title: Increased stromal-cell-derived factor 1 enhances the homing of bone marrow derived mesenchymal stem cells in dilated cardiomyopathy in rats.
abstract: BACKGROUND
  Stem cell transplantation has been shown to have beneficial effects on dilated cardiomyopathy. However, mechanism for stem cell homing to cardiac tissue in dilated cardiomyopathy has not yet been elucidated.
  
  
  METHODS
  Mesenchymal stem cells were obtained from rat bone marrow, expanded in vitro, and labeled with (99m)Tc. Cardiomyopathy model was induced by doxorubicin in rats. (99m)Tc labeled cells were infused into the left ventricles in cardiomyopathy and control rats. Sixteen hours after injection, animals were sacrificed and different tissues were harvested to measure specific radioactivity. By use of real-time polymerase chain reaction and immunohistochemistry, mRNA and protein expressions for stromal-cell-derived factor 1 in cardiac tissue were measured.
  
  
  RESULTS
  Labeling efficiency of mesenchymal stem cells was (70.0 ± 11.2)%. Sixteen hours after mesenchymal stem cell transplantation, the heart-to-muscle radioactivity ratio was increased significantly in cardiomyopathy hearts as compared to control hearts. Both mRNA and protein expressions of stromal-cell-derived factor 1 were up-regulated in cardiomyopathy hearts as compared with control hearts.
  
  
  CONCLUSIONS
  In dilated cardiomyopathy induced by doxorubicin up-regulated expression of stromal-cell-derived factor 1 in heart may induce mesenchymal stem cells home to the heart.
authors.affiliation: Department of Cardiology, First Affiliated Hospital of Nanjing Medical University/Jiangsu Province Hospital, Nanjing, Jiangsu 210029, China.
authors.forename: Yan-Li
authors.initials: YL
authors.lastname: Zhou
authors.forename: Hai-Feng
authors.initials: HF
authors.lastname: Zhang
authors.forename: Xin-Li
authors.initials: XL
authors.lastname: Li
authors.forename: Ruo-Min
authors.initials: RM
authors.lastname: DI
authors.forename: Wen-Ming
authors.initials: WM
authors.lastname: Yao
authors.forename: Dian-Fu
authors.initials: DF
authors.lastname: Li
authors.forename: Jian-Lin
authors.initials: JL
authors.lastname: Feng
authors.forename: Jun
authors.initials: J
authors.lastname: Huang
authors.forename: Ke-Jiang
authors.initials: KJ
authors.lastname: Cao
authors.forename: Michael
authors.initials: M
authors.lastname: Fu
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000378:metabolism; D002311:Cardiomyopathy, Dilated / Q000628:therapy*; D002478:Cells, Cultured; D054377:Chemokine CXCL12 / Q000235:genetics / Q000378:metabolism*; D007150:Immunohistochemistry; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D054377:Chemokine CXCL12