Assessment of angiogenesis by CD105 and nestin expression in peritumor tissue of glioblastoma.
Angiogenesis in the peritumor tissue of glioblastoma (GBM) is still an open field of research. This study investigates neovascularization in the tumor surrounding areas by examining CD105 and nestin expression along with microvessel density (MVD) with the aim of establishing their possible prognostic significance. Angiogenesis was also confirmed by investigating, in vessel walls, the presence of pericytes, which are multipotent stem cells, expressing α-smooth muscle actin (α-SMA). In our study, including 40 GBM patients, tissue samples were obtained from tumors (first area) and white matter at a distance <1 cm (second area) and between 1 and 3.5 cm (third area) from the tumor margin. CD105 and nestin were detected by immunohistochemistry in hyperplastic endothelium of GBM and peritumor tissue, and occasionally coexpressed or colocalized. Pericytes encircling hyperplastic endothelium were evident in all three areas. Univariate analysis revealed that patients with a CD105-MVD value ≥8 in the third area have a significantly shorter survival time and Cox analysis indicated an about 3.5-fold increase in death risk in the same patients. These results demonstrate that a tumor neoangiogenesis occurs in GBM peritumor tissue with intimate involvement of pericytes. CD105-MVD in the area located at a greater distance from the tumor margin carries prognostic significance.
Institute of Histology and Embryology, Faculty of Medicine, Catholic University of the Sacred Heart, Largo Francesco Vito 1, I-00168 Rome, Italy. gigliola@rm.unicatt.it
Gigliola
G
Sica
Gina
G
Lama
Carmelo
C
Anile
Maria Concetta
MC
Geloso
Giuseppe
G
La Torre
Pasquale
P
De Bonis
Giulio
G
Maira
Libero
L
Lauriola
Meena
M
Jhanwar-Uniyal
Annunziato
A
Mangiola
D000199:Actins / Q000378:metabolism; D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000096:biosynthesis*; D000071063:Endoglin; D005260:Female; D005909:Glioblastoma / Q000098:blood supply* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D007381:Intermediate Filament Proteins / Q000096:biosynthesis*; D008297:Male; D008875:Middle Aged; D014411:Neoplastic Stem Cells / Q000378:metabolism / Q000473:pathology; D009389:Neovascularization, Pathologic / Q000378:metabolism / Q000473:pathology; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D064231:Nestin; D011956:Receptors, Cell Surface / Q000096:biosynthesis*; D015173:Supratentorial Neoplasms / Q000098:blood supply* / Q000378:metabolism / Q000473:pathology
C541116:ACTA2 protein, human; D000199:Actins; D015703:Antigens, CD; C064206:ENG protein, human; D000071063:Endoglin; D007381:Intermediate Filament Proteins; C577484:NES protein, human; D009419:Nerve Tissue Proteins; D064231:Nestin; D011956:Receptors, Cell Surface