title: Changes in mouse whole saliva soluble proteome induced by tannin-enriched diet.
abstract: BACKGROUND
  Previous studies suggested that dietary tannin ingestion may induce changes in mouse salivary proteins in addition to the primarily studied proline-rich proteins (PRPs). The aim of the present study was to determine the protein expression changes induced by condensed tannin intake on the fraction of mouse whole salivary proteins that are unable to form insoluble tannin-protein complexes. Two-dimensional polyacrylamide gel electrophoresis protein separation was used, followed by protein identification by mass spectrometry.
  
  
  RESULTS
  Fifty-seven protein spots were excised from control group gels, and 21 different proteins were identified. With tannin consumption, the expression levels of one α-amylase isoform and one unidentified protein increased, whereas acidic mammalian chitinase and Muc10 decreased. Additionally, two basic spots that stained pink with Coomassie Brilliant Blue R-250 were newly observed, suggesting that some induced PRPs may remain uncomplexed or form soluble complexes with tannins.
  
  
  CONCLUSIONS
  This proteomic analysis provides evidence that other salivary proteins, in addition to tannin-precipitating proteins, are affected by tannin ingestion. Changes in the expression levels of the acidic mammalian chitinase precursor and in one of the 14 salivary α-amylase isoforms underscores the need to further investigate their role in tannin ingestion.
authors.affiliation: ITQB-Instituto de Tecnologia Química e Biológica, Oeiras, Portugal. varela@itqb.unl.pt.
authors.forename: Elsa
authors.initials: E
authors.lastname: Lamy
authors.forename: Gonçalo
authors.initials: G
authors.lastname: Graça
authors.forename: Gonçalo
authors.initials: G
authors.lastname: da Costa
authors.forename: Catarina
authors.initials: C
authors.lastname: Franco
authors.forename: Fernando Capela
authors.initials: FC
authors.lastname: E Silva
authors.forename: Elvira Sales
authors.initials: ES
authors.lastname: Baptista
authors.forename: Ana Varela
authors.initials: AV
authors.lastname: Coelho