title: XAF1 expression and regulatory effects of somatostatin on XAF1 in prostate cancer cells.
abstract: BACKGROUND
  Somatostatin prevents cell proliferation by inducing apoptosis. Downregulation of the XAF1 transcript may occur during the development of prostate cancer. It is interesting to evaluate the potential regulatory effects of somatostatin on XAF1 expression during the development of prostate cancer cells.
  
  
  METHODS
  XAF1 mRNA and protein expression in human prostate epithelial cells RWPE-1, androgen dependent prostate cancer LNCaP, and androgen independent DU145 and PC3 cells were evaluated using RT-PCR and Western blot. The regulation of XAF1 mRNA and protein expression by somatostatin and its analogue Octreotide was evaluated.
  
  
  RESULTS
  Substantial levels of XAF1 mRNA and proteins were detected in RWPE-1 cells, whereas prostate cancer cells LNCaP, DU145 and PC3 exhibited lower XAF1 expression. Somatostatin and Octreotide up-regulated XAF1 mRNA and protein expression in all prostate cancer cell lines.
  
  
  CONCLUSIONS
  XAF1 down-regulation may contribute to the prostate cancer development. The enhanced XAF1 expression by somatostatin indicates a promising strategy for prostate cancer therapy.
authors.affiliation: Department of Integrated Traditional Chinese and Western Medicine, Qilu Hospital, Shandong University, Jinan, 250012 P.R. China.
authors.forename: Zhaoquan
authors.initials: Z
authors.lastname: Xing
authors.forename: Zunlin
authors.initials: Z
authors.lastname: Zhou
authors.forename: Rong
authors.initials: R
authors.lastname: Yu
authors.forename: Shuling
authors.initials: S
authors.lastname: Li
authors.forename: Chunde
authors.initials: C
authors.lastname: Li
authors.forename: Sten
authors.initials: S
authors.lastname: Nilsson
authors.forename: Zhaoxu
authors.initials: Z
authors.lastname: Liu
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D018931:Antineoplastic Agents, Hormonal / Q000494:pharmacology*; D051017:Apoptosis Regulatory Proteins; D015153:Blotting, Western; D045744:Cell Line, Tumor; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics; D008297:Male; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D015282:Octreotide / Q000494:pharmacology*; D011471:Prostatic Neoplasms / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013004:Somatostatin / Q000494:pharmacology
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D018931:Antineoplastic Agents, Hormonal; D051017:Apoptosis Regulatory Proteins; D047908:Intracellular Signaling Peptides and Proteins; D009363:Neoplasm Proteins; D012333:RNA, Messenger; C417551:XAF1 protein, human; D013004:Somatostatin; D015282:Octreotide