Role of ribosomal protein S19-like plasma protein in blood coagulum resorption.
Western blot analyses and monocyte chemoattraction analyses of guinea pig plasma and serum indicated the presence of a plasma protein indistinguishable from ribosomal protein S19 and the cross-linked dimerization of it gaining monocyte chemotactic capacity in association with blood coagulation as in the case of human. When coagula preformed in vitro were intraperitoneally inserted into guinea pigs, they were rapidly covered by macrophages within 24h concomitant with an intra-coagulum macrophage infiltration. Differences were observed between the surface macrophages and the penetrating macrophages in ultrastructural, histochemical and immunohistochemical analyses. The inserted coagula were resorbed by day 7. When either anti-RP S19 antibodies or Gln137Asn-RP S19, a competitive inhibitor against RP S19, was premixed into the inserted coagulum, the attachment and penetration by macrophages decreased and the coagulum resorption retarded. These results indicate the role of the plasma RP S19-like molecule in coagulum resorption via macrophage recruitment.
Department of Molecular Pathology, Faculty of Life Science, Kumamoto University, Kumamoto, Japan.
Yoshihiko
Y
Ota
Jun
J
Chen
Masashi
M
Shin
Hiroshi
H
Nishiura
Kazutaka
K
Tokita
Masanori
M
Shinohara
Tetsuro
T
Yamamoto
D000818:Animals; D001777:Blood Coagulation / Q000502:physiology*; D002630:Chemotactic Factors / Q000097:blood*; D002634:Chemotaxis, Leukocyte; D019281:Dimerization; D006168:Guinea Pigs; D006801:Humans; D008264:Macrophages / Q000378:metabolism; D012269:Ribosomal Proteins / Q000037:antagonists & inhibitors / Q000097:blood* / Q000378:metabolism; D013927:Thrombosis / Q000378:metabolism*; D013997:Time Factors
D002630:Chemotactic Factors; D012269:Ribosomal Proteins; C040343:ribosomal protein S19