Immobilization and bioactivity evaluation of FGF-1 and FGF-2 on powdered silicon-doped hydroxyapatite and their scaffolds for bone tissue engineering.
Fibroblast growth factors (FGFs) are polypeptides that control the proliferation and differentiation of various cell types including osteoblasts. FGFs are also strong inducers of angiogenesis, necessary to obtain oxygen and nutrients during tissue repair. With the aim to incorporate these desirable FGF biological properties into bioceramics for bone repair, silicon substituted hydroxyapatites (Si-HA) were used as materials to immobilize bioactive FGF-1 and FGF-2. Thus, the binding of these growth factors to powdered Si-HA and Si-HA scaffolds was carried out efficiently in the present study and both FGFs maintained its biological activity on osteoblasts after its immobilization. The improvement of cell adhesion and proliferation onto Si-HA scaffolds suggests the potential utility of these FGF/scaffolds for bone tissue engineering.
Department of Biochemistry and Molecular Biology I, Faculty of Chemistry, Universidad Complutense, 28040, Madrid, Spain. mjfeito@bbm1.ucm.es
María José
MJ
Feito
Rosa María
RM
Lozano
María
M
Alcaide
Cecilia
C
Ramírez-Santillán
Daniel
D
Arcos
María
M
Vallet-Regí
María-Teresa
MT
Portolés
D001031:Apatites / Q000737:chemistry; D001842:Bone and Bones / Q000378:metabolism*; D002448:Cell Adhesion; D049109:Cell Proliferation; D017886:Durapatite / Q000737:chemistry*; D016220:Fibroblast Growth Factor 1 / Q000378:metabolism*; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism*; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D018613:Microscopy, Confocal / Q000379:methods; D009389:Neovascularization, Pathologic; D010006:Osteoblasts / Q000166:cytology; D010100:Oxygen / Q000737:chemistry; D011208:Powders; D017382:Reactive Oxygen Species; D012825:Silicon / Q000737:chemistry*; D023822:Tissue Engineering / Q000379:methods*
D001031:Apatites; D011208:Powders; D017382:Reactive Oxygen Species; D016222:Fibroblast Growth Factor 2; D016220:Fibroblast Growth Factor 1; D017886:Durapatite; D010100:Oxygen; D012825:Silicon