title: [Screening of target protein interacting with lipid transfer protein AsE246 of Astragalus sinicus and expression characteristics of corresponding encoding gene].
abstract: OBJECTIVE
  AsE246 was a novel nodule-specific expressed nodulin gene encoding non-specific lipid transfer protein 1 (nsLTP1) of A. sinicus. We screened and identified host plant target protein interacting with AsE246, and characterized the expression patterns of target gene under symbiotic and stress conditions.
  
  
  METHODS
  Yeast two-hybrid system, small-scale yeast hybridization test and real-time PCR technique were used to capture the host target protein that interacts with the bait protein AsE246, and to quantitatively analyze the temporal and spatial expression characteristics of target gene during root nodule development and nitrogen fixation process.
  
  
  RESULTS
  One positive clone was obtained, its cDNA insert sequence and Blast analysis showed that: the target protein was a DnaJ-like protein, thus the corresponding encoding gene was named as AsDJL1. AsDJL1 was specifically-enhancing expressed in nitrogen-fixing root nodules, and significantly increased under NaCl stress while significantly decreased under (NH4) 2SO4 stress.
  
  
  CONCLUSIONS
  This work was the first report on the isolation of proteins interacting with LTP. The work obtained some direct and convincing evidences to show the interacting gene demonstrated high similarity as AsE246 in expression patterns and functions involved. The present progress provided a basic foundation and theoretical basis to undertake any further investigation into their interaction, and regulation mechanism associated with symbiotic nitrogen fixation or response to environmental stress.
authors.affiliation: State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China. qingyan0812@163.com
authors.forename: Qingyan
authors.initials: Q
authors.lastname: Zhu
authors.forename: Yixing
authors.initials: Y
authors.lastname: Li
authors.forename: Ning
authors.initials: N
authors.lastname: Wang
authors.forename: Dasong
authors.initials: D
authors.lastname: Chen
authors.forename: Youguo
authors.initials: Y
authors.lastname: Li
mesh_terms: D000595:Amino Acid Sequence; D027883:Astragalus Plant / Q000235:genetics* / Q000378:metabolism; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D018076:DNA, Complementary / Q000032:analysis; D004353:Drug Evaluation, Preclinical / Q000379:methods; D004347:Drug Interactions; D018506:Gene Expression Regulation, Plant; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009586:Nitrogen Fixation / Q000235:genetics; D009693:Nucleic Acid Hybridization; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D013559:Symbiosis
chemical_list: D002352:Carrier Proteins; D018076:DNA, Complementary; D008565:Membrane Proteins; D010940:Plant Proteins; C030363:lipid transfer protein; C045625:nodulin; C072087:sterol carrier proteins