title: Chromatin condensation via the condensin II complex is required for peripheral T-cell quiescence.
abstract: Naive T cells encountering their cognate antigen become activated and acquire the ability to proliferate in response to cytokines. Stat5 is an essential component in this response. We demonstrate that Stat5 cannot access DNA in naive T cells and acquires this ability only after T-cell receptor (TCR) engagement. The transition is not associated with changes in DNA methylation or global histone modification but rather chromatin decondensation. Condensation occurs during thymocyte development and proper condensation is dependent on kleisin-β of the condensin II complex. Our findings suggest that this unique chromatin condensation, which can affect interpretations of chromatin accessibility assays, is required for proper T-cell development and maintenance of the quiescent state. This mechanism ensures that cytokine driven proliferation can only occur in the context of TCR stimulation.
authors.affiliation: Department of Biochemistry, St Jude Children's Research Hospital, Memphis, TN, USA.
authors.forename: Jason S
authors.initials: JS
authors.lastname: Rawlings
authors.forename: Martina
authors.initials: M
authors.lastname: Gatzka
authors.forename: Paul G
authors.initials: PG
authors.lastname: Thomas
authors.forename: James N
authors.initials: JN
authors.lastname: Ihle
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D049109:Cell Proliferation; D042002:Chromatin Assembly and Disassembly / Q000276:immunology* / Q000502:physiology; D047369:Chromatin Immunoprecipitation; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation / Q000276:immunology*; D007111:Immunity, Cellular / Q000276:immunology*; D007376:Interleukin-2 / Q000378:metabolism; D008213:Lymphocyte Activation / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D046912:Multiprotein Complexes / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000235:genetics; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism; D050799:STAT5 Transcription Factor / Q000235:genetics / Q000276:immunology*; D017422:Sequence Analysis, DNA; D016176:T-Lymphocyte Subsets / Q000166:cytology / Q000276:immunology
chemical_list: D004268:DNA-Binding Proteins; D007376:Interleukin-2; D046912:Multiprotein Complexes; D011948:Receptors, Antigen, T-Cell; D050799:STAT5 Transcription Factor; C108312:condensin complexes; D000251:Adenosine Triphosphatases