Monitoring sleep and arousal in zebrafish.
Zebrafish has emerged in the past 5 years as a model for the study of sleep and wake behaviors. Experimental evidence has shown that periods of behavioral quiescence in zebrafish larvae and adults are sleep-like states, as these rest bouts are regulated by the circadian cycle, are associated with decreases in arousal, and are increased following rest deprivation. Furthermore, zebrafish share with mammals a hypocretin/orexin system that promotes wakefulness, and drugs that alter mammalian sleep have similar effects on zebrafish rest. In this chapter, we review the zebrafish sleep literature and describe a long-term, high-throughput monitoring system for observing sleep and wake behaviors in larval zebrafish.
Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts, USA.
Jason
J
Rihel
David A
DA
Prober
Alexander F
AF
Schier
D000818:Animals; D001143:Arousal; D012890:Sleep; D015027:Zebrafish / Q000196:embryology / Q000235:genetics / Q000502:physiology*