title: [The influence of acetylcholine on the proliferation of cultured human pituitary tumour cells].
abstract: OBJECTIVE
  To study the influence of acetylcholine on the proliferation, DNA synthesis and cell cycle of cultured human pituitary tumour cells.
  
  
  METHODS
  MTT method, 3H-TdR incorporation and cell cycle analysis were used to examine the changes of proliferation and DNA synthesis of human pituitary tumour cells.
  
  
  RESULTS
  Ach at 10(-7) mol/L - 10(-5) mol/L could decrease the 3H-TdR incorporation and the MTT A value in a dose dependent manner (P < 0.01), and the ratio of G1 phase of pituitary tumour cells increased markedly (P < 0.01). The effect of acetylcholine on the proliferation of cultured human pituitary tumour cells could be inhibited by atropine.
  
  
  CONCLUSIONS
  Ach inhibited the proliferation, DNA synthesis and cell cycle of cultured human pituitary tumour cells, and the inhibitory effect was mediated by acetylcholine receptor.
authors.affiliation: Department of Physiology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: S M
authors.initials: SM
authors.lastname: Chi
authors.forename: Y L
authors.initials: YL
authors.lastname: Zhu
authors.forename: J W
authors.initials: JW
authors.lastname: Gu
authors.forename: L
authors.initials: L
authors.lastname: Du
authors.forename: F Z
authors.initials: FZ
authors.lastname: Wang
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology*; D000236:Adenoma / Q000473:pathology; D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D006801:Humans; D010911:Pituitary Neoplasms / Q000473:pathology; D014407:Tumor Cells, Cultured
chemical_list: D000109:Acetylcholine