Enzymatic synthesis of vancomycin derivatives using galactosyltransferase and sialyltransferase.
Analogs of vancomycin and pseudo-vancomycin with new sugar attachments in mono- and di-saccharide form have been enzymatically synthesized by glycosylation with overexpressed glycosyltransferases, β1,4-galactosyltransferase and α2,3-sialyl transferases. All four analogs, including galactose-containing derivatives (6 and 8) and galactose- and sialic acid-containing derivatives (7 and 9) were prepared and characterized by HPLC, LC-MS, NMR and MIC test.
Institute of Biomolecule Reconstruction (iBR), Department of Pharmaceutical Engineering, Sun Moon University, Kalsan-ri, Tangjeong-myeon, Asansi, Chungnam, Republic of Korea.
Tae-Jin
TJ
Oh
Dae Hee
DH
Kim
Sun Youp
SY
Kang
Tokutaro
T
Yamaguchi
Jae Kyung
JK
Sohng
D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D002853:Chromatography, Liquid; D005690:Galactose / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D005700:Galactosyltransferases / Q000378:metabolism*; D006031:Glycosylation; D016695:Glycosyltransferases / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008826:Microbial Sensitivity Tests; D019158:N-Acetylneuraminic Acid / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D014640:Vancomycin / Q000031:analogs & derivatives* / Q000096:biosynthesis* / Q000737:chemistry / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D014640:Vancomycin; D016695:Glycosyltransferases; D005700:Galactosyltransferases; D019158:N-Acetylneuraminic Acid; D005690:Galactose