An effective gold nanotubes electrode for amperometric biosensor.
A sensitive and effective amperometric glucose biosensor based on gold nanotubes electrode (GNTE) was investigated. Gold nanotubes (GNTs), which were prepared by electroless plating of the metal within the pores of nanoporous polycarbonate (PC) track-etched membranes, were filled into a hollow teflon cylinder to construct a GNTE. Glucose oxidase (GOD) was immobilized on the electrode via glutaraldehyde cross-linkage method. The electrochemical properties were investigated by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The km value of the immobilized glucose oxidase on GNTE was 0.47 mM. The biosensor showed a linear range from 0.4 to 11 mM with excellent sensitivity of 8.77 microA cm(-2) mM(-1) and fast response time within 5 s.
Shanghai Institute of Ceramics, Chinese Academy of Sciences, Key Lab of Inorganic Coating Materials, Shanghai 200050, P.R. China.
Yunli
Y
Wang
Yingchun
Y
Zhu
Yanyan
Y
Liu
Yu
Y
Yang
Qichao
Q
Ruan
Fangfang
F
Xu
D015374:Biosensing Techniques / Q000379:methods*; D058266:Dielectric Spectroscopy; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D005947:Glucose / Q000032:analysis; D005949:Glucose Oxidase / Q000737:chemistry / Q000378:metabolism; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D058726:Nanoconjugates / Q000737:chemistry* / Q000648:ultrastructure; D043942:Nanotubes / Q000737:chemistry* / Q000648:ultrastructure
D004800:Enzymes, Immobilized; D058726:Nanoconjugates; D006046:Gold; D005949:Glucose Oxidase; D005947:Glucose