title: Dansyl labeling and bidimensional mass spectrometry to investigate protein carbonylation.
abstract: Carbonylation is a non-enzymatic irreversible post-translational modification. The adduction of carbonyl groups to proteins is due to the presence of excess of ROS in cells. Carbonylation of specific amino acid side chains is one of the most abundant consequences of oxidative stress; therefore, the determination of carbonyl groups content in proteins is regarded as a reliable way to estimate the cellular damage caused by oxidative stress. This paper reports a novel RIGhT (Reporter Ion Generating Tag) (A. Amoresano, G. Monti, C. Cirulli, G. Marino. Rapid Commun. Mass Spectrom. 2006, 20, 1400) approach for selective labeling of carbonyl groups in proteins using dansylhydrazide, coupled with selective analysis by bidimensional mass spectrometry. We first applied this approach to ribonuclease A and lysozyme as model proteins. According to the so-called 'gel-free procedures', the analysis is carried out at the level of peptides following tryptic digest of the whole protein mixture. Modified RNaseA was analyzed in combined MS(2) and MS(3) scan mode, to specifically select the dansylated species taking advantage of the dansyl-specific fragmentation pathways. This combination allowed us to obtain a significant increase in signal/noise ratio and a significant increase in sensitivity of analysis, due to the reduction of duty cycle of the mass spectrometer. The unique signal obtained was correlated to peptide 1-10 of RNaseA carbonylated and labeled by dansylhydrazide. This strategy represents the first method leading to the direct identification of the carbonylation sites in proteins, thus indicating the feasibility of this strategy to investigate protein carbonylation in a proteomic approach.
authors.affiliation: Department of Organic Chemistry and Biochemistry, School of Biotechnological Sciences, Federico II University of Naples, Naples, Italy.
authors.forename: Angelo
authors.initials: A
authors.lastname: Palmese
authors.forename: Chiara
authors.initials: C
authors.lastname: De Rosa
authors.forename: Gennaro
authors.initials: G
authors.lastname: Marino
authors.forename: Angela
authors.initials: A
authors.lastname: Amoresano
mesh_terms: D000818:Animals; D002417:Cattle; D010449:Peptide Mapping / Q000379:methods*; D010713:Phosphatidylcholines / Q000737:chemistry*; D050050:Protein Carbonylation; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000737:chemistry; D012259:Ribonuclease, Pancreatic / Q000737:chemistry / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D014357:Trypsin / Q000378:metabolism
chemical_list: C496906:1-myristoyl-2-(12-((5-dimethylamino-1-naphthalenesulfonyl)amino)dodecanoyl)-sn-glycero-3-phosphocholine; D010713:Phosphatidylcholines; D011506:Proteins; D017382:Reactive Oxygen Species; D012259:Ribonuclease, Pancreatic; D014357:Trypsin