Effects of light intensity and dilution rate on the semicontinuous cultivation of Arthrospira (Spirulina) platensis. A kinetic Monod-type approach.
Semicontinuous cultures were carried out at different dilution rates (D) and light intensities (I) to determine the maximum productivity of Arthrospira platensis cultivated in helicoidal photobioreactor up to the achievement of pseudo-steady-state conditions. At I=108 μmol photons m(-2) s(-1), the semicontinuous regime ensured the highest values of maximum cell concentration (X(m)=5772±113 mg L(-1)) and productivity (P(XS)=1319±25 mg L(-1) d(-1)) at the lowest (D=0.1 day(-1)) and the highest (D=0.3 day(-1)) dilution rates, respectively. A kinetic model derived from that of Monod was proposed to determine the relationship between the product of light intensity to dilution rate (ID) and the cell productivity, which were shown to exert a combined influence on this parameter. This result put into evidence that pseudo-steady-state conditions could be modified according to circumstances, conveniently varying one or other of the two independent variables.
Department of Biochemical and Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, University of São Paulo, São Paulo-SP, Brazil.
Raquel Pedrosa
RP
Bezerra
Erika Yuliana Ortiz
EY
Montoya
Sunao
S
Sato
Patrizia
P
Perego
João Carlos Monteiro
JC
de Carvalho
Attilio
A
Converti
D049109:Cell Proliferation / Q000528:radiation effects; D003198:Computer Simulation; D008027:Light; D008954:Models, Biological; D058433:Photobioreactors / Q000382:microbiology*; D011829:Radiation Dosage; D053545:Spirulina / Q000502:physiology* / Q000528:radiation effects*