SRY interacts with ribosomal proteins S7 and L13a in nuclear speckles.
The SRY (sex-determining region on the Y chromosome) is essential for male development; however, the molecular mechanism by which the SRY induces testis development is still unclear. To elucidate the mechanism of testis development, we identified SRY-interacting proteins using a yeast two-hybrid system. We found two ribosomal proteins, RPS7 (ribosomal protein S7) and RPL13a (ribosomal protein L13a) that interact with the HMG (high-mobility group) box domain of SRY. Furthermore, we confirmed the intracellular distributions of RPS7, RPL13a and SRY and found that the three proteins were co-expressed in COS1 cells. SRY, RPS7 and RPL13a were co-localized in nuclear speckles. These findings suggest that SRY plays an important role in activities associated with nuclear speckles via an unknown mechanism.
Department of Human Genetics and Public Health, Institute of Health Biosciences, Graduate School of Medicine, The University of Tokushima, Tokushima, Japan. sato@basic.med.tokushima-u.ac.jp
Youichi
Y
Sato
Shojiro
S
Yano
Ashraf A
AA
Ewis
Yutaka
Y
Nakahori
D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D006801:Humans; D008297:Male; D012269:Ribosomal Proteins / Q000032:analysis* / Q000378:metabolism*; D051876:Sex-Determining Region Y Protein / Q000032:analysis* / Q000378:metabolism*; D013737:Testis / Q000254:growth & development; D020798:Two-Hybrid System Techniques; D014998:Y Chromosome
C402487:RPL13A protein, human; D012269:Ribosomal Proteins; D051876:Sex-Determining Region Y Protein; C037442:ribosomal protein S7