[Study on diversity of Liriope spicata var. prolifera and its affinis species with ISSR method].
OBJECTIVE
To study the identification of Liriope spicata var. prolifera and its affinis species, which are difficult to be differentiated with routine method, based on ISSR molecular marker technology and explore their relationship.


METHODS
According to the pre-experiment, to design orthogonal experiment of four levels and five factors to optimize the PCR reaction system. To select randomly 80 primers from ISSR primers sequence table and then screen effective primers from the experiment trough PCR amplifying of all samples. Mark the samples with optimal ISSR conditions, and calculate the similarity coefficient between samples, then build the phylogenetic tree of these samples.


RESULTS
The study established a better ISSR reaction system and the PCR amplification procedure for Liriope spicata var. prolifera and its affinis species, screened out 9 effective primers and achieved ISSR electrophoretic spectrum and phylogenetic tree of 15 samples.


CONCLUSIONS
The established ISSR molecular marker techniques can be used for the researches of identification and genetic relationship for some Liriope and Ophiopogon species.
School of Chemical Engineering, Wuhan University of Technology, Wuhan 430079, China. lrx1125@126.com
Xia
X
Liu
Zheng
Z
Liu
Min
M
Yang
Ke-Li
KL
Chen
D017931:DNA Primers; D018744:DNA, Plant / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D005819:Genetic Markers; D031424:Liriope Plant / Q000145:classification / Q000235:genetics*; D018895:Microsatellite Repeats; D010802:Phylogeny; D018515:Plant Leaves / Q000235:genetics; D010946:Plants, Medicinal / Q000145:classification / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D015203:Reproducibility of Results
D017931:DNA Primers; D018744:DNA, Plant; D005819:Genetic Markers