title: [Redox modulation of large conductance calcium-activated potassium channels in rat cultured trigeminal ganglion neurons].
abstract: OBJECTIVE
  To observe redox modulation of ion channel in trigeminal ganglion neurons by oxidants and reducing agents.
  
  
  METHODS
  The effects of oxidants and reducing agents on maxi-conductance calcium-activated potassium channel in cultured rat trigeminal ganglion neurons by using whole-cell patch-clamp technique.
  
  
  RESULTS
  Methionine-specific oxidant chloramine-T (Ch-T) 1 mmol/L slightly increased the current amplitude and this enhancement did not antagonized by DTT. In contrast, cysteine-specific reagent 5, 5'-dithio-bis(2-nitrobenzoic acid) (DTNB) 500 micromol/L significantly decreased current amplitude of BK(Ca) channels. The effect was reversed by the reducing agent 2 mmol/L 1, 4-dithio-DL-threitol (DTT).
  
  
  CONCLUSIONS
  Reactive oxygen species were definitely involved in regulation of native neuronal function via redox modulation of BK(Ca) channels, which are suggested to play compensatory roles under oxidative stress-related conditions.
authors.affiliation: Department of Pharmacology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Zhong-Ling
authors.initials: ZL
authors.lastname: Zhu
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.forename: Zhou-Huan
authors.initials: ZH
authors.lastname: Wu
authors.forename: Li-Hong
authors.initials: LH
authors.lastname: Long
authors.forename: You
authors.initials: Y
authors.lastname: Jin
authors.forename: Jian-Guo
authors.initials: JG
authors.lastname: Chen
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D051036:Large-Conductance Calcium-Activated Potassium Channels / Q000502:physiology*; D008297:Male; D009474:Neurons / Q000378:metabolism / Q000502:physiology*; D010084:Oxidation-Reduction; D018408:Patch-Clamp Techniques; D051381:Rats; D017207:Rats, Sprague-Dawley; D012668:Trigeminal Ganglion / Q000166:cytology / Q000502:physiology*
chemical_list: D051036:Large-Conductance Calcium-Activated Potassium Channels