title: [Effect of inhibiting endoxin by antidigoxin antiserum on myocardial ischemia/reperfusion injury in rats].
abstract: OBJECTIVE
  To study the effect of antidigoxin antiserum on oxygen stress induced by myocardial ischemia/reperfusion (MI/R) injury in rats.
  
  
  METHODS
  Sprauge Dawley rats were submitted to ligate left anterior descending coronary artery 30 min followed by 45 min reperfusion. Experiment animals were randomly divided into seven groups including sham group, MI/R group, normal salina group, verapamil group and three antidigoxin antiserum groups from low to high dose. The left ventricular myocardial tissue sample of ischemia were processed and measured the level of endoxin and malondialdehyde (MDA), the activities of Na+, K(+) -ATPase and superoxin dismutase (SOD). The myocardia morphology was observed.
  
  
  RESULTS
  The levels of endoxin and MDA increased and the activities of Na+, K(+) -ATPase and MDA were inhibited significantly in MI/R and saline groups. Including verapamil group in comparison to MI/R and saline groups, MDA level decreased and SOD activities partly reserved, meanwhile, only in three antidigoxin antiserum groups, the myocardial endoxin level was remarkably decreased, Na+, K(+) -ATPase activities were drastically increased. The myocardial histological morphology was significantly improved.
  
  
  CONCLUSIONS
  Antidigoxin antiserum, an endoxin mutual clone antibody, had the effect of attenuating the damage of oxygen free radicals induced by MI/R via to antagonizing the inhibition effect of endoxin on myocardial membrane Na+, K(+) -ATPase activities.
authors.affiliation: Department of Geriatric Cardiology, Yijishan Hospital, Wannan Medical College, Wuhu 241001, China.
authors.forename: De-Guo
authors.initials: DG
authors.lastname: Wang
authors.forename: He-Gui
authors.initials: HG
authors.lastname: Wang
authors.forename: Yong-Sheng
authors.initials: YS
authors.lastname: Ke
authors.forename: Shang-Yin
authors.initials: SY
authors.lastname: Yang
mesh_terms: D000818:Animals; D002298:Cardenolides / Q000037:antagonists & inhibitors*; D004077:Digoxin / Q000494:pharmacology*; D007106:Immune Sera / Q000494:pharmacology*; D008315:Malondialdehyde / Q000032:analysis; D015425:Myocardial Reperfusion; D015428:Myocardial Reperfusion Injury / Q000503:physiopathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012503:Saponins / Q000037:antagonists & inhibitors*; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D002298:Cardenolides; D007106:Immune Sera; D012503:Saponins; C034504:digoxin-like factors; D008315:Malondialdehyde; D004077:Digoxin; D013482:Superoxide Dismutase; D000254:Sodium-Potassium-Exchanging ATPase