title: Determination of the transient period of the EIS complex and investigation of the suppression of blood glucose levels by L-arabinose in healthy adults.
abstract: OBJECTIVE
  L-Arabinose uncompetitively inhibits intestinal sucrase by forming an enzyme-inhibitor-substrate (EIS) complex. The transient period of the EIS complex affects the time span of inhibition. We determined the apparent transient period of the EIS complex of sucrase, L-arabinose, and sucrose both in vitro and in humans.
  
  
  METHODS
  Intestinal acetone powder (a source of sucrase), L-arabinose, and sucrose were mixed and injected into a dialysis membrane that was placed in a sucrose solution. The production rate of D-glucose and the release rate of L-arabinose from sucrase were determined. We also investigated the suppression of blood glucose levels by L-arabinose in 21 healthy volunteers. Sucrose (40 g) was ingested with or without L-arabinose (2 g), then blood glucose values were measured, which returned to steady-state conditions within 2 h. Volunteers were then given 90 g of commercial adzuki bean jelly containing 40 g sucrose as the sucrose load, and blood glucose values were measured again.
  
  
  RESULTS
  Addition of L-arabinose reduced the production rate of D -glucose compared to the rates measured in the absence of L-arabinose for several hours in vitro. L-Arabinose was released at a lower rate in the presence of sucrose than in its absence. Blood glucose values measured 2 h after sucrose was given with L -arabinose were significantly lower than those measured when L-arabinose was not given (Δ change in maximum value: with L-arabinose, 53.8 ± 19.7 mg/dL; without L-arabinose, 65.0 ± 17.7 mg/dL).
  
  
  CONCLUSIONS
  The EIS complex of sucrase-L -arabinose-sucrose was maintained for several hours both in vitro and in humans.
authors.affiliation: Research & Development Laboratory, Sanwa Cornstarch Co. Ltd., 594 Unate, Kashihara-shi, Nara Pref 634-8585, Japan. kiyoshi-shibanuma@sanwa-starch.co.jp
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Shibanuma
authors.forename: Yoko
authors.initials: Y
authors.lastname: Degawa
authors.forename: Koichi
authors.initials: K
authors.lastname: Houda
mesh_terms: D000328:Adult; D001089:Arabinose / Q000008:administration & dosage / Q000378:metabolism*; D001786:Blood Glucose / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D018592:Cross-Over Studies; D003956:Dialysis; D019422:Dietary Sucrose / Q000378:metabolism; D055664:Electrochemical Techniques; D005260:Female; D005947:Glucose / Q000378:metabolism; D006801:Humans; D006943:Hyperglycemia / Q000517:prevention & control*; D007004:Hypoglycemic Agents / Q000008:administration & dosage / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D019518:Postprandial Period; D011485:Protein Binding; D013393:Sucrase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D013395:Sucrose / Q000378:metabolism*; D055815:Young Adult
chemical_list: D001786:Blood Glucose; D019422:Dietary Sucrose; D007004:Hypoglycemic Agents; D013395:Sucrose; D001089:Arabinose; D013393:Sucrase; D005947:Glucose