title: Bioactivity of American ginseng by knockout extract preparation using monoclonal antibody.
abstract: We produced anti-ginsenoside Re (GRe)-4G10 monoclonal antibody that specifically recognizes GRe and used this to prepare an immunoaffinity column to remove GRe from American ginseng berry extract (AGBE) (thus producing GRe knock-out AGBE (GRe-KO-AGBE)). We compared the anti-diabetic and anti-obesity effects of AGBE and GRe-KO-AGBE in adult diabetic C57BL/6J ob/ob mice. Fasting blood glucose levels and body weight were measured in mice receiving daily intraperitoneal injections of 150 mg/kg of either extract for 27 consecutive days. On Day 6, the group taking AGBE had significantly lower fasting blood glucose levels compared to GRe-KO-AGBE-treated mice (70.5 mg/dl vs. 140 mg/dl). On Day 13, the glucose levels in both groups were 201 mg/dl for AGBE-treated group and 169 mg/dl for GRe-KO-AGBE, respectively. GRe (20 mg/kg) had dose-related effects on fasting blood glucose levels (reduced to 188 and 180 mg/dl on Day 5 and Day 12, respectively, compared to 229 and 235 mg/dl, respectively, in the vehicle group). This hypoglycemic effect persisted even after treatment discontinued (blood glucose levels at 3 days post-treatment: 198 and 253 mg/dl with ginsenoside and vehicle treatment, respectively). The monoclonal antibody technique can be used to prepare botanical knockout extracts for bioactivity studies.
authors.affiliation: Tang Center for Herbal Medicine Research, The Pritzker School of Medicine, The University of Chicago, 5841 S. Maryland Avenue, MC 4028, Chicago, IL 60637, USA. CYuan@airway2.bsd.uchicago.edu
authors.forename: Chun-Su
authors.initials: CS
authors.lastname: Yuan
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Tanaka
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000378:metabolism; D001786:Blood Glucose / Q000187:drug effects*; D001835:Body Weight / Q000187:drug effects; D003924:Diabetes Mellitus, Type 2 / Q000188:drug therapy*; D005638:Fruit; D015870:Gene Expression; D036145:Ginsenosides / Q000032:analysis / Q000235:genetics / Q000276:immunology* / Q000494:pharmacology; D005951:Glucose Tolerance Test; D007004:Hypoglycemic Agents / Q000032:analysis / Q000494:pharmacology; D007274:Injections, Intraperitoneal; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008820:Mice, Obese; D009765:Obesity / Q000188:drug therapy; D005894:Panax; D008517:Phytotherapy; D010936:Plant Extracts / Q000032:analysis / Q000235:genetics / Q000276:immunology* / Q000494:pharmacology
chemical_list: D000911:Antibodies, Monoclonal; D001786:Blood Glucose; D036145:Ginsenosides; D007004:Hypoglycemic Agents; D010936:Plant Extracts; C049864:ginsenoside Re