alpha-Galactosidase from Bacillus megaterium VHM1 and its application in removal of flatulence-causing factors from soymilk.
A bacterial strain capable of producing extracellular alpha-galactosidase was isolated from sugar cane industrial waste soil sample. Microbiological, physiological, and biochemical studies revealed that isolate belonged to Bacillus sp,. Furthermore, 16S rDNA sequence analysis of new isolates was identified as Bacillus megaterium VHM1. The production of alpha-galactosidase was optimized by various physical culture conditions. Guar gum and yeast extract acted as the best carbon and nitrogen source, respectively for the production of alpha-galactosidase. The enzyme showed an optimum pH at 7.5 and was stable over a pH between 5 and 9. The enzyme was optimally active in 55degreesC and the enzyme was thermostable with half life of 120 minutes at 55 degrees C and lost their 90%, residual activity in 120 minutes at 60 degrees C. alpha-Galactosidase was strongly inhibited by Ag2, Cu2, and Hg2+ at 1mM concentration. The metal ions Fe2, Mn2+, and Mg2+ had no effect on alpha-galactosidase activity, Zn2+,Ni2+, and Ca2+ reduced the enzyme activity slightly. The B megaterium VHM1 enzyme treatment completely hydrolyzed flatulence-causing sugars of soymilk within one and half hour.
Department of Biochemistry, Gulbarga University, Gulbarga-585 106, Karnataka-585 106, India.
Aravind Goud G
AG
Patil
Praveen Kumar S
PK
K
Veerappa H
VH
Mulimani
Yaligara
Y
Veeranagouda
Kyoung
K
Lee
D000934:Antifoaming Agents / Q000737:chemistry / Q000378:metabolism*; D001410:Bacillus megaterium / Q000145:classification / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002241:Carbohydrates / Q000737:chemistry; D004795:Enzyme Stability; D005414:Flatulence; D008969:Molecular Sequence Data; D010802:Phylogeny; D031786:Saccharum / Q000382:microbiology; D041942:Soy Milk / Q000737:chemistry*; D000519:alpha-Galactosidase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
D000934:Antifoaming Agents; D001426:Bacterial Proteins; D002241:Carbohydrates; D000519:alpha-Galactosidase