title: Reversed-phase liquid chromatographic determination of tramiprosate in rat plasma using evaporative light scattering detector.
abstract: A simple and reliable method was developed for determining blood concentrations of tramiprosate using reversed-phase HPLC with evaporative light scattering detection. The optimum evaporator and nebulizer temperatures for ELSD were set at 90 and 60°C respectively. The method was linear for a concentration range of 10-1000 µg/mL when 0.3 mL blood was used. The intra-assay precision ranged from 1.84 to 5.24%, and the coefficient of variation (CV) for a quality control sample at 10 µg/mL was 5.24% with a bias of -9.50% from the target value. The inter-assay precision ranged from 2.58 to 5.96%, and the CV for a quality control sample at 10 µg/mL was 5.96% with a bias of -7.50% from the target value. The method described here is suitable for management of tramiprosate in Alzheimer disease therapy.
authors.affiliation: HPLC/UV Group, Analytical Chemistry Division, Discovery Laboratory, Indian Institute of Chemical Technology, Tarnaka, Hyderabad 500607, India. rnrao@iict.res.in
authors.forename: R Nageswara
authors.initials: RN
authors.lastname: Rao
authors.forename: Pawan K
authors.initials: PK
authors.lastname: Maurya
authors.forename: Dhananjay D
authors.initials: DD
authors.lastname: Shinde
authors.forename: Sachin B
authors.initials: SB
authors.lastname: Agwane
mesh_terms: D000818:Animals; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D004355:Drug Stability; D016018:Least-Squares Analysis; D008027:Light; D016014:Linear Models; D008297:Male; D009330:Nebulizers and Vaporizers; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012542:Scattering, Radiation; D013654:Taurine / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics; D013696:Temperature
chemical_list: D013654:Taurine; C001355:tramiprosate