title: [The state of SHP-1 and CD45 in lymphocyte under high partial pressure of oxygen].
abstract: OBJECTIVE
  To explore the function state of protein tyrosine phosphatase (PTP) SHP-1 and CD45 under high partial pressure of oxygen (Po2) in lymphocyte.
  
  
  METHODS
  Primarily cultured rat splenic lymphocytes were treated with different pressure-duration of oxygen that led to promotion or inhibition of cell function. The catalytic activities, protein expression and tyrosine phosphorylation of SHP-1 and CD45 were determined.
  
  
  RESULTS
  The activity of SHP-1 was decreased after high Po2 treatment no matter what pressure-duration was, while the activity of CD45 was decreased only after high PoF that led to inhibition of lymphocyte function. The proteins expressed and tyrosine phosphorylation levels of two enzymes had no alteration in most treatment groups.
  
  
  CONCLUSIONS
  The decrease of PTP catalytic activities might be caused by their structures modification by high Po2 induced reactive oxygen species. SHP-1 and CD45 might be one of the key points of action through which high PO2 exerts its effects on lymphocytes.
authors.affiliation: Department of Nautical Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai 200433, China.
authors.forename: Wei-gang
authors.initials: WG
authors.lastname: Xu
authors.forename: Heng-yi
authors.initials: HY
authors.lastname: Tao
authors.forename: Yun
authors.initials: Y
authors.lastname: Liu
authors.forename: Chun-lei
authors.initials: CL
authors.lastname: Jiang
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D017493:Leukocyte Common Antigens / Q000378:metabolism*; D008214:Lymphocytes / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D010100:Oxygen / Q000378:metabolism*; D010313:Partial Pressure; D010766:Phosphorylation; D053824:Protein Tyrosine Phosphatase, Non-Receptor Type 6 / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism*
chemical_list: D017382:Reactive Oxygen Species; D017493:Leukocyte Common Antigens; D053824:Protein Tyrosine Phosphatase, Non-Receptor Type 6; C509250:Ptpn6 protein, rat; C516771:Ptprc protein, rat; D010100:Oxygen