title: TRPM7 regulates gastrulation during vertebrate embryogenesis.
abstract: During gastrulation, cells in the dorsal marginal zone polarize, elongate, align and intercalate to establish the physical body axis of the developing embryo. Here we demonstrate that the bifunctional channel-kinase TRPM7 is specifically required for vertebrate gastrulation. TRPM7 is temporally expressed maternally and throughout development, and is spatially enriched in tissues undergoing convergent extension during gastrulation. Functional studies reveal that TRPM7's ion channel, but not its kinase domain, specifically affects cell polarity and convergent extension movements during gastrulation, independent of mesodermal specification. During gastrulation, the non-canonical Wnt pathway via Dishevelled (Dvl) orchestrates the activities of the GTPases Rho and Rac to control convergent extension movements. We find that TRPM7 functions synergistically with non-canonical Wnt signaling to regulate Rac activity. The phenotype caused by depletion of the Ca(2+)- and Mg(2+)-permeant TRPM7 is suppressed by expression of a dominant negative form of Rac, as well as by Mg(2+) supplementation or by expression of the Mg(2+) transporter SLC41A2. Together, these studies demonstrate an essential role for the ion channel TRPM7 and Mg(2+) in Rac-dependent polarized cell movements during vertebrate gastrulation.
authors.affiliation: Department of Biology, College of Science and Technology, Temple University, Philadelphia, PA 19122, USA.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Li-Ting
authors.initials: LT
authors.lastname: Su
authors.forename: Deepak K
authors.initials: DK
authors.lastname: Khadka
authors.forename: Courtney
authors.initials: C
authors.lastname: Mezzacappa
authors.forename: Yuko
authors.initials: Y
authors.lastname: Komiya
authors.forename: Akira
authors.initials: A
authors.lastname: Sato
authors.forename: Raymond
authors.initials: R
authors.lastname: Habas
authors.forename: Loren W
authors.initials: LW
authors.lastname: Runnels
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000502:physiology; D000818:Animals; D002465:Cell Movement; D000072261:Dishevelled Proteins; D047108:Embryonic Development; D054262:Gastrulation; D008274:Magnesium / Q000494:pharmacology; D008648:Mesoderm / Q000502:physiology; D009024:Morphogenesis; D010750:Phosphoproteins / Q000502:physiology; D050053:TRPM Cation Channels / Q000032:analysis / Q000502:physiology*; D029867:Xenopus Proteins / Q000032:analysis / Q000502:physiology*; D014982:Xenopus laevis / Q000196:embryology*; D020744:rac GTP-Binding Proteins / Q000502:physiology
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C000606586:DVL1 protein, Xenopus; D000072261:Dishevelled Proteins; D010750:Phosphoproteins; D050053:TRPM Cation Channels; C556687:TRPM7 protein, Xenopus; D029867:Xenopus Proteins; D020744:rac GTP-Binding Proteins; D008274:Magnesium