title: Thrombin expression in prostate: a novel finding.
abstract: BACKGROUND
  A variable repertoire of coagulation protein expression is observed in different cancers. We evaluated expression of thrombin in prostate tissue.
  
  
  METHODS
  Detection of thrombin was performed using quantitative real-time PCR in fresh tissue and in situ hybridization (ISH) in archival prostate tissue and by immunohistochemistry of prostate tissue microarrays.
  
  
  RESULTS
  (Pro)thrombin mRNA expression was detected in cancer tissue and localized to prostatic epithelium and stroma by ISH. Thrombin protein was detected in stroma of benign and malignant epithelium (p <.05).
  
  
  CONCLUSIONS
  Prostate tissue is a rich reservoir of thrombin. This may have potential for developing antithrombin-based cancer therapy.
authors.affiliation: Department of Oncology, Mayo Clinic, Rochester, Minnesota 55905, USA. kohli.manish@mayo.edu
authors.forename: Manish
authors.initials: M
authors.lastname: Kohli
authors.forename: Karin
authors.initials: K
authors.lastname: Williams
authors.forename: Jorge L
authors.initials: JL
authors.lastname: Yao
authors.forename: Richard A
authors.initials: RA
authors.lastname: Dennis
authors.forename: Jiaoti
authors.initials: J
authors.lastname: Huang
authors.forename: Jay
authors.initials: J
authors.lastname: Reeder
authors.forename: William A
authors.initials: WA
authors.lastname: Ricke
mesh_terms: D004847:Epithelial Cells / Q000737:chemistry; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D016133:Polymerase Chain Reaction; D011467:Prostate / Q000737:chemistry* / Q000473:pathology; D011471:Prostatic Neoplasms / Q000737:chemistry* / Q000235:genetics / Q000473:pathology; D011516:Prothrombin / Q000032:analysis* / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D017154:Stromal Cells / Q000737:chemistry; D013917:Thrombin / Q000032:analysis* / Q000235:genetics; D046888:Tissue Array Analysis
chemical_list: D012333:RNA, Messenger; D011516:Prothrombin; D013917:Thrombin