title: Effects of the turn-back primer on intermediate product generation in isothermal DNA amplification.
abstract: In DNA amplification, the initial step of copying a target sequence from the template DNA--the so-called intermediate product generation step--is very important. In examining the turn-back primer (TP)-dependent isothermal DNA amplification (TIA) method, we determined the actual time point of intermediate product generation by extrapolating dsDNA amplification curves. Our results indicate that intermediate product creation is the rate-limiting step in TIA, and good TP design is advantageous for improving the intermediate production process.
authors.affiliation: RIKEN Omics Science Center (OSC), RIKEN Yokohama Institute, Yokohama, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Tanaka
authors.forename: Yasumasa
authors.initials: Y
authors.lastname: Kimura
authors.forename: Yasumasa
authors.initials: Y
authors.lastname: Mitani
authors.forename: Yuki
authors.initials: Y
authors.lastname: Kawai
authors.forename: Alexander
authors.initials: A
authors.lastname: Lezhava
authors.forename: Shohei
authors.initials: S
authors.lastname: Noma
authors.forename: Michihira
authors.initials: M
authors.lastname: Tagami
authors.forename: Jun
authors.initials: J
authors.lastname: Kawai
authors.forename: Yoshihide
authors.initials: Y
authors.lastname: Hayashizaki
authors.forename: Kengo
authors.initials: K
authors.lastname: Usui
mesh_terms: D000465:Algorithms; D017931:DNA Primers / Q000737:chemistry* / Q000235:genetics; D006801:Humans; D008957:Models, Genetic; D008958:Models, Molecular; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D015341:Nucleic Acid Probes / Q000737:chemistry* / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000379:methods
chemical_list: D017931:DNA Primers; D015341:Nucleic Acid Probes