Visual observation of avidin-biotin affinity by fluorescent G4.5 poly(amidoamine) dendrimer.
An air-treated G4.5 poly(amidoamine) (PAMAM) dendrimer displayed the enhanced fluorescence enough to be utilized as a fluorescence marker to visualize avidin-biotin affinity: On a fluorescence microscopic image, the avidin labeled by a fluorescent G4.5 PAMAM dendrimer was observed to be selectively bound on the biotin pattern that was prepared by amide-bonding of biotin on a carboxylic acid-terminated self-assembled monolayer and in turn by UV-irradiation with a photomask on the monolayer.
Graduate School of Science and Technology, Keio University, Hiyoshi, Yokohama 223-8522, Japan.
Govindachetty
G
Saravanan
Kenji
K
Daigo
Toyoko
T
Imae
Takao
T
Hamakubo
D001360:Avidin / Q000378:metabolism*; D001710:Biotin / Q000378:metabolism*; D050091:Dendrimers / Q000378:metabolism*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013194:Staining and Labeling
D050091:Dendrimers; D001360:Avidin; D001710:Biotin