title: [Efficient polyhydroxybutyrate production from cheap resources by recombinant Escherichia coli].
abstract: Based on the fermentation analysis of Escherichia coli strains and cheap renewable resources suitable for polyhydroxybutyrate (PHB) production, we constructed a ptsG mutant of Escherichia coli DH5alpha. Application of E. coli DH5alpha mutant together with stress-induced system, we could produce PHB efficiently from cheap renewable sugar mixture by the simultaneous consumption of different sugars. Batch fermentation at lab scale (5 liter) showed that E. coli DH5alpha deltaptsG/pQKZ103 produced PHB from sugar mixture up to 84.6% of cell dry weight in 32 hours; meanwhile, the cell dry weight reached 8.24 g/L.
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China.
authors.forename: Guoqing
authors.initials: G
authors.lastname: Wei
authors.forename: Quan
authors.initials: Q
authors.lastname: Chen
authors.forename: Zhen
authors.initials: Z
authors.lastname: Kang
authors.forename: Qingsheng
authors.initials: Q
authors.lastname: Qi
mesh_terms: D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D005285:Fermentation; D005822:Genetic Vectors / Q000235:genetics*; D006885:Hydroxybutyrates / Q000378:metabolism*; D060847:Metabolic Engineering / Q000379:methods*; D009154:Mutation; D011091:Polyesters / Q000378:metabolism*; D000094122:Polyhydroxybutyrates
chemical_list: D006885:Hydroxybutyrates; D011091:Polyesters; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates