title: Comprehensive multiresidue method for the simultaneous determination of 74 pesticides and metabolites in traditional Chinese herbal medicines by accelerated solvent extraction with high-performance liquid chromatography/tandem mass spectrometry.
abstract: In this paper, a multiresidue method for the simultaneous target analysis of 74 pesticides and metabolites in traditional Chinese herbal medicines (TCHMs) was developed using accelerated solvent extraction (ASE) coupled with HPLC/MS/MS. Pesticide residues were extracted from the different samples using ASE, then purified by gel permeation chromatography and graphitized carbon black/primary, secondary amine SPE. Gradient elution was used in conjunction with positive mode electrospray ionization MS/MS to detect 74 pesticides and metabolites from Cortex Cinnamomi, Flos Carthami, Folium Ginkgo, Herba Pogostemonis, Radix Ginseng, and Semen Ginkgo using a single chromatographic run. The analytical performance was demonstrated by the analysis of extracts spiked at three concentration levels ranging from 0.005 to 0.125 mg/kg for each pesticide and metabolite. In general, recoveries ranging from 70 to 110%, with RSDs better than 15%, were obtained. The recovery and repeatability data were in good accordance with European Union guidelines for pesticide residue analysis. The LOD for most of the targeted pesticides and metabolites tested was below 0.01 mg/kg.
authors.affiliation: Shanghai Institute for Food and Drug Control, Department of Traditional Chinese Medicine, No.1500 Zhangheng Rd, Shanghai 201203, People's Republic of China.
authors.forename: Zhengwei
authors.initials: Z
authors.lastname: Jia
authors.forename: Xiuhong
authors.initials: X
authors.lastname: Mao
authors.forename: Ke
authors.initials: K
authors.lastname: Chen
authors.forename: Ke
authors.initials: K
authors.lastname: Wang
authors.forename: Shen
authors.initials: S
authors.lastname: Ji
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004340:Drug Contamination; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D057230:Limit of Detection; D008516:Medicine, Chinese Traditional; D010573:Pesticide Residues / Q000032:analysis*; D052616:Solid Phase Extraction; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D004365:Drugs, Chinese Herbal; D010573:Pesticide Residues