Synthesis of histone H3 proteins by a thioacid capture ligation strategy.
The first application of thioacid capture ligation in protein synthesis is described. Two histone H3 proteins were synthesized in which a 30 min ligation reaction gave the protein products in good yields.
Division of Chemical Biology and Biotechnology, School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 63755, 1, Singapore.
Xiaohong
X
Zhang
Fupeng
F
Li
Chuan-Fa
CF
Liu
D002851:Chromatography, High Pressure Liquid; D004229:Dithiothreitol / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D006657:Histones / Q000138:chemical synthesis* / Q000737:chemistry; D007202:Indicators and Reagents / Q000737:chemistry; D010084:Oxidation-Reduction; D010720:Phosphines / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013438:Sulfhydryl Compounds / Q000737:chemistry*; D013997:Time Factors
D004229:Dithiothreitol; D006657:Histones; D007202:Indicators and Reagents; D010720:Phosphines; D013438:Sulfhydryl Compounds; C080938:tris(2-carboxyethyl)phosphine