title: Inhibition of airway MUC5AC mucin production and gene expression induced by epidermal growth factor or phorbol ester by glycyrrhizin and carbenoxolone.
abstract: OBJECTIVE
  In this study, we investigated whether glycyrrhizin and carbenoxolone affect MUC5AC mucin production and gene expression induced by epidermal growth factor (EGF) or phorbol ester (PMA) from human airway epithelial cells.
  
  
  METHODS
  Confluent NCI-H292 cells were pretreated with each agent for 30 min and then stimulated with EGF and PMA for 24h, respectively. MUC5AC mucin gene expression and mucin protein production were measured by RT-PCR and ELISA.
  
  
  RESULTS
  Glycyrrhizin and carbenoxolone were found to inhibit the production of MUC5AC mucin protein induced by EGF or PMA, and both compounds also inhibited the expression of MUC5AC mucin gene induced by EGF or PMA.
  
  
  CONCLUSIONS
  These results suggest that glycyrrhizin and carbenoxolone can inhibit mucin gene expression and production of mucin protein, by directly acting on airway epithelial cells.
authors.affiliation: Department of Pharmacology, School of Medicine, Chungnam National University, Joong-Gu, Daejeon, Republic of Korea.
authors.forename: Hyun Jae
authors.initials: HJ
authors.lastname: Lee
authors.forename: Su Yel
authors.initials: SY
authors.lastname: Lee
authors.forename: Heung Seog
authors.initials: HS
authors.lastname: Bae
authors.forename: Jang-Hyun
authors.initials: JH
authors.lastname: Kim
authors.forename: Gyu Tae
authors.initials: GT
authors.lastname: Chang
authors.forename: Jeong Ho
authors.initials: JH
authors.lastname: Seok
authors.forename: Choong Jae
authors.initials: CJ
authors.lastname: Lee
mesh_terms: D002229:Carbenoxolone / Q000494:pharmacology*; D045744:Cell Line, Tumor; D004347:Drug Interactions; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D019695:Glycyrrhizic Acid / Q000494:pharmacology*; D006801:Humans; D055271:Mucin 5AC / Q000037:antagonists & inhibitors* / Q000096:biosynthesis / Q000235:genetics; D010936:Plant Extracts / Q000494:pharmacology*; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D020545:Respiratory Mucosa / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
chemical_list: D055271:Mucin 5AC; D010936:Plant Extracts; D011500:Protein Synthesis Inhibitors; D004815:Epidermal Growth Factor; D019695:Glycyrrhizic Acid; D002229:Carbenoxolone; D013755:Tetradecanoylphorbol Acetate