Supercritical carbon dioxide extraction of ethyl p-methoxycinnamate from Kaempferia galanga L. rhizome and its apoptotic induction in human HepG2 cells.
In this study, supercritical carbon dioxide extraction of ethyl p-methoxycinnamate from Kaempferia galanga L. rhizome and its apoptotic induction in human HepG2 cells are reported for the first time. By using supercritical carbon dioxide extraction, the yield of ethyl p-methoxycinnamate identified by gas chromatography mass spectrometry (GC-MS) was as high as 2.5% with respect to the raw materials. In the anticancer assay, it was found that ethyl p-methoxycinnamate could inhibit the proliferation of the human hepatocellular liver carcinoma HepG2 cell line in a dose-dependent manner and induce the significant increase of the subG0 cell population. After treatment with ethyl p-methoxycinnamate, phosphatidylserine of HepG2 cells could significantly translocate to the surface of the membrane. The increase of an early apoptotic population was observed by both annexin-fluorescein isothiocyanate (FITC) and propidium iodide (PI) staining. It was concluded that ethyl p-methoxycinnamate not only induced cells to enter into apoptosis, but also affected the progress of the cell cycle.
Henan Institute of Science and Technology, Xinxiang, China.
Benguo
B
Liu
Feng
F
Liu
Chungang
C
Chen
Han
H
Gao
D017209:Apoptosis / Q000187:drug effects*; D002245:Carbon Dioxide; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D025924:Chromatography, Supercritical Fluid; D002934:Cinnamates / Q000302:isolation & purification / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D016650:Fluorescein-5-isothiocyanate; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D010718:Phosphatidylserines / Q000378:metabolism; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D027343:Rhizome / Q000737:chemistry*; D030521:Zingiberaceae / Q000737:chemistry*
D002934:Cinnamates; D010718:Phosphatidylserines; D010936:Plant Extracts; C531364:ethyl 4-methoxycinnamate; D002245:Carbon Dioxide; D016650:Fluorescein-5-isothiocyanate