title: Headspace stir bar sorptive extraction-gas chromatography/mass spectrometry characterization of the diluted vapor phase of cigarette smoke delivered to an in vitro cell exposure chamber.
abstract: Advanced smoke generation systems, such as the Borgwaldt RM20S(®) smoking machine used in combination with the BAT exposure chamber, allow for the generation, dilution and delivery of fresh cigarette smoke to cell or tissue cultures for in vitro cell culture analyses. Recently, our group confirmed that the Borgwaldt RM20S(®) is a reliable tool to generate and deliver repeatable and reproducible exposure concentrations of whole smoke to in vitro cultures. However, the relationship between dose and diluted smoke components found within the exposure chamber has not been characterized. The current study focused on the development of a headspace stir bar sorptive extraction (HSSE) method to chemically characterize some of the vapor phase components of cigarette smoke generated by the Borgwaldt RM20S(®) and collected within a cell culture exposure chamber. The method was based on passive sampling within the chamber by HSSE using a Twister™ stir bar. Following exposure, sorbed analytes were recovered using a thermal desorption unit and a cooled injection system coupled to gas chromatograph/mass spectrometry for identification and quantification. Using the HSSE method, sixteen compounds were identified. The desorption parameters were assessed using ten reference compounds and the following conditions led to the maximal response: desorption temperature of 200°C for 2 min with cryofocussing temperature of -75°C. During transfer of the stir bars to the thermal desorption system, significant losses of analytes were observed as a function of time; therefore, the exposure-to-desorption time interval was kept at the minimum of 10±0.5 min. Repeatability of the HSSE method was assessed by monitoring five reference compounds present in the vapor phase (10.1-12.9% RSD) and n-butyl acetate, the internal standard (18.5% RSD). The smoke dilution precision was found to be 17.2, 6.2 and 11.7% RSD for exposure concentrations of 1, 2 and 5% (v/v) cigarette vapor phase in air, respectively. A linear response of analyte abundance was observed as a function of dilution. Extrapolation to 100% (v/v) cigarette vapor phase, i.e., undiluted smoke, gave yields for the five compounds ranging from 6 to 450 ng for 10 min exposure.
authors.affiliation: Department of Chemistry, University of Montréal, C.P. 6128, succ. Centre-Ville, Montréal, Québec H3C 3J7, Canada.
authors.forename: Navneet
authors.initials: N
authors.lastname: Kaur
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Cabral
authors.forename: André
authors.initials: A
authors.lastname: Morin
authors.forename: Karen C
authors.initials: KC
authors.lastname: Waldron
mesh_terms: D000393:Air Pollutants / Q000032:analysis* / Q000302:isolation & purification; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods*; D005591:Chemical Fractionation / Q000379:methods*; D004781:Environmental Exposure / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006358:Hot Temperature; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014028:Tobacco Smoke Pollution / Q000032:analysis*
chemical_list: D000393:Air Pollutants; D014028:Tobacco Smoke Pollution