Increased intracellular iron and mineralization of cultured hFOB 1.19 cells following hepcidin activation through ferroportin-1.
OBJECTIVE
To address whether hepcidin functions in bone metabolism.


METHODS
This study was carried out in the Laboratory of Radiation Medicine and Public Health of Soochow University, and the Laboratory of the Second Affiliated Hospital of Soochow University, Suzhou, China, from September 2009 to July 2010. The positive expression of ferroportin-1 (Fpn-1) was detected by reverse transcriptase-polymerase chain reaction. After the treatment with distilled water (control group) and hepcidin (25noml/L, 50noml/L, 100noml/L), the fluorescence intensity related to intracellular iron concentration of a human fetal osteoblast cell line (hFOB 1.19) was measured by a confocal laser scanning microscope. A 3-(4,5- dimethylthiazol-2-yl) -2-5-diphenyltetrazolium bromide assay, and Von Kossa staining was performed to evaluate cell proliferation and mineralization in cultured hFOB 1.19 cells.


RESULTS
This study revealed a high level expression of Fpn-1 in hFOB 1.19. On the basis of which, it was found that 25noml/L, 50noml/L, 100noml/L hepcidin could promote the fluorescence intensity related to intracellular iron concentration and mineralization in hFOB 1.19 in a dose-dependent manner (p<0.05), but hepcidin had no effect on FOB 1.19 proliferation (p>0.05).


CONCLUSIONS
The hepcidin-ferroportin signal pathway may function in the osteoblast cell line of hFOB 1.19 cells. It is also suggested that cross-talk between iron and calcium homeostasis may play a role in bone metabolism in responding to hepcidin activation.
Department of Orthopedics, The Second Affiliated Hospital of SooChow University, Suzhou, China.
Peng
P
Zhang
You-jia
YJ
Xu
Dong-Yang
DY
Zhao
Yong
Y
Ma
Li
L
Xiao
Yu-Shuang
YS
Feng
Ben-Cai
BC
Du
Zhong-Ming
ZM
Qian
Kai
K
Li
D023181:Antimicrobial Cationic Peptides / Q000378:metabolism*; D001483:Base Sequence; D027682:Cation Transport Proteins / Q000378:metabolism*; D002460:Cell Line; D049109:Cell Proliferation; D017931:DNA Primers; D064451:Hepcidins; D006801:Humans; D007501:Iron / Q000378:metabolism*; D018613:Microscopy, Confocal; D008903:Minerals / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000098742:Ferroportin
D023181:Antimicrobial Cationic Peptides; D027682:Cation Transport Proteins; D017931:DNA Primers; C578702:HAMP protein, human; D064451:Hepcidins; D008903:Minerals; D000098742:Ferroportin; D007501:Iron