Validation of RNAi by real time PCR.
Real time PCR is the analytic tool of choice for quantification of gene expression, while RNAi is concerned with downregulation of gene expression. Together, they constitute a powerful approach in any loss of function studies of selective genes. We illustrate here the use of real time PCR to verify luciferase mRNA silencing.
The Bartholin Institute, Copenhagen University Hospital, Copenhagen, Denmark. knud@eln.dk
Knud
K
Josefsen
Ying C
YC
Lee
D052160:Benzothiazoles; D017931:DNA Primers / Q000235:genetics; D003959:Diamines; D015870:Gene Expression; D006367:HeLa Cells; D006801:Humans; D008156:Luciferases / Q000235:genetics; D009930:Organic Chemicals / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods*; D011804:Quinolines; D034622:RNA Interference
D052160:Benzothiazoles; D017931:DNA Primers; D003959:Diamines; D009930:Organic Chemicals; D011804:Quinolines; C098022:SYBR Green I; D008156:Luciferases