title: [Mechanism of protective effect of pretreatment of paeoniflorin on injured cortical neurons with corticosterone].
abstract: OBJECTIVE
  Using the method of reverse transcription-polymerase chain reaction (RT-PCR) to detect the related gene expression,with a view to clarify the principal mechanism that the neuroprotection of paeoniflorin (PF).
  
  
  METHODS
  At the seventh day, after the pretreatment of PF (0.5, 2, 10 micromol x L(-1)), with 30 min primary cultures of neurons from the cerebral cortex of 16 days old embryo rats were injured with corticosterone (200 micromol x L(-1)). After 48 h, the methods of RT-PCR was used to detect the expression of Bcl-2, Bax, Caspase-3, BDNF mRNA.
  
  
  RESULTS
  Compared with model group, the dose of PF(2, 10 micromol x L(-1)) not only can degrade the expression of Bax, Caspase-3 mRNA, but also can raise the expression of Bcl-2, BDNF mRNA notablely (P < 0.05).
  
  
  CONCLUSIONS
  The data indicated that the principal mechanism of prophylactic neuroprotective effect of PF on corticosterone-induced neurons damages maybe due to PF can raise the expression of apoptosis-related genes Bel-2, Bax, Caspase-3 mRNA, and also ascribe to PF can raise the expression of neurotrophic factor.
authors.affiliation: Tianjin University of Traditional Chinese Medicine, Tianjin Laboratory of Chinese Medience Pharrnachology, Tianjin 300193, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Guangzhi
authors.initials: G
authors.lastname: Cui
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xiumei
authors.initials: X
authors.lastname: Gao
authors.forename: Yan
authors.initials: Y
authors.lastname: Gao
mesh_terms: D000818:Animals; D001565:Benzoates / Q000494:pharmacology*; D019208:Brain-Derived Neurotrophic Factor / Q000235:genetics / Q000378:metabolism; D001952:Bridged-Ring Compounds / Q000494:pharmacology*; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology* / Q000187:drug effects / Q000293:injuries / Q000378:metabolism; D003345:Corticosterone / Q000009:adverse effects; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D005960:Glucosides / Q000494:pharmacology*; D008297:Male; D039821:Monoterpenes; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D018696:Neuroprotective Agents / Q000494:pharmacology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D006027:Glycosides
chemical_list: D001565:Benzoates; D019208:Brain-Derived Neurotrophic Factor; D001952:Bridged-Ring Compounds; D053148:Caspase 3; D003345:Corticosterone; D005960:Glucosides; D039821:Monoterpenes; D018696:Neuroprotective Agents; D019253:Proto-Oncogene Proteins c-bcl-2; C015423:peoniflorin; D006027:Glycosides