Stability of epidoxorubicin in solid state.
The influence of temperature and relative air humidity on the stability of epidoxorubicin hydrochloride (EP) was investigated. The degradation of the substance studied was determined: (a) in dry air at 393K, (b) at relative air humidity ~76% at 333K, 343K, 353K, 363K and 373K, (c) in the relative air humidity range 50-90% at 363K. The degradation of EP in the atmosphere of increased relative air humidity was a first-order reaction relative to substance concentration and in dry, hot air (RH 0%; 393K) is a reversible first-order reaction relative to substance concentration. The dependences lnk=f(1/T) and lnk=f(RH%) were described by the equation: lnk=(35.1±10.9)-(16,250±3823)(1/T) and lnk=(3.79±3.34) × 10(-2) (RH%)-(12.9±2.4), respectively. The kinetic and thermodynamic parameters of EP degradation were calculated. The parameters of separation were following: LiChrospher RP-18 column, 5 μm, 250 mm × 4 mm; mobile phase: the mixture of equal volume of acetonitrile and the solution containing 2.88 gl(-1) of sodium laurisulfate and 2.25 ml l(-1) of phosphoric acid (V) 85%; flow rate: 1.0 ml min (-1); UV detection - 254 nm.
University of Medical Sciences, Department of Pharmaceutical Chemistry, Faculty of Pharmacy, 6 Grunwaldzka Str., Poznań, Poland. asobczak@ump.edu.pl
Agnieszka
A
Sobczak
Anna
A
Jelińska
Monika
M
Leśniewska
Artur
A
Firlej
Irena
I
Oszczapowicz
D000465:Algorithms; D000903:Antibiotics, Antineoplastic / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D003630:Daunorubicin / Q000032:analysis; D004317:Doxorubicin / Q000032:analysis; D004355:Drug Stability; D015251:Epirubicin / Q000032:analysis*; D006358:Hot Temperature / Q000009:adverse effects; D006813:Humidity / Q000009:adverse effects; D007700:Kinetics; D011208:Powders; D013678:Technology, Pharmaceutical; D013816:Thermodynamics
D000903:Antibiotics, Antineoplastic; D011208:Powders; D015251:Epirubicin; D004317:Doxorubicin; D003630:Daunorubicin