Chemical and biological investigations of Delonix regia (Bojer ex Hook.) Raf.
In this study, five compounds, lupeol (1), epilupeol (2), β-sitosterol (3), stigmasterol (4) and p-methoxybenzaldehyde (5) were isolated from the petroleum ether and dichloromethane fractions of a methanolic extract of the stem bark of Delonix regia. Antimicrobial screening of the different extracts (15 μg mm-2) was conducted by the disc diffusion method. The zones of inhibition demonstrated by the petroleum ether, carbon tetrachloride and dichloromethane fractions ranged from 9-14 mm, 11-13 mm and 9-20 mm, respectively, compared to kanamycin standard with the zone of inhibition of 20-25 mm. In brine shrimp lethality bioassay, the carbon tetrachloride soluble materials demonstrated the highest toxicity with LC50 of 0.83 μg mL-1, while petroleum ether and dichloromethane soluble partitionates of the methanolic extract revealed LC50 of 14.94 and 3.29 μg mL-1, respectively, in comparison with standard vincristine sulphate with LC50 of 0.812 μg mL-1. This is the first report on compounds separation from D. regia, their antimicrobial activity and cytotoxicity.
Department of Clinical Pharmacy and Pharmacology, Faculty of Pharmacy, University of Dhaka, Dhaka-1000, Bangladesh.
Israt
I
Jahan
Mohammad S
MS
Rahman
Mohammed Z
MZ
Rahman
Mohammad A
MA
Kaisar
Mohammad S
MS
Islam
Abdul
A
Wahab
Mohammad A
MA
Rashid
D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D001547:Benzaldehydes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D007887:Fabaceae / Q000737:chemistry*; D007612:Kanamycin / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D053978:Pentacyclic Triterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D012855:Sitosterols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D013265:Stigmasterol / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D000094042:Lupanes
D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D001547:Benzaldehydes; D007612:Kanamycin; D053978:Pentacyclic Triterpenes; D010936:Plant Extracts; D012855:Sitosterols; D013265:Stigmasterol; C025473:gamma-sitosterol; C024896:4-anisaldehyde; C010480:lupeol; D000094042:Lupanes