title: [Recycle of spent cells from anaerobic succinate fermentation].
abstract: Spent cells recovered from anaerobic fermentation by Actinobacillus succinogenes were used as nitrogen source for succinic acid production. Three methods were investigated for cell wall-breaking. The results showed that enzymatic hydrolysis was more effective for higher succinic acid yield. When the enzymatic hydrolysate of spent cells was added to reach a total nitrogen concentration 1.11 g/L (equivalent to 10 g/L yeast extract), the succinic acid concentration was 42.0 g/L, but it increased slightly when enhancing the level of enzymatic hydrolysate. However, when 5 g/L yeast extract was supplemented with the enzymatic hydrolysate of spent cells, the succinic acid concentration reached 75.5 g/L after 36 hours and, the succinic acid productivity was 2.10 g/(L x h), which increased by 66.7% compared with the fermentation using 10 g/L yeast extract. Therefore, enzymatic hydrolysate of spent cells could replace 50% yeast extract in the original medium for succinic acid production.
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, China.
authors.forename: Xuefei
authors.initials: X
authors.lastname: Bai
authors.forename: Kequan
authors.initials: K
authors.lastname: Chen
authors.forename: Guizi
authors.initials: G
authors.lastname: Ye
authors.forename: Xiumei
authors.initials: X
authors.lastname: Huang
authors.forename: Jian
authors.initials: J
authors.lastname: Li
authors.forename: Min
authors.initials: M
authors.lastname: Jiang
mesh_terms: D000188:Actinobacillus / Q000378:metabolism*; D000693:Anaerobiosis; D003470:Culture Media / Q000494:pharmacology*; D005285:Fermentation; D007220:Industrial Waste; D019802:Succinic Acid / Q000378:metabolism*
chemical_list: D003470:Culture Media; D007220:Industrial Waste; D019802:Succinic Acid