title: Histological approaches to study tissue parasitism during the experimental Trypanosoma cruzi infection.
abstract: During acute infection with the parasite Trypanosoma cruzi, the causal agent of Chagas' disease, tissue damage is related to intense tissue parasitism. Here we discuss histological approaches for an optimal visualization and quantification of T. cruzi nests in the heart, the main target organ of the parasite. These analyses are important to evaluate the course of the infection in different experimental models and also can be used to investigate parasite colonization and inflammatory processes in other infected tissues and biopsies.
authors.affiliation: Laboratory of Cellular Biology, Department of Biology, ICB, Federal University of Juiz de Fora, Juiz de Fora, MG, Brazil. danifabrino@ufjf.edu.br
authors.forename: Daniela L
authors.initials: DL
authors.lastname: Fabrino
authors.forename: Grazielle A
authors.initials: GA
authors.lastname: Ribeiro
authors.forename: Lívia
authors.initials: L
authors.lastname: Teixeira
authors.forename: Rossana C N
authors.initials: RC
authors.lastname: Melo
mesh_terms: D000818:Animals; D004801:Eosine Yellowish-(YS); D006321:Heart / Q000469:parasitology*; D006416:Hematoxylin; D006652:Histological Techniques / Q000379:methods; D008689:Methacrylates; D008867:Microtomy / Q000379:methods; D051381:Rats; D012394:Rosaniline Dyes; D016610:Tissue Embedding / Q000379:methods; D016707:Tissue Fixation / Q000379:methods; D014048:Tolonium Chloride; D014349:Trypanosoma cruzi / Q000302:isolation & purification* / Q000648:ultrastructure*
chemical_list: D008689:Methacrylates; D012394:Rosaniline Dyes; D014048:Tolonium Chloride; C025485:basic fuchsin; C005044:hydroxyethyl methacrylate; D004801:Eosine Yellowish-(YS); D006416:Hematoxylin