Optimization of culture media of pathogenic Mycoplasma hyopneumoniae by a response surface methodology.
Composition of culture medium for mass production of Mycoplasma hyopneumoniae was optimized using a response surface methodology (RSM). Initially, the influence of glucose, thallium acetate, fresh yeast extract, horse serum, and porcine serum on the production of mycoplasmal protein was assessed using a 'one factor at a time' technique. Next, factors with a significant effect, including fresh yeast extract, and horse and porcine sera, were selected for further optimization using a central composite design (CCD) of RSM. The experimental results were fitted into a second order polynomial model equation. Estimated optimal condition of the factors for maximum production of mycoplasmal protein (i.e., triple-fold increase from 0.8 mg/L produced by basal mycoplasma media to 2.5 mg/L) was 10.9% fresh yeast extract, 15% horse serum, and 31.5% porcine serum (v/v). For the optimized conditions, a 2.96 mg/L experimental result was observed, similar to the estimated optimal conditions result of the CCD.
College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Korea.
Mi-Hyun
MH
Hwang
Dereje
D
Damte
Min-Hee
MH
Cho
Young-Hoan
YH
Kim
Seung-Chun
SC
Park
D001709:Biotechnology / Q000379:methods*; D003470:Culture Media / Q000737:chemistry*; D045705:Mycoplasma hyopneumoniae / Q000254:growth & development*
D003470:Culture Media