title: Rapid determination of fumonisins in corn-based products by liquid chromatography/tandem mass spectrometry.
abstract: A simple, fast, and robust method was developed for the determination of fumonisin B1 (FB1), fumonisin B2 (FB2), and fumonisin B3 (FB3) in corn-based human food and animal feed (cornmeal). The method involves a single extraction step followed by centrifugation and filtration before analysis by ultra-performance liquid chromatographylelectrospray ionization (UPLC/ESI)-MS/MS. The LC/MS/MS method developed here represents the fastest and simplest procedure (<30 min) among both conventional HPLC methods and other LC/MS methods using SPE cleanup. The potential for high throughput analysis makes the method particularly beneficial for regulatory agencies and analytical laboratories with a high sample volume. A single-laboratory validation was conducted by testing three different spiking levels (200, 500, and 1000 ng/g for FB1 and FB2; 100, 250, and 500 ng/g for FB3) for accuracy and precision. Recoveries of FB1 ranged from 93 to 98% with RSD values of 3-8%. Recoveries of FB2 ranged from 104 to 108%, with RSD values of 2-6%. Recoveries of FB3 ranged from 94 to 108%, with RSD values of 2-5%.
authors.affiliation: Texas A&M University, Texas AgriLife Research, Office of the Texas State Chemist, 445 Agronomy Rd, College Station, TX 77843, USA.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Timothy J
authors.initials: TJ
authors.lastname: Herrman
authors.forename: Susie Y
authors.initials: SY
authors.lastname: Dai
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D037341:Fumonisins / Q000032:analysis*; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*; D003313:Zea mays / Q000737:chemistry*
chemical_list: D037341:Fumonisins