Gustducin is expressed in the taste buds of the chicken.
We investigated the expression of gustducin in chicken taste buds using molecular biological, biochemical and immunohistochemical techniques. Expression of a gustducin-like sequence was detected by RT-PCR in the tissues containing taste buds, and corresponded to the predicted gustducin gene in the chicken. Expression of this sequence was not detected in the brain, heart, liver, pancreas, intestine, kidney and testis of the chicken. The expressed sequence had a high specificity for oral tissues that contained taste buds. These results suggest that the detected sequence was the chicken gustducin gene. Next, we generated a polyclonal antiserum against the chicken gustducin protein to observe its localization in the oral tissues. The results revealed that the chicken gustducin was specifically expressed in the taste buds. It is suggested that the chicken has a gustatory system mediated by gustducin, and chicken gustducin is a reliable marker for taste buds or taste cells. This is the first molecular biological, biochemical and immunohistochemical demonstration of the presence of gustducin in the chicken.
Department of Animal and Marine Bioresource Sciences, Kyushu University, Higashi, Fukuoka, Japan.
Ken-ichi
K
Kudo
Kei-ichi
K
Wakamatsu
Shotaro
S
Nishimura
Shoji
S
Tabata
D000818:Animals; D015153:Blotting, Western; D002645:Chickens / Q000378:metabolism*; D015870:Gene Expression; D007150:Immunohistochemistry; D009928:Organ Specificity; D012313:RNA / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013650:Taste Buds / Q000378:metabolism*; D015293:Transducin / Q000032:analysis* / Q000235:genetics
C075192:gustducin; D012313:RNA; D015293:Transducin