title: Use of serum circulating CCNB2 in cancer surveillance.
abstract: Cyclin B2 (CCNB2), a member of the cyclin protein family, has been found to be up-regulated in human cancers. To evaluate the potential use of circulating CCNB2 in serum in cancer surveillance, we examined relative expression levels of serum circulating CCNB2 mRNA in 103 cancer patients, 19 normal controls, and 40 benign disease patients using real-time quantitative reverse transcriptase polymerase chain reaction. We found that the relative expression level of circulating CCNB2 mRNA in cancer patients was significantly higher (p<0.0001) than that in normal controls and benign diseases group. Circulating CCNB2 mRNA level was significantly (p<0.001) correlated with cancer stage and metastasis status. Receiver operating characteristic (ROC) analysis showed an area under the curve (AUC) of 0.87 and 0.83 (p<0.05) in identifying cancer patients' metastasis status in lung and digestive tract cancer, respectively. Moreover, we observed that expression levels of circulating CCNB2 mRNA in cancer patients significantly decreased (p=0.0084) after their therapeutic treatments. These data suggest that detection of serum circulating CCNB2 mRNA may have potential clinical applications in screening and monitoring of metastasis and therapeutic treatments.
authors.affiliation: School of Life Sciences, Tsinghua University, Beijing, PR China.
authors.forename: Min-Li
authors.initials: ML
authors.lastname: Mo
authors.forename: Zhao
authors.initials: Z
authors.lastname: Chen
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.forename: Hai-Long
authors.initials: HL
authors.lastname: Li
authors.forename: Qing
authors.initials: Q
authors.lastname: Sheng
authors.forename: Hui-Yan
authors.initials: HY
authors.lastname: Ma
authors.forename: Fang-Xin
authors.initials: FX
authors.lastname: Zhang
authors.forename: Ya-Wei
authors.initials: YW
authors.lastname: Hua
authors.forename: Xun
authors.initials: X
authors.lastname: Zhang
authors.forename: Da-Qiang
authors.initials: DQ
authors.lastname: Sun
authors.forename: Mei-Lin
authors.initials: ML
authors.lastname: Xu
authors.forename: Run-Gui
authors.initials: RG
authors.lastname: Niu
authors.forename: Jun-Pu
authors.initials: JP
authors.lastname: Xu
authors.forename: Hui-Jing
authors.initials: HJ
authors.lastname: Zuo
authors.forename: Min
authors.initials: M
authors.lastname: Liu
authors.forename: Hai-Meng
authors.initials: HM
authors.lastname: Zhou
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood*; D016022:Case-Control Studies; D056765:Cyclin B2 / Q000097:blood* / Q000235:genetics; D004067:Digestive System Neoplasms / Q000175:diagnosis / Q000473:pathology; D005260:Female; D005833:Genital Neoplasms, Female / Q000175:diagnosis / Q000473:pathology; D006801:Humans; D008175:Lung Neoplasms / Q000175:diagnosis / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009369:Neoplasms / Q000175:diagnosis* / Q000473:pathology; D012333:RNA, Messenger / Q000097:blood*; D015533:Transcriptional Activation; D001749:Urinary Bladder Neoplasms / Q000175:diagnosis / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; D056765:Cyclin B2; D012333:RNA, Messenger