Protective effects of Lactobacillus plantarum against epithelial barrier dysfunction of human colon cell line NCM460.
OBJECTIVE
To investigate the effects of Lactobacillus plantarum (L. plantarum) in the intestinal permeability and expression of tight junction (TJ) using the normal human colon cell line NCM460.


METHODS
Paracellular permeability of NCM460 monolayers was determined by transepithelial electrical resistance and dextran permeability. Expression of TJ proteins in NCM460 cell monolayers was detected by Western blotting and quantitative real-time polymerase chain reaction.


RESULTS
L. plantarum played an important role in increasing transepithelial electrical resistance and decreasing the permeability to macromolecules of NCM460 monolayers against the disruption caused by enteropathogenic Escherichia coli (E. coli) or enteroinvasive E. coli. L. plantarum also prevented the decrease in the expression of TJ proteins and F-actin in NCM460 cells.


CONCLUSIONS
L. plantarum can protect against dysfunction of NCM460 intestinal epithelial barrier caused by enteropathogenic E. coli or enteroinvasive E. coli, and thus can be a potential candidate of therapeutic agents for the treatment of intestinal diseases.
Department of Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, 600 Yishan Road, Shanghai 200233, China.
Zhi-Hua
ZH
Liu
Tong-Yi
TY
Shen
Peng
P
Zhang
Yan-Lei
YL
Ma
Mary Pat
MP
Moyer
Huan-Long
HL
Qin
D000199:Actins / Q000378:metabolism; D015153:Blotting, Western; D002460:Cell Line; D003106:Colon / Q000166:cytology / Q000378:metabolism / Q000382:microbiology*; D003911:Dextrans / Q000378:metabolism; D017097:Electric Impedance; D054308:Enteropathogenic Escherichia coli / Q000472:pathogenicity; D004926:Escherichia coli / Q000472:pathogenicity*; D006801:Humans; D048191:Lactiplantibacillus plantarum / Q000502:physiology*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D010539:Permeability; D019936:Probiotics; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019108:Tight Junctions / Q000378:metabolism / Q000382:microbiology*; D013997:Time Factors
D000199:Actins; D003911:Dextrans; D008565:Membrane Proteins; D012333:RNA, Messenger