title: Preparation and characterization of magnetic chitosan microsphere sorbent for separation and determination of environmental estrogens through SPE coupled with HPLC.
abstract: In this paper, a new separation sorbent of magnetic chitosan microsphere was prepared by means of suspension process using magnetic-Fe(3)O(4) nanoparticles as magnetic material and glutaraldehyde as the cross-linker. The morphology and properties of magnetic chitosan microspheres were characterized by FT-IR, SEM and adsorption test, and the results showed that they had good recognition, high selective ability and fast adsorption-desorption dynamics for estriol (E3), 17β-estradiol (E2) and diethylstilbestrol (DES). Applying this material as SPE sorbent, a method for separation, enrichment and analysis of estrogens in samples coupled with HPLC was developed. Under the optimized condition, the detection limit (S/N=3) for three estrogens was 3.2-20.1 ng/L, and the RSD for five replicate extractions of 50 μg/L E3, E2 and DES was 4.6-8.3%. The blank river water spiked with E3, E2 and DES at 0.05 and 0.2 mg/L levels were extracted and determined by the developed method with good recoveries ranging from 85.5 to 103.7%.
authors.affiliation: College of Food Science and Engineering, Shandong Agricultural University, Taian, P. R. China.
authors.forename: Zhixiang
authors.initials: Z
authors.lastname: Xu
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Li
authors.initials: L
authors.lastname: Cong
authors.forename: Ling
authors.initials: L
authors.lastname: Meng
authors.forename: Jiaming
authors.initials: J
authors.lastname: Song
authors.forename: Jie
authors.initials: J
authors.lastname: Zhou
authors.forename: Xuguang
authors.initials: X
authors.lastname: Qiao
mesh_terms: D048271:Chitosan / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D004967:Estrogens / Q000032:analysis*; D006801:Humans; D008280:Magnetics; D053768:Metal Nanoparticles / Q000737:chemistry; D008863:Microspheres; D015394:Molecular Structure; D052616:Solid Phase Extraction / Q000295:instrumentation / Q000379:methods*; D017550:Spectroscopy, Fourier Transform Infrared; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D004967:Estrogens; D014874:Water Pollutants, Chemical; D048271:Chitosan