[Direct interaction between BAG5 protein and Parkin protein].
OBJECTIVE
To explore the interaction between BCL2-associated athanogene 5 (BAG5) and Parkin protein,and the regulatory mechanism of BAG5 protein on the level of Parkin protein.


METHODS
We performed GST pull-down assay to identify which domain of PINK1 interacted with Parkin, and generated different deletions of BAG5 to identify the domains. Chase time experiment was done to determine the effect of co-regulation of BAG5 protein on the ubiquitination. We further examined the possible interaction between Parkin and PINK1 in 293A cells by co-immunoprecipitation method.


RESULTS
BAG5 directly interacted with the Parkin, and all the 4 BAG domains interacted with the Parkin. BAG5 stabilized the Parkin by interfering its degradation via the ubiquitin-mediated proteasomal pathway.


CONCLUSIONS
BAG5 directly interacts with the Parkin, and BAG5 stabilizes the Parkin via the ubiquitin-mediated proteasomal pathway.
Department of Neurology, Xiangya Hospital, Central South University, Changsha 410008, China.
Xuejing
X
Wang
Jifeng
J
Guo
Hong
H
Jiang
Lu
L
Shen
Beisha
B
Tang
D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism*; D006801:Humans; D010300:Parkinson Disease / Q000378:metabolism; D011485:Protein Binding; D044767:Ubiquitin-Protein Ligases / Q000378:metabolism*; D054875:Ubiquitination
D048868:Adaptor Proteins, Signal Transducing; C496515:BAG5 protein, human; D044767:Ubiquitin-Protein Ligases; C111567:parkin protein