Metabolomic investigations of American oysters using H-NMR spectroscopy.
The Eastern oyster (Crassostrea virginica) is a useful, robust model marine organism for tissue metabolism studies. Its relatively few organs are easily delineated and there is sufficient understanding of their functions based on classical assays to support interpretation of advanced spectroscopic approaches. Here we apply high-resolution proton nuclear magnetic resonance ((1)H NMR)-based metabolomic analysis to C. virginica to investigate the differences in the metabolic profile of different organ groups, and magnetic resonance imaging (MRI) to non-invasively identify the well separated organs. Metabolites were identified in perchloric acid extracts of three portions of the oyster containing: (1) adductor muscle, (2) stomach and digestive gland, and (3) mantle and gills. Osmolytes dominated the metabolome in all three organ blocks with decreasing concentration as follows: betaine > taurine > proline > glycine > ß-alanine > hypotaurine. Mitochondrial metabolism appeared most pronounced in the adductor muscle with elevated levels of carnitine facilitating ß-oxidation, and ATP, and phosphoarginine synthesis, while glycogen was elevated in the mantle/gills and stomach/digestive gland. A biochemical schematic is presented that relates metabolites to biochemical pathways correlated with physiological organ functions. This study identifies metabolites and corresponding (1)H NMR peak assignments for future NMR-based metabolomic studies in oysters.
Joint Department of Biomedical Engineering, NC State University and UNC Chapel Hill, Chapel Hill, NC 27599, USA. tikunov@email.unc.edu
Andrey P
AP
Tikunov
Christopher B
CB
Johnson
Haakil
H
Lee
Michael K
MK
Stoskopf
Jeffrey M
JM
Macdonald
D000818:Animals; D001622:Betaine / Q000378:metabolism; D049896:Crassostrea / Q000378:metabolism*; D005753:Gastric Mucosa / Q000378:metabolism; D005880:Gills / Q000378:metabolism; D005998:Glycine / Q000378:metabolism; D008279:Magnetic Resonance Imaging; D009682:Magnetic Resonance Spectroscopy; D055442:Metabolome; D055432:Metabolomics / Q000379:methods*; D008928:Mitochondria / Q000378:metabolism; D023421:Models, Animal; D009132:Muscles / Q000378:metabolism; D010472:Perchlorates / Q000378:metabolism; D012137:Respiratory System / Q000378:metabolism; D013654:Taurine / Q000378:metabolism; D014481:United States
D010472:Perchlorates; D013654:Taurine; D001622:Betaine; D005998:Glycine