Oligonucleotide-stabilized fluorescent silver nanoclusters for sensitive detection of biothiols in biological fluids.
In this work, we reported a simple and sensitive method to detect biothiols, such as cysteine (Cys), homocysteine (Hcy) and glutathione (GSH), using fluorescent silver nanoclusters (Ag NCs) stabilized by single-stranded DNA (DNA-Ag NCs) as probes. The photoluminescence intensity of DNA-Ag NCs was found to be quenched effectively with the increase of biothiols concentration due to the formed nonfluorescent coordination complex between DNA-Ag NCs and biothiols, resulting in the shift-to-red of emission wavelength. But the fluorescence of DNA-Ag NCs was not changed in the presence of other amino acids at 10-fold higher concentration. Satisfactory detection limits and linear relationships of Cys, GSH and Hcy were obtained, respectively. The resulted plots exhibited good linear relationships in the range from 8.0×10(-9) to 1.0×10(-7) mol L(-1) (R(2)=0.984) for Cys, 8.0×10(-9) to 1.0×10(-7) mol L(-1) (R(2)=0.983) for GSH, and 2.0×10(-6) to 6.0×10(-7) mol L(-1) (R(2)=0.999) for Hcy, respectively; the detection limits of Cys, GSH and Hcy were 4.0 nmol L(-1), 4.0 nmol L(-1), and 0.2 μmol L(-1), respectively. The method was successfully used for the detection of biothiols in human plasma samples.
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Changchun, Jilin 130022, China.
Bingyan
B
Han
Erkang
E
Wang
D015374:Biosensing Techniques / Q000295:instrumentation*; D004247:DNA / Q000737:chemistry*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D012834:Silver / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000295:instrumentation*; D013438:Sulfhydryl Compounds / Q000097:blood*
D013438:Sulfhydryl Compounds; D012834:Silver; D004247:DNA