title: [Protection mechanisms of ATP-sensitive K channels on hippocampal CA1 neurons during chronic severe hypoxia].
abstract: OBJECTIVE
  To study the protection mechanisms of K(ATP) channels on hippocampal CA1 neurons during chronic severe hypoxia.
  
  
  METHODS
  p53 expression, DNA fraction, and cell apoptosis were examined in cultured hippocampal neurons in control group, hypoxia group, hypoxia group treated with K(ATP) channels antagonist and hypoxia group treated with K(ATP) channels agonist.
  
  
  RESULTS
  In the group of a 12 h long exposure to oxygen concentration of 0%, diazoxide (100 micromol/L), the K(ATP) channels agonist, reduced p53 expression and the hypoxia-induced apoptosis. In contrast, tolbutamide (100 micromol/L), the K(ATP) channels antagonist, significantly rose p53 expression and the hypoxia-induced apoptosis, which could be reversed by p53 inhibitor TSA.
  
  
  CONCLUSIONS
  K(ATP) channels protect hippocampal neurons against chronic severe hypoxia by suppressing p53 expression.
authors.affiliation: School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515, China.
authors.forename: Lian-Yan
authors.initials: LY
authors.lastname: Huang
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Li
authors.forename: Bo-Xing
authors.initials: BX
authors.lastname: Li
authors.forename: Fei
authors.initials: F
authors.lastname: Zou
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D017209:Apoptosis; D015687:Cell Hypoxia; D002470:Cell Survival; D003981:Diazoxide / Q000494:pharmacology; D016158:Genes, p53; D006624:Hippocampus / Q000166:cytology* / Q000378:metabolism; D054086:KATP Channels / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D018408:Patch-Clamp Techniques; D051381:Rats; D017207:Rats, Sprague-Dawley; D014044:Tolbutamide / Q000494:pharmacology
chemical_list: D054086:KATP Channels; D000255:Adenosine Triphosphate; D014044:Tolbutamide; D003981:Diazoxide