title: [Construction and identification of lentiviral vector for RNA interference targeting STUB1 gene].
abstract: To construct and identification of a lentiviral vector for RNA interference (RNAi) targeting STUB1 gene.
  
  
  
  A pair of complementary small hairpin RNA (shRNA) oligonucleotides targeting STUB1 gene was designed, synthesized and inserted into linearized pMagic 4.0 vector. The recombinant plasmid was identified by double restriction digestion with Age I/EcoR I and DNA sequencing.
  
  
  
  PCR and DNA sequencing showed that the shRNA sequence was successfully inserted into pMagic 4.0 vector. The pMagic 4.0 vector was successfully packaged into lentivirus particles.
  
  
  
  A lentiviral shRNA expression vector and particles targeting STUB1 gene has been successfully constructed for the further study of the STUB1 gene.
authors.affiliation: Department of Neurology, The Fourth Affiliated Hospital, Harbin Medical University, Harbin 150001, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhao
authors.forename: Jing-yu
authors.initials: JY
authors.lastname: Zhang
authors.forename: Wan-hai
authors.initials: WH
authors.lastname: Xu
authors.forename: Zi-chao
authors.initials: ZC
authors.lastname: Yang
authors.forename: Qing-jie
authors.initials: QJ
authors.lastname: Zhao
mesh_terms: D018390:Gene Targeting; D005822:Genetic Vectors; D016086:Lentivirus / Q000235:genetics*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D044767:Ubiquitin-Protein Ligases / Q000235:genetics*
chemical_list: D034741:RNA, Small Interfering; C467728:STUB1 protein, human; D044767:Ubiquitin-Protein Ligases