title: [A fluorospectrophotometric determination of nitrite in blood].
abstract: OBJECTIVE
  To establish a fluorospectrophotometric assay for the measurement of nitrite in blood.
  
  
  METHODS
  Interference from hemoglobin and other blood ingredients was removed through sulfuric acid and phosphotungstic acid pretreatment. Fluorescence of 1-[H]-naphthotriazole from the reaction of 2,3-diaminonaphthalene with nitrite was determined with fluorospectrophotometry.
  
  
  RESULTS
  The following conditions were proper: Serum or plasma was treated with sulfuric acid and phosphotungstic acid pretreatment for two times, 2,3-diaminonaphthalene of 0.63 mmol x (L(-1)) was used, reaction solution pH and final pH were about 1.60 and 1.70 respectively, solution containing 2,3-diaminonaphthalene and supernatant after pretreatment was water-bathed at 20 degrees C for 15 minutes. The lower limit of detection was 24.27 nmol x L(-1). Nitrite determined in peripheral blood of healthy people was (10.91 +/- 2.38) micromol x L(-1), and its 95% distribution range was (6.24-15.57) micromol x L(-1).
  
  
  CONCLUSIONS
  It's a relatively sensitive, specific, simple method. It's of some value to the study of nitric oxide.
authors.affiliation: Department of Pharmacology, Tianjin Medical University, Tianjin 300070, China.
authors.forename: Bu-wu
authors.initials: BW
authors.lastname: Fang
authors.forename: Zhu-hua
authors.initials: ZH
authors.lastname: Jin
authors.forename: Xiu-zhen
authors.initials: XZ
authors.lastname: Lin
mesh_terms: D015590:Fluorophotometry; D006801:Humans; D057230:Limit of Detection; D009573:Nitrites / Q000097:blood*
chemical_list: D009573:Nitrites