title: [Effect of injection salvia miltiorrhiza on the expression of inducible nitric oxide synthase in the cochlea of guinea pig damaged by streptomycin].
abstract: OBJECTIVE
  To investigate the expression of Inducible Nitric Oxide Synthase (iNOS) in the cochlea of guinea pig after streptomycin (SM) and the antagonism of Salvia Miltiorrhiza injection on SM ototoxicity.
  
  
  METHODS
  Light microscope, transmission electron microscope (TEM), immunohistochemical staining and image quantitative analysis technique, combined with auditory brainstem response (ABR) measurement were used.
  
  
  RESULTS
  After 10 days by drugs, the threshold of ABR of SM increased significantly, and the threshold of ABR of DS+ SM reduced more than that of SM (P < 0.01), the Corti's organ (CO), inner hair cell (IHC) and outer hair cell (OHC), spiral ganglion(SG), stria vascularis (SV) of SM damaged more greatly than that of DS + SM under light microscope and TEM. The result of immunohistochemical staining implied the expression of iNOS in CO, IHC and OHC, SG, SV of SM higher than that of DS+ SM.
  
  
  CONCLUSIONS
  The threshold of ABR increases and the expression of iNOS strengthens on SM ototoxicity. DS can reduce the increasing and inhibit the over-expression of iNOS, and reduce the damage of SM ototoxicity, which implies the protective role of DS on SM ototoxicity.
authors.affiliation: Hearing Research Laboratory, Basic Medical Department, China Medical University, Shenyang 110001, China.
authors.forename: Li-juan
authors.initials: LJ
authors.lastname: Shi
authors.forename: Hao
authors.initials: H
authors.lastname: Tang
mesh_terms: D000818:Animals; D003051:Cochlea / Q000187:drug effects* / Q000378:metabolism*; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000494:pharmacology; D006168:Guinea Pigs; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism*; D027561:Salvia miltiorrhiza; D013307:Streptomycin / Q000506:poisoning*
chemical_list: D004365:Drugs, Chinese Herbal; D052247:Nitric Oxide Synthase Type II; D013307:Streptomycin