Arthroderma benhamiae (the teleomorph of Trichophyton mentagrophytes) mating type-specific genes.
This study first report to identify the mating type (-)-specific gene of alpha-box and the mating type (+)-specific gene of the high-mobility-group (HMG) DNA-binding domain in zoophilic dermatophytes of Arthroderma benhamiae in an effort to understand the epidemiological characteristics of Trichophyton mentagrophytes. The sequence of the alpha-box gene (1,387 bp) was found to contain two exons, from 184 to 475 bp and from 525 to 1,387 bp, coding a protein of 384 amino acids, beginning with a putative initiating methionine (ATG). The sequence of the HMG gene (1,910 bp) contained two exons, from 234 to 415 bp and from 479 to 1,457 bp, coding a protein of 386 amino acids, beginning with a putative initiating methionine (ATG). PCR analysis detected the alpha-box gene in A. benhamiae (-) mating type strains but not in (+) mating type strains. On the other hand, the HMG gene was detected in A. benhamiae (+) mating type strains but not in (-) mating type strains. These findings suggest that the HMG and alpha-box genes could be specific to the (+) and (-) mating types, respectively.
Department of Pathobiology, Nihon University School of Veterinary Medicine, 1866, Kameino, Fujisawa, Kanagawa, 252-0880, Japan. kano@brs.nihon-u.ac.jp
Rui
R
Kano
Tsuyoshi
T
Yamada
Koichi
K
Makimura
Masako
M
Kawasaki
Takashi
T
Mochizuki
Hiroshi
H
Kamata
Atsuhiko
A
Hasegawa
D000595:Amino Acid Sequence; D000818:Animals; D003883:Arthrodermataceae / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D006224:Cricetinae; D003881:Dermatomycoses / Q000382:microbiology; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics; D049770:Genes, Mating Type, Fungal; D008969:Molecular Sequence Data; D011817:Rabbits; D016415:Sequence Alignment; D014005:Tinea / Q000382:microbiology; D014249:Trichophyton / Q000737:chemistry / Q000235:genetics* / Q000502:physiology
D005656:Fungal Proteins