title: Isolation and expression of two distinct Sox8 genes in mudloach (Misgurnus anguillicaudatus).
abstract: To investigate the function and evolutionary origin of the SOXE subgroup, we amplified the genomic DNA of Misgurnus anguillicaudatus using a pair of degenerate primers. Using RACE, we obtained two versions of Sox8 (MaSox8a and MaSox8b) from M. anguillicaudatus. The overall sequence identity of the deduced amino acids from the two genes was 54.38%, with only one amino acid change in the high-mobility group domain. Southern blotting and evidence from the phylogenetic tree provided further proof for the existence of two Sox8 genes at the genomic level. This is the first evidence of two distinct Sox8 genes in Cypriniformes. Semi-quantitative and real-time quantitative PCR assays showed the expression trend of the genes was opposite in early embryonic development, and both were expressed ubiquitously in several adult tissues. The similar expression patterns indicated that MaSox8a and MaSox8b have possible overlapping functions.
authors.affiliation: Molecular and Genetic Laboratory, College of Life Science, Henan Normal University, 46 East Construction Road, Xinxiang, Henan, China. xxhlpf@163.com
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Xia
authors.forename: Jie
authors.initials: J
authors.lastname: Zhao
authors.forename: Qiyan
authors.initials: Q
authors.lastname: Du
authors.forename: Zhongjie
authors.initials: Z
authors.lastname: Chang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism; D003531:Cypriniformes / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D009206:Myocardium / Q000378:metabolism; D009928:Organ Specificity; D010053:Ovary / Q000378:metabolism; D010802:Phylogeny; D055757:SOXE Transcription Factors / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013737:Testis / Q000378:metabolism
chemical_list: D055757:SOXE Transcription Factors