title: Gene clusters reflecting macrodomain structure respond to nucleoid perturbations.
abstract: Focusing on the DNA-bridging nucleoid proteins Fis and H-NS, and integrating several independent experimental and bioinformatic data sources, we investigate the links between chromosomal spatial organization and global transcriptional regulation. By means of a novel multi-scale spatial aggregation analysis, we uncover the existence of contiguous clusters of nucleoid-perturbation sensitive genes along the genome, whose expression is affected by a combination of topological DNA state and nucleoid-shaping protein occupancy. The clusters correlate well with the macrodomain structure of the genome. The most significant of them lay symmetrically at the edges of the Ter macrodomain and involve all of the flagellar and chemotaxis machinery, in addition to key regulators of biofilm formation, suggesting that the regulation of the physical state of the chromosome by the nucleoid proteins plays an important role in coordinating the transcriptional response leading to the switch between a motile and a biofilm lifestyle.
authors.affiliation: Genomic Physics Group, FRE 3214 CNRS Microorganism Genomics, France.
authors.forename: Vittore F
authors.initials: VF
authors.lastname: Scolari
authors.forename: Bruno
authors.initials: B
authors.lastname: Bassetti
authors.forename: Bianca
authors.initials: B
authors.lastname: Sclavi
authors.forename: Marco Cosentino
authors.initials: MC
authors.lastname: Lagomarsino
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D018441:Biofilms / Q000254:growth & development; D004269:DNA, Bacterial / Q000737:chemistry* / Q000235:genetics*; D004278:DNA, Superhelical / Q000737:chemistry / Q000235:genetics; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D035021:Factor For Inversion Stimulation Protein / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017353:Gene Deletion; D016680:Genome, Bacterial / Q000235:genetics; D005810:Multigene Family / Q000235:genetics*; D009690:Nucleic Acid Conformation; D020411:Oligonucleotide Array Sequence Analysis; D011487:Protein Conformation; D014158:Transcription, Genetic / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004278:DNA, Superhelical; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D035021:Factor For Inversion Stimulation Protein; C501034:Fis protein, E coli; C031908:H-NS protein, bacteria