title: Functional composite nanofibers of poly(lactide-co-caprolactone) containing gelatin-apatite bone mimetic precipitate for bone regeneration.
abstract: Functional nanofibrous materials composed of gelatin-apatite-poly(lactide-co-caprolactone) (PLCL) were produced using an electrospinning process. A gelatin-apatite precipitate, which mimicked bone extracellular matrix, was homogenized in an organic solvent using various concentrations of PLCL. A fibrous structure with approximate diameters of a few hundred nanometers was successfully generated. Apatite nanocrystallines were found to be effectively distributed within the polymeric matrix of the gelatin-PLCL. The addition of a small amount of gelatin-apatite into PLCL significantly improved the tensile strength of the nanofiber by a factor of 1.8. Moreover, tissue cell growth on the composite nanofiber was enhanced. Osteogenic differentiation of the cells was significantly stimulated by the composite nanofiber compared with the pure PLCL nanofiber. When implanted in a rat calvarium for 6weeks the composite nanofiber supported defect closure and new bone formation better than the pure PLCL nanofiber, as deduced from micro-computed tomography and histological analyses. Based on these results, the gelatin-apatite-PLCL composite nanofiber developed in this study is considered to be potentially useful as a bone tissue regeneration matrix.
authors.affiliation: Department of Nanobiomedical Science and WCU Research Center, Dankook University, South Korea.
authors.forename: Seung-Hwan
authors.initials: SH
authors.lastname: Jegal
authors.forename: Jeong-Hui
authors.initials: JH
authors.lastname: Park
authors.forename: Joong-Hyun
authors.initials: JH
authors.lastname: Kim
authors.forename: Tae-Hyun
authors.initials: TH
authors.lastname: Kim
authors.forename: Ueon Sang
authors.initials: US
authors.lastname: Shin
authors.forename: Tae-Il
authors.initials: TI
authors.lastname: Kim
authors.forename: Hae-Won
authors.initials: HW
authors.lastname: Kim
mesh_terms: D000818:Animals; D001031:Apatites / Q000494:pharmacology*; D001861:Bone Regeneration / Q000187:drug effects*; D001842:Bone and Bones / Q000000981:diagnostic imaging / Q000187:drug effects* / Q000473:pathology / Q000601:surgery; D011232:Chemical Precipitation / Q000187:drug effects*; D005780:Gelatin / Q000494:pharmacology*; D008422:Materials Testing; D051379:Mice; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects; D011091:Polyesters / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013194:Staining and Labeling; D013718:Tensile Strength / Q000187:drug effects; D055114:X-Ray Microtomography
chemical_list: D001031:Apatites; D011091:Polyesters; C553659:polycaprolactone-co-lactide; D005780:Gelatin