title: Expression of Numb and Numb-like in the development of mammalian auditory sensory epithelium.
abstract: The Numb and Numb-like are evolutionarily conserved cell fate-determining factors in mammals. For the first time, we investigate the involvement of the Numb and Numb-like in the developing auditory sensory epithelium. We show that both of them are expressed in the rat auditory sensory epithelium, and the four isoforms of the Numb have dynamic expression patterns during cochlear development. At the early stage of the auditory epithelium development, they occur in all progenitor cells. At the late stage of cell differentiation, they are expressed mainly in the cytoplasm of apical cells and their locations are different. Furthermore, we find overexpression of the Numb or Numb-like, in cochlear whole mount cultures, can upregulate mRNA level of Rath1, which is important in the hair-cell development.
authors.affiliation: Department of Otology and Skull Base Surgery, Eye and ENT Hospital, Fudan University, Shanghai, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Gao
authors.forename: Fang-Lu
authors.initials: FL
authors.lastname: Chi
authors.forename: Yi-Bo
authors.initials: YB
authors.lastname: Huang
authors.forename: Juan-Mei
authors.initials: JM
authors.lastname: Yang
authors.forename: Ning
authors.initials: N
authors.lastname: Cong
authors.forename: Wen
authors.initials: W
authors.lastname: Li
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001306:Auditory Pathways / Q000254:growth & development* / Q000502:physiology; D015153:Blotting, Western; D003051:Cochlea / Q000033:anatomy & histology / Q000254:growth & development; D018076:DNA, Complementary / Q000096:biosynthesis / Q000235:genetics; D004848:Epithelium / Q000254:growth & development* / Q000502:physiology; D005455:Fluorescent Antibody Technique; D006198:Hair Cells, Auditory / Q000378:metabolism; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics*; D018613:Microscopy, Confocal; D009924:Organ Culture Techniques; D010957:Plasmids / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D018076:DNA, Complementary; D047908:Intracellular Signaling Peptides and Proteins; C506057:Numbl protein, rat; D012333:RNA, Messenger; C559994:numb protein, rat