1H, 13C and 15N NMR assignments of the C1A and C1B subdomains of PKC-delta.
The Protein Kinase C family of enzymes is a group of serine/threonine kinases that play central roles in cell-cycle regulation, development and cancer. A key step in the activation of PKC is translocation to membranes and binding of membrane-associated activators including diacylglycerol (DAG). Interaction of novel and conventional isotypes of PKC with DAG and phorbol esters occurs through the two C1 regulatory domains (C1A and C1B), which exhibit distinct ligand binding selectivity that likely controls enzyme activation by different co-activators. PKC has also been implicated in physiological responses to alcohol consumption and it has been proposed that PKCα (Slater et al. J Biol Chem 272(10):6167-6173, 1997; Slater et al. Biochemistry 43(23):7601-7609, 2004), PKCε (Das et al. Biochem J 421(3):405-413, 2009) and PKCδ (Das et al. J Biol Chem 279(36):37964-37972, 2004; Das et al. Protein Sci 15(9):2107-2119, 2006) contain specific alcohol-binding sites in their C1 domains. We are interested in understanding how ethanol affects signal transduction processes through its affects on the structure and function of the C1 domains of PKC. Here we present the (1)H, (15)N and (13)C NMR chemical shift assignments for the Rattus norvegicus PKCδ C1A and C1B proteins.
Department of Pharmacology, University of Colorado School of Medicine, Aurora, CO 80045, USA.
Brian P
BP
Ziemba
P Ziemba
PZ
Brian
Jamie C
JC
Booth
C Booth
CB
Jamie
David N M
DN
Jones
Jones N M
JN
David
D000595:Amino Acid Sequence; D000818:Animals; D002247:Carbon Isotopes / Q000737:chemistry; D004075:Diglycerides; D006859:Hydrogen / Q000737:chemistry; D008969:Molecular Sequence Data; D009587:Nitrogen Isotopes / Q000737:chemistry; D019906:Nuclear Magnetic Resonance, Biomolecular; D051745:Protein Kinase C-delta / Q000737:chemistry*; D017434:Protein Structure, Tertiary; D051381:Rats; D016415:Sequence Alignment
D002247:Carbon Isotopes; D004075:Diglycerides; D009587:Nitrogen Isotopes; D006859:Hydrogen; D051745:Protein Kinase C-delta