Modification of a micellar system for amino acid separation by MEKC--application for amino acid profiling in formulations for parenteral use.
The paper proposes a new method for amino acid determination which can be applied for amino acid profiling in solutions for parenteral nutrition. The MEKC method based on a mixed micellar system was developed for the separation of 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) derivatized amino acids. Background electrolyte was based on tris-borate buffer with high alkaline pH. Sodium dodecyl sulfate micelles were modified using 1,2-hexanediol as a co-surfactant. The effect of the modifier on amino acid migration was studied with respect to hydrophobicity of the analytes. The modifier appeared to be suitable to improve the separation of AQC-tagged amino acids without an adverse effect on buffer ionic strength or EOF velocity. The method was successfully validated and applied for amino acid profiling in medicinal preparations for parenteral nutrition. The results obtained were compared with a reference chromatographic method (amino acid analyser).
Department of Biochemistry and Biopharmaceuticals, National Medicines Institute, Chełmska str. 30/34, 00-725 Warsaw, Poland. m-jaworska@il.waw.pl
Małgorzata
M
Jaworska
Zofia
Z
Szulińska
Małgorzata
M
Wilk
Elżbieta
E
Anuszewska
D000596:Amino Acids / Q000737:chemistry*; D000634:Aminoquinolines / Q000737:chemistry*; D002219:Carbamates / Q000737:chemistry*; D002623:Chemistry Techniques, Analytical; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002853:Chromatography, Liquid / Q000379:methods; D006018:Glycols / Q000737:chemistry; D006586:Hexanes; D006863:Hydrogen-Ion Concentration; D007263:Infusions, Parenteral / Q000379:methods*; D008823:Micelles; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D013388:Succinimides / Q000737:chemistry; D013997:Time Factors
C078178:6-aminoquinolyl-N-hydroxysuccinimidyl carbamate; D000596:Amino Acids; D000634:Aminoquinolines; D002219:Carbamates; D006018:Glycols; D006586:Hexanes; D008823:Micelles; D013388:Succinimides; D012967:Sodium Dodecyl Sulfate; C001426:N-hydroxysuccinimide; C119102:1,2-hexanediol