title: [The effect of hypoxia preconditioning no binding activity of HIF-1 on the HRE with EPO in the hippocampus of mice].
abstract: OBJECTIVE
  To observe change of binding activity of HIF-1 with erythropoietin (EPO) hypoxia response element (HRE) in the hippocampus of mice preconditioned to hypoxia and explore relationship between the changes and the preconditioning.
  
  
  METHODS
  The hippocampus was removed from mice exposed to hypoxia for 0 run (control group), 1 run (H1 group) and 4 runs(H4 group). Electrophoretic mobility shift assays (EMSA), chromatin immunoprecipitation (ChIP)and real time PCR were used to detect the change of activity of HIF-1 on HRE of EPO.
  
  
  RESULTS
  Both in vitro and in vivo binding tests showed that the HIF-1 DNA-binding activities were increased in group H1 and markedly increased in group H4.
  
  
  CONCLUSIONS
  The increase of HIF-1 and HRE of EPO binding activities is thought be involved in hypoxic preconditioning.
authors.affiliation: Department of Neurobiology, Capital University of Medical Sciences, 100054 Beijing, China.
authors.forename: Guo
authors.initials: G
authors.lastname: Shao
authors.forename: Wei-Hua
authors.initials: WH
authors.lastname: Zhou
authors.forename: Cui-Ying
authors.initials: CY
authors.lastname: Gao
authors.forename: Ran
authors.initials: R
authors.lastname: Zhang
authors.forename: Guo-Wei
authors.initials: GW
authors.lastname: Lu
mesh_terms: D000818:Animals; D004921:Erythropoietin / Q000378:metabolism*; D006624:Hippocampus / Q000378:metabolism*; D000860:Hypoxia / Q000378:metabolism*; D051793:Hypoxia-Inducible Factor 1 / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D020218:Response Elements
chemical_list: D051793:Hypoxia-Inducible Factor 1; D004921:Erythropoietin