Enhancement of the immunogenicity of synthetic carbohydrate vaccines by chemical modifications of STn antigen.
The abnormal glycans expressed on the surface of tumor cells, known as tumor-associated carbohydrate antigens, increase the chance to develop carbohydrate-based anticancer vaccines. However, carbohydrate antigens pose certain difficulties, and the major drawback is their weak immunogenicity. To tackle this problem, numerous structurally modified STn antigens were designed and synthesized in this work. These synthetic antigens were screened in vitro by using competitive ELISA method, and the antigens with positive response were conjugated to the protein carrier for vaccination. The vaccination results on mice showed that some fluorine-containing modifications on the STn antigen can significantly increase the anti-STn IgG titers and improve the ratios of anti-STn IgG/IgM. The antisera can recognize the tumor cells expressing the native STn antigen.
State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, China.
Fan
F
Yang
Xiu-Jing
XJ
Zheng
Chang-Xin
CX
Huo
Yue
Y
Wang
Ye
Y
Zhang
Xin-Shan
XS
Ye
D000818:Animals; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000737:chemistry* / Q000276:immunology*; D019496:Cancer Vaccines / Q000138:chemical synthesis / Q000737:chemistry / Q000276:immunology*; D002236:Carbohydrate Conformation; D002241:Carbohydrates / Q000138:chemical synthesis / Q000737:chemistry / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007074:Immunoglobulin G / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D013237:Stereoisomerism; D014611:Vaccination; D014614:Vaccines, Synthetic / Q000737:chemistry / Q000276:immunology*
D015295:Antigens, Tumor-Associated, Carbohydrate; D019496:Cancer Vaccines; D002241:Carbohydrates; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D014614:Vaccines, Synthetic; C055491:sialosyl-Tn antigen