title: Effects of oligopeptide's conformational changes on its adsorption.
abstract: We report the effects of peptide adsorption to cross-linked polymers (adsorbents) by its conformational changes. Two adsorbents, APhe and ALeu, were prepared and expected to show high affinity to the oligopeptide VW-8 (NH(2)-Val-Val-Arg-Gly-Cys-Thr-Trp-Trp-COOH) according to our previous studies. These absorbents bared the residues of phenylalanine and leucine, respectively, and carried both hydrophobic and electrical groups. The adsorbent AAsp, which carried only the electrostatic groups, was also prepared as a reference. Both APhe and ALeu were found to exhibit higher VW-8 capacity than AAsp, in which APhe showed the highest VW-8 capacity (13.6 mg/g). The VW-8 adsorption to ALeu and APhe was analyzed using a variety of techniques, including the surface plasmon resonance (SPR) technology, nuclear magnetic resonance (NMR) spectra and isothermal titration calorimetry (ITC). The comprehensive experimental data together indicated that APhe could induce a conformational change of VW-8 from a random-coil to a β-strand structure due to its ability to provide the strong ring stacking and electrostatic interactions, which is believed to be responsible for its highest adsorption affinity (K(a)=2.59×10(7) M(-1)). In contrast, the hydrophobic interactions provided by ALeu were not strong enough to induce a VW-8 conformational change to a regular structure, and therefore it exhibited a relatively lower affinity to VW-8 (K(a)=6.23×10(5) M(-1)). The results presented in this work showed that peptide adsorption can be influenced by its conformational changes induced by suitable adsorbents via strong non-covalent interactions.
authors.affiliation: Key Laboratory of Functional Polymer Materials, Ministry of Education, College of Chemistry, Nankai University, Tianjin 300071, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Feng
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Ji-Hong
authors.initials: JH
authors.lastname: Li
authors.forename: Li-Xue
authors.initials: LX
authors.lastname: Fu
authors.forename: Jian-Xin
authors.initials: JX
authors.lastname: Zhao
authors.forename: Yi-Tao
authors.initials: YT
authors.lastname: Qiao
authors.forename: Ping-Chuan
authors.initials: PC
authors.lastname: Sun
authors.forename: Zhi
authors.initials: Z
authors.lastname: Yuan
mesh_terms: D000327:Adsorption; D015394:Molecular Structure; D009842:Oligopeptides / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D011487:Protein Conformation; D013499:Surface Properties
chemical_list: D009842:Oligopeptides; D011108:Polymers