title: Cryo-EM structure of the E. coli translating ribosome in complex with SRP and its receptor.
abstract: We report the 'early' conformation of the Escherichia coli signal recognition particle (SRP) and its receptor FtsY bound to the translating ribosome, as determined by cryo-EM. FtsY binds to the tetraloop of the SRP RNA, whereas the NG domains of the SRP protein and FtsY interact weakly in this conformation. Our results suggest that optimal positioning of the SRP RNA tetraloop and the Ffh NG domain leads to FtsY recruitment.
authors.affiliation: European Molecular Biology Laboratory, Grenoble Outstation, Grenoble, France.
authors.forename: Leandro F
authors.initials: LF
authors.lastname: Estrozi
authors.forename: Daniel
authors.initials: D
authors.lastname: Boehringer
authors.forename: Shu-Ou
authors.initials: SO
authors.lastname: Shan
authors.forename: Nenad
authors.initials: N
authors.lastname: Ban
authors.forename: Christiane
authors.initials: C
authors.lastname: Schaffitzel
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D020285:Cryoelectron Microscopy; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000378:metabolism; D008958:Models, Molecular; D014176:Protein Biosynthesis; D017434:Protein Structure, Tertiary; D018160:Receptors, Cytoplasmic and Nuclear / Q000737:chemistry* / Q000378:metabolism; D018000:Receptors, Peptide / Q000737:chemistry*; D012270:Ribosomes / Q000737:chemistry / Q000648:ultrastructure*; D018271:Signal Recognition Particle / Q000737:chemistry* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; C087406:FtsY protein, Bacteria; D018160:Receptors, Cytoplasmic and Nuclear; D018000:Receptors, Peptide; D018271:Signal Recognition Particle; C036318:signal peptide receptor