title: [Myocardial protection by non-invasive limb preconditioning: the role of nuclear factor kappa-B].
abstract: OBJECTIVE
  To investigate a possible role for the transcription factor nuclear factor kappa-B (NF-kappaB) in preconditioning of the heart to ischemia by remote, early protection.
  
  
  METHODS
  48 Wistar rats were randomly divided into three experimental groups. In group I/R, the rats underwent 30 min occlusion of the left anterior descending coronary artery, and 120 min reperfusion. In group PL, the rats underwent four cycles of 5 min occlusion and reperfusion of both hind limbs using a tourniquet before the experiment was continued as in Group I/R. In Group P(L-D), we administered NF-kappaB specific inhibitor, ProDTC 125 mg/kg peritoneally, 15 min before IPG. Infarct size as a percentage of the area at risk was determined by triphenyltetrazolium chloride staining. And another 8 rats in each group were killed and myocardium were stored in liquid nitrogen for the measurement of NF-kappaB mRNA.
  
  
  RESULTS
  The myocardial infarct size (IS) was decreased significantly in Group PL compared with group I/R, and the IS/AAR was 34.5% +/- 7.6% and 58.5% +/- 8.5%, respectively ( P < 0.05). The IS/AAR was 54.4% +/- 8.9% in group P(L-D), and there was no significant difference compared with group I/R (P > 0.05). The NF-kappaBmRNA was weaker in P(L) group than that in I/R Group,but is stronger than that in P(L-D) group (P < 0.05). There was almost no expression of NF-kappaB mRNA in P(L-D) group.
  
  
  CONCLUSIONS
  Noninvasive limb IPC is effective in protecting the myocardium from ischemia reperfusion injury. NF-kappaB plays an important role in the mechanism of this acute remote preconditioning.
authors.affiliation: Department of Anesthesiology, The First Affiliated Hospital, China Medical University, Shenyang 110001, China. chxg2000@yahoo.com
authors.forename: Xiao-Guang
authors.initials: XG
authors.lastname: Chen
authors.forename: Hong
authors.initials: H
authors.lastname: Ma
authors.forename: Jun-Ke
authors.initials: JK
authors.lastname: Wang
authors.forename: Bin-Yang
authors.initials: BY
authors.lastname: Wu
mesh_terms: D000818:Animals; D005121:Extremities / Q000098:blood supply; D019157:Ischemic Preconditioning, Myocardial / Q000379:methods*; D008297:Male; D009203:Myocardial Infarction / Q000473:pathology; D009206:Myocardium / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D016328:NF-kappa B