A histidine-kinase cheA gene of Pseudomonas pseudoalcaligens KF707 not only has a key role in chemotaxis but also affects biofilm formation and cell metabolism.
A histidine-kinase cheA gene in Pseudomonas pseudoalcaligenes KF707 plays a central role in the regulation of metabolic responses as well as in chemotaxis. Non-chemotactic mutants harboring insertions into the cheA gene were screened for their ability to form biofilms in the Calgary biofilm device. Notably, ≥95% decrease in the number of cells attached to the polystyrene surface was observed in cheA mutants compared to the KF707 wild-type biofilm phenotype. The ability to form mature biofilms was restored to wild-type levels, providing functional copies of the KF707 cheA gene to the mutants. In addition, phenotype micro-arrays and proteomic analyses revealed that several basic metabolic activities and a few periplasmic binding proteins of cheA mutant cells differed compared to those of wild-type cells. These results are interpreted as evidence of a strong integration between chemotactic and metabolic pathways in the process of biofilm development by P. pseudoalcaligenes KF707.
Department of Biological Sciences & Biofilm Research Group, University of Calgary, Calgary, Alberta, Canada.
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Tremaroli
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Fedi
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Tamburini
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Viti
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Tatti
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Ceri
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Turner
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Zannoni
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D018441:Biofilms; D002633:Chemotaxis; D015180:Electrophoresis, Gel, Two-Dimensional; D000071677:Histidine Kinase; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D000072236:Methyl-Accepting Chemotaxis Proteins; D018613:Microscopy, Confocal; D008969:Molecular Sequence Data; D009154:Mutation; D010802:Phylogeny; D011494:Protein Kinases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D044205:Pseudomonas pseudoalcaligenes / Q000145:classification / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry
D001426:Bacterial Proteins; D008565:Membrane Proteins; D000072236:Methyl-Accepting Chemotaxis Proteins; D011494:Protein Kinases; D000071677:Histidine Kinase