title: Generation of viable male and female mice from two fathers.
abstract: In sexual species, fertilization of oocytes produces individuals with alleles derived from both parents. Here we use pluripotent stem cells derived from somatic cells to combine the haploid genomes from two males to produce viable sons and daughters. Male (XY) mouse induced pluripotent stem cells (Father #1) were used to isolate subclones that had spontaneously lost the Y chromosome to become genetically female (XO). These male-derived XO stem cells were used to generate female chimeras that were bred with genetically distinct males (Father #2), yielding progeny possessing genetic information that was equally derived from both fathers. Thus, functional oocytes can be generated from male somatic cells after reprogramming and spontaneous sex reversal. These findings have novel implications for mammalian reproduction and assisted reproductive technology.
authors.affiliation: Department of Genetics, The University of Texas M.D. Anderson Cancer Center, 1515 Holcombe Blvd., Houston, TX 77030, USA.
authors.forename: Jian Min
authors.initials: JM
authors.lastname: Deng
authors.forename: Kei
authors.initials: K
authors.lastname: Satoh
authors.forename: Hongran
authors.initials: H
authors.lastname: Wang
authors.forename: Hao
authors.initials: H
authors.lastname: Chang
authors.forename: Zhaoping
authors.initials: Z
authors.lastname: Zhang
authors.forename: M David
authors.initials: MD
authors.lastname: Stewart
authors.forename: Austin J
authors.initials: AJ
authors.lastname: Cooney
authors.forename: Richard R
authors.initials: RR
authors.lastname: Behringer
mesh_terms: D000818:Animals; D002470:Cell Survival; D002478:Cells, Cultured; D002678:Chimera / Q000196:embryology* / Q000235:genetics; D019976:Cloning, Organism / Q000379:methods*; D004622:Embryo, Mammalian; D005220:Fathers; D005260:Female; D057026:Induced Pluripotent Stem Cells / Q000502:physiology / Q000637:transplantation; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D008954:Models, Biological; D053652:Nuclear Transfer Techniques; D012727:Sex Characteristics