Quantitative analysis of a prostate-specific antigen in serum using fluorescence immunochromatography.
A quantitative analysis of prostate-specific antigen (PSA) in samples of human blood serum by fluorescence immunochromatography using monoclonal antibodies to PSA was developed. The fluorescence immunochromatographic analysis system is composed of anti-PSA-monoclonal antibody (mAb), fluorescence conjugates in detection solution, a immunochromatographic assay strip, and a laser fluorescence scanner. A fluorescence immunochromatographic analysis system was employed to detect PSA on the basis of the area ratio between the control line and the test line of the strip. Under optimal conditions, the area ratio was proportional to PSA concentration ranging from 0.72 to 46.0 ng/mL with a detection limit of 0.72 ng/mL.
Department of Chemistry, Kangwon National University, ChunChon, South Korea.
Jisun
J
Yoo
Young Mee
YM
Jung
Jong Hoon
JH
Hahn
Dongjin
D
Pyo
D000911:Antibodies, Monoclonal; D002846:Chromatography, Affinity / Q000379:methods*; D005453:Fluorescence; D006801:Humans; D057230:Limit of Detection; D008297:Male; D017430:Prostate-Specific Antigen / Q000097:blood*; D011471:Prostatic Neoplasms / Q000175:diagnosis
D000911:Antibodies, Monoclonal; D017430:Prostate-Specific Antigen