title: [Effect of ligustrazine on expression of Fas/FasL in pulmonary injury induced by ischemia/reperfusion in rabbits].
abstract: OBJECTIVE
  To investigate the effect of ligustrazine (LGT) on expression of Fas/FasL mRNA during pulmonary ischemia/reperfusion injury (PI/RI) in the rabbits.
  
  
  METHODS
  Single lung ischemia/reperfusion animal model was used in this study. The rabbits were randomly divided into three groups (n = 30, in each): sham operated group (Sham), I/R group (I/R) and I/R + LGT group (I/R + LGT). Changes of several parameters which included apoptotic index (AI), wet to dry ratio of lung tissue weight (W/D) and index of quantitative assessment of histologic lung injury (IQA) were measured at 1h, 3h, 5h after reperfusion in lung tissue. Meanwhile the location and expression of Fas/FasL mRNA were observed. Lung tissue was prepared for light microscopic and electron microscopic ob servation at 1 h, 3 h, 5 h after reperfusion.
  
  
  RESULTS
  As compared with group I/R, Fas/FasL mRNA slightly expressed in intima and extima of small pulmonary artery, alveoli, and bronchiole epithelia in group LGT. The values of AI, W/D and IQA showed significantly lower in group I/R + LGT than that in group I/R at 1 h, 3 h, 5 h after reperfusion in lung tissue (P < 0.01 and P < 0.05). Meanwhile, abnormal changes of the lung tissue in morphologically were lessen markedly in group I/R + LGT.
  
  
  CONCLUSIONS
  Ligustrazine has notable protective effects on PI/RI in rabbits by inhibiting Fas/FasL mRNA express in lung tissue and decreasing apoptosis.
authors.affiliation: Department of Pathophysiology, Wenzhou Medical College, Wenzhou 325027, China. wzwwt@tom.com
authors.forename: Wan-Tie
authors.initials: WT
authors.lastname: Wang
authors.forename: Xiao-Yang
authors.initials: XY
authors.lastname: Wang
authors.forename: Rui-Jie
authors.initials: RJ
authors.lastname: Chen
authors.forename: Xi-Wen
authors.initials: XW
authors.lastname: Chen
authors.forename: Mao-Lin
authors.initials: ML
authors.lastname: Hao
authors.forename: Fang-Yan
authors.initials: FY
authors.lastname: Wang
authors.forename: Zhou-Xi
authors.initials: ZX
authors.lastname: Fang
mesh_terms: D000818:Animals; D017209:Apoptosis; D053222:Fas Ligand Protein / Q000378:metabolism*; D008168:Lung / Q000098:blood supply; D055370:Lung Injury / Q000378:metabolism* / Q000473:pathology; D011719:Pyrazines / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics; D011817:Rabbits; D015427:Reperfusion Injury / Q000378:metabolism* / Q000473:pathology; D019014:fas Receptor / Q000378:metabolism*
chemical_list: D053222:Fas Ligand Protein; D011719:Pyrazines; D012333:RNA, Messenger; D019014:fas Receptor; C017953:tetramethylpyrazine