title: [Construction of underexpression HSP90alpha and overexpression HSP90beta human hepatoma cell line HepG2].
abstract: OBJECTIVE
  To construct underexpression HSP90alpha and overexpression HSP90beta human hepatoma cell line HepG2.
  
  
  METHODS
  The combined plasimid pSilencerHSP90alpha and pSmycHSP90beta were introduced into HepG2 by electroporation, respectively. The result of transfection was identified by Western-blotting and the curve of cell growth was drew by MTT. Observe the cell vitality and expression of HSP90.
  
  
  RESULTS
  Expression of HSP90 in transfected cell line was shown by Western-blotting: Compared with control, expression of HSP90 in the cells transfected with pSilencerHSP90alpha decreased, whereas that in the cells transfected with pSmycHSP90beta increased.The growth curves of the two groups of transfected cells was as the same as that of the control group.
  
  
  CONCLUSIONS
  The stable overexpression HSP90beta and underexpression HSP90alpha HepG2 cell lines were established.
authors.affiliation: Chinese PLA Institute of Disease Prevention and Control, Beijing 100071, China.
authors.forename: Xue-lin
authors.initials: XL
authors.lastname: Han
authors.forename: Xue-mei
authors.initials: XM
authors.lastname: Chen
authors.forename: Fei
authors.initials: F
authors.lastname: Zou
mesh_terms: D001483:Base Sequence; D018274:Electroporation; D018841:HSP90 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D056945:Hep G2 Cells / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics; D014162:Transfection
chemical_list: D018841:HSP90 Heat-Shock Proteins; C533585:HSP90AA2P protein, human; C493165:HSP90AB1 protein, human; D012333:RNA, Messenger; D034741:RNA, Small Interfering