A selective novel non-enzyme glucose amperometric biosensor based on lectin-sugar binding on thionine modified electrode.
A novel non-enzyme glucose amperometric biosensor was fabricated based on biospecific binding affinity of concanavalin A (Con A) for D-glucose on thionine (TH) modified electrode. TH can be covalently immobilized on potentiostatically activated glassy carbon electrode through Schiff-base reaction. Subsequently, the surface-adherent polydopamine film formed by self-polymerization of dopamine attached to TH and afforded binding sites for the subsequent immobilization of Con A molecules via Michael addition and/or Schiff-base reaction with high stability. Thus, a sensing platform for specific detection towards D-glucose was established. The binding of Con A towards D-glucose can be monitored through the decrease of the electrode response of the TH moiety. Due to the high affinity of Con A for D-glucose and high stability of the resulting sensing platform, the fabricated biosensor exhibited high selectivity, good sensitivity, and wide linear range from 1.0×10(-6) to 1.0×10(-4) M with a low detection limit of 7.5×10(-7) M towards D-glucose.
College of Chemistry, Chemical Engineering and Materials Science, Engineering Research Center of Pesticide and Medicine Intermediate Clean Production, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Normal University, 88 Wenhua East Road, Jinan 250014, People's Republic of China.
Feng
F
Li
Yan
Y
Feng
Limin
L
Yang
Liang
L
Li
Chenfei
C
Tang
Bo
B
Tang
D015374:Biosensing Techniques / Q000295:instrumentation*; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D003208:Concanavalin A / Q000737:chemistry*; D003217:Conductometry / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005947:Glucose / Q000032:analysis* / Q000737:chemistry; D010640:Phenothiazines / Q000737:chemistry*; D011485:Protein Binding
D020099:Coated Materials, Biocompatible; D010640:Phenothiazines; D003208:Concanavalin A; D005947:Glucose; C009469:thionine