Development of rHA1-ELISA for specific and sensitive detection of H9 subtype influenza virus.
A recombinant antigen-based single serum dilution ELISA was developed for simultaneous detection and subtyping of influenza viruses. Recombinant baculovirus encoding the hemagglutinin (HA(1) subunit) of H9N2 virus was generated. To evaluate the rHA1-ELISA, microplates were coated with purified HA1 protein and tested with reference control sera. Subsequently, 92 field sera collected from chickens suspected to be infected with H9N2 AIV were employed to test the efficacy of the rHA1-ELISA. The sera were tested simultaneously by HI and a commercial AIV ELISA kit. The rHA1-ELISA appeared to be highly specific and sensitive for direct detection of H9N2 antibodies in serum samples.
Razi Vaccine & Serum Research Institute, Karaj, Iran. shahsavandi_s@yahoo.com
Shahla
S
Shahsavandi
Ali-Hatef
AH
Salmanian
Seyed Ali
SA
Ghorashi
Shahin
S
Masoudi
Fatemeh
F
Fotouhi
Mohammad Majid
MM
Ebrahimi
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D002645:Chickens; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006389:Hemagglutinins, Viral / Q000235:genetics; D053127:Influenza A Virus, H9N2 Subtype / Q000276:immunology* / Q000302:isolation & purification*; D005585:Influenza in Birds / Q000175:diagnosis* / Q000821:virology; D011994:Recombinant Proteins / Q000235:genetics; D012680:Sensitivity and Specificity; D014773:Virology / Q000379:methods*
D000914:Antibodies, Viral; D006389:Hemagglutinins, Viral; D011994:Recombinant Proteins; C458881:hemagglutinin fusogenic peptide, influenza virus