title: Identification and functional characterization of phenylglycine biosynthetic genes involved in pristinamycin biosynthesis in Streptomyces pristinaespiralis.
abstract: Pristinamycin I (PI), a streptogramin type B antibiotic produced by Streptomyces pristinaespiralis, contains the aproteinogenic amino acid L-phenylglycine. Recent sequence analysis led to the identification of a set of putative phenylglycine biosynthetic genes. Successive inactivation of the individual genes resulted in a loss of PI production. Production was restored by supplementation with externally added L-phenylglycine, which demonstrates that these genes are involved in phenylglycine biosynthesis and thus probably disclosing the last essential pristinamycin biosynthetic genes. Finally, a putative pathway for phenylglycine synthesis is proposed.
authors.affiliation: Mikrobiologie/Biotechnologie, Interfakultäres Institut für Mikrobiologie und Infektionsmedizin, Fakultät für Biologie, Eberhard Karls Universität Tübingen, Auf der Morgenstelle 28, D-72076 Tübingen, Germany. yvonne.mast@biotech.uni-tuebingen.de
authors.forename: Yvonne J
authors.initials: YJ
authors.lastname: Mast
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Wohlleben
authors.forename: Eva
authors.initials: E
authors.lastname: Schinko
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D003198:Computer Simulation; D005798:Genes, Bacterial; D005998:Glycine / Q000031:analogs & derivatives* / Q000096:biosynthesis; D053858:Metabolic Networks and Pathways; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D025762:Pristinamycin / Q000096:biosynthesis*; D013302:Streptomyces / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D025762:Pristinamycin; C008852:2-phenylglycine; D005998:Glycine