Large scale enzymatic synthesis of oligosaccharides and a novel purification process.
Herein we report the practical chemo enzymatic synthesis of trisaccharide and derivatives of iGb3 and Gb3, and a novel purification process using immobilized yeast to remove the monosaccharide from the reaction mixture. High purity oligosaccharide compounds were achieved in large scale. This study represents a facile enzymatic synthesis of and novel purification process of oligosaccharide.
Department of Basic Science, Touro University-California, Vallejo, CA 94592, USA. guangyan.zhou@tu.edu
Guangyan
G
Zhou
Xianwei
X
Liu
Doris
D
Su
Lei
L
Li
Min
M
Xiao
Peng G
PG
Wang
D002852:Chromatography, Ion Exchange; D005915:Globosides / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification; D014281:Trihexosylceramides / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification; D014312:Trisaccharides / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification; D014534:UDPglucose 4-Epimerase / Q000378:metabolism*
D005915:Globosides; D014281:Trihexosylceramides; D014312:Trisaccharides; C514780:globotrihexosylceramide; C514781:isoglobotrihexosylceramide; D014534:UDPglucose 4-Epimerase; C020551:galactose epimerase