title: Cytotoxic activity of some marine brown algae against cancer cell lines.
abstract: The aim of this study was to investigate the in vitro cytotoxic activity of total extract of MeOH (70%) and partition fractions of hexan, chloroform (CHCL3), ethylacetate (EtOAc) and MeOH-H2O of brown algae species (Sargassum swartzii, Cystoseira myrica, Colpomenia sinuosa) found in the Persian Gulf against in different cell lines including HT-29, Caco-2, T47D, MDA-MB468 and NIH 3T3 cell lines by MTT and AnnexinV-PI assay. The hexan fraction of S. swartzii and C. myrica showed selective cytotoxicity against proliferation of Caco-2 cells (IC50 < 100 μg/ml) T47D cell line (IC50<100 μg/ml), respectively. S. swartzii and C. myrica were also observed for increasing apoptosis in Caco-2 and T47D cells. Total extract and fractions of C. sinuosa did not show any significant cytotoxicity against the studied cell lines. MDA-MB468 cells were more sensitive to C. myrica than was T47D (IC50 99.9 ± 8.11 vs. 56.50' ± 0.88). This reflects an estrogen receptor independent mechanism for cytotoxicity of the extract. The IC50 of the hexan fraction of C. myrica on T47D parent cells was lower than it was on T47D-TR cells (IC50 99.9 ± 8.11 vs. 143.15 ± 7.80). This finding suggests a role for the MDR-1 in the development of possible future tolerance to the extract.
authors.affiliation: Department of Pharmacognosy and Pharmaceutical Science Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran 14155-6451, Iran.
authors.forename: Mahnaz
authors.initials: M
authors.lastname: Khanavi
authors.forename: Maryam
authors.initials: M
authors.lastname: Nabavi
authors.forename: Nargess
authors.initials: N
authors.lastname: Sadati
authors.forename: Mohammadreza
authors.initials: M
authors.lastname: Shams Ardekani
authors.forename: Jelve
authors.initials: J
authors.lastname: Sohrabipour
authors.forename: Seyed Mohammad B
authors.initials: SM
authors.lastname: Nabavi
authors.forename: Padideh
authors.initials: P
authors.lastname: Ghaeli
authors.forename: Seyed Nasser
authors.initials: SN
authors.lastname: Ostad
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D020552:Comet Assay; D004354:Drug Screening Assays, Antitumor / Q000379:methods; D006801:Humans; D020128:Inhibitory Concentration 50; D000459:Phaeophyceae / Q000737:chemistry* / Q000145:classification
chemical_list: D000970:Antineoplastic Agents