title: [Effects of Rb1 and Rg1 on the expression of Bcl-2, Bax in apoptosis of HK-2 cells induced by the serum of kidney ischemia/reperfusion].
abstract: OBJECTIVE
  To explore effects of Ginsenosides (Rb1, Rg1) on the expression of Bcl-2, Bax in the serum of kidney ischemia/reperfusion inducing apoptosis of HK-2 cells.
  
  
  METHODS
  The serum of rabbits with renal ischemia/reperfusion (SIR) and the control serum of rabbits (SC) were acquired and cultured with HK-2 cells. Detected apoptosis with TUNEL assay. The experiment was designed as: control group,ischemia/ reperfusion group, Rb1 blocking group and Rg1 blocking group. To detect the expression of Bcl-2, Bax with immunocytochemistry after 24 hours' cultured.
  
  
  RESULTS
  The expression of Bax in Rb1 blocking group and Rg1 blocking group were significantly decreased (P < 0.01), the ratios of Bcl-2/Bax were increased as compared with ischemia/reperfusion group.
  
  
  CONCLUSIONS
  Rb1 and Rg1 have protective effects on apoptosis of HK-2 cells induced by serum of kidney ischemia/reperfusion.
authors.affiliation: Medical Department of Tibet National College, Xianyang 712082, China.
authors.forename: Min-Xia
authors.initials: MX
authors.lastname: Zhu
authors.forename: Bing
authors.initials: B
authors.lastname: Ran
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Feng
authors.forename: Qiang-Wen
authors.initials: QW
authors.lastname: Pan
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002460:Cell Line; D005260:Female; D036145:Ginsenosides / Q000494:pharmacology*; D007511:Ischemia / Q000097:blood; D007668:Kidney / Q000098:blood supply; D007687:Kidney Tubules, Proximal / Q000166:cytology; D008297:Male; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism*; D011817:Rabbits; D015427:Reperfusion Injury / Q000097:blood*; D044967:Serum / Q000502:physiology; D051028:bcl-2-Associated X Protein / Q000235:genetics / Q000378:metabolism*
chemical_list: C494828:Bax protein, rat; D036145:Ginsenosides; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; C442759:ginsenoside Rb1; C035054:ginsenoside Rg1