N-glycosylation at noncanonical Asn-X-Cys sequences in plant cells.
The vesicular transport pathway in plant cells is often used for higher accumulation of recombinant proteins. In the endoplasmic reticulum, which acts as a gateway to the vesicular transport pathway, N-glycosylation occurs on specific Asn residues. This N-glycosylation in recombinant proteins must be carefully regulated as it can impact their enzymatic activity, half lives in serum when injected, structural stability, etc. In eukaryotic cells, including plant cells, N-glycans were found to be attached to Asn residues in Asn-X-Ser/Thr (X ≠ Pro) sequences. However, recently, N-glycosylations at noncanonical Asn-X-Cys sequences have been found in mammals and yeast. Our laboratory has discovered that N-glycans are attached to Asn residues at Asn-Thr-Cys sequences of double-repeated B subunit of Shiga toxin 2e produced in plant cells, the first reported case of N-glycosylation at a noncanonical Asn-X-Cys sequence in plant cells.
Advanced Technology Research Laboratories, Idemitsu Kosan Co., Ltd., 1280 Kamiizumi, Sodegaura-shi, Chiba 299-0293, Japan.
Takeshi
T
Matsui
Eiji
E
Takita
Toshio
T
Sato
Satoko
S
Kinjo
Michie
M
Aizawa
Yukie
Y
Sugiura
Takashi
T
Hamabata
Kazutoshi
K
Sawada
Ko
K
Kato
D017360:Arabidopsis / Q000166:cytology*; D001216:Asparagine / Q000737:chemistry / Q000378:metabolism*; D003545:Cysteine / Q000737:chemistry / Q000378:metabolism*; D006031:Glycosylation; D018545:Lactuca / Q000166:cytology*; D059828:Plant Cells / Q000737:chemistry* / Q000378:metabolism*; D011134:Polysaccharides / Q000737:chemistry; D022641:Shiga Toxin 2 / Q000096:biosynthesis / Q000737:chemistry
D011134:Polysaccharides; D022641:Shiga Toxin 2; D001216:Asparagine; D003545:Cysteine