title: In vitro assessment of the antiviral potential of trans-cinnamic acid, quercetin and morin against equid herpesvirus 1.
abstract: The antiviral activity of quercetin, morin and trans-cinnamic acid was evaluated in vitro against equid herpesvirus 1 (EHV-1) by determining the virucidal activity and using the time of addition assay to test inhibition of the viral replication cycle. The cytotoxicity of each substance was assessed using MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide]. Quercetin showed virucidal action and inhibition of the viral replication cycle at 0 and 1h. Morin showed potential virucidal and viral replication cycle inhibition at 0 h. Trans-cinnamic acid did not show virucidal activity but inhibited the viral replication cycle at -1 and 0 h. This study demonstrates the potential of these compounds as future antiviral candidates in relation to viruses of importance in veterinary medicine.
authors.affiliation: Molecular Animal Infectology Laboratory, Applied Biotechnology Institute (BIOAGRO), Federal University of Vicosa, Vicosa 36570-000, Brazil.
authors.forename: H D
authors.initials: HD
authors.lastname: Gravina
authors.forename: N F
authors.initials: NF
authors.lastname: Tafuri
authors.forename: A
authors.initials: A
authors.lastname: Silva Júnior
authors.forename: J L R
authors.initials: JL
authors.lastname: Fietto
authors.forename: T T
authors.initials: TT
authors.lastname: Oliveira
authors.forename: M A N
authors.initials: MA
authors.lastname: Diaz
authors.forename: M R
authors.initials: MR
authors.lastname: Almeida
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D002522:Chlorocebus aethiops; D002934:Cinnamates / Q000494:pharmacology*; D005419:Flavonoids / Q000494:pharmacology*; D004861:Herpesvirus 1, Equid / Q000187:drug effects*; D011794:Quercetin / Q000494:pharmacology*; D014709:Vero Cells; D047309:Flavones
chemical_list: D000998:Antiviral Agents; D002934:Cinnamates; D005419:Flavonoids; D011794:Quercetin; C029010:cinnamic acid; C008548:morin; D047309:Flavones