title: Oxidative damage and redox change in pea seeds treated with cadmium.
abstract: Pea seeds (Pisum sativum L.) were germinated by soaking in distilled water or 5mM CdCl2 for 5 days. The relationships among Cd treatment, germination rate, embryonic axis growth, NAD(P)H levels and NAD(P)H oxidase activities in mitochondrial and peroxisomal fractions of cotyledons and embryonic axis were investigated. Heavy metal stress provoked a diminution in germination percent and embryonic axis growth, as compared to the control. A drastic disorder in reducing power was imposed after exposure to cadmium. Heavy metal caused a significant increase in the redox ratio of coenzymes. NADPH oxidase is considered to be oxidative stress-related enzymes. The NAD(P)H oxidase activities were strongly stimulated after Cd exposure. The changes in redox and oxidative properties are discussed in relation to the delay in seed germination and embryonic axis growth.
authors.affiliation: Biophysiologie cellulaires, faculté des sciences de Bizerte, 7021 Zarzouna, Tunisia. moez.smiri@scbiol.uhp-nancy.fr
authors.forename: Moêz
authors.initials: M
authors.lastname: Smiri
authors.forename: Abdelilah
authors.initials: A
authors.lastname: Chaoui
authors.forename: Nicolas
authors.initials: N
authors.lastname: Rouhier
authors.forename: Eric
authors.initials: E
authors.lastname: Gelhaye
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Jacquot
authors.forename: Ezzedine
authors.initials: E
authors.lastname: El Ferjani
mesh_terms: D019256:Cadmium Chloride / Q000633:toxicity*; D018548:Cotyledon / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D018525:Germination / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D019255:NADPH Oxidases / Q000378:metabolism; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects*; D018532:Pisum sativum / Q000187:drug effects* / Q000378:metabolism*; D020675:Peroxisomes / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D012639:Seeds / Q000187:drug effects / Q000378:metabolism
chemical_list: D009249:NADP; D019255:NADPH Oxidases; D019256:Cadmium Chloride