title: Syndecan-1 and syndecan-2 play key roles in herpes simplex virus type-1 infection.
abstract: Herpes simplex virus type 1 (HSV-1) is an important human pathogen and a leading cause of infectious blindness in the developed world. HSV-1 exploits heparan sulfate proteoglycans (HSPG) for attachment to cells. While the significance of heparan sulphate (HS) moieties in HSV-1 infection is well established, the role of specific proteoglycan core proteins in the infection process remains poorly understood. The objective of this study was to assess the roles of syndecan-1 and syndecan-2 core proteins in HSV-1 infection, both of which are expressed by many HSV-1 target cell types. Our results demonstrate that syndecan-1 and syndecan-2 gene silencing by RNA interference reduces HSV-1 entry, plaque formation and facilitates cell survival. Furthermore, HSV-1 infection increases syndecan-1 and syndecan-2 protein synthesis and a resultant increase in cell surface expression of HS. Our observations suggest that changes in syndecan-1 and syndecan-2 expression levels may be related to active viral infection. Taken together, our findings provide new insights into HSPG functions during HSV-1 entry and spread.
authors.affiliation: Department of Ophthalmology and Visual Sciences, University of Illinois at Chicago, College of Medicine, Chicago, IL 60612, USA.
authors.forename: Sarolta
authors.initials: S
authors.lastname: Bacsa
authors.forename: Ghadah
authors.initials: G
authors.lastname: Karasneh
authors.forename: Sandor
authors.initials: S
authors.lastname: Dosa
authors.forename: Jian
authors.initials: J
authors.lastname: Liu
authors.forename: Tibor
authors.initials: T
authors.lastname: Valyi-Nagy
authors.forename: Deepak
authors.initials: D
authors.lastname: Shukla
mesh_terms: D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D020868:Gene Silencing; D018259:Herpesvirus 1, Human / Q000472:pathogenicity*; D054884:Host-Pathogen Interactions; D006801:Humans; D034622:RNA Interference; D053668:Syndecan-1 / Q000037:antagonists & inhibitors / Q000378:metabolism*; D053669:Syndecan-2 / Q000037:antagonists & inhibitors / Q000378:metabolism*; D053586:Virus Internalization
chemical_list: C508241:SDC1 protein, human; C508245:SDC2 protein, human; D053668:Syndecan-1; D053669:Syndecan-2