title: Cellular response of RAW 264.7 to spray-coated multi-walled carbon nanotube films with various surfactants.
abstract: The increasing role of carbon nanotubes (CNTs) in various biological applications has led to a number of studies on the cytotoxicity of solution-phase CNTs, but few studies are available concerning the cytotoxicity of CNT films. Herein, we studied the potential health effect of CNT films fabricated with three commercial surfactants (sodium cholate, sodium dodecyl sulfate, and triton X-100). Multi-walled carbon nanotube-surfactant dispersions were coated onto substrates through air-spray technique. Cellular morphology, MTT assays, as well as the expression of TNF-α and IL-1β of RAW 264.7 cells cultured on the spray-coated CNT films were evaluated for cytotoxicity. It was found that the cytotoxicity of the CNT films was largely dependent on the type of surfactant used and could be significantly reduced by mild washing steps.
authors.affiliation: School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore, 637457.
authors.forename: Hailan
authors.initials: H
authors.lastname: Chen
authors.forename: Jianmin
authors.initials: J
authors.lastname: Shen
authors.forename: Guo
authors.initials: G
authors.lastname: Longhua
authors.forename: Yuan
authors.initials: Y
authors.lastname: Chen
authors.forename: Dong-Hwan
authors.initials: DH
authors.lastname: Kim
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000494:pharmacology*; D053583:Interleukin-1beta / Q000378:metabolism; D008264:Macrophages / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D051379:Mice; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure; D013499:Surface Properties / Q000187:drug effects; D013501:Surface-Active Agents / Q000494:pharmacology*; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D020099:Coated Materials, Biocompatible; D053583:Interleukin-1beta; D037742:Nanotubes, Carbon; D013501:Surface-Active Agents; D014409:Tumor Necrosis Factor-alpha