title: Quantification of ultra-trace molybdenum using 4-amino-5-hydroxynaphthalene-2,7-disulfonic acid monosodium salt as a chromogenic probe.
abstract: A simple, ultrasensitive, nonextractive spectrophotometric method has been developed for the assay of Mo(VI), which involves Mo-catalyzed oxidation of 4-amino-5-hydroxynaphthalene-2,7-disulfonic acid monosodium salt (AHNDSA) by H(2)O(2) in acetic acid/sodium acetate buffer yielding an intense pink colored product with λ(max) of 540 nm. Beer's law is obeyed in the range of 10-240 ng/ml with molar absorptivity of 3.0137×10(5)L mol(-1)cm(-1). The LOD and LOQ were found to be 0.7696 and 2.565 ng/ml, respectively. The applicability of the method toward water and biological samples was tested and statistically compared with a reference method.
authors.affiliation: Department of Studies in Chemistry, University of Mysore, Manasagangotri, Mysore 570 006, India. profpn58@yahoo.com
authors.forename: Padmarajaiah
authors.initials: P
authors.lastname: Nagaraja
authors.forename: Honnur
authors.initials: H
authors.lastname: Krishna
authors.forename: Anantharaman
authors.initials: A
authors.lastname: Shivakumar
authors.forename: Anthonydas Robert
authors.initials: AR
authors.lastname: Paulas
authors.forename: Dinesh
authors.initials: D
authors.lastname: Rangappa
mesh_terms: D002384:Catalysis; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D008982:Molybdenum / Q000097:blood*; D009282:Naphthalenesulfonates / Q000737:chemistry*; D010084:Oxidation-Reduction; D012492:Salts / Q000737:chemistry; D013053:Spectrophotometry / Q000379:methods*
chemical_list: C550473:4-amino-5-hydroxynaphthalene-2,7-disulfonic acid; D005456:Fluorescent Dyes; D009282:Naphthalenesulfonates; D012492:Salts; D008982:Molybdenum