Streptococcus suis infection induces [corrected] bacterial accumulation in the kidney.
Although a Streptococcus suis infection was recently expanded, the study of S. suis has not had enough attention. In this study, bacterial kinetics of S. suis were investigated for seven days of infection in a mouse model. Survival rates showed that 5.0 × 10(7) CFU ml(-1) of S. suis infection caused death in 30% of mice subjects. Streptococcal kinetics from 10(4) to 10(7) CFU ml(-1) were detected in the brain, kidney, lung, spleen and urine two days post-infection and still detected in both kidney and urine seven days post-infection. In addition, 5.0 × 10(6) CFU ml(-1) of low dose infection was conducted, showing that S. suis was detected in the kidney seven days post-infection. Expressions of virulence-associated genes (mrp, epf, sly and hyl) were evaluated and results showed that all virulence-associated genes were detected in both spleen and kidney, whereas no detectable bands in either lung and liver two days post-infection. Microscopy demonstrated that S. suis accumulated in the renal cortex side of blood vessels and renal tubules two days post-infection, while S. suis accumulated in the renal tubule seven days post-infection. This is the first report of accumulated S. suis in mice kidney during S. suis infection.
International Research Center for Infectious Diseases, Research Institute for Microbial Diseases, Osaka University, Japan. ta-tsu@biken.osaka-u.ac.jp
Tatsuya
T
Nakayama
Dan
D
Takeuchi
Yukihiro
Y
Akeda
Kazunori
K
Oishi
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D005260:Female; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D007668:Kidney / Q000382:microbiology*; D007674:Kidney Diseases / Q000382:microbiology* / Q000401:mortality; D051379:Mice; D011897:Random Allocation; D013047:Specific Pathogen-Free Organisms; D013290:Streptococcal Infections / Q000382:microbiology* / Q000401:mortality; D017011:Streptococcus suis / Q000235:genetics / Q000502:physiology*
D001426:Bacterial Proteins