title: Stand-alone self-powered integrated microfluidic blood analysis system (SIMBAS).
abstract: We present a self-powered integrated microfluidic blood analysis system (SIMBAS) that does not require any external connections, tethers, or tubing to deliver and analyze a raw whole-blood sample. SIMBAS only requires the user to place a 5 μL droplet of whole-blood at the inlet port of the device, whereupon the stand-alone SIMBAS performs on-chip removal of red and white cells, without external valving or pumping mechanisms, followed by analyte detection in platelet-containing plasma. Five complete biotin-streptavidin sample-to-answer assays are performed in 10 min; the limit of detection is 1.5 pM. Red and white blood cells are removed by trapping them in an integral trench structure. Simulations and experimental data show 99.9% to 100% blood cell retention in the passive structure. Powered by pre-evacuation of its PDMS substrate, SIMBAS' guiding design principle is the integration of the minimal number of components without sacrificing effectiveness in performing rapid complete bioassays, a critical step towards point-of-care molecular diagnostics.
authors.affiliation: Biomedical Diagnostics Institute, National Centre for Sensor Research, Dublin City University, Glasnevin, Dublin 9, Ireland.
authors.forename: Ivan K
authors.initials: IK
authors.lastname: Dimov
authors.forename: Lourdes
authors.initials: L
authors.lastname: Basabe-Desmonts
authors.forename: Jose L
authors.initials: JL
authors.lastname: Garcia-Cordero
authors.forename: Benjamin M
authors.initials: BM
authors.lastname: Ross
authors.forename: Younggeun
authors.initials: Y
authors.lastname: Park
authors.forename: Antonio J
authors.initials: AJ
authors.lastname: Ricco
authors.forename: Luke P
authors.initials: LP
authors.lastname: Lee
mesh_terms: D001773:Blood Cells / Q000166:cytology; D002469:Cell Separation; D006403:Hematologic Tests / Q000295:instrumentation*; D006801:Humans; D007118:Immunoassay; D007700:Kinetics; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D010949:Plasma / Q000166:cytology; D019095:Point-of-Care Systems; D018511:Systems Integration