title: [The expression and control of telomerase in preantral ovary].
abstract: OBJECTIVE
  To study the expression and control of telomerase in rat preantral ovary.
  
  
  METHODS
  Added different factors to the preantral ovarian granulosa cells, then using TRAP-ELISA to analyze the expression of telomerase.
  
  
  RESULTS
  Telomerase activation was detected in granulosa cells. Telomerase activation could be significantly enhanced by hcG, FSH, verapamil and dbcAMP, and it was significantly reduced by antisense-c-myb ODN treatment. We also used radioimmunoassay to determine proterone and estrogen in these cell culture mediums. It was found that these two hormones' secretion were raised under verapamil and FSH; no change under hcG and dbcAMllted with telomerase activity. In MTT assay, the antisense hTERT ODN could significantly inhibited the proliferation of ovarian granulosa cells.
  
  
  CONCLUSIONS
  It is suggested that telomerase activation is present in ovary antral granulosa cell. Anti-c-myb might inhibit telomerase activation, while FSH, hcG, verapamil, dbcAMP might enhance the telomerase activation.
authors.affiliation: The Assistance Reproduction Branch, First Affiliated Hospital of Nanchang University, Nanchang 330006, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Yue-Hui
authors.initials: YH
authors.lastname: Zheng
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Zheng
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D004958:Estradiol / Q000502:physiology; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D006107:Granulosa Cells / Q000166:cytology / Q000201:enzymology*; D010053:Ovary / Q000166:cytology* / Q000201:enzymology; D051381:Rats; D017207:Rats, Sprague-Dawley; D019098:Telomerase / Q000378:metabolism / Q000502:physiology*; D014700:Verapamil / Q000494:pharmacology
chemical_list: D006063:Chorionic Gonadotropin; D004958:Estradiol; D005640:Follicle Stimulating Hormone; D014700:Verapamil; D019098:Telomerase