title: Multiple amino acid variations in the nonstructural proteins of swine Japanese encephalitis virus alter its virulence in mice.
abstract: In a previous study, we performed serial brain-to-brain passages of swine Japanese encephalitis virus in mice and sequenced the complete genomes of the F5 and F20 passaged mouse-adapted variants. In the current study, we analyzed the differences between their genome sequences and found 12 amino acid substitutions in the nonstructural proteins. We also assessed the growth characteristics of these two variants in mammalian cells in vitro and in vivo. Our investigations revealed that the F20 variant had enhanced growth characteristics and modified virulence compared with the F5 variant. We therefore conclude that multiple amino acid substitutions in the nonstructural proteins of swine Japanese encephalitis virus alter its virulence in mice.
authors.affiliation: Institute of Animal Husbandry and Veterinary Science, Hubei Academy of Agricultural Sciences, Wuhan, Hubei, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Wu
authors.forename: Yongxiang
authors.initials: Y
authors.lastname: Tian
authors.forename: Junhua
authors.initials: J
authors.lastname: Deng
authors.forename: Keli
authors.initials: K
authors.lastname: Yang
authors.forename: Wangwang
authors.initials: W
authors.lastname: Liang
authors.forename: Rui
authors.initials: R
authors.lastname: Guo
authors.forename: Zhengying
authors.initials: Z
authors.lastname: Duan
authors.forename: Zewen
authors.initials: Z
authors.lastname: Liu
authors.forename: Danna
authors.initials: D
authors.lastname: Zhou
authors.forename: Diping
authors.initials: D
authors.lastname: Xu
mesh_terms: D000220:Adaptation, Biological; D000818:Animals; D001835:Body Weight; D004252:DNA Mutational Analysis; D004664:Encephalitis Virus, Japanese / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity*; D004672:Encephalitis, Japanese / Q000662:veterinary* / Q000821:virology; D051379:Mice; D008807:Mice, Inbred BALB C; D020125:Mutation, Missense; D012367:RNA, Viral / Q000235:genetics; D012692:Serial Passage; D016019:Survival Analysis; D013552:Swine; D013553:Swine Diseases / Q000821:virology*; D017361:Viral Nonstructural Proteins / Q000235:genetics*; D014774:Virulence
chemical_list: D012367:RNA, Viral; D017361:Viral Nonstructural Proteins