Cellular uptake of protein-bound magnetic nanoparticles in pulsed magnetic field.
A method for fast delivery of proteins conjugated to superparamagnetic iron oxide nanoparticles (SPION) into mammalian cells by applying a strong magnetic field in pulses was proposed. Firstly, SPION were prepared from an alkaline solution of divalent and trivalent iron ions and covalently bound with protein through the activation of N-ethyl-N'-(3-dimethylaminopropyl) carbodiimide (EDC). After fluorescently labelling, the protein-nanoparticle conjugate was mixed with mammalian cell line and exposed to a pulsed magnetic field for short durations of few milliseconds. Results suggested that superparamagnetic nanoparticles were able to carry proteins into living cells immediately. Cellular internalization of the fluorescently labelled protein-nanoparticle conjugate was proved by the observation of cell fluorescence in a fluorescent microscopy, as well as cell analysis by a flow cytometer. We found that the cellular uptake was accomplished dominantly by the process of bombardment of magnetic nanoparticles.
Department of Chemical Engineering, Systems Biology and Tissue Engineering Research Center National Chung Cheng University, Chiayi, 621, Taiwan.
Chia-Haw
CH
Lee
Chao-Bin
CB
Chen
Ting-Hao
TH
Chung
Yu-Sheng
YS
Lin
Wen-Chien
WC
Lee
D004574:Electromagnetic Fields; D004705:Endocytosis; D005434:Flow Cytometry; D019793:Fluorescein / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D018613:Microscopy, Confocal; D058726:Nanoconjugates / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry* / Q000493:pharmacokinetics*; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000493:pharmacokinetics; D013054:Spectrophotometry, Atomic
D058185:Magnetite Nanoparticles; D058726:Nanoconjugates; D011506:Proteins; D012710:Serum Albumin, Bovine; D019793:Fluorescein