Physicochemical properties, sealing ability, bond strength and cytotoxicity of a new dimethacrylate-based root canal sealer.
OBJECTIVE
Resin-based root canal sealer can bond to dentin and establish a hermetic seal. The aim of this study was to evaluate the physicochemical properties, sealing ability, cytotoxicity and bond strength of a new resin-based root canal sealer (NRCS).


METHODS
The physicochemical properties were assessed by the flow, setting time, solubility, film thickness, radiopacity and dimensional changes. Sixty premolar root samples were filled with either Resilon/NRCS or Resilon/Epiphany and sectioned perpendicularly at the long axis at 2 mm below the cemento-enamel junction into 1-mm serial slices. The bond strength was tested by a universal testing machine. The glucose microleakage model was used to test the sealing ability. Elutes of NRCS and Epiphany were co-cultured with human periodontal ligament cells to test the cytotoxicity.


RESULTS
All the physicochemical properties of NRCS conformed to ISO 6876:2001(E). The root samples filled with Resilon/NRCS had significantly less leakage (p < 0.01) and greater bond strength (p < 0.001) than the Resilon/Epiphany group had. Environmental scanning electron microscopy showed that Resilon/NRCS filling material was intimately bonded to the root dentin. Although NRCS was slightly toxic to human periodontal ligament cells, its cytotoxicity was significantly less than that of Epiphany (p < 0.01)


CONCLUSIONS
NRCS has better physicochemical and sealing properties, as well as lower cytotoxicity and microleakage than Epiphany has.
Department of Endodontics and Conservative Dentistry, Guanghua College of Stomatology, Sun Yat-sen University, Guangzhou, China.
Zhengmei
Z
Lin
Junqi
J
Ling
Junyan
J
Fang
Fang
F
Liu
Jingwei
J
He
D000293:Adolescent; D000328:Adult; D002126:Calcium Hydroxide / Q000737:chemistry; D001840:Dental Bonding / Q000379:methods*; D003763:Dental Leakage; D003786:Dental Pulp Cavity; D003799:Dental Stress Analysis; D003804:Dentin; D017220:Dentin-Bonding Agents / Q000737:chemistry* / Q000633:toxicity; D005260:Female; D006801:Humans; D008297:Male; D020366:Methylmethacrylate / Q000737:chemistry* / Q000633:toxicity; D008855:Microscopy, Electron, Scanning; D010513:Periodontal Ligament / Q000166:cytology; D012387:Root Canal Filling Materials / Q000737:chemistry* / Q000633:toxicity; D012390:Root Canal Therapy; D013718:Tensile Strength; D013997:Time Factors; D055815:Young Adult
D017220:Dentin-Bonding Agents; D012387:Root Canal Filling Materials; D020366:Methylmethacrylate; D002126:Calcium Hydroxide