title: Reg IV protein and mRNA expression in different rat organs.
abstract: The Reg IV gene has been documented in the human colon, small intestine, stomach and pancreas. Expression of the Reg IV in different cell types has been associated with regeneration, cell growth and cell survival, cell adhesion and resistance to apoptosis. Since the distribution of the Reg IV protein in normal rat tissues is unknown, the aim of this study was to reveal the expression of the Reg IV protein in structurally and functionally different rat organs. The expression of Reg IV gene was analyzed by Western blot and reverse transcription-polymerase chain reaction. Immunohistochemistry was used to localize Reg IV protein. Reg IV protein was expressed in pancreas, stomach, small intestine, colon, brain, spleen, kidney and urinary bladder in two-month-old male Wistar rats. In addition, the expression of Reg IV mRNA by reverse transcription-polymerase chain reaction was confirmed. Our study provides detailed information about the expression and localization of Reg IV protein in different rat organs. These findings provide an evidence of Reg IV expression in different rat organs, which may help elucidate a potential role in growth and proliferation of different cells like other members of the Reg family genes which act as growth factors in the different organs.
authors.affiliation: Department of Anesthesiology and Intensive Care, University Hospital Rijeka, Kresimirova 42, Rijeka, Croatia.
authors.forename: Josip
authors.initials: J
authors.lastname: Azman
authors.forename: Gordana
authors.initials: G
authors.lastname: Starcevic Klasan
authors.forename: Danijel
authors.initials: D
authors.lastname: Ivanac
authors.forename: Anne
authors.initials: A
authors.lastname: Picard
authors.forename: Dubravka
authors.initials: D
authors.lastname: Jurisic-Erzen
authors.forename: Marina
authors.initials: M
authors.lastname: Nikolic
authors.forename: Daniela
authors.initials: D
authors.lastname: Malnar
authors.forename: Juraj
authors.initials: J
authors.lastname: Arbanas
authors.forename: Romana
authors.initials: R
authors.lastname: Jerkovic
mesh_terms: D000818:Animals; D000951:Antigens, Neoplasm / Q000096:biosynthesis*; D014408:Biomarkers, Tumor / Q000096:biosynthesis*; D049109:Cell Proliferation; D005786:Gene Expression Regulation; D007150:Immunohistochemistry; D037181:Lectins, C-Type / Q000096:biosynthesis*; D008297:Male; D009928:Organ Specificity; D000073718:Pancreatitis-Associated Proteins; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D037181:Lectins, C-Type; D000073718:Pancreatitis-Associated Proteins; C000612141:REG3A protein, human; D012333:RNA, Messenger