title: Injury-induced activation of ERK 1/2 in the sciatic nerve of healthy and diabetic rats.
abstract: Phosphorylation of extracellular-signal-regulated kinase 1/2 (p-ERK 1/2) was investigated by immunohistochemistry at 30 min, 1 h, and 48 h after nerve transection in the sciatic nerve of healthy and diabetic [streptozotocin (STZ)-induced diabetes mellitus and BioBreeding (BB; i.e. DR.lyp/lyp or BBDP)] rats. Transection injury increased the intensity of p-ERK 1/2 in nerve stumps at all time points. Staining was confined to Schwann cells with occasional faint staining in single axons. In diabetic rats, a lower intensity of p-ERK 1/2 was found at 1 and 48 h in the distal and proximal nerve stumps compared with healthy rats. STZ-induced diabetic rats were not different from BB rats. p-ERK 1/2 is activated differentially in Schwann cells after nerve injury in diabetic rats, whereas activation in STZ-induced diabetic rats did not differ from BB rats.
authors.affiliation: Department of Clinical Sciences Malmö-Hand Surgery, Skåne University Hospital, Malmö, Sweden.
authors.forename: Lena
authors.initials: L
authors.lastname: Stenberg
authors.forename: Martin
authors.initials: M
authors.lastname: Kanje
authors.forename: Lisa
authors.initials: L
authors.lastname: Mårtensson
authors.forename: Lars B
authors.initials: LB
authors.lastname: Dahlin
mesh_terms: D000818:Animals; D001369:Axons / Q000201:enzymology; D003921:Diabetes Mellitus, Experimental / Q000201:enzymology*; D005260:Female; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism*; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism*; D010766:Phosphorylation; D051381:Rats; D017208:Rats, Wistar; D012583:Schwann Cells / Q000201:enzymology*; D012584:Sciatic Nerve / Q000201:enzymology* / Q000293:injuries*; D013997:Time Factors
chemical_list: D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3