title: Ovine pulmonary adenocarcinoma as an animal model of progressive lung cancer and the impact of nutritional selenium supply.
abstract: Jaagsiekte sheep retrovirus (JSRV) is known to induce ovine pulmonary adenocarcinoma (OPA). Several studies have suggested an influence of selenium (Se) status on cancer progression. Thus, combining OPA with a defined Se supply might serve as a suitable animal model to study the impact of Se on lung cancer progression. 16 naturally JSRV-infected sheep were divided into 2 treatment groups receiving (a) <0.05 and (b) 0.2 mg Se/kg dry matter in diet, respectively. Computed tomography (CT) was performed repeatedly and evaluated using a CT-OPA-score system. Liver biopsies were taken three-monthly, blood samples were collected biweekly to study treatment effects on Se concentrations and glutathione peroxidase (GPx) activity. Cell pellets from bronchoalveolar lavage fluid (BALF) were tested for JSRV by PCR to approve the infection. To date, four animals of the ongoing study have been euthanised. Autopsy and histopathology were performed and correlated to CT analysis. JSRV was detected in BALF cell pellets. Progression of lung tumours was monitored successfully by repeated CT examinations, enabling the detection of even small nodules or increased lung density. Histopathology revealed bronchioloalveolar adenocarcinoma in lung areas suspicious to be OPA from CT evaluation. Score-based analysis of CT images for quantifying tumour progression proved as a valuable tool. Se concentration and GPx activity increased in liver and serum of group b and verified the efficiency of different feeding regime. In conclusion, OPA along with CT, autopsy/histopathology, trace element and enzyme activity analysis provide a suitable large animal model to examine the impact of Se supply on lung tumourigenesis.
authors.affiliation: Clinic for Swine, Small Ruminants, Forensic Medicine and Ambulatory Service, University of Veterinary Medicine Hannover, Bischofsholer Damm 15, D-30173 Hannover, Germany. esther.humann@tiho-hannover.de
authors.forename: Esther
authors.initials: E
authors.lastname: Humann-Ziehank
authors.forename: Petra
authors.initials: P
authors.lastname: Wolf
authors.forename: Kostja
authors.initials: K
authors.lastname: Renko
authors.forename: Lutz
authors.initials: L
authors.lastname: Schomburg
authors.forename: Michael
authors.initials: M
authors.lastname: Ludwig Bruegmann
authors.forename: Arnim
authors.initials: A
authors.lastname: Andreae
authors.forename: Carsten
authors.initials: C
authors.lastname: Brauer
authors.forename: Martin
authors.initials: M
authors.lastname: Ganter
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D018450:Disease Progression; D005979:Glutathione Peroxidase / Q000097:blood; D008099:Liver / Q000378:metabolism; D008175:Lung Neoplasms / Q000000981:diagnostic imaging / Q000178:diet therapy* / Q000473:pathology*; D011648:Pulmonary Adenomatosis, Ovine / Q000000981:diagnostic imaging / Q000178:diet therapy* / Q000473:pathology*; D012643:Selenium / Q000008:administration & dosage* / Q000097:blood / Q000627:therapeutic use*; D012756:Sheep; D014057:Tomography, X-Ray Computed
chemical_list: D005979:Glutathione Peroxidase; D012643:Selenium