title: [The radioimmunoassay of growth hormone and prolactin and its application].
abstract: OBJECTIVE
  To set up a highly specific and sensitive double antibody radioimmunoassay (RIA) for determining the contents of growth hormone (GH) and prolactin (PRL) in pituitary and plasma of rats; To study the effects of acute hypoxia on GH and PRL of rats.
  
  
  METHODS
  We use chloramine-T technique iodinate antigen and choose the equilibration and saturated procedure of RIA to add the preparations.
  
  
  RESULTS
  Exposed to 7 km-hypoxia for 0.5 h, pituitary GH content increased (P < 0.05) and conversely, plasma GH levels were suppressed (P < 0.05); while the PRL levels in both pituitary and plasma were suppressed (P < 0.01); and there was no significantly difference between 5 km hypoxia groups and control.
  
  
  CONCLUSIONS
  We have successfully established the method of radiolabel and double antibody RIA with highly sensitivity; acute hypoxia (7 km) suppressed the secretion of GH and PRL in rats.
authors.affiliation: Department of Neurobiology and Physiology, College of Life Sciences, Zhejiang University, Hangzhou 310027, China.
authors.forename: Y S
authors.initials: YS
authors.lastname: Zhang
authors.forename: J Z
authors.initials: JZ
authors.lastname: Du
authors.forename: N Y
authors.initials: NY
authors.lastname: Xu
authors.forename: Y
authors.initials: Y
authors.lastname: Wu
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D013006:Growth Hormone / Q000097:blood / Q000378:metabolism*; D000860:Hypoxia / Q000378:metabolism*; D008297:Male; D010902:Pituitary Gland / Q000737:chemistry; D011388:Prolactin / Q000097:blood / Q000378:metabolism*; D011863:Radioimmunoassay / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D011388:Prolactin; D013006:Growth Hormone