Vernalization-mediated epigenetic silencing by a long intronic noncoding RNA.
Vernalization is an environmentally-induced epigenetic switch in which winter cold triggers epigenetic silencing of floral repressors and thus provides competence to flower in spring. In Arabidopsis, winter cold triggers enrichment of tri-methylated histone H3 Lys(27) at chromatin of the floral repressor, FLOWERING LOCUS C (FLC), and results in epigenetically stable repression of FLC. This epigenetic change is mediated by an evolutionarily conserved repressive complex, polycomb repressive complex 2 (PRC2). Here, we show that a long intronic noncoding RNA [termed COLD ASSISTED INTRONIC NONCODING RNA (COLDAIR)] is required for the vernalization-mediated epigenetic repression of FLC. COLDAIR physically associates with a component of PRC2 and targets PRC2 to FLC. Our results show that COLDAIR is required for establishing stable repressive chromatin at FLC through its interaction with PRC2.
Section of Molecular Cell and Developmental Biology and Institute for Cellular and Molecular Biology, the University of Texas at Austin, TX 78712, USA.
Jae Bok
JB
Heo
Sibum
S
Sung
D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D002352:Carrier Proteins / Q000378:metabolism; D002843:Chromatin / Q000378:metabolism; D003080:Cold Temperature; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D044127:Epigenesis, Genetic; D035264:Flowers / Q000254:growth & development; D018506:Gene Expression Regulation, Plant; D020868:Gene Silencing; D017343:Genes, Plant; D006657:Histones / Q000378:metabolism; D018398:Homeodomain Proteins / Q000378:metabolism; D007438:Introns; D026161:MADS Domain Proteins / Q000235:genetics* / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism; D030821:Plants, Genetically Modified; D063146:Polycomb-Group Proteins; D011401:Promoter Regions, Genetic; D034622:RNA Interference; D012319:RNA Polymerase II / Q000378:metabolism; D016372:RNA, Antisense / Q000235:genetics / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D022661:RNA, Untranslated / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D012097:Repressor Proteins / Q000378:metabolism; D020218:Response Elements; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic
D029681:Arabidopsis Proteins; C104597:CURLY LEAF protein, Arabidopsis; D002352:Carrier Proteins; D002843:Chromatin; D004268:DNA-Binding Proteins; C418902:At5g10140 protein, Arabidopsis; D006657:Histones; D018398:Homeodomain Proteins; D026161:MADS Domain Proteins; D009687:Nuclear Proteins; D063146:Polycomb-Group Proteins; D016372:RNA, Antisense; D018749:RNA, Plant; D022661:RNA, Untranslated; D011994:Recombinant Proteins; D012097:Repressor Proteins; C513799:SWINGER protein, Arabidopsis; D014157:Transcription Factors; C480553:VIN3 protein, Arabidopsis; C442836:VRN2 protein, Arabidopsis; D012319:RNA Polymerase II