Rhizobium vallis sp. nov., isolated from nodules of three leguminous species.
Four bacterial strains isolated from root nodules of Phaseolus vulgaris, Mimosa pudica and Indigofera spicata plants grown in the Yunnan province of China were identified as a lineage within the genus Rhizobium according to the analysis of 16S rRNA gene sequences, sharing most similarity with Rhizobium lusitanum P1-7(T) (99.1 % sequence similarity) and Rhizobium rhizogenes IAM 13570(T) (99.0 %). These strains also formed a distinctive group from the reference strains for defined species of the genus Rhizobium in a polyphasic approach, including the phylogenetic analyses of the 16S rRNA gene and housekeeping genes (recA, atpD, glnII), DNA-DNA hybridization, BOX-PCR fingerprinting, phenotypic characterization, SDS-PAGE of whole-cell proteins, and cellular fatty acid profiles. All the data obtained in this study suggested that these strains represent a novel species of the genus Rhizobium, for which the name Rhizobium vallis sp. nov. is proposed. The DNA G+C content (mol%) of this species varied between 60.9 and 61.2 (T(m)). The type strain of R. vallis sp. nov. is CCBAU 65647(T) ( = LMG 25295(T) =HAMBI 3073(T)), which has a DNA G+C content of 60.9 mol% and forms effective nodules on Phaseolus vulgaris.
State Key Lab for Agrobiotechnology, College of Biological Sciences and Center for Biomass Engineering, China Agricultural University, Beijing 100193, PR China.
Fang
F
Wang
Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, 11340 México D. F., Mexico.|State Key Lab for Agrobiotechnology, College of Biological Sciences and Center for Biomass Engineering, China Agricultural University, Beijing 100193, PR China.
En Tao
ET
Wang
State Key Lab for Agrobiotechnology, College of Biological Sciences and Center for Biomass Engineering, China Agricultural University, Beijing 100193, PR China.
Li Juan
LJ
Wu
State Key Lab for Agrobiotechnology, College of Biological Sciences and Center for Biomass Engineering, China Agricultural University, Beijing 100193, PR China.
Xin Hua
XH
Sui
State Key Lab for Agrobiotechnology, College of Biological Sciences and Center for Biomass Engineering, China Agricultural University, Beijing 100193, PR China.
Ying
Y
Li
State Key Lab for Agrobiotechnology, College of Biological Sciences and Center for Biomass Engineering, China Agricultural University, Beijing 100193, PR China.
Wen Xin
WX
Chen
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D007887:Fabaceae / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012231:Rhizobium / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D053204:Root Nodules, Plant / Q000382:microbiology*
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S