title: Preparative isotachophoresis with surface enhanced Raman scattering as a promising tool for clinical samples analysis.
abstract: A surface enhanced Raman scattering (SERS) spectrometry is an interesting alternative for a rapid molecular recognition of analytes at very low concentration levels. The hyphenation of this technique with advanced separation methods enhances its potential as a detection technique. Until now, it has been hyphenated mainly with common chromatographic and electrophoretic techniques. This work demonstrates for a first time a power of preparative isotachophoresis-surface enhanced Raman scattering spectrometry (pITP-SERS) combination on the analysis of model analyte (buserelin) in a complex biological sample (urine). An off-line identification of target analyte was performed using a comparison of Raman spectra of buserelin standard with spectra obtained by the analyses of the fractions from preparative isotachophoretic runs. SERS determination of buserelin was based on the method of standard addition to minimize the matrix effects. The linearity of developed method was obtained in the concentration range from 0.2 to 1.5 nmol L(-1) with coefficient of determination 0.991. The calculated limit of detection is in tens of pico mols per liter.
authors.affiliation: Department of Analytical Chemistry, Faculty of Natural Sciences, Palacký University, 17. Listopadu 12, CZ-77146 Olomouc, Czech Republic.
authors.forename: Václav
authors.initials: V
authors.lastname: Ranc
authors.forename: Andrea
authors.initials: A
authors.lastname: Staňová
authors.forename: Jozef
authors.initials: J
authors.lastname: Marák
authors.forename: Vítězslav
authors.initials: V
authors.lastname: Maier
authors.forename: Juraj
authors.initials: J
authors.lastname: Sevčík
authors.forename: Dušan
authors.initials: D
authors.lastname: Kaniansky
mesh_terms: D002064:Buserelin / Q000652:urine; D006801:Humans; D057625:Isotachophoresis / Q000379:methods*; D053768:Metal Nanoparticles / Q000737:chemistry; D012680:Sensitivity and Specificity; D012834:Silver / Q000737:chemistry; D013059:Spectrum Analysis, Raman / Q000379:methods*
chemical_list: D012834:Silver; D002064:Buserelin