Advanced follicle development in xenografted prepubertal ovarian tissue: the common marmoset as a nonhuman primate model for ovarian tissue transplantation.
OBJECTIVE
To establish a nonhuman primate model addressing follicular development in cryopreserved prepubertal ovarian tissue after xenografting.


METHODS
Experimental study.


METHODS
Academic research center.


METHODS
Ovarian tissue from female prepubertal common marmoset (Callytrix jacchus jacchus) grafted into immunodeficient nude mice (Crl:NU-FoxnI(nu)).


METHODS
Removal and subsequent cryopreservation of ovarian tissues with dimethyl sulfoxide, followed by grafting to subcutaneous sites of ovariectomized and intact nude mice.


METHODS
Histologic evaluation for the mean number of total and morphologically normal follicles in each class.


RESULTS
The mean number of unadvanced follicles in frozen-thawed grafted ovarian tissues was reduced compared with pregraft controls, but the prevalence of normal follicular morphology was either slightly increased (primordial follicles) or unchanged (primary follicles). Previous ovariectomy in graft recipients increased total follicle numbers without effect on normal follicular morphology and shifted the ratio of primordial to primary follicles toward an increase in primary follicles, indicating activation of follicular maturation.


CONCLUSIONS
The marmoset is a suitable primate model for studies on the subsequent use of cryopreserved ovarian tissue, demonstrating graft sustainment and the development of follicles from prepubertal ovarian tissue in immunodeficient hosts up to secondary and preantral stages.
Department of Obstetrics and Gynecology, Campus Grosshadern, Ludwig-Maximilians-Universität Munich, Munich, Germany.
Viktoria
V
von Schönfeldt
Ramesh
R
Chandolia
Ludwig
L
Kiesel
Eberhard
E
Nieschlag
Stefan
S
Schlatt
Barbara
B
Sonntag
D000367:Age Factors; D000818:Animals; D002144:Callithrix / Q000254:growth & development*; D005260:Female; D051379:Mice; D008819:Mice, Nude; D023421:Models, Animal; D006080:Ovarian Follicle / Q000166:cytology / Q000254:growth & development* / Q000637:transplantation*; D016378:Tissue Transplantation / Q000379:methods; D014183:Transplantation, Heterologous