Gangliosides' analysis by MALDI-ion mobility MS.
The combination of ion mobility with matrix-assisted laser desorption/ionization allows for the rapid separation and analysis of biomolecules in complex mixtures (such as tissue sections and cellular extracts), as isobaric lipid, peptide, and oligonucleotide molecular ions are pre-separated in the mobility cell before mass analysis. In this study, MALDI-IM MS is used to analyze gangliosides, a class of complex glycosphingolipids that has different degrees of sialylation. Both GD1a and GD1b, structural isomers, were studied to see the effects on gas-phase structure depending upon the localization of the sialic acids. A total ganglioside extract from mouse brain was also analyzed to measure the effectiveness of ion mobility to separate out the different ganglioside species in a complex mixture.
NIDA IRP, NIH, Structural Biology Unit, Cellular Neurobiology Branch, 333 Cassell Drive, Baltimore, MD 21224, USA.
Shelley N
SN
Jackson
Benoit
B
Colsch
Thomas
T
Egan
Ernest K
EK
Lewis
J Albert
JA
Schultz
Amina S
AS
Woods
D000818:Animals; D001921:Brain / Q000378:metabolism*; D005732:Gangliosides / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D057230:Limit of Detection; D051379:Mice; D008810:Mice, Inbred C57BL; D015394:Molecular Structure; D012015:Reference Standards; D052616:Solid Phase Extraction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation / Q000379:methods*
D005732:Gangliosides