title: [Effect of acute intra-peritoneal infection on leptin expression levels in peripheral blood and vital organs of rats].
abstract: OBJECTIVE
  To explore the effect of acute intra-peritoneal infection on leptin expression levels in peripheral blood and vital organs, and find out the role leptin plays in acute inflammation.
  
  
  METHODS
  A cecal ligation and perforation model of rats was established, setting groups of sham-operation, intralipid injection, injury, estradiol injection and insulin injection. A rat leptin radioimmunoassay was used to check serum leptin concentrations at 12 h after the injury, and RT-PCR was also used to detect leptin mRNA expressions in adipose tissue, lung and liver.
  
  
  RESULTS
  Compared with serum leptin level of sham-operation group after injury, that of all the other four groups showed no significant difference, while the level of intralipid group was significantly higher than that of injury group and estradiol group. Compared with leptin mRNA expression level of sham-operation group after injury, that of the other four groups had different changes. Leptin mRNA expression of intralipid group was significantly increased in adipose tissue but decreased in lung and liver.
  
  
  CONCLUSIONS
  Leptin expression levels may be affected by the changes of energy metabolism and neuroendocrine function after injury, which suggests a possible protective role for leptin in the recovery of body homeostasis.
authors.affiliation: Research Laboratory of Biochemistry, Basic Medical Institute, General Hospital of P.L.A., Beijing 100853, China.
authors.forename: Ji
authors.initials: J
authors.lastname: Lin
authors.forename: Guang-Tao
authors.initials: GT
authors.lastname: Yan
authors.forename: Lu-Huan
authors.initials: LH
authors.lastname: Wang
mesh_terms: D000818:Animals; D005260:Female; D007249:Inflammation / Q000378:metabolism; D007416:Intestinal Perforation; D020738:Leptin / Q000097:blood / Q000502:physiology*; D008026:Ligation; D008297:Male; D010538:Peritonitis / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D020738:Leptin; D012333:RNA, Messenger