Bio-inspired porous SiC ceramics loaded with vancomycin for preventing MRSA infections.
Implant-related infections are a serious complication in orthopaedic and dental surgery resulting in prolonged hospitalization, high medical costs and patient mortality. The development of porous implants loaded with antibiotics may enable a local delivery for preventing surface colonization and biofilm formation. A new generation of bio-derived porous ceramic material that mimics hierarchical structures from Nature was evaluated. Silicon carbide ceramics derived from Sapelli wood (bioSiC) were obtained by pyrolysis of Entandrophragma cylindricum wood followed by infiltration with molten silicon. This process renders disks that keep the bimodal pore size distribution (3 and 85 μm) of the original material and are highly cytocompatible (BALB/3T3 cell line). The ability of the bio-ceramic to load the antimicrobial agent vancomycin was evaluated by immersion of disks in drug solutions covering a wide range of concentrations. The disks released at pH 7.4 an important amount of drug during the first 2 h (up to 11 mg/g bioSiC) followed by a slower release, which is related to the presence of macro- and mesopores. Finally, the anti-biofilm effect against methicillin resistant Staphylococcus aureus was assessed and a considerable reduction (92%) of the bacterial film was observed. Results highlight the bioSiC potential as component of medicated medical devices.
Dpto. Farmacia y Tecnología Farmacéutica, Facultad de Farmacia, Universidad de Santiago de Compostela, 15782, Santiago de Compostela, Spain.
P
P
Díaz-Rodríguez
M
M
Landin
A
A
Rey-Rico
J
J
Couceiro
T
T
Coenye
P
P
González
J
J
Serra
M
M
López-Álvarez
B
B
León
D016475:3T3 Cells; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D018441:Biofilms; D017554:Carbon Compounds, Inorganic / Q000737:chemistry; D002470:Cell Survival; D002516:Ceramics; D004305:Dose-Response Relationship, Drug; D005452:Fluoresceins / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D055624:Methicillin-Resistant Staphylococcus aureus / Q000378:metabolism*; D051379:Mice; D016459:Prosthesis-Related Infections / Q000517:prevention & control*; D017655:Silicon Compounds / Q000737:chemistry; D013997:Time Factors; D014640:Vancomycin / Q000627:therapeutic use*
D001672:Biocompatible Materials; D017554:Carbon Compounds, Inorganic; D005452:Fluoresceins; D017655:Silicon Compounds; D014640:Vancomycin; C007740:fluorexon; C022088:silicon carbide