A tissue-specific, activation-inducible, lentiviral vector regulated by human CD40L proximal promoter sequences.
The application of new protocols for gene therapy against monogenic diseases requires the development of safer therapeutic vectors, particularly in the case of diseases in which expression of the mutated gene is subject to fine regulation, as it is with CD40L (CD154). CD40L, the gene mutated in the X-linked hyper-immunoglobulin M syndrome (HIGM1), is tightly regulated to allow surface expression of its product only on T cells stimulated by antigen encounter. Previous studies in an HIGM1 animal model showed that transduction of progenitor cells corrected the syndrome but caused a thymic lymphoproliferative disease because of the unregulated expression of the transgene by constitutive vectors. To develop a tissue-specific, activation-inducible, lentiviral vector (LV) for gene therapy to counter HIGM1, we have constructed two self-inactivating LVs, pCD40L-eGFP and pCD40L-CD40L, regulated by a 1.3 kb fragment of the human CD40L proximal promoter. The expression of pCD40L-eGFP LV is restricted to cells in which mRNA transcripts of the endogenous CD40L gene can be detected. Moreover, the expression of the reporter gene in primary T lymphocytes depends on the activation state of the cells. Remarkably, primary HIGM1 lymphocytes transduced with pCD40L-CD40L LV expressed CD40L only after T-cell stimulation. Therefore, the CD40L-promoter-driven vectors are able to achieve a near-physiological expression pattern that follows very closely that of the endogenous CD40L gene.
Institute of Biopathology and Regenerative Medicine, Center for Biomedical Research, University of Granada, Health-Science Technology Park, Armilla, Granada, Spain.
Z
Z
Romero
S
S
Torres
M
M
Cobo
P
P
Muñoz
J D
JD
Unciti
F
F
Martín
I J
IJ
Molina
D023201:CD40 Ligand / Q000235:genetics*; D002460:Cell Line; D015316:Genetic Therapy; D005822:Genetic Vectors; D006801:Humans; D053307:Hyper-IgM Immunodeficiency Syndrome, Type 1 / Q000235:genetics / Q000628:therapy; D016086:Lentivirus / Q000235:genetics*; D008213:Lymphocyte Activation; D009928:Organ Specificity; D011401:Promoter Regions, Genetic; D013601:T-Lymphocytes / Q000378:metabolism; D014161:Transduction, Genetic
D023201:CD40 Ligand