title: Development of a DNA vaccine against chicken anemia virus by using a bicistronic vector expressing VP1 and VP2 proteins of CAV.
abstract: In the present study, we describe the development of a DNA vaccine against chicken anemia virus. The VP1 and VP2 genes of CAV were amplified and cloned into pBudCE4.1 to construct two DNA vaccines, namely, pBudVP1 and pBudVP2-VP1. In vitro and in vivo studies showed that co-expression of VP1 with VP2 are required to induce significant levels of antibody against CAV. Subsequently, the vaccines were tested in 2-week-old SPF chickens. Chickens immunized with the DNA-plasmid pBudVP2-VP1 showed positive neutralizing antibody titer against CAV. Furthermore, VP1-specific proliferation induction of splenocytes and also high serum levels of Th1 cytokines, IL-2 and IFN-γ were detected in the pBudVP2-VP1-vaccinated chickens. These results suggest that the recombinant DNA plasmid co-expressing VP1 and VP2 can be used as a potential DNA vaccine against CAV.
authors.affiliation: Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
authors.forename: Hassan
authors.initials: H
authors.lastname: Moeini
authors.forename: Abdul Rahman
authors.initials: AR
authors.lastname: Omar
authors.forename: Raha Abdul
authors.initials: RA
authors.lastname: Rahim
authors.forename: Khatijah
authors.initials: K
authors.lastname: Yusoff
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D036022:Capsid Proteins / Q000235:genetics / Q000276:immunology*; D002460:Cell Line; D017727:Chicken anemia virus / Q000235:genetics / Q000276:immunology*; D018173:Circoviridae Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D016207:Cytokines / Q000097:blood; D000939:Epitopes / Q000276:immunology; D023061:Gene Order; D005822:Genetic Vectors / Q000235:genetics; D011201:Poultry Diseases / Q000276:immunology* / Q000517:prevention & control; D013154:Spleen / Q000276:immunology; D014611:Vaccination / Q000662:veterinary; D019444:Vaccines, DNA / Q000235:genetics / Q000276:immunology*; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D036022:Capsid Proteins; D016207:Cytokines; D000939:Epitopes; D019444:Vaccines, DNA; D014765:Viral Vaccines