Preparation and characterization of culture of CD146+ cells from human adipose tissue.
A method for isolation of homogenous culture of cells expressing CD146 marker from adipose tissue lipoaspirate was developed. The resultant clonogenic cultures retained high proliferative activity, immunophenotype, and morphology after numerous passages. The presence of insulin in the medium served as the selective factor for maintenance of the population phenotype. These cultures effectively differentiate into CD31(+)endothelial cells and can be used in regenerative medicine.
ReMeTex Company, Moscow, Russia. rzhaninova@rm7
A A
AA
Rzhaninova
A V
AV
Kulikov
I A
IA
Spirova
E E
EE
Kirienko
A V
AV
Volkov
D V
DV
Goldshtein
D000273:Adipose Tissue / Q000166:cytology*; D000328:Adult; D051929:CD146 Antigen / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping; D008875:Middle Aged; D039902:Multipotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D017154:Stromal Cells / Q000166:cytology* / Q000378:metabolism
D051929:CD146 Antigen; C497902:MCAM protein, human