title: 6p21 rearrangements in uterine leiomyomas targeting HMGA1.
abstract: To quantify the expression of HMGA1 mRNA in uterine leiomyomas, the expression of HMGA1 was analyzed in a series including tumors with aberrations of chromosome 6 (n = 7) and cytogenetically normal tumors (n = 8) as a control group by quantitative reverse transcriptase-polymerase chain reaction. The average expression level in the 6p21 group was found to be 5.6 times higher than that in the control group, and with one exception, all cases with 6p21 alteration revealed a high expression of HMGA1 mRNA than cytogenetically normal tumors. Nevertheless, compared to fibroids with a normal karyotype, the upregulation of the HMGA1 mRNA in these cases was much less strong than that of HMGA2 mRNA in case of 12q14∼15 aberrations identified in previous studies.
authors.affiliation: Center for Human Genetics, University of Bremen, Leobener Str. ZHG, D-28359 Bremen, Germany.
authors.forename: Maliheh Hashemi
authors.initials: MH
authors.lastname: Nezhad
authors.forename: Norbert
authors.initials: N
authors.lastname: Drieschner
authors.forename: Sabrina
authors.initials: S
authors.lastname: Helms
authors.forename: Anke
authors.initials: A
authors.lastname: Meyer
authors.forename: Mahboobeh
authors.initials: M
authors.lastname: Tadayyon
authors.forename: Markus
authors.initials: M
authors.lastname: Klemke
authors.forename: Gazanfer
authors.initials: G
authors.lastname: Belge
authors.forename: Sabine
authors.initials: S
authors.lastname: Bartnitzke
authors.forename: Käte
authors.initials: K
authors.lastname: Burchardt
authors.forename: Christiane
authors.initials: C
authors.lastname: Frantzen
authors.forename: Ernst Heinrich
authors.initials: EH
authors.lastname: Schmidt
authors.forename: Jörn
authors.initials: J
authors.lastname: Bullerdiek
mesh_terms: D002869:Chromosome Aberrations; D002871:Chromosome Banding; D002896:Chromosomes, Human, Pair 6; D003582:Cytogenetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D015321:Gene Rearrangement; D025741:HMGA1a Protein / Q000235:genetics*; D025743:HMGA2 Protein / Q000235:genetics; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D007889:Leiomyoma / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism; D014594:Uterine Neoplasms / Q000235:genetics*
chemical_list: D025743:HMGA2 Protein; D012333:RNA, Messenger; D025741:HMGA1a Protein