Rubisco activity in Mediterranean species is regulated by the chloroplastic CO2 concentration under water stress.
Water stress decreases the availability of the gaseous substrate for ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) by decreasing leaf conductance to CO(2). In spite of limiting photosynthetic carbon assimilation, especially in those environments where drought is the predominant factor affecting plant growth and yield, the effects of water deprivation on the mechanisms that control Rubisco activity are unclear. In the present study, 11 Mediterranean species, representing different growth forms, were subject to increasing levels of drought stress, the most severe one followed by rewatering. The results confirmed species-specific patterns in the decrease in the initial activity and activation state of Rubisco as drought stress and leaf dehydration intensified. Nevertheless, all species followed roughly the same trend when Rubisco activity was related to stomatal conductance (g(s)) and chloroplastic CO(2) concentration (C(c)), suggesting that deactivation of Rubisco sites could be induced by low C(c), as a result of water stress. The threshold level of C(c) that triggered Rubisco deactivation was dependent on leaf characteristics and was related to the maximum attained for each species under non-stressing conditions. Those species adapted to low C(c) were more capable of maintaining active Rubisco as drought stress intensified.
Grup de Recerca en Biologia de les Plantes en Condicions Mediterrànies, IMEDEA-Universitat de les Illes Balears, Carretera de Valldemossa Km 7.5, 07122 Palma de Mallorca, Spain. jeroni.galmes@uib.cat
Jeroni
J
Galmés
Miquel
M
Ribas-Carbó
Hipólito
H
Medrano
Jaume
J
Flexas
D002245:Carbon Dioxide / Q000378:metabolism*; D002736:Chloroplasts / Q000378:metabolism*; D055864:Droughts; D018506:Gene Expression Regulation, Plant; D019083:Mediterranean Region; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D010944:Plants / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D012273:Ribulose-Bisphosphate Carboxylase / Q000235:genetics / Q000378:metabolism*; D014867:Water / Q000378:metabolism*
D010940:Plant Proteins; D014867:Water; D002245:Carbon Dioxide; D012273:Ribulose-Bisphosphate Carboxylase