Oral squamous cell carcinoma versus oral verrucous carcinoma: an approach to cellular proliferation and negative relation to human papillomavirus (HPV).
Human papillomavirus (HPV) has been cited as a possible initiating agent in the pathogenesis of oral cancer. However, the literature tends to be both controversial and inconclusive about the prevalence of HPV and its potential for proliferation in oral squamous cell carcinoma (SCC). The aim of this study was to investigate the cellular proliferation and the presence of HPV in SCC and verrucous carcinoma (VC). Forty-seven samples of SCC were selected and divided into three groups: 39 SCC, 8 VC, and 9 of normal mucosa (control-CT). Quantitative analyses of all groups showed a greater expression of PCNA, followed by Ki-67 and cyclin B1. A significant difference was observed in cyclin B1 expression in the SCC group compared with VC. PCNA, Ki-67, and cyclin B1 were statistically significant when comparing the SCC and CT groups. However, when SCC and VC were compared, there was no difference in Ki-67 expression. Our results showed that only cyclin B1 had an association with histological grade, and that poorly differentiated tumors presented a higher expression of cyclin B1. Therefore, considerable differences in the cellular proliferation between SCC and VC were observed, and no correlation with HPV was established, since all samples were negative for HPV.
Department of Stomatology (Oral Pathology), Dental School, Federal University of Goiás, Goiás, Brazil.
José Vieira
JV
de Spíndula-Filho
Aparecido Divino
AD
da Cruz
Angélica Ferreira
AF
Oton-Leite
Aline Carvalho
AC
Batista
Cláudio Rodrigues
CR
Leles
Rita
R
de Cássia Gonçalves Alencar
Vera Aparecida
VA
Saddi
Elismauro Francisco
EF
Mendonça
D000368:Aged; D052685:Alphapapillomavirus / Q000302:isolation & purification*; D001938:Brazil; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000473:pathology* / Q000821:virology*; D018289:Carcinoma, Verrucous / Q000378:metabolism / Q000473:pathology* / Q000821:virology*; D016022:Case-Control Studies; D049109:Cell Proliferation; D056744:Cyclin B1 / Q000378:metabolism; D005260:Female; D006801:Humans; D019394:Ki-67 Antigen / Q000378:metabolism; D008297:Male; D009062:Mouth Neoplasms / Q000378:metabolism / Q000473:pathology* / Q000821:virology*; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism
D056744:Cyclin B1; D019394:Ki-67 Antigen; D018809:Proliferating Cell Nuclear Antigen