title: Activation of the Raf-MEK-ERK pathway is required for neutrophil extracellular trap formation.
abstract: The signaling mechanisms leading to the formation of neutrophil extracellular traps (NETs), relevant in infections, sepsis and autoimmune diseases, are poorly understood. Neutrophils are not amenable to studies with conventional genetic techniques. Using a new chemical genetic analysis we show that the Raf-MEK-ERK pathway is involved in NET formation through activation of NADPH oxidase and upregulation of antiapoptotic proteins. We identify potential targets for drugs addressing NET-associated diseases.
authors.affiliation: Department of Cellular Microbiology, Max Planck Institute for Infection Biology, Berlin.
authors.forename: Abdul
authors.initials: A
authors.lastname: Hakkim
authors.forename: Tobias A
authors.initials: TA
authors.lastname: Fuchs
authors.forename: Nancy E
authors.initials: NE
authors.lastname: Martinez
authors.forename: Simone
authors.initials: S
authors.lastname: Hess
authors.forename: Heino
authors.initials: H
authors.lastname: Prinz
authors.forename: Arturo
authors.initials: A
authors.lastname: Zychlinsky
authors.forename: Herbert
authors.initials: H
authors.lastname: Waldmann
mesh_terms: D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism*; D006801:Humans; D020930:MAP Kinase Kinase Kinases / Q000378:metabolism*; D009504:Neutrophils / Q000378:metabolism*
chemical_list: D048049:Extracellular Signal-Regulated MAP Kinases; D020930:MAP Kinase Kinase Kinases