Comparison of PCR with culture applied on nasopharyngeal and throat swab specimens for the detection of Bordetella pertussis.
An in-house nested polymerase chain reaction (PCR) was prospectively compared with culture for Bordetella pertussis detection in 435 nasopharyngeal and/or throat swabs from 304 patients. One hundred specimens - 21% of nasopharyngeal swabs and 25% of throat swabs - were PCR- and/or culture-positive. Seventy percent of positive nasopharyngeal samples and 44% of positive throat samples were culture-positive.
Department of Microbiology, Oslo University Hospital, Ullevål, Norway. mona.holberg-petersen@uus.no
Mona
M
Holberg-Petersen
Pål A
PA
Jenum
Turid
T
Mannsåker
Kjetil K
KK
Melby
D000293:Adolescent; D000328:Adult; D000368:Aged; D001431:Bacteriological Techniques / Q000379:methods*; D001886:Bordetella pertussis / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D002648:Child; D002675:Child, Preschool; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008875:Middle Aged; D009305:Nasopharynx / Q000382:microbiology*; D010614:Pharynx / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D055815:Young Adult