Functionalized quantum dots to quantify NADPH and their use for NADP+-dependent biocatalyzed transformations.
Quantum dots are semiconducting nanoparticles that can be prepared with interesting optical properties. The fluorescent properties of quantum dots are one of the key advantages for their use as optical labels for biorecognition events and biocatalytic processes. We have prepared semiconductor quantum dots conjugated with Nile Blue (NB), and demonstrate that NB-functionalized quantum dots can act as versatile probes to analyze different biocatalyzed transformations, and can be used for the quantitative detection of NADPH as well as NADH. This approach provides a new path for the optical detection of NAD(P)H and for the quantitative analysis of NAD(P)(+)-dependent biotransformations.
Department of Chemistry and Research Institute for Basic Sciences, Kyung Hee University, Seoul, 130-701, Korea.
Min Young
MY
Kim
Yea Seul
YS
Kim
Jisu
J
Kim
Sang Soo
SS
Hah
Tae Jung
TJ
Kim
Young Dong
YD
Kim
D009249:NADP / Q000737:chemistry*; D045663:Quantum Dots
D009249:NADP