title: The effect of exercise on the skeletal muscle phospholipidome of rats fed a high-fat diet.
abstract: The aim of this study was to examine the effect of endurance training on skeletal muscle phospholipid molecular species from high-fat fed rats. Twelve female Sprague-Dawley rats were fed a high-fat diet (78.1% energy). The rats were randomly divided into two groups, a sedentary control group and a trained group (125 min of treadmill running at 8 m/min, 4 days/wk for 4 weeks). Forty-eight hours after their last training bout phospholipids were extracted from the red and white vastus lateralis and analyzed by electrospray-ionization mass spectrometry. Exercise training was associated with significant alterations in the relative abundance of a number of phospholipid molecular species. These changes were more prominent in red vastus lateralis than white vastus lateralis. The largest observed change was an increase of ~30% in the abundance of 1-palmitoyl-2-linoleoyl phosphatidylcholine ions in oxidative fibers. Reductions in the relative abundance of a number of phospholipids containing long-chain n-3 polyunsaturated fatty acids were also observed. These data suggest a possible reduction in phospholipid remodeling in the trained animals. This results in a decrease in the phospholipid n-3 to n-6 ratio that may in turn influence endurance capacity.
authors.affiliation: School of Health Sciences, University of Wollongong, NSW 2522, Australia; E-Mail: pelse@uow.edu.au.
authors.forename: Todd W
authors.initials: TW
authors.lastname: Mitchell
authors.forename: Nigel
authors.initials: N
authors.lastname: Turner
authors.forename: Paul L
authors.initials: PL
authors.lastname: Else
authors.forename: Anthony J
authors.initials: AJ
authors.lastname: Hulbert
authors.forename: John A
authors.initials: JA
authors.lastname: Hawley
authors.forename: Jong Sam
authors.initials: JS
authors.lastname: Lee
authors.forename: Clinton R
authors.initials: CR
authors.lastname: Bruce
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Blanksby
mesh_terms: D000818:Animals; D059305:Diet, High-Fat; D005260:Female; D018482:Muscle, Skeletal / Q000378:metabolism* / Q000502:physiology; D010743:Phospholipids / Q000378:metabolism*; D005082:Physical Exertion; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D010743:Phospholipids