[Preparation of vero cell-adapted influenza H5N1 virus strain by genetic reassortment].
OBJECTIVE
To prepare Vero cell-adapted influenza H5N1 virus strain by Genetic Reassortment and produce influenza H5N1 vaccine using Vero cell as a substrate.


METHODS
Embryonated specific pathogen-free (SPF) hen's eggs and Vero cells were co-infected with Vero cell-adapted influenza virus A/Yunnan/1/2005 Va(H3N2) and A/Anhui/1/2005 (H5N1) via reverse genetics. The reassortant was screened with goat antibody against strain A/Yunnan/1/2005 Va(H3N2) and identified for subtype by hemagglutination-inhibition (HI) assays and gene analysis of HA and NA.


RESULTS
A Vero cell-adapted influenza H5N1 virus strain was obtained, and there was no significant difference in serum antibody titers of monovalent inactivated vaccine reassorted before and after (F = 0.857, P > 0.05).


CONCLUSIONS
The Vero cell-adapted influenza virus of epidemic strain may be reassortment between Vero cell-adapted and epidemic strains.
Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming.
Juan
J
Shen
Ming-Bo
MB
Sun
Lei
L
Ma
Shao-Hui
SH
Song
Wei-Dong
WD
Li
Shu-De
SD
Jiang
Jing-Si
JS
Yang
Guo-Yang
GY
Liao
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D002642:Chick Embryo; D002522:Chlorocebus aethiops; D053124:Influenza A Virus, H5N1 Subtype / Q000235:genetics / Q000276:immunology*; D007252:Influenza Vaccines / Q000235:genetics / Q000276:immunology; D011995:Recombination, Genetic; D014709:Vero Cells
D000914:Antibodies, Viral; D007252:Influenza Vaccines