title: Platelet-released supernatant induces osteoblastic differentiation of human mesenchymal stem cells: potential role of BMP-2.
abstract: Platelet-rich preparations have recently gained popularity in maxillofacial and dental surgery, but their beneficial effect is still under debate. Furthermore, very little is known about the effect of platelet preparations at the cellular level, and the underlying mechanisms. In this study, we tested the effect of platelet-released supernatant (PRS) on human mesenchymal stem cell (MSC) differentiation towards an osteoblastic phenotype in vitro. Cultures of MSC were supplemented with PRS and typical osteoblastic markers were assessed at up to 28 days post-confluence. PRS showed an osteoinductive effect on MSC, as shown by an increased expression of typical osteoblastic marker genes such as collagen Ialpha1, bone sialoprotein II, BMP-2 and MMP-13, as well as by increased ⁴⁵Ca²+ incorporation. Our results suggest that the effect of PRS on human MSC could be at least partially mediated by BMP-2. Activated autologous PRS could therefore provide an alternative to agents like recombinant bone growth factors by increasing osteoblastic differentiation of bone precursor cells at bone repair sites, although further studies are needed to fully support our observations.
authors.affiliation: AO Research Institute, CH-7270 Davos, Switzerland.
authors.forename: S
authors.initials: S
authors.lastname: Verrier
authors.forename: T R
authors.initials: TR
authors.lastname: Meury
authors.forename: L
authors.initials: L
authors.lastname: Kupcsik
authors.forename: P
authors.initials: P
authors.lastname: Heini
authors.forename: T
authors.initials: T
authors.lastname: Stoll
authors.forename: M
authors.initials: M
authors.lastname: Alini
mesh_terms: D000328:Adult; D000368:Aged; D001685:Biological Factors / Q000097:blood*; D001792:Blood Platelets / Q000378:metabolism*; D055396:Bone Morphogenetic Protein 2 / Q000096:biosynthesis*; D002118:Calcium / Q000378:metabolism; D002454:Cell Differentiation; D002478:Cells, Cultured; D005260:Female; D020869:Gene Expression Profiling; D005819:Genetic Markers; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology; D008875:Middle Aged; D010006:Osteoblasts / Q000166:cytology*; D013347:Subcellular Fractions / Q000378:metabolism
chemical_list: C525718:BMP2 protein, human; D001685:Biological Factors; D055396:Bone Morphogenetic Protein 2; D005819:Genetic Markers; D002118:Calcium