title: Physiological responses to folate overproduction in Lactobacillus plantarum WCFS1.
abstract: BACKGROUND
  Using a functional genomics approach we addressed the impact of folate overproduction on metabolite formation and gene expression in Lactobacillus plantarum WCFS1. We focused specifically on the mechanism that reduces growth rates in folate-overproducing cells.
  
  
  RESULTS
  Metabolite formation and gene expression were determined in a folate-overproducing- and wild-type strain. Differential metabolomics analysis of intracellular metabolite pools indicated that the pool sizes of 18 metabolites differed significantly between these strains. The gene expression profile was determined for both strains in pH-regulated chemostat culture and batch culture. Apart from the expected overexpression of the 6 genes of the folate gene cluster, no other genes were found to be differentially expressed both in continuous and batch cultures. The discrepancy between the low transcriptome and metabolome response and the 25% growth rate reduction of the folate overproducing strain was further investigated. Folate production per se could be ruled out as a contributing factor, since in the absence of folate production the growth rate of the overproducer was also reduced by 25%. The higher metabolic costs for DNA and RNA biosynthesis in the folate overproducing strain were also ruled out. However, it was demonstrated that folate-specific mRNAs and proteins constitute 8% and 4% of the total mRNA and protein pool, respectively.
  
  
  CONCLUSIONS
  Folate overproduction leads to very little change in metabolite levels or overall transcript profile, while at the same time the growth rate is reduced drastically. This shows that Lactobacillus plantarum WCFS1 is unable to respond to this growth rate reduction, most likely because the growth-related transcripts and proteins are diluted by the enormous amount of gratuitous folate-related transcripts and proteins.
authors.affiliation: TI Food & Nutrition, Wageningen, Nieuwe Kanaal 9A, 6709 PA, Wageningen, The Netherlands.
authors.forename: Arno
authors.initials: A
authors.lastname: Wegkamp
authors.forename: Astrid E
authors.initials: AE
authors.lastname: Mars
authors.forename: Magda
authors.initials: M
authors.lastname: Faijes
authors.forename: Douwe
authors.initials: D
authors.lastname: Molenaar
authors.forename: Ric C H
authors.initials: RC
authors.lastname: de Vos
authors.forename: Sebastian M J
authors.initials: SM
authors.lastname: Klaus
authors.forename: Andrew D
authors.initials: AD
authors.lastname: Hanson
authors.forename: Willem M
authors.initials: WM
authors.lastname: de Vos
authors.forename: Eddy J
authors.initials: EJ
authors.lastname: Smid
mesh_terms: D005492:Folic Acid / Q000096:biosynthesis* / Q000235:genetics; D020869:Gene Expression Profiling; D006160:Guanosine Triphosphate / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D048191:Lactiplantibacillus plantarum / Q000254:growth & development / Q000378:metabolism*; D055442:Metabolome; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D006160:Guanosine Triphosphate; D005492:Folic Acid