title: Ordered mesoporous polymer-silica hybrid nanoparticles as vehicles for the intracellular controlled release of macromolecules.
abstract: A two-dimensional hexagonal ordered mesoporous polymer-silica hybrid nanoparticle (PSN) material was synthesized by polymerization of acrylate monomers on the surface of SBA-15 mesoporous silica nanoparticles. The structure of the PSN material was analyzed using a series of different techniques, including transmission electron microscopy, powder X-ray diffraction, and N(2) sorption analysis. These structurally ordered mesoporous polymer-silica hybrid nanoparticles were used for the controlled release of membrane-impermeable macromolecules inside eukaryotic cells. The cellular uptake efficiency and biocompatibility of PSN with human cervical cancer cells (HeLa) were investigated. Our results show that the inhibitory concentration (IC(50)) of PSN is very high (>100 μg/mL per million cells), while the median effective concentration for the uptake (EC(50)) of PSN is low (EC(50) = 4.4 μg/mL), indicating that PSNs are fairly biocompatible and easily up-taken in vitro. A membrane-impermeable macromolecule, 40 kDa FITC-Dextran, was loaded into the mesopores of PSNs at low pH. We demonstrated that the PSN material could indeed serve as a transmembrane carrier for the controlled release of FITC-Dextran at the pH level inside live HeLa cells. We believe that further developments of this PSN material will lead to a new generation of nanodevices for intracellular controlled delivery applications.
authors.affiliation: Green Chemistry Research Division, Korea Research Institute of Chemical Technology, P.O. Box 107, Sinseongro 19, Yuseong-gu, Daejeon 305-600, Korea. twkim@krict.re.kr
authors.forename: Tae-Wan
authors.initials: TW
authors.lastname: Kim
authors.forename: Igor I
authors.initials: II
authors.lastname: Slowing
authors.forename: Po-Wen
authors.initials: PW
authors.lastname: Chung
authors.forename: Victor Shang-Yi
authors.initials: VS
authors.lastname: Lin
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry* / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D003692:Delayed-Action Preparations; D003911:Dextrans / Q000378:metabolism*; D004337:Drug Carriers / Q000737:chemistry* / Q000378:metabolism; D004705:Endocytosis; D016650:Fluorescein-5-isothiocyanate / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D042541:Intracellular Space / Q000378:metabolism*; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D058105:Polymerization; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D003692:Delayed-Action Preparations; D003911:Dextrans; D004337:Drug Carriers; C509969:SBA-15; D012822:Silicon Dioxide; D016650:Fluorescein-5-isothiocyanate