title: Intestinal adaptation for oligopeptide absorption via PepT1 after massive (70%) mid-small bowel resection.
abstract: BACKGROUND
  Proteins are absorbed primarily as short peptides via peptide transporter 1 (PepT1).
  
  
  OBJECTIVE
  Intestinal adaptation for peptide absorption after massive mid-small intestinal resection occurs by increased expression of PepT1 in the remnant small intestine and colon.
  
  
  METHODS
  Peptide uptake was measured in duodenum, jejunum, ileum, and colon using glycyl-sarcosine 1 week (n = 9) and 4 weeks (n = 11) after 70% mid-small bowel resection and in corresponding segments from unoperated rats (n = 12) and after transection and reanastomosis of jejunum and ileum (n = 8). Expression of PepT1 (mRNA, protein) and villus height were measured.
  
  
  RESULTS
  Intestinal transection/reanastomosis did not alter gene expression. Compared to non-operated controls, 70% mid-small bowel resection increased jejunal peptide uptake (p < 0.05) associated with increased villus height (1.13 vs 1.77 and 1.50 mm, respectively, p < 0.01). In ileum although villus height increased at 1 and 4 weeks (1.03 vs 1.21 and 1.35 mm, respectively; p < 0.01), peptide uptake was not altered. PepT1 mRNA and protein were decreased at 1 week, and PepT1 protein continued low at 4 weeks. Gene expression, peptide uptake, and histomorphology were unchanged in the colon.
  
  
  CONCLUSIONS
  Jejunal adaptation for peptide absorption occurs by hyperplasia. Distal ileum and colon do not have a substantive role in adaptation for peptide absorption.
authors.affiliation: Department of Surgery and Gastroenterology Research Unit (GU 10-10), Mayo Clinic, 200 1st St SW, Rochester, MN 55905, USA.
authors.forename: Srivats
authors.initials: S
authors.lastname: Madhavan
authors.forename: Jeffrey S
authors.initials: JS
authors.lastname: Scow
authors.forename: Rizwan M
authors.initials: RM
authors.lastname: Chaudhry
authors.forename: Munenori
authors.initials: M
authors.lastname: Nagao
authors.forename: Ye
authors.initials: Y
authors.lastname: Zheng
authors.forename: Judith A
authors.initials: JA
authors.lastname: Duenes
authors.forename: Michael G
authors.initials: MG
authors.lastname: Sarr
mesh_terms: D000222:Adaptation, Physiological; D000818:Animals; D001835:Body Weight; D003106:Colon / Q000033:anatomy & histology / Q000378:metabolism* / Q000502:physiology; D004386:Duodenum / Q000033:anatomy & histology / Q000378:metabolism* / Q000502:physiology / Q000601:surgery; D007082:Ileum / Q000033:anatomy & histology / Q000378:metabolism* / Q000502:physiology / Q000601:surgery; D007408:Intestinal Absorption / Q000502:physiology*; D007413:Intestinal Mucosa / Q000378:metabolism; D007583:Jejunum / Q000033:anatomy & histology / Q000378:metabolism* / Q000502:physiology / Q000601:surgery; D008297:Male; D000074011:Peptide Transporter 1; D010455:Peptides / Q000493:pharmacokinetics; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D011917:Rats, Inbred Lew; D018709:Statistics, Nonparametric; D027981:Symporters / Q000378:metabolism*
chemical_list: D000074011:Peptide Transporter 1; D010455:Peptides; D012333:RNA, Messenger; C489940:Slc15a1 protein, rat; D027981:Symporters