title: High frequency of sex chromosomal disomy in spermatozoa of Lebanese infertile men.
abstract: In Lebanon, assisted reproductive techniques (ART) are widely used to overcome infertility, but the genetic risk associated with these techniques is still ignored. In this study, in order to estimate the transmission risk of paternal chromosomal anomalies to ART offspring, the meiotic segregation of chromosomes X, Y, 18, and 21 was analyzed by fluorescent in situ hybridization on the spermatozoa of 19 Lebanese infertile men. Our results show significantly higher frequencies of sex chromosome disomies in the group of patients with oligozoospermia compared with a control group of fertile males. Interestingly, the sex chromosome aneuploidy rates were highly variable between oligozoospermic patients, and ranged between 0.9% and 12.87%. No significant increase in aneuploidy rates was found for the group of nonoligozoospermic patients with asthenozoospermia and/or teratozoospermia. In addition, the disomy rate for chromosome 21 was analyzed in 8 patients, in whom higher disomy rates were shown as compared with the controls. Altogether, the results suggest that Lebanese oligozoospermic men undergoing ART may have an increased risk of transmitting sex chromosome anomalies to their offspring, as well as, in some cases, trisomy 21. Based on this work, genetic counseling programs for Lebanese infertile couples undergoing ART procedures should be developed, in order to improve the investigation and selection of Lebanese infertile couple candidates for ART procedures and optimize the choice of ART techniques.
authors.affiliation: Faculty of Sciences II, Lebanese University, Fanar, Matn BP 90656, Lebanon. hazzourim@yahoo.com
authors.forename: Berthes G
authors.initials: BG
authors.lastname: Younes
authors.forename: Khaled M
authors.initials: KM
authors.lastname: Hazzouri
authors.forename: Mustapha J
authors.initials: MJ
authors.lastname: Chaaban
authors.forename: Walid G
authors.initials: WG
authors.lastname: Karam
authors.forename: Imad F
authors.initials: IF
authors.lastname: Abou Jaoude
authors.forename: Joanna H
authors.initials: JH
authors.lastname: Attieh
authors.forename: Mira M
authors.initials: MM
authors.lastname: Hazzouri
mesh_terms: D000328:Adult; D000782:Aneuploidy; D002891:Chromosomes, Human, Pair 21; D041322:Chromosomes, Human, Y; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007248:Infertility, Male / Q000235:genetics*; D007861:Lebanon; D008297:Male; D027724:Reproductive Techniques, Assisted; D013094:Spermatozoa