title: [Role of nuclear factor-kappaB in apoptosis pathway of HUVEC].
abstract: OBJECTIVE
  To investigate the role of nuclear factor-kappaB in apoptosis pathway of HUVEC.
  
  
  METHODS
  The cell lines of HUVEC cultured in vitro were divided into three groups: normal control group, Ang II group, and Gliotoxin group. We investigated the effects of Ang II (0.01 micromol/L, 0.1 micromol/L, 1 micromol/L and 10 micromol/L) on the viability of HUVEC with modified MTT. Then agarose gel electrophoresis and flow cytometry were applied to detect the apoptosis of HUVEC. Finally, the nuclear translocation of NF-kappaB subunit p65 was evaluated by immunocytochemistry.
  
  
  RESULTS
  The viability of HUVEC decreased significantly after incubated with 10 micromol/L Ang II for 24 hours. The results of DNA agarose gel and flow cytometry showed that 10 micromol/L Ang II induced the apoptosis of HUVEC, and the apoptosis rate was significantlyhigher than normal control group (P < 0.05). 0.1 mg/L Gliotoxin antagonized this effect of Ang II. The results of immunocytochemistry suggested that NF-kappaB was activated in HUVEC induced by 10 micromol/L Ang II. In contrast, Gliotoxin inhibited the activation of NF-kappaB in HUVEC induced by Ang II.
  
  
  CONCLUSIONS
  (1) Ang II can induce the apoptosis of HUVEC, while the inhibitor of NF-kappaB, Gliotoxin, can antagonize the effect of Ang II. (2) NF-kappaB may play an important role in apoptosis pathway of HUVEC induced by Ang II.
authors.affiliation: Department of Pathology, The 1st Affliated Hospital of Kunming Medical University, Kunming 650032, China.
authors.forename: Feng-lin
authors.initials: FL
authors.lastname: Xue
authors.forename: Xuan
authors.initials: X
authors.lastname: Zhang
authors.forename: Guo-yang
authors.initials: GY
authors.lastname: He
authors.forename: Dian-hua
authors.initials: DH
authors.lastname: Wang
mesh_terms: D000804:Angiotensin II / Q000494:pharmacology*; D017209:Apoptosis / Q000502:physiology*; D002478:Cells, Cultured; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology*; D006801:Humans; D016328:NF-kappa B / Q000502:physiology*; D015398:Signal Transduction / Q000502:physiology
chemical_list: D016328:NF-kappa B; D000804:Angiotensin II