title: [Effects of endurance exercise on ERK1/2 phosphorylation and expression in skeletal muscle of rats].
abstract: OBJECTIVE
  To investigate the effects of exercise on phosphorylated and total ERK1/2, and mRNA of ERK2.
  
  
  METHODS
  Male rats were randomly divided into control and trained groups. The trained rats were submitted to 1 h or 1.5 h of exercise daily and had a fragment of their excised gastroenemius muscle, 24 h or 48 h after the last training session. The train lasted for 7 weeks. The changes in the expressions of ERK1/2 and p-ERK1/2 were determined by Western blotting.The expression of ERK2 mRNA was determined by RT-PCR.
  
  
  RESULTS
  Exercise led to a marked increase in p-ERK1/2 of trained groups as compared with controls, and increased ERK1/2 protein expression of training 1.5 h/d, 24 h and 48 h after the last training session. ERK2 mRNA was increased by exercise 1 h/d, 24 h and exercise 1.5 h/d, 24 h and 48 h after the last training session. Glucose tolerance test found that blood insulin concentration was decreased with exercise training.
  
  
  CONCLUSIONS
  Endurance exercise could increase muscle responsiveness to insulin by improving the total ERK1/2 and p-ERK1/2, ERK2 mRNA expression.
authors.affiliation: Department of Biochemistry and Molecular Biology, China Medical University, Shenyang 110001, China.
authors.forename: Shi-Cheng
authors.initials: SC
authors.lastname: Cao
authors.forename: Li-Guang
authors.initials: LG
authors.lastname: Sun
authors.forename: Gang
authors.initials: G
authors.lastname: Zhao
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Ye
authors.forename: Huei-Li
authors.initials: HL
authors.lastname: Liu
authors.forename: He
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000818:Animals; D005951:Glucose Tolerance Test; D007328:Insulin / Q000502:physiology; D008297:Male; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000378:metabolism*; D010766:Phosphorylation; D010805:Physical Conditioning, Animal / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D007328:Insulin; D012333:RNA, Messenger; D019950:Mitogen-Activated Protein Kinase 1