title: Structure of the O-polysaccharide and characterization of the O-antigen gene cluster of Salmonella enterica O53.
abstract: The O-polysaccharide (O-antigen) of Salmonella enterica O53 was isolated by mild acid degradation of the lipopolysaccharide and studied by sugar analysis and (1)H and (13)C NMR spectroscopy before and after O-deacetylation. The following structure of the tetrasaccharide O-unit was established: Sequencing of the O-antigen gene cluster of S. enterica O53 revealed 11 open reading frames having the same transcriptional direction. The functions were tentatively assigned to the genes based on their similarity to related genes from the available databases and taking into account the S. enterica O53-polysaccharide structure.
authors.affiliation: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation. perepel@ioc.ac.ru
authors.forename: Andrei V
authors.initials: AV
authors.lastname: Perepelov
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
authors.forename: Sof'ya N
authors.initials: SN
authors.lastname: Senchenkova
authors.forename: Alexander S
authors.initials: AS
authors.lastname: Shashkov
authors.forename: Lu
authors.initials: L
authors.lastname: Feng
authors.forename: Yuriy A
authors.initials: YA
authors.lastname: Knirel
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
mesh_terms: D002240:Carbohydrate Sequence; D008969:Molecular Sequence Data; D005810:Multigene Family; D019081:O Antigens / Q000737:chemistry* / Q000235:genetics*; D019779:Salmonella enterica / Q000235:genetics* / Q000276:immunology*
chemical_list: D019081:O Antigens