title: Functional analysis of the twin-arginine translocation pathway in Sodalis glossinidius, a bacterial symbiont of the tsetse fly.
abstract: This study demonstrates a functional twin-arginine (Tat) translocation pathway present in the tsetse fly symbiont Sodalis glossinidius and its potential to export active heterologous proteins to the periplasm. Functionality was demonstrated using green fluorescent protein (GFP) fused to the Tat signal peptide of Escherichia coli trimethylamine N-oxide reductase (TorA).
authors.affiliation: Department of Parasitology, Unit of Entomology, Institute of Tropical Medicine Antwerp, Antwerp, Belgium.
authors.forename: Linda
authors.initials: L
authors.lastname: De Vooght
authors.forename: Guy
authors.initials: G
authors.lastname: Caljon
authors.forename: Marc
authors.initials: M
authors.lastname: Coosemans
authors.forename: Jan
authors.initials: J
authors.lastname: Van den Abbeele
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D004755:Enterobacteriaceae / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D010089:Oxidoreductases, N-Demethylating / Q000235:genetics / Q000378:metabolism; D013559:Symbiosis; D014370:Tsetse Flies / Q000382:microbiology*
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D026901:Membrane Transport Proteins; C408585:twin-arginine translocase complex, E coli; D049452:Green Fluorescent Proteins; D010089:Oxidoreductases, N-Demethylating; C023186:trimethylamine dehydrogenase