title: An aptamer-based chromatographic strip assay for sensitive toxin semi-quantitative detection.
abstract: An aptamer-based chromatographic strip assay method for rapid toxin detection was developed. The aptamer-based strip assay was based on the competition for the aptamer between ochratoxin A and DNA probes. The sensing results indicated that the sensitivity of the aptamer-based strip was better than that of conventional antibody-based strips. The visual limit of detection of the strip for qualitative detection was 1 ng/mL while the LOD for semi-quantitative detection could down to 0.18 ng/mL by using scanning reader. The recoveries of test samples were from 96% to 110%. All detections could be achieved in less than 10 min, indicating that the aptamer-based strip could be a potential useful tool for rapid on-site detections.
authors.affiliation: School of Food Science & Technology, State Key Lab of Food Science & Technology, Jiangnan University, Wuxi 214122, China.
authors.forename: Libing
authors.initials: L
authors.lastname: Wang
authors.forename: Wenwei
authors.initials: W
authors.lastname: Ma
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.forename: Liqiang
authors.initials: L
authors.lastname: Liu
authors.forename: Wei
authors.initials: W
authors.lastname: Ma
authors.forename: Yingyue
authors.initials: Y
authors.lastname: Zhu
authors.forename: Liguang
authors.initials: L
authors.lastname: Xu
authors.forename: Hua
authors.initials: H
authors.lastname: Kuang
authors.forename: Chuanlai
authors.initials: C
authors.lastname: Xu
mesh_terms: D052157:Aptamers, Nucleotide / Q000235:genetics; D001483:Base Sequence; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods* / Q000706:statistics & numerical data; D015342:DNA Probes / Q000235:genetics; D005506:Food Contamination / Q000032:analysis; D006046:Gold; D057230:Limit of Detection; D053768:Metal Nanoparticles; D055616:Microtechnology; D036103:Nanotechnology; D009793:Ochratoxins / Q000032:analysis; D052156:SELEX Aptamer Technique / Q000295:instrumentation* / Q000379:methods* / Q000706:statistics & numerical data; D014118:Toxins, Biological / Q000032:analysis*; D014920:Wine / Q000032:analysis
chemical_list: D052157:Aptamers, Nucleotide; D015342:DNA Probes; D009793:Ochratoxins; D014118:Toxins, Biological; C025589:ochratoxin A; D006046:Gold