title: Detection and quantitation of soluble B7-H3 in expressed prostatic secretions: a novel marker in patients with chronic prostatitis.
abstract: OBJECTIVE
  We determined the soluble B7-H3 level and its clinical significance in serum and expressed prostatic secretions of patients with chronic prostatitis, including chronic bacterial prostatitis (type II) and chronic pelvic pain syndrome.
  
  
  METHODS
  Using enzyme-linked immunosorbent assay we measured soluble B7-H3 in 11 patients with chronic prostatitis (type II), and 26 with inflammatory (type IIIA) and 54 with noninflammatory (type IIIB) chronic pelvic pain syndrome, and healthy donors. We assessed differences between these groups using Student's t test. As determined by the National Institutes of Health-Chronic Prostatitis Symptom Index, we correlated soluble B7-H3 with clinical pain using the Pearson test.
  
  
  RESULTS
  We found no significant difference between serum soluble B7-H3 in healthy donors and patients with chronic prostatitis (p = 0.897). However, soluble B7-H3 in expressed prostatic secretions was statistically significantly decreased in patients with chronic prostatitis vs controls (p <0.001). ROC using soluble B7-H3 greater than 38.82 ng/ml in expressed prostatic secretions distinguished patients with chronic prostatitis from healthy donors with 90.9% sensitivity and 83.5% specificity. Also, soluble B7-H3 levels in expressed prostatic secretions correlated negatively with the Chronic Prostatitis Symptom Index and the pain subscore. Compared to the pretreatment level soluble B7-H3 in expressed prostatic secretions was significantly increased in patients with a greater than 25% decrease in the Chronic Prostatitis Symptom Index total score (p = 0.016).
  
  
  CONCLUSIONS
  Data indicate that the soluble B7-H3 level in expressed prostatic secretions is a novel chronic prostatitis marker that correlates negatively with subjective symptoms.
authors.affiliation: Department of Urology, First Affiliated Hospital of Soochow University, Suzhou, People's Republic of China.
authors.forename: Xuedong
authors.initials: X
authors.lastname: Wei
authors.forename: Guangbo
authors.initials: G
authors.lastname: Zhang
authors.forename: Hexing
authors.initials: H
authors.lastname: Yuan
authors.forename: Xiang
authors.initials: X
authors.lastname: Ding
authors.forename: Shuxiang
authors.initials: S
authors.lastname: Li
authors.forename: Xueguang
authors.initials: X
authors.lastname: Zhang
authors.forename: Jianquan
authors.initials: J
authors.lastname: Hou
mesh_terms: D000328:Adult; D000367:Age Factors; D015703:Antigens, CD / Q000032:analysis* / Q000378:metabolism; D060887:B7 Antigens; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D012634:Bodily Secretions / Q000737:chemistry*; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008297:Male; D008875:Middle Aged; D010147:Pain Measurement; D017699:Pelvic Pain / Q000097:blood / Q000175:diagnosis / Q000188:drug therapy / Q000209:etiology; D011379:Prognosis; D011467:Prostate / Q000378:metabolism*; D011472:Prostatitis / Q000097:blood / Q000150:complications / Q000175:diagnosis / Q000188:drug therapy / Q000378:metabolism*; D012372:ROC Curve; D011971:Receptors, Immunologic / Q000032:analysis* / Q000378:metabolism; D012016:Reference Values; D018570:Risk Assessment; D012680:Sensitivity and Specificity; D012720:Severity of Illness Index; D012737:Sex Factors; D012995:Solubility; D055815:Young Adult
chemical_list: D015703:Antigens, CD; D060887:B7 Antigens; D015415:Biomarkers; C423237:CD276 protein, human; D011971:Receptors, Immunologic