title: Novel SNPs of the bovine PRLR gene associated with milk production traits.
abstract: The single nucleotide polymorphisms (SNPs) within exon 10 of the prolactin receptor gene (PRLR) were detected in Chinese Holstein cows using polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) and DNA sequencing methods, and their genetic effects on milk production traits were evaluated in this study. Two newly detected SNPs (g.9206G→A and g.9681C→T) caused amino acid variations E378K and A536V, respectively, which were then preliminarily predicted at the topological level. Statistical results indicated that the two SNPs were significantly associated with milk yields, and cows with the combined genotype GGCC showed superior milk performance. A putative phosphorylation site was identified at residue 378K ([ST]-×-[RK]), which offers a partial explanation for the associations. These results suggest that the two novel SNPs within exon 10 of the PRLR gene associated with milk production traits are useful genetic markers in a selection program for Holstein dairy cattle.
authors.affiliation: School of Life Sciences, Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, Xuzhou Normal University, Xuzhou, China. lajand@126.com
authors.forename: Aijun
authors.initials: A
authors.lastname: Lü
authors.forename: Xiucai
authors.initials: X
authors.lastname: Hu
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
authors.forename: Yongjun
authors.initials: Y
authors.lastname: Dong
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xiuqin
authors.initials: X
authors.lastname: Wang
mesh_terms: D000818:Animals; D001483:Base Sequence; D002417:Cattle; D005260:Female; D040461:Gene Components; D056726:Genetic Association Studies; D014644:Genetic Variation; D006239:Haplotypes; D007774:Lactation / Q000235:genetics*; D008892:Milk / Q000737:chemistry; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D017434:Protein Structure, Tertiary; D011981:Receptors, Prolactin / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D011981:Receptors, Prolactin