Shrimp Pm-fortilin inhibits the expression of early and late genes of white spot syndrome virus (WSSV) in an insect cell model.
Fortilin plays an important role in anti-apoptotic mechanisms and cell proliferation in many eukaryotic organisms. This work confirmed previous reports that Sf9 can support the replication of white spot syndrome virus (WSSV) genomic material by using immunohistochemistry with a specific antibody to detect the immediate early gene 1 (ie1) and by amplification of WSSV DNA and mRNA products. Using this insect-cell model system, we show that overexpression of Pm-fortilin in Sf9 cells inhibited the expression of WSSV early genes and late genes (WSSV-DNA polymerase, VP15 and VP28) but not an immediate early gene ie1. This is the first time that an insect cell line has been used to demonstrate interaction between a shrimp gene and genes of a shrimp virus.
Center for Genomics and Bioinformatics Research, Faculty of Science, Prince of Songkla University, Songkhla 90112, Thailand.
Benjamas
B
Nupan
Amornrat
A
Phongdara
Manasawan
M
Saengsakda
Jiann Horng
JH
Leu
Chu Fang
CF
Lo
D000818:Animals; D017781:Genes, Immediate-Early; D007313:Insecta / Q000166:cytology; D033561:Penaeidae / Q000276:immunology* / Q000821:virology*; D011506:Proteins / Q000235:genetics / Q000276:immunology; D046848:White spot syndrome virus 1
D011506:Proteins