Subacute proteome changes following traumatic injury of the developing brain: Implications for a dysregulation of neuronal migration and neurite arborization.
Traumatic brain injury (TBI) is a major cause of morbidity and mortality among children and adolescents. To gain insight into developmental events influenced by TBI, we analyzed subacute mouse brain proteome changes in a percussion head trauma model at P7 ipsi- and contralateral to the site of injury. The comparison of brain proteomes of trauma mice and controls revealed reproducible changes in the intensity of 28 proteins (30 protein spots) in response to trauma. The changes detected suggest that TBI leads to apoptosis, inflammation, and oxidative stress. These changes were consistent with our results of histological and biochemical evaluation of the brains which revealed widespread apoptotic neurodegeneration, microglia activation, and increased levels of protein carbonyls. Furthermore, we detected changes in proteins involved in neuronal migration as well as axonal and dendritic growth and guidance, suggesting interference of trauma with these developmental events.
Department of Pediatric Neurology, Charité, University Medicine Berlin, Campus Virchow-Klinikum, Berlin, Germany; Institute of Human Genetics, Charité, University Medicine Berlin, Campus Virchow-Klinikum, Berlin, Germany; Department of Pediatric Neurology, University Childrens' Hospital, Technical University Dresden, Dresden, Germany. angela.kaindl@charite.de.
Angela M
AM
Kaindl
Claus
C
Zabel
Vanya
V
Stefovska
Ron
R
Lehnert
Marco
M
Sifringer
Joachim
J
Klose
Chrysanthy
C
Ikonomidou