title: PEGylated degradable composite nanoparticles based on mixtures of PEG-b-poly(γ-benzyl L-glutamate) and poly(γ-benzyl L-glutamate).
abstract: In the present work, the possibility to obtain PEGylated nanoparticles from two PBLG derivatives, PEG-b-poly(γ-benzyl L-glutamate), PBLG-PEG-60, and poly(γ-benzyl L-glutamate), PBLG-Bnz-50, by nanoprecipitation has been investigated. Particles were prepared not only from one polymer (PBLG-PEG-60 or PBLG-Bnz-50), but also from mixtures of two PBLG derivatives, PBLG-PEG-60 and PBLG-Bnz-50, in different ratios (90/10, 77/23, and 40/60 wt %). Because of the presence of PEG chains, hydrophilic particles were obtained, which was confirmed by ζ potential measurements (ζ from -13 mV and -21 mV) and by isothermal titration microcalorimetry (ITC). This last technique has shown no heat exchange when BSA was added to PEGylated nanoparticles. Further, complement activation has been evaluated by crossed immuno-electrophoresis demonstrating that the introduction of 77 wt % of PEGylated PBLG chains in the particles was enough to ensure a low complement activation activity. This effect was strongly correlated to the ζ potential of the particles, which decreased with an increase of PEG chains content. Interestingly, such properties are of interest for the preparation of degradable stealth nanocarriers. Moreover, it is suggested that the introduction of a reasonable amount (up to 20 wt %) of a second copolymer in the particle composition can be possible without modifying their stealth character. Moreover, the presence of this second copolymer would help to introduce a second functionality to the particles.
authors.affiliation: UMR CNRS 8612, Laboratoire de Pharmacie et Biopharmacie, Faculté de Pharmacie, Université Paris-Sud, Châtenay-Malabry, France.
authors.forename: Ma Elisa
authors.initials: ME
authors.lastname: Martínez-Barbosa
authors.forename: Sandrine
authors.initials: S
authors.lastname: Cammas-Marion
authors.forename: Laurent
authors.initials: L
authors.lastname: Bouteiller
authors.forename: Christine
authors.initials: C
authors.lastname: Vauthier
authors.forename: Gilles
authors.initials: G
authors.lastname: Ponchel
mesh_terms: D000906:Antibodies / Q000737:chemistry / Q000276:immunology; D000937:Antigen-Antibody Reactions; D002151:Calorimetry; D003176:Complement C3 / Q000737:chemistry / Q000276:immunology; D003179:Complement C3b / Q000737:chemistry / Q000276:immunology; D004587:Electrophoresis, Agar Gel; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D011092:Polyethylene Glycols / Q000138:chemical synthesis / Q000737:chemistry*; D011099:Polyglutamic Acid / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry / Q000276:immunology; D013499:Surface Properties
chemical_list: D000906:Antibodies; D003176:Complement C3; C003172:poly-gamma-benzyl-L-glutamate; D011099:Polyglutamic Acid; D011092:Polyethylene Glycols; D003179:Complement C3b