[In vitro co-cultivation of Toxoplasma gondii tachyzoites with rat brain astrocytes].
Purified astrocytes were cultured in plates. When astrocytes grew over 80% of the plate, tachyzoites of Toxoplasma gondii RH strain were added for co-culture. In the period of 0-72 h, change of the astrocytes and tachyzoites was observed after Giemsa staining. In 0-48 h, monodansylcadaverine (MDC) was used to study the action of autophagy in the process of tachyzoites invading astrocytes. At 1 h co-culture, tachyzoites had entered in astrocytes and the autophagosomes appeared. At 4 h, the autophagosomes increased pronouncedly. However, after 12 h, number of autophagosomes considerably decreased and damage of the cells occurred. 48 h later, autophagosomes disappeared and more astrocytes were destroyed. At 72 h most cells destroyed and tachyzoites were released. The result showed that autophagy is inhibited when the astrocytes were in vitro infected by tachyzoites.
Department of Pathobiology, Soochow University, Suzhou 215123, China.
Dong-na
DN
Li
You-sheng
YS
Liang
Yong-hua
YH
Zhou
Huan-xiang
HX
Zhang
Hai-ying
HY
Sheng
Wei
W
Luo
Wei
W
Gong
Hong-xiang
HX
Zhuge
D000818:Animals; D001253:Astrocytes / Q000166:cytology* / Q000469:parasitology*; D002478:Cells, Cultured; D018920:Coculture Techniques; D051379:Mice; D008815:Mice, Inbred Strains; D051381:Rats; D017207:Rats, Sprague-Dawley; D014122:Toxoplasma / Q000254:growth & development*