title: The genomes of four novel begomoviruses and a new Sida micrantha mosaic virus strain from Bolivian weeds.
abstract: Begomovirus is the largest genus within the family Geminiviridae and includes economically important plant DNA viruses infecting a broad range of plant species and causing devastating crop diseases, mainly in subtropical and tropical countries. Besides cultivated plants, many weeds act as virus reservoirs. Eight begomovirus isolates from Bolivian weeds were examined using rolling-circle amplification (RCA) and restriction fragment length polymorphism (RFLP). An efficient, novel cloning strategy using limited Sau3A digestion to obtain tandem-repeat inserts allowed the sequencing of the complete genomes. The viruses were classified by phylogenetic analysis as typical bipartite New World begomoviruses. Four of them represented distinct new virus species, for which the names Solanum mosaic Bolivia virus, Sida mosaic Bolivia virus 1, Sida mosaic Bolivia virus 2, and Abutilon mosaic Bolivia virus are proposed. Three were variants of a new strain of Sida micrantha mosaic virus (SimMV), SimMV-rho[BoVi07], SimMV-rho[Bo:CF1:07] and SimMV-rho[Bo:CF2:07], and one was a new variant of a previously described SimMV, SimMV-MGS2:07-Bo.
authors.affiliation: Department of Molecular Biology and Plant Virology, Institute of Biology, University of Stuttgart, Pfaffenwaldring 57, Stuttgart, Germany.
authors.forename: Patrícia Soares
authors.initials: PS
authors.lastname: Wyant
authors.forename: Diether
authors.initials: D
authors.lastname: Gotthardt
authors.forename: Benjamin
authors.initials: B
authors.lastname: Schäfer
authors.forename: Björn
authors.initials: B
authors.lastname: Krenz
authors.forename: Holger
authors.initials: H
authors.lastname: Jeske
mesh_terms: D053839:Begomovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D001838:Bolivia; D004279:DNA, Viral / Q000235:genetics; D016679:Genome, Viral; D019660:Malvaceae / Q000821:virology*; D009029:Mosaic Viruses / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology; D032322:Solanum / Q000821:virology
chemical_list: D004279:DNA, Viral