title: Effects of erythropoietin on memory deficits and brain oxidative stress in the mouse models of dementia.
abstract: The present study was undertaken to explore the potential of erythropoietin in memory deficits of mice. Memory impairment was produced by scopolamine (0.5 mg/kg, i.p.) and intracerebroventricular streptozotocin (i.c.v STZ, 3 mg/kg, 10 µl, 1(st) and 3(rd) day) in separate groups of animals. Morris water-maze test was employed to assess learning and memory. The levels of brain thio-barbituric acid reactive species (TBARS) and reduced glutathione (GSH) were estimated to assess degree of oxidative stress. Brain acetylcholinesterase enzyme (AChE) activity was also measured. Scopolamine/streptozotocin administration induced significant impairment of learning and memory in mice as indicated by marked decrease in Morris water-maze performance. Scopolamine/streptozotocin administration also produced a significant enhancement of brain AChE activity and brain oxidative stress (an increase in TBARS and a decrease in GSH) levels. Treatment of erythropoietin (500 and 1,000 IU/Kg i.p.) significantly reversed scopolamine- as well as streptozotocin-induced learning and memory deficits along with attenuation of those-induced rise in brain AChE activity and brain oxidative stress levels. It may be concluded that erythropoietin exerts a beneficial effect in memory deficits of mice possibly through its multiple actions including potential anti-oxidative effect.
authors.affiliation: Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala (Punjab), PIN-147002, India.
authors.forename: Rohit
authors.initials: R
authors.lastname: Kumar
authors.forename: Amteshwar Singh
authors.initials: AS
authors.lastname: Jaggi
authors.forename: Nirmal
authors.initials: N
authors.lastname: Singh