[Establishment of loop-mediated isothermal amplification (LAMP) for detecting Pneumocystis carinii].
Animal model of Pneumocystis carinii pneumonia (PCP) was established for acquiring lung tissue infected with P. carinii. After DNA from rat lungs was extracted, nuclear ribosome small subunit 18s rDNA of P. carinii was amplified by loop-mediated isothermal amplification method at 63 degrees C for 60 min. The product was digested by restriction enzyme Apal I. The results showed that 18s ribosome DNA (rDNA) of P. carinii was cloned into vector pGEX6p2, and the positive clones screened. Therefore, the loop-mediated isothermal amplification has been established for detecting P. carinii.
Institute of Pathogenic Biology and Immunology, Hebei North University, Zhangjiakou 075000, China.
Hui
H
Zhang
Xue-qing
XQ
Liu
Rong-zhi
RZ
He
Tian-jun
TJ
Jia
Jin-shun
JS
Zhang
D000818:Animals; D017931:DNA Primers / Q000235:genetics; D042622:DNA Repeat Expansion; D004271:DNA, Fungal; D004275:DNA, Ribosomal / Q000235:genetics; D004195:Disease Models, Animal; D005260:Female; D008168:Lung / Q000382:microbiology*; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D045363:Pneumocystis carinii / Q000235:genetics / Q000302:isolation & purification*; D011020:Pneumonia, Pneumocystis / Q000175:diagnosis*; D051381:Rats; D017208:Rats, Wistar
D017931:DNA Primers; D004271:DNA, Fungal; D004275:DNA, Ribosomal