Small subunit ribosomal RNA and mitochondrial cytochrome c oxidase subunit 1 gene sequences of 21 strains of the parasitic scuticociliate Miamiensis avidus (Ciliophora, Scuticociliatia).
The scuticociliate Miamiensis avidus is a histophagous parasite that causes high mortality in cultured marine fishes. Small subunit ribosomal RNA (SSU rRNA) and mitochondrial cytochrome c oxidase subunit 1 (cox1) genes were analyzed for 21 strains of M. avidus isolated from diseased olive flounder (Paralichthys olivaceus), ridged-eye flounder (Pleuronichthys cornutus), and spotted knifejaw (Oplegnathus fasciatus) in Korea and Japan (collected in 2003-2007). Analysis of SSU rRNA gene sequences (1,759 bp) indicates they are very conserved with less than 0.17% (3 nucleotides) differences suggesting that SSU rRNA are useful to identify M. avidus; however, the cox1 gene (900 bp) has higher variations with intraspecific divergences up to 5.67% (51 nucleotides). A distance tree of cox1 gene sequences based on a neighbor-joining analysis can separate 21 strains into five cox1 types (two heterogeneous clusters and three individual branches). The cox1-type matches with serotype of strains but do not reflect geographical origins, host species, or pathogenicity.
Department of Aqualife Medicine, Chonnam National University, Chonnam, 550-749, South Korea.
Sung-Ju
SJ
Jung
Eun-Young
EY
Im
Michaela C
MC
Strüder-Kypke
Shin-Ichi
S
Kitamura
Patrick T K
PT
Woo
D000818:Animals; D016000:Cluster Analysis; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D003576:Electron Transport Complex IV / Q000235:genetics*; D005393:Fish Diseases / Q000469:parasitology*; D005412:Flatfishes / Q000469:parasitology; D007564:Japan; D008969:Molecular Sequence Data; D016802:Oligohymenophorea / Q000235:genetics*; D010473:Perciformes / Q000469:parasitology; D010802:Phylogeny; D011110:Polymorphism, Genetic; D016053:RNA, Protozoan / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics*; D056910:Republic of Korea; D017422:Sequence Analysis, DNA
D016054:DNA, Protozoan; D004275:DNA, Ribosomal; D016053:RNA, Protozoan; D012337:RNA, Ribosomal, 18S; D003576:Electron Transport Complex IV