title: Lectin purified from Musca domestica pupa up-regulates NO and iNOS production via TLR4/NF-κB signaling pathway in macrophages.
abstract: The present study reported that nitric oxide (NO) was up-regulated by the induction of lectin purified from Musca domestica pupa (MPL) in macrophages without cytotoxicity. The mRNA expression and protein secretion of inducible nitric oxide synthase (iNOS) were strongly induced by MPL treatments. Subsequent investigation revealed that the nuclear factor-κB (NF-κB) inhibitory κB (IκB) in endochylema was inhibited and NF-κB translocated into the nucleus after MPL treatment. Meanwhile, the IKKβ was strongly induced and the production of the toll-like receptor 4 (TLR4) was significantly up-regulated. Moreover, MPL increased NO production via inducing the expression of iNOS through the activation of NF-κB, which suggested that MPL probably acted as an activating agent of the NF-κB activation.
authors.affiliation: Key Laboratory of Food Safety and Sanitation, Ministry of Education, College of food Engineering and Biotechnology, Tianjin University of Science and Technology, No 29, 13th Avenue, Tianjin Economy Technological Development Area, Tianjin 300457, China.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Cao
authors.forename: Minghui
authors.initials: M
authors.lastname: Zhou
authors.forename: Chunling
authors.initials: C
authors.lastname: Wang
authors.forename: Lihua
authors.initials: L
authors.lastname: Hou
authors.forename: Bin
authors.initials: B
authors.lastname: Zeng
mesh_terms: D000818:Animals; D015153:Blotting, Western; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004790:Enzyme Induction; D006793:Houseflies / Q000737:chemistry*; D051550:I-kappa B Kinase / Q000378:metabolism; D037102:Lectins / Q000302:isolation & purification / Q000494:pharmacology*; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D016328:NF-kappa B / Q000378:metabolism*; D009569:Nitric Oxide / Q000096:biosynthesis*; D052247:Nitric Oxide Synthase Type II / Q000096:biosynthesis*; D011679:Pupa / Q000737:chemistry; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D051197:Toll-Like Receptor 4 / Q000378:metabolism*; D051996:Transcription Factor RelA / Q000378:metabolism
chemical_list: D037102:Lectins; D016328:NF-kappa B; C493487:Tlr4 protein, mouse; D051197:Toll-Like Receptor 4; D051996:Transcription Factor RelA; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse; C496564:Chuk protein, mouse; D051550:I-kappa B Kinase