title: A three-dimensional cell biology model of human hepatocellular carcinoma in vitro.
abstract: We established an in vitro 3-D model of metastatic hepatocellular carcinoma (HCC) by culturing MHCC97H cells on molecular scaffolds within a rotating wall vessel bioreactor. Morphological and biochemical analyses revealed that the 3-D HCC model mirrored many clinical pathological features of HCC in vivo, including cancer cell morphology, tissue ultrastructure, protein production and secretion, glucose metabolism, tissue-specific gene expression, and apoptosis. Xenografts into livers of nude mice resulted in tumorigenesis and distant metastasis. This 3-D HCC spheroid is a promising model for HCC tumor biology, anticancer drug screening, and for the establishment of HCC animal models.
authors.affiliation: Liver Cancer Institute, Zhongshan Hospital, Fudan University and Key Laboratory of Carcinogenesis and Cancer Invasion, Ministry of Education, 136 Xue Yuan Road, Shanghai 200032, People's Republic of China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Tang
authors.forename: Jiefeng
authors.initials: J
authors.lastname: Cui
authors.forename: Rongxin
authors.initials: R
authors.lastname: Chen
authors.forename: Kun
authors.initials: K
authors.lastname: Guo
authors.forename: Xiaonan
authors.initials: X
authors.lastname: Kang
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Dongmei
authors.initials: D
authors.lastname: Gao
authors.forename: Lu
authors.initials: L
authors.lastname: Sun
authors.forename: Changde
authors.initials: C
authors.lastname: Xu
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
authors.forename: Zhaoyou
authors.initials: Z
authors.lastname: Tang
authors.forename: Yinkun
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000418:Albumins / Q000032:analysis; D000818:Animals; D017209:Apoptosis; D006528:Carcinoma, Hepatocellular / Q000378:metabolism / Q000473:pathology*; D002453:Cell Cycle; D045744:Cell Line, Tumor; D020869:Gene Expression Profiling; D005947:Glucose / Q000032:analysis; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D008113:Liver Neoplasms / Q000378:metabolism / Q000473:pathology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D018874:Spheroids, Cellular; D000509:alpha-Fetoproteins / Q000032:analysis; D005723:gamma-Glutamyltransferase / Q000032:analysis
chemical_list: D000418:Albumins; D000509:alpha-Fetoproteins; D007770:L-Lactate Dehydrogenase; D005723:gamma-Glutamyltransferase; D005947:Glucose