title: Enzymatic and molecular characterization of an endo-1,3-β-d-glucanase from the crystalline styles of the mussel Perna viridis.
abstract: The retaining endo-1,3-β-d-glucanase (EC 3.2.1.39) was isolated from the crystalline styles of the commercially available Vietnamese edible mussel Perna viridis. It catalyzes hydrolysis of β-1,3-bonds in glucans and enables to catalyze a transglycosylation reaction. Resources of mass-spectrometry for analysis of enzymatic products were studied. cDNA sequence of endo-1,3-β-d-glucanase was determined by RT-PCR in conjunction with the rapid amplification of cDNA ends (RACE) methods. The cDNA of 1380bp contains an open reading frame of 1332bp encoding a mature protein of 328 amino acids. On basis of amino acid sequence analysis endo-1,3-β-d-glucanase was classified as a glycoside hydrolase of family 16.
authors.affiliation: Pacific Institute of Bioorganic Chemistry FEB RAS, Vladivostok, 690022, pr.100 let Vladivostoku 159, Russia.
authors.forename: Alexander M
authors.initials: AM
authors.lastname: Zakharenko
authors.forename: Mikhail I
authors.initials: MI
authors.lastname: Kusaykin
authors.forename: Svetlana N
authors.initials: SN
authors.lastname: Kovalchuk
authors.forename: Stanislav D
authors.initials: SD
authors.lastname: Anastyuk
authors.forename: Bui Minh
authors.initials: BM
authors.lastname: Ly
authors.forename: Victoria V
authors.initials: VV
authors.lastname: Sova
authors.forename: Valeriy A
authors.initials: VA
authors.lastname: Rasskazov
authors.forename: Tatyana N
authors.initials: TN
authors.lastname: Zvyagintseva
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D018076:DNA, Complementary / Q000235:genetics; D004693:Glucan Endo-1,3-beta-D-Glucosidase / Q000737:chemistry* / Q000145:classification / Q000235:genetics; D008969:Molecular Sequence Data; D009132:Muscles / Q000201:enzymology*; D049883:Perna / Q000201:enzymology* / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D004693:Glucan Endo-1,3-beta-D-Glucosidase