title: Decorating microbes: surface display of proteins on Escherichia coli.
abstract: Bacterial surface display entails the presentation of recombinant proteins or peptides on the surface of bacterial cells. Escherichia coli is the most frequently used bacterial host for surface display and, as such, a variety of E. coli display systems have been described that primarily promote the surface exposure of peptides and small proteins. By contrast, display systems based on autotransporter proteins (ATs) and ice nucleation protein (INP) are excellent systems for the display of large and complex proteins, and are therefore of considerable biotechnological relevance. Here, we review recent advances in AT and INP-mediated display and their biotechnological applications. Additionally, we discuss several promising alternative display methods, as well as novel bacterial host organisms.
authors.affiliation: Laboratory of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands.
authors.forename: Edwin
authors.initials: E
authors.lastname: van Bloois
authors.forename: Remko T
authors.initials: RT
authors.lastname: Winter
authors.forename: Harald
authors.initials: H
authors.lastname: Kolmar
authors.forename: Marco W
authors.initials: MW
authors.lastname: Fraaije
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism*; D001709:Biotechnology / Q000379:methods*; D004798:Enzymes / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism*; D019151:Peptide Library; D010455:Peptides / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D021381:Protein Transport; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D001425:Bacterial Outer Membrane Proteins; D004798:Enzymes; D019151:Peptide Library; D010455:Peptides; D011993:Recombinant Fusion Proteins; C063113:ice nucleation protein