title: microRNA-92a promotes lymph node metastasis of human esophageal squamous cell carcinoma via E-cadherin.
abstract: microRNAs (miRNAs) regulate gene expression at the post-transcriptional level and play important roles in tumor initiation and progression. Recently, we examined the global miRNA expression profile of esophageal squamous cell carcinoma (ESCC) and demonstrated that miR-92a was highly expressed in tumor tissues. In this study, we found that the up-regulation of miR-92a was significantly correlated with the status of lymph node metastasis and TNM stage in 107 ESCC patients. Moreover, the up-regulation of miR-92a was associated with poor survival of ESCC patients and might be used as an independent prognostic factor. Next, we investigated the role and mechanism of miR-92a in ESCC cells, and found that miR-92a modulated the migration and invasion but not apoptosis and proliferation of ESCC cells in vitro. We further demonstrated that miR-92a directly targeted the CDH1 3'-UTR and repressed the expression of CDH1, a tumor metastasis suppressor. In addition, restoring of miR-92a-resistant CDH1 expression in miR-92a-overexpression cells recovered the pro-metastasis activity of miR-92a. Taken together, we demonstrated that miR-92a promotes ESCC cell migration and invasion at least partially via suppression of CDH1 expression, and patients with up-regulated miR-92a are prone to lymph node metastasis and thus have poor prognosis.
authors.affiliation: Department of Thoracic Surgery, Cancer Institute and Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100021, China.
authors.forename: Zhao-li
authors.initials: ZL
authors.lastname: Chen
authors.forename: Xiao-hong
authors.initials: XH
authors.lastname: Zhao
authors.forename: Ji-wen
authors.initials: JW
authors.lastname: Wang
authors.forename: Bao-zhong
authors.initials: BZ
authors.lastname: Li
authors.forename: Zhen
authors.initials: Z
authors.lastname: Wang
authors.forename: Jian
authors.initials: J
authors.lastname: Sun
authors.forename: Feng-wei
authors.initials: FW
authors.lastname: Tan
authors.forename: Da-peng
authors.initials: DP
authors.lastname: Ding
authors.forename: Xiao-hui
authors.initials: XH
authors.lastname: Xu
authors.forename: Fang
authors.initials: F
authors.lastname: Zhou
authors.forename: Xiao-gang
authors.initials: XG
authors.lastname: Tan
authors.forename: Jie
authors.initials: J
authors.lastname: Hang
authors.forename: Su-sheng
authors.initials: SS
authors.lastname: Shi
authors.forename: Xiao-li
authors.initials: XL
authors.lastname: Feng
authors.forename: Jie
authors.initials: J
authors.lastname: He
mesh_terms: D015703:Antigens, CD; D015820:Cadherins / Q000096:biosynthesis*; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D002465:Cell Movement; D004938:Esophageal Neoplasms / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008198:Lymph Nodes / Q000378:metabolism* / Q000473:pathology; D008207:Lymphatic Metastasis; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis*; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D012334:RNA, Neoplasm / Q000096:biosynthesis*; D015996:Survival Rate; D014407:Tumor Cells, Cultured
chemical_list: D015703:Antigens, CD; C513384:CDH1 protein, human; D015820:Cadherins; C541874:MIRN92 microRNA, human; D035683:MicroRNAs; D012334:RNA, Neoplasm