title: Delayed recovery of myocardial blood flow after intracoronary stem cell administration.
abstract: The aim of the present study was to investigate the changes in absolute myocardial blood flow (AMF) after intracoronary injections of mesenchymal SC (MSC) and compared to controls in closed-chest reperfused acute myocardial infarction (AMI) in pigs. Male MSCs, transiently transfected with Luciferase (Luc-MSC) were delivered (9.7 ± 1.2 x 10(6)) intracoronary in the open infarct-related artery one-week post-AMI in female pigs (group MSC), while saline was injected with the same injection rate in controls (group C). The AMF was measured immediately after, and 3, 12 and 24 h post-intracoronary Luc-MSC or saline injections. In vitro bioluminescence images and quantitative real-time TaqMan PCR measurements were performed to quantify the sex-mismatched MSCs. No difference between the groups was observed regarding the weight, heart rate, blood pressure and global ejection fraction 1-week post-AMI. The baseline AMF were similar in the groups (61.3 ± 15. vs 61.1 ± 12.0 ml/min). AMF was decreased significantly immediately after intracoronary MSC delivery (42.0 ± 12.4 vs 57.7 ± 15.7 ml/min p = 0.013), and remained low at 3 h (40.9 ± 13.4 vs 55.8 ± 4.9 ml/min, p = 0.004), 12 h (43.0 ± 3.7 vs 57.8 ± 5.4 ml/min, p = 0.001) with incomplete recovery at 24 h (47.2 ± 5.5 vs 62.1 ± 14.1 ml/min, p = 0.038) as compared to controls, respectively. In vitro bioluminescence displayed transfected Luc-MSCs along the proximal and mid part of the LAD, with limited number (295 ± 101 sry copied/million cardiac cells) of Y-chromosome-MSCs in the infarcted area. Intracoronary injection of SCs results in immediate decrease of AMF, with delayed recovery. The delivery of the SC into the injured myocardium might be hindered by the altered coronary pressure and flow conditions.
authors.affiliation: Department of Cardiology, Medical University of Vienna, Währinger Gürtel 18-20, 1090, Vienna, Austria. mariann.gyongyosi@meduniwien.ac.at
authors.forename: Mariann
authors.initials: M
authors.lastname: Gyöngyösi
authors.forename: Rayyan
authors.initials: R
authors.lastname: Hemetsberger
authors.forename: Susanne
authors.initials: S
authors.lastname: Wolbank
authors.forename: Valentin
authors.initials: V
authors.lastname: Pichler
authors.forename: Christoph
authors.initials: C
authors.lastname: Kaun
authors.forename: Aniko
authors.initials: A
authors.lastname: Posa
authors.forename: Zsolt
authors.initials: Z
authors.lastname: Petrasi
authors.forename: Örs
authors.initials: Ö
authors.lastname: Petnehazy
authors.forename: Renate
authors.initials: R
authors.lastname: Hofer-Warbinek
authors.forename: Rainer
authors.initials: R
authors.lastname: de Martin
authors.forename: Florian
authors.initials: F
authors.lastname: Gruber
authors.forename: Imre
authors.initials: I
authors.lastname: Benedek
authors.forename: Teodora
authors.initials: T
authors.lastname: Benedek
authors.forename: Istvan
authors.initials: I
authors.lastname: Kovacs
authors.forename: Istvan
authors.initials: I
authors.lastname: Benedek
authors.forename: Christian A
authors.initials: CA
authors.lastname: Plass
authors.forename: Silvia
authors.initials: S
authors.lastname: Charwat
authors.forename: Gerald
authors.initials: G
authors.lastname: Maurer
mesh_terms: D000818:Animals; D064987:Cell- and Tissue-Based Therapy / Q000379:methods; D003326:Coronary Circulation / Q000502:physiology*; D005260:Female; D006439:Hemodynamics; D006801:Humans; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology; D009203:Myocardial Infarction / Q000628:therapy*; D009206:Myocardium / Q000166:cytology; D011897:Random Allocation; D012039:Regional Blood Flow; D013552:Swine