title: Novel multiplex real-time PCR system using the SNP technology for the simultaneous diagnosis of Chlamydia trachomatis, Ureaplasma parvum and Ureaplasma urealyticum and genetic typing of serovars of C. trachomatis and U. parvum in NGU.
abstract: To explore the possibilities of a novel multiplex real-time PCR system for rapid diagnosis, genetic typing of serovars and clinical application in NGU, we developed a multiplex real-time PCR system for the simultaneous diagnosis of Chlamydia trachomatis, Ureaplasma parvum and Ureaplasma urealyticum and molecular detection of serovars of C. trachomatis and U. parvum in NGU using the SNP technology and TaqMan-LNA probe. In 57 pathogen-positive clinical specimens, we identified the following C. trachomatis serovars: D (20.05%, 12/57), E (36.84%, 21/57), F (19.30%, 11/57), G (8.77%, 5/57), H (5.26%, 3/57), J (3.51%, 2/57), and K (5.26%, 3/57). In 115 pathogen-positive clinical specimens, we identified the following U. parvum serovars: 1 (0.87%, 2/115), 3 (55.65%, 64/115), 6 (20.87%, 24/115) and 14 (21.74%, 25/115). Our fast pathogen diagnosis and serotyping assay using real-time TaqMan-LNA PCR may improve our ability to study the pathogenesis and epidemiology of NGU.
authors.affiliation: The State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, China.
authors.forename: Jingfeng
authors.initials: J
authors.lastname: Tang
authors.forename: Li
authors.initials: L
authors.lastname: Zhou
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Liu
authors.forename: Changming
authors.initials: C
authors.lastname: Zhang
authors.forename: Youyun
authors.initials: Y
authors.lastname: Zhao
authors.forename: Yefu
authors.initials: Y
authors.lastname: Wang
mesh_terms: D002690:Chlamydia Infections / Q000175:diagnosis* / Q000382:microbiology; D002692:Chlamydia trachomatis / Q000145:classification / Q000235:genetics*; D015342:DNA Probes; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D012703:Serotyping / Q000379:methods*; D014509:Ureaplasma / Q000145:classification / Q000235:genetics*; D016869:Ureaplasma Infections / Q000175:diagnosis* / Q000382:microbiology; D016990:Ureaplasma urealyticum / Q000145:classification / Q000235:genetics; D014526:Urethritis / Q000175:diagnosis*
chemical_list: D015342:DNA Probes