Subcellular distribution of polyhydroxylated metallofullerene Gd@C82(OH)22 in different tissues of tumor-bearing mice.
Endohedral metallofullerenes have unique chemical and physical properties that are essential for biomedical applications. They can be used as a radiotracer in magnetic resonance and X-ray imaging (MRI and XRI). Polyhydroxylated metallofullerene Gd@C82(OH)x has been studied as a new high efficient contrast agents for MRI and has also shown high efficiency for antitumor growth. Previous studies have shown that Gd@C82(OH)22 mainly accumulated in bone, pancreas, liver and kidney after intraperitoneal administration. However, whether the nanoparticles can enter into cells and the site of their potential action remains unclear. In this work, the distribution patterns of Gd@C82(OH)22 in subcellular compartments were studied using the techniques of differential centrifugation and ICP-MS analysis. The results indicate that the Gd@C82(OH)22 can enter into cells and the subcellular distribution patterns are significantly different from that of GdCl3. GdCl3 has much higher bioavailability for mice than Gd@C82(OH)22. Therefore, the present data suggest that Gd@C82(OH)22 in living systems may be not decomposed to release free Gd3+ ion and may be metabolized as a pristine carbon cage.
CAS Key Lab for Biological Effects of Nanomaterials and Nanosafety, Institute of High Energy Physics, Chinese Academy of Sciences and National Center for Nanoscience and Technology of China.
Guoqiang
G
Zhou
Yufeng
Y
Li
Ying
Y
Liu
Cuicui
C
Ge
Wei
W
Li
Baoyun
B
Sun
Bai
B
Li
Yuxi
Y
Gao
Chunying
C
Chen
D000818:Animals; D001682:Biological Availability; D002498:Centrifugation; D005260:Female; D037741:Fullerenes / Q000737:chemistry / Q000493:pharmacokinetics*; D005682:Gadolinium / Q000737:chemistry / Q000493:pharmacokinetics*; D042541:Intracellular Space / Q000737:chemistry / Q000378:metabolism*; D008114:Liver Neoplasms, Experimental / Q000378:metabolism*; D013058:Mass Spectrometry; D051379:Mice; D053761:Nanocomposites / Q000737:chemistry*; D009942:Organometallic Compounds / Q000493:pharmacokinetics*; D014018:Tissue Distribution; D014183:Transplantation, Heterologous
D037741:Fullerenes; D009942:Organometallic Compounds; D005682:Gadolinium