[Changes in the bacterial spectrum in severe burn wounds].
OBJECTIVE
To determine the time axis for increase in Gram negative bacterial strains in burn wounds during hospitalization.


METHODS
Retrospective.


METHODS
Eighty-five patients hospitalized at the Clinic of burns and reconstructive surgery between 2006 and 2008 were enrolled in the study. The major criteria for enrolment were more than 15% of total body surface area (TBSA) burned, hospital stay of 1 month or more and age over 18 years. Specimens for microbiological examination were collected on days 2, 6, 10, 14 and 20 after admission.


RESULTS
A total of 777 bacterial strains were isolated from the study patients, with 64.6% of these strains being Gram positive and 35.4% Gram negative. The most frequently isolated Gram positive pathogens were coagulase-negative Staphylococcus (260 strains) and Bacillus sp. (113 strains), while the most common Gram negative pathogens were Pseudomonas aeruginosa (81 strains), Escherichia coli (63 strains) and Acinetobacter calcoaceticus-baumannii complex (57 strains).


CONCLUSIONS
The study provided data that supports the assumption of increase in Gram negative bacterial strains in burn wounds during hospitalization. Nevertheless, even on day 20 after admission, such strains did not predominate.
Klinika popálenin a rekonstrukcní chirurgie FN Brno. b.lipovy@seznam.cz
B
B
Lipový
H
H
Rihová
M
M
Hanslianová
D
D
Burgetová
Y
Y
Kaloudová
I
I
Suchánek
P
P
Brychta
D000293:Adolescent; D000328:Adult; D000368:Aged; D001419:Bacteria / Q000302:isolation & purification*; D002056:Burns / Q000382:microbiology*; D005260:Female; D006090:Gram-Negative Bacteria / Q000302:isolation & purification; D006801:Humans; D008297:Male; D008875:Middle Aged; D055815:Young Adult