title: Possible defect of T suppressor cell subpopulation in patients with kidney acute rejection.
abstract: CD8(+)CD28(-) forkhead box P3 (Foxp3(+)) T suppressor (Ts) lymphocytes are antigen-specific cells capable of inducing tolerogenic antigen-presenting cells by up-regulation of inhibitory receptors immunoglobulin-like-transcripts -3 and -4 and down-regulation of costimulatory molecules. Our study sought to investigate the relation between the level of peripheral CD8(+)CD28(-)Foxp3(+) Ts cells and kidney allograft outcomes. The project included 44 kidney transplantation patients. During the 6-month period following transplantation an acute rejection episode (ARE) was diagnosed in 11 patients based on biopsy results using the Banff criteria. Peripheral blood samples collected at 1 day before as well as 14 and 30 days after transplantation were tested for CD8(+)CD28(-)Foxp3(+) T cells by means of flow cytometry. Values were considered significant when P < .05. Cytometric analysis did not show significant differences between the groups in pretransplant levels of peripheral CD8(+)CD28(-)Foxp3(+) Ts cells (P > .05); however, the posttransplantation analysis showed a higher mean level of Ts cells in nonrejection (NONARE) versus acute rejection (ARE) patients (P < .0001). This observation suggested that dysfunction of CD8(+)CD28(-)Foxp3(+) Ts cells observed in ARE patients may contribute to these episodes. Interestingly, we observed similar results with respect to peripheral CD4(+)CD25(+)Foxp3(+) T regulatory cells in ARE patients, suggesting impairment of immunoregulatory mechanisms (especially within the inducible Foxp3 system) in this group, leading to acute renal allograft rejection episodes.
authors.affiliation: Medical University in Poznan, Poƶnan, Poland.
authors.forename: J
authors.initials: J
authors.lastname: Karczewski
authors.forename: M
authors.initials: M
authors.lastname: Karczewski
authors.forename: K
authors.initials: K
authors.lastname: Wiktorowicz
mesh_terms: D000328:Adult; D015703:Antigens, CD / Q000276:immunology; D005260:Female; D005434:Flow Cytometry; D006084:Graft Rejection / Q000276:immunology*; D006801:Humans; D016030:Kidney Transplantation / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D013601:T-Lymphocytes / Q000276:immunology*; D014184:Transplantation, Homologous
chemical_list: D015703:Antigens, CD