title: [Inhibitive effect of acanthopanax senticosus injection on gentamicin-induced ototoxicity in guinea pigs].
abstract: OBJECTIVE
  To study the antagonistic action of acanthopanax senticosus injection (ASS) on gentamicin ototoxicity.
  
  
  METHODS
  Guinea pigs were randomly divided into control group, GM group, ASS group, and ASS + GM group. The changes of hearing threshold, cochlear morphology, expression of caspases-3 were determined by ABR, TEM, and Western blot, respectively.
  
  
  RESULTS
  The ABR threshold in GM group increased markedly. There was no significant difference in ABR threshold between ASS group and control group, but the ABR threshold in ASS group was much lower than that both in GM group and ASS + GM group. Severely injured hair cells with morphological characteristics of apoptosis were found under TEM in GM group, and the hair cells were less injured in ASS + GM group. The results of Western blot showed that the expression of caspase-3 increased markedly in GM group, but it increased slightly in ASS + GM group.
  
  
  CONCLUSIONS
  ASS may antagonize the GM ototoxicity by inhibiting the expression of caspases-3.
authors.affiliation: Hearing Research Laboratory of Basic Medical Collage, China Medical University, Shenyang 110001, China.
authors.forename: Lin-Lin
authors.initials: LL
authors.lastname: Jia
authors.forename: Hao
authors.initials: H
authors.lastname: Tang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000378:metabolism; D028001:Eleutherococcus; D005839:Gentamicins / Q000633:toxicity*; D006168:Guinea Pigs; D006198:Hair Cells, Auditory / Q000166:cytology / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*
chemical_list: D005839:Gentamicins; D010936:Plant Extracts; D053148:Caspase 3