In vitro analysis of nanoparticle uptake by macrophages using chemiluminescence.
This chapter provides a protocol for qualitative evaluation of nanoparticle internalization by phagocytic cells such as macrophages. This protocol uses luminol chemiluminescence to detect nanoparticle uptake. This protocol provides a preliminary qualitative look at phagocytosis which should be confirmed by other techniques such as electron microscopy, confocal microscopy, or as applicable to a given nanoparticle sample.
Nanotechnology Characterization Laboratory, Advanced Technology Program, SAIC-Frederick, Inc., National Cancer Institute at Frederick, Frederick, MD, USA.
Sarah L
SL
Skoczen
Timothy M
TM
Potter
Marina A
MA
Dobrovolskaia
D045744:Cell Line, Tumor; D018922:HL-60 Cells; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute / Q000473:pathology; D049408:Luminescent Agents / Q000737:chemistry; D008163:Luminescent Measurements / Q000379:methods*; D008165:Luminol / Q000737:chemistry; D008264:Macrophages / Q000187:drug effects* / Q000502:physiology; D018613:Microscopy, Confocal / Q000379:methods; D008854:Microscopy, Electron / Q000379:methods; D053758:Nanoparticles / Q000633:toxicity*; D010587:Phagocytosis / Q000187:drug effects* / Q000502:physiology
D049408:Luminescent Agents; D008165:Luminol