Axotomy induced changes in neuronal plasticity of sympathetic chain ganglia (SChG) neurons supplying descending colon in the pig.
Sympathetic neurons are capable of extensive regeneration following axonal injury. To investigate the response to axotomy of colon-projecting neurons (CPN) localized in the porcine sympathetic chain ganglia (SChG), the retrograde Fast Blue (FB) tracer, axonal transection and double immunohistochemistry methods were applied. The CPN were localized exclusively in the lumbar SChG and displayed a predominantly catecholaminergic [i.e. Tyrosine Hydroxylase (TH)/Dopamine β Hydroxylase (DβH)] and Neuropeptide Y (NPY) positive phenotype under physiological conditions. Axotomy led to a significant decrease in TH/DβH production and a simultaneous increase in the neuropeptides Galanin (GAL) and Somatostatin (SOM), but not NPY or Vasoactive Intestinal Peptide (VIP) expression in retrogradely traced perikarya. Furthermore, the decrease in density of TH-/DβH-, VIP-, Leu(5)-Enkephalin (LENK)-, Choline Acetyltransferase (ChAT)-immunoreactive (-IR) nerve fibers occurred after axotomy. These data suggest a species-specific response to axonal damage of the CPN localized in porcine SChG. Since the SChG neurons supervise the vasculature of gut both in physiological and pathological conditions, and since pig is a more accurate animal model of human gut than a rodent (Swindle et al., 1992), these data may contribute to the understanding of the pathology of several gut illnesses, like Crohn Disease and Irritable Bowel Syndrome which commonly affect western populations.
Division of Clinical Physiology, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, 13 Oczapowskiego Street, 10-719 Olsztyn, Poland. czaker@interia.pl
Cezary
C
Skobowiat
Jaroslaw
J
Calka
Mariusz
M
Majewski
D000818:Animals; D019771:Axotomy; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D044683:Colon, Descending / Q000294:innervation* / Q000378:metabolism; D004299:Dopamine beta-Hydroxylase / Q000378:metabolism; D019004:Galanin / Q000378:metabolism; D005728:Ganglia, Sympathetic / Q000502:physiology*; D023421:Models, Animal; D009412:Nerve Fibers / Q000378:metabolism; D009473:Neuronal Plasticity / Q000502:physiology*; D009478:Neuropeptide Y / Q000378:metabolism; D011897:Random Allocation; D013004:Somatostatin / Q000378:metabolism; D034421:Sus scrofa; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism
D009478:Neuropeptide Y; D014660:Vasoactive Intestinal Peptide; D013004:Somatostatin; D019004:Galanin; D014446:Tyrosine 3-Monooxygenase; D004299:Dopamine beta-Hydroxylase; D002795:Choline O-Acetyltransferase