[Simultaneous determination of tectorigenin-7-O-xylosylglucosid, tectoridin and tectorigenin in flowers of Pueraria lobata by HPLC].
OBJECTIVE
To develop a HPLC method for the simultaneous determination of tectorigenin-7-O-xylosylglucosid, tectoridin and tectorigenin in flowers of Pueraria lobata.


METHODS
The separation was performed on a Kromasil C18 column (4.6 mm x 250 mm, 5 microm), using a gradient elution with acetonitrile-water containing 0.2% phosphoric acid as the mobile phase. The flow rate was 1.0 mL x min(-1), the detection wavelength was 265 nm and the temperature of column was 35 degrees C.


RESULTS
The linear ranges of tectorigenin-7-O-xylosylglucosid, tectoridin and tectorigenin were 0.14-1.40 (r = 0.9994), 0.20-2.02 (r = 0.9993), and 0. 10-1.04 (r = 0.9991) , respectively. The average recoveries (n=6) of the three constituents were 99.5% (RSD 1.8%), 99.6% (RSD 1.7%), and 99.8% (RSD 1.9%), respectively.


CONCLUSIONS
The method is simple and accurate with a good eata and can be used as a quality control method for flowers of P. lobata of different sources.
School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, China. andyliulihui@163.com
Lihui
L
Liu
Weimin
W
Li
Ying
Y
Gao
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D035264:Flowers / Q000737:chemistry; D005960:Glucosides / Q000032:analysis*; D007529:Isoflavones / Q000032:analysis*; D029908:Pueraria / Q000737:chemistry*
D004365:Drugs, Chinese Herbal; D005960:Glucosides; D007529:Isoflavones; C120039:tectorigenin; C120040:tectoridin