Cofactor trapping, a new method to produce flavin mononucleotide.
We have purified flavin mononucleotide (FMN) from a flavoprotein-overexpressing Escherichia coli strain by cofactor trapping. This approach uses an overexpressed flavoprotein to trap FMN, which is thus removed from the cascade regulating FMN production in E. coli. This, in turn, allows the isolation of highly pure FMN.
Institut für Molekulare Enzymtechnologie, Heinrich-Heine Universität Düsseldorf, Forschungszentrum Jülich, D-52426 Jülich, Germany. u.krauss@fz-juelich.de
Ulrich
U
Krauss
Vera
V
Svensson
Astrid
A
Wirtz
Esther
E
Knieps-Grünhagen
Karl-Erich
KE
Jaeger
D001709:Biotechnology / Q000379:methods; D003067:Coenzymes / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D005486:Flavin Mononucleotide / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism*; D005420:Flavoproteins / Q000737:chemistry / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial
D003067:Coenzymes; D029968:Escherichia coli Proteins; D005420:Flavoproteins; D005486:Flavin Mononucleotide