title: [Isolation and identification of a Banna virus strain from mosquitoes in Yunnan province].
abstract: OBJECTIVE
  To isolate and identify Banna virus (BAV) from mosquitoes collected in Mengla county of Yunnan province.
  
  
  METHODS
  Mosquito samples were collected in houses and stock yards in Mengla county, 2008. Mosquitoes were homogenized and incubated onto both C6/36 and BHK21 cells. The new isolate was identified by using ELISA and RT-PCR. The sequences of segment 5, 8 and 11 of BAV were amplified by RT-PCR and determined. Phylogenetic analysis on the new BAV were performed using MEGA4 program.
  
  
  RESULTS
  1731 mosquitoes representing 7 species were collected with one strain of BAV isolated and identified. Phylogenetic analysis based on sequences of segment 8 showed the new isolate was closed to BAV strain isolated in Yunnan, but segment 11 sequence was closed to Vietnam strain.
  
  
  CONCLUSIONS
  Results of phylogenetic analysis implied that the BAV re-assortment might have been occurred both in Chinese and Vietnam strains.
authors.affiliation: Chinese Academy of Inspection and Quarantine, Beijing 100123, China.
authors.forename: Xiao-hong
authors.initials: XH
authors.lastname: Sun
authors.forename: Xiao-long
authors.initials: XL
authors.lastname: Zhang
authors.forename: Yu-fu
authors.initials: YF
authors.lastname: Liu
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Lian
authors.initials: L
authors.lastname: Wei
authors.forename: Xu
authors.initials: X
authors.lastname: Gao
authors.forename: Wen
authors.initials: W
authors.lastname: Zhao
authors.forename: Xun
authors.initials: X
authors.lastname: Zhang
authors.forename: Xue-bing
authors.initials: XB
authors.lastname: Yang
authors.forename: Le
authors.initials: L
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002460:Cell Line; D002681:China; D018162:Coltivirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D009033:Culicidae / Q000821:virology*; D005260:Female; D017422:Sequence Analysis, DNA