title: Value of centrifugated liquid-based cytology by Papanicolaou and May-Grünwald in oral epithelial cells.
abstract: For many years, liquid-based cytology (LBC) has been developed for cervical cancer screening and not oral cancer, as it requires automated devices. The aim of this study was to compare the utility of centrifugated CLBC preparation with that of direct preparation in oral lesions, by Papanicolaou (Pap) and May Grünwald-Giemsa's (MGG) methods. A total of 100 consecutive cases of oral lesions were investigated. We compared the results obtained by the CLBC performed by cytocentrifugation with those obtained by direct smear applying Pap and MGG methods. The comparison between CLBC and direct smears was based on the thickening or adequacy of the smear, distribution of cells and staining quality. All smears in CLBC and direct preparation were found adequate. For thickness of the smear, 40% and 42% were excellent, 33% and 30% were good, and 27% and 28% were acceptable by LBC and direct preparation, respectively. For the distribution of cells and scantiness of background elements, 92 (92%) smears of the CLBC have revealed clear, well distributed smears, compared to 70 (70%) of those in direct preparation. For the staining quality with the Pap method, 39% and 69% were excellent staining quality, 25% and 20% were good, and 36% and 11% were acceptable for CLBC and direct preparation, respectively. In MGG method, 9% and 22% were excellent staining quality, 23% and 36% were good and 68% and 43% were acceptable for CLBC and direct preparation respectively. CLBC performed by cytocentrifugation is inexpensive, and reduces inadequate smears and background staining.
authors.affiliation: Department of Histopathology and Cytology, Faculty of Medical Laboratory Sciences, Khartoum, Sudan;
authors.forename: Hussain Gadelkarim
authors.initials: HG
authors.lastname: Ahmed
authors.forename: Ali Mahmmoud
authors.initials: AM
authors.lastname: Edris
authors.forename: Eneel Ahmed
authors.initials: EA
authors.lastname: Mohmed
authors.forename: Mohammed Omer M
authors.initials: MO
authors.lastname: Hussein