title: Increased expression of tuberin in human uterine leiomyoma.
abstract: Female Eker rats harboring an insertional deletion in one copy of the tuberous sclerosis complex 2 (Tsc2) gene develop uterine leiomyoma, but the underlying mechanism of human uterine leiomyoma is not completely understood. To examine whether down-regulation of tuberin, a TSC2 gene product, is present in human uterine leiomyoma, we analyzed leiomyoma and matched myometrium tissues from 22 Chinese patients with Western blotting and real-time polymerase chain reaction analyses, and found that the expression of tuberin was significantly increased in leiomyoma tissues compared with matched myometrium tissues with inhibition of both the mammalian target of rapacmycin pathway and mitogen-activated protein kinase pathways.
authors.affiliation: Department of Biochemistry and Molecular Biology, Nankai University School of Medicine, Tianjin, People's Republic of China.
authors.forename: Lihua
authors.initials: L
authors.lastname: Cui
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Ren
authors.forename: Hao
authors.initials: H
authors.lastname: Yin
authors.forename: Yuebing
authors.initials: Y
authors.lastname: Wang
authors.forename: Dong
authors.initials: D
authors.lastname: Li
authors.forename: Meng
authors.initials: M
authors.lastname: Liu
authors.forename: Yingjun
authors.initials: Y
authors.lastname: Zhu
authors.forename: Wanjun
authors.initials: W
authors.lastname: Lin
authors.forename: Xiang D
authors.initials: XD
authors.lastname: Tang
authors.forename: Yu
authors.initials: Y
authors.lastname: Gui
authors.forename: Xi-Long
authors.initials: XL
authors.lastname: Zheng
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007889:Leiomyoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D008875:Middle Aged; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism; D009215:Myometrium / Q000378:metabolism* / Q000473:pathology; D058570:TOR Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism; D000077005:Tuberous Sclerosis Complex 2 Protein; D025521:Tumor Suppressor Proteins / Q000235:genetics* / Q000378:metabolism; D015854:Up-Regulation; D014594:Uterine Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology
chemical_list: C000624653:TSC2 protein, human; C000624655:Tsc2 protein, rat; D000077005:Tuberous Sclerosis Complex 2 Protein; D025521:Tumor Suppressor Proteins; C546842:MTOR protein, human; D058570:TOR Serine-Threonine Kinases; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3