Method development for β-glucogallin and gallic acid analysis: application to urinary pharmacokinetic studies.
A reversed phase high-pressure liquid chromatographic electrospray tandem mass spectrometry method was developed for the simultaneous detection and analysis of β-glucogallin and gallic acid. This method used a C(18) column with ultraviolet detection at 285 nm, ionization in the negative ion mode for β-glucogallin and gallic acid, and in the positive ion mode for the internal standard 3-morpholin-4-ylpropane-1-sulfonic acid. Mobile phase consisted of a mixture of water, methanol, and formic acid at a flow rate of 0.7 mL/min. This method was validated over a concentration range of 1-100 μg/mL in rat urine.
College of Pharmacy, Department of Pharmaceutical Sciences and Graduate Program in Pharmaceutical Sciences, Washington State University, Pullman, WA 99164-6534, USA.
Jody K
JK
Takemoto
Neal M
NM
Davies
D000818:Animals; D001688:Biological Products / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D005240:Feasibility Studies; D005707:Gallic Acid / Q000737:chemistry / Q000493:pharmacokinetics* / Q000652:urine*; D047348:Hydrolyzable Tannins / Q000737:chemistry / Q000493:pharmacokinetics* / Q000652:urine*; D057230:Limit of Detection; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
D001688:Biological Products; D047348:Hydrolyzable Tannins; C060474:glucogallin; D005707:Gallic Acid