Individual specific extraction of DNA from male mixtures--First evaluation studies.
In forensic work, the interpretation of DNA profiles becomes complicated when samples contain more than one contributor because the simultaneous amplification of individual identification markers results in mixed profiles. To overcome this problem, we present haplotype-specific extraction (HSE) as a more straightforward method to analyze a DNA mixture. HSE has been developed to clarify ambiguous HLA alleles by separating diploid samples into their haploid components to facilitate HLA typing. We have started to establish new protocols and strategies to adapt HSE for the separation of male DNA mixtures in forensic analysis. First results have shown an improved enrichment of male DNA from a single contributor. We have also evaluated a new, optimized buffer composition by testing different concentrations of its components. Improved separation of a male DNA mixture is detected using AmpFℓSTR(®) Yfiler short tandem repeat analysis.
Department of Forensic Genetics, Institute of Legal Medicine and Forensic Sciences, Charité- Campus Virchow-Klinikum, Augustenburger Platz 1, Forum 4, 13353 Berlin, Germany. jessica.rothe@charite.de
Jessica
J
Rothe
Lutz
L
Roewer
Marion
M
Nagy
D041322:Chromosomes, Human, Y; D004247:DNA / Q000302:isolation & purification*; D016172:DNA Fingerprinting / Q000379:methods*; D017931:DNA Primers; D015342:DNA Probes; D006239:Haplotypes; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA; D020080:Tandem Repeat Sequences
D017931:DNA Primers; D015342:DNA Probes; D004247:DNA