title: Efficient production of butyric acid from Jerusalem artichoke by immobilized Clostridium tyrobutyricum in a fibrous-bed bioreactor.
abstract: Butyric acid is an important specialty chemical with wide industrial applications. The feasible large-scale fermentation for the economical production of butyric acid requires low-cost substrate and efficient process. In the present study, butyric acid production by immobilized Clostridium tyrobutyricum was successfully performed in a fibrous-bed bioreactor using Jerusalem artichoke as the substrate. Repeated-batch fermentation was carried out to produce butyric acid with a high butyrate yield (0.44 g/g), high productivity (2.75 g/L/h) and a butyrate concentration of 27.5 g/L. Furthermore, fed-batch fermentation using sulfuric acid pretreated Jerusalem artichoke hydrolysate resulted in a high butyric acid concentration of 60.4 g/L, with the yield of 0.38 g/g and the selectivity of ∼ 85.1 (85.1g butyric acid/g acetic acid). Thus, the production of butyric acid from Jerusalem artichoke on a commercial scale could be achieved based on the system developed in this work.
authors.affiliation: Institute of Bioengineering, Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Huang
authors.forename: Jin
authors.initials: J
authors.lastname: Cai
authors.forename: Jin
authors.initials: J
authors.lastname: Wang
authors.forename: Xiangcheng
authors.initials: X
authors.lastname: Zhu
authors.forename: Lei
authors.initials: L
authors.lastname: Huang
authors.forename: Shang-Tian
authors.initials: ST
authors.lastname: Yang
authors.forename: Zhinan
authors.initials: Z
authors.lastname: Xu
mesh_terms: D019342:Acetic Acid / Q000737:chemistry; D019149:Bioreactors; D001709:Biotechnology / Q000379:methods*; D020148:Butyric Acid / Q000737:chemistry; D018914:Cells, Immobilized / Q000378:metabolism*; D048014:Clostridium tyrobutyricum / Q000378:metabolism*; D004867:Equipment Design; D005285:Fermentation; D005632:Fructose / Q000737:chemistry; D005947:Glucose / Q000737:chemistry; D006368:Helianthus / Q000378:metabolism*; D010578:Petroleum; D013997:Time Factors
chemical_list: D010578:Petroleum; D020148:Butyric Acid; D005632:Fructose; D005947:Glucose; D019342:Acetic Acid