title: Evaluating age-associated phenotypes in a mouse model of protein dyshomeostasis.
abstract: Proteotoxicity caused by an imbalanced protein quality control surveillance system is believed to contribute to the phenotypes associated with aging as well as many neurodegenerative diseases. Understanding and monitoring the impact of proteotoxicity in these processes offers researchers keen insight into the biology of aging, as well as other conditions that share similar pathological etiologies. In Section 2, we present various technical approaches that can be used to calculate and characterize the phenotypes associated with aging that are linked to increased proteotoxicity. Methods such as the measurement of oligomer protein expression and the capacity of proteasome function are useful tools in observing both aging phenotypes and neurodegenerative diseases, both of which share the phenomenon of impaired protein homeostasis.
authors.affiliation: McAllister Heart Institute, University of North Carolina, Chapel Hill, NC 27599, USA.
authors.forename: Jin-Na
authors.initials: JN
authors.lastname: Min
authors.forename: Cam
authors.initials: C
authors.lastname: Patterson
mesh_terms: D000375:Aging / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D000818:Animals; D001823:Body Composition; D049109:Cell Proliferation; D016922:Cellular Senescence; D004195:Disease Models, Animal; D057075:Enzyme Assays / Q000379:methods; D006706:Homeostasis; D015151:Immunoblotting; D053208:Kaplan-Meier Estimate; D051379:Mice; D019636:Neurodegenerative Diseases / Q000235:genetics / Q000378:metabolism; D018384:Oxidative Stress; D010641:Phenotype; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism; D011506:Proteins / Q000378:metabolism*; D012867:Skin / Q000473:pathology; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D011506:Proteins; D001616:beta-Galactosidase; D046988:Proteasome Endopeptidase Complex; C061553:ATP dependent 26S protease