title: Angular domain fluorescence lifetime imaging: a tissue-like phantom study.
abstract: We describe a fluorescence lifetime imaging technique employing the collimation detection capabilities of an angular filter array (AFA). The AFA accepts minimally scattered photons emitted from fluorophores up to 2 mm deep within turbid media. The technique, referred to as Angular Domain Fluorescence Lifetime Imaging (ADFLI), is described and its performance evaluated in comparison to a conventional (lens and pinhole) system. Results from a tissue-mimicking phantom demonstrated that ADFLI provides better spatial resolution and image contrast for fluorescent probes at greater depths compared to a lens and pinhole system.
authors.affiliation: Imaging Program, Lawson Health Research Institute, London, ON, Canada.
authors.forename: Mohamadreza
authors.initials: M
authors.lastname: Najiminaini
authors.forename: Fartash
authors.initials: F
authors.lastname: Vasefi
authors.forename: Kenneth M
authors.initials: KM
authors.lastname: Tichauer
authors.forename: Ting-Yim
authors.initials: TY
authors.lastname: Lee
authors.forename: Bozena
authors.initials: B
authors.lastname: Kaminska
authors.forename: Jeffrey J L
authors.initials: JJ
authors.lastname: Carson
mesh_terms: D005453:Fluorescence; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008855:Microscopy, Electron, Scanning; D019047:Phantoms, Imaging; D013997:Time Factors