Screening systems for the identification of S-nitrosylated proteins.
S-nitrosylation is a well-characterized reaction involving the covalent binding of nitric oxide (NO) to cysteine residues (Cys) in a protein. Similar to protein phosphorylation, S-nitrosylation is a post-translational modification involved in the regulation of a large number of intracellular functions and signaling events. Moreover, like phosphorylation, S-nitrosylation is precisely regulated in time and space. A procedure known as the biotin-switch method that specifically detects S-nitrosylated proteins (SNO-P) was recently developed by Snyder's group. They found that many proteins are substrates for NO, and several groups have attempted to identify other SNO-P by improving this method. In this review, we describe the SNO-P identified using modified versions of the biotin-switch method.
Department of Medicinal Pharmacology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama 700-8530, Japan. uehara@pharm.okayama-u.ac.jp
Takashi
T
Uehara
Tadashi
T
Nishiya
D001665:Binding Sites; D001710:Biotin / Q000031:analogs & derivatives* / Q000378:metabolism; D019295:Computational Biology; D003545:Cysteine / Q000378:metabolism; D004220:Disulfides / Q000378:metabolism*; D008698:Mesylates / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D010084:Oxidation-Reduction; D040081:Protein Array Analysis / Q000379:methods; D011499:Protein Processing, Post-Translational; D011506:Proteins / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D026403:S-Nitrosothiols / Q000378:metabolism*; D013379:Substrate Specificity
D004220:Disulfides; D008698:Mesylates; C501660:N-(6-(biotinamido)hexyl)-3'-(2'-pyridyldithio)propionamide; D011506:Proteins; D026403:S-Nitrosothiols; D009569:Nitric Oxide; C074502:methanethiosulfonate; D001710:Biotin; D003545:Cysteine