title: Single-cell adhesion tests against functionalized microspheres arrayed on AFM cantilevers confirm heterophilic E- and N-cadherin binding.
abstract: We assess the cross-reactivity of both cellular as well as recombinant E- and N-cadherins using functionalized bead arrays assembled on atomic-force-microscope cantilevers. This new approach builds upon and enhances the utility of a recently developed force probe that integrates a custom-built, horizontal atomic force microscope with micropipette manipulation. It enables us to test multiple biomolecular interactions of the same cell in a swift sequential or cyclic manner and thus to resolve subtle differences between individual interactions that otherwise would be obscured by cell-cell baseline variability. For each cell, we contrast heterophilic E:N-cadherin binding with the respective homophilic bonds and with a suitable control. Clarifying previous literature reports, we establish that specific bonds between E- and N-cadherins form readily, albeit less frequently than homophilic bonds of either cadherin. We support this assessment with a rough estimate of the ratio of on-rate constants of E/N-cadherin binding.
authors.affiliation: Department of Biomedical Engineering, University of California at Davis, Davis, California, USA.
authors.forename: Chawin
authors.initials: C
authors.lastname: Ounkomol
authors.forename: Soichiro
authors.initials: S
authors.lastname: Yamada
authors.forename: Volkmar
authors.initials: V
authors.lastname: Heinrich
mesh_terms: D000818:Animals; D015820:Cadherins / Q000378:metabolism*; D002448:Cell Adhesion; D006801:Humans; D007739:L Cells; D051379:Mice; D018625:Microscopy, Atomic Force / Q000295:instrumentation* / Q000379:methods*; D008863:Microspheres; D011485:Protein Binding
chemical_list: D015820:Cadherins