title: Angiogenic activity of dentin matrix components.
abstract: Angiogenesis is key to both the development and regeneration of the dentin-pulp complex.
  
  
  OBJECTIVE
  We hypothesized that proangiogenic signaling molecules sequestered in dentin matrix can be solubilised to induce angiogenic events.
  
  
  METHODS
  Matrix components were extracted from powdered sound human dentin with EDTA and their dose-dependent (0.0001-5 mg/mL) effects examined in endothelial cells in an in vitro angiogenic tube formation assay, proliferation assay, and transcriptional regulation of the VEGF and VEGF-R2 genes.
  
  
  RESULTS
  Lower concentrations of dentin matrix components were found to show proangiogenic activity, whereas higher concentrations suppressed angiogenic activity.
  
  
  CONCLUSIONS
  This study highlights that the release of dentin matrix components after dental injury can contribute to the angiogenic events that support pulp regeneration.
authors.affiliation: Department of Oral Biology, School of Dentistry, University of Birmingham, Birmingham, UK.
authors.forename: Rong
authors.initials: R
authors.lastname: Zhang
authors.forename: Paul R
authors.initials: PR
authors.lastname: Cooper
authors.forename: Gay
authors.initials: G
authors.lastname: Smith
authors.forename: Jacques E
authors.initials: JE
authors.lastname: Nör
authors.forename: Anthony J
authors.initials: AJ
authors.lastname: Smith
mesh_terms: D042501:Angiogenic Proteins / Q000008:administration & dosage / Q000502:physiology*; D002478:Cells, Cultured; D003804:Dentin / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D042783:Endothelial Cells / Q000187:drug effects / Q000502:physiology*; D016326:Extracellular Matrix Proteins / Q000008:administration & dosage / Q000502:physiology*; D006801:Humans; D018919:Neovascularization, Physiologic / Q000187:drug effects / Q000502:physiology*; D018709:Statistics, Nonparametric
chemical_list: D042501:Angiogenic Proteins; D016326:Extracellular Matrix Proteins