title: Angiopoietin-2 inhibits the growth of tongue carcinoma without affecting expression of vascular endothelial growth factor.
abstract: Angiopoietin-2 (Ang-2) has been identified as an important factor in tumour angiogenesis through its action in blocking the stabilizing actions of Ang-2 and leading to new tumour vessel growth in the presence of vascular endothelial growth factor (VEGF). In the authors' previous study, over-expression of Ang-2 in Tca8113 tongue tumour cells inhibited growth. The current study aims to clarify the mechanisms of Ang-2-mediated tumour growth inhibition and its role in the regulation of VEGF expression. These studies used tumours derived from Ang-2-transfected Tca8113 cells injected into nude mice. The results showed that Ang-2-transfected tumours displayed aberrant angiogenic vessels with few associated smooth muscle cells. No detectable differences in VEGF expression were observed between Ang-2-transfected and parental tumours. Tumours produced by the Ang-2 transfection also had a higher apoptosis index and lower tumour cell proliferative index than tumours in the control groups. These observations suggest that enhanced expression of Ang-2 has no effect on VEGF expression and results in tumour vessel regression and inhibition of tumour growth.
authors.affiliation: Department of Head-Neck Otolaryngology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang Province 310003, China. jianjiang@zju.edu.cn
authors.forename: H-h
authors.initials: HH
authors.lastname: Chen
authors.forename: L
authors.initials: L
authors.lastname: Chai
authors.forename: S-q
authors.initials: SQ
authors.lastname: Wang
authors.forename: Z-j
authors.initials: ZJ
authors.lastname: Shi
authors.forename: Q-l
authors.initials: QL
authors.lastname: Wu
mesh_terms: D000199:Actins / Q000032:analysis; D042702:Angiopoietin-2 / Q000235:genetics / Q000502:physiology*; D000818:Animals; D017209:Apoptosis / Q000502:physiology; D002277:Carcinoma / Q000098:blood supply / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D042783:Endothelial Cells / Q000473:pathology; D004730:Endothelium, Vascular / Q000473:pathology; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D008055:Lipids; D008081:Liposomes; D051379:Mice; D008819:Mice, Nude; D055806:Microvessels / Q000473:pathology; D009131:Muscle, Smooth, Vascular / Q000473:pathology; D032389:Myocytes, Smooth Muscle / Q000473:pathology; D009368:Neoplasm Transplantation; D009389:Neovascularization, Pathologic / Q000473:pathology; D010957:Plasmids / Q000235:genetics; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014062:Tongue Neoplasms / Q000098:blood supply / Q000473:pathology*; D014162:Transfection; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis / Q000502:physiology*
chemical_list: D000199:Actins; D042702:Angiopoietin-2; D008055:Lipids; C086724:Lipofectamine; D008081:Liposomes; D018809:Proliferating Cell Nuclear Antigen; D042461:Vascular Endothelial Growth Factor A; C467480:vascular endothelial growth factor A, mouse