title: Genetic analysis of the 10 ChrX STRs loci in Chinese Han nationality from Guangdong province.
abstract: This study is to explore the polymorphic nature of X-Chromosome short tandem repeats (ChrX STRs) loci, and to determine its application in kinship tests for forensic cases. A new fluorescent multiplex PCR that simultaneously amplifies the 10 ChX STRs loci in the same PCR reaction had been set up. DXS7132, DXS981, DXS6801, DXS6809, DXS6789, DXS7424, DXS101, DXS7133, GATA165B12 and GATA31E08 were analyzed in a sample of 511 (399 males and 112 females) unrelated individuals from Guangdong Han nationality in China. One hundred and one alleles were observed in all the loci. Here, we investigated the allele frequencies and mutation rates of the ten loci, and then made the comparison of allele frequencies distribution among different populations. The results show the ten loci in the multiplex systems may provide high polymorphism information for kinship testing and relationship investigations, and it is necessary to gain allele frequency and mutation rate of different population for forensic application.
authors.affiliation: Faculty of Forensic Medicine, Zhongshan School of Medicine, Sun Yat-Sen University, 74, Zhongshan 2nd Road, Guangzhou 510080, People's Republic of China.
authors.forename: Qiu-Ling
authors.initials: QL
authors.lastname: Liu
authors.forename: De-Jian
authors.initials: DJ
authors.lastname: Lu
authors.forename: Wei-Wei
authors.initials: WW
authors.lastname: Wu
authors.forename: Hong-Lei
authors.initials: HL
authors.lastname: Hao
authors.forename: Ye-Fei
authors.initials: YF
authors.lastname: Chen
authors.forename: Hu
authors.initials: H
authors.lastname: Zhao
mesh_terms: D044466:Asian People / Q000235:genetics*; D002681:China; D041321:Chromosomes, Human, X / Q000235:genetics*; D005006:Ethnicity / Q000235:genetics*; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D056426:Genetic Loci / Q000235:genetics*; D005828:Genetics, Population; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*; D009154:Mutation / Q000235:genetics; D010375:Pedigree