title: Fabrication of polymeric ionic liquid/graphene nanocomposite for glucose oxidase immobilization and direct electrochemistry.
abstract: A novel polymeric ionic liquid functionalized graphene, poly(1-vinyl-3-butylimidazolium bromide)-graphene (denoted as poly(ViBuIm(+)Br(-))-G), was synthesized. FTIR, UV-vis spectra and TEM were used to characterize the formation of as synthesized nanocomposites. Due to the modification of the polymeric ionic liquid, poly(ViBuIm(+)Br(-))-G can not only be dispersed well in aqueous solutions to form a homogeneous colloidal suspension of individual nanosheets, but also exhibit a strong positive charge. Based on self-assembly, the negatively charged glucose oxidase (GOD) was immobilized onto the poly(ViBuIm(+)Br(-))-G to form a GOD/poly(ViBuIm(+)Br(-))-G/glassy carbon (GC) electrode under mild conditions. With the advantage of both poly(ViBuIm(+)Br(-)) and graphene, poly(ViBuIm(+)Br(-))-G can provide a favorable and conductive microenvironment for the immobilized GOD and thus promote their direct electron transfer at the GC electrode. Furthermore, the GOD/poly(ViBuIm(+)Br(-))-G/GC electrode displayed an excellent sensitivity, together with a wide linear range and excellent stability for the detection of glucose. Accordingly, these unique properties of such novel nanocomposite generate a promising platform for the construction of mediator-free enzymatic biosensors.
authors.affiliation: Liaoning Provincial Key Laboratory for Green Synthesis and Preparative Chemistry of Advanced Materials (Liaoning University), Shenyang 110036, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.forename: Shuyao
authors.initials: S
authors.lastname: Wu
authors.forename: Ling
authors.initials: L
authors.lastname: Zhang
authors.forename: Jin
authors.initials: J
authors.lastname: Lu
authors.forename: Francis
authors.initials: F
authors.lastname: Verproot
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Xing
authors.forename: Jinghong
authors.initials: J
authors.lastname: Li
authors.forename: Xi-Ming
authors.initials: XM
authors.lastname: Song
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D003217:Conductometry / Q000295:instrumentation*; D004800:Enzymes, Immobilized / Q000737:chemistry; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005947:Glucose / Q000032:analysis* / Q000737:chemistry; D005949:Glucose Oxidase / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry*; D052578:Ionic Liquids / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry*; D036103:Nanotechnology / Q000295:instrumentation; D011108:Polymers / Q000737:chemistry
chemical_list: D004800:Enzymes, Immobilized; D052578:Ionic Liquids; D011108:Polymers; D006108:Graphite; D005949:Glucose Oxidase; D005947:Glucose