title: The CYPome of Sorangium cellulosum So ce56 and identification of CYP109D1 as a new fatty acid hydroxylase.
abstract: The first systematic study of the complete cytochrome P450 complement (CYPome) of Sorangium cellulosum So ce56, which is a producer of important secondary metabolites and has the largest bacterial genome sequenced to date, is presented. We describe the bioinformatic analysis of the So ce56 cytochrome P450 complement consisting of 21 putative P450 genes. Because fatty acids play a pivotal role during the complex life cycle of myxobacteria, we focused our studies on the characterization of fatty acid hydroxylases. Three novel potential fatty acid hydroxylases (CYP109D1, CYP264A1, and CYP266A1) were used for detailed characterization. One of them, CYP109D1 was able to perform subterminal hydroxylation of saturated fatty acids with the support of two autologous and one heterologous electron transfer system(s). The kinetic parameters for the product hydroxylation were derived.
authors.affiliation: Department of Biochemistry, Saarland University, Campus B2.2, 66123 Saarbrücken, Germany.
authors.forename: Yogan
authors.initials: Y
authors.lastname: Khatri
authors.forename: Frank
authors.initials: F
authors.lastname: Hannemann
authors.forename: Kerstin M
authors.initials: KM
authors.lastname: Ewen
authors.forename: Dominik
authors.initials: D
authors.lastname: Pistorius
authors.forename: Olena
authors.initials: O
authors.lastname: Perlova
authors.forename: Norio
authors.initials: N
authors.lastname: Kagawa
authors.forename: Alexander O
authors.initials: AO
authors.lastname: Brachmann
authors.forename: Rolf
authors.initials: R
authors.lastname: Müller
authors.forename: Rita
authors.initials: R
authors.lastname: Bernhardt
mesh_terms: D019295:Computational Biology; D003577:Cytochrome P-450 Enzyme System / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism*; D006900:Hydroxylation; D007700:Kinetics; D006899:Mixed Function Oxygenases / Q000235:genetics / Q000378:metabolism*; D009231:Myxococcales / Q000201:enzymology*; D011485:Protein Binding; D013379:Substrate Specificity
chemical_list: D005227:Fatty Acids; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases