Usefulness of mycobacterial interspersed repetitive-unit locus PCR amplification in rapid diagnosis of Beijing lineage strain infection among pediatric tuberculosis patients.
We assessed the usefulness of mycobacterial interspersed repetitive-unit-variable-number tandem-repeat loci 26, 31, ETR-A, Mtub30, and Mtub02 and a deletion-targeted multiplex PCR in identifying pediatric Mycobacterium tuberculosis Beijing lineage strain infection. We found that the Beijing lineage isolates accounted for ∼ 62% (130/210) of the study isolates.
Department of Infectious Diseases and Gastroenterology, Children's Hospital of Chongqing Medical University, No. 136 Zhongshan Road #2, Yuzhong District, Chongqing 400014, People's Republic of China.
Ruixi
R
Liu
Linlin
L
Xing
Ze
Z
Peng
Yukun
Y
Zhang
Chaomin
C
Zhu
Zhenhua
Z
Yang
D001431:Bacteriological Techniques / Q000379:methods*; D002648:Child; D002675:Child, Preschool; D004269:DNA, Bacterial / Q000235:genetics; D006801:Humans; D020071:Interspersed Repetitive Sequences; D009169:Mycobacterium tuberculosis / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D014376:Tuberculosis / Q000175:diagnosis* / Q000382:microbiology*
D004269:DNA, Bacterial