[Association of cooking oil fumes exposure and oxidative DNA damage among occupational exposed populations].
BACKGROUND
Previous investigations indicate that cooks are exposed to polycyclic aromatic hydrocarbons (PAH) from cooking oil fumes (COF). However, Emission of PAH and their carcinogenic potencies from cooking oil fumes sources have not been investigated among cooks.


OBJECTIVE
To investigate the urinary excretion of a marker for oxidative DNA damage, 8-hydroxydeoxyguanosine (8-OHdG), in different groups of cooks and different exposure groups, and to study the association between 8-OHdG and 1-hydroxypyrene (1-OHP), a biological marker for PAH exposure.


METHODS
Urine samples were collected from different groups of cooks (n = 86) and from unexposed controls (n = 36), all are male with similar age and smoking habits. The health status, occupational history, smoking, and alcohol consumption 24 hours prior to sampling was estimated from questionnaires. The urinary samples were frozen for later analyses of 8-OHdG and 1-OHP by high performance liquid chromatography.


RESULTS
Excretion in urine of 8-OHdG were similar for controls (mean 1.2 µmol/mol creatinine, n = 36), and for those who had been in the kitchen room with exhaust hood operation (mean 1.5 µmol/mol creatinine, n = 45). COF exposed cooks without exhaust hood operation had increased excretion of 8-OHdG (mean 2.3 µmol/mol creatinine, n = 18). The difference between this group and the unexposed controls was significant. The urinary levels of ln 1-OHP and ln 8-OHdG were still significantly correlated in a multiple regression analysis.


CONCLUSIONS
Results indicate that exposure to PAH or possibly other compounds in COF may cause oxidative DNA damage.
Shenzhen Center for Diseases Control and Prevention, Shenzhen 518020, China.
Yue-bin
YB
Ke
Xin-yun
XY
Xu
Jian-hui
JH
Yuan
Shi-song
SS
Fang
Yi-min
YM
Liu
Tang-chun
TC
Wu
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D000395:Air Pollutants, Occupational / Q000652:urine*; D003296:Cooking; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000652:urine; D006801:Humans; D008297:Male; D016273:Occupational Exposure; D009821:Oils / Q000009:adverse effects; D018384:Oxidative Stress; D011084:Polycyclic Aromatic Hydrocarbons / Q000009:adverse effects*; D011795:Surveys and Questionnaires; D055815:Young Adult
D000395:Air Pollutants, Occupational; D009821:Oils; D011084:Polycyclic Aromatic Hydrocarbons; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine