title: Interrelationships between mitochondrial fusion, energy metabolism and oxidative stress during development in Caenorhabditis elegans.
abstract: Mitochondria are known to be dynamic structures with the energetically and enzymatically mediated processes of fusion and fission responsible for maintaining a constant flux. Mitochondria also play a role of reactive oxygen species production as a byproduct of energy metabolism. In the current study, interrelationships between mitochondrial fusion, energy metabolism and oxidative stress on development were explored using a fzo-1 mutant defective in the fusion process and a mev-1 mutant overproducing superoxide from mitochondrial electron transport complex II of Caenorhabditis elegans. While growth and development of both single mutants was slightly delayed relative to the wild type, the fzo-1;mev-1 double mutant experienced considerable delay. Oxygen sensitivity during larval development, superoxide production and carbonyl protein accumulation of the fzo-1 mutant were similar to wild type. fzo-1 animals had significantly lower metabolism than did N2 and mev-1. These data indicate that mitochondrial fusion can profoundly affect energy metabolism and development.
authors.affiliation: Department of Molecular Life Science, Tokai University School of Medicine, Isehara, Kanagawa 259-1193, Japan.
authors.forename: Kayo
authors.initials: K
authors.lastname: Yasuda
authors.forename: Philip S
authors.initials: PS
authors.lastname: Hartman
authors.forename: Takamasa
authors.initials: T
authors.lastname: Ishii
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Suda
authors.forename: Akira
authors.initials: A
authors.lastname: Akatsuka
authors.forename: Tetsuji
authors.initials: T
authors.lastname: Shoyama
authors.forename: Masaki
authors.initials: M
authors.lastname: Miyazawa
authors.forename: Naoaki
authors.initials: N
authors.lastname: Ishii
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000254:growth & development*; D029742:Caenorhabditis elegans Proteins / Q000378:metabolism; D045303:Cytochromes b; D004734:Energy Metabolism; D020558:GTP Phosphohydrolases / Q000378:metabolism; D008561:Membrane Fusion; D008928:Mitochondria / Q000502:physiology*; D018384:Oxidative Stress; D050050:Protein Carbonylation; D013385:Succinate Dehydrogenase / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism
chemical_list: D029742:Caenorhabditis elegans Proteins; C472666:MEV-1 protein, C elegans; D013481:Superoxides; D045303:Cytochromes b; D013385:Succinate Dehydrogenase; C543814:FZO-1 protein, C elegans; D020558:GTP Phosphohydrolases