Changes in gene expression following cardiac pacing-induced delayed cardioprotection in the canine heart.
The aim of the present study was to identify gene expression changes in the rapid cardiac pacing-induced delayed antiarrhythmic protection in the canine, using cDNA microarrays and quantitative real-time PCR (QRT -PCR) techniques. In all dogs under light pentobarbitone anaesthesia, a pacing electrode was introduced into the right ventricle, and then the animals were divided into three groups: (1) sham-operated and sham-paced group (SP, n = 3) (2) ischaemic control group (IC; n = 3); these were without cardiac pacing and subjected only to a 25 min occlusion of the left anterior descending coronary artery (LAD), and (3) paced group (PC, n = 3); these animals were paced at a rate of 220-240 beats min-1 24 h prior to ischaemia. With cDNA chip 23 genes were found with altered expression in response to rapid cardiac pacing and 10 genes in the IC group when compared to SP dogs. These genes encode transcription factors (MEF2); members of signaling pathways (TGFβ2, PDE4D9), hormone related proteins (e.g. vasopressin V1 and V2 receptors). RT-QPCR was used either to confirm the results of the microarray analysis and also to study 46 genes which are already known to have a role in the late phase of PC. By this method 17 genes were up-regulated and 6 genes down-regulated in the IC group; their expression ratios changed either to the opposite or showed no alteration after cardiac pacing. This study would add some new information about those transcriptional changes that are involved in the delayed phase of cardiac protection.
Department of Pharmacology and Pharmacotherapy, Faculty of Medicine, University of Szeged, Szeged, Hungary.
Mária
M
Kovács
Rita
R
Papp
Z
Z
Varga-Orvos
Dalma
D
Ménesi
L G
LG
Puskás
Agnes
A
Végh
D000758:Anesthesia; D000818:Animals; D002304:Cardiac Pacing, Artificial / Q000379:methods*; D003331:Coronary Vessels / Q000473:pathology; D018076:DNA, Complementary / Q000378:metabolism; D004285:Dogs; D015536:Down-Regulation; D005260:Female; D020869:Gene Expression Profiling; D006321:Heart / Q000502:physiology; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D010424:Pentobarbital / Q000494:pharmacology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation
D018076:DNA, Complementary; D010424:Pentobarbital