title: The effect of loading icariin on biocompatibility and bioactivity of porous β-TCP ceramic.
abstract: In order to enhance the ability of calcium phosphate-based biomaterials for bone defect repair, icariin (Ica), one natural product with ability of promoting osteoblasts differentiation in vitro and enhancing bone formation in vivo, was loaded into porous β-tricalcium phosphate ceramic (β-TCP) disks. The obtained Ica-loaded porous β-TCP ceramic (Ica/β-TCP) disks were characterized by SEM. The SEM photos indicated that the disks had porous structure and the surface morphology of the porous β-TCP ceramic (β-PTCP) disks had no obvious difference from the Ica/β-TCP disks. The Ica release curve of Ica/β-TCP disks showed a burst release during the first 1 day and the concentration of released Ica during the first 3 days had low cytotoxicity. The loading Ica in Ica/β-TCP disks hardly affected the attachment and morphology of Ros17/28 cells, however, the Ica/β-TCP disks were favorable to supporting the proliferation and differentiation of Ros17/28 cells better compared with the β-PTCP disks. There was plenty of bone-like apatite formed on the surface of Ica/β-TCP disks soaked in SBF solution for three days. After back intramuscular implantation of rats for three months, no obvious osteogenic evidence was detected in β-PTCP disks, but new bone formation was observed in Ica/β-TCP disks. Fibrous tissues and slight inflammatory reaction was also found in the Ica/β-TCP disks and β-TCP disks. Therefore, the loading Ica did not change the biocompatibility of β-TCP ceramic, but enhanced the bioactivity of β-TCP ceramic in vivo. The Ica/β-TCP ceramic had potential to be used for bone defect repair.
authors.affiliation: Engineering Research Center in Biomaterials, Sichuan University, 610064, Chengdu, China.
authors.forename: X
authors.initials: X
authors.lastname: Zhang
authors.forename: Y
authors.initials: Y
authors.lastname: Guo
authors.forename: D X
authors.initials: DX
authors.lastname: Li
authors.forename: R
authors.initials: R
authors.lastname: Wang
authors.forename: H S
authors.initials: HS
authors.lastname: Fan
authors.forename: Y M
authors.initials: YM
authors.lastname: Xiao
authors.forename: L
authors.initials: L
authors.lastname: Zhang
authors.forename: X D
authors.initials: XD
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002130:Calcium Phosphates / Q000737:chemistry* / Q000494:pharmacology; D002454:Cell Differentiation; D002460:Cell Line; D049109:Cell Proliferation; D002516:Ceramics / Q000737:chemistry; D005419:Flavonoids / Q000008:administration & dosage*; D051379:Mice; D008855:Microscopy, Electron, Scanning / Q000379:methods; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism; D016062:Porosity; D013499:Surface Properties; D013997:Time Factors; D023822:Tissue Engineering / Q000379:methods
chemical_list: D001672:Biocompatible Materials; D002130:Calcium Phosphates; D005419:Flavonoids; C485817:beta-tricalcium phosphate; C020243:calcium phosphate; C056599:icariin