Synthesis of silica-coated AgI nanoparticles and immobilization of proteins on them.
This paper describes a method for preparing silica-coated silver iodide (AgI/SiO2) particles and immobilizing proteins on the AgI/SiO2 particles. Colloid solution of AgI particles was prepared by mixing AgClO4 aqueous solution and KI aqueous solution. Silica-coating of the AgI particles was performed by adding 3-mercaptopropyltrimethoxysilane, tetraethylorthosilicate/ethanol solution and NaOH aqueous solution successively to the AgI colloid solution. TEM observation revealed that the AgI nanoparticles were coated with uniform silica shell. The AgI/SiO2 particles were surface-modified with 3-aminopropyltrimethoxysilane and succinic anhydride. It was confirmed by XPS measurement that amino group or carboxyl group was introduced onto the AgI/SiO2 particles. Protein-immobilization was performed in aqueous solution with bovine serum albumin (BSA) in the presence of the surface-modified AgI/SiO2 particles. UV-VIS absorption spectroscopy revealed that the BSA was adsorbed on the surface-modified particles.
College of Engineering, Ibaraki University, Hitachi, Ibaraki 316-8511, Japan.
Yoshio
Y
Kobayashi
Marie
M
Minato
Kazuaki
K
Ihara
Masahide
M
Sato
Noboru
N
Suzuki
Motohiro
M
Takeda
Noriaki
N
Ohuchi
Atsuo
A
Kasuya
D006046:Gold / Q000737:chemistry*; D007454:Iodides / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles; D011506:Proteins / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
D007454:Iodides; D011506:Proteins; D006046:Gold; D012822:Silicon Dioxide