title: Multiresidue analysis of multiclass pesticides in lavandin essential oil by LC/MS/MS using the scheduled selected reaction monitoring mode.
abstract: In this paper we describe the development of the first multiclass pesticide residue method applied to essential oils. A total of 70 pesticides covering a wide range of polarity and currently used on essential oil crops have been included in the method. The procedure consists of a 10-fold dilution of lavandin essential oil followed by a direct injection analysis by liquid chromatography/tandem mass spectrometry. The system used is an API 4000 QTrap equipped with an electrospray ionization interface and operating in scheduled selected reaction monitoring acquisition mode. Matrix effects were evaluated by comparing the slopes of matrix-matched and solvent-based calibration curves. Weak signal suppression or enhancement (<20%) was observed for most of the compounds. Method sensitivity was determined statistically by the injection of five matrix-matched calibration curves with the distribution's normality and the variance's homogeneity checked before establishment of a suitable regression model. Limits of detection (LODs) and quantification (LOQs) were then determined using the blank standard's deviation and the slope of the mean curve. The analytical method has been validated for 67 of the 70 pesticides and meets the following LOQs: ≤1 μg/L for 9 pesticides, ≤5 μg/L for 44, ≤10 μg/L for 9, and ≤20 μg/L for 5.
authors.affiliation: Institut des Sciences et Technologies Moléculaires d'Angers (MOLTECH), CNRS UMR 6200, and Laboratoire d'Etudes Environmentales des Systèmes Anthropisés (LEESA), Université d'Angers, 2 boulevard Lavoisier, 49045 Angers Cedex 01, France.
authors.forename: Yoann
authors.initials: Y
authors.lastname: Fillâtre
authors.forename: David
authors.initials: D
authors.lastname: Rondeau
authors.forename: Brice
authors.initials: B
authors.lastname: Bonnet
authors.forename: Antoine
authors.initials: A
authors.lastname: Daguin
authors.forename: Alain
authors.initials: A
authors.lastname: Jadas-Hécart
authors.forename: Pierre-Yves
authors.initials: PY
authors.lastname: Communal
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D007267:Injections; D027523:Lavandula / Q000737:chemistry*; D057230:Limit of Detection; D009822:Oils, Volatile / Q000737:chemistry*; D010573:Pesticide Residues / Q000032:analysis* / Q000145:classification; D015203:Reproducibility of Results; D012997:Solvents / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D009822:Oils, Volatile; D010573:Pesticide Residues; D012997:Solvents