Standard practice for cell sorting in a BSL-3 facility.
Over the past decade, there has been a rapid growth in the number of BSL-3 and BSL-4 laboratories in the USA and an increase in demand for infectious cell sorting in BSL-3 laboratories. In 2007, the International Society for Advancement of Cytometry (ISAC) Biosafety Committee published standards for the sorting of unfixed cells and is an important resource for biosafety procedures when performing infectious cell sorting. Following a careful risk assessment, if it is determined that a cell sorter must be located within a BSL-3 laboratory, there are a variety of factors to be considered prior to the establishment of the laboratory. This chapter outlines procedures for infectious cell sorting in a BSL-3 environment to facilitate the establishment and safe operation of a BSL-3 cell sorting laboratory. Subjects covered include containment verification, remote operation, disinfection, personal protective equipment (PPE), and instrument-specific modifications for enhanced aerosol evacuation.
Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.
Stephen P
SP
Perfetto
David R
DR
Ambrozak
Richard
R
Nguyen
Mario
M
Roederer
Richard A
RA
Koup
Kevin L
KL
Holmes
D000336:Aerosols / Q000592:standards; D003264:Containment of Biohazards / Q000592:standards; D005161:Facility Regulation and Control / Q000592:standards; D005434:Flow Cytometry / Q000295:instrumentation / Q000379:methods* / Q000592:standards*; D007753:Laboratories / Q000592:standards*; D011482:Protective Devices / Q000592:standards; D017751:Safety Management / Q000592:standards*
D000336:Aerosols