The effect of bromfenvinphos and its impurities on human erythrocyte.
Bromfenvinphos - (E,Z)-O,O-diethyl-O-[1-(2,4-dichlorophenyl)-2-bromovinyl] phosphate (BFVF) is the insecticide elaborated in Poland, which has been used against Varroa destructor causing honey bees disease called as varroosis. The substances that are formed as a result of bromfenvinphos synthesis are dihydro-bromfenvinphos (O,O-diethyl O-[1-(2,4-dichlorophenyl)vinyl] phosphate); dibromo-bromfenvinphos (O,O-diethyl O-[1-(2,4-dichlorophenyl)-2,2-dibromovinyl] phosphate); 2,4-dichlorophenacyl bromide; 2,4-dichlorophenacylidene bromide and 2,4-dichlorophenacylidyne bromide. In this work, we evaluated the effect of these compounds on hemolysis and hemoglobin oxidation (met-Hb formation) in human erythrocytes. Moreover, the changes in the size (FSC-A) and the shape (SSC-A) of red blood cells were assessed using flow cytometry and phase contrast microscopy. It was proven that bromfenvinphos at concentrations ranging from 0.5 to 250 μM during 1h incubation did not change the parameters examined in human erythrocytes. Similarly, most of bromfenvinphos impurities did not increase hemolysis and methemoglobin level nor changed the size and shape of the erythrocytes. The exception was dibromo-bromfenvinphos, which changed the FSC-A and SSC-A parameters, as well as 2,4-dichlorophenacyl bromide which induced hemolysis, increased the level of met-Hb and changed erythrocytes morphology.
Department of Biophysics of Environmental Pollution, University of Łódź, Łódź, Poland. ipo@ipo.waw.pl
Bozena
B
Szatkowska
Bozena
B
Bukowska
Bogumiła
B
Huras
D056810:Acaricides / Q000737:chemistry / Q000633:toxicity*; D002478:Cells, Cultured; D002709:Chlorfenvinphos / Q000031:analogs & derivatives* / Q000737:chemistry / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000187:drug effects*; D006801:Humans; D015394:Molecular Structure
D056810:Acaricides; C014369:bromfenvinphos; D002709:Chlorfenvinphos