Use of DT40 conditional-knockout cell lines to study chromosomal passenger protein function.
The CPC [chromosomal passenger complex; INCENP (inner centromere protein), Aurora B kinase, survivin and borealin] is implicated in many mitotic processes. In the present paper we describe how we generated DT40 conditional-knockout cell lines for incenp1 and survivin1 to better understand the role of these CPC subunits in the control of Aurora B kinase activity. These lines enabled us to reassess current knowledge of survivin function and to show that INCENP acts as a rheostat for Aurora B activity.
Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh EH9 3JR, Scotland, UK.
Xavier
X
Fant
Kumiko
K
Samejima
Ana
A
Carvalho
Hiromi
H
Ogawa
Zhenjie
Z
Xu
Zuojun
Z
Yue
William C
WC
Earnshaw
Sandrine
S
Ruchaud
D000818:Animals; D017209:Apoptosis; D064107:Aurora Kinase B; D064127:Aurora Kinases; D002460:Cell Line; D002645:Chickens; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics / Q000378:metabolism*; D002875:Chromosomes / Q000378:metabolism; D055785:Gene Knockdown Techniques; D006801:Humans; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism*; D008938:Mitosis; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*
D002868:Chromosomal Proteins, Non-Histone; D008869:Microtubule-Associated Proteins; C576921:AURKB protein, human; D064107:Aurora Kinase B; D064127:Aurora Kinases; D017346:Protein Serine-Threonine Kinases