title: [Study on pan-resistant Klebsiella pneumoniae harboring blaKPC-2 type carbapenemase gene from a hospital outbreak in Huzhou, Zhejiang].
abstract: OBJECTIVE
  To investigate the status of genotype of the KPC (Klebsiella pneumoniae carbapenemase)-encoding genes in Pan-resistant K. Pneumoniae, isolated from the 98th Hospital of People's Liberation Army, Huzhou district, Zhejiang province, China.
  
  
  METHODS
  19 strains of Pan-resistant K. pneumoniae were isolated from the inpatients between November, 2008 and July, 2009. Phenotypic confirmatory test for suspected carbapenemases production were carried out by Modified Hodge test. Carbapenemase gene of bla(KPC) was analyzed by PCR and verified by DNA sequencing.
  
  
  RESULTS
  In 19 strains of K. pneumoniae, the positive rates of Modified Hodge test and gene of bla(KPC) were both 100.0%. These genes all belonged to bla(KPC-2) subtype confirmed by nucleotide sequence analysis. Among them, the bla(KPC-2) gene sequence of the HZ001 strain (its original serial number was HZ9871) had been registered in GenBank (GenBank Accession Number: GU086225).
  
  
  CONCLUSIONS
  All of the Pan-resistant K. pneumoniae isolated from the inpatients harbored bla(KPC-2) type carbapenemases gene and causing an outbreak in a hospital. Carbapenemases that producing type KPC-2 might be the major reason which causing the resistance to Carbapenems antibiotics.
authors.affiliation: Microbiology Laboratory, the 98th Hospital of People's Liberation Army, Huzhou 313000, China. hzmccfhzy@163.com
authors.forename: Zhi-mi
authors.initials: ZM
authors.lastname: Huang
authors.forename: Jia-rui
authors.initials: JR
authors.lastname: Mi
authors.forename: Yi-quan
authors.initials: YQ
authors.lastname: Sheng
authors.forename: Yu-xiu
authors.initials: YX
authors.lastname: Zou
authors.forename: Qiu-ju
authors.initials: QJ
authors.lastname: Chu
authors.forename: Li-wei
authors.initials: LW
authors.lastname: Ge
authors.forename: Hai-yan
authors.initials: HY
authors.lastname: Yang
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D002681:China / Q000453:epidemiology; D003428:Cross Infection / Q000382:microbiology*; D004196:Disease Outbreaks; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D005798:Genes, Bacterial; D006801:Humans; D007710:Klebsiella Infections / Q000453:epidemiology / Q000382:microbiology*; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D001618:beta-Lactamases; C531581:carbapenemase-2, Klebsiella pneumoniae