Klysimplexins I-T, eunicellin-based diterpenoids from the cultured soft coral Klyxum simplex.
New eunicellin-base diterpenoids, klysimplexins I-T (1-12), were isolated from a cultured soft coral Klyxum simplex. Their structures were elucidated by spectroscopic methods, particularly in 1D and 2D NMR experiments. The absolute stereochemistry of 4 was determined by Mosher's method. Compounds 9 and 12 have been shown to exhibit cytotoxicity toward a limited panel of cancer cell lines. Compounds 2-6, 10 and 11 were found to display significant in vitro anti-inflammatory activity in LPS-stimulated RAW264.7 macrophage cells by inhibiting the expression of the iNOS protein. Compounds 10 and 11 also could effectively reduce the level of COX-2 protein.
Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 804, Taiwan.
Bo-Wei
BW
Chen
Chih-Hua
CH
Chao
Jui-Hsin
JH
Su
Chung-Wei
CW
Tsai
Wei-Hsien
WH
Wang
Zhi-Hong
ZH
Wen
Chiung-Yao
CY
Huang
Ping-Jyun
PJ
Sung
Yang-Chang
YC
Wu
Jyh-Horng
JH
Sheu
D000818:Animals; D037421:Anthozoa / Q000737:chemistry*; D002460:Cell Line; D051546:Cyclooxygenase 2 / Q000378:metabolism; D004224:Diterpenes / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000201:enzymology; D051379:Mice; D008958:Models, Molecular; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism
D004224:Diterpenes; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse; C077623:Ptgs2 protein, mouse; D051546:Cyclooxygenase 2