title: [Construction of antisense recombinant adenoviral vector for c-myc and its antiproliferative effect on rat lymphocytes].
abstract: OBJECTIVE
  To observe the antiproliferative effect of antisense recombinant adenoviral vector for c-myc on rat thymus lymphocytes.
  
  
  METHODS
  Antisense and sense bacterial plasmids for c-myc were constructed. Bacterial plasmids and El detected adenoviral plasmid were cotransfected into 293 cells. Recombinant adenoviral vectors were obtained after cotransfection. The antiproliferative effects were assayed by MTS. The expression of c-myc mRNA was detected by RT-PCR.
  
  
  RESULTS
  The results showed that antisense recombinant adenoviral vector for c-myc could inhibit rat thymus lymphocytes proliferation. The expression of c-myc mRNA was decreased after antisense recombinant adenoviral vector for c-myc was transfected into cells.
  
  
  CONCLUSIONS
  Recombinant antisense adenoviral vector for c-myc could inhibit rat thymus lymphocytes proliferation.
authors.affiliation: Department of Respiratory Medicine, Xijing Hospital, Fourth Military Medical University, Xi' an 710033, China.
authors.forename: Ying-ge
authors.initials: YG
authors.lastname: Liu
authors.forename: Hao-wen
authors.initials: HW
authors.lastname: Qi
authors.forename: Huan-zhang
authors.initials: HZ
authors.lastname: Li
authors.forename: Ming-quan
authors.initials: MQ
authors.lastname: Su
authors.forename: Wen-bin
authors.initials: WB
authors.lastname: Yu
authors.forename: Yue-yun
authors.initials: YY
authors.lastname: Ma
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D016375:Antisense Elements (Genetics); D002460:Cell Line; D049109:Cell Proliferation; D016259:Genes, myc / Q000235:genetics*; D005822:Genetic Vectors; D008214:Lymphocytes / Q000166:cytology*; D051381:Rats; D013950:Thymus Gland / Q000166:cytology
chemical_list: D016375:Antisense Elements (Genetics)