Role of NF-kB, p53, and p21 in the regulation of TNF-α mediated apoptosis of lymphocytes.
The effect of recombinant TNF-α on programmed death of donor lymphocytes was studied in vitro. The proapoptotic effect of this cytokine is realized through transcription factors and cell cycle inhibitors. Incubation of lymphocytes with recombinant TNF-α revealed increased levels of NF-kB and p21 and reducted content of nonphosphorylated p53.
Siberian State Medical University, Russian Ministry of Health, Tomsk, Russia. mv@mail.ssmu.ru
N V
NV
Ryazantseva
V V
VV
Novitskii
O B
OB
Zhukova
A K
AK
Biktasova
O E
OE
Chechina
E V
EV
Sazonova
T T
TT
Radzivil
A N
AN
Wice
N Yu
NY
Chasovskikh
D000328:Adult; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000378:metabolism*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000187:drug effects* / Q000502:physiology; D008297:Male; D016328:NF-kappa B / Q000378:metabolism*; D011994:Recombinant Proteins / Q000494:pharmacology*; D018709:Statistics, Nonparametric; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology* / Q000502:physiology; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
D050759:Cyclin-Dependent Kinase Inhibitor p21; D016328:NF-kappa B; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D016159:Tumor Suppressor Protein p53