title: In vivo comparison of the effects of rhBMP-2 and rhBMP-4 in osteochondral tissue regeneration.
abstract: The aim of this work is to investigate the use of bone morphogenetic proteins (rhBMP-2, rhBMP-4) alone or in combination with cells delivered in a calcium alginate gel for the treatment of osteochondral defects. For this purpose, alginate gels were prepared mixing a 2% sodium alginate solution and a 200 mM calcium chloride solution (1:1). Osteochondral defects were created (4 mm wide, 5 mm deep) in the internal femoral condyle of rabbit knee and gels were directly formed into the defects. 3 months after surgery samples were harvested, gross morphology was documented and histological appearance was evaluated. The performed histological observations revealed subchondral bone regeneration in rhBMP-2 samples and moderate hyaline cartilage regeneration in rhBMP-4 samples. Thus, results indicate that alginate gel may serve as an appropriate delivery vehicle for rhBMP-2, rhBMP-4 and stromal cells. With this carrier material, differential behaviour between the evaluated proteins was observed. rhBMP-2 shows better restoration of subchondral bone in contrast to the superior efficiency of rhBMP-4 for hyaline cartilage repair.
authors.affiliation: Hospital Clínico San Carlos, 28040 Madrid, Spain.
authors.forename: Yaiza
authors.initials: Y
authors.lastname: Lópiz-Morales
authors.forename: Ander
authors.initials: A
authors.lastname: Abarrategi
authors.forename: Viviana
authors.initials: V
authors.lastname: Ramos
authors.forename: Carolina
authors.initials: C
authors.lastname: Moreno-Vicente
authors.forename: Luis
authors.initials: L
authors.lastname: López-Durán
authors.forename: José Luis
authors.initials: JL
authors.lastname: López-Lacomba
authors.forename: Fernando
authors.initials: F
authors.lastname: Marco
mesh_terms: D000464:Alginates; D000818:Animals; D055396:Bone Morphogenetic Protein 2; D055415:Bone Morphogenetic Protein 4 / Q000096:biosynthesis / Q000494:pharmacology*; D019485:Bone Morphogenetic Proteins / Q000494:pharmacology*; D001861:Bone Regeneration / Q000187:drug effects*; D004337:Drug Carriers; D005269:Femur / Q000187:drug effects / Q000473:pathology; D005782:Gels; D020723:Glucuronic Acid; D048091:Guided Tissue Regeneration; D006603:Hexuronic Acids; D006801:Humans; D051379:Mice; D019736:Prostheses and Implants; D011817:Rabbits; D011994:Recombinant Proteins / Q000494:pharmacology*; D016212:Transforming Growth Factor beta / Q000494:pharmacology*
chemical_list: D000464:Alginates; C525809:BMP4 protein, human; D055396:Bone Morphogenetic Protein 2; D055415:Bone Morphogenetic Protein 4; D019485:Bone Morphogenetic Proteins; D004337:Drug Carriers; D005782:Gels; D006603:Hexuronic Acids; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; C499469:recombinant human bone morphogenetic protein-2; D020723:Glucuronic Acid