[Study on cardiovascular diseases marker gene expression profile of familial aggregation hypertension].
OBJECTIVE
To explore the cardiovascular diseases marker gene expression profile of the familial aggregation hypertension patients,and to screen differentially expressed genes.


METHODS
The patients who had directly related family members for more than three generations suffering from hypertension were selected as experiment group, and healthy individuals as control group. Oligo GEArray gene chip technique was used to detect the expression of cardiovascular diseases marker gene in peripheral blood. The ratio of positive/negative standard value >2.0, or ≤0.5 and >0 was identified as differential gene.


RESULTS
Compared with control group, there were 10 up-regulated differential genes in experiment group, composing genes involved in lipid metabolism, immune response-related molecules, cell adhesion molecules, extracellular molecules and coagulation, including apolipoprotein E (ApoE), epithelial V-like antigen-1 (EVA-1), interferon-γ (IFN-γ), interleukin-1β (IL-1β), IL-8, integrin-β1 (ITGB-1), matrix metalloproteinase-9 (MMP-9), nuclear factor-ΚB (NF-ΚB), platelet endothelial cell adhesion molecule-1 (PECAM-1), selectin-P (SEL-P). There were 3 down-regulated genes, including coagulation factors-III (F-III), lectin-like oxidized low density lipoprotein receptor-1 (LOX-1), and serine protease inhibitor-1 (SERPINE-1).


CONCLUSIONS
This study suggested that familial aggregation hypertension related to a variety of gene markers of cardiovascular disease, especially elements concerning coagulation and extracellular protease inhibitor-related genes.
Department of Cardiology, First Hospital of Lanzhou University, Lanzhou 730000, Gansu, China. wangzhilu@medmail.com.cn
Zhi-lu
ZL
Wang
Zeng-ying
ZY
Wu
Xiao-fang
XF
Yang
Jian-wen
JW
Xiong
Shou-lan
SL
Shi
Ji-lin
JL
Ma
Po
P
Zhang
D015415:Biomarkers; D016022:Case-Control Studies; D006801:Humans; D006973:Hypertension / Q000235:genetics*; D007371:Interferon-gamma / Q000235:genetics; D053583:Interleukin-1beta / Q000235:genetics; D016209:Interleukin-8 / Q000235:genetics; D020780:Matrix Metalloproteinase 9 / Q000235:genetics; D016328:NF-kappa B / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000235:genetics; D059467:Transcriptome
D015415:Biomarkers; D053583:Interleukin-1beta; D016209:Interleukin-8; D016328:NF-kappa B; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D007371:Interferon-gamma; D020780:Matrix Metalloproteinase 9