Leishmania chagasi: an ecto-3'-nucleotidase activity modulated by inorganic phosphate and its possible involvement in parasite-macrophage interaction.
In this work we showed that living cells of Leishmania chagasi was able to hydrolyse 3'AMP 10 times more than 5'AMP. When parasites were grown in a low phosphate concentration (2 mM) the cellular proliferation decreased by 50% compared to cells grown in the presence of a high phosphate concentration (80 mM). However, the ecto-3'nucleotidase activity was 2-fold higher when L. chagasi was grown in a low phosphate concentration. This modulation observed on ecto-3'nucleotidase activity was not observed on ecto-5'nucleotidase activity. These results suggest that low concentration of Pi in the culture medium modulates ecto-3'nucleotidase activity that may lead to modulation of important processes for the cell. Interestingly, the macrophage-parasite interaction increased by 45% when L. chagasi were grown at low phosphate concentration compared to the parasites grown in the presence of high phosphate source. Altogether, the results described here suggest that 3'nucleotidase activity modulated by external stimuli, Pi concentration, could be involved on parasite-macrophage interaction.
Instituto de Microbiologia Prof. Paulo de Góes, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, UFRJ, Cidade Universitária, Ilha do Fundão, Rio de Janeiro, R.J. 21941-590, Brazil.
Danielle P
DP
Vieira
Rafael
R
Paletta-Silva
Elvira M
EM
Saraiva
Angela H C S
AH
Lopes
José Roberto
JR
Meyer-Fernandes
D015720:5'-Nucleotidase / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D005260:Female; D054884:Host-Pathogen Interactions; D018314:Leishmania infantum / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development; D017737:Macrophages, Peritoneal / Q000469:parasitology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009708:Nucleotidases / Q000187:drug effects / Q000378:metabolism*; D010710:Phosphates / Q000494:pharmacology*
D010710:Phosphates; D009708:Nucleotidases; D015720:5'-Nucleotidase; C040470:3'-nucleotidase