Antisense effect of pyrrolidine-based oxy-peptide nucleic acids in Escherichia coli.
To investigate the antisense effect of a pyrrolidine-based oxy-peptide nucleic acid (POPNA), we carried out the LacZ reporter assay using a 12-mer trans-l-POPNA conjugated with a cell-penetrating peptide (antisense reagent). The antisense effect of the conjugated POPNA (inhibition of LacZ activity) was comparable to that shown by a Nielsen-type peptide nucleic acid. Furthermore, the conjugated POPNA could switch the LacZ activity over a wide range of ambient temperatures.
Department of Medical and Bioengineering, Graduate School of Natural Science and Technology, Okayama University, 3-1-1 Tsushimanaka, Kita-ku, Okayama 700-0082, Japan. kitamatu@cc.okayama-u.ac.jp
Mizuki
M
Kitamatsu
Shunsuke
S
Kurami
Takashi
T
Ohtsuki
Masahiko
M
Sisido
D000595:Amino Acid Sequence; D004926:Escherichia coli / Q000187:drug effects*; D016376:Oligonucleotides, Antisense / Q000737:chemistry* / Q000494:pharmacology; D020135:Peptide Nucleic Acids / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D011759:Pyrrolidines / Q000737:chemistry*
D016376:Oligonucleotides, Antisense; D020135:Peptide Nucleic Acids; D011759:Pyrrolidines