title: Characterizing the novel protein p33MONOX.
abstract: The novel protein p33MONOX (p33Monooxygenase) was over-expressed in neuroblastoma cells demonstrating its inhibitory effect on the phosphorylation of the App (amyloid precursor protein) and Bcl2 (B-cell lymphoma 2) proteins but mediating higher activation of Mapk1/3 (mitogen-activated protein kinase 1/3). We employed a variety of cell biology techniques to show the localization of p33MONOX to the cytoplasm of pyramidal neurons in the mouse brain hippocampus. We also carried out a yeast-two-hybrid screening plus co-immunoprecipitation and bio-informatics to determine COBRA1 (cofactor of BRCA1 (breast cancer type 1)), NOL12 (nucleolar protein 12), and PRNP (prion protein) as p33MONOX-interacting proteins. Bio-computational analyses revealed a flavine-containing monooxygenase (FMO)-1 motif, thus linking p33MONOX to a group of previously characterized proteins, the MICALs (molecule interacting with CasL). Concluding, p33MONOX might regulate pre- and post-transcriptional control of dynamic processes related to growth cone guidance.
authors.affiliation: Department of Molecular and Cell Biology, Nanyang Technological University, Singapore.
authors.forename: Manisha
authors.initials: M
authors.lastname: Mishra
authors.forename: Noriko
authors.initials: N
authors.lastname: Inoue
authors.forename: Klaus
authors.initials: K
authors.lastname: Heese
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001369:Axons / Q000378:metabolism / Q000502:physiology; D002478:Cells, Cultured; D003001:Cloning, Molecular; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D006899:Mixed Function Oxygenases / Q000235:genetics* / Q000302:isolation & purification* / Q000502:physiology; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000302:isolation & purification*; D016716:PC12 Cells; D017966:Pyramidal Cells / Q000378:metabolism / Q000502:physiology; D051381:Rats; D020539:Sequence Analysis, Protein; D014162:Transfection
chemical_list: D009419:Nerve Tissue Proteins; C559322:KIAA1191 protein, human; C559321:LOC306766 protein, rat; D006899:Mixed Function Oxygenases; C559323:p33MONOX protein, mouse