title: Differential proteomic analysis of temperature-induced autolysis in mycelium of Pleurotus tuber-regium.
abstract: Autolysis is an important physiological process found in fungal cultivation. However, there is hitherto no report on the autolysis of Pleurotus tuber-regium. We have investigated the enzymes secreted by temperature-induced (40°C as treatment versus 10°C as control) autolysis of the mycelium of P. tuber-regium grown in submerged cultivation. A comparison between the intracellular proteins (inside the mycelium) and the extracellular proteins (in the culture medium) of the treatment and control by proteomic analysis involving 2D PAGE and MALDI-TOF-MS was made. Twenty-two up-regulated protein spots were detected and eight proteins were identified. They included proteasome which participates in the ubiquitin-proteasome pathway; β-1,3-glucanosyltransferase and tubulin which are involved in the renewal and repair of cell wall; protease and endoglucanase which promote the natural degradation of cell wall and cytoplasm; 14-3-3 protein which takes part in cell signal transduction; and two putative proteins presumably relate to the autolysis process. These identified proteins suggest partially the metabolic processes of the autolysis in the P. tuber-regium mycelium.
authors.affiliation: School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
authors.forename: Bifang
authors.initials: B
authors.lastname: Huang
authors.forename: Wenxiong
authors.initials: W
authors.lastname: Lin
authors.forename: Peter C K
authors.initials: PC
authors.lastname: Cheung
authors.forename: Jinzhong
authors.initials: J
authors.lastname: Wu
mesh_terms: D015180:Electrophoresis, Gel, Two-Dimensional; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D008969:Molecular Sequence Data; D025282:Mycelium / Q000737:chemistry / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D020076:Pleurotus / Q000737:chemistry* / Q000166:cytology* / Q000235:genetics / Q000378:metabolism; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013696:Temperature
chemical_list: D005656:Fungal Proteins