title: Strategies for the rapid construction of conditionally-replicating HSV-1 vectors expressing foreign genes as anticancer therapeutic agents.
abstract: Conditionally replication-competent Herpes Simplex Virus Type 1 (HSV-1) vectors expressing foreign genes have been developed as experimental therapeutic agents. Traditional methods of virus construction, including growth selection based on thymidine kinase gene expression, and color selection based on a reporter gene expression are often time-consuming and relatively inefficient. This review summarizes the various strategies developed in recent years for the rapid and efficient construction of novel conditionally replication-competent mutant HSV expressing multiple foreign genes. Additionally, two new modifications of existing strategies, which have not been previously reported, are discussed.
authors.affiliation: Department of Pediatrics, Cell Biology and Anatomy, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA. jnparker@uab.edu
authors.forename: Jacqueline N
authors.initials: JN
authors.lastname: Parker
authors.forename: Xiaojia
authors.initials: X
authors.lastname: Zheng
authors.forename: William
authors.initials: W
authors.lastname: Luckett
authors.forename: James M
authors.initials: JM
authors.lastname: Markert
authors.forename: Kevin A
authors.initials: KA
authors.lastname: Cassady
mesh_terms: D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics*; D018259:Herpesvirus 1, Human / Q000235:genetics / Q000502:physiology*; D006801:Humans; D009369:Neoplasms / Q000235:genetics / Q000628:therapy*; D014779:Virus Replication / Q000235:genetics*