Ozonated sesame oil enhances cutaneous wound healing in SKH1 mice.
Ozone is well recognized as a bactericidal agent and its beneficial effect on wound healing could be a consequence of this property. Because ozone itself does not penetrate the cells but immediately reacts with polyunsaturated fatty acids, its effects should be the results of oxidative reaction. For this reason, ozonated oils could be a way to deliver ozone messengers to the skin. This paper evaluated the therapeutic effects of three different grades of ozonated sesame oil in acute cutaneous wounds made in the skin of SKH1 mice. Specifically, wound closure rate, histological parameters, and the level of key proteins such as vascular endothelial growth factors and cyclin D1 have been analyzed in relation to the peroxide level present in the ozonated oil. Treatment with moderately ozonated sesame oil--expressed as peroxide value about 1,500)--has a faster wound closure rate in the first 7 days than treatment with oil containing either lower or higher peroxide value, and even with controls. Moreover, under the same treatment, an earlier and higher response of cells involved in wound repair, a higher angiogenesis, as well as an enhanced vascular endothelial growth factors and cyclin D1 expression were observed. The present study shows the validity of ozonated sesame oil in cutaneous wound healing and emphasizes the importance of the ozonation grade.
Dipartimento di Scienze Biomediche, Università degli Studi di Siena, Siena, Italy.
Giuseppe
G
Valacchi
Yunsook
Y
Lim
Giuseppe
G
Belmonte
Clelia
C
Miracco
Iacopo
I
Zanardi
Velio
V
Bocci
Valter
V
Travagli
D000818:Animals; D019938:Cyclin D1 / Q000378:metabolism; D005260:Female; D051379:Mice; D008812:Mice, Hairless; D010083:Oxidants, Photochemical / Q000494:pharmacology / Q000627:therapeutic use*; D010126:Ozone / Q000494:pharmacology / Q000627:therapeutic use*; D012715:Sesame Oil / Q000494:pharmacology / Q000627:therapeutic use*; D012867:Skin / Q000187:drug effects / Q000293:injuries* / Q000378:metabolism; D013997:Time Factors; D042442:Vascular Endothelial Growth Factors / Q000378:metabolism; D014945:Wound Healing / Q000187:drug effects*; D014950:Wounds, Penetrating / Q000378:metabolism / Q000473:pathology / Q000628:therapy*
C530105:Ccnd1 protein, mouse; D010083:Oxidants, Photochemical; D042442:Vascular Endothelial Growth Factors; D019938:Cyclin D1; D010126:Ozone; D012715:Sesame Oil