Reversible conformational transitions of a polymer brush containing boronic acid and its interaction with mucin glycoprotein.
Reversible changes of the height of a polymer brush containing phenylboronic acid were studied. The polymer brush thickness underwent reversible changes of 0.5-1 nm, in response to the changes in composition of the contacting aqueous phase from deionized water to bicarbonate buffer and vice versa, apparently due to the conformational transition of the weak polyelectrolyte to the more extended electrically charged state. Adsorption of mucin glycoprotein to the polymer brush took place due to boronate/sugar interactions between the glycoprotein and the graft copolymer and resulted in further increase of the brush height by ca. 1.5 nm, as observed by means of spectral correlation spectroscopy and ellipsometry.
Protista Biotechnology AB, IDEON, SE-22370, Lund, Sweden. alexander.ivanov@protista.se
Alexander E
AE
Ivanov
Nadezda
N
Solodukhina
Marie
M
Wahlgren
Lars
L
Nilsson
Alexander A
AA
Vikhrov
Maxim P
MP
Nikitin
Alexey V
AV
Orlov
Petr I
PI
Nikitin
Marina V
MV
Kuzimenkova
Vitali P
VP
Zubov
D000179:Acrylates / Q000737:chemistry; D000327:Adsorption; D001391:Azo Compounds / Q000737:chemistry; D001897:Boronic Acids / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D008968:Molecular Conformation; D009077:Mucins / Q000378:metabolism*; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism*; D013057:Spectrum Analysis; D014783:Viscosity
D000179:Acrylates; D001391:Azo Compounds; D001897:Boronic Acids; D009077:Mucins; C432250:N,N-dimethylacrylamide-co-4-phenylazophenylacrylate; D011108:Polymers; C010686:benzeneboronic acid