title: Chinese prescription Kangen-karyu prevents dyslipidaemia and oxidative stress in mouse model of type 2 diabetes.
abstract: OBJECTIVE
  We have investigated the effects of Kangen-karyu, a Chinese prescription, on the lipid metabolism and oxidative stress in a type 2 diabetes model.
  
  
  METHODS
  Male db/db mice were divided into three groups: control (vehicle), Kangen-karyu 100 or 200 mg/kg body weight/day orally administered mice. Age-matched non-diabetic m/m mice were used as a normal group.
  
  
  RESULTS
  The administration of Kangen-karyu reduced hyperglycaemia and hyperlipidaemia in db/db type 2 diabetic mice through a decline in the serum levels of glucose and lipids, and an improvement of lipoprotein profiles. The increased oxidative stress in db/db mice was attenuated by the administration of Kangen-karyu through inhibiting the generation of reactive oxygen species and lipid peroxidation. The enhanced hepatic triglyceride and total cholesterol levels of the db/db mice were significantly reduced by Kangen-karyu administration through down-regulation of sterol regulatory element-binding protein-1 and lipogenic enzymes in liver. Furthermore, the expressions of hepatic nuclear factor-kappa B (NF-κB) and cyclooxygenase-2 and inducible nitric oxide synthase protein levels were also augmented in db/db mice. However, Kangen-karyu reduced the expressions of these inflammatory proteins by inhibiting NF-κB activation in db/db type 2 diabetes.
  
  
  CONCLUSIONS
  This study suggests that Kangen-karyu may improve oxidative stress via the regulation of dyslipidaemia in type 2 diabetes.
authors.affiliation: Institute of Natural Medicine, University of Toyama, Sugitani, Toyama, Japan.
authors.forename: Jeong Sook
authors.initials: JS
authors.lastname: Noh
authors.forename: Chan Hum
authors.initials: CH
authors.lastname: Park
authors.forename: Hyun Young
authors.initials: HY
authors.lastname: Kim
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhao
authors.forename: Noriko
authors.initials: N
authors.lastname: Yamabe
authors.forename: Kinzo
authors.initials: K
authors.lastname: Matsumoto
authors.forename: Takako
authors.initials: T
authors.lastname: Yokozawa
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000187:drug effects; D002784:Cholesterol / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000150:complications / Q000188:drug therapy*; D003924:Diabetes Mellitus, Type 2 / Q000150:complications / Q000188:drug therapy*; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology*; D050171:Dyslipidemias / Q000209:etiology / Q000517:prevention & control*; D050356:Lipid Metabolism / Q000187:drug effects; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D016328:NF-kappa B / Q000187:drug effects / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism; D014280:Triglycerides / Q000378:metabolism
chemical_list: D001786:Blood Glucose; D004365:Drugs, Chinese Herbal; C468270:Kangen-karyu; D016328:NF-kappa B; D017382:Reactive Oxygen Species; D014280:Triglycerides; D002784:Cholesterol