title: Luciferase protein complementation assays for bioluminescence imaging of cells and mice.
abstract: Protein fragment complementation assays (PCAs) with luciferase reporters currently are the preferred method for detecting and quantifying protein-protein interactions in living animals. At the most basic level, PCAs involve fusion of two proteins of interest to enzymatically inactive fragments of luciferase. Upon association of the proteins of interest, the luciferase fragments are capable of reconstituting enzymatic activity to generate luminescence in vivo. In addition to bi-molecular luciferase PCAs, unimolecular biosensors for hormones, kinases, and proteases also have been developed using target peptides inserted between inactive luciferase fragments. Luciferase PCAs offer unprecedented opportunities to quantify dynamics of protein-protein interactions in intact cells and living animals, but successful use of luciferase PCAs in cells and mice involves careful consideration of many technical factors. This chapter discusses the design of luciferase PCAs appropriate for animal imaging, including construction of reporters, incorporation of reporters into cells and mice, imaging techniques, and data analysis.
authors.affiliation: Department of Radiology, University of Michigan, Ann Arbor, MI 48109-2200, USA. gluker@umich.edu
authors.forename: Gary D
authors.initials: GD
authors.lastname: Luker
authors.forename: Kathryn E
authors.initials: KE
authors.lastname: Luker
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D008156:Luciferases / Q000378:metabolism*; D008163:Luminescent Measurements / Q000379:methods*; D051379:Mice; D025941:Protein Interaction Mapping / Q000379:methods*
chemical_list: D008156:Luciferases