title: Correlating morphometric parameters of the porcine optic nerve head in spectral domain optical coherence tomography with histological sections.
abstract: Aim To correlate morphometric parameters of the porcine optic nerve head (ONH) in spectral domain optical coherence tomography (SD-OCT) B-scans with matched histological sections. Methods Ten porcine eyes were scanned using the Spectralis SD-OCT (Heidelberg Engineering, Heidelberg, Germany) at 7 mm Hg and subsequently fixed, cryosectioned and stained with Van Giessen stain. B-scans were matched with histological sections and morphometric parameters measured on both. Results SD-OCT measurements of the Bruch membrane opening (BMO) cross-sectional diameter and the mean distance from the BMO plane to the anterior surface of the collagenous lamina cribrosa correlated strongly with matched histological measurements (r=0.80 (p=0.005) and 0.83 (p=0.003), respectively) while measurements of the mean prelaminar tissue thickness, cup cross-sectional area and neuroretinal rim cross-sectional area had lower correlation coefficients (r=0.62 (p=0.055), 0.57 (p=0.08) and 0.33 (p=0.354), respectively). SD-OCT measurement of lamina cribrosa mean cross-sectional thickness also showed a lower correlation with matched histological measurements (r=0.64, p=0.048). Conclusion A high correlation exists between SD-OCT B-scans and matched histological sections for anterior collagenous structures of the ONH, while a lower correlation exists for morphometric parameters involving the prelaminar region and lamina cribrosa thickness.
authors.affiliation: Centre for Ophthalmology and Visual Science, The University of Western Australia, Nedlands, WA, 6009, Australia.
authors.forename: Naeem
authors.initials: N
authors.lastname: Fatehee
authors.forename: Paula K
authors.initials: PK
authors.lastname: Yu
authors.forename: William H
authors.initials: WH
authors.lastname: Morgan
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Cringle
authors.forename: Dao-Yi
authors.initials: DY
authors.lastname: Yu
mesh_terms: D019412:Anatomy, Cross-Sectional; D000818:Animals; D001699:Biometry; D007429:Intraocular Pressure / Q000502:physiology; D009412:Nerve Fibers; D009898:Optic Disk / Q000033:anatomy & histology*; D034421:Sus scrofa; D041623:Tomography, Optical Coherence / Q000379:methods*