title: Rab27a is required for human cytomegalovirus assembly.
abstract: Human cytomegalovirus (HCMV) completes its final envelopment on intracellular membranes before it is released from the cell. The mechanisms underlying these processes are not understood. Here we studied the role of Rab27a, a regulator of lysosome-related organelle transport, in HCMV production. HCMV infection increased Rab27a expression, and recruitment of Rab27a to membranous strutures at the assembly site. Immuno-gold labelling demonstrated association of Rab27a with viral envelopes. CMV production was reduced after knock-down of Rab27a, and in Rab27a-deficient ashen melanocytes. This study shows a requirement for Rab27a in the CMV life cycle and suggests that CMV and LRO biogenesis share common molecular mechanisms.
authors.affiliation: Department of Molecular and Cell Biology, Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas, Madrid, Spain. alberto.fraile@cnb.csic.es
authors.forename: Alberto
authors.initials: A
authors.lastname: Fraile-Ramos
authors.forename: Victoria
authors.initials: V
authors.lastname: Cepeda
authors.forename: Edo
authors.initials: E
authors.lastname: Elstak
authors.forename: Peter
authors.initials: P
authors.lastname: van der Sluijs
mesh_terms: D000818:Animals; D001692:Biological Transport; D015153:Blotting, Western; D002460:Cell Line; D003587:Cytomegalovirus / Q000378:metabolism*; D020868:Gene Silencing; D006801:Humans; D007150:Immunohistochemistry; D008247:Lysosomes / Q000378:metabolism; D008544:Melanocytes / Q000166:cytology; D020460:Melanosomes / Q000378:metabolism; D051379:Mice; D008856:Microscopy, Fluorescence / Q000379:methods; D034741:RNA, Small Interfering / Q000378:metabolism; D014759:Viral Envelope Proteins / Q000378:metabolism; D020691:rab GTP-Binding Proteins / Q000502:physiology*; D000075745:rab27 GTP-Binding Proteins
chemical_list: D034741:RNA, Small Interfering; D014759:Viral Envelope Proteins; D000075745:rab27 GTP-Binding Proteins; C066694:RAB27A protein, human; D020691:rab GTP-Binding Proteins