title: Top down and bottom up engineering of bone.
abstract: The goal of this retrospective article is to place the body of my lab's multiscale mechanobiology work in context of top-down and bottom-up engineering of bone. We have used biosystems engineering, computational modeling and novel experimental approaches to understand bone physiology, in health and disease, and across time (in utero, postnatal growth, maturity, aging and death, as well as evolution) and length scales (a single bone like a femur, m; a sample of bone tissue, mm-cm; a cell and its local environment, μm; down to the length scale of the cell's own skeleton, the cytoskeleton, nm). First we introduce the concept of flow in bone and the three calibers of porosity through which fluid flows. Then we describe, in the context of organ-tissue, tissue-cell and cell-molecule length scales, both multiscale computational models and experimental methods to predict flow in bone and to understand the flow of fluid as a means to deliver chemical and mechanical cues in bone. Addressing a number of studies in the context of multiple length and time scales, the importance of appropriate boundary conditions, site specific material parameters, permeability measures and even micro-nanoanatomically correct geometries are discussed in context of model predictions and their value for understanding multiscale mechanobiology of bone. Insights from these multiscale computational modeling and experimental methods are providing us with a means to predict, engineer and manufacture bone tissue in the laboratory and in the human body.
authors.affiliation: Department of Mechanical & Aerospace Engineering, Department of Biomedical Engineering, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106-7222, USA. knothetate@case.edu
authors.forename: Melissa L
authors.initials: ML
authors.lastname: Knothe Tate
mesh_terms: D000818:Animals; D016880:Anisotropy; D001842:Bone and Bones / Q000033:anatomy & histology / Q000502:physiology*; D003198:Computer Simulation; D003599:Cytoskeleton / Q000502:physiology; D006801:Humans; D008954:Models, Biological; D015233:Models, Statistical; D019904:Polymethyl Methacrylate / Q000737:chemistry; D016062:Porosity; D051381:Rats; D013314:Stress, Mechanical; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D019904:Polymethyl Methacrylate