title: A poloxamine-polylysine acrylate scaffold for modular tissue engineering.
abstract: A new polymer, poloxamine-polylysine acrylate (PPA), was synthesized for tissue-engineering applications. Polylysine was used to confer endothelial cell attachment properties to the poloxamine-based polymer, while the acrylate groups made it photo-cross-linkable. The PPA polymer was synthesized in three reaction steps. (1) Polylysine was acrylated using N-hydroxysuccinimide acrylic acid ester and the reaction product was characterised by (1)H-NMR. (2) The hydroxyl groups on poloxamine were activated by tresylation with tresyl chloride and the reaction product (tresylated poloxamine) was characterised by ICP-AES for sulphur content. (3) The acrylated polylysine was reacted with the activated poloxamine to obtain the final product, poloxamine-polylysine acrylate. The final product was characterised by CHN elemental analysis. Aqueous solutions containing a mixture of PPA and poloxamine methacrylate were photo-cross-linked by exposure to a 365 nm UV light source in the presence of a photoinitiator to obtain hydrogels. The synthesized PPA polymer enhanced endothelial cell adhesion on poloxamine-based, photo-cross-linked hydrogel scaffolds. The same synthesis methods can be used in the future to introduce other desired functions (through other peptides) into the scaffold biomaterial.
authors.affiliation: a Department of Chemical Engineering and Applied Chemistry, Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON, Canada.
authors.forename: Ema C
authors.initials: EC
authors.lastname: Ciucurel
authors.forename: Michael V
authors.initials: MV
authors.lastname: Sefton
mesh_terms: D000179:Acrylates / Q000737:chemistry; D002448:Cell Adhesion; D016923:Cell Death; D002460:Cell Line; D042783:Endothelial Cells / Q000502:physiology; D006801:Humans; D020100:Hydrogels / Q000138:chemical synthesis / Q000737:chemistry; D008422:Materials Testing; D015394:Molecular Structure; D055668:Photochemical Processes; D011107:Polylysine / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D066244:Proton Magnetic Resonance Spectroscopy; D012996:Solutions; D023822:Tissue Engineering / Q000295:instrumentation* / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry; D014466:Ultraviolet Rays; D014867:Water / Q000737:chemistry
chemical_list: D000179:Acrylates; D020100:Hydrogels; D011108:Polymers; D012996:Solutions; D014867:Water; D011107:Polylysine