Imaging blood vessels in the zebrafish.
Understanding on the mechanisms of vascular branching morphogenesis has become a subject of enormous scientific and clinical interest. Zebrafish, which have small, accessible, transparent embryos and larvae, provides a unique living animal model to facilitating high-resolution imaging on ubiquitous and deep localization of vessels within embryo development and also in adult tissues. In this chapter, we have summarized various methods for vessel imaging in zebrafish, including in situ hybridization for vascular-specific genes, resin injection- or dye injection-based vessel visualization, and alkaline phosphatase staining. We also described detail protocols for live imaging of vessels by microangiography or using various transgenic zebrafish lines.
Program in Genomics of Differentiation, National Institute of Child Health and Human Development, Bethesda, Maryland, USA.
Makoto
M
Kamei
Sumio
S
Isogai
Weijun
W
Pan
Brant M
BM
Weinstein
D000792:Angiography; D000818:Animals; D030801:Animals, Genetically Modified; D001808:Blood Vessels / Q000166:cytology*; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000378:metabolism; D017403:In Situ Hybridization; D059008:Time-Lapse Imaging; D015027:Zebrafish