title: [Genomic profiling by comparative genomic hybridization: analysis of ten enucleated uveal melanoma cases].
abstract: OBJECTIVE
  To detect major chromosomal aberrations from enucleated uveal melanoma and relate them to hepatic metastasis and survival.
  
  
  METHODS
  Ten uveal melanomas enucleated between 2005 and 2008 in the Lyon Croix-Rousse Hospital were retrospectively analyzed using a 19 000-clone comparative genomic hybridization microarray.
  
  
  RESULTS
  The most frequent imbalances were the loss of chromosome 3 (8/10), gain of the 8q arm (7/10) or the entire chromosome 8 (2/10), and gain of the 6p arm (2/10). Most metastatic tumors (6/7) and all cases of death (5/5) concerned melanoma with monosomy 3 and gain of the 8q arm.
  
  
  CONCLUSIONS
  Genome-wide array comparative genomic hybridization is a reliable tool for identifying uveal melanoma genomic imbalances. Gains of the 8q arm with monosomy 3 are frequent and are strongly associated with poor outcome. Gains of the 6p arm are rare and have a better prognosis. There is a mutually exclusive relationship between monosomy 3 and chromosome 6 abnormalities in our study. These results confirm previously published reports.
authors.affiliation: Service d'ophtalmologie, hôpital de la Croix-Rousse, 103, Grande rue de la Croix-Rousse, 69317 Lyon cedex 04, France. niiil@free.fr
authors.forename: N
authors.initials: N
authors.lastname: Abi-Ayad
authors.forename: J
authors.initials: J
authors.lastname: Couturier
authors.forename: M
authors.initials: M
authors.lastname: Devouassoux-Shisheboran
authors.forename: J-D
authors.initials: JD
authors.lastname: Grange
authors.forename: L
authors.initials: L
authors.lastname: Kodjikian
authors.forename: A
authors.initials: A
authors.lastname: Calender
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D055028:Comparative Genomic Hybridization; D015353:Eye Enucleation; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D008545:Melanoma / Q000235:genetics*; D008875:Middle Aged; D012189:Retrospective Studies; D014604:Uveal Neoplasms / Q000235:genetics*