title: Retinal thinning in tree shrews with induced high myopia: optical coherence tomography and histological assessment.
abstract: This study determined retinal thinning in a mammalian model of high myopia using optical coherence tomography (OCT) and histological sections from the same retinal tissue. High myopia was induced in three tree shrews (Tupaia belangeri) by deprivation of form vision via lid suture of one eye, with the other eye a control. Ocular biometry data was obtained by Ascan ultrasonography, keratometry and retinoscopy. The Zeiss StratusOCT was used to obtain Bscans in vivo across the retina. Subsequently, eyes were enucleated and retinas fixed, dehydrated, embedded and sectioned. Treated eyes developed a high degree of axial myopia (-15.9 ± 2.3D; n = 3). The OCT analysis showed that in myopic eyes the nasal retina thinned more than the temporal retina relative to the disc (p=0.005). Histology showed that the retinas in the myopic eyes comprise all layers but were thinner than the retinas in normal and control eyes. Detailed thickness measurements in corresponding locations of myopic and control eyes in superior nasal retina using longitudinal reflectivity profiles from OCT and semithin vertical histological sections showed the percentage of retinal thinning in the myopic eyes was similar between methods (OCT 15.34 ± 5.69%; histology 17.61 ± 3.02%; p = 0.10). Analysis of retinal layers revealed that the inner plexiform, inner nuclear and outer plexiform layers thin the most. Cell density measurements showed all neuronal cell types are involved in retinal thinning. The results indicate that in vivo OCT measurements can accurately detect retinal thinning in high myopia.
authors.affiliation: Department of Optometry and Vision Sciences, The University of Melbourne, Parkville, Victoria 3010, Australia. carla.abbott@sydney.edu.au
authors.forename: Carla J
authors.initials: CJ
authors.lastname: Abbott
authors.forename: Ulrike
authors.initials: U
authors.lastname: Grünert
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Pianta
authors.forename: Neville A
authors.initials: NA
authors.lastname: McBrien
mesh_terms: D000818:Animals; D001699:Biometry; D004195:Disease Models, Animal; D009216:Myopia / Q000503:physiopathology*; D012160:Retina / Q000473:pathology*; D041623:Tomography, Optical Coherence; D014417:Tupaia