title: The inhibitory effects of catechins on biofilm formation by the periodontopathogenic bacterium, Eikenella corrodens.
abstract: Eikenella corrodens is a periodontopathogenic bacterium that forms biofilm even by itself. In this study, we investigated the inhibitory effects of catechins on E. corrodens biofilm formation. Biofilm formation was inhibited by the addition of 1 mM of the catechins with the pyrogallol-type B-ring and/or the galloyl group. The catechins with the galloyl group were effective at smaller doses than those with only the pyrogallol-type B-ring. An inhibitory effect was observed even when these catechins and gallic acid were added at sub-minimal inhibitory concentration (MIC) or at concentrations that showed no bactericidal effect. These results suggest that some catechins at sub-MIC might inhibit biofilm formation. No inhibitory effect of catechins at sub-MIC on biofilm formation was observed in the luxS deletion mutant. Our studies suggest that some species of catechins with the galloyl group affect autoinducer 2-mediated quorum sensing and thereby inhibit biofilm formation by E. corrodens.
authors.affiliation: Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Yamaguchi, Japan.
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Matsunaga
authors.forename: Aya
authors.initials: A
authors.lastname: Nakahara
authors.forename: Karim M
authors.initials: KM
authors.lastname: Minnatul
authors.forename: Yuichiro
authors.initials: Y
authors.lastname: Noiri
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Ebisu
authors.forename: Akio
authors.initials: A
authors.lastname: Kato
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Azakami
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects*; D002392:Catechin / Q000737:chemistry / Q000494:pharmacology*; D016124:Eikenella corrodens / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D005707:Gallic Acid / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D053038:Quorum Sensing / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D005707:Gallic Acid; D002392:Catechin