Identification, inheritance, and variation of microsatellite markers in the black scallop Mimachlamys varia.
Five polymorphic microsatellite loci were identified in the black scallop Mimachlamys varia after construction of a genomic library enriched for (GT)n. To examine the transmission pattern of microsatellite alleles, several families were created and genotypes scored for three loci. The expected Mendelian ratios were found in 12 of 14 segregations examined. Unexpected segregations may be explained by a genotyping error (allelic dropout), given that when a specific allele was treated as dominant, the phenotypic ratios conformed to Mendelian expectations. The five loci were also examined in two samples from the Spanish coast. The two localities displayed similar mean values for the number of alleles per locus (7.2-8.4), allelic richness (7.2-7.9), and observed (0.389-0.484) and expected heterozygosity (0.545-0.618). Significant Hardy-Weinberg deviations were observed at three loci, with heterozygote deficiency occurring in all cases. Global multilocus θ value and allele frequencies at one locus revealed significant differentiation between the two localities.
Departamento de Biología Celular y Molecular, Universidade da Coruña, A Zapateira s/n, A Coruña, Spain.
Alberto
A
Arias
Ruth
R
Freire
Juan Pablo
JP
De La Roche
Guillermo
G
Román
Josefina
J
Méndez
Ana
A
Insua
D000818:Animals; D001483:Base Sequence; D020090:Chromosome Segregation; D005787:Gene Frequency; D014644:Genetic Variation; D015698:Genomic Library; D005838:Genotype; D040941:Heredity; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D049897:Pectinidae / Q000235:genetics*; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA; D013030:Spain