title: TRX is up-regulated by fibroblast growth factor-2 in lung carcinoma.
abstract: We have previously shown that exogenous fibroblast growth factor-2 (FGF-2) inhibits apoptosis of the small-cell lung cancer (SCLC) cell line NCI-H446, but the underlying mechanism remains unknown. In this study, the protein profiles of FGF-2-treated and untreated NCI-H446 cells were determined by 2-D gel electrophoresis combined with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and bioinformatics. Differential expression analysis of the protein profiles after FGF-2 treatment identified a total of 24 protein spots, of which nine were up-regulated and 15 were down-regulated. Four proteins were identified by MALDI-TOF-MS: thioredoxin (TRX), visfatin, ubiquitin carboxyl-terminal hydrolase L1 (UCHL1) and Cu/Zn superoxide dismutase (CuZn-SOD). Western blotting revealed that TRX was up-regulated in NCI-H446 and A549 cells treated with FGF-2. Furthermore, immunohistochemical staining confirmed that both FGF-2 and TRX were overexpressed in lung cancer tissues and could be correlated with both lymph node metastasis and clinical stage. These data indicate that TRX may be involved in the FGF-2 signaling pathway.
authors.affiliation: Department of Pathology, XiangYa School of Medicine, Central South University, Changsha, Hunan Province, China.
authors.forename: Zheng-Hao
authors.initials: ZH
authors.lastname: Deng
authors.forename: Hui-Qiu
authors.initials: HQ
authors.lastname: Cao
authors.forename: Yong-Bin
authors.initials: YB
authors.lastname: Hu
authors.forename: Ji-Fang
authors.initials: JF
authors.lastname: Wen
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Zhou
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism*; D000328:Adult; D015153:Blotting, Western; D045744:Cell Line, Tumor; D016207:Cytokines / Q000096:biosynthesis / Q000235:genetics; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D005786:Gene Expression Regulation; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D054409:Nicotinamide Phosphoribosyltransferase / Q000096:biosynthesis / Q000235:genetics; D055752:Small Cell Lung Carcinoma / Q000235:genetics / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013482:Superoxide Dismutase / Q000096:biosynthesis / Q000235:genetics; D013879:Thioredoxins / Q000096:biosynthesis* / Q000235:genetics; D043222:Ubiquitin Thiolesterase / Q000096:biosynthesis / Q000235:genetics; D015854:Up-Regulation
chemical_list: D016207:Cytokines; C482860:UCHL1 protein, human; D016222:Fibroblast Growth Factor 2; D013879:Thioredoxins; D013482:Superoxide Dismutase; D054409:Nicotinamide Phosphoribosyltransferase; C085455:nicotinamide phosphoribosyltransferase, human; D043222:Ubiquitin Thiolesterase