Oxidative stress elevated DNA damage and homocysteine level in normal pregnant women in a segment of Pakistani population.
Maternal oxidative stress during pregnancy may impair fetal growth and help in the development of diseases in adulthood. The aim of current study was to assess total oxidation status (TOS), related parameters and their relationship to DNA damage (%) and homocysteine level in normal pregnant women in low-income participants. In a cross-sectional study healthy women were grouped as normal, while age matched nulliparous and singleton pregnancies were included for first, second and third trimester groups. TOS (P<0.01), melanodialdehyde (MDA) (P<0.001), aspartate aminotransferase (AST) (P<0.01), triiodothyronine (T3) (P<0.01), thyroxine (T4) (P<0.01), and homocysteine (P<0.001), in pregnant women were significantly higher as compared to normal healthy women. While serum total proteins (P<0.01), albumin (P<0.01) and total antioxidant status (TAS) (P<0.001) decreased significantly as compared to normal healthy women. Women in third trimester showed a significantly high level of body temperature (P<0.01), triglyceride (P<0.01), LDL-cholesterol (P<0.05), AST (P<0.01), T3 (P<0.01), homocysteine (P<0.001), TOS (P<0.01) and MDA (P<0.001) but a lower concentration of serum proteins, albumin and TAS at the end of the pregnancy. Pearson correlation indicated a positive relationship of homocysteine with triglycerides (P<0.027), TOS (P<0.01), MDA (P<0.035) and had a negative relationship with total protein (P<0.026). DNA damage was strongly related with T3 (P<0.008), TOS (P<0.02), MDA (P<0.037) and MBI (P<0.048) profiles of pregnant women. These changes were considered normal for pregnant women having optimum blood pressure and normal child birth. Hormonal influences and hemodilution may contribute towards the observed changes in this study.
Center for Agricultural Biochemistry and Biotechnology, University of Agriculture, Faisalabad, 38040, Pakistan.
Shazia A
SA
Bukhari
Muhammad Ibrahim
MI
Rajoka
Z
Z
Ibrahim
Fatima
F
Jalal
Shahid Mahboob
SM
Rana
Saeed A
SA
Nagra
D000328:Adult; D001219:Aspartate Aminotransferases / Q000097:blood; D001831:Body Temperature; D002784:Cholesterol / Q000097:blood; D003430:Cross-Sectional Studies; D004249:DNA Damage / Q000502:physiology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006710:Homocysteine / Q000097:blood*; D006801:Humans; D007124:Immunoenzyme Techniques; D018384:Oxidative Stress / Q000502:physiology*; D010154:Pakistan; D011247:Pregnancy; D013974:Thyroxine / Q000097:blood; D014284:Triiodothyronine / Q000097:blood
D014284:Triiodothyronine; D006710:Homocysteine; D002784:Cholesterol; D001219:Aspartate Aminotransferases; D013974:Thyroxine