title: Three-dimensional depth-resolved and extended-resolution micro-particle characterization by holographic light scattering spectroscopy.
abstract: Fourier-holographic light scattering spectroscopy is applied to record complex angular scattering spectra of two- and three-dimensional samples over a wide field of view. We introduce a computational depth sectioning technique and, for the first time, demonstrate that a single-exposure hologram can generate a quantitative, three-dimensional map of particle sizes and locations over several cubic millimeters with micrometer resolution. Such spatially resolved maps of particle sizes are generated by Mie-inversion and could not be ascertained from the directly reconstructed intensity-distribution images. We also demonstrate synthesis of multiple angular scattering intensity spectra to increase the angular range and improve size detection sensitivity.
authors.affiliation: Optical + Biomedical Engineering Laboratory, School of Electrical, Electronic & Computer Engineering, M018, The University of Western Australia, 35 Stirling Highway, Crawley, Western Australia 6009, Australia. tgutzler@ee.uwa.edu.au
authors.forename: Thomas
authors.initials: T
authors.lastname: Gutzler
authors.forename: Timothy R
authors.initials: TR
authors.lastname: Hillman
authors.forename: Sergey A
authors.initials: SA
authors.lastname: Alexandrov
authors.forename: David D
authors.initials: DD
authors.lastname: Sampson
mesh_terms: D006696:Holography / Q000379:methods*; D007089:Image Enhancement / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008027:Light; D008863:Microspheres; D010316:Particle Size; D012542:Scattering, Radiation; D013057:Spectrum Analysis / Q000379:methods*