title: [Identification biomarkers for cervical cancer in peripheral blood lymphocytes by oligonucleotide microarrays].
abstract: OBJECTIVE
  To identify the molecular biomarkers for cervical cancer in peripheral blood lymphocytes by oligonucleotide microarrays.
  
  
  METHODS
  Human genome oligonucleotide microarray analysis included 4 early-stage cervical cancer patients and 3 controls. The selected genes from the microarray analysis were validated in additional 20 early-stage cervical cancer patients and 15 controls by real-time reverse-transcription polymerase chain reaction (RT-PCR).
  
  
  RESULTS
  Genes identified by gene selection program were expressed differently in the blood samples of early-stage cervical cancer from those of healthy controls. To validate the gene expression data, 5 genes were analyzed by real-time RT-PCR. In three of 5 identified genes, tenascin-c, nucleolin, and enolase 2 (ENO2) showed a significant up-regulation in blood samples of early-stage cervical cancer patients versus that of the controls.
  
  
  CONCLUSIONS
  The up-regulation of tenascin-c, nucleolin and ENO2 in peripheral blood may be used to identify novel blood biomarkers for detecting cervical cancer in a clinically accessible surrogate tissue. Thus it may offer a possibility of developing a non-invasive and predictive diagnostic tool for the disease.
authors.affiliation: Department of Gynecology, Beijing Obstetrics & Gynecology Hospital, Capital Medical University, Beijing 100026, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Sheng
authors.forename: Wei-yuan
authors.initials: WY
authors.lastname: Zhang
mesh_terms: D000328:Adult; D014408:Biomarkers, Tumor / Q000097:blood*; D016022:Case-Control Studies; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D002583:Uterine Cervical Neoplasms / Q000097:blood* / Q000235:genetics
chemical_list: D014408:Biomarkers, Tumor