Porous scaffolds based on cross-linking of poly(L-glutamic acid).
Porous scaffolds based on water-soluble PLGA and CS were prepared. The pores were verified to be alveolate, uniform and continuous. The effects of freezing temperature, freeze-drying time, solid content and molecular weight of reactants on the pore structure of the scaffolds were studied. The scaffold morphology could be adjusted by changing the freezing temperature and solid content of reacting polymer. Their degradation rate can be adjusted by changing the proportion of PLGA and CS. The porosity of scaffolds was higher than 90% and the high swelling ratio showed that these scaffolds had excellent hydrophilic performance. The in vitro culture of chondrocytes indicates that the obtained PLGA/CS porous scaffolds are very promising biomaterials for tissue engineering applications.
Department of Polymer Materials, Shanghai University, 20 Chengzhong Road, Jiading, Shanghai, China.
Bin
B
Cao
Jingbo
J
Yin
Shifeng
S
Yan
Lei
L
Cui
Xuesi
X
Chen
Yongtao
Y
Xie
D000818:Animals; D048271:Chitosan / Q000737:chemistry / Q000494:pharmacology; D019902:Chondrocytes / Q000166:cytology / Q000187:drug effects; D003432:Cross-Linking Reagents / Q000737:chemistry*; D005612:Freeze Drying; D005615:Freezing; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D008970:Molecular Weight; D011099:Polyglutamic Acid / Q000737:chemistry* / Q000494:pharmacology; D016062:Porosity; D013499:Surface Properties / Q000187:drug effects; D013997:Time Factors; D054457:Tissue Scaffolds / Q000737:chemistry*; D014961:X-Ray Diffraction
D003432:Cross-Linking Reagents; D011099:Polyglutamic Acid; D048271:Chitosan