title: Over-expression of the triploid white poplar PtDrl01 gene in tobacco enhances resistance to tobacco mosaic virus.
abstract: A full-length cDNA, designated as the Populus tomentosa disease resistance-like 01 (PtDrl01) gene, was isolated from triploid white poplar [(Populus tomentosa × P. bolleana) × P. tomentosa]. The protein thought to be produced by the PtDrl01 gene contains a nuclear localisation sequence (NLS), a toll/interleukin-1 receptor (TIR) homologue region, a nucleotide binding site (NBS) and a leucine-rich repeat (LRR) domain. The protein also exhibits a considerable degree of homology to N-like resistance proteins. Real-time quantitative RT-PCR analysis revealed that expression of the PtDrl01 gene in triploid white poplar leaves could be induced by two defence signalling molecules: methyl jasmonate (MeJA) and salicylic acid (SA). Over-expression of the PtDrl01 gene in transgenic tobacco induced enhanced resistance to tobacco mosaic virus (TMV). Long-term resistance from the PtDrl01 gene to TMV infection was also observed in transgenic tobacco plants. Additionally, over-expression of the PtDrl01 gene resulted in transcriptional changes in genes expressing pathogenesis-related proteins in transgenic tobacco under non-stress conditions. These data strongly suggest that the PtDrl01 gene is involved in plant defence responses to pathogen infection.
authors.affiliation: National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding of Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University, Beijing, China.
authors.forename: H-Q
authors.initials: HQ
authors.lastname: Zheng
authors.forename: Q
authors.initials: Q
authors.lastname: Zhang
authors.forename: H-X
authors.initials: HX
authors.lastname: Li
authors.forename: S-Z
authors.initials: SZ
authors.lastname: Lin
authors.forename: X-M
authors.initials: XM
authors.lastname: An
authors.forename: Z-Y
authors.initials: ZY
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D017124:Conserved Sequence; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D032107:Populus / Q000378:metabolism*; D016415:Sequence Alignment; D014026:Nicotiana / Q000737:chemistry / Q000235:genetics / Q000378:metabolism* / Q000821:virology*; D014027:Tobacco Mosaic Virus / Q000502:physiology*; D057885:Triploidy
chemical_list: D010940:Plant Proteins