title: Trichostatin A-treated eight-cell bovine embryos had increased histone acetylation and gene expression, with increased cell numbers at the blastocyst stage.
abstract: The objective was to determine the effects of trichostatin A (TSA), a potent histone deacetylase inhibitor, on eight-cell bovine embryos. That treatment increased histone acetylation was confirmed by immunostaining with anti-AcH4K5 and AcH4K8 antibodies. Embryos treated with TSA (100 nM) for various intervals (4, 8, and 12 h) developed to the blastocyst stage as frequently as untreated embryos (average development rate, 49.5%). Treatment with TSA for 12 h increased (P < 0.05) the numbers of inner cell mass (ICM) cells and total cells (TC), as well as the ICM/TC ratio in the blastocyst, but the number of cells in the trophectoderm decreased (P < 0.05). Treated embryos had increased relative abundance (RA) of OCT3/4 and E-CADHERIN mRNA relative to controls at the morula stage (P < 0.05), however, the RA of CDX2 mRNA was unchanged. In conclusion, TSA-treated eight-cell stage embryos had increased histone acetylation and gene expression, which increased ICM and TC numbers and the ICM/TC ratio, but significantly decreased the number of cells in the trophectoderm of resulting blastocysts.
authors.affiliation: Tokyo University of Agriculture, Funako 1737, Atsugi City 243-0024, Japan.
authors.forename: Shogo
authors.initials: S
authors.lastname: Shiratsuki
authors.forename: Hisataka
authors.initials: H
authors.lastname: Iwata
authors.forename: Koji
authors.initials: K
authors.lastname: Kimura
authors.forename: Takeshi
authors.initials: T
authors.lastname: Kuge
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Monji
authors.forename: Takehito
authors.initials: T
authors.lastname: Kuwayama
mesh_terms: D000107:Acetylation; D000818:Animals; D001755:Blastocyst / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D015820:Cadherins / Q000235:genetics; D002417:Cattle / Q000196:embryology*; D002452:Cell Count; D015870:Gene Expression / Q000187:drug effects*; D056572:Histone Deacetylase Inhibitors / Q000494:pharmacology*; D006657:Histones / Q000378:metabolism*; D018398:Homeodomain Proteins / Q000235:genetics; D006877:Hydroxamic Acids / Q000494:pharmacology*; D050814:Octamer Transcription Factor-3 / Q000235:genetics
chemical_list: D015820:Cadherins; D056572:Histone Deacetylase Inhibitors; D006657:Histones; D018398:Homeodomain Proteins; D006877:Hydroxamic Acids; D050814:Octamer Transcription Factor-3; C012589:trichostatin A