Degradative potential of Stenotrophomonas strain HPC383 having genes homologous to dmp operon.
A strain, Stenotrophomonas HPC383 is isolated from effluent treatment plant treating wastewater from pesticide industry; degrades various aromatic compounds (cresols, phenol, catechol, 4methyl-catechol and hydroquinone) and crude oil, as determined through HPLC and GC analysis. Culture HPC383 could degrade (%) various compounds (1 mM) from a mixture: phenol - 99, p-cresol - 100, 4-methylcatechol - 96 and hydroquinone - 43 within 48 h of incubation, whereas it took 7 days to degrade 94% of 0.5% crude oil. Gene locus dmpN, to identify phenol degrading capacity was determined by PCR followed by southern analysis. The sequenced DNA fragment exhibited 99% sequence similarity to phenol hydroxylase gene from Arthrobacter sp. W1 (FJ610336). Amino acid sequence analysis of phenol hydroxylase reveals it to belong to high-Ks (affinity constant) group. Application of HPC383 in bioremediation of aquatic and terrestrial sites contaminated with petrochemical has been suggested.
Environmental Genomics Unit, National Environmental Engineering Research Institute (NEERI), CSIR, Nehru Marg, Nagpur (MH), India.
Vinita
V
Verma
Sajan C
SC
Raju
Atya
A
Kapley
Vipin Chandra
VC
Kalia
Gajanan S
GS
Kanade
Hatim F
HF
Daginawala
Hemant J
HJ
Purohit
D001483:Base Sequence; D001673:Biodegradation, Environmental; D006841:Hydrocarbons, Aromatic / Q000378:metabolism*; D006899:Mixed Function Oxygenases / Q000235:genetics*; D008969:Molecular Sequence Data; D009876:Operon / Q000235:genetics; D010575:Pesticides / Q000378:metabolism*; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D020588:Stenotrophomonas / Q000737:chemistry / Q000145:classification* / Q000502:physiology*; D014874:Water Pollutants, Chemical / Q000378:metabolism*; D018508:Water Purification / Q000379:methods*
D006841:Hydrocarbons, Aromatic; D010575:Pesticides; D014874:Water Pollutants, Chemical; D006899:Mixed Function Oxygenases; C022336:phenol 2-monooxygenase