title: [Expression of ryanodine receptor isoforms in airway smooth muscle cells of rats].
abstract: OBJECTIVE
  To detect the expression of ryanodine receptor (RyR) subtypes in normol rat airway smooth muscle cells(ASMCs) and changes during chronic asthma formation.
  
  
  METHODS
  ASMCs were cultured with collagen enzyme digestion method. The expression of subtypes of RyR were detected by RT-PCR. Purified PCR product linked with pGEM-T vector to make DNA sequence assay. Chronic asthma model was made with OVA, the changes of RyRs detected by RT-PCR.
  
  
  RESULTS
  All subtypes of RyR were expressed in airway smooth muscle cells of normol rat. The expression of RyR1 increased obviously compared with control group (P < 0.05) on chronic asthma.
  
  
  CONCLUSIONS
  Co-expression of three subtypes of RyR in ASMCs of normal rat, indicate that there are complicated intercellular Ca2+ regulation mechanism in ASM, moreover RyR1 might play a role during asthma development.
authors.affiliation: Institute of Respiratory Medicine, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.forename: Sui-yang
authors.initials: SY
authors.lastname: Zhang
authors.forename: Xi-liang
authors.initials: XL
authors.lastname: Wang
authors.forename: Gui-sheng
authors.initials: GS
authors.lastname: Qian
mesh_terms: D000818:Animals; D001249:Asthma / Q000378:metabolism*; D001980:Bronchi; D008297:Male; D032389:Myocytes, Smooth Muscle / Q000378:metabolism*; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D019837:Ryanodine Receptor Calcium Release Channel / Q000235:genetics / Q000378:metabolism*
chemical_list: D020033:Protein Isoforms; D012333:RNA, Messenger; D019837:Ryanodine Receptor Calcium Release Channel