title: [The protective effect of puerarin on cultured rat cerebral cortical astrocytes].
abstract: OBJECTIVE
  To study the effects of puerarin (Pue) on impairment of rat astrocytes in primary cell culture induced by oxygen-glucose deprivation (OGD) or sodium glutamate (Glu).
  
  
  METHODS
  Astrocyte damage induced by (OGD), Glu or (+/-)-1-aminocyclopentane-trans-1,3-dicarboxylic acid (trans-ACPD), as well as the action of Pue was measured by determining the intracellular water space (as measured by 3-O-methyl-[1- 3H]D-glucose uptake) of astrocytes and the leakage of lactate dehydrogenase (LDH) from astrocytes.
  
  
  RESULTS
  Following the exposure to OGD for 5 h, 0.5 mmol/L Glu or 1 mmol/L trans-ACPD for 1 h, the astrocyte volume and LDH leakage from astrocytes were increased. 0.1 mmol/L Pue, when co-incubated with OGD, Glu or trans-ACPD, reduced astrocytic swelling and the LDH leakage.
  
  
  CONCLUSIONS
  Pue had protective effects on astrocytes damaged by OGD, Glu or trans-ACPD.
authors.affiliation: Beijing Neurosurgical Institute, Beijing 100050, China.
authors.forename: L P
authors.initials: LP
authors.lastname: Dong
authors.forename: M
authors.initials: M
authors.lastname: Han
authors.forename: F
authors.initials: F
authors.lastname: Yuan
authors.forename: L X
authors.initials: LX
authors.lastname: Xu
authors.forename: T Y
authors.initials: TY
authors.lastname: Wang
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology; D015687:Cell Hypoxia; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology / Q000187:drug effects; D003515:Cycloleucine / Q000031:analogs & derivatives / Q000633:toxicity; D004336:Drug Antagonism; D005947:Glucose / Q000494:pharmacology; D018698:Glutamic Acid / Q000633:toxicity; D007529:Isoflavones / Q000494:pharmacology*; D018696:Neuroprotective Agents / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
chemical_list: D007529:Isoflavones; D018696:Neuroprotective Agents; D003515:Cycloleucine; C036361:1-amino-1,3-dicarboxycyclopentane; D018698:Glutamic Acid; D005947:Glucose; C033607:puerarin