Characterization of two urease-producing and calcifying Bacillus spp. isolated from cement.
Two bacterial strains designated as CT2 and CT5 were isolated from highly alkaline cement samples using the enrichment culture technique. On the basis of various physiological tests and 16S rRNA sequence analysis, the bacteria were identified as Bacillus species. The urease production was 575.87 U/ml and 670.71 U/ml for CT2 and CT5 respectively. Calcite constituted 27.6% and 31% of the total weight of sand samples plugged by CT2 and CT5, respectively. Scanning electron micrography (SEM) analysis revealed the direct involvement of these isolates in calcite precipitation. This is the first report of the isolation and identification of Bacillus species from cement. Based on the ability of these bacteria to tolerate extreme environment of cement, they have potential to be used in remediating the cracks and fissures in various building or concrete structures.
Department of Biotechnology, Thapar University, Patiala 147 004, India. varenyam@gmail.com
Varenyam
V
Achal
Abhijit
A
Mukherjee
M Sudhakara
MS
Reddy
D001407:Bacillus / Q000145:classification / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002119:Calcium Carbonate / Q000032:analysis / Q000378:metabolism*; D003252:Construction Materials / Q000032:analysis / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012823:Silicate Cement / Q000032:analysis*; D014510:Urease / Q000235:genetics / Q000378:metabolism*
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012823:Silicate Cement; D014510:Urease; D002119:Calcium Carbonate