title: [Effects of hsf1 genotype on the constitutive expression of alphaB-crystallin in mice myocardium].
abstract: OBJECTIVE
  Try to clarify the effects of HSF1 gene on the constitutively expressed alphaBC.
  
  
  METHODS
  To investigate the levels of constitutively expressed alphaB-Crystallin (alphaBC) in hsf1 knockout (hsf1 -/-) and hsf1 wild type (hsf1 +/+) mice myocardium by Western blot and immunohistochemistry.
  
  
  RESULTS
  The alphaBC levels in hsf1 -/- and hsf1 +/+ were 68.42% +/- 4.16%, 100% +/- 7.58%, respectively (P < 0.05, cytosolic fraction), and 20.53% +/- 1.01%, 37.55% +/- 1.91%, respectively (P < 0.05, pellet fraction). The alphaBC signals decreased significantly in hsf1 -/- myocardium compared with hsf1 +/+ myocardium stained with fluorescence immunohistochemistry.
  
  
  CONCLUSIONS
  hsf1 is the important, but not the only factor, which mediates the constitutively expressed alphaBC.
authors.affiliation: Department of Gerontology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.forename: Hong-Hui
authors.initials: HH
authors.lastname: Zhang
authors.forename: Guo-Xian
authors.initials: GX
authors.lastname: Ding
authors.forename: Yun-Lin
authors.initials: YL
authors.lastname: Cheng
authors.forename: Liang-Jun
authors.initials: LJ
authors.lastname: Yan
authors.forename: I J
authors.initials: IJ
authors.lastname: Benjamin
mesh_terms: D000818:Animals; D004268:DNA-Binding Proteins / Q000235:genetics*; D005260:Female; D005838:Genotype; D000076249:Heat Shock Transcription Factors; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D009206:Myocardium / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics*; D038203:alpha-Crystallin B Chain / Q000235:genetics / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; D000076249:Heat Shock Transcription Factors; C490017:Hsf1 protein, mouse; D014157:Transcription Factors; D038203:alpha-Crystallin B Chain