title: [Simultaneous HPLC determination of 11 essential compounds in Xuebijing injection].
abstract: OBJECTIVE
  To establish a HPLC method for the determination of 11 essential compounds in Xuebijing injection.
  
  
  METHODS
  Chromatogaphic analysis was performed on Aglient Zorbax SB-C18 (4.6 mm x 250 mm, 5 microm). The mobile phase 0. 5% acetic acid and methanol-acetonitrile-acetic acid (40: 60: 0.5). The flow rate was 1.0 mL x min(-1) with the detection wavelength at 280 nm; column temperature at 35 degrees C, and automatic sampling volume of 20 microL.
  
  
  RESULTS
  All the 11 essential compounds showed good linearity (r = 0.9982-0.9999)in the range of the tests concentration. The RSD of the precision, reproducibility and stability tests were less than 3%; the average recoveries of the method were in the range of 95.02%-104.94%.
  
  
  CONCLUSIONS
  The method is simple, rapid and accurate, so it is proposed for the quality control of compounds of Xuebijing injection.
authors.affiliation: College of Pharmacy, Nankai University, Tianjin 300071, China. jilanxin@126.com
authors.forename: Lanxin
authors.initials: L
authors.lastname: Ji
authors.forename: Hao
authors.initials: H
authors.lastname: Huang
authors.forename: Min
authors.initials: M
authors.lastname: Jiang
authors.forename: Gang
authors.initials: G
authors.lastname: Bai
authors.forename: Guoan
authors.initials: G
authors.lastname: Luo
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004304:Dosage Forms; D004365:Drugs, Chinese Herbal / Q000032:analysis*
chemical_list: D004304:Dosage Forms; D004365:Drugs, Chinese Herbal