title: Identification of new minor metabolites of penicillin G in human serum by multiple-stage tandem mass spectrometry.
abstract: Liquid chromatography/mass spectrometry (LC/MS) and liquid chromatography/tandem mass spectrometry (LC/MS/MS) were applied to characterize drug metabolites. Although these two methods have overcome the identification and structural characterization of metabolites analysis, they remain time-consuming processes. In this study, a novel multiple-stage tandem mass spectrometric method (MS(n) ) was evaluated for identification and characterization of new minor metabolism profiling of penicillin G, one of the β-lactam antibiotics, in human serum. Seven minor metabolites including five phase I metabolites and two phase II metabolites of penicillin G were identified by using data-dependent LC/MS(n) screening in one chromatographic run. The accuracy masses of seven identified metabolites of penicillin G were also confirmed by mass spectral calibration software (MassWorks™). The proposed data-dependent LC/MS(n) method is a powerful tool to provide large amounts of the necessary structural information to characterize minor metabolite in metabolism profiling.
authors.affiliation: Department of Chemistry, National Chung Hsing University, Taichung 40227, Taiwan, ROC.
authors.forename: Hsin-Pin
authors.initials: HP
authors.lastname: Ho
authors.forename: Ren-Jye
authors.initials: RJ
authors.lastname: Lee
authors.forename: Chung-Yu
authors.initials: CY
authors.lastname: Chen
authors.forename: Soo-Ray
authors.initials: SR
authors.lastname: Wang
authors.forename: Zu-Guang
authors.initials: ZG
authors.lastname: Li
authors.forename: Maw-Rong
authors.initials: MR
authors.lastname: Lee
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods; D006801:Humans; D053858:Metabolic Networks and Pathways; D010397:Penicillanic Acid / Q000031:analogs & derivatives / Q000097:blood / Q000737:chemistry / Q000378:metabolism; D010400:Penicillin G / Q000097:blood* / Q000737:chemistry / Q000378:metabolism; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: C008782:penicilloic acid; D010397:Penicillanic Acid; D010400:Penicillin G