title: Effects of α-melanocyte-stimulating hormone on calcium concentration in SZ95 sebocytes.
abstract: Melanocortins have been implicated in human sebum secretion for a long time. However, the signalling pathways of α-melanocyte-stimulating hormone (α-MSH) in human sebocytes expressing melanocortin receptors (MC-Rs) are still poorly understood. Because calcium ions play a central role in MC-R signalling, we investigated whether α-MSH affects calcium signalling in the immortalized human sebocyte cell line SZ95. In addition, we investigated the impact of α-MSH on MC-1R expression and lipid synthesis in these cells. α-MSH increased intracellular calcium levels. α-MSH-mediated calcium mobilization originated from intracellular calcium stores and was mediated by inositol triphosphate. Moreover, α-MSH increased MC-1R immunoreactivity and lipid synthesis in SZ95 sebocytes in the presence of testosterone. Our data demonstrate that α-MSH in human sebocytes controls a key cellular signalling pathway, the calcium ion response, which may coordinate MC-1R-mediated sebum secretion.
authors.affiliation: Department of Dermatology, Yonsei University College of Medicine, Seoul, Korea.
authors.forename: Sung Won
authors.initials: SW
authors.lastname: Whang
authors.forename: Sang Eun
authors.initials: SE
authors.lastname: Lee
authors.forename: Ji Min
authors.initials: JM
authors.lastname: Kim
authors.forename: Hyun Jung
authors.initials: HJ
authors.lastname: Kim
authors.forename: Se Kyoo
authors.initials: SK
authors.lastname: Jeong
authors.forename: Christos C
authors.initials: CC
authors.lastname: Zouboulis
authors.forename: Jeong Taek
authors.initials: JT
authors.lastname: Seo
authors.forename: Seung Hun
authors.initials: SH
authors.lastname: Lee
mesh_terms: D020013:Calcium Signaling / Q000187:drug effects*; D002460:Cell Line; D006801:Humans; D008055:Lipids / Q000096:biosynthesis; D044102:Receptor, Melanocortin, Type 1 / Q000378:metabolism; D012627:Sebaceous Glands / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D012629:Sebum / Q000187:drug effects / Q000378:metabolism; D013739:Testosterone / Q000494:pharmacology; D000521:alpha-MSH / Q000494:pharmacology*
chemical_list: D008055:Lipids; D044102:Receptor, Melanocortin, Type 1; D013739:Testosterone; D000521:alpha-MSH