Aldehyde-functionalized, water-soluble poly(para-phenylene): synthesis and streptavidin assay using FRET.
We have attempted to synthesize water-soluble poly(para-phenylene) derivative, poly{[2,5-bis(3-sulfonatobutoxy)-1,4-phenylene sodium salt]-alt-(1,4-phenylene)} (PPP-SO3). Aldehyde groups, versatile functional intermediate groups for immobilization of biomolecules, were introduced at both ends of PPP-SO3 chain to produce PPP-SO3-CHO. PPP-SO3-CHO showed good solubility in aqueous solution and blue emission color, which was expected as an energy donor in FRET mechanism. Biotin was attached to the polymer end groups via imine linkage to use as a ligand for streptavidin immobilization. The biotin coupled with polymer chain enables the polymer to bind with FITC-streptavidin leading to energy transfer from the blue-emitting polymer to green-emitting FITC via FRET.
Organic and Optoelectronic Materials Laboratory, Department of Advanced Organic Materials and Textile System Engineering, Chungnam National University, Daejeon 305-764, Korea.
Chan Kyu
CK
Kwak
Dong Myung
DM
Kim
Chang-Soo
CS
Lee
Minjung
M
Lee
Taek Seung
TS
Lee
D000447:Aldehydes / Q000737:chemistry*; D001710:Biotin / Q000737:chemistry / Q000378:metabolism; D003516:Cycloparaffins / Q000138:chemical synthesis* / Q000737:chemistry; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D055767:Immobilized Proteins / Q000737:chemistry / Q000378:metabolism; D019906:Nuclear Magnetic Resonance, Biomolecular; D010636:Phenols / Q000138:chemical synthesis* / Q000737:chemistry; D011108:Polymers / Q000138:chemical synthesis* / Q000737:chemistry; D011485:Protein Binding; D012995:Solubility; D013050:Spectrometry, Fluorescence; D019809:Streptavidin / Q000737:chemistry / Q000378:metabolism; D014867:Water / Q000737:chemistry
D000447:Aldehydes; D003516:Cycloparaffins; D055767:Immobilized Proteins; D010636:Phenols; D011108:Polymers; C509547:poly-para-phenylene; D014867:Water; D001710:Biotin; D019809:Streptavidin