title: [Determination of homocysteine level in plasma by high performance liquids chromatography with fluorescence detection].
abstract: OBJECTIVE
  To establish a method for detection of plasma total homocysteine with HPLC.
  
  
  METHODS
  The chromatography analysis was carried out using a Symmetry Shield RP18. The mobile phase was sodium acetate (0.08 mol/L) and methanol (1%) and we utilized a HPLC system with fluorescence detection of plasma homocysteine derivatized from reaction with 7-fluorobenzo-2-oxa-1,3-diazole-4-sulphonate (SBD-F).
  
  
  RESULTS
  The average recoveries were 95.8 - 100.8% and the relative standard deviations were 1.2-2.0%.
  
  
  CONCLUSIONS
  The results showed it to be a rapid and accurate method for the determination of homocysteine level in plasma.
authors.affiliation: Institute of Health and Environmental Medicine, Academy of Military Medical Sciences, Tianjin 300050, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Da
authors.forename: Ling-Jia
authors.initials: LJ
authors.lastname: Qian
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006710:Homocysteine / Q000097:blood*; D006801:Humans; D010949:Plasma / Q000737:chemistry; D013050:Spectrometry, Fluorescence
chemical_list: D006710:Homocysteine