title: Investigation of the interaction between CREB-binding protein and STAT4/STAT6.
abstract: Coactivator CBP (CREB-binding protein) has been implicated in the regulation of transcription for all signal transducer and activator of transcription factors (STATs); however, the mechanism remains unclear. Using yeast two-hybrid screening and immunoprecipitation techniques, we investigated the direct interaction of CBP with STAT4 and STAT6. The full-length CBP and five fragments of CBP (residues 1-436, 529-1200, 1-697, 967-1574 and 1678-2175) were constructed using pGBKT7 vectors, while STAT4, STAT6 and N-terminal deleted STAT4 were constructed using pGADT7 vectors. It was found that STAT4, but not STAT6, interacted directly with the 1678-2175 fragment of CBP containing the ZZ, TAZ2 and SID domain. The N-terminal of STAT4 plays an important role in this interaction since N-terminal deleted STAT4 failed to bind to any CBP fragment. The results were confirmed by immunoprecipitation using HA-tagged STAT4 or STAT6 and c-Myc tagged CBP. This work will contribute to our understanding of the mechanisms of Th cytokine imbalance.
authors.affiliation: Department of Respiratory Medicine, Chongqing Medical University, Chongqing 400014, People's Republic of China.
authors.forename: Mingxiang
authors.initials: M
authors.lastname: Zhang
authors.forename: Zhou
authors.initials: Z
authors.lastname: Fu
authors.forename: Daiyin
authors.initials: D
authors.lastname: Tian
authors.forename: Enmei
authors.initials: E
authors.lastname: Liu
authors.forename: Jihong
authors.initials: J
authors.lastname: Dai
authors.forename: Lijia
authors.initials: L
authors.lastname: Wang
mesh_terms: D000217:Acyltransferases / Q000378:metabolism; D000818:Animals; D019556:COS Cells; D050882:CREB-Binding Protein / Q000378:metabolism*; D002522:Chlorocebus aethiops; D051379:Mice; D011485:Protein Binding; D050797:STAT4 Transcription Factor / Q000378:metabolism*; D050800:STAT6 Transcription Factor / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D020798:Two-Hybrid System Techniques
chemical_list: D050797:STAT4 Transcription Factor; D050800:STAT6 Transcription Factor; D029701:Saccharomyces cerevisiae Proteins; C494100:Stat6 protein, mouse; D000217:Acyltransferases; C481660:Taz1 protein, S cerevisiae; D050882:CREB-Binding Protein; C494364:Crebbp protein, mouse