title: Photoactivable peptides for identifying enzyme-substrate and protein-protein interactions.
abstract: Photoactivated cross-linking of peptides to proteins is a useful strategy for identifying enzyme-substrate and protein-protein interactions in cell lysates as demonstrated by studies on the human hypoxia inducible factor system.
authors.affiliation: University of Oxford, Department of Chemistry and the Oxford Centre for Integrative Systems Biology, Chemistry Research Laboratory, Mansfield Road, Oxford, OX1 3TA, United Kingdom.
authors.forename: Dante
authors.initials: D
authors.lastname: Rotili
authors.forename: Mikael
authors.initials: M
authors.lastname: Altun
authors.forename: Refaat B
authors.initials: RB
authors.lastname: Hamed
authors.forename: Christoph
authors.initials: C
authors.lastname: Loenarz
authors.forename: Armin
authors.initials: A
authors.lastname: Thalhammer
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Hopkinson
authors.forename: Ya-Min
authors.initials: YM
authors.lastname: Tian
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Ratcliffe
authors.forename: Antonello
authors.initials: A
authors.lastname: Mai
authors.forename: Benedikt M
authors.initials: BM
authors.lastname: Kessler
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Schofield
mesh_terms: D002478:Cells, Cultured; D003432:Cross-Linking Reagents / Q000494:pharmacology*; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000737:chemistry / Q000378:metabolism*; D007668:Kidney / Q000166:cytology / Q000378:metabolism; D015335:Molecular Probes; D010446:Peptide Fragments / Q000737:chemistry / Q000528:radiation effects*; D010777:Photochemistry; D025941:Protein Interaction Mapping; D021241:Spectrometry, Mass, Electrospray Ionization; D051794:Von Hippel-Lindau Tumor Suppressor Protein / Q000737:chemistry / Q000378:metabolism*
chemical_list: D003432:Cross-Linking Reagents; C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D015335:Molecular Probes; D010446:Peptide Fragments; D051794:Von Hippel-Lindau Tumor Suppressor Protein; C497446:VHL protein, human