Fabrication of lactobionic-loaded chitosan microcapsules as potential drug carriers targeting the liver.
This paper demonstrates a general approach for fabrication of lactobionic chitosan microcapsules using layer-by-layer assembly via click chemistry. Chitosan was selectively modified with either azide (CHI-Az) or alkyne (CHI-Alk) groups. The growth of the CHI-Az/CHI-Alk click multilayer was studied experimentally by multilayer assembly on planar supports. Linear buildup of the film was observed. The chitosan click capsules were also analyzed with confocal laser scanning microscopy and transmission electron microscopy. Capsules were found to have regular spherical shapes. In addition, (CHI-Az/CHI-Alk)-coated particles were modified with fluorescein isothiocyanate to ensure that the particles can be easily post-functionalized. Finally, lactobionic acid was conjugated onto the (CHI-Az/CHI-Alk)-coated particles and the lactobionic particles exhibited hepatoma cell (HepG2) targeting behavior.
Key Laboratory of Biomedical Polymers of Ministry of Education, Department of Chemistry, Wuhan University, Wuhan, People's Republic of China.
Jing
J
Zhang
Cao
C
Li
Zhi-Yuan
ZY
Xue
Hai-Wei
HW
Cheng
Fu-Wei
FW
Huang
Ren-Xi
RX
Zhuo
Xian-Zheng
XZ
Zhang
D000480:Alkynes / Q000737:chemistry; D001386:Azides / Q000737:chemistry; D002119:Calcium Carbonate / Q000494:pharmacology; D002214:Capsules; D002448:Cell Adhesion / Q000187:drug effects; D048271:Chitosan / Q000138:chemical synthesis / Q000737:chemistry*; D057930:Click Chemistry / Q000379:methods*; D004187:Disaccharides / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry*; D016650:Fluorescein-5-isothiocyanate / Q000378:metabolism; D056945:Hep G2 Cells; D006801:Humans; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D018613:Microscopy, Confocal; D046529:Microscopy, Electron, Transmission; D009928:Organ Specificity / Q000187:drug effects; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared
D000480:Alkynes; D001386:Azides; D002214:Capsules; D004187:Disaccharides; D004337:Drug Carriers; C005608:lactobionic acid; D048271:Chitosan; D002119:Calcium Carbonate; D016650:Fluorescein-5-isothiocyanate