[Pharmacology experimental study of new hematischesis compounds after Flos Sophorae carbonized].
OBJECTIVE
To search new effective compounds, the different hemostatic effects of Flos Sophorae, Flos Sophorae Carbonisatus and principal constituent were observed.


METHODS
Using the bleeding time (BT) and the recalcification time (RT) as the specificity indicators for the hematischesis function, the hemostatic effects of the following were observed. Flos Sophorae, Flos Sophorae Carbonisatus, characteristic value extraction thing A and B (SCE A and B) and the principal constituent after orally administered in normal rats in order to analyze the new hemostatic compounds.


RESULTS
Flos Sophorae, and Flos Sophorae Carbonisatus can obviously reduce BT and RT in rats, in which the effect of Carbonisatus is stronger than the crude. Otherwhile, SCE A and SCE B can also obviously reduce BT and RT in rats, in which the effects of SCE B surpassed those of SCE A. Furthermore, two characteristic compounds extracted from SCE B (kaikasaponin I called compound 1 and isorhamnetin-3-O-rutinoside named called 2) and other nominated principal constituents (rutin, tannin), can obviously shorten BT and RT in rats, among which compound 2 is most superior.


CONCLUSIONS
The Flos Sophorae, Flos Sophorae Carbonisatus and their character compounds can shorten! the BT and RT in rats. The compound 2 from SCE B has the most superior effect. Study showed that compound 2 should be the new hemostatic compounds after Flos Sophorae carbonized. The results also indicated that the increase of hemostatic effect after Flos Sophorae carbonized should be related with the coordination of various kinds of ingredient.
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China. yonger99@sina.com
Yong
Y
Zhao
Jing
J
Guo
Ting
T
Liu
Chunying
C
Li
Chunyu
C
Cao
Yan
Y
Yi
Ran
R
Hao
Raorao
R
Li
D000818:Animals; D001760:Bleeding Time; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D005260:Female; D035264:Flowers / Q000737:chemistry; D006490:Hemostatics / Q000008:administration & dosage*; D008297:Male; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D029909:Sophora / Q000737:chemistry*
D004365:Drugs, Chinese Herbal; D006490:Hemostatics