title: Label-free, arrayed sensing of immune response to influenza antigens.
abstract: Periodic outbreaks of pandemic influenza have been a devastating cause of human mortality over the past century. More recently, an avian influenza strain, designated H5N1, has been identified as having the potential to cause a zoogenic pandemic in humans, and a current outbreak of a new H1N1 influenza variant hypothesized to be of swine origin is of considerable concern. In order to facilitate surveillance and the rapid assessment and comparison of vaccination efforts, a high-throughput assay is highly desirable to supplement standard methods, which require high biosafety-level facilities. In this paper, we describe the design, production, and preliminary evaluation of an antigen array incorporating a panel of hemagglutinins as a platform for the detection and rapid quantification of influenza-specific antibodies in human serum by Arrayed Imaging Reflectometry (AIR), a label-free optical biosensor.
authors.affiliation: Department of Biochemistry and Biophysics, University of Rochester, Rochester, NY 14642, USA.
authors.forename: Charles R
authors.initials: CR
authors.lastname: Mace
authors.forename: David J
authors.initials: DJ
authors.lastname: Topham
authors.forename: Tim R
authors.initials: TR
authors.lastname: Mosmann
authors.forename: Sally A
authors.initials: SA
authors.lastname: Quataert
authors.forename: John J
authors.initials: JJ
authors.lastname: Treanor
authors.forename: Benjamin L
authors.initials: BL
authors.lastname: Miller
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000276:immunology*; D015374:Biosensing Techniques / Q000295:instrumentation*; D003429:Cross Reactions; D005260:Female; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000276:immunology; D006801:Humans; D053124:Influenza A Virus, H5N1 Subtype / Q000276:immunology*; D007252:Influenza Vaccines / Q000276:immunology; D051379:Mice; D046228:Microarray Analysis / Q000379:methods*
chemical_list: D000956:Antigens, Viral; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D007252:Influenza Vaccines