title: Rotational position of a 5-methylcytosine-containing cyclobutane pyrimidine dimer in a nucleosome greatly affects its deamination rate.
abstract: C to T mutation hotspots in skin cancers occur primarily at methylated CpG sites that coincide with sites of UV-induced cyclobutane pyrimidine dimer (CPD) formation. These mutations are proposed to arise from the insertion of A by DNA polymerase η opposite the T that results from deamination of the methylC ((m)C) within the CPD. Although the frequency of CPD formation and repair is modestly modulated by its rotational position within a nucleosome, the effect of position on the rate of (m)C deamination in a CPD has not been previously studied. We now report that deamination of a T(m)C CPD whose sugar phosphate backbone is positioned against the histone core surface decreases by a factor of 4.7, whereas that of a T(m)C CPD positioned away from the surface increases by a factor of 8.9 when compared with unbound DNA. Because the (m)Cs undergoing deamination are in similar steric environments, the difference in rate appears to be a consequence of a difference in the flexibility and compression of the two sites due to DNA bending. Considering that formation of the CPD positioned away from the surface is also enhanced by a factor of two, a T(m)CG site in this position might be expected to have up to an 84-fold higher probability of resulting in a UV-induced (m)C to T mutation than one positioned against the surface. These results indicate that rotational position may play an important role in the formation of UV-induced C to T mutation hotspots, as well as in the mutagenic mechanism of other DNA lesions.
authors.affiliation: Department of Chemistry, Washington University, St. Louis, Missouri 63130, USA.
authors.forename: Qian
authors.initials: Q
authors.lastname: Song
authors.forename: Vincent J
authors.initials: VJ
authors.lastname: Cannistraro
authors.forename: John-Stephen
authors.initials: JS
authors.lastname: Taylor
mesh_terms: D044503:5-Methylcytosine / Q000737:chemistry* / Q000378:metabolism; D000818:Animals; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D003641:Deamination / Q000528:radiation effects; D008958:Models, Molecular; D016296:Mutagenesis / Q000528:radiation effects; D009707:Nucleosomes / Q000737:chemistry* / Q000378:metabolism; D011740:Pyrimidine Dimers / Q000737:chemistry* / Q000378:metabolism; D014466:Ultraviolet Rays; D014982:Xenopus laevis
chemical_list: D009707:Nucleosomes; D011740:Pyrimidine Dimers; D044503:5-Methylcytosine; D004247:DNA