title: [The effect of ginkgolide B on action potential, L-type calcium current and delayed rectifier potassium current in ischemic guinea pig ventricular myocytes].
abstract: OBJECTIVE
  To study the effect of ginkgolide B from Ginkgo leave on action potential (AP), L-type calcium current (I(Ca) - L) and delayed rectifier potassium current (I(K)) in normal and ischemic guinea pig ventricular myocytes.
  
  
  METHODS
  With the standard microelectrode technique to record action potential and whole-cell variant patch-clamp technique to record calcium and potassium current.
  
  
  RESULTS
  (1) Under normal condition, ginkgolide B shortened APD and had no effect on RP, AP and V(max). Ginkgolide B also increased I(K) in a concentration dependent manner and had no significant effect on I(Ca) - L (2) Under ischemia condition, it was observed that shortening of APD, APA, decrease V(max) and depolarization of RP was induced by ischemia, but ginkgolide B could attenuate above--mentioned changes. (3) Under ischemia condition, I(Ca) - L and I(K) were inhibited, perfusion with ischemia solution containing ginkgolide B could reverse the decrease of I(Ca) - L and I(K).
  
  
  CONCLUSIONS
  Ginkgolide B had protective effect on ischemic myocardium to prevent ischemic arrhythmia.
authors.affiliation: Department of Physiology, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China.
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Qi
authors.forename: Zhi-Xiong
authors.initials: ZX
authors.lastname: Zhang
authors.forename: Qi-Qi
authors.initials: QQ
authors.lastname: Cui
authors.forename: Wei-Bin
authors.initials: WB
authors.lastname: Shi
authors.forename: You-Qiu
authors.initials: YQ
authors.lastname: Xu
mesh_terms: D000200:Action Potentials / Q000187:drug effects*; D000818:Animals; D020746:Calcium Channels, L-Type / Q000187:drug effects; D051668:Delayed Rectifier Potassium Channels / Q000187:drug effects; D046934:Ginkgolides / Q000494:pharmacology*; D006168:Guinea Pigs; D006352:Heart Ventricles / Q000187:drug effects; D007783:Lactones / Q000494:pharmacology*; D017202:Myocardial Ischemia / Q000378:metabolism*; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000378:metabolism; D018408:Patch-Clamp Techniques
chemical_list: D020746:Calcium Channels, L-Type; D051668:Delayed Rectifier Potassium Channels; D046934:Ginkgolides; D007783:Lactones; C045856:ginkgolide B