Identification and functional characterization of a poly(A)-binding protein from Leishmania infantum (LiPABP).
Gene expression regulation in Leishmania has been related to post-transcriptional events involving mainly sequences present in the 5' and 3' untranslated regions. PABPs are high-affinity poly(A)-binding proteins that are implicated in the regulation of translation initiation, RNA stability and other important biological processes. We describe a PABP from Leishmania infantum (LiPABP) that shows a very high homology with PABPs from other eukaryotic organisms, including mammals and other parasites. LiPABP conserves the main domains present in other PABPs, maintains poly(A)-binding properties and is phosphorylated by p38 mitogen-activated protein kinase. Using the sera from dogs infected with L. infantum, we demonstrate that LiPABP is expressed in L. infantum promastigotes.
Servicio de Bioquímica-Investigación, Hospital Ramón y Cajal, IRyCIS, Madrid, Spain.
Natalia
N
Guerra
María
M
Vega-Sendino
M Isabel
MI
Pérez-Morgado
Edurne
E
Ramos
Manuel
M
Soto
Víctor M
VM
Gonzalez
M Elena
ME
Martín
D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D003001:Cloning, Molecular; D016000:Cluster Analysis; D004285:Dogs; D057809:HEK293 Cells; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D018314:Leishmania infantum / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D008969:Molecular Sequence Data; D010766:Phosphorylation; D010802:Phylogeny; D011061:Poly A / Q000235:genetics / Q000378:metabolism; D039102:Poly(A)-Binding Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D015800:Protozoan Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
D007106:Immune Sera; D039102:Poly(A)-Binding Proteins; D015800:Protozoan Proteins; D011061:Poly A; D048051:p38 Mitogen-Activated Protein Kinases