title: The ins and outs of hematopoietic stem cells: studies to improve transplantation outcomes.
abstract: Deciphering the mechanisms of hematopoietic stem/progenitor cell (HSPC) mobilization and homing is important for the development of strategies to enhance the efficacy of HSPC transplantation and achieve the full potential of HSPC-based cellular therapy. Investigation of these mechanisms has revealed interdependence among the various molecules, pathways and cellular components involved, and underscored the complex nature of these two processes. This review summarizes recent progress in identifying the specific factors implicated in HSPC mobilization and homing, with emphasis on our own work. Particularly, we will discuss our studies on stromal cell-derived factor-1 and its interaction with its receptor CXCR4, proteases (matrix metalloproteinases and carboxypeptidase M), complement proteins (C1q, C3a, C5a, membrane attack complex), sphingosine-1-phosphate, and pharmacologic agents such as the histone deacetylase inhibitor valproic acid and hyaluronic acid.
authors.affiliation: Research & Development, Canadian Blood Services, CBS Edmonton Centre, 8249-114 St. NW, Edmonton, T6G 2R8, Alberta, Canada.
authors.forename: Leah A
authors.initials: LA
authors.lastname: Marquez-Curtis
authors.forename: A Robert
authors.initials: AR
authors.lastname: Turner
authors.forename: Santhi
authors.initials: S
authors.lastname: Sridharan
authors.forename: Mariusz Z
authors.initials: MZ
authors.lastname: Ratajczak
authors.forename: Anna
authors.initials: A
authors.lastname: Janowska-Wieczorek
mesh_terms: D000818:Animals; D015815:Cell Adhesion Molecules / Q000378:metabolism; D064987:Cell- and Tissue-Based Therapy / Q000379:methods*; D003165:Complement System Proteins / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D019650:Hematopoietic Stem Cell Mobilization; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000502:physiology*; D006801:Humans; D007113:Immunity, Innate; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D020782:Matrix Metalloproteinases / Q000378:metabolism; D055153:Stem Cell Niche; D016896:Treatment Outcome
chemical_list: D015815:Cell Adhesion Molecules; D016207:Cytokines; D036341:Intercellular Signaling Peptides and Proteins; D003165:Complement System Proteins; D020782:Matrix Metalloproteinases