A new crystal form of human vascular adhesion protein 1.
Human vascular adhesion protein 1 (VAP-1) is involved in lymphocyte-endothelial cell adhesion and has been implicated in many human inflammatory diseases. VAP-1 is a member of the copper amine oxidase family of enzymes with a trihydroxyphenylalanine quinone (TPQ) cofactor. Previously characterized crystals of VAP-1 suffered from anisotropy and contained disordered regions; in addition, one form was consistently twinned. In an effort to grow crystals that diffracted to higher resolution for inhibitor-binding studies, a construct with an N-terminal deletion was made and expressed in the Chinese hamster ovary (CHO) glycosylation mutant cell line Lec8. Screening produced crystals that displayed some anisotropy and contained seven molecules per asymmetric unit. These crystals belonged to space group C2, with unit-cell parameters a=394.5, b=115.8, c=179.3 Å, β=112.3°. The structure was refined to a resolution of 2.9 Å, with Rcryst and Rfree values of 0.250 and 0.286, respectively.
School of Molecular Bioscience, University of Sydney, NSW 2006, Australia.
Karin
K
Ernberg
Aaron P
AP
McGrath
Thomas S
TS
Peat
Timothy E
TE
Adams
Xiaowen
X
Xiao
Tam
T
Pham
Janet
J
Newman
Ian A
IA
McDonald
Charles A
CA
Collyer
J Mitchell
JM
Guss
D006631:Amine Oxidase (Copper-Containing) / Q000737:chemistry*; D000818:Animals; D016466:CHO Cells; D020134:Catalytic Domain; D015815:Cell Adhesion Molecules / Q000737:chemistry*; D006224:Cricetinae; D003412:Cricetulus; D018360:Crystallography, X-Ray; D003545:Cysteine / Q000737:chemistry; D004220:Disulfides / Q000737:chemistry; D006031:Glycosylation; D006801:Humans; D008958:Models, Molecular; D011485:Protein Binding; D055503:Protein Multimerization
D015815:Cell Adhesion Molecules; D004220:Disulfides; C076668:AOC3 protein, human; D006631:Amine Oxidase (Copper-Containing); D003545:Cysteine