title: Immunoregulatory gene polymorphisms in women with preeclampsia.
abstract: The costimulatory molecules CD28, cytotoxic T-lymphocyte antigen-4 (CTLA-4) (cytotoxic T-lymphocyte-associated antigen-4) and inducible costimulator (ICOS) are believed to have a critical modulatory role in the immune response. However, few studies have been performed on the role of these immune regulatory molecules and their polymorphisms in women with preeclampsia (PE). The aim of our study was to evaluate the CTLA4 (+49 A/G) (rs 231775), CD28 (+17 T/C) (rs 3116496) and ICOS (-1564 T/C) (rs 4675378) gene polymorphisms in Brazilian women with PE. This case-control study included 130 patients with PE and 261 control women without any obstetric or systemic disorders. Genomic DNA was extracted from peripheral blood, and the polymorphism genotyping was performed by digesting the PCR products with the restriction endonucleases BbvI (CTLA-4), AfeI (CD28) and AluI (ICOS). Data were analyzed by χ(2) or Fisher's exact test; a P-value of <0.05 was considered as significant. There were significant differences in the ICOS genotype and allelic frequencies between the PE and control groups (P=0.01 and P=0.01, respectively). We found a significantly lower frequency of the ICOS (-1564) T allele in women with mild PE compared with the controls. There were no differences in the CTLA-4 (+49 A/G) and CD28 (+17 T/C) genotypes and allelic frequencies between the PE patients and controls. Our data suggest that PE is associated with ICOS, but is not associated with the CTLA-4 or CD28 gene polymorphisms.
authors.affiliation: Department of Obstetrics, São Paulo Federal University, São Paulo, Brazil.
authors.forename: Karen P T
authors.initials: KP
authors.lastname: Pendeloski
authors.forename: Nelson
authors.initials: N
authors.lastname: Sass
authors.forename: Maria R
authors.initials: MR
authors.lastname: Torloni
authors.forename: Rosiane
authors.initials: R
authors.lastname: Mattar
authors.forename: Antonio F
authors.initials: AF
authors.lastname: Moron
authors.forename: Camila S
authors.initials: CS
authors.lastname: Franchim
authors.forename: Silvia
authors.initials: S
authors.lastname: Daher
mesh_terms: D000328:Adult; D015703:Antigens, CD / Q000235:genetics* / Q000276:immunology; D000945:Antigens, Differentiation, T-Lymphocyte / Q000235:genetics* / Q000276:immunology; D001938:Brazil / Q000453:epidemiology; D018106:CD28 Antigens / Q000235:genetics* / Q000276:immunology; D060908:CTLA-4 Antigen; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006801:Humans; D060889:Inducible T-Cell Co-Stimulator Protein; D011110:Polymorphism, Genetic; D011225:Pre-Eclampsia / Q000453:epidemiology / Q000235:genetics* / Q000276:immunology; D011247:Pregnancy; D055815:Young Adult
chemical_list: D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D018106:CD28 Antigens; D060908:CTLA-4 Antigen; C556706:CTLA4 protein, human; C556692:ICOS protein, human; D060889:Inducible T-Cell Co-Stimulator Protein