title: Biphasic effect of nitric oxide on the cardiac voltage-dependent anion channel.
abstract: Nitric oxide (NO·) effects on the cardiac mitochondrial voltage-dependent anion channel (VDAC) are unknown. The effects of exogenous NO· on VDAC purified from rat hearts were investigated in this study. When incorporated into lipid bilayers, VDAC was inhibited directly by an NO· donor, PAPA NONOate, in a concentration-dependent biphasic manner. This was prevented by an NO· scavenger, 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide. The effect paralleled that of NO() in delaying the opening of the mitochondrial permeability transition (PT) pore. These biphasic effects on the cardiac VDAC and the mitochondrial PT pore reveal a tandem impact of NO() on the two mitochondrial entities.
authors.affiliation: Department of Anesthesiology, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
authors.forename: Qunli
authors.initials: Q
authors.lastname: Cheng
authors.forename: Filip
authors.initials: F
authors.lastname: Sedlic
authors.forename: Danijel
authors.initials: D
authors.lastname: Pravdic
authors.forename: Zeljko J
authors.initials: ZJ
authors.lastname: Bosnjak
authors.forename: Wai-Meng
authors.initials: WM
authors.lastname: Kwok
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D022062:Electrophysiologic Techniques, Cardiac; D006321:Heart / Q000502:physiology; D033681:Mitochondrial Membrane Transport Proteins / Q000187:drug effects; D000083162:Mitochondrial Permeability Transition Pore; D009206:Myocardium / Q000737:chemistry / Q000378:metabolism; D009569:Nitric Oxide / Q000494:pharmacology*; D051381:Rats; D050994:Voltage-Dependent Anion Channels / Q000187:drug effects*
chemical_list: D033681:Mitochondrial Membrane Transport Proteins; D000083162:Mitochondrial Permeability Transition Pore; D050994:Voltage-Dependent Anion Channels; D009569:Nitric Oxide