The effect of two different polyethylene glycol (PEG) derivatives on the immunological response of PEG grafted pancreatic islets.
Islet transplantation is one of the promising ways to treat diabetes. To reduce the immune system response, several methods have been developed, a novel one being the grafting of methoxy polyethylene glycol (mPEG) derivatives onto collagen capsules of islets. In this study, the effects of the first and second generations of activated mPEG on the immunological response of polyethylene glycol (PEG) grafted pancreatic islets were studied. mPEG-Succinimidyl carbonate (mPEG-SC) and mPEG-succinimidyl propionic acid (mPEG-SPA) (with nominal molecular weight 5 kDa), typical of the first and second generations of activated mPEG, were selected, respectively. Both activated mPEGs did not affect the morphology, viability, or functionality of PEGylated islets compared to free islets (naked islets). The amount of IL-2 secreted from lymphocytes co-cultured with mPEG-SPA grafted islets (131.83 ± 15.28 pg/ml) was not significantly different from that with mPEG-SC grafted islets (156.09 ± 27.94 pg/ml). These results indicated that both mPEG-SC and mPEG-SPA had the same effect for camouflaging Langerhans islets, but the former is more suitable due to its easier synthesis process.
Biotechnology Group, Department of Chemical Engineering, Faculty of Engineering, Tarbiat Modares University, P.O. Box 14115-143, Tehran, Iran.
Hamideh
H
Aghajani-Lazarjani
Ebrahim
E
Vasheghani-Farahani
Seyed Abbas
SA
Shojaosadati
Sameereh
S
Hashemi-Najafabadi
Saleh
S
Zahediasl
Taki
T
Tiraihi
Fatemeh
F
Atyabi
D000818:Animals; D002478:Cells, Cultured; D006789:Host vs Graft Reaction / Q000187:drug effects*; D016381:Islets of Langerhans Transplantation / Q000276:immunology*; D008214:Lymphocytes; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011092:Polyethylene Glycols / Q000494:pharmacology*; D051381:Rats
C556225:methoxypolyethylene glycol-succinimidyl-propionic acid ester; C099846:succinimidyl carbonate poly(ethylene glycol) monomethyl ether; D011092:Polyethylene Glycols