Intra-peritoneal application of catechins and EGCG as in vivo inhibitors of ozone-induced oxidative stress.
Oxidative stress is considered as a prominent feature of many acute and chronic diseases as well as of the normal aging process. We examined the effects of intra-peritoneal administration of catechins and EGCG as in vivo inhibitors of oxidative stress induced by ozone administration in two groups of Wistar rats. The first group was treated by intra-peritoneal administration of catechins and EGCG after the administration of ozone and the second group was pretreated by intra-peritoneal administration of catechins and EGCG prior to ozone administration. We determined in blood the activity of the enzymes superoxide dismutase and glutathione peroxidase, total antioxidant capacity, levels of copper and zinc and in urine malonaldehyde contents. Ozone administration resulted in significant reduction of glutathione peroxidase activity, plasma zinc levels and plasma and Red Blood Cells antioxidant capacity. Catechins and EGCG upregulate superoxide dismutase activity and maintain plasma and Red Blood Cells antioxidant capacity. Malonaldehyde levels at the end of the study were significantly increased only in the first group. Our data demonstrate that treatment with catechins and EGCG cannot reverse or prevent the effects of oxidative stress although some modulation occurs.
Laboratory of Physiology, Faculty of Medicine, University of Ioannina, Ioannina 45110, Greece. simosyannis@gmail.com
Y
Y
Simos
S
S
Karkabounas
I
I
Verginadis
P
P
Charalampidis
D
D
Filiou
K
K
Charalabopoulos
I
I
Zioris
V
V
Kalfakakou
A
A
Evangellou
D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology*; D002392:Catechin / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology*; D003300:Copper / Q000097:blood / Q000652:urine; D005260:Female; D005979:Glutathione Peroxidase / Q000097:blood / Q000187:drug effects; D007274:Injections, Intraperitoneal; D015227:Lipid Peroxidation / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D010126:Ozone / Q000009:adverse effects / Q000493:pharmacokinetics; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000097:blood / Q000187:drug effects; D015032:Zinc / Q000097:blood / Q000652:urine
D000975:Antioxidants; D010126:Ozone; D003300:Copper; D002392:Catechin; C045651:epigallocatechin gallate; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D015032:Zinc