title: A highly sensitive resonance scattering spectral assay for carcinoembryonic antigen using immunonanogold as probe and nanocatalyst of NH₂(OH-Cu(II) particle reaction.
abstract: Nanogold of 10 nm was used to label carcinoembryonic antigen antibody (CEAAb) to prepare a probe (Au-CEAAb) for carcinoembryonic antigen (CEA). In a Na₂HPO₄-NaH₂PO₄ buffer solution of pH 6.8, CEA reacted with Au-CEAAb to form a big Au-CEAAb-CEA immunocomplex that can be removed by centrifugation. The unreacted Au-CEAAb in the centrifugal supernatant exhibited catalytic effect on the Cu₂O particle reaction, and the Cu₂O particles displayed a resonance scattering (RS) peak at 602 nm. When CEA increased, the RS intensity at 602 nm decreased, and the decreased RS intensity (ΔI (602 nm)) was linear to CEA concentration (C (CEA)) in the range of 0.02-12 ng mL(-1), with the regression equation of ΔI (602 nm) = 27.1 C (CEA) + 3.3, correlation coefficient of 0.9978 and detection limit of 3 pg mL(-1) CEA. The proposed method was applied to detect CEA in real samples, with satisfactory results.
authors.affiliation: Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection of Education Ministry, Guangxi Key Laboratory of Environmental Engineering and Protection Assessment, Guangxi Normal University, Guilin, China.
authors.forename: Qingye
authors.initials: Q
authors.lastname: Liu
authors.forename: Guiqing
authors.initials: G
authors.lastname: Wen
authors.forename: Aihui
authors.initials: A
authors.lastname: Liang
authors.forename: Tingsheng
authors.initials: T
authors.lastname: Li
authors.forename: Zhiliang
authors.initials: Z
authors.lastname: Jiang
mesh_terms: D001681:Biological Assay; D002272:Carcinoembryonic Antigen / Q000032:analysis*; D002384:Catalysis; D003300:Copper / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D019811:Hydroxylamine / Q000737:chemistry; D007124:Immunoenzyme Techniques / Q000379:methods; D007150:Immunohistochemistry / Q000379:methods*; D036103:Nanotechnology / Q000379:methods*; D010100:Oxygen / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D012044:Regression Analysis; D012542:Scattering, Radiation
chemical_list: D002272:Carcinoembryonic Antigen; D019811:Hydroxylamine; D011092:Polyethylene Glycols; D003300:Copper; D010100:Oxygen