title: MIH supplementation strategies: prospective clinical and laboratory trial.
abstract: The use of calcium-phosphate casein on hypomineralized molars (molar incisor hypomineralization, MIH) has been proposed but not clinically investigated. Qualitative and quantitative effects of supplementation with a calcium-phosphate casein product on MIH molars were monitored over a period of three years. Molar replicas, minimally invasive biopsies and their SEM microphotographs, plus ESEM/EDX semi-quantitative peaks of elements present in affected enamel were evaluated. Mineralization, morphology, and porosity appeared markedly improved, with calcium and phosphate levels reaching almost normal levels at three years' follow-up. The hypothesis tested was rejected, since calcium-phosphate casein improved enamel morphology in vivo.
authors.affiliation: Department of Oral Sciences, Section of Endo-Pedodontics, Alma Mater Studiorum, University of Bologna, Via San Vitale, 59-40125 Bologna, Italy. chiara.baroni@unibo.it
authors.forename: C
authors.initials: C
authors.lastname: Baroni
authors.forename: S
authors.initials: S
authors.lastname: Marchionni
mesh_terms: D000287:Administration, Topical; D002364:Caseins / Q000008:administration & dosage / Q000627:therapeutic use*; D002648:Child; D003743:Dental Enamel / Q000473:pathology* / Q000648:ultrastructure; D003744:Dental Enamel Hypoplasia / Q000188:drug therapy*; D006801:Humans; D008855:Microscopy, Electron, Scanning; D008903:Minerals / Q000032:analysis; D011446:Prospective Studies; D016628:Replica Techniques; D013052:Spectrometry, X-Ray Emission
chemical_list: D002364:Caseins; D008903:Minerals; C447032:casein phosphopeptide-amorphous calcium phosphate nanocomplex