title: [Effect of MK801 on apoptosis in the development of brain injury after hind limbs ischemia/reperfusion in rats].
abstract: OBJECTIVE
  To evaluate development of brain injury after hind limbs ischemia/reperfusion (LI/R) in rats, and the effect of MK801 on the brain injury following LI/R.
  
  
  METHODS
  The limbs ischemia/reperfusion model was established in rats. The MDA contents were evaluated in each group, apoptotic cells were detected with TUNEL, the expression of apoptosis-associated protein, such as bcl-2, cytoC and caspase-3 were determined with immunohistochemistry and Western-blot.
  
  
  RESULTS
  The contents of MDA in brain tissue increased significantly following LI/R. The expression of bcl-2, cytoC, Caspase-3 was increased than those in the control group (P < 0.01) following LI/R significantly. The expression of Caspase-3 was increased 24 h after the onset of reperfusion. The expression of Caspase-3, bcl-2 gene was quite obvious in the midbrain red nucleus region. MK801 inhibited the expression of bcl-2, cytoC, Caspase-3 obviously.
  
  
  CONCLUSIONS
  The excessive apoptosis and apoptosis-associated factors could play an important role in the brain injury following LI/R in rat, MK801 might decrease the production of free radical and the excite toxicity of glutamate, inhibit the expression of apoptosis associated protein and reduce the occurrence of apoptosis.
authors.affiliation: Department of Basic Medical Sciences, North China Coal Medical College, Tangshan 063000, China. zhx2729315@163.com
authors.forename: Hong-Xia
authors.initials: HX
authors.lastname: Zhou
authors.forename: Lian-Yuan
authors.initials: LY
authors.lastname: Zhang
authors.forename: Tian
authors.initials: T
authors.lastname: Zhang
authors.forename: Shu-Yun
authors.initials: SY
authors.lastname: Dong
authors.forename: Yu-Xin
authors.initials: YX
authors.lastname: Zhang
mesh_terms: D000818:Animals; D017209:Apoptosis; D001930:Brain Injuries / Q000378:metabolism / Q000473:pathology*; D053148:Caspase 3 / Q000378:metabolism; D045304:Cytochromes c / Q000378:metabolism; D016291:Dizocilpine Maleate / Q000494:pharmacology*; D005121:Extremities / Q000098:blood supply; D007511:Ischemia; D008297:Male; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism / Q000473:pathology*
chemical_list: D019253:Proto-Oncogene Proteins c-bcl-2; D016291:Dizocilpine Maleate; D045304:Cytochromes c; C505410:Casp3 protein, rat; D053148:Caspase 3