[Evaluating of influence of repeated thaw/freeze cycles on IgG and IgM stability].
The purpose of this study was evaluating influence of repeated cycles of thawing and freezing serum samples on IgG and IgM stability. Serum positive samples for anti-cytomegalovirus and antimeasles virus IgG and/or IgM were aliquoted. Tubes containing 100 microl aliquot were frozen at -20 degrees C. Samples were repetitively thawed and froze in one to ten cycles. Antibodies presence was examined with commercially available ELISA tests. All samples reminded positive even after ten repeated thaw/freeze cycles.
Zakład Wirusologii NIZP-PZH w Warszawie.
Joanna
J
Siennicka
Anna
A
Laskowska
Agnieszka
A
Trzcińska
D003587:Cytomegalovirus / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005615:Freezing; D007074:Immunoglobulin G / Q000302:isolation & purification*; D007075:Immunoglobulin M / Q000302:isolation & purification*; D018103:Morbillivirus / Q000276:immunology*; D055550:Protein Stability
D007074:Immunoglobulin G; D007075:Immunoglobulin M