title: [Effect of ischemic preconditioning on lung injury induced by ischemia/reperfusion in the hind limbs of rats].
abstract: OBJECTIVE
  To study the effect of ischemic preconditioning on lung injury following ischemia/reperfusion (I/R) in the hind limbs of rats.
  
  
  METHODS
  Wistar rats were randomly divided into four groups (n=8): control group,limbs ischemia/reperfusion (LI/R) group, ischemia preconditioning (IPC) group and L-NAME group. At the end of the experiment, blood/gas analysis and the contents of serum MDA, NO, ET and lung tissue MDA, NO, ET, MPO were measured. Meanwhile, lung index and W/D) of lung were measured.
  
  
  RESULTS
  After the rats' hind limbs suffered ischemia/reperfusion, the level of PaO2 decreased and the values of W/D, LI, MPO of the lung issure and MDA, NO, ET of plasma and lung all increased significantly in the LI/R group; but the ratio of NO/ET decreased. Compared with LI/R group, the contents of NO and ratio of NO/ET increased but other parameters decreased in the IPC group. Compared with IPC group, the contents of NO and ratio of NO/ET decreased, but other parameters increased in the L-NAME group.
  
  
  CONCLUSIONS
  The IPC can attenuate lung injury following IR in the hind limbs of rats, which may correlated with the increase of NO.
authors.affiliation: North China Coal Medical College, Tangshan 063000, China.
authors.forename: Hong-bo
authors.initials: HB
authors.lastname: Gao
authors.forename: Lin-jing
authors.initials: LJ
authors.lastname: Zhao
authors.forename: Shu-yun
authors.initials: SY
authors.lastname: Dong
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
authors.forename: Na
authors.initials: N
authors.lastname: Zhang
authors.forename: Yin-huan
authors.initials: YH
authors.lastname: Wang
authors.forename: Lian-yuan
authors.initials: LY
authors.lastname: Zhang
mesh_terms: D055371:Acute Lung Injury / Q000517:prevention & control*; D000818:Animals; D005121:Extremities / Q000098:blood supply*; D007511:Ischemia / Q000503:physiopathology; D019194:Ischemic Preconditioning / Q000379:methods*; D008168:Lung / Q000098:blood supply; D008297:Male; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000378:metabolism / Q000517:prevention & control*