title: Antibodies anti-Saccharomyces cerevisiae (ASCA) do not differentiate Crohn's disease from celiac disease.
abstract: BACKGROUND
  Anti-Saccharomyces cerevisiae antibodies (ASCA), considered serologic markers for Crohn's disease, were described in patients with celiac disease, disappearing after a gluten-free diet.
  
  
  OBJECTIVE
  Evaluation of ASCA positivity in patients with Crohn's disease and celiac disease in relation to healthy individuals.
  
  
  METHODS
  A total of 145 individuals were studied: 36 with Crohn's disease and 52 with celiac disease, that fulfilled the diagnostic criteria for both affections, and 57 healthy individuals for control. The celiac patients were divided as follow: group CeD I at diagnosis (n = 34), group CeD II with gluten-free diet compliance (n = 13) and group CeD III with transgressions to the diet (n = 5). ASCA IgA and IgG were determined by ELISA.
  
  
  RESULTS
  With statistical significance, ASCA IgA were positive in Crohn's disease, celiac disease at diagnosis and celiac disease with diet transgressions; ASCA IgG in Crohn's disease and in all groups with celiac disease.
  
  
  CONCLUSIONS
  The detection of ASCA in patients with celiac disease allows to suggest that ASCA is not a specific marker for Crohn's disease, but was associated with the inflammation of the small intestine. The increased levels of positive ASCA may be due to genetic factors and increased intestinal permeability.
authors.affiliation: Hospital Cajuru, Pontifícia Universidade Católica do Paraná, Brazil. loretekotze@hotmail.com
authors.forename: Lorete Maria da Silva
authors.initials: LM
authors.lastname: Kotze
authors.forename: Renato Mitsunori
authors.initials: RM
authors.lastname: Nisihara
authors.forename: Shirley Ramos da Rosa
authors.initials: SR
authors.lastname: Utiyama
authors.forename: Paulo Gustavo
authors.initials: PG
authors.lastname: Kotze
authors.forename: Petra Mirella
authors.initials: PM
authors.lastname: Theiss
authors.forename: Márcia
authors.initials: M
authors.lastname: Olandoski
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000908:Antibodies, Fungal / Q000097:blood*; D001323:Autoantibodies / Q000097:blood; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D002446:Celiac Disease / Q000175:diagnosis* / Q000276:immunology; D003424:Crohn Disease / Q000175:diagnosis* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D008297:Male; D008875:Middle Aged; D012441:Saccharomyces cerevisiae / Q000276:immunology*; D055815:Young Adult
chemical_list: D000908:Antibodies, Fungal; D001323:Autoantibodies; D015415:Biomarkers; D007074:Immunoglobulin G; D007075:Immunoglobulin M