Preventive effects of autologous bone marrow mononuclear cell implantation on intrahepatic ischemic-type biliary lesion in rabbits.
BACKGROUND
The ischemic-type biliary lesion (ITBL) is one of the most serious biliary complications of liver transplantation. This study aimed to investigate the effects of autologous bone marrow mononuclear cell (BM-MNC) implantation on neovascularization and the prevention of intrahepatic ITBL in a rabbit model.


METHODS
The rabbits were divided into control, experimental model, and cell implantation groups, with 10 in each group. The model of intrahepatic ITBL was established by clamping the hepatic artery and common bile duct. Autologous BM-MNCs were isolated from the tibial plateau by density gradient centrifugation and were implanted through the common hepatic artery. Changes in such biochemical markers as aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, gamma-glutamyltranspeptidase, total bilirubin and direct bilirubin were measured. Four weeks after operation, cholangiography, histopathological manifestations, differentiation of BM-MNCs, microvessel density and the expression of vascular endothelial growth factor were assessed.


RESULTS
Compared with the experimental model group, the BM-MNC implantation group showed superiority in the time to recover normal biochemistry. The microvessel density and vascular endothelial growth factor expression of the implantation group were significantly higher than those of the control and experimental model groups. The ITBL in the experimental model group was more severe than that in the implantation group and fewer new capillary blood vessels occurred around it.


CONCLUSIONS
Implanted autologous BM-MNCs can differentiate into vascular endothelial cells, promote neovascularization and improve the blood supply to the ischemic bile duct, and this provides a new way to diminish or prevent intrahepatic ITBL after liver transplantation.
Department of Hepatobiliary and Pancreaticosplenic Surgery, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, China.
Zhao-Wei
ZW
Qu
Da-Zhi
DZ
Chen
Qin-Song
QS
Sheng
Ren
R
Lang
Qiang
Q
He
Ming-Feng
MF
Wang
D000818:Animals; D001653:Bile Ducts, Intrahepatic / Q000473:pathology*; D001854:Bone Marrow Cells / Q000166:cytology; D016026:Bone Marrow Transplantation / Q000379:methods*; D002454:Cell Differentiation / Q000502:physiology; D042783:Endothelial Cells / Q000166:cytology; D005260:Female; D007511:Ischemia / Q000473:pathology / Q000628:therapy*; D016031:Liver Transplantation / Q000009:adverse effects*; D008297:Male; D018919:Neovascularization, Physiologic / Q000502:physiology; D011183:Postoperative Complications / Q000473:pathology / Q000517:prevention & control / Q000628:therapy; D011817:Rabbits; D015996:Survival Rate; D014182:Transplantation, Autologous