title: [Research on the interaction between GSTP1 polymorphism and Helicobacter pylori infection in patients with gastric intestinal metaplaisa].
abstract: OBJECTIVE
  To detect the distribution of polymorphism of GSTP1 and infection rate of Helicobacter pylori (H. pylori) in different kinds of gastric intestinal metaplasia (IM) and to explore the relationship between entergenic factor-polymorphism of GSTP1 and extogenic factor-H. pylori infection, to determine the risk of subtype of IM.
  
  
  METHODS
  There were 381 cases in total, including 143 CGS(+) and 238 IM. H. E. stain was used for pathological diagnosis. HID-AB-pH2.5 methods were used to classify the IM. ELISA method was used to detect the antibody of H. pylor-IgG. Polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) were used to analyze the genotype.
  
  
  RESULTS
  Frequency of G genotype was higher in the IM group, when compared to the CGS(+) groups (P < 0.05). The positive rate of H. pylori was statistically higher than CGS(+) (P < 0.01). By effect-modified model analysis, GSTP1 gene polymorphsim and H. pylori infection presented a positive interaction in the stage IM, with the OR value as 9.386 (95%CI: 3.736 - 23.580) after adjusted by age and gender. The synergy index was 2.078 and the attributable proportion of interaction was 46.36%. The positive rate of H. pylori were statistically highter than CGS(+) group in subtype IMI, subtype IMII and III (P < 0.01). The frequency of G genotype was higher in the IMII and III group, when compared with the IMI groups (P < 0.01). By effect-modified model analysis, in the stage of IMII and III, GSTP1 gene polymorphsim and H. pylori infection also presented a positive interaction, with OR value as 24.487 (95%CI: 7.731 - 77.735) after adjusted by age and gender, with its synergy index as 1.844, and attributable proportion of interaction as 43.89%.
  
  
  CONCLUSIONS
  The infection of H. pylori and polymorphism GSTP1gene appeared to be both the external and internal factors, respectively. In the stage of IM, GSTP1 gene polymorphsim and H. pylori infection also presented a positive interaction, expecially in the IMII and III.
authors.affiliation: Cancer Control Laboratory of Cancer Institute and General Surgery, the First Affiliated Hospital of China Medical University, Shenyang 110001, China; Department of Pathology, Shenyang Medical Collage.
authors.forename: Xu-Guang
authors.initials: XG
authors.lastname: Wang
authors.forename: Zhong
authors.initials: Z
authors.lastname: Zhang
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Sun
authors.forename: Ye
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yuan
mesh_terms: D005838:Genotype; D016481:Helicobacter Infections; D016480:Helicobacter pylori / Q000235:genetics; D006801:Humans; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length