Towards clinical proteomics analysis.
To understand accurate protein dynamics, a highly reproducible proteomics analytical method is required. The acquired thus knowledge will lead to the diagnosis, treatment and protection against diseases. This review deals with proteomics analysis from a view of sample pre-treatment, sensitivity and reproducibility of the methods, including two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), liquid chromatography-tandem mass spectrometry (LC-MS/MS). and fluorogenic derivatization (FD)-LC-MS/MS.
Reseach Institute of Pharmaceutical Sciences, Musashino University, Nishitokyo-shi, Tokyo 202-8585, Japan. k-imai@musashino-u.ac.jp
Kazuhiro
K
Imai
Akiyo
A
Koshiyama
Katsunori
K
Nakata
D002853:Chromatography, Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D025202:Molecular Diagnostic Techniques; D040901:Proteomics; D053719:Tandem Mass Spectrometry