title: Light regulation of protein dimerization and kinase activity in living cells using photocaged rapamycin and engineered FKBP.
abstract: We developed a new system for light-induced protein dimerization in living cells using a photocaged analogue of rapamycin together with an engineered rapamycin binding domain. Using focal adhesion kinase as a target, we demonstrated successful light-mediated regulation of protein interaction and localization in living cells. Modification of this approach enabled light-triggered activation of a protein kinase and initiation of kinase-induced phenotypic changes in vivo.
authors.affiliation: Department of Pharmacology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, United States.
authors.forename: Andrei V
authors.initials: AV
authors.lastname: Karginov
authors.forename: Yan
authors.initials: Y
authors.lastname: Zou
authors.forename: David
authors.initials: D
authors.lastname: Shirvanyants
authors.forename: Pradeep
authors.initials: P
authors.lastname: Kota
authors.forename: Nikolay V
authors.initials: NV
authors.lastname: Dokholyan
authors.forename: Douglas D
authors.initials: DD
authors.lastname: Young
authors.forename: Klaus M
authors.initials: KM
authors.lastname: Hahn
authors.forename: Alexander
authors.initials: A
authors.lastname: Deiters
mesh_terms: D019281:Dimerization; D051416:Focal Adhesion Protein-Tyrosine Kinases / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D008027:Light; D008958:Models, Molecular; D056004:Molecular Dynamics Simulation; D020123:Sirolimus / Q000737:chemistry*; D022021:Tacrolimus Binding Proteins / Q000737:chemistry / Q000378:metabolism*
chemical_list: D051416:Focal Adhesion Protein-Tyrosine Kinases; D022021:Tacrolimus Binding Proteins; D020123:Sirolimus