Isoflurane pre-treatment before cardiopulmonary bypass alleviates neutrophil accumulation in dog lungs.
OBJECTIVE
This study investigated the effect of isoflurane pretreatment on cardiopulmonary bypass (CPB)-related lung injury.


METHODS
Twelve dogs were randomly divided into two groups of six each. In one group, 1.0 minimum alveolar concentration (MAC) of isoflurane was dministered for 30 min before CPB, while the control group received no anaesthetic. Both groups then underwent 100 min of mild hypothermic CPB with 60-min aortic cross clamping. Haemodynamic parameters, respiratory mechanics and alveolar arterial oxygen difference (AaDO₂) were measured during the experiment. One hundred and fifty minutes after CPB, lung tissue samples from the non-dependent and dependent portions of the left and right lungs were harvested for polymorphonulear leukocyte (PMNs) counts.


RESULTS
Following CPB, within the control group, pulmonary vascular resistance (PVR) was significantly increased at 60, 120 and 180 min after declamping, AaDO₂ deteriorated at 180 min post-declamping, and dynamic lung compliance (DLC) was reduced dramatically after declamping. Isoflurane pretreatment before CPB significantly reduced PVR compared to the controls. AaDO₂ was impaired at 180 min after declamping and DLC was decreased after declamping within the isoflurane group. No differences in AaDO₂ and DLC were found between the isoflurane and control groups. At 180 min after declamping, the PMN count in both the non-dependent and dependent regions of the isoflurane pre-treated lungs was significantly lower than that of the controls.


CONCLUSIONS
Our results suggest that 30-min pre-treatment with 1.0 MAC isoflurane before CPB caused a reduction in PMN accumulation in the dog lungs, inhibition of increases in PVR, and it did not affect AaDO₂ in the early post-CPB stage.
Department of Anesthesiology, West China Hospital, Sichuan University, Chengdu 610041, Sichuan, China.
Gui-Zhi
GZ
Du
Hong
H
Gao
Jin
J
Liu
Guan-Sheng
GS
Wu
Xiang
X
He
Xiang-Gang
XG
Zeng
Xaun-Yi
XY
Hu
Xin-Hua
XH
Li
D055371:Acute Lung Injury / Q000209:etiology / Q000503:physiopathology / Q000517:prevention & control*; D018685:Anesthetics, Inhalation / Q000627:therapeutic use*; D000818:Animals; D002315:Cardiopulmonary Bypass / Q000009:adverse effects*; D004285:Dogs; D006439:Hemodynamics; D007530:Isoflurane / Q000627:therapeutic use*; D007958:Leukocyte Count; D009504:Neutrophils / Q000187:drug effects*; D015656:Respiratory Mechanics
D018685:Anesthetics, Inhalation; D007530:Isoflurane