title: Trans-acting RNAs as molecular probes for monitoring time-dependent structural change of an RNA complex adapting two structures.
abstract: As dynamic structural changes are pivotal for the functions of some classes of RNA molecule, it is important to develop methods to monitor structural changes in RNA in a time-dependent manner without chemical modification. Based on previous reports that trans-acting RNAs can be used as probes for analysis and control of 3D structures of target RNAs, we applied this method to monitor time-dependent structural changes in RNA. We designed and performed a proof-of-principle study using a simple model RNA complex that adopts two different structures as a target. The time-dependent structural changes in the target RNA were successfully monitored using two trans-acting RNAs, which stably form a ternary complex with the bimolecular target RNA and act as a catalyst to join two RNA fragments of the target complex, respectively.
authors.affiliation: Department of Chemistry and Biochemistry, Graduate School of Engineering, Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka 819-0395, Japan.
authors.forename: Yuri
authors.initials: Y
authors.lastname: Maeda
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Furuta
authors.forename: Yoshiya
authors.initials: Y
authors.lastname: Ikawa
mesh_terms: D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D015341:Nucleic Acid Probes / Q000737:chemistry*; D012313:RNA / Q000737:chemistry*; D016337:RNA, Catalytic / Q000737:chemistry; D013997:Time Factors
chemical_list: D015341:Nucleic Acid Probes; D016337:RNA, Catalytic; D012313:RNA