Comparison between DAX-8 and C-18 solid phase extraction of rainwater dissolved organic matter.
Rainwater is a very low concentrated matrix and, for dissolved organic matter (DOM) characterization, an efficient extraction procedure is essential. Isolation procedures based on the adsorption onto XAD-8 and C-18 sorbents have been used in the literature for rainwater DOM isolation, but a comparison between these procedures is lacking. In this work, UV-visible and molecular fluorescence spectroscopies highlighted differences between rainwater DOM isolated by DAX-8 (replacement for XAD-8) and by C-18. It was possible to recover higher rainwater DOM percentage by the C-18 based procedure than by the DAX-8 one. Rainwater protein-like compounds were better concentrated by the C-18 procedure than by the DAX-8 one, while humic-like compounds were similarly concentrated by both procedures. Furthermore, rainwater DOM extracted by the C-18 procedure was more representative of the global matrix, while DAX-8 preferentially extracted humic-like compounds.
CESAM (Centre for Environmental and Marine Studies) & Department of Chemistry, University of Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal.
Patrícia S M
PS
Santos
Marta
M
Otero
Olga M S
OM
Filipe
Eduarda B H
EB
Santos
Armando C
AC
Duarte
D000327:Adsorption; D004784:Environmental Monitoring / Q000379:methods*; D004867:Equipment Design; D006801:Humans; D006812:Humic Substances / Q000032:analysis; D011891:Rain; D052616:Solid Phase Extraction / Q000295:instrumentation* / Q000379:methods*; D013050:Spectrometry, Fluorescence / Q000379:methods; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D014867:Water / Q000737:chemistry; D014874:Water Pollutants, Chemical / Q000032:analysis*; D018508:Water Purification / Q000379:methods
D006812:Humic Substances; D014874:Water Pollutants, Chemical; D014867:Water