title: Mutation of conserved tryptophan residues at the dimer interface of Staphylococcus aureus nitric oxide synthase.
abstract: Nitric oxide synthases (NOSs) share two invariant tryptophan residues within a conserved helical lariat that is part of the pterin-binding site and dimer interface. We mutated Staphylococcus aureus NOS Trp-314 (to alanine, phenylalanine, tyrosine and histidine) and Trp-316 (to alanine, phenylalanine and tyrosine) and characterized the effects of mutation on heme environment, quaternary structure, enzymatic activity, and substrate affinity. With arginine present, all saNOS variants bound heme with native thiolate ligation, formed high spin ferric complexes and were dimeric. All variants catalyze the peroxide-dependent oxidation of N-hydroxy-l-arginine, at rates from 10% to 55% of wild type activity. Arginine-free proteins are dimeric with the exception of W314A. Arginine affinity for all variants decreases with increasing temperature between 15 and 42 °C but is precipitous for position-314 variants. Previous structural and biophysical characterization of NOS oxygenase domains demonstrated that the protein can exist in either a tight or loose conformation, with the former corresponding to the active state of the protein. In the position-314 variants it is likely that the loose conformation is favoured, owing to the loss of a hydrogen bond between the indole side chain and the polypeptide backbone of the helical lariat.
authors.affiliation: Departments of Chemistry and Biology, Trent University, Peterborough, ON K9J7B8, Canada.
authors.forename: Daniel Ben
authors.initials: DB
authors.lastname: Lustig
authors.forename: Cora
authors.initials: C
authors.lastname: Kempt
authors.forename: Samiah
authors.initials: S
authors.lastname: Alam
authors.forename: James
authors.initials: J
authors.lastname: Clancy
authors.forename: Janet
authors.initials: J
authors.lastname: Yee
authors.forename: Steven Patrick
authors.initials: SP
authors.lastname: Rafferty
mesh_terms: D019943:Amino Acid Substitution; D001120:Arginine / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D020134:Catalytic Domain; D017124:Conserved Sequence; D019281:Dimerization; D004789:Enzyme Activation; D007700:Kinetics; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D019001:Nitric Oxide Synthase / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D054730:Protein Interaction Domains and Motifs; D020836:Protein Structure, Quaternary; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013053:Spectrophotometry; D013211:Staphylococcus aureus / Q000201:enzymology* / Q000235:genetics*; D013379:Substrate Specificity; D014364:Tryptophan / Q000737:chemistry
chemical_list: D001426:Bacterial Proteins; D011994:Recombinant Proteins; D014364:Tryptophan; D001120:Arginine; D019001:Nitric Oxide Synthase