title: Ochrobactrum daejeonense sp. nov., a nitrate-reducing bacterium isolated from sludge of a leachate treatment plant.
abstract: A Gram-reaction-negative, non-spore-forming, rod-shaped, aerobic bacterial strain, designated MJ11(T), was isolated from sludge of a leachate treatment plant in Daejeon, South Korea, and was characterized taxonomically by using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that strain MJ11(T) belonged to the family Brucellaceae, class Alphaproteobacteria, and was most closely related to Ochrobactrum ciceri Ca-34(T) (97.9 % sequence similarity) and Ochrobactrum pituitosum CCUG 50899(T) (96.4 %). Comparative sequence analyses of the additional phylogenetic marker genes dnaK, groEL and gyrB confirmed the affiliation of strain MJ11(T) to the genus Ochrobactrum. The G+C content of the genomic DNA of strain MJ11(T) was 59.3 mol%. The detection of a quinone system with ubiquinone Q-10 as the predominant respiratory lipoquinone, a fatty acid profile with C(18 : 1)ω7c (62.6 %) and C(19 : 0) cyclo ω8c (14.2 %) as the major components, a polar lipid profile with phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylmonomethylethanolamine, diphosphatidylglycerol and unknown aminolipids AL1 and AL2 as major polar lipids and spermidine and putrescine as the predominant polyamines also supported the affiliation of strain MJ11(T) to the genus Ochrobactrum. The DNA-DNA relatedness between strain MJ11(T) and Ochrobactrum ciceri DSM 22292(T) was 29 ± 7 %, clearly showing that the isolate constitutes a new genospecies. Strain MJ11(T) could be clearly differentiated from its closest neighbours on the basis of its phenotypic, genotypic and chemotaxonomic features. Therefore, strain MJ11(T) represents a novel species of the genus Ochrobactrum, for which the name Ochrobactrum daejeonense sp. nov. is proposed. The type strain is MJ11(T) ( = KCTC 22458(T) = JCM 16234(T)).
authors.affiliation: School of Civil and Environmental Engineering, Yonsei University, Seoul 120-749, Republic of Korea.|Research and Development Division, H-Plus Eco Ltd, BVC 301, KRIBB, Eoeun-dong, Yuseong-gu, Daejeon 305-333, Republic of Korea.
authors.forename: Sung-Geun
authors.initials: SG
authors.lastname: Woo
authors.affiliation: Research and Development Division, H-Plus Eco Ltd, BVC 301, KRIBB, Eoeun-dong, Yuseong-gu, Daejeon 305-333, Republic of Korea.
authors.forename: Leonid N
authors.initials: LN
authors.lastname: Ten
authors.affiliation: School of Civil and Environmental Engineering, Yonsei University, Seoul 120-749, Republic of Korea.
authors.forename: Joonhong
authors.initials: J
authors.lastname: Park
authors.affiliation: Research and Development Division, H-Plus Eco Ltd, BVC 301, KRIBB, Eoeun-dong, Yuseong-gu, Daejeon 305-333, Republic of Korea.
authors.forename: Myungjin
authors.initials: M
authors.lastname: Lee
mesh_terms: D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005227:Fatty Acids / Q000378:metabolism; D008969:Molecular Sequence Data; D009566:Nitrates / Q000378:metabolism*; D042041:Ochrobactrum / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D010084:Oxidation-Reduction; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D012722:Sewage / Q000382:microbiology*; D014865:Waste Disposal, Fluid
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D009566:Nitrates; D012336:RNA, Ribosomal, 16S; D012722:Sewage