title: Molecular characterization and expression analysis of ADAM12 during chicken embryonic development.
abstract: ADAM12 is a member of the disintegrin and metalloprotease (ADAM) family of molecules, which consist of multiple domains. ADAM12 is involved in different physiological and pathological processes. In the present study, full-length sequences of two chicken ADAM12 isoforms were cloned and identified by reverse transcription-polymerase chain reaction (RT-PCR), rapid amplification of cDNA ends methods and bioinformatics analysis. The long isoform consists of all domains characteristic for ADAMs and is strongly expressed in different tissues, whereas the short isoform lacks large parts of the metalloprotease and disintegrin domains and is only expressed weakly. Results from semi-quantitative RT-PCR show that the complete ADAM12 is stably expressed throughout chicken embryonic development, while the short isoform is only regionally detectable in the lung and brain. Results from in situ hybridization show that chicken ADAM12 is expressed exclusively in tissues and organs derived from the neural tube, the neural crest or the mesoderm, with a highly regulated spatiotemporal expression pattern. Our data confirm and extend studies of ADAM12 in other species, and suggest that ADAM12 may play a role in the development of several organs, including the formation of feather buds.
authors.affiliation: Institute of Anatomy I, University of Jena School of Medicine, Jena University Hospital, Teichgraben 7, D-07743 Jena, Germany.
authors.forename: Juntang
authors.initials: J
authors.lastname: Lin
authors.forename: Jiankai
authors.initials: J
authors.lastname: Luo
authors.forename: Christoph
authors.initials: C
authors.lastname: Redies
mesh_terms: D051722:ADAM Proteins / Q000235:genetics*; D000072199:ADAM12 Protein; D000818:Animals; D002642:Chick Embryo; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D017403:In Situ Hybridization; D008565:Membrane Proteins / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D008565:Membrane Proteins; D051722:ADAM Proteins; D000072199:ADAM12 Protein