Cryptosporidium parvum causes gastroenteritis epidemics in the Nablus region of Palestine.
A total of 30 faecal samples collected from individuals admitted to a local hospital in Nablus city in Palestine with gastroenteritis symptoms, plus five faecal samples from healthy individuals living in the same area were screened for the presence of Cryptosporidium spp. by microscopic analysis using malachite green negative staining. Molecular techniques were used to confirm the microscopic identification. All 30 samples from individuals with gastroenteritis symptoms were positive by both techniques. No other parasites were found in the faecal material of patients or healthy individuals. To explore the source of the outbreak, water was collected from various reservoirs and springs that supply the city with drinking water. Al-Qaryoon water spring was found to be contaminated with Cryptosporidium using both microscopic and molecular analysis. No other water resources were found to be contaminated. Genotyping analysis of Cryptosporidium oocysts using PCR-RFLP technique identified the parasite as C. parvum.
Faculty of Medicine, Genetics Laboratory, An-Najah National University, Nablus, Palestine. ashussein@najah.edu
Ayman S
AS
Hussein
D003457:Cryptosporidiosis / Q000453:epidemiology*; D016785:Cryptosporidium parvum / Q000302:isolation & purification*; D016054:DNA, Protozoan / Q000302:isolation & purification; D058872:Epidemics; D005243:Feces / Q000469:parasitology; D005759:Gastroenteritis / Q000453:epidemiology* / Q000469:parasitology*; D006801:Humans; D008877:Middle East / Q000453:epidemiology; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D014867:Water / Q000469:parasitology; D014881:Water Supply
D016054:DNA, Protozoan; D014867:Water