Cathepsins (S and B) and their inhibitor Cystatin C in immune cells: modulation by interferon-β and role played in cell migration.
We investigated the modulation of Cathepsin S, Cathepsin B and Cystatin C expression in immune cells by interferon (IFN)-β, and their role in cell migration. Cathepsin levels were increased in monocytic and T line cells upon activation. IFN-β abolished this increase of Cathepsin B in monocytes and of Cathepsin S in T cells, while increased Cystatin C. Neutralizing Cystatin C increased cell migration, while migration of monocytes and T cells was inhibited by IFN-β, by Cystatin C, and T cell migration was suppressed by Cathepsin S and B inhibitors. These findings support further studies in the importance of Cathepsins and Cystatins in immunomodulation.
Rappaport Faculty of Medicine and Research Institute, Technion-Israel Institute of Technology, Haifa, Israel.
Elsebeth
E
Staun-Ram
Ariel
A
Miller
D015153:Blotting, Western; D002401:Cathepsin B / Q000276:immunology / Q000378:metabolism*; D002403:Cathepsins / Q000276:immunology / Q000378:metabolism*; D002465:Cell Movement; D002634:Chemotaxis, Leukocyte / Q000502:physiology*; D055316:Cystatin C / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D016899:Interferon-beta / Q000276:immunology / Q000378:metabolism*; D019169:Jurkat Cells; D009000:Monocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D020298:U937 Cells
C525030:CST3 protein, human; D055316:Cystatin C; D016899:Interferon-beta; D002403:Cathepsins; C533912:CTSB protein, human; D002401:Cathepsin B; C028217:cathepsin S