Regulation of microRNA-145 by growth arrest and differentiation.
MicroRNA145 (miR145), a tumor suppressor miR, has been reported to inhibit growth of human cancer cells, to induce differentiation and to cause apoptosis, all conditions that result in growth arrest. In order to clarify the functional effects of miR145, we have investigated its expression in diverse conditions and different cell lines. Our results show that miR145 levels definitely increase in differentiating cells and also in growth-arrested cells, even in the absence of differentiation. Increased expression during differentiation sometimes occurs as a late event, suggesting that miR145 could be required either early or late during the differentiation process.
Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA 19107, USA.
Gaspare
G
La Rocca
Bin
B
Shi
Alessandra
A
Audia
Giovanna
G
Ferrari-Amorotti
Hestia S
HS
Mellert
Bruno
B
Calabretta
Steven B
SB
McMahon
Laura
L
Sepp-Lorenzino
Renato
R
Baserga
D017209:Apoptosis; D002087:Butyrates / Q000494:pharmacology; D022762:CCAAT-Enhancer-Binding Proteins / Q000378:metabolism; D002453:Cell Cycle; D002454:Cell Differentiation / Q000235:genetics*; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic; D006131:Growth Inhibitors / Q000032:analysis* / Q000494:pharmacology; D006801:Humans; D018021:Lithium Chloride / Q000494:pharmacology; D035683:MicroRNAs / Q000032:analysis* / Q000187:drug effects; D016271:Proto-Oncogene Proteins c-myc; D014212:Tretinoin / Q000494:pharmacology; D015854:Up-Regulation / Q000187:drug effects
D002087:Butyrates; D022762:CCAAT-Enhancer-Binding Proteins; C515848:CEBPA protein, human; D006131:Growth Inhibitors; C541010:MIRN145 microRNA, human; C489427:MYC protein, human; D035683:MicroRNAs; D016271:Proto-Oncogene Proteins c-myc; D014212:Tretinoin; D018021:Lithium Chloride