title: Novaeguinoside II inhibits cell proliferation and induces apoptosis of human brain glioblastoma U87MG cells through the mitochondrial pathway.
abstract: Novaeguinoside II is one of the newly found asterosaponins, which are a kind of steroidal glycosides extracted from starfish with an anti-tumor effect. In this study, we investigated the bioactivity of novaeguinoside II on human U87MG glioblastoma cells. The results showed that novaeguinoside II significantly suppresses U87MG cell proliferation in a time- and concentration-dependent manner. Flow cytometric analysis of DNA in U87MG cells showed that novaeguinoside II induces the prominent appearance of an S phase peak in the cell cycle that is suggestive of apoptosis that is identical to the result of an annexin V/PI assay. Fluorescence and electron microscopy revealed apoptotic change of U87MG cells. The electrophoresis of DNA showed a typical "ladder" that is consistent with apoptotic DNA fragmentation. Cytofluorometry showed a decreased mitochondrial transmembrane potential in novaeguinoside II-treated U87MG cells. Western blot showed that novaeguinoside II increased the expression of cytochrome-c and caspase-3 protein. The novel findings suggest that novaeguinoside II can induce apoptosis of U87MG cells by a mitochondrial apoptotic pathway.
authors.affiliation: Institute of Neurosurgery, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, People's Republic of China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhou
authors.forename: Gang
authors.initials: G
authors.lastname: Cheng
authors.forename: Guang
authors.initials: G
authors.lastname: Cheng
authors.forename: Hai-Feng
authors.initials: HF
authors.lastname: Tang
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhang
mesh_terms: D017304:Annexin A5 / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D045304:Cytochromes c / Q000378:metabolism; D053938:DNA Fragmentation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry / Q000379:methods; D005909:Glioblastoma / Q000473:pathology / Q000648:ultrastructure; D006801:Humans; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D046529:Microscopy, Electron, Transmission; D008928:Mitochondria / Q000187:drug effects*; D012503:Saponins / Q000494:pharmacology*; D013997:Time Factors
chemical_list: D017304:Annexin A5; D012503:Saponins; C499567:novaeguinoside II; D045304:Cytochromes c