title: Analysis of epitopes in the capsid protein of avian hepatitis E virus by using monoclonal antibodies.
abstract: Avian hepatitis E virus (HEV) is related genetically and antigenically to human and swine HEVs and capsid protein of avian HEV shares approximately 48-49% amino acid sequence identities with those of human and swine HEVs. Six monoclonal antibodies (MAbs) were produced and used to locate different epitopes in the ORF2 region of aa 339-570 of avian HEV Chinese isolate. The results showed that five epitopes were located in the aa 339-414 region and one in the aa 510-515 region. Two epitopes located in aa 339-355 and aa 384-414 regions are the immunodominant epitopes on the surface of the avian HEV particles as demonstrated by immune capture of viral particles and immunohistochemical detection of the ORF2 antigens with two MAbs.
authors.affiliation: Department of Preventive Veterinary Medicine, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai An, Shandong 271018, China.
authors.forename: Shiwei
authors.initials: S
authors.lastname: Dong
authors.forename: Qin
authors.initials: Q
authors.lastname: Zhao
authors.forename: Mingzhe
authors.initials: M
authors.lastname: Lu
authors.forename: Peiming
authors.initials: P
authors.lastname: Sun
authors.forename: Hongkai
authors.initials: H
authors.lastname: Qiu
authors.forename: Lu
authors.initials: L
authors.lastname: Zhang
authors.forename: Junhua
authors.initials: J
authors.lastname: Lv
authors.forename: En-Min
authors.initials: EM
authors.lastname: Zhou
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification; D000914:Antibodies, Viral / Q000276:immunology* / Q000302:isolation & purification; D036022:Capsid Proteins / Q000276:immunology*; D002645:Chickens; D002681:China; D006524:Hepatitis, Viral, Animal / Q000821:virology; D052678:Hepevirus / Q000276:immunology* / Q000302:isolation & purification; D016056:Immunodominant Epitopes / Q000276:immunology*; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D008853:Microscopy; D011201:Poultry Diseases / Q000821:virology; D012327:RNA Virus Infections / Q000821:virology
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D036022:Capsid Proteins; D016056:Immunodominant Epitopes