title: Anti-invasive activities of anthocyanins through modulation of tight junctions and suppression of matrix metalloproteinase activities in HCT-116 human colon carcinoma cells.
abstract: Claudins are a family of proteins that are the most important components of the tight junctions. Recently it has been reported that these proteins are overexpressed in cancers and there is a positive correlation between suppression of the expression of these proteins and anti-invasive activity. Matrix metalloproteinases (MMPs) have been implicated as important mediators in cancer invasion. Here, we investigated the effects of anthocyanins on tight junctions (TJs) and the expression of claudins as well as MMPs. The inhibitory effects of the anthocyanins on cell proliferation, motility and invasiveness were found to be associated with tightening TJs, which was demonstrated by an increase in transepithelial electrical resistance (TER). The expression of claudin proteins was suppressed by anthocyanins. Furthermore, the activities of MMP-2 and -9 were dose-dependently suppressed by anthocyanin treatment. These effects were related to activation of 38-MAPK and suppression of the PI3K/Akt pathway in HCT-116 human colon cancer cells.
authors.affiliation: Department of Biochemistry, College of Oriental Medicine, and Research Institute of Oriental Medicine, Busan, Republic of Korea.
authors.forename: Dong Yeok
authors.initials: DY
authors.lastname: Shin
authors.forename: Jing Nan
authors.initials: JN
authors.lastname: Lu
authors.forename: Gi-Young
authors.initials: GY
authors.lastname: Kim
authors.forename: Jin Myung
authors.initials: JM
authors.lastname: Jung
authors.forename: Ho Sung
authors.initials: HS
authors.lastname: Kang
authors.forename: Won Sup
authors.initials: WS
authors.lastname: Lee
authors.forename: Yung Hyun
authors.initials: YH
authors.lastname: Choi
mesh_terms: D000872:Anthocyanins / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D000970:Antineoplastic Agents / Q000494:pharmacology / Q000627:therapeutic use; D002277:Carcinoma / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D003110:Colonic Neoplasms / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical; D017097:Electric Impedance; D045325:HCT116 Cells; D006801:Humans; D061965:Matrix Metalloproteinase Inhibitors; D020782:Matrix Metalloproteinases / Q000378:metabolism; D009361:Neoplasm Invasiveness; D010936:Plant Extracts / Q000494:pharmacology; D019108:Tight Junctions / Q000187:drug effects* / Q000378:metabolism; D027843:Vitis / Q000737:chemistry
chemical_list: D000872:Anthocyanins; D000970:Antineoplastic Agents; D061965:Matrix Metalloproteinase Inhibitors; D010936:Plant Extracts; D020782:Matrix Metalloproteinases