title: [The effect of specific immunotherapy on the regulation of Th1/Th2 cell ratio of the patients with allergic rhinitis in serum].
abstract: OBJECTIVE
  To explore the effects of specific immunotherapy by the nasal spray and/or sublingual contain on the regulation the balance of serum Th1/Th2 cell ratio, and the expression of total IgE (tIgE) level in perennial allergic rhinitis patients.
  
  
  METHODS
  Thirty-six cases of allergic rhinitis of perennial (PAR) patients with nasal specific immunotherapy (nasal spray group) were chosen as the object of study, 36 PAR patients with sublingual specific immunotherapy (sublingual group) were chosen for the efficacy comparison group, and 32 cases of healthy adults as control (normal control group). The levels of serum IL-2, IL-4, IL-8 and tIgE in PAR nasal spray group and sublingual group were examined by IRMA and double-antibody sandwich assay before and after the treatment of specific immunotherapy (SIT); The content of IFN-gamma was determined by enzyme-linked immunosorbent assay; The infiltration and changes of eosinophilic granulocyte were observed by the smear of nasal secretions.
  
  
  RESULTS
  In nasal spray group and sublingual group, the contents of serum Th1 cytokines IL-2 and IFN-gamma were reduced levels significantly, while the contents of Th2 cytokines IL-4, IL-8 and tIgE were significantly increased before the treatment of SIT. After the SIT to the maintenance dose, the levels of IL-2 and IFN-gamma in serum were significantly higher than that before treatment, while IL-4, IL-8 and tIgE content were significantly lower in AR patients (P < 0.01 or P < 0.05). The clinical efficacy of nasal spray group and sublingual group were 97.22% and 94.44%, while the two groups was no statistical difference in efficacy (P > 0.05).
  
  
  CONCLUSIONS
  With the presence of Th1/Th2 cell ratio and cytokine imbalance, the AR patients manifested as Th2 cell function in accentuation. Nasal mucosa and/or sublingual in the local SIT can have changed no-balance Th1/Th2 cell of by regulating the balance of serum Th1/Th2 cell ratio, the expression level of cytokine expression and the level of tIgE.
authors.affiliation: Department of Otolaryngology, Affiliated Hospital, Jianghan University, Wuhan 430015, China. xcw626@sina.com
authors.forename: Caiwen
authors.initials: C
authors.lastname: Xiao
authors.forename: Hanlin
authors.initials: H
authors.lastname: Li
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.forename: Zhiying
authors.initials: Z
authors.lastname: Cheng
authors.forename: Jing
authors.initials: J
authors.lastname: Qin
authors.forename: Wei
authors.initials: W
authors.lastname: Zhou
authors.forename: Qingwen
authors.initials: Q
authors.lastname: He
authors.forename: Xiang
authors.initials: X
authors.lastname: Xu
mesh_terms: D000293:Adolescent; D000328:Adult; D016207:Cytokines / Q000097:blood; D005260:Female; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood; D007167:Immunotherapy; D018655:Lymphocyte Count; D008297:Male; D008875:Middle Aged; D012221:Rhinitis, Allergic, Perennial / Q000097:blood* / Q000276:immunology / Q000628:therapy*; D018417:Th1 Cells / Q000166:cytology*; D018418:Th2 Cells / Q000166:cytology*; D055815:Young Adult
chemical_list: D016207:Cytokines; D007073:Immunoglobulin E