title: Photothermal nanoblade for patterned cell membrane cutting.
abstract: We report a photothermal nanoblade that utilizes a metallic nanostructure to harvest short laser pulse energy and convert it into a highly localized and specifically shaped explosive vapor bubble. Rapid bubble expansion and collapse punctures a lightly-contacting cell membrane via high-speed fluidic flows and induced transient shear stress. The membrane cutting pattern is controlled by the metallic nanostructure configuration, laser pulse polarization, and energy. Highly controllable, sub-micron sized circular hole pairs to half moon-like, or cat-door shaped, membrane cuts were realized in glutaraldehyde treated HeLa cells.
authors.affiliation: Department of Electrical Engineering, University of California, Los Angeles, Los Angeles, CA 90095, USA.
authors.forename: Ting-Hsiang
authors.initials: TH
authors.lastname: Wu
authors.forename: Tara
authors.initials: T
authors.lastname: Teslaa
authors.forename: Michael A
authors.initials: MA
authors.lastname: Teitell
authors.forename: Pei-Yu
authors.initials: PY
authors.lastname: Chiou
mesh_terms: D000042:Absorption; D002462:Cell Membrane / Q000737:chemistry*; D003198:Computer Simulation; D006367:HeLa Cells; D006801:Humans; D007834:Lasers; D008027:Light; D049329:Nanostructures / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D010363:Pattern Recognition, Automated / Q000379:methods*; D015203:Reproducibility of Results; D013696:Temperature