[The prevalence of Th17 cells in patients with acute myeloid leukemia].
OBJECTIVE
To investigate the proportion of Th17 cells in the peripheral blood of the patients with acute myeloid leukemia (AML) and evaluate the potential association of Th17 cells with AML.


METHODS
The cytokines IL-17 and TGF-β1 in the peripheral blood of AML patients before therapy (group 1), AML patients in complete remission (AML-CR, group 2) and healthy donors (group 3) were measured by enzyme-linked immunosorbent assay (ELISA). The proportion of Th17 cells of each group was evaluated by flow cytometry. The level of IL-17 mRNA of each group was examined by reverse transcription-PCR (RT-PCR).


RESULTS
The percentage of Th17 cells and the level of IL-17, IL-17 mRNA in group 1 \[(10.502 ± 1.071) ng/L, (0.935 ± 0.140)% and 0.262 ± 0.510\] and group 2 \[(11.345 ± 0.987) ng/L, (1.091 ± 0.159)% and 0.307 ± 0.031\] was significantly lower than that in group 3 \[(16.852 ± 1.198) ng/L, (2.586 ± 0.235)% and 0.501 ± 0.060\]. The percentage of Th17 cells and the level of IL-17, IL-17 mRNA in group 1 was lower than that in the group 2. But the level of TGF-β1 in the group 1 (29.963 ± 1.588) ng/L and the group 2 (25.163 ± 1.848) ng/L was significantly higher than that in group 3 (13.366 ± 1.565) ng/L. However, the level of TGF-β1 in the group 3 was higher than that of the group 2.


CONCLUSIONS
Th17 cells might be negatively correlated with the AML development. The overexpression of TGF-β1 in AML patients might suppress the differentiation of Th17 cells.
Department of Hematology, the Second Affiliated Hospital of Lanzhou University, Lanzhou 730000, China.
Zhi-Guo
ZG
Fan
Lian-Sheng
LS
Zhang
Ye
Y
Chai
Peng-Yun
PY
Zeng
Chong-Yang
CY
Wu
D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D020381:Interleukin-17; D015470:Leukemia, Myeloid, Acute; D015995:Prevalence; D058504:Th17 Cells; D053773:Transforming Growth Factor beta1
D020381:Interleukin-17; D053773:Transforming Growth Factor beta1