Electrochemical properties of myoglobin deposited on multi-walled carbon nanotube/ciprofloxacin film.
We report the direct electrochemical and electrocatalytic properties of myoglobin (MB) on a multi-walled carbon nanotube/ciprofloxacin (MWCNT/CF) film-modified electrode. A highly homogeneous MWCNT thin-film was prepared on an electrode surface using ciprofloxacin (CF) as a dispersing agent. MB was then electrochemically deposited onto the MWCNT/CF-modified electrode. The MB/MWCNT/CF film was characterized by scanning electron microscopy and UV-visible spectroscopy (UV-vis). UV-vis spectra confirmed that MB retained its original state on the MWCNT/CF film. Direct electrochemical properties of MB on the MWCNT/CF film were investigated by cyclic voltammetry. The formal potential and electron transfer rate constant were evaluated in pH 7.2 buffer solution as -0.327V and 300s(-1), respectively. In addition, the MB/MWCNT/CF-modified electrode showed excellent electrocatalytic properties for the reduction of hydrogen peroxide (H(2)O(2)). The MB/MWCNT/CF-modified electrode was used for the detection of H(2)O(2) at concentrations from 1×10(-6)M to 7×10(-4)M in pH 7.2 buffer solution. Overall, the MB/MWCNT/CF-modified electrode was very stable and has potential for development as a H(2)O(2) sensor.
Department of Materials and Mineral Resources Engineering, No. 1, Sec. 3, Chung-Hsiao East Rd., National Taipei University of Technology, Taipei, Taiwan. sakumar80@gmail.com
S Ashok
SA
Kumar
Sea-Fue
SF
Wang
Yu-Tsern
YT
Chang
His-Chuan
HC
Lu
Chun-Ting
CT
Yeh
D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology; D015374:Biosensing Techniques; D002384:Catalysis; D002939:Ciprofloxacin / Q000494:pharmacology*; D004563:Electrochemistry / Q000379:methods*; D006736:Horses; D006861:Hydrogen Peroxide / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008956:Models, Chemical; D009206:Myocardium / Q000378:metabolism; D009211:Myoglobin / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
D000890:Anti-Infective Agents; D009211:Myoglobin; D037742:Nanotubes, Carbon; D002939:Ciprofloxacin; D006861:Hydrogen Peroxide