title: Survival of transplanted rat bone marrow-derived osteogenic stem cells in vivo.
abstract: This study was designed to trace bone marrow-derived stromal cells (MSC) after implantation in an ectopic rat model of bone tissue engineering. MSC were isolated from adult donor rats, expanded, seeded on a hydroxyapatite/β-tricalcium phosphate bone graft substitute (Straumann® BoneCeramic), and cultivated until confluent. Before subcutaneous implantation of seeded constructs and controls (unseeded bone graft substitute) in isogenic rats (n = 32), cells were labeled with the fluorescent dye carboxyfluoresceine-diacetate-succinimidyl-ester. Specimens were harvested at sacrifice on day 1, 3, 7, or 14 after implantation (n = 8 per group) and processed for histology (hematoxylin and eosin, CD68, 4',6-diamidino-2-phenylindol). Carboxyfluoresceine-diacetate-succinimidyl-ester-labeled transplanted cells were quantified in decalcified sections (50 fields of view per specimen) at 488 nm. Over time, transplanted cells decreased in number from 31.3 ± 2.3 (day 1) to 9.2 ± 1.1 (day 3) and 0.3 ± 0.1 (day 7) (p < 0.001). Fourteen days postimplantation MSC could no longer be identified. Additionally, starting on day 3 postimplantation, cellular disintegration was noted. Multinucleated giant cells were present in constructs and controls on day 7 and increased to day 14 postimplantation. These results indicate that ectopically transplanted MSC survive for a rather short time after implantation. Possible reasons for early cell death are discussed.
authors.affiliation: Department of Oral and Craniomaxillofacial Surgery, University Hospital Schleswig-Holstein Campus Kiel, Kiel, Germany. c.e.zimmermann@gmx.de
authors.forename: Corinna E
authors.initials: CE
authors.lastname: Zimmermann
authors.forename: Matthias
authors.initials: M
authors.lastname: Gierloff
authors.forename: Jürgen
authors.initials: J
authors.lastname: Hedderich
authors.forename: Yahya
authors.initials: Y
authors.lastname: Açil
authors.forename: Joerg
authors.initials: J
authors.lastname: Wiltfang
authors.forename: Hendrik
authors.initials: H
authors.lastname: Terheyden
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D001854:Bone Marrow Cells / Q000166:cytology*; D016026:Bone Marrow Transplantation / Q000379:methods*; D018786:Bone Substitutes / Q000737:chemistry; D002130:Calcium Phosphates / Q000737:chemistry; D017886:Durapatite / Q000737:chemistry; D008297:Male; D010012:Osteogenesis / Q000502:physiology; D051381:Rats; D013234:Stem Cells / Q000166:cytology*; D017154:Stromal Cells / Q000166:cytology; D023822:Tissue Engineering / Q000379:methods
chemical_list: D001672:Biocompatible Materials; D018786:Bone Substitutes; D002130:Calcium Phosphates; D017886:Durapatite; C018392:tricalcium phosphate