The 5' and 3' downstream AUG region elements are required for mosquito-borne flavivirus RNA replication.
Flaviviruses require complementarity between the 5' and 3' ends of the genome for RNA replication. For mosquito-borne flaviviruses, the cyclization sequences (CS) and upstream of AUG region (UAR) elements at the genomic termini are necessary for viral RNA replication, and a third motif, the downstream of AUG region (DAR), was recently designated for dengue virus. The 3' DAR sequence is also part of a hairpin (HP-3'SL), and both complementarity between 5' and 3' DAR motifs and formation of the HP-3'SL in the absence of the 5' end are conserved among mosquito-borne flaviviruses. Using West Nile virus as a model, we demonstrate that 5'-3' DAR complementarity and HP-3'SL formation are essential for viral RNA replication.
Division of Infectious Diseases and Vaccinology, School of Public Health, University of California, Berkeley, Berkeley, California 94720-7354, USA.
Peter
P
Friebe
Pei-Yong
PY
Shi
Eva
E
Harris
D020413:3' Untranslated Regions / Q000235:genetics* / Q000502:physiology; D020121:5' Untranslated Regions / Q000235:genetics* / Q000502:physiology; D000818:Animals; D001483:Base Sequence; D009033:Culicidae / Q000821:virology*; D005416:Flavivirus / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012093:Replicon; D014779:Virus Replication; D014902:West Nile virus / Q000235:genetics / Q000378:metabolism
D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D012367:RNA, Viral