title: Structure analysis of group I plant nucleases.
abstract: Anticancer drugs attacking nucleic acids of the target cells have so far been based on animal or fungal ribonucleases. Plant nucleases have been proved to exhibit decreased cytotoxic side effects. Tomato bifunctional nuclease 1 with activity against both single-stranded and double-stranded RNA and DNA was produced in tobacco leaves as recombinant protein. The enzyme crystallizes under several different crystallization conditions. The presence of Zn(2+) ions was confirmed by X-ray fluorescence. First crystallographic data were obtained.
authors.affiliation: Institute of Macromolecular Chemistry AS CR, v.v.i., Heyrovského nám. 2, 162 06 Praha 6, Czech Republic. dohnalek007@gmail.com
authors.forename: Jan
authors.initials: J
authors.lastname: Dohnálek
authors.forename: Tomáš
authors.initials: T
authors.lastname: Koval'
authors.forename: Petra
authors.initials: P
authors.lastname: Lipovová
authors.forename: Tomáš
authors.initials: T
authors.lastname: Podzimek
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Matoušek
mesh_terms: D004277:DNA, Single-Stranded / Q000378:metabolism; D004706:Endodeoxyribonucleases / Q000737:chemistry*; D004722:Endoribonucleases / Q000737:chemistry*; D018551:Solanum lycopersicum / Q000201:enzymology*; D012330:RNA, Double-Stranded / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry; D014026:Nicotiana / Q000201:enzymology; D014961:X-Ray Diffraction; D015032:Zinc / Q000032:analysis
chemical_list: D004277:DNA, Single-Stranded; D012330:RNA, Double-Stranded; D011994:Recombinant Proteins; D004706:Endodeoxyribonucleases; D004722:Endoribonucleases; D015032:Zinc