title: 17 beta-estradiol attenuates pressure overload-induced myocardial hypertrophy through regulating caveolin-3 protein in ovariectomized female rats.
abstract: Our findings indicate that in ovariectomized female rats abdominal aortic constriction led to significant increases in left ventricular mass, myocyte diameter and heart weight/body weight (HW/BW) value, and decreases in interventricular septal thickness at diastole (IVSd), left ventricular percent fractional shortening (FS) and ejection fraction (EF). These pathophysiological alterations were largely reversed by administration with 17β-estradiol for eight weeks. Furthermore, the enhanced expression of extracellular signal-regulated kinases 1/2 and decreased expression of caveolin-3 were found in left ventricle of AAC group. 17β-estradiol (E(2)) administration increased the expression of caveolin-3 and reduced the level of ERK phosphorylation in these pressure-overloaded rats. Moreover, in cultured neonatal rat cardiomyocytes, E(2) inhibited the hypertrophic response to angiotensin II. This effect was reinforced by the addition of extracellular signal-regulated kinases 1/2 inhibitor PD98059, but was impaired when the cells were pretreated with caveolae disruptor, methyl-β-cyclodextrin (M-β-CD). In conclusion, our data indicate that estrogen attenuates the hypertrophic response induced by pressure overload through down-regulation of extracellular signal-regulated kinases 1/2 phosphorylation and up-regulation of caveolin-3 expression.
authors.affiliation: Department of Physiology, Zhongshan School of Medicine, Sun Yat-Sen University, 74 Zhongshan Rd 2, Guangzhou 510080, People's Republic of China.
authors.forename: Yu-Hong
authors.initials: YH
authors.lastname: Cui
authors.forename: Zhi
authors.initials: Z
authors.lastname: Tan
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Fu
authors.forename: Qiu-Ling
authors.initials: QL
authors.lastname: Xiang
authors.forename: Jin-Wen
authors.initials: JW
authors.lastname: Xu
authors.forename: Ting-Huai
authors.initials: TH
authors.lastname: Wang
mesh_terms: D000818:Animals; D015153:Blotting, Western; D021941:Caveolae / Q000187:drug effects / Q000378:metabolism; D051244:Caveolin 3 / Q000378:metabolism*; D004562:Electrocardiography; D004958:Estradiol / Q000494:pharmacology*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D005260:Female; D006984:Hypertrophy; D009206:Myocardium / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology*; D010052:Ovariectomy; D011312:Pressure; D051381:Rats; D017207:Rats, Sprague-Dawley; D047392:beta-Cyclodextrins / Q000494:pharmacology
chemical_list: D051244:Caveolin 3; D047392:beta-Cyclodextrins; C108732:methyl-beta-cyclodextrin; D004958:Estradiol; D048049:Extracellular Signal-Regulated MAP Kinases