title: DJ-1-Mediated protective effect of protocatechuic aldehyde against oxidative stress in SH-SY5Y cells.
abstract: DJ-1 was identified as a causal gene for a familial form of early onset Parkinson's disease (PD), park 7. DJ-1 plays roles in transcriptional regulation and the anti-oxidative stress reaction. In this study, we found that protocatechuic aldehyde (PAL), a traditional Chinese medicine compound, bound to DJ-1 in vitro and that PAL protected SH-SY5Y cells but not DJ-1-knockdown SH-SY5Y cells from oxidative stress-induced cell death, indicating that the protective effect of PAL is mediated by DJ-1. Furthermore, PAL inhibited production of reactive oxygen species and the inhibition was abated in DJ-1-knockdown cells. PAL increased and decreased phosphorylation of AKT and PTEN, respectively, in SH-SY5Y cells, suggesting that the AKT pathway is one of the specific signaling pathways in PAL-induced neuroprotection. Moreover, PAL prevented superfluous oxidation of cysteine 106 of DJ-1, an essential amino acid for DJ-1's function. The present study demonstrates that PAL has potential neuroprotective effects through DJ-1.
authors.affiliation: Hokkaido University, Japan.
authors.forename: Jian-Wei
authors.initials: JW
authors.lastname: Gao
authors.forename: Takuya
authors.initials: T
authors.lastname: Yamane
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Maita
authors.forename: Shizuma
authors.initials: S
authors.lastname: Ishikawa
authors.forename: Sanae M M
authors.initials: SM
authors.lastname: Iguchi-Ariga
authors.forename: Xiao-Ping
authors.initials: XP
authors.lastname: Pu
authors.forename: Hiroyoshi
authors.initials: H
authors.lastname: Ariga
mesh_terms: D000077329:Agammaglobulinaemia Tyrosine Kinase; D001547:Benzaldehydes / Q000378:metabolism / Q000494:pharmacology*; D002396:Catechols / Q000378:metabolism / Q000494:pharmacology*; D016923:Cell Death / Q000187:drug effects; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism / Q000502:physiology*; D009447:Neuroblastoma / Q000473:pathology*; D018696:Neuroprotective Agents; D015513:Oncogene Proteins / Q000378:metabolism / Q000502:physiology*; D018384:Oxidative Stress / Q000187:drug effects*; D051059:PTEN Phosphohydrolase / Q000378:metabolism; D010300:Parkinson Disease / Q000235:genetics; D010766:Phosphorylation; D011485:Protein Binding; D000071617:Protein Deglycase DJ-1; D011505:Protein-Tyrosine Kinases / Q000378:metabolism / Q000502:physiology; D015398:Signal Transduction; D014407:Tumor Cells, Cultured
chemical_list: D001547:Benzaldehydes; D002396:Catechols; D047908:Intracellular Signaling Peptides and Proteins; D018696:Neuroprotective Agents; D015513:Oncogene Proteins; C005581:protocatechualdehyde; D011505:Protein-Tyrosine Kinases; D000077329:Agammaglobulinaemia Tyrosine Kinase; C105131:PARK7 protein, human; D000071617:Protein Deglycase DJ-1; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human