Biochemical properties of free and immobilized Candida rugosa lipase onto Al2O3: a comparative study.
Lipase from Candida rugosa (CRL) was immobilized by physical adsorption onto Al(2)O(3). The immobilization yield was more than 95% during 30 min. The properties of free and immobilized enzyme were also searched and compared. The optimum pH was shifted from 7 to 8.5 by immobilization of the enzyme. The maximum activity of the free and the immobilized enzymes occured at 35 and 40°C, respectively.
Department of Chemistry, Faculty of Science, Trakya University, Edirne, Turkey. yesimyesiloglu@yahoo.com
Yeşim
Y
Yeşiloğlu
Latifşah
L
Şit
D000537:Aluminum Oxide / Q000737:chemistry*; D002175:Candida / Q000201:enzymology*; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008049:Lipase / Q000737:chemistry* / Q000378:metabolism*; D000069463:Olive Oil; D010938:Plant Oils / Q000378:metabolism; D013696:Temperature
D004800:Enzymes, Immobilized; D000069463:Olive Oil; D010938:Plant Oils; D008049:Lipase; D000537:Aluminum Oxide