title: The effect of glycogen phosphorolysis on basal glutaminergic transmission.
abstract: Astrocytic glycogen metabolism sustains neuronal activity but its impact on basal glutamatergic synaptic transmission is not clear. To address this issue, we have compared the effect of glycogen breakdown inhibition on miniature excitatory postsynaptic currents (mEPSCs) in rat hippocampal pure neuronal culture (PNC) and in astrocyte-neuronal co-cultures (ANCC). Amplitudes of mEPSC in ANCC were nearly twice as large as in PNC with no difference in current kinetics. Inhibition of glycogen phosphorylase reduced mEPSC amplitude by roughly 40% in ANCC being ineffective in PNC. Altogether, these data indicate that astrocyte-neuronal interaction enhances basal mEPSCs in ANCC mainly due to astrocytic glycogen metabolism.
authors.affiliation: Department of Animal Molecular Physiology and Laboratory of Cellular Neurobiology, Institute of Zoology, Wrocław University, Cybulskiego 30, 50-205 Wrocław, Poland.
authors.forename: Jerzy
authors.initials: J
authors.lastname: Mozrzymas
authors.forename: Tomasz
authors.initials: T
authors.lastname: Szczęsny
authors.forename: Darek
authors.initials: D
authors.lastname: Rakus
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000378:metabolism / Q000502:physiology; D018920:Coculture Techniques; D018698:Glutamic Acid / Q000378:metabolism*; D006003:Glycogen / Q000378:metabolism*; D024981:Glycogen Phosphorylase / Q000037:antagonists & inhibitors / Q000378:metabolism; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D009474:Neurons / Q000378:metabolism / Q000502:physiology; D011805:Quinolinic Acids / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D009435:Synaptic Transmission
chemical_list: C110474:Bay W1807; D011805:Quinolinic Acids; D018698:Glutamic Acid; D006003:Glycogen; D024981:Glycogen Phosphorylase