title: B-type natriuretic peptide pretreatment attenuates heart ischemia-reperfusion injury in rats.
abstract: BACKGROUND
  Graft dysfunction following transplantation is due to ischemia-reperfusion (IR) injury. B-type natriuretic peptide (BNP) has been shown to attenuate ischemia-reperfusion (IR) injury in animals. We designed this experiment to evaluate the effects of BNP pretreatment on IR-induced heart injury.
  
  
  METHODS
  A rat model of myocardial IR injury was established by ligating the left descending coronary artery for 30 minutes before a 2-hour reperfusion. We randomly divided 47 Sprague-Dawley rats into six groups: sham operation (n = 6), IR (n = 9), IR + BNP 0.001 μg/(kg·min; n = 8); IR + BNP 0.005 μg/(kg·min; n = 8); IR + BNP 0.01 μg/(kg·min; n = 8); and IR + BNP 0.03 μg/(kg·min; n = 8). During the processes, lead ECG was traced continuously to note arrhythmias caused by reperfusion. Serum levels of creatine kinase-MB (CK-MB) and lactate dehydrogenase (LDH) were measured at 2 hours after reperfusion.
  
  
  RESULTS
  The BNP-treated animals showed a significantly reduced incidence of ventricular arrhythmias caused by reperfusion (P < .05). They showed a dosage-dependent marked decrease in levels of serum CK-MB and LDH compared with the IR only group (P < .05).
  
  
  CONCLUSIONS
  BNP pretreatment protected the myocardium of rats against IR injury, in a dose-dependent manner.
authors.affiliation: Department of Thoracic Surgery, Cancer Institute and Hospital of Jiangsu Province, Nanjing, Jiangsu, China.
authors.forename: B
authors.initials: B
authors.lastname: Ren
authors.forename: H
authors.initials: H
authors.lastname: Wu
authors.forename: R
authors.initials: R
authors.lastname: Yin
authors.forename: L
authors.initials: L
authors.lastname: Xu
authors.forename: H
authors.initials: H
authors.lastname: Jing
authors.forename: M
authors.initials: M
authors.lastname: Li
authors.forename: F
authors.initials: F
authors.lastname: Jiang
authors.forename: Z
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000818:Animals; D008297:Male; D015428:Myocardial Reperfusion Injury / Q000188:drug therapy*; D020097:Natriuretic Peptide, Brain / Q000008:administration & dosage* / Q000627:therapeutic use; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D020097:Natriuretic Peptide, Brain