Effects of rotenone and other mitochondrial complex I inhibitors on the brine shrimp Artemia.
(Artemia) nauplii was used to asses the toxicity of rotenone, MPTP (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine), MP+ (1-methyl-4-phenylpyridinium) and the effect of L-DOPA co-treatment with rotenone. Rotenone had a dose dependent effect on mortality (LC₅₀: 0.37 ± 0.04 μM mean ± S E, n = 24), while MPTP and MP+ proved to be toxic in millimolar range (LC₅₀: 0.21 ± 0.09 mM and 0.20 ± 0.08 mM, respectively, n = 4). L-DOPA (50-200 μM) co-treatment increased the survival of the rotenone-treated animals (LC₅₀: 0.51 ± 0.23 μM, 1.03 ± 0.66 μM, and 0.76 ± 0.52 μM, respectively). In the whole body tissue homogenates of Artemia, sublethal (up to 0.3 μM) concentrations of rotenone increased the glutathione S-transferase (GST) activity by up to 50 about percent (LC₅₀: 53.3 ± 6.8 nM/min/mg protein, against 34.7 ± 3.6 nM/min/mg protein, n = 4). Nauplii treated in 100 mM L-DOPA and rotenone together showed further increase of GST activity all across the range of rotenone concentrations. These results on Artemia nauplii show similarities with other animal models, when complex I inhibitors were tested. Biochemical measurements suggest a protective role of L-DOPA by increasing the GST activity as part of the intracellular defences during toxin-evoked oxidative stress.
Hungarian Academy of Sciences, Balaton Limnological Research Institute, Tihany, Hungary. agnes@tres.blki.hu
Agnes
A
Vehovszky
Henriette
H
Szabó
A
A
Acs
J
J
Gyori
Anna
A
Farkas
D015632:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine / Q000494:pharmacology; D015655:1-Methyl-4-phenylpyridinium / Q000494:pharmacology; D000818:Animals; D001156:Artemia / Q000187:drug effects* / Q000378:metabolism; D001681:Biological Assay; D001711:Biotransformation; D042967:Electron Transport Complex I / Q000037:antagonists & inhibitors*; D005982:Glutathione Transferase / Q000737:chemistry / Q000378:metabolism; D006540:Herbicides / Q000494:pharmacology; D008658:Inactivation, Metabolic; D007980:Levodopa / Q000494:pharmacology; D012402:Rotenone / Q000494:pharmacology*
D006540:Herbicides; D012402:Rotenone; D007980:Levodopa; D015632:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine; D005982:Glutathione Transferase; D042967:Electron Transport Complex I; D015655:1-Methyl-4-phenylpyridinium