[Study on purification of total flavonoids and saponins of Astragalus with macroporous resin].
OBJECTIVE
Through systematic study on many factors of influence of macroporous resin adsorption and separation, screening the best macroporous resin for purification of total flavonoids and saponins of Astragalus.


METHODS
The adsorption and desorption properties of total flavonoids and saponins on macroporous resins including D101, AB-8, XAD-1, HP-20, SP825, LSA-40, LX-18, XDA-5 and ADS-7 were compared. According to the results, XDA-5, D101 and AB-8 offered higher adsorption and desorption capacities and higher adsorption speed for total flavonoids and saponins than other resins. Column packed with XDA-5 resin was used to perform dynamic adsorption and desorption tests to optimize the separation process.


RESULTS
XDA-5 type macroporous resin owned optimum adsorption and elution parameters. The dynamic adsorption ratio of total flavonoids of Astragalus was 58.83 mg/mL after eluted with 4BV of distilled water and 4BV of 30%, 50%, 70% ethanol, respectively. Contents of total flavonoids and saponins of Astragalus were 28.75%, 44.38%, respectively.


CONCLUSIONS
It is a reliable and accurate method for development and industrial production of compound drug containing Astragalus.
School of Chinese materia medica, Guangzhou University of TCM, Guangzhou 510006, China. wzping_jshb@126.com
Zhi-Ping
ZP
Wang
Ying
Y
Gao
Wei-Min
WM
Li
Jie
J
Liu
D000327:Adsorption; D027883:Astragalus Plant / Q000737:chemistry*; D005419:Flavonoids / Q000032:analysis / Q000302:isolation & purification*; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D012117:Resins, Synthetic / Q000737:chemistry*; D012503:Saponins / Q000032:analysis / Q000302:isolation & purification*; D012997:Solvents / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D013678:Technology, Pharmaceutical / Q000379:methods*; D014867:Water / Q000737:chemistry
D005419:Flavonoids; D012117:Resins, Synthetic; D012503:Saponins; D012997:Solvents; D014867:Water