Use of N, N-diethyl-p-phenylenediamine sulphate for the spectrophotometric determination of some phenolic and amine drugs.
Spectrophotometric methods are proposed for the determination of drugs containing a phenol group [salbutamol sulphate (SLB), ritodrine hydrochloride (RTD), isoxsuprine hydrochloride (IXP)] and drugs containing an aromatic amine group [dapsone hydrochloride (DAP), sulfamethoxazole (SFM), and sulfadiazine (SFD)] in pharmaceutical dosage forms. The methods are based on coupling of N, N-diethyl-p-phenylenediamine sulphate with the drugs in the presence of KIO4 to give a green colored product (λmax at 670 nm) and a red colored product (λmax at 550 nm), respectively. Linear relationships with good correlation coefficients (0.9986-0.9996) were found between absorbance and the corresponding concentration of drugs in the range 1-7, 2-22, 1-17, 1.5-12, 2-25, and 2-21 μg mL-1 for SLB, RTD, IXP, DAP, SFM and SFD, respectively. Variable parameters such as temperature, reaction time and concentration of the reactants have been analyzed and optimized. The RSD of intra-day and inter-day studies was in the range of 0.2-1.0 and 0.4-1.0%, respectively. No interference was observed from common pharmaceutical adjuvants. The reliability and performance of the proposed methods was validated statistically; the percentage recovery ranged from 99.5 ± 0.1 to 99.9 ± 0.3%. Limits of detection were 0.14, 0.21, 0.51, 0.44, 0.33 and 0.37 μg mL-1 for SLB, RTD, IXP, DAP, SFM, and SFD, respectively.
Department of Studies in Chemistry, University of Mysore, Manasagangotri, Mysore-570006, India. saroj226@yahoo.com
Padmarajaiah
P
Nagaraja
Ashwinee Kumar
AK
Shrestha
Anantharaman
A
Shivakumar
Avinash K
AK
Gowda
D000588:Amines / Q000032:analysis* / Q000737:chemistry; D004304:Dosage Forms; D007202:Indicators and Reagents / Q000737:chemistry; D010504:Periodic Acid / Q000737:chemistry*; D010636:Phenols / Q000032:analysis* / Q000737:chemistry; D010655:Phenylenediamines / Q000737:chemistry*; D017680:Potassium Compounds / Q000737:chemistry*; D013053:Spectrophotometry / Q000379:methods*
D000588:Amines; D004304:Dosage Forms; D007202:Indicators and Reagents; D010636:Phenols; D010655:Phenylenediamines; D017680:Potassium Compounds; D010504:Periodic Acid; C013935:N,N-diethyl 4-phenylenediamine; C037192:potassium periodate