title: Expression of T-bet and GATA-3 in peripheral blood mononuclear cells of patients with oral lichen planus.
abstract: OBJECTIVE
  Oral lichen planus (OLP) is a T-cell-mediated chronic inflammatory oral disease of unknown aetiology. Imbalanced cytokine production by Th1 and Th2 probably contributes to the pathogenesis of OLP. Growing evidence has suggested that two Th1/Th2-specific transcription factors, T-bet and GATA-3, may play a critical role in the development of Th1 and Th2 immunity. The aim of the present study was to investigate the mRNA expressions of T-bet and GATA-3 in the peripheral blood mononuclear cells of OLP subjects, and their expression patterns in relation to several clinical features.
  
  
  METHODS
  Expressions of T-bet and GATA-3 mRNA in peripheral blood mononuclear cells isolated from twenty-eight OLP subjects and sixteen controls were detected by real-time reverse transcription-polymerase chain reaction.
  
  
  RESULTS
  When OLP subjects were regarded as a whole group, T-bet mRNA level and the ratio of T-bet/GATA-3 mRNA in OLP subjects were significantly higher (P<0.05) than those in controls. When the OLP subjects were divided according to different clinical forms, genders or age groups, T-bet, but not GATA-3, mRNA levels in reticular (P<0.01), female (P<0.05) and elder (age>55, P<0.05) OLP patients were significantly higher than those in control subjects. T-bet/GATA-3 mRNA ratio only in reticular (P<0.05) OLP subjects was significantly higher than that in control subjects.
  
  
  CONCLUSIONS
  The results implicate a predominant role of Th1-type immune response in pathogenesis of OLP. Different gene expressions of T-bet in different clinical features may indicate different immunoregulatory mechanisms of OLP.
authors.affiliation: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST), & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology,Wuhan University, PR China
authors.forename: Rui
authors.initials: R
authors.lastname: Lu
authors.forename: Gang
authors.initials: G
authors.lastname: Zhou
authors.forename: Gefei
authors.initials: G
authors.lastname: Du
authors.forename: Xueyi
authors.initials: X
authors.lastname: Xu
authors.forename: Jianguang
authors.initials: J
authors.lastname: Yang
authors.forename: Jingyu
authors.initials: J
authors.lastname: Hu
mesh_terms: D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D005260:Female; D050990:GATA3 Transcription Factor / Q000097:blood*; D015870:Gene Expression; D006801:Humans; D017676:Lichen Planus, Oral / Q000097:blood*; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000097:blood; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018709:Statistics, Nonparametric; D020825:T-Box Domain Proteins / Q000097:blood*; D000098815:T-bet Transcription Factor
chemical_list: D050990:GATA3 Transcription Factor; D012333:RNA, Messenger; D020825:T-Box Domain Proteins; D000098815:T-bet Transcription Factor