title: Multi-parameter determination of TNFα, PCT and CRP for point-of-care testing.
abstract: A proof of concept for the simultaneous multi-parameter determination of three inflammation and sepsis parameters-TNFα, PCT and CRP-using a compact optical immunosensor is demonstrated. Harmonized assay conditions revealed standard curves with test midpoints (IC(50)) of 380 µg L(-1) for TNFα, 2300 µg L(-1) for PCT, and 2645 µg L(-1) for CRP.
authors.affiliation: Helmholtz Zentrum München-German Research Center for Environmental Health, Institute of Ecological Chemistry, Neuherberg, Oberschleissheim, Germany. kramer@helmholtz-muenchen.de
authors.forename: Petra M
authors.initials: PM
authors.lastname: Krämer
authors.forename: Melanie
authors.initials: M
authors.lastname: Kess
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Kremmer
authors.forename: Sigurd
authors.initials: S
authors.lastname: Schulte-Hostede
mesh_terms: D015415:Biomarkers / Q000032:analysis / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D002097:C-Reactive Protein / Q000032:analysis* / Q000737:chemistry; D002116:Calcitonin / Q000032:analysis* / Q000737:chemistry; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007249:Inflammation / Q000378:metabolism; D007834:Lasers; D008970:Molecular Weight; D055620:Optical Phenomena; D019095:Point-of-Care Systems; D011498:Protein Precursors / Q000032:analysis* / Q000737:chemistry; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis* / Q000737:chemistry
chemical_list: D015415:Biomarkers; D011498:Protein Precursors; D014409:Tumor Necrosis Factor-alpha; D002116:Calcitonin; D002097:C-Reactive Protein