title: Different innate signatures induced in human monocyte-derived dendritic cells by wild-type dengue 3 virus, attenuated but reactogenic dengue 3 vaccine virus, or attenuated nonreactogenic dengue 1-4 vaccine virus strains.
abstract: DNA microarrays were used to assess the innate gene signature in human myeloid dendritic cells infected with chimeric dengue 1-4 vaccines, a wild-type dengue 3 virus, or a classically attenuated serotype 3 vaccine shown to be reactogenic in humans. We observed a very reproducible signature for each of the 4 chimeric dengue vaccines, involving stimulation of type I interferon and associated genes, together with genes encoding chemokines and other mediators involved in the initiation of adaptive responses. In contrast, wild-typeDEN3 virus induced a predominantly inflammatory profile, while the reactogenic attenuated serotype 3 vaccine appeared to induce a blunted response.
authors.affiliation: Research and Development, Sanofi Pasteur, 69280, Marcy l'Étoile, France.
authors.forename: Claire
authors.initials: C
authors.lastname: Balas
authors.forename: Audrey
authors.initials: A
authors.lastname: Kennel
authors.forename: Florence
authors.initials: F
authors.lastname: Deauvieau
authors.forename: Regis
authors.initials: R
authors.lastname: Sodoyer
authors.forename: Nadege
authors.initials: N
authors.lastname: Arnaud-Barbe
authors.forename: Jean
authors.initials: J
authors.lastname: Lang
authors.forename: Bruno
authors.initials: B
authors.lastname: Guy
mesh_terms: D015415:Biomarkers; D016207:Cytokines / Q000096:biosynthesis; D003713:Dendritic Cells / Q000276:immunology* / Q000821:virology*; D003715:Dengue / Q000175:diagnosis* / Q000517:prevention & control*; D053059:Dengue Vaccines / Q000276:immunology*; D003716:Dengue Virus / Q000276:immunology*; D003937:Diagnosis, Differential; D020869:Gene Expression Profiling; D006801:Humans; D046228:Microarray Analysis; D009000:Monocytes / Q000276:immunology / Q000821:virology; D014613:Vaccines, Attenuated / Q000276:immunology
chemical_list: D015415:Biomarkers; D016207:Cytokines; D053059:Dengue Vaccines; D014613:Vaccines, Attenuated