title: [Primary monolayer culture of rat adrenal cortical cells].
abstract: OBJECTIVE
  To culture adrenal cortical cells of rat in primary monolayer form.
  
  
  METHODS
  Collagenase and hyaluronidase incubation were used to isolate adrenocortical cells of male Wistar rats. The isolated adrenocortical cells were incubated in the DMEM solution supplied with 15% NBS, at the temperature of 37 degrees C, and the air contained 5% CO2. Identification was done by 3bet-HSD dyeing.
  
  
  RESULTS
  The cells which had the relatively big cell body and a few projections were dyed. There was a phenomenon of coexistence of epithelial-like cells and fibroblastic cells.
  
  
  CONCLUSIONS
  Rats monolayer adrenocortical cell culture was successful.
authors.affiliation: Institute of Aviation Medicine, Air Force, Beijing 100036, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Guo
authors.forename: Hong-tao
authors.initials: HT
authors.lastname: Liu
mesh_terms: D000302:Adrenal Cortex / Q000166:cytology*; D000818:Animals; D002478:Cells, Cultured; D008297:Male; D061251:Primary Cell Culture; D051381:Rats; D017208:Rats, Wistar