title: [Experimental research on permeability injury of pulmonary microvascular endothelial cells monolayer induced by tumor necrosis factor].
abstract: OBJECTIVE
  To investigate the mechanism of tumor necrosis factor (TNF) induced permeability injury of rat pulmonary microvascular endothelial cells (RPMVEC) monolayer, the effect of TNF on permeability of RPMVEC monolayer was examined with microfilter and the effect of TNF on RPMVEC F-actin was observed with immunocytochemistry. The interfering action of formoterol, anisodamine and cholera toxin on permeability and F-actin changes induced by TNF was also observed.
  
  
  RESULTS
  (1) TNF induced significant increase in permeability of RPMVEC monolayer 30, 60 and 90 minutes after treatment with TNF. (2) F-actin in RPMVEC depolymerized 90 minutes after treatment with TNF. Permeability and F-actin did not change significantly when formoterol, anisodamine or cholera toxin was added separately.
  
  
  CONCLUSIONS
  TNF can induce permeability injury of RPMVEC monolayer, which is correlated with depolymerization of F-actin. Formoterol, anisodamine and cholera toxin can inhibit the permeability change induced by TNF which may due to their inhibition to the distribution change of F-actin.
authors.affiliation: Respiratory Institute of Xinqiao Hospital, Third Military Medical University, Chongqing 400037.
authors.forename: Z L
authors.initials: ZL
authors.lastname: Xiao
authors.forename: G Y
authors.initials: GY
authors.lastname: Sun
authors.forename: Q M
authors.initials: QM
authors.lastname: Xia
authors.forename: G S
authors.initials: GS
authors.lastname: Qian
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D002463:Cell Membrane Permeability / Q000187:drug effects*; D002478:Cells, Cultured; D002772:Cholera Toxin / Q000494:pharmacology; D042783:Endothelial Cells / Q000187:drug effects*; D004730:Endothelium, Vascular / Q000166:cytology* / Q000378:metabolism; D004983:Ethanolamines / Q000494:pharmacology; D000068759:Formoterol Fumarate; D008168:Lung / Q000098:blood supply; D051381:Rats; D017208:Rats, Wistar; D012991:Solanaceous Alkaloids / Q000494:pharmacology; D048069:Tumor Necrosis Factors / Q000494:pharmacology*
chemical_list: D000199:Actins; D004983:Ethanolamines; D012991:Solanaceous Alkaloids; D048069:Tumor Necrosis Factors; C003922:anisodamine; D002772:Cholera Toxin; D000068759:Formoterol Fumarate