title: [Effect of AcSDKP on the proliferation and collagen synthesis in cultured rat cardiac fibroblasts stimulated by PDGF].
abstract: OBJECTIVE
  To investigate whether AcSDKP can inhibit proliferation and collagen synthesis in cultured rat cardiac fibroblasts mediated by PDGF.
  
  
  METHODS
  Neonatal rat cardiac fibroblasts were isolated. The cell proliferation was observed by 3H-proline incorporation assay.
  
  
  RESULTS
  On the culture of 0.4% FBS, PDGF stimulated cardiac fibroblasts proliferation and collagen synthesis with a dose-dependent manner at the concentrations from 1 ng/ml to 20 ng/ml, in which 10 ng/ml PDGF reached its peak. AcSDKP at the concentration from 10(-10) mol/L to 10(-8) mol/L could inhibit cardiac fibroblasts proliferation and collagen synthesis mediated by PDGF. 10(-9) mol/L AcSDKP attained its peak on inhibiting cardiac fibroblasts proliferation and collagen synthesis.
  
  
  CONCLUSIONS
  AcSDKP can inhibit proliferation and collagen synthesis in cultured rat cardiac fibroblasts mediated by PDGF.
authors.affiliation: Experimental and Research Center, North China Coal Medical College, Tngshan 06300, China.
authors.forename: Xi-Ling
authors.initials: XL
authors.lastname: Zhu
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Wang
authors.forename: Fang
authors.initials: F
authors.lastname: Yang
authors.forename: Rui-Min
authors.initials: RM
authors.lastname: Wang
authors.forename: Wen-Dong
authors.initials: WD
authors.lastname: Ma
authors.forename: Ling
authors.initials: L
authors.lastname: Luo
authors.forename: Wan-Mi
authors.initials: WM
authors.lastname: Hu
authors.forename: Li-Juan
authors.initials: LJ
authors.lastname: Zhang
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D032386:Myoblasts, Cardiac / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D009842:Oligopeptides / Q000494:pharmacology*; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D009842:Oligopeptides; D010982:Platelet-Derived Growth Factor; D003094:Collagen; C058504:goralatide