Acidovorax radicis sp. nov., a wheat-root-colonizing bacterium.
Strain N35(T) was isolated from surface-sterilized wheat roots and is a Gram-negative, aerobic, motile straight rod. Strain N35(T) tested oxidase-positive and catalase-negative and grew optimally at pH 7.0, 30 °C and in the absence of NaCl. 16S rRNA gene sequence analysis showed over 97 % sequence similarity to strains of the environmental species Acidovorax delafieldii, A. facilis, A. defluvii, A. temperans, A. caeni and A. soli, as well as Acidovorax valerianellae, A. anthurii and Simplicispira metamorpha. DNA-DNA hybridization between strain N35(T) and phylogenetically closely related type strains was 25.3-55.7 %, which clearly separates the strain from these closely related species. Additionally, phenotypic properties, such as substrate metabolism profiles as determined by a Biolog GN2 assay and cell-wall fatty acid profiles, particularly contents of the fatty acids C(16 : 0), C(16 : 1)ω7c/t, C(17 : 0), C(17 : 0) cyclo, C(18 : 0) cyclo and C(19 : 0) cyclo, facilitated the differentiation of the newly isolated strain N35(T) from its closest relatives. The isolate underwent phenotypic variation at high frequency in laboratory media. The DNA G+C content was 64.9 mol%. We propose that strain N35(T) is classified as a representative of a novel species within the genus Acidovorax, and suggest the name Acidovorax radicis sp. nov. The type strain is strain N35(T) ( = DSM 23535(T) = LMG 25767(T)).
Helmholtz Zentrum Muenchen, German Research Center for Environmental Health (GmbH), Department Microbe-Plant Interactions, Ingolstaedter Landstrasse 1, Neuherberg, Germany.
Dan
D
Li
Helmholtz Zentrum Muenchen, German Research Center for Environmental Health (GmbH), Department Microbe-Plant Interactions, Ingolstaedter Landstrasse 1, Neuherberg, Germany.
Michael
M
Rothballer
Helmholtz Zentrum Muenchen, German Research Center for Environmental Health (GmbH), Department Microbe-Plant Interactions, Ingolstaedter Landstrasse 1, Neuherberg, Germany.
Michael
M
Schmid
Helmholtz Zentrum Muenchen, German Research Center for Environmental Health (GmbH), Institute of Soil Ecology, Department Terrestrial Ecogenetics, Ingolstaedter Landstrasse 1, Neuherberg, Germany.
Jürgen
J
Esperschütz
Helmholtz Zentrum Muenchen, German Research Center for Environmental Health (GmbH), Department Microbe-Plant Interactions, Ingolstaedter Landstrasse 1, Neuherberg, Germany.
Anton
A
Hartmann
D001482:Base Composition; D042621:Comamonadaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012965:Sodium Chloride / Q000378:metabolism; D012988:Soil Microbiology; D014908:Triticum / Q000382:microbiology*
D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride