title: The interactions of astrocytes and fibroblasts with defined pore structures in static and perfusion cultures.
abstract: Open pores to maintain nutrient diffusion and waste removal after cell colonization are crucial for the successful application of constructs based on assembled membranes, in our case tubular scaffolds made of ɛ-polycaprolactone (PCL), for use in tissue engineering. Due to the complex three-dimensional structure and large size of such scaffolds needed for transplantable tissues, it is difficult to investigate the cell-pore interactions in situ. Therefore miniaturized bioreactors inside Petri dishes (30 mm in diameter), containing porous PCL or poly-dimethylsiloxane (PDMS) membranes, were developed to allow the interactions of different cells with defined pores to be investigated in situ during both static and perfusion cultures. Investigation of two different cell types (fibroblasts and cortical astrocytes) and how they interact with a range of pores (100-350 μm in diameter) for up to 50 days indicated that the cells either 'covered' or 'bridged' the pores. Three distinct behaviors were observed in the way cortical astrocytes interacted with pores, while fibroblasts were able to quickly bridge the pores based on consistent "joint efforts". Our studies demonstrate that the distinct pore sealing behaviors of both cell types were influenced by pore size, initial cell density and culture period, but not by medium perfusion within the range of shear forces investigated. These findings form important basic data about the usability of pores within scaffolds that could inform the design and fabrication of suitable scaffolds for various applications in tissue engineering.
authors.affiliation: Centre for Cell Engineering, Institute of Molecular, Cellular and Systems Biology, College of Medical, Veterinary and Life Sciences, Joseph Black Building, University of Glasgow, Glasgow G12 8QQ, UK. tao.sun@glasgow.ac.uk
authors.forename: Tao
authors.initials: T
authors.lastname: Sun
authors.forename: Peter S
authors.initials: PS
authors.lastname: Donoghue
authors.forename: Jennifer R
authors.initials: JR
authors.lastname: Higginson
authors.forename: Nikolaj
authors.initials: N
authors.lastname: Gadegaard
authors.forename: Susan C
authors.initials: SC
authors.lastname: Barnett
authors.forename: Mathis O
authors.initials: MO
authors.lastname: Riehle
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000378:metabolism*; D001672:Biocompatible Materials / Q000737:chemistry; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods*; D002540:Cerebral Cortex / Q000166:cytology; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D008422:Materials Testing; D010477:Perfusion; D011091:Polyesters / Q000737:chemistry; D016062:Porosity; D051381:Rats; D017207:Rats, Sprague-Dawley; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D004129:Dimethylpolysiloxanes; D011091:Polyesters; C016240:polycaprolactone; C013830:baysilon