title: [The protective effect of melatonin on auditory cortex toxicity induced by cis-platinum].
abstract: OBJECTIVE
  To investigate the toxic response in auditory cortex of guinea pigs caused by cis-platinum (DDP), and the protective role of melatonin in this effect.
  
  
  METHODS
  Cis-platinum and melatonin were injected peritoneally. LDH, MDA, NO in the auditory cortex were detected by spectrophotometeR.
  
  
  RESULTS
  The body weight of the guinea pigs was diminished by peritoneal injection of Cis-platinum for 7 days (P < 0.01). Peritoneal injection of Cis-platinum induced the increased leakage of LDH (P < 0.05 vs injection of normal saline). This effect was reduced by injection of MT (P < 0.05). The content of MDA in the auditory cortex was also increased because of injection of Cis-platinumv for 7 days (P < 0.01) and MT reduced this effect (P < 0.05). The change of NO in the auditory cortex was not statistically significant after injection of Cis-platinum or Cis-platinum with MT.
  
  
  CONCLUSIONS
  Peritoneal injection of Cis-platinum could destroy neurons in the auditory cortex. This effect could be reduced by melatonin by an anti-free radials mechanism.
authors.affiliation: Department of Basic Medical Sciences, College of Medicine, Zhejiang University, Hangzhou 310058, China.
authors.forename: Ming-De
authors.initials: MD
authors.lastname: Huang
authors.forename: Xia
authors.initials: X
authors.lastname: Sun
authors.forename: Xi
authors.initials: X
authors.lastname: Cao
authors.forename: Qiong-Yao
authors.initials: QY
authors.lastname: Hu
authors.forename: Meng-Hui
authors.initials: MH
authors.lastname: Zhao
authors.forename: Yan-Qin
authors.initials: YQ
authors.lastname: Yu
mesh_terms: D000818:Animals; D001303:Auditory Cortex / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D002945:Cisplatin / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D005260:Female; D016166:Free Radical Scavengers / Q000494:pharmacology*; D006168:Guinea Pigs; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008550:Melatonin / Q000494:pharmacology*; D009474:Neurons / Q000187:drug effects / Q000473:pathology; D011897:Random Allocation
chemical_list: D016166:Free Radical Scavengers; D008315:Malondialdehyde; D008550:Melatonin; D002945:Cisplatin