Detection of organophosphate pesticide using polyaniline and carbon nanotubes composite based on acetylcholinesterase inhibition.
Acetylcholinesterase (AChE) activity may be useful biomarker for detecting of organophosphate pesticides (OP). Thus a sensitive biosensor for quantitative determination of OP based on AChE biomonitoring was developed. Multi-walled carbon nanotube (MWCNT)/polyaniline (PANI) composite film was prepared by electrochemical polymerization. The immobilized AChE catalyzed the hydrolysis of acetylthiocholine chloride to produce thiocholine, which engendered an irreversible oxidation peak. The enzyme activity was monitored by measuring the oxidation current of thiocholine and further detection of OP. The developed sensor provided a new promising tool for pesticide analysis and assay of enzyme activity.
Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, Central China Normal University, Wuhan 430079, PR China.
Daiqin
D
Chen
Chuanhuizi
C
Chen
Dan
D
Du
D000110:Acetylcholinesterase; D000814:Aniline Compounds / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods; D002800:Cholinesterase Inhibitors / Q000032:analysis; D004563:Electrochemistry; D004800:Enzymes, Immobilized; D008294:Malathion / Q000032:analysis; D053761:Nanocomposites / Q000737:chemistry; D036103:Nanotechnology; D037742:Nanotubes, Carbon; D009943:Organophosphorus Compounds / Q000032:analysis*; D010575:Pesticides / Q000032:analysis*
D000814:Aniline Compounds; D002800:Cholinesterase Inhibitors; D004800:Enzymes, Immobilized; D037742:Nanotubes, Carbon; D009943:Organophosphorus Compounds; D010575:Pesticides; C416807:polyaniline; D000110:Acetylcholinesterase; D008294:Malathion