title: [Expression profiling of UBF, a novel member of UBC family].
abstract: From large-scale sequence of human fetal liver cDNA library, we have obtained a full-length cDNA from an EST after further sequencing. It has been demonstrated by the alignment comparison with data base available that it is a novel member of Ubc family and got the number from GeneBank: UBF-F1 AF 294842.
  
  
  OBJECTIVE
  To demonstrate its authenticity, UBF was amplified from the total RNA of human fetal liver and HL-60 cell line using RT-PCR, and the PCR products were further sequenced and compared with the original UBF sequence. To evaluate the expression level and subcellular location of UBF in human multiple tissues, in situ hybridization was carried out on the frozen section of human fetal multiple tissues and HL-60 cell line with DIG-labeled UBF cDNA probes.
  
  
  RESULTS
  The experimental results of RT-PCR and sequencing showed that the sequence of RT-PCR products were the same as the original UBF. The experimental results of in situ hybridization showed that UBF was expressed widely by human multiple fetal tissues and the expression level were very high in HL-60 cells.
  
  
  CONCLUSIONS
  It is suggested that the special structure of UBF is authentic, and the expression profiling research of UBF shows that UBF is expressed widely by human multiple fetal tissues and the expression level is very high in HL-60 cells, implying that UBF plays the important function in the developing tissues and leukemia cells. It is also suggested that UBF may be functionally related with the nucleic-involving cellular activities based on the results of sub-cellular localizations.
authors.affiliation: Department of Genomics and Proteomics, Institute of Radiation Medicine, Beijing 100850, China.
authors.forename: Chun-Ping
authors.initials: CP
authors.lastname: Cui
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yong-Tao
authors.initials: YT
authors.lastname: Yu
authors.forename: Yu-Zhen
authors.initials: YZ
authors.lastname: Li
authors.forename: Yu
authors.initials: Y
authors.lastname: Geng
authors.forename: Shi-Fu
authors.initials: SF
authors.lastname: Zhao
mesh_terms: D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D020869:Gene Expression Profiling; D018922:HL-60 Cells; D006801:Humans; D008969:Molecular Sequence Data; D035463:Pol1 Transcription Initiation Complex Proteins / Q000235:genetics*; D016364:Reading Frames; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D044763:Ubiquitin-Conjugating Enzymes / Q000145:classification*; D054875:Ubiquitination
chemical_list: D035463:Pol1 Transcription Initiation Complex Proteins; C063844:transcription factor UBF; D044763:Ubiquitin-Conjugating Enzymes