Amplified electrochemical DNA sensor using peroxidase-like DNAzyme.
A novel electrochemical DNA sensor was developed here by using peroxidase-like G-quadruplex-based DNAzyme as a biocatalytic label. A hairpin structure including the G-quadruplex-based DNAzyme in a caged configuration and the target DNA probe were immobilized on Au-electrode surface. Upon hybridization with the target, the hairpin structure was opened, and the G-quadruplex-based DNAzyme was generated on the electrode surface, triggering the electrochemical oxidization of hydroquinone by H(2)O(2), which provide a quantitative measure for the detection of the target DNA. The DNA target was analyzed with a detection limit of 0.6 nM. This method is simple and easy to design without direct conjugation of redox-active element.
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China.
Qiuping
Q
Guo
Yanyan
Y
Bao
Xiaohai
X
Yang
Kemin
K
Wang
Qing
Q
Wang
Yuyu
Y
Tan
D015374:Biosensing Techniques / Q000379:methods*; D002384:Catalysis; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D021881:DNA, Catalytic / Q000737:chemistry*; D004563:Electrochemistry / Q000379:methods*; D054856:G-Quadruplexes; D006861:Hydrogen Peroxide / Q000737:chemistry; D006873:Hydroquinones / Q000737:chemistry; D008957:Models, Genetic; D009693:Nucleic Acid Hybridization; D009696:Nucleic Acids / Q000737:chemistry; D009841:Oligonucleotides / Q000235:genetics; D013997:Time Factors
D021881:DNA, Catalytic; D006873:Hydroquinones; D009696:Nucleic Acids; D009841:Oligonucleotides; D004247:DNA; D006861:Hydrogen Peroxide; C031927:hydroquinone