title: [Comparison between conventional methods, ChromAgar Candida® and PCR method for the identification of Candida species in clinical isolates].
abstract: The increase in the incidence of yeast species causing fungemia in susceptible immunocompromised patients in the last two decades and the low sensitivity of conventional blood culture has led to the need to develop alternative approaches for the early detection and identification of causative species. The aim of this study was to compare the usefulness of molecular testing by the polymerase chain reaction (PCR) and conventional methods to identify clinical isolates of different species, using the ID32C ATB system (bioMérieux, France), chromogenic culture Chromagar Candida® (CHROMagar, France) and morphogenesis in corn meal agar. We studied 79 isolates, in which the most prevalent species using the system ID32C and PCR was C. albicans, followed by C. tropicalis, C. glabrata and C .krusei. PCR patterns obtained for the identification of clinical isolates were stable and consistent in the various independent studies and showed good reproducibility, concluding that PCR with species-specific primers that amplify genes ITS1 and ITS2 for rRNA or topoisomerase II primers is a very specific and sensitive method for the identification of C. glabrata, C. krusei, C. albicans, and with less specificity for C. tropicalis.
authors.affiliation: Instituto de Ciencias Biomédicas, Universidad Autónoma de Ciudad Juárez, Ciudad Juárez, Chihuahua, México.
authors.forename: Deyanira
authors.initials: D
authors.lastname: Estrada-Barraza
authors.forename: Arturo
authors.initials: A
authors.lastname: Dávalos Martínez
authors.forename: Luis
authors.initials: L
authors.lastname: Flores-Padilla
authors.forename: Roberto
authors.initials: R
authors.lastname: Mendoza-De Elias
authors.forename: Luis Octavio
authors.initials: LO
authors.lastname: Sánchez-Vargas
mesh_terms: D000362:Agar; D002175:Candida / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification* / Q000472:pathogenicity; D002177:Candidiasis / Q000382:microbiology*; D002863:Chromogenic Compounds; D003470:Culture Media; D004250:DNA Topoisomerases, Type II / Q000235:genetics; D004271:DNA, Fungal / Q000302:isolation & purification; D005656:Fungal Proteins / Q000235:genetics; D016469:Fungemia / Q000382:microbiology*; D005800:Genes, Fungal; D006801:Humans; D009024:Morphogenesis; D009172:Mycology / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013045:Species Specificity; D014774:Virulence / Q000235:genetics
chemical_list: D002863:Chromogenic Compounds; D003470:Culture Media; D004271:DNA, Fungal; D005656:Fungal Proteins; D000362:Agar; D004250:DNA Topoisomerases, Type II