title: Image cytometry: nuclear and chromosomal DNA quantification.
abstract: Image cytometry (ICM) associates microscopy, digital image and software technologies, and has been particularly useful in spatial and densitometric cytological analyses, such as DNA ploidy and DNA content measurements. Basically, ICM integrates methodologies of optical microscopy calibration, standard density filters, digital CCD camera, and image analysis softwares for quantitative applications. Apart from all system calibration and setup, cytological protocols must provide good slide preparations for efficient and reliable ICM analysis. In this chapter, procedures for ICM applications employed in our laboratory are described. Protocols shown here for human DNA ploidy determination and quantification of nuclear and chromosomal DNA content in plants could be used as described, or adapted for other studies.
authors.affiliation: Laboratory of Cytogenetic and Cytometry, Department of General Biology, Federal University of Viçosa, Viçosa, MG, Brazil. ccarvalh@ufv.br
authors.forename: Carlos Roberto
authors.initials: CR
authors.lastname: Carvalho
authors.forename: Wellington Ronildo
authors.initials: WR
authors.lastname: Clarindo
authors.forename: Isabella Santiago
authors.initials: IS
authors.lastname: Abreu
mesh_terms: D002138:Calibration; D002875:Chromosomes / Q000235:genetics*; D004247:DNA / Q000032:analysis* / Q000648:ultrastructure*; D006801:Humans; D019044:Image Cytometry / Q000379:methods*; D011003:Ploidies; D012394:Rosaniline Dyes
chemical_list: D012394:Rosaniline Dyes; C476677:Schiff's reagent; D004247:DNA