title: Loss of BCL2L10 protein expression as prognostic predictor for poor clinical outcome in gastric carcinoma.
abstract: OBJECTIVE
  BCL2L10 protein is an apoptosis-related member of the Bcl-2 protein family. The clinical significance of its expression in gastric carcinoma is poorly understood. The aim was to investigate BCL2L10 expression and its clinical and prognostic significance in gastric carcinoma patients.
  
  
  RESULTS
  Immunohistochemistry, real-time polymerase chain reaction (PCR) and immunoblotting all revealed extensive loss of BCL2L10 expression in gastric cancer cells. The scaled BCL2L10 expression data was categorized into three groups (groups 0-2) to facilitate statistical analysis. A significant correlation was observed between the lower BCL2L10 expression group and shorter disease-free survival (P=1.956×10(-18)). Multivariate regression analysis showed that loss of BCL2L10 protein expression [P=4.883×10(-8), hazard ratio (HR)=0.252] is an independent prognostic predictor of gastric carcinoma. The receiver operator characteristic (ROC) curve showed that the area for BCL2L10 protein was 0.817 (P=8.331×10(-14)), indicating that loss of BCL2L10 protein expression is an excellent prognostic predictor of gastric carcinoma.
  
  
  CONCLUSIONS
  Loss of BCL2L10 protein expression predicts poor clinical outcome in gastric carcinoma.
authors.affiliation: Department of Pathology, Shanghai Medical College, Fudan University, Shanghai, China.
authors.forename: Jing Da
authors.initials: JD
authors.lastname: Xu
authors.forename: Tomoko
authors.initials: T
authors.lastname: Furuya
authors.forename: Xi Xi
authors.initials: XX
authors.lastname: Cao
authors.forename: Xiao Li
authors.initials: XL
authors.lastname: Liu
authors.forename: Qing Quan
authors.initials: QQ
authors.lastname: Li
authors.forename: Wen Juan
authors.initials: WJ
authors.lastname: Wang
authors.forename: Jia Wen
authors.initials: JW
authors.lastname: Xu
authors.forename: Zu De
authors.initials: ZD
authors.lastname: Xu
authors.forename: Kohsuke
authors.initials: K
authors.lastname: Sasaki
authors.forename: Xiu Ping
authors.initials: XP
authors.lastname: Liu
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D000368:Aged; D000369:Aged, 80 and over; D015153:Blotting, Western; D045744:Cell Line, Tumor; D018572:Disease-Free Survival; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D012044:Regression Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D016896:Treatment Outcome; D014407:Tumor Cells, Cultured
chemical_list: C117144:BCL2-like 10 protein; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger