title: Beta-1,3-glucanase from Delftia tsuruhatensis strain MV01 and its potential application in vinification.
abstract: During vinification microbial activities can spoil wine quality. As the wine-related lactic acid bacterium Pediococcus parvulus is able to produce slimes consisting of a β-1,3-glucan, must and wine filtration can be difficult or impossible. In addition, the metabolic activities of several wild-type yeasts can also negatively affect wine quality. Therefore, there is a need for measures to degrade the exopolysaccharide from Pediococcus parvulus and to inhibit the growth of certain yeasts. We examined an extracellular β-1,3-glucanase from Delftia tsuruhatensis strain MV01 with regard to its ability to hydrolyze both polymers, the β-1,3-glucan from Pediococcus and that from yeast cell walls. The 29-kDa glycolytic enzyme was purified to homogeneity. It exhibited an optimal activity at 50°C and pH 4.0. The sequencing of the N terminus revealed significant similarities to β-1,3-glucanases from different bacteria. In addition, the investigations indicated that this hydrolytic enzyme is still active under wine-relevant parameters such as elevated ethanol, sulfite, and phenol concentrations as well as at low pH values. Therefore, the characterized enzyme seems to be a useful tool to prevent slime production and undesirable yeast growth during vinification.
authors.affiliation: Institute of Microbiology and Wine Research, Johann-Joachim-Becherweg 15, 55128 Mainz, Germany.
authors.forename: V
authors.initials: V
authors.lastname: Blättel
authors.forename: M
authors.initials: M
authors.lastname: Larisika
authors.forename: P
authors.initials: P
authors.lastname: Pfeiffer
authors.forename: C
authors.initials: C
authors.lastname: Nowak
authors.forename: A
authors.initials: A
authors.lastname: Eich
authors.forename: J
authors.initials: J
authors.lastname: Eckelt
authors.forename: H
authors.initials: H
authors.lastname: König
mesh_terms: D002473:Cell Wall / Q000378:metabolism; D042742:Delftia / Q000145:classification / Q000201:enzymology*; D019649:Food Industry / Q000592:standards; D005519:Food Preservation / Q000379:methods*; D006026:Glycoside Hydrolases / Q000378:metabolism*; D010376:Pediococcus / Q000254:growth & development / Q000378:metabolism; D014920:Wine / Q000382:microbiology* / Q000592:standards; D015003:Yeasts / Q000254:growth & development / Q000378:metabolism; D047071:beta-Glucans / Q000378:metabolism*
chemical_list: D047071:beta-Glucans; C033363:beta-1,3-glucan; D006026:Glycoside Hydrolases