title: [Effects of intrathecal injection of U0126 on the expression of phospho-CREB in spinal cord of morphine-induced withdrawal rats].
abstract: OBJECTIVE
  To explore effects of intrathecal injection of U0126 on morphine withdrawal response and the spinal Phospho-CREB expression in morphine-induced withdrawal rats.
  
  
  METHODS
  All the rats were divided into 5 groups: control group, dependence group, withdrawal group, U0126 group (5 microg, it) and DMSO group. Morphine withdrawal score, touch evoked agitation scores(TEA score), immunohistochemical and Western-blotting technique were used to evaluate morphine withdrawal response and the expression of Phospho-CREB in the spinal cord.
  
  
  RESULTS
  Intrathecal injection of MEK inhibitor U0126 significantly alleviated morphine withdrawal symptoms. Morphine withdrawal scores in U0126 group (22.5 +/- 4.09) were significantly lower than that of withdrawal group (28.6 +/- 4.89, P < 0.05). TEA score of withdrawal group was 13.5 +/- 2.55, which was significantly higher than that of U0126 group (10.0 +/- 2.76, P < 0.05). Phospho-CREB positive neurons in the spinal dorsal horn of withdrawal group were 380 +/- 71, which is higher than that of U0126 group (293 +/- 47, P < 0.05). Compared with withdrawal group, level of Phospho-CREB protein detected by Western blot in spinal cord of U0126 group was significantly lower.
  
  
  CONCLUSIONS
  MEK inhibitors U0126 could suppress expression of Phospho-CREB in the spinal cord.
authors.affiliation: Department of Anesthesiology, 1st Hospital of Huai'an, Affiliated Hospital of Nanjing Medical University, Huai'an 223300, China.
authors.forename: Hai-Lin
authors.initials: HL
authors.lastname: Liu
authors.forename: Jun-Li
authors.initials: JL
authors.lastname: Cao
authors.forename: Pei-Jing
authors.initials: PJ
authors.lastname: Dai
authors.forename: Guo-Long
authors.initials: GL
authors.lastname: Zheng
authors.forename: Ying-Ming
authors.initials: YM
authors.lastname: Zeng
mesh_terms: D000818:Animals; D002070:Butadienes / Q000494:pharmacology* / Q000627:therapeutic use; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism*; D007278:Injections, Spinal; D008297:Male; D009021:Morphine Dependence / Q000188:drug therapy / Q000378:metabolism*; D009570:Nitriles / Q000494:pharmacology* / Q000627:therapeutic use; D047428:Protein Kinase Inhibitors / Q000494:pharmacology* / Q000627:therapeutic use; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000187:drug effects / Q000378:metabolism; D013375:Substance Withdrawal Syndrome / Q000188:drug therapy / Q000378:metabolism*
chemical_list: D002070:Butadienes; D017362:Cyclic AMP Response Element-Binding Protein; D009570:Nitriles; D047428:Protein Kinase Inhibitors; C113580:U 0126