title: Viability and apoptosis in spermatozoa of transgenic rabbits.
abstract: The aim of our study was to compare the viability of sperm cells from transgenic (mWAP-hFVIII gene) or non-transgenic (normal) rabbit males as assessed by viability (SYBR-14/PI) and apoptosis (annexin V) tests. These results were evaluated using female conception rates following insemination with the respective sperm samples. No significant differences were found in concentration and motility between transgenic and non-transgenic spermatozoa. Spermatozoa from both transgenic (63.05 ± 20.05%) or non-transgenic (65.75 ± 22.15%) males, stained with SYBR-14 (green), were found to be morphologically normal. In both groups, the highest proportion of annexin V-positive sperm staining was found in the post-acrosomal part of the sperm head (8.66 and 27.53%). The percentage of sperm that stained with SYBR-14/PI or with annexin V/DAPI was correlated with liveborn in transgenic rabbits (R2 = 0.6118 and R2 = 0.2187, respectively) or non-transgenic rabbits (R2 = 0.671 and R2 = 0.3579, respectively). These data indicate that there was no difference in the viability of rabbit transgenic and non-transgenic spermatozoa when determined by both fluorescence assays.
authors.affiliation: Animal Production Research Centre Nitra, 95141 Luzianky near Nitra, Slovak Republic. chrenekp@yahoo.com
authors.forename: P
authors.initials: P
authors.lastname: Chrenek
authors.forename: A V
authors.initials: AV
authors.lastname: Makarevich
authors.forename: M
authors.initials: M
authors.lastname: Simon
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified / Q000378:metabolism / Q000502:physiology*; D017304:Annexin A5 / Q000378:metabolism; D017209:Apoptosis; D002470:Cell Survival; D005260:Female; D007315:Insemination, Artificial / Q000662:veterinary; D050498:Live Birth / Q000235:genetics / Q000662:veterinary; D008297:Male; D009930:Organic Chemicals / Q000378:metabolism; D011247:Pregnancy; D011817:Rabbits / Q000502:physiology*; D013076:Sperm Count; D013081:Sperm Motility; D013094:Spermatozoa / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D013194:Staining and Labeling / Q000379:methods
chemical_list: D017304:Annexin A5; D009930:Organic Chemicals; C093207:SYBR-14