title: Three-wavelength murine photoplethysmography for estimation of vascular gold nanorod concentration.
abstract: Nanoparticle-assisted photo-thermal (NAPT) ablation has become a new and attractive modality for the treatment of cancerous tumors. This therapy exploits the passive accumulation of intravenously delivered optically resonant metal nanoparticles into tumors, however, the circulating bioavailability of these particles is often unknown. We present a non-invasive optical device capable of monitoring the circulation of optically resonant gold nanorods. The device, referred to as a pulse photometer, uses the technique of multi-wavelength photoplethysmography. We simultaneously report the circulation of gold nanorods and oximetry for six hours post-injection in mice with no anesthesia and remove the probe when not collecting data. The instrument shows good agreement (R(2) = 0.903, n = 30) with ex vivo spectrophotometric analysis of blood samples. The real-time feedback provided has a strong potential for reducing variability and thus improving the efficacy of similar clinical therapies.
authors.affiliation: Biomedical Engineering Department, Louisiana Tech University, 818 Nelson Avenue, Ruston, Louisiana 71272, USA.
authors.forename: Gregory J
authors.initials: GJ
authors.lastname: Michalak
authors.forename: Jon A
authors.initials: JA
authors.lastname: Schwartz
authors.forename: Glenn P
authors.initials: GP
authors.lastname: Goodrich
authors.forename: D Patrick
authors.initials: DP
authors.lastname: O'Neal
mesh_terms: D000818:Animals; D001158:Arteries / Q000502:physiology*; D001774:Blood Chemical Analysis / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005260:Female; D006046:Gold / Q000097:blood*; D051379:Mice; D008807:Mice, Inbred BALB C; D043942:Nanotubes / Q000032:analysis*; D010092:Oximetry / Q000295:instrumentation*; D010100:Oxygen / Q000097:blood*; D017156:Photoplethysmography / Q000295:instrumentation*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D006046:Gold; D010100:Oxygen