title: [The changes of cAMP and adenylyl cyclase mRNA level in hippocampus of mice with ischemia/reperfusion].
abstract: OBJECTIVE
  To observe the cAMP and adenylyl cyclase (AC) mRNA level in hippocampus of mice with vascular dementia (ischemia/reperfusion), and explore the molecular pathogenesis of ischemia/reperfusion.
  
  
  METHODS
  The mice were subjected for ischemia/reperfusion three times on bilateral common carotid arteries by knots to establish models of ischemia/reperfusion and the changes of learning and memory were tested on 29 d/30 d after operation. Sham-operation mice were introduced as control group. The cAMP level was evaluated by the radioimmunoassay (RIA), AC mRNA positive neurons of hippocampus CA1 area were dyed through in-situ hybridization (ISH).
  
  
  RESULTS
  Compared with sham-operation group, the learning and memory of model group was worse (P < 0.05), the cAMP level in hippocampus was lower (P < 0.05) and the surface density (Sv) of AC mRNA positive neurons reduced (P < 0.05).
  
  
  CONCLUSIONS
  The lower cAMP and AC mRNA level in hippocampus might participate in the molecular pathogenesis of ischemia/reperfusion.
authors.affiliation: Department of Neurology, Shandong Jining First People's Hospital, Jining 272111, China.
authors.forename: Wei-Bin
authors.initials: WB
authors.lastname: Wang
authors.forename: Chun-Feng
authors.initials: CF
authors.lastname: Song
authors.forename: Pei-Yuan
authors.initials: PY
authors.lastname: Lu
authors.forename: Yu
authors.initials: Y
authors.lastname: Yin
authors.forename: Cui-Ping
authors.initials: CP
authors.lastname: Liang
mesh_terms: D000262:Adenylyl Cyclases / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D000242:Cyclic AMP / Q000235:genetics / Q000378:metabolism*; D006624:Hippocampus / Q000378:metabolism*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D012333:RNA, Messenger / Q000235:genetics; D015427:Reperfusion Injury / Q000235:genetics / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D000242:Cyclic AMP; D000262:Adenylyl Cyclases