Comparison of AOAC 2005.06 LC official method with other methodologies for the quantitation of paralytic shellfish poisoning toxins in UK shellfish species.
A refined version of the pre-column oxidation liquid chromatography with fluorescence detection (ox-LC-FLD) official method AOAC 2005.06 was developed in the UK and validated for the determination of paralytic shellfish poisoning toxins in UK shellfish. Analysis was undertaken here for the comparison of PSP toxicities determined using the LC method for a range of UK bivalve shellfish species against the official European reference method, the PSP mouse bioassay (MBA, AOAC 959.08). Comparative results indicated a good correlation in results for some species (mussels, cockles and clams) but a poor correlation for two species of oysters (Pacific oysters and native oysters), where the LC results in terms of total saxitoxin equivalents were found to be on average more than double the values determined by MBA. With the potential for either LC over-estimation or MBA under-estimation, additional oyster and mussel samples were analysed using MBA and ox-LC-FLD together with further analytical and functional methodologies: a post-column oxidation LC method (LC-ox-FLD), an electrophysiological assay and hydrophilic interaction liquid chromatography with tandem mass spectrometric detection. Results highlighted a good correlation among non-bioassay results, indicating a likely cause of difference was the under-estimation in the MBA, rather than an over-estimation in the LC results.
Centre for Environment, Fisheries and Aquaculture Science (Cefas), Barrack Road, The Nothe, Weymouth, Dorset DT4 8UB, UK. andrew.turner@cefas.co.uk
Andrew D
AD
Turner
Robert G
RG
Hatfield
Monika
M
Rapkova
Wendy
W
Higman
Myriam
M
Algoet
Benjamin A
BA
Suarez-Isla
Marco
M
Cordova
Catherine
C
Caceres
Jeffrey
J
van de Riet
Ryan
R
Gibbs
Krista
K
Thomas
Michael
M
Quilliam
David N
DN
Lees
D000818:Animals; D002853:Chromatography, Liquid; D005504:Food Analysis / Q000379:methods*; D008387:Marine Toxins / Q000032:analysis*; D015203:Reproducibility of Results; D057096:Shellfish Poisoning; D013045:Species Specificity; D013050:Spectrometry, Fluorescence; D006113:United Kingdom
D008387:Marine Toxins