[Comparison between MLO-Y4 osteocyte and osteoblast to support osteoclast formation in vitro].
OBJECTIVE
To compare between MLO-Y4 osteocyte and osteoblast to support osteoclast formation in co-culture system.


METHODS
MLO-Y4 cells and murine osteoblast cells were co-cultured with bone marrow cells with or without vitamin D₃ presence.Bone marrow cells were as control group. Tartrat resistant acid phosphatase (TRAP)+ giant cells with three or more nuclei were counted and compared under a microscope at day 9.


RESULTS
In the absence of vitamin D₃, (1963.3 ± 93.1)/plate osteoclasts were observed when MLO-Y4 cells co-cultured with bone marrow cells in 24-well plate.While only (12.7 ± 5.5)/plate osteoclasts were found in the osteoblast group, and (6.0 ± 1.0)/plate in control group. The statistical difference occurs for any two groups (P < 0.05). Vitamin D₃ could significantly increase osteoclast formation in the three groups.


CONCLUSIONS
Osteocytes could induce osteoclastogenesis without the presence of vitamin D₃ and vitamin D₃ could enhance the induction effects of MLO-Y4 and osteoblast cells.
Department of Stomatology, Peking Union Medical College Hospital, Peking Union Medical College, Beijing, China.
Liang
L
Cui
Xiao-tong
XT
Li
Yan-qi
YQ
Yang
Min-kui
MK
Fu
Ding
D
Zhang
D000818:Animals; D002460:Cell Line; D002762:Cholecalciferol / Q000737:chemistry; D018920:Coculture Techniques; D003470:Culture Media / Q000737:chemistry; D051379:Mice; D010006:Osteoblasts / Q000166:cytology*; D010010:Osteoclasts / Q000166:cytology*; D010011:Osteocytes / Q000166:cytology*
D003470:Culture Media; D002762:Cholecalciferol