title: Development of a rapid detection method to detect tdh gene in Vibrio parahaemolyticus using 2-step ultrarapid real-time polymerase chain reaction.
abstract: Thermostable direct hemolysin encoded by tdh gene has been considered an important virulence factor in pathogenic Vibrio parahaemolyticus. Two-step ultrarapid real-time polymerase chain reaction (URRT PCR) with a microchip was devised to detect V. parahaemolyticus carrying tdh gene. This novel method has a 6-μL reaction volume and extremely reduces running time since one cycle can be completed in 10 s or less. Consequently, 35 cycles of URRT PCR was successfully able to detect up to 100 fg (18 copies) of genomic DNA from pathogenic V. parahaemolyticus carrying tdh gene in 6 min. These results indicate that this method is at present the most rapid detection method for tdh gene and pathogenic V. parahaemolyticus.
authors.affiliation: Department of Life Science, College of Natural Science, Kyonggi University, Suwon, South Korea.
authors.forename: Min-Hee
authors.initials: MH
authors.lastname: Kang
authors.forename: Il-Wook
authors.initials: IW
authors.lastname: Kim
authors.forename: Dong-Woo
authors.initials: DW
authors.lastname: Lee
authors.forename: Mi-Sun
authors.initials: MS
authors.lastname: Yoo
authors.forename: Sang-Hoon
authors.initials: SH
authors.lastname: Han
authors.forename: Byoung-Su
authors.initials: BS
authors.lastname: Yoon
mesh_terms: D001427:Bacterial Toxins / Q000235:genetics*; D001431:Bacteriological Techniques / Q000379:methods*; D006460:Hemolysin Proteins / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction / Q000379:methods*; D013997:Time Factors; D014736:Vibrio parahaemolyticus / Q000235:genetics / Q000472:pathogenicity*; D037521:Virulence Factors / Q000235:genetics*
chemical_list: D001427:Bacterial Toxins; D006460:Hemolysin Proteins; D037521:Virulence Factors; C068489:thermostable direct hemolysin