Comparative proteomic analysis of biofilm and planktonic cells of Neisseria meningitidis.
Neisseria meningitidis is a commensal of the human nasopharynx occasionally causing invasive disease. In vitro biofilms have been employed to model meningococcal carriage. A proteomic analysis of meningococcal biofilms was conducted and metabolic changes related to oxygen and nutrient limitation and upregulation of proteins involved in ROS defense were observed. The upregulated MntC which protects against ROS was shown to be required for meningococcal biofilm formation, but not for planktonic growth. ROS-induced proteomic changes might train the biofilm to cope with immune effectors.
Institute for Hygiene and Microbiology, University of Würzburg, Würzburg, Germany.
Tessa
T
van Alen
Heike
H
Claus
René P
RP
Zahedi
Janos
J
Groh
Heinrich
H
Blazyca
Martin
M
Lappann
Albert
A
Sickmann
Ulrich
U
Vogel
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D018441:Biofilms / Q000254:growth & development; D009154:Mutation; D009345:Neisseria meningitidis / Q000254:growth & development / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism
D001426:Bacterial Proteins; D020543:Proteome; D017382:Reactive Oxygen Species