title: Purification and characterization of organic solvent and detergent tolerant lipase from thermotolerant Bacillus sp. RN2.
abstract: The aim of this study was to characterize the organic solvent and detergent tolerant properties of recombinant lipase isolated from thermotolerant Bacillus sp. RN2 (Lip-SBRN2). The isolation of the lipase-coding gene was achieved by the use of inverse and direct PCR. The complete DNA sequencing of the gene revealed that the lip-SBRN2 gene contains 576 nucleotides which corresponded to 192 deduced amino acids. The purified enzyme was homogeneous with the estimated molecular mass of 19 kDa as determined by SDS-PAGE and gel filtration. The Lip-SBRN2 was stable in a pH range of 9-11 and temperature range of 45-60 °C. The enzyme was a non metallo-monomeric protein and was active against pNP-caprylate (C8) and pNP-laurate (C12) and coconut oil. The Lip-SBRN2 exhibited a high level of activity in the presence of 108% benzene, 102.4% diethylether and 112% SDS. It is anticipated that the organic solvent and detergent tolerant enzyme secreted by Bacillus sp. RN2 will be applicable as catalysts for reaction in the presence of organic solvents and detergents.
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Srinakharinwirot University, Bangkok 10110, Thailand; E-Mails: kanjanavas@hotmail.com (P.K.); wevansi@hotmail.com (S.K.); pkhla@hotmail.com (P.K.); oom_arda@hotmail.com (A.P.).
authors.forename: Pornpimon
authors.initials: P
authors.lastname: Kanjanavas
authors.forename: Sintawee
authors.initials: S
authors.lastname: Khuchareontaworn
authors.forename: Paisarn
authors.initials: P
authors.lastname: Khawsak
authors.forename: Arda
authors.initials: A
authors.lastname: Pakpitcharoen
authors.forename: Khajeenart
authors.initials: K
authors.lastname: Pothivejkul
authors.forename: Somchai
authors.initials: S
authors.lastname: Santiwatanakul
authors.forename: Kenji
authors.initials: K
authors.lastname: Matsui
authors.forename: Tadahiko
authors.initials: T
authors.lastname: Kajiwara
authors.forename: Kosum
authors.initials: K
authors.lastname: Chansiri
mesh_terms: D001407:Bacillus / Q000201:enzymology*; D001426:Bacterial Proteins / Q000737:chemistry*; D003902:Detergents / Q000737:chemistry; D006358:Hot Temperature; D008049:Lipase / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry
chemical_list: D001426:Bacterial Proteins; D003902:Detergents; D012997:Solvents; D008049:Lipase