Effect of blood sample type on the measurement of advanced oxidation protein products as a biomarker of inflammation and oxidative stress in hemodialysis patients.
Advanced oxidation protein products (AOPP) is widely used as a uremic biomarker, especially for cardiovascular disease. However, it has not been determined whether it is better to measure AOPP in plasma or serum. In this cross-sectional study, which included 102 patients undergoing maintenance hemodialysis, fibrinogen-free serum and defibrinated plasma samples were prepared. AOPP levels from fibrinogen-free samples displayed a stronger correlation with myeloperoxidase activity and levels of C-reactive protein, interleukin-6 and tumor necrosis factor-alpha, as well as prevalent cardiovascular disease, than AOPP levels obtained from plasma samples. These results demonstrated that fibrinogen interferes with the measurement of AOPP.
Division of Nephrology, Guangdong General Hospital, Guangdong Academy of Medical Sciences, China.
Yuan-Han
YH
Chen
Wei
W
Shi
Xin-Ling
XL
Liang
Yong-Zheng
YZ
Liang
Xia
X
Fu
D015415:Biomarkers / Q000097:blood; D001800:Blood Specimen Collection / Q000379:methods / Q000592:standards; D002097:C-Reactive Protein / Q000032:analysis; D002318:Cardiovascular Diseases / Q000097:blood; D003430:Cross-Sectional Studies; D005260:Female; D005340:Fibrinogen / Q000009:adverse effects / Q000032:analysis* / Q000737:chemistry; D006801:Humans; D007249:Inflammation / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D008297:Male; D008875:Middle Aged; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D009195:Peroxidase / Q000032:analysis; D006435:Renal Dialysis; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
D015415:Biomarkers; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D005340:Fibrinogen; D002097:C-Reactive Protein; D009195:Peroxidase