Increased expression of guanylate binding protein-1 in lesional skin of patients with cutaneous lupus erythematosus.
The large GTPase human guanylate binding protein-1 (GBP-1) is a key mediator of angiostatic effects of inflammation and is induced by interferon (IFN)-α and IFN-γ in endothelial cells (ECs). The aim of this study was to investigate whether GBP-1 is a marker of skin lesions in patients with cutaneous lupus erythematosus (CLE). Western blotting revealed that GBP-1 was in vitro induced by IFN-α and -γ in primary keratinocytes obtained from healthy controls. Moreover, we found that this protein was expressed by keratinocytes and ECs in primary and ultraviolet (UV)-induced skin lesions from patients with various subtypes of CLE, when compared to non-lesional skin. No GBP-1 expression was noted in skin biopsy specimens 24 or 72 h after UV irradiation prior to lesion formation in patients with CLE or in healthy control specimens with or without UV irradiation. Initial findings suggest that GBP-1 is not expressed in other skin diseases with different inflammatory aetiology, such as atopic dermatitis. We conclude that GBP-1 expression is closely associated with skin lesions in patients with CLE, suggesting a contribution of GBP-1 in the pathogenesis of this disease.
Division of Molecular and Experimental Surgery, University Medical Center Erlangen, Erlangen, Germany. elisabeth.naschberger@uk-erlangen.de
Elisabeth
E
Naschberger
Jörg
J
Wenzel
Cosima C
CC
Kretz
Martin
M
Herrmann
Michael
M
Stürzl
Annegret
A
Kuhn
D000328:Adult; D000368:Aged; D001706:Biopsy; D016022:Case-Control Studies; D054357:Chemokine CXCL10 / Q000378:metabolism; D054370:Chemokine CXCL9 / Q000378:metabolism; D005260:Female; D019204:GTP-Binding Proteins / Q000378:metabolism*; D006801:Humans; D007372:Interferons / Q000378:metabolism; D015603:Keratinocytes / Q000378:metabolism* / Q000473:pathology / Q000528:radiation effects; D008178:Lupus Erythematosus, Cutaneous / Q000378:metabolism* / Q000473:pathology / Q000532:radiotherapy; D008297:Male; D008875:Middle Aged; D064547:Myxovirus Resistance Proteins; D012867:Skin / Q000378:metabolism* / Q000473:pathology / Q000528:radiation effects; D014467:Ultraviolet Therapy
D054357:Chemokine CXCL10; D054370:Chemokine CXCL9; C490287:GBP1 protein, human; D064547:Myxovirus Resistance Proteins; D007372:Interferons; D019204:GTP-Binding Proteins