Green propolis phenolic compounds act as vaccine adjuvants, improving humoral and cellular responses in mice inoculated with inactivated vaccines.
Adjuvants play an important role in vaccine formulations by increasing their immunogenicity. In this study, the phenolic compound-rich J fraction (JFR) of a Brazilian green propolis methanolic extract stimulated cellular and humoral immune responses when co-administered with an inactivated vaccine against swine herpesvirus type 1 (SuHV-1). When compared to control vaccines that used aluminium hydroxide as an adjuvant, the use of 10 mg/dose of JFR significantly increased (p < 0.05) neutralizing antibody titres against SuHV-1, as well as the percentage of protected animals following SuHV-1 challenge (p < 0.01). Furthermore, addition of phenolic compounds potentiated the performance of the control vaccine, leading to increased cellular and humoral immune responses and enhanced protection of animals after SuHV-1 challenge (p < 0.05). Prenylated compounds such as Artepillin C that are found in large quantities in JFR are likely to be the substances that are responsible for the adjuvant activity.
Centro de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brasil. geferson.fischer@gmail.com
Geferson
G
Fischer
Niraldo
N
Paulino
Maria C
MC
Marcucci
Bianca S
BS
Siedler
Lívia S
LS
Munhoz
Paula F
PF
Finger
Gilberto D
GD
Vargas
Sílvia O
SO
Hübner
Telmo
T
Vidor
Paulo M
PM
Roehe
D000276:Adjuvants, Immunologic; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology*; D011558:Herpesvirus 1, Suid / Q000276:immunology*; D022283:Herpesvirus Vaccines / Q000276:immunology*; D007111:Immunity, Cellular / Q000276:immunology; D056724:Immunity, Humoral / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D011429:Propolis / Q000737:chemistry*; D011557:Pseudorabies / Q000276:immunology / Q000517:prevention & control*; D013552:Swine; D015164:Vaccines, Inactivated / Q000276:immunology
D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D022283:Herpesvirus Vaccines; D015164:Vaccines, Inactivated; D011429:Propolis