title: Establishment of ku70-deficient lung epithelial cell lines and their hypersensitivity to low-dose x-irradiation.
abstract: In clinical situations, cellular resistance to chemotherapy and radiotherapy is a significant component of tumor treatment failure. The DNA repair protein Ku70 is a key contributor to chemoresistance to anticancer agents, e.g., etoposide and bleomycin, or radioresistance. Ku70 plays a key role as a sensor of DNA double-strand breaks (DSBs) induced following exposure to ionizing radiation as well as treatment with some chemotherapeutic drugs. The responses of different organs to radiation vary widely and likely depend on the cell population in the organs. However, it is not clear whether Ku70 plays a role in the low-dose radioresistance of lung epithelial cells. In this study, we established Ku70-deficient epithelial cell lines from murine lungs lacking Ku70. Ku70-/- lung epithelial cells exhibited reduced Ku80 expression. Moreover, Ku70-/- lung epithelial cells were more sensitive than controls (Ku70+/- lung epithelial cells) to low-dose X-irradiation (< 0.5 Gy). We also found that consistent with the Ku70 function as a sensor of DSBs, Ku70 mainly localized in the nuclei of murine lung epithelial cells. These findings clearly indicate that Ku70 plays a key role in regulation of the Ku80 expression level in and the radioresistance of lung epithelial cells. Our data also suggest that these cell lines might be useful not only for study of Ku70 functions and the DSB repair pathway, but also for study of the molecular mechanism underlying the sensitivity to chemotherapeutic drugs and radiation in lung epithelial cells.
authors.affiliation: DNA Repair Gene Res., National Institute of Radiological Sciences, 4–9–1 Anagawa, Inage-ku, Chiba 263–8555. m_koike@nirs.go.jp
authors.forename: Manabu
authors.initials: M
authors.lastname: Koike
authors.forename: Yasutomo
authors.initials: Y
authors.lastname: Yutoku
authors.forename: Aki
authors.initials: A
authors.lastname: Koike
mesh_terms: D000818:Animals; D034961:Antigens, Nuclear / Q000235:genetics* / Q000378:metabolism; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects*; D005786:Gene Expression Regulation; D006801:Humans; D000072200:Ku Autoantigen; D008168:Lung / Q000166:cytology*; D051379:Mice; D018345:Mice, Knockout; D020545:Respiratory Mucosa / Q000166:cytology*; D014965:X-Rays / Q000009:adverse effects*
chemical_list: D034961:Antigens, Nuclear; D004268:DNA-Binding Proteins; C000606509:Xrcc6 protein, human; C000606510:Xrcc6 protein, mouse; D000072200:Ku Autoantigen