title: Patterns of recombination activity on mouse chromosome 11 revealed by high resolution mapping.
abstract: The success of high resolution genetic mapping of disease predisposition and quantitative trait loci in humans and experimental animals depends on the positions of key crossover events around the gene of interest. In mammals, the majority of recombination occurs at highly delimited 1-2 kb long sites known as recombination hotspots, whose locations and activities are distributed unevenly along the chromosomes and are tightly regulated in a sex specific manner. The factors determining the location of hotspots started to emerge with the finding of PRDM9 as a major hotspot regulator in mammals, however, additional factors modulating hotspot activity and sex specificity are yet to be defined. To address this limitation, we have collected and mapped the locations of 4829 crossover events occurring on mouse chromosome 11 in 5858 meioses of male and female reciprocal F1 hybrids of C57BL/6J and CAST/EiJ mice. This chromosome was chosen for its medium size and high gene density and provided a comparison with our previous analysis of recombination on the longest mouse chromosome 1. Crossovers were mapped to an average resolution of 127 kb, and thirteen hotspots were mapped to <8 kb. Most crossovers occurred in a small number of the most active hotspots. Females had higher recombination rate than males as a consequence of differences in crossover interference and regional variation of sex specific rates along the chromosome. Comparison with chromosome 1 showed that recombination events tend to be positioned in similar fashion along the centromere-telomere axis but independently of the local gene density. It appears that mammalian recombination is regulated on at least three levels, chromosome-wide, regional, and at individual hotspots, and these regulation levels are influenced by sex and genetic background but not by gene content.
authors.affiliation: Center for Genome Dynamics, The Jackson Laboratory, Bar Harbor, Maine, United States of America.
authors.forename: Timothy
authors.initials: T
authors.lastname: Billings
authors.forename: Evelyn E
authors.initials: EE
authors.lastname: Sargent
authors.forename: Jin P
authors.initials: JP
authors.lastname: Szatkiewicz
authors.forename: Nicole
authors.initials: N
authors.lastname: Leahy
authors.forename: Il-Youp
authors.initials: IY
authors.lastname: Kwak
authors.forename: Nazira
authors.initials: N
authors.lastname: Bektassova
authors.forename: Michael
authors.initials: M
authors.lastname: Walker
authors.forename: Terry
authors.initials: T
authors.lastname: Hassold
authors.forename: Joel H
authors.initials: JH
authors.lastname: Graber
authors.forename: Karl W
authors.initials: KW
authors.lastname: Broman
authors.forename: Petko M
authors.initials: PM
authors.lastname: Petkov
mesh_terms: D000483:Alleles; D000818:Animals; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D003434:Crossing Over, Genetic; D005260:Female; D016678:Genome; D005838:Genotype; D008297:Male; D008540:Meiosis; D051379:Mice; D008810:Mice, Inbred C57BL; D008957:Models, Genetic; D011995:Recombination, Genetic