Calreticulin expression in neoplastic versus normal dog mammary glands: a cDNA subtraction-based study.
This is the first report describing the expression of canine calreticulin (cCRT) in canine mammary gland tumour (MGT). Using cDNA subtraction method, it is found that mRNAs of CRT, cathepsin A, ovostatin, and lactotransferrin were differentially expressed in mammary adenocarcinoma as compared to hyperplasia, both of which were obtained from the dog. Furthermore, the mRNA expression levels of CRT and cathepsin A were significantly higher in canine MGT samples than in nontumour samples. In contrast, immunohistochemical studies have indicated that the expression of cCRT protein found to be detected in most of mammary gland tissues and was not correlated to the types of canine MGTs. Furthermore, cCRT was molecularly cloned, and the amino acid sequence of cCRT was found to be very similar to those of other species. Further studies are required to elucidate additional roles of cCRT in canine MGT.
Department of Veterinary Medicine, Yamaguchi University, Yamaguchi, Japan.
Takumi
T
Okawa
Yuzo
Y
Kurio
Masahiro
M
Morimoto
Toshiharu
T
Hayashi
Takayuki
T
Nakagawa
Nobuo
N
Sasaki
Masaru
M
Okuda
Takuya
T
Mizuno
D000230:Adenocarcinoma / Q000378:metabolism / Q000662:veterinary*; D000818:Animals; D001483:Base Sequence; D014408:Biomarkers, Tumor / Q000378:metabolism*; D015153:Blotting, Western / Q000662:veterinary; D037282:Calreticulin / Q000235:genetics / Q000378:metabolism*; D043402:Cathepsin A / Q000378:metabolism; D045744:Cell Line, Tumor; D004283:Dog Diseases / Q000378:metabolism*; D004285:Dogs; D005260:Female; D015723:Gene Library; D007781:Lactoferrin / Q000378:metabolism; D015674:Mammary Neoplasms, Animal / Q000378:metabolism*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D000511:alpha-Macroglobulins / Q000378:metabolism
D014408:Biomarkers, Tumor; D037282:Calreticulin; D012333:RNA, Messenger; D000511:alpha-Macroglobulins; C036828:ovomacroglobulin; D043402:Cathepsin A; D007781:Lactoferrin