Biocompatibility of tissue engineering constructions from porous polylactide carriers obtained by the method of selective laser sintering and bone marrow-derived multipotent stromal cells.
We studied the biocompatibility of porous polylactide carrier matrices obtained by means of surface selective laser sintering. Carrier matrices had no cytotoxic activity, but maintained adhesion and proliferation of cells. Subcutaneous transplantation of tissue engineering constructions from these carriers and bone marrow-derived multipotent stromal cells did not cause the inflammatory response and pathological changes in rats. The conditions for organotypic regeneration were provided at the site of transplantation (high degree of blood supply and considerable amount of immature precursor cells).
ReMeTeks Closed Corporation, Moscow, Russia. bukharovarmt@yandex.ru
T B
TB
Bukharova
E N
EN
Antonov
V K
VK
Popov
T Kh
TKh
Fatkhudinov
A V
AV
Popova
A V
AV
Volkov
S A
SA
Bochkova
V N
VN
Bagratashvili
D V
DV
Gol'dshtein
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D002448:Cell Adhesion / Q000502:physiology*; D005434:Flow Cytometry; D048091:Guided Tissue Regeneration / Q000379:methods*; D006358:Hot Temperature; D006801:Humans; D016130:Immunophenotyping; D007834:Lasers; D008297:Male; D008422:Materials Testing / Q000379:methods; D008855:Microscopy, Electron, Scanning; D039902:Multipotent Stem Cells / Q000166:cytology*; D011091:Polyesters / Q000737:chemistry*; D016062:Porosity; D051381:Rats; D033581:Stem Cell Transplantation / Q000379:methods; D017154:Stromal Cells / Q000166:cytology; D013778:Tetrazolium Salts; D013844:Thiazoles; D023822:Tissue Engineering / Q000379:methods*
D011091:Polyesters; D013778:Tetrazolium Salts; D013844:Thiazoles; C033616:poly(lactide); C022616:thiazolyl blue