Anisakis pegreffii: a quantitative fluorescence PCR assay for detection in situ.
To facilitate improved diagnosis and detection of the third stage larva (L3) of Anisakis pegreffii from the Minnan-Taiwan bank fishing ground in Taiwan Strait, a real-time PCR method for the detection in situ and differentiation was developed to amplify a region of the second internal transcribed spacer (ITS-2) of this parasite. The real-time PCR assay was capable of detecting 1/3 of a single L3 in 30 mg of marine fish tissue, and also exhibited a high level of specificity for A. pegreffii, no fluorescence signals were observed in other five major larval anisakid species found in commercial marine fishes caught in this fishing ground.
School of Life Sciences, Xiamen University, Xiamen, Fujian 361005, China.
Wenzhen
W
Fang
Fan
F
Liu
Shaolei
S
Zhang
Junhua
J
Lin
Shisan
S
Xu
Damin
D
Luo
D000818:Animals; D017129:Anisakiasis / Q000175:diagnosis / Q000469:parasitology / Q000662:veterinary*; D017163:Anisakis / Q000235:genetics / Q000302:isolation & purification*; D017931:DNA Primers; D015342:DNA Probes; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D005393:Fish Diseases / Q000175:diagnosis* / Q000469:parasitology; D005399:Fishes; D005456:Fluorescent Dyes; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D017931:DNA Primers; D015342:DNA Probes; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D005456:Fluorescent Dyes