title: [The protective effect of ginsenoside Rg1 on dopaminergic neurons of substantia in the ovariectomized rat model of Parkinson's disease].
abstract: OBJECTIVE
  To investigate the neuroprotective effect of ginsenoside Rg1 on dopaminergic neurons of substantia nigra in ovariectomized rat model of Parkinson's disease and the possible mechanisms.
  
  
  METHODS
  Wistar female rats were ovariectomized and treated with vehicle, ginsenoside Rg1 or 17-beta estradiol intracerebroventricularly in the 6-OHDA induced rat model of Parkinson's disease. Immunohistochemistry was used to detect the tyrosine hydroxylase (TH) immunoreactive neurons and the protein expression of Bcl-2. Perls' iron staining was used to determine the changes of iron in substantia nigra (SN).
  
  
  RESULTS
  910 Rg1 or 17-beta estradiol treatment could ameliorate the rat's rotational behavior induced by apomorphine. 92) Rg1 or 17-beta estradiol treatment could increase TH immunoreactive neurons in the injured side of SN compared to the 6-OHDA group. (3) Iron staining in the injured side of SN was significantly increased comparing with the contralateral side in the 6-OHDA group. Rg1 or 17-beta estradiol treatment could reverse the increase of iron staining. (4) Both Rg1 and 17-beta estradiol treatment could increase Bcl-2 protein expression in the injured side of SN compared to the 6 OHDA group.
  
  
  CONCLUSIONS
  Ginsenoside Rg1 has estrogen-like activities and has neuroprotective effects on the dopaminergic neurons in the 6-OHDA induced ovariectomyzed(OVX) rat model of Parkinson's disease (PD). This effect may be attributed to attenuating iron overload and anti-apoptosis.
authors.affiliation: Deparment of Physiology, Medical College of Qingdao University, Qingdao 266071, China.
authors.forename: Li
authors.initials: L
authors.lastname: Xu
authors.forename: Li-Xing
authors.initials: LX
authors.lastname: Liu
authors.forename: Wen-Fang
authors.initials: WF
authors.lastname: Chen
authors.forename: Jun-Xia
authors.initials: JX
authors.lastname: Xie
authors.forename: Wen-Xiu
authors.initials: WX
authors.lastname: Huang
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D059290:Dopaminergic Neurons / Q000187:drug effects*; D005260:Female; D036145:Ginsenosides / Q000494:pharmacology*; D018696:Neuroprotective Agents / Q000494:pharmacology*; D010052:Ovariectomy; D016627:Oxidopamine; D010302:Parkinson Disease, Secondary / Q000139:chemically induced / Q000503:physiopathology*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013378:Substantia Nigra / Q000187:drug effects* / Q000378:metabolism
chemical_list: D036145:Ginsenosides; D018696:Neuroprotective Agents; D016627:Oxidopamine; C035054:ginsenoside Rg1