Tissue proteome profiling of preeclamptic placenta using recombinant antibody microarrays.
OBJECTIVE
preeclampsia (PE) is a severe, multi-system pregnancy disorder of yet unknown cause, missing means of treatment, and our fundamental understanding of the disease is still impaired. The purpose of this discovery study was to define candidate placenta tissue protein biomarker signatures to further decipher the molecular features of PE.


METHODS
we used recombinant antibody microarrays for multiplexed protein expression profiling of preeclamptic placenta tissue (n=25) versus normal placenta (n=11) targeting mainly immunoregulatory water-soluble proteins and membrane proteins. Furthermore, the three known subgroups of PE were profiled, including women with early onset preeclampsia and late onset preeclampsia, as well as women with PE and bilateral notching and intrauterine growth restrictions.


RESULTS
the data showed that the first generation of candidate PE-associated placenta tissue protein signatures were delineated, indicating that PE (receiver operating characteristics (ROC) AUC value of 0.83) and the subgroups thereof (ROC AUC values ≤ 0.91) could be distinguished. Notably, the data implied that all subgroups, but preeclampsia with bilateral notching and IUGR, could be further classified into novel subsets (ROC AUC values of 1.0) displaying different inflammatory signatures.


CONCLUSIONS
we have taken one step further toward de-convoluting the complex features of PE at the molecular level using affinity proteomics.
Department of Immunotechnology, Lund University, Lund, Sweden.
Linda
L
Dexlin-Mellby
Anna
A
Sandström
Magnus
M
Centlow
Sara
S
Nygren
Stefan R
SR
Hansson
Carl A K
CA
Borrebaeck
Christer
C
Wingren
D000328:Adult; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods*; D005865:Gestational Age; D006801:Humans; D008875:Middle Aged; D010920:Placenta / Q000378:metabolism* / Q000473:pathology; D011225:Pre-Eclampsia / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D011247:Pregnancy; D040081:Protein Array Analysis / Q000379:methods*; D020543:Proteome / Q000032:analysis*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D055815:Young Adult
D020543:Proteome; D011994:Recombinant Proteins