title: Streptomyces sundarbansensis sp. nov., an actinomycete that produces 2-allyloxyphenol.
abstract: A novel actinomycete producing 2-allyloxyphenol, designated strain MS1/7(T), was isolated from sediments of the Sundarbans mangrove forest, India. Growth on International Streptomyces Project (ISP) media 2, 3, 4, 5 and 7 produced olive green to grey aerial hyphae that carried smooth-surfaced spores in a flexuous (Rectiflexibiles) arrangement. The strain contained ll-diaminopimelic acid, but no diagnostic sugars in whole-cell hydrolysates. Hexa-, octa- and a minor amount of tetra-hydrogenated menaquinones with nine isoprene units [MK-9 (H(4), H(6), H(8) and H(10))] were present as isoprene analogues. Diagnostic phospholipids were phosphatidylethanolamine and diphosphatidylglycerol. The predominant fatty acids were anteiso-C(15 : 0) (34.80 %), iso-C(16 : 0) (16.45 %), C(16) (10.53 %) and anteiso-C(17 : 0) (10.92 %). The strain showed greater than 99 % 16S rRNA gene sequence similarity to the type strains of several recognized species of the genus Streptomyces, but in the phylogenetic tree based on 16S rRNA gene sequences it formed a distinct phyletic line and demonstrated closest relationships to viomycin-producers (Streptomyces californicus NRRL B-1221(T), Streptomyces floridae MTCC 2534(T) and Streptomyces puniceus NRRL B-2895(T)). However, strain MS1/7(T) could be distinguished from these and other closely related species based on low levels of DNA-DNA relatedness (<44 %) and disparate physiological features, principally amino acid utilization and growth in NaCl. Strain MS1/7(T) is therefore suggested to represent a novel species of the genus Streptomyces, for which the name Streptomyces sundarbansensis sp. nov. is proposed. The type strain is MS1/7(T) ( = MTCC 10621(T) = DSM 42019(T)).
authors.affiliation: School of Environmental Studies, Jadavpur University, Kolkata 700 032, India.
authors.forename: Meyyappan
authors.initials: M
authors.lastname: Arumugam
authors.affiliation: School of Environmental Studies, Jadavpur University, Kolkata 700 032, India.
authors.forename: Anindita
authors.initials: A
authors.lastname: Mitra
authors.affiliation: School of Environmental Studies, Jadavpur University, Kolkata 700 032, India.
authors.forename: Arnab
authors.initials: A
authors.lastname: Pramanik
authors.affiliation: School of Environmental Studies, Jadavpur University, Kolkata 700 032, India.
authors.forename: Malay
authors.initials: M
authors.lastname: Saha
authors.affiliation: Department of Life Science and Biotechnology, Jadavpur University, Kolkata 700 032, India.
authors.forename: Ratan
authors.initials: R
authors.lastname: Gachhui
authors.affiliation: School of Environmental Studies, Jadavpur University, Kolkata 700 032, India.
authors.forename: Joydeep
authors.initials: J
authors.lastname: Mukherjee
mesh_terms: D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000378:metabolism; D019015:Geologic Sediments / Q000382:microbiology*; D007194:India; D008969:Molecular Sequence Data; D010636:Phenols / Q000378:metabolism*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013302:Streptomyces / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010636:Phenols; D012336:RNA, Ribosomal, 16S