[The effect of the coded amino acids on the development of organotypic culture of the different genesis tissues from young and old rats].
The effect of 20 coded amino acids in concentration 10(-12) M was investigated in organotypic tissue culture on the cell proliferation development in myocardium, pancreas, brain cortex tissue (the tissues of meso-, ento- and ectodermal genesis accordingly) explants in 3- and 24-months old rats. The different effect was observed in the tissues of different genesis. The group of low molecular mass hydrophile amino acids with the charge chains stimulated the cell proliferation in the young and old rats. The other group of high molecular mass hydrophobe amino acids stimulated the cell proliferation in the young and old rats brain cortex. The number of active amino acids decreased significantly in the old rats--2 times in the entodermal tissues and 4 times in the meso- and ectodermal tissues, reflecting the disturbances of the amino acid transport by aging.
A V
AV
Smirnov
N I
NI
Chalisova
G A
GA
Ryzhak
E A
EA
Kontsevaia
D000367:Age Factors; D000375:Aging / Q000378:metabolism*; D000596:Amino Acids / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D000818:Animals; D017209:Apoptosis; D001921:Brain / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects*; D005815:Genetic Code; D008297:Male; D009206:Myocardium / Q000166:cytology / Q000378:metabolism; D009924:Organ Culture Techniques; D010179:Pancreas / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
D000596:Amino Acids