title: Changes in pituitary and prolactin cells of Wistar rats after two dental fillings with bisphenolic resins.
abstract: Bisphenol-A (BPA) is used to manufacture dental materials such as sealants, fillings and cements. There is evidence of its estrogenic effects on recipients after the placement of dental sealants. Pituitary and especially prolactin (PRL) cells are targets for estrogens.
  
  
  OBJECTIVE
  The aim of this research was to determine if BPA eluted from dental resins can alter the proliferation of pituitary cells and PRL cells in the short, medium and long term in a case-control assay.
  
  
  METHODS
  Two dental fillings were inserted in the lower incisors of Wistar rats divided into groups sacrificed after one, three, five and seven months. Immunocytochemical treatment was carried out in order to determine proliferating cell nuclear antigen (PCNA) positive cells, PRL-positive cells, PRL- and PCNA-positive cells.
  
  
  RESULTS
  A significant increase of PCNA-positive cells after one (p < 0.05), three (p < 0.01) and five months (p < 0.01) was recorded. PRL-positive cells showed no statistically significant difference between intervened animals and controls. PRL- and PCNA-positive cells manifested a significant increase after five months (p < 0.05). A significant decrease in proliferating cells was observed after seven months (p < 0.05) for PCNA-positive cells and (p < 0.01) for PRL- and PCNA-positive cells.
  
  
  CONCLUSIONS
  Low quantities of BPA eluted during mastication can affect immunocytochemical patterns of pituitary cells, increasing cellular proliferation in the short, medium and long term although PRL cell population remained unaffected after dental fillings.
authors.affiliation: Department of Surgery, University of Salamanca, Spain. evelasco@usal.es
authors.forename: Eva
authors.initials: E
authors.lastname: Velasco-Marinero
authors.forename: Julio J
authors.initials: JJ
authors.lastname: Herrero-Payo
authors.forename: José
authors.initials: J
authors.lastname: Carretero-González
mesh_terms: D000818:Animals; D001559:Benzhydryl Compounds; D049109:Cell Proliferation / Q000187:drug effects; D003764:Dental Materials / Q000494:pharmacology*; D003793:Dental Restoration, Permanent / Q000295:instrumentation*; D007150:Immunohistochemistry; D008297:Male; D010636:Phenols / Q000494:pharmacology*; D010902:Pituitary Gland / Q000166:cytology*; D011388:Prolactin / Q000378:metabolism*; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D003764:Dental Materials; D010636:Phenols; D011388:Prolactin; D018809:Proliferating Cell Nuclear Antigen; C006780:bisphenol A; D000098826:Bisphenol A Compounds