A simple reverse genetics approach to elucidating the biosynthetic pathway of nocathiacin.
Genomic library screening and genome mining are currently employed to identify biosynthetic gene clusters of thiopeptides. To elucidate the biosynthetic pathway of nocathiacin, we present a new approach with the application of simple reverse genetics. A relationship between structural features of thiopeptides and their biosynthetic pathways is established and is a starting point for speedily elucidating biosynthetic genes of various ribosomally-synthesized bioactive peptides with diverse modifications.
Laboratory of Chemical Biology, China Pharmaceutical University, 24 Tongjia Street, Nanjing, Jiangsu Province, 210009, People's Republic of China.
Maochen
M
Wei
Jing
J
Deng
Shuzhen
S
Wang
Nan
N
Liu
Yijun
Y
Chen
D000595:Amino Acid Sequence; D053898:Biosynthetic Pathways / Q000235:genetics / Q000502:physiology*; D016386:Chromosome Walking; D008969:Molecular Sequence Data; D015394:Molecular Structure; D010456:Peptides, Cyclic / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D010456:Peptides, Cyclic