A two-photon excited luminescence of water-soluble rhodamine-platinum(II) complex: fluorescent probe specific for Hg2+ detection in live cell.
The first example of cyclometalated platinum(II)-containing rhodamine probe (1) with two-photon induced luminescent properties was synthesized and investigated for mercury detection. A highly selective color change of 1, from light yellow to pink, is observed only in the presence of Hg(2+) due to the formation of 1,3,4-oxadiazole ring in 2. This selectivity of Hg(2+) with color changes can be observed easily by the naked-eye. Meanwhile, a remarkable turn-on and selective 20-fold fluorescent enhancement of 1 upon binding with Hg(2+) over the other tested metal ions was observed. The water-soluble probe 1 was successfully applied in the visualizing of the site of Hg(2+) accumulation as well as estimating of trace amounts of mercury ions in live HeLa cells by two-photon microscopy.
Department of Chemistry, Korea University, Seoul 136-701, Republic of Korea.
Jun Feng
JF
Zhang
Chang Su
CS
Lim
Bong Rae
BR
Cho
Jong Seung
JS
Kim
D005456:Fluorescent Dyes / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D007477:Ions; D008628:Mercury / Q000032:analysis / Q000737:chemistry*; D008670:Metals / Q000737:chemistry; D008856:Microscopy, Fluorescence / Q000379:methods; D008956:Models, Chemical; D010984:Platinum / Q000737:chemistry*; D012235:Rhodamines / Q000737:chemistry*; D012995:Solubility; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D014466:Ultraviolet Rays; D014867:Water / Q000737:chemistry*
D005456:Fluorescent Dyes; D007477:Ions; D008670:Metals; D012235:Rhodamines; D014867:Water; D010984:Platinum; D008628:Mercury