Combinatorial alanine substitution enables rapid optimization of cytochrome P450BM3 for selective hydroxylation of large substrates.
Department of Chemistry, University of Chicago, Chicago, IL 60637, USA.
Jared C
JC
Lewis
Simone M
SM
Mantovani
Yu
Y
Fu
Christopher D
CD
Snow
Russell S
RS
Komor
Chi-Huey
CH
Wong
Frances H
FH
Arnold
D000409:Alanine / Q000737:chemistry* / Q000378:metabolism; D000470:Alkaloids / Q000378:metabolism; D001410:Bacillus megaterium / Q000201:enzymology*; D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism*; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry* / Q000378:metabolism*; D006900:Hydroxylation; D009005:Monosaccharides / Q000378:metabolism; D009251:NADPH-Ferrihemoprotein Reductase / Q000737:chemistry* / Q000378:metabolism*; D013256:Steroids / Q000378:metabolism; D013379:Substrate Specificity
D000470:Alkaloids; D001426:Bacterial Proteins; D009005:Monosaccharides; D013256:Steroids; D003577:Cytochrome P-450 Enzyme System; D009251:NADPH-Ferrihemoprotein Reductase; C052423:flavocytochrome P450 BM3 monoxygenases; D000409:Alanine