title: Characterization of six antibacterial response genes from the European corn borer (Ostrinia nubilalis) larval gut and their expression in response to bacterial challenge.
abstract: Six cDNAs encoding putative antibacterial response proteins were identified and characterized from the larval gut of the European corn borer (Ostrinia nubilalis). These antibacterial response proteins include four peptidoglycan recognition proteins (PGRPs), one β-1,3-glucanase-1 (βglu-1), and one lysozyme. Tissue-specific expression analysis showed that these genes were highly expressed in the midgut, except for lysozyme. Analysis of expression of these genes in different developmental stage showed that they were expressed in larval stages, but little or no detectable expression was found in egg, pupa and adult. When larvae were challenged with Gram-negative bacteria (Enterobacter aerogenes), the expression of all six genes was up-regulated in the fatbodies. However, when larvae were challenged with Gram-positive bacteria (Micrococcus luteus), only PGRP-C and lysozyme genes were up-regulated. This study provides additional insights into the expression of antibacterial response genes in O. nubilalis larvae and helps us better understand the immune defense response in O. nubilalis.
authors.affiliation: Department of Entomology, Kansas State University, Manhattan, KS 66506, USA.
authors.forename: Chitvan
authors.initials: C
authors.lastname: Khajuria
authors.forename: Lawrent L
authors.initials: LL
authors.lastname: Buschman
authors.forename: Ming-Shun
authors.initials: MS
authors.lastname: Chen
authors.forename: Ludek
authors.initials: L
authors.lastname: Zurek
authors.forename: Kun Yan
authors.initials: KY
authors.lastname: Zhu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000276:immunology; D021902:Enterobacter aerogenes / Q000276:immunology / Q000502:physiology*; D041981:Gastrointestinal Tract / Q000276:immunology / Q000382:microbiology; D005786:Gene Expression Regulation; D019476:Insect Proteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology*; D007814:Larva; D016982:Micrococcus luteus / Q000276:immunology / Q000502:physiology*; D008969:Molecular Sequence Data; D009036:Moths / Q000145:classification / Q000235:genetics / Q000276:immunology* / Q000382:microbiology*; D010802:Phylogeny; D016415:Sequence Alignment; D003313:Zea mays
chemical_list: D000900:Anti-Bacterial Agents; D019476:Insect Proteins