Ultrasonic differentiation of normal versus malignant breast epithelial cells in monolayer cultures.
Normal and malignant mammary epithelial cells were studied using laboratory measurements, wavelet analysis, and numerical simulations of monolayer cell cultures to determine whether microscopic breast cancer can be detected in vitro with high-frequency ultrasound. Pulse-echo waveforms were acquired by immersing a broadband, unfocused 50-MHz transducer in the growth media of cell culture well plates and collecting the first reflection from the well bottoms. The simulations included a multilayer pulse-reflection model and a model of two-dimensional arrays of spherical cells and nuclei. The results show that normal and malignant cells produce time-domain signals and spectral features that are significantly different.
Department of Physics, Utah State University, Logan, Utah 84322, USA. tim.doyle@usu.edu
Timothy E
TE
Doyle
Jeffrey B
JB
Goodrich
Brady J
BJ
Ambrose
Hemang
H
Patel
Soonjo
S
Kwon
Lee H
LH
Pearson
D001943:Breast Neoplasms / Q000000981:diagnostic imaging* / Q000473:pathology; D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D004847:Epithelial Cells / Q000000981:diagnostic imaging* / Q000473:pathology; D005260:Female; D006801:Humans; D014465:Ultrasonics / Q000295:instrumentation / Q000379:methods*; D014463:Ultrasonography; D058067:Wavelet Analysis