title: A field effect transistor (FET)-based immunosensor for detection of HbA1c and Hb.
abstract: A field effect transistor (FET)-based immunosensor was developed for diabetes monitoring by detecting the concentrations of glycated hemoglobin (HbA1c) and hemoglobin (Hb). This immunosensor consists of a FET-based sensor chip and a disposable extended-gate electrode chip. The sensor chip was fabricated by standard CMOS process and was integrated with signal readout circuit. The disposable electrode chip, fabricated on polyester plastic board by Micro-Electro-Mechanical-Systems (MEMS) technique, was integrated with electrodes array and micro reaction pool. Biomolecules were immobilized on the electrode based on self-assembled monolayer and gold nanoparticles. Experimental results showed that the immunosensor achieved a linear response to HbA1c with the concentration from 4 to 24 μg/ml, and a linear response to Hb with the concentration from 60 to 180 μg/ml.
authors.affiliation: State Key Lab of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing, China. cbian@mail.ie.ac.cn
authors.forename: Chao
authors.initials: C
authors.lastname: Bian
authors.forename: Jianhua
authors.initials: J
authors.lastname: Tong
authors.forename: Jizhou
authors.initials: J
authors.lastname: Sun
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.forename: Qiannan
authors.initials: Q
authors.lastname: Xue
authors.forename: Shanhong
authors.initials: S
authors.lastname: Xia
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D015374:Biosensing Techniques / Q000295:instrumentation*; D002417:Cattle; D058266:Dielectric Spectroscopy; D004209:Disposable Equipment; D004563:Electrochemistry; D004566:Electrodes; D006442:Glycated Hemoglobin / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D006046:Gold / Q000737:chemistry; D055767:Immobilized Proteins / Q000737:chemistry; D007118:Immunoassay / Q000295:instrumentation*; D010984:Platinum / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry; D014173:Transistors, Electronic
chemical_list: D000906:Antibodies; D006442:Glycated Hemoglobin; D006046:Gold; D055767:Immobilized Proteins; D010984:Platinum; D012710:Serum Albumin, Bovine