title: Geometrical separation method for lipoproteins using bioformulated-fiber matrix electrophoresis: size of high-density lipoprotein does not reflect its density.
abstract: The increasing number of patients with metabolic syndrome is a critical global problem. In this study, we describe a novel geometrical electrophoretic separation method using a bioformulated-fiber matrix to analyze high-density lipoprotein (HDL) particles. HDL particles are generally considered to be a beneficial component of the cholesterol fraction. Conventional electrophoresis is widely used but is not necessarily suitable for analyzing HDL particles. Furthermore, a higher HDL density is generally believed to correlate with a smaller particle size. Here, we use a novel geometrical separation technique incorporating recently developed nanotechnology (Nata de Coco) to contradict this belief. A dyslipidemia patient given a 1-month treatment of fenofibrate showed an inverse relationship between HDL density and size. Direct microscopic observation and morphological observation of fractionated HDL particles confirmed a lack of relationship between particle density and size. This new technique may improve diagnostic accuracy and medical treatment for lipid related diseases.
authors.affiliation: Department of Chemistry, Faculty of Science, Rikkyo University, Tokyo, Japan. mtabuchi@rikkyo.ac.jp
authors.forename: Mari
authors.initials: M
authors.lastname: Tabuchi
authors.forename: Makoto
authors.initials: M
authors.lastname: Seo
authors.forename: Takayuki
authors.initials: T
authors.lastname: Inoue
authors.forename: Takeshi
authors.initials: T
authors.lastname: Ikeda
authors.forename: Akinori
authors.initials: A
authors.lastname: Kogure
authors.forename: Ikuo
authors.initials: I
authors.lastname: Inoue
authors.forename: Shigehiro
authors.initials: S
authors.lastname: Katayama
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Matsunaga
authors.forename: Akira
authors.initials: A
authors.lastname: Hara
authors.forename: Tsugikazu
authors.initials: T
authors.lastname: Komoda
mesh_terms: D000091:Acetobacter / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D008075:Lipoproteins, HDL / Q000032:analysis*; D036103:Nanotechnology / Q000379:methods*; D010316:Particle Size
chemical_list: D008075:Lipoproteins, HDL