title: [Heart-type fatty acid-binding protein alteration in rat's cardiocyte after acute myocardial infarction].
abstract: OBJECTIVE
  To explore the changes of mRNA and protein expressions of heart-type fatty acid binding protein (H-FABP) in rat ischemic myocardium at different intervals ischemia.
  
  
  METHODS
  60 SD male rats weighing 250-350 g, were randomly divided into one sham-operated group and five study groups (group A1, A2, A3, A4, A5, the left coronary artery of rats has been ligated for 1 h, 2 h, 4 h, 6 h, 12 h respectively). Myocardil samples from infarct zone, ischemic and non-ischemic zone, were obtained for histology examination, and the mRNA for H-FABP in ischemic myocardial tissue were determined by RT-PCR. Serum free fatty acid(FFA) was determined by colorimetric method.
  
  
  RESULTS
  Compared to sham hearts, H-FABP mRNA expression were significantly decreased in ischemia zone of AMI rat hearts (P < 0.05), especially in rats underwent 4 h ischemia and 6 h ischemia (P < 0.01). Serum FFA were significantly increased in AMI rats relative to sham rats (P < 0.05).
  
  
  CONCLUSIONS
  Significant down-regulated heart-type fatty acid binding protein after myocardial ischemia might play an important role in myocardial injury and energy metabolism disorder.
authors.affiliation: Henan Province Jiyuan People's Hospital, Jiyuan 454650, China.
authors.forename: Hong-yan
authors.initials: HY
authors.lastname: Wang
authors.forename: Xiao-yun
authors.initials: XY
authors.lastname: Zhao
authors.forename: Fa-zhong
authors.initials: FZ
authors.lastname: Yang
authors.forename: Han-ying
authors.initials: HY
authors.lastname: Xing
authors.forename: Xue
authors.initials: X
authors.lastname: Guo
mesh_terms: D000818:Animals; D015536:Down-Regulation; D050556:Fatty Acid-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D008297:Male; D009203:Myocardial Infarction / Q000378:metabolism*; D032383:Myocytes, Cardiac / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D050556:Fatty Acid-Binding Proteins; D012333:RNA, Messenger