Determination of photophysical parameters of red fluorescent protein mRFP1 under ultraviolet excitation by methods of laser fluorimetry.
We investigate photophysical processes that take place in macromolecules of a fluorescent protein mRFP1 under UV excitation [when the energy transfer in a localized donor-acceptor (LDA) pair, which is presented in the molecules of the protein, becomes apparent]. We used a special approach based on the fluorescence laser spectroscopy technique. The energy transfer rates in LDA pairs and photophysical parameters of fluorophores (chromophores) of three spectral forms, which coexist in the ensemble of the macromolecules of this protein, were determined under pulse UV laser excitation.
Department of Physics, M. V. Lomonosov Moscow State University, Moscow 119991, Russia. abanishev@gmail.com
Alexandr A
AA
Banishev
Evgeny A
EA
Shirshin
Victor V
VV
Fadeev
D000465:Algorithms; D004735:Energy Transfer; D031541:Fluorescence Resonance Energy Transfer / Q000295:instrumentation / Q000379:methods*; D007834:Lasers; D049408:Luminescent Agents / Q000737:chemistry*; D008164:Luminescent Proteins / Q000737:chemistry*; D008962:Models, Theoretical; D013997:Time Factors; D000097573:Red Fluorescent Protein
D049408:Luminescent Agents; D008164:Luminescent Proteins; D000097573:Red Fluorescent Protein