title: Rapid, semiautomated quantification of bacterial cells in freshwater by using a microfluidic device for on-chip staining and counting.
abstract: A microfluidic device-based system for the rapid and semiautomated counting of bacteria in freshwater was fabricated and examined. Bacteria in groundwater and in potable water, as well as starved Escherichia coli O157:H7 spiked in pond water, were able to be on-chip stained and enumerated within 1 h using this system.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Nobuyasu
authors.initials: N
authors.lastname: Yamaguchi
authors.forename: Masashi
authors.initials: M
authors.lastname: Torii
authors.forename: Yuko
authors.initials: Y
authors.lastname: Uebayashi
authors.forename: Masao
authors.initials: M
authors.lastname: Nasu
mesh_terms: D058491:Bacterial Load / Q000295:instrumentation / Q000379:methods; D004129:Dimethylpolysiloxanes; D019453:Escherichia coli O157 / Q000302:isolation & purification; D005434:Flow Cytometry / Q000379:methods; D005618:Fresh Water / Q000382:microbiology*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods; D008856:Microscopy, Fluorescence
chemical_list: D004129:Dimethylpolysiloxanes; C013830:baysilon