The anti-hyperplasia of mammary gland effect of Thladiantha dubia root ethanol extract in rats reduced by estrogen and progestogen.
OBJECTIVE
Thladiantha dubia (Cucurbitaceae) has been used in Chinese manchu nationality folk medicine for treatment of various diseases, such as scrofula, carbuncle, lactation deficiency, etc. Its effects include softening hard masses, heat-clearing and detoxifying, reducing swelling, eliminating carbuncle, and promoting lactation. It has a history of use in the treatment of breast disorders and as an agent to increase lactation. The aim of the present study was to evaluate the anti-hyperplasia of mammary (HMG) effect of T. dubia root ethanol extract (TD-E) in estrogen combined with progestogen induced HMG rats.


METHODS
Forty virgin female Wistar rats were randomly divided into blank control, HMG model, Rupixiao Tablet (RPXT, positive control), and TD-E groups, 10 in each. Injection of estrogen and progestogen were given to establish rat models of HMG, RPXT and TD-E were given at the same time. Changes of nipple heights were measured; uterus and ovary index were calculated; sex hormone levels (LH, E(2), and P) in serum were estimated. Pathologic changes of MG in rats were also observed under light microscope.


CONCLUSIONS
TD-E could decrease the increased nipple height, reduce the numbers of MG lobules and relieve the pathologic changes of HMG in rats. It could also decrease E(2) and LH levels in serum compared with the control group, nipple height, uterus index, and the levels of E(2) and LH in the HMG model group increased significantly, the level of P decreased significantly. Compared with the HMG model group, nipple height, uterus index, and the levels of E(2) and LH in the TD-E group decreased, and no obvious effect on P content. The histopathological analysis suggested that TD-E obviously alleviated the degree of HMG. Those results suggest TD-E has protective and therapeutic effects on HMG rats induced by estrogen and progestogen.
Jilin Provincial Academy of Chinese Medicine Sciences, Changchun 130021, China.
Lishu
L
Wang
Daqing
D
Zhao
Lin
L
Di
Dongyan
D
Cheng
Xuefeng
X
Zhou
Xianwen
X
Yang
Yonghong
Y
Liu
D000818:Animals; D019662:Cucurbitaceae / Q000737:chemistry*; D004967:Estrogens / Q000494:pharmacology*; D000431:Ethanol / Q000737:chemistry; D005260:Female; D006965:Hyperplasia / Q000517:prevention & control*; D007928:Lethal Dose 50; D008321:Mammary Glands, Animal / Q000187:drug effects* / Q000473:pathology; D010936:Plant Extracts / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry*; D011374:Progesterone / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
D004967:Estrogens; D010936:Plant Extracts; D000431:Ethanol; D011374:Progesterone