[Effects of histone deacetylase inhibitor on the expression of angiogenesis related factors in Kasumi-1 leukemic cell line].
OBJECTIVE
To investigate the effects of two histone deacetylase (HDAC) inhibitors, valproic acid (VPA) and TSA, on the expression of vascular endothelial growth factor (VEGF) and its receptor KDR of the leukemia cell line Kasumi-1 cells, and to explore their potential mechanism in leukemia angiogenesis.


METHODS
Kasumi-1 cells were treated with VPA and TSA at different concentrations for 3 days. The mRNA and protein expression levels of VEGF and KDR were determined by semi-quantitative RT-PCR and Western blot, and the bFGF mRNA by semi-quantitative RT-PCR.


RESULTS
As compared with that of control groups, VPA at 3 mmol/L downregulated the VEGF mRNA expression level for VEGF(121) from 0.632 ± 0.014 to 0.034 ± 0.004 and for VEGF(165) from 0.526 ± 0.021 to 0.015 ± 0.001, for KDR mRNA from 0.258 ± 0.034 to 0.038 ± 0.000, and for bFGF mRNA from 0.228 ± 0.017 to 0.086 ± 0.015. TSA downregulated the VEGF mRNA and KDR mRNA at concentration of 100 nmol/L, but its effect on bFGF mRNA only at higher concentration.


CONCLUSIONS
HDAC inhibitors might inhibit the leukemia angiogenesis by regulating the expression of VEGF and its recptor.
Department of Hematology, Affiliated Hospital of Chengde Medical College, Chengde 067000, China.
Cui-Min
CM
Zhu
Zhi-Hua
ZH
Zhang
Feng-Yun
FY
Jiang
Bao-Qin
BQ
Liu
Lei
L
Zhao
Wen-Liang
WL
Tian
Li-Na
LN
Yan
Zhi-Qiang
ZQ
Liang
Chang-Lai
CL
Hao
D043925:Angiogenesis Inducing Agents; D002460:Cell Line; D056572:Histone Deacetylase Inhibitors / Q000494:pharmacology; D006801:Humans; D012333:RNA, Messenger / Q000235:genetics; D014635:Valproic Acid / Q000494:pharmacology; D042461:Vascular Endothelial Growth Factor A
D043925:Angiogenesis Inducing Agents; D056572:Histone Deacetylase Inhibitors; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A; D014635:Valproic Acid