Simplified RP-HPLC method for multi-residue analysis of abamectin, emamectin benzoate and ivermectin in rice.
A rapid, reliable and sensitive reverse-phase high-performance liquid chromatography method with fluorescence detection (RP-FLD-HPLC) was developed and validated for simultaneous analysis of the abamectin (ABA), emamectin (EMA) benzoate and ivermectin (IVM) residues in rice. After extraction with acetonitrile/water (2 : 1) with sonication, the avermectin (AVMs) residues were directly derivatised by N-methylimidazole (N-NMIM) and trifluoroacetic anhydride (TFAA) and then analysed on RP-FLD-HPLC. A good linear relationship (r(2 )> 0.99) was obtained for three AVMs ranging from 0.01 to 5 microg ml(-1), i.e. 0.01-5.0 microg g(-1) in rice matrix. The limit of detection (LOD) and the limit of quantification (LOQ) were between 0.001 and 0.002 microg g(-1) and between 0.004 and 0.006 microg g(-1), respectively. Recoveries were from 81.9% to 105.4% and precision less than 12.4%. The proposed method was successfully applied to routine analysis of the AVMs residues in rice.
State Key Laboratory of Pollution Control and Resource Reuse, Center for Hydrosciences Research, Nanjing University, School of the Environment, Nanjing 210093, China. xchxie@yahoo.com.cn
Xianchuan
X
Xie
Shu
S
Gong
Xiaorong
X
Wang
Yinxing
Y
Wu
Li
L
Zhao
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007306:Insecticides / Q000032:analysis*; D007559:Ivermectin / Q000031:analogs & derivatives* / Q000032:analysis*; D057230:Limit of Detection; D012275:Oryza / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
D007306:Insecticides; C048324:abamectin; D007559:Ivermectin; C108024:emamectin benzoate