title: Incorporation of biaryl units into the 5' and 3' ends of sense and antisense strands of siRNA duplexes improves strand selectivity and nuclease resistance.
abstract: Small interfering RNA (siRNA) is a noncoding RNA with considerable potential as a new therapeutic drug for intractable diseases. siRNAs can be rationally designed and synthesized if the sequences of the disease-causing genes are known. In this paper, we describe the synthesis and properties of siRNAs modified with biaryl units. We found that incorporation of biaryl units into the 5' and 3' ends of sense and antisense strands of siRNA duplexes improved strand selectivity and nuclease resistance.
authors.affiliation: Department of Biomolecular Science, Faculty of Engineering, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
authors.forename: Kayo
authors.initials: K
authors.lastname: Yoshikawa
authors.forename: Aya
authors.initials: A
authors.lastname: Ogata
authors.forename: Chiho
authors.initials: C
authors.lastname: Matsuda
authors.forename: Michinori
authors.initials: M
authors.lastname: Kohara
authors.forename: Hideo
authors.initials: H
authors.lastname: Iba
authors.forename: Yukio
authors.initials: Y
authors.lastname: Kitade
authors.forename: Yoshihito
authors.initials: Y
authors.lastname: Ueno
mesh_terms: D000818:Animals; D001483:Base Sequence; D020868:Gene Silencing; D016174:Hepacivirus / Q000502:physiology; D008156:Luciferases / Q000235:genetics; D016372:RNA, Antisense / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D034741:RNA, Small Interfering / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D012260:Ribonucleases / Q000378:metabolism*; D014779:Virus Replication / Q000235:genetics
chemical_list: D016372:RNA, Antisense; D034741:RNA, Small Interfering; D008156:Luciferases; D012260:Ribonucleases