Duplication of cyb-3 (cyclin B3) suppresses sterility in the absence of mdf-1/MAD1 spindle assembly checkpoint component in Caenorhabditis elegans.
mdf-1/MAD1 is a conserved spindle assembly checkpoint component that is essential for the survival of Caenorhabditis elegans. Previously, using a dog-1(gk10)/FANCJ mutator strain, we have isolated a suppressor of mdf-1(gk2) sterility. This suppressor, named such-4, was demonstrated to be a tandem duplication that contained 62 putative protein coding genes. We apply here the recently developed Mos1-mediated single-copy insertion (MosSCI) method to study this copy number variation (CNV) in C. elegans and show that such-4 is caused by the duplication of a single gene cyb-3, illustrating the power of MosSCI-mediated single-gene duplications for uncovering gene dosage genetic interactions. Importantly, we show here, for the first time, that doubling the CYB-3 (Cyclin B3) dosage suppresses sterility in the absence of the essential spindle assembly checkpoint component MDF-1 without causing a delay in the onset of anaphase.
Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, BC, Canada.
Maja
M
Tarailo-Graovac
Jun
J
Wang
Domena
D
Tu
David L
DL
Baillie
Ann M
AM
Rose
Nansheng
N
Chen
D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics* / Q000378:metabolism; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism*; D002453:Cell Cycle / Q000502:physiology; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D019926:Cyclin B / Q000235:genetics / Q000378:metabolism*; D056915:DNA Copy Number Variations; D004265:DNA Helicases / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D018628:Gene Dosage; D018816:Genes, cdc; D005838:Genotype; D006801:Humans; D007246:Infertility / Q000235:genetics / Q000378:metabolism*; D008941:Spindle Apparatus / Q000378:metabolism; D019076:Transgenes; D019895:Transposases / Q000235:genetics / Q000378:metabolism
C491028:CYB-3 protein, C elegans; D029742:Caenorhabditis elegans Proteins; D018797:Cell Cycle Proteins; D019926:Cyclin B; D004268:DNA-Binding Proteins; C412839:MDF-1 protein, C elegans; C111652:mariner transposases; D019895:Transposases; D004265:DNA Helicases; C464789:Dog-1 protein, C elegans