title: Chromosomal alterations in Malaysian patients with nasopharyngeal carcinoma analyzed by comparative genomic hybridization.
abstract: Nasopharyngeal carcinoma (NPC) is one of the most common cancers in Malaysia, mainly occurring among the Chinese population. To detect common genetic alterations in NPC, we screened seven cases of NPC using the comparative genomic hybridization (CGH) technique. Before proceeding to the CGH technique, the tumors were first confirmed to consist of 75% tumor cells or more. In brief, the technique consists of binding tumor DNA with normal DNA and human Cot-1 DNA, which is then hybridized to normal metaphase spreads. The slides were then counterstained with 4,6 diamino-2-phenylindole (DAPI II) for detection. Analyses were performed using CGH software (Cytovision). We found genetic alterations in all seven NPC samples. The common chromosomal gains (57%, four cases) were found on chromosome arms 1q, 4p, 5, 7q, 11, 14p, 15q, 18p, and 21p, and common chromosomal losses (43%, three cases) were found on chromosome arm 16p. Our results showed chromosomal alterations in all seven NPC cases in the Malaysian population. This result provides the platform for further investigations to locate tumor suppressor genes and oncogenes at specific chromosomal regions in Malaysian NPC patients.
authors.affiliation: Human Genome Center, Universiti Sains Malaysia, Jalan Raja Perempuan Zainab I, 16150 Kubang Kerian, Kelantan, Malaysia.
authors.forename: M N
authors.initials: MN
authors.lastname: Natasya Naili
authors.forename: C H
authors.initials: CH
authors.lastname: Hasnita
authors.forename: A K
authors.initials: AK
authors.lastname: Shamim
authors.forename: J
authors.initials: J
authors.lastname: Hasnan
authors.forename: M I
authors.initials: MI
authors.lastname: Fauziah
authors.forename: M Y
authors.initials: MY
authors.lastname: Narazah
authors.forename: A
authors.initials: A
authors.lastname: James
authors.forename: S
authors.initials: S
authors.lastname: Zulkiflee
authors.forename: M M T
authors.initials: MM
authors.lastname: Nidzam
authors.forename: B A
authors.initials: BA
authors.lastname: Zilfalil
mesh_terms: D000328:Adult; D000368:Aged; D002277:Carcinoma; D002874:Chromosome Mapping; D002875:Chromosomes / Q000648:ultrastructure*; D055028:Comparative Genomic Hybridization; D004273:DNA, Neoplasm / Q000235:genetics; D005260:Female; D006801:Humans; D008296:Malaysia; D008297:Male; D008875:Middle Aged; D008957:Models, Genetic; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000235:genetics / Q000378:metabolism
chemical_list: D004273:DNA, Neoplasm