title: Removal of bacteria from boar ejaculates by Single Layer Centrifugation can reduce the use of antibiotics in semen extenders.
abstract: There is considerable interest world-wide in reducing the use of antibiotics to stem the development of antibiotic-resistant strains of bacteria. An alternative to the routine addition of antibiotics to semen extenders in livestock breeding would be to separate the spermatozoa from bacterial contaminants in the semen immediately after collection. The present study was designed to determine whether such separation was possible by Single Layer Centrifugation (SLC) using the colloid Androcoll™-P. The results showed that complete removal (6 out of 10 samples), or considerable reduction of bacterial contaminants (4 out of 10 samples) was possible with this method. The type of bacteria and/or the length of time between collection and SLC-processing affected the removal of bacteria, with motile flagellated bacteria being more likely to be present after SLC than non-flagellated bacteria. Although further studies are necessary, these preliminary results suggest that the use of SLC when processing boar semen for AI doses might enable antibiotic usage in semen extenders to be reduced.
authors.affiliation: Division of Reproduction, Department of Clinical Sciences, Swedish University of Agricultural Sciences-SLU, Uppsala, Sweden. jane.morrell@kv.slu.se
authors.forename: J M
authors.initials: JM
authors.lastname: Morrell
authors.forename: M
authors.initials: M
authors.lastname: Wallgren
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001419:Bacteria / Q000302:isolation & purification*; D058491:Bacterial Load; D002469:Cell Separation / Q000379:methods*; D002498:Centrifugation / Q000379:methods; D003451:Cryoprotective Agents / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004542:Ejaculation / Q000502:physiology; D008297:Male; D012661:Semen / Q000166:cytology* / Q000382:microbiology*; D055101:Semen Analysis; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary; D013552:Swine / Q000502:physiology
chemical_list: D000900:Anti-Bacterial Agents; D003451:Cryoprotective Agents