Electrochemical monitoring of chlorhexidine digluconate effect on polyelectrolyte immobilized bacteria and kinetic cell adhesion.
The electrochemical impedance spectroscopy (EIS) technique has been used as a sensitive method to explore the effect of antibacterial molecules on immobilized bacteria and biofilm formation. In this work, we describe the electrochemical spectroscopy as a powerful method to monitor the effect of chlorhexidine digluconate (CHX-Dg) on polyelectrolyte immobilized Escherichia coli K12 MG1655 and the kinetics of cell adhesion on gold electrodes. The experimental impedance data were modeled with a Zview program to find the best equivalent electrical circuit and analyse its parameter's properties. Polyelectrolyte multilayer formation on the electrode surface and bacteria immobilization greatly increased the electron-transfer resistance (R(et)) and reduced the constant phase element (CPE(dl)). The effect of CHX-Dg was studied in a 0.5 x 10⁻⁴ mmol l⁻¹ to 0.5 mmol l⁻¹ range. The relation between the evolution of R(et) and CHX-Dg concentration was found to be negatively correlated. When CHX-Dg was added, the electrochemical monitoring of the bacterial kinetic adhesion showed that the electrode's capacity (C(P)) variation remained stable, demonstrating that the addition of CHX-Dg in the broth inhibited bacterial adhesion.
Laboratoire de Biophysique, Faculté de Médecine de Monastir, 5019 Monastir, Tunisia. binal_borg@yahoo.fr
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Borghol
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Mora
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Sakly
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Lejeune
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Jouenne
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Jaffrézic-Renault
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Othmane
D002448:Cell Adhesion / Q000187:drug effects*; D018914:Cells, Immobilized / Q000187:drug effects*; D002710:Chlorhexidine / Q000031:analogs & derivatives* / Q000494:pharmacology; D017097:Electric Impedance; D055664:Electrochemical Techniques / Q000379:methods*; D004566:Electrodes; D004926:Escherichia coli / Q000737:chemistry / Q000166:cytology / Q000187:drug effects*; D006046:Gold / Q000737:chemistry; D018625:Microscopy, Atomic Force; D008856:Microscopy, Fluorescence
D006046:Gold; C010882:chlorhexidine gluconate; D002710:Chlorhexidine