title: Investigation of electrophoretic exclusion method for the concentration and differentiation of proteins.
abstract: This work presents a technique termed as "electrophoretic exclusion" that is capable of differentiation and concentration of proteins in bulk solution. In this method, a hydrodynamic flow is countered by the electrophoretic velocity to prevent a species from entering into a channel. The separation can be controlled by changing the flow rate or applied electric potential in order to exclude a certain species selectively while allowing others to pass through the capillary. The exclusion of various proteins is investigated using a flow-injection regime of the method. Concentration of myoglobin of up to 1200 times the background concentration in 60 s was demonstrated. Additionally, negatively charged myoglobin was separated from a solution containing negatively charged allophycocyanin. Cationic cytochrome c was also differentiated from a solution with allophycocyanin. The ability to differentially transport species in bulk solution enables parallel and serial separation modes not available with other separations schemes.
authors.affiliation: Department of Chemistry and Biochemistry, Arizona State University, Tempe, Arizona 85287, United States.
authors.forename: Michelle M
authors.initials: MM
authors.lastname: Meighan
authors.forename: Jared
authors.initials: J
authors.lastname: Vasquez
authors.forename: Luke
authors.initials: L
authors.lastname: Dziubcynski
authors.forename: Sarah
authors.initials: S
authors.lastname: Hews
authors.forename: Mark A
authors.initials: MA
authors.lastname: Hayes
mesh_terms: D004583:Electrons; D004586:Electrophoresis / Q000295:instrumentation / Q000379:methods*; D057230:Limit of Detection; D011506:Proteins / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D011506:Proteins