title: Nanostructural materials increase mineralization in bone cells and affect gene expression through miRNA regulation.
abstract: We report that several nanomaterials induced enhanced mineralization (increased numbers and larger areas of mineral nests) in MC3T3-E1 bone cells, with the highest response being induced by silver nanoparticles (AgNPs). We demonstrate that AgNPs altered microRNA expression resulting in specific gene expression associated with bone formation. We suggest that the identified essential transcriptional factors and bone morphogenetic proteins play an important role in activation of the process of mineralization in bone cells exposed to AgNPs.
authors.affiliation: University of Arkansas at Little Rock, Nanotechnology Center, Applied Science Department, Little Rock, AR 72204, USA.
authors.forename: Meena
authors.initials: M
authors.lastname: Mahmood
authors.forename: Zhiguang
authors.initials: Z
authors.lastname: Li
authors.forename: Daniel
authors.initials: D
authors.lastname: Casciano
authors.forename: Mariya V
authors.initials: MV
authors.lastname: Khodakovskaya
authors.forename: Tao
authors.initials: T
authors.lastname: Chen
authors.forename: Alokita
authors.initials: A
authors.lastname: Karmakar
authors.forename: Enkeleda
authors.initials: E
authors.lastname: Dervishi
authors.forename: Yang
authors.initials: Y
authors.lastname: Xu
authors.forename: Thikra
authors.initials: T
authors.lastname: Mustafa
authors.forename: Fumiya
authors.initials: F
authors.lastname: Watanabe
authors.forename: Ashley
authors.initials: A
authors.lastname: Fejleh
authors.forename: Morgan
authors.initials: M
authors.lastname: Whitlow
authors.forename: Mustafa
authors.initials: M
authors.lastname: Al-Adami
authors.forename: Anindya
authors.initials: A
authors.lastname: Ghosh
authors.forename: Alexandru S
authors.initials: AS
authors.lastname: Biris
mesh_terms: D016475:3T3 Cells; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D019485:Bone Morphogenetic Proteins / Q000378:metabolism*; D001842:Bone and Bones / Q000166:cytology; D002113:Calcification, Physiologic / Q000187:drug effects / Q000235:genetics; D002460:Cell Line; D015870:Gene Expression; D053768:Metal Nanoparticles; D051379:Mice; D035683:MicroRNAs / Q000378:metabolism; D049329:Nanostructures; D010006:Osteoblasts / Q000378:metabolism*; D010012:Osteogenesis; D012834:Silver
chemical_list: D019485:Bone Morphogenetic Proteins; D035683:MicroRNAs; D012834:Silver; D000469:Alkaline Phosphatase