title: Cohesin organizes chromatin loops at DNA replication factories.
abstract: Genomic DNA is packed in chromatin fibers organized in higher-order structures within the interphase nucleus. One level of organization involves the formation of chromatin loops that may provide a favorable environment to processes such as DNA replication, transcription, and repair. However, little is known about the mechanistic basis of this structuration. Here we demonstrate that cohesin participates in the spatial organization of DNA replication factories in human cells. Cohesin is enriched at replication origins and interacts with prereplication complex proteins. Down-regulation of cohesin slows down S-phase progression by limiting the number of active origins and increasing the length of chromatin loops that correspond with replicon units. These results give a new dimension to the role of cohesin in the architectural organization of interphase chromatin, by showing its participation in DNA replication.
authors.affiliation: DNA Replication Group, Spanish National Cancer Research Centre (CNIO), E-28029 Madrid, Spain.
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Guillou
authors.forename: Arkaitz
authors.initials: A
authors.lastname: Ibarra
authors.forename: Vincent
authors.initials: V
authors.lastname: Coulon
authors.forename: Juan
authors.initials: J
authors.lastname: Casado-Vela
authors.forename: Daniel
authors.initials: D
authors.lastname: Rico
authors.forename: Ignacio
authors.initials: I
authors.lastname: Casal
authors.forename: Etienne
authors.initials: E
authors.lastname: Schwob
authors.forename: Ana
authors.initials: A
authors.lastname: Losada
authors.forename: Juan
authors.initials: J
authors.lastname: Méndez
mesh_terms: D018797:Cell Cycle Proteins / Q000032:analysis / Q000502:physiology*; D002460:Cell Line; D002843:Chromatin / Q000737:chemistry*; D002868:Chromosomal Proteins, Non-Histone / Q000032:analysis / Q000502:physiology*; D042003:DNA Packaging; D004261:DNA Replication; D006801:Humans; D007399:Interphase; D018741:Replication Origin; D016196:S Phase; D000097722:Cohesins
chemical_list: D018797:Cell Cycle Proteins; D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D000097722:Cohesins