title: A suggested model for potato MIVOISAP involving functions of central carbohydrate and amino acid metabolism, as well as actin cytoskeleton and endocytosis.
abstract: We have recently found that microbial species ranging from Gram-negative and Gram-positive bacteria to different fungi emit volatiles that strongly promote starch accumulation in leaves of both mono- and di-cotyledonous plants. Transcriptome and enzyme activity analyses of potato leaves exposed to volatiles emitted by Alternaria alternata revealed that starch over-accumulation was accompanied by enhanced 3-phosphoglycerate to Pi ratio, and changes in functions involved in both central carbohydrate and amino acid metabolism. Exposure to microbial volatiles also promoted changes in the expression of genes that code for enzymes involved in endocytic uptake and traffic of solutes. With the overall data we propose a metabolic model wherein important determinants of accumulation of exceptionally high levels of starch include (a) upregulation of ADPglucose-producing SuSy, starch synthase III and IV, proteins involved in the endocytic uptake and traffic of sucrose, (b) down-regulation of acid invertase, starch breakdown enzymes and proteins involved in internal amino acid provision, and (c) 3-phosphoglycerate-mediated allosteric activation of ADPglucose pyrophosphorylase.
authors.affiliation: Instituto de Agrobiotecnología (CSIC/UPNA/Gobierno de Navarra), Mutiloako etorbidea z/g, Nafarroa, Spain.
authors.forename: Ignacio
authors.initials: I
authors.lastname: Ezquer
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.forename: Miroslav
authors.initials: M
authors.lastname: Ovecka
authors.forename: Edurne
authors.initials: E
authors.lastname: Baroja-Fernández
authors.forename: Francisco José
authors.initials: FJ
authors.lastname: Muñoz
authors.forename: Manuel
authors.initials: M
authors.lastname: Montero
authors.forename: Jessica
authors.initials: J
authors.lastname: Díaz de Cerio
authors.forename: Maite
authors.initials: M
authors.lastname: Hidalgo
authors.forename: María Teresa
authors.initials: MT
authors.lastname: Sesma
authors.forename: Abdellatif
authors.initials: A
authors.lastname: Bahaji
authors.forename: Ed
authors.initials: E
authors.lastname: Etxeberria
authors.forename: Javier
authors.initials: J
authors.lastname: Pozueta-Romero
mesh_terms: D000199:Actins / Q000378:metabolism*; D000596:Amino Acids / Q000378:metabolism*; D050260:Carbohydrate Metabolism; D003599:Cytoskeleton / Q000378:metabolism*; D004705:Endocytosis; D008954:Models, Biological; D011198:Solanum tuberosum / Q000378:metabolism*
chemical_list: D000199:Actins; D000596:Amino Acids