DNA-templated covalent coupling of G4 PAMAM dendrimers.
Generation-4 polyamidoamine (PAMAM) dendrimers were surface-functionalized with azides or alkynes and conjugated to one DNA strand. DNA-controlled self-assembly of alternating azide and alkyne dendrimers on a DNA template enabled the coupling of the dendrimers by the azide-alkyne "click" reaction to form covalently coupled dimers, trimers, and tetramers. Polymerization of the DNA-dendrimer conjugates was also demonstrated, as well as assembly in a circular structure on DNA origami and imaging by atomic force microscopy.
Centre for DNA Nanotechnology (CDNA) at The Interdisciplinary Nanoscience Center (iNANO), Aarhus University, DK-8000 Aarhus C, Denmark.
Huajie
H
Liu
Thomas
T
Tørring
Mingdong
M
Dong
Christian B
CB
Rosen
Flemming
F
Besenbacher
Kurt V
KV
Gothelf
D000480:Alkynes / Q000737:chemistry*; D001386:Azides / Q000737:chemistry*; D001710:Biotin / Q000737:chemistry; D057930:Click Chemistry / Q000379:methods*; D004247:DNA / Q000737:chemistry*; D050091:Dendrimers / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D036103:Nanotechnology / Q000379:methods*; D009757:Nylons / Q000737:chemistry*; D019809:Streptavidin / Q000737:chemistry
D000480:Alkynes; D001386:Azides; D050091:Dendrimers; D009757:Nylons; C520550:PAMAM-G4; D001710:Biotin; D004247:DNA; D019809:Streptavidin