Identification of the transcriptional promoters in the proximal regions of human microRNA genes.
To identify the transcriptional promoters in the proximal regions of human microRNA (miRNA) genes, we analyzed the 5' flanking regions of intergenic miRNAs and intronic miRNAs. With the TSSG program prediction, we found that the ratio of intronic-s miRNA genes with a least one promoter was significantly lower than those of intergenic miRNA genes and intronic-a miRNA genes. More than half of the miRNA genes have only one promoter and less than 20% of the miRNA genes have more than three promoters in the 5-kb upstream regions. All potential promoters are randomly distributed within these regions. Approximately 60% of the miRNA promoters have a TATA-like box, being significantly higher than that of all human promoters. Luciferase reporter assays showed that 22 of the 30 promoters drove gene expression in HEK-293 cells, indicating a high accuracy of the promoter prediction. This study lays a foundation for future investigation into the transcriptional regulatory mechanisms of human miRNA genes.
Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and the Ministry of Education of China, Institute of Neuroscience and the Second Affiliated Hospital of Guangzhou Medical University, 250 Chang-gang-dong Road, Guangzhou, 510260, China. longys1972@yahoo.com.cn
Yue-Sheng
YS
Long
Guang-Fei
GF
Deng
Xun-Sha
XS
Sun
Yong-Hong
YH
Yi
Tao
T
Su
Qi-Hua
QH
Zhao
Wei-Ping
WP
Liao
D020029:Base Pairing / Q000235:genetics; D015894:Genome, Human / Q000235:genetics; D057809:HEK293 Cells; D006801:Humans; D008156:Luciferases / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics*; D011401:Promoter Regions, Genetic; D016385:TATA Box / Q000235:genetics; D014158:Transcription, Genetic
D035683:MicroRNAs; D008156:Luciferases