title: Dynamic expression patterns of imprinted genes in human embryonic stem cells following prolonged passaging and differentiation.
abstract: OBJECTIVE
  To evaluate the overall expression patterns of imprinted genes in human embryonic stem cells following long term culture and differentiation.
  
  
  METHODS
  Expression levels of 65 imprinted genes determined by PCR array were analyzed in one human embryonic stem cell line (cHES1) following prolonged passaging and differentiation.
  
  
  RESULTS
  Transcripts of 63 imprinted genes were detected in cHES1 cells. Expression levels of all but 5 imprinted genes did not correlate with passage numbers or differ in cells after passage 50 compared with those before passage 50. SLC22A2, SLC22A3, CPA, H19, COPG2IT1 and IGF2 expression were significantly increased in embryoid bodies compared with undifferentiated cells.
  
  
  CONCLUSIONS
  The global expression profiles of imprinted genes are generally stable in human embryonic stem cells after prolonged passaging and differentiation.
authors.affiliation: Reproductive Medical Center, The First Affiliated Hospital of Sun Yat-Sen University, 58 Zhongshan 2nd Avenue, Yuexiu, 510080, Guangzhou, China.
authors.forename: Xiuyun
authors.initials: X
authors.lastname: Mai
authors.forename: Qingyun
authors.initials: Q
authors.lastname: Mai
authors.forename: Tao
authors.initials: T
authors.lastname: Li
authors.forename: Canquan
authors.initials: C
authors.lastname: Zhou
mesh_terms: D002454:Cell Differentiation / Q000235:genetics*; D002460:Cell Line; D053595:Embryonic Stem Cells / Q000166:cytology*; D057890:Epigenomics / Q000379:methods; D005786:Gene Expression Regulation; D015894:Genome, Human; D018392:Genomic Imprinting; D006801:Humans; D007335:Insulin-Like Growth Factor II / Q000235:genetics / Q000378:metabolism; D027701:Organic Cation Transport Proteins / Q000235:genetics / Q000378:metabolism; D000075102:Organic Cation Transporter 2; D062085:RNA, Long Noncoding; D022661:RNA, Untranslated / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic; D000099254:Organic Cation Transporter 3
chemical_list: D007335:Insulin-Like Growth Factor II; D027701:Organic Cation Transport Proteins; D000075102:Organic Cation Transporter 2; D062085:RNA, Long Noncoding; D022661:RNA, Untranslated; D000099254:Organic Cation Transporter 3; C094335:H19 long non-coding RNA; C035170:IGF2 protein, human; C491013:SLC22A2 protein, human