title: Cell viability effects and antioxidant and antimicrobial activities of Tunisian date syrup (Rub El Tamer) polyphenolic extracts.
abstract: The aqueous-acetone polyphenolic extract of the traditionally derived date syrup, known as "Rub El Tamer", was analyzed using RP-HPLC-DAD and ESI-MS. The phenolic content of the extract was 394.53 ± 1.13 mg per 100 g of syrup with caffeoylsinapylquinic acid as the most abundant compound (72.23%). The extract exhibited strong antioxidant activities as evaluated using the ABTS (2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)), DPPH (2,2-diphenyl-1-picrylhydrazyl) and FRAP (ferric reducing antioxidant power) methods. The extract antimicrobial potential against a range of microorganism strains showed that Staphylococcus aureus, Staphylococcus epidermidis, and Bacillus cereus were the most sensitive bacteria with MBC in the range of 0.5-0.05 mg/mL. Furthermore, in the presence of the syrup extract (8.18-131 μg/mL), the Human SH-SY5Y neuroblastoma and the 3T3 fibroblast cell lines showed dissimilar reduction of viability suggesting a higher cytotoxic effect against tumorigenic cells. Our results provide new insights into date syrup characterization which should stimulate further studies of this hot desert resource.
authors.affiliation: Food Biochemistry, INSAT, University of 7 November at Carthage, Tunis, Tunisia.
authors.forename: Karima
authors.initials: K
authors.lastname: Dhaouadi
authors.forename: Faten
authors.initials: F
authors.lastname: Raboudi
authors.forename: Carmen
authors.initials: C
authors.lastname: Estevan
authors.forename: Enrique
authors.initials: E
authors.lastname: Barrajón
authors.forename: Eugenio
authors.initials: E
authors.lastname: Vilanova
authors.forename: Mohamed
authors.initials: M
authors.lastname: Hamdaoui
authors.forename: Sami
authors.initials: S
authors.lastname: Fattouch
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis / Q000494:pharmacology*; D000975:Antioxidants / Q000032:analysis* / Q000494:pharmacology; D028023:Arecaceae / Q000737:chemistry*; D001419:Bacteria / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects*; D005419:Flavonoids / Q000032:analysis / Q000494:pharmacology*; D005638:Fruit / Q000737:chemistry; D006801:Humans; D051379:Mice; D041681:NIH 3T3 Cells; D010636:Phenols / Q000032:analysis / Q000494:pharmacology*; D010936:Plant Extracts / Q000032:analysis / Q000494:pharmacology*; D059808:Polyphenols; D014416:Tunisia
chemical_list: D000900:Anti-Bacterial Agents; D000975:Antioxidants; D005419:Flavonoids; D010636:Phenols; D010936:Plant Extracts; D059808:Polyphenols