title: [Effect of reactive oxygen species during the leukemogenic process associated with exposure to benzene].
abstract: OBJECTIVE
  To study the effect of reactive oxygen species (ROS) during the leukemogenic process associated with exposure to benzene.
  
  
  METHODS
  HL-60 was treated with 3 micromol/L benzoquinone (BQ). Generation of ROS in cells was measured by DCFH-DA method. For proliferation assays,cells were stained with alamar blue dye and counted.
  
  
  RESULTS
  ROS production and the proliferation of cell were all increased in BQ-treated cells (13.10 +/- 0.15, 185% +/- 30.00%) as compared with control cells (11.32 +/- 0.09, 100% +/- 0.00%) (P < 0.05); The addition of catalase just before BQ addition reduced ROS generation to basal levels and decreased the growth of cell (P < 0.05).
  
  
  CONCLUSIONS
  ROS may play an important role in the process of proliferation of HL-60 cells induced by BQ.
authors.affiliation: Guangzhou Occupational Disease Prevention & Treatment Centre, Guangzhou, 510620, China.
authors.forename: Yi-min
authors.initials: YM
authors.lastname: Liu
authors.forename: Yong-sheng
authors.initials: YS
authors.lastname: Li
authors.forename: Xu-dong
authors.initials: XD
authors.lastname: Li
authors.forename: Xiao
authors.initials: X
authors.lastname: Guo
mesh_terms: D016227:Benzoquinones / Q000633:toxicity*; D049109:Cell Proliferation / Q000187:drug effects*; D018922:HL-60 Cells / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D006801:Humans; D017382:Reactive Oxygen Species / Q000378:metabolism*
chemical_list: D016227:Benzoquinones; D017382:Reactive Oxygen Species; C004532:quinone