Interactive effect of fluoride burden with calcitonin receptor gene polymorphisms on the risk of F bone injury.
OBJECTIVE
This study aims to examine the interactive effect of fluoride burden with calcitonin receptor (CTR) gene polymorphisms on the risk of fluoride (F) bone injury and provide the basis for determination of F bone injury risk factors.


METHODS
In this case-control study, a total of 119 cases and 126 controls were enrolled from 2 aluminum plants in Hubei province. F burden (UF) was measured by F ion-selective electrode method. The CTR gene polymorphisms were determined using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Logistic regression analysis was used to estimate multivariate-adjusted odds ratios, 95% confidence intervals (CI).


RESULTS
The odds of developing F bone injury for participants in the moderate F burden group versus the mild F burden group were 4.1 (95% CI: 1.9, 8.7); the heavy F burden group versus the mild F burden group were 14.1 (95% CI: 6.5, 30.6). The odds of developing F bone injury for participants with the TC & TT genotypes versus the CC genotype were 2.6 (95% CI: 1.4, 4.7). The interactions between TC & TT genotypes and moderate, heavy F burden were significant (OR = 14.4; OR = 40.3).


CONCLUSIONS
The interactive effect of F burden and CTR genotype was significant, which increased the F bone injury risk.
Department of Occupational and Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Hubei, Wuhan 430030, People's Republic of China.
Jun
J
Tu
Kejian
K
Liu
Yu'e
Y
Song
Yuzeng
Y
Zhang
Caiyan
C
Cui
Cuirong
C
Lu
D000328:Adult; D001822:Body Burden; D001847:Bone Diseases / Q000175:diagnosis / Q000453:epidemiology / Q000209:etiology*; D001842:Bone and Bones / Q000187:drug effects; D016022:Case-Control Studies; D005459:Fluorides / Q000009:adverse effects / Q000097:blood*; D005838:Genotype; D006801:Humans; D008297:Male; D009784:Occupational Diseases / Q000175:diagnosis / Q000453:epidemiology / Q000209:etiology*; D016017:Odds Ratio; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D018003:Receptors, Calcitonin / Q000187:drug effects / Q000235:genetics*
D018003:Receptors, Calcitonin; D005459:Fluorides