Efficacy of self-assembled hydrogels composed of positively or negatively charged peptides as scaffolds for cell culture.
KASEA16(+) and KASEA16(-) peptides, the net charges of which are positive and negative, respectively, under a neutral condition could undergo self-assembly into nanofibers and form transparent hydrogels without peptide aggregation upon rapid pH neutralization. The numbers of NIH3T3 cells attached to the KASEA16(+) hydrogel and KASEA16(-) hydrogel were similar, and cells proliferated with time on both hydrogels. Cells on the KASEA16(+) hydrogel had spindle-like morphology, while cells on the KASEA16(-) hydrogel formed clusters without extending cytoplasmic processes. Comparison of differently charged peptides under a neutral condition suggested that the charges of the scaffolds should be taken into consideration for the best design and selection of scaffolds for cell culture. Since the KASEA16(+) peptide could form a stable hydrogel under a neutral condition and the hydrogel served as a scaffold for cell proliferation, the KASEA16(+) hydrogel will be a useful scaffold for cell culture.
Department of Veterinary Anatomy, School of Veterinary Medicine, Rakuno Gakuen University, 582, Midorimachi, Bunkyodai, Ebetsu, Hokkaido 069-8501, Japan.
Aya
A
Nagayasu
Hidenori
H
Yokoi
Jun A
JA
Minaguchi
Yoshinao Z
YZ
Hosaka
Hiromi
H
Ueda
Kazushige
K
Takehana
D000595:Amino Acid Sequence; D000818:Animals; D002448:Cell Adhesion; D049109:Cell Proliferation; D020100:Hydrogels / Q000737:chemistry*; D051379:Mice; D008969:Molecular Sequence Data; D041681:NIH 3T3 Cells; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D010455:Peptides / Q000737:chemistry*; D054457:Tissue Scaffolds / Q000737:chemistry*
D020100:Hydrogels; D010455:Peptides