title: Cell response to a newly developed Ti-10Ta-10Nb alloy and its sputtered nanoscale coating.
abstract: BACKGROUND
  The success of titanium implants is due to osseointegration or the direct contact of the implant surface and bone without a fibrous connective tissue interface.
  
  
  OBJECTIVE
  The purpose of this study was to evaluate the osteoblast precursor response to titanium - 10 tantalum - 10 niobium (Ti-Ta-Nb) alloy and its sputtered coating.
  
  
  METHODS
  Ti-Ta-Nb coatings were sputtered onto the Ti-Ta-Nb disks. Ti6-Al-4V alloy disks were used as controls. An osteoblast precursor cell line, were used to evaluate the cell responses to the 3 groups. Cell attachment was measured using coulter counter and the cell morphology during attachment period was observed using fluorescent microscopy. Cell culture was performed at 4, 8, 12 and 16 days.
  
  
  RESULTS
  The sputtered Ti-Ta-Nb coatings consisted of dense nanoscale grains in the range of 30 to 100 nm with alpha-Ti crystal structure. The Ti-Ta-Nb disks and its sputtered nanoscale coatings exhibited greater hydrophilicity and rougher surfaces compared to the Ti-6Al-4V disks. The sputtered nanoscale Ti-Ta-Nb coatings exhibited significantly greater cell attachment compared to Ti-6Al-4V and Ti-Ta-Nb disks. Nanoscale Ti-Ta-Nb coatings exhibited significantly greater ALP specific activity and total protein production compared to the other 2 groups.
  
  
  CONCLUSIONS
  It was concluded that nanoscale Ti-Ta-Nb coatings enhance cell adhesion. In addition, Ti-Ta-Nb alloy and its nanoscale coatings enhanced osteoblast differentiation, but did not support osteoblast precursor proliferation compared to Ti-6Al-4V. These results indicate that the new developed Ti-Ta-Nb alloy and its nanoscale Ti-Ta-Nb coatings may be useful as an implant material.
authors.affiliation: Graduate Student, Department of Prosthodontics, Graduate School, Chonnam National University, Gwang-Ju, Korea.
authors.forename: Young-Min
authors.initials: YM
authors.lastname: Kim
authors.forename: Mong-Sook
authors.initials: MS
authors.lastname: Vang
authors.forename: Hong-So
authors.initials: HS
authors.lastname: Yang
authors.forename: Sang-Won
authors.initials: SW
authors.lastname: Park
authors.forename: Hyun-Pil
authors.initials: HP
authors.lastname: Lim