title: [Sphingosine kinase regulates hepatocyte growth factor-induced migration of endothelial cells].
abstract: OBJECTIVE
  To elucidate the effect of sphingosine kinase (SPK) on the hepatocyte growth factor (HGF)-induced migration of endothelial cells.
  
  
  METHODS
  We constructed recombinant adenoviral vectors, which contain SPK gene and its mutant respectively. These adenoviral vectors were packaged and amplified in 293 cells. And intracellular SPK activity was assayed via measurement of [32]P radioisotope labeled S1P; the effect of SPK activation on HGF-induced migration of endothelial cell was observed by Transwell technique.
  
  
  RESULTS
  Adenoviral mediated expression of SPK gene increased in ECV 304 cells intracellular SPK activity, which in turn enhanced the HGF-induced migration. Whereas these activities were blocked by the dominant negative SPK gene.
  
  
  CONCLUSIONS
  These findings show that SPK activation plays important roles in the regulation of HGF-induced migration of endothelial cells.
authors.affiliation: Department of Cardiology, General Hospital, PLA, Beijing 100853, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Yi
authors.forename: Zhuao-Zhuang
authors.initials: ZZ
authors.lastname: Lu
authors.forename: Hai-Feng
authors.initials: HF
authors.lastname: Duan
authors.forename: Lu-Yue
authors.initials: LY
authors.lastname: Gai
authors.forename: Li-Sheng
authors.initials: LS
authors.lastname: Wang
mesh_terms: D000256:Adenoviridae / Q000378:metabolism; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects*; D042783:Endothelial Cells / Q000166:cytology*; D017228:Hepatocyte Growth Factor / Q000494:pharmacology*; D006801:Humans; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D000099157:Sphingosine Kinase
chemical_list: D017228:Hepatocyte Growth Factor; D017853:Phosphotransferases (Alcohol Group Acceptor); D000099157:Sphingosine Kinase