title: Resveratrol attenuates doxorubicin-induced cellular damage by modulating nitric oxide and apoptosis.
abstract: Although doxorubicin (DOX) is a commonly used chemotherapeutic agent its clinical use is restricted due to its organ toxicities. The present investigation relates to reducing DOX induced side effects to the liver, kidney and ileum by usage of the antioxidant, anti-inflammatory agent, resveratrol (RES) and to investigate the role of nitric oxide synthase (NOS) in the process. Wistar rats were divided into four groups: control (saline i.p); DOX (20 mg/kg i.p), RES (20 mg/kg i.p) and DOX (20mg/kg i.p)+RES (20 mg/kg i.p). Immunohistochemical activity of both iNOS and eNOS were evaluated after DOX treatment and ultrastructural changes such as cellular damage and mitochondrial degeneration were evaluated. Degenerative ultrastructural changes were demonstrated especially in the DOX treated group. Variations in biochemical marker levels of oxidative stress on ischemia in tissues were not observed. Our data indicate that RES may prevent cellular damage in the early phase of DOX induced toxicity. RES could be used with its beneficial effects during early cellular damage in organ toxicity after DOX treatment in cancer patients.
authors.affiliation: Ege University Medical Faculty, Department of Histology and Embryology, TR-35100 Izmir, Turkey. gulperi.oktem@ege.edu.tr
authors.forename: Gulperi
authors.initials: G
authors.lastname: Oktem
authors.forename: Aysegul
authors.initials: A
authors.lastname: Uysal
authors.forename: Onur
authors.initials: O
authors.lastname: Oral
authors.forename: Ebru Demirel
authors.initials: ED
authors.lastname: Sezer
authors.forename: Murat
authors.initials: M
authors.lastname: Olukman
authors.forename: Ayşe
authors.initials: A
authors.lastname: Erol
authors.forename: Serap A
authors.initials: SA
authors.lastname: Akgur
authors.forename: Ayhan
authors.initials: A
authors.lastname: Bilir
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000633:toxicity*; D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D004317:Doxorubicin / Q000633:toxicity*; D007082:Ileum / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D007150:Immunohistochemistry; D007668:Kidney / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D008297:Male; D046529:Microscopy, Electron, Transmission; D009569:Nitric Oxide / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000032:analysis / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D000975:Antioxidants; D013267:Stilbenes; D009569:Nitric Oxide; D004317:Doxorubicin; D019001:Nitric Oxide Synthase; D000077185:Resveratrol