title: IS6110-restriction fragment length polymorphism and spoligotyping analysis of Mycobacterium tuberculosis clinical isolates for investigating epidemiologic distribution in Korea.
abstract: The Beijing family of Mycobacterium tuberculosis has been emerging in the world. However, there are few nationwide data of genotypic distribution in Korea. This study aimed to identify the genotypic diversity of clinical isolates of M. tuberculosis and to demonstrate the population of Beijing family in Korea. We collected 96 clinical M. tuberculosis isolates from 11 university hospitals nationwide in Korea from 2008 to 2009. We observed 24 clusters in IS6110-RFLP analysis and 19 patterns in spoligotyping. Seventy-five isolates were confirmed to be Beijing family. Two isolates of the K strain and 12 isolates of the K family strain were also found. We found that drug resistance phenotypes were more strongly associated with Beijing family than non-Beijing family (P=0.003). This study gives an overview of the distribution of genotypes of M. tuberculosis in Korea. These findings indicate that we have to pay more attention to control of M. tuberculosis strains associated with the Beijing family.
authors.affiliation: Department of Laboratory Medicine, School of Medicine, Pusan National University, Busan, Korea.
authors.forename: Go Eun
authors.initials: GE
authors.lastname: Choi
authors.forename: Mi Hee
authors.initials: MH
authors.lastname: Jang
authors.forename: Eun Ju
authors.initials: EJ
authors.lastname: Song
authors.forename: Seok Hoon
authors.initials: SH
authors.lastname: Jeong
authors.forename: Jae-Seok
authors.initials: JS
authors.lastname: Kim
authors.forename: Wee Gyo
authors.initials: WG
authors.lastname: Lee
authors.forename: Young
authors.initials: Y
authors.lastname: Uh
authors.forename: Kyoung Ho
authors.initials: KH
authors.lastname: Roh
authors.forename: Hye Soo
authors.initials: HS
authors.lastname: Lee
authors.forename: Jong Hee
authors.initials: JH
authors.lastname: Shin
authors.forename: Nam Hee
authors.initials: NH
authors.lastname: Ryoo
authors.forename: Young Ree
authors.initials: YR
authors.lastname: Kim
authors.forename: Joseph
authors.initials: J
authors.lastname: Jeong
authors.forename: Jee Hee
authors.initials: JH
authors.lastname: Kim
authors.forename: Sun Min
authors.initials: SM
authors.lastname: Lee
authors.forename: Jongyoun
authors.initials: J
authors.lastname: Yi
authors.forename: Sang Hyun
authors.initials: SH
authors.lastname: Hwang
authors.forename: Hyung Hoi
authors.initials: HH
authors.lastname: Kim
authors.forename: Eun Yup
authors.initials: EY
authors.lastname: Lee
authors.forename: Chulhun L
authors.initials: CL
authors.lastname: Chang
authors.forename: Moon-Bum
authors.initials: MB
authors.lastname: Kim
authors.forename: Yeong Dae
authors.initials: YD
authors.lastname: Kim
mesh_terms: D024881:Drug Resistance, Bacterial; D005838:Genotype; D006801:Humans; D008826:Microbial Sensitivity Tests; D009169:Mycobacterium tuberculosis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010641:Phenotype; D012150:Polymorphism, Restriction Fragment Length; D056910:Republic of Korea; D014376:Tuberculosis / Q000453:epidemiology* / Q000235:genetics / Q000382:microbiology