Contributions of steroidogenic factor 1 to the transcription landscape of Y1 mouse adrenocortical tumor cells.
The contribution of steroidogenic factor 1 (SF-1) to the gene expression profile of Y1 mouse adrenocortical cells was evaluated using short hairpin RNAs to knockdown SF-1. The reduced level of SF-1 RNA was associated with global changes that affected the accumulation of more than 2000 transcripts. Among the down-regulated transcripts were several with functions in steroidogenesis that were affected to different degrees--i.e., Mc2r>Scarb1>Star≥Hsd3b1>Cyp11b1. For Star and Cyp11b1, the different levels of expression correlated with the amount of residual SF-1 bound to the proximal promoter regions. The knockdown of SF-1 did not affect the accumulation of Cyp11a1 transcripts even though the amount of SF-1 bound to the proximal promoter of the gene was reduced to background levels. Our results indicate that transcripts with functions in steroidogenesis vary in their dependence on SF-1 for constitutive expression. On a more global scale, SF-1 knockdown affects the accumulation of a large number of transcripts, most of which are not recognizably involved in steroid hormone biosynthesis.
Banting and Best Department of Medical Research, University of Toronto, 112 College St., Toronto, ON, Canada M5G 1L6. bernard.schimmer@utoronto.ca
Bernard P
BP
Schimmer
Jennivine
J
Tsao
Martha
M
Cordova
Sara
S
Mostafavi
Quaid
Q
Morris
Joshua O
JO
Scheys
D000306:Adrenal Cortex Neoplasms / Q000235:genetics*; D000818:Animals; D045744:Cell Line, Tumor; D002786:Cholesterol Side-Chain Cleavage Enzyme / Q000235:genetics; D047369:Chromatin Immunoprecipitation; D002999:Clone Cells; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D051379:Mice; D010641:Phenotype; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism; D013252:Steroid 11-beta-Hydroxylase / Q000235:genetics; D054339:Steroidogenic Factor 1 / Q000235:genetics / Q000378:metabolism*; D013256:Steroids / Q000096:biosynthesis; D014158:Transcription, Genetic; D014170:Transformation, Genetic; D000098682:Steroidogenic Acute Regulatory Protein
D010750:Phosphoproteins; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D054339:Steroidogenic Factor 1; D013256:Steroids; D000098682:Steroidogenic Acute Regulatory Protein; C515058:steroidogenic factor 1, mouse; D013252:Steroid 11-beta-Hydroxylase; D002786:Cholesterol Side-Chain Cleavage Enzyme