title: [Effects of oxygen-glucose deprivation on cultured rat hippocampal neurons].
abstract: OBJECTIVE
  To investigate the effects of oxygen-glucose deprivation on cultured rat hippocampal neurons.
  
  
  METHODS
  The hippocampal neurons cultured for 12 d were exposed to combined oxygen-glucose deprivation for 0.5 - 4 h and then cultured with original medium in normoxia for 28 h. Necrotic neurons were identified by 0.4% trypan blue staining and apoptotic neurons were detected by a TUNEL technique. Meanwhile, the area, perimeter and circle diameter of cell bodies were measured respectively by a photography analysis system.
  
  
  RESULTS
  The percentage of necrotic cells in cultured hippocampal neurons increased significantly during oxygen-glucose deprivation, but the percentage of apoptotic cells increased significantly after 28 h oxygen-glucose recovery. Photography analysis showed that area, perimeter and circle diameter of the necrotic cell bodies were larger than those of the apoptotic ones.
  
  
  CONCLUSIONS
  Oxygen-glucose deprivation can lead to severe damage of cultured hippocampal neurons. The necrosis is major during acute oxygen-glucose deprivation, while the apoptosis is major 28 h after oxygen-glucose recovery.
authors.affiliation: Department of Neurobiology, Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Bejing 100850, China.
authors.forename: Ai-Shi
authors.initials: AS
authors.lastname: Ding
authors.forename: Yah
authors.initials: Y
authors.lastname: Wu
authors.forename: Zi-Min
authors.initials: ZM
authors.lastname: Ma
authors.forename: Hai-Sheng
authors.initials: HS
authors.lastname: Li
authors.forename: Li-Ying
authors.initials: LY
authors.lastname: Wu
authors.forename: Ming
authors.initials: M
authors.lastname: Fan
mesh_terms: D000818:Animals; D015687:Cell Hypoxia; D002478:Cells, Cultured; D005947:Glucose / Q000172:deficiency*; D006624:Hippocampus / Q000166:cytology*; D009474:Neurons / Q000166:cytology*; D010100:Oxygen / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D005947:Glucose; D010100:Oxygen