title: Inclusion of the central exon of parvovirus B19 precursor mRNA is determined by multiple splicing enhancers in both the exon and the downstream intron.
abstract: Alternative splicing of the precursor mRNA (pre-mRNA) of human parvovirus B19 (B19V) plays a key role in posttranscriptional regulation of B19V gene expression. We report that the central exon of the B19V pre-mRNA is defined by three GAA motif-containing exonic splicing enhancers and a G/GU-rich intronic splicing enhancer that lies adjacent to the second donor site. Moreover, targeting of morpholino antisense oligonucleotides to the two splicing enhancers surrounding the second donor site led to a significant reduction in splicing at this donor site during B19V infection of permissive CD36(+) erythroid progenitor cells.
authors.affiliation: Department of Microbiology, Molecular Genetics and Immunology, Kansas City, KS 66160, USA.
authors.forename: Wuxiang
authors.initials: W
authors.lastname: Guan
authors.forename: Fang
authors.initials: F
authors.lastname: Cheng
authors.forename: Qinfeng
authors.initials: Q
authors.lastname: Huang
authors.forename: Steve
authors.initials: S
authors.lastname: Kleiboeker
authors.forename: Jianming
authors.initials: J
authors.lastname: Qiu
mesh_terms: D017398:Alternative Splicing; D001483:Base Sequence; D004742:Enhancer Elements, Genetic; D016731:Erythema Infectiosum / Q000821:virology; D015672:Erythroid Precursor Cells / Q000821:virology; D005091:Exons; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D016732:Parvovirus B19, Human / Q000235:genetics*; D012322:RNA Precursors / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics*
chemical_list: D012322:RNA Precursors; D012333:RNA, Messenger; D012367:RNA, Viral