title: IPL irradiation rejuvenates skin collagen via the bidirectional regulation of MMP-1 and TGF-β1 mediated by MAPKs in fibroblasts.
abstract: The efficacy of intense pulsed light (IPL) in remodeling the extracellular matrix of aged skin had been proven by an increasing number of clinical trials. However, because of the lack of research about the underlying molecular and signaling mechanisms, its efficiency had not been accepted universally. A potential mechanism of IPL rejuvenation effects is due to its different effects on diverse cytokines, the impact of IPL on them may determine the phenotype and prognosis of the aged skin. We designed this study to evaluate the impact of IPL on the secretion of matrix metalloproteinase-1 (MMP-1), transforming growth factor-β1 (TGF-β1), and the mitogen-activated protein kinase (MAPK) signaling pathway in human skin fibroblasts, and tried to study the respective functions of MAPKs as mediators of the MMP-1, TGF-β1 secretion. Results showed that the MMP-1 secretion was only enhanced by IPL at 10 J/cm(2); while the TGF-β1 secretion was inhibited by IPL when the fluence was below 36 J/cm(2), but enhanced at 72 J/cm(2). Meanwhile, ERK inhibitor PD98059 decreased MMP-1 secretion, but did not show a significant influence on TGF-β1; JNK inhibitor SP600125 increased the secretion of MMP-1 and decreased the TGF-β1 secretion; P38 inhibitor SB203580 had no significant influence on MMP-1 but increased the secretion of TGF-β1. Our findings indicated that the bidirectional influence of IPL on the secretion of MMP-1 and TGF-β1 is a potential mechanism of its skin rejuvenation effect; and the secretion of these two cytokines can be mediated by MAPKs.
authors.affiliation: Department of Dermatology, The Third Xiangya Hospital of Central South University, Changsha, 410013, Hunan Province, People's Republic of China. dermalhjh@yahoo.com
authors.forename: Jinhua
authors.initials: J
authors.lastname: Huang
authors.forename: Xiang
authors.initials: X
authors.lastname: Luo
authors.forename: Jianyun
authors.initials: J
authors.lastname: Lu
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.forename: Chengxin
authors.initials: C
authors.lastname: Zuo
authors.forename: Yaping
authors.initials: Y
authors.lastname: Xiang
authors.forename: Shengbo
authors.initials: S
authors.lastname: Yang
authors.forename: Lina
authors.initials: L
authors.lastname: Tan
authors.forename: Jian
authors.initials: J
authors.lastname: Kang
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Bi
mesh_terms: D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism / Q000528:radiation effects; D006801:Humans; D053685:Laser Therapy; D020935:MAP Kinase Signaling System / Q000528:radiation effects; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism; D008954:Models, Biological; D015398:Signal Transduction / Q000528:radiation effects; D012867:Skin / Q000378:metabolism / Q000528:radiation effects; D015595:Skin Aging / Q000502:physiology* / Q000528:radiation effects*; D053773:Transforming Growth Factor beta1 / Q000378:metabolism
chemical_list: D053773:Transforming Growth Factor beta1; D003094:Collagen; C522360:MMP1 protein, human; D020781:Matrix Metalloproteinase 1