title: Arginine kinase is highly expressed in a resistant strain of silkworm (Bombyx mori, Lepidoptera): Implication of its role in resistance to Bombyx mori nucleopolyhedrovirus.
abstract: A gene encoding Bombyx mori arginine kinase (BmAK) has been indentified differentially expressed in the midguts of Bombyx mori strain NB which is resistant to nucleopolyhedrovirus (BmNPV), strain 306 which is susceptible to NPV and a near isogenic line BC(8) with similar genetic background to 306 but resistant to NPV by two-dimensional gel electrophoresis (2-DE). In this study, we characterized the expression profiles of BmAK using RT-PCR and real-time quantitative PCR. The expression level of BmAK fluctuated in various developing stage and various tissue. Remarkably, the expression level of BmAK increased more than 10-fold 24 hours post inoculation (h p.i.) of NPV in strain NB and BC(8), while such increment was abraded in strain 306 although the basal expression level of BmAK in strain 306 was higher than that of strain NB and BC(8). Western blotting analysis using polyclonal antibody against BmAK verified such observation, and immunofluoresence analysis indicated for the first time that BmAK was mainly located to the cytoplasm or some structures in cytoplasm. These findings suggest that arginine kinase is involved in the antiviral process of Bombyx mori larvae against NPV infection.
authors.affiliation: Institute of Life Sciences, Jiangsu University, Zhenjiang, Jiangsu 212013, P. R. China.
authors.forename: Lequn
authors.initials: L
authors.lastname: Kang
authors.forename: Haifeng
authors.initials: H
authors.lastname: Shi
authors.forename: Xiaoyong
authors.initials: X
authors.lastname: Liu
authors.forename: Chiyu
authors.initials: C
authors.lastname: Zhang
authors.forename: Qin
authors.initials: Q
authors.lastname: Yao
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.forename: Cheng
authors.initials: C
authors.lastname: Chang
authors.forename: Jing
authors.initials: J
authors.lastname: Shi
authors.forename: Jian
authors.initials: J
authors.lastname: Cao
authors.forename: Jie
authors.initials: J
authors.lastname: Kong
authors.forename: Keping
authors.initials: K
authors.lastname: Chen
mesh_terms: D000818:Animals; D001122:Arginine Kinase / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D015153:Blotting, Western; D012831:Bombyx / Q000201:enzymology* / Q000254:growth & development / Q000821:virology*; D017924:Nucleopolyhedroviruses; D016133:Polymerase Chain Reaction; D014018:Tissue Distribution
chemical_list: D001122:Arginine Kinase