The phosphoinositide 3-kinase Vps34p is required for pexophagy in Saccharomyces cerevisiae.
PIds (phosphoinositides) are phosphorylated derivatives of the membrane phospholipid PtdIns that have emerged as key regulators of many aspects of cellular physiology. We have discovered a PtdIns3P-synthesizing activity in peroxisomes of Saccharomyces cerevisiae and have demonstrated that the lipid kinase Vps34p is already associated with peroxisomes during biogenesis. However, although Vps34 is required, it is not essential for optimal peroxisome biogenesis. The function of Vps34p-containing complex I as well as a subset of PtdIns3P-binding proteins proved to be mandatory for the regulated degradation of peroxisomes. This demonstrates that PtdIns3P-mediated signalling is required for pexophagy.
Abteilung für Systembiochemie, Medizinische Fakultät der Ruhr-Universität Bochum, Bochum, Germany.
Silke
S
Grunau
Dorothee
D
Lay
Sabrina
S
Mindthoff
Harald W
HW
Platta
Wolfgang
W
Girzalsky
Wilhelm W
WW
Just
Ralf
R
Erdmann
D001343:Autophagy; D058546:Class III Phosphatidylinositol 3-Kinases / Q000235:genetics / Q000378:metabolism*; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal / Q000502:physiology*; D020675:Peroxisomes / Q000378:metabolism*; D018129:Phosphatidylinositol Phosphates / Q000235:genetics / Q000378:metabolism; D011485:Protein Binding; D021381:Protein Transport / Q000502:physiology; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000201:enzymology* / Q000502:physiology; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*
D018129:Phosphatidylinositol Phosphates; D029701:Saccharomyces cerevisiae Proteins; C055525:phosphatidylinositol 3-phosphate; D058546:Class III Phosphatidylinositol 3-Kinases