title: Metabolomics-based identification of apoptosis-inducing metabolites in recombinant fed-batch CHO culture media.
abstract: A liquid chromatography-mass spectrometry (LC-MS) based metabolomics platform was previously established to identify and profile extracellular metabolites in culture media of mammalian cells. This presented an opportunity to isolate novel apoptosis-inducing metabolites accumulating in the media of antibody-producing Chinese hamster ovary (CHO mAb) fed-batch bioreactor cultures. Media from triplicate cultures were collected daily for the metabolomics analysis. Concurrently, cell pellets were obtained for determination of intracellular caspase activity. Metabolite profiles from the LC-MS data were subsequently examined for their degree of correlation with the caspase activity. A panel of extracellular metabolites, the majority of which were nucleotides/nucleosides and amino acid derivatives, exhibited good (R² > 0.8) and reproducible correlation. Some of these metabolites, such as oxidized glutathione, AMP and GMP, were later shown to induce apoptosis when introduced to fresh CHO mAb cultures. Finally, metabolic engineering targets were proposed to potentially counter the harmful effects of these metabolites.
authors.affiliation: Bioprocessing Technology Institute, A*STAR (Agency for Science, Technology and Research), 20 Biopolis Way, #06-01, Singapore 138668, Singapore. william chong@bti.a-star.edu.sg
authors.forename: William P K
authors.initials: WP
authors.lastname: Chong
authors.forename: Faraaz N K
authors.initials: FN
authors.lastname: Yusufi
authors.forename: Dong-Yup
authors.initials: DY
authors.lastname: Lee
authors.forename: Satty G
authors.initials: SG
authors.lastname: Reddy
authors.forename: Niki S C
authors.initials: NS
authors.lastname: Wong
authors.forename: Chew Kiat
authors.initials: CK
authors.lastname: Heng
authors.forename: Miranda G S
authors.initials: MG
authors.lastname: Yap
authors.forename: Ying Swan
authors.initials: YS
authors.lastname: Ho
mesh_terms: D000241:Adenosine / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D017209:Apoptosis; D051017:Apoptosis Regulatory Proteins / Q000737:chemistry*; D019149:Bioreactors; D016466:CHO Cells; D020169:Caspases / Q000378:metabolism; D002453:Cell Cycle; D002853:Chromatography, Liquid / Q000379:methods; D006224:Cricetinae; D003412:Cricetulus; D013058:Mass Spectrometry / Q000379:methods; D055432:Metabolomics; D011994:Recombinant Proteins / Q000737:chemistry*
chemical_list: D051017:Apoptosis Regulatory Proteins; D011994:Recombinant Proteins; D000255:Adenosine Triphosphate; D020169:Caspases; D000241:Adenosine