title: EFEMP1 expression promotes angiogenesis and accelerates the growth of cervical cancer in vivo.
abstract: OBJECTIVE
  The study was to investigate the role of EFEMP1 in angiogenesis and growth of cervical carcinoma in vivo.
  
  
  METHODS
  Effects of EFEMP1 on proliferation of Hela cells and HUVECs, invasion of Hela cells and migration of HUVECs, and adhesion of Hela cells to HUVECs were evaluated by MTT, Transwell chamber assay and adhesion assay, respectively. EFEMP1 overexpression in Hela cells was achieved by stable EFEMP1 gene transfection into Hela cells by Lipofectamin™ 2000 and the effectiveness of transfection was verified with western-blotting. The effect of EFEMP1 transfection upon the VEGF expression of Hela cells was detected with ELISA. The nude mouse models bearing cervical cancer were established with Hela cells transfected with EFEMP1 gene to observe the role of EFEMP1 in angiogenesis and growth of cervical cancer in vivo. VEGF expression and microvascular density of cervical cancer tissues were detected with immunohistochemistry and CD34 labeling respectively to elucidate the pathway by which EFEMP1 influences the growth of cervical cancer.
  
  
  RESULTS
  Proliferation and invasion of Hela cells were promoted by the EFEMP1 protein. The EFEMP1 gene transfection into Hela cells was successful and EFEMP1 gene obtained stable high expression in Hela cells. Compared to the control, the tumors with EFEMP1 overexpression showed a faster growth rate and had a higher level of VEGF expression and microvascular density. EFEMP1 gene transfection elevated the VEGF protein level in Hela cells and EFEMP1 protein facilitated the adhesion of Hela cells to HUVECs. However, no direct effect of EFEMP1 was observed on proliferation, migration and tube formation of HUVECs.
  
  
  CONCLUSIONS
  EFEMP1 promoted the angiogenesis and accelerated the growth of cervical carcinoma in vivo through a VEGF up-regulation pathway.
authors.affiliation: Biomedical Engineering Center, Department of Anatomy, the Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, Guangdong 510080, China.
authors.forename: En-Lin
authors.initials: EL
authors.lastname: Song
authors.forename: Ya-Ping
authors.initials: YP
authors.lastname: Hou
authors.forename: Shen-Ping
authors.initials: SP
authors.lastname: Yu
authors.forename: Sheng-Guo
authors.initials: SG
authors.lastname: Chen
authors.forename: Jun-Ting
authors.initials: JT
authors.lastname: Huang
authors.forename: Tao
authors.initials: T
authors.lastname: Luo
authors.forename: Ling-Ping
authors.initials: LP
authors.lastname: Kong
authors.forename: Jie
authors.initials: J
authors.lastname: Xu
authors.forename: Hua-Qiao
authors.initials: HQ
authors.lastname: Wang
mesh_terms: D000818:Animals; D048708:Cell Growth Processes / Q000187:drug effects / Q000502:physiology; D002465:Cell Movement / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects; D042783:Endothelial Cells / Q000166:cytology; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000494:pharmacology; D005260:Female; D006367:HeLa Cells; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009389:Neovascularization, Pathologic / Q000378:metabolism / Q000473:pathology; D011994:Recombinant Proteins / Q000494:pharmacology; D014162:Transfection; D014183:Transplantation, Heterologous; D002583:Uterine Cervical Neoplasms / Q000098:blood supply / Q000235:genetics / Q000378:metabolism* / Q000473:pathology
chemical_list: C119630:EFEMP1 protein, human; D016326:Extracellular Matrix Proteins; D011994:Recombinant Proteins