title: [Protective effect of Quinacrine on striatum neurons from heat treatment injury].
abstract: OBJECTIVE
  To study the protective effect of Quinacrine(QA) on rat striatum neurons from the injury caused by heat environment treatment, to probe the relationship between cell membrane injury and cellular injury protection, and to seek the possibility of QA as a preventive agent to heat injury.
  
  
  METHODS
  Primary cultured striatum neurons from newborn rats were pretreated with QA at different concentration for 1 h, and then heat-treated at 43 degrees C for another 1 h. Cell necrosis was detected by Trypan blue staining, and apoptosis was evaluated through Activated Caspase-3 dye and TdT dye.
  
  
  RESULTS
  Heat treatment effected the survival of striatum neurons and resulted in great number of cell death, which was mainly mediated by cell necrosis process. It was shown that treatment of QA itself had little effect on the survival of striatum neurons, while QA pretreatment decreased cellular necrosis caused by following heat treatment.
  
  
  CONCLUSIONS
  QA protects striatum neurons from heat environment injury at about 20 pmol/L, and the protection may mediated by reduction of necrosis.
authors.affiliation: Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Yong-Qi
authors.initials: YQ
authors.lastname: Zhao
authors.forename: Yan
authors.initials: Y
authors.lastname: Wu
authors.forename: Shu-Hong
authors.initials: SH
authors.lastname: Liu
authors.forename: Xue-Ming
authors.initials: XM
authors.lastname: Ge
authors.forename: Ai-Shi
authors.initials: AS
authors.lastname: Ding
authors.forename: Ming
authors.initials: M
authors.lastname: Fan
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000378:metabolism; D016923:Cell Death / Q000187:drug effects; D002478:Cells, Cultured; D003342:Corpus Striatum / Q000166:cytology*; D018869:Heat-Shock Response; D009474:Neurons / Q000187:drug effects*; D011796:Quinacrine / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D053148:Caspase 3; D011796:Quinacrine