title: [Research on induced differentiation of human bone marrow mesenchymal stem cells into vascular endothelial cells].
abstract: OBJECTIVE
  To analyze gene expression difference between human mesenchymal stem cells and umbilical vein endothelial cells, to discuss the feasibility of inducing hMSCs to differentiate into endothelial cells through in vitro gene transfection as well as the use and prospective as a seeding cell source of vascular tissue engineering.
  
  
  METHODS
  hMSCs and hUVECs were isolated, expanded in culture, and characterized by flow-cytometry, immunocytochemistry, immunofluorescence and transmission electron microscopy (TEM). Differential analysis of gene expression profiles between them was performed by Biostar H-40 cDNA microarrays. The properties of VEGF 165 transfected were also detected with RT-PCR, ELISA et al.
  
  
  RESULTS
  Nearly 86% genes were coexpressed in both cells and hMSCs expressed typical endothelial antigen marker of EC. After VEGF165 transfection, hMSCs (or committed hMSCs) were positive for CD31. To different extent, the expression of CD44 was down regulated and CD34, FVIIIAg, Flt-1 up regulated.
  
  
  CONCLUSIONS
  Generation of functional EC or tissue engineered blood vessels from human MSCs is feasible utilizing an in vitro environment gene transfection.
authors.affiliation: Key Laboratory of Pathobiology, Jilin University, Changchun 130021, China.
authors.forename: Dan-Dan
authors.initials: DD
authors.lastname: Liu
authors.forename: Yue-Zeng
authors.initials: YZ
authors.lastname: Wang
authors.forename: Dong-Hai
authors.initials: DH
authors.lastname: Zhao
authors.forename: Yu-Lin
authors.initials: YL
authors.lastname: Li
mesh_terms: D001854:Bone Marrow Cells / Q000166:cytology*; D002454:Cell Differentiation; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology*; D004730:Endothelium, Vascular / Q000166:cytology*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D014162:Transfection; D042461:Vascular Endothelial Growth Factor A / Q000008:administration & dosage
chemical_list: C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A