title: [Effects of lactate on cell injury in primary cultures of rat cortical neurons during hypoxia/reoxygenation].
abstract: OBJECTIVE
  To explore the effects of different concentrations of lactate on neuronal injury during hypoxia/reoxygenation (H/R) and its mechanism.
  
  
  METHODS
  Different concentrations of lactate (0, 0.5, 1.0, 2.0, 5.0 mmol/L) were added into medium after different duration of hypoxia, then reoxygenation for 24 h, cell survival rate and LDH release were assayed to determine neuronal damage, moreover, equal concentration of hydrochloric acid were used to mimic the changes of pH brought by lactate for investigating the mechanism of the effects of lactate on neuronal H/R injury.
  
  
  RESULTS
  Under normoxia and H/R 5.0 mmol/L lactate and hydrochloric acid induced or exacerbated neuronal injury. After 12 h and 24 h hypoxia exposure 1.0 mmol/L lactate was shown to be protective, 1.0 mmol/L hydrochloric acid had no effect on neuronal H/R damage.
  
  
  CONCLUSIONS
  Lactate of lower concentration was demonstrated to be neuroprotective during H/R, this protective effect was shown to be due to lactate anions. In contrast, higher concentration of lactate could induce or aggravate neuronal damage under normoxia and H/R, perhaps via the mechanism which involved the changes of pH.
authors.affiliation: Institute of Health and Environmental Medicine, Academy of Military Medical Sciences, Tianjin 300050, China.
authors.forename: Jing-wen
authors.initials: JW
authors.lastname: Wang
authors.forename: Jia-ying
authors.initials: JY
authors.lastname: Liu
authors.forename: Pei-hua
authors.initials: PH
authors.lastname: Yan
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Hai
authors.initials: H
authors.lastname: Wang
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D015687:Cell Hypoxia; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology*; D019344:Lactic Acid / Q000494:pharmacology*; D009474:Neurons / Q000166:cytology*; D018696:Neuroprotective Agents / Q000494:pharmacology*; D061251:Primary Cell Culture; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000517:prevention & control*
chemical_list: D018696:Neuroprotective Agents; D019344:Lactic Acid