title: [Effects of soy isoflavones on the expression of Bax mRNA and Ca(2+)-ATPase activity in ovaries of perimenopause rats].
abstract: OBJECTIVE
  To investigate the effects of soy isoflavones (SI) on the expression of Bax mRNA and Ca(2+) -ATPase activity in ovaries of perimenopause rats.
  
  
  METHODS
  The animal model of perimenopause rats was established by unforced aging. 12 month-old presenilins female Wistar rats were administered by intragastric (ig) with low (500 mg/kg), middle (158 mg/kg) and high (500 mg/kg) does of SI for 8 weeks. The expression of Bax mRNA in ovaries were detected by RT-PCR. Ca(2+) -ATPase activity in ovaries and MDA content and SOD activity in serum were detected by chemi-chromatometry.
  
  
  RESULTS
  Intervention of SI could significantly decrease the expression of Bax mRNA in ovaries and MDA content in serum, increase Ca(2+) -ATPase activity in ovaries and SOD activity in serum of presenilins rats (P < 0.05 or P < 0.01).
  
  
  CONCLUSIONS
  Soy isoflavones could down-regulate the expression of Bax mRNA and increase Ca(2+) -ATPase activity in aged ovaries. It is probably one of the mechanisms to improve the function of aged ovaries in perimenopause rats.
authors.affiliation: Department of Physiology, Harbin Medical University, Harbin 150086, China. whx20020920@163.com
authors.forename: Hai-Xia
authors.initials: HX
authors.lastname: Wen
authors.forename: Xiao-Hui
authors.initials: XH
authors.lastname: Xiao
authors.forename: Wei
authors.initials: W
authors.lastname: Zhao
authors.forename: Guo-Yi
authors.initials: GY
authors.lastname: Liu
authors.forename: Hong-Zhe
authors.initials: HZ
authors.lastname: Song
authors.forename: Jiang
authors.initials: J
authors.lastname: Ni
mesh_terms: D000818:Animals; D000252:Calcium-Transporting ATPases / Q000378:metabolism*; D005260:Female; D007529:Isoflavones / Q000494:pharmacology*; D010053:Ovary / Q000187:drug effects* / Q000378:metabolism; D047648:Perimenopause; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D013025:Glycine max / Q000737:chemistry; D051028:bcl-2-Associated X Protein / Q000378:metabolism*
chemical_list: C494828:Bax protein, rat; D007529:Isoflavones; D012333:RNA, Messenger; D051028:bcl-2-Associated X Protein; D000252:Calcium-Transporting ATPases