title: [In situ enrichment and diversity analysis of polycyclic aromatic carbon degrading bacteria in the coastal seawater of Xiamen Island].
abstract: OBJECTIVE
  The aim of this study is to assess the diversity of polycyclic aromatic carbon (PAH)-degrading bacteria in the coastal seawater of Xiamen Island.
  
  
  METHODS
  The phenanthrene-degrading bacteria were enriched by suspending phenanthrene-coated Polyvinylchloride (PVC) plates in the seawater close to Xiamen International Cruise Dock. PCR-DGGE and 16S rRNA gene clone library were used to analyze the bacteria colonizing on the PVC plates. Further, PAH-degrading bacteria were re-enriched in lab after the in situ enrichment and subjected to diversity analysis and bacterial isolation.
  
  
  RESULTS
  After 6 days incubation, the genus Cycloclasticus was shown to be the dominant bacterium on the phenanthrene (Phe)-coated plates, which accounted for 50% of the total clones in 16S rRNA gene clone library. However, on the control plates without Phe-coating, bacteria of Rhodobacteraceae were the dominant member, which accounted for 47% of the total clones. PCR-DGGE results reconfirmed the genus Cycloclasticus as the dominant member on the Phe-coated plates. After re-enrichment with Phe in laboratory, 14 strains were isolated from the consortium, which contained a potential novel species of genus Novosphingobium, named strain B-14 and identified as a Phe degrader. However, the most predominant member Cycloclasticus can not be cultivated into pure culture.
  
  
  CONCLUSIONS
  Genus Cycloclasticus is the most important PAH-degrading bacterium in the coast sea water of Xiamen Island. This is the first evidence to our knowledge about the in situ enrichment PAH-degrading bacterium in seawater.
authors.affiliation: College of Life Science and Technology, State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China. ligaoken@yahoo.com.cn
authors.forename: Liang
authors.initials: L
authors.lastname: Chen
authors.forename: Chunming
authors.initials: C
authors.lastname: Dong
authors.forename: Jin
authors.initials: J
authors.lastname: He
authors.forename: Zongze
authors.initials: Z
authors.lastname: Shao
mesh_terms: D001419:Bacteria / Q000378:metabolism*; D001673:Biodegradation, Environmental; D044822:Biodiversity; D002681:China; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D020459:Genes, rRNA / Q000235:genetics; D014644:Genetic Variation; D005843:Geography; D011084:Polycyclic Aromatic Hydrocarbons / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000032:analysis*; D041902:Rhodobacteraceae / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D012623:Seawater; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D011084:Polycyclic Aromatic Hydrocarbons; D012336:RNA, Ribosomal, 16S