title: [Changes of ion channel in rat hippocampal neurons during primary culture].
abstract: OBJECTIVE
  Whole-cell recording technique was used to observe the changes of voltage-dependent ion channels and NMDA receptor currents of rat hippocampal neurons during primary culture.
  
  
  RESULTS
  There was no significant difference of voltage-dependent Na+ current (I(Na)) at 7 d, 14 d and 21 d in culture. It's the same for delayed rectifier K+ current (Ik). However, voltage-dependent Ca2+ current (I(Ca)) and its density were continuously and markedly increased. Further studies showed that the increase of I(Ca) was resulted from the increase of L-type voltage-dependent Ca2+ channels (L-VDCC). NMDA receptor current was also significantly increased with time of culture.
  
  
  CONCLUSIONS
  Ca2+ influx through VDCC and NMIDA receptor is the fatal factor in the aging and death of hippocampal neurons.
authors.affiliation: Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Yang
authors.forename: Zhen-wei
authors.initials: ZW
authors.lastname: Liu
authors.forename: Qin
authors.initials: Q
authors.lastname: Wan
authors.forename: Wen-xia
authors.initials: WX
authors.lastname: Zhou
authors.forename: Yong-xiang
authors.initials: YX
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002118:Calcium / Q000378:metabolism; D020746:Calcium Channels, L-Type / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D016922:Cellular Senescence; D006624:Hippocampus / Q000166:cytology*; D007473:Ion Channels / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D018408:Patch-Clamp Techniques; D051381:Rats; D017208:Rats, Wistar; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism; D013997:Time Factors
chemical_list: D020746:Calcium Channels, L-Type; D007473:Ion Channels; D016194:Receptors, N-Methyl-D-Aspartate; D002118:Calcium