title: [Effect of nitric oxide synthase inhibitors on the changes of hemodynamic parameters and aortic tension induced by septic shock in rats].
abstract: OBJECTIVE
  To investigate the effect of three types of nitric oxide synthase inhibitors on the changes of hemodynamic parameters and thoracic aorta tension induced by septic shock in rats.
  
  
  METHODS
  We used cecal ligation and puncture (CLP) method to establish septic shock in rats, and the three types of nitric oxide synthase inhibitors were injected after CLP. The carotid artery was cannulated and connected to a pressure transducer to determine mean arterial blood pressure (MABP). Ventricular dynamic parameters were determined following intraventricular cannulation via the carotid artery, including heart rate (HR), left ventricular developed pressure (LVDP), maximal rise/fall velocity of ventricular pressure (+/- dP/dt(max)). Isolated thoracic rings were mounted on an organ bath and the tension of the vessel was recorded.
  
  
  RESULTS
  (1) After using L-NAME, AMG and 7-NI the mortality decreased to 50.0%, 37.5%, and 42.1%, respectively (from 65.2% in septic shock rats); (2) The MABP in septic shock rats partly recovered after using the NOS inhibitors, all ventricular dynamic parameters partly recovered after using the inhibitors; (3) The hyporeactivity of endothelium-denuded aortic rings to vasoconstrictors induced by septic shock was partly recovered by pretreatment with the inhibitors. However, only L-NAME or 7-NI could inhibit the decrease of vasoconstriction induced by septic shock in endothelium-intact aortic rings.
  
  
  CONCLUSIONS
  The three types of nitric oxide synthase inhibitors can improve the hemodynamic parameters and vasoconstriction responsiveness of endothelium-denuded aorta of septic shock rats. Furthermore, L-NAME and 7-NI improve the responsiveness of endothelium-intact aorta.
authors.affiliation: Department of Physiology, Jiaxing College School of Medicine, Jiaxing 314001, China.
authors.forename: Man-Li
authors.initials: ML
authors.lastname: Xia
authors.forename: Xin-Mei
authors.initials: XM
authors.lastname: Zhou
authors.forename: Ce
authors.initials: C
authors.lastname: Xu
authors.forename: Ling-Bo
authors.initials: LB
authors.lastname: Qian
authors.forename: Qiang
authors.initials: Q
authors.lastname: Xia
mesh_terms: D000818:Animals; D001013:Aorta, Thoracic / Q000503:physiopathology*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D006439:Hemodynamics / Q000187:drug effects*; D007191:Indazoles / Q000494:pharmacology; D008297:Male; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012772:Shock, Septic / Q000503:physiopathology*
chemical_list: D004791:Enzyme Inhibitors; D007191:Indazoles; D019001:Nitric Oxide Synthase; C080122:7-nitroindazole; D019331:NG-Nitroarginine Methyl Ester