Anti-inflammatory activity of hydroxydihydrocarvone.
Hydroxydihydrocarvone (HC) is a synthetic intermediate obtained by hydration of the natural compound (R)-(-)-carvone. The aim of the present study was to investigate the possible anti-inflammatory activity of orally administered HC. Toxicity, motor coordination, tail immersion test, as well as carrageenan-induced paw edema and myeloperoxidase (MPO) activity or peritonitis were all evaluated in rodents. HC was force-fed to the animals 1 h before the stimulus. The lethal dose 50% (LD50) of orally administered HC was 1259 mg/kg. No changes in motor coordination were recorded in HC-treated mice in the rotarod test. The time of response to the thermoceptive stimulus in the tail immersion test was longer in HC-treated animals (50, 100, and 200 mg/kg) than in the vehicle-treated group. HC also significantly decreased the area under curve of carrageenan-induced rat paw edema at 100 and 200 mg/kg and MPO activity at 200 mg/kg. Carrageenan-induced neutrophil recruitment to the peritoneal cavity was significantly reduced by HC at doses of 100 or 200 mg/kg, but not 50 mg/kg. These findings demonstrate that orally administered HC exerts antinociceptive and anti-inflammatory activities in rats and mice.
Departamento de Fisiologia, Universidade Federal de Sergipe, CEP 49100-000, Aracaju, Sergipe, Brazil. damiao_desousa@yahoo.com.br
Damiao P
DP
de Sousa
Enilton A
EA
Camargo
Fernando S
FS
Oliveira
Reinaldo N
RN
de Almeida
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology* / Q000627:therapeutic use / Q000633:toxicity; D002351:Carrageenan / Q000494:pharmacology; D054441:Cell Migration Assays, Leukocyte; D000081005:Cyclohexane Monoterpenes; D004487:Edema / Q000188:drug therapy; D007928:Lethal Dose 50; D008297:Male; D051379:Mice; D039821:Monoterpenes / Q000494:pharmacology* / Q000627:therapeutic use / Q000633:toxicity; D010537:Peritoneum / Q000187:drug effects / Q000502:physiology; D009195:Peroxidase / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
D000893:Anti-Inflammatory Agents; D000081005:Cyclohexane Monoterpenes; D039821:Monoterpenes; C006923:carvone; D002351:Carrageenan; D009195:Peroxidase