Immunomodulatory effect of Moringa oleifera Lam. extract on cyclophosphamide induced toxicity in mice.
Immunomodulatory effect of ethanolic extract (50%) of M. oleifera leaves (MOE) has been studied in normal and immunosuppressed mice models. Different doses of MOE i.e. 125, 250 and 500 mg/kg body weight of mice were administered orally for 15 days. Cyclophosphamide at a dose of 30 mg/kg body weight was administered orally for the next 3 days. On day 16 and 19, hematological parameters like white blood cell (WBC) count, red blood cell (RBC) count, haemoglobin level (Hb), percent neutrophils and organ weight were recorded. Effect of MOE on phagocytic activity of mice macrophages was determined by carbon clearance test. MOE showed significant dose dependent increase in WBC, percent neutrophils, weight of thymus and spleen along with phagocytic index in normal and immunosuppressed mice. The results indicate that MOE significantly reduced cyclophosphamide induced immunosuppression by stimulating both cellular and humoral immunity.
Pharmacognosy and Ethnopharmacology Division, National Botanical Research Institute, Rana Pratap Marg, Lucknow 226 001, India.
Anamika
A
Gupta
Manish K
MK
Gautam
Rahul K
RK
Singh
M Vijay
MV
Kumar
Ch V
ChV
Rao
R K
RK
Goel
Shampa
S
Anupurba
D000284:Administration, Oral; D000818:Animals; D003520:Cyclophosphamide / Q000008:administration & dosage / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D016867:Immunocompromised Host; D007155:Immunologic Factors / Q000008:administration & dosage / Q000494:pharmacology*; D007166:Immunosuppressive Agents / Q000008:administration & dosage / Q000633:toxicity; D007958:Leukocyte Count; D008297:Male; D051379:Mice; D029588:Moringa oleifera / Q000737:chemistry*; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects; D009929:Organ Size / Q000187:drug effects; D010587:Phagocytosis / Q000187:drug effects / Q000276:immunology; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D013154:Spleen / Q000187:drug effects / Q000254:growth & development; D013950:Thymus Gland / Q000187:drug effects / Q000254:growth & development
D007155:Immunologic Factors; D007166:Immunosuppressive Agents; D010936:Plant Extracts; D003520:Cyclophosphamide