title: Construction of a genetic AND gate under a new standard for assembly of genetic parts.
abstract: BACKGROUND
  Appropriate regulation of respective gene expressions is a bottleneck for the realization of artificial biological systems inside living cells. The modification of several promoter sequences is required to achieve appropriate regulation of the systems. However, a time-consuming process is required for the insertion of an operator, a binding site of a protein for gene expression, to the gene regulatory region of a plasmid. Thus, a standardized method for integrating operator sequences to the regulatory region of a plasmid is required.
  
  
  RESULTS
  We developed a standardized method for integrating operator sequences to the regulatory region of a plasmid and constructed a synthetic promoter that functions as a genetic AND gate. By standardizing the regulatory region of a plasmid and the operator parts, we established a platform for modular assembly of the operator parts. Moreover, by assembling two different operator parts on the regulatory region, we constructed a regulatory device with an AND gate function.
  
  
  CONCLUSIONS
  We implemented a new standard to assemble operator parts for construction of functional genetic logic gates. The logic gates at the molecular scale have important implications for reprogramming cellular behavior.
authors.affiliation: Department of Computational Intelligence and Systems Science, Interdisciplinary Graduate School of Science and Engineering, Tokyo Institute of Technology, Midori-ku, Yokohama, Kanagawa, Japan. ayukawa@sb.dis.titech.ac.jp
authors.forename: Shotaro
authors.initials: S
authors.lastname: Ayukawa
authors.forename: Akio
authors.initials: A
authors.lastname: Kobayashi
authors.forename: Yusaku
authors.initials: Y
authors.lastname: Nakashima
authors.forename: Hidemasa
authors.initials: H
authors.lastname: Takagi
authors.forename: Shogo
authors.initials: S
authors.lastname: Hamada
authors.forename: Masahiko
authors.initials: M
authors.lastname: Uchiyama
authors.forename: Katsuyuki
authors.initials: K
authors.lastname: Yugi
authors.forename: Satoshi
authors.initials: S
authors.lastname: Murata
authors.forename: Yasubumi
authors.initials: Y
authors.lastname: Sakakibara
authors.forename: Masami
authors.initials: M
authors.lastname: Hagiya
authors.forename: Masayuki
authors.initials: M
authors.lastname: Yamamura
authors.forename: Daisuke
authors.initials: D
authors.lastname: Kiga
mesh_terms: D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D019295:Computational Biology / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000378:metabolism; D009875:Operator Regions, Genetic / Q000235:genetics; D010957:Plasmids / Q000235:genetics* / Q000592:standards*; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics*
chemical_list: D049452:Green Fluorescent Proteins