Hydroxy and methoxy substituted thiacarbocyanines for fluorescent detection of amyloid formations.
In present paper series of trimethine cyanines modified in 5,5'- or 6,6'- position with hydroxy- or methoxy- substituents is studied for their ability to interact selectively with fibrillar formations. Processes of dye aggregation that accompany this interaction were also investigated. Meso-methyl trimethynecyanines with 5,5'- methoxy (7519) and hydroxy (7515) substituents strongly (up to 40 times) increase fluorescence intensity in the presence of fibrillar insulin, and also give noticeable fluorescent response on the presence of various aggregated proteins (lysozyme, β-lactoglobulin, α-synuclein A53T). 7519 and 7515 dyes can be used for fluorometric detection of fibrillar insulin at concentrations of approximately 1.5-120 microg/ml. For meso-ethyl substituted dye 7514 the ability to form H- and J-aggregates upon interaction with insulin fibrils was suggested. The model of the H- and J-aggregate packing in the protein fibrillar structure has been proposed.
Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, 150 Zabolotnogo St., 03143 Kyiv, Ukraine.
Kateryna D
KD
Volkova
Vladyslava B
VB
Kovalska
Mykhaylo Yu
MY
Losytskyy
Kateryna O
KO
Fal
Nadiya O
NO
Derevyanko
Yuriy L
YL
Slominskii
Olexiy I
OI
Tolmachov
Sergiy M
SM
Yarmoluk
D000682:Amyloid / Q000737:chemistry*; D000818:Animals; D002232:Carbocyanines / Q000737:chemistry*; D002417:Cattle; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D007328:Insulin / Q000737:chemistry; D016014:Linear Models; D055503:Protein Multimerization; D017433:Protein Structure, Secondary; D013050:Spectrometry, Fluorescence
D000682:Amyloid; D002232:Carbocyanines; D005456:Fluorescent Dyes; D007328:Insulin