title: [Protective effect of Naoyikang on the Alzheimer's disease model mice induced by D-galactose and NaNO2].
abstract: OBJECTIVE
  To investigate the mechanisms of Naoyikang (Traditional Chinese Medicine) on the Alzheimer's Disease (AD) model mice induced by D-galactose (D-gal) and NaNO2.
  
  
  METHODS
  The mouse model was established by intraperitoneal injection of D-gal and NaNO2. The capacity of learning and memory was tested on mice with electrical maze; the content of nitric oxide (NO) and the activity of monoamine oxidase-B (MAO-B), glutathione peroxidase (GSH-PX), Na(+) -K(+) -ATP enzyme and Ca(2+) -ATP enzyme in cerebral cortex and hippocampus were assayed by biochemical methods; expression of Bax and Bcl-2 mRNA was detested by RT-PCR.
  
  
  RESULTS
  Naoyikang could ameliorate the capacity of learning and memory of AD model mice and reduce MAO-B activity in the brain tissue and activate the activity of Na(+) -K(+) -ATP enzyme and Ca(2+) -ATP enzyme in the brain tissue and decrease the expression of Bax mRNA, but increase the expression of Bcl-2 mRNA in the model brain tissue.
  
  
  CONCLUSIONS
  Naoyikang could protect AD model mice induced by D-gal and NaNO2. It could modify the metabolism of monoamine neurotransmitter in brain through reducing MAO-B activity and protect neurons by activating the activity of Na(+) -K(+) -ATP enzyme and Ca(2+) -ATP enzyme and decrease Bax expression and increase Bcl-2 expression in the model brain tissue.
authors.affiliation: Department of Pathophysiology, School of Medical Sciences, Nantong University, Nantong 226001, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhu
authors.forename: Ai-Ling
authors.initials: AL
authors.lastname: Zhou
authors.forename: Jia-Hui
authors.initials: JH
authors.lastname: Mao
authors.forename: Ya-E
authors.initials: YE
authors.lastname: Hu
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: Shi
mesh_terms: D000544:Alzheimer Disease / Q000139:chemically induced / Q000188:drug therapy*; D000818:Animals; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D005260:Female; D005690:Galactose; D008297:Male; D018782:Maze Learning; D051379:Mice; D008813:Mice, Inbred ICR; D018696:Neuroprotective Agents / Q000627:therapeutic use*; D008517:Phytotherapy; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011897:Random Allocation; D012977:Sodium Nitrite; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000235:genetics / Q000378:metabolism
chemical_list: D004365:Drugs, Chinese Herbal; D018696:Neuroprotective Agents; D012333:RNA, Messenger; D051028:bcl-2-Associated X Protein; D000254:Sodium-Potassium-Exchanging ATPase; D012977:Sodium Nitrite; D005690:Galactose