title: Roles of the ribosomal protein S19 dimer and chemically induced apoptotic cells as a tumor vaccine in syngeneic mouse transplantation models.
abstract: We examined the roles of a chemotherapeutic reagent in both inducing apoptosis and conferring acquired tumor immunity using mouse syngeneic tumor transplantation models. A chemotherapeutic reagent, cis-diamminedichloroplatinum (II), effectively induced apoptosis in Colon-26 cells originating from a BALB/c mouse. Three intradermal inoculations of cis-diamminedichloroplatinum (II)-treated Colon-26 cells conferred tumor immunity against viable Colon-26 cells in BALB/c mice but not in athymic BALB/c mice. The acquired immunity was Colon-26 cell specific and was adoptively transferable with splenic cells. We next examined the involvement of the cross-linked ribosomal protein S19 dimer (RP S19), which is released during apoptosis, in the acquisition of tumor immunity. A Colon-26 transformant that produces a Gln137Asn-mutant RP S19 prevents the formation of a functional RP S19 dimer. Acquired tumor immunity was significantly reduced when the transformant was used in combination with anti-RP S19 antibodies as the vaccination source. These data suggest the importance of the RP S19 dimer in chemotherapeutic agent-induced acquired immunity.
authors.affiliation: Faculty of Life Science, Department of Molecular Pathology, Kumamoto University, Japan.
authors.forename: Keisuke
authors.initials: K
authors.lastname: Taniguchi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nishiura
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Ota
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Yamamoto
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D017209:Apoptosis; D015153:Blotting, Western; D019496:Cancer Vaccines / Q000276:immunology*; D045744:Cell Line, Tumor; D002945:Cisplatin / Q000494:pharmacology; D016219:Immunotherapy, Adoptive; D020287:In Situ Nick-End Labeling; D007271:Injections, Intradermal; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009154:Mutation; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental; D055503:Protein Multimerization; D012269:Ribosomal Proteins / Q000276:immunology*; D015398:Signal Transduction; D014185:Transplantation, Isogeneic
chemical_list: D019496:Cancer Vaccines; D012269:Ribosomal Proteins; C040343:ribosomal protein S19; D002945:Cisplatin