title: Minor histocompatibility antigens in Tunisians: could platelet endothelial cell adhesion molecule 1 marker be one of them?
abstract: Platelet endothelial cell adhesion molecule 1 (PECAM-1/CD31) is one of the human minor histocompatibility antigens that are the main targets of alloreactive T-cells after hematopoietic stem cells or solid organs transplantation. In order to investigate its polymorphism in Tunisians, three single nucleotide polymorphisms (SNPs) (rs668, rs12953 and rs1131012) were selected to perform an allele and haplotype analysis. Hundred-and-forty-two healthy and unrelated subjects were enrolled in this survey. Genomic DNAs were extracted using salting out method. SNP genotyping assays were performed with home-designed sequence-specific primers polymerase chain reaction (SSP-PCR). As a result, molecular analysis showed that PECAM-1 is one of the most polymorphic markers in the Tunisian population because minor allele frequency was 0.3, and minimum haplotype frequency was 0.03. A low linkage disequilibrium (D' = 0.45) between rs12953 and rs1131012 was noticed, although all other loci were in the Hardy-Weinberg equilibrium (minimum P value = 0.07). The frequencies were close to those reported in African-American and Caucasian groups.
authors.affiliation: Department of Immunohaematology, The National Blood Transfusion Center of Tunis, Tunis, Tunisia. sellamimh@laposte.net
authors.forename: M H
authors.initials: MH
authors.lastname: Sellami
authors.forename: H
authors.initials: H
authors.lastname: Kaabi
authors.forename: A
authors.initials: A
authors.lastname: Bibi
authors.forename: C
authors.initials: C
authors.lastname: Sahli
authors.forename: M
authors.initials: M
authors.lastname: Bani
authors.forename: A
authors.initials: A
authors.lastname: Ben Ahmed
authors.forename: T
authors.initials: T
authors.lastname: Massoud
authors.forename: S
authors.initials: S
authors.lastname: Hmida
mesh_terms: D015415:Biomarkers; D005787:Gene Frequency; D056726:Genetic Association Studies; D006801:Humans; D015778:Minor Histocompatibility Antigens / Q000235:genetics*; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D014416:Tunisia
chemical_list: D015415:Biomarkers; D015778:Minor Histocompatibility Antigens; D019408:Platelet Endothelial Cell Adhesion Molecule-1