title: [Effect of lactic acid on the survival rate of cultured rat cortex neurons in vitro].
abstract: OBJECTIVE
  To prob the effect of lactic acid on the survival rate of cultured rat cortex neurons.
  
  
  METHODS
  Neurons in primary culture were divided into two parts: (1) Neurons were cultured in physical medium, which was rendered acidotic by addition of lactic acid in rising concentrations. 0 mmol/L, 5 mmol/L, 10 mmol/L, 20 mmol/L, pH range were 7.35, 7.15, 6.95, 6.00. (2) The cells were cultured in physical medium in the presence of lactic acid at increasing concentrations, respectively including lactate 0 mmol/L, 5 mmol/L, 10 mmol/L, 20 mmol/L. They were regulated to maintain pH 7.35. MTT was used to detect the neurons survival rate.
  
  
  RESULTS
  Neurons survival rate significantly decreased with the pH decrease compared with the group pH 7.35. Different lactate concentrations can also reduce the neurons survival rate apparently compared with the group included no lactate. The survival rate of groups pH 7.15 and pH 6.95 were lower than the group in which the lactate concentration was 5 mmol/L and 10 mmol/L.
  
  
  CONCLUSIONS
  It has close relation between pH decrease induced by lactate and neurons survival rate. Lactate can also exert neuron toxicity independently without pH decrease.
authors.affiliation: Institute of Health and Environmental Medicine, Academy of Military Medical Sciences, Tianjin 300050, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Hong-tao
authors.initials: HT
authors.lastname: Liu
authors.forename: Qiang
authors.initials: Q
authors.lastname: Ma
mesh_terms: D000818:Animals; D002470:Cell Survival; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology; D003470:Culture Media / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D019344:Lactic Acid / Q000378:metabolism*; D009474:Neurons / Q000166:cytology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D003470:Culture Media; D019344:Lactic Acid