Morphological nuclear integrity of sperm cells is associated with preimplantation genetic aneuploidy screening cycle outcomes.
OBJECTIVE
To examine the effect of sperm morphology on embryo development at the chromosomal level.


METHODS
Prospective study.


METHODS
Assisted fertilization center.


METHODS
Couples who underwent IVF-PGS cycle, as a result of advanced maternal age, were randomly allocated into two groups: intracytoplasmic sperm injection (ICSI; n = 60) or intracytoplasmic morphologically selected sperm injection (IMSI; n = 60).


METHODS
IVF in conjunction with preimplantation genetic screening (PGS).


METHODS
Sperm nuclear morphology at high-magnification ICSI and incidence of aneuploidy in derived embryo.


RESULTS
There was a significantly increased incidence for sex chromosome aneuploidy in ICSI embryos when compared with IMSI embryos (23.5% vs. 15.0%, respectively). High-magnification sperm selection was associated with a significantly lower risk of sex chromosome abnormalities (odds ratio [OR], 0.57; confidence interval [CI], 0.37-0.90). The incidence of chaotic embryos was also significantly higher with the ICSI procedure (27.5% vs. 18.8%), while the IMSI procedure was associated with a significantly lower risk of chaotic embryos (OR, 0.64; CI, 0.43-0.96). Moreover, the cycle cancellation rate was significantly higher in ICSI cycles (11.8% vs. 2.5%). High-magnification sperm selection was a significant predictor of the likelihood of cycle cancellation (OR, 0.26; CI, 0.11-0.62).


CONCLUSIONS
Spermatozoa free of nuclear morphological malformations were found to be significantly associated with the lower incidence of aneuploidy in derived embryos, resulting in lower rates of cycle cancellation.
Fertility-Assisted Fertilization Centre, São Paulo, Brazil.
Rita de Cássia S
Rde C
Figueira
Daniela P A F
DP
Braga
Amanda S
AS
Setti
Assumpto
A
Iaconelli
Edson
E
Borges
D000328:Adult; D000782:Aneuploidy; D001755:Blastocyst / Q000502:physiology; D002467:Cell Nucleus / Q000502:physiology; D047108:Embryonic Development / Q000235:genetics; D005260:Female; D005820:Genetic Testing / Q000379:methods* / Q000706:statistics & numerical data; D006801:Humans; D015994:Incidence; D008297:Male; D008423:Maternal Age; D008875:Middle Aged; D011237:Predictive Value of Tests; D011247:Pregnancy; D018873:Pregnancy Rate; D019836:Preimplantation Diagnosis / Q000379:methods* / Q000706:statistics & numerical data; D011446:Prospective Studies; D012307:Risk Factors; D012729:Sex Chromosome Aberrations / Q000706:statistics & numerical data*; D020554:Sperm Injections, Intracytoplasmic; D013094:Spermatozoa / Q000166:cytology* / Q000502:physiology