title: Diphlorethohydroxycarmalol, isolated from the brown algae Ishige okamurae, protects against radiation-induced cell damage in mice.
abstract: The aim of this study was to evaluate the radioprotective effects of diphlorethohydroxycarmalol (DPHC), isolated from the brown algae Ishige okamurae, in mice subjected to gamma irradiation. DPHC significantly decreased the level of radiation-induced intracellular reactive oxygen species in cultured Chinese hamster lung fibroblast (V79-4) cells (p < 0.05), enhanced cell viability that decreased after exposure to γ-rays, and reduced radiation-induced apoptosis in the V79-4 cells. Pretreatment with DPHC (100 mg/kg) in mice prior to irradiation significantly protected the intestinal crypt cells in the jejunum (p < 0.01) and maintained villi height (p < 0.01), compared with those of the vehicle-treated irradiated group. Mice pretreated with DPHC also exhibited dose-dependent increases in the bone marrow cell viability. The dose-reduction factor for gamma irradiation in the DPHC-pretreated mice was 2.05 at 3.5 days after irradiation. These results suggest that DHPC plays a role in protecting cells from irradiation-induced apoptosis, through the scavenging of reactive oxygen species in vitro, and that DPHC significantly protected intestinal progenitor cells and bone marrows cells that were decreased by gamma irradiation in vivo.
authors.affiliation: Department of Anatomy, College of Medicine, Jeju National University, Jeju 690-756, South Korea.
authors.forename: Meejung
authors.initials: M
authors.lastname: Ahn
authors.forename: Changjong
authors.initials: C
authors.lastname: Moon
authors.forename: Wonjun
authors.initials: W
authors.lastname: Yang
authors.forename: Eun-Ju
authors.initials: EJ
authors.lastname: Ko
authors.forename: Jin Won
authors.initials: JW
authors.lastname: Hyun
authors.forename: Hong Gu
authors.initials: HG
authors.lastname: Joo
authors.forename: Youngheun
authors.initials: Y
authors.lastname: Jee
authors.forename: Nam Ho
authors.initials: NH
authors.lastname: Lee
authors.forename: Jae Woo
authors.initials: JW
authors.lastname: Park
authors.forename: Ryeo Kyeong
authors.initials: RK
authors.lastname: Ko
authors.forename: Gi Ok
authors.initials: GO
authors.lastname: Kim
authors.forename: Taekyun
authors.initials: T
authors.lastname: Shin
mesh_terms: D000818:Animals; D006224:Cricetinae; D003412:Cricetulus; D005720:Gamma Rays / Q000009:adverse effects*; D006575:Heterocyclic Compounds, 3-Ring / Q000302:isolation & purification / Q000494:pharmacology*; D051379:Mice; D000459:Phaeophyceae / Q000737:chemistry*; D011837:Radiation-Protective Agents / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D006575:Heterocyclic Compounds, 3-Ring; D011837:Radiation-Protective Agents; C513811:diphlorethohydroxycarmalol