The β-adrenoceptor agonist isoproterenol promotes the activity of respiratory chain complex I and lowers cellular reactive oxygen species in fibroblasts and heart myoblasts.
A study is presented on the effect of the β-adrenoceptor agonist isoproterenol on mitochondrial oxygen metabolism in fibroblast and heart myoblast cultures. Isoproterenol treatment of serum-limited fibroblasts and proliferating myoblasts results in the promotion of mitochondrial complex I activity and decrease of the cellular level of reactive oxygen species. These effects of isoproterenol are associated with cAMP-dependent phosphorylation of complex I subunit(s). Addition of okadaic acid, inhibitor of protein phosphatase(s), reverses the decline of complex I activity in serum-limited fibroblast cultures and activates the complex in proliferating myoblast cultures. The effects of isoproterenol on complex I activity and reactive oxygen species balance can contribute to the therapeutic effect of the drug.
Department of Medical Biochemistry, Biology and Physics (DIBIFIM), University of Bari Aldo Moro, 70124, Bari, Italy. a.signorile@biochem.uniba.it
Domenico
D
De Rasmo
Giuliano
G
Gattoni
Francesco
F
Papa
Arcangela
A
Santeramo
Consiglia
C
Pacelli
Tiziana
T
Cocco
Loris
L
Micelli
Nicola
N
Sardaro
Maria
M
Larizza
Michele
M
Scivetti
Serena
S
Milano
Anna
A
Signorile
D000318:Adrenergic beta-Agonists / Q000494:pharmacology*; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism; D042967:Electron Transport Complex I / Q000378:metabolism*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D007231:Infant, Newborn; D007545:Isoproterenol / Q000494:pharmacology*; D008928:Mitochondria / Q000378:metabolism; D032386:Myoblasts, Cardiac / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D010766:Phosphorylation; D017382:Reactive Oxygen Species / Q000378:metabolism*
D000318:Adrenergic beta-Agonists; D017382:Reactive Oxygen Species; D000242:Cyclic AMP; D042967:Electron Transport Complex I; D007545:Isoproterenol