title: [Proteomics analysis on stressed myocardium injury-related proteins].
abstract: OBJECTIVE
  To probe the related proteins to stress-induced myocardium injury.
  
  
  METHODS
  After establishment of a myocardium injury model induced by restraint stress in rats, myocardium proteins of restraint stress-treated and untreated rats were extracted, and the two-dimensional polyacrylamide gel electrophoresis (2-DE) maps of the extracted proteins were established by using the immobilized pH gradient (IPG) and SDS-PAGE two-dimensional electrophoresis respectively. The alterative protein spots were analyzed by Image Master 3.01 software and identified with assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and database searching.
  
  
  RESULTS
  Proteomics analysis showed that there were 10 proteins were significantly influenced by restraint stress in rat myocardium. After stress, proteins, including cardiac myosin heavy chain, dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex, similar to dihydrolipoamide S-succinyltransferase, mitochondrial aldehyde dehydrogenase, H (+)-transporting ATP synthase, albumin, myosin heavy chain and apolipoprotein A-I precursor showed increased expression. Mitochondrial aconitase and uncoupling protein UCP-3 showed decreased expression.
  
  
  CONCLUSIONS
  These differential expressive proteins might be involved in stress-induced injury to myocardium.
authors.affiliation: Institute of Health and Environmental Medicine, Academy of Military Medical Sciences, 300050 Tianjin, China.
authors.forename: Jing-bo
authors.initials: JB
authors.lastname: Gong
authors.forename: Shu-qing
authors.initials: SQ
authors.lastname: Wu
authors.forename: Ling-jia
authors.initials: LJ
authors.lastname: Qian
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D006360:Heat-Shock Proteins / Q000378:metabolism*; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D040901:Proteomics; D051381:Rats; D017208:Rats, Wistar; D012149:Restraint, Physical; D013312:Stress, Physiological
chemical_list: D006360:Heat-Shock Proteins