title: A failure of meiotic chromosome segregation in a fbh1Delta mutant correlates with persistent Rad51-DNA associations.
abstract: The F-box DNA helicase Fbh1 constrains homologous recombination in vegetative cells, most likely through an ability to displace the Rad51 recombinase from DNA. Here, we provide the first evidence that Fbh1 also serves a vital meiotic role in fission yeast to promote normal chromosome segregation. In the absence of Fbh1, chromosomes remain entangled or segregate unevenly during meiosis, and genetic and cytological data suggest that this results in part from a failure to efficiently dismantle Rad51 nucleofilaments that form during meiotic double-strand break repair.
authors.affiliation: Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK.
authors.forename: Weili
authors.initials: W
authors.lastname: Sun
authors.forename: Alexander
authors.initials: A
authors.lastname: Lorenz
authors.forename: Fekret
authors.initials: F
authors.lastname: Osman
authors.forename: Matthew C
authors.initials: MC
authors.lastname: Whitby
mesh_terms: D020090:Chromosome Segregation; D053903:DNA Breaks, Double-Stranded; D004265:DNA Helicases / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D004260:DNA Repair; D004271:DNA, Fungal / Q000032:analysis; D004268:DNA-Binding Proteins / Q000235:genetics; D044783:F-Box Proteins / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D005785:Gene Conversion; D017353:Gene Deletion; D008540:Meiosis / Q000235:genetics*; D009687:Nuclear Proteins / Q000032:analysis; D051135:Rad51 Recombinase / Q000032:analysis*; D011995:Recombination, Genetic; D012568:Schizosaccharomyces / Q000235:genetics* / Q000502:physiology; D029702:Schizosaccharomyces pombe Proteins / Q000032:analysis* / Q000235:genetics / Q000502:physiology*; D013172:Spores, Fungal / Q000254:growth & development; D000099257:Meiotic Recombination Protein SPO11
chemical_list: D004265:DNA Helicases; D004271:DNA, Fungal; D004268:DNA-Binding Proteins; D044783:F-Box Proteins; D009687:Nuclear Proteins; D051135:Rad51 Recombinase; D029702:Schizosaccharomyces pombe Proteins; C084232:RHP51 protein, S pombe; C085412:rad52 protein, S pombe; C456270:rec12 protein, S pombe; D000099257:Meiotic Recombination Protein SPO11; C504739:Fbh1 protein, S pombe