title: [Expression of a human FL eukaryotic expressing plasmid mediated by lipofectamine in HFCL cells].
abstract: OBJECTIVE
  Bone marrow stromal cell line-HFCL were transfected with the recombinant eukaryotic expressing vector-pIRESlneo/hFL by using liposome-mediated gene transfer method and get a stable expression.
  
  
  METHODS
  HFCL cells were transfected with the recombinant eukaryotic expressing vector-pIRESlneo/hFL by using liposome lipofectamine. Integration of hFL in the genome, transcription of hFL mRNA and expression of hFL protein in the transfected HFCL cells were assayed by Southern blot, Northern blot, Western blot and ELISA, the experiment of the human umbilical blood CD34+ cell multiplication.
  
  
  RESULTS
  hFL cDNA was integrated into HFCL genome successfully, hFL mRNA was transcripted, hFL protein was expressed with (60.3 +/- 0.1) ng. 10(6) cell(-1) x d(-1) and the experiment of the human umbilical blood CD34+ cell multiplication shows that hFL has obvious biological activity in the supernatant.
  
  
  CONCLUSIONS
  The recombinant plasmid is proved to be stably expressed in HFCL cells and obvious biological activity of hFL was detectable in the supernatant of the transfected cells.
authors.affiliation: Department of Hematology & Oncology, First Clinical College, Norman Bethune University of Medical Sciences, Changchun 130021, China.
authors.forename: L J
authors.initials: LJ
authors.lastname: Ma
authors.forename: G J
authors.initials: GJ
authors.lastname: Wang
authors.forename: L
authors.initials: L
authors.lastname: Li
authors.forename: X
authors.initials: X
authors.lastname: Hong
authors.forename: X T
authors.initials: XT
authors.lastname: Pei
mesh_terms: D001854:Bone Marrow Cells / Q000378:metabolism*; D002460:Cell Line; D005822:Genetic Vectors; D006801:Humans; D008055:Lipids; D008081:Liposomes; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D010957:Plasmids / Q000235:genetics; D017154:Stromal Cells / Q000378:metabolism*; D014162:Transfection
chemical_list: D008055:Lipids; C086724:Lipofectamine; D008081:Liposomes; D008565:Membrane Proteins