Production of lantipeptides in Escherichia coli.
Lantipeptides are ribosomally synthesized and posttranslationally modified peptides containing thioether cross-links. We describe the preparation of seven different lantipeptides in Escherichia coli and demonstrate that this methodology can be used to incorporate nonproteinogenic amino acids.
Howard Hughes Medical Institute, Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA.
Yanxiang
Y
Shi
Xiao
X
Yang
Neha
N
Garg
Wilfred A
WA
van der Donk
D000596:Amino Acids / Q000737:chemistry; D004926:Escherichia coli / Q000378:metabolism*; D010455:Peptides / Q000737:chemistry / Q000378:metabolism*; D011487:Protein Conformation; D011499:Protein Processing, Post-Translational; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013438:Sulfhydryl Compounds / Q000737:chemistry / Q000378:metabolism
D000596:Amino Acids; D010455:Peptides; D013438:Sulfhydryl Compounds