title: Spectroscopic optical coherence elastography.
abstract: We present an optical technique to image the frequency-dependent complex mechanical response of a viscoelastic sample. Three-dimensional hyperspectral data, comprising two-dimensional B-mode images and a third dimension corresponding to vibration frequency, were acquired from samples undergoing external mechanical excitation in the audio-frequency range. We describe the optical coherence tomography (OCT) signal when vibration is applied to a sample and detail the processing and acquisition techniques used to extract the local complex mechanical response from three-dimensional data that, due to a wide range of vibration frequencies, possess a wide range of sample velocities. We demonstrate frequency-dependent contrast of the displacement amplitude and phase of a silicone phantom containing inclusions of higher stiffness. Measurements of an ex vivo tumor margin demonstrate distinct spectra between adipose and tumor regions, and images of displacement amplitude and phase demonstrated spatially-resolved contrast. Contrast was also observed in displacement amplitude and phase images of a rat muscle sample. These results represent the first demonstration of mechanical spectroscopy based on B-mode OCT imaging. Spectroscopic optical coherence elastography (S-OCE) provides a high-resolution imaging capability for the detection of tissue pathologies that are characterized by a frequency-dependent viscoelastic response.
authors.affiliation: Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign, 405 N Mathews Avenue, Urbana, IL 61801, USA.
authors.forename: Steven G
authors.initials: SG
authors.lastname: Adie
authors.forename: Xing
authors.initials: X
authors.lastname: Liang
authors.forename: Brendan F
authors.initials: BF
authors.lastname: Kennedy
authors.forename: Renu
authors.initials: R
authors.lastname: John
authors.forename: David D
authors.initials: DD
authors.lastname: Sampson
authors.forename: Stephen A
authors.initials: SA
authors.lastname: Boppart
mesh_terms: D000818:Animals; D001943:Breast Neoplasms / Q000175:diagnosis* / Q000503:physiopathology*; D045744:Cell Line, Tumor; D055119:Elastic Modulus; D054459:Elasticity Imaging Techniques / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007089:Image Enhancement / Q000295:instrumentation / Q000379:methods; D007090:Image Interpretation, Computer-Assisted / Q000295:instrumentation* / Q000379:methods*; D019047:Phantoms, Imaging; D051381:Rats; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013057:Spectrum Analysis / Q000295:instrumentation*; D041623:Tomography, Optical Coherence / Q000295:instrumentation*