title: [The effects of neuropeptides on the regulations of gastric mucosal blood flow in central nervous system and periphery in rats].
abstract: OBJECTIVE
  To investigate the effects of calcitonin gene-related peptide (CGRP), gastrin 17 (G17), bombesin (Bom), met-enkephalin (Met-enk), neuropeptide Y (NPY) and somatostatin (SS) on GMBF and the role of endogenous NO in increased GMBF induced by neuropeptides in rats.
  
  
  METHODS
  By hydrogen gas clearance technique to measure gastric mucosal blood flow (GMBF) and arterial infusion close to stomach or intracerebroventricular (icv) to microinject neuropeptides.
  
  
  RESULTS
  (1) Arterial infusions of CGRP and G17 (5, 50 and 100 pmol x min(-1)) increased GMBF significantly in dose-dependent manners. CGRP had more effective effect on increasing GMBF than that of G17. Intravenous pretreatment of L-nitro-L-arginine methyl ester (L-NAME) to inhibit the synthesis of endogenous NO could abolish completely or partially the increases in GMBF response to CGRP or G17 respectively. (2) Arterial infusions of Bom and Met-enk (50 and 100 pmol x min(-1)) increased GMBF significantly. The increases in GMBF induced by Bom or Met-enk were abolished completely or partially by pretreatment of L-NAME respectively. (3) Arterial infusion of NPY (5, 50 and 100 pmol x min(-1)) led to reduction of GMBF significantly in a dose-dependent manner. SS (50 and 100 pmol x min(-1)) also reduced GMBF significantly. (4) icv microinjection of CGRP (10 microg) and G17 (10 Microg) increased GMBF significantly. The increases in GMBF induced by icv microinjection of CGRP or G17 were blocked completely or partially respectively by pretreatments with L-NAME. (5) icv microinjection of NPY (10 microg) decreased GMBF significantly.
  
  
  CONCLUSIONS
  Neuropeptides play important roles in the regulation of GMBF in rats and NO is involved in the increase of GMBF induced by some neuropeptides.
authors.affiliation: Department of Physiology, Nanjing Medical University, Nangjing 210029, China.
authors.forename: Luo
authors.initials: L
authors.lastname: Gu
authors.forename: Hong-Li
authors.initials: HL
authors.lastname: Xu
authors.forename: Chang-Dong
authors.initials: CD
authors.lastname: Yan
authors.forename: Su-Ping
authors.initials: SP
authors.lastname: Tian
authors.forename: Jun
authors.initials: J
authors.lastname: Du
authors.forename: Guang
authors.initials: G
authors.lastname: Chen
authors.forename: Ying-Bin
authors.initials: YB
authors.lastname: Ge
authors.forename: Dong-Sheng
authors.initials: DS
authors.lastname: Li
mesh_terms: D000818:Animals; D005753:Gastric Mucosa / Q000098:blood supply* / Q000187:drug effects*; D008297:Male; D009479:Neuropeptides / Q000494:pharmacology* / Q000502:physiology; D009569:Nitric Oxide / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D009479:Neuropeptides; D009569:Nitric Oxide