title: Lectin microarray analysis of pluripotent and multipotent stem cells.
abstract: Stem cells have a capability to self-renew and differentiate into multiple types of cells; specific markers are available to identify particular stem cells for developmental biology research. In this study, we aimed to define the status of somatic stem cells and the pluripotency of human embryonic stem (hES) and induced pluripotent stem (iPS) cells using a novel molecular methodology, lectin microarray analysis. Our lectin microarray analysis successfully categorized murine somatic stem cells into the appropriate groups of differentiation potency. We then classified hES and iPS cells by the same approach. Undifferentiated hES cells were clearly distinguished from differentiated hES cells after embryoid formation. The pair-wise comparison means based on 'false discovery rate' revealed that three lectins -Euonymus europaeus lectin (EEL), Maackia amurensis lectin (MAL) and Phaseolus vulgaris leucoagglutinin [PHA(L)]- generated maximal values to define undifferentiated and differentiated hES cells. Furthermore, to define a pluripotent stem cell state, we generated a discriminant for the undifferentiated state with pluripotency. The discriminant function based on lectin reactivities was highly accurate for judgment of stem cell pluripotency. These results suggest that glycomic analysis of stem cells leads to a novel comprehensive approach for quality control in cell-based therapy and regenerative medicine.
authors.affiliation: Department of Reproductive Biology, National Institute for Child Health and Development, 2-10-1 Okura, Setagaya-ku, Tokyo 157-8535, Japan.
authors.forename: Masashi
authors.initials: M
authors.lastname: Toyoda
authors.forename: Mayu
authors.initials: M
authors.lastname: Yamazaki-Inoue
authors.forename: Yoko
authors.initials: Y
authors.lastname: Itakura
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kuno
authors.forename: Tomohisa
authors.initials: T
authors.lastname: Ogawa
authors.forename: Masao
authors.initials: M
authors.lastname: Yamada
authors.forename: Hidenori
authors.initials: H
authors.lastname: Akutsu
authors.forename: Yuji
authors.initials: Y
authors.lastname: Takahashi
authors.forename: Seiichi
authors.initials: S
authors.lastname: Kanzaki
authors.forename: Hisashi
authors.initials: H
authors.lastname: Narimatsu
authors.forename: Jun
authors.initials: J
authors.lastname: Hirabayashi
authors.forename: Akihiro
authors.initials: A
authors.lastname: Umezawa
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D002478:Cells, Cultured; D053595:Embryonic Stem Cells / Q000166:cytology; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology; D037102:Lectins; D059630:Mesenchymal Stem Cells; D051379:Mice; D046228:Microarray Analysis / Q000379:methods*; D039902:Multipotent Stem Cells / Q000378:metabolism; D039904:Pluripotent Stem Cells / Q000166:cytology; D011134:Polysaccharides; D011786:Quality Control
chemical_list: D037102:Lectins; D011134:Polysaccharides