title: [Role of HO/CO in IL-beta induced pancreatic islets apoptosis and the effect of fructose-1, 6-disphosphate].
abstract: OBJECTIVE
  To investigate the protective role of HO/CO systems in IL-1beta induced islest apoptosis and to explore the mechanisms of the protective effect of fructose-1, 6-disphosphate (FDP).
  
  
  METHODS
  The pancreases of the rats were removed to collect islets cells. The cells were incubated with IL-1beta with/or FDP. Cell activity, insulin secretion, HO-1 activity, CO content and apoptotic percentage were detected.
  
  
  RESULTS
  HO-1 activity and CO content of the normal control group were low. IL-1beta induced a significant decrease of cell activity and insulin release, flow cytometry analysis showed that apoptotic percentage of islet cells remarkably increased following the addition of IL-1beta, FDP obviously improved the islets cellular activity damaged by IL-1beta, and basic amount of insulin secretion and stimulated by high glucose were improved (P < 0.01). Content of CO and activity of HO-1 were higher in the IL-1beta group than the normal control group (P < 0.05), and there were significant differences between the FDP groups and IL-1beta group. FDP decreased cell apoptotic percentage. Activities of HO-1 and content of CO were higher than that in the IL-1beta group (P < 0.01).
  
  
  CONCLUSIONS
  FDP can attenuate the IL-1beta induced apoptosis of cultured beta cells, the mechanism of which may be improved HO-1 activity and CO content.
authors.affiliation: Department of Phsiology, North Sichuan Medical College, Nanchong 637007, China. zqq7172@hotmail.com
authors.forename: Qian
authors.initials: Q
authors.lastname: Zheng
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
authors.forename: Di-Yong
authors.initials: DY
authors.lastname: Cao
authors.forename: Hai-Tao
authors.initials: HT
authors.lastname: Lan
authors.forename: Hua
authors.initials: H
authors.lastname: Liu
authors.forename: Hua-E
authors.initials: HE
authors.lastname: Jing
authors.forename: Neng
authors.initials: N
authors.lastname: Zhang
authors.forename: Hai-Li
authors.initials: HL
authors.lastname: Fang
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D017209:Apoptosis / Q000187:drug effects*; D002248:Carbon Monoxide / Q000378:metabolism*; D002478:Cells, Cultured; D005260:Female; D005635:Fructosediphosphates / Q000494:pharmacology*; D006419:Heme Oxygenase (Decyclizing) / Q000378:metabolism* / Q000502:physiology; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D053583:Interleukin-1beta / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D007515:Islets of Langerhans / Q000166:cytology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar
chemical_list: D005635:Fructosediphosphates; D007328:Insulin; D053583:Interleukin-1beta; D002248:Carbon Monoxide; D006419:Heme Oxygenase (Decyclizing); C491523:Hmox1 protein, rat; C029063:fructose-1,6-diphosphate