Investigation of effects of newly synthesized Pt(II) complex against human serum albumin and leukemia cell line of K562.
The biological evaluation of a new synthesized Pt(II)-complex, 2,2'-bipyridin Butylglycinato Pt(II) nitrate, an anti-tumor component, was studied at different temperatures by fluorescence and far UV circular dichroism (CD) spectroscopic methods. Human serum albumin (HSA) and human tumor cell line K562 were as targets. The Pt(II)-complex has a strong ability to quench the intrinsic fluorescence of HSA. Binding and thermodynamic parameters of the interaction were calculated by fluorescence quenching method. Far-UV-CD results showed that Pt(II)-complex induced increasing in content of α helical structure of the protein and stabilized it. The 50% cytotoxic concentration (Cc(50)) of complex was determined using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay at different incubation times. Also, fluorescence staining with DAPI (4,6-diamidino-2-phenylindole) revealed some typical nuclear changes, which are characteristic of apoptosis. Above results suggest that Pt (II) complex is a promising anti-proliferative agent and should execute its biological effects by inducing apoptosis.
Institute of Biochemistry and Biophysics, University of Tehran, Iran. divsalar@ibb.ut.ac.ir
Adeleh
A
Divsalar
Ali A
AA
Saboury
Leila
L
Ahadi
Elham
E
Zemanatiyar
Hassan
H
Mansouri-Torshizi
D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000633:toxicity*; D017209:Apoptosis; D002942:Circular Dichroism; D056831:Coordination Complexes / Q000138:chemical synthesis / Q000737:chemistry / Q000633:toxicity; D006801:Humans; D020014:K562 Cells; D009944:Organoplatinum Compounds / Q000138:chemical synthesis / Q000737:chemistry / Q000633:toxicity*; D010165:Palladium / Q000737:chemistry; D011485:Protein Binding; D012709:Serum Albumin / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013816:Thermodynamics
C556663:2,2'-bipyridin butylglycinato platinum(II); D000970:Antineoplastic Agents; D056831:Coordination Complexes; D009944:Organoplatinum Compounds; D012709:Serum Albumin; D010165:Palladium