title: Antitumor effect of endostatin overexpressed in C6 glioma cells is associated with the down-regulation of VEGF.
abstract: Endostatin is an anti-angiogenic agent that blocks endothelial cell proliferation, tumor growth, and metastasis. Several lines of direct evidence show that gliomas express high levels of endostatin. However, the anti-angiogenic activity and cellular mechanisms of endostatin from tumor cells have not been completely elucidated. In this study, we established C6 glioblastoma (GBM) xenografts in nude mice by subcutaneously injecting glioma cell lines, C6-null cells, and stable transfected-C6 cells overexpressing mock vector (C6-mock) and endostatin (C6-endo). We found that overexpression of endostatin in C6-endo cells significantly suppressed the expression of VEGF in tumor cells in vivo as well as in vitro. Furthermore, the tumor growth derived from C6-endo cells was inhibited. Our data demonstrate that endostatin could play a direct role in inhibiting tumor cells, and suggest that enhancing endostatin expression in gliomas could be an effective treatment strategy.
authors.affiliation: Department of Pharmacology, The First Affiliated Hospital, Fujian Medical University, Fuzhou, PR China.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Yang
authors.forename: Zhixiong
authors.initials: Z
authors.lastname: Lin
authors.forename: Jianhua
authors.initials: J
authors.lastname: Lin
authors.forename: Shenmei
authors.initials: S
authors.lastname: Weng
authors.forename: Qian
authors.initials: Q
authors.lastname: Huang
authors.forename: Pengfei
authors.initials: P
authors.lastname: Zhang
authors.forename: Jin
authors.initials: J
authors.lastname: Fu
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology; D020533:Angiogenesis Inhibitors; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D015153:Blotting, Western; D001932:Brain Neoplasms / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004195:Disease Models, Animal; D015536:Down-Regulation; D043169:Endostatins / Q000235:genetics*; D015870:Gene Expression; D005822:Genetic Vectors; D005909:Glioblastoma / Q000378:metabolism* / Q000473:pathology; D007124:Immunoenzyme Techniques; D008175:Lung Neoplasms / Q000378:metabolism / Q000556:secondary; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009943:Organophosphorus Compounds / Q000494:pharmacology*; D051381:Rats; D042442:Vascular Endothelial Growth Factors / Q000378:metabolism*
chemical_list: D020533:Angiogenesis Inhibitors; D043169:Endostatins; C455219:N-acetylcochinol-O-phosphate; D009943:Organophosphorus Compounds; D042442:Vascular Endothelial Growth Factors