[Study of the expression of Aurora kinases in renal cell carcinoma].
OBJECTIVE
The Aurora kinase family plays a crucial role in the regulation of mitosis. Over-expression of Aurora A and B has been reported in many malignant tumors. The objective of this study was to analyze the expression of Aurora A and B in renal cell carcinoma (RCC) and its correlation with usual clinical and pathological parameters.


METHODS
In a retrospective study, have been studied the tumoral samples of 40 consecutive patients who had been operated between 2003 and 2006 for a renal tumor. RNA was extracted from frozen corresponding tumoral samples. Thirty-one samples were retained based on RNA quality. RT-PCR was done on each of these samples to assess the expression of Aurora A and B genes. Statistical analysis was performed using Chi-square test to compare Aurora A and B levels.


RESULTS
Median age was 65 years (35-82). Seven (22%) patients had nodal invasion and eight (26%) had distant metastases. Most of the tumors (74%) were grade 3 or 4. Eighteen patients (58%) had clear cell cancer histology, 12 (39%) had papillary histology, and one a Bellini type tumor. Aurora A overexpression was associated with lymph node invasion (p=0.001). Aurora B over-expression was associated with both nodal involvement (p=0.02) and histologic subtype (significantly over-expressed in clear cell tumors; p=0.001).


CONCLUSIONS
Aurora A and B were differentially over-expressed in clear cell RCC and primary tumors of patients with lymph node involvement.
Service d'urologie, hôpital Pontchaillou, CHU de Rennes, 2, rue Henri-Le-Guilloux, 35033 Rennes cedex, France.
R
R
Mathieu
J-J
JJ
Patard
N
N
Stock
N
N
Rioux-Leclercq
F
F
Guillé
P
P
Fergelot
K
K
Bensalah
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D064107:Aurora Kinase B; D064127:Aurora Kinases; D002292:Carcinoma, Renal Cell / Q000378:metabolism*; D006801:Humans; D007680:Kidney Neoplasms / Q000378:metabolism*; D008875:Middle Aged; D017346:Protein Serine-Threonine Kinases / Q000096:biosynthesis*; D012189:Retrospective Studies
C576921:AURKB protein, human; D064107:Aurora Kinase B; D064127:Aurora Kinases; D017346:Protein Serine-Threonine Kinases