title: [The effects of formaldehyde on ovarian histopathology structure, oocyte ultrastructure and apoptosis of female rats].
abstract: OBJECTIVE
  To study the effects of formaldehyde on ovarian histopathology structure, oocyte ultrastructure and apoptosis of female rats.
  
  
  METHODS
  Healthy female rats were injected intraperitoneally with different doses of formaldehyde (20.0, 2.0, 0.2 mg/kg) continuously for 14 days. These mice were killed on the 14th day. Morphological changes of ovary cells were observed by light microscopy and ultrastructure of oocyte were observed by electron microscope. The oocyte apoptosis was determined by terminal deoxynucleotidyltransferase-mediated dUTP nick-end labelling (TUNEL) staining.
  
  
  RESULTS
  By light microscopy, pathological structure changes in ovarian of the female rat after treatment with formaldehyde, and the change in the rat ovarian increased with dose. By electron microscope oocyte ultrastructure changes in ovarian of the female rat after treatment with formaldehyde too, and the changes in the mouse ovarian increased with dose. The results also showed that formaldehyde could cause the apoptosis in the female mouse ovarian compared with the control. And in the middle and high doses, the difference between the apoptosis rate is distinctive.
  
  
  CONCLUSIONS
  Formaldehyde has adverse effects on ovarian histopathology structure, oocyte ultrastructure of female rats and it can cause the apoptosis of oocyte.
authors.affiliation: Department of Obstetrics and Gynecology, Xiangya Hospital, Central South University, Changsha, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Guo-qing
authors.forename: Tian
authors.initials: T
authors.lastname: Yan
authors.forename: Jin
authors.initials: J
authors.lastname: Ou
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000276:immunology; D005260:Female; D005557:Formaldehyde / Q000494:pharmacology*; D009865:Oocytes / Q000187:drug effects* / Q000648:ultrastructure; D006080:Ovarian Follicle / Q000000981:diagnostic imaging / Q000187:drug effects*; D010053:Ovary / Q000187:drug effects* / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D014463:Ultrasonography
chemical_list: D005557:Formaldehyde