title: [Effect of ginkgolides on gene expression of HIF-1alpha in primary cultured neurons].
abstract: OBJECTIVE
  To study the effects of ginkgolides (Gin) on the expression of hypoxia inducible factor-1 (HIF-1alpha) in hypoxic/ischemic neurons.
  
  
  METHODS
  The gene expression of HIF-1alpha pretreated with or without Gin (37.5 microg/ml) was observed by RT-PCR on primary cultured cortical neurons in the condition of hypoxia and oxygen-glucose deprivation.
  
  
  RESULTS
  Some basic expression of HIF-1alpha mRNA were observed in cultured cortical neurons. The expression of HIF-1alpha mRNA increased after 24 h treatment with Gin. The level of HIF-la mRNA increased also after 1 h hypoxia and further enhanced after the pretreatment with Gin. The expression of HIF-1alpha mRNA decreased with the deprivation of both oxygen and glucose, which reversed after the pretreatment of Gin.
  
  
  CONCLUSIONS
  Gin could increase the expression of HIF-1alpha mRNA in hypoxic/ischemic cortical neurons.
authors.affiliation: Nantong University, School of Medicine, Institute of Nautical Medicine, Nantong, 226001, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhu
authors.forename: Xiao-Mei
authors.initials: XM
authors.lastname: Wu
authors.forename: Jian-Cheng
authors.initials: JC
authors.lastname: Li
authors.forename: Shu-Yi
authors.initials: SY
authors.lastname: Jin
mesh_terms: D000818:Animals; D015687:Cell Hypoxia; D015870:Gene Expression; D046934:Ginkgolides / Q000494:pharmacology*; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008813:Mice, Inbred ICR; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D046934:Ginkgolides; C497443:Hif1a protein, mouse; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D012333:RNA, Messenger