Production of fumonisins B2 and B4 in Tolypocladium species.
Tolypocladium inflatum is known primarily for its production of the cyclosporines that are used as an immunosuppressive drug. However, we report here the production of the carcinogenic fumonisins B(2) and B(4) by this biotechnologically relevant fungal genus. These mycotoxins were detected in 11 strains tested from three species: Tolypocladium inflatum, T. cylindrosporum, and T. geodes. Production of fumonisins by Fusarium spp. and Aspergillus niger is highly medium- and temperature-dependent, so the effect of these parameters on fumonisin production by three T. inflatum strains was studied. Maximum production was achieved on media with high sugar content incubated at 25-30°C. Since these results demonstrate that fumonisin production could be widespread within the genus Tolypocladium, the potential contamination of commercial cyclosporine preparations with fumonisins needs to be investigated.
Center for Microbial Biotechnology, Department of Systems Biology, Technical University of Denmark, Søltofts Plads 221, 2800 Kgs, Lyngby, Denmark. jemo@bio.dtu.dk
Jesper Mølgaard
JM
Mogensen
Kirsten Amalie
KA
Møller
Pernille
P
von Freiesleben
Roman
R
Labuda
Elisabeth
E
Varga
Michael
M
Sulyok
Alena
A
Kubátová
Ulf
U
Thrane
Birgitte
B
Andersen
Kristian Fog
KF
Nielsen
D001234:Aspergillus niger / Q000378:metabolism; D037341:Fumonisins / Q000737:chemistry / Q000378:metabolism*; D006999:Hypocreales / Q000378:metabolism*; D009183:Mycotoxins / Q000096:biosynthesis* / Q000737:chemistry; D013696:Temperature
D037341:Fumonisins; D009183:Mycotoxins; C056934:fumonisin B2; C076287:fumonisin B4