Inhibition of viral adsorption and penetration by an aqueous extract from Rhododendron ferrugineum L. as antiviral principle against herpes simplex virus type-1.
The polyphenol-enriched aqueous extract RF from the aerial parts of Rhododendron ferrugineum exhibited strong antiviral activity against herpes simplex virus type-1 while adenovirus 3 was not affected. RF exhibited an IC(50) of 7.4 μg/mL and a selectivity index of 64 when added to the virus inoculum prior to infection. RF abolished virus entry into the host cell by blocking attachment to the cell surface. When added after attachment at a concentration of >25 μg/mL, RF inhibited also penetration of HSV-1 into the host cell. RF directly interacts with viral envelope proteins as demonstrated for the viral glycoprotein gD.
Institute of Pharmaceutical Biology and Phytochemistry, University of Münster, Muenster, Germany.
Kirsten
K
Gescher
Joachim
J
Kühn
Wali
W
Hafezi
Andreas
A
Louis
Andrea
A
Derksen
Alexandra
A
Deters
Eva
E
Lorentzen
Andreas
A
Hensel
D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology / Q000627:therapeutic use*; D002522:Chlorocebus aethiops; D006023:Glycoproteins / Q000378:metabolism; D006561:Herpes Simplex / Q000188:drug therapy* / Q000378:metabolism / Q000821:virology; D018259:Herpesvirus 1, Human / Q000187:drug effects*; D020128:Inhibitory Concentration 50; D008517:Phytotherapy; D035261:Plant Components, Aerial; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D029793:Rhododendron; D014709:Vero Cells; D014759:Viral Envelope Proteins / Q000378:metabolism; D053585:Virus Attachment / Q000187:drug effects*
D000998:Antiviral Agents; D006023:Glycoproteins; D010936:Plant Extracts; D014759:Viral Envelope Proteins