[Determination of protopine in Corydalis racemose by HPLC].
OBJECTIVE
To develop a HPLC method for determining the content of protopine in Corydalis racemose.


METHODS
Analysis was performed on a Gemini C18 column (4.6 mm x 250 mm, 5 microm) eluted with acetonitrile-water containing 0.8% triethylamine and 3% acetic acid acetum (20:80) as the mobile phase. The flow rate was 1.0 mL x min(-1). The detection wavelength was 289 nm.


RESULTS
The average content of protopine in Herb of Racemose Corydalis was 0.905%. The calibration curve of protopine was linear between 0.124-1.36 microg (r = 0.9999). The average recovery was 98.49% with RSD 1.9%.


CONCLUSIONS
This method is simple, reproducible and can be used to determine the content of protopine in C. racemose.
Fujian Academy of Integrative Medicine, Fuzhou 350108, China.
Xiazhi
X
Jiang
Jinxia
J
Ye
Jianwei
J
Zeng
Xiuhong
X
Zou
Jinzhong
J
Wu
D053119:Benzophenanthridines / Q000032:analysis*; D001600:Berberine Alkaloids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D031311:Corydalis / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000032:analysis*
D053119:Benzophenanthridines; D001600:Berberine Alkaloids; D004365:Drugs, Chinese Herbal; C009093:protopine