title: [Effects of linoleic acid on intracellular calcium concentration in primarily cultured rat pancreatic β-cells and underlying mechanism].
abstract: In this study, we investigated the mechanism of linoleic acid-stimulated increase in intracellular calcium concentration ([Ca(2+)](i)) in pancreatic islet β-cells. Pancreatic islet cells were primarily isolated from rats and cultured for the experiments. The cells were loaded with Fluo-3/AM, the indicator of [Ca(2+)](i), and the intensity of Fluo-3 was measured using confocal microscope. The islet β-cells were identified by immunocytochemical staining with insulin antibody after recording. The drugs were given by perfusion system. The results showed that linoleic acid (20 μmol/L) stimulated [Ca(2+)](i) increase with the first peak increase and the following plateau increase. Linoleic acid-stimulated [Ca(2+)](i) increase was partly inhibited by removal of extracellular calcium and by transient receptor potential (TRP) channel blocker, La(3+), and it was totally blocked by exhaustion of intracellular calcium stores and inhibition of phospholipase C. It is concluded that linoleic acid stimulates [Ca(2+)](i) increase in islet β-cells through both extracellular calcium influx via TRP channels and calcium release from intracellular calcium stores.
authors.affiliation: The Second Affiliated Hospital of Medical School, Xi'an Jiao Tong University, Xi'an 710004, China.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.forename: Rong-Guo
authors.initials: RG
authors.lastname: Fu
authors.forename: Xiao-Dan
authors.initials: XD
authors.lastname: Liu
authors.forename: Bao-Song
authors.initials: BS
authors.lastname: Gui
authors.forename: Qiang
authors.initials: Q
authors.lastname: Sun
authors.forename: Chen
authors.initials: C
authors.lastname: Chen
authors.forename: Yu-Feng
authors.initials: YF
authors.lastname: Zhao
authors.forename: Lei
authors.initials: L
authors.lastname: Dong
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D050417:Insulin-Secreting Cells / Q000166:cytology* / Q000378:metabolism*; D019787:Linoleic Acid / Q000494:pharmacology*; D008297:Male; D061251:Primary Cell Culture; D051381:Rats; D017207:Rats, Sprague-Dawley; D050051:Transient Receptor Potential Channels / Q000037:antagonists & inhibitors
chemical_list: D050051:Transient Receptor Potential Channels; D019787:Linoleic Acid; D002118:Calcium