title: The interaction of unfolding α-lactalbumin and malate dehydrogenase with the molecular chaperone αB-crystallin: a light and X-ray scattering investigation.
abstract: OBJECTIVE
  The molecular chaperone αB-crystallin is found in high concentrations in the lens and is present in all major body tissues. Its structure and the mechanism by which it protects its target protein from aggregating and precipitating are not known.
  
  
  METHODS
  Dynamic light scattering and X-ray solution scattering techniques were used to investigate structural features of the αB-crystallin oligomer when complexed with target proteins under mild stress conditions, i.e., reduction of α-lactalbumin at 37 °C and malate dehydrogenase when heated at 42 °C. In this investigation, the size, shape and particle distribution of the complexes were determined in real-time following the induction of stress.
  
  
  RESULTS
  Overall, it is observed that the mass distribution, hydrodynamic radius, and spherical shape of the αB-crystallin oligomer do not alter significantly when it complexes with its target protein.
  
  
  CONCLUSIONS
  The data are consistent with the target protein being located in the outer protein shell of the αB-crystallin oligomer where it is readily accessible for possible refolding via the action of other molecular chaperones.
authors.affiliation: School of Optometry and Vision Sciences, Cardiff University, Cardiff, UK. reginijw@cf.ac.uk
authors.forename: Justyn W
authors.initials: JW
authors.lastname: Regini
authors.forename: Heath
authors.initials: H
authors.lastname: Ecroyd
authors.forename: Sarah
authors.initials: S
authors.lastname: Meehan
authors.forename: Kristen
authors.initials: K
authors.lastname: Bremmell
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Clarke
authors.forename: Donna
authors.initials: D
authors.lastname: Lammie
authors.forename: Tim
authors.initials: T
authors.lastname: Wess
authors.forename: John A
authors.initials: JA
authors.lastname: Carver
mesh_terms: D000818:Animals; D002417:Cattle; D006801:Humans; D007768:Lactalbumin / Q000737:chemistry / Q000378:metabolism*; D008027:Light; D008291:Malate Dehydrogenase / Q000737:chemistry / Q000378:metabolism*; D020836:Protein Structure, Quaternary; D058767:Protein Unfolding; D012542:Scattering, Radiation; D034421:Sus scrofa; D013696:Temperature; D014965:X-Rays; D038203:alpha-Crystallin B Chain / Q000737:chemistry / Q000378:metabolism*
chemical_list: D038203:alpha-Crystallin B Chain; D007768:Lactalbumin; D008291:Malate Dehydrogenase