A sensitive and selective HPLC method for estimation of lamotrigine in human plasma and saliva: application to plasma-saliva correlation in epileptic patients.
A sensitive and selective high performance liquid chromatographic (HPLC) method was developed and validated for estimation of lamotrigine (CAS 84057-84-1) in human plasma and saliva. The chromatographic separation was achieved with a reversed phase column and a mobile phase consisting of acetonitrile and 20 mM ammonium acetate buffer pH 6.5 (30:70) with a flow rate of 1 mL/min. The calibration curve was linear within the working range for both plasma and saliva. The validated method has been successfully applied for a study of lamotrigine in human plasma and saliva to establish the correlation between these two matrices. A scatter plot of plasma versus salivary lamotrigine concentrations showed a gold linear relationship between them (Pearson correlation coefficient, r = 0.6832, p < 0.001).
Manipal College of Pharmaceutical Sciences, Manipal University, Manipal, India. msvragavrajan@gmail.com
Surulivel Rajan
SR
Mallayasamy
Karthik
K
Arumugamn
Tarun
T
Jain
Thiyagu
T
Rajakannan
Krishnamurthy
K
Bhat
Padma
P
Gurumadhavrao
Ramakrishna
R
Devarakonda
D000927:Anticonvulsants / Q000032:analysis* / Q000097:blood / Q000493:pharmacokinetics; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D004827:Epilepsy / Q000378:metabolism*; D006801:Humans; D007202:Indicators and Reagents; D000077213:Lamotrigine; D010672:Phenytoin / Q000032:analysis / Q000097:blood; D012015:Reference Standards; D015203:Reproducibility of Results; D012463:Saliva / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D014227:Triazines / Q000032:analysis* / Q000097:blood / Q000493:pharmacokinetics
D000927:Anticonvulsants; D007202:Indicators and Reagents; D014227:Triazines; D010672:Phenytoin; D000077213:Lamotrigine