title: [Effect of lipopolysaccharides extracted from Porphyromonas endodontalis on the expression of CD14 in osteoblast].
abstract: OBJECTIVE
  To investigate the effect of lipopolysaccharides(LPS) extracted from Porphyromonas endodontalis(P.e) on the expression of CD14 in osteoblast.
  
  
  METHODS
  Under the condition with or without serum, MC3T3-E1 cells were stimulated with 10 μg/mL P.e-LPS for 24 hours, then the expression of CD14 was measured using flow cytometry; the membrane CD14 mRNA was detected at different time point (0, 1, 3, 6, 12, 24 h) by RT-PCR. Statistical analysis was performed using two-sample t test, one-way ANOVA and Dunnett t test with SPSS13.0 software package.
  
  
  RESULTS
  Flow cytometry showed that CD14 protein increased after the stimulation of P.e-LPS for 24 h, while the non-serum group demonstrated more increase; the membrane CD14 mRNA level was up-regulated by 10 μg/mL P.e-LPS at 1 hour, and reached the maximum at 3 h and declined after 6 h.
  
  
  CONCLUSIONS
  P.e-LPS can induce the expression of CD14 in osteoblast MC3T3-E1, which indicates that P.e-LPS may play an important role in osteoblast through CD14.
authors.affiliation: Department of Endodontics, College of Stomatology, China Medical University, Shenyang 110002, Liaoning Province, China. 1985jiage@163.com
authors.forename: Ge
authors.initials: G
authors.lastname: Jia
authors.forename: Li-hong
authors.initials: LH
authors.lastname: Qiu
authors.forename: Ren
authors.initials: R
authors.lastname: Li
authors.forename: Di
authors.initials: D
authors.lastname: Yang
authors.forename: Yan
authors.initials: Y
authors.lastname: Guo
mesh_terms: D006801:Humans; D008070:Lipopolysaccharides; D010006:Osteoblasts; D045202:Porphyromonas endodontalis; D012333:RNA, Messenger
chemical_list: D008070:Lipopolysaccharides; D012333:RNA, Messenger