title: Differential effects of platelet rich plasma and washed platelets on the proliferation of mouse MSC cells.
abstract: Multipotent mesenchymal stem cell (MSC) therapies are being tested clinically for a variety of disorders. However, despite the remarkable clinical advancements in this field, most applications still use traditional culture media containing fetal bovine serum. Platelet-rich plasma (PRP) appears as a novel application for tissue engineering and its effect on bone healing is thought to be mainly dependent on the proliferation promoting function, with the molecular mechanisms largely unknown. In this study, mouse osteogenic progenitor mesenchymal stem cells (MSCs) were cultured in PRP or washed platelet (WPLT)-treated wells or in untreated wells, and analyzed on cycloxygenase 2 (COX2) expression (qRT-PCR), cell growth (MTT assay) and cell differentiation (alkaline phosphatase activity). The results showed that PRP and WPLT stimulated cell growth similarly in the first 6 days, together with the steady induction of COX2 and PGE2. 10 μmol/l celecoxib (an inhibitor of COX2) significantly inhibited the pro-proliferation effects. Interestingly, WPLT had stronger effects than PRP in proliferation at the later time points (6-9 days). ALP activity assay and collagen 1a expression revealed PRP had a mild but statistically significant pro-differentiation effect, while no obvious effects observed in WLPT group. In summary, PRP stimulates initial growth of MSCs in a COX2 partially dependent manner and the less obvious osteogenic differentiation promoting effects of WPLT strongly indicates WPLT rather than the PRP should be the optional choice for expanding MSCs in vitro for clinical use.
authors.affiliation: Department of Stomatology, Guangzhou General Hospital of Guangzhou Military Command, 111 Liuhua Road, 510010, Guangzhou, People's Republic of China.
authors.forename: Jianmin
authors.initials: J
authors.lastname: Duan
authors.forename: Wei
authors.initials: W
authors.lastname: Kuang
authors.forename: Jiali
authors.initials: J
authors.lastname: Tan
authors.forename: Hongtao
authors.initials: H
authors.lastname: Li
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Kikuchi
authors.initials: K
authors.lastname: Hirotaka
authors.forename: Katayama
authors.initials: K
authors.lastname: Tadashi
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001792:Blood Platelets / Q000502:physiology*; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation / Q000502:physiology; D049109:Cell Proliferation; D051546:Cyclooxygenase 2 / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D051379:Mice; D053657:Platelet-Rich Plasma / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013778:Tetrazolium Salts; D013844:Thiazoles; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D017931:DNA Primers; D013778:Tetrazolium Salts; D013844:Thiazoles; D051546:Cyclooxygenase 2; D000469:Alkaline Phosphatase; C022616:thiazolyl blue