SLC11A1 polymorphisms in inflammatory bowel disease and Mycobacterium avium subspecies paratuberculosis status.
OBJECTIVE
To test for association of SLC11A1 with inflammatory bowel disease (IBD) and Mycobacterium avium subspecies paratuberculosis (MAP) status in a Caucasian cohort.


METHODS
five hundred and seven Crohn's disease (CD) patients, 474 ulcerative colitis (UC) patients, and 569 healthy controls were genotyped for SLC11A1 1730G>A and SLC11A1 469+14G>C using pre-designed TaqMan SNP assays. χ(2) tests were applied to test for association of single nucleotide polymorphisms (SNPs) with disease, and the presence of MAP DNA.


RESULTS
SLC11A1 1730G>A and SLC11A 1469+14G>C were not associated with CD, UC, or IBD. The SLC11A1 1730A minor allele was over-represented in patients who did not require immunomodulator therapy (P = 0.002, OR: 0.29, 95% CI: 0.13-0.66). The frequency of the SLC11A1 469+14C allele was higher in the subset of study participants who tested positive for MAP DNA (P = 0.02, OR: 1.56, 95% CI: 1.06-2.29). No association of SLC11A1 1730G>A with MAP was observed.


CONCLUSIONS
Although SLC11A1 was not associated with IBD, association with MAP suggests that SLC11A1 is important in determining susceptibility to bacteria implicated in the etiology of CD.
Department of Paediatrics, University of Otago, PO Box 4345, Christchurch 8140, New Zealand. robert.bentley@otago.ac.nz.
Lucy C
LC
Stewart
Andrew S
AS
Day
John
J
Pearson
Murray L
ML
Barclay
Richard B
RB
Gearry
Rebecca L
RL
Roberts
Robert W
RW
Bentley
D016022:Case-Control Studies; D027682:Cation Transport Proteins / Q000235:genetics*; D016009:Chi-Square Distribution; D003093:Colitis, Ulcerative / Q000188:drug therapy / Q000208:ethnology / Q000235:genetics*; D003424:Crohn Disease / Q000188:drug therapy / Q000208:ethnology / Q000235:genetics* / Q000382:microbiology; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006801:Humans; D007155:Immunologic Factors / Q000627:therapeutic use; D016927:Mycobacterium avium subsp. paratuberculosis / Q000302:isolation & purification*; D009520:New Zealand; D016017:Odds Ratio; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D018570:Risk Assessment; D012307:Risk Factors; D044465:White People / Q000235:genetics; D000099173:Natural Resistance-associated Macrophage Protein 1
D027682:Cation Transport Proteins; D007155:Immunologic Factors; D000099173:Natural Resistance-associated Macrophage Protein 1