title: A combined global and local approach to elucidate spatial organization of the Mycobacterial ParB-parS partition assembly.
abstract: Combining diverse sets of data at global (size, shape) and local (residue) scales is an emerging trend for elucidating the organization and function of the cellular assemblies. We used such a strategy, combining data from X-ray and neutron scattering with H/D-contrast variation and X-ray footprinting with mass spectrometry, to elucidate the spatial organization of the ParB-parS assembly from Mycobacterium tuberculosis. The ParB-parS participates in plasmid and chromosome segregation and condensation in predivisional bacterial cells. ParB polymerizes around the parS centromere(s) to form a higher-order assembly that serves to recruit cyto-skeletal ParA ATPases and SMC proteins for chromosome segregation. A hybrid model of the ParB-parS was built by combining and correlating computational models with experiment-derived information about size, shape, position of the symmetry axis within the shape, internal topology, DNA-protein interface, exposed surface patches, and prior knowledge. This first view of the ParB-parS leads us to propose how ParB spread on the chromosome to form a larger assembly.
authors.affiliation: Hauptman Woodward Institute, Buffalo, NY 14203, USA. bchaudhuri@hwi.buffalo.edu
authors.forename: Barnali N
authors.initials: BN
authors.lastname: Chaudhuri
authors.forename: Sayan
authors.initials: S
authors.lastname: Gupta
authors.forename: Volker S
authors.initials: VS
authors.lastname: Urban
authors.forename: Mark R
authors.initials: MR
authors.lastname: Chance
authors.forename: Rhijuta
authors.initials: R
authors.lastname: D'Mello
authors.forename: Lauren
authors.initials: L
authors.lastname: Smith
authors.forename: Kelly
authors.initials: K
authors.lastname: Lyons
authors.forename: Jessica
authors.initials: J
authors.lastname: Gee
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D002503:Centromere / Q000378:metabolism; D020090:Chromosome Segregation; D004269:DNA, Bacterial / Q000737:chemistry* / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000378:metabolism; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins