title: Sixteen polymorphic simple sequence repeat markers from expressed sequence tags of the Chinese Mitten Crab Eriocheir sinensis.
abstract: The Chinese mitten crab (Eriocheir sinensis) is an economically important aquaculture species in China. In this study, we developed and evaluated simple sequence repeat markers from expressed sequence tags of E. sinensis. Among the 40 wild E. sinensis individuals tested, 16 loci were polymorphic. The number of alleles per locus ranged from two to ten. The observed heterozygosity ranged from 0.0667 to 0.9667, whereas the expected heterozygosity ranged from 0.0661 to 0.9051. These markers have the potential for use in genetic studies of population structure and intraspecific variation in E. sinensis.
authors.affiliation: Liaoning Key Laboratory of Marine Fishery Molecular Biology, Liaoning Ocean and Fishery Science Institute, 50 Heishijiao St, Dalian, Liaoning 116023, China; E-Mail: xiangganggao@hotmail.com (X.-G.G.); oceanlhj@163.com (H.-J.L.); yfli2008@yahoo.cn (Y.-F.L.); cnliu51@tom.com (W.-D.L.).
authors.forename: Xiang-Gang
authors.initials: XG
authors.lastname: Gao
authors.forename: Hong-Jun
authors.initials: HJ
authors.lastname: Li
authors.forename: Yun-Feng
authors.initials: YF
authors.lastname: Li
authors.forename: Li-Jun
authors.initials: LJ
authors.lastname: Sui
authors.forename: Bao
authors.initials: B
authors.lastname: Zhu
authors.forename: Yu
authors.initials: Y
authors.lastname: Liang
authors.forename: Wei-Dong
authors.initials: WD
authors.lastname: Liu
authors.forename: Chong-Bo
authors.initials: CB
authors.lastname: He
mesh_terms: D000818:Animals; D003386:Brachyura / Q000235:genetics*; D020224:Expressed Sequence Tags; D018895:Microsatellite Repeats; D011110:Polymorphism, Genetic