Detection of urinary biomarkers for early diagnosis of acute renal allograft rejection by proteomic analysis.
Acute allograft rejection has been recognized as a major impediment to improved success in renal transplantation. Timely detection and control of rejection are very important for the improvement in long-term renal allograft survival. Thus, biomarkers for early diagnosis of acute rejection are required urgently to clinical medication. This study seeks to search for such biomarker candidates by comparing patients' pre-treatment urinary protein profiling with their post-treatment urinary protein profiling. A total of 15 significantly and consistently down-regulated protein candidates were identified. Among them, alpha-1-antichymotrypsin precursor (AACT), tumor rejection antigen gp96 (GP96) and Zn-Alpha-2-Glycoprotein (ZAG) were selected for further analysis. The results indicated that Western Blot assay of AACT, GP96 and ZAG had advanced the diagnosis time of acute renal rejection by 3 days, compared with current standard clinical observation and laboratory examination. Furthermore, the double-blind detection revealed that the accuracy, sensitivity and specificity of the diagnosis of acute renal rejection of AACT, GP96 and ZAG were 66.67%/100%/60%, 83.33%/100%/80% and 66.67%/100%/60%, respectively, and 100%/100%/100% in combination. In conclusion, urinary protein AACT, GP96 and ZAG could be a set of potential biomarkers for early non-invasive diagnosis of the acute rejection after renal transplantation.
Chongqing Key Laboratory for Diseases Proteomics, Southwest Hospital, Third Military Medical University, Chongqing, P. R. China; State Key Laboratory of Trauma, Burns and Combined Injury, Southwest Hospital, Third Military Medical University, Chongqing, P. R. China; Institute of Burn Research, Southwest Hospital, Third Military Medical University, Chongqing, P. R. China.
Xiongfei
X
Jia
Chengjun
C
Gan
Ke
K
Xiao
Weifeng
W
He
Tao
T
Zhang
Cibing
C
Huang
Xiongfei
X
Wu
Gaoxing
G
Luo
Xiaojuan
X
Wang
Jie
J
Hu
Jiangling
J
Tan
Xiaorong
X
Zhang
Peter Mose
PM
Larsen
Jun
J
Wu