Identification of SUMOylated proteins in neuroblastoma cells after treatment with hydrogen peroxide or ascorbate.
The small ubiquitin-like modifier (SUMO) proteins have been implicated in the pathology of a number of diseases, including neurodegenerative diseases. The conjugation machinery for SUMOylation consists of a number of proteins which are redox sensitive. Here, under oxidative stress (100 μM hydrogen peroxide), antioxidant (100 μM ascorbate) or control conditions 169 proteins were identified by electrospray ionisation fourier transform ion cyclotron resonance mass spectrometry. The majority of these proteins (70%) were found to contain SUMOylation consensus sequences. From the remaining proteins a small number (12%) were found to contain possible SUMO interacting motifs. The proteins identified included DNA and RNA binding proteins, structural proteins and proteasomal proteins. Several of the proteins identified under oxidative stress conditions had previously been identified as SUMOylated proteins, thus validating the method presented.
School of Dentistry, University of Birmingham, Birmingham, B4 6NN, UK. melissa_grant@hotmail.com
Melissa M
MM
Grant
D000595:Amino Acid Sequence; D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology*; D045744:Cell Line, Tumor; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D013058:Mass Spectrometry; D009447:Neuroblastoma; D016877:Oxidants / Q000494:pharmacology*; D018384:Oxidative Stress; D011506:Proteins / Q000737:chemistry / Q000378:metabolism; D058207:Sumoylation / Q000187:drug effects
D000975:Antioxidants; D016877:Oxidants; D011506:Proteins; D006861:Hydrogen Peroxide; D001205:Ascorbic Acid