title: Introduction to fluorescence microscopy.
abstract: This chapter is an overview of basic principles of fluorescence microscopy, including a brief history on the invention of this type of microscopy. The chapter highlights important points related to properties of fluorochromes, resolution in fluorescence microscopy, phase contrast and fluorescence, fluorescence filters, construction of a fluorescence microscope, and tips on the correct use of this equipment.
authors.affiliation: Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA. ighiran@bidmc.harvard.edu
authors.forename: Ionita C
authors.initials: IC
authors.lastname: Ghiran
mesh_terms: D005374:Filtration / Q000379:methods; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D049673:History, 20th Century; D049674:History, 21st Century; D007909:Lenses; D008856:Microscopy, Fluorescence / Q000266:history / Q000295:instrumentation* / Q000379:methods*; D008858:Microscopy, Phase-Contrast / Q000379:methods; D055620:Optical Phenomena
chemical_list: D005456:Fluorescent Dyes