Cardiac and vascular gene profiles in an animal model of takotsubo cardiomyopathy.
We investigated cardiac and vascular gene profiles in response to immobilization stress (IMO) in rats, an animal model of emotional stress-induced takotsubo cardiomyopathy using microarray analysis, followed by re-confirmation with real-time reverse transcription-polymerase chain reaction. Expression levels of the identified genes were further estimated by pretreatment with an α1-adrenoceptor blocker and/or a β1-adrenoceptor blocker. In response to IMO, expression of 46 genes was significantly altered in the heart and that of 49 genes was significantly altered in the aorta. Pathway analysis with DAVID Bioinformatics Resources indicated that regulation of transcription and response to endogenous stimulation were the top two scoring pathways. Altered expression of cardiac genes was blunted by pretreatment with a β1-adrenoceptor blocker or α1 + β1-adrenoceptor blockers. In contrast, that of aortic genes was blunted by pretreatment with an α1-adrenoceptor blocker or α1 + β1-adrenoceptor blockers. Activation of α1-adrenoceptor in the blood vessels or activation of β1-adrenoceptors in the heart were mainly responsible for emotional stress-induced alteration of cardiac and vascular gene profiles.
Department of Anatomy and Cell Biology, Wakayama Medical University School of Medicine, 811-1 Kimiidera, Wakayama, 641-8509, Japan. tueyama@wakayama-med.ac.jp
Takashi
T
Ueyama
Yuta
Y
Yamamoto
Kazuki
K
Ueda
Tetsuya
T
Kawabe
Takuzo
T
Hano
Takao
T
Ito
Yoshihiro
Y
Tsuruo
Masao
M
Ichinose
Ken-ichi
K
Yoshida
D058668:Adrenergic alpha-1 Receptor Antagonists / Q000494:pharmacology; D058671:Adrenergic beta-1 Receptor Antagonists / Q000494:pharmacology; D000704:Analysis of Variance; D000818:Animals; D001011:Aorta / Q000187:drug effects / Q000378:metabolism*; D019295:Computational Biology; D004195:Disease Models, Animal; D004644:Emotions; D020869:Gene Expression Profiling / Q000379:methods; D005786:Gene Expression Regulation; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D051381:Rats; D017208:Rats, Wistar; D012149:Restraint, Physical / Q000523:psychology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013315:Stress, Psychological / Q000150:complications* / Q000235:genetics; D054549:Takotsubo Cardiomyopathy / Q000235:genetics* / Q000523:psychology; D013997:Time Factors; D014158:Transcription, Genetic
D058668:Adrenergic alpha-1 Receptor Antagonists; D058671:Adrenergic beta-1 Receptor Antagonists