Frequent detection of Human Herpes Virus-8 in bone marrow of Jordanian patients of multiple myeloma.
The association between Human Herpes Virus-8 (HHV-8), also called Kaposi's sarcoma associated herpesvirus (KSHV), and the pathogenesis of multiple myeloma remains controversial. Many past studies conducting on different populations have come to contradicting conclusions. In this study, we attempted to investigate the presence of HHV-8 in Jordanian multiple myeloma patients. We carried out nucleic acid amplification reactions targeting specific viral DNA sequences on 35 fresh bone marrow aspirate samples from 17 patients with multiple myeloma, 9 patients with various hematological malignancies and 9 normal subjects. HHV-8 specific sequences were detected in 7 out of 17 multiple myeloma patients (41%) using primers specific for the open reading frame region 26 (ORF26). All patients with other hematological malignancies as well as the normal subjects did not harbour the virus. These findings support the previous reports of frequent detection of HHV-8 in bone marrow of multiple myeloma patients.
Molecular Biology Research Lab, Department of Biochemistry, Faculty of Medicine, University of Jordan, Amman 11942, Jordan. sismail@ju.edu.jo
Said I
SI
Ismail
Ismail S
IS
Mahmoud
Mohammad A L
MA
Salman
Maher A
MA
Sughayer
Azmi M
AM
Mahafzah
D000328:Adult; D000368:Aged; D001853:Bone Marrow / Q000473:pathology* / Q000821:virology; D016022:Case-Control Studies; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D005500:Follow-Up Studies; D019337:Hematologic Neoplasms / Q000175:diagnosis / Q000235:genetics / Q000821:virology; D019288:Herpesvirus 8, Human / Q000235:genetics* / Q000472:pathogenicity; D006801:Humans; D015994:Incidence; D007597:Jordan; D008297:Male; D008403:Mass Screening; D008875:Middle Aged; D009101:Multiple Myeloma / Q000175:diagnosis* / Q000235:genetics / Q000821:virology*; D009367:Neoplasm Staging; D016133:Polymerase Chain Reaction; D011379:Prognosis; D012307:Risk Factors; D012514:Sarcoma, Kaposi / Q000175:diagnosis* / Q000235:genetics / Q000821:virology; D015996:Survival Rate
D004279:DNA, Viral