Important role of catalase in the production of β-carotene by recombinant Saccharomyces cerevisiae under H2O2 stress.
The effect of H(2)O(2) supplement on cell growth and β-carotene productions in recombinant Saccharomyces cerevisiae CFW-01 and CFW-01 ctt1 deficiency in cytosolic catalase were investigated in shaking flasks. The results showed that supplement of H(2)O(2) (0.5 and 1.0 mM) can significantly stimulate the β-carotene production. However, β-carotene levels of CFW-01 ctt1Δ under 0.5 and 1 mM H(2)O(2) were 16.7 and 36.7% lower than those of CFW-01, respectively. Although lacking cytosolic catalase, no significant differences in cell growth were observed between CFW-01 ctt1Δ and CFW-01 under the same level of H(2)O(2) stress. These results suggest that β-carotene can act as an antioxidant to protect the recombinant yeast from H(2)O(2) oxidative damage in the absence of cytosolic catalase. However, catalase still plays an important role in the production of β-carotene under H(2)O(2) stress. If catalase can not timely decompose H(2)O(2), the free radicals such as OH· derived from H(2)O(2) can result in decrease of β-carotene concentration. Therefore, in the production of β-carotene by H(2)O(2) stress, not only the level of oxidative stress, but also the activities of catalase in cells should be considered.
Center for Viticulture and Enology, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, People's Republic of China.
Guo-liang
GL
Yan
Heng-yu
HY
Liang
Zhi-qun
ZQ
Wang
Xiao-fan
XF
Yang
Dan
D
Liu
Jin-fu
JF
Liu
Chang-qing
CQ
Duan
D000975:Antioxidants / Q000378:metabolism; D002374:Catalase / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000633:toxicity*; D030781:Organisms, Genetically Modified; D018384:Oxidative Stress; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D013312:Stress, Physiological; D019207:beta Carotene / Q000378:metabolism*
D000975:Antioxidants; D019207:beta Carotene; D006861:Hydrogen Peroxide; D002374:Catalase