title: Recent advances in the MS analysis of glycoproteins: Capillary and microfluidic workflows.
abstract: Recent developments in bioanalytical instrumentation, MS detection, and computational data analysis approaches have provided researchers with capabilities for interrogating the complex cellular glycoproteome, to help gain a better insight into the cellular and physiological processes that are associated with a disease and to facilitate the efforts centered on identifying disease-specific biomarkers. This review describes the progress achieved in the characterization of protein glycosylation by using advanced capillary and microfluidic MS technologies. The major steps involved in large-scale glycoproteomic analysis approaches are discussed, with special emphasis given to workflows that have evolved around complex MS detection functions. In addition, quantitative analysis strategies are assessed, and the bioinformatics aspects of glycoproteomic data processing are summarized. The developments in commercial and custom fabricated microfluidic front-end platforms to ESI- and MALDI-MS instrumentation, for addressing major challenges in carbohydrate analysis such as sensitivity, throughput, and ability to perform structural characterization, are further evaluated and illustrated with relevant examples.
authors.affiliation: Virginia Bioinformatics Institute and Virginia Polytechnic Institute and State University, Blacksburg, VA, USA.
authors.forename: Diego F
authors.initials: DF
authors.lastname: Cortes
authors.forename: Jarod L
authors.initials: JL
authors.lastname: Kabulski
authors.forename: Alexandru C
authors.initials: AC
authors.lastname: Lazar
authors.forename: Iulia M
authors.initials: IM
authors.lastname: Lazar
mesh_terms: D019075:Electrophoresis, Capillary / Q000379:methods* / Q000639:trends; D006023:Glycoproteins / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D013058:Mass Spectrometry / Q000379:methods* / Q000639:trends; D046210:Microfluidic Analytical Techniques / Q000379:methods* / Q000639:trends; D040901:Proteomics / Q000379:methods* / Q000639:trends
chemical_list: D006023:Glycoproteins