title: Genotoxic and mutagenic effects of fipronil on mice.
abstract: The toxic effects of several compounds on ecosystems are not restricted to ecological disturbances, and may also affect long-term human health. Fipronil is highly efficient in the control of pests, including those resistant to pyrethroid, organophosphate, and carbamate insecticides. Relatively little is known about the action of fipronil in vertebrates. This study was aimed to evaluate the genotoxic and mutagenic potential of this compound in mice exposed to different doses and demonstrates the damage caused by fipronil on non-target organisms in artificial conditions. Mice were divided into five groups: group I=30% of DL(50) (15 mg/kg), group II=50% of the DL(50) (25 mg/kg), group III=DL(50) (50 mg/kg), group IV=negative control, and group V=positive control. Peripheral blood was collected for the comet assay (24 h after exposure) and the micronucleus test (24, 48 and 72 h after exposure). Our findings revealed that doses of 15 mg/kg (group I) and 25 mg/kg (group II) of fipronil did not have genotoxic or mutagenic effects. Only the highest dose tested (50 mg/kg) induced DNA damage 24 h after exposure, indicating the mutagenic potential of fipronil. Therefore, this or higher doses are not recommended, as they may be toxic to non-target organisms.
authors.affiliation: Department of Biology, Institute of Biosciences, São Paulo State University, Rio Claro - SP, Brazil.
authors.forename: Patrícia Rosa
authors.initials: PR
authors.lastname: de Oliveira
authors.forename: Gervásio Henrique
authors.initials: GH
authors.lastname: Bechara
authors.forename: Sandra Eloisi
authors.initials: SE
authors.lastname: Denardi
authors.forename: Rodrigo Juliano
authors.initials: RJ
authors.lastname: Oliveira
authors.forename: Maria Izabel Camargo
authors.initials: MI
authors.lastname: Mathias
mesh_terms: D000818:Animals; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects*; D005260:Female; D007306:Insecticides / Q000008:administration & dosage / Q000633:toxicity*; D051379:Mice; D048629:Micronuclei, Chromosome-Defective / Q000139:chemically induced*; D015162:Micronucleus Tests; D009154:Mutation; D011720:Pyrazoles / Q000008:administration & dosage / Q000633:toxicity*
chemical_list: D007306:Insecticides; D011720:Pyrazoles; C082360:fipronil