Upregulated INHBA expression is associated with poor survival in gastric cancer.
Expression microarrays are widely used for investigating the candidate molecular targets in human cancer. While genome-wide expression signatures screened by gene set enrichment analysis (GSEA) were not performed in Chinese gastric cancer (GC). To gain new molecular targets for GC, GSEA analysis was performed. In the present study, GSEA were used to pick out differentially expressed gene sets of our database. Total RNA of paired tissue samples (n = 48) and a tissue microarray containing 132 paired tissues were used to further validate expression levels of INHBA and its correction with clinicopathological factors. Upregulated INHBA expression in gastric cancer was screened and further confirmed by qPCR and immunostaining analysis. Increased INHBA expression was significantly correlated with the diameter of cancer and depth of tumor invasion. Patients with higher expression levels of INHBA had a shorter disease-free survival rate. It was effective to gain new molecular targets for GC by GSEA analysis. INHBA may be a poor survival indicator of GC.
Department of General Surgery, Shanghai First People's Hospital, College of Medicine, Shanghai Jiao Tong University, 85 Wujin Road, 200080 Shanghai, China.
Quan
Q
Wang
Yu-Gang
YG
Wen
Da-Peng
DP
Li
Jun
J
Xia
Chong-Zhi
CZ
Zhou
Dong-Wang
DW
Yan
Hua-Mei
HM
Tang
Zhi-Hai
ZH
Peng
D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis*; D018572:Disease-Free Survival; D005260:Female; D020869:Gene Expression Profiling; D055106:Genome-Wide Association Study; D006801:Humans; D007150:Immunohistochemistry; D028322:Inhibin-beta Subunits / Q000378:metabolism*; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D020411:Oligonucleotide Array Sequence Analysis; D011379:Prognosis; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000401:mortality*; D046888:Tissue Array Analysis
D014408:Biomarkers, Tumor; C103008:inhibin beta A subunit; D028322:Inhibin-beta Subunits