title: Transepithelial transport of glucose and mRNA of glucose transporters in the small intestine of rats with iron-deficiency anemia.
abstract: OBJECTIVE
  To evaluate the transepithelial transport of sodium, glucose, potassium, and water and the mRNA level of the sodium-glucose cotransporter (SGLT1) and the facilitated sugar transporter (GLUT2) in the small intestine of iron-deficient rats.
  
  
  METHODS
  After 6 wk of receiving diets with low or normal iron content, rats (Wistar-EPM) were subjected to two experiments: 1) evaluation of the transepithelial transport of sodium, glucose, potassium, and water by an "in vivo" experimental model of intestinal perfusion and 2) determination of relative SGLT1 and GLUT2 mRNA levels in the proximal, intermediate, and distal portions of the small intestine by the northern blotting technique.
  
  
  RESULTS
  Hemoglobin and hepatic iron levels were statistically lower in the anemic rats. The mean transepithelial transports of sodium (-33.0 μEq · min(-1) · cm(-1), glucose (426.0 μM · min(-1) · cm(-1), and water (0.4 μL · min(-1) · cm(-1) in the small intestine of the anemic rats were significantly lower than in the control group (349.1 μEq · min(-1) · cm(-1), 842.6 μM · min(-1) · cm(-1), and 4.3 μl · min(-1) · cm(-1), respectively, P < 0.05). The transepithelial transport of potassium was similar for both groups. The relative SGLT1 mRNA levels of the anemic rats in the intermediate (1.796 ± 0.659 AU) and distal (1.901 ± 0.766 AU) segments were significantly higher than the values for the control rats (intermediate 1.262 ± 0.450 AU, distal 1.244 ± 0.407 AU). No significant difference was observed for the relative SLGT1 mRNA levels in the proximal segment or for the GLUT2 mRNA levels in all segments.
  
  
  CONCLUSIONS
  Iron deficiency decreases the absorption of glucose, sodium, and water and increases SGLT1 mRNA in the intermediate and distal segments of the small intestine of rats.
authors.affiliation: Division of Pediatric Gastroenterology, Federal University of São Paulo, Escola Paulista de Medicina São Paulo, São Paulo, Brazil.
authors.forename: Mônica Lisboa
authors.initials: ML
authors.lastname: Chang Wayhs
authors.affiliation: Division of Pediatric Gastroenterology, Federal University of São Paulo, Escola Paulista de Medicina São Paulo, São Paulo, Brazil. Electronic address: mbmorais@osite.com.br.
authors.forename: Mauro Batista
authors.initials: MB
authors.lastname: de Morais
authors.affiliation: Division of Endocrinology and Metabolism, Department of Physiology and Biophysics, Institute of Biomedical Sciences of the University of São Paulo, São Paulo, Brazil.
authors.forename: Ubiratan Fabres
authors.initials: UF
authors.lastname: Machado
authors.affiliation: Federal University of Santa Catarina, Florianópolis, Santa Catarina, Brazil.
authors.forename: Silvia Modesto
authors.initials: SM
authors.lastname: Nassar
authors.affiliation: Division of Pediatric Gastroenterology, Federal University of São Paulo, Escola Paulista de Medicina São Paulo, São Paulo, Brazil.
authors.forename: Ulysses Fagundes
authors.initials: UF
authors.lastname: Neto
authors.affiliation: Division of Pediatric Gastroenterology, Federal University of São Paulo, Escola Paulista de Medicina São Paulo, São Paulo, Brazil.
authors.forename: Olga Maria
authors.initials: OM
authors.lastname: Silvério Amâncio
mesh_terms: D018798:Anemia, Iron-Deficiency / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001692:Biological Transport; D005947:Glucose / Q000378:metabolism*; D051273:Glucose Transporter Type 2 / Q000235:genetics / Q000378:metabolism*; D006454:Hemoglobins / Q000378:metabolism; D007408:Intestinal Absorption / Q000502:physiology; D007413:Intestinal Mucosa / Q000378:metabolism; D007421:Intestine, Small / Q000378:metabolism*; D019266:Iron, Dietary / Q000008:administration & dosage*; D008099:Liver / Q000378:metabolism; D008297:Male; D011188:Potassium / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012964:Sodium / Q000378:metabolism; D051269:Sodium-Glucose Transporter 1 / Q000235:genetics / Q000378:metabolism*; D014867:Water / Q000378:metabolism
chemical_list: D051273:Glucose Transporter Type 2; D006454:Hemoglobins; D019266:Iron, Dietary; D012333:RNA, Messenger; C495683:Slc2a2 protein, rat; D051269:Sodium-Glucose Transporter 1; D014867:Water; D012964:Sodium; D005947:Glucose; D011188:Potassium