title: Characterization of the small RNA transcriptomes of androgen dependent and independent prostate cancer cell line by deep sequencing.
abstract: Given the important roles of miRNA in post-transcriptional regulation and its implications for cancer, characterization of miRNA facilitates us to uncover molecular mechanisms underlying the progression of androgen-independent prostate cancer (PCa). The emergence of next-generation sequencing technologies has dramatically changed the speed of all aspects of sequencing in a rapid and cost-effective fashion, which can permit an unbiased, quantitive and in-depth investigation of small RNA transcriptome. In this study, we used high-throughput Illumina sequencing to comprehensively represent the full complement of individual small RNA and to characterize miRNA expression profiles in both the androgen dependent and independent Pca cell line. At least 83 miRNAs are significantly differentially expressed, of which 41 are up-regulated and 42 are down-regulated, indicating these miRNAs may be involved in the transition of LNCaP to an androgen-independent phenotype. In addition, we have identified 43 novel miRNAs from the androgen dependent and independent PCa library and 3 of them are specific to the androgen-independent PCa. Function annotation of target genes indicated that most of these differentially expressed miRNAs tend to target genes involved in signal transduction and cell communication, epically the MAPK signaling pathway. The small RNA transcriptomes obtained in this study provide considerable insights into a better understanding of the expression and function of small RNAs in the development of androgen-independent prostate cancer.
authors.affiliation: Center for Clinical Laboratory Diagnosis, the First Affiliated Hospital of Wenzhou Medical College, Wenzhou, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Xu
authors.forename: Jinyu
authors.initials: J
authors.lastname: Wu
authors.forename: LingLin
authors.initials: L
authors.lastname: Zhou
authors.forename: Binghua
authors.initials: B
authors.lastname: Chen
authors.forename: Zhongsheng
authors.initials: Z
authors.lastname: Sun
authors.forename: Fangqing
authors.initials: F
authors.lastname: Zhao
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Tao
mesh_terms: D000728:Androgens / Q000378:metabolism; D045744:Cell Line, Tumor; D002874:Chromosome Mapping; D020869:Gene Expression Profiling; D015723:Gene Library; D015894:Genome, Human / Q000235:genetics; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D018398:Homeodomain Proteins / Q000235:genetics; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D009419:Nerve Tissue Proteins / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D011471:Prostatic Neoplasms / Q000235:genetics / Q000473:pathology; D051057:Proto-Oncogene Proteins c-akt / Q000235:genetics; D016601:RNA-Binding Proteins / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000235:genetics; D000071799:Zinc Finger E-box-Binding Homeobox 1
chemical_list: D000728:Androgens; C108381:GEMIN2 protein, human; D018398:Homeodomain Proteins; D035683:MicroRNAs; D009419:Nerve Tissue Proteins; D016601:RNA-Binding Proteins; D014157:Transcription Factors; C486307:ZEB1 protein, human; D000071799:Zinc Finger E-box-Binding Homeobox 1; C491754:AKT3 protein, human; D051057:Proto-Oncogene Proteins c-akt