title: Upf1 ATPase-dependent mRNP disassembly is required for completion of nonsense- mediated mRNA decay.
abstract: Cellular mRNAs exist in messenger ribonucleoprotein (mRNP) complexes, which undergo transitions during the lifetime of the mRNAs and direct posttranscriptional gene regulation. A final posttranscriptional step in gene expression is the turnover of the mRNP, which involves degradation of the mRNA and recycling of associated proteins. How tightly associated protein components are released from degrading mRNPs is unknown. Here, we demonstrate that the ATPase activity of the RNA helicase Upf1 allows disassembly of mRNPs undergoing nonsense-mediated mRNA decay (NMD). In the absence of Upf1 ATPase activity, partially degraded NMD mRNA intermediates accumulate in complex with NMD factors and concentrate in processing bodies. Thus, disassembly and completion of turnover of mRNPs undergoing NMD requires ATP hydrolysis by Upf1. This uncovers a previously unappreciated and potentially regulated step in mRNA decay and raises the question of how other mRNA decay pathways release protein components of substrate mRNPs.
authors.affiliation: University of Colorado, Boulder, 80309, USA.
authors.forename: Tobias M
authors.initials: TM
authors.lastname: Franks
authors.forename: Guramrit
authors.initials: G
authors.lastname: Singh
authors.forename: Jens
authors.initials: J
authors.lastname: Lykke-Andersen
mesh_terms: D002460:Cell Line; D018389:Codon, Nonsense; D006367:HeLa Cells; D006801:Humans; D020365:RNA Helicases; D020871:RNA Stability; D012261:Ribonucleoproteins / Q000378:metabolism*; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*
chemical_list: D018389:Codon, Nonsense; D012261:Ribonucleoproteins; D015534:Trans-Activators; C026452:messenger ribonucleoprotein; D020365:RNA Helicases; C101828:UPF1 protein, human