The grapevine-shoot extract Vineatrol30 inhibits the chemically induced malignant transformation of BALB/c-3T3 cells.
Vineatrol®30 (developed and produced jointly by Breko GmbH [Bremen, Germany] and Actichem [Montauban, France]) is a grapevine-shoot extract that contains resveratrol as well as considerable amounts of resveratrol oligomers. In the present study it is shown that Vineatrol30 at a noncytotoxic concentration of 2.3 μg/mL significantly reduced the number of malignantly transformed foci induced by a sequential treatment of BALB/c-3T3 cells with 3-methylcholanthrene and 12-O-tetradecanoylphorbol 13-acetate in the so-called BALB/c-3T3 cell transformation assay. At a higher concentration Vineatrol30 drastically decreased the relative plating efficiency of the cells. Furthermore, the results suggest that the resveratrol oligomers present in Vineatrol30, independently from resveratrol itself, were indeed able to inhibit the formation of malignantly transformed BALB/c-3T3 foci.
Chair of Nutritional Toxicology, Institute of Nutritional Science, University of Potsdam, Nuthetal, Germany.
Carsten
C
Müller
Kristina
K
Ullmann
Pablo
P
Steinberg
D016475:3T3 Cells; D000818:Animals; D002273:Carcinogens / Q000633:toxicity; D002471:Cell Transformation, Neoplastic / Q000139:chemically induced / Q000187:drug effects*; D015536:Down-Regulation / Q000187:drug effects*; D008748:Methylcholanthrene / Q000633:toxicity; D051379:Mice; D008807:Mice, Inbred BALB C; D010703:Phorbol Esters / Q000633:toxicity; D010936:Plant Extracts / Q000494:pharmacology*; D018520:Plant Shoots / Q000737:chemistry; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*; D027843:Vitis / Q000737:chemistry*
D002273:Carcinogens; D010703:Phorbol Esters; D010936:Plant Extracts; D013267:Stilbenes; D008748:Methylcholanthrene; C032974:12-O-retinoylphorbol-13-acetate; D000077185:Resveratrol