title: Cytochromes P450: exploiting diversity and enabling application as biocatalysts.
abstract: The remarkable chemical reactivity and substrate range displayed by cytochromes P450 (P450s) renders them attractive as potential catalysts for a host of challenging chemical reactions in industry. The opportunities afforded by these biocatalysts are increased by the availability of greater diversity provided by the genomic resource and the variant libraries of well-known P450s produced by rational and random engineering techniques. The exploitation of this enormous diversity will require novel tools in screening, to identify enzyme reactions of interest, and also in the enabling of these valuable activities through protein engineering and bioprocess optimisation.
authors.affiliation: York Structural Biology Laboratory, Department of Chemistry, University of York, York YO10 5YW, UK. grogan@ysbl.york.ac.uk
authors.forename: Gideon
authors.initials: G
authors.lastname: Grogan
mesh_terms: D000818:Animals; D001419:Bacteria / Q000201:enzymology / Q000235:genetics; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics* / Q000378:metabolism; D005658:Fungi / Q000201:enzymology / Q000235:genetics; D023281:Genomics / Q000379:methods; D006801:Humans; D015202:Protein Engineering / Q000379:methods*
chemical_list: D003577:Cytochrome P-450 Enzyme System