title: [A cell model of ischemia/reperfusion injury on skeletal muscle].
abstract: OBJECTIVE
  To establish a model of ischemia/reperfusion injury on L-6TG cell.
  
  
  METHODS
  Cultured L-6TG cells were divided into 2 groups: control group (C), ischemia/reperfusion group (I/R), LDH in culture fluid, SOD, XOD, free calcium in L-6TG cell and mitochondria respiration were evaluated in each group, the micromorphologic changes were observed with microscope.
  
  
  RESULTS
  Compared with control group, after L-6TG cell suffered ischemia 4 hours and reperfusion 4 hours, LDH in culture fluid, XOD, free calcium in L-6TG cell all increased significantly, while SOD in L-6TG cell and mitochondrial respiration decreased, structural damage to L-6TG cell was severe.
  
  
  CONCLUSIONS
  Using mimicking ischemic solution and mimicking reperfusion solution can successfully establish a model of ischemia/reperfusion injury on L-6TG cell.
authors.affiliation: Department of Pathophysiology of North China Coal Medical College, Tangshan 063000, China.
authors.forename: Hong-Jie
authors.initials: HJ
authors.lastname: Li
authors.forename: Guang-Ling
authors.initials: GL
authors.lastname: Zhang
authors.forename: Lian-Yuan
authors.initials: LY
authors.lastname: Zhang
authors.forename: Shu-Yun
authors.initials: SY
authors.lastname: Dong
authors.forename: Xiu-Li
authors.initials: XL
authors.lastname: Men
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003124:Colorimetry; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D018482:Muscle, Skeletal / Q000098:blood supply*; D051381:Rats; D015427:Reperfusion Injury
chemical_list: D007770:L-Lactate Dehydrogenase