title: [Effects of zinc on the expression of metallothionein isoforms in stressed hippocampal neurons in vitro].
abstract: OBJECTIVE
  To evaluate the effects of different doses of zinc on the expression of metallothionein isoforms in stressed hippocampal neurons in vitro.
  
  
  METHODS
  The cell stress model was developed by corticosterone. The cultured hippocampal neurons were assigned to seven groups as follows: control group, zinc deficiency group, and their corresponding stressed groups, as well as three different levels of zinc complementarity groups.
  
  
  RESULTS
  In zinc deficiency group, the expressions of metallothionein and MT-1 mRNA, MT-3 mRNA were downregulated. On the other hand, inductions of metallothionein and it's mRNAs in stressed zinc complementarity group were increased. In addition, the levels of supernatant IL-6 and NO were increased clearly in zinc deficiency group and corticosterone stressed groups.
  
  
  CONCLUSIONS
  Our results suggest that zinc deficiency may decrease while zinc complementarity increase the expressions of metallothioneins and MT-1 mRNA, MT-3 mRNA in stressed hippocampal neurons in vitro.
authors.affiliation: Institute of Health and Environmental Medicine, Academy of Military Medical Sciences, Tianjin 300050, China.
authors.forename: Wei-Qiang
authors.initials: WQ
authors.lastname: Chen
authors.forename: Yi-Yong
authors.initials: YY
authors.lastname: Cheng
authors.forename: Shu-Tian
authors.initials: ST
authors.lastname: Li
authors.forename: Yan
authors.initials: Y
authors.lastname: Hong
authors.forename: Yue
authors.initials: Y
authors.lastname: Hou
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism*; D066298:In Vitro Techniques; D008668:Metallothionein / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D012333:RNA, Messenger; D051381:Rats; D015032:Zinc / Q000494:pharmacology*
chemical_list: D012333:RNA, Messenger; D008668:Metallothionein; D015032:Zinc