Molecular cloning and ontogenic mRNA expression of fatty acid desaturase in the carnivorous striped snakehead fish (Channa striata).
There is very little information on the capacity of freshwater carnivorous fish to biosynthesize highly unsaturated fatty acids (HUFA). The striped snakehead fish (Channa striata) is a carnivorous species cultured inland of several Southeast Asian countries due to its pharmaceutical properties in wound healing enhancement. We described here the full-length cDNA cloning of a striped snakehead fatty acid desaturase (fads), which is responsible for desaturation of unsaturated fatty acids in the HUFA biosynthesis. Bioinformatics analysis reveals a protein coding region with length of 445 amino acids containing all characteristic features of desaturase enzyme, including a cytochrome b5-domain with the heme-binding motif, two transmembrane domains and three histidine-rich regions. The striped snakehead fads amino acid sequence shares high similarity with known fads of other teleosts. The mRNA expression of striped snakehead fads also showed an ontogenic-related increase in expression in 0-20 days after hatch larva. Using ISH, we localized the presence of fads in larva brain, liver and intestinal tissues.
School of Biological Sciences, Universiti Sains Malaysia, 11800, Minden, Penang, Malaysia.
Annette
A
Jaya-Ram
Sairatul Dahlianis
SD
Ishak
Yee-Ling
YL
Enyu
Meng-Kiat
MK
Kuah
Kah-Loon
KL
Wong
Alexander Chong
AC
Shu-Chien
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D044943:Fatty Acid Desaturases / Q000235:genetics* / Q000378:metabolism; D029941:Fish Proteins / Q000235:genetics* / Q000378:metabolism; D007814:Larva / Q000378:metabolism; D008969:Molecular Sequence Data; D010473:Perciformes / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D010802:Phylogeny; D012333:RNA, Messenger / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
D029941:Fish Proteins; D012333:RNA, Messenger; D044943:Fatty Acid Desaturases