title: [Effects of protein kinase CK2α on apoptosis and ultrastructure of laryngeal carcinoma cells].
abstract: OBJECTIVE
  To investigate the effect of protein kinase CK2α on apoptosis and ultrastructure of human laryngeal carcinoma cells and its possible mechanism.
  
  
  METHODS
  The siRNA expression plasmid psiRNA-hH1neo-CK2α specific to protein kinase CK2α and non-specific siRNA expression plasmid psiRNA-hH1neo-cont were transfected into Hep-2 cells respectively by lipofectamine method. Western blot was used to detect the expression of kinase CK2α protein. The apoptotic rate was measured by Annexin V-FITC/PI double-staining. The morphological changes of Hep-2 cells were observed under transmission electron microscope (TEM). The expressions of bcl-2 and Bax protein were measured by Western blot.
  
  
  RESULTS
  The expression of protein kinase CK2α protein significantly decreased in the Hep-2 cells transfected with psiRNA-hH1neo-CK2α (P < 0.01). Compared with the untransfected cells and siRNA-hH1neo-cont transfected group, psiRNA-hH1neo-CK2α transfected group presented with classical ultrastructural features of apoptosis, such as karyopyknosis, chromatic agglutination adjacent to nuclear membrane and apoptotic body. The apoptotic rate of psiRNA-hH1neo-CK2α transfected group was obviously higher than that in untransfected cells and siRNA-hH1neo-cont transfected group (25.66% ± 0.83% vs 3.66% ± 0.43%, 5.18% ± 0.22%, both P < 0.05). Compared with two other groups, the bcl-2 protein expression of psiRNA-hH1neo-CK2α transfected group decreased (0.20 ± 0.09 vs 0.72 ± 0.16, 0.56 ± 0.11, both P < 0.01), the Bax protein expression increased (0.81 ± 0.17 vs 0.26 ± 0.12, 0.33 ± 0.17, both P < 0.01) while the ratio of bcl-2 to Bax decreased (0.25 ± 0.05 vs 2.76 ± 0.21, 1.70 ± 0.22, both P < 0.01).
  
  
  CONCLUSIONS
  Protein kinase CK2a plays an important role in the apoptosis of human laryngeal carcinoma cells possibly by decreasing bcl-2/Bax. Protein kinase CK2a may provide a potential therapeutic target against human laryngeal carcinoma.
authors.affiliation: Department of Otorhinolaryngology, Capital Medical University, Beijing 100020, China.
authors.forename: Jian-Ting
authors.initials: JT
authors.lastname: Wang
authors.forename: Shu-Sheng
authors.initials: SS
authors.lastname: Gong
mesh_terms: D017209:Apoptosis; D047390:Casein Kinase II / Q000235:genetics*; D045744:Cell Line, Tumor; D006801:Humans; D007822:Laryngeal Neoplasms / Q000473:pathology* / Q000648:ultrastructure*; D034741:RNA, Small Interfering / Q000235:genetics*; D014162:Transfection
chemical_list: D034741:RNA, Small Interfering; D047390:Casein Kinase II