title: [The changes of resting [Ca2+]i level and expression of CaM mRNA, CaMPK II mRNA in hippocampal neurons of the mice with vascular dementia].
abstract: OBJECTIVE
  To investigate the resting [Ca2]i level and expression of calmodulin (CaM), calmodulin-dependent protein kinase II (CaMPKII) mRNA in hippocampal neurons of the mice with vascular dementia (VD) and their roles in the pathogenesis of VD.
  
  
  METHODS
  The mice were subjected for ischemia/reperfusion repeatedly on bilateral common carotid arteries by knots to establish the VD models. Animals with the sham-operation were taken as control group. The changes of behavior were observed through the step-down avoidance test and water maze test on the day 29, 30 after the operations. The hippocampal neurons were obtained immediately after mice were sacrificed and the resting [Ca2+]i was measured using laser scanning confocal microscopy with Fluo-3/AM as fluorescence indicator. RT-PCR technique was used to measure the mRNA expression of CaM, CaMPKII in hippocampal neurons.
  
  
  RESULTS
  (1) The abilities of learning and memorizing in model group were inferior to those of sham-operation group( P < 0.05). (2) The resting [Ca2]i level in model group was significantly higher than sham-operation group (P < 0.05), while the expression of CaMmRNA, CaMPKIImRNA in VD group was significantly reduced than sham-operation group (P < 0.01).
  
  
  CONCLUSIONS
  Our study indicates that excessive resting[Ca2+ ]i level and lower CaM, CaMPKII expression in hippocampal neurons might participate in the pathogenesis of VD.
authors.affiliation: Department of Neurology, Hebei Provincial People's Hospital, Shijiazhuang 050051, China.
authors.forename: Pei-yuan
authors.initials: PY
authors.lastname: Lu
authors.forename: Wen-bin
authors.initials: WB
authors.lastname: Li
authors.forename: Yu
authors.initials: Y
authors.lastname: Yin
authors.forename: Wei-bin
authors.initials: WB
authors.lastname: Wang
authors.forename: Cui-ping
authors.initials: CP
authors.lastname: Liang
mesh_terms: D000818:Animals; D002118:Calcium / Q000032:analysis*; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2 / Q000235:genetics / Q000378:metabolism*; D002147:Calmodulin / Q000235:genetics / Q000378:metabolism*; D015140:Dementia, Vascular / Q000378:metabolism*; D006624:Hippocampus / Q000166:cytology* / Q000378:metabolism; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009474:Neurons / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D002147:Calmodulin; D012333:RNA, Messenger; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; D002118:Calcium