title: Complete genome analysis of a novel intertypic recombinant human adenovirus causing epidemic keratoconjunctivitis in Japan.
abstract: For 4 months from September 2008, 102 conjunctival swab specimens were collected for surveillance purposes from patients across Japan suspected of having epidemic keratoconjunctivitis (EKC). Human adenovirus (HAdV) DNA was detected in 61 samples by PCR, though the HAdV type for 6 of the PCR-positive samples could not be determined by phylogenetic analysis using a partial hexon gene sequence. Moreover, for 2 months from January 2009, HAdV strains with identical sequences were isolated from five conjunctival swab samples obtained from EKC patients in five different regions of Japan. For the analyses of the 11 samples mentioned above, we determined the nucleotide sequences of the entire penton base, hexon, and fiber genes and early 3 (E3) region, which are variable regions among HAdV types, and compared them to those of other HAdV species D strains. The nucleotide sequences of loops 1 and 2 in the hexons of all 11 samples showed high degrees of identity with those of the HAdV type 15 (HAdV-15) and HAdV-29 prototype strains. However, the fiber gene and E3 region sequences showed high degrees of identity with those of HAdV-9, and the penton base gene sequence showed a high degree of identity with the penton base gene sequences of HAdV-9 and -26. Moreover, the complete genome sequence of the 2307-S strain, which was isolated by viral culture from 1 of the 11 samples, was determined. The 2307-S strain was a recombinant HAdV between HAdV-9, -15, -26, -29, and/or another HAdV type; however, the recombination sites in the genome were not obvious. We propose that this virus is a novel intertypic recombinant, HAdV-15/29/H9, and may be an etiological agent of EKC.
authors.affiliation: Department of Microbiology, Fukushima Medical University School of Medicine, 1 Hikarigaoka, Fukushima 960-1295, Japan. h-kane@chive.ocn.ne.jp
authors.forename: Hisatoshi
authors.initials: H
authors.lastname: Kaneko
authors.forename: Koki
authors.initials: K
authors.lastname: Aoki
authors.forename: Shigeaki
authors.initials: S
authors.lastname: Ohno
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Ishiko
authors.forename: Tsuguto
authors.initials: T
authors.lastname: Fujimoto
authors.forename: Masayuki
authors.initials: M
authors.lastname: Kikuchi
authors.forename: Seiya
authors.initials: S
authors.lastname: Harada
authors.forename: Gabriel
authors.initials: G
authors.lastname: Gonzalez
authors.forename: Kanako O
authors.initials: KO
authors.lastname: Koyanagi
authors.forename: Hidemi
authors.initials: H
authors.lastname: Watanabe
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Suzutani
mesh_terms: D000257:Adenoviridae Infections / Q000453:epidemiology* / Q000821:virology; D000260:Adenoviruses, Human / Q000235:genetics* / Q000302:isolation & purification*; D016000:Cluster Analysis; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics*; D016679:Genome, Viral; D006801:Humans; D007564:Japan / Q000453:epidemiology; D007637:Keratoconjunctivitis / Q000453:epidemiology* / Q000821:virology; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014764:Viral Proteins / Q000235:genetics
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins