title: Dynamin 2 associates with microtubules at mitosis and regulates cell cycle progression.
abstract: Dynamin, a ~100 kDa large GTPase, is known as a key player for membrane traffic. Recent evidence shows that dynamin also regulates the dynamic instability of microtubules by a mechanism independent of membrane traffic. As microtubules are highly dynamic during mitosis, we investigated whether the regulation of microtubules by dynamin is essential for cell cycle progression. Dynamin 2 intensely localized at the mitotic spindle, and the localization depended on its proline-rich domain (PRD), which is required for microtubule association. The deletion of PRD resulted in the impairment of cytokinesis, whereby the mutant had less effect on endocytosis. Interestingly, dominant-negative dynamin (K44A), which blocks membrane traffic but has no effect on microtubules, also blocked cytokinesis. On the other hand, the deletion of the middle domain, which binds to γ-tubulin, impaired the entry into mitosis. As both deletion mutants had no significant effect on endocytosis, dynamin 2 may participate in cell cycle progression by regulating the microtubules. These data suggest that dynamin may play a key role for cell cycle progression by two distinct pathways, membrane traffic and cytoskeleton.
authors.affiliation: Department of Neuroscience, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences.
authors.forename: Nobuhisa
authors.initials: N
authors.lastname: Ishida
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Nakamura
authors.forename: Kenji
authors.initials: K
authors.lastname: Tanabe
authors.forename: Shun-Ai
authors.initials: SA
authors.lastname: Li
authors.forename: Kohji
authors.initials: K
authors.lastname: Takei
mesh_terms: D002455:Cell Division; D048749:Cytokinesis; D034285:Dynamin II / Q000032:analysis* / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004705:Endocytosis; D006367:HeLa Cells; D006801:Humans; D008870:Microtubules / Q000378:metabolism*; D008938:Mitosis; D009154:Mutation; D017434:Protein Structure, Tertiary; D008941:Spindle Apparatus / Q000378:metabolism
chemical_list: D034285:Dynamin II