title: Determination of ligustilide in the brains of freely moving rats using microdialysis coupled with ultra performance liquid chromatography/mass spectrometry.
abstract: In this paper, microdialysis combined with ultra performance liquid chromatography/mass spectrometry (UPLC-MS) was applied for the determination of ligustilide in freely moving rats' brain. The extracellular unbound ligustilide could be detected in rat brain within the first time interval (0-20 min) after nasal administration but not after oral administration. This result showed that ligustilide could quickly enter the brain after nasal administration; which implied that ligustilide may have a rapid onset of action. This work demonstrated the potential application of microdialysis combined with UPLC-MS in pharmacokinetic studies. Furthermore, the advantage that fewer animals used in the microdialysis experiment was confirmed.
authors.affiliation: Jiangsu Key laboratory for TCM Formulae Research, Nanjing University of Chinese Medicine, Nanjing, PR China.
authors.forename: Jianming
authors.initials: J
authors.lastname: Guo
authors.forename: Er-xin
authors.initials: EX
authors.lastname: Shang
authors.forename: Jin-ao
authors.initials: JA
authors.lastname: Duan
authors.forename: Yuping
authors.initials: Y
authors.lastname: Tang
authors.forename: Dawei
authors.initials: D
authors.lastname: Qian
mesh_terms: D015107:4-Butyrolactone / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000493:pharmacokinetics; D058956:Administration, Mucosal; D000818:Animals; D001921:Brain / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D030004:Ligusticum / Q000737:chemistry*; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D017551:Microdialysis / Q000379:methods; D009297:Nasal Mucosa; D010936:Plant Extracts / Q000008:administration & dosage / Q000493:pharmacokinetics*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D010936:Plant Extracts; C027820:ligustilide; D015107:4-Butyrolactone