title: [Preparation of clenbuterol monoclonal antibody with subtractive immunization method].
abstract: OBJECTIVE
  To obtain Clenbuterol monoclonal antibodies.
  
  
  METHODS
  Clenbuterol complete antigen was prepared with diazotization method. BALB/c mice was immunized with subtractive immunization, Clenbuterol monoclonal antibody was prepared with rule hybridoma technique.
  
  
  RESULTS
  The mice obtained tolerance to BSA by subtractive immunization. The rate of the hybridoma cell with positive reaction which had obtained was 8.2%, and the specific clenbuterol monoclonal antibody was obtained at last.
  
  
  CONCLUSIONS
  Monoclonal antibodies to micromolecule contaminant be prepared by subtractive immunization, could decrease the workload in the bolting of monoclonal antibodies, and increase the chance to obtain the antibody of expected.
authors.affiliation: Institute of Health and Environmental Medicine, Academy of military Medical Sciences, Tianjin Key Laboratory of Risk Assessment and Control for Environment and Food Safety, Tianjin 300050, China.
authors.forename: Xiao-Li
authors.initials: XL
authors.lastname: Li
authors.forename: Xiao-Fang
authors.initials: XF
authors.lastname: Li
authors.forename: Bao-An
authors.initials: BA
authors.lastname: Ning
authors.forename: Da-Cheng
authors.initials: DC
authors.lastname: Wu
authors.forename: Hong-Yong
authors.initials: HY
authors.lastname: Wang
authors.forename: Xiang
authors.initials: X
authors.lastname: Chen
authors.forename: Xin-Hua
authors.initials: XH
authors.lastname: Ma
authors.forename: Guo-Rong
authors.initials: GR
authors.lastname: Ou
authors.forename: Zhi-Xian
authors.initials: ZX
authors.lastname: Gau
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D002976:Clenbuterol / Q000276:immunology*; D005260:Female; D006825:Hybridomas / Q000378:metabolism; D007114:Immunization / Q000379:methods*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D012710:Serum Albumin, Bovine / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D012710:Serum Albumin, Bovine; D002976:Clenbuterol