title: Initial Events Associated with Virus PBCV-1 Infection of Chlorella NC64A.
abstract: Chlorella viruses (or chloroviruses) are very large, plaque-forming viruses. The viruses are multilayered structures containing a large double-stranded DNA genome, a lipid bilayered membrane, and an outer icosahedral capsid shell. The viruses replicate in certain isolates of the coccal green alga, Chlorella. Sequence analysis of the 330-kbp genome of Paramecium bursaria Chlorella virus 1 (PBCV-1), the prototype of the virus family Phycodnaviridae, reveals <365 protein-encoding genes and 11 tRNA genes. Products of about 40% of these genes resemble proteins of known function, including many that are unexpected for a virus. Among these is a virus-encoded protein, called Kcv, which forms a functional K(+) channel. This chapter focuses on the initial steps in virus infection and provides a plausible role for the function of the viral K(+) channel in lowering the turgor pressure of the host. This step appears to be a prerequisite for delivery of the viral genome into the host.
authors.affiliation: Institute of Botany, Technische Universitat Darmstadt, 64287, Darmstadt, Germany.
authors.forename: Gerhard
authors.initials: G
authors.lastname: Thiel
authors.forename: Anna
authors.initials: A
authors.lastname: Moroni
authors.forename: David
authors.initials: D
authors.lastname: Dunigan
authors.forename: James L
authors.initials: JL
authors.lastname: Van Etten