Quantitation of nanoparticles in serum matrix by capillary electrophoresis.
Sensitive and fast analytical techniques are needed to determine the concentration of nanoparticles in biological samples (e.g., blood and tissues) for biodistribution and toxicity studies. This chapter describes a method for the use of capillary zone electrophoresis (CZE) and micellar electrokinetic chromatography (MEKC) for the quantitation of fullerene nanoparticles in human serum matrix. Data on the fullerene-based nanoparticle carboxyfullerene (C3 fullerene) in human serum is presented as an example.
Laboratory of Proteomics and Analytical Technologies, Advanced Technology Program, SAIC-Frederick, Inc., National Cancer Institute at Frederick, Frederick, MD, USA.
King C
KC
Chan
Timothy D
TD
Veenstra
Haleem J
HJ
Issaq
D002138:Calibration; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000379:methods*; D037741:Fullerenes / Q000032:analysis / Q000097:blood / Q000493:pharmacokinetics; D006801:Humans; D053758:Nanoparticles / Q000032:analysis*; D012015:Reference Standards; D044967:Serum / Q000737:chemistry*; D014018:Tissue Distribution
D037741:Fullerenes