title: Simultaneous determination of ramipril, ramiprilat and telmisartan in human plasma using liquid chromatography tandem mass spectrometry.
abstract: A rapid and sensitive liquid chromatography tandem mass spectrometry method has been developed and validated for the simultaneous determination of ramipril, ramiprilat and telmisartan in human plasma. The solid-phase extraction technique was used for the extraction of ramipril, ramiprilat and telmisartan from human plasma. Trandolaprilat and hydrochlorothiazide were used as the internal standards (ISs). Chromatography was performed on a Hypurity C18, 5 μm, 50 mm × 4.6mm column, with the mobile phase consisting of ammonium acetate and acetonitrile (in a 20:80 ratio), followed by detection using mass spectrometry. The method involves a simple reversed isocratic chromatography condition and mass spectrometry detection, which enables detection at sub-nanogram levels. The method was validated and the lower limit of quantification for ramipril, ramiprilat and telmisartan was found to be 0.1 ng mL(-1), 0.1 ng mL(-1) and 2 ng mL(-1), respectively. The mean recovery for ramipril, ramiprilat and telmisartan ranged from 90.1 to 104.1%. This method increased the sensitivity and selectivity; resulting in high-throughput analysis of ramipril, ramiprilat and telmisartan using two different ISs in a single experiment for bioequivalence studies, with a chromatographic run time of 1.5 min only.
authors.affiliation: Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee UA 247 667, India. vinodfcy@gmail.com
authors.forename: V K
authors.initials: VK
authors.lastname: Gupta
authors.forename: Rajeev
authors.initials: R
authors.lastname: Jain
authors.forename: Ojitkumar
authors.initials: O
authors.lastname: Lukram
authors.forename: Shilpi
authors.initials: S
authors.lastname: Agarwal
authors.forename: Ashish
authors.initials: A
authors.lastname: Dwivedi
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors / Q000097:blood*; D001562:Benzimidazoles / Q000097:blood; D001565:Benzoates / Q000097:blood; D001774:Blood Chemical Analysis / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D017257:Ramipril / Q000031:analogs & derivatives / Q000097:blood; D053719:Tandem Mass Spectrometry / Q000379:methods*; D000077333:Telmisartan; D013997:Time Factors
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D001562:Benzimidazoles; D001565:Benzoates; C052549:ramiprilat; D017257:Ramipril; D000077333:Telmisartan