Specific monoclonal antibody against the nuclear pore complex protein, Nup96.
Nup96 is a component of the Nup107-160 complex, the largest subunit of the nuclear pore complex. Nup96 is generated as a precursor protein with Nup98. However, the mechanism by which Nup96 contributes to cell function is not clear. We report here on the preparation of a monoclonal antibody (MAb) directed against mouse Nup96. The antibody was produced by the hybridization of mouse myeloma cells with lymph node cells from an immunized rat. The antibody, MAb 4H5, specifically recognized Nup96, as evidenced by immunoblotting using the whole cell lysates. In immunostaining using MAb 4H5, a nuclear rim staining pattern was observed. This antibody will be useful in immunoblotting and immunolocalization experiments, as well as further analyses of the biological function and cellular dynamics of this protein.
Biomolecular Dynamics Group, Graduate School of Frontier Biosciences, Osaka University, Osaka, Japan.
Chiaki
C
Mizuguchi
Masahiro
M
Oka
Tetsuji
T
Moriyama
Taro
T
Tachibana
Yoshihiro
Y
Yoneda
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli; D005455:Fluorescent Antibody Technique; D006825:Hybridomas / Q000276:immunology*; D015151:Immunoblotting; D051379:Mice; D041681:NIH 3T3 Cells; D028861:Nuclear Pore Complex Proteins / Q000276:immunology*; D010957:Plasmids / Q000235:genetics; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D051381:Rats; D011994:Recombinant Proteins / Q000276:immunology / Q000302:isolation & purification
D000911:Antibodies, Monoclonal; D028861:Nuclear Pore Complex Proteins; D034741:RNA, Small Interfering; D011994:Recombinant Proteins; C119239:nuclear pore complex protein 96