title: Novel steroidal saponins from Liriope graminifolia (Linn.) Baker with anti-tumor activities.
abstract: Phytochemical investigation of the underground parts of Liriope graminifolia (Linn.) Baker resulted in the isolation of two new steroidal saponins lirigramosides A (1) and B (2) along with four known compounds. The structures were determined by extensive spectral analysis, including two-dimensional (2D) NMR spectroscopy and chemical methods, to be 3-O-[β-d-xylopyranosyl-(1→3)-α-l-arabinopyranosyl-(1→2)-[α-l-rhamnopyranosyl-(1→4)]-β-d-glucopyranosyl-(25S)-spirost-5-ene-3β,17α-diol (1), 1-O-[α-l-rhamnopyranosyl-(1→2)-β-d-xylopyranosyl]-(25R)-ruscogenin (2), 1-O-β-d-xylopyranosyl-3-O-α-l-rhamnopyranosyl-(25S)-ruscogenin (3), 3-O-α-l-rhamnopyranosyl-1-O-sulfo-(25S)-ruscogenin (4), methylophiopogonanone B (5), and 5,7-dihydroxy-3-(4-methoxybenzyl)-6-methyl-chroman-4-one, (ophiopogonanone B, 6), respectively. Compound 1 has a new (25S)-spirost-5-ene-3β,17α-diol ((25S)-pennogenin) aglycone moiety. The isolated compounds were evaluated for their cytotoxic activities against Hela and SMMC-7721 cells.
authors.affiliation: School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310035, PR China. wkw220@yahoo.com.cn
authors.forename: Kui-Wu
authors.initials: KW
authors.lastname: Wang
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.forename: Lian-Qing
authors.initials: LQ
authors.lastname: Shen
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D002240:Carbohydrate Sequence; D006367:HeLa Cells; D006801:Humans; D019668:Liliaceae / Q000737:chemistry*; D008969:Molecular Sequence Data; D012503:Saponins / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D013256:Steroids / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: C557788:1-O-(rhamnopyranosyl-(1-2)-xylopyranosyl)ruscogenin; C557787:3-O-(xylopyranosyl-(1-3)-arabinopyranosyl-(1-2)-(rhamnopyranosyl-(1-4)-glucopyranosyl))spirost-5-ene-3,17-diol; D000970:Antineoplastic Agents; D012503:Saponins; D013256:Steroids