title: Multilocus sequence typing of Enterococcus faecalis isolates demonstrating different lesion types in broiler breeders.
abstract: A total of 69 isolates of Enterococcus faecalis from broiler breeders demonstrating different lesion types and representing eight different flocks were characterized by multilocus sequence typing. Twenty isolates obtained from healthy birds representing two additional flocks were included for comparison. A total of 12 different sequence types (STs) was obtained. Correlation between ST and lesion type was not demonstrated. However, three STs (ST82, ST174, ST177) made up 81% of the isolates associated with lesions, indicating that these STs might be particularly associated with birds. In addition, ST174, the most frequently demonstrated ST, was only obtained from affected birds. Surprisingly, ST82, previously reported to be associated with amyloid arthropathy in layers worldwide, demonstrated a high degree of diversity as to lesion types, just as healthy carriers were demonstrated among broiler breeders. STs associated with healthy birds and lesions, respectively, did not demonstrate a phylogenetic relationship.
authors.affiliation: Department of Veterinary Disease Biology, Faculty of Life Sciences, University of Copenhagen, 4 Stigbøjlen, Frederiksberg C, Denmark. cava@life.ku.dk
authors.forename: Rikke H
authors.initials: RH
authors.lastname: Gregersen
authors.forename: A
authors.initials: A
authors.lastname: Petersen
authors.forename: Henrik
authors.initials: H
authors.lastname: Christensen
authors.forename: Magne
authors.initials: M
authors.lastname: Bisgaard
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D001483:Base Sequence; D002645:Chickens / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D013293:Enterococcus faecalis / Q000145:classification* / Q000235:genetics* / Q000472:pathogenicity; D005260:Female; D005838:Genotype; D016908:Gram-Positive Bacterial Infections / Q000453:epidemiology / Q000382:microbiology / Q000503:physiopathology / Q000662:veterinary*; D058885:Multilocus Sequence Typing; D016133:Polymerase Chain Reaction; D011201:Poultry Diseases / Q000453:epidemiology / Q000382:microbiology* / Q000503:physiopathology; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial