title: A simple and reliable stir bar sorptive extraction-liquid chromatography procedure for the determination of chlorpromazine and trifluoperazine in human serum using experimental design methodology.
abstract: A sensitive and reproducible stir bar sorptive extraction and HPLC-UV detection method was used for the therapeutic drug monitoring of chlorpromazine and trifluoperazine in human serum. The separation was achieved using a C(18) column. The mobile phase consisted of methanol/sodium acetate buffer (pH 4.1; 0.1 M) (95:5, v/v) including 0.5% triethylamine. This miniaturized method can result in faster analysis, lower solvent consumption and less workload per sample while maintaining or even improving sensitivity. In the second part, stir bar sorptive extraction/HPLC-UV method was optimized by a chemometrics approach. An experimental design was therefore used to evaluate the statistically influential and/or interacting factors, among those described in the literature, and to find the best extraction and desorption conditions. Optimal sample volume of 1 mL, extraction time of 24 min at 31°C with pH 8.1 were obtained in a screening 2(5) half fractional factorial design followed by a Box-Behnken design. For the desorption conditions, a Box-Behnken design showed that the best conditions were 150 μL mobile phase for 20 min at 50°C. The optimized method was repeatable (CV<10%, linear (LOQ-500 ng/mL)), with the LOQs equal to 0.7 and 1.5 ng/mL for chlorpromazine and trifluoperazine, respectively.
authors.affiliation: Department of Chemistry, Faculty of Science, Alzahra University, Tehran, Iran.
authors.forename: Shaghayegh
authors.initials: S
authors.lastname: Bazhdanzadeh
authors.forename: Zahra
authors.initials: Z
authors.lastname: Talebpour
authors.forename: Noushin
authors.initials: N
authors.lastname: Adib
authors.forename: Hassan Y
authors.initials: HY
authors.lastname: Aboul-Enein
mesh_terms: D014150:Antipsychotic Agents / Q000097:blood*; D002746:Chlorpromazine / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods* / Q000592:standards; D016903:Drug Monitoring / Q000379:methods*; D006801:Humans; D015394:Molecular Structure; D015203:Reproducibility of Results; D012107:Research Design; D014268:Trifluoperazine / Q000097:blood*
chemical_list: D014150:Antipsychotic Agents; D014268:Trifluoperazine; D002746:Chlorpromazine