Hypoxia-induced retinopathy model in adult zebrafish.
Hypoxia-induced vascular responses, including angiogenesis, vascular remodeling and vascular leakage, significantly contribute to the onset, development and progression of retinopathy. However, until recently there were no appropriate animal disease models recapitulating adult retinopathy available. In this article, we describe protocols that create hypoxia-induced retinopathy in adult zebrafish. Adult fli1:EGFP zebrafish are placed in hypoxic water for 3-10 d and retinal neovascularization is analyzed using confocal microscopy. It usually takes 11 d to obtain conclusive results using the hypoxia-induced retinopathy model in adult zebrafish. This model provides a unique opportunity to study kinetically the development of retinopathy in adult animals using noninvasive protocols and to assess therapeutic efficacy of orally active antiangiogenic drugs.
Institute of Medicine and Health, Linkoping University, Linkoping, Sweden.
Ziquan
Z
Cao
Lasse D
LD
Jensen
Pegah
P
Rouhi
Kayoko
K
Hosaka
Toste
T
Länne
John F
JF
Steffensen
Erik
E
Wahlberg
Yihai
Y
Cao
D000818:Animals; D003930:Diabetic Retinopathy / Q000209:etiology*; D004195:Disease Models, Animal; D049452:Green Fluorescent Proteins / Q000378:metabolism; D000860:Hypoxia / Q000150:complications*; D008856:Microscopy, Fluorescence; D009389:Neovascularization, Pathologic / Q000209:etiology / Q000473:pathology*; D015027:Zebrafish
C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins