title: [Distribution and efficiency of pEGFP-N1 plasmid after its transfection in cardiomyocytes].
abstract: OBJECTIVE
  To study the distribution and efficiency of pEGFP-N1 plasmid after its transfection in cardiomyocytes.
  
  
  METHODS
  The neonatal rat cardiomyocytes were cultured. According to the different grow period of neonatal rat cardiomyocytes, the distribution and efficiency of pEGFP-N1 plasmid after its transfection in cardiomyocytes were studied.
  
  
  RESULTS
  The efficiency was significantly increased after pEGFP-N1 plasmid transfection in one-day cardiomyocytes. The distribution of EGFP was in the cytoplasm and nucleus.
  
  
  CONCLUSIONS
  The efficiency of pEGFP-N1 plasmid after its transfection in cardiomyocytes was related to the grow period of neonatal rat cardiomyocytes. The distribution of EGFP was in the cytoplasm and nucleus.
authors.affiliation: Institute of Health and Environmental Medicine, Academy of Military Medical Sciences, Tianjin 300050, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Feng
authors.forename: Wen-lin
authors.initials: WL
authors.lastname: Xiu
authors.forename: Rui
authors.initials: R
authors.lastname: Zhan
authors.forename: Ling-jia
authors.initials: LJ
authors.lastname: Qian
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D049452:Green Fluorescent Proteins / Q000235:genetics* / Q000378:metabolism*; D032383:Myocytes, Cardiac / Q000378:metabolism*; D010957:Plasmids; D051381:Rats; D017208:Rats, Wistar; D014162:Transfection
chemical_list: D049452:Green Fluorescent Proteins