Inhibition by polycyclic aromatic hydrocarbons of ATPase activities in Sebastiscus marmoratus larvae: relationship with the development of early life stages.
Sebastiscus marmoratus larvae were exposed to waterborne polycyclic aromatic hydrocarbons (PAHs) containing 3-5 rings, benzo[a]pyrene (BaP), pyrene (Py) or phenanthrene (Phe), respectively at 0.01, 0.1 and 1 μg L⁻¹. Cumulative mortality, frequency of dorsal curvature and rate of pericardial and yolk sac edema in larvae treated for 8 days were significantly increased in a dose-dependent manner. All three PAHs resulted in reduction of the lower jaw, and the extent of reduction increased with increasing concentrations of the PAHs. Na+/K+-ATPase and Ca²+-ATPase activity in larvae exposed to the three PAHs were all significantly inhibited in a dose-dependent manner. Analysis using the Pearson correlation coefficient indicated a significant correlation between the rate of the dorsal curvature and edema and the inhibition of Na+/K+-ATPase and Ca²+-ATPase activity, suggesting that the developmental defects caused by PAHs were related to their inhibition of Na+/K+-ATPase and Ca²+-ATPase activity.
Key Laboratory of Ministry of Education for Subtropical Wetland Ecosystem Research, School of Life Sciences, Xiamen University, Xiamen 361005, PR China.
Ruixia
R
Li
Zhenghong
Z
Zuo
Donglei
D
Chen
Chengyong
C
He
Rong
R
Chen
Yixin
Y
Chen
Chonggang
C
Wang
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D004791:Enzyme Inhibitors / Q000633:toxicity*; D005399:Fishes / Q000002:abnormalities / Q000378:metabolism*; D007814:Larva / Q000187:drug effects / Q000201:enzymology; D009026:Mortality; D011084:Polycyclic Aromatic Hydrocarbons / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000633:toxicity
D004791:Enzyme Inhibitors; D011084:Polycyclic Aromatic Hydrocarbons; D014874:Water Pollutants, Chemical; D000251:Adenosine Triphosphatases