title: Rapid UPLC-MS/MS method for the determination of sufentanil in human plasma and its application in target-controlled infusion system.
abstract: A rapid, selective and highly sensitive ultra-performance liquid-chromatography mass-spectrometry (UPLC-MS/MS) method has been developed and validated for the determination of sufentanil in human plasma. Sufentanil was separated on an ACQUITY™ UPLC BEH C(18) column (50 mm × 2.1 mm, ID 1.7 μm) and analyzed in positive-ion (PI) electrospray-ionization (ESI) mode. The mobile phase (MP) consisted of acetonitrile:water (45:55, v/v) under isocratic conditions at a flow rate of 0.2 ml/min. Sufentanil and internal-standard (IS) fentanyl were eluted at 1.47 and 1.16 min, respectively, and their responses were optimized at the transitions m/z 387.2>238.0 and m/z 337.2>188.0, respectively. The calibration curve was linear over the range 0.071-4.56 ng/ml, with coefficients of determination >0.999. The accuracy and precision of the method were between 96.49% and 100.37% (RSD<9%), and the mean recovery of sufentanil was 84.08 ± 7.29%. The method was successfully applied to evaluate the predictive accuracy of Gepts pharmacokinetic sets in a target-controlled infusion (TCI) model, and the Gepts parameters were capable of predicting sufentanil plasma concentrations when multi-level target concentrations were acquired during surgery.
authors.affiliation: Department of Anesthesiology, Nanfang Hospital, Southern Medical University, 1838 Guangzhou Avenue North, Guangdong, Guangzhou, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Liang
authors.forename: Shanhe
authors.initials: S
authors.lastname: Wan
authors.forename: Jinfang
authors.initials: J
authors.lastname: Xiao
authors.forename: Jiajie
authors.initials: J
authors.lastname: Zhang
authors.forename: Miaoning
authors.initials: M
authors.lastname: Gu
mesh_terms: D000005:Abdomen / Q000601:surgery; D000328:Adult; D000701:Analgesics, Opioid / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D004355:Drug Stability; D004360:Drug Therapy, Computer-Assisted; D006801:Humans; D007260:Infusion Pumps; D008832:Microchemistry / Q000379:methods; D008875:Middle Aged; D008954:Models, Biological; D059035:Perioperative Period; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction; D021241:Spectrometry, Mass, Electrospray Ionization; D017409:Sufentanil / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D053719:Tandem Mass Spectrometry; D013678:Technology, Pharmaceutical; D055815:Young Adult
chemical_list: D000701:Analgesics, Opioid; D017409:Sufentanil