Determination of enzymatic activities and metabolites in microliter sample size.
A new generation of spectrophotometers able to measure a wide range of absorbance in microliter aliquots is currently used for the determination of DNA, RNA, and proteins. The object of this article is to show that these instruments could be easily adapted for routine evaluation of enzymes and metabolites in 1-2-microl volumes of biological samples.
Departamento de Bioquímica, Facultad de Medicina, Instituto de Investigaciones Biomédicas "Alberto Sols" CSIC-UAM, 28029 Madrid, Spain. antonio.sillero@uam.es
Maria A Günther
MA
Sillero
Anabel de
Ad
Diego
Gemma
G
Rodríguez-Tarduchy
Antonio
A
Sillero
D000243:Adenosine Deaminase / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D057075:Enzyme Assays / Q000379:methods*; D004798:Enzymes / Q000378:metabolism*; D005240:Feasibility Studies; D019298:Glucose-6-Phosphate / Q000378:metabolism; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D057230:Limit of Detection; D016014:Linear Models
D004798:Enzymes; D019298:Glucose-6-Phosphate; D005954:Glucosephosphate Dehydrogenase; D002374:Catalase; D000243:Adenosine Deaminase