title: [Measurement of systolic/diastolic function in isolated ventricular myocytes by video-based motion edge-detection system].
abstract: OBJECTIVE
  Single cardiomyocytes were isolated from adult rat hearts and myocyte contraction was recorded by a video-based motion edge-detection system.
  
  
  METHODS
  Single ventricular myocytes were enzymatically isolated, loaded with fura-2/AM (0.5 micromol/L) for 30 min in dark at room temperature and field-stimulated (0.5 Hz, 5 ms), and both myocyte contraction and intracellular fluorescence intensity were simultaneously assessed by a video-based motion edge-detection system.
  
  
  RESULTS
  Using the video-based motion edge-detection system, one may real-time beat-to-beat observe and record the amplitude, duration and velocity of both myocyte shortening/ relengthening and intracellular calcium transient, and then directly describe the change of isolated ventricular myocyte mechanics.
  
  
  CONCLUSIONS
  Direct measurement of isolated ventricular myocyte mechanics using the video-based motion edge-detection system is an increasingly important technique in cardiac physiology that provides fundamental information on myogenic and excitation-contraction coupling of the heart in drug intervention and gene manipulation.
authors.affiliation: Department of Physiology, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Hai-Feng
authors.initials: HF
authors.lastname: Zhang
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Zhang
authors.forename: Feng
authors.initials: F
authors.lastname: Gao
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D003584:Cytological Techniques; D006352:Heart Ventricles / Q000166:cytology; D009200:Myocardial Contraction; D032383:Myocytes, Cardiac / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D059010:Single-Cell Analysis; D014741:Video Recording / Q000295:instrumentation*
chemical_list: D002118:Calcium