Hippocampal CARP over-expression solidifies consolidation of contextual fear memories.
The Doublecortin-Like Kinase (DCLK) gene is involved in neuronal migration during development. Through alternative splicing the DCLK gene also produces a transcript called Ca(2+)/calmodulin dependent protein kinase (CaMK)-related peptide (CARP) that is expressed exclusively during adulthood in response to neuronal activity. The function of CARP, however, is poorly understood. To study CARP function, we have generated transgenic mice with over-expression of the CARP transcript in, amongst other brain areas, the hippocampus. We aimed to characterize possible behavioral adaptations of these mice by using a Pavlovian fear conditioning approach. This type of fear conditioning, in which both the hippocampus and amygdala are critically involved, allows studying the formation and extinction of fear related memories. We here report on the behavioral adaptations of two distinct transgenic lines: one with high levels of CARP in the hippocampus and amygdala, whilst the other has high levels of CARP in the hippocampal formation, but not in the amygdala. We tested both mouse lines separately by comparing them to their wild-type littermate controls. We provide evidence suggesting consolidation of contextual fear memories is strengthened in mice of both transgenic lines.
Division of Medical Pharmacology, Leiden/Amsterdam Centre for Drug Research, Einsteinweg 55, 2333 CC Leiden, The Netherlands. G.J.Schenk@lacdr.leidenuniv.nl
Geert J
GJ
Schenk
Erno
E
Vreugdenhil
Chantal J Y
CJ
Hubens
Barbera
B
Veldhuisen
E Ron
ER
de Kloet
Melly S
MS
Oitzl
D000679:Amygdala / Q000502:physiology; D000704:Analysis of Variance; D000818:Animals; D002352:Carrier Proteins; D003214:Conditioning, Classical / Q000502:physiology*; D003345:Corticosterone / Q000097:blood; D005239:Fear / Q000502:physiology*; D006624:Hippocampus / Q000502:physiology*; D008568:Memory / Q000502:physiology*; D051379:Mice; D008822:Mice, Transgenic; D058748:Pain Perception / Q000502:physiology; D010750:Phosphoproteins / Q000378:metabolism*; D011863:Radioimmunoassay
D002352:Carrier Proteins; D010750:Phosphoproteins; C461219:RNF34 protein, human; D003345:Corticosterone