title: [Testosterone has beneficial effects on human umbilical vein endothelial cells].
abstract: OBJECTIVE
  To investigate the influences of testosterone with varied concentrations on the functions of HUVEC.
  
  
  METHODS
  Human umbilical vein endothelial cells within 2-3 passages were cultured with testosterone (3 x 10(-10) to 3 x 10(-8), 3 x 10(-6), 3 x 10(-5) mol/ L), and the control confluent cells were cultured in the same medium without steroid. MTT experiment was repeated for 7 days to investigate each groups' cell proliferation. The values of NO were tested as recommended. The tPA and PAI-1 antigen levels were assayed with ELISA Kits.
  
  
  RESULTS
  Testosterone at physiologic or lower concentrations (3 x 10(-10) to 3 x 10(-8) mol/L ) had no adverse effect on A490 and NO level, meanwhile, stimulated the secretion of tPA (P < 0.01). However, tPA levels markedly reduced at larger dose (3 x 10(-6) to 3 x 10(-5) mol/L). On the other hand, PAI-1 antigen levels decreased significantly at the testosterone concentrations ranging from 3 x 10(-10) to 3 x 10(-5) mol/L (P < 0.05).
  
  
  CONCLUSIONS
  Testosterone at physiologically relevant concentrations affectively decreased PAI-1, while increased tPA levels, which suggested that testosterone might have beneficial effects on the Human umbilical vein endothelial cells and cardiovascular system to prevent atherosclerosis.
authors.affiliation: Cardiology Department of the First Affiliated Hospital of Harbin Medical University Harbin 150001, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Jin
authors.forename: Lu
authors.initials: L
authors.lastname: Fu
authors.forename: Yi-Fang
authors.initials: YF
authors.lastname: Mei
authors.forename: Li-Jun
authors.initials: LJ
authors.lastname: Zhou
authors.forename: Qian-Ping
authors.initials: QP
authors.lastname: Gao
authors.forename: Jia
authors.initials: J
authors.lastname: Li
mesh_terms: D002478:Cells, Cultured; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects* / Q000378:metabolism*; D006801:Humans; D009569:Nitric Oxide / Q000378:metabolism; D017395:Plasminogen Activator Inhibitor 1 / Q000378:metabolism; D013739:Testosterone / Q000494:pharmacology*; D010959:Tissue Plasminogen Activator / Q000378:metabolism
chemical_list: D017395:Plasminogen Activator Inhibitor 1; D009569:Nitric Oxide; D013739:Testosterone; D010959:Tissue Plasminogen Activator