title: Loop-mediated isothermal amplification assays for identification of antiseptic- and methicillin-resistant Staphylococcus aureus.
abstract: A method for rapid identification of antiseptic- and methicillin-resistant Staphylococcus aureus (MRSA) based on 3 loop-mediated isothermal amplification (LAMP) assays was developed. LAMP targeting the femB gene identified S. aureus with 100% specificity, and LAMP targeting the mecA gene associated with methicillin resistance identified methicillin-resistant staphylococci with 100% specificity. LAMP targeting the qacA/B gene encoding an efflux pump responsible for antiseptic resistance identified high-acriflavine-resistant (MIC≥100 mg/L) MRSA (92.5% positive) and acriflavine-susceptible (MIC<25 mg/L) MRSA (100% negative). They were performed under the same reaction conditions within 60 min at 63 °C. The combined LAMP assays will be useful for rapid identification of S. aureus isolates and determination of their antibiotic and antiseptic resistance patterns with regard to methicillin and organic cationic substrates.
authors.affiliation: Center for Experimental Animal Research, Iwate Medical University, Morioka, Iwate, Japan.
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Hanaki
authors.forename: Jun-ichiro
authors.initials: J
authors.lastname: Sekiguchi
authors.forename: Kayo
authors.initials: K
authors.lastname: Shimada
authors.forename: Ayako
authors.initials: A
authors.lastname: Sato
authors.forename: Hajime
authors.initials: H
authors.lastname: Watari
authors.forename: Tadashi
authors.initials: T
authors.lastname: Kojima
authors.forename: Tohru
authors.initials: T
authors.lastname: Miyoshi-Akiyama
authors.forename: Teruo
authors.initials: T
authors.lastname: Kirikae
mesh_terms: D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D001431:Bacteriological Techniques / Q000379:methods*; D024881:Drug Resistance, Bacterial; D006801:Humans; D008712:Methicillin / Q000494:pharmacology*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012680:Sensitivity and Specificity; D013210:Staphylococcus; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000302:isolation & purification*; D013696:Temperature
chemical_list: D000891:Anti-Infective Agents, Local; D008712:Methicillin