HtpS, a novel immunogenic cell surface-exposed protein of Streptococcus suis, confers protection in mice.
Streptococcal histidine triad protein was identified recently as a cell surface-associated protein family. Five members of this family (PhtA, PhtB, PhtD, PhtE and HtpA), derived from Streptococcus pneumoniae and Streptococcus pyogenes, have been shown as antigens that confer protection to the host on infection. In this report, a gene sequence highly homologous to htpA and phtD (designated htpS, the histidine triad protein of Streptococcus suis) was identified from S. suis 2 Chinese strain 05ZYH33. Our data revealed that htpS is extremely conserved in S. suis 2 and widely distributed in 83% (29/35) of 35 S. suis serotypes. It was also demonstrated by Western blot and flow cytometry that HtpS is a cell surface-associated protein that was expressed during S. suis 2 infection. An antibody against HtpS could increase the deposition of human complement 3 on S. suis 2 and also enhance the clearance of S. suis 2 in whole blood. In addition, mice could be immunized against S. suis 2 infection and were well protected after immunization with recombinant HtpS.
Department of Epidemiology, Research Institute for Medicine of Nanjing Command, Nanjing, China The College of Life Science, Nanjing Normal University, Nanjing, China.
Zhuqing
Z
Shao
Xiuzhen
X
Pan
Xianfu
X
Li
Wenjing
W
Liu
Mingyue
M
Han
Changjun
C
Wang
Jing
J
Wang
Feng
F
Zheng
Min
M
Cao
Jiaqi
J
Tang
D000595:Amino Acid Sequence; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology*; D001770:Blood Bactericidal Activity; D015153:Blotting, Western; D003176:Complement C3 / Q000276:immunology; D017124:Conserved Sequence; D004195:Disease Models, Animal; D005260:Female; D005434:Flow Cytometry; D020869:Gene Expression Profiling; D008565:Membrane Proteins / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D011817:Rabbits; D016415:Sequence Alignment; D013290:Streptococcal Infections / Q000517:prevention & control / Q000662:veterinary*; D017011:Streptococcus suis / Q000235:genetics / Q000276:immunology*; D016019:Survival Analysis
D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D003176:Complement C3; D008565:Membrane Proteins