title: ELISA on saliva samples for the detection of anti-hydatid cyst antibodies.
abstract: BACKGROUND
  The detection of antibodies in saliva samples proved to be effective in the diagnosis of several microbial diseases. These antibodies were screened in saliva samples of patients with hydatid cysts.
  
  
  METHODS
  Anti-hydatid fluid antigen IgG and IgA antibodies were screened in saliva and sera of patients with hydatid cysts (n=37) as well as in healthy controls (n=30) using an in-house developed immunoenzymatic assay.
  
  
  RESULTS
  Salivary anti-hydatid fluid antigen IgG showed a sensitivity of 86.5% and a specificity of 80%. A positive correlation was observed between anti-hydatid fluid antigen IgG in saliva and in serum (r = 0.364; p = 0.02).
  
  
  CONCLUSIONS
  The detection of anti-hydatid fluid antigen IgG antibodies in saliva using ELISA promises to be interesting for the diagnosis of cystic echinococcosis.
authors.affiliation: LR 05SP03 Parasitoses émergentes, Institut Pasteur de Tunis, Institut Pasteur de Tunis, Tunisia.
authors.forename: Meriem
authors.initials: M
authors.lastname: Benabid
authors.forename: Yousr
authors.initials: Y
authors.lastname: Galai
authors.forename: Ramzi
authors.initials: R
authors.lastname: Nouira
authors.forename: Nissaf
authors.initials: N
authors.lastname: Ben Alaya
authors.forename: Aida
authors.initials: A
authors.lastname: Bouratbine
authors.forename: Karim
authors.initials: K
authors.lastname: Aoun
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000909:Antibodies, Helminth / Q000032:analysis / Q000097:blood / Q000302:isolation & purification*; D019540:Area Under Curve; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D004444:Echinococcosis, Hepatic / Q000175:diagnosis* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis / Q000097:blood / Q000302:isolation & purification; D007074:Immunoglobulin G / Q000032:analysis / Q000097:blood / Q000302:isolation & purification; D008297:Male; D008875:Middle Aged; D012372:ROC Curve; D012463:Saliva / Q000737:chemistry*
chemical_list: D000909:Antibodies, Helminth; D007070:Immunoglobulin A; D007074:Immunoglobulin G