Metalloproteomics: forward and reverse approaches in metalloprotein structural and functional characterization.
About one-third of all proteins are associated with a metal. Metalloproteomics is defined as the structural and functional characterization of metalloproteins on a genome-wide scale. The methodologies utilized in metalloproteomics, including both forward (bottom-up) and reverse (top-down) technologies, to provide information on the identity, quantity, and function of metalloproteins are discussed. Important techniques frequently employed in metalloproteomics include classical proteomic tools such as mass spectrometry and 2D gels, immobilized-metal affinity chromatography, bioinformatic sequence analysis and homology modeling, X-ray absorption spectroscopy and other synchrotron radiation based tools. Combinative applications of these techniques provide a powerful approach to understand the function of metalloproteins.
Center for Proteomics and Bioinformatics, Case Western Reserve University, 10900 Euclid Ave, BRB 113, Cleveland, OH 44106, USA.
Wuxian
W
Shi
Mark R
MR
Chance
D000818:Animals; D019295:Computational Biology; D006801:Humans; D008667:Metalloproteins / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D011487:Protein Conformation; D040901:Proteomics / Q000379:methods*
D008667:Metalloproteins