Leptotene/zygotene chromosome movement via the SUN/KASH protein bridge in Caenorhabditis elegans.
The Caenorhabditis elegans inner nuclear envelope protein matefin/SUN-1 plays a conserved, pivotal role in the process of genome haploidization. CHK-2-dependent phosphorylation of SUN-1 regulates homologous chromosome pairing and interhomolog recombination in Caenorhabditis elegans. Using time-lapse microscopy, we characterized the movement of matefin/SUN-1::GFP aggregates (the equivalent of chromosomal attachment plaques) and showed that the dynamics of matefin/SUN-1 aggregates remained unchanged throughout leptonene/zygotene, despite the progression of pairing. Movement of SUN-1 aggregates correlated with chromatin polarization. We also analyzed the requirements for the formation of movement-competent matefin/SUN-1 aggregates in the context of chromosome structure and found that chromosome axes were required to produce wild-type numbers of attachment plaques. Abrogation of synapsis led to a deceleration of SUN-1 aggregate movement. Analysis of matefin/SUN-1 in a double-strand break deficient mutant revealed that repair intermediates influenced matefin/SUN-1 aggregate dynamics. Investigation of movement in meiotic regulator mutants substantiated that proper orchestration of the meiotic program and effective repair of DNA double-strand breaks were necessary for the wild-type behavior of matefin/SUN-1 aggregates.
Department of Chromosome Biology, Max F. Perutz Laboratories, University of Vienna, Vienna, Austria.
Antoine
A
Baudrimont
Alexandra
A
Penkner
Alexander
A
Woglar
Thomas
T
Machacek
Christina
C
Wegrostek
Jiradet
J
Gloggnitzer
Alexandra
A
Fridkin
Franz
F
Klein
Yosef
Y
Gruenbaum
Pawel
P
Pasierbek
Verena
V
Jantsch
D000818:Animals; D017173:Caenorhabditis elegans / Q000166:cytology* / Q000378:metabolism*; D029742:Caenorhabditis elegans Proteins / Q000737:chemistry / Q000378:metabolism*; D018797:Cell Cycle Proteins / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D002843:Chromatin / Q000378:metabolism; D002875:Chromosomes / Q000378:metabolism*; D003599:Cytoskeleton / Q000378:metabolism; D053903:DNA Breaks, Double-Stranded; D005838:Genotype; D049469:Meiotic Prophase I; D008938:Mitosis; D008954:Models, Biological; D009687:Nuclear Proteins / Q000378:metabolism*; D020836:Protein Structure, Quaternary; D021381:Protein Transport; D018160:Receptors, Cytoplasmic and Nuclear / Q000737:chemistry / Q000378:metabolism*; D013573:Synaptonemal Complex / Q000378:metabolism
D029742:Caenorhabditis elegans Proteins; D018797:Cell Cycle Proteins; D002843:Chromatin; C544270:KDP-1 protein, C elegans; D009687:Nuclear Proteins; D018160:Receptors, Cytoplasmic and Nuclear; C514114:sun-1 protein, C elegans