Bioassay-guided separation of an alpha-amylase inhibitor anthocyanin from Vaccinium arctostaphylos berries.
Vaccinium arctostaphylos is a traditional medicinal plant in Iran used for the treatment of diabetes mellitus. In our search for antidiabetic compounds from natural sources, we found that the extract obtained from V. arctostaphylos berries showed an inhibitory effect on pancreatic alpha-amylase in vitro [IC50 = 1.91 (1.89-1.94) mg/mL]. The activity-guided purification of the extract led to the isolation of malvidin-3-O-beta-glucoside as an a-amylase inhibitor. The compound demonstrated a dose-dependent enzyme inihibitory activity [IC50 = 0.329 (0.316-0.342) mM].
Department of Pharmacognosy, School of Pharmacy, Shahid Beheshti University of Medical Sciences, P.O. Box 14155-6153, Tehran, Iran. bnickavar@sbmu.ac.ir
Bahman
B
Nickavar
Gholamreza
G
Amin
D000872:Anthocyanins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000302:isolation & purification / Q000494:pharmacology; D005638:Fruit / Q000737:chemistry; D007492:Iran; D008958:Models, Molecular; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D029796:Vaccinium / Q000737:chemistry*; D000516:alpha-Amylases / Q000037:antagonists & inhibitors*
D000872:Anthocyanins; D004791:Enzyme Inhibitors; D010936:Plant Extracts; D000516:alpha-Amylases