title: Enhancement of single-molecule fluorescence signals by colloidal silver nanoparticles in studies of protein translation.
abstract: Metal-enhanced fluorescence (MEF) increased total photon emission of Cy3- and Cy5-labeled ribosomal initiation complexes near 50 nm silver particles 4- and 5.5-fold, respectively. Fluorescence intensity fluctuations above shot noise, at 0.1-5 Hz, were greater on silver particles. Overall signal-to-noise ratio was similar or slightly improved near the particles. Proximity to silver particles did not compromise ribosome function, as measured by codon-dependent binding of fluorescent tRNA, dynamics of fluorescence resonance energy transfer between adjacent tRNAs in the ribosome, and tRNA translocation induced by elongation factor G.
authors.affiliation: Department of Molecular Biology and Immunology, UNTHSC, Fort Worth, Texas 76107, United States.
authors.forename: Shashank
authors.initials: S
authors.lastname: Bharill
authors.forename: Chunlai
authors.initials: C
authors.lastname: Chen
authors.forename: Benjamin
authors.initials: B
authors.lastname: Stevens
authors.forename: Jaskiran
authors.initials: J
authors.lastname: Kaur
authors.forename: Zeev
authors.initials: Z
authors.lastname: Smilansky
authors.forename: Wlodek
authors.initials: W
authors.lastname: Mandecki
authors.forename: Ignacy
authors.initials: I
authors.lastname: Gryczynski
authors.forename: Zygmunt
authors.initials: Z
authors.lastname: Gryczynski
authors.forename: Barry S
authors.initials: BS
authors.lastname: Cooperman
authors.forename: Yale E
authors.initials: YE
authors.lastname: Goldman
mesh_terms: D001483:Base Sequence; D002232:Carbocyanines / Q000737:chemistry; D003102:Colloids; D031541:Fluorescence Resonance Energy Transfer; D053768:Metal Nanoparticles / Q000737:chemistry*; D038761:Photobleaching; D017785:Photons; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics; D012343:RNA, Transfer / Q000235:genetics / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism; D012834:Silver / Q000737:chemistry*
chemical_list: D002232:Carbocyanines; D003102:Colloids; D012333:RNA, Messenger; C089187:cyanine dye 3; C085321:cyanine dye 5; D012834:Silver; D012343:RNA, Transfer