title: Fast GC-MS method for the simultaneous screening of THC-COOH, cocaine, opiates and analogues including buprenorphine and fentanyl, and their metabolites in urine.
abstract: A fast gas chromatography (GC)-MS method has been developed and validated for the simultaneous screening of different classes of drugs of abuse in urine. Tetrahydrocannabinol metabolite, cocaine, opiates such as morphine, O-6-monoacetylmorphine (O-6-MAM), codeine, opioids such as buprenorphine, methadone, pentazocine, fentanyl and analogues and their main metabolites can be detected and quantified after a simple liquid-liquid extraction in alkaline conditions and derivatisation to obtain the corresponding trimethylsilyl derivatives. The chromatographic separation is performed in a total time of 6 min, using a short GC column (5% phenyl methyl silicone, 10-m length × 0.18-mm internal diameter). The Limits of Detection are satisfactory for forensic purposes for all the substances; the repeatability of concentrations (percent coefficients of variation) are always lower than 15% at high and low concentration levels, and accuracy, intended as % error on the true value, is always lower than 15% for all the analytes. The method can successfully be applied for screening analyses in many fields of forensic toxicology.
authors.affiliation: Laboratorio Antidoping, FMSI, L.go G. Onesti 1, 00197, Rome, Italy. Sabina.stranorossi@gmail.com
authors.forename: Sabina
authors.initials: S
authors.lastname: Strano-Rossi
authors.forename: Ana Maria
authors.initials: AM
authors.lastname: Bermejo
authors.forename: Xavier
authors.initials: X
authors.lastname: de la Torre
authors.forename: Francesco
authors.initials: F
authors.lastname: Botrè
mesh_terms: D002047:Buprenorphine / Q000378:metabolism / Q000652:urine*; D003042:Cocaine / Q000378:metabolism / Q000652:urine*; D013759:Dronabinol / Q000031:analogs & derivatives* / Q000378:metabolism / Q000652:urine; D005283:Fentanyl / Q000378:metabolism / Q000652:urine*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans
chemical_list: D002047:Buprenorphine; D013759:Dronabinol; C025351:delta(9)-tetrahydrocannabinolic acid; D003042:Cocaine; D005283:Fentanyl