[Experimental study on anti-inflammatory effect of adenovirus-mediated sTLR2 in LPS-induced murine inflammation].
OBJECTIVE
To observe the anti-inflammatory effect of adenovirus-mediated sTLR2 in LPS-induced murine inflammation, and to provide a new thinking and tactics of prevention and treatment for acute inflammatory reaction.


METHODS
Murine inflammation model induced by LPS (lethal dose) was established. BALB/c mice were divided into normal control group, inflammation model group and AdTLR2 treatment group. The recombinant adenovirus (Ad)TLR2 acted on test mice respectively before and after inflammation production, the impact of AdTLR2 on murine survival time was observed. Serum samples of different experiment groups were collected, alteration of cytokine TNF-alpha, IL-1beta, IL-6, IL-10 and IL-13 were tested by ELISA, the influence of AdTLR2 on serum level of cytokines were evaluated.


RESULTS
A prolonged survival time by AdTLR2 was observed on lethal dose of LPS animal model; serum level of TNF-alpha, IL-1beta, IL-6, IL-10 and IL-13 were reduced by AdTLR2 on LPS induced murine model, indicating a certain immuno-regulation and anti-inflammatory effect of recombinant AdTLR2.


CONCLUSIONS
Recombinant AdTLR2 resulted a prolong survival time of LPS induced inflammation animal model and reduction of serum level of TNF-alpha, IL-1beta, IL-6, IL-10 and IL-13, which achieved a certain immuno-regulation and anti-inflammatory effect.
Department of Clinical Laboratory, No. 201 Hospital of PLA, Liaoyang 111000, China. sunshouxun_79@163.com
Shou-Xun
SX
Sun
Qiang
Q
Li
Yan
Y
Shi
Jing
J
Liu
Xiao-Yun
XY
Pu
D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D000893:Anti-Inflammatory Agents; D016207:Cytokines / Q000097:blood; D005260:Female; D007249:Inflammation / Q000097:blood / Q000139:chemically induced* / Q000235:genetics / Q000628:therapy*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D051195:Toll-Like Receptor 2 / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*
D000893:Anti-Inflammatory Agents; D016207:Cytokines; D008070:Lipopolysaccharides; D011994:Recombinant Proteins; C495342:TLR2 protein, human; D051195:Toll-Like Receptor 2