title: [The protective effects of ischemic preconditioning on the kidney injury following with ischemia/reperfusion of limbs and the possible mechanisms].
abstract: OBJECTIVE
  To explore the protective effects of ischemic preconditioning on the kidney injury following with ischemia/reperfusion (I/R) of limbs.
  
  
  METHODS
  The models of I/R injury of limbs were constructed in rabbits. The blood from right external jugular vein, renal artery and renal vein represent the peripheral blood, into and out-flowing kidney blood (IKB, OKB) respectively. Superoxide dismutase (SOD), malondialdehyde (MDA), blood uria nitrogen (BUN) in peripheral blood and SOD, MDA, nitric oxide (NO) in IKB and OKB were measured, as well as SOD, MDA, induced nitric oxide synthase (iNOS) in kidney were detected in different groups. The effects of ischemic preconditioning (IPC) on the kidney injury were observed.
  
  
  RESULTS
  Compared with control group, the activity of SOD in peripheral blood, IKB, OKB and kidney decreased, and the content of MDA increased after 4 h ischemia followed by 4 h reperfusion. The content of BUN in peripheral blood, NO in IKB, OKB and iNOS in kidney increased remarkably as well. SOD increased and MDA, NO, BUN, iNOS decreased significantly by ischemic preconditioning (IPC) before ischemia/reperfusion. The correlation analysis indicated that MDA was negatively correlated with SOD and positively correlated with NO, BUN.
  
  
  CONCLUSIONS
  Oxygen free radicals metabolic confusion of kidney occurred in the course of I/R of limbs, IPC could strengthen the resistance of peroxidation in kidney and had protective effects on the kidney injury following with ischemia/reperfusion (I/ R) of limbs
authors.affiliation: Department of Physiology, North China Coal Medical College, Tangshan 063000, China.
authors.forename: Yan-Lei
authors.initials: YL
authors.lastname: Wang
authors.forename: Chun-Xiu
authors.initials: CX
authors.lastname: Zhao
authors.forename: You-Ling
authors.initials: YL
authors.lastname: Jing
authors.forename: Hui-Ping
authors.initials: HP
authors.lastname: Zheng
authors.forename: Guo-Jin
authors.initials: GJ
authors.lastname: Cui
authors.forename: Shuo-Sen
authors.initials: SS
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001806:Blood Urea Nitrogen; D005121:Extremities / Q000098:blood supply*; D005260:Female; D019194:Ischemic Preconditioning / Q000379:methods*; D007668:Kidney / Q000378:metabolism / Q000503:physiopathology; D015227:Lipid Peroxidation / Q000502:physiology; D008297:Male; D011817:Rabbits; D051437:Renal Insufficiency / Q000209:etiology / Q000517:prevention & control*; D015427:Reperfusion Injury / Q000517:prevention & control*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D013482:Superoxide Dismutase