title: Bioremediation of fluorophenols by glycosylation with immobilized marine microalga amphidinium crassum.
abstract: Fluorophenols are used as agrochemicals and released into environment as pollutants. Cultured marine microalga Amphidinium crassum (Gymnodinium) glucosylated 2-fluorophenol (1), 3-fluorophenol (2), and 4-fluorophenol (3) to the corresponding β-D-glucosides, ie, 2-fluorophenyl β-D-glucoside (4, 60 μg/g cells), 3-fluorophenyl β-D-glucoside (5, 20 μg/g cells), and 4-fluorophenyl β-D-glucoside (6, 40 μg/g cells). On the other hand, 2-, 3-, and 4-fluorophenols were efficiently converted by immobilized A. crassum in sodium alginate gel to give their β-D-glucosides in higher yields (4: 140 μg/g cells; 5: 60 μg/g cells; 6: 100 μg/g cells). In repetitive batch use, the immobilized cells of A. crassum maintained the potential for the glucosylation of the substrate fluorophenol after 5 times of usage.
authors.affiliation: Department of Chemistry, Faculty of Medicine, Oita University, 1-1 Hasama-machi, Oita 879-5593, Japan.
authors.forename: Kei
authors.initials: K
authors.lastname: Shimoda
authors.forename: Hiroki
authors.initials: H
authors.lastname: Hamada