title: Intracellular calcium level is upregulated by interleukin-32 in auditory cells.
abstract: Interleukin (IL)-32 has been associated with inflammation, apoptosis, and chemokine induction. The intracellular Ca(2+) concentration of mammalian endolymph in the inner ear is required for normal hearing and balance. Here, we document for the first time that IL-32 highly increased intracellular calcium level and IL-1β expression in an auditory cell line, HEI-OC1 cells. Treatment with 1, 2-bis (2-aminophenoxy) ethane-N, N, N', N'-tetraacetic acid acetoxymethyl ester, a chelator of intracellular calcium, inhibited IL-32-induced IL-1 β production and caspase-1 activation. Thus, IL-32 may contribute to modulation of the inflammatory reaction through the regulation of intracellular Ca(2+) in the inner ear.
authors.affiliation: Biochip Research Center, Hoseo University, 165, Sechul-ri, Baebang-myun, Asan, Chungnam 336-795, Republic of Korea.
authors.forename: Hyun-Ja
authors.initials: HJ
authors.lastname: Jeong
authors.forename: Na-Ra
authors.initials: NR
authors.lastname: Han
authors.forename: Phil-Dong
authors.initials: PD
authors.lastname: Moon
authors.forename: Min-Ho
authors.initials: MH
authors.lastname: Kim
authors.forename: Hyung-Min
authors.initials: HM
authors.lastname: Kim
mesh_terms: D002118:Calcium / Q000378:metabolism*; D020170:Caspase 1 / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D006198:Hair Cells, Auditory / Q000187:drug effects* / Q000378:metabolism*; D053583:Interleukin-1beta / Q000096:biosynthesis; D007378:Interleukins / Q000494:pharmacology*; D042541:Intracellular Space / Q000187:drug effects / Q000378:metabolism*; D015854:Up-Regulation / Q000187:drug effects*
chemical_list: D053583:Interleukin-1beta; D007378:Interleukins; D020170:Caspase 1; D002118:Calcium