Effect of 3-hydroxyflavone on pig embryos produced by parthenogenesis or somatic cell nuclear transfer.
This study evaluated the effect of 3-hydroxyflavone (a flavonoid having hydroxyl group at 3 carbon position) on embryos using parthenogenetic activation (PA) and cloned pig embryos as model system. There was no evidence for embryo toxicity of 3-hydroxyflavone in a wide concentration range of 1-100 μM. On the contrary, 3-hydroxyflavone significantly improved the in vitro development and quality of PA embryos that was associated with the activation of ERK signaling molecules and reduction in Caspase 3 expression. Furthermore, 3-hydroxyflavone rescued the in vitro development and embryo quality of in vitro aged oocytes by inhibiting ROS activity and activating ERK signaling. The beneficial effects of 3-hydroxyflavone on PA embryos were consistent both in PVA- and BSA-containing embryo culture medium and in cloned embryos. These results suggest that, contrary to those of other structurally related flavonoid molecules, 3-hydroxyflavone may be useful as a therapeutic drug for improving the developmental potential of aged oocytes in assisted reproductive technologies.
Department of Animal Biotechnology, Bio-Organ Research Center/Animal Resources Research Center, Konkuk University, Seoul 143 701, South Korea.
Sang Jun
SJ
Uhm
Mukesh Kumar
MK
Gupta
Ziban Chandra
ZC
Das
Kyoung Tae
KT
Lim
Ji Hoon
JH
Yang
Hoon Taek
HT
Lee
D000818:Animals; D046149:Embryo Culture Techniques / Q000662:veterinary; D004622:Embryo, Mammalian / Q000187:drug effects*; D047108:Embryonic Development / Q000187:drug effects; D004789:Enzyme Activation / Q000187:drug effects; D005419:Flavonoids / Q000633:toxicity*; D053652:Nuclear Transfer Techniques; D009865:Oocytes / Q000187:drug effects / Q000254:growth & development; D010312:Parthenogenesis; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors; D013552:Swine / Q000196:embryology*
D005419:Flavonoids; D017382:Reactive Oxygen Species; C041477:3-hydroxyflavone