Deep-sequencing identification of the genomic targets of the cytidine deaminase AID and its cofactor RPA in B lymphocytes.
The cytidine deaminase AID hypermutates immunoglobulin genes but can also target oncogenes, leading to tumorigenesis. The extent of AID's promiscuity and its predilection for immunoglobulin genes are unknown. We report here that AID interacted broadly with promoter-proximal sequences associated with stalled polymerases and chromatin-activating marks. In contrast, genomic occupancy of replication protein A (RPA), an AID cofactor, was restricted to immunoglobulin genes. The recruitment of RPA to the immunoglobulin loci was facilitated by phosphorylation of AID at Ser38 and Thr140. We propose that stalled polymerases recruit AID, thereby resulting in low frequencies of hypermutation across the B cell genome. Efficient hypermutation and switch recombination required AID phosphorylation and correlated with recruitment of RPA. Our findings provide a rationale for the oncogenic role of AID in B cell malignancy.
Genomics & Immunity, National Institute of Arthritis, Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, Maryland, USA.
Arito
A
Yamane
Wolfgang
W
Resch
Nan
N
Kuo
Stefan
S
Kuchen
Zhiyu
Z
Li
Hong-wei
HW
Sun
Davide F
DF
Robbiani
Kevin
K
McBride
Michel C
MC
Nussenzweig
Rafael
R
Casellas
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000276:immunology; D002478:Cells, Cultured; D042002:Chromatin Assembly and Disassembly / Q000235:genetics; D003564:Cytidine Deaminase / Q000235:genetics / Q000378:metabolism*; D005803:Genes, Immunoglobulin / Q000235:genetics; D016259:Genes, myc / Q000235:genetics; D059014:High-Throughput Nucleotide Sequencing; D017578:Immunoglobulin Class Switching; D015847:Interleukin-4 / Q000276:immunology / Q000378:metabolism; D008070:Lipopolysaccharides / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D011401:Promoter Regions, Genetic / Q000235:genetics; D051716:Replication Protein A / Q000235:genetics / Q000378:metabolism*; D027041:Somatic Hypermutation, Immunoglobulin
D008070:Lipopolysaccharides; D051716:Replication Protein A; D015847:Interleukin-4; D003564:Cytidine Deaminase