Use of serum circulating CCNB2 in cancer surveillance.
Cyclin B2 (CCNB2), a member of the cyclin protein family, has been found to be up-regulated in human cancers. To evaluate the potential use of circulating CCNB2 in serum in cancer surveillance, we examined relative expression levels of serum circulating CCNB2 mRNA in 103 cancer patients, 19 normal controls, and 40 benign disease patients using real-time quantitative reverse transcriptase polymerase chain reaction. We found that the relative expression level of circulating CCNB2 mRNA in cancer patients was significantly higher (p&lt;0.0001) than that in normal controls and benign diseases group. Circulating CCNB2 mRNA level was significantly (p&lt;0.001) correlated with cancer stage and metastasis status. Receiver operating characteristic (ROC) analysis showed an area under the curve (AUC) of 0.87 and 0.83 (p&lt;0.05) in identifying cancer patients' metastasis status in lung and digestive tract cancer, respectively. Moreover, we observed that expression levels of circulating CCNB2 mRNA in cancer patients significantly decreased (p=0.0084) after their therapeutic treatments. These data suggest that detection of serum circulating CCNB2 mRNA may have potential clinical applications in screening and monitoring of metastasis and therapeutic treatments.
School of Life Sciences, Tsinghua University, Beijing - PR China.
Min-Li
ML
Mo
Zhao
Z
Chen
Jie
J
Li
Hai-Long
HL
Li
Qing
Q
Sheng
Hui-Yan
HY
Ma
Fang-Xin
FX
Zhang
Ya-Wei
YW
Hua
Xun
X
Zhang
Da-Qiang
DQ
Sun
Mei-Lin
ML
Xu
Run-Gui
RG
Niu
Jun-Pu
JP
Xu
Hui-Jing
HJ
Zuo
Min
M
Liu
Hai-Meng
HM
Zhou