title: Primary squamous cell carcinoma of the vagina: human papillomavirus detection, p16(INK4A) overexpression and clinicopathological correlations.
abstract: OBJECTIVE
  To determine the role of human papillomavirus (HPV) in the pathogenesis of primary squamous cell carcinoma of the vagina (SCCVa), and to evaluate its clinicopathological significance.
  
  
  RESULTS
  All cases of SCCVa diagnosed over a 15-year period from two hospitals in Barcelona, Spain (n=32) were retrieved. Patients with a history of carcinoma of the cervix diagnosed <5 years before were excluded. HPV was detected and typed by polymerase chain reaction (PCR) using SPF10 primers. Immunohistochemistry was performed for p16 and p53. HPV was detected in 25 cases (78.1%). HPV16 was the most prevalent type. Patients with HPV-positive tumours were associated frequently with a history of carcinoma or intraepithelial neoplasia of the cervix or vulva diagnosed more than 5 years before (56% versus 0%; P=0.01). HPV-positive tumours were more frequently of non-keratinizing, basaloid or warty type than HPV-negative neoplasms (84% versus 14.3%; P<0.001), and showed diffuse positive immunoreactivity for p16(INK4a) (96%, versus 14.3%; P<0.001). The sensitivity and specificity of p16 to identify HPV-positive tumours were 96% and 85.7%, respectively.
  
  
  CONCLUSIONS
  A high number of SCCVs are related to HPV infection and may be identified by immunohistochemistry for p16. HPV-positive tumours tend to affect women with history of cervical neoplasia.
authors.affiliation: Department of Pathology, Hospital Clinic-IDIBAPS, University of Barcelona, Barcelona, Spain.
authors.forename: Victoria
authors.initials: V
authors.lastname: Fuste
authors.forename: Marta
authors.initials: M
authors.lastname: del Pino
authors.forename: Assumpta
authors.initials: A
authors.lastname: Perez
authors.forename: Angel
authors.initials: A
authors.lastname: Garcia
authors.forename: Aureli
authors.initials: A
authors.lastname: Torne
authors.forename: Jaume
authors.initials: J
authors.lastname: Pahisa
authors.forename: Jaume
authors.initials: J
authors.lastname: Ordi
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000473:pathology* / Q000821:virology; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D027383:Papillomaviridae / Q000235:genetics*; D030361:Papillomavirus Infections / Q000378:metabolism / Q000473:pathology* / Q000821:virology; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D014625:Vaginal Neoplasms / Q000378:metabolism / Q000473:pathology* / Q000821:virology
chemical_list: D019941:Cyclin-Dependent Kinase Inhibitor p16