Microinjection of RNA and preparation of secreted proteins from Xenopus oocytes.
Microinjection of Xenopus oocytes or embryos with messenger RNA (mRNA) is a powerful technique for studying development, including protein expression during development. This protocol describes a method for the preparation of secreted (35)S-labeled proteins following mRNA microinjection into Xenopus oocytes.
Hazel L
HL
Sive
Robert M
RM
Grainger
Richard M
RM
Harland
D000818:Animals; D004625:Embryo, Nonmammalian; D008845:Microinjections / Q000379:methods*; D009865:Oocytes; D012313:RNA / Q000235:genetics* / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D013194:Staining and Labeling / Q000379:methods; D013462:Sulfur Radioisotopes / Q000378:metabolism; D014981:Xenopus
D011994:Recombinant Proteins; D013462:Sulfur Radioisotopes; D012313:RNA