title: Human mesenchymal stem cells and renal tubular epithelial cells differentially influence monocyte-derived dendritic cell differentiation and maturation.
abstract: Mesenchymal stem cells (MSCs) possess immunosuppressive properties. But also fully differentiated human renal tubular epithelial cells (RTECs) are able to modulate T-cell proliferation in vitro. In this study we compared two MSC populations, human adipose derived stem cells (ASCs) and human amniotic mesenchymal stromal cells (hAMSCs), and RTECs regarding their potential to inhibit monocyte-derived dendritic cell (DC) differentiation and maturation in indirect co-culture. In the presence of hAMSCs and RTECs, monocytes stimulated to undergo DC differentiation were inhibited to acquire surface phenotype of immature and mature DCs. In contrast, ASCs showed only limited suppressive capacity. Secretion of IL-12p70 was suppressed in hAMSC co-cultures and high IL-10 levels were detected in all co-cultures. Prostaglandin E(2) was found in ASC and hAMSC co-cultures, whereas soluble human leukocyte antigen-G was highly elevated only in RTEC co-cultures. Thus, inhibition of DC generation by MSCs and RTECs might be mediated by different soluble factors.
authors.affiliation: Red Cross Blood Transfusion Service of Upper Austria, Krankenhausstrasse 7, A-4017 Linz, Austria. barbara.kronsteiner@o.roteskreuz.at
authors.forename: Barbara
authors.initials: B
authors.lastname: Kronsteiner
authors.forename: Anja
authors.initials: A
authors.lastname: Peterbauer-Scherb
authors.forename: Regina
authors.initials: R
authors.lastname: Grillari-Voglauer
authors.forename: Heinz
authors.initials: H
authors.lastname: Redl
authors.forename: Christian
authors.initials: C
authors.lastname: Gabriel
authors.forename: Martijn
authors.initials: M
authors.lastname: van Griensven
authors.forename: Susanne
authors.initials: S
authors.lastname: Wolbank
mesh_terms: D000328:Adult; D002454:Cell Differentiation; D002470:Cell Survival; D018920:Coculture Techniques; D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology*; D004847:Epithelial Cells / Q000276:immunology*; D005260:Female; D006680:HLA Antigens / Q000276:immunology; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I / Q000276:immunology; D006801:Humans; D016753:Interleukin-10 / Q000276:immunology / Q000378:metabolism; D018664:Interleukin-12 / Q000096:biosynthesis / Q000276:immunology; D007684:Kidney Tubules / Q000276:immunology*; D059630:Mesenchymal Stem Cells / Q000276:immunology* / Q000378:metabolism; D008875:Middle Aged; D009000:Monocytes / Q000166:cytology / Q000276:immunology*; D055815:Young Adult
chemical_list: D006680:HLA Antigens; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I; D016753:Interleukin-10; D018664:Interleukin-12