title: Physicochemical characterization of protein-loaded pectin-chitosan nanoparticles prepared by polyelectrolyte complexation.
abstract: Recent advances in nanotechnology applied to proteins are directed towards safer and simpler methods of preparation, using naturally occurring polymers such as alginate, pectin and chitosan. In this study, pectin-chitosan nanoparticles (NPs) were designed by the mild process of polyelectrolyte complexation, which occurs at room temperature without using sonication or organic solvents. NPs with a mean diameter between 300 and 400 nm and 45 to 86% protein association efficiency were obtained by varying the pectin:chitosan mass ratio and initial protein concentration. A prolonged release profile without burst effect of investigated ovalbumin from pectin-chitosan NPs was determined.
authors.affiliation: Faculty of Pharmacy, University of Ljubljana, Ljubljana, Slovenia. ahlinp@ffa.uni-lj.si
authors.forename: P Ahlin
authors.initials: PA
authors.lastname: Grabnar
authors.forename: J
authors.initials: J
authors.lastname: Kristl
mesh_terms: D048271:Chitosan / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D004573:Electrolytes / Q000737:chemistry*; D005079:Excipients; D019344:Lactic Acid; D053758:Nanoparticles / Q000737:chemistry*; D010047:Ovalbumin / Q000737:chemistry; D010368:Pectins / Q000737:chemistry*; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers; D011506:Proteins / Q000008:administration & dosage / Q000737:chemistry*; D012997:Solvents; D014465:Ultrasonics
chemical_list: D004573:Electrolytes; D005079:Excipients; D011108:Polymers; D011506:Proteins; D012997:Solvents; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D010368:Pectins; D010047:Ovalbumin; D048271:Chitosan