PAIR3, an axis-associated protein, is essential for the recruitment of recombination elements onto meiotic chromosomes in rice.
During meiosis, the paired homologous chromosomes are tightly held together by the synaptonemal complex (SC). This complex consists of two parallel axial/lateral elements (AEs/LEs) and one central element. Here, we observed that PAIR3 localized to the chromosome core during prophase I and associated with both unsynapsed AEs and synapsed LEs. Analyses of the severe pair3 mutant demonstrated that PAIR3 was essential for bouquet formation, homologous pairing and normal recombination, and SC assembly. In addition, we showed that although PAIR3 was not required for the initial recruitment of PAIR2, it was required for the proper association of PAIR2 with chromosomes. Dual immunostaining revealed that PAIR3 highly colocalized with REC8. Moreover, studies using a rec8 mutant indicated that PAIR3 localized to chromosomes in a REC8-dependent manner.
State Key Laboratory of Plant Genomics and Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
Kejian
K
Wang
Mo
M
Wang
Ding
D
Tang
Yi
Y
Shen
Baoxiang
B
Qin
Ming
M
Li
Zhukuan
Z
Cheng
D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism; D023902:Chromosome Pairing; D032461:Chromosomes, Plant / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D048749:Cytokinesis; D017404:In Situ Hybridization, Fluorescence; D008540:Meiosis; D049469:Meiotic Prophase I / Q000235:genetics; D009154:Mutation; D009630:Nondisjunction, Genetic; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D012275:Oryza / Q000235:genetics* / Q000502:physiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D011058:Pollen; D011995:Recombination, Genetic; D013573:Synaptonemal Complex / Q000378:metabolism* / Q000648:ultrastructure; D016615:Telomere / Q000648:ultrastructure
D018797:Cell Cycle Proteins; D009687:Nuclear Proteins; D010940:Plant Proteins