title: Behavior of wild-type and transfected S2 cells cultured in two different media.
abstract: An animal protein-free medium composed of IPL-41 containing 6 g L(-1) yeastolate ultrafiltrate, 10 g L(-1) glucose, 2 g L(-1) lactose, 5 g L(-1) glutamine, 1% lipid emulsion, and 0.1% Pluronic F-68 was used for producing recombinant proteins in batch mode employing two cell lines, S2AcRVGP2k expressing the G glycoprotein from rabies virus (RVGP) and S2AcHBsAgHy-9C expressing the surface antigen of hepatitis B virus (HBsAg), both obtained from Drosophila melanogaster S2 cells. Growth of wild-type S2 cells was also evaluated in the same medium. Cell behavior in the tested medium was compared to that verified in Sf900 II®. The results show that in shake flasks, S2AcRVGP2k and S2AcHBsAgHy-9C cells reached around 2 × 10(7) cells mL(-1) in both media. In supplemented IPL-41 and Sf900 II® media, S2AcRVGP2k cells produced 367 ng RVGP mL(-1) and 638 ng RVGP mL(-1), respectively, while S2AcHBsAgHy-9C cells correspondently produced 573 ng HBsAg mL(-1) and 322 ng HBsAg mL(-1) in the mentioned media. In stirred tanks, S2AcRVGP2k cells reached 3 × 10(7) cells mL(-1) and produced up to 758 ng RVGP mL(-1). In general, glucose was consumed by cells, while lactate and ammonia were produced.
authors.affiliation: Departamento de Processos Biotecnológicos, Faculdade de Engenharia Química, Universidade Estadual de Campinas, Av. Albert Einstein 500, Campinas, SP, Brazil.
authors.forename: Fabiana R X
authors.initials: FR
authors.lastname: Batista
authors.forename: Kátia N
authors.initials: KN
authors.lastname: Greco
authors.forename: Renato M
authors.initials: RM
authors.lastname: Astray
authors.forename: Soraia A C
authors.initials: SA
authors.lastname: Jorge
authors.forename: Elisabeth F P
authors.initials: EF
authors.lastname: Augusto
authors.forename: Carlos A
authors.initials: CA
authors.lastname: Pereira
authors.forename: Ronaldo Z
authors.initials: RZ
authors.lastname: Mendonça
authors.forename: Angela M
authors.initials: AM
authors.lastname: Moraes
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002460:Cell Line; D016895:Culture Media, Serum-Free / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000166:cytology* / Q000254:growth & development* / Q000378:metabolism; D015870:Gene Expression; D006515:Hepatitis B virus / Q000235:genetics / Q000378:metabolism; D011820:Rabies virus / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D014162:Transfection; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D016895:Culture Media, Serum-Free; D011994:Recombinant Proteins; D014764:Viral Proteins