[Transmembrane transport and metabolism of diammonium glycyrrhizinate across rat small intestine in Ussing Chamber].
OBJECTIVE
To explore the transmembrane transport and metabolism of diammonium glycyrrhizinate in intestines of rats.


METHODS
An Ussing Chamber model were used to investigate the transmembrane transport of diammonium glycyrrhizinate (GZ), the concentrations of diammonium glycyrrhizinate and its two metabolites were determined by HPLC.


RESULTS
The permeability coefficients of GZ in difference intestinal mucous membranes were ranged from 0.3 x 10(-6) cm x s(-1) to 1.1 x 10(-6) cm x s(-1). The metabolism of GZ in enterocytes during its transport process was negligible. The concentration of diammonium glycyrrhizinate and pH had limit effects on the transport amount and the permeability coefficients of GZ.


CONCLUSIONS
GZ is a low permeability drug, but it can be absorbed at all segments of the small intestine in rats. Ileum is the major absorption region of GZ.
Key Lab of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, Nanjing 210009, China.
Guofu
G
Li
Jin
J
Yang
Xiaoyi
X
Hua
Rui
R
Chen
Haoru
H
Zhao
D000818:Animals; D001692:Biological Transport; D002463:Cell Membrane Permeability; D019695:Glycyrrhizic Acid / Q000378:metabolism / Q000493:pharmacokinetics*; D006863:Hydrogen-Ion Concentration; D007421:Intestine, Small / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008954:Models, Biological; D051381:Rats; D017207:Rats, Sprague-Dawley
D019695:Glycyrrhizic Acid