title: Characterization of plasma triiodophenol binding proteins in vertebrates and tissue distribution of triiodophenol in Rana catesbeiana tadpoles.
abstract: We investigated the interaction of 2,4,6-triiodophenol (TIP), a potent thyroid hormone disrupting chemical, with serum proteins from rainbow trout (Onchorhynchus mykiss), bullfrog (Rana catesbeiana), chicken (Gallus gallus), pig (Sus scrofa domesticus), and rat (Rattus norvegicus) using a [(125)I]TIP binding assay, gel filtration chromatography, and native polyacrylamide gel electrophoresis. [(125)I]TIP bound non-specifically to proteins in trout serum, specifically but weakly to proteins in bullfrog serum, and specifically and strongly to proteins in chicken, pig, and rat serum samples. Candidate TIP-binding proteins included lipoproteins (220-320kDa) in trout, albumin in bullfrog, albumin and transthyretin (TTR) in chicken and pig, and TTR in rat. TTR in the chicken, pig, and rat serum samples was responsible for the high-affinity, low-capacity binding sites for TIP (dissociation constant 2.2-3.5×10(-10)M). In contrast, a weak interaction of [(125)I]TIP with tadpole serum proteins accelerated [(125)I]TIP cellular uptake in vitro. Intraperitoneal injection of [(125)I]TIP in tadpoles revealed that the radioactivity was predominantly accumulated in the gallbladder and the kidney. The differences in the molecular and binding properties of TIP binding proteins among vertebrates would affect in part the cellular availability, tissue distribution and clearance of TIP.
authors.affiliation: Department of Biological Science, Faculty of Science, Shizuoka University, Shizuoka 422-8529, Japan. sbkyama@ipc.shizuoka.ac.jp
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Yamauchi
authors.forename: Gobun
authors.initials: G
authors.lastname: Sai
mesh_terms: D000418:Albumins / Q000378:metabolism; D000818:Animals; D002352:Carrier Proteins / Q000097:blood*; D002645:Chickens; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D007274:Injections, Intraperitoneal; D007814:Larva / Q000187:drug effects / Q000378:metabolism*; D008074:Lipoproteins / Q000378:metabolism; D008297:Male; D008565:Membrane Proteins / Q000097:blood*; D017686:Oncorhynchus mykiss; D010636:Phenols / Q000493:pharmacokinetics* / Q000633:toxicity; D011228:Prealbumin / Q000378:metabolism; D011892:Rana catesbeiana / Q000502:physiology*; D051381:Rats; D013552:Swine; D014874:Water Pollutants, Chemical / Q000493:pharmacokinetics* / Q000633:toxicity
chemical_list: D000418:Albumins; D002352:Carrier Proteins; D008074:Lipoproteins; D008565:Membrane Proteins; D010636:Phenols; D011228:Prealbumin; D014874:Water Pollutants, Chemical; C066859:2,4,6-triiodophenol