title: [Influence and distribution of CD133 and CD34 antigen expression in proximal tubule epithelial cells during ischemia/reperfusion injury in rats].
abstract: OBJECTIVE
  To study the influence and distribution in proximal tubule epithelial cells with the expressions of CD133 and CD34 in a rat model so as to provide a study basis for renal adult stem cell.
  
  
  METHODS
  The kidney ischemia/reperfusion model was established by blocking the bilateral renal arteries for about 50 min and recovering the renal perfusion of blood. Then the rat kidneys were extracted at Days 3, 5 and 7 post-modeling. After a series of special treatment, immunohistochemical staining was used to show the distribution and expression intensity of KIM-1, Brdu antigen, CD34 and CD133 antigens in cells with the elapsing of time.
  
  
  RESULTS
  As compared with control group, the KIM-1 and CD133 antigens were present in cortex renis while the CD34 and Brdu antigens were distributed in parts of medulla renis and juncture of cortex-medulla renis. The expression density value of KIM-1 and CD133 antigens rose for the first 3 days then declined afterward (40.3% ± 3.2%, 57.5% ± 3.8%, 24.3% ± 1.4%). Otherwise the expression density value of CD 34 antigen declined (56.0% ± 4.8%, 44.2% ± 2.2%, 28.8% ± 1.0%) and Brdu antigen showed an upward trend at post-operation (10.0% ± 1.1%, 36.0% ± 4.2%, 48.8% ± 5.0%).
  
  
  CONCLUSIONS
  After ischemia/reperfusion injury in kidney, the expression rates of CD133 and KIM-1 antigen rise obviously in cortex renis. And the D34 and Brdu antigens show a similar trend in medulla renis. The result indicates that the phenomenon of proliferation and differentiation may appears in kidney proximal tubule and migrate from the region of medulla renis to cortex renis. The participating cells not only possess the strong proliferation and repairing ability of stem/progenitor cells, but also can expresses the CD34 and CD133 antigens. Thus it is may provide a study basis for the tissue reconstruction of nephron and research in the field of kidney adult stem cell.
authors.affiliation: Department of Urology, Affiliated Hospital of Guiyang Medical College, Guiyang 550004, China. cfm_cn@yahoo.com.cn
authors.forename: Fang-min
authors.initials: FM
authors.lastname: Chen
authors.forename: Xi-nan
authors.initials: XN
authors.lastname: Jiang
authors.forename: Jia-qi
authors.initials: JQ
authors.lastname: Shi
authors.forename: Bo
authors.initials: B
authors.lastname: Yan
authors.forename: De-shuai
authors.initials: DS
authors.lastname: Ren
authors.forename: Jiang
authors.initials: J
authors.lastname: Gu
authors.forename: Deng-bao
authors.initials: DB
authors.lastname: Li
authors.forename: Ya
authors.initials: Y
authors.lastname: Zhang
authors.forename: Jun
authors.initials: J
authors.lastname: Shen
authors.forename: Jun
authors.initials: J
authors.lastname: Liang
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000071916:AC133 Antigen; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism*; D018952:Antigens, CD34 / Q000378:metabolism*; D004847:Epithelial Cells / Q000378:metabolism; D005260:Female; D006023:Glycoproteins / Q000378:metabolism*; D007687:Kidney Tubules, Proximal / Q000378:metabolism*; D008297:Male; D010455:Peptides / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000378:metabolism*
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D018952:Antigens, CD34; D006023:Glycoproteins; D010455:Peptides; C000606025:Prom1 protein, rat