title: Toward clinical proteomics on a next-generation sequencing platform.
abstract: We report the first next generation sequencing (NGS) application to identify and quantify proteins. Customization of protein specific aptamers enabled direct conversion of serum protein information into NGS read outs. The intrinsic ability of aptamer sequencing to highly multiplex protein detection and quantification, together with the prospect of DNA sequencing further evolving into a commodity technology, could constitute the core of a novel, universal diagnostics paradigm.
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Turner
authors.forename: Robin
authors.initials: R
authors.lastname: Tuytten
authors.forename: Kris P F
authors.initials: KP
authors.lastname: Janssen
authors.forename: Jeroen
authors.initials: J
authors.lastname: Lammertyn
authors.forename: Jan
authors.initials: J
authors.lastname: Wuyts
authors.forename: Jeroen
authors.initials: J
authors.lastname: Pollet
authors.forename: Sven
authors.initials: S
authors.lastname: Eyckerman
authors.forename: Clive
authors.initials: C
authors.lastname: Brown
authors.forename: Koen
authors.initials: K
authors.lastname: Kas
mesh_terms: D030562:Databases, Protein; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood; D040901:Proteomics / Q000379:methods*; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D007073:Immunoglobulin E