Working with RNA.
Working with RNA is not a special discipline in molecular biology. However, RNA is chemically and structurally different from DNA and a few simple work rules have to be implemented to maintain the integrity of the RNA. Alkaline pH, high temperatures, and heavy metal ions should be avoided when possible and ribonucleases kept in check. The chapter outlines the specific precautions recommended for work with RNA and describes some of the modifications to standard protocols in molecular biology that are relevant to RNA work. The methods are applicable to all types of RNA and require a minimum of specialized equipment.
Department of Cellular and Molecular Medicine, The Panum Institute, University of Copenhagen, Copenhagen, Denmark. hamra@sund.ku.dk
Henrik
H
Nielsen
D002623:Chemistry Techniques, Analytical / Q000379:methods*; D004047:Diethyl Pyrocarbonate / Q000378:metabolism; D004586:Electrophoresis / Q000379:methods; D005821:Genetic Techniques; D006863:Hydrogen-Ion Concentration; D008670:Metals / Q000378:metabolism; D009711:Nucleotides / Q000378:metabolism; D011309:Preservation, Biological / Q000379:methods; D012313:RNA / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D012260:Ribonucleases / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013696:Temperature
D008670:Metals; D009711:Nucleotides; D012313:RNA; D012260:Ribonucleases; D004047:Diethyl Pyrocarbonate