title: [Multiglycosides of Tripterygium wilfordii increase sperm apoptosis in male rats].
abstract: OBJECTIVE
  To study the effect of multiglycosides of Tripterygium wilfordii (GTW) on sperm apoptosis in male rats and its possible mechanisms.
  
  
  METHODS
  Sixteen male SD rats were equally assigned to two groups to receive GTW and carboxymethylcellulose (CMC) intragastrically, both at 20 mg/(kg x d) for 6 weeks. Then the epididymal sperm was collected for the measurement of the apoptosis rate, sperm membrane lipid fluidity and the contents of NO, MDA and SOD by flow cytometry and spectrophotometric determination.
  
  
  RESULTS
  After 6 weeks of medication, the GTW group showed a significant increase in sperm apoptosis and contents of NO and MDA (P < 0.01) and a remarkable decrease in sperm membrane lipid fluidity (P < 0.05) and SOD content (P < 0.01) as compared with the CMC control group.
  
  
  CONCLUSIONS
  GTW can damage sperm membrane lipid peroxidation and sperm membrane structure, increase sperm apoptosis, and reduce sperm membrane lipid fluidity.
authors.affiliation: Department of Surgery, Shuangcheng Hospital of Chinese Medicine, Shuangcheng, Heilongfiang 150100, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Zhang
authors.forename: Chun-ying
authors.initials: CY
authors.lastname: Zhang
authors.forename: Bin-Bin
authors.initials: BB
authors.lastname: Li
authors.forename: Hai-feng
authors.initials: HF
authors.lastname: Zhang
authors.forename: Tan
authors.initials: T
authors.lastname: Yuan
authors.forename: Yi-ming
authors.initials: YM
authors.lastname: Fu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002462:Cell Membrane / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006027:Glycosides / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D008560:Membrane Fluidity / Q000187:drug effects; D009569:Nitric Oxide / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D013094:Spermatozoa / Q000187:drug effects*; D013482:Superoxide Dismutase / Q000032:analysis; D029757:Tripterygium / Q000737:chemistry*
chemical_list: D004365:Drugs, Chinese Herbal; D006027:Glycosides; D009569:Nitric Oxide; D008315:Malondialdehyde; D013482:Superoxide Dismutase