Lipopolysaccharide promotes adhesion and migration of murine dental papilla-derived MDPC-23 cells via TLR4.
Odontoblasts and/or dental pulp cells are responsible for tooth repair and dentin formation. Furthermore, adhesion and migration are critical processes for tissue regeneration. This study was performed to clarify whether lipopolysaccharide (LPS) modulates adhesion and migration of the murine odontoblast-like cell line MDPC-23, and whether Toll-like receptor 4 (TLR4) signaling is engaged in this process. TLR4 expression in MDPC-23 cells was examined by RT-PCR. Adhesion assay was performed using type I collagen-coated plates. Migration ability was determined by a commercial assay kit. Phosphorylation of IκB-α, FAK, AKT, and ERK was examined by Western blot analysis. TLR4 was functionally expressed in MDPC-23 cells. LPS treatment enhanced adhesion and migration of MDPC-23 cells in a dose-dependent manner. Blockade of TLR4 using its antibody restored LPS-induced adhesion and migration of MDPC-23 cells. These findings indicate that LPS, an immune activator from Gram-negative bacteria, can promote the adhesion and migration ability of MDPC-23 cells via TLR4.
Department of Biochemistry, College of Medicine, Konyang University, Daejeon, Republic of Korea.
Jong-Hwan
JH
Park
Seong-Min
SM
Kwon
Hyo-Eun
HE
Yoon
Soo-A
SA
Kim
Sang-Gun
SG
Ahn
Jung-Hoon
JH
Yoon
D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects*; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects*; D003771:Dental Papilla / Q000166:cytology; D004789:Enzyme Activation / Q000187:drug effects; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D051417:Focal Adhesion Kinase 1 / Q000378:metabolism; D005786:Gene Expression Regulation; D008070:Lipopolysaccharides / Q000494:pharmacology*; D051379:Mice; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D051197:Toll-Like Receptor 4 / Q000235:genetics / Q000378:metabolism*
D008070:Lipopolysaccharides; D051197:Toll-Like Receptor 4; D051417:Focal Adhesion Kinase 1; D051057:Proto-Oncogene Proteins c-akt; D048049:Extracellular Signal-Regulated MAP Kinases