Aucubin modulates Bcl-2 family proteins expression and inhibits caspases cascade in H₂O₂-induced PC12 cells.
In this study, the effect of aucubin on H(2)O(2)-induced apoptosis was studied by using a rat pheochromocytoma (PC12) cell line. We have analyzed the apoptosis of H(2)O(2)-induced PC12 cells, H(2)O(2)-induced apoptosis appeared to correlate with lower Bcl-2 expression, higher Bax expression and sequential activation of caspase-3 leading to cleavage of poly-ADP-ribose polymerase (PARP). Aucubin not only inhibited lower Bcl-2 expression, high Bax expression, but also modulated caspase-3 activation, PARP cleavage, and eventually protected against H(2)O(2)-induced apoptosis. These results indicated that aucubin can obstruct H(2)O(2)-induced apoptosis by regulating of the expression of Bcl-2 and Bax, as well as suppression of caspases cascade activation.
Department of Chemistry and Life Science, Suzhou University, No. 71 Bianhe Road, Suzhou, 234000 Anhui, People's Republic of China.
Hong Yu
HY
Xue
Dong Yan
DY
Niu
Gui Zhen
GZ
Gao
Qiu Ye
QY
Lin
Li Ji
LJ
Jin
Yong Ping
YP
Xu
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D020169:Caspases / Q000378:metabolism*; D002470:Cell Survival / Q000187:drug effects; D002843:Chromatin / Q000648:ultrastructure; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D057888:Iridoid Glucosides / Q000737:chemistry / Q000494:pharmacology*; D015394:Molecular Structure; D016877:Oxidants / Q000494:pharmacology; D016716:PC12 Cells / Q000187:drug effects* / Q000502:physiology / Q000648:ultrastructure; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D015398:Signal Transduction / Q000187:drug effects*; D051028:bcl-2-Associated X Protein / Q000378:metabolism
D002843:Chromatin; D057888:Iridoid Glucosides; D016877:Oxidants; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; C006650:aucubin; D006861:Hydrogen Peroxide; D011065:Poly(ADP-ribose) Polymerases; D020169:Caspases