title: [Prevalence of HPV 16, 18, 45 and 31 in women with cervical lesions].
abstract: OBJECTIVE
  To determine the prevalence of HPV 16, 18, 31 and 45 in cervical screening samples of women with cellular changes and/or colposcopy suggestive of persistent high grade or low grade lesion who were submitted to conization.
  
  
  METHODS
  A total of 120 women were included in the study. Histological analysis of the cervical cones revealed 7 cases of cervicitis, 22 of CIN1, 31 of CIN2, 54 of CIN3, and 6 invasive carcinomas. The cervical screening samples were analyzed before conization for the presence of HPV-DNA by PCR using the consensus primers PGMY09/11. HPV-DNA-positive samples were tested for the presence of HPV16, 18, 31 and 45 using type-specific primers for these HPV.
  
  
  RESULTS
  HPV-DNA was detected in 67.5% of the studied women. HPV 16 (40%) was the most prevalent type in most ilesions, followed by HPV 31 (13.3%), 45 (13.3%), and 18 (4.1%). Multiple infections occurred in 15% of the cases and infections with other HPV types were detected in 14% of the sample.
  
  
  CONCLUSIONS
  HPV 16 and 18 infections do not always occur as a single infection, and may be associated with other HPV types on different occasions.
authors.affiliation: Departamento de Tocoginecologia, Centro de Atenção Integral à Saúde, Universidade Estadual de Campinas, SP, Brasil.
authors.forename: Denise Rocha
authors.initials: DR
authors.lastname: Pitta
authors.forename: Elisabete Aparecida
authors.initials: EA
authors.lastname: Campos
authors.forename: Luis Otávio
authors.initials: LO
authors.lastname: Sarian
authors.forename: Marcello Silveira
authors.initials: MS
authors.lastname: Rovella
authors.forename: Sophie Françoise Mauricette
authors.initials: SF
authors.lastname: Derchain
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D005260:Female; D052162:Human papillomavirus 16; D052161:Human papillomavirus 18; D058952:Human papillomavirus 31; D006801:Humans; D008875:Middle Aged; D030361:Papillomavirus Infections / Q000453:epidemiology*; D015995:Prevalence; D002583:Uterine Cervical Neoplasms / Q000453:epidemiology* / Q000821:virology*; D055815:Young Adult