title: Modulation-frequency encoded multi-color fluorescent DNA analysis in an optofluidic chip.
abstract: We introduce a principle of parallel optical processing to an optofluidic lab-on-a-chip. During electrophoretic separation, the ultra-low limit of detection achieved with our set-up allows us to record fluorescence from covalently end-labeled DNA molecules. Different sets of exclusively color-labeled DNA fragments-otherwise rendered indistinguishable by spatio-temporal coincidence-are traced back to their origin by modulation-frequency-encoded multi-wavelength laser excitation, fluorescence detection with a single ultrasensitive, albeit color-blind photomultiplier, and Fourier analysis decoding. As a proof of principle, fragments obtained by multiplex ligation-dependent probe amplification from independent human genomic segments, associated with genetic predispositions to breast cancer and anemia, are simultaneously analyzed.
authors.affiliation: Integrated Optical MicroSystems, MESA+ Institute for Nanotecnology, University of Twente, Enschede, The Netherlands.
authors.forename: Chaitanya
authors.initials: C
authors.lastname: Dongre
authors.forename: Jasper
authors.initials: J
authors.lastname: van Weerd
authors.forename: Geert A J
authors.initials: GA
authors.lastname: Besselink
authors.forename: Rebeca Martinez
authors.initials: RM
authors.lastname: Vazquez
authors.forename: Roberto
authors.initials: R
authors.lastname: Osellame
authors.forename: Giulio
authors.initials: G
authors.lastname: Cerullo
authors.forename: Rob
authors.initials: R
authors.lastname: van Weeghel
authors.forename: Hans H
authors.initials: HH
authors.lastname: van den Vlekkert
authors.forename: Hugo J W M
authors.initials: HJ
authors.lastname: Hoekstra
authors.forename: Markus
authors.initials: M
authors.lastname: Pollnau
mesh_terms: D004247:DNA / Q000032:analysis*; D004586:Electrophoresis / Q000295:instrumentation / Q000379:methods; D005583:Fourier Analysis; D006801:Humans; D056656:Lab-On-A-Chip Devices; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation* / Q000379:methods; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence
chemical_list: D004247:DNA