title: Evaluation of endogenous reference genes for analysis of gene expression with real-time RT-PCR during planarian regeneration.
abstract: It is important that endogenous reference genes for real-time RT-PCR be empirically evaluated for stability in different cell types, developmental stages, and/or sample treatment. To select the most stable endogenous reference genes during planarian regeneration, three housekeeping genes, 18S rRNA, ACTB and DjEF2, were identified and established expression levels by real-time RT-PCR. The data were analyzed by GeNorm and NormFinder software. Expression levels of the Djsix-1 gene were studied in parallel with ACTB and DjEF2 both or each and 18S rRNA as reference during regeneration. The results showed that ACTB was the most stable expressed reference gene in the planarian regeneration.
authors.affiliation: Henan Normal University, Xinxiang 453007, People's Republic of China.
authors.forename: Yan-Qing
authors.initials: YQ
authors.lastname: Yuwen
authors.forename: Zi-Mei
authors.initials: ZM
authors.lastname: Dong
authors.forename: Qing-Hua
authors.initials: QH
authors.lastname: Wang
authors.forename: Xiao-Juan
authors.initials: XJ
authors.lastname: Sun
authors.forename: Chang-Ying
authors.initials: CY
authors.lastname: Shi
authors.forename: Guang-Wen
authors.initials: GW
authors.lastname: Chen
mesh_terms: D000818:Animals; D018507:Gene Expression Regulation, Developmental; D010932:Planarians / Q000235:genetics* / Q000502:physiology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods* / Q000592:standards*; D012015:Reference Standards; D012038:Regeneration / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods* / Q000592:standards*; D012984:Software; D013997:Time Factors; D014158:Transcription, Genetic
chemical_list: D012333:RNA, Messenger