title: An amperometric oxalate biosensor based on sorghum oxalate oxidase bound carboxylated multiwalled carbon nanotubes-polyaniline composite film.
abstract: A highly sensitive, specific and rapid electrochemical oxalate biosensor was constructed by covalently immobilizing sorghum leaf oxalate oxidase on carboxylated multiwalled carbon nanotubes and conducting polymer, polyaniline nanocomposite film electrodeposited over the surface of platinum (Pt) wire using N-ethyl-N'-(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxy succinimide (NHS) chemistry. The modified electrode was characterized by scanning electron microscopy (SEM) and Fourier transform infrared (FTIR) spectrophotometry. The optimized oxalate biosensor showed linear response range of 8.4-272 μM with correlation coefficient of 0.93 and rapid response within 5s at a potential of 0.4V vs Ag/AgCl. The sensitivity was approximately 0.0113 μA/μM with a detection limit of 3.0 μM. Proposed oxalate biosensor was successfully applied to human urine sample.
authors.affiliation: Department of Biochemistry, Maharshi Dayanand University, Rohtak 124001, Haryana, India.
authors.forename: Sandeep
authors.initials: S
authors.lastname: Yadav
authors.forename: Rooma
authors.initials: R
authors.lastname: Devi
authors.forename: Santosh
authors.initials: S
authors.lastname: Kumari
authors.forename: Sarita
authors.initials: S
authors.lastname: Yadav
authors.forename: C S
authors.initials: CS
authors.lastname: Pundir
mesh_terms: D000814:Aniline Compounds / Q000737:chemistry*; D015374:Biosensing Techniques; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D005022:Ethyldimethylaminopropyl Carbodiimide / Q000737:chemistry; D006801:Humans; D008855:Microscopy, Electron, Scanning / Q000379:methods; D053761:Nanocomposites / Q000737:chemistry; D036103:Nanotechnology / Q000379:methods*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D010088:Oxidoreductases / Q000737:chemistry*; D045868:Sorghum / Q000201:enzymology*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D013388:Succinimides / Q000737:chemistry; D016482:Urinalysis / Q000295:instrumentation* / Q000379:methods
chemical_list: D000814:Aniline Compounds; D037742:Nanotubes, Carbon; D013388:Succinimides; C416807:polyaniline; D010088:Oxidoreductases; C020598:oxalate oxidase; C001426:N-hydroxysuccinimide; D005022:Ethyldimethylaminopropyl Carbodiimide