title: siRNA-mediated knockdown of the melanocortin 2 receptor accessory protein 2 (MRAP2) gene confers resistance to methylmercury on HEK293 cells.
abstract: Methylmercury is a well-known environmental pollutant that causes serious disorders of the central nervous system as well as a range of other symptoms. We employed small interfering RNA (siRNA) to search for factors in ligand-dependent signal transduction pathways that may be involved in the development of methylmercury toxicity. Melanocortin 2 receptor accessory protein 2 (MRAP2) is involved in the melanocortin pathway. Using siRNA, we found that decreased expression of MRAP2 conferred strong methylmercury resistance in HEK293 cells.
authors.affiliation: Laboratory of Molecular and Biochemical Toxicology, Graduate School of Pharmaceutical Sciences, Tohoku University, Miyagi, Japan.
authors.forename: Gi-Wook
authors.initials: GW
authors.lastname: Hwang
authors.forename: Seong-Eun
authors.initials: SE
authors.lastname: Oh
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Takahashi
authors.forename: Jin-Yong
authors.initials: JY
authors.lastname: Lee
authors.forename: Akira
authors.initials: A
authors.lastname: Naganuma
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins / Q000037:antagonists & inhibitors / Q000235:genetics*; D004351:Drug Resistance / Q000235:genetics*; D004785:Environmental Pollutants / Q000633:toxicity*; D057809:HEK293 Cells; D006801:Humans; D008024:Ligands; D008767:Methylmercury Compounds / Q000633:toxicity*; D034741:RNA, Small Interfering / Q000235:genetics*; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; D004785:Environmental Pollutants; D008024:Ligands; C551627:MRAP2 protein, human; D008767:Methylmercury Compounds; D034741:RNA, Small Interfering; C004925:methylmercuric chloride