title: Evidence of molecular alterations in the tumour suppressor gene WWOX in benign and malignant bone related lesions of the jaws.
abstract: WWOX is a tumour suppressor gene altered in various human neoplasms. Deletion of WWOX is associated with bone metabolic defects and development of osteosarcoma in mice. We hypothesized that alterations of this gene are associated with the development of benign and malignant mesenchymal bone related lesions of the jaws. We investigated WWOX mRNA by nested reverse transcription-PCR and direct sequencing and quantitative real-time PCR in two osteosarcoma, two fibrosarcoma, eight ossifying fibroma and two fibrous dysplasia fresh samples. Malignancy was associated with a decreased WWOX mRNA expression. Aberrant transcription pattern was found in five samples; however, the relative quantification (RQ) of the WWOX mRNA in such lesions was not different from those carrying only the wild-type. We provide new evidence of WWOX alterations in osteosarcomas and demonstrate for the first time alterations of this gene in fibrosarcomas as well as in ossifying fibromas of the jaws.
authors.affiliation: Department of Oral Surgery and Pathology, Universidade Federal de Minas Gerais, Av. Antonio Carlos, 6627 Belo Horizonte, MG Brazil, 31270901, Brazil.
authors.forename: Marina Gonçalves
authors.initials: MG
authors.lastname: Diniz
authors.forename: Erica Rievrs
authors.initials: ER
authors.lastname: Borges
authors.forename: Flavio Juliano
authors.initials: FJ
authors.lastname: Pimenta
authors.forename: Ana Carolina
authors.initials: AC
authors.lastname: De Mesquita Netto
authors.forename: Luiz
authors.initials: L
authors.lastname: De Marco
authors.forename: Ricardo Santiago
authors.initials: RS
authors.lastname: Gomez
authors.forename: Carolina Cavaliéri
authors.initials: CC
authors.lastname: Gomes
mesh_terms: D000293:Adolescent; D000328:Adult; D001859:Bone Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D002648:Child; D005260:Female; D018214:Fibroma, Ossifying / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D005354:Fibrosarcoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D016147:Genes, Tumor Suppressor; D006801:Humans; D007573:Jaw Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D010088:Oxidoreductases / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D025521:Tumor Suppressor Proteins / Q000235:genetics* / Q000378:metabolism; D000074584:WW Domain-Containing Oxidoreductase; D055815:Young Adult
chemical_list: D012333:RNA, Messenger; D025521:Tumor Suppressor Proteins; D010088:Oxidoreductases; D000074584:WW Domain-Containing Oxidoreductase; C486071:WWOX protein, human