Molecular typing of canine parvovirus variants by polymerase chain reaction and restriction enzyme analysis.
Canine parvovirus type 2 (CPV-2) is a pathogen of dogs, which causes acute gastroenteritis and lymphopenia mostly in young pups. This paper reports the incidence of CPV-2 infection in diarrhoeic dogs with an aim to define the involvement of various variants of canine parvovirus circulating in India. CPV-2a, a variant of CPV-2 was differentiated from CPV-2b by polymerase chain reaction (PCR). The samples positive for CPV-2b were further analysed by PCR and restriction endonuclease (RE) analysis using Mbo II to detect the CPV-2c variant. Of 129 faecal samples studied, 78 were found positive for canine parvovirus by PCR. Among the 78 samples, 27 were of CPV-2a, 39 of CPV-2b and 12 of CPV-2c type, respectively. This study also showed that CPV-2c, anew variant, is circulating in India. The CPV-2c could be successfully detected by PCR and RE analysis while CPV-2b is the major antigenic type prevalent in this region followed by CPV-2a.
Virus Laboratory, Centre for Animal Disease Research and Diagnosis (CADRAD), Indian Veterinary Research Institute (IVRI), Izatnagar, UP, India.
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Kumar
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Nandi
D000818:Animals; D017931:DNA Primers; D004283:Dog Diseases / Q000175:diagnosis* / Q000821:virology*; D004285:Dogs; D005243:Feces / Q000821:virology; D007194:India; D010322:Parvoviridae Infections / Q000175:diagnosis*; D017993:Parvovirus, Canine / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000662:veterinary; D015183:Restriction Mapping / Q000662:veterinary
D017931:DNA Primers