title: [Effect of nitric oxide on the somatic membrane of rat DRG neurons].
abstract: OBJECTIVE
  To observe the role of nitric oxide in dorsal root ganglion (DRG) neurons and its related ionic mechanisms, and explore the function of NO in pain transmission process.
  
  
  METHODS
  In freshly isolated rat DRG samples, using intracellular recording technique, we perfused sodium nitroprusside (NO donor) to observe the role of NO in DRG neurons.
  
  
  RESULTS
  In 77.45% of the bath cells, application of sodium nitroprusside (10 -100 mmol/L) induced concentration-dependent membrane hyperpolarization (79/102), and remaining neurons had no response. The membrane conductance increased from control value of (21.06 +/- 1.94) nS to (23.08 +/- 0.92) nS during sodium nitroprusside induced hyperpolarization. L-NAME (1 mmol/L), CdCl2 (0.1 mmol/L) and non-sodium BSS failed to change the amplitude of sodium nitroprusside induced hyperpolarization. When BSS containing 10 mmol/L TEA was used, sodium nitroprusside induced hyperpolarization was obviously inhibited.
  
  
  CONCLUSIONS
  Sodium nitroprusside could cause concentration-dependent hyperpolarization in DRG neurons by activating K+ channels.
authors.affiliation: Division of Electrophysiology, Key Laboratory of Xinjiang Endemic and Ethnic Diseases, Ministry of Education, School of Medical, Shihezi University, Shihezi 832002, China.
authors.forename: Hong-Ju
authors.initials: HJ
authors.lastname: Cheng
authors.forename: Ke-Tao
authors.initials: KT
authors.lastname: Ma
authors.forename: Lei
authors.initials: L
authors.lastname: Zhao
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: Ying-Ying
authors.initials: YY
authors.lastname: Cao
authors.forename: Jun-Qiang
authors.initials: JQ
authors.lastname: Si
mesh_terms: D000818:Animals; D005260:Female; D005727:Ganglia, Spinal / Q000502:physiology*; D008297:Male; D008564:Membrane Potentials / Q000502:physiology*; D009474:Neurons / Q000502:physiology; D009569:Nitric Oxide / Q000494:pharmacology*; D009599:Nitroprusside / Q000494:pharmacology; D010146:Pain / Q000503:physiopathology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D009599:Nitroprusside; D009569:Nitric Oxide