title: [Doxycycline nano-liposome slow-release gel improves rat periodontitis].
abstract: OBJECTIVE
  To evaluate the therapeutic effects of doxycycline nano-liposome slow-release gel on the periodontitis in an established rat model.
  
  
  METHODS
  The biocompatibility was tested by oral perfusion sample gel for long term observation. Doxycycline nano-gel was used to treat the established rat model of periodontitis. The rats were selected randomly and divided into five groups: blank group, doxycycline nano-gel treated group, minocycline-treated group, matrix group and periodontitis model group. MMP-8 and TIMP-1 in the sulcus fluid were detected by real-time PCR. The data was analysed using SAS13.3 software package for Student's t test.
  
  
  RESULTS
  Doxycycline nano-gel had excellent biocompatibility from weight measure and tissue section evaluation. The results in periodontitis rats demonstrated that doxycycline nano-gel could ameliorate periodontitis condition dramatically compared with the control group, the restoration even better than minocycline-treated group.
  
  
  CONCLUSIONS
  Doxycycline nano-liposome slow-release gel improves rat periodontitis by decreasing MMP-8 level, which provides valuable evidence to promote doxycycline nano-gel clinical application.
authors.affiliation: Department of Stomatology, Zhoupu Hospital, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 201318, China. jinhonglai@sohu.com
authors.forename: Hong-lai
authors.initials: HL
authors.lastname: Jin
authors.forename: Wei
authors.initials: W
authors.lastname: Wu
authors.forename: Rong
authors.initials: R
authors.lastname: Shu
mesh_terms: D000818:Animals; D004318:Doxycycline; D008081:Liposomes; D020784:Matrix Metalloproteinase 8; D010518:Periodontitis; D051381:Rats; D019715:Tissue Inhibitor of Metalloproteinase-1
chemical_list: D008081:Liposomes; C576839:TIMP1 protein, rat; D019715:Tissue Inhibitor of Metalloproteinase-1; D020784:Matrix Metalloproteinase 8; D004318:Doxycycline