title: [Whole-cell recording of potassium and sodium currents in cortical, hippocampal, and sympathetic ganglia neurons].
abstract: OBJECTIVE
  To establish the culturing of cortical, hippocampal and sympathetic ganglia neurons derived from neonatal rats and the whole-cell recording techniques of potassium and sodium currents in cultured neurons.
  
  
  METHODS
  Cortex, hippocampus and sympathetic ganglion were isolated from neonatal rats (1-3 days) and digested by trypsin (0.125%). The neurons were cultured in polylysine covered petri dish (O35 mm) with DEME medium(high glucose) for seven days for electrophysiological experiments.
  
  
  RESULTS
  The neurons cultured by this method were in good conditions and could be sealed easily in whole-cell recording. I(Na), I(A), and I(K) could be recorded respectively in cultured neurons by different recording paradigms in Ca2+ -free extracellular solution.
  
  
  CONCLUSIONS
  It is a practicable and economical method for electrophysiological study of nervous system.
authors.affiliation: Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Wen-yu
authors.initials: WY
authors.lastname: Cui
authors.forename: Yin-li
authors.initials: YL
authors.lastname: Zhang
authors.forename: Xiao-feng
authors.initials: XF
authors.lastname: Yin
authors.forename: Hai
authors.initials: H
authors.lastname: Wang
mesh_terms: D000818:Animals; D015220:Calcium Channels / Q000502:physiology; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000502:physiology*; D005728:Ganglia, Sympathetic / Q000166:cytology / Q000502:physiology*; D006624:Hippocampus / Q000502:physiology*; D008564:Membrane Potentials / Q000502:physiology; D018408:Patch-Clamp Techniques; D015221:Potassium Channels / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D015222:Sodium Channels / Q000502:physiology
chemical_list: D015220:Calcium Channels; D015221:Potassium Channels; D015222:Sodium Channels