title: Rapid and sensitive detection of bovine coronavirus and group a bovine rotavirus from fecal samples by using one-step duplex RT-PCR assay.
abstract: Bovine coronavirus (BCoV) and group A bovine rotavirus (BRV) are two of major causes for neonatal calf diarrhea. In the present study, a one-step duplex RT-PCR was established to detect and differentiate BCoV and group A BRV from fecal samples. The sensitivity of this method for BCoV and group A BRV was 10 PFU/100 µl and 1 PFU/100 µl, respectively. Twenty-eight diarrhea fecal samples were detected with this method, the result showed that 2 samples were identified as co-infected with BCoV and group A BRV, 26 samples were group A BRV positive, and 2 samples were negative. It proved that this method is sensitive for clinical fecal samples and is worth applying to laboratory diagnosis for BCoV and group A BRV.
authors.affiliation: Department of Veterinary Microbiology, University of Miyazaki, Miyazaki, Japan.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhu
authors.forename: Jianbao
authors.initials: J
authors.lastname: Dong
authors.forename: Takeshi
authors.initials: T
authors.lastname: Haga
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Goto
authors.forename: Masuo
authors.initials: M
authors.lastname: Sueyoshi
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis / Q000821:virology*; D018352:Coronavirus Infections / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D017938:Coronavirus, Bovine / Q000145:classification / Q000302:isolation & purification*; D005243:Feces / Q000821:virology*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary*; D012401:Rotavirus / Q000145:classification / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D012680:Sensitivity and Specificity