Pentagalloylglucose downregulates cofilin1 and inhibits HSV-1 infection.
To investigate the anti-herpesvirus mechanism of pentagalloylglucose (PGG), we compared the proteomic changes between herpes simplex virus type 1 (HSV-1) infected MRC-5 cells with or without PGG-treatment, and between non-infected MRC-5 cells with or without PGG-treatment by 2-DE and MS-based analysis. Differentially expressed cellular proteins were mainly involved with actin cytoskeleton regulation. Significantly, PGG can down-regulate cofilin1, a key regulator of actin cytoskeleton dynamics. PGG can inhibit HSV-1-induced rearrangements of actin cytoskeleton which is important for infectivity. Furthermore, cofilin1 knockdown by siRNA also inhibited the HSV-1-induced actin-skeleton rearrangements. Both PGG-treatment and cofilin1 knockdown can reduce HSV-1 DNA, mRNA, protein synthesis and virus yields. Altogether, the results suggested that down-regulating cofilin1 plays a role in PGG inhibiting HSV-1 infection. PGG may be a promising anti-herpesvirus agent for drug development.
Biomedicine Research and Development Center of Jinan University, Guangzhou Huangpu Road West 601, Guangzhou, Guangdong 510632, China.
Ying
Y
Pei
Yang-Fei
YF
Xiang
Jia-Nan
JN
Chen
Chun-Hua
CH
Lu
Jing
J
Hao
Qian
Q
Du
Chi-choi
CC
Lai
Chang
C
Qu
Shen
S
Li
Huai-Qiang
HQ
Ju
Zhe
Z
Ren
Qiu-Ying
QY
Liu
Sheng
S
Xiong
Chui-Wen
CW
Qian
Fan-Li
FL
Zeng
Pei-Zhuo
PZ
Zhang
Chong-Ren
CR
Yang
Ying-Jun
YJ
Zhang
Jun
J
Xu
Kaio
K
Kitazato
Yi-Fei
YF
Wang
D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D051338:Cofilin 1 / Q000037:antagonists & inhibitors* / Q000096:biosynthesis / Q000235:genetics; D015536:Down-Regulation; D055785:Gene Knockdown Techniques; D018259:Herpesvirus 1, Human / Q000502:physiology*; D006801:Humans; D047348:Hydrolyzable Tannins / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000378:metabolism; D053586:Virus Internalization / Q000187:drug effects*
D051338:Cofilin 1; D047348:Hydrolyzable Tannins; D034741:RNA, Small Interfering; C435084:pentagalloylglucose