title: Characterization, molecular cloning, and in silico analysis of a novel mannose-binding lectin from Polygonatum odoratum (Mill.) with anti-HSV-II and apoptosis-inducing activities.
abstract: Polygonatum odoratum lectin (POL), a novel mannose-binding lectin with anti-viral and apoptosis-inducing activities, was isolated from rhizomes of Polygonatum odoratum (Mill.) Druce. POL was a homo-tetramer with molecular weight of 11953.623Da per subunits as determined by gel filtration, SDS-PAGE and mass spectrometry. Based on its N-terminal 29-amino acid sequence the full-length cDNA sequence of POL was cloned. Subsequent phylogenetic analysis and molecular modeling revealed that POL belonged to the Galanthus nivalis agglutinin (GNA)-related lectin family, which acquired unique mannose-binding specificity. The hemagglutinating activities of POL were metal ion-independent, and were stable within certain range of pH and temperature alterations. Moreover, POL showed remarkable anti-HSV-II activity towards Vero cells, cytotoxicity towards human melanoma A375 cells and induced apoptosis in a caspase-dependent manner.
authors.affiliation: School of Life Sciences & State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Yang
authors.forename: Huai-long
authors.initials: HL
authors.lastname: Xu
authors.forename: Zi-ting
authors.initials: ZT
authors.lastname: Zhang
authors.forename: Jun-jie
authors.initials: JJ
authors.lastname: Liu
authors.forename: Wen-wen
authors.initials: WW
authors.lastname: Li
authors.forename: Hua
authors.initials: H
authors.lastname: Ming
authors.forename: Jin-ku
authors.initials: JK
authors.lastname: Bao
mesh_terms: D000595:Amino Acid Sequence; D000998:Antiviral Agents / Q000737:chemistry / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D001483:Base Sequence; D003001:Cloning, Molecular; D003603:Cytotoxins / Q000737:chemistry / Q000494:pharmacology; D018258:Herpesvirus 2, Human / Q000187:drug effects*; D006801:Humans; D037241:Mannose-Binding Lectins / Q000737:chemistry* / Q000235:genetics / Q000494:pharmacology; D008545:Melanoma / Q000188:drug therapy*; D008969:Molecular Sequence Data; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology; D037121:Plant Lectins / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology; D031384:Polygonatum / Q000737:chemistry*; D027343:Rhizome / Q000737:chemistry
chemical_list: D000998:Antiviral Agents; D003603:Cytotoxins; D037241:Mannose-Binding Lectins; D010936:Plant Extracts; D037121:Plant Lectins; C063117:snowdrop lectin