title: [The method of long-term potentiation recording in hippocampus in anaesthetized mice in vivo].
abstract: OBJECTIVE
  To establish the method of long-term potentiation (LTP) recording in hippocampus in anaesthetized mice in vivo.
  
  
  METHODS
  Mouse was anaesthetized and then placed in the stereotaxic apparatus. The recording electrode was located at the cell body layer of dentate granule cells and the stimulating electrode at the perforant path according to stereotaxic parameters. Then the LTP was evoked and recorded.
  
  
  RESULTS
  After optimizing of experimental factor, the LTP in PP-DG path in anaesthetized Balb/c mice was successfully recorded. The changes of synaptic plasticity were also observed in SAMP8 and SAMRI by using the optimized method. The results were coincident with the behavioral tests and LTP in hippocampal slices that we reported before.
  
  
  CONCLUSIONS
  The method of LTP recording in vivo in hippocampus in anaesthetized mice was successfully established and could be used to evaluate the synaptic plasticity in vivo.
authors.affiliation: Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Huang
authors.forename: Sheng
authors.initials: S
authors.lastname: Yang
authors.forename: Wen-Xia
authors.initials: WX
authors.lastname: Zhou
authors.forename: Yong-Xiang
authors.initials: YX
authors.lastname: Zhang
mesh_terms: D000758:Anesthesia; D000818:Animals; D004558:Electric Stimulation; D004566:Electrodes; D005071:Evoked Potentials / Q000502:physiology; D006624:Hippocampus / Q000502:physiology*; D017774:Long-Term Potentiation / Q000502:physiology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009473:Neuronal Plasticity / Q000502:physiology*; D013238:Stereotaxic Techniques; D009435:Synaptic Transmission / Q000502:physiology*