title: [Expression of AMPA receptors and related protein in immobilization stressed rats and effect of Xiaoyaosan].
abstract: OBJECTIVE
  To observe protein expression changes of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor and related regulatory protein in the hippocampus and amygdala in chronic immobilization stressed rat and Xiaoyaosan's regulatory effect.
  
  
  METHODS
  Rats were tied 3 h per day to establish immobilization stress condition and treatment with Xiaoyaosan. After 7 days and 21 days stress, the protein expression of AMPA receptor subunit (GluR2/3), N-ethylmaleimide sensitive factor (NSF) and protein interacting with C-kinase 1 (PICK1) in hippocampus and amygdala were detected by using Western blot techniques.
  
  
  RESULTS
  The expression of GluR2/3, NSF in dentate gyrus (DG) and amygdala were markedly attenuated (P < 0.05) and PICK1 in CA1 region were significantly increased (P < 0.05) in 7 d immobilization stressed rats while 7 days xiaoyaosan treatment showed an effective regulatory result to PICK1's changes. Under 21 days immobilization stressed condition, the expression of GluR2/3, NSF in CA1 region showed an increasing trend, and GluR2/3 showed a markedly increase (P < 0.01), but showed an significantly decreased trend in amygdala, Xiaoyaosan showed an effective result to such changes above (P < 0.05). The expression of PICK1 showed increasing trend in amygdala and xiaoyaosan could lower its expression (P < 0.05).
  
  
  CONCLUSIONS
  There are different trends of the expression of AMPA receptor in repeat short-term stress versus chronic immobilization stress, and in hippocampal CA1 region versus amygdala. Xiaoyaosan has better regulation effect on the expression of AMPA receptors in the condition of chronic immobilization stress than those of repeat shortterm stress.
authors.affiliation: Department of Chinese Diagnosis, Beijing University of Chinese Medcine, Beijing 100029, China.
authors.forename: Guang-Xin
authors.initials: GX
authors.lastname: Yue
authors.forename: Zhu-Feng
authors.initials: ZF
authors.lastname: Wang
authors.forename: Qiao-Li
authors.initials: QL
authors.lastname: Zhang
authors.forename: Xin
authors.initials: X
authors.lastname: Zhao
authors.forename: Li-Feng
authors.initials: LF
authors.lastname: Yue
authors.forename: Jie
authors.initials: J
authors.lastname: Ding
authors.forename: Jia-Xu
authors.initials: JX
authors.lastname: Chen
mesh_terms: D000679:Amygdala / Q000378:metabolism*; D000818:Animals; D056547:CA1 Region, Hippocampal / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism; D003598:Cytoskeletal Proteins; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D008297:Male; D052066:N-Ethylmaleimide-Sensitive Proteins / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D018091:Receptors, AMPA / Q000378:metabolism*; D012149:Restraint, Physical; D013315:Stress, Psychological
chemical_list: D002352:Carrier Proteins; D003598:Cytoskeletal Proteins; D004365:Drugs, Chinese Herbal; D009687:Nuclear Proteins; C498298:PICK1 protein, rat; D018091:Receptors, AMPA; C532268:xiaoyaosan; D052066:N-Ethylmaleimide-Sensitive Proteins