title: Mining recent brain proteomic databases for ion channel phosphosite nuggets.
abstract: Voltage-gated ion channels underlie electrical activity of neurons and are dynamically regulated by diverse cell signaling pathways that alter their phosphorylation state. Recent global mass spectrometric-based analyses of the mouse brain phosphoproteome have yielded a treasure trove of new data as to the extent and nature of phosphorylation of numerous ion channel principal or α subunits in mammalian brain. Here we compile and review data on 347 phosphorylation sites (261 unique) on 42 different voltage-gated ion channel α subunits that were identified in these recent studies. Researchers in the ion channel field can now begin to explore the role of these novel in vivo phosphorylation sites in the dynamic regulation of the localization, activity, and expression of brain ion channels through multisite phosphorylation of their principal subunits.
authors.affiliation: Department of Neurobiology, Physiology, and Behavior, University of California, Davis, Davis, CA 95616, USA.
authors.forename: Oscar
authors.initials: O
authors.lastname: Cerda
authors.forename: Je-Hyun
authors.initials: JH
authors.lastname: Baek
authors.forename: James S
authors.initials: JS
authors.lastname: Trimmer
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D057225:Data Mining / Q000379:methods*; D030562:Databases, Protein; D006801:Humans; D007473:Ion Channels / Q000235:genetics / Q000378:metabolism*; D013058:Mass Spectrometry / Q000379:methods; D009474:Neurons / Q000378:metabolism*; D010750:Phosphoproteins / Q000378:metabolism*; D010766:Phosphorylation; D015398:Signal Transduction; D050994:Voltage-Dependent Anion Channels / Q000235:genetics / Q000378:metabolism
chemical_list: D007473:Ion Channels; D010750:Phosphoproteins; D050994:Voltage-Dependent Anion Channels