title: Cloning and characterization of a β-1,3-glucan-binding protein from shrimp Fenneropenaeus chinensis.
abstract: The β-1,3-D: -glucan binding protein (βGBP), one kind of the pattern recognition proteins (PRPs), was cloned and characterized from the Chinese Shrimp Fenneropenaeus chinensis, and named as FcβGBP-HDL. The results indicated that the full length cDNA of 6713 bp had an open reading frame encoded a polypeptide of 2139 amino acids with two glucanase-like motifs and one RGD motif, while without signal peptide. The calculated molecular mass of mature protein was 240.7 kDa and theoretical pI was 5.95. Sequence comparison of the deduced amino acid sequence of FcβGBP-HDL showed varied identity of 88, 54 and 53% with those of Litopenaeus vannamei βGBP-HDL, Pacifastacus leniusculus βGBP, and Pontastacus leptodactylus βGBP, respectively. qRT-PCR analysis indicated that FcβGBP-HDL was expressed in intestines, hepatopancreas, muscle, gill and hemocytes, and its profile was modified post WSSV challenge. The differential expressions of FcβGBP-HDL in different tissues post WSSV challenge suggested that FcβGBP-HDL might play an important role in shrimp immune and perform differently in different tissues. These data would be helpful to better understand the WSSV-resistance mechanism of farming shrimp.
authors.affiliation: College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China.
authors.forename: Xiaofang
authors.initials: X
authors.lastname: Lai
authors.forename: Jie
authors.initials: J
authors.lastname: Kong
authors.forename: Qingyin
authors.initials: Q
authors.lastname: Wang
authors.forename: Weiji
authors.initials: W
authors.lastname: Wang
authors.forename: Xianhong
authors.initials: X
authors.lastname: Meng
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D037102:Lectins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D033561:Penaeidae / Q000235:genetics* / Q000821:virology; D010802:Phylogeny; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D046848:White spot syndrome virus 1 / Q000502:physiology; D047071:beta-Glucans / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D018076:DNA, Complementary; D037102:Lectins; D047071:beta-Glucans; C037937:glucan-binding proteins; C033363:beta-1,3-glucan