title: [Effects of vagotomy on UCP2 mRNA expression and gastric acid secretion in rats].
abstract: OBJECTIVE
  The relationship between gastric acid secretion and ATP level, and regulation of uncoupling protein 2 (UCP2) mRNA expression by vagus nerve were studied in vagotomies rats.
  
  
  METHODS
  With the high selective vagotomy model, the gastric acidity was titrated to pH 7.0 with 0.1 mol/L NaOH solution and ATP contents were quantified by using fluorimetry. The expression of UCP2 mRNA was observed by using Northern blot in stomach of rats.
  
  
  RESULTS
  Both of gastric acidity and ATP contents in stomach body decreased significantly at 24 h after vagotomy. The expression of UCP2 mRNA was markedly increased as compared with sham operation group.
  
  
  CONCLUSIONS
  ATP contents decreased and vagus nerve down-regulates expression of UCP2 mRNA in stomach corpus in vagotomies rats. The results indicates that vagus nerve could underlay the gastric acidity by inhibiting expression of UCP2 mRNA and increasing ATP contents in rats.
authors.affiliation: Department of Physiology, Dalian Medical University, Dalian 116027, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zou
authors.forename: Mei
authors.initials: M
authors.lastname: Yang
authors.forename: De-Zheng
authors.initials: DZ
authors.lastname: Gong
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Guan
authors.forename: Nan
authors.initials: N
authors.lastname: Tian
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D005744:Gastric Acid / Q000378:metabolism*; D007473:Ion Channels / Q000235:genetics / Q000378:metabolism*; D008297:Male; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D000071246:Uncoupling Protein 2; D014628:Vagotomy; D014630:Vagus Nerve / Q000378:metabolism
chemical_list: D007473:Ion Channels; D024101:Mitochondrial Proteins; D012333:RNA, Messenger; C000604391:Ucp2 protein, rat; D000071246:Uncoupling Protein 2; D000255:Adenosine Triphosphate