title: The application of the RT-PCR method for the staging of the prostate cancer progression.
abstract: Molecular biology seems to bring more convincing markers for the detection of prostate cancer as well as the development of metastases than immunohistochemistry. The main goal of present work was to detect the expression of prostate specific antigen (PSA) and prostate-specific membrane antigen (PSM) genes in the micrometastases by the RT-PCR to assess the progression of prostate cancer. We analyzed 50 patients: 28 patients with clinically localized or locally advanced prostate cancer who underwent radical prostatectomy, 7 patients with clinically proven metastases, 8 patients with benign prostatic hyperplasia, and 7 healthy young men. The results of RT-PCR in the first group of 28 patients varied, however, they were in good correlation with the health status of the patients. Positive results of PSA and notably for PSM were good predictors of beginning metastasing process. Seven patients with metastatic disease had positive RT-PCR results both for PSA and PSM. All of the patients with benign prostatic hyperplasia and healthy young men had negative RT-PCR results for PSA and PSM. The study showed that positive RT-PCR results for PSA and especially for PSM correlated well with the progression of the disease and negative results reflected good health status of the patients.
authors.affiliation: Department of Pathology and Forensic Medicine, Health Care Surveillance Authority, Antolska 11, 851 07 Bratislava, Slovakia. anezka.zummerova@gmail.com
authors.forename: Anezka
authors.initials: A
authors.lastname: Zummerova
authors.forename: Peter
authors.initials: P
authors.lastname: Labas
authors.forename: Daniel
authors.initials: D
authors.lastname: Bohmer
authors.forename: Milan
authors.initials: M
authors.lastname: Blasko
authors.forename: Stefan
authors.initials: S
authors.lastname: Polak
authors.forename: Lubos
authors.initials: L
authors.lastname: Danisovic
authors.forename: Vanda
authors.initials: V
authors.lastname: Repiska
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000954:Antigens, Surface / Q000235:genetics; D018450:Disease Progression; D015972:Gene Expression Regulation, Neoplastic; D043425:Glutamate Carboxypeptidase II / Q000235:genetics; D006801:Humans; D008297:Male; D008875:Middle Aged; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging / Q000379:methods*; D017430:Prostate-Specific Antigen / Q000235:genetics; D011468:Prostatectomy; D011471:Prostatic Neoplasms / Q000097:blood / Q000235:genetics* / Q000473:pathology* / Q000601:surgery; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*
chemical_list: D000954:Antigens, Surface; C473848:FOLH1 protein, human; D043425:Glutamate Carboxypeptidase II; D017430:Prostate-Specific Antigen