title: [Effect of potassium iodide on prevention of experimental lead nephropathy and expression of nuclear factor-kappaB and fibronectin].
abstract: OBJECTIVE
  To investigate the effect of potassium iodide on the expression of nuclear factor-kappaB and fibronectin.
  
  
  METHODS
  The experiment was performed with 72 SD rats weighing about 180-220 g. The animals were randomly assigned into nine groups. Group A, B, C (n=8) served as control and were fed with distilled water for 1 month, 2 month, 3 month respectively. Group D, E, F (n=8) served as lead exposed and were fed with water with 0.5% lead acetate for 1 month, 2 month, 3 month respectively. Group G, H, I (n=8) served as potassium iodide and lead exposed and were treated with 0.5% lead acetate simultaneously taking potassium iodide 3 mg/100 g weight by intragastric administration for 1 month, 2 month, 3 month respectively. Animals of different groups were sacrificed at the end of the treatment. Ultrastructure of kidney was observed by electron microscopy; Expression of NF-kappaB and FN protein and mRNA in kidney were measured respectively by immunohistochemistry and RT-PCR.
  
  
  RESULTS
  Electron microscopic examination revealed potassium iodide could restrain the denaturalization in epithelial cells and mitochondrial cristae. The expressions of NF-kappaB protein (0.2315 +/- 0.0624, 0.3213 +/- 0.0740, 0.4729 +/- 0.0839) and mRNA (0.4370 +/- 0.0841, 0.5465 +/- 0.0503, 0.6443 +/- 0.0538) in all the lead exposed groups continuously increased compared with correspondent control groups; Group I was decreased obviously compared with group F. The expressions of FN protein (0.4243 +/- 0.0595, 0.4917 +/- 0.0891) and mRNA (0.8650 +/- 0.0880, 0.8714 +/- 0.0980) in group E and F increased compared with group B and C, but the expressions of FN protein in group I significantly decreased compared with group F; The expressions of FN mRNA in Group H and I significantly decreased compared with group E and F.
  
  
  CONCLUSIONS
  The potassium iodide can ameliorate renal ultrastructure and degrade expression of nuclear factor-kappaB and fibronectin induced by lead.
authors.affiliation: The Second Hospital of Shanxi Medical University, Taiyuan 030001, China.
authors.forename: Yu-feng
authors.initials: YF
authors.lastname: Qiao
authors.forename: Dong-zi
authors.initials: DZ
authors.lastname: Pang
authors.forename: Jin-feng
authors.initials: JF
authors.lastname: Lu
authors.forename: Bai-ying
authors.initials: BY
authors.lastname: Hu
authors.forename: Dong
authors.initials: D
authors.lastname: Liu
authors.forename: Gui-feng
authors.initials: GF
authors.lastname: Zhou
authors.forename: Bo
authors.initials: B
authors.lastname: Yang
authors.forename: Rong-shan
authors.initials: RS
authors.lastname: Li
authors.forename: Yun-sheng
authors.initials: YS
authors.lastname: Jiang
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005353:Fibronectins / Q000235:genetics / Q000378:metabolism*; D007668:Kidney / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D007674:Kidney Diseases / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D007855:Lead Poisoning / Q000150:complications / Q000378:metabolism* / Q000473:pathology; D008297:Male; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism*; D011193:Potassium Iodide / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D005353:Fibronectins; D016328:NF-kappa B; D012333:RNA, Messenger; D011193:Potassium Iodide