title: Analyses of non-steroidal anti-inflammatory drugs by on-line concentration capillary electrochromatography using poly(stearyl methacrylate-divinylbenzene) monolithic columns.
abstract: This study describes the ability of on-line concentration capillary electrochromatography (CEC) coupled with UV or mass spectrometry (MS) for the determination of nine common non-steroidal anti-inflammatory drugs (NSAIDs) in water samples. A series of poly(stearyl methacrylate-divinylbenzene) (poly(SMA-DVB)) monolithic columns, which were prepared by single step in situ polymerization of divinylbenzene (DVB), stearyl methacrylate (SMA) and vinylbenzenesulfonic acid (VBSA, charged monomer), were developed as separation columns for the first time. The effects of polymerization condition of monolithic columns on analyte separations were examined, and the results indicated that separation performances were markedly improved in monolithic columns prepared with short reaction time (3 h) and low SMA:DVB ratio (40/60 ratio of SMA:DVB). Subsequently, an on-line concentration step of step-gradient elution was combined to this CEC system, and by optimizing the difference in eluent strength between the sample matrix and mobile phase, all NSAIDs detection sensitivity were improved (limit of detection (LOD) was 3.4-10 μg/L for UV, and 0.01-0.19 μg/L for MS). When compared to the best CE and LC reports on NSAIDs analyses so far, this on-line concentration CEC method provided better detection ability within shorter separation time (12 min) when either UV or MS detector was employed. This is the first report for on-line concentration CEC with MS detection applied in trace solute analyses of real samples.
authors.affiliation: Department of Chemistry and Center for Nanotechnology at CYCU, Chung Yuan Christian University, 200 Chung Pei Road, Chung-Li 320, Taiwan.
authors.forename: Chao-Hsiang
authors.initials: CH
authors.lastname: Hsu
authors.forename: Yi-Jie
authors.initials: YJ
authors.lastname: Cheng
authors.forename: Brenda
authors.initials: B
authors.lastname: Singco
authors.forename: Hsi-Ya
authors.initials: HY
authors.lastname: Huang
mesh_terms: D000097:Acetonitriles; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000032:analysis* / Q000302:isolation & purification; D053801:Capillary Electrochromatography / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D008768:Methylmethacrylates / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D058105:Polymerization; D011145:Polyvinyls / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D000097:Acetonitriles; D000894:Anti-Inflammatory Agents, Non-Steroidal; D008768:Methylmethacrylates; D011145:Polyvinyls; C054213:methylmethacrylate-divinylbenzene polymer; C032159:acetonitrile