models, respectively, and phytoglycogen (PG)-based nanoparticles were developed as carriers of nisin. PG from su1 mutant maize was subjected to β-amylolysis as well as subsequent succinate or octenyl succinate substitutions. The goal was to minimize the loss of peptide during storage and meanwhile realize an effective release in the presence of bacteria. The capabilities of PG derivatives as carriers of nisin were evaluated using centrifugal ultrafiltration, zeta-potential, and the initial availability of nisin against L. monocytogenes. All methods indicated that nisin loading was favored by a high degree of substitution (DS), presence of hydrophobic octenyl moiety, and β-amylolysis of PG nanoparticles. To evaluate the prolonged nisin efficacy, preparations containing nisin and PG derivatives were loaded into a BHI-agar deep-well model (mimicking nisin depletion at the nutrient-containing surface). The residual inhibitory activities of preparations against L. monocytogenes were monitored during 21 days of storage at 4 °C. The results showed that all PG derivatives led to the prolonged retention of nisin activity and the longest retention was associated with high DS, β-amylolysis, and octenyl succinate. Evidently, both electrostatic and hydrophobic interactions are the driving forces of nisin adsorption, and the glucan structure at the nanoparticle surface also affects nisin loading and retention during storage.
Department of Food Science, Purdue University, 745 Agriculture Mall Drive, West Lafayette, IN 47907, United States.
Lin
L
Bi
Lei
L
Yang
Ganesan
G
Narsimhan
Arun K
AK
Bhunia
Yuan
Y
Yao
D023181:Antimicrobial Cationic Peptides / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics* / Q000494:pharmacology; D001682:Biological Availability; D002241:Carbohydrates / Q000737:chemistry*; D049109:Cell Proliferation / Q000187:drug effects; D003692:Delayed-Action Preparations / Q000138:chemical synthesis / Q000737:chemistry; D003912:Dextrins / Q000737:chemistry / Q000378:metabolism; D004337:Drug Carriers / Q000138:chemical synthesis / Q000737:chemistry*; D004355:Drug Stability; D005936:Glucans / Q000737:chemistry / Q000378:metabolism; D008089:Listeria monocytogenes / Q000187:drug effects; D046529:Microscopy, Electron, Transmission; D008970:Molecular Weight; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D009561:Nisin / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D010316:Particle Size; D013046:Specific Gravity; D055672:Static Electricity; D013386:Succinates / Q000737:chemistry; D019802:Succinic Acid / Q000737:chemistry; D013387:Succinic Anhydrides / Q000737:chemistry; D013499:Surface Properties; D003313:Zea mays / Q000737:chemistry; D001614:beta-Amylase / Q000378:metabolism
D023181:Antimicrobial Cationic Peptides; D002241:Carbohydrates; D003692:Delayed-Action Preparations; D003912:Dextrins; D004337:Drug Carriers; D005936:Glucans; D013386:Succinates; D013387:Succinic Anhydrides; D009561:Nisin; C028480:octenyl succinate; D019802:Succinic Acid; D001614:beta-Amylase