title: Sequence analysis of the genome of an oil-bearing tree, Jatropha curcas L.
abstract: The whole genome of Jatropha curcas was sequenced, using a combination of the conventional Sanger method and new-generation multiplex sequencing methods. Total length of the non-redundant sequences thus obtained was 285 858 490 bp consisting of 120 586 contigs and 29 831 singlets. They accounted for ~95% of the gene-containing regions with the average G + C content was 34.3%. A total of 40 929 complete and partial structures of protein encoding genes have been deduced. Comparison with genes of other plant species indicated that 1529 (4%) of the putative protein-encoding genes are specific to the Euphorbiaceae family. A high degree of microsynteny was observed with the genome of castor bean and, to a lesser extent, with those of soybean and Arabidopsis thaliana. In parallel with genome sequencing, cDNAs derived from leaf and callus tissues were subjected to pyrosequencing, and a total of 21 225 unigene data have been generated. Polymorphism analysis using microsatellite markers developed from the genomic sequence data obtained was performed with 12 J. curcas lines collected from various parts of the world to estimate their genetic diversity. The genomic sequence and accompanying information presented here are expected to serve as valuable resources for the acceleration of fundamental and applied research with J. curcas, especially in the fields of environment-related research such as biofuel production. Further information on the genomic sequences and DNA markers is available at http://www.kazusa.or.jp/jatropha/.
authors.affiliation: Kazusa DNA Research Institute, 2-6-7 Kazusa-kamatari, Kisarazu, Chiba, Japan.
authors.forename: Shusei
authors.initials: S
authors.lastname: Sato
authors.forename: Hideki
authors.initials: H
authors.lastname: Hirakawa
authors.forename: Sachiko
authors.initials: S
authors.lastname: Isobe
authors.forename: Eigo
authors.initials: E
authors.lastname: Fukai
authors.forename: Akiko
authors.initials: A
authors.lastname: Watanabe
authors.forename: Midori
authors.initials: M
authors.lastname: Kato
authors.forename: Kumiko
authors.initials: K
authors.lastname: Kawashima
authors.forename: Chiharu
authors.initials: C
authors.lastname: Minami
authors.forename: Akiko
authors.initials: A
authors.lastname: Muraki
authors.forename: Naomi
authors.initials: N
authors.lastname: Nakazaki
authors.forename: Chika
authors.initials: C
authors.lastname: Takahashi
authors.forename: Shinobu
authors.initials: S
authors.lastname: Nakayama
authors.forename: Yoshie
authors.initials: Y
authors.lastname: Kishida
authors.forename: Mitsuyo
authors.initials: M
authors.lastname: Kohara
authors.forename: Manabu
authors.initials: M
authors.lastname: Yamada
authors.forename: Hisano
authors.initials: H
authors.lastname: Tsuruoka
authors.forename: Shigemi
authors.initials: S
authors.lastname: Sasamoto
authors.forename: Satoshi
authors.initials: S
authors.lastname: Tabata
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Aizu
authors.forename: Atsushi
authors.initials: A
authors.lastname: Toyoda
authors.forename: Tadasu
authors.initials: T
authors.lastname: Shin-i
authors.forename: Yohei
authors.initials: Y
authors.lastname: Minakuchi
authors.forename: Yuji
authors.initials: Y
authors.lastname: Kohara
authors.forename: Asao
authors.initials: A
authors.lastname: Fujiyama
authors.forename: Suguru
authors.initials: S
authors.lastname: Tsuchimoto
authors.forename: Shin'ichiro
authors.initials: S
authors.lastname: Kajiyama
authors.forename: Eri
authors.initials: E
authors.lastname: Makigano
authors.forename: Nobuko
authors.initials: N
authors.lastname: Ohmido
authors.forename: Nakako
authors.initials: N
authors.lastname: Shibagaki
authors.forename: Joyce A
authors.initials: JA
authors.lastname: Cartagena
authors.forename: Naoki
authors.initials: N
authors.lastname: Wada
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Kohinata
authors.forename: Alipour
authors.initials: A
authors.lastname: Atefeh
authors.forename: Shota
authors.initials: S
authors.lastname: Yuasa
authors.forename: Sachihiro
authors.initials: S
authors.lastname: Matsunaga
authors.forename: Kiichi
authors.initials: K
authors.lastname: Fukui
mesh_terms: D018745:Genome, Plant; D031286:Jatropha / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D010940:Plant Proteins