title: Glycol methacrylate embedding for improved morphological, morphometrical, and immunohistochemical investigations under light microscopy: testes as a model.
abstract: Glycol methacrylate (GMA), a water and ethanol miscible plastic resin, is a medium handy to use for light microscopy embedding that has a number of advantages than paraffin embedding. The GMA improves the histological, morphometrical, and immunohistochemical evaluations, mainly due to the accurate assessment of cytological details. This chapter focuses on our experience in the GMA processing and describes in detail the fixation, embedding, and staining methods that we have been using for testes evaluations.
authors.affiliation: Laboratory of Structural Biology and Reproduction, Department of Morphology, ICB, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil. chiarini@icb.ufmg.br
authors.forename: Hélio
authors.initials: H
authors.lastname: Chiarini-Garcia
authors.forename: Gleydes Gambogi
authors.initials: GG
authors.lastname: Parreira
authors.forename: Fernanda R C L
authors.initials: FR
authors.lastname: Almeida
mesh_terms: D000818:Animals; D007150:Immunohistochemistry / Q000379:methods*; D008297:Male; D008322:Mammals; D008689:Methacrylates / Q000737:chemistry; D008853:Microscopy / Q000379:methods*; D013737:Testis / Q000648:ultrastructure*; D016610:Tissue Embedding / Q000379:methods*; D016707:Tissue Fixation / Q000379:methods
chemical_list: D008689:Methacrylates; C005044:hydroxyethyl methacrylate