title: Non-specific and specific interactions on functionalized polymer surface studied by FT-SPR.
abstract: Fourier transform surface plasmon resonance (FT-SPR) was utilized to study specific and non-specific interactions between proteins and a biotinylated polymer film by monitoring adsorptions of streptavidin (SAv) and bovine serum albumin (BSA) on the polymer films. The biotinylated polymer, poly(lactide-co-2,2-dihydroxymethyl-propylene carbonate-graft-biotin) [P(LA-co-DHC/biotin)], was prepared by ring-opening copolymerization of lactide and a OH-bearing cyclic carbonate monomer, followed by biotinylation of the OH groups. The copolymer was coated onto the FT-SPR chip and vacuum-dried, hydrated at 70°C, and treated with a blocking agent respectively to achieve different surface status. The FT-SPR results showed that the vacuum-dried film had the most BSA adsorption; hydration treatment led to migration of the biotin moieties from inner film to surface and thus resulted in less BSA adsorption; blocking layer on the polymer surface saturated the active sites for physical and chemical adsorptions on the surface and thus weakened the BSA adsorption. Adsorption of SAv displayed similar polymer-surface-status dependence, i.e., more adsorption on vacuum-dried surface, less adsorption on hydrated surface and the least adsorption on blocked surface. Compared with BSA, SAv showed more enhanced adsorptions on P(LA-co-DHC/biotin) surface because of the specific interaction of biotin moieties in the polymer with SAv molecules, especially on the blocked surface. The above semi-quantified results further indicate that the FT-SPR system is suitable for investigating interactions between polymer surface and bio-molecules.
authors.affiliation: State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, Changchun 130022, PR China.
authors.forename: Jizheng
authors.initials: J
authors.lastname: Wei
authors.forename: Lesan
authors.initials: L
authors.lastname: Yan
authors.forename: Xiuli
authors.initials: X
authors.lastname: Hu
authors.forename: Xuesi
authors.initials: X
authors.lastname: Chen
authors.forename: Yubin
authors.initials: Y
authors.lastname: Huang
authors.forename: Xiabin
authors.initials: X
authors.lastname: Jing
mesh_terms: D000327:Adsorption; D000818:Animals; D001710:Biotin / Q000737:chemistry; D019921:Biotinylation; D002417:Cattle; D005583:Fourier Analysis; D008567:Membranes, Artificial; D015394:Molecular Structure; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry; D019809:Streptavidin / Q000737:chemistry; D020349:Surface Plasmon Resonance; D013499:Surface Properties
chemical_list: D008567:Membranes, Artificial; D011108:Polymers; D012710:Serum Albumin, Bovine; D001710:Biotin; D019809:Streptavidin