title: Gcn5 regulates the dissociation of SWI/SNF from chromatin by acetylation of Swi2/Snf2.
abstract: The positive link between the SWI/SNF and the Gcn5 histone acetyltransferase in transcriptional activation has been well described. Here we report an inhibitory role for Gcn5 in SWI/SNF targeting. We demonstrate that Gcn5-containing complexes directly acetylate the Snf2 subunit of the SWI/SNF complex in vitro, as well as in vivo. Moreover, the acetylation of Snf2 facilitates the dissociation of the SWI/SNF complex from acetylated histones, and reduces its association with promoters in vivo. These data reveal a novel mechanism by which Gcn5 modulates chromatin structure not only through the acetylation of histones, but also by directly acetylating Snf2.
authors.affiliation: Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA.
authors.forename: Jeong-Hoon
authors.initials: JH
authors.lastname: Kim
authors.forename: Anita
authors.initials: A
authors.lastname: Saraf
authors.forename: Laurence
authors.initials: L
authors.lastname: Florens
authors.forename: Michael
authors.initials: M
authors.lastname: Washburn
authors.forename: Jerry L
authors.initials: JL
authors.lastname: Workman
mesh_terms: D000107:Acetylation; D000251:Adenosine Triphosphatases / Q000378:metabolism*; D002843:Chromatin / Q000378:metabolism*; D005656:Fungal Proteins / Q000378:metabolism* / Q000502:physiology; D051548:Histone Acetyltransferases / Q000502:physiology*; D006657:Histones / Q000378:metabolism; D046912:Multiprotein Complexes / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism* / Q000502:physiology*; D014157:Transcription Factors / Q000378:metabolism*; D000099224:p300-CBP-Associated Factor
chemical_list: D000251:Adenosine Triphosphatases; D002843:Chromatin; D005656:Fungal Proteins; D051548:Histone Acetyltransferases; D006657:Histones; D046912:Multiprotein Complexes; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; C501392:GCN5 protein, S cerevisiae; D000099224:p300-CBP-Associated Factor; C499333:SNF2 protein, S cerevisiae