title: Multiple 100 Hz electroacupuncture treatments produced cumulative effect on the suppression of morphine withdrawal syndrome: Central preprodynorphin mRNA and p-CREB implicated.
abstract: Alleviating opiate withdrawal syndrome in addicts is a critical precondition to break away from drug and further to prevent reuse. Electroacupuncture (EA) was claimed to be effective for alleviating withdrawal syndrome, but the optimal protocol remained unclear. In the present study we found that (1) 100 Hz EA administered 12-24h after the last morphine injection suppressed the withdrawal syndrome in rats, multiple sessions of EA were more effective than single session, with the after-effect lasting for at least 7 days. (2) A down-regulation of preprodynorphin (PPD) mRNA level was observed in spinal cord, PAG and hypothalamus 60 h after the last morphine injection, which could be reversed by multiple sessions, but not a single session of EA. (3) Accompanied with the decrease of PPD mRNA level, there was an up-regulation of p-CREB in the three CNS regions, which was abolished by 100 Hz EA treatment. The findings suggest that down-regulation of p-CREB and acceleration of dynorphin synthesis in spinal cord, PAG and hypothalamus may be implicated in the cumulative effect of multiple 100Hz EA treatment for opioid detoxification.
authors.affiliation: Neuroscience Research Institute, Peking University and Department of Neurobiology, Peking University Health Science Center; Key Laboratory of Neuroscience of the Ministry of Education and the Ministry of Public Health, 38 Xueyuan Road, Beijing 100191, PR China.
authors.forename: Gui-Bin
authors.initials: GB
authors.lastname: Wang
authors.forename: Liu-Zhen
authors.initials: LZ
authors.lastname: Wu
authors.forename: Peng
authors.initials: P
authors.lastname: Yu
authors.forename: Yi-Jing
authors.initials: YJ
authors.lastname: Li
authors.forename: Xing-Jie
authors.initials: XJ
authors.lastname: Ping
authors.forename: Cai-Lian
authors.initials: CL
authors.lastname: Cui
mesh_terms: D000818:Animals; D001483:Base Sequence; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism*; D017931:DNA Primers; D004399:Dynorphins / Q000235:genetics*; D015671:Electroacupuncture; D008297:Male; D009020:Morphine / Q000009:adverse effects*; D010766:Phosphorylation; D011498:Protein Precursors / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013375:Substance Withdrawal Syndrome / Q000628:therapy*
chemical_list: D017362:Cyclic AMP Response Element-Binding Protein; D017931:DNA Primers; D011498:Protein Precursors; D012333:RNA, Messenger; C036448:pre-prodynorphin; D004399:Dynorphins; D009020:Morphine