title: [Protective effects of adenovirus-mediated hepatocyte growth factor on injury of rat cortex neurons].
abstract: OBJECTIVE
  To investigate the protective effects of adenovirus-mediated hepatocyte growth factor (Ad-HGF) on injury of rat cortex neurons induced by in vitro serum-free culture.
  
  
  METHODS
  Flow cytometry was used to assay the transfection rate of rat cortex neurons infected by adenovirus-mediated green fluorescent protein(Ad-GFP) at different multiplicity of infection (MOI) to find out the best MOI in experiment. ELISA was used to elucidate the expression patterns of cortex neuron. Neutral red stain and PI-Hoechst 33342 double stain were used to compare the viability of cortex neurons, which were cultured in serum-free medium for 6 h, 12 h, 24 h and 48 h respectively, among the Ad-HGF transfected group, the Ad-GFP transfected group and the control group.
  
  
  RESULTS
  It was found that when MOI was 50 PFU per cell, a transfection rate as high as 99.3% was maintained and Ad-HGF was able to express in cortex neurons effectively and persistently. In addition, the death rate and apoptotic rate of cortex neurons (infected 2 hours after seeding) cultured in serum-free medium for 12 h in Ad-HGF transfected group was significantly lower than that in both the Ad-GFP group and the control group (P < 0.05).
  
  
  CONCLUSIONS
  Ad-HGF plays a protective role against in vitro serum-free culture induced injury on rat cortex neurons infected 2 hours after seeding. Though its effects on rat cortex neurons infected 5 days after seeding are not so remarkable, Ad-HGF also has the potential to protect cortex neurons from serum-free culture induced injury.
authors.affiliation: Department of Biomedical Sciences, School of Medicine, Zhejiang University, Hangzhou 310031, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Huang
authors.forename: Xiao-qin
authors.initials: XQ
authors.lastname: Ha
authors.forename: Chu-ze
authors.initials: CZ
authors.lastname: Wu
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000187:drug effects*; D016895:Culture Media, Serum-Free; D017228:Hepatocyte Growth Factor / Q000235:genetics / Q000494:pharmacology*; D009474:Neurons / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D014162:Transfection
chemical_list: D016895:Culture Media, Serum-Free; D017228:Hepatocyte Growth Factor