[Effects of xbp-1 gene silencing on bortezomib-induced apoptosis in human multiple myeloma cells].
This study was purposed to investigate the effect of xbp-1 gene silencing on bortezomib-induced apoptosis in multiple myeloma cell line NCI-H929 (H929). After xbp-1 gene expression was interfered by small hairpin RNA, the cell apoptosis was assayed by flow cytometry with Annexin V-FITC/PI staining, and the expression level of XBP-1 protein was detected by Western blot. The results showed that XBP-1 protein level of H929 cells was inhibited effectively by the PLL3.7 lentiviral vector mediated expression xbp-1 shRNA. The apoptosis rate was significantly higher in xbp-1 shRNA-expressing cells than in untreated control group [(10.13±0.61)% vs (2.5±0.2)%, p<0.05]. After treatment with bortezomib, the apoptosis rate of XBP-1 protein functionally deficient H929 cells was significantly higher than those in vector control group [(45.07±1)% vs (19.53±0.8)%, p<0.05]. It is concluded that xbp-1 gene silencing can significantly enhance the pro-apoptotic activity of bortezomib in multiple myeloma cells.
Department of Hematology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, Shaanxi Province, China.
Yang
Y
Yang
Hong-Juan
HJ
Dong
Guang-Xun
GX
Gao
Yi-Wei
YW
Wang
Hong-Tao
HT
Gu
Mi-Mi
MM
Shu
Hua-Feng
HF
Zhu
Xie-Qun
XQ
Chen
D017209:Apoptosis / Q000187:drug effects*; D001897:Boronic Acids / Q000494:pharmacology*; D000069286:Bortezomib; D045744:Cell Line, Tumor; D004268:DNA-Binding Proteins / Q000235:genetics*; D020868:Gene Silencing; D006801:Humans; D009101:Multiple Myeloma / Q000235:genetics*; D011719:Pyrazines / Q000494:pharmacology*; D034741:RNA, Small Interfering / Q000235:genetics; D000071701:Regulatory Factor X Transcription Factors; D014157:Transcription Factors / Q000235:genetics*; D000071717:X-Box Binding Protein 1
D001897:Boronic Acids; D004268:DNA-Binding Proteins; D011719:Pyrazines; D034741:RNA, Small Interfering; D000071701:Regulatory Factor X Transcription Factors; D014157:Transcription Factors; D000071717:X-Box Binding Protein 1; C000605543:XBP1 protein, human; D000069286:Bortezomib