Over-expression of talin 1 and integrin-linked kinase in PBMCs of patients with ankylosing spondylitis: a proteomic study.
OBJECTIVE
Ankylosing spondylitis (AS) is difficult to diagnose in its early stage due to the lack of simple and specific diagnostic indicators. This study was performed to screen candidate AS-associated proteins from peripheral blood mononuclear cells (PBMCs) of AS patients by combining a two-dimensional electrophoresis (2-DE) technique with mass spectrometry (MS) analysis.


METHODS
Twelve subjects consisting of 6 AS patients and 6 healthy volunteers (HVs) were enrolled in the 2-DE experiments. The protein expression patterns of PBMCs from different groups were analysed by PDQuest software, and the protein spots over/under-expressed by more than 2-fold between the two groups were identified by MS analysis. Western blot analyses were used to verify the differentially expressed proteins in 32 AS patients and 32 HVs.


RESULTS
Six proteins including pyruvate kinase (PK), profilin 1 (PFN1), talin 1 (TLN1), Chain A of cyclophilin A (CyPA), unknown protein (gi|16306948) and integrin-linked kinase (ILK) were identified from 10 over-expressed protein spots found by 2-DE in the AS group. Western blot experiments confirmed a higher expression of both TLN1 and ILK in AS group compared to the HV group (p<0.05).


CONCLUSIONS
TLN1 and ILK expressed higher in PBMCs of AS patients compared to healthy controls, which were involved in the integrin signalling pathway. The two proteins are likely novel disease-associated proteins and potential disease markers of AS.
Department of Rheumatology, Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China.
T
T
Li
B
B
Zheng
Z
Z
Huang
H
H
Lu
Q
Q
Lin
Z
Z
Liao
Z
Z
Lin
L
L
Zhao
X
X
Wang
J
J
Gu
D000328:Adult; D015415:Biomarkers / Q000097:blood; D015153:Blotting, Western; D016022:Case-Control Studies; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D015203:Reproducibility of Results; D015398:Signal Transduction / Q000502:physiology; D013167:Spondylitis, Ankylosing / Q000097:blood* / Q000503:physiopathology; D016608:Talin / Q000378:metabolism*; D000099200:Scaffold Protein ILK
D015415:Biomarkers; D017346:Protein Serine-Threonine Kinases; D016608:Talin; D000099200:Scaffold Protein ILK; C518832:TLN1 protein, human