Central administration of neuropeptide FF and related peptides attenuate systemic morphine analgesia in mice.
Neuropeptide FF (NPFF) belongs to an opioid-modulating peptide family. NPFF has been reported to play important roles in the control of pain and analgesia through interactions with the opioid system. However, very few studies examined the effect of supraspinal NPFF system on analgesia induced by opiates administered at the peripheral level. In the present study, intracerebroventricular (i.c.v.) injection of NPFF (1, 3 and 10 nmol) dose-dependently inhibited systemic morphine (0.12 mg, i.p.) analgesia in the mouse tail flick test. Similarly, i.c.v. administration of dNPA and NPVF, two agonists highly selective for NPFF(2) and NPFF(1) receptors, respectively, decreased analgesia induced by i.p. morphine in mice. Furthermore, these anti-opioid activities of NPFF and related peptides were blocked by pretreatment with the NPFF receptors selective antagonist RF9 (10 nmol, i.c.v.). These results demonstrate that activation of central NPFF(1) and NPFF(2) receptors has the similar anti-opioid actions on the antinociceptive effect of systemic morphine.
Key Laboratory of Preclinical Study for New Drugs of Gansu Province, School of Medicine, Lanzhou University, 222 Tian Shui South Road, Lanzhou, 730000, PR China.
Quan
Q
Fang
Tian-nan
TN
Jiang
Ning
N
Li
Zheng-lan
ZL
Han
Rui
R
Wang
D000698:Analgesia; D000701:Analgesics, Opioid / Q000037:antagonists & inhibitors*; D000818:Animals; D004305:Dose-Response Relationship, Drug; D004336:Drug Antagonism; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009020:Morphine / Q000008:administration & dosage / Q000037:antagonists & inhibitors*; D009292:Narcotic Antagonists / Q000008:administration & dosage*; D009479:Neuropeptides / Q000008:administration & dosage; D009842:Oligopeptides / Q000008:administration & dosage*; D010146:Pain / Q000188:drug therapy; D018013:Receptors, Neuropeptide / Q000819:agonists / Q000037:antagonists & inhibitors
D000701:Analgesics, Opioid; D009292:Narcotic Antagonists; D009479:Neuropeptides; D009842:Oligopeptides; D018013:Receptors, Neuropeptide; C083929:neuropeptide FF receptor; C503959:neuropeptide VF; D009020:Morphine; C053651:phenylalanyl-leucyl-phenylalanyl-glutaminyl-prolyl-glutaminyl-arginyl-phenylalaninamide