title: Host range of obligate intracellular bacterium  Parachlamydia acanthamoebae.
abstract: The obligate intracellular bacterium Parachlamydia acanthamoebae is a potential human pathogen, but the host range of the bacteria remains unknown. Hence, the growth of P. acanthamoebae Bn₉ in protozoa (Tetrahymena, Acanthamoeba, Dictyostelium) and mammalian cells (HEp-2, Vero, THP-1, PMA-stimulated THP-1, Jurkat) was assessed using an AIU assay which had been previously established by the current authors. P. acanthamoebae grew in Acanthamoeba but not in the other cell types. The growth was also confirmed using DAPI staining, FISH and TEM. These results indicate that the host range of P. acanthamoebae is limited.
authors.affiliation: Department of Medical Laboratory Sciences, Hokkaido University Graduate School of Health Sciences, Sapporo, Hokkaido 060-0812, Japan.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Hayashi
authors.forename: Shinji
authors.initials: S
authors.lastname: Nakamura
authors.forename: Junji
authors.initials: J
authors.lastname: Matsuo
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Fukumoto
authors.forename: Mitsutaka
authors.initials: M
authors.lastname: Yoshida
authors.forename: Kaori
authors.initials: K
authors.lastname: Takahashi
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Mizutani
authors.forename: Takashi
authors.initials: T
authors.lastname: Yao
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Yamaguchi
mesh_terms: D000048:Acanthamoeba / Q000382:microbiology; D056894:Amoebozoa / Q000382:microbiology*; D000818:Animals; D002460:Cell Line; D002695:Chlamydiales / Q000254:growth & development*; D004023:Dictyostelium / Q000382:microbiology; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D002479:Inclusion Bodies / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning