Kinetics studies of p-cresol biodegradation by using Pseudomonas putida in batch reactor and in continuous bioreactor packed with calcium alginate beads.
Present study deals with the biodegradation of p-cresol by using Pseudomonas putida in a batch reactor and a continuous bioreactor packed with calcium alginate beads. The maximum specific growth rate of 0.8121 h(-1) was obtained at 200 mg L(-1) concentration of p-cresol in batch reactor. The maximum p-cresol degradation rate was obtained 6.598 mg L(-1) h(-1) at S(o)=200 mg L(-1) and 62.8 mg L(-1) h(-1) at S(o)=500 mg L(-1) for batch reactor and a continuous bioreactor, respectively. The p-cresol degradation rate of continuous bioreactor was 9 to 10-fold higher than those of the batch reactor. It shows that the continuous bioreactor could tolerate a higher concentration of p-cresol. A Haldane model was also used for p-cresol inhibition in batch reactor and a modified equation similar to Haldane model for continuous bioreactor. The Haldane parameters were obtained as µ(max) 0.3398 h(-1), K(s) 110.9574 mg L(-1), and K(I) 497.6169 mg L(-1) in batch reactor. The parameters used in continuous bioreactor were obtained as D(max) 91.801 mg L(-1) h(-1), K(s) 131.292 mg L(-1), and K(I) 1217.7 mg L(-1). The value K(I) of continuous bioreactor is approximately 2.5 times higher than the batch reactor. Higher K(I) value of continuous bioreactor indicates P. putida can grow at high range of p-cresol concentration. The ability of tolerance of higher p-cresol concentrations may be one reason for biofilm attachment on the packed bed in the continuous operation.
Motilal Nehru National Institute of Technology, Allahabad 211004, India. anilmathur@mnnit.ac.in
A K
AK
Mathur
Shashi
S
Bala
C B
CB
Majumder
S
S
Sarkar
D000464:Alginates; D001673:Biodegradation, Environmental; D019149:Bioreactors; D003408:Cresols / Q000737:chemistry / Q000378:metabolism*; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008954:Models, Biological; D016958:Pseudomonas putida / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000737:chemistry / Q000378:metabolism
D000464:Alginates; D003408:Cresols; D006603:Hexuronic Acids; D014874:Water Pollutants, Chemical; C032538:4-cresol; D020723:Glucuronic Acid