title: Dentine sialoprotein and collagen I expression after experimental pulp capping in humans using emdogain gel.
abstract: OBJECTIVE
  To characterize the hard tissue formed in human teeth experimentally pulp capped either with calcium hydroxide or with Emdogain Gel (Biora AB, Malmö, Sweden) - a derivative of enamel matrix (EMD), using two markers for dentine; dentine sialoprotein (DSP) and type 1 collagen (Col I).
  
  
  METHODS
  Affinity-purified rabbit anti-Col I and anti-DSP polyclonal antibodies were used to stain histological sections from nine pairs of contra-lateral premolars that had been experimentally pulp amputated and randomly capped with EMDgel or calcium hydroxide. Twelve weeks after the teeth had been pulp capped, they were extracted, fixed, demineralized and serially sectioned prior to immunohistochemical staining.
  
  
  RESULTS
  In the calcium hydroxide treated teeth DSP was seen in the new hard tissue which formed a bridge. DSP was also seen in the newly formed hard tissue in the EMDgel-treated teeth. Proliferated pulp tissue partly filled the space initially occupied by EMDgel and DSP-stained hard tissue was observed alongside exposed dentine surfaces as well as in isolated masses within the proliferated pulp tissue, although the new hard tissue did not cover the pulp exposure. DSP staining was also seen in the cells lining the hard tissue in both groups. Col I staining was seen in the newly formed hard tissue in both groups.
  
  
  CONCLUSIONS
  The new hard tissue formed after pulp capping with EMDgel or calcium hydroxide contained DSP and Col I, considered to be markers for dentine. Thus, the newly formed hard tissue can be characterized as dentine rather than unspecific hard tissue.
authors.affiliation: Department of Endodontics, Faculty of Odontology, Malmö University, Malmö, Sweden. helena.fransson@mah.se
authors.forename: H
authors.initials: H
authors.lastname: Fransson
authors.forename: K
authors.initials: K
authors.lastname: Petersson
authors.forename: J R
authors.initials: JR
authors.lastname: Davies
mesh_terms: D000293:Adolescent; D001641:Bicuspid; D002126:Calcium Hydroxide / Q000494:pharmacology; D002648:Child; D024042:Collagen Type I / Q000187:drug effects* / Q000378:metabolism; D003746:Dental Enamel Proteins / Q000494:pharmacology*; D003785:Dental Pulp Capping / Q000379:methods; D003804:Dentin / Q000187:drug effects* / Q000378:metabolism; D003810:Dentinogenesis / Q000187:drug effects; D016326:Extracellular Matrix Proteins / Q000187:drug effects* / Q000378:metabolism; D005782:Gels; D006801:Humans; D016555:Matched-Pair Analysis; D010750:Phosphoproteins / Q000187:drug effects* / Q000378:metabolism; D057872:Pulp Capping and Pulpectomy Agents / Q000494:pharmacology*; D012016:Reference Values; D012038:Regeneration / Q000187:drug effects; D012795:Sialoglycoproteins / Q000187:drug effects* / Q000378:metabolism; D016896:Treatment Outcome; D000099311:Dentin Sialophosphoprotein
chemical_list: D002126:Calcium Hydroxide; D024042:Collagen Type I; D003746:Dental Enamel Proteins; D016326:Extracellular Matrix Proteins; D005782:Gels; D010750:Phosphoproteins; D057872:Pulp Capping and Pulpectomy Agents; D012795:Sialoglycoproteins; D000099311:Dentin Sialophosphoprotein; C109069:enamel matrix proteins