DNA cycle amplification device on magnetic microbeads for determination of thrombin based on graphene oxide enhancing signal-on electrochemiluminescence.
A novel and sensitive strategy which integrated a DNA cycle device onto magnetic microbeads (MB), amplifying the signal with graphene oxide (GO) and enhancing electrochemiluminescence (ECL) intensity, was developed and was further successfully applied to thrombin detection.
State Key Laboratory Base of Eco-chemical Engineering, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.
Yingshu
Y
Guo
Xueping
X
Jia
Shusheng
S
Zhang
D052157:Aptamers, Nucleotide / Q000737:chemistry*; D019187:Cadmium Compounds / Q000737:chemistry; D004247:DNA / Q000737:chemistry; D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods; D004566:Electrodes; D006046:Gold / Q000737:chemistry; D006108:Graphite / Q000737:chemistry*; D008163:Luminescent Measurements / Q000295:instrumentation / Q000379:methods*; D008280:Magnetics; D008863:Microspheres; D053758:Nanoparticles / Q000737:chemistry; D010087:Oxides / Q000737:chemistry; D013440:Sulfides / Q000737:chemistry; D013917:Thrombin / Q000032:analysis*
D052157:Aptamers, Nucleotide; D019187:Cadmium Compounds; D010087:Oxides; D013440:Sulfides; C034939:cadmium sulfide; D006046:Gold; D006108:Graphite; D004247:DNA; D013917:Thrombin