title: Transcript profiling in M. truncatula lss and sunn-1 mutants reveals different expression profiles despite disrupted SUNN gene function in both mutants.
abstract: A novel autoregulation of nodulation locus in Medicago truncatula, lss, silences the SUNN gene thorough a cis-acting mechanism. Microarray analysis was performed on the Affymetrix Gene Chip® Medicago Genome Array with cDNA isolated from seven-day-old seedlings of wild type, sunn-1 and lss plants. The results suggest that in lss plants expression of only a few dozen genes differs significantly from wild type while in sunn-1 plants expression of several hundred genes represented by over 800 probe sets is altered. These results suggest that the kinase domain modification caused by the sunn-1 mutation alters the receptor's influence on gene expression and that these differences are present even in the absence of nodulation.
authors.affiliation: Department of Genetics & Biochemistry, Clemson University, Clemson, SC, USA.
authors.forename: Elise
authors.initials: E
authors.lastname: Schnabel
authors.forename: Lucinda
authors.initials: L
authors.lastname: Smith
authors.forename: Sharon
authors.initials: S
authors.lastname: Long
authors.forename: Julia
authors.initials: J
authors.lastname: Frugoli
mesh_terms: D020869:Gene Expression Profiling; D046913:Medicago truncatula / Q000235:genetics*; D009154:Mutation; D010940:Plant Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*
chemical_list: D010940:Plant Proteins; D012333:RNA, Messenger