title: A photocleavable rapamycin conjugate for spatiotemporal control of small GTPase activity.
abstract: We developed a novel method to spatiotemporally control the activity of signaling molecules. A newly synthesized photocaged rapamycin derivative induced rapid dimerization of FKBP (FK-506 binding protein) and FRB (FKBP-rapamycin binding protein) upon UV irradiation. With this system and the spatially confined UV irradiation, we achieved subcellularly localized activation of Rac, a member of small GTPases. Our technique offers a powerful approach to studies of dynamic intracellular signaling events.
authors.affiliation: Department of Cell Biology, Center for Cell Dynamics, Johns Hopkins University, Baltimore, Maryland 21205, USA.
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Umeda
authors.forename: Tasuku
authors.initials: T
authors.lastname: Ueno
authors.forename: Christopher
authors.initials: C
authors.lastname: Pohlmeyer
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Nagano
authors.forename: Takanari
authors.initials: T
authors.lastname: Inoue
mesh_terms: D000818:Animals; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008958:Models, Molecular; D041681:NIH 3T3 Cells; D055668:Photochemical Processes; D055503:Protein Multimerization / Q000187:drug effects; D020836:Protein Structure, Quaternary; D020123:Sirolimus / Q000737:chemistry* / Q000494:pharmacology*; D022021:Tacrolimus Binding Proteins / Q000737:chemistry / Q000378:metabolism; D013997:Time Factors; D020744:rac GTP-Binding Proteins / Q000378:metabolism*
chemical_list: D020744:rac GTP-Binding Proteins; D022021:Tacrolimus Binding Proteins; D020123:Sirolimus