title: [Cloning of B7-1 and B7-2 genes from human Burkitt's B lymphocyte line].
abstract: OBJECTIVE
  In order to construct recombinant B7-Pseudomonas exotoxin fusion protein to induce long-term immunotolerance, we tried to clone both B7-1 and B7-2 genes from human Burkitt's B lymphocyte line (Raji) and construct the recombinant plasmids encoding for B7-1 and B7-2.
  
  
  METHODS
  Two pairs of primers for B7-1 and B7-2 were designed and synthesized according to the sequences o human B7-1 and B7-2 genes derived from genbank. Two recombinant plasmids pGEM-T-B7-1 land pGEM-T-B7-2 were constructed by recombinant gene techniques.
  
  
  CONCLUSIONS
  We have successfully cloned both B7-1 and B7-2 cDNA-which, confirmed by DNA sequencing, EcoRI/Hind III and BamHI/Sph1 restriction enzyme digesting, could be used to construct recombinant B7-Pseudomonas exotoxin fusion proteins.
authors.affiliation: Department of Immunology, Affiliated Hospital for Academy of Medical Sciences, Beijing 100039, China.
authors.forename: H L
authors.initials: HL
authors.lastname: Zhang
authors.forename: Y Z
authors.initials: YZ
authors.lastname: Xi
authors.forename: F H
authors.initials: FH
authors.lastname: Kong
mesh_terms: D018122:B7-1 Antigen / Q000235:genetics*; D051940:B7-2 Antigen / Q000235:genetics*; D045744:Cell Line, Tumor; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D010957:Plasmids / Q000235:genetics
chemical_list: D018122:B7-1 Antigen; D051940:B7-2 Antigen; D018076:DNA, Complementary