title: [Neural pathway participates in protection of limb ischemic preconditioning against brain injuries induced by ischemia/reperfusion in rats].
abstract: OBJECTIVE
  To explore the role of femoral nerves section (FNS) on the protection of limb ischemic preconditioning (LIP) against cerebral ischemia/reperfusion injuries.
  
  
  METHODS
  Model of brain ischemia induced by Four-vessel occlusion was used. LIP was performed by clamping the bilateral femoral arteries for 10 min 3 times in a interval of 10 min. Rats with vertebral arteries permanently occluded were divided into sham group, cerebral ischemic group, FNS + cerebral ischemic group, LIP + cerebral ischemic group, FNS + LIP + cerebral ischemic group. The changes of neural density (ND) in the CA1 hippocampus were observed 7d after the sham operation or brain ischemia under thionin staining. The expression of c-Fos in the CA1 hippocampus was measured 6 h after the sham operation or brain ischemia under immunohistochemistry method.
  
  
  RESULTS
  Thionin staining revealed that serious neuronal damage was visualized in the CA1 hippocampus in both cerebral ischemic group and FNS + cerebral ischemic group as compared with sham group. LIP attenuated the neuronal damage of the CA1 subfield induced normally by cerebral ischemia/reperfusion, and ND in LIP + cerebral ischemic group was significantly higher than that in cerebral ischemic group (P < 0.01). But obvious neuronal damage of the CA1 subfield was found in FNS+ LIP + cerebral ischemic group, and ND was significantly decreased as compared with LIP + cerebral ischemic group (P < 0.01). These results suggested that the protection of LIP against cerebral ischemia/reperfusion injuries might be cancelled by preceding section of femoral nerve. It was found that there was almost no c-Fos expression in the CA1 hippocampus in sham group. Changes of c-Fos expression in the CA1 subfield in cerebral ischemic group were similar to that in sham group. But in LIP + cerebral ischemic group, c-Fos expression in the CA1 subfield was markedly increased and the number of positive cells and optical density of c-Fos expression were significantly higher than those in sham and cerebral ischemic group. c-Fos expression in the CA1 subfield was again decreased in FNS + LIP + cerebral ischemic group, and the number of positive cells and optical density of c-Fos expression were significantly lower than those in LIP + cerebral ischemic group.
  
  
  CONCLUSIONS
  Neural pathway participated in the protective effect of LIP on brain, and increased c-Fos expression in the CA1 hippocampus by LIP after cerebral ischemia/reperfusion, might be a part of neural pathway by which LIP induced brain ischemic tolerance.
authors.affiliation: Department of Physiology, Xinxiang Medical College, Xinxiang 453003, China.
authors.forename: Hong-Gang
authors.initials: HG
authors.lastname: Zhao
authors.forename: Wen-Bin
authors.initials: WB
authors.lastname: Li
authors.forename: Xiao-Cai
authors.initials: XC
authors.lastname: Sun
authors.forename: Qing-Jun
authors.initials: QJ
authors.lastname: Li
authors.forename: Ji
authors.initials: J
authors.lastname: Ai
authors.forename: Dong-Liang
authors.initials: DL
authors.lastname: Li
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000503:physiopathology*; D005121:Extremities / Q000098:blood supply; D019194:Ischemic Preconditioning / Q000379:methods; D008297:Male; D009434:Neural Pathways / Q000503:physiopathology*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000503:physiopathology*