title: [Effects of scorpion venom heat-resistant protein on kainic acid induced-damage of cultured primitive rat hippocampal neuropeptide Y-nergic neurons].
abstract: OBJECTIVE
  To investigate the effects of scorpion venom heat-resistant protein (SVHRP) on kainic acid induced-damage of cultured primitive rat hippocampal neuropeptide Y-nergic neurons.
  
  
  METHODS
  We observed morphological changes, celluar vigor, NPY-immunoreactivity and NPY mRNA expression by means of Thionine staining, MTT assay, immunocytochemistry and RT-PCR, respectively, on the primitively cultured Sprague-Dawley rat hippocampal neuron treated with KA and SVHRP for 24 h.
  
  
  RESULTS
  MTT assay and morphologic analysis showed that SVHRP markedly increased neuron survival-rate, and protected them from kA-induced damage. The expression of NPY-immunoreactivity and NPY mRNA in SVHRP group increased obviously compared with other groups.
  
  
  CONCLUSIONS
  SVHRP protected the primitively cultured hippocampal neurons from KA-induced neuroexcitotoxicity and promoted the expression of NPY.
authors.affiliation: Department of Physiology, Dalian Medical University, Dalian 116027, China.
authors.forename: Yan-Hui
authors.initials: YH
authors.lastname: Feng
authors.forename: De-Qin
authors.initials: DQ
authors.lastname: Yu
authors.forename: Yan
authors.initials: Y
authors.lastname: Peng
authors.forename: Sheng-Ming
authors.initials: SM
authors.lastname: Yin
authors.forename: Yi-Ping
authors.initials: YP
authors.lastname: Sun
authors.forename: Xue-Fei
authors.initials: XF
authors.lastname: Wu
authors.forename: Wan-Qin
authors.initials: WQ
authors.lastname: Zhang
authors.forename: Jie
authors.initials: J
authors.lastname: Zhao
mesh_terms: D000818:Animals; D016923:Cell Death; D002478:Cells, Cultured; D006624:Hippocampus / Q000166:cytology* / Q000378:metabolism; D007608:Kainic Acid / Q000494:pharmacology*; D008297:Male; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D009478:Neuropeptide Y / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012604:Scorpion Venoms / Q000494:pharmacology*
chemical_list: D009478:Neuropeptide Y; D012604:Scorpion Venoms; D007608:Kainic Acid