title: Expansion of human mesenchymal stem cells in a fixed-bed bioreactor system based on non-porous glass carrier - Part B: Modeling and scale-up of the system.
abstract: Human mesenchymal stem cells (hMSC) are a promising cell source for the manufacturing of cell therapy or tissue-engineered implants. In part A of this publication a fixed-bed bioreactor system based on non-porous borosilicate glass spheres and procedures for the automated expansion of hMSC with high yield and vitality was introduced. Part B of this study deals with the modeling of the process in order to transfer the bioreactor system from the laboratory to the production scale. Relevant model parameters were obtained by fitting them to the experimental data of hMSC-TERT cultivations in scales up to 300 cm3. Scale-up calculations were carried out exemplarily for a target cell number of twenty billion cells.
authors.affiliation: Institute of Biopharmaceutical Technology, University of Applied Sciences Giessen-Friedberg, Giessen, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Weber
authors.forename: Denise
authors.initials: D
authors.lastname: Freimark
authors.forename: Ralf
authors.initials: R
authors.lastname: Pörtner
authors.forename: Pablo
authors.initials: P
authors.lastname: Pino-Grace
authors.forename: Sebastian
authors.initials: S
authors.lastname: Pohl
authors.forename: Christine
authors.initials: C
authors.lastname: Wallrapp
authors.forename: Peter
authors.initials: P
authors.lastname: Geigle
authors.forename: Peter
authors.initials: P
authors.lastname: Czermak
mesh_terms: D019149:Bioreactors; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000295:instrumentation*; D002460:Cell Line; D049109:Cell Proliferation; D002470:Cell Survival; D003198:Computer Simulation; D004867:Equipment Design; D005898:Glass / Q000737:chemistry*; D005947:Glucose / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D059630:Mesenchymal Stem Cells / Q000378:metabolism / Q000502:physiology*; D008954:Models, Biological; D010101:Oxygen Consumption; D016062:Porosity; D013499:Surface Properties
chemical_list: D005947:Glucose