Cell-based cytokine therapy for skin rejuvenation.
The interaction between stem cells from human exfoliated deciduous teeth (SHEDs)-derived growth factors and human dermal fibroblast (HDF) has been investigated for the first time, as the application of SHEDs for dermal wound healing remains speculative. Stem cells from human exfoliated deciduous teeth have effects on HDFs by increasing collagen synthesis and by activating proliferation and migration activity of HDFs, suggesting that SHEDs or SHED-derived conditioned medium (SH-CM) can be used for the treatment of photoaging. Our results suggest that SHEDs and SH-CM should be constitutionally suited for photoaging treatment. Mainly with secreted growth factors or extracellular matrix proteins, SHEDs contribute to enhanced wound-healing potential of HDFs. Further mechanism studies using neutralizing antibodies against each growth factor may clarify the role of soluble factors of SHEDs in wound-healing process.
Department of Oral and Maxillofacial Surgery, School of Medicine, Nagoya University, Nagoya Aichi, Japan. mueda@med.nagoya-u.ac.jp
Minoru
M
Ueda
Yudai
Y
Nishino
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D001854:Bone Marrow Cells / Q000502:physiology; D018929:Cell Culture Techniques; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D003094:Collagen / Q000096:biosynthesis / Q000187:drug effects; D024042:Collagen Type I / Q000032:analysis; D017077:Culture Media, Conditioned; D016207:Cytokines / Q000627:therapeutic use*; D003782:Dental Pulp / Q000166:cytology; D016326:Extracellular Matrix Proteins / Q000627:therapeutic use; D005260:Female; D005347:Fibroblasts / Q000187:drug effects / Q000528:radiation effects; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000627:therapeutic use; D020781:Matrix Metalloproteinase 1 / Q000032:analysis; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D051379:Mice; D008812:Mice, Hairless; D012060:Rejuvenation / Q000502:physiology*; D012867:Skin / Q000166:cytology / Q000528:radiation effects; D015595:Skin Aging / Q000473:pathology* / Q000528:radiation effects; D013997:Time Factors; D014094:Tooth, Deciduous / Q000166:cytology; D014466:Ultraviolet Rays; D014945:Wound Healing / Q000187:drug effects
D000954:Antigens, Surface; D024042:Collagen Type I; D017077:Culture Media, Conditioned; D016207:Cytokines; D016326:Extracellular Matrix Proteins; D036341:Intercellular Signaling Peptides and Proteins; C525591:STRO-1 antigen, human; D003094:Collagen; D020781:Matrix Metalloproteinase 1