title: Aging and its impact on the quality of grafts: an experimental study in rats livers.
abstract: BACKGROUND
  There are consistent clues of worse results with grafts from older donors, especially in hepatitis C. University of Wisconsin (UW) solution is adopted for liver preservation, but other solutions are being studied, as fructose-1,6-bisphosphate (FBP).
  
  
  OBJECTIVE
  To determinate the impact of aging of the donor on the cold ischemia injury in rat livers and compare UW and FBP.
  
  
  METHODS
  Twenty male Wistar rats were studied - 10, aging 20 weeks: 5 to preservation with UW (C-UW) and 5, FBP (C-FBP); and other 10, aging 50 weeks: 5 to UW (E-UW) and 5 to FBP (E-FBP). Rats were anesthesized, submitted to hepatectomy, and graft was kept under 2-4ºC for 8 hours. Liquid samples were taken at 2, 4, 6, and 8 hours, to determine AST and LDH. At the end, in the liver tissue thiobarbituric acid reactive substances and catalase were determined.
  
  
  RESULTS
  Average liver weight was similar between controls and the others (12.5 g ± 1.8 and 13.4 g ± 2.0). AST and LDH levels were higher in C-UW group (P<0.05). In the older group, there was a difference between UW and FBP preserved livers related to LDH, but not to AST. Thiobarbituric acid reactive substances were superior in control group than in the older one (P = 0.001). Catalase activity was similar between these groups (P = 0.11), but it was superior in UW preserved animals (P = 0.02).
  
  
  CONCLUSIONS
  Livers from older rats were similar to the controls regarding cold ischemia injury in FBP group. Surprisingly, with UW solution there was less cold ischemia injury in the elderly group. When comparing both solutions, FBP provided significantly more protection than UW in the controls. There was a trend to FBP to being better than UW in the elderly group. Further studies with liver from older donors and ischemia and reperfusion are needed.
authors.affiliation: Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brasil. smmota@terra.com.br
authors.forename: Stela Maria
authors.initials: SM
authors.lastname: Mota
authors.forename: Glauber
authors.initials: G
authors.lastname: Gasperin
authors.forename: Carlos Thadeu
authors.initials: CT
authors.lastname: Cerski
authors.forename: Jarbas Rodrigues de
authors.initials: JR
authors.lastname: Oliveira
authors.forename: Mário Reis
authors.initials: MR
authors.lastname: Alvares-da-Silva
mesh_terms: D000241:Adenosine; D000367:Age Factors; D000493:Allopurinol; D000818:Animals; D015925:Cryopreservation / Q000379:methods; D005635:Fructosediphosphates; D005978:Glutathione; D007328:Insulin; D008099:Liver; D008297:Male; D009926:Organ Preservation / Q000379:methods*; D019188:Organ Preservation Solutions; D011887:Raffinose; D051381:Rats; D017208:Rats, Wistar
chemical_list: D005635:Fructosediphosphates; D007328:Insulin; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; D005978:Glutathione; D000241:Adenosine; C029063:fructose-1,6-diphosphate; D011887:Raffinose