title: High-performance purification of quaternary alkaloids from Corydalis yanhusuo W. T. Wang using a new polar-copolymerized stationary phase.
abstract: An efficient method for purification of alkaloids from Corydalis yanhusuo W. T. Wang using HPLC was developed, featuring a polar-copolymerized stationary phase named C18HCE. As ionizable solutes, the crude alkaloid sample often suffered from serious peak tailing problem on conventional RP-LC columns, and the separation would rapidly became destroyed with the increasing of load amount. However, on the new stationary phase, good peak shapes (asymmetry factor <1.5) as well as good loadability were easily obtained in a commonly used acidic mobile phase condition. The loading amount could reach 10 mg per injection on an analytical C18HCE column for laboratory-scale purification. About 6.8 mg of palmatine (HPLC purity >98%) and 44.4 mg of dehydrocorydaline (HPLC purity >98%) were rapidly derived from 200 mg of the crude alkaloid sample, and the recoveries of these two compounds were 76.5 and 81.7%, respectively. The purified alkaloids were characterized by comparing retention times with standard compounds as well as (1)H-NMR data. The new method is simple and high yielding, and it may provide a promising tool for purification of alkaloids as well as other alkaline compounds.
authors.affiliation: Key Lab of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, P. R. China.
authors.forename: Chaoran
authors.initials: C
authors.lastname: Wang
authors.forename: Zhimou
authors.initials: Z
authors.lastname: Guo
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Jing
authors.initials: J
authors.lastname: Zeng
authors.forename: Xiuli
authors.initials: X
authors.lastname: Zhang
authors.forename: Xinmiao
authors.initials: X
authors.lastname: Liang
mesh_terms: D000470:Alkaloids / Q000737:chemistry / Q000302:isolation & purification*; D001600:Berberine Alkaloids / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods*; D031311:Corydalis / Q000737:chemistry*; D015394:Molecular Structure
chemical_list: D000470:Alkaloids; D001600:Berberine Alkaloids; C007232:dehydrocorydalin; C005413:palmatine