Matrix metalloproteinase 9 inhibition reduces early brain injury in cortex after subarachnoid hemorrhage.
This study investigated the role of matrix metalloproteinase-9 (MMP-9) in early brain injury (EBI) after subarachnoid hemorrhage (SAH). Sprague-Dawley male rats (n=30) between 250 and 300 g were used. SAH was produced by injecting autologous arterial blood into the prechiasmatic cistern. SB-3CT, a selective MMP-9 inhibitor, was injected intraperitoneally after SAH induction. MMP-9 protein expression was measured by western blot; laminin expression and neuronal cells in the cerebral cortex were studied by immunohistochemistry and TUNEL staining at 24h after SAH. MMP-9 expression was increased after SAH and decreased by SB-3CT inhibition at 24h after SAH (P<0.01). Laminin, the substrate of MMP-9, was decreased at 24h after SAH, and SB-3CT prevented laminin degradation. The number of TUNEL-positive neurons in cerebral cortex was increased after SAH and decreased by SB-3CT (P<0.01). MMP-9 may be involved in EBI after SAH and inhibition of MMP-9 may reduce EBI in cerebral cortex.
Department of Neurosurgery, First Hospital of Chongqing Medical University, Chongqing, 400016, People's Republic of China.
Zong-duo
ZD
Guo
Xiao-dong
XD
Zhang
Hai-tao
HT
Wu
Bin
B
Lin
Xiao-chuan
XC
Sun
John H
JH
Zhang
D000704:Analysis of Variance; D000818:Animals; D001930:Brain Injuries / Q000201:enzymology / Q000209:etiology / Q000473:pathology; D002540:Cerebral Cortex / Q000187:drug effects / Q000201:enzymology* / Q000473:pathology; D004195:Disease Models, Animal; D015536:Down-Regulation / Q000187:drug effects / Q000502:physiology*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006573:Heterocyclic Compounds, 1-Ring / Q000494:pharmacology; D020287:In Situ Nick-End Labeling; D007797:Laminin / Q000378:metabolism; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D009474:Neurons; D051381:Rats; D017207:Rats, Sprague-Dawley; D013345:Subarachnoid Hemorrhage / Q000150:complications*; D013450:Sulfones / Q000494:pharmacology
D004791:Enzyme Inhibitors; D006573:Heterocyclic Compounds, 1-Ring; D007797:Laminin; C429533:SB 3CT compound; D013450:Sulfones; D020780:Matrix Metalloproteinase 9