title: A transcriptome screen in yeast identifies a novel assembly factor for the mitochondrial complex III.
abstract: Starting from a transcriptome based study of the spatio-temporal expression of yeast genes encoding mitochondrial proteins of unknown function, we have identified the gene BCA1 (YLR077W). A FISH analysis showed that the BCA1 mRNA co-localized with the mitochondrial network. Cellular fractionation revealed that Bca1 is bound to the mitochondrial inner-membrane and protrudes into the inter-membrane space. We show that Bca1 controls an early step in complex III assembly and that the supra-molecular organization of Bca1 is dependent upon the assembly level of complex III. Thus, Bca1 is a novel assembly factor for the respiratory complex III.
authors.affiliation: Centre de Génétique Moléculaire, CNRS FRE 3144, Avenue de la terrasse, Gif-sur-Yvette F-91190, France.
authors.forename: Lise
authors.initials: L
authors.lastname: Mathieu
authors.forename: Sophie
authors.initials: S
authors.lastname: Marsy
authors.forename: Yann
authors.initials: Y
authors.lastname: Saint-Georges
authors.forename: Claude
authors.initials: C
authors.lastname: Jacq
authors.forename: Geneviève
authors.initials: G
authors.lastname: Dujardin
mesh_terms: D014450:Electron Transport Complex III / Q000378:metabolism*; D020869:Gene Expression Profiling; D017404:In Situ Hybridization, Fluorescence; D051336:Mitochondrial Membranes / Q000737:chemistry; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism*; D055503:Protein Multimerization; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D024101:Mitochondrial Proteins; D029701:Saccharomyces cerevisiae Proteins; D014450:Electron Transport Complex III