Resveratrol inhibits isoprostane production in young and aged rat brain.
Resveratrol (3,5,4-trihydroxystilbene), a viniferin polyphenolic compound, has been shown to have neuroprotective effects and we tested its possible antioxidant activity in young and aged rat brain, evaluating, in vitro, synaptosomal 8-iso-prostaglandin F2alpha (8-iso-PGF2alpha) production as a marker of oxidative stress. We found that in young rat brain synaptosomes resveratrol perfusion had no effect on basal 8-iso-PGF2alpha production, but quenched to basal levels the increased 8-iso-PGF2alpha production induced by hydrogen peroxide. On the other hand, in aged rats, resveratrol was able to decrease 8-iso-PGF2alpha production both basally and after hydrogen peroxide-induced oxidative stimulus. In conclusion, our findings show that the antioxidant effects of resveratrol in rat brain could play a neuroprotective role in aging, when the increased burden of oxidative stress is faced by defective antioxidant mechanisms.
Department of Drug Sciences, G. d'Annunzio University, Chieti, Italy.
A
A
Chiavaroli
L
L
Brunetti
G
G
Orlando
L
L
Recinella
C
C
Ferrante
S
S
Leone
P
P
Di Michele
C
C
Di Nisio
M
M
Vacca
D000375:Aging / Q000378:metabolism*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001921:Brain / Q000187:drug effects* / Q000378:metabolism*; D015237:Dinoprost / Q000031:analogs & derivatives / Q000096:biosynthesis; D006861:Hydrogen Peroxide / Q000633:toxicity; D028421:Isoprostanes / Q000096:biosynthesis*; D008297:Male; D018696:Neuroprotective Agents / Q000494:pharmacology; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*; D013574:Synaptosomes / Q000187:drug effects / Q000378:metabolism
D000975:Antioxidants; D028421:Isoprostanes; D018696:Neuroprotective Agents; D013267:Stilbenes; C075750:8-epi-prostaglandin F2alpha; D015237:Dinoprost; D006861:Hydrogen Peroxide; D000077185:Resveratrol