[Analysis of human papillomavirus type 61, 83 and 84 MY fragments and preparation of standard samples].
OBJECTIVE
To investigate the mutations in nucleotide and amino acid level in HPV61, 83 and 84 Shanxi isolates.


METHODS
Amplified fragments of HPV61, 83 and 84 from human papillomavirus (Human papillomavirus, HPV) molecular epidemiologic survey of Shanxi Province using HPV consensus primers MY09/11 were cloned in pMD18-T vector, and the plasmids were sequenced, then nucleotide sequences and amino acid sequences were analyzed.


RESULTS
HPV61 and HPV83 isolates were consistent with reference strains U31793 and AF151983 in nucleotide sequences; four mutations of nucleotide (C6760T, T6931C, T6951C and C6987A) were found in HPV84 isolate compared with reference strain AF293960, among which C6987A resulted in D441E and the amplified sensitivity of standard sample of HPV61 using primers MY09/11 was higher than that of HPV83 and 84.


CONCLUSIONS
HPV61 and HPV83 isolates were consistent with reference strains, four mutations of nucleotide and one mutation of amino acid were found in HPV84,the amplified sensitivity of standard sample of HPV61 using primers MY09/11 was the highest among those three isolates.
Department of Gynecology & Obstetrics, Tianjin Medical University General Hospital, China.
Bao-Chen
BC
Wang
Xiao-Li
XL
Wang
Feng-Xia
FX
Xue
Hong-Tu
HT
Liu
D001483:Base Sequence; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D027383:Papillomaviridae / Q000235:genetics*; D030361:Papillomavirus Infections / Q000821:virology*; D012680:Sensitivity and Specificity; D012689:Sequence Homology, Nucleic Acid