title: Burkitt's lymphoma: maximising the use of fine needle aspirates by long-term preservation for diagnosis and research.
abstract: Fine needle aspirates from Burkitt's lymphoma and other tumours transferred directly into ThinPrep® PreservCyt® (Cytyc UK Ltd, Crawley, UK) buffered alcohol fixative retain their cellular and viral antigens and nucleic acids for many months at ambient temperatures. Despite the presence of blood and debris, cells dried onto slides from droplets and post-fixed in formalin, or sections of paraffin-embedded cell blocks from formalin post-fixed pellets, prove adequate for morphology, immunocytochemistry, in-situ hybridization and molecular biological analyses. Where there is lack of expertise in making thin smears or hospitals lack pathology laboratories and services, PreservCyt® provides an excellent medium for transport elsewhere for diagnosis and research.
authors.affiliation: Department of Histopathology, Imperial College London, Hammersmith Hospital, Du Cane Road, London W12 ONN, UK. s.vannoorden@imperial.ac.uk
authors.forename: Susan
authors.initials: S
authors.lastname: Van Noorden
authors.forename: Irvin A
authors.initials: IA
authors.lastname: Lampert
authors.forename: Shao-An
authors.initials: SA
authors.lastname: Xue
authors.forename: Dimitrios
authors.initials: D
authors.lastname: Lykidis
authors.forename: John A
authors.initials: JA
authors.lastname: Phillips
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Molyneux
authors.forename: Beverly E
authors.initials: BE
authors.lastname: Griffin
mesh_terms: D000293:Adolescent; D001707:Biopsy, Needle / Q000379:methods*; D002051:Burkitt Lymphoma / Q000453:epidemiology / Q000473:pathology*; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007223:Infant; D008295:Malawi / Q000453:epidemiology; D008297:Male; D016612:Paraffin Embedding; D012680:Sensitivity and Specificity; D012959:Socioeconomic Factors; D013048:Specimen Handling