Exogenous coenzyme Q10 modulates MMP-2 activity in MCF-7 cell line as a breast cancer cellular model.
OBJECTIVE
Matrix Metalloproteinases 2 is a key molecule in cellular invasion and metastasis. Mitochondrial ROS has been established as a mediator of MMP activity. Coenzyme Q(10) contributes to intracellular ROS regulation. Coenzyme Q(10) beneficial effects on cancer are still in controversy but there are indications of Coenzyme Q(10) complementing effect on tamoxifen receiving breast cancer patients.


METHODS
In this study we aimed to investigate the correlation of the effects of co-incubation of coenzyme Q10 and N-acetyl-L-cysteine (NAC) on intracellular H2O2 content and Matrix Metalloproteinase 2 (MMP-2) activity in MCF-7 cell line.


CONCLUSIONS
Our experiment was designed to assess the effect in a time and dose related manner. Gelatin zymography and Flowcytometric measurement of H2O2 by 2'7',-dichlorofluorescin-diacetate probe were employed. The results showed that both coenzyme Q10 and N-acetyl-L-cysteine reduce MMP-2 activity along with the pro-oxidant capacity of the MCF-7 cell in a dose proportionate manner.


CONCLUSIONS
Collectively, the present study highlights the significance of Coenzyme Q(10) effect on the cell invasion/metastasis effecter molecules.
Department of Clinical Biochemistry, Tehran University of Medical Sciences, Faculty of Medicine, Tehran, Iran.
Massih
M
Bahar
Shahnaz
S
Khaghani
Parvin
P
Pasalar
Maliheh
M
Paknejad
Mohammad Reza
MR
Khorramizadeh
Hossein
H
Mirmiranpour
Siavash Gerayesh
SG
Nejad
D000111:Acetylcysteine / Q000494:pharmacology; D015415:Biomarkers / Q000378:metabolism; D001943:Breast Neoplasms / Q000378:metabolism*; D019328:Buthionine Sulfoximine / Q000494:pharmacology; D002277:Carcinoma / Q000378:metabolism*; D045744:Cell Line, Tumor; D015536:Down-Regulation / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D016166:Free Radical Scavengers / Q000494:pharmacology; D005721:Glutamate-Cysteine Ligase / Q000037:antagonists & inhibitors; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D009994:Osmolar Concentration; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects; D013997:Time Factors; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000378:metabolism
D015415:Biomarkers; D004791:Enzyme Inhibitors; D016166:Free Radical Scavengers; D014451:Ubiquinone; D019328:Buthionine Sulfoximine; D006861:Hydrogen Peroxide; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2; D005721:Glutamate-Cysteine Ligase; C024989:coenzyme Q10; D000111:Acetylcysteine