title: Molecular characterization of severe and mild cases of influenza A (H1N1) 2009 strain from Argentina.
abstract: While worldwide pandemic influenza A(H1N1) pdm case fatality rate (CFR) was 0.4%, Argentina's was 4.5%. A total of 34 strains from mild and severe cases were analyzed. A full genome sequencing was carried out on 26 of these, and a partial sequencing on the remaining eight. We observed no evidence that the high CFR can be attributed to direct virus changes. No evidence of re-assortment, mutations associated with resistance to antiviral drugs, or genetic drift that might contribute to virulence was observed. Although the mutation D225G associated with severity in the latest reports from the Ukraine and Norway is not observed among the Argentine strains, an amino acid change in the area (S206T) surrounding the HA receptor binding domain was observed, the same previously established worldwide.
authors.affiliation: Instituto Nacional de Enfermedades Infecciosas, ANLIS Dr. Carlos G. Malbrán, Buenos Aires, Argentina. ebaumeister@anlis.gov.ar
authors.forename: Elsa
authors.initials: E
authors.lastname: Baumeister
authors.forename: Gustavo
authors.initials: G
authors.lastname: Palacios
authors.forename: Daniel
authors.initials: D
authors.lastname: Cisterna
authors.forename: Alexander
authors.initials: A
authors.lastname: Solovyov
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Hui
authors.forename: Nazir
authors.initials: N
authors.lastname: Savji
authors.forename: Ana Valeria
authors.initials: AV
authors.lastname: Bussetti
authors.forename: Ana
authors.initials: A
authors.lastname: Campos
authors.forename: Andrea
authors.initials: A
authors.lastname: Pontoriero
authors.forename: Omar J
authors.initials: OJ
authors.lastname: Jabado
authors.forename: Craig
authors.initials: C
authors.lastname: Street
authors.forename: David L
authors.initials: DL
authors.lastname: Hirschberg
authors.forename: Raul
authors.initials: R
authors.lastname: Rabadan
authors.forename: Virginia
authors.initials: V
authors.lastname: Alonio
authors.forename: Viviana
authors.initials: V
authors.lastname: Molina
authors.forename: Stephen
authors.initials: S
authors.lastname: Hutchison
authors.forename: Michael
authors.initials: M
authors.lastname: Egholm
authors.forename: W Ian
authors.initials: WI
authors.lastname: Lipkin
mesh_terms: D000293:Adolescent; D000328:Adult; D001118:Argentina / Q000453:epidemiology; D002648:Child; D002675:Child, Preschool; D016000:Cluster Analysis; D004279:DNA, Viral / Q000235:genetics*; D005260:Female; D006801:Humans; D007223:Infant; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics* / Q000302:isolation & purification; D007251:Influenza, Human / Q000401:mortality / Q000821:virology*; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics; D011991:Receptors, Virus / Q000235:genetics; D015203:Reproducibility of Results; D012720:Severity of Illness Index; D055815:Young Adult
chemical_list: D004279:DNA, Viral; D012367:RNA, Viral; D011991:Receptors, Virus