Polyaniline-carboxymethyl cellulose nanocomposite for cholesterol detection.
Cholesterol oxidase (ChOx) has been covalently immobilized onto polyaniline-carboxymethyl cellulose (PANI-CMC) nanocomposite film deposited onto indium-tin-oxide (ITO) coated glass plate using glutaraldehyde as a cross-linker. Fourier transform infrared (FTIR) spectroscopic and electrochemical studies have been used to characterize the PANI-CMC/ITO nanocomposite electrode and ChOx/PANI-CMC/ITO bioelectrode. Scanning electron microscopy (SEM) studies reveal the formation of PANI-CMC nanocomposite fibers of size approximately 150 nm in diameter. The ChOx/PANI-CMC/ITO bioelectrode exhibits linearity as 0.5-22 mM, detection limit as 1.31 mM, sensitivity as 0.14 mA/mM cm2, response time as 10 s and shelf-life of about 10 weeks when bioelectrode is stored at 4 degrees C. The low value of Michaelis-Menten constant (K(m)) obtained as 2.71 mM reveals high affinity of immobilized ChOx for PANI-CMC/ITO nanocomposite electrode.
Department of Science and Technology Centre on Bimolecular Electronics, National Physical Laboratory, Dr. K. S. Krishnan Marg, New Delhi 110012, India.
Abdul
A
Barik
Pratima R
PR
Solanki
Ajeet
A
Kaushik
Azahar
A
Ali
M K
MK
Pandey
C G
CG
Kim
B D
BD
Malhotra
D000814:Aniline Compounds / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D002266:Carboxymethylcellulose Sodium / Q000737:chemistry*; D002784:Cholesterol / Q000032:analysis* / Q000378:metabolism; D002789:Cholesterol Oxidase / Q000737:chemistry / Q000378:metabolism; D058266:Dielectric Spectroscopy; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D016014:Linear Models; D008855:Microscopy, Electron, Scanning; D053761:Nanocomposites / Q000737:chemistry*; D010316:Particle Size; D058105:Polymerization; D012680:Sensitivity and Specificity; D017550:Spectroscopy, Fourier Transform Infrared; D017971:Tin Compounds / Q000737:chemistry
D000814:Aniline Compounds; D004800:Enzymes, Immobilized; D017971:Tin Compounds; C416807:polyaniline; C109984:indium tin oxide; D002784:Cholesterol; D002789:Cholesterol Oxidase; D002266:Carboxymethylcellulose Sodium