Placental alkaline phosphatase isoenzyme in quality control materials may be a source of variability in alkaline phosphatase activity.
OBJECTIVE
To determine the cause of discrepancies in the alkaline phosphatase (ALP) activities of quality control (QC) materials in two different analyzers using IFCC method.


METHODS
ALP activities of patients' samples and QC materials (QC1 and QC2 from Bio-Rad) measured using TBA-200FR and Synchron LX-20 analyzers were compared and isoenzyme electrophoresis was done. Fractional mixing of bone or liver ALP with placental ALP was performed. ALP activities of QC materials were measured in TBA-200FR with pH-modified reagents.


RESULTS
ALP activities of QC materials were significantly lower in TBA-200FR than in LX-20. Placental ALP comprised 57% of QC1 and 95% of QC2. Higher placental ALP proportion in the mixture with bone or liver ALP resulted in lower ALP activities in TBA-200FR. The discrepancy in ALPs of QC materials decreased when measured with pH-modified reagents.


CONCLUSIONS
Placental ALP in QC materials and differences in reagents' pH caused the discrepancy in ALP activities.
Department of Laboratory Medicine, University of Ulsan College of Medicine and Asan Medical Center, Seoul, Republic of Korea.
Eunsin
E
Bae
Hee-Jung
HJ
Chung
Sollip
S
Kim
Woochang
W
Lee
Sail
S
Chun
Won-Ki
WK
Min
D000469:Alkaline Phosphatase; D001842:Bone and Bones; D006801:Humans; D011786:Quality Control
D000469:Alkaline Phosphatase