Fine structure of granal thylakoid membrane organization using cryo electron tomography.
The architecture of grana membranes from spinach chloroplasts was studied by cryo electron tomography. Tomographic reconstructions of ice-embedded isolated grana stacks enabled to resolve features of photosystem II (PSII) in the native membrane and to assign the absolute orientation of individual membranes of granal thylakoid discs. Averaging of 3D sub-volumes containing PSII complexes provided a 3D structure of the PSII complex at 40 Å resolution. Comparison with a recently proposed pseudo-atomic model of the PSII supercomplex revealed the presence of unknown protein densities right on top of 4 light harvesting complex II (LHCII) trimers at the lumenal side of the membrane. The positions of individual dimeric PSII cores within an entire membrane layer indicates that about 23% supercomplexes must be of smaller size than full C(2)S(2)M(2) supercomplexes, to avoid overlap.
Electron microscopy group, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, The Netherlands. r.kouril@rug.nl
Roman
R
Kouřil
Gert T
GT
Oostergetel
Egbert J
EJ
Boekema
D002736:Chloroplasts / Q000648:ultrastructure*; D020285:Cryoelectron Microscopy; D045332:Photosystem II Protein Complex / Q000737:chemistry / Q000648:ultrastructure*; D010860:Pigments, Biological; D020524:Thylakoids / Q000737:chemistry / Q000648:ultrastructure*
D045332:Photosystem II Protein Complex; D010860:Pigments, Biological