title: On-chip electro membrane extraction with online ultraviolet and mass spectrometric detection.
abstract: Electro membrane extraction was demonstrated in a microfluidic device. The device was composed of a 25 μm thick porous polypropylene membrane bonded between two poly(methyl methacrylate) (PMMA) substrates, each having 50 μm deep channel structures facing the membrane. The supported liquid membrane (SLM) consisted of 2-nitrophenyl octyl ether (NPOE) immobilized in the pores of the membrane. The driving force for the extraction was a 15 V direct current (DC) electrical potential applied across the SLM. Samples containing the basic drugs pethidine, nortriptyline, methadone, haloperidol, loperamide, and amitriptyline were used to characterize the system. Extraction recoveries were typically in the range of 65-86% for the different analytes when the device was operated with a sample flow of 2.0 μL/min and an acceptor flow of 1.0 μL/min. With the sample flow at 9.0 μL/min and the acceptor flow at 0.0 μL/min, enrichment factors exceeding 75 were obtained during 12 min of operation from a total sample volume of only 108 μL. The on-chip electro membrane system was coupled online to electrospray ionization mass spectrometry and used to monitor online and real-time metabolism of amitriptyline by rat liver microsomes.
authors.affiliation: Department of Pharmaceutics and Analytical Chemistry, Faculty of Pharmaceutical Sciences, University of Copenhagen, DK-2100 Copenhagen, Denmark. njp@farma.ku.dk
authors.forename: Nickolaj Jacob
authors.initials: NJ
authors.lastname: Petersen
authors.forename: Sunniva Taule
authors.initials: ST
authors.lastname: Foss
authors.forename: Henrik
authors.initials: H
authors.lastname: Jensen
authors.forename: Steen Honoré
authors.initials: SH
authors.lastname: Hansen
authors.forename: Christian
authors.initials: C
authors.lastname: Skonberg
authors.forename: Detlef
authors.initials: D
authors.lastname: Snakenborg
authors.forename: Jörg P
authors.initials: JP
authors.lastname: Kutter
authors.forename: Stig
authors.initials: S
authors.lastname: Pedersen-Bjergaard
mesh_terms: D000639:Amitriptyline / Q000378:metabolism; D000818:Animals; D005591:Chemical Fractionation / Q000295:instrumentation*; D008297:Male; D008567:Membranes, Artificial; D008691:Methadone / Q000302:isolation & purification / Q000652:urine; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D008862:Microsomes, Liver / Q000378:metabolism; D009862:Online Systems; D011126:Polypropylenes / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
chemical_list: D008567:Membranes, Artificial; D011126:Polypropylenes; D000639:Amitriptyline; D008691:Methadone