title: [Three subtypes expressions of leptin receptor in the rat anterior pituitary and influence of leptin on intracellular free Ca2+ of the rat growth hormone cell].
abstract: OBJECTIVE
  To observe the expression of Leptin receptors (OB-R) in male rat anterior pituitary, and study the influence of Leptin on the level of intracellular free Ca2+ ([Ca2+]i) in the cultured growth hormone (GH) cell of male rat pituitary.
  
  
  METHODS
  RT-PCR method was used to observe the expression of Leptin receptors (OB-R) in male rat anterior pituitary. We used grade centrifuging method to get growth hormone (GH) cell, and [Ca2+]i in GH cell was examined by laser scanning confocal system.
  
  
  RESULTS
  OB-R mRNA were expressed in male rat anterior pituitary, including OB-R (common form), OB-Ra (short form) and OB-Rb (long form). There were about 70% or 80% GH cell by grade centrifuging. Leptin at 10(-8)mol/L could decrease the level intracellular free Ca2+ ([Ca2+]i) in cultured GH cell.
  
  
  CONCLUSIONS
  There are three subtypes of Leptin receptors expressions in male rat anterior pituitary, and Leptin could reduce intracellular free Ca2+ level of GH cell markedly.
authors.affiliation: Department of Physiology, Faculty of Preclinical Medicine, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Ya-Li
authors.initials: YL
authors.lastname: Liu
authors.forename: Yan-Qing
authors.initials: YQ
authors.lastname: Zhong
authors.forename: Yun-Long
authors.initials: YL
authors.lastname: Zhu
authors.forename: Su-Min
authors.initials: SM
authors.lastname: Chi
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D013006:Growth Hormone / Q000378:metabolism; D008297:Male; D010902:Pituitary Gland / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011956:Receptors, Cell Surface / Q000378:metabolism; D054411:Receptors, Leptin / Q000378:metabolism*
chemical_list: D011956:Receptors, Cell Surface; D054411:Receptors, Leptin; D013006:Growth Hormone; D002118:Calcium