title: Resazurin dye as a reliable tool for determination of cell number and viability in mesenchymal stem cell culture.
abstract: Human mesenchymal stem cells are a valuable cell source for tissue engineering. Determination of cell number and viability is crucial. However, this can be tested only at the end of cell culture. This study shows that Resazurin dye staining is a reliable tool for evaluation of cell number and viability in culture without cell perturbation.
authors.affiliation: Department for Traumatology, University Hospital Regensburg, Germany. Thomas.Dienstknecht@klinik.uniregensburg.de
authors.forename: T
authors.initials: T
authors.lastname: Dienstknecht
authors.forename: K
authors.initials: K
authors.lastname: Ehehalt
authors.forename: Zsuzsa
authors.initials: Z
authors.lastname: Jenei-Lanzl
authors.forename: J
authors.initials: J
authors.lastname: Zellner
authors.forename: Michael
authors.initials: M
authors.lastname: Müller
authors.forename: A
authors.initials: A
authors.lastname: Berner
authors.forename: M
authors.initials: M
authors.lastname: Nerlich
authors.forename: P
authors.initials: P
authors.lastname: Angele
mesh_terms: D002452:Cell Count / Q000379:methods*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects; D010078:Oxazines / Q000009:adverse effects*; D023822:Tissue Engineering; D014966:Xanthenes / Q000009:adverse effects*
chemical_list: D010078:Oxazines; D014966:Xanthenes; C005843:resazurin