title: [PI3K activity is associated with expression of neural specific genes in mouse fetal liver cells enhanced by butylated hydroxyanisole].
abstract: OBJECTIVE
  To study the mechanism of butylated hydroxyanisole-induced neural differentiation of fetal liver cells in vitro.
  
  
  METHODS
  14.5-day-old mouse fetal liver-derived cells were cultured, and were induced by 200 micromol/L butylated hydroxyanisole (BHA) combined with PI3K inhibitor LY294002 (20 micromol/L), and then were incubated in serum-free medium. Expression of genes in treated or untreated cells were assayed by Western blotting or RT-PCR.
  
  
  RESULTS
  There was low level of neurofilament-L (NF-L) and brain factor-1 (BF-1) but no neurofilament-H (NF-H) and tyrosine hydroxylase (TH) in fetal liver cells. BHA promoted significantly expression of neuron-specific NF-L, NF-H, BF-1, and TH in fetal liver cells. NF-L mRNA increased 5.8 fold, NF-H mRNA 8.0 fold, BF-1 mRNA 2.68 fold, and TH mRNA 30 fold, respectively (all P < 0.01 vs untreated cells). NF-L protein increased 11.29 fold, NF-H 5.5 fold, BF-1 2.53 fold, TH 4.76 fold. Moreover, expression of these BHA-induced genes were inhibited by PI3K inhibitor LY294002.
  
  
  CONCLUSIONS
  BHA induced neural differentiation of fetal liver cells through PI3K.
authors.affiliation: Institute of Hematology, Medical College of Jinan University, Guangzhou 510632, China.
authors.forename: Ge-Xiu
authors.initials: GX
authors.lastname: Liu
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Dong-Mei
authors.initials: DM
authors.lastname: He
mesh_terms: D000818:Animals; D002083:Butylated Hydroxyanisole / Q000494:pharmacology*; D002478:Cells, Cultured; D004622:Embryo, Mammalian / Q000166:cytology; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*
chemical_list: D009419:Nerve Tissue Proteins; D002083:Butylated Hydroxyanisole; D019869:Phosphatidylinositol 3-Kinases