Detecting and measuring free gadolinium in nanoparticles for MRI imaging.
This chapter describes a method for the measurement of free gadolinium in nanoparticle samples. Conjugation of a gadolinium-chelate to a nanoparticle allows the particle's distribution to be imaged via magnetic resonance imaging (MRI). Free (unchelated) gadolinium is a known toxin, being a heavy metal, and may contribute towards total gadolinium concentration. Determining the amount of free gadolinium is therefore an important aspect of the preclinical characterization of gadolinium-chelate MRI imaging agent nanoparticles.
Nanotechnology Characterization Laboratory, Advanced Technology Program, SAIC-Frederick, Inc., National Cancer Institute at Frederick, Frederick, MD, USA.
Jeffrey D
JD
Clogston
Anil K
AK
Patri
D000818:Animals; D002138:Calibration; D002614:Chelating Agents / Q000737:chemistry; D003287:Contrast Media / Q000032:analysis*; D005682:Gadolinium / Q000032:analysis*; D006801:Humans; D008279:Magnetic Resonance Imaging / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry*; D009942:Organometallic Compounds / Q000737:chemistry; D012015:Reference Standards; D013056:Spectrophotometry, Ultraviolet
D002614:Chelating Agents; D003287:Contrast Media; D009942:Organometallic Compounds; D005682:Gadolinium