title: Maladaptive defensive behaviours in monoamine oxidase A-deficient mice.
abstract: Rich evidence indicates that monoamine oxidase (MAO) A, the major enzyme catalysing the degradation of monoamine neurotransmitters, plays a key role in emotional regulation. Although MAOA deficiency is associated with reactive aggression in humans and mice, the involvement of this enzyme in defensive behaviour remains controversial and poorly understood. To address this issue, we tested MAOA knockout (KO) mice in a spectrum of paradigms and settings associated with variable degrees of threat. The presentation of novel inanimate objects induced a significant reduction in exploratory approaches and increase in defensive behaviours, such as tail-rattling, biting and digging. These neophobic responses were context-dependent and particularly marked in the home cage. In the elevated plus- and T-mazes, MAOA KO mice and wild-type (WT) littermates displayed equivalent locomotor activity and time in closed and open arms; however, MAOA KO mice featured significant reductions in risk assessment, as well as unconditioned avoidance and escape. No differences between genotypes were observed in the defensive withdrawal and emergence test. Conversely, MAOA KO mice exhibited a dramatic reduction of defensive and fear-related behaviours in the presence of predator-related cues, such as predator urine or an anaesthetized rat, in comparison with those observed in their WT littermates. The behavioural abnormalities in MAOA KO mice were not paralleled by overt alterations in sensory and microvibrissal functions. Collectively, these results suggest that MAOA deficiency leads to a general inability to appropriately assess contextual risk and attune defensive and emotional responses to environmental cues.
authors.affiliation: Department of Pharmacology and Pharmaceutical Sciences, School of Pharmacy, University of Southern California, Los Angeles, CA 90089, USA.
authors.forename: Sean C
authors.initials: SC
authors.lastname: Godar
authors.forename: Marco
authors.initials: M
authors.lastname: Bortolato
authors.forename: Roberto
authors.initials: R
authors.lastname: Frau
authors.forename: Mona
authors.initials: M
authors.lastname: Dousti
authors.forename: Kevin
authors.initials: K
authors.lastname: Chen
authors.forename: Jean C
authors.initials: JC
authors.lastname: Shih
mesh_terms: D000374:Aggression; D000818:Animals; D001362:Avoidance Learning; D001522:Behavior, Animal; D004924:Escape Reaction; D005106:Exploratory Behavior; D007527:Isoenzymes / Q000172:deficiency / Q000235:genetics / Q000502:physiology; D008297:Male; D018782:Maze Learning; D051379:Mice; D018345:Mice, Knockout; D008817:Mice, Mutant Strains; D008995:Monoamine Oxidase / Q000172:deficiency* / Q000235:genetics / Q000502:physiology*; D009043:Motor Activity; D050505:Mutant Proteins / Q000235:genetics / Q000502:physiology*; D017354:Point Mutation; D011897:Random Allocation; D051381:Rats; D020318:Rats, Long-Evans
chemical_list: D007527:Isoenzymes; D050505:Mutant Proteins; D008995:Monoamine Oxidase