title: [Effect of brazilein on energy metabolism of cerebral ischemia-reperfusion in mice].
abstract: OBJECTIVE
  To investigate brazilein's role in energy metabolism of cerebral ischemia-reperfusion in mice.
  
  
  METHODS
  Fourty mice were randomly divided into the sham group, ischemia group, brazilein 5 mg x kg(-1) group and brazilein 10 mg x kg(-1) group, each with ten cases. Cerebral ischemia model was the built. Mice were injected with brazilein three days before the operation, then they were killed. Cerebrum homogenate was prepared for the detecting of ATP, ADP, AMP and lactic acid by HPLC, expressions of MCT1 and MCT2 in mRNA level by RT-PCR.
  
  
  RESULTS
  The lactic acid in cerebrum increased sharply 20 minutes after cerebral ischemia and decreased 1 hour after reperfusion, then returned to the normal level 24 hours after reperfusion. The charge of energy decreased significantly at the beginning of the ischemia-reperfusion, and the charge restored 1 hour after reperfusion though it was still much lower than the normal level at the time point of 24 hours. Moreover, MCT1 and MCT2 upregulated accompanied with the increase of lactate, MCT2 mRNA enhanced in brazilein 5 mg x kg(-1) group (P < 0.05) while both the two factors increased in brazilein 10 mg x kg(-1) group (P < 0.01).
  
  
  CONCLUSIONS
  Brazilein might protect neurons by changing the charge of energy.
authors.affiliation: Laboratory of Pharmaceutical Sciences, School of Medicine and School of Life Sciences, Beijing 100084, China.
authors.forename: Huiying
authors.initials: H
authors.lastname: Li
authors.forename: Yunyun
authors.initials: Y
authors.lastname: Chen
authors.forename: Fan
authors.initials: F
authors.lastname: Lei
authors.forename: Jun
authors.initials: J
authors.lastname: Hu
authors.forename: Jiaqi
authors.initials: J
authors.lastname: Lan
authors.forename: Yushuang
authors.initials: Y
authors.lastname: Chai
authors.forename: Dongming
authors.initials: D
authors.lastname: Xing
authors.forename: Lijun
authors.initials: L
authors.lastname: Du
mesh_terms: D000818:Animals; D001578:Benzopyrans / Q000008:administration & dosage*; D002545:Brain Ischemia / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism; D004195:Disease Models, Animal; D004734:Energy Metabolism / Q000187:drug effects*; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D007192:Indenes / Q000008:administration & dosage*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D027501:Monocarboxylic Acid Transporters / Q000235:genetics / Q000378:metabolism; D011897:Random Allocation; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism; D027981:Symporters / Q000235:genetics / Q000378:metabolism; D000099233:Monocarboxylate Transport Protein 1
chemical_list: D001578:Benzopyrans; D007192:Indenes; D027501:Monocarboxylic Acid Transporters; D027981:Symporters; D000099233:Monocarboxylate Transport Protein 1; C490426:Slc16a7 protein, mouse; C050295:brazilein