title: Thermodynamic characterization of the binding interaction between the histone demethylase LSD1/KDM1 and CoREST.
abstract: Flavin-dependent histone demethylases catalyze the posttranslational oxidative demethylation of mono- and dimethylated lysine residues, producing formaldehyde and hydrogen peroxide in addition to the corresponding demethylated protein. In vivo, histone demethylase LSD1 (KDM1; BCH110) is a component of the multiprotein complex that includes histone deacetylases (HDAC 1 and 2) and the scaffolding protein CoREST. Although little is known about the affinities of or the structural basis for the interaction between CoREST and HDACs, the structure of CoREST(286-482) bound to an α-helical coiled-coil tower domain within LSD1 has recently been reported. Given the significance of CoREST in directing demethylation to specific nucleosomal substrates, insight into the molecular basis of the interaction between CoREST and LSD1 may suggest a new means of inhibiting LSD1 activity by misdirecting the enzyme away from nucleosomal substrates. Toward this end, isothermal titration calorimetry studies were conducted to determine the affinity and thermodynamic parameters characterizing the binding interaction between LSD1 and CoREST(286-482). The proteins tightly interact in a 1:1 stoichiometry with a dissociation constant (K(d)) of 15.9 ± 2.07 nM, and their binding interaction is characterized by a favorable enthalpic contribution near room temperature with a smaller entropic penalty at pH 7.4. Additionally, one proton is transferred from the buffer to the heterodimeric complex at pH 7.4. From the temperature dependence of the enthalpy change of interaction, a constant-pressure heat capacity change (ΔC(p)) of the interaction was determined to be -0.80 ± 0.01 kcal mol(-1) K(-1). Notably, structure-driven truncation of CoREST revealed that the central binding determinant lies within the segment of residues 293-380, also known as the CoREST "linker" region, which is a central isolated helix that interacts with the LSD1 coiled-coil tower domain to create a triple-helical bundle. Thermodynamic parameters obtained from the binding between LSD1 and the linker region of CoREST are similar to those obtained from the interaction between LSD1 and CoREST(286-482). These results provide a framework for understanding the molecular basis of protein-protein interactions that govern nucleosomal demethylation.
authors.affiliation: Department of Chemistry, Levine Science Research Center, Duke University, Durham, NC 27708, USA.
authors.forename: Sunhee
authors.initials: S
authors.lastname: Hwang
authors.forename: Allison A
authors.initials: AA
authors.lastname: Schmitt
authors.forename: Andrea E
authors.initials: AE
authors.lastname: Luteran
authors.forename: Eric J
authors.initials: EJ
authors.lastname: Toone
authors.forename: Dewey G
authors.initials: DG
authors.lastname: McCafferty
mesh_terms: D002151:Calorimetry; D056970:Co-Repressor Proteins; D019277:Entropy; D056466:Histone Demethylases / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009419:Nerve Tissue Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D009707:Nucleosomes / Q000201:enzymology / Q000235:genetics; D011485:Protein Binding / Q000235:genetics; D025941:Protein Interaction Mapping / Q000379:methods; D017434:Protein Structure, Tertiary / Q000235:genetics; D011994:Recombinant Proteins / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism; D012097:Repressor Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D013379:Substrate Specificity / Q000235:genetics; D020349:Surface Plasmon Resonance; D013816:Thermodynamics
chemical_list: D056970:Co-Repressor Proteins; D009419:Nerve Tissue Proteins; D009707:Nucleosomes; C121019:RCOR1 protein, human; D011994:Recombinant Proteins; D012097:Repressor Proteins; D056466:Histone Demethylases; C495195:KDM1A protein, human