A feedback circuit between transcriptional activation and self-destruction of Gcn4 separates its metabolic and morphogenic response in diploid yeasts.
The basic zipper Gcn4 protein activates transcription in the yeast Saccharomyces cerevisiae in response to amino acid starvation. This includes numerous metabolic genes of amino acid or purine biosynthesis and the developmental cell-surface flocculin gene FLO11, which is required for diploid pseudohyphae formation and for adhesion upon nutrient starvation. We separated the metabolic from the developmental response by screening for GCN4 alleles that allow growth during amino acid starvation but are impaired in adhesion and are unable to form pseudohyphae. The identified Gcn4(L267S) variant carries an amino acid substitution in the third of the four conserved leucines of the zipper dimerization domain. This mutation abolished FLO11 expression and results in reduced but sufficient transcriptional activity for amino acid biosynthetic genes. The Leu267Ser substitution impairs Gcn4 homodimer formation and is a significantly more stable protein than the wild-type protein. A helix-breaker substitution in Leu253 results in a transcriptionally inactive but highly stable protein variant. This is due to a feedback circuit between transcriptional activity of Gcn4 and its own stability, which depends on the Gcn4-controlled cyclin PCL5. Gcn4(L253G) reduces the expression of Pcl5 and therefore reduces its own degradation. This self-controlled buffer system to restrict transcriptional activity results in a reciprocal correlation between Gcn4 transcriptional activity and protein stability.
Institute of Microbiology and Genetics, Georg-August-Universität, Grisebachstr. 8, D-37077 Göttingen, Germany.
Britta
B
Herzog
Katrin
K
Streckfuss-Bömeke
Gerhard H
GH
Braus
D019943:Amino Acid Substitution; D050976:Basic-Leucine Zipper Transcription Factors / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D016213:Cyclins / Q000235:genetics; D019281:Dimerization; D004171:Diploidy; D025461:Feedback, Physiological; D018507:Gene Expression Regulation, Developmental; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D007763:Lac Operon; D016350:Leucine Zippers / Q000235:genetics; D008562:Membrane Glycoproteins / Q000235:genetics; D008954:Models, Biological; D008958:Models, Molecular; D050505:Mutant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D055550:Protein Stability; D020836:Protein Structure, Quaternary; D017434:Protein Structure, Tertiary; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D015533:Transcriptional Activation
D050976:Basic-Leucine Zipper Transcription Factors; D016213:Cyclins; C110690:FLO11 protein, S cerevisiae; C501391:GCN4 protein, S cerevisiae; D008562:Membrane Glycoproteins; D050505:Mutant Proteins; C461492:PCL5 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins