title: Quantum dot-antisense oligonucleotide conjugates for multifunctional gene transfection, mRNA regulation, and tracking of biological processes.
abstract: It was demonstrated that oligonucleotides, independent of their base sequence and length, could effectively induce the cellular uptake of mercapto acid-capped CdTe QDs after the oligonucleotides were covalently attached on the surface of the QDs. Following these experimental observations, a conjugate composed of covalently linked anti-survivin antisense oligonucleotides (ASON) and CdTe QDs was designed and synthesized. Then, the survivin mRNA down-regulation and the apoptosis of HeLa cells induced by ASON were studied. Systematic experimental results revealed that CdTe-ASON could effectively induce the apoptosis of HeLa cells, while CdTe QDs offered the possibility to visualize the specific intracellular localization of the CdTe-ASON probes strongly associated with their biological functions.
authors.affiliation: Institute of Chemistry, The Chinese Academy of Sciences, Bei Yi Jie 2, Zhong Guan Cun, 100190 Beijing, China.
authors.forename: Yilin
authors.initials: Y
authors.lastname: Li
authors.forename: Xin
authors.initials: X
authors.lastname: Duan
authors.forename: Lihong
authors.initials: L
authors.lastname: Jing
authors.forename: Chunhui
authors.initials: C
authors.lastname: Yang
authors.forename: Ruirui
authors.initials: R
authors.lastname: Qiao
authors.forename: Mingyuan
authors.initials: M
authors.lastname: Gao
mesh_terms: D017209:Apoptosis / Q000235:genetics / Q000502:physiology; D002470:Cell Survival / Q000235:genetics / Q000502:physiology; D006367:HeLa Cells; D006801:Humans; D016376:Oligonucleotides, Antisense / Q000235:genetics*; D016133:Polymerase Chain Reaction; D045663:Quantum Dots; D012333:RNA, Messenger / Q000235:genetics*; D014162:Transfection / Q000379:methods*
chemical_list: D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger