Maintenance of undifferentiated state of human embryonic stem cells in chemical defined medium at high clone density without exogenous cell factors.
OBJECTIVE
To establish human embryonic stem cells (hESCs) feeder-independent and cell factor-free culture system.


METHODS
Effect of high and low clone densities of hESCs culture was compared and impact of the clone densities to hESCs culture media was analyzed.


RESULTS
HESCs could maintain their undifferentiated states at high clone density (34 clones/cm²) without cell factors. At the same time, the bone morphology protein (BMP)-like induction of N2 and B27 supplements (NB) medium could be modulated by the clone density, and high level of BMP-like induction was accompanied by high clone density.


CONCLUSIONS
High clone density of hESCs can change the environments by themselves to maintain the undifferentiated states, which provides a new clue to explore the mechanism of undifferentiated states of hESCs and simplify the culture medium.
Institute of Reproduction and Stem Cell Engineering, Central South University, Changsha 410078, China.
Shuwei
S
Luo
Ge
G
Lin
Zhen
Z
Sun
Pingyuan
P
Xie
Tiancheng
T
Liu
Guangxiu
G
Lu
D019485:Bone Morphogenetic Proteins / Q000494:pharmacology; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation / Q000502:physiology*; D003001:Cloning, Molecular; D003470:Culture Media; D053595:Embryonic Stem Cells / Q000166:cytology*; D018507:Gene Expression Regulation, Developmental; D006801:Humans
D019485:Bone Morphogenetic Proteins; D003470:Culture Media