title: [Effect of Salvia Miltiorrhiza Bunge on expression of osteoprotegerin in cultured human periodontal ligament fibroblasts].
abstract: OBJECTIVE
  To study the effect of Salvia Miltiorrhiza Bunge on the expression of osteoprotegerin (OPG) in cultured human periodontal ligament fibroblasts.
  
  
  METHODS
  Primary culture of human periodontal ligament fibroblasts was established and the fifth passage cells were used in this study. Concentration-dependent effect of Salvia Miltiorrhiza Bunge on OPG mRNA and OPG protein secretion were determined by reverse transcription-polymerase chain reaction (RT-PCR) and enzyme linked immunosorbant assay (ELISA), respectively. The data were analyzed with SPSS15.0 software package.
  
  
  RESULTS
  OPG mRNA expression was increased after 1 h exposure to various concentrations of Salvia Miltiorrhiza Bunge compared with the control group (P<0.05). OPG protein secretion was significantly increased after 12 h exposure to various concentrations of Salvia Miltiorrhiza Bunge compared with the control group (P<0.05).
  
  
  CONCLUSIONS
  Salvia Miltiorrhiza Bunge can up-regulate the expression of OPG in cultured human periodontal ligament fibroblasts.
authors.affiliation: The Second Hospital affiliated to Zhejiang Chinese Medical College, Hangzhou 310005, Zhejiang Province, China. dhj333@sina.com
authors.forename: Hong-jiang
authors.initials: HJ
authors.lastname: Du
authors.forename: Xue-peng
authors.initials: XP
authors.lastname: Chen
authors.forename: Hong-hai
authors.initials: HH
authors.lastname: Yan
mesh_terms: D005347:Fibroblasts; D006801:Humans; D053244:Osteoprotegerin; D010513:Periodontal Ligament; D053245:RANK Ligand; D012333:RNA, Messenger; D027561:Salvia miltiorrhiza
chemical_list: D053244:Osteoprotegerin; D053245:RANK Ligand; D012333:RNA, Messenger