title: Interaction between DMBT1 and galectin 3 is modulated by the structure of the oligosaccharides carried by DMBT1.
abstract: DMBT1 (deleted in malignant brain tumor 1), a human mucin-like glycoprotein, belonging to the scavenger receptor cysteine-rich (SRCR) superfamily, is mainly secreted from mucosal epithelia. It has been shown previously that interaction of hensin, the rabbit ortholog of DMBT1, with galectin 3, a β-galactoside-binding lectin, induces a terminal differentiation of epithelial cells. In this paper, we have used surface plasmon resonance (SPR), to analyse the binding of galectin 3 to two purified samples of human DMBT1:recombinant DMBT1 produced in CHO cells and DMBT1 isolated from intestinal tissues. Characterization of their glycosylation profile by nano-ESI-Q-TOF tandem mass spectrometry showed significant differences in O-glycans between the two DMBT1 samples. Results obtained by SPR demonstrated that the oligosaccharide side chains of DMBT1 are recognized by the carbohydrate-recognition domain (CRD) of galectin 3 and modification in the pattern of oligosaccharides modulates the binding parameters of DMBT1 with galectin 3. Moreover, using immunohistochemistry on paraffin-embedded colonic tissue sections, we could show a co-localisation of DMBT1 and galectin 3 in human intestine, suggesting a potential physiological interaction.
authors.affiliation: Univ. Lille Nord de France, Lille, France.
authors.forename: Yannick
authors.initials: Y
authors.lastname: Rossez
authors.forename: Bernadette
authors.initials: B
authors.lastname: Coddeville
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Elass
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Quinchon
authors.forename: Olivier
authors.initials: O
authors.lastname: Vidal
authors.forename: Anthony P
authors.initials: AP
authors.lastname: Corfield
authors.forename: Pierre
authors.initials: P
authors.lastname: Gosset
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Lacroix
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Michalski
authors.forename: Catherine
authors.initials: C
authors.lastname: Robbe-Masselot
mesh_terms: D000818:Animals; D016466:CHO Cells; D002135:Calcium-Binding Proteins; D006224:Cricetinae; D003412:Cricetulus; D004268:DNA-Binding Proteins; D037502:Galectin 3 / Q000737:chemistry / Q000378:metabolism*; D006031:Glycosylation; D006801:Humans; D055767:Immobilized Proteins / Q000737:chemistry / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism; D007700:Kinetics; D009844:Oligosaccharides / Q000737:chemistry*; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011956:Receptors, Cell Surface / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D013379:Substrate Specificity; D020349:Surface Plasmon Resonance; D025521:Tumor Suppressor Proteins
chemical_list: D002135:Calcium-Binding Proteins; C108174:DMBT1 protein, human; D004268:DNA-Binding Proteins; D037502:Galectin 3; D055767:Immobilized Proteins; D009844:Oligosaccharides; D011956:Receptors, Cell Surface; D011994:Recombinant Proteins; D025521:Tumor Suppressor Proteins