Cloning and expression analysis of a G-protein α subunit--Gαo in the rice water weevil Lissorhoptrus oryzophilus Kuschel.
The open reading frame (ORF) encoding a novel G protein α subunit, Lo Gα(o), was cloned from the parthenogenetic rice water weevil, Lissorhoptrus oryzophilus Kuschel (Coleoptera: Curculionidae). The Lo Gα(o) ORF encodes a protein of 354 amino acid residues. The deduced protein sequence shares high homology with Gα(o) from other species. The expression patterns of Lo Gα(o) in various adult tissues were indicated by real-time quantitative PCR and Western blot. The results showed that Lo Gα(o) mRNA was expressed at similar levels in tissues except relative high levels in the antennae of adult, and Lo Gα(o) protein of an apparent molecular mass of about 40 kDa was expressed in various tissues of the adult. Immunocytochemical localization showed that Lo Gα(o) was mainly expressed in the dendrites of the trichoid sensilla in the antenna of the weevil. The tissue and cellular localization of Lo Gα(o) suggests that Lo Gα(o) may take a part in signal transduction of olfactory/gustatory.
State Key Laboratory of Rice Biology, Zhejiang University, Hangzhou, Zhejiang, China.
Gui-Juan
GJ
Kang
Zhong-Jun
ZJ
Gong
Jia-An
JA
Cheng
Zeng-Rong
ZR
Zhu
Cun-Gui
CG
Mao
D000595:Amino Acid Sequence; D000818:Animals; D057506:Arthropod Antennae / Q000378:metabolism; D015153:Blotting, Western; D003001:Cloning, Molecular; D044385:GTP-Binding Protein alpha Subunits / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D019476:Insect Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D055696:Olfactory Perception / Q000502:physiology; D009928:Organ Specificity; D016133:Polymerase Chain Reaction; D058618:Sensilla / Q000378:metabolism; D015398:Signal Transduction; D055697:Taste Perception / Q000502:physiology; D048889:Weevils / Q000235:genetics* / Q000378:metabolism
D044385:GTP-Binding Protein alpha Subunits; D019476:Insect Proteins