A universal protocol for photochemical covalent immobilization of intact carbohydrates for the preparation of carbohydrate microarrays.
A universal photochemical method has been established for the immobilization of intact carbohydrates and their analogues, and for the fabrication of carbohydrate microarrays. The method features the use of perfluorophenyl azide (PFPA)-modified substrates and the photochemical reaction of surface azido groups with printed carbohydrates. Various aldoses, ketoses, nonreducing sugars such as alditols, and their derivatives can be directly arrayed on the PFPA-modified chips. The lectin-recognition ability of arrayed mannose, glucose, and their oligo- and polysaccharides were confirmed using surface plasmon resonance imaging and laser-induced fluorescence imaging.
Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
Huibin
H
Wang
Yiming
Y
Zhang
Xun
X
Yuan
Yi
Y
Chen
Mingdi
M
Yan
D001386:Azides / Q000737:chemistry; D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000737:chemistry*; D006845:Hydrocarbons, Fluorinated / Q000737:chemistry; D037102:Lectins / Q000378:metabolism; D008027:Light; D046228:Microarray Analysis / Q000379:methods*; D055668:Photochemical Processes; D013499:Surface Properties
D001386:Azides; D002241:Carbohydrates; D006845:Hydrocarbons, Fluorinated; D037102:Lectins; C556136:perfluorophenyl azide