title: Effect of human neural precursor cell transplantation on endogenous neurogenesis after focal cerebral ischemia in the rat.
abstract: Little is known about the relationship between neuronal cell transplantation and endogenous neurogenesis after experimental stroke. We found previously that transplantation of neuronal precursors derived from BG01 human embryonic stem cells reduced infarct volume and improved behavioral outcome after distal middle cerebral artery occlusion (MCAO) in rats. In this study, transplantation was performed 14 days after distal MCAO and doublecortin (Dcx)-expressing cells in the subventricular zone (SVZ) and subgranular zone of dentate gyrus (SGZ) were counted 60 days post-transplant. Transplantation increased neurogenesis (Dcx expression) in ipsilateral SVZ, but not in contralateral SVZ or either SGZ, in both young adult (3-month-old) and aged (24-month-old) rats. These findings suggest that cell-based therapy for stroke may be associated with changes in endogenous adaptive processes, including neurogenesis.
authors.affiliation: Buck Institute for Age Research, 8001 Redwood Blvd., Novato, CA 94945, USA.
authors.forename: Kunlin
authors.initials: K
authors.lastname: Jin
authors.forename: Lin
authors.initials: L
authors.lastname: Xie
authors.forename: XiaoOu
authors.initials: X
authors.lastname: Mao
authors.forename: Maeve B
authors.initials: MB
authors.lastname: Greenberg
authors.forename: Alexander
authors.initials: A
authors.lastname: Moore
authors.forename: Botao
authors.initials: B
authors.lastname: Peng
authors.forename: Rose B
authors.initials: RB
authors.lastname: Greenberg
authors.forename: David A
authors.initials: DA
authors.lastname: Greenberg
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000473:pathology / Q000601:surgery*; D002478:Cells, Cultured; D000089662:Doublecortin Protein; D053595:Embryonic Stem Cells / Q000502:physiology / Q000637:transplantation*; D006801:Humans; D008297:Male; D055495:Neurogenesis / Q000502:physiology*; D009474:Neurons / Q000502:physiology / Q000637:transplantation*; D051381:Rats; D011916:Rats, Inbred F344; D033581:Stem Cell Transplantation / Q000379:methods