title: Determination of non-protein cysteine in human serum by a designed BODIPY-based fluorescent probe.
abstract: A novel fluorescent probe is designed and synthesized for the determination of cysteine in biological samples by incorporating 2,4-dinitrobenzenesulfonyl (DBS) group as a quencher into the BODIPY skeleton. The BODIPY-based probe itself shows weak fluorescence due to the strong intramolecular charge transfer process. Upon reaction with cysteine, however, the probe produces a rapid and large fluorescence enhancement through the removal of the DBS unit by nucleophilic aromatic substitution. This valuable property leads to the development of a new and simple method for cysteine assay. Under the optimized conditions, the fluorescence enhancement value is directly proportional to the concentration of cysteine in the range 2-12 μM, with a detection limit of 30 nM (S/N=3). The applicability of the developed method has been successfully demonstrated on the determination of non-protein cysteine in human serum. Compared to most of the existing fluorescent probes proposed for cysteine, the BODIPY-based one exhibits an excellent overall performance in terms of selectivity, sensitivity and simplicity.
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, No. 2, Beiyijie, Zhongguancun, Haidian District, Beijing 100190, China.
authors.forename: Jinxin
authors.initials: J
authors.lastname: Lu
authors.forename: Chengdong
authors.initials: C
authors.lastname: Sun
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.forename: Huimin
authors.initials: H
authors.lastname: Ma
authors.forename: Wen
authors.initials: W
authors.lastname: Shi
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Li
mesh_terms: D001557:Benzenesulfonates / Q000737:chemistry; D001774:Blood Chemical Analysis / Q000379:methods*; D001896:Boron Compounds / Q000737:chemistry*; D003545:Cysteine / Q000097:blood* / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D016014:Linear Models; D013050:Spectrometry, Fluorescence; D013696:Temperature; D013997:Time Factors
chemical_list: C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001557:Benzenesulfonates; D001896:Boron Compounds; D005456:Fluorescent Dyes; C001073:2,4-dinitrobenzenesulfonic acid; D003545:Cysteine