title: Determination of paraneoplastic autoimmune responses by tumor cell biology and intratumoral IFN-alpha/IL-12 in breast cancer patients.
abstract: A wide variety of cancer types has been associated with paraneoplastic autoimmune disorders and with the induction of autoimmunity against several autoantigens, among them self-antigens that are also expressed by tumor cells. This raises the question of autoimmune disorders as a result of immune reactions to the tumor. To date, however, requirements for the generation of autoimmune reactions in cancer patients remain largely unclear. In this study, we characterized conditions in altogether 131 patients, which determine autoimmune responses in primary breast cancer patients. We used ex vivo IFN-γ EliSpot assays against autologous tumor or skin lysates to evaluate tumor- and auto-reactive T-cells (TCs) in the bone marrow (BM) as well as ELISA, ECLIA, and turbidimetric immunoassays for the detection of auto-reactive antibodies in the peripheral blood and compared results to intratumoral cytokine concentrations and pathobiological features of the primary tumor tissue. We here demonstrate a significant correlation between anti-tumor BMTC responses and cellular autoimmune reactivity in primary breast cancer patients (P = 0.002). Humoral autoimmune reactions, however, were negatively correlated with anti-tumor TC immunity (P = 0.039). We observed auto-reactive BMTCs especially in patients with well-differentiated, hormone receptor-positive carcinomas (P = 0.009). Furthermore, elevated concentrations of intratumoral IFN-α significantly correlated with the induction of cellular autoimmune reactivity (P = 0.0002), while humoral autoimmune reactions correlated with increased levels of intratumoral IL-12 (P = 0.04). Altogether, these data indicate a significant role of the tumor microenvironment and particularly that of IFN-α and IL-12 in the induction of systemic autoimmune responses and imply that the primary tumor tissue represents an integral site of autoimmune regulation in cancer patients.
authors.affiliation: Department of Gynecology and Obstetrics, University Hospital of Heidelberg, Voßstraße 9, 69115 Heidelberg, Germany. christoph.domschke@med.uni-heidelberg.de
authors.forename: Christoph
authors.initials: C
authors.lastname: Domschke
authors.forename: Florian
authors.initials: F
authors.lastname: Schuetz
authors.forename: Yingzi
authors.initials: Y
authors.lastname: Ge
authors.forename: Joachim
authors.initials: J
authors.lastname: Rom
authors.forename: Markus
authors.initials: M
authors.lastname: Zorn
authors.forename: Hans-Peter
authors.initials: HP
authors.lastname: Sinn
authors.forename: Frederik
authors.initials: F
authors.lastname: Marmé
authors.forename: Sarah
authors.initials: S
authors.lastname: Schott
authors.forename: Joerg
authors.initials: J
authors.lastname: Heil
authors.forename: Alexander
authors.initials: A
authors.lastname: Scharf
authors.forename: Christof
authors.initials: C
authors.lastname: Sohn
authors.forename: Andreas
authors.initials: A
authors.lastname: Schneeweiss
authors.forename: Philipp
authors.initials: P
authors.lastname: Beckhove
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001323:Autoantibodies / Q000097:blood; D015551:Autoimmunity; D001853:Bone Marrow / Q000276:immunology; D001943:Breast Neoplasms / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D016898:Interferon-alpha / Q000276:immunology*; D018664:Interleukin-12 / Q000276:immunology*; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008875:Middle Aged; D010257:Paraneoplastic Syndromes / Q000276:immunology*
chemical_list: D001323:Autoantibodies; D016898:Interferon-alpha; D018664:Interleukin-12