title: [Influence of vasoactive intestinal peptide on expression of pulmonary surfactant associated protein A in lung explants].
abstract: OBJECTIVE
  To study the influence of VIP on the expression of SP-A and its intracellular signal transduction pathway.
  
  
  METHODS
  The influence of VIP on the expression of SP-A was studied by immunohistochemistry and RT-PCR. The intracellular signal transduction pathway was further investigated by using receptor antagonist, protein kinase inhibitor and antisense oligonucleotides.
  
  
  RESULTS
  (1) VIP(10(-8) mol/L) enhanced SP-A protein expression in alveolar type II cells (ATII) and increased the content of SP-A mRNA in lung tissue. (2) VIP receptor antagonist [D-P-C1-Phe (6)-Leu (17)]-VIP (10(-6) mol/L) could suppress the VIP-induced expression of SP-A protein and SP-A mRNA. (3) c-fos antisense oligonucleotides (9 x 10(-6) mol/L) could inhibit the VIP-induced expression of SP-A protein and SP-A mRNA. (4) Protein kinase C(PKC) inhibitor H7 (10(-5) mol/L) could also depress the V1P-induced SP-A protein and SP-A mRNA.
  
  
  CONCLUSIONS
  VIP can up-regulate the expression of SP-A through its receptor. PKC and c-fos protein play important roles in the intracellular signal transduction pathway through which VIP induces the expression of SP-A.
authors.affiliation: Department of Physiology, Xiang Ya School of Medicine, Central South University, Changsha 410078, China.
authors.forename: Lian
authors.initials: L
authors.lastname: Li
authors.forename: Zi-qiang
authors.initials: ZQ
authors.lastname: Luo
authors.forename: Gan-qiou
authors.initials: GQ
authors.lastname: Wu
authors.forename: Xiu-hong
authors.initials: XH
authors.lastname: Sun
mesh_terms: D000818:Animals; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism*; D066298:In Vitro Techniques; D011493:Protein Kinase C / Q000378:metabolism; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism; D011650:Pulmonary Alveoli / Q000166:cytology; D037662:Pulmonary Surfactant-Associated Protein A / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology*
chemical_list: D016760:Proto-Oncogene Proteins c-fos; D037662:Pulmonary Surfactant-Associated Protein A; D014660:Vasoactive Intestinal Peptide; D011493:Protein Kinase C