title: Two novel antimicrobial peptides from skin secretions of the frog, Rana nigrovittata.
abstract: Two novel antimicrobial peptides with similarity to brevinin-2 family are purified and characterized from the skin secretions of the frog, Rana nigrovittata. Their amino acid sequences were determined as GAFGNFLKGVAKKAGLKILSIAQCKLSGTC (brevinin-2-RN1) and GAFGNFLKGVAKKAGLKILSIAQCKLFGTC (brevinin-2-RN2), respectively, by Edman degradation. Different from brevinin-2, which is composed of 33 amino acid residues (aa), both brevinin-2-RN1 and -RN2 contain 30 aa. Five cDNA sequences (Genbank accession numbers, EU136465-9) encoding precursors of brevinin-2-RN1 and -RN2 were screened from the skin cDNA library of R. nigrovittata. These precursors are composed of 72 aa including a predicted signal peptide, an acidic spacer peptide, and a mature brevinin-2-RN. Both brevinin-2-RN1 and -RN2 showed strong antimicrobial activities against gram-positive and gram-negative bacteria and fungi. The current work identified and characterized two novel antimicrobial peptides with unique primary structure.
authors.affiliation: Life Sciences College of Nanjing Agricultural University, Nanjing, Jiangsu 210095, China.
authors.forename: Xiuhong
authors.initials: X
authors.lastname: Liu
authors.forename: Rui
authors.initials: R
authors.lastname: Liu
authors.forename: Lin
authors.initials: L
authors.lastname: Wei
authors.forename: Hailong
authors.initials: H
authors.lastname: Yang
authors.forename: Keyun
authors.initials: K
authors.lastname: Zhang
authors.forename: Jingze
authors.initials: J
authors.lastname: Liu
authors.forename: Ren
authors.initials: R
authors.lastname: Lai
mesh_terms: D000595:Amino Acid Sequence; D029845:Amphibian Proteins / Q000235:genetics / Q000494:pharmacology*; D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology; D023181:Antimicrobial Cationic Peptides / Q000235:genetics / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects*; D001483:Base Sequence; D005658:Fungi / Q000187:drug effects*; D008969:Molecular Sequence Data; D010455:Peptides / Q000235:genetics / Q000494:pharmacology*; D011898:Ranidae / Q000502:physiology*; D012867:Skin / Q000378:metabolism*
chemical_list: D029845:Amphibian Proteins; D000890:Anti-Infective Agents; D023181:Antimicrobial Cationic Peptides; D010455:Peptides; C078414:brevinin-2, Rana