title: In vitro synthesis and characterization of bacteriochlorophyll-f and its absence in bacteriochlorophyll-e producing organisms.
abstract: Bacteriochlorophyll(BChl)-f which has not yet been found in natural phototrophs was prepared by chemically modifying chlorophyll-b. The retention time of reverse-phase high-performance liquid chromatography of the synthetic monomeric BChl-f as well as its visible absorption and fluorescence emission spectra in a solution were identified and compared with other naturally occurring chlorophyll pigments obtained from the main light-harvesting antenna systems of green sulfur bacteria, BChls-c/d/e. Based on the above data, BChl-f was below the level of detection in three strains of green photosynthetic bacteria producing BChl-e.
authors.affiliation: Department of Bioscience and Biotechnology, Faculty of Science and Engineering, Ritsumeikan University, Kusatsu, Shiga 525-8577, Japan. tamiaki@se.ritsumei.ac.jp
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Tamiaki
authors.forename: Jun
authors.initials: J
authors.lastname: Komada
authors.forename: Michio
authors.initials: M
authors.lastname: Kunieda
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Fukai
authors.forename: Taichi
authors.initials: T
authors.lastname: Yoshitomi
authors.forename: Jiro
authors.initials: J
authors.lastname: Harada
authors.forename: Tadashi
authors.initials: T
authors.lastname: Mizoguchi
mesh_terms: D001429:Bacteriochlorophylls / Q000096:biosynthesis* / Q000138:chemical synthesis / Q000737:chemistry; D019414:Chlorobi / Q000378:metabolism*; D002734:Chlorophyll / Q000737:chemistry / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D018724:Spinacia oleracea / Q000737:chemistry
chemical_list: D001429:Bacteriochlorophylls; C120930:bacteriochlorophyll e; C120931:bacteriochlorophyll f; D002734:Chlorophyll; C037184:chlorophyll b