title: [Expressions and significances of PRL-3 and RhoC in A549 cell].
abstract: OBJECTIVE
  the expression of phosphatase of regenerating liver-3 (PRL-3) is correlated with Ras homologue C (RhoC) in non-small cell lung cancer (NSCLC), suggesting that they have interactions. The aim of this study is to investigate the functions of PRL-3 and RhoC in the migration of A549 cell and the potential mechanism of PRL-3 and RhoC in carcinogenesis and cancer development..
  
  
  METHODS
  PRL-3Ab and RhoCAb were used to block the functions of PRL-3 and RhoC respectively. Wound healing assay was applied to detect the migration of A549 cell and the expression levels of PRL-3 and RhoC were detected by RT-PCR.
  
  
  RESULTS
  the migration of A549 cell decreased after blockage of PRL-3 and RhoC. The expression of RhoC decreased when PRL-3 was blocked without any changes on the expression of PRL-3.
  
  
  CONCLUSIONS
  PRL-3, RhoC could increase cell migration in A549 cells.
authors.affiliation: Department of Thoracic Surgery, Tangdu Hospital, the Fourth Military Medical University, Xi'an 710038, China.
authors.forename: Ping
authors.initials: P
authors.lastname: ZHANG
authors.forename: Zhipei
authors.initials: Z
authors.lastname: ZHANG
authors.forename: Xiangmin
authors.initials: X
authors.lastname: LI
authors.forename: Jie
authors.initials: J
authors.lastname: LEI
authors.forename: Kai
authors.initials: K
authors.lastname: SU
authors.forename: Xiaofei
authors.initials: X
authors.lastname: LI
authors.forename: Xiaoping
authors.initials: X
authors.lastname: WANG
mesh_terms: D000906:Antibodies / Q000494:pharmacology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects* / Q000235:genetics; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D017027:Protein Tyrosine Phosphatases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020741:rho GTP-Binding Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000075024:rhoC GTP-Binding Protein
chemical_list: D000906:Antibodies; D009363:Neoplasm Proteins; C484730:PTP4A3 protein, human; D017027:Protein Tyrosine Phosphatases; C066690:RHOC protein, human; D020741:rho GTP-Binding Proteins; D000075024:rhoC GTP-Binding Protein