title: Phylogenetic identification of Cystoisospora spp. from dogs, cats, and raccoon dogs in Japan.
abstract: Cystoisospora spp. from feces in dogs, cats, and raccoon dogs were isolated, sequenced at the small subunit ribosomal RNA gene locus and compared to other Cystoisospora spp. Cystoisospora oocysts from dogs and raccoon dogs were morphologically similar with those of C. ohioensis, and cat isolates were similar with those of C. felis. The sequences from dogs and raccoon dogs, and cats have a homology with C. ohioensis and C. felis, respectively. Phylogenetic analysis of the DNA sequences showed that the dog and raccoon dog isolates were nested in a clade with other Cystoisospora spp. including C. ohioensis, C. belli, and C. orlovi. The cat isolate formed a sister group with C. felis that was a separate clade from the dog and raccoon dog group. We report sequence variation in these Cystoisospora sequences and have identified raccoon dogs as another carnivore host for Cystoisospora spp. infecting dogs.
authors.affiliation: National Institute of Animal Health, National Agriculture and Food Research Organization, Tsukuba, Ibaraki 305-0856, Japan. matsubayashi@affrc.go.jp
authors.forename: Makoto
authors.initials: M
authors.lastname: Matsubayashi
authors.forename: Ramon A
authors.initials: RA
authors.lastname: Carreno
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Tani
authors.forename: Ryusaku
authors.initials: R
authors.lastname: Yoshiuchi
authors.forename: Takenori
authors.initials: T
authors.lastname: Kanai
authors.forename: Isao
authors.initials: I
authors.lastname: Kimata
authors.forename: Shigehiko
authors.initials: S
authors.lastname: Uni
authors.forename: Masaru
authors.initials: M
authors.lastname: Furuya
authors.forename: Kazumi
authors.initials: K
authors.lastname: Sasai
mesh_terms: D000818:Animals; D002371:Cat Diseases / Q000453:epidemiology / Q000469:parasitology*; D002415:Cats; D003048:Coccidiosis / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D004283:Dog Diseases / Q000453:epidemiology / Q000469:parasitology*; D004285:Dogs; D021743:Eimeriidae / Q000235:genetics*; D005243:Feces / Q000469:parasitology; D007564:Japan / Q000453:epidemiology; D010802:Phylogeny; D046031:Raccoon Dogs