title: [Effect of Safflor yellow against neonatal hypoxia on expression of NOSs mRNA].
abstract: OBJECTIVE
  To investigate the effect of Safflor yellow on the gene expression of neuronal nitric oxide synthase (nNOS), inducible nitric oxide synthase (iNOS) and endothelial nitric oxide synthase (eNOS) in neonatal asphyxia.
  
  
  METHODS
  30 minutes after SY 7 g/kg weight intraperitoneally was administered on the neonatal rats. After asphyxia for 40 minutes,the neonatal rats were reoxygenated for 48 h, and the nitric oxide synthases (NOSs) mRNA expression was assessed by semi-quantitative reverse transcription-polymerase chain reaction.
  
  
  RESULTS
  Neuronal nitric oxide synthase (nNOS) and inducible nitric oxide synthase (iNOS) were up in hypoxia/reoxygenation (H/R) 48 h group, while both of them were down significantly in SY group, but no change was observed on endothelial nitric oxide synthase (eNOS).
  
  
  CONCLUSIONS
  The protective of SY from brain damage induced by neonatal asphyxia might be associated with expression of NOSs mRNA.
authors.affiliation: Department of Biochemistry, Nantong Medical College, Nantong 226001.
authors.forename: Dong-Mei
authors.initials: DM
authors.lastname: Yin
authors.forename: Shu-Yi
authors.initials: SY
authors.lastname: Jin
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D002599:Chalcone / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use; D015870:Gene Expression; D000860:Hypoxia / Q000378:metabolism* / Q000517:prevention & control; D052248:Nitric Oxide Synthase Type I / Q000378:metabolism*; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism*; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism*; D011809:Quinones / Q000494:pharmacology* / Q000627:therapeutic use; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D011809:Quinones; D012333:RNA, Messenger; C085278:hydroxysafflor yellow A; D002599:Chalcone; D052248:Nitric Oxide Synthase Type I; D052247:Nitric Oxide Synthase Type II; D052250:Nitric Oxide Synthase Type III; C496322:Nos1 protein, rat; C496318:Nos2 protein, rat; C496316:Nos3 protein, rat