title: [Protective effect of Naoyikang on the damage induced by glutamate in hippocampal neuron].
abstract: OBJECTIVE
  To investigate the effect of Naoyikang serum on the damage induced by glutamate in hippocampal neuron.
  
  
  METHODS
  Morphological observation, MTT assay and nuclear DNA-associated fluorescence with DAPI dye were applied to evaluate the viability of hippocampal neuron, immunocytochemistry and RT-PCR were used to determine the expression of PTEN.
  
  
  RESULTS
  A decreased viability and increased expression of PTEN were shown in hippocampal neuron in response to the treatment with glutamate. It was shown that the percentage of cell death and the expression of PTEN were reduced by the treatment with Naoyikang serum.
  
  
  CONCLUSIONS
  These results suggest that Naoyikang may prevent the toxicity of glutamate by suppressing the expression of PTEN.
authors.affiliation: Department of Pathophysiology, Basic Medical College of Nantong University, Nantong 226001, China.
authors.forename: Ya-E
authors.initials: YE
authors.lastname: Hu
authors.forename: Ai-Ling
authors.initials: AL
authors.lastname: Zhou
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhu
authors.forename: Jia-Hui
authors.initials: JH
authors.lastname: Mao
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: Shi
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D016923:Cell Death / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D018698:Glutamic Acid / Q000494:pharmacology*; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D018696:Neuroprotective Agents / Q000494:pharmacology*; D051059:PTEN Phosphohydrolase / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D044967:Serum
chemical_list: D004365:Drugs, Chinese Herbal; D018696:Neuroprotective Agents; D018698:Glutamic Acid; D051059:PTEN Phosphohydrolase