title: Relationship between neuropathology and disease progression in the SOD1(G93A) ALS mouse.
abstract: Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease characterized by the progressive loss of upper and lower motor neurons. However, recent reports suggest an active role of non-neuronal cells in the pathogenesis of the disease. Here, we examined quantitatively the temporal development of neuropathologic features in the brain and spinal cord of a mouse model of ALS (SOD1(G93A)). Four phases of the disease were studied in both male and female SOD1(G93A) mice: presymptomatic (PRE-SYM), symptomatic (SYM), endstage (ES) and moribund (MB). Compared to their control littermates, SOD1(G93A) mice showed an increase in astrogliosis in the motor cortex, spinal cord and motor trigeminal nucleus in the SYM phase that worsened progressively in ES and MB animals. Associated with this increase in astrogliosis was a concomitant increase in motor neuron cell death in the spinal cord and motor trigeminal nucleus in both ES and MB mice, as well as in the ventrolateral thalamus in MB animals. In contrast, microglial activation was significantly increased in all the same regions but only when the mice were in the MB phase. These results suggest that astrogliosis preceded or occurred concurrently with neuronal degeneration whereas prominent microgliosis was evident later (MB stage), after significant motor neuron degeneration had occurred. Hence, our findings support a role for astrocytes in modulating the progression of non-cell autonomous degeneration of motor neurons, with microglia playing a role in clearing degenerating neurons.
authors.affiliation: Genzyme Corporation, 49 New York Ave, Framingham, MA 01701-9322, USA. wendy.yang@genzyme.com
authors.forename: Wendy W
authors.initials: WW
authors.lastname: Yang
authors.forename: Richard L
authors.initials: RL
authors.lastname: Sidman
authors.forename: Tatyana V
authors.initials: TV
authors.lastname: Taksir
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Treleaven
authors.forename: Jonathan A
authors.initials: JA
authors.lastname: Fidler
authors.forename: Seng H
authors.initials: SH
authors.lastname: Cheng
authors.forename: James C
authors.initials: JC
authors.lastname: Dodge
authors.forename: Lamya S
authors.initials: LS
authors.lastname: Shihabuddin
mesh_terms: D000409:Alanine / Q000235:genetics; D019943:Amino Acid Substitution / Q000235:genetics; D000690:Amyotrophic Lateral Sclerosis / Q000201:enzymology* / Q000235:genetics / Q000473:pathology*; D000818:Animals; D004195:Disease Models, Animal; D018450:Disease Progression; D005260:Female; D005998:Glycine / Q000235:genetics; D006801:Humans; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D013482:Superoxide Dismutase / Q000096:biosynthesis* / Q000235:genetics
chemical_list: C109415:SOD1 G93A protein; D013482:Superoxide Dismutase; D000409:Alanine; D005998:Glycine