Imaging cell movements in egg-cylinder stage mouse embryos.
Cell movements in the pregastrulation egg cylinder mouse embryo play an important role in patterning. The stereotypic movement of the anterior visceral endoderm converts a proximal-distal axis to an anteroposterior axis by properly positioning the primitive streak. The epiblast at this stage is also characterized by a great deal of cell mixing, about which very little is known. Visualizing such cell movements can help us understand their role in embryonic development. This protocol describes a method to isolate and culture the egg cylinder-stage mouse embryo, as well as an approach for time-lapse imaging of embryos cultured in vivo.
Shankar
S
Srinivas
D000818:Animals; D002465:Cell Movement; D015509:Developmental Biology / Q000379:methods*; D004622:Embryo, Mammalian / Q000166:cytology*; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D051379:Mice; D018715:Microscopy, Video / Q000379:methods*; D014481:United States