title: Cancer chemopreventive potential of luteolin-7-O-glucoside isolated from Ophiorrhiza mungos Linn.
abstract: The anticarcinogenic potential of the phytocompound Luteolin-7-O-Glucoside (LUT7G), isolated from the leaves of Ophiorrhiza mungos Linn, was studied against 4 different cancer cell lines (COLO 320 DM, AGS, MCF-7, and A549) and normal VERO cell line. The ability of LUT7G to induce apoptosis was determined by its antiradical activity, DNA fragmentation, expression of β-catenin, and chemopreventive efficacy in vivo by administering rats with DMH (20 mg/kg b.w., s.c.) for 4 consecutive wk and supplementing with 3 different doses throughout the experimental period of 16 wk. LUT7G scavenged 80% of DPPH radicals generated in vitro at 1000 μM and suppressed the expression of β-catenin to 40% at 120 μM concentrations. LUT7G induced apoptosis by scavenging ROS and suppressing the expression of β-catenin in COLO 320 DM cells and effectively inhibited ACF development in DMH-induced experimental carcinogenesis. Hence LUT7G can be a potent anticancer drug for colon carcinogenesis.
authors.affiliation: Department of Community Health Sciences, College of Applied Medical Sciences, King Saud University, Riyadh, Kingdom of Saudi Arabia.
authors.forename: Arul Albert
authors.initials: AA
authors.lastname: Baskar
authors.forename: Savarimuthu
authors.initials: S
authors.lastname: Ignacimuthu
authors.forename: Gabriel Paulraj
authors.initials: GP
authors.lastname: Michael
authors.forename: Khalid S
authors.initials: KS
authors.lastname: Al Numair
mesh_terms: D019813:1,2-Dimethylhydrazine; D058739:Aberrant Crypt Foci / Q000517:prevention & control; D000818:Animals; D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D047309:Flavones / Q000494:pharmacology*; D005960:Glucosides / Q000494:pharmacology*; D006801:Humans; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D019666:Rubiaceae / Q000737:chemistry*; D051176:beta Catenin / Q000235:genetics; D047311:Luteolin
chemical_list: D019813:1,2-Dimethylhydrazine; D016588:Anticarcinogenic Agents; D000975:Antioxidants; D047309:Flavones; D005960:Glucosides; D017382:Reactive Oxygen Species; D051176:beta Catenin; C066408:luteolin-7-glucoside; D047311:Luteolin