title: Inhibitory effects of two major isoflavonoids in Radix Astragali on high glucose-induced mesangial cells proliferation and AGEs-induced endothelial cells apoptosis.
abstract: Radix Astragali, the dried roots of Astragalus membranaceus var. mongholicus, is well known to have a protective effect on diabetic nephropathy. However, the effects of isoflavonoids in Radix Astragali on glomerular cells, which play a key role in the development of diabetic vascular complications, remain largely unknown. Thus, the purpose of this study was to investigate in vitro the effect of calycosin and calycosin-7-O-β-D-glucoside, two major isoflavonoids in Radix Astragali, on high glucose-induced rat mesangial cells proliferation and AGEs-induced human glomerular endothelial cell apoptosis. The results indicated that both calycosin and calycosin-7-O-β-D-glucoside (10-100 µM) could inhibit high glucose-induced mesangial cell early proliferation. Additionally, AGEs-mediated cell apoptosis was also attenuated by treatment of glomerular endothelial cells with either calycosin or calycosin-7-O-β-D-glucoside (1-100 µM). Therefore, the results obtained in this study suggest that both calycosin and calycosin-7-O-β-D-glucoside have a significant therapeutic potential to modulate the development and/or progression of diabetic nephropathy.
authors.affiliation: Faculty of Chinese Medicine, Macau University of Science and Technology, Macao, PR China.
authors.forename: Dan
authors.initials: D
authors.lastname: Tang
authors.forename: Bao
authors.initials: B
authors.lastname: He
authors.forename: Zhao-Guang
authors.initials: ZG
authors.lastname: Zheng
authors.forename: Ru-Shang
authors.initials: RS
authors.lastname: Wang
authors.forename: Fei
authors.initials: F
authors.lastname: Gu
authors.forename: Ting-Ting
authors.initials: TT
authors.lastname: Duan
authors.forename: Hui-Quan
authors.initials: HQ
authors.lastname: Cheng
authors.forename: Quan
authors.initials: Q
authors.lastname: Zhu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D027883:Astragalus Plant / Q000737:chemistry; D027885:Astragalus propinquus / Q000737:chemistry; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000494:pharmacology; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D005947:Glucose / Q000494:pharmacology*; D005960:Glucosides / Q000494:pharmacology*; D017127:Glycation End Products, Advanced / Q000378:metabolism*; D006801:Humans; D007529:Isoflavones / Q000494:pharmacology*; D050527:Mesangial Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D018517:Plant Roots / Q000737:chemistry; D051381:Rats
chemical_list: D004365:Drugs, Chinese Herbal; D005960:Glucosides; D017127:Glycation End Products, Advanced; D007529:Isoflavones; C526426:calycosin-7-O-beta-D-glucoside; C121707:7,3'-dihydroxy-4'-methoxyisoflavone; C027492:Huang Qi; D005947:Glucose