title: [Changes of scavenger receptor class B type I and peroxisome proliferator-activated receptor gamma expression in atherosclerotic mini swine].
abstract: OBJECTIVE
  To study the expressions of scavenger receptor class B type I(SR-BI) and peroxisome proliferator-activated receptor gamma (PPARgamma) in atherosclerotic mini swine and provide a new mechanism for investigating the pathogenesis of atherosclerosis.
  
  
  METHODS
  Chinese mini swine were fed by a normal control diet or a high fat/high cholesterol diet for 12 months after common carotid artery injury induced by balloon denudation. Plasma total cholesterol(TC), high-density lipoprotein cholesterol (HDL-C) and triglycerides (TG) were determined by commercially enzymatic methods every two months. The sections, which were taken from liver and abdominal aorta, were stained with hematoxylin eosin. The expressions of SR-BI and PPARgamma mRNA and protein in liver and aorta tissue were detected by reverse transcriptase-polymerase chain reaction (RT-PCR), Western blot and immunohistochemistry respectively.
  
  
  RESULTS
  At the end of 12 months, plasma TC, HDL-C and TG in HFHC mini swine were increased. There were fatty liver and atherosclerotic plaque in mini swine live and aorta respectively. The expression of SR-BI was upregulated in HFHC mini swine liver and aorta tissue.
  
  
  CONCLUSIONS
  HFHC may induce atherosclerosis and the expression of SR-BI and PPARgamma. Upregulating SR-BI expression may inhibit atherosclerosis. Increasing SR-BI expression in liver and aorta may accelerate SR-BI-mediated reverse cholesterol transport and develop a new anti-atherogenic strategy.
authors.affiliation: Institute of Cardiovascular Research, Nanhua University, Hengyang 421001, China.
authors.forename: Guang-Hui
authors.initials: GH
authors.lastname: Yi
authors.forename: Zhong-Cheng
authors.initials: ZC
authors.lastname: Mo
authors.forename: Ying
authors.initials: Y
authors.lastname: Zeng
authors.forename: Xiao-Bo
authors.initials: XB
authors.lastname: Yin
authors.forename: Lu-Shan
authors.initials: LS
authors.lastname: Liu
authors.forename: Zuo
authors.initials: Z
authors.lastname: Wang
authors.forename: Jing-Tao
authors.initials: JT
authors.lastname: Feng
authors.forename: De-Xing
authors.initials: DX
authors.lastname: Zeng
authors.forename: Lin
authors.initials: L
authors.lastname: Sun
mesh_terms: D000818:Animals; D001161:Arteriosclerosis / Q000473:pathology; D050197:Atherosclerosis / Q000378:metabolism*; D047495:PPAR gamma / Q000378:metabolism*; D051116:Receptors, Scavenger / Q000378:metabolism*; D013552:Swine
chemical_list: D047495:PPAR gamma; D051116:Receptors, Scavenger