LC/ESI-MS n and 1H HR-MAS NMR analytical methods as useful taxonomical tools within the genus Cystoseira C. Agardh (Fucales; Phaeophyceae).
Species of the genus Cystoseira are particularly hard to discriminate, due to the complexity of their morphology, which can be influenced by their phenological state and ecological parameters. Our study emphasized on the relevance of two kinds of analytical tools, (1) LC/ESI-MS(n) and (2) (1)H HR-MAS NMR, also called in vivo NMR, to identify Cystoseira specimens at the specific level and discuss their taxonomy. For these analyses, samples were collected at several locations in Brittany (France), where Cystoseira baccata, C. foeniculacea, C. humilis, C. nodicaulis and C. tamariscifolia were previously reported. To validate our chemical procedure, the sequence of the ITS2 has been obtained for each species to investigate their phylogenetic relationships at a molecular level. Our study highlighted the consistency of the two physico-chemical methods, compared to "classical" molecular approach, in studying taxonomy within the genus Cystoseira. Especially, LC/ESI-MS(n) and phylogenetic analyses converged into the discrimination of two taxonomical groups among the 5 species. The occurrence of some specific signals in the (1)H HR-MAS NMR spectra and/or some characteristic chemical compounds during LC/ESI-MS(n) analysis could be regarded as discriminating factors. LC/ESI-MS(n) and (1)H HR-MAS NMR turned out to be two relevant and innovative techniques to discriminate taxonomically this complex genus.
Laboratoire d'Écophysiologie et de Biotechnologies des Halophytes et des Algues Marines EA 3877, Institut Universitaire Européen de la Mer, Université de Bretagne Occidentale, rue Dumont D'Urville, 29280 Plouzané, France. camille.jegou@univ-brest.fr
Camille
C
Jégou
Gérald
G
Culioli
Nelly
N
Kervarec
Gaëlle
G
Simon
Valérie
V
Stiger-Pouvreau
D002853:Chromatography, Liquid / Q000379:methods*; D004247:DNA / Q000235:genetics; D005602:France; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods; D000459:Phaeophyceae / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D010802:Phylogeny; D018521:Plant Physiological Phenomena; D016133:Polymerase Chain Reaction / Q000379:methods; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D004247:DNA