title: Mutation in the beta adducin subunit causes tissue-specific damage to myogenic tone.
abstract: BACKGROUND
  Damage to renal artery myogenic tone is universally associated with progressive kidney damage. Recently, we have observed that mutations in the beta adducin subunit are associated with proteinuria in the Milan rat. Because of the role of adducin as a component of the cytoskeleton we hypothesized that this mutation may be associated with changes in myogenic tone.
  
  
  RESULTS
  Congenic rats were generated with beta adducin subunit mutation (NB rats) and compared with a previously studied rat model with alpha adducin subunit mutation (NAs rats). Blood pressure and urinary protein excretion were studied at two time points: 6 weeks and 4 months of age, and at these time points, small renal, middle cerebral and skeletal (cremaster) arteries were isolated and studied using pressure myography. Agonist-induced vasoconstriction was not different between the two groups at any age. However, myogenic tone in renal arteries was significantly damaged in the NB rat compared to its NAs counterpart and this was associated with a decrease in vascular distensibility. There was a smaller reduction in myogenic tone in the middle cerebral arteries from the NB rat, whereas in the skeletal arteries there was no difference between the two strains. In the NB rat, this tissue-specific damage to myogenic tone was associated with progressive proteinuria despite lower blood pressure than the NAs rat.
  
  
  CONCLUSIONS
  Mutations in the beta subunit of the adducin protein result in damage to renal artery myogenic tone and this is associated with renal damage as manifest by proteinuria.
authors.affiliation: Shimane Prefectural Central Hospital, Shimane, Japan.
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Sonoyama
authors.forename: Adam S
authors.initials: AS
authors.lastname: Greenstein
authors.forename: Rosella
authors.initials: R
authors.lastname: Micheletti
authors.forename: Patrizia
authors.initials: P
authors.lastname: Ferrari
authors.forename: Antonio
authors.initials: A
authors.lastname: Schiavone
authors.forename: Reza
authors.initials: R
authors.lastname: Aghamohammadzadeh
authors.forename: Sarah B
authors.initials: SB
authors.lastname: Withers
authors.forename: Grazia
authors.initials: G
authors.lastname: Tripodi
authors.forename: Mara
authors.initials: M
authors.lastname: Ferrandi
authors.forename: Anthony M
authors.initials: AM
authors.lastname: Heagerty
mesh_terms: D000367:Age Factors; D000818:Animals; D001794:Blood Pressure; D002148:Calmodulin-Binding Proteins / Q000235:genetics / Q000502:physiology*; D042783:Endothelial Cells / Q000502:physiology; D009154:Mutation; D009928:Organ Specificity; D010656:Phenylephrine / Q000494:pharmacology; D011507:Proteinuria / Q000517:prevention & control; D051381:Rats; D012077:Renal Artery / Q000502:physiology*; D014661:Vasoconstriction / Q000187:drug effects; D000099291:Adducins
chemical_list: D002148:Calmodulin-Binding Proteins; D010656:Phenylephrine; D000099291:Adducins