title: The tear fluid mucin 5AC change of primary angle-closure glaucoma patients after short-term medications and phacotrabeculectomy.
abstract: OBJECTIVE
  This paper proposed to evaluate the tear fluid mucin 5AC (MUC5AC) change in Chinese primary angle-closure glaucoma and cataract patients after short-term medications and phacotrabeculectomy.
  
  
  METHODS
  Twenty-five eyes of 25 consecutive Chinese patients with coexisting visually significant cataract and angle-closure glaucoma and 40 eyes of 40 volunteers enrolled in this study were investigated. Tear fluid from normal subjects and patients (1 day pre-operatively, 1 month, 3 months, and 6 months post-operatively, respectively) were collected. The MUC5AC protein levels in the tear fluid were determined by Enzyme-Linked ImmunoSorbent Assay (ELISA). The MUC5AC change after phacotrabeculectomy was evaluated.
  
  
  RESULTS
  The MUC5AC quantity of the patients after short-term medications was 16.95±12.86 ng/ml, compared with 32.39±18.44 ng/ml MUC5AC quantity of the controls. There was a significant difference between the two groups (p<0.05). The MUC5AC of the patients decreased significantly to 6.91±7.11 ng/ml at 1 month after surgery (p<0.05). At 3 months after surgery, the MUC5AC recovered to 15.53±12.63 ng/ml, and had no significant difference with the pre-operative level (p=0.26). At 6 months after surgery, the MUC5AC was 18.94±14.64 ng/ml and had no significant difference with before-surgery levels (p=0.14).
  
  
  CONCLUSIONS
  Both phacotrabeculectomy and short-term anti-glaucoma medications can decrease the MUC5AC in the tear fluid of primary angle-closure glaucoma patients. At 3 months after phacotrabeculectomy, the MUC5AC could recover to the pre-operative level. More attention should be paid to the tear film stability and ocular surface physiology in these patients.
authors.affiliation: Tianjin Medical University Eye Center, Tianjin, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Huilin
authors.initials: H
authors.lastname: Li
authors.forename: Duanyang
authors.initials: D
authors.lastname: Lu
authors.forename: Juan
authors.initials: J
authors.lastname: Liang
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Xing
authors.forename: Aihua
authors.initials: A
authors.lastname: Liu
authors.forename: Shaozhen
authors.initials: S
authors.lastname: Zhao
authors.forename: Xiaorong
authors.initials: X
authors.lastname: Li
authors.forename: Jian
authors.initials: J
authors.lastname: Ji
mesh_terms: D000368:Aged; D016022:Case-Control Studies; D005260:Female; D015812:Glaucoma, Angle-Closure / Q000188:drug therapy* / Q000378:metabolism / Q000601:surgery*; D006801:Humans; D008297:Male; D055271:Mucin 5AC / Q000378:metabolism*; D013666:Tears / Q000378:metabolism*; D013997:Time Factors; D014130:Trabeculectomy
chemical_list: D055271:Mucin 5AC