[Pirfenidone diminishes SiO2 induced lung fibrosis in rats].
OBJECTIVE
to investigate whether pirfenidone (PFD) presents the antifibrotic effect in silicosis of rats.


METHODS
SD rats were randomly divided into five groups: the non-treat group, the normal saline group, the normal saline + PFD group, the SiO2 group, the SiO2 + PFD group. Rats except in the non-treat group were intratracheally instilled with SiO2 (25 mg/ml) or normal saline. The rats in normal saline + PFD group and the SiO2 + PFD group were given PFD (50 mg/kg) orally the next day after instillation and throughout the study. Rats were respectively sacrificed 7, 21, 42 days after instillation. The pathology changes were evaluated by Haematoxylin-eosin (HE), Van Gieson and Foot staining, and the hydroxyproline (HYP) content of pulmonary tissue was determined.


RESULTS
compared with the SiO2 group, PFD could relieve the fibrotic changes in the lungs of rats. The fibrotic degree in silicotic lesions of lungs was lower in the SiO2 + PFD group than that of SiO2 group. The HYP content in the lungs of the SiO2 + PFD group [(0.75 ± 0.12) mg/g] was significantly lower than that of the SiO2 group [(1.19 ± 0.17) mg/g] at 42 days after instillation (P < 0.05).


CONCLUSIONS
these data support that PFD has an antifibrotic effect against SiO2 induced lung fibrosis in rats, Which appears to be changing collagen accumulation and inhibiting pulmonary fibrosis.
Institute of Hygiene, Zhejiang Academy of Medical Sciences, Hangzhou 310013, China.
Ling-Fang
LF
Feng
Zhen-Yu
ZY
Jia
Li-Jin
LJ
Zhu
Li
L
Ju
Jun-Qiang
JQ
Chen
Zhao-Qiang
ZQ
Jiang
Ri-Ping
RP
Chen
Zhen
Z
Ma
Xing
X
Zhang
D000818:Animals; D006909:Hydroxyproline / Q000378:metabolism; D008168:Lung / Q000187:drug effects* / Q000378:metabolism* / Q000473:pathology; D008297:Male; D011658:Pulmonary Fibrosis / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D011728:Pyridones / Q000494:pharmacology* / Q000627:therapeutic use; D051381:Rats; D017207:Rats, Sprague-Dawley; D012822:Silicon Dioxide / Q000009:adverse effects
D011728:Pyridones; D012822:Silicon Dioxide; C093844:pirfenidone; D006909:Hydroxyproline