Study on the decrease of renal D-amino acid oxidase activity in the rat after renal ischemia by chiral ligand exchange capillary electrophoresis.
D: -Amino acid oxidase (DAAO) in mammal kidney regulates the renal reactive oxygen species (ROS) levels directly and plays a leading role in the development of ROS-mediated renal pathologic damages based on its crucial role in the oxidative deamination of D: -amino acids and the consequent generation of H(2)O(2). Quantitative measurement of DAAO activity in the process of renal ischemia, which could help to understand the molecular mechanisms of this gripping acute renal disease, was conducted through the determination of chiral substrate by capillary electrophoresis (CE) in our study. In this study, a chiral ligand exchange CE method was explored with Zn(II)-L: -alaninamide complex as the chiral selector to investigate DAAO activity by determining the decreased concentration of the chiral substrate of DAAO-mediated enzymatic reaction. Then, the change of DAAO activity following 60-min acute renal ischemia in rats was observed with the proposed method. The study showed that the operation of renal ischemia resulted in a 45.49 ± 8.30% (n = 8) decrease in the DAAO-induced consumption of substrate, indicating a sharp decrease in renal DAAO activity following this acute renal injury. This phenomenon, with the possible reason of metabolic acidosis, could pave a new way for the study of oxidative stress in the development of renal ischemia injury.
Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, 100190, Beijing, China.
Haizhi
H
Zhang
Li
L
Qi
Yuqing
Y
Lin
Lanqun
L
Mao
Yi
Y
Chen
D000818:Animals; D003605:D-Amino-Acid Oxidase / Q000378:metabolism*; D004195:Disease Models, Animal; D019075:Electrophoresis, Capillary; D007511:Ischemia / Q000201:enzymology* / Q000378:metabolism; D007668:Kidney / Q000201:enzymology* / Q000378:metabolism / Q000473:pathology*; D008024:Ligands; D008297:Male; D008715:Methionine / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley
D008024:Ligands; D008715:Methionine; D003605:D-Amino-Acid Oxidase