title: CCR10 and CCL27 are overexpressed in cutaneous squamous cell carcinoma.
abstract: C-C chemokine receptor (CCR)10 is a specific receptor for chemokine ligand (CCL)27, a selective chemoattractant for skin-associated memory T cells to cutaneous sites. In melanoma, CCR10 increases the ability of neoplastic cells to grow, invade tissues, disseminate to lymph nodes, and escape the host immune responses. In this study, we investigated the expression of CCR10 and its ligand CCL27 in squamous cell carcinoma (SCC). CCR10 and CCL27 were expressed in SCC, actinic keratosis (AK), Bowen's disease, and seborrheic keratosis (predominantly prickle cell type), but not in seborrheic keratosis (predominantly basal cell type) and basal cell carcinoma. Furthermore, CCR10 and CCL27 were overexpressed in SCC relative to Bowen's disease, an early stage of SCC. Consistently, a human SCC cell line, A253 cells, and HaCaT cells exhibited CCL27 production that was strongly induced by tumor necrosis factor-α and interleukin-1β. Finally, A253 cells expressed stronger intracellular CCR10 compared to HaCaT cells by flow cytometry. These results suggest that CCR10 and CCL27 overexpression in SCC is related to the progression of SCC and is useful for the diagnosis of SCC.
authors.affiliation: Department of Dermatology, Faculty of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan.
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Kai
authors.forename: Takafumi
authors.initials: T
authors.lastname: Kadono
authors.forename: Takashi
authors.initials: T
authors.lastname: Kakinuma
authors.forename: Manabu
authors.initials: M
authors.lastname: Tomita
authors.forename: Hanako
authors.initials: H
authors.lastname: Ohmatsu
authors.forename: Yoshihide
authors.initials: Y
authors.lastname: Asano
authors.forename: Yayoi
authors.initials: Y
authors.lastname: Tada
authors.forename: Makoto
authors.initials: M
authors.lastname: Sugaya
authors.forename: Shinichi
authors.initials: S
authors.lastname: Sato
mesh_terms: D001706:Biopsy; D001913:Bowen's Disease / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D002280:Basal Cell Carcinoma / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D002294:Carcinoma, Squamous Cell / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D054425:Chemokine CCL27 / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D053583:Interleukin-1beta / Q000276:immunology / Q000378:metabolism; D055623:Keratosis, Actinic / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D017492:Keratosis, Seborrheic / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D054447:Receptors, CCR10 / Q000378:metabolism*; D012867:Skin / Q000378:metabolism / Q000473:pathology; D012878:Skin Neoplasms / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology / Q000378:metabolism
chemical_list: C492276:CCL27 protein, human; D054425:Chemokine CCL27; D053583:Interleukin-1beta; D054447:Receptors, CCR10; D014409:Tumor Necrosis Factor-alpha