Flow-based combinatorial antibody profiling: an integrated approach to cell characterization.
BD FACS™ CAP (CAP = combinatorial antibody profile) is a screening tool for rapid characterization of human cell surface protein expression profiles using semi-automated high-throughput flow cytometry. The current configuration consists of 229 directly conjugated antibodies arrayed in a 96-well plate as three-color cocktails, which enables the characterization of each of the 229 individual surface markers. Each individual cell type of interest is analyzed on the 96-well screening plates and the data are acquired on a flow cytometer equipped with a high-throughput sampler. The expression level of each marker for each cell type is then calculated using semiautomated custom flow cytometry software. The process of characterizing these surface markers in a highly efficient manner using BD FACS™ CAP is enabled by automated liquid handling for staining, automated flow cytometry for data acquisition, and standardized algorithms for automated data analysis.
BD Biosciences, San Diego, CA, USA.
Shane
S
Bruckner
Ling
L
Wang
Ruiling
R
Yuan
Perry
P
Haaland
Amitabh
A
Gaur
D000906:Antibodies / Q000276:immunology*; D000954:Antigens, Surface / Q000235:genetics / Q000276:immunology; D002478:Cells, Cultured; D016000:Cluster Analysis; D001330:Electronic Data Processing; D005434:Flow Cytometry; D005456:Fluorescent Dyes / Q000378:metabolism; D020869:Gene Expression Profiling / Q000295:instrumentation / Q000379:methods*; D057166:High-Throughput Screening Assays; D006801:Humans; D011786:Quality Control; D012680:Sensitivity and Specificity; D012984:Software; D013194:Staining and Labeling
D000906:Antibodies; D000954:Antigens, Surface; D005456:Fluorescent Dyes