Evaluation of the effects of Candidatus Liberibacter asiaticus on inoculated citrus plants using laser-induced breakdown spectroscopy (LIBS) and chemometrics tools.
This study investigated the organic and inorganic constituents of healthy leaves and Candidatus Liberibacter asiaticus (CLas)-inoculated leaves of citrus plants. The bacteria CLas are one of the causal agents of citrus greening (or Huanglongbing) and its effect on citrus leaves was investigated using laser-induced breakdown spectroscopy (LIBS) combined with chemometrics. The information obtained from the LIBS spectra profiles with chemometrics analysis was promising for the construction of predictive models to identify healthy and infected plants. The major, macro- and microconstituents were relevant for differentiation of the sample conditions. The models were then applied to different inoculation times (from 1 to 8 months). The models were effective in the classification of 82-97% of the diseased samples with a 95% significance level. The novelty of this method was in the fingerprinting of healthy and diseased plants based on their organic and inorganic contents.
Embrapa Instrumentação Agropecuária, P.O. Box 741, Zip Code 13561-206, São Carlos, SP, Brazil. fmverbi@uol.com.br
Fabíola Manhas Verbi
FM
Pereira
Débora Marcondes Bastos Pereira
DM
Milori
André Leonardo
AL
Venâncio
Mariana de Sá Tavares
Mde S
Russo
Polyana Kelly
PK
Martins
Juliana
J
Freitas-Astúa
D002623:Chemistry Techniques, Analytical; D002957:Citrus / Q000382:microbiology*; D004247:DNA / Q000378:metabolism; D007834:Lasers; D008027:Light; D010935:Plant Diseases; D018515:Plant Leaves / Q000378:metabolism*; D018521:Plant Physiological Phenomena; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012230:Rhizobiaceae / Q000378:metabolism*; D013053:Spectrophotometry / Q000379:methods*; D013997:Time Factors
D004247:DNA