title: Involvement of the Shewanella oneidensis decaheme cytochrome MtrA in the periplasmic stability of the beta-barrel protein MtrB.
abstract: The Shewanella oneidensis outer membrane β-barrel protein MtrB is part of a membrane-spanning protein complex (MtrABC) which is necessary for dissimilatory iron reduction. Quantitative PCR, heterologous gene expression, and mutant studies indicated that MtrA is required for periplasmic stability of MtrB. DegP depletion compensated for this MtrA dependence.
authors.affiliation: Universität Freiburg, Institut Biologie II, Mikrobiologie, Schänzlestr. 1, D-79104 Freiburg, Germany.
authors.forename: Marcus
authors.initials: M
authors.lastname: Schicklberger
authors.forename: Clemens
authors.initials: C
authors.lastname: Bücking
authors.forename: Bjoern
authors.initials: B
authors.lastname: Schuetz
authors.forename: Heinrich
authors.initials: H
authors.lastname: Heide
authors.forename: Johannes
authors.initials: J
authors.lastname: Gescher
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics* / Q000378:metabolism*; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D015153:Blotting, Western; D004579:Electron Transport; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D055786:Gene Knockout Techniques; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D007501:Iron / Q000378:metabolism; D009154:Mutation; D010084:Oxidation-Reduction; D019897:Periplasm / Q000235:genetics / Q000378:metabolism*; D033903:Periplasmic Proteins / Q000235:genetics / Q000378:metabolism; D016133:Polymerase Chain Reaction; D055550:Protein Stability; D012697:Serine Endopeptidases / Q000235:genetics / Q000378:metabolism; D020592:Shewanella / Q000235:genetics* / Q000378:metabolism*
chemical_list: D018528:ATP-Binding Cassette Transporters; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D006360:Heat-Shock Proteins; C100938:MtrA protein, Bacteria; C116147:MtrB protein, Shewanella putrefaciens; D033903:Periplasmic Proteins; D007501:Iron; C061682:DegP protease; D012697:Serine Endopeptidases