title: [Establishment of a HEK293 cell line stably expressing human HCN2 gene].
abstract: OBJECTIVE
  To create a model for studying ionic channels by means of the expressing human HCN2 and G418-resistant HEK293 cell lines established.
  
  
  METHODS
  pcDNA3-hHCN2 was transfected with Lipofectin2000 into HEK293 cell line. The transfected cells would be survived in the further culture medium containing G418 antibiotic as the hHCN2 gene could express a G418 resistant products. Whole-cell patch clamp investigated that hHCN2 gene was transfected into HEK293 cells.
  
  
  RESULTS
  The G418 resistant (600 ug/ml) HEK293 cell line was established successfully and whole-cell patch clamp recorded ionic currents of transfected hHCN2.
  
  
  CONCLUSIONS
  The G418 resistant HEK293 cell line was successfully established with transfection of plasmid pcDNA3-hHCN2 by Lipofectin, which might be useful for studying the relationship between the structure and function of cloned ionic channels.
authors.affiliation: Department Cardiovascular Research, The First Affiliated Hospital to Suzhou University, Suzhou 215006, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhao
authors.forename: Xiang-Jun
authors.initials: XJ
authors.lastname: Yang
authors.forename: Xia
authors.initials: X
authors.lastname: Bai
authors.forename: Hong-Xia
authors.initials: HX
authors.lastname: Li
authors.forename: Xu-Jie
authors.initials: XJ
authors.lastname: Cheng
authors.forename: Wen-Ping
authors.initials: WP
authors.lastname: Jiang
mesh_terms: D015870:Gene Expression; D005822:Genetic Vectors; D057809:HEK293 Cells; D006801:Humans; D064428:Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels; D007473:Ion Channels / Q000235:genetics*; D018408:Patch-Clamp Techniques; D010957:Plasmids; D015221:Potassium Channels; D014162:Transfection
chemical_list: C578710:HCN2 protein, human; D064428:Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels; D007473:Ion Channels; D015221:Potassium Channels