title: Optimisation of ultra-performance LC conditions using response surface methodology for rapid separation and quantitative determination of phenolic compounds in Artemisia minor.
abstract: A method that couples rapid, sensitive, reproducible and accurate ultra-performance LC (UPLC) with quadrupole-TOF-MS was established for the first simultaneous qualitative and quantitative analysis of phenolic compounds in Artemisia minor. Box-Behnken designs (BBDs) were applied as an effective tool to optimise major parameters that influence the resolution of UPLC, including three gradient steps and column temperature. Under optimal UPLC conditions, a total of 23 phenolic compounds in the crude methanol extracts of A. minor were well separated on a Waters Acquity UPLC BEH C(18) column (100×2.1 mm, 1.7 μm particle size) within 16.5 min, and the compounds were unequivocally or tentatively identified via comparisons with authentic standards and literature. In this study, a total of six major phenolic compounds were quantified in A. minor and the method was validated to be sensitive, precise and accurate within the LOD from 1.24 to 5.27 μg/mL, and the overall intra- and inter-day variations in detection were less than 3.76%. The recovery of the method ranged from 97.9 to 103.8% with RSDs that were less than 5.8%. These results demonstrate that this approach has the potential for quality control of A. minor and other Tibetan herbal medicines.
authors.affiliation: Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhou
authors.forename: Franky
authors.initials: F
authors.lastname: Fung-Kei Choi
authors.forename: Zhi Zhou
authors.initials: ZZ
authors.lastname: He
authors.forename: Jing-Zheng
authors.initials: JZ
authors.lastname: Song
authors.forename: Chun-Feng
authors.initials: CF
authors.lastname: Qiao
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.forename: Li-Sheng
authors.initials: LS
authors.lastname: Ding
authors.forename: Suo-Lang
authors.initials: SL
authors.lastname: Gesang
authors.forename: Hong-Xi
authors.initials: HX
authors.lastname: Xu
mesh_terms: D019306:Artemisia / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods*; D013058:Mass Spectrometry; D010636:Phenols / Q000302:isolation & purification*; D015203:Reproducibility of Results
chemical_list: D010636:Phenols