title: The microdistribution of the trichomycete Smittium culisetae in the hindgut of the black fly host Simulium vittatum.
abstract: We examined the distribution of hyphae of the trichomycete fungus Smittium culisetae (Harpellales: Legeriomycetaceae) in the hindgut of a larval black fly (Simulium vittatum, cytospecies IS-7) by analyzing its prevalence and relative abundance. Hyphal prevalence was highest in the posterior colon (93.1%) and rectum (86.3%), with low prevalence (12.0%) in the anterior colon. Relative abundance of hyphae was highest in the posterior colon, followed by the rectum; relative abundance of hyphae in the anterior colon was lower. Hyphae of S. culisetae were not observed in the pylorus. We used a novel method of quantifying the relative abundance of S. culisetae in the host hindgut. The hindgut was observed with an ocular grid, and abundance was expressed as the ratio of grids occupied by hyphae to the number of grids occupied by hindgut.
authors.affiliation: Department of Biological Sciences, Life Sciences Building, Room 124, University of South Alabama, 307 University Blvd., Mobile, Alabama 36688-0002.
authors.forename: John W
authors.initials: JW
authors.lastname: McCreadie
authors.forename: Charles E
authors.initials: CE
authors.lastname: Beard