title: Implant surface treatments affect gene expression of Runx2, osteogenic key marker.
abstract: BACKGROUND
  The aim of this study was to study the effects of various surface treatments to a titanium surface on the expression of Runx2 in vitro.
  
  
  METHODS
  Human Osteosarcoma TE-85 cells were cultured on machined, sandblasted, or anodic oxidized cpTi discs. At various times of incubation, the cells were collected and then processed for the analysis of mRNA expression of Runx2 using reverse transcription-PCR.
  
  
  RESULTS
  The expression pattern of Runx2 mRNA was differed according to the types of surface treatment. When the cells were cultured on the untreated control culture plates, the gene expression of Runx2 was not increased during the experiments. In the case of that the cells were cultured on the machined cpTI discs, the expression level was intermediate at the first day, but increased constitutively to day 5. In cells on sandblasted cpTi discs, the expression level was highest in the first day sample and the level was maintained to 5 days. In cells on anodized cpTi discs, the expression level increased rapidly to 3 days, but decreased slightly in the 5-th day sample.
  
  
  CONCLUSIONS
  Different surface treatments may contribute to the regulation of osteoblast function by influencing the level of gene expression of key osteogenic factors.
authors.affiliation: Graduate student, Department of Dental Prosthodontics, Seoul National University College of Dentistry, Seoul, Korea.
authors.forename: Young
authors.initials: Y
authors.lastname: Na
authors.forename: Seong-Joo
authors.initials: SJ
authors.lastname: Heo
authors.forename: Seong-Kyun
authors.initials: SK
authors.lastname: Kim
authors.forename: Jai-Young
authors.initials: JY
authors.lastname: Koak