title: SYBR green real-time PCR used to detect celery in food.
abstract: Celery was found to provoke human allergenic response in some countries. Labeling of celery ingredients was required by the European Union, and the threshold set at 10 mg/kg (0.001%). In our study, a celery mannitol transporter (Mat3) gene-based detection method was established by means of SYBR Green real-time PCR technique. No cross-reactivity was found between celery and the other food materials. Absolute detection limit (LODa), relative detection limit (LODr), and practical detection limit (LODp) of the method were determined through experiments on pure celery DNA, DNA mix, and spiked food samples. The method was able to detect 0.001% raw food sample and 0.01% heated food sample. The utility of the method was confirmed by the investigation of 13 commercial foods.
authors.affiliation: Chinese Academy of Inspection and Quarantine, No. 3, Gaobeidian North St, Chaoyang District, Beijing, 100123, People's Republic of China.
authors.forename: Yajun
authors.initials: Y
authors.lastname: Wu
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.forename: Yunhua
authors.initials: Y
authors.lastname: Gao
authors.forename: Liqun
authors.initials: L
authors.lastname: Bai
authors.forename: Haiyan
authors.initials: H
authors.lastname: Wang
mesh_terms: D000077102:Apium / Q000235:genetics*; D001483:Base Sequence; D052160:Benzothiazoles; D018744:DNA, Plant / Q000032:analysis*; D003959:Diamines; D005504:Food Analysis / Q000379:methods*; D057230:Limit of Detection; D008969:Molecular Sequence Data; D009930:Organic Chemicals; D016133:Polymerase Chain Reaction / Q000379:methods*; D011804:Quinolines
chemical_list: D052160:Benzothiazoles; D018744:DNA, Plant; D003959:Diamines; D009930:Organic Chemicals; D011804:Quinolines; C098022:SYBR Green I