title: [Effect of cerebellar interposed nuclei on lymphocyte function].
abstract: OBJECTIVE
  To increase the cognition of cerebellar functions and the knowledge of neuroimmunology, the effect of cerebellar interposed nuclei (IN), one of three deep nuclei in cerebellum, on lymphocyte function was investigated.
  
  
  METHODS
  Kainic acid (KA) was microinjected into bilateral IN for lesions of neuronal bodies in the IN. Control rats was microinjected with saline into their IN. On days 8, 16 and 32 following the IN lesions, the lymphocyte number in the peripheral blood was measured by blood corpuscle counter. Meanwhile, lymphocyte proliferation induced by concanavalin A (Con A), cytotoxicity of natural killer (NK) cells against YAC-1 cells, and anti-SRBC IgM antibody in the serum were examined respectively by methyl-thiazole-tetrazolium (MTT) assay, flow cytometry and ELISA assay.
  
  
  RESULTS
  The lymphocyte number in the peripheral blood was significantly reduced on days 8, 16 and 32 following the effective lesions of the bilateral IN in comparison with that of control. The Con A-induced lymphocyte proliferation, the NK cell cytotoxicity to YAC-1 cells, and the titer of anti-SRBC IgM antibody in the serum, were all significantly attenuated on days 8, 16 and 32 following the effective lesions of the bilateral IN in comparison with those of control. There were not remarkable differences between the days 8, 16 and 32 in the decreased lymphocyte number and functions induced by the lesions of the bilateral IN.
  
  
  CONCLUSIONS
  Effective lesions of the cerebellar bilateral IN of rats cause an inhibition in lymphocyte number and functions of T, B and NK cells, strongly showing that the cerebellar IN can modulate lymphocyte functions.
authors.affiliation: Department of Physiology, School of Medicine, Nantong University, Nantong 226001, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Qiu
authors.forename: Yu-Ping
authors.initials: YP
authors.lastname: Peng
authors.forename: Yi-Hua
authors.initials: YH
authors.lastname: Qiu
mesh_terms: D000818:Animals; D002529:Cerebellar Nuclei / Q000276:immunology / Q000502:physiology*; D002531:Cerebellum / Q000276:immunology / Q000502:physiology*; D005260:Female; D007608:Kainic Acid; D007694:Killer Cells, Natural / Q000276:immunology; D018655:Lymphocyte Count; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D008845:Microinjections; D015213:Neuroimmunomodulation / Q000276:immunology*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D007608:Kainic Acid