title: Expression and localization of messenger RNA for tumor necrosis factor receptor (TNF-R) I and TNF-RII in pregnant mouse uterus and placenta.
abstract: The temporal and cell-specific localization of tumor necrosis factor (TNF) receptor mRNAs in the uterus and placenta during pregnancy in the mouse was investigated. Messenger RNA for TNF and the TNF receptors (TNF-RI, p55/p60 and TNF-RII, p75/p80) was assessed by northern blot andin situ hybridization. TNF, TNF-RI and TNF-RII specific transcripts were present on days 7 through 18 of pregnancy. Relative concentrations of TNF mRNA decreased from days 7 to 18 with levels being higher in the uterus than the placenta. In contrast TNF-RI mRNA levels were constant throughout gestation and no differences were seen between steady state levels in the uterus and placenta. Two transcripts for TNF-RII (3.6 and 4.5 kb) were identified in all tissues. Steady state levels of TNF-RII mRNA increased throughout gestation and levels were higher in the placenta than in the uterus. On day 9 of gestation, TNF-RI and TNF-RII mRNAs were localized to undecidualized endometrium, mesometrial decidual cells, and the developing placenta. In addition, muscle cells contained TNF-RI but not TNF-RII mRNA. By day 15 of gestation, TNF-RI and TNF-RII transcripts were primarily localized to the uterine epithelium and trophoblast giant cells and spongiotrophoblast cells in the placenta. The results of these studies reveal the uterine and placental cell-specific expression of TNF receptor mRNAs during pregnancy in the mouse and provide insight into the cellular targets of TNF action.
authors.affiliation: Department of Anatomy and Cell Biology, University of Kansas Medical Center, 66160-7400, Kansas City, Kansas, USA.
authors.forename: K F
authors.initials: KF
authors.lastname: Roby
authors.forename: N
authors.initials: N
authors.lastname: Laham
authors.forename: H
authors.initials: H
authors.lastname: Kröning
authors.forename: P F
authors.initials: PF
authors.lastname: Terranova
authors.forename: J S
authors.initials: JS
authors.lastname: Hunt