title: Execution of the meiotic noncoding RNA expression program and the onset of gametogenesis in yeast require the conserved exosome subunit Rrp6.
abstract: Budding yeast noncoding RNAs (ncRNAs) are pervasively transcribed during mitosis, and some regulate mitotic protein-coding genes. However, little is known about ncRNA expression during meiotic development. Using high-resolution profiling we identified an extensive meiotic ncRNA expression program interlaced with the protein-coding transcriptome via sense/antisense transcript pairs, bidirectional promoters, and ncRNAs that overlap the regulatory regions of genes. Meiotic unannotated transcripts (MUTs) are mitotic targets of the conserved exosome component Rrp6, which itself is degraded after the onset of meiosis when MUTs and other ncRNAs accumulate in successive waves. Diploid cells lacking Rrp6 fail to initiate premeiotic DNA replication normally and cannot undergo efficient meiotic development. The present study demonstrates a unique function for budding yeast Rrp6 in degrading distinct classes of meiotically induced ncRNAs during vegetative growth and the onset of meiosis and thus points to a critical role of differential ncRNA expression in the execution of a conserved developmental program.
authors.affiliation: Institut National de Santé et de Recherche Médicale, Unité 625, Institut Fédératif de Recherche 140, Université de Rennes 1, F-35042 Rennes, France.
authors.forename: Aurélie
authors.initials: A
authors.lastname: Lardenois
authors.forename: Yuchen
authors.initials: Y
authors.lastname: Liu
authors.forename: Thomas
authors.initials: T
authors.lastname: Walther
authors.forename: Frédéric
authors.initials: F
authors.lastname: Chalmel
authors.forename: Bertrand
authors.initials: B
authors.lastname: Evrard
authors.forename: Marina
authors.initials: M
authors.lastname: Granovskaia
authors.forename: Angela
authors.initials: A
authors.lastname: Chu
authors.forename: Ronald W
authors.initials: RW
authors.lastname: Davis
authors.forename: Lars M
authors.initials: LM
authors.lastname: Steinmetz
authors.forename: Michael
authors.initials: M
authors.lastname: Primig
mesh_terms: D044127:Epigenesis, Genetic / Q000235:genetics / Q000502:physiology*; D005095:Exoribonucleases / Q000235:genetics / Q000378:metabolism*; D063326:Exosome Multienzyme Ribonuclease Complex; D005434:Flow Cytometry; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal / Q000235:genetics*; D008540:Meiosis / Q000502:physiology*; D008954:Models, Biological; D009841:Oligonucleotides / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics*; D022661:RNA, Untranslated / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D009841:Oligonucleotides; D012333:RNA, Messenger; D022661:RNA, Untranslated; D029701:Saccharomyces cerevisiae Proteins; D005095:Exoribonucleases; D063326:Exosome Multienzyme Ribonuclease Complex; C112610:RRP6 protein, S cerevisiae