title: [Activity and isoenzyme spectra of lactate dehydrogenase of plateau zokor (Myospalax baileyi) in different season].
abstract: OBJECTIVE
  To explore the adapting metabolic mechanisms of the plateau zokors to the hypoxic-hypercapnic environment.
  
  
  METHODS
  The activities of lactate dehydrogenase in serum and tissues, and the content of lactate in serum of plateau zokors in spring, summer and autumn were determined by using method of enzyme analysis. The spectrums of lactate dehydrogenase isoenzymes in serum and tissues of plateau zokors in spring, summer and autumn were analyzed by using method of the discontinuous systemic poly-acrylamide perpendicular plank gel electrophoresis.
  
  
  RESULTS
  The activities of lactate dehydrogenase in serum had obvious seasonally difference that were higher in spring and lower in autumn, and the content of lactate in serum showed same changing pattern. The spectrums of lactate dehydrogenase isoenzymes in serum showed five bands that were LDH1, LDH2, LDH3, LDH4 and LDH5 from positive pole to negative pole respectively, it showed clearly two bands in serum of summer that were LDH4 and LDH5 and one band in serum of autumn that was LDH5. The activities of LDH in tissues of skeleton muscle, cardiac muscle and brain were higher compared with the other tissues, it decreased markedly from spring to summer to autumn. In tissues of liver, kidney and lungs, activities of LDH were lower. Activities of LDH in livers, were significantly higher in spring compared that in summer and autumn, which had no obvious difference between summer and autumn. Activities of LDH in kidneys and lungs, showed no obviously difference between spring and summer, which decreased markedly in autumn. The spectrums of lactate dehydrogenase isoenzymes in tissues of cardiac muscle, liver, lungs, kidney, brain and skeleton muscle showed five bands, the spectrums were obvious different in different tissues, and the content of LDH isoenzymes showed seasonal changes in different tissues.
  
  
  CONCLUSIONS
  Glycolysis levels in plateau zokors had obvious seasonally change which increased in spring and decreased in autumn significantly. It related to the activity of plateau zokors in different seasons and seasonal fluctuation of oxygen and carbon dioxide in burrows of plateau zokors.
authors.affiliation: Department of Biology Sciences, Qinghai University, Xining 810016, China. weidengbang@163.com
authors.forename: Deng-Bang
authors.initials: DB
authors.lastname: Wei
authors.forename: Hong-Yan
authors.initials: HY
authors.lastname: Yu
authors.forename: Jian-Mei
authors.initials: JM
authors.lastname: Zhang
authors.forename: Xiao-Jun
authors.initials: XJ
authors.lastname: Wang
authors.forename: Lian
authors.initials: L
authors.lastname: Wei
mesh_terms: D000818:Animals; D002245:Carbon Dioxide / Q000378:metabolism; D007527:Isoenzymes / Q000032:analysis / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000032:analysis / Q000378:metabolism*; D000080864:Lactate Dehydrogenase 5; D012377:Rodentia / Q000378:metabolism*; D012621:Seasons
chemical_list: D007527:Isoenzymes; D002245:Carbon Dioxide; D007770:L-Lactate Dehydrogenase; C409676:lactate dehydrogenase 2; C409677:lactate dehydrogenase 3; C409678:lactate dehydrogenase 4; D000080864:Lactate Dehydrogenase 5; C409667:lactate dehydrogenase 1