title: Expression of AMIDASE1 (AMI1) is suppressed during the first two days after germination.
abstract: The regulation of cellular auxin levels is a critical factor in determining plant growth and architecture, as indole-3-acetic acid (IAA) gradients along the plant axis and local IAA maxima are known to initiate numerous plant growth responses. The regulation of auxin homeostasis is mediated in part by transport, conjugation and deconjugation, as well as by de novo biosynthesis. However, the pathways of IAA biosynthesis are yet not entirely characterized at the molecular and biochemical level. It is suggested that several biosynthetic routes for the formation of IAA have evolved. One such pathway proceeds via the intermediate indole-3-acetamide (IAM), which is converted into IAA by the activity of specific IAM hydrolases, such as Arabidopsis AMIDASE1 (AMI1). In this article we present evidence to support the argument that AMI1-dependent IAA synthesis is likely not to be used during the first two days of seedling development.
authors.affiliation: Centro de Biotecnología y Genómica de Plantas (U.P.M. - I.N.I.A.), Campus de Montegancedo, Pozuelo de Alarcón (Madrid), Spain.
authors.forename: Maik
authors.initials: M
authors.lastname: Hoffmann
authors.forename: Thomas
authors.initials: T
authors.lastname: Lehmann
authors.forename: Daniel
authors.initials: D
authors.lastname: Neu
authors.forename: Mathias
authors.initials: M
authors.lastname: Hentrich
authors.forename: Stephan
authors.initials: S
authors.lastname: Pollmann
mesh_terms: D000581:Amidohydrolases / Q000037:antagonists & inhibitors*; D017360:Arabidopsis / Q000201:enzymology* / Q000254:growth & development; D018525:Germination; D007210:Indoleacetic Acids / Q000378:metabolism*
chemical_list: D007210:Indoleacetic Acids; C015950:indoleacetamide; D000581:Amidohydrolases; C019665:amidase