Analytical method for assessing potential dermal exposure to pesticides of a non-agricultural occupationally exposed population.
To measure dermal exposure of a non-agricultural occupationally exposed population to pesticides, a new method has been developed for analysis of 13 pesticides from different classes (fungicides, herbicides, insecticides) on dermal patches. The method includes extraction of the patches and analysis of the pesticides by GC-MS and/or HPLC-fluorescence. Water-soluble pesticides (glyphosate and glufosinate) on patches were ultrasonically extracted twice with ultra-pure water for 10 min and analysed by HPLC-fluorescence after derivatisation with FMOC. Organic-soluble pesticides (bifenthrin, cyprodinil, difufenicanil, fludioxonil, oxadiazon, pyriproxyfen, clopyralid, 2,4-D, fluroxypyr, 2,4-MCPA, and triclopyr) were extracted ultrasonically twice for 10 min with 70:30 dichloromethane-acetonitrile and analysed by GC-MS directly or after derivatisation with N-methyl-N-tert-butyldimethylsilyltrifluoroacetamide. Detection limits varied between 3 and 4 μg L(-1) for water-soluble pesticides and between 1 and 10 μg L(-1) for organic-soluble pesticides.
Equipe de Physico-Chimie de l'Atmosphère, LMSPC (UMR 7515 CNRS, Université de Strasbourg), 1 rue Blessig, 67084 Strasbourg, Cedex, France.
Olivier
O
Delhomme
Caroline
C
Raeppel
Delphine
D
Teigné
Olivier
O
Briand
Maurice
M
Millet
D002851:Chromatography, High Pressure Liquid; D004781:Environmental Exposure / Q000032:analysis*; D004785:Environmental Pollutants / Q000032:analysis*; D005453:Fluorescence; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D016273:Occupational Exposure / Q000032:analysis*; D010575:Pesticides / Q000032:analysis*; D012867:Skin / Q000737:chemistry*
D004785:Environmental Pollutants; D010575:Pesticides