title: [Expressions of Tau protein during the differentiation process of mesenchymal stem cells into neural cells].
abstract: OBJECTIVE
  To observe expressions and changes of Tau protein, pSer202 and Tau protein's contents during the differentiation process of bone-marrow mesenchymal stem cells (MSCs) into neural cells, and discuss Tau's effects on it.
  
  
  METHODS
  EGF and bFGF were combined for the induction of 4th, 8th, and 12th-MSCs into neural cells. Expressions of Tau protein and pSer202 were tested by immunocytochemistry. ELISA assay was applied for testing Tau protein's contents during differentiation process.
  
  
  RESULTS
  Positive rates of Tau protein in uninduced MSCs of 4th, 8th, and 12th-MSCs were under < 6%; After 14-day induction, the cellular morphologic characteristics in different passages were very similar to neurons, positive rates of Tau protein had no significant differences between passages (P > 0.05), but had differences with their uninduced groups (P < 0.05). There hadn't had expression of pSer202 in uninduced and induced groups of passages. ELISA assay indicated that there was an upward tendency in Tau protein's contents during the 14-day induction process, those in the 14th day had no significant differences between passages too (P > 0.05).
  
  
  CONCLUSIONS
  The increase in Tau protein's expressions and its non-phosphorylated state may make for MSCs differentiating into normal neural cells and formation of neuronal processes.
authors.affiliation: Department of Pathophysiology, Medical College of Zhengzhou University, Zhengzhou 450052, China.
authors.forename: Wen-Hai
authors.initials: WH
authors.lastname: Yan
authors.forename: Xuan-Hui
authors.initials: XH
authors.lastname: Xu
authors.forename: Yan
authors.initials: Y
authors.lastname: Xu
authors.forename: Xue-Fei
authors.initials: XF
authors.lastname: Han
authors.forename: Lan
authors.initials: L
authors.lastname: Ma
authors.forename: Jian-Zhi
authors.initials: JZ
authors.lastname: Wang
authors.forename: Ying
authors.initials: Y
authors.lastname: Xing
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D002454:Cell Differentiation; D002478:Cells, Cultured; D006168:Guinea Pigs; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D009474:Neurons / Q000166:cytology*; D016875:tau Proteins / Q000378:metabolism*
chemical_list: D016875:tau Proteins