title: Study on the ternary mixed ligand complex of palladium(II)-aminophylline-fluorescein sodium by resonance Rayleigh scattering, second-order scattering and frequency doubling scattering spectrum and its analytical application.
abstract: The interaction between palladium(II)-aminophylline and fluorescein sodium was investigated by resonance Rayleigh scattering, second-order scattering and frequency doubling scattering spectrum. In pH 4.4 Britton-Robinson (BR) buffer medium, aminophylline (Ami) reacted with palladium(II) to form chelate cation([Pd(Ami)]2+), which further reacted with fluorescein sodium (FS) to form ternary mixed ligand complex [Pd(Ami)(FS)2]. As a result, resonance Rayleigh scattering (RRS), second-order scattering (SOS) and frequency doubling scattering spectrum (FDS) were enhanced. The maximum scattering wavelengths of [Pd(Ami)(FS)2] were located at 300 nm (RRS), 650 nm (SOS) and 304 nm (FDS). The scattering intensities were proportional to the Ami concentration in a certain range and the detection limits were 7.3 ng mL(-1) (RRS), 32.9 ng mL(-1) (SOS) and 79.1 ng mL(-1) (FDS), respectively. Based on it, the new simple, rapid, and sensitive scattering methods have been proposed to determine Ami in urine and serum samples. Moreover, the formation mechanism of [Pd(Ami)(FS)2] and the reasons for enhancement of RRS were fully discussed.
authors.affiliation: Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.
authors.forename: Peili
authors.initials: P
authors.lastname: Chen
authors.forename: Shaopu
authors.initials: S
authors.lastname: Liu
authors.forename: Zhongfang
authors.initials: Z
authors.lastname: Liu
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Hu
mesh_terms: D000077410:Aluminum Chloride; D017607:Aluminum Compounds; D000628:Aminophylline / Q000737:chemistry*; D000818:Animals; D002417:Cattle; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002712:Chlorides; D002942:Circular Dichroism; D003300:Copper; D019793:Fluorescein / Q000737:chemistry*; D008024:Ligands; D008027:Light; D010165:Palladium / Q000737:chemistry*; D017433:Protein Structure, Secondary; D012542:Scattering, Radiation; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism; D012965:Sodium Chloride; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
chemical_list: D017607:Aluminum Compounds; D002712:Chlorides; D008024:Ligands; D012710:Serum Albumin, Bovine; D000628:Aminophylline; D000077410:Aluminum Chloride; D012965:Sodium Chloride; D010165:Palladium; D003300:Copper; D019793:Fluorescein