title: Liprin-α1 regulates breast cancer cell invasion by affecting cell motility, invadopodia and extracellular matrix degradation.
abstract: Migration of cells and degradation of the extracellular matrix (ECM) are required for efficient tumor cell invasion, but the underlying molecular mechanisms are only partially known. The PPFIA1 gene for liprin-α1 is frequently amplified in human breast cancers. We recently demonstrated that liprin-α1 is an important regulator of cell edge dynamics during motility. We show, herein, that the liprin-α1 protein is highly expressed in human breast tumors. Functional analysis shows that liprin-α1 is specifically required for the migration and invasion of highly invasive human breast cancer MDA-MB-231 cells. We used time-lapse analysis to demonstrate defects in the motility of liprin-α1-depleted cells that include a striking instability of the lamellipodia. Liprin-α1 levels altered by either RNA interference or overexpression affected also cell spreading and the number of invadopodia per cell, but not the density of invadopodia per unit of surface area. On the other hand, silencing of liprin-α1 inhibited the degradation of the ECM, whereas its overexpression enhanced degradation, resulting in significant negative or positive effects, respectively, on the area of degradation per invadopodium. Transfection of fluorescent-labeled cortactin revealed that depletion of liprin-α1 also affected the assembly and disassembly of invadopodia, with decrease of their lifetime. Our results strongly support a novel important role of liprin-α1 in the regulation of human tumor cell invasion.
authors.affiliation: Cell Adhesion Unit, Division of Neurosciences, San Raffaele Scientific Institute and San Raffaele University, Milano, Italy.
authors.forename: V
authors.initials: V
authors.lastname: Astro
authors.forename: C
authors.initials: C
authors.lastname: Asperti
authors.forename: M G
authors.initials: MG
authors.lastname: Cangi
authors.forename: G
authors.initials: G
authors.lastname: Cangi
authors.forename: C
authors.initials: C
authors.lastname: Doglioni
authors.forename: I
authors.initials: I
authors.lastname: de Curtis
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000502:physiology*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology*; D005109:Extracellular Matrix / Q000378:metabolism*; D005260:Female; D006801:Humans
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C094398:PPFIA1 protein, human