Relationship between suppression of E6 and E7 virus oncogenes and expression of apoptosis and cell cycle genes in cervical cancer culture.
The effects of short interfering RNA suppressing the expression of E6 and E7 human papilloma virus (type 18) on the expression of apoptosis and cell cycle genes were studied in HeLa cells. Changes in the transcription profiles were evaluated using DNA microarray and real-time reverse-transcription PCR. Cell transfection with anti-E6 and anti-E7 short interfering RNA moderately reduced the expression of mRNA for CDC25C, GRB2, GTSE1, and PLK1 genes and induced expression of CDKN1A (p21(CIP)) gene mRNA. In addition, culture proliferation was inhibited and morphological changes characteristic of differentiation and cell aging developed.
Russian State Medical University, Moscow, Russia.
E V
EV
Khokhlova
A N
AN
Shkoporov
N N
NN
Volodin
B A
BA
Efimov
K A
KA
Pavlov
L I
LI
Kafarskaia
D017209:Apoptosis / Q000187:drug effects* / Q000235:genetics; D018797:Cell Cycle Proteins / Q000378:metabolism; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics; D004268:DNA-Binding Proteins / Q000378:metabolism*; D051380:GRB2 Adaptor Protein / Q000378:metabolism; D020869:Gene Expression Profiling; D015967:Gene Expression Regulation, Viral / Q000187:drug effects*; D018816:Genes, cdc / Q000187:drug effects*; D006367:HeLa Cells; D006801:Humans; D046228:Microarray Analysis; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D009856:Oncogene Proteins, Viral / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D034741:RNA, Small Interfering / Q000494:pharmacology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020687:cdc25 Phosphatases / Q000378:metabolism; D000097869:Polo-Like Kinase 1
D018797:Cell Cycle Proteins; D050759:Cyclin-Dependent Kinase Inhibitor p21; D017931:DNA Primers; D004268:DNA-Binding Proteins; D051380:GRB2 Adaptor Protein; D008869:Microtubule-Associated Proteins; D009856:Oncogene Proteins, Viral; D017346:Protein Serine-Threonine Kinases; D011518:Proto-Oncogene Proteins; D034741:RNA, Small Interfering; D020687:cdc25 Phosphatases; D000097869:Polo-Like Kinase 1; C052603:E6 protein, Human papillomavirus type 18; C052633:E7 protein, Human papillomavirus type 18; C495948:GRB2 protein, human; C115080:GTSE1 protein, human; C517009:CDC25C protein, human