title: Production and characterisation of monoclonal antibodies specific for chicken interleukin-12.
abstract: Using genetic immunisation of mice, we produced antibodies against chicken interleukin-12p40 (chIL-12p40), also known as IL-12β. After a final injection with a recombinant chIL-12p40 protein, several stable hybridoma cell lines were established which secreted monoclonal antibodies (mAbs) to this component of the heterodimeric IL-12 cytokine. Specific binding of three of the mAbs to COS-7 cell-derived recombinant chIL-12p40 and the chIL-12p70 heterodimer was demonstrated in an indirect ELISA, and in dot blots. Two of the mAbs were used to develop a capture ELISA, suitable for detecting both recombinant protein (chIL-12p40 and the heterodimeric p70 protein) and native chIL-12. The mAbs were further characterised to show utility in immunocytochemistry.
authors.affiliation: Institute for Animal Health, Compton, Berkshire RG20 7NN, UK.
authors.forename: S
authors.initials: S
authors.lastname: Balu
authors.forename: L
authors.initials: L
authors.lastname: Rothwell
authors.forename: P
authors.initials: P
authors.lastname: Kaiser
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D002645:Chickens / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D015870:Gene Expression / Q000276:immunology; D006825:Hybridomas; D066298:In Vitro Techniques; D018664:Interleukin-12 / Q000032:analysis / Q000235:genetics / Q000276:immunology; D053762:Interleukin-12 Subunit p40 / Q000032:analysis* / Q000235:genetics / Q000276:immunology*; D008264:Macrophages / Q000276:immunology; D051379:Mice; D011994:Recombinant Proteins / Q000032:analysis / Q000276:immunology; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary
chemical_list: D000911:Antibodies, Monoclonal; D053762:Interleukin-12 Subunit p40; D011994:Recombinant Proteins; D018664:Interleukin-12