title: Degradation of acetaminophen by Delftia tsuruhatensis and Pseudomonas aeruginosa in a membrane bioreactor.
abstract: The incidence and fate of pharmaceuticals in the water cycle impose a growing concern for the future reuse of treated water. Because of the recurrent global use of drugs such as Acetaminophen (APAP), an analgesic and antipyretic drug, they are often detected in wastewater treatment plant (WWTP) effluents, receiving surface waters and drinking water resources. In this study, the removal of APAP has been demonstrated in a membrane bioreactor (MBR) fed with APAP as the sole carbon source. After 16 days of operation, at a hydraulic retention time (HRT) of 5 days, more than 99.9% removal was obtained when supplying a synthetic WWTP effluent with 100 μg APAP L(-1). Batch experiments indicated no sorption of APAP to the biomass, no influence of the WWTP effluent matrix, and the capability of the microbial consortium to remove APAP at environmentally relevant concentrations (8.3 μg APAP L(-1)). Incubation with allylthiourea, an ammonia monooxygenase inhibitor, demonstrated that the APAP removal was mainly associated with heterotrophic bacteria and not with the ammonia-oxidizing bacteria. Two APAP degrading strains were isolated from the MBR biomass and identified as Delftia tsuruhatensis and Pseudomonas aeruginosa. During incubation of the isolates, hydroquinone - a potentially toxic transformation product - was temporarily formed but further degraded and/or metabolized. These results suggest that the specific enrichment of a microbial consortium in an MBR operated at a high sludge age might be a promising strategy for post-treatment of WWTP effluents containing pharmaceuticals.
authors.affiliation: Laboratory of Microbial Ecology and Technology (LabMET), Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, B-9000 Gent, Belgium.
authors.forename: Bart
authors.initials: B
authors.lastname: De Gusseme
authors.forename: Lynn
authors.initials: L
authors.lastname: Vanhaecke
authors.forename: Willy
authors.initials: W
authors.lastname: Verstraete
authors.forename: Nico
authors.initials: N
authors.lastname: Boon
mesh_terms: D000082:Acetaminophen / Q000302:isolation & purification / Q000378:metabolism*; D001673:Biodegradation, Environmental; D018533:Biomass; D019149:Bioreactors / Q000382:microbiology*; D002851:Chromatography, High Pressure Liquid; D042742:Delftia / Q000302:isolation & purification / Q000378:metabolism*; D008567:Membranes, Artificial; D058465:Nitrification; D011550:Pseudomonas aeruginosa / Q000302:isolation & purification / Q000378:metabolism*; D012015:Reference Standards; D013997:Time Factors; D014466:Ultraviolet Rays; D018508:Water Purification / Q000295:instrumentation* / Q000379:methods*
chemical_list: D008567:Membranes, Artificial; D000082:Acetaminophen