The integrative analysis of microRNA and mRNA expression in mouse uterus under delayed implantation and activation.
BACKGROUND
Delayed implantation is a developmental arrest at the blastocyst stage and a good model for embryo implantation. MicroRNAs (miRNAs) have been shown to be involved in mouse embryo implantation through regulating uterine gene expression. This study was to have an integrative analysis on global miRNA and mRNA expression in mouse uterus under delayed implantation and activation through Illumina sequencing.


RESULTS
By deep sequencing and analysis, we found that there are 20 miRNAs up-regulated and 42 miRNAs down-regulated at least 1.2 folds, and 268 genes up-regulated and 295 genes down-regulated at least 2 folds under activation compared to delayed implantation, respectively. Many different forms of editing in mature miRNAs are detected. The percentage of editing at positions 4 and 5 of mature miRNAs is significantly higher under delayed implantation than under activation. Although the number of miR-21 reference sequence under activation is slightly lower than that under delayed implantation, the total level of miR-21 under activation is higher than that under delayed implantation. Six novel miRNAs are predicted and confirmed. The target genes of significantly up-regulated miRNAs under activation are significantly enriched.


CONCLUSIONS
miRNA and mRNA expression patterns are closely related. The target genes of up-regulated miRNAs are significantly enriched. A high level of editing at positions 4 and 5 of mature miRNAs is detected under delayed implantation than under activation. Our data should be valuable for future study on delayed implantation.
Key Laboratory of the Ministry of Education for Cell Biology and Tumor Cell Engineering, School of Life Science, Xiamen University, Xiamen, China.
Ren-Wei
RW
Su
Wei
W
Lei
Ji-Long
JL
Liu
Zhi-Rong
ZR
Zhang
Bo
B
Jia
Xu-Hui
XH
Feng
Gang
G
Ren
Shi-Jun
SJ
Hu
Zeng-Ming
ZM
Yang
D000818:Animals; D002478:Cells, Cultured; D015536:Down-Regulation / Q000235:genetics; D010064:Embryo Implantation; D005260:Female; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D017403:In Situ Hybridization; D008297:Male; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D010052:Ovariectomy; D011247:Pregnancy; D012333:RNA, Messenger / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D015854:Up-Regulation / Q000235:genetics; D014599:Uterus / Q000166:cytology / Q000378:metabolism*
D035683:MicroRNAs; D012333:RNA, Messenger