Levosimendan modulates programmed forms of cell death through K(ATP) channels and nitric oxide.
Levosimendan exerts cardioprotection through mitochondrial K(ATP) (mitoK(ATP)) channels opening. In addition, intracoronary levosimendan was found to modulate programmed forms of cell death by nitric oxide (NO) involvement. The aim of this study was to examine the role of mitoK(ATP) channels and NO in the effects of levosimendan on apoptosis/autophagy. In H9c2 cells treated with hydrogen peroxide apoptosis/autophagy, survival signaling, cell viability, mitochondrial membrane potential, and permeability transition pore opening were analyzed through Western blot and colorimetric and fluorescence assays. Pretreatment of H9c2 cells with levosimendan was able to counteract the oxidative injuries caused by hydrogen peroxide. The effects of levosimendan were potentiated by diazoxide and were similar to those elicited by the autophagic activator rapamycin. The autophagic inhibitor 3-methyladenine reduced the effects of levosimendan, whereas after the pan-caspases inhibitor N-Acetyl-Asp-Glu-Val-Asp-al (Z-VAD.FMK), cell survival and autophagy in response to levosimendan increased. Both the mitoK(ATP) channels inhibition and the NO synthase blocking attenuated the cardioprotection elicited by levosimendan. The results have shown that levosimendan protects H9c2 cells against oxidative injuries through the modulation of the interplay between autophagy and apoptosis and the activation of survival signaling. The mitoK(ATP) channels and NO may be involved in such cardioprotection through interference with mitochondrial functioning.
Laboratorio di Fisiologia, Dipartimento di Medicina Clinica e Sperimentale, Università degli Studi del Piemonte Orientale A. Avogadro, Via Solaroli 17 and Chirurgia Sperimentale, Azienda Ospedaliera Universitaria Maggiore della Carità, corso Mazzini 36, Novara, Italy.
Francesca
F
Uberti
Philippe P
PP
Caimmi
Claudio
C
Molinari
David
D
Mary
Giovanni
G
Vacca
Elena
E
Grossini
D000818:Animals; D016923:Cell Death / Q000187:drug effects / Q000502:physiology; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects* / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D006835:Hydrazones / Q000494:pharmacology*; D009569:Nitric Oxide / Q000502:physiology*; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology; D015221:Potassium Channels / Q000502:physiology*; D011724:Pyridazines / Q000494:pharmacology*; D051381:Rats; D000077464:Simendan
D006835:Hydrazones; D015221:Potassium Channels; D011724:Pyridazines; C117780:mitochondrial K(ATP) channel; D009569:Nitric Oxide; D000077464:Simendan