title: Redistribution of tight junction proteins during EPEC infection in vivo.
abstract: Enteropathogenic Escherichia coli (EPEC) is a leading cause of diarrhea among infants. Tight junction plays a vital role in intestinal paracellular permeability by forming physical intercellular barriers in epithelial cells. However, the impact of this enteric pathogen on tight junctions in vivo has not been fully investigated. In the present study, the alterations in tight junctions following EPEC infection in vivo were investigated. Western blot analysis revealed that the tight junction proteins, occludin and claudin-1, were displaced from tight junction membrane microdomains to Triton X-100 soluble fractions after EPEC infection. Changes in intestinal paracellular permeability were determined using the molecular tracer biotin, which was observed to penetrate the epithelia and extended into the lamina propria, indicating disruption in tight junction barrier function. Our results suggested that redistribution of tight junction proteins plays an important role in the disruption of epithelial barrier function induced by EPEC infection, which may provide new insight into the pathogenesis of diarrhea caused by EPEC.
authors.affiliation: Department of Surgery, Jinling Hospital, Nanjing University School of Medicine, Nanjing, 210002, People's Republic of China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhang
authors.forename: Qiurong
authors.initials: Q
authors.lastname: Li
authors.forename: Chenyang
authors.initials: C
authors.lastname: Wang
authors.forename: Ning
authors.initials: N
authors.lastname: Li
authors.forename: Jieshou
authors.initials: J
authors.lastname: Li
mesh_terms: D000818:Animals; D002463:Cell Membrane Permeability; D062445:Claudin-1; D003967:Diarrhea; D054308:Enteropathogenic Escherichia coli; D004847:Epithelial Cells / Q000378:metabolism; D004927:Escherichia coli Infections / Q000378:metabolism* / Q000473:pathology; D007413:Intestinal Mucosa / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology*; D007421:Intestine, Small / Q000378:metabolism / Q000473:pathology; D008297:Male; D021962:Membrane Microdomains; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D062793:Occludin; D021381:Protein Transport; D019108:Tight Junctions / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology*
chemical_list: D062445:Claudin-1; C568113:Cldn1 protein, mouse; D008565:Membrane Proteins; D062793:Occludin; C568986:Ocln protein, mouse