[Stimulatory effect of TNF-α on differentiation and maturation of dendritic cells].
The study was aimed to explore the stimulatory effect of tumor necrosis factorα (TNF-α) on differentiation and maturation of human peripheral blood mononuclear cell-derived dendritic cells (PBMNCDC). 30 ml of peripheral blood from healthy volunteers were collected, the PBMNC were isolated according to Thomas' method and were cultured with incubation of granulocyte/macrophage colony stimulating factor (GM-CSF), interleukin-4 (IL-4) for 8 day, and TNF-α was added on day 5. The expression of HLA-DR, CD83 and CD1a on surface of DC were detected by flow cytometry, the ability of DC to stimulate the proliferation of allogenic T cells was evaluated by MTT assay. The results showed that the TNF-α could up-regulate the expression of HLA-DR, CD83 and CD1a and activated DC could enhance the proliferation of allogenic T cells. Furthermore, the TNF-α at the concentration of 20 ng/ml was more effective. It is concluded that the TNF-α can effectively enhance the functional differentiation and maturation of the PBMNCDC and TNF-α at the concentration of 20 ng/ml is most effective to enhance differentiation and maturation of DC.
Department of Pediatrics, Peking Union Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China.
Ying
Y
Ru
Hong-Mei
HM
Song
Hang
H
Li
Wei
W
Cui
D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000166:cytology* / Q000187:drug effects*; D005434:Flow Cytometry; D006801:Humans; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D014409:Tumor Necrosis Factor-alpha