title: Functional interactions between ubiquitin E2 enzymes and TRIM proteins.
abstract: The TRIM (tripartite motif) family of proteins is characterized by the presence of the tripartite motif module, composed of a RING domain, one or two B-box domains and a coiled-coil region. TRIM proteins are involved in many cellular processes and represent the largest subfamily of RING-containing putative ubiquitin E3 ligases. Whereas their role as E3 ubiquitin ligases has been presumed, and in several cases established, little is known about their specific interactions with the ubiquitin-conjugating E2 enzymes or UBE2s. In the present paper, we report a thorough screening of interactions between the TRIM and UBE2 families. We found a general preference of the TRIM proteins for the D and E classes of UBE2 enzymes, but we also revealed very specific interactions between TRIM9 and UBE2G2, and TRIM32 and UBE2V1/2. Furthermore, we demonstrated that the TRIM E3 activity is only manifest with the UBE2 with which they interact. For most specific interactions, we could also observe subcellular co-localization of the TRIM involved and its cognate UBE2 enzyme, suggesting that the specific selection of TRIM-UBE2 pairs has physiological relevance. Our findings represent the basis for future studies on the specific reactions catalysed by the TRIM E3 ligases to determine the fate of their targets.
authors.affiliation: Cluster in Biomedicine (CBM), AREA Science Park, s.s. 14 km 163.5 Basovizza, 34149 Trieste, Italy.
authors.forename: Luisa M
authors.initials: LM
authors.lastname: Napolitano
authors.forename: Ellis G
authors.initials: EG
authors.lastname: Jaffray
authors.forename: Ronald T
authors.initials: RT
authors.lastname: Hay
authors.forename: Germana
authors.initials: G
authors.lastname: Meroni
mesh_terms: D000595:Amino Acid Sequence; D002478:Cells, Cultured; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D000070584:Tripartite Motif Proteins; D044763:Ubiquitin-Conjugating Enzymes / Q000235:genetics / Q000378:metabolism*; D044767:Ubiquitin-Protein Ligases / Q000235:genetics / Q000378:metabolism*
chemical_list: D009419:Nerve Tissue Proteins; D014157:Transcription Factors; D000070584:Tripartite Motif Proteins; D044763:Ubiquitin-Conjugating Enzymes; C084523:TRIM25 protein, human; C094027:TRIM32 protein, human; C550446:TRIM9 protein, human; D044767:Ubiquitin-Protein Ligases