title: Development of a rapid LC-MS/MS method for ribavirin determination in rat brain.
abstract: A rapid and specific liquid chromatography tandem mass spectrometry (LC-MS/MS) method for the determination of ribavirin (RBV) in rat brain was developed. Sample preparation required only two centrifuge steps before LC-MS/MS analysis and the chromatographic separation was achieved in isocratic conditions using an Atlantis T3 column with a nearly totally aqueous (95%) mobile phase. The method showed a good linearity over a concentration range of 5-1000ppb and satisfactory results in terms of accuracy.
authors.affiliation: Department of Veterinary Public Health and Animal Pathology, Alma Mater Studiorum, University of Bologna, via Tolara di Sopra 50, Ozzano dell'Emilia, Bologna, Italy.
authors.forename: Elisa
authors.initials: E
authors.lastname: Zironi
authors.forename: Teresa
authors.initials: T
authors.lastname: Gazzotti
authors.forename: Barbara
authors.initials: B
authors.lastname: Lugoboni
authors.forename: Andrea
authors.initials: A
authors.lastname: Barbarossa
authors.forename: Alessandra
authors.initials: A
authors.lastname: Scagliarini
authors.forename: Giampiero
authors.initials: G
authors.lastname: Pagliuca
mesh_terms: D053000:Analytic Sample Preparation Methods; D000818:Animals; D000998:Antiviral Agents / Q000032:analysis* / Q000737:chemistry; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid; D004355:Drug Stability; D057230:Limit of Detection; D008832:Microchemistry / Q000379:methods; D051381:Rats; D015203:Reproducibility of Results; D012254:Ribavirin / Q000032:analysis* / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
chemical_list: D000998:Antiviral Agents; D012254:Ribavirin