title: Microarray analysis of the chelerythrine-induced transcriptome of Mycobacterium tuberculosis.
abstract: Chelerythrine (a natural quaternary benzophenanthridine alkaloid) is an extract from the roots of Chelidonium majus with potential antimycobacterial activity. To reveal the possible mechanism of action of chelerythrine against Mycobacterium tuberculosis (M. tuberculosis), commercial oligonucleotide microarrays were used to analyze the genome-wide transcriptional changes triggered by treatment with subinhibitory concentrations of chelerythrine. Quantitative real-time RT-PCR was performed for selected genes to verify the microarray results. We interpreted our microarray data using Agilent software. Analysis of the microarray data revealed that a total of 759 genes were differentially regulated by chelerythrine. Of these, 372 genes were upregulated, and 387 genes were downregulated. Some of the important genes that were significantly regulated are related to different pathways (such as urease), methoxy-mycolic acid synthase, surface-exposed lipids, the heat shock response, and protein synthesis. This genome-wide transcriptomics approach produced the first insights into the response of M. tuberculosis to a chelerythrine challenge.
authors.affiliation: Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, China.
authors.forename: Junchao
authors.initials: J
authors.lastname: Liang
authors.forename: Fanli
authors.initials: F
authors.lastname: Zeng
authors.forename: Aizhen
authors.initials: A
authors.lastname: Guo
authors.forename: Liqiang
authors.initials: L
authors.lastname: Liu
authors.forename: Na
authors.initials: N
authors.lastname: Guo
authors.forename: Lei
authors.initials: L
authors.lastname: Li
authors.forename: Jing
authors.initials: J
authors.lastname: Jin
authors.forename: Xiuping
authors.initials: X
authors.lastname: Wu
authors.forename: Mingyuan
authors.initials: M
authors.lastname: Liu
authors.forename: Dan
authors.initials: D
authors.lastname: Zhao
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.forename: Qi
authors.initials: Q
authors.lastname: Jin
authors.forename: Lu
authors.initials: L
authors.lastname: Yu
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D053119:Benzophenanthridines / Q000494:pharmacology*; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D046228:Microarray Analysis; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000235:genetics* / Q000378:metabolism; D028101:Papaveraceae / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*
chemical_list: D001426:Bacterial Proteins; D053119:Benzophenanthridines; D010936:Plant Extracts; C016299:chelerythrine