Possible involvement of loss of heterozygosity in malignant transformation of ovarian endometriosis.
OBJECTIVE
Although histological and epidemiological studies have suggested that endometriosis has malignant potential, the molecular mechanism underlying the malignant transformation of endometriosis is poorly understood. Ovarian cancer arising from endometriosis (OCEM) may provide an ideal model for genetic studies. To investigate the genetic alterations during transformation of ovarian endometriosis into cancer, we analysed loss of heterozygosity (LOH) and mutations of tumour-related genes in OCEM cases (n=12) that fulfilled the histological criteria and in solitary ovarian endometriosis (n=12).


METHODS
Each paraffin-embedded section was microdissected to isolate the endometriotic epithelium, transitional epithelium, and cancer cells. Extracted DNA was amplified by nested PCR. LOH was identified with fluorescence-labelled microsatellite markers. Mutations of tumour-related genes PTEN, TP53, and K-ras were examined by bidirectional DNA sequencing.


RESULTS
With 13 microsatellite markers on six chromosomes, we detected 31 and eight LOH events in OCEMs and in solitary ovarian endometriosis, respectively. In the OCEM group, 18 LOH events were found in cancer cells alone, while 13 LOH events were found in all cancer, transitional, and endometriotic tissues. High frequencies of LOH were detected on chromosomes 9p, 10q, and 13q. However, only two point mutations were detected in cancer cells in one case.


CONCLUSIONS
Our study suggested that accumulated LOH events on some chromosome loci may be involved in malignant transformation of endometriosis, while mutations in certain tumour-related genes are not.
Department of Obstetrics and Gynecology, Kyoto Prefectural University of Medicine, Graduate School of Medical Science, 465 Kajii-cho, Kamigyo-ku, Kyoto 602-8566, Japan.
Bing
B
Xu
Shinshichi
S
Hamada
Izumi
I
Kusuki
Ryoji
R
Itoh
Jo
J
Kitawaki
D018262:Adenocarcinoma, Clear Cell / Q000235:genetics / Q000473:pathology; D000328:Adult; D018269:Carcinoma, Endometrioid / Q000235:genetics / Q000473:pathology; D002471:Cell Transformation, Neoplastic / Q000235:genetics* / Q000473:pathology; D004715:Endometriosis / Q000235:genetics* / Q000473:pathology; D005260:Female; D016147:Genes, Tumor Suppressor; D016158:Genes, p53; D011905:Genes, ras; D006801:Humans; D019656:Loss of Heterozygosity; D018895:Microsatellite Repeats; D008875:Middle Aged; D009154:Mutation; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000473:pathology; D051059:PTEN Phosphohydrolase / Q000235:genetics; D016612:Paraffin Embedding; D055815:Young Adult
D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human