title: Identification of Pin1-binding phosphorylated proteins in the mouse brain.
abstract: To determine the role of Pin1 in the neurotransmission pathway, Pin1-binding proteins in mouse brain extract were identified. The Pin1-binding proteins were extracted from mouse brain homogenate, and the trypsin-digested peptides were analyzed by nano-liquid chromatography tandem mass spectrometry (LC-MS/MS). Proteins that involve the neurotransmission pathway, such as synapsin I, synapsin II, and calcium/calmodulin-dependent protein kinase type II (CaMKII), were identified in a Mascot search. Pull-down and immunoprecipitation assay indicated that Pin1 binds CaMKII in a phosphorylation-specific manner. It was assumed that Pin1 participates in the neurotransmission pathway involving the phosphorylation signal by CaMKII.
authors.affiliation: Laboratory of Enzymology, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi, Japan.
authors.forename: Yota
authors.initials: Y
authors.lastname: Tatara
authors.forename: Takahiro
authors.initials: T
authors.lastname: Terakawa
authors.forename: Takafumi
authors.initials: T
authors.lastname: Uchida
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D002853:Chromatography, Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D047468:Immunoprecipitation; D008297:Male; D051379:Mice; D000072340:NIMA-Interacting Peptidylprolyl Isomerase; D019696:Peptidylprolyl Isomerase / Q000378:metabolism*; D010750:Phosphoproteins / Q000302:isolation & purification / Q000378:metabolism*; D010766:Phosphorylation; D011485:Protein Binding; D053719:Tandem Mass Spectrometry
chemical_list: D000072340:NIMA-Interacting Peptidylprolyl Isomerase; D010750:Phosphoproteins; D019696:Peptidylprolyl Isomerase; C000606850:Pin1 protein, mouse