title: [Analysis of mitochondrial DNA D-loop region mutation and its significance in human oncocytoma].
abstract: OBJECTIVE
  To investigate the mutation in mitochondrial DNA displacement-loop (mtDNA D-loop) region in oncocytoma and its relationship with tumorigenesis and tumor development.
  
  
  METHODS
  The mtDNA D-Loop region of 20 thyroid or renal oncocytomas and the adjacent normal tissues were amplified by PCR, and then sequenced. Five human fetal renal tissues were collected as matched controls.
  
  
  RESULTS
  Among the 20 oncocytomas, 21 mutations which focused on hypervariable region I (HVI) were found in 7 tumor tissues and 1 normal tissue with the mutation rates of 35% and 5%, respectively. At the same time, 191 polymorphisms were found in the 20 cases.
  
  
  CONCLUSIONS
  mtDNA D-loop region, especially HV I, is the mutational hotspot of oncocytomas, which may be closely related with mtDNA duplicating rate and the function of mitochondria.
authors.affiliation: Department of Pathology, Tianjin Medical University, Tianjin 300070, China. songwenjing2004@yahoo.com.cn
authors.forename: Wen-jing
authors.initials: WJ
authors.lastname: Song
authors.forename: Li-min
authors.initials: LM
authors.lastname: Yan
authors.forename: Xiu-lan
authors.initials: XL
authors.lastname: Zhao
authors.forename: Zeng-hui
authors.initials: ZH
authors.lastname: Liu
authors.forename: Bao-cun
authors.initials: BC
authors.lastname: Sun
mesh_terms: D018249:Adenoma, Oxyphilic / Q000235:genetics*; D001483:Base Sequence; D004252:DNA Mutational Analysis; D004272:DNA, Mitochondrial / Q000235:genetics*; D005260:Female; D006801:Humans; D007680:Kidney Neoplasms / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D008928:Mitochondria / Q000235:genetics; D009154:Mutation; D011110:Polymorphism, Genetic; D013964:Thyroid Neoplasms / Q000235:genetics*
chemical_list: D004272:DNA, Mitochondrial