title: Florfenicol inhibits allergic airway inflammation in mice by p38 MAPK-mediated phosphorylation of GATA 3.
abstract: Florfenicol has been shown to possess anti-inflammatory activity. However, its possible use for asthma has not yet been studied. First we investigated the anti-inflammatory properties of florfenicol using mice asthma model. BALB/c mice were immunized and challenged by ovalbumin. Treatment with florfenicol caused a marked reduction in inflammatory cells and three Th2 type cytokines in the bronchoalveolar lavage fluids of mice. The levels of ovalbumin-specific IgE and airway hyperresponsiveness were significantly altered after treatment with florfenicol. Histological studies using H&E and AB-PAS staining demonstrate that florfenicol substantially inhibited ovalbumin-induced inflammatory cells infiltration in lung tissue and goblet cell hyperplasia in the airway. These results were similar to those obtained with dexamethasone treatment. We then investigated which signal transduction mechanisms could be implicated in florfenicol activity. Our results suggested that the protective effect of florfenicol was mediated by the inhibition of the p38 MAPK-mediated phosphorylation of GATA 3.
authors.affiliation: Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun, 130062, People's Republic of China.
authors.forename: Ci
authors.initials: C
authors.lastname: Xinxin
authors.forename: Chen
authors.initials: C
authors.lastname: Chi
authors.forename: Chu
authors.initials: C
authors.lastname: Xiao
authors.forename: Xu
authors.initials: X
authors.lastname: Xue
authors.forename: Yang
authors.initials: Y
authors.lastname: Yongjun
authors.forename: Cui
authors.initials: C
authors.lastname: Junqing
authors.forename: Deng
authors.initials: D
authors.lastname: Xuming
mesh_terms: D021581:Active Transport, Cell Nucleus / Q000187:drug effects; D000818:Animals; D000900:Anti-Bacterial Agents / Q000627:therapeutic use*; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000627:therapeutic use*; D001249:Asthma / Q000188:drug therapy* / Q000201:enzymology; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D016207:Cytokines / Q000032:analysis; D003907:Dexamethasone / Q000494:pharmacology; D004195:Disease Models, Animal; D004804:Eosinophils / Q000187:drug effects; D005260:Female; D050990:GATA3 Transcription Factor / Q000378:metabolism*; D020397:Goblet Cells / Q000187:drug effects; D007073:Immunoglobulin E / Q000032:analysis; D008168:Lung / Q000187:drug effects; D008264:Macrophages / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D009504:Neutrophils / Q000187:drug effects; D010766:Phosphorylation; D018418:Th2 Cells / Q000187:drug effects; D013839:Thiamphenicol / Q000031:analogs & derivatives* / Q000627:therapeutic use; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D000894:Anti-Inflammatory Agents, Non-Steroidal; D016207:Cytokines; D050990:GATA3 Transcription Factor; D007073:Immunoglobulin E; D003907:Dexamethasone; C035534:florfenicol; D048051:p38 Mitogen-Activated Protein Kinases; D013839:Thiamphenicol