title: Hydractinia echinata test system. II. SAR toxicity study of some anilide derivatives of Naphthol-AS type.
abstract: In this paper, a toxicity study for a series of anilides of Naphthol-AS type is presented. The toxicity of the model compounds was determined by using the Hydractinia echinata (Hydrozoa) test system. Conformational analysis of Naphthol-AS derivatives was performed to elucidate the possible enzymatic hydrolysis mechanism of these compounds. This mechanism occurs with different rates and always leads to a stoichiometric mixture of reaction products, consisting in the substituted amine and the corresponding α-hydroxy-carboxylic acid. With one exception, the toxicities of the reaction products are subadditive. Quite similar measured toxicity values, log(1/MRC₅₀), led to their average calculated values, and thus to the establishment of class isotoxicity. This method represents a practical alternative useful for the reduction of experimental tests on animals to the lowest possible level, in accordance to the '3Rs' (reduction, refinement and replacement) concept.
authors.affiliation: Institute of Chemistry Timişoara of the Romanian Academy, B-dul Mihai Viteazul 24, RO-300223 Timişoara, Romania. lchi@gmx.de
authors.forename: Sergiu Adrian
authors.initials: SA
authors.lastname: Chicu
authors.forename: Simona
authors.initials: S
authors.lastname: Funar-Timofei
authors.forename: Georgeta-Maria
authors.initials: GM
authors.lastname: Simu
mesh_terms: D000818:Animals; D037402:Hydrozoa / Q000187:drug effects*; D009284:Naphthols / Q000737:chemistry / Q000633:toxicity*; D013329:Structure-Activity Relationship; D018675:Toxicity Tests / Q000379:methods*; D014874:Water Pollutants, Chemical / Q000737:chemistry / Q000633:toxicity*
chemical_list: D009284:Naphthols; D014874:Water Pollutants, Chemical; C008584:naphthol AS