title: CYP450 biosensors based on screen-printed carbon electrodes for the determination of cocaine.
abstract: A new electrochemical method has been described and characterized for the determination of cocaine using screen-printed biosensors. The enzyme cytochrome P450 was covalently attached to screen-printed carbon electrodes. Experimental design methodology has been performed to optimize the pH and the applied potential, both variables that have an influence on the chronoamperometric determination of the drug. This method showed a reproducibility of 3.56% (n=4) related to the slopes of the calibration curves performed in the range from 19 up to 166nM. It has been probed the used of this kind of biosensors in the determination of cocaine in street samples, with an average capability of detection of 23.05±3.53nM (n=3, α=β=0.05).
authors.affiliation: Analytical Chemistry Department, Faculty of Sciences, University of Burgos, Pza. Misael Bañuelos s/n, 09001 Burgos, Spain.
authors.forename: Laura
authors.initials: L
authors.lastname: Asturias-Arribas
authors.forename: M Asunción
authors.initials: MA
authors.lastname: Alonso-Lomillo
authors.forename: Olga
authors.initials: O
authors.lastname: Domínguez-Renedo
authors.forename: M Julia
authors.initials: MJ
authors.lastname: Arcos-Martínez
mesh_terms: D001189:Aryl Hydrocarbon Hydroxylases / Q000378:metabolism*; D015374:Biosensing Techniques / Q000379:methods*; D002244:Carbon / Q000737:chemistry; D016578:Crack Cocaine / Q000032:analysis* / Q000378:metabolism; D000072467:Cytochrome P450 Family 2; D004563:Electrochemistry / Q000379:methods; D004800:Enzymes, Immobilized / Q000378:metabolism; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D016578:Crack Cocaine; D004800:Enzymes, Immobilized; D002244:Carbon; D001189:Aryl Hydrocarbon Hydroxylases; D000072467:Cytochrome P450 Family 2; C465241:cytochrome P-450 CYP2B4 (rabbit)