Long-term serum proteomes are quite similar under high- and low-flux hemodialysis treatment.
OBJECTIVE
Aim of the study was to identify long-term differences of middle and high-molecular-weight serum constituents under high- and low-flux hemodialysis treatments. Thus, the entire predialytic serum proteomes had to be analyzed using identical hemodialysis membrane material but with different cut-off values.


RESULTS
A cross-over study and a global native chromatographic proteomic approach were used to analyze serum compositions of 16 patients suffering from end-stage renal disease.


RESULTS
No significant or reproducible differences were found between predialytic serum samples from high- and low-flux dialysis treatments using UV-absorbance and fluorescence spectrometry, PMF, or sequence tags. In contrast, there are characteristic differences in the predialytic serum composition of the patients considered and two control sets, which include samples obtained post-dialytically from patients and samples from healthy controls. Only a fraction of β(2)-microglobulin, an example of so-called middle molecules, exhibits the expected molecular weight. A small fraction was found with high molecular weight unaffected by any dialysis treatment. Moreover, immunoreactivity of fragments of β(2)-microglobulin, surprisingly, was also not affected by the cut-off of dialysis membranes.


CONCLUSIONS
Thus, simply increasing the pore size of a hemodialysis membrane may not have any long-term effect on serum composition.
Institute of Biochemistry I, University Hospital, Jena, Germany.
Julia
J
Hallbauer
Stefan
S
Kreusch
Andree
A
Klemm
Gunter
G
Wolf
Heidrun
H
Rhode
D000368:Aged; D000369:Aged, 80 and over; D018592:Cross-Over Studies; D006801:Humans; D007676:Kidney Failure, Chronic / Q000097:blood*; D008567:Membranes, Artificial; D008875:Middle Aged; D020543:Proteome / Q000032:analysis*; D006435:Renal Dialysis / Q000295:instrumentation / Q000379:methods*; D014118:Toxins, Biological / Q000097:blood; D001613:beta 2-Microglobulin / Q000032:analysis
D008567:Membranes, Artificial; D020543:Proteome; D014118:Toxins, Biological; D001613:beta 2-Microglobulin; C026865:uremia middle molecule toxins