Ischemic preconditioning and remote ischemic preconditioning have protective effect against cold ischemia-reperfusion injury of rat small intestine.
OBJECTIVE
To investigate the protective effect of ischemic preconditioning (IPC) and remote ischemic preconditioning (RIPC) against cold ischemia-reperfusion injury (IRI) associated with small bowel transplantation (SBT).


METHODS
Male Lewis rats weighing 200-300 g were used for this study. The rats were assigned to three groups: control, ischemic preconditioning (IPC), or remote ischemic preconditioning (RIPC). Heterotopic SBT was thereafter performed. The recipient rats were killed 3, 6, 12 and 24 h after transplantation. Specimens from the intestine were histologically scored according to a grading system (Park et al.). Serum lactate dehydrogenase (LDH), aspirate aminotransferase (AST), alanine aminotransferase (ALT) were examined and heme oxygenase-1 (HO-1) were analyzed by ELISA where HO-1 served as an indicator of protection against IRI.


RESULTS
The values of tissue injury were significantly lower in the IPC and RIPC groups than in control group at 3 h after SBT. The serum LDH, AST and ALT levels also significantly decreased in the IPC and RIPC groups at 3 h after SBT, but these protective effects against cold IRI diminished by 12 and 24 h after SBT. The serum HO-1 level significantly increased in the IPC and RIPC groups 3 h after SBT.


CONCLUSIONS
Both IPC and RIPC were found to ameliorate ischemia-reperfusion injury after rat SBT in the early phase. HO-1 may therefore play a protective role against cold IRI.
Kyushu University, Fukuoka, Japan. isamu@pedsurg.med.kyushu-u.ac.jp
Isamu
I
Saeki
Toshiharu
T
Matsuura
Makoto
M
Hayashida
Tomoaki
T
Taguchi
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D015415:Biomarkers / Q000097:blood; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D051547:Heme Oxygenase-1 / Q000097:blood; D007036:Hypothermia, Induced / Q000379:methods*; D007421:Intestine, Small / Q000637:transplantation*; D019194:Ischemic Preconditioning / Q000379:methods*; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008297:Male; D051381:Rats; D011917:Rats, Inbred Lew; D015427:Reperfusion Injury / Q000201:enzymology / Q000473:pathology / Q000517:prevention & control*; D016896:Treatment Outcome
D015415:Biomarkers; D007770:L-Lactate Dehydrogenase; D051547:Heme Oxygenase-1; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase