title: Influence of lipids on the interfacial disposition of respiratory syncytical virus matrix protein.
abstract: The propensity of a matrix protein from an enveloped virus of the Mononegavirales family to associate with lipids representative of the viral envelope has been determined using label-free methods, including tensiometry and Brewster angle microscopy on lipid films at the air-water interface and atomic force microscopy on monolayers transferred to OTS-treated silicon wafers. This has enabled factors that influence the disposition of the protein with respect to the lipid interface to be characterized. In the absence of sphingomyelin, respiratory syncytial virus matrix protein penetrates monolayers composed of mixtures of phosphocholines with phosphoethanolamines or cholesterol at the air-water interface. In ternary mixtures composed of sphingomyelin, 1,2-dioleoyl-sn-glycero-3-phosphocholine, and cholesterol, the protein exhibits two separate behaviors: (1) peripheral association with the surface of sphingomyelin-rich domains and (2) penetration of sphingomyelin-poor domains. Prolonged incubation of the protein with mixtures of phosphocholines and phosphoethanolamines leads to the formation of helical protein assemblies of uniform diameter that demonstrate an inherent propensity of the protein to assemble into a filamentous form.
authors.affiliation: Department of Chemistry and Biophysical Sciences Institute, Durham University, South Road, Durham DH1 3LE, United Kingdom.
authors.forename: Helen K
authors.initials: HK
authors.lastname: McPhee
authors.forename: Jennifer L
authors.initials: JL
authors.lastname: Carlisle
authors.forename: Andrew
authors.initials: A
authors.lastname: Beeby
authors.forename: Victoria A
authors.initials: VA
authors.lastname: Money
authors.forename: Scott M D
authors.initials: SM
authors.lastname: Watson
authors.forename: R Paul
authors.initials: RP
authors.lastname: Yeo
authors.forename: John M
authors.initials: JM
authors.lastname: Sanderson
mesh_terms: D000327:Adsorption; D018360:Crystallography, X-Ray; D008055:Lipids / Q000737:chemistry*; D008853:Microscopy; D012136:Respiratory Syncytial Viruses; D013499:Surface Properties; D014764:Viral Proteins / Q000737:chemistry* / Q000378:metabolism
chemical_list: D008055:Lipids; D014764:Viral Proteins