[Observation on cytoskeleton and root tip growth of Arabidopsis thaliana by two-photon laser scanning microscopy].
Cytoskeleton plays an important role in plant cells and their movements, developments, and so on. With MS culture medium, the present paper cultured GFP-fused Arabidopsis thaliana in asepsis condition, which completed its whole lifecycle in the lab, starting from germination, going through plant growth, blossom, and ending at fructification. During this process, the authors used two-photon laser scanning microscopy (TPLSM) which was suitable for 4D observation on large, thick, live samples to observe cytoskeleton in seed, root tip, vessel and root hair of the Arabidopsis thaliana. Dynamic root tip growth as well as the growing speed was also observed. Applying IAA to the Arabidopsis thaliana, the authors found that the growing speed of root tip was enhanced by about 3.8 times.
Key Laboratory for Atomic and Molecular Nanosciences of Ministry of Education, Department of Physics, Tsinghua University, Beijing 100084, China. feofio@gmail.com
Peng-fei
PF
Wang
Dan-ying
DY
Lin
Wan-yun
WY
Ma
D017360:Arabidopsis / Q000166:cytology*; D003599:Cytoskeleton / Q000502:physiology*; D018519:Meristem / Q000254:growth & development; D018613:Microscopy, Confocal; D018517:Plant Roots / Q000254:growth & development*