title: Combined hyperbaric oxygen and hypothermia treatment on oxidative stress parameters after spinal cord injury: an experimental study.
abstract: OBJECTIVE
  We undertook this study to investigate the possible beneficial effects of combined hypothermia and hyperbaric oxygen (HBO) treatment in comparison with methylprednisolone in experimental spinal cord injury (SCI).
  
  
  METHODS
  Forty eight male Wistar albino rats (200-250 g) were randomized into six groups; A (normothermic control group; only laminectomy), B (normothermic trauma group; laminectomy + spinal trauma), C (normothermic methylprednisolone group; laminectomy + spinal trauma + methylprednisolone treated), D (hypothermia group; laminectomy + spinal trauma + hypothermia treated); E (HBO group; laminectomy + spinal trauma + HBO therapy), F (hypothermia and HBO group; laminectomy + spinal trauma + hypothermia and HBO treated) each containing eight rats. Neurological assessments were performed 24 h after trauma and spinal cord tissue samples had been harvested for both biochemical and histopathological evaluation.
  
  
  RESULTS
  After SCI, tissue malondialdehyde (MDA) level of the control group was measured increased, and superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) enzyme activities were measured decreased. In group F, it was also shown that MDA level elevation had been prevented, and group F has increased the antioxidant enzyme activities than the other experimental groups C, D, E (p <0.05).
  
  
  CONCLUSIONS
  We concluded that the use of combined hypothermia and HBO treatment might have potential benefits in spinal cord tissue on secondary damage.
authors.affiliation: Department of Neurosurgery, Haydarpasa Training Hospital, Gulhane Military Medical Academy, Istanbul, Turkey.
authors.forename: Kivanc
authors.initials: K
authors.lastname: Topuz
authors.forename: Ahmet
authors.initials: A
authors.lastname: Colak
authors.forename: Berker
authors.initials: B
authors.lastname: Cemil
authors.forename: Murat
authors.initials: M
authors.lastname: Kutlay
authors.forename: Mehmet Nusret
authors.initials: MN
authors.lastname: Demircan
authors.forename: Hakan
authors.initials: H
authors.lastname: Simsek
authors.forename: Osman
authors.initials: O
authors.lastname: Ipcioglu
authors.forename: Zafer
authors.initials: Z
authors.lastname: Kucukodaci
authors.forename: Gunalp
authors.initials: G
authors.lastname: Uzun
mesh_terms: D000818:Animals; D006931:Hyperbaric Oxygenation; D007036:Hypothermia, Induced; D007796:Laminectomy; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013116:Spinal Cord / Q000378:metabolism / Q000473:pathology; D013119:Spinal Cord Injuries / Q000378:metabolism / Q000473:pathology / Q000628:therapy*
chemical_list: D008315:Malondialdehyde