Frequent MSI mononucleotide markers for diagnosis of hereditary nonpolyposis colorectal cancer.
BACKGROUND
Failure in the DNA mismatch repair system is commonly accompanied by microsatellite instability and leads to colorectal cancer. The aim of this study was to find the most frequent of five mononucleotide markers in order to devise the simplest diagnostic strategy for identification of patients with hereditary nonpolyposis colorectal cancer (HNPCC) who were defined by defects in mismatch repair system.


METHODS
78 patients with colorectal cancer were recruited for this investigation. Five mononucleotide markers, NR-27, NR-21, NR-24, BAT-25 and BAT-26, were used as a pentaplex panel to determine MSI status.


RESULTS
Two out of five mononucleotide markers, NR-21 (25.6%) and BAT-25 (23.1%) showed more instability than the others.


CONCLUSIONS
In defining individuals with colorectal cancer, BAT25 and NR-21 may provide diagnostic assistance.
Science and Research Branch, Islamic Azad University (IAU), and Research Center for Gastroenterology and Liver Diseases, Taleghani Hospital, Tehran, Iran. mah_haghighi@hotmail.com
Mahdi Montazer
MM
Haghighi
Gholam Reza
GR
Javadi
Kazem
K
Parivar
Saman
S
Milanizadeh
Narges
N
Zali
Seyed Reza
SR
Fatemi
Mohammad Reza
MR
Zali
D015415:Biomarkers; D015179:Colorectal Neoplasms / Q000175:diagnosis / Q000235:genetics*; D003123:Colorectal Neoplasms, Hereditary Nonpolyposis / Q000175:diagnosis / Q000235:genetics*; D053843:DNA Mismatch Repair; D005819:Genetic Markers; D006801:Humans; D053842:Microsatellite Instability; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction
D015415:Biomarkers; D005819:Genetic Markers