title: Cellular expression of the K+-Cl- cotransporter KCC3 in the central nervous system of mouse.
abstract: Potassium/Chloride cotransporters are transmembrane proteins that regulate cell volume and control neuronal activity by transporting K(+) and Cl(-) ions across the plasma membrane. Potassium/Chloride cotransporter 3 (KCC3) mutations are responsible for hereditary motor and sensory neuropathy with agenesis of the corpus callosum (HMSN/ACC), which is a severe sensory and motor neuropathy. Two major splice variants, KCC3a and KCC3b, were shown to be expressed in adult mouse tissues. Although KCC3a is mainly expressed in the central nervous system (CNS), its specific cellular expression patterns have not been determined. Here, we used an approach combining in situ hybridization and immunohistochemical techniques to determine the cellular expression of KCC3 in the mouse CNS and showed that KCC3 is mainly expressed in neurons, including a subpopulation of interneurons. Finally, we showed that some non-neuronal cells, such as radial glial-like cells in the spinal cord, also express KCC3.
authors.affiliation: Centre of Excellence in Neuromics, CHUM Research Center and Department of Medicine, University of Montreal, Notre-Dame Hospital, 1560 Sherbrooke East, De-Seve Pavillion, room Y-3616-2, Montréal, QC, H2L 4M1, Canada.
authors.forename: Masoud
authors.initials: M
authors.lastname: Shekarabi
authors.forename: Adèle
authors.initials: A
authors.lastname: Salin-Cantegrel
authors.forename: Janet
authors.initials: J
authors.lastname: Laganière
authors.forename: Rébecca
authors.initials: R
authors.lastname: Gaudet
authors.forename: Patrick
authors.initials: P
authors.lastname: Dion
authors.forename: Guy A
authors.initials: GA
authors.lastname: Rouleau
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D005786:Gene Expression Regulation; D007395:Interneurons / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D009457:Neuroglia / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D020033:Protein Isoforms / Q000096:biosynthesis; D027981:Symporters / Q000096:biosynthesis*; D000096922:K Cl- Cotransporters
chemical_list: D020033:Protein Isoforms; D027981:Symporters; C492280:Slc12a6 protein, mouse; D000096922:K Cl- Cotransporters