title: Antioxidant activities of total phenols of Prunella vulgaris L. in vitro and in tumor-bearing mice.
abstract: Prunella vulgaris L. (PV, Labiatae) is known as a self-heal herb. The different extracts of dried spikes were studied for the best antioxidant active compounds. The 60% ethanol extract (P-60) showed strong antioxidant activity based on the results of 2,2'-azino-di(3-ethylbenzthiazoline-6-sulfonic acid (ABTS˙+), 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) assay methods. High performance liquid chromatography (HPLC) and LC/MS analysis showed that the main active compounds in P-60 were phenols, such as caffeic acid, rosmarinic acid, rutin and quercetin. Total phenols were highly correlated with the antioxidant activity (R2=0.9988 in ABTS˙+; 0.6284 in DPPH and 0.9673 FRAP tests). P-60 could inhibit significantly the tumor growth in C57BL/6 mice. It can also been showed that increased superoxide dismutase (SOD) activity and decreased malondialdehyde (MDA) content in serum of tumor-bearing mice. These results suggested that P-60 extract had high antioxidant activity in vitro and in vivo and total phenols played an important role in antioxidant activity for inhibition of tumor growth.
authors.affiliation: Biotechnology Laboratory of Chinese Medicine, Macau University of Science and Technology, Macau, China. wenmoxiushi@163.com
authors.forename: Liang
authors.initials: L
authors.lastname: Feng
authors.forename: Xiaobin
authors.initials: X
authors.lastname: Jia
authors.forename: Mao-Mao
authors.initials: MM
authors.lastname: Zhu
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.forename: Feng
authors.initials: F
authors.lastname: Shi
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D045744:Cell Line, Tumor; D008315:Malondialdehyde / Q000097:blood; D051379:Mice; D009374:Neoplasms, Experimental / Q000097:blood / Q000188:drug therapy*; D010636:Phenols / Q000737:chemistry / Q000494:pharmacology; D031347:Prunella / Q000737:chemistry*; D013482:Superoxide Dismutase / Q000097:blood
chemical_list: D000970:Antineoplastic Agents; D000975:Antioxidants; D010636:Phenols; D008315:Malondialdehyde; D013482:Superoxide Dismutase