title: The transphosphatidylation potential of a membrane-bound phospholipase D from poppy seedlings.
abstract: Plant phospholipases D (PLDs) occur in a large variety of isoenzymes, which differ in Ca(2+) ion requirement, phosphatidylinositol-4,5-bisphosphate (PIP(2)) activation and substrate selectivity. In the present study a membrane-bound PLD has been identified in the microsomal fractions of poppy seedlings (Papaver somniferum). The maximum PLD activity is found after 2 days of germination in endosperms and after 3 days in developing seedlings. In contrast to the four poppy PLD isoenzymes described hitherto, the membrane-bound form is active at lower Ca(2+) ion concentrations (in the micromolar instead of millimolar range) and needs PIP(2) for hydrolytic activity. Remarkable differences are also observed in head group exchange reactions. The reaction rates of the transphosphatidylation of phosphatidylcholine by various acceptor alcohols follow the sequence glycerol>serine>myo-inositol>ethanolamine, whereas ethanolamine is preferred by most other PLDs. Despite the biocatalytic differences, the membrane-bound PLD interacts with polyclonal antibodies raised against α-type PLD, which reveals some structural similarities between these two enzymes.
authors.affiliation: Department of Cell and Molecular Biology of Drugs, Faculty of Pharmacy, Comenius University, Bratislava, Slovakia.
authors.forename: Marek
authors.initials: M
authors.lastname: Oblozinsky
authors.forename: Lydia
authors.initials: L
authors.lastname: Bezakova
authors.forename: Johanna
authors.initials: J
authors.lastname: Mansfeld
authors.forename: Renate
authors.initials: R
authors.lastname: Ulbrich-Hofmann
mesh_terms: D002118:Calcium / Q000378:metabolism; D002384:Catalysis; D002462:Cell Membrane / Q000201:enzymology*; D006868:Hydrolysis; D007527:Isoenzymes / Q000378:metabolism; D015394:Molecular Structure; D010207:Papaver / Q000201:enzymology* / Q000254:growth & development; D019269:Phosphatidylinositol 4,5-Diphosphate / Q000378:metabolism; D010739:Phospholipase D / Q000378:metabolism*; D011485:Protein Binding / Q000235:genetics; D036226:Seedlings / Q000201:enzymology
chemical_list: D007527:Isoenzymes; D019269:Phosphatidylinositol 4,5-Diphosphate; D010739:Phospholipase D; D002118:Calcium