title: Antimicrobial organophilic montmorillonite nanoparticles: screening and detection assay.
abstract: The present study presents the development of a standard protocol for detection and screening of nanoparticle(s) for their antimicrobial activity with particular reference to organophilic montmorillonite (Ommt). For this purpose, Ommt nanoparticles have been synthesized through cation exchange of commercial montmorillonite (K10) with a cetyl pyridinium bromide. The formation of Ommt has been ascertained through UV-visible, Fourier transform infrared spectroscopy, X-ray diffraction spectra, and transmission electron microscopy. Subsequently, "zone of inhibition" and "bacterial killing" assays were performed by incubating the four Gram-negative test bacteria with Ommt, to determine antimicrobial activity and reduction in colony forming unit per mL (confirmative test), respectively. The developed assay will provide an easy approach over conventional disc diffusion antibiotic sensitivity test, to study the impact of different nanoparticles against different bacterial species.
authors.affiliation: Department of Microbiology, GB Pant University of Agriculture & Technology, Pantnagar, Uttarakhand, India.
authors.forename: Harshita
authors.initials: H
authors.lastname: Negi
authors.forename: Tithi
authors.initials: T
authors.lastname: Agarwal
authors.forename: Mohd Ghulan Haider
authors.initials: MG
authors.lastname: Zaidi
authors.forename: Anil
authors.initials: A
authors.lastname: Kapri
authors.forename: Reeta
authors.initials: R
authors.lastname: Goel
mesh_terms: D001546:Bentonite / Q000737:chemistry* / Q000494:pharmacology*; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000648:ultrastructure; D017550:Spectroscopy, Fourier Transform Infrared; D014961:X-Ray Diffraction
chemical_list: D001546:Bentonite