title: Phosphoprotein affinity purification identifies proteins involved in S-adenosyl-L-methionine-induced enhancement of antibiotic production in Streptomyces coelicolor.
abstract: Streptomycetes are the major natural source of clinical antibiotics. The enhanced secondary metabolite production of many streptomycetes by S-adenosylmethionine (SAM) in previous studies suggested the existence of a common SAM regulatory effect. We screened nine proteins using the phosphoprotein purification column from Streptomyces coelicolor. Among them, genes (SCO5477, SCO5113, SCO4647, SCO4885 and SCO1793) for five proteins were disrupted by insertion mutation. The undecylprodigiosin and actinorhodin productions were changed in all mutations. The SAM-induced enhancement of actinorhodin production was abolished by all mutations except SCO4885 mutation, which reduced the production of actinorhodin and undecylprodigiosin with SAM treatment. This study demonstrates that phosphoprotein affinity purification can be used as a screening method to identify the proteins involved SAM signaling.
authors.affiliation: Division of Bioscience and Bioinformatics, College of Natural Science, Myongji University, Gyeonggi-Do, Korea.
authors.forename: Lingzhu
authors.initials: L
authors.lastname: Meng
authors.forename: Seung Hwan
authors.initials: SH
authors.lastname: Yang
authors.forename: Sasikumar Arunachalam
authors.initials: SA
authors.lastname: Palaniyandi
authors.forename: Sung-Kwon
authors.initials: SK
authors.lastname: Lee
authors.forename: In-Ae
authors.initials: IA
authors.lastname: Lee
authors.forename: Tae-Jong
authors.initials: TJ
authors.lastname: Kim
authors.forename: Joo-Won
authors.initials: JW
authors.lastname: Suh
mesh_terms: D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification; D002846:Chromatography, Affinity; D004269:DNA, Bacterial / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D016254:Mutagenesis, Insertional; D010750:Phosphoproteins / Q000737:chemistry* / Q000378:metabolism; D012436:S-Adenosylmethionine / Q000494:pharmacology*; D048372:Streptomyces coelicolor / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D053719:Tandem Mass Spectrometry
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D010750:Phosphoproteins; D012436:S-Adenosylmethionine