Enhanced Th17 phenotype in uninfected neonates born from viremic HIV-1-infected pregnant women.
Our objective was to evaluate the in vitro functional profile of T cells from uninfected neonates born from HIV-1-infected pregnant women who controlled (G1) or not (G2) the virus replication. We demonstrated that the lymphoproliferation of T cell to polyclonal activators was higher in the G2 as compared with G1. Nevertheless, no detectable proliferative response was observed in response to HIV-1 antigens in both neonate groups. Cytokine dosage in the supernatants of these polyclonally activated T cell cultures demonstrated that, while IL-10 was the dominant cytokine produced in G1, Th17-related cytokines were significantly higher in G2 neonates. The higher Th17 phenotype tendency in G2 was related to high production of IL-23 by lipopolysaccharide-activated monocyte-derived dendritic cells from these neonates. Our results demonstrated immunological disorders in uninfected neonates born from viremic HIV-1-infected mothers that can help to explain why some of these children have elevated risk of clinical morbidity and mortality due to pathological hypersensitivity.
Department of Microbiology and Parasitology, Federal University of the State of Rio de Janeiro, Frei Caneca 94, 20.261-040, Rio de Janeiro, Rio de Janeiro, Brazil.
Joana
J
Hygino
Morgana M
MM
Vieira
Landi V
LV
Guillermo
Renato G
RG
Silva-Filho
Carmen
C
Saramago
Agostinho A
AA
Lima-Silva
Regis M
RM
Andrade
Arnaldao F B
AF
Andrade
Rodrigo M
RM
Brindeiro
Amilcar
A
Tanuri
Vander
V
Guimarães
Cleonice Alves
CA
de Melo Bento
D000328:Adult; D049109:Cell Proliferation; D002478:Cells, Cultured; D016207:Cytokines / Q000097:blood / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology; D005260:Female; D005312:Fetal Blood / Q000166:cytology; D015658:HIV Infections / Q000276:immunology*; D006679:HIV Seropositivity / Q000276:immunology; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D007231:Infant, Newborn; D007963:Leukocytes, Mononuclear / Q000276:immunology; D018655:Lymphocyte Count; D011247:Pregnancy; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology; D058504:Th17 Cells / Q000276:immunology*; D055815:Young Adult
D016207:Cytokines