title: Detection of human adenovirus hexon antigen using carbon nanotube sensors.
abstract: Human adenoviruses (HAdVs) have been implicated in a wide range of diseases affecting primarily the respiratory, ocular and gastrointestinal systems. A rapid and efficient method for the detection of HAdV hexon antigen is described using carbon nanotube (CNT) sensors. Anti-HAdV antibody was immobilised on the reverse surface of a CNT sensor. As a control, non-specific mouse IgG was immobilised on another CNT sensor. I-V(gate) curves were measured after incubation of various concentrations of recombinant HAdVs hexon antigen with anti-HAdVs antibody-immobilised or non-specific mouse IgG-immobilised sensors. The curves showed a positive shift that was dependent on the hexon antigen concentration in the anti-HAdV antibody-immobilised sensor, whereas no such shift was observed in the non-specific mouse IgG-immobilised sensor. The sensitivity of the CNT sensor method was greater than that of enzyme-linked immunosorbent assay. Hence, this method offers a new tool for HAdV detection by analysing antigen-antibody interactions.
authors.affiliation: Department of Ophthalmology, Hokkaido University Graduate School of Medicine, Kita 15, Nishi 7, Kita-ku, Sapporo 060-8638, Japan. sapporo031005@yahoo.co.jp
authors.forename: Xue-Hai
authors.initials: XH
authors.lastname: Jin
authors.forename: Atsushi
authors.initials: A
authors.lastname: Ishii
authors.forename: Koki
authors.initials: K
authors.lastname: Aoki
authors.forename: Susumu
authors.initials: S
authors.lastname: Ishida
authors.forename: Koichi
authors.initials: K
authors.lastname: Mukasa
authors.forename: Shigeaki
authors.initials: S
authors.lastname: Ohno
mesh_terms: D000260:Adenoviruses, Human / Q000302:isolation & purification*; D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D015374:Biosensing Techniques / Q000379:methods; D036022:Capsid Proteins / Q000032:analysis*; D006801:Humans; D051379:Mice; D037742:Nanotubes, Carbon; D012680:Sensitivity and Specificity; D014773:Virology / Q000379:methods*
chemical_list: D000956:Antigens, Viral; D036022:Capsid Proteins; D037742:Nanotubes, Carbon; C018315:hexon capsid protein, Adenovirus