title: Effects of pimecrolimus versus triamcinolone on Langerhans cells after UV exposure.
abstract: OBJECTIVE
  Pimecrolimus is a topical immunomodulator for atopic dermatitis. Concerns regarding malignancy risk resulted in its black box warning in 2006. The purpose of this study is to determine the effects of pimecrolimus on Langerhans cells (LC), mediators of the cutaneous immunity UV-irradiated skin.
  
  
  METHODS
  A RCT was conducted investigating pimecrolimus 1% cream vs triamcinolone 0.1% cream on UV-irradiated epidermal LC on 20 healthy volunteers. Punch biopsies were stained with antibodies to CD1a, HLADR and CD83.
  
  
  RESULTS
  Triamcinolone caused more depletion in UV-irradiated CD1a(+) epidermis relative to pimecrolimus treatment. (P=0.030). Using HLA-DR as a pan-marker for APCs, pimecrolimus caused marginally less depletion than triamcinolone (P=0.013). Using anti-CD83 as a maturation marker, UV-irradiated skin treated with pimecrolimus showed more mature LC than skin treated with triamcinolone (P=0.00090).
  
  
  CONCLUSIONS
  UV-induced changes in LC are minimally affected by pimecrolimus, compared with triamcinolone.
authors.forename: Kathryn J
authors.initials: KJ
authors.lastname: Martires
authors.forename: Diana S
authors.initials: DS
authors.lastname: Domingo
authors.forename: Andrew
authors.initials: A
authors.lastname: Hsia
authors.forename: Pingfu
authors.initials: P
authors.lastname: Fu
authors.forename: Kevin D
authors.initials: KD
authors.lastname: Cooper
authors.forename: Elma D
authors.initials: ED
authors.lastname: Baron
mesh_terms: D000293:Adolescent; D000328:Adult; D015703:Antigens, CD / Q000378:metabolism; D018949:Antigens, CD1 / Q000378:metabolism; D006684:HLA-DR Antigens / Q000378:metabolism; D006801:Humans; D007136:Immunoglobulins / Q000378:metabolism; D007801:Langerhans Cells / Q000187:drug effects* / Q000378:metabolism* / Q000528:radiation effects*; D008562:Membrane Glycoproteins / Q000378:metabolism; D008875:Middle Aged; D012867:Skin / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects; D016559:Tacrolimus / Q000031:analogs & derivatives* / Q000494:pharmacology; D014221:Triamcinolone / Q000493:pharmacokinetics*; D014466:Ultraviolet Rays; D055815:Young Adult; D000097802:CD83 Antigen
chemical_list: D015703:Antigens, CD; D018949:Antigens, CD1; D006684:HLA-DR Antigens; D007136:Immunoglobulins; D008562:Membrane Glycoproteins; D016559:Tacrolimus; D014221:Triamcinolone; D000097802:CD83 Antigen; C419393:CD1a antigen; C117268:pimecrolimus