title: Neuroprotective diarylheptanoids from the leaves and twigs of juglans sinensis against glutamate-induced toxicity in HT22 cells.
abstract: A new diarylheptanoid, juglanin C(1), was isolated from the 80 % methanolic extract of the leaves and twigs of JUGLANS SINENSIS with three known diarylheptanoids, juglanin A(2), juglanin B (3), and (5R)-5-hydroxy-7-(4-hydroxy-3-methoxyphenyl)-1(4-hydroxyphenyl)-3-heptanone (4), using bioactivity-guided fractionation and chromatographic techniques. Among the isolated diarylheptanoids, compounds 1 and 2 significantly showed neuroprotective activities against glutamate-induced toxicity in HT22 cells. These two diarylheptanoids significantly reduced the overproduction of cellular peroxide in glutamate-injured HT22 cells. Moreover, these two diarylheptanoids significantly maintained antioxidative defense systems, including glutathione, glutathione reductase, and glutathione peroxidase, under glutamate-induced oxidative stress in HT22 cells.
authors.affiliation: College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University, Seoul, Korea.
authors.forename: Heejung
authors.initials: H
authors.lastname: Yang
authors.forename: Sang Hyun
authors.initials: SH
authors.lastname: Sung
authors.forename: Jinwoong
authors.initials: J
authors.lastname: Kim
authors.forename: Young Choong
authors.initials: YC
authors.lastname: Kim
mesh_terms: D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology; D036381:Diarylheptanoids / Q000302:isolation & purification / Q000494:pharmacology*; D018698:Glutamic Acid; D005979:Glutathione Peroxidase / Q000096:biosynthesis; D005980:Glutathione Reductase / Q000096:biosynthesis; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism; D031324:Juglans / Q000737:chemistry*; D018696:Neuroprotective Agents / Q000494:pharmacology*; D019906:Nuclear Magnetic Resonance, Biomolecular; D018384:Oxidative Stress / Q000187:drug effects; D010545:Peroxides / Q000378:metabolism; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry
chemical_list: D000975:Antioxidants; D036381:Diarylheptanoids; D018696:Neuroprotective Agents; D010545:Peroxides; D010936:Plant Extracts; D018698:Glutamic Acid; D005979:Glutathione Peroxidase; D005980:Glutathione Reductase