title: [Dynamic and long-lasting expression of thrombin receptor-1 after intracerebral hemorrhage in rats].
abstract: OBJECTIVE
  To study the dynamic and long-lasting expression of thrombin receptor after acute intracerebral hemorrhage (ICH) in rats.
  
  
  METHODS
  36 rats were randomly divided into 6 groups (n = 6): Normal group and ICH model groups at 6 hours, 24 hours, 3 days, 7 days and 14 days. ICH models were produced with the induction of collagenase type VII-S. Immunohistochemical method was used to detect PAR-1 protein and RT-PCR technique was used to detect PAR-1mRNA in brain tissue around the haematoma in different groups.
  
  
  RESULTS
  PAR-1 protein and mRNA were mild positive in normal group. In model groups, intensity of PAR-1 expression started to enhance at 6 hours, and enhanced more at 24 hours. PAR-1 expression reached the peak at 3 days and began to descend. At 7 days the descent was obvious and there was further descent at 14 days. At each time point, the PAR-1 protein positive cell number and PAR-1mRNA absorbance ratio in ICH model groups were significantly higher than those in normal group (P < 0.05 or P < 0.01). In addition, PAR-1 proteins were obviously expressed in vivo in brain capillary endothelial cell.
  
  
  CONCLUSIONS
  Functional PAR-1 exists in brain capillary endothelial cells. Activation of PAR-1 after ICH due to the stimulation of thrombin is not only the initiating agent of cerebral edema after ICH, but also participates the development of cerebral edema.
authors.affiliation: Center of Neurology, Second Affiliated Hospital of Wenzhou Medical College, Wenzhou 325027, China.
authors.forename: Guo-Qing
authors.initials: GQ
authors.lastname: Zheng
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Xiao-Tong
authors.initials: XT
authors.lastname: Wang
mesh_terms: D000818:Animals; D002543:Cerebral Hemorrhage / Q000378:metabolism*; D004195:Disease Models, Animal; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D044463:Receptor, PAR-1 / Q000378:metabolism*; D013917:Thrombin / Q000378:metabolism
chemical_list: D044463:Receptor, PAR-1; D013917:Thrombin