A genomic perspective on the potential of Actinobacillus succinogenes for industrial succinate production.
BACKGROUND
Succinate is produced petrochemically from maleic anhydride to satisfy a small specialty chemical market. If succinate could be produced fermentatively at a price competitive with that of maleic anhydride, though, it could replace maleic anhydride as the precursor of many bulk chemicals, transforming a multi-billion dollar petrochemical market into one based on renewable resources. Actinobacillus succinogenes naturally converts sugars and CO2 into high concentrations of succinic acid as part of a mixed-acid fermentation. Efforts are ongoing to maximize carbon flux to succinate to achieve an industrial process.


RESULTS
Described here is the 2.3 Mb A. succinogenes genome sequence with emphasis on A. succinogenes's potential for genetic engineering, its metabolic attributes and capabilities, and its lack of pathogenicity. The genome sequence contains 1,690 DNA uptake signal sequence repeats and a nearly complete set of natural competence proteins, suggesting that A. succinogenes is capable of natural transformation. A. succinogenes lacks a complete tricarboxylic acid cycle as well as a glyoxylate pathway, and it appears to be able to transport and degrade about twenty different carbohydrates. The genomes of A. succinogenes and its closest known relative, Mannheimia succiniciproducens, were compared for the presence of known Pasteurellaceae virulence factors. Both species appear to lack the virulence traits of toxin production, sialic acid and choline incorporation into lipopolysaccharide, and utilization of hemoglobin and transferrin as iron sources. Perspectives are also given on the conservation of A. succinogenes genomic features in other sequenced Pasteurellaceae.


CONCLUSIONS
Both A. succinogenes and M. succiniciproducens genome sequences lack many of the virulence genes used by their pathogenic Pasteurellaceae relatives. The lack of pathogenicity of these two succinogens is an exciting prospect, because comparisons with pathogenic Pasteurellaceae could lead to a better understanding of Pasteurellaceae virulence. The fact that the A. succinogenes genome encodes uptake and degradation pathways for a variety of carbohydrates reflects the variety of carbohydrate substrates available in the rumen, A. succinogenes's natural habitat. It also suggests that many different carbon sources can be used as feedstock for succinate production by A. succinogenes.
Department of Microbiology and Molecular Genetics, 2215 Biomedical Biophysical Sciences building, Michigan State University, East Lansing, MI 48824, USA.
James B
JB
McKinlay
Maris
M
Laivenieks
Bryan D
BD
Schindler
Anastasia A
AA
McKinlay
Shivakumara
S
Siddaramappa
Jean F
JF
Challacombe
Stephen R
SR
Lowry
Alicia
A
Clum
Alla L
AL
Lapidus
Kirk B
KB
Burkhart
Victoria
V
Harkins
Claire
C
Vieille
D000188:Actinobacillus / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D002462:Cell Membrane / Q000378:metabolism; D016680:Genome, Bacterial / Q000235:genetics*; D007218:Industrial Microbiology; D007501:Iron / Q000378:metabolism; D053858:Metabolic Networks and Pathways / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D039002:Prophages / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D019802:Succinic Acid / Q000378:metabolism*; D014774:Virulence / Q000235:genetics
D001426:Bacterial Proteins; D012336:RNA, Ribosomal, 16S; D019802:Succinic Acid; D007501:Iron