title: [Role of extracellular-signal regulated kinase cascade on cerebral ischemia and ischemic preconditioning in hippocampal neuron in gerbils].
abstract: OBJECTIVE
  To investigate the role of extracellular-signal regulated kinase (ERK) cascade on cerebral ischemia and ischemic preconditioning in hippocampal neuron.
  
  
  METHODS
  Male gerbils were randomly divided into sham group (SH), ischemia/reperfusion group (I/ R), ischemia preconditioning group (IP), specific antagonist of ERK-PD98059 (PD), solvent control groups (VE group), PD98059 combined with IP group (PIP). Forebrain ischemia was induced by occlusion of bilateral common carotid arteries and confirmed by isoelectricity of EEG. Observations were carried out in each group 15 min, 2 h, 4 h, 6 h, 1 d, 3 d, 5 d and 7 d after ischemia. Open field test was used to examine the spontaneous motor activity, the survival and apoptotic neurons, Fos and NF-kappaB masculine neurons in hippocampal CA1 region were counted, the expression of HSP70 in hippocampal CA1 region and p-ERK in hippocampal CA3/DG regions were detected by SABC immunocytochemical technique.
  
  
  RESULTS
  The spontaneous motor activity, the number of apoptotic neurons and NF-kappaB masculine neurons at 1 d, 3 d, 5 d, 7 d in CA1 region were much less in IP group than in I/R group (P < 0.01). The number of Fos masculine neurons at 15 min, 2 h, 4 h, 6 h, 1 d in CA1 region were significant more in IP group than in I/R group (P < 0.01). The expressions of p-ERK and HSP70 were significantly higher in IP group than in I/R group. The number of Fos masculine neurons at each point were more and apoptotic neurons at 1 d, 3 d were less in PD group than in I/R group. Results of observation in PIP group were within IP group and I/R group.
  
  
  CONCLUSIONS
  Activation of ERK in CA3/DG regions were related to ischemic tolerance. Induction of the expression of Fos and HSP70, decreasing of the product of NF-kB which might be one of the molecule mechanisms playing an important role in neural protection of ischemic preconditioning.
authors.affiliation: Department of Anesthesiology, The 2nd affiliated hospital of Wenzhou Medical College, Wenzhou 325027, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.forename: Hong
authors.initials: H
authors.lastname: Cao
authors.forename: Qing-Quan
authors.initials: QQ
authors.lastname: Lian
authors.forename: Yao-Qi
authors.initials: YQ
authors.lastname: Wang
authors.forename: Yin-Ming
authors.initials: YM
authors.lastname: Zeng
authors.forename: Shang-Long
authors.initials: SL
authors.lastname: Yao
authors.forename: Bang-Xiong
authors.initials: BX
authors.lastname: Zeng
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000378:metabolism* / Q000517:prevention & control; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism*; D005849:Gerbillinae; D006624:Hippocampus / Q000098:blood supply / Q000166:cytology; D019194:Ischemic Preconditioning; D008297:Male; D016328:NF-kappa B / Q000378:metabolism; D009474:Neurons / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology
chemical_list: D016328:NF-kappa B; D048049:Extracellular Signal-Regulated MAP Kinases