title: [Effects of hypercapnia on nuclear factor-kappaB and TNF-alpha in acute lung injury models].
abstract: OBJECTIVE
  To observe the effects of hypercapnia on nuclear factor-kappaB and TNF-alpha in acute lung injury models.
  
  
  METHODS
  Six of the twenty-two healthy New Zealand white rabbits were randomly allocated to control group (Group C), the other sixteen rabbits were injected with oleic acid (0.1 ml/kg) intravenously, then were randomized to normocapnic group (Group N, n = 8) versus hypercapnic group (Group H, n = 8). TNF-alpha of serum and bronchoalveolar lavage fluid (BALF) and the expression of nuclear factor-kappaB in the lung were analyzed after three hours' mechanical ventilation.
  
  
  RESULTS
  TNF-alpha of serum and bronchoalveolar lavage fluid in Group N and H was significantly higher than that in Group C (P < 0.01), and that of Group N was higher than that of Group H (P < 0.05). The expression of nuclear factor-kappaB in Group H was less than that in Group N by both immunohistochemistry and Western-blot analysis. Peak airway pressure in Group H was significantly lower than that in Group N and the dynamic lung compliance was significantly higher than that in Group N (P < 0.05). PaO2 in Group H was significantly higher than that in Group N (P < 0.05). Histologic damage in Group N was significantly severer than that in Group H.
  
  
  CONCLUSIONS
  Hypercapnia is protective in this in vivo model of ALl. The mechanisms might be associated with the prohibition of nuclear factor-kappaB and then the decreased expression of TNF-alpha by hypercapnia.
authors.affiliation: Emergency Department of the First Hospital Affiliated to China Medical University, Shenyang 110001, China.
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Yang
authors.forename: Xin-Ping
authors.initials: XP
authors.lastname: Ji
authors.forename: Zhi
authors.initials: Z
authors.lastname: Liu
authors.forename: Gang
authors.initials: G
authors.lastname: Liu
authors.forename: Fu-Lan
authors.initials: FL
authors.lastname: Guan
mesh_terms: D055371:Acute Lung Injury / Q000378:metabolism*; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D006935:Hypercapnia / Q000378:metabolism*; D016328:NF-kappa B / Q000097:blood / Q000378:metabolism*; D011817:Rabbits; D014409:Tumor Necrosis Factor-alpha / Q000097:blood / Q000378:metabolism*
chemical_list: D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha