title: Design of a novel flow-and-shoot microbeam.
abstract: Presented here is a novel microbeam technology--the Flow-And-ShooT (FAST) microbeam--under development at RARAF. In this system, cells undergo controlled fluidic transport along a microfluidic channel intersecting the microbeam path. They are imaged and tracked in real-time, using a high-speed camera and dynamically targeted, using a magnetic Point and Shoot system. With the proposed FAST system, RARAF expects to reach a throughput of 100,000 cells per hour, which will allow increasing the throughput of experiments by at least one order of magnitude. The implementation of FAST will also allow the irradiation of non-adherent cells (e.g. lymphocytes), which is of great interest to many of the RARAF users. This study presents the design of a FAST microbeam and results of first tests of imaging and tracking as well as a discussion of the achievable throughput.
authors.affiliation: RARAF, Columbia University, 136 S. Broadway, Irvington, NY 10533, USA. gyg2101@columbia.edu
authors.forename: G
authors.initials: G
authors.lastname: Garty
authors.forename: M
authors.initials: M
authors.lastname: Grad
authors.forename: B K
authors.initials: BK
authors.lastname: Jones
authors.forename: Y
authors.initials: Y
authors.lastname: Xu
authors.forename: J
authors.initials: J
authors.lastname: Xu
authors.forename: G
authors.initials: G
authors.lastname: Randers-Pehrson
authors.forename: D
authors.initials: D
authors.lastname: Attinger
authors.forename: D J
authors.initials: DJ
authors.lastname: Brenner
mesh_terms: D018929:Cell Culture Techniques / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D011853:Radiobiology / Q000295:instrumentation* / Q000379:methods*; D013673:Technology Assessment, Biomedical; D014916:Whole-Body Irradiation / Q000295:instrumentation* / Q000379:methods*