Serum paraoxonase activity and oxidative DNA damage in patients with laryngeal squamous cell carcinoma.
OBJECTIVE
To determine the possible role of oxidants and antioxidants in the pathogenesis of in laryngeal squamous cell carcinoma.


METHODS
Our study involved patients with newly diagnosed laryngeal cancer (n = 29) and same age- and sex-matched healthy individuals (n = 21). Serum malondialdehyde (MDA) and paraoxonase (PON1) levels were measured by colorimetric methods and 8-hydroxy-2'-deoxyguanosine (8-OH-dG) was measured using enzyme-linked immunosorbent assay in fasting blood samples of participants.


RESULTS
The levels of 8-OH-dG (control, 4.61 ± 1.27 ng/mL; patient, 11.70 ± 2.44 ng/mL; P < 0.001) and MDA (control, 4.16 ± 1.02 nmol/mL; patient, 8.74 ± 1.65 nmol/mL; P < 0.001) were significantly higher, and those of PON1 (control, 170.86 ± 72.46 U/mL; patient, 80.44 ± 29.81 U/mL; P < 0.001) were significantly lower in patients. There were no statistically significant differences in the 8-OH-dG, MDA levels, and PON1 activity in relation to T (tumor) staging of differentiation and different smoking/drinking status. There was a statistically significant difference in MDA levels (10.24 ± 0.64 nmol/mL) only in stage II laryngeal cancer. There were a statistically significant positive correlation between serum MDA and 8-OH-dG (r = 0.887, P < 0.001), a statistically significant negative correlation between serum MDA and serum PON1 (r = -0.477, P < 0.01), and a statistically significant negative correlation between serum 8-OH-dG and serum PON1 in patients (r = -0.420, P < 0.05).


CONCLUSIONS
We conclude that, in patients with laryngeal squamous cell carcinoma, the oxidant/antioxidant balance was impaired in favor of lipid peroxidation and DNA damage.
Department of Otolaryngology-Head and Neck Surgery, Cerrahpasa Medical Faculty, University of Istanbul, Turkey.
Emin
E
Karaman
Hafize
H
Uzun
Irfan
I
Papila
Huriye
H
Balci
Alper
A
Ozdilek
Habibe
H
Genc
Halil
H
Yanardag
Cigdem
C
Papila
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000368:Aged; D000369:Aged, 80 and over; D000428:Alcohol Drinking; D043303:Aryldialkylphosphatase / Q000097:blood*; D002294:Carcinoma, Squamous Cell / Q000097:blood* / Q000235:genetics; D016022:Case-Control Studies; D008076:Cholesterol, HDL / Q000097:blood; D003124:Colorimetry; D004249:DNA Damage / Q000235:genetics; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D016166:Free Radical Scavengers / Q000097:blood*; D006801:Humans; D007202:Indicators and Reagents; D007822:Laryngeal Neoplasms / Q000097:blood* / Q000235:genetics; D015227:Lipid Peroxidation / Q000502:physiology; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008875:Middle Aged; D009367:Neoplasm Staging; D018384:Oxidative Stress / Q000502:physiology*; D012709:Serum Albumin / Q000032:analysis; D012907:Smoking; D013053:Spectrophotometry; D017392:Thiobarbituric Acid Reactive Substances
D008076:Cholesterol, HDL; D016166:Free Radical Scavengers; D007202:Indicators and Reagents; D012709:Serum Albumin; D017392:Thiobarbituric Acid Reactive Substances; D008315:Malondialdehyde; D000080242:8-Hydroxy-2'-Deoxyguanosine; D043303:Aryldialkylphosphatase; C468406:PON1 protein, human; D003849:Deoxyguanosine