title: Pro198Leu polymorphism of GPx-1 gene and activity of erythrocytic glutathione peroxidase and lipid peroxidation products.
abstract: We studied the relationship between the GPx-1 gene Pro198Leu polymorphism genotype and activity of free-radical processes. In patients with documented CHD, the content of lipoperoxides and MDA in LDL, activity of glutathione peroxidase in erythrocytes, and genotype of GPx-1 gene Pro198Leu polymorphism were determined. In carriers of the minor allele, activity of glutathione peroxidase in erythrocytes was lower by 17% than in wild allele homozygotes, while the content of lipoperoxides and MDA in LDL was higher by 74 and 27%, respectively. We concluded than free-radical oxidation processes are more pronounced in carriers of the minor allele of GPx-1 gene Pro198Leu polymorphism.
authors.affiliation: Russian Cardiology Research and Production Complex, Federal Agency of Health Care and Social Development, Moscow, Russia.
authors.forename: Yu A
authors.initials: YA
authors.lastname: Shuvalova
authors.forename: A I
authors.initials: AI
authors.lastname: Kaminnyi
authors.forename: A N
authors.initials: AN
authors.lastname: Meshkov
authors.forename: V V
authors.initials: VV
authors.lastname: Kukharchuk
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D004912:Erythrocytes / Q000201:enzymology*; D005979:Glutathione Peroxidase / Q000097:blood / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D007930:Leucine / Q000378:metabolism*; D015227:Lipid Peroxidation; D008297:Male; D011110:Polymorphism, Genetic; D011392:Proline / Q000378:metabolism*
chemical_list: D017931:DNA Primers; D011392:Proline; D005979:Glutathione Peroxidase; D007930:Leucine