title: [The assay of transcriptional activation effect of TR3 and deletion mutation in the yeast two hybrid system].
abstract: OBJECTIVE
  To assay the transcriptional activation effect of TR3 and it's deletion mutation in yeast two hybrid system.
  
  
  METHODS
  The total length of TR3 and TR3/delta1-690 gene was amplified by PCR method and cloned into pGBKT7 vector. Bait vector of pGBKT7-TR3 and pGBKT7-TR3/delta1-690 was transformed into AH109 competence yeast. Then self activation of the recombination vector was tested by assay the activity of beta-galactosidae.
  
  
  RESULTS
  The pGBKT7-TR3 and pGBKT7-TR3/AM 690 vector was successfully constructed. The filter paper containing beta-galactosidae didn't changed to blue showed that the reporter gene wasn't activationed.
  
  
  CONCLUSIONS
  TR3 and TR3/delta1-690 hadn't the activity of transcriptional activation.
authors.affiliation: Institute of Health & Environmental Medicine, Tianjin 300050, China. wxxemail@sohu.com
authors.forename: Xin-xing
authors.initials: XX
authors.lastname: Wang
authors.forename: Zhi-hua
authors.initials: ZH
authors.lastname: Yang
authors.forename: Xiao-hua
authors.initials: XH
authors.lastname: Liu
authors.forename: Dong-sheng
authors.initials: DS
authors.lastname: Guo
authors.forename: Ling-jia
authors.initials: LJ
authors.lastname: Qian
mesh_terms: D057105:Nuclear Receptor Subfamily 4, Group A, Member 1 / Q000235:genetics* / Q000502:physiology*; D017384:Sequence Deletion; D015533:Transcriptional Activation / Q000235:genetics*; D020798:Two-Hybrid System Techniques
chemical_list: D057105:Nuclear Receptor Subfamily 4, Group A, Member 1