title: Effect of dynorphin A (1-17) on proliferation and IL-2, IL-4, IFN-γ production by peripheral blood mononuclears.
abstract: Dynorphin A (1-17) in concentrations of 10(-8)-10(-9) M inhibits phytohemagglutinin (2.5 μg/ml)-induced proliferative response of mononuclear fraction lymphocytes. In mitogen-stimulated cultures, 10(-8) M dynorphin A (1-17) stimulates the production of IL-4, inhibits the production of IL-2, and does not modify the production of IFN-γ. Nonselective κ-receptor antagonist naloxone and selective antagonist binaltorphimine hydrochloride abolish the inhibitory effects of both dynorphin A concentrations on the lymphocyte proliferative response. On the other hand, evaluation of the effect of κ-receptor blockade on the production of IL-2 and IL-4 showed that this effect depends on peptide concentration and antagonist type. Hence, the results attest to an important role of κ-receptors in modulation of functional activity of immune cells.
authors.affiliation: Institute of Ecology and Genetics of Microorganisms, Ural Division of the Russian Academy of Sciences, Perm; Perm State University, Russia. gein@iegm.ru
authors.forename: S V
authors.initials: SV
authors.lastname: Gein
authors.forename: A A
authors.initials: AA
authors.lastname: Siytchihin
mesh_terms: D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004399:Dynorphins / Q000494:pharmacology*; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism*; D007376:Interleukin-2 / Q000378:metabolism*; D015847:Interleukin-4 / Q000378:metabolism*; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*
chemical_list: D007376:Interleukin-2; D015847:Interleukin-4; D004399:Dynorphins; D007371:Interferon-gamma