title: Expression of humanized anti-Her2/neu single-chain IgG1-like antibody in mammary glands of transgenic mice.
abstract: A system for production of single-chain antibody in mammary glands of mice was developed on the basis of a hybrid gene constructed from the coding sequence of anti-Her2/neu single-chain antibody inserted into the first exon of the sheep beta-lactoglobulin gene. Lines of transgenic mice were obtained that expressed humanized single-chain anti-Her2/neu IgG1-like antibody in their milk. These antibodies interact with Her2/neu antigen with high affinity (K(d) = 0.4 nM). The expression level of the transgene depended on its integration site in the genome but not on the copy number. The transgene had no toxic effect on the mice and was stably inherited, at least for two generations. The results reveal new opportunities of producing single-chain antibodies in the milk of animals.
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya street 16/10, Moscow, Russia.
authors.forename: Veronika
authors.initials: V
authors.lastname: Yuskevich
authors.forename: Yuriy
authors.initials: Y
authors.lastname: Khodarovich
authors.forename: German
authors.initials: G
authors.lastname: Kagarliskiy
authors.forename: Oleg
authors.initials: O
authors.lastname: Stremovskiy
authors.forename: Oksana
authors.initials: O
authors.lastname: Maksimenko
authors.forename: Sergey
authors.initials: S
authors.lastname: Lukash
authors.forename: Oleg
authors.initials: O
authors.lastname: Polanovsky
authors.forename: Sergey
authors.initials: S
authors.lastname: Deyev
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D005091:Exons / Q000235:genetics; D005260:Female; D015870:Gene Expression; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology*; D007782:Lactoglobulins / Q000235:genetics; D008297:Male; D008321:Mammary Glands, Animal / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D015202:Protein Engineering / Q000379:methods*; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000276:immunology*; D057127:Single-Chain Antibodies / Q000737:chemistry / Q000235:genetics* / Q000276:immunology*
chemical_list: D007074:Immunoglobulin G; D007782:Lactoglobulins; D018719:Erb-b2 Receptor Tyrosine Kinases; D057127:Single-Chain Antibodies