Arginine-glycine-aspartic acid-conjugated dendrimer-modified quantum dots for targeting and imaging melanoma.
Angiogenesis is essential for the development of malignant tumors and provides important targets for tumor diagnosis and therapy. Quantum dots have been broadly investigated for their potential application in cancer molecular imaging. In present work, CdSe quantum dots were synthesized, polyamidoamine dendrimers were used to modify surface of quantum dots and improve their solubility in water solution. Then, dendrimer-modified CdSe quantum dots were conjugated with arginine-glycine-aspartic acid (RGD) peptides. These prepared nanoprobes were injected into nude mice loaded with melanoma (A375) tumor xenografts via tail vessels, IVIS imaging system was used to image the targeting and bio-distribution of as-prepared nanoprobes. The dendrimer-modified quantum dots exhibit water-soluble, high quantum yield, and good biocompatibility. RGD-conjugated quantum dots can specifically target human umbilical vein endothelial cells (HUVEC) and A375 melanoma cells, as well as nude mice loaded with A735 melanoma cells. High-performance RGD-conjugated dendrimers modified quantum dot-based nanoprobes have great potential in application such as tumor diagnosis and therapy.
Institute of Dermatology and Department of Dermatology at No. 1 Hospital, Anhui Medical University, The Key Laboratory of Gene Resource Utilization for Severe Diseases, Ministry of Education, Hefei 230032, China.
Zhiming
Z
Li
Peng
P
Huang
Jing
J
Lin
Rong
R
He
Bing
B
Liu
Xiaomin
X
Zhang
Sen
S
Yang
Peng
P
Xi
Xuejun
X
Zhang
Qiushi
Q
Ren
Daxiang
D
Cui
D000818:Animals; D045744:Cell Line, Tumor; D002470:Cell Survival; D050091:Dendrimers / Q000737:chemistry* / Q000493:pharmacokinetics; D016503:Drug Delivery Systems; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D008545:Melanoma / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D008819:Mice, Nude; D008856:Microscopy, Fluorescence; D057054:Molecular Imaging / Q000379:methods*; D058726:Nanoconjugates / Q000737:chemistry; D009842:Oligopeptides / Q000737:chemistry* / Q000493:pharmacokinetics; D045663:Quantum Dots; D013050:Spectrometry, Fluorescence; D014018:Tissue Distribution; D051598:Whole Body Imaging; D023041:Xenograft Model Antitumor Assays
D050091:Dendrimers; D058726:Nanoconjugates; D009842:Oligopeptides; C104700:PAMAM Starburst; C047981:arginyl-glycyl-aspartic acid