Semi-synthesis of glycoproteins from E. coli through native chemical ligation.
Sufficient quantities of homogeneous samples of post-translationally modified proteins are often not readily available from biological sources to facilitate structure-function investigations. Native chemical ligation (NCL) is a convenient method for the production of biologically active proteins from smaller fragments. Such an approach allows protein modifications to be introduced in a controlled fashion into smaller peptide fragments which are amenable to total chemical synthesis. These fragments of defined sequence and structure can be elaborated to full-length proteins through NCL reactions with suitable components derived from bacterial origin. This report describes methods for the bacterial production of components for NCL and their use in typical reactions.
Department of Chemistry, Christopher Ingold Laboratories, University College London, London, WC1H 0AJ, UK. jrichard@sgul.ac.uk
Jonathan P
JP
Richardson
Derek
D
Macmillan
D004926:Escherichia coli; D006023:Glycoproteins / Q000096:biosynthesis* / Q000737:chemistry*; D011499:Protein Processing, Post-Translational; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000737:chemistry*
D006023:Glycoproteins; D011994:Recombinant Proteins