title: Glutathione-related antioxidant defense system in elderly patients treated for hypertension.
abstract: The purpose of this study was to analyze glutathione antioxidant defense system in elderly patients treated for hypertension. Studies were carried out in the blood collected from 18 hypertensive and 15 age- and sex-matched controls, all subjects age over 60. Hypertensives were on their usual antihypertensive treatment at the time of blood collection. The concentration of glutathione (GSH) in whole blood and activities of glutathione peroxidase (GPx-1), glutathione transferase (GST), and glutathione reductase (GR) in erythrocytes were measured. The data from patients and controls were compared using independent-samples t test. P value of 0.05 and less was considered statistically significant. We observed increased glutathione-related antioxidant defense in treated hypertensive elderly patients (HT) when compared with healthy controls (C). Mean GSH concentration was significantly higher in HT when compared with C: 3.1 ± 0.29 and 2.6 ± 0.25 mmol/L, respectively, P < 0.001. Mean activity of GR was significantly higher in HT group if compared with C: 83.4 ± 15.25 U/g Hb versus 64.2 ± 8.26 U/g Hb, respectively, P < 0.001. Mean activity of GST was significantly higher in HT group compared with C: 3.0 ± 0.60 mmol CDNB-GSH/mgHb/min and 2.6 ± 0.36 mmol CDNB-GSH/mgHb/min, respectively, P < 0.05. No difference in GPx activity was observed between two groups. These results show that glutathione-related antioxidant defense system was enhanced in elderly hypertensive patients treated for their conditions. This suggests important role of glutathione system in blood pressure regulation. Alterations in concentration and activity of antioxidants observed during antihypertensive medication are likely to be related to the effect of the treatment on NO bioavailability.
authors.affiliation: Department of Biochemistry, Nicolaus Copernicus University in Toruń, L. Rydygier Collegium Medicum in Bydgoszcz, Poland. joanna.rybka1@wp.pl
authors.forename: J
authors.initials: J
authors.lastname: Rybka
authors.forename: D
authors.initials: D
authors.lastname: Kupczyk
authors.forename: K
authors.initials: K
authors.lastname: Kędziora-Kornatowska
authors.forename: J
authors.initials: J
authors.lastname: Motyl
authors.forename: J
authors.initials: J
authors.lastname: Czuczejko
authors.forename: K
authors.initials: K
authors.lastname: Szewczyk-Golec
authors.forename: M
authors.initials: M
authors.lastname: Kozakiewicz
authors.forename: H
authors.initials: H
authors.lastname: Pawluk
authors.forename: L A
authors.initials: LA
authors.lastname: Carvalho
authors.forename: J
authors.initials: J
authors.lastname: Kędziora
mesh_terms: D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D000959:Antihypertensive Agents / Q000627:therapeutic use*; D015415:Biomarkers / Q000097:blood; D001794:Blood Pressure / Q000187:drug effects; D016022:Case-Control Studies; D005260:Female; D005978:Glutathione / Q000097:blood*; D005979:Glutathione Peroxidase / Q000097:blood; D005980:Glutathione Reductase / Q000097:blood; D005982:Glutathione Transferase / Q000097:blood; D006801:Humans; D006973:Hypertension / Q000097:blood / Q000188:drug therapy* / Q000503:physiopathology; D008297:Male; D011044:Poland; D015854:Up-Regulation; D000094765:Glutathione Peroxidase GPX1
chemical_list: D000959:Antihypertensive Agents; D015415:Biomarkers; D005979:Glutathione Peroxidase; D005980:Glutathione Reductase; D005982:Glutathione Transferase; D005978:Glutathione; D000094765:Glutathione Peroxidase GPX1; C000722067:GPX1 protein, human