title: Actinomycetospora rishiriensis sp. nov., isolated from a lichen.
abstract: An actinomycete, strain RI109-Li102(T), was isolated from a lichen sample obtained from Rishiri Island in Japan. Cells of strain RI109-Li102(T) were Gram-positive, aerobic and non-motile and formed bud-like spore chains. The isolate grew with 0-3 % (w/v) NaCl, at pH 5-9 and at 10-30 °C (optimum 30 °C). The whole-cell hydrolysate contained meso-diaminopimelic acid, arabinose and galactose. The predominant menaquinone was MK-8(H(4)) and the diagnostic phospholipids were phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol and diphosphatidylglycerol. The major cellular fatty acids were iso-C(16 : 0) and iso-C(16 : 1) H. Comparative 16S rRNA gene sequence analysis revealed that strain RI109-Li102(T) was most closely related to Actinomycetospora corticicola 014-5(T) (99.0 % rRNA gene sequence similarity) and Actinomycetospora chiangmaiensis YIM 0006(T) (98.4 %). However, DNA-DNA hybridization assays, as well as physiological and biochemical analyses, showed that strain RI109-Li102(T) could be differentiated from its closest phylogenetic relatives. It is proposed that strain RI109-Li102(T) ( = NBRC 106356(T) = KCTC 19782(T)) be classified as the type strain of a novel species, with the name Actinomycetospora rishiriensis sp. nov.
authors.affiliation: Division of Applied Biological Sciences, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Takeda-4, Kofu 400-8511, Japan.
authors.forename: Hideki
authors.initials: H
authors.lastname: Yamamura
authors.affiliation: Division of Applied Biological Sciences, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Takeda-4, Kofu 400-8511, Japan.
authors.forename: Haruna
authors.initials: H
authors.lastname: Ashizawa
authors.affiliation: Division of Applied Biological Sciences, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Takeda-4, Kofu 400-8511, Japan.
authors.forename: Youji
authors.initials: Y
authors.lastname: Nakagawa
authors.affiliation: NITE Biological Resource Center, National Institute of Technology and Evaluation, Kazusakamatari 2-5-8, Kisarazu, Chiba 292-0818, Japan.
authors.forename: Moriyuki
authors.initials: M
authors.lastname: Hamada
authors.affiliation: NITE Biological Resource Center, National Institute of Technology and Evaluation, Kazusakamatari 2-5-8, Kisarazu, Chiba 292-0818, Japan.
authors.forename: Yuumi
authors.initials: Y
authors.lastname: Ishida
authors.affiliation: NITE Biological Resource Center, National Institute of Technology and Evaluation, Kazusakamatari 2-5-8, Kisarazu, Chiba 292-0818, Japan.
authors.forename: Misa
authors.initials: M
authors.lastname: Otoguro
authors.affiliation: NITE Biological Resource Center, National Institute of Technology and Evaluation, Kazusakamatari 2-5-8, Kisarazu, Chiba 292-0818, Japan.
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Tamura
authors.affiliation: Division of Applied Biological Sciences, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Takeda-4, Kofu 400-8511, Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Hayakawa
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000378:metabolism; D007564:Japan; D008011:Lichens / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012965:Sodium Chloride / Q000378:metabolism
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride