title: Solid phase extraction of lactic acid from fermentation broth by anion-exchangeable silica confined ionic liquids.
abstract: Three anion-exchangeable, silica-confined ionic liquids were synthesized for solid phase extraction of lactic acid from fermentation broth, followed by high-performance liquid chromatography coupled to ultraviolet detection. By comparing the adsorption isotherms of lactic acid on different silica-confined ionic liquids, interactions between the lactic acid and sorbents were investigated. The adsorbed amounts were then fitted into different adsorption isotherm equations; finally, the Langmuir equation was selected. Then the imidazolium silica with the highest adsorption capacity of lactic acid was packed into a cartridge for solid phase extraction. The loading volume of the cartridge was optimized by the Langmuir equation and geometry. After washing with distilled water and eluting with 0.25 mol L(-1) of an HCl solution, the lactic acid was separated from interference with a recovery yield of 91.9%. Furthermore, this kind of anion-exchangeable material exhibited potential for industrial applications and separation of other anionic bioactive compounds.
authors.affiliation: Department of Chemical Engineering, Inha University, 253 Yonghyun-Dong, Nam-Ku, Incheon 402-751, South Korea.
authors.forename: Wentao
authors.initials: W
authors.lastname: Bi
authors.forename: Jun
authors.initials: J
authors.lastname: Zhou
authors.forename: Kyung Ho
authors.initials: KH
authors.lastname: Row
mesh_terms: D000327:Adsorption; D017743:Equipment Reuse; D005285:Fermentation; D007474:Ion Exchange; D052578:Ionic Liquids / Q000737:chemistry*; D019344:Lactic Acid / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification*; D015203:Reproducibility of Results; D012822:Silicon Dioxide / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000295:instrumentation / Q000379:methods*; D013057:Spectrum Analysis
chemical_list: D052578:Ionic Liquids; D019344:Lactic Acid; D012822:Silicon Dioxide