title: Release kinetics of basic fibroblast growth factor (bFGF) from certain biopolymers in the presence of ketoprofen.
abstract: The aim of the present study was the evaluation of basic fibroblast growth factor (bFGF, FGF-2) release in vitro from four types of polymer bases (carriers), fibrin, microcrystalline chitosan (MCCh), fibrin and MCCh, as well as MCCh and methylcellulose (MC) in the presence or absence of ketoprofen (KTA). Amount of released basic fibroblast growth factor was measured immunoenzymatically using Elisa (R&D System). Ketoprofen concentration was determined spectrophoto-metrically at 255 nm, using an appropriate absorbance factor, alpha 1 cm (1%) = 662. The most significant influence of ketoprofen on bFGF release was seen in the case of microcrystalline chitosan carrier elution. Parameters of the equation which describe the amount of bFGF released from chitosan carrier with and without KTA are y = 6.842 +/- 1.637 In(t) + 14.935 +/- 2.378, determination coefficient, R2 = 0.9332 and y = 4.070 +/- 0.622 In(t) + 10.589 +/- 1.011, determination coefficient, R2 = 0.9606. The time after which 20% of bFGF was released (t 20%) in the presence of ketoprofen was 2.1 h whereas it was significantly longer without ketoprofen (10.1 h). The amount of bFGF released from fibrin carrier was lower in the presence of ketoprofen. The time taken for 20% of bFGF to be released (t 20%) was very long (41.7h) in the presence of KTA and 16.3 h. without KTA. The other carriers (fibrin + MCCh and MCCh + MC) in the presence of ketoprofen appear to have an insignificant influence on the kinetics of basic fibroblast growth factor release. For the chitosan carrier (p = 0.05, and also p = 0.01, when t(theoret) = 2.921), there is a statistically significant difference between the coefficients (a1 and a2) of the regression equation describing the process of basic fibroblast growth factor release from the base with and without ketoprofen. It was also found that the amount of ketoprofen released varied considerably according to the carrier. All results clearly indicate that the type of carrier not only has an impact on the amount of bFGF released, but also on the kinetics of ketoprofen release.
authors.affiliation: Department of Pharmaceutical Biochemistry, Faculty of Pharmacy, Medical University of Łódź, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Michalska
authors.forename: M
authors.initials: M
authors.lastname: Mirowski
authors.forename: A
authors.initials: A
authors.lastname: Bodek
authors.forename: K H
authors.initials: KH
authors.lastname: Bodek
mesh_terms: D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry*; D001704:Biopolymers; D048271:Chitosan / Q000737:chemistry; D004337:Drug Carriers; D005337:Fibrin / Q000737:chemistry; D016222:Fibroblast Growth Factor 2 / Q000008:administration & dosage* / Q000032:analysis; D007660:Ketoprofen / Q000737:chemistry*; D007700:Kinetics; D008747:Methylcellulose / Q000737:chemistry; D010592:Pharmaceutic Aids / Q000737:chemistry; D012995:Solubility
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D001704:Biopolymers; D004337:Drug Carriers; D010592:Pharmaceutic Aids; D016222:Fibroblast Growth Factor 2; D005337:Fibrin; D008747:Methylcellulose; D048271:Chitosan; D007660:Ketoprofen