Study on fluorescence and DNA-binding of praseodymium(III) complex containing 2,2'-bipyridine.
The fluorescence of praseodymium(III) complex containing 2,2'-bipyridine, [Pr(bpy)2Cl3·OH2] has been investigated in details. Also, the biological activity of [Pr(bpy)2Cl3·OH2] has been evaluated by examining its ability to bind to DNA with UV-vis, fluorescence as well as viscosity measurement. The fluorescence of [Pr(bpy)2Cl3·OH2] is strongly quenched through static mechanism in the presence of DNA. DNA intrinsic binding constant, Kb the binding site number, n the Stern-Volmer quenching constant, KSV and the thermodynamic parameters have been determined by fluorescence spectroscopy. For characterization of bonding mode, the effect of various experimental parameters were investigated on the interaction of [Pr(bpy)2Cl3·OH2] with DNA.
Department of Chemistry, University of Sistan & Baluchestan, P.O. Box 98155-147, Zahedan, Iran. mkhorasani@chem.usb.ac.ir
Mozhgan
M
Khorasani-Motlagh
Meissam
M
Noroozifar
Sommaieh
S
Khmmarnia
D015082:2,2'-Dipyridyl / Q000378:metabolism*; D000042:Absorption; D000818:Animals; D004247:DNA / Q000378:metabolism*; D005399:Fishes; D011193:Potassium Iodide / Q000737:chemistry; D011221:Praseodymium / Q000378:metabolism*; D012965:Sodium Chloride / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013816:Thermodynamics; D014783:Viscosity
D011193:Potassium Iodide; D012965:Sodium Chloride; D015082:2,2'-Dipyridyl; D004247:DNA; D011221:Praseodymium