title: [Effects of human HSF1 on transcriptional regulation of prohibitin promoter].
abstract: OBJECTIVE
  To assay the transcriptional regulation effect of hHSF1 on prohibitin gene promoter.
  
  
  METHODS
  The total length of hHSF1 exon was amplified by PCR method and cloned into pcDNA3. 1(+) vector. pcDNA3. 1(+)-hHSF1 and pGI3 prohibitin were co-transfected into HEK293 cells. The luciferase activity was detected by Dual-Luciferase Reporter Assay System to evaluate the transcriptional regulation effect of hHSF1 on prohibitin gene promoter.
  
  
  RESULTS
  The pcDNA3.1(+)-hHSF1 vector was constructed successfully. The assay of luciferase activity showed that the transcription of pGL3-prohibitin was dramatically upregulated by hHSF1.
  
  
  CONCLUSIONS
  hHSF1 can transcriptionally regulate prohibitin expression.
authors.affiliation: Department of Stress Medicine, Institute of Health & Environmental Medicine, Tianjin 300050, China.
authors.forename: Xiao-hua
authors.initials: XH
authors.lastname: Liu
authors.forename: Dong-sheng
authors.initials: DS
authors.lastname: Guo
authors.forename: Xin-xing
authors.initials: XX
authors.lastname: Wang
authors.forename: Zhi-qing
authors.initials: ZQ
authors.lastname: Zhang
authors.forename: Rui
authors.initials: R
authors.lastname: Zhan
authors.forename: Xiao-ming
authors.initials: XM
authors.lastname: Wang
authors.forename: Ling-jia
authors.initials: LJ
authors.lastname: Qian
mesh_terms: D001483:Base Sequence; D004268:DNA-Binding Proteins / Q000502:physiology*; D005786:Gene Expression Regulation; D057809:HEK293 Cells; D000076249:Heat Shock Transcription Factors; D006801:Humans; D008969:Molecular Sequence Data; D000091402:Prohibitins; D011401:Promoter Regions, Genetic; D012097:Repressor Proteins / Q000235:genetics*; D014157:Transcription Factors / Q000502:physiology*; D014158:Transcription, Genetic; D014162:Transfection
chemical_list: D004268:DNA-Binding Proteins; C000619706:HSF1 protein, human; D000076249:Heat Shock Transcription Factors; D000091402:Prohibitins; D012097:Repressor Proteins; D014157:Transcription Factors