The yctCBA operon of Yersinia ruckeri, involved in in vivo citrate uptake, is not required for virulence.
A three-gene operon, named yctCBA (Yersinia citrate transporter), induced by citrate and repressed by glucose was identified from a previously selected in vivo-induced (ivi) clone in the fish pathogen Yersinia ruckeri. Interestingly, despite being an ivi clone, the drastic growth reduction of the yctC mutant in the presence of citrate, and the relatively high content of this compound in rainbow trout serum, the operon was not required for virulence.
Área de Microbiología, Departamento de Biología Funcional, Facultad de Medicina, IUBA, Universidad de Oviedo, 33006 Oviedo, Asturias, Spain.
Roberto
R
Navais
Jessica
J
Méndez
Pilar
P
Reimundo
David
D
Pérez-Pascual
Esther
E
Gómez
José A
JA
Guijarro
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D019343:Citric Acid / Q000378:metabolism*; D005393:Fish Diseases / Q000382:microbiology*; D008969:Molecular Sequence Data; D009154:Mutation; D017686:Oncorhynchus mykiss / Q000382:microbiology*; D009876:Operon; D017422:Sequence Analysis, DNA; D014774:Virulence; D015009:Yersinia Infections / Q000382:microbiology / Q000662:veterinary*; D044124:Yersinia ruckeri / Q000235:genetics / Q000254:growth & development / Q000378:metabolism / Q000472:pathogenicity*
D001426:Bacterial Proteins; D019343:Citric Acid