Enhancement of Zta-activated lytic transcription of Epstein-Barr virus by Ku80.
Zta, encoded by the BZLF1 gene of Epstein-Barr virus (EBV), is a transcription factor that is expressed during the immediate-early stage of the lytic cycle. The expression of Zta is crucial to viral lytic development. Earlier studies showed that Ku80 is a binding partner of Zta in ZKO-293 cells and is co-purified with Zta. This study verifies the interaction between Ku80 and Zta by using glutathione S-transferase-pull-down and co-immunoprecipitation assays, and also by indirect immunofluorescence analysis. This investigation also reveals that Ku80 binds to Zta on Zta-response elements in the BHLF1 promoter, enhancing the promoter activity. This study also reveals that the interaction between Zta and Ku80 involves the C-terminal region of Zta and the 425 aa N-terminal region of Ku80. The interaction between these two proteins and the enhancement of transcription that is activated by Zta suggest that Ku80 is important to EBV lytic development.
Department of Biochemical Science and Technology, College of Life Science, National Taiwan University, Taipei 106, Taiwan, ROC.
Chien-Chang
CC
Chen
Ya-Chun
YC
Yang
Wen-Hung
WH
Wang
Chien-Sin
CS
Chen
Li-Kwan
LK
Chang
D034961:Antigens, Nuclear / Q000378:metabolism*; D002460:Cell Line; D004279:DNA, Viral / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism*; D019084:Fluorescent Antibody Technique, Indirect; D015967:Gene Expression Regulation, Viral; D004854:Herpesvirus 4, Human / Q000502:physiology*; D054884:Host-Pathogen Interactions; D006801:Humans; D047468:Immunoprecipitation; D000072200:Ku Autoantigen; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D025941:Protein Interaction Mapping; D015534:Trans-Activators / Q000378:metabolism*; D014158:Transcription, Genetic
D034961:Antigens, Nuclear; C063676:BZLF1 protein, Herpesvirus 4, Human; D004279:DNA, Viral; D004268:DNA-Binding Proteins; D015534:Trans-Activators; C000606509:Xrcc6 protein, human; D000072200:Ku Autoantigen