[Determination of ilexside II in leaves of Ilex cornuta by HPLC-ELSD].
OBJECTIVE
To develop a HPLC-ELSD method for determination of ilexside II in leaves of Ilex cornuta.


METHODS
The separation was performed on a Waters Syemmetry Shield RP18 column (4.6 mm x 250 mm, 5 microm) and methanol-water was used as mobile phase in gradient elution. The flow was 1.0 mL x min(-1), and the temperature was set at 35 degrees C.


RESULTS
The calibration curve showed good linearity in the test range (R2 = 0.9997). And the mean recovery was 101.3%, RSD was 2.0% (n=6).


CONCLUSIONS
The developed HPLC-ELSD method was accurate and reproducible, and can be used for the determination of ilexside II in leaves of Ilex cornuta.
State Key Laboratory of Natural and Biomimetic Drugs, School of Phannrmaceutical Sciences, Peking University, Beijing 100191, China.
Zhimrong
Z
Yao
Jun
J
Li
Mingbo
M
Zhao
Li
L
Wang
Pengfei
P
Tu
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D006027:Glycosides / Q000032:analysis*; D030017:Ilex / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D014315:Triterpenes / Q000032:analysis*
D004365:Drugs, Chinese Herbal; D006027:Glycosides; D014315:Triterpenes; C554779:ilexside II