Development of a universal plate-agglutination test for detecting Haemophilus parasuis.
Due to the serovar diversity in Haemophilus (H.) parasuis, it is difficult to develop a universal serological method for detection of this pathogen. Here, we report a universal plate-agglutination test for detecting H. parasuis. Diagnostic antisera were prepared by mixing antisera of serovars 4, 5, 12, 13 and 14 in the optimized ratio. The results of the plate-agglutination test showed that the diagnostic antisera could agglutinate with all 15 reference strains of H. parasuis and 74/75 clinical isolates. Further, the specificity of the method was validated with 22 bacterial strains from 12 related species.
College of Life Science and Technology, Southwest University for Nationalities, Chengdu, 610041 China.
Dingqian
D
Guo
Cheng
C
Tang
Quan
Q
Hai
Guoqing
G
Shao
Hua
H
Yue
D000372:Agglutination Tests / Q000379:methods*; D000818:Animals; D003429:Cross Reactions; D044137:Haemophilus parasuis / Q000302:isolation & purification / Q000502:physiology*; D007106:Immune Sera / Q000378:metabolism*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D007106:Immune Sera