title: Labeling of mesenchymal stem cells with bioconjugated quantum dots.
abstract: Quantum dots (QDs) are semiconductor nanocrystals, and recently they have been shown as effective probes for cell labeling. Due to their unique spectral, physical, and chemical properties, QDs can concurrently tag multiple intercellular and intracellular components of live cells for time ranging from seconds to months. Different color QDs can label different cell components that can be visualized with fluorescent microscopy or in vivo. Here, we provide a detailed protocol for labeling postnatal and differentiated stem/progenitor cells with bioconjugated quantum dots. For example, peptide CGGGRGD is immobilized on CdSe-ZnS QDs with free carboxyl groups. These bioconjugates label selected integrins on cell membrane of human mesenchymal stem cells (hMSCs). QD concentration and incubation time to effectively label hMSCs is optimized. We discovered that bioconjugated QDs effectively label hMSCs not only during population doubling, but also during multi-lineage differentiation into osteoblasts, chondrocytes, and adipocytes. Undifferentiated and differentiated stem cells labeled with bioconjugated QDs can be readily imaged by fluorescent microscopy. Thus, quantum dots represent an effective cell labeling probe and an alternative to organic dyes and fluorescent proteins for cell labeling and cell tracking.
authors.affiliation: Tissue Engineering and Regenerative Medicine Laboratory, Department of Biomedical Engineering, College of Dental Medicine, Columbia University Medical Center, New York, NY 10032, USA. bs2315@columbia.edu
authors.forename: Bhranti S
authors.initials: BS
authors.lastname: Shah
authors.forename: Jeremy J
authors.initials: JJ
authors.lastname: Mao
mesh_terms: D002454:Cell Differentiation / Q000502:physiology; D006801:Humans; D016023:Integrins / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000648:ultrastructure*; D008856:Microscopy, Fluorescence; D010455:Peptides / Q000378:metabolism; D045663:Quantum Dots; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D016023:Integrins; D010455:Peptides