Establishment of Hertwig's epithelial root sheath cell line from cells involved in epithelial-mesenchymal transition.
The epithelial-mesenchymal transition (EMT) is an important event in the developmental process of various organs. In periodontal development during root formation of a tooth, this EMT has been a subject of controversy. Hertwig's epithelial root sheath (HERS), consisting of two epithelial layers, plays a role of inducing odontogenesis during root development and thereafter becomes fragmented. Some researchers have maintained that in the process of this fragmentation, some HERS cells change from epithelial to mesenchymal cells. Here, we established a HERS cell line (HERS01a) and examined its gene and protein expression. Immunohistochemical staining and real-time PCR analysis showed that HERS01a cells expressed vimentin and N-cadherin as mesenchymal markers as well as cytokeratin14, E-cadherin, and p63 as epithelial stem cell markers. In the presence of TGF-β, HERS01a cells also expressed many more mesenchymal markers, as well as snail1 and 2 as EMT markers. Taken together, our data show that HERS01a displayed unique features associated with EMT in the root formation process, and will thus be useful for analyzing the biological characteristics of HERS and the molecular mechanism underlying the EMT.
Division of Periodontology, Department of Conservative Dentistry and Rehabilitation, School of Dentistry, Iwate Medical University, Morioka, Iwate, Japan.
Tadashi
T
Akimoto
Naoki
N
Fujiwara
Tadayoshi
T
Kagiya
Keishi
K
Otsu
Kiyoto
K
Ishizeki
Hidemitsu
H
Harada
D000818:Animals; D002460:Cell Line; D002469:Cell Separation; D004847:Epithelial Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D058750:Epithelial-Mesenchymal Transition; D015870:Gene Expression; D007150:Immunohistochemistry; D051379:Mice; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D014092:Tooth Root / Q000166:cytology* / Q000254:growth & development / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000494:pharmacology
D012333:RNA, Messenger; D016212:Transforming Growth Factor beta