title: Zinc(II)-dipicolylamine-functionalized polydiacetylene-liposome microarray: a selective and sensitive sensing platform for pyrophosphate ions.
abstract: A microarray-chip assay system for the fluorescence detection of phosphate-containing analytes in aqueous media has been constructed from stimuli-responsive polymerized poly(diacetylene)-liposomes for the first time. Proper combination of the liposome components (Zn(II)-dipicolylamine for phosphate binding and an amine-terminated component for anchoring the liposome onto an aldehyde-derivatized glass plate), has led to a microarray chip that selectively detects pyrophosphate, an important biomarker, over competing anions, such as phosphate and adenosine triphosphate, with nanomolar sensitivity. The chip-based assay shows advantages, such as high specificity and sensitivity, over solution-based assays that use the same liposomes, and over known homogeneous molecular sensing systems.
authors.affiliation: Department of Chemistry and Center for Electro-Photo Behaviors in Advanced Molecular Systems, Pohang University of Science and Technology (POSTECH), San 31 Hyoja-dong, Pohang 790-784, Korea.
authors.forename: Kyung Mi
authors.initials: KM
authors.lastname: Kim
authors.forename: Dong Ju
authors.initials: DJ
authors.lastname: Oh
authors.forename: Kyo Han
authors.initials: KH
authors.lastname: Ahn
mesh_terms: D000588:Amines / Q000737:chemistry; D011756:Diphosphates / Q000737:chemistry*; D005453:Fluorescence; D008081:Liposomes / Q000737:chemistry; D046228:Microarray Analysis / Q000295:instrumentation* / Q000379:methods; D008855:Microscopy, Electron, Scanning; D008958:Models, Molecular; D010848:Picolinic Acids / Q000737:chemistry; D000078789:Polyacetylene Polymer; D011108:Polymers / Q000737:chemistry*; D053279:Polyynes / Q000737:chemistry*; D015032:Zinc / Q000737:chemistry*
chemical_list: C501847:2,2'-dipicolylamine; D000588:Amines; D011756:Diphosphates; D008081:Liposomes; D010848:Picolinic Acids; D000078789:Polyacetylene Polymer; D011108:Polymers; D053279:Polyynes; C082361:polydiacetylene; D015032:Zinc