title: The protein encoded by NCgl1221 in Corynebacterium glutamicum functions as a mechanosensitive channel.
abstract: The function of the NCgl1221-encoded protein of Corynebacterium glutamicum was analyzed using Bacillus subtilis as host because a method for preparing the giant provacuole required for electrophysiological studies has been established. Expression of NCgl1221 in a strain deficient in mscL and ykuT, both of which encode mechanosensitive channels, resulted in an 8.9-fold higher cell survival rate upon osmotic downshock than the control. Electrophysiological investigation showed that the giant provacuole prepared from this strain, expressing NCgl1221, exhibited significantly higher pressure-dependent conductance than the control. These findings show that the NCgl1221-encoded protein functions as a mechanosensitive channel.
authors.affiliation: Department of Green and Sustainable Chemistry, Tokyo Denki University, Tokyo, Japan.
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Hashimoto
authors.forename: Koji
authors.initials: K
authors.lastname: Nakamura
authors.forename: Teruo
authors.initials: T
authors.lastname: Kuroda
authors.forename: Isamu
authors.initials: I
authors.lastname: Yabe
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Nakamatsu
authors.forename: Hisashi
authors.initials: H
authors.lastname: Kawasaki
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002470:Cell Survival; D048230:Corynebacterium glutamicum / Q000166:cytology / Q000378:metabolism*; D055724:Electrophysiological Phenomena; D004926:Escherichia coli / Q000235:genetics; D007473:Ion Channels / Q000235:genetics / Q000378:metabolism*; D009997:Osmotic Pressure
chemical_list: D001426:Bacterial Proteins; D007473:Ion Channels