title: [The transfection and expression of retro viral-mediated human FL cDNA in bone marrow stromal cell line].
abstract: OBJECTIVE
  The expression of retroviral-mediated FL gene transfer into bone marrow stromal cell line HFCL was studied.
  
  
  METHODS
  FLT3 ligand (FL) cDNA was recombined with retroviral vector pLXSN by gene recombination technology. The recombinant plasmid was transferred into retrovirus packaging cell line PA3 17 by lipofectamine, and the resistant clones were selected by G418 selective medium. The mRNA expression in HFCL cells and integration of genome DNA were assayed by RT-PCR and genomic DNA PCR. The biological activity of FL in the culture was investigated by mouse bone marrow CFU-GM assay.
  
  
  RESULTS
  The recombinant plasmid pLFSN was successfully constructed. The expression of FL mRNA was detected in HFCL cells. In the genome of these infected target cells, neo gene and FL cDNA were successfully expressed. The biological activity of FL in the culture demonstrated that HFCL cells transfected with FL could significantly augment FL in vitro.
  
  
  CONCLUSIONS
  These results suggest that bone marrow stromal cell lines might become target cells of gene therapy.
authors.affiliation: Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850.
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
authors.forename: X S
authors.initials: XS
authors.lastname: Li
authors.forename: F Z
authors.initials: FZ
authors.lastname: Jiang
authors.forename: N
authors.initials: N
authors.lastname: Mao
authors.forename: P H
authors.initials: PH
authors.lastname: Tang
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D002460:Cell Line; D015870:Gene Expression; D005822:Genetic Vectors; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics*; D051379:Mice; D010957:Plasmids; D012190:Retroviridae / Q000235:genetics; D014162:Transfection
chemical_list: D008565:Membrane Proteins; C084881:flt3 ligand protein