title: Diversity of bacterial endophytes in roots of Mexican husk tomato plants (Physalis ixocarpa) and their detection in the rhizosphere.
abstract: Endophytic bacterial diversity was estimated in Mexican husk tomato plant roots by amplified rDNA restriction analysis and sequence homology comparison of the 16S rDNA genes. Sixteen operational taxonomic units from the 16S rDNA root library were identified based on sequence analysis, including the classes Gammaproteobacteria, Betaproteobacteria, Actinobacteria, and Bacilli. The predominant genera were Stenotrophomonas (21.9%), Microbacterium (17.1%), Burkholderia (14.3%), Bacillus (14.3%), and Pseudomonas (10.5%). In a 16S rDNA gene library of the same plant species' rhizosphere, only common soil bacteria, including Stenotrophomonas, Burkholderia, Bacillus, and Pseudomonas, were detected. We suggest that the endophytic bacterial diversity within the roots of Mexican husk tomato plants is a subset of the rhizosphere bacterial population, dominated by a few genera.
authors.affiliation: Laboratorio de Recombinacion y Diversidad Genomica, Instituto de Investigaciones Quimico Biologicas, Universidad Michoacana de San Nicolas de Hidalgo Morelia, Michoacan, Mexico.
authors.forename: H A
authors.initials: HA
authors.lastname: Marquez-Santacruz
authors.forename: R
authors.initials: R
authors.lastname: Hernandez-Leon
authors.forename: M C
authors.initials: MC
authors.lastname: Orozco-Mosqueda
authors.forename: I
authors.initials: I
authors.lastname: Velazquez-Sepulveda
authors.forename: G
authors.initials: G
authors.lastname: Santoyo
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000235:genetics; D001483:Base Sequence; D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D018551:Solanum lycopersicum / Q000235:genetics / Q000382:microbiology*; D018517:Plant Roots / Q000235:genetics / Q000382:microbiology*; D016133:Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial; D004275:DNA, Ribosomal