title: Label-free second-harmonic phase imaging of biological specimen by digital holographic microscopy.
abstract: We have previously developed a new way for nonscanning second-harmonic generation (SHG) microscopy [Opt. Lett. 34, 2450 (2009)]. Based on digital holography, this technique captures, in single-shot hologram acquisition, both the amplitude and the phase of a coherent SHG radiation, which makes possible second harmonic phase microscopy. In this work, we present holographic SHG phase microscopy of a label-free biological tissue and discuss its added value to SHG microscopy.
authors.affiliation: École Polytechnique Fédérale de Lausanne (EPFL), CH-1015 Lausanne, Switzerland. etienne.shaffer@epfl.ch
authors.forename: Etienne
authors.initials: E
authors.lastname: Shaffer
authors.forename: Corinne
authors.initials: C
authors.lastname: Moratal
authors.forename: Pierre
authors.initials: P
authors.lastname: Magistretti
authors.forename: Pierre
authors.initials: P
authors.lastname: Marquet
authors.forename: Christian
authors.initials: C
authors.lastname: Depeursinge
mesh_terms: D000818:Animals; D020405:Dermis / Q000166:cytology; D000078404:Epidermal Cells; D006696:Holography / Q000379:methods*; D007091:Image Processing, Computer-Assisted; D051379:Mice; D008853:Microscopy / Q000379:methods*; D057054:Molecular Imaging / Q000379:methods*