Expression pattern of enkephalinergic neurons in the developing spinal cord revealed by preproenkephalin-green fluorescent protein transgenic mouse and its colocalization with GABA immunoreactivity.
To gain better insight into the ontogenic function of enkephalin (ENK) in the spinal cord, it is necessary to have a clear picture of the developing pattern of the ENKergic neurons. To address this question, we used transgenic mice which reveal ENKergic neurons easily by expressing enhanced green fluorescent protein (GFP) under the specific transcriptional control of the preproenkephalin (PPE) gene. GFP-positive neurons first appeared at embryonic day (E) 11.5 in the ventromedial part of the cervical ventral gray matter. At E13, they were mainly present in the intermediate zone. Thereafter, GFP-positive neurons increased progressively in number and extended from ventral to dorsal regions. Quantitative analysis showed that GFP-positive neurons peaked in number at postnatal day (P) 7 at the cervical level. The number of GFP-positive neurons reached a peak at P3 at the lumbar level. At P21, the distribution pattern of GFP immunoreactivity was similar to that in the adult spinal cord. Double-labeling results showed that about one-third of the total γ-aminobutyric acid cell population colocalized with GFP: 34.9 ± 3.5% at E16 and 32.4 ± 3.7% at P3. Double-labeled neurons accounted for nearly half of the GFP-positive neurons: 42.4 ± 2.4% at E16 and 44.1 ± 2.9% at P3. Taken together, the present results suggest that ENKergic neurons develop according to a rostrocaudal and ventrodorsal gradient. These results have broad implications for understanding the functional roles of ENKergic neurotransmission in the developing spinal cord.
Department of Anatomy, Histology and Embryology, K.K. Leung Brain Research Center, Fourth Military Medical University, Xi'an, PR China.
Jing
J
Huang
Jing
J
Chen
Wen
W
Wang
Ya Yun
YY
Wang
Wei
W
Wang
Yan Yan
YY
Wei
Ri Yi
RY
Shi
Takeshi
T
Kaneko
Yun Qing
YQ
Li
Sheng Xi
SX
Wu
D000818:Animals; D004745:Enkephalins / Q000235:genetics* / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D049452:Green Fluorescent Proteins / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D011498:Protein Precursors / Q000235:genetics* / Q000378:metabolism; D021381:Protein Transport; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013116:Spinal Cord / Q000166:cytology / Q000196:embryology* / Q000378:metabolism*; D013194:Staining and Labeling; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D004745:Enkephalins; D011498:Protein Precursors; D012333:RNA, Messenger; D049452:Green Fluorescent Proteins; D005680:gamma-Aminobutyric Acid; C033660:preproenkephalin