title: [Immuno-blot detection of hemangiopoietin in the human fetal liver].
abstract: OBJECTIVE
  To detect the expression of a novel protein, hemangiopoietin (HAPO), in the human fetal liver at the protein level.
  
  
  METHODS
  Monoclonal antibodies (moAbs) against HAPO were produced by traditional hybridoma technique. Their affinities were calculated from the results of non-competitive ELISA and the antibodies were purified by protein G affinity chromatography. Expression of HAPO in the liver was detected by SDS-PAGE and Western blot.
  
  
  RESULTS
  Five strains of moAbs were screened out in total, among which three were IgG1 and the other two were IgM. Their light trains were all belonged to kappa. The relative affinities of the three IgG1 form moAbs were 3.06 x 10(9) mol/L, 6.07 x 10(8) mol/L and 1.71 x 10(10) mol/L respectively. After purification, the purity of the moAbs could reach more than 99%. HAPO expression was detected at the protein level in the human fetal liver, and the apparent molecular weight of the nature HAPO was very close to but a little higher than our recombinant one.
  
  
  CONCLUSIONS
  HAPO was expressed in the human fetal liver at the protein level.
authors.affiliation: Department of Pathophysiology, Xuzhou Medical College, Xuzhou 221002, China.
authors.forename: Ying-Song
authors.initials: YS
authors.lastname: Qin
authors.forename: Zhong-Chao
authors.initials: ZC
authors.lastname: Han
mesh_terms: D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D005333:Fetus; D006801:Humans; D015151:Immunoblotting; D008099:Liver / Q000196:embryology / Q000378:metabolism*; D011509:Proteoglycans / Q000378:metabolism*; D000099158:Proteoglycan 4
chemical_list: D000911:Antibodies, Monoclonal; D011509:Proteoglycans; C419197:PRG4 protein, human; D000099158:Proteoglycan 4