Basic-helix-loop-helix transcription factor DEC2 constitutes negative feedback loop in IFN-β-mediated inflammatory responses in human mesangial cells.
Differentiated embryo-chondrocyte 2 (DEC2), a basic-helix-loop-helix transcriptional factor, is involved in various biological reactions by regulating the expression of its target genes. In the present study, we demonstrated DEC2 expression in response to the treatment with polyinosinic-polycytidylic acid (poly IC), an authentic double-stranded RNA, in cultured human mesangial cells. RNA interference against DEC2 enhanced the poly IC-induced expression of IFN-β and its downstream genes, retinoic acid-inducible gene-I (RIG-I) and CCL5. Knockdown of TLR3 abolished the poly IC-induced DEC2 expression. DEC2 expression may constitute a negative feedback system for the IFN-β/RIG-I/CCL5 pathway in the glomerulus, which may play a role in controlling protracted inflammatory reactions in the kidney.
Department of Vascular Biology, Hirosaki University Graduate School of Medicine, 5 Zaifu-cho, Hirosaki 036-8562, Japan. timaizum@cc.hirosaki-u.ac.jp
Tadaatsu
T
Imaizumi
Fuyuki
F
Sato
Hiroshi
H
Tanaka
Tomoh
T
Matsumiya
Hidemi
H
Yoshida
Tomomi
T
Yashiro-Aizawa
Kazushi
K
Tsuruga
Ryo
R
Hayakari
Hiroshi
H
Kijima
Kei
K
Satoh
D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000276:immunology*; D002478:Cells, Cultured; D018946:Chemokine CCL5 / Q000276:immunology; D000071457:DEAD Box Protein 58; D053487:DEAD-box RNA Helicases / Q000276:immunology; D006801:Humans; D007249:Inflammation / Q000276:immunology; D016899:Interferon-beta / Q000276:immunology*; D050527:Mesangial Cells / Q000276:immunology*; D011070:Poly I-C / Q000276:immunology; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D011971:Receptors, Immunologic; D051196:Toll-Like Receptor 3 / Q000276:immunology
D051792:Basic Helix-Loop-Helix Proteins; D018946:Chemokine CCL5; D000071457:DEAD Box Protein 58; D053487:DEAD-box RNA Helicases; D016899:Interferon-beta; D011070:Poly I-C; D034741:RNA, Small Interfering; D011971:Receptors, Immunologic; D051196:Toll-Like Receptor 3; C422085:BHLHE41 protein, human; C483024:CCL5 protein, human; C495344:TLR3 protein, human; C452820:RIGI protein, human