Rapid PCR detection of Staphylococcus aureus clonal complex 398 by targeting the restriction-modification system carrying sau1-hsdS1.
A PCR targeting sau1-hsdS1 was developed for rapid detection of Staphylococcus aureus clonal complex 398 (CC398). High sensitivity (100%) and specificity (100%) were shown by evaluating the test on a large strain collection (n = 1,307). We recommend this test for accurate, rapid, and inexpensive diagnosis of methicillin-resistant S. aureus (MRSA) CC398 in hospitals and on farms.
Department of Microbiological Surveillance and Research, Statens Serum Institut, 5 Artillerivej, DK-2300 Copenhagen S, Denmark. mtg@ssi.dk
Marc
M
Stegger
Jodi A
JA
Lindsay
Arshnee
A
Moodley
Robert
R
Skov
Els M
EM
Broens
Luca
L
Guardabassi
D000818:Animals; D015373:Bacterial Typing Techniques / Q000379:methods*; D015280:DNA Restriction-Modification Enzymes / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013203:Staphylococcal Infections / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*
D015280:DNA Restriction-Modification Enzymes