title: [Effects of hypoxic preconditioning on hypoxia tolerance of astrocytes in vitro].
abstract: OBJECTIVE
  To explore the mechanisms of hypoxic preconditioning on protecting cultured astrocytes from hypoxia injury.
  
  
  METHODS
  Cultured astrocytes were divided randomly into several groups: control(C), hypoxia(H) and hypoxic preconditioning (HP). Cells MTT metabolic activity, qualitation of apoptosis and modality to explore the protection effects of hypoxic preconditioning. Immunocytochemistry of Bcl-2 and Bax to explore the mechanisms of hypoxic preconditioning on protecting astrocytes from hypoxia.
  
  
  RESULTS
  Compared with H group there was marked increase of MTT metabolic activity in HP48 and HP72 groups. Immunocytochemistry of Bcl-2 and Bax showed that compared with H group, expression of Bcl-2 was increased in HP group, while expression of Bax was decreased in HP group.
  
  
  CONCLUSIONS
  Hypoxic preconditioning can protect astrocytes from hypoxia. One possible mechanism maybe concerned with inhibition of Bax and maintain of Bcl-2 to depress apoptosis procedure.
authors.affiliation: Department of Physiology, Basic Medical School, Xiangya Hospital, Central South University, Changsha 410078, China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Zhou
authors.forename: Hai-Fu
authors.initials: HF
authors.lastname: Zhang
authors.forename: Fa-Yi
authors.initials: FY
authors.lastname: Liu
authors.forename: Fang
authors.initials: F
authors.lastname: He
authors.forename: Li-Juan
authors.initials: LJ
authors.lastname: Yang
authors.forename: Bai-Sheng
authors.initials: BS
authors.lastname: Huang
authors.forename: Li-Xiang
authors.initials: LX
authors.lastname: Wu
authors.forename: Wei
authors.initials: W
authors.lastname: Wei
mesh_terms: D000222:Adaptation, Physiological / Q000502:physiology*; D000818:Animals; D000831:Animals, Newborn; D017209:Apoptosis / Q000502:physiology*; D001253:Astrocytes / Q000166:cytology / Q000502:physiology*; D015687:Cell Hypoxia; D002478:Cells, Cultured; D019194:Ischemic Preconditioning; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: C494828:Bax protein, rat; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein