title: A polycationic antimicrobial and biocompatible hydrogel with microbe membrane suctioning ability.
abstract: Despite advanced sterilization and aseptic techniques, infections associated with medical implants have not been eradicated. Most present coatings cannot simultaneously fulfil the requirements of antibacterial and antifungal activity as well as biocompatibility and reusability. Here, we report an antimicrobial hydrogel based on dimethyldecylammonium chitosan (with high quaternization)-graft-poly(ethylene glycol) methacrylate (DMDC-Q-g-EM) and poly(ethylene glycol) diacrylate, which has excellent antimicrobial efficacy against Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus and Fusarium solani. The proposed mechanism of the antimicrobial activity of the polycationic hydrogel is by attraction of sections of anionic microbial membrane into the internal nanopores of the hydrogel, like an 'anion sponge', leading to microbial membrane disruption and then microbe death. We have also demonstrated a thin uniform adherent coating of the hydrogel by simple ultraviolet immobilization. An animal study shows that DMDC-Q-g-EM hydrogel coating is biocompatible with rabbit conjunctiva and has no toxicity to the epithelial cells or the underlying stroma.
authors.affiliation: School of Chemical and Biomedical Engineering, Nanyang Technological University, 62 Nanyang Drive, Singapore 637459, Singapore.
authors.forename: Peng
authors.initials: P
authors.lastname: Li
authors.forename: Yin Fun
authors.initials: YF
authors.lastname: Poon
authors.forename: Weifeng
authors.initials: W
authors.lastname: Li
authors.forename: Hong-Yuan
authors.initials: HY
authors.lastname: Zhu
authors.forename: Siew Hooi
authors.initials: SH
authors.lastname: Yeap
authors.forename: Ye
authors.initials: Y
authors.lastname: Cao
authors.forename: Xiaobao
authors.initials: X
authors.lastname: Qi
authors.forename: Chuncai
authors.initials: C
authors.lastname: Zhou
authors.forename: Mouad
authors.initials: M
authors.lastname: Lamrani
authors.forename: Roger W
authors.initials: RW
authors.lastname: Beuerman
authors.forename: En-Tang
authors.initials: ET
authors.lastname: Kang
authors.forename: Yuguang
authors.initials: Y
authors.lastname: Mu
authors.forename: Chang Ming
authors.initials: CM
authors.lastname: Li
authors.forename: Matthew W
authors.initials: MW
authors.lastname: Chang
authors.forename: Susanna Su Jan
authors.initials: SS
authors.lastname: Leong
authors.forename: Mary B
authors.initials: MB
authors.lastname: Chan-Park
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology; D001672:Biocompatible Materials / Q000737:chemistry*; D048271:Chitosan / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects; D020100:Hydrogels / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D011073:Polyamines / Q000737:chemistry; D000071228:Polyelectrolytes; D011092:Polyethylene Glycols / Q000737:chemistry; D011109:Polymethacrylic Acids / Q000737:chemistry* / Q000494:pharmacology; D011817:Rabbits; D013211:Staphylococcus aureus / Q000187:drug effects; D013242:Sterilization; D013499:Surface Properties
chemical_list: D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D048271:Chitosan; D020100:Hydrogels; D011073:Polyamines; D000071228:Polyelectrolytes; D011092:Polyethylene Glycols; D011109:Polymethacrylic Acids; C556558:dimethyldecylammonium chitosan-graft-poly(ethylene glycol) methacrylate