title: The hypothesis of the main role of H2S in coupled sulphide-nitroso signalling pathway.
abstract: As a part of the nitroso signalling pathway, nitroso-compounds serve as stores and carriers of NO; as part of the sulphide signalling pathway, bound sulfane-sulphur compounds serve as stores and carriers of H2S. Here we hypothesise a coupled sulphide-nitroso signalling pathway, in which H2S plays a main role. H2S releases NO from the endogenous S-nitroso-compounds nitroso-cysteine, nitroso-acetylcysteine and nitroso-albumin. Relaxation of noradrenaline-precontracted aortic rings by H2S is also enhanced in the presence of nitroso-albumin, which may implicate the involvement of the nitroso signalling pathway. Pretreatment of albumin, cysteine, N-acetylcysteine and lipids with H2S results in binding of sulphur to these compounds creating thus new-modified sulphur compounds that release NO from nitroso-compounds directly and/or through released H2S, which suggests sulphide-nitroso signalling pathway participation. This hypothesis is supported by the observation that the pretreatment of noradrenaline-precontracted aortic rings with H2S significantly enhanced relaxation induced by nitroso-glutathione in the absence of H2S. We assume that the NO release from nitroso-compounds directly by H2S or indirectly by the H2S-induced sulphur-bound compounds represents coupled sulphide-nitroso signalling, which may explain some of the numerous biological effects of H2S that are shared with NO.
authors.affiliation: Institute of Molecular Physiology and Genetics, Slovak Academy of Sciences, Vlarska 5, 833 34 Bratislava, Slovakia.
authors.forename: Anna
authors.initials: A
authors.lastname: Bertova
authors.forename: Sona
authors.initials: S
authors.lastname: Cacanyiova
authors.forename: Frantisek
authors.initials: F
authors.lastname: Kristek
authors.forename: Olga
authors.initials: O
authors.lastname: Krizanova
authors.forename: Karol
authors.initials: K
authors.lastname: Ondrias
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Tomaskova
mesh_terms: D000818:Animals; D001011:Aorta / Q000187:drug effects / Q000378:metabolism; D002417:Cattle; D005978:Glutathione / Q000494:pharmacology; D008297:Male; D009589:Nitrogen Oxides / Q000378:metabolism; D009603:Nitroso Compounds / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012710:Serum Albumin, Bovine / Q000494:pharmacology; D015398:Signal Transduction / Q000187:drug effects; D013440:Sulfides / Q000378:metabolism* / Q000494:pharmacology; D013997:Time Factors
chemical_list: D009589:Nitrogen Oxides; D009603:Nitroso Compounds; D013440:Sulfides; D012710:Serum Albumin, Bovine; C025451:sodium bisulfide; D005978:Glutathione