title: [Effects of injectio Salvia miltiorrhiza on gentamicin-induced free radical formation in guinea pig cochlea].
abstract: OBJECTIVE
  To investigate the effects of injectio Salvia Miltiorrhiza (SM) on gentamicin (GM)-induced free radical formation in guinea pig cochlea, and to explore possible mechanisms on GM-induced ototoxicity.
  
  
  METHODS
  Biochemical assays of superoxide dismutase (SOD) activity and malondialdehyde (MDA) content in guinea pig cochlea, combined with auditory brainstem response (ABR) measurement and transmission electron microscopic observation were used in this investigation.
  
  
  RESULTS
  SOD activity was significantly declined while MDA content was distinctly increased in cochlear tissues after GM injection (P < 0.01). Moreover, they were well correlated with auditory function damage (|r| > 0.7). Co-treatment with SM evidently enhanced SOD activity and decreased MDA content (P < 0.01, P < 0.05). Furthermore, auditory function was markedly ameliorated. Morphological changes of cochlea were consistent with those of hearing function.
  
  
  CONCLUSIONS
  Lipid peroxidation elicited by free radical was involved in GM-induced cochleotoxicity. SM might enhance SOD activity and prevent lipid peroxidation. As the result it might alleviate GM ototoxicity, and improve auditory function.
authors.affiliation: Department of Physiology, Jinzhou Medical College, Jinzhou 121001, China.
authors.forename: Ai-Mei
authors.initials: AM
authors.lastname: Wang
authors.forename: Hao
authors.initials: H
authors.lastname: Tang
authors.forename: Jing
authors.initials: J
authors.lastname: Shen
authors.forename: Cheng
authors.initials: C
authors.lastname: Cui
mesh_terms: D000818:Animals; D003051:Cochlea / Q000187:drug effects* / Q000378:metabolism*; D005609:Free Radicals / Q000378:metabolism; D005839:Gentamicins / Q000633:toxicity*; D006168:Guinea Pigs; D015227:Lipid Peroxidation; D008315:Malondialdehyde / Q000032:analysis; D027561:Salvia miltiorrhiza; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D005609:Free Radicals; D005839:Gentamicins; D008315:Malondialdehyde; D013482:Superoxide Dismutase