title: The effect of HTK solution modification by addition of prolactin on biochemical indices reflecting ischaemic damage to porcine kidney.
abstract: BACKGROUND
  The aim of our study was to evaluate the impact of perfusion with HTK solution, modified by the addition of prolactin (PRI), on selected biochemical parameters of porcine renal damage within 24 and 48 hours after the onset of cold ischemia time.
  
  
  METHODS
  Each study group consisted of 10 adult pigs. During harvesting the kidneys were rinsed with Ringer's solution (group 1), HTK (group 2), and HTK+PRL in a dose of 0.2 mg/dL, 0.02 mg/dL, and 0.01 mg/dL in groups 3, 4 and 5, respectively. The levels of lactate dehydrogenase, asparagine (AST) and alanine aminotransferases, lactates, total protein, potassium and calcium were determined in the perfusate. After 24 and 48 hours the rinsing procedure and the abovementioned tests were repeated.
  
  
  RESULTS
  After 24 hours of storage, in 4 groups, significantly lower levels of LDH (U/L) were recorded compared with HTK solution alone, namely 235 ± 93 versus 271 ± 125 (perfusion minute, 0), and 55 ± 21 versus 125 ± 94 (30th minute). Similar behavior pattern was presented by AST (U/L) and potassium (mmol/L), and the results were 31 ± 8 versus 35 ± 12 and 16 ± 10 versus 29 ± 14, and 12 ± 3 versus 16 ± 3 and 10 ± 1 versus 13 ± 1, respectively. The changes described above were not observed in the 48th hour of reperfusion.
  
  
  CONCLUSIONS
  Our study results indicate the possibility of cytoprotective action of PRL after adding it to the fluid perfusing kidneys during cold ischemia. This effect, observed after 24 hours of storage, was to a considerable extent dose dependent. In our experiment the effect was pronounced only at 0.02 mg/dL supply of PRL.
authors.affiliation: Department of General, Vascular and Transplant Surgery, Medical University of Silesia, Katowice, Poland. acaban@man.pl
authors.forename: A
authors.initials: A
authors.lastname: Caban
authors.forename: B
authors.initials: B
authors.lastname: Dolińska
authors.forename: G
authors.initials: G
authors.lastname: Oczkowicz
authors.forename: G
authors.initials: G
authors.lastname: Budziński
authors.forename: A
authors.initials: A
authors.lastname: Ostróżka-Cieślik
authors.forename: M
authors.initials: M
authors.lastname: Krzysztofik
authors.forename: L
authors.initials: L
authors.lastname: Cierpka
authors.forename: F
authors.initials: F
authors.lastname: Ryszka
mesh_terms: D000818:Animals; D005260:Female; D005947:Glucose; D007668:Kidney / Q000098:blood supply*; D008353:Mannitol; D011189:Potassium Chloride; D011343:Procaine; D011388:Prolactin / Q000008:administration & dosage*; D015427:Reperfusion Injury; D012996:Solutions; D013552:Swine
chemical_list: C039984:Bretschneider cardioplegic solution; D012996:Solutions; D008353:Mannitol; D011343:Procaine; D011189:Potassium Chloride; D011388:Prolactin; D005947:Glucose