Study on mechanisms of colonization of nitrogen-fixing PGPB, Klebsiella pneumoniae NG14 on the root surface of rice and the formation of biofilm.
Plant growth-promoting bacteria (PGPB) refer to the bacteria beneficial to plants, and they may affect the growth and development of plants directly or indirectly. This article studied the activities of nitrogen fixation and colonization of a strain of PGPB, Klebsiella pneumoniae NG14, which was isolated from the rice root surface. The results showed that NG14 harbouring the nifH gene had nitrogenase activity, (15)N(2)-fixing activity, and was able to colonize on the root surface and within the cavity of root vascular tissues of rice. Using proteomics technology to study the differences and changes of membrane proteins (MP) of NG14 bacterial biofilm in non-biological surface, 28 proteins showing significant differences before and after the formation of bacterial biofilm have been identified, in which the precursors of membrane pore protein OmpC relevant to osmotic stress resistance was up-regulated. This study would have positive significance on further understanding of the direct and indirect promotion effects of PGPB and related mechanisms.
College of Life Sciences, Capital Normal University, Beijing 100048, People's Republic of China.
Yang
Y
Liu
Hui
H
Wang
Xiaolu
X
Sun
Hailian
H
Yang
Yunshan
Y
Wang
Wei
W
Song
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D018441:Biofilms; D015964:Gene Expression Regulation, Bacterial; D007711:Klebsiella pneumoniae / Q000145:classification / Q000235:genetics / Q000254:growth & development* / Q000502:physiology; D008969:Molecular Sequence Data; D009586:Nitrogen Fixation; D012275:Oryza / Q000382:microbiology*; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology*; D012988:Soil Microbiology
D001426:Bacterial Proteins