[Change of structural maintenance of chromosome (SMC)1, SMC3, Separase and Securin expression in BEAS-2B malignant transformation cell induced by coal tar pitch smoke extracts].
OBJECTIVE
to study the role of structural maintenance of chromosome (SMC)1, SMC3, Separase and Securin in tumorgenesis that contact with coal tar pitch.


METHODS
the BEAS-2B cells was induced by coal tar pitch smoke extracts to form malignant transformation cell model in vitro. The gene expression levels of mRNA were assessed by real-time quantitative RT-PCR, and the protein expression variation were determined by cell culture overslip of immunohistochemical methods.


RESULTS
in malignant transformation cells, the mRNA and the protein expression level of SMC1 gene was not statistically significantly different compared with the BEAS-2B group and DMSO group (P > 0.05); SMC3 and Separase was increased and Securin was decreased (P < 0.05), while the difference between other two control groups was not significant (P > 0.05).


CONCLUSIONS
the up expression level of SMC3 and Separase and the down expression level of Securin are involved in the process that evolves into malignant transformation in bronchial epithelial cells BEAS-2B induced by coal tar pitch smoke extracts.
College of Public Health, Zhengzhou University, Zhengzhou 450001, China.
Zhi-Tao
ZT
Li
Wei
W
Wang
Yong
Y
Zhao
Li-Xia
LX
Wang
Han-Song
HS
Zhu
Wei-Dong
WD
Wu
Yi-Ming
YM
Wu
D001980:Bronchi / Q000166:cytology; D018797:Cell Cycle Proteins / Q000378:metabolism; D002460:Cell Line; D002461:Cell Line, Transformed / Q000166:cytology / Q000187:drug effects; D011508:Chondroitin Sulfate Proteoglycans / Q000378:metabolism; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism; D003033:Coal Tar / Q000633:toxicity*; D010450:Endopeptidases / Q000378:metabolism; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins / Q000378:metabolism; D064247:Separase; D012854:Sister Chromatid Exchange; D012906:Smoke / Q000009:adverse effects*; D000098550:Structural Maintenance of Chromosome Protein 1
D018797:Cell Cycle Proteins; D011508:Chondroitin Sulfate Proteoglycans; D002868:Chromosomal Proteins, Non-Histone; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; C112633:PTTG1IP protein, human; C492318:SMC3 protein, human; D012906:Smoke; D000098550:Structural Maintenance of Chromosome Protein 1; D003033:Coal Tar; D010450:Endopeptidases; C577987:ESPL1 protein, human; D064247:Separase