[Expression and clinical significance of IL-17 and IL-21 in patients with acute leukemia].
This study was aimed to investigate the effects of peripheral blood Th17 cells, IL-17 and IL-21 in the occurrence and development of acute leukemia. 60 patients with acute leukemia (19 patients with ALL, 41 patients with AML) were divided into non-remission group (group A, n=24), remission group (group B, n=36); 25 healthy volunteers were used as control group (group C). In addition to this, these 60 patients were divided into infection group (n=32) and non-infection group (n=28) on the basis of infection status. The concentration of IL-17 and IL-21 in the peripheral blood mononuclear cell culture supernatant after stimulation with anti-CD3 and anti-CD28 mAb were determined with ELISA. The expression of CD4+ IL-17+ cells was determined by flow cytometry. The results showed that (1) the concentrations of IL-17 and IL-21, and proportion of Th17 cells in group A and group B were much lower than those in group C (p<0.05); (2) the expression levels of IL-17 and IL-21, and the proportion of Th17 cells in group A were lower than those in group B (p<0.05); (3) the expression levels of Th17 and IL-17 in infection group were lower than those in non-infection group (p<0.05). It is concluded that Th17 cells may play important roles in the occurrence and development of acute leukemia through secreting IL-17 and IL-21, and their functional level can partially reflect the status of leukemia and can be used to evaluate the risks of infection in patients with leukemia.
Department of Hematology, Huanggang Central Hospital, Huanggang 438000, Hubei Province, China.
Mei-Fang
MF
Su
Chang-Feng
CF
Wang
Yong-Mei
YM
Zhao
Ji-Xiong
JX
Wu
Yao
Y
Zhang
D000293:Adolescent; D000328:Adult; D015496:CD4-Positive T-Lymphocytes; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D020381:Interleukin-17 / Q000378:metabolism*; D007378:Interleukins / Q000378:metabolism*; D015470:Leukemia, Myeloid, Acute / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000378:metabolism*; D058504:Th17 Cells / Q000378:metabolism; D055815:Young Adult; D000098546:Interleukin-21
D020381:Interleukin-17; D007378:Interleukins; D000098546:Interleukin-21