title: Monitoring protein aggregation and toxicity in Alzheimer's disease mouse models using in vivo imaging.
abstract: Aggregation of amyloid beta peptide into senile plaques and hyperphosphorylated tau protein into neurofibrillary tangles in the brain are the pathological hallmarks of Alzheimer's disease. Despite over a century of research into these lesions, the exact relationship between pathology and neurotoxicity has yet to be fully elucidated. In order to study the formation of plaques and tangles and their effects on the brain, we have applied multiphoton in vivo imaging of transgenic mouse models of Alzheimer's disease. This technique allows longitudinal imaging of pathological aggregation of proteins and the subsequent changes in surrounding neuropil neurodegeneration and recovery after therapeutic interventions.
authors.affiliation: Massachusetts General Hospital/Harvard Medical School, 114 16th Street, Charlestown, MA 02129, USA. tspires@partners.org
authors.forename: Tara L
authors.initials: TL
authors.lastname: Spires-Jones
authors.forename: Alix
authors.initials: A
authors.lastname: de Calignon
authors.forename: Melanie
authors.initials: M
authors.lastname: Meyer-Luehmann
authors.forename: Brian J
authors.initials: BJ
authors.lastname: Bacskai
authors.forename: Bradley T
authors.initials: BT
authors.lastname: Hyman
mesh_terms: D000544:Alzheimer Disease / Q000378:metabolism / Q000473:pathology*; D000682:Amyloid / Q000378:metabolism*; D000758:Anesthesia; D000818:Animals; D001921:Brain / Q000378:metabolism / Q000473:pathology; D003399:Craniotomy; D004195:Disease Models, Animal; D005822:Genetic Vectors; D006801:Humans; D007091:Image Processing, Computer-Assisted; D051379:Mice; D036641:Microscopy, Fluorescence, Multiphoton; D016874:Neurofibrillary Tangles / Q000473:pathology; D058225:Plaque, Amyloid / Q000378:metabolism
chemical_list: D000682:Amyloid