title: Application and adaptation of the in vitro micronucleus assay for the assessment of nutritional requirements of cells for DNA damage prevention.
abstract: DNA damage is a fundamental cause of developmental and degenerative diseases. The in vitro cytokinesis-block micronucleus cytome (CBMN-Cyt) assay is an established comprehensive method for assessing cytostasis and chromosome stability in cells. Originally developed to study the acute effects of single environmental genotoxicants, creative applications and adaptations to the basic protocol have allowed its use in evaluating the impacts of dietary micronutrients and micronutrient combinations (nutriomes) on DNA damage. In this review, we examine some of these studies and the important findings they have generated with respect to nutrient/nutrient, nutrient/genotype and nutrient/genotoxicant interactions, as well as assessment of the carcinogenic (or protective) potential of whole dietary patterns. In addition, we outline current knowledge gaps and technical limitations and propose future adaptations to enhance the applicability of the CBMN-Cyt method for in vivo predictions.
authors.affiliation: CSIRO Food and Nutritional Sciences, Gate 13 Kintore Avenue, Adelaide, South Australia 5000, Australia.
authors.forename: Caroline F
authors.initials: CF
authors.lastname: Bull
authors.forename: Sasja
authors.initials: S
authors.lastname: Beetstra-Hill
authors.forename: Bianca J
authors.initials: BJ
authors.lastname: Benassi-Evans
authors.forename: Jimmy W
authors.initials: JW
authors.lastname: Crott
authors.forename: Michiyo
authors.initials: M
authors.lastname: Kimura
authors.forename: Theodora
authors.initials: T
authors.lastname: Teo
authors.forename: Jing
authors.initials: J
authors.lastname: Wu
authors.forename: Michael F
authors.initials: MF
authors.lastname: Fenech
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004249:DNA Damage / Q000187:drug effects*; D004032:Diet; D005502:Food; D005720:Gamma Rays; D006801:Humans; D048629:Micronuclei, Chromosome-Defective; D015162:Micronucleus Tests; D018977:Micronutrients / Q000008:administration & dosage*; D009751:Nutritional Requirements; D051381:Rats
chemical_list: D018977:Micronutrients