Humoral immune response to Aggregatibacter actinomycetemcomitans leukotoxin.
OBJECTIVE
Periodontal disease is an inflammatory condition caused by bacterial infections that result in loss of the tooth supporting tissue. The periodontal pathogens produce virulence factors with capacity to affect the host immune response. Aggregatibacter actinomycetemcomitans is a periodontal pathogen that produces a leukotoxin that specifically affects human leukocytes. The aims of the present study were to examine the presence and function of systemic antibodies to the leukotoxin.


METHODS
One hundred and ninety-seven middle-aged (57 ± 5 years) Swedes with well-documented periodontal status and medical factors related to cardiovascular diseases were studied. These data have been published previously. The serum samples were examined for the presence of leukotoxin antibodies by western blot and the capacity to neutralize leukotoxicity in an activity assay with leukotoxin and cultured leukemic cells.


RESULTS
The results showed a high prevalence (57%) of antibodies against A. actinomycetemcomitans leukotoxin in the analyzed population. These antibodies were correlated with leukotoxin neutralizing capacity as well as with the ELISA titers of A. actinomycetemcomitans-specific IgA and IgG. In addition, high levels of leukotoxin antibodies were correlated with increasing age, but not with periodontal disease parameters or cardiovascular risk factors.


CONCLUSIONS
Systemic antibodies against A. actionmycetemcomitans leukotoxin were common in this adult Swedish population. These antibodies might contribute to limit the systemic effects of the infection.
Division of Periodontology, Institute of Odontology, Umeå University, Umeå, Sweden.
M
M
Brage
A
A
Holmlund
A
A
Johansson
D016976:Aggregatibacter actinomycetemcomitans / Q000276:immunology*; D016301:Alveolar Bone Loss / Q000145:classification; D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology; D001427:Bacterial Toxins / Q000276:immunology*; D015153:Blotting, Western; D016022:Case-Control Studies; D045744:Cell Line, Tumor; D003603:Cytotoxins / Q000276:immunology*; D003774:Dental Plaque Index; D005098:Exotoxins / Q000276:immunology*; D006801:Humans; D006973:Hypertension / Q000150:complications; D056724:Immunity, Humoral / Q000276:immunology*; D007070:Immunoglobulin A / Q000097:blood / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D007166:Immunosuppressive Agents / Q000276:immunology*; D007948:Leukemia, Monocytic, Acute / Q000473:pathology; D008875:Middle Aged; D009203:Myocardial Infarction / Q000150:complications; D010512:Periodontal Index; D010518:Periodontitis / Q000382:microbiology
D000907:Antibodies, Bacterial; D001427:Bacterial Toxins; D003603:Cytotoxins; D005098:Exotoxins; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007166:Immunosuppressive Agents; C018579:leukotoxin