title: Evaluation of the bioactivity of novel spiroisoxazoline typecompounds against normal and cancer cell lines.
abstract: The aim of this study was to investigate the in vitro cellular activity of novel spiroisoxazoline type compounds against normal and cancer cell lines from lung tissue (Hs888Lu), neuron-phenotypic cells (SH-SY5Y), neuroblastoma (SH-SY5Y), human histiocytic lymphoma (U937), lung cancer (A549), and leukaemia (HL-60). Our bioassay program revealed that the spiroisoxazoline type compounds show cytotoxicity only in lymphoma cell lines, which is in contrast with the pyrrolidine precursor of these spiroisoxazoline compounds, where significant cytotoxicity is seen in all normal and cancer cell lines. These data suggest a tumour-specific mechanism of action. In addition these data also show that spiroisoxazoline compounds are non-toxic in the human neuronphenotypic neuroblastoma SH-SY5Y cell line, and furthermore that they might protect cells from neurodegenerative disease.
authors.affiliation: Tissue Culture Research Laboratory, Centre of Synthesis and Chemical Biology, Institute of Science, University Technology MARA, 40450 Shah Alam, Malaysia. nigar@salam.uitm.edu.my
authors.forename: Nigar
authors.initials: N
authors.lastname: Najim
authors.forename: Yaser
authors.initials: Y
authors.lastname: Bathich
authors.forename: Mazatulikhma Mat
authors.initials: MM
authors.lastname: Zain
authors.forename: Ahmad Sazali
authors.initials: AS
authors.lastname: Hamzah
authors.forename: Zurina
authors.initials: Z
authors.lastname: Shaameri
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology*; D001681:Biological Assay / Q000379:methods; D003603:Cytotoxins / Q000737:chemistry / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor; D018922:HL-60 Cells; D006801:Humans; D009369:Neoplasms / Q000188:drug therapy*; D019636:Neurodegenerative Diseases / Q000188:drug therapy; D011759:Pyrrolidines / Q000737:chemistry* / Q000494:pharmacology*; D020298:U937 Cells
chemical_list: D000970:Antineoplastic Agents; D003603:Cytotoxins; D011759:Pyrrolidines