title: Improvement of the transformation efficiency of Flammulina velutipes Fv-1 using the glyceraldehyde-3-phosphate dehydrogenase gene promoter.
abstract: To improve the expression level of heterologous genes in Flammulina velutipes Fv-1, we constructed new vectors having glyceraldehydes-3-phosphate dehydrogenase (gpd) gene promoter to control the expression of target genes. When the hygromycin B phosphotransferase (hph) gene from Escherichia coli was controlled by the gpd promoter, transformation efficiency was 3-fold higher than the case of that controlled by the tryptophan synthetase gene (trp1) promoter.
authors.affiliation: Food Biotechnology Division, National Food Research Institute, Tsukuba, Ibaraki, Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Maehara
authors.forename: Shizuko
authors.initials: S
authors.lastname: Tomita
authors.forename: Koji
authors.initials: K
authors.lastname: Takabatake
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kaneko
mesh_terms: D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D055393:Flammulina / Q000235:genetics*; D005818:Genetic Engineering / Q000379:methods*; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics*; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics*; D014170:Transformation, Genetic / Q000235:genetics*
chemical_list: D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D017853:Phosphotransferases (Alcohol Group Acceptor); C063242:hygromycin-B kinase