Simultaneous extraction and analysis by high performance liquid chromatography coupled to diode array and mass spectrometric detectors of bixin and phenolic compounds from annatto seeds.
This study was designed to identify and quantify the carotenoids and phenolic compounds from annatto seeds using high performance liquid chromatography coupled to diode array and mass spectrometer detectors (HPLC-DAD-MS/MS). Furthermore, using response surface methodology, an optimized procedure for simultaneous extraction of these compounds was established. In addition to bixin, known to be the main carotenoid in annatto seeds, hypolaetin and a caffeoyl acid derivative were identified as the main phenolic compounds. The optimized procedure involved 15 extractions using acetone:methanol:water (50:40:10, v/v/v) as solvent, a solid-liquid ratio of 1:9 (m/v) and an extraction time of 5 min. Validation data indicated that the HPLC method proposed provided good linearity, sensitivity, procedure accuracy, system precision and suggested its suitability for the simultaneous analysis of phenolic compounds and carotenoids in annatto seeds.
Department of Food Science, Faculty of Food Engineering, University of Campinas (UNICAMP), PO Box 6121, Rua Monteiro Lobato, 80, CEP: 13083-862, Campinas, São Paulo, Brazil.
Renan Campos
RC
Chisté
Fábio
F
Yamashita
Fábio Cesar
FC
Gozzo
Adriana Zerlotti
AZ
Mercadante
D031212:Bixaceae / Q000737:chemistry*; D002338:Carotenoids / Q000032:analysis* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D016014:Linear Models; D008956:Models, Chemical; D010636:Phenols / Q000032:analysis* / Q000302:isolation & purification; D015203:Reproducibility of Results; D012639:Seeds / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000379:methods*
D010636:Phenols; D002338:Carotenoids; C004588:bixin