title: The Pik-p resistance to Magnaporthe oryzae in rice is mediated by a pair of closely linked CC-NBS-LRR genes.
abstract: The blast resistance gene Pik-p, mapping to the Pik locus on the long arm of rice chromosome 11, was isolated by map-based in silico cloning. Four NBS-LRR genes are present in the target region of cv. Nipponbare, and a presence/absence analysis in the Pik-p carrier cv. K60 excluded two of these as candidates for Pik-p. The other two candidates (KP3 and KP4) were expressed in cv. K60. A loss-of-function experiment by RNAi showed that both KP3 and KP4 are required for Pik-p function, while a gain-of-function experiment by complementation test revealed that neither KP3 nor KP4 on their own can impart resistance, but that resistance was expressed when both were introduced simultaneously. Both Pikp-1 (KP3) and Pikp-2 (KP4) encode coiled-coil NBS-LRR proteins and share, respectively, 95 and 99% peptide identity with the two alleles, Pikm1-TS and Pikm2-TS. The Pikp-1 and Pikp-2 sequences share only limited homology. Their sequence allowed Pik-p to be distinguished from Pik, Pik-s, Pik-m and Pik-h. Both Pikp-1 and Pikp-2 were constitutively expressed in cv. K60 and only marginally induced by blast infection.
authors.affiliation: Laboratory of Plant Resistance and Genetics, College of Resources and Environmental Sciences, South China Agricultural University, Guangzhou, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Yuan
authors.forename: Chun
authors.initials: C
authors.lastname: Zhai
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Wang
authors.forename: Xiaoshan
authors.initials: X
authors.lastname: Zeng
authors.forename: Xiaoke
authors.initials: X
authors.lastname: Xu
authors.forename: Hanqiao
authors.initials: H
authors.lastname: Hu
authors.forename: Fei
authors.initials: F
authors.lastname: Lin
authors.forename: Ling
authors.initials: L
authors.lastname: Wang
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Pan
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D003001:Cloning, Molecular; D018744:DNA, Plant / Q000235:genetics; D017343:Genes, Plant; D005816:Genetic Complementation Test; D018745:Genome, Plant; D007113:Immunity, Innate; D020082:Magnaporthe / Q000472:pathogenicity*; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics* / Q000276:immunology / Q000382:microbiology; D010935:Plant Diseases / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000276:immunology / Q000382:microbiology; D020641:Polymorphism, Single Nucleotide; D034622:RNA Interference; D017422:Sequence Analysis, DNA
chemical_list: D018744:DNA, Plant; D010940:Plant Proteins