title: [Screening and identification of a novel ovarian specific protein in mouse].
abstract: OBJECTIVE
  To screen and identify the novel ovarian specific proteins to explore the possible mechanisms in the oogenesis and early embryo development.
  
  
  METHODS
  The oocytes of BALB/C mice were collected for 2-D electrophoresis. After reaction with anti-citrulline antibody, three positive spots were selected for mass spectrometry. Based on the biologic informatic analysis, a novel peptide sequence was found and specific primers were designed for cloning. A full-length cDNA was identified by reverse transcription and rapid amplification of cDNA ends-polymerase chain reaction (RACE-PCR). Its nucleotide and possible protein sequences were compared with those in the gene bank. And the tissue-specific characteristic of MOP53 was analyzed by Northern blot.
  
  
  RESULTS
  A novel peptide sequence was found in murine oocytes, it came from a predicted protein with 330 amino acids. The predicted protein had ∼ 53 400 and pI 8.05 with conserved WD40, F-box motif. A full-length cDNA sequence with 1510 bp was cloned and named MOP53. Located at chromosome 9 F2, this new gene was specifically expressed in ovary.
  
  
  CONCLUSIONS
  An ovarian specific protein is found. It may be involved in the process of oogenesis and early embryo development based on its biologic informatic characteristics.
authors.affiliation: Department of Obstetrics & Gynecology, Southwest Hospital, Third Military Medical University, Chongqing 400038, China. anyhewei@yahoo.com.cn
authors.forename: Wei
authors.initials: W
authors.lastname: He
authors.forename: Li-hua
authors.initials: LH
authors.lastname: Yang
authors.forename: Li-li
authors.initials: LL
authors.lastname: Sun
authors.forename: Yun-long
authors.initials: YL
authors.lastname: Xiang
authors.forename: Ling
authors.initials: L
authors.lastname: Long
authors.forename: Yu-zhang
authors.initials: YZ
authors.lastname: Wu
mesh_terms: D000818:Animals; D001483:Base Sequence; D004527:Egg Proteins / Q000235:genetics* / Q000302:isolation & purification; D047108:Embryonic Development; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D009866:Oogenesis / Q000235:genetics; D006080:Ovarian Follicle / Q000737:chemistry* / Q000254:growth & development*; D010053:Ovary / Q000737:chemistry* / Q000166:cytology
chemical_list: D004527:Egg Proteins