[Effects of clotrimazole on the growth of oral squamous cell carcinoma].
OBJECTIVE
to investigate the effects of clotrimazole on the growth of oral squamous cell carcinoma (OSCC).


METHODS
OSCC-25 cells growing in log phase were treated with various doses of clotrimazole. The concentration of IC(50), cell cycle and cell cycle related protein were examined.


RESULTS
the concentration of clotrimazole for inhibiting OSCC was IC(50) 8.51 µmol/L. Clotrimazole induced cell cycle arrest in the G(0)-G(1) cell cycle phase, with a concomitant decrease of cells in the G(2)-M and S-phase. Furthermore, clotrimazole significantly decreased the levels of cyclin D, cyclin E and CDK-4.


CONCLUSIONS
clotrimazole inhibits the growth of OSCC.
Department of Oral and Maxillofacial Surgery, Xiangya Hospital School of Stomatology, Central South University, Changsha 410078, China.
Hui
H
Liu
Yue-Hong
YH
Wang
Zhan-Gui
ZG
Tang
Ou-Sheng
OS
Liu
Jie
J
Zhang
Gui-Yuan
GY
Li
D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D002453:Cell Cycle; D059447:Cell Cycle Checkpoints; D002455:Cell Division; D045744:Cell Line, Tumor; D003022:Clotrimazole / Q000494:pharmacology*; D019927:Cyclin E; D006801:Humans; D009062:Mouth Neoplasms / Q000473:pathology*; D015513:Oncogene Proteins
C475820:CCNE1 protein, human; D019927:Cyclin E; D015513:Oncogene Proteins; D003022:Clotrimazole