title: Synthesis, in vitro and in vivo preliminary evaluation of anti-angiogenic properties of some pyrroloazaflavones.
abstract: This work investigated the in vitro and in vivo anti-angiogenic activity of some pyrroloazaflavones, exactly 2-phenyl-1H-pyrrolo[2,3-h]quinolin-4(7H)ones, with vinblastine as reference compound. Growth inhibitory activity, migration, and capillary-like structures formation were determined in human umbilical vein endothelial cell cultures, and Matrigel plug assay was carried out to evaluate in vivo effects on angiogenesis. Collectively, our results indicate that some pyrroloazaflavone derivatives, at non-cytotoxic concentrations and like vinblastine are able: (i) to exert in vitro anti-angiogenic activity and (ii) to counteract in vitro and in vivo the pro-angiogenic effects of fibroblast growth factor-2 (FGF-2).
authors.affiliation: Department of Pharmaceutical Sciences, University of Padova, via F. Marzolo, 5, 35131 Padova, Italy. mariagrazia.ferlin@unipd.it
authors.forename: Maria Grazia
authors.initials: MG
authors.lastname: Ferlin
authors.forename: Maria Teresa
authors.initials: MT
authors.lastname: Conconi
authors.forename: Luca
authors.initials: L
authors.lastname: Urbani
authors.forename: Barbara
authors.initials: B
authors.lastname: Oselladore
authors.forename: Diego
authors.initials: D
authors.lastname: Guidolin
authors.forename: Rosa
authors.initials: R
authors.lastname: Di Liddo
authors.forename: Pier Paolo
authors.initials: PP
authors.lastname: Parnigotto
mesh_terms: D020533:Angiogenesis Inhibitors / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001372:Aza Compounds / Q000737:chemistry; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects; D047309:Flavones / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008810:Mice, Inbred C57BL; D011758:Pyrroles / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D020533:Angiogenesis Inhibitors; D001372:Aza Compounds; D047309:Flavones; D011758:Pyrroles