title: Uniform culture in solid-state fermentation with fungi and its efficient enzyme production.
abstract: Solid-state fermentation (SSF) has attracted a lot of interest for carrying out high-level protein production in filamentous fungi. However, it has problems such as the fermentation heat generated during the culture in addition to the reduced mobility of substances. These conditions lead to a nonuniform state in the culture substrate and result in low reproducibility. We constructed a non-airflow box (NAB) with a moisture permeable fluoropolymer membrane, thereby making it possible to control and maintain uniform and optimal conditions in the substrate. For the NAB culture in Aspergillus oryzae, temperature and water content on/in the whole substrate were more consistent than for a traditional tray box (TB) culture. Total weight after the culture remained constant and dry conditions could be achieved during the culture. These data demonstrate the possibility of growing a uniform culture of the whole substrate for SSF. The NAB is advantageous because it allows for the control of exact temperature and water content in the substrate during the culture by allowing vapor with latent heat to dissipate out of the box. In addition, several enzymes in the NAB culture exhibited higher production levels than in the TB culture. We believe that culturing in the constructed NAB could become a standard technique for commercial SSF.
authors.affiliation: Industrial Technology Center of Okayama Prefecture, 5301 Haga, Kita-ku, Okayama 701-1296, Japan. kazunari_itou@pref.okayama.lg.jp
authors.forename: Kazunari
authors.initials: K
authors.lastname: Ito
authors.forename: Tomoka
authors.initials: T
authors.lastname: Kawase
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Sammoto
authors.forename: Katsuya
authors.initials: K
authors.lastname: Gomi
authors.forename: Masahiro
authors.initials: M
authors.lastname: Kariyama
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Miyake
mesh_terms: D001236:Aspergillus oryzae / Q000201:enzymology*; D019149:Bioreactors / Q000382:microbiology*; D004798:Enzymes / Q000096:biosynthesis*; D005285:Fermentation; D005656:Fungal Proteins / Q000096:biosynthesis; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007218:Industrial Microbiology / Q000295:instrumentation / Q000379:methods*; D013696:Temperature; D014867:Water / Q000378:metabolism
chemical_list: D004798:Enzymes; D005656:Fungal Proteins; D014867:Water