High-performance liquid chromatography assay of gnetol in rat serum and application to pre-clinical pharmacokinetic studies.
A method of analysis of gnetol (2,3',5',6-tetrahydroxy-trans-stilbene) in biological fluids is necessary for the study of its kinetics and disposition in plants. A simple high-performance liquid chromatography (HPLC) method was developed for the determination of gnetol in rat serum using a reverse-phase, isocratic system. Separation was achieved using a Phenomenex® Luna C₁₈(2) column with ultraviolet detection at 305 nm. The standard curves were linear ranging from 0.5 to 100 μg/ml. The mean extraction efficiency was >90.5%. Precision of the assay was <14% (R.S.D.), and was within 14% at the limit of quantitation (0.5 μg/ml). Bias of the assay was lower than 15%, and was within 15% at the limit of quantitation. The assay was applied successfully to the study of serum disposition of gnetol in rats.
College of Pharmacy, Department of Pharmaceutical Sciences, Washington State University, Pullman, WA 99164-6534, USA.
Connie M
CM
Remsberg
Jody K
JK
Takemoto
Rebecca M
RM
Bertram
Neal M
NM
Davies
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000097:blood* / Q000493:pharmacokinetics*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D006207:Half-Life; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D013267:Stilbenes / Q000097:blood* / Q000493:pharmacokinetics*
C476734:2,3',5',6-tetrahydroxy-trans-stilbene; D000894:Anti-Inflammatory Agents, Non-Steroidal; D013267:Stilbenes