title: Investigation of localisation of Leptospira spp. in uterine and fetal tissues of non-pregnant and pregnant farmed deer.
abstract: OBJECTIVE
  To find evidence for localisation in the uterus, and fetal infection, of Leptospira spp. in farmed deer in the lower North Island of New Zealand during and shortly after the breeding season.
  
  
  METHODS
  Between February and July 2008, 116 blood samples, 120 kidneys, 120 uteri and 27 fetuses were collected from 120 mixed-age hinds from lines from nine farms, at a deer slaughter premises. Serum samples were tested for antibodies against Leptospira borgpetersenii serovar Hardjo-bovis and Leptospira interrogans serovar Pomona, using the microscopic agglutination test (MAT). For both serovars, a titre of ≥1:48 was considered positive. Samples from kidneys, uteri and fetal tissue were subjected to bacterial culture, using Ellinghausen-McCullough-Johnson-Harris (EMJH) medium, and real-time PCR, using DNA gyrase subunit B gene primers.
  
  
  RESULTS
  Thirty-four of 116 (29.3%) serum samples were positive for serovar Hardjo-bovis, and 13 (11.2%) for serovar Pomona. Seven of 120 kidneys were positive for serovar Hardjo-bovis by culture, and five of these, but no others, were positive by real-time PCR. Of 120 uteri, none was culture- or PCR-positive. None of 27 fetal samples was culture-positive but one was positive by real-time PCR. The dam of the PCR-positive fetus was culture-negative from the kidney, but had an MAT titre of 1:192 for Hardjo-bovis.
  
  
  CONCLUSIONS
  Attempts to isolate Leptospira spp. from the genital tracts and early fetuses of farmed deer were unsuccessful. However, molecular evidence suggested fetal infection in one case. This finding justifies further study of the role of leptospires in the genital tract and fetus and its association with reproductive loss in farmed deer.
authors.affiliation: Institute of Veterinary, Animal and Biomedical Sciences, Massey University, Palmerston North 4442, New Zealand. art.subharat@agresearch.co.nz
authors.forename: S
authors.initials: S
authors.lastname: Subharat
authors.forename: P R
authors.initials: PR
authors.lastname: Wilson
authors.forename: C
authors.initials: C
authors.lastname: Heuer
authors.forename: J M
authors.initials: JM
authors.lastname: Collins-Emerson
mesh_terms: D037881:Aborted Fetus / Q000382:microbiology; D000818:Animals; D003670:Deer; D005260:Female; D007668:Kidney / Q000382:microbiology; D007919:Leptospira / Q000302:isolation & purification*; D007922:Leptospirosis / Q000382:microbiology / Q000662:veterinary*; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000382:microbiology / Q000662:veterinary*; D014599:Uterus / Q000382:microbiology*