title: [Changes of HO-1 genes expression in liver following ischemia/reperfusion of limbs and their significance in rats].
abstract: OBJECTIVE
  To detect the changes of inducible heme oxygenase (HO-1) expression in liver following ischemia/reperfusion (I/R) of hindlimbs and to elucidate their significance.
  
  
  METHODS
  I/R was established using the occlusion of the femoral arteries for 4h and reopening for 2-24 h in rats. The expression of HO-1 mRNA and HO-1 protein in liver tissue were detected with reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemical technique, respectively. The observation of pathologic changes of liver was made following the inhibition of HO-1 by zinc protoporphyrin (ZnPP).
  
  
  RESULTS
  Compared with control groups, the relative expression level of HO-1 mRNA significantly increased in I/R group. There were more HO-1 positive hepatocytes in I/R group than control groups. The pathologic changes of liver tissue became more severe in I/R + ZnPP group.
  
  
  CONCLUSIONS
  The expressions of HO-1 mRNA and protein in liver tissue are significantly upregulated, induction of HO-1 is involved in protection for hepatocytes during the I/R of hindlimbs.
authors.affiliation: Department of Anatomy, Hebei Medical University, Shijiazhuang 050017, China.
authors.forename: Zhong-Li
authors.initials: ZL
authors.lastname: Shi
authors.forename: Yi-Ling
authors.initials: YL
authors.lastname: Ling
authors.forename: Yi-Qun
authors.initials: YQ
authors.lastname: Ling
authors.forename: Yu-Xia
authors.initials: YX
authors.lastname: Yao
authors.forename: Jun-Lin
authors.initials: JL
authors.lastname: Zhou
mesh_terms: D000818:Animals; D015870:Gene Expression; D006419:Heme Oxygenase (Decyclizing) / Q000235:genetics / Q000378:metabolism*; D006614:Hindlimb / Q000098:blood supply; D008099:Liver / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D006419:Heme Oxygenase (Decyclizing); C491523:Hmox1 protein, rat