title: Biophysical characterization of DNA and RNA aptamer interactions with hen egg lysozyme.
abstract: This work characterized the binding of an RNA aptamer recognizing hen egg white lysozyme, as well as a literature-reported single-stranded DNA analog of sequence identical to the original RNA aptamer, using fluorescence anisotropy, isothermal titration calorimetry (ITC) and analytical ultracentrifugation. The polyanionic DNA aptamer analog is selective for lysozyme even over cationic cytochrome c and has been reported to be successfully used in biosensing applications. The association however, is predominantly of electrostatic character, strongly salt-sensitive and entropically-driven, in contrast to previously described enthalpically-driven antibody-lysozyme and DNA aptamer-VEGF interactions. With a moderate selectivity for their target, high salt-sensitivity along with fast association and dissociation behavior, these molecules might serve as pseudo-affinity ligands for biomolecular separations.
authors.affiliation: Department of Chemical & Biomolecular Engineering, University of Houston, 4800 Calhoun Rd, Houston, TX 77204-4004, USA.
authors.forename: Ajish S R
authors.initials: AS
authors.lastname: Potty
authors.forename: Katerina
authors.initials: K
authors.lastname: Kourentzi
authors.forename: Han
authors.initials: H
authors.lastname: Fang
authors.forename: Peter
authors.initials: P
authors.lastname: Schuck
authors.forename: Richard C
authors.initials: RC
authors.lastname: Willson
mesh_terms: D000818:Animals; D052157:Aptamers, Nucleotide / Q000737:chemistry / Q000378:metabolism*; D001483:Base Sequence; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009113:Muramidase / Q000378:metabolism*; D009994:Osmolar Concentration; D011485:Protein Binding; D013696:Temperature
chemical_list: D052157:Aptamers, Nucleotide; C421864:hen egg lysozyme; D009113:Muramidase