[Effects of local hyperthermia on maturation of Langerhans cells in HPV-infected skin].
OBJECTIVE
To investigate the possible mechanism of local hyperthermia in the treatment of warts through detecting the differences in CD1a/CD83 of Langerhans cells (LCs) in émigrés from HPV-infected skin, as compared to normal skin.


METHODS
Confocal microscopy were performed on Condyloma Accuminatum (CA)and normal skin; Freshly taken biopsies of CA and normal skin were subjected to surface heating at 37 degrees C, 42 degrees C and 45 degrees C respectively, for 30 mins. Flow cytometry was used to determine the CD1a/ CD83 changes of LCs in émigrés from CA and normal skin.


RESULTS
By confocal microscopic observation, there were practically no CD1a+ LCs that expressed CD83 in the epidermis of both normal skin and CA. The proportions of CD1a+/CD83 LCs were significantly increased with increased temperatures in émigrés from both normal skin and CA. At each given temperature, the numbers of LCs in émigrés from CA were greater than those from normal skin.


CONCLUSIONS
Local hyperthermia can promote migration and maturation of LCs in HPV-infected skin and accordingly stimulate the immune system to treat warts.
Department of Dermatology, No. 1 Hospital of China Medical University, Shenyang 110001, China.
Xiao-Dong
XD
Li
Xing-Hua
XH
Gao
Yu-Xiao
YX
Hong
Yu-Bo
YB
Liu
Li-Li
LL
Zhu
Ya-Kun
YK
Wang
Xiao-Qin
XQ
Wang
Wei
W
Huo
Li
L
Zhang
Hong-Duo
HD
Chen
D000328:Adult; D002465:Cell Movement / Q000276:immunology; D005260:Female; D006801:Humans; D006979:Hyperthermia, Induced; D066298:In Vitro Techniques; D007801:Langerhans Cells / Q000166:cytology* / Q000276:immunology; D027383:Papillomaviridae / Q000276:immunology* / Q000472:pathogenicity; D012867:Skin / Q000276:immunology* / Q000821:virology*; D055815:Young Adult