JULIDE: a software tool for 3D reconstruction and statistical analysis of autoradiographic mouse brain sections.
In this article we introduce JULIDE, a software toolkit developed to perform the 3D reconstruction, intensity normalization, volume standardization by 3D image registration and voxel-wise statistical analysis of autoradiographs of mouse brain sections. This software tool has been developed in the open-source ITK software framework and is freely available under a GPL license. The article presents the complete image processing chain from raw data acquisition to 3D statistical group analysis. Results of the group comparison in the context of a study on spatial learning are shown as an illustration of the data that can be obtained with this tool.
Signal Processing Laboratory (LTS5), Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.
Delphine
D
Ribes
Julia
J
Parafita
Rémi
R
Charrier
Fulvio
F
Magara
Pierre J
PJ
Magistretti
Jean-Philippe
JP
Thiran
D000818:Animals; D001345:Autoradiography / Q000379:methods; D001921:Brain / Q000033:anatomy & histology* / Q000502:physiology; D002250:Carbon Radioisotopes; D003847:Deoxyglucose / Q000378:metabolism; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008297:Male; D018782:Maze Learning / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D015203:Reproducibility of Results; D012984:Software
D002250:Carbon Radioisotopes; D003847:Deoxyglucose