title: Serum biochemical characteristics of Beluga, Huso huso (L.), in response to blood sampling after clove powder solution exposure.
abstract: In order to investigate the effect of anesthesia on serum parameters, Beluga, Huso huso (L.) were blood-sampled immediately without anesthesia (control) or subjected to following anesthesia procedure: 40, 120, and 240 s exposure to 3,000, 700, and 500 mg l⁻¹ clove solution, respectively. Blood samples were collected after these periods, when fish were immobile and reached stage 4 anesthesia. Results showed that cortisol and glucose levels were significantly high in 700 and 500 but not 3,000 mg l⁻¹ group compared to control. Serum lactate levels were significantly high in 500 mg l⁻¹ group compared to control group. Lactate levels were not significantly differed between control, 3,000, and 700 mg l⁻¹ groups. There were no significant differences in serum levels of cholesterol, total protein, lactate dehydrogenase, aspartate aminotransferase, alanine aminotransferase, Na⁺, Cl⁻, K⁺, and Ca²⁺. Results suggest that rapid anesthesia with higher dose is better than slow anesthesia with lower dose for blood sampling in Beluga.
authors.affiliation: Faculty of Fisheries and Environment, Department of Fisheries, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran. seyyedmorteza.hoseini@gmail.com
authors.forename: Seyyed Morteza
authors.initials: SM
authors.lastname: Hoseini
authors.forename: Seyed Abbas
authors.initials: SA
authors.lastname: Hosseini
authors.forename: Ali Jafar
authors.initials: AJ
authors.lastname: Nodeh
mesh_terms: D000777:Anesthetics / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D004305:Dose-Response Relationship, Drug; D005399:Fishes / Q000097:blood*; D010936:Plant Extracts / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D027842:Syzygium / Q000737:chemistry*
chemical_list: D000777:Anesthetics; D010936:Plant Extracts