title: Evaluation of photodynamic therapy (PDT) procedures using microfluidic system.
abstract: A hybrid PDMS/glass microfluidic system for evaluation of the efficiency of photodynamic therapy is presented. 5-aminolevulinic acid (ALA) was used as a precursor of photosensitizer. The geometry of the microdevice presented in this paper enables to test different concentrations of the photosensitizer in a single assay. The viability of the A549 cells was determined 24 h after PDT procedure (irradiation with light which induced a photosensitizer accumulated in carcinoma cells, λ=625 nm). The presented results confirmed the possibility to perform the photodynamic therapy process in vitro in microscale and the possibility to assess its effectiveness. Moreover, because two identical microstructures on a single chip were performed, the microchip can be used for examination simultaneously various cell lines (carcinoma and normal) or various photosensitizers.
authors.affiliation: Department of Microbioanalytics, Faculty of Chemistry, Warsaw University of Technology, Noakowskiego 3,00-664 Warsaw, Poland. ejedrych@ch.pw.edu.pl
authors.forename: Elzbieta
authors.initials: E
authors.lastname: Jedrych
authors.forename: Zuzanna
authors.initials: Z
authors.lastname: Pawlicka
authors.forename: Michal
authors.initials: M
authors.lastname: Chudy
authors.forename: Artur
authors.initials: A
authors.lastname: Dybko
authors.forename: Zbigniew
authors.initials: Z
authors.lastname: Brzozka
mesh_terms: D000622:Aminolevulinic Acid / Q000737:chemistry / Q000627:therapeutic use / Q000633:toxicity; D045744:Cell Line, Tumor; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D006801:Humans; D056656:Lab-On-A-Chip Devices; D008175:Lung Neoplasms / Q000188:drug therapy; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000737:chemistry / Q000627:therapeutic use / Q000633:toxicity; D011524:Protoporphyrins / Q000737:chemistry
chemical_list: D004129:Dimethylpolysiloxanes; D017319:Photosensitizing Agents; D011524:Protoporphyrins; C013830:baysilon; D000622:Aminolevulinic Acid; C028025:protoporphyrin IX