title: Structural characterization and study of immunoenhancing and antioxidant property of a novel polysaccharide isolated from the aqueous extract of a somatic hybrid mushroom of Pleurotus florida and Calocybe indica variety APK2.
abstract: A water-soluble polysaccharide was isolated from the aqueous extract of the fruit bodies of somatic hybrid PCH9FB, obtained through intergeneric protoplast fusion between the strains Pleurotus florida and Calocybe indica var. On the basis of total acid hydrolysis, the polysaccharide was found to contain galactose, fucose, and glucose in a molar ratio of nearly 2:1:2. Methylation analysis and NMR experiments ((1)H, (13)C, DEPT-135, DQF-COSY, TOCSY, NOESY, ROESY, HMQC, and HMBC) showed that the structure of the repeating unit present in the polysaccharide was This molecule showed macrophage, splenocyte, thymocyte activation as well as antioxidant property.
authors.affiliation: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721102, West Bengal, India.
authors.forename: Kousik
authors.initials: K
authors.lastname: Maity
authors.forename: Eshita
authors.initials: E
authors.lastname: Kar Mandal
authors.forename: Saikat
authors.initials: S
authors.lastname: Maity
authors.forename: Sanjoy K
authors.initials: SK
authors.lastname: Gantait
authors.forename: Debsankar
authors.initials: D
authors.lastname: Das
authors.forename: Swatilekha
authors.initials: S
authors.lastname: Maiti
authors.forename: Tapas K
authors.initials: TK
authors.lastname: Maiti
authors.forename: Samir R
authors.initials: SR
authors.lastname: Sikdar
authors.forename: Syed S
authors.initials: SS
authors.lastname: Islam
mesh_terms: D000363:Agaricales / Q000737:chemistry*; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D049109:Cell Proliferation; D006824:Hybridization, Genetic; D008262:Macrophage Activation; D017737:Macrophages, Peritoneal / Q000166:cytology / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism; D020076:Pleurotus / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry* / Q000276:immunology* / Q000302:isolation & purification; D013154:Spleen / Q000166:cytology; D013950:Thymus Gland / Q000166:cytology; D014867:Water / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D011134:Polysaccharides; D014867:Water; D009569:Nitric Oxide