An IgG-binding protein A homolog in Staphylococcus hyicus.
Shotgun phage display was used to identify a homolog of the IgG-binding protein staphylococcal protein A in Staphylococcus hyicus type strain CCUG 15602/ATCC 11249. This bacterium is the causative agent of exudative epidermitis in pigs and can also cause mastitis in cattle. A protein with similar features as the originally identified protein A in Staphylococcus aureus was described; an YSIRK-type signal peptide, four IgG-binding domains, a putative peptidoglycan-binding domain, and a cell wall anchoring motif (LPXTG) was present. The highest degree of similarity was to a protein A homolog in Staphylococcus pseudintermedius. However, typical Xr polypeptide repeats present in the protein A of S. aureus and S. pseudintermedius could not be identified in the protein A of S. hyicus. The presence of the spa gene in ten porcine and eight bovine clinical isolates of S. hyicus was investigated by PCR. In all isolates, the spa gene could be detected but the amplicons were of two sizes. Sequence analysis of four selected PCR amplicons showed that only three IgG-binding domains were present in the protein A of clinical isolates generating a smaller spa fragment. The finding of spa in S. hyicus contributes to an increased understanding of potential virulence factors in this species.
Department of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences, Box 7009, SE-750 07 Uppsala, Sweden. anna.rosander@bvf.slu.se
Anna
A
Rosander
Bengt
B
Guss
Märit
M
Pringle
D020816:Amino Acid Motifs; D000818:Animals; D001665:Binding Sites; D004269:DNA, Bacterial / Q000235:genetics; D019151:Peptide Library; D016133:Polymerase Chain Reaction / Q000662:veterinary; D021382:Protein Sorting Signals / Q000235:genetics; D017422:Sequence Analysis, DNA; D013203:Staphylococcal Infections / Q000382:microbiology / Q000662:veterinary; D013205:Staphylococcal Protein A / Q000235:genetics*; D013211:Staphylococcus aureus / Q000235:genetics; D057788:Staphylococcus hyicus / Q000235:genetics*; D013552:Swine / Q000382:microbiology; D013553:Swine Diseases / Q000382:microbiology
D004269:DNA, Bacterial; D019151:Peptide Library; D021382:Protein Sorting Signals; D013205:Staphylococcal Protein A