title: [Differences of wound contraction and apoptosis in full-thickness burn wounds repaired with different artificial dermal stent in pigs].
abstract: OBJECTIVE
  To investigate the roles and differences of angiogenesis of different dermal scaffolds on wound contraction and apoptosis during full-thickness burn wound repair.
  
  
  METHODS
  Wounds were observed at different time after the collagen-sulfonated carboxymethyl chitosan porous scaffold or collagen-chitosan porous scaffold or acellular dermal matrix were respectively transplanted on wounds of full thickness burn with debridement in Bama miniature pigs. At the same time, vessels and myo-fibroblasts expressing α-smooth muscle action(α-SMA) and apoptosis in wounds of different time were detected in situ by immunohistochemical staining, terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate-biotin nick end labeling. The burn wounds without any scaffold transplantation were studied as the control.
  
  
  RESULTS
  Wounds with different scaffolds transplantation were different from granulation wounds. Vessels expressing α-SMA had been increasing continuously in the wounds from 1 to 3 weeks after different scaffolds transplantation and decreased in wounds after epidermis had been grafted for 2 weeks on surface of the scaffolds transplanted on wounds for 2 weeks. Vessels expressing α-SMA were the most in the wounds with collagen-sulfonated carboxymethyl chitosan porous scaffold transplantation and the least in the control wounds without dermal scaffold at different time. Myo-fibroblasts expressing α-SMA was the least in the wounds with collagen-sulfonated carboxymethyl chitosan porous scaffold transplantation and the peak of expressions was on the 2nd week, however, the peak in the wounds with the other two scaffolds transplantation and in the control wound without dermal scaffold was on the 3rd week. Myo-fibroblasts expressing α-SMA was the most in the control wounds. Apoptosis had been increasing continuously in the transplantation wounds from 2 to 4 weeks after different scaffolds transplantation, however, apoptosis had begun to increase continuously from 3 to 4 weeks in the control wounds. Apoptosis was the most in the wounds with collagen-sulfonated carboxymethyl chitosan porous scaffold transplantation and the least in the control wounds without dermal scaffold from 3 to 4 weeks.
  
  
  CONCLUSIONS
  Sulfonated carboxymethyl chitosan can promote migration of reparative cells and angiogenesis, and it can repair full-thickness burn wound fast and well.
authors.affiliation: Department of Plastic Surgery, Zhejiang University of Chinese Medicine, Hangzhou 310006, China. shaojunxu5@yahoo.com.cn
authors.forename: Shao-jun
authors.initials: SJ
authors.lastname: Xu
authors.forename: Lie
authors.initials: L
authors.lastname: Ma
authors.forename: Jian-ying
authors.initials: JY
authors.lastname: Teng
authors.forename: Jing
authors.initials: J
authors.lastname: Xie
authors.forename: Jin-tu
authors.initials: JT
authors.lastname: Zhu
authors.forename: Dong-jie
authors.initials: DJ
authors.lastname: Sun
authors.forename: Yong-guang
authors.initials: YG
authors.lastname: Wang
authors.forename: You-di
authors.initials: YD
authors.lastname: Ni
authors.forename: Ting
authors.initials: T
authors.lastname: Lou
mesh_terms: D000818:Animals; D017209:Apoptosis; D002056:Burns / Q000473:pathology / Q000601:surgery*; D048271:Chitosan / Q000031:analogs & derivatives* / Q000494:pharmacology; D003094:Collagen; D004195:Disease Models, Animal; D005260:Female; D016038:Skin Transplantation; D017768:Skin, Artificial; D013552:Swine; D054457:Tissue Scaffolds
chemical_list: C514968:carboxymethyl-chitosan; D003094:Collagen; D048271:Chitosan