Simultaneous dual molecular contrasts provided by the absorbed photons in photoacoustic microscopy.
We investigated the feasibility of simultaneously imaging two distinctive molecular contrasts provided by the absorbed photons in biological tissues with a single light source. The molecular contrasts are based on two physical effects induced by the absorbed photons: photoacoustics (PA) and autofluorescence (AF). In an integrated multimodal imaging system, the PA and AF signals were detected by a high-sensitivity ultrasonic transducer and an avalanche photodetector, respectively. The system was tested by imaging ocular tissue samples, including the retinal pigment epithelium and the ciliary body. The acquired images provided information on the spatial distributions of melanin and lipofuscin in these samples.
Department of Ophthalmology, Keck School of Medicine, University of Southern California, 1450 San Pablo Street, Los Angeles, California 90033, USA.
Xiangyang
X
Zhang
Minshan
M
Jiang
Amani A
AA
Fawzi
Xiang
X
Li
K Kirk
KK
Shung
Carmen A
CA
Puliafito
Hao F
HF
Zhang
Shuliang
S
Jiao
D000042:Absorption; D000162:Acoustics; D000818:Animals; D006801:Humans; D008062:Lipofuscin / Q000378:metabolism; D008543:Melanins / Q000378:metabolism; D008853:Microscopy / Q000379:methods*; D057054:Molecular Imaging / Q000379:methods*; D055620:Optical Phenomena; D017785:Photons; D055213:Retinal Pigment Epithelium / Q000378:metabolism
D008062:Lipofuscin; D008543:Melanins