Role of reactive oxygen species and Bcl-2 family proteins in TNF-α-induced apoptosis of lymphocytes.
We studied the in vitro apoptosis-inducing effect of recombinant TNF-α (rTNF-α) on blood lymphocytes from healthy donors. rTNF-α-induced apoptosis was accompanied by an increase in the number of cells with low mitochondrial transmembrane potential, increased intracellular content of reactive oxygen species, reduced content of Bcl-2, Bcl-xL, and Bax proteins, and elevated Bad content. The molecular mechanisms of these changes are discussed.
Department for Fundamental Bases of Clinical Medicine, Siberian State Medical University, Russian Ministry of Health Care and Social Development, Tomsk, Russia. rnv@mail.ssmu.ru
N V
NV
Ryazanceva
V V
VV
Novickiy
O B
OB
Zhukova
A K
AK
Biktasova
O E
OE
Chechina
E V
EV
Sazonova
M V
MV
Belkina
N Yu
NY
Chasovskih
Z K
ZK
Khaitova
D000328:Adult; D017209:Apoptosis / Q000502:physiology*; D020169:Caspases / Q000378:metabolism; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000166:cytology / Q000502:physiology*; D008297:Male; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*; D018124:Receptors, Tumor Necrosis Factor / Q000378:metabolism; D018709:Statistics, Nonparametric; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D019253:Proto-Oncogene Proteins c-bcl-2; D017382:Reactive Oxygen Species; D018124:Receptors, Tumor Necrosis Factor; D014409:Tumor Necrosis Factor-alpha; D020169:Caspases