Electrochemiluminescent biosensing of carbohydrate-functionalized CdS nanocomposites for in situ label-free analysis of cell surface carbohydrate.
A facile electrochemiluminescent (ECL) strategy for in situ label-free monitoring of carbohydrate expression on living cells was designed by integrating the specific recognition of lectin to carbohydrate with a carbohydrate-functionalized CdS nanocomposite. The mercaptopropionic acid-capped CdS quantum dots were firstly immobilized on carbon nanotubes modified electrode and then functionalized with carbohydrate using mannan as a model on the surface. The carbohydrate-functionalized CdS nanocomposite showed high ECL sensitivity and good stability, and could be used for competitive recognition to concanavalin A with the target cells in solution, which led to a change of ECL intensity due to the resistance of concanavalin A. The change depended on both the cell number and the expression level of cell surface carbohydrate. A wide linear response to cells ranging from 2×10(3) to 1×10(7) cells mL(-1) with a detection limit of 1.2×10(3) cells mL(-1) was obtained. The proposed biosensor could be used to in situ evaluate cell surface glycan, and the average number of mannose moieties on single living BGC cell was detected to be 8.7×10(7). This sensitive strategy was further used for facile monitoring of dynamic carbohydrate expression on living cells in response to drugs. The proposed method could be further expanded to high-throughput detection with the addition of more specific glycan-lectin pairs to the repertoire.
Key Laboratory of Analytical Chemistry for Life Science (Ministry of Education of China), Department of Chemistry, Nanjing University, Nanjing 210093, PR China.
En
E
Han
Lin
L
Ding
Shi
S
Jin
Huangxian
H
Ju
D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation*; D019187:Cadmium Compounds / Q000737:chemistry*; D002241:Carbohydrates / Q000032:analysis* / Q000737:chemistry*; D002462:Cell Membrane / Q000378:metabolism*; D003217:Conductometry / Q000295:instrumentation; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D008163:Luminescent Measurements / Q000295:instrumentation*; D045663:Quantum Dots; D013194:Staining and Labeling; D013440:Sulfides / Q000737:chemistry*
D019187:Cadmium Compounds; D002241:Carbohydrates; D013440:Sulfides; C034939:cadmium sulfide