title: Denaturing gradient gel electrophoresis analysis with different primers of subgingival bacterial communities under mechanical debridement.
abstract: DGGE of 16S rDNA is one of the most frequently used methods to study microbial communities. In this study, the DGGE profiles of different 16S rDNA regions of the periodontal pathogens Porphyromonas gingivalis, Fusobacterium nucleatum, and Prevotella nigrescens were investigated. The results suggested that V3-V5 and V6-V8 fragments may be suitable for community analysis of subgingival bacteria. Further analysis of subgingival samples with V3-V5 and V6-V8 regions as target fragments suggested that, in chronic periodontitis, re-colonization by periodontal bacteria with a population very similar to the baseline may occur by 6 weeks after mechanical debridement.
authors.affiliation: Department of Periodontology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai 200011, China.
authors.forename: Dali
authors.initials: D
authors.lastname: Liu
authors.forename: Yanbin
authors.initials: Y
authors.lastname: Zhou
authors.forename: Chaolun
authors.initials: C
authors.lastname: Li
authors.forename: Yunpeng
authors.initials: Y
authors.lastname: Li
authors.forename: Yuntao
authors.initials: Y
authors.lastname: Jiang
authors.forename: Zhengwei
authors.initials: Z
authors.lastname: Huang
authors.forename: Jingping
authors.initials: J
authors.lastname: Liang
authors.forename: Rong
authors.initials: R
authors.lastname: Shu
mesh_terms: D000328:Adult; D001419:Bacteria / Q000302:isolation & purification*; D002908:Chronic Disease; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D058645:Denaturing Gradient Gel Electrophoresis / Q000379:methods*; D005260:Female; D005881:Gingiva / Q000382:microbiology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D057747:Periodontal Debridement; D010518:Periodontitis / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S