title: Fate and complex pathogenic effects of dioxins and polychlorinated biphenyls in obese subjects before and after drastic weight loss.
abstract: BACKGROUND
  In humans, persistent organic pollutants (POPs) are stored primarily in adipose tissue. Their total body burden and their contribution to obesity-associated diseases remain unclear.
  
  
  OBJECTIVE
  We characterized POP total body burden and their redistribution in obese individuals before and after drastic weight loss and compared these values with a variety of molecular, biological, and clinical parameters.
  
  
  METHODS
  Seventy-one obese subjects were enrolled and underwent bariatric surgery. Blood and adipose tissue samples were obtained at different times from these individuals as well as from 18 lean women.
  
  
  RESULTS
  POP content (17 dioxins/furans and 18 polychlorinated biphenyl congeners) in different adipose tissue territories was similar, allowing us to assess total POP body burden from a single biopsy. Total POP body burden was 2 to 3 times higher in obese than in lean individuals. We also found increased expression of some POP target genes in obese adipose tissue. Drastic weight loss led to increased serum POPs and, within 6-12 months, to a significant 15% decrease in total polychlorinated biphenyl body burden. Importantly, serum POP levels were positively correlated with liver toxicity markers and lipid parameters, independently of age and body mass index.
  
  
  CONCLUSIONS
  POP content in adipose tissue and serum correlate with biological markers of obesity-related dysfunctions. Drastic weight loss leads to a redistribution of POPs and to a moderate decrease of their total body burden.
authors.affiliation: INSERM, UMR-S, Université Paris Descartes, Centre Universitaire des Saints-Pères, France.
authors.forename: Min-Ji
authors.initials: MJ
authors.lastname: Kim
authors.forename: Philippe
authors.initials: P
authors.lastname: Marchand
authors.forename: Corneliu
authors.initials: C
authors.lastname: Henegar
authors.forename: Jean-Philippe
authors.initials: JP
authors.lastname: Antignac
authors.forename: Rohia
authors.initials: R
authors.lastname: Alili
authors.forename: Christine
authors.initials: C
authors.lastname: Poitou
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Bouillot
authors.forename: Arnaud
authors.initials: A
authors.lastname: Basdevant
authors.forename: Bruno
authors.initials: B
authors.lastname: Le Bizec
authors.forename: Robert
authors.initials: R
authors.lastname: Barouki
authors.forename: Karine
authors.initials: K
authors.lastname: Clément
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism; D000328:Adult; D001189:Aryl Hydrocarbon Hydroxylases / Q000235:genetics / Q000378:metabolism; D001822:Body Burden; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism*; D065633:Cytochrome P-450 CYP1B1; D004147:Dioxins / Q000378:metabolism / Q000633:toxicity*; D004785:Environmental Pollutants / Q000378:metabolism / Q000633:toxicity*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000235:genetics / Q000378:metabolism; D009765:Obesity / Q000097:blood / Q000378:metabolism* / Q000473:pathology; D011078:Polychlorinated Biphenyls / Q000378:metabolism / Q000633:toxicity*; D015431:Weight Loss
chemical_list: D004147:Dioxins; D004785:Environmental Pollutants; D011078:Polychlorinated Biphenyls; D001189:Aryl Hydrocarbon Hydroxylases; C585496:CYP1B1 protein, human; D019363:Cytochrome P-450 CYP1A1; D065633:Cytochrome P-450 CYP1B1; D016660:NAD(P)H Dehydrogenase (Quinone); C447479:NQO1 protein, human