title: Toxic effects of different charged metal ions on the target--bovine serum albumin.
abstract: In this work, the toxic influence of metallic ions (Na+, Cu2+, Al3+) on the serum albumin were studied by fluorescence, resonance light scattering (RLS), synchronous fluorescence, UV-vis absorption and circular dichroism (CD) spectroscopy. The experimental results indicated that ion electric charge is not the main factor affecting the structure of bovine serum albumin (BSA). Na+ made the structure of BSA tighter and hydrophobicity enhanced, which improved fluorescence intensity, while Cu2+ could react with some functional groups of BSA, making the structure of BSA looser, so that the internal hydrophobic groups such as tryptophan (Trp) and other aromatic residues were gradually exposed. When we observed them with fluorescence spectra, we found fluorescence quenching with increasing Cu2+ dose. Al3+ is shown as little significant influence on the BSA, but BSA was found to aggregate with the dose of Al3+ by means of RLS because of the hydrolysis and ion strength effect of Al3+. The results also proved normal saline could keep lives healthy and good-working as a biological humour, however, heavy metals made harmful effects to the body when they exceeded the minimal effect level (MEL), such as Cu2+ chosen in our work.
authors.affiliation: Shandong Key Laboratory of Water Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Shandong University, Jinan 250100, PR China.
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.forename: Rutao
authors.initials: R
authors.lastname: Liu
authors.forename: Zhenxing
authors.initials: Z
authors.lastname: Chi
authors.forename: Canzhu
authors.initials: C
authors.lastname: Gao
mesh_terms: D000535:Aluminum / Q000633:toxicity; D000818:Animals; D002417:Cattle; D003300:Copper / Q000633:toxicity; D004583:Electrons; D006860:Hydrogen Bonding / Q000187:drug effects; D007477:Ions; D008670:Metals / Q000633:toxicity*; D008968:Molecular Conformation; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000187:drug effects*; D012964:Sodium / Q000633:toxicity; D017550:Spectroscopy, Fourier Transform Infrared; D013329:Structure-Activity Relationship; D013816:Thermodynamics; D014732:Vibration
chemical_list: D007477:Ions; D008670:Metals; D012710:Serum Albumin, Bovine; D003300:Copper; D012964:Sodium; D000535:Aluminum