title: Isoprostanes-biomarkers of lipid peroxidation: their utility in evaluating oxidative stress and analysis.
abstract: Isoprostanes (IsoPs) are key biomarkers for investigating the role of free radical generation in the pathogenesis of human disorders. To solve IsoPs-related problems with regard to isoprostanes, analytical tools are required. This paper reviews the problems and trends in this field focusing on the methodology for assaying biomarkers in exhaled breath condensate (EBC) samples. A large amount of work has been done in the qualitative and quantitative analysis of IsoPs, but a standardized method has yet to emerge. The methodologies described differ, either in the sample preparation steps or in the detection techniques, or both. Requiring a number of chromatographic steps, the relevant extraction and purification procedures are often critical and time-consuming, and they lead to a substantial loss of target compounds. Recent data show that EBC is a promising non-invasive tool for the evaluation of different diseases. Two main analytical approaches have been adopted for IsoPs measurement: immunological methods and mass spectrometry. The methodologies for the extraction, purification and analysis of IsoPs in EBC samples are presented.
authors.affiliation: Department of Analytical Chemistry, Chemical Faculty, Gdańsk University of Technology, Narutowicza 11/12, 80-233 Gdańsk, Poland; E-Mails: agata@chem.pg.gda.pl (A.K.-W.); jacek.namiesnik@pg.gda.pl (J.N.).
authors.forename: Monika
authors.initials: M
authors.lastname: Janicka
authors.forename: Agata
authors.initials: A
authors.lastname: Kot-Wasik
authors.forename: Jacek
authors.initials: J
authors.lastname: Kot
authors.forename: Jacek
authors.initials: J
authors.lastname: Namieśnik
mesh_terms: D015415:Biomarkers / Q000032:analysis; D001944:Breath Tests / Q000379:methods; D006801:Humans; D028421:Isoprostanes / Q000032:analysis*; D015227:Lipid Peroxidation; D018384:Oxidative Stress; D012140:Respiratory Tract Diseases / Q000175:diagnosis / Q000378:metabolism
chemical_list: D015415:Biomarkers; D028421:Isoprostanes