title: [Study on the feasibility and related factors on semen samples from condoms, collected from sexual partners of and by the female sex workers for HIV-antibody testing].
abstract: OBJECTIVE
  To explore the feasibility and related factors of using semen samples from condoms collected by their female sex workers (FSWs) for HIV-antibody testing.
  
  
  METHODS
  FSWs were recruited by outreach workers. Semen samples from condoms of their sexual partners (paid or regular) were collected by FSWs themselves after intercourse and for HIV testing. Male partners were asked to participate in the study. Questionnaires were administered for both FSWs and heir male sexual partners. Blood samples were also collected for HIV testing.
  
  
  RESULTS
  In total, 54 FSWs with 43 of their regular sexual partners and 57 casual clients were recruited. HIV prevalence, determined from serum samples, were 33.33% among FSWs, 29.82% and 23.26% among their clients and regular sexual partners. 40.35% and 30.23% of the semen samples from the condoms they used, were tested positive for HIV among clients and regular sexual partners of the FSWs. The sensitivity of semen samples from condoms for HIV-antibodies was 100% among both clients and regular sexual partners of the FSWs, while the rates of specificity were 85.00% and 90.91% respectively. Data from Univariate analysis indicated that among FSWs, factors as the characteristics of self-reported needle sharing and the male sexual partners who had one child, were associated with the disparity between serum and condom semen sample for HIV-antibody testing.
  
  
  CONCLUSIONS
  HIV prevalence in male clients and regular sexual partners of the FSWs might be overestimated according to the HIV-antibody testing results of semen samples from condoms collected by FSWs themselves. Lower specificity indicated that FSWs with positive HIV might have contaminated the semen samples from the condom used by their HIV negative sexual partners.
authors.affiliation: National Center for AIDS/STD Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
authors.forename: Heng
authors.initials: H
authors.lastname: Zhang
authors.forename: Ning
authors.initials: N
authors.lastname: Wang
authors.forename: Jun
authors.initials: J
authors.lastname: Bai
authors.forename: Gui-xiang
authors.initials: GX
authors.lastname: Wang
authors.forename: Dong-fang
authors.initials: DF
authors.lastname: Chang
authors.forename: Yun-sheng
authors.initials: YS
authors.lastname: Hou
authors.forename: Guo-wei
authors.initials: GW
authors.lastname: Ding
authors.forename: Xia
authors.initials: X
authors.lastname: Jin
authors.forename: Hai-bo
authors.initials: HB
authors.lastname: Wang
authors.forename: Chun-peng
authors.initials: CP
authors.lastname: Zang
authors.forename: Jun-jie
authors.initials: JJ
authors.lastname: Wang
mesh_terms: D000293:Adolescent; D000328:Adult; D017280:Condoms; D005240:Feasibility Studies; D005260:Female; D006678:HIV / Q000302:isolation & purification; D015483:HIV Antibodies / Q000032:analysis*; D015658:HIV Infections / Q000453:epidemiology*; D006801:Humans; D008297:Male; D012661:Semen / Q000821:virology*; D060386:Sex Workers; D012747:Sexual Partners; D055815:Young Adult
chemical_list: D015483:HIV Antibodies