Bioconversion of artemisinin to its nonperoxidic derivative deoxyartemisinin through suspension cultures of Withania somnifera Dunal.
Biotransformation of artemisinin was investigated with two different cell lines of suspension cultures of Withania somnifera. Both cell lines exhibited potential to transform artemisinin into its nonperoxidic analogue, deoxyartemisinin, by eliminating the peroxo bridge of artemisinin. The enzyme involved in the reaction is assumed to be artemisinin peroxidase, and its activity in extracts of W. somnifera leaves was detected. Thus, the non-native cell-free extract of W. somnifera and suspension culture-mediated bioconversion can be a promising tool for further manipulation of pharmaceutical compounds.
Central Institute of Medicinal and Aromatic Plants (CIMAP-CSIR), PO CIMAP, Lucknow-226015, UP, India.
Farzana
F
Sabir
Anil
A
Kumar
Pragya
P
Tiwari
Neelam
N
Pathak
Rajender S
RS
Sangwan
Rajendra S
RS
Bhakuni
Neelam S
NS
Sangwan
D037621:Artemisinins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D001711:Biotransformation; D002474:Cell-Free System; D002478:Cells, Cultured; D002855:Chromatography, Thin Layer; D008516:Medicine, Chinese Traditional; D008958:Models, Molecular; D032341:Withania / Q000166:cytology / Q000378:metabolism*
D037621:Artemisinins; C055261:deoxyartemisinin; C031327:artemisinin