[Anti-inflammatory and repair activity of two toothpastes in an ex-vivo human gingival mucosa model].
BACKGROUND
Our aim was to compare the anti-inflammatory activity of two toothpastes, one including enoxolone 1%, the other including plant extracts and sodium bicarbonate.


METHODS
Gingival fragments were kept alive ex-vivo. Inflammation, inflammatory mediators (SP and LPS) were applied to culture medium on contact with corium to induce inflammation. The effect of both toothpastes was assessed with histological and biochemical parameters (inflammatory cytokine IL8) of inflammation on the synthesis of collagen and cellular viability.


RESULTS
Both toothpaste "A" including enoxolone at 1% and "P" including plant extracts and sodium bicarbonate were effective on edema and vasodilatation. "A" acted on IL8 synthesis, unlike "P". Both toothpastes boosted collagen synthesis by fibroblasts. The percentage of cellular viability for "A" was superior to the currently admitted standard (80%), unlike to "P".


CONCLUSIONS
The mechanisms of action of each toothpaste seem to be different. "A" modulates pro-inflammatory cytokine IL8 expression, unlike "P". The toothpaste "A" seems to be better tolerated.
Service de stomatologie et de chirurgie maxillofaciale, hôpital de la Pitié-Salpêtrière, 47-83, boulevard de l'Hôpital, 75013 Paris, France.
S
S
Boisnic
L
L
Ben Slama
M-C
MC
Branchet-Gumila
D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002196:Capillaries / Q000187:drug effects; D018929:Cell Culture Techniques; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000187:drug effects; D003124:Colorimetry; D004487:Edema / Q000473:pathology; D005881:Gingiva / Q000098:blood supply / Q000166:cytology / Q000187:drug effects*; D006034:Glycyrrhetinic Acid / Q000494:pharmacology; D006801:Humans; D018836:Inflammation Mediators / Q000494:pharmacology; D016209:Interleukin-8 / Q000032:analysis / Q000187:drug effects; D008070:Lipopolysaccharides / Q000494:pharmacology; D010936:Plant Extracts / Q000494:pharmacology; D017693:Sodium Bicarbonate / Q000494:pharmacology; D013053:Spectrophotometry; D013373:Substance P / Q000494:pharmacology; D014100:Toothpastes / Q000494:pharmacology*; D014945:Wound Healing / Q000187:drug effects
D000893:Anti-Inflammatory Agents; D018836:Inflammation Mediators; D016209:Interleukin-8; D008070:Lipopolysaccharides; C091016:Parodontax; D010936:Plant Extracts; D014100:Toothpastes; D013373:Substance P; D017693:Sodium Bicarbonate; D003094:Collagen; D006034:Glycyrrhetinic Acid