Different protein profile in amniotic fluid with nervous system malformations by surface-enhanced laser desorption-ionization/time-of-flight mass spectrometry (SELDI-TOF-MS) technology.
OBJECTIVE
To detect the distinct proteins in amniotic fluid (AF) between nervous system malformations fetuses and normal fetuses.


METHODS
Surface-enhanced laser desorption-ionization/time-of-flight mass spectrometry was used to characterize AF peptides in AF between nervous system malformations fetuses and normal fetuses. WCX2 protein chips were used to characterize AF peptides in AF. Protein chips were examined in a PBSIIC protein reader, the protein profiling was collected by ProteinChip software version 3.1 (Ciphergen Biosystems, Fremont, CA, USA) and analyzed by Biomarker Wizard software (Ciphergen Biosystems). Nine distinct proteins were identified in AF between nervous system malformations fetuses and normal fetuses.


RESULTS
Compared with the control group, three proteins with m/z 4967.5 Da, 5258.0 Da, and 11,717.0 Da were down-regulated, and six proteins with m/z 2540.4 Da, 3107.1 Da, 3396.8 Da, 4590.965 Da, 5589.2 Da and 6429.4 Da up-regulated in nervous system malformations fetuses.


CONCLUSIONS
The results suggest that there are distinct proteins in protein profiling of AF between nervous system malformations fetuses and normal fetuses.
Department of Ultrasound Basic Medicine, Qilu Hospital, Shandong University, Shandong Province, China.
Zhe
Z
Ma
Cun
C
Liu
Biping
B
Deng
Shaogang
S
Dong
Guowei
G
Tao
Xinfeng
X
Zhan
Chuner
C
Wang
Shaoping
S
Liu
Xun
X
Qu
D000328:Adult; D000653:Amniotic Fluid / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D030562:Databases, Protein; D005260:Female; D005315:Fetal Diseases / Q000175:diagnosis / Q000378:metabolism; D006801:Humans; D009421:Nervous System Malformations / Q000175:diagnosis* / Q000378:metabolism; D010449:Peptide Mapping; D011247:Pregnancy; D011296:Prenatal Diagnosis; D015203:Reproducibility of Results; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D055815:Young Adult
D015415:Biomarkers