Biotinylated lipid membrane patterns supported by proteins for the recognition of streptavidined polystyrene microspheres.
Human serum albumin (HSA) patterns constructed on glass substrate by the micro-contact printing (microCP) technique are fabricated to support phospholipids membranes functionalized with biotinylated lipid. The experiment results indicate that this kind of lipid membrane has been assembled on HSA patterns with good stability. And the streptavidin-coated PS microspheres can be deposited on such lipid patterns with high efficiency. In this way, it may create a route to change the biological interface in the fabrication of biosensor.
Beijing National Lab for Molecular Sciences, Key Lab of Colloid and Interface Science, The Center for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
Deyao
D
Hua
Yang
Y
Yang
Xiaoming
X
Zhang
Pengli
P
Zhu
Jinbo
J
Fei
Junbai
J
Li
D001710:Biotin / Q000737:chemistry* / Q000378:metabolism; D005898:Glass; D006801:Humans; D008081:Liposomes / Q000737:chemistry* / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D055616:Microtechnology / Q000379:methods; D010316:Particle Size; D010743:Phospholipids / Q000737:chemistry* / Q000378:metabolism; D011137:Polystyrenes / Q000737:chemistry*; D012709:Serum Albumin / Q000737:chemistry / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D019809:Streptavidin / Q000737:chemistry* / Q000378:metabolism
D008081:Liposomes; D010743:Phospholipids; D011137:Polystyrenes; D012709:Serum Albumin; D001710:Biotin; D019809:Streptavidin