title: Expression and distribution of dopamine transporter in cardiac tissues of the guinea pig.
abstract: Dopamine transporter (DAT) is a membrane protein that it is a marker for dopaminergic neurons. In the present work, throught Western blot and autoradiographic studies with a selective ligand for DAT ([(3)H] WIN-35428) and noradrenaline transporter (NET) ([(3)H] Nisoxetine), we search the expression and distribution of DAT in comparison with NET, in cardiac tissue of guinea pig in order to support the presence of dopaminergic nerve cells into the heart. Expression of DAT, and NET were evidenced by a bands of 75 and 54 kDa, respectively in the heart. Binding for DAT and NET were found in the four cardiac chambers. However, DAT show heterogeneous distribution with binding in right atria and in both ventricles, whereas NET show homogenous distribution in the four cardiac chambers. The results show the expression of DAT in cardiac tissues with a different distribution compared with NET, being an evidence for the presence of dopaminergic nerve cells into the heart.
authors.affiliation: Instituto de Fisiología, Universidad Autonoma de Puebla, 14 Sur 6301, San Manuel, CP 72570, Puebla, Puebla, Mexico.
authors.forename: Alejandro Reynoso
authors.initials: AR
authors.lastname: Palomar
authors.forename: Berenice Navarrete
authors.initials: BN
authors.lastname: Larios
authors.forename: Victoria Chagoya
authors.initials: VC
authors.lastname: De Sánchez
authors.forename: Lidia Martínez
authors.initials: LM
authors.lastname: Pérez
authors.forename: Fidel De La Cruz
authors.initials: Fde L
authors.lastname: López
authors.forename: Gonzalo
authors.initials: G
authors.lastname: Flores
authors.forename: Maria de Jesús
authors.initials: Mde J
authors.lastname: Gómez-Villalobos
mesh_terms: D000818:Animals; D001345:Autoradiography; D050483:Dopamine Plasma Membrane Transport Proteins / Q000378:metabolism*; D006168:Guinea Pigs; D006321:Heart / Q000033:anatomy & histology*; D008297:Male; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D050484:Norepinephrine Plasma Membrane Transport Proteins / Q000378:metabolism
chemical_list: D050483:Dopamine Plasma Membrane Transport Proteins; D050484:Norepinephrine Plasma Membrane Transport Proteins