title: Plasmodesmal receptor-like kinases identified through analysis of rice cell wall extracted proteins.
abstract: In plants, plasmodesmata (PD) are intercellular channels that function in both metabolite exchange and the transport of proteins and RNAs. Currently, many of the PD structural and regulatory components remain to be elucidated. Receptor-like kinases (RLKs) belonging to a notably expanded protein family in plants compared to the animal kingdom have been shown to play important roles in plant growth, development, pathogen resistance, and cell death. In this study, cell biological approaches were used to identify potential PD-associated RLK proteins among proteins contained within cell walls isolated from rice callus cultured cells. A total of 15 rice RLKs were investigated to determine their subcellular localization, using an Agrobacterium-mediated transient expression system. Of these six PD-associated RLKs were identified based on their co-localization with a viral movement protein that served as a PD marker, plasmolysis experiments, and subcellular localization at points of wall contact between spongy mesophyll cells. These findings suggest potential PD functions in apoplasmic signaling in response to environmental stimuli and developmental inputs.
authors.affiliation: Division of Applied Life Science (BK21 Program), Environmental Biotechnology National Core Research Center, Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju, 660-701, Republic of Korea.
authors.forename: Yeonhwa
authors.initials: Y
authors.lastname: Jo
authors.forename: Won Kyong
authors.initials: WK
authors.lastname: Cho
authors.forename: Yeonggil
authors.initials: Y
authors.lastname: Rim
authors.forename: Juyeon
authors.initials: J
authors.lastname: Moon
authors.forename: Xiong-Yan
authors.initials: XY
authors.lastname: Chen
authors.forename: Hyosub
authors.initials: H
authors.lastname: Chu
authors.forename: Cha Young
authors.initials: CY
authors.lastname: Kim
authors.forename: Zee-Yong
authors.initials: ZY
authors.lastname: Park
authors.forename: William J
authors.initials: WJ
authors.lastname: Lucas
authors.forename: Jae-Yean
authors.initials: JY
authors.lastname: Kim
mesh_terms: D002462:Cell Membrane / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D002473:Cell Wall / Q000378:metabolism*; D017124:Conserved Sequence; D004721:Endoplasmic Reticulum / Q000378:metabolism; D058503:Mesophyll Cells / Q000378:metabolism / Q000648:ultrastructure; D012275:Oryza / Q000378:metabolism*; D053830:Plant Viral Movement Proteins / Q000378:metabolism; D031425:Plasmodesmata / Q000378:metabolism*; D011494:Protein Kinases / Q000378:metabolism*; D021382:Protein Sorting Signals; D017434:Protein Structure, Tertiary
chemical_list: D053830:Plant Viral Movement Proteins; D021382:Protein Sorting Signals; D011494:Protein Kinases