Isolation and characterization of oil degrading bacteria from oil contaminated soils of Vellore district, Tamil Nadu, India.
Oil contaminated soils were collected from oil grinding mill, automobile service stations, and restaurant waste dumping sites of Vellore district for the isolation of oil degrading bacteria. Out of 78 Colony Forming Units, only 16 were found to be potent bacteria. 8 biggest clear zone forming bacteria on trybutyrin plates were studied for their colony morphology and the most potential oil degrader bacteria was then chosen for microbial and biochemical assay. The study showed the most prospective bacteria were Bacillus subtilis with the dimension of 3.45 microm x 0.2 microm. This "microbial oil-destroyer" produces extracellular lipase which utilizes oil, and hence it can be used for the self remediation of lipid contaminated soils and water bodies.
Division of Environmental Biotechnology, School of Biotechnology, Chemical and Biomedical Engineering, Vellore Institute of Technology University, Vellore, Tamil Nadu-632014. aprdbt@gmail.com
Anand Prem
AP
Rajan
D001419:Bacteria / Q000302:isolation & purification* / Q000378:metabolism*; D001673:Biodegradation, Environmental; D005648:Fuel Oils / Q000032:analysis*; D007194:India; D012498:Sanitary Engineering; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*
D005648:Fuel Oils; D012989:Soil Pollutants