title: Neuropeptide FF receptor antagonist, RF9, attenuates the fever induced by central injection of LPS in mice.
abstract: The endogenous opioid system has been found to be involved in the fever caused by lipopolysaccharide (LPS). Neuropeptide FF (NPFF, FLFQPQRF-NH(2)) is an endogenous peptide known to modulate opioid activity, mainly in the central nervous system. Therefore, those data suggested a link between LPS-induced fever and NPFF systems. Using a model of acute neuroinflammation, we sought to determine the effects of NPFF systems on the fever induced by i.c.v. injection of LPS. Coinjected with different doses of NPFF (10 and 30 nmol), the fever of LPS (125 ng) was not modified. Interestingly, the selective NPFF receptors antagonist RF9 (30 nmol) injected into the third ventricle failed to induce significant effect, but it decreased the fever of LPS (125 ng) after cerebral administration in mice. These results suggest that NPFF receptors activation is required for LPS to produce fever. This interaction is the first evidence that NPFF systems participate in the control of acute neuroinflammation in conscious animals.
authors.affiliation: Key Laboratory of Preclinical Study for New Drugs of Gansu Province, School of Basic Medical Sciences, and Institute of Biochemistry and Molecular Biology, School of Life Science, Lanzhou University, 222 Tian Shui South Road, Lanzhou 730000, PR China.
authors.forename: Yi-qing
authors.initials: YQ
authors.lastname: Wang
authors.forename: Sheng-bin
authors.initials: SB
authors.lastname: Wang
authors.forename: Jing-lin
authors.initials: JL
authors.lastname: Ma
authors.forename: Jia
authors.initials: J
authors.lastname: Guo
authors.forename: Quan
authors.initials: Q
authors.lastname: Fang
authors.forename: Tao
authors.initials: T
authors.lastname: Sun
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhuang
authors.forename: Rui
authors.initials: R
authors.lastname: Wang
mesh_terms: D000218:Adamantane / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use; D000818:Animals; D004151:Dipeptides / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D005334:Fever / Q000139:chemically induced / Q000188:drug therapy*; D007276:Injections, Intraventricular; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000633:toxicity*; D008297:Male; D051379:Mice; D018013:Receptors, Neuropeptide / Q000037:antagonists & inhibitors*
chemical_list: D004151:Dipeptides; D008070:Lipopolysaccharides; D018013:Receptors, Neuropeptide; C508361:adamantylcarbonyl-arginyl-phenylalaninamide; C083929:neuropeptide FF receptor; D000218:Adamantane