title: [Effects of insulin on production of NO and NOS mRNA expression in bovine aortic endothelial cells].
abstract: OBJECTIVE
  In order to explore the regulation of the production of nitric oxide (NO) and the expression of nitric oxide synthase (NOS) gene in bovine aortic endothelial cells (BAEC) by insulin.
  
  
  METHODS
  The NO2- which is the production of NO in the culture medium was determined based on Griess reaction. Quantitative RT/PCR technique was performed to quantitate the levels of NOS mRNA in BAEC.
  
  
  RESULTS
  (1) Insulin neither affected BAEC proliferation, nor had cytotoxic effect. (2) Over a concentration range of 1-15 microg/ ml, insulin stimulation of BAEC resulted in a dose-dependent production of NO in the culture supernatants 2 hours later, with a maximum at 12 hours and this effect was completely blocked by L-NAME. (3) NOS mRNA was slightly greater in BAEC after exposed to insulin, but without statistical significance.
  
  
  CONCLUSIONS
  Since the endothelial cell proliferation and the expression of NOS mRNA was not affected with insulin, our data suggested that the insulin-induced NO production was mediated via the activation of NOS activity.
authors.affiliation: Department of Cardiology, Renji Hospital, Shanghai Second Medical University, Shanghai 200001.
authors.forename: H L
authors.initials: HL
authors.lastname: Li
authors.forename: D J
authors.initials: DJ
authors.lastname: Huang
authors.forename: X J
authors.initials: XJ
authors.lastname: Zhang
authors.forename: Q
authors.initials: Q
authors.lastname: Zhao
authors.forename: A J
authors.initials: AJ
authors.lastname: Wan
mesh_terms: D000818:Animals; D001011:Aorta / Q000166:cytology; D002417:Cattle; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000187:drug effects* / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology; D007328:Insulin / Q000494:pharmacology*; D009569:Nitric Oxide / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D007328:Insulin; D012333:RNA, Messenger; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase