Isolation and characterization of a novel myoactive tetradecapeptide-related peptide isolated from the brain of the squid, Todarodes pacificus.
A new bioactive tetradecapeptide, GFKDNVSNRIAHGFamide, was isolated from the brain of the squid, Todarodes pacificus. Using isolated T. pacificus esophagus as a bioassay, the peptide was shown to induce potent contraction of smooth muscle. The threshold concentration for contraction was 5×10(-10) M to 1×10(-9) M. The peptide was homologous to other molluskan (class Gastropoda) and annelid myoactive tetradecapeptides and to some extent, to arthropodan tridecapeptides. A full-length cDNA encoding the biosynthetic precursor of the active peptide was cloned, revealing that the peptide is probably secreted following processing of a prepropeptide containing a signal peptide and prosequences. This is the first myoactive tetradecapeptide (MATP) to be isolated from any mollusk of the class Cephalopoda and we have named it Todarodes tetradecapeptide (TTP).
Department of Biotechnology, College of Fisheries Science, Pukyong National University, 599-1, Daeyeon 3-Dong, Nam-Gu, Busan 608-737, Republic of Korea.
Hye-Jin
HJ
Go
Eun Hee
EH
Jo
Jung-Kil
JK
Seo
Yong-Ki
YK
Hong
Hyung Ho
HH
Lee
Gun Do
GD
Kim
Tae Wook
TW
Park
Edward J
EJ
Noga
Nam Gyu
NG
Park
D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000378:metabolism*; D003001:Cloning, Molecular; D018076:DNA, Complementary; D049832:Decapodiformes / Q000378:metabolism*; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*
D018076:DNA, Complementary; D010455:Peptides