Spatiotemporally controlled contraction of micropatterned skeletal muscle cells on a hydrogel sheet.
We have developed gel sheet-supported C(2)C(12) myotube micropatterns and combined them with a microelectrode array chip to afford a skeletal muscle cell-based bioassay system. Myotube line patterns cultured on a glass substrate were transferred with 100% efficiency to the surface of fibrin gel sheets. The contractile behavior of each myotube line pattern on the gel was individually controlled by localized electrical stimulation using microelectrode arrays that had been previously modified with electropolymerized poly(3,4-ethylenedioxythiophene) (PEDOT). We successfully demonstrated fluorescent imaging of the contraction-induced translocation of the glucose transporter, GLUT4, from intracellular vesicles to the plasma membrane of the myotubes. This device is applicable for the bioassay of contraction-induced metabolic alterations in a skeletal muscle cell.
Department of Bioengineering and Robotics, Graduate School of Engineering, Tohoku University, 6-6-01 Aramaki, Aoba-ku, Sendai, 980-8579, Japan.
Kuniaki
K
Nagamine
Takeaki
T
Kawashima
Soichiro
S
Sekine
Yuichiro
Y
Ido
Makoto
M
Kanzaki
Matsuhiko
M
Nishizawa
D000818:Animals; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000737:chemistry; D002460:Cell Line; D004558:Electric Stimulation; D005947:Glucose / Q000378:metabolism; D051275:Glucose Transporter Type 4 / Q000378:metabolism; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D056656:Lab-On-A-Chip Devices; D051379:Mice; D008839:Microelectrodes; D018485:Muscle Fibers, Skeletal / Q000166:cytology*; D032448:Myoblasts, Skeletal / Q000166:cytology*; D011108:Polymers / Q000737:chemistry; D021381:Protein Transport / Q000502:physiology
D019086:Bridged Bicyclo Compounds, Heterocyclic; D051275:Glucose Transporter Type 4; D011108:Polymers; C121383:poly(3,4-ethylene dioxythiophene); D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D005947:Glucose