Taxol content comparison in different parts of Taxus madia and Taxus chinensis var. mairei by HPLC.
OBJECTIVE
To analysis and compare the taxol content in different parts of Taxus madia and Taxus chinensis var. mairei by High Performance Liquid Chromatography (HPLC).


METHODS
85% EtOH and CH2Cl2 were used for the extraction of taxol. By HPLC, the methodology study and taxol content investigation were performed.


RESULTS
The taxol was extracted successfully. One simple and reliable methodology was built up. Basing on these, the taxol content in these two Taxus spp. were analysed and compared, among of which the leaf of Taxus chinensis var. mairei has the highest taxol content (5.18 x 10(-5), w/w).


CONCLUSIONS
Taxol and its content in the original plants can be simply and reliably extracted and investigated by these methods, which also can provided the scientific basis for the rational development of Taxus spp.
College of Pharmacy, Jinan University, Guangzhou 510632, China.
Quan-Feng
QF
Dong
Jing-Jing
JJ
Liu
Rong-Min
RM
Yu
D000972:Antineoplastic Agents, Phytogenic / Q000032:analysis* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003247:Conservation of Natural Resources; D000431:Ethanol / Q000737:chemistry; D017239:Paclitaxel / Q000032:analysis* / Q000302:isolation & purification; D024301:Plant Bark / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry*; D015203:Reproducibility of Results; D020946:Taxus / Q000737:chemistry* / Q000145:classification / Q000254:growth & development; D013678:Technology, Pharmaceutical / Q000379:methods; D014197:Trees / Q000737:chemistry / Q000254:growth & development
D000972:Antineoplastic Agents, Phytogenic; D000431:Ethanol; D017239:Paclitaxel