Irrelevance of CHEK2 variants to diagnosis of breast/ovarian cancer predisposition in Polish cohort.
CHEK2 gen encodes cell cycle checkpoint kinase 2 that participates in the DNA repair pathway, cell cycle regulation and apoptosis. Mutations in CHEK2 gene may result in kinase inactivation or reduce both catalytic activity and capability of binding other proteins. Some studies indicate that alterations in CHEK2 gene confers increase the risk of breast cancer and some other malignancies, while the results of other studies are inconclusive. Thus the significance of CHEK2 mutations in aetiology of breast cancer is still debatable. The aim of our study was to evaluate the relationship between the breast/ovarian cancer and CHEK2 variants by: i) the analysis of the frequency of selected CHEK2 variants in breast and ovarian cancer patients compared to the controls; ii) evaluation of relationships between the certain CHEK2 variants and clinico-histopathological and pedigree data. The study was performed on 284 breast cancer patients, 113 ovarian cancer patients and 287 healthy women. We revealed the presence of 430T > C, del5395 and IVS2 + 1G > A variants but not 1100delC in individuals from both study and control groups. We did not observe significant differences between cancer patients and controls neither in regard to the frequency nor to the type of CHEK2 variants. We discussed the potential application of CHEK2 variants in the evaluation of breast and ovarian cancer predisposition.
Department of Genetics, University of Rzeszow, Rejtana 16 C, 35-959, Rzeszow, Poland. amyszka@tlen.pl
Aleksander
A
Myszka
Pawel
P
Karpinski
Ryszard
R
Slezak
Halina
H
Czemarmazowicz
Agnieszka
A
Stembalska
Justyna
J
Gil
Izabela
I
Laczmanska
Damian
D
Bednarczyk
Elzbieta
E
Szmida
Maria Malgorzata
MM
Sasiadek
D000328:Adult; D000368:Aged; D001943:Breast Neoplasms / Q000175:diagnosis / Q000453:epidemiology / Q000235:genetics*; D016022:Case-Control Studies; D064447:Checkpoint Kinase 2; D004252:DNA Mutational Analysis; D005260:Female; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006801:Humans; D008875:Middle Aged; D016017:Odds Ratio; D010051:Ovarian Neoplasms / Q000175:diagnosis / Q000453:epidemiology / Q000235:genetics*; D017354:Point Mutation; D011044:Poland / Q000453:epidemiology; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D017384:Sequence Deletion
D064447:Checkpoint Kinase 2; C578634:CHEK2 protein, human; D017346:Protein Serine-Threonine Kinases