title: [Study on protective effect of vitamin E for ovarian grandlose cells and its mechanism in aged rats].
abstract: OBJECTIVE
  To observe the effect of vitamin E (VE) on ovarian apoptosis-related protein Bcl-2 and Bax and its impact on antioxidant capacity in aged female rats and to study the senility-delaying effect and mechanism of VE on ovary.
  
  
  METHODS
  Natural aging female rats were given different doses of exogenous VE. Then apoptosis regulatory protein Bcl-2, Bax expression in ovarian grandlose cells were detected by using immunohistochemical methods and Western blot. The contents of serum total superoxide dismutase (SOD) activity and malondialdehyde (MDA) were detected by using biochemical methods.
  
  
  RESULTS
  Contrasted with adult control group, the level of Bcl-2 expression in Senile control group was lower and the level of Bax expression was higher (P < 0.01), Serum SOD activity decreased and the level of MDA significantly increased (P < 0.01). Contrasted with senile control group, the level of Bcl-2 expression increased in VE group, the level of Bax expression decreased (P < 0.05), the level of MDA expression significantly decreased (P < 0.01).
  
  
  CONCLUSIONS
  VE can regulate apoptosis-related protein Bcl-2, Bax expression and confront free radical damage which contribute to a protective effect for ovarian grandiose cells.
authors.affiliation: Department of Physiology, Qiqihar Medical College, Qiqihar 161006, China. luchangzhu_1998@tom.com
authors.forename: Chang-Zhu
authors.initials: CZ
authors.lastname: Lu
authors.forename: Yan-Hui
authors.initials: YH
authors.lastname: Shi
authors.forename: Run-Sheng
authors.initials: RS
authors.lastname: Jiao
authors.forename: Ya-Kun
authors.initials: YK
authors.lastname: Kong
authors.forename: Lan-Chun
authors.initials: LC
authors.lastname: Yao
mesh_terms: D000375:Aging; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D005260:Female; D006107:Granulosa Cells / Q000166:cytology*; D010053:Ovary / Q000166:cytology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D014810:Vitamin E / Q000494:pharmacology*; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: D000975:Antioxidants; C494828:Bax protein, rat; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D014810:Vitamin E