title: Lipid raft proteome reveals that oxidative phosphorylation system is associated with the plasma membrane.
abstract: Although accumulating proteomic analyses have supported the fact that mitochondrial oxidative phosphorylation (OXPHOS) complexes are localized in lipid rafts, which mediate cell signaling, immune response and host-pathogen interactions, there has been no in-depth study of the physiological functions of lipid-raft OXPHOS complexes. Here, we show that many subunits of OXPHOS complexes were identified from the lipid rafts of human adipocytes, C2C12 myotubes, Jurkat cells and surface biotin-labeled Jurkat cells via shotgun proteomic analysis. We discuss the findings of OXPHOS complexes in lipid rafts, the role of the surface ATP synthase complex as a receptor for various ligands and extracellular superoxide generation by plasma membrane oxidative phosphorylation complexes.
authors.affiliation: College of Life Sciences and Biotechnology, Korea University, 1, 5-ka, Anam-dong, Sungbuk-ku, Seoul, Korea.
authors.forename: Bong-Woo
authors.initials: BW
authors.lastname: Kim
authors.forename: Chang Seok
authors.initials: CS
authors.lastname: Lee
authors.forename: Jae-Sung
authors.initials: JS
authors.lastname: Yi
authors.forename: Joo-Hyung
authors.initials: JH
authors.lastname: Lee
authors.forename: Joong-Won
authors.initials: JW
authors.lastname: Lee
authors.forename: Hyo-Jung
authors.initials: HJ
authors.lastname: Choo
authors.forename: Soon-Young
authors.initials: SY
authors.lastname: Jung
authors.forename: Min-Sik
authors.initials: MS
authors.lastname: Kim
authors.forename: Sang-Won
authors.initials: SW
authors.lastname: Lee
authors.forename: Myung-Shik
authors.initials: MS
authors.lastname: Lee
authors.forename: Gyesoon
authors.initials: G
authors.lastname: Yoon
authors.forename: Young-Gyu
authors.initials: YG
authors.lastname: Ko
mesh_terms: D025181:ATP Synthetase Complexes / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000096:biosynthesis; D000818:Animals; D002462:Cell Membrane / Q000201:enzymology*; D006801:Humans; D021962:Membrane Microdomains / Q000201:enzymology*; D051379:Mice; D051336:Mitochondrial Membranes / Q000201:enzymology; D009097:Multienzyme Complexes / Q000378:metabolism*; D010085:Oxidative Phosphorylation; D020543:Proteome / Q000378:metabolism*; D013481:Superoxides / Q000378:metabolism
chemical_list: D009097:Multienzyme Complexes; D020543:Proteome; D013481:Superoxides; D000255:Adenosine Triphosphate; D025181:ATP Synthetase Complexes