title: [mRNA expression of activity-regulated cytoskeletal-associated protein in hippocampus induced by insular-kindled rats].
abstract: OBJECTIVE
  To investigate the expression of Arc mRNA in insular electrical kindled and single electrical stimulated rats and its significance.
  
  
  METHODS
  Male SD rats were divided randomly into kindled group, single electrical stimulated group, sham-operated group and control group. Each group was divided into 2 sub-groups at different time points. Kindled group: establishing chronic insular electrical kindled model, decapitation to perform RT-PCR of Arc mRNA on hippocampus and to applying in situ hybridization of Arc mRNA on dentate gyrus; Single electrical stimulated group: using the same method as the kindled group with only electrical stimulation twice; Sham-operated group: using the same method to the kindled group without electrical stimulation;
  
  
  METHODS
  no surgery.
  
  
  RESULTS
  Expression of Arc mRNA in the hippocampus of single electrical stimulated, sham-operated and control groups were 0.72 ± 0.14, 0.75 ± 0.16 and 0.71 ± 0.14 respectively. They were significantly less than the kindled group of 1.78 ± 0.43(P < 0.01) at 3 h. The expression of Arc mRNA had no significant difference (P > 0.05) among 4 groups at 6 h; in situ hybridization (cell count) of Arc mRNA showed the expression of Arc mRNA in the kindled group was 112.8 ± 6.0. And it was significantly higher than the other three groups of 46.25 ± 4.35, 45.25 ± 6.23, 44.75 ± 6.49 (P < 0.01) after 3 h, there were no significant difference among 4 groups at 6 h (P > 0.05).
  
  
  CONCLUSIONS
  Insular epilepsy increase the expression of Arc mRNA in hippocampus. Arc may play an important role in the synaptic plasticity of insular epilepsy.
authors.affiliation: Department of Neurosurgery, Affiliated Hospital of Ningxia Medical University, Key Lab of Craniocerbral Diseases, Ningxia Hui Autonomous Region, Yinchuan 750004, China.
authors.forename: Pei-song
authors.initials: PS
authors.lastname: Zhang
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.forename: Qing-zhu
authors.initials: QZ
authors.lastname: Liu
authors.forename: Wen-zhong
authors.initials: WZ
authors.lastname: Xu
authors.forename: Jiang-hua
authors.initials: JH
authors.lastname: Qi
authors.forename: Zheng
authors.initials: Z
authors.lastname: Liu
authors.forename: Tao
authors.initials: T
authors.lastname: Sun
mesh_terms: D000818:Animals; D002540:Cerebral Cortex / Q000378:metabolism; D003598:Cytoskeletal Proteins / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D006624:Hippocampus / Q000378:metabolism*; D007696:Kindling, Neurologic / Q000235:genetics* / Q000378:metabolism; D008297:Male; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D003598:Cytoskeletal Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; C092069:activity regulated cytoskeletal-associated protein