title: Affibody-based nanoprobes for HER2-expressing cell and tumor imaging.
abstract: This article reports the affibody-based nanoprobes specifically target and image human epidermal growth factor receptor type 2 (HER2)-expressing cells and tumors. The affibody molecules are a promising class of targeting ligands with simple, robust, and precise structure and high affinity. Using near-infrared (NIR) quantum dots (QDs) and iron oxide (IO) nanoparticles as two representative nanomaterials, we designed anti-HER2 affibody molecules with a N-terminus cysteine residue (Cysteine-Z(HER2:342)) and precisely conjugated with maleimide-functionalized nanoparticles to make nanoparticle-affibody conjugates. The in vitro and in vivo study showed the conjugates are highly specific to target and image HER2-expressing cells and tumors. This work indicated the nanoparticle-affibody conjugates may be excellent candidates as targeting probes for molecular imaging and diagnosis.
authors.affiliation: Molecular Imaging Program at Stanford (MIPS), Canary Center at Stanford for Cancer Early Detection, Department of Radiology and Bio-X Program, School of Medicine, Stanford University, 1201 Welch Road, Lucas P095, Stanford, CA 94305-5484, USA.
authors.forename: Jinhao
authors.initials: J
authors.lastname: Gao
authors.forename: Kai
authors.initials: K
authors.lastname: Chen
authors.forename: Zheng
authors.initials: Z
authors.lastname: Miao
authors.forename: Gang
authors.initials: G
authors.lastname: Ren
authors.forename: Xiaoyuan
authors.initials: X
authors.lastname: Chen
authors.forename: Sanjiv S
authors.initials: SS
authors.lastname: Gambhir
authors.forename: Zhen
authors.initials: Z
authors.lastname: Cheng
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D008279:Magnetic Resonance Imaging; D008297:Male; D053768:Metal Nanoparticles / Q000737:chemistry*; D051379:Mice; D008819:Mice, Nude; D008856:Microscopy, Fluorescence / Q000379:methods; D008958:Models, Molecular; D057054:Molecular Imaging / Q000379:methods*; D009369:Neoplasms / Q000378:metabolism* / Q000473:pathology; D011092:Polyethylene Glycols / Q000737:chemistry; D045663:Quantum Dots; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism*; D014018:Tissue Distribution
chemical_list: D011092:Polyethylene Glycols; D018719:Erb-b2 Receptor Tyrosine Kinases; D011993:Recombinant Fusion Proteins; C533351:affibody (ZHER2-342)2