title: Determination of cyanuric acid in milk powder by anion-exchange chromatography.
abstract: An anion-exchange chromatographic method has been developed for simultaneous analysis of cyanuric acid (CA) and five inorganic anions (F(-), Cl(-), NO(3)(-), HPO(4)(2-) and SO(4)(2-)) in milk powder. The separation was achieved within 15 min on an anion-exchange column with simple elution of Na(2)CO(3)/NaHCO(3) buffer as mobile phase. Furthermore, the effect of the total concentration of Na(2)CO(3)/NaHCO(3) buffer on the retention of five inorganic anions was more obvious than that on CA retention, which indicated that CA retention on anion-exchange column depends not only on anion-exchange interaction but also on hydrophobic interactions between CA and anion-exchange column. The linear range of the calibration curve for CA was 0.1-100 mg L(-1). The detection limit calculated at S/N = 3 was 0.083 mg L(-1). The method was successfully applied to the analysis of CA in milk powder.
authors.affiliation: Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology, Ministry of Education, Department of Chemistry, Tsinghua University, Beijing, P. R. China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.forename: Shengjie
authors.initials: S
authors.lastname: Hou
authors.forename: Mingyu
authors.initials: M
authors.lastname: Ding
authors.forename: Jinghua
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000838:Anions / Q000032:analysis; D002138:Calibration; D002852:Chromatography, Ion Exchange / Q000379:methods* / Q000592:standards; D057927:Hydrophobic and Hydrophilic Interactions; D057230:Limit of Detection; D008722:Methods; D008892:Milk / Q000737:chemistry*; D011208:Powders / Q000032:analysis; D014227:Triazines / Q000032:analysis*
chemical_list: D000838:Anions; D011208:Powders; D014227:Triazines; C004632:cyanuric acid