title: [The role of p38 on the differentiation of MSCs to myoblasts].
abstract: OBJECTIVE
  Inducing mesenchymal stem cells (MSCs) differentiate to myoblasts with 5-azacytidine(5-Aza-CR), investigating the expression of Myf5 and the role of the signal transduction case of p38 in all the course of differentiation.
  
  
  METHODS
  Separating and purifying bone marrow-derived MSCs, inducing MSCs differentiation to myoblasts with 10 micromol/L 5-Aza-CR, assaying the gene expression time of Myf5 with RT-PCR method, the antigen expression of myosin with immunohistochemistry method and observing the changes of the activity of phosphorylation p38 before and after inhibited by SB203580 with Western-blot method.
  
  
  RESULTS
  MSCs begin to express Myf5 delayed to the 9th day after inhibited by SB203580. Some of MSCs express myosin at the 7th day after induced; The phosphorylation p38 activity of MSCs enhanced after induced by 5-Aza-CR but obviously decreased after inhibited by SB203580.
  
  
  CONCLUSIONS
  MSCs can express myogenic regulator factors and orientation differentiate to myoblasts after induced by 5-Aza-CR, p38 really have a positive signal transduction affection in this course.
authors.affiliation: State Key Laboratory of Tranuma, Burns and Combined Injury, Institute of Combined Injury of PLA, College of Preventive Medicine, Third Military Medical University, Chongqing 400038.
authors.forename: Jin
authors.initials: J
authors.lastname: Wang
authors.forename: Cheng-Ji
authors.initials: CJ
authors.lastname: Luo
authors.forename: Xin-Ze
authors.initials: XZ
authors.lastname: Ran
authors.forename: Hui
authors.initials: H
authors.lastname: Xu
authors.forename: Yong-Ping
authors.initials: YP
authors.lastname: Su
authors.forename: Tian-Min
authors.initials: TM
authors.lastname: Cheng
mesh_terms: D000818:Animals; D001374:Azacitidine; D001854:Bone Marrow Cells / Q000166:cytology; D002454:Cell Differentiation; D002478:Cells, Cultured; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D008807:Mice, Inbred BALB C; D032446:Myoblasts / Q000166:cytology*; D051787:Myogenic Regulatory Factor 5 / Q000378:metabolism; D015398:Signal Transduction; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism*
chemical_list: C497418:Myf5 protein, mouse; D051787:Myogenic Regulatory Factor 5; D048051:p38 Mitogen-Activated Protein Kinases; D001374:Azacitidine