Improved enzymatic production of phenolated acylglycerols through alkyl phenolate intermediates.
A novel approach is reported for the synthesis of dihydrocaffoylated glycerols that consists of two steps: enzymatic synthesis of octyl dihydrocaffeate (as a synthetic intermediate) from octanol and dihydrocaffeic acid, and enzymatic interesterification of triacylglycerols with octyl dihydrocaffeate. Due to the good compatibility of the intermediate with triacylglycerols, an improved volumetric productivity [147 mol h(-1)(kg Novozym 435)(-1)] and high enzyme specific activity [up to 9.6 μmol(-1) min(-1)(g Novozym 435)(-1)] have been obtained.
Department of Molecular Biology, University of Aarhus, Aarhus C, Denmark.
Zhiyong
Z
Yang
Vivian
V
Feddern
Marianne
M
Glasius
Zheng
Z
Guo
Xuebing
X
Xu
D001709:Biotechnology / Q000379:methods*; D002109:Caffeic Acids / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D005989:Glycerides / Q000138:chemical synthesis* / Q000737:chemistry; D008049:Lipase / Q000378:metabolism; D015394:Molecular Structure; D010636:Phenols / Q000737:chemistry*
D002109:Caffeic Acids; D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D005989:Glycerides; D010636:Phenols; C000995:3,4-dihydroxyphenylpropionic acid; C093667:Novozyme 435; D008049:Lipase