title: [The change of potassium current of neural stem cells cultured in vitro from newborn rat hippocampus].
abstract: OBJECTIVE
  To observe the change of potassium current on cultured neurons differentiated from hippocampus neural stem cells of the newborn rat.
  
  
  METHODS
  Neural stem cells from newborn rat hippocampus were cultured in vitro and passaged continuously. Differentiation of the cell was induced by serum and removing mitogens. After differentiation cells were plated on plastic dishes and cultured for 1 d, 7 d, 14 d and 21 d. Whole-cell voltage patch clamp recording was used respectively to detect voltage-dependent K+ current.
  
  
  RESULTS
  After 1 d culture, no current was detected, and on the 7th d, 14th d, 21st d after differentiation, the amplitude of K+ currents was (18.077 +/- 2.789)pA/pF, (13.099 +/- 2.742)pA/pF, (34.045 +/- 8.067)pA/pF at +50 mV. The recorded K+ current included two components that could be blocked by TEA and 4-AP separately, assumed the slowly inactivating delayed rectifier K+ current (IK) and the fast inactivating transient outward K+ current (IA).
  
  
  CONCLUSIONS
  The function of potassium channels on the hippocampus neural stem cells of the newborn rat approaches mature gradually when the time of differentiation becomes longer in vitro.
authors.affiliation: The Stem Cells Research Center, Medical College of Zhengzhou University, Zhengzhou 450052, China. xingy@zzu.edu
authors.forename: Ying
authors.initials: Y
authors.lastname: Xing
authors.forename: Zi-Juan
authors.initials: ZJ
authors.lastname: Zhang
authors.forename: Ying
authors.initials: Y
authors.lastname: Jing
authors.forename: Xue-Fei
authors.initials: XF
authors.lastname: Han
authors.forename: Yan
authors.initials: Y
authors.lastname: Xu
authors.forename: Wen-Hai
authors.initials: WH
authors.lastname: Yan
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D051668:Delayed Rectifier Potassium Channels / Q000502:physiology; D006624:Hippocampus / Q000166:cytology*; D058953:Neural Stem Cells / Q000166:cytology* / Q000378:metabolism / Q000502:physiology*; D018408:Patch-Clamp Techniques; D015221:Potassium Channels / Q000502:physiology*; D024661:Potassium Channels, Inwardly Rectifying / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D051668:Delayed Rectifier Potassium Channels; D015221:Potassium Channels; D024661:Potassium Channels, Inwardly Rectifying