Evaluation of the resistance of DNA immobilized on ferrimagnetic particles of cobalt ferrite nanopowder against nuclease cleavage.
DNA was immobilized on ferrimagnetic particles of cobalt ferrite nanopowder (CoFe(2)O(4)) and its resistance to endonuclease (DNase I) hydrolysis was studied. Immobilization on cobalt ferrite nanoparticles prevented enzymatic cleavage of DNA. This process was not associated with enzyme inactivation under the effect of nanosize cobalt ferrite and was presumably determined by lesser availability of the DNA molecule as a result of its interaction with nanoparticles.
Siberian State Medical University, Tomsk, Russia. allysyz@mail.ru
A G
AG
Pershina
A E
AE
Sazonov
L M
LM
Ogorodova
D003035:Cobalt / Q000737:chemistry*; D004247:DNA / Q000737:chemistry* / Q000378:metabolism*; D003850:Deoxyribonuclease I / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D013053:Spectrophotometry
D058185:Magnetite Nanoparticles; D003035:Cobalt; D004247:DNA; D003850:Deoxyribonuclease I