title: Antifungal mechanisms by which a novel Pseudomonas aeruginosa phenazine toxin kills Candida albicans in biofilms.
abstract: Pseudomonas aeruginosa produces several phenazines including the recently described 5-methyl-phenazine-1-carboxylic acid (5MPCA), which exhibits a novel antibiotic activity towards pathogenic fungi such as Candida albicans. Here we characterize the unique antifungal mechanisms of 5MPCA using its analogue phenazine methosulphate (PMS). Like 5MPCA, PMS induced fungal red pigmentation and killing. Mass spectrometry analyses demonstrated that PMS can be covalently modified by amino acids, a process that yields red derivatives. Furthermore, soluble proteins from C. albicans grown with either PMS or P. aeruginosa were also red and demonstrated absorbance and fluorescence spectra similar to that of PMS covalently linked to either amino acids or proteins in vitro, suggesting that 5MPCA modification by protein amine groups occurs in vivo. The red-pigmented C. albicans soluble proteins were reduced by NADH and spontaneously oxidized by oxygen, a reaction that likely generates reactive oxygen species (ROS). Additional evidence indicated that ROS generation precedes 5MPCA-induced fungal death. Reducing conditions greatly enhanced PMS uptake by C. albicans and killing. Since 5MPCA was more toxic than other phenazines that are not modified, such as pyocyanin, we propose that the covalent binding of 5MPCA promotes its accumulation in target cells and contributes to its antifungal activity in mixed-species biofilms.
authors.affiliation: Department of Microbiology and Immunology, Dartmouth Medical School, Hanover, NH 03755, USA.
authors.forename: Diana K
authors.initials: DK
authors.lastname: Morales
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: Jacobs
authors.forename: Sathish
authors.initials: S
authors.lastname: Rajamani
authors.forename: Malathy
authors.initials: M
authors.lastname: Krishnamurthy
authors.forename: Juan R
authors.initials: JR
authors.lastname: Cubillos-Ruiz
authors.forename: Deborah A
authors.initials: DA
authors.lastname: Hogan
mesh_terms: D000935:Antifungal Agents / Q000378:metabolism* / Q000494:pharmacology; D001427:Bacterial Toxins / Q000378:metabolism* / Q000494:pharmacology; D018441:Biofilms; D002176:Candida albicans / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D008773:Methylphenazonium Methosulfate / Q000378:metabolism* / Q000494:pharmacology; D050296:Microbial Viability; D015394:Molecular Structure; D010084:Oxidation-Reduction; D011550:Pseudomonas aeruginosa / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D000935:Antifungal Agents; D001427:Bacterial Toxins; D005656:Fungal Proteins; D017382:Reactive Oxygen Species; D008773:Methylphenazonium Methosulfate