title: Down-regulation of Prdx6 contributes to DNA vaccine induced vitiligo in mice.
abstract: DNA vaccines are widely used against infectious agents for their ability to induce both humoral and cellular immune responses. However, safety concerns regarding autoimmune responses to DNA vaccines, particularly to certain plasmids, should not be neglected. In this study, we serendipitously found that mice inoculated with pcDNA3-ANXB1 (pcDNA3-b1) developed autoimmunity, which did not happen in pVAX-ANXB1 (pVAX-b1) inoculated mice. We also employed proteomics approaches to investigate the distinction between the two groups of DNA vaccine immunized mice. Five different proteins with three-fold or greater changes were separated and identified by two-dimensional electrophoresis. Our study verified the safety of the DNA vaccine and unveiled the underlying potential molecular mechanism of DNA vaccine delivery.
authors.affiliation: Department of Medical Genetics, Second Military Medical University, 800 Xiang-Yin Road, Shanghai 200433, P. R. China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhou
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Fu
authors.initials: F
authors.lastname: Yang
authors.forename: Yue
authors.initials: Y
authors.lastname: Wang
authors.forename: Shuhan
authors.initials: S
authors.lastname: Sun
mesh_terms: D000818:Animals; D015551:Autoimmunity; D015536:Down-Regulation; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D054465:Peroxiredoxin VI / Q000032:analysis / Q000378:metabolism*; D040901:Proteomics; D019444:Vaccines, DNA / Q000009:adverse effects* / Q000276:immunology; D014820:Vitiligo / Q000209:etiology* / Q000276:immunology
chemical_list: D019444:Vaccines, DNA; D054465:Peroxiredoxin VI; C515983:Prdx6 protein, mouse