Expression of osteopontin and type I collagen of hFOB 1.19 cells on sintered fluoridated hydroxyapatite composite bone graft materials.
OBJECTIVE
The biological effect of fluoridated hydroxyapatite (FHA) graft materials has been attributed to their fluoride ion content; but, only few studies have been conducted to explore the osteoblastic cellular response to physicochemical characteristics of them. We hypothesized that the effect of varied sintered FHA composites on osteoblastic behavior would attribute certain specified physicochemical characteristics of apatites.


METHODS
Sintered FHA composites were prepared by sintering method with varied gravity percentages of calcium fluoride and hydroxyapatite. Scanning electron microscopic, x-ray diffraction, and Fourier-transform infrared analysis were recorded. The human fetal-osteoblast (hFOB 1.19) cells were seeded on the apatites and tissue culture plates. Responses to the apatites were assessed in terms of osteopontin (OPN) and type I collagen, COL I, gene differentiation.


RESULTS
We observed the calcined hydroxyapatite (OHAp), sintered F- OHAps, and hydroxy fluorapatites (OH-FAps) with different physicochemical characteristics. The x-ray diffraction analysis showed sintered apatites to be fluorapatites. Otherwise, Fourier-transform infrared spectral patterns could differentiate the sintered F-OHAps from OH-FAps by the existence of OH, OH···F, or OH···F···OH bands. With ≤ 1 wt% CaF2 added, sintered F-OHAp composites expressed both OH and OH···F bands. With >1 wt% CaF2 added, sintered OH-FAp composites expressed both OH···F and OH···F···OH bands. Sintered F-OHAp composites could enhance OPN and COL I gene expression after 6-day culture (P ≤ 0.05). Otherwise, sintered OH-FAp composites inhibited the expression.


CONCLUSIONS
The results revealed that sintered F-OHAp composites with both OH and OH···F bands were bioactive bone graft materials.
College of Dental Science, Kaohsiung Medical University, Kaohsiung, Taiwan, Republic of China.
Cheng-Chei
CC
Wu
Shun-Te
ST
Huang
Hong-Ching
HC
Lin
Tien-Wen
TW
Tseng
Qui-Li
QL
Rao
Ming-Yu
MY
Chen
D000704:Analysis of Variance; D018786:Bone Substitutes / Q000737:chemistry*; D002124:Calcium Fluoride / Q000032:analysis; D002454:Cell Differentiation; D002460:Cell Line; D024042:Collagen Type I / Q000096:biosynthesis* / Q000235:genetics; D018360:Crystallography, X-Ray; D053686:Fetal Stem Cells / Q000166:cytology / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D006358:Hot Temperature; D006801:Humans; D006882:Hydroxyapatites / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*; D053495:Osteopontin / Q000096:biosynthesis* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017550:Spectroscopy, Fourier Transform Infrared; D018709:Statistics, Nonparametric
D018786:Bone Substitutes; D024042:Collagen Type I; D006882:Hydroxyapatites; D053495:Osteopontin; C028869:fluor-hydroxylapatite; D002124:Calcium Fluoride