title: [Effects of hypoxia on the secretions of proinflammatory cytokines TNF-alpha and IL-6 in macrophages and its mechanism].
abstract: OBJECTIVE
  To investigate the effects of hypoxia on the secretions of proinflammatory cytokines TNF-alpha and IL-6 and to inquire into the mechanism.
  
  
  METHODS
  Separated mice abdominal macrophages which were identified with non-specific esterase dye method, and created the hypoxic cultured model. The levels of TNF-alpha and IL-6 in the medium were determined by ELISA method. The mRNA expressions of TNF-alpha and IL-6 were measured by RT-PCR method. NF-kappaB activation was assayed by Western blot method. Finaly, we added cortone (5 microg/ml) to the medium, then observed the secretion levels of TNF-alpha and IL-6 during hypoxia.
  
  
  RESULTS
  The secretions of TNF-alpha and IL-6 from Mphi exposed to hypoxia for 12 h were increased significantly compared with control (P < 0.01). The expressions of TNF-alpha mRNA and IL-6 mRNA were enhanced obviously contrasted with control (P < 0.01). NF-kappaB activation in Mphi nuclei was raised at 2 h during hypoxia and persisted to 5 h. We added cortone to the medium and found no significant change in secretion of TNF-alpha and IL-6 during hypoxia.
  
  
  CONCLUSIONS
  Hypoxia could activate NF-kappaB and make it shift to nucleus which promoted the transcriptions and expressions of TNF-alpha and IL-6.
authors.affiliation: Institute of Health and Environmental Medicine, Academy of Military Medical Sciences, Tianjin 300050, China.
authors.forename: Cui-Ping
authors.initials: CP
authors.lastname: Zhang
authors.forename: Yin-Zhi
authors.initials: YZ
authors.lastname: Xie
authors.forename: Peng
authors.initials: P
authors.lastname: Chen
authors.forename: Xin
authors.initials: X
authors.lastname: Hong
authors.forename: Hong-Jing
authors.initials: HJ
authors.lastname: Nie
authors.forename: Hong-Bin
authors.initials: HB
authors.lastname: Dong
authors.forename: Zhao-Yun
authors.initials: ZY
authors.lastname: Yin
mesh_terms: D000818:Animals; D015687:Cell Hypoxia; D002478:Cells, Cultured; D015850:Interleukin-6 / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D016328:NF-kappa B / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: D015850:Interleukin-6; D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha; D010100:Oxygen