title: Coexpression of an intronic microRNA and its host gene reveals a potential role for miR-483-5p as an IGF2 partner.
abstract: MicroRNAs (miRNAs) are endogenous noncoding RNAs that downregulate gene expression by inhibiting translation or promoting mRNA degradation. Although over one-third of miRNAs are located within the intronic regions of transcription units, the expression of such "intron-derived" (or "intronic") miRNAs and their relationship with their host gene remain a mystery. Here, we show that miR-483-5p, which is located within the second intron of the insulin-like growth factor (Igf2) gene, is downregulated in mouse Hepa1-6 cells in a direct correlation with the Igf2 transcript by chromeceptin, an inhibitor of Igf2 at the transcriptional level. Furthermore, miR-483-5p overexpression and knockdown regulates the suppressor of cytokine signalling 3 (Socs3) and Igf2 mRNAs, respectively. Finally, Socs3, a key putative leptin-resistant factor in obesity, is identified as a direct target of miR-483-5p. These data suggest that miR-483-5p can be coexpressed together with its host gene, Igf2, and revealed the link between this miRNA and the IGF2 growth factor. In addition, these observations not only provide supporting evidence for the codependent expression of intronic miRNAs and their host genes in vitro, but also give insight into the role of miR-483-5p in metabolism regulation and tumourigenesis.
authors.affiliation: Harbin Medical University, Harbin, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Ma
authors.forename: Xidi
authors.initials: X
authors.lastname: Wang
authors.forename: Yu
authors.initials: Y
authors.lastname: Qiao
authors.forename: Fuyuan
authors.initials: F
authors.lastname: Li
authors.forename: Yang
authors.initials: Y
authors.lastname: Hui
authors.forename: Chaoxia
authors.initials: C
authors.lastname: Zou
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Jin
authors.forename: Guixiang
authors.initials: G
authors.lastname: Lv
authors.forename: Yahui
authors.initials: Y
authors.lastname: Peng
authors.forename: Lujing
authors.initials: L
authors.lastname: Wang
authors.forename: Hui
authors.initials: H
authors.lastname: Huang
authors.forename: Lingyun
authors.initials: L
authors.lastname: Zhou
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Zheng
authors.forename: Xu
authors.initials: X
authors.lastname: Gao
mesh_terms: D000818:Animals; D001578:Benzopyrans / Q000494:pharmacology; D045744:Cell Line, Tumor; D015870:Gene Expression; D005786:Gene Expression Regulation; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D007335:Insulin-Like Growth Factor II / Q000235:genetics*; D007438:Introns; D051379:Mice; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000235:genetics*; D009369:Neoplasms / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000071223:Suppressor of Cytokine Signaling 3 Protein; D050826:Suppressor of Cytokine Signaling Proteins / Q000235:genetics / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
chemical_list: D001578:Benzopyrans; C513121:IGF2 protein, mouse; D035683:MicroRNAs; C548913:Mirn483 microRNA, mouse; C491411:Socs3 protein, mouse; D000071223:Suppressor of Cytokine Signaling 3 Protein; D050826:Suppressor of Cytokine Signaling Proteins; D014409:Tumor Necrosis Factor-alpha; C560188:chromeceptin; D007335:Insulin-Like Growth Factor II