title: Linear B-cell epitope mapping of neuraminidases of the 2009 A H1N1 viruses based on immunoinformatics.
abstract: The 2009 A H1N1 viruses have spread throughout the world, as the viral neuraminidase (NA) is a receptor-destroying enzyme, removing sialic acid from carbohydrate chains attached to NA, and releasing the viruses from infected cells. In this study, the NA genes of Guangdong viruses were sequenced, then their B-cell epitopes were predicted, screened and assessed based on immunoinformatics. The antisera were raised in rabbits against five linear synthetic peptides spanning the NA protein of 2009 A H1N1. Five peptides, LR17, SS12, DP9, DS11 and DI14, respectively, are capable of eliciting neutralizing antibodies against 2009 A H1N1 in the in vitro microneutralization assay. DI14 was identified to be particularly potent in eliciting a neutralizing antibody titer comparable to that obtained with a whole virion-immunized serum. Immunization of rabbit with either five peptides triggered a 2009 A H1N1-specific antibody response as high as that obtained with the whole virion as immunogen. Alignment with databases showed that the amino acid residues of five epitope peptides are highly conserved among the NA sequences of 2009 A H1N1 strains isolated from the world. Altogether, these data indicate that LR17, SS12, DP9, DS11 and DI14 represent a promising candidate for an effective synthetic peptide-based vaccine against 2009 A H1N1 viruses.
authors.affiliation: Key Laboratory for Emergency Pathogen Detection, Center for Disease Control and Prevention of Guangdong Province, Guangzhou 510300, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Huang
authors.forename: Yuansheng
authors.initials: Y
authors.lastname: Xu
authors.forename: Hanzhong
authors.initials: H
authors.lastname: Ni
authors.forename: Jing
authors.initials: J
authors.lastname: Zhong
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.forename: Songnuan
authors.initials: S
authors.lastname: Tan
authors.forename: De
authors.initials: D
authors.lastname: Wu
authors.forename: Baoyun
authors.initials: B
authors.lastname: Qiu
authors.forename: Dawei
authors.initials: D
authors.lastname: Guan
authors.forename: Miaoheng
authors.initials: M
authors.lastname: Wen
authors.forename: Jin
authors.initials: J
authors.lastname: Yan
authors.forename: Yonghui
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood / Q000276:immunology; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology; D019295:Computational Biology / Q000379:methods*; D018604:Epitope Mapping; D018985:Epitopes, B-Lymphocyte / Q000235:genetics / Q000276:immunology*; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics / Q000276:immunology*; D009439:Neuraminidase / Q000235:genetics / Q000276:immunology*; D009500:Neutralization Tests; D011817:Rabbits; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology*
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D018985:Epitopes, B-Lymphocyte; D014764:Viral Proteins; C487630:NA protein, influenza A virus; D009439:Neuraminidase