title: Detection of specific DNA from crude extracts of rice seed grains using matrix-assisted laser desorption ionization time-of-flight mass spectrometry.
abstract: To rapidly detect specific genes, crude extracts prepared from rice seed grains were used as templates for PCR, the PCR products were digested with restriction enzymes or urasil-DNA glycosylase, and then matrix-assisted laser desorption ionization mass spectrometry (MALDI-TOF MS) was used to detect amplified DNA. It was possible to amplify small DNA fragments (50-60bp), but not large ones (>200bp), using crude extracts as the PCR template. This method can be completed within 1h, including extractions, and is well suited to automation for high-throughput analyses.
authors.affiliation: National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305-8602, Japan. kajiwara@affrc.go.jp
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Kajiwara
mesh_terms: D018744:DNA, Plant / Q000032:analysis* / Q000235:genetics; D000939:Epitopes / Q000235:genetics; D017343:Genes, Plant / Q000235:genetics; D012275:Oryza / Q000737:chemistry* / Q000235:genetics; D010936:Plant Extracts / Q000737:chemistry*; D030821:Plants, Genetically Modified; D016133:Polymerase Chain Reaction; D055315:Prolamins / Q000235:genetics; D012639:Seeds / Q000737:chemistry* / Q000235:genetics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D018744:DNA, Plant; D000939:Epitopes; D010936:Plant Extracts; D055315:Prolamins