title: NOS antagonism using viral vectors as an experimental strategy: implications for in vivo studies of cardiovascular control and peripheral neuropathies.
abstract: Nitric oxide, a free gaseous signalling molecule, has attracted the attention of numerous biologists and has been implicated in the regulation of the cardiovascular, nervous and immune system. However, the cellular mechanisms mediating nitric oxide modulation remain unclear. Upregulation by gene over-expression or down-regulation by gene inactivation of nitric oxide synthase has generated quantitative changes in abundance thereby permitting functional insights. We have tested and proved that genetic nitric oxide synthase antagonism using viral vectors, particularly with dominant negative mutants and microRNA 30-based short hairpin RNA, is an efficient and effective experimental approach to manipulate nitric oxide synthase expression both in vitro and in vivo.
authors.affiliation: Department of Physiology and Pharmacology, University of Bristol, Bristol, UK. bh.Liu@bristol.ac.uk
authors.forename: Beihui
authors.initials: B
authors.lastname: Liu
authors.forename: James
authors.initials: J
authors.lastname: Hewinson
authors.forename: Haibo
authors.initials: H
authors.lastname: Xu
authors.forename: Francisco
authors.initials: F
authors.lastname: Montero
authors.forename: Carmen R
authors.initials: CR
authors.lastname: Sunico
authors.forename: Federico
authors.initials: F
authors.lastname: Portillo
authors.forename: Julian F R
authors.initials: JF
authors.lastname: Paton
authors.forename: Bernardo
authors.initials: B
authors.lastname: Moreno-López
authors.forename: Sergey
authors.initials: S
authors.lastname: Kasparov
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D002318:Cardiovascular Diseases / Q000201:enzymology / Q000235:genetics / Q000473:pathology* / Q000628:therapy*; D002460:Cell Line; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics*; D006801:Humans; D009569:Nitric Oxide / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors* / Q000235:genetics* / Q000378:metabolism; D010523:Peripheral Nervous System Diseases / Q000201:enzymology / Q000235:genetics / Q000473:pathology / Q000628:therapy*; D051381:Rats
chemical_list: D009569:Nitric Oxide; D019001:Nitric Oxide Synthase