title: Cardiac imaging using clinical 1.5 t MRI scanners in a murine ischemia/reperfusion model.
abstract: To perform cardiac imaging in mice without having to invest in expensive dedicated equipment, we adapted a clinical 1.5 Tesla (T) magnetic resonance imaging (MRI) scanner for use in a murine ischemia/reperfusion model. Phase-sensitive inversion recovery (PSIR) sequence facilitated the determination of infarct sizes in vivo by late gadolinium enhancement. Results were compared to histological infarct areas in mice after ischemia/reperfusion procedure with a good correlation (r = 0.807, P < .001). In addition, fractional area change (FAC) was assessed with single slice cine MRI and was matched to infarct size (r = -0.837) and fractional shortening (FS) measured with echocardiography (r = 0.860); both P < .001. Here, we demonstrate the use of clinical 1.5 MRI scanners as a feasible method for basic phenotyping in mice. These widely available scanners are capable of investigating in vivo infarct dimensions as well as assessment of cardiac functional parameters in mice with reasonable throughput.
authors.affiliation: Department of Internal Medicine III (Cardiology), Innsbruck Medical University, Anichstr. 35, 6020 Innsbruck, Austria.
authors.forename: Jakob G J
authors.initials: JG
authors.lastname: Voelkl
authors.forename: Bernhard J
authors.initials: BJ
authors.lastname: Haubner
authors.forename: Christian
authors.initials: C
authors.lastname: Kremser
authors.forename: Agnes
authors.initials: A
authors.lastname: Mayr
authors.forename: Gert
authors.initials: G
authors.lastname: Klug
authors.forename: Alexander
authors.initials: A
authors.lastname: Loizides
authors.forename: Silvana
authors.initials: S
authors.lastname: Müller
authors.forename: Otmar
authors.initials: O
authors.lastname: Pachinger
authors.forename: Michael
authors.initials: M
authors.lastname: Schocke
authors.forename: Bernhard
authors.initials: B
authors.lastname: Metzler
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D057791:Cardiac Imaging Techniques / Q000295:instrumentation* / Q000379:methods; D004195:Disease Models, Animal; D004452:Echocardiography; D006352:Heart Ventricles / Q000473:pathology; D006571:Heterocyclic Compounds; D008279:Magnetic Resonance Imaging / Q000295:instrumentation* / Q000379:methods; D051379:Mice; D008810:Mice, Inbred C57BL; D009203:Myocardial Infarction / Q000473:pathology; D009942:Organometallic Compounds; D010641:Phenotype; D015427:Reperfusion Injury / Q000473:pathology*
chemical_list: D006571:Heterocyclic Compounds; D009942:Organometallic Compounds; C050823:gadolinium 1,4,7,10-tetraazacyclododecane-N,N',N'',N'''-tetraacetate