Monitoring glutathione homeostasis in nanoparticle-treated hepatocytes.
This chapter describes a method for the analysis of human hepatocarcinoma cells (Hep G2 cells) for reduced and oxidized glutathione, following treatment with nanoparticle formulations. Glutathione is a tripeptide (L-γ-glutamyl-L-cysteinyl-glycine) present intracellularly in millimolar concentrations and one of the primary cellular antioxidant defenses against oxidative stress. An increase in the relative amount of oxidized to reduced glutathione may be indicative of oxidative stress, while a decrease in the overall glutathione pool may be indicative of conjugative metabolism or impaired synthesis. The method presented in this chapter utilizes a colorimetric method for detection of reduced and oxidized glutathione.
Nanotechnology Characterization Laboratory, Advanced Technology Program, SAIC-Frederick, Inc., National Cancer Institute at Frederick, Frederick, MD, USA.
Timothy M
TM
Potter
Barry W
BW
Neun
Stephan T
ST
Stern
D000975:Antioxidants / Q000378:metabolism; D002478:Cells, Cultured; D003124:Colorimetry / Q000379:methods; D005978:Glutathione / Q000378:metabolism*; D019803:Glutathione Disulfide / Q000378:metabolism; D056945:Hep G2 Cells; D022781:Hepatocytes / Q000187:drug effects* / Q000378:metabolism; D006706:Homeostasis / Q000187:drug effects; D006801:Humans; D053758:Nanoparticles / Q000633:toxicity*; D010084:Oxidation-Reduction; D018384:Oxidative Stress
D000975:Antioxidants; D005978:Glutathione; D019803:Glutathione Disulfide