title: Heparin for adhesion prevention: comparison of three different dosages with Seprafilm in a murine model.
abstract: BACKGROUND
  This study aimed to assess the safety and effectiveness of different dosages of heparin for adhesion prevention by comparing with Seprafilm, in a murine model.
  
  
  METHODS
  Seventy five Balb/c mice were randomized into five groups. Group C were reserved as controls, and 62.5 IU, 125 IU, 250 IU of heparin, and Seprafilm were intraperitoneally applied in studied groups. The severity and locations of adhesions were assessed after the sacrification on day 14. The cause of death was investigated to evaluate the side effects of the drugs.
  
  
  RESULTS
  The death of 2 subjects due to peritonitis (1 in Group C, 1 in Group H62.5) left 14 subjects in Group C and Group H62.5 (P ≥ 0.05), and no hemorrhage related death was observed. The use of the products significantly reduced the severity score of adhesion and the number of animals, had adhesions in different locations of the abdominal cavity, when the results were compared with the control group (P < 0.05 for all comparisons). Higher dosages of heparin seemed to be more effective. The results in group S, groups H250 and H125 were quite similar.
  
  
  CONCLUSIONS
  Relatively high doses (125 IU and 250 IU) of intra-abdominal heparin may be comparable in safety and effectiveness to Seprafilm in adhesion prevention in mice.
authors.affiliation: Department of General Surgery, Kartal Education and Research Hospital, Istanbul, Turkey. mkement@yahoo.com
authors.forename: Metin
authors.initials: M
authors.lastname: Kement
authors.forename: Zafer
authors.initials: Z
authors.lastname: Censur
authors.forename: Mustafa
authors.initials: M
authors.lastname: Oncel
authors.forename: Mehmet E
authors.initials: ME
authors.lastname: Buyukokuroglu
authors.forename: Fazli C
authors.initials: FC
authors.lastname: Gezen
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005343:Fibrinolytic Agents / Q000008:administration & dosage*; D006493:Heparin / Q000008:administration & dosage*; D006820:Hyaluronic Acid / Q000008:administration & dosage*; D008567:Membranes, Artificial; D051379:Mice; D008807:Mice, Inbred BALB C; D000267:Tissue Adhesions / Q000517:prevention & control*
chemical_list: D005343:Fibrinolytic Agents; D008567:Membranes, Artificial; C118198:Seprafilm; D006820:Hyaluronic Acid; D006493:Heparin