The functional class evaluated in rheumatoid arthritis is associated with soluble TGF-β1 serum levels but not with G915C (Arg25Pro) TGF-β1 polymorphism.
The influence of genetic factors in rheumatoid arthritis (RA) has been described, including several cytokine genes such as transforming growth factor β (TGF-β) with regulatory effects on lymphocytes, dendritic cells, macrophages, chondrocytes, and osteoblasts, which are important in the RA pathogenesis. The G915C TGF-β1 polymorphism has been associated with soluble TGF-β1 (sTGF-β) serum levels. Thus, we studied the association of G915C (Arg25Pro) TGF-β1 polymorphism with sTGF-β1 serum levels in RA. We enrolled 120 RA patients and 120 control subjects (CS). The G915C TGF-β1 polymorphism was determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method, and sTGF-β1 serum levels were quantified using an ELISA kit. The genotype frequency of G915C TGF-β1 polymorphism in RA and CS was G/G (91.7%), G/C (8.3%), C/C (0%) and G/G (85.8%), G/C (14.2%), C/C (0%), respectively, without significant differences. Moreover, the G/G TGF-β1 genotype carriers presented the highest disability index evaluated for the Spanish HAQ-DI score (P < 0.001). In addition, the sTGF-β1 serum levels were higher in RA (182.2 ng/mL) than CS (160.2 ng/mL), there was not significant difference. However, we found a positive correlation between the sTGF-β1 serum levels and the functional class (r = 0.472, P = 0.023). In conclusion, the G915C (Arg25Pro) TGF-β1 polymorphism is not associated with RA, but the sTGF-β1 serum levels are related with the functional class in RA.
Departamento de Biología Molecular y Genómica, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Insurgentes 244-1, Colonia Lomas de Atemajac, C.P. 45178 Guadalajara, Zapopan, Jalisco, Mexico. biologiamolecular@hotmail.com
José Francisco
JF
Muñoz-Valle
Nora Magdalena
NM
Torres-Carrillo
Iris Paola
IP
Guzmán-Guzmán
Norma
N
Torres-Carrillo
Sandra Luz
SL
Ruiz-Quezada
Claudia Azucena
CA
Palafox-Sánchez
Héctor
H
Rangel-Villalobos
María Guadalupe
MG
Ramírez-Dueñas
Isela
I
Parra-Rojas
Mary
M
Fafutis-Morris
Blanca Estela
BE
Bastidas-Ramírez
Ana Laura
AL
Pereira-Suárez
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D019943:Amino Acid Substitution / Q000235:genetics*; D001172:Arthritis, Rheumatoid / Q000145:classification* / Q000208:ethnology / Q000235:genetics*; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008800:Mexico / Q000208:ethnology; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D053773:Transforming Growth Factor beta1 / Q000097:blood* / Q000145:classification / Q000235:genetics*; D055815:Young Adult
D053773:Transforming Growth Factor beta1