title: Expression of mRNA isoforms of latent transforming growth factor-β binding protein-1 in coronary atherosclerosis and human tissues.
abstract: Latent transforming growth factor-β binding protein-1 (LTBP1) has been implicated in the control of secretion, localization, and activation of TGFβ (transforming growth factor-β). We developed a quantitative reverse-transcriptase polymerase chain reaction (Q-RT-PCR) assay using an RNA internal standard to examine the expression of three alternatively spliced isoforms of LTBP1 (LTBP1Δ41, LTBP1Δ53, and LTBP1Δ55) in a variety of human tissues. The assays were also used to determine the expression of LTBP1L and LTBP1S isoforms and total LTBP1. The Q-RT-PCR assays were highly reproducible and showed that in most tissues LTBP1Δ55 and LTBP1L were minor components of LTBP1. The proportion of LTBP1Δ41 ranged from 2% of total LTBP1 mRNA in early coronary atherosclerotic lesions to 54% in advanced lesions.
authors.affiliation: Harvard Medical School, Massachusetts General Hospital, Vascular Imaging and Interventions, 55 Fruit St., Boston, MA 02155, USA. roklu@partners.org.
authors.forename: Rahmi
authors.initials: R
authors.lastname: Oklü
authors.forename: Robin
authors.initials: R
authors.lastname: Hesketh
authors.forename: Stephan
authors.initials: S
authors.lastname: Wicky
authors.forename: James C
authors.initials: JC
authors.lastname: Metcalfe
mesh_terms: D017398:Alternative Splicing; D016022:Case-Control Studies; D003324:Coronary Artery Disease / Q000235:genetics* / Q000378:metabolism; D003331:Coronary Vessels / Q000378:metabolism; D004847:Epithelial Cells / Q000378:metabolism; D005260:Female; D014644:Genetic Variation; D006801:Humans; D053779:Latent TGF-beta Binding Proteins / Q000096:biosynthesis / Q000235:genetics*; D009928:Organ Specificity; D020033:Protein Isoforms; D012333:RNA, Messenger / Q000096:biosynthesis*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: C509032:LTBP1 protein, human; D053779:Latent TGF-beta Binding Proteins; D020033:Protein Isoforms; D012333:RNA, Messenger