[PCR-RAPD typing of carbapenem-resistant Pseudomonas aeruginosa strains].
P. aeruginosa rods are opportunistic pathogens responsible generally for nosocomial infections. Resistance to carbapenems, observed among them, is a serious threat due to ability to be transmitted between bacterial species. The aim of our study was to evaluate the usefulness of PCR-RAPD technique in typing of 16 carbapenem-resistant P. aeruginosa strains isolated in 2007 from different patients of University HospitalNo. 1 of dr A. Jurasz Collegium Medicum of L. Rydygier in Bydgoszcz Nicolaus Copernicus University in Toruń. Study shows increasing frequency of isolation that type of strains when compared to 2006. Percentage of carbapenem-resistant isolates raised from 12,4% in 2006 to 22.9% in 2007. The majority of examined strains were obtained from patients of the Intensive Care Units (25.0%) and were isolated from bronchoalveolar lavage (25.0%), urine (25.0%) and wound swabs (18.8%) samples. Examined P. aeruginosa strains demonstrated resistance to doripenem (81.3%) and piperacillin (75.0%) and susceptibility to colistin (100.0%), amikacin (81.3%), netilmicin and norfloxacin (75.0% each). Using PCR-RAPD amplification with 208 and 272 primers, 14 and 16 DNA patterns were obtained, respectively. Usefulness of PCR-RAPD in carbapenem-resistant P. aeruginosa strains typing was proved in case of strains presenting similar and/or different antimicrobials susceptibility patterns.
Katedra i Zakład Mikrobiologii, Collegium Medicum im. L. Rydygiera w Bydgoszczy, Uniwersytet Mikołaja Kopernika w Toruniu.
Tomasz
T
Bogiel
Eugenia
E
Gospodarek
D015373:Bacterial Typing Techniques / Q000379:methods*; D001992:Bronchoalveolar Lavage Fluid / Q000382:microbiology; D015780:Carbapenems / Q000494:pharmacology*; D006801:Humans; D011550:Pseudomonas aeruginosa / Q000145:classification* / Q000187:drug effects* / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*; D013045:Species Specificity; D014556:Urine / Q000382:microbiology; D014947:Wounds and Injuries / Q000382:microbiology; D018440:beta-Lactam Resistance
D015780:Carbapenems