Thermoresponsible cell adhesion/detachment on transparent nanocomposite films consisting of poly(2-methoxyethyl acrylate) and clay.
Soft, transparent and mechanically tough nanocomposite (M-NC) films composed of poly(2-methoxyethyl acrylate) (PMEA) and inorganic clay (hectorite) were studied as substrates for a living cell harvest system. It was found that five cell types could all be cultivated to confluence on the surface of M-NC n films with clay content (n = 10-23 wt%), although the cells hardly cultivated on the surface of chemically-cross-linked PMEA and linear PMEA films. Further, it was found that the cells cultured on the surfaces of M-NC films can be detached, without any enzymatic digestion, by decreasing the medium temperature and/or simultaneously using gentle pipetting. The detached cell was obtained as a single cell or a contiguous cell sheet, both of which were viable and recultured. From the compositions and surface properties, it was estimated that the cell culture and subsequent cell detachment were attributed to the unique PMEA/clay network. The new soft nanocomposite is potentially a very promising substrate for tissue engineering.
a Material Chemistry Laboratory, Kawamura Institute of Chemical Research, 631 Sakado, Sakura, Chiba 285-0078, Japan. hara@kicr.or.jp.
Kazutoshi
K
Haraguchi
Sakie
S
Masatoshi
Noriko
N
Kotobuki
Kazutaka
K
Murata
D000179:Acrylates / Q000737:chemistry; D000538:Aluminum Silicates / Q000737:chemistry; D000818:Animals; D041702:BALB 3T3 Cells; D002417:Cattle; D002448:Cell Adhesion / Q000502:physiology; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods; D002470:Cell Survival; D000077215:Clay; D056945:Hep G2 Cells; D006801:Humans; D008422:Materials Testing; D051379:Mice; D053761:Nanocomposites / Q000737:chemistry / Q000648:ultrastructure; D011108:Polymers / Q000737:chemistry; D013499:Surface Properties; D013696:Temperature; D013718:Tensile Strength; D023822:Tissue Engineering; D014867:Water / Q000737:chemistry
D000179:Acrylates; D000538:Aluminum Silicates; D011108:Polymers; C413485:poly(2-methoxyethylacrylate); D014867:Water; D000077215:Clay