title: Identification of a highly immunoreactive epitope of Brugia malayi TPx recognized by the endemic sera.
abstract: Filarial thiordoxin peroxidase is a major antioxidant that plays a crucial role in parasite survival. Although Brugia malayi TPx has been shown to be a potential vaccine candidate, it shares 63% homology with its mammalian counterpart, limiting its use as a vaccine or drug target. In silico analysis of TPx sequence revealed a linear B epitope in the host's nonhomologous region. The peptide sequence (TPx peptide(27-48)) was synthesized, and its reactivity with clinical sera from an endemic region was analyzed. The peptide showed significantly high reactivity (P < 0.05) against the sera of putatively immune individuals compared to the nonendemic control sera. It also showed high reactivity against the sera of patients with chronic pathology and patent infection. The high reactivity of the peptide with endemic immune sera equivalent to that of whole protein shows that it forms a dominant B epitope of TPx protein and thus could be utilized for incorporation into a multiepitope vaccine construct for filariasis.
authors.affiliation: Centre for Biotechnology, Anna University, Chennai 600025, India.
authors.forename: Jayaprakasam
authors.initials: J
authors.lastname: Madhumathi
authors.forename: Prabhu Rajaiah
authors.initials: PR
authors.lastname: Prince
authors.forename: Subash Chellam
authors.initials: SC
authors.lastname: Gayatri
authors.forename: Ramanathan
authors.initials: R
authors.lastname: Aparnaa
authors.forename: Perumal
authors.initials: P
authors.lastname: Kaliraj
mesh_terms: D000818:Animals; D017178:Brugia malayi / Q000201:enzymology* / Q000276:immunology; D004605:Elephantiasis, Filarial / Q000097:blood / Q000276:immunology* / Q000469:parasitology; D019353:Endemic Diseases; D018985:Epitopes, B-Lymphocyte / Q000276:immunology; D006801:Humans; D007106:Immune Sera / Q000276:immunology*; D010455:Peptides / Q000737:chemistry / Q000276:immunology; D054464:Peroxiredoxins / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification; D014612:Vaccines / Q000276:immunology
chemical_list: D018985:Epitopes, B-Lymphocyte; D007106:Immune Sera; D010455:Peptides; D011994:Recombinant Proteins; D014612:Vaccines; D054464:Peroxiredoxins