Water deficit increases stilbene metabolism in Cabernet Sauvignon berries.
The impact of water deficit on stilbene biosynthesis in wine grape (Vitis vinifera) berries was investigated. Water deficit increased the accumulation of trans-piceid (the glycosylated form of resveratrol) by 5-fold in Cabernet Sauvignon berries but not in Chardonnay. Similarly, water deficit significantly increased the transcript abundance of genes involved in the biosynthesis of stilbene precursors in Cabernet Sauvignon. Increased expression of stilbene synthase, but not that of resveratrol-O-glycosyltransferase, resulted in increased trans-piceid concentrations. In contrast, the transcript abundance of the same genes declined in Chardonnay in response to water deficit. Twelve single nucleotide polymorphisms (SNPs) were identified in the promoters of stilbene synthase genes of Cabernet Sauvignon, Chardonnay, and Pinot Noir. These polymorphisms resulted in eight changes within the predicted cis regulatory elements in Cabernet Sauvignon and Chardonnay. These results suggest that cultivar-specific molecular mechanisms might exist that control resveratrol biosynthesis in grapes.
Department of Biochemistry and Molecular Biology, University of Nevada, Reno, Nevada 89557, USA.
Laurent G
LG
Deluc
Alain
A
Decendit
Yorgos
Y
Papastamoulis
Jean-Michel
JM
Mérillon
John C
JC
Cushman
Grant R
GR
Cramer
D000217:Acyltransferases / Q000235:genetics / Q000378:metabolism; D005638:Fruit / Q000737:chemistry / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D010936:Plant Extracts / Q000032:analysis / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D020641:Polymorphism, Single Nucleotide; D013267:Stilbenes / Q000032:analysis / Q000378:metabolism*; D027843:Vitis / Q000737:chemistry / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D014867:Water / Q000378:metabolism*
D010936:Plant Extracts; D010940:Plant Proteins; D013267:Stilbenes; D014867:Water; D000217:Acyltransferases; C022674:stilbene synthase