Toxic effects of domoic acid in the seabream Sparus aurata.
Neurotoxicity induced in fish by domoic acid (DA) was assessed with respect to occurrence of neurotoxic signs, lethality, and histopathology by light microscopy. Sparus aurata were exposed to a single dose of DA by intraperitoneal (i.p.) injection of 0, 0.45, 0.9, and 9.0 mg DA kg(-1) bw. Mortality (66.67 ± 16.67%) was only observed in dose of 9.0 mg kg(-1) bw. Signs of neurological toxicity were detected for the doses of 0.9 and 9.0 mg DA kg(-1) bw. Furthermore, the mean concentrations (±SD) of DA detected by HPLC-UV in extracts of brain after exposure to 9.0 mg DA kg(-1) bw were 0.61 ± 0.01, 0.96 ± 0.00, and 0.36 ± 0.01 mg DA kg(-1) tissue at 1, 2, and 4 hours. The lack of major permanent brain damage in S. aurata, and reversibility of neurotoxic signs, suggest that lower susceptibility to DA or neuronal recovery occurs in affected individuals.
CIIMAR/CIMAR, Centro Interdisciplinar de Investigação Marinha e Ambiental, Rua dos Bragas 177-289, 4150-123 Porto, Portugal. isabelcaldevilla@gmail.com
Isabel
I
Nogueira
Alexandre
A
Lobo-da-Cunha
António
A
Afonso
Socorro
S
Rivera
Joana
J
Azevedo
Rogério
R
Monteiro
Rosa
R
Cervantes
Ana
A
Gago-Martinez
Vítor
V
Vasconcelos
D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D002851:Chromatography, High Pressure Liquid; D005393:Fish Diseases / Q000378:metabolism / Q000473:pathology; D007608:Kainic Acid / Q000031:analogs & derivatives* / Q000633:toxicity; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D009474:Neurons / Q000187:drug effects*; D020258:Neurotoxicity Syndromes / Q000378:metabolism / Q000473:pathology / Q000662:veterinary*; D017470:Receptors, Glutamate / Q000378:metabolism; D021541:Sea Bream
D017470:Receptors, Glutamate; C012301:domoic acid; D007608:Kainic Acid