title: Replication and transcription of human papillomavirus type 58 genome in Saccharomyces cerevisiae.
abstract: BACKGROUND
  To establish a convenient system for the study of human papillomavirus (HPV), we inserted a Saccharomyces cerevisiae selectable marker, Ura, into HPV58 genome and transformed it into yeast.
  
  
  RESULTS
  HPV58 genome could replicate extrachromosomally in yeast, with transcription of its early and late genes. However, with mutation of the viral E2 gene, HPV58 genome lost its mitotic stability, and the transcription levels of E6 and E7 genes were upregulated.
  
  
  CONCLUSIONS
  E2 protein could participate in viral genome maintenance, replication and transcription regulation. This yeast model could be used for the study of certain aspects of HPV life cycle.
authors.affiliation: Department of Medical Microbiology, Shandong University School of Medicine, Jinan, Shandong 250012, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Xiao
authors.initials: X
authors.lastname: Wang
authors.forename: Juan
authors.initials: J
authors.lastname: Liu
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.forename: Xiao-Li
authors.initials: XL
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Tang
authors.forename: Yun-Dong
authors.initials: YD
authors.lastname: Sun
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.forename: Xiu-Ping
authors.initials: XP
authors.lastname: Yu
authors.forename: Wei-Ming
authors.initials: WM
authors.lastname: Zhao
mesh_terms: D020869:Gene Expression Profiling; D005814:Genes, Viral; D006801:Humans; D027383:Papillomaviridae / Q000502:physiology*; D012441:Saccharomyces cerevisiae / Q000821:virology*; D014158:Transcription, Genetic; D014170:Transformation, Genetic; D014773:Virology / Q000379:methods*; D014779:Virus Replication