title: Natural and engineered hydroxyectoine production based on the Pseudomonas stutzeri ectABCD-ask gene cluster.
abstract: We report on the presence of a functional hydroxyectoine biosynthesis gene cluster, ectABCD-ask, in Pseudomonas stutzeri DSM5190(T) and evaluate the suitability of P. stutzeri DSM5190(T) for hydroxyectoine production. Furthermore, we present information on heterologous de novo production of the compatible solute hydroxyectoine in Escherichia coli. In this host, the P. stutzeri gene cluster remained under the control of its salt-induced native promoters. We also noted the absence of trehalose when hydroxyectoine genes were expressed, as well as a remarkable inhibitory effect of externally applied betaine on hydroxyectoine synthesis. The specific heterologous production rate in E. coli under the conditions employed exceeded that of the natural producer Pseudomonas stutzeri and, for the first time, enabled effective hydroxyectoine production at low salinity (2%), with the added advantage of simple product processing due to the absence of other cosolutes.
authors.affiliation: Institut für Mikrobiologie & Biotechnologie, Rheinische Friedrich-Wilhelms-Universität Bonn, Meckenheimer Allee 168, 53115 Bonn, Germany.
authors.forename: Britta
authors.initials: B
authors.lastname: Seip
authors.forename: Erwin A
authors.initials: EA
authors.lastname: Galinski
authors.forename: Matthias
authors.initials: M
authors.lastname: Kurz
mesh_terms: D000599:Amino Acids, Diamino / Q000096:biosynthesis* / Q000235:genetics*; D001622:Betaine / Q000378:metabolism / Q000494:pharmacology; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D007218:Industrial Microbiology; D008969:Molecular Sequence Data; D005810:Multigene Family; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D044223:Pseudomonas stutzeri / Q000235:genetics* / Q000378:metabolism*; D054712:Salinity; D014199:Trehalose / Q000378:metabolism
chemical_list: D000599:Amino Acids, Diamino; C121557:hydroxyectoine; D001622:Betaine; D014199:Trehalose