title: [Changes of the certain functions of mitochondria induced by acute exercise and the protective effect of bilirubin].
abstract: OBJECTIVE
  To study the mechanism of fatigue induced by acute exercise and the protective effect of bilirubin.
  
  
  METHODS
  41 Wistar male rats were divided into five groups: control group, exercise group, exercise recovery group, bilirubin-treated exercise group and bilirubin-treated exercise recovery group. The rats were administrated with 1 micromol/kg body weight of bilirubin or saline once every day for 4 weeks, after swimming with load for 2 h, all of the rats were killed and several index were determined.
  
  
  RESULTS
  Acute exercise could induce the increase of Ca2+ content in cytoplasm and mitochondria of gastrocnemius, Ca2+ content increased continuously after 12 hours in mitochondria. The Ca2(+)-Mg2(+)-ATPase activity decreased obviously after acute exercise, and the activity increased again after 12 hours. Bilirubin could inhibit these changes. The change of Mg2(+)-ATPase activity in mitochondria had little difference between bilirubin-treated and untreated groups, both of which were lower than that of control group, but the recovery was faster in the bilirubin-treated group than that in untreated group.
  
  
  CONCLUSIONS
  Bilirubin could protect muscle fibers from injury induced by acute exercise and delay the development of fatigue and promote the recovery through inhibiting the disturbance of the certain function of mitochondria.
authors.affiliation: Faculty of Preventive Medicine, Capitial University of Medical Science, Beijing 100054.
authors.forename: C H
authors.initials: CH
authors.lastname: Han
authors.forename: Q E
authors.initials: QE
authors.lastname: Wang
authors.forename: S
authors.initials: S
authors.lastname: Wang
authors.forename: H Q
authors.initials: HQ
authors.lastname: Shen
mesh_terms: D000818:Animals; D001663:Bilirubin / Q000502:physiology*; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D005221:Fatigue / Q000378:metabolism*; D008297:Male; D008928:Mitochondria / Q000502:physiology*; D010805:Physical Conditioning, Animal; D051381:Rats; D017208:Rats, Wistar
chemical_list: D017301:Ca(2+) Mg(2+)-ATPase; D001663:Bilirubin; D002118:Calcium