title: Label-free antibody detection using band edge fringes in SOI planar photonic crystal waveguides in the slow-light regime.
abstract: We report experimental results of label-free anti-bovine serum albumin (anti-BSA) antibody detection using a SOI planar photonic crystal waveguide previously bio-functionalized with complementary BSA antigen probes. Sharp fringes appearing in the slow-light regime near the edge of the guided band are used to perform the sensing. We have modeled the presence of these band edge fringes and demonstrated the possibility of using them for sensing purposes by performing refractive index variations detection, achieving a sensitivity of 174.8 nm/RIU. Then, label-free anti-BSA biosensing experiments have been carried out, estimating a surface mass density detection limit below 2.1 pg/mm2 and a total mass detection limit below 0.2 fg.
authors.affiliation: Nanophotonics Technology Center, Universidad Politécnica de Valencia, Camino de Vera s/n, Valencia, Spain. jaigarru@ntc.upv.es
authors.forename: Jaime
authors.initials: J
authors.lastname: García-Rupérez
authors.forename: Veronica
authors.initials: V
authors.lastname: Toccafondo
authors.forename: María José
authors.initials: MJ
authors.lastname: Bañuls
authors.forename: Javier García
authors.initials: JG
authors.lastname: Castelló
authors.forename: Amadeu
authors.initials: A
authors.lastname: Griol
authors.forename: Sergio
authors.initials: S
authors.lastname: Peransi-Llopis
authors.forename: Ángel
authors.initials: Á
authors.lastname: Maquieira
mesh_terms: D000818:Animals; D000906:Antibodies / Q000032:analysis*; D015374:Biosensing Techniques / Q000295:instrumentation*; D002417:Cattle; D003198:Computer Simulation; D003460:Crystallization; D008855:Microscopy, Electron, Scanning; D017785:Photons; D012031:Refractometry; D012710:Serum Albumin, Bovine / Q000276:immunology*; D012825:Silicon / Q000737:chemistry*; D013194:Staining and Labeling; D013997:Time Factors
chemical_list: D000906:Antibodies; D012710:Serum Albumin, Bovine; D012825:Silicon