Morphologic changes in the tumor and liver in mice with transplanted RLS40 lymphosarcoma during increase of its drug resistance.
The progress of drug resistance of RLS(40) resistant lymphosarcoma and specific features of toxic lesions in the liver in polychemotherapy were studied. After intramuscular injection of tumor cells, the mice received a course of polychemotherapy. The tumor material was then collected and transplanted to intact animals, after which polychemotherapy was carried out. A total of 4 passages of tumor cells and 4 polychemotherapy courses were carried out. The expression of mdr1b, bcl-2, and p53 genes in tumor cells increased by 1.3, 2.3, and 1.6 times after 4 courses of polychemotherapy in comparison with intact tumor. Volume density of apoptoses in tumor tissue after 4 polychemotherapy courses decreased 1.7 times compared to that after single course. The increase in cytostatic load was associated with aggravation of destructive changes in the liver: the volume density of necroses in the liver increased 1.3 times after 4 passages of the tumor.
Center of Clinical and Experimental Medicine, Siberian Division of Russian Academy of Medical Sciences, Novosibirsk, Russia. alsenko@mail.ru
A V
AV
Sen'kova
T A
TA
Ageeva
M A
MA
Zenkova
D018435:ATP Binding Cassette Transporter, Subfamily B / Q000378:metabolism; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D017931:DNA Primers / Q000235:genetics; D003720:Densitometry; D018432:Drug Resistance, Multiple; D019008:Drug Resistance, Neoplasm; D004359:Drug Therapy, Combination / Q000009:adverse effects*; D019450:Genes, MDR / Q000235:genetics; D008099:Liver / Q000187:drug effects / Q000473:pathology*; D008228:Lymphoma, Non-Hodgkin / Q000188:drug therapy / Q000473:pathology*; D008297:Male; D051379:Mice; D009336:Necrosis; D016133:Polymerase Chain Reaction; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D016378:Tissue Transplantation; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D000097202:ATP-Binding Cassette Sub-Family B Member 4
D018435:ATP Binding Cassette Transporter, Subfamily B; D017931:DNA Primers; D019253:Proto-Oncogene Proteins c-bcl-2; D016159:Tumor Suppressor Protein p53; D000097202:ATP-Binding Cassette Sub-Family B Member 4