Canine adenoviruses elicit both humoral and cell-mediated immune responses against rabies following immunisation of sheep.
Safe and efficient vaccination is important for rabies prevention in domestic animals. Replicative vectors expressing the rabies virus glycoprotein, derived from canine adenovirus have been reported to be promising vaccines in various animal models. In this paper we compare the potential of a replicative and a non-replicative vector, both based on canine adenovirus type 2 and expressing the rabies glycoprotein. Upon inoculation in sheep, immune responses against the rabies virus protein elicited by recombinant vectors were monitored. All immunised sheep produced a rapid and potent neutralizing antibody response against rabies virus after a single inoculation of either replicative or non-replicative recombinant canine adenovirus type 2. In addition, the non-replicative vector expressing the rabies glycoprotein stimulated antigen-specific CD4(+) and CD8(+) lymphocyte proliferation as well as IFN-γ production. These results suggest that vectors derived from canine adenovirus 2 could be considered for the development of promising vaccines in the ruminant species.
UMR 1161 Virologie, ANSES INRA ENVA, Maisons-Alfort, France.
Coraline
C
Bouet-Cararo
Vanessa
V
Contreras
Annie
A
Fournier
Corinne
C
Jallet
Jean Michel
JM
Guibert
Eric
E
Dubois
Richard
R
Thiery
Emmanuel
E
Bréard
Noël
N
Tordo
Jennifer
J
Richardson
Isabelle
I
Schwartz-Cornil
Stéphan
S
Zientara
Bernard
B
Klonjkowski
D006516:Adenoviruses, Canine / Q000235:genetics*; D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D049109:Cell Proliferation; D004337:Drug Carriers; D005822:Genetic Vectors; D007371:Interferon-gamma / Q000378:metabolism; D008297:Male; D011818:Rabies / Q000517:prevention & control*; D011819:Rabies Vaccines / Q000235:genetics / Q000276:immunology*; D011820:Rabies virus / Q000235:genetics / Q000276:immunology*; D012756:Sheep; D014613:Vaccines, Attenuated / Q000235:genetics / Q000276:immunology; D015164:Vaccines, Inactivated / Q000235:genetics / Q000276:immunology; D014614:Vaccines, Synthetic / Q000235:genetics / Q000276:immunology
D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D004337:Drug Carriers; D011819:Rabies Vaccines; D014613:Vaccines, Attenuated; D015164:Vaccines, Inactivated; D014614:Vaccines, Synthetic; D007371:Interferon-gamma