title: [Actions of Syn-1A on blocking the activation of K(ATP) channel induced by acidic pH].
abstract: OBJECTIVE
  To investigate the action and mechanism of Syn-1A in reversing the activation of K(ATP) channel induced by weak acidic pH.
  
  
  METHODS
  The patches excised from Kir6.2/SUR2A expressing HEK-293 cells were used to establish inside-out configuration. To examine the actions of weak acidic pH in activation of the channel and the reverse action of Syn-1A on it, the inside-out patches were continuously perfused with the solution of pH from 7.4, 7.0, 6.8, 6.5 to 6.0 with or without Syn-1A. In vitro binding was employed to study the influence of different pH to the binding of Syn-1A to SUR2A subunit.
  
  
  RESULTS
  Syn-1A blocked pH 6.5, 6.8 and 7.0 induced activation of the channel, and Syn-1A binding to SUR2A were increased by reducing pH from 7.4 to 6.0.
  
  
  CONCLUSIONS
  Syn-1A would assert some inhibition of the KATP channels, which might temper the fluctuation of acidic pH-induced K(ATP) channel opening that could induce fatal re-entrant arrhythmias.
authors.affiliation: School of Public Health and Family Medicine, Capital Medical University, Beijing 100069, China.
authors.forename: Jun-Li
authors.initials: JL
authors.lastname: Ma
authors.forename: Guo-Qing
authors.initials: GQ
authors.lastname: Zhuang
authors.forename: Yan-Xia
authors.initials: YX
authors.lastname: Luo
authors.forename: Fen
authors.initials: F
authors.lastname: Liu
authors.forename: Yan
authors.initials: Y
authors.lastname: He
mesh_terms: D057809:HEK293 Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D054086:KATP Channels / Q000378:metabolism*; D018408:Patch-Clamp Techniques; D015221:Potassium Channels / Q000378:metabolism*; D024661:Potassium Channels, Inwardly Rectifying / Q000378:metabolism; D050827:Syntaxin 1 / Q000494:pharmacology*
chemical_list: D054086:KATP Channels; D015221:Potassium Channels; D024661:Potassium Channels, Inwardly Rectifying; D050827:Syntaxin 1