title: Megadose vitamin C suppresses sulfoconjugation in human colon carcinoma cell line Caco-2.
abstract: Intake of high doses of vitamin C has known to modulate sulfoconjugation of drugs in the intestine, but the underlying mechanisms for this effect remain to be elucidated. In the present study, we investigated the effects of vitamin C (l-ascorbic acid (AA)) on sulfation of 1-naphthol using Caco-2 cells, a model of human intestinal cells. We found that high dose of AA inhibited the accumulation of 1-naphthyl sulfate in Caco-2 culture medium within 24h in a dose-dependent manner (IC(50)=42 mM). Dehydroascorbic acid (DA), an oxidized form of AA, showed no inhibition. AA did not inhibit the in vitro sulfotransferase (SULT) activity toward 1-naphthol, whereas it reduced the expression of genes belonging to SULT1A family, SULT1A1 and SULT1A3. DA showed no effect on SULT1A gene expression. Consistent with the reduction in gene expression, AA reduced the cytosolic SULT activity towards 1-naphthol in the AA-treated Caco-2 cells. In addition, cAMP exerted an additive effect on AA-mediated repression of SULT1A gene expression. Our results suggest that megadose AA suppresses sulfoconjugation in the intestine mainly by downregulating the expression of SULT1A genes.
authors.affiliation: Keio University, Faculty of Pharmacy, Shibakoen, Minatoku, Tokyo, Japan.
authors.forename: Hidetaka
authors.initials: H
authors.lastname: Tsuruta
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Yagishita
authors.forename: Mikiko
authors.initials: M
authors.lastname: Shimizu
authors.forename: Hiroomi
authors.initials: H
authors.lastname: Tamura
mesh_terms: D015239:Arylsulfotransferase / Q000037:antagonists & inhibitors / Q000235:genetics; D001205:Ascorbic Acid / Q000494:pharmacology*; D018938:Caco-2 Cells; D000242:Cyclic AMP / Q000494:pharmacology; D006801:Humans; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism*; D009284:Naphthols / Q000378:metabolism*; D013463:Sulfuric Acid Esters / Q000378:metabolism*
chemical_list: D009284:Naphthols; D013463:Sulfuric Acid Esters; C029092:naphthyl sulfate; C029350:1-naphthol; D000242:Cyclic AMP; D015239:Arylsulfotransferase; C117544:SULT1A1 protein, human; D001205:Ascorbic Acid