title: [Losartan's effects on the protein expression of the kidney of spontaneous hypertension rat].
abstract: OBJECTIVE
  To analyse the alterations of protein expression in the kidney of spontaneously hypertensive rat with losartan.
  
  
  METHODS
  The proteins of the kidney were isolated by two-dimensional gel electrophoresis. The protein spots with significant changes were selected for further identification by LC-MS/MS.
  
  
  RESULTS
  The number of average protein spots of two groups was 570 +/- 48 and 686 +/- 30 respectively. Compared with the SHR, 13 spots had changed significantly after treated with losartan. There were 5 protein spots detected only in SHR group, while 4 up-regulated and 4 down-regulated protein spots were detected in SHR-L group. These differentially expressed proteins were detected by mass spectrometry. 7 spots were identified. There were Heat shock protein (Hsp), Tubulin alpha-1 chain, Transthyretin precursor, Liver regeneration-related protein LRRG03, Ezrin-radixin-moesin binding phosphoprotein 50, Phosphoglycerate kinase 1 and Anionic trypsin I precursor.
  
  
  CONCLUSIONS
  The different protein spots expression may play important roles in Losartan's effective protection to hypertension rats renal tissue.
authors.affiliation: Department of Cardiology, Taizhou Hospital, Linhai, 317000, China. dyx5113510@yahoo.com.cn
authors.forename: Yu-xi
authors.initials: YX
authors.lastname: Du
authors.forename: Min
authors.initials: M
authors.lastname: Yu
authors.forename: Jun-zhu
authors.initials: JZ
authors.lastname: Chen
authors.forename: Xing-xiang
authors.initials: XX
authors.lastname: Wang
authors.forename: Shu-ping
authors.initials: SP
authors.lastname: Li
mesh_terms: D047228:Angiotensin II Type 1 Receptor Blockers / Q000494:pharmacology / Q000627:therapeutic use; D000818:Animals; D002853:Chromatography, Liquid; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D006973:Hypertension / Q000188:drug therapy / Q000378:metabolism* / Q000503:physiopathology; D007668:Kidney / Q000378:metabolism* / Q000503:physiopathology; D019808:Losartan / Q000494:pharmacology* / Q000627:therapeutic use; D008297:Male; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D051381:Rats; D011918:Rats, Inbred SHR; D053719:Tandem Mass Spectrometry
chemical_list: D047228:Angiotensin II Type 1 Receptor Blockers; D020543:Proteome; D019808:Losartan