title: Bioadhesion of various proteins on random, diblock and triblock copolymer surfaces and the effect of pH conditions.
abstract: The adhesive interactions of block copolymers composed of poly(methyl methacrylate) (PMMA)/poly(acrylic acid) (PAA) and poly(methyl methacrylate)/poly(2-hydroxyethyl methacrylate) (PHEMA) with the proteins fibronectin, bovine serum albumin and collagen were studied by atomic force microscopy. Adhesion experiments were performed both at physiological pH and at a slightly more acidic condition (pH 6.2) to model polymer-protein interactions under inflammatory or infectious conditions. The PMMA/PAA block copolymers were found to be more sensitive to the buffer environment than PMMA/PHEMA owing to electrostatic interactions between the ionized acrylate groups and the proteins. It was found that random, diblock and triblock copolymers exhibit distinct adhesion profiles although their chemical compositions are identical. This implies that biomaterial nanomorphology can be used to control protein-polymer interactions and potentially cell adhesion.
authors.affiliation: Nanoprobe Laboratory for Bio- and Nanotechnology and Biomimetics, The Ohio State University, Columbus, OH 43210, USA.
authors.forename: Manuel L B
authors.initials: ML
authors.lastname: Palacio
authors.forename: Scott R
authors.initials: SR
authors.lastname: Schricker
authors.forename: Bharat
authors.initials: B
authors.lastname: Bhushan
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D000268:Adhesiveness; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002417:Cattle; D002448:Cell Adhesion; D003094:Collagen / Q000737:chemistry; D005353:Fibronectins / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D018625:Microscopy, Atomic Force / Q000379:methods; D008954:Models, Biological; D008956:Models, Chemical; D036103:Nanotechnology / Q000379:methods; D011102:Polyhydroxyethyl Methacrylate / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D011109:Polymethacrylic Acids / Q000737:chemistry; D011506:Proteins / Q000737:chemistry; D012709:Serum Albumin / Q000737:chemistry; D013499:Surface Properties; D017155:Wettability
chemical_list: D000180:Acrylic Resins; D001672:Biocompatible Materials; D005353:Fibronectins; D011108:Polymers; D011109:Polymethacrylic Acids; D011506:Proteins; D012709:Serum Albumin; C030613:polymethacrylic acid; D011102:Polyhydroxyethyl Methacrylate; C006903:carbopol 940; D003094:Collagen