Identification of oral bacteria by 16S rRNA gene analysis in elderly persons requiring nursing care.
After incubation of saliva from 58 semi-bedridden elderly persons, the cultures were identified based on the 16S rRNA gene base sequence to compare the identification by the conventional culture method. As a result, the 16S rRNA gene base sequence of 198 strains identified by the culture method showed 98.5% or more homology in some of the Human Oral Microbiome database, and the identification of bacterial species and genus was possible. When an organism identified by the 16S rRNA gene sequencing method was compared with that by the culture method, the concordance rates were 54.5% at the genus level and 35.9% at the species level. Streptococcus mitis strains most frequently isolated from saliva that were identified by the culture method were identified as the same species by the 16S rRNA gene sequencing method (32/35), and all the 11 Streptococcus salivarius strains identified by the culture method were identified as the same species by the 16S rRNA gene sequencing method. All the strains identified as Streptococcus anginosus group by the culture method and 8 of the 9 strains identified as Prevotella species by the culture method were identified as the same group and genus by the 16S rRNA gene sequencing method. When an oral microbial flora test with saliva samples from elderly persons is performed, the 16S rRNA gene sequence identification enables us to identify major indigenous bacteria and pathogenic bacteria and is considered useful as a means of supplementing the conventional culture method.
Department of Oral Surgery, Tokai University, School of Medicine, Isehara, Kanagawa, Japan.
Hirotaka
H
Kurabayashi
Akihiro
A
Kaneko
Ryo
R
Sekiya
Kazunari
K
Karakida
Masashi
M
Sasaki
Noriko
N
Nakatogawa
Takayuki
T
Aoki
Yoshihide
Y
Ota
Haruo
H
Sakamoto
D000368:Aged; D000369:Aged, 80 and over; D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000737:chemistry* / Q000235:genetics; D005260:Female; D006801:Humans; D008297:Male; D009735:Nursing Homes; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000737:chemistry*; D012463:Saliva / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013291:Streptococcus
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S