Calibration of the injection volume for microinjection of Xenopus oocytes and embryos.
Microinjection of Xenopus oocytes or embryos with messenger RNA (mRNA) or DNA is a powerful technique for studying development. Before microinjection can be performed, the injection volume must be calibrated carefully. This protocol describes the calibration procedure for a pressure injector.
Hazel L
HL
Sive
Robert M
RM
Grainger
Richard M
RM
Harland
D000818:Animals; D004247:DNA / Q000008:administration & dosage* / Q000235:genetics; D004625:Embryo, Nonmammalian; D008845:Microinjections / Q000379:methods*; D009865:Oocytes; D012333:RNA, Messenger / Q000008:administration & dosage* / Q000235:genetics; D014981:Xenopus
D012333:RNA, Messenger; D004247:DNA