Antiproliferative and apoptotic sesquiterpene lactones from Carpesium faberi.
Four new (1-4) and 13 known (5-17) sesquiterpene lactones along with two known diterpenes (18, 19) were isolated from the whole plant of Carpesium faberi. The new structures were elucidated by means of spectroscopic techniques and some chemical transformations to be pseudoguaian-1α(H)-8α,12-olide-4β-O-β-d-glucopyranoside (1), 4β,10α-dihydroxy-5α(H)-1,11(13)-guaidien-8α,12-olide (2), 4β,10β-dihydroxy-5α(H)-1, 11(13)-guaidien-8β,12-olide (3), and (4S)-acetyloxyl-11(13)-carabren-8β,12-olide (4). All isolates were tested against MCF-7 human breast cancer cells using the MTT assay. Among them, the sesquiterpene lactones (except tomentosin 17) possessing an α-methylene-γ-lactone moiety were found to have in vitro antiproliferative activities, with IC(50) values of 3.0-38.8μg/mL. The effects of four selected sesquiterpene lactones (guaianolide 2, carabranolide 4, pseudoguaianolide 9, eudesmanolide 13) on the cell cycle were examined using flow cytometry (FCM).
Department of Natural Products for Chemical Genetic Research, Key Laboratory of Brain Functional Genomics, East China Normal University, No 3663 Zhongshan Road N, Shanghai 200062, PR China.
Xu-Wen
XW
Li
Liang
L
Weng
Xue
X
Gao
Yun
Y
Zhao
Fei
F
Pang
Jian-Hui
JH
Liu
Hong-Feng
HF
Zhang
Jin-Feng
JF
Hu
D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry* / Q000302:isolation & purification / Q000633:toxicity; D017209:Apoptosis; D019659:Asteraceae / Q000737:chemistry*; D045744:Cell Line, Tumor; D006801:Humans; D007783:Lactones / Q000737:chemistry* / Q000302:isolation & purification / Q000633:toxicity; D009682:Magnetic Resonance Spectroscopy; D008968:Molecular Conformation; D010936:Plant Extracts / Q000737:chemistry; D012717:Sesquiterpenes / Q000737:chemistry*
D000972:Antineoplastic Agents, Phytogenic; D007783:Lactones; D010936:Plant Extracts; D012717:Sesquiterpenes