title: Differential expression and function of the caveolin-1 gene in non-small cell lung carcinoma.
abstract: This study was designed to clarify the function of caveolin-1 (Cav-1) in the development of lung cancer by investigating the mutation and protein expression of the Cav-1 gene in non-small cell lung carcinoma (NSCLC). Quantum dot immunofluorescence histochemistry was used to evaluate Cav-1 protein expression and subcellular localization in the lung cancer tissue microarray including 140 cases of lung cancer and 20 cases of non-cancerous lung tissue. Mutation of the Cav-1 gene in exon 1 and exon 3 was detected by polymerase chain reaction-single strand conformation polymorphism and sequencing. The positive rates of Cav-1 expression were 49.3% (69/140) in NSCLC group, significantly lower than the 100% (20/20) rate in the control group. Adenocarcinomas (16.7%), adenosquamous carcinomas (38.4%), squamous cell carcinomas (67.1%) and large cell lung cancers (66.7%) displayed Cav-1 positive staining, suggesting a gradient of Cav-1 expression according to tumor histotype-related aggressiveness. High-expression of Cav-1 protein was statistically correlated with pathologic TNM stage and lymph node metastasis. No mutation could be detected in exon 1 and exon 3 from all Cav-1 protein negative expression of NSCLC samples. Cav-1 immunoreactivity in lung cancer is histotype-dependent, increased Cav-1 expression indicates the malignant progression and high invasion features of NSCLCs. Deregulation of Cav-1 expression in NSCLCs may not correlate with mutation.
authors.affiliation: Department of Pathology, School of Basic Medicine Science, Wuhan University, Donghu Road 115, Wuhan 430071, PR China. chenhlwhu@gmail.com
authors.forename: Hong Lei
authors.initials: HL
authors.lastname: Chen
authors.forename: Li Fang
authors.initials: LF
authors.lastname: Fan
authors.forename: Jun
authors.initials: J
authors.lastname: Gao
authors.forename: Jing Ping
authors.initials: JP
authors.lastname: Ouyang
authors.forename: Yu Xia
authors.initials: YX
authors.lastname: Zhang
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D016022:Case-Control Studies; D051242:Caveolin 1 / Q000235:genetics* / Q000502:physiology*; D004252:DNA Mutational Analysis / Q000379:methods; D018450:Disease Progression; D005260:Female; D019085:Fluorescent Antibody Technique, Direct; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D008297:Male; D008875:Middle Aged; D018807:Polymorphism, Single-Stranded Conformational; D046888:Tissue Array Analysis
chemical_list: D051242:Caveolin 1