title: Somatostatin 14 affects the pituitary-ovarian axis in infant rats.
abstract: The effects of multiple somatostatin (SRIH-14) treatment on the pituitary-ovarian axis were examined in infant rats. Female Wistar rats received subcutaneously two daily 20 μg/100g b.w. doses for five consecutive days (from 11 to 15 days of age). Changes in cell volume, volume density and number per unit area (mm²) of follicle-stimulating (FSH), luteinizing (LH) and somatotropic (GH) immunolabeled cells were evaluated by stereology and morphometry. Serum FSH and LH concentrations were determined by RIA. Ovaries were analyzed by simple point counting of follicles. SRIH-14 treatment significantly reduced FSH and LH cell volume, while their volume density and number per unit area were unaltered. Serum concentrations of FSH and LH were significantly reduced. Volume and volume density of GH cells was significantly decreased after SRIH-14 treatment, while their number per unit area was unaltered. In the ovary, SRIH-14 induced a significant increase in the percentage of primordial follicles followed by a significant decrease in percentage of primary follicles. The number of healthy and atretic preantral follicles was unchanged. It can be concluded that SRIH-14 treatment during the infantile period markedly inhibits pituitary FSH, LH and GH cells. In the ovary, SRIH-14 acts by inhibiting initial folliculogenesis without affecting atretic processes.
authors.affiliation: Institute for Biological Research Sinisa Stanković, University of Belgrade, Belgrade, Serbia. nestorovicn.natasa@gmail.com
authors.forename: N
authors.initials: N
authors.lastname: Nestorović
authors.forename: N
authors.initials: N
authors.lastname: Ristić
authors.forename: M
authors.initials: M
authors.lastname: Manojlović-Stojanoski
authors.forename: B
authors.initials: B
authors.lastname: Sosić-Jurjević
authors.forename: S
authors.initials: S
authors.lastname: Trifunović
authors.forename: S
authors.initials: S
authors.lastname: Savin
authors.forename: V
authors.initials: V
authors.lastname: Milosević
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001835:Body Weight / Q000187:drug effects; D005260:Female; D005640:Follicle Stimulating Hormone / Q000378:metabolism; D013006:Growth Hormone / Q000378:metabolism; D007279:Injections, Subcutaneous; D007986:Luteinizing Hormone / Q000378:metabolism; D009929:Organ Size / Q000187:drug effects; D006080:Ovarian Follicle / Q000187:drug effects* / Q000378:metabolism; D010903:Pituitary Gland, Anterior / Q000187:drug effects* / Q000378:metabolism; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar; D013004:Somatostatin / Q000494:pharmacology*
chemical_list: D013004:Somatostatin; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D013006:Growth Hormone