title: Improving isothermal DNA amplification speed for the rapid detection of Mycobacterium tuberculosis.
abstract: In this study, we report the development of a helicase-dependent amplification assay for rapid detection of Mycobacterium tuberculosis (MTB). By applying a step-by-step optimization method, the amplification time from an input of 2-copy MTB genomic DNA was reduced from about 60 min to less than 30 min.
authors.affiliation: BioHelix Corporation, Beverly, MA 01915, USA.
authors.forename: Aurélie
authors.initials: A
authors.lastname: Motré
authors.forename: Richard
authors.initials: R
authors.lastname: Kong
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D004265:DNA Helicases / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D013696:Temperature; D013997:Time Factors; D014376:Tuberculosis / Q000175:diagnosis*
chemical_list: D004269:DNA, Bacterial; D004265:DNA Helicases