title: Chronic administration of ethanol leads to an increased incidence of hepatocellular adenoma by promoting H-ras-mutated cells.
abstract: This study used tissue samples from male B6C3F1 mice treated with ethanol in drinking water (0%, 2.5%, or 5%) for 4 or 104 weeks. We tested whether chronic alcohol drinking promotes oxidative stress in the liver and characterized the mutation profile of spontaneous and ethanol-induced tumors. We show that ethanol does not cause detectable oxidative stress in the liver at any time point and acts by promoting H-ras mutated cells.
authors.affiliation: Department of Environmental Sciences and Engineering, University of North Carolina, Chapel Hill, 27599-7431, United States.
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Jeannot
authors.forename: Igor P
authors.initials: IP
authors.lastname: Pogribny
authors.forename: Frederick A
authors.initials: FA
authors.lastname: Beland
authors.forename: Ivan
authors.initials: I
authors.lastname: Rusyn
mesh_terms: D018248:Adenoma, Liver Cell / Q000139:chemically induced / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002492:Central Nervous System Depressants / Q000008:administration & dosage / Q000633:toxicity; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000008:administration & dosage / Q000633:toxicity*; D005787:Gene Frequency; D006519:Hepatitis, Alcoholic / Q000209:etiology / Q000473:pathology; D007150:Immunohistochemistry; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced / Q000235:genetics* / Q000378:metabolism; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D016296:Mutagenesis / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D013997:Time Factors; D051176:beta Catenin / Q000378:metabolism; D018631:ras Proteins / Q000235:genetics*
chemical_list: D002492:Central Nervous System Depressants; D018809:Proliferating Cell Nuclear Antigen; D051176:beta Catenin; D000431:Ethanol; D018631:ras Proteins