Enhanced production of human serum albumin by fed-batch culture of Hansenula polymorpha with high-purity oxygen.
Fed-batch cultures of Hansenula polymorpha were studied to develop an efficient biosystem to produce recombinant human serum albumin (HSA). To comply with this purpose, we used high purity oxygen supplying strategy to increase viable cell density in a bioreactor and enhance the production of target protein. A mutant strain, H. polymorpha GOT7 was utilized in this study as a host strain in both 5-L and 30-L scale fermentors. To supply high purity oxygen into a bioreactor, nearly 100 % high purity oxygen from commercial bomb or higher than 93 % oxygen available in-situ from a pressure swing adsorption oxygen generator (PSA) was employed. Under the optimal fermentation of H. polymorpha with high purity oxygen, the final cell densities and produced HSA concentrations were 24.6 g/L and 5.1 g/L in the 5-L fermentor, and 24.8 g/L and 4.5 g/L in the 30-L fermentor, respectively. These were about 2-10 times higher than those obtained in air-based fed-batch fermentations. The discrepancies between the 5-L and 30-L fermentors with air supply were presumably due to the higher contribution of surface aeration over submerged aeration in the 5-L fermentor. This study, therefore, proved the positive effect of high purity oxygen to enhance viable cell density as well as target recombinant protein production in microbial fermentations.
Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Korea.
Jong Kyu
JK
Youn
Longan
L
Shang
Moon Il
MI
Kim
Chang Moon
CM
Jeong
Ho Nam
HN
Chang
Moon Sun
MS
Hahm
Sang Ki
SK
Rhee
Hyun Ah
HA
Kang
D019149:Bioreactors / Q000382:microbiology; D005285:Fermentation; D006801:Humans; D010100:Oxygen / Q000378:metabolism*; D010843:Pichia / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D012709:Serum Albumin / Q000096:biosynthesis* / Q000235:genetics
D011994:Recombinant Proteins; D012709:Serum Albumin; D010100:Oxygen