title: [Effects of Cu2+ on growth hormone secretion of pig pituitary cells in culture].
abstract: OBJECTIVE
  To study the effects of Cu2+ on growth hormone (GH) secretion of pig pituitary cells in vivo.
  
  
  METHODS
  Gland pituitary cells of 32-35 days old pigs were incubated for 48 h in presence of 10% fetal calf serum in DMEM. Cells were treated for 36 h with various concentrations of Cu2+ (0 mg/L, 0.025 mg/L, 0.1 mg/L, 0.4 mg/L, 1.6 mg/L) in serum-free DMEM. Culture medium was collected in 12 h, 24 h, 36 h thereafter for GH measurement and the radioimmunoassay kits were provided by Linco.
  
  
  RESULTS
  The GH concentration was higher in treatments of 0.025 mg/L, 0.1 mg/L, 0.4 mg/L than that of 0 mg/L when the cells were treated with Cu2+ for 24 h, and it was significantly different (P < 0.05) between treatment 0.1 mg/L and treatment 0 mg/L.
  
  
  CONCLUSIONS
  It was concluded that the presence of Cu2+ stimulated pig pituitary cells to secret GH in vivo.
authors.affiliation: College of Animal Science, South China Agriculture University, Guangzhou 510642, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Yu
authors.forename: Wei-Long
authors.initials: WL
authors.lastname: Fu
authors.forename: Ping-Xiang
authors.initials: PX
authors.lastname: Liu
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003300:Copper / Q000494:pharmacology*; D003470:Culture Media; D013006:Growth Hormone / Q000378:metabolism*; D010902:Pituitary Gland / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D013552:Swine
chemical_list: D003470:Culture Media; D003300:Copper; D013006:Growth Hormone