title: [Vacuole formation and endothelial damage in microvessels after ischemia reperfusion].
abstract: OBJECTIVE
  Damage of endothelial cells and adhesion of leukocytes and platelets were studied in rat mesentery microvessels after ischemia reperfusion.
  
  
  METHODS
  The model was made by losing blood and reperfusion from carotid artery in rat. Changes of mesentery microvessels were observed by high magnify microscope.
  
  
  RESULTS
  Leukocytes and platelets adhesions were found in venules and co capillaries 1-3 hours after ischemia reperfusion. Endothelial cells were edema and vascular walls were thickening. Vacuoles formed in intracytoplasm of some vascular endothelium and some bigger endothelial vacuoles prominence toward the luminal surface. Vacuoles liked circle shape and the diameter was 10-30 microm. More vacuoles were found in arterioles, even there were several vacuoles in a arteriole. The biggest vacuole almost occupied 2/3 of vascular lumen.
  
  
  CONCLUSIONS
  Edema and vacuole formation in vascular endothelium indicate that endothelial cells are injured seriously after ischemia reperfusion.
authors.affiliation: Laboratory of Microcirculaiton PLA General Hospital, Beijing 100853.
authors.forename: Y Y
authors.initials: YY
authors.lastname: Liu
authors.forename: X M
authors.initials: XM
authors.lastname: Zhao
authors.forename: F Y
authors.initials: FY
authors.lastname: Liu
authors.forename: N
authors.initials: N
authors.lastname: Tian
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000166:cytology; D002448:Cell Adhesion; D004730:Endothelium, Vascular / Q000166:cytology*; D007962:Leukocytes / Q000166:cytology; D055806:Microvessels / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000473:pathology*; D014617:Vacuoles