Caveolin-1 mediates tumor cell migration and invasion and its regulation by miR-133a in head and neck squamous cell carcinoma.
MicroRNAs (miRNAs) are small non-coding RNAs of approximately 22 nucleotides that can function as oncogenes or tumor suppressors in human cancer. Down-regulation of the miRNA miR-133a in many type of cancers, and a reduction of cell proliferation, migration, and invasion upon over-expression, suggests that miR-133a is a tumor suppressor. In this study, genome-wide gene expression analysis of HNSCC cells that over-express miR-133a showed that caveolin-1 (CAV1), a multifunctional scaffolding protein, is down-regulated, a result that was confirmed by real-time PCR and Western blot analysis. A luciferase reporter assay revealed that miR-133a is directly bound to CAV1 mRNA. Cancer cell migration and invasion were significantly inhibited in HNSCC cells transfected with si-CAV1. Therefore, CAV1 functions as an oncogene in HNSCC. The identification of tumor suppressive miRNAs and their target genes could provide new insights into potential mechanism of HNSCC carcinogenesis.
Department of Functional Genomics, Graduate School of Medicine, Chiba University, Chiba, Japan.
Nijiro
N
Nohata
Toyoyuki
T
Hanazawa
Naoko
N
Kikkawa
Muradil
M
Mutallip
Lisa
L
Fujimura
Hirofumi
H
Yoshino
Kazumori
K
Kawakami
Takeshi
T
Chiyomaru
Hideki
H
Enokida
Masayuki
M
Nakagawa
Yoshitaka
Y
Okamoto
Naohiko
N
Seki
D000328:Adult; D000368:Aged; D002277:Carcinoma / Q000235:genetics / Q000473:pathology; D002294:Carcinoma, Squamous Cell; D051242:Caveolin 1 / Q000235:genetics*; D048708:Cell Growth Processes / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006258:Head and Neck Neoplasms / Q000235:genetics / Q000473:pathology; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D018307:Neoplasms, Squamous Cell / Q000235:genetics / Q000473:pathology; D000077195:Squamous Cell Carcinoma of Head and Neck; D014162:Transfection
D051242:Caveolin 1; C548234:MIRN133 microRNA, human; D035683:MicroRNAs