title: Ionizing radiation-induced DNA strand breaks and γ-H2AXγ-H2AX foci in cells exposed to nitric oxide.
abstract: A number of studies have demonstrated that nitric oxide enhances radiosensitivity of anoxic and hypoxic cells in vitro and in vivo, and some evidence points to a role for DNA damage and repair in this phenomenon. We have recently observed that nitric oxide enhances the formation of DNA single- and double-strand breaks following ionising irradiation, measured by the alkaline comet assay and immunofluorescence microscopy for γ-H2AXγ-H2AX.
authors.affiliation: Health Protection Agency Centre for Radiation, Chemical & Environmental Hazards, 0X11 0RQ, Chilton, Oxon, UK. kai.rothkamm@hpa.org.uk
authors.forename: Kai
authors.initials: K
authors.lastname: Rothkamm
authors.forename: Susanne
authors.initials: S
authors.lastname: Burdak-Rothkamm
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D020552:Comet Assay; D006224:Cricetinae; D003412:Cricetulus; D053960:DNA Breaks / Q000187:drug effects* / Q000528:radiation effects*; D006657:Histones / Q000378:metabolism*; D006801:Humans; D008856:Microscopy, Fluorescence; D009569:Nitric Oxide / Q000494:pharmacology*; D011839:Radiation, Ionizing
chemical_list: C468783:H2AX protein, human; D006657:Histones; D009569:Nitric Oxide