title: Enzyme entrapped nanoporous scaffolds formed through flow-induced gelation in a microfluidic filter device for sensitive biosensing of organophosphorus compounds.
abstract: A novel and versatile processing method was developed for the formation of gel scaffolds with in situ AChE-AuNPs immobilization for biosensing of organophosphorus compounds. The biosensor designed by our new approach shows high sensitivity, selectivity and reactivation efficiency. This flow-induced immobilization technique opens up new pathways for designing a simple, fast, biocompatible, and cost-effective process for enhanced sensor performance and on-site monitoring of a variety of toxic organophosphorus compounds.
authors.affiliation: Pacific Northwest National Laboratory, Richland, WA 99352, USA.
authors.forename: Donglai
authors.initials: D
authors.lastname: Lu
authors.forename: Guocheng
authors.initials: G
authors.lastname: Shao
authors.forename: Dan
authors.initials: D
authors.lastname: Du
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Limin
authors.initials: L
authors.lastname: Wang
authors.forename: Wanjun
authors.initials: W
authors.lastname: Wang
authors.forename: Yuehe
authors.initials: Y
authors.lastname: Lin
mesh_terms: D001672:Biocompatible Materials; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D004784:Environmental Monitoring; D004800:Enzymes, Immobilized / Q000737:chemistry*; D017022:Flow Injection Analysis / Q000379:methods*; D005782:Gels; D053768:Metal Nanoparticles / Q000737:chemistry; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D009943:Organophosphorus Compounds / Q000032:analysis*
chemical_list: D001672:Biocompatible Materials; D004800:Enzymes, Immobilized; D005782:Gels; D009943:Organophosphorus Compounds