Frequency of HLA-G exon 8 polymorphisms and kidney allograft outcome in Iranian population.
The 14-bp polymorphism in exon 8 of the HLA-G gene is associated with HLA-G mRNA stability and the patterns of alternative isoform splicing and may influence the functionality of the HLA-G molecule. HLA-G expression was related to allograft acceptance and fewer episodes of acute rejection during heart, kidney and liver-kidney transplantation. In order to determine a possible correlation between the 14-bp insertion/deletion polymorphism and kidney allograft outcome in our population, genomic DNA was isolated from 144 patients who had received isolated kidney allografts. The recipients was divided into two groups, grafts presenting features of rejection group and a non-rejection group, and compared them with a control group of 100 healthy subjects. There was no significant difference in allelic frequencies of 14-bp insertion/deletion polymorphism between normal controls and kidney transplant patients. No significant difference was found between the RG and the NRG regarding the 14-bp genotypes and alleles. Therefore, additional studies with more sample size from other populations with analysis of other HLA-G polymorphisms are necessary to define this polymorphism as a valuable clinical marker.
Organ Transplant Research Center, Nemazi Hospital, Shiraz University of Medical Sciences, Zand Street, 7193711351 Shiraz, Iran.
Mahdokht H
MH
Aghdaie
Negar
N
Azarpira
Kurosh
K
Kazemi
Bita
B
Geramizadeh
Masumeh
M
Darai
Seid Ali
SA
Malekhoseini
D000328:Adult; D005091:Exons; D005260:Female; D005838:Genotype; D006084:Graft Rejection / Q000235:genetics* / Q000276:immunology*; D006680:HLA Antigens / Q000235:genetics*; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D006801:Humans; D007492:Iran; D016030:Kidney Transplantation / Q000276:immunology*; D008297:Male; D011110:Polymorphism, Genetic; D020871:RNA Stability / Q000235:genetics; D014184:Transplantation, Homologous / Q000276:immunology*; D016896:Treatment Outcome; D055815:Young Adult
D006680:HLA Antigens; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I