title: Rapid responses of C14 clone of Eucalyptus globulus to root drought stress: Time-course of hormonal and physiological signaling.
abstract: The responses of juvenile plants of forest crops to drought stress are a key stage in the survival of forest populations. In this work, a suitable experimental system to study the early drought resistance mechanisms and signaling in a drought-tolerant clone (C14) of Eucalyptus globulus Labill is proposed. This system, using hydroponic culture and an osmotic agent, polyethylene glycol 8000, was demonstrated to induce severe stress in the root area, affecting the responses of the plantlets at the aerial level. These responses were very fast, beginning only 3h after the induction of stress, and the results highlight the roles of xylematic abscisic acid (ABA) and pH changes over other signals, such as cytokinins, as early chemical signals in rapid water stress. The relationship between these chemical factors, ABA and pH, and the physiological and water parameters observed were significant, supporting their proposed principal role. This work aids our understanding of underlying responses to hydrological limitations of forest crops, and provides valuable information for further physiological and molecular studies of water stress in this and other tree species.
authors.affiliation: Instituto Universitario de Biotecnología de Asturias, Universidad de Oviedo, 33071 Asturias, Spain. biovgg@gmail.com
authors.forename: Víctor
authors.initials: V
authors.lastname: Granda
authors.forename: Candela
authors.initials: C
authors.lastname: Cuesta
authors.forename: Rubén
authors.initials: R
authors.lastname: Alvarez
authors.forename: Ricardo
authors.initials: R
authors.lastname: Ordás
authors.forename: María Luz
authors.initials: ML
authors.lastname: Centeno
authors.forename: Ana
authors.initials: A
authors.lastname: Rodríguez
authors.forename: Juan Pedro
authors.initials: JP
authors.lastname: Majada
authors.forename: Belén
authors.initials: B
authors.lastname: Fernández
authors.forename: Isabel
authors.initials: I
authors.lastname: Feito
mesh_terms: D000040:Abscisic Acid / Q000302:isolation & purification / Q000378:metabolism; D003583:Cytokinins / Q000302:isolation & purification / Q000378:metabolism; D055864:Droughts; D005052:Eucalyptus / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D006863:Hydrogen-Ion Concentration; D010788:Photosynthesis; D018517:Plant Roots / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D003583:Cytokinins; D000040:Abscisic Acid