[Inhibitory effect of transforming growth factor-β(1) on oral squamous cell carcinoma brain metastasis Tb cell line].
OBJECTIVE
To investigate the inhibitory effect of transforming growth factor (TGF)-β₁ on oral squamous cell carcinoma (OSCC) Tb cell line.


METHODS
Cell counting method was used to examine the inhibitory effect of TGF-β₁ on Tb cell and flow cytometry (FCM) assay performed to measure the changes of cell cycle. Superarray was used to screen the changing expression of genes in TGF-β₁/Smads signaling pathway.RT-PCR method was used to detect the results of Superarray.


RESULTS
TGF-β₁ showed significant inhibiting effect on OSCC Tb cell line. TGF-β₁ blocked the cell cycle at G₁ phase. The expression level of activin receptor-like kinase-1 (ACVRL-1), anti-mullerian hirmine (AMH), cyclim-dependent kinase inhibitor-2B (CDKN-2B) and transforming growth factor-beta-indnced factor (TGIF) was higer in the cells treated with TGF-β₁ than in control, while TDGF-1 expression was down-regulated. ACVRL-1 and CDKN-2B gene expression was consistent with the results of Superarray.


CONCLUSIONS
TGF-β₁ can inhibit the growth of OSCC Tb cell line. The mechanism may be related to the regulation of cell cycle and the expression of ACVRL-1 and CDKN-2B in TGF-β₁-Smads signaling pathway.
Department of Stomatology, The Second Affiliated Hospital of Harbin Medical University, China.
Cheng-min
CM
Liu
Cheng-ren
CR
Zhang
Xiu-mei
XM
Wang
Xu-guang
XG
Xu
Song-bin
SB
Fu
D030301:Activin Receptors, Type II / Q000378:metabolism; D054304:Anti-Mullerian Hormone / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D059447:Cell Cycle Checkpoints; D045744:Cell Line, Tumor; D050762:Cyclin-Dependent Kinase Inhibitor p15 / Q000378:metabolism; D006801:Humans; D009362:Neoplasm Metastasis; D015398:Signal Transduction; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology*
C493966:CDKN2B protein, human; D050762:Cyclin-Dependent Kinase Inhibitor p15; D053773:Transforming Growth Factor beta1; D054304:Anti-Mullerian Hormone; C425364:ACVRL1 protein, human; D030301:Activin Receptors, Type II