title: 9-Piperazine substituted perylene-3,4-dicarboximide as a fluorescent probe in ratiometric analysis.
abstract: The communication describes the synthesis and studies of 9-piperazine substituted perylene-3,4-dicarboximide (1). In aqueous solution, the ratio of fluorescence emission intensities from the localized excited state (∼585 nm) to the charge transfer excited state (∼695 nm) increases in the pH range of 7.5-9. Furthermore, compound 1 may be used for ratiometric detection of double-stranded DNA.
authors.affiliation: Department of Chemistry, University of Nevada, Reno, Reno, NV 89557, USA.
authors.forename: Liming
authors.initials: L
authors.lastname: Huang
authors.forename: Suk-Wah
authors.initials: SW
authors.lastname: Tam-Chang
mesh_terms: D004247:DNA / Q000032:analysis*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D010569:Perylene / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry; D000077489:Piperazine; D010879:Piperazines / Q000138:chemical synthesis / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes; D010879:Piperazines; D000077489:Piperazine; D010569:Perylene; D004247:DNA