title: [Protective effect of nitric oxide on gastric mucosa and its relationship to the acid secretion of gastric parietal cells under stress in rats].
abstract: OBJECTIVE
  To demonstrate the protective effect of nitric oxide (NO) on gastric mucosa and its relationship to the acid secretion of parietal cells under stress in rats.
  
  
  METHODS
  Water immersion-restraint stress (WRS) model in SD rats was performed. The gastric mucosal ulcer index (UI), NO contents in gastric mucosa and H+, K(+) -ATPase activity of parietal cells were measured. The effects of N(G)-nitro-L-arginine methyl ester(L-NAME) and L-arginine (L-Arg) on the H+, K(+)-ATPase activity of parietal cells and stress-induced gastric mucosal lesion were observed.
  
  
  RESULTS
  L-NAME pretreatment decreased NO contents in gastric mucosa, activated H+, K(+) -ATPase activity of parietal cells and aggravated gastric mucosal lesion, whereas L-Arg pretreatment increased NO contents, inhibited H+, K(+) -ATPase activity and significantly ameliorated stress-induced gastric mucosal lesion.
  
  
  CONCLUSIONS
  Endogenous nitric oxide plays an important role in protecting gastric mucosa from stress-induced lesion by inhibiting H+, K(+) -ATPase activity of parietal cells.
authors.affiliation: Medical College, Jiaxing College, Jiaxing 31400, China.
authors.forename: Guo-Ming
authors.initials: GM
authors.lastname: Lu
authors.forename: Yu-Mei
authors.initials: YM
authors.lastname: Li
authors.forename: Lian-Jun
authors.initials: LJ
authors.lastname: Guo
authors.forename: Ming
authors.initials: M
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001120:Arginine / Q000378:metabolism; D005744:Gastric Acid / Q000378:metabolism*; D005753:Gastric Mucosa / Q000378:metabolism*; D017506:H(+)-K(+)-Exchanging ATPase / Q000378:metabolism*; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000378:metabolism; D018384:Oxidative Stress; D010295:Parietal Cells, Gastric / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013276:Stomach Ulcer / Q000378:metabolism / Q000473:pathology; D013312:Stress, Physiological
chemical_list: D009569:Nitric Oxide; D001120:Arginine; D019001:Nitric Oxide Synthase; D017506:H(+)-K(+)-Exchanging ATPase