A novel real-time PCR method based on signaling-by-incorporation.
We describe a novel real-time PCR method, which utilizes the in situ generated signal upon incorporation of a fluorogenic nucleotide. The method has been compared with a conventional method and evaluated for practical uses such as quantification of cDNA and malaria diagnosis.
Biomedical Research Center, Korea Institute of Science and Technology, PO Box 131, Cheongryang, Seoul 130-650, South Korea.
Da-Rae
DR
Kim
Hee-Chul
HC
Ahn
Won-Ja
WJ
Lee
Dae-Ro
DR
Ahn
D003848:Deoxyguanine Nucleotides / Q000737:chemistry*; D005461:Fluorine / Q000737:chemistry*; D006801:Humans; D008288:Malaria / Q000175:diagnosis; D010966:Plasmodium vivax / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D012337:RNA, Ribosomal, 18S / Q000378:metabolism
D003848:Deoxyguanine Nucleotides; D012337:RNA, Ribosomal, 18S; D005461:Fluorine; C029603:deoxyguanosine triphosphate