title: Elizabethkingia anophelis sp. nov., isolated from the midgut of the mosquito Anopheles gambiae.
abstract: The taxonomic position, growth characteristics and antibiotic resistance properties of a slightly yellow-pigmented bacterial strain, designated R26(T), isolated from the midgut of the mosquito Anopheles gambiae, were studied. The isolate produced rod-shaped cells, which stained Gram-negative. The bacterium had two growth optima at 30-31 °C and 37 °C. Strain R26(T) demonstrated natural antibiotic resistance to ampicillin, chloramphenicol, kanamycin, streptomycin and tetracycline. 16S rRNA gene sequence analysis revealed that the isolate showed 98.6 % sequence similarity to that of Elizabethkingia meningoseptica ATCC 13253(T) and 98.2 % similarity to that of Elizabethkingia miricola GTC 862(T). The major fatty acids of strain R26(T) were iso-C(15 : 0), iso-C(17 : 0) 3-OH and summed feature 4 (iso-C(15 : 0) 2-OH and/or C(16 : 1)ω7c/t). Strain R26(T) contained only menaquinone MK-6 and showed a complex polar lipid profile consisting of diphosphatidylglycerol, phosphatidylinositol, an unknown phospholipid and unknown polar lipids and glycolipids. DNA-DNA hybridization experiments with E. meningoseptica CCUG 214(T) ( = ATCC 13253(T)) and E. miricola KCTC 12492(T) ( = GTC 862(T)) gave relatedness values of 34.5 % (reciprocal 41.5 %) and 35.0 % (reciprocal 25.7 %), respectively. DNA-DNA hybridization results and some differentiating biochemical properties indicate that strain R26(T) represents a novel species, for which the name Elizabethkingia anophelis sp. nov. is proposed. The type strain is R26(T) ( = CCUG 60038(T) = CCM 7804(T)).
authors.affiliation: Institut für Angewandte Mikrobiologie, Universität Giessen, Giessen, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Kämpfer
authors.affiliation: Department of Genetics, Microbiology and Toxicology, Stockholm University, S-106 91 Stockholm, Sweden.
authors.forename: Holly
authors.initials: H
authors.lastname: Matthews
authors.affiliation: Institut für Mikro- und Molekularbiologie, Universität Giessen, Giessen, Germany.
authors.forename: Stefanie P
authors.initials: SP
authors.lastname: Glaeser
authors.affiliation: Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie eV, Hans-Knöll-Institut, Jena, Germany.
authors.forename: Karin
authors.initials: K
authors.lastname: Martin
authors.affiliation: Institut für Angewandte Mikrobiologie, Universität Giessen, Giessen, Germany.
authors.forename: Nicole
authors.initials: N
authors.lastname: Lodders
authors.affiliation: Department of Genetics, Microbiology and Toxicology, Stockholm University, S-106 91 Stockholm, Sweden.
authors.forename: Ingrid
authors.initials: I
authors.lastname: Faye
mesh_terms: D000818:Animals; D000852:Anopheles / Q000382:microbiology*; D004275:DNA, Ribosomal / Q000235:genetics; D005227:Fatty Acids / Q000378:metabolism; D041965:Flavobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D041981:Gastrointestinal Tract / Q000382:microbiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S