title: [Improve PCR-select differential screening kit with digoxin labelled probe].
abstract: OBJECTIVE
  To explore a method to replace the isotope probe in the PCR-Select differential screening Kit with DIG labeled probe.
  
  
  METHODS
  Differential expressed sequences isolated from Suppression Subtractive Hybridization (SSH) was translocated onto nitric fibrin film. Probes were prepared with DIG-11-dUTP mixed in by PCR. Hybridization and coloration followed routine operation procedures of DIG labeled probe. Positive hybridization results were verified with reverse Northern blot.
  
  
  RESULTS
  The positive results from the PCR-Select differential screening Kit improved with DIG labeled probe achieved 90% correspondence verified by reverse Northern blot.
  
  
  CONCLUSIONS
  The PCR-Select differential screening Kit improved with DIG labeled probe maintained high specificity while avoiding isotope pollution. It can replace isotope probe completely.
authors.affiliation: Department of Tropical Medicine, the First Military Medical University, Guangzhou 510515, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Xiao
authors.forename: Fei
authors.initials: F
authors.lastname: Zou
authors.forename: Shao-xi
authors.initials: SX
authors.lastname: Cai
mesh_terms: D015342:DNA Probes; D004077:Digoxin; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction / Q000295:instrumentation* / Q000379:methods*; D011933:Reagent Kits, Diagnostic
chemical_list: D015342:DNA Probes; D011933:Reagent Kits, Diagnostic; D004077:Digoxin