title: Direct electron transfer from glucose oxidase immobilized on polyphenanthroline-modified glassy carbon electrode.
abstract: This study reports direct electron transfer (DET) from immobilized glucose oxidase (GOx) via grafted and electropolymerized 1,10-phenanthroline monohydrate (PMH). The layer of poly-1,10-phenanthroline (PPMH) was gained via electrochemical deposition, which was used to create the PPMH-modified GC-electrode (PPMH/GC-electrode). Further, the GOx was immobilized on the PPMH/GC-electrode. The effect of surface-modification by the PPMH on the electron-transfer between enzyme and electrode-surface and some other electrochemical/analytical-parameters of newly designed enzymatic-electrode were evaluated. The PPMH/GC-electrode showed superior DET to/from flavine adenine dinucleotide cofactor of GOx, while some redox-compounds including ferrocene and K(3)[Fe(CN)(6)] were completely electrochemically inactive on the PPMH/GC-electrode. It was also found that the resulting GOx/PPMH/GC-electrode functioned as a "direct response type" glucose-biosensor. The biosensor showed excellent selectivity towards glucose and demonstrated good operational-stability. According to our best knowledge, this study is the first scientific report on electrochemical-polymerization of PMH on the GC-electrode in non-aqueous media followed by its application in the design of glucose-biosensor.
authors.affiliation: NanoTechnas-Center of Nanotechnology and Materials Science, Vilnius University, Faculty of Chemistry, Naugarduko 24, Vilnius, Lithuania.
authors.forename: Yasemin
authors.initials: Y
authors.lastname: Oztekin
authors.forename: Almira
authors.initials: A
authors.lastname: Ramanaviciene
authors.forename: Zafer
authors.initials: Z
authors.lastname: Yazicigil
authors.forename: Ali Osman
authors.initials: AO
authors.lastname: Solak
authors.forename: Arunas
authors.initials: A
authors.lastname: Ramanavicius
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D002244:Carbon / Q000737:chemistry*; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D003217:Conductometry / Q000295:instrumentation*; D004566:Electrodes; D004579:Electron Transport; D004800:Enzymes, Immobilized / Q000737:chemistry; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005898:Glass; D005947:Glucose / Q000032:analysis* / Q000737:chemistry; D005949:Glucose Oxidase / Q000737:chemistry*; D010618:Phenanthrolines / Q000737:chemistry*
chemical_list: D020099:Coated Materials, Biocompatible; D004800:Enzymes, Immobilized; D010618:Phenanthrolines; D002244:Carbon; D005949:Glucose Oxidase; D005947:Glucose