Efficient conjugation doxorubicin to bacterial magnetic nanoparticles via a triplex hands coupling reagent.
By using a novel triplex "hand" coupling reagent, tris-succinimidyl aminotriacetate (TSAT), doxorubicin loading efficiency of BMP can be greatly enhanced in comparison with that of dual functional linkers. The drug loading ratio of doxorubicin-coupled bacterial magnetic nanoparticles produced by this method (DBMP-T) is 62%. DBMP-T disperse well, have a narrow size distribution, exhibit a high magnetic response, is sensitive to pH to facilitate drug release and are strongly cytotoxic to HePG2 and MCF-7 cancer cells.
Key Laboratory of Mesoscopic Chemistry of MOE and School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, PR China.
Lin
L
Guo
Ji
J
Huang
Li-Min
LM
Zheng
D006528:Carcinoma, Hepatocellular / Q000188:drug therapy / Q000378:metabolism; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004317:Doxorubicin / Q000008:administration & dosage / Q000737:chemistry* / Q000493:pharmacokinetics; D005976:Glutaral / Q000737:chemistry; D056945:Hep G2 Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008027:Light; D008113:Liver Neoplasms / Q000188:drug therapy / Q000378:metabolism; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D042201:Magnetospirillum / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D010316:Particle Size; D012542:Scattering, Radiation; D013055:Spectrophotometry, Infrared; D013388:Succinimides / Q000737:chemistry*
D058185:Magnetite Nanoparticles; D013388:Succinimides; C555440:tris-succinimidyl aminotriacetate; D004317:Doxorubicin; D005976:Glutaral