title: [The effects of repetitive limb ischemia on the systemic concentration of NO, NOS in plasma of healthy humans].
abstract: OBJECTIVE
  To investigate the effects of RLI on plasma nitric oxide (NO) and NO synthase (NOS) isoforms of healthy humans.
  
  
  METHODS
  30 healthy human subjects (aged from 40 - 70 years old) were recruited. RLI was induced by five 5 min cycles of ischemia of non dominant arm (200 mmHg, 5 min interval). Blood pressure, heart rate, and the feelings of ischemic arm were continuously monitored. Venous plasma was collected in contralateral arm at Pre, Post-0 h, Post-4 h, and Post-24 h. Plasma level of NO was measured by Griess reaction, and NOS was measured by chemical method.
  
  
  RESULTS
  Blood pressure and heart rate varied in normal range. The uncomfortable feeling was decreased with the increasing numbers of ischemic cycles. Plasma level of NO, and iNOS in plasma were significantly increased at Post-0 h, Post-4 h, and Post-24 h compared to Pre (P < 0.05). tNOS was also significantly increased at Post-0 h and Post-4 h compared to Pre (P < 0.05). No significant change in plasma cNOS was shown at following three time points than Pre.
  
  
  CONCLUSIONS
  These findings suggest that RLI can elevate plasma level of NO, tNOS, and iNOS in healthy humans. RLI might be a safe method as a rIPC, and it would have important possibility to be performed in clinic.
authors.affiliation: Cerebrovascular Disease Lab, Xuan Wu Hospital, China.
authors.forename: Sha
authors.initials: S
authors.lastname: Dang
authors.forename: Yu-Min
authors.initials: YM
authors.lastname: Luo
authors.forename: Xun-Ming
authors.initials: XM
authors.lastname: Ji
authors.forename: Guowei
authors.initials: G
authors.lastname: Lu
authors.forename: Wei-Zhen
authors.initials: WZ
authors.lastname: Niu
authors.forename: Shu-Ting
authors.initials: ST
authors.lastname: Li
authors.forename: Feng
authors.initials: F
authors.lastname: Ling
mesh_terms: D000328:Adult; D000368:Aged; D001132:Arm / Q000098:blood supply*; D005260:Female; D006801:Humans; D007511:Ischemia / Q000097:blood / Q000503:physiopathology*; D019194:Ischemic Preconditioning / Q000379:methods*; D008297:Male; D008875:Middle Aged; D009569:Nitric Oxide / Q000097:blood*; D019001:Nitric Oxide Synthase / Q000097:blood / Q000378:metabolism*; D015427:Reperfusion Injury / Q000503:physiopathology / Q000517:prevention & control
chemical_list: D009569:Nitric Oxide; D019001:Nitric Oxide Synthase