Enhancing collection efficiency in large field of view multiphoton microscopy.
Many multiphoton imaging applications would benefit from a larger field of view; however, large field of views (>mm) require low magnification objectives which have low light collection efficiencies. We demonstrate a light collection system mounted on a low magnification objective that increases fluorescence collection by as much as 20-fold in scattering tissues. This peripheral detector results in an effective numerical aperture of collection >0.8 with a 3-4 mm field of view.
School of Applied and Engineering Physics, Cornell University, Ithaca, NY, USA.
J D
JD
McMullen
A C
AC
Kwan
R M
RM
Williams
W R
WR
Zipfel
D000008:Abdominal Neoplasms / Q000473:pathology; D000818:Animals; D001921:Brain / Q000166:cytology; D002277:Carcinoma / Q000473:pathology; D051379:Mice; D008856:Microscopy, Fluorescence / Q000379:methods*; D010336:Pathology / Q000379:methods*; D013116:Spinal Cord / Q000166:cytology