A novel clean-up method for urine analysis of low-molecular mass aldehydes by capillary electrophoresis with laser-induced fluorescence detection.
A rapid clean-up method using ultra-filtration was developed to remove sample matrix in the determination of low-molecular mass aldehydes in human urine. The ensuing filtrate was derivatized with fluorescein 5-thiosemicarbazide and the labelled aldehydes determined by capillary zone electrophoresis with laser-induced fluorescence detection. Practical aspects related to the effect of the urine sample matrix on the label chemistry and the electrophoretic separation showed that the urine samples must be diluted 20-fold after their ultra-filtration. By using synthetic urine, linear ranges were established in the range of 15-5000 μg/l with limits of detection between 4.5 and 9 μg/l. The intra- and inter-assay precision (relative standard deviation, %) of the aldehydes ranged from 4.1% to 8.4% and 6.1%-9.6%, respectively, and the average specific uncertainty was 149±12 ng. The average recoveries performed on two levels by enriching synthetic urine samples ranged between 94% and 100%. Finally, the proposed method was applied to check low-molecular mass aldehydes in the human urine of a female volunteer to obtain information about the risk in her exposure to these chemicals in the workplace.
Department of Analytical Chemistry, Marie-Curie Building (Annex), Campus of Rabanales University of Cordoba, E-14071 Cordoba, Spain.
Clara Eugenia
CE
Baños
Manuel
M
Silva
D000447:Aldehydes / Q000652:urine*; D019075:Electrophoresis, Capillary / Q000379:methods*; D005260:Female; D005452:Fluoresceins / Q000737:chemistry; D006801:Humans; D007834:Lasers; D012680:Sensitivity and Specificity; D014462:Ultrafiltration
D000447:Aldehydes; D005452:Fluoresceins; C027912:fluorescein thiosemicarbazide