A versatile approach to high-throughput microarrays using thiol-ene chemistry.
Microarray technology has become extremely useful in expediting the investigation of large libraries of materials in a variety of biomedical applications, such as in DNA chips, protein and cellular microarrays. In the development of cellular microarrays, traditional high-throughput printing strategies on stiff, glass substrates and non-covalent attachment methods are limiting. We have developed a facile strategy to fabricate multifunctional high-throughput microarrays embedded at the surface of a hydrogel substrate using thiol-ene chemistry. This user-friendly method provides a platform for the immobilization of a combination of bioactive and diagnostic molecules, such as peptides and dyes, at the surface of poly(ethylene glycol)-based hydrogels. The robust and orthogonal nature of thiol-ene chemistry allows for a range of covalent attachment strategies in a fast and reliable manner, and two complementary strategies for the attachment of active molecules are demonstrated.
Materials Research Laboratory, University of California, Santa Barbara, California 93105, USA.
Nalini
N
Gupta
Brian F
BF
Lin
Luis M
LM
Campos
Michael D
MD
Dimitriou
Sherry T
ST
Hikita
Neil D
ND
Treat
Matthew V
MV
Tirrell
Dennis O
DO
Clegg
Edward J
EJ
Kramer
Craig J
CJ
Hawker
D000475:Alkenes / Q000737:chemistry*; D000595:Amino Acid Sequence; D000818:Animals; D040761:Biomimetic Materials / Q000737:chemistry; D002448:Cell Adhesion; D020100:Hydrogels / Q000737:chemistry; D051379:Mice; D046228:Microarray Analysis; D055616:Microtechnology / Q000379:methods*; D041681:NIH 3T3 Cells; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D011327:Printing; D013438:Sulfhydryl Compounds / Q000737:chemistry*
D000475:Alkenes; D020100:Hydrogels; D010455:Peptides; D013438:Sulfhydryl Compounds