A sub-nanomole level electrochemical method for determination of prochloraz and its metabolites based on medical stone doped disposable electrode.
A ultrasensitive, simple and convenient electrochemical method was firstly developed for the determination of prochloraz and its metabolites as 2,4,6-trichlorophenol (2,4,6-TCP) using nano-aperture medical stone. Compared with the undoped disposable electrode (UDE), nano-aperture medical stone doped disposable electrode (MSDDE) not only significantly enhances the oxidation peak current of 2,4,6-TCP but also lowers the oxidation overpotential, suggesting that the nano-aperture MSDDE can remarkably improve the sensitivity of 2,4,6-TCP. The experimental conditions such as pH values of buffer solution, the content of nano-aperture medical stone, accumulation potential and time were optimized for the determination of 2,4,6-TCP. At optimal conditions, the oxidation peak current is proportional to the concentration of 2,4,6-TCP over the range from 6.0 × 10(-9) to 8.0 × 10(-5)mol L(-1). Finally, this novel method was successfully employed to detect prochloraz and its metabolites in orange rind with the detection limit of 8.4 × 10(-10)mol L(-1) (0.3 ng g(-1)) and the method was validated by gas chromatography.
College of Chemistry and Engineering, Yangzhou University, 88 South University Avenue, Yangzhou 225002, PR China.
Hongbo
H
Li
Jing
J
Li
Chuantao
C
Hou
Shi
S
Du
Yanyan
Y
Ren
Zhanjun
Z
Yang
Qin
Q
Xu
Xiaoya
X
Hu
D002138:Calibration; D002623:Chemistry Techniques, Analytical; D002733:Chlorophenols / Q000737:chemistry; D002849:Chromatography, Gas / Q000379:methods; D032084:Citrus sinensis / Q000378:metabolism; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D006863:Hydrogen-Ion Concentration; D007093:Imidazoles / Q000032:analysis* / Q000737:chemistry; D046529:Microscopy, Electron, Transmission / Q000379:methods
D002733:Chlorophenols; D007093:Imidazoles; C045362:prochloraz; C024564:2,4,6-trichlorophenol