title: Construction of cardiac tissue rings using a magnetic tissue fabrication technique.
abstract: Here we applied a magnetic force-based tissue engineering technique to cardiac tissue fabrication. A mixture of extracellular matrix precursor and cardiomyocytes labeled with magnetic nanoparticles was added into a well containing a central polycarbonate cylinder. With the use of a magnet, the cells were attracted to the bottom of the well and allowed to form a cell layer. During cultivation, the cell layer shrank towards the cylinder, leading to the formation of a ring-shaped tissue that possessed a multilayered cell structure and contractile properties. These results indicate that magnetic tissue fabrication is a promising approach for cardiac tissue engineering.
authors.affiliation: Department of Chemical Engineering, Faculty of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan; E-Mails: hakiyama@chem-eng.kyushu-u.ac.jp (H.A.); akira@chem-eng.kyushu-u.ac.jp (A.I.); 2TE10138G@s.kyushu-u.ac.jp (M.S.); kawabe@chem-eng.kyushu-u.ac.jp (Y.K.).
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Akiyama
authors.forename: Akira
authors.initials: A
authors.lastname: Ito
authors.forename: Masanori
authors.initials: M
authors.lastname: Sato
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Kawabe
authors.forename: Masamichi
authors.initials: M
authors.lastname: Kamihira
mesh_terms: D000818:Animals; D058185:Magnetite Nanoparticles; D009206:Myocardium / Q000166:cytology*; D032383:Myocytes, Cardiac / Q000166:cytology / Q000502:physiology; D051381:Rats; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D058185:Magnetite Nanoparticles