title: [Detection of distinct proteins for brain injury rats serum using a protein chip].
abstract: OBJECTIVE
  To explore the alteration of serum protein fingerprinting of rats subjected brain injury.
  
  
  METHODS
  Male Sprague-Dawley rats were randomly divided into seven groups, ie, normal control group, sham operation group, injury 4 h, 8 h, 12 h, 24 h and 48 h groups. The change of protein pattern in serum was monitored with weak cationic exchanger chip and surface-enhanced laser desorption ionization-time of flight-mass spectrometry.
  
  
  RESULTS
  The reduction of 5648 Da protein expression was detected at 4 h, 8 h or 12 h after injury (P < 0.01, compared with control and sham operation) and recovery at 24 h or 48 h. The increase of 9681 Da protein expression was detected at 4 h, 8 h or 12 h after injury (P < 0.05, compared with control and sham operation) and recovery at 24 h or 48 h.
  
  
  CONCLUSIONS
  The alteration of protein expression in serum could be induced after brain injury.
authors.affiliation: Department of Pathology, Medical College of Chinese People's Armed Police Forces, Tianjin 300162, China.
authors.forename: Qing-Ming
authors.initials: QM
authors.lastname: Shu
authors.forename: Yong-Liang
authors.initials: YL
authors.lastname: Zhang
authors.forename: Ling-Zhi
authors.initials: LZ
authors.lastname: Li
authors.forename: Bing
authors.initials: B
authors.lastname: He
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Li
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000378:metabolism*; D001930:Brain Injuries / Q000097:blood*; D004195:Disease Models, Animal; D008297:Male; D040081:Protein Array Analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D044967:Serum / Q000378:metabolism
chemical_list: D001798:Blood Proteins