Isolation and structure determination of a cholesterol esterase inhibitor from Ganoderma lucidum.
Bioassay-guided fractionation of methanol extract of Ganoderma lucidum gave a pure cholesterol esterase inhibitor. On the basis of spectroscopic analysis and comparison with data from the literature, its structure was identified as 5alpha,8alpha-epidioxyergosta-6,22-dien-3beta-ol (compound I). Compound (I) inhibited cholesterol esterase with an IC50 value of 42 micronM. A Lineweaver-Burk plot revealed that the compound (I) is a noncompetitive inhibitor. Findings from this study suggest that compound (I) may be the active principle of the hypocholesterolemic effect of Ganoderma lucidum.
Department of Biological Engineering, Seokyung University, Seoul 136-704, Korea. sdkim@skuniv.ac.kr
Shin Duk
SD
Kim
D000818:Animals; D004791:Enzyme Inhibitors / Q000737:chemistry* / Q000302:isolation & purification*; D004875:Ergosterol / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification; D007700:Kinetics; D015394:Molecular Structure; D020940:Reishi / Q000737:chemistry*; D002787:Sterol Esterase / Q000037:antagonists & inhibitors* / Q000737:chemistry; D013552:Swine
C503471:5alpha,8alpha-epidioxyergosta-6,22-dien-3-beta-ol; D004791:Enzyme Inhibitors; D002787:Sterol Esterase; D004875:Ergosterol