title: [Effects of Chinese herbal medicine Yiqi Huayu Recipe on apoptosis of dorsal root ganglion neurons and expression of caspase-3 in rats with lumbar nerve root compression].
abstract: OBJECTIVE
  To observe the effects of Yiqi Huayu Recipe, a Chinese compound herbal medicine, on apoptosis of dorsal root ganglion (DRG) neurons and expression of caspase-3 in rats after lumbar nerve root compression injury.
  
  
  METHODS
  A total of 40 male Sprague-Dawley rats were randomly allocated into 4 groups: control group, untreated group, Methylcobal group and Yiqi Huayu Recipe group. Surgery was performed on rats of untreated group, Methylcobal group and Yiqi Huayu Recipe group to place a micro-silica gel on right L₄ DRG, while control group received skin and paravertebral muscle incision only. Rats in Methylcobal group and Yiqi Huayu Recipe group were given Methylcobal by intramuscular injection and Yiqi Huayu Recipe intragastrically respectively. Rats in control group and untreated group received saline intragastrically as equal amount as Yiqi Huayu Recipe group. The compressed nerve roots were harvested at the 10th day after treatment. Apoptosis of DRG neurons was detected by terminal deoxynucleotidyl transferase-mediated nick-end labeling. Caspase-3 activity and mRNA expression in compressed nerve roots were detected with spectrophotography and real-time polymerase chain reaction respectively.
  
  
  RESULTS
  Apoptosis of DRG neurons was significantly increased in the rat model. The apoptosis index of untreated group was higher than that of control group (P<0.01). Yiqi Huayu Recipe and Methylcobal could reduce the apoptosis of DRG neurons, and both groups showed a lower apoptosis index than untreated group (P<0.01). Caspase-3 activity and its gene expression were significantly increased in untreated group. The levels of caspase-3 activity and its gene expression in untreated group were higher than those in control group (P<0.05 or P<0.01). Yiqi Huayu Recipe and Methylcobal could reduce the overexpression of caspase-3 mRNA, and statistically significant differences were found between the untreated group and Yiqi Huayu Recipe group or Methylcobal group (P<0.01).
  
  
  CONCLUSIONS
  Lumbar nerve root compression results in overexpression of caspase-3 in nerve root tissue and increase of DRG neuron apoptosis. Yiqi Huayu Recipe can inhibit the overexpression of caspase-3 and alleviate the apoptosis of DRG neurons after nerve injury.
authors.affiliation: Institute of Spine, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, China.
authors.forename: Le-qin
authors.initials: LQ
authors.lastname: Xu
authors.forename: Xiao-feng
authors.initials: XF
authors.lastname: Li
authors.forename: You-wei
authors.initials: YW
authors.lastname: Zhang
authors.forename: Bing
authors.initials: B
authors.lastname: Shu
authors.forename: Qi
authors.initials: Q
authors.lastname: Shi
authors.forename: Yong-jun
authors.initials: YJ
authors.lastname: Wang
authors.forename: Chong-jian
authors.initials: CJ
authors.lastname: Zhou
mesh_terms: D000818:Animals; D017209:Apoptosis; D053148:Caspase 3 / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005727:Ganglia, Spinal / Q000166:cytology; D008297:Male; D009474:Neurons / Q000187:drug effects*; D011843:Radiculopathy / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013117:Spinal Cord Compression / Q000378:metabolism / Q000473:pathology
chemical_list: D004365:Drugs, Chinese Herbal; C505410:Casp3 protein, rat; D053148:Caspase 3