title: Cationized albumin-biocoatings for the immobilization of lipid vesicles.
abstract: Tethered lipid membranes or immobilized lipid vesicles are frequently used as biomimetic systems. In this article, the authors presented a suitable method for efficient immobilization of lipid vesicles onto a broad range of surfaces, enabling analysis by quantitative methods even under rigid, mechanical conditions-bare surfaces such as hydrophilic glass surfaces as well as hydrophobic polymer slides or metal surfaces such as gold. The immobilization of vesicles was based on the electrostatic interaction of zwitterionic or negatively charged lipid vesicles with two types of cationic chemically modified bovine serum albumin (cBSA) blood plasma proteins (cBSA-113 and cBSA-147). Quantitative analysis of protein adsorption was performed as the cBSA coatings were characterized by atomic force microscopy, surface zeta potential measurement, fluorescence microscopy, and surface plasmon spectroscopy, revealing a maximal surface coverage 270-280 ng/cm(2) for 0.02 mg/ml cBSA on gold. Small unilamellar vesicles as well as giant unilamellar vesicles (GUVs) were readily immobilized (∼15 min) on cBSA coated surfaces. GUVs with 5-10 mol% negatively charged 1,2,-dipalmitoyl-sn-glycero-3-phosphoglycerol remained stable in liquid for at least 5 weeks.
authors.affiliation: Max Planck Institute for Polymer Research, Ackermannweg 10, D-55128 Mainz, Germany.
authors.forename: Sandra
authors.initials: S
authors.lastname: Ritz
authors.forename: Klaus
authors.initials: K
authors.lastname: Eisele
authors.forename: Jan
authors.initials: J
authors.lastname: Dorn
authors.forename: Shaohua
authors.initials: S
authors.lastname: Ding
authors.forename: Doris
authors.initials: D
authors.lastname: Vollmer
authors.forename: Sabine
authors.initials: S
authors.lastname: Pütz
authors.forename: Tanja
authors.initials: T
authors.lastname: Weil
authors.forename: Eva-Kathrin
authors.initials: EK
authors.lastname: Sinner
mesh_terms: D000327:Adsorption; D002412:Cations / Q000737:chemistry / Q000378:metabolism*; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000378:metabolism*; D008081:Liposomes / Q000378:metabolism*; D018625:Microscopy, Atomic Force; D011485:Protein Binding; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism*; D013499:Surface Properties
chemical_list: D002412:Cations; D020099:Coated Materials, Biocompatible; D008081:Liposomes; D012710:Serum Albumin, Bovine