Proteomic analysis of autoantigens associated with systemic lupus erythematosus: Anti-aldolase A antibody as a potential marker of lupus nephritis.
To screen for autoantibodies associated with systemic lupus erythematosus (SLE), we used proteomic approaches combining 2-D PAGE and Western blot analysis, followed by protein identification by LC-MS/MS analysis, resulting in the identification of aldolase A as a novel autoantigen in SLE. ELISA showed the prevalence of anti-aldolase A antibodies to be 29.3% in SLE, 8.2% in rheumatoid arthritis, 18.1% in polymyositis and absent in healthy controls. Furthermore, 43.4% of SLE patients suffering from nephritis showed anti-aldolase A autoantibodies, which was significantly higher than the prevalence for those without nephritis (11.1%). In lupus nephritis, there are few reliable diagnostic methods, other than urinalysis. Therefore, these results indicate that autoantibodies against aldolase A may serve as an alternative clinical biomarker of SLE associated with nephritis.
Functional Proteomics Group, Osaka University Graduate School of Frontier Biosciences, Osaka, Japan.
Satoshi
S
Serada
Minoru
M
Fujimoto
Tsuyoshi
T
Takahashi
Ping
P
He
Akio
A
Hayashi
Toshio
T
Tanaka
Keisuke
K
Hagihara
Tomoki
T
Yamadori
Masao
M
Mochizuki
Naoko
N
Norioka
Shigemi
S
Norioka
Ichiro
I
Kawase
Tetsuji
T
Naka