title: [Interleukin-8 inhibits clonal expansion of 3T3-L1 preadipocyte during differentiation].
abstract: OBJECTIVE
  To investigate the effect of interleukin-8 (IL-8) on the differentiation and clonal expansion of 3T3-L1 preadipocyte during the differentiation period.
  
  
  METHODS
  The morphological changes of 3T3-L1 cells during differentiation after the treatment of IL-8 was observed by Oil-Red O staining. Glycerol-3-phosphate dehydrogenase (GPDH) activity was measured by a spectrophotometric method. MTT method and 3H-TdR incorporation were applied to examine the changes of cell proliferation and DNA synthesis in clonal expansion of 3T3-L1 cells. Cell cycle analysis was taken by flow cytometry.
  
  
  RESULTS
  IL-8 could inhibit the differentiation and GDPH activity in a dose dependent manner. IL-8 decreased the cell proliferation and DNA synthesis in clonal expansion after induction. Also, the proportion of cells in G1 phase was increased and that of cells in S and G2 phase was declined after the treatment of IL-8.
  
  
  CONCLUSIONS
  IL-8 inhibits the differentiation of 3T3-L1 preadipocytes by decreasing the clonal expansion of the cells.
authors.affiliation: Life Science Division, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055.
authors.forename: Hua
authors.initials: H
authors.lastname: Zhou
authors.forename: Xi
authors.initials: X
authors.lastname: Yang
authors.forename: Ya-Ou
authors.initials: YO
authors.lastname: Zhang
authors.forename: Guo-Ping
authors.initials: GP
authors.lastname: Cai
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology* / Q000378:metabolism; D000818:Animals; D002453:Cell Cycle; D002454:Cell Differentiation; D049109:Cell Proliferation / Q000187:drug effects*; D016209:Interleukin-8 / Q000494:pharmacology*; D051379:Mice
chemical_list: D016209:Interleukin-8