[Experimental research on anti-respiratory syncytial virus effect in vitro of earthworm coelomic fluid].
OBJECTIVE
To explore the antivirus function in vitro of earthworm coelomic fluid (ECF) by researching its effect on inhibiting respiratory syncytial virus (RSV).


METHODS
By the method of Hep-2 cell culture and using ribavirin as a positive control, the anti-RSV effect of ECF was investigated by observing cytopathic effect (CPE) with MTT colorimetric assay.


RESULTS
In Hep-2 cells, the CC50 of ECF and ribavirin were 3.11 mg/ml and 1.35 mg/ml separately. In the experiment of ECF directly killing RSV, the IC50 of ECF was 184.1 microg/ml, SI was 16.87; In the experiment of ECF preventing RSV invasion, no antiviral function of ECF within the experimental concentration range was observed; In the experiment of ECF inhibiting RSV replication, the IC50 of ECF was 1555. 8 microg/ml, SI was 1.99.


CONCLUSIONS
ECF couldn't prevent virus from invading into host cell, but showed direct killing-virus function and inhibition of the virus replication.
Department of Biochemistry and Molecular Biology, Shanxi Medical University, Taiyuan 030001, China.
Fang
F
Li
Jian-Hua
JH
Wang
Jian-Lin
JL
Zhang
Ru-Gang
RG
Zhong
Bo
B
Niu
D000818:Animals; D000998:Antiviral Agents / Q000737:chemistry / Q000494:pharmacology*; D002460:Cell Line; D006801:Humans; D020128:Inhibitory Concentration 50; D009835:Oligochaeta / Q000737:chemistry*; D012136:Respiratory Syncytial Viruses / Q000187:drug effects*; D014779:Virus Replication / Q000187:drug effects
D000998:Antiviral Agents