Glycosaminoglycans from marine clam Meretrix meretrix (Linne.) are an anticoagulant.
The extracted unfractionated glycosaminoglycans (GAGs) obtained from the marine clam Meretrix meretrix were fractionated by ion-exchange (Amberlite IRA-900 and 120) chromatography. The fractionated sample activity was determined through azure-A by metachromatic activity and agarose gel electrophoresis. The active fractionated sample molecular mass was determined through gradient polyacrylamide gel electrophoresis (PAGE). The structural characterization of low-molecular-weight GAGs was analyzed by Fourier transform infrared (FT-IR) and ¹H-nuclear magnetic resonance (NMR) spectroscopy. The Activated partial thromboplastin time (APTT) of fractionated heparin-like glycosminoglycan is 72 IU/mg and it has a molecular mass of 15,000 Da. The disaccharide profiles, such as uronic acid 15.31%, hexosamine 24.61%, and sulfate content 11.7%, were also determined. The results of this study suggest that the GAGs from M. meretrix could be an alternative source of heparin.
Centre of Advanced Study in Marine Biology, Annamalai University, Parangipettai, Tamil Nadu, India. saran_prp@yahoo.com
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R
Saravanan
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S
Vairamani
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A
Shanmugam
D000818:Animals; D000925:Anticoagulants / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D049872:Bivalvia / Q000737:chemistry*; D001777:Blood Coagulation / Q000187:drug effects*; D004591:Electrophoresis, Polyacrylamide Gel; D006025:Glycosaminoglycans / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D006801:Humans; D019906:Nuclear Magnetic Resonance, Biomolecular; D010314:Partial Thromboplastin Time; D017550:Spectroscopy, Fourier Transform Infrared
D000925:Anticoagulants; D006025:Glycosaminoglycans