[Effect of antisense KLF4 gene on the expression of vWF and PAI-1 in endothelium cells].
OBJECTIVE
To investigate the effect of antisense KLF4 (Krüppel-like factor 4) gene on the expression of vWF and PAI-1 in endothelial cells.


METHODS
Human umbilical vein endothelial cells (HUVEC) were isolated from umbilical vein and cultured in endothelial cell medium. The recombinant adenoviral plasmid carrying the antisense KLF4 gene was constructed by homologous recombination. KLF4, PAI-1 and vWF mRNAs and proteins expression were detected by real time-PCR, Western blot, and confocal laser microscopy.


RESULTS
Recombinant adenoviral plasmid carrying the antisense KLF4 gene (Ad-KLF4AS) was constructed successfully. Compared with the control Ad-GFP infection group, Ad-KLF4AS at a 200 MOI can down-regulate the expression of KLF4 gene in HUVEC (from 0.59 ± 0.01 to 0.44 ± 0.06) (P < 0.05), and increase vWF mRNA (from 1.04 ± 0.03 to 1.17 ± 0.05) and protein expression (P < 0.05). PAI-1 mRNA and protein of Ad-KLF4AS infection group was higher than that of Ad-GFP infection group. PAI-1 mRNA between the two groups had no significant difference (P > 0.05).


CONCLUSIONS
Down-regulation of KLF4 leads to increase in expression of vWF and PAI-1 in endothelial cells. KLF4 might play an important role in regulation of endothelial coagulant function.
Department of Hematology, the Second Hospital of Shanxi Medical University, Taiyuan 030001, China.
Rui-Juan
RJ
Zhang
Lin-Hua
LH
Yang
Yuan
Y
Zhang
Jian-Feng
JF
Zhou
Yang
Y
Cao
Cai-Hong
CH
Chen
D002478:Cells, Cultured; D015536:Down-Regulation; D042783:Endothelial Cells / Q000378:metabolism; D004727:Endothelium; D004730:Endothelium, Vascular / Q000378:metabolism; D006801:Humans; D000090062:Kruppel-Like Factor 4; D017395:Plasminogen Activator Inhibitor 1; D012333:RNA, Messenger / Q000235:genetics
C000715467:KLF4 protein, human; D000090062:Kruppel-Like Factor 4; D017395:Plasminogen Activator Inhibitor 1; D012333:RNA, Messenger