Mesenchymal cells of the decidual tooth pulp: cytophenotype and initial evaluation of possibility of their use in bone tissue engineering.
Cultures of mesenchymal cells from human decidual tooth pulp were derived. The phenotype and capacity to osteogenic and adipogenic differentiation of these cells are close to those of bone marrow mesenchymal stem cells. Decidual tooth pulp mesenchymal cells populate biodegraded polylactide scaffolds and hence, can be used for the creation of tissue engineering transplants for bone defect repair. Storage of decidual tooth pulp mesenchymal cells in the stem cell cryobanks together with umbilical blood will appreciably extent the periods of age for collection of juvenile autologous stem cells for use throughout the life span.
V. N. Orekhovich Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Moscow, Russia. vakhrunya@gmail.com
I V
IV
Vakhrushev
Yu G
YG
Suzdaltseva
V V
VV
Burunova
P A
PA
Karalkin
A Yu
AY
Lupatov
K N
KN
Yarygin
D001842:Bone and Bones; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation / Q000502:physiology; D003782:Dental Pulp / Q000166:cytology*; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008856:Microscopy, Fluorescence; D011091:Polyesters; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds; D014094:Tooth, Deciduous / Q000166:cytology*
D011091:Polyesters; C033616:poly(lactide)