title: Histone and TK0471/TrmBL2 form a novel heterogeneous genome architecture in the hyperthermophilic archaeon Thermococcus kodakarensis.
abstract: Being distinct from bacteria and eukaryotes, Archaea constitute a third domain of living things. The DNA replication, transcription, and translation machineries of Archaea are more similar to those of eukaryotes, whereas the genes involved in metabolic processes show more similarity to their bacterial counterparts. We report here that TK0471/TrmB-like 2 (TrmBL2), in addition to histone, is a novel type of abundant chromosomal protein in the model euryarchaeon Thermococcus kodakarensis . The chromosome of T. kodakarensis can be separated into regions enriched either with histone, in which the genetic material takes on a “beads-on-a-string” appearance, or with TK0471/TrmBL2, in which it assumes a thick fibrous structure. TK0471/TrmBL2 binds to both coding and intergenic regions and represses transcription when bound to the promoter region. These results show that the archaeal chromosome is organized into heterogeneous structures and that TK0471/TrmBL2 acts as a general chromosomal protein as well as a global transcriptional repressor.
authors.affiliation: Laboratory of Plasma Membrane and Nuclear Signaling, Graduate School of Biostudies, Kyoto University, Kyoto, Japan. maruyama@lif.kyoto-u.ac.jp
authors.forename: Hugo
authors.initials: H
authors.lastname: Maruyama
authors.forename: Minsang
authors.initials: M
authors.lastname: Shin
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Oda
authors.forename: Rie
authors.initials: R
authors.lastname: Matsumi
authors.forename: Ryosuke L
authors.initials: RL
authors.lastname: Ohniwa
authors.forename: Takehiko
authors.initials: T
authors.lastname: Itoh
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Shirahige
authors.forename: Tadayuki
authors.initials: T
authors.lastname: Imanaka
authors.forename: Haruyuki
authors.initials: H
authors.lastname: Atomi
authors.forename: Shige H
authors.initials: SH
authors.lastname: Yoshimura
authors.forename: Kunio
authors.initials: K
authors.lastname: Takeyasu
mesh_terms: D019843:Archaeal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D002868:Chromosomal Proteins, Non-Histone / Q000737:chemistry / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D019847:Chromosomes, Archaeal / Q000648:ultrastructure*; D019641:DNA, Archaeal / Q000737:chemistry / Q000648:ultrastructure; D017353:Gene Deletion; D019848:Gene Expression Regulation, Archaeal; D020745:Genome, Archaeal; D006657:Histones / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D019712:Thermococcus / Q000235:genetics* / Q000648:ultrastructure; D015854:Up-Regulation
chemical_list: D019843:Archaeal Proteins; D002868:Chromosomal Proteins, Non-Histone; D019641:DNA, Archaeal; D006657:Histones