title: Highly selective oxidation of benzyl alcohol using engineered Gluconobacter oxydans in biphasic system.
abstract: The Gluconobacter oxydans M5 with disruption of the pyrroloquinoline quinine-dependent membrane-bound aldehyde dehydrogenase (ALDH) was used for the oxidation of benzyl alcohol. The selectivity toward benzaldehyde showed an obvious increase for the engineered strain, which reached the 67.3%, while the wild strain had only 2.8%. Meantime, the aqueous/isooctane (1:1) biphasic system was used for the further improvement of selectivity. By these methods, nearly 100% selectivity and conversion rate could be obtained within 1 h at the optimum initial benzyl alcohol concentration of 5.0 g/l.
authors.affiliation: New World Institute of Biotechnology, State Key Lab of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Wu
authors.forename: Ming Hua
authors.initials: MH
authors.lastname: Li
authors.forename: Jin Ping
authors.initials: JP
authors.lastname: Lin
authors.forename: Dong Zhi
authors.initials: DZ
authors.lastname: Wei
mesh_terms: D000444:Aldehyde Dehydrogenase / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D019905:Benzyl Alcohol / Q000378:metabolism*; D001711:Biotransformation; D005818:Genetic Engineering; D020600:Gluconobacter oxydans / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism*; D010084:Oxidation-Reduction
chemical_list: D001426:Bacterial Proteins; D000444:Aldehyde Dehydrogenase; D019905:Benzyl Alcohol