title: Comparison of real-time polymerase chain reaction and DNA-strip technology in microbiological evaluation of periodontitis treatment.
abstract: The impact of a semiquantitative commercially available test based on DNA-strip technology (microIDent®, Hain Lifescience, Nehren, Germany) on diagnosis and treatment of severe chronic periodontitis of 25 periodontitis patients was evaluated in comparison with a quantitative in-house real-time PCR. Subgingival plaque samples were collected at baseline as well as at 3, 6, and 12 months later. After extracting DNA, Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola, and several other periodontopathogens were determined by both methods. The results obtained by DNA-strip technology were analyzed semiquantitatively and additionally quantitatively by densitometry. The results for the 4 major periodontopathogenic bacterial species correlated significantly between the 2 methods. Samples detecting a high bacterial load by one method and negative by the other were always found in less than 2% of the total samples. Both technologies showed the impact of treatment on microflora. Especially the semiquantitative DNA-strip technology clearly analyzed the different loads of periodontopathogens after therapy and is useful in microbial diagnostics for patients in dental practices.
authors.affiliation: Institute of Medical Microbiology, University Hospital of Jena, Jena, Germany. sigrun.eick@zmk.unibe.ch
authors.forename: Sigrun
authors.initials: S
authors.lastname: Eick
authors.forename: Anna
authors.initials: A
authors.lastname: Straube
authors.forename: Arndt
authors.initials: A
authors.lastname: Guentsch
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Pfister
authors.forename: Holger
authors.initials: H
authors.lastname: Jentsch
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001419:Bacteria / Q000235:genetics* / Q000302:isolation & purification*; D058491:Bacterial Load; D001431:Bacteriological Techniques / Q000379:methods*; D016903:Drug Monitoring / Q000379:methods; D005260:Female; D005858:Germany; D006801:Humans; D008297:Male; D008875:Middle Aged; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D010518:Periodontitis / Q000382:microbiology* / Q000628:therapy*