title: 2-DE proteomic analysis of HSP70 in mollusc Chamelea gallina.
abstract: Bidimensional electrophoresis (2-DE) protocols were adapted on Chamelea gallina digestive glands studies by the analysis of Heat Shock Proteins (HSP) compared with monodimensional electrophoresis (1-DE) results. Because polycyclic aromatic hydrocarbons (PAH) act on HSPs, C. gallina specimens were exposed to 0.5 mg/L of benzo[a]pyrene (B[a]P) for 24 h, 7 and 12 days. Immunoblotting after 1-DE showed a single band of 70 kDa significantly induced after 7 days of B[a]P exposure. After 2-DE, eight major high-resolved spots between 17 and 98 kDa were revealed. Three spots fell within the range of 62-98 kDa and of 5-6 pI, parameters which could include HSP70. Two spots of 77 and 72 kDa, obtained after 2-DE immunoblotting, could correspond to constitutive HSC70 and to inducible HSP70 forms respectively. Changes observed in inducible and in constitutive forms might be related to an adaptation to stress and to a normal protein synthesis capability, respectively. Employment of 2-DE and relationship between HSP70 and HSC70 may be useful to clarify their role in molluscs subjected to stress events.
authors.affiliation: Department of Biochemistry "G. Moruzzi", University of Bologna, Via Tolara di Sopra 50, 40064 Ozzano Emilia, Bologna, Italy.
authors.forename: Foschi
authors.initials: F
authors.lastname: Jurgen
authors.forename: Matozzo
authors.initials: M
authors.lastname: Valerio
authors.forename: Rosmini
authors.initials: R
authors.lastname: Roberto
authors.forename: Serrazanetti Gian
authors.initials: SG
authors.lastname: Paolo
authors.forename: Monari
authors.initials: M
authors.lastname: Marta
mesh_terms: D000818:Animals; D001564:Benzo(a)pyrene / Q000494:pharmacology; D004064:Digestive System / Q000187:drug effects / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D005786:Gene Expression Regulation / Q000187:drug effects; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D008974:Mollusca / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics; D014874:Water Pollutants, Chemical / Q000494:pharmacology
chemical_list: D018840:HSP70 Heat-Shock Proteins; D014874:Water Pollutants, Chemical; D001564:Benzo(a)pyrene