title: Bead diffusion assay for discovering antimicrobial cyclic peptides.
abstract: A combinatorial library of 15,536 cyclic decapeptide analogues of tyrocidine A and gramicidin S was prepared on photocleavable TentaGel beads. The beads were photolyzed without solvent, and spread onto an agar plate inoculated with bacterial lawn. Clear zones were formed around beads carrying antimicrobially active peptides such as E18 c(kVOrnLfThiYOrnLq), which inhibited growth of B. subtilis and selected MRSA strains.
authors.affiliation: Department of Chemistry and Biochemistry, University of Berne, Freiestrasse 3, CH-3012 Berne, Switzerland.
authors.forename: Viviana S
authors.initials: VS
authors.lastname: Fluxà
authors.forename: Noélie
authors.initials: N
authors.lastname: Maillard
authors.forename: Malcolm G P
authors.initials: MG
authors.lastname: Page
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Reymond
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects*; D020650:Combinatorial Chemistry Techniques; D006096:Gramicidin / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D019151:Peptide Library; D010456:Peptides, Cyclic / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D014440:Tyrocidine / Q000737:chemistry*
chemical_list: D000890:Anti-Infective Agents; D019151:Peptide Library; D010456:Peptides, Cyclic; D014440:Tyrocidine; D006096:Gramicidin