Preparation of protein nanoarray on silicon surface by atomic force microscopy nanofabrication.
Atomic force microscopy (AFM) with bias control is employed to fabricate oxidized nanopatterns on a silicon surface with a feature size as low as 50 nm. Nanopatterns made by a Pt/Ir coating probes have larger feature size than these made by the probe without, but the patterning speed is fast, 0.1 s per dot. 20 nm gold nanoparticles are immobilized on oxide nanopatterns to elucidate the dimensions of the nanoparticles on an oxide nanopattern. These patterning conditions are utilized to prepare a nanoarray for the immobilization of biotins to interact with free streptavidins. The resultant height of the biotin labeled on oxidized nanopattern is 0.93 +/- 0.1 nm and the combined height of biotin-streptavidin is 5.14 +/- 0.45 nm, as determined using the imaging functions of AFM. Based on the experimental results, a nano biochip of silicon dioxide can be utilized to monitor molecular interactions on the nanometer scale under static conditions and without the labeling of fluorescence dyes.
Department of Electrical Engineering, National Taiwan University, Taipei 10617, Taiwan.
Hui-Hsin
HH
Lu
Chii-Wann
CW
Lin
D018625:Microscopy, Atomic Force; D036103:Nanotechnology; D040081:Protein Array Analysis