title: Double-stranded RNA adenosine deaminase ADAR-1-induced hypermutated genomes among inactivated seasonal influenza and live attenuated measles virus vaccines.
abstract: We sought to examine ADAR-1 editing of measles and influenza virus genomes derived from inactivated seasonal influenza and live attenuated measles virus vaccines grown on chicken cells as the culture substrate. Using highly sensitive 3DI-PCR (R. Suspène et al., Nucleic Acids Res. 36:e72, 2008), it was possible to show that ADAR-1 could hyperdeaminate adenosine residues in both measles virus and influenza virus A genomes. Detailed analysis of the dinucleotide editing context showed preferences for 5'ArA and 5'UrA, which is typical of editing in mammalian cells. The hyperedited mutant frequency, including genomes and antigenomes, was a log greater for influenza virus compared to measles virus, suggesting a greater sensitivity to restriction by ADAR-1.
authors.affiliation: Molecular Retrovirology Unit, Institut Pasteur, Paris Cedex 15, France.
authors.forename: Rodolphe
authors.initials: R
authors.lastname: Suspène
authors.forename: Vincent
authors.initials: V
authors.lastname: Petit
authors.forename: David
authors.initials: D
authors.lastname: Puyraimond-Zemmour
authors.forename: Marie-Ming
authors.initials: MM
authors.lastname: Aynaud
authors.forename: Michel
authors.initials: M
authors.lastname: Henry
authors.forename: Denise
authors.initials: D
authors.lastname: Guétard
authors.forename: Christophe
authors.initials: C
authors.lastname: Rusniok
authors.forename: Simon
authors.initials: S
authors.lastname: Wain-Hobson
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Vartanian
mesh_terms: D000243:Adenosine Deaminase / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002522:Chlorocebus aethiops; D016679:Genome, Viral; D006801:Humans; D009980:Influenza A virus / Q000235:genetics*; D007252:Influenza Vaccines / Q000235:genetics; D007251:Influenza, Human / Q000201:enzymology* / Q000821:virology; D008457:Measles / Q000201:enzymology* / Q000821:virology; D008458:Measles Vaccine / Q000235:genetics; D008459:Measles virus / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D016601:RNA-Binding Proteins; D012621:Seasons; D014613:Vaccines, Attenuated / Q000235:genetics; D014709:Vero Cells
chemical_list: D007252:Influenza Vaccines; D008458:Measles Vaccine; D016601:RNA-Binding Proteins; D014613:Vaccines, Attenuated; C567955:ADARB1 protein, human; D000243:Adenosine Deaminase