title: Gramella gaetbulicola sp. nov., a member of the family Flavobacteriaceae isolated from foreshore soil.
abstract: A Gram-reaction-negative, yellow-pigmented, gliding, rod-shaped, aerobic bacterium (RA5-111(T)) was isolated from foreshore soil. The taxonomic status of the novel isolate was determined using a polyphasic approach. On the basis of 16S rRNA gene sequence similarities, strain RA5-111(T) could be assigned to the genus Gramella, with sequence similarities of 97.7, 97.3 and 96.2 % to the type strains of Gramella echinicola, Gramella portivictoriae and Gramella marina, respectively. Chemotaxonomic and phenotypic characteristics also supported the affiliation of strain RA5-111(T) with the genus Gramella. The genomic DNA G+C content was 39.1 mol%. The isolate contained MK-6 as the predominant menaquinone, iso-C(15 : 0), iso-C(17 : 0) 3-OH and a summed feature (iso-C(15 : 0) 2-OH and/or C(16 : 1)ω7c) as major fatty acids, and phosphatidylethanolamine and unknown phospholipids as the polar lipids. DNA-DNA relatedness, phenotypic, genotypic and chemotaxonomic data clearly indicate that the isolate represents a novel species of the genus Gramella, for which the name Gramella gaetbulicola sp. nov. is proposed. The type strain is RA5-111(T) ( = KCTC 23022(T) = JCM 16528(T) = NBRC 106272(T)).
authors.affiliation: Department of Microbiology and Molecular Biology, School of Bioscience and Biotechnology, Chungnam National University, 220 Gung-dong, Yuseong, Daejeon 305-764, Republic of Korea.
authors.forename: Sung-Heun
authors.initials: SH
authors.lastname: Cho
authors.affiliation: Department of Microbiology and Molecular Biology, School of Bioscience and Biotechnology, Chungnam National University, 220 Gung-dong, Yuseong, Daejeon 305-764, Republic of Korea.
authors.forename: Song-Hee
authors.initials: SH
authors.lastname: Chae
authors.affiliation: Department of Microbiology and Molecular Biology, School of Bioscience and Biotechnology, Chungnam National University, 220 Gung-dong, Yuseong, Daejeon 305-764, Republic of Korea.
authors.forename: Myoungho
authors.initials: M
authors.lastname: Cho
authors.affiliation: Department of Microbiology and Molecular Biology, School of Bioscience and Biotechnology, Chungnam National University, 220 Gung-dong, Yuseong, Daejeon 305-764, Republic of Korea.
authors.forename: Tae-Ui
authors.initials: TU
authors.lastname: Kim
authors.affiliation: Department of Microbiology and Molecular Biology, School of Bioscience and Biotechnology, Chungnam National University, 220 Gung-dong, Yuseong, Daejeon 305-764, Republic of Korea.
authors.forename: Seri
authors.initials: S
authors.lastname: Choi
authors.affiliation: Department of Microbiology and Molecular Biology, School of Bioscience and Biotechnology, Chungnam National University, 220 Gung-dong, Yuseong, Daejeon 305-764, Republic of Korea.
authors.forename: Ji-Hye
authors.initials: JH
authors.lastname: Han
authors.affiliation: Department of Microbiology and Molecular Biology, School of Bioscience and Biotechnology, Chungnam National University, 220 Gung-dong, Yuseong, Daejeon 305-764, Republic of Korea.
authors.forename: Young Tae
authors.initials: YT
authors.lastname: Kim
authors.affiliation: Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies, San 89 Wangsan-ri, Cheoin, Yongin, Gyeonggi 449-791, Republic of Korea.
authors.forename: Yochan
authors.initials: Y
authors.lastname: Joung
authors.affiliation: Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies, San 89 Wangsan-ri, Cheoin, Yongin, Gyeonggi 449-791, Republic of Korea.
authors.forename: Kiseong
authors.initials: K
authors.lastname: Joh
authors.affiliation: Pacific Institute of Bioorganic Chemistry of the Far-Eastern Branch of the Russian Academy of Sciences, Prospekt 100 Let Vladivostoku 159, Vladivostok 690022, Russia.
authors.forename: Olga I
authors.initials: OI
authors.lastname: Nedashkovskaya
authors.affiliation: Department of Microbiology and Molecular Biology, School of Bioscience and Biotechnology, Chungnam National University, 220 Gung-dong, Yuseong, Daejeon 305-764, Republic of Korea.
authors.forename: Seung Bum
authors.initials: SB
authors.lastname: Kim
mesh_terms: D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000378:metabolism; D041965:Flavobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S