title: Oxidized cellulose binding to allergens with a carbohydrate-binding module attenuates allergic reactions.
abstract: Grass and mite allergens are of the main causes of allergy and asthma. A carbohydrate-binding module (CBM) represents a common motif to groups I (β-expansin) and II/III (expansin-like) grass allergens and is suggested to mediate allergen-IgE binding. House dust mite group II allergen (Der p 2 and Der f 2) structures bear strong similarity to expansin's CBM, suggesting their ability to bind carbohydrates. Thus, this study proposes the design of a carbohydrate-based treatment in which allergen binding to carbohydrate particles will promote allergen airway clearance and prevent allergic reactions. The aim of the study was to identify a polysaccharide with high allergen-binding capacities and to explore its ability to prevent allergy. Oxidized cellulose (OC) demonstrated allergen-binding capacities toward grass and mite allergens that surpassed those of any other polysaccharide examined in this study. Furthermore, inhalant preparations of OC microparticles attenuated allergic lung inflammation in rye grass-sensitized Brown Norway rats and OVA-sensitized BALB/c mice. Fluorescently labeled OC efficiently cleared from the mouse airways and body organs. Moreover, long-term administration of OC inhalant to Wistar rats did not result in toxicity. In conclusion, many allergens, such as grass and dust mite, contain a common CBM motif. OC demonstrates a strong and relatively specific allergen-binding capacity to CBM-containing allergens. OC's ability to attenuate allergic inflammation, together with its documented safety record, forms a firm basis for its application as an alternative treatment for prevention and relief of allergy and asthma.
authors.affiliation: Robert H. Smith Faculty of Agriculture, Food, and Environment, Hebrew University of Jerusalem, Jerusalem, Israel.
authors.forename: Nir
authors.initials: N
authors.lastname: Shani
authors.forename: Ziv
authors.initials: Z
authors.lastname: Shani
authors.forename: Oded
authors.initials: O
authors.lastname: Shoseyov
authors.forename: Rufayda
authors.initials: R
authors.lastname: Mruwat
authors.forename: David
authors.initials: D
authors.lastname: Shoseyov
mesh_terms: D000485:Allergens / Q000276:immunology / Q000378:metabolism*; D000818:Animals; D039741:Antigens, Dermatophagoides / Q000276:immunology / Q000378:metabolism; D060829:Arthropod Proteins; D050260:Carbohydrate Metabolism / Q000276:immunology*; D002482:Cellulose / Q000008:administration & dosage / Q000276:immunology / Q000378:metabolism*; D003546:Cysteine Endopeptidases; D005260:Female; D008129:Lolium / Q000276:immunology; D008168:Lung / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010084:Oxidation-Reduction; D011058:Pollen / Q000276:immunology / Q000378:metabolism*; D011485:Protein Binding / Q000276:immunology; D039981:Pyroglyphidae / Q000276:immunology / Q000378:metabolism; D051381:Rats; D011914:Rats, Inbred BN; D017208:Rats, Wistar; D012130:Respiratory Hypersensitivity / Q000276:immunology* / Q000378:metabolism* / Q000473:pathology
chemical_list: D000485:Allergens; D039741:Antigens, Dermatophagoides; D060829:Arthropod Proteins; C451163:Dermatophagoides pteronyssinus antigen p 2; D002482:Cellulose; D003546:Cysteine Endopeptidases; C451162:Dermatophagoides pteronyssinus antigen p 1; C109691:microcrystalline cellulose