title: Lectins from the Red Marine Algal Species Bryothamnion seaforthii and Bryothamnion triquetrum as Tools to Differentiate Human Colon Carcinoma Cells.
abstract: The carbohydrate-binding activity of the algal lectins from the closely related red marine algal species Bryothamnion triquetrum (BTL) and Bryothamnion seaforthii (BSL) was used to differentiate human colon carcinoma cell variants with respect to their cell membrane glyco-receptors. These lectins interacted with the cells tested in a dose-dependent manner. Moreover, the fluorescence spectra of both lectins clearly differentiated the cells used as shown by FACS profiles. Furthermore, as observed by confocal microscopy, BTL and BSL bound to cell surface glycoproteins underwent intense internalization, which makes them possible tools in targeting strategies.
authors.affiliation: Curso de Medicina/Campus de Sobral, Universidade Federal do Ceará, Fortaleza 60020-181, Brazil.
authors.forename: Vicente P T
authors.initials: VP
authors.lastname: Pinto
authors.forename: Henri
authors.initials: H
authors.lastname: Debray
authors.forename: Danuta
authors.initials: D
authors.lastname: Dus
authors.forename: Edson H
authors.initials: EH
authors.lastname: Teixeira
authors.forename: Taianá Maia
authors.initials: TM
authors.lastname: de Oliveira
authors.forename: Victor Alves
authors.initials: VA
authors.lastname: Carneiro
authors.forename: Alrieta H
authors.initials: AH
authors.lastname: Teixeira
authors.forename: Gerardo C
authors.initials: GC
authors.lastname: Filho
authors.forename: Celso S
authors.initials: CS
authors.lastname: Nagano
authors.forename: Kyria S
authors.initials: KS
authors.lastname: Nascimento
authors.forename: Alexandre H
authors.initials: AH
authors.lastname: Sampaio
authors.forename: Benildo S
authors.initials: BS
authors.lastname: Cavada