title: Effect of bioregulators isolated from the liver, blood serum, and bile of mammals on the state of newt liver tissue in organotypic culture.
abstract: We developed models of in vitro organotypic culturing of newt liver tissue with and without adhesion to the substrate. The effects of bioregulators isolated from mammalian liver, blood serum, and bile were studied on the developed models and their specificity was demonstrated. The state of the liver was evaluated by the area of clusters of pigmented cells and by the number of mitoses in the connective tissue cells of the cortical layer. These bioregulators exhibited their biological effects only under conditions of roller organotypic culturing of newt liver tissue.
authors.affiliation: N. K. Kol'tsov Institute of Developmental Biology, Russian Academy of Sciences; A. N. Nesmeyanov Institute of Elementorganic Compounds, Russian Academy of Sciences, Moscow, Russia.
authors.forename: V P
authors.initials: VP
authors.lastname: Yamskova
authors.forename: A V
authors.initials: AV
authors.lastname: Borisenko
authors.forename: M S
authors.initials: MS
authors.lastname: Krasnov
authors.forename: A P
authors.initials: AP
authors.lastname: Il'ina
authors.forename: E Yu
authors.initials: EY
authors.lastname: Rybakova
authors.forename: D I
authors.initials: DI
authors.lastname: Malcev
authors.forename: I A
authors.initials: IA
authors.lastname: Yamskov
mesh_terms: D000818:Animals; D001646:Bile / Q000737:chemistry*; D006133:Growth Substances / Q000097:blood / Q000302:isolation & purification* / Q000494:pharmacology*; D008099:Liver / Q000737:chemistry* / Q000166:cytology*; D009924:Organ Culture Techniques / Q000379:methods*; D012455:Salamandridae
chemical_list: D006133:Growth Substances