Study on the interaction between the inclusion complex of hematoxylin with β-cyclodextrin and DNA.
Ultraviolet-visible (UV-vis) spectra, fluorescence spectra, electrochemistry, and the thermodynamic method were used to discuss the interaction mode between the inclusion complex of hematoxylin with β-cyclodextrin and herring sperm DNA. On the condition of physiological pH, the result showed that hematoxylin and β-cyclodextrin formed an inclusion complex with binding ratio n(hematoxylin):n(β-cyclodextrin) = 1:1. The interaction mode between β-cyclodextrin-hematoxylin and DNA was a mixed binding, which contained intercalation and electrostatic mode. The binding ratio between β-cyclodextrin-hematoxylin and DNA was n(β-cyclodextrin -hematoxylin):n(DNA) = 2:1, binding constant was K(⊖)(298.15K) = 5.29 × 10⁴ L·mol⁻¹, and entropy worked as driven force in this action.
Department of Chemistry, Material Science and Engineering College, Southwest University of Science and Technology, Mianyang, Sichuan, P. R. China.
Dongling
D
Xu
Xingming
X
Wang
Dan
D
Fei
Lisheng
L
Ding
D000818:Animals; D004247:DNA / Q000737:chemistry*; D055664:Electrochemical Techniques; D005399:Fishes; D006416:Hematoxylin / Q000737:chemistry*; D007700:Kinetics; D008297:Male; D015394:Molecular Structure; D046912:Multiprotein Complexes / Q000138:chemical synthesis* / Q000737:chemistry; D011485:Protein Binding; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013094:Spermatozoa / Q000737:chemistry; D013816:Thermodynamics; D047392:beta-Cyclodextrins / Q000737:chemistry*
D046912:Multiprotein Complexes; D047392:beta-Cyclodextrins; D004247:DNA; C031215:betadex; D006416:Hematoxylin