title: Generation of neutrophil priming activity by cell-containing blood components treated with pathogen reduction technology and stored in platelet additive solutions.
abstract: BACKGROUND
  Storage of cell-containing blood components such as platelet concentrates (PCs) and red blood cells (RBCs) results in generation of biologically active compounds, many of which may be associated with adverse transfusion events. Priming of the neutrophil oxidase activity is a common characteristic of many of the biologically active compounds found in stored blood. We evaluated the priming activity of pathogen reduction technology (PRT)-treated PCs stored in plasma or platelet additive solution (PAS) and PRT-treated RBCs.
  
  
  METHODS
  PCs were collected with Trima or Amicus equipment and were PRT treated with the Mirasol PRT system or the Intercept Blood System. Some units were gamma irradiated. Products were stored in 100% plasma or 35% plasma plus PAS. RBCs were washed and PRT treated before storage. Samples were removed throughout storage and priming of the oxidase activity was measured.
  
  
  RESULTS
  Platelets collected on Trima or Amicus equipment and stored in plasma or PAS demonstrated increasing priming activity during 5 to 7 days of storage. Gamma irradiation, but not PRT treatment with either technology, further enhanced this priming activity. Supernatants of RBCs stored for 42 days induced priming in untreated controls, but not in washed or Mirasol PRT-treated test products.
  
  
  CONCLUSIONS
  Production of oxidase priming activity increased during storage in all blood products. No significant differences were associated with the collection method, storage in PAS, or PRT treatment. The generation of biologically active compounds, which may serve as an etiology for adverse events, appears to be independent of these processes for collection, storage, and pathogen reduction.
authors.affiliation: Bonfils Blood Center and the Center for Cancer and Blood Disorders, The Children's Hospital, Denver, Colorado, USA.
authors.forename: Daniel R
authors.initials: DR
authors.lastname: Ambruso
authors.forename: Gail
authors.initials: G
authors.lastname: Thurman
authors.forename: Khoa
authors.initials: K
authors.lastname: Tran
authors.forename: Susanne
authors.initials: S
authors.lastname: Marschner
authors.forename: Birgit
authors.initials: B
authors.lastname: Gathof
authors.forename: Karin
authors.initials: K
authors.lastname: Janetzko
authors.forename: Raymond P
authors.initials: RP
authors.lastname: Goodrich
mesh_terms: D001792:Blood Platelets / Q000378:metabolism / Q000528:radiation effects; D001793:Blood Preservation / Q000379:methods*; D006801:Humans; D009504:Neutrophils / Q000166:cytology / Q000378:metabolism*