Hypoxia-mediated down-regulation of OCTN2 and PPARα expression in human placentas and in BeWo cells.
The objective of the present study was to investigate the effects of hypoxia on placental expression of OCTN2 and PPARα. OCTN2 and PPARα expression in the human placenta in the presence or absence of preeclampsia was examined by immunohistochemical (IHC) analysis, Western blotting, and quantitative polymerase chain reaction (qPCR). Effects of hypoxia on the expression of OCTN2 and PPARα in human placental explants and human choriocarcinoma BeWo cells were examined by Western blotting and qPCR analyses. IHC, Western blot, and qPCR studies showed that OCTN2 and PPARα protein and mRNA levels were lower in syncytiotrophoblasts from preeclamptic human placentas than in those from normal placentas. Hypoxic treatment caused a decrease in OCTN2 and PPARα expression in human placental explants and in BeWo cells. WY14643, a PPARα agonist, caused an increase in OCTN2 expression in BeWo cells under hypoxic conditions. In conclusion, under hypoxic conditions, placental OCTN2 is down-regulated through PPARα-mediated pathways.
School of Pharmacy, College of Medicine, National Taiwan University, Taipei 100, Taiwan.
Ting-Ting
TT
Chang
Ming-Kwang
MK
Shyu
Min-Chuan
MC
Huang
Chen-Chi
CC
Hsu
Szu-Yu
SY
Yeh
Mei-Ru
MR
Chen
Chun-Jung
CJ
Lin
D000328:Adult; D051792:Basic Helix-Loop-Helix Proteins / Q000378:metabolism; D015153:Blotting, Western; D002331:Carnitine / Q000378:metabolism; D015687:Cell Hypoxia / Q000502:physiology*; D002478:Cells, Cultured; D005576:Colforsin / Q000494:pharmacology; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D027701:Organic Cation Transport Proteins / Q000235:genetics / Q000378:metabolism*; D047493:PPAR alpha / Q000235:genetics / Q000378:metabolism*; D010920:Placenta / Q000187:drug effects / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D000074058:Solute Carrier Family 22 Member 5; D046509:Tissue Culture Techniques; D000099162:Endothelial PAS Domain-Containing Protein 1
D051792:Basic Helix-Loop-Helix Proteins; D002331:Carnitine; D005576:Colforsin; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D027701:Organic Cation Transport Proteins; D047493:PPAR alpha; D000074058:Solute Carrier Family 22 Member 5; D000099162:Endothelial PAS Domain-Containing Protein 1; C497442:HIF1A protein, human; C492020:SLC22A5 protein, human