Detection of type III secretion system virulence and mutations in gyrA and parC genes among quinolone-resistant strains of Pseudomonas aeruginosa isolated from imported shrimp.
Twenty Pseudomonas aeruginosa isolates were recovered from imported frozen raw shrimp sold in the United States. Isolates were tested for antimicrobial susceptibility to quinolones and analyzed for mutations in quinolone resistance-determining regions, presence of type III secretion system genes, and genetic relatedness using pulsed-field gel electrophoresis. All isolates were resistant to nalidixic acid. Polymerase chain reaction assays detected exoS, exoT, exoU, and exoY among isolates. Eight unique pulsed-field gel electrophoresis clusters were generated. Mutations were found in gyrA at codon 83 (Ile to Thr) and in parC at codon 87 (Leu to Ser). Together, these findings reveal that imported shrimp may harbor virulent and quinolone-resistant strains of P. aeruginosa.
Division of Microbiology, National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, Arkansas 72079, USA. qttran@mdanderson.org
Quynh T
QT
Tran
Mohamed S
MS
Nawaz
Joanna
J
Deck
Steven
S
Foley
Kiet
K
Nguyen
Carl E
CE
Cerniglia
D019943:Amino Acid Substitution; D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D058947:Bacterial Secretion Systems / Q000235:genetics; D027081:DNA Gyrase / Q000235:genetics*; D027101:DNA Topoisomerase IV / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D024881:Drug Resistance, Bacterial / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism; D008826:Microbial Sensitivity Tests; D009154:Mutation; D009268:Nalidixic Acid / Q000494:pharmacology; D033561:Penaeidae / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D015363:Quinolones / Q000494:pharmacology; D014481:United States; D014774:Virulence
D000890:Anti-Infective Agents; D058947:Bacterial Secretion Systems; D004269:DNA, Bacterial; D026901:Membrane Transport Proteins; D015363:Quinolones; D009268:Nalidixic Acid; D027101:DNA Topoisomerase IV; D027081:DNA Gyrase