RNA decay modulates gene expression and controls its fidelity.
Maintenance of cellular function relies on the expression of genetic information with high fidelity, a process in which RNA molecules form an important link. mRNAs are intermediates that define the proteome, rRNAs and tRNAs are effector molecules that act together to decode mRNA sequence information, and small noncoding RNAs can regulate mRNA half-life and translatability. The steady-state levels of these RNAs occur through transcriptional and posttranscriptional regulatory mechanisms, of which RNA decay pathways are integral components. RNA decay can initiate from the ends of a transcript or through endonucleolytic cleavage, and numerous factors that catalyze or promote these reactions have been identified and characterized. The rate at which decay occurs depends on RNA sequence or structural elements and usually requires the RNA to be modified in a way that allows recruitment of the decay machinery to the transcript through the binding of accessory factors or small RNAs. The major RNA decay pathways also play important roles in the quality control (QC) of gene expression. Acting in both the nucleus and cytoplasm, multiple QC factors monitor newly synthesized transcripts, or mRNAs undergoing translation, for properties essential to function, including structural integrity or the presence of complete open-reading frames. Transcripts targeted by these surveillance mechanisms are rapidly shunted into conventional decay pathways where they are degraded rapidly to ensure that they do not interfere with the normal course of gene expression. Collectively, degradative mechanisms are important determinants of the extent of gene expression and play key roles in maintaining its accuracy.
Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, MA 01655-0122, USA.
Shubhendu
S
Ghosh
Allan
A
Jacobson
D000818:Animals; D015870:Gene Expression / Q000235:genetics*; D006801:Humans; D008954:Models, Biological; D011485:Protein Binding / Q000502:physiology; D014176:Protein Biosynthesis / Q000235:genetics* / Q000502:physiology; D011786:Quality Control; D020871:RNA Stability / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000378:metabolism; D014158:Transcription, Genetic / Q000235:genetics / Q000502:physiology
D012333:RNA, Messenger; D016601:RNA-Binding Proteins