title: Insufficiency of the Kanagawa hemolytic test for detecting pathogenic Vibrio parahaemolyticus in Shanghai, China.
abstract: We evaluated the Kanagawa hemolytic test and tdh gene test for accuracy in identifying pathogenic Vibrio parahaemolyticus isolates in Shanghai. One hundred and seventy-two V. parahaemolyticus isolates were collected from diarrhea patients, freshly harvested sea fish, or fresh water samples. Statistical data for the Kanagawa hemolytic test and tdh gene test were compared. There were 83.51% isolates (81/97) from patients and 22.22% isolates (10/45) from sea-fish positive for the tdh gene. However, none of 30 isolates from fresh water samples were tdh-positive. Positive Kanagawa hemolytic tests were obtained in 88.66%, 46.67%, and 76.67% of isolates, which were from patients, sea fish, and fresh water samples, respectively. Positive rates of the Kanagawa hemolytic tests and the tdh gene tests were significantly different in isolates from those 3 sources (P < 0.001). The tdh gene test showed higher specificity than the Kanagawa hemolytic test on identifying pathogenic V. parahaemolyticus isolates in Shanghai, China.
authors.affiliation: Department of Epidemiology, Shanghai Public Health Clinical Center, Fudan University affiliated, Shanghai, P.R. China.
authors.forename: Wang
authors.initials: W
authors.lastname: Hongping
authors.forename: Zhang
authors.initials: Z
authors.lastname: Jilun
authors.forename: Jiang
authors.initials: J
authors.lastname: Ting
authors.forename: Bao
authors.initials: B
authors.lastname: Yixi
authors.forename: Zhou
authors.initials: Z
authors.lastname: Xiaoming
mesh_terms: D000818:Animals; D001427:Bacterial Toxins / Q000235:genetics / Q000378:metabolism; D001431:Bacteriological Techniques / Q000379:methods*; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005399:Fishes / Q000382:microbiology*; D006460:Hemolysin Proteins / Q000235:genetics* / Q000378:metabolism*; D006461:Hemolysis; D006801:Humans; D012680:Sensitivity and Specificity; D014735:Vibrio Infections / Q000175:diagnosis*; D014736:Vibrio parahaemolyticus / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D014871:Water Microbiology
chemical_list: D001427:Bacterial Toxins; D004269:DNA, Bacterial; D006460:Hemolysin Proteins; C068489:thermostable direct hemolysin