Sprayed cultured mucosal epithelial cell for deep dermal burns.
Mucosal epithelial cells have various advantages compared with epidermal cells, such as their high proliferation ability and long biologic activity. The objective of this study was to assess the clinical results after sprayed application of cultured mucosal epithelial autograft (CMEA) suspensions onto deep dermal burn wounds. Ten patients with deep dermal burns were included in a prospective study. The average total-body-surface-area burn was 17.7% (8%-45%). The average Abbreviated Burn Severity Index was 6.3 points (4-9 points). The application of sprayed CMEA suspension was performed onto an average body surface area of 2.05% (0.5%-5%; median, 2%). Eight patients were recruited for clinical follow-up after an average of 10 months (3-18 months). The average Vancouver Scar Scale score at follow-up was 1.5 points (range, 0-5 points). The average period of epithelialization in wound surface was 12.5 days. Our data show that enzymatic and careful surgical debridement and consecutive application of CMEA suspensions using a spray technique result in excellent cosmetic outcomes compared with any other methods.
Department of Oral and Maxillofacial Surgery, School of Medicine, Nagoya University, Nagoya, Japan. mueda@med.nagoya-u.ac.jp
Minoru
M
Ueda
D000293:Adolescent; D000328:Adult; D000336:Aerosols; D001830:Body Surface Area; D002056:Burns / Q000145:classification / Q000601:surgery*; D018929:Cell Culture Techniques; D049109:Cell Proliferation; D002921:Cicatrix / Q000473:pathology; D003646:Debridement; D062109:Dermatologic Surgical Procedures; D004847:Epithelial Cells / Q000637:transplantation*; D004848:Epithelium / Q000473:pathology / Q000503:physiopathology; D005260:Female; D015718:Fibrin Tissue Adhesive / Q000627:therapeutic use; D005500:Follow-Up Studies; D006801:Humans; D015601:Injury Severity Score; D008297:Male; D008875:Middle Aged; D009061:Mouth Mucosa / Q000166:cytology*; D011446:Prospective Studies; D012867:Skin / Q000473:pathology / Q000503:physiopathology; D013997:Time Factors; D014014:Tissue Adhesives / Q000627:therapeutic use; D023822:Tissue Engineering; D014182:Transplantation, Autologous; D016896:Treatment Outcome; D014945:Wound Healing / Q000502:physiology
D000336:Aerosols; D015718:Fibrin Tissue Adhesive; D014014:Tissue Adhesives