A method of HIV-1 inactivation compatible with antibody-based depletion of abundant proteins from plasma.
We have examined several methods, including heat treatment and treatment with detergents, to inactivate HIV-1 present in plasma to be depleted of abundant proteins utilizing an antibody-based technology. Treatment with Triton X-100 was not compatible with abundant protein depletion with an antibody column and heat treatment alters the composition of the plasma proteome. However, treatment with 1.2% N-octylglucoside for 5 min completely inhibited HIV-1 infectivity. The detergent was easily removed through buffer exchange, and this treatment had no discernable effect on protein depletion.
Laboratory of Molecular Cell Biology, SAIC-Frederick, National Cancer Institute-Frederick, Frederick, MD, USA.
Allen
A
Bosley
Heather N
HN
Marshall
Yunden
Y
Badralmaa
Ven
V
Natarajan