title: Cadmium chloride induces the expression of metallothionein mRNA by endometrial stromal cells and amnion-derived (WISH) cells.
abstract: BACKGROUND
  Metallothionein (MT) is known to bind to metals with high affinity. The potential for MT-1 mRNA expression in endometrial stromal cells (ESC) and amniotic cells in response to cytokines and cadmium chloride (CdCl(2)) was evaluated.
  
  
  METHODS
  Human ESC were cultured and treated with interleukin-1α, 12-O-tetradecanoylphobol 13-acetate (TPA), forskolin, transforming growth factor-β, and CdCl(2). Amnion-derived (WISH) cells were also cultured and treated with the same reagents. The levels of MT mRNA were evaluated by Northern blot analysis in ESC and WISH cells.
  
  
  RESULTS
  In response to treatment with CdCl(2) (0.01-10 μM), the expression of MT mRNA markedly increased in ESC and WISH cells in a dose-dependent manner. On the other hand, the expression of MT mRNA did not increase after treatment with interleukin-1α (1 nM), TPA (10 nM), forskolin (1 μM) or transforming growth factor-β (1 nM) in these cells.
  
  
  CONCLUSIONS
  These findings indicate that MT expression in ESC and WISH cells is sensitive to the CdCl(2) concentration, which is known to be evaluated in cigarette smokers. The present results suggest that increased levels of MT may affect metal metabolism at the feto-maternal interface.
authors.affiliation: Department of Obstetrics and Gynecology, Faculty of Medicine, Oita University, Yufu, Japan. kawayas @ med.oita-u.ac.jp
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Kawano
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Furukawa
authors.forename: Yukie
authors.initials: Y
authors.lastname: Kawano
authors.forename: Wakana
authors.initials: W
authors.lastname: Abe
authors.forename: Tomoko
authors.initials: T
authors.lastname: Hirakawa
authors.forename: Hisashi
authors.initials: H
authors.lastname: Narahara
mesh_terms: D000650:Amnion / Q000737:chemistry / Q000166:cytology*; D019256:Cadmium Chloride / Q000494:pharmacology*; D002461:Cell Line, Transformed; D002478:Cells, Cultured; D005576:Colforsin / Q000494:pharmacology; D004717:Endometrium / Q000166:cytology*; D005260:Female; D015870:Gene Expression / Q000187:drug effects*; D006801:Humans; D053582:Interleukin-1alpha / Q000494:pharmacology; D008668:Metallothionein / Q000235:genetics*; D011247:Pregnancy; D012333:RNA, Messenger / Q000032:analysis; D012907:Smoking; D017154:Stromal Cells / Q000737:chemistry / Q000378:metabolism*; D016212:Transforming Growth Factor beta / Q000494:pharmacology
chemical_list: D053582:Interleukin-1alpha; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; D005576:Colforsin; D008668:Metallothionein; D019256:Cadmium Chloride