title: Rapid hydrophilic interaction chromatography determination of lysine in pharmaceutical preparations with fluorescence detection after postcolumn derivatization with o-phtaldialdehyde.
abstract: A rapid procedure for the determination of lysine based on hydrophilic interaction chromatography (HILIC) separation of arginine and lysine with fluorescence detection has been developed. The separation conditions and parameters of lysine postcolumn derivatization with o-phtaldialdehyde (OPA)/2-mercaptoethanol were studied. The various HILIC columns were employed using isocratic elution. Fluorescence detection was performed at excitation and emission wavelength of 345 nm and 450 nm, respectively. An advantage of the reported method is a simple sample pre-treatment and a quick and very sensitive HPLC method. The developed method was successfully applied for analysis of commercial samples of Ibalgin Fast tablets (Zentiva, Czech Republic).
authors.affiliation: Zentiva, as Praha, U Kabelovny 130, 102 37 Praha 10, Czech Republic. hplc@hplc.cz
authors.forename: Michal
authors.initials: M
authors.lastname: Douša
authors.forename: Jiří
authors.initials: J
authors.lastname: Břicháč
authors.forename: Petr
authors.initials: P
authors.lastname: Gibala
authors.forename: Petr
authors.initials: P
authors.lastname: Lehnert
mesh_terms: D001120:Arginine / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D057927:Hydrophobic and Hydrophilic Interactions; D007202:Indicators and Reagents; D057230:Limit of Detection; D008239:Lysine / Q000302:isolation & purification*; D008623:Mercaptoethanol / Q000737:chemistry; D004364:Pharmaceutical Preparations / Q000032:analysis*; D012015:Reference Standards; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence; D013997:Time Factors; D009764:o-Phthalaldehyde / Q000737:chemistry*
chemical_list: D007202:Indicators and Reagents; D004364:Pharmaceutical Preparations; D008623:Mercaptoethanol; D009764:o-Phthalaldehyde; D001120:Arginine; D008239:Lysine