Novel oligonucleotide carrier as scavenger for reactive oxygen species.
A novel oligonucleotide carrier which can scavenge ROS is described. The synthesized graft polymer is composed of a PEG segment and a TEMPO-containing hydrophobic segment for scavenging ROS. This graft polymer can form a PIC through electrostatic interaction with oligonucleotides such as siRNA. The amount of ROS was monitored by fluorescence measurements using H₂ DCFDA as a probe, and it was confirmed that the ROS level was effectively suppressed. The cellular uptake of PIC containing the fluorescence-labeled oligonucleotide was evaluated by fluorescence microscopy. Delivered siRNA suppressed the expression of the mRNA. The prepared graft copolymer is thus a promising candidate as a novel oligonucleotide carrier which also reduces ROS damage generated by cationic polymer carriers.
Graduate School of Pure and Applied Science, and Tsukuba Research Center for Interdisciplinary Materials Science (TIMS), University of Tsukuba, 1-1-1 Ten-noudai, Tsukuba, Ibaraki 305-8573, Japan.
Yutaka
Y
Ikeda
Rie
R
Suzuki
Toru
T
Yoshitomi
Yukio
Y
Nagasaki
D004337:Drug Carriers / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D016166:Free Radical Scavengers / Q000494:pharmacology*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D056945:Hep G2 Cells; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000235:genetics / Q000378:metabolism; D007477:Ions; D009682:Magnetic Resonance Spectroscopy; D009841:Oligonucleotides / Q000737:chemistry*; D010880:Piperidines / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*; D055672:Static Electricity; D000077022:Survivin
C528764:2,2,6,6-tetramethyl-1-piperidine; C107489:BIRC5 protein, human; D004337:Drug Carriers; D016166:Free Radical Scavengers; D051034:Inhibitor of Apoptosis Proteins; D007477:Ions; D009841:Oligonucleotides; D010880:Piperidines; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D000077022:Survivin; D011092:Polyethylene Glycols