title: [Simultaneous determination of five active components in compound preparation of Dangguishuan by high-performance liquid chromatography].
abstract: OBJECTIVE
  To develop a HPLC method for determination of matrine, oxymatrine; ferulic acid, L-shikonin and beta, beta-dimethylacrylshikonin in compound preparatioti of Dangguishuan.
  
  
  METHODS
  The chromatogtaphic separation was performed on a Kromasil ODS C18 column (4.6 mm x 250 mm, 5 microm) maintaining at 30 degrees C during the whole process. The mobile phase consisted of methanol and 0.1% triethylamine aqueous solution (adjusted with phosphoric acid, pH 3) at a flow rate of 1.0 mL x min(-1). The detection wavelength was set at 220 nm for matririne and oxymatrine, 316 nm for ferulic acid, 516 nm for L-shikonin and beta, beta-dimethylacrylshikonin, respectively.
  
  
  RESULTS
  All the compounds showed good linearity (r > 0.9996) in the range of the test concentrations, and the average recoveries of the method is betwuen 96.92% and 102.22%, RSD < 3.1%.
  
  
  CONCLUSIONS
  The method is proved to be credible, sensitive, accurate and repeatable. It can be applied to determine of matrine, oxymatrine, ferulic acid, L-shikonin and beta, beta-dimethylacrylshikonin in compound preparation of Dangguishuan simultaneously, and provide a basal method of quality control to this preparation and other relative preparations.
authors.affiliation: School of Pharmaceutics, Lanzhou University, Lanzhou 730000, China.
authors.forename: Yingyan
authors.initials: Y
authors.lastname: Bi
authors.forename: Fangdi
authors.initials: F
authors.lastname: Hu
authors.forename: Shilan
authors.initials: S
authors.lastname: Feng
authors.forename: Yikai
authors.initials: Y
authors.lastname: Shi
authors.forename: Chunming
authors.initials: C
authors.lastname: Wang
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Ma
mesh_terms: D000470:Alkaloids / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003373:Coumaric Acids / Q000032:analysis; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D009285:Naphthoquinones / Q000032:analysis; D011807:Quinolizines / Q000032:analysis; D000093842:Matrines
chemical_list: D000470:Alkaloids; D003373:Coumaric Acids; D004365:Drugs, Chinese Herbal; D009285:Naphthoquinones; D011807:Quinolizines; C016101:shikonin; C037573:oxymatrine; C004999:ferulic acid; D000093842:Matrines