title: [Determination of DNA damage in vitro].
abstract: Ionising irradiation acts primarily via induction of DNA damage, among which double-strand breaks are the most important lesions. These lesions may lead to lethal chromosome aberrations, which are the main reason for cell inactivation. Double-strand breaks can be repaired by several different mechanisms. The regulation of these mechanisms appears be fairly different for normal and tumour cells. Among different cell lines capacity of double-strand break repair varies by only few percents and is known to be determined mostly by genetic factors. Knowledge about double-strand break repair mechanisms and their regulation is important for the optimal application of ionising irradiation in medicine.
authors.affiliation: Lab of Radiobiology & Experimental Radiooncology, University Medical Center Hamburg-Eppendorf, 20246 Hamburg. dikomey@uke.uni-hamburg.de
authors.forename: E
authors.initials: E
authors.lastname: Dikomey
mesh_terms: D000818:Animals; D004247:DNA / Q000235:genetics* / Q000528:radiation effects*; D004249:DNA Damage / Q000502:physiology*; D004252:DNA Mutational Analysis / Q000379:methods*; D006801:Humans; D011829:Radiation Dosage
chemical_list: D004247:DNA