title: [Role of NO signal pathway in the inhibitory of 17beta-estradiol on the production of endothelin-1 in vascular smooth muscle cells].
abstract: OBJECTIVE
  To investigate the mechanisms of 17beta-estradiol on the production of endothelin-1 in vascular smooth muscle cells.
  
  
  METHODS
  After incubation VSMC with various concentrations of 17beta-estradiol (10(-9) - 10(-7) mol/L) or plus L-NAME(10(- 6) mol/L) for different times, the concentration of endothelin-1 was measured. At the same time, the activity of endothelin converting enzyme-1 was analyzed, and the expression of preproET-1mRNA was measured by RT-PCR.
  
  
  RESULTS
  In basal conditions, 17beta-estradiol could inhibit the production of endothelin-1 in VSMC, and the action of 17beta-estradiol had nothing to do with the activity of endothelin converting enzyme-1. L-NAME inhibited the effect of 17-estradiol on the production of endothelin-1 in VSMC. RT-PCR results showed that 17-estradiol inhibited the preproET-1 mRNA expression, and whereas L-NAME reversed this action of 17beta-estradiol.
  
  
  CONCLUSIONS
  In basal conditions, 17beta-estradiol decreases the preproET-1 mRNA expression through NO-pathway to inhibit the production of endothelin-1 in cultured VSMC.
authors.affiliation: Department of Physiology, Zhongshan Medical College, Guangzhou 510080, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Tan
authors.forename: Ting-Huai
authors.initials: TH
authors.lastname: Wang
authors.forename: Yu
authors.initials: Y
authors.lastname: Chen
authors.forename: Giu-Ping
authors.initials: GP
authors.lastname: Lin
authors.forename: Jing-Yun
authors.initials: JY
authors.lastname: Pan
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D019332:Endothelin-1 / Q000096:biosynthesis*; D004958:Estradiol / Q000494:pharmacology*; D009131:Muscle, Smooth, Vascular / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D032389:Myocytes, Smooth Muscle / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D019332:Endothelin-1; D004958:Estradiol