Highly branched polymers with polymyxin end groups responsive to Pseudomonas aeruginosa.
Polymyxin peptide conjugated to the end groups of highly branched poly(N-isopropyl acrylamide) was shown to bind to a Gram negative bacterium, Pseudomonas aeruginosa . The nonbound polymer had a lower critical solution temperature (LCST) above 60 °C. However, binding caused aggregation, which was disrupted on cooling of the bacteria and polymer mixture. The data indicate that polymer binding of bacteria occurred by interaction of the end groups with lipopolysaccharide and that the binding decreased the LCST to below 37 °C. Cooling then progressed the polymer/bacteria aggregate through a bound LCST into an open polymer coil conformation that was not adhesive to P. aeruginosa .
Polymer and Biomaterials Chemistry Laboratories, Department of Chemistry, University of Sheffield, Sheffield, South Yorkshire, S3 7HF United Kingdom.
Prodip
P
Sarker
Joanna
J
Shepherd
Kathryn
K
Swindells
Ian
I
Douglas
Sheila
S
MacNeil
Linda
L
Swanson
Stephen
S
Rimmer
D000178:Acrylamides / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D000180:Acrylic Resins; D000900:Anti-Bacterial Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000737:chemistry / Q000378:metabolism; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D011112:Polymyxin B / Q000737:chemistry / Q000494:pharmacology; D011550:Pseudomonas aeruginosa / Q000737:chemistry / Q000254:growth & development* / Q000378:metabolism
D000178:Acrylamides; D000180:Acrylic Resins; D000900:Anti-Bacterial Agents; D008070:Lipopolysaccharides; D011108:Polymers; C052970:poly-N-isopropylacrylamide; D011112:Polymyxin B