Enzymatic deposition of Au nanoparticles on the designed electrode surface and its application in glucose detection.
This paper reported the enzymatic deposition of Au nanoparticles (AuNPs) on the designed 3-mercapto-propionic acid/glucose oxidase/chitosan (MPA/GOD/Chit) modified glassy carbon electrode and its application in glucose detection. Chit served as GOD immobilization matrix and interacted with MPA through electrostatic attraction. AuNPs, without nano-seeds presented on the electrode surface, was produced through the glucose oxidase catalyzed oxidation of glucose. The mechanism of production of AuNPs was confirmed to be that enzymatic reaction products H(2)O(2) in the solution reduce gold complex to AuNPs. The characterizations of the electrode modified after each assembly step was investigated by cyclic voltammetry and electrochemical impedance spectroscopy. Scanning electron microscopy showed the average particle size of the AuNPs is 40nm with a narrow particle size distribution. The content of AuNPs on the electrode surfaces was measured by differential pulse stripping voltammetry. The electrochemical signals on voltammogram showed a linear increase with the glucose concentration in the range of 0.010-0.12mM with a detection limit of 4μM. This provided a method to the determination of glucose.
Key Laboratory of Synthetic and Natural Functional Molecule Chemistry, Ministry of Education, Department of Chemistry, Northwest University, Xi'an 710069, PR China.
Hongfang
H
Zhang
Ruixiao
R
Liu
Qinglin
Q
Sheng
Jianbin
J
Zheng
D015374:Biosensing Techniques / Q000379:methods*; D002384:Catalysis; D004563:Electrochemistry / Q000379:methods; D004566:Electrodes; D005947:Glucose / Q000032:analysis* / Q000737:chemistry; D005949:Glucose Oxidase / Q000032:analysis; D006046:Gold / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning / Q000379:methods; D036103:Nanotechnology / Q000379:methods*; D010100:Oxygen / Q000737:chemistry; D010316:Particle Size; D055672:Static Electricity
D006046:Gold; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D005947:Glucose; D010100:Oxygen