A novel function of the mitochondrial transcription factor Mtf1 in fission yeast; Mtf1 regulates the nuclear transcription of srk1.
In eukaryotic cells, Mtf1 and its homologues function as mitochondrial transcription factors for the mitochondrial RNA polymerase in the mitochondrion. Here we show that in fission yeast Mtf1 exerts a non-mitochondrial function as a nuclear factor that regulates transcription of srk1, which is a kinase involved in the stress response and cell cycle progression. We first found Mtf1 expression in the nucleus. A ChIP-chip approach identified srk1 as a putative Mtf1 target gene. Over expression of Mtf1 induced transcription of the srk1 gene and Mtf1 deletion led to a reduction in transcription of the srk1 gene in vivo. Mtf1 overexpression causes cell elongation in a srk1 dependent manner. Mtf1 overexpression can cause cytoplasmic accumulation of Cdc25. We also provide biochemical evidence that Mtf1 binds to the upstream sequence of srk1. This is the first evidence that a mitochondrial transcription factor Mtf1 can regulate a nuclear gene. Mtf1 may also have a role in cell cycle progression.
Institute of Biomedical Science, Fudan University, Yi Xue Yuan Road 138, 200032 Shanghai, China.
Wenxia
W
Sun
Zhe
Z
Wang
Hengyi
H
Jiang
Jing
J
Zhang
Jürg
J
Bähler
Dongrong
D
Chen
Alastair I H
AI
Murchie
D002467:Cell Nucleus / Q000737:chemistry / Q000235:genetics*; D047369:Chromatin Immunoprecipitation; D003593:Cytoplasm / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000032:analysis / Q000378:metabolism / Q000502:physiology*; D015966:Gene Expression Regulation, Fungal; D024101:Mitochondrial Proteins / Q000032:analysis / Q000378:metabolism / Q000502:physiology*; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D012568:Schizosaccharomyces / Q000166:cytology / Q000235:genetics / Q000378:metabolism; D029702:Schizosaccharomyces pombe Proteins / Q000032:analysis / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D014157:Transcription Factors / Q000032:analysis / Q000378:metabolism / Q000502:physiology*; D014158:Transcription, Genetic; D020687:cdc25 Phosphatases / Q000378:metabolism; D000097809:Transcription Factor MTF-1
D004268:DNA-Binding Proteins; D024101:Mitochondrial Proteins; D020928:Mitogen-Activated Protein Kinases; D029702:Schizosaccharomyces pombe Proteins; D014157:Transcription Factors; D020687:cdc25 Phosphatases; C559090:Mtf1 protein, S pombe; D000097809:Transcription Factor MTF-1; C422562:mitochondrial transcription factor A; C499611:SRK1 protein, S pombe