title: Brainstem Hap1-Ahi1 is involved in insulin-mediated feeding control.
abstract: The function of the brainstem Hap1-Ahi1 complex in the regulation of feeding behavior was investigated. When mice were fasted or treated with 2-deoxy-D-glucose (2-DG), Hap1-Ahi1 was significantly upregulated. By using streptozotocin (STZ) to decrease the circulating insulin in mice, Hap1-Ahi1 was significantly increased. Furthermore, intra-brain injection of insulin decreased the expression of Hap1-Ahi1 in the brainstem. Moreover, when we knocked down the expression of brainstem Hap1 by RNAi, the mice showed decreased food intake and lower body weights. Collectively, our results indicate that the Hap1-Ahi1 complex in the brainstem works as a sensor for insulin signals in feeding control.
authors.affiliation: CAS Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, People's Republic of China.
authors.forename: Shao-Na
authors.initials: SN
authors.lastname: Niu
authors.forename: Zhen-Bo
authors.initials: ZB
authors.lastname: Huang
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
authors.forename: Xiu-Rong
authors.initials: XR
authors.lastname: Rao
authors.forename: Hui
authors.initials: H
authors.lastname: Kong
authors.forename: Jing
authors.initials: J
authors.lastname: Xu
authors.forename: Xiao-Jiang
authors.initials: XJ
authors.lastname: Li
authors.forename: Chuan
authors.initials: C
authors.lastname: Yang
authors.forename: Guo-Qing
authors.initials: GQ
authors.lastname: Sheng
mesh_terms: D033942:Adaptor Proteins, Vesicular Transport; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D015153:Blotting, Western; D001933:Brain Stem / Q000187:drug effects / Q000378:metabolism*; D045744:Cell Line, Tumor; D003847:Deoxyglucose / Q000494:pharmacology; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000235:genetics / Q000378:metabolism; D004435:Eating / Q000187:drug effects*; D005215:Fasting; D005786:Gene Expression Regulation / Q000187:drug effects; D007004:Hypoglycemic Agents / Q000494:pharmacology; D007150:Immunohistochemistry; D007328:Insulin / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D046912:Multiprotein Complexes / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017552:Solitary Nucleus / Q000378:metabolism
chemical_list: D033942:Adaptor Proteins, Vesicular Transport; C465818:Ahi1 protein, mouse; D001786:Blood Glucose; C490758:Hap1 protein, mouse; D007004:Hypoglycemic Agents; D007328:Insulin; D046912:Multiprotein Complexes; D009419:Nerve Tissue Proteins; D011518:Proto-Oncogene Proteins; D003847:Deoxyglucose