title: UV LED lighting for automated crystal centring.
abstract: A direct outcome of the exponential growth of macromolecular crystallography is the continuously increasing demand for synchrotron beam time, both from academic and industrial users. As more and more projects entail screening a profusion of sample crystals, fully automated procedures at every level of the experiments are being implemented at all synchrotron facilities. One of the major obstacles to achieving such automation lies in the sample recognition and centring in the X-ray beam. The capacity of UV light to specifically react with aromatic residues present in proteins or with DNA base pairs is at the basis of UV-assisted crystal centring. Although very efficient, a well known side effect of illuminating biological samples with strong UV sources is the damage induced on the irradiated samples. In the present study the effectiveness of a softer UV light for crystal centring by taking advantage of low-power light-emitting diode (LED) sources has been investigated. The use of UV LEDs represents a low-cost solution for crystal centring with high specificity.
authors.affiliation: Structural Biology Research Center, Photon Factory, High Energy Research Organization (KEK), 1-1 Oho, Tsukuba, Ibaraki 305-0801, Japan. leonard.chavas@kek.jp
authors.forename: Leonard M G
authors.initials: LM
authors.lastname: Chavas
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Yamada
authors.forename: Masahiko
authors.initials: M
authors.lastname: Hiraki
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Igarashi
authors.forename: Naohiro
authors.initials: N
authors.lastname: Matsugaki
authors.forename: Soichi
authors.initials: S
authors.lastname: Wakatsuki
mesh_terms: D057205:Automation, Laboratory / Q000379:methods; D018360:Crystallography, X-Ray / Q000379:methods*; D011506:Proteins / Q000737:chemistry / Q000528:radiation effects; D014466:Ultraviolet Rays
chemical_list: D011506:Proteins