title: Exchanging the as-1-like element of the PR-1 promoter by the as-1 element of the CaMV 35S promoter abolishes salicylic acid responsiveness and regulation by NPR1 and SNI1.
abstract: The plant defense hormone salicylic acid (SA) activates gene expression through a number of different mechanisms. In Arabidopsis thaliana, the SA-induced PATHOGENESIS RELATED (PR)-1 promoter is regulated through TGA transcription factors binding to the two TGACG motifs of the so called as-1 (activation sequence-1)-like element which is located between base pair positions -665 and -641. Activation is mediated by the transcriptional co-activator NPR1 (NON EXPRESSOR OF PR GENES1), which physically interacts with TGA factors. Moreover, the promoter is under the control of the negative regulator SNI1 (SUPPRESSOR OF NPR1, INDUCIBLE1). We have recently reported that SNI1-mediated repression of basal promoter activities and NPR1-dependent induction are maintained in a truncated PR-1 promoter that contains sequences between -816 and -573 upstream of the -68 promoter region. In this addendum, we report that the expression characteristics of this truncated PR-1 promoter is changed profoundly when its as-1-like element is replaced by the as-1 element of Cauliflower Mosaic Virus 35S promoter which also contains two TGACG motifs. The resulting chimeric promoter showed high constitutive activity that was independent from SA, NPR1 and SNI1. Thus, the configuration of two TGA binding sites within the PR-1 promoter determines whether NPR1 can induce and whether SNI1 can repress the promoter.
authors.affiliation: Albrecht-von-Haller-Institut für Pflanzenwissenschaften, Georg-August-Universität Göttingen, Göttingen, Germany.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Pape
authors.forename: Corinna
authors.initials: C
authors.lastname: Thurow
authors.forename: Christiane
authors.initials: C
authors.lastname: Gatz
mesh_terms: D029681:Arabidopsis Proteins / Q000235:genetics* / Q000502:physiology*; D017796:Caulimovirus / Q000235:genetics*; D009687:Nuclear Proteins / Q000502:physiology*; D011401:Promoter Regions, Genetic; D020156:Salicylic Acid / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; C105496:NPR1 protein, Arabidopsis; D009687:Nuclear Proteins; C121026:SNI1 protein, Arabidopsis; C077262:PR-1 protein, Arabidopsis; D020156:Salicylic Acid