Amphiphilic polysaccharide nanoballs: a new building block for nanogel biomedical engineering and artificial chaperones.
Enzymatically synthesized glycogen (ESG), a highly branched (1→4)(1→6)-linked α-glucan, is a new monodisperse spherical hyperbranched nanoparticle (molecular weight, 10(6)-10(7); diameter, 20-30 nm), polysaccharide nanoball. Amphiphilic ESG nanoballs were synthesized by introducing a cholesterol group to enzymatically synthesized glycogen (CHESG). CHESG assembled into a structure containing a few molecules to form cluster nanogels (approximately 35 nm in diameter) in water. The cluster nanogels were dissociated by the addition of cyclodextrin (CD) to form a supramolecular CHESG-CD nanocomplex due to complexation with the cholesterol group and CD. The CHESG nanogel showed high capacity for complexation with proteins, and the CHESG-CD nanocomplex showed high chaperone-like activity for thermal stabilization of enzymes. CHESG has great potential to become a new building block for nanogel biomedical engineering and to act as an artificial chaperone for protein engineering.
Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda-ku, Tokyo 101-0062, Japan.
Haruko
H
Takahashi
Shin-Ichi
S
Sawada
Kazunari
K
Akiyoshi
D000818:Animals; D001698:Biomedical Engineering / Q000379:methods*; D040761:Biomimetic Materials / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism*; D002236:Carbohydrate Conformation; D002417:Cattle; D002784:Cholesterol / Q000737:chemistry; D003505:Cyclodextrins / Q000378:metabolism; D005782:Gels; D006003:Glycogen / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism*; D057927:Hydrophobic and Hydrophilic Interactions; D008958:Models, Molecular; D018832:Molecular Chaperones / Q000378:metabolism*; D053758:Nanoparticles / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000378:metabolism; D014867:Water / Q000737:chemistry
D003505:Cyclodextrins; D005782:Gels; D018832:Molecular Chaperones; D014867:Water; D012710:Serum Albumin, Bovine; D006003:Glycogen; D002784:Cholesterol