title: Real-time cancellation of temperature induced resonance shifts in SOI wire waveguide ring resonator label-free biosensor arrays.
abstract: A comprehensive investigation of real-time temperature-induced resonance shift cancellation for silicon wire based biosensor arrays is reported for the first time. A reference resonator, protected by either a SU8 or SiO(2) cladding layer, is used to track temperature changes. The temperature dependence of resonators in aqueous solutions, pertinent to biosensing applications, is measured under steady-state conditions and the operating parameters influencing these properties are discussed. Real-time measurements show that the reference resonator resonances reflect the temperature changes without noticeable time delay, enabling effective cancellation of temperature-induced shifts. Binding between complementary IgG protein pairs is monitored over 4 orders of magnitude dynamic range down to a concentration of 20 pM, demonstrating a resolvable mass of 40 attograms. Reactions are measured over time periods as long as 3 hours with high stability, showing a scatter corresponding to a fluid refractive index fluctuation of ± 4 × 10(-6) in the baseline data. Sensor arrays with a SU8 protective cladding are easy to fabricate, while oxide cladding is found to provide superior stability for measurements involving long time scales.
authors.affiliation: Institute for Microstructural Sciences, National Research Council Canada, 1200 Montreal Rd, Ottawa, Ontario K1A 0R6, Canada. Danxia.Xu@nrc-cnrc.gc.ca
authors.forename: D-X
authors.initials: DX
authors.lastname: Xu
authors.forename: M
authors.initials: M
authors.lastname: Vachon
authors.forename: A
authors.initials: A
authors.lastname: Densmore
authors.forename: R
authors.initials: R
authors.lastname: Ma
authors.forename: S
authors.initials: S
authors.lastname: Janz
authors.forename: A
authors.initials: A
authors.lastname: Delâge
authors.forename: J
authors.initials: J
authors.lastname: Lapointe
authors.forename: P
authors.initials: P
authors.lastname: Cheben
authors.forename: J H
authors.initials: JH
authors.lastname: Schmid
authors.forename: E
authors.initials: E
authors.lastname: Post
authors.forename: Sonia
authors.initials: S
authors.lastname: Messaoudène
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Fédéli
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods*; D004560:Electricity; D007074:Immunoglobulin G / Q000032:analysis; D011817:Rabbits; D012825:Silicon / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry; D013057:Spectrum Analysis; D013194:Staining and Labeling; D013696:Temperature; D013997:Time Factors
chemical_list: D007074:Immunoglobulin G; D012822:Silicon Dioxide; D012825:Silicon