Androgen- and insulin-related gene signature using a specific low density oligoarray AndroChip 2 in peripheral blood mononuclear cells in agonists, recreational athletes and sedentary subjects.
The early detection of genomic biomarkers (e.g. RNAs) through analysis of circulating blood cells could have a substantial impact on biomedicine, particularly in monitoring clinical trials, drug toxicity and doping in athletes. To achieve this goal, it is essential to develop methods that are sufficiently sensitive to detect biomarker alterations during normal biologic processes, pathogenic processes, and or in response to therapeutic or other intervention. Using a low density microarray (AndroChip 2) we detected a transcriptional profiling signature of 190 genes related to androgen and insulin metabolism pathway, in peripheral blood mononuclear cell (PBMC) in subjects with different intensities of sports activities. We demonstrated that androgen and insulin gene transcriptional levels are independent to sports activity and therefore potentially suitable for drug monitoring and/or drug doping (such as anabolic androgen steroid AAS abuse) and or gene doping.
Department of Biopathology, Tor Vergata University, Fatebenefratelli Hospital S. Pietro, Rome, Italy.
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D
Minella
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Biancolella
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Testa
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Prosperini
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Zenobi
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Novelli
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Giganti
D000293:Adolescent; D000328:Adult; D000728:Androgens / Q000235:genetics* / Q000378:metabolism*; D056352:Athletes; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D015444:Exercise / Q000502:physiology*; D020869:Gene Expression Profiling; D005819:Genetic Markers; D006801:Humans; D007328:Insulin / Q000235:genetics* / Q000378:metabolism*; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055815:Young Adult
D000728:Androgens; D017931:DNA Primers; D005819:Genetic Markers; D007328:Insulin