title: Effects of palmitic acid on lipid metabolism homeostasis and apoptosis in goose primary hepatocytes.
abstract: Studies have shown that not only does palmitic acid promote triglyceride (TG) accumulation, but it also affects cell viability in in vitro steatosis models. However, to what degree these effects are mediated by steatosis in goose primary hepatocytes is unknown. In this study, the effects of palmitic acid on the lipid metabolism homeostasis pathway and on apoptosis were determined. The authors measured the mRNA levels of genes involved in TG synthesis, lipid deposition, fatty acid oxidation and the assembly and secretion of VLDL-TG in goose primary hepatocytes. The results indicated that palmitic acid can significantly reduce the activity of goose hepatocytes, and that palmitic acid had a significant effect on TG accumulation; however, with increasing palmitic acid concentrations, the extracellular TG and extracellular VLDL concentration gradually decreased. With increasing palmitic acid concentrations, the gene expression levels of DGAT1, DGAT2, PPARα, CPT-1, FoxO1 and MTTP (which regulate hepatic TG synthesis, fatty acid oxidation and the assembly and secretion of VLDL-TGs) first increased and then decreased; the change in PLIN gene expression was palmitic acid dose-dependent, similar to the regulatory mode of intracellular TG accumulation. In conclusion, this study clearly shows that palmitic acid can promote TG accumulation and induce apoptosis in goose primary hepatocytes, and this effect may be related to the lipid metabolism pathway.
authors.affiliation: College of Animal Science and Technology, Sichuan Agricultural University, Ya'an, Sichuan, People's Republic of China.
authors.forename: Zhixiong
authors.initials: Z
authors.lastname: Pan
authors.forename: Jiwen
authors.initials: J
authors.lastname: Wang
authors.forename: Hui
authors.initials: H
authors.lastname: Tang
authors.forename: Liang
authors.initials: L
authors.lastname: Li
authors.forename: Jia
authors.initials: J
authors.lastname: Lv
authors.forename: Lu
authors.initials: L
authors.lastname: Xia
authors.forename: Chunchun
authors.initials: C
authors.lastname: Han
authors.forename: Feng
authors.initials: F
authors.lastname: Xu
authors.forename: Hua
authors.initials: H
authors.lastname: He
authors.forename: Hengyong
authors.initials: H
authors.lastname: Xu
authors.forename: Bo
authors.initials: B
authors.lastname: Kang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000235:genetics; D018929:Cell Culture Techniques; D016923:Cell Death / Q000187:drug effects; D002478:Cells, Cultured; D004353:Drug Evaluation, Preclinical; D005777:Geese / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D022781:Hepatocytes / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D042541:Intracellular Space / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D050356:Lipid Metabolism / Q000187:drug effects* / Q000235:genetics; D008079:Lipoproteins, VLDL / Q000032:analysis / Q000378:metabolism; D009994:Osmolar Concentration; D019308:Palmitic Acid / Q000009:adverse effects / Q000494:pharmacology*; D014280:Triglycerides / Q000032:analysis / Q000378:metabolism
chemical_list: D008079:Lipoproteins, VLDL; D014280:Triglycerides; D019308:Palmitic Acid