[Effect of zoledronic acid on the differentiation and osteoprotegerin production of osteoblasts in rabbit].
OBJECTIVE
To investigate the effect of zoledronic acid on proliferation and osteogenic differentiation of osteoblasts.


METHODS
Osteoblasts were obtained from newly born rabbit jaw bones and cultured by the method of bone-tissue cultivation. Primary cultivated osteoblast was identified by alkaline phosphatase (ALP) and the mineralization nodes. Zoledronic acid at various concentrations was added to six groups of media with serial subcultivated cells (the final concentration: 0, 10(-5), 10(-6), 10(-7), 10(-8) and 10(-9) mol/L). At different time, ALP, osteoprotegerin and osteocalcin were observed and calculated.


RESULTS
The concentration of 10(-6), 10(-7), 10(-8) and 10(-9) mol/L zoledronic acid significantly increased ALP activity [(5.91 ± 0.35), (7.62 ± 0.33), (10.00 ± 0.38), (8.91 ± 0.29) U/L]. Protein expression of osteoprotegerin and osteocalcin was enhanced. The differences among the groups were significant (P < 0.01). Peak level was attained at a concentration of 10(-8) mol/L.


CONCLUSIONS
Zoledronic acid promotes osteoblast proliferation and maturation and modulates osteoprotegerin production.
Department of Oral and Maxillofacial Surgery, Hospital of Stomatology, Tianjin Medical University, Tianjin 300070, China. zj301doctor@126.com
Jian
J
Zhang
Wen-yi
WY
Zhang
Hua
H
Sun
D000469:Alkaline Phosphatase; D000818:Animals; D001842:Bone and Bones; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D004164:Diphosphonates / Q000494:pharmacology*; D007093:Imidazoles / Q000494:pharmacology*; D010006:Osteoblasts / Q000187:drug effects*; D015675:Osteocalcin / Q000187:drug effects / Q000378:metabolism; D010012:Osteogenesis; D053244:Osteoprotegerin / Q000187:drug effects* / Q000378:metabolism; D011817:Rabbits; D000077211:Zoledronic Acid
D004164:Diphosphonates; D007093:Imidazoles; D053244:Osteoprotegerin; D015675:Osteocalcin; D000077211:Zoledronic Acid; D000469:Alkaline Phosphatase