title: [Effects of genistein on bone mineralization in ovariectomized rats].
abstract: OBJECTIVE
  To investigate the effects of genistein on bone mineralization in ovariectomized rats.
  
  
  METHODS
  Forty-seven Wistar rats were randomly allocated into six groups: sham-operated (sham), ovariectomized (ovx), ovariectomized supplied with diethyl stilbestrol (E, 20 microg x kg bw(-1) x d(-1)) or genistein (25, 50, 100 mg x kg bw(-1) x d(-1)). After the rats had been fed for three months, analysis of the bone mineral density, parameters related to mineralization, bone content of Ca, P, Mg, Mn and Zn and serum concentration of parathyroid calcitonin and estrogen was performed.
  
  
  RESULTS
  Bone mineral density, bone Ca, P, Zn and Mg content and serum estrogen concentration in ovariectomized rats were significantly decreased, but mean osteoid width increased, mineralization lag time and osteoid maturation period prolonged compared with sham animals. After three months supplementation to ovariectomized rats, bone Ca, P and Mg content increased, mean osteoid width decreased, mineralization lag time and osteoid maturation period shortened compared with ovariectomized animals.
  
  
  CONCLUSIONS
  Genistein promotes bone mineralization by increasing bone Ca, P, Mg and adjusting serum calcitonin to prevent osteoporosis.
authors.affiliation: Department of Nutrition, General Hospital of Chinese PLA, Beijing 100853, China.
authors.forename: Yue-hong
authors.initials: YH
authors.lastname: Zhang
authors.forename: Hong
authors.initials: H
authors.lastname: Jin
authors.forename: Zhi-qin
authors.initials: ZQ
authors.lastname: Xu
authors.forename: Wen-kao
authors.initials: WK
authors.lastname: Nan
authors.forename: Xian-yuan
authors.initials: XY
authors.lastname: Wang
authors.forename: Chang-yong
authors.initials: CY
authors.lastname: Xue
authors.forename: Lan-xing
authors.initials: LX
authors.lastname: Gao
mesh_terms: D000818:Animals; D015519:Bone Density / Q000187:drug effects*; D001862:Bone Resorption; D002113:Calcification, Physiologic / Q000187:drug effects*; D005260:Female; D019833:Genistein / Q000494:pharmacology*; D010052:Ovariectomy; D051381:Rats; D017208:Rats, Wistar
chemical_list: D019833:Genistein