title: Mammal cells double their total RNAs against diabetes, ischemia reperfusion and malaria-induced oxidative stress.
abstract: Total cellular RNA level is stable usually, although it may increase gradually during growth or decrease gradually under certain stressors. However, we found that mammal cell RNAs could be doubled within 24 h in response to free heme accumulation (ischemia reperfusion and malaria infection) or a high level of glucose treatment (diabetes). Clinical investigations in rats showed that pretreatment with heme (24 h for doubling total RNAs) alleviated oxidative damages caused by diabetes, and pretreatment with glucose (24 h for trebling total RNAs) alleviated oxidative damages caused by ischemia reperfusion or malaria infection. Therefore, this rapid RNA amplification may play an important role in mammal adaptation to diabetes, ischemia reperfusion and malaria infection-derived oxidative stress. This rapid RNA amplification is derived from glucose and heme, but not from their accompanying reactive oxygen species. Hexokinases endure glucose-derived reactive oxygen species accumulation but are not related glucose-derived RNA amplification. In contrast, the TATA box-binding protein (TBP) mediates all glucose- and heme-induced RNA amplification in mammal cells.
authors.affiliation: College of Resources and Environmental Sciences, Sichuan Agriculture University, Chengdu, China.
authors.forename: Zhong-Wei
authors.initials: ZW
authors.lastname: Zhang
authors.forename: Jian
authors.initials: J
authors.lastname: Cheng
authors.forename: Fei
authors.initials: F
authors.lastname: Xu
authors.forename: Ming
authors.initials: M
authors.lastname: Yuan
authors.forename: Jun-Bo
authors.initials: JB
authors.lastname: Du
authors.forename: Jing
authors.initials: J
authors.lastname: Shang
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.forename: Lei
authors.initials: L
authors.lastname: Du
authors.forename: Zi-Lin
authors.initials: ZL
authors.lastname: Li
authors.forename: Shu
authors.initials: S
authors.lastname: Yuan
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D003921:Diabetes Mellitus, Experimental / Q000235:genetics*; D005947:Glucose / Q000494:pharmacology*; D006418:Heme / Q000494:pharmacology*; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D016778:Malaria, Falciparum / Q000235:genetics*; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology*; D016133:Polymerase Chain Reaction; D012313:RNA / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000235:genetics*; D013395:Sucrose / Q000494:pharmacology; D013481:Superoxides / Q000378:metabolism
chemical_list: D013481:Superoxides; D006418:Heme; D013395:Sucrose; D012313:RNA; D006861:Hydrogen Peroxide; D005947:Glucose