title: [Culture and green fluorescent protein gene transfection of pulp cells from goat deciduous teeth in vitro].
abstract: OBJECTIVE
  To isolate and culture pulp cells from goat deciduous teeth, and transfect green fluorescent protein gene.
  
  
  METHODS
  Pulp cells from goat deciduous teeth were obtained by tissue culture. Cell growth curve was measured by counting the number of cells, HE and alkaline phophatase(AKP) stain, as well as immunhistochemical stain of vimentin and keratin were performed. Virus supernatant was used to infect cell green fluorescent protein gene.
  
  
  RESULTS
  For the pulp cells, the cell group double time was 43.79 hours. AKP stain and immunhistochemical stain of vimentin were both positive, while immunhistochemical stain of keratin was negative. The infected cells expressed green fluorescent.
  
  
  CONCLUSIONS
  Pulp cells could be cultured from goat deciduous teeth, and express green fluorescent successfully.
authors.affiliation: Department of Stomatology, The Sixth People's Hospital, Shanghai Jiaotong University, Shanghai 200233, China.
authors.forename: Jia-yu
authors.initials: JY
authors.lastname: Lu
authors.forename: Li
authors.initials: L
authors.lastname: Hua
authors.forename: Xiu-li
authors.initials: XL
authors.lastname: Zhang
authors.forename: De-rong
authors.initials: DR
authors.lastname: Zou
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D003782:Dental Pulp; D006041:Goats; D049452:Green Fluorescent Proteins; D066298:In Vitro Techniques; D014094:Tooth, Deciduous; D014162:Transfection
chemical_list: D049452:Green Fluorescent Proteins