title: Lactobacillus-mediated priming of the respiratory mucosa protects against lethal pneumovirus infection.
abstract: The inflammatory response to respiratory virus infection can be complex and refractory to standard therapy. Lactobacilli, when targeted to the respiratory epithelium, are highly effective at suppressing virus-induced inflammation and protecting against lethal disease. Specifically, wild-type mice primed via intranasal inoculation with live or heat-inactivated Lactobacillus plantarum or Lactobacillus reuteri were completely protected against lethal infection with the virulent rodent pathogen, pneumonia virus of mice; significant protection (60% survival) persisted for at least 13 wk. Protection was not unique to Lactobacillus species, and it was also observed in response to priming with nonpathogenic Gram-positive Listeria innocua. Priming with live lactobacilli resulted in diminished granulocyte recruitment, diminished expression of multiple proinflammatory cytokines (CXCL10, CXCL1, CCL2, and TNF), and reduced virus recovery, although we have demonstrated clearly that absolute virus titer does not predict clinical outcome. Lactobacillus priming also resulted in prolonged survival and protection against the lethal sequelae of pneumonia virus of mice infection in MyD88 gene-deleted (MyD88(-/-)) mice, suggesting that the protective mechanisms may be TLR-independent. Most intriguing, virus recovery and cytokine expression patterns in Lactobacillus-primed MyD88(-/-) mice were indistinguishable from those observed in control-primed MyD88(-/-) counterparts. In summary, we have identified and characterized an effective Lactobacillus-mediated innate immune shield, which may ultimately serve as critical and long-term protection against infection in the absence of specific antiviral vaccines.
authors.affiliation: Eosinophil Biology Section, Laboratory of Allergic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
authors.forename: Stanislaw J
authors.initials: SJ
authors.lastname: Gabryszewski
authors.forename: Ofir
authors.initials: O
authors.lastname: Bachar
authors.forename: Kimberly D
authors.initials: KD
authors.lastname: Dyer
authors.forename: Caroline M
authors.initials: CM
authors.lastname: Percopo
authors.forename: Kristin E
authors.initials: KE
authors.lastname: Killoran
authors.forename: Joseph B
authors.initials: JB
authors.lastname: Domachowske
authors.forename: Helene F
authors.initials: HF
authors.lastname: Rosenberg
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D000956:Antigens, Viral / Q000378:metabolism; D016207:Cytokines / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D018836:Inflammation Mediators / Q000037:antagonists & inhibitors / Q000378:metabolism; D048191:Lactiplantibacillus plantarum / Q000276:immunology*; D052200:Limosilactobacillus reuteri / Q000276:immunology*; D008168:Lung / Q000276:immunology / Q000473:pathology / Q000821:virology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D029122:Murine pneumonia virus / Q000276:immunology* / Q000472:pathogenicity; D018186:Pneumovirus Infections / Q000276:immunology / Q000401:mortality* / Q000517:prevention & control*; D020545:Respiratory Mucosa / Q000276:immunology* / Q000382:microbiology* / Q000821:virology; D014779:Virus Replication / Q000276:immunology
chemical_list: D000956:Antigens, Viral; D016207:Cytokines; D018836:Inflammation Mediators