title: Kinetics of filamentous phage assembly.
abstract: Filamentous phages release their progeny particles by a secretory process without lysing the bacterial cell. By this process about 6 viral particles per min are secreted from each cell. We show here that when the major coat protein (gp8) is provided from a plasmid we observe a phage progeny production rate depending on the induction of gp8 by IPTG. We also show that a transfection of Escherichia coli lacking F-pili is observed using a mutant of M13 that carries an ampicillin resistance gene, and phage particles are secreted in the absence of an F-plasmid. Extruding phage was visualized by atomic force microscopy (AFM) and by transmission electron microscopy (TEM) using gold-labeled antibodies to the major coat protein.
authors.affiliation: Institute of Microbiology and Molecular Biology, University of Hohenheim, 70599 Stuttgart, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Ploss
authors.forename: Andreas
authors.initials: A
authors.lastname: Kuhn
mesh_terms: D001435:Bacteriophages / Q000502:physiology* / Q000648:ultrastructure; D036022:Capsid Proteins / Q000502:physiology; D004926:Escherichia coli / Q000235:genetics; D007700:Kinetics; D018625:Microscopy, Atomic Force; D046529:Microscopy, Electron, Transmission; D010957:Plasmids; D014162:Transfection; D019065:Virus Assembly
chemical_list: D036022:Capsid Proteins