title: [Study on the integration site and arrangement of SfII and SfX prophages in Shigella flexneri serotype 2b strains].
abstract: OBJECTIVE
  To study the integration site and arrangement of SfII and SfX prophages in Shigella flexneri serotype 2b strains.
  
  
  METHODS
  A series of primers were designed based on potential integration site of SfII and SfX prophages in Shigella flexneri serotype 2b strains, and PCR were performed for 50 serotype 2b strains to amplify special genes located in host and prophages. PCR products were sequenced to identify integration sites and arrangement of SfII and SfX.
  
  
  RESULTS
  In all the serotype 2b strains, prophage SfII and SfX were adjacent to each other, and integrated into the thrW tRNA gene of the host, which were located between genes proA and yaiC of host. Prophage SfX was located immediately upstream of prophage SfII in all the detected 50 serotype 2b strains exception for strain 51251.
  
  
  CONCLUSIONS
  This was the first report on the integration site and arrangement of serotype-converting prophages SfII and SfX in Shigella flexneri 2b strains.
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
authors.forename: Yi-ting
authors.initials: YT
authors.lastname: Wang
authors.forename: Qiang-zheng
authors.initials: QZ
authors.lastname: Sun
authors.forename: Kai
authors.initials: K
authors.lastname: Liu
authors.forename: Ai-lan
authors.initials: AL
authors.lastname: Zhao
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Dong
authors.initials: D
authors.lastname: Jin
authors.forename: Zhen-jun
authors.initials: ZJ
authors.lastname: Li
authors.forename: Huai-qi
authors.initials: HQ
authors.lastname: Jing
authors.forename: Chang-yun
authors.initials: CY
authors.lastname: Ye
authors.forename: Jian-guo
authors.initials: JG
authors.lastname: Xu
mesh_terms: D004269:DNA, Bacterial / Q000235:genetics; D016680:Genome, Bacterial; D008242:Lysogeny; D039002:Prophages / Q000235:genetics* / Q000502:physiology; D017422:Sequence Analysis, DNA; D012703:Serotyping; D012763:Shigella flexneri / Q000235:genetics* / Q000821:virology*
chemical_list: D004269:DNA, Bacterial