Feasibility of diagnosing renal allograft dysfunction by oligonucleotide array: Gene expression profile correlates with histopathology.
BACKGROUND
Effective non-invasive monitoring method to tell histopathology is a big challenge in renal transplantation.


METHODS
We used 70-mer long oligonucleotide array with 449 immune related genes to determine gene expression profiles of peripheral blood mononuclear cells (PBMCs) under different immune status including stable renal function (TX), acute tubular necrosis (ATN), biopsy conformed acute rejection (AR), clinical rejection with pathology of borderline changes (BL), clinical rejection without biopsy proven/presumed rejection (PR) and renal dysfunction without rejection (NR).


RESULTS
Distinct molecular expression signatures in each group were found to correlate with histopathology. And we concluded that B cell chemokine CXCL13 and mast cell may play a role in renal allograft rejection through significant difference analysis and functional pathway analysis.


CONCLUSIONS
It provides a potential non-invasive method for monitoring renal allograft function and immune status of renal transplant recipients.
Kidney Disease Center, the First Affiliated Hospital, College of Medicine, Zhejiang University, China.
You-ying
YY
Mao
Hao
H
yang
Minmin
M
Wang
Wenhan
W
Peng
Qiang
Q
He
Zhang-fei
ZF
Shou
Hua
H
Jiang
Jianyong
J
Wu
Yong-qi
YQ
Fang
Hai-tao
HT
Dong
Jiang-hua
JH
Chen
D000293:Adolescent; D000328:Adult; D001402:B-Lymphocytes / Q000378:metabolism*; D001706:Biopsy; D054382:Chemokine CXCL13 / Q000235:genetics / Q000378:metabolism*; D005260:Female; D020869:Gene Expression Profiling; D006084:Graft Rejection / Q000175:diagnosis / Q000235:genetics / Q000473:pathology; D006801:Humans; D016030:Kidney Transplantation / Q000276:immunology / Q000473:pathology; D007683:Kidney Tubular Necrosis, Acute / Q000378:metabolism / Q000473:pathology; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008297:Male; D008407:Mast Cells / Q000378:metabolism; D008875:Middle Aged; D015166:Monitoring, Immunologic; D020411:Oligonucleotide Array Sequence Analysis
D054382:Chemokine CXCL13