title: [Subtype and transmission of HIV strain in both HIV infected spouses in Zhejiang province].
abstract: OBJECTIVE
  To analyse the subtype and transmission of HIV strain in both HIV infected spouses.
  
  
  METHODS
  Reported both HIV infected spouses were selected as objects. Analysis on genetic sequence and high risk behaviors was carried out. Subgenomic gag was amplified by nest-PCR analysis of whole blood samples from objects. Genetic subtype characterization of HIV was identified and pairwise genetic distances were calculated. Sequence relationships were also examined by phylogenetic tree analysis.
  
  
  RESULTS
  Genetic sequences of 46 pairs (92 cases) were obtained. The genetic subtype comprised 39 CRF01_AE (42.4%), 10 CRF07_BC (10.9%), 18 CRF08_BC (19.6%), 18 B (19.6%), 5 C (5.4%) and 2 CRF02_AG (2.2%). 44 pairs had the same subtype between the two partners, accounted for 95.7% of the total. 33 of the 41 pairs with phylogenetic tree analysis were found having epidemiological linkage in pair wise. Sexual behaviors of out-marriage were the main risk factors of CRF01_AE and CRF08_BC and CRF02_AG strains infection. Blood transmission was associated with B and CRF07_BC.
  
  
  CONCLUSIONS
  The HIV strains subtype detected in HIV infected spouses characterized with diversity. CRF01_AE was the main strain subtype. The main route of transmission to the spouses was through unprotected sexual contacts. Surveillance programs on HIV infected partner together with intervention between the spouses should be improved.
authors.affiliation: Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China. xhpan310009@yahoo.com.cn
authors.forename: Xiao-hong
authors.initials: XH
authors.lastname: Pan
authors.forename: Jia-feng
authors.initials: JF
authors.lastname: Zhang
authors.forename: Ya-ping
authors.initials: YP
authors.lastname: Yao
authors.forename: Yun
authors.initials: Y
authors.lastname: Xu
authors.forename: Jie-zhe
authors.initials: JZ
authors.lastname: Yang
authors.forename: Zhi-hong
authors.initials: ZH
authors.lastname: Guo
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000453:epidemiology / Q000635:transmission* / Q000821:virology*; D000328:Adult; D002681:China / Q000453:epidemiology; D005260:Female; D005838:Genotype; D015497:HIV-1 / Q000145:classification / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D017422:Sequence Analysis, DNA; D018454:Spouses; D055815:Young Adult