title: Imaging and measuring the rituximab-induced changes of mechanical properties in B-lymphoma cells using atomic force microscopy.
abstract: The topography and mechanical properties of single B-lymphoma cells have been investigated by atomic force microscopy (AFM). With the assistance of microfabricated patterned pillars, the surface topography and ultrastructure of single living B-lymphoma cell were visualized by AFM. The apoptosis of B-lymphoma cells induced by rituximab alone was observed by acridine orange/ethidium bromide (AO/EB) double fluorescent staining. The rituximab-induced changes of mechanical properties in B-lymphoma cells were measured dynamically and the results showed that B-lymphoma cells became dramatically softer after incubation with rituximab. These results can improve our understanding of rituximab'effect and will facilitate the further investigation of the underlying mechanisms.
authors.affiliation: State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang 110016, China.
authors.forename: Mi
authors.initials: M
authors.lastname: Li
authors.forename: Lianqing
authors.initials: L
authors.lastname: Liu
authors.forename: Ning
authors.initials: N
authors.lastname: Xi
authors.forename: Yuechao
authors.initials: Y
authors.lastname: Wang
authors.forename: Zaili
authors.initials: Z
authors.lastname: Dong
authors.forename: Osamu
authors.initials: O
authors.lastname: Tabata
authors.forename: Xiubin
authors.initials: X
authors.lastname: Xiao
authors.forename: Weijing
authors.initials: W
authors.lastname: Zhang
mesh_terms: D058846:Antibodies, Monoclonal, Murine-Derived / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000000981:diagnostic imaging / Q000187:drug effects; D006801:Humans; D016393:Lymphoma, B-Cell / Q000648:ultrastructure*; D018625:Microscopy, Atomic Force; D000069283:Rituximab; D014463:Ultrasonography
chemical_list: D058846:Antibodies, Monoclonal, Murine-Derived; D000970:Antineoplastic Agents; D000069283:Rituximab