title: [Study on the relationship of metallothionein and antioxidases in protective mechanism of cardiomyocytes].
abstract: OBJECTIVE
  To study the relationship of Metallothionein (MT) and antioxidases in hypoxia/reoxygenation (H/R) and hypoxic preconditioning (HPC).
  
  
  METHODS
  H/R and HPC model of rat cultured cardiomyocytes were established. Then the contents of MT and relevant changes of activities of antioxidases (SOD, CAT, GSHpx) of cardiomyocytes were determined while pretreated with the treatment: HPC. Zn2+ inducing MT or MT antibody.
  
  
  RESULTS
  In HPC and Zn2+ induced groups, all the contents of MT and the activities of antioxidases were obviously higher than those in H/R (P < 0.05, P < 0.01 respectively). While using MT, the activities of SOD were progressively increased than control (P < 0.05), CAT and GSHpx activities were lower than control (P < 0.01), but were significantly higher than those in I/R group (P < 0.01). In MT antibody groups, the activities of antioxidase were decreased extremely.
  
  
  CONCLUSIONS
  MT has effects on the cardiomyocytes protective role of HPC. And can afford more capacity for cardiomyocytes to tolerate the H/R damage by the mechanism of increasing the activities of antioxidase.
authors.affiliation: Institute of Military Medicine of Nanjing Command PLA, Nanjing 210002.
authors.forename: H Y
authors.initials: HY
authors.lastname: Jin
authors.forename: F Q
authors.initials: FQ
authors.lastname: Li
authors.forename: D X
authors.initials: DX
authors.lastname: Fang
authors.forename: S Q
authors.initials: SQ
authors.lastname: Li
authors.forename: W G
authors.initials: WG
authors.lastname: Tan
authors.forename: H B
authors.initials: HB
authors.lastname: Chen
mesh_terms: D000818:Animals; D002374:Catalase / Q000378:metabolism; D015687:Cell Hypoxia; D002478:Cells, Cultured; D005979:Glutathione Peroxidase / Q000378:metabolism; D019194:Ischemic Preconditioning; D008668:Metallothionein / Q000378:metabolism*; D032383:Myocytes, Cardiac / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D008668:Metallothionein; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase