title: Spectrophotometric study of the interaction between chlorotetracycline and bovine serum albumin using Eosin Y as site marker with the aid of chemometrics.
abstract: Interaction of chlorotetracycline (CTC) with bovine serum albumin (BSA) was investigated under simulated physiological conditions by spectroscopy with the aid of multivariate curve resolution-alternating least squares (MCR-ALS). Eosin Y was selected as an alternative site I marker on the BSA to study the above molecular interaction. The binding of Eosin Y and CTC to BSA showed that CTC was displaced from CTC-BSA complex by Eosin Y, and Eosin Y-BSA complex was formed. However, the recorded fluorescence spectra of Eosin Y and Eosin Y-BSA overlapped and MCR-ALS was applied to resolve the two-way fluorescence spectra. From the resolved equilibrium concentration profiles, it was observed that Eosin Y competed with CTC in the binding process with BSA; it was also shown that the binding site of CTC on BSA was site I, and this was further confirmed by the fluorescence polarization method. Compared with some common site I markers for BSA, the fluorescence and UV-vis spectral shapes of the Eosin Y-BSA complex were quite different from that of Eosin Y, and this feature facilitated the investigation of the small molecule-BSA interaction.
authors.affiliation: Stake Laboratory of Food Science and Technology, Department of Chemistry, Nanchang University, Nanchang, Jiangxi 330031, China. ynni@ncu.edu.cn
authors.forename: Yongnian
authors.initials: Y
authors.lastname: Ni
authors.forename: Qiuhong
authors.initials: Q
authors.lastname: Liu
authors.forename: Serge
authors.initials: S
authors.lastname: Kokot
mesh_terms: D000818:Animals; D001665:Binding Sites; D001667:Binding, Competitive; D002417:Cattle; D002751:Chlortetracycline / Q000737:chemistry / Q000378:metabolism*; D004801:Eosine Yellowish-(YS) / Q000737:chemistry / Q000378:metabolism*; D016018:Least-Squares Analysis; D012710:Serum Albumin, Bovine / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet; D014859:Warfarin / Q000737:chemistry
chemical_list: D012710:Serum Albumin, Bovine; D014859:Warfarin; D004801:Eosine Yellowish-(YS); D002751:Chlortetracycline