title: The glypican 3-hosted murine mir717 gene: sequence conservation, seed region polymorphisms and putative targets.
abstract: Mir717 (mmu-mir-717) was first reported in mouse and resides in the intron 3 of glypican 3 (Gpc3) gene. Our present study revealed that this microRNA (miRNA) gene is conserved among 26 mammalian species and harbors polymorphic sites within the mature seed region in mice. Our finding represents a rare four layer genomic overlap consisting of growth associated quantitative trait locus (QTL), body mass associated Gpc3 gene, highly conserved miRNA gene and mature miRNA seed single nucleotide polymorphism (SNP) identified in the lean mouse strain 129/Sv. Additionally, genes potentially targeted by Mir717 include 91 genes associated with obesity and related phenotypes in mammals. Our analysis provides a basis for further experiments to causally connect the identified SNP and Mir717 gene itself to obesity regulation. Furthermore, our bioinformatics analysis now enables functional annotation of Mir717 orthologs in other species, thus determining the effect of its target genes on fat-related traits.
authors.affiliation: Department of Animal Science, Biotechnical Faculty, University of Ljubljana, Groblje 3, SI- 1230 Domzale, Slovenia. jiangz@wsu.edu
authors.forename: Tanja
authors.initials: T
authors.lastname: Kunej
authors.forename: Dasa Jevsinek
authors.initials: DJ
authors.lastname: Skok
authors.forename: Simon
authors.initials: S
authors.lastname: Horvat
authors.forename: Peter
authors.initials: P
authors.lastname: Dovc
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Jiang
mesh_terms: D000818:Animals; D001483:Base Sequence; D019295:Computational Biology; D053673:Glypicans / Q000235:genetics*; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D040641:Quantitative Trait Loci / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
chemical_list: D053673:Glypicans; D035683:MicroRNAs