title: [Dynamic changes of Smac/DIABLO protein expression in rat donor myocardium suffered from hypothermic preservation].
abstract: OBJECTIVE
  To investigate the effect of different duration of hypothermic preservation on the expression of Smac/DIABLO protein in rat hearts.
  
  
  METHODS
  The Langendorff model of isolated rat heart was used. After stored in 4 degrees C Celsior solution for different time (0, 3, 6, 9 or 12 h), the activity of SOD and the content of MDA in heart mitochondria were measured. Cell apoptosis was detected by TUNEL technique. The expression of Smac/DIABLO protein was also analyzed by Western blotting.
  
  
  RESULTS
  (1) After hypothermic preservation, the activity of SOD was decreased and the content of MDA was increased in rat hearts in a time-dependent manner. (2) Prolonged the hypothermic preservation, the percentage of apoptotic cell also enhanced. (3) After long-term of cold preservation, the expression of Smac/DIABLO protein increased at 3-6 h of preservation but decreased after 9 h.
  
  
  CONCLUSIONS
  Prolonged the hypothermic preservation might lead to the expression of Smac/DIABLO protein and induce cardiomyocytes apoptosis, which may in turn result in malfunction of cardiomyocytes.
authors.affiliation: Zhejiang Medical College, Hangzhou 310053, China.
authors.forename: Ming-zhi
authors.initials: MZ
authors.lastname: Zheng
authors.forename: Jian-ping
authors.initials: JP
authors.lastname: Jiang
authors.forename: Lin
authors.initials: L
authors.lastname: Fu
authors.forename: Li
authors.initials: L
authors.lastname: Zhu
authors.forename: Ying-ying
authors.initials: YY
authors.lastname: Chen
authors.forename: Yue-liang
authors.initials: YL
authors.lastname: Shen
mesh_terms: D000818:Animals; D051017:Apoptosis Regulatory Proteins; D002352:Carrier Proteins / Q000378:metabolism*; D003080:Cold Temperature; D006321:Heart; D016027:Heart Transplantation; D008297:Male; D024101:Mitochondrial Proteins / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism*; D009926:Organ Preservation / Q000379:methods*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D051017:Apoptosis Regulatory Proteins; D002352:Carrier Proteins; C489013:DIABLO protein, rat; D024101:Mitochondrial Proteins