title: Assembling a puzzle of dispersed retrotransposable sequences in the genome of chickpea (Cicer arietinum L.).
abstract: Several repetitive elements are known to be present in the genome of chickpea (Cicer arietinum L.) including satellite DNA and En/Spm transposons as well as two dispersed, highly repetitive elements, CaRep1 and CaRep2. PCR was used to prove that CaRep1, CaRep2, and previously isolated CaRep3 of C. arietinum represent different segments of a highly repetitive Ty3-gypsy-like retrotransposon (Metaviridae) designated CaRep that makes up large parts of the intercalary heterochromatin. The full sequence of this element including the LTRs and untranslated internal regions was isolated by selective amplification. The restriction pattern of CaRep was different within the annual species of the genus Cicer, suggesting its rearrangement during the evolution of the genus during the last 100 000 years. In addition to CaRep, another LTR and a non-LTR retrotransposon family were isolated, and their restriction patterns and physical localization in the chickpea genome were characterized. The LINE-like element CaLin is only of comparatively low abundance and reveals a considerable heterogeneity. The Ty1-copia-like element (Pseudoviridae) CaTy is located in the distal parts of the intercalary heterochromatin and adjacent euchromatic regions, but it is absent from the centromeric regions. These results together with earlier findings allow to depict the distribution of retroelements on chickpea chromosomes, which extensively resembles the retroelement landscape of the genome of the model legume Medicago truncatula Gaertn.
authors.affiliation: Molecular BioSciences, Biocentre, University of Frankfurt am Main, Max-von-Laue-Straße 9, D-60438 Frankfurt am Main, Germany. staginnus@em.uni-frankfurt.de
authors.forename: C
authors.initials: C
authors.lastname: Staginnus
authors.forename: C
authors.initials: C
authors.lastname: Desel
authors.forename: T
authors.initials: T
authors.lastname: Schmidt
authors.forename: G
authors.initials: G
authors.lastname: Kahl
mesh_terms: D000595:Amino Acid Sequence; D029863:Cicer / Q000145:classification / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics; D018745:Genome, Plant / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D015183:Restriction Mapping; D018626:Retroelements / Q000235:genetics*; D016415:Sequence Alignment; D020079:Terminal Repeat Sequences
chemical_list: D018744:DNA, Plant; D018626:Retroelements