Matrix polyelectrolyte capsules based on polysaccharide/MnCO₃ hybrid microparticle templates.
An efficient strategy for biomacromolecule encapsulation based on spontaneous deposition into polysaccharide matrix-containing capsules is introduced in this study. First, hybrid microparticles composed of manganese carbonate and ionic polysaccharides including sodium hyaluronate (HA), sodium alginate (SA) and dextran sulfate sodium (DS) with narrow size distribution were synthesized to provide monodisperse templates. Incorporation of polysaccharide into the hybrid templates was successful as verified by thermogravimetric analysis (TGA) and confocal laser scanning microscopy (CLSM). Matrix polyelectrolyte microcapsules were fabricated through layer-by-layer (LbL) self-assembly of oppositely charged polyelectrolytes (PEs) onto the hybrid particles, followed by removal of the inorganic part of the cores, leaving polysaccharide matrix inside the capsules. The loading and release properties of the matrix microcapsules were investigated using myoglobin as a model biomacromolecule. Compared to matrix-free capsules, the matrix capsules had a much higher loading capacity up to four times; the driving force is mostly due to electrostatic interactions between myoglobin and the polysaccharide matrix. From our observations, for the same kind of polysaccharide, a higher amount of polysaccharide inside the capsules usually led to better loading capacity. The release behavior of the loaded myoglobin could be readily controlled by altering the environmental pH. These matrix microcapsules may be used as efficient delivery systems for various charged water-soluble macromolecules with applications in biomedical fields.
National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, PR China.
Qingrong
Q
Wei
Hua
H
Ai
Zhongwei
Z
Gu
D002214:Capsules / Q000737:chemistry*; D002254:Carbonates / Q000737:chemistry*; D008345:Manganese / Q000737:chemistry*; D018613:Microscopy, Confocal; D011134:Polysaccharides / Q000737:chemistry*; D013818:Thermogravimetry
D002214:Capsules; D002254:Carbonates; D011134:Polysaccharides; D008345:Manganese; C045327:manganese carbonate