A novel drug and gene co-delivery system based on Poly(epsilon-caprolactone)-Poly(ethylene glycol)-Poly(epsilon-caprolactone) grafted polyethyleneimine micelle.
In this paper, we prepared a novel cationic self-assembled micelle from poly(epsilon-caprolactone)-poly(ethyl glycol)-poly(epsilon-caprolactone) grafted polyethyleneimine (PCEC-g-PEI). The PCEC-g-PEI micelles, formed by self-assembly method, had mean particle size of ca. 82 nm and zeta potential of +22.5 mV at 37 degrees C, and could efficiently transfer pGFP into HEK293 cells in vitro. Meanwhile, as a model hydrophobic chemotherapeutic drug, honokiol was loaded into PCEC-g-PEI micelles by direct dissolution method assisted by ultrasonication. The honokiol loaded cationic PCEC-g-PEI micelles could effectively adsorb DNA onto its surface, while it could release honokiol in an extended period in vitro. This study demonstrated a novel DNA and hydrophobic chemotherapeutic drug co-delivery system.
State Key Laboratory of Biotherapy and Cancer Center West China Hospital, West China Medical School, Sichuan University, Chengdu, 610041, China.
Ke
K
Men
Ma Ling
ML
Gou
Qing Fa
QF
Guo
Xiu Hong
XH
Wang
Shuai
S
Shi
Bing
B
Kan
Mei Juan
MJ
Huang
Feng
F
Luo
Li Juan
LJ
Chen
Xia
X
Zhao
Zhi Yong
ZY
Qian
Shu Fang
SF
Liang
Yu Quan
YQ
Wei
D001713:Biphenyl Compounds / Q000493:pharmacokinetics; D002470:Cell Survival; D004247:DNA / Q000008:administration & dosage / Q000737:chemistry / Q000235:genetics; D016503:Drug Delivery Systems / Q000379:methods*; D018014:Gene Transfer Techniques; D057809:HEK293 Cells; D006801:Humans; D017705:Lignans / Q000493:pharmacokinetics; D008823:Micelles; D058726:Nanoconjugates / Q000737:chemistry*; D010316:Particle Size; D011091:Polyesters / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D011094:Polyethyleneimine / Q000737:chemistry*; D013696:Temperature; D000498:Allyl Compounds; D010636:Phenols
D001713:Biphenyl Compounds; D004247:DNA; D017705:Lignans; D008823:Micelles; D058726:Nanoconjugates; D011091:Polyesters; D011092:Polyethylene Glycols; D011094:Polyethyleneimine; C492324:poly(epsilon-caprolactone)-poly(oxyethylene)-poly(epsilon-caprolactone); C005499:honokiol; D000498:Allyl Compounds; D010636:Phenols