A novel role of periostin in postnatal tooth formation and mineralization.
Periostin plays multiple functions during development. Our previous work showed a critical role of this disulfide-linked cell adhesion protein in maintenance of periodontium integrity in response to occlusal load. In this study, we attempted to address whether this mechanical response molecule played a direct role in postnatal tooth development. Our key findings are 1) periostin is expressed in preodontoblasts, and odontoblasts; and the periostin-null incisor displayed a massive increase in dentin formation after mastication; 2) periostin is also expressed in the ameloblast cells, and an enamel defect is identified in both the adult-null incisor and molar; 3) deletion of periostin leads to changes in expression profiles of many non-collagenous protein such as DSPP, DMP1, BSP, and OPN in incisor dentin; 4) the removal of a biting force leads to reduction of mineralization, which is partially prevented in periostin-null mice; and 6) both in vitro and in vivo data revealed a direct regulation of periostin by TGF-β1 in dentin formation. In conclusion, periostin plays a novel direct role in controlling postnatal tooth formation, which is required for the integrity of both enamel and dentin.
Department of Respiratory Medicine, Qilu Hospital, Shandong University, Jinan 250012, China.
Dedong
D
Ma
Rong
R
Zhang
Yao
Y
Sun
Hector F
HF
Rios
Naoto
N
Haruyama
Xianglong
X
Han
Ashok B
AB
Kulkarni
Chunlin
C
Qin
Jian Q
JQ
Feng
D000565:Ameloblasts / Q000378:metabolism; D000818:Animals; D002113:Calcification, Physiologic / Q000502:physiology*; D015815:Cell Adhesion Molecules / Q000096:biosynthesis* / Q000235:genetics; D003743:Dental Enamel / Q000378:metabolism; D003804:Dentin / Q000378:metabolism; D017353:Gene Deletion; D005786:Gene Expression Regulation / Q000502:physiology*; D007180:Incisor / Q000254:growth & development* / Q000378:metabolism; D008409:Mastication / Q000502:physiology; D051379:Mice; D008822:Mice, Transgenic; D008963:Molar / Q000254:growth & development* / Q000378:metabolism; D009804:Odontoblasts / Q000378:metabolism; D053773:Transforming Growth Factor beta1 / Q000235:genetics / Q000378:metabolism
D015815:Cell Adhesion Molecules; C082838:Postn protein, mouse; D053773:Transforming Growth Factor beta1