DNA barcode-based molecular identification system for fish species.
In this study, we applied DNA barcoding to identify species using short DNA sequence analysis. We examined the utility of DNA barcoding by identifying 53 Korean freshwater fish species, 233 other freshwater fish species, and 1339 saltwater fish species. We successfully developed a web-based molecular identification system for fish (MISF) using a profile hidden Markov model. MISF facilitates efficient and reliable species identification, overcoming the limitations of conventional taxonomic approaches. MISF is freely accessible at http://bioinfosys.snu.ac.kr:8080/MISF/misf.jsp .
Interdisciplinary Program in Bioinformatics, Seoul National University, Seoul 151-742, Korea.
Sungmin
S
Kim
Hae-Seok
HS
Eo
Hyeyoung
H
Koo
Jun-Kil
JK
Choi
Won
W
Kim
D000818:Animals; D019295:Computational Biology; D004247:DNA / Q000235:genetics; D058893:DNA Barcoding, Taxonomic / Q000379:methods*; D015342:DNA Probes / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics*; D030541:Databases, Genetic; D001330:Electronic Data Processing / Q000379:methods; D005399:Fishes / Q000145:classification* / Q000235:genetics*; D010802:Phylogeny; D017422:Sequence Analysis, DNA / Q000379:methods; D013045:Species Specificity
D015342:DNA Probes; D004272:DNA, Mitochondrial; D004247:DNA