title: [Expression and activity of Cdk5/p35 in a rat model of trigeminal neuropathic pain].
abstract: OBJECTIVE
  To test the hypothesis that Cdk5/p35 plays important roles during trigeminal neuropathic pain.
  
  
  METHODS
  Trigeminal neuralgia rat model was established with a chronic constriction injury (CCI) of the infraorbital branch of the trigeminal nerve (ION). The change of Cdk5 activity, expression of Cdk5 and p35 in Vc after CCI-ION were studied by Western blot, immunoprecipitation and Kinase assay. Statistical analysis was performed using SPSS13.0 software package.
  
  
  RESULTS
  Western blot showed CCI-ION induced a time-dependent upregulation of p35 primarily within the ipsilateral superficial laminae of Vc (day 1:1.23±0.15, day 3:1.36±0.12, day 7:1.62±0.17, day 14:1.83±0.16). In contrast, the expression of Cdk5 was constant during day 1-14 in Vc after CCI-ION. Cdk5 activity on day 14 in Vc after CCI-ION (115.5 Kcpm) was 6 times as that on day 1 in Vc after CCI-ION (19.0 Kcpm). The difference was significant (P<0.01).
  
  
  CONCLUSIONS
  The results suggest that cdk5/p35 may plays important roles on synaptic reorganization of Vc after CCI-ION, expression of p35 may be a novel regulatory mechanism to control cdk5 activity in Vc after CCI-ION.
authors.affiliation: Department of Stomatology, Affiliated Zhujiang Hospital of Southern Medical University, Guangzhou 510282, Guangdong Province, China. liwei19860106@163.com
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Min
authors.initials: M
authors.lastname: Fang
authors.forename: Xiao-hui
authors.initials: XH
authors.lastname: Cai
mesh_terms: D000818:Animals; D051360:Cyclin-Dependent Kinase 5; D009437:Neuralgia; D010770:Phosphotransferases; D051381:Rats; D017207:Rats, Sprague-Dawley; D014277:Trigeminal Neuralgia
chemical_list: C492235:Cdk5r1 protein, rat; D010770:Phosphotransferases; D051360:Cyclin-Dependent Kinase 5; C495907:Cdk5 protein, rat