title: Screening for DNA copy number aberrations in mucinous adenocarcinoma arising from the minor salivary gland: two case reports.
abstract: Mucinous adenocarcinoma (MAC) is a rare malignancy in the minor salivary gland. To our knowledge, genomic alterations in this tumor have not been reported previously. To identify DNA copy number aberrations, we applied comparative genomic hybridization (CGH) to four samples of MAC in minor salivary gland derived from two patients: a primary tumor and two cervical metastatic lymph nodes from one patient, and a primary tumor from the other patient. Copy number increases were commonly detected in 1q21∼q31 and 20q13, and these may play an important role in MAC carcinogenesis. Copy number increases in 1q, 12p, 12q, and 20q were commonly detected in all three samples derived from patient 1, and gain of 7p and loss of chromosome 4 were additionally detected in the two samples derived from metastatic lymph nodes. Amplifications were also detected in the chromosomal regions 8q22∼qter, 12p11∼p12, 12q11∼q21, and 20q13. Amplification of MDM2 (12q15) and of AURKA (20q13) was detected with fluorescence in situ hybridization. The DNA copy number aberrations detected in MAC in minor salivary glands were different from those reported for colorectal MAC. The present findings are novel in identifying genomic alterations of MAC arising from the minor salivary gland.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Yamaguchi University School of Medicine, 1-1-1 Minami-Kogushi, Ube, Yamaguchi 755-8505, Japan. k.uchida@yamaguchi-u.ac.jp
authors.forename: Kenichiro
authors.initials: K
authors.lastname: Uchida
authors.forename: Atsunori
authors.initials: A
authors.lastname: Oga
authors.forename: Takamitsu
authors.initials: T
authors.lastname: Mano
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Nagatsuka
authors.forename: Yoshiya
authors.initials: Y
authors.lastname: Ueyama
authors.forename: Kohsuke
authors.initials: K
authors.lastname: Sasaki
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics*; D001483:Base Sequence; D016022:Case-Control Studies; D022202:Chromosomes, Artificial, Bacterial; D017353:Gene Deletion; D018628:Gene Dosage; D005822:Genetic Vectors; D015894:Genome, Human; D006579:Heterozygote; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D009077:Mucins / Q000737:chemistry; D016133:Polymerase Chain Reaction; D015203:Reproducibility of Results; D012468:Salivary Gland Neoplasms / Q000473:pathology*; D012470:Salivary Glands, Minor / Q000473:pathology*
chemical_list: D009077:Mucins