Cloning, purification and preliminary crystallographic analysis of cobalamin methyltransferases from Rhodobacter capsulatus.
Of the 30 biosynthetic steps necessary for the production of cobalamin (vitamin B12), eight involve the addition of S-adenosylmethionine-derived methyl groups to the tetrapyrrole framework. These eight methyl additions are catalysed by six canonical methyltransferase domains and one noncanonical methyltransferase domain. Recombinant forms of four methyltransferases from Rhodobacter capsulatus, CobJ, CobM, CobF and CobL, and of the C-terminal noncanonical domain of CobL (CobL-C) have been crystallized, some in more than one crystal form. Most of the crystals diffracted to beyond 2.5 Å resolution and all are suitable for structure determination. Crystals of CobM and CobJ, which are involved in ring contraction, and of CobL, which is involved in two methylations and decarboxylation, are reported for the first time.
School of Biological and Chemical Sciences, Queen Mary University of London, Mile End Road, London E1 4NS, England.
Arefeh
A
Seyedarabi
Thomas
T
Hutchison
Teng Teng
TT
To
Evelyne
E
Deery
Amanda
A
Brindley
Martin J
MJ
Warren
Richard W
RW
Pickersgill
D003001:Cloning, Molecular; D018360:Crystallography, X-Ray; D004591:Electrophoresis, Polyacrylamide Gel; D008780:Methyltransferases / Q000737:chemistry* / Q000302:isolation & purification*; D016354:Rhodobacter capsulatus / Q000201:enzymology*; D014805:Vitamin B 12 / Q000096:biosynthesis / Q000378:metabolism*
D008780:Methyltransferases; D014805:Vitamin B 12