title: Expressional changes of RyR1 and RyR2 in PC12 cells after induction of apoptosis.
abstract: Ca2+ released from endoplasmic reticulum through ryanodine receptors (RyRs) and inositol 1,4,5-trisphosphate receptors (IP3Rs) can trigger apoptotic or necrotic pathways in cooperation with proapoptotic and/or prosurvival proteins, as those of Bcl-2 family. In such regulatory pathways expressional modulation of these Ca2+ transporters could also be expected. Therefore, our aim was to determine the expressional changes of RyR1 and RyR2 after experimental induction of apoptosis in PC12 cells. Our results showed significant decrease of RyR1 and RyR2 expressions, while caspase-3 and Bax expression significantly increased. We conclude that induction of apoptosis in PC12 cells could result in RyR expression down regulation.
authors.affiliation: Institute of Molecular Physiology and Genetics, Centre of Excellence for Cardiovascular Research, Slovak Academy of Sciences, Bratislava, Slovakia. dana.jurkovicova@savba.sk
authors.forename: Dana
authors.initials: D
authors.lastname: Jurkovicova
authors.forename: Lubomira
authors.initials: L
authors.lastname: Lencesova
authors.forename: Olga
authors.initials: O
authors.lastname: Krizanova
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000235:genetics; D053148:Caspase 3 / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects*; D016716:PC12 Cells; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D019837:Ryanodine Receptor Calcium Release Channel / Q000235:genetics*; D051028:bcl-2-Associated X Protein / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D019837:Ryanodine Receptor Calcium Release Channel; D051028:bcl-2-Associated X Protein; D053148:Caspase 3