Computational analysis and structure predictions of CHH-related peptides from Litopenaeus vannamei.
The crustaceans produce several related peptides that belong to the crustacean hyperglycemic hormone (CHH) family. While these peptides have similar amino acid sequences, they have diverse biological functions that must arise, in part, from differences in the 3D shape of these peptides. However, it is generally accepted that peptides with a high degree of sequence similarity also have a similar 3-D structure. We used the solution structure of one peptide in the crustacean hyperglycemic hormone family, the molt-inhibiting hormone of the kuruma prawn (Marsupenaeus japonicus), to predict the shape of the five known peptides related to CHH in the Pacific white shrimp, Litopenaeus vannamei. The high similarity of the 3-D structures of these peptides suggests a common fold for the entire family. Nevertheless, minor differences in the shape of these peptides were observed, which may be the basis for their different biological properties.
Department of Biology, University of Central Florida, Orlando, FL 32816, USA. pganji@emory.edu
G Purna Chandra
GP
Nagaraju
N Siva
NS
Kumari
G L V
GL
Prasad
B Reddya
BR
Naik
D W
DW
Borst
D000107:Acetylation; D000595:Amino Acid Sequence; D000818:Animals; D060829:Arthropod Proteins; D019295:Computational Biology / Q000379:methods*; D003553:Cystine / Q000737:chemistry; D005229:Fatty Acids, Monounsaturated / Q000378:metabolism; D006031:Glycosylation; D057927:Hydrophobic and Hydrophilic Interactions; D007447:Invertebrate Hormones; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D009479:Neuropeptides / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D033561:Penaeidae / Q000737:chemistry* / Q000235:genetics; D010766:Phosphorylation; D011487:Protein Conformation; D011499:Protein Processing, Post-Translational; D017433:Protein Structure, Secondary; D017386:Sequence Homology, Amino Acid
D060829:Arthropod Proteins; D005229:Fatty Acids, Monounsaturated; D007447:Invertebrate Hormones; D009419:Nerve Tissue Proteins; D009479:Neuropeptides; C015947:hyperglycemic hormone, crustacean; D003553:Cystine; C054211:9-tetradecenoic acid