[Immunochemical assay of MIC A production by tumor cells in vitro and in vivo as a component of antitumor vaccine development].
Due to immunological monitoring, most of aggressive tumor cells are selected capable of producing soluble factors of immunosuppression in their microenvironment. A ligand for NK- and T-cells receptor (MICA/B) activation is one of them. We investigated MICA concentrations in serum of 10 healthy volunteers and 114 patients with solid tumors (breast cancer - 24, gastrointestinal tumors - 27, renal cell carcinoma - 15, lung cancer - 9, ovarian carcinoma - 13, cutaneous melanoma - 18, primary multiple tumors - 2, prostate cancer - 6). The lowest level of MICA was reported in ovarian carcinoma (91.19 +/- 29.42 pg/ml), the highest - in rectal cancer (311.13 +/- 50.11 pg/ml) while mean concentration in healthy volunteers was (19.38 +/- 5.91 pg/ml). Cells of melanoma, renal cell carcinoma and prostate cancer became capable of producing MICA during cultivation and antitumor vaccine preparation. It is suggested that culture supernatants be tested for MICA contents as well as patients screened for vaccination. Selection of patients should be carried out on the basis of MICA molecules detection in blood flow.
A B
AB
Danilova
A O
AO
Danilov
O L
OL
Fakhrumdinova
I A
IA
Badueva
V M
VM
Moiseenko
D000328:Adult; D000368:Aged; D019496:Cancer Vaccines / Q000276:immunology*; D002292:Carcinoma, Renal Cell / Q000276:immunology; D015195:Drug Design; D005260:Female; D015395:Histocompatibility Antigens Class I / Q000032:analysis* / Q000096:biosynthesis; D006801:Humans; D007120:Immunochemistry; D007680:Kidney Neoplasms / Q000276:immunology; D008297:Male; D008545:Melanoma / Q000276:immunology; D008875:Middle Aged; D009369:Neoplasms / Q000276:immunology* / Q000517:prevention & control*; D010051:Ovarian Neoplasms / Q000276:immunology; D018579:Patient Selection; D011471:Prostatic Neoplasms / Q000276:immunology; D011948:Receptors, Antigen, T-Cell; D055607:Receptors, Natural Killer Cell; D012004:Rectal Neoplasms / Q000276:immunology
D019496:Cancer Vaccines; D015395:Histocompatibility Antigens Class I; C099187:MHC class I-related chain A; D011948:Receptors, Antigen, T-Cell; D055607:Receptors, Natural Killer Cell