Preparation of low-molecular-mass peptides by enzyme autolysis oF Paphia undulata.
Paphia undulata was used to prepare low-molecular-mass peptides by enzyme autolysis. The optimum condition for autolysis was studied to reach a maximum yield of peptides. Results showed that the yield of low-molecular-mass peptides was 4.33% and 78.57% of the total protein can be recovered under the optimized condition: pH = 6.5, temperature = 45 °C, reaction time = 4 h, ratio of starting material and water = 1:3 and the enzyme dosage was 1374.0 U/g.
Department of Chemistry and Chemical Engineering, Foshan University, Foshan, P.R. China. fschenxin@tom.com
Chen
C
Xin
Sun
S
Huili
Zhou
Z
Debo
D000818:Animals; D049872:Bivalvia / Q000737:chemistry / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D013696:Temperature; D013997:Time Factors
D010455:Peptides