[Subcellular proteome analysis of immune or alcohol induced rat liver fibrosis].
OBJECTIVE
To study the mechanism of liver fibrogenesis and to find new non-invasive biomarkers.


METHODS
In this study, we used subcellular proteomic technology to study the plasma membrane proteins related to immune or alcohol induced liver fibrosis. Rat liver fibrosis models were induced by pig serum or alcohol injection. The liver fibrogenesis were detected by James's staining in the rat models after 2, 4, 6 and 8 weeks of treatment. The liver plasma membrane (PM) of the 2- and 8-week treatment model rats were enriched by two-step sucrose density gradient centrifugation. The purity of PM was verified by western blotting, and the plasma membrane proteins were extracted and analyzed by 2 DE. The differentially expressed proteins were identified by LC-MS/MS. Cellular location and function of these identified differential protein were classified.


RESULTS
Immune or alcohol induced liver fibrosis rat models were successfully established. Liver plasma membrane was significantly enriched after sucrose density ultracentrifugation treatment. 87 differential protein spots were find out by 2DE combined with LC-MS/MS from the liver plasma membrane proteins of the 2- and 8-week treatment rat models, which corresponded to 30 non-redundant proteins including annexin A2, keratin 8 and keratin 18.


CONCLUSIONS
A list of differentially expressed proteins relate to liver fibrosis were successfully identified. Differential proteins such as annexin A2, keratin 8 and keratin 18 could be new biomarkers for liver fibrosis diagnosis.
Shanghai Public Health Clinical Center Affiliated to Fudan University, Shanghai 201508, China.
Xiao-fang
XF
Jia
Xia
X
Peng
Yan-ling
YL
Feng
Hua
H
Yang
Zheng-hong
ZH
Yuan
Li-jun
LJ
Zhang
D000438:Alcohols / Q000009:adverse effects; D000818:Animals; D005260:Female; D053538:Keratin-18 / Q000378:metabolism; D053533:Keratin-8 / Q000378:metabolism; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D008103:Liver Cirrhosis / Q000139:chemically induced / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D008297:Male; D020543:Proteome / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
D000438:Alcohols; D053538:Keratin-18; D053533:Keratin-8; D020543:Proteome