title: Genomic sequences comparison and differential expression of miiuy croaker MHC class I gene, after infection by Vibrio anguillarum.
abstract: Major histocompatibility complex (MHC) has a central role in the adaptive immune system by presenting foreign peptide to the T-cell receptor. MHC gene family contains two main subgroups of immunologically active molecules. In order to study the molecular function and genomic characteristic of class I gene in teleost, the full lengths of MHC class Iα cDNA and genomic sequence were cloned from miiuy croaker (Miichthys miiuy). Seven exons and six introns were identified in miiuy croaker class Iα gene. This genomic structural feature of miiuy croaker is similar to that present in some fishes such as Japanese flounder and Atlantic salmon, but different from that present in some other fishes such as half-smooth tongue sole and channel catfish. The deduced amino acid sequence of class Iα gene had 25.9-54.1% identity with those of mammal and teleost. Real-time quantitative RT-PCR demonstrated that the MHC class Iα gene was ubiquitously expressed in 10 normal tissues; expression levels of MHC Iα gene were found first upregulated and then downregulated throughout the pathogenic bacteria infection process in spleen and kidney.
authors.affiliation: Key Laboratory for Marine Living Resources and Molecular Engineering, College of Marine Science, Zhejiang Ocean University, Zhejiang Province, Zhoushan 316000, PR China.
authors.forename: Tian-jun
authors.initials: TJ
authors.lastname: Xu
authors.forename: Yue-na
authors.initials: YN
authors.lastname: Sun
authors.forename: Yuan-zhi
authors.initials: YZ
authors.lastname: Cheng
authors.forename: Ge
authors.initials: G
authors.lastname: Shi
authors.forename: Ri-xin
authors.initials: RX
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005786:Gene Expression Regulation; D005805:Genes, MHC Class I; D010473:Perciformes / Q000235:genetics* / Q000276:immunology* / Q000821:virology; D014733:Vibrio; D014735:Vibrio Infections / Q000235:genetics / Q000276:immunology / Q000662:veterinary*