Association of paraoxonase gene polymorphisms with sperm parameters.
Paraoxonase (PON) is a high-density lipoprotein-associated enzyme that prevents low-density lipoprotein oxidation. PON proteins, localized in the seminiferous tubules and in spermatozoa, have been implicated in the pathogenesis of male infertility. In the present study, we sought to explore the contribution of the PON gene variants to sperm parameters. One hundred twenty oligospermic and 170 normozoospermic men were examined during infertility investigation. DNA was extracted from spermatozoa, and the PON1(L/M) 55, PON1(Q/R) 192, and PON2(S/C) 311 polymorphisms were genotyped by polymerase chain reaction and digestion with restriction enzymes. The analysis revealed that oligospermic men presented PON1 55L/L, PON1 192Q/Q, and PON2 311S/S genotypes less frequently than normozoospermic men (P < .01, P < .01, and P < .001, respectively), whereas the PON1 55M, PON1 192R, and PON2 311C alleles were significantly increased in oligospermic men (P < .004, P < .008, and P < .008, respectively). The presence of PON1 55L allele was associated with higher sperm motility in oligospermic men (P < .001), in normozoospermic men (P < .01), and in the total study population (P < .01), and the PON1 192Q allele was associated with higher sperm motility in oligospermic men (P < .01), in normozoospermic men (P < .04) and in the total study population (P < .03). On the other hand, the PON2 311S was associated with higher sperm concentration in oligospermic men (P < .03), in normozoospermic men (P < .008), and in the total study population (P < .001). In our series, the PON1 55M and PON1 192R alleles were associated with decreased sperm motility whereas the PON2 311C allele was associated with decreased concentration, supporting the significance of PON genes in semen quality.
Laboratory of Medical Genetics and Human Reproduction, Department of Obstetrics and Gynecology, Medical School, University of Ioannina, 45110 Ioannina, Greece.
Leandros A
LA
Lazaros
Nectaria V
NV
Xita
Elissavet G
EG
Hatzi
Apostolos I
AI
Kaponis
Theodoros J
TJ
Stefos
Nicolaos I
NI
Plachouras
Georgios V
GV
Makrydimas
Nicolaos V
NV
Sofikitis
Konstantinos A
KA
Zikopoulos
Ioannis A
IA
Georgiou
D000328:Adult; D043303:Aryldialkylphosphatase / Q000235:genetics*; D006801:Humans; D008297:Male; D009845:Oligospermia / Q000235:genetics*; D011110:Polymorphism, Genetic; D013076:Sperm Count; D013081:Sperm Motility
D043303:Aryldialkylphosphatase; C468406:PON1 protein, human; C468411:PON2 protein, human