Induction of c-fos transcription in the medaka brain (Oryzias latipes) in response to mating stimuli.
Immediate-early genes (IEGs) are useful for mapping active brain regions in various vertebrates. Here we identified a c-fos homologue gene in medaka and demonstrated that the amounts of c-fos transcripts and proteins in the medaka brain increased in relation to an artificially evoked seizure, suggesting that the homologue gene has the characteristics of IEGs, which are used as markers of neural activity. Next, quantitative reverse-transcription-polymerase chain reaction revealed that female mating behaviors upregulated c-fos transcription in some brain regions including the telencephalon, optic tectum, and cerebellum. In addition, we performed in situ hybridization with a c-fos intron probe to detect the de novo synthesis of c-fos transcripts and confirmed induction of c-fos transcription in these brain regions after mating. This is the first report of IEG induction in response to mating stimuli in teleost fish. Our results indicated that c-fos expression was induced in response to behavioral stimuli in the medaka brain and that medaka c-fos could be a useful marker of neural activity.
Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan.
Teruhiro
T
Okuyama
Yuji
Y
Suehiro
Haruka
H
Imada
Atsuko
A
Shimada
Kiyoshi
K
Naruse
Hiroyuki
H
Takeda
Takeo
T
Kubo
Hideaki
H
Takeuchi
D000818:Animals; D001921:Brain / Q000378:metabolism / Q000502:physiology*; D005260:Female; D017781:Genes, Immediate-Early; D005819:Genetic Markers; D017403:In Situ Hybridization; D009990:Oryzias / Q000235:genetics / Q000502:physiology*; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics*; D012726:Sexual Behavior, Animal; D014158:Transcription, Genetic
D005819:Genetic Markers; D016760:Proto-Oncogene Proteins c-fos