title: Ameliorative effects of mulberry (Morus alba L.) leaves on hyperlipidemia in rats fed a high-fat diet: induction of fatty acid oxidation, inhibition of lipogenesis, and suppression of oxidative stress.
abstract: To determine the effects of mulberry (Morus alba L.) leaves on hyperlipidemia, we performed gene expression profiling of the liver. Rats were fed a high-fat diet and administered mulberry leaves for 7 weeks. Plasma triglyceride and non-esterified fatty acid levels were significantly lower in the rats treated with mulberry leaves as compared with the untreated rats. DNA microarray analysis revealed that mulberry leaves upregulated expression of the genes involved in α-, β- and ω-oxidation of fatty acids, mainly related to the peroxisome proliferator-activated receptor signaling pathway, and downregulated the genes involved in lipogenesis. Furthermore, treatment with mulberry leaves upregulated expression of the genes involved in the response to oxidative stress. These results indicate that consumption of fatty acids and inhibition of lipogenesis are responsible for the reduction in plasma lipids caused by mulberry administration. In addition, mulberry treatment maintains the body's oxidative state at a low level despite enhancing fatty acid oxidation.
authors.affiliation: Chemistry Division, Kanagawa Prefectural Institute of Public Health, Chigasaki, Kanagawa, Japan.
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Kobayashi
authors.forename: Maki
authors.initials: M
authors.lastname: Miyazawa
authors.forename: Asuka
authors.initials: A
authors.lastname: Kamei
authors.forename: Keiko
authors.initials: K
authors.lastname: Abe
authors.forename: Takashi
authors.initials: T
authors.lastname: Kojima
mesh_terms: D000818:Animals; D001692:Biological Transport / Q000187:drug effects / Q000235:genetics; D003998:Dicarboxylic Acids / Q000378:metabolism; D004041:Dietary Fats / Q000009:adverse effects*; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism*; D005419:Flavonoids / Q000378:metabolism; D020869:Gene Expression Profiling; D005990:Glycerol / Q000378:metabolism; D015228:Hypertriglyceridemia / Q000209:etiology / Q000378:metabolism*; D007657:Ketone Bodies / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D050155:Lipogenesis / Q000187:drug effects* / Q000235:genetics; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D008297:Male; D031625:Morus / Q000737:chemistry*; D020411:Oligonucleotide Array Sequence Analysis; D010084:Oxidation-Reduction / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects* / Q000235:genetics; D010636:Phenols / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry*; D059808:Polyphenols; D051381:Rats; D017208:Rats, Wistar
chemical_list: D003998:Dicarboxylic Acids; D004041:Dietary Fats; D005227:Fatty Acids; D005419:Flavonoids; D007657:Ketone Bodies; D010636:Phenols; D010936:Plant Extracts; D059808:Polyphenols; D005990:Glycerol