title: Cardiac differentiation promotes mitochondria development and ameliorates oxidative capacity in H9c2 cardiomyoblasts.
abstract: H9c2 undergoing cardiac differentiation induced by all-trans-retinoic acid were investigated for mitochondria structural features together with the implied functional changes, as a model for the study of mitochondrial development in cardiogenic progenitor cells. As the expression of cardiac markers became detectable, mitochondrial mass increased and mitochondrial morphology and ultrastructure changed. Reticular network organization developed and more bulky mitochondria with greater numbers of closely packed cristae and more electron-dense matrix were detected. Increased expression of PGC-1α proved the occurrence of mitochondrial biogenesis. Improvements in mitochondrial energetic competence were also documented, linked to better assembly between F(0) and F(1) sectors of the F(0)F(1)ATPsynthase enzyme complex.
authors.affiliation: Department of Biomedical Sciences and Technologies, University of Udine, Udine, Italy.
authors.forename: Marina
authors.initials: M
authors.lastname: Comelli
authors.forename: Rossana
authors.initials: R
authors.lastname: Domenis
authors.forename: Elena
authors.initials: E
authors.lastname: Bisetto
authors.forename: Magali
authors.initials: M
authors.lastname: Contin
authors.forename: Maurizio
authors.initials: M
authors.lastname: Marchini
authors.forename: Fulvia
authors.initials: F
authors.lastname: Ortolani
authors.forename: Lara
authors.initials: L
authors.lastname: Tomasetig
authors.forename: Irene
authors.initials: I
authors.lastname: Mavelli
mesh_terms: D002454:Cell Differentiation; D002460:Cell Line; D005434:Flow Cytometry; D006801:Humans; D018613:Microscopy, Confocal; D046529:Microscopy, Electron, Transmission; D008929:Mitochondria, Heart / Q000378:metabolism*; D009206:Myocardium / Q000166:cytology*; D010084:Oxidation-Reduction