title: Selective pretreatment and determination of phenazopyridine using an imprinted polymer-electrospray ionization ion mobility spectrometry system.
abstract: In this research, selective separation and determination of phenazopyridine (PAP) is demonstrated using molecular imprinted polymer (MIP) coupled with electrospray ionization ion mobility spectrometry (ESI-IMS). In the non-covalent approach, selective MIP produced using PAP and methacrylic acid (MAA) as a template molecule and monomer, respectively. The created polymer is utilized as a media for solid-phase extraction (SPE), revealing selective binding properties for the analyte from pharmaceutical and serum samples. A coupled MIP-IMS makes it possible to quantitize PAP in the range of 1-100 ng mL(-1) and with a 0.2 ng mL(-1) detection limit. Furthermore, the MIP selectivity is evaluated by application of some substances with analogous and different molecular structures to that of PAP. This method is successfully applied for the determination of PAP in pharmaceutical and serum samples.
authors.affiliation: Department of Chemistry, Isfahan University of Technology, Isfahan 84156-83111 Islamic Republic of Iran. rezaei@cc.iut.ac.ir
authors.forename: B
authors.initials: B
authors.lastname: Rezaei
authors.forename: M T
authors.initials: MT
authors.lastname: Jafari
authors.forename: O
authors.initials: O
authors.lastname: Rahmanian
mesh_terms: D053000:Analytic Sample Preparation Methods / Q000379:methods*; D002138:Calibration; D006801:Humans; D006863:Hydrogen-Ion Concentration; D054802:Molecular Imprinting; D004364:Pharmaceutical Preparations / Q000737:chemistry; D010621:Phenazopyridine / Q000032:analysis* / Q000097:blood / Q000302:isolation & purification*; D011108:Polymers / Q000138:chemical synthesis*; D012997:Solvents / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013997:Time Factors
chemical_list: D004364:Pharmaceutical Preparations; D011108:Polymers; D012997:Solvents; D010621:Phenazopyridine