title: Photophysical mechanisms of collagen modification by 80 MHz femtosecond laser.
abstract: Photophysical mechanisms of collagen photomodification (CFP) by the use of a 80 MHz, 780 nm femtosecond titanium-sapphire laser were investigated. Our observation that the decrease in collagen second harmonic generation and increase in two-photon autofluorescence intensity occurred primarily at sites where photoproducts were present suggested that the photoproducts may act to facilitate the CFP process. Laser power study of CFP indicated that the efficiency of the process depended on the sixth power of the laser intensity. Furthermore, it was demonstrated that CFP can be used for bending and cutting of collagen fibers and creating 3D patterns within collagen matrix with high precision (~2 μm).
authors.affiliation: Department of Physics, National Taiwan University, Taipei 106, Taiwan.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Hovhannisyan
authors.forename: Ara
authors.initials: A
authors.lastname: Ghazaryan
authors.forename: Yung-Fang
authors.initials: YF
authors.lastname: Chen
authors.forename: Shean-Jen
authors.initials: SJ
authors.lastname: Chen
authors.forename: Chen-Yuan
authors.initials: CY
authors.lastname: Dong
mesh_terms: D000818:Animals; D002356:Cartilage / Q000033:anatomy & histology; D002417:Cattle; D002645:Chickens; D003094:Collagen / Q000737:chemistry* / Q000528:radiation effects*; D020405:Dermis / Q000033:anatomy & histology; D007700:Kinetics; D007834:Lasers; D008027:Light; D055668:Photochemical Processes / Q000528:radiation effects*; D051381:Rats; D013710:Tendons / Q000033:anatomy & histology; D013997:Time Factors
chemical_list: D003094:Collagen