title: [Effect of activation of mitochondrial ATP sensitive potassium channel and calcium activated potassium channel on the permeability transition of mitochondria from both normal and ischemic rat brain].
abstract: OBJECTIVE
  To clarify whether the activation of mitochondrial ATP sensitive potassium channel and calcium activated potassium channel can influence the permeability transition of normal and ischemic brain mitochondria.
  
  
  METHODS
  spectrophotometry was used to determine the effect of the two mitochondrial potassium channel agonists on the swelling of normal and ischemic brain mitochondria respectively.
  
  
  RESULTS
  In normal mitochondria, diazoxide and NS1619 could inhibit the decrease of calcium induced mitochondrial absorbance at 520 nm (A520), which were blocked by atractyloside. When compared with the normal mitochondria, mitochondrial A520 decrease in ischemic brain was even more rapid. Diazoxide and NS1619 could still inhibit the calcium induced mitochondrial A520 decrease, which were blocked by atractyloside.
  
  
  CONCLUSIONS
  Activation of mitochondrial ATP sensitive potassium channel and calcium activated potassium channel can protect brain mitochondria in vitro probably via influencing the mitochondrial permeability transition.
authors.affiliation: Department of Physiology, Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310031, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Shen
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Wu
authors.forename: Wei-Guo
authors.initials: WG
authors.lastname: Liu
authors.forename: Yan
authors.initials: Y
authors.lastname: Lu
authors.forename: Hua-Wei
authors.initials: HW
authors.lastname: Liang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Xia
mesh_terms: D000818:Animals; D001562:Benzimidazoles / Q000494:pharmacology; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D002545:Brain Ischemia / Q000378:metabolism*; D002463:Cell Membrane Permeability; D003981:Diazoxide / Q000494:pharmacology; D054086:KATP Channels / Q000378:metabolism*; D008297:Male; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism; D033681:Mitochondrial Membrane Transport Proteins / Q000187:drug effects*; D000083162:Mitochondrial Permeability Transition Pore; D024681:Potassium Channels, Calcium-Activated / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D001562:Benzimidazoles; D054086:KATP Channels; D033681:Mitochondrial Membrane Transport Proteins; D000083162:Mitochondrial Permeability Transition Pore; D024681:Potassium Channels, Calcium-Activated; C086491:NS 1619; D003981:Diazoxide