title: MicroRNA-206 is associated with invasion and metastasis of lung cancer.
abstract: MicroRNAs are novel small noncoding RNA molecules that regulate gene expression at the post-transcriptional level. Compelling evidence reveals that there is a causative link between microRNAs deregulation and cancer development and progression. The present study aims to explore the function of miR-206 in the proliferation, apoptosis, motility, and invasion of nonsmall cell lung cancer. Using real-time PCR, we detected the miR-206 expression of normal lung tissues, tumor tissues, human normal bronchial epithelial cell line, and six lung cancer cell lines (LCCLs). Then, we evaluated the role of miR-206 in cell proliferation, apoptosis, and invasion using Cell Counting Kit-8 assay, Annexin-V/FITC assay, wound healing, and Transwell assay in LCCLs. As a result, miR-206 expression level was lower in high metastasis tumors and 95D than low metastasis tumors and normal lung tissues as well as other LCCLs. After miR-206 was upregulated in LCCLs, cell proliferation was notably attenuated and apoptosis was significantly increased. Furthermore, overexpression of miR-206 inhibited migration and invasion of lung cancer cells. In conclusion, our data suggest that expression level of miR-206 was inversely correlated with metastatic potential of lung cancer.
authors.affiliation: Department of Thoraco-Cardiac Surgery, The First Affiliated Hospital of Soochow University, Suzhou, China.
authors.forename: Xiaochen
authors.initials: X
authors.lastname: Wang
authors.forename: Chunhua
authors.initials: C
authors.lastname: Ling
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Bai
authors.forename: Jun
authors.initials: J
authors.lastname: Zhao
mesh_terms: D002460:Cell Line; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism* / Q000473:pathology*; D035683:MicroRNAs / Q000009:adverse effects / Q000096:biosynthesis / Q000502:physiology*; D009361:Neoplasm Invasiveness / Q000473:pathology
chemical_list: C546087:MIRN206 microRNA, human; D035683:MicroRNAs