title: [The study of transfected cardiomyocytes by recombinant adenovirus and adeno-associated virus].
abstract: OBJECTIVE
  Recombinant adenovirus (rAd) and adeno-associated virus (rAAV) were created, in which beta2-adrenergic receptors (beta2-AR) gene is under control of the cmv promotor, the cultured neonate rat ventricular myocytes were infected by the two vectors, and the expression of beta2-AR on cultured neonate rat ventricular myocytes was assessed.
  
  
  RESULTS
  RT-PCR demonstrated the presence of beta2-AR mRNA, protein immunoblots demonstrated the expression of the beta2-AR gene. According to a ligand binding assay, the density of beta AR in the cardiomyocytes infected by rAd and rAAV had no difference, which was greater than that in the control.
  
  
  CONCLUSIONS
  The results above demonstrated that adenovirus vector and AAV vector transfected efficiently cardiomyocytes.
authors.affiliation: Department of Cardiology General Hospital of PLA, Beijing 100853, China.
authors.forename: W Q
authors.initials: WQ
authors.lastname: Gao
authors.forename: X Y
authors.initials: XY
authors.lastname: Li
authors.forename: X B
authors.initials: XB
authors.lastname: Wu
authors.forename: X T
authors.initials: XT
authors.lastname: Pei
authors.forename: L
authors.initials: L
authors.lastname: Li
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D002460:Cell Line; D000229:Dependovirus / Q000235:genetics*; D005822:Genetic Vectors; D006801:Humans; D032383:Myocytes, Cardiac / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics; D051381:Rats; D014162:Transfection