title: The carboxy-terminal region of SMAP2 directs subcellular localization as well as Arf protein specificity.
abstract: Small G proteins play a central role in the organization of secretory and endocytotic pathways. The recruitment of some effectors, including vesicle coat proteins, is mediated by the ADP-ribosylation factor (Arf) family. Arf proteins have distinct subcellular localizations. ArfGAPs (Arf GTPase-activating proteins) regulate Arf GTPase activity. Thus, each ArfGAP is distinctly localized to allow it to maintain a specific interaction with its target Arf(s). However, the domains that regulate the subcellular localization of ArfGAPs and the way in which these subcellular localizations affect the target specificities of ArfGAPs remain unclear. Recently, we identified two novel ArfGAPs, SMAP1 (Small ArfGAP protein 1) and SMAP2. In the current study, we identified sequences in the carboxy-terminal region of SMAP2 that are critical for its specific subcellular localization and its specificity for Arf proteins.
authors.affiliation: Department of Biological Science, Graduate School of Humanities and Sciences, Nara Women's University, Kitauoyanishi-machi, Nara 630-8506, Japan.
authors.forename: Ikuko
authors.initials: I
authors.lastname: Sakakura
authors.forename: Kenji
authors.initials: K
authors.lastname: Tanabe
authors.forename: Natsumi
authors.initials: N
authors.lastname: Nouki
authors.forename: Mai
authors.initials: M
authors.lastname: Suzuki
authors.forename: Masanobu
authors.initials: M
authors.lastname: Satake
authors.forename: Toshio
authors.initials: T
authors.lastname: Watanabe
mesh_terms: D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D020690:GTPase-Activating Proteins / Q000235:genetics / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D042541:Intracellular Space / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D020690:GTPase-Activating Proteins; D008565:Membrane Proteins; D011993:Recombinant Fusion Proteins; C107022:SMAP1 protein, human; C553893:SMAP2 protein, human