title: Use of superoxide dismutase and catalase producing lactic acid bacteria in TNBS induced Crohn's disease in mice.
abstract: Reactive oxygen species are involved in various aspects of intestinal inflammation and tumor development. Decreasing their levels using antioxidant enzymes, such as catalase (CAT) or superoxide dismutase (SOD) could therefore be useful in the prevention of certain diseases. Lactic acid bacteria (LAB) are ideal candidates to deliver these enzymes in the gut. In this study, the anti-inflammatory effects of CAT or SOD producing LAB were evaluated using a trinitrobenzenesulfonic acid (TNBS) induced Crohn's disease murine model. Engineered Lactobacillus casei BL23 strains producing either CAT or SOD, or the native strain were given to mice before and after intrarectal administration of TNBS. Animal survival, live weight, intestinal morphology and histology, enzymatic activities, microbial translocation to the liver and cytokines released in the intestinal fluid were evaluated. The mice that received CAT or SOD-producing LAB showed a faster recovery of initial weight loss, increased enzymatic activities in the gut and lesser extent of intestinal inflammation compared to animals that received the wild-type strain or those that did not receive bacterial supplementation. Our findings suggest that genetically engineered LAB that produce antioxidant enzymes could be used to prevent or decrease the severity of certain intestinal pathologies.
authors.affiliation: Centro de Referencia para Lactobacilos (CERELA-CONICET), Chacabuco 145, San Miguel de Tucumán, Tucumán T4000ILC, Argentina. leblanc@cerela.org.ar
authors.forename: Jean Guy
authors.initials: JG
authors.lastname: LeBlanc
authors.forename: Silvina
authors.initials: S
authors.lastname: del Carmen
authors.forename: Anderson
authors.initials: A
authors.lastname: Miyoshi
authors.forename: Vasco
authors.initials: V
authors.lastname: Azevedo
authors.forename: Fernando
authors.initials: F
authors.lastname: Sesma
authors.forename: Philippe
authors.initials: P
authors.lastname: Langella
authors.forename: Luis G
authors.initials: LG
authors.lastname: Bermúdez-Humarán
authors.forename: Laurie
authors.initials: L
authors.lastname: Watterlot
authors.forename: Gabriela
authors.initials: G
authors.lastname: Perdigon
authors.forename: Alejandra
authors.initials: A
authors.lastname: de Moreno de LeBlanc
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002374:Catalase / Q000096:biosynthesis / Q000378:metabolism*; D003106:Colon / Q000473:pathology; D003424:Crohn Disease / Q000139:chemically induced / Q000382:microbiology / Q000517:prevention & control*; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D005818:Genetic Engineering; D006651:Histocytochemistry; D007249:Inflammation; D007420:Intestine, Large; D007780:Lacticaseibacillus casei / Q000201:enzymology* / Q000378:metabolism; D008099:Liver / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D019936:Probiotics / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000096:biosynthesis / Q000378:metabolism*; D014302:Trinitrobenzenesulfonic Acid
chemical_list: D016207:Cytokines; D014302:Trinitrobenzenesulfonic Acid; D002374:Catalase; D013482:Superoxide Dismutase