title: Carbosilane dendrimers NN8 and NN16 form a stable complex with siGAG1.
abstract: A new mechanism of gene expression inhibition has been discovered as RNA interference, in which the ability of double-stranded RNA to stimulate specific degradation of an mRNA target with a complementary sequence to one of the double-stranded RNA strands. Water-soluble carbosilane dendrimers containing ammonium or amine groups at their periphery are biocompatible molecules that may be good candidates as non-viral carriers of small interfering RNA. In studying the formation of complex between anti-HIV siRNA siGAG1 and carbosilane dendrimers NN8 and NN16 by circular dichroism, fluorescence, and zeta-potential, the size of nanoparticles formed has been estimated by dynamic light scattering. At a charge ratio of 1:3-4 (siGAG1:dendrimer), the dendriplexes formed were in the size range of 250-350 nm.
authors.affiliation: Institute of Biophysics and Cell Engineering of NASB, Akademicheskaja 27, Minsk 220072, Belarus.
authors.forename: Dzmitry
authors.initials: D
authors.lastname: Shcharbin
authors.forename: Elzbieta
authors.initials: E
authors.lastname: Pedziwiatr
authors.forename: Olga
authors.initials: O
authors.lastname: Nowacka
authors.forename: Mayank
authors.initials: M
authors.lastname: Kumar
authors.forename: Marian
authors.initials: M
authors.lastname: Zaborski
authors.forename: Paula
authors.initials: P
authors.lastname: Ortega
authors.forename: F Javier
authors.initials: FJ
authors.lastname: de la Mata
authors.forename: Rafael
authors.initials: R
authors.lastname: Gómez
authors.forename: Ma Angeles
authors.initials: MA
authors.lastname: Muñoz-Fernandez
authors.forename: Maria
authors.initials: M
authors.lastname: Bryszewska
mesh_terms: D050091:Dendrimers / Q000737:chemistry*; D010316:Particle Size; D034741:RNA, Small Interfering / Q000737:chemistry*; D012821:Silanes / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D050091:Dendrimers; D034741:RNA, Small Interfering; D012821:Silanes; C504072:carbosilane