[Osteoblastic early attachment onto the surface of bioadhesive peptide modified pure titanium].
OBJECTIVE
To investigate the long-term integrity and the biological function of interface between the bioadhesive peptide modified implant surface and peri-implant tissue.


METHODS
A short bioadhesive peptide containing Glycine-Tyrosine-Arginine-Glycine-Asparticacid-Serine (GYRGDS) sequence was immobilized onto the titanium implant surface by means of sol-gel coating technique and self-assembled monolayers (SAM). The chemical composition and organic functional groups on the titanium surfaces were characterized using XPS (X-ray photoelectron spectroscopy) and FTIR (Fourier transform infrared spectrometer). The adhesive strength and stability of osteoblasts on various implant surfaces were compared under flow condition.


RESULTS
The results showed that alkali/hot water aging treatment could apparently improve the content of -OH functional groups of titanium surface. The chemical reactive Ti-O-Ti bonding at the surface of titanium played a vital role in inducing the formation of organosilane SAM. GYRGDS peptide can be covalently grafted onto the surface of titanium by SAM technique. The resistance of freshly adherent osteoblasts to detachment by flow was shear time dependent. When the four groups were compared under the same flow stress condition (2.05 Pa) at three specific time spans (30 min, 1 h, 2 h), the cells retention rates in GYRGDS-grafted groups were 93.0%, 54.4%, 34.4% respectively and were much higher than those in non-coated groups.


CONCLUSIONS
It was suggested that GYRGDS might have positive effects on maintaining stability and adherence of cells onto the substrates under flow condition.
Department of Prosthodontics, West China School of Stomatology, Sichuan University, Chengdu 610041, China.
Yi-fan
YF
Chen
Yuan-jin
YJ
Huang
Guang-bao
GB
Song
Qian-bing
QB
Wan
Jian
J
Wang
Yong-lie
YL
Chao
D003738:Dental Cements; D010006:Osteoblasts / Q000502:physiology*; D010455:Peptides / Q000494:pharmacology*; D019736:Prostheses and Implants; D013499:Surface Properties; D014025:Titanium
D003738:Dental Cements; D010455:Peptides; D014025:Titanium