title: In vivo evaluation of anthelmintic potential of medicinal plant extracts against Dactylogyrus intermedius (Monogenea) in goldfish (Carassius auratus).
abstract: In the present study, an attempt has been made to petroleum ether, chloroform, ethyl acetate, methanol, and water extracts of Prunus amygdalus Batsch seeds (Semen amygdali), Cimicifuga foetida L. rhizomes (Rhizoma Cimicifugae), Peucedanum decursivum (Miq.) Maxim roots (Radix Peucedani), Momordica cochinchinensis Spreng. seeds (Semen Momordicae), and Bupleurum chinense DC. roots (Radix Bupleuri chinensis) for their in vivo anthelmintic activity against monogenean Dactylogyrus intermedius in goldfish (Carassius auratus). The results showed that the efficacies of methanol, chloroform, and ethyl acetate extracts from R. Bupleuri chinensis were found to be, in this order, more effective than others with the 48 h-EC(50) and EC(90) values of 3.5 and 6.9, 6.0 and 8.4, 7.4 and 11.2 mg/L, respectively, followed by ethyl acetate extract of R. cimicifugae and chloroform extract of R. peucedani with EC(50) 189.2 and 240.4 mg/L. The promising methanol, chloroform, and ethyl acetate extracts from R. Bupleuri chinensis were subjected to acute toxicity tests for the evaluation of their safety to the host. After 48-h exposure, the mortalities of goldfish were recorded, and the established LC(50) values were 10.1-, 4.2-, and 8.4-fold higher than the corresponding EC(50). These results indicated that the three extracts from R. Bupleuri chinensis exhibit potential to be used as preferred natural antiparasitics for the control of the D. intermedius, especially for the methanolic one.
authors.affiliation: Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi, 712100, China.
authors.forename: Zong-Fan
authors.initials: ZF
authors.lastname: Wu
authors.forename: Bin
authors.initials: B
authors.lastname: Zhu
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.forename: Cheng
authors.initials: C
authors.lastname: Lu
authors.forename: Gao-Xue
authors.initials: GX
authors.lastname: Wang
mesh_terms: D000818:Animals; D000980:Antiplatyhelmintic Agents / Q000494:pharmacology* / Q000627:therapeutic use; D002590:Cestode Infections / Q000188:drug therapy*; D005393:Fish Diseases / Q000188:drug therapy* / Q000469:parasitology; D006054:Goldfish / Q000469:parasitology*; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D010946:Plants, Medicinal; D010986:Platyhelminths / Q000187:drug effects*
chemical_list: D000980:Antiplatyhelmintic Agents; D010936:Plant Extracts