title: Brain extracellular fluid protein changes in acute stroke patients.
abstract: In vivo human brain extracellular fluids (ECF) of acute stroke patients were investigated to assess the changes in protein levels associated with ischemic damages. Microdialysates (MDs) from the infarct core (IC), the penumbra (P), and the unaffected contralateral (CT) brain regions of patients suffering an ischemic stroke (n = 6) were compared using a shotgun proteomic approach based on isobaric tagging and mass spectrometry. Quantitative analysis showed 53 proteins with increased amounts in the IC or P with respect to the CT samples. Glutathione S-transferase P (GSTP1), peroxiredoxin-1 (PRDX1), and protein S100-B (S100B) were further assessed with ELISA on the blood of unrelated control (n = 14) and stroke (n = 14) patients. Significant increases of 8- (p = 0.0002), 20- (p = 0.0001), and 11-fold (p = 0.0093) were found, respectively. This study highlights the value of ECF as an efficient source to further discover blood stroke markers.
authors.affiliation: Biomedical Proteomics Group, Department of Structural Biology and Bioinformatics, Faculty of Medicine, University of Geneva, Geneva, Switzerland.
authors.forename: Loï
authors.initials: L
authors.lastname: Dayon
authors.forename: Natacha
authors.initials: N
authors.lastname: Turck
authors.forename: Teresa
authors.initials: T
authors.lastname: Garcí-Berrocoso
authors.forename: Nadia
authors.initials: N
authors.lastname: Walter
authors.forename: Pierre R
authors.initials: PR
authors.lastname: Burkhard
authors.forename: Anna
authors.initials: A
authors.lastname: Vilalta
authors.forename: Juan
authors.initials: J
authors.lastname: Sahuquillo
authors.forename: Joan
authors.initials: J
authors.lastname: Montaner
authors.forename: Jean-Charles
authors.initials: JC
authors.lastname: Sanchez
mesh_terms: D000328:Adult; D000818:Animals; D015415:Biomarkers / Q000032:analysis*; D001921:Brain / Q000473:pathology*; D001923:Brain Chemistry; D045604:Extracellular Fluid / Q000737:chemistry*; D005260:Female; D006801:Humans; D007118:Immunoassay / Q000379:methods; D008297:Male; D017551:Microdialysis; D008875:Middle Aged; D011506:Proteins / Q000032:analysis*; D020521:Stroke / Q000378:metabolism* / Q000473:pathology
chemical_list: D015415:Biomarkers; D011506:Proteins