Proteomic changes in maize roots after short-term adjustment to saline growth conditions.
It is of fundamental importance to understand adaptation processes leading to salt resistance. The initial effects on maize roots in the first hour after the adjustment to saline conditions were monitored to elucidate initial responses. The subsequent proteome change was monitored using a 2-D proteomic approach. We found several new salt-inducible proteins, whose expression has not been previously reported to be modulated by salt. A set of phosphoproteins in maize was detected but only ten proteins were phosphorylated and six proteins were dephosphorylated after the application of 25 mM NaCl for 1 h. Some of the phosphorylated maize proteins such as fructokinase, UDP-glucosyl transferase BX9, and 2-Cys-peroxyredoxine were enhanced, whereas an isocitrate-dehydrogenase, calmodulin, maturase, and a 40-S-ribosomal protein were dephosphorylated after adjustment to saline conditions. The initial reaction of the proteome and phosphoproteome of maize after adjustment to saline conditions reveals members of sugar signalling and cell signalling pathways such as calmodulin, and gave hint to a transduction chain which is involved in NaCl-induced signalling. An alteration of 14-3-3 proteins as detected may change plasma membrane ATPase activity and cell wall growth regulators such as xyloglucane endotransglycosylase were also found to be changed immediately after the adjustment to salt stress.
Institute of Plant Nutrition and Soil Science, Christian Albrechts University Kiel, Kiel, Germany. czoerb@plantnutrition.uni-kiel.de
Christian
C
Zörb
Sigrid
S
Schmitt
Karl H
KH
Mühling
D010749:Phosphoprotein Phosphatases / Q000378:metabolism; D010766:Phosphorylation; D010940:Plant Proteins / Q000378:metabolism*; D018517:Plant Roots / Q000378:metabolism*; D011494:Protein Kinases / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D020543:Proteome / Q000378:metabolism*; D055049:Salt Tolerance; D015398:Signal Transduction; D013312:Stress, Physiological; D003313:Zea mays / Q000378:metabolism*
D010940:Plant Proteins; D020543:Proteome; D011494:Protein Kinases; D010749:Phosphoprotein Phosphatases