title: [Studies on effects and mechanism of water immersion restraint stress on bile secretion in rats].
abstract: OBJECTIVE
  To probe into the operation mechanism of stress, through the studies on the effects of bile secretion in rats at the condition of water immersion restraint.
  
  
  METHODS
  The animals were divided into six groups (n=8): Group A: restraint alone under room temperature + saline; Group B: water immersion restraint + saline; Group C: restraint alone under room temperature + Atropine; Group D: water immersion restraint + Atropine; Group E: restraint alone under room temperature + Phentolamine; F group: water immersion restraint + Phentolamine.
  
  
  RESULTS
  Compared with group A, the capacity of bile secretion in group B decreased significantly (P < 0.05), changes of bile increased remarkably (P < 0.01), but there were no significant decreases on the capacity of bile secretion in group C (P > 0.05) compared with A, Group C only decreased appreciably. Compared with group A, the capacity of bile secretion in group E decreased appreciably (P < 0.05). Compared with group B, the capacity of bile secretion in group D decreased significantly (P < 0.05), pH of bile had no significant changes in group D. Compared with group B, the capacity of bile secretion in group F decreased significantly (P < 0.05), pH of bile had no significant changes in group F. Compared with group D, the capacity of bile secretion and pH of bile in group F had no significant changes.
  
  
  CONCLUSIONS
  Water immersion restraint stress inhibited evidently on the capacity of bile secretion, and the capacity of bile secretion in water immersion groups decreased significantly, moreover pH of bile increased greatly. At the condition of restraint alone under room temperature, vagus and sympathetic nerve had no significant effects on the bile secretion, but they played important roles in decreases of bile secretion evidently induced by water immersion restraint stress in rats (P < 0.05).
authors.affiliation: Chinese Women College, Shandong Branch, Jinan 250002, China.
authors.forename: Shu-xin
authors.initials: SX
authors.lastname: Yao
authors.forename: Hong-bin
authors.initials: HB
authors.lastname: Ai
mesh_terms: D000818:Animals; D001646:Bile / Q000378:metabolism*; D007101:Immersion; D008099:Liver / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012149:Restraint, Physical; D013312:Stress, Physiological