title: A Zn2+-specific turn-on fluorescent probe for ratiometric sensing of pyrophosphate in both water and blood serum.
abstract: A novel fluorescent sensor composed of a naphthalene functionalized tetraazamacrocycle ligand 3,6,9,15-tetraazabicyclo[9.3.1]pentadeca-1(15),11,13-triene-3-methyl naphthalene (1) and Zn(2+) has been designed and prepared, which can be utilized for selective and ratiometric sensing of pyrophosphate (PPi) over other phosphate-containing anions in aqueous solution at physiological pH. Notably, the water soluble 1 itself also exhibits a selective enhanced fluorescent response to Zn(2+), and the complex 1-Zn(2+) thus formed eventually fulfils the synergic Zn(2+) coordination-altered strategy with PPi. Furthermore, the ratiometric sensing of 1-Zn(2+) towards PPi performed well even in blood serum milieu. Finally, the sensor 1-Zn(2+) was successfully employed to monitor a real-time assay of inorganic pyrophosphatase (PPase) by means of ratiometric fluorescent measurements for the first time.
authors.affiliation: State Key Laboratory of Coordination Chemistry, Coordination Chemistry Institute, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, PR China.
authors.forename: Jinghan
authors.initials: J
authors.lastname: Wen
authors.forename: Zhirong
authors.initials: Z
authors.lastname: Geng
authors.forename: Yuxin
authors.initials: Y
authors.lastname: Yin
authors.forename: Zhong
authors.initials: Z
authors.lastname: Zhang
authors.forename: Zhilin
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000838:Anions / Q000737:chemistry; D015374:Biosensing Techniques; D002413:Cations, Divalent / Q000737:chemistry; D002712:Chlorides / Q000737:chemistry; D011756:Diphosphates / Q000032:analysis / Q000097:blood* / Q000737:chemistry*; D057075:Enzyme Assays / Q000379:methods; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D043564:Inorganic Pyrophosphatase / Q000737:chemistry; D008024:Ligands; D015394:Molecular Structure; D009942:Organometallic Compounds / Q000138:chemical synthesis* / Q000737:chemistry*; D044967:Serum / Q000737:chemistry; D012996:Solutions / Q000737:chemistry; D014867:Water / Q000737:chemistry; D015032:Zinc / Q000737:chemistry*; D017967:Zinc Compounds / Q000737:chemistry
chemical_list: D000838:Anions; D002413:Cations, Divalent; D002712:Chlorides; D011756:Diphosphates; D005456:Fluorescent Dyes; D008024:Ligands; D009942:Organometallic Compounds; D012996:Solutions; D017967:Zinc Compounds; D014867:Water; C016837:zinc chloride; D043564:Inorganic Pyrophosphatase; D015032:Zinc