Concise synthesis of catechin probes enabling analysis and imaging of EGCg.
A concise synthesis of APDOEGCg (3) was accomplished. Due to the reactivity of its amine group, the compound could be easily converted to the fluorescein probe 21 and immunogen probe 22 efficiently. We then demonstrated the usefulness of the probes for imaging studies and the generation of antibodies.
School of Pharmaceutical Sciences, University of Shizuoka and Global COE Program, 52-1 Yada, Suruga-ku, Shizuoka-shi, Shizuoka 422-8526, Japan.
Atsushi
A
Yoshida
Yasuo
Y
Hirooka
Yusuke
Y
Sugata
Mariko
M
Nitta
Tamiko
T
Manabe
Shunsuke
S
Ido
Kouki
K
Murakami
Repon Kumer
RK
Saha
Takashi
T
Suzuki
Motohiro
M
Ohshima
Akira
A
Yoshida
Kunihiko
K
Itoh
Kosuke
K
Shimizu
Naoto
N
Oku
Takumi
T
Furuta
Tomohiro
T
Asakawa
Toshiyuki
T
Wakimoto
Toshiyuki
T
Kan
D000588:Amines / Q000737:chemistry; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D002392:Catechin / Q000031:analogs & derivatives* / Q000032:analysis / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D042783:Endothelial Cells / Q000166:cytology / Q000648:ultrastructure; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D013662:Tea / Q000737:chemistry*; D014471:Umbilical Veins / Q000166:cytology / Q000648:ultrastructure
D000588:Amines; D000975:Antioxidants; D013662:Tea; D002392:Catechin; C045651:epigallocatechin gallate