title: [Level and effect of anti-HSP70 antibody changed in the procession of rat atherosclerosis].
abstract: OBJECTIVE
  To explore the level of anti-HSP70 antibody in plasma during atherosclerosis procedure induced by high-fat diet in rat and the relationship of them.
  
  
  METHODS
  Twenty eight rat were divided into high-fat diet group (H) and control group (C). The total cholesterol (TC), Glyceride (TG), low density lipoprotein cholesterol (LDL-C) in serum, pathology change of rat Arch of the aorta were determined, the level of anti-HSP70 antibody and their Phenotype were evaluated by ELISA.
  
  
  RESULTS
  After two weeks, the serum concentrations of TC and LDL-C in rat supplemented by high-fat diet were significantly higher than those in control group (P < 0.01), the serum TG were much lower than those in control group (P < 0.01). Four weeks later the level of anti-HSP70 antibody, IgM, IgG phenotype were significantly higher than those in control group (P < 0.01). There were lipin deposition and mottling formation in rat Arch of the aorta in rat supplemented by high-fat diet in 12th week.
  
  
  CONCLUSIONS
  Atherosclerosis could be induced by high-fat diet in rat. Accompany with the atherosclerosis procession, the level of anti-HSP70 antibody was continuously elevated, the level of anti-HSP70 antibody was related to atherosclerosis. The level of anti-HSP70 antibody was closely associated with atherosclerosis.
authors.affiliation: Institute of Health and Environmental Medicine, Academy of Military Medical Sciences, Tianjin 300050, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Leng
authors.forename: Rui
authors.initials: R
authors.lastname: Zhan
authors.forename: Xin-Xing
authors.initials: XX
authors.lastname: Wang
authors.forename: Xiao-Hua
authors.initials: XH
authors.lastname: Lu
authors.forename: Li-Qun
authors.initials: LQ
authors.lastname: Wang
authors.forename: Xiu-Jie
authors.initials: XJ
authors.lastname: Gao
authors.forename: Ling-Jia
authors.initials: LJ
authors.lastname: Qian
mesh_terms: D000818:Animals; D000906:Antibodies / Q000097:blood*; D050197:Atherosclerosis / Q000276:immunology* / Q000503:physiopathology*; D059305:Diet, High-Fat / Q000009:adverse effects; D004041:Dietary Fats / Q000008:administration & dosage; D018840:HSP70 Heat-Shock Proteins / Q000276:immunology*; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D008297:Male; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000906:Antibodies; D004041:Dietary Fats; D018840:HSP70 Heat-Shock Proteins; D007074:Immunoglobulin G; D007075:Immunoglobulin M