Lipid component quantitation by thin layer chromatography.
This chapter describes a thin layer chromatography (TLC) method for the quantitation of various lipids (such as phospholipids, sphingolipids, acylglycerols, and fatty acids) in lipid-based nanoparticle formulations such as liposomes and nanoemulsions. We illustrate this technique to quantify C6-ceramide (N-hexanoyl-D: -erythro-sphingosine) in a nanoemulsion formulation. C6-ceramide is a powerful chemotherapeutic that is poorly soluble in aqueous buffers.
Nanotechnology Characterization Laboratory, Advanced Technology Program, SAIC-Frederick, Inc., National Cancer Institute at Frederick, Frederick, MD, USA.
Jeffrey D
JD
Clogston
Anil K
AK
Patri
D000818:Animals; D002518:Ceramides / Q000032:analysis*; D002855:Chromatography, Thin Layer / Q000379:methods*; D004655:Emulsions; D005227:Fatty Acids / Q000032:analysis; D005612:Freeze Drying; D006801:Humans; D008055:Lipids / Q000737:chemistry* / Q000302:isolation & purification; D008081:Liposomes; D053758:Nanoparticles / Q000737:chemistry; D010316:Particle Size; D010743:Phospholipids / Q000032:analysis; D012015:Reference Standards; D012995:Solubility; D013107:Sphingolipids / Q000032:analysis; D013110:Sphingosine / Q000031:analogs & derivatives* / Q000032:analysis; D013237:Stereoisomerism; D014867:Water / Q000737:chemistry
D002518:Ceramides; D004655:Emulsions; D005227:Fatty Acids; D008055:Lipids; D008081:Liposomes; D010743:Phospholipids; D013107:Sphingolipids; C481931:erythro-(2R,3S)-sphingosine; C101954:N-caproylsphingosine; D014867:Water; D013110:Sphingosine