title: [Roles of NRF2 regulating gamma-glutamylcysteine synthetase in lung of rats with chronic obstructive pulmonary disease].
abstract: OBJECTIVE
  To investigate the expression and relationship of gamma-glutamylcysteine synthetase (gamma-GCS) and NF-E2-related factor2 (NRR2) in lung of rat with chronic obstructive pulmonary disease (COPD)in order to elucidate the possible important role of gamma-GCS and NRF2 in pathogenesis of COPD.
  
  
  METHODS
  The rat COPD model was established by intratracheal instillation of lipopolysaccharide twice and exposed to cigarette smoke daily. The gamma-GCS activity was measured, the expression of gamma-GCS mRNA in lung was examined by in situ hybridization (ISH) and reverse transcription-polymerase chain reaction (RT-PCR), the protein expressions of NRF2, gamma-GCS in lung were detected by immunohistochemical (IH) and Western blot respectively.
  
  
  RESULTS
  The gamma-GCS activity was higher in COPD group than that in control group. The expressions of gamma-GCS mRNA in COPD group was stronger than those in control group. ISH showed that the gamma-GCS mRNA was expressed in alveolar epithelium and bronchial smooth muscle cell in COPD. The protein expressions of NRF2, gamma-GCS were significantly higher than the control group. IH showed that NRF2, gamma-GCS proteins were expressed in alveolar and bronchial epithelium in the COPD group. There was a positive correlation between NRF2 and gamma-GCS and gamma-GCS mRNA.
  
  
  CONCLUSIONS
  NRF2 may play an important role in the mechanism of COPD oxidative stress vis up-regulation of gamma-GCS.
authors.affiliation: Department of Respiratory Medicine, Geriatric Hospital of Hunan Province, Changsha 410001, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Chen
authors.forename: Ai-Guo
authors.initials: AG
authors.lastname: Dai
authors.forename: Rui-Cheng
authors.initials: RC
authors.lastname: Hu
mesh_terms: D000818:Animals; D005721:Glutamate-Cysteine Ligase / Q000378:metabolism*; D008168:Lung / Q000378:metabolism*; D008297:Male; D051267:NF-E2-Related Factor 2 / Q000378:metabolism*; D018384:Oxidative Stress; D029424:Pulmonary Disease, Chronic Obstructive / Q000378:metabolism* / Q000503:physiopathology*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
chemical_list: D051267:NF-E2-Related Factor 2; C495637:Nfe2l2 protein, rat; D005721:Glutamate-Cysteine Ligase