title: E6 and E7 proteins from different beta-papillomaviruses types do not interfere in UVB-induced apoptosis of HaCaT keratinocytes.
abstract: Beta-papillomaviruses (beta-HPV) have been linked to the development of skin cancer in humans. Because both E6 and E7 proteins from beta-HPV have been involved in the potential carcinogenicity of these viruses, we investigated their role on UVB-induced apoptosis in HaCaT cell line. HaCaT cells have been transduced with both E6/E7 using a retroviral system and treated with PRIMA-1. Apoptosis was assessed by flow cytometry to measure mitochondrial membrane potential and DNA fragmentation. HaCat keratinocytes transduced with both E6 and E7 genes of seven beta-HPV types (HPV5, HPV8, HPV14, HPV24, HPV36, HPV38 and HPV49) did not demonstrate any inhibition of UVB-induced apoptosis, even after p53 reactivation through PRIMA-1. Our data suggest that the expression of E6 and E7 exert different modulatory effects on UVB-induced apoptosis according to beta-HPV types and to the cellular genetic context.
authors.affiliation: EA 3181, IFR 133, Université de Franche-Comté, Besançon, France.
authors.forename: Jean-Sébastien
authors.initials: JS
authors.lastname: Guerrini
authors.forename: Véronique
authors.initials: V
authors.lastname: Bouvard
authors.forename: Evelyne
authors.initials: E
authors.lastname: Oswald
authors.forename: Angel
authors.initials: A
authors.lastname: Alonso
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Prétet
authors.forename: Massimo
authors.initials: M
authors.lastname: Tommasino
authors.forename: Christiane
authors.initials: C
authors.lastname: Mougin
authors.forename: François
authors.initials: F
authors.lastname: Aubin
mesh_terms: D017209:Apoptosis / Q000187:drug effects / Q000528:radiation effects*; D001372:Aza Compounds / Q000494:pharmacology; D052686:Betapapillomavirus / Q000472:pathogenicity* / Q000502:physiology; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000494:pharmacology; D002460:Cell Line; D003043:Cocarcinogenesis; D006801:Humans; D015603:Keratinocytes / Q000473:pathology / Q000528:radiation effects* / Q000821:virology*; D009856:Oncogene Proteins, Viral / Q000502:physiology*; D012878:Skin Neoplasms / Q000209:etiology; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000502:physiology; D014466:Ultraviolet Rays / Q000009:adverse effects*
chemical_list: D001372:Aza Compounds; D019086:Bridged Bicyclo Compounds, Heterocyclic; D009856:Oncogene Proteins, Viral; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; C451219:2,2-bis(hydroxymethyl)-1-azabicyclo(2,2,2,)octan-3-one