title: [Cloning and sequencing of alpha, beta globin coding genes in Tibetans living at high altitude].
abstract: OBJECTIVE
  To explore the molecular biological mechanism of hemoglobin with high oxygen affinity in Tibetans by determining the sequence of globin cDNA in Tibetans living at high altitude.
  
  
  METHODS
  Total RNA was isolated from human bone marrow samples of three Tibetans who live in Qinghai-Tibet plateau. cDNA fragments coding for alpha and beta genes of human hemoglobin were obtained through RT-PCR and were ligated to plasmid pGEM-T easy vectors, and then the ligation liquid were transformed to Escherichia coli and cloned and sequenced. Nucleotide sequences were compared with GenBank data by BLAST method.
  
  
  RESULTS
  sequence of a globin cDNA in Tibetans were the same with the registering globin genes in the GenBank, and Hb Abruzzo (beta143 (H21), His- > Arg) gene mutation, a high oxygen affinity beta globin mutation, was found in one Tibetan' beta goblin coding gene (CAC- > CGC).
  
  
  CONCLUSIONS
  This hemoglobin gene mutation may be associated with high altitude adaptation of Tibetans living at high altitude.
authors.affiliation: Institute of Health and Environmental Medicine, Tianjin 300050, China.
authors.forename: Hong-bin
authors.initials: HB
authors.lastname: Dong
authors.forename: Xin
authors.initials: X
authors.lastname: Hong
authors.forename: Hong-jing
authors.initials: HJ
authors.lastname: Nie
authors.forename: Zhong-hai
authors.initials: ZH
authors.lastname: Xiao
authors.forename: Gui-xiang
authors.initials: GX
authors.lastname: He
authors.forename: Shang-han
authors.initials: SH
authors.lastname: Hei
authors.forename: Zhao-yun
authors.initials: ZY
authors.lastname: Yin
mesh_terms: D000328:Adult; D000531:Altitude; D044466:Asian People / Q000235:genetics; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D006455:Hemoglobins, Abnormal / Q000235:genetics; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D012313:RNA; D017422:Sequence Analysis, DNA; D018609:Tibet; D055542:alpha-Globins / Q000235:genetics*; D055544:beta-Globins / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D006455:Hemoglobins, Abnormal; D055542:alpha-Globins; D055544:beta-Globins; C067281:hemoglobin Abruzzo; D012313:RNA