Training in the fasted state facilitates re-activation of eEF2 activity during recovery from endurance exercise.
Nutrition is an important co-factor in exercise-induced training adaptations in muscle. We compared the effect of 6 weeks endurance training (3 days/week, 1-2 h at 75% VO(2peak)) in either the fasted state (F; n = 10) or in the high carbohydrate state (CHO, n = 10), on Ca(2+)-dependent intramyocellular signalling in young male volunteers. Subjects in CHO received a carbohydrate-rich breakfast before each training session, as well as ingested carbohydrates during exercise. Before (pretest) and after (posttest) the training period, subjects performed a 2 h constant-load exercise bout (~70% of pretest VO(2peak)) while ingesting carbohydrates (1 g/kg h(-1)). A muscle biopsy was taken from m. vastus lateralis immediately before and after the test, and after 4 h of recovery. Compared with pretest, in the posttest basal eukaryotic elongation factor 2 (eEF2) phosphorylation was elevated in CHO (P < 0.05), but not in F. In the pretest, exercise increased the degree of eEF2 phosphorylation about twofold (P < 0.05), and values returned to baseline within the 4 h recovery period in each group. However, in the posttest dephosphorylation of eEF2 was negated after recovery in CHO, but not in F. Independent of the dietary condition training enhanced the basal phosphorylation status of Phospholamban at Thr(17), 5'-AMP-activated protein kinase α (AMPKα), and Acetyl CoA carboxylase β (ACCβ), and abolished the exercise-induced increase of AMPKα and ACCβ (P < 0.05). In conclusion, training in the fasted state, compared with identical training with ample carbohydrate intake, facilitates post-exercise dephosphorylation of eEF2. This may contribute to rapid re-activation of muscle protein translation following endurance exercise.
Department of Biomedical Kinesiology, Research Centre for Exercise and Health, FaBeR-K.U. Leuven, Tervuursevest 101, 3001 Leuven, Heverlee, Belgium.
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D000328:Adult; D004040:Dietary Carbohydrates / Q000494:pharmacology; D054736:Elongation Factor 2 Kinase / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects / Q000502:physiology; D015444:Exercise / Q000502:physiology*; D005080:Exercise Test; D005215:Fasting / Q000502:physiology*; D006801:Humans; D008297:Male; D020652:Peptide Elongation Factor 2 / Q000378:metabolism*; D010766:Phosphorylation / Q000187:drug effects; D010806:Physical Education and Training; D010807:Physical Endurance / Q000187:drug effects / Q000502:physiology*; D020127:Recovery of Function / Q000187:drug effects / Q000502:physiology*; D055815:Young Adult
D004040:Dietary Carbohydrates; D020652:Peptide Elongation Factor 2; C517689:EEF2K protein, human; D054736:Elongation Factor 2 Kinase