Dialysis sampling on-line coupled with high-performance liquid chromatography for simultaneous investigation of the interactions between multi-components in herbs and the albumin.
An on-line dialysis sampling method coupled with high-performance liquid chromatography was developed for simultaneous investigation of the interactions between multiple bioactive compounds contained in herbal medicines and proteins. The system was used to estimate the interactions of components in danshen (Salvia miltiorrhiza) injection with bovine serum albumin. The results showed that the binding actions of five water-soluble compounds in danshen injection could be simultaneously investigated. The binding parameters of caffeic acid, ferulic acid, danshensu, protocatechuic acid and protocatechuic aldehyde were obtained and the interaction mechanisms were explored. The association constant evaluated for caffeic acid agreed well with literature values. The proposed approach should be beneficial for examining the holistic combined action of herbal medicine with proteins and help facilitate the discovery process of drug candidates.
Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Material Science, Shaanxi Normal University, Xi'an, China.
Yu-Ling
YL
Wang
Jiang-Feng
JF
Yuan
Wei
W
Shang
Jing
J
Zhang
Zhi-Qi
ZQ
Zhang
D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003956:Dialysis / Q000379:methods*; D007267:Injections; D009862:Online Systems; D011485:Protein Binding; D027561:Salvia miltiorrhiza / Q000737:chemistry / Q000378:metabolism*; D012710:Serum Albumin, Bovine / Q000378:metabolism*; D012995:Solubility; D013816:Thermodynamics; D013997:Time Factors; D014867:Water / Q000737:chemistry
D014867:Water; D012710:Serum Albumin, Bovine