title: Effects of 3-aminobenzamide on unilateral testicular ischemia-reperfusion injury: what is the role of PARP inhibition?
abstract: The therapeutic effects of poly(adenosine diphosphate-ribose) polymerase inhibition by 3-aminobenzamide (3-AB) were investigated in testicular ischemia-reperfusion (I/R) injury, using sperm analysis and histopathological and biochemical examinations, including superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) activities and reduced glutathione (GSH) levels. Male rats were divided into 3 groups: sham (n = 12), I/R (n = 12), and I/R with 3-AB (I/R-3-AB) (n = 12). The left testicular artery was occluded for 1 h, followed by 24 h (for biochemical and histopathological examinations) and 30 days (for sperm analysis) of reperfusion. 3-AB treatment intraperitoneally 10 min prior to and 1 h after reperfusion increased the I/R-induced decrease in sperm motility in both testes and reduced the increased abnormal sperm rates in the ipsilateral testis. However, 3-AB treatment failed to prevent the I/R-induced decrease in sperm concentration in both testes. SOD and CAT activities did not change in any group. GSH-Px activity and GSH levels were increased by I/R. 3-AB treatment reversed the I/R-induced increase in GSH-Px activity, similar to the level in sham rats, but did not alter GSH levels. 3-AB treatment significantly increased the I/R-induced decrease in histopathologic score. In conclusion, 3-AB treatment has potential biochemical and histopathological benefits beyond improving sperm quality and may have the potential to decrease damage from testicular torsion.
authors.affiliation: Department of Pharmacology, Faculty of Medicine, Dicle University, Diyarbakir, Turkey. askinh@hotmail.com
authors.forename: Askin
authors.initials: A
authors.lastname: Hekimoglu
authors.forename: Zehra
authors.initials: Z
authors.lastname: Kurcer
authors.forename: Faruk
authors.initials: F
authors.lastname: Aral
authors.forename: Fusun
authors.initials: F
authors.lastname: Baba
authors.forename: Ahmet
authors.initials: A
authors.lastname: Atessahin
authors.forename: Fatih
authors.initials: F
authors.lastname: Sakin
mesh_terms: D000818:Animals; D001549:Benzamides / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D008297:Male; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015424:Reperfusion; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000201:enzymology / Q000517:prevention & control*; D013086:Spermatic Cord Torsion / Q000188:drug therapy; D013094:Spermatozoa / Q000187:drug effects / Q000473:pathology; D013482:Superoxide Dismutase / Q000378:metabolism; D013733:Testicular Diseases / Q000188:drug therapy / Q000201:enzymology / Q000517:prevention & control; D013737:Testis / Q000098:blood supply* / Q000187:drug effects* / Q000201:enzymology / Q000601:surgery
chemical_list: D001549:Benzamides; D004791:Enzyme Inhibitors; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; C025160:3-aminobenzamide; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D011065:Poly(ADP-ribose) Polymerases; D005978:Glutathione