Perfluorooctane sulfonate induces apoptosis in N9 microglial cell line.
Perfluorooctane sulfonate (PFOS) is an environmental persistent acid found at low levels in human, wildlife, and environmental media samples. To study the apoptosis effects of PFOS on microglia, murine N9 cell line was used as a model in current research. The results showed that PFOS could reduce the cell viability significantly, and the cellular apoptosis induced by PFOS was closely accompanied with dissipation of mitochondria membrane potential, upregulation messenger RNAs (mRNAs) of p53, Bax, caspase 9, and caspase 3, and decreased expression of Bcl-2 mRNA. These results suggested that PFOS could disturb homeostasis of N9 cells, impact mitochondria, and affect gene expression of apoptotic regulators, all of which resulted in a start-up of apoptosis.
Ministry of Education Key Laboratory of Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Ling
L
Zhang
Yuan-yuan
YY
Li
Huai-cai
HC
Zeng
Miao
M
Li
Yan-Jian
YJ
Wan
Hermann J
HJ
Schluesener
Zhi-yuan
ZY
Zhang
Shun-qing
SQ
Xu
D017738:Alkanesulfonic Acids / Q000633:toxicity*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D015415:Biomarkers; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D053453:Caspase 9 / Q000235:genetics / Q000378:metabolism; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003603:Cytotoxins / Q000633:toxicity; D005466:Fluorocarbons / Q000633:toxicity*; D005786:Gene Expression Regulation; D006706:Homeostasis; D051379:Mice; D017628:Microglia / Q000166:cytology* / Q000473:pathology; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D051336:Mitochondrial Membranes / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000235:genetics / Q000378:metabolism
D017738:Alkanesulfonic Acids; C494827:Bax protein, mouse; D015415:Biomarkers; D003603:Cytotoxins; D005466:Fluorocarbons; D012333:RNA, Messenger; D051028:bcl-2-Associated X Protein; C076994:perfluorooctane sulfonic acid; C505409:Casp3 protein, mouse; C506434:Casp9 protein, mouse; D053148:Caspase 3; D053453:Caspase 9