title: Quantitative analysis of collagen fiber organization in injured tendons using Fourier transform-second harmonic generation imaging.
abstract: Fourier transform-second harmonic generation (FT-SHG) imaging is used as a technique for evaluating collagenase-induced injury in horse tendons. The differences in collagen fiber organization between normal and injured tendon are quantified. Results indicate that the organization of collagen fibers is regularly oriented in normal tendons and randomly organized in injured tendons. This is further supported through the use of additional metrics, in particular, the number of dark (no/minimal signal) and isotropic (no preferred fiber orientation) regions in the images, and the ratio of forward-to-backward second-harmonic intensity. FT-SHG microscopy is also compared with the conventional polarized light microscopy and is shown to be more sensitive to assessing injured tendons than the latter. Moreover, sample preparation artifacts that affect the quantitative evaluation of collagen fiber organization can be circumvented by using FT-SHG microscopy. The technique has potential as an assessment tool for evaluating the impact of various injuries that affect collagen fiber organization.
authors.affiliation: Institute for Genomic Biology, University of Illinois Urbana-Champaign, 1206 West Gregory Drive, Urbana, Illinois 61801, USA.
authors.forename: Mayandi
authors.initials: M
authors.lastname: Sivaguru
authors.forename: Sushmitha
authors.initials: S
authors.lastname: Durgam
authors.forename: Raghu
authors.initials: R
authors.lastname: Ambekar
authors.forename: David
authors.initials: D
authors.lastname: Luedtke
authors.forename: Glenn
authors.initials: G
authors.lastname: Fried
authors.forename: Allison
authors.initials: A
authors.lastname: Stewart
authors.forename: Kimani C
authors.initials: KC
authors.lastname: Toussaint
mesh_terms: D000818:Animals; D024022:Fibrillar Collagens / Q000032:analysis* / Q000737:chemistry*; D005583:Fourier Analysis; D006736:Horses; D008859:Microscopy, Polarization / Q000379:methods*; D013710:Tendons / Q000473:pathology*
chemical_list: D024022:Fibrillar Collagens