title: Phosphorylation of ribosomal proteins influences subunit association and translation of poly (U) in Streptomyces coelicolor.
abstract: The occurrence of phosphorylated proteins in ribosomes of Streptomyces coelicolor was investigated. Little is known about which biological functions these posttranslational modifications might fulfil. A protein kinase associated with ribosomes phosphorylated six ribosomal proteins of the small subunit (S3, S4, S12, S13, S14 and S18) and seven ribosomal proteins of the large subunit (L2, L3, L7/L12, L16, L17, L23 and L27). The ribosomal proteins were phosphorylated mainly on the Ser/Thr residues. Phosphorylation of the ribosomal proteins influences ribosomal subunits association. Ribosomes with phosphorylated proteins were used to examine poly (U) translation activity. Phosphorylation induced about 50% decrease in polyphenylalanine synthesis. After preincubation of ribosomes with alkaline phosphatase the activity of ribosomes was greatly restored. Small differences were observed between phosphorylated and unphosphorylated ribosomes in the kinetic parameters of the binding of Phe-tRNA to the A-site of poly (U) programmed ribosomes, suggesting that the initial binding of Phe-tRNA is not significantly affected by phosphorylation. On contrary, the rate of peptidyl transferase was about two-fold lower than that in unphosphorylated ribosomes. The data presented demonstrate that phosphorylation of ribosomal proteins affects critical steps of protein synthesis.
authors.affiliation: Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague 4, Videnská 1083, Czech Republic. mikulik@biomed.cas.cz
authors.forename: Karel
authors.initials: K
authors.lastname: Mikulík
authors.forename: Jan
authors.initials: J
authors.lastname: Bobek
authors.forename: Alice
authors.initials: A
authors.lastname: Ziková
authors.forename: Magdalena
authors.initials: M
authors.lastname: Smětáková
authors.forename: Silvie
authors.initials: S
authors.lastname: Bezoušková
mesh_terms: D001665:Binding Sites; D010458:Peptidyl Transferases / Q000378:metabolism; D010766:Phosphorylation; D011072:Poly U / Q000235:genetics / Q000378:metabolism*; D012269:Ribosomal Proteins / Q000378:metabolism*; D054657:Ribosome Subunits / Q000378:metabolism*; D048372:Streptomyces coelicolor / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D046249:Transfer RNA Aminoacylation
chemical_list: D012269:Ribosomal Proteins; D011072:Poly U; D010458:Peptidyl Transferases