title: Mammalian ER stress sensor IRE1β specifically down-regulates the synthesis of secretory pathway proteins.
abstract: Accumulation of unfolded proteins in the endoplasmic reticulum (ER) causes ER stress. The ER stress sensor inositol requiring enzyme-1beta (IRE1β), which is specifically expressed in intestinal epithelial cells, is thought to be involved in translational repression. However, its mechanism of action is not fully understood. Using a reporter that can evaluate and distinguish between translation efficiency in the cytosol and on the ER membrane, we show here that IRE1β represses translation on the ER membrane but not in the cytosol, and that this selective repression depends on the RNase activity of IRE1β.
authors.affiliation: Laboratory of Molecular and Cell Genetics, Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Nara, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Nakamura
authors.forename: Akio
authors.initials: A
authors.lastname: Tsuru
authors.forename: Kentaro
authors.initials: K
authors.lastname: Ikegami
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Imagawa
authors.forename: Naoko
authors.initials: N
authors.lastname: Fujimoto
authors.forename: Kenji
authors.initials: K
authors.lastname: Kohno
mesh_terms: D015153:Blotting, Western; D003600:Cytosol / Q000378:metabolism; D015536:Down-Regulation; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D004722:Endoribonucleases / Q000235:genetics / Q000378:metabolism*; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D014176:Protein Biosynthesis; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D021381:Protein Transport; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012260:Ribonucleases / Q000378:metabolism; D055571:Secretory Pathway; D013312:Stress, Physiological
chemical_list: D006023:Glycoproteins; D008565:Membrane Proteins; D012333:RNA, Messenger; C403982:ERN2 protein, human; D017346:Protein Serine-Threonine Kinases; D004722:Endoribonucleases; D012260:Ribonucleases