title: Preparation and application of organic-silica hybrid monolithic capillary columns.
abstract: Organic-silica hybrid monolithic columns have drawn more and more attention due to the ease of preparation and good mechanical stability in recent years. Many synthetic approaches have been developed and a variety of hybrid monolithic capillary columns have been prepared. The sol-gel process is well recognized in the fabrication of hybrid monolithic columns, which can be mainly classified as one-step, acid/base two-step procedures. The new approaches such as the "one-pot" and nano-scaled inorganic-organic hybrid reagent of polyhedral oligomeric silsesquioxane used as a cross-linker have also emerged for the preparation of hybrid monolithic columns. The applications of the organic-silica hybrid monolithic capillary columns for capillary electrochromatography, micro high-performance liquid chromatography, solid-phase micro-extraction and enzymatic reactor etc. are included in this review.
authors.affiliation: CAS Key Laboratory of Separation Sciences for Analytical Chemistry, National Chromatographic R & A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, P. R. China.
authors.forename: Minghuo
authors.initials: M
authors.lastname: Wu
authors.forename: Ren'an
authors.initials: R
authors.lastname: Wu
authors.forename: Zhenbin
authors.initials: Z
authors.lastname: Zhang
authors.forename: Hanfa
authors.initials: H
authors.lastname: Zou
mesh_terms: D019149:Bioreactors; D002851:Chromatography, High Pressure Liquid; D019075:Electrophoresis, Capillary / Q000379:methods*; D004798:Enzymes / Q000737:chemistry; D017646:Organosilicon Compounds / Q000138:chemical synthesis*; D012822:Silicon Dioxide / Q000737:chemistry*; D052617:Solid Phase Microextraction
chemical_list: D004798:Enzymes; D017646:Organosilicon Compounds; D012822:Silicon Dioxide