title: [Comparisons of several methods used to isolate outer hair cells and morphological observations in cochlea].
abstract: OBJECTIVE
  The comparison of several methods which were used to isolate cochlear outer hair cells and the observations of morphology were researched.
  
  
  METHODS
  Three different separating methods of outer hair cells in cochlea were adopted; the morphology of outer hair cells in cochlea and the morphology of cochlear stretched preparation in Silver Nitrate staining were also investigated.
  
  
  RESULTS
  Single alive OHC in cochlea was disassociated by all methods, with microscope and cochlear stretched preparation's staining. We could also observe appearances and distributions of OHC in cochlea.
  
  
  CONCLUSIONS
  It is successful to isolate single alive cochlear OHC, and it will be very important to investigate deeply normal physiological functions and changes of functions and morphology in some pathologic status in cochlear OHCs.
authors.affiliation: Department of Physiology, Fundamental School of LiaoNing Medical College, Jinzhou 121001, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Tian
authors.forename: Li
authors.initials: L
authors.lastname: Yu
mesh_terms: D000818:Animals; D002469:Cell Separation / Q000379:methods*; D003051:Cochlea / Q000166:cytology / Q000502:physiology; D005260:Female; D006168:Guinea Pigs; D018072:Hair Cells, Auditory, Outer / Q000166:cytology* / Q000502:physiology; D008297:Male; D016622:Silver Staining