title: [The effect of NO, ET-1 on brain injury after hind limbs ischemia/reperfusion in rats].
abstract: OBJECTIVE
  To study the roles of nitric oxide (NO) and ET-1 in brain injury after hind limbs ischemia/reperfusion in rats and to investigate the effect of NO/ ET-1 balance on brain injury.
  
  
  METHODS
  On a model of the hind limbs ischemia/reperfusion (LI/R) of rats, we used L-Arg(L-arginine, L-Arg), one of the substrates in the process of nitric oxide, aminoguanidine (AG) which inhibits nitric oxide synthase(NOS) and ETA receptor antagonist BQ123, to observe the changes of NO, ET-1, MDA, XOD, SOD, LDH in plasma and tNOS, iNOS, cNOS, NO, ET-1, MDA, XOD, MPO, SOD in brain tissue.
  
  
  RESULTS
  Compared with the control group, the content of MDA, XOD, LDH in plasma and MDA, XOD, MPO in brain tissue increased. The activity of SOD decreased (P < 0.01). The content of tNOS, iNOS in brain tissue increased, cNOS decreased (P < 0.01). The content of NO, ET-1 in I/R group in plasma and brain tissue increased, the ratio of NO/ET-1 decreased. The brain injury was deteriorated. After using L-Arg and BQ123, the ratio of NO/ET-1 in plasma and brain tissue increased, the brain injury lightened. Whereas after using AG, the ratio of NO/ET-1 decreased, brain injury became more serious.
  
  
  CONCLUSIONS
  The NO/ET-1 ratio decreased after LI/R, brain injury became more serious.
authors.affiliation: Department of Pathophysiology North China Coal Medical College, Tangshan 063000, China.
authors.forename: Yong-qi
authors.initials: YQ
authors.lastname: Hu
authors.forename: Lian-yuan
authors.initials: LY
authors.lastname: Zhang
authors.forename: Hong-jie
authors.initials: HJ
authors.lastname: Li
authors.forename: Jun
authors.initials: J
authors.lastname: Peng
authors.forename: Shu-yun
authors.initials: SY
authors.lastname: Dong
authors.forename: Xiu-li
authors.initials: XL
authors.lastname: Men
authors.forename: Quan-hui
authors.initials: QH
authors.lastname: Yang
authors.forename: Hong-xia
authors.initials: HX
authors.lastname: Zhou
mesh_terms: D000818:Animals; D001930:Brain Injuries / Q000473:pathology*; D019332:Endothelin-1 / Q000378:metabolism*; D005121:Extremities / Q000098:blood supply; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000378:metabolism* / Q000473:pathology
chemical_list: D019332:Endothelin-1; D009569:Nitric Oxide