title: Effects of 530 nm green light on refractive status, melatonin, MT1 receptor, and melanopsin in the guinea pig.
abstract: OBJECTIVE
  To investigate (i) the effect of monochromatic light on inhibiting induction of light-induced melatonin and (ii) the roles of melanopsin and MT1 receptor in light-induced myopia in the guinea pig.
  
  
  METHODS
  Forty-eight guinea pigs were randomly distributed into three treatment groups: white-light (control), green-light (530 nm), and blue-light (480 nm) groups. Levels of pineal gland melatonin were measured twice daily--10:00 a.m. and 10:00 p.m.--10 days after initial light treatment. Thirty additional guinea pigs were also assigned to these groups and treated similarly. For these latter animals, refractive status, ocular length, and vitreous depth were measured before and after light treatment. Eight weeks after light treatment, retinal and sceral levels of melanopsin, melatonin receptor type (MT) 1, and mRNA protein were determined by Western blotting, real-time polymerase chain reaction (RT-PCR), and immunohistochemistry.
  
  
  RESULTS
  The level of pineal gland melatonin in the green-light group was significantly higher than that in the blue-light group. Biometric measurements showed that guinea pigs in the green-light group had a somewhat myopic refractive status. Expressions of retinal melanopsin mRNA and protein were significantly higher in the blue-light group and lower in the green-light group when compared to controls. Conversely, expressions of MT1 receptor mRNA and protein in retina and sclera were significantly higher in the green-light group and lower in the blue-light group when compared to controls.
  
  
  CONCLUSIONS
  Green light appears to suppress induction of melatonin production. In addition, 530 nm of green light is involved in the development of myopia. In the guinea pig, MT1 receptor and melanopsin appear to play roles in the development of myopia induced by 530 nm of light.
authors.affiliation: Myopia Key Lab of Health Ministry, Eye and ENT Hospital of Fudan University, Shanghai, People's Republic of China.
authors.forename: Fei
authors.initials: F
authors.lastname: Wang
authors.forename: Jiaqi
authors.initials: J
authors.lastname: Zhou
authors.forename: Yi
authors.initials: Y
authors.lastname: Lu
authors.forename: Renyuan
authors.initials: R
authors.lastname: Chu
mesh_terms: D000818:Animals; D001699:Biometry; D015153:Blotting, Western; D002851:Chromatography, High Pressure Liquid; D019084:Fluorescent Antibody Technique, Indirect; D006168:Guinea Pigs; D008027:Light; D008550:Melatonin / Q000096:biosynthesis*; D009216:Myopia / Q000209:etiology / Q000378:metabolism*; D010870:Pineal Gland / Q000378:metabolism / Q000528:radiation effects*; D012333:RNA, Messenger / Q000235:genetics; D044122:Receptor, Melatonin, MT1 / Q000235:genetics / Q000378:metabolism*; D012160:Retina / Q000378:metabolism; D042262:Retinoscopy; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017299:Rod Opsins / Q000235:genetics / Q000378:metabolism*; D012590:Sclera / Q000378:metabolism; D000099210:Melanopsin
chemical_list: D008550:Melatonin; D012333:RNA, Messenger; D044122:Receptor, Melatonin, MT1; D017299:Rod Opsins; D000099210:Melanopsin