title: A new tool in the field of in-vitro diagnosis of allergy: preliminary results in the comparison of ImmunoCAP© 250 with the ImmunoCAP© ISAC.
abstract: BACKGROUND
  The ImmunoCAP© ISAC allows for the determination of specific immunoglobulin E (IgE) against 103 recombinant or purified allergen components in a single analytical step. The aim of our study was to perform a comparison of the specific IgE results measured with the microarray method to those obtained using the traditional method of ImmunoCAP©.
  
  
  METHODS
  We selected 86 clinically relevant patients on the basis of their specific IgE for recombinant allergens (ImmunoCAP© 250, Phadia). Also, we selected two patients with a high total IgE to evaluate the non-specific binding of IgE. All samples were screened with the ImmunoCAP© ISAC. Then, we compared the 555 specific IgE antibodies results provided by ImmunoCAP© ISAC with the specific IgE levels obtained with ImmunoCAP© 250.
  
  
  RESULTS
  We observed that 82 of the 384 results found to be positive with ImmunoCAP© were negative with ISAC© (concordance 78.65%). Of 171 negative results obtained with ImmunoCAP©, 11 were positive with ISAC© (concordance 93.57%). No non-specific binding was observed.
  
  
  CONCLUSIONS
  Our results show that the ImmunoCAP© ISAC has good analytical performance when compared with the ImmunoCAP© 250 method. We did not observe any non-specific binding. However, better sensitivity for some clinically relevant allergen components, such as rPru p 3 is needed.
authors.affiliation: Department of Clinical Chemistry, University Hospital of Liège, Belgium. romy.gadisseur@chu.ulg.ac.be
authors.forename: Romy
authors.initials: R
authors.lastname: Gadisseur
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Chapelle
authors.forename: Etienne
authors.initials: E
authors.lastname: Cavalier
mesh_terms: D000328:Adult; D000818:Animals; D000918:Antibody Specificity; D005260:Female; D006801:Humans; D006967:Hypersensitivity / Q000175:diagnosis* / Q000276:immunology*; D007073:Immunoglobulin E / Q000032:analysis / Q000276:immunology; D008297:Male; D040081:Protein Array Analysis / Q000379:methods*
chemical_list: D007073:Immunoglobulin E