Combining laser capture microdissection and proteomics: methodologies and clinical applications.
Laser capture microdissection (LCM) has become an important tool for biomedical research. Various molecular biology techniques have been combined with LCM to reveal molecular profiles at an unprecedented tissue resolution. Proteomics is among those techniques that have proved fruitful in combination with LCM. This review provides an overview of the various proteomic techniques that have been developed for analyzing cells obtained using LCM. Methods for analyzing microdissected cells for various proteomic techniques are described and compared. In addition, various applications based on LCM and proteomics are discussed.
Department of Neurological Surgery, Vanderbilt University School of Medicine, Nashville, TN, USA. baogang.j.xu@vanderbilt.edu
Baogang J
BJ
Xu
D000818:Animals; D035843:Biomedical Research / Q000379:methods*; D002623:Chemistry Techniques, Analytical; D006801:Humans; D007834:Lasers; D042282:Microdissection / Q000379:methods*; D040081:Protein Array Analysis; D040901:Proteomics / Q000379:methods*