title: [Effect of beta-adrenoceptor on NO-induced attenuation in spontaneous contractions of ileum in mice].
abstract: OBJECTIVE
  To investigate the influence of L-arginine (NO donors, L-Arg) on spontaneous contractions of ileum in mice and study the effects of activation of beta-adrenoceptor on NO-induced inhibition in spontaneous contractions of ileum.
  
  
  METHODS
  The method of spontaneous contractions recording was used to investigate the effect of L-NNA, ODQ, Isoprenaline( beta-adrenoceptor agonist) and Propranolol (beta-adrenoceptor antagonist) on NO-induced inhibition in spontaneous contractions of ileum.
  
  
  RESULTS
  (1) L-Arg inhibited the spontaneous contractions of ileum and had concentration-response relationship. (2) L-NNA (3 x 10(-4) mol/L), ODQ (3 x 10(-6) mol/L) relieved the inhibitory effect of L-Arg in ileum . (3) Propronalol (3 x 10(-6) mol/L) decreased significantly the inhibitory effect of L-Arg. (4) Iso (1 x 10(-7) mol/L) increased the inhibitory effect of L-Arg. After Iso (1 x 10(-7) mol/L) and Propronalol (3 x 10(-6) mol/L) being coapplied, the inhibitory effect of L-Arg was not changed.
  
  
  CONCLUSIONS
  NOS catalyzed L-Arg and produced NO. NO exerted its inhibitory effect by the cGMP pathway, the activation of beta-adrenoceptor was partly involved in NO-induced relaxation in ileum.
authors.affiliation: Department of Physiology, TongJi Medical College, HuaZhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Li-Qun
authors.initials: LQ
authors.lastname: Ma
authors.forename: Huan-Zhong
authors.initials: HZ
authors.lastname: Hu
authors.forename: Qin
authors.initials: Q
authors.lastname: Tian
authors.forename: Chang-Dong
authors.initials: CD
authors.lastname: Wang
authors.forename: Xiao-Min
authors.initials: XM
authors.lastname: Wang
authors.forename: Cheng-Gao
authors.initials: CG
authors.lastname: Yu
mesh_terms: D000818:Animals; D001120:Arginine / Q000494:pharmacology*; D007082:Ileum / Q000502:physiology*; D051379:Mice; D008815:Mice, Inbred Strains; D009119:Muscle Contraction / Q000502:physiology*; D009569:Nitric Oxide / Q000096:biosynthesis*; D011943:Receptors, Adrenergic, beta / Q000378:metabolism*
chemical_list: D011943:Receptors, Adrenergic, beta; D009569:Nitric Oxide; D001120:Arginine