(Coixan polysaccharide)-graft-polyethylenimine folate for tumor-targeted gene delivery.
CP-PEI-FA was prepared as an effective vector for in vitro and in vivo tumor-targeted gene delivery. The structures of the polymers were characterized, and their DNA condensation capability, particle sizes, zeta potentials, cytotoxicity and in vitro/in vivo transfection were examined. The cytotoxicity of CP-PEI-FA was significantly lower than that of PEI 25 kDa and close to that of PEI 1200. The in vitro transfection of CP-PEI-FA was tested in C6 and HeLa cells (FR-positive cells) and A549 cells (FR-negative cells). CP-PEI-FA showed a high targeting specificity and good gene transfection efficiency in FR-positive cells. These results indicate that CP-PEI-FA is a safe and effective polyplex-forming agent for both in vitro and in vivo transfection of plasmid DNA.
Institute of Chemical Biology and Pharmaceutical Chemistry, Zhejiang University, Hangzhou 310028, China.
Qiying
Q
Jiang
Peihua
P
Shi
Chunyan
C
Li
Qingqing
Q
Wang
Fujian
F
Xu
Wantai
W
Yang
Guping
G
Tang
D000818:Animals; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D004247:DNA / Q000235:genetics; D024202:Electrophoretic Mobility Shift Assay; D005492:Folic Acid / Q000138:chemical synthesis / Q000737:chemistry* / Q000633:toxicity; D018014:Gene Transfer Techniques; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008807:Mice, Inbred BALB C; D008856:Microscopy, Fluorescence; D009369:Neoplasms / Q000378:metabolism* / Q000473:pathology; D056951:Photoelectron Spectroscopy; D010957:Plasmids / Q000235:genetics; D011094:Polyethyleneimine / Q000138:chemical synthesis / Q000737:chemistry* / Q000633:toxicity; D011134:Polysaccharides / Q000138:chemical synthesis / Q000737:chemistry* / Q000633:toxicity; D051381:Rats; D013056:Spectrophotometry, Ultraviolet; D014162:Transfection
D011134:Polysaccharides; C048343:coixan; D011094:Polyethyleneimine; D004247:DNA; D005492:Folic Acid