title: Ultrastructural changes in Neurospora cells undergoing senescence induced by kalilo plasmids.
abstract: In N. crassa and N. intermedia, the kalilo plasmid triggers senescence by insertion into mitochondrial DNA. To investigate the cell death pathway induced by this plasmid, juvenile and senescent subcultures of several senescent strains were examined by light and transmission electron microscopy, and at the DNA level. There were no signs of apoptotic events, such as shrinkage of the cytoplasm away from the cell wall, apoptotic bodies, internucleosomal DNA fragmentation or condensation of the cytoplasm while retaining mitochondria and endomembrane structure. Instead, swollen mitochondria lacking cristae and containing amorphous inclusions, and disruption of nuclear and mitochondrial membranes indicated a necrotic mode of cell death.
authors.affiliation: Department of Botany, University of British Columbia, 6270 University Blvd., Vancouver, V6T 1Z4 Canada.
authors.forename: Jin-Woo
authors.initials: JW
authors.lastname: Bok
authors.forename: Ken-Ichiro
authors.initials: K
authors.lastname: Ishida
authors.forename: Anthony J F
authors.initials: AJ
authors.lastname: Griffiths