title: [The effect of danshen on the angiogenesis of the frozen-thawed human fetal ovarian tissue after transplantation].
abstract: OBJECTIVE
  (1) To investigate the mRNA expression of the key angiogenic growth factors in the grafts after transplantation. (2) To investigate the potential impact of danshen (Chinese traditional medicine) administration on grafts angiogenesis.
  
  
  METHODS
  The frozen-thawed ovarian tissue from aborted fetus were xenografted into the renal capsule of the nude mice, recovered 48 h, 7 d and 28 d after respectively. Either danshen or saline (as the control) was administered after transplantation.
  
  
  RESULTS
  The mRNA levels of VEGF showed a temporary raise in 48 h after transplantation, then decreased in one week, and no significant difference was fund between the control group and danshen group. Ang-2 was increased in 48 h after transplantation, when Danshen group was significantly higher than the control group (P < 0.05). The microvessel density significantly increased in all the tissues after transplantation. The control group peaked on day 7 after transplantation, while danshen group peaked in 48 h and kept correspondingly steady after that.
  
  
  CONCLUSIONS
  Early angiogenesis began within 48 h after transplantation of the thawed human fetal ovarian tissue, and its microvessel density peaked within the first week after transplantation. Our results also suggested that the use of danshen injection in conjunction with transplantation could facilitate revascularization of the grafts.
authors.affiliation: The First Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, China.
authors.forename: Dan-dan
authors.initials: DD
authors.lastname: Wu
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhou
authors.forename: Bing-sen
authors.initials: BS
authors.lastname: Xu
authors.forename: Yue-hong
authors.initials: YH
authors.lastname: Tong
authors.lastname: Lin-ju
mesh_terms: D042702:Angiopoietin-2 / Q000235:genetics / Q000378:metabolism; D000818:Animals; D015925:Cryopreservation; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D016332:Fetal Tissue Transplantation / Q000379:methods*; D005333:Fetus; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D006080:Ovarian Follicle / Q000166:cytology / Q000254:growth & development / Q000637:transplantation*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D027561:Salvia miltiorrhiza / Q000737:chemistry*; D014183:Transplantation, Heterologous / Q000379:methods; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism
chemical_list: D042702:Angiopoietin-2; D004365:Drugs, Chinese Herbal; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A