Immobilization of anti-CD31 antibody on electrospun poly(ɛ-caprolactone) scaffolds through hydrophobins for specific adhesion of endothelial cells.
Hydrophilicity improvement and bioactive surface design of poly(ɛ-caprolactone) (PCL) grafts are of key importance for their application in tissue engineering. Herein, we develop a convenient approach for achieving stable hydrophilic surfaces by modifying electrospun PCL grafts with a class II hydrophobin (HFBI) coating. Static water contact angles (WCA) demonstrated the conversion of the PCL grafts from hydrophobic to hydrophilic after the introduction of amphiphilic HFBI. ATR-FTIR and XPS confirmed the presence of self-assembled HFBI films on the surface of the PCL nanofibers. The biocompatibility of the HFBI-modified PCL grafts was evaluated by cell proliferation in vitro, and by arteriovenous shunt (AV shunt) experiments ex vivo. Anti-CD31 antibody, which is specific for endothelial cells (ECs), was subsequently immobilized on the HFBI-coated PCL scaffolds through protein-protein interactions. This bioactive PCL graft was found to promote the attachment and retention of endothelial cells. These results suggest that this stepwise strategy for introducing cell-specific binding molecules into PCL scaffolds may have potential for development of vascular grafts that can endothelialize rapidly in vivo.
State Key Laboratory of Elemento-Organic Chemistry, Nankai University, Tianjin, China.
Min
M
Zhang
Zhexiang
Z
Wang
Zefang
Z
Wang
Shuren
S
Feng
Haijin
H
Xu
Qiang
Q
Zhao
Shufang
S
Wang
Jianxin
J
Fang
Mingqiang
M
Qiao
Deling
D
Kong
D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry*; D002208:Caproates / Q000737:chemistry*; D002448:Cell Adhesion / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology* / Q000187:drug effects; D006801:Humans; D007783:Lactones / Q000737:chemistry*; D051379:Mice; D008954:Models, Biological; D041681:NIH 3T3 Cells; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000276:immunology*; D011108:Polymers / Q000737:chemistry* / Q000494:pharmacology*; D023822:Tissue Engineering
D000900:Anti-Bacterial Agents; D002208:Caproates; D007783:Lactones; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D011108:Polymers; C121056:caprolactone