MicroRNA expression profile of colon cancer stem-like cells in HT29 adenocarcinoma cell line.
Increasing evidence has suggested cancer stem cells (CSCs) are considered to be responsible for cancer formation, recurrence, and metastasis. Recently, many studies have also revealed that microRNAs (miRNAs) strongly implicate in regulating self renewal and tumorigenicity of CSCs in human cancers. However, with respect to colon cancer, the role of miRNAs in stemness maintenance and tumorigenicity of CSCs still remains to be unknown. In the present study, we isolated a population of colon CSCs expressing a CD133 surface phenotype from human HT29 colonic adenocarcinoma cell line by Flow Cytometry Cell Sorting. The CD133(+) cells possess a greater tumor sphere-forming efficiency in vitro and higher tumorigenic potential in vivo. Furthermore, the CD133(+) cells are endowed with stem/progenitor cells-like property including expression of "stemness" genes involved in Wnt2, BMI1, Oct3/4, Notch1, C-myc and other genes as well as self-renewal and differentiation capacity. Moreover, we investigated the miRNA expression profile of colon CSCs using miRNA array. Consequently, we identified a colon CSCs miRNA signature comprising 11 overexpressed and 8 underexpressed miRNAs, such as miR-429, miR-155, and miR-320d, some of which may be involved in regulation of stem cell differentiation. Our results suggest that miRNAs might play important roles in stemness maintenance of colon CSCs, and analysis of specific miRNA expression signatures may contribute to potential cancer therapy.
Department of Oncology, Renji Hospital, Shanghai Jiao Tong University, School of Medicine, Dongfang Road 1630, Shanghai 200127, China.
Huanle
H
Zhang
Weihua
W
Li
Feifei
F
Nan
Fang
F
Ren
Hongxia
H
Wang
Yingchun
Y
Xu
Fengchun
F
Zhang
D000230:Adenocarcinoma / Q000378:metabolism* / Q000473:pathology; D015703:Antigens, CD / Q000032:analysis; D015214:Antigens, Differentiation, Myelomonocytic / Q000032:analysis; D002469:Cell Separation; D002471:Cell Transformation, Neoplastic / Q000378:metabolism* / Q000473:pathology; D003110:Colonic Neoplasms / Q000378:metabolism* / Q000473:pathology; D019073:HT29 Cells; D006801:Humans; D035683:MicroRNAs / Q000096:biosynthesis*; D014411:Neoplastic Stem Cells / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology; D063268:Sialic Acid Binding Ig-like Lectin 3
D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; C571163:CD33 protein, human; D035683:MicroRNAs; D063268:Sialic Acid Binding Ig-like Lectin 3