title: Leishmania spp. in Didelphis albiventris and Micoureus paraguayanus (Didelphimorphia: Didelphidae) of Brazil.
abstract: Leishmaniasis is kept in nature by the participation of several animal species. This study evaluated the presence of Leishmania spp. in skin samples of free-ranging marsupials Micoureus paraguayanus (n=95) and Didelphis albiventris (n=191), captured in Morro do Diabo State Park and in sections of its surrounding forest, in the region of Pontal do Paranapanema, São Paulo State, Brazil. The samples were tested for the presence of kDNA of Leishmania spp. by polymerase chain reaction (PCR) and by real time PCR (qPCR). All samples from D. albiventris tested by PCR were negative for the presence of kDNA of Leishmania spp. However, when tested by qPCR, the positivity was 1.6%. A positivity of 7.4% by PCR and 11.6% by qPCR was observed for M. paraguayanus. Sixty-four per cent (9/14) of positive animals were limited to the same forest fragment. Presence of Leishmania (Leishmania) amazonensis and Leishmania (Viannia) braziliensis was detected in M. paraguayanus samples. While D. albiventris is the most studied marsupial species due to its urban habits, other marsupial species such as M. paraguayanus can be potential reservoirs of Leishmania spp. and should also be studied.
authors.affiliation: UNESP, University Estadual Paulista, Veterinary Medicine, Rua Clóvis Pestana, 793, Jd. D. Amélia, Araçatuba, São Paulo, CEP 16050-680, Brazil. apnquintal@yahoo.com.br
authors.forename: Amanda Pifano Neto
authors.initials: AP
authors.lastname: Quintal
authors.forename: Erica de Souza
authors.initials: Ede S
authors.lastname: Ribeiro
authors.forename: Fernado Pacheco
authors.initials: FP
authors.lastname: Rodrigues
authors.forename: Flávia Souza
authors.initials: FS
authors.lastname: Rocha
authors.forename: Lucile Maria
authors.initials: LM
authors.lastname: Floeter-Winter
authors.forename: Cáris Maroni
authors.initials: CM
authors.lastname: Nunes
mesh_terms: D000818:Animals; D001938:Brazil / Q000453:epidemiology; D048293:Didelphis; D007891:Leishmania / Q000145:classification*; D007896:Leishmaniasis / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012680:Sensitivity and Specificity