title: [Effect of antisense oligonucleotides of ryanodine receptor on proliferation and [Ca2+]i of airway smooth muscle cells].
abstract: OBJECTIVE
  To investigate the effect of antisense oligonucleotides (ASON) of ryanodine receptor on proliferation and [Ca2+]i concentration of airway smooth muscle cells (ASMCs).
  
  
  METHODS
  ASMCs were cultivated with collagen enzyme digestion method. Different concentrations of ASON were added to the cultures with Lipofectamine 2000 to observe the ASMCs proliferation using MTS/PES method. The changes of ASMCs [Ca2+]i were also observed by flow cytometry. The expression of mRNA of subtypes of RyR was assayed by RT-PCR.
  
  
  RESULTS
  RyR ASON restrained the proliferation of ASMCs, decreased the expression of RyR and reduced the concentration of [Ca2+]i.
  
  
  CONCLUSIONS
  RyR ASON could inhibit the proliferation of ASMCs by influencing the concentration of [Ca2+]i after excited.
authors.affiliation: Department of Respiration, Second Artilleryforces, General Hospital of PLA, Beijing 100088, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.forename: Sui-Yang
authors.initials: SY
authors.lastname: Zhang
authors.forename: Gui-Sheng
authors.initials: GS
authors.lastname: Qian
authors.forename: Xi-Liang
authors.initials: XL
authors.lastname: Wang
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels; D002455:Cell Division; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D051381:Rats; D012137:Respiratory System; D019837:Ryanodine Receptor Calcium Release Channel / Q000235:genetics / Q000494:pharmacology*
chemical_list: D015220:Calcium Channels; D016376:Oligonucleotides, Antisense; D019837:Ryanodine Receptor Calcium Release Channel; D002118:Calcium