title: Structure of the inhibitor complex of old yellow enzyme from Trypanosoma cruzi.
abstract: Old yellow enzyme (OYE) is an NADPH oxidoreductase which contains flavin mononucleotide as prosthetic group. The X-ray structures of OYE from Trypanosoma cruzi (TcOYE) which produces prostaglandin (PG) F(2α) from PGH(2) have been determined in the presence or absence of menadione. The binding motif of menadione, known as one of the inhibitors for TcOYE, should accelerate the structure-based development of novel anti-chagasic drugs that inhibit PGF(2α) production specifically.
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Osaka University, Yamada-Oka 2-1, Suita, Osaka 565-0871, Japan.
authors.forename: Keishi
authors.initials: K
authors.lastname: Yamaguchi
authors.forename: Naoki
authors.initials: N
authors.lastname: Okamoto
authors.forename: Keiji
authors.initials: K
authors.lastname: Tokuoka
authors.forename: Shigeru
authors.initials: S
authors.lastname: Sugiyama
authors.forename: Nahoko
authors.initials: N
authors.lastname: Uchiyama
authors.forename: Hiroyoshi
authors.initials: H
authors.lastname: Matsumura
authors.forename: Koji
authors.initials: K
authors.lastname: Inaka
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Urade
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Inoue
mesh_terms: D001665:Binding Sites; D018360:Crystallography, X-Ray; D005486:Flavin Mononucleotide / Q000737:chemistry; D006911:Hydroxyprostaglandin Dehydrogenases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D008958:Models, Molecular; D009252:NADPH Dehydrogenase / Q000037:antagonists & inhibitors* / Q000737:chemistry*; D014349:Trypanosoma cruzi / Q000201:enzymology*; D024483:Vitamin K 3 / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D024483:Vitamin K 3; D005486:Flavin Mononucleotide; D006911:Hydroxyprostaglandin Dehydrogenases; C031051:prostaglandin-F synthase; D009252:NADPH Dehydrogenase