Deposition of stearate-oleate rich seed fat in Mangifera indica is mediated by a FatA type acyl-ACP thioesterase.
Although the mechanism of accumulation of C8-C16 saturated fatty acids in seed oils has been well-studied, the control of stearic (C18:0) acid deposition in high stearate seed fat is still unclear. We investigated the mechanism that regulates high level of stearate and oleate (C18:1) accumulation in mango (Mangifera indica) seeds during its development, and examined the seed plastid extracts for induction of any specialized fatty acyl-ACP thioesterase (Fat) that may control this high level of deposition. Though the specificity of the Fat enzymes does not account directly for the fatty acid composition of mango seeds, our result suggested that an induced synthesis of a FatA type of thioesterase could be responsible for the high content of oleate and stearate in its seed fat. The major thioesterase from developing seed kernel was purified to near homogeneity, and characterized as a heat-labile, dimeric, neutral protein with relative substrate specificity of 100:35:1.8 towards oleoyl-, stearoyl- and palmitoyl-ACP, respectively. This enzyme was confirmed as Mi FatA by mass spectrometric analysis. Additionally, a heat-stable FatB type enzyme (Mi FatB) was also partially purified, with relative substrate specificity for the same substrates as 9:8.5:100, respectively. Mi FatA is an enzyme of great biotechnological interest because of its involvement in the regulation of stearate rich seed fat in mango.
Bose Institute, Calcutta, India.
Ashish
A
Bhattacharjee
Santosh K
SK
Ghosh
Krishnakali
K
Neogi
Aniruddha
A
Aich
Belinda
B
Willard
Michael
M
Kinter
Soumitra K
SK
Sen
Dolly
D
Ghosh
Sudhamoy
S
Ghosh
D000213:Acyl Carrier Protein / Q000378:metabolism*; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism; D031022:Mangifera / Q000201:enzymology* / Q000254:growth & development; D019301:Oleic Acid / Q000032:analysis / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012639:Seeds / Q000201:enzymology*; D013229:Stearic Acids / Q000032:analysis / Q000378:metabolism*; D013869:Thiolester Hydrolases / Q000378:metabolism*
D000213:Acyl Carrier Protein; D007527:Isoenzymes; D013229:Stearic Acids; D019301:Oleic Acid; C031183:stearic acid; D013869:Thiolester Hydrolases; C018748:oleoyl-(acyl-carrier-protein) hydrolase