title: Cell adhesion, morphology and biochemistry on nano-topographic oxidized silicon surfaces.
abstract: Manipulating an incorporated scaffold to direct cell behaviors play a key role in tissue engineering. In this study, we developed novel nano-topographic oxidized silicon nanosponges capable of being modified with various chemicals of a few nm in thickness to gain further insight into the fundamental biology of cell-environment interactions in vitro. A wet etching technique was applied to fabricate the silicon nanosponges in a high-throughput manner and was followed by vapor deposition of various organo-silane chemicals to enable self-assembly on the surfaces of the silicon nanosponges. When Chinese hamster ovary cells were cultured on these chemically modified nano-topographic structures, they displayed distinct morphogenesis, adherent responses, and biochemical properties in comparison with those of their planar oxidized silicon counterparts. There were predominant nano-actin punches and slender protrusions formed while cells were cultured on the nano-topographic structures, indicating that cell behaviors can be influenced by the physical characteristic derived from nano-topography, in addition to the hydrophobicity of contact surfaces. This study demonstrates potential applications of these nano-topographic biomaterials for controlling cell development in tissue engineering as well as in basic cell biology research.
authors.affiliation: Institute of Nanoengineering and MicroSystems, National Tsing Hua University, Hsinchu 300, Taiwan, ROC.
authors.forename: Chung-Yao
authors.initials: CY
authors.lastname: Yang
authors.forename: Lin-Ya
authors.initials: LY
authors.lastname: Huang
authors.forename: Tang-Long
authors.initials: TL
authors.lastname: Shen
authors.forename: J Andrew
authors.initials: JA
authors.lastname: Yeh
mesh_terms: D000818:Animals; D040761:Biomimetic Materials / Q000138:chemical synthesis / Q000737:chemistry*; D016466:CHO Cells; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation; D002470:Cell Survival; D006224:Cricetinae; D003412:Cricetulus; D003599:Cytoskeleton / Q000648:ultrastructure; D051416:Focal Adhesion Protein-Tyrosine Kinases / Q000096:biosynthesis; D057927:Hydrophobic and Hydrophilic Interactions; D049329:Nanostructures; D017646:Organosilicon Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D017646:Organosilicon Compounds; D012822:Silicon Dioxide; D051416:Focal Adhesion Protein-Tyrosine Kinases