title: Identification of a new isoform of eEF2 whose phosphorylation is required for completion of cell division in sea urchin embryos.
abstract: Elongation factor 2 (eEF2) is the main regulator of peptide chain elongation in eukaryotic cells. Using sea urchin eggs and early embryos, two isoforms of eEF2 of respectively 80 and 83 kDa apparent molecular weight have been discovered. Both isoforms were identified by immunological analysis as well as mass spectrometry, and appeared to originate from a unique post-translationally modified protein. Accompanying the net increase in protein synthesis that occurs in early development, both eEF2 isoforms underwent dephosphorylation in the 15 min period following fertilization, in accordance with the active role of dephosphorylated eEF2 in regulation of protein synthesis. After initial dephosphorylation, the major 83 kDa isoform remained dephosphorylated while the 80 kDa isoform was progressively re-phosphorylated in a cell-cycle dependent fashion. In vivo inhibition of phosphorylation of the 80 kDa isoform impaired the completion of the first cell cycle of early development implicating the involvement of eEF2 phosphorylation in the exit from mitosis.
authors.affiliation: Centre National de la Recherche Scientifique, UMR 7150 Mer & Santé, Equipe Traduction Cycle Cellulaire et Développement, Roscoff, France.
authors.forename: Robert
authors.initials: R
authors.lastname: Bellé
authors.forename: Pierre-François
authors.initials: PF
authors.lastname: Pluchon
authors.forename: Patrick
authors.initials: P
authors.lastname: Cormier
authors.forename: Odile
authors.initials: O
authors.lastname: Mulner-Lorillon
mesh_terms: D000818:Animals; D002455:Cell Division; D005306:Fertilization; D008970:Molecular Weight; D010063:Ovum / Q000737:chemistry; D020652:Peptide Elongation Factor 2 / Q000502:physiology*; D010766:Phosphorylation; D020033:Protein Isoforms; D012617:Sea Urchins / Q000196:embryology*
chemical_list: D020652:Peptide Elongation Factor 2; D020033:Protein Isoforms