title: The expression of peroxisomal protein transcripts increased by retinoic acid during neural differentiation.
abstract: Peroxisomal matrix protein is mainly expressed in heart, skeletal muscle, and brain tissues. To study the expression of peroxisomal protein (PEP) during neurogenesis, we employed mouse embryonic carcinoma cells (P19) and embryonic stem cells (mESCs) as an in vitro model for neural differentiation by retinoic acid (RA) induction. Expression pattern of PEP was investigated under distinct steps of differentiation by RT-PCR and real-time PCR. The results revealed that expression of PEP transcripts was markedly increased after the RA treatment at embryoid body and neural stages. Therefore, we concluded that PEP might be involved in the early process of neurogenesis, which needs further verification.
authors.affiliation: Basic Medical Sciences Department, Islamic Azad University, Khorasgan Branch, Isfahan, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Ostadsharif
authors.forename: Kamran
authors.initials: K
authors.lastname: Ghaedi
authors.forename: Mohammad
authors.initials: M
authors.lastname: Hossein Nasr-Esfahani
authors.forename: Marzieh
authors.initials: M
authors.lastname: Mojbafan
authors.forename: Somayeh
authors.initials: S
authors.lastname: Tanhaie
authors.forename: Khadijeh
authors.initials: K
authors.lastname: Karbalaie
authors.forename: Hossein
authors.initials: H
authors.lastname: Baharvand
mesh_terms: D000818:Animals; D002374:Catalase / Q000235:genetics; D045744:Cell Line, Tumor; D054278:Embryonal Carcinoma Stem Cells / Q000187:drug effects; D020869:Gene Expression Profiling; D051379:Mice; D008810:Mice, Inbred C57BL; D055495:Neurogenesis / Q000187:drug effects* / Q000235:genetics; D020675:Peroxisomes / Q000187:drug effects* / Q000235:genetics; D014158:Transcription, Genetic / Q000187:drug effects*; D014212:Tretinoin / Q000494:pharmacology*
chemical_list: D014212:Tretinoin; D002374:Catalase