title: Quantitative analysis of expression of six BmGST genes in silkworm, Bombyx mori.
abstract: Glutathione S-transferases (GSTs) are a multifunctional super gene family, some of which play an important role in insecticide resistance. In this research, we used a real-time quantitative RT-PCR method, and a novel strategy, to measure the transcriptional level per gene copy using an exogenous RNA reference and DNA reference. The transcription levels of six BmGST genes in different tissues of fifth instar Bombyx mori larvae and their responses to insecticide and fluoride were investigated. The results show different levels and patterns of expression of the different BmGSTs in the various tissues observed. The BmGSTs can be induced by insecticide and fluoride, but their responses to each are different. The results of this research are helpful in studying the tissue-specific expression of BmGSTs in Bombyx mori, and in developing new pesticide resistant silkworm varieties.
authors.affiliation: Pre-Clinical Medical and Biological Science College, Soochow University, No. 199 Renai Road, Dushu Lake Higher Education Town, Suzhou Industrial Park, Suzhou 215123, Jiangsu, China.
authors.forename: Guo-Dong
authors.initials: GD
authors.lastname: Zhao
authors.forename: Yi-Ling
authors.initials: YL
authors.lastname: Zhang
authors.forename: Rui-Na
authors.initials: RN
authors.lastname: Gao
authors.forename: Rui-Xian
authors.initials: RX
authors.lastname: Wang
authors.forename: Ting
authors.initials: T
authors.lastname: Zhang
authors.forename: Bing
authors.initials: B
authors.lastname: Li
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Chang-de
authors.initials: CD
authors.lastname: Lu
authors.forename: Wei-de
authors.initials: WD
authors.lastname: Shen
authors.forename: Zheng-Guo
authors.initials: ZG
authors.lastname: Wei
mesh_terms: D000818:Animals; D012831:Bombyx / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics*; D004064:Digestive System / Q000187:drug effects / Q000201:enzymology; D005216:Fat Body / Q000187:drug effects / Q000201:enzymology; D018628:Gene Dosage / Q000235:genetics; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D017344:Genes, Insect / Q000235:genetics*; D005982:Glutathione Transferase / Q000235:genetics* / Q000378:metabolism; D007306:Insecticides / Q000633:toxicity; D007814:Larva / Q000187:drug effects / Q000201:enzymology / Q000235:genetics; D008317:Malpighian Tubules / Q000187:drug effects / Q000201:enzymology; D009928:Organ Specificity / Q000187:drug effects / Q000235:genetics; D012969:Sodium Fluoride / Q000494:pharmacology; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D007306:Insecticides; D012969:Sodium Fluoride; D005982:Glutathione Transferase