Use of the Capripoxvirus homologue of Vaccinia virus 30 kDa RNA polymerase subunit (RPO30) gene as a novel diagnostic and genotyping target: development of a classical PCR method to differentiate Goat poxvirus from Sheep poxvirus.
Sheep poxvirus (SPPV), Goat poxvirus (GTPV) and Lumpy skin disease virus (LSDV) are Capripoxviruses (CaPVs) responsible for causing severe poxvirus disease in sheep, goats and cattle, respectively. Serological differentiation of CaPVs is not possible and strain identification has relied on the implicitly accepted hypothesis that the viruses show well defined host specificity. However, it is now known that cross infections can occur and authentication of identity based on the host animal species from which the strain was first isolated, is not valid and should be replaced with molecular techniques to allow unequivocal strain differentiation. To identify a diagnostic target for strain genotyping, the CaPV homologue of the Vaccinia virus E4L gene which encodes the 30 kDa DNA-dependent RNA polymerase subunit, RPO30 was analyzed. Forty-six isolates from different hosts and geographical origins were included. Most CaPVs fit into one of the three different groups according to their host origins: the SPPV, the GTPV and the LSDV group. A unique 21-nucleotide deletion was found in all SPPV isolates which was exploited to develop a RPO30-based classical PCR test to differentiate SPPV from GTPV that will allow rapid differential diagnosis of disease during CaPV outbreaks in small ruminants.
Animal Production and Health Laboratory, FAO/IAEA Agriculture & Biotechnology Laboratory, IAEA Laboratories Seibersdorf, International Atomic Energy Agency, Wagramer Strasse 5, P.O. Box 100, A1400 Vienna, Austria.
Charles Euloge
CE
Lamien
Christian
C
Le Goff
Roland
R
Silber
David B
DB
Wallace
Velý
V
Gulyaz
Eeva
E
Tuppurainen
Hafsa
H
Madani
Philippe
P
Caufour
Tajelser
T
Adam
Mehdi
M
El Harrak
Antony George
AG
Luckins
Emmanuel
E
Albina
Adama
A
Diallo
D000595:Amino Acid Sequence; D000818:Animals; D018151:Capripoxvirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D002417:Cattle / Q000821:virology; D002418:Cattle Diseases / Q000175:diagnosis / Q000821:virology; D004279:DNA, Viral / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D005838:Genotype; D015511:Goat Diseases / Q000175:diagnosis / Q000821:virology; D006041:Goats / Q000821:virology; D058507:Host Specificity; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000662:veterinary; D011213:Poxviridae Infections / Q000175:diagnosis* / Q000662:veterinary; D016415:Sequence Alignment; D017384:Sequence Deletion; D012756:Sheep / Q000821:virology; D012757:Sheep Diseases / Q000175:diagnosis / Q000821:virology; D013045:Species Specificity; D014616:Vaccinia virus / Q000235:genetics
D004279:DNA, Viral; D012321:DNA-Directed RNA Polymerases