Quantitative proteomic approaches for biomarker discovery.
The rapid advances in proteomic technologies have made possible systematic analysis of hundreds to thousands of proteins in clinical samples with the promise of uncovering novel protein biomarkers for various disease conditions. We will discuss in this review article current MS and protein chip-based quantitative proteomic approaches and their application in biomarker discovery. The emphasis will be placed on new quantification strategies employing stable isotopic labeling coupled with MS/MS, and antibody-based protein chips and nanodevices. The strength and weakness of each technology are briefly highlighted.
Institute for Systems Biology, Seattle, WA, USA.
Zhiyuan
Z
Hu
Leroy
L
Hood
Qiang
Q
Tian