Humoral and in vivo cellular immunity against the raw insect-derived recombinant Leishmania infantum antigens KMPII, TRYP, LACK, and papLe22 in dogs from an endemic area.
Leishmania infantum causes visceral leishmaniasis, a severe zoonotic and systemic disease that is fatal if left untreated. Identification of the antigens involved in Leishmania-specific protective immune response is a research priority for the development of effective control measures. For this purpose, we evaluated, in 27 dogs from an enzootic zone, specific humoral and cellular immune response by delayed-type hypersensitivity (DTH) skin test both against total L. infantum antigen and the raw Trichoplusia ni insect-derived kinetoplastid membrane protein-11 (rKMPII), tryparedoxin peroxidase (rTRYP), Leishmania homologue of receptors for activated C kinase (rLACK), and 22-kDa potentially aggravating protein of Leishmania (rpapLe22) antigens from this parasite. rTRYP induced the highest number of positive DTH responses (55% of leishmanin skin test [LST]-positive dogs), showing that TRYP antigen is an important T cell immunogen, and it could be a promising vaccine candidate against this disease. When TRYP-DTH and KMPII-DTH tests were evaluated in parallel, 82% of LST-positive dogs were detected, suggesting that both antigens could be considered as components of a standardized DTH immunodiagnostic tool for dogs.
Unitat de Farmacologia Veterinària and LeishLAB-Servei d'Anàlisi de Fàrmacs, Departament de Farmacologia, de Terapèutica i de Toxicologia, Universitat Autònoma de Barcelona, Barcelona, Spain. Felicitat.Todoli@uab.cat
Felicitat
F
Todolí
Laia
L
Solano-Gallego
Rafael
R
de Juan
Pere
P
Morell
Maria Del Carmen
Mdel C
Núñez
Rodrigo
R
Lasa
Silvia
S
Gómez-Sebastián
José M
JM
Escribano
Jordi
J
Alberola
Alhelí
A
Rodríguez-Cortés
D000818:Animals; D000953:Antigens, Protozoan / Q000276:immunology* / Q000378:metabolism; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006968:Hypersensitivity, Delayed; D056724:Immunity, Humoral; D018314:Leishmania infantum / Q000378:metabolism*; D007898:Leishmaniasis, Visceral / Q000662:veterinary*; D008297:Male; D008565:Membrane Proteins / Q000276:immunology* / Q000378:metabolism; D015800:Protozoan Proteins / Q000276:immunology* / Q000378:metabolism
D000953:Antigens, Protozoan; D008565:Membrane Proteins; D015800:Protozoan Proteins; C032127:leishmanin; C093139:LACK antigen, Leishmania