Discovery of putative oocyte quality markers by comparative ExacTag proteomics.
OBJECTIVE
Identification of the biomarkers of oocyte quality, and developmental and reprogramming potential is of importance to assisted reproductive technology in humans and animals.


METHODS
PerkinElmer ExacTag™ Kit was used to label differentially proteins in pig oocyte extracts (oocyte proteome) and pig oocyte-conditioned in vitro maturation media (oocyte secretome) obtained with high- and low-quality oocytes.


RESULTS
We identified 16 major proteins in the oocyte proteome that were expressed differentially in high- versus low-quality oocytes. More abundant proteins in the high-quality oocyte proteome included kelch-like ECH-associated protein 1 (an adaptor for ubiquitin-ligase CUL3), nuclear export factor CRM1 and ataxia-telangiectasia mutated protein kinase. Dystrophin (DMD) was more abundant in low-quality oocytes. In the secretome, we identified 110 proteins, including DMD and cystic fibrosis transmembrane conductance regulator, two proteins implicated in muscular dystrophy and cystic fibrosis, respectively. Monoubiquitin was identified in the low-quality-oocyte secretome.


CONCLUSIONS
A direct, quantitative proteomic analysis of small oocyte protein samples can identify potential markers of oocyte quality without the need for a large amount of total protein. This approach will be applied to discovery of non-invasive biomarkers of oocyte quality in assisted human reproduction and in large animal embryo transfer programs.
Morehouse School of Medicine, Atlanta, GA, USA.
Michael D
MD
Powell
Gaurishankar
G
Manandhar
Lee
L
Spate
Miriam
M
Sutovsky
Shawn
S
Zimmerman
Shrikesh C
SC
Sachdev
Mark
M
Hannink
Randall S
RS
Prather
Peter
P
Sutovsky
D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D009865:Oocytes / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D013552:Swine
D015415:Biomarkers; D020543:Proteome