title: [Expression of matrix metalloproteinase-2,matrix metalloproteinase-9 and vascular endothdial growth factor in retinoblastoma and its clinical significance].
abstract: OBJECTIVE
  To study the expression of matrix metalloproteinase-2(MMP-2), matrix metalloproteinase-9(MMP-9) and vascular endothelial growth factor(VEGF) in retinoblastoma (RB) and its relationship with the differentiation and optic nerve infiltration of RB.
  
  
  METHODS
  Immunohistochemical technique was used to detect the protein expression of MMP-2, MMP-9 and VEGF in 40 RB cases.
  
  
  RESULTS
  The MMP-2, MMP-9 protein and VEGF protein expression were detected as positive in 52.5%, 57.5% and 72.5% of 40 RB cases respectively. The expression of MMP-2, MMP-9 and VEGF was significantly higher in the undifferentiated pattern than in the rosetted pattern(p<0.05); and the expression of MMP-2, MMP-9 and VEGF was significantly higher in tumors with optic nerve invasion than in those without optic nerve invasion(p<0.05). In RB, the expression of MMP-2, MMP-9 had a positive correlation with the expression of VEGF.
  
  
  CONCLUSIONS
  In RB, high expression of MMP-2, MMP-9 and VEGF is related to the invasion and metastasis of RB. The expression of MMP-2, MMP-9 is positively correlated with the expression of VEGF. The expression of MMP-2, MMP-9 and VEGF may act as a biological marker for the invasion and metastasis of RB. The method may help screen and identify the cases with high risk for metastasis and to predict the prognosis of patients.
authors.affiliation: Huaian Hospital of Xuzhou Medical College,Huaian 223002,China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhou
authors.forename: Jia
authors.initials: J
authors.lastname: Xu
authors.forename: Jianfang
authors.initials: J
authors.lastname: Kang
mesh_terms: D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; D012175:Retinoblastoma; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D042461:Vascular Endothelial Growth Factor A; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9