title: Preparation and characterization of temperature-responsive poly(N-isopropylacrylamide-co-N,N'-methylenebisacrylamide) monolith for HPLC.
abstract: A temperature-responsive poly(N-isopropylacrylamide-co-N,N'-methylenebisacrylamide) [poly(NIPAAm-co-BIS)] monolith was prepared via a free-radical polymerization technique using an aqueous redox initiator in solution at -12°C. The effect of the % T (total monomer concentration/100 mL) and % C (cross-linker concentration/100 mL) on the visual form was investigated. The effect of the porogen on the pore structure was characterized by SEM. Under the optimum condition, the monolith for HPLC was successfully prepared and its mechanical strength and permeability have been studied. Furthermore, a temperature-dependent resolution of aromatic ketones was achieved using only water as mobile phase. The increasing interaction between solutes and the monolith was observed when temperature increased. The theoretical plate number of every analyte was more than 10(4).
authors.affiliation: College of Pharmacy, Hebei University, Hebei Province Key Laboratory of Pharmaceutical Quality Control, No. 180 Wusi East Road, Baoding 071002, China.
authors.forename: Mingquan
authors.initials: M
authors.lastname: Liu
authors.forename: Haiyan
authors.initials: H
authors.lastname: Liu
authors.forename: Yankun
authors.initials: Y
authors.lastname: Liu
authors.forename: Ligai
authors.initials: L
authors.lastname: Bai
authors.forename: Gengliang
authors.initials: G
authors.lastname: Yang
authors.forename: Chunliu
authors.initials: C
authors.lastname: Yang
authors.forename: Jia
authors.initials: J
authors.lastname: Cheng
mesh_terms: D000178:Acrylamides / Q000138:chemical synthesis / Q000737:chemistry*; D000180:Acrylic Resins; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D057927:Hydrophobic and Hydrophilic Interactions; D007659:Ketones / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D016062:Porosity; D011312:Pressure; D015203:Reproducibility of Results; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature
chemical_list: D000178:Acrylamides; D000180:Acrylic Resins; D007659:Ketones; D011108:Polymers; C052970:poly-N-isopropylacrylamide; C021221:N,N'-methylenebisacrylamide