title: [The chronic effect of palmitic acid on apoptosis of pancreatic islet beta-cells and the mechanism].
abstract: OBJECTIVE
  To investigate the chronic effect of palmitic acid (PA) on apoptosis of pancreatic islet beta-cells and the possible mechanism.
  
  
  METHODS
  Insulinoma cell line (MIN6 cells) were used in this study. After being incubated in PA (0.1 - 1.6 mml/L) for 24 and 48 hours, MTT method was used to evaluate the livability. After being incubated for 48 h, Hoechst-PI and Annexin-V-FTTC/PI FACS were used to estimate the apoptosis in each group, Western-blotting assay was used to estimate the protein level of p-Akt, Akt, Bax and Bcl-2.
  
  
  RESULTS
  Chronic PA dose-dependently (1) decreased the availability and increased the apoptosis of MIN6 cells; (2) decreased the phosphorylation of Akt and Bcl-2, but had no significant effects on Akt and Bax.
  
  
  CONCLUSIONS
  Chronic PA dose-dependently induced apoptosis of MIN6 cells, and this effect was possibly regulated by Akt/Bcl-2.
authors.affiliation: Fujian Institute of Endocrinology, Department of Endocrinology, Union Hospital, Fujian Medical University, Fuzhou 350001, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Liu
authors.forename: Ran-Ping
authors.initials: RP
authors.lastname: Wang
authors.forename: Xiao-Hong
authors.initials: XH
authors.lastname: Liu
authors.forename: Lin-Xi
authors.initials: LX
authors.lastname: Wang
authors.forename: Xiao-Ying
authors.initials: XY
authors.lastname: Liu
authors.forename: Wen-Jia
authors.initials: WJ
authors.lastname: Chen
authors.forename: Li-Bin
authors.initials: LB
authors.lastname: Liu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D007340:Insulinoma / Q000473:pathology; D007515:Islets of Langerhans / Q000473:pathology*; D051379:Mice; D018384:Oxidative Stress / Q000502:physiology*; D019308:Palmitic Acid / Q000494:pharmacology*; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D019253:Proto-Oncogene Proteins c-bcl-2; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: C494827:Bax protein, mouse; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; C052617:Bcl2 protein, mouse; D019308:Palmitic Acid; D051057:Proto-Oncogene Proteins c-akt