title: Adeno-associated virus vectors serotype 2 induce prolonged proliferation of capsid-specific CD8+ T cells in mice.
abstract: Using adoptive transfer models we determined that an adeno-associated viral vector of serotype 2 (AAV2) induces in mice proliferation of CD8(+) T cells that recognize an epitope within the viral capsid. Proliferation to an endogenous epitope within viral protein (VP)3 could be observed for at least 3 weeks while a foreign epitope placed at multiple copies within VP2 elicited CD8(+) T cell expansion for at least 10 weeks. These data show that capsid antigens of AAV2 degrade slowly over a period of weeks and during this period provide targets to CD8(+) T cells.
authors.affiliation: Immunology Program, Wistar Institute, Philadelphia, Pennsylvania 19104, USA.
authors.forename: Hua
authors.initials: H
authors.lastname: Li
authors.forename: Steven
authors.initials: S
authors.lastname: Tuyishime
authors.forename: Te-Lang
authors.initials: TL
authors.lastname: Wu
authors.forename: Wynetta
authors.initials: W
authors.lastname: Giles-Davis
authors.forename: Dongming
authors.initials: D
authors.lastname: Zhou
authors.forename: Weidong
authors.initials: W
authors.lastname: Xiao
authors.forename: Katherine A
authors.initials: KA
authors.lastname: High
authors.forename: Hildegund C J
authors.initials: HC
authors.lastname: Ertl
mesh_terms: D000818:Animals; D017951:Antigen Presentation / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D036022:Capsid Proteins / Q000276:immunology*; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology; D000229:Dependovirus / Q000235:genetics*; D018984:Epitopes, T-Lymphocyte / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D005164:Factor IX / Q000235:genetics / Q000378:metabolism; D005260:Female; D023061:Gene Order; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000235:genetics*; D022781:Hepatocytes / Q000276:immunology; D006801:Humans; D007111:Immunity, Cellular; D008213:Lymphocyte Activation / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL
chemical_list: D036022:Capsid Proteins; D018984:Epitopes, T-Lymphocyte; D005164:Factor IX