Peroxiredoxin 2 as a chemotherapy responsiveness biomarker candidate in osteosarcoma revealed by proteomics.
OBJECTIVE
We aimed to identify novel chemotherapy responsiveness biomarkers for osteosarcoma (OS) by investigating the global protein expression profile of 12 biopsy samples from OS patients.


METHODS
Six patients were classified as good responders and six as poor responders, according to the Huvos grading system. The protein expression profiles obtained by 2-D DIGE consisted of 2250 protein spots.


RESULTS
Among them, we identified 55 protein spots whose intensity was significantly different (Bonferroni adjusted p-value<0.01) between the two patient groups. Mass spectrometric protein identification demonstrated that the 55 spots corresponded to 38 distinct gene products including peroxiredoxin 2 (PRDX 2). Use of a specific antibody against PRDX 2 confirmed the differential expression of PRDX 2 between good and poor responders, while PRDX 2 levels as measured by Western blotting correlated highly with their corresponding 2-D DIGE values. The predictive value of PRDX 2 expression was further confirmed by examining an additional four OS cases using Western blotting.


CONCLUSIONS
These results establish PRDX 2 as a candidate for chemotherapy responsiveness marker in OS. Measuring PRDX 2 in biopsy samples before treatment may contribute to more effective management of OS.
Proteome Bioinformatics Project, National Cancer Center Research Institute, Tokyo, Japan.
Kazutaka
K
Kikuta
Naobumi
N
Tochigi
Shigeru
S
Saito
Tadakazu
T
Shimoda
Hideo
H
Morioka
Yoshiaki
Y
Toyama
Ako
A
Hosono
Yoshiyuki
Y
Suehara
Yasuo
Y
Beppu
Akira
A
Kawai
Setsuo
S
Hirohashi
Tadashi
T
Kondo
D000293:Adolescent; D000328:Adult; D014408:Biomarkers, Tumor / Q000378:metabolism; D001859:Bone Neoplasms / Q000188:drug therapy* / Q000378:metabolism; D002648:Child; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D012516:Osteosarcoma / Q000188:drug therapy* / Q000378:metabolism; D054464:Peroxiredoxins / Q000378:metabolism*; D011379:Prognosis; D040901:Proteomics / Q000379:methods
D014408:Biomarkers, Tumor; C086065:PRDX2 protein, human; D054464:Peroxiredoxins