title: [Effects and relationship between NO and HIF-1alpha in rats with pulmonary hypertension induced by hypoxia].
abstract: OBJECTIVE
  To investigate the expression of hypoxia-inducible factor-1 alpha (HIF-1alpha) in rats with chronic pulmonary hypertension induced by hypoxia and hypercapnia and its relationship with nitric oxide(NO).
  
  
  METHODS
  Fourty male Sprague-Dawley rats were randomly divided into four groups, normal control group (NC), hypoxia-hypercapnia group (HH), hypoxia - hypercapnia + L-arginine liposome group(HP) and hypoxia-hypercapnia+ N-nitro-L-arginine methylester group (HM). Colorimetric analysis, immunohistochemistry and in situ hybridization were used for detection of NO, HIF-1alpha and constitutive nitric oxide synthase (ecNOS).
  
  
  RESULTS
  (1) The mean pulmonary arterial pressure (mPAP) and the weight ratio of right ventricular to left ventricle plus septum (RV/(LV + S)) of HH group were higher than those of NC group (P < 0.05), HP group much lower than HH group (P < 0.01), mPAP of HM higher than HH group ( P < 0.05). 2)0 Contents of NO in plasma and pulmonary tissue homogenates of HH group were much lower than those of NC group (P < 0.01), HP group higher than HH group (P < 0.01). There were no difference between HM group and HH group. (Expression of HIF-1alpha and HIF-1alpha mRNA in pulmonary arterioles of HH group were significantly higher than those of NC group( P < 0.01), HP group lower than HH group (P < 0.01) ,HM group higher than HH group (P < 0.01); Whereas expression of ecNOS and ecNOS mRNA in pulmonary arterioles of HH were lower than those of NC group( P < 0.05, IP group higher than HH group (P < 0.01), HM group lower than HH group (P < 0.05).
  
  
  CONCLUSIONS
  HIF-1alpha is involved in the pathogenesis of chronic pulmonary hypertension induced by hypoxia and hypercapnia. The protective function of NO in the pathogenesis might be partly depended on its effects on the expression/activity of HIF-1alpha in lung.
authors.affiliation: Institute of Cor Pulmonale, Wenzhou Medical College, Wenzhou 325027, China.
authors.forename: Jian-Feng
authors.initials: JF
authors.lastname: Luo
authors.forename: Xiao-Mai
authors.initials: XM
authors.lastname: Wu
authors.forename: Xiao-Fang
authors.initials: XF
authors.lastname: Fan
authors.forename: Liang-Gang
authors.initials: LG
authors.lastname: Hu
authors.forename: Hong
authors.initials: H
authors.lastname: Huang
authors.forename: Shan-Shan
authors.initials: SS
authors.lastname: Jia
authors.forename: Sun Zhong
authors.initials: SZ
authors.lastname: Mao
authors.forename: Yong-Sheng
authors.initials: YS
authors.lastname: Gong
mesh_terms: D000818:Animals; D006976:Hypertension, Pulmonary / Q000209:etiology / Q000378:metabolism*; D000860:Hypoxia; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism*; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D012333:RNA, Messenger; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase