Renal epithelial cell injury and its promoting role in formation of calcium oxalate monohydrate.
The injurious effect of hydrogen peroxide (H(2)O(2)) on renal epithelial cells of the African green monkey (Vero cells) and the difference in the modulation of Vero cells on crystal growth of calcium oxalate (CaOxa) before and after injury were investigated. The degree of injury of Vero cells was proportional to the concentration and action time of H(2)O(2). After the cells had been injured, the released amount of malonaldehyde in the culture medium increased, the superoxide dismutase activity decreased, the expression quantity of osteopontin on the surface of Vero cells increased significantly, the zeta potential became more negative, and the amount of CaOxa crystals adhering to cells increased. The CaOxa crystals induced by the cells in the control group were round and blunt; however, those induced by the injured cells had irregular shapes with sharp edges and corners. As the crystallization time increased from 6 to 24 h, the size of the crystals induced by the injured cells increased accordingly, whereas that of crystals induced by the control cells did not increase significantly. The injured cells could promote the growth of CaOxa crystals and their adhesion to the cells; thus, the formation of CaOxa stones was promoted. The cells in the control group could also be injured after being incubated with supersaturated CaOxa solution for a long time, which promoted the crystallization of CaOxa. The results suggest that the retention of supersaturated CaOxa solution or CaOxa crystals in the urinary tract for a long time is a risk factor for the formation of kidney stones.
Department of Chemistry, Jinan University, Guangzhou, 510632, China. toyjm@jnu.edu.cn
Jian-Ming
JM
Ouyang
Xiu-Qiong
XQ
Yao
Jin
J
Tan
Feng-Xin
FX
Wang
D000818:Animals; D002129:Calcium Oxalate / Q000737:chemistry* / Q000378:metabolism*; D002470:Cell Survival / Q000187:drug effects; D002522:Chlorocebus aethiops; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007668:Kidney / Q000166:cytology*; D008315:Malondialdehyde / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D053495:Osteopontin / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D014709:Vero Cells; D014961:X-Ray Diffraction
D053495:Osteopontin; D002129:Calcium Oxalate; D008315:Malondialdehyde; D006861:Hydrogen Peroxide; D013482:Superoxide Dismutase