title: Phylogenetic analysis of the nucleotide sequences for the HN gene of 22 avian paramyxovirus type 2 viruses reveals marked heterogeneity.
abstract: The nucleotide sequence of the HN gene was determined for 21 isolates of avian paramyxovirus type 2 virus and compared with the published HN gene of APMV-2/chicken/California/Yucaipa/56. The HN gene of the 22 viruses had five different lengths in the range of 1737 to 1755 nucleotides coding for 579 to 585 amino acids. Phylogenetic analysis of a corresponding 1734-nucleotide sequence from the HN gene of each virus established five genetic groups (I to V), two of which (II and IV) could be divided into two sub-groups (IIa and IIb; and IVa and IVb). Although there were some exceptions, generally isolates placed in the same genetic group had >80% similarity in nucleotide sequence and <80% with the other isolates; while those in the same sub-group had >90% nucleotide sequence similarity.
authors.affiliation: Virology Department, Veterinary Laboratories Agency-Weybridge, New Haw, Addlestone, UK.
authors.forename: Sahar
authors.initials: S
authors.lastname: Mahmood
authors.forename: Dennis J
authors.initials: DJ
authors.lastname: Alexander
authors.forename: Marek J
authors.initials: MJ
authors.lastname: Slomka
authors.forename: Ruth J
authors.initials: RJ
authors.lastname: Manvell
authors.forename: Amanda
authors.initials: A
authors.lastname: Hanna
authors.forename: Chad M
authors.initials: CM
authors.lastname: Fuller
authors.forename: Ian H
authors.initials: IH
authors.lastname: Brown
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D018109:Avulavirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D045463:Avulavirus Infections / Q000662:veterinary* / Q000821:virology; D001483:Base Sequence; D001715:Bird Diseases / Q000821:virology*; D001717:Birds; D018740:Genetic Heterogeneity; D006389:Hemagglutinins, Viral / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D009439:Neuraminidase / Q000737:chemistry / Q000235:genetics*; D010802:Phylogeny; D016133:Polymerase Chain Reaction
chemical_list: D006389:Hemagglutinins, Viral; D009439:Neuraminidase