title: CTLA-4 exon 1 +49 polymorphism alone and in a haplotype with -318 promoter polymorphism may confer susceptibility to chronic HBV infection in Chinese Han patients.
abstract: Cytotoxic T-lymphocyte-associated antigen 4 (CTLA-4) plays a pivotal role in regulating T cell activation, which is believably critical for the outcome of hepatitis B virus (HBV) infection. The expression and function of CTLA-4 may be affected by gene polymorphisms. This study investigated the influence of CTLA-4 polymorphisms on disease susceptibility in Chinese Han patients with chronic HBV infection. CTLA-4 +49A/G and -318C/T polymorphisms were evaluated by DNA amplification with polymerase chain reaction followed by the restriction fragment length polymorphism analysis. The patients with chronic HBV infection had higher frequencies of genotype AA and allele A of CTLA-4 +49A/G polymorphism. The haplotype +49A-318C was significantly over-represented (P < 0.001) and haplotype +49G-318C under-represented (P = 0.006) in the patients. The +49GG genotype was more frequent (P = 0.009) and +49A allele was less frequent in patients with lower ALT levels (P = 0.012) in HBeAg positive chronic hepatitis B. It is indicated that CTLA-4 +49A/G polymorphism alone and in a haplotype with -318C allele may confer susceptibility to chronic HBV infection in Chinese Han patients.
authors.affiliation: Department of Infectious Diseases, First Affiliated Hospital, School of Medicine, Xi'an Jiaotong University, Xi'an 710061, Shaanxi Province, People's Republic of China.
authors.forename: Shaoqiong
authors.initials: S
authors.lastname: Duan
authors.forename: Guoyu
authors.initials: G
authors.lastname: Zhang
authors.forename: Qunying
authors.initials: Q
authors.lastname: Han
authors.forename: Zhu
authors.initials: Z
authors.lastname: Li
authors.forename: Zhengwen
authors.initials: Z
authors.lastname: Liu
authors.forename: Jinghong
authors.initials: J
authors.lastname: Chen
authors.forename: Yi
authors.initials: Y
authors.lastname: Lv
authors.forename: Na
authors.initials: N
authors.lastname: Li
authors.forename: Yawen
authors.initials: Y
authors.lastname: Wang
authors.forename: Man
authors.initials: M
authors.lastname: Li
authors.forename: Sai
authors.initials: S
authors.lastname: Lou
authors.forename: Mingbo
authors.initials: M
authors.lastname: Yang
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Zhu
authors.forename: Fanfan
authors.initials: F
authors.lastname: Xing
mesh_terms: D044466:Asian People / Q000235:genetics*; D060908:CTLA-4 Antigen / Q000235:genetics*; D016022:Case-Control Studies; D002681:China; D005006:Ethnicity / Q000235:genetics; D005091:Exons / Q000235:genetics*; D005787:Gene Frequency / Q000235:genetics; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006239:Haplotypes / Q000235:genetics*; D006515:Hepatitis B virus / Q000502:physiology; D019694:Hepatitis B, Chronic / Q000235:genetics* / Q000821:virology; D006801:Humans; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011401:Promoter Regions, Genetic
chemical_list: D060908:CTLA-4 Antigen; C556706:CTLA4 protein, human