Manual and automated reticulocyte counts.
Manual reticulocyte counts were examined under light microscopy, using the property whereby supravital stain precipitates residual ribosomal RNA versus the automated flow methods, with the suggestion that in the latter there is greater precision and an ability to determine both mature and immature reticulocyte fractions. Three hundred and forty-one venous blood samples of patients were analyzed of whom 224 newborn and the rest adults; 51 males and 66 females, with ages between 0 and 89 years, as part of the laboratory routine for hematological examinations at the Clinical Laboratory of the Hospital Universitário do Oeste do Paraná. This work aimed to compare manual and automated methodologies for reticulocyte countings and evaluate random and systematic errors. The results obtained showed that the difference between the two methods was very small, with an estimated 0·4% systematic error and 3·9% random error. Thus, it has been confirmed that both methods, when well conducted, can reflect precisely the reticulocyte counts for adequate clinical use.
Programa de Pós-Graduação em Ciências Farmacêuticas da Universidade Federal do Paraná, Curitiba, PR, Brazil.
Mackelly
M
Simionatto
Josiane Padilha
JP
de Paula
Michele Ana Flores
MA
Chaves
Márcia
M
Bortoloso
Domenic
D
Cicchetti
Maria Suely Soares
MS
Leonart
Aguinaldo José
AJ
do Nascimento
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001331:Automation; D002648:Child; D002675:Child, Preschool; D005260:Female; D005434:Flow Cytometry / Q000379:methods* / Q000592:standards; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008853:Microscopy / Q000379:methods* / Q000592:standards; D008875:Middle Aged; D012335:RNA, Ribosomal; D015203:Reproducibility of Results; D017701:Reticulocyte Count / Q000295:instrumentation / Q000379:methods* / Q000592:standards*; D055815:Young Adult
D012335:RNA, Ribosomal