title: Lymphatic absorption of choline plasmalogen is much higher than that of ethanolamine plasmalogen in rats.
abstract: BACKGROUND
  Plasmalogen is a subclass of phospholipids widely distributed in animal tissues and ingested as food; however, the absorptive characteristics of different classes of plasmalogen have not been clarified.
  
  
  OBJECTIVE
  Our object was to compare the lymphatic output of choline and ethanolamine plasmalogens after an administration of phospholipid preparations containing each class of plasmalogens, and to analyze molecular species of plasmalogen absorbed into the lymph.
  
  
  METHODS
  A duodenal infusion of 1 ml of 10% emulsion of choline phospholipid (PC) containing 50.6% choline plasmalogen (PlsCho) or ethanolamine phospholipid (PE) containing 52.5% ethanolamine plasmalogen (PlsEtn) was administered in the lymph duct-cannulated rats. Molecular species of plasmalogen absorbed into the lymph were measured by LC-MS/MS.
  
  
  RESULTS
  Lymph outputs of PlsCho and PlsEtn increased and reached a peak value at 3 h after PC and PE injection, respectively. The peak value of PlsCho was much higher and remained at a high level until 8 h, whereas PlsEtn output fell to half of the peak value at 7 h. Total lymphatic output of PlsCho was 5-times higher than that of PlsEtn. Compositions of sn-1 in lymph plasmalogens roughly reflected those of the injected lipids, whereas sn-2 in both PlsCho and PlsEtn was rich in arachidonic acid (20:4) regardless of the composition of the administered fatty acid. Both plasmalogen and lysoplasmalogen after PE injection were not released into the portal vein.
  
  
  CONCLUSIONS
  Lymphatic absorption of PlsCho is much higher than that of PlsEtn in rats, and plasmalogens are re-esterified as 20:4-rich forms in the small intestine.
authors.affiliation: Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University, Kita-9, Nishi-9, Kita-ku, Sapporo, Hokkaido 060-8589, Japan.
authors.forename: Megumi
authors.initials: M
authors.lastname: Nishimukai
authors.forename: Maya
authors.initials: M
authors.lastname: Yamashita
authors.forename: Yudai
authors.initials: Y
authors.lastname: Watanabe
authors.forename: Yuya
authors.initials: Y
authors.lastname: Yamazaki
authors.forename: Toru
authors.initials: T
authors.lastname: Nezu
authors.forename: Ryouta
authors.initials: R
authors.lastname: Maeba
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hara
mesh_terms: D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D004655:Emulsions; D004951:Esterification; D007408:Intestinal Absorption; D007536:Isomerism; D007700:Kinetics; D008196:Lymph / Q000378:metabolism*; D008208:Lymphatic System / Q000502:physiology; D008246:Lysophospholipids / Q000097:blood / Q000737:chemistry / Q000378:metabolism; D008297:Male; D010955:Plasmalogens / Q000097:blood / Q000737:chemistry / Q000378:metabolism*; D011169:Portal Vein; D051381:Rats; D017208:Rats, Wistar; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
chemical_list: D004655:Emulsions; D008246:Lysophospholipids; D010955:Plasmalogens; C032459:choline plasmalogens; C021215:lysoplasmalogens; C020791:phosphatidal ethanolamines; D016718:Arachidonic Acid