title: [Influence of myocardial ischemia/reperfusion in vitro on nuclear envelope NTPase activity and mRNA export in the cardiomyocyte of rabbits].
abstract: OBJECTIVE
  To study the alteration of nuclear envelope associated NTPase activity and mRNA nucleocytoplasmic transport in rabbit cardiomyocyte following in vitro ischemia and reperfusion.
  
  
  METHODS
  The model of myocardial perfusion in rabbit was used to produce myocardial I/R injury and cardiomyocyte nuclear envelope vesicles were prepared by density gradient centrifugation for the assay of mRNA transport rate and NTPase activity.
  
  
  RESULTS
  NTPase activity was reduced and mRNA transport rate was significantly decreased in I/R (P < 0.01) but not in ischemia group( P > 0.05).
  
  
  CONCLUSIONS
  The nuclear envelope vesicles had been injured following ischemia and reperfusion and resulted in NTPase activity reduced and egress of mRNA interrupted, and therefore may lead to decreasing of protein synthesis.
authors.affiliation: Institute of Cardiovascular Research, The First Hospital, Peking University, Beijing 100034, China.
authors.forename: C F
authors.initials: CF
authors.lastname: Xia
authors.forename: N K
authors.initials: NK
authors.lastname: Liu
authors.forename: J
authors.initials: J
authors.lastname: Yang
authors.forename: Y Z
authors.initials: YZ
authors.lastname: Pang
authors.forename: Y
authors.initials: Y
authors.lastname: Huo
authors.forename: C S
authors.initials: CS
authors.lastname: Tang
mesh_terms: D021581:Active Transport, Cell Nucleus; D000818:Animals; D066298:In Vitro Techniques; D017202:Myocardial Ischemia / Q000378:metabolism*; D015425:Myocardial Reperfusion; D032383:Myocytes, Cardiac / Q000378:metabolism; D009685:Nuclear Envelope / Q000378:metabolism*; D043583:Nucleoside-Triphosphatase / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D011817:Rabbits
chemical_list: D012333:RNA, Messenger; D043583:Nucleoside-Triphosphatase