title: Changes in expression of skeletal muscle proteins between obesity-prone and obesity-resistant rats induced by a high-fat diet.
abstract: A primary goal in obesity research is to determine why some people become obese (obesity-prone, OP) and others do not (obesity-resistant, OR) when exposed to high-calorie diets. The metabolic changes that cause reduced adiposity and resistance to obesity development have yet to be determined. We thus performed proteomic analysis on muscular proteins from OP and OR rats in order to determine whether other novel molecules are involved in this response. To this end, rats were fed a low- or high-fat diet for 8 weeks and were then classified into OP and OR rats by body weight gain. OP rats gained about 25% more body weight than OR rats, even though food intake did not differ significantly between the two groups. Proteomic analysis using 2-DE demonstrated differential expression of 26 spots from a total of 658 matched spots, of which 23 spots were identified as skeletal muscle proteins altered between OP and OR rats by peptide mass fingerprinting. Muscle proteome data enabled us to draw the conclusion that enhanced regulation of proteins involved in lipid metabolism and muscle contraction, as well as increased expression of marker proteins for oxidative muscle type (type I), contributed to obesity-resistance; however, antioxidative proteins did not.
authors.affiliation: Department of Biotechnology, Daegu University, Kynungsan, Kyungbuk, Republic of Korea.
authors.forename: Dong Hyun
authors.initials: DH
authors.lastname: Kim
authors.forename: Jung-Won
authors.initials: JW
authors.lastname: Choi
authors.forename: Jeong In
authors.initials: JI
authors.lastname: Joo
authors.forename: Xia
authors.initials: X
authors.lastname: Wang
authors.forename: Duk Kwon
authors.initials: DK
authors.lastname: Choi
authors.forename: Tae Seok
authors.initials: TS
authors.lastname: Oh
authors.forename: Jong Won
authors.initials: JW
authors.lastname: Yun
mesh_terms: D000818:Animals; D001835:Body Weight / Q000502:physiology; D004032:Diet; D004041:Dietary Fats / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D008297:Male; D009124:Muscle Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000502:physiology*; D009765:Obesity / Q000503:physiopathology*; D020543:Proteome / Q000032:analysis; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D015430:Weight Gain / Q000502:physiology
chemical_list: D004041:Dietary Fats; D009124:Muscle Proteins; D020543:Proteome