title: A novel and effective separation method for single mitochondria analysis.
abstract: To investigate the set of mtDNA molecules contained in small biological structures, powerful techniques for separation are required. We tested flow cytometry (FCM(1)), laser capture microdissection (LCM(2)) and a method using optical tweezers (OT(3)) in combination with a 1μ-Ibidi-Slide with regard to their ability to deposit single mitochondrial particles. The success of separation was determined by real-time quantitative PCR (qPCR(4)) and sequencing analysis. OT revealed the highest potential for the separation and deposition of single mitochondrial particles. The study presents a novel setup for effective separation of single mitochondrial particles, which is crucial for the analysis of single mitochondria.
authors.affiliation: Institute of Legal Medicine, Freiburg University Medical Center, Freiburg, Germany. rene.pflugradt@uniklinik-freiburg.de
authors.forename: René
authors.initials: R
authors.lastname: Pflugradt
authors.forename: Ulrike
authors.initials: U
authors.lastname: Schmidt
authors.forename: Benjamin
authors.initials: B
authors.lastname: Landenberger
authors.forename: Timo
authors.initials: T
authors.lastname: Sänger
authors.forename: Sabine
authors.initials: S
authors.lastname: Lutz-Bonengel
mesh_terms: D000818:Animals; D005434:Flow Cytometry; D008930:Mitochondria, Liver; D016133:Polymerase Chain Reaction; D013552:Swine