title: Association analysis of polymorphisms of porcine LMP2 and LMP7 genes with haematological traits.
abstract: Low molecular weight polypeptides 2 (LMP2) and low molecular weight polypeptides 7 (LMP7) are located within the major histocompatibility complex and have been associated with autoimmune disease. In this study, polymorphisms of porcine LMP2 and LMP7 genes were analyzed by PCR-SSCP and DNA sequencing methods. Four SNPs (DQ659151:g.2115T>C; DQ659151:g.4343A>G; DQ872631:g.1232C>G; DQ872631:g.2847C>T) were identified. Four SNPs of genes were analyzed for association with 22 haematological traits in Large White (n = 195), Landrace (n = 84) and Songliao Black (n = 86) pig population. Of all the 22 traits, seven were significant associated with the SNPs of LMP2/LMP7 gene (P < 0.05). They included white blood cell count (WBC) (P = 0.028), neutrophilic granulocyte count (GRAN) (P = 0.037), monocytes percentage (MO%) (P = 0.015), red blood cell (RBC) (P = 0.004), red blood cell volume distribution width (RDW) (P = 0.004), mean platelet volume (MPV) (P = 0.016) and CD4(+)CD8(+)% (P = 0.045). These results suggest LMP2/LMP7 gene should be regarded as molecular marker to estimate animal's immune status for their effects on hematological traits.
authors.affiliation: Key Laboratory Animal Genetics and Breeding of the Ministry of Agricultural, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Liu
authors.forename: Y R
authors.initials: YR
authors.lastname: Luo
authors.forename: X
authors.initials: X
authors.lastname: Lu
authors.forename: X T
authors.initials: XT
authors.lastname: Qiu
authors.forename: J P
authors.initials: JP
authors.lastname: Zhou
authors.forename: Y F
authors.initials: YF
authors.lastname: Gong
authors.forename: X D
authors.initials: XD
authors.lastname: Ding
authors.forename: Q
authors.initials: Q
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D033481:Chromosomes, Mammalian / Q000235:genetics; D003546:Cysteine Endopeptidases / Q000235:genetics; D005787:Gene Frequency / Q000235:genetics; D056726:Genetic Association Studies; D005838:Genotype; D016018:Least-Squares Analysis; D018895:Microsatellite Repeats / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D018807:Polymorphism, Single-Stranded Conformational / Q000235:genetics; D046988:Proteasome Endopeptidase Complex / Q000235:genetics*; D019655:Quantitative Trait, Heritable; D034421:Sus scrofa / Q000097:blood* / Q000235:genetics*
chemical_list: C071313:LMP-2 protein; D003546:Cysteine Endopeptidases; C077436:LMP7 protein; D046988:Proteasome Endopeptidase Complex