title: [Neuroprotection of sevoflurane against the ischemia injury in hippocampal slices of rat].
abstract: OBJECTIVE
  To study the protective effects and the mechanisms of sevoflurane on ischemic cerebral neurons.
  
  
  METHODS
  With electrophysiological microelectrode recoding technique, the OPS of hippocampal slices deprived with oxygen and glucose (OGD) and injured from toxicity of glutamate (Glu) in the control group, 2% sevoflurane group and 4% sevoflurane group were observed. The changes of ultrastructure in the three groups were also observed respectively.
  
  
  RESULTS
  In the control group and 2% sevoflurane group it didn't show the improvement of recovery in OPS of hippocampal slices injured from OGD and Glu. In 4% sevoflurane group the recovery degree and the recovery rate of OPS were obversely. With electricmicroscope, it was founded that in the control group and 2% sevoflurane group, the pyramidal neurons in CA1 regions deprived with glucose and oxygen and exposured by Glu were damaged. Intercellular edema were severe, the nucleus membranes were not complete, the chromatin formed mass, the endoplasmic reticulum in the cytoplasm were degenerate, mitochondrion swelled. In 4% sevoflurane group, the pyramidal neurons in CA1 regions did not swell obviously, the nucleus was clear, the nucleus membranes were complete and the mitochondria swelled lightly.
  
  
  CONCLUSIONS
  4% sevoflurane could protect hippocampal neurons deprived with glucose and oxygen from the damage. The probable mechanism is 4% sevoflurane reduced the excitatory of Glu.
authors.affiliation: Department of Anesthesiology, the Second People Hospital of Lianyungang, Lianyungang 222023, China. lylzhj2003@tom.com
authors.forename: Hong-jing
authors.initials: HJ
authors.lastname: Zhang
authors.forename: Zhi-ping
authors.initials: ZP
authors.lastname: Wang
authors.forename: Shui-qing
authors.initials: SQ
authors.lastname: Hu
authors.forename: Min
authors.initials: M
authors.lastname: Xu
authors.forename: Shan
authors.initials: S
authors.lastname: Jiang
mesh_terms: D018685:Anesthetics, Inhalation / Q000494:pharmacology; D000818:Animals; D002545:Brain Ischemia / Q000503:physiopathology*; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D006624:Hippocampus / Q000098:blood supply*; D066298:In Vitro Techniques; D008297:Male; D008738:Methyl Ethers / Q000494:pharmacology*; D018696:Neuroprotective Agents / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000517:prevention & control*; D000077149:Sevoflurane
chemical_list: D018685:Anesthetics, Inhalation; D018691:Excitatory Amino Acid Antagonists; D008738:Methyl Ethers; D018696:Neuroprotective Agents; D000077149:Sevoflurane