Metallo beta-lactamase producing pseudomonas species--a major cause of concern among hospital associated urinary tract infection.
Multidrug resistant pseudomonas strains are isolated in high frequency from urinary samples in hospitalised patients. With the occurrence of extended spectrum beta-lactamases (ESBLs) and metallo beta-lactamase (MBL)-producing Pseudomonas aeruginosa being increasingly reported worldwide, this study evaluated the resistance pattern of pseudomonad strains to carbapenam and other antipseudomonal antibiotics isolated from patients with hospital associated urinary tract infections along with clinical usefulness of various MBL screening methods (combined disc diffusion test, E-test and modified Hodge test). Presence of ESBL and AmpC beta-lactamases in the isolates was also determined. Of the 87 isolates, 81 (93.1%) were Pseudomonas aeruginosa, 4 (4.6%) were Pseudomonas putida, 2 (2.3%) were Burkholderia cepacia. Thirty-one isolates (35.6%) were resistant to imipenem and 61% of these 31 isolates, were MBL producers by combined disc diffusion test, while 48% were detected by E-test method. Overall, in 30% and 54% strains, production of AmpC and ESBL respectively was observed.
Department of Microbiology, University College of Medical Sciences and Guru Teg Bahadur Hospital, Delhi 110095.
Rumpa
R
Saha
Sarika
S
Jain
Iqbal R
IR
Kaur
D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D001426:Bacterial Proteins / Q000096:biosynthesis; D003428:Cross Infection / Q000188:drug therapy / Q000201:enzymology* / Q000382:microbiology*; D024901:Drug Resistance, Multiple, Bacterial; D006801:Humans; D007194:India; D008826:Microbial Sensitivity Tests; D011446:Prospective Studies; D011552:Pseudomonas Infections / Q000201:enzymology* / Q000382:microbiology*; D014552:Urinary Tract Infections / Q000188:drug therapy / Q000382:microbiology*; D001618:beta-Lactamases / Q000096:biosynthesis*
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; C048795:AmpC beta-lactamases; D001618:beta-Lactamases