title: Immunofluorescence localisation of Banana bunchy top virus (family Nanoviridae) within the aphid vector, Pentalonia nigronervosa, suggests a virus tropism distinct from aphid-transmitted luteoviruses.
abstract: We have applied immunocapture PCR and developed an immunofluorescence assay to specifically detect Banana bunchy top virus (BBTV; family Nanoviridae, genus Babuvirus) within its aphid vector, Pentalonia nigronervosa (Hemiptera, Aphididae). BBTV was localised using either monoclonal or polyclonal antibodies into the anterior midgut (stomach) and into specific cells forming the principal salivary glands. These results suggest a distinct path of virus translocation that likely differs from the one described for aphid-transmitted luteovirus, which enter hemocoels through the hindguts and posterior midguts and that penetrate the accessory salivary glands of their competent vectors. To our understanding, this is the first work analysing the localisation of a virus member of the family Nanoviridae within an aphid vector.
authors.affiliation: Department of Plant and Environmental Protection Sciences, University of Hawaii, 3050 Maile Way, Gilmore Hall 96822 Honolulu, HI, USA. bressan@hawaii.edu
authors.forename: Alberto
authors.initials: A
authors.lastname: Bressan
authors.forename: Shizu
authors.initials: S
authors.lastname: Watanabe
mesh_terms: D000818:Animals; D001042:Aphids / Q000821:virology*; D052658:Babuvirus / Q000502:physiology*; D004279:DNA, Viral; D005455:Fluorescent Antibody Technique; D017793:Luteovirus / Q000502:physiology*; D012469:Salivary Glands / Q000821:virology; D013270:Stomach / Q000821:virology
chemical_list: D004279:DNA, Viral