title: [Simultaneous determination of five flavonoid compounds in leaves of Adinandra nitida by HPLC-PAD].
abstract: OBJECTIVE
  To established a novel HPLC-PAD method for the simultaneous determination of five compounds (camellianin A, camellianin B, apigenin, quercitrin, epicatechin) in leaves of Adinandra nitida.
  
  
  METHODS
  These compounds were effectively separated on a reversed-phase C18 column (4.6 mm x 250 mm, 10 microm) with the column temperature at 25 degrees C. The mobile phase was composed of 0.1% aqueous formic acid and methanol. The flow rate was 1.0 mL x min(-1), the detection wavelength was set at 280 nm before 15 min for detecting epicatechin and at 262 nm from 15 to 40 min for detecting the rest four compounds.
  
  
  RESULTS
  All the linearities were good (r > 0.9991) within their test ranges. The established method showed good precision with overall intra-day and interday RSD values less than 3.33% and 3.2%, respectively. The average recoveries were in the range of 96.08% to 100.30% with RSD from 2.0% to 4.0%. The limits of detection (LOD) and quantification (LOQ) in the range of ranged from 0.9 to 5.6 ng and 3.0 to 19.0 ng, respectively.
  
  
  CONCLUSIONS
  This established method can be applied to evaluate the intrinsic quality of the leaves of A. nitida.
authors.affiliation: Institute of Modern Chinese Medicine, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Wensong
authors.initials: W
authors.lastname: Zuo
authors.forename: Juanhu
authors.initials: J
authors.lastname: Xu
authors.forename: Changxin
authors.initials: C
authors.lastname: Zhou
authors.forename: Lishe
authors.initials: L
authors.lastname: Gan
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D005419:Flavonoids / Q000032:analysis*; D019684:Magnoliopsida / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry
chemical_list: D004365:Drugs, Chinese Herbal; D005419:Flavonoids