title: The actinobacterial signature protein ParJ (SCO1662) regulates ParA polymerization and affects chromosome segregation and cell division during Streptomyces sporulation.
abstract: Bacterial chromosome segregation usually involves cytoskeletal ParA proteins, ATPases which can form dynamic filaments. In aerial hyphae of the mycelial bacterium Streptomyces coelicolor, ParA filaments extend over tens of microns and are responsible for segregation of dozens of chromosomes. We have identified a novel interaction partner of S. coelicolor ParA, ParJ. ParJ negatively regulates ParA polymerization in vitro and is important for efficient chromosome segregation in sporulating aerial hyphae. ParJ-EGFP formed foci along aerial hyphae even in the absence of ParA. ParJ, which is encoded by sco1662, turned out to be one of the five actinobacterial signature proteins, and another of the five is a ParJ paralogue. We hypothesize that polar growth, which is characteristic not only of streptomycetes, but even of simple Actinobacteria, may be interlinked with ParA polymer assembly and its specific regulation by ParJ.
authors.affiliation: Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland.
authors.forename: Bartosz
authors.initials: B
authors.lastname: Ditkowski
authors.forename: Paulina
authors.initials: P
authors.lastname: Troć
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Ginda
authors.forename: Magdalena
authors.initials: M
authors.lastname: Donczew
authors.forename: Keith F
authors.initials: KF
authors.lastname: Chater
authors.forename: Jolanta
authors.initials: J
authors.lastname: Zakrzewska-Czerwińska
authors.forename: Dagmara
authors.initials: D
authors.lastname: Jakimowicz
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002455:Cell Division; D020090:Chromosome Segregation; D002876:Chromosomes, Bacterial / Q000235:genetics* / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D058105:Polymerization; D013171:Spores, Bacterial / Q000166:cytology / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D048372:Streptomyces coelicolor / Q000737:chemistry / Q000166:cytology / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; C052883:chromosome partition proteins, bacterial