title: Increase of Drosophila melanogaster lifespan due to D-GADD45 overexpression in the nervous system.
abstract: The GADD45 protein family plays an important role in stress signaling and participates in the integration of cellular response to environmental and physiological factors. GADD45 proteins are involved in cell cycle control, DNA repair, apoptosis, cell survival and aging, and inflammatory response by complicated protein-protein interactions. In Drosophila melanogaster a single D-GADD45 ortholog (GG1086) has been described. Our data show that overexpression of the D-GADD45 gene in the nervous system leads to a significantly increase of Drosophila lifespan without a decrease in fecundity and locomotor activity. The lifespan extension effect is more pronounced in males than in females, which agrees with the sex-dependent expression of this gene. The longevity of D. melanogaster with D-GADD45 overexpression is apparently due to more efficient recognition and repair of DNA damage, as the DNA comet assay showed that the spontaneous DNA damage in the larva neuroblasts is reduced with statistical significance.
authors.affiliation: Ural Division, Komi Science Center, Institute of Biology, Russian Academy of Sciences, Syktyvkar, Russia.
authors.forename: E N
authors.initials: EN
authors.lastname: Plyusnina
authors.forename: M V
authors.initials: MV
authors.lastname: Shaposhnikov
authors.forename: A A
authors.initials: AA
authors.lastname: Moskalev
mesh_terms: D000375:Aging / Q000235:genetics / Q000502:physiology; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D004331:Drosophila melanogaster / Q000502:physiology*; D005260:Female; D005298:Fertility; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics*; D008136:Longevity / Q000235:genetics*; D008297:Male; D009043:Motor Activity; D009420:Nervous System / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000097642:GADD45 Proteins
chemical_list: D017931:DNA Primers; D047908:Intracellular Signaling Peptides and Proteins; D000097642:GADD45 Proteins