title: Comparison of array comparative genomic hybridization (aCGH) to FISH and cytogenetics in prognostic evaluation of chronic lymphocytic leukemia.
abstract: BACKGROUND
  High-resolution array comparative genomic hybridization (aCGH) is a method of evaluating chromosomal alterations over the entire genome. We compared aCGH with routine cytogenetics and FISH in detecting genetic alterations in chronic lymphocytic leukemia (CLL).
  
  
  METHODS
  Array comparative genomic hybridization testing was performed on 55 cases of CLL in addition to a standard panel of FISH probes (ATM on 11q22, trisomy 12, 13q14, p53 on 17p13). The frequency of detecting abnormalities was compared, and discordant results between methodologies were compared.
  
  
  RESULTS
  Fifty-five CLL cases [male to female ratio of 2.2:1 and a mean age of 71 (52-90)] were analyzed by both aCGH and FISH. This group of CLL cases showed genetic abnormalities by FISH (60%; 27/45). In contrast to FISH, aCGH detected genetic abnormalities in 82% (45/55) of CLL cases; aCGH identified genetic abnormalities not detected by FISH studies in 16% (7/45) of cases, whereas FISH identified abnormalities not detected by aCGH in only 7% (3/45) of cases. Rare recurring genetic alterations were detected by aCGH including losses in 6q, 8p, 10q, 14q32, and 18q and gains in 10q.
  
  
  CONCLUSIONS
  Our findings suggest aCGH is an effective technique for evaluating recurring genetic abnormalities in CLL and improves on standard FISH in detecting genetic abnormalities in CLL.
authors.affiliation: Clarient, Inc., Columbia, Aliso Viejo, CA 92656-1460, USA. domalley@clarientinc.com
authors.forename: D P
authors.initials: DP
authors.lastname: O'Malley
authors.forename: C
authors.initials: C
authors.lastname: Giudice
authors.forename: A S
authors.initials: AS
authors.lastname: Chang
authors.forename: D
authors.initials: D
authors.lastname: Chang
authors.forename: T S
authors.initials: TS
authors.lastname: Barry
authors.forename: M K
authors.initials: MK
authors.lastname: Hibbard
authors.forename: R
authors.initials: R
authors.lastname: Chen
authors.forename: S-T
authors.initials: ST
authors.lastname: Chen
mesh_terms: D002869:Chromosome Aberrations; D055028:Comparative Genomic Hybridization; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000175:diagnosis* / Q000235:genetics; D008297:Male; D011379:Prognosis