title: [Effect of fractalkine on proliferation of pulmonary artery smooth muscle cells].
abstract: OBJECTIVE
  To investigate the effect of fractalkine on cell proliferation of cultured rat pulmonary artery smooth muscle cells (PASMCs) in vitro.
  
  
  METHODS
  Rat PASMCs were cultured in vitro, and treated with different concentrations (10(-10), 10(-9), 10(-8) mol/L) of fractalkine for 12 h, 24 h and 48 h. The cell proliferation was quantified by MTT assay. The cell cycle of PASMCs was measured by flow cytometric(FCM) analysis.
  
  
  RESULTS
  MTT assay showed that fractalkine promoted significantly the proliferation of PASMCs, and the effect was concentration-dependent. FCM analysis indicated that fractalkine increased the percentage of S phase and proliferative index (PI). The percentage of S phase and PI of PASMCs were increased after treated with fractalkine for 12 hours, which reached a maximal level at 24 hours.
  
  
  CONCLUSIONS
  Fractalkine promotes rat PASMCs proliferation in a concentration-dependent manner.
authors.affiliation: Department of Respiratory Medicine, Affiliated Hospital of North Sichuan Medical College, Nanchong 637000, China.
authors.forename: Xiao-Ju
authors.initials: XJ
authors.lastname: Chen
authors.forename: De-Yun
authors.initials: DY
authors.lastname: Cheng
authors.forename: Qiao-Li
authors.initials: QL
authors.lastname: Su
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Fan
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D054428:Chemokine CX3CL1 / Q000494:pharmacology*; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000166:cytology*; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000187:drug effects*; D011651:Pulmonary Artery / Q000166:cytology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D054428:Chemokine CX3CL1