title: Efficient utilization of rare variants for detection of disease-related genomic regions.
abstract: When testing association between rare variants and diseases, an efficient analytical approach involves considering a set of variants in a genomic region as the unit of analysis. One factor complicating this approach is that the vast majority of rare variants in practical applications are believed to represent background neutral variation. As a result, analyzing a single set with all variants may not represent a powerful approach. Here, we propose two alternative strategies. In the first, we analyze the subsets of rare variants exhaustively. In the second, we categorize variants selectively into two subsets: one in which variants are overrepresented in cases, and the other in which variants are overrepresented in controls. When the proportion of neutral variants is moderate to large we show, by simulations, that the both proposed strategies improve the statistical power over methods analyzing a single set with total variants. When applied to a real sequencing association study, the proposed methods consistently produce smaller p-values than their competitors. When applied to another real sequencing dataset to study the difference of rare allele distributions between ethnic populations, the proposed methods detect the overrepresentation of variants between the CHB (Chinese Han in Beijing) and YRI (Yoruba people of Ibadan) populations with small p-values. Additional analyses suggest that there is no difference between the CHB and CHD (Chinese Han in Denver) datasets, as expected. Finally, when applied to the CHB and JPT (Japanese people in Tokyo) populations, existing methods fail to detect any difference, while it is detected by the proposed methods in several regions.
authors.affiliation: Center of System Biomedical Sciences, University of Shanghai for Science and Technology, Shanghai, People's Republic of China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.forename: Yu-Fang
authors.initials: YF
authors.lastname: Pei
authors.forename: Jian
authors.initials: J
authors.lastname: Li
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Papasian
authors.forename: Hong-Wen
authors.initials: HW
authors.lastname: Deng
mesh_terms: D000483:Alleles; D002681:China; D003198:Computer Simulation; D030342:Genetic Diseases, Inborn / Q000208:ethnology* / Q000235:genetics*; D014644:Genetic Variation; D005828:Genetics, Population; D023281:Genomics; D006239:Haplotypes; D006801:Humans; D007564:Japan; D009010:Monte Carlo Method; D010641:Phenotype; D015203:Reproducibility of Results; D017422:Sequence Analysis, DNA / Q000379:methods; D014481:United States