title: Synergistic anticancer effects achieved by co-delivery of TRAIL and paclitaxel using cationic polymeric micelles.
abstract: Cationic micellar nanoparticles self-assembled from a biodegradable amphiphilic copolymer have been used to deliver human TRAIL and paclitaxel simultaneously. Polyplexes formed between paclitaxel-loaded nanoparticles and TRAIL are stable with a size of ≈180 nm and a zeta potential at ≈75 mV. Anticancer effects and apoptotic pathway mechanisms of this drug-and-protein co-delivery system are investigated in various human breast cancer cell lines with different TRAIL sensitivity. The co-delivery nanoparticulate system induces synergistic anti-cancer activities with limited toxicity in non-cancerous cells. An advantage of this co-delivery is a significantly higher anti-cancer effect as compared to free drug and protein formulations.
authors.affiliation: Institute of Bioengineering and Nanotechnology, 31 Biopolis Way, The Nanos, Singapore 138669, Singapore.
authors.forename: Ashlynn L Z
authors.initials: AL
authors.lastname: Lee
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.forename: Shazib
authors.initials: S
authors.lastname: Pervaiz
authors.forename: Weimin
authors.initials: W
authors.lastname: Fan
authors.forename: Yi Yan
authors.initials: YY
authors.lastname: Yang
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D020169:Caspases / Q000378:metabolism; D002412:Cations; D002453:Cell Cycle / Q000187:drug effects; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004357:Drug Synergism; D024202:Electrophoretic Mobility Shift Assay; D006801:Humans; D008823:Micelles; D053758:Nanoparticles / Q000737:chemistry; D017239:Paclitaxel / Q000737:chemistry / Q000494:pharmacology*; D010316:Particle Size; D011108:Polymers / Q000737:chemistry / Q000494:pharmacology*; D055672:Static Electricity; D053221:TNF-Related Apoptosis-Inducing Ligand / Q000494:pharmacology*; D013997:Time Factors; D014410:Tumor Stem Cell Assay
chemical_list: D000970:Antineoplastic Agents; D002412:Cations; D008823:Micelles; D011108:Polymers; D053221:TNF-Related Apoptosis-Inducing Ligand; D020169:Caspases; D017239:Paclitaxel