Anther and stigma morphology in mirror-image flowers of Chamaecrista chamaecristoides (Fabaceae): implications for buzz pollination.
Enantiostyly (mirror-image flowers) is usually associated with buzz pollination. In buzz-pollinated flowers, pollen is released through terminal pores after bees vibrate the stamens. Several studies have evaluated the function of 'buzzing' in pollen release, but less attention has been paid to the effect of buzzing on pollen capture and deposition on stigmas. Evaluating the mechanism of pollen dispersion in buzz-pollinated flowers is important because it may affect mating patterns and reproductive success. In this study, we analysed the morphology of sexual organs (anther and stigma) using electron microscopy, and determined the relationship between sexual organ structure and pollen capture function through experimental manipulations of buzz-pollinated flowers of Chamaecrista chamaecristoides, as well as vibration frequencies on floral visitors. Pollen release occurs through two terminal pores at the tip of the stamens. However, unlike most angiosperms that have their stigmatic surface exposed, C. chamaecristoides presents a stigmatic surface inside a cavity covered by trichomes. Experimental manipulations showed that effective fertilisation is only achieved when the style is vibrated, suggesting that buzzing is not only important for pollen release but also for pollen capture and deposition on the stigma. This result, in addition to vibration frequency analysis, suggests that although all floral visitors buzz flowers only those that buzz at higher frequencies achieve effective fertilisation. The anatomical features of sexual organs in flowers of C. chamaecristoides demonstrate that this species possesses a highly specialised, elaborate morphology, with both genders selected for traits that promote buzz pollination.
Departamento de Ecología Funcional, Instituto de Ecología, Xalapa, Veracruz, México. gea13@pitt.edu
G
G
Arceo-Gómez
M L
ML
Martínez
V
V
Parra-Tabla
J G
JG
García-Franco
D000818:Animals; D001516:Bees / Q000502:physiology; D031288:Chamaecrista / Q000033:anatomy & histology / Q000235:genetics*; D035264:Flowers / Q000033:anatomy & histology / Q000235:genetics* / Q000648:ultrastructure; D019684:Magnoliopsida; D008800:Mexico; D011058:Pollen / Q000033:anatomy & histology; D054817:Pollination; D012098:Reproduction; D014796:Visual Perception