title: Efficient conversion from polysialogangliosides to monosialotetrahexosylganglioside using Oerskovia xanthineolytica YZ-2.
abstract: A new sialidase-producing strain isolated from soil was identified as Oerskovia xanthineolytica YZ-2. Sialidase was produced when Oerskovia xanthineolytica YZ-2 was exposed to polysialogangliosides. The sialidase of Oerskovia xanthineolytica YZ-2 hydrolyzed sialic acid linkages in polysialogangliosides, and released monosialotetrahexosylganglioside (GM1). The sialidase had the capability of product specificity because it did not attack the sialic acid linkage in GM1. Therefore, Oerskovia xanthineolytica YZ-2 was used for GM1 production from polysialogangliosides. In flasks cultivation phase, it was proved that Oerskovia xanthineolytica YZ-2 could convert polysialogangliosides to GM1 efficiently. Scaling-up the bioprocess with 8% crude ganglioside, polysialogangliosides was converted to GM1 by Oerskovia xanthineolytica YZ-2 in 30 L bioreactor after 18 h. The relative content of GM1 increased from 16.3% in crude ganglioside to 83.7% after Oerskovia xanthineolytica YZ-2 conversion. Therefore, a simple, large-scale conversion process for GM1 production from polysialogangliosides was achieved using Oerskovia xanthineolytica YZ-2 as a biocatalyst.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, College of Biotechnology, East China University of Science and Technology, No. 130 Meilong Road, Shanghai, People's Republic of China.
authors.forename: Jianguo
authors.initials: J
authors.lastname: Zhang
authors.forename: Dan
authors.initials: D
authors.lastname: Cao
authors.forename: Danhong
authors.initials: D
authors.lastname: Shen
authors.forename: Xuedong
authors.initials: X
authors.lastname: Wang
authors.forename: Dongzhi
authors.initials: D
authors.lastname: Wei
mesh_terms: D000192:Actinomycetales / Q000378:metabolism; D000818:Animals; D019149:Bioreactors; D001709:Biotechnology / Q000379:methods; D002240:Carbohydrate Sequence; D003470:Culture Media / Q000737:chemistry; D005285:Fermentation; D005677:G(M1) Ganglioside / Q000737:chemistry; D005732:Gangliosides / Q000737:chemistry*; D006801:Humans; D008956:Models, Chemical; D008969:Molecular Sequence Data; D019158:N-Acetylneuraminic Acid / Q000737:chemistry*; D009439:Neuraminidase / Q000737:chemistry; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013552:Swine; D013696:Temperature
chemical_list: D003470:Culture Media; D005732:Gangliosides; D012336:RNA, Ribosomal, 16S; D005677:G(M1) Ganglioside; D009439:Neuraminidase; D019158:N-Acetylneuraminic Acid