RNA aptazyme-tethered large gold nanoparticles for on-the-spot sensing of the aptazyme ligand.
A single-step sensing system was developed to visually detect ligands of a cleavase-like RNA aptazyme at room temperature using aptazyme-tethered gold nanoparticles, the electrosteric stability of which was adjusted by increasing their diameter. In this system, the ligand induces self-cleavage of the aptazyme on gold nanoparticles to decrease the electrosteric stability of the gold nanoparticles, which causes them to visibly aggregate. In comparison to a previous multi-step system using aptazymes and gold nanoparticles separately, the present system requires only single handling and no special equipment, making it more suitable for on-the-spot sensing.
Ehime University, 3 Bunkyo-cho, Matsuyama, Ehime 790-8577, Japan. a-ogawa@ccr.ehime-u.ac.jp
Atsushi
A
Ogawa
D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques; D006046:Gold / Q000737:chemistry*; D008024:Ligands; D053768:Metal Nanoparticles / Q000737:chemistry*; D016337:RNA, Catalytic / Q000737:chemistry* / Q000378:metabolism; D055672:Static Electricity; D013696:Temperature
D052157:Aptamers, Nucleotide; D008024:Ligands; D016337:RNA, Catalytic; D006046:Gold