title: Real-time PCR assay for Clostridium perfringens in broiler chickens in a challenge model of necrotic enteritis.
abstract: We compared ileal Clostridium perfringens quantification results produced by real-time PCR and culture-based methods in broiler chickens in a challenge model of necrotic enteritis. Assessment of the relative standard deviations (RSDs) revealed that the real-time PCR assay generated a smaller standard deviation and thus was more precise than the culture-based method. Linear regression analysis indicated that the bacterial counts of these two methods were highly correlated (R(2) = 0.845). We suggest that real-time PCR could be a replacement of the culture method for quantifying C. perfringens in the intestinal tracts of broiler chickens.
authors.affiliation: School of Environmental and Rural Science, University of New England, Armidale, NSW 2351, Australia. shubiao.wu@une.edu.au
authors.forename: Shu-Biao
authors.initials: SB
authors.lastname: Wu
authors.forename: Nicholas
authors.initials: N
authors.lastname: Rodgers
authors.forename: Mingan
authors.initials: M
authors.lastname: Choct
mesh_terms: D000818:Animals; D058491:Bacterial Load; D001431:Bacteriological Techniques; D002645:Chickens / Q000382:microbiology*; D003016:Clostridium perfringens / Q000235:genetics* / Q000302:isolation & purification; D003470:Culture Media; D004751:Enteritis / Q000382:microbiology / Q000662:veterinary*; D007082:Ileum / Q000382:microbiology* / Q000473:pathology; D007422:Intestines / Q000382:microbiology / Q000473:pathology; D016014:Linear Models; D009336:Necrosis / Q000382:microbiology / Q000662:veterinary; D016133:Polymerase Chain Reaction / Q000379:methods*; D011201:Poultry Diseases / Q000175:diagnosis / Q000382:microbiology*
chemical_list: D003470:Culture Media